LOCUS       DASSLZ010000001         4405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_126705, whole genome shotgun sequence.
ACCESSION   DASSLZ010000001 DASSLZ010000000
VERSION     DASSLZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126705"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1410)
                     /locus_tag="VFM00_00005"
     CDS             complement(190..1410)
                     /locus_tag="VFM00_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-independent formaldehyde
                     dehydrogenase"
                     /protein_id="HET7496659.1"
                     /translation="MKALVYRGPRDIRVEDVPDPQIQESTDVLVRITSTNICGSDLHM
                     YEGRTNVEKGKVLGHENLGEVIEVGEAVRLVQVGDRVCVPFNVSCGCCSNCERGYTGA
                     CLTMNPPNAGAAYGYAGMGPYDGGQAEYLRVPYGDFNCLVLPEDAADKESDYVMLADI
                     FPTGYEAAERACVVPGDTVVVYGAGPVGLMAAYSSYLRGAARVMVVDRIPARLALVEK
                     IGAIPIDDSKASPVDRVLELTDGEGADKGCECVGYQAHDVEGHEHPNMTMNNLVESVR
                     PTGSIGVVGVFTPEDPKSPDRLEKQGQIAFEMGRFFEKGLTMGSGQANVKRWNRKLMQ
                     LIHHGKARPSLIVSHELPLAAGPDAYKQFDARVSGWTKVILKPQVATAKRREMTAEEP
                     LPKKQEERESVYAS"
     gene            complement(1519..1941)
                     /locus_tag="VFM00_00010"
     CDS             complement(1519..1941)
                     /locus_tag="VFM00_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496660.1"
                     /translation="MKKLILPLLVLSLFGLNAVTAHAASPKSTTYTFTVMDTEGGKSN
                     ASGTAVFQPLPNGKTIVHVRVEGLQPGSQYFLTWSTSTGCTLETDNTAKTFNHFTAKT
                     NGSLNYTTRLDTDLSAIGSLGIRTETGQVLVACATATP"
     gene            2411..3091
                     /locus_tag="VFM00_00015"
     CDS             2411..3091
                     /locus_tag="VFM00_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496661.1"
                     /translation="MNAIGPSSPDERELMRSLHEGSMAALGALYRRTAPYLYPLAVRI
                     VGSRERACAVIEDLFEEIWRDRARWKSAVEIPAVDWISRCRELALAQTGPDPRGSDPR
                     GIDPRGSDPRHPDASHAEPRRPMTEPMASSSVLTPAPTAEGALSDPRLVAREALASLP
                     ETDRRALEEAYFHGAHAREVAVLIGAATSDAAMILRSALVRFREHIAEQGHVAGREAA
                     PAATGSAS"
     gene            3088..3933
                     /locus_tag="VFM00_00020"
     CDS             3088..3933
                     /locus_tag="VFM00_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7496662.1"
                     /translation="MIEATALRALGILGGEPLVRLMGRLREGDEALEDDLREMERTAS
                     LLALAAPPAEPPAELERRVLSRVAAEGYYFLHERESEWEPSKVPGAWLRRLFIHARES
                     RETRLVRLTASAPASAVADLAGCGFYLLSGDLHVDGERLEIGDYYHAIGEAPTGRTDM
                     GCVLFSVLAGAPSRGKSRSRSVVRASEGAWKEIAPGVTTKRLGEDSERALRTYLLRIE
                     PGASWESHRHEGTEELFLVRGDWRCLGTNLHPGDYHRAGPGTEHETTTSAAGCKLIVV
                     SHSLE"
     gene            3938..>4405
                     /locus_tag="VFM00_00025"
     CDS             3938..>4405
                     /locus_tag="VFM00_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496663.1"
                     /translation="MAERATGTAAERPQAPRPANDRPQATRSSNGDATPADRIAETLR
                     LKGPLTAAEIAQEHGCGAVAVRSQLRNLEAAGYVARNTERRPVGRPVSRYALTGNAES
                     LFPKRYDLFSAHLVDAIVEEFGEEGLVRILERWEEGLHERLDAELPRDPRTRLE"
BASE COUNT          680 a         1568 c         1480 g          677 t
ORIGIN      
        1 agcgggcccc acaagcccac aaccaagccc ccacggtgcc cgcatcaggg ggccacataa
       61 cgaagcggcc ccggcgatag acaccggagc cgccgcgtcc catgtgaatg gaggggggac
      121 tgggggaatc gagctccaag agcctctttc cgagacgggc cgcggcagcg ccacggccgg
      181 ccgcgagcct cagctggcgt acacgctctc gcgttcctcc tgcttcttcg gcaacggctc
      241 ctcggccgtc atctcgcgcc gcttcgccgt cgccacctgc ggcttcagga tcaccttcgt
      301 ccagccactc acgcgcgcgt cgaactgctt gtaggcgtcc ggccccgcgg ccagcggcag
      361 ctcgtgggag acgatcagcg acggacgggc tttcccgtgg tggatcagct gcatgagctt
      421 ccggttccac cgcttcacgt tggcctgccc cgagcccatg gtgagcccct tctcgaagaa
      481 gcggcccatc tcgaaggcga tctgcccctg cttctccagg cggtcgggac tcttcggatc
      541 ctcgggggtg aagacgccca ccaccccgat cgaccccgtg ggacggaccg actccacgag
      601 attgttcatc gtcatgttgg gatgctcgtg cccctcgacg tcgtgcgcct ggtagccgac
      661 gcactcgcag cccttgtcgg cgccctcgcc gtcggtcagc tcgagcacgc gatccacggg
      721 cgaggccttg gagtcgtcga tcgggatcgc cccgatcttc tcgaccaacg ccagccgcgc
      781 ggggatccgg tccacgacca tcacgcgcgc cgccccgcgc aggtaggacg agtaggcagc
      841 catcagaccc accgggcccg cgccgtacac gaccaccgtg tcgccgggca cgacgcaggc
      901 gcgctcggcg gcctcgtagc cggtcgggaa gatgtccgcc agcatcacgt agtccgattc
      961 cttgtcggcg gcgtcctcgg gcaggacgag gcagttgaag tcgccgtagg gcacgcgcag
     1021 gtattcggcc tgccccccgt cgtaggggcc catgccggcg tagccgtagg cggcccctgc
     1081 gttgggggga ttcatcgtca agcaggctcc ggtgtagccg cgctcgcagt tgctgcaaca
     1141 gccgcagctc acgttgaacg gaacgcacac ccggtctccg acctggacca ggcgcacggc
     1201 ctcgcccacc tcgatcactt ccccgaggtt ttcgtggcca agcaccttcc ccttctcgac
     1261 gttggtgcgt ccttcgtaca tgtgcagatc ggagccgcag atgttcgtgc tggtgatgcg
     1321 caccagcacg tcggtggact cctggatttg cggatccggc acgtcctcga cgcggatgtc
     1381 gcgcggaccg cgatagacga gtgctttcat aggacctccg cccgacgggc ggagcccgga
     1441 gacttcccgg gctccgctcc cgccagcgag ttcactccgg acgaggcgtc aacgaacgac
     1501 gcgccgagga acgacgcgtc agggcgtcgc cgtggcgcat gccaccagaa cctgtcccgt
     1561 ctcggtccgg atcccgagcg agccgatcgc gctcaggtcg gtgtccagac gggtcgtgta
     1621 gttcagcgag ccgttcgtct tcgccgtgaa atgattgaac gtcttggccg tgttgtccgt
     1681 ctcgagggtg catccggtgc tcgtcgacca ggtcaggaaa tactggctgc ccggctgaag
     1741 gccctccacg cgcacgtgga cgatcgtctt gccgttggga agcggctgga ataccgcggt
     1801 ccccgaggcg ttcgacttgc caccctcggt gtccatcacc gtgaaggtgt aggtcgtgct
     1861 cttgggggac gccgcgtgcg cggtgaccgc attcaaaccg aacagggaaa ggacaagcag
     1921 aggaaggatc agcttcttca tgacggtctc ctttggccga tatcttgcga acagggaaaa
     1981 ctgtgccact tcagggcctt tgcactgatg gtccccgagg gacggcggcg gcaccccttc
     2041 gcgccgctgt gccgttccac acagggagtg ttccatccgg agcaggcacc ttgaagccgg
     2101 cgaccacgat ctcggttcca gccctcgcgc ttcgcttccc gcgaagggca tcgggctgtg
     2161 gagagagggt ggctgcaggt agcgggccag agtcccaggc tggaatcggc cgcatgtcgg
     2221 ggccttgcag acgcccctgg aaatcgaagc cgaggctgga tccagcctca cttttcttga
     2281 tcgcgccggg acgcgggagc agacttgatc cgcgggccga acgtggaagc agggctgatc
     2341 cgcgtaccga accgtggaag cggggcttga tccgcggacc gaaccgcagg gaagcacccg
     2401 gggagacgac atgaacgcca tcggaccttc atcccccgac gaacgcgagc tgatgcgctc
     2461 gctccacgag ggcagcatgg ccgcgctcgg agccctctat cgccgcacgg cgccctacct
     2521 ctatccgctg gcggtccgga tcgtgggctc gcgcgagcgc gcgtgcgccg tgatcgagga
     2581 tctcttcgag gagatctggc gcgaccgggc ccgctggaag agcgccgtcg agatcccggc
     2641 cgtcgactgg atctcgcgct gccgcgagct ggccctggcg cagaccgggc ccgatccgcg
     2701 cggttccgat ccgcgaggta tcgacccgcg aggctccgac ccgcgccacc ccgatgcgag
     2761 ccatgccgag ccgcggcgcc ccatgacgga gccgatggct tcctcgtcgg tcctcacccc
     2821 cgcaccgacg gcggagggcg cgctctccga tccgcgcctc gtcgcgcgcg aggcgctcgc
     2881 ttcccttccc gagaccgacc ggcgcgcgct ggaggaggcc tacttccacg gcgcgcacgc
     2941 gcgggaagtc gccgtgctga tcggcgcggc caccagcgac gccgccatga tcctccggag
     3001 cgccctcgtg cgcttccgcg agcacatcgc cgaacagggg catgtcgccg gtcgcgaagc
     3061 cgcgccggcc gcgacgggga gcgcatcatg atcgaggcca ccgcgctgcg cgcgctcggg
     3121 atcctggggg gcgagccgct cgtccgcctc atgggacgcc ttcgcgaggg ggacgaagcc
     3181 ttggaggacg acctccgcga gatggagcgg accgcctccc tgctcgcgct ggcagccccg
     3241 cccgccgagc cgcccgccga gctcgagcgc cgcgtgctct cgcgcgtggc ggccgagggc
     3301 tactacttcc tgcatgagcg ggagtcggaa tgggagccgt ccaaagttcc gggcgcgtgg
     3361 ctccgccggc tcttcatcca cgcgcgggaa tcacgcgaga cgcggctggt ccgtctgacc
     3421 gcctcagccc ccgcctccgc tgtcgcggac ctggccggct gcggcttcta tctcctctcc
     3481 ggcgacctcc acgtcgacgg ggagcgtctc gagatcgggg actactacca cgcgatcggc
     3541 gaggcgccga ccgggcgcac cgacatgggc tgcgttctct tctccgtcct ggccggcgca
     3601 ccgtcgcgcg gcaagtcccg ttcgcgatcg gtcgtgcgcg cgtccgaggg agcgtggaag
     3661 gagatcgccc ccggcgtcac gaccaaacgt ctgggagagg attcggagcg ggcgctccgg
     3721 acctatctcc ttcgcatcga gcccggcgcg agctgggagt cgcatcggca tgaggggacg
     3781 gaggagctgt ttctcgtgcg cggcgattgg cgctgcctgg gcacgaacct ccaccccggc
     3841 gactaccacc gcgccgggcc gggcaccgag catgagacca cgacctccgc ggccggctgc
     3901 aagctgatcg tcgtgagcca ctcgctggaa taacgggatg gccgagcgcg cgacaggcac
     3961 cgcggccgaa cggccccaag cgccgcgccc cgcgaacgat cggccccaag cgacgcggtc
     4021 ctcgaacgga gacgcgaccc ccgccgaccg aatcgcggaa acgctgcggc tcaagggccc
     4081 gctcaccgcc gcggagatcg cccaagagca cggctgcggt gcggtggcgg tgcgctccca
     4141 gcttcgaaac ctcgaggccg cgggctacgt ggctcgaaac accgagcgcc gtcctgtcgg
     4201 acgccccgtc tcgcgctacg ccttgaccgg aaacgccgag tcgctcttcc ccaagcgcta
     4261 cgacctgttc tcagcccacc tggtggacgc gatcgtggag gagttcggcg aagagggact
     4321 ggtccgaatc ctcgagcgct gggaggaggg ccttcacgag cgtctggacg cggagctgcc
     4381 gcgcgatccc aggacgcgac tggag
//
LOCUS       DASSLZ010000002         6300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1774347, whole genome shotgun sequence.
ACCESSION   DASSLZ010000002 DASSLZ010000000
VERSION     DASSLZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774347"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VFM00_00030"
     CDS             complement(<1..907)
                     /locus_tag="VFM00_00030"
                     /inference="COORDINATES: protein motif:HMM:NF036554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HET7496664.1"
                     /translation="MPRLFAALCAALLLAPLPAGAATAPADSAAARAPADSAAARSST
                     RTPSVKVEVLGLSGTLKKNVLASLSIADKKQRKKANEEVIRHLHARADQEIRRALQPF
                     GYYRPLIHPELITQGTWIARYTVEPGPAILLDSVLVDVTGEGENDPRYQKIVRGYPLH
                     KGDVLIQPTYDLAKSTFETAAAEGGYLDARFLTSRIDVDLERYAAAIVIRYDTGPRHY
                     FGEVSFDKDVIQEQMLHDFVTFHPGDVFDFRKLLQLQTDLSSTGYFTKVEVEPGAEPT
                     GHRVVPIGVSLAPAKKLRFTGGVGYG"
     gene            1132..1611
                     /locus_tag="VFM00_00035"
     CDS             1132..1611
                     /locus_tag="VFM00_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7496665.1"
                     /translation="MLIGVISDTHGLLRPEAREALRGVEAIVHAGDVGNPAILQALRI
                     LAPVTAVHGNVDRGVLARDLPATAILEAGGASIYVLHDLAQLDLNPRAAGFGVVVSGH
                     THAPVIREKDGVLYLNPGSAGPRRFHLPVSVATLTVEDGAPRAAIIELSIQPEGPRR"
     gene            complement(1608..3377)
                     /locus_tag="VFM00_00040"
     CDS             complement(1608..3377)
                     /locus_tag="VFM00_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7496666.1"
                     /translation="MPLTSARRAHVPLFVAVVLALASSLASGTSAAAPPSRSLAVRAT
                     PHTLRYAPQEIVVIARKGELGAASDDRPVTARAALARALAEQGLDRARRADRAPMAGA
                     DRAEVWVLSSRAPGFDPVTASAALRRTGEVLAASPNYRFGLFFTQPSDVYLTYQWYVD
                     DPNGANIHLPYAWDTARGDTSVTIAIIDTGVDTGHPDLASKIWHNRDEIPGNGIDDDG
                     NGYVDDWQGWDFGVGDDDPNPEYTSDPSGLDVGFHGTFCAGIAAAATNNGDGIAGAGW
                     NCRIMPLKVAHPDSGITSEAIAGAFAYAVDQHASVISMSFGGPGDPGVPEFFQALVDM
                     AVFGGAVCVAAAGNDGSSIKTYPAACDRVLAVAATDGANLRADFSNWGSWVDVAAPGA
                     SMWSSICRNYTFTDLDQLFYEFLFNWDSFNPYMYGDGTSFACPLTAGVCGLVRARFPS
                     LTPELVLRQIVATGDAIAYDHPIGPKVNAQRAVTQTPTAVAVNEAAPGLPRVVAVPNP
                     LQGAGEIRFALPAAARARLALYDASGRRVRTLVDGPLSAGSRAVAWDGTDDRGARVAA
                     GVYFAQLTAEGAAVRSKVVLVRP"
     gene            3408..>6300
                     /locus_tag="VFM00_00045"
     CDS             3408..>6300
                     /locus_tag="VFM00_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET7496667.1"
                     /translation="MIRDPKQIPPEAGARALVVSSDTSARLAAARQWIGSLPRDAEVL
                     VVASHGHAADEAARVDAAEHGARFGLRRATIDRLGFQVAAPALARRGAAPATSLTLAA
                     VVARAVHRLLERGAAGRFAQIAGRPGFPHAAVRTMEELRGAGMNAGDLRKALPEIADL
                     ASILEGMEREMGDLRLADRAEVLLLATAAIESGEAFPTPLPLLLLDLAPAQMVEWGLV
                     EALAARSPRVMATAPRGDAATIARLETILGVPATCPDTVEAGTSLALLKRHLFEDSGP
                     EPRDMDETVSVASWPGEARECVEIARRIQEEASAGVPFDRMAIFLRSPNSYRSHLEEA
                     LRRAAIPAHFARGALRPDPAGRALLALLACAAEGLSARRFAEYLSLGQVPDPDGVPDE
                     TWAPPEHDLAPAVAVAAAPGAPAGVGEGRAPDPDAGVSEGTLRAPWRWERLLVDAAVI
                     GGRDRWVRRLDGLIEEIALRRKDLDGDDTRAAGLERIAADLDHLRAFALPLITRLSEL
                     PASATWAVWLESLRELTAAALRRPHGVLGVLAELAPLGPVGPVDLVVVQHVRVPRLRE
                     LAAPPASRPEGAVFVAPIEMARGLLFDVVFVPGMAEKLFPPRILEDPLLPDEARRALG
                     ADLLATQDSRVQEHRLALRLAVGAAGRHAALSWPRVDVEMARARVPSFYGLEAIRAME
                     GRLPGFDELRGRGETGGSSKLGWPAPHNPARAIDDTEYDLAILGRLKDGADGAHAGAA
                     NYLLAANPHLARALRARGRRWLKKWSFADGLVDPDADALAALQRHRMGARSFSPTALE
                     RFASCPYQFFLQAVHRIQPREEAEALETIDPLTRGALFHEIQFELLTALRDRGKLPLD
                     PEHLEEAYRILEEAASRVADQWKEKVAPAIPRVWSDGIGAIRADLREWLRRSTESAHG
                     WVPHRFELSFGLADRDRPTADRSSVPDPVTILDGVRLRGSVDLVERRADG"
BASE COUNT          845 a         2205 c         2321 g          929 t
ORIGIN      
        1 tgccgtagcc gacaccgccc gtgaaccgga gcttcttcgc gggcgccagg ctcacgccga
       61 tcgggaccac tcggtggccc gtgggctcgg cgcccggttc cacctcgacc ttcgtgaaat
      121 acccggtgct gctgagatcg gtctggagct ggaggagctt ccggaaatcg aagacgtcgc
      181 cggggtggaa ggtgacgaag tcgtggagca tctgctcctg gatgacgtcc ttgtcgaagg
      241 agacctcccc gaagtagtgc cggggaccgg tgtcgtaccg gatcacgatc gcggccgcgt
      301 agcgctccag gtccacgtcg atccggctcg tgaggaagcg ggcatccagg tagccccctt
      361 cggcggccgc ggtctcgaac gtggacttgg ccaggtcgta cgtcggctgg atcaggacgt
      421 cgcccttgtg gagcggatag ccgcggacga tcttctggta gcggggatcg ttctcccctt
      481 cgcccgtcac gtccacgagg accgagtcga gcaggatcgc ggggcccggc tcgacggtgt
      541 agcgcgcgat ccaggtgccc tgcgtgatca gctcggggtg gatgaggggc cggtagtagc
      601 cgaacggctg gagggcgcgg cggatctcct ggtcggcgcg cgcgtgaaga tgccggatca
      661 cctcttcgtt ggccttcttg cgctgcttct tgtcggcgat gctcagcgag gcgaggacgt
      721 tcttcttgag ggtgccgctc agcccgagca cctccacctt gaccgatggc gtcctggtgc
      781 tcgaccgggc ggcggcggag tccgccgggg cccgggcagc ggcggaatcc gcaggggccg
      841 tggcggcgcc ggccggcagc ggggccagga gcagcgcggc gcagagggct gcgaagagtc
      901 ggggcatcgt tccttccgag atcggcgagt gccggcgtgc ggagtccggt gaaaagctac
      961 ctctattagc cagcaccgtg ccggactgga gaatggcttc cggcgtcccg gcttacggca
     1021 cttacccgcg ccggcgggtc gctatgggcc cagggggagt cccgggcgga acgaaaccgt
     1081 gtatgtttta cagagatcga acggggcgga aaggaggaag cgggtggtgg catgctgatc
     1141 ggcgtgatct ccgacacgca cgggctgctc cggccggagg cgcgcgaggc gctgcggggc
     1201 gtcgaggcga tcgtgcacgc cggcgacgtc ggcaatcccg ccattctcca agcgctccgc
     1261 atccttgcgc ccgtgacggc ggttcatggg aacgtcgacc gcggcgtgct tgcgcgcgac
     1321 ctccccgcca ccgccatcct cgaggcgggc ggcgcgtcga tctacgtgct ccacgatctg
     1381 gcccagctgg atctgaatcc gagagcggcg ggattcggcg tggtggtgag tgggcacacg
     1441 catgcgccgg tgattcggga gaaggatggc gtgctctacc tgaatccggg cagcgcgggg
     1501 ccgaggcgct tccaccttcc cgtgtcggtg gctacgctga ccgtggagga cggcgcgccg
     1561 agggccgcga tcatcgagct ttcgatccag cccgaaggcc cgcggcgcta gggccgcacc
     1621 agcaccactt tcgaacgcac cgcggcgcct tccgcggtga gctgggcgaa gtagaccccg
     1681 gccgcgaccc gagcgccgcg atcatccgtt ccatcccacg cgaccgcgcg ggaccccgcc
     1741 gacagcgggc cgtccaccag cgtgcgcacg cgcctccccg atgcgtcgta cagagccagc
     1801 cgcgctcgtg ccgccgccgg gagcgcgaaa cggatctcgc cggccccctg cagcgggttg
     1861 ggcacggcca cgacgcgagg aagcccggga gcggcctcgt tcaccgccac cgcggtcggg
     1921 gtctgcgtga ccgcgcgctg cgcgttcacc ttggggccga tcggatggtc gtaggcgatc
     1981 gcgtcgccgg tggcgacgat ctgccgcagg acgagctccg gggtgaggct cgggaagcgg
     2041 gcgcggacca gcccgcacac gcccgccgtg agcggacagg cgaaggaggt gccgtcgccg
     2101 tacatgtagg ggttgaagct gtcccagttg aacaggaact cgtagaagag ctgatcgagg
     2161 tcggtgaacg tgtagttgcg gcagatcgag gaccacatgg acgcgcccgg cgcggccacg
     2221 tcgacccagg acccccagtt cgagaagtcg gcgcgcaggt tcgcgccgtc cgtggcggcc
     2281 acggcgagca cccggtcgca cgcggcggga taggtcttga tgctgctgcc gtcgttcccc
     2341 gcggcggcca cgcaaaccgc tcccccgaac accgccatgt ccacgagcgc ctggaagaac
     2401 tcgggaacgc cggggtcgcc tggaccccca aagctcatcg agatcaccga cgcgtgctgg
     2461 tcgacggcgt aggcgaacgc gcccgcgatc gcctcggacg tgatgccgct gtcggggtga
     2521 gccaccttga gcggcatgat gcggcagttc caccccgcgc cggcgatccc gtcgccgttg
     2581 ttggtcgcgg cggcggcgat cccggcgcag aaggtgccgt ggaagcccac gtcgaggccc
     2641 gaaggatcgg aggtgtactc cggattcgga tcgtcgtcgc ccaccccgaa gtcccacccc
     2701 tgccagtcgt ccacgtagcc gttgccgtcg tcgtcgatgc cgttgccggg aatctcgtcc
     2761 cggttgtgcc agatcttcga ggccaggtcg ggatgcccgg tgtcgacgcc ggtgtcgatg
     2821 atcgcaatgg tgacggacgt gtcgccgcgc gcggtgtccc aggcgtacgg cagatgaatg
     2881 ttcgcgccgt tgggatcatc cacgtaccac tggtaggtca ggtagacgtc gctcggctgc
     2941 gtgaagaaga gaccgaaccg atagttgggg ctggccgcga ggacttctcc cgtgcggcgg
     3001 agcgccgccg aagcggttac cggatcgaac ccgggcgccc ggctggagag cacccatact
     3061 tcggcgcggt cggcgccggc catcggtgcc cggtcggcgc ggcgggcacg gtccagtccc
     3121 tgctcggcga gagcgcgggc gagcgcggca cgcgcggtca cgggccgatc atcggaggcg
     3181 gcacccagct cgcccttccg ggcgatcacg acgatctcct ggggcgcgta gcgaagcgtg
     3241 tgcggggtgg cgcggaccgc gagcgagcgc gacggcggcg cggcggcgct cgtcccgctc
     3301 gctagagacg acgcgagcgc gaggacgacg gcgacgaaca gcggaacgtg ggcccggcga
     3361 gcagacgtga gtggcatcgg gttcatcctc gtgcgaatgt tatctccgtg atccgtgatc
     3421 ccaagcagat tcccccggag gccggggcga gggcgctggt cgtctcctcg gacacctccg
     3481 cgcgcctcgc ggccgcccgt caatggatcg gctccctgcc tcgcgatgct gaggtgctcg
     3541 tggtcgcgtc ccacgggcat gcggccgacg aggccgcgcg ggtcgacgcc gcggagcatg
     3601 gggcccgctt cgggcttcgc cgcgcgacga tcgaccggct tggcttccag gtcgcggctc
     3661 ccgcccttgc ccgccgcggc gccgctcccg ccacgtcgct gaccctggcc gcggtcgtgg
     3721 cgcgcgcggt gcaccggctc ctggagcggg gcgcggcggg ccggttcgcg cagatcgccg
     3781 gccgccccgg gttcccgcac gccgcggtgc gcacgatgga ggagctgcgc ggcgccggga
     3841 tgaacgccgg cgatctccgg aaggcgctgc cggagatcgc cgacctggct tcgatcctcg
     3901 agggaatgga gcgggaaatg ggcgaccttc ggctggccga ccgcgccgag gtgctccttc
     3961 tcgccaccgc cgcgatcgaa tcgggcgagg ccttccccac gccgctgccc ctgctcctgc
     4021 tggatctcgc tcccgcgcag atggtggaat ggggcctcgt cgaggctctc gccgcgcggt
     4081 cgccgcgcgt catggccacg gctccgcgcg gagacgccgc cacgatcgcc cggctcgaga
     4141 cgatcctcgg cgttccggcc acctgccccg acacggtgga ggccgggacc tcgcttgcgc
     4201 tgttgaagcg gcatctcttc gaagactccg gtcccgagcc gcgcgacatg gacgagaccg
     4261 tgtcggtcgc ctcctggccg ggcgaggcgc gcgaatgcgt cgagatcgcg cgccggatcc
     4321 aggaagaagc atcggccgga gtcccgttcg accgcatggc gattttcctg cgttccccga
     4381 attcctatcg atcccacctg gaagaggcgc tccggcgcgc cgcgatcccc gcccacttcg
     4441 ctcgcggcgc gctgcggccc gatccagccg gtcgtgccct tctggcactc ctggcctgcg
     4501 cggcggaggg gctctcggcg cgccggttcg cggaatacct ctccctgggc caggtgcccg
     4561 atcccgacgg cgtccccgac gagacgtggg cgcctcccga gcatgacctg gcgccggcgg
     4621 tggccgtggc ggcagcgccg ggcgcgccgg cgggcgtcgg cgagggccgc gcaccggatc
     4681 cggatgccgg agtctccgaa gggacgctcc gcgcaccgtg gcggtgggag cggctgctcg
     4741 tggacgcggc ggtgatcggc ggacgcgacc gctgggtccg gcgcctcgac ggcctgatcg
     4801 aggagatcgc gctccggcga aaggacctgg acggcgacga cacgcgcgcg gccgggctcg
     4861 agcgcatcgc cgccgacctc gatcacctcc gcgccttcgc gctccccctg atcacgcggc
     4921 tctcggagct tcccgccagc gccacgtggg cggtctggct cgagagcctc cgggagctga
     4981 cggccgccgc gctccgccgc ccgcacggcg ttctcggcgt cctggccgag ctcgcgccgc
     5041 tggggccggt tggcccggtg gatctcgtcg tcgtgcagca cgtgcgggtg ccgcgccttc
     5101 gcgagctggc ggcgcccccc gcgagccggc ccgagggagc ggtgttcgtg gccccgatcg
     5161 agatggcgcg cgggctcctg ttcgacgtgg tcttcgtgcc ggggatggcc gagaagctct
     5221 tcccgccgcg catcctggag gatcccctgc tgccggacga ggcgcgccgc gccctcggtg
     5281 cggacctcct cgcgacccag gactcgcgcg tccaggagca ccggctcgcg ctccgcctcg
     5341 cggtgggcgc cgcgggacgc cacgccgcgc tctcctggcc gcgcgtggac gtggagatgg
     5401 cgcgcgcgcg cgtgccgtcg ttctacggcc tggaagcgat ccgcgcgatg gaggggcgtc
     5461 tcccgggatt cgacgagctg cgcgggcgcg gggagaccgg cggatcgtcc aaactgggat
     5521 ggccggctcc tcacaacccc gctcgcgcga tcgacgacac cgagtacgac ctggccatcc
     5581 tcggcaggct gaaggacggc gcggacggcg cgcacgcggg ggcggcgaac tacctgctcg
     5641 ccgccaatcc ccacctggcc cgcgcgctcc gcgcccgcgg gcggcgctgg ctcaagaagt
     5701 ggagcttcgc cgacgggttg gtggatcccg atgccgatgc gctggccgcg ctccagcgcc
     5761 accgcatggg cgcgcgctcc ttctcgccga ccgcgctgga gcgtttcgcc tcctgcccct
     5821 accagttctt cctccaggct gtgcatcgca tccagccgcg cgaggaggcc gaagcgctcg
     5881 agacgatcga tccgctcacg cggggcgcgc tcttccacga gatccagttc gagctgctca
     5941 ccgcgctgcg ggatcgcggg aagctgccgc tcgatcccga gcatctggag gaggcgtacc
     6001 gcattctcga ggaggccgcg agccgcgtgg ccgaccagtg gaaggagaag gtggcgcccg
     6061 cgattccacg cgtctggtcg gacggcatcg gcgcgatccg cgccgacttg cgcgagtggc
     6121 tgcggcgctc caccgagagc gcgcacggat gggtgccgca tcggttcgag ctctccttcg
     6181 gtctcgccga ccgggaccgc cccaccgccg accgttcgag cgtgcccgat cccgtgacca
     6241 tcctggatgg ggtgcgcctg cgcggatcgg tggacctggt ggagcgccgc gccgacggcg
//
LOCUS       DASSLZ010000003         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1267934, whole genome shotgun sequence.
ACCESSION   DASSLZ010000003 DASSLZ010000000
VERSION     DASSLZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267934"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFM00_00050"
     CDS             <1..888
                     /locus_tag="VFM00_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7496668.1"
                     /translation="EEIDVADVEEGAVADEAIVMSAESAEMPAEAAEPPTANAAAGGE
                     PASPEARRERLRTLQEKLRGLGPVNLLAVQDYEERRQRLNFLTGQRKDLDDARQQLLE
                     AIEKINQTASELFLKTFEEVSGHFRTVFTTLFEGGEAALMLTGDDPLEAEIEIMARPR
                     GKKPQSISLLSGGEKALTAIALLFAIYLVKPSPFCILDEVDAPLDDANVDRFVRLLRD
                     FSQKTQFIVVTHNKKTMEVADILYGVTMQEPGVSKIVSVRWNRDAEDIEEAPAAAENG
                     SGDLSEEPSELEHAETPSS"
     gene            898..2055
                     /gene="ftsY"
                     /locus_tag="VFM00_00055"
     CDS             898..2055
                     /gene="ftsY"
                     /locus_tag="VFM00_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET7496669.1"
                     /translation="MGFLDRFSQGLKKTREAVMGQAASVLRPGHKLTEEDFEKLEEAL
                     LRADVGPAATDRVLEGVRRRLAKPGAENDPAAAVREEIENLFAGAAATGAKAGVTGAA
                     AGAGAATGTAAGATTGATAGGTGGAKSTPGPDAPTETAEEPKKRSWWPGFGKPQAPPE
                     PAKPEPPAVAAPTPPAAPSPRAPHVILIVGVNGTGKTTTIAKLAHHYKKEGERVLLVA
                     CDTFRAAASDQLQIWADRVGVEIHRQKEGADPAAVAFDGMARAKNIGATRVLVDTAGR
                     LHVKANLMAELEKVRRVIDRAIPGAPHETLLVLDATTGQNGLAQVREFGKALPMTGLV
                     LTKLDGTAKGGVALAIAESLKLPLQWVGLGEGMEDLIPFDAKEFAEALIAQ"
BASE COUNT          406 a          747 c          720 g          285 t
ORIGIN      
        1 gaggagatcg acgttgccga cgtcgaggag ggcgcggttg ccgacgaagc catcgtcatg
       61 tcggccgagt ccgccgagat gcccgccgag gcggcggagc cgccgacggc gaatgcggca
      121 gcgggcggag agccggcgtc ccctgaggcg cgccgcgaac gcctgcgcac gcttcaagag
      181 aagctccgcg gcctgggtcc cgtgaacctg ctcgccgtcc aggactacga agagcgccgc
      241 cagcgtctca acttcctcac cggccagcgc aaggatctgg acgacgcgcg ccagcagctg
      301 ctggaggcga tcgagaagat caaccagacc gcgtccgagc tcttcctcaa gaccttcgag
      361 gaagtgagcg gacatttccg cacggtcttc accacgctgt tcgagggcgg agaagcggcg
      421 ctcatgctga ccggcgacga tccgctcgag gccgagatcg agatcatggc ccgcccgcgc
      481 gggaagaagc cgcagtcgat ctcgctcctg tcgggaggcg agaaggcgct caccgccatc
      541 gcgctcctgt tcgccatcta tctcgtgaag ccgtcccctt tctgcatcct ggacgaggtg
      601 gacgcgccgc tcgacgacgc caacgtggac cgcttcgtgc gcctgctccg cgacttcagc
      661 cagaagaccc agttcatcgt cgtcacccac aacaagaaga cgatggaggt cgcggacatc
      721 ctgtacggtg tcaccatgca ggagcccggc gtgtccaaga tcgtctcggt gcggtggaac
      781 cgcgacgctg aagacatcga ggaagccccc gcggccgccg agaacggatc cggggacctg
      841 agcgaggaac cctccgaact ggagcacgcc gagacccctt cgtcgtagaa tccccgggtg
      901 ggattcctcg atcggttcag ccaggggctc aagaagaccc gcgaagccgt catgggacag
      961 gcggcctccg tcctgcgccc gggacacaaa ctcaccgaag aagatttcga gaagctcgag
     1021 gaggcgctcc tccgcgccga cgtcggcccc gccgcgaccg accgcgtgct cgaaggcgtg
     1081 cgccggcggc tggccaaacc cggggcggag aacgacccgg cggcggccgt acgggaggag
     1141 atcgagaacc tcttcgcggg cgcggccgcg accggcgcca aggcgggagt gacgggcgcc
     1201 gcggctgggg ccggtgccgc gacggggacc gcggccggtg ctacgaccgg tgccacggcc
     1261 ggcggaacgg gcggagcgaa atcaacgccc ggacccgacg cgccgactga gacggccgaa
     1321 gagccgaaga agcgctcctg gtggcccggg ttcggaaaac cgcaggctcc gccggagccg
     1381 gccaagcccg agccgcctgc cgtcgccgcg cccacacctc cagccgctcc atcgccgcgc
     1441 gcgccgcacg tcatcctgat cgtcggcgtg aacggcaccg gcaagaccac gaccatcgcg
     1501 aagctagcgc accactataa gaaggaaggg gagcgcgtcc tcttggtcgc gtgcgacacc
     1561 ttccgcgccg ccgcctccga tcagctccag atctgggccg accgggtcgg cgtagagatt
     1621 caccgccaga aggagggcgc cgaccccgcc gccgtcgcct tcgacggcat ggcgcgcgcg
     1681 aagaacatcg gcgccacgcg cgtcctcgtg gacaccgccg gccgcctcca cgtgaaagcc
     1741 aacctcatgg ccgagctgga aaaagtgcgc cgcgtcatcg accgcgccat ccccggcgcc
     1801 ccgcacgaga cgcttctggt gctcgacgcc acgacagggc agaacggcct ggcccaagtg
     1861 cgcgaattcg gaaaggcgct gcccatgacc ggcctcgtcc tcacgaagct cgacggcacg
     1921 gccaagggcg gcgtcgccct cgcgatcgcc gagtcgttga agctcccgct gcagtgggtc
     1981 gggttaggcg aaggaatgga agacctgatc cccttcgacg ccaaggaatt cgccgaagcc
     2041 ctgatcgcgc agtaggagct gggccctgat tcccctgact ctcaagtaga catgatcaca
     2101 tgccctgggg catgttaatc tatatattaa atgtttttgg aggatggaga agcccgga
//
LOCUS       DASSLZ010000004         5906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_2472, whole genome shotgun sequence.
ACCESSION   DASSLZ010000004 DASSLZ010000000
VERSION     DASSLZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2472"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /gene="aspA"
                     /locus_tag="VFM00_00060"
     CDS             complement(<1..266)
                     /gene="aspA"
                     /locus_tag="VFM00_00060"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421654.1"
                     /note="catalyzes the formation of fumarate from aspartate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HET7496670.1"
                     /translation="MVETMSAAASRGGTRTERDSLGSLEVPANAYYGVQTARAVANFP
                     ISGERLHPEMVRAVTRIKIAAARANAELGVLDAKKADAIVRAGD"
     gene            complement(294..1055)
                     /gene="lipB"
                     /locus_tag="VFM00_00065"
     CDS             complement(294..1055)
                     /gene="lipB"
                     /locus_tag="VFM00_00065"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET7496671.1"
                     /translation="MILPGGSSARGVVEHPAGKPYEPPDYGPRSPIPAYDLGRVPYVA
                     ALQFQRRAVEARAAGRAPDCVYFVEHDPVITVGRAGKDSNLRLSAAEYQLRGVEVVAV
                     ERGGDVTYHGPGQLVGYPVVALDNLPCGRDLHRYLRDLEEGVIRAVTSYGLPAERRPP
                     YTGVWVGPRKIAAIGVAVRRWIAFHGFALNVNPNLAHFDWIHPCGIRHLGVGSLASLL
                     GTAPPREEVAARLAASFTEIWGRQVQRSAEDLVHV"
     gene            complement(1066..2568)
                     /locus_tag="VFM00_00070"
     CDS             complement(1066..2568)
                     /locus_tag="VFM00_00070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET7496672.1"
                     /translation="MPVTIVVPQLGESVVEGTIGKWLKQVGEPIAKDEPIVEIITDKI
                     NIELPAPTAGTLGEITAAEGTVAQVGEKLGSILAPGESLPAGGAAAPAPAHAAAGAAS
                     GGGAGAGVDAKAARAAAATPASPESRTPQGDAPLGAVAAGPRAAASAPAFGNAPSGRP
                     ASGPATNNVAPHIAPNEDGQRLSPAVRKLVKENDVDVSQIRGSGMGGRVTREDVLTYL
                     EARKVAASAPGRAPAMAPGRMDRFEPAPAAPPSQPTKAIPALAFAAPGTREEAVEPLT
                     NVRKMIAQNMVKARHTAAHCAVADEVDMTALVDMRGRLKERVQKEYGVKLTYMPFIVK
                     AVVRALKEHPILNASMTDTEVHYKKYYNIGIAVHRDQGLIVPIVRDADRKNLLQIAAD
                     IEDLGARARAEKLTLDDIQGGTFTITNAGMFGATSSVPVINVPEVGILGVHLIEERPV
                     VRDHRIVVRWMMTLVLSFDHRLVDGTPAVQFLHRVKELLEDPESWLLDAV"
     gene            complement(2603..3631)
                     /locus_tag="VFM00_00075"
     CDS             complement(2603..3631)
                     /locus_tag="VFM00_00075"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET7496673.1"
                     /translation="MKTFDTQAPAAAPADVKTITMIEAITQALHEEMERDPRIFLIGE
                     DIANYGGVFKATKGLYEKFGPMRVIDSPISENFLVQGAVGAALAGLIPSPEIQFDDFI
                     SLAVDGIVEHAAKMRYRSGGMWTCPMVIRCCCGAAVGGGLFHSQFNASWFMHSPGLVV
                     VAPATPYDAKGLLKSAFRGQNPVLYYEHKRLYRTSKGPVPTHDYTVPLGKANVVRKGA
                     HVTVISYALMLHRSLEAAEKVAAEGIDVEVIDLRTLAPYDKETILESVQRTSRAICVY
                     ESSRTMGVGAEIAAMIAEEGFSSLDAPVRRVSPPDAPQEPFAPVLADHYLPDANRIAQ
                     AIRETVAY"
     gene            complement(3628..4113)
                     /locus_tag="VFM00_00080"
     CDS             complement(3628..4113)
                     /locus_tag="VFM00_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET7496674.1"
                     /translation="MVELVLPNDANTLGNVLGGRVLHWIDLAAAIVAHRHCRQETVTV
                     SLDQMSFLAPIRVGQLAVIAARMTYTGRTSMEIRVDVACEDLLTGERRPTSTAYLTFV
                     AIDKQGRPAPVPPLILETEEERREARAAEGRREARLRARPDHPHEPEHAAKHPGETKH
                     R"
     gene            complement(4151..5116)
                     /locus_tag="VFM00_00085"
     CDS             complement(4151..5116)
                     /locus_tag="VFM00_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET7496675.1"
                     /translation="MAVSNDELKGLYYYMNLVRQVDERCRRLFKQGRFHGTYFSAVGQ
                     EATVVGPCYGLRPDDFIGIQHREIGAVITKGMPIKYLMAQLFARKDSPDRGKSHPCHY
                     GWKPMGIVTPASTIAAQTVIVTGSALAWKIQKKDNVAVAFVGEGATSNGVWHEALNFA
                     GIHKLPIVFVVQDNLWAESVPKSLGVPVDNLSERAKAYGFPGVTIDGNDLMATYETSQ
                     DAVRKARRGEGPTLIEMKTYRWYGHSEIDPANYRTQEELESWKRRDPVPRYERLLMER
                     GVVDEAFKQQTLQRIEREIEEAIEFADKSPHPDPSEILEDVYAPL"
     gene            complement(5176..>5906)
                     /locus_tag="VFM00_00090"
     CDS             complement(5176..>5906)
                     /locus_tag="VFM00_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET7496676.1"
                     /translation="SLSFAARGAEPDPALLARAEQAAESGRTVAWVGESGLEGVRLLG
                     LFEVTDPPREDAAAAVARLKARGLTPVLATGDREAPARALARAVGLDEVRAGLTPEGK
                     VALVRELQARGETVAMVGDGLNDAPALAAADLGIALGTGTDAAMEAADVTLVRGSMDG
                     ISAALDLSRATRSTVRQNLFWAFAYNVVAIPVAAGALYPAAGILLHPTVASAAMALSS
                     VSVVTNSLRLAGWRGRPAYANFER"
BASE COUNT          801 a         2077 c         2093 g          935 t
ORIGIN      
        1 tcatcgcccg cgcgcacgat ggcgtcggcc ttcttcgcgt ccagcacgcc cagctcggcg
       61 ttggcgcgcg cggccgcgat cttgatgcgt gtcaccgcgc gcaccatctc ggggtggagc
      121 cgctcgccgc tgatcgggaa gttggcgacc gcccgggcgg tctggacgcc gtaataggcg
      181 ttcgcgggga cttcgaggga accgagggaa tcgcgttcgg tgcgggtgcc gccgcgggac
      241 gccgcggcgc tcatggtctc gaccatttca attccgagcc tctcggccgg agtctacaca
      301 tgcaccaggt cctcggccga tcgctggacc tggcgccccc atatctccgt gaaggaagcg
      361 gcgaggcgcg ccgccacctc ctcgcgcggc ggcgccgtcc cgaggagcga ggccaacgac
      421 cccacgccca ggtggcggat gccgcacgga tggatccaat cgaagtgggc caggttggga
      481 ttcacgttga gcgcgaatcc gtggaaggcg atccagcggc gcacggccac gccgatcgcg
      541 gcgatcttgc gcggcccgac ccagaccccg gtatagggcg gccgccgttc ggccggcagg
      601 ccgtacgacg tcacggcgcg gatcacccct tcctcgaggt cgcgcaggta acgatggaga
      661 tcgcggccgc agggaaggtt gtcgagcgcc acgaccggat agccgacgag ctggccgggg
      721 ccgtgatagg tgacgtcgcc gccgcgctcc accgcgacca cctcgacgcc gcggagctga
      781 tactcggcgg cggagaggcg caggttcgag tccttgccgg cgcgtcccac cgtgatcacg
      841 ggatcgtgct cgacgaagta gacgcagtcg ggggcgcggc cggcggcgcg cgcttccacg
      901 gcgcggcgct ggaactggag ggcggccacg tagggcacgc ggcccaggtc gtaggcgggg
      961 atcggcgagc gcgggccgta gtcgggcggc tcgtacggct tgcccgcggg gtgttccacc
     1021 accccgcgcg cgctggagcc ccccgggagg atcatggcgt cgagtttaca ccgcgtcgag
     1081 gagccagctc tccggatcct cgagcagctc cttcacccga tggaggaact gcaccgcggg
     1141 ggtgccgtcc accagccggt ggtcgaagga aagaaccagc gtcatcatcc agcgcaccac
     1201 gatgcggtgg tcgcggacga cggggcgctc ctcgatcagg tgcacgccca ggatgcccac
     1261 ttccggcacg ttgatgaccg ggaccgacga ggtggcgccg aacatgccgg cgttggtgat
     1321 ggtgaacgtg ccgccctgga tgtcgtccag cgtcagcttc tccgcgcggg cgcgggcgcc
     1381 cagatcctcg atgtcggcgg cgatctggag caggttcttc cggtcggcgt cgcgcacgat
     1441 cggaacgatc agcccctggt cgcggtgcac ggcgatgccg atgttgtagt acttcttgta
     1501 atgcacctcg gtgtcggtca tcgacgcgtt caggatcggg tgctccttca gcgcccggac
     1561 caccgccttc acgatgaacg gcatgtaggt cagcttgacg ccgtattcct tctgcacgcg
     1621 ctccttcagc cgtcccctca tgtcgacgag ggcggtcatg tccacttcgt ccgcgacggc
     1681 gcagtgcgct gccgtgtggc gcgccttcac catgttctgc gcgatcatct tccgcacgtt
     1741 ggtgaggggc tcgaccgctt cctcgcgcgt gccgggcgcg gcgaacgcga gtgcggggat
     1801 cgccttggtc ggctgggatg gcggcgcggc cggcgcgggc tcgaagcggt ccatgcggcc
     1861 cggggccatc gcgggtgcgc gtccaggggc cgaggcggcg accttccgcg cctcgaggta
     1921 ggtgagcacg tcctcgcgcg tcacgcggcc gcccatgccg gacccgcgga tctgcgagac
     1981 gtcgacgtcg ttctccttga ccagcttccg caccgcgggg gagaggcgct ggccgtcttc
     2041 attcggcgcg atatgcggcg ccacgttgtt ggtggcgggg ccgctcgcgg gacgtcccga
     2101 gggcgcgttg ccgaaggccg gggccgaggc ggccgcgcgc ggcccggcgg ccaccgcgcc
     2161 gagcggggcg tcgccctgcg gcgtcctgct ctccggggaa gccggggtcg cggcggcggc
     2221 gcgcgcggcc ttggcgtcca cgccggcgcc cgctcctccg cccgaggccg cgcccgcggc
     2281 ggcgtgcgcc ggggccggcg cggcggcgcc tcccgcggga agcgactcgc ccggggccag
     2341 gatcgagccc agcttctcgc cgacctgcgc gacggtgccc tcggcggcgg tgatctcgcc
     2401 gagcgtgccc gcggtcgggg cggggagctc gatgttgatc ttgtcggtga tgatctcgac
     2461 gatcggctcg tccttggcga tcggctcgcc cacctgcttc agccacttgc cgatcgttcc
     2521 ctcgacgacg ctctcgccga gctgcggcac tacgatggtg acgggcattc cgttcctccg
     2581 agcgcggccc gaagccgccg gatcaatacg cgacggtttc gcggatcgcc tgggcgatcc
     2641 gattggcgtc gggcaggtag tggtcggcca gcacgggcgc gaagggctct tgcggcgcgt
     2701 ccggcggcga cacgcggcgg accggcgcgt cgagggagct gaagccctct tcggcgatca
     2761 tggccgcgat ctcggcgccc acgcccatcg tgcgcgacga ttcgtagacg cagatggcgc
     2821 gcgaggtcct ctgcaccgac tcgagaatcg tttccttgtc gtacggcgcg agcgtgcgga
     2881 ggtcgatcac ctccacgtcg atcccctcgg cggccacctt ctccgccgcc tccagggagc
     2941 ggtgcagcat gagcgcgtag gagatcaccg tgacgtgcgc tcccttgcgg accacgttcg
     3001 cttttcccag cggcaccgtg tagtcgtgcg tcggcaccgg gcccttggag gtgcggtaga
     3061 gccgcttgtg ctcgtagtag agcaccgggt tctgtccgcg gaaggccgac ttcaagagcc
     3121 ccttcgcgtc gtacggcgtc gccggcgcca ccaccacgag ccccggcgaa tgcatgaacc
     3181 acgacgcgtt gaactgcgag tggaagaggc cgccgcccac cgccgcgccg cagcagcagc
     3241 ggatgaccat ggggcaggtc cacatgccgc ccgagcggta gcgcatcttg gccgcgtgct
     3301 ccacgatgcc gtccaccgcg agcgagatga agtcgtcgaa ctggatctcg ggggagggga
     3361 tcaggcccgc gagcgcggcg cccaccgccc cctgcaccag gaagttctcg gagatgggac
     3421 tgtcgatgac gcgcatgggg ccgaactttt catacagccc cttggtcgcc ttgaacacgc
     3481 ccccgtagtt ggcgatgtcc tcgccgatca ggaagatccg cgggtcgcgc tccatctctt
     3541 cgtggagcgc ctgcgtgatc gcctcgatca tcgtgatcgt cttcacatcc gccggggcgg
     3601 ccgccggcgc ttgggtgtcg aacgtcttca tcgatgcttg gtctcccccg gatgctttgc
     3661 tgcgtgctcc ggttcgtgcg ggtggtccgg ccgtgctcga agcctcgcct cgcggcggcc
     3721 ctcggccgcg cgcgcttcgc ggcgctcttc ttccgtctcc aagatcagcg gcggcaccgg
     3781 cgccggccga ccctgcttgt cgatcgcgac gaacgtgaga tacgccgtcg aggtcgggcg
     3841 ccgctccccg gtcaggaggt cctcacacgc cacgtcgacg cggatctcca tcgaggtgcg
     3901 gcccgtatac gtcatgcgcg ccgcgatcac cgcgagctgg ccgacgcgga tcggcgcgag
     3961 aaacgacatc tggtccagcg acaccgtcac cgtctcctgc cggcagtgcc ggtgggcgac
     4021 gatggccgcc gcgagatcga tccagtggag cacgcggccg ccgaggacgt tgcccagcgt
     4081 gttcgcgtcg ttcggcagca ccagctcgac catctccacc cgcgactcgc ggggggcgcg
     4141 tccagccggg ctagagcggg gcgtagacgt cctccaggat ctcgctcgga tcggggtgcg
     4201 ggctcttgtc ggcgaactcg atcgcctcct cgatctcccg ctcgatccgc tggagcgtct
     4261 gctgcttgaa cgcctcgtcc acgacgccgc gctccatgag gagccgctcg tagcgcggca
     4321 ccgggtcgcg gcgcttccag gattccagct cttcctgcgt ccggtagttg gccggatcga
     4381 tctccgagtg gccgtaccac cggtaggtct tcatctcgat cagggtcggg ccctcgccgc
     4441 gccgcgcctt ccggaccgcg tcctgcgagg tctcgtacgt cgccatgagg tcgttgccgt
     4501 cgatggtgac gcccgggaag ccgtaggcct tggcgcgctc ggacaggttg tccaccggaa
     4561 cgcccaggct cttcggcacc gattcggccc acaggttgtc ctgcacgacg aagacgatcg
     4621 ggagcttgtg gatccccgcg aagttcagcg cctcgtgcca gaccccgttc gacgtggctc
     4681 cctcgccgac gaaggcgacg gcgacgttgt ccttcttctg gatcttccag gcgagcgcgg
     4741 agccggtgac gatcacggtc tgcgcggcga tcgtcgaggc cggcgtgacg atccccatcg
     4801 gcttccagcc gtagtggcac gggtgcgact tcccgcggtc ggggctgtcc ttccgcgcga
     4861 agagctgcgc catgaggtac ttgatcggca tgcccttggt gatgacagcg ccgatctcac
     4921 gatgctggat gccgatgaag tcgtcggggc ggagcccgta gcaggggccc acgacggtcg
     4981 cttcctggcc cacggcggag aagtaggtgc cgtggaagcg cccctgcttg aagaggcggc
     5041 ggcagcgctc gtccacctgg cgcaccaggt tcatgtagta atagaggccc ttcagctcat
     5101 cgttggaaac ggccatgctc cctccggtcc tgtctcgcgc ggaacgaggg cgaacgacct
     5161 gttccggcgc gtggattagc gctcgaaatt agcatacgcg ggccgtccgc gccacccggc
     5221 gagacggagg ctgttggtca ccaccgagac gctggagagc gccatcgcgg ccgacgcgac
     5281 cgtggggtgg agcaggatcc ccgccgcggg atagagggcg cccgccgcca cgggaatcgc
     5341 gaccacgttg taggcgaaag cccagaagag gttctgccgg accgtgctcc gggtggcccg
     5401 cgagaggtcg agggccgccg agatcccgtc catgctcccc cgcaccaggg tcacgtccgc
     5461 cgcctccatc gcggcatcgg tgccggtgcc gagcgcgatg cccaggtcgg ccgcggccag
     5521 ggccggcgcg tcgttcaagc cgtctccgac catggctacg gtctcgccgc gggcctgaag
     5581 ctcccgcacc agggccacct ttccctccgg ggtgagcccc gcccgcacct cgtccagccc
     5641 caccgcccga gccagcgcgc gcgccggcgc ttcgcggtcg ccggtggcga gcaccggcgt
     5701 gagaccgcgc gccttgagcc gggccaccgc ggccgcggca tcctcgcgcg gcgggtcggt
     5761 cacctcgaac aggccgagaa ggcgcacccc ctcgagtccg ctctccccga cccaggccac
     5821 ggtgcggccc gactcggccg cctgctcggc gcgcgcgagg agtgccgggt cgggctcggc
     5881 gccgcgcgcc gcgaaggaga gcgagc
//
LOCUS       DASSLZ010000005         3867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_889229, whole genome shotgun sequence.
ACCESSION   DASSLZ010000005 DASSLZ010000000
VERSION     DASSLZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889229"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..828
                     /locus_tag="VFM00_00095"
     CDS             220..828
                     /locus_tag="VFM00_00095"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HET7496677.1"
                     /translation="MAIPRRTDIARGPAPRVRGLLFGLALLAALGAPPASARVVAGVE
                     MPDTFRAGEQVLRLNGTAIYSKFGIRILAAGLWLDQPEEDASKILSADLPRRYVTHFF
                     RRVSAKRVRTEWFKSLEQNTPDAGEEVKEQFRTLAGWIHDFVPGDEITLVYIPGHGSD
                     VEIGGVFRGTIPGKDFAEAYFACALGPKPRLGKPFRKHLLGL"
     gene            complement(995..1984)
                     /locus_tag="VFM00_00100"
     CDS             complement(995..1984)
                     /locus_tag="VFM00_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496678.1"
                     /translation="MRRSMLALLAVAGLALSSAPCLASGASRNSLDLQGTWNWDASYR
                     AQGAGQTPRSSGYGLQLTIRSNGSYTLIERDSASAYARCAGRLRTDPNRPVAGAAASP
                     GGAAFASLTITGWEPRYDAYTAALQGRDTLLLYPARPGTGHADAAVQRFVRAGRDAEK
                     TPRLYSRALRPPRLVSEGYGAYDVAPPDSADRLARLTSPFRKAPDRDYSESMRAAYRY
                     RHDQTPAAVIGDFDGDGVYDVALYGTAGSDAEAICVLDWNRAGQVMVLGKEPAREGAD
                     PGLYLELVPKGSRVTRVGGEGVLTYDAIRAVHAGGQRELFEWNGTTLASCAEQ"
     gene            2087..3178
                     /locus_tag="VFM00_00105"
     CDS             2087..3178
                     /locus_tag="VFM00_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496679.1"
                     /translation="MKIRSLVLLALPTVLLLAGCGGDSKNPTKPAGPTRSYFMGFSSF
                     PPRASIASIVATIDAFAPHADAALIVTEPPWDSLLAGKAPDSLIRNNQVGLTNYFRSK
                     GLTRIVVSVDPTNGLDRSADSAPLVAAGRSLTEPAIQALFRSYVVAMDTLIHPQYLSV
                     ASETNLIRAAAPAPLYQAVKQVANDAYTDLRAHDANVKIFTTVQVEVAWGRLGGSGPY
                     VGINADRADFPFAQVIGLSSYPYLAGFTDPDSLPSDYYSRITSGTGLPEMVIEGGWSS
                     DSTTSPNWTVLKQRRFIAREATLLDQASVIGWFQITFTDLDLASLGFPASAAPFASLG
                     LVDVDLNPKPALSAWDAARSRNLKPPGVL"
     gene            complement(3183..>3867)
                     /locus_tag="VFM00_00110"
     CDS             complement(3183..>3867)
                     /locus_tag="VFM00_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496680.1"
                     /translation="YLQPEGWALDLWAQRSNQQASFLVAIRGQDTLYLYPGWPGGSLT
                     DALTDRYIRKAATSDGSLHPRPRSERPPRLVSSGYGYAFEFPDSFAKMYRMTFGLHQR
                     QDYPEAVRSTYPYRHDQAPSAVMGFFDDDRTLDVVRYGVNDGAVEEIVCVFDWNRAQS
                     VATLDTFPQADRPRLYLQRLLRGSSVSLPGGREHLLPRDAVLATYEDGHKAVYEWSGG
                     SFARMELAK"
BASE COUNT          674 a         1289 c         1259 g          645 t
ORIGIN      
        1 tccccacgct tggcgcgatg gaggagctcc cccgtgatga ggaattcggg cgcgggcgag
       61 tcgggcgacg gagactcggg gctgggaagg gcgggtcgat ccatgaatgg gccgggctcg
      121 actcaattat ccggcaaggt acgtagtcgg gcaagcggcc ggagaagatc gctttcccgc
      181 ttgttccggg acgcgcggac tgagagcctt tacgagacta tggccatccc acgtcgtacc
      241 gacatcgccc gaggccctgc tccgcgggtc cgagggcttc tttttgggct ggccctcctc
      301 gccgccctcg gggcgccgcc ggcaagcgcg cgtgtcgtcg ccggcgtgga gatgcccgac
      361 accttccggg cgggagaaca ggttctccgc ctgaacggca ccgccatcta ctcgaaattc
      421 gggattcgca tcctggcggc cggtctatgg ctggatcaac ccgaagagga tgcttcgaag
      481 atcctgagcg ccgacctgcc acggagatat gtgacccact tcttccggcg cgtcagcgcg
      541 aagcgcgtgc gcacggagtg gttcaagagc ctcgaacaga acactcccga cgcgggcgag
      601 gaggtgaagg agcagttccg cacgctcgcc gggtggattc acgacttcgt ccccggagac
      661 gagatcacgc tcgtctatat ccccggccat ggctcggacg tcgagatcgg tggcgtgttc
      721 cggggaacga tccccgggaa ggacttcgcc gaagcctatt tcgcctgcgc gctcggcccc
      781 aagccgcggc tcggcaagcc cttccgaaaa cacctcctcg gcctctgatc cacgcgcgct
      841 cagccggcag gacaccaaaa aagaaggccc ggccaccggc gcgctccgca ctgtcccgat
      901 gttccagcgg agcgactgcg atcggcggga cgggcccggc cttctggtct gttacttcac
      961 caaacgtgcg agcgcaggaa aacgaacacg cgaatcactg ctcggcgcac gaggcgagcg
     1021 tggtgccgtt ccactcgaaa agctcgcgct ggccgccggc gtgcacggcg cggatcgcgt
     1081 cgtaggtcag taccccttcg ccccctaccc tcgtgacgcg gctgcccttc ggcaccagct
     1141 cgagatagag cccgggatcc gcaccttcgc gcgccggttc cttgccgagc accatcacct
     1201 ggcctgcccg attccagtcc aggacgcaga tcgcctcggc gtcggatccg gccgttccat
     1261 acagggcgac gtcgtagacg ccgtcaccgt cgaaatcgcc gatcaccgcg gccggcgtct
     1321 gatcgtggcg gtagcggtac gcggcgcgca tcgactcgga atagtcacgg tccggcgcct
     1381 tgcggaaggg cgacgtcagc cgggccaggc ggtccgcgga atcgggcggc gcgacgtcgt
     1441 acgcgccgta gccctccgag acgaggcgcg gggggcggag cgcgcgggag tagagccggg
     1501 gcgtcttctc ggcgtcgcgg ccggcgcgga cgaagcgctg caccgccgcg tcggcgtggc
     1561 cggttcccgg acgggccgga tagaggagca gcgtatcgcg gccctgtagc gccgcggtgt
     1621 aggcgtcata acgcggctcc catcccgtga tcgtgaggga cgcgaacgcg gcgccgcccg
     1681 ggcttgcggc agcacccgcg acgggtcgat tggggtcggt ccggagtcgg cccgcgcagc
     1741 gggcataggc gctggccgag tcccgttcga tcaacgtgta ggagccgttg gatcggatcg
     1801 tcagctgaag gccgtagccg gaggaacgcg gagtctggcc tgccccctgg gcgcgatagc
     1861 tcgcatccca gttccaggtg ccctgcagat caagagagtt gcggctcgcc ccgctcgcga
     1921 ggcaaggcgc cgaagagaga gcaagacccg ccaccgcgag cagggccagc atcgaacgtc
     1981 gcatgattcc gacctcgaaa agcccggctg ttggggacaa tcggtttcag attactcctt
     2041 cacggccttc gtgctacaag aaagggcaat gtggttccat aggtccatga agatacgatc
     2101 gctggtcctg ctcgcgctcc caaccgtgct cctgctcgcg ggttgtggtg gcgactccaa
     2161 gaatcccacc aaacccgccg gcccgacccg cagctacttc atgggcttct ccagcttccc
     2221 accgcgcgcg agcatcgcct ccatcgtggc caccatcgac gccttcgcgc cgcacgcgga
     2281 cgccgcgttg atcgtgaccg agccgccgtg ggattcgctg ctcgcgggca aggctcccga
     2341 ttcgttgatc cggaacaacc aggtggggct cacgaattat ttccgttcga aggggctcac
     2401 gaggatcgtc gtgagtgtcg atccgacgaa cgggctcgac cgctcggccg actcggcccc
     2461 cctggtcgcc gccggccgct cgctgaccga gcccgcgatc caggcgctct tccgtagcta
     2521 cgtggtcgcg atggacacgc tgatccatcc gcagtacctg agcgtggcgt cggagaccaa
     2581 cctgatccgc gccgcggcgc ccgccccgct gtaccaagcc gtcaaacagg tcgcgaacga
     2641 tgcgtacacc gatctacgcg ctcacgacgc gaacgtgaag atcttcacga cggtgcaggt
     2701 ggaggtcgct tggggacggt tgggaggctc agggccctac gtggggatca acgccgaccg
     2761 cgccgacttc ccgtttgccc aggtgattgg actttcttcc tatccatatc tcgccggatt
     2821 caccgacccg gacagccttc catccgacta ctactcgcga atcacgtccg ggaccgggct
     2881 gccggagatg gtgatcgagg gcggatggtc cagcgattcg acgacctcac ccaactggac
     2941 cgttctgaag cagaggcgct tcatcgcgcg cgaggcaacc ctactcgacc aggcgagcgt
     3001 gatcggttgg ttccagatca cgttcaccga cctggatctc gcgtcgctgg gattccccgc
     3061 gagcgccgca cccttcgcat cgctgggcct ggtggacgtg gacctgaacc caaagcccgc
     3121 gctctccgcg tgggacgcgg cgcggagcag gaacctgaag ccgccaggag tcctctaagg
     3181 attcacttcg cgagttccat tcgcgcgaac gaacccccgc tccactcgta cacggctttg
     3241 tggccgtctt cgtaggtcgc taggacggcg tcccggggca agagatgttc ccgcccgccc
     3301 ggaagcgaaa cggaactgcc tcgaagtaac ctctgaagat agagtcgcgg gcgatccgcc
     3361 tgagggaagg tatcgagcgt cgctacactt tgcgctcgat tccagtcgaa gacgcagacg
     3421 atctcctcga ccgctccgtc gttcacgcca tatcgcacca cgtccaacgt ccgatcgtcg
     3481 tcaaagaatc ccatcactgc cgatggagcc tggtcatgcc ggtatggata ggtggagcgg
     3541 accgcttccg gataatcctg ccgctggtgc agtccaaagg tcattcggta catcttcgcg
     3601 aacgaatcgg gaaactcgaa agcgtagccg taccccgacg atacgaggcg cggtggtcgc
     3661 tccgatcgag gacggggatg cagggaacca tcggacgtgg ccgccttgcg gatgtatcgg
     3721 tccgtgagcg cgtctgtgag tgagccaccc ggccaacccg gatagaggta cagcgtatcc
     3781 tggccacgaa tggccacgag aaagctcgcc tgctggttgg atctttgcgc ccagaggtcg
     3841 agcgcccagc cttccggttg caggtag
//
LOCUS       DASSLZ010000006        22358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_509123, whole genome shotgun sequence.
ACCESSION   DASSLZ010000006 DASSLZ010000000
VERSION     DASSLZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 22358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22358
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509123"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VFM00_00115"
     CDS             complement(<1..1299)
                     /locus_tag="VFM00_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HET7496681.1"
                     /translation="MKTNDASDFRAVVIGAGASGVLAAVHIARAGAHRHVALVEAGTR
                     TARGLAYGTPYGAHLLNVPARRMSAFPDRPDHFAVWLMAHVPGSGPETYAPRPLYGEY
                     LAHVLNEARDSGASVTPVPGTAVDLSRADPGWRVHLDDGRSLGGAAVVLALGNLAPGD
                     PPELAAFRDDRYVRDPWAPGVAAGLDREAPVLTVGTGLTMVDLVLALRAEGHRGPVHA
                     LSRHGRLPQEHTAYPVQAIPEPPALSTPAAAARWIRLAIAGRLESDRHRPSGHPDWRA
                     VVDSLRPHTARIWSGWSLRRRASFLRHARSLWDVHRHRIPPEVAARVRALVDAGELTI
                     HAGRITGIEARDEGLRVRWRRRGGAEERSLAVARVINATGPASNYATLDVPLVARMRR
                     AGDLVPDALGLGIETRGDGALLGRHGNPVPGLYPLGPLRRP"
     gene            1533..3134
                     /locus_tag="VFM00_00120"
     CDS             1533..3134
                     /locus_tag="VFM00_00120"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HET7496682.1"
                     /translation="MPMLIGVPAETEAGEKRVATVPEVVEKLVKLGFAVAVQSGAGAA
                     ANFDDDAYREAGAEVIDGAAALWAKADLVFKVRPPTFEEVGLMRVGATLVSFIWPAQN
                     PDLMKRLAEKKATVLAMDSVPRISRAQKLDALSSMANIAGYRAVIEAAHAFGRFFTGQ
                     ITAAGKVPPARVFVIGAGVAGLSAIGTASGLGAIVRANDTRPEVADQVVSMGGEFVPV
                     DYQEEGSGVGGYAKVMSEGFQKAQREVFAKEIKNADIVVTTALIPGKPAPRLITAAMV
                     QSMKAGSVIADMAAEQGGNCELTVPGQVVERHHVTIIGYTDLASRLSKQASTLYATNL
                     FRLTEELCKGKDGVINVDMDDEVIRGTTVIKDGAITWPPPAPKLSAAPVAAAKPAAVA
                     KPAETAKGHGHGAAEPASPVRTAILFLAAAALFWFIGAKAPAAFLPHFTVFVLACFVG
                     YMVVWNVTPALHTPLMSVTNAISSIIAIGALVQIAPPLMNGGAGVVRPASWILGLAVV
                     SLTLVSINMFGGFAVTRRMLEMFRK"
     gene            3145..4557
                     /gene="pntB"
                     /locus_tag="VFM00_00125"
     CDS             3145..4557
                     /gene="pntB"
                     /locus_tag="VFM00_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HET7496683.1"
                     /translation="MSASLATVAYIAATILFILSLGGLSNPETSRRGNLFGIIGMTLA
                     VLATIFGPQVTPAGYAWIIGSMAVGATIGLFAARIVRMTQMPELVALMHSLVGIAAVL
                     VGYANYIDPAGAGQFTGSEKTIHEIEIYLGILIGAVTFSGSLIAFGKLAGKISGKPVI
                     LPGRHLLNLAGLLAVIWFGGSFIRAHDVTQGLTPLFVMTSIALLFGVHMVMAIGGADM
                     PVVVSMLNSYSGWAAAATGFMLSNDLLIVTGALVGSSGAILSYIMCRAMNRKFLSVIA
                     GGFGTEGSAKPAAGAQPAGEVVPINATDTAELLREAKNVIIVPGYGMAVAQAQHTVYE
                     ITKYLRDKGVNVRFGIHPVAGRMPGHMNVLLAEAKVPYDIVLEMDELNGDFATTDVAM
                     VIGANDIVNPAAEDDPTSPIAGMPVLEVWKAKTSIVMKRSMASGYAGVDNPLFYKDNN
                     RMLFGDAKKTLDEVLTALKN"
     gene            complement(4573..5001)
                     /locus_tag="VFM00_00130"
     CDS             complement(4573..5001)
                     /locus_tag="VFM00_00130"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496684.1"
                     /translation="MADWLQRLARGEDDRPVEPNRASKSSSSECTYSEDLLDRERIRA
                     EIAGMARDNALWLGRKGIAARTVTIKVRYDDFTTVTRSHSEAATSDPDAIVRRAVALL
                     EKTEAGRRPVRLLGAGVYNLEAPGGADEAERSEAAQLRFD"
     gene            complement(5107..5651)
                     /gene="dinB"
                     /locus_tag="VFM00_00135"
                     /pseudo
     CDS             complement(5107..5651)
                     /gene="dinB"
                     /locus_tag="VFM00_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
     gene            complement(5670..6722)
                     /locus_tag="VFM00_00140"
     CDS             complement(5670..6722)
                     /locus_tag="VFM00_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016874933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HET7496685.1"
                     /translation="MNHARIIVTQYGGPETLRVVEEVCPEPKRGEARVRVLAAGVSLP
                     DVMAREGIHPETPAPPFTPGWDLVGVVDRLGEDVSGIEPGEVVAALPIHGAYAEFVCL
                     PSSELIPVPSGLDPAEAVSLVLNYITAYQMMHRSVTVRPGQRALIHGAAGGVGTALLQ
                     LGRLAGLEMYGTCSPRSAPAVSELGGVPIDYKAQDFLVEIRRLTGDGVDVVFDPIGGA
                     HLWRSRKALRPGGTVVGYGLRSSLRGEGLTSSRPGRRQRFRGSALFGVYIAGGWLLPG
                     RKRIVPYSIQTLKRLKPGWFRQDLTSLFDMLRTKKIKPLIAQRFPLAEARRAQELLEK
                     GGVTGKIVLVLARSDG"
     gene            complement(6832..7296)
                     /locus_tag="VFM00_00145"
     CDS             complement(6832..7296)
                     /locus_tag="VFM00_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018977094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7496686.1"
                     /translation="MSLLIREDDLRGPEIAELLRAHLAHAARHSPPESIHALDLDRLR
                     SPGITFWSAWADGALVGCGALLALAPDHGEIKSMHTAEAHRGKGVAARLLAHILDEAR
                     RRSYRRVSLETGSMAGFAPARALYARFGFQECPPFASYREDPHSVFLTLELP"
     gene            7402..8415
                     /locus_tag="VFM00_00150"
     CDS             7402..8415
                     /locus_tag="VFM00_00150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7496687.1"
                     /translation="MQYTTLGRTGLTVSRLCLGCMSYGAPSWRPWVLDEEAARPFFQR
                     AIELGINFFDTSDMYSLGVSEEVTGRALRDYARLDEVVIATKVFYPSSRPAAWWNPTV
                     EGGAPNSWGLGRKHVVQACEASLRRLGVDTIDLYQLHRVDPATPVEETLGALDLLVRQ
                     GKVRYIGCSSTWAWTLMKALAISDSRGLARFVSMQNHYNLVYREEEREMLPLCLDQGL
                     GVIPWSPLARGLLARGAGEPTPRVSGDATLAGQLYDHPGDAEVVEANRRVAAARGVTP
                     AETALAWLLSRPGVTAPIVGATRMEHLEAAARALDLVLTPEEIAALEAPYRPHAVRGW
                     IRA"
     gene            8412..8918
                     /locus_tag="VFM00_00155"
     CDS             8412..8918
                     /locus_tag="VFM00_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7496688.1"
                     /translation="MSPDPEPAVRIRPAVASDLAALGRCGAALMRQHHASDPRRFILT
                     QHPEEGYGRFLVSQLVEPDCLVRVAERSGEVVGYVFASIEPTSWRDLRGPGGFVHDVY
                     VDERARRAGTGEALLRAAIAWIHSRGMTQVVLWTKSGNESARRLFARIGFRETMHEMT
                     LDGDPSGA"
     gene            8947..9519
                     /locus_tag="VFM00_00160"
     CDS             8947..9519
                     /locus_tag="VFM00_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496689.1"
                     /translation="MHQAIAPSLEAWASFYVIVGSSAGALTGLQFVVMTLISASGRTG
                     GLETISAFGSPNVVHFCASLLVASILSIPWRGLHTAGVAVALSGALGVVYVLIVMRRA
                     ARQRTYQPVFEDWLWHVALPILAYAVLFVAGGVLAGAVPDSLFFIGPAALLLVFIGIH
                     NAWDTVTFLTATSGQPSPPPPSGPPATPSE"
     gene            9589..10077
                     /locus_tag="VFM00_00165"
     CDS             9589..10077
                     /locus_tag="VFM00_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496690.1"
                     /translation="MSERDETRAEITDEEKRANVIRLVFGDDPARFEEFCREVEAILP
                     PDASAVIRGSSVTGERFEDGAPFDADGPGTSDVDLTLVGDSVLQWYTPDGFYIPGAHS
                     KPLSDEHPDIAPDLVPLREKLSRMVGRPVNIQGTKNWTMFVKEVLLAEPCLTVIGKLE
                     RA"
     gene            10074..10817
                     /locus_tag="VFM00_00170"
     CDS             10074..10817
                     /locus_tag="VFM00_00170"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7496691.1"
                     /translation="MKLRLLSYNIRYGGVGREEGLAAVIHGAEPDLVLLQEASRPDVV
                     KRLADRTGMAYCGSVAGGSVGYLSRVELLGHEWRQPRGCSRSLLRLDLAAPECSVFAL
                     HLQAMHSNWSERRRVRELKAILSDIASHRDRFHLLAGDFNTLAPGEKLDRRRLPLRIR
                     LITSLLGSTVRWETIQIMLDAGYADAFRALHPDDSGVTFPTWDPHLRLDYLFVPAATV
                     SRVERCEVVRGGEAASASDHFPLLTVVTP"
     gene            complement(10821..11363)
                     /locus_tag="VFM00_00175"
     CDS             complement(10821..11363)
                     /locus_tag="VFM00_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014649460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7496692.1"
                     /translation="MTDAYLRDVIRTYRNYKALGDRALAQTPEAHWHTELDANSNSIA
                     VIVKHVAGNLRSRFTDFLTADGEKPDRDRDGEFTMNERATREEILGWWNQGWAAVLSA
                     IENLRPEDLERTIHIRQEPFLVVEALNRSVTHTAYHVGQIVYLARHFAGGEWTSLSIP
                     KGMSGAHQRGDFKEKGIART"
     gene            complement(11360..12193)
                     /locus_tag="VFM00_00180"
     CDS             complement(11360..12193)
                     /locus_tag="VFM00_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HET7496693.1"
                     /translation="MTDPEIQRPSPSEKGARFRALHQGPGVFVIANAWDPGSARLLAQ
                     LGFQAVATSSAAAARALGLEDGQLGRDRSLANARSIVDACDLPVSADLEDGFGEAPAV
                     AAETIRMAGAAGLAGASIEDAPAGAGVDELSLATERIAAAVEAARSLSVPFTLTARCE
                     NFVRGRPDLDDTIERLRAYERAGADVLFAPGLPTLDAVRTVCSAVAKPVNFMAGIPGK
                     SFSLPELEAAGVRRVSLAASLHRAAMAALRDAAEEVLNRGTFTYIDRLAALAATWERA
                     R"
     gene            12282..13064
                     /locus_tag="VFM00_00185"
     CDS             12282..13064
                     /locus_tag="VFM00_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496694.1"
                     /translation="MSAFPASKLEKTLGKPPGEWTAEDLARIVDEEDIGLVTLMHVGG
                     DGVLKALDFVPQTRAHLLGILRHGERADGSSLFDGLPSGASDIRLKPRIATAFLSPFS
                     PVPTLALLCGHCDREGRPLPQSPDTIVRRAFDRLRDEAGLEYWALGEVEYFLGRPAND
                     VEIERRRDRGYHATSPFVAGEGMRRRALRHLADMGVAVKYAHAEVGLVDERRTGAASG
                     SSTRSSWDWRRFRRPPTRSFSPAGPCSISRTKKGSPAVSIPS"
     gene            12959..13651
                     /locus_tag="VFM00_00190"
     CDS             12959..13651
                     /locus_tag="VFM00_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496695.1"
                     /translation="MAPLPAAADAIVLTRWALLHLAHEEGLPCRFDPILKEGHAGSGL
                     HVHSLPRSLREVPPAAAFLEGGCLGPSGRWLIGGLVRLAPALMAFGNREASSFVRLLQ
                     AKETPVAITWGAFNRKALIRIPLFAPGDACDAWAPTIEFRLPDGSAHAHLLLAGMAQA
                     LALGRGLRDLDALLERTAAHGEAIDVTRMPGLPRDREGVLAALAEARASLEAGGVFPP
                     SVLDAWLPAAVR"
     gene            complement(13648..14640)
                     /locus_tag="VFM00_00195"
     CDS             complement(13648..14640)
                     /locus_tag="VFM00_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HET7496696.1"
                     /translation="MKVLFVNGDGVARLLTMEACIPAMRDALTALSRGDVVMPLRSLV
                     RLPDGSGLLGMMPGYLGAPRSFGLKVVTVMPGNHGTAYDSHQGVVMLFGIEHGEPLAI
                     LDASAITAIRTAAASAAATDALAREDAGDLALIGSGAQAKTHLAAMHAVRPLRRVRLW
                     SRSRANAERFARAEAGGAGVPIEVLGSAEEAVRGADLVCTTTASKEPVLKGAWLSPGV
                     HVNAVGSCFPTSRELDTDAVRRARFYTDCRESCVNEAGEFLIPRDEGAITDAHLLGEL
                     GEVLLGKVEGRAARDDVTVYESLGVAVEDLAAAHVIHRRAMETGEGTWLDWGGV"
     gene            complement(14637..15608)
                     /locus_tag="VFM00_00200"
     CDS             complement(14637..15608)
                     /locus_tag="VFM00_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HET7496697.1"
                     /translation="MSSLIPIHAIRDAAARTADRLHRTPVLTATTLGERAGARLHLKC
                     ESFQKTGSFKPRGALNVVLSMEPERRARGLITVSAGNHAQAVAWAARAAGVPCTVVMP
                     ADAPRSKTDATRGYGATIVPHPDRTTLFDRMREEEARTGAAFVHPFDDPVGLAGAGTL
                     GLEIVEQVPDVACVVVPIGGGGLMAGVTSAVKALKPSCRVIGVELDASRGMTSSLSAA
                     KPIPIPVPKTIADGLTPPFVGSIPLSIAREALDEIVPITEPEIVEGMRWLLTRAKLYV
                     EGGGAAATAALLSGKVRVPAGSVVVSVVSGGNVDPERALAAIAGGDG"
     gene            complement(15611..16099)
                     /locus_tag="VFM00_00205"
     CDS             complement(15611..16099)
                     /locus_tag="VFM00_00205"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HET7496698.1"
                     /translation="MPVKPNPLPPPAPDLGAAILGIWRLTSREDYDAGGRRLIDPVLG
                     AEPLGIVCFAPKQFAAQFMNRSRGLDSWSQTAGMAGPAAGVNNTGAVDGYDAYFGRYE
                     LDLAAGTIIVSLEGALSRGNIGMKATRDVRVAGDRLWIRLATNAADGTPITRTLTFER
                     SG"
     gene            16356..16943
                     /locus_tag="VFM00_00210"
     CDS             16356..16943
                     /locus_tag="VFM00_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496699.1"
                     /translation="MRSRWEEKYTEPTDEPVTLTESQLRALKRGATIGMVAAILAIIA
                     VGIAGYSLVSARSINSDAAIASKSESTAQPAPEAAAPAASAPTTAPAVQPAAPAAQPS
                     AQPAAAPVEAKPALETQTPKAARVSAHKVTARTHRISRSIEEKPVTIPAAAPEPTPAA
                     ATAKAPALSPIAPQPVGADAPAKPKPAAHDSSAAH"
     gene            complement(16951..17211)
                     /locus_tag="VFM00_00215"
     CDS             complement(16951..17211)
                     /locus_tag="VFM00_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496700.1"
                     /translation="MSFRRITLHSARLASAMLQELFASVTRQTKAAVAEALEETRGRV
                     DLSEFRVRLAGRSPVRRNLFGVYVQGPAVASAYQDAKAHSLR"
     gene            complement(17289..19370)
                     /locus_tag="VFM00_00220"
     CDS             complement(17289..19370)
                     /locus_tag="VFM00_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7496701.1"
                     /translation="MAKALALLLLLLPATCRAARAPGAPGDSVVVLPPVVVTPTRAAE
                     DPLLVPAAVDRVDSAALARARPRLNLAESLPRIPGVLARDRQNEAQDLQISIRGFGAR
                     STFGVRGVRLYADGIPATMPDGQGQVSHFLLDAADRIEVLRGPFSALYGNASGGVIQL
                     FSADPPPRPEWRAASFAGSDRLTRGSFGVRGPWEGMRGGYLVDVAGVSEDGFREHSAS
                     RRTSAQAILRGGLGSGGSWLFALNGLDAHAEDPQGLTASDLARDRRAASPGALAFDTR
                     KTTRQGQAGGRVERKGLGSLFSVGGYAGSRAIVQFLAVPVAAQSNPLHGGGVVDLDRG
                     YYGFDARARREAVLGVGGLALTAGVERQVSNERRRGYENFVGSRLGLTGALRRDEDDR
                     VGSTDEFVQAEWALGDRWRADAGARHSEIRFRSRDHYVTAQNPDDSGALAYRRTTPVA
                     GVLWRANPRLSLYANAGKGFETPTLNELAYRPDSTSGLNTALRPARSDDVEAGVRGRA
                     ASGSYGLCGFLARTDDELVVASSLGGRTTYANAARTLRRGVEASWNGALAPRWRASLA
                     YTWLDARFRRAFGSVAEGNRIPGIPRHDVFAELRWLPCAALDLAAAVSAVDRVATNDA
                     NSESAPGYATVDLSGEARRRLGGALVTAFVRVTNALDRAAIGSVIVNEANGRYFEPAP
                     GRGWVAGITIR"
     gene            complement(19407..20696)
                     /locus_tag="VFM00_00225"
     CDS             complement(19407..20696)
                     /locus_tag="VFM00_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7496702.1"
                     /translation="MRRSDRWFHIVALLTVAMFGLSLAVVFPRAPGSPGDGRDAELRA
                     TPFPVQTRHALGRGEALSTVLAARGLDDAQTKAIIRLLKNYKSPRSLRPGMTVGVAFD
                     PVGPFGPQRTPDQVRITLSPDSVLEFTRSGASWSSQLDLVPFVVDTVCISGVITSSLW
                     SAKLGGDLDRMGEGEFEELVYDLADIFAWEVDFTRDLRKGDVFRVALERKVRPDGSVR
                     SRHFLAIELRNQDRVLRAIPVPRGARWAYFDDEGRSLHGAFLRYPVPYRITSRFTSRR
                     FHPILKTWRKHEGIDYGAPFGAPVEATASGVVTRAGFAGGYGRLVELRHAGGIRTRYA
                     HLSAIGAGIRPGARVEQGAIVGRVGASGLATGPHLHYEFVLNGRHVDPLTVELPSQPS
                     LSGTRLAEFRKVRDGALSLLEGVPIPLDIRVALSRRQ"
     gene            20843..21268
                     /locus_tag="VFM00_00230"
     CDS             20843..21268
                     /locus_tag="VFM00_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7496703.1"
                     /translation="MKNRFAGATHALLRMVAGLLFIHPGGMKLFGWFGGMPAGVTLTP
                     RLVAAGWIEIVCGTLILIGLLTRPAAFLASGEMAFAYFIGHFPHGFWPIQNHGEPAVL
                     FCFLFLFFAANGAGPASLDALIRKRRDRVDSGPSHSSYR"
     gene            21343..21759
                     /locus_tag="VFM00_00235"
     CDS             21343..21759
                     /locus_tag="VFM00_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7496704.1"
                     /translation="MRFMVIVKADKNSEAGILPDKEVFAKMGKFNEELVNAGVMLAGE
                     GLQPSSKGVRIRFSGGKKTVIDGPFAEAKELIAGFWLWQVKSKEEAIEWLKRAPFEDT
                     EVEIRQVYESEDFGDAFPQELREQEENLRGRISSRK"
     gene            22015..>22358
                     /locus_tag="VFM00_00240"
     CDS             22015..>22358
                     /locus_tag="VFM00_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7496705.1"
                     /translation="MFQRKFWGTLLLSIPTLVWAPMIQRWFRYEAPGGPAASRWISAA
                     FGTAVFLYGGIVFLQGARGELADRRPGMMTLISLAISVAFLFSLFVTFGFPGMDLWWE
                     LATLVTIMVLGH"
BASE COUNT         3343 a         7964 c         7783 g         3268 t
ORIGIN      
        1 cggccgccgc agcgggccga gcgggtagag gccagggacg ggatttccgt ggcgcccgag
       61 aagcgccccg tccccgcgcg tctcgatgcc gagtccaagc gcatccggca cgaggtcccc
      121 cgcgcgacgc atccgcgcca ccagcggaac gtccagcgtg gcgtaattcg aggcggggcc
      181 ggtggcattg atcacacgcg ccacggccag cgaccgttcc tcggcgccgc cgcgacgccg
      241 ccatcgcacc cgcagcccct cgtcacgcgc ctcgattccg gtgatccggc cggcatggat
      301 cgtcagctcc cccgcatcca ccagcgcgcg cacgcgcgcg gccacttccg gcgggatccg
      361 gtgccggtgg acgtcccaga gagaccgggc atgccgaagg aacgatgcgc gccgccgaag
      421 gctccagccg ctccagatac gggcggtgtg cggcctcagg ctgtcgacca ccgcgcgcca
      481 gtccgggtgc cccgagggac ggtgacgatc ggattcgagc ctgccggcga tcgcgagccg
      541 tatccatcgg gccgctgccg ccggggtcga aagcgcgggc ggctcgggga tggcctgcac
      601 cgggtacgcg gtgtgctcct gcgggagacg gccgtgccgc gagagcgcgt ggacggggcc
      661 tcgatgcccc tcggcgcgaa gcgccaggac cagatcgacc atggtgaggc ccgtccccac
      721 cgtcaggacg ggtgcttcgc ggtcgagccc ggcggccacg ccgggcgccc agggatcgcg
      781 gacgtaacgg tcgtcgcgga acgcggccaa ttccgggggg tcgcccggcg ccaggttccc
      841 cagcgccaga acgacggcgg cgcctccaag cgagcggccg tcgtcgagat gaaccctcca
      901 ccccgggtcc gcacgggaga ggtcgaccgc ggtccccggc accggagtga cgctcgcccc
      961 ggagtcgcgt gcctcgttaa ggacgtgcgc caggtactct ccgtagaggg ggcgcggcgc
     1021 gtacgtctcg ggaccgctgc cgggaacgtg cgccatcagc cagaccgcga agtggtcggg
     1081 acggtccgga aacgcgctca tccttcgcgc gggaacgttc aacagatgcg caccgtacgg
     1141 cgtgccgtac gcgagcccgc gcgcggtgcg cgtccccgcc tcgaccagcg cgacgtggcg
     1201 atgggcaccg gcgcgtgcga tgtggacggc cgccagcacg ccgctcgcac cggcgccgat
     1261 gacgacggcg cggaagtcgg aggcgtcgtt ggtcttcacg agacgggggc cgggcccatg
     1321 agcgcgcgat ggtagacggc tggtggcccg tgcgcaatcg aaccgccggc cccatgggac
     1381 ctaagtaggt gcatgagacc cccttccggc tatcctcgcg cgccttgttg ggaacccgcc
     1441 gatccggcat gatgcgggta cgtgacagcg gttccgaagc gcggtcgaac gacgcgcccc
     1501 accgggacgc aagtacccta cagggagcct tcatgccaat gctgattggt gttccggccg
     1561 aaacggaggc cggcgagaag cgcgtcgcca cggtgcccga ggtcgtcgag aagctggtca
     1621 agctcggctt cgcggtggcc gtgcagagcg gcgccggcgc ggccgctaac ttcgacgacg
     1681 acgcctaccg cgaggcgggc gccgaagtga tcgacggcgc ggcggcgctc tgggccaagg
     1741 cggacctggt cttcaaggtg cgccctccga cgttcgagga ggtcggcctg atgcgggtgg
     1801 gggccaccct ggtctccttc atctggcccg cgcagaaccc cgatctcatg aagcgcctgg
     1861 ccgagaagaa ggccaccgtg ctcgcgatgg acagcgttcc ccggatctcc cgcgcgcaga
     1921 agctggacgc tctctcctcc atggccaaca tcgcgggcta ccgggccgtg atcgaggcgg
     1981 cgcacgcctt cggccgcttc ttcaccggac agatcaccgc cgccggcaag gtgcctcccg
     2041 cccgggtgtt cgtcatcggc gccggcgtcg cggggctgtc ggccatcggc accgcgtcgg
     2101 gtctcggcgc gatcgtccgc gccaacgaca cgcgccccga ggtggccgac caggtcgtct
     2161 cgatgggcgg cgagttcgtt ccggtggact accaggaaga gggctcgggc gtcggcggct
     2221 acgccaaggt catgagcgag ggcttccaga aggcgcagcg cgaggtcttc gccaaggaga
     2281 tcaagaacgc ggacatcgtc gtcacgacgg ccctcatccc cggcaaaccg gcgccgcgcc
     2341 tgatcacggc ggcgatggtg cagtccatga aagcgggaag cgtcatcgcg gacatggccg
     2401 ccgaacaggg cggcaactgc gagctgaccg tgccgggcca ggtggtggag cggcatcacg
     2461 tgaccatcat cgggtacacc gatctcgcca gccgcctgtc caagcaggcg agcacgctct
     2521 acgcgacgaa cctcttccgg ctgaccgagg agctgtgcaa gggcaaggac ggcgtcatca
     2581 atgtcgacat ggacgacgag gtcatccggg gcacgacggt gatcaaggac ggcgcgatca
     2641 cctggcctcc gcccgcgccc aagctctccg cggcgccggt cgccgcggcg aagccggcgg
     2701 cggtggcgaa gccggcggaa acggcgaagg gccacggcca tggcgcggcc gagccggcct
     2761 cgccggtgcg caccgcgatc ctgttcctgg cggcggccgc cctgttctgg ttcatcggcg
     2821 cgaaggcgcc ggccgccttc ctgccccatt tcaccgtttt cgtgctggcc tgcttcgtgg
     2881 ggtacatggt ggtctggaac gtcacccccg ccctgcatac gccgctcatg agcgtcacga
     2941 acgcgatctc cagcatcatc gcgatcggcg ccctggtgca gatcgccccg ccgctgatga
     3001 acgggggggc cggcgtcgtg cgtccggcat cctggatcct cgggctcgcg gtggtgagtc
     3061 tcacgctcgt ctcgatcaac atgttcggcg gattcgcggt cacgcgccgc atgttggaaa
     3121 tgttccggaa ataggggggc cctcgtgtct gcgagtctcg caaccgtcgc ctatatcgcc
     3181 gcaacgatcc tgttcatcct gagcctcggc ggactctcca atccggagac gtcccgccgc
     3241 ggaaacctgt tcggcatcat cggcatgacc ctcgccgtcc tggccacgat cttcgggccg
     3301 caggtgactc ctgccggcta cgcgtggatc atcggctcga tggccgtcgg cgccacgatc
     3361 gggctcttcg ccgcgcgcat cgtgcggatg acacagatgc ccgagctggt cgccctgatg
     3421 cacagcctcg tggggatcgc ggccgtgctc gtgggctacg ccaactacat cgaccccgcg
     3481 ggcgccgggc agttcacggg ctccgagaag acgatccacg agatcgagat ctatctcggc
     3541 atcctgatcg gcgccgtcac cttctcagga tcgttgatcg cgttcggcaa gctggccggc
     3601 aagatcagcg gcaagccggt catcctgccc gggcgtcacc tcctcaacct ggccggcctc
     3661 ctggcggtga tctggttcgg cggctccttc atccgcgcgc acgacgtgac gcagggcctg
     3721 accccgctct tcgtgatgac ctccatcgcg ctgctcttcg gcgttcacat ggtcatggcc
     3781 atcggcggcg ccgacatgcc ggtggtggtg tcgatgctca acagctattc cggctgggcc
     3841 gcggcggcga ccggcttcat gctgtcgaac gatctcctga tcgtgaccgg tgctctggtg
     3901 ggctcgagcg gcgccatcct ctcctacatc atgtgccgcg ccatgaaccg gaagttcctc
     3961 tccgtgatcg cgggcggatt cggcaccgag ggaagcgcca agcccgccgc gggcgcgcag
     4021 cctgccggcg aggtcgtgcc gatcaacgcg accgacaccg ccgagctctt acgggaagcg
     4081 aagaacgtga tcatcgtgcc ggggtacggc atggccgtgg cgcaggcgca gcacacggtc
     4141 tacgagatca cgaagtacct gcgcgacaag ggcgtcaacg tgcgcttcgg catccatccg
     4201 gtcgccggcc gcatgcccgg ccacatgaac gtgctcctgg ccgaggccaa ggttccctac
     4261 gacatcgtgc tggagatgga cgagctgaac ggagacttcg ccacgacgga cgtggccatg
     4321 gtcatcggcg ccaacgacat cgtgaacccc gcggcggagg acgacccgac cagccccatc
     4381 gccggaatgc cggtgctcga ggtctggaag gccaagacct cgatcgtcat gaagcgcagc
     4441 atggcgtcgg gctacgccgg cgtggacaat ccgctcttct acaaggacaa caaccggatg
     4501 ctgttcgggg acgccaagaa gacgctggac gaggtgctga ccgcgctcaa gaactgaccg
     4561 gcggcgaagc ggtcagtcga accgcagctg cgcggcctcg gaccgctccg cctcgtccgc
     4621 cccgcctgga gcttccaggt tgtacacccc cgccccgagc agccgcaccg gacggcggcc
     4681 ggcctcggtc ttctcgagca gcgcgacggc gcgccggacg atcgcatcgg ggtcgctcgt
     4741 cgccgcctcc gaatggctcc gggtgacggt cgtgaagtcg tcgtagcgaa ccttgatggt
     4801 cacggttcga gcggcgatcc ccttccggcc cagccacagc gcgttgtccc gcgccatccc
     4861 cgcgatctcg gcgcgaatcc gctcccgatc caggaggtcc tccgagtagg tgcattcgga
     4921 ggacgacgac ttggaggcgc gattgggctc gacgggacga tcgtcttccc cgcgggcgag
     4981 ccgctggagc cagtcggcca tgctgccgac cgcgtcgcgc agaacctcgg gatcggcggt
     5041 tcggacgtcc gtcaggcggt cgatgccccg ggcccgcagc cgcttcgccg tcacggggcc
     5101 gacgccccac agggcatcca cgggtagctg ctgcagaaag gactcgatcc gttccggagc
     5161 caccacggtg aggccgtcgg gcttgcgcca accggaagcg atcttagcca ggaacttgtt
     5221 gggcgccacg cccgcggatg cggtgagtcc ggtggtctcg cggatcacgg ccttcagccg
     5281 ccgcgccaca tcgacgccca gcggctcgcg gcgttctccg tcacgtcgag atacgcctcg
     5341 tccagcgaga gcggctcgac cagcggggtg acgccgcgca ggatggccaa aatctgctgc
     5401 gagacggacc gatacttgga gaaatcgggg gacacgatcg ccaggccggg acacaggcgg
     5461 acggcccggc tcatcggcat ggccgaatgc acgccgtgcc ggcgcgcctc gtagctcgcg
     5521 gcggcgacca cgccgcgcga atccggcgag ccgccgacgg ccaccggcac gccgcgaagc
     5581 tccggccggt cgcgctgctc gacggacgcg tagaaggcat ccatgtcgat gtggacgatg
     5641 cgtcggatca tcggatcaga ggatgggcct catccatcgc tacgcgcgag cacgaggacg
     5701 atcttgcccg tcacgccgcc cttctcgagg agctcctgcg cccgacgcgc ctcggcgagc
     5761 ggaaaccgct gcgcgatgag cggcttgatc ttcttcgtcc ggagcatgtc gaagagcgac
     5821 gtcaggtcct gtcgaaacca acccggtttc aggcgcttga gcgtctggat gctgtacggg
     5881 acgatcctct tccgcccggg aagcagccag ccgcccgcga tatatacccc gaagagggcg
     5941 cttccacgaa agcgctgacg acgtcccggg cggctggagg tcaacccctc cccacgcagg
     6001 gacgaccgga ggccgtagcc gacgaccgtg ccgccgggac gaagcgcctt gcgggaccgc
     6061 cacaggtggg cgccgccgat gggatcgaag acgacgtcca ctccatcccc cgtgaggcgg
     6121 cggatctcga ccaggaagtc ctgcgccttg taatcgatgg gaacgccccc cagctcggag
     6181 acggccgggg cgctgcgagg ggaacaggtt ccatacatct ccaggccggc caggcgcccc
     6241 agctgcagga gcgccgtgcc gacgccgccc gccgcgccat ggatcaaggc gcgctggcca
     6301 ggccggaccg tcacggagcg atgcatcatc tggtaggcgg tgatgtagtt cagcacgagg
     6361 ctgacggctt cggcggggtc cagcccggaa ggcacgggaa tcagctcgct cgagggcagg
     6421 cagacgaact ccgcatacgc gccgtgaatg ggcagcgcgg cgacgacctc gccgggctcg
     6481 attccggaga cgtcctcgcc gagccggtcg acgacgccga ccagatccca ccccggtgtg
     6541 aagggcggag cgggcgtctc gggatggatc ccctcgcggg ccatgacgtc gggcagggag
     6601 acccccgcgg ccagcacccg cacccgcgcc tcgccgcgct tcggctcggg gcacacttct
     6661 tccacgaccc ggagggtttc gggtccgccg tactgcgtga caatgatgcg tgcgtggttc
     6721 atgggcgtcc tcttcccctt ccgggtactg acgcatgcat gagctgcaac tctagtcgcg
     6781 atcagccgcc ggcgccatta ggaaccggcg aggatccgcg gtggacacgg cctagggcag
     6841 ctcgagcgtc aggaacacgc tgtgcgggtc ttcgcgatac gaggcgaagg gcgggcactc
     6901 ctggaagccg aagcgggcgt agagcgcccg cgccggcgcg aaacccgcca tcgagccggt
     6961 ctccaggctg acgcgccggt aggatcgacg cctggcctcg tccaggatgt gggccagaag
     7021 gcgcgcggcc acccccttcc cgcggtgcgc ctcggcggtg tgcatcgact tgatctcgcc
     7081 gtggtccgga gccagcgcca ggagcgcgcc gcaccccacc aacgcgccgt cggcccaagc
     7141 gctccagaag gtgatcccgg gggaacgcaa ccggtcgaga tccagcgcgt gaatgctctc
     7201 gggcggcgag tgtctcgcgg cgtgagcgag gtgggcccgg agcagctcgg cgatctcggg
     7261 accacgcagg tcgtcctcgc gtatgaggag gctcatgcag tgagccttgt accggaaatc
     7321 gggtgcactt gccaggggcg ctcgctagac tcccccgcca gcgcggatgg gaagcttgcc
     7381 ggcacgctcc ggaggtgcgt catccagtac acgaccctag gccgaaccgg cctcaccgtc
     7441 tcacgactct gcctcggctg catgagctac ggcgcccctt cgtggcggcc ctgggtgctg
     7501 gacgaggagg cggcccgccc cttcttccag cgcgcgatcg agctcgggat caacttcttc
     7561 gacacgtcgg acatgtactc gctgggcgtg agcgaggagg tcacgggccg cgcgctgcgc
     7621 gactatgccc ggctggacga ggtcgtgatc gcgaccaagg tgttctatcc gtcgtcccgg
     7681 ccggccgcct ggtggaatcc gacggtggaa ggaggcgctc cgaacagctg gggtctcggt
     7741 cgcaagcacg tggtgcaggc ctgcgaggcc agccttcgcc gcctcggcgt ggacacgatc
     7801 gatctctatc agctccaccg ggtcgacccc gccacgccgg tcgaggagac gctcggggcg
     7861 ctcgatctcc tggtgcgcca gggcaaggtg cgctacatcg gctgcagctc gacctgggcc
     7921 tggacgctga tgaaggcgct ggcgatctcc gattcccgcg gcctcgcgcg gttcgtctcg
     7981 atgcagaacc actacaacct ggtctaccgc gaggaggaac gggagatgct tccgctctgc
     8041 ctggaccagg gcctgggcgt gatcccatgg tcgccgctcg cgcgcggact cctcgcgcgc
     8101 ggggcgggcg aacccacgcc ccgcgtgagc ggcgacgcga ccctggccgg ccagctctac
     8161 gatcatcccg gcgacgcgga ggtggtcgag gcgaaccggc gcgtggcggc ggctcgcggc
     8221 gtcacgcccg cggagaccgc gctggcgtgg ctcctttcgc ggcccggcgt gaccgcgccg
     8281 atcgtcggag cgacgcggat ggagcacctg gaagccgcgg cgcgcgcgct cgacctggtc
     8341 ctgacgcccg aggagatcgc cgcgctcgaa gcgccgtacc ggccgcacgc ggtgcggggg
     8401 tggatccggg cgtgagtccc gatccggaac cggccgtccg gattcgaccc gccgtcgcat
     8461 ccgacctggc cgcgctcggg cgctgcggcg ccgcgctgat gcgccagcac cacgcctcgg
     8521 acccgcgccg gttcatcctc acgcagcatc cggaggaggg gtacggacgg ttcctcgtct
     8581 cccagctggt ggagcccgac tgcctcgtgc gcgtggccga gcgctcgggc gaggtcgtgg
     8641 gctacgtctt cgccagtatc gagccgacga gctggagaga tctgcgtggt cccggcgggt
     8701 tcgtccacga cgtctacgtc gacgagcgcg cgcggcgcgc cggcaccggc gaagcgctcc
     8761 tgcgcgccgc gatcgcctgg atccactcca ggggcatgac gcaggtggtg ctctggacca
     8821 agtccgggaa cgagtctgcg cggcgcctgt tcgcgagaat agggtttcga gagaccatgc
     8881 acgagatgac gctggacgga gacccttccg gcgcctgaac cggcgatcgg ccaaggaggt
     8941 tcggtcatgc accaagccat cgcgccttcc ctggaggcct gggccagctt ctacgtgatc
     9001 gtcggctctt cggcgggcgc gctgaccggg ctccagttcg tcgtcatgac gctcatcagc
     9061 gcgagcggcc ggacgggcgg cctggaaacc atctccgcct tcggctctcc gaacgtggtg
     9121 cacttctgcg cttccctgct cgtggccagc attctctcca tcccctggcg cggcctgcac
     9181 accgcgggcg tcgccgtcgc gctctcgggc gcgctcggcg tcgtctacgt gctcatcgtc
     9241 atgaggcggg cggcacggca gcgcacctac cagcccgtct tcgaagactg gctctggcac
     9301 gtcgcccttc cgattctggc ctacgccgtc ctgttcgtgg cgggcggggt tctcgcgggc
     9361 gccgttccgg actcgctctt cttcatcggc cctgccgcat tgctgctggt gttcatcgga
     9421 atccacaacg cctgggacac ggtgacgttc ctgacggcca cctcggggca gccatcgccg
     9481 ccgccgccct ccggccctcc ggccactcca agcgagtagc cggcccgaag cggctcgtcg
     9541 cgcggtagaa tcggtgacgg tggggcccga accaggagga acgaacccgt gtccgaacgt
     9601 gacgagaccc gggccgagat caccgacgag gaaaagcggg ccaacgtgat ccggctcgtc
     9661 ttcggcgacg atccggcccg tttcgaagaa ttctgcagag aagtggaagc catcctgccc
     9721 cctgacgctt cggccgtgat tcggggaagc tcggtcaccg gggagcgctt cgaggacggc
     9781 gcgccgttcg acgcggacgg gccgggcacg agcgacgtgg atctcacgct cgtcggcgac
     9841 tcggtgctgc agtggtacac gccggacggc ttctacattc cgggcgccca ctcgaagccg
     9901 ctgagcgacg agcaccccga catcgcgccg gacctggttc cgctgcggga gaagctctcc
     9961 cgcatggtgg gacggccggt gaacatccag ggcacgaaga actggaccat gttcgtgaag
    10021 gaagttctcc tggccgagcc ctgcctgacc gtgatcggca agctcgagag agcgtgaagc
    10081 tccgccttct cagctacaac atccggtacg gcggcgtcgg gcgggaagag gggcttgccg
    10141 ccgtgatcca cggggccgag cccgacctgg ttctcctgca agaggcgtcc cggcccgacg
    10201 tcgtgaagcg cctggccgac cgcaccggga tggcctattg cggctccgtc gccgggggct
    10261 cggtgggtta cctgagccgt gtggagcttc ttggccacga gtggcgccag ccccgcgggt
    10321 gctcccgttc gcttctgcga ctggatctgg cggccccgga gtgcagcgtc ttcgcgctgc
    10381 acctgcaggc gatgcacagc aactggtcgg agcggcgccg cgtgcgggag ctgaaggcca
    10441 ttctgagcga catcgcctcg catcgggacc gcttccatct cctggccggc gacttcaaca
    10501 cgctcgctcc gggggaaaag ctggatcgaa ggcgcctgcc gctgcggatc cggctgatca
    10561 cgtcgctcct cgggagcacc gtgcgctggg aaaccatcca gatcatgctc gacgcgggct
    10621 acgcggacgc cttccgcgcg ctgcatccgg atgactccgg cgtcaccttc ccgacgtggg
    10681 atccgcatct gcggctcgac tatctcttcg ttcccgccgc cacggtgagc cgggtggagc
    10741 gctgcgaggt ggtgcgtggc ggggaggccg cgagcgcctc ggatcacttc ccgctgctga
    10801 cagtggtcac gccctgacct tcaggtccgc gcgatcccct tctccttgaa gtcgccccgc
    10861 tggtgggcgc ccgacattcc cttggggatc gagagcgacg tccattcccc gcccgcgaag
    10921 tggcgcgcga ggtagacgat ctggcccacg tgatacgcgg tgtgcgtaac cgatcggttg
    10981 agcgcctcga cgacgaggaa gggctcctgc cggatgtgaa tggtgcgctc cagatcctcg
    11041 gggcgcaggt tctcgatcgc actcaagacg gccgcccagc cctggttcca ccagccgagg
    11101 atctcctcgc gggtcgcgcg ctcgttcatc gtgaactctc cgtcgcggtc ccggtcgggc
    11161 ttctcgccgt cggccgtcag gaaatccgtg aaccgggagc gcaggttgcc cgcgacgtgc
    11221 ttgacgatga cggcgatgct gttggagttg gcgtccagct cggtgtgcca gtgcgcttcg
    11281 ggcgtctggg ccagggccct gtccccgagc gccttgtagt tccggtacgt gcggatcacg
    11341 tcccgcaggt aggcgtcggt catcgcgctc tctcccacgt cgcggccaaa gccgcgagcc
    11401 ggtcgatgta ggtgaacgtg ccccgattca ggacctcctc ggcggcatcg cgcagggccg
    11461 ccatggcggc gcggtgcagc gaggccgcga ggctcacccg gcgcacgccg gccgcttcga
    11521 gctcggggag ggaaaaggat ttcccgggga ttcccgccat gaagttcacc ggcttcgcga
    11581 ccgcggagca gaccgtccga acggcatcga gcgtgggcaa acccggcgcg aagagcacgt
    11641 ccgcgccggc ccgctcgtac gcgcgcagcc tctcgatcgt atcgtccagg tcgggacgcc
    11701 cgcgcacgaa gttctcgcag cgcgccgtca gggtgaacgg cacgctcagc gatcgcgccg
    11761 cctcgaccgc cgctgcgatc cgctccgtcg ccaggctgag ctcatcgacg ccggcgccgg
    11821 cgggcgcgtc ttcgatcgac gcccccgcga gaccggcggc gcccgccatc cggatcgttt
    11881 cggccgccac ggccggggcc tcgccgaatc cgtcctcgag gtccgccgag accggaagat
    11941 cgcaggcgtc cacgatcgat cgcgcgttgg ccagggagcg gtcccgtccc agctggccgt
    12001 cctccagccc cagcgccctc gcggccgcgg cgctggaggt ggccacggcc tggaacccca
    12061 gctgggcgag aagccgcgcc gatccaggat cccaggcgtt cgcgatcacg aacacgcccg
    12121 ggccctgatg cagcgcccgg aagcgggcgc ccttctccga cggcgaggga cgctggatct
    12181 cgggatcggt catggggtcc tcatgatacg ccttggcacc ccccgcctcc ggtacctaag
    12241 tcgccctgct cggccggacc ggcacgccgt acgatgatgg ggtgagcgcg tttcccgctt
    12301 cgaagctgga gaagaccctg ggcaagccgc cgggcgaatg gacggcggag gatctcgcgc
    12361 gcatcgtcga cgaagaggat atcggcctcg tcacgctgat gcacgtcggc ggggacggcg
    12421 tcctgaaggc gctcgacttc gtgcctcaga cccgcgcgca tctcctcggc atcctgcgcc
    12481 atggcgagcg cgccgacggg tccagcctgt tcgacgggct cccgagcggc gcgagcgaca
    12541 ttcgcctgaa gccccggatc gcgaccgcgt tcctgagccc gttctccccg gttcccaccc
    12601 tggccctcct ctgcgggcat tgcgatcgcg aggggcggcc gctgccgcag tcccccgaca
    12661 ccatcgtgcg ccgcgcgttc gatcgccttc gcgacgaggc cggcctggag tactgggccc
    12721 tgggcgaggt ggagtacttc ctgggccgcc ccgcgaacga cgtggagatc gagcgccggc
    12781 gcgaccgcgg ctatcacgcg acgtccccgt tcgtggcggg cgaggggatg cgccgccgcg
    12841 cgctgcgcca tctggcggac atgggcgtcg ccgtgaagta tgcgcacgcc gaagtggggc
    12901 tcgtcgacga gcgaaggacc ggggccgcat ctgggagcag cacgagatcg agctgggatt
    12961 ggcgccgctt ccggcggccg ccgacgcgat cgttctcacc cgctgggccc tgctccatct
    13021 cgcgcacgaa gaagggctcc cctgccgttt cgatcccatc ctgaaggagg ggcatgccgg
    13081 gagcggactg cacgtccaca gcctgccccg ttccctgcgt gaggtcccgc cggcggcggc
    13141 attcctcgaa ggaggatgcc tgggaccctc gggtcgctgg ctcatcggag ggctggtgcg
    13201 tctggcgccc gcgctcatgg ccttcggcaa tcgcgaggcg tcctcgttcg tgcgcctgct
    13261 gcaggcgaaa gagaccccgg tcgcgatcac ctggggagcc ttcaaccgga aggcgctgat
    13321 ccggatcccg ctcttcgccc cgggggacgc ctgcgacgca tgggcgccca ccatcgagtt
    13381 ccgtcttccc gacggatcgg cgcacgcgca tctcctgctc gccggcatgg cccaggcgct
    13441 ggccctgggg cgcgggctcc gcgacctcga cgccctcctc gagcgcaccg ccgcgcacgg
    13501 cgaggcgatc gacgtcacgc gcatgcccgg gctgccgcgc gatcgcgagg gggtcctggc
    13561 cgcgctggcc gaggcacgcg cttcgctgga ggcgggagga gtcttccctc cctcggtcct
    13621 ggacgcctgg cttcccgccg ccgtccgcta gaccccaccc caatcgagcc aggttccttc
    13681 gccggtctcc atcgcgcgcc ggtggatgac gtgcgccgcc gcgaggtctt cgaccgccac
    13741 gcctagcgac tcgtagacgg tgacgtcgtc gcgcgcggcg cgtccctcga ccttgccgag
    13801 aagcacctcg cccagctcgc ccagcaggtg tgcgtcggtg atggcgccct cgtcgcgggg
    13861 aatgaggaat tcgcccgcct cgttcacgca cgactcccgg cagtcggtgt agaacctcgc
    13921 ccggcgcacg gcgtccgtat cgagctcgcg gctggtcggg aagcaggagc ccacggcgtt
    13981 cacgtggacg ccgggcgaaa gccaggcgcc cttcagcacc ggctccttcg acgcggtggt
    14041 cgtgcagacg aggtccgcgc cgcgcaccgc ctcctccgcg ctcccaagga cctcgatcgg
    14101 gactccggcg ccccctgcct cggcgcgcgc gaaccgttcc gcgttcgcgc ggctccggct
    14161 ccagaggcgc acgcggcgga gcggacgcac cgcgtgcatc gccgcgagat gggtcttcgc
    14221 ctgcgctccc gatccgatca gggccaggtc ccccgcgtcc tcgcgcgcca gcgcgtcggt
    14281 ggccgccgcc gacgccgcgg cggtccggat cgccgtgatc gcgctcgcgt ccaggatcgc
    14341 gagcggctcg ccgtgctcga tcccgaacag catcaccacc ccctggtgcg agtcgtacgc
    14401 ggttccgtga ttccccggca tcaccgtgac caccttgagt ccgaagctgc gcggcgctcc
    14461 caggtagccg ggcatcattc cgagcaggcc ggagccgtcg ggcaggcgga cgaggctgcg
    14521 gagcggcatc acgacgtcgc cgcgcgacag ggcggtcaga gcgtcgcgca tcgcgggaat
    14581 gcacgcctcc atcgtgagga ggcgcgccac gccgtctcca ttcacgaaca ggaccttcac
    14641 ccgtcccctc ccgcgatcgc ggcgagcgcg cgctcgggat ccacgttccc ccccgagacg
    14701 acgctcacga cgacgctccc ggcgggaacg cgcaccttcc cggagagaag ggcggcggtg
    14761 gccgccgcac ctccaccctc cacgtagagc ttggcgcgcg tgagcagcca gcgcataccc
    14821 tcgacgatct cgggttcggt gattgggacg atctcgtcga gcgcttcgcg cgcgatcgaa
    14881 agcgggatcg atcccacgaa gggcggcgtg agcccgtcgg cgatggtctt gggaacggga
    14941 atcgggattg gcttggccgc cgataaggac gacgtcatgc cccggctcgc gtccagctcg
    15001 accccgatca cgcggcagga tggcttcagc gccttgacgg cggaggtcac gccggccatc
    15061 aagcccccgc cgccgatcgg cacgacgacg caggcgacgt cggggacctg ctccacgatc
    15121 tcgagcccga gcgtgccggc tcccgcgagc cccaccgggt cgtcgaaagg atgcacgaac
    15181 gccgccccgg tgcgcgcctc ctcctcgcgc atccggtcga acagcgtcgt tcgatcggga
    15241 tgcggcacga tcgtcgcgcc gtacccccgc gtcgcatccg tcttggaccg cggcgcgtcg
    15301 gcgggcatga cgacggtgca cggaacgccg gccgcccgcg cggcccaggc cacggcctgg
    15361 gcgtggttcc ccgccgagac cgtgatgagc ccgcgcgcgc ggcgctccgg ctccatggag
    15421 aggacgacgt tgagcgcgcc ccgtggcttg aaggagccgg tcttctggaa gctctcgcac
    15481 ttgagatgaa gccgcgcgcc ggcgcgctcc cccagagtcg tggccgtcag gaccggcgtc
    15541 cgatggagcc ggtccgcggt cctcgccgcg gcgtcgcgaa tcgcgtgaat cgggatcagg
    15601 ctcgacaccg ctatccggag cgttcgaacg tgagcgtgcg cgtgatcggc gtgccgtcgg
    15661 cggcgttcgt cgcgaggcgg atccagagcc gatcgccggc gacgcgcacg tcgcgggtcg
    15721 ctttcattcc gatgttgccc cgcgagagcg cgccctccag cgacacgatg atcgtgccgg
    15781 cggcaaggtc gagctcgtac ctgccgaagt acgcgtcgta gccgtccacg gcgccggtgt
    15841 tgttgacgcc ggcggccggt cccgccatcc ccgcggtctg ggaccatgag tccaggccgc
    15901 gcgagcgatt catgaactgc gcggcgaact gcttcggcgc gaagcacacg atgccgaggg
    15961 gctcggcccc caggaccggg tcgatcagcc ggcggccgcc cgcgtcatag tcctcgcggg
    16021 acgtgaggcg ccagattccc agaatggccg cacccaggtc gggagcgggc ggcggcaatg
    16081 gattgggctt cacgggcatt ccctctcgtg gcgcgcgggt tcgaaggcgg acagcttacg
    16141 ggagcggcct cgtcggtaca attcccgagc ccgaacgtac aacgggcccg cgtttctggc
    16201 gtctcctcca ccgggtaagc cccggacccg gccgcgtttc cccattcagc cccggacggt
    16261 tcttcctctg gcacgccact tgatccccga acgaagcttg ccgcacccgc ctggtggcgg
    16321 ccccgatccg atatcgactg ccgaaggagg gcatcatgag gagccgttgg gaagagaagt
    16381 acacggaacc gaccgatgaa ccggtcaccc tcaccgagag ccagctgcgg gccctgaagc
    16441 ggggcgcgac gatcggcatg gtcgccgcga tcctggccat catcgcggtc ggcatcgcgg
    16501 ggtacagcct cgtctcggcc cgaagcatca actccgacgc cgccatcgcc tcgaagtcgg
    16561 aatcaacggc ccagccggca cccgaagccg cggcgcctgc cgcctcggct ccgacgacgg
    16621 ccccggccgt ccaacccgcg gcaccggcag cgcagccgtc cgctcagccc gccgcggcac
    16681 ccgtcgaggc caagcccgca ctggagacgc agactccgaa ggctgcccgc gtcagcgccc
    16741 acaaagtcac ggcgcgcacg caccggatct cccggtcgat cgaggagaag ccggtgacga
    16801 ttccggcggc cgcgcccgag ccgacgccgg cggcggccac cgccaaggcg cccgcgctct
    16861 cgccgatcgc ccctcagccc gtgggcgccg atgctccggc gaagccgaag ccggccgcgc
    16921 acgattcgag cgcggcgcac tgatcggcga ctaccggaga gaatgcgcct tcgcgtcctg
    16981 ataggcggag gccacggccg ggccctgcac gtacacgccg aacagatttc ggcggaccgg
    17041 tgagcggccg gcgagacgga ctcggaattc ggacaagtcg acccggccgc gcgtctcttc
    17101 cagcgcctcc gcgacggccg ctttcgtctg gcgcgtgacc gaggcgaaca gctcctggag
    17161 catggccgac gcgaggcgcg ctgaatgcag ggtgattctt cgaaacgaca tggggctggg
    17221 tctctcctgc cggccaggaa ggatgcgggg agtgtacgcg aggcgcggca cctcgcacca
    17281 ccggggcgtc agcggatcgt gattcccgcg acccaaccgc gtcccggagc cggctcgaag
    17341 tagcgcccgt tcgcctcgtt cacgatcacc gagccgatcg ccgcgcgatc gagcgcgttc
    17401 gtcacccgga cgaacgccgt aacgagcgcg ccgcccaggc ggcggcgcgc ttccccgctc
    17461 aggtccacag tggcgtagcc gggggcggac tcggaattcg cgtcgttcgt cgcgacacga
    17521 tccacggcgc tcacggccgc cgcgagatcc agcgccgcgc agggaagcca gcgcagctcg
    17581 gcgaagacgt cgtgacgagg aatgccgggg atccggttcc cctccgccac ggagccgaag
    17641 gcgcggcgga agcgcgcatc gagccacgtg taggccaggc tcgcccgcca ccgcggcgcc
    17701 agcgctccgt tccatgacgc ctcgacgcct ctccggaggg tgcgcgccgc gttcgcgtag
    17761 gtggtgcggc cccccaggct ggaggccacg acgagctcgt cgtcggtcct cgcgagaaag
    17821 ccgcacaatc catagcttcc cgacgccgcg cgtccccgga ccccggcttc cacgtcgtcg
    17881 ctccgcgcag gacggagcgc ggtgttgagg ccgctcgtgc tgtcggggcg ataggccagc
    17941 tcgttcaggg tgggcgtctc gaaccccttt cccgcgttcg cgtacaggct gagtctcggg
    18001 ttcgcgcgcc acagcacccc cgcgaccggg gtcgtcctcc ggtacgccag ggcgccgctg
    18061 tcgtccgggt tctgcgcggt cacgtagtgg tcgcgcgatc ggaagcggat ctcgctgtgc
    18121 cgggcgccgg cgtccgcgcg ccaccgatcg ccgagcgccc attcggcctg cacgaattcg
    18181 tcggtcgagc ccacgcgatc gtcttcgtcg cggcggagcg cgccggtcag gccgagccga
    18241 gagcccacga agttctcgta gccgcgacgc cgctcgttgg agacctgccg ctcgaccccg
    18301 gcggtcagcg ccagcccgcc gacgccgagc acggcctccc ggcgcgcgcg cgcgtcgaag
    18361 ccgtaatagc cacggtccag atccaccacg ccgcccccat ggagcgggtt cgactgggcg
    18421 gccaccggca ccgcgaggaa ctgtacgatc gcccgggagc ccgcgtagcc tcccacgctg
    18481 aacagcgagc cgagcccctt tcgctcgacg cgtccccccg cctgtccctg gcgggtggtc
    18541 ttccgcgtgt cgaacgccag cgcgcccgga ctggccgcgc ggcggtcgcg ggccaggtcg
    18601 ctcgcggtga gtccctgggg atcctccgcg tgcgcgtcca gcccgttcag cgcgaagagc
    18661 catgagcctc cggagccgag cccgccgcgg aggatcgcct gcgccgaggt gcgccgcgac
    18721 gcgctgtgct cgcgaaaccc atcctccgag acccccgcga cgtccaccag gtaaccgccg
    18781 cgcatccctt cccacggccc gcgcacgccg aaggagccac gggtcaggcg gtccgatccg
    18841 gcgaacgagg ccgcgcgcca ctcgggccgc ggcggcgggt cggcgctgaa aagctggatc
    18901 acgccgccgg aggcgttccc gtagagggcc gaaaaggggc cgcgcagcac ctcgatgcga
    18961 tcggcggcgt ccagcaggaa gtgcgagacc tgcccttggc cgtccggcat cgtggcgggg
    19021 atgccgtcgg cgtagaggcg caccccgcgc acgccgaacg tggagcgcgc gccgaagccg
    19081 cggatcgaga tctggagatc ctgcgcttcg ttctgccggt cgcgcgcgag cacgccgggg
    19141 atgcgcggga gcgactccgc gaggttcagc cgtggccggg cgcgcgccag cgccgccgag
    19201 tccacacggt cgacggcggc gggcacgagc agcggatctt cggcggcgcg cgtcggggtg
    19261 acgacgacgg gagggagcac gaccacggaa tcgccgggcg cgcctggagc gcgtgcggcg
    19321 cggcaggtcg cgggcaggag caggagaagc aacgcgagcg ccttggccat cgccggtgca
    19381 ctctaccggc gcggccccgg gggcgtctat tgccgacggc tcagagccac ccggatgtcg
    19441 agggggatcg gcacgccttc caggagcgac agcgccccat cgcgcacctt gcggaactcg
    19501 gcgagacgcg tgccggacaa ggacggctgg ctgggaagct cgaccgtgag cgggtcgacg
    19561 tggcggccgt tcagtacgaa ctcgtagtga agatgggggc cggtcgccag gcccgaggcg
    19621 cccacccttc cgacgatggc tccctgctcc acccgcgcgc cggggcggat cccggcgccg
    19681 atcgcgctca ggtgcgcgta gcgggtgcgg atgccgcccg cgtgccggag ctccaccagc
    19741 cggccatagc ctccggcgaa acccgcccgc gtgaccacgc ccgacgcggt ggcctcgacg
    19801 ggcgctccga acggcgcccc gtagtcgatc ccttcgtgct tgcgccaggt cttcaagatg
    19861 ggatggaaac ggcggctggt gaaccggctg gtgatgcggt acgggaccgg gtagcgcagg
    19921 aaggcgccgt gcagcgagcg cccctcgtcg tcgaagtagg cccagcgggc cccccgcggg
    19981 acggggatcg cccgcagcac gcggtcctgg ttgcgcagct cgatcgccag gaagtgccgg
    20041 cttcgcacgg agccgtcggg acgcaccttg cgctccagcg ccacgcggaa gacatcgccc
    20101 ttccggaggt cgcgcgtgaa gtcgacctcc cacgcgaaga tgtcggccag atcgtagacc
    20161 agttcctcga actctccctc gcccatccgg tcgaggtcgc ctcccagctt cgccgaccag
    20221 aggctggagg tgatcacgcc ggaaatgcag accgtgtcca cgacgaaggg gaccagatcg
    20281 agctgcgacg accagctcgc gcccgagcgc gtgaactcga ggaccgagtc ggggctcagc
    20341 gtgatccgga cctggtccgg cgtgcgctgg ggaccgaagg gcccgacggg gtcgaaggcg
    20401 acgccgaccg tcatcccggg gcgcagggag cgcgggctct tgtagttctt gaggagacgg
    20461 atgatcgcct tggtctgggc gtcgtccagc cccctggccg cgaggaccgt ggagagggcc
    20521 tcgccgcggc cgagcgcgtg acgggtctgg acggggaaag gcgtggcccg gagctcggcg
    20581 tcgcgcccgt cgcccggaga tccgggcgcg cgcgggaata ccaccgccag cgagaggccg
    20641 aacatggcga cggtgagcag cgcgacgatg tggaaccagc gatccgatcg cctcacccgg
    20701 gcctaatcgg ccaaaccggc acgatcctga ccgggaattt gccacagcct ctcttcgcgc
    20761 ccccggggcc gcggctccag tatgctggtc agcctcgccg gccgaccggc gagacgatcg
    20821 gaccctgtac ggaggtcgag ccatgaagaa ccgtttcgcg ggggccaccc acgccctgct
    20881 tcgcatggtg gccgggcttc tcttcatcca ccctggcggc atgaagctct tcggttggtt
    20941 cggcggcatg ccggccggcg tcacgctcac cccccgcctc gtggcggccg ggtggatcga
    21001 gatcgtatgc ggcaccctga tcctgatcgg cctgctgacc cggccggccg ccttcctggc
    21061 ctcgggcgag atggcgttcg cctacttcat cggccacttc ccccacgggt tctggccgat
    21121 ccagaatcac ggggagccgg cggtgctctt ctgtttcctc ttcctgttct tcgccgcgaa
    21181 cggcgccggg cccgcgagcc tcgacgcgct gatacgaaaa cggcgggacc gcgtcgattc
    21241 gggcccatcc cattcgtcgt acaggtgaag cccgggcggg ccggccccga agccggccgt
    21301 ggcgggccca ctgtaagatc aatgaataaa ggagattccg tcatgcgatt catggtgatt
    21361 gtgaaggccg acaagaacag cgaagcgggc atcctgcccg acaaggaagt cttcgcgaag
    21421 atgggcaagt tcaacgaaga gctggtgaac gcgggcgtca tgctcgcggg cgaggggctg
    21481 cagccaagct ctaagggcgt gcgcatccgc ttctccggcg ggaagaagac cgtgatcgac
    21541 gggcccttcg ccgaagcgaa ggagctgatc gccgggttct ggctctggca ggtgaagtcg
    21601 aaggaggagg cgatcgagtg gctcaagcgg gccccgttcg aggacaccga ggtggagatc
    21661 cgccaggtgt acgaatcgga ggacttcggc gacgccttcc cccaggagct ccgtgagcag
    21721 gaagaaaatc tgaggggccg gatctcctcg cggaagtagc cctccactca aggccgcggt
    21781 cccccggcgg cactcgcggg gaccgcgggc cagtccaggc gccgggcatc gggcgcatct
    21841 tcaccgccgt gcccagcagc atccgcaagc gtatgatcct gcgccatgca aggtcctcat
    21901 gctggcgcag gccacgatgc ccacgccggg cacgatcccc acgccggcca cgatccccac
    21961 gccgggcacg atgtccacgc cggacacgac cgccatgcgg ggcactccgc cgccatgttc
    22021 cagcggaagt tctgggggac gctgctcctc tcgatcccca ccctcgtctg ggcgccgatg
    22081 atccagcggt ggttccgcta cgaggccccc ggcggcccgg cggcctcgcg ctggatctcg
    22141 gccgcgttcg gaaccgcggt cttcctgtac gggggcatcg tcttcctcca gggggcgcgc
    22201 ggcgagctgg ccgaccgacg ccccggcatg atgacgctga tctcgctcgc gatcagcgtc
    22261 gccttcctgt tcagcctgtt cgtcaccttc ggcttccccg ggatggatct ctggtgggag
    22321 ctcgccacgc tggtcacgat catggtgctc gggcactg
//
LOCUS       DASSLZ010000007         7045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1650046, whole genome shotgun sequence.
ACCESSION   DASSLZ010000007 DASSLZ010000000
VERSION     DASSLZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650046"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2060)
                     /locus_tag="VFM00_00245"
     CDS             complement(<1..2060)
                     /locus_tag="VFM00_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496706.1"
                     /translation="MIDADLRSYLKGATGDEPPLRHGDPRWADTELLAHRLIERSAAE
                     AALKALEQEPARKKRWDLLLLTALLQQGLGERARALDALEVVADKLLAAGDRDGVLAL
                     LPRFLEPEPNPAAVRFLHFLSQNPAASEEERADWLRTAIRIRHADPELHADLAALLER
                     SGDPDAREAAREHRLRAMELSLEDGVHEGLSEALFRAVDEDLETAPVRIGKILLRYAA
                     VAPWSDSESILDLAMAPLEERAKGRFAWDDIAPILPRLPGGPAGRALFAKLFRIVVGR
                     EPDPEAIVQGSGILDPKESFDAIAARVPKILALPPGAFVTHQTWGVGRVLSSDGDSLS
                     LDFTGRAGHKMSFAMASRSLDRLPNDGLRVLAIQHPERARALASEGDPEVIARVLRDM
                     GGTATQAQMKPRLEAALPGFDAALFWKKVKERWKADPRLDTSEAYRGQFRLAPEGSEA
                     AAATLPTIAPKAAGQGLQLIRKFLREHPEDEPRLSAHAGPLVARWSQDERLEGTMRAQ
                     ALCYALTWHSLPRETAQVILADLIASGLRPDDLALGLNQEQLLELSRGVAGEEEFLWF
                     AVESRLPRLREEGRERLRELLGPERYARAIEQRMQRASEHPALSARLVEHFAANPNDA
                     GAPTMDALLIATIRLLEGDLPEGVPERLYALVADGGLLHRRFRATPPETETAEALERT
                     VLHW"
     gene            2192..3073
                     /locus_tag="VFM00_00250"
     CDS             2192..3073
                     /locus_tag="VFM00_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7496707.1"
                     /translation="MNQLLSSGTHLIAKGALLLLSPLAVALLRFVSASVVLFAYLRFR
                     TGLARVERRDWPAFFLLGFLVVPVNQGFFLFGLARSTASHASLLYALTPLVVLLFARR
                     LLREEGLVSKLVGILAAFTGVAVILLERGLRHEVGLLSGDLLILVAVFAWAAYTVLSK
                     RLLARYDPMTVTGWSIISGTLMCLPALFVPGAIPRLESIPIGVWGAIAYLAVGTSVIA
                     YPLWSYALRHMDASKVAITTNTQPILTAALSWLLFRERFTPGFFVGAALILAGVTWVE
                     TRRPGMARAEVVPVEAG"
     gene            3150..4535
                     /locus_tag="VFM00_00255"
     CDS             3150..4535
                     /locus_tag="VFM00_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET7496708.1"
                     /translation="MRDRAGATTMTRRLPLRGDELPEVLVPPPGPKSRAAARRLRRAE
                     GAAIWGAVASPIVWSRARGSVVEDLDGNRYIDVTSGFGAATLGHAAPEVARAVSAQAR
                     RLAQGLGDLHPHVAREKLVRKLSSLGGGLSRVLVTGTGSEAVELALKTAALATGRRRI
                     VAFEGGYHGQSGAALEVTRFPPSPELLKPAGPARAIPIPFPDPRRCSARTPCEACDLS
                     CLERGWERVVAPELRGSDPPGAVIVEPIQGRAGVIVPPPAFLPRLSALARDAGMLVIW
                     DEILTGGGRTGPFWAWERSGLAAEPDLMTAGKGIGGGVAIGALFGKPKIMEVWSRHVS
                     ASGESPYASTFYAHPLACAGTLAALERLTSAEIASSREAIERTLLDVPPPPGFRIRAI
                     GAMAAIEPEDKNRSLFLPLLRHGVIAIPGGISSAVVSLYPAFTITPHQLAHAIDVMTQ
                     CAVQDEARHSG"
     gene            complement(4618..5556)
                     /locus_tag="VFM00_00260"
     CDS             complement(4618..5556)
                     /locus_tag="VFM00_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496709.1"
                     /translation="MNSYSLTHLSDAVLLRNLRIILARNGQLTAALLAHLGEVDARRL
                     YAPAGFSSMFAYCLRELRFAEGPAWRRIQAARAARRFPVLFEAVAEGRIHLTAVCLIA
                     PHLTPGNIKELLRAVGGRSRAEVQEWLASRFPQAASVPPLASIRAVALPLPKTVTLDR
                     RGDMFESPAPPSFLPPLAAVKSETTVAVIRHDLDNATVESAAVETSTTNVTCAAEVES
                     AAVPACASPADPAPAPDAGENETTESLEATTSPPRPPSFLVQVTVSKGTHDKLRLAQA
                     LLSHAVSPRDVAQVLDRALDALIEKLEKRKFGKLSH"
     gene            complement(5769..>7045)
                     /locus_tag="VFM00_00265"
     CDS             complement(5769..>7045)
                     /locus_tag="VFM00_00265"
                     /inference="COORDINATES: protein motif:HMM:NF017568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IG family 5'-nucleotidase"
                     /protein_id="HET7496710.1"
                     /translation="LVANKGYPTEILDLKYDPNFVIRGLVVDRKRGNLLKMDYFNLVT
                     RGSHGLMPLRSEERRRAYRTSRIRLGHENYVSVDTLFHLPEVYLYLCLIDLLERRRPG
                     TTLDYEALYRDVREMIDEAHRDGSLKNVITNDLDRYIRLDPELRTVLEEFRRTGKKLF
                     LVTNSEWSYADTLLRHILMKGRGAPEHWTDLFHAVVVESRKPAFFTETEAAEPIPEVK
                     GSRIACAVVRRGDAMWLERTLGASAEHVLYFGDHTYGDILQSKRARGWRTAMLVPELD
                     REITVTAEHAKEFERLARLSAERHQSEIEKAVLGRELRRLTLVLSENGNDPSRRHELG
                     GRIAEIPEQVAALERHSVELQSEIDAIRLKIDRAYNSRWGSLFREGNESSRFGHQLKD
                     FACLYMSRVSNFLHYPSDYYYQSPVGYMPHDI"
BASE COUNT         1034 a         2355 c         2470 g         1186 t
ORIGIN      
        1 ttccagtgga gcacggtgcg ctccagcgct tccgcggtct ccgtctcggg aggggtggcg
       61 cggaagcggc gatggaggag tccgccgtcg gccacgagcg cgtacaggcg ctcgggcacc
      121 ccttcgggaa gatcgccttc gaggaggcgg atggtggcga tgaggagcgc gtccatggtc
      181 ggcgctccgg cgtcgttggg attcgccgcg aagtgctcga cgagccgggc ggacagggcc
      241 ggatgctccg acgcgcgctg catgcgctgc tcgatcgcgc gcgcatagcg ctcggggccg
      301 agcagctcac gcagccgctc gcggccctct tcgcggagac ggggaagccg gctctccacg
      361 gcgaaccaga gaaactcttc ctctccggcg acgccgcgcg aaagctcgag gagctgttcc
      421 tggttcagcc cgagcgccag gtcgtcgggc cgcaggcccg acgcgatcag gtcggcgaga
      481 atgacctgcg ccgtctccct cggcaacgag tgccaggtca gcgcgtagca gagcgcctgc
      541 gcgcgcatcg tcccctccag ccgctcgtcc tgcgaccagc gcgcgacgag cgggcccgcg
      601 tgcgcgctca ggcgcggctc gtcctcgggg tgttcgcgga ggaacttgcg gatcagctgg
      661 agtccctgcc cggccgcttt gggcgcgatc gtcggcaagg tcgccgccgc cgcctcgctc
      721 ccctcggggg ccagccggaa ctgcccgcga tacgcttcgc tcgtgtccag gcgcgggtcc
      781 gctttccagc gctccttcac cttcttccag aagagggccg cgtcgaatcc cggcagcgcc
      841 gcttccaggc gcggcttcat ctgcgcctgg gtcgcggtac cgcccatgtc gcgcaggacc
      901 cgcgcgatca cttcagggtc tccctcggaa gcgagggcgc gcgcgcgctc ggggtgctgg
      961 atcgcgagga cgcgcaggcc gtcgttcggg agccggtcca gggaacggga cgccatcgcg
     1021 aacgacatct tgtgcccggc gcggccggtg aagtcgaggc tgagcgaatc gccgtcgctg
     1081 gagaggaccc gtcccacgcc ccaggtctgg tgcgtgacga aggcgccggg cggcagcgcg
     1141 aggatcttcg gcacccgcgc cgcgatcgcg tcgaacgatt ccttcggatc gaggatcccc
     1201 gagccctgga cgatcgcttc gggatcgggc tcgcggccga cgacgatccg gaagagcttg
     1261 gcgaagagcg cgcgccccgc gggtccgccc ggcaggcggg gaaggatcgg cgcgatgtca
     1321 tcccacgcga agcgtccctt ggcgcgctct tcgagcggcg ccatcgcgag gtcgaggatc
     1381 gactccgaat cgctccaggg agccacggcc gcgtagcgca ggaggatctt cccgatgcgg
     1441 accggggccg tctcgagatc ctcgtccacc gcgcggaaaa gcgcctcgct cagcccttcg
     1501 tggactccat cctccagcga gagctccatc gcccggagcc ggtgctcgcg cgcggcttcg
     1561 cgcgcgtcgg gatcgccgga acgctccagc aacgcggcga gatcggcgtg cagctcggga
     1621 tcggcgtgtc ggatccggat cgcggtacga agccagtcgg cgcgttcttc ttcggacgcg
     1681 gcgggattct gggagaggaa gtggaggaac cgcaccgcgg ccgggttcgg ctcgggctcg
     1741 agaaagcggg gaaggagggc cagcaccccg tcccggtcgc cggcggcgag cagcttgtcc
     1801 gccacgacct cgagggcgtc gagcgcgcgc gcccgctcgc ccagtccctg ctggaggagc
     1861 gcggtcagga gcagaagatc ccagcgcttc tttcgcgccg gctcctgctc gagcgctttg
     1921 agcgcggcct cggcggcgct tcgttcgatc aggcggtgcg ccagaagctc cgtgtccgcc
     1981 cagcgcgggt cgccgtggcg aagcggcggc tcatcgccgg tggcgccctt cagatagctt
     2041 cgtaaatccg catcgatcat cgttgggccc tcgcgagtcc gctaggatag cgcaaggggg
     2101 cggtgctcgg atagactctc cggcttcacg cgcgattgca cgaagggggg accggatccc
     2161 gaagcggaca cgactcatcc tggccctggg ggtgaaccag cttctctcga gcggcaccca
     2221 tctgatcgcg aagggcgcgc tgctcctgct gagccccctc gcggtggcgc tgctccgctt
     2281 cgtgtcggct tccgtcgtgc tcttcgccta tctccgcttc cgcaccgggc tcgcccgcgt
     2341 ggagcgccgc gactggccgg cgttctttct gctcggcttc ctcgtcgttc cggtgaacca
     2401 ggggttcttc ctgttcggat tggcgcgctc gacggcgagc cacgcgtccc tgctctacgc
     2461 gctgacgccc ctggtcgtgt tgctgttcgc ccggcgcctg ctgcgcgaag aggggctcgt
     2521 ctccaagctc gtcggcattc tcgccgcgtt caccggcgtc gccgtgatcc ttctcgagcg
     2581 cgggctgcgg catgaagtgg ggctgttgag cggcgacctc ctcatcctgg tcgcggtgtt
     2641 cgcctgggcg gcctacaccg ttctctcgaa gcggctgctc gcgcgctacg atccgatgac
     2701 cgtgaccgga tggtcgatca tctcggggac gctgatgtgc ctgccggcgc tgttcgtgcc
     2761 cggggccatc ccgcgcctgg aatcgattcc gatcggcgtg tggggcgcca tcgcctacct
     2821 cgccgtcggt acgtcggtca tcgcctaccc gctctggtcc tacgcgctgc gtcacatgga
     2881 cgcctcgaag gtcgcgatca cgaccaacac gcagccgatc ctgacggcgg cgctttcctg
     2941 gctgttgttc cgggagcgct tcacgcctgg gttctttgtg ggcgcggcgc tcatcctggc
     3001 gggcgtcacg tgggtggaga cgcgacggcc gggcatggcg cgagcggagg tggtgccggt
     3061 ggaggcggga tgatgttggc gaagccatgt ccgatgcgcg cggccgtgcg gcggtgccag
     3121 acgcccatgg caggacaggc ggggtcgagg tgcgtgaccg cgcgggggcg acgaccatga
     3181 cacggcggct gccgctccgg ggcgacgagc tgccggaggt actcgtgccg cctccggggc
     3241 cgaagtcgcg cgccgcggcg cgaaggttgc gccgcgccga gggcgccgcg atctggggcg
     3301 ctgtcgcttc gcccatcgtc tggtcccgcg cgcggggctc ggtcgtcgag gatctggacg
     3361 gcaatcgcta catcgacgtg acgagcggct tcggcgcggc gacgctgggc catgccgcgc
     3421 cggaagtcgc gcgtgcggtg tcggcgcagg cgcgtcgctt ggcgcagggg ctgggcgatc
     3481 tccatccgca tgtcgcgcgc gagaagctcg tgcgcaagct ctcgtcactg ggcggcgggt
     3541 tgagccgcgt gctcgtgacc ggcaccgggt ccgaggccgt ggagctggcg cttaagacgg
     3601 cggccctcgc caccgggcgc cgccgcatcg tcgccttcga gggcggctac cacgggcagt
     3661 cgggcgccgc tctggaggta acgcgttttc cgccgtcgcc ggagctgctg aagcccgccg
     3721 gtccagcccg cgcgattccc attccgtttc ccgatccgcg gcgttgttcc gcgcgcacgc
     3781 cgtgcgaggc gtgtgatctt tcgtgcctgg agcgcggatg ggagcgggtc gttgcgcccg
     3841 agcttcgagg cagcgatccg cccggcgcgg taatcgtcga gccgatccag ggacgggcgg
     3901 gcgtgatcgt cccgccgccg gcgtttcttc cgcggctgtc cgcgctggcc cgcgacgcag
     3961 gaatgctcgt gatctgggac gagatcctga ccggcggtgg ccgaacgggt cccttctggg
     4021 cctgggagcg aagcggcctc gccgccgaac ccgatctcat gacggcgggg aaggggatcg
     4081 gcggcggcgt cgcgatcggc gccctgttcg gcaagcccaa gatcatggaa gtctggtcgc
     4141 gccacgtctc cgcgtcgggc gagtccccct atgccagcac gttctacgcg caccccctgg
     4201 cgtgcgcggg gacgctggcg gcgctcgaac gattgacgtc ggccgagatc gcctcgtccc
     4261 gagaagcaat cgagcgcacg cttctggatg tgccgccgcc ccccggcttt cggatccgcg
     4321 ccatcggcgc catggccgcc atcgagccgg aagacaagaa ccggagcctg ttcctgcctc
     4381 tgctgcgcca cggagtcatc gccatccccg gaggaatcag ctccgcggtg gtcagcctct
     4441 accccgcctt caccatcacc ccccatcagc tcgcgcacgc catcgacgtg atgacccaat
     4501 gcgccgtcca agatgaggca cgccactcgg ggtaacgcga ctcaaggtga cgcgccgggc
     4561 tacgctgagc ccacgtctgg cccgttctca ccgtagcgtc cgcttccgtc gtgggcttca
     4621 gtgcgaaagt ttcccgaact tccgcttctc gagcttctcg atgagcgcgt cgagggcccg
     4681 atcgagcacc tgcgccacgt cgcgaggcga gacggcatgg ctgagcagcg cctgcgccag
     4741 ccgcagcttg tcgtgcgtgc ctttggaaac cgtgacctga acaaggaagg aaggtggtcg
     4801 agggggtgat gtcgtcgctt caaggctctc cgtcgtctcg ttctctccgg catccggagc
     4861 cggagctgga tcagcagggg acgcgcaagc tgggactgca gcggactcga cctcagcagc
     4921 acaggtcaca ttcgtggtgg acgtttcgac agcagcggac tcgactgtag cattgtccaa
     4981 gtcgtgtctg atcacggcga cagttgtctc cgatttcacc gccgctagcg gcggtaaaaa
     5041 gctgggcgga gccggggatt cgaacatgtc ccctcgtcga tcgagcgtga ccgtctttgg
     5101 aaggggaagg gcgacggctc gaatggatgc gagtggtgga acgctcgcgg cctgagggaa
     5161 acgggacgcg agccactctt gaacctcggc cctgctccgg cccccgacgg cacgaagcag
     5221 ctccttgatg ttcccgggcg tgagatgggg agcgatcaga caaacggccg tgagatgaat
     5281 gcgaccctcc gctacggcct cgaacagaac cggaaatcgc cgcgcagccc gtgcggcttg
     5341 aatgcgtctc caggcgggac cctccgcaaa gcgcagctca cggaggcagt aggcgaacat
     5401 ggacgagaac ccggccggag cgtagagcct gcgcgcgtct acctccccaa ggtgcgccag
     5461 gagcgccgcg gtcagctgac cgttgcgcgc gagaatgatg cgcaggttcc gaagcagcac
     5521 ggcatcgctg aggtgcgtca acgaatagga attcatgcaa acacctgttt gcggggaaga
     5581 gccgggttct tgggctggcg ccattggggg agtttcgttt acgccagccg ttatcggagg
     5641 ttggtccatg aaataacaag tttcggtcca gacaggcgtc caccggggac acgcggtcgt
     5701 atctgggagg gggagcgctg gacgaggcgc tcagatgcgc ccgacggccg caacagaagc
     5761 cttcaaaact aaatatcgtg cggcatgtat cccacggggc tctggtagta gtaatccgac
     5821 gggtagtgca ggaagttgga cacgcggctc atgtacagac acgcgaaatc cttgagctgg
     5881 tggccgaagc ggctcgactc gttgccctcg cgaaacagcg atccccaacg cgaattatag
     5941 gcgcggtcga tcttcaatcg gatggcgtcg atctcgcttt gcagctcgac gctgtggcgc
     6001 tccagggccg cgacctgctc cggaatctcg gcgatccggc cgcccagctc gtggcggcgg
     6061 ctgggatcgt ttccgttctc gctcagcacc agggtcagcc gccggagctc ccggcccagc
     6121 acggccttct cgatctcgct ctggtgcctc tcggccgata accgcgcgag ccgctcgaac
     6181 tccttcgcgt gctcggcggt cacggtaatc tcgcgatcca attcggggac gagcatcgcg
     6241 gtgcgccagc cgcgcgcgcg cttcgactgt aagatatccc cgtaggtgtg atcgccgaag
     6301 tagagcacgt gctcggctga ggcgccgagc gtgcgctcga gccacatcgc gtccccgcgc
     6361 cggacgacgg cgcacgcgat ccgggaaccc ttgacctcgg ggatcggctc ggccgcctcc
     6421 gtctccgtga agaacgcggg ctttcgcgac tcgacgacga cggcatggaa caggtcggtc
     6481 cagtgctccg gagccccgcg tcccttcatc aggatgtggc gcagcagcgt gtcggcgtag
     6541 ctccactcgc tgttcgtgac caggaagagc ttcttgcccg tgcggcggaa ctcctccagc
     6601 acggtgcgca gctcggggtc gaggcggatg taacgatcga ggtcgttcgt gatcacgttt
     6661 ttcaagctgc cgtcgcggtg cgcctcgtcg atcatctcgc gcacgtcgcg atagagagcc
     6721 tcgtagtcga gcgtggttcc cgggcgccgc cgctccagca ggtcgatcag gcacaggtac
     6781 aggtacacct cgggcaggtg aaagagcgtg tccaccgaga cgtagttctc gtgccccagc
     6841 cggatgcgcg acgtgcggta ggcccggcgg cgttcctcgg agcgtagcgg catcaggccg
     6901 tgggagccgc gggtgaccag gttgaaatag tccatcttca gcagattgcc gcgcttccgg
     6961 tccaccacga ggccccggat cacgaagttc gggtcgtact tgagatcgag gatctccgtc
     7021 ggatagccct tgttcgcgac cagct
//
LOCUS       DASSLZ010000008         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_763424, whole genome shotgun sequence.
ACCESSION   DASSLZ010000008 DASSLZ010000000
VERSION     DASSLZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763424"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1335)
                     /locus_tag="VFM00_00270"
     CDS             complement(112..1335)
                     /locus_tag="VFM00_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7496711.1"
                     /translation="MGYVWIIASRYLRSKRRLNFITLVSLLAIGGVFVGTAALTIVLS
                     VMNGFEDQVQRRIAGTNAHIAVLSADDRPMPENPDLVRAIRKALPTAQVAPFVYGKVM
                     VASRHGIDGMVLKGVDPPAEARVTDILAHLTPKHDAFVGGDLPGIALGRELASRLRVT
                     TGDVILISLPTEEPGGVLGGVPRTMRLRVTSIFESGLYEYDSSFGVVLAPTAASFFRL
                     DGEITGYQLRIPDMFRAAEDSRSLEQALGASYRVTNWIDLNRNLFAWMRIEKAVMFTI
                     LILIVIVAAVNIVSSLVMLVLEKRRDIGVLRTMGVTPRGILQIFLLQGTLVGLCGTAL
                     GLGAGVAVSYALGRWKLLRLPGEIYFIDTLPVKMEWNDFVFVAVAATVTCFLASLYPA
                     WQAARLAPVQTIRYE"
     gene            complement(1369..>2755)
                     /gene="lysS"
                     /locus_tag="VFM00_00275"
     CDS             complement(1369..>2755)
                     /gene="lysS"
                     /locus_tag="VFM00_00275"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET7496712.1"
                     /translation="HRSAAIRADHAALEASGQPVRVAGRLMTKRGHGKASFAHVKDED
                     GLLQIYLREDALGPEDYAQALEFDLGDWIGVEGSVFKTKTGEITVKAAAVTLLAKSLR
                     SLPEKWHGLTDVEIRYRQRYTDLVVNDEVRAVFKARARLIRSIREFLDARGYLEVETP
                     VLQPLYGGAAARPFTTHHNALDMPLYLRIADELYLKRLIVGGFERVYEIAKDFRNEGI
                     DRSHNPEFTMLEFYQAYADYETMMEVTQDLIVHAVRSLHGGTLVPHEGRTIDFAPPWN
                     RLRYKEAAAEALGVATLPEGEADLRALAERAGLKLDPALGWGRILDEVISEKVQPHLE
                     RPTFLIDYPKDVSPLAKARRGDATVVERFELIAAGMEVANSFSEQNDPLEQRRAFEAQ
                     MRQRERGDEEAQMLDEDYLRALELGMPPTGGVGIGIDRLVMLLADQRSIRDVILFPQM
                     RPEERTDEEGA"
BASE COUNT          442 a          963 c          926 g          424 t
ORIGIN      
        1 ggcatcacct ggagcgagac gtcgcgcaag acctcgaccg gcgcgacccc ttcgtcgtaa
       61 gtcttggcca ggccttccgc ttcgagcagg ggtgcaaggc cggcacccgg ctcattcata
      121 ccgaatcgtc tggaccgggg caagccgcgc ggcctgccac gcgggataga ggctcgcgag
      181 gaagcaggtc accgtcgccg ccacggccac gaagacgaag tcgttccatt ccattttcac
      241 ggggagggtg tcgatgaagt agatctcccc gggcagccgg agcagcttcc agcggcccag
      301 cgcgtaggaa accgcgaccc ccgcgcccag cccgagcgcc gtcccgcaca atcccaccag
      361 ggttccctga agcaggaaga tctgaaggat gccgcgcggc gtcaccccca tggtgcgtag
      421 aaccccgatg tcgcggcgtt tctcaagcac cagcatgacc aggctcgaga cgatgttcac
      481 ggccgccacg atcacgatca ggatgaggat cgtgaacatc accgccttct cgatgcgcat
      541 ccaggcgaac agattccggt tcaagtcgat ccagttggtc acgcggtagg aggcgccgag
      601 cgcctgctcc agggaccgcg agtcctcggc cgcgcggaac atgtcgggaa tgcgaagctg
      661 gtagccggtg atctcgccgt ccaggcggaa gaacgacgcg gccgtgggcg cgaggaccac
      721 gccgaaggag gagtcgtact cgtagaggcc cgactcgaag atcgaggtca cgcggagccg
      781 catcgtgcgc ggcacgccgc ccaggacccc tcccggctcc tcggtgggca ggctgatcag
      841 gatcacgtca ccggtcgtca cccgaagccg cgaggccagc tcgcgcccca gggcgatccc
      901 cgggagatcg ccccccacga acgcgtcgtg cttcggcgtc aggtgggcga ggatatcggt
      961 cacgcgcgcc tcggcgggcg gatccactcc cttcagcacc atgccgtcga tcccgtgccg
     1021 cgaggccacc atcaccttgc cgtagacgaa gggggcgacc tgggccgtgg gcagcgcctt
     1081 ccggatggcg cggacgagat cggggttctc gggcatcggc cggtcgtcgg ccgagaggac
     1141 cgcgatgtgg gcgttcgtgc ccgcgatccg ccgctgcacc tggtcctcga acccgttcat
     1201 gaccgagagg acgatcgtca gggccgccgt cccgacgaag acgccgccga tcgccagcag
     1261 gctgacgagg gtgatgaagt tgagccgccg cttcgagcgc aggtagcgcg aggcgatgat
     1321 ccagacgtag cccatccgtg ggtgccgggc gccgctccgg gcggagcgct aggcgccctc
     1381 ctcgtcggtg cgttcctcgg ggcgcatctg cgggaagagg atcacgtcgc ggatgctccg
     1441 ctggtcggcg agcagcatga ccagccggtc gatcccgatc cccacgcccc cggtcggcgg
     1501 catgccgagc tccagggcgc gcaggtagtc ctcgtccagc atctgcgcct cctcgtcccc
     1561 ccgctcgcgc tggcgcatct gcgcctcgaa cgcgcggcgc tgctccagcg gatcgttctg
     1621 ctcggagaag ctgttcgcga cctccatccc cgcggcgatc agctcgaagc gctccacgac
     1681 cgtggcgtcg ccgcggcgcg ccttggcgag cggcgagacg tccttgggat agtcgatcag
     1741 gaaggtcggc cgctccaagt ggggctgcac cttctccgag atcacctcat cgaggatccg
     1801 cccccatccc agcgcgggat ccagcttcag gccggcgcgc tcggccagcg cgcgaagatc
     1861 cgcctcccct tccgggaggg tggcgacgcc gagcgcttcc gccgcggcct ccttgtagcg
     1921 gagccggttc caggggggcg cgaaatcgat cgtcctcccc tcgtgcggca ccagggtccc
     1981 gccatggagg gaccgcaccg cgtgcacgat cagatcctgg gtcacttcca tcatcgtctc
     2041 gtagtccgcg tacgcctggt agaactcgag catggtgaac tcggggttgt ggctccggtc
     2101 gatcccctcg ttgcggaagt ccttcgcgat ctcgtagacc cgctcgaagc cgccgacgat
     2161 gagccgcttc aagtagagct cgtcggcgat ccgcagatac agcggcatgt cgagcgcgtt
     2221 gtggtgcgtc gtgaagggac gcgccgccgc gccgccgtag agcggctgca gcacgggcgt
     2281 ctccacctcc agataccccc tggcgtcgag aaactcccgg atcgagcgga tcaggcgggc
     2341 cctcgccttg aacacggcgc ggacctcgtc gttcacgacg agatcggtgt agcgctgccg
     2401 gtagcggatc tcgacgtcgg tcagaccgtg ccacttctcg ggaagggagc gcagcgactt
     2461 ggcgaggagc gtcaccgccg cggccttgac cgtgatctcg ccggtcttgg tcttgaagac
     2521 ggacccctcg acgccgatcc agtcccccag atcgaactcc agcgcctggg cgtagtcttc
     2581 gggaccgagc gcgtcctcgc gcaggtagat ctggagcagc ccgtcctcgt ccttcacgtg
     2641 ggcgaacgat gccttgccgt ggccgcgctt cgtcatcaac cgcccggcga cgcgcaccgg
     2701 ttgccccgaa gcctccaggg cggcgtgatc cgcgcggatc gcggcggagc ggtgc
//
LOCUS       DASSLZ010000009         8723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_257759, whole genome shotgun sequence.
ACCESSION   DASSLZ010000009 DASSLZ010000000
VERSION     DASSLZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257759"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFM00_00280"
     CDS             <1..250
                     /locus_tag="VFM00_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET7496713.1"
                     /translation="DGAVNIIYKRELDSAKDAAKKRAELVDEYNEKFANPYVAAGLGY
                     LDDVIEPQETRPRLIRALRMLAHKRQSLPPKKHGNIPL"
     gene            264..1862
                     /locus_tag="VFM00_00285"
     CDS             264..1862
                     /locus_tag="VFM00_00285"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET7496714.1"
                     /translation="MTAPRAEKAARSGAAPRSDNAPRSPGRTRAIHKVLVANRGEIAI
                     RVCRTLREMGIASVAVFSEADREAAHVYAADEALLLGPAAASQSYLNVEAVLGAAKRA
                     GADAIHPGYGFLSESAAFAEAVEQAGLVWIGPSPEAVRAMGDKVRARALAIRAGVPVL
                     PGTDGPVADPAELRRAAEQIGYPVVLKAAAGGGGKGMRRVNRPDEFEHAVKLTQGEAK
                     NAFGDDRLYLERWLEKPRHIEVQVFGDAHGSVIALGERDCSIQRRHQKVVEESPSPAL
                     SPEKRQELWDLGVRAARAGGYTNAGTAEFLMDARGNFYFLEMNARLQVEHPVTEMVLG
                     MDLVREQVRIARGERLTLTQEEVKPRGAAIEFRIYAEDPSAGFLPQAGTVRRLELPSG
                     PGVRVDFGVREGGLVPFHYDPLVGKIIVGGATREEAVARARRVLGDCRIEGIPTTLPF
                     HLWLLEQAAFVDGSYDTAYLAHHFHGLEPPPAGPEEDDAMVLAALFAHIEAVTPRVPY
                     YTPVESRWRSGAALRAEPRDGGRR"
     gene            1859..2362
                     /locus_tag="VFM00_00290"
     CDS             1859..2362
                     /locus_tag="VFM00_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET7496715.1"
                     /translation="MKVIVERDGRERLIVIEPSGAGYAALFDGHRLHVEGRVGAEMRV
                     LLDGRPVEATVRREGPDVRVAFHGREYAFRTRDARAPKLGRRGAGADRTRGELHAPMP
                     GLVVEVLAQVGDQVDAGRPLVVVEAMKMQNAFVAPLTGRISRIEVKPGMPVESGQLLL
                     AVTPGAD"
     gene            2364..3917
                     /locus_tag="VFM00_00295"
     CDS             2364..3917
                     /locus_tag="VFM00_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET7496716.1"
                     /translation="MARPPSDPAKGAKAAAGAPHALGLPGEPPFLRGVHPLMYRSRLW
                     TMRQYAGFGDAEASNARYRYLLAHGQTGLSVAFDLPTQMGYDSDHPRASGEVGRTGVA
                     IATLEDMERLLDQIPLDQVSLSMTINATAMILLALVVAVADRRGIARGKLSGTVQNDV
                     LKEYIARGTYIYPPAASLKIATDLFEFGADEVPQWNTISVSGYHIREAGSTAVQEVAY
                     TLANGLAYVGAAVDRGLPVDRFAPRVSFFFNAHNDFLEEIAKFRAARRLWSRLMEERF
                     HPSDTNSLKLRFHAQTAGSTLTAQQPHNNIARVALQGLAAVLGGCQSLHTNSFDEAIS
                     LPSEEAATVALRTQQLLAYESGVAATVDPVGGATAIEAMTDQIEREARAEIEAIDAMG
                     GTVAGIERGYQARKIEESAYQTQKEIDKDERIIVGVNRFGDRDDMGRPSFLLDPGLET
                     AQAARVRAFKASRDAARAAQAAGRVEETARRDGNLMPAVIDAVQAGVTLGEISDALRR
                     VYRTYDPSR"
     gene            3975..4805
                     /locus_tag="VFM00_00300"
     CDS             3975..4805
                     /locus_tag="VFM00_00300"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET7496717.1"
                     /translation="MTPIQAAILGIVEGLTEFLPVSSTGHLILAGHAMGLSGSAVDAF
                     EIVIQAGALLAVVWLYRARIGEILAGIFRNNAGGRALLIRLIVAFLPAAVIGFLTHSW
                     IKEHLFGPRPVAIALLLGGIAILVVERMRHHARQPIAAVVTEIPVWGALVIGLAQVLS
                     LWPGTSRAMTTILAALLVGATPVVAAEFSFLLALPTLGAATLFDLVKHHDELLGPAMG
                     GPAPLVIGLATAAIVAAIAIRGFLTYLTRRGLAPFGWYRIVIGIVLIAVLWTHPGALG
                     "
     gene            4840..5247
                     /gene="mce"
                     /locus_tag="VFM00_00305"
     CDS             4840..5247
                     /gene="mce"
                     /locus_tag="VFM00_00305"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HET7496718.1"
                     /translation="MIRGLAHVGIAVRSLEPAIERWTKGLGFTHESTETLEVLGLRLA
                     FLRAGDAVIELLEPTEPDAVVAKYLERRGEGIHHLSFFVDDLEGALRDAEAAGLSLID
                     RTPRAGSHGTQVAFLHPGSLGGVLVELCRKQGA"
     gene            5248..6762
                     /locus_tag="VFM00_00310"
     CDS             5248..6762
                     /locus_tag="VFM00_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496719.1"
                     /translation="MRAMFRLAAAGTRGLDALASRPRAALMAATLAALLTGCAGLGPG
                     TGPPSTSTLEAGPGSGYLTPHSAYLRPLREDVTVRAILTVGDTLTSTRVEDSSYVFYP
                     QPDGLAARLAGNGLVEVYMTHDLDWTVGAGSARVSRLLLNQRTGGVLNADYLLDGNEG
                     YVALAATTLVGPREGFLGPTLLINESSTDGPRHGAVAAVDVRAGTITELPQFGRFRHA
                     ATIIIPHSSGALLALETEEGAPGLSQLWMYAASNASDLVLGRGRLYVLRADRPEFGYD
                     TRYASMALRNRPISGRFVPCDDPQEFAIARQPDALEQRAQDAGCLNFVRLAGIQADPD
                     RSDAFYFTDLGAPSPADPSTGRPVTQNGRLYRAELDPIDPTRVTRLEVVLDGDESDDI
                     YRPDNIAAGRDAILIQEDPRARGLHAARILRYDTLTRRLDPIAACAERDIQGRLLPAG
                     TGGQWESTGITEAGSLFGEGAWLVAVQAPTDWSTPFRGSGGGQLLLMRAPEARR"
     gene            complement(6767..7495)
                     /locus_tag="VFM00_00315"
     CDS             complement(6767..7495)
                     /locus_tag="VFM00_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496720.1"
                     /translation="MNRGLGISGAGRFDAGAVLLLAALLGVLGAPRPTLAQEGTSPAL
                     TDSMAQSIEQSAPPSESAPPSTQPERPPLDVPPGRFVAEVLDSTYFIEPAEFFALDLP
                     VRAQGGAMAAHMLGDISVVGGRHDIIVRLFRSGDYQNWLKRRGGRQAGPIWSSPRQRV
                     VHLDQELPQGTPLVLLLDNGYSLRTPKRVRVQVQLQYQGEPGAAQNVSAQPVRAPQEG
                     DITPRSNSDEEFPPPPPPPPSEGK"
     gene            complement(7512..8618)
                     /locus_tag="VFM00_00320"
     CDS             complement(7512..8618)
                     /locus_tag="VFM00_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HET7496721.1"
                     /translation="MRSKVAVLKTTPETAVRDFGRLMELADMRQELRPETTTLLKVNI
                     SWHVYYPACSTSPWQLEGVIQGLLGAGFDPKRILAAQNSTVVVDPHVGVRNNKLEPVL
                     ERYGIRSIWLNDPESEADWIRYEPKGKMLVLNDVYPDGLMIPKSLIGANIVHLPTLKT
                     HVFTEMTGAMKNAFGGLLHLKRHWTHSVIHETLVDLLTIQKEIHPGVFAVMDGTISGD
                     GPGPRAMRLRVTNLMLASRDQVAIDAVAAKLMGFDPMTIKFIRLANDAKLGNGDPRCI
                     DLVGDDVSGVNLGYSRNENTFASKGQKLIYHGPLKPLEKFLLQSPLVPWSYFASRLYH
                     DGYWYRFVGTKRKKEVMKSGWGRLFRDYEPKGQE"
BASE COUNT         1253 a         3108 c         3046 g         1316 t
ORIGIN      
        1 ggacggagcg gtgaatatta tttacaagcg agagctggat tcggccaagg acgccgccaa
       61 gaagcgggcc gagctggtgg atgagtacaa cgagaaattc gcgaacccct acgtcgcggc
      121 cgggctcggg tatctggacg acgtcatcga gcctcaggag acacgcccgc ggctgatccg
      181 cgcgctccgc atgctcgcgc acaagcgcca gtcgcttccg ccgaagaagc acggcaacat
      241 cccgctgtag cgggagcccg ccgatgaccg cacctcgcgc cgaaaaagcc gcccgctccg
      301 gggccgcccc tcgatccgac aacgctcccc gctcgcccgg ccggacgcgc gccatccaca
      361 aagtgctcgt cgccaatcgc ggcgagatcg cgatccgcgt ctgccgcacg ctgcgggaga
      421 tgggaatcgc gtcggtcgcg gtcttctctg aggccgatcg cgaggcggcg cacgtctacg
      481 ccgcggacga agcgctgctc ctcggtcccg ccgccgcgtc gcagagctac ctgaacgtcg
      541 aggcggtcct cggcgccgcc aagcgcgccg gcgccgacgc catccatccc ggatacggat
      601 ttctctccga gtcggcggcc tttgcggaag cggtggagca ggccgggctc gtctggatcg
      661 gtccctctcc cgaagccgtc cgcgccatgg gcgacaaggt gcgcgcccgc gcgctcgcga
      721 tccgggcggg cgtcccggtg ctccccggca cggacggccc cgtcgccgat cccgccgagc
      781 ttcgccgcgc ggccgaacag atcggctacc cggtcgtgct caaggccgcg gccggcggcg
      841 gcggcaaggg aatgcgccgc gtgaaccgtc ccgacgagtt cgagcacgcg gtcaagctca
      901 cgcagggaga ggcgaagaac gccttcggcg acgaccggct ctacctcgag cggtggctgg
      961 agaagccgcg gcacatcgag gtccaggtgt tcggcgacgc gcacggctcc gtgatcgcgc
     1021 tgggcgagcg cgactgctcg atccagcggc ggcatcagaa ggtggtcgag gagtcgccgt
     1081 cccccgccct ctcacccgag aagcgccagg agctgtggga tctgggcgtg cgcgcggcgc
     1141 gcgcgggcgg ctacaccaac gccggcacgg ccgaattcct gatggacgcc cgtggcaact
     1201 tctactttct cgagatgaac gcgcggttgc aggtcgagca ccccgtcacc gagatggtgc
     1261 tggggatgga cctggtgcgc gagcaggtgc ggatcgcgcg cggcgagcgg ctcacgctga
     1321 cgcaggaaga ggtgaaaccg cgcggcgccg cgatcgagtt ccggatctac gccgaggacc
     1381 cctcggcggg cttcctgccg caggcgggca cggtgcggcg cctggagctg ccctcgggcc
     1441 cgggggtgcg cgtggatttc ggcgtgcgcg agggcggcct cgtcccgttc cactacgatc
     1501 cgctcgtcgg caagatcatc gttggaggag ccacgcgcga ggaggcggtc gcgcgcgcgc
     1561 gccgcgtcct cggcgactgc cggatcgagg ggatcccgac gacgctcccc ttccatctct
     1621 ggctgttgga gcaggccgcg ttcgtggacg ggagctacga cacggcgtat ctcgcccacc
     1681 acttccacgg tctcgagcct ccgcccgcgg gccccgagga agacgacgcc atggtgctcg
     1741 cggcgctctt cgcgcacatc gaggcggtca cgccgcgcgt gccgtactac acgccggtcg
     1801 agagccggtg gcgctcgggg gctgccctcc gggccgagcc gcgcgacgga ggccggcgat
     1861 gaaggtgatc gtcgagcgcg acggccggga gcggctgatc gtgatcgagc cgagcggagc
     1921 cgggtacgcg gcgctcttcg acgggcaccg cctgcacgtc gaggggcgag tcggagccga
     1981 gatgagggtg ctcctggacg ggcgccccgt cgaagccacg gtgcgccgcg aaggccccga
     2041 cgtgcgcgtc gccttccatg gccgcgagta cgcgttccgc acgcgcgacg cgcgcgctcc
     2101 caagctgggc cgccgcggag ccggcgcgga ccggacccgg ggcgagctgc acgcaccgat
     2161 gcccggcctg gtcgtcgagg tgctcgccca ggtgggcgac caggtggacg ccgggcgccc
     2221 tctggtcgtg gtcgaggcga tgaagatgca gaacgccttc gtcgcgccgc tcacgggccg
     2281 catcagccgt atcgaggtga agcccggcat gccggtcgag tcggggcagc tgctgctcgc
     2341 ggtgacgccc ggtgccgact gacatggcgc gacctccgtc cgacccggcc aagggcgcga
     2401 aggccgcggc gggagcgccg cacgcgctgg gcctccccgg cgagcctccg ttcctccgcg
     2461 gcgtccatcc gctcatgtat cgctcgcggc tctggacgat gcgccagtac gcgggattcg
     2521 gcgacgccga ggcctccaac gcgcgctacc gctatctgct cgcacacggc cagaccgggc
     2581 tttccgtcgc gttcgaccta cccacccaga tgggctacga ctccgaccac ccccgcgcct
     2641 ccggagaagt gggacgcacc ggcgtcgcga ttgccacgct cgaggacatg gagcggctgc
     2701 tcgaccagat cccgctcgac caggtgagcc tctccatgac gatcaacgcg accgcgatga
     2761 tcctccttgc gctcgtggtc gccgtggcgg atcgccgcgg gatcgcgcgg gggaagctct
     2821 ccggcacggt gcagaacgac gtactcaagg aatacatcgc gcgcggcacc tacatctatc
     2881 cccccgcggc gtcgctcaag atcgcgaccg atctcttcga gttcggagcc gacgaggttc
     2941 cccagtggaa cacgatctcg gtgagcggct accacatccg ggaggcgggc tcgacggcgg
     3001 tgcaggaagt ggcgtacacg ctggcgaacg ggctcgccta cgtgggcgcc gcggtggatc
     3061 gcgggcttcc ggtggatcgc ttcgcgccgc gcgtctcctt cttcttcaac gcccacaacg
     3121 attttctgga agagatcgcc aagttccgcg cggcgcgccg cctctggtcg cggctcatgg
     3181 aggaacgctt ccacccgtcg gacacgaact cgctcaagct ccgcttccac gcgcagacgg
     3241 ccggctccac gctgaccgcg cagcagccgc acaacaacat cgcgcgcgtg gcgctccagg
     3301 ggctggcggc ggtgctgggc ggctgccagt cgctccacac gaactcgttc gacgaggcga
     3361 tctcgcttcc cagcgaggag gcggccacgg tcgcgctccg gacgcagcag ctgttggcct
     3421 acgagtcggg cgtggcggcg acggtagatc cggtgggcgg cgccacggcg atcgaagcga
     3481 tgaccgacca gatcgagcgc gaggcgcgcg cggagatcga ggcgatcgac gcgatgggcg
     3541 gcaccgtggc cggcatcgag cgcggctacc aggcccggaa gatcgaggag agcgcctatc
     3601 agacccagaa ggagatcgac aaggacgagc ggatcatcgt cggcgtgaac cgcttcggcg
     3661 atcgcgacga catggggcgt ccctcgttcc tgctcgatcc cggcctggag acggcgcagg
     3721 cggcccgcgt gcgcgcgttc aaggcgagcc gcgacgccgc gcgcgcggcc caggcggccg
     3781 ggcgcgtgga agagaccgcg cgtcgggacg ggaacctcat gcccgccgtg atcgacgccg
     3841 tccaggcggg ggtcacgctg ggcgagatca gcgacgcgct ccggcgcgtc tatcggacct
     3901 atgatccctc gcgctgagca ctctcgccgc gcgtggcgcg cgacccgtgg ggtgacgatg
     3961 accggagaac gttcatgacc ccgatccagg ccgccatcct cggcatcgtc gaggggctca
     4021 ccgagttcct ccccgtctcg tccaccgggc acctgattct cgcggggcat gccatgggcc
     4081 tctccgggag cgccgtggac gccttcgaga tcgtgatcca ggcgggcgcc ttgctcgccg
     4141 tggtgtggct ctatcgcgcg cggatcggcg agatcctggc ggggatcttc cgtaacaacg
     4201 ccggcggacg cgcgctcctg atccgtctga tcgtggcgtt ccttcccgcg gccgtcatcg
     4261 gcttcctcac gcactcgtgg atcaaggagc acctcttcgg cccgcgcccg gtcgcgatcg
     4321 cgctcctgct cggcggcatc gcgatcctgg tcgtggagcg gatgcgccat cacgcgcgcc
     4381 agccgatcgc ggcggtggtc accgagatcc cggtctgggg cgcgctcgtg atcggcttgg
     4441 cccaggtgct gtcgctgtgg cccggcacgt cgcgcgccat gaccaccatc ctcgccgcgc
     4501 ttctggtggg cgccacgccg gtcgtggccg ccgagttctc gttcctgctg gcgctcccca
     4561 ccctgggcgc ggccaccctg ttcgacctgg tgaaacatca cgacgagctg ctgggccccg
     4621 ccatgggcgg accggcgccg ctcgtgatcg ggctcgcgac ggccgccatc gtggcggcga
     4681 tcgcgatccg cggctttctg acctacctca cccggcgcgg cctggcgccc ttcgggtggt
     4741 accggatcgt gatcgggatc gtcctgatcg ccgttctctg gacgcacccg ggagcgctcg
     4801 ggtgaccggg gcgctccggc gtgccgccat gaggcgccca tgatccgggg tctcgcccac
     4861 gtcggcatcg ccgtgcgctc gctggagccc gccatcgagc gctggaccaa ggggctcggc
     4921 ttcacgcacg agagcaccga gaccctcgag gtcctgggat tgcgcctcgc tttcctgcgc
     4981 gccggcgacg cggtgatcga gctgctcgag ccgaccgagc ccgatgcggt ggtcgcgaaa
     5041 tacctcgagc gccgcggcga ggggatccat cacctctcct tcttcgtgga cgacctggag
     5101 ggggcgctcc gcgacgccga ggccgcgggc ctctcgctga tcgaccgcac gccgcgcgcg
     5161 ggaagccacg gcacccaagt cgcgttcctc catccggggt cgctgggcgg cgtgctcgtg
     5221 gagctctgcc ggaagcaggg ggcctaggtg cgcgcgatgt tccggctcgc cgcagcaggc
     5281 acgcgcgggc tcgacgcgct cgcctcccgc ccgcgcgccg cgctcatggc cgcgacgctc
     5341 gccgcgctcc tcaccggctg cgccggactg ggccccggca cgggaccgcc ctcgaccagc
     5401 accctcgagg ccggaccggg ttcgggctac ctcacgccgc attccgcgta cctccgcccg
     5461 ctccgggagg acgtcaccgt tcgcgcgatc ctgaccgtcg gcgacacgct cacctccacc
     5521 cgcgtcgagg actcttcgta cgtcttctac ccccagcccg acggcctcgc ggcgcgcctg
     5581 gcggggaacg ggctggtcga ggtctacatg acgcacgacc tcgactggac ggtgggcgcg
     5641 gggtcggcgc gcgtgtcgcg cctgctgctg aaccagcgga ccggcggggt gctgaacgcc
     5701 gactatctgc tggacggaaa cgaggggtac gtcgcgctcg ccgccacgac cctcgtcggc
     5761 ccgcgcgagg gcttcctcgg tcccaccctg ctgatcaacg aatcctccac cgacggtccg
     5821 cggcacggcg ccgtcgccgc ggtcgacgtc cgcgcgggaa cgatcaccga gctgccccag
     5881 ttcggccgct tccgccacgc ggccacgatc atcatccccc actcctccgg agcgctgctc
     5941 gcgctcgaga ccgaggaggg tgcgccgggc ctgtcgcagc tctggatgta cgcggcgagc
     6001 aacgcttcgg acctggtgct ggggcgcggg cggctgtacg tgctgcgcgc cgaccggccg
     6061 gagttcggct acgacacgcg ctacgcctcg atggcgctcc ggaaccggcc gatctccggg
     6121 cgcttcgttc cctgcgacga tccccaggag ttcgcgatcg cgcggcagcc ggatgcgctg
     6181 gagcagcgcg cgcaggatgc gggctgtctc aacttcgtcc gtctcgcggg catccaggcc
     6241 gaccccgacc ggtccgacgc cttctacttc accgacctgg gcgcgccctc gccggccgat
     6301 ccctcgaccg ggcgtcccgt cacgcagaac ggccgtctgt accgcgccga gctcgacccg
     6361 atcgacccga cccgcgtcac gaggctggag gtggtgctgg acggggatga gagcgacgac
     6421 atctaccgcc ccgacaacat cgccgccggg cgcgacgcga tcctgatcca ggaggacccg
     6481 cgcgcccgag gactccacgc cgcgcgcatc ctgcgctacg acacgctgac gcggcgcctc
     6541 gatccgatcg cggcctgcgc ggagcgggac atccaggggc ggctcctgcc ggccggaacg
     6601 ggcgggcagt gggaatccac gggaatcacg gaggcggggt ccttgttcgg cgagggggcg
     6661 tggctggtgg ccgtgcaggc ccccaccgat tggagcacgc cgtttcgcgg tagcggcggc
     6721 ggtcagctgc tgttgatgcg cgcgcccgaa gcgcggcgct agaagactac ttcccttccg
     6781 acggcggcgg gggaggcggc ggcgggaact cctcgtcgct gttggagcgc ggcgtgatgt
     6841 ccccttcctg cggggcgcgc accggctgcg cggagacgtt ctgcgccgcg cccggctctc
     6901 cctggtactg cagctggacc tgcacccgca cgcgcttggg cgtgcggagc gaatagccgt
     6961 tatcgagcag gaggaccagc ggcgtcccct gcggcagctc ctgatcgagg tgcacgacgc
     7021 gctgccgggg cgaggaccag attggtcccg cctggcggcc gccgcggcgc ttcagccagt
     7081 tctggtaatc cccggaccgg aagagccgca cgatgatgtc gtgccgcccg ccgaccacgc
     7141 tgatgtcgcc cagcatgtgg gcggccatgg cgcccccctg cgcgcgaacg ggcagatcga
     7201 gggcgaagaa ctccgcgggc tcgatgaagt aggtggagtc gagcacctcg gcgacgaatc
     7261 gccccggagg cacgtcgagc gggggccgct ccggctgggt ggacggcgga gccgattccg
     7321 atgggggcgc cgactgctcg atcgactgcg ccatcgaatc ggtgagcgcg ggcgacgtgc
     7381 cctcctgggc cagcgtcgga cgaggcgcgc ccagcacgcc gaggagcgcg gccaggagca
     7441 gcaccgctcc cgcgtcgaag cgtccagcgc cggagatacc aagtcctcgg ttcaccacgt
     7501 cgcgtcctcc cctactcctg tcccttcggc tcgtagtcgc ggaagaggcg gccccagccg
     7561 ctcttcatga cctctttctt ccgcttggtg cccacgaaac ggtaccagta cccgtcgtgg
     7621 tagagcctcg aggcgaagta cgaccacggc accagcgggc tctggagaag gaacttctcc
     7681 agcggcttca gcgggccgtg gtagatcagc ttctgtccct tcgacgcgaa ggtgttctcg
     7741 ttccgcgagt agcccaggtt cacgccgctc acgtcgtcgc ccacgagatc gatgcaccgc
     7801 gggtcgccgt tgcccagctt cgcgtcgttc gcgaggcgga tgaacttgat cgtcatcgga
     7861 tcgaagccca tcagcttcgc cgccaccgcg tcgatcgcga cctggtcgcg cgaggcgagc
     7921 atcagattcg tcacccggag ccgcatcgcg cgcgggccgg gcccgtcgcc cgagatcgtg
     7981 ccgtccatga cggcgaagac gccggggtgg atctccttct ggatcgtgag caggtccacg
     8041 agcgtttcgt ggatcacgct gtgcgtccag tgccgcttca ggtgcaacag cccgccgaac
     8101 gcgttcttca tcgcccccgt catctccgtg aagacgtggg tcttgagcgt cggcaggtgg
     8161 acgatgttcg ccccgatcag gctcttcggg atcatgagcc cgtcgggata gacgtcgttc
     8221 aggaccagca tcttcccttt gggctcgtaa cgaatccaat cggcctcgga ctccggatcg
     8281 ttgagccaaa tcgagcggat cccgtatcgt tctaacaccg gttccagctt gttgttccgc
     8341 acgccgacgt ggggatccac cacgaccgtg gagttctggg cggcgagaat gcgcttgggg
     8401 tcgaaaccag cccccaaaag cccctggatc accccttcga gctgccaggg gctggtggag
     8461 caggccgggt agtagacgtg ccaggagatg ttcaccttga ggagcgtggt cgtttccggc
     8521 cgcagctcct gccgcatgtc ggccagctcc atcaggcggc cgaagtcccg cacggcggtc
     8581 tcgggggtcg ttttcaggac ggcgaccttg gatctcatgg aaatcaggca gctccctttc
     8641 cgtctttcgg gtgcagcacc gaattggcga tcacgatgcg ctggatctcc gacgtccctt
     8701 cgtacagctc ggtcaccttg gcg
//
LOCUS       DASSLZ010000010        13978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_384813, whole genome shotgun sequence.
ACCESSION   DASSLZ010000010 DASSLZ010000000
VERSION     DASSLZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384813"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="VFM00_00325"
     CDS             <1..1350
                     /locus_tag="VFM00_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7496722.1"
                     /translation="LSDEVALVTPLLLIGLDRWGSPRVAERGRRTVYWGFTAVAVAGL
                     LARLGAHSLRLNEPHDQVAAGTAFWAPLIATGEYLRALLVPYPLNAMRSLTTPDAASW
                     GLRLQALGGVALIALFVWLRRKDPLARTGALLLFLPLLPALPIGPFQGTFVEERAAYF
                     ASTGLVFLMASLVAGIAGRPRETRIAAAAVMAAVVLGAGALTMNRLPVWSSNVALLDD
                     AVRHDPRDPAAHVALADQYIAEGNYHAALASLDRAIAVDSTRALAWHRRTLLLNRMGK
                     LPEAEASGRHAVAIQPSEAIFWANLGDILTREGKTHDANAANGRAVTLDPKNPDNWYN
                     YGVSLAASDSLPQAMQAYRRAIGLNPGHFQAVNNLGAALAISGRIAEARDVYMKAVEL
                     EPSSVQARMNLALAYLRLGDVAGASAQREAVQKLDQAAAMQLVEMIKQVQVGKGATR"
     gene            1385..2179
                     /locus_tag="VFM00_00330"
     CDS             1385..2179
                     /locus_tag="VFM00_00330"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HET7496723.1"
                     /translation="MTPALDTRLFDPSRINLLVGLGIFLIFLIINISRARRGAGMFIR
                     PLAGLKAVEEAIGRATEMGRPVLYVPGLGDMSEVGTLASITILSRVARRTAEMGCDLE
                     VPGYDPVAATIMEGAVRQAYIEAGRGDAFRPGSVHYVSAQQFAYVAAVTGYMLRARPA
                     TNIFMGSFYAESLFLAETGNLTGAIQIAGTDQVTQLPFFVCACDYTLLGEELYAASAY
                     LGKEPIQLATIAAQDWAKALAAATVVVASILMALGHPEVGKLLHTQ"
     gene            2192..2809
                     /locus_tag="VFM00_00335"
     CDS             2192..2809
                     /locus_tag="VFM00_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496724.1"
                     /translation="MRFGPLLVTFVTGALLVAFFFVPHHAAQEAQSVMLDWANVVYAF
                     ALVLGSVSLWTAHQKKVTTRAPGWIYSVVTLASLAFVTIWGCVQGVEEGTAVNAFYNT
                     VNAPLASTMFALLAFFIASAAFRAFRARNAEATLLLVTAIVMMIGRVSIGEYLWPGFP
                     KVTEWLLDVPNLAAKRAVAIGVGLGAISTALKIILGIDRSYLGRS"
     gene            2829..3668
                     /locus_tag="VFM00_00340"
     CDS             2829..3668
                     /locus_tag="VFM00_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496725.1"
                     /translation="MNGPRQSIWTRLLRLDRRIIFVLIALATAIPLLRPVNLPIAVSP
                     RVRAAYDTVDKLPRGSYVLMSLDYEPDIMAELQPMAKAVLRHCFRKGLKPVVLTLYPA
                     APGLIEEALRVSATAEGKKRNVDYVFLGYKSGSQSVILGLGESIRTMFPTDFWGTPLN
                     QIPMMKGHNSFADFPLVINLSGSSIADYYVRIAATRYRRPMILGSTAVMTADYYPYLS
                     AGQILGLIGGMKGAAEYERLMDLFGDARRGMDAQSLVHVVIVVLVILGNLALFSSGGL
                     RRS"
     gene            3703..4320
                     /locus_tag="VFM00_00345"
     CDS             3703..4320
                     /locus_tag="VFM00_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496726.1"
                     /translation="MNPVTQNWIAAFLTLCVFSFLYRDNPFYRFAEHLFVGAAAGYLL
                     AVQYQNVIKPNVIGEMNQGNMLPLVPLALGIILLGRLWPRTSGIARWSLAFYVGIYSG
                     IAIPSYLQAQIFAQLADQVKPFAPGWGAVNSALILIGFITVLSYFFFSAAHRGLHGLS
                     ARIGIWFLMVAFGASFGYTVMARVSLLIARMQFLLRDWLHLLPPA"
     gene            complement(4342..6495)
                     /locus_tag="VFM00_00350"
     CDS             complement(4342..6495)
                     /locus_tag="VFM00_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7496727.1"
                     /translation="MHGESTFLRDLVVALAAALVVLLPSRRFRIPAAVGFMLTGILIG
                     PGALGWIADTAHVSALAEVGVSVLLFIVGLEFSLARLREIGRAFLVAGPLQVVATIAA
                     VTGVLLLLPIHARLAGAVFAGMLVTLSSTAMVMRSLGERGETHAPQGRLLVGILLFQD
                     IAMVPMLLLVPALAAAAGAVGAAGAVGDGGAAAVGALSGGSGALSGGSGALSGSGTLS
                     LAARLLLGVAIAGLVIAAARWLMPRIVNAVIRSGVRELYVMMAVAVCLGSALLTQRLG
                     LSPALGAFLAGLLVSESEYSHQVVSEIVPFRDLFSSLFFISIGMLLEPAFVAANLPSI
                     GGWLFLTVALKAIVAAAIVRLMGFPLRVAAIVGISLAQVGEFSFVLASVGIRNGLLSA
                     AQSREFFAVAVLSLVITPFLVPFGSWLGAREAARATGAGASASCSGVARFGGRAREEG
                     SGTGTEAGALRDHVIVAGYGLNGRNVAHALAELGIHYVVLEMDPSNVRRARAQGVPIQ
                     FGDVSRPESLHACGVERAAVVVLAISDASATRHAVSQVRRANPGAYIIARTRLVAEVA
                     ELRRLGADEAIPEEFETSIAIVTRVLRRYHVPGNVIRLQEGALRREGYAFLRGGDVSV
                     ELTHSIERMIAGAVTDTFFVEPGSPAAGHTLASLDLRGRTGALVIAIVRAGKPKISPE
                     ADLELEIGDIMVLVGDHQALESAFRILEGPAVQPG"
     gene            complement(6554..7747)
                     /locus_tag="VFM00_00355"
     CDS             complement(6554..7747)
                     /locus_tag="VFM00_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HET7496728.1"
                     /translation="MSRSDFPVDALRARFPALREAGDREIFFDNAAGAQVPDDVIEAM
                     REHLVRRGVNRGGLFARSIEVDRQIAAAREAVAAFLNARSPEEIVFGLNSTSLIRTVA
                     EAVRPLFSAGDRVIVTQLDHEANVGPWLRLERDGVVPLVWKVRGTEARLDLDDLRELL
                     RESDGRARLVAMPLASNAIGRIVDVAAAARVAHEAGALAFVDAVHFAPHGPIDVQALG
                     ADFLAFSGYKIFGPHIGFVWGRADSYRRLTPVHDFFIPAEAPYGYEGGTQNFEAMAGM
                     AAALRYVMEIGAERIRAYETELASALLEELRRVPGLSILGDADPARVEARVPTVAFTI
                     ERSVPRAIVERLAERGIHAREGHMYAPRLLEAVGRDAVARVSLCHYNTRAEITRLGEA
                     LRSLG"
     gene            complement(7734..8039)
                     /locus_tag="VFM00_00360"
     CDS             complement(7734..8039)
                     /locus_tag="VFM00_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET7496729.1"
                     /translation="MTQFVKVARASDVEEGKGLIVWAGPIKVALFRCEGAVYAIRNQC
                     PHMGGDLGEGLLTGDVVRCPWHGWKFSVKTGKSPEADLVAVRSFEVKVEGEDVYVAI"
     gene            complement(8055..10157)
                     /locus_tag="VFM00_00365"
     CDS             complement(8055..10157)
                     /locus_tag="VFM00_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET7496730.1"
                     /translation="MPLIRAALLAAVFLGVVATARASTMPAVADSSGPAPDSSGPAPD
                     SSDIPPAPDSIRYSLPGVDVRAPRFQGIDPRADFITREQIGRTAATADDAFRVVQTLP
                     GVASSDYAASFLVRGGESDETLVRFDGFDLLEPYHIPYWGGAISVVSPDVVQTMQLSR
                     GGLPARYGRELSGALEIQSPSDRPEGPRYQVGAGPTQLRALVAGPTKGGGSYLLGIRH
                     GLLAAIGRLHRLDRDASIVPDFQDLIGEARLRPAEGHEVTFLALGARERLRYDMPYDE
                     NDLNGSVRNLTLGASWSYRPSERVQHRLVFSADRFHRATLVGRSGRDDSVTRALRARL
                     EGELSLGRGKALEWGAAAEYEDGWLALDGIRGSLGTSGYQEEVEHVIAGTAIRRRGEA
                     YLSARASAGSRATVTAGVNVSRDLYAWGLRQDGAPLPGTPGFAFVSPRLGVLARLGSA
                     GSAWASMGLMRQPSLLNNLERESLPLGRNREAAETAAGFEVAPAGARFRVEGYLRKER
                     GAGLPAQDVTAQPSPPFALDRGTARGVELSLRTPSWARADASLGYTLSRALWTAPEGI
                     VPRSFDQRHAAALSLNVRPAAGWNLNVLARYHTGSPYTASVWARDDSTGTWTRGFGTF
                     MGARYPAYFRLDFRLSHPLSFGPTGGQAYVELINATGRVNVHQYTYVFDPGADAAPRL
                     QAVELFPRMPAAGFEFSF"
     gene            complement(10362..10760)
                     /locus_tag="VFM00_00370"
     CDS             complement(10362..10760)
                     /locus_tag="VFM00_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET7496731.1"
                     /translation="MITLSEKAASEIRTIMEREGGTFEGVRVFVAGGGCSGLSYGMQI
                     ADEPATADDLVFEAGGVKVIVDVQSHQFLEGASIDFDDSLQGGGFKINNPNAVKSCGC
                     GSSFATEESEGAAPGGGCGCGGSGGGCGSH"
     gene            complement(10897..12333)
                     /gene="pyk"
                     /locus_tag="VFM00_00375"
     CDS             complement(10897..12333)
                     /gene="pyk"
                     /locus_tag="VFM00_00375"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET7496732.1"
                     /translation="MRRTKIIATVGPASADPAVLARMARAGMDVARINFSHGTYTDHR
                     RAMRAVRLAAHRARRTVGILLDLQGPRLRVGSFRDGSIRIAAGAEVLLTSEGGIGTAE
                     RIPIDYSGLMQDVSVGDRVLIDDGKVELKAVRKAAGGLRCRVVTGGAISDHKGVNFPG
                     VRLSAPALTAKDRRDLAFGIEEGIDFVALSFVRSAEDLIGLRRLLRRAQRPPMVIAKI
                     ERREAIENLESILEAADGVMVARGDLGVEYPPERVPILQKRIIERANALEVIVITATQ
                     MLESMVTSSSPTRAEASDVANAIFDGTDAVMLSAETAVGRYPERAVRTMARIAGEADE
                     FAATKRTPRRSDGGALPSPTHALAHTAYQASREIRAKALAIFTHTGYSARLVSKSRPQ
                     APIFALTPLESTCRRLSLAWGVSAVQVPRWRTAERMVEAGLRILITRKMIRRGDWVVA
                     MAGTTTRSGGTNMLRILQQGRPPDKPRR"
     gene            complement(12347..12802)
                     /gene="bcp"
                     /locus_tag="VFM00_00380"
     CDS             complement(12347..12802)
                     /gene="bcp"
                     /locus_tag="VFM00_00380"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HET7496733.1"
                     /translation="MTVGMPAPDFELPSTLGRKVTLHEFRGKRVILYFYPKDDTPGCT
                     MEACAFRDNLPRFQAKDGIVLGISLDDELSHQRFAQKYNLPFPLLSDVDAAVCRQYGV
                     YKEKNMYGRSFWGIERTTFVVDREGKVENVFRRVKVEGHADEVLATLAV"
     gene            complement(13475..>13978)
                     /locus_tag="VFM00_00385"
     CDS             complement(13475..>13978)
                     /locus_tag="VFM00_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496734.1"
                     /translation="VTAIFTLKDKKGKSFTDGIVDTDPTNIANIVQMGYERWVNDTYW
                     IIMPFKLRDPGTHLKYLRMQKRGGQDYDVLQLSFDRGVGLTSGDHYTLYVNRSTHLLD
                     KWDMMLEGKKPPPSTSTWEQWTPIGPVKLALNHRLQGKPVEIRFDNVAVPQTMDPSVF
                     TNARVRG"
BASE COUNT         2082 a         4946 c         4872 g         2078 t
ORIGIN      
        1 ctctccgacg aggtcgcgct ggtcacgccg cttctcctga tcggcctgga ccgctgggga
       61 tcgccgcgcg tggccgagcg ggggcgccgc acggtctact ggggattcac cgccgtcgcc
      121 gtcgcgggcc tgctcgcccg gctcggagcg cactcgctcc ggctgaacga gccgcacgac
      181 caggtcgcgg ccggcaccgc tttctgggcg ccgctgatcg cgacaggcga gtacctgcgc
      241 gcactgctcg tcccctaccc cttgaacgcg atgcgctcgc tcaccacccc ggacgcggca
      301 tcgtggggac ttcgtctcca ggcgctcggc ggcgtggccc tgatcgcgct gttcgtgtgg
      361 ctccgccgca aagatcccct cgcgcggacg ggcgctctgc tcctcttcct cccgctcctc
      421 cccgcgctgc cgatcggccc cttccagggg accttcgtcg aggagcgcgc ggcctatttc
      481 gcctcaaccg gcctcgtctt cctgatggcc tcgctcgtcg ccgggatcgc ggggcgcccc
      541 cgcgagacgc gcatcgcggc ggcggcggtg atggccgcgg tcgtgctggg cgcgggtgcc
      601 ctcaccatga accggctgcc ggtctggtcg agcaacgtgg cgctgctgga cgacgccgtg
      661 cggcacgacc cgcgcgatcc cgcggcgcac gtcgcgctgg ccgaccagta catcgccgag
      721 gggaactacc atgcggcgct cgcttccctg gaccgcgcga tcgccgtcga ttcgacccgc
      781 gccctggcgt ggcaccgccg gacgctcctt ctcaaccgga tggggaagct ccccgaggcc
      841 gaggcgtcgg gccgtcacgc ggtggcgatc cagccctccg aggcgatctt ctgggccaac
      901 ctgggcgaca tcctgacccg cgaggggaag acgcacgacg ccaacgcggc gaacggccgc
      961 gcggtgaccc tggacccgaa gaaccccgat aactggtaca actatggcgt gtcgctcgcc
     1021 gcgtcggaca gccttcccca ggccatgcag gcctatcgcc gggcgatcgg gctcaaccct
     1081 ggccacttcc aggctgtcaa taacctgggg gcggccctcg ccatctccgg ccggatcgcg
     1141 gaagcgcgcg acgtgtacat gaaagcagtc gagctcgagc ccagctccgt ccaggcccgc
     1201 atgaacctgg cgctggccta tctgcgcctg ggcgacgtcg cgggcgcctc cgcccagcgc
     1261 gaagcggtcc agaagctgga ccaggcggcg gcgatgcagc tggtcgagat gatcaagcag
     1321 gtgcaggtag gcaaaggggc gacccgatga cccccgccct cgtccgccgc tcgagccgca
     1381 catcatgacc cccgccctcg acacccgcct cttcgacccc agccggatca acctcctcgt
     1441 cggcctcggg atcttcctca tcttcctgat catcaacatc tcgcgcgccc ggcgcggcgc
     1501 gggcatgttc atccggccct tagccgggct caaggcggtg gaggaggcga tcgggcgcgc
     1561 gacggagatg gggcgccccg tgctctacgt tccgggtctc ggggacatga gcgaggtggg
     1621 cacgctcgcc tcgatcacca tcctgagccg cgtcgcgcgg cggaccgccg agatgggatg
     1681 cgacctggag gtgccgggct acgatcccgt agccgcgacg atcatggagg gcgcggtgcg
     1741 ccaggcctac atcgaggccg gccgcggcga cgccttccgt cccggctccg tgcactacgt
     1801 gagcgcgcag cagttcgcct acgtcgccgc ggtgaccggc tacatgcttc gcgcgcggcc
     1861 ggccacgaac atcttcatgg gctccttcta cgccgagtcg ctcttcctgg ccgagaccgg
     1921 caacctgacc ggcgcgatcc agatcgcggg caccgatcag gtcacccagc tccccttctt
     1981 cgtctgcgcg tgcgactaca cgctgctcgg cgaggagctg tacgcggcca gcgcctatct
     2041 cggcaaggag ccgatccagc tcgcgacgat cgcggcgcag gactgggcca aggcgctcgc
     2101 ggcggccacg gtcgtggtgg cttccatcct gatggcgctc gggcatcccg aagtgggaaa
     2161 gctcctccac acccagtgag cgactgagcc catgcgcttc ggcccgctcc tcgtcacgtt
     2221 cgtcaccggc gcgctcctcg tcgccttctt cttcgtgccg catcacgccg cccaggaagc
     2281 gcagagcgtc atgctcgact gggcgaacgt ggtctacgcc ttcgcgctcg tgctgggctc
     2341 ggtctcgctc tggaccgcgc accagaagaa ggtgacgacg cgcgcgccgg gatggatcta
     2401 cagcgtggtg acgctggcgt cgctcgcgtt cgtcaccatc tggggctgcg tccagggggt
     2461 ggaggaaggc acggcggtga acgccttcta caacacggtg aacgcgccgc tcgcctccac
     2521 gatgttcgcg ctcctcgcgt tcttcatcgc ctcggccgcg ttccgcgcct ttcgcgcccg
     2581 caatgccgaa gccaccctcc tcctcgtgac cgcgatcgtc atgatgatcg ggcgcgtgtc
     2641 gatcggcgag tacctctggc ccggctttcc gaaagtgacc gagtggctcc tcgacgtgcc
     2701 gaacctggcc gcgaagcgcg cggtggcgat cggggtggga ctgggcgcga tctcgaccgc
     2761 gctcaagatc atcctcggca tcgaccggtc gtacctgggg cgctcatgag cgcggcacgg
     2821 aggtcgtcat gaacgggccg cggcagagca tctggacccg cctcctgcgt ctcgaccggc
     2881 ggatcatctt cgtcctgatc gcgctcgcca cggcgatccc gctccttcgg cccgtgaacc
     2941 ttccgatcgc ggtctcgccg cgcgtgcgcg ccgcctacga cacggtggac aagctgcccc
     3001 gcggctccta cgtgctcatg tcgctcgact acgagccgga catcatggcc gagctccagc
     3061 cgatggcgaa ggcggtgctc cgccactgct tccggaaggg attgaagccg gtggtcctga
     3121 cgctctatcc cgcggccccg gggctgatcg aggaggcgct gcgcgtgagc gccaccgccg
     3181 agggcaagaa gcggaacgtg gactacgtgt tcctggggta caagtcgggg agccagtcgg
     3241 tgatcctggg gctgggcgag agcatccgca ccatgttccc caccgatttc tgggggaccc
     3301 cgttgaacca gatcccgatg atgaaggggc acaattcctt cgccgacttc ccgctcgtga
     3361 tcaacctttc cggctcctcg atcgccgact actacgtccg gatcgcggcg acgcgctacc
     3421 gcaggccgat gatcctcggc tcgacggcgg tcatgacggc cgactactac ccgtatctct
     3481 ccgcggggca gatcctgggg ctgatcgggg ggatgaaggg cgcggccgag tacgagcggc
     3541 tgatggacct cttcggcgac gcgcggcgcg ggatggacgc gcagagcctg gtgcacgtgg
     3601 tgatcgtggt gctcgtgatc ctgggcaacc tggcgctgtt ctcgtcggga gggctgcgcc
     3661 ggtcatgatc cccgtcacgc agaagatagg gccgcgccgg tcatgaatcc cgtcacgcag
     3721 aactggatcg cggccttcct cactttgtgc gtgttcagct tcctctatcg cgacaatccc
     3781 ttctaccgat tcgcggagca tctcttcgtc ggcgccgcgg ccggctatct cctggccgtg
     3841 cagtatcaga acgtgatcaa gccgaacgtg atcggcgaga tgaaccaggg gaacatgctg
     3901 ccgctggttc cgctggcgct ggggatcatc ctgctggggc ggctctggcc gcgcacgtcg
     3961 ggcatcgcgc gctggtcgct cgccttctac gtggggatct actcggggat cgcgattccg
     4021 tcctatctgc aggcgcagat cttcgcgcaa ctggccgacc aggtgaagcc gttcgccccc
     4081 ggatggggcg cggtcaactc ggcgctcatc ctgatcggct tcatcaccgt gctttcgtac
     4141 ttcttcttct ccgccgccca cagggggctg cacggcctgt cggcgcggat cggcatctgg
     4201 ttcttgatgg tcgccttcgg cgcgtcgttc ggctacacgg tgatggcgcg cgtctccttg
     4261 ctcatcgcgc gcatgcagtt tctactgagg gactggctgc acctgctgcc cccggcttag
     4321 ccaggcggga cggccacccg tctagccagg ctgaacagcg ggaccctcca ggatccgaaa
     4381 cgccgactcc agcgcctggt ggtccccgac cagcaccatg atgtctccga tctccagctc
     4441 gaggtcggct tccggcgaga tcttcggctt ccccgcgcgc acgatcgcga tcaccagggc
     4501 gcccgtgcgg ccccgcagat cgagggacgc cagcgtgtgt cccgccgcgg gcgagcccgg
     4561 ctcgacgaag aaggtgtcgg tcaccgcgcc cgcgatcatc cgctcgatcg agtgggtcag
     4621 ctccaccgag acgtcgccgc cccggaggaa ggcgtagccc tcgcggcgga gcgcgccctc
     4681 ctgcagccgg atcacgtttc ccggcacgtg gtagcggcgc agcacgcgcg tgacgatggc
     4741 gatcgaggtc tcgaactcct cggggatcgc ctcgtcggcg cccaggcgac gcagctccgc
     4801 gacctcggcg accaagcgcg tgcgcgcgat gatgtaggcg ccgggattgg cgcggcgcac
     4861 ctgggacacc gcgtgccgcg tggccgacgc gtcggagatg gcgagcacca ccaccgcggc
     4921 gcgctccacc ccgcaggcgt gcaggctctc ggggcgcgag acgtccccga actggatcgg
     4981 aacgccctgc gcgcgggccc ggcgcacgtt cgaggggtcc atctcgagca cgacgtagtg
     5041 gatccccagc tcggccagcg cgtgcgcgac gttcctgccg ttcaagccgt agcccgcgac
     5101 gatgacgtga tcgcgcagcg cgcccgcttc cgttcccgtg ccggagcctt cctcgcgcgc
     5161 cctccccccg aaccgcgcca cccccgagca tgaggcgctg gcaccggccc ccgtcgcccg
     5221 cgcggcctcg cgggcgccca gccacgatcc gaacgggacc aggaacggcg tgatcaccag
     5281 gctcagcacc gcgaccgcga agaactcgcg gctctgcgcc gccgaaagga gcccgttccg
     5341 gatcccgacc gaggcgagca cgaaggagaa ttctccgacc tgcgcgagcg agatccccac
     5401 gatcgcggcc acgcgcaggg ggaagcccat caaacgcacg atcgcggccg cgacgatcgc
     5461 cttgagcgcc acggtcagga agagccagcc gccgatcgag gggaggttcg cggcgacgaa
     5521 cgcgggctcg aggagcatcc cgatcgagat gaagaagagg ctggagaaga gatcgcggaa
     5581 gggcacgatc tcggagacga cctggtggga gtactccgat tcgctcacga ggagccccgc
     5641 gaggaaggcg ccgagcgcgg gcgagagccc gaggcgctgc gtaaggagcg ccgatcccag
     5701 gcagaccgcg accgccatca tgacgtagag ctcgcggacg ccgctccgga tgacggcgtt
     5761 cacgatgcgc ggcatgagcc agcgcgcggc ggcgatcact agacccgcga tcgccacgcc
     5821 gagcaggagg cgcgcggcga gcgagagcgt cccgcttccc gagagcgcgc cgctaccgcc
     5881 cgagagcgcg ccgctaccgc ccgagagcgc gccgacggcc gccgctccgc cgtccccgac
     5941 ggcgccggcc gcgcccaccg ctcccgccgc cgcggcgagc gcgggcacga gcagcagcat
     6001 cggcaccatc gcgatgtcct ggaaaaggag gatgcccacg agaagacgcc cctgcggcgc
     6061 gtgcgtctcg ccgcgctccc ccaggctccg catgaccatc gccgtggagg agagggtgac
     6121 gagcattccg gcgaagacgg cgccggcgag ccgcgcgtgg atcggcagga ggagcaggac
     6181 gccggtcacc gcggcgatcg tcgcgaccac ctggagcggc cccgccacga ggaaggcgcg
     6241 gccgatctcg cgcaggcgcg cgagcgagaa ctcgagaccg acgatgaaga ggaggaccga
     6301 gacgccgacc tcagcgagag cgctgacatg ggcggtgtcc gcgatccagc cgagcgcgcc
     6361 ggggccgatc aggatgcccg tgagcatgaa cccgaccgcg gcgggaatcc tgaagcggcg
     6421 gctgggaagc agcacgacga gtgccgcggc cagcgcgacg accaggtcgc ggaggaaggt
     6481 gctctcgccg tgcatcaggc ccggcccatg acggtccgac ccgtcacggc cttccccgcc
     6541 tggggcgcgg cgctcatccc agcgagcgga gcgcttcgcc cagccgcgtg atctccgcgc
     6601 gcgtgttgta gtggcagagg ctcacgcggg cgaccgcgtc gcggcccacc gcctccagca
     6661 ggcgcggcgc gtacatgtgc ccctcgcgcg cgtggatgcc gcgctcggcc aggcgctcca
     6721 cgatcgcgcg cggcacggac cgttcgatcg tgaacgccac ggtgggaacg cgcgcctcga
     6781 cgcgcgcggg gtcggcatcg cccaggatcg agagcccggg gacccggcgc agctcctcga
     6841 ggagcgccga cgccagctcc gtctcgtagg cgcggatccg ctcggcgccg atctccatca
     6901 cgtagcggag tgccgcggcc atgccggcca tcgcctcgaa gttctgcgtg cccccttcat
     6961 atccgtaggg ggcttccgcg gggatgaaga aatcgtgcac cggggtcagg cggcggtacg
     7021 agtcggcccg tccccacaca aagccgatgt gggggccgaa gatcttgtag cccgagaagg
     7081 cgaggaagtc ggcacccagc gcctggacgt cgatcggtcc gtgcggcgcg aagtggacgg
     7141 cgtccacgaa cgccagcgcg cccgcttcgt gcgcgacccg cgcggccgcc gccacgtcga
     7201 cgatccggcc gatggcgttc gaggcgagcg gcatggcgac gagacgcgcg cgtccgtcgg
     7261 actcgcgcag gagctcccgg agatcgtcca ggtcgagccg cgcctcggtg ccgcgcacct
     7321 tccagacgag cgggaccacg ccgtcgcgct ccagccgaag ccacgggccg acgttcgcct
     7381 cgtgatcgag ctgcgtgacg atgacacggt cccccgcgga aaagagcggg cgcaccgctt
     7441 cggctaccgt tcgaatgagc gacgtggaat tcagcccgaa gacgatctcc tcgggcgagc
     7501 gggcgttgag gaaggcggcg acggcctctc gcgcggcggc gatctgccgg tccacctcga
     7561 tggagcgcgc gaagagcccg ccgcggttga cgccgcgacg gacgagatgt tcgcgcatcg
     7621 cctcgatcac gtcatcgggg acctgggcgc ccgccgcgtt gtcgaagaag atctcccggt
     7681 cgccggcctc gcgcagggcg ggaaagcgag cacgcagcgc gtccaccgga aaatcagatc
     7741 gcgacataga cgtcctcccc ctcgaccttc acctcgaacg accgcaccgc gaccaggtcc
     7801 gcctcgggcg acttccccgt cttcacgctg aacttccagc cgtgccacgg gcagcgcacc
     7861 acgtcgccgg tcaggagccc ctcgccgagg tcgcccccca tgtgcgggca ttggtttcgg
     7921 atcgcgtaga ccgcgccctc gcagcggaag agcgccacct tgatcggccc cgcccagacg
     7981 atcagcccct tcccctcctc cacgtcggac gcgcgggcca ccttcacgaa ctgggtcatg
     8041 aagcccgcag tctatcagaa ggagaactcg aatccggccg ccggcatgcg ggggaagagc
     8101 tcgaccgcct gaaggcgcgg cgccgcgtca gcacccgggt cgaagacgta cgtgtactgg
     8161 tgcacgttca cgcgcccggt ggcgttgatc agctccacgt aggcctgccc gcccgtcggg
     8221 ccgaaggaga gcgggtgcga gaggcggaag tcgaggcgga agtaggcggg atagcgcgcg
     8281 cccatgaacg tcccgaagcc gcgcgtccac gtgcccgtgg aatcgtcacg cgcccacacc
     8341 gacgcggtgt agggcgagcc ggtgtgatag cgcgcgagga cgttcaggtt ccacccggcg
     8401 gccggcctca cgttgaggga gagcgccgcc gcgtggcgct ggtcgaagga tcgggggacg
     8461 atcccctcgg gcgcggtcca gagggcgcgg ctcagcgtgt agccgagcga cgcgtcggcg
     8521 cgcgcccagg agggcgtgcg cagcgagagc tccacgccgc gcgccgtacc gcggtcgagc
     8581 gcgaagggag gggacggctg cgcggtcacg tcctgcgccg ggagcccggc gccgcgctcc
     8641 ttgcgaaggt atccctcgac gcgaaaccgc gcgcccgccg gcgcgacttc gaacccggcc
     8701 gcggtctccg cggcctcgcg gtttcggccg agaggcaggc tctcgcgttc caggttgttc
     8761 aacagcgaag gctggcgcat caggcccatc gacgcccacg ccgatcccgc gctcccaagc
     8821 cgcgcgagca ccccaagccg cggcgagacg aacgcgaacc ccggcgtgcc gggaaggggc
     8881 gcgccgtcct ggcggagtcc ccaggcgtag aggtcccgcg agacgttcac ccccgcggtc
     8941 accgtcgcgc gcgagccggc cgacgcccgc gcggaaagat acgcttcccc tcggcggcgg
     9001 atggcggtgc ccgcgatcac gtgctccact tcctcctggt agcccgacgt cccgagcgag
     9061 ccgcggatgc cgtccagggc cagccagcca tcttcgtact ccgccgcggc accccactcg
     9121 agcgctttgc cgcggccgag ggagagctcc ccttcgagcc gcgcgcgcaa cgcgcgcgtg
     9181 acgctgtcgt cgcgcccgct ccgccccacc agggtcgcgc ggtggaaccg gtcggcggag
     9241 aacaccagcc ggtgctgcac gcgctccgac ggccggtagg accaggacgc ccccagcgtg
     9301 aggttccgca cggagccgtt caggtcgttt tcgtcgtagg gcatgtcgta gcggagccgc
     9361 tcgcgcgcgc ccagggcgag gaacgtcacc tcgtggccct cggccgggcg gagccgcgcc
     9421 tcgccgatca ggtcctggaa gtcggggacg atcgaggcgt cgcgatccag gcgatgcagg
     9481 cgcccgatcg cggcgaggag cccgtggcga atgccgagaa ggtaggagcc gccgcccttc
     9541 gtggggcccg cgacgagggc gcgaagctgc gtgggaccgg cacccacctg gtagcgcggc
     9601 ccctcggggc ggtcgctcgg cgattggatc tcaagcgccc ccgagagctc gcggccgtag
     9661 cgcgcgggga gcccgccgcg cgagagctgc atcgtctgca ccacgtcggg actcaccacc
     9721 gagatggctc cgccccaata ggggatgtgg tagggctcga gcaggtcgaa tccgtcgaag
     9781 cgcacgagcg tctcgtcgct ttccccaccg cgtaccagga aggaggcggc gtagtcggag
     9841 ctggcgacgc ccggcagcgt ctggacgacg cggaaggcgt cgtcggcggt ggccgcggtg
     9901 cgcccaatct gctcgcgcgt gatgaagtcg gcgcgggggt cgatcccctg gaagcgcggc
     9961 gcgcgcacgt cgacgccggg gagtgagtaa cggatggagt cgggcgcggg cgggatgtcc
    10021 gaggaatcgg gagcgggacc ggaggagtcg ggagcgggac cggaggagtc ggcgacggcc
    10081 ggcatggtgg aggcgcgagc ggtcgcgacc actcccagga agaccgcggc gagaagggcg
    10141 gcacggatga gcggcatggg atgagcccct tccacggcac gacggaaggc ctcatcgccc
    10201 gggcgaagcc gctatgccgg cgccgccccc caggagcagg tgccgcgcgg gacgcggcgc
    10261 tcgctccccg gtctcacccc aaaacgaaga ccggcgcccc gtttccggag cgccgatcga
    10321 ttgtaccaga cttttcgcgg ccgtcgtggc ggccgtgcgc cttagtggct tccgcagcct
    10381 ccaccgcttc cgccgcagcc gcatcccccg ccgggcgcgg ccccttcgct ctcttcggtc
    10441 gcgaaggaag atccgcagcc gcagctcttc accgcgttcg gattgttgat cttgaagccg
    10501 ccgccctgca gcgagtcgtc gaaatcgatc gacgcgccct cgaggaactg gtggctctgg
    10561 acatcgacga tcaccttgac gcctccggcc tcgaagacga ggtcgtcggc cgtggcgggc
    10621 tcgtcggcga tctgcatgcc ataggagagg cccgagcacc caccgcccgc gacgaagacg
    10681 cgcacgcctt cgaacgtgcc tccctcgcgc tccatgatgg tcctgatctc ggacgcggcc
    10741 ttttcgctca acgtgatcat gcggaactcc ttaggtcgta acgacgagtt tcggccggac
    10801 ggccgaccat acgcttggac gccgccctcc gcgaaacgat gctatctctt gtatcggtaa
    10861 atgataggcc tgccttgagg ggctgtcaat gtgacctcac cgccgcggct tgtccggggg
    10921 ccgcccctgc tgaaggatcc gcagcatgtt cgtccccccc gagcgcgtcg tcgtccccgc
    10981 catcgccacc acccagtccc cccggcggat catcttccgc gtgatgagga tgcggaggcc
    11041 cgcctcgacc atcctctcgg cggtgcgcca gcggggtacc tggaccgcac tcacccccca
    11101 ggcgagcgac aggcgccggc aggtcgactc gagcggggtc agcgcgaaga tcggcgcctg
    11161 cggccgcgac ttggagacga gccgcgcgga ataaccggtg tgcgtgaaga tggcgagggc
    11221 cttggcgcgg atctcgcgcg aggcctggta ggccgtgtgc gccagagcgt gggtcggtga
    11281 ggggagcgcg ccgccgtcgg agcggcgcgg cgtgcgcttg gtggccgcga actcgtcggc
    11341 ctcccccgcg atccgcgcca tggtgcgcac cgcgcgctcg ggatagcgcc ccaccgccgt
    11401 ctccgcgctc agcatcacgg cgtcggtccc gtcgaagatg gcgttcgcga cgtcggaggc
    11461 ttcagcgcgc gtcgggctgg aggaggtcac catcgactcc agcatctggg tcgccgtgat
    11521 cacgatgacc tcgagcgcgt tcgcgcgctc gatgatccgc ttctgcaaga tcggcacgcg
    11581 ctcgggggga tactcgacgc ccaggtcgcc gcgggccacc atcacgccgt ccgccgcttc
    11641 caggatcgac tcgagattct cgatcgcctc ccggcgctcg atcttggcga tgaccatcgg
    11701 cgggcgctgg gcgcgccgca gcaggcggcg caggccgatc aggtcctcgg cgctccgcac
    11761 gaaggagagc gcgacgaagt cgatcccctc ctcgatgccg aacgcgagat cgcggcggtc
    11821 cttcgccgtg agcgccggcg cggagagccg cacgccggga aagttcacgc ccttgtggtc
    11881 ggagatcgcg cccccggtaa ccacgcggca gcgcaggccg cccgcggcct tccggaccgc
    11941 cttgagctcg accttgccgt cgtcgatcag gacgcggtcc ccgacggaga cgtcctgcat
    12001 caggcccgaa tagtcgatcg ggatccgctc ggcggtcccg atcccgccct cgctggtcag
    12061 gagcacctcg gcgccggcgg cgatccggat cgagccgtcc cggaacgatc ccacgcgcag
    12121 gcgcgggccc tggaggtcga ggaggatccc gacggtgcgc cgcgcgcggt gggcggcgag
    12181 ccgcacggcc cgcatggcgc ggcggtggtc ggtgtaggtg ccgtgggaga agttgatgcg
    12241 ggcgacgtcc atgccggcgc gagccatgcg cgcgagcacg gcgggatcgg cgctggccgg
    12301 ccctaccgtc gcgatgatct tggtgcggcg caccgcttac ggggcgctag acggcgaggg
    12361 tcgcgagcac ttcgtcggcg tgcccctcga ccttgacccg gcggaatacg ttctcgacct
    12421 tcccctcgcg atcgaccacg aacgtcgtgc gctcgatgcc ccagaacgaa cggccgtaca
    12481 tgttcttctc cttgtagacg ccgtactggc ggcagaccgc cgcgtcgacg tcggacagga
    12541 gcgggaaggg caggttgtac ttctgcgcga agcgctggtg gctgagctcg tcgtccaggc
    12601 tgatccccag cacgatgccg tccttggcct ggaagcgggg caggttgtcg cggaaggcgc
    12661 aggcctccat ggtgcagccc ggcgtgtcgt ccttgggata gaagtagagg atgacgcgct
    12721 tgccgcggaa ttcgtgcagg gtcaccttgc ggcccagcgt gctcggcagc tcgaagtcgg
    12781 gtgcgggcat tccgaccgtc aggttctcgc cgggccagtc gggtccaccc ggcttggcgc
    12841 cgcgccgctt cggaatggga agctcgaccg gcggttgcgg cggaccgact tcgaattcca
    12901 gcgcgccctg ctgaccgggc actttcccgg cggtcttgcc cttggcggcc gcctggcccg
    12961 gagcggcctt ggctgccgcg gccggccgtg ccgttctcga gctggaggag gcgggcgatt
    13021 tcgtgccgga agccgcccgg gcgggagctg cggcccgggc cggggccttc ttggtcgcgc
    13081 gcttaggagc gacccgaccc ttggccgggg ccttcttggc gggctggcgc ttgacctgct
    13141 tcttccggaa cgtggcggct ggacgtttcg gcatcttctt cgctggtttc ctcttactgg
    13201 attttgcgct cttgcgagcg gaccctttcc ccgccattca ggaaccccct tggcacaggg
    13261 atacggattg cggcaccggt catcgggcat cctaaccgct gtgacggcac cgcgcaagag
    13321 cggtcgagac gattccgtgc caagaagcgc gatttcacgc ggggggatcg gagggggcgg
    13381 gagagaagga cgagcggtta cgagtcaggc gggggcgtcc gcgaggacgg catcgggcgc
    13441 gcgcagaagc tacgcgaagt cagcccgacc cgggctaacc ccggacgcgg gcgttcgtga
    13501 agacgctcgg gtccatggtc tgcggtaccg ccacgttgtc gaagcggatc tccaccggct
    13561 tgccctgcaa gcgatgattc agggccagct tgaccgggcc gatcggcgtc cattgctccc
    13621 acgtggaggt cgagggtgga ggcttcttcc cttccagcat catgtcccac ttgtcgagca
    13681 ggtgggtgga ccggttcacg tagagggtgt agtggtcgcc ggacgtgagc ccgactcccc
    13741 tgtcgaagga gagctgcagc acgtcgtaat cctggccgcc gcgcttctgc atgcgaaggt
    13801 acttgaggtg cgtgcccggg tcgcgaagct tgaacggcat gatgatccag taggtgtcgt
    13861 tcacccaccg ctcgtagccc atctggacga tgttcgcgat gttggtgggg tcggtgtcca
    13921 cgatcccgtc cgtgaacgac ttccccttct tgtccttcag cgtgaagatc gcggtgac
//
LOCUS       DASSLZ010000011         8914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_512298, whole genome shotgun sequence.
ACCESSION   DASSLZ010000011 DASSLZ010000000
VERSION     DASSLZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512298"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1422
                     /locus_tag="VFM00_00390"
     CDS             31..1422
                     /locus_tag="VFM00_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET7496735.1"
                     /translation="MLALLSLVLLPIPSAAAFSVPANTWVAQKAPTQVLPSGLAGSYQ
                     GRGWNHLRYDSVSGRMVLFDGYNEPPAYPLGNIYANSLWSYDPVKNQLSLEKLDHWTH
                     TSTGTGPLPENVADPTPYDRHSYACIVYVASHDAVYLWSGANSNIPDGYVGDMWSYSF
                     PGHAWRPIAGPHPFTVFEQAMAYDPFLEKLILFGGSDRGYHDGDKTYVFDLKSELWTD
                     VAPVTVPAPRAGQTLTFDSARRVTWMFGGAINGQPTNELWQYDARANTWVQVAASGDT
                     PSPRRFANMAYDSTHDVVLLWGGVTGSNAALADTWVLHPQSRTWEKLSPTRSPDPLVN
                     YAEDMDYDPVNDVFVMNQSGKFWLFRYAGEPVGVATTQAHGLQLRLLSANPARGQARL
                     SFTLSREADVRIDMVDSLGRRVATLVQGRYPAGEHQVDWAGGTSRRTASGIYYVRLTT
                     EGRVLTRRVALVR"
     gene            1574..2476
                     /locus_tag="VFM00_00395"
     CDS             1574..2476
                     /locus_tag="VFM00_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7496736.1"
                     /translation="MFYLEMSPLRSALTGLLAALGQEPTARFATVRHDPDLYAEPPGG
                     AVTFWGHACVYIDVRGRGIVTDPVFGRRYSPLNGRRIPSPPPSAYEETRVILISHAHY
                     DHLQPRTLARFPRRATILCPPPSERYVRNLGPRVRVMRVGEEYTIRGASIVAVPADHP
                     GGRYSHTAEADGRALGYVIRTEIGTLYYSGDTDYFPGIETIGRSYKPDLALLNVNAHL
                     RPADALRAAEALGSPPVVPLHLGAYGGRAARRGHRWHREFLEMAGPLALPLAVGQSVI
                     LNGSVSGPVVASRPALRLVRDFTA"
     gene            complement(2543..3289)
                     /locus_tag="VFM00_00400"
     CDS             complement(2543..3289)
                     /locus_tag="VFM00_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496737.1"
                     /translation="MRRIIVPSLTAALVLAFTAAAAAEGNDAKQGGVVPLGATSVPEP
                     VPATQTTTSGEATTTATAPAPDAAKNEARLKAIRDRAAKMPATDREEIQKKLEGNIKD
                     VDVEAVANGDATVADRFAAEFGVTSEALLAERTQYNVGWGEVMIAHTLLANAPSGTTI
                     DQLYALHADGMGWGQVAYGMGLRVGSVAAAVKTETLVARGTMKADGKPAKIGANTRVA
                     STGSTHAAAGQTRAGVASSAAMGVGAKVTK"
     gene            complement(3351..4112)
                     /locus_tag="VFM00_00405"
     CDS             complement(3351..4112)
                     /locus_tag="VFM00_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496738.1"
                     /translation="MRRPAPPTGSTLLLLCAVLLTGGRLGSACASPLSAGAGVDRQAG
                     PGHQTYTAGLLFGTVSVPLADATLALIRYDDSVTGYGNALFANAGVGVPGLARIRAIG
                     ARSFGDGGYRAWKLRAGPEWSLGLVTAGVYGLHQEDNVSARLNAIGVEVGTSVVPGLA
                     LRAGGMAGKREGDANASQASGSFVWGAFSHVQILGELDVGRGVATSTTSTSGGGPLHR
                     LSLAGGLGAGSAGERTVTDTSVETVGLLGVRFLIP"
     gene            complement(4241..8023)
                     /locus_tag="VFM00_00410"
     CDS             complement(4241..8023)
                     /locus_tag="VFM00_00410"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET7496739.1"
                     /translation="MTPRRRRYLVAGLSAVSLVAVVLAGSWWLFFTQPGAKWGFERLG
                     SFFPGRLDVRDMTGPLRGPLIVRHFTYRNDRLAITADSVFIDWRLRELWGRRLDIHNL
                     YAKNARIVVGGPGDTPEAQDSLRGLPDLNLPVTVIVRNGVVNGLTLARPGSDSGLVID
                     QVRLDARSMRRDSVRVNALMVRSRTIDLDFSGSALPRGAYPLALRGKWTYRPAGSAPI
                     QGDGTLSGTLEALRVRQSLKGPFDAQVDVTLIRPLRRQARFRGDVEFSRLTPRDFGPA
                     WPQGIFEGHVALDGDARAATAQGRIRGTTEALGPTAADFRVRRLERGDWQIDDLAVTQ
                     PGAPGRVTARGTLSGDSANPRFDLRAEWAAMGWPLRGKRWVESERGSARVRGTMADFD
                     IHSEALLAGRNVPPGHWTLDGRGGNGRLAVHTVIANIFGGRITGSGSVAWQPGVSWRL
                     SFDGRGIDPGTVWRAYPGRLDFAGRSEGVNGPSGPSGRILVSRLDGTVRNQPVTAAGT
                     LVANRGQYRLDRATATWGPDSVQASGAFGRSFDLAWRVSAPKLGAVLAQASGSLEAQG
                     TIRGTRAHPHIAGTVTGDSLFFANEHAGALRADGDVDLRPGGIVRLDLSASRVSAGIH
                     AADRMIVTARGTRERHEWRASVAGRADSTVAVLAGGFAAGGWRGTIARLDLVNPRSGN
                     WSLAAPARLTAVNGRVAVTEFNWQSGTSRIVATADWARRGPWHADARLEQVNLTLLQP
                     GLPPRLRLSGTMAGHVSAHGTAEGQLFADVDVVPGPGEILHETAEGLWVPTRFQNARL
                     TAVADGRRVQTTLAADLVNVGTVRGNLGWPAYGSFAEGTSRPIDGRLALHLRDLSLAQ
                     GLTPEVDATSGALDADLAIAGTVQRPFLYGPLTLRNGSANIPRYGLQLRDMNVEGRGS
                     LGGAIELHGSVRSGSGTLAIDGTAAVARGARPVATVTVKGNRVQAMNTRDMQVVASPD
                     LKFALNGNRLDITGEVDVPEGTITIGRADERRLVKTSPDVVFTGADTLSEMPTEVHTR
                     VRLVMGDKVRVTGFGLDVRPTGSVVAIDTPGLPTLGNGRLDIKDGTYHIYGQDLAVET
                     GTLIFAGGPITDPAVRARATRTAADGTVAGFIVSGTVSKPDVQVFSEPAMGQSEALSY
                     IMFGKPIESANLSEGQMASTLASTMGVPGTNLLAQGVASELGIEQARLDVGTSLQSTS
                     LSLGTHLSPKLYVSAGMDVFQSTSSLRLRYILNRIFTIEAETARQNRVDILYTVEP"
     gene            complement(8020..>8914)
                     /locus_tag="VFM00_00415"
     CDS             complement(8020..>8914)
                     /locus_tag="VFM00_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET7496740.1"
                     /translation="DDGARVRLVTEMRRLNRAGHRAQINLQYGSRDRHAIGEYFIPWP
                     HARTDLLTLSTGYQELATATFHSKVFRSGISESRLLGKWRVIPALNYRRENFQVGVDS
                     GIVKTLVPEGTWTRVRADDALFTKNGDRLFLNARLGDRRVVSDVSFQQVRGDAKLIHA
                     FGPRTRGIARIEAGATHTADFRSLPPSLRFFAGGTNSVRGYSYNSLGPRDELRHVIGG
                     RYLLTGTVELNQFFLPRWGGAVFFDAGNAMNSWRLPVKRGAGFGLRWISPAGLVRADV
                     AWGLDRRGTPIQVHLAMGAEL"
BASE COUNT         1277 a         3187 c         3130 g         1320 t
ORIGIN      
        1 aggcgccctc atacatcgtt cgcaggagct ttgctcgccc tcctatcgct ggtccttctc
       61 ccgatcccgt ccgccgccgc gttctccgtc cccgccaaca cctgggtcgc tcagaaggcg
      121 cccacgcagg tcctgccctc cggacttgcg ggctcgtatc aagggcgggg atggaaccac
      181 ctgcgctacg actcggtgag cggccggatg gtgctcttcg acggctacaa cgagccgccg
      241 gcgtatccgc tggggaacat ctacgcaaac tcgctctgga gctatgaccc ggtgaagaac
      301 cagctctccc tggagaagct ggatcactgg acgcatacga gcaccggcac cggacccctt
      361 ccggaaaacg tcgccgaccc cacgccctac gaccggcact cgtacgcgtg catcgtctac
      421 gtggcctccc acgacgccgt ctacctctgg tccggggcca acagcaacat ccccgacgga
      481 tacgtcggcg acatgtggag ctactccttc ccggggcacg cctggaggcc gatcgcgggc
      541 ccgcacccgt tcaccgtctt cgagcaggcg atggcctacg atcccttcct cgagaagctg
      601 attctcttcg gcggatcgga ccggggctac cacgacgggg acaagaccta cgtcttcgat
      661 ctgaagagcg agctctggac cgatgtcgct ccggtgacgg tcccggcgcc ccgcgccggc
      721 cagacgctca cgttcgactc cgcccgccgc gtgacctgga tgttcggcgg ggcgatcaac
      781 gggcagccga cgaacgagct ctggcagtat gacgcgcgcg cgaacacctg ggtccaggtc
      841 gccgcgtcgg gcgatacccc cagtccgcgc cgctttgcca acatggccta cgactccacg
      901 cacgacgtgg tgctcctctg gggcggcgtc accggctcga acgcggcgct cgcggacacc
      961 tgggtgctcc atccccaaag ccgaacctgg gagaagctct cgcccacgag gtctccggac
     1021 cccttggtga actacgccga ggacatggac tacgacccgg tcaacgacgt cttcgtgatg
     1081 aaccagagcg ggaagttctg gctcttccgc tatgccggag agccggtggg cgtcgcgacg
     1141 acgcaggcgc acggcctcca gctgcgcctg ctctcggcca acccggcgcg gggccaggcg
     1201 cggctgagct tcacgctgag ccgcgaggcg gacgtacgga tcgacatggt cgactccctc
     1261 gggcggcgcg tcgcgacgct ggtccagggg cgctaccctg cgggcgagca ccaggtggac
     1321 tgggccggcg gcacgtcgcg ccgcacggct tcggggatct actacgtgcg gctcacgacg
     1381 gaggggcgcg tgttgacgcg gagggtggcg ctggttcgat agagccgcat ggggagcgag
     1441 cagcgctttg gaaacgggac ccgtcgattt cgagtaatgg tttgcaggag gattcccgaa
     1501 agagtagaag agagcggggc ccggctcatg gggggtgagc accgggcatg cgaggagcaa
     1561 ccttgcgcgg acggtgttct acctcgaaat gtcgccactg agatcagccc tcaccggcct
     1621 gctggccgcc ctgggccagg agcccacggc ccgattcgcg acggtccggc acgatccgga
     1681 cctctacgcg gagccgccgg gcggtgccgt caccttctgg ggccacgcct gcgtctacat
     1741 cgacgtccgc gggcgcggga tcgtcaccga tcccgtgttc gggcgccgct actcgccgct
     1801 caacggccgg cggatcccct ccccgccgcc gagcgcctac gaagagaccc gcgtgatcct
     1861 gatctcgcac gcccactacg atcatctgca gccccgcacg ctggcccgct tccccaggcg
     1921 cgcgaccatc ctctgcccgc cgccttcgga gcggtacgtg cgaaacctgg ggccgcgcgt
     1981 gcgcgtgatg cgggtcggcg aggagtacac gatccggggt gcctcgatcg tggcggtccc
     2041 tgccgatcat ccgggcggcc gctattcgca caccgccgag gcggacgggc gcgccctggg
     2101 gtacgtcatc cggaccgaga tcggcacgct ctactacagc ggggacaccg attacttccc
     2161 cggcatcgag acgatcggcc ggagctacaa gcccgatctc gcgctcctca acgtaaacgc
     2221 ccatcttcgc cccgccgacg cgctgcgcgc ggccgaagcc ctgggatcgc cccccgtcgt
     2281 cccgctccac ctgggcgcgt acggaggaag ggcggcgcgg cgcggccatc gctggcatcg
     2341 ggagttcctc gagatggcgg gcccgctggc gctgccgctc gcggtcggac agagcgtcat
     2401 cctgaacgga tcggtctccg gtccggtggt cgcgagccgc cccgcgctga ggctggtccg
     2461 ggacttcacg gcgtaaccag cgaaacgaag cggggagccc gatggggctc cccgcaggca
     2521 tgactccctc tcgacgagac gactacttcg tgactttggc ccctaccccc atggcggcgc
     2581 tcgacgcgac acccgcgcgg gtctgacccg cggcggcatg cgtgctccct gtggaggcca
     2641 cgcgcgtgtt cgctccgatc ttcgccggct tgccgtccgc cttcatggtc cccctcgcga
     2701 ccaaggtctc ggtcttgacg gcggccgcca cgcttccaac ccgaagcccc atgccgtacg
     2761 cgacctgtcc ccagcccatc ccgtccgcgt ggagcgcgta cagctggtcg atcgtcgttc
     2821 cgctcggcgc gttcgcgagg agcgtgtgcg cgatcatcac ttcaccccat cccacgttgt
     2881 actgggtccg ctcggcgagc agcgcttcgg aggtcacccc gaattccgcc gcgaaacggt
     2941 ccgctaccgt ggcgtcgccg ttcgcgacgg cttcgacgtc cacgtccttg atgttgccct
     3001 ccagcttctt ctgaatctcc tcgcgatcgg tcgccggcat cttcgcggcc cggtcgcgga
     3061 tcgccttgag ccgcgcctcg ttcttggcgg cgtcgggcgc cggcgcggtc gccgtcgtgg
     3121 tcgcctcccc gctcgtcgtg gtctgggttg cgggaacagg ctccggtacg gacgtcgcgc
     3181 ctaacgggac cacccctccc tgtttcgcgt cgttcccttc cgccgcggcc gccgccgtga
     3241 aggccagcac cagtgccgcg gtgagcgacg gaacgatgat tcgcctcatc cattcctccc
     3301 ttcgtgtgcg ccttgcggcg ccgtctcttc cgggcgctcc gcctcgcgga tcacggaatc
     3361 aggaatcgaa cgcccagaag tccaaccgtc tcgacactgg tatcggtcac ggtgcgctcg
     3421 cccgccgaac ccgcgcccag cccgcccgcg agtgacagcc ggtgcagcgg cccgcctccc
     3481 gacgtggagg tcgtggaggt ggccacgcct ctgccgacgt cgagctcgcc aaggatctgc
     3541 acgtgggaga aggcgcccca gacgaacgat ccgctcgcct gggacgcgtt ggcgtcgccc
     3601 tcgcgcttgc cggccatgcc gccggcccgg agcgcgaggc ccggaacgac cgacgtgccg
     3661 acctcgacgc cgatcgcgtt caatctcgcc gacacgttgt cttcctggtg gagtccgtag
     3721 acgcccgccg tgacgaggcc gagcgaccac tcggggccgg cgcgcagctt ccacgcccgg
     3781 tagccgccgt cgccgaagga gcgggcgccg atcgcgcgga tccgcgcgag ccccggcacc
     3841 ccgacgccgg cgttcgcgaa gagcgcgttc ccgtagccgg tcacggaatc gtcgtagcgg
     3901 atgagggcca gggtggcgtc agccagcggc acggacaccg tgccgaagag aaggcccgcg
     3961 gtgtaggtct ggtggcccgg tcccgcctgc cggtccacgc cggcgccagc gctcaagggc
     4021 gaggcacagg cggagcccaa gcgtccgccc gtgaggagca cggcgcaaag caggagcagc
     4081 gtggagccgg ttggcggagc aggtcgtcgc atcgtgggct cgctgggaac gcgctcgccg
     4141 ctgtccccgg gcccgacacc cgggacgtgc catggcgagg cggcaccttg cctttatcgg
     4201 cggaatcggg ggcgttcttg agcgaaaagt gcggtgggac ctacggctcg acggtgtaga
     4261 ggatgtcgac ccggttctgg cgggcggtct cggcttcgat cgtgaagatg cggttcagga
     4321 tgtagcgcag gcggagcgag gaggtggact ggaacacgtc catgccggca ctgacgtaga
     4381 gcttgggcga gagatgggtg cccagggata gcgacgtgct ctggagcgag gtgccgacgt
     4441 cgaggcgcgc ctgctcgatc cccagctccg aggcgactcc ctgcgcgagc aggttggtcc
     4501 ccggcacccc catggtcgag gcgagggtgc tcgccatctg cccctccgag aggttggccg
     4561 actcgatcgg ctttccgaac atgatgtagg agagcgcctc gctctgcccc atcgccggct
     4621 cggagaagac ctgcacgtcg ggcttcgaga ccgtgccgct cacgatgaat cccgcgacgg
     4681 ttccgtcggc ggcggtgcgc gtggcgcgcg cccgcacggc ggggtcggtg atcggccccc
     4741 cggcgaagat gagcgtcccg gtctcgacgg cgaggtcctg gccgtagatg tggtaggtgc
     4801 cgtccttgat gtccaggcgg ccgttcccca gcgtcggcaa tccgggcgtg tcgatcgcga
     4861 cgacgctgcc ggtcggccgc acgtcgaggc cgaatccggt cacgcgcact ttgtcgccca
     4921 tgaccagccg gacgcgcgtg tgcacctcgg tgggcatctc cgagagcgtg tcggcgcccg
     4981 tgaagaccac gtcgggcgac gtcttgacca ggcggcgctc gtcggcccgg ccgatcgtga
     5041 tggttccctc gggcacgtcg acctcgcccg tgatgtcgag gcggtttccg ttcagggcga
     5101 acttgaggtc gggcgaggcc acgacctgca tgtcgcgcgt gttcatcgcc tgcacgcgat
     5161 tccccttcac cgtgaccgtc gccacgggac gcgcgccgcg cgccaccgcg gcggtgccgt
     5221 cgatggccag cgtgcccgag cccgaccgca cggagccgtg cagctcgatg gcgccgccga
     5281 ggctgccccg tccctcgacg ttcatgtcgc ggagctggag cccgtagcgg ggaatgttcg
     5341 cgctgccgtt tcgaagcgtg agcggaccgt acaggaaggg gcgctgcacc gtgcccgcga
     5401 tcgcgaggtc ggcgtcgagc gctcccgagg tggcgtcgac ctccggagtc agcccctgcg
     5461 ccagggacag gtcgcgaaga tgcagggcga ggcggccgtc gatggggcgc gacgtgcctt
     5521 ccgcgaagga gccgtacgcc ggccagccca ggttcccccg gaccgtcccg acgttgacga
     5581 gatcggcggc gagcgtggtc tgcacgcgcc ggccgtccgc gacggccgtg aggcgcgcgt
     5641 tctggaaccg ggtcgggacc cagaggccct ccgcggtctc gtggaggatc tcgccgggac
     5701 ccggaacgac gtccacgtcc gcgaagagct gcccctcggc ggtcccgtgc gcgctgacat
     5761 ggccggccat cgtgccgctc aggcgtagcc gcggcggcag cccgggctgg agcagcgtga
     5821 ggttcacctg ttccagccgc gcgtcggcgt gccaggggcc gcggcgggcc cagtccgcgg
     5881 tggcgacgat gcgcgacgtc cccgactgcc agttgaattc ggtgaccgcg acccgcccgt
     5941 tcacggcggt gaggcgcgcc ggcgcggcca gcgaccagtt tcccgagcgg gggttcacca
     6001 ggtccagccg cgcgatcgtg ccgcgccagc ctcccgccgc gaagccgccc gcgagcacgg
     6061 cgacggtgct gtcggccctc ccggccaccg aagcgcgcca ctcgtgccgc tcgcgcgtgc
     6121 cgcgcgcggt gacgatcatg cggtcggcgg cgtggatccc cgcgctcacg cggctcgccg
     6181 agaggtccag gcggacgatg ccacccgggc gaaggtccac gtcgccgtcg gcgcgcagcg
     6241 cgccggcgtg ctcgttggcg aagaagaggg agtcgccggt gacggtcccc gcgatgtgcg
     6301 ggtgcgccct cgtgccgcgg atcgtcccct gcgcctcgag cgatccgctc gcctgcgcca
     6361 ggacggcgcc gagcttgggc gcgctcaccc tccaggcgag gtcgaaggaa cggccgaagg
     6421 cgccgctcgc ctggaccgag tccggccccc aggtggccgt cgcgcgatcg aggcgatact
     6481 ggccgcggtt ggcgacgagg gtgccggccg cggtgaccgg ctggttgcga acggtgccgt
     6541 cgaggcggct cacgagaatc cggccgctcg gtcccgaggg cccgttcacc ccctcgctac
     6601 gcccggcgaa gtccaggcgg ccgggatacg cccgccagac cgtgccggga tcgatgccgc
     6661 gtccgtcgaa cgacagccgc cagctcacgc ccggctgcca ggccaccgag ccggacccgg
     6721 tgatccttcc gccgaagatg ttcgcgatca cggtgtgcac cgcgagccgt ccgttcccgc
     6781 cgcgcccgtc cagcgtccag tgtccgggcg ggacgtttcg ccccgcgagg agcgcctcgg
     6841 aatggatgtc gaaatcggcc atcgtgcccc gcacgcgggc cgagccgcgc tcgctctcca
     6901 cccaccgctt cccgcgcagc ggccagccca tcgccgccca ctcggcgcgg agatcgaacc
     6961 gcgggttggc ggaatcgccg gacaacgtcc cccgcgccgt cacgcgtccg ggagcgcccg
     7021 gctgggtcac ggcaaggtcg tcgatctgcc agtcgccgcg ctcgaggcgg cgcacgcgga
     7081 agtccgcggc ggtgggaccg agcgcttccg tcgtcccccg gatgcgcccc tgcgcggtgg
     7141 cggcgcgcgc gtcgccgtcg agggcgacgt gcccctcgaa gattccctgc ggccatgcgg
     7201 gcccgaaatc ccgtggggta agacgagaga actccacgtc acccctaaaa cgggcctgcc
     7261 ggcgcagcgg gcggatcagc gtgacgtcca cctgcgcatc gaagggaccc ttcaggctct
     7321 ggcggacgcg cagggcctcc agcgttccgg agagggtgcc gtcgccctga atgggagcgc
     7381 tgccggcggg ccggtaggtc cacttcccgc ggagcgccag cgggtaggcg ccgcgcggga
     7441 gcgccgatcc ggagaaatcg aggtcgatcg ttcgggagcg gaccatgagc gcgttcacgc
     7501 gaacgctgtc ccggcgcatg cttcgcgcgt ccagccgcac ttggtcgatc acgaggcccg
     7561 aatcgctccc gggccgcgcg agggtcagcc cgttcacgac gccgttccgc acgatgacgg
     7621 tcacgggcag gttgagatcc ggcagcccgc gcagcgaatc ctgcgcctcg ggcgtgtccc
     7681 ccggacctcc cacgacgatc cgcgcgttct tggcgtagag attgtggatg tccagccggc
     7741 gcccccacag ctcgcgcagg cgccagtcga tgaagacgct gtccgcggtg atggccagcc
     7801 ggtcgttgcg gtaggtgaag tggcggacga tcaggggacc gcgcagcggg ccggtcatgt
     7861 cgcggacgtc gaggcgcccc gggaagaagc tccccagccg ctcgaacccc cacttggcac
     7921 ccggctgcgt gaagaagagc caccagctcc ccgcgaggac gaccgcgacg agcgagaccg
     7981 cggacagccc cgcgacgagg tatcgacgcc ggcgcggggt cacagctccg ctcccattgc
     8041 caggtgcacc tggatcggcg tgccccgccg gtccaggccc cacgcgacgt cggcgcgaac
     8101 gagcccggcg ggcgagatcc agcggagccc gaatcccgcg ccgcgcttca ccggaagacg
     8161 ccaggagttc atggcgttgc ccgcgtcgaa gaagaccgcg ccgccccacc gtggaaggaa
     8221 gaactgattc agctcgaccg ttccggtcag gaggtagcgg ccgccgatca cgtggcgcag
     8281 ctcgtcgcgc gggccgaggc tgttatagct gtagccgcgc acgctgttcg tgccgcccgc
     8341 gaagaagcga agcgaagggg gcaggctgcg gaagtcggcc gtgtgggtgg cgccggcctc
     8401 gatgcgggcg atgccgcgcg ttctggggcc gaaggcgtgg atcagcttgg cgtcgccgcg
     8461 cacctgttgg aacgagacgt ccgagaccac gcggcggtcg ccaaggcgag cgttcaggaa
     8521 gagccggtcg ccgttcttgg tgaagagcgc gtcgtcggcg cgaacgcgcg tccaggtacc
     8581 ctcggggacc agcgtcttca cgatgccgct gtccacgccg acctggaaat tctcgcgccg
     8641 gtagttgagc gcggggatca cgcgccactt cccgagcagg cggctctcgc tgatcccgct
     8701 gcggaagacc ttgctgtgga acgtggcggt ggccagctcc tggtagcccg tggagagggt
     8761 gagcaggtcg gttcgggcgt ggggccaggg gatgaagtat tccccgatgg cgtgccggtc
     8821 gcgcgatccg tactggagat tgatctgcgc gcggtgtccg gcgcgattca gccggcgcat
     8881 ctcggtcacg agccgaacgc gcgccccgtc gtcg
//
LOCUS       DASSLZ010000012         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1526805, whole genome shotgun sequence.
ACCESSION   DASSLZ010000012 DASSLZ010000000
VERSION     DASSLZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526805"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VFM00_00420"
     CDS             complement(<1..871)
                     /locus_tag="VFM00_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496741.1"
                     /translation="MRRTFERFARFAFALLLGAGPAAASPRTARETIAYVPAAIWGDY
                     LEPATAGLALTPHAIYWFEGVDSSMGRILGDLERYQADDAKAAPREPMVAFVGRYAAY
                     PSNTLVPGVAVMDTAGALIADFPRGRSYAWSPDGTRLVVLFPREALPRGRGARAASAA
                     GRAASAAGRLRAAVRYRPGVSVWDRRDGSVSSFARWPSRAAWAGNDSLLLQFRDSVLV
                     LDARTGKTARTGHNGTIVSTDSRYALWPGEDGQNTRVFEEESGERITDAVFGPFQQSG
                     KGQIRSAFWVRGRG"
     gene            complement(1040..2395)
                     /gene="lat"
                     /locus_tag="VFM00_00425"
     CDS             complement(1040..2395)
                     /gene="lat"
                     /locus_tag="VFM00_00425"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HET7496742.1"
                     /translation="MTPTATGTSVRVTPDQVHATLAKHMLADGFDFVLDLDKSQGVWL
                     HDSRGNKTLMDFFSFFASNPVGMNHPGIKNPEFQARLVKAALHNVTNSDVYTTEMAEF
                     VETFARHAMKDAFVHSFFVAGGTLGIENALKAAFDWKVRQNFARGWREERGAKVLHFE
                     QAFHGRSGYTLSLTNTTDPRKTAYFPKFEWPRVSNPKAEFPLTEDRLKDVKAREALAV
                     AQMDRAFERYGDDIAAIIIEPIQGEGGDNHFRAEFFKELRRFADEKGAMLIFDEVQAG
                     FGLTGKFWAYEHYGVAPDMVCFGKKAQICGFIANKRIDEVKDNVFHVSSRINSTWGGT
                     LVDMVRSQRYLEIMAEDRLLDNAAARGAELLAGLEGFAKEFPEHVMNARGKGLMCAFD
                     LRDGGLRDAVIANAYAEGVVVLGCGPTSIRFRPPLVISKEEVAQGLEKFRSSIKKAIG
                     R"
     gene            complement(2466..>2710)
                     /locus_tag="VFM00_00430"
     CDS             complement(2466..>2710)
                     /locus_tag="VFM00_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HET7496743.1"
                     /translation="FVEHPKFETPFIEAVAELDEVLECHHITGEFSLLLKVKVTDMAA
                     FRRLLIEKLNTIRGVRQTRTVMVLSTWKEQHRIKVD"
BASE COUNT          414 a          915 c          934 g          447 t
ORIGIN      
        1 cgccgcggcc gcgcacccag aaggccgagc ggatctgccc cttccccgac tgctggaacg
       61 ggccgaacac cgcgtcggtg atccgctctc cgctctcttc ctcgaaaacc cgcgtgttct
      121 gcccgtcctc tccaggccat agcgcgtagc gcgaatcggt cgagacgatg gttccgttgt
      181 ggccggtgcg agcggtcttc cccgtgcgcg catcgagcac caggacgctg tcccggaact
      241 ggagcagaag cgagtcgttt ccggcccacg ccgcgcgcga gggccagcgc gcgaaggagc
      301 tcaccgagcc gtcgcgacgg tcccacacgg aaacccccgg ccggtatcgg accgcggccc
      361 tcaagcgacc ggcagcgctc gcggcacggc cggcggcgct cgcggccctc gctccgcggc
      421 cgcgcggcag ggcctcgcgc gggaaaagga ccacgagacg ggtgccatcg gggctccatg
      481 cgtagctccg gccgcgcggg aagtcggcga tcagggcacc ggccgtgtcc atgaccgcca
      541 cgccgggaac gagcgtgttc gaggggtagg cggcgtagcg gccgacgaaa gcgaccatcg
      601 gctcccgcgg cgccgctttg gcgtcatcgg cctggtagcg ctcgaggtcg cccaggatgc
      661 gccccatcga cgagtcgact ccctcgaacc agtagatcgc gtgcggcgtc agcgcgagtc
      721 ccgcggtggc cggctcgagg tagtcgcccc agatggcggc ggggacgtag gcgatcgtct
      781 cgcgcgcggt gcgcggggag gcggcggcgg gccctgcccc aaggaggagc gcgaaagcga
      841 agcgagcgaa ccgttcgaag gtgcggcgca tgggtgtctc ggtcctggtg gcccggcgga
      901 gcgttttcgg cggagcttcc gtgctgcctg cggaaattat agcaggaaac gaaagagggg
      961 cgccgcttgc gcgacgcccc cctgattcac ggccgccggc gtcttgcgtc gccggcgcgc
     1021 cgctacctgc cccgctcgct taccgcccga tcgccttctt gatgctcgac cggaacttct
     1081 ccagaccctg cgcgacctct tccttcgaga tcacgagcgg cggacggaac cggatcgagg
     1141 tcgggccgca gcccaggacc accacgccct cggcgtaggc gttcgcgatc accgcgtcgc
     1201 gaaggccgcc gtcccggagg tcgaacgcgc acatgaggcc cttgccgcgg gcgttcatca
     1261 cgtgctcggg gaactccttg gcgaagccct cgaggccggc gagaagctcg gcgccgcgcg
     1321 cggccgcgtt atccaggagc cggtcctcgg ccatgatctc gaggtagcgc tggctgcgca
     1381 ccatgtccac cagcgtgccg ccccaggtcg agttgatgcg gctggagacg tggaagacgt
     1441 tgtccttcac ctcgtcgatc cgcttgttcg cgatgaagcc gcagatctgg gccttcttgc
     1501 cgaagcagac catgtccggc gccacgccgt agtgctcgta cgcccagaac ttgccggtga
     1561 gcccgaagcc cgcctgcact tcgtcgaaga tgagcatggc gcccttctcg tcggcgaagc
     1621 ggcgcagctc cttgaagaac tcggcgcgga agtggttgtc gccgccctcg ccctggatcg
     1681 gctcgatgat gatcgcggcg atgtcgtcgc cgtagcgctc gaacgcacgg tccatctggg
     1741 cgaccgcgag cgcctcgcgc gccttcacgt ccttcaaccg gtcctcggtg agcgggaact
     1801 cggccttggg gttggacacg cgcggccact cgaacttcgg gaagtaggcg gtcttccgcg
     1861 gatcggtggt gttggtcagc gacagggtgt agccggagcg gccgtggaag gcctgctcga
     1921 aatggagcac cttggcgccc cgctcctcgc gccagccgcg cgcgaagttc tggcgcacct
     1981 tccagtcgaa ggccgccttg agcgcgttct cgatgcccag cgtgccgccg gccacgaaga
     2041 aggagtgcac gaaggcgtcc ttcatggcgt ggcgcgcgaa cgtctccacg aactccgcca
     2101 tctccgtggt gtagacgtcg ctgttggtga cgttgtggag ggcggccttc accagccgcg
     2161 cctggaactc cggattcttg atgccggggt ggttcatccc gaccgggttc gaggcgaaga
     2221 acgagaagaa gtccatcagg gtcttgttgc cgcgcgaatc gtgcagccag accccctggc
     2281 tcttgtcgag gtcgaggacg aaatcgaagc cgtcggccag catgtgcttg gccagcgtgg
     2341 catggacctg atccggcgtg acgcggaccg aggtgccggt ggcggtcggg gtcatggcgt
     2401 gctcctttcg ggtgttcgga cggagaatcg tttcgcttcg cacaatcgat cggccgcggg
     2461 ccgcatcaat ccactttgat ccggtgctgc tccttccacg tggacagcac catcacggtc
     2521 cgggtctggc gcacgccgcg gatcgtgttc agcttctcga tcaacaggcg gcggaacgcc
     2581 gccatgtcgg tcaccttcac cttgaggagc agcgagaact ccccggtgat atggtggcat
     2641 tccaggactt cgtccagttc cgcgaccgcc tcgatgaacg gggtctcgaa cttggggtgc
     2701 tcgacgaaga
//
LOCUS       DASSLZ010000013         4625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_6726, whole genome shotgun sequence.
ACCESSION   DASSLZ010000013 DASSLZ010000000
VERSION     DASSLZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6726"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VFM00_00435"
     CDS             <1..713
                     /locus_tag="VFM00_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HET7496744.1"
                     /translation="SLLVLTDGERWMLFPAAKDHKRLEDGDRGPNTGGMGACAPARVP
                     PAEEAIAIGRRIVDPVLRALREEGTPYRGILYVGLMLTREGPSVLEFNARFGDPEAQV
                     VLPLLAEDPFPLFRAAARGALPAERHAAFVAHEGAAVCVVLASRGYPERSESGVPIEG
                     LEGPWPHGLSIFHAGIDRRGDRWVTAGGRVVGITARAETLERAREAAYGAVARIGFPG
                     MRYRKDIAAKPLEAGAAS"
     gene            710..1183
                     /gene="purE"
                     /locus_tag="VFM00_00440"
     CDS             710..1183
                     /gene="purE"
                     /locus_tag="VFM00_00440"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET7496745.1"
                     /translation="MSAPKVMILIGSESDRATLEEAVKVLDRLGIPNELEQASAHRSP
                     DRVRELVRGGPSRGVHVFIAAAGMANHLAGAVAAHTTRPVIGVPLGGSPLGGADALLS
                     TVQMPAGVPVATVAIGSAGAKNAAVLAAQILALADPALQIKLDDLKERLARGEKL"
     gene            1200..1844
                     /locus_tag="VFM00_00445"
     CDS             1200..1844
                     /locus_tag="VFM00_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HET7496746.1"
                     /translation="MAERIVLGGRSDATRDEAVRRAGAVLREGGVAILPAEGLYGYHA
                     LAASAPGIARLESLKPRGPGKGWIGLVARPGDAYRWIAALPAPAAALIHAHWPGALTL
                     VLDAGPSVPPSLLGPGETVALRCPGSSFLREVILAAGGLVVSTSANAPGAPPAVSAPP
                     PDAVPDGVSLVVDSGPLSGAPSTVVRAEGERVTVLREGAVILPEAPLDAPPGGP"
     gene            1886..2371
                     /locus_tag="VFM00_00450"
     CDS             1886..2371
                     /locus_tag="VFM00_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HET7496747.1"
                     /translation="MFQVLIVCTGNTCRSPMGEAILRSILPADIAERVTVSSAGSSAG
                     EGAPAAAYAIQTASSRGLDLTKHRSRPLTAALIREADLVLGMEPAHVSAAKSLAPDSA
                     DRVHLVSEHGAEGGASDGVRDPIYGGPDDYVDTFNRIRSHMLRWLPVIREAVERREGV
                     R"
     gene            2414..3376
                     /locus_tag="VFM00_00455"
     CDS             2414..3376
                     /locus_tag="VFM00_00455"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET7496748.1"
                     /translation="MSREPQGFIWMNGKFVAYEDAKIHVLSHVVHYGSSTFEGIRAYD
                     TPKGTAVFRLDRHMQRLLDSCKISRIDCPYSVGELMDAAKETVKKNGGGACYIRPVVY
                     RGHKTLGVNPTGVPVDVAIATLNWGKYLGAEALEAGIAVKVSSWRRAAPDTFPFMAKT
                     GANYMNGQLIKLEAVTDGYEEGIALDSFGFVAEGSGENIFLVKNGVLSTPTIASAILP
                     GITRDSILEIAKDLGIETREEQIPRESLYIADEVFFTGTAAEITPITSIDRLPVGNGR
                     VGSMTRRLQESFFDIIEGRERDRHGWLFPVEARAKVQRAAKKAR"
     gene            3404..>4625
                     /gene="glyA"
                     /locus_tag="VFM00_00460"
     CDS             3404..>4625
                     /gene="glyA"
                     /locus_tag="VFM00_00460"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7496749.1"
                     /translation="MNPRTPDAPVSDAPAALPAAARPLAAVDPEIDAAIVAEVRRQQS
                     TVELIASENFVSEAVLEAMGSPLTNKYAEGYPGKRYYGGCEFVDVAETLAIERAKALF
                     GAEHVNVQPHAGAQANFATYMALMAPGSTLLGMALAHGGHLTHGHKVSYSGIVFKAAQ
                     YGLSPETGLLDYDQAARLAREHRPAVLVAGASAYPRFFDFARLRAIADEVGARFVFDM
                     AHVAGLVAAGVHPSPVPFADAVTTTTHKTLRGPRSGMILCRSEFAKAIDKSVFPGMQG
                     GPLMHVIAAKAVCLKEAAAPEFRTYAERVVENAKTLAAELTARGFQLVTGGTDNHLLL
                     VDLKQAGFSGADAETALHAAGITVNKNAVPNDPRPPAVTSGIRIGTPAVTTRGFGTAE
                     MRRLAAWIDEAIQKR"
BASE COUNT          678 a         1656 c         1621 g          670 t
ORIGIN      
        1 cctcgctcct cgtgctcacc gacggcgagc gctggatgct ctttccggcc gcgaaggacc
       61 acaagcgcct ggaggacggc gatcgcggcc ccaataccgg cggcatgggc gcctgcgcgc
      121 ccgcgcgcgt cccgccggcc gaggaggcga tcgcgatcgg ccgccgcatc gtcgatcccg
      181 tgctccgcgc gctgcgcgag gagggaacgc cgtaccgcgg cattctgtac gtcggattga
      241 tgctcacgag agagggccct tccgtgctcg aattcaacgc gcgcttcggc gatcccgaag
      301 cacaggtcgt ccttccgctg cttgccgaag atccctttcc gctcttccgc gccgccgcgc
      361 gcggcgcgct ccccgccgag cggcacgccg cgttcgtcgc gcacgagggc gcggcggtct
      421 gcgtcgtgct cgcctcgcgc ggctatcccg agcgctcgga gtcgggcgtc ccgatcgagg
      481 gcctcgaggg cccctggccg cacggcctct cgatcttcca cgcgggcatc gaccggcgcg
      541 gcgaccgctg ggtcacggcc ggcgggcggg ttgtcggaat caccgcgcgc gccgagacgc
      601 tggagcgcgc gcgcgaagcc gcctacggcg ccgtggcgcg gatcggattc ccggggatgc
      661 gttaccggaa ggacatcgcg gcgaagccgc tcgaagcggg ggcggcgtcg tgagcgcgcc
      721 caaggtgatg atcctgatcg gaagcgagtc ggaccgcgcc acgctggaag aggcggtcaa
      781 ggtgctcgac cggctcggca tccccaacga gctggagcag gcttcggcgc atcgcagccc
      841 cgaccgcgtg cgcgagctgg tgcgcggggg cccatcccgc ggcgtgcacg tcttcatcgc
      901 ggcggcgggg atggcgaacc acctcgccgg cgcggtcgcg gcgcacacca cgcgccccgt
      961 gatcggcgtg ccgctgggcg gctcgcccct ggggggcgcg gacgcgctcc tctccaccgt
     1021 ccagatgccg gcgggagttc cggtcgcgac cgtggcgatc ggctcggccg gtgcgaagaa
     1081 cgcggcggtt ctcgcggcgc agatcctggc gctggccgat cccgcgctcc agatcaagct
     1141 ggacgacctg aaagagcggc tcgcccgggg cgagaagctg tagcccaggg cccggcgcgg
     1201 tggccgagcg gatcgtcctg ggcggacgga gcgacgcgac gcgggacgaa gcggtccggc
     1261 gcgccggagc ggtgctccgc gagggaggcg tggcgatcct gcccgccgag ggtctctacg
     1321 gctatcacgc gctcgccgcc agcgcgcccg gcatcgcgcg gctcgaatcg ctgaagccgc
     1381 gcggcccggg gaagggctgg atcgggctcg ttgcgcgccc cggcgacgcc tatcgctgga
     1441 tcgcggcact tcccgcgccc gctgccgcgc tgatccacgc gcactggccc ggcgccctca
     1501 ccctcgtgct ggatgccggc ccttccgttc caccatcgct cctgggcccc ggcgaaacgg
     1561 tcgccctccg ctgcccagga agctccttcc tgcgggaggt gatcctggcg gccggggggc
     1621 tggtcgtgtc cacttcggcg aacgctccgg gagcgccgcc ggcggtctcc gcgccccctc
     1681 ccgacgccgt gccggacggg gtttcgctgg tggtcgattc gggccctctt tcgggcgctc
     1741 cgtccaccgt ggtacgcgcg gagggagagc gggttacggt gctccgcgaa ggggcggtga
     1801 tcctgccgga agcgcccctt gacgcccccc cggggggccc ctagactggg cttcgcgacg
     1861 ctgtttctcc aacattgcga ggctcatgtt ccaggtcctc atcgtctgca ccgggaacac
     1921 ctgccgtagc ccgatgggcg aagcgattct tcgctccatc cttccggccg acatcgccga
     1981 acgcgtgacc gtatcgtcgg cggggtcgag cgcgggggag ggagcaccgg ccgccgccta
     2041 cgccatccag accgcctctt cacgtggcct cgatctcacg aagcaccgct cccgcccgct
     2101 cacggcggcg ctcatccgcg aagccgatct ggttctcggg atggagccgg cccacgtctc
     2161 cgccgcgaag agcctggcgc ccgattctgc ggatcgggtc cacctggttt ccgagcacgg
     2221 ggccgagggg ggagcgtcag acggcgtcag ggatccaatt tatgggggtc cagacgatta
     2281 cgtcgataca ttcaaccgga ttcgaagtca catgcttcgc tggctcccgg tcatccggga
     2341 ggctgtcgag cgacgggaag gcgttcgata ggcggcgtcc gtccggacgc cgtggccgtt
     2401 taaaggagga gcgatgtctc gcgagccgca aggcttcatc tggatgaacg gaaagttcgt
     2461 cgcgtacgag gacgcgaaga tccacgtcct ctcgcacgtc gtgcactacg gttcgagcac
     2521 gttcgaaggg atccgcgcct acgacacgcc gaaggggacg gcggtcttcc ggctggaccg
     2581 gcacatgcag cgcctgctcg actcctgcaa gatctcgcgg atcgactgcc cgtactccgt
     2641 cggcgagctg atggacgccg ccaaggagac cgtcaagaag aacggcggcg gcgcctgcta
     2701 catccgtccc gtggtctatc gcggccacaa gacgctcggc gtgaacccga ccggcgttcc
     2761 ggtggatgtg gcgatcgcga cgttgaactg ggggaagtac ctcggcgccg aggcgctcga
     2821 ggccggcatc gcggtgaagg tctcgtcgtg gcggcgcgcc gcgcccgaca cgttcccgtt
     2881 catggccaag acgggcgcga actacatgaa cggccagctc atcaagctcg aggcggtcac
     2941 ggacggctac gaagagggga tcgccctcga ttccttcggc ttcgtcgccg aggggagcgg
     3001 ggagaacatc ttcctcgtca agaacggcgt cctgagcacg cccaccatcg cgagcgccat
     3061 cctgccgggc atcacgcgcg acagtatctt ggagatcgcc aaggacctcg ggatcgaaac
     3121 gcgcgaggag cagatcccgc gcgagtcgct ctacatcgcc gacgaggtct tcttcaccgg
     3181 gaccgccgcc gagatcacgc cgatcacgtc gatcgaccgg ctcccggtcg gaaacggccg
     3241 cgtgggatcg atgacgcgcc ggcttcaaga atccttcttc gacatcatcg aggggcgcga
     3301 gcgcgaccgg cacggctggc tcttccccgt ggaagcgcgc gccaaggtgc agcgcgccgc
     3361 gaagaaggcg cgctagcgct ccgcgccggc cccgcgatcg gtcatgaatc cgcgcacccc
     3421 tgacgctccc gtttcggacg cgcccgccgc ccttccggcg gcggcgcgcc cgctcgccgc
     3481 ggtcgatccc gagatcgacg ccgcgatcgt agccgaggtc cggcgccagc agagcaccgt
     3541 cgagctgatc gcttccgaga acttcgtgag cgaggcggtg ctcgaggcga tgggatcgcc
     3601 cctcacgaac aagtacgccg agggctaccc cggcaagcgc tactacggcg gctgcgaatt
     3661 cgtggacgtg gccgagacgc tcgccatcga gcgggccaag gcactcttcg gcgccgagca
     3721 cgtgaacgtg cagccgcacg cgggggcgca ggccaatttc gccacctaca tggcgctgat
     3781 ggcgccgggc tccacgctgc tgggcatggc gctcgcgcac ggcggccatc tcacccacgg
     3841 gcacaaggtc agctactccg gcatcgtctt caaggccgcg cagtacggcc tgtcgcccga
     3901 gaccggtctc ctcgactacg accaggcggc gcggctcgcc cgcgagcacc gtccggcggt
     3961 gctggtcgcg ggggcgagcg cctatccgcg cttcttcgac ttcgcgcggc tccgggccat
     4021 cgccgacgag gtcggcgcgc gcttcgtctt cgacatggcg cacgtcgcgg ggctggtcgc
     4081 ggcgggcgtg catcccagtc ccgtcccgtt cgccgacgcg gtcaccacga cgacgcacaa
     4141 gacgctgcgg ggaccccgca gcgggatgat cctctgccgc tcggagttcg ccaaggcgat
     4201 cgacaagtcg gtcttcccag ggatgcaggg gggaccgctc atgcacgtga tcgcggccaa
     4261 ggcggtctgc ctcaaggagg cggccgcgcc ggaattccgc acgtacgccg agcgcgtggt
     4321 ggagaacgcg aagacgctgg cggccgagct cacggcccgc ggcttccagc tggtgacggg
     4381 gggaaccgac aaccacctgc tcctcgtcga tctgaaacag gcgggcttct ccggcgccga
     4441 cgccgagacc gcgctccatg cggccggcat caccgtgaac aagaacgccg tcccgaacga
     4501 tccacgtccg ccggccgtca cgagcggcat ccggatcggc acccccgccg tcaccacgcg
     4561 cgggttcggc acggccgaga tgcgccgcct cgccgcctgg atcgacgagg ccatccagaa
     4621 gcgcg
//
LOCUS       DASSLZ010000014        15439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1020313, whole genome shotgun sequence.
ACCESSION   DASSLZ010000014 DASSLZ010000000
VERSION     DASSLZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 15439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15439
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020313"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..5097
                     /locus_tag="VFM00_00465"
     CDS             94..5097
                     /locus_tag="VFM00_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496750.1"
                     /translation="MDAAGLFTASAVGSGLVIATAGALSATAPVTVRPTRGIRIAGLT
                     APSALYQGQDSVLVRLTLTNLGADSIRLDPPALRFTRLIPGDADGDFDVSLAAGSPPG
                     IGPRGTVVVTALARVLTAASTGTVSIDASASAIEVVSDIAVQDPAADAPVVALITSGG
                     ISVTAAQVPSSVLPGARNVVLANLTVTNHYPDTRTVRSLRLTNRSSGLGDQDQLDDEL
                     GETALYKDDGDGALESGADTLLVATSALSGSVTFAPLSMALAPGSTARLLVTSSIPLD
                     ARDGDSLDVSLDDSTAVTFVRVPFFRTGWPLRPPGVFTVDGMAADQIRLMPGAGGNLL
                     AGSSNNPTLEFIVPPNGYRPDLLQKLAVVNFGTAEAANDITRLRAWVDDGDSTFDASR
                     DRLLGDLVNTGDRWQRTGLSETIPLGGLCLFVTVDLSDLARQGRTVQLGIPFGLDAGI
                     GVQSGNSGPRDRAVRSLAERAVSTVDRVTLTAADVESTVVHPGDRRVLLHHFVITNSY
                     ADPRTLTSVAFTNATLGPGGTAARDGEIESATLRLDGDGDGLLGDPAVDRPLGTSFFS
                     GGRAAFTGLSASIPAGGAVHLFLVADVARNGARDGDVLSAKVAGPLDVGFKEPTTTTA
                     AWPIDSRARATVDGLMAAQITNIGAAGVTIGPGDGPVLALNVVVPWNGYQTDTLRNVA
                     VVNLGSATPATGLQELRLWRDGGDGIFSAGGGDDVDLGVMLPDSSAQVWRSPFLSEAV
                     EGAGRRLFVSLGTSATARDSVTVRLAIPFGGIQMETGDDGPLDARVENEQAILISTSP
                     LLATLETASAASTVGQPITVRMIVRNVGAETVTGILPGPLRAGGTAGTVLDAAPGSPV
                     DLAPAAVDTFLWTYHSTSAGDVQFQGGAEGTGAETGLVRRAVETPSNVHRVYLAAAEL
                     ALFPVQSMPFHVTRGQTGVVPFSLTLTNGGGSGASDIRLRSLRLHLQDETGAGIVPAA
                     LLSHVSVNEGTNVYLSRSSLETTGSELNLTLASPLRITAQEPVTISIRLDIADSTTVP
                     AFRVLLADSTEILAEDATSGAPVTVRHEGGSFPVASGLARLAADATELDVASLGGPAS
                     RVAIGTTGVPLAAFRLENVGVAGITSDVQVNQIGVTVADTAGVPLAQAASYLSRIVVR
                     AGFQTFATQPVPAGTGPGVTLVLSPPLAVPAGTPVDLFLDGDVPDSARVGAFRLILQP
                     PSSFEAQDANSRDSVSVGYTGAPWGVAVLVEAPARSVAARGTALFPVSAVAGERDLAA
                     IRVVLRHPGSPGTGRIRADSLLVQVRDETRTARVPDLFLDRLRLFRDGVEVLNVTSFP
                     VAAVPVAVPLTGPLLEPGDSAAFTLVADLSPSAPASFLEVAIAGSGILASDANSGQPV
                     IAAPEPGVDFPLVSGLTHIASPARDLMARLVDRMPAALAVDGREVVTGVLTLRNAASA
                     GSGSIRVDRLRVRACDPGFRALAVGAAARRVAAYVNGALWAASDSLRADSTEATLSAA
                     APLDVDAGRTVSVELRLVPQPAPSAASFRVGIDAAGVGVVQPGSALLTVQVSPEAGTA
                     FPMWTESGSFTPASLSASWGNFPNPFAAGREATSFVYYLASSADVTLRIWTPSGEGVA
                     TLLDRAPRGAGLHQADAWNGRNGRGDVVRNGVYVAELIVKYADGSTERARRKVAVVR"
     gene            5094..7085
                     /locus_tag="VFM00_00470"
     CDS             5094..7085
                     /locus_tag="VFM00_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496751.1"
                     /translation="MRARAIAFLLAVATVGAAPRQARADGGDAGTRSPFAAPAGTRGI
                     ALGGAFVAVPGDVTIGAWNPGGLGWVARGEASASTVSYDLGFREDFATFAFPSWRLGT
                     ASVAFRRFGTDGIDGRDDRNVSTGEDFSSSETEVSVAYGRTFGTLVGVGGAVKMQRQS
                     VAGQSGSGLGADLGVTLYPLLALRVPVRWASQVTWGVGIRNLVEPSIRLDEESVRDPG
                     TRRMGFAYRTAFGLATLDVETGGGIRPRLHAGAEYAPRPYLSVRAGMNGGALTAGAGF
                     RARGVALDYAFEDRGYDPIHRVGLSYAFGPTTAERRDAALRAEDEKLQARLAETFQRV
                     QREKVRGLLTRAEEARAAGSYAEALEALATIGTLDPDNPEAADLEIRCLREQGATFEQ
                     EGNLAEAAMAYGRILEKRPQDPDARVAQGRCRAESDRRAARSAEIRKSFAQAMDAFAS
                     DNLVAARDGFAAILRETPNDDDARAMLRRTQTALSRRAQGILRDARRSLADRHLDAAA
                     TQLQEARGLDPGADGLASLAAALERARSTASARAPEPARSAPPPAPSAPTLRPGELRE
                     FYRRALSALAEKRSADALRYFELVWSADPKYQRVAEYLEREYLIQGLDAYAAGRLDDA
                     IAHWERALAVDPSDPRARGYLTRAQQQLERSREMAGDAP"
     gene            7082..9229
                     /locus_tag="VFM00_00475"
     CDS             7082..9229
                     /locus_tag="VFM00_00475"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7496752.1"
                     /translation="MIGRGERIIGIRWIVTFVAVGLTTAVVLSVGTVAERNARRALTA
                     ELTSRLLLEARNLALTSSGVLLSDFPELTLHPLIKEKLARQPELSYAVVVDRAGIIQG
                     HADPHRLGTPFEEPVGLRALMDGPPRSAGETLRRNDRELVASVPITHPSGKRLGHVVV
                     AMRTSYVDAVLERARRQQSLILAISLLIGIAVSFLVLSHVLRPVATLREGIRRIAGGD
                     LETPVRVSDGTELGLLANAINDMAAGLKKAQMEMVERERLVREMELAREIQDSILPSE
                     RIVEGPFVIEGSQRAAAEVGGDYYDYFLLPDGRIGFAVADVSGKGVAGCLIMTMLYAL
                     LRADRATASSPAALLASLDERLGETLRRGSFVTMFYGILDVRTGRLVYASAGHNPTLI
                     YRHASRSVETLRSRGIPLGAIRGGAIRGTLEDLTITLEPGDAVLQFTDGISEAFEASG
                     QEQFGHERMSATLRDTAPRGPRAVIDGFFERLTAWTGGGARLDDETILVLHRDPAHVP
                     APLESEPVGNGCGSPLPTREPDPGDSATAIARYDEARRRGSRVVLTGTSDVLDTIRGW
                     LAGTPVLDALPARERDLLTSTLYEVCANVAEHGYARDPGREYEVWWVPAPGPAEGARS
                     PRARAAAGTASERLRQGWFLVRDEGVPFSANEWKGQDFDDPRVRRRGRGLGLEIIHRT
                     MRRIVYYPSTAVGNVTWMCFGGSPSPEEASHAG"
     gene            9219..9566
                     /locus_tag="VFM00_00480"
     CDS             9219..9566
                     /locus_tag="VFM00_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HET7496753.1"
                     /translation="MPDSFGIQEETRGSAVLLRVRGRLDARSAPHLLEVGSRVRMRGR
                     NLVLDLSGVTFMASSGLGSLLSLAEEFRQTDFRLRIASPSAAVTSVIQLLNLQPFLSI
                     DASEEEALHALGV"
     gene            9550..10902
                     /locus_tag="VFM00_00485"
     CDS             9550..10902
                     /locus_tag="VFM00_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7496754.1"
                     /translation="MPSESDPVIPRPVEASTVPSGPGPSDEATRALRLVARREHELLA
                     LSELSQELTISLDLYGVADVVLFNLMGQLGTSRAALWLMSEHGRAVPVLLRSHGIDRR
                     TASALGSACSAWLLERFAQEPRIVLAAEMIEEPARAASLLARRESITLFAPILARGEV
                     LGFIALGPRVGPGTYGAVELQGLQTSLGMVGVALHNTRLYNRLLENNRRLRLANDNLM
                     ELDRLKSEFLSNVNHELRTPLTVVIASLQIVLEHDAPDPQLAEFLTAALGEAQSLKGS
                     LEKLLDLSDLRRNVVPFQFETGDVAAPLRGYYEERLPGVTQGLRELAFHAMPDLPPAR
                     FDRHRMLQVVDALVDNAVKFTPEGSRIDLAVRTAEREGRHWIRIDVADDGPGIATDHM
                     SVLFESFSQVDGSATRKVGGMGLGLAVARQLAERMDGALTAASKPGQGSVFSLYLPLE
                     "
     gene            10982..11404
                     /locus_tag="VFM00_00490"
     CDS             10982..11404
                     /locus_tag="VFM00_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET7496755.1"
                     /translation="MRHFRKTLTFEIPARMEFVNITPEVEAALRESGIAEGLCLVNAM
                     HITASVFINDDESGLHADYKRWLEELAPFDASPARYRHNRTGEDNADAHLKRQVMGRE
                     VVVAVTKGKLDFGPWEQIFYGEFDGRRAKRVLIKIIGE"
     gene            11497..13452
                     /locus_tag="VFM00_00495"
     CDS             11497..13452
                     /locus_tag="VFM00_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7496756.1"
                     /translation="MVASARQTARDYTVALSVTALAIIVRGAIDPLLGEYQPLALLYA
                     SVAITVWLAGLYPSVVACVAGYVASNILFIEPRGRFALPHAHDWLALGGYLLAASVLI
                     FVGESLRRNRAVLRERVDAGIRMERELSAARDQLLIVTDCMSALVLHCNRAFECTWVS
                     KTYASWLGLRPEEIVGRSIEDVIGREAFEQLRPHLERVLRGEIVRCEEEMPFPGQSSR
                     WISAVYTPTRDAHGEPEGWVGVVTDIDQRKREEQSLREADRRKNEFLALLAHELRAPL
                     APIRNAVRFLQIAAPQQGELQAARDIIDRQVDQMIRLIEDLLDISRITQGRIQIRKSR
                     MELGPVVQLAVEASRQLINLRDQSLTVTAPSEPVWLEADPARLAQILSNLLYNAAKYT
                     DPGGCIGLSVKREGHEVVLSVRDTGVGIRKEDLGAIFDMFAQRPLDPRNAVGGLGIGL
                     ALVRGLVELHEGSIEARSAGPGCGSEFIVRLPAPPSRGTHAAAEGEVAAGDGASLRIL
                     IVDDSRDTADSLRMLLSLGGHEVRTSHDGVQAVTEAEEFRPDLVLLDIGLPLMDGYEV
                     AREIRSRPWGPKTVLVALTGWSRDEDRIRTRQAGFDYHLTKPADPKELEALVGRLTGR
                     APTTGGLEAGEARGAEAPHPGTPMGAF"
     gene            complement(13449..14129)
                     /locus_tag="VFM00_00500"
     CDS             complement(13449..14129)
                     /locus_tag="VFM00_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET7496757.1"
                     /translation="MNPKGPGREDPDGRRRGAGPNAERSAAAKNRAVRIALAHDLVRA
                     GRILDRRGMLAASEGNLSARMTADRVLITRRGKRKGDLTPRDFVELTLMEPRDSAARG
                     AASTEHRVHLVAYAVRRDVEAIVHAHPVALTAFALRGHAPDFSRIDEARAYLGHVGFV
                     RYHASGSDDLAEAIGLALDPPGVVAPTVLVLQNHGAISVGTNVEEAVSRLEIAEHLAA
                     AMLLAERR"
     gene            complement(14164..>15439)
                     /locus_tag="VFM00_00505"
     CDS             complement(14164..>15439)
                     /locus_tag="VFM00_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET7496758.1"
                     /translation="ARIPITFLPDPRGWGPGSEVALAARGTAFLVDALLGTGSTGAPR
                     GAIGAAIEMADASGRPIASIDLPSGVDGSTGYAEEPAIRAAFTVTLAVMKTGLLLEPG
                     RTHAGRIEAVDIGIPPDLVAEQPASLLMADAGWARSLLPRRAADAHKGSMGRVLVVGG
                     SGGMMGAVAMASESVLRAGAGYAVAAVPRSCVDVLESRVPAVVKRGMPETPARALALD
                     ALDPILNEALHADVMLIGPGASRDPETADLVRALVERVECPILLDADGLNAFEGRPLR
                     RLHAPLLVSPHYGEAARLTGRLIAEVARDPMGWARRFSAQSNAVVCLKSTPMLTAAPA
                     EPLILNASGNPGMATAGAGDALSGVISGLLAQGMDPPEAAAVGCFVHGLAGDVAARRK
                     GMRGMIATDIVEAIPAALVALEIGAIEEPEAR"
BASE COUNT         2110 a         5638 c         5484 g         2207 t
ORIGIN      
        1 gagacccagt cgttccgcgc ggccgccttc gacgccttcg gacatccggt cgcgcgatcg
       61 ttcgcctggt ccacgcaagg agggatcggc acggtggacg ccgcgggcct cttcacggcg
      121 agcgccgtcg gctcggggct ggtgatcgcc accgcggggg cgctctcggc cacggccccg
      181 gtcacggtac ggccgacccg cggcatccgg atcgccggac tgaccgcccc ctcggccctc
      241 taccagggac aggactcggt ccttgtgcgg ctcacgctga cgaatctcgg cgccgactcg
      301 atccggctcg acccgccggc gctccggttc acgcggctga tccccgggga tgccgacggc
      361 gacttcgacg tgagcctagc cgcgggaagc ccccccggca tcggacctcg gggaacggtc
      421 gtcgtgaccg cgctggcccg cgtgctcacg gccgcgtcca cagggacggt ctcgatcgac
      481 gcctcggcca gcgcgatcga ggtcgtgtcc gacatcgcgg tgcaggatcc cgccgccgac
      541 gcgccggtcg tggcgttgat cacgtcgggg ggaatctcgg tgacggcggc gcaggttccg
      601 tcgagcgtgc tgccgggcgc gcgcaacgtc gtgctcgcga atctcaccgt gacgaaccac
      661 taccccgaca cccggacggt gcgctccctg cgcctcacca accgctcctc cggactcggt
      721 gaccaggatc agctggacga cgagctcggc gagacggcgc tctacaagga cgacggcgac
      781 ggcgccctgg agagcggcgc cgacacgctc ctggtcgcga cctcggccct gagcgggtcg
      841 gtcacgttcg ctcccctctc gatggcgctc gcgcccggaa gcacggcccg cctcctcgtg
      901 acgagctcca tcccgctcga tgcgcgcgac ggcgattcgc tcgacgtctc gctggacgac
      961 tcgaccgccg tgaccttcgt gcgcgttccc ttcttccgca cgggctggcc gctccggccc
     1021 cccggcgtct tcaccgtgga cggcatggcc gccgaccaga tccgcctgat gccgggcgcc
     1081 ggcgggaacc tgctcgccgg ctcgtcgaac aatccgacgc tggagttcat cgttccgccg
     1141 aacgggtacc ggcccgatct cctccagaag ctcgcggtcg tgaacttcgg cacggccgag
     1201 gccgcgaacg acatcacgcg gctgcgcgcg tgggtcgacg acggcgacag caccttcgac
     1261 gcctcgcgcg accggctgct cggcgacctc gtcaacaccg gggaccgctg gcagcgcacg
     1321 ggactctcgg agacgatccc gctcgggggg ctttgcctct tcgtcacggt ggatctgtcc
     1381 gacctcgcgc gccaggggcg caccgtgcag ctcggcatcc ccttcggcct cgacgcgggg
     1441 atcggcgtcc agagcggcaa cagcggcccg cgcgaccgcg cggtacggag cctcgccgag
     1501 cgggcggtca gcacggtgga ccgggtcacg ctcacggcgg ccgacgtgga atcgacggtg
     1561 gtccatcccg gcgaccggcg cgtgctcctc catcatttcg tgatcacgaa cagctacgcc
     1621 gatccgcgca cgctgacgag cgtcgccttc accaacgcca cgctcggccc cggcggaacg
     1681 gcagcgcggg acggggagat cgagtcggcc acgctccgcc tggacggcga cggcgacgga
     1741 ctcctcggcg atcccgcggt ggaccggccg ctcggcacct ccttcttctc gggcgggcgc
     1801 gcggcgttca cggggctctc ggcctcgatt cccgcgggcg gggccgtgca cctcttcctc
     1861 gtcgcggacg tcgcgcgaaa cggcgcccgg gatggggatg tcctgtcggc caaggtggcc
     1921 ggcccgctcg acgtgggctt caaggagccg accacgacca ccgccgcctg gccgatcgac
     1981 tcccgcgcgc gcgccacggt ggacgggctg atggccgcgc agatcacgaa catcggggcg
     2041 gcgggagtca ccatcgggcc gggcgacggg cccgtcctcg cgctgaacgt cgtcgtgccg
     2101 tggaacggtt accagaccga cacgttgcgg aacgtggcgg tggtgaatct cggatcggcc
     2161 actcccgcga cggggctcca agagctgcgc ctctggcggg acggcggcga cgggatcttc
     2221 tccgccggcg gcggagacga tgtggatctc ggcgtcatgc tccccgactc cagcgcgcag
     2281 gtctggagga gccccttcct gagcgaagcc gtggagggcg cggggcggcg gctcttcgtc
     2341 tcgctcggca cgagcgccac cgcgcgcgac tcggtcacgg tccggctcgc gatcccgttc
     2401 ggtggaatcc agatggagac gggcgacgac ggaccgctcg acgcgagggt cgagaacgag
     2461 caggcgatcc tgatcagcac ctcgccgctc ctcgcgacgc tggagacggc gagcgccgcg
     2521 agcaccgtgg gccagccgat caccgtgcgc atgatcgtcc gcaacgtcgg ggccgagacg
     2581 gtcaccggga tcctccccgg gccgcttcgc gccggcggca cggccgggac cgttctcgac
     2641 gcggctcccg gctcgccggt ggatctcgct ccggccgcgg tggacacctt cctctggacc
     2701 tatcactcga ccagcgcggg cgacgtgcag ttccagggcg gcgccgaagg cacgggcgcg
     2761 gaaaccggcc tggtccgccg ggccgtcgag acgccctcga acgtgcaccg ggtctatctc
     2821 gcggcggccg agctggcgct cttccccgtt cagtcgatgc ccttccacgt gacgcgcggc
     2881 cagaccggcg tcgtcccctt cagcctgacg ctcacgaacg gcggcggatc gggagcctcg
     2941 gacatccgcc tgcgctcgct ccgcctccac ctccaggacg agaccggcgc cggcatcgtt
     3001 cccgccgctc tcttgagcca tgtcagcgtg aacgagggaa cgaacgtcta cctgagccgg
     3061 tcgtcgctcg agacgacggg ctcggagctg aacctcacgc tcgcctcgcc gctgcgaatc
     3121 accgcccagg agccggtgac gatcagcatc cgtctcgaca tcgccgactc gacgacggtt
     3181 cccgcgttcc gggtcctgct cgccgacagc accgagatcc tggcggagga cgcgacgagc
     3241 ggcgcccccg tgaccgtgcg gcacgaggga gggtccttcc cggtcgcgtc cgggctggcc
     3301 cggctcgcgg cggacgcgac ggagctggac gtcgcctcgc tgggcggacc cgcgtcgcgc
     3361 gtcgcgatcg gcaccacggg cgtgccgctc gccgccttcc gtctcgagaa tgtgggcgtg
     3421 gcgggcatca cgtcggacgt ccaggtgaac cagatcggcg tgaccgtggc ggacaccgcc
     3481 ggcgtcccgc tggcccaggc ggcgtcgtac ttgagccgca tcgtcgtgcg cgcgggattc
     3541 cagaccttcg cgacgcagcc ggtgccggcg gggacggggc cgggcgtcac gctggtgctg
     3601 agcccgcccc tcgccgtccc ggcggggacg cccgtcgacc tgttcctcga cggagacgtg
     3661 ccggacagcg cacgggtcgg cgcgttccgc ctgatcctcc agccgccctc gtcgttcgaa
     3721 gcccaggacg cgaactcccg cgactcggtg tcggtcggct acacgggagc gccctggggc
     3781 gtggccgtcc tcgtcgaggc ccccgcgagg tccgtggcgg cccggggaac ggcgctcttc
     3841 cccgtctcgg cggtcgcggg cgagcgcgac ctcgcggcga ttcgggtcgt gctgcgccat
     3901 ccggggagcc cgggcacggg gcggatccgc gccgacagtc tcctcgtcca ggtgagggac
     3961 gagacgcgca cggcgcgcgt tcccgatctc ttcctcgatc gtctgcgcct gttccgcgac
     4021 ggcgtggagg tcttgaacgt gacgagcttc cccgtcgcgg ccgtgccggt ggcggtgccg
     4081 ctgaccgggc cgctcctgga gccgggcgac agcgccgcct tcaccctggt ggccgacctg
     4141 agtccgtcgg cgccggcgtc cttcctggag gtcgcgatcg cgggatcggg aatcctggca
     4201 tcggacgcca actcgggcca gcccgtgatc gccgcgccgg aaccgggcgt ggacttcccc
     4261 ctggtctcgg ggctcaccca catcgcttca cccgcgcgcg acctcatggc gcggctcgtg
     4321 gaccgcatgc cggcggcgct ggccgtggac gggcgtgagg tcgtgaccgg cgtcctcacg
     4381 cttcgcaacg cggcgtcggc gggctccgga tcgatccgcg tcgaccggct gcgggtgcgc
     4441 gcgtgcgacc cgggctttcg cgcgctggcc gtcggcgcgg cggcgcggcg cgtggccgcc
     4501 tacgtgaacg gcgcgctgtg ggcggcgagc gattccctcc gcgcggactc gaccgaggcc
     4561 acgctgtcgg ccgcggcccc cctcgacgtg gacgcgggcc ggaccgtgtc ggtcgagctt
     4621 cggctggtgc cgcagcccgc gccgtcggcg gcctcctttc gcgtcggcat cgacgcggcg
     4681 ggcgtgggcg tggtgcagcc gggcagcgcc cttctcacgg tgcaggtctc cccggaggcc
     4741 gggaccgcct tccccatgtg gaccgagtcg ggatcgttca cgcccgcgag cctgtccgcg
     4801 agctggggca acttcccgaa ccccttcgcc gccggccgcg aggcgacctc cttcgtctac
     4861 tacctggcct cttcggccga tgtcacgctc cggatctgga ctccctccgg cgagggggtc
     4921 gccacgctgc tggaccgggc gccgcgcggc gccggtctgc atcaggccga cgcctggaac
     4981 gggcggaacg gccgcggcga cgtggttcga aacggggtgt acgtggccga gctgatcgtg
     5041 aagtacgccg acggctcgac cgagcgggcg cggcgcaaag tggcggtggt gcgatgaggg
     5101 cacgcgcgat cgcgttcctt ctcgccgtcg cgaccgtcgg ggcggctccg cgacaggccc
     5161 gcgccgacgg tggcgacgcc ggcactcggt cccccttcgc ggccccggcc ggaacccgcg
     5221 gcatcgcgct gggcggcgcg ttcgtcgccg tgcccggcga cgtcacgatc ggcgcgtgga
     5281 atccgggcgg ccttggatgg gtcgcccggg gcgaggccag cgccagcacc gtttcctacg
     5341 acctcgggtt ccgggaggac ttcgccacgt tcgccttccc gagctggcgg ctgggcacgg
     5401 cgagcgtcgc cttccggcgc ttcggcacgg acgggatcga cggtcgcgac gaccggaacg
     5461 tatcgacggg cgaggacttc tccagctcgg agacggaggt gtcggtcgcc tacggccgca
     5521 cgttcggcac gctcgtcgga gtcggcggcg cggtgaagat gcagcgccag tcggtcgccg
     5581 gccagagcgg cagcgggctc ggcgccgacc tgggtgtcac gctctacccg ctcctcgcgc
     5641 tgcgcgtgcc cgttcgatgg gcgtcccagg ttacctgggg ggtcggcatc cggaacctgg
     5701 tcgagcccag catccgtctc gacgaggaat cggtgcgcga cccgggcacg cgccggatgg
     5761 ggttcgccta tcgcacggcc ttcggcctcg ccacgctcga tgtcgagacg ggaggaggca
     5821 tccggccgcg cctccacgcc ggcgccgagt atgcgccgcg cccctacctc tccgtccgcg
     5881 ccgggatgaa cggcggcgcg cttacggccg gcgcgggctt ccgggcgcgg ggggtcgcgc
     5941 tcgactacgc cttcgaggac cggggctacg atccgatcca ccgcgtcggg ctctcctacg
     6001 cgttcggtcc cacgacggcc gagcgccggg acgccgcact ccgggccgag gatgaaaagc
     6061 tgcaggcgcg cctggccgag accttccagc gcgtccagcg ggagaaggtc cgggggcttc
     6121 tgacgcgggc cgaggaagcg cgcgcggcgg gctcctacgc cgaggcgctc gaggcactgg
     6181 ccacgatcgg cacgctcgac ccggacaacc cggaagccgc cgacctcgag atccgctgtc
     6241 tccgggagca gggggccacg ttcgagcagg agggaaacct ggccgaggcg gcgatggcgt
     6301 acgggcgcat cctcgaaaag cggccgcagg atcccgacgc gcgcgtggcg caggggcgct
     6361 gccgcgccga gagcgaccgg cgcgccgcac gctcggccga gatccggaag tccttcgccc
     6421 aggcgatgga cgcgttcgct tcggacaacc tcgtggccgc gcgggacggc ttcgcggcca
     6481 tcctgcgcga gacgcccaac gacgacgacg cccgggcgat gctgcgccgc acccagacgg
     6541 ctctctcgcg gcgcgcccag ggaatcctgc gcgacgcccg ccgaagcctc gccgaccgcc
     6601 atctcgacgc ggccgccacg cagctccagg aggcgcgcgg tctcgatccc ggcgccgacg
     6661 gactcgcctc cctcgcagcg gcgctggaac gggctcgctc caccgcctcc gcgcgcgcgc
     6721 cggagccggc ccggtcggcc ccgccgccgg cgccttcggc ccccacgctc cgcccgggcg
     6781 agcttcgtga gttctaccgc agggcgctgt ccgccctggc cgaaaagcgc tcggccgacg
     6841 cgctgcgcta cttcgagctc gtctggtcgg cggatcccaa gtatcagcgc gtggccgaat
     6901 acctggagcg cgagtacctg atccagggcc tcgacgccta cgcggccgga cgcctggacg
     6961 atgcgatcgc gcactgggag cgcgcgctcg ccgtcgaccc cagcgacccg cgcgcgcgcg
     7021 gctatctcac gcgcgcccag cagcagctgg agcgctcgcg cgagatggcg ggggatgcgc
     7081 cgtgatcggg cgcggcgaac ggatcatcgg catccgctgg atcgtcacgt tcgtggcggt
     7141 cggcctgacc acggcggtcg tgctgagcgt cggaacggtg gccgagcgga acgcgcggcg
     7201 ggcattgacg gccgaactga ccagccgcct cctcctcgag gcgcggaacc ttgccctgac
     7261 cagctccggg gtgctcttga gcgatttccc ggagctgacc ctccacccgc tgatcaagga
     7321 gaaactcgcg cggcagcccg agctctcgta cgccgtggtg gtcgaccggg ccgggatcat
     7381 ccaggggcac gccgatccgc accgcctcgg cacgccgttc gaagagccgg tcggcctgcg
     7441 cgccctgatg gacggcccgc cgcgatcggc cggggaaacc cttcggcgaa acgaccgcga
     7501 gctggtcgct tcggtgccga tcacccatcc ctcggggaaa cggctggggc acgtcgtcgt
     7561 cgccatgcgc acgagctacg tggacgcggt cctcgagcgg gctcgccggc agcagtcgct
     7621 gatcctggcg atctcgctcc tcatcgggat cgccgtctcg ttcctggtcc tctcgcacgt
     7681 cctccggccg gtcgcgaccc ttcgcgaggg aatccggcgc atcgcgggcg gcgatctcga
     7741 gacgcccgtc cgcgtgagcg acgggaccga gctgggtctg ctcgccaacg cgatcaacga
     7801 catggcggcc ggcctgaaga aggcgcagat ggagatggtc gagcgggagc gcctcgtccg
     7861 ggagatggag ctggcccgcg agatccagga ctccatcctc ccctcggagc ggatcgtgga
     7921 aggcccgttc gtcatcgagg gctcccagcg cgccgcggcc gaagtggggg gcgactacta
     7981 cgactacttc ctgcttccgg acggaaggat cggattcgcc gtcgctgacg tctcgggcaa
     8041 gggcgtcgcg ggctgcctga tcatgacgat gctctatgcg ctcctccggg ccgaccgcgc
     8101 gacggcctct tccccggcgg cgctcctcgc ttcgctcgac gagcgcctcg gcgagacgct
     8161 ccgccgcgga agcttcgtca cgatgttcta cggcatcctg gacgttcgca cgggccggct
     8221 cgtctacgcc tcggccgggc acaatcccac gttgatctac cggcacgcga gccgctccgt
     8281 ggagaccctc cgctcccgcg gcatcccgct cggagccatc cggggcggcg cgatccgagg
     8341 cacgctcgag gatctgacga tcacgctcga gccaggcgac gcggtcctcc agttcaccga
     8401 cggcatcagc gaggcgttcg aggcgtccgg ccaggagcag ttcgggcacg agcgcatgag
     8461 cgcgacgctg cgcgacaccg cgccccgcgg gccgcgcgcg gtgatcgacg gcttcttcga
     8521 gcggctcacg gcctggacgg gcggcggcgc gcgcctggac gacgagacga ttctcgtgct
     8581 ccatcgagac ccggcccacg tccccgcgcc cctcgagagc gagccggtcg gaaacggctg
     8641 cgggagtccg ctcccgaccc gggagcccga tcccggcgac agcgcaaccg cgatcgcgcg
     8701 ctacgacgag gcgcggcgcc gcggttcgcg cgtggtcctg accggcacct cggacgtgct
     8761 cgacacgatc cgcggctggc tcgcgggcac gccggtcctg gacgccctgc ccgcgcgcga
     8821 acgcgatctc ctgacgagca ccctctacga agtctgcgcc aacgtggccg agcacgggta
     8881 cgcgcgcgat cccgggcggg agtacgaggt ctggtgggtt cccgcccccg ggcccgcgga
     8941 aggggcgcgc tccccgcgtg cgcgcgccgc ggccgggacg gcgtcggagc gtctccgtca
     9001 gggctggttc ctggtccgcg acgaaggagt tcccttcagc gccaacgaat ggaagggcca
     9061 ggatttcgac gacccccgcg tccggcggcg cggacgcggc ctggggctcg agatcatcca
     9121 ccgcaccatg cgccgaatcg tctactaccc gtccaccgcg gtggggaacg tcacctggat
     9181 gtgctttggc ggctcaccgt ctccggagga ggcatcgcat gccggatagc tttgggattc
     9241 aggaagagac ccgcggaagc gcggtcctgc tgcgggtccg cgggcgcctg gacgcccgca
     9301 gcgcgcccca cctcctggag gtcggatcca gggtgcggat gcgcgggcga aacctcgttc
     9361 tcgatctctc gggagtcacg ttcatggctt cgagcggact ggggtccctg ctcagcctgg
     9421 ccgaggaatt ccgccagacc gatttccggc tgcggatcgc gtccccctcc gcggcggtga
     9481 cgtcggtgat ccagctcctg aacctccagc cgttcctgtc gatcgacgcg tccgaggagg
     9541 aggctctgca tgccctcgga gtctgatccc gtcatcccgc gacccgtcga ggcgtccacc
     9601 gtgccgtccg gaccgggccc ctcggacgag gcgacgcgtg cgctccggct ggtggcccgg
     9661 cgggagcatg agctgctcgc gctctccgag ctgagccagg agctgacgat ctccctcgac
     9721 ctgtacggcg tggccgacgt ggtgctcttc aatctcatgg gacagctcgg aacttcgcgg
     9781 gcggcgctct ggctgatgtc cgagcacggc cgcgccgtcc cggtgctgct ccggagccac
     9841 gggatcgatc gacgcacggc gtccgcgctg ggcagcgcct gctcggcctg gctcctcgag
     9901 cgcttcgccc aagagccgcg catcgtgctc gcggccgaga tgatcgagga gccggcgcgc
     9961 gccgcctcgc tcctcgcacg gcgggaatcg atcacgctct tcgctcccat cctggcgcgc
    10021 ggcgaagtgc tggggttcat cgcgctgggc ccgcgcgtcg gtcccgggac atacggcgcc
    10081 gtagagttgc aggggctgca gacctccctc ggcatggtgg gcgtcgcgct ccacaacacc
    10141 cggctctaca accggcttct ggagaacaac cgccggcttc ggctggcgaa cgacaacctg
    10201 atggagttgg accggctgaa gtcggaattc ctgagcaacg tgaaccacga gctgcgcacg
    10261 ccgctcaccg tggtgatcgc gtcccttcag atcgtgctcg agcacgacgc tcccgacccc
    10321 caattggccg agttcctgac ggccgcgctc ggggaggcgc agagcctgaa gggaagcctg
    10381 gagaagctcc tcgacctgtc cgacctgcgc cggaacgtgg tgccgttcca gttcgagacg
    10441 ggcgacgtcg cggcgccttt gaggggctac tacgaggagc ggctccccgg cgtgacccag
    10501 gggctccggg agctggcgtt ccacgcgatg cccgacctgc cgcccgcccg gttcgaccgc
    10561 caccgcatgc tgcaggtcgt cgacgccctc gtggacaacg cggtcaagtt caccccggag
    10621 ggatcccgca tcgatctcgc cgtcaggacc gccgagcgcg aggggcggca ctggatccgc
    10681 atcgacgtcg cggacgacgg gccgggaatc gccacggacc acatgagcgt tctcttcgaa
    10741 tcgttcagcc aggtggacgg ctccgcgacg cgcaaggtcg gcggcatggg gctcgggctc
    10801 gccgtggcgc gacagctggc cgagcggatg gacggggcgc tgacggcggc cagcaagccg
    10861 ggtcaaggct ccgtcttcag cctgtacctg ccgctggagt aacccgccgc cggtctgccg
    10921 ctggagtcac cgccgccggt gcgggtccgg gcgcggtggc gcgctgggtt agaatgcccg
    10981 catgcgccat ttccggaaga ccctcacgtt cgagatcccg gcccgcatgg agttcgtcaa
    11041 catcacgccc gaggtggagg cggcgctgcg cgagagcggc atcgccgagg ggctctgcct
    11101 ggtgaacgcg atgcacatca ccgcgagcgt gttcatcaac gacgacgagt cgggtcttca
    11161 cgccgactac aagcgctggc tggaagagct cgcccccttc gacgcgagcc cggcgcgcta
    11221 ccgtcacaac cgcaccggcg aggacaatgc cgacgcccat ctgaaacgcc aggtgatggg
    11281 gcgggaagtg gtcgtggcgg tgacgaaggg gaagctcgac ttcgggccgt gggagcagat
    11341 cttctacggc gagttcgatg gaaggcgagc caagcgggtg ctgatcaaga tcatcgggga
    11401 gtagcggatc cggcggccgc cccgcccggg aaaaaacggt cgacaccgaa aatcccgggt
    11461 gtagtctcct cctctaagct ccccgagagg acgccgatgg tagccagtgc ccgacaaacg
    11521 gcgcgcgact acacggtcgc gctttccgtg accgcgctcg ccatcatcgt gcgcggagcg
    11581 atcgatcctc tgctgggcga ataccagccg ctggcccttc tctacgcctc cgtcgcgatc
    11641 accgtgtggc tggccgggct ctacccttcc gtggtcgcct gcgtcgccgg ctacgtcgcg
    11701 tccaacatct tgttcatcga gccgcgcggc cgcttcgcgc tcccgcatgc gcatgactgg
    11761 ctggccctgg gcggatatct cctcgccgcg tccgtcctga tcttcgtggg cgagagcctg
    11821 cggaggaacc gcgccgtgct gcgcgagcgg gtcgacgcgg ggatccgcat ggagcgggag
    11881 ctttcggcgg cccgggatca gctcctcatc gtgaccgact gcatgtccgc gctggtcctg
    11941 cactgcaacc gagccttcga atgcacgtgg gtcagcaaga cctacgcttc ctggctcggt
    12001 cttcggccgg aggagatcgt cggccggtcg atcgaagacg tgatcggccg cgaggccttc
    12061 gagcagctcc gtccccatct cgagcgcgtc ctccgcggtg agatcgtacg ttgtgaggag
    12121 gaaatgccct tcccgggaca atcgtcccgc tggatcagcg ccgtctatac ccccacccgt
    12181 gacgcccatg gggagccgga gggctgggtc ggcgtcgtca ccgacatcga ccagcggaag
    12241 cgcgaggaac aatcgctgcg cgaagccgac cgccggaaga acgagttcct ggcgctcctg
    12301 gcgcacgagc tgcgcgcgcc gctcgcgccc atccgaaacg ccgttcgatt tctgcagatc
    12361 gcggcgccgc agcagggaga gctccaggcg gcgcgcgaca tcatcgaccg gcaagtggac
    12421 cagatgatcc ggctgatcga ggacctcctc gacatttccc gcatcaccca gggccggatc
    12481 cagatccgca agtcgaggat ggagcttggg ccggtggtgc agctcgccgt ggaggcatcg
    12541 cggcagctca tcaacttgcg cgatcagagc ctgacggtga cggccccctc cgagccggtg
    12601 tggctggaag ccgatcccgc ccggctcgcg cagatcctgt cgaatctcct ctacaacgcg
    12661 gcgaaataca cggatcccgg gggatgcatc ggcctctccg tgaagcgcga gggccacgaa
    12721 gtggtcctct ccgttcgcga cacgggcgtg ggcatccgga aggaagatct cggcgcgatc
    12781 ttcgacatgt tcgcccagcg gcccctcgat ccgagaaatg ccgtcggggg gctgggaatc
    12841 ggactcgccc tggtccgtgg gttggtggag ctgcacgagg gctcgatcga ggcccgaagc
    12901 gcgggccccg ggtgcgggag cgaattcatc gtccggcttc ccgccccgcc atcgcgaggc
    12961 acccacgccg cggccgaagg cgaggtcgcg gccggggacg gagcttccct ccggatcctg
    13021 atcgtggacg acagccgcga cacggcggac agcctgcgca tgctgctttc gctggggggg
    13081 cacgaggtcc ggacctccca tgacggggtc caggcggtga ccgaggcgga ggagttccgg
    13141 cccgacctcg tcctgctgga catcgggttg cccctcatgg acggctacga ggtcgcgagg
    13201 gagatcagga gccgaccgtg gggtccgaag accgtcctcg tcgcgctgac ggggtggagt
    13261 cgcgacgagg atcgcattcg cacccgtcag gccgggttcg attaccacct cacgaagccg
    13321 gccgatccca aggagctgga ggccctcgtc gggcgcctga ccggacgggc cccgacgacc
    13381 ggcgggctcg aagccggcga agctcgaggc gccgaggcgc cccaccccgg taccccaatg
    13441 ggcgccttct agcgccgctc ggccaggagc atcgcggccg cgagatgctc cgcgatctcc
    13501 agccgcgata ccgcctcctc cacgttggtg cccaccgaga tcgccccgtg gttctgcagc
    13561 accagcaccg tcggcgcgac gactcccggc ggatccagcg cgagcccgat cgcctcggcc
    13621 aggtcgtcgc ttcccgacgc gtggtagcgc acgaacccga cgtgccccag ataggcgcgc
    13681 gcttcgtcga tgcgcgagaa gtcgggagcg tgaccgcgca gcgcgaacgc ggtgagcgcc
    13741 acggggtgcg cgtgcacgat cgcctccacg tcgcgccgca cggcgtaggc cacgagatgc
    13801 acgcggtgtt ccgtcgaggc ggcgccgcgg gccgccgagt cgcgcggctc catcagcgtg
    13861 agctccacga agtcgcgcgg ggtcaggtct cccttacgtt tcccgcgccg cgtgatcagc
    13921 acgcggtccg ccgtcatccg cgcgctcagg ttgccttcgc tggcggcgag catcccccgg
    13981 cgatccagaa tgcgcccggc ccgcaccagg tcgtgggcca gcgcgatccg cacggctcgg
    14041 ttcttggcgg ccgcggagcg ctcggcgttc ggcccggcgc cgcgccggcg gccgtcggga
    14101 tcctcgcgtc cggggccctt ggggttcatc gcgccgtgtg gaagcagtgg cgggagacgc
    14161 cgctcatcgc gcctccggct cttcgatcgc gccgatctcc agcgcgacga gcgccgccgg
    14221 gatcgcctcg acgatgtcgg tggcgatcat tccgcgcatg cccttccgtc gcgccgccac
    14281 gtcgccggcg agcccgtgca cgaagcatcc caccgcggcc gcctcgggcg gatccatccc
    14341 ctgcgccaag agtcccgaga tgaccccgga gagcgcgtcc cccgccccgg cggtggccat
    14401 gccggggttg ccgctcgcgt tcaggatcag cggctcggcg ggggccgccg tcagcatggg
    14461 cgtggatttc aggcacacga cggcgttgct ctgcgcggag aagcggcgcg cccatcccat
    14521 ggggtcgcgc gccacctcgg cgatgagccg cccggtcagg cgggccgcct cgccatagtg
    14581 gggcgaaacg agaagcggcg cgtgcaggcg ccggagcggc ctcccctcga acgcgttcaa
    14641 gccgtcggcg tcgagcagga tcgggcactc gacccgctcc accagggccc gcaccagatc
    14701 ggcggtctcc ggatcgcgcg aggcgccggg tccgatcagc atcacgtcgg cgtggagcgc
    14761 ctcgttcagg atcgggtcca gcgcgtcgag cgcgagcgcg cgagccggcg tctccggcat
    14821 cccgcgcttc accaccgcgg ggacccgcga ttccaggacg tccacgcagg agcggggcac
    14881 ggccgcgacg gcataccccg caccggcgcg aagcaccgac tccgaggcca tcgccaccgc
    14941 gcccatcatg ccgccgctcc cgcccaccac gagcacgcgc cccatcgagc ccttgtgcgc
    15001 gtccgcggcg cggcgcggca ggagggagcg cgcccagccc gcgtcggcca tgaggagcga
    15061 ggcgggctgt tcggcgacga ggtcgggcgg gatcccgatg tccaccgcct cgatccggcc
    15121 ggcgtgggtc cgcccaggct ccagcaggag gcccgtcttc atgaccgcca gcgtcaccgt
    15181 gaacgcggcc cggatcgcgg gctcctcggc gtagccggtg gacccgtcca ccccggaggg
    15241 gagatcgatc gaggcgatcg gacgccccga ggcgtccgcc atctcgatcg cggccccgat
    15301 cgcccctcgc ggcgcgccgg tcgacccggt gccgagaaga gcgtcgacga ggaaggcggt
    15361 gccgcgggcg gcgagcgcca cttcgcttcc ggggccccac ccgcgggggt ccggcagaaa
    15421 ggtgatcggg atgcgcgct
//
LOCUS       DASSLZ010000015        11556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1400547, whole genome shotgun sequence.
ACCESSION   DASSLZ010000015 DASSLZ010000000
VERSION     DASSLZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400547"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFM00_00510"
     CDS             <1..258
                     /locus_tag="VFM00_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005522543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET7496759.1"
                     /translation="APPPRGVASRPEPAGALEGPGAPAPPPQRARARETHAPVTAFGT
                     PAEETPSKERRAPVATQEPKVGRNDPCPCGSGKKYKKCHGA"
     gene            274..744
                     /locus_tag="VFM00_00515"
     CDS             274..744
                     /locus_tag="VFM00_00515"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HET7496760.1"
                     /translation="MEDSVREMMVLIVEQLKSYVDGNEDALLELTQVLDSGRYDVEIV
                     NQAFEMIFRALEPFAREDWSDEAVATRKSVRVPTGSERALLPSAAYKYLFGLVGSGKV
                     TPEQFEEIMDRARDEGQALDTEAKAEELATSVLIRWFDDEHGVDFDPTYPAQVH"
     gene            761..3211
                     /gene="topA"
                     /locus_tag="VFM00_00520"
     CDS             761..3211
                     /gene="topA"
                     /locus_tag="VFM00_00520"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET7496761.1"
                     /translation="MTKKKMPAAAPEADAAAIPVTKIPAGAKKKTAAKKKTAARGTKK
                     AAAAPESDGKPARRGRNLVIVESPAKARTIAKYLGPGYEVKASNGHVRDLPKSKLGVD
                     VDKGFVPSYVLIKGKGKIIKDLKTSARGAAIIYLAPDPDREGEAIAWHLAETLGTDGR
                     AGDADGRIRRLAFYEITKRGIEEALASPREIDMSKVHAQQARRILDRLVGYQVSPFLW
                     KTVRYGLSAGRVQSVALRLICEREDEIRAFVPREYWTLDADLETPKGETFRARVQKKN
                     GEKFAIGNEADAKAEEAALAKESFEVTGIRTQEKKRNPLPPFITSTLQQEAFRRHRYS
                     AQRTMVIAQQLYEGIDLGAEGSTGLITYMRTDSTRVSPDALAEVRGFIQAQYGDAYLP
                     AEARAYRSRETSQDAHEAIRPTSVARTPASMKHHLDPDQAKLYELIWQRFVASQMNPA
                     LVLTTTADITAGPYLLRASGSRVKFDGFARAYQTALIEGDSPGAAPSRLPALEQGNAL
                     KLLTTVPEQHFTEPPPHYTEATLVKTLEEKGIGRPSTYATIVGTILSRDYVERDRGKL
                     LPTELGMTVWKLLSKTFSDVFDVEFTAKLEERLDHVETGRDPWLEVVEEFYHPFQKDL
                     ASAESKQEQVRASLVQETETPCPKCGSKMIKKFGRSGPFLACPRYPECKTTLPVEEDG
                     VPAEAPTQLCPQCGGPMRFRTGRFGKFLACERYPDCKGTRPFTLQIACPQCGTGEMVE
                     RRTKRGKIFYGCSRYPECTFAIWDKPVKETCPSCGSTIMVQKRSKTKGDYLQCPKCKT
                     QVDQDAVAGDALSGTLDG"
     gene            3192..4100
                     /locus_tag="VFM00_00525"
     CDS             3192..4100
                     /locus_tag="VFM00_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7496762.1"
                     /translation="MERSTAEFLAHLADRRYAALTVRAYGEELLRLREFLAGRLGRPA
                     KPADLTPDLLTAFAAARSLERTAARRPISARTLARTLAAVRCYLRYLERRGKVSASAG
                     AGLPRVTLPSPLPSAHAVEPLNQLLDEIAAKIPAQDARGCRGLAAAELLYGSGLRVGE
                     LASLTRDRVDERRMLIRVLGKGSRERVVPVGQRALAALRRFWALAGGEPRPGDPLLRG
                     RSGGVSARTLERDVQSLLARLGPNAATHPHALRHSFATHLLDAGANLRAVQELLGHQN
                     LRTTQIYTHVTRRRLKEAYARAHPRA"
     gene            4141..5223
                     /gene="argF"
                     /locus_tag="VFM00_00530"
     CDS             4141..5223
                     /gene="argF"
                     /locus_tag="VFM00_00530"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET7496763.1"
                     /translation="MKRDLLSFADLTRADADRLFATAARLKSDLRAGRRHLELAGRTL
                     ALIFHKPSLRTRVSFEVAMTQLGGSSIFITDREIGMGSREPVQDVARVLSGYVDGIMI
                     RTFDHQIAVNLAKHASVPVVNGLTDWLHPCQILADMLTMIEHGLDLDGAVVAYVGDGN
                     NVANSWIEAAGLYGLTLRIACPEGYDPDMALVAQVRNTGKGRVEIVRTPDEAADGADV
                     LYTDVWASMGQEAEREKRLPVFRPYQINGALLDRAKPNALVLHCLPAHRGEEITDEVM
                     EGPNSGVFPQAENRLHVQKAVLYELLVAGATGRRSGAGRAAPARKPAARKKAAGKSAG
                     RKPAARKPAARKPTARRKAARKRPRR"
     gene            5220..6293
                     /locus_tag="VFM00_00535"
     CDS             5220..6293
                     /locus_tag="VFM00_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7496764.1"
                     /translation="MIDGRGRGLLRALLLGALLAAIGCAKSVFPPGGPLDVTPPRVIA
                     TTPADSSVGVDRNTGVDFLFSEDMDHGSVRDATRIRPSVERPQFRWSGRHFRVFWGET
                     LRVNTTYHVVLRGSVRDAHGVLMGAPVSIHFSTGDSLAPGKITGVLRARTLGRSGVPI
                     LVFSDSLGARPDTGVTFEPLYEAATDTAGVYSIPGMILGPEYRVFAFYDRNGNNTYDE
                     GQDVLAPHQEPIRLTPERPVADSINIVAVDPRALAVLTGTIVASDSTARYRVEARGAA
                     DSTVTAPPVQRIGPGAFTMRVPAGTWRLRAFRLEGLSGSPPRLELRWPEAVVLRPEEE
                     RGGFTFDFRPAEGPGAPPPPPKR"
     gene            6323..7228
                     /gene="dapA"
                     /locus_tag="VFM00_00540"
     CDS             6323..7228
                     /gene="dapA"
                     /locus_tag="VFM00_00540"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET7496765.1"
                     /translation="MSERTGIFEGVGVALVTPFRGGAVDREALAALAASLVARGVRAL
                     YPCGCTGEATSLTREERAAVIRIAVEAARGKAAVVAGTGTAVTDETIEFSREAIRLGV
                     DAVMVITPYSIRPTQEGLIAHYRAVADAIDRPMVLYNVPTRTGANILPETVARLGEHP
                     RIAAIKEASGSLDQASQIRGRSDITILAGDDALYLPLLSIGAKGVVSVAGHLTPEDLV
                     LMGSHYRAGRVAEAEAIHRRLYPLFKALFIETSPAPVKYALSALGSIAPDLRLPLVGL
                     KPETIRAVDAALDGLGLRAPARAAH"
     gene            7233..8012
                     /locus_tag="VFM00_00545"
     CDS             7233..8012
                     /locus_tag="VFM00_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HET7496766.1"
                     /translation="MGGSAAVVPILLFGATGRMGRAIRASIAASAASERGTFSLRGCV
                     ARSHDGEPHPEGCVWFGPDALAKPEGLASLPPDLVVIDVSLAAGTEALLGALEASPRA
                     LVAATTALSAATEERIARLAGRAPILRARNLSPGIAVLAALLRALPPGARGLYDVDVL
                     EHHHAAKKDAPSGTAIALGELLGGVGTERRPGGVAMHSIRGGSAPGTHEIVLSGEGEV
                     LTLGHMVHDRAVFARGALRAARFLHGRKPGLYSFEDALTEA"
     gene            8029..9747
                     /locus_tag="VFM00_00550"
     CDS             8029..9747
                     /locus_tag="VFM00_00550"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET7496767.1"
                     /translation="MRFSQAFIPTLKEDPADAELVSHRLMIRAGMLRKHAAGIYSLLP
                     LGWRTVRKVSQIIREELNREGAQEISLPILMPSELWMESGRWQKHGKELFRQKDRHDR
                     DYALGPTNEEAVVDLVRNEVRSYRDLPLNLYQMGIKFRDEVRPRFGLMRAREFLMKDG
                     YSFHADESSLEEGYEAMRRAYTAIFRRCGLSFTMVEADSGAIGGDVNHEFMVTAESGE
                     SEVFSSACGYAASSERAKFALPPAPHEPEAALAERETPGKKSVEEVAAFLGVTSDRLL
                     KTLVFWAGGDPILAVVPGDRELNEAKLSRYLGGAPVRLAAAAEIEQLTGGPMGFTGPV
                     GLETRIRTIWDPSVQEGKNWVAGGNKKDVHLVNVRLGRDAAAKERADVATARRGDRCP
                     RCGDELVVSRGIEVGHIFKLGTRYSEPMKARFLDAAGAEKTIVMGTYGIGITRTVAAV
                     IEQSHDENGIVWPMPLAPFHVHVLAVNQKHEASRETAEKIAADLEARGVEVLLDDRDE
                     RPGAKFKDADLLGMPLRVTVGERGLAEGVVEVRDRKAGAVEKVPVAEASGIVARRVQE
                     ALRSSE"
     gene            complement(10709..10963)
                     /gene="rpsT"
                     /locus_tag="VFM00_00555"
     CDS             complement(10709..10963)
                     /gene="rpsT"
                     /locus_tag="VFM00_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET7496768.1"
                     /translation="MPHHKSAVKRMRTTKRDRTKNIAVRSELKTLLKRMADQPANTDV
                     MKATVSELDRAVKKGVIKKAVANRRKSRLAKAANRTKAAK"
     gene            11082..>11556
                     /locus_tag="VFM00_00560"
     CDS             11082..>11556
                     /locus_tag="VFM00_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET7496769.1"
                     /translation="MAPSTLESQIAALPAQPGVYIFRGAKGELLYVGKAKRLDHRVRS
                     HFANPEQIGPKQVGLIRRVTDVEYIAAESEIDALLLEATLVRERQPPYNIRLKDDKRY
                     PYVRVSWQEPFPRVTLVRRVEKDGARYFGPYTEVSALRSLLRHIQTIFPIRTCRDI"
BASE COUNT         1857 a         4007 c         4036 g         1656 t
ORIGIN      
        1 gccccgccgc cgcgcggcgt cgcgtcgcgg cccgagccgg ccggcgcgct cgagggcccg
       61 ggcgcccccg cgccgccgcc gcagcgcgcg cgcgcgcggg agacccacgc gcccgtcacg
      121 gcgttcggaa ccccggcgga ggagacgcca tccaaggagc ggcgcgctcc ggtggcgacg
      181 caggaaccca aggtcggtcg aaacgacccc tgcccgtgcg gcagcgggaa gaagtacaag
      241 aagtgccacg gagcctagct caggaggaac acgatggaag acagcgtccg tgaaatgatg
      301 gttctcatcg tcgaacagct gaagtcgtac gtcgacggga acgaggacgc gctcctcgag
      361 ctcacccagg tgctcgactc cggccgctac gacgtggaga tcgtgaacca ggcgttcgag
      421 atgatcttcc gcgctctcga gccgttcgcc cgggaggact ggagcgacga ggcggtcgcg
      481 acgcgcaaga gcgtccgcgt gccgacgggt tccgagcgcg cgctcctgcc gagcgccgcc
      541 tacaagtacc tcttcgggct ggtcgggtcg ggcaaggtca cccccgagca gttcgaagag
      601 atcatggatc gcgcccgtga cgagggccag gcactggaca ccgaggcgaa agccgaggag
      661 ctggccacgt cggtcctgat ccggtggttc gacgacgagc acggcgtcga cttcgatccg
      721 acctatcccg cccaagtcca ttgaggttcc gttgactcct gtgaccaaga agaaaatgcc
      781 cgccgcggcg cctgaagccg atgccgcggc gatcccggtg accaaaatcc ccgctggcgc
      841 gaagaagaag accgccgcga agaagaagac ggccgcgcgc ggaacgaaga aggccgccgc
      901 cgcgcccgag agcgacggga agcccgcgcg ccgggggcgc aatctcgtca tcgtggagtc
      961 cccggccaag gcgcgcacca tcgccaagta tctcggcccc ggctacgagg tgaaggcctc
     1021 gaacgggcac gtgcgcgatc tccccaagtc caagctcggc gtcgacgtgg acaagggttt
     1081 cgtcccgtcg tacgtcctca tcaagggcaa ggggaagatc atcaaggacc tgaagacgag
     1141 cgcccggggc gccgcgatca tctatctcgc gcccgatccc gaccgcgagg gcgaggcgat
     1201 cgcctggcat ctcgccgaga cgctcgggac cgatggccgc gcgggggatg cggacggccg
     1261 gatccgccgg ctcgccttct acgagatcac gaagcggggc atcgaggagg cgctcgcgag
     1321 cccccgcgag atcgacatgt ccaaggtcca tgcgcagcag gcgcggcgca tcctggaccg
     1381 gctggtgggc taccaggttt ccccgttcct ctggaagacg gtgcgctacg gcctctccgc
     1441 cgggcgcgtg cagtccgtgg cgctgcgcct gatctgcgag cgcgaggacg agatccgcgc
     1501 gttcgtgccg cgcgagtact ggaccctgga cgccgatctg gagacgccca agggggagac
     1561 cttccgcgcc cgcgtccaga agaagaacgg cgagaagttc gcgatcggga acgaggccga
     1621 cgccaaggcg gaggaggcgg cgctcgcgaa ggaatcgttc gaggtcacgg ggatccgcac
     1681 gcaggagaag aagcggaacc cgctcccgcc cttcatcacg agcacgctcc agcaggaggc
     1741 cttccggcgc caccgctaca gcgcgcagcg cacgatggtc atcgcgcagc agctgtacga
     1801 gggcatcgac ctcggcgccg aggggtcgac gggattgatc acctacatgc gaaccgactc
     1861 gacgcgcgtc tcgcccgacg cgctcgccga ggttcgcggc ttcatccagg cgcagtacgg
     1921 cgacgcctac ctgccggccg aggcgcgggc ctatcgctcg cgcgagacct cgcaggacgc
     1981 gcacgaggcg atccgaccca cgtcggtggc gcgcaccccg gcctcgatga agcaccattt
     2041 ggatcccgac caggcgaagc tgtacgagct gatctggcag cgcttcgtgg cgtcccagat
     2101 gaatccggcg ctggtgctca cgaccaccgc cgacatcacg gcggggccgt acctgctgcg
     2161 cgcttcgggc agccgggtca agttcgacgg gttcgcccgc gcctatcaga ccgcgctgat
     2221 cgaaggcgac agcccgggcg cggcgccgag ccgccttccc gcgctcgagc aggggaacgc
     2281 cctcaagctc ctcacgaccg ttcccgagca gcacttcacc gagccgccgc cccactacac
     2341 cgaggccacg ctcgtgaaga cgctcgaaga gaaggggatc gggcgcccca gcacctacgc
     2401 caccatcgtc ggcaccatcc tgagccgcga ctacgtcgag cgcgaccggg ggaagctcct
     2461 cccgaccgag ctcgggatga ccgtctggaa gctcctctcc aagaccttct ccgacgtgtt
     2521 cgacgtggag ttcacggcga agctggagga gcggctcgat cacgtcgaga cggggcggga
     2581 cccctggctc gaggtggtgg aggagttcta ccaccccttc cagaaggatc tggcgtcggc
     2641 cgaatcgaag caggagcagg tgcgcgcgtc gctggtccag gagaccgaga cgccctgccc
     2701 caagtgcggc tccaagatga tcaagaagtt cggccgctcc ggaccgttcc tggcctgccc
     2761 ccgctatccc gagtgcaaga cgacgctgcc ggtcgaggag gacggcgtgc cggccgaggc
     2821 gccgacgcag ctgtgcccgc agtgcggcgg cccgatgcgc ttccgcaccg gccgtttcgg
     2881 caagttcctc gcgtgcgagc gctaccccga ctgcaagggg acgcgcccct tcacgctcca
     2941 gatcgcctgt cctcagtgcg gcacgggcga gatggtggag cggcggacca agcgcgggaa
     3001 gatcttctac ggatgcagcc gctatccgga gtgcacgttc gccatctggg acaagccggt
     3061 gaaggaaacg tgtccttcgt gcggatccac gatcatggtg cagaagcgga gcaagacgaa
     3121 gggggactac ctgcagtgtc cgaagtgcaa gacgcaggtg gaccaggatg cggtggcggg
     3181 ggacgctctg agtggaacgc tcgacggctg aattcctcgc gcacctcgcg gaccggcgct
     3241 acgcggcgct caccgtccgc gcgtacggcg aagagctgct ccggctccgc gaattcctgg
     3301 cgggccggct gggccgcccc gcgaaacccg cggacctgac gcccgatcta ttgaccgcgt
     3361 tcgcggccgc gcgctcgctg gagcgaacgg cggcgcgccg ccccatctcc gcccgcaccc
     3421 tcgcccgaac gctggccgcg gtgcggtgct acctgcgcta cctggaacgg cgcggcaagg
     3481 tatcggcgtc ggccggcgca gggctgccac gcgtcacctt gcccagcccg ctcccctcgg
     3541 cgcacgcggt cgagccgctc aatcagctcc tggacgagat cgccgcgaag atcccggccc
     3601 aggacgcccg gggatgccgc ggcctggcgg ccgcggagct cctctatggc tcggggctcc
     3661 gcgtcggcga gctggcctcg ctcacgcgcg accgcgtgga cgagcggcgc atgctgatcc
     3721 gcgtgctggg gaaggggagc cgcgagcgcg tcgtcccggt gggacagcgc gcgctggcgg
     3781 ccctccgccg cttctgggcc ctcgcgggcg gggagccgcg tcccggcgat ccgctcctgc
     3841 gcgggcgctc cggcggcgtg agcgcgcgga ccctggagcg cgacgtccag tcgctgctcg
     3901 cgcgcctggg accgaacgcg gcgacccatc cgcacgcgct ccgccactcg ttcgccacgc
     3961 acctcctgga cgccggcgcg aacctgcgcg cggtccagga gctgttgggt caccagaatc
     4021 ttcgcaccac gcagatctac acccacgtca cccggcggcg cctgaaagag gcctacgccc
     4081 gcgcgcatcc gcgcgcatga gccccggctt ggcaccgata ccccacgagg agcctgacgc
     4141 atgaagcggg acctgctgtc tttcgccgac ctcacgcggg ccgacgccga ccgcctgttc
     4201 gccaccgcgg cgcgtctcaa gtccgacctt cgcgccggcc gccgtcatct cgagctggcg
     4261 gggcgcaccc ttgcgctgat cttccacaag cccagcctgc gcacgcgcgt gtcgttcgaa
     4321 gtcgcgatga cgcagctggg cggatcgtcg atcttcatca ccgaccggga gatcgggatg
     4381 ggatcccgcg agccggtgca ggacgtggcg cgcgtactgt caggctacgt ggacggcatc
     4441 atgatccgga cgttcgacca ccagatcgcg gtgaacctgg cgaagcacgc ctcggtgccg
     4501 gtcgtgaacg gcctgaccga ctggctgcat ccctgccaga tcctcgccga catgctcacg
     4561 atgatcgagc acgggctgga tctggacggc gccgtcgtgg cctacgtcgg cgacggcaac
     4621 aacgtggcga actcctggat cgaggcggcg ggactctacg ggctgaccct gcggatcgcc
     4681 tgcccggagg gctacgaccc cgacatggcg ctggtggcgc aggtgcggaa cacggggaag
     4741 gggagggtgg agatcgtgcg cacgccggac gaggccgccg acggcgcgga cgttctctac
     4801 accgacgtct gggccagcat ggggcaggag gccgagcgcg agaagcggct cccggtgttc
     4861 cgaccctacc agatcaacgg cgcgctcctc gaccgcgcga agccgaacgc gctggtgctc
     4921 cactgtctcc ccgcccaccg cggcgaggag atcaccgacg aggtgatgga agggccgaac
     4981 tcgggcgtct tcccgcaggc ggagaaccga ctccacgtgc agaaggcggt gctctacgag
     5041 ctgctcgtcg ccggggccac gggacggagg agcggcgctg ggcgcgcggc tccggcgcgg
     5101 aagcccgcgg ctcgcaagaa agcggcaggg aaatcggccg gccggaagcc cgcggcacgg
     5161 aagccggccg cccggaagcc cacggctcgg aggaaggcgg cacggaagcg cccgcgtcgg
     5221 tgatcgacgg gcgcggccgc gggttgttga gggcgctcct tctcggcgcc ctcctcgccg
     5281 cgatcggctg cgccaagtcg gtgtttcccc cgggcgggcc gctcgacgtc acgccccccc
     5341 gcgtgatcgc gacgacccca gccgattcca gcgtgggcgt cgaccggaac accggggtcg
     5401 acttcctctt ctcggaggac atggaccacg gcagcgtgcg cgacgccacg cgcatccggc
     5461 cttccgtgga gcggccgcag ttccgctgga gcggccgcca tttccgcgtc ttctggggcg
     5521 agacgctccg ggtgaacaca acctaccacg tcgtcctccg cggatcggtc cgtgacgcgc
     5581 acggtgtcct catgggcgct cccgtctcga tccacttctc gaccggtgac tcgctcgcgc
     5641 cggggaagat cacgggggtc ctgcgcgcgc gcacgctggg gcgctccggc gtgccgatcc
     5701 tcgtcttctc cgactcgctc ggcgcgcggc cggacaccgg cgtcacgttc gagccgctct
     5761 acgaggcggc caccgacacc gccggggtct actccatccc gggcatgatc ctggggccgg
     5821 agtaccgcgt cttcgccttc tatgaccgga acgggaacaa cacgtacgac gaagggcagg
     5881 acgtgctcgc gccgcaccag gagccgatcc gattgacacc cgaacgaccg gtcgcggata
     5941 gtatcaacat cgtcgcggtg gacccgcgcg cgctcgcggt cctcaccggg acgatcgtcg
     6001 cctccgactc gacggcccgc tatcgggtcg aggcccgcgg cgcggccgac tccacggtga
     6061 cggcgccgcc ggtccagcgc atcggcccgg gcgcgttcac gatgcgggtg cccgcgggca
     6121 cctggaggct ccgcgcgttc cgcctcgagg ggctgagcgg gtcgccgccg cgcctggagc
     6181 tgcgctggcc ggaggcggtg gtgctccggc ccgaggagga acgcggcggg ttcacgttcg
     6241 acttccggcc ggccgagggc cccggcgctc cgccgccccc gccgaaacga tagacgtctc
     6301 cgcgaggagg aaggagaagg ccatgagcga gcgaaccggg atcttcgagg gagtgggcgt
     6361 ggcgctggtc acgccgttcc gcggcggggc ggtggatcgc gaggcgctgg ccgcgctggc
     6421 ggcgagcctg gtcgcgcgcg gcgtgcgcgc gctctacccc tgcggctgca cgggcgaggc
     6481 gacctcgctt acgcgcgagg agcgcgcggc cgtgatccgg atcgccgtcg aggcggcgcg
     6541 cggcaaggcc gccgtcgtgg ccgggaccgg cactgccgtc accgacgaga cgatcgaatt
     6601 ctcgcgcgag gcgatccgcc tgggcgtgga cgccgtcatg gtgatcacgc cctactccat
     6661 ccggcccacg caggaggggc tgatcgccca ctaccgcgcg gtggccgacg cgatcgaccg
     6721 cccgatggtc ctgtacaacg ttccgacgcg caccggcgcc aacatcctcc ccgagacggt
     6781 cgcgcgcctg ggcgagcatc cgcgcatcgc ggccatcaag gaagccagcg gctcgctgga
     6841 ccaggcgagc cagatccgag gccgctccga catcaccatc ctcgcggggg acgacgcgct
     6901 ctacctgccg ctgctctcga ttggagcgaa aggagtcgtt tcggtcgccg ggcacctgac
     6961 gccggaggat ctcgtgctga tggggagcca ttatcgcgcg ggacgcgtcg cggaggccga
     7021 ggcgatccac cggcgcctct acccgctctt caaggcgctc ttcatcgaga ccagcccggc
     7081 tccggtcaag tacgccctct ccgccctcgg atcgatcgcc ccggacctcc ggctcccgct
     7141 ggtcggcctg aagccggaga cgatccgagc cgtggacgcg gcgctggacg gcctgggcct
     7201 tcgcgcgccg gcgcgcgccg cgcactagcg gcgtgggcgg ctccgcggcc gtcgttccca
     7261 tcctcctctt cggcgcgacc ggccgtatgg gccgggcgat ccgcgcctcc atcgcggcgt
     7321 cggcggcttc ggagcgggga acgttctcgc tccggggatg cgtcgcgcgc tcgcacgacg
     7381 gcgagcccca tcccgaaggg tgtgtctggt tcggccccga cgccctggcc aagcccgagg
     7441 gcctggcgtc gcttccgccc gatctggtcg tgatcgacgt gagcctcgcc gccggcaccg
     7501 aggcgctcct cggcgccctc gaggcgtcgc cgcgggcgct ggtggccgcg accacggcgc
     7561 tgtcggccgc gaccgaggaa cggatcgccc ggctcgccgg gcgggccccg atccttcgcg
     7621 cgcgcaacct gagcccgggg atcgccgttc tcgcggccct gctccgcgcc cttccgccgg
     7681 gagcgcgcgg cctctacgat gtcgacgtcc tggagcacca ccacgccgcg aagaaggacg
     7741 cgccgagcgg cacggcgatc gcgctcggcg agctgctcgg cggcgtgggc accgagcggc
     7801 gccccggcgg cgtggcgatg cattcgatcc gcgggggcag cgcccccggc acgcacgaga
     7861 tcgtcctgag cggcgaaggg gaggtgctca cgctggggca catggtgcac gaccgcgccg
     7921 tcttcgcgcg gggcgcgctc cgcgcggcgc gcttccttca cggaaggaag cccggcctct
     7981 actcgttcga agacgcgttg accgaagcct aggaggaatc gaacccgcat gcgcttttcc
     8041 caggcgttca tcccgaccct gaaggaagac ccggccgacg ccgagctggt gagccaccgt
     8101 ctcatgatcc gcgcgggcat gctgcgcaag cacgcggcgg ggatctacag cctgctcccg
     8161 ctcggctggc gcaccgttcg caaggtatcg cagatcattc gcgaggagtt gaatcgggag
     8221 ggagcgcagg agatctcgct cccgatcctc atgcccagcg agctgtggat ggaatcggga
     8281 cgatggcaga agcacgggaa ggagctcttc cggcagaagg accggcacga tcgcgactat
     8341 gcgctcgggc ccaccaatga agaggcagtg gtggatctcg tccggaacga ggtgcgctcc
     8401 taccgggacc ttcccctcaa cctgtatcag atggggatca agttccggga cgaggtgcgg
     8461 ccgcggtttg gcctgatgcg cgcgcgggaa ttcctcatga aagacggcta ctccttccat
     8521 gccgacgaat cctctctcga ggaaggctat gaggcaatgc gtcgcgccta tacggcgatc
     8581 ttccggcgct gcgggctctc ctttacgatg gtcgaggccg actcgggcgc gatcggcggc
     8641 gatgtgaacc atgagttcat ggtcaccgcg gaatcagggg agtcggaggt cttctcgtcg
     8701 gcctgcggct acgctgcgtc gtcagagcga gccaagttcg cgctcccacc ggcgccccac
     8761 gagccggaag cggcgctcgc cgagcgggag acgcccggga agaagagcgt cgaggaggtc
     8821 gccgcgttcc tcggcgtgac cagcgaccgc ctgctgaaga ccctggtctt ctgggccggc
     8881 ggcgatccga tcctcgccgt cgtgccgggg gatcgcgagc tgaacgaggc gaagctgagc
     8941 cgctacctgg gcggcgcgcc ggtgcgcctg gccgccgccg ccgagatcga gcagctcacc
     9001 ggggggccga tgggcttcac cggcccggtg gggctggaga cccgcatccg cacgatctgg
     9061 gatccctcgg tgcaggaggg gaagaactgg gtcgcgggcg ggaacaagaa ggacgtccac
     9121 ctcgtgaacg tccgcctggg ccgggacgcc gccgccaagg agcgcgccga cgtggccacc
     9181 gcgcgccggg gcgaccgctg tccgcgctgc ggcgacgagc tggtcgtgtc gcgcggcatc
     9241 gaggtggggc acatcttcaa gctcggcacc cggtattccg agccgatgaa ggcgcgcttc
     9301 ctggacgcgg cgggggcgga gaagacgatc gtcatgggaa cctacgggat cggcatcacg
     9361 cgcaccgtcg cggccgtcat cgagcagtcg cacgacgaga acgggatcgt ctggccgatg
     9421 ccgctcgcgc ccttccacgt ccacgtgctc gccgtgaacc agaagcacga agcctcccgc
     9481 gagacggcgg agaagatcgc cgccgatctc gaggcgcgcg gcgtcgaggt gctgttggac
     9541 gatcgcgacg agcgcccggg cgccaagttc aaggacgccg atctcctcgg gatgccgctg
     9601 cgcgtgacgg tgggcgagcg gggcttggcg gaaggtgtgg tcgaggtgcg ggatcggaag
     9661 gccggcgccg tggagaaggt gccggtggca gaagcttcag ggattgttgc caggagagtc
     9721 caggaagccc tgagatcgtc cgaatagact ggacgcaccc gcaacttcag cgtcaggtcg
     9781 aacgtctcca ttcattccgc cgtcctggac actcggtcga tgacggtctt caatgccgtg
     9841 agaaactcct cttccgtgac tcgagtgccg ggatcctgat gaggatcgag ggacagaacg
     9901 cgaacacgct ctcttgcttt gcgtgcttcg gggtcggaca gcccccgaag gcgaccctcg
     9961 agttccagat ccgacgcccg cagagcctcc tcgagcgcta ctcgtggggg tgggctgggc
    10021 ggatgttgcg tcccatcggc gtcgatgcgc caggaccccg tgcctggctt aggggggcgg
    10081 gagagaaaga ccatgtggac tccgtcgaag gcgagcatgg cgctgcctcg tcgatgcacg
    10141 acgggccgcg cggaacccga gtccggcaga agcgcgaaag ggttgaggcc gcatagcatt
    10201 ggcgaatagt ctctcggacg cccccagtac ccgctgtccg caaacacgat gtatcgggtc
    10261 ccaacctcaa atggaacgct gtgctcggac cagatcgtca tcccgtcaac ggtgccacca
    10321 tcctggatca atacgaggct gtcgttggat gccttgccct tgaggcatcg aaggtggccg
    10381 aagcaatacc tggtaacgat cgtttggggt gttcgcacgc aagtggcggc tcgcagagtt
    10441 cccacgaaga tcagggtggc attctcgatc tgccattcga acgatgtggc ggcgcacagg
    10501 gcgtggagcg gacgaggatg aattggagac agcaggagcg ccgttacgaa tgcgatgcgc
    10561 agtacgcgca cggtcggtta ccaaccggct ggctcatcgg aggggtggat gccacgatcc
    10621 taatacaccg ctgctcggcc tggcgagagg gctccaacct gccgggccgc accggaccca
    10681 tccgaccgtg aggacggatt acctccgatt acttggcagc cttggtacgg ttcgcggcct
    10741 tcgcgaggcg cgacttccgg cggtttgcga ccgccttctt gatgacgccc ttcttcacgg
    10801 cccggtccag ctccgaaacc gtcgccttca taacgtccgt attcgcaggc tgatcggcca
    10861 ttctcttcag aagggtcttc aactcggagc ggacggcgat gttcttggtg cggtcgcgct
    10921 tggtggtgcg catgcgcttc acggctgact tgtggtgcgg cattcgtctc ctccggacag
    10981 gcgaagcatc gaaacaggaa tgagcccagg agactagcgg tccggcccct gcctgtcaat
    11041 cgcaaccgct tgagtggtat actgctgcgg taaccgcacc tatggcgcca tccacgctcg
    11101 aatcccagat cgccgccctt cccgcgcagc ccggggtgta catcttccgg ggcgcgaaag
    11161 gggagctgct ctacgtcggc aaggccaagc ggctggatca ccgcgtccgc tcccacttcg
    11221 ccaaccccga gcagatcggt ccgaagcagg tgggactgat ccgccgcgtg accgacgtgg
    11281 agtacatcgc ggccgaatcg gagatcgacg cgctcctcct tgaggcgacg ctcgtccgcg
    11341 agcggcagcc gccgtacaac atccggctca aggacgacaa gcgctacccc tacgtccgcg
    11401 tctcctggca ggagcccttc ccgcgcgtga cgctggtgcg gcgcgtcgag aaggacgggg
    11461 cccgctactt cggaccctac accgaggtga gcgcgctccg ctcgctcctg cgccacatcc
    11521 agacgatctt cccgatccgg acctgccgcg acatcg
//
LOCUS       DASSLZ010000016         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1401637, whole genome shotgun sequence.
ACCESSION   DASSLZ010000016 DASSLZ010000000
VERSION     DASSLZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401637"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VFM00_00565"
     CDS             complement(<1..1260)
                     /locus_tag="VFM00_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496770.1"
                     /translation="MDHDGQPIRRPPDWERSFYADLVHEGLVQPLSHAFDIPDKLLGL
                     AGAVGARTRRQAVNVNAFDEVPNSTWFTNRNHLRAIPPDLLTQGPDSTLLPKKPWTVT
                     HKKHGGASVGFQIRDAAGRKWLVKLDWKGLPGLSSGADVVARTILHAAGYNVPHNEPV
                     RFSREDLRISEELARGDGKERFTSADLDSALARGAVLPDGEHLATASLFLSGHPLGAG
                     SMSRKRRGDGNDWYAHPNRRELRGFYVLASWIGYWDTKNANLLDLFDSTGVEGGHVEH
                     YLLDIGSSLGADADGLKRPESGYENAVDYAWMGRRLVTLGFAVEPWRRAHQDTGIPSL
                     GRFESTVFDPARFKPEVPNPAFRAMTDRDAYWGAKIVASFSDAQIRAAVAAAHYGDPR
                     TPELVTRLLIERRDKIARYWFGRVAPLD"
     gene            1473..2150
                     /locus_tag="VFM00_00570"
     CDS             1473..2150
                     /locus_tag="VFM00_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HET7496771.1"
                     /translation="MIAGVDWFEGSWLAVVEDAAGEITVQRVSAFTDLLRRPDLETLV
                     IDVPIGLPDRGARVCDLQARKAIRPRGSSVFPAPIRPVLGARSQAEASQIRYAIEGKR
                     CSAQAYAIYPIVADVDRQMSPEAQRRIREGHPELSFAGMNGGAGLQWPKRSDEGRRER
                     IHHLAPYFETLSAHVDSIEPRRAVIDLLDAFAMLWTARRVRSGRAVSLPDLPEYDARG
                     LCMEIVA"
BASE COUNT          352 a          748 c          787 g          357 t
ORIGIN      
        1 gtcgagcgga gccacccggc cgaaccagta gcgcgcgatc ttgtcgcggc gctcgatcaa
       61 caggcgcgtc acgagctccg gcgtccgtgg atcaccgtaa tgcgcggcag ccacggccgc
      121 gcggatctga gcgtcggaga acgaggccac gatcttcgcc ccccagtagg cgtcccggtc
      181 ggtcatggct cggaacgcgg ggttcggcac ctcgggcttg aagcgcgcgg ggtcgaagac
      241 cgtcgattcg aatctcccca gggaggggat gccggtgtcc tgatgcgcgc gtcgccacgg
      301 ctcgaccgcg aagcccaggg ttaccaggcg gcgccccatc caggcgtagt ccaccgcgtt
      361 ctcgtatccc gactcggggc gcttcaggcc gtcggcgtcg gcgccgagcg aagagccaat
      421 gtcgagaaga tagtgctcga cgtggccgcc ctcgactccc gtgctgtcga acaggtcgag
      481 gaggttcgcg ttcttggtgt cccagtagcc gatccaggag gccaggacgt agaagccgcg
      541 cagctcacgg cggttcggat gcgcgtacca gtcgtttcca tccccgcgac gcttccggct
      601 catgctcccc gctcccagcg gatggccgga aaggaagagg ctcgccgttg ccaggtgctc
      661 cccatcgggc agcacggcgc cccgcgcgag ggcgctgtcg agatcggcgg acgtgaaccg
      721 ctccttgccg tcgcctcggg ccagctcttc ggaaatccgg agatcctcgc gggagaatcg
      781 caccggctcg ttatgcggca cgttgtagcc cgccgcatgc aggatcgtgc gcgccaccac
      841 gtcggcgccg gagctcaagc cgggcagccc cttccagtcc agcttcacga gccacttccg
      901 tccggcggcg tccctgatct ggaatccgac gctcgctcct ccgtgcttct tgtgggtgac
      961 cgtccacggc ttcttcggaa gcagtgtgga gtcgggaccc tgtgtcagca ggtccggggg
     1021 gatcgcgcgc aggtggtttc gattggtgaa ccaggtggaa ttgggcactt cgtcgaaggc
     1081 gttcacgttg accgcctggc ggcgcgtccg ggcgccgacc gcgccagcga ggcccaggag
     1141 cttgtcgggg atgtcgaacg cgtgggagag cggctggacc agcccctcgt gaaccagatc
     1201 cgcgtagaac gaccgctccc agtcaggagg gcggcggatg ggctggccat catgatccac
     1261 ccaggtactg tccgtgacgg gctgtgcggc ggcgcacgga gcggcaggcg tcatgagccc
     1321 gaggatgagc gcgatgcagg tggatccggc cccgcacctg agcctgcgcg gaaacatcct
     1381 gaaagggtac accacttttg cggggagcct ggaaccactc gagtcttgcg cacgtagttc
     1441 tctcgggacg agggacaacc cgggagggcg ccatgatcgc cggtgtggac tggttcgaag
     1501 gctcctggct cgccgtggtg gaggacgccg cgggggagat cacggtacag cgggtatcgg
     1561 cgttcaccga tctccttcgc cggcccgatc tcgagacgct cgtcatcgac gtcccgatcg
     1621 ggctgccgga tcggggagcc cgcgtgtgcg accttcaagc gcgaaaggcc attcggcccc
     1681 gcggcagctc cgtcttcccc gccccgatac gaccggttct gggcgccagg agccaggccg
     1741 aggcgagcca gatccgctac gcgatcgagg ggaagcggtg ctcggcgcag gcctatgcca
     1801 tctaccccat cgtggccgac gtggaccggc agatgtctcc cgaggcgcag cggcgcattc
     1861 gcgaagggca tccggagctg tcgttcgccg ggatgaacgg aggcgccgga ctccagtggc
     1921 ccaagcgcag cgacgaggga cggcgtgagc gcatccacca cctcgccccc tacttcgaga
     1981 ccctttctgc gcacgtcgac tcgatcgagc cgcggcgcgc cgtgatcgac ctgctcgacg
     2041 cgttcgcgat gctctggacc gcccgccgtg tacgctccgg gcgtgcggtc tcgcttcccg
     2101 atctgccgga atacgacgct cgtggcctgt gcatggaaat cgtggcgtga aacaccgcga
     2161 gggcgttcag gggtggagcg cgcgggcgat cgcccgcgcg aagcgcgccg gcgcttccgg
     2221 atggcgcgac aggaacatca aggg
//
LOCUS       DASSLZ010000017         5363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1528973, whole genome shotgun sequence.
ACCESSION   DASSLZ010000017 DASSLZ010000000
VERSION     DASSLZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528973"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VFM00_00575"
     CDS             <1..915
                     /locus_tag="VFM00_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HET7496772.1"
                     /translation="ESFDEPFANASAVPTYYCARVAREHGVTALYAGDGGDELFAGNQ
                     RYADGRVFEPYDRVPRWIRDPLLTPAIAAAGKIVPHPLFVKAGKYVRRASLPPAERIV
                     SYDFWNVVDPAAFATPDLMAAAAGWRPAAMVIRHHGQARATTALDRHLYLDLKITISD
                     NDVPKVARMCERAGVAVRFPFLDERVVDVATRVPARIKMRGGELRTFFKDAYKDLLPA
                     ETRAKTKHGFGLPISGWLKSDPGLRAMMRDLVLSPRSVERGYFRREALEDVVRRHEED
                     ATPYYGTVLWNLMVIELWHRRVLDGRLP"
     gene            965..2095
                     /locus_tag="VFM00_00580"
     CDS             965..2095
                     /locus_tag="VFM00_00580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7496773.1"
                     /translation="MSAPDRFEVVEPARAFGELAPAWEDLLRSSAADSPFLTPVWLEA
                     WWDAYGAGREPLLVFARRGDAIVGIFPLQVAIERWRGRLPLRTLRLFGDGTHDSDYLD
                     FIVPRGEEEAVLPAFWSWLRRAGSGMRYEVAQWNEIPDASPAGAVIRRLAERDGSLLE
                     EHRVGCVVTALPPSYDAYVASLKPRMRTKVRSLRRVLSEQHGALFQRADEATLGPTLE
                     SLFQLHERRWTLRGESGVFAAPEKRAFYHAMGRALLTRGWLDLTTLIAGGTPVAHQCC
                     IRYRETAFLLQEGYDPDWEDRGVGNALRAMTIEAMIQDGLHTYDFLAGVTSHKLSWGG
                     TVKESLRLTLRGSGARAAIASGLSLLSAARARLRAAFSRSGR"
     gene            2092..3201
                     /locus_tag="VFM00_00585"
     CDS             2092..3201
                     /locus_tag="VFM00_00585"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7496774.1"
                     /translation="MSAAKDAAASLFQAAGGEPLLRAAGNRWMLVSGDGGARLRRRAS
                     PPFLILIYHRVHPNPGPFMIDPVPPDRFERQMRHLARAYRPLPLAELLERSRAGAVPE
                     GAVAVTFDDGYADNAEYAAPILARHGIPATVFLVTGCIGTGRIPWHDEVLLAFAAARG
                     GSIKIPGQPADAPPLPLETEAERHRAAFLALGALKPMHEAERLAGVRAIQETLGPGDP
                     GAAASLMLDWGRVRAMRGSGIAFGSHTETHPILSRVAPERAREEVMRSKRTIEKELGE
                     EAAFFAYPNGRVEDYDEGAVQALRDAGYRGALTTSFGPNEAGEDPFRWRRAAAWSADP
                     RRFALQLAWYRFRGPESPAGALGPGRALAGAGGGR"
     gene            3204..4505
                     /locus_tag="VFM00_00590"
     CDS             3204..4505
                     /locus_tag="VFM00_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HET7496775.1"
                     /translation="MRDILIALLVFGSAPVALFRPDIGILAYIWIGLMNPHRLSWRLE
                     YAPVGLVVAIATLLGTVIRGEIRRIPMKSTTILLMAWMLYTTLTTMTAISGRDAWIEW
                     NRFSRIIFMCFVAMMLLQERKRLERYVWVAMASIAFFSVKGGLFSIATRGAYRVWGPM
                     GSFIEGNNELALAEIMILPLMLYFARQVKKAWQRWAYYGSIVLTIFSILFSYSRGAFV
                     AFAGVAGILLVRSRYRFQGLLMVLVLGAALVSFVPAEWKERMTSIESYQTDQSAQGRI
                     NAWHFAFNIAKTHLFGGGFRTFTKELFMVYAPNPTDVHDAHSIYFEVMAEQGFPGLMI
                     FLGILVTALWKLERMRKRWPRDSERRWARDLAEMLQFSLLGYMFGGAFLGLAYFDLPY
                     YIVVSTILLESIAERQPAAVPVPSPSETARSRALGRAPAAG"
     gene            4509..>5363
                     /locus_tag="VFM00_00595"
     CDS             4509..>5363
                     /locus_tag="VFM00_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7496776.1"
                     /translation="MRLVVDHRTQGQGVEGVHLLGMAGAFERAGAEVAIVSPPGVEVG
                     RPGAAPRKAPGLRGLWWMLAEHVPETLFELMEMVYNVPAYFRLRRALLTPPADALYER
                     YAFFHVAGALAASAAGVPLILEVNYTAATPLYRSRSRLLRPLARAAERFVFRRARLIV
                     AVSSVLRDAIVAGGIPADRVLTLPNAADPDRFRPEISGEAVRARYGLRGARVVGFTGA
                     FFPWHGVGFLLDALAALLRDMPEAAALLVGDGPERPILEERVRREGLEARVRFAGWIG
                     HDGLPEHVA"
BASE COUNT          683 a         1943 c         1886 g          851 t
ORIGIN      
        1 gaatcgttcg acgagccgtt cgcgaacgcg tcggccgtgc ccacgtacta ctgcgccagg
       61 gtggcccgtg agcacggcgt gaccgcgctc tacgcggggg acggcgggga cgagctcttc
      121 gcgggcaatc agcggtacgc cgacggccgc gtcttcgagc cctacgaccg ggtgccgcgc
      181 tggatccgcg accccctcct cacgccggcg atcgcggcgg cgggcaagat cgtgccgcac
      241 ccgctcttcg tgaaggcggg gaagtacgtc cggcgggcgt cccttccccc cgcggagcgc
      301 atcgtttcct atgatttctg gaacgtcgtg gatcccgccg ccttcgccac gccggatctc
      361 atggccgccg cggccggctg gcgcccggcc gcgatggtga tccggcatca cggccaggcg
      421 cgcgccacga ccgccctcga ccgccatctc tatctcgacc tcaagatcac gatcagcgac
      481 aacgacgtcc ccaaggtggc gcgcatgtgc gaacgcgccg gagtcgccgt ccggttcccg
      541 ttcctcgacg agcgcgtggt ggacgtggcg acgcgcgtgc ccgcgcggat caagatgcgc
      601 gggggagagc tccgcacgtt cttcaaggat gcctacaagg atctcctccc cgccgagacg
      661 cgcgcgaaga ccaagcacgg cttcggcctt ccgatctccg gctggctcaa gagcgatccc
      721 ggcctccgtg cgatgatgcg cgacctggtg ctctccccgc gcagcgtgga gcgcggctac
      781 ttccggcgcg aagccctcga ggacgtcgtg cgccgccacg aggaggacgc gacgccgtac
      841 tacggaacgg ttctctggaa tctcatggtg atcgagctgt ggcatcgccg ggtgctcgac
      901 gggcggttgc cctgagcggc gctccggtgc gggaggcgca cgacccggcg tccgggaagc
      961 gccggtgagc gcgcccgacc gcttcgaggt ggtggagccc gcgcgcgcct tcggcgagct
     1021 ggccccggcg tgggaggacc tcctgcgctc gagcgcggcc gactcgccct tcctcacgcc
     1081 ggtatggctc gaggcctggt gggacgcgta cggcgccggg cgcgagcctc ttctcgtctt
     1141 cgcccggcgc ggcgacgcga tcgtcggcat cttcccgctc caggtcgcga tcgagcggtg
     1201 gcgcggccgc ctcccgctcc ggacgctccg gctgttcggc gacgggacgc acgattcgga
     1261 ctatctggat ttcatcgtgc cgcgcggcga ggaggaggcc gtccttccgg cgttctggtc
     1321 atggctccgg cgtgccggct ccgggatgcg ttacgaggtg gcgcagtgga acgagatccc
     1381 ggacgcgtcg cccgcgggcg ccgtcatccg ccggctcgcc gagcgggacg gatccctcct
     1441 ggaagagcat cgcgtcggat gcgtcgtcac cgcgctgccg ccgtcctacg acgcctacgt
     1501 cgcgtcgctc aagccgcgga tgcggaccaa ggtccgctcg ctgcggcggg tgctctccga
     1561 acagcacggc gccctgttcc agcgcgcgga cgaagcgacc ctggggccga ccctcgaatc
     1621 gctcttccag ctgcacgaac ggcgctggac gctgcggggg gagtccggcg tcttcgccgc
     1681 tccggagaag cgcgccttct accacgccat gggacgcgcc ctgttgacgc gcggttggct
     1741 ggacctgacg accttgatcg ccggcgggac acccgtggcg caccagtgct gcatccgcta
     1801 tcgcgagacg gcgttcctcc tccaggaggg gtacgacccc gattgggagg accgcggcgt
     1861 gggcaacgcg ctccgcgcga tgacgatcga ggccatgatc caggacggcc tccacaccta
     1921 cgacttcctc gcgggcgtca ccagccacaa gctctcctgg gggggaaccg tgaaggagag
     1981 cctccggctc acccttcggg gaagcggggc gcgcgcggcg atcgcgtcgg ggctctcgct
     2041 cctctccgcc gcgcgcgcgc ggcttcgcgc cgccttctcc cggagcgggc ggtgagcgcc
     2101 gcgaaagacg ccgcggcgtc gctcttccag gccgcgggag gggagccgct gctccgcgcg
     2161 gcggggaacc gctggatgct cgtctccggc gatggaggcg cgcgcctgcg ccggcgcgcg
     2221 tcgccgccgt tcctgatcct gatctaccac cgcgtccacc ccaaccccgg accgttcatg
     2281 atcgatccgg tgccgccgga tcgcttcgag cgccagatgc gccacctggc gcgcgcctac
     2341 cggccgcttc cgctcgccga gctcttggag cggagccgcg cgggagccgt ccccgagggg
     2401 gcggtcgcgg tcacgttcga cgacggctat gccgacaacg ccgagtacgc ggcgccgatc
     2461 ctggcgcgcc acgggattcc cgcgacggtc ttcctcgtga ccgggtgcat cgggaccggg
     2521 aggatcccct ggcacgacga ggtgctcctc gcgttcgccg cggcgcgcgg cggctcgatc
     2581 aagatcccgg ggcagcccgc ggacgctccg ccgcttccgc tcgaaaccga ggcggagcgg
     2641 catcgcgcgg ccttcctcgc gctgggcgcg ctcaagccga tgcatgaggc cgagcgcctc
     2701 gccggcgtgc gcgcgatcca ggaaacgctg ggtcccgggg atcccggcgc ggccgcgtcg
     2761 ctgatgctcg actggggccg cgtgcgcgcc atgcggggat cggggatcgc cttcggctcc
     2821 cacacggaga cccatccgat cctgagccgc gtggcccccg agcgggcgcg cgaagaggtg
     2881 atgcgctcga aacggacgat cgagaaggag ctgggggagg aggccgcctt cttcgcctat
     2941 cccaacggcc gcgtcgagga ctacgacgag ggcgcggtcc aggcgctccg cgacgcgggg
     3001 taccggggtg ccctcacgac gtcgttcgga ccgaacgagg cgggggagga tccgttccgc
     3061 tggcggcggg ccgccgcgtg gtcggccgat ccgcggcgct tcgcgctgca gctggcgtgg
     3121 taccggttcc gcggccccga gtcgccggcc ggggcgcttg ggcccggccg cgccctggcc
     3181 ggggccggag gaggccgcta gcgatgcgcg acatcctgat cgctcttctc gtgttcggca
     3241 gcgctcccgt cgcgctcttc cgccccgaca tcggaatcct ggcctacatc tggatcgggc
     3301 tcatgaaccc ccaccgactc tcgtggcggc tggagtacgc gcccgtcggg ctggtcgtcg
     3361 ccatcgccac gctgctcggc acggtgatcc gcggggagat ccggcggatc ccgatgaagt
     3421 ccacgacgat tctcctcatg gcctggatgc tctacacgac cctgacgacg atgacggcca
     3481 tctccggccg cgacgcctgg atcgagtgga atcgcttctc gcggatcatc ttcatgtgtt
     3541 tcgtggcgat gatgctgctc caggagcgga aacggctcga gcgctacgtc tgggtggcga
     3601 tggcctcgat cgccttcttc agcgtgaaag gggggctctt ctcgatcgcc acccgcggcg
     3661 cctatcgcgt gtgggggccg atggggtcct tcatcgaggg aaacaacgag ctcgccctgg
     3721 ccgagatcat gatccttccg ctcatgctct acttcgcgcg ccaggtgaag aaggcctggc
     3781 agcgatgggc ctactacggc tcgatcgtgc tgacgatctt ctcgatcctc ttctcctatt
     3841 cgcgcggcgc cttcgtggcg ttcgccggcg tcgcgggcat cctcctcgtc cgctcccgct
     3901 accggttcca gggcctcctc atggtgctcg tgctcggagc ggcgctggtt tccttcgtgc
     3961 cggccgaatg gaaggagcgg atgacctcga tcgagtcgta tcagaccgac cagtccgccc
     4021 aggggcggat caacgcctgg cacttcgcct tcaacatcgc gaagacccat ctcttcggag
     4081 gcggtttccg caccttcacg aaagagctct tcatggtgta cgccccgaac ccgaccgacg
     4141 tccacgacgc gcacagcatc tacttcgagg tgatggccga gcagggattc cccgggctca
     4201 tgatcttcct cggcatcctc gtcacggccc tctggaagct ggagcggatg cgcaagcgct
     4261 ggcccaggga ttccgaacgg cggtgggccc gcgacctggc cgagatgctg caattctcgc
     4321 tgctgggcta catgttcggc ggcgccttcc tgggactcgc ctacttcgat ctgccgtact
     4381 acatcgtggt ctcgacgatc ctcctcgagt ccatcgccga gcgccagccg gccgccgtcc
     4441 cggtcccgtc gccgtcggag acggcgcggt cccgcgcgct cggccgcgcg cccgcggcgg
     4501 gctagcgcat gcggctggtc gtcgaccacc ggacgcaggg gcagggcgtg gagggggtgc
     4561 acctcctcgg catggcgggc gcgttcgagc gcgcgggcgc cgaggtcgcg atcgtctcgc
     4621 cgcccggcgt ggaggtggga cgccccggcg ccgcgccgcg caaggcgccc ggcctccggg
     4681 gtctctggtg gatgctcgcc gagcacgttc ccgagacgct cttcgagctg atggagatgg
     4741 tctacaacgt ccccgcctac ttccgcctcc ggcgcgcgct cttgacgccg ccggccgacg
     4801 ccctgtacga gcgctacgcc ttcttccacg tcgcgggcgc cctggcggcg agcgccgcgg
     4861 gcgtgccgct gatcctcgag gtgaactaca ccgcggcgac gccgctctac cgcagccgga
     4921 gccgcctgct tcgacccctc gcgcgcgccg ccgagcgctt cgtcttccgc cgcgcccggc
     4981 tcatcgtggc ggtatcctcg gtgctccggg acgccatcgt ggctggcggc atccccgccg
     5041 accgggtgtt gacgctgccc aacgccgccg atcccgaccg cttccgcccc gagatctccg
     5101 gagaggcggt gcgggcgcgg tatgggctgc gcggggcgcg cgtcgtgggc ttcaccgggg
     5161 cgttcttccc ctggcacggg gtgggattcc tgctcgacgc gctggcggcg ctcctgcgcg
     5221 acatgcccga ggcggcggcc ctgctggtag gggatggccc cgagcggccc atcctcgagg
     5281 agcgggtgcg gcgcgagggc ctggaggcgc gcgtgcgctt cgccggatgg atcggccacg
     5341 acggactccc cgagcacgtc gcc
//
LOCUS       DASSLZ010000018         4871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1654753, whole genome shotgun sequence.
ACCESSION   DASSLZ010000018 DASSLZ010000000
VERSION     DASSLZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654753"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFM00_00600"
     CDS             complement(<1..627)
                     /locus_tag="VFM00_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496777.1"
                     /translation="MRPILVVAAVAVALAAAFSAVPALARDDDPGELKLLLGFMGSDP
                     NLVNHGREQNWSPLVGLRIGANMDRPWSQFIDGVYSHVDSEPGDHSTVLEARVGMERN
                     FDLSGGGTWYLAGALGWADANLPTPRDDFGRPLASLGIGVRPASGPGARFHIEVREEW
                     WMGDEGLGGANVANTQVLVGIGFGLKDTREPMFERGSRTLILEGVNFIT"
     gene            776..1522
                     /locus_tag="VFM00_00605"
     CDS             776..1522
                     /locus_tag="VFM00_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7496778.1"
                     /translation="MSRARVLVVDDDSKILSSLRLYLEHAGYEVAAAADGQRALAVAR
                     SFRPDLVVLDVMLPKGDGLAVCRALRAESEVPILFLTARATEEDRLEGLDLGADDYVT
                     KPFSPRELVARVRAILRRARGEDEDEVPRRRSTAAEPQPPETLRVGSLRIDPARHEVM
                     VRGARVDLTPREFRLLHALARDPGRAMTRDELAERAFGPEYEGLARTVDAHVANLRRK
                     IEPDPASPVYVETVFGVGYRLRAGRRSGDA"
     gene            1534..2973
                     /locus_tag="VFM00_00610"
     CDS             1534..2973
                     /locus_tag="VFM00_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7496779.1"
                     /translation="MFRSLRARLLLLVAVVAGLSLAAAALLSRQAVRTEFLRLETSER
                     AARLGDAAAMLDTWLRRTGSLAGADSVLKRLRPPGARGSGGLVGAEFLLIAPDGTVLA
                     ASAPEYRAASVELRQDGGLGITVPRREGNLVRVRREVLVGGPRAEIHGPDGAVRATLY
                     RLPAIRPEAIPSPFIVSVNRWLLLAALISGALAILLTLALSRRILGPVEALTGAVRRM
                     ERGDLTARVPVRSSDEIAELSSAFNRMAASLEENEASRRRLLGDVAHELRSPLTNLRC
                     QIEAVEDGLATADAETMRSLHEETLLLGRLVDDIQDLALAEAGRLPLHRERVSPRAAL
                     EAAAAAFTPLAADRGVSLRAGGEGAPDVDADPARLKQVLRNLVANALAHTPEGGTVEL
                     DASSGGDGRVAFTVRDTGAGIAPEDLPHIFDRFYRADRARTRETGGSGLGLSIVKQLV
                     EAHGGSVAAESRPGAGTTVRFTLPQAPPS"
     gene            3057..>4871
                     /locus_tag="VFM00_00615"
     CDS             3057..>4871
                     /locus_tag="VFM00_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7496780.1"
                     /translation="MRRIALFLSLALFLAAAPAKKPAPAPKPAPESKPEAKALEFPNN
                     EAGRHAAAWFEAFNKGDEAMRKYIQAHVAPSALARRTVQDRMDIYHDMKDERGRVTPL
                     SIESFTESNVKAIARGERGGRFAITFRCEEEAPHGLLGLQVEDLPPEEGTPEGIEEGG
                     YSQAPVGPRMREAQVAAALRAYVDSLARADAFSGAILLARGNRVLERRAYGLASRRYG
                     TLNRPDTKFNLGSINKAFTKVAIQQLAAQGKLSLDDTIDKYVPDYPREAASKITIRML
                     LDHRGGVPDFFGPKFQNADRTQLRSISDWVQFIRYMPLRFEPGTREEYSNGGYILLGA
                     VIEKASGMDYYDYVRRNIYEPAGMKDTDSYQQDDPVRNVAEGYTKSAGRGTDSRWRDA
                     APMHPWRGSPAGGGYSTVDDLFRFAQALRSGTLGARGEAGMAIAGGSPGTNAMLMMQG
                     DWTLVVLSNLDPPAAERVGTKSGAWLRRAGASSGGANHRISARPLGAEVHSAREEKPA
                     STVLPAGGAVVPMAWAGHLASVEVTLNGQGPFRFAIDTGAAGCLRIDEAVARKLGLPV
                     VGEARVGDPSGKNMKTAEIVAVDSLAAGGARFAGLTATT"
BASE COUNT          692 a         1742 c         1755 g          682 t
ORIGIN      
        1 ggtgatgaag ttcacccctt ccaggatcag cgtgcgggac ccgcgctcga acatcggctc
       61 gcgcgtgtcc ttgagaccga agccgatgcc gacgagaacc tgggtgttgg cgacgttcgc
      121 tccaccgaga ccttcgtccc ccatccacca ctcctcgcgc acctcgatgt ggaagcgggc
      181 tccaggcccg gacgccggcc ggacgccgat gccgagcgac gcgagcgggc gtccgaagtc
      241 gtcgcggggc gtgggaaggt tcgcgtcggc ccagccgagc gctccggcca ggtaccaggt
      301 cccgccgccg ctcagatcga agttccgctc catgcccacc cgagcctcca gcaccgtgga
      361 gtggtcgccc ggctcggagt cgacgtggct gtagaccccg tcgatgaatt gggaccacgg
      421 gcgatccatg ttggcgccga tccgaaggcc caccagcgga gaccagttct gctcacggcc
      481 gtggttgacc aggttcggat cggaacccat gaatccgagg aggagcttca gctcgccggg
      541 atcgtcgtcc cgggcgaggg cgggcacggc ggaaaacgcc gccgccagcg cgacggccac
      601 ggcggccacg accaggatcg gtcgcatacg acctccatct cagggtctgt tgggaagccc
      661 ctcggggctt gggatggggc cagcctacac cggaccgcgc cgggcttcat gaaatcttca
      721 ccggttcgtg gcatgctcac ggggcgattc ccctaatttc ccttcggagc gccacttgag
      781 ccgagcccgc gtcctggtcg tggacgacga ttccaagatc ctgtcctccc ttcgcctcta
      841 cctggagcac gcgggatacg aggtggcggc ggcggcggac gggcagcggg ccctggccgt
      901 ggcccggtcc ttccgccccg atctggtcgt gctggacgtg atgctcccca agggagatgg
      961 gctcgcggtc tgccgcgccc tccgggcgga atcggaagtc ccgatcctct tcctgaccgc
     1021 gcgggcgacc gaggaggacc gcctggaggg gctggatctc ggcgccgacg actacgtcac
     1081 caaaccgttc agcccgcgcg agctggtcgc gcgcgtgcgc gccatcctcc ggcgggcgcg
     1141 gggcgaggac gaggacgagg tgccgcggcg gcgttccacc gccgcggagc cgcagccgcc
     1201 ggagaccctc cgggtcggct cgctccggat cgatcccgcg cgtcacgaag tgatggtgcg
     1261 cggcgcgcgc gtggacctca ccccgcgcga gttccggctg ctccacgccc tggcgcgcga
     1321 cccggggcgg gccatgaccc gcgacgagct cgccgagcgc gcgttcggcc ccgaatacga
     1381 gggcctcgcg cgcaccgtgg acgcgcacgt ggcgaacctc cggcgcaaga tcgagcccga
     1441 tcccgcgtcc cccgtgtacg tggagacggt gttcggggtc ggctatcggt tgcgcgcggg
     1501 acgccggagc ggcgatgcct agcgggacgc gccatgttcc gtagcctccg cgcgcgcctt
     1561 ctgctgctcg tcgccgtcgt ggcggggctc tccctcgccg cggccgcgct cctctcgagg
     1621 caggccgtgc gcacggagtt ccttcgcctg gagaccagcg agcgggccgc gcgcctcggc
     1681 gacgccgcgg cgatgctcga cacctggctg cgacggacgg gctccctcgc gggcgccgac
     1741 tccgtgctga aacggctgcg cccgccgggc gcgcgcggca gcggcggcct cgtgggcgcg
     1801 gagttcctgc tgatcgcccc cgacggcacc gtcctggccg cgagcgctcc cgagtaccgg
     1861 gccgcgagcg tggagcttcg ccaagacggc ggtctgggga tcacggtgcc gcggcgcgag
     1921 gggaacctcg tgcgggttcg gcgcgaggtc ctggtcggag gcccccgcgc ggagatccac
     1981 ggccccgacg gcgcggtccg cgcgacgttg tatcgcctgc ccgcgatccg gccggaagcg
     2041 attccctcgc cgttcatcgt ctcggtgaac cgctggctcc tcctcgcggc tctgatttcg
     2101 ggcgcgctcg cgatcctgct cacgctggcg ctctcacgcc gcatcctggg cccggtcgag
     2161 gccctgacgg gcgcggtgcg gcgcatggag cgcggcgatc tgacggcgcg cgtgccggtc
     2221 cgttcctcgg acgagatcgc ggagctgtcg agcgccttca atcgcatggc cgcttcgctc
     2281 gaggagaacg aggcgtcgcg gcggcgcctc ttgggcgacg tggcgcacga gctgcgctcg
     2341 ccgctcacga accttcgctg ccagatcgag gcggtcgagg acggcctggc gaccgcggac
     2401 gcggagacga tgcgctcgct gcacgaagag acgctcctcc ttggaaggct ggtggacgac
     2461 atccaggacc tggcgctggc cgaggcggga cggctcccgc tccaccgcga gcgggtgtcg
     2521 ccgcgcgcgg cgctggaggc cgcggccgcc gcgttcaccc ctctcgcggc cgaccgcggc
     2581 gtgtccctcc gcgcgggggg cgagggcgcc cccgacgtgg acgccgatcc cgcgcggctc
     2641 aagcaggtgc tccggaatct cgtcgcgaac gcgctcgcgc acacgccgga gggcggaacg
     2701 gtggagctcg acgccagctc cggcggcgac ggccgcgtgg cgttcacggt gcgggacacg
     2761 ggcgcgggca tcgcgcccga ggatctgccc cacatcttcg accgcttcta ccgcgcggac
     2821 cgggcccgca cgagggagac cgggggctcg gggctgggac tgtcgatcgt gaagcagctc
     2881 gtggaggcgc acggcggctc ggtcgcggcc gagagccggc cgggtgcggg aaccacggtc
     2941 cgcttcacgc ttccccaggc accgccttca tagctccttc atactctggg actatcgtcc
     3001 ctggcgtcgg ccgcgcgccg cggccggacc acgccgatcc aggaggaatg acgaacatgc
     3061 gacgcatcgc cctgtttctg tccctcgccc tgttccttgc cgccgcgccg gccaagaagc
     3121 ccgcgccggc gcctaagccc gcgccggagt cgaagccgga agccaaggcg ctcgaattcc
     3181 cgaacaacga agcgggccgc cacgcggccg cctggttcga agccttcaac aagggcgacg
     3241 aggcgatgcg gaagtacatc caggcgcacg tcgcgccctc cgcgctcgcg cggcgcacgg
     3301 tccaggaccg gatggacatt taccacgaca tgaaggacga gcgcgggcgg gtgacgccgc
     3361 tttcgatcga gtcgttcacc gaatccaacg tgaaggcgat cgcgcgcggc gagcggggcg
     3421 ggcggttcgc gatcacgttc cgctgcgagg aagaggcgcc gcacgggctc ctcggactcc
     3481 aagtggagga tctcccgcct gaggagggca ctcccgaggg gatcgaggag ggtggctatt
     3541 cccaggcgcc cgtgggcccc cggatgagag aggcacaggt ggcggcggcg ctccgggcgt
     3601 acgtcgattc cctcgcccgc gcggacgcct tttccggcgc gatcctcctc gccaggggaa
     3661 atcgcgtgct cgagcggagg gcctacggcc tggcctcacg ccggtacggg acgctcaacc
     3721 ggcccgacac caagttcaac ctcggctcca tcaacaaggc gttcaccaag gtcgcgatcc
     3781 agcagctcgc ggcccaggga aagctgagcc tggacgacac catcgacaag tacgtccccg
     3841 actatccgcg cgaggcggcg tccaagatca cgatccgcat gctgctggat cacaggggcg
     3901 gcgtccccga cttcttcgga cccaagttcc agaacgccga ccgcacccag cttcggagca
     3961 tctcggactg ggtgcagttc atccgctaca tgccgctccg gttcgagccg ggcacgcgcg
     4021 aggagtattc gaacggcggc tacatcctcc tgggagccgt catcgagaag gcctccggaa
     4081 tggactacta cgactacgtg cgccggaaca tctacgagcc cgcggggatg aaggacaccg
     4141 attcctacca gcaggacgac cccgtgcgga atgtcgccga ggggtacacg aagtcggcgg
     4201 gtcgcggcac cgacagccgc tggcgcgacg cggcgccgat gcacccgtgg cgcggaagcc
     4261 cggccggcgg cggctactcg accgtcgacg acctcttccg cttcgcgcag gcgcttcgct
     4321 cgggaacgct gggcgctcgc ggcgaggcgg gcatggcgat cgcgggggga tcgcccggga
     4381 cgaacgcgat gctgatgatg cagggagact ggaccttggt cgtgctctcg aacctggatc
     4441 cccccgcggc cgaacgggtg ggcacgaagt cgggcgcctg gctccggcgg gccggcgcct
     4501 cctcgggcgg cgccaaccat aggatctcgg cccgtcccct cggggccgag gtgcattcgg
     4561 cgcgcgagga gaagccggcc tcgacggtgc tccccgccgg gggcgccgtc gttcccatgg
     4621 cgtgggccgg gcacctggcc tccgtggagg tgacgctcaa cggccagggc ccgttccgct
     4681 tcgcgatcga cacgggggcg gcggggtgtc tccgcatcga cgaggcggtg gcgcgcaagc
     4741 tgggccttcc cgtcgtcggc gaggcgcgcg tgggcgatcc cagcggcaag aacatgaaga
     4801 ccgccgagat cgtcgcggtc gattcgctcg cggccggcgg cgcccgcttc gcggggctca
     4861 cggccacgac c
//
LOCUS       DASSLZ010000019         7226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1022027, whole genome shotgun sequence.
ACCESSION   DASSLZ010000019 DASSLZ010000000
VERSION     DASSLZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022027"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1160
                     /locus_tag="VFM00_00620"
     CDS             270..1160
                     /locus_tag="VFM00_00620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7496781.1"
                     /translation="MERKRASDYPQELLNLFDKYVHGGIDRRGFLIGAARFAVGGVTA
                     AMLLDELSPKFAEGQQVPPDDKRLTTTMLEYDSPQGSGTMKGYLARPAEAKDKKLPGV
                     LVIHENRGLNPHIEDIARRLALEGFMAFAPDALTPLGGYPGDEDKARELFQKLDQTKT
                     REDFVTAANLLRERPDCTGKIGAVGFCYGGGIVNFLATRMPELKAGVPFYGNQPAAAD
                     VPKIHAPLLIHYAETDERINAGWPAYEAALKESKKKYEVFFYPGTQHGFNNDTTPRYD
                     AAAAKLAWSRTVAFFRKHLT"
     gene            complement(1141..1407)
                     /locus_tag="VFM00_00625"
     CDS             complement(1141..1407)
                     /locus_tag="VFM00_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496782.1"
                     /translation="MRAAAGGRRVVVPSDESGDGFRELRRERGAIGRRSESDFRVDGQ
                     RREVFALFLRAAEEIPDLAHDACGQSDEVPRRKPIGGPLRSGAS"
     gene            1464..2282
                     /locus_tag="VFM00_00630"
     CDS             1464..2282
                     /locus_tag="VFM00_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET7496783.1"
                     /translation="MPTQKDPSGRRFIAVETEVPGTPEEVWEAIATGPGITSWFVPTQ
                     LEEREKGPIAMNFGPGMESKAEIQEWDPPRRFTAMNQEGMGPGAPPMATEWTIEAKAG
                     GTCRVRVVHSWFASTDDWDGQFESVEKGWPAFFRILKSYLAHFRGQPCTQVQIMAVAP
                     GPEEKAWSEITGPLGLAGANAGQSVTSSGDAPRLAAVVEHAGPKPHELMLRLEQPAPG
                     IAYLSTMPMGDQVGLWICLYLYGEGAAAVAAREEPVWQAWLARLFPAPVGQERS"
     gene            complement(2284..5922)
                     /locus_tag="VFM00_00635"
     CDS             complement(2284..5922)
                     /locus_tag="VFM00_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7496784.1"
                     /translation="MRHSTASPLRAVLVVGLLLLGLPSPGAAKEQETKAAILYDGSTS
                     GSTLLAPPKALLSGTLLKQSATTTSWFLYPNACAERALNTWSPRTSPVADSLNSYSAG
                     TTGPYTAQDQSLKETLWHVFDTGVPASQRPAPLEGTRSLWCGKYDPKAVQTVGYPNVT
                     YQILYVDTGAHSGSYTLTLLFQGSTEPGYDFLYLIGGGGAAVDPIGNSRPVLDNVIEN
                     GSNGASELLVTWTGTITPSTPGAASISTLGGPVSILGAEGGAPATVSTSITIAPGHRA
                     LYFVFTSEERMSSEDGLWPNGNGAELDLLATSDNGSIYSNQAAVGGTDGFGGDVIVGT
                     PGSPTVSARVPTAIGTLWHLAVGSSKPTTDFCSPAKGLGSDLFFEAGDFGTGMTDPGS
                     FASIVTCTFPIPNGTSSVTASWREYRDLERGSGLVAVTEYRVFGADGWSKWLNADTGM
                     SLRVGGERGWSAASAELAEAARADSVQLRFTVQCVPLLSGDFTHCSSALYGLFYDDLG
                     LMVRTGVPAPTFGLYPGGLPQSTFVDGTMTGVNCSAPPCWPGIRGSDLSGGIAIKDNV
                     NSPLGDSLVVNIRSSLRPRGMGINWKRGYDPTLNQGRTIFFTNGSYNAAFDTPRMIYR
                     LFDPATKSWSPFDSTELDANAVAVANSDTTVTDGEYRAVWPPRDKVDAAASLPGGFTI
                     NGVGAYASLAFLPRGTRLQYYFKAVDINGGIVYQFGSEDAPNEVEDLPVLPGGSARAP
                     DILEFRVLPGAYAPGAVATLLAGRTDTPVLNVDGAYTIWSYGLDPVTQALRGLGVRAD
                     RYRLLQVLGGGNGIGGHELTGLRAERRNTFFPSLLEYGIRDSLAKWYRILIVSSHTRD
                     WTVADEQDARLLSEWWDASTGTDGGDRCLFASGDDFFNSLIHPPPGYPGSYGIGMAQT
                     VFGVSAATGSWSGTGSSLYPTIDDRFAAPSAGPGLAAPGTYTYPIDGGCPGVNRFDAL
                     TKVAAPDAQSAVSYPSSEVAAIARMSERDGVTDADRNKSLGYAFSFQYIRQAGISTSA
                     SSYPHSGLENRLRVLYKFLTSCRGARTGAPSDTGKCWPCPTPGTTVPLMQANWAVQAG
                     FVTTTYGPLYPIQDNLAVTGLDELPVTESVPRVNALGQNRPNPFNPQTVIPYSLAAKG
                     RVAIRIFNIGGRLVRTLLDERQEAGPHEVRWNGATETGGHAASGVYFYRITYPDGSIS
                     AKKMMILR"
     gene            complement(6061..>7226)
                     /locus_tag="VFM00_00640"
     CDS             complement(6061..>7226)
                     /locus_tag="VFM00_00640"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7496785.1"
                     /translation="VAAGADAAYRERHAAYFLTLAEAANPELMGPSQRTWLDRLEEEH
                     DNFRHALAWTTARGEGESALRLASALDRFWWMHGHWNEGRKHLEQVLSAPAGSARSEA
                     RAEALQGAAVLARRQGAQAVAERYLNESLLIRRELGNPRGIASTLQALGSLAHDRGHG
                     EKAQALYQESLAMFRDLGDRRSEAGILHNLGNEAQAQGRYDAAASLFQEALEINREIG
                     NTVWEAMALNGLGSVARDRGELNDARAFHEQALALQRQLGDRRGIAYTSGELGSIACA
                     LGSPEEGRSLLEQNLRIVRDLGDSVWVADSLERFAVLAAAQGQAERALCLAGSAAALR
                     ESIGVPLTENDRVKLDENLAPARNGLGEEAAREALGRGRRLSLETALEYALSG"
BASE COUNT         1205 a         2401 c         2469 g         1151 t
ORIGIN      
        1 ttccctggat gaggcagcac gctgccaccc cacgcaacct tccacgatac gcccgatgct
       61 cgaaggttgc cagggacccg gaaaggcttg aattgcgggc cggatgtctc cgagaatccg
      121 ccgcatgttt cgccgtctcg ccttggccct tgcgcttctc atcgtcctcg cgggcgccgc
      181 gttctccgtc tacggggccc cccgcccgtc gaaaacgacg agggccagcc gttcgtcgtc
      241 aagcactgag catcgcatcg gagggaacga tggagcgaaa gcgagcgagt gactatccgc
      301 aagagctgct gaatctcttc gacaagtacg tccacggcgg gatcgaccgg cgcggcttcc
      361 tgatcggcgc cgcacgattc gcggtgggcg gcgtgaccgc ggcaatgctt ctcgatgagc
      421 tgtcgccgaa attcgccgaa ggacagcagg tgcctccgga cgacaagcgg ctgaccacca
      481 cgatgctcga gtacgactcg ccccagggca gcgggaccat gaagggctat ctggcgcgtc
      541 cggcggaggc gaaggacaag aagctgcccg gagtccttgt catccacgaa aaccgcggct
      601 tgaacccgca catcgaagac atcgcccgcc ggctcgcgct cgagggcttc atggcgttcg
      661 cgccggacgc cctgaccccg ctcgggggct atccggggga cgaggacaag gcgcgcgagc
      721 tgttccagaa gctggaccag acgaagacgc gcgaggactt cgtgaccgcg gcgaacctgc
      781 tgcgcgagcg ccccgactgc accggcaaga tcggcgcggt cggcttctgc tatggcggcg
      841 gcatcgtgaa cttcctcgcc acgcggatgc cggagctgaa ggccggcgtg cccttctacg
      901 gcaatcagcc ggcggctgca gacgtgccga agatccatgc gccgctgctc atccactacg
      961 cggagaccga cgagcgcatc aacgcaggct ggcccgcgta cgaggcggcg ctgaaggaaa
     1021 gcaagaagaa gtacgaagtc ttcttctacc ccggcaccca gcacggcttc aacaacgaca
     1081 ccacgccgcg ctacgacgcc gcggcggcca aactggcctg gtcgcgcacc gtcgcgttct
     1141 tcaggaagca cctgacctga gcgggcctcc gatcggcttt cggcgcggta cctcatcgct
     1201 ctggccgcac gcgtcgtgcg cgaggtcggg gatctcctcc gcggcgcgaa ggaacagggc
     1261 aaacacctcg cgacgctggc catcgactcg gaagtccgat tccgatcgcc ggccgatcgc
     1321 gccgcgttca cggcggagct cacggaagcc atcgccggac tcgtcgctcg gtaccacgac
     1381 gcggcggccc ccggcggccg cgcgcatcga ctcatcctcg tcgcccaccc cttaccccgg
     1441 aagtcccgga gctaggagcg atcgtgccta cccagaagga tccgtcaggg cggcgtttca
     1501 tcgccgtcga aaccgaagtg cccggaactc ctgaagaagt gtgggaggcc attgccacgg
     1561 gacctgggat cacgtcgtgg ttcgtcccga cccagctcga agagcgcgag aagggaccga
     1621 tcgccatgaa cttcgggccc gggatggagt cgaaggcgga gatccaggag tgggatccgc
     1681 cacgccgttt caccgccatg aaccaggaag gcatgggtcc gggggcgcca cccatggcga
     1741 ccgagtggac catcgaggcc aaggccggcg ggacctgtcg cgtgcgcgtc gtccatagct
     1801 ggttcgcgag cacggacgac tgggacggtc agttcgagag cgtcgagaag gggtggcccg
     1861 cgttcttccg catcctcaag agctatctcg cgcatttccg cggacagccg tgcacgcagg
     1921 ttcagatcat ggcggtggca cccgggccgg aggagaaggc ctggagcgag atcacgggtc
     1981 cgctcggtct cgcgggtgcg aacgcaggcc agtcggtgac gtcctcggga gatgcgcccc
     2041 gcctcgcggc cgtcgtcgag cacgcgggcc cgaagcccca cgagctcatg cttcgtctgg
     2101 agcagccggc gccgggtatc gcctatctat ccacgatgcc gatgggggat caggtgggtc
     2161 tgtggatctg cctctacctg tacggcgagg gggccgccgc ggtcgccgcg cgcgaggagc
     2221 cggtgtggca agcctggctc gctcggctct tccctgcccc ggtcgggcag gaacggtcct
     2281 agctcagcgc aggatcatca tcttcttggc cgagatgctc ccgtcgggat acgtgatgcg
     2341 gtagaagtaa accccgctcg cggcgtgccc gcctgtttcc gtcgcgccgt tccagcggac
     2401 ctcgtgcggg ccggcctcct gccgttcgtc gagcagagtc cggaccaggc gtcctccgat
     2461 attgaagatc cggatcgcga cccgcccctt ggccgcgagg ctgtacggga tcaccgtctg
     2521 aggattgaac gggttggggc gattctggcc cagcgcgttg acccgcggga cggattcggt
     2581 gaccggaagc tcatcgaggc cggtcacggc cagattgtcc tggatcggat agagcgggcc
     2641 gtacgtggtc gtgacgaacc ctgcctggac cgcccagttc gcctgcatga gcggcacggt
     2701 cgtgcccgga gtcggacagg gccagcactt cccggtgtcg gagggggcgc cggtgcgcgc
     2761 tccgcggcag ctcgtcagga acttgtagag gacgcgcagc cggttctcca gtccggagtg
     2821 cgggtagctc gacgcggagg tcgagatgcc ggcctgccgg atgtactgga acgagaatgc
     2881 atagcccagg ctcttgttcc ggtccgcgtc ggtaaccccg tcgcgctcgc tcatccgcgc
     2941 gatggcggcg acctccgagc ttggatagga gacggcgctc tgcgcgtcgg gcgcggcgac
     3001 tttggtgagc gcgtcgaagc ggttcacgcc tgggcaccct ccgtcgatcg gataggtgta
     3061 cgtccccggc gcggcaaggc cgggaccggc gctgggcgcc gcgaaccgat cgtcgatcgt
     3121 cggatagagc gagcttcccg ttccgctcca ggagccggtc gccgcggaga cgccaaagac
     3181 cgtctgggcc atgccgattc cgtaggatcc cgggtacccc ggaggcggat ggatcaggct
     3241 gttgaagaag tcgtcgccgg aggcgaacag gcagcgatcg ccgccgtcgg tgccggtgct
     3301 cgcgtcccac cactcgctca agagacgcgc gtcctgttcg tccgccacgg tccagtccct
     3361 cgtgtgggac gagacgatca ggatccgata ccacttcgcc agagaatccc tgatgccgta
     3421 ttcgaggagg ctcgggaaga aggtgttgcg ccgctccgcc cgtagcccgg tcagctcgtg
     3481 ccctccgatt ccgtttccgc ctcctaggac ttgaaggagc cggtagcgat ccgcgcgcac
     3541 gcccaagccg cgaagcgcct gcgtcacggg atcgaggcca taggaccaga tcgtgtacgc
     3601 gccgtccacg ttgaggacgg gcgtgtccgt ccttccggcc agcagcgtgg ccacggcgcc
     3661 gggcgcgtag gcgccgggca ggacccggaa ctcgagaata tcgggagccc tggcggagcc
     3721 gcccggcagg accggcagat cctcgacctc gttcggcgcg tcctcggatc cgaactgata
     3781 gacgatcccc ccgttgatat ccacggcctt gaagtagtac tgaagccgcg ttccccgcgg
     3841 gagaaacgcg agcgaggcgt aggctccgac cccgttgatc gtgaatcctc ccggcaagct
     3901 cgcggccgcg tccactttgt cccgaggcgg ccagacggcg cggtattcac catcggttac
     3961 cgtcgtgtcg ctgttcgcca ccgcgacggc gttggcatcg agctcggtcg aatcgaaggg
     4021 gctccagctc ttggtggccg ggtcgaagag gcgatagatc atgcgcggag tatcgaacgc
     4081 ggcgttgtag gacccgttcg tgaagaagat cgtccgtcct tggttgaggg tcggatcgta
     4141 cccgcgtttc cagttgatgc ccatgccgcg gggccggagc gaagagcgga tgttcacgac
     4201 gagggaatcc ccgagagggc tgttcacatt gtccttgatg gcgattccgc cgctcagatc
     4261 gctcccgcgg atgccgggcc aacagggagg agcggagcag ttcacgcccg tcatcgttcc
     4321 gtcgacgaag gtgctctgcg gaagcccgcc cgggtacagg ccgaacgtgg gcgccggcac
     4381 gccggtgcgc accatgaggc ccaggtcgtc gtagaagagg ccgtacagcg ccgaggaaca
     4441 gtgcgtgaag tcgcccgaaa ggagcggcac gcactgcacg gtgaatcgga gctggacgga
     4501 gtccgcccgg gcggcctccg cgagctccgc cgaggccgcg ctccatcctc gttcgcctcc
     4561 gacccgcagc gacatgcccg tatcggcgtt caaccacttg gaccatccat ccgcgccgaa
     4621 gacacggtac tccgtgaccg cgacgagccc cgatccgcgc tcgagatccc ggtattcccg
     4681 ccagctcgcg gtgacggaag aggtcccgtt cggaatcggg aacgtgcacg tgacgatcga
     4741 ggcgaacgag cccggatcgg tcatgccggt gccgaagtcc cccgcctcga agaagagatc
     4801 cgagccgagc cccttggccg gggagcagaa atcggtggtc ggcttgctcg aaccgaccgc
     4861 gagatgccag agcgtcccga tggcggtggg aacgcgggcc gaaacggtcg gcgagcccgg
     4921 tgtcccgacg atcacatcgc ctccgaatcc gtccgttccg ccgacggccg cctggttcga
     4981 gtagatcgag ccgttgtcgc tcgtggccag gagatcaagc tcggcgccgt tcccgttggg
     5041 ccagagcccg tcctccgagg acatccgctc ctcgctggtg aagacgaagt agagcgcgcg
     5101 gtgcccgggg gcgatcgtga tcgacgtgga gaccgttgcc ggcgcgccac cctcggcgcc
     5161 gaggattgag accggacccc cgagcgtgga gatggaggcc gcccccggcg tgctcggggt
     5221 gatcgtcccg gtccaggtga ccaggagctc ggaggcgcca ttcgatccgt tctcgatgac
     5281 gttatccaga acggggcggc tgtttccgat cggatcaacc gcggctccgc ctccgccgat
     5341 caggtacagg aagtcatatc cgggctcggt gctcccctgg aagagaagcg tcagcgtata
     5401 ggaccccgag tgagcgccgg tgtcgacgta caggatctgg taggtgacgt tcgggtatcc
     5461 cacggtctga accgccttgg gatcgtactt tccgcaccag aggcttcgcg tcccttcgag
     5521 aggagccggc cgctgcgacg cggggacgcc tgtatcgaag acgtgccaga gggtctcctt
     5581 cagcgattga tcctgcgccg tgtagggacc tgtggtgccg gcggaatagg agttcaagga
     5641 gtccgcgacc ggactcgtgc gcggggacca ggtattgagc gcccgctccg cgcaggcgtt
     5701 cgggtagagg aaccacgagg tcgtggtggc ggattgtttg agaagcgtcc ccgagaggag
     5761 cgccttggga ggagcgagga gggtgctgcc ggaggtcgac ccgtcgtaga ggatcgcggc
     5821 cttcgtctct tgctccttgg ccgctcccgg tgagggtagg cccagaagca ggagaccgac
     5881 gacgaggacc gcgcgaagcg gcgaagcggt cgagtggcgc aaggccaggg ctggattgac
     5941 gacgggaaga acgaccccac gacggggtcc acaaggaata cggcatccgt ccatgcgcac
     6001 gttctctccg ccgtgcgccc ccaaacggat gaggcatcct agcaccggag gaggtgcgag
     6061 tcaaccgcta agtgcgtatt ccaacgcagt ttccagcgag agacggcggc cacgcccgag
     6121 cgcctcccgg gctgcttctt cccccaggcc atttcgggcg ggggccaggt tttcgtcgag
     6181 cttcacgcga tcgttctccg tcaggggaac gccgatcgac tcccggagcg cggccgcgga
     6241 gcccgcgagg cagagcgcgc gctccgcctg tccttgggcc gctgccagga cggcgaaccg
     6301 ctcgagagaa tccgccaccc agacactgtc ccccagatcg cgcacgatcc ggagattctg
     6361 ctccaggagc gatcgcccct cttcgggcga tccgagcgcg caggcgatgc ttccgagctc
     6421 gcccgacgtg tacgcgatcc cccggcggtc tccgagctgc cgctggagcg ccaacgcctg
     6481 ttcgtggaac gcgcgagcgt cattcaactc tccccggtcg cgcgccacgc tgcccaggcc
     6541 gttcagcgcc atcgcttccc agaccgtatt gccgatctcg cggttgatct cgagggcctc
     6601 ctggaacaag gaagcggcgg catcgtagcg gccctgcgcc tgagcctcat tcccaaggtt
     6661 gtggaggatt ccggcttcgg accggcggtc cccgaggtcg cggaacatgg ccagactctc
     6721 ttgatagaga gcctgcgctt tctcgccgtg gccccggtcg tgagcgagac ttcccagcgc
     6781 ctgcagggtg gatgcgattc cgcggggatt gccgagctcg cgccggatca ggaggctctc
     6841 gttcaagtag cgctcggcta ccgcttgggc gccctgacga cgtgcgagaa cggcggctcc
     6901 ctgaagcgcc tcggcgcgcg cctccgaccg cgccgagccc gccggagccg acaggacctg
     6961 ctccagatgt tttcggccct cgttccaatg gccgtgcatc caccagaacc ggtcgagggc
     7021 ggaggcgagc cggagcgcgc tctcgccctc ccccctcgcc gtagtccagg cgagcgcgtg
     7081 gcggaagttg tcgtgctcct cctccagccg gtcgagccac gtccgctgcg aagggcccat
     7141 gagctccggg ttcgcggcct cggcgagggt aaggaaatag gcggcgtgcc gttcccgata
     7201 cgcggcgtcc gctcccgcgg cgacca
//
LOCUS       DASSLZ010000020        22102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_768752, whole genome shotgun sequence.
ACCESSION   DASSLZ010000020 DASSLZ010000000
VERSION     DASSLZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 22102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22102
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768752"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2983
                     /locus_tag="VFM00_00645"
     CDS             <1..2983
                     /locus_tag="VFM00_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496786.1"
                     /translation="ALALLTWALLPSAAEAGPYSRLQVLLPGETAAPGTASGKSGTPR
                     AQITGTPFTVTVNACDASWNVITTNSNSISIDATDASATLPAPAQLVSGTRTFSVTIN
                     AAGTFTIGAHDQTDNTIPPASSSAVTVRILDHFIFSGISQKNQYAGTSMPLTVTAKDP
                     SGNTVAGYSGPVVLQEITSFGPGRITASPGPDSVITMTNGVWSGNVTMYRADETSINR
                     GNVNVYAYDQAHPTKNGTSDPFTVHPGTFSRVQIVVPGQSPYPGSLSGVAGAPATQGA
                     GTNFNVSCYSTDPYWNQVPSGDQVRVTSSDGATNTSGTLSNGFVQLTVHLNTVGTQTL
                     TITDLTTSKTPMTSAGIQVVPAGVTKFVFNTITGPLTAGTPVSVTVRATDASSNTVPG
                     YNGDAILQANTGNQSITPTNITFANGTWTGNVTFKGAGGAVALTCADFSAPPKTGTSN
                     TFVVNPGPFAGLQVILPGETALGGTPTGKTGTPTTQSAGTPFTVTVRAVDQYWNLVPG
                     VNDRIVLTSTDAFAWMPSDTVLVNGQVLIPTRLHKSGLQTITARDSTNSGINPNTSSQ
                     VNVIGGTFARLLILAPGETPAPGTATGRAGTATDQSINYAFNVTVLATDQWWNPVGGA
                     SDVVHITSGDPLATLPPDQAMVNGTVDLPVRLATGGFQQISVTDATNTGKTGSTTQVR
                     AISTGFHLQADITPSTAKAGEPFTLTVKVTNDAGSVIQEINSFVTVIVKNAGSQAAGR
                     GTLLNTQFQLLQGQRSISETYTFVEPIIIEAHDDAGNAPATSNVITITPGDPAAIRMS
                     SNPAWVGGNKHATVYARVVDAFENGVPDQPVVFSLLSGTGTLTPMDTTSASDGNARAD
                     FLSPRQPEMDQIRATSGSLLNDLNLETAFVDPTKGGGYATNYPNPFHPPSEGTTIAYK
                     LDDNASVTMRIYTQTGSLVKRMFFDKGVTGGLAGLNEVVWDGKNGTGDVVASGGYVVL
                     IEAQGTGETLHVIRRKIAVVR"
     gene            3001..4257
                     /locus_tag="VFM00_00650"
     CDS             3001..4257
                     /locus_tag="VFM00_00650"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET7496787.1"
                     /translation="MVVKSRHHMLSVALAAALVLTAGAARAEDETGGAPGSWLSQYVS
                     ARTMGLGGAFVGAADDASAVAWNPAGLALLVPNEARFESARLFEDTSVNALNFAVPGN
                     KLPCFAVSVISLHSGAFDRTNELNDPLGTFSEGETAYIFSLARAINPRFALGTNVKLI
                     RQTVEDFSGTGLGFDVGAVANVTPNLRVGASVLNVAGPGITLRDTKETYPIEFRGGFS
                     ATVLRGRGLLTAEVDQSQGPGVRFRGGSEYWIQPMFALRVGMDDQNPAGGMSYRFGGH
                     YQFDYGVLDHPLGMEHRIGLTYRFGGFFASAKADPEIFSPTGERATTKIDLNARTKGE
                     TDTWALELINKSDETVRKFGGKGVPPAHIEWDGKDETGLPLPDGTYRYRLTVTDTQGR
                     VIESHVRTVTISTGGPQGEVPVIPVQ"
     gene            4277..7420
                     /locus_tag="VFM00_00655"
     CDS             4277..7420
                     /locus_tag="VFM00_00655"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7496788.1"
                     /translation="MTHMDEQTTTRSGARAARLAALLLALAASSVSGPARAEAPKPPA
                     GLRGAALSFETARLLPEATRAAGLDEADRLAVEAAKGARNEDEDVAARYLWAQILLEK
                     GDKARASEALHQSADRLGKSPYADDAELQSIQALEAQGKDADAAKAWVEWEKRFPQSP
                     LWGEAKLAQAWNALRRGQNAPADKLLTALLAKQPWYGNDARVQLARATALYQNGKAAE
                     ALALVGPKPGGSGGAAAAYLRALCHRSLGSMLASAAAFQEVAARYPDSPLRDPALLGK
                     ADAFLRARDYRSAAEEFARAAKQAKDPNVAAEAEVRAAGAVSLTGAADSALALFHGIV
                     ERRPGTDQAARAQFLIGDMLATRGQYAEAIVELNRVLTNYFQHSVAASAQYRVARCLD
                     ALGRKADATGSYQAVVSGYPLEPEAPAAAYMAGVGLLAQNRPRAAAPYFQIVLDRYVA
                     SAMDSSRSVRPITPERQELIDAALCLLLYSYRTAGDLGQVAGAPHLLLQKMPPSHNPW
                     RAYALLIDADAMAAQSRHPEAQATLEKLIHDFPDHPVGASAAKLLAWTYAQQGKDSLA
                     VQTEEKLVLRYGATDQVVVAGALLDIAHDRFNQKRYREAAAGYEDFLKRFPASPKRHL
                     ANYQAGLAYVRLNRLGDAVDHWEAIVRDSSKAPIAEKAWARAGDLYFQAQRYNDAKRC
                     YRGLLENFATTDAAALATLRMGQCEYNAGNDAAALESFSQAMARFPDSPYAKEAKRGT
                     ELALYRLGQSDKGTAVLAQLVEQYPNSAYAADALFQIAKNDYQAKRYDKASDGFRRVV
                     SQFPGYSAADQAQYLLADALDKAGKPAEARQASEQFLAFFPESDLRPTVQFQVGLQQF
                     QNKEYAPAAVAFTQVLADSAAPDVRAAARYNLALCQRSLGQNDEARATLEAYRNERPA
                     DSRAEEVAYQLGDIHDGAGRYAEAQSEFEKALDGKPSAALETELRFRVGRCLEEQQKI
                     DEATRWYIRASQAVDRRNAYRLSAVARLAAIYESKREFGKAMAAYQDIASYSKDRELA
                     QAAAGRATQLRSSRTTR"
     gene            7450..8091
                     /locus_tag="VFM00_00660"
     CDS             7450..8091
                     /locus_tag="VFM00_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7496789.1"
                     /translation="MFENFDWIQAMRTSPVMVVILFCSVLTAGFALERALYYWKRRGN
                     PDALLASALVAMRSGNREEAVRVLSSNPHPMGAVAAELVKNLDQPEGVIEEKMHIALS
                     EQRMQLDRNLGFIGTMGNTAPLIGLLGTVWGIMRAFHDMARTGSAGPSVVAAGVAEAL
                     FTTAAGLCVAVPAVIIYNHFVRRMGVMLTVAENNARTIRMNATASSHQAHRAA"
     gene            8102..8515
                     /locus_tag="VFM00_00665"
     CDS             8102..8515
                     /locus_tag="VFM00_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7496790.1"
                     /translation="MAGRRWRMRNPESIFEPNMTPLIDVCLVLVVILMVATPMAFQSS
                     IGVQKAAKAGKAAAIAAKSERVEISIVSADTVTVNRTTVVRKDLPLLLKPLLQLSNTK
                     TVIVHCADTVPHGAFVGVLDEAKQCGAAQIAVVGG"
     gene            8517..8939
                     /locus_tag="VFM00_00670"
     CDS             8517..8939
                     /locus_tag="VFM00_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7496791.1"
                     /translation="MRSFTPRQTLITKVNVTPIIDVALVLVIILLVTAPMLAVSDLPV
                     NLPQAQTRESEDERNLSITLSSSGELALDEARVTKETLTAAIHERLQKKGDENVLVVV
                     RADEDAPYSVIRELLDDARAAGATRIAIATRQKTGTPR"
     gene            8936..9871
                     /locus_tag="VFM00_00675"
     CDS             8936..9871
                     /locus_tag="VFM00_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET7496792.1"
                     /translation="MTAATLRLDLMECHTRTRRTMIASVVLHALLFLWIATFKTAEVL
                     TPPITEITMLSAGDLATIDAGPAEPAPRVVPRPGLAPRKVDVDQRFVRSADRADITLD
                     PQSPTSIADQLSARLSRLTQEERPAVPGTVVAPPSAVFGSGPVTAKGMGTGTPVALSR
                     GGALGGAGALPLNRGGGGGGSAASGPATLPAVKTAATAPAQVGDATARRTIAGASVAG
                     PIADRAVLQTVVPEYPEWAKKEAVEGSVTLYFVVRSDGTVKENVVIQKTAGFGDFDEN
                     ARTALRGWLFEPLRGGRTGEQWGTITFHFRLRDAG"
     gene            9885..11276
                     /locus_tag="VFM00_00680"
     CDS             9885..11276
                     /locus_tag="VFM00_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496793.1"
                     /translation="MNLRTYRDTVAAAAAAALTLLFVAHHATAGTTGTAKPSVTVGTV
                     SVVTPTPAGPETSAAVPGTPAAKAPAAKPAAVTPKRKAAKKSAAKPAPAPSEAANAAD
                     AKGSVGATAGAADAKGGAPNAAGATPAGNATPAGNATPNAAAADAKDGEKGYTIKGGA
                     ERTDLRSLTIEGEDRVHVEIERPPLALQLDPAKVNGLDFGTAQDVLDRTPPDLTTQYL
                     AVSANERSPYTGRPWLRQFASGSVARFQPNVRGTERWKLTVADSHGQTVAQFGGKGEA
                     PKEIVWDGRSLSGAPVTPGITYSYVFEAVDKAGNKRNFVGQGFKVSAYRLDSPLGPTL
                     VFSAQTLQEGAVATAYGPTGAATRPAPPIVLEAASTLNQSPRVSQPIRVNVTGRTYEY
                     ANATAKQVAAWIADLTIGDPARVQAVAQVEPDAPEGGTVRIALDGVGVAGNAVPTMKA
                     PKVEKSGKKSKKG"
     gene            13362..14051
                     /locus_tag="VFM00_00685"
     CDS             13362..14051
                     /locus_tag="VFM00_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496794.1"
                     /translation="MAVQNGVAWFYGHRFEAPFTLEYCDDGLAINGSFLPREVRLFPK
                     SRPGHGDTLRAELSARALEILRDAQRNHVPRDEAFAQIEQLYRSCPLVKRAELRGYSL
                     RTSYKDRSGDYILQLVDPERVAMRDMALEESSLKERGTQSKHLLQLKDHLESAGSILW
                     FDSPGYVLLPKDDSARIDAILQRMQQKEKITKEEGAFLQSLIPASLGELSRMAEQPKR
                     LLTLFPHPEAR"
     gene            14048..14728
                     /locus_tag="VFM00_00690"
     CDS             14048..14728
                     /locus_tag="VFM00_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7496795.1"
                     /translation="MTDAPKDPERRATFILARGEKWLLPRLAASLPRWMVPDHLTAIG
                     ILGATLIALGYILSTRDRHWLWLSNAGLIVNWFGDSLDGTLARVRKHERPRYGFYLDH
                     LTDAYSTTAVGLGLGFSPYMLLSVGLAIVIAYLILSINVYLETHVFGVFRYGYGVLGP
                     TEARGVLFLLNIVALVTGPIPFTIHGIRFTAFDVAGASAALSMGGLLLRRVVRNLGEL
                     ARMEPPRS"
     gene            14823..16415
                     /locus_tag="VFM00_00695"
     CDS             14823..16415
                     /locus_tag="VFM00_00695"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET7496796.1"
                     /translation="MARTGEPTGTRVLRALRRAATRQTVRREFERQTGPSDLERSVRE
                     YCGDTRIVIASNREPYQHVHGAHGIEVIRSPGGLASALDSVARATNATWVAQATGDAD
                     RETADAQGRVAMPPGSNRYTLQRVWIEPEAHADGYNRFANGCLWPLCHVVYVRPHFLW
                     DEWRDYREVNEQFADAIAASVGPEPALVLLQDYHLALCASRLRQKRPDLSIVLFWHIP
                     WPNPEVFRILPWKLEVLEGLLACDLLGFHIPHHAMNFVDTVATELEAHIDRERSAVRR
                     RGHRTFVRSYAIGPDVAEISMAAADPTTRREAERVRETLGLGGARVILGVDRLDYTKG
                     VPERLDAFGRFLELYPDRREDVVMVQIGVPTRVNLPEYQALMADVEQRVERLNAAYGR
                     NGRNVVHLITRNLDFRELIPYYVLADVMAVTSLHDGMNLVAKEYVAARPGGDGVLILS
                     PFTGASRELEHAIQVSPYDREGLAVAFQRALTLSPAERADRMRSLREVVAAQNVYDWA
                     RKLIRDVRRLHLLPGMRPAGRR"
     gene            16412..17215
                     /gene="otsB"
                     /locus_tag="VFM00_00700"
     CDS             16412..17215
                     /gene="otsB"
                     /locus_tag="VFM00_00700"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET7496797.1"
                     /translation="MTAARRRAPRRLLADGAWEDAEASLARAARAGRPALVALDFDGT
                     LAPIVSRPDRVRVPSATLRSLRDASKLPWLRIAALSARPMRDLARYLPVTGILRVAQY
                     GLEGGDALDPKRLRAIRAGVDALHGWIEPLAAAAPGAWIEDKRLTIAVHYRDVAPRHR
                     GALVRRLRTLAARAERLGFHVERGRRVLDFVPAGYDKGRALRMLRRRVRPAVTFYFGD
                     TAGDEPAFAALGRRDFPVRVGTGPTAAPYRVRGPEEVARFLRALVSKRR"
     gene            17220..17915
                     /locus_tag="VFM00_00705"
     CDS             17220..17915
                     /locus_tag="VFM00_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496798.1"
                     /translation="MSWDSVIPPDLALQLHDTVVSVVGGIILVLVAGAIAFLGWLVAT
                     LVGRAAQWILSHTGVDASTQKLSPGEGRRVELYPSRLVGYALFWTIFLSACIVALRVV
                     GLDLAPSIAARLQDVVPRVLTSAVVLILGIPLALAGSRILNALLAPSGLKPNRIRSQA
                     VSALLIGFVVLVALEQLGLAAQLVIAIGITVVAAAGLALALAFGLGCRDLARDLIVEY
                     LRAGEEGPHPGRP"
     gene            17962..18741
                     /locus_tag="VFM00_00710"
     CDS             17962..18741
                     /locus_tag="VFM00_00710"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HET7496799.1"
                     /translation="MSDDRFAFCSVAHLEAPSGIVALSMDALRAGIERAPDDSLFQHV
                     TCVAMRYPHARDLPSNDFARWVGTSLQAPDVAERLAYAGAATAQPIRELRASLLSVLD
                     SVPQPERSREAPPEAAFHFVGVRSVPVPLGIEASDPVQAIDVWHRLDSAALFFHVIEA
                     PVFGDAANSFPHWLRRHGADALAIQAEQAVAAGRPIARLRKDLGARWRRSQIGRRLAE
                     RAGAPEEERLREARAAMARLAGRLRGGAGKRRNDGGPEERP"
     gene            18738..19988
                     /locus_tag="VFM00_00715"
     CDS             18738..19988
                     /locus_tag="VFM00_00715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7496800.1"
                     /translation="MSAIRDAARVPYEHPPAEHWLARYEPVVGAGTLRALEALARKLR
                     GHRIVMVNTTKTGGGVAEILHRVVVILNELGVPTTWEVMEGDDRFFGVTKRMHNALHG
                     HVEPLTEQDREIYHERTRLEADRLALDGDLIFIHDPQPAALIEHRRQPGQKWVWRCHI
                     DLSRRDPAYWDFLQPYVSRYDASIFSHIAFVPPITIPAYLVPPSIDPFATKNRDLSEH
                     EIEGVLERLDLSPGPGWITQVSRFDRIKDPIGVIDAYRLVRARRPNARLLLAGGGADD
                     DPEGAEVLAEVRARATAAPGVTVLELPPGSDLEINAIQRASTVVLQKSLREGFALTVS
                     EALWKRRAVVASAVGGIPLQVLHEQTGLLVHSIEGTAFQTIRLLDNAELRRSLGDEGR
                     QHVRDNFLHTREVRDYLAVFASLV"
     gene            complement(19958..21664)
                     /locus_tag="VFM00_00720"
     CDS             complement(19958..21664)
                     /locus_tag="VFM00_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7496801.1"
                     /translation="MRANVLGHRIRSLMPVADDRSTRLLGAPTETAVPPAPEPGPVLF
                     AWGPLQVLERVGEGAGGEVYRAFDPGLRTEVALKLLKPDASWSPAARERFQGEARKLA
                     QVRHPNVLAVHGVGEHDGRMGIWTDFIRGCSLEEYIRREGALEAGEAARIGLDLCRAL
                     AAVHASGLVHCDVKPSNVMREQGGRILLMDFGCVAELRSAAGMEQRVEEASGTPVAMA
                     PERLRGAPATARSDVYGLGVLLYRLVTCRYPVEGRSVSELLRLHERGAGVPLSDRRPD
                     LPPDFVQVVDRALSPEPSRRYLSAGAMGRALTTALEARGTGSRRLVARSVGVAVIAVA
                     GLVAVSWLIGSRSAHRDVRPAGARPAGTSASGNASWAAKPLQASASLFRHLGARDEPL
                     PSGATIAPGDRLFLEVSSEQPMHTYVLDEDEAGDVYGLFPIPGLEASNPLSSRVRHLL
                     PGPAGDSLAYWTVTSFGGRERIVVIGSRSPRHEVEEMIARVPSAIPGRPIRLVSIPVA
                     ENVRGIGGVEKQRAFATTHRKLDEMLRSLESRRQDSGDVWIWRVELRNPAPHTRDAKT
                     AR"
     gene            complement(21718..>22102)
                     /locus_tag="VFM00_00725"
     CDS             complement(21718..>22102)
                     /locus_tag="VFM00_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017242445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496802.1"
                     /translation="ILARLREEAARLGLKLAQANAGDPVCYAAHANSFLVRADGRLNK
                     CTVALELPANQVGRIREDGTLDLDRTKTLAWSRGLDSGSAKELACPLQGIGAVQASAW
                     SARAAGQTGAAAGMRHGVAVVEAQV"
BASE COUNT         3484 a         7876 c         7556 g         3186 t
ORIGIN      
        1 cgcgctcgcg ctcctgacgt gggccctgct gccatcggcc gcggaggcgg gtccgtactc
       61 gcggcttcaa gtgttgctgc ccggcgagac cgccgcgccc ggtacggcgt ccggtaagtc
      121 gggtactccg cgcgcccaga tcacgggcac tccgttcacg gtgaccgtga acgcgtgcga
      181 cgcgagctgg aacgtgatca cgaccaattc gaattccatc tcgatcgacg cgaccgacgc
      241 gagcgccacg ctgcccgcgc cagcgcagct cgtctcgggc acgcggacgt tctcggtcac
      301 gatcaacgcc gcgggtacct tcaccatcgg cgcgcacgat cagacggaca acacgatccc
      361 gccggccagc tcgtcggcgg tcacggtccg cattctggat cacttcatct tctccggcat
      421 ctcgcagaag aaccagtacg cgggcacctc gatgccgctg acggtcacgg cgaaggatcc
      481 ctcgggcaac accgtggccg gctactccgg tcccgtcgtg ctgcaggaga tcacctcgtt
      541 cgggcccggc cggatcacgg ccagcccggg ccccgattcg gtcatcacga tgacgaacgg
      601 cgtctggagc ggcaacgtca cgatgtaccg cgcggacgag accagcatca atcgcggcaa
      661 cgtgaacgtc tacgcctacg accaggccca ccccacgaag aacggaacga gtgatccgtt
      721 caccgtgcac cccgggacgt tcagccgggt gcagatcgtg gtgccgggac agagccccta
      781 cccgggcagc ctctcgggcg tcgcgggcgc cccggctacg cagggcgcgg gcaccaactt
      841 caacgtctcc tgctactcca cggacccgta ctggaaccag gtgccgagcg gcgaccaggt
      901 gcgcgtcacg tcgagcgacg gcgccacgaa cacgagcggc acgctgtcga acggcttcgt
      961 gcagctcacg gtccacctga acacggtggg cacgcagacg ctgacgatca cggacctcac
     1021 cacatcgaag acgccgatga cgagcgccgg aatccaggtg gtcccggcgg gcgtgaccaa
     1081 gttcgtcttc aacacgatca ccggcccgct cacggcgggc acgccggttt cggtcacggt
     1141 gcgcgccacg gacgccagtt ccaacacggt ccccggctac aacggcgacg cgatcctcca
     1201 ggccaacacc ggcaaccaga gcatcacgcc gaccaacatc accttcgcga acggcacctg
     1261 gacgggcaac gtgacgttca agggcgccgg cggcgcggtc gcgctcacct gcgcggactt
     1321 ctcggcaccg cccaagaccg ggacgagcaa caccttcgtc gtaaatccgg gcccgttcgc
     1381 gggccttcag gtgatcctcc cgggcgagac ggctctgggc ggaacgccga ccggtaagac
     1441 tggcacgccg acgacacagt cggccgggac gccgttcacc gtgacggtcc gcgccgtgga
     1501 ccagtactgg aatctggtgc cgggcgtgaa cgaccggatc gtgctcacgt ccaccgacgc
     1561 gttcgcgtgg atgccctccg acacggtgct ggtgaacggg caggtgctga tccccacgcg
     1621 gctccacaag agcggcctcc agacgatcac ggcccgcgac tcgacgaact cggggatcaa
     1681 cccgaacacg tcgtcgcagg tcaatgtgat cgggggcacg ttcgcgcggc tcctgatcct
     1741 ggcgccgggc gagacgccgg ctccgggcac cgccaccggc cgcgcgggaa cggcgaccga
     1801 ccagtcgatc aactacgcct tcaacgtgac cgtgctcgcc accgaccagt ggtggaaccc
     1861 ggtcggcggc gcctcggacg tggtgcatat cacgtccggc gacccgctcg cgacgctgcc
     1921 tccggaccag gccatggtga acggcacggt cgacctgccg gtccgcctgg cgaccggcgg
     1981 cttccagcag atctcggtca ccgacgcgac caacaccggc aagaccggca gcacgacgca
     2041 ggtgcgcgcc atctcgaccg ggttccatct ccaggcggac atcacgccct cgaccgccaa
     2101 ggcgggcgag cccttcacgc tgaccgtcaa ggtcacgaac gacgcgggga gtgtgatcca
     2161 ggagatcaac tcgttcgtca cggtcatcgt caagaacgcg ggcagccagg ccgcgggccg
     2221 gggcacgctg ctgaacacgc agttccagct gctccagggc cagcgctcca tctccgagac
     2281 ctacacgttc gtcgagccga tcatcatcga ggcgcacgac gacgccggca acgcgccggc
     2341 cacgagcaac gtgatcacca tcacgccggg cgatccggcg gccatccgga tgagcagcaa
     2401 tccggcctgg gtgggcggca acaaacacgc gaccgtgtac gcgcgcgtcg tggacgcgtt
     2461 cgagaacggc gtccccgacc agccggtcgt cttctcgctc ctctccggca cgggcaccct
     2521 gaccccgatg gacacgacca gcgcgagcga cggcaacgcg cgcgccgact tcctgagccc
     2581 gcgccagccc gagatggacc agatccgggc gacgtcgggt tcgctgctga acgatctgaa
     2641 tctcgagacc gcgttcgtcg acccgaccaa gggcggcggc tacgccacga actatccgaa
     2701 cccgttccat ccgccctccg agggcacgac gatcgcctac aagctggacg acaacgcctc
     2761 ggtgacgatg cggatctaca cgcagaccgg ctcgctggtg aagcggatgt tcttcgacaa
     2821 gggcgtcacg ggcggtctcg cgggtctgaa cgaggtcgtc tgggatggca agaacggaac
     2881 gggcgacgtg gtcgcgtcgg gcggctacgt cgtgttgatc gaggcccagg gaacgggcga
     2941 aacgctgcac gtcatccgcc gcaagatcgc ggtggtccgg tgagcggggg aggccgagcc
     3001 atggtcgtca agtcgcgtca tcacatgctg agcgtcgcgc tcgcggcggc tctcgtcctg
     3061 acggcgggcg ccgcacgggc cgaggacgag acgggcggcg ctcccgggtc ctggttgtcg
     3121 cagtacgtgt cggcgcgcac gatgggtctg ggcggcgcgt tcgtcggcgc cgcggacgac
     3181 gcgtcggcgg tcgcctggaa tcccgccggg ctggcgctcc tcgtgccgaa cgaggcgcgc
     3241 ttcgagtccg cgcggctctt cgaggacacg tcggtcaacg cgctcaactt cgcggtgccg
     3301 gggaacaagc tcccctgctt cgccgtgagc gtgatctcgc tccactccgg cgcgttcgac
     3361 cggaccaacg agctgaacga cccgctcggc accttcagcg agggggagac ggcctacatc
     3421 ttctccctgg cgcgcgcgat caatccccgc ttcgcgctgg gcaccaatgt gaagctgatc
     3481 cgccagacgg tcgaggactt cagcggcacc ggcctcggat tcgacgtggg tgccgtggcc
     3541 aacgtgacgc ccaacctccg ggtcggcgcc tcggtcctga acgtggcggg cccgggcatc
     3601 acgctccgcg acacgaagga aacctatccg atcgagttcc gcggcggctt ctcggccacg
     3661 gtgctgcgcg gccgcggcct cctcacggcc gaggtagacc agtcgcaggg accgggcgtg
     3721 cgcttccgcg gcggatcgga atactggatc cagccgatgt tcgcgcttcg cgtcggcatg
     3781 gacgaccaga atcccgcggg cggcatgagc taccgcttcg gcggccacta ccagttcgac
     3841 tacggcgtgc tcgaccatcc gctcggcatg gagcaccgga tcggcctcac ctaccggttc
     3901 ggcggcttct tcgccagcgc gaaggccgac cccgagatct tctcgccgac cggcgagcgc
     3961 gcgacgacca agatcgacct gaacgcgcgc accaagggcg agaccgacac gtgggcgctc
     4021 gagctgatca acaagtccga cgagacggtc cgcaagttcg gcggcaaggg cgttccgccc
     4081 gcgcacatcg agtgggacgg caaggacgaa acgggcctcc cgcttccgga cggcacgtac
     4141 cgctaccgcc tgaccgtgac cgacacgcag ggacgcgtga tcgagagcca cgtgcggacg
     4201 gtgaccatct cgaccggcgg cccgcagggc gaagtcccgg tcattccggt ccagtaacca
     4261 cacgggggcg agggcgatga cgcacatgga cgagcagacg acgacccgga gcggggcgcg
     4321 cgcggcgcgc ctcgccgccc tccttctcgc gctggccgcg tcttcggtct ccggtccggc
     4381 gcgggccgag gcgcccaagc cgccggcggg tctccgcggc gcggcgctct ccttcgagac
     4441 ggcgcggctc cttcccgagg ccacgcgcgc cgcggggctg gacgaggccg accgcctcgc
     4501 cgtggaagcc gcgaagggcg cgcggaacga agacgaagac gtggccgcgc gctacctctg
     4561 ggcacagatc ctgttggaaa agggcgacaa ggcgcgcgct tccgaagcgc tccaccagtc
     4621 ggcggaccgg ctcgggaaga gcccctacgc cgacgacgcg gagctccagt cgatccaggc
     4681 gctcgaagcc cagggcaagg atgccgacgc ggcgaaagcg tgggtcgagt gggagaagcg
     4741 cttcccgcag agcccgctct ggggcgaggc gaagctcgcg caggcgtgga acgccctccg
     4801 ccgcggccag aacgcgccgg cggacaagct gctgaccgcg ctcctggcga agcagccctg
     4861 gtatggcaat gacgcccgcg tgcagctcgc gcgcgccacg gcgctctacc aaaacggcaa
     4921 ggcggccgag gcgctcgcgc tggtcggccc caagccgggc ggctccggcg gcgcggcggc
     4981 ggcgtatctc cgggcgctct gccatcggag cctcggctcg atgctcgcgt cggccgcggc
     5041 gttccaggag gtggcggcgc gctatcccga ttcgccgctg cgcgacccgg ccctgctggg
     5101 caaggcggac gcgttcctgc gcgcccgcga ctaccggagc gcggccgaag agttcgcccg
     5161 cgcggcgaag caggcgaagg atccgaacgt ggccgccgag gccgaggtgc gcgccgccgg
     5221 cgccgtctcg ctgaccggcg ccgccgattc ggcgctcgcc ctcttccacg gcatcgtcga
     5281 gcgccggccg ggcaccgacc aggcggcgcg cgcgcagttc ctgatcggcg acatgctcgc
     5341 cacgcgcggt cagtacgccg aggcgatcgt cgagctgaac cgggtcctca cgaactactt
     5401 ccagcactcg gtggcggcca gcgcgcagta tcgcgtggcc cgctgcctcg acgccctggg
     5461 acgcaaggcc gacgcgaccg gctcctacca ggcggtggtg tcgggctatc cgctcgagcc
     5521 ggaagcgccc gccgcggcct acatggccgg cgtgggtctc ctggcgcaga accgcccgcg
     5581 cgccgcggcg ccctacttcc agatcgtcct cgaccgctac gtggcgagcg cgatggattc
     5641 ctcgcgctcg gtgcggccga tcacgccgga gcgccaggag ctgatcgacg cggcgctctg
     5701 cctgctcctc tattcgtacc ggaccgcggg cgacctgggc caggtggccg gcgcgccgca
     5761 tctcttgctg cagaagatgc cgcccagcca caatccctgg cgcgcctacg cgctcctgat
     5821 cgacgccgac gccatggccg cccagtcgcg gcatcccgag gcgcaggcca cgctggagaa
     5881 gctgatccac gacttccccg accacccggt cggcgcgtcg gcggcgaagc tcctggcctg
     5941 gacctatgcg cagcaaggca aggacagcct cgcggtgcag acggaagaga agctggtgct
     6001 gcgctacggc gcgaccgacc aggtggtcgt ggccggcgcg ctgctcgaca tcgcccacga
     6061 tcgcttcaat cagaagcgct atcgcgaggc ggcggcgggc tacgaggact tcctgaagcg
     6121 cttccccgcg agccccaagc gccacctggc gaactaccag gccgggctcg cctacgtccg
     6181 cctgaaccgc ctgggcgacg cggtggatca ctgggaggcg atcgttcgcg acagctccaa
     6241 ggccccgatc gccgagaagg cgtgggcgcg cgcgggcgac ctctacttcc aggcgcagcg
     6301 ctacaacgac gcgaagcgct gctatcgcgg actcctcgag aacttcgcca cgaccgacgc
     6361 cgcggcgctc gccacgctcc ggatgggcca gtgcgagtac aacgccggca acgacgcggc
     6421 cgcgctcgag tccttctcgc aggcgatggc ccgcttcccg gacagcccct acgccaagga
     6481 ggcgaagcgc ggcaccgagc tcgcgctcta ccgcctgggc cagagcgaca agggaacggc
     6541 cgtgctcgcg cagctggtcg agcagtatcc gaacagcgcc tacgcggcgg acgcgctctt
     6601 ccagatcgcg aagaacgact atcaggcgaa gcgctacgac aaggcttcgg acggcttccg
     6661 ccgggtcgtg agccagttcc ccggctactc ggcggcggac caggcgcagt acctcctggc
     6721 ggacgcgctg gacaaggccg gcaagccggc cgaggcgcgg caggcctccg agcagttcct
     6781 cgccttcttc cccgagagcg acctgcgccc gacggtgcag ttccaggtcg ggctgcagca
     6841 gttccagaac aaggagtacg cgcccgccgc ggtggccttc acgcaggtgc tcgccgacag
     6901 cgcggcgccc gacgtgcgcg ccgccgcccg ctacaacctg gcgctctgcc agcgctcgct
     6961 cgggcagaac gacgaggcgc gcgcgacgct cgaggcgtac cggaacgagc gcccggccga
     7021 ttcccgagcc gaggaggtcg cctaccagct gggcgacatc catgacggcg cgggacgcta
     7081 tgccgaggcg cagtcggaat tcgagaaggc cctcgacggc aagccgagcg ccgcgctcga
     7141 gacggagctc cggttccgcg tcggccgctg cctcgaggag cagcagaaga tcgacgaggc
     7201 gacccgctgg tacatccgcg cgtcccaggc ggtggaccgg cggaacgcat accgcctctc
     7261 ggccgtcgcc cgcctggcgg cgatctacga gtccaagcgc gagttcggaa aggccatggc
     7321 ggcctatcag gacatcgcct cgtactcgaa ggaccgggag ctggcgcagg ccgcggccgg
     7381 ccgcgcgacc cagcttcgtt cgagccgcac gacccgctag tcccgaacac cgaaaggagc
     7441 gagacgacga tgttcgagaa tttcgactgg atccaggcca tgcggacgag tcccgtgatg
     7501 gtcgtcatcc tcttctgcag cgtgctcacc gcgggcttcg cgctggagcg cgcgctctac
     7561 tactggaagc ggcgcggcaa tcccgacgcc ctgctcgcgt cggcgctggt ggccatgcgt
     7621 tcgggcaacc gtgaggaagc ggtgcgggtg ctgtcgtcga atccccaccc gatgggcgcc
     7681 gtcgcggcgg agctggtgaa gaacctcgac cagccggaag gcgtgatcga ggagaagatg
     7741 cacatcgccc tgagcgagca gcgcatgcag ctcgaccgga atctcggctt catcggaacg
     7801 atgggcaaca cggcgccgct gatcggcctc ctcggaacgg tgtggggcat catgcgcgcg
     7861 ttccacgaca tggcgcggac cggatccgcc ggaccgtccg tggtggcggc gggcgtggcc
     7921 gaggcgctct tcacgacggc ggcgggactc tgcgtggccg tcccggcggt catcatctac
     7981 aaccatttcg ttcgccggat gggcgtcatg ctgaccgtcg ccgagaacaa cgcccgcacg
     8041 atccgcatga acgcgaccgc ctcctcgcac caggcccacc gggccgctta ggagatcatc
     8101 catggccggt cgccgatggc gcatgcgcaa tccggagtcg atcttcgagc cgaacatgac
     8161 tccgctcatc gacgtttgcc tggtgctcgt cgtgatcttg atggtcgcca cgccgatggc
     8221 gttccagtcg agcatcggcg tgcagaaggc ggccaaggcc ggcaaggcgg cggcgatcgc
     8281 ggccaagtcg gagcgggtgg agatctccat cgtctccgcg gacacggtga cggtgaaccg
     8341 gaccacggtg gtccggaagg acctgccgct cctgttgaag ccgctgctcc agctgagcaa
     8401 caccaagacg gtcatcgtcc actgcgcgga tacggtgccg catggcgcgt tcgtgggcgt
     8461 tctcgacgaa gccaagcagt gcggggccgc gcagatcgcg gtcgtgggag ggtgagatgc
     8521 ggagcttcac gccgcggcag acgctgatca ccaaggtcaa cgtcacgccg atcatcgacg
     8581 tcgctctggt gctcgtgatc atcctcttgg tcacggctcc gatgctggcc gtctccgacc
     8641 tgccggtgaa cctgccgcag gcccagacgc gcgagtcgga agacgagcgc aacctgagca
     8701 tcacgctgtc gtcctcgggc gagctggccc tcgacgaggc gcgggtgacc aaggaaaccc
     8761 tgaccgccgc gatccacgag cggctgcaga agaaagggga cgagaacgtg ctcgtcgtgg
     8821 tgcgcgcgga cgaggacgcc ccctattccg tgatccggga gctgctcgac gacgcgcgcg
     8881 cggcgggggc cacgcgcatc gccatcgcga cgcgccagaa gacggggacg ccccgatgac
     8941 cgccgccacc ctgcgcctcg atctcatgga gtgccatacc cgcacgcgcc gcaccatgat
     9001 cgcgagcgtg gtgctccacg cgctcctctt tctctggatc gccaccttca agacggccga
     9061 ggtcctgacg ccgccgatca ccgagatcac catgctctcg gccggcgacc tggccacgat
     9121 cgacgccggg cccgccgagc cggcgccgcg ggtcgtgccg cgtccgggcc tggcgccgcg
     9181 caaggtggac gtggaccagc gtttcgttcg cagcgcggac cgcgccgaca tcacgctcga
     9241 tccgcagagc ccgacctcga tcgccgacca gctctcggcc cgtctctccc ggctgaccca
     9301 ggaggagcgg cccgccgtgc ccggcacggt ggtcgccccg ccgagcgcgg tgttcggcag
     9361 cggccccgtg accgcgaagg gcatgggaac gggcacgccg gtggcgctct cccgtggcgg
     9421 cgccctgggc ggagcgggcg ctctgccgct gaatcgcggc gggggaggcg gcggctcggc
     9481 ggcttccgga ccggccacgc tgcccgccgt gaagaccgcc gccacggccc ccgcccaggt
     9541 gggtgacgcg accgcgcgcc gcacgatcgc gggcgcctcg gtcgcgggac cgatcgccga
     9601 ccgggcggtg ctccagacgg tcgtgccgga atatccggaa tgggccaaga aggaagcagt
     9661 cgagggttcg gtgacgctct acttcgtggt gcgctccgac ggcaccgtga aggagaacgt
     9721 cgtgatccag aagacggccg gcttcggcga cttcgacgag aatgcgcgga cggcgctccg
     9781 gggctggctg ttcgagccgc tccgcggcgg acggacgggc gagcagtggg gaacgatcac
     9841 cttccacttc cggcttcgcg acgccggctg atcgaggaga cgcgatgaac ctgcgaacct
     9901 atcgtgacac cgtcgcggct gccgccgcgg ccgcgctgac gctcctcttc gtcgcgcacc
     9961 acgcgaccgc cggaacgacg ggaacggcga agccctcggt caccgtcggc acggtgagcg
    10021 tcgtgacgcc gacgcccgca gggccggaga cgtcggccgc cgtgccgggc acgcccgccg
    10081 cgaaagcgcc ggccgcgaag ccggcggcgg tgacgccgaa gcgcaaggct gcgaagaaga
    10141 gcgcggccaa gcccgctccc gctccgagcg aggccgcgaa cgcggcggac gcgaagggtt
    10201 ccgttggcgc gacggcgggc gcggcggatg cgaagggcgg ggctccgaac gcggcgggcg
    10261 cgacgccggc gggcaacgcg acgccggcgg gcaacgcgac gccgaatgcc gcggctgcgg
    10321 acgccaagga cggcgagaag ggctacacca tcaagggcgg cgccgagcgc accgacctcc
    10381 gctcgctcac gatcgagggc gaggaccggg tgcacgtgga gatcgagcgg cccccgctcg
    10441 cgctgcagct cgaccccgcg aaggtgaacg ggctggactt cgggacggcg caggacgtgc
    10501 tggaccgcac gccgcccgat ctcacgacgc agtacctcgc ggtctccgcg aacgagcgct
    10561 ccccctacac ggggcgtccc tggctccggc agttcgcgag cgggtcggtg gcgcgcttcc
    10621 agcccaacgt gcgcggcacc gagcggtgga agctgacggt ggccgactcg cacgggcaga
    10681 cggtggcgca gttcggcggc aagggcgagg cccccaagga gatcgtctgg gacggccgct
    10741 ccctctcggg cgccccggtc acgccgggca tcacttattc ctacgtcttc gaggcggtgg
    10801 acaaggcggg caacaagcgg aacttcgtgg gccaggggtt caaggtatcg gcctatcggc
    10861 tcgactcccc tctcggtccc accctggtct tctcggccca gacgctgcag gagggtgccg
    10921 tcgcgacggc ctacgggccg accggcgccg ccacgcggcc cgcgccgccg atcgttctgg
    10981 aagcggcgag cacgctcaac cagtcgccgc gcgtgtcgca gccgatccgc gtgaacgtga
    11041 ccggccgaac ctacgaatac gcgaacgcga ccgccaagca ggtcgcggcc tggatcgcgg
    11101 atctcacgat cggcgacccc gcgcgcgtgc aggcggtggc ccaggtcgag ccggacgccc
    11161 ccgagggagg cacggtgcgc atcgcgctcg atggcgtcgg ggtggcgggc aatgcggtgc
    11221 cgacgatgaa ggcgccgaag gtcgagaaga gcggaaagaa gtcgaagaag ggttaagaag
    11281 cgcgcagcgc tggacggtct ctcacagggc cgcgggctca ggctcgcggc ccttctcctt
    11341 gagtggcagt taagttatat gttaagtaca attgaagaca gcaggagcga tggtccgcgc
    11401 gcacgggatg ctgtacaaac agggcgccgg ttcccggttc gcgcaccgat tcagtcgcgc
    11461 gatcctaaga aaagagtagc tgtgcgattt tcttgttgac gtacttgtgc ttccctcctc
    11521 gaaaccttgc tcattcccct atggatccgc ccgccagacg ttctagaagc ccgcgtgtgc
    11581 cacgatcgca accttcttga gagaaacgac tcggcacgcc tcgacgtctc gttgacctgc
    11641 cgggccgttg cccaattctt cttgtgatac aaagaaagta cccccgtcga cgaccggccc
    11701 ccgccggaaa cgccccacgc gccccgagga gagaacgacc atgcgcgact acatcttgac
    11761 tcacctcagc gacacaaagc tccttcgtga attgacggtc ctggcttccc gagatcgaga
    11821 aacgacggcc ctgctcctcg cgcacatcgc tgaggtcgat gcgcgccgtg cttacgttcc
    11881 ggagggatac tcctccatgt tttcgtactg cgtggagtgt cttcgactct ccgaggacgc
    11941 agccgcgaag cgcatccagg cggcacgagc ggctcggcgc gttcccgtcc tcttcgcagc
    12001 cctggccacc gggaagctgc acctctccgc ggtgtggctt atggcacccc acatcactcc
    12061 ggaaaatgcc gaggagctga tcgaggccgc cacgcatcgg cgcaagtccg agatcgaggc
    12121 gcttctcgct cggacctttc cggcgttcgg agcccccctg ccggcagcgc gcatcacgcc
    12181 ggttggttcg ggaatgcctc gagcctcgca aggaggcggg tctctgttcg acgaacatgc
    12241 cccagggcat gttggaaacg gagacggggc attgctgatg caacgtgccc cggggcatgt
    12301 cggtgacggg gacggcgacg tgctcgagca acatgcccca gggcatgttg aaaacggcga
    12361 cgccacgctc cggcaacatg ccccagggca tgttgcgacg gacgtggtcc aggcgctgca
    12421 tgtcgatgcg gcctcgcagg agcgctacct cgtccgggtg acgatcgccc ggagcacgca
    12481 cgacaagctt cgccacgcac aggccttgct cagccattcc gttccatccc aggacgtagc
    12541 ccaggtcctc gaccgggcgc tggatgccct gatcgcgcgg ctcgagaggc ggaagctcgg
    12601 tgccgctcgg ccggcgaaga gtgcggtcca gtgcgagacg ggggttcctg gccctgctcg
    12661 agcccaccga agcgccagcc gctacatacc ggctccggtt cggcgtgccg tgtgggagcg
    12721 tgacggaggg cggtgcacct ttgtgggcgc cggccggcat cgctgtgaaa gccgccgttt
    12781 cctcgagttc gatcatgccg agccgttcgc gcgcggtggc acagccaccg tggaggggct
    12841 gcgccttcgg tgccgagcac acaatcaatt cgcggcggag cgcgcctttg gggccgagtt
    12901 catgcgcctg agacggcatg aggctcgggt cgcgcgccag aacgcggcgg cccagcgcgc
    12961 ggcgatggcc gagagagcag cggcggatca gcgggaggcg gcacgcaaag agcagatgaa
    13021 ggatctggag gcaggactgc gcggcctggg atgtcgagcg gacgaggcgc gtcgcgctgc
    13081 ggactatgct atggaaatcg agggcgccac ctttgaagat cgcctccgcg ccgcactggc
    13141 gttccggtcg cgccgtactg tgacgcgctc tagggtcacc gaggcatgcg aggttcgtga
    13201 ccggagggcg ctctatggcg cacccgcatg ggcgcggccg gtctacaatg ccccgcatgc
    13261 ggaccgcgag cctcattatc gccgtgcttg gagccgttct cgcggtccag gcccatgcca
    13321 tgccccagtc ggaagactcc tcgcgagcga ccgagccgcg ggtggcggtc caaaatggcg
    13381 tcgcctggtt ctacgggcat cggttcgagg cgccctttac cctggaatac tgcgatgacg
    13441 gtctcgccat caatggatcg tttctgcccc gcgaagtacg cctctttcca aaatctcgac
    13501 ctgggcacgg cgacacgctc agggcggagt tgagcgcccg ggccctcgag atcctgcgcg
    13561 acgctcaaag aaatcatgtg ccgcgggacg aagcgttcgc ccagatcgag cagttgtacc
    13621 gctcatgccc cctggtgaaa cgggccgagc tgcggggata ctcgctgcgg acctcctaca
    13681 aggaccgttc gggggattac atcctccagc tcgtggatcc cgaaagggtc gccatgcgcg
    13741 acatggcact cgaagaatcg tccctgaaag agcgcgggac ccagtcgaag cacctgcttc
    13801 aactcaagga tcatctcgag tcggccggct cgatcctctg gttcgattcg ccgggttacg
    13861 tcttgctccc taaggacgac tcggcccgaa tcgacgccat tctccaacgc atgcagcaaa
    13921 aggagaagat caccaaggag gaaggtgcct ttctgcaatc actgatcccg gcctcactcg
    13981 gtgagctgag ccgcatggcg gaacagccca agcgtctcct gaccctcttc ccccacccgg
    14041 aggcccgatg accgacgcgc ccaaggatcc tgagcggcgg gccacgttca tcctggcccg
    14101 cggcgagaag tggctgctgc cgcgcctggc ggcgtcgctc ccgcgatgga tggtccccga
    14161 ccacctgacg gcgatcggca ttctcggcgc caccctgatc gcgctgggtt acatcctctc
    14221 cacgcgcgac cggcactggc tctggctctc gaacgcgggg ctgatcgtga actggttcgg
    14281 cgacagcctg gatgggacgc tggctcgcgt ccggaagcac gagcgcccgc ggtacgggtt
    14341 ctacctggac cacctcaccg acgcctattc cacgaccgcg gtcggcctgg ggctgggctt
    14401 ttccccttac atgctcctct cggtcgggct cgcgatcgtg atcgcctacc tgatcctctc
    14461 gatcaacgtc tacctggaga cgcacgtctt cggcgtcttc cgatacggct acggcgtcct
    14521 ggggcccacg gaagctcgcg gggtgctgtt cctgctcaat atcgtcgccc tggtcacggg
    14581 gccgatccct ttcaccatcc acggcatccg tttcaccgcc ttcgacgtgg ccggagcctc
    14641 cgccgccctg tcgatgggag gcctcctgct ccgccgggtc gtccggaatc taggtgagct
    14701 ggcgaggatg gaacctccga ggtcgtaagg ctcctgtttc gaggagcttc ccgggctggg
    14761 cgattttccc cctcggccga atcctggttt ccgaatccgc cacgctgcca tagacttatc
    14821 ggatggcccg aacgggtgaa ccaaccggta cgcgggttct ccgcgcactt cgaagggcag
    14881 cgacacggca gacggtccgg cgcgagttcg aacgccagac cggcccctcc gacctggaac
    14941 ggagcgtccg cgaatactgc ggcgacaccc ggatcgtcat cgcctcaaac cgcgagccct
    15001 accagcacgt ccatggggcg cacgggatcg aagtgatccg gagccccggc gggctcgcct
    15061 ccgcgctcga ctcggtcgcc cgcgccacga acgcgacctg ggtggcgcaa gccaccggcg
    15121 acgccgaccg ggagacagcc gacgctcagg gccgggtcgc gatgcccccc ggatcgaacc
    15181 gctacaccct gcagcgcgtc tggatcgagc ccgaggcgca tgccgacggc tacaaccgct
    15241 tcgcgaacgg ctgcctctgg cctctctgcc acgtcgtcta cgtgcgcccg cactttctct
    15301 gggacgagtg gcgcgactac cgcgaggtga acgagcagtt cgccgacgcc atcgccgcca
    15361 gcgtgggacc agagccggcg ctggtgctgt tacaggacta tcacctggcg ctctgcgcgt
    15421 cccggctgcg ccagaagcgg cccgacctgt cgatcgtcct cttctggcac atcccctggc
    15481 ccaatcccga ggtgttccgc atccttccct ggaagctcga agtcctggaa gggctgttgg
    15541 cgtgcgacct cctgggattc cacattcccc atcacgcgat gaacttcgtg gacaccgtcg
    15601 ccaccgagct ggaggcgcac atcgaccggg agcgatcggc ggtgcggcgc cgcggccacc
    15661 gcacgttcgt gcgctcgtac gcgatcggcc ccgacgtggc ggagatctcg atggcggcgg
    15721 ccgatcccac gacccggcgc gaggcggagc gggtgcgcga aaccctcggg ctgggcggcg
    15781 cccgcgtcat cctgggcgtg gaccggctgg actacacgaa aggcgtgccg gagcgcctgg
    15841 atgccttcgg ccgcttcctc gagctctatc ccgaccggcg cgaggacgtc gtcatggtgc
    15901 agatcggcgt gccgacgcgc gtgaatctgc cggagtacca ggcgctgatg gcggacgtgg
    15961 agcagcgcgt ggagcggctc aacgccgcgt acggcaggaa cggacggaac gtcgtgcacc
    16021 tgatcacgcg gaatctcgac ttccgggagc tgatccccta ttacgtattg gcggatgtga
    16081 tggccgtgac ctcgctgcac gacgggatga acctggtggc caaggaatat gtcgcggccc
    16141 gccccggcgg cgacggcgtt ctgatcctga gccccttcac cggagcctcg cgcgagttgg
    16201 agcacgcgat ccaggtctca ccctacgacc gcgagggcct cgcggtggcg ttccagcggg
    16261 cgctcacgct gagcccggcc gagcgcgccg accgcatgcg ctcgctgcgg gaggtcgtgg
    16321 ccgcgcagaa cgtgtacgac tgggcgcgca agctgatccg cgacgtgcgc cggctgcatc
    16381 tgctccccgg gatgcggccg gcggggaggc gatgacggcg gcgcggcgcc gcgcgccgcg
    16441 gcgcctgctg gcggacggag cgtgggagga tgcggaagcc tcgctcgcgc gcgcggcgcg
    16501 cgccggacgc ccggcgctcg tcgcgctcga tttcgacggc acgctcgcgc ccatcgtcag
    16561 ccgccccgac cgggtgcggg tgccctccgc cacgctgcga tcgctccggg acgcgtcgaa
    16621 gctcccgtgg ctccggatcg cggctttgag cgcgcgtccg atgcgcgatc tggcccgcta
    16681 cttgccggta accggaattc tgcgcgtcgc gcagtacggc ctcgaaggcg gcgacgcgct
    16741 cgatccgaag cggctccgcg cgatccgcgc cggcgtcgac gccctgcacg ggtggatcga
    16801 gccgctcgcc gcggccgcgc ccggcgcgtg gatcgaggac aagcggctga cgatcgcggt
    16861 ccactatcgc gacgtcgcgc cccggcaccg cggcgccctg gtgcgcaggc ttcgtacgct
    16921 ggccgcgcgc gccgaaaggc tcggctttca cgtggagcgc ggccggcgcg tgctggactt
    16981 cgtgccggcg gggtacgata agggacgggc gctccgcatg ctgcgccgcc gggttcgccc
    17041 ggcggtcacg ttctacttcg gcgacaccgc cggcgacgag ccggcgttcg ccgcgctcgg
    17101 ccggcgcgat tttcccgtgc gcgtcgggac cgggcccacc gcggctccgt atcgcgttcg
    17161 cggacccgag gaggtcgcgc gcttcctgcg cgcgctcgtc tcgaaaagga ggtgacacga
    17221 tgagctggga ttcggtcata ccccccgatc tggccctgca gctccacgac acggtcgtgt
    17281 cggtcgtcgg cggcatcatc ctcgtgctcg tcgcgggcgc gatcgcgttc ctcggctggc
    17341 tcgtcgccac cctcgtcggc cgcgcggcgc agtggatcct ctcccacacc ggcgtggacg
    17401 cctcgacgca gaagctctcg cccggcgagg gccggcgcgt ggagctctac cccagccggc
    17461 tcgtggggta cgccctcttc tggaccatct tcttgagcgc ctgcatcgtc gcgctccgcg
    17521 tcgtcgggct ggatctcgcc ccgtccatcg cggcgcggct ccaggacgtc gtgccgcgcg
    17581 tcctgacgtc ggccgtcgtg ctcattctgg gcattccgct cgcgctggcg ggaagccgca
    17641 tcctgaacgc gctgctcgcg ccctccgggt tgaagcccaa ccggatccgc tcccaggcgg
    17701 tgtcggcgct cctcatcgga ttcgtcgtgc tggtcgctct ggagcagctg gggctggcgg
    17761 cccagctggt gatcgccatc ggcatcaccg tcgtcgcggc cgcggggctc gcgctcgcgc
    17821 tggccttcgg cctcggatgc cgcgacctcg cgcgcgatct catcgtcgaa tacctgcgcg
    17881 ccggcgagga aggaccgcac ccgggccggc catgacgcgg caggggcaag gacacggggc
    17941 ggccgcgacc gggggccgcc catgagcgac gatcgcttcg cgttctgctc cgtcgcccat
    18001 ctcgaggcgc cctccggcat cgtcgcgctc agcatggacg cgctgcgggc cggcatcgag
    18061 agagcgcccg acgattcgct cttccagcac gtcacctgcg tggcgatgcg ctatccgcac
    18121 gcgcgcgacc tgccgagcaa cgatttcgcg cgctgggtgg gcacgtcgct gcaggccccc
    18181 gacgtcgccg agcgcctggc ctacgccggg gccgccacgg cacagccgat ccgggaactt
    18241 cgcgcgtcgc tcctctccgt tctcgattcc gtgccgcagc cggagcgcag tcgcgaggcg
    18301 cctccggagg cggcgttcca tttcgtaggc gtgcggtcgg tccccgttcc gctcggcatc
    18361 gaggcctccg atccggtgca ggcgatcgac gtctggcacc gtctcgattc cgcggccctc
    18421 ttcttccacg tgatcgaggc gccggtgttc ggcgacgccg cgaattcctt cccccactgg
    18481 ctgcgccgcc acggcgccga cgccctggcc atccaggccg agcaggcggt ggccgcgggc
    18541 cggccgatcg cccggctccg gaaggacctc ggggcgcgct ggcgccgctc gcagatcgga
    18601 cggcgtctcg cggagcgcgc gggggcgccg gaggaggagc gcctgcgcga ggcgcgcgcc
    18661 gcgatggcgc gtctcgcggg ccgcctccgc gggggagccg gcaagcgccg gaacgacggt
    18721 ggtcccgagg agcggccatg agcgccatcc gcgacgccgc gcgcgtcccc tacgagcacc
    18781 cgcccgccga gcactggctc gcgcgctacg agccggtcgt gggcgccggc acgctgcgcg
    18841 cgctggaggc gctggcgcgg aagctccgcg gccaccgcat cgtgatggtg aacacgacca
    18901 agaccggcgg cggggtcgcc gagatcctcc accgcgtggt cgtgatcctg aacgagctgg
    18961 gggtgccgac gacctgggag gtgatggagg gggacgatcg tttcttcggc gtcaccaagc
    19021 gcatgcacaa cgcgctgcat ggccacgtgg agccgctcac cgagcaggac cgggagatct
    19081 accacgagcg gacgcggctc gaggccgatc gcctcgccct ggacggcgac ctgatcttca
    19141 tccacgatcc gcagccggcc gccctgatcg agcaccgccg gcagcccggc cagaagtggg
    19201 tctggcgatg ccacatcgat ctgtcgcgcc gcgatcccgc gtactgggac ttcctccagc
    19261 cctacgtctc gcgctacgac gcgtcgatct tctcgcacat cgcgttcgtt ccgccgatca
    19321 ccatccccgc ctacctcgtg ccgccgtcga tcgatccctt cgcgacgaag aaccgcgacc
    19381 tgagcgagca cgagatcgag ggcgtgctcg agcggctcga cctctccccc ggccccggct
    19441 ggatcacgca ggtgtctcgg ttcgaccgga tcaaggaccc gatcggcgtc atcgacgcct
    19501 atcgtctcgt gcgggctcgt cgcccgaacg cgcgtctgct gctggccggt ggaggcgccg
    19561 acgacgatcc cgagggcgcg gaggtgctcg ccgaggtgcg cgcgcgcgcg accgcggcgc
    19621 ccggcgtgac ggtgctggag ctgccccccg ggtccgatct cgagatcaac gccatccagc
    19681 gtgcttcgac ggtggtgctg cagaagtcgc tgcgcgaggg attcgcgctc accgtgagcg
    19741 aggccttgtg gaagcggcgc gccgtcgtgg cgagcgcggt cggcgggatc ccgttacagg
    19801 tcctgcacga gcagaccggc ctcctggtcc actccatcga ggggaccgcc ttccagacga
    19861 tccgcctgct ggacaacgcg gagctccgcc gctcgctggg cgacgagggc cgccagcacg
    19921 tgcgggacaa cttcctgcac acgcgggagg ttcgggacta cctggcggtc ttcgcgtccc
    19981 tggtgtgagg cgcggggttt cggagctcga cgcgccagat ccatacgtcg ccggagtcct
    20041 gccggcgcga ttccagagag cggagcatct cgtcgagctt gcggtgcgtg gtcgcgaagg
    20101 cgcgctgctt ctcgacgcct ccgatgcctc ggacgttctc cgcaaccggg atcgaaacca
    20161 accggatcgg gcggcccgga atggccgaag ggacccgcgc gatcatttct tccacctcgt
    20221 ggcgtgggga gcgcgacccg atcaccacga ttcgctcccg cccgccgaag ctcgtgaccg
    20281 tccagtaggc caggctgtcg cccgccgggc ccggaagcag atggcggacc cgagacgaaa
    20341 gcggattcga cgcctcgaga cccggaatgg gaaagagccc gtacacgtcg cccgcctcgt
    20401 cctcatcgag aacgtaggtg tgcatcggct gttcggagct gacctccagg aagagtcggt
    20461 ctccgggagc gatggtcgcg cccgaaggga gaggctcgtc gcgggcgccg agatgccgga
    20521 acagggatgc gctcgcctga agcggcttcg ccgcccacga cgcattcccc gacgccgatg
    20581 tcccggccgg ccgggcgccg gccgggcgta cgtcccgatg cgctgagcgc gatccgatga
    20641 gccatgaaac ggccacgaga ccagcgaccg cgatgacggc caccccgacg gaccgcgcca
    20701 cgagccggcg agagccggtt cctcgcgcct ccagggctgt ggtcaaggcc cgccccatcg
    20761 cccccgccga cagatagcgc cggctcggct cgggggacag cgcgcgatcc acgacctgaa
    20821 cgaaatccgg aggaaggtcg ggacggcgat cgctgagggg cacacccgcg ccgcgctcgt
    20881 ggagccgcaa cagctcggac acgcttctgc cttcgaccgg atagcggcag gtgaccaatc
    20941 ggtagaggag caccccgagg ccgtacacgt cgcttcgagc cgtcgcgggc gcgccccgaa
    21001 gccgctcggg tgccatggcg accggcgtgc cggacgcttc ctcgacgcgc tgttccatcc
    21061 ccgcggccga acgaagctcc gccacgcatc cgaagtccat caggagaatg cgaccaccct
    21121 gctcgcgcat cacattgctc ggcttcacgt cgcagtggac gagtccgctc gcatggaccg
    21181 ccgcgagggc gcggcagaga tccaggccga tccgggccgc ctctcccgcc tcgagagcgc
    21241 cttcgcggcg aatgtattcc tcgagagagc acccccggat gaaatcggtc cagatcccca
    21301 tgcggccgtc gtgctcgccc accccgtgga cggcgaggac gttggggtga cggacctgcg
    21361 ccagcttccg ggcttcgccc tgaaagcgct cgcgcgcggc gggactccat gaagcgtccg
    21421 gcttcagtag cttgagggcc acctcggttc gaagcccggg gtcgaaggca cgatagacct
    21481 ctccgcccgc gccttctccg acgcgctcga gcacctggag cggcccccag gcgaacagca
    21541 ccgggcccgg ctcaggcgcc ggcggaacgg cggtctcggt cggcgcaccg agcaaccggg
    21601 tcgagcgatc gtcggccacc ggcatcagcg accgaatccg gtgtccaagc acgttcgctc
    21661 tcatgatccc ctccggacgg aacaacgcct ccggggaccc gatgcgcaca aaaggaatta
    21721 cacctgcgcc tccacgacgg cgacaccgtg gcgcatgccc gcggcggcgc ccgtctgacc
    21781 ggccgcacgc gccgaccacg cgctcgcctg caccgcgccg atcccctgga gcgggcatgc
    21841 cagctccttc gcgctgcccg aatccagtcc ccgggaccat gccagggtct tcgttcgatc
    21901 caggtcgagc gtgccgtcct cccgaatccg gccgacctga ttggccggaa gctccagggc
    21961 gaccgtgcac ttgttcaatc gcccgtccgc ccgaaccagg aacgaattgg catgcgccgc
    22021 gtagcagacc ggatcccccg cgttggcttg ggccagcttc agtcccaggc gcgccgcctc
    22081 ctccctgagg cgggccagga tg
//
LOCUS       DASSLZ010000021         5229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_515318, whole genome shotgun sequence.
ACCESSION   DASSLZ010000021 DASSLZ010000000
VERSION     DASSLZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515318"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /gene="lpxD"
                     /locus_tag="VFM00_00730"
     CDS             <1..860
                     /gene="lpxD"
                     /locus_tag="VFM00_00730"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HET7496803.1"
                     /translation="AEDHRDLGKPLIQNAHPYLAFLKAVRFFQGEEARPEPGIHPSAV
                     IAPDACVDATASIGPHVVIESGARIGPLAWIGPGCYIGAGASIGPETRLHPNVTLREE
                     CMLGARVIVHPGAVIGSDGFGFVRDGDIYRKLPQVGNVVVEDDVELGACVTIDRATMG
                     TTRIGQGSKIDNLVQIAHNVQVGRNCIIVAQVGISGSTSVGDNVVLAGQVGIVGHIEI
                     GAGAQVGAKSGVSKSVKPGERIFGYPALPVSQAKRIEASIRHLPELIQTVRALRRRIE
                     ELEGPKPSS"
     gene            857..2191
                     /locus_tag="VFM00_00735"
     CDS             857..2191
                     /locus_tag="VFM00_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HET7496804.1"
                     /translation="MSAPNQRTLGRTVAYDGVGLHTGIPCHVEFRPAPPNTGIRFVRL
                     DLPGTPEIPVAPRYARADTDQMRRTILKNGDAEVHTVEHILAAAAGLEIDNMVIALNA
                     QEPPEPQDGSARPYVALFQGAGIVEQPAARQYFRVTEPVEYEEGGVILVALPHDGLRV
                     TFTIEYENAYRGTQYATYDITPETFVKEIAPARTFVLERDVESLRARGMIQGGTLQNA
                     VVVQPDGIMNREPLRFEDEFVRHKILDFLGDLFLLGRPARGHFLSIKSGHATNVRFVQ
                     RLIQDQEPGSLIRGIVGNGDHGPIQLDIMAIQKIMPHRYPLLLIDRILSLEENRVVGI
                     KNVTINEPFFAGHFPGHPIMPAVLIIEAMAQCGGVLLLNTVERPKDKLVYFMGIDNAK
                     FRKPVRPGDQLRFELTLLRLKQRICKMEGKAYVHGDLVAEAELLSSIVERNP"
     gene            2244..3038
                     /gene="lpxA"
                     /locus_tag="VFM00_00740"
     CDS             2244..3038
                     /gene="lpxA"
                     /locus_tag="VFM00_00740"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET7496805.1"
                     /translation="MPDSERARPSIHPTAVVARGAELGPGVVVGPFAYVGPRVVLGAG
                     TRVGLHAIIEGCTVVGPGCQIHHGAVIGNIPQDLKFRGDPTHVKIGSETTIREYATIN
                     AATGEGETTVIGDRVLLMAYAHVAHNCSIGNGVILANCVNLAGHVRVDDFATIGGMTP
                     VHQFVAIGTQAFVGGASRVAQDVPPYVKAAGNPLRACGLNSIGMERRGISADVRLEMK
                     RAYRILYRSQLNVGPALDRILRELKPYPEIHEFVEFVRSSERGIAR"
     gene            3061..4050
                     /locus_tag="VFM00_00745"
     CDS             3061..4050
                     /locus_tag="VFM00_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7496806.1"
                     /translation="MRTLRAGVAGLGHLGTMHARAWQRIEGVELVGGFDPDPAARARA
                     QEALGITMHDSLAALLSGLDLLSIAAPTPAHHDLAKASLARGIATLVEKPMAATPAEG
                     EGILALARAQGAVLAVGQIERMNPAVRAALPHLKHPRFIEAHRLAPPVPRGTDVDVIL
                     DLMIHDLDLALLATRSRVVDVQAVGVPVVTPHIDIANARITFESGCVANMTASRISRE
                     KIRKIRFFEASEYLSIDCLHRTVEAFALEPAGPENPDAHWLARIRPLAIAVSSDDPLE
                     TELRHFTALRADPARSWAESETALEALRVADRVRRQVEHRPVRSEPTGPSVPV"
     gene            4098..>5229
                     /gene="lpxB"
                     /locus_tag="VFM00_00750"
     CDS             4098..>5229
                     /gene="lpxB"
                     /locus_tag="VFM00_00750"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HET7496807.1"
                     /translation="MGAALLAALRARGVRVEAAGLAGPALREAGVLAAAPMESVAVMG
                     FAEVIRHLPAIRRARAAVLALLEQDPEAIFLPIDSPGLNLGLARAAHRMGRRVVYYVL
                     PQVWAWGYGRVRRIREDVDLMLLLFRFEEAIAAREGIAARWVGHPAGALAPPPAARRD
                     ARSALGIEDDATLIALLPGSRVGEVRRHLAPMLDAAARLAGAWEKPVRVAVSDAGPVA
                     GTAARGDAAPLWNAVRPLRVAGTSAPLLAAADLALVASGTATLEAAVIGAPFVIVYRT
                     GRLNYEIARRLVRLPRVGLANIVVGADAAPECIQDRATGIEIARAAAPLLVESARREA
                     QRAAFAPLPELLGGGGSADRAAEALIEFVERGKVPAAAGAARE"
BASE COUNT          747 a         1896 c         1840 g          746 t
ORIGIN      
        1 tggccgagga tcatcgcgac ctgggcaagc cgctcatcca gaacgcccat ccctatctcg
       61 ccttcctgaa agcggtccgc ttcttccagg gggaggaggc ccgccccgag ccgggcatcc
      121 atccctccgc cgtcatcgcg cccgacgcct gcgtcgacgc caccgcctcg atcggtcccc
      181 atgtcgtgat cgaatcgggc gcccgcatcg gcccgctcgc ctggatcggc cccggctgct
      241 acatcggcgc cggcgcctcg atcggcccgg agacgcggct ccatcccaac gtgaccctcc
      301 gcgaggagtg catgctgggc gcgcgcgtca tcgtccatcc cggcgccgtg atcggcagcg
      361 acgggttcgg cttcgtgcgc gacggcgaca tctaccggaa gctcccgcag gtcgggaacg
      421 tggtcgtcga ggacgacgtg gagctgggcg cgtgcgtcac gatcgaccgc gcgaccatgg
      481 gcaccacgcg gatcgggcag ggaagcaaga tcgacaacct ggtccagatc gcccacaacg
      541 tgcaggtcgg acggaactgc atcatcgtgg cgcaggtcgg catctccggg agcacctcgg
      601 tcggggacaa cgtggtcctg gccggacagg tcggcatcgt ggggcacatc gagatcgggg
      661 cgggggccca ggtgggcgcc aagtcgggcg tctccaagtc ggtgaagccg ggggagcgga
      721 tcttcggcta tccggcgctc cccgtgagcc aggcgaagcg catcgaagcg tcgatccggc
      781 acctccccga gctgatccag accgtccgcg ccctgaggcg ccggatcgag gagctggaag
      841 ggccgaagcc ctcctcgtga gcgcgccgaa ccagcggacc ttgggacgca ccgtcgccta
      901 cgacggcgtc gggctgcaca cgggcatccc ctgccacgtc gaattccgcc ccgcgccccc
      961 gaacaccggc atccgcttcg tacgcctcga tcttcccggc acgcccgaga tcccggtcgc
     1021 gccgcgctac gcgcgggccg acaccgacca gatgcgccgc accatcttga agaacgggga
     1081 cgcggaagtg catacggtcg agcacatcct cgccgccgcc gcgggcctcg agatcgacaa
     1141 catggtgatc gcgctgaacg cgcaggagcc gcccgagccg caggacggga gcgcgcgtcc
     1201 ctatgtcgcg ctcttccagg gcgccgggat cgtggagcag ccggccgcgc gccagtattt
     1261 ccgcgtgacc gagccggtgg agtacgaaga ggggggcgtg attctcgtgg cgcttccgca
     1321 cgacggcctc cgcgtcacgt tcaccatcga gtacgagaac gcctaccgcg gcacgcagta
     1381 cgccacgtac gacatcaccc ctgagacgtt cgtgaaggag atcgcgcccg cccgcacctt
     1441 cgtgctcgag cgcgacgtgg agagcctccg cgcgcgcggc atgatccagg ggggaacgct
     1501 ccagaacgcc gtcgtggtgc agcccgacgg gatcatgaac cgggagccgc tccgcttcga
     1561 ggacgagttc gtccggcaca agatcctcga cttcctcggg gacctcttcc tgctgggccg
     1621 cccggcgcgg gggcacttcc tttcgatcaa gtcggggcac gccaccaacg tccgcttcgt
     1681 gcagcgcctg atccaggatc aggagccggg gagcctgatc cgcggtatcg tggggaacgg
     1741 cgaccacggg ccgatccagc tcgacatcat ggcgatccag aagatcatgc cgcaccgcta
     1801 tccgctcctc ctcatcgacc gcatcctgag cctcgaggag aaccgggtgg tgggcatcaa
     1861 gaacgtcacc atcaacgaac cgttcttcgc cgggcacttc ccggggcatc cgatcatgcc
     1921 cgcggtgctc atcatcgaag cgatggcgca gtgcggcggg gtgctcctcc tgaacacggt
     1981 ggagcggccc aaggacaagc tcgtctactt catgggaatc gacaatgcga agttccggaa
     2041 gccggtgcgg cccggggacc agctgcgctt cgagctgacc ctgctccggc tgaagcagcg
     2101 catctgcaag atggagggaa aggcgtacgt ccatggagac ctcgtcgcgg aagcagagct
     2161 cctttccagc attgtcgagc gcaatccctg aagccgaccg gctgacgggg acgtgggccc
     2221 gcgtcgcggg cgccgtcgtg ccgctgcccg attcggagcg ggctcgaccc tcgatccatc
     2281 cgacggccgt cgtggcgcgc ggggcggagc tgggtcccgg cgtcgtggtg ggcccgttcg
     2341 cctacgtcgg gccccgcgtg gtcctcggcg cgggaacgcg cgtgggcctg cacgcgatca
     2401 tcgaggggtg cacggtggtc ggtcccggct gccagatcca tcacggcgcc gtgatcggga
     2461 acatccccca ggacctgaaa ttccggggcg atccgacgca cgtgaagatc ggatcggaga
     2521 ccaccatccg tgagtacgcg acgatcaacg cggcgaccgg ggaaggggag accacggtga
     2581 tcggcgatcg cgtgctcctc atggcgtacg cccacgtcgc ccacaactgc agcatcggca
     2641 acggcgtgat cctggcgaac tgcgtgaacc tggcggggca cgtgcgcgtc gacgacttcg
     2701 cgacgatcgg cggcatgacg cccgtgcatc agttcgtggc gatcggcacc caggccttcg
     2761 tcggcggcgc ttcgcgcgtg gcgcaggacg tgccgcccta cgtgaaggcg gcgggcaatc
     2821 cgctccgggc ctgcgggctg aacagcatcg gcatggagcg ccgcggcatc tccgcggacg
     2881 tgcgtctgga gatgaagcgc gcctaccgga tcctctaccg ctcgcagctc aacgtgggtc
     2941 ccgcgctcga ccggatcctg cgcgagttga agccctatcc cgagatccac gaattcgtcg
     3001 agttcgtgcg ctcctccgag cggggcatcg cgcgttgacc gccccggcgg gagcgccccg
     3061 gtgagaacgc tccgcgcggg cgtggcgggc ctggggcacc tgggcacgat gcacgcccgg
     3121 gcctggcagc ggatcgaggg cgtggagctg gtcggcgggt tcgatcccga ccccgcggcc
     3181 cgggcgcgcg cccaggaagc gctcgggatc accatgcacg actccctggc cgcgctcctt
     3241 tcgggactcg atctcctctc gatcgccgcg ccgacgccgg cgcatcacga tctggccaag
     3301 gcgtcgctgg cgcgcggcat cgccaccctg gtcgagaagc cgatggccgc cacccccgcc
     3361 gaaggcgagg ggatcctcgc actggcccgg gcccagggcg ccgtgctcgc cgtggggcag
     3421 atcgagcgga tgaaccccgc cgtgcgcgcg gcgctgccgc atctcaagca cccgcgcttc
     3481 atcgaggcgc accgcctggc gcctcccgtt ccgcgcggca ccgacgtgga cgtgatcctg
     3541 gatctcatga tccacgacct cgacctggcg ctcctggcca cgcgctcgcg cgtcgtggac
     3601 gtgcaggcgg tcggggtccc ggtggtcacg ccccacatcg acatcgcgaa cgcgcggatc
     3661 accttcgaga gcgggtgcgt cgcgaacatg acggcgagcc ggatctcgag ggagaagatc
     3721 cggaagatcc gcttcttcga agcgagcgag tatctttcga tcgactgcct gcaccggacg
     3781 gtggaggcgt tcgcgctcga gcccgccggg ccggagaatc ccgacgcgca ctggctcgcg
     3841 cgcatccgcc cgctcgccat cgcggtctcc agcgacgatc cgctggagac cgagctccgg
     3901 cacttcacgg cccttcgcgc cgatcccgcc cgctcctggg ccgagtccga gacggcgctc
     3961 gaggcgctgc gggtcgccga ccgggtccgc cggcaggtcg agcaccgtcc cgtccggagc
     4021 gaaccgaccg gccccagcgt tccggtctga ccttcccgcg cctggtcatc gcggccggcg
     4081 acccctcggg ggaccgcctg ggcgccgcac tcctcgccgc gctccgcgcg cgtggcgtgc
     4141 gcgtggaggc ggccggcctg gccggccccg cgctccgcga agcgggagtg ctcgcggccg
     4201 cgccgatgga atcggtcgcg gtcatgggat tcgccgaagt gatccgccat ctcccggcca
     4261 tccgccgtgc gcgcgccgcc gtgctcgcgc tcctcgagca ggaccccgaa gcgatcttcc
     4321 tgccgatcga ctcgcccggg ctgaatctcg ggctggcccg cgccgctcat cgcatggggc
     4381 gccgcgtggt ctactacgtc ctgccccagg tctgggcctg gggctacggc cgcgtgcgcc
     4441 ggatccgcga ggacgtggat ctcatgctgc ttctcttccg tttcgaggag gcgatcgcgg
     4501 cgcgggaggg gatcgccgcg cgctgggtcg ggcatcccgc gggggcgctc gctccgccac
     4561 ccgccgcacg ccgggacgcg cggtccgcgc tggggatcga ggacgacgcg accttgatcg
     4621 cgctccttcc gggaagccgc gtcggggaag tccgccgcca tctcgcgccg atgctcgatg
     4681 ccgcggcacg gctggccggc gcgtgggaga agccggtccg tgtggcggtg agcgacgccg
     4741 gcccggtcgc ggggaccgcg gcgcgcggcg acgcggcgcc cctctggaac gcggtgcgcc
     4801 ccctgcgcgt cgccggcacg tcggcgccgc tcctcgccgc ggccgacctc gccctggtcg
     4861 cctcgggcac cgcgacgctc gaggccgcgg taatcggcgc gcctttcgtg atcgtctacc
     4921 gcaccgggcg gctcaactac gagatcgcgc gccgactggt ccggctgccg cgcgtcgggc
     4981 tcgccaacat cgtcgtcgga gccgacgccg ctccggagtg catccaggac cgcgccaccg
     5041 ggatcgagat cgcgcgcgcc gcggcgccgc tgctcgtgga gtcggcgcgc cgcgaggcgc
     5101 agcgcgccgc gttcgctccc ttgccggagc tcttgggcgg cgggggctcg gccgaccgcg
     5161 cggcggaggc gctgatcgag ttcgtcgagc gcggcaaggt cccggcggcg gcgggcgcgg
     5221 cgcgtgagg
//
LOCUS       DASSLZ010000022         6228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_261988, whole genome shotgun sequence.
ACCESSION   DASSLZ010000022 DASSLZ010000000
VERSION     DASSLZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261988"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VFM00_00755"
     CDS             complement(<1..890)
                     /locus_tag="VFM00_00755"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7496808.1"
                     /translation="MARAAATPAPAAASAASKVSLPPARTVTLPNGARLILAERHDVP
                     LISFGGYIRGAGDLTDPPGKEGLASLTADLLRKGAGKRSAREIAAVVDGAGADLSTDA
                     GVETATISGKFMARDQALMIELIRSLLREPAFPDSEFTKLKEQTIEGLKAQKDDLYSM
                     LGLYAAAYFYGSHPYGRPVTGDEATVATITRDDVLACYRQNYGGDRLILAMVGDFDAR
                     AMEAKLRSALGDWHAAASPAPAVPPTSRRTGRRILLVNRPDATQSYFWIGNLGVSRTD
                     PDRVAIDVVNTAFGGRFTSML"
     gene            complement(980..2407)
                     /locus_tag="VFM00_00760"
     CDS             complement(980..2407)
                     /locus_tag="VFM00_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7496809.1"
                     /translation="MRACCSSCLAAAVGVTLLLAAGALPALGQPRERDSARPGSAPRG
                     VPPEIATKVLPNGLQIIVWPDHDISNVALYNFVHVGSRNERPGITGLSHFFEHMMFNG
                     SAHHPPGDFDRVMEENGGSNNAYTTNDVTVYQDWFPRSAMELIFQLEADRICCLSFDS
                     TVVESERQVVYSERRTSVEDDNSSLLDEQVMATAYVAHPYQIPVIGWPSDIEHWTLND
                     LKSYFHTYYAPNNETMVIAGDVTPAEVFALAERYLAPIPAQPQPARVGPAEPAQLGER
                     RVEVRKSGQTPLIEVAYHVGTPRDPGAEALDLLHMILTSGESSRLYRRLVDQERIAVG
                     VGSSVMAGFDPGLITFQVTVSPGRSTADVERILYEELDRLAREGPTEGELTKAKNIQL
                     AAYWETLETINGKASALGDNQVFHGDWRKLFSSPSRYAAVKARDVQALAKRIFVETNR
                     TVGVLVPEETRRAGDGAPARAGGAR"
     gene            2537..3337
                     /locus_tag="VFM00_00765"
     CDS             2537..3337
                     /locus_tag="VFM00_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HET7496810.1"
                     /translation="MPTMHEVDYEIKGDDMQFVRIQLDPGEAVVAEAGAMMYLEDGIQ
                     MDTVFGDGSQQNQGFMGALMGAGKRLLTGESLFMTVFHNESREKRWCAFAAPYPGKIQ
                     AMNLAQLGGEIIAQKDSFLAAAKGVSIGIAFQRRFGVGLFGGEGFIMERLQGDGWVFI
                     NAGGTMEEHDLKAGETLRVDTGCLVAVQPTVQYDIQFVGKLKSALFGGEGLFFATLQG
                     PGRVWLQSLPFSRLASRIYAAAPQTGGRRKEEGSILGGLGNLLDGDGR"
     gene            3686..4240
                     /locus_tag="VFM00_00770"
     CDS             3686..4240
                     /locus_tag="VFM00_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7496811.1"
                     /translation="MTAPTTLFQTLPTAGEGVDVARAQSGDTRAFERLYRTHVSRILG
                     LVRRMMGSDHAHEVTQDVFVRAWEKIGTFRGEAQFGTWLHRVAVSVILHRRNALRIER
                     GRIKDDEDALEAAPARPHTADLGMDFERAVEKLPAGARMVFVLHDVEGFKHEEIAELM
                     GVTSGTTKAQLHRARMILRQHLDR"
     gene            4266..4946
                     /locus_tag="VFM00_00775"
     CDS             4266..4946
                     /locus_tag="VFM00_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7496812.1"
                     /translation="MSDRYTDRLSDYLDQELEGAERADLERHLLECEECVSTLAGLKR
                     VVARAGGLADETTAPAPDLWPGIEARIRQPRPRAVPIGRAGASDRRRSFSVPQLLAAC
                     LVAAVLSGTAVWFAQRGAPHETGELIPGSTSRNAAAETASSPAANATAVEELRAALAS
                     GRDDLDPATVRTLEESLMVIEIAIREARRALDADPHNPYVRAHLDETMRRKVELLHRA
                     TMLASATR"
     gene            4959..5951
                     /locus_tag="VFM00_00780"
     CDS             4959..5951
                     /locus_tag="VFM00_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET7496813.1"
                     /translation="MLPALAVAAGLASFSLAPGADTVLAVPPGFSLNVANYAGEVFVE
                     GWNRNAIRIRSEHSDEDRVTVLSGRGIVMVKAASKHEEDREANLRLQVPVWMNVSISG
                     IHTDVIVNGTQGQVKVETVHGDVIVRGGRRHIDLNTVNDDICVSDAAGTIRAETVNGD
                     AYVWRAQSDSVEVSTVNGDVVYEGSMGDAGVYRFASHNGDVAVTVPRAANAAIAVSTF
                     AGDFESNFPVTLSGTHNPKRFQFCVGNCSARVDLESFQGTIQLYRAAEAASARVQRMD
                     QIVERIVEQRERTDDLQSKIEQARRGVKKVFERSKKRHEIHETHDEELGGGGGE"
     gene            complement(5953..>6228)
                     /locus_tag="VFM00_00785"
     CDS             complement(5953..>6228)
                     /locus_tag="VFM00_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7496814.1"
                     /translation="MTKNAAIRYAKERIRVNSLHPGFIDTPMIAPFMKDAAGQKSGMA
                     QWIETMTPMGRVGTPEEVAEVIVFLVSPGASYMTGSEVYVDGGWTAA"
BASE COUNT          990 a         2092 c         2170 g          976 t
ORIGIN      
        1 agcatggacg tgaagcgccc gccgaaggcg gtgttcacga cgtcgatcgc gacgcggtcg
       61 ggatcggtgc gcgaaacccc gaggtttccg atccagaagt agctctgcgt ggcgtccgga
      121 cggttcacga ggaggattcg ccttccggtg cgtcgcgacg tcggcggcac ggccggcgcg
      181 ggagaggcgg cggcgtgcca gtcgccgagc gcgctccgca gcttcgcctc catggcgcgc
      241 gcgtcaaaat cgcccaccat cgccaggatc aggcgatcgc cgccgtagtt ctgacggtag
      301 caggcgagca cgtcgtcgcg cgtgatcgtc gcgaccgtcg cttcgtcccc cgtgaccggg
      361 cgcccgtagg gatgagagcc gtagaagtag gccgcggcgt agagcccgag catggagtag
      421 agatcgtcct tctgcgcctt caacccttcg atcgtctgtt ccttcagctt ggtgaactcg
      481 gaatcgggaa aggccggctc gcggaggagc gagcggatca gctcgatcat cagcgcctgg
      541 tcgcgcgcca tgaacttccc ggagatcgtc gccgtctcca cgcccgcgtc ggtcgagaga
      601 tcggcgccgg cgccgtccac gaccgccgcg atctcccggg ccgaccgctt ccccgccccc
      661 ttccgcagca ggtccgcggt gagcgatgcc agcccctcct taccgggggg gtcggtgagg
      721 tcgcccgcgc cgcggatata gccgccgaag gagatcagtg ggacgtcgtg ccgctcggcc
      781 aggatgagcc gggcgccgtt gggaagcgtg accgtgcgag cgggtggcag cgagaccttc
      841 gacgcggcgg atgccgccgc cggggccggc gtggcagccg cccgcgccat gctggccggc
      901 aaagcgcacg caagcgccat cccaacgagg gccgctgagg aaccgaggcc gtgcgacacg
      961 aagcaccgcg cccacggcgt catcgcgcgc ctcccgcgcg cgccggcgcg ccatcgcccg
     1021 cgcggcgcgt ttcctcgggc accagcacgc cgaccgtgcg attcgtctcg acgaagatcc
     1081 gcttcgccag cgcctgcacg tcgcgcgcct tcacggcggc gtagcgcgag gggctggaga
     1141 agagcttcct ccagtcgccg tggaagacct ggttgtcccc cagggcgctc gctttcccgt
     1201 tgatcgtctc gagcgtctcc cagtacgcgg cgagctggat gttcttcgcc ttggtcagct
     1261 ctccctcggt gggaccctcg cgcgccagcc ggtccagctc ttcgtagagg atccgctcca
     1321 cgtcggccgt gctccgccct ggagacaccg tgacctggaa ggtgatgagc ccggggtcga
     1381 atcccgccat cacgctcgag ccgacgccga ccgcgatgcg ctcctggtcc acgaggcggc
     1441 ggtagaggcg cgacgattcc ccggaggtga ggatcatgtg gaggagatcg agcgcctcgg
     1501 cgcccgggtc gcgcggcgtg ccgacgtggt aggccacttc gatcagaggg gtctgcccgc
     1561 tctttcgcac ctcgacgcgc cgttcgccca gctgcgccgg ctccgcgggc ccgacgcgcg
     1621 cgggctgcgg ttgagccggg atcggggcca gatagcgctc ggccagcgcg aagacctcgg
     1681 ccggcgtcac gtctcccgcg atcaccatcg tctcgttgtt cggcgcgtag tacgtgtgga
     1741 agtagctctt caggtcgttc agcgtccagt gctcgatgtc gctcggccag ccgatcacgg
     1801 ggatctggta gggatgggcg acgtacgccg tcgccatcac ctgctcgtcg aggagcgacg
     1861 agttgtcgtc ctccaccgac gtacggcgct cggagtagac cacctgccgc tcgctctcca
     1921 ccacggtcga gtcgaacgag agacagcaga tccggtcggc ctcgagctgg aagatcagct
     1981 ccatcgccga gcgcggaaac cagtcctggt agaccgtgac gtcgttcgtg gtgtaggcgt
     2041 tgttcgatcc gccgttctcc tccatgacgc gatcgaagtc tccgggtgga tggtgggcgg
     2101 agccgttgaa catcatgtgc tcgaagaagt gggagagtcc cgtgatcccg gggcgttcgt
     2161 tccggcttcc gacgtgcacg aagttgtaga gcgccacgtt cgagatgtcg tgatccggcc
     2221 agacgatgat ctggaggccg ttggggagga ccttggtcgc gatctcgggc ggcacgcccc
     2281 gaggagccga acccggccgg gcggagtcgc gttcgcgcgg ctggcccagg gccggcagcg
     2341 ccccggcggc gaggaggagc gtcacgccca ccgcggccgc gaggcagctg ctgcaacagg
     2401 ctctcatcac gtcacctccc gggtcttcga aaccgcgcgg gcgcgcggga cgtaccgttt
     2461 tcgagtctag caacctggcg tatccttgtg aggcggattc gccgaagagg gtttgaaatc
     2521 atccaggggg gcgcccatgc cgaccatgca cgaagtggat tacgagatca aaggggacga
     2581 catgcagttc gtccgcatcc agctcgatcc cggcgaggcg gtcgtcgccg aggccggcgc
     2641 catgatgtac ctggaggacg gaatccagat ggacaccgtc ttcggggacg gctcgcagca
     2701 gaaccaggga ttcatgggcg cgttgatggg cgccggcaag cgcctcctca ccggcgagtc
     2761 gctgttcatg acggtcttcc acaacgagtc gcgcgagaag cgctggtgcg cgttcgccgc
     2821 gccctacccc gggaagatcc aggccatgaa cctggcccag ctgggcggcg agatcatcgc
     2881 ccagaaggac tccttcctcg cggccgcgaa gggcgtgagc atcggcatcg cgttccagcg
     2941 ccgcttcggc gtcgggctct tcggcggcga gggcttcatc atggagcggc tgcagggcga
     3001 cggctgggtc ttcatcaacg cgggcggcac catggaagag cacgacctga aggccggcga
     3061 gaccctgcgc gtggacaccg gctgcctcgt cgccgtgcag cccacggtgc agtacgacat
     3121 ccagttcgtg ggcaagctga agagcgcgct cttcggcgga gagggactct tcttcgccac
     3181 gctgcagggg cccgggaggg tgtggctcca gtcgcttccc ttcagccggc tggccagccg
     3241 gatctacgcg gccgcgccgc agacgggggg ccggcggaag gaggagggct cgatcctggg
     3301 cgggctgggg aacctgctgg atggagacgg aaggtaggct gcccgggaac tggagcacga
     3361 aagaccatgc cccggggcgc tccggcgctc cggggcttca tgtttccgga gcccctctcc
     3421 ttacccgggc ttcattttct cccgacgagc cacaggatca gcgtgagcag gggctcagtg
     3481 tcgcccatgc tcgacttgcg ccggcgcaag ttaagtttta tgttaatttt cagggctcgc
     3541 ctgccaaaac ggcaggcggg caagtgattc cgctaggggg gccccggagc gcacccctac
     3601 actttcccca atgcctcccg acattccaac caatgccccc tcgtttccgt ccaagtcatt
     3661 gcacgaagcg ctcgtggaga ccggggtgac ggcgccgaca acgttgttcc agacattgcc
     3721 gacggcagga gagggggtcg atgtcgctcg agctcagagc ggcgataccc gcgcgttcga
     3781 gaggctctat cgcacgcacg tctctcgcat cctgggactg gtgcggcgca tgatgggcag
     3841 cgaccacgcc cacgaggtga cgcaggacgt cttcgtgcgg gcgtgggaga agatcggcac
     3901 cttccgcggc gaggcacagt tcgggacctg gctccatcgc gtggcggtga gcgtgatcct
     3961 gcaccgccgg aacgcgctcc gcatcgagcg cggccgcatc aaggacgacg aggacgcgct
     4021 cgaggcggcc cccgcgcgtc cccacaccgc ggacctcggg atggacttcg agcgcgccgt
     4081 ggagaagctc ccggcgggag cgcggatggt cttcgtcctg cacgatgtcg aaggattcaa
     4141 gcacgaggag atcgccgagc tgatgggcgt gacctcggga accaccaagg cgcagctcca
     4201 ccgcgcccga atgatcttgc gtcagcatct ggaccgctga gagacgagag gaacggagca
     4261 ccacgatgag cgatcgctac accgaccggc tttccgacta ccttgaccag gagctggagg
     4321 gggccgagcg cgcggacttg gagcggcacc tgctggaatg cgaggaatgc gtctccacgc
     4381 tggcgggctt gaagcgggtt gtcgcccgcg cgggcggcct cgccgacgag accacggcgc
     4441 cggcgcccga cctgtggccc gggatcgagg ctcggatccg ccagccgcgg ccccgcgccg
     4501 tcccgatcgg ccgcgccggc gcgtccgacc ggcgccgctc cttctccgtg ccgcagctct
     4561 tggccgcgtg cctcgtggcc gccgtgctct cgggcaccgc ggtctggttc gcgcagcgcg
     4621 gcgccccgca tgagacgggc gagctgattc cgggttccac ctcgcggaac gccgccgccg
     4681 agacggcttc gagtcccgcc gcgaacgcga ccgcggtcga ggagctgcgc gcggcgctcg
     4741 cgagcgggcg agacgatctc gatcccgcca ccgtgcgcac cctggaagag agcctgatgg
     4801 tcatcgagat cgcgatccgt gaggcgcggc gcgccctgga tgccgatccg cataacccct
     4861 acgtccgcgc gcacctggac gagacgatgc gacgcaaggt ggagttgctc caccgcgcga
     4921 ccatgctcgc cagcgcgacg cgctgaagga atccccccat gctccctgcg ctcgccgtcg
     4981 ccgccggtct cgcctccttc tcgctcgctc cgggcgcgga caccgtgctc gccgttccgc
     5041 ccggcttctc gctgaacgtg gcgaactacg cgggcgaggt cttcgtcgag ggatggaacc
     5101 ggaatgccat ccggatccgt tccgagcact cggacgagga ccgcgtgacg gtattgtccg
     5161 ggcgagggat cgtgatggtg aaggccgcct ccaagcatga agaggatcgc gaggccaacc
     5221 tccggctcca ggtcccggtg tggatgaacg tgtcgatctc cggcatccac accgacgtga
     5281 tcgtgaacgg cacgcagggg caggtgaagg tcgagacggt gcacggagac gtcatcgtgc
     5341 gcggcgggcg gcgccacatc gacctgaaca cggtgaacga cgacatctgc gtgagcgacg
     5401 cggcgggtac gatccgcgcc gagacggtga acggcgacgc gtacgtctgg cgcgcgcagt
     5461 cggacagcgt cgaggtctcc acggtcaacg gcgacgtcgt gtacgagggt tcgatgggcg
     5521 atgccggggt ctaccgcttc gccagccaca acggggacgt cgcggtcaca gtgccgcgcg
     5581 cggcgaacgc cgcgatcgcg gtctccacct tcgccggcga tttcgagtcg aactttcccg
     5641 tcaccttgag cggcacccac aacccgaagc gcttccagtt ctgcgtgggc aactgctccg
     5701 cgcgggtgga tctcgagtcc ttccagggca cgatccagct ctaccgcgcg gccgaggccg
     5761 cctcggcgcg ggtgcagcgg atggaccaga tcgtggagcg catcgtcgag cagcgggagc
     5821 ggaccgacga tctccagtcg aagatcgagc aggcccggcg cggggtgaag aaggtgttcg
     5881 agcgctcgaa gaagcggcac gagatccacg aaacgcacga cgaagagctg ggcggcgggg
     5941 gcggcgaata gatcaggcgg cggtccagcc tccgtccacg tagacttccg agcccgtcat
     6001 gtaggatgcg cccgggctca cgagaaacac gatcacctcg gccacctcct ccggggttcc
     6061 caccctcccc atcggcgtca tcgtctcgat ccactgcgcc atcccgctct tctggccggc
     6121 cgcgtccttc atgaagggcg cgatcatcgg ggtgtcgatg aacccggggt ggagcgagtt
     6181 cacgcggatc ctctccttgg cgtagcggat cgccgcgttc ttggtcat
//
LOCUS       DASSLZ010000023        22120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_8941, whole genome shotgun sequence.
ACCESSION   DASSLZ010000023 DASSLZ010000000
VERSION     DASSLZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 22120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22120
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8941"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VFM00_00790"
     CDS             complement(<1..1065)
                     /locus_tag="VFM00_00790"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET7496815.1"
                     /translation="MLHRGGPFGVRPASFGAVPAVVVALVVAAVCADAGSAGASPVCA
                     RGPLADSVRAAVDLLDGQADVGAIAVSLDRGDTLFALNEDRRLIPGSNTKIFTTAPFL
                     RRYGPSARFPTTIEARGRVEQRKDGVRLQGDIVLRPAGVPDVTQILAPGSRDLLDSLA
                     ILLRAGGLQRFEGTLWVDGTLFADEGYGPGWAVEDVPWSYGTLPNAVMANGNAATLIA
                     TGGPKDVDLTLDPPETPIAIAARPAALPEGSAGAIDLDRALGSRSLRVSGAVPRGVTL
                     KKQISVPEPDSAAGLWLLGAMRRAGIEVKRAEVRLVPRETRGAGLLGGAGSRSGSGKG
                     GGASGKGGAAPERAAAADTGW"
     gene            complement(1083..2129)
                     /locus_tag="VFM00_00795"
     CDS             complement(1083..2129)
                     /locus_tag="VFM00_00795"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET7496816.1"
                     /translation="MKRFLMQWVLPAAMLAQAATAGAGGLDRIGTSGAQELRIPVGAA
                     SIATGGSTVAVGDGLRNMYWNPASLAGTDQSEGMISYSSYLGDSKVNYGAISTHMGSQ
                     GQIAFSAKVLNIGDIIVTTEDAPDGTGEVLNPNFGVFGITYGRRMTDRVHLGVSGAYI
                     SEKVADVSATGFAIDLGVQYDTGWRGLRFGFAMKNIGPDMRFSGSNLEQRVVLPSDDP
                     ASSGHVLQLQAASFEMPSYLQIGVSYDLPMGGSERHATVMGAFQGNNFTTDEYHVGAE
                     VPIGNFLALRGGFVGQVPIKEADRQPNYLYSYSYGAGFNFKLGDRPLALDWAGQHVGD
                     FFDDNQQVSLHIAF"
     gene            complement(2192..5155)
                     /locus_tag="VFM00_00800"
     CDS             complement(2192..5155)
                     /locus_tag="VFM00_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496817.1"
                     /translation="MKSLKWIGAAALLTLLAAPGVLAATRVVYVPSHRATKAQRAAQT
                     IDNTGRMNVNNLDMVVTNHGSLAYDLLTGNAGLIYPRGTIKTAVFAAGLWIGAKVGGE
                     IRAAVGEYSQEYTPGPMAGGTFQSDNPSFHNFRIARGSTLTGTDLTDYIAQGGPLDST
                     GAPQLLGDATIWSVFNDADPGVHTNEAGSTAPLGVEVQQSVFAYNRSGALGNIIFVKW
                     KIINKGANTLDSTYVSVWSDPDLGGATDDLVGCDTTLSLGYCYNATNSDGLYGATPPA
                     VGYDFFKGPSVAGNPLGMTSFNKYVNGTDPSSQVETYSYMSGLNKDGSPIHVLNDTTL
                     AITKFQVSGLNLNAASTATNWLDSNPGDRRLQLSSGPFTMAPGDTQEVVTAIIIGQGT
                     DRISSVSDMKNKDTAAQTVFDLNFDIPQPPPSPTVYVQPLDRAVRLIWDRTAVGTHSA
                     NAFLGQDFVFEGYRVWQLPSAGGGTPKVIATFDVAGNAIGPIYSDLFNSAVGAVERTL
                     VINGTDEGLRFQLDITNDAIRGGRLVNYKDYYFAVTAFSYDSLNVTPYVLGVNQLGIV
                     SDVLESALNVVHAVPKGSNAIFTVPTTQIAGSFVGNTFAVQQISSTVADSTYHLVVTG
                     PNGLFSIVNTVTGDTIVTNQTSAIVNGFTPVFTENTTPAVIQQLEGGTCLACAADSIS
                     FMAGPAVVDSSGTYILSNYLHYPDGPVDPSNWIFDGDLPDLAHDFLIRVLPDTTEYAW
                     RYAGGADSPQATFKVPFELYDLGLCSLQDPSDDVRITPEIRDRNSNGKFDWGDALYVR
                     DIPYASVAWNTAITSGDYSTDDESYGRLTFYQAPGSTATLPAPGRILIRGPALCPGDV
                     FAFRTVPAGAAPGSVVGNDLTKIRAVPNPYYAHSQYELSQFDRVLKFTNIPGSREVTI
                     RIFNLAGDLVRTIRRSAQAGNDMSSSQITWDLNTENRLPVASGIYIARIEVAGIGATT
                     LRVAVFVEQERLDNF"
     gene            complement(5187..8156)
                     /locus_tag="VFM00_00805"
     CDS             complement(5187..8156)
                     /locus_tag="VFM00_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7496818.1"
                     /translation="MVRPRTLLSLGVATFIVVGAFFVAPDARAGTTGQISGTVRDGKT
                     GEPIGLASVTIPDLKRGAVTDATGNYFILNLPPGKYTVRVALLGFVPQAREGVEVIPD
                     FNAKLDWSLESTVLKDVAEVTVKAERPLIQKDVTGTTKFLEGDQIRNQPLRGYKDAVA
                     QQSGVVNFQARLDNPQLNGNESLNGSTLIIRGGRPNEVAYYVDGFSQQDPLTGFSTTA
                     INNDAVSEIVVQSGGFNAEYGRINSGVVNVVTREGSDKYFGSIEGVTDNLAGSWISAP
                     RRDNNTYALSLGGPIVPSLKQVNFYLSGERRFNRDRNPSFETDKVAYAPQGEISTDFF
                     EKGILPANSSASWATVGKLTIKPSPLHTLKIGGTLNDENWQQYLNSYRFDLRHAPRYE
                     DKNRSVYGTWNQSLSDRAYYEVKANYFLTERMRGDGVAFDDLRGYSRPNGNPSYDQSE
                     ALFWYGDASATGPHVYDDFLHRKSSYVGFAANYANQLTRSFQLKAGGDYQRHTLRYYD
                     HYFPTQLYDAAGNPDNTRDVDRYGYDALGNELNEGDTFTDTNGNGSYDVGEPFSDKNG
                     NGLYDSPLDVPKHPKVASLYVQGKYERLGLVLNTGLRWDYLTPSTDALRSETLPLDPD
                     ATGTDSRLQATDLQKSKVYQRISPRLGVGFPVSDQTLLHVNYGKFFQQPNLQDLYVSY
                     AFLEHKIRTGGYFVGFGNPNLKPEQTTAYELGIQHTPNDRSRVEASAYYKDVKDLVEI
                     TNIPSAPNAFSSYRNRDFATIKGLDLSYTLRRTGLVSFNASYSLSWARGTGSLSQTQR
                     NIAWYAAETPKMSSPLAFDQRHKLSANFDFRYDKGQGPTWHGNHILENAGINLLANAS
                     SGTPYTPTKVYNEVTLANTASEPSGPVDARYGPWTFQVDAKMGKTIAIGRQNLELYIW
                     ALNIFNRDNVYTVYTSSGSAVTTNWLETAEGQAFIEGNASKYGTDGAIQRYQLAEQSP
                     LLHGVPRMVRFGAKLNF"
     gene            complement(8533..9855)
                     /locus_tag="VFM00_00810"
     CDS             complement(8533..9855)
                     /locus_tag="VFM00_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496819.1"
                     /translation="MSQRKLIVSLTIAVLLAISAGVSQASLSRMEGMGLDVPLLSQFT
                     DDYANIYTYPTSVVRQNNLVLAELGQNPNDGEANPVDVTDQTYTVIRNFPRIGAIAFQ
                     MAQSQTDVLTGSGNLNNEQLDMIWGRAFSNFDFAVRLDITNSKFEESTNAPASIELHG
                     NSPFDPGFGVIPFFVDVAPDAILGNGIELNTYGITPSFALHFGEDDRFEAAATYRRYT
                     LDRTQTPAGGESWQDQGNASYGAFARLIMHRGGTHTWYPAAWYVNDDLSWEMTGLGAR
                     PTVSADETYKNYGLGISDNMRVNDNNLLLWGVSVGQFKHEYERTDQNTGGALAELHTF
                     EDKSTMLPLVFAAIETEATSWLKIRIGASRGHLVERTESADFNTPSNTDLTKESLADF
                     NLGLGAGIRWNNLDIDMAINNQFPLSGGWILSGNEATPFNRVSATYHF"
     gene            complement(10233..11462)
                     /locus_tag="VFM00_00815"
     CDS             complement(10233..11462)
                     /locus_tag="VFM00_00815"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HET7496820.1"
                     /translation="MPGTSGDRPRFSVDGTVEPGDGGVPLVRLDYRLARAELLFERRP
                     GGYHASYEVSVVFYKEKGGRQVTGDTFVRDIDVKTYAETRPIGNDIIDHVDLPAPPDK
                     YEVQVVIRDLVAERASGTRIDLEVQKQAAGPIGFTDLSFGNVKGGVPPGSDPRAALVP
                     NPARRYSEDVTTLAVYGEVVDSRPAAGFDPGYQIQYRILNDQQERVLERDTTLARAGA
                     RTPFLLRPSAEALVPGNYRFLVELKAPLLPQPGGKRQPIRREKSFEVVQSAATIVADP
                     KTTLEILAYIADSDEKKEMDAAKTTEQKREFWEAFWKRRDPTPDTPENESMVEFYRRI
                     AYADQHFGVAGPGWKTDMGKVYIKYGQPDEVVRSPFAIDKHPEEIWYYYQQRKTFYFV
                     DKYEFGRYDLDPRSSAL"
     gene            complement(11596..14181)
                     /locus_tag="VFM00_00820"
     CDS             complement(11596..14181)
                     /locus_tag="VFM00_00820"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7496821.1"
                     /translation="MVASYQMNSRRLRRRRSWAQLAAVGSLLVAAPPAAHAIPETVAL
                     RGVPSSLAASFGPAVTVGAGVIAVPTGSGADLYDLSQPSTPLLGRFRTAGNVARASVA
                     GSTLFLFAGPRGVVAVDVSNPANPVAAGSAGDLGTVTLGAAASSGGGVVAGGGSTLHF
                     LSWDAASGFTLRRTLVYSDGRTVGAVAARGDSFLVASVRGTLPTRLILTLYRMRAGAA
                     APDSLEEFAFNGHRADDLAWVGAVAFVADGNLGVLAIDVAAHALRSVTPVQGTQFVRS
                     VDANDSVVVAATEGKTLARFARVGAAGDTLTGEIDRVLTGNPAYARLSGDWIVASTYD
                     QIAPAPPDEPGRSLLELVSLSGAPDRAPVGATGRVRRVAVKNGLAYVANYSGGLRIYR
                     AGGGDSSLVGVAPPSGNARPVDVAVDPALPLVYLASGPAGLEVVQVGDPAAPIPVATL
                     TLAGSASAVTQVGPNLVAVATRGLNPAVTFVRVDYQPLDSTVTLNARGSVGNPFVQDP
                     RGLAARDTVLFVADESLGLLSIGFGNPDQPAAFGTTSLAGARDVDLSGTLLLVATRTR
                     GLQIVDVANPAQPVLRAELATPPLLGVARNGTSAVVFAGDLGALVVDVGVPSAPVTRG
                     PILPPGVARDGWWTGDTLLVAASLGLERYLVSPAPTVVPVLDVRLDPASATPRALVTW
                     TPVVRAGMVGLNLYRDLVTASGTASDPAGRRVNRDLLPPGAVSAVDDSLIAGGALRYR
                     LEAFFADGSSVKVAEGTLSVASAPLVGRAYPNPFRARPGAVASLSYRVPNGSAGAPLT
                     LRVFDVSGRVVRESRAPAQPVSGFGIMTWDGRDRNGGAAPSGVYYLRLTGAGLDDSRA
                     IILLR"
     gene            14255..14893
                     /locus_tag="VFM00_00825"
     CDS             14255..14893
                     /locus_tag="VFM00_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET7496822.1"
                     /translation="MPSLDTILAPLSTFILSTISSWGYAGIVACMTIESACIPLPSEI
                     IMPFSGYLASTGRFTLWGVALAGGVGNVFGSAIAYAAGRYGGRPLAERLARWRVLRMS
                     DYDRGNLWLQRHGVAVAFWSRLLPIVRTFISFPAGAARVPFGIFSLYTFIGSVIWAMA
                     LAWVGVLLGEHWHDIRKVMRGFDLVVVAAILMAGVIWARHHFRADPGGPAPE"
     gene            15120..15193
                     /locus_tag="VFM00_00830"
     tRNA            15120..15193
                     /locus_tag="VFM00_00830"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15152..15154,aa:Gln,seq:ctg)
     gene            15232..15306
                     /locus_tag="VFM00_00835"
     tRNA            15232..15306
                     /locus_tag="VFM00_00835"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15265..15267,aa:Glu,seq:ctc)
     gene            15373..18114
                     /locus_tag="VFM00_00840"
     CDS             15373..18114
                     /locus_tag="VFM00_00840"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7496823.1"
                     /translation="MLLLFGLLAAAAPVAGAQPRPTHSRASRALGNVERIDSLGGITE
                     YRLRSNGMKILLSPNHAAPVITFMVVYHVGSRNEAPGNTGSAHLLEHMLFNKSTKNFG
                     KANHKKTVQEVLYESGADYASSNMTTWYDRMNGYSTLPAEKLETAMKIEADRLGRALI
                     LDSERQPEMSVVRNEYEIGENSPYRALDKAVIAEAIQAHPYHWNTIGYRSDIEGVTTE
                     KLREHYRDFFYPDNAEAILVGDFDTAAALAMFDREFAAFARSKTPIPQVITVEPPQEG
                     ERRVTVRRPGQVGIVEIAYMRPNALDPDYVPLDVLSSILTAGVNSRLYQALVEPGLAT
                     DVDSWNYTLRDPYPIVAEATVAPGRSHDDVERAMKAALYRIGKEGVSATELDRAKSQL
                     EVSVVRGRDGTYELASSLGEAVASANWKWFVGYVDAVRSVSADDVKRVAAKYLVPDHA
                     TVGWFVPASGEKTEAKVGLAPAPVRDKRAGAGGASEAAASGFGSRTLHKVLGNGLTLD
                     VVVNRAVPTVAIQGTVFAGRMEAPGGKPAVPDLTAIMLTRGTKRADKRVIAARLDGVG
                     AQLQIFAGVTEATIVGNSLSRDLRLLLGTLADELANPAFADSEVAKAKLEMRANVLRS
                     SESTAARGLDRLTRLAYPEGHPYRAATTDAMLASLDKANAADLRAFWRARYGGAGIIL
                     AVVGDVDPQAVAALADSLFGGIERGARPTHDAAPTAPNAPEHVVETIRGKANVDFLYG
                     QASGLKRNDPDYEAALVANAALGQSSLTSRIGKRVRDTEGLSYNLYSRWFWPDYADGL
                     WAVDVAVAPQNVAKALRSTKEEIDRYGKEGITDAEVEVQREFFAGNYLVRLGTNAGVA
                     AALCTAEKFGYGPGYLDDFPKRIRAVTKAQVDDVLRRRFHPDKMHLVVAGDLDSIPQ"
     gene            complement(18140..20989)
                     /locus_tag="VFM00_00845"
     CDS             complement(18140..20989)
                     /locus_tag="VFM00_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7496824.1"
                     /translation="MSLPTGGFQRFRFLTALMAGACAAVLVLLAWPVSPLHSSTPALS
                     DFTNFESLPVHPLALTPDGTRLLACNLPDARLEIFTIGDAGLTSAGEVPVGLEPVSVA
                     ALNDSIAWVVDNVSDDVSIVNLNSKRVAATLRVGDEPTDVVLAGSPRRAFVCVSGEDA
                     IKVYSLAGEAGTTLDLVRPVFGRHPRALAARGGEVYVAVLDAGNRTTTLSATEVQSGG
                     GPPPPNPPLKAGLPAPPAVGLIVQKVGNDWVDERPASVKTWNTFIPYDLPDQDVVVLD
                     AASGAVLRTVSGVGTNLFDVAAASGTGDLYVSNTEAQNRTRFEPNLRGRFLRNRLTRI
                     DAGGLGAVTAWHLNAHVVYDTIPGPAGEKALSLAEPTGVAVNPAESKVYVAALGSDLV
                     GVVDPGTGLVTARIPVSAAPRVTKSGPVALALDAARQRLYVLNRFSNSVAVIGTAGES
                     FQSETPLGMGFDPSPPAVTAGRRFLYDGMLSDHGDLACASCHIGGNFDNLAWDLGNPQ
                     GDLQAAPSNPVPIPPFHPMKGPMTTQSLRGLANTPPFHWRGDRADFTRFNPAFVSLMG
                     NIDSLTAAEMQQFNDFILTLAYPPNPNQNLDRTWPNPAAPTPSPTRGKVEFDTKPHDG
                     GTCSVCHSFPTGTNGTLIPAQALQESQAMKVPQLRNLYQKTGFTDAAGPQKRGFGFLH
                     DGSMDNLFDFLNLPVFTFANNQERRDLEAFLLAFDTGTAPSVGREITVNGANRTLTGT
                     TQFLDSLYAAADAGQCDLVAHGRLGGAAKGFYYQAGTRSFLSDYDPEGTFPADSLRLL
                     AQDGGEITYLGVPPGAGRRMGIDRDRDGYRDRWELSLGSNPANPASIPSVSAVAPAPA
                     PPPTRLLANRPNPFNPSTVIPFELGTSGRVALRLYNVSGALVRTLVDAPATPGRYEAR
                     WDGRDNRGLPVASGRYYARLTAAGETKTRTLTLLK"
     gene            complement(21054..>22120)
                     /locus_tag="VFM00_00850"
     CDS             complement(21054..>22120)
                     /locus_tag="VFM00_00850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7496825.1"
                     /translation="HRRGSVAPVIERRKVVESWIARLPFGRSRYRWFVPLYPRAVESF
                     DLSNYDLVISLSHCAAKGVIPRPGALHVCYCFTPVRYFWDLYGEYFGPDRAGPLTRAA
                     AALVAPGFREWDRASSERVHLFVADSKHVGERIARHYGRPAAVVYPPVDTAFFTPASG
                     LTQDPGAPFLIVSALVPYKGVERTIRVVNRMRVPLRIVGTGPEEARLRRLAGPTVRFD
                     GWLSAEALREAYRGCRALVQAHEEDFGIAPLEAMACSRPAVALARGGASEVVAEGTGI
                     LYEGDDDAALEAAFESLSRRSFDPAWLRRHAQTFDRARYRERMDALLREAWSAFEEGN
                     GDARAAERKLTGCAPELSML"
BASE COUNT         3503 a         7447 c         7611 g         3559 t
ORIGIN      
        1 ccatccggta tcggccgccg ccgcgcgttc cggagcggcg ccgcccttgc cggacgcgcc
       61 accgcccttg ccggacccgc tccgagagcc cgcccccccg agaagacccg cgccgcgcgt
      121 ctcgcgcgga acgagccgca cctccgcgcg cttcacctcg atccccgcgc gccgcatggc
      181 gccgagcagc cagagcccgg ccgccgagtc aggttcgggc accgagatct gcttcttcag
      241 cgtaacgccg cgcggcacgg cgcccgacac gcgcagcgag cgggagccca gcgctcgatc
      301 gagatcgatg gcgccggcgg agccctcggg aagcgccgcc ggacgcgccg cgatcgcgat
      361 cggcgtctcc ggagggtcga gggtcaggtc cacgtccttg gggccccccg tcgcgatcag
      421 ggtggcggca ttcccgttcg ccatgacggc gttcggaagg gtgccgtacg accagggaac
      481 gtcctcgacc gcccaccccg ggccgtaccc ctcgtcggcg aagagggtgc cgtccaccca
      541 gagcgttccc tcgaaacgct ggagcccgcc cgcgcggagg aggatcgcga gcgagtcgag
      601 gaggtccctc gagccgggcg cgaggatctg cgtcacgtcc gggaccccgg ccggacggag
      661 cacgatgtct ccctggagac gcaccccgtc cttgcgctgc tccaccctcc cgcgcgcctc
      721 gatggtggtc gggaagcgcg cggaggggcc gtagcggcgc aggaaaggcg ccgtggtgaa
      781 gatcttcgtg ttgctgccgg ggatgaggcg gcggtcctcg ttcagcgcga acagcgtgtc
      841 tccgcgatcg agcgacacgg cgatcgcccc gacgtcggcc tggccgtcga gcaggtccac
      901 cgccgcccgg accgagtcgg ccaggggccc gcgggcacac accggcgagg cgccggcgct
      961 cccagcgtcg gcgcacacgg cggcgacgac cagcgccacg accacggcgg gcaccgcccc
     1021 aaaggaagcg gggcggaccc cgaagggccc gccccggtgc agcacgctcg cgctaccgcg
     1081 ggtcaaaacg cgatgtggag cgaaacctgc tgattgtcgt cgaagaaatc tcccacgtgc
     1141 tggcccgccc agtcgagggc cagcggacgg tcgcccagct tgaagttgaa gccggcgcca
     1201 taggagtagc tgtagaggta attcggctgc cggtccgctt ccttgatcgg cacctgcccg
     1261 acgaaccccc cgcggagcgc gaggaagttc ccgatcggaa cctcggcgcc cacgtggtac
     1321 tcgtccgtcg tgaagttgtt cccctggaag gcgcccatga ccgtcgcgtg ccgctccgag
     1381 ccgcccatcg ggagatcgta ggagactccg atctggaggt aggagggcat ctcgaacgaa
     1441 gccgcctgga gctgaagcac gtgcccgctg gacgccggat cgtcgctggg cagcaccacg
     1501 cgctgctcca ggttggatcc gctgaaccgc atgtcgggtc cgatgttctt catcgcgaac
     1561 ccgaaccgga gcccgcgcca gccggtgtcg tactgcacgc cgagatcgat cgcgaagccg
     1621 gtggcgctca cgtcggccac cttctcggag atgtacgccc cgctgacgcc cagatggacc
     1681 cggtccgtca tgcggcggcc gtacgtgatg ccgaacactc cgaagttcgg gttcagcacc
     1741 tcccctgtcc cgtccggcgc atcctccgtc gtcacgatga tgtcgccgat gttcaggacc
     1801 ttcgccgaga acgcgatctg cccctggctg cccatatggg tggagatcgc gccgtagttc
     1861 accttggaat cgccgaggta gctgctgtag gagatcatcc cctcgctctg atcggtgccc
     1921 gcgagggatg ccggattcca gtacatgttg cggaggccgt ccccaaccgc caccgtcgag
     1981 ccgccggtcg cgatcgacgc ggccccgacc gggatgcgca gctcctgcgc ccccgaggtg
     2041 ccgatacggt ccagcccgcc ggcgccggcc gtggccgcct gcgccagcat ggcggcaggc
     2101 aggacccatt gcatcaagaa ccgcttcatg gttccgaccc tccgtccctt ggaaggatga
     2161 ttggttggtc tcatgtccgc ccccgttcgc atcagaagtt gtcgagccgt tcctgctcga
     2221 cgaagacggc gacacgcaag gtcgtagccc cgattccggc aacctcgatg cgagcgatgt
     2281 agatgccgct cgccaccggc agacggttct cggtattcag gtcccacgtg atctgggagc
     2341 tggacatgtc attcccggcc tgcgccgacc ggcggatcgt acggacgagg tctccggcca
     2401 ggttgaagat ccggatcgtg acctcccggg agccgggaat gttcgtgaac ttgagcaccc
     2461 ggtcgaactg ggagagctcg tattgcgagt gcgcgtagta cggattcggt accgcgcgga
     2521 tcttggtcag atcgttgccc acgaccgagc ccggcgccgc gcccgcgggc accgtgcgga
     2581 acgcgaagac gtcgcccgga cagagcgcgg gaccgcgaat caggatccgg cccggggccg
     2641 ggagcgtcgc ggtggacccc ggcgcctggt agaaagtgag ccgcccgtac gattcgtcgt
     2701 ccgtcgagta gtcacccgag gtgatcgccg tgttccaggc cacgctcgcg tagggaatgt
     2761 cgcggacata gagcgcgtcg ccccagtcga acttcccgtt cgagtttcgg tcgcggatct
     2821 ccggcgtgat ccggacgtcg tcgctcggat cttgaaggga gcacaggccc aggtcataga
     2881 gctcgaacgg aaccttgaac gtggcttgag gggaatccgc cccacccgca tacctccagg
     2941 cgtattcggt cgtatccgga agcacccgga tcaagaagtc gtgcgccagg tcggggagat
     3001 ccccgtcgaa gatccagttc gacgggtcca cgggaccgtc cggatagtgc aggtagttgc
     3061 tcaggatgta cgtccccgac gaatccacga ccgccggccc cgccatgaag ctgatggagt
     3121 cggccgcgca ggcgagacac gtcccgccct ccagctgctg gatcaccgcc ggggtggtgt
     3181 tctcggtgaa cactggcgtg aatccattca cgatcgcgga ggtctgattc gtgacgatgg
     3241 tgtcgccggt gaccgtgttg acgatgctga acagcccgtt cggccccgtc accaccaggt
     3301 ggtaggtcga atccgccacc gtggacgaga tctgctgcac ggcgaacgta ttccccacga
     3361 aggatcccgc gatctgggtc gtcggcaccg tgaagatcgc gttgctcccc ttcgggaccg
     3421 catggaccac gttgagcgcg ctctccagca cgtcgctcac gatccccagc tggttcacgc
     3481 cgagcacata gggcgtcacg ttcaggctgt cgtacgagaa cgcggtgacg gcgaagtagt
     3541 aatccttgta gttcaccagc cgtccgcctc ggatcgcgtc gttcgtgatg tccagctgga
     3601 agcgaagccc ctcgtcggtg ccgttgatca cgagcgtccg ctcgacggcc ccgaccgcgg
     3661 agttgaagag atcggagtag atgggtccga tcgcgttccc cgccacatcg aaggtggcga
     3721 tcaccttggg ggtaccgccg cccgccgatg gaagctgcca gacccggtag ccctcgaaca
     3781 caaagtcctg ccccaggaac gcattcgccg aatgggtgcc cacggcggtc cgatcccaga
     3841 tcagccggac cgcccggtcg agcggctgca cgtacaccgt cgggctcgga ggcggctgcg
     3901 ggatgtcgaa gttcaggtcg aatacggtct gcgccgccgt gtccttgttc ttcatgtcgc
     3961 tgacggacga gatccgatcc gtgccctggc cgatgatgat cgccgtcacg acctcctgcg
     4021 tgtcccccgg ggccatggtg aatggccccg acgagagctg gagccggcgg tcgcccggat
     4081 tggaatcgag ccagttcgtc gccgtgctcg cggcgttcag gttgagcccc gagacctgga
     4141 acttcgtgat cgccagggtc gtgtcgttga gcacatggat cgggctgccg tccttgttga
     4201 gaccgctcat gtagctgtag gtctcgacct gggacgaggg gtcggtcccg ttcacgtact
     4261 tgttgaacga ggtcattccg agcggattcc cggccaccga cggccccttg aagaagtcgt
     4321 agcccaccgc cggcggcgtg gcgccgtaga gaccgtcgct gttcgtggcg ttgtagcagt
     4381 agcccaggct cagcgtggtg tcgcatccga cgagatcgtc ggtggcgccg cccaggtcgg
     4441 gatcgctcca gaccgagacg tacgtcgagt cgagcgtgtt ggcgcccttg ttgatgatct
     4501 tccacttcac gaagatgatg ttgcccagcg ccccggaccg gttataggcg aagaccgact
     4561 gctgcacttc gacgcccagg ggcgccgtgc tccccgcctc gttcgtgtgc acgcccggat
     4621 cggcgtcgtt gaagacgctc cagatcgtcg cgtcgcccag tagctgcggg gcgccggtgc
     4681 tgtcgagcgg gccgccctgc gcgatgtagt ccgtgaggtc cgtgcccgtc agcgtgctcc
     4741 cccgcgcgat ccggaaatta tggaaggacg ggttgtcgga ctggaacgtg ccgccggcca
     4801 tgggaccggg cgtgtactcc tgggagtact ccccgaccgc cgcgcggatc tccccgccca
     4861 ccttggcgcc gatccagagc cccgccgcga agacggcggt cttgatggtg ccgcgcggat
     4921 agatcagacc cgcgttgccg gtgaggaggt cgtacgcgag cgagccgtgg ttggtgacca
     4981 ccatgtcgag gttgttcacg ttcatccggc cggtattgtc gatcgtctgc gcggcgcgct
     5041 gcgccttggt cgcgcggtgt gacggcacgt aaaccacccg cgtcgccgcc aggacgcccg
     5101 gagcggccag cagcgtcaga agcgccgccg ccccgatcca cttcagggat ttcatccgtt
     5161 gggccctcct tagcctcggt cccatgtcag aaattcagct tggcgccaaa ccgcaccatc
     5221 cggggaacgc cgtgcaggag gggcgactgc tcggccagct gatagcgctg gatggctcca
     5281 tccgtgccgt acttcgaggc gttgccttcg atgaaggcct gcccttccgc cgtctccagc
     5341 cagttggtcg tgacggccga cccggaagag gtgtagacgg tgtacacgtt gtcgcgattg
     5401 aagatgttga gcgcccagat gtagagctcc agattctgcc gcccgatggc gatggtcttg
     5461 cccatcttgg cgtcgacctg gaacgtccag gggccgtagc gcgcgtccac cgggccgctc
     5521 ggctcgctcg ccgtgttggc cagggtcacc tcgttgtaga ccttcgtggg cgtgtagggc
     5581 gtgccgctcg aggcgttcgc gaggaggttg atgcccgcgt tctcgaggat gtggttgccg
     5641 tgccacgtcg ggccctgtcc cttgtcatag cggaaatcga agttcgccga cagcttgtgg
     5701 cgctgatcga aggcgagcgg cgaggacatc ttcggcgttt cggccgcgta ccaggcgatg
     5761 ttgcgctgcg tctgcgacaa ggatcccgtg ccgcgcgccc agctcaagct gtacgaggcg
     5821 ttgaacgaca ccagtcccgt gcgccgcagc gtgtacgaga ggtcgagccc cttgatcgtg
     5881 gcgaagtcgc gattccggta ggaggagaac gcgttcggcg ccgaggggat gttcgtgatc
     5941 tcgaccaggt ccttcacgtc cttgtagtac gccgacgcct cgacccgcga gcggtcgttc
     6001 ggcgtgtgct ggatccccag ctcgtacgcc gtcgtctgct cgggcttcag gttcgggttg
     6061 ccgaacccga cgaagtagcc gccggtccgg atcttgtgct ccaggaaagc gtacgagacg
     6121 tagagatcct gaagattcgg ctgctggaag aacttgccgt agttcacgtg gagcagcgtc
     6181 tggtcgctga ccgggaagcc cacgcccagc cgcggcgaga tgcgctgata gaccttcgac
     6241 ttctggaggt cggtggcctg gagccgcgaa tcggtgccgg tcgcgtccgg gtccagcggg
     6301 agcgtctcgc tccggagcgc gtcggtcgac ggggtcaggt agtcccacct gagcccggtg
     6361 ttcagcacca ggcccaggcg ctcgtacttg ccctgcacgt acagcgaggc gaccttgggg
     6421 tgcttcggca cgtcgagcgg gctgtcgtag agcccgtttc cattcttgtc gctgaagggc
     6481 tcgccgacgt cgtagctccc gttgccgttc gtgtccgtga acgtgtcccc ctcgttcagc
     6541 tcgttcccca gcgcgtcgta gccgtagcgg tccacgtccc gggtgttgtc cgggttgccg
     6601 gccgcgtcat acagctgcgt cgggaagtag tggtcgtaat agcggagggt gtgccgctgg
     6661 tagtcgccgc ccgccttcag ctggaaggag cgggtcagct gattcgcgta gttcgcggcg
     6721 aacccgacgt aggaggactt ccgatggagg aagtcgtcgt acacgtgggg gcccgtcgcg
     6781 ctggcgtcgc cgtaccagaa gagcgcttcc gactggtcgt acgacgggtt gccgttgggc
     6841 cgggagtagc cccgcagatc gtcgaacgcg acgccgtcgc cgcgcatgcg ctcggtgagg
     6901 aagtagttcg ccttcacctc gtagtaggcg cggtccgaga ggctctggtt ccacgtcccg
     6961 tagaccgagc ggttcttgtc ctcgtaccga ggcgcgtggc gcaggtcgaa ccggtagctg
     7021 ttcaggtact gctgccagtt ctcgtcgttc agcgtcccgc cgatcttcag cgtgtggagc
     7081 ggcgagggct tgatcgtgag cttgccgacc gtggcccagg aggcgctgga gttggccgga
     7141 aggatgccct tctcgaagaa gtcggtcgag atctcaccct gcggcgcgta cgcgaccttg
     7201 tcggtctcga agctggggtt gcggtcccgg ttgaagcggc gctcgccgga caggtagaag
     7261 ttcacctgct tcaggctggg cacgatcggg cccccgagcg aaagcgcgta ggtgttgttg
     7321 tcccggcgcg gggcgctgat ccagcttccg gccaggttgt cggtgacgcc ctcgatcgag
     7381 ccgaagtact tgtccgagcc ctcgcgggtc acgacgttca cgacgcccga gttgatgcgg
     7441 ccatactcgg cgttgaagcc gcccgactgc accacgatct cgctgaccgc gtcgttgttg
     7501 atcgcggtcg tcgagaagcc ggtcagcggg tcctgctgcg agaagccatc cacgtagtag
     7561 gcgacctcgt tcgggcggcc gccgcggatg atcagagtgc tcccgttcaa tgactcgttt
     7621 ccgttgagct ggggattgtc gaggcgggcc tggaagttca cgacgcccga ttgctgggcc
     7681 accgcgtcct tgtagccgcg gagcggctgg ttgcggatct ggtccccttc caggaacttc
     7741 gtggtgccgg tgacgtcctt ctggatgagg ggacgctcgg ccttcacggt cacttcggcc
     7801 acatccttga ggaccgtgga ttccagcgac cagtcgagct tggcgttgaa gtcgggaatg
     7861 acctccacgc cctctcgcgc ctgcggcacg aagccgagga gcgccacccg gaccgtgtac
     7921 ttgccgggcg gcaggttgag gatgaagtag ttcccggtgg cgtcggtgac ggcgcctcgc
     7981 ttcaggtcgg gaatcgtgac gctggcgagc ccgatcggct caccggtctt cccgtcgcgc
     8041 acggtgccgg agatctggcc cgtggtaccc gcccgcgcgt ccggagcaac aaaaaaggcg
     8101 cccaccacga tgaatgtggc gacgccgagc gacaacagcg tccgaggtcg aaccatgact
     8161 cgccctccta tgaatctcgt gcgtggaaac gccaagcggg gttcaccctc cctgatcttt
     8221 acagaccacg cgcggcacac ctgtaggcgg ccgccgaatc cctcgccctt cactgggccc
     8281 ctcatcggca ttcgtgcacc ctaaccggcg ttttttctct aggcagcctc ctccgccttg
     8341 agtcgtctct caatcggccc cggggtttgc ttgggccaca acaccttgcg tgacttcaac
     8401 actaggaatg tgaagcaacc gtgtcaataa tttttcggaa cttctccggg atccgcagaa
     8461 acgaaagggc ccccggcgat tcgccggggg ccccatgcag cggccggagc ggtcgcccgc
     8521 gccgggtcgt ttctagaagt ggtacgtcgc ggaaacgcgg ttgaacggcg tcgcctcgtt
     8581 ccccgacagg atccagccgc cggagagcgg gaactggttg ttgatcgcca tgtcgatgtc
     8641 gagattgttc cagcggatac cggcgccgag gccgagattg aagtcggcca gactctcctt
     8701 ggtgagatcc gtgttggacg gcgtgttgaa gtccgcggac tcggtccgct cgaccaggtg
     8761 cccgcggctc gcgccgatcc gaatcttgag ccagctcgtc gcctcagtct cgatggcggc
     8821 gaagacgagc ggaagcatcg tcgacttgtc ctcaaacgtg tgcagctcgg ccagggctcc
     8881 gccggtgttc tgatcggtcc gctcgtactc gtgcttgaac tggccgaccg aaacgcccca
     8941 gaggagcagg ttgttgtcgt tgacccgcat gttgtccgag atgccgaggc catagttctt
     9001 gtaggtctcg tcggctgaaa ccgtcggccg cgcgccaaga ccggtcatct cccacgagag
     9061 atcgtcattc acgtaccagg cggccgggta ccaggtgtgc gtgccgccgc gatgcatgat
     9121 gaggcgggcg aaggccccgt acgaggcgtt gccctggtcc tgccagctct cgccgcccgc
     9181 gggcgtctgc gtgcgatcca gcgtgtagcg ccggtaggtc gcggccgcct cgaagcggtc
     9241 atcctctcca aagtgcagcg cgaaggacgg ggtgatgccg tacgtgttga gctcgatccc
     9301 gttgcccagg atcgcgtccg gggcgacgtc gacgaagaag ggaatgaccc cgaacccagg
     9361 atcgaatggg ctgttgccgt gcagctcgat ggaggccgga gcgttcgtgg attcctcaaa
     9421 cttggagttg gtgatgtcca accggacggc aaagtcgaag ttcgagaacg cgcggcccca
     9481 gatcatgtcg agctgctcgt tgttcaggtt gccggagccc gtgagcacgt ccgtctggct
     9541 ctgcgccatc tggaacgcga tcgctccgat ccgcgggaag ttccggatga ccgtgtacgt
     9601 ctggtcggtc acgtcgaccg gattcgcttc gccgtcgttc gggttctggc cgagctccgc
     9661 cagaacgagg ttgttctgcc ggacgaccga cgtcggatac gtgtagatgt tggcgtagtc
     9721 gtcggtgaac tggctcagca gtggaacgtc gaggcccatg ccttccatgc gggaaagcga
     9781 ggcctggctg acgccggcgc tgatcgcgag aaggaccgcg atcgtgaggg aaacgatcag
     9841 cttgcgctga gacatcgggt tttgcctcct gtaggtagtg gaccccagac catccagggt
     9901 ccccatcccg gaccgtagcg ctctactgct gctggaaact ggtccgggga gaatacgaga
     9961 gagcaggtga tcctgttcga ctctcgctcc ccaccccctt tccatgtctc tagccacaca
    10021 ccgttaggcc gccagggcga cggccacacc acaccccgag agcacgtcct gagaggcttt
    10081 tccgagatcg gcgggacctt aggggcgccg ttccagcgtg tcaaacgaat aattcccaat
    10141 taagagctaa gctgcttcaa taaaacgggt tatatggctg gcgcccggtg cggaaagtac
    10201 gtaggcggag cggcgatgga cgctgcgcca ggtcagagcg ccgaggagcg tggatcgagg
    10261 tcgtatcggc cgaattcgta cttgtcgacg aagtagaagg tcttccgctg ctgatagtag
    10321 taccagatct cctcggggtg cttgtcgatg gcgaacgggc ttcgcacgac ctcgtcgggc
    10381 tggccgtact tgatgtagac cttccccatg tcggtcttcc atccgggccc cgccacgccg
    10441 aagtgctgat cggcgtaagc gatccggcgg tagaactcga ccatggactc gttctcgggc
    10501 gtgtccggag tcgggtcgcg ccgtttccag aacgcttccc agaactcccg cttctgctcc
    10561 gtggtcttcg ccgcgtccat ctccttcttc tcgtccgagt cggcgatgta cgccaggatc
    10621 tccagggtgg tcttgggatc ggccacgatg gtggccgccg actggacgac ctcgaacgac
    10681 ttctccctgc ggatcggctg gcgcttcccc ccaggttgcg gcaggagggg cgccttgagc
    10741 tccacgagga accggtagtt tccgggcacg agcgcctcgg cggacgggcg cagcaggaag
    10801 ggcgtgcgcg cccccgccct ggcgagggtc gtatccctct ccaagacgcg ctcctgctga
    10861 tcgttgagga tgcggtactg gatctggtag ccgggatcga agcccgccgc gggtcgcgag
    10921 tccaccacct ctccgtacac ggccagggtc gtgacgtcct cgctgtagcg gcgcgcggga
    10981 ttcggaacga gcgccgcgcg cgggtcgctc ccgggcggga cgcccccctt cacgtttccg
    11041 aaggagaggt cggtgaaccc gatcggcccc gcggcctgct tctgaacctc cagatcgatg
    11101 cgggtgcccg aggcgcgctc ggcgaccagg tcgcggatca ccacctggac ctcgtacttg
    11161 tcgggcggag cgggaaggtc gacgtggtcg atgatgtcgt tcccgatcgg acgggtctcg
    11221 gcgtaggtct tcacgtcgat gtcgcgcacg aaggtgtccc cggtgacttg gcggcccccc
    11281 ttctccttat agaagacgac gctcacctcg taggaggcgt ggtagccgcc gggacggcgc
    11341 tcgaagagga gctcggcgcg ggccaggcgg tagtccagcc gcaccagggg cacgcctccg
    11401 tccccgggct ccacggtgcc gtccacggag aagcgcggcc ggtcgccgct cgtgcccggc
    11461 acgggggtct ccggcgtggt gggcgggctc gggttcgggt actgcgccca ggcgcgcgag
    11521 ggggccagcc cggggagtac cggcgccggg gccaaggcca gcgccgcgag gagtaccgcg
    11581 gcgatgtgcc gcggatcagc ggagcaggat gatcgcacga gaatcgtcca gaccggctcc
    11641 ggtgagccgg agatagtaga cgccgctggg ggctgcgccg ccgtttcgat cgcggccgtc
    11701 ccacgtcatg atcccgaaac cggagaccgg ctgagccggg gcgcgcgatt cgcgcacgac
    11761 ccgtccggag acgtcgaaga cgcgcagcgt gagcggcgcg ccggccgacc cgttcggaac
    11821 ccggtaggac aggctggcta cggcgccggg ccgggcgcgg aaggggttgg gataggcgcg
    11881 gccgacgagc ggcgccgagg ccaccgagag cgttccctcc gcgaccttga ccgagctccc
    11941 gtcggcgaag aacgcctcga gccggtagcg cagggcgcct ccggcgatca gcgaatcgtc
    12001 gacggcggag acggcgccgg gcggcaggag atcgcggttc acgcgacgac ccgcgggatc
    12061 ggacgctgtg ccggaggcgg tcacgagatc gcggtagaga ttcagcccga ccattcccgc
    12121 gcggacgacg ggcgtccacg tgaccagcgc gcgcggcgtc gccgaagccg gatccagccg
    12181 gacgtccagg acgggaacga ccgtgggcgc cgggctcacg agatagcgct cgaggccgag
    12241 cgaagcggcg acaaggagcg tatcccccgt ccaccacccg tcccgggcga cgccgggcgg
    12301 aaggatcgga ccccgcgtga ccggcgccga tgggacgccg acgtccacga cgagagcccc
    12361 caggtcgccc gcgaacacca ccgcggaggt gccgttccgc gccacgccca ggaggggcgg
    12421 cgtggccagc tccgcgcgca ggaccggctg cgccgggttg gccacgtcca cgatctggag
    12481 cccgcgggtt cgcgtcgcca cgaggagcag cgtgcccgag aggtccacgt cccgcgcgcc
    12541 cgcgagcgac gtggttccga aggcggccgg ctggtcggga ttgccgaagc cgatggagag
    12601 gaggccgagc gactcgtccg ccacgaagag caccgtgtcc cgcgccgcga gcccgcgagg
    12661 atcctggacg aaggggttcc ccaccgagcc ccgtgcgtta agcgtgacgg tggaatcgag
    12721 cggctggtaa tccacgcgaa cgaacgtgac cgcgggattc aatccgcgcg tggccaccgc
    12781 gacgagattc gggccgacct gggtcaccgc gctcgcggag ccggccagcg tcagcgtcgc
    12841 gacgggaatc ggcgcggccg ggtctccgac ctggacgacc tcgaggccgg ccgggccgct
    12901 cgccaggtaa acgagcggaa gggcgggatc caccgccacg tccaccggtc gcgcgttgcc
    12961 cgacggaggc gccaccccga ccaggctgga atcgccgccg ccggcgcgat agatccgaag
    13021 cccgccggag tagttcgcga cgtaggccag cccgttcttc accgccaccc tgcgaacccg
    13081 gccggtggcc ccgaccggcg cgcgatcggg cgcgcccgac agggagacga gctcgagcag
    13141 cgaccgaccc ggctcgtcgg gaggcgcggg agcgatctgg tcgtaggtcg aagccacgat
    13201 ccagtcgccg ctcaagcggg cataggcggg gtttcccgtc agcacgcgat cgatctcgcc
    13261 ggtcagcgta tcgccggccg ctccgacccg cgcgaatcgg gcgagcgtct tcccttcggt
    13321 tgccgcgacg accacggaat cgttcgcgtc caccgaccgg acgaactggg tcccctgcac
    13381 cggggtcacg ctgcgcaggg cgtgcgcggc cacgtcgatg gcgaggacgc ccaggttccc
    13441 gtcggccacg aacgcgacgg ctccgaccca cgcgaggtcg tcggcgcggt gcccgttgaa
    13501 ggcgaactcc tccagcgagt cgggagccgc cgcgccggcc cgcatgcggt agagcgtcag
    13561 gatgagccgc gtgggcaagg tgccgcggac gctggccacc aggaaggaat cgccccgcgc
    13621 ggccacggcg cccaccgtcc gcccgtcgct gtagacgagc gtccggcgaa gcgtgaatcc
    13681 cgacgccgcg tcccacgaca ggaaatggag ggtggatccg cctcccgcga ccacgccccc
    13741 gcccgaggac gcggcggctc ccagcgtcac ggttcccagg tctcccgcgg atcccgcggc
    13801 gacgggattt gccgggttgg agacgtccac ggcgaccacg ccgcgcggtc cggcgaagag
    13861 gaagagggtc gagcccgcga cggacgcccg ggcgacgttg cccgccgtgc ggaagcgtcc
    13921 gaggagtggc gtcgagggct ggctcaggtc gtagagatcg gcgccgctcc cggtcggcac
    13981 ggcgatgacg ccggccccga ccgtcacggc cggaccgaac gaggccgcga gggacgaagg
    14041 gacgccgcga agagcgaccg tctcgggaat ggcatgcgcg gcgggagggg ccgcgacgag
    14101 aaggctgccc accgcggcga gttgcgccca ggagcgccgg cgtcgaagcc tgcgggagtt
    14161 catctgatag gacgctacca tgcgctggtg cggcgctcca agacggaact ctttctcctt
    14221 gtcgccgcgt gatcctctcg gctacggtgc cgccgtgccg tccctcgaca cgatcctcgc
    14281 accgctctcc acgttcatcc tgtcgaccat ctcctcatgg ggctacgcgg ggatcgtcgc
    14341 ctgcatgacg atcgagagcg cctgcatccc gctccccagc gagatcatca tgcccttctc
    14401 cggctatctc gcctccaccg ggcgtttcac gctctggggc gtcgcgctcg ccggcggcgt
    14461 ggggaacgtg ttcggctccg cgatcgccta cgccgcgggc cgctacggcg gccgcccgct
    14521 ggccgagcgg ctcgcccgct ggcgggtgct ccgcatgtcc gactacgacc gggggaatct
    14581 ctggctccag cgccacggcg tcgccgtcgc gttctggagc cggctccttc ccatcgtgcg
    14641 cacgttcatc tccttccccg cgggcgccgc acgcgttcct ttcggcatct tctccctgta
    14701 caccttcatc ggctcggtga tctgggcgat ggccctggcc tgggtcggcg tgctcctcgg
    14761 cgagcactgg cacgacatcc ggaaggtgat gcgcgggttc gacctcgtcg tcgtcgctgc
    14821 gatcctcatg gcgggcgtca tctgggcgcg ccaccacttc cgcgccgatc cggggggccc
    14881 ggccccggag tagagcgggc ggtcgcggcc ggttgacaac gcccgctccc cggccctagg
    14941 ttcgcgcctc aacccttttc cagcgtcgcc ggtccaacca cccgtggggg gaatgacggc
    15001 gtcggacaat ggtatggggt tcggcgtgac gttccatttc ggggagttcc attgaggccg
    15061 gccgaacccg ccggcgcgtt gacggtccct cgcttcacat gataaattca ccggctcttt
    15121 gggggatcgt ccagtggtag gacacgtggc tctggaccac ggtacagggg ttcgaatcct
    15181 cttcccccag ccatgccaga aacgggacgg tacacccatg aagccggttg cggcgctatc
    15241 gtctaggggt taggacgggt ggttctcagc catcaaaccg gggttcgaat ccccgtagcg
    15301 ctaccaacac gatcccatgc gccgcggcgc ctgcgggccc acgggccgcg gctcgccgcg
    15361 agcgccggga tcttgctcct cctcttcggc cttctcgctg cagcggcgcc cgtggccggc
    15421 gcccagcccc gccccaccca ctcgcgcgcg tcccgcgcgc tcggtaacgt cgaacggatc
    15481 gattccctgg gcggcatcac cgagtaccgg ctccgctcca acgggatgaa gatcctgctc
    15541 tcgcccaacc acgccgcgcc cgtcatcacc ttcatggtcg tctatcacgt cggctcgcgg
    15601 aacgaggcgc ccgggaacac cgggtcggcc catctcctcg agcacatgct cttcaacaag
    15661 agcacgaaga acttcggcaa ggcgaaccac aagaagacgg tccaggaggt gctgtacgag
    15721 tcgggggcgg actacgcctc ctcgaacatg accacctggt acgaccgcat gaacggctac
    15781 tccacgctcc ccgcggagaa gctcgagacg gcgatgaaga tcgaggccga ccggctgggg
    15841 cgcgcgctga tcctcgattc ggagcggcag ccggagatgt cggtcgtccg gaacgagtac
    15901 gagatcggcg agaacagccc ctatcgcgcg ctcgacaagg cggtgatcgc cgaggcgatc
    15961 caggcgcacc cctatcactg gaacacgatc ggataccgct ccgacatcga gggggtcacg
    16021 acggagaagc tgcgggagca ctaccgcgac ttcttctatc ccgacaacgc cgaggcgatc
    16081 ctcgtgggcg acttcgacac ggcggccgcg ctcgcgatgt tcgatcggga gttcgcggcg
    16141 ttcgcccggt cgaagacacc gatcccgcag gtgatcaccg tcgagccgcc gcaggaaggc
    16201 gagcgtcgcg tcacggtgcg ccggccgggc caggtcggga tcgtcgagat cgcctacatg
    16261 cggccgaacg cgctggaccc cgactacgtg ccgctggacg tgctctcgtc gatcctcacg
    16321 gccggcgtga attcgaggct gtaccaggcc ctcgtcgagc cgggcctcgc gaccgacgtc
    16381 gactcctgga actacacgct tcgcgatccc taccccatcg tcgcggaagc gacggtggcg
    16441 ccggggcgct cccacgacga cgtggagcgc gcgatgaagg ccgctctgta ccggatcggc
    16501 aaggaaggag tgagcgcgac ggagctggac cgggcgaaga gccagctcga ggtctccgtg
    16561 gtgcgcggac gcgacggcac ctacgagctg gcgtcgtccc tgggcgaagc ggtggcgtcg
    16621 gcgaactgga agtggttcgt gggctacgtg gacgcggtcc gtagcgtctc ggcggacgac
    16681 gtgaagcgcg tcgcggcgaa gtacctcgtg cccgaccacg cgacggtggg ctggttcgtg
    16741 ccggcgtccg gcgagaagac ggaggcaaag gtggggctcg cccccgcgcc cgtccgggac
    16801 aagcgggccg gcgccggagg cgcctcggaa gcggcggcgt caggattcgg ctcgcgcacg
    16861 ctccacaagg tcctgggcaa cggcctcacg ctggacgtcg tggtgaaccg cgccgtgccg
    16921 acggtcgcga tccagggcac cgtgttcgcg ggccggatgg aggcgccggg cggcaaaccc
    16981 gcggtgcccg acctgaccgc catcatgctg acgcgcggca cgaaacgcgc ggacaagcgg
    17041 gtcatcgccg cgcggctcga cggcgtcggc gcgcagctcc agatcttcgc cggcgtgacc
    17101 gaggccacca tcgtcggcaa ttcgctctcc cgcgacctgc gacttcttct cgggacgctg
    17161 gcggacgagc tcgcgaaccc cgcgttcgcc gattccgagg tcgcgaaggc caagctggag
    17221 atgcgcgcca acgtcctgcg cagctcggag agcaccgcgg ctcggggact ggaccggctg
    17281 acccgcctcg cctaccccga gggccatccc taccgggcgg cgaccaccga cgcgatgctc
    17341 gcgagcctcg acaaggcgaa cgccgccgat ctccgcgcct tctggcgcgc gcgctatggc
    17401 ggggccggaa tcatcctggc ggtggtcggc gacgtggatc cccaggccgt cgccgccctg
    17461 gccgattccc tcttcggggg gatcgagcgt ggcgcgcgtc ccacgcacga cgcggccccc
    17521 acggcgccca acgcccccga gcatgtcgtc gagacgatcc gcggcaaggc gaacgtcgat
    17581 ttcctctacg ggcaggcgag cgggctcaag cggaacgatc ccgactacga ggccgccctc
    17641 gtcgccaacg ccgcgctggg acaatccagc ttgacctcgc gcatcggcaa gcgggtccgc
    17701 gacaccgagg gactctccta caacctctac tcgcgctggt tctggcccga ctacgccgac
    17761 ggactctggg cggtggacgt cgccgtcgcg ccgcagaacg tggccaaggc gctccgctcc
    17821 accaaggaag agatcgatcg ctacgggaag gaagggatca ccgacgcgga agtggaggtc
    17881 cagcgggagt tcttcgcggg caactacctg gttcgcctgg gaacgaacgc cggggtggcc
    17941 gcggcgctct gcaccgcgga gaagttcggg tacggcccgg gctacctgga cgacttcccg
    18001 aaacggatcc gcgccgtcac gaaggcgcag gtggacgacg tgcttcgccg gcgcttccat
    18061 cccgacaaga tgcacctggt cgtcgcggga gacctggact cgattcccca gtaggtcggc
    18121 tccgccctcc tgctcttttc tacttcaaca gcgtcagggt ccgggttttt gtctcccccg
    18181 ccgcagtgag ccgcgcgtag taccggccgc tcgcgaccgg aagcccccga ttgtcccgtc
    18241 cgtcccaccg cgcctcgtag cgtcccggcg tcgcgggcgc atccaccagc gtgcgcacca
    18301 gcgctccgga gacattgtag aggcgcagcg cgacgcggcc cgaagtcccc agctcgaagg
    18361 gaatgaccgt gctcggattg aaggggttgg gccgattcgc cagcaggcgc gtcggaggcg
    18421 gagccggcgc aggcgccacc gcgctcacgc tcgggatcga cgcgggattg gcgggattgg
    18481 agccaagtga cagctcccag cggtcgcgat agccatcccg gtcccgatcg attcccatgc
    18541 gccgcccggc gcccggcggc accccgaggt acgtgatctc gccgccgtcc tgcgcgagga
    18601 ggcgcaggct gtcggcgggg aacgtcccct ccggatcgta gtcgctcagg aacgagcgcg
    18661 ttccggcttg atagtagaat cccttcgcgg caccgcccag acgcccgtgg gcgaccaggt
    18721 cgcattgccc ggcgtccgcc gccgcgtaca gggagtcgag gaactgcgtc gtcccggtca
    18781 gggtgcgatt ggctccgttc accgtgatct cgcgtcccac cgagggcgcg gtcccggtgt
    18841 cgaacgcgag gaggaatgcc tcgaggtcgc ggcgctcctg gttgttcgcg aacgtgaaga
    18901 ccggaaggtt gaggaagtcg aacaggttgt ccatggagcc atcgtgaagg aatccgaagc
    18961 cgcgcttttg aggaccggcc gcgtcggtga agccggtctt ctggtagaga ttccgcagct
    19021 gcggcacttt catcgcctga ctctcctgca acgcctgcgc cgggatcagc gttccgttgg
    19081 tccccgtcgg gaaggagtgg cagacgctgc aggtcccgcc gtcgtgcggc ttggtgtcga
    19141 actcgacctt accgcgcgtc gggctcggcg tcggcgctgc gggattcggc caggtgcgat
    19201 cgaggttttg attcggattg ggcggatagg caagcgtcag gatgaagtcg ttgaactgct
    19261 gcatctccgc cgcggtgagg gaatcgatgt tccccatcag cgagacgaac gcggggttga
    19321 accgggtgaa gtccgcgcgg tcgccgcgcc agtggaacgg cggcgtgttc gcgagtcccc
    19381 ggagtgactg cgtggtcatc gggcccttca tgggatgaaa gggcgggatg gggaccggat
    19441 tcgagggcgc ggcctgcagg tccccctggg gattcccgag atcccacgcg aggttgtcga
    19501 agttgccgcc gatgtggcag gaggcgcagg cgaggtctcc gtggtcggag agcatcccgt
    19561 catagaggaa gcggcgcccg gcggtgaccg ccggaggact gggatcgaaa cccatgccga
    19621 gcggcgtctc actctggaac gactcgccgg ctgtcccgat cacggccacc gagttcgaga
    19681 agcggttcag cacatacagc cgctgccgtg ccgcgtcgag ggcgagcgcc accgggccgc
    19741 tcttcgtgac cctgggagcg gccgataccg gaatgcgtgc cgtgaccaag ccggtcccgg
    19801 ggtccaccac accgacgaga tcggagccga gcgcggcgac gtacaccttg ctctcggccg
    19861 gattcacggc gacccccgtc ggctccgcca gactcagcgc cttttccccg gccggaccgg
    19921 gaatggtgtc gtacacgacg tgcgcgttca gatgccacgc cgtgaccgca cccagccccc
    19981 cggcgtcgat ccgcgtgagc cggttgcgca ggaagcggcc ccggagattc ggctcgaatc
    20041 gagtgcggtt ctgggcctcg gtgttcgaga cgtacaggtc gcccgtgccg gacgcagccg
    20101 cgacatcgaa caggttcgtc cccacgcccg ataccgtgcg gagcaccgcg ccgctcgcgg
    20161 cgtccaggac gacgacgtcc tggtcgggaa ggtcgtaggg gatgaacgtg ttccaggtct
    20221 tcacgctggc cggccgctcg tcgacccagt cgttgcccac cttctggacg atgaggccca
    20281 ccgccggagg cgcgggaagg cccgctttga gtggcggatt cggagggggc ggtccgccgc
    20341 ccgactggac ctcggtggcg ctcaacgtcg tcgtccggtt tcccgcgtcg agcacggcga
    20401 cgtagacctc gccgccccgt gccgcgagcg cgcgagggtg ccgtccgaat acagggcgga
    20461 ccaggtcgag cgtggtgccg gcctcgcccg ccaacgagta gaccttgatc gcgtcctcgc
    20521 cgctcacgca cacaaacgct cggcgagggc tgccggcgag cacgacgtcg gtcggctcgt
    20581 cccccacacg cagggtggcc gcgacccgct tcgagttcag gttcacgatg ctcacgtcgt
    20641 cggagacgtt gtccacgacc caggcgatgc tgtcgttcag cgcggccacg gagaccggct
    20701 ccagccccac ggggacctcc cccgcgctcg tgagccccgc atcgccgatc gtgaagatct
    20761 ccagacgcgc gtcgggcagg ttgcaggcga ggagccgcgt gccgtcgggg gtgagcgcca
    20821 gcggatggac cgggagcgac tcgaagttgg tgaaatcgga cagggccgga gtggaggagt
    20881 gaagcggcga caccggccag gccaggagca cgaggacggc cgcgcacgcg ccggccatga
    20941 gcgcggtgag gaagcggaat cgctgaaagc caccggtggg taacgacatc gggcccgaaa
    21001 cgagatcgcg agagaatggg aacgagtgca tcaagccgtt gacgcaggcg tgattatagc
    21061 atacttaact ccggcgcgca accggtcagc ttccgctcgg cggctctcgc atcaccgttc
    21121 ccctcctcga acgccgacca tgcctcgcgc aggagggcgt ccatccgctc gcgatagcgc
    21181 gcgcggtcga acgtctgggc atgacgccgc agccacgccg gatcgaacga gcgccgcgag
    21241 agcgactcga acgcggcttc aagtgccgcg tcgtcatccc cctcgtagag gatcccggtt
    21301 ccctcggcga ccacctccga ggcgccgccc cgcgcgagcg ccacggctgg ccgactgcac
    21361 gccatcgcct cgagcggcgc gatgccgaag tcctcctcgt gcgcctgcac gagcgcgcgg
    21421 cagccgcgat acgcttcgcg cagggcctcc gcgctcagcc agccgtcgaa gcgcacggtc
    21481 ggacccgcga ggcggcgaag gcgcgcctcc tcgggacccg ttcccacgat ccggagcggg
    21541 actcgcattc gattcaccac gcggatcgtc cgctccaccc ctttgtacgg cacgagggcg
    21601 gagacgatca ggaagggcgc gccggggtcc tgcgtcaggc cggacgcggg cgtgaagaac
    21661 gcggtgtcca cgggcgggta cacgaccgcc gccggacgcc cgtaatgccg cgcgatccgc
    21721 tcgcccacgt gcttcgaatc ggcgacgaag aggtgcacgc gttcggaact cgcccgatcc
    21781 cattcgcgga atcccggcgc cacgagcgct gcggccgcac gcgtgagcgg tcccgcgcga
    21841 tccggtccga agtactcgcc atagaggtcc cagaagtacc gaacaggcgt gaagcagtag
    21901 cagacgtgga gcgcgccggg ccgcgggatc acccccttgg ccgcgcagtg gctcaacgaa
    21961 atcacgaggt cgtaattgga gaggtcgaac gattccacgg cccgcggata gagcggcacg
    22021 aaccaacggt agcgggagcg cccgaacggg agccgcgcga tccacgactc cacgaccttt
    22081 cgccgctcga tcacgggggc gacgctgccc cgccggtgga
//
LOCUS       DASSLZ010000024         4314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1531065, whole genome shotgun sequence.
ACCESSION   DASSLZ010000024 DASSLZ010000000
VERSION     DASSLZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531065"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFM00_00855"
     CDS             <1..432
                     /locus_tag="VFM00_00855"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7496826.1"
                     /translation="TIGYGMRSGLRALREGSFLGMLPDQDARKAGIHVPFFGRPASTH
                     TGPARLAARTGAPIGVAVIERTGRGRFRARLRRVLVPDPRAPEEAEVERLTREMTAEI
                     EAAIRELPGHWYWIHRRWKTPPPDPSGSPATAPAVTTPATT"
     gene            complement(422..739)
                     /locus_tag="VFM00_00860"
     CDS             complement(422..739)
                     /locus_tag="VFM00_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HET7496827.1"
                     /translation="MDAKKPPQPAGPPTQINVELGEKEAEGIYSNFVVIGHSLSEFVL
                     DFARVLPGTPKSKIFARIVMTPPNVRGLLAALEDNVRKYEGQFGKIPTMNPEASKGIG
                     FTS"
     gene            881..3133
                     /locus_tag="VFM00_00865"
     CDS             881..3133
                     /locus_tag="VFM00_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7496828.1"
                     /translation="MGSKRRTGGGRSEVDATADAVIIAEWKRFAARKLGSSDRVVEGG
                     CFLDAPPCPFDGRSSFEERFATDCVRCVRLRRAAAQVETSPPEGAGAIPTLGLLLRLH
                     EVSQGSGSGAGAPPPSSEDDGSRFGAASILLRALPMLHAASGLRRTARVLAASIAGAF
                     SDVIDTVLFFDALGEANTLRLECAFRRADVGIPVGDRDGFLDVELLAAGGAFDGEVFD
                     RLREMPIPLDQDRDLLSDAVFEGRTTVVAHPAREFRLPTLLVDHLPDAPAAIIPVFGR
                     ERVRGILVVSAAPGIGGFTSDQMELLMAAATQAGMALEGASLLDLARRRGGGLHAIYD
                     LLASLLKHAGGEPSLDASLHALADVTESSHGLAWARSADGVLGLTAVVGRPEAGESEL
                     REMGETFRHWFEADPKPMLLDRIGADPRFAGGVPEGWGSALAVPLRHEDRLWGVFLLV
                     REMRAGAEPSALPYDLEDAALGILVGAVASLARTREAGVESVHRAERRLRDAESQLRH
                     VEKVAVVGERGIQIAQEIRNPVAAISGFAKRVLQALPEGDENREYLEIILREADRLER
                     VLAEQIALAQLTRPRLKLQSLNTVVQEALELSSEDLVRRRVRLLKRLGPDLPSLLIDP
                     DKIRQVLSNVLRYALQAVPSGGRVRVETRSGVGVVQAEIAHDGPKVAGESLDRLFVPF
                     STSRRYGAGVGLAVAYQMVREHGGEIRARSEGDWSSIVTIYLPVRENQDRRARPDRRA
                     GRNDRRRRMA"
     gene            3199..3274
                     /locus_tag="VFM00_00870"
     tRNA            3199..3274
                     /locus_tag="VFM00_00870"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3232..3234,aa:Phe,seq:gaa)
     gene            3334..3409
                     /locus_tag="VFM00_00875"
     tRNA            3334..3409
                     /locus_tag="VFM00_00875"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3367..3369,aa:Thr,seq:cgt)
     gene            3581..>4314
                     /locus_tag="VFM00_00880"
     CDS             3581..>4314
                     /locus_tag="VFM00_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET7496829.1"
                     /translation="MTSDPLRGLMVSASGVRGIVGERLTPEVVTRVAAAHASMLQPGP
                     IVVGRDSRPSGAWVLRAAEAALLASGRDVIDVGIAPTPTILFAIQHHGAAGGLAITAS
                     HNPAPWNALKLFGPGGTFLAPAAAEAVARRAREGGFAWVTAERVGSIEEDQDAITRHR
                     DAILALPGFDLAQIRARRFRVAVDCVNGAGSIATPTLLRALGCEVTPIHCTPDGRFPR
                     VPEPLPENLGDLGAAVRSAGAAIGFA"
BASE COUNT          564 a         1576 c         1528 g          646 t
ORIGIN      
        1 accatcggct acggcatgcg gagcgggctg cgcgcgctcc gcgagggctc gttcctcgga
       61 atgctccccg accaggacgc ccgcaaggcg gggatccacg tgcccttctt cggacgcccg
      121 gcctccacgc acaccggtcc ggcgcgcctt gccgcgcgca ccggcgcgcc gatcggcgtg
      181 gcggtgatcg agcgcacggg gcgcgggcgg ttccgcgcgc ggctccgccg cgtgctcgtc
      241 cccgatccgc gcgccccgga agaagccgag gtggagcggc tgacgcggga gatgaccgcg
      301 gagatcgagg cagcgatccg ggaactgccc ggccactggt actggatcca ccgccggtgg
      361 aagacgccgc cgcccgaccc gagtgggtcg cccgccacgg cgccggccgt cacgacgccc
      421 gctacgacgt gaacccgatc cccttcgacg cctcggggtt catcgtagga atctttccga
      481 actggccttc gtacttccgc acgttgtcct cgagcgcggc caacagtccg cgcacgttcg
      541 gcggggtcat gacgatccgg gcgaagatct tcgacttggg cgttcccggc agcacgcggg
      601 cgaagtcgag cacgaattcc gacagagagt ggccgatcac cacgaaattc gagtagatcc
      661 cctccgcctc cttttccccc aactccacgt taatctgcgt cggcggcccg gccggctgcg
      721 gcggcttctt cgcgtccatt tgcaaccctc ctgttgacaa gcaattagcg gcacgtatcg
      781 gcctcaagat aagtccccgg gtgccgtaaa tcaacctgta ggtgtgccct gggtccgccg
      841 ttcggcggcg ccggcgaccg tgacaggacg ggaggacgtg atgggttcga agcggcggac
      901 gggcggcggc agaagcgagg tggacgcgac agccgatgcg gtgatcatcg ccgagtggaa
      961 gcgcttcgcc gcgcggaagc tcggatcgtc cgaccgggtc gtcgagggag gctgcttcct
     1021 ggacgccccg ccatgcccct tcgacgggcg ctcgtcgttc gaggagcgct tcgccaccga
     1081 ctgcgtccgg tgcgtgcgcc tgcggcgcgc cgccgctcag gtcgaaacgt ccccgccgga
     1141 aggcgcgggc gcgatcccca cgctgggcct tctccttcgc ctccacgagg tgtcgcaagg
     1201 gtcgggctcg ggtgccggtg cgcctccgcc ttcttccgag gacgacgggt cgcgcttcgg
     1261 cgcggcctcg atcctcctgc gcgcgcttcc gatgctgcac gccgcctcgg ggctccgccg
     1321 caccgcccgc gtgctcgccg cctccatcgc cggagccttc tcggacgtga tcgacaccgt
     1381 gctctttttc gacgcgctcg gcgaagccaa cacgctccgc ctggagtgcg cgttccgccg
     1441 cgccgacgtc gggatcccgg tcggagaccg ggacggattc ctcgacgtgg agctgctcgc
     1501 cgccggcggg gccttcgacg gcgaggtgtt cgatcgcctg cgcgagatgc cgatcccgct
     1561 ggatcaggac cgcgacctgc tctccgacgc cgtgttcgag gggcgcacca ccgtcgtcgc
     1621 gcatccggcg cgcgagttcc ggctgccgac gctcctcgtg gaccatctac ccgacgcccc
     1681 cgccgcgatc atccccgtct tcggccggga gcgcgtgcgc gggatcctcg tcgtgtccgc
     1741 ggcaccgggg atcggcggct tcacgtccga ccagatggag ctcttgatgg cggccgccac
     1801 ccaggcgggg atggcgctcg agggcgcctc gctgctcgac ctggcccggc gccgcggcgg
     1861 cggattgcac gcgatctacg atctcctggc ctcgctcctg aagcacgcgg gcggcgagcc
     1921 cagcctggac gcctcgctgc acgccctggc cgacgtgacc gagtcgagcc acgggctggc
     1981 gtgggcccgc tccgcggacg gggtcctggg gctcaccgcc gtcgtgggcc gcccggaggc
     2041 cggagagagc gagctgcggg agatggggga gaccttccgc cattggttcg aggccgaccc
     2101 caagccgatg ctcctcgacc gcatcggggc cgatccgcgc ttcgccggag gggtgcccga
     2161 gggatggggc tccgcgctgg cggtgccgct gcgccacgag gaccggctct ggggcgtgtt
     2221 cctgctggtc cgcgagatgc gggcgggcgc cgagccgagc gcgctcccgt acgatctgga
     2281 ggacgcggcc ctggggatcc tggtcggggc cgtcgcctcg ctcgcgcgca cgcgcgaggc
     2341 gggcgtcgaa tcggtgcatc gcgccgagcg gcggctgcgc gacgccgagt cgcagcttcg
     2401 ccacgtggag aaggtagcgg tggtggggga gcgcggcatc cagatcgccc aggagatccg
     2461 gaacccggtc gcggccatct ccggcttcgc caagcgcgtg ctgcaggcgc tgcccgaggg
     2521 ggacgagaac cgcgagtacc tcgagatcat cctgcgcgag gccgaccgcc tcgagcgcgt
     2581 tcttgccgag cagatcgcgc tggcgcagct cacgcgcccg cgtctcaagc tgcagagtct
     2641 gaatacggtc gtccaggagg cgctcgagct ctcctcggag gacctggtgc gccgccgcgt
     2701 tcgtctgttg aagcgactcg ggccggatct cccgagcctc ctcatcgatc ccgacaagat
     2761 ccgccaggtg ctttcgaacg tgctccgcta cgcgctgcag gcggtgccga gcggcggccg
     2821 cgtgcgcgtc gagacgcgga gcggcgtcgg cgtggtgcag gccgagatcg cgcacgacgg
     2881 gccgaaagtg gcgggagagt cgctggaccg cctgttcgtg cccttctcga cctcgcggcg
     2941 ctacggcgcg ggggtggggc tggccgtggc ctaccagatg gtgcgggagc acggcgggga
     3001 gatccgggcg cggagcgagg gggactggag cagcatcgtg acgatctacc tgccggtgcg
     3061 cgagaatcag gaccgccgcg cccgtcccga ccgccgcgcc ggccgcaacg accggcgccg
     3121 ccgcatggcc tgaggggttc ctacggcccc gggccgttga cccttagccc gcgcccatgc
     3181 taaagttccc gaccggctgc gaccctagct cagttggtag agcaccacac tgaaaatgtg
     3241 ggtgtccgca gttcgattct gcggggtcgc accactattt ttttcgcgaa gattcctccc
     3301 gcggcaaccc ttcgcgacga tgcaacgagt tcagctggtg tagctcagct ggtagagcaa
     3361 cgcactcgta atgcgtaggt caccggttcg attccggtca ccagctccat ctccgggtcg
     3421 gccggccctg accactcggc cgttcgcagg gattcagacc gcgacgccgc gcgccccccg
     3481 cacgggggcc gcggcgcacg cgtggggcga tccgccccga ggcttcggtc cgcgtggggc
     3541 ggagcgaagc cgcgcgaacc gctccccacg ggggaatcca gtgacgtccg atccgctgcg
     3601 gggcctcatg gtcagcgcct cgggcgttcg cgggatcgtg ggggagcgcc tcacgcccga
     3661 ggtcgtgacc cgcgtggccg cggcgcacgc ctccatgctc cagcccgggc cgatcgtcgt
     3721 gggccgcgat tcgcgtccca gcggcgcctg ggtccttcgc gccgccgagg cggcgctgct
     3781 cgcctcggga cgcgacgtga tcgacgtcgg catcgcgccc acgcccacca tcctcttcgc
     3841 gatccaacac catggcgccg cgggcggcct cgccatcacg gccagccaca atcccgcccc
     3901 ctggaacgcc ctcaagctgt tcggccccgg cgggaccttc cttgcgcccg ccgcggcgga
     3961 agccgtcgcc cgccgggcgc gcgagggggg attcgcatgg gtgacggccg agcgtgtcgg
     4021 gtcgatcgag gaggatcagg acgccatcac ccgccatcgc gacgccatcc tcgcccttcc
     4081 cggcttcgat ctcgcgcaga tccgcgcccg gcgcttccgc gtcgcggtgg actgcgtgaa
     4141 cggcgccggc tcgatcgcca cacccacgct gctccgggcg ctcggctgcg aggtgacgcc
     4201 gatccactgc acgcccgacg gccgcttccc gcgcgtcccc gagccgctgc ccgagaatct
     4261 cggcgacctg ggcgcggcgg tccgctcggc gggcgctgcc atcggcttcg ccaa
//
LOCUS       DASSLZ010000025         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1656892, whole genome shotgun sequence.
ACCESSION   DASSLZ010000025 DASSLZ010000000
VERSION     DASSLZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656892"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="VFM00_00885"
     CDS             complement(<1..97)
                     /locus_tag="VFM00_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7496830.1"
                     /translation="MPAITVDIEEQCVNTIKFLSADAVEKAKSGHP"
     gene            216..1694
                     /locus_tag="VFM00_00890"
     CDS             216..1694
                     /locus_tag="VFM00_00890"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET7496831.1"
                     /translation="MTPSRTIQVPIRILAALLLAVQAIGGCATNPVTGRKQISLVSEG
                     KELQMGAEADPAVISEYGLYSDQEVQRYVDGVGQKLGKVSHLPSLAWHFRVLDSPVVN
                     AFAIPGGYIYITRGILAYMNSEAQLAGVLGHEIGHVTARHSAQQITRQEIAGIGVLAG
                     AVFVSAFRPYSGVAQQGLGLLFLKYSRDNETQADELGVGYAAKAGYDPREIPGTYATL
                     KRIGERAGSSLPNFLSTHPDPGDREIRTTQLADAAAASAHRDLVIGAPVYKSRVDGIV
                     FGDDPRSGYFVGNRFYHPDLKFQLIFPQGWQTANQPSQVVAANQSAGAAMQLTLVKSE
                     DTSLSPSDYVASLQKKGEITQASGRSENFRDYPAWLGTVTAQGENGSQEFIACWARTK
                     PGEFLQILGQAKSQTAADQIFSSARSIAALTDPARINVTPDVVVIKPAPRSAPFSDLW
                     ATMGGGVLSLEDGAILNSTRPATVIPAGAPVKTVKKGTKPTS"
     gene            complement(2358..2933)
                     /locus_tag="VFM00_00895"
     CDS             complement(2358..2933)
                     /locus_tag="VFM00_00895"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET7496832.1"
                     /translation="MPKHPLLRATLLFVGIGAVIGVLGWLLRPHISHEALKQWVRHAG
                     AWGPVALLGVQAGQILAAPIPGVFVPLIAGVLYGPLWGSVLTAIGTAIGSTAAFAIGR
                     GAGRKVAERWIGEPALEQAQKLIGGKRWLALIPIFLFPFSPADALCFMSGIAGVAWPQ
                     FLIVVLFGRIPKDVVIAVGSALGWSFLGTHH"
     gene            3125..>3338
                     /locus_tag="VFM00_00900"
     CDS             3125..>3338
                     /locus_tag="VFM00_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496833.1"
                     /translation="MTDPFGARSTLAVDGRSYRIARLDALERAGLSLQRLPFSLRVLL
                     ENLLRRVDGKVVTAEDVEAVARWNPAA"
BASE COUNT          564 a         1167 c         1096 g          511 t
ORIGIN      
        1 cgggatgacc cgacttcgct ttctccacgg cgtccgcgga gaggaacttg atggtgttca
       61 cgcactgctc ttcgatatct accgtgatgg ccggcatggc tccctcgtcg cgtttcgttc
      121 ctcgcgcgcg ccgggtgatc ccgtcccgcc ccggcgccga cacggtgtat cataactctc
      181 tgttccgttg tacccctcta aggatggaga acgagatgac cccgagccga acgatacagg
      241 tcccgatccg aatccttgcc gccctccttc tcgccgttca ggcgatcggc gggtgcgcca
      301 ccaatcccgt cacgggcagg aaacagatct cgctggtctc cgaagggaaa gagctgcaga
      361 tgggggccga ggccgatccc gccgtgatct ccgagtacgg cctctattcg gaccaggaag
      421 tgcagcggta tgtggacggc gtgggtcaga agctgggcaa ggtctcccat ctgccctccc
      481 tcgcctggca tttccgcgtc ctcgactcgc cggtggtgaa cgccttcgcc atcccggggg
      541 gttacatcta catcacccgc ggcatcctcg cctacatgaa ctccgaggcg cagctcgccg
      601 gcgtgctcgg ccatgagatc ggccacgtca ccgcgcggca cagcgcccaa cagatcacgc
      661 gccaggagat cgccgggatc ggcgtcctgg cgggcgcggt cttcgtctcc gcgttccggc
      721 cctacagcgg cgtcgcccag caggggctgg gcctcctgtt cctcaaatac agccgcgaca
      781 acgagacgca ggcagacgag ctgggcgtcg gctacgcggc caaggcgggc tacgacccgc
      841 gggagatccc gggcacgtac gccaccctga agcggatcgg cgagcgggcc ggctcctcct
      901 tgccgaattt cctctcgacc catcccgatc cgggcgaccg cgagatccgg acgacacagc
      961 tcgcggacgc ggccgccgcc agcgcgcacc gcgatcttgt gatcggcgcg ccggtctaca
     1021 agagccgcgt ggacgggatc gttttcgggg acgacccgag gtcgggatat ttcgtcggga
     1081 accggtttta ccatcccgac ctcaagtttc agctgatctt tccgcagggc tggcagacgg
     1141 cgaaccagcc ctcccaggtc gtggccgcga accagtcggc cggagctgcc atgcagctca
     1201 cgctggtgaa gtccgaggac acctccctct ccccttccga ctacgtcgcg tcgcttcaga
     1261 agaaggggga gatcacgcag gccagcggcc ggagcgaaaa cttccgggac tatcccgcgt
     1321 ggctcgggac cgtgaccgcc cagggcgaga acggctccca ggagttcatc gcctgctggg
     1381 cgcggaccaa gccgggcgag ttcctccaga tcctgggaca ggccaagtcg cagacggcgg
     1441 ccgaccagat cttctcttcg gcgcggagca tcgccgcgct caccgacccc gcgcggatca
     1501 acgtgacgcc cgacgtggtc gtgatcaagc cggcgccgcg cagcgcgccc ttctccgatc
     1561 tctgggcgac catgggtggc ggcgtcttga gtctcgagga cggggcgatc ttgaacagca
     1621 cgcggcctgc caccgtgatt cctgcggggg cgccggtcaa gacggtgaag aaggggacga
     1681 aaccgacgtc gtagcaggaa gcacgcggag gtcttgggct aagggcggcc gggcgcaacg
     1741 cgtttgcgcg aggggaggca gccctagggg cgcgccgccc tccggcccga cattctcctc
     1801 gagtttgctc accacctcgg cgggcctccg ggagggcgcc ctccgcggct gcctcccctc
     1861 gcgtcaaaag acgacttcgc ccggcggtcg cttcgctccc gcctcccccg ctacctacac
     1921 tcttcggact cactccctgc attgtcggca atcacggggt gctctaccca cggattgtca
     1981 cgaccatcgt gggcattcat ggttctgctc cgccaacgga ctgaccggac acacccgatt
     2041 gtcacgtgac atgcgactga cggtcacaag acatgcgttg gattgtcccg agacaagtca
     2101 cgcccggaag agttcggtgg gggtcgggcg gaaccatgag gaagtacggg aggcgggagc
     2161 gaagcgacac gccgggccca accgtctttt gacgcgaggg gaggaacgcg gagggcgccc
     2221 tcccggaggc ccgccgaggt ggtgtgcaga gtcgaggaga atgtcgggcc ggagggcggc
     2281 gcgcccgcag cgttgcctcc cctcgcgcca aacgcgtagc gcccggccgc ccttagccca
     2341 gacctccgcg tgcgtcgtca gtggtgggta ccgagaaagc tccaccccag agcggacccc
     2401 accgcgatga ccacgtcctt ggggattctg ccgaaaagca cgacgatcag aaactgaggc
     2461 caggccacgc ccgcgatgcc cgacatgaag cagagcgcgt ccgccggtga gaacggaaag
     2521 aggaagatcg ggatcagcgc cagccatcgc tttccgccga tcagcttctg cgcctgctcg
     2581 agcgcgggct cgccgatcca ccgctccgcc actttccttc ctgccccgcg gccgatcgcg
     2641 aacgccgcgg tcgagccgat cgcggttccg atcgccgtga gcacggaacc ccaaagcgga
     2701 ccgtagagga cccccgcgat gagcgggacg aagacgcccg ggatcggcgc ggccaggatc
     2761 tgaccggcct gcaccccgag gagcgcgacc ggcccccacg ccccggcatg ccgcacccac
     2821 tgtttcagcg cctcgtgaga aatatgcggc cggagcagcc acccgagcac gccgatcacg
     2881 gcgccgatgc cgacgaagag aagcgtggcg cgaagcagag gatgcttggg catggggcgc
     2941 tcagccatgg cgggcgactc tacgggagcc gccggctcgg ggacatcgaa aagcgggcgc
     3001 cccggcggcc ccggtaccca aaaggcgcag cccttcactt tcgcgccatc cgatactatt
     3061 gtcggtagtt tcggacgccg ctcgccattt cgcgcgcgcc tgcgcgacag cctggaggaa
     3121 ccggatgacc gaccccttcg gagcgcgatc caccctggcg gtggatggac gctcctaccg
     3181 catcgcccgc ctggacgcgc tggagcgcgc cggcctctcg ctgcagcgcc tccccttctc
     3241 ccttagggtc ctgctggaga acctcctccg ccgcgtggac ggtaaggtgg tcacggcgga
     3301 ggacgtggag gcggtggcgc gctggaatcc ggcggcgg
//
LOCUS       DASSLZ010000026         5583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1278117, whole genome shotgun sequence.
ACCESSION   DASSLZ010000026 DASSLZ010000000
VERSION     DASSLZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278117"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VFM00_00905"
     CDS             complement(<1..1040)
                     /locus_tag="VFM00_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET7496834.1"
                     /translation="MRGGDFSVRLPGNWTGLEGKIADTFNDIAASNQRMAEELERVGQ
                     VVGKKGRTRERMRIARGDGAWGEMQVSVNTLIDDLVWPTREVTRAIAAVARGDLLQTV
                     GTEVDGRPLEGEFLRSANIVNTMIKQLAVFTSEVTRVAREVGSEGKLGGQAQVREVSG
                     VWKDLTESVNSMASNLTAQVRNIAEVTIAVADGDLSRKITVDVRGEILQLKEAINTMV
                     DQLRSFASEVTRVAREVGTDGKLGGQAVVPGVAGTWKDLTDSVNAMASNLTAQVRNIA
                     EVTTAVARGDLSRKITVDVKGEILELKNTINTMVDQLNAFAAEVTRVAREVGTEGKLG
                     GQAQVPGVGGTW"
     gene            complement(1304..5569)
                     /locus_tag="VFM00_00910"
     CDS             complement(1304..5569)
                     /locus_tag="VFM00_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HET7496835.1"
                     /translation="MRVVFDARHGASSNAWSDMVSSLTARGAVFTLNTRRLTAGVLAG
                     ADVYWISDDAGAFDADERAALAAWVRSGGSLLLEGASAAALPEFDALLDSTGVPLRYE
                     AGEGITGPTTRIYPFEITRDVVAADMPSSTARLHATGAGATVLIEDAQGFPTLGASFV
                     GRGRVLAAAGRLFADVSSIFADNRKVASQAFGWLGGAAWLSVTPGSGSTAAGQSTTVT
                     VAFDAARLAGADYRGALAVRTNDPDRSDFLVPVDLSVTPAPDAVATPASLDLGTVFVG
                     ASRLDSIRVRNVGVLPLRVSSAAMDHPDFTAVFDSLTLAPGASFTIPVRYAPASVGPA
                     SATLTVATDDPDQSEIRIGVRAAGAPPPDADVVAGPVGASLLTGGTATRTITVANRSG
                     SDLQYAIRFEEEAPPSATPAAGATVMASLPRTWAEARESALSFHGRVTPPATGTAGAL
                     AAAPDSLPLVVLDPQGDGSVEDLVALRAWARGGVLSIRIETATDIAPLNFGGYLSLDL
                     DQNRNTGREPSFGTPRQDIGAEFEVSLFSVGYGTVDLLDALTGNYVQSFPVQVGPRSV
                     SFAVPLSWLNGDDGRINATGVIGNFVGATDWFPDRGHGTIGGLWLAASPHEGTVAAGS
                     EASVALTLDALALPGGAYRGTIVVESNDPDEPRLEVPASLDVTDAPVAVATATAVHFG
                     TVFLGRTSEAEVAVGNEGRAPLTVSSVASDLSDFTAAPASFTVEPGAVQTIRLTFTPA
                     ADGPREGTLTIAHDAAGGPIAIAVTGSGRVPPVVQVAPASLQASVGLGERSTTSFSIR
                     NTGGSALDYGVEPLNGAPAVAVQAGLALARDEEDPRPGIAPPAALGLGGPDGFGYRWV
                     DSNQPGGPVYDWQDIAAIGTPIPIQNLDQNSGPLPIGFPFSFYGHEYTTFNVCTHGWI
                     SFTDTTVQFANQPLPTTAAPNNLLAVYWDDLNFGGVQRAVYYNDGTRLIVQFTNVPRR
                     QRGGPFTFQAILYPTGAIVYQYRLMGPPLDSGTIGIQNRTKTDGLTVVFNAAYLHDGM
                     AVRFSAAPSWLSVAQRFGSVDPGDSAGVAVALESRGLFAGDYHGTLRVQTNDPAHLET
                     LVPVLLHATGIPRLAFSPASFRFDTLDVGQSAADTLRLTNPGTDRLDVALAFDKPDYA
                     ADPASLSLAPLQSAPVLVRFAPTAGGDRSGALTLTTNDPETPSATVPLTGAGRLSARE
                     LAAEIVPAVIRRGSKGKEIRARIVFPPDLDAGAVDLAGIRLQGSVAPLPGSRLRDLDG
                     DGRPDLDLAFERGAVERALPNGNAVPVSITGQDGGIRFEARGTVRVLGSAVAAAGVEF
                     DEDLIPAANALRASAPNPFAAATTLRFDLADGGVAALRVYTAGGRLVRTVFQAPLPAG
                     RFRALWDGRDDRGVPMPSGVYFTRLEVSGPAPFRGVGRIVRIR"
BASE COUNT          789 a         1983 c         2036 g          775 t
ORIGIN      
        1 ttccaggtcc cgccgacgcc cggcacctgc gcctggccgc ccagcttccc ttcggttccc
       61 acttcgcgcg ccacgcgcgt cacctcggcg gcgaacgcgt tcagctggtc caccatggtg
      121 ttgatggtgt tcttgagctc gaggatctcc cccttcacgt ccaccgtgat cttgcgcgag
      181 aggtccccgc gcgccacggc cgtggtcacc tccgcgatgt tccgcacctg cgccgtgagg
      241 ttcgacgcca tggcgttcac ggagtcggtg agatccttcc aggttccggc cacgccgggc
      301 accaccgcct ggccgcccag cttgccgtcg gtccccacct cgcgcgccac gcgcgtcact
      361 tccgaggcga aggagcggag ctggtccacc atcgtgttga tggcctcctt cagctggagg
      421 atctcgcccc gcacgtcgac cgtgatcttg cgcgagaggt cgccgtcggc caccgcgatg
      481 gtgacctcgg cgatgttccg aacctgcgcc gtcaggttcg acgccatcga gttcacgctc
      541 tcggtgaggt ctttccagac gccgctcact tcgcgcacct gcgcctgccc gcccagcttc
      601 ccttcggagc cgacctcgcg cgccacgcgg gtcacctcgg acgtgaagac ggcgagctgc
      661 ttgatcatcg tgttcacgat gttcgccgag cggaggaact ccccttccag ggggcgcccg
      721 tccacctcgg tgccgacggt ctgcagcagg tcgccgcggg ccacggcggc gatcgcgcgg
      781 gtcacctccc gcgtgggcca gaccagatcg tcgatcaggg tgttcacgga cacctgcatc
      841 tcgccccagg cgccgtcgcc gcgggcgatg cgcatccgct cgcgcgtgcg ccccttcttc
      901 ccgacgacct gcccgacgcg ctccagctcc tcggccatac gctggttgga ggcggcgatg
      961 tcgttgaacg tatcggcgat cttgccctcg aggcccgtcc agttgccggg caggcggacg
     1021 gagaagtcgc cgccgcgcat cgcctggagc gcctggagga ccgaatcgag gggcggggct
     1081 tcggactcga gattggcttc cccgttgacc ggggtcgcca cggaggaatc actgcgcggt
     1141 gggttcatgt gcggactcct ggacggccat ctattcgcaa ggagcgacgt gcggcatagc
     1201 caggttgcct cgcccggcca tggtcctaaa gtctaccccg gagcctccgg gggtgcgcca
     1261 gcagattgtt ggaaacgggc gccggtatcg gccgtccgga cttctatcgg atccggacga
     1321 tccgccccac cccgcggaag ggtgccgggc cggagacctc gagccgcgtg aagtagacgc
     1381 cggagggcat cggcacgccg cgatcgtcgc ggccgtccca gagggcccgg aagcgtcctg
     1441 ccggcagcgg agcctggaac acggtccgga ccagcctgcc gccggcggtg tagacgcgga
     1501 gcgcggcgac tccgccatcg gccagatcga atcggagcgt cgtggcggcc gcgaaggggt
     1561 tgggggcgct ggcgcgcagg gcgttcgccg ccgggatcag gtcctcgtcg aactccacgc
     1621 ccgccgcggc gaccgccgac cccagcaccc ggaccgttcc cctggcctcg aagcggatcc
     1681 cgccgtcctg cccggtgatc gagacgggga ccgcgttccc gttcggcagc gcccgctcga
     1741 ccgcgccgcg ctcgaacgcg agatcgagat cgggacgccc gtccccgtcc aggtcccgca
     1801 ggcgggagcc tggcaacggt gccacggagc cctgcagccg gatcccggcc aggtccaccg
     1861 ccccggcatc gaggtccggc gggaacacga tccgcgcgcg gatctcctta cccttgctgc
     1921 cgcgccggat gacggcgggg acgatctccg ccgcaagctc gcgcgccgag agcctccccg
     1981 cgccggtcag cggaaccgtc gccgacggcg tctccgggtc gttggtggtc agcgtgagcg
     2041 cgccgctccg gtctcccccc gcggtcggag caaaccgcac gagcaccggc gcgctctgga
     2101 gcggggcgag cgaaagcgac gccgggtcgg ccgcgtagtc cggcttgtcg aacgcgagcg
     2161 cgacgtcgag ccggtcggtg cccgggttcg tcaggcgcag cgtgtcggcg gcggactggc
     2221 cgacgtccag cgtatcgaag cggaaggacg cggggctgaa cgcgaggcgc gggatcccgg
     2281 tggcgtgcag gagcaccggc accagcgtct cgagatgggc gggatcgttc gtctggacgc
     2341 gcagcgtgcc gtgatagtcg cccgcgaaga gcccgcgcga ttcgagcgcg acggcgacgc
     2401 ccgcgctgtc gccgggatcc acgctgccga agcgctgcgc cacgctcagc cacgagggag
     2461 cggcggagaa gcgcacggcc atgccgtcgt gcaggtaggc ggcgttgaac acgaccgtga
     2521 gaccgtcggt cttggtccgg ttctggatgc cgatcgtccc gctgtcgagc ggaggaccca
     2581 tcaggcggta ctggtacacg atggcgcccg tgggatagag gatcgcctgg aaggtgaacg
     2641 gtccgccccg ctggcgccgg ggcacgttcg tgaactgcac gatcagccgc gtgccgtcgt
     2701 tgtagtacac ggcgcgctgc acgccgccga agttcaggtc gtcccagtag accgccagca
     2761 ggttgttcgg cgcggcggtg gtgggaagcg gctggttcgc gaactggacg gtcgtgtcgg
     2821 tgaacgagat ccagccgtgc gtgcagacgt tgaaggtcgt gtactcgtgc ccatagaagg
     2881 aaaaggggaa tccgatcggg agcggccccg aattctgatc cagattctgg atcgggatcg
     2941 gcgttccgat cgccgcgatg tcctgccagt cgtagaccgg cccgcccggc tggttgctgt
     3001 ccacccaccg gtagccgaat ccgtccgggc ccccgagccc gagcgcggcc ggaggcgcga
     3061 tcccgggacg cgggtcctcc tcgtcgcggg cgagcgcgag cccggcctgg accgccaccg
     3121 ccggcgctcc attcaggggc tcgacgccgt agtcgagggc ggagcctccc gtgttccgga
     3181 tcgagaacga cgtcgtgctc cgttccccca gcccaaccga ggcctggagc gacgccggag
     3241 cgacctggac cacgggcggc acgcgcccgg agccggtcac ggcgatcgcg atcggccccc
     3301 cggcggcgtc gtgcgcgatc gtcagcgtcc cttcgcgcgg cccgtccgcc gcgggggtga
     3361 aggtgagccg gatggtctgc acggcgcccg gctcgaccgt gaacgacgcg ggggccgcgg
     3421 tgaagtcgga gagatcgctc gcgaccgagg agacggtgag cggcgccctc ccctcgttgc
     3481 ccaccgcgac ctccgcctcc gaggtgcggc cgagaaacac cgttccgaag tggaccgcgg
     3541 tcgcggtggc cacggccacc ggcgcgtcgg tcacgtcgag ggaggccggc acctcgagcc
     3601 gcggctcgtc gggatcgttg ctctcgacga cgatcgtgcc gcggtaggcg ccccccggca
     3661 gggccagcgc gtccagggtc agggcgacgc tggcctcgct ccccgccgcg accgttccct
     3721 cgtgcgggga ggcggcgagc cagagcccgc cgatcgtccc gtgaccacgg tcggggaacc
     3781 agtcggtggc gccgacgaaa ttcccgatga cgccggtggc gttgatgcgg ccgtcgtcgc
     3841 cgttcagcca cgagaggggg acggcgaagg agacggagcg gggtcccacc tggacgggga
     3901 agctctggac gtagtttccg gtcagggcgt cgagcagatc gaccgtgccg tagccgaccg
     3961 agaagaggct gacctcgaac tccgccccga tgtcctgccg cggcgttccg aagctgggct
     4021 cgcggccggt gttccggttc tggtccaggt ccagcgagag gtatcccccg aagttgagcg
     4081 gcgcgatgtc ggtcgcggtc tcgatccgga tgctcaggac gccgccgcgc gcccaggcgc
     4141 gcagcgcgac caggtcctcc accgatccgt cgccctgggg atcgaggacg acgagcggga
     4201 gcgaatcggg cgccgcggcg agcgctcccg cggtgccggt cgcgggcggc gtgacgcgcc
     4261 catggaacga gagcgcgctc tcgcgtgcct cggcccacgt cctcggaagc gaggccatga
     4321 cggttgcgcc ggcggccggc gtcgcgcttg ggggcgcttc ctcttcgaaa cggatcgcgt
     4381 actggaggtc gctcccggag cggttggcga ccgtgatcgt gcgcgtcgcc gtcccgccgg
     4441 tgagaagcga tgcgcccacc ggccccgcca cgacgtccgc gtcgggcggc ggcgctcccg
     4501 ccgcacggac cccgatccgg atctcggact gatcgggatc gtcggtggcc accgtcaggg
     4561 tggccgaggc cgggccgacc gaggcgggcg cgtagcgcac cgggatcgtg aacgaagcgc
     4621 ccggcgccag ggtgagcgag tcgaagacgg cggtgaaatc gggatggtcc atcgcggccg
     4681 aggacacgcg gagcggaagg acgccgacgt tccgcacgcg gatcgagtcg aggcgcgagg
     4741 caccgacgaa gaccgtgccg agatcgagcg acgcgggtgt cgccacggcg tcgggggccg
     4801 gcgtcaccga caggtccacg gggaccagga agtcgctccg gtcgggatcg ttcgtccgga
     4861 cggcgagcgc gccgcgatag tcggcgccgg ccagccgcgc ggcgtcgaag gcgaccgtca
     4921 ccgtcgtgga ctgccccgcg gccgtcgatc ccgaccccgg ggtgacggag agccacgccg
     4981 cgcctcccag ccagccgaac gcctgcgagg cgactttccg gttgtcggcg aaaatggagg
     5041 agacgtcggc gaagagccgc cccgcggcgg ccaggacgcg gccgcgcccg acgaacgacg
     5101 cgccgagcgt ggggaatccc tgcgcgtcct cgatcaacac cgtcgctccg gctcccgtcg
     5161 cgtggagccg cgccgtggag ctgggcatgt cggccgcgac gacgtccctc gtgatctcga
     5221 agggatagat cctcgtggtc gggccggtga tcccctcgcc ggcctcgtag cgcagcggga
     5281 ctcccgtcga gtcgagcagc gcgtcgaact cgggcagggc ggccgccgac gcgccctcga
     5341 gcaggaggga gccgcccgag cgcacccagg ccgcgagcgc ggcgcgctcg tcggcgtcga
     5401 acgcccccgc gtcatcggag atccagtaga cgtccgcccc cgccagcacg cccgccgtca
     5461 ggcgccgggt attcagcgtg aagaccgctc cgcgcgcggt gagcgagctg accatgtccg
     5521 accaagcgtt ggaggaggcg ccgtgccgcg cgtcgaagac gacgcgcacg tcggagatct
     5581 ccg
//
LOCUS       DASSLZ010000027         4570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1532547, whole genome shotgun sequence.
ACCESSION   DASSLZ010000027 DASSLZ010000000
VERSION     DASSLZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532547"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VFM00_00915"
     CDS             complement(<1..1136)
                     /locus_tag="VFM00_00915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7496836.1"
                     /translation="MPFEGPAAMIGKKLRAYEITEEIGSGGMATVYRAYQPSMDRHVA
                     IKVIRSSILHDKALRERFQREARLIARLEHPHLLPVYDFDGEHDPPYIVMRYLEGGTL
                     KQVHQKGAVPREEFLYVLRQLAGALDYAHRQGVVHRDLKPSNVMIDREGNAFLTDFGI
                     ARATGGDKDLTGTGLMIGTPGYMSPEQARADGVVDKAADIYSLGVIAFEILTGSPPYD
                     HENGFEVILAHINSPVPKASERARDLPKAVDPVLARALAKAPTERYATATELVEALTR
                     ALKIKPSDAPSEIRSMTQTISVDQLKARQGTAKSDTPSAATPSDQQRQMTAVSIDVKE
                     LAEILYEKGADAERVRSTMDALWSALSETAKGFGGTIHSRNEDGA"
     gene            1286..2242
                     /locus_tag="VFM00_00920"
     CDS             1286..2242
                     /locus_tag="VFM00_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HET7496837.1"
                     /translation="MLNRWILACAVGLSLAAAPACGPYAGAPGAGPVRPIPRIARVLL
                     VSVDGLRPDLALRADAPAIRGLMKRGSFSLWAETTDLAVTLPSHVSMLTGVPPSQHGI
                     VWNSALPEKRGVYPAWPTLFEIARRAGYTTAMAAGKSKFSTLAKPGTLTWSFVPESSI
                     TPDAAVTDRAIAWIERSAPQVLFVHLPEVDSAGHGYGWGSPEQLAAIAGADRCIGRLL
                     DALEKRGVMDSTLVLVTADHGGAGKNHGPDDPRSRMIPWIAAGPDVRADYDLTGEDHL
                     TVRTEDTFATLAYVLGIASPQPIVGRPVLSIFSMTPERETRH"
     gene            2363..>4570
                     /locus_tag="VFM00_00925"
     CDS             2363..>4570
                     /locus_tag="VFM00_00925"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7496838.1"
                     /translation="MVPARVHAAFRRCVLSLLGLFLFAAMPAIAAAKTNQPPHVKLNI
                     VKYTLPNGLDVILCENHRLPTVGVNVWYHVGPANEEPGRTGFAHLFEHMMFKGSRHVP
                     DGKHWTYLQTAGATLVNGTTDFDRTNYLEDLPSNQLEVALWLESDRMGFLIDGVDAIK
                     LANQQDVVRNERRQSTENAEYGIVEEELFHQLYPKDHPYYADVIGSHQDIQAAKLDDV
                     RDFFKRYYVPNNASIAIVGDIDVAKTKALVAKYFGSIPRGGDVPPIRAVTPPITAERR
                     ATVTDNVSLPRVYMGWITPKAFAADEANGAIASDILSGNVNPYGDGNGKASRLYQKLV
                     YEMRIAQDVKTIHNPLQLGSIFEVIATAKPGHTAEELEKAIDAEIAKMAAEGPTLAEV
                     DASRQAVYLGEVSSLERVGFFNGIADRLNRYNYYVKTPDYLDKDLGRYAEVTPQSVKR
                     FVAQYLTKDHRAVVYGIPGEKQLGAAVPTPPAPTSPGVPPPGEDKEAWRSQAPEPAQA
                     SVLRLPRPTRFTLANGLTVYHLEDHNLPLVTASLVFRSGSAADPADLPGLAYFTADML
                     EQGTKSSDAMGIAGRVHALGTNWNVSFDMDGSSRAVQSLSPSASSAMALLAEVALQPT
                     FPQAEIDRVRQDRLAALLQQKDSPPETATRVFLSSLYGPNHPYGHTILGTEAAIQKIG
                     RDDLVRFHETQHTPGNAALVVVGDIRESDVRKMAQDLFGSWKGATPAPLALPAG"
BASE COUNT          733 a         1604 c         1513 g          720 t
ORIGIN      
        1 gccccgtctt cgttccggct gtggatcgtg ccgccgaatc ccttcgcggt ctcgctcagc
       61 gccgaccaga gggcgtccat cgtcgagcgg acccgctcgg cgtccgcgcc cttctcgtag
      121 aggatctccg ccagctcctt cacgtcgatc gagaccgcgg tcatctgccg ctgctggtcg
      181 gacggagtcg ccgcgctcgg cgtgtccgac ttggccgtcc cctgccgcgc cttgagctgg
      241 tccacggaga tcgtctgcgt catcgagcgg atctccgagg gggcgtcgct gggcttgatc
      301 ttgagggcgc gcgtcagggc ctcgaccagc tcggtcgcgg tggcgtagcg ctcggtaggc
      361 gccttggcca gggcccgcgc gagcaccggg tccacggcct tgggcaggtc gcgggcccgc
      421 tcgctcgcct tgggaaccgg gctgttgatg tgcgccagga tcacctcgaa cccgttctcg
      481 tgatcgtacg gaggcgagcc ggtcagaatc tcgaacgcga tgacgccgag ggaatagatg
      541 tcggcggcct tgtccacgac gccgtcggcg cgagcttgtt ccggcgacat gtagccaggc
      601 gtgccgatca tgagcccggt gcccgtcagg tccttgtcgc cgcccgtcgc gcgcgcgatg
      661 ccgaagtcgg tcaggaaggc gttcccctcg cgatcgatca tgacgttgga gggtttgagg
      721 tcccgatgga ccacgccctg ccggtgcgcg tagtcgagcg cccccgccag ctggcgcagc
      781 acgtagagaa attcctcgcg cgggacggcc cccttctgat ggacctgttt cagggtgccc
      841 ccctcgagat accgcatcac gatgtagggg ggatcgtgct cgccgtcgaa gtcgtacacc
      901 ggaaggaggt gcgggtgctc gagccgcgcg atgagccggg cctcgcgctg gaagcgctcc
      961 cggagcgcct tgtcgtggag gatgctggac cggatcacct tgatcgccac gtgccggtcc
     1021 atgctcggct ggtaggcccg atagaccgtg gccatcccgc ccgagccgat ttcctcggtg
     1081 atttcgtacg cgcgcagctt cttgccgatc atcgcggcag gtccctcaaa tggcatcggt
     1141 cacatggata gggggatgca gcccagaagg ataccgcaaa gaaacgggtt cggacagaac
     1201 gcgcgtgata caaatgaccc cagtgtcacc ggatcgaaat ggtttcgtcg ccgcggtgct
     1261 ccggaggtcc gaccgaggtg cttggatgct gaatcgatgg atcttggcct gtgccgtcgg
     1321 cctctccctg gcggccgctc ccgcgtgcgg cccctacgcg ggggcacccg gggccggccc
     1381 ggtccgcccg attccccgga tcgcgcgggt gctcctggtg agcgtcgatg gtctccgccc
     1441 ggatctggcc ctccgggccg acgctcccgc gatccgcggt ctcatgaagc gcgggagctt
     1501 ctcgctttgg gcagagacga ccgacctcgc cgtgacgctt ccctcgcacg tctccatgct
     1561 gaccggcgtg ccgccctccc agcacgggat cgtctggaac agcgccctgc ccgaaaagcg
     1621 cggggtctac cccgcgtggc cgacgctgtt cgaaatcgcg cgacgcgccg gctacacgac
     1681 cgcgatggcc gccggcaaat ccaagttctc gaccctggcc aagccgggga cgctgacctg
     1741 gagcttcgtg ccggaatcgt cgatcactcc ggacgcggcc gtcaccgacc gcgcgatcgc
     1801 atggattgag cgctccgcgc ctcaggttct cttcgtgcat ctgcccgagg tggactccgc
     1861 ggggcacggc tacggctggg ggtcgccgga acagctcgcc gccatcgcgg gcgcggaccg
     1921 gtgcatcggc cgcctgctgg atgccctgga gaagcgcggc gtcatggatt ccacgctcgt
     1981 cctggtcacc gcggatcatg gcggggcggg caagaatcac ggacccgacg atccgcgaag
     2041 ccggatgatt ccgtggatcg cggccggtcc ggacgtgcgc gcggactacg atctcaccgg
     2101 cgaggaccac ctgacggtgc gcaccgagga caccttcgcg acgctcgcct acgtcctcgg
     2161 catcgcctcg ccgcaaccga tcgtcgggcg ccccgtgctg tcgatcttct ccatgacgcc
     2221 ggagcgcgaa acgcggcact gaggccgcat ccgccgcata gggcctctct tacccggaat
     2281 gactttctcc gcgcaggagg aacgcgagcg cgtagaatgc tcggcacgca cgtcgcgccc
     2341 acttagccac aggagaggaa tcatggtgcc cgcccgcgtc cacgccgcct tccgccgttg
     2401 tgtcctttcg ctcctcggcc tgttcctctt cgcggccatg cccgcgatcg cggccgccaa
     2461 gacgaatcag cccccgcacg tgaagctgaa catcgtgaag tacaccctcc ccaacgggct
     2521 ggatgtgatc ctctgcgaga accatcggct ccccacggtc ggggtgaacg tctggtatca
     2581 cgtcggcccc gcgaacgagg agccggggcg cacggggttc gcgcacctgt tcgagcacat
     2641 gatgttcaag ggctcgcgcc acgttcccga cgggaagcac tggacctacc tccagaccgc
     2701 gggcgcgacg ctggtgaatg gcacgaccga cttcgaccgc acgaactatc tcgaggacct
     2761 gccgtcgaac cagctcgagg tggcgctctg gctggagagc gaccggatgg gcttcctgat
     2821 cgacggcgtg gacgcgatca agctggcgaa ccagcaggac gtggtgcgga acgagcggcg
     2881 gcagagcacg gagaacgcgg agtacgggat cgtggaggag gagctgttcc accagctcta
     2941 tcccaaggac cacccctact acgcggacgt gatcggatcg caccaggaca tccaggccgc
     3001 gaagctggac gacgtgcgcg acttcttcaa gcgctactac gtccccaaca acgcgagcat
     3061 cgccatcgtg ggcgacatcg acgtcgccaa gacgaaggcg ctggtcgcga agtacttcgg
     3121 ctccattccg cgcggcggcg acgtgccgcc gatccgggcc gtgacgccgc cgatcaccgc
     3181 ggagcgccgc gccaccgtga ccgacaacgt ctccctgccg cgcgtctaca tggggtggat
     3241 cacccccaag gcgttcgcgg ccgacgaggc gaacggcgcg atcgcctccg acatcctctc
     3301 ggggaacgtg aacccctacg gcgacgggaa cggcaaggcg agccgtctgt accagaagct
     3361 ggtctacgag atgcggatcg cccaggacgt gaagacgatt cacaacccgc tccagctggg
     3421 gtcgatcttc gaggtcatcg cgaccgcgaa acccgggcac accgcggagg agttggagaa
     3481 ggcgatcgac gcggagatcg ccaagatggc tgctgagggc ccgaccctgg cggaggtgga
     3541 cgcctcccgc caggcggtct atctcggcga ggtctccagc ctggagcgcg tcgggttctt
     3601 caacggcatc gccgaccgcc tgaaccgcta caactactac gtgaagaccc ccgattacct
     3661 ggacaaggac ctcgggcgct acgccgaggt gaccccgcag tcggtgaagc gcttcgtagc
     3721 ccagtacctg acgaaggacc accgcgccgt ggtctacggg attccgggcg agaagcagct
     3781 gggcgccgcc gtgcccacgc ccccggcgcc gaccagcccc ggcgttccgc ctccgggcga
     3841 ggacaaggag gcctggcgca gccaggcgcc cgagcccgcg caggcctcgg tgctccggct
     3901 gccgcgtccg acgcgcttca cgctggccaa tggcctcacg gtctaccacc tcgaggacca
     3961 caacctgccg ctcgtgaccg cgtcgctcgt cttccgctcg ggcagcgccg ccgatccggc
     4021 cgatctgccg gggctcgcct acttcacggc cgacatgctc gaacagggca cgaagtcctc
     4081 ggacgcgatg ggaatcgccg gccgtgtcca tgcactcggc acgaactgga acgtgagctt
     4141 cgacatggac ggcagctcgc gcgcggtgca aagcctctcc cccagcgcct cgtccgcgat
     4201 ggcgctcctc gccgaggtgg cgctccagcc cacgttcccc caggcggaga tcgaccgcgt
     4261 gcggcaggac cgcctggcgg cgctgctcca gcagaaggat tcgccgccgg agacggccac
     4321 ccgggtcttc ctctcgtcgc tctacgggcc caaccatccg tacggccaca ccatcctcgg
     4381 gaccgaggcc gcgatccaga aaatcggacg tgacgatctc gtgcgcttcc acgagacgca
     4441 gcacacgccg gggaacgcgg ctctggtcgt ggtaggcgac atccgggaat ccgacgtccg
     4501 gaagatggcc caggacctgt ttggctcctg gaagggtgcc acccccgcac cgctggcgct
     4561 accggccggg
//
LOCUS       DASSLZ010000028         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_643744, whole genome shotgun sequence.
ACCESSION   DASSLZ010000028 DASSLZ010000000
VERSION     DASSLZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643744"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1828)
                     /locus_tag="VFM00_00930"
     CDS             complement(<1..1828)
                     /locus_tag="VFM00_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7496839.1"
                     /translation="MLFALLASPFAGASAAPAPAPDARAKAARADAIERSSRFTSPAP
                     RMAPVLSASALARIAVEGRPALWVVFTDKGIFDERSFGAAVRDAGARLTERARARRAK
                     EQGGRYAPDFDDVPVPGRYVDAVAATGARVRHVSKWMNAVSVMADEGEARRLAALPFV
                     RAILPLQYSRPVAPVSVGPTIEGAPGNDAHGSAPRPEGAAPAPQGERAATILSKPVNQ
                     SGYGLSFTQLNGIFVPAVHDSGYSGAGVILAMFDTGFNKSHSATIQLKRIAEWDFVWE
                     DSETANQIPPDASGQWDHGTGTWSVAGGYWINNLIGPAYNATFALAKTEYVPSETHAE
                     EDNWVAAAEWADSLGADVISSSLAYLTFDSGVDYTWADLNGHTTVVAQGAILAHRRGI
                     VVANAMGNSGPGGRTLDSPADVDSILSCGAVDNSNNIATFSSRGNTSDGRIKPEVVAQ
                     GVSTTWAVASNNNATTLASGTSLSTPLVGGSAALVREAHPEWTVEQVRQAFMTTADRA
                     GTPDSIYGWGRINVRAAIWSSAYGPPVFPRPFDLLVPSKNSVVNATPLTLRWRGTTDP
                     QSEAISYTVTLRSLASGAVVFTKTTTDSSAVVTGYLGPSASYE"
     gene            complement(1947..>2567)
                     /gene="fusA"
                     /locus_tag="VFM00_00935"
     CDS             complement(1947..>2567)
                     /gene="fusA"
                     /locus_tag="VFM00_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872259.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7496840.1"
                     /translation="RYKKQTGGRGQFGDVHVRFEPLKPGSGFEFVDEIVGGSVPRNFI
                     PAVEKGIVEAMHEGVLAGYPVVDFRAALYDGSYHTVDSSEMAFKIAGALAFREGVRDA
                     GPVLLEPIVEITVRVPKDFVGAVTGDLSSKRGKILGMDSETRYDQIRAHVPQAELYKY
                     STHLRSLTQGRASFHTKFSHYEEIPRELADKVIAQAKADREAMASA"
BASE COUNT          374 a          876 c          928 g          389 t
ORIGIN      
        1 attcgtagga cgcgctggga cccaggtagc cggtcacgac cgcggagctg tcggtggtgg
       61 tcttggtgaa gacgacggcg cccgaagcga gcgagcggag cgtgacggtg tagctgatcg
      121 cctcgctctg cgggtcggtc gtgccccgcc agcgcagcgt gagcggcgtc gcgttcacga
      181 ccgagttctt ggacggcacg aggagatcga aggggcgtgg gaagaccggc gggccgtagg
      241 ccgagctcca gatggcggcg cgcacgttga tccgccccca cccgtagatg ctgtcggggg
      301 tgccggcgcg atcggcggtg gtcatgaacg cctgccgcac ctgctcgacg gtccactccg
      361 gatgcgcctc gcgcacgagc gcggcagacc cgccgacgag gggcgtggag agcgaggtcc
      421 cgctcgcgag cgtggtggcg ttgttgttgc tcgccacggc ccaggtggtg ctgacccctt
      481 gcgcgaccac ctccggcttg atgcgcccgt cggacgtgtt cccgcggctg gaaaaggtgg
      541 cgatgttgtt ggaattgtcc accgcgccgc acgacaggat gctgtcgacg tcggccgggc
      601 tgtcgagcgt gcggccgccg ggcccggagt tgcccatggc gttggccacc acgatcccgc
      661 ggcggtgcgc caggatggcg ccctgcgcga cgacggtggt gtggccgttc agatcggccc
      721 aggtgtaatc cactcctgag tcgaaggtga ggtaggcgag cgaggaagag atcacgtcgg
      781 cgcccaggga gtcggcccac tcggccgcgg cgacccagtt gtcctcctcg gcgtgcgtct
      841 ccgagggaac gtactccgtc ttcgcgagcg cgaacgtcgc gttgtacgcg gggccgatca
      901 ggttgttgat ccagtagccg cccgcgaccg accaggtgcc ggtgccgtgg tcccactgac
      961 ccgaagcgtc ggggggaatc tggttggcgg tctcgctgtc ctcccagacg aaatcccact
     1021 ccgcgatccg cttgagctgg atcgtggcgg agtggctctt gttgaacccg gtatcgaaca
     1081 tcgcgaggat gacgccggcg ccgctgtagc ccgagtcgtg gaccgccggg acgaagatgc
     1141 cattgagctg cgtgaacgac aggccgtacc cggactggtt cacaggcttg ctcaggatcg
     1201 tcgcggcccg ttctccttgc ggagccggcg ccgcgccttc cggccgcggc gctgaaccgt
     1261 gcgcatcgtt tcccggcgcg ccttcgatcg tggggcccac cgaaaccggc gccacggggc
     1321 gcgagtactg gagcggcagg atcgcgcgca cgaacgggag cgccgcgagc cggcgcgcct
     1381 ccccctcgtc cgccatcacg ctcaccgcgt tcatccactt ggagacgtgc cgcacgcggg
     1441 cccccgtggc ggcaaccgcg tcgacgtagc ggcccggcac cggaacgtcg tcgaaatcgg
     1501 gcgcgtaccg cccgccctgc tccttggcgc ggcgggcccg ggcgcgctcg gtgagccgcg
     1561 cgccggcgtc gcgcacggcc gcgccgaacg agcgctcgtc gaagatcccc ttgtcggtga
     1621 acacgaccca gagcgcgggg cgcccctcga cggcgatccg tgcgagcgcc gaggcgctca
     1681 gcaccggcgc catgcgtggc gcgggggacg tgaagcggga gctccgctcg atggcgtccg
     1741 cgcgtgccgc cttggcccgg gcatcgggcg cgggagcggg ggcggccgaa gcgccggcga
     1801 acggcgaggc cagaagggcg aaaagcacga ggccgccgag ggcggcctcg aacggcctgg
     1861 aacacgggga aacccgggct aacgtgacgc ggagccggcg gtgcgaggca aggcgaagat
     1921 ccattcgtgt cgtgctcccc ggccggctag gcggatgcca tcgcttcccg gtccgccttg
     1981 gcttgcgcga tcaccttgtc ggcgagctcg cgcgggatct cctcgtagtg gctgaacttg
     2041 gtgtggaacg aagcgcgccc ctgcgtgagg gagcgcaggt gcgtcgagta cttgtacagc
     2101 tcggcctgcg gcacgtgcgc ccggatctgg tcgtagcgcg tctccgagtc catgccgagg
     2161 atctttcctc tcttggatga cagatccccc gtgacggctc cgacgaaatc cttgggaacc
     2221 cgcaccgtga tctccacgat cggctcgagc agcaccgggc cggcgtcccg caccccctca
     2281 cggaaggcca gcgcgcccgc gatcttgaag gccatttccg acgagtccac cgtgtggtag
     2341 gagccgtcgt acaacgcggc gcggaagtcc acgaccgggt agccggccag caccccctcg
     2401 tgcatcgcct cgacgatccc cttctccacg gcggggatga agttgcgcgg aacggagccg
     2461 cccacgatct cgtccacgaa ctcgaacccg ctcccgggct tcagcggctc gaagcgcaca
     2521 tggacgtcgc cgaactggcc gcgcccgccc gtctgcttct tgtagcg
//
LOCUS       DASSLZ010000029         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1785420, whole genome shotgun sequence.
ACCESSION   DASSLZ010000029 DASSLZ010000000
VERSION     DASSLZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785420"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VFM00_00940"
     CDS             <1..1142
                     /locus_tag="VFM00_00940"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET7496841.1"
                     /translation="ATIVKLPVHMDDGTFRVFTGYRVQHSIIRGPAKGGIRYHPDVTL
                     EEVMALAAWMTWKCAVVGIPFGGGKGGIQCDPSKMSMGELERLTRRYTADMIDVIGPE
                     SDVPAPDVNTGEQTMAWIMDTYSMHARHTVTSVVTGKPLALGGSAGRREATGRGVLFC
                     VREAAKRIGLSLKGAKVVVQGFGNVGSVAADLLAKDGAKIVAVSDVHGGVQNPNGLDM
                     AALFKHLGEKKTVAGFSGGKPVQGKAILEVPCDILIPAALENQITHANAGKVKARIIA
                     EGANGPTTTRADKILNKAGVLVVPDILANSGGVTVSYFEWVQDRQGLFWREQEVNDRL
                     EHIMVQSFHDVAAMADEHKVSFRVAAFMLAIKRVVDAIQLRGVYA"
     gene            complement(1411..2334)
                     /locus_tag="VFM00_00945"
     CDS             complement(1411..2334)
                     /locus_tag="VFM00_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET7496842.1"
                     /translation="MKAIAPPRLRPGDRIGVVAPSGPVLPRALVSGARVLEEAGFRIV
                     FGKHVRDRRGHLAGSDRARLDDLNRMFRDPDIRCVLMARGGYGAMRLASGVDWGALRR
                     DPKLFAGFSDATFLHLGIARHAGVRTLHGPNLHGLGARRGTEVARLLAWLTQPMPSER
                     VRTFAVPRRVDGASGTARGCVMGGNLALVHYAAAAGHLPVLRGCILFLEEVNEPPYKV
                     DGMLCALTEGKWLKGVRGIVLGDFTRCLPRKGYRELRLRQVLTDHLKPLEIPAWSGLC
                     AGHGSRNYPVPFGARAVLAKGRLIYEEGVVS"
     gene            complement(2331..>2698)
                     /locus_tag="VFM00_00950"
     CDS             complement(2331..>2698)
                     /locus_tag="VFM00_00950"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET7496843.1"
                     /translation="ATARALAAWLVAMDRDSTLAAPFREGLAGPGENGTLKRRFPGLD
                     PGANLRAKTGTLTNVSSLSGYVTSASGERIAFAVITNGNRGSTALGKQLEERLVGMLA
                     RRGGEASGMTGSPPPRIPR"
BASE COUNT          455 a          932 c          914 g          397 t
ORIGIN      
        1 gcgccaccat cgtgaagctc ccggtccaca tggacgacgg gacgttccgc gtcttcaccg
       61 ggtaccgggt gcagcattcg atcatccgcg gcccggccaa gggcgggatc cgctaccacc
      121 ccgacgtcac gctggaagag gtcatggcgc tcgccgcatg gatgacctgg aagtgcgcgg
      181 tcgtcggcat tcccttcggc ggcggcaagg gggggatcca gtgcgatccc tcgaagatgt
      241 ccatgggcga gctggagcgt ctcacgcggc gctacaccgc cgacatgatc gacgtgatcg
      301 ggcccgagtc ggacgttccc gcccccgacg tgaacaccgg cgaacagacc atggcctgga
      361 tcatggacac gtactcgatg cacgcccgcc acaccgtgac gtcggtggtc accggaaagc
      421 cgctcgcgct gggcggctcg gccgggcggc gcgaggcgac cgggcgcggg gtgctcttct
      481 gcgtccgcga ggcggcgaag cggatcgggc tttccttgaa gggcgccaaa gtcgtcgtgc
      541 aggggttcgg caacgtcggc tcggtggcgg cggacctgct ggcgaaggat ggcgcgaaga
      601 tcgtcgcggt cagcgacgtg cacggcgggg tgcagaatcc gaacgggctc gacatggccg
      661 ccctcttcaa gcatctcggc gagaagaaga ccgtggccgg gttctccggc ggaaagccgg
      721 tccagggaaa agcgatcctc gaggtcccct gcgacatcct gattcccgcg gcgctggaga
      781 atcagatcac ccacgccaac gcgggcaagg tcaaggcgcg gatcatcgcc gagggagcga
      841 acggccccac gacgacgcgc gccgacaaga tcctgaacaa ggcgggcgtc ctggtcgtgc
      901 ccgacatcct cgccaacagc ggcggcgtga ccgtctccta cttcgaatgg gtccaggacc
      961 ggcagggcct cttctggcga gagcaggaag tgaacgatcg cctcgagcac atcatggtgc
     1021 agtccttcca cgatgtcgcg gccatggcgg acgagcacaa ggtgtcgttc cgggtagcgg
     1081 cgttcatgct ggcgatcaag cgggtggtgg acgcgattca gttgcgcggg gtgtatgcct
     1141 aacccgcgtc gcttgcgacg cggaaagcag catgcgcgct aaaaggcgcc ccttcatgag
     1201 gtgacgatgc cgttacgagg cggctctttc gcgcggcgaa gcagtgtccc acgcgaatga
     1261 cgtctgtcgc cgatttcacc gccgctggcg cgtgtgaaac tcgcgacact tgtcgctcaa
     1321 attggcccgg cctcgagtga gttcaacgag atgattcgag ctgctcaacg agatgaggtg
     1381 agcagttctg cggaatgaca tgaagcggtc ctacgaaacg acaccctcct cgtagataag
     1441 tctccccttc gcgagaactg ccctcgcgcc aaacggcacg ggatagttcc tgctcccatg
     1501 ccccgcgcat agccccgacc acgcggggat ctccagcggc ttcaggtgat cggtcagcac
     1561 ctggcggagg cgcagctcgc ggtacccctt tcgcgggagg cagcgggtga agtcgccgag
     1621 gacgatgccg cgcactccct tcagccactt cccctcggtc agcgcgcaga gcatcccgtc
     1681 caccttgtac ggcggctcgt tcacttcctc caagaacaga atgcatccgc gcagcacggg
     1741 caggtgaccc gcggccgccg cgtagtggac gagcgccagg tttcctccca tcacgcaacc
     1801 ccgcgcggtg cctgaagcgc cgtccacgcg gcgtggcacc gcgaacgtgc gcacccgctc
     1861 cgagggcatc ggctgggtca gccaggcgag aaggcgcgcg acctcggttc cccgccgcgc
     1921 gcccagtcca tgcaagttcg gaccgtgcag cgtgcgcacg ccggcgtggc gcgcgatccc
     1981 caggtgcagg aaggtagcgt cggagaatcc ggcgaagagc ttgggatcgc ggcgcagcgc
     2041 gccccagtcg acgccggagg cgagccgcat ggcgccgtaa ccgccgcgcg ccatgaggac
     2101 gcatcggatg tcggggtcgc ggaacattcg gttcaggtcg tccagccgcg cccgatcgga
     2161 gccggccaag tggccgcggc ggtcgcgcac gtgcttgccg aacacgatcc ggaagcccgc
     2221 ctcctcgagg acacgcgctc cggagacgag cgcgcgcggc agcaccggcc ccgagggcgc
     2281 gacgacgccg atccggtccc ccggacggag cctcggcggg gcgatcgcct tcaacgcgga
     2341 atgcggggcg gcggcgagcc cgtcatcccg ctcgcttccc ctccccgtcg ggccagcatt
     2401 cccaccaagc gctcctccag ctgcttcccc aatgccgtgc ttccccgatt gccgttcgtg
     2461 atcacggcga acgcgatccg ctcgcccgac gcggaggtca cgtaccccga aaggctgctc
     2521 acgttggtca gcgtccccgt cttcgcgcgc aggttggccc ccggatcgag gcccggaaac
     2581 cgccgcttca gggtgccgtt ctccccgggg ccggccagcc cctcgcgaaa cggagccgcc
     2641 agcgtcgagt cccggtccat cgcgacgagc cacgccgcca gggcgcgcgc ggtggcga
//
LOCUS       DASSLZ010000030        25872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_518684, whole genome shotgun sequence.
ACCESSION   DASSLZ010000030 DASSLZ010000000
VERSION     DASSLZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 25872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25872
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518684"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(475..549)
                     /locus_tag="VFM00_00955"
     tRNA            complement(475..549)
                     /locus_tag="VFM00_00955"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Asn,seq:gtt)
     gene            complement(630..9209)
                     /locus_tag="VFM00_00960"
     CDS             complement(630..9209)
                     /locus_tag="VFM00_00960"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HET7496844.1"
                     /translation="MGTIWVFGKWRDPSRRAPGSDREPLRGELLSVEHLEERARVLAA
                     SYTVARNPRRRARRLLPRLHENARVLRHAYRELANDVRRGEPVVPAAEWLLDNFHLVE
                     TEAVEVRKNLPHRYYLELPKLASRDLAGTARVHAMALEFIRHSDARFDLHRLTQFIGA
                     YQAVAPLTLGELWAWPSMLKLCLIENIRRLAGEIMESREGEADADAYFTRFETIRPTD
                     PIPPLPGNLSNGFVVQLVQRMRELGPRVGELRVELDRRLHEAGLTFDEALRSEHQRQT
                     MGQASMGNSITALRLVATTDWNRTIEQVSLMEQILRRDPAGVYGRMDFPSRDRYRQSV
                     EELSEPSGEAQVRVALRAVESARQAGERGEGEPATHVGYHLVGGGRRDFEVDVAYTPR
                     PAQRIRRLLFAECTLFYLGSIALVTALGVGLAVAWSRAWGAPVAMLPWIGLLTLIPAS
                     HVAVLFMQRMVHRLAHPRRLPRLDLQHGVPDEARTIVVVPVLLGSVAAARAHVEHLEV
                     QALGNEDRHIHFALLTDFRDATEPERPEDAAVLAAVTSGIEALNARYGNGARDRFYLF
                     HRPRRWNEREGVWMGWERKRGKIEEFNALLRGHPSSFQAVVGDPSILTSVRYVITLDA
                     DTRLPRETARKLIGIIQHPLNRPRFDPVLRRVTRGYGILQPRVSVTMASAAGSLFARV
                     YAGHTGVDIYTTAVSDTYQDLFSEGIFTGKGLYDVDAFMAALEGRVPENALLSHDLFE
                     GIYARTGLVSDIEVVDDFPSSVLAHAGRQRRWVRGDWQILAWLFPWVPTRGSLERNRL
                     PLIARWKILDNLRRSVLGPATLALLASAWTWLPGPPWVWTVAVILGMCFPLYSKIVRM
                     VKGPEPQQPVPVFLRDLGEEFQTAGAQVFLDITLLAYQSYEMLHAIAVTLVRLFFTRR
                     KMLEWETAATAAARSAGLSGKDQLVAFANGMWAGPAVAVLILVATVAFRIEALGAALP
                     LLSLWLVSPLIAYWLSLPARSRVVALEPHDRVELRRIARNTWRYFDTFAGEEDHWLPP
                     DNVQETPDRKVAHRTSPTNIGMALLATLAARDLGFIGAPEVLDRVERILNTLDGLEKH
                     EGHLLNWYDTLSLAPLQPRYVSTVDSANLAGSLMALAAGLRRLEPAPAEGFRHAAAVA
                     DTLALLRQSLDRILPEASAESIRERLRPALREVEAYEVRRFAARPDSPRLDGAGRALA
                     QALEVADLDRLEGPEAEEAAFWGETLVRLLRHPKAGDLPESWRERRAALAARAEALAD
                     EMDFRFLYDRDRRIFSIGYRLADMEGPGRLDVSYYDLLASEARLASFVAIAKGDVPPE
                     HWFQLGRALVGIAGVPTLVSWSGSMFEYLMPLLLMRNYPDTLLQRSSDSAVRAQIQYG
                     RMRGVPWGISESAFNEVDRHGNYQYKAFGVPELGLKRGLSEELVIAPYATALAAMVDP
                     VEAAANLRRLTREGAEGAFGFYEAIDYTPREAEEGDGKPVARADGHPKPAVVQAYFAH
                     HQGMSVVALSNAVLHDPMVSRFHSEARVQATELLLQERVPRFVPVTRPRPVEMTHLAV
                     PVLPMLPRRFRTPHTRYPHTAFLSNGRYVATVSNSGGGSSSCRGITVTRRREDSTLDP
                     ASQCLYLRDVRSGAVWSAAYQPIRREPDEYRATFLPDRVVIEQRLEAIQSKLEIAVSA
                     EDDVEVRRLSLTNRSPYLREIEVTSYDEVVLAPQADDLAHPVFGKLFLETECRPETAS
                     LLCSRRPRSSDDPRAWAVHVLSMEGRMQSAVEWETDRVRFLGRGRGTDDPIALDGRAL
                     SGTVGATLDPILSLRQRIRLAPGAFARLSFATGVASDRDAAVALCMKYADPNSAARTF
                     ALAATHLSISLRHLGIRVEEAQLYERLASRVFGVDRSLGPAADILARNVLGQPGLWGH
                     GISGDNPILLVRVLEPDDIGLVSQVLRAQDYWRLKGLVADLLILNDHPVSYRNEIHEQ
                     LNGLLDSGPWAAWKDKPGGAFLLTGDGMGEADRTLLAAVARAVLRGDAGSLEDQLNRA
                     YPDPVWPPALAIEGGGAASGGEEVSEDIEVPELLLWNGRGGFTLDGQEYAVVLNGADE
                     TPLPWANVLANPRFGSVVTAAGLSYTWSENSRENRLTPFANDPATEATGEAIYLRDDE
                     SGAAWGATPGPMRRRDDAGRWLVRHGVGVTRYVHRERGIRHELAVFVHRSEPVRFALL
                     TLANQTKRARRVSVFGYQEWRLGPPREREQVHVRTEFDPATATVLAENPYNQDFRGRV
                     AFAHAGVPVRSASGDRTEFLGRNGSVARPAALEREELSNAFGAGLDACAALQVSLDLE
                     PGETKQVILLLGEGADKDEALAMARRFGSVPAALSALQEVEEFWDGMLGAVQVRTPDD
                     SFDLIMNRWLLYESVSSRIWARTAFYQPGGAYGFRDQLQDVMALPFADPALFREHVLR
                     AAGRQFVEGDVQHWWHPNSGVGVRTRCSDDRLWLPFVVLRYLKSVGDASVLEERIPFL
                     EAPPLGPEEHEAYGRPQTSAQDGTLYEHCVRAIECSLTSGPHGLPLIGDGDWNDGMNR
                     VGRLGHGESVWLGWFLAMILREFAALAETRGDPDRAARYRGEAGRIASMMEQAWDGEW
                     YRRAYFDDGTPLGSAQNDECRIDSICQSWALLSGVAPPARAERAMDSVRSHLIRRDAS
                     VVMLLTPPFDASRLDPGYIKGYIPGVRENGGQYTHAAVWVVMAVAAMGSGDEAVELFH
                     LLNPINHTRSEGDVARYKVEPYVVAADVYTHPAHVGRGGWTWYTGSASWMYRAGLESI
                     LGVERRGAFLSVKPCIPAAWPGFSVTVRLGGAKYEIEVQNPEHRCCGVAEAELDGQGV
                     DPAAIPLKDDGKVHRVRVVIGEPVAAERSMR"
     gene            complement(9249..10148)
                     /locus_tag="VFM00_00965"
     CDS             complement(9249..10148)
                     /locus_tag="VFM00_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7496845.1"
                     /translation="MMELGAAPIQLLPAALIGLVGGALVGLTGVGAGSVIAAGLLVAF
                     PTLDAKLIVGSATAQAVVMKLFGVLARRRFRFGEGRLGIAMALGAIPMAMAGAWASSQ
                     IGGDLLRPIMAVVLLLVGLVLVAQAVPRGPDGFETYRQLSGRGERERVRDASEATGAG
                     TWDATFVDDASRAEARTLDPRRMSVAIVGALVGFIAGLTSIGTGTLFVSSLVGPLRMD
                     AHRAVAVALLAGLLTLVVSGATHLLLGHVDPVLAGGVLLGSVPGVLLGTVVSRRLSVG
                     ALRGTIGAGIAVAAVVALARLGR"
     gene            complement(10186..11418)
                     /locus_tag="VFM00_00970"
     CDS             complement(10186..11418)
                     /locus_tag="VFM00_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HET7496846.1"
                     /translation="MTTRTQAPARLASALFVGVAAWLVAAAAQAAPPAGFETRAIWVV
                     RHALTTPGRVDRVVEVATEMNANTILAQVRGRGDAFYKSDLAPLGESMGGSPEGFDPL
                     DRMIQRAHAAGLEVQAWINVYLVWSAGAPPVSPQHVVNAHPEWISIRADGTRLVEMVP
                     AEFQEEKIEGMYLSPGNPDVKRHLRDIVKEIATKYAVDGIHLDYVRYPEPMVGYDRAT
                     RTEFQRQFGVDPLQIDNPDSTTLAVIGADHLPDLRLKWIQWKRDQVTDLVRSLRQDLN
                     LLGRPIKLTAAVIADQNAALNRYLQDWPRWLREGIIDAAIPMAYSPNTATVSRQIGAA
                     VSIPTHRQVWAGIAVYNEGSRDAAEKIRRARMMGVDGIALFSYDTLLDSAGYRRNIRT
                     WAWREPTMPTPMPWRGSQ"
     gene            complement(11461..12102)
                     /gene="rplQ"
                     /locus_tag="VFM00_00975"
     CDS             complement(11461..12102)
                     /gene="rplQ"
                     /locus_tag="VFM00_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET7496847.1"
                     /translation="MRHRKDHRKLSRTREHRKALLRNLTTSLIQHERIETTVAKAKEA
                     RRMAERMITFAKRGDVAARRHVSRFVHGDDVVRKLFDTVAPWYADRNGGYTRIIKVGR
                     RLGDAGQTALLELVKSPELKERLRKEAEERIKAERAAEKGQGKKSGAKQAKGAEAPAA
                     EKGAAPAGEGEGAPRKERAPKPSRGDRLGRKGGAPKSAPHGGTRARQRGKSGD"
     gene            complement(12174..13166)
                     /locus_tag="VFM00_00980"
     CDS             complement(12174..13166)
                     /locus_tag="VFM00_00980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET7496848.1"
                     /translation="MKWKNLQMPKNVEMDDKNSTNRYGKFWIEPLERGFGVTLGNALR
                     RVLLSSLPGAAVSAVKIDGVQHEFSTLPGVKEDIPEVLLNLKQIRFKLHGDAPKMGMI
                     EVRGKSEVTAADLKVDADVEILNPELHIATLNKDGEFRGEIEIGAGRGYVSAENQANQ
                     DRPIGVLPTDSMYSPVTKVNFEVENTRIGQRIDYDKLTLEIWTDGSVIPSDALAYGAK
                     ILKDHFALFVHFEEEIEVEQEEEVDEEFLRIKALLERSVEELELSVRSSNCLKAANIK
                     TIGDLVQKSEGEMLKYRNFGRKSLKEIADILQSMNLGFGMDVSKYKLGEKIEAA"
     gene            complement(13192..13821)
                     /gene="rpsD"
                     /locus_tag="VFM00_00985"
     CDS             complement(13192..13821)
                     /gene="rpsD"
                     /locus_tag="VFM00_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET7496849.1"
                     /translation="MATYHGDKCRQCRREGEKLFLKGSRCYTEKCAIERRAYAPGEHG
                     KDRRSKETNYGQQLRMKQKARRIYRILEAQFRNYFEKAERQKGVTGENLLLLLERRLD
                     NLVYRLGFAPSRTAARQLVRHRHIEVNGRTVDVPSFSVRVGDEIRVRQGSKDLVAIQS
                     SLEANKSRDMLSWLTLDAEKLSGRMLEYPTRGNIPTKISESLIVELYSK"
     gene            complement(13842..14243)
                     /gene="rpsK"
                     /locus_tag="VFM00_00990"
     CDS             complement(13842..14243)
                     /gene="rpsK"
                     /locus_tag="VFM00_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET7496850.1"
                     /translation="MAEAASTTPKPKKKRERKVGINGIAHVQSTFNNTIVTISDMDGH
                     TVAWASAGKVGFKGSRKSTPFAAQIAAESATKEAVSLGLKKVEVWVKGPGAGREAAIR
                     SLQAAGLEISAIKDVTPIPHNGCRPPKRRRV"
     gene            complement(14254..14664)
                     /gene="rpsM"
                     /locus_tag="VFM00_00995"
     CDS             complement(14254..14664)
                     /gene="rpsM"
                     /locus_tag="VFM00_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET7496851.1"
                     /translation="MARIAGVDLPNDKRIDIGLTYIFGIGNSAARKILAITGVNPATR
                     VKSLTDEETGRLRQVIESQFKVEGARRSEVAMSIKRLMDIGSYRGLRHRKNLPARGQR
                     TRTNARTRKGPKKTAGAMRKTVAAKKPAPAKPAA"
     gene            complement(14699..14812)
                     /gene="rpmJ"
                     /locus_tag="VFM00_01000"
     CDS             complement(14699..14812)
                     /gene="rpmJ"
                     /locus_tag="VFM00_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET7496852.1"
                     /translation="MKVKASVTKICDNCKVVRRKGVVRIICKNPRHKQRQG"
     gene            complement(14855..15073)
                     /gene="infA"
                     /locus_tag="VFM00_01005"
     CDS             complement(14855..15073)
                     /gene="infA"
                     /locus_tag="VFM00_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014488934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET7496853.1"
                     /translation="MPKEEGVQVEGTVVEPLPNAMFRVELENGHRVLAHISGKMRMNF
                     IKILPGDKVTVELSPYDLSRGRIVYRYK"
     gene            complement(15073..15840)
                     /gene="map"
                     /locus_tag="VFM00_01010"
     CDS             complement(15073..15840)
                     /gene="map"
                     /locus_tag="VFM00_01010"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET7496854.1"
                     /translation="MAERIRINTPEEMEKIRESAQIVGRCLLLLGKEVRPGITTQELD
                     RIAEEYIRSQGGEPAFKGYRGYPASICASINEEVVHGIPSGKRVVKEGDVVSLDIGVK
                     KAGFYGDAARTFAVGAVSEAAQRLLEATERSLYEGIDQARPGGRVSDISSAIEREVKR
                     SGYSVVRALVGHGVGRELHEEPQVPNYGRPGEGPRLRTGMVLAIEPMVNLGGADVVTL
                     ADQWTVVSADKSLSAHFEHTVAIGEEGPIILSKIEAD"
     gene            complement(15852..16499)
                     /locus_tag="VFM00_01015"
     CDS             complement(15852..16499)
                     /locus_tag="VFM00_01015"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET7496855.1"
                     /translation="MRVVLLGPPGSGKGTHGKRLAASLGVPLIATGDILREAIAEGTR
                     LGQAAQGDVKAGRLVADATVLGIIETRLAQKDAANGFVLDGFPRTTAQADGLAALLGA
                     KRVDRVVNLVVPAETVVRRLQDRWLCPGCGAIYNMATLPPKAEGVCDLCGTRLVQRAD
                     DRPETVRARLEVYERETAPLVVYYERLGALRNVDASGSPDQVYEAVQRALGKVAA"
     gene            complement(16496..17797)
                     /gene="secY"
                     /locus_tag="VFM00_01020"
     CDS             complement(16496..17797)
                     /gene="secY"
                     /locus_tag="VFM00_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET7496856.1"
                     /translation="MLEKAQNVFRIPELKRRLLFTIALLCVYRFGGHIPTPGVNSEAL
                     VAAFASQANSLFGLYDLFVGGSFAKATVFALGIMPYISVSIIMQLLGAVVPYFEKLQK
                     EGEEGRKKITQYTRVGTVVLAAAQSFAFARFLESMGGGSVVPHPGMGFRLMTMITQTA
                     GTILIMWLGEQITERGIGNGISLIIFIGIVARFPNDIVNSVRALKAGTLSPWLAVIIV
                     AVMVAVIASVVAMTQAQRKIPVQYAKRVVGRKMYGGQSTHIPLRVNTAGVIPIIFAQS
                     LIMLPSTLALYAPGNAALQAVAGWFAHGTWVYVVLYSLTIIFFTYFYTAIVLNPTDMA
                     DNMKKYGGFIPGIRPGRKTSEYIDRILSRITLPGAIFLALIAVLPDILIRRFNVPFYF
                     GGTSVLIVVGVALDTLQQIESHLIMRHYEGFVTSGRLRGRR"
     gene            complement(17807..18430)
                     /gene="rplO"
                     /locus_tag="VFM00_01025"
     CDS             complement(17807..18430)
                     /gene="rplO"
                     /locus_tag="VFM00_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET7496857.1"
                     /translation="MAATSRADGRGTLRIGNLKPAKGATHRRKRRGLGPASGLGGTAG
                     RGHKGQRARKSGNVARWFEGGQMPIQRRLPKVGFTNIHRVPHQVVNVESLDRFAAGET
                     VNRETLKAKRLIDRSDLPVKILGTGDLSLKLTVAVDAVSGSAREKIEAAGGSVTLPEK
                     KAPRPRGVKKPARAEAKGGREAARASGRAARGSKGNKGNKSQGKVRA"
     gene            complement(18430..18639)
                     /gene="rpmD"
                     /locus_tag="VFM00_01030"
     CDS             complement(18430..18639)
                     /gene="rpmD"
                     /locus_tag="VFM00_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET7496858.1"
                     /translation="MAQLKITQVKSKIRCLKTHKRTLAALGLRHPHDTVIHNDTPQIR
                     GMIAAVDYLLRVEPHDGSAQAKGGR"
     gene            complement(18626..19168)
                     /gene="rpsE"
                     /locus_tag="VFM00_01035"
     CDS             complement(18626..19168)
                     /gene="rpsE"
                     /locus_tag="VFM00_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET7496859.1"
                     /translation="MRGRGPQQTDSEFIERVIHVNRVAKVVKGGRRFSFNAIVAVGDG
                     KGKVGVGLGKANEVSDAIRKAGEGAKKRMFEVPMIRTTIPHEVIGHFGAGRVLMKPAT
                     AGTGVIAGGAIRAVLEAAGIQDILTKSLGSGNPHNVVRATVVGLQSLRRAGEVAGMRG
                     KTVDELLGIRRKGMDGNGTA"
     gene            complement(19205..19573)
                     /gene="rplR"
                     /locus_tag="VFM00_01040"
     CDS             complement(19205..19573)
                     /gene="rplR"
                     /locus_tag="VFM00_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET7496860.1"
                     /translation="MKGKARLEGRKRRHLRIRRKVTGTAERPRLTVYRSLNHIYVQLV
                     DDLNGRTLLAASSRDGAVAELVKSAKGKVGAGKAVGKHVAAMAKEKGILRVCFDRGGY
                     LFHGRVKAVADGAREGGLNF"
     gene            complement(19601..20152)
                     /gene="rplF"
                     /locus_tag="VFM00_01045"
     CDS             complement(19601..20152)
                     /gene="rplF"
                     /locus_tag="VFM00_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET7496861.1"
                     /translation="MPSRVGKKPIPIPSGVTVARDGRSIKVKGPKGEMLFALGHGVDV
                     TVEPQEVVVAQVGGGKQALAMHGTTRAILANMIHGVTQGFTKTLEITGTGYRAAMTGK
                     KLTLQIGFSHPVEFEPPAGISITVENPTRLHVSGFDKGLVGQVAANIRGYRPPEPYKG
                     KGIKYAGEYIRRKAGKAAGGKSA"
     gene            complement(20163..20561)
                     /gene="rpsH"
                     /locus_tag="VFM00_01050"
     CDS             complement(20163..20561)
                     /gene="rpsH"
                     /locus_tag="VFM00_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET7496862.1"
                     /translation="MSVTDPIADLLTSIRNACKAKHKKCDVPASNVKAEIVRVLLREK
                     YINNYKTMDDKRQGVLRIYLKYDAKERPVLQGLERVSKPGRRVYVRRHQIPKVQGGLG
                     TALISTPSGIMTDLEAREEGLGGEYLCRVW"
     gene            complement(20605..20790)
                     /locus_tag="VFM00_01055"
     CDS             complement(20605..20790)
                     /locus_tag="VFM00_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET7496863.1"
                     /translation="MAKKSLVEKWKREPKFKVRKYNRCHRCGRSRAFLRDFGICRICF
                     RELALLGQIPGVVKASW"
     gene            complement(20817..21350)
                     /gene="rplE"
                     /locus_tag="VFM00_01060"
     CDS             complement(20817..21350)
                     /gene="rplE"
                     /locus_tag="VFM00_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET7496864.1"
                     /translation="MKEHYEKSVLPELMKRFSFANPMQAPRLRKIVVNMGVGDALVNI
                     KMLDAAVAELAQITGQRPSVRKAKKSIANFKLREGQPIGCMVTLRGARMYEFYDRLVN
                     VALPRIRDFRGVPARSFDGRGNYTLGVTEQIIFPEINYDRVEKVRGMDITFVTSARND
                     EEGQELLRLMNMPFRQR"
     gene            complement(21536..21877)
                     /gene="rplX"
                     /locus_tag="VFM00_01065"
     CDS             complement(21536..21877)
                     /gene="rplX"
                     /locus_tag="VFM00_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET7496865.1"
                     /translation="MKTPTKMPNFRRNDQVVVISGDDRGKSGRILKVLRDKNRVIVEK
                     VNFIKRHTKPRGTGRQGGILEREAPIHVSNVMHLCPKCQQGVRVTVKPDTDGKRERFC
                     GRCGETIVRAQ"
     gene            complement(21879..22247)
                     /gene="rplN"
                     /locus_tag="VFM00_01070"
     CDS             complement(21879..22247)
                     /gene="rplN"
                     /locus_tag="VFM00_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET7496866.1"
                     /translation="MVQPRSIIAISDNSGARVARVVKVLGGTRKRYGMLGDVIVVSIQ
                     DALPGAAVKKGDVHKAVLIRTRKEVRRKDGSYIRFDQNAAVLIDDQKEPKGTRIFGPV
                     ARELREREFMKIVSLAPEVI"
     gene            complement(22273..22548)
                     /gene="rpsQ"
                     /locus_tag="VFM00_01075"
     CDS             complement(22273..22548)
                     /gene="rpsQ"
                     /locus_tag="VFM00_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET7496867.1"
                     /translation="MEGAASPERGRRKFMTGRVVSDSMQKTVVVTIERLVKHEEYGKY
                     VRRRSRFKVHDEKNECKVGDVIRFMETRPLSKDKRWRLVDFVQRVER"
     gene            complement(22548..22973)
                     /gene="rpmC"
                     /locus_tag="VFM00_01080"
     CDS             complement(22548..22973)
                     /gene="rpmC"
                     /locus_tag="VFM00_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET7496868.1"
                     /translation="MRDLKAEKIREMTEDEILTKIRELKEELFHLRFRNQMRQLQNPL
                     LLREKKRDVARMHTILAEHKSGIRPLAGHAAGTVAATGAAPSEARSAAPAARPKARAG
                     AKKKTAKAKPKKAAAKSRPSASKAKAKSKPAAKRKAKKS"
     gene            complement(22975..23397)
                     /gene="rplP"
                     /locus_tag="VFM00_01085"
     CDS             complement(22975..23397)
                     /gene="rplP"
                     /locus_tag="VFM00_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET7496869.1"
                     /translation="MLVPKRVKHRKQMRGRRKGKAYTGGTVAFGEFGLQAMEAAWITN
                     RQIEAARVAITRYIKRGGKMWIRIFPDKPVTVKPAETRMGKGKGSPEFWVAVVKPGRV
                     LFELEGIEERLAREAMKLGASKLPIKTKFVERQTGAGS"
     gene            complement(23410..24087)
                     /gene="rpsC"
                     /locus_tag="VFM00_01090"
     CDS             complement(23410..24087)
                     /gene="rpsC"
                     /locus_tag="VFM00_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET7496870.1"
                     /translation="MGQKTNPIGMRLGIIRTWDSRWFARKDYPALLKEDIVIRRYLKK
                     RLSQAGISRIAIQRTASKVTINISTARPGLVIGRRGQQVDQLRDELQHLTMKKEIFLN
                     IDEVKKPDMDATLVGEHVARQLEQRVSFRRAMKKAIASTMRSGAGGVRIVCSGRLGGA
                     EMARTESYRDGRVPLHTLRADIDFARSTARTIYGAVGVKVWIFKGEILERKEPGEASP
                     ATPARTA"
     gene            complement(24272..24598)
                     /gene="rplV"
                     /locus_tag="VFM00_01095"
                     /pseudo
     CDS             complement(24272..24598)
                     /gene="rplV"
                     /locus_tag="VFM00_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            complement(24621..24914)
                     /gene="rpsS"
                     /locus_tag="VFM00_01100"
     CDS             complement(24621..24914)
                     /gene="rpsS"
                     /locus_tag="VFM00_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET7496871.1"
                     /translation="MSRSIKKGPFVDPKLLKKIEGMNKANERRVVKTWARRSTISPEF
                     VGHTLAVHNGNKFIPVYITENMVGHKLGEFAATRIFRGHGSSEKAAATPAPAK"
     gene            complement(24944..25774)
                     /gene="rplB"
                     /locus_tag="VFM00_01105"
     CDS             complement(24944..25774)
                     /gene="rplB"
                     /locus_tag="VFM00_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET7496872.1"
                     /translation="MALRKFRPLTPTQRFRTVASFDDVTRQGPEKGLTQHLPKKGGRA
                     NTGHTSAWLRGGGHKRLYRVIDFKRDKRGVPAKVAHIEYDPNRSARIALLHYADGEKR
                     YILAPQLLKVGDTVLSGNDAEPRPGNAMELGFVPLGTFIYNVELVPGKGGQLSRSAGT
                     FCQLMAKEGDYAALRLPSGEMRRVPLRSWCTIGQVGNEDHQNIVIGKAGKSRWMGRRP
                     NVRGVAMNPVDHPMGGGEGKSSGGRHPCSPWGLLAKGKKTRKTKLSDRLILRRRKAKS
                     "
BASE COUNT         3703 a         9279 c         8493 g         4397 t
ORIGIN      
        1 gttctttcga atcggcgtcg cgcccgatca gaatgttcgg cggtgcggcc ccacgtgtca
       61 ctcgctcaac ccccgtcatc ggatcgaccg tggacgacac aagaatagcc gtgacgtgcc
      121 gaaagttcgc gttcgatgtt ctcgaatgca tcctgggcag gaatcatgac gaccgtgtct
      181 cgcggcaagt tccttcgctg cgaagcgtcc ggtgccgtaa ggccccaatt ctagcagcct
      241 tgcgggctcg gccttttccc acatgcgcct ttccctaaag gacacactcg gggcgaaggc
      301 gatacggtga ctgagtctgc gttcgtcgcg cttcgactag aagcctatgc gtttgcaggg
      361 agccagagag gacgagaacg cattggttcg acaaacgcga gagccccggc gcatcgctgc
      421 accggggccc ctctctattc ttctgccgcg gtctctaggc gcgcggcccg tgtctggctc
      481 ctgaggtagg attcgaacct acaaccctcc ggttaacagc cggatgctct accattgagc
      541 tactcaggaa tggaacggca actgcttgtc ctgcaatgac ctccgcgccg agcggccgca
      601 cacactgcat cgacccggag ccgaatagtc tacctcatcg agcgttcggc cgcaaccggc
      661 tccccgatca cgaccctcac gcggtgcacc ttcccgtcgt ccttcagcgg gatcgccgcg
      721 gggtcgacac cctgcccgtc cagttcggcc tccgcgacgc cgcagcagcg gtgttcggga
      781 ttctggacct cgatctcgta tttcgcgccg cccaagcgga ccgtgaccga gaacccgggc
      841 caggcggcgg ggatgcacgg cttcaccgag aggaacgcgc cgcggcgctc cacgcccagg
      901 atcgactcga gccccgcgcg atacatccag ctggccgaac cggtgtacca cgtccatccg
      961 ccgcgcccga cgtgggccgg atgggtgtag acgtcggccg cgaccacgta gggctcgacc
     1021 ttgtagcgtg cgacgtcccc ctccgaccgc gtgtggttga tcggattcaa caggtggaac
     1081 agctcgaccg cctcgtcgcc gctccccatg gccgccacgg ccatcacgac ccagacggcg
     1141 gcgtgcgtgt actggccgcc gttctcgcgg acgccgggga tgtatccctt gatgtagccg
     1201 ggatccagcc gggacgcgtc gaaggggggc gtgaggagca tgacgacgct ggcatcgcgc
     1261 cggatcagat ggctccgcac ggaatccatc gcgcgctcgg cgcgcgcggg aggcgccacg
     1321 ccggagagca gggcccagga ctggcagatc gaatcgatcc ggcactcgtc gttctgggcc
     1381 gagccgagcg gcgtgccgtc gtcgaagtag gcgcgccggt accactcgcc gtcccacgcc
     1441 tgctccatca tgctggcgat ccggccggcc tcgccgcggt agcgcgcggc gcgatcgggg
     1501 tcgccgcgcg tctcggcgag agcggcgaac tcgcggagga tcatggccag gaaccatccg
     1561 agccagacgc tctccccgtg gcccagccgc ccgacgcggt tcatgccgtc gttccaatcg
     1621 ccgtcgccga tcagcggcag cccgtgcggg ccggaggtca ggctgcactc gatcgcccgc
     1681 acgcaatgtt cgtacagcgt cccatcctgg gcggacgtct gcggccggcc atacgcttcg
     1741 tgttcttccg gaccgagcgg cggcgcctcc aggaacggaa tccgctcctc cagcacgctc
     1801 gcgtcgccca ccgacttgag atagcgcaac acgacgaagg gcagccacag ccggtcgtcc
     1861 gagcagcgcg tccggacccc tacccccgaa ttcgggtgcc accagtgctg aacgtccccc
     1921 tccacgaact gccgccccgc ggcccggagc acgtgctcgc ggaacagggc cggatcggcg
     1981 aacggcagcg ccatgacgtc ctggagctgg tcgcggaatc cgtaggcgcc gccgggctga
     2041 tagaacgccg tccgcgccca gatgcggctg gagaccgact cgtagagcag ccagcggttc
     2101 atgatcaggt cgaaggagtc gtccggggtg cgcacctgga cggctccgag catcccatcc
     2161 cagaactctt cgacttcctg gagggccgag agcgcggccg gcaccgatcc gaagcggcgc
     2221 gccatcgcga gcgcctcgtc cttgtccgcg ccctccccga gcagcaggat gacctgcttc
     2281 gtctctcccg gctccaggtc cagcgagacc tggagcgcgg cgcacgcatc gagccccgcg
     2341 ccgaacgcat tcgacagctc ctcgcgttcg agcgcggcgg ggcgtgcgac ggagccgttc
     2401 cggccgagga actcggtccg atcgccgctc gccgagcgca ccggaacgcc ggcatgcgcg
     2461 aaggcgacgc gcccgcggaa atcctggttg tagggattct cggccagcac cgtggcggtg
     2521 gccggatcga actcggtgcg gacgtgcacc tgctcccgtt cgcgcggcgg gccgaggcgc
     2581 cactcctggt agccgaacac gctcacgcgc cgcgcgcgct tggtctggtt cgccagcgtg
     2641 agcagcgcga accggaccgg ctcggagcgg tggacgaaca ccgccagctc gtgacggatg
     2701 ccgcgttcgc gatggacgta gcgggtcacg cccacgccgt ggcgcaccag ccatcggccc
     2761 gcgtcgtccc ggcggcgcat cgggccgggt gtggcgcccc atgcggcccc gctctcgtcg
     2821 tcgcgcaggt agatcgcctc gccggtcgct tcggtcgccg gatcgttggc gaagggcgtg
     2881 agccggttct cgcggctgtt ctccgaccac gtataggaga gtccggccgc ggtcacgacc
     2941 gaaccgaatc gcggattggc gagcacgttc gcccagggaa gcggcgtctc gtcggcgccg
     3001 ttcagcacga cggcgtattc ctggccgtcg agcgtgaagc cgccgcgacc gttccagagc
     3061 aggagctcgg ggacttcgat gtcctccgaa acttcttcgc ctccggaggc cgccccccca
     3121 ccctcgatcg ccagcgccgg cggccagacc ggatcggggt aggcccgatt gagctgatcc
     3181 tcgagactcc ccgcgtcgcc gcggaggacc gcacgcgcga cggcggcgag cagggtccgg
     3241 tcggcctccc ccatcccatc gccggtgaga aggaacgccc cgccgggctt gtccttccac
     3301 gcggcccagg gcccgctgtc gagaagcccg ttcagctgct cgtggatctc gtttcgatag
     3361 ctgaccgggt ggtcgttcag gatcaggagg tcggcgacca gccccttcag gcgccaatag
     3421 tcctgggcgc ggagcacctg gctcacgagc ccgatgtcgt cgggctcgag cacgcggacg
     3481 aggaggatgg gattgtcccc ggagatgccg tgcccccaga ggccgggctg gccgagtacg
     3541 ttccgcgcca ggatgtccgc ggccggcccg agcgagcggt ccacgccaaa gacgcgcgag
     3601 gcgagccgct cgtagagctg cgcctcctcg acccggatgc cgaggtggcg gagcgagatg
     3661 gagaggtggg tcgccgccag ggcgaacgtg cgcgccgccg agttcggatc cgcgtatttc
     3721 atgcagagcg cgacggcggc gtcgcggtcg gaggcgacgc cggtcgcgaa ggacaggcgc
     3781 gcgaaggctc ccggcgcgag ccggatgcgc tgccgaaggc tcaggatggg atcgagcgtg
     3841 gcgcccacgg tgcccgagag ggcccggccg tccagcgcga tcggatcgtc cgtgccgcgg
     3901 ccgcggccca ggaaccgcac gcggtcggtc tcccattcca cggcgctctg catccggcct
     3961 tccatgctga gcacgtggac cgcccacgcg cgcggatcgt ccgacgagcg ggggcgccgg
     4021 gagcagagga gcgacgccgt ctccggccgg cactcggtct ccaagaaaag cttcccgaac
     4081 acgggatggg ccaggtcgtc ggcctgcggc gcgagcacca cctcgtcgta gctcgtgacc
     4141 tcgatctcgc gcaggtaggg gcttcggttc gtcagggaga gccgccggac ctcgacgtcg
     4201 tcctcggccg agaccgcgat ctccagcttg gactggatcg cctccaggcg ctgctcgatg
     4261 acgacccggt cggggaggaa cgtggcccgg tattcgtccg gctcgcggcg gatcggctga
     4321 tacgcggccg accacacggc cccgctccgc acgtcgcgga gatagaggca ctggctggcc
     4381 ggatccaggg tcgagtcctc gcggcgccgc gtgaccgtga tgccacggca ggagctgctc
     4441 ccgccccccg aattgctcac ggtggcgaca tagcggccgt tcgagaggaa ggccgtatgc
     4501 ggataacggg tatgcggcgt gcggaagcgg cgcggcagca tcgggaggac cggaaccgca
     4561 aggtgcgtca tctcgaccgg ccggggccgc gtcacgggga cgaagcgcgg gacccgctct
     4621 tgaaggagga gctcggtcgc ctggacgcgc gcttcgctgt ggaagcggct caccatcgga
     4681 tcgtggagca ccgcgttcga cagcgccacc accgacattc cctggtgatg cgcgaagtac
     4741 gcctggacca cggccggctt cgggtggcca tcggcccggg cgaccggttt cccgtctccc
     4801 tcctccgcct cgcgcggcgt gtaatcgatc gcttcgtaga agccgaacgc tccctcggcg
     4861 ccctcccgtg tcaggcgcct cagattggcg gccgcctcga cgggatcgac catcgcggcg
     4921 agcgcggtgg cgtagggagc gatgaccagc tcttcggaga gtccgcgctt cagccccagc
     4981 tcgggcacgc cgaacgcctt gtactggtag ttgccgtgcc ggtccacctc gttgaacgcg
     5041 gattcggaga tcccccacgg cacgccgcgc atccgcccgt actggatctg ggcgcgcacc
     5101 gcggagtcgg acgatcgctg gagtaacgtg tcgggatagt tgcgcatcag gaggaggggc
     5161 atcaggtact cgaacatcga cccgctccag gagaccaggg tgggcacgcc ggcgatgccg
     5221 acgagcgcgc ggcccagctg gaaccagtgc tccggaggca cgtctccctt cgcgatcgcc
     5281 acgaagctcg cgagacgcgc ttcggaggcc aggaggtcgt aataggagac gtccaggcgc
     5341 cccggcccct ccatgtcggc cagacggtag ccgatcgaga agatgcggcg gtcccggtcg
     5401 tagaggaatc ggaagtccat ctcgtcggcc agcgcctcgg cgcgagccgc gagcgctgcc
     5461 cgccgctccc gccacgactc gggcagatcc cccgccttcg gatgacgcag gagccggacg
     5521 agcgtctcgc cccagaaagc ggcctcctcc gcttccggtc cctcgaggcg atcgagatcg
     5581 gcgacctcga gcgcctgcgc cagcgcgcgc cccgcgccgt ccaggcgcgg cgagtcggga
     5641 cgggcggcga agcggcgcac ctcgtacgcc tccacctcgc gaagcgcggg ccggagccgc
     5701 tcgcggatcg actcggcgga cgcctcgggc aggatccgat ccaggctctg tcgcagaagg
     5761 gccagcgtgt cggcgacggc ggcggcgtgc cggaatcctt ccgcgggcgc cggctccagc
     5821 cgccgaagac ccgccgcgag cgccatgagc gagccggcga gattcgcgct gtccacggtg
     5881 gagacgtaac gcggctggag cggtgcgagg ctcagggtgt cgtaccagtt cagcagatgc
     5941 ccttcgtgct tctccagccc gtccagcgtg ttcaggatcc gctccacccg gtcgagcacc
     6001 tcgggcgcgc cgatgaagcc caggtcgcgg gccgcgagcg tggcgaggag cgccatgccg
     6061 atgttggtcg gcgacgtgcg atgggccacc ttccggtccg gcgtctcctg gacgttgtcg
     6121 ggcggaagcc agtggtcctc ctcgcccgcg aacgtatcga agtagcgcca ggtgttacgc
     6181 gcgatgcggc ggagctcgac ccggtcatgc ggctcgagcg cgaccacgcg gcttcgcgcc
     6241 ggaagactga gccagtaggc gatgagcggc gagacgagcc agagactgag cagcgggagc
     6301 gcggcgccga gcgcctcgat ccgaaacgcg accgtcgcaa ccaggatcag gaccgccacg
     6361 gcggggccgg cccacatgcc gttggcgaac gcgaccagct ggtcctttcc cgagaggccg
     6421 gcggagcgcg cggcggcggt ggccgcggtc tcccactcca gcatcttccg ccgcgtgaag
     6481 aagagccgca ccagcgtgac cgcgatggcg tggagcatct cgtacgactg gtaggcgagc
     6541 agggtgatgt ccaggaacac ctgggcgccc gccgtctgga actcctcgcc caggtcgcgg
     6601 aggaagaccg ggaccggctg ctggggctcc gggcccttca ccatgcgcac gatcttggag
     6661 tagagcggga agcacatccc caggatcacc gcgaccgtcc atacccacgg ggggccgggc
     6721 agccaggtcc aggccgacgc caggagcgcg agcgtcgccg gcccgaggac gctgcggcgc
     6781 agattgtcga ggatcttcca gcgcgcgatc agcggcagac ggttccgctc caggctgccg
     6841 cgcgtgggca cccaggggaa gagccacgcg aggatctgcc aatcgccgcg cacccagcgg
     6901 cgctggcgcc ccgcgtgcgc caggacgctc gaggggaagt cgtccaccac ctcgatgtcc
     6961 gataccagcc cggtccgggc gtagatcccc tcgaacaggt cgtgcgagag gagcgcgttc
     7021 tcgggaacgc gcccctccag cgcggccatg aacgcgtcga cgtcgtacag ccccttcccc
     7081 gtgaagattc cctcgctgaa caggtcctgg taagtgtcgg agacggcggt cgtgtagatg
     7141 tcgacgcccg tgtgcccggc gtagacgcgc gcgaagagcg atccggcggc gctcgccatg
     7201 gtgacgctca cgcggggctg gaggattccg tagccgcggg tcacgcgacg gaggaccgga
     7261 tcgaagcgtg ggcggttcag cgggtgctgg atgatgccga tcagcttgcg cgcggtctcc
     7321 cggggaagcc gcgtgtcggc atccagcgtg atcacgtagc ggaccgacgt gaggatggag
     7381 gggtccccga ccaccgcctg gaacgacgaa gggtggccgc gcaggagcgc attgaactcc
     7441 tcgatcttcc cccgcttccg ctcccagccc atccagacgc cttcgcgctc gttccagcgc
     7501 cgcggccgat gaaagagata gaaccggtcc ctggccccgt tcccgtaccg cgcgttcaac
     7561 gcctcgatgc ccgaggtgac ggcggcgagg accgcggcgt cctcggggcg ctccggctcg
     7621 gtcgcgtccc ggaaatcggt gagcagcgcg aagtggatgt ggcggtcctc gttccccagg
     7681 gcctgcacct cgaggtgctc gacgtgcgcg cgcgcggcgg ccacgctgcc gaggaggacc
     7741 ggcacgacca cgatcgtgcg cgcctcgtcc ggcacgccgt gctggagatc gaggcggggg
     7801 agccggcgcg ggtgggccag ccggtgcacc atccgctgca tgaagagcac cgcgacgtgg
     7861 ctcgccggaa tgagggtcag gagaccgatc cacggcaaca tcgcgaccgg cgcgccccag
     7921 gcgcgcgacc acgccacggc caggccgacg ccgagcgcgg tcaccagcgc gatcgatccg
     7981 aggtagaaga gggtacactc ggcgaaaagg aggcggcgga tccgctgcgc gggccggggg
     8041 gtgtaggcga cgtcgacctc gaagtcgcgc cggccgccgc cgaccaggtg gtagcccacg
     8101 tgcgtcgcgg gctccccttc gccgcgctcc cccgcctggc gcgcgctctc cacggcgcgg
     8161 agcgccacgc gcacctgcgc ctcgccgctg ggctcggaga gctcctccac cgactggcgg
     8221 tagcggtcgc ggctcgggaa atccatccgg ccatagaccc ccgccgggtc gcgccgcagg
     8281 atctgctcca tgaggctcac ctgctcgatg gtgcggttcc agtcggtcgt ggcgaccagc
     8341 cggagcgcgg tgatcgagtt ccccatcgag gcctgcccca tcgtctgccg ctggtgctcg
     8401 gagcgcagcg cctcgtcgaa cgtgagcccg gcctcgtgca ggcgccggtc cagctccacc
     8461 cgcagctctc cgacgcgcgg gcccagctcc cgcatccgct ggaccagctg caccacgaac
     8521 ccgttcgaga ggttgcccgg gagcggagga atcggatcgg tggggcggat cgtctcgaag
     8581 cgcgtgaagt aggcgtcggc gtcggcctcg ccctcgcggc tctccatgat ctctccggcc
     8641 agccgccgga tgttctcgat gaggcagagc ttgagcatgc tgggccaggc ccacagctcg
     8701 cccagcgtga gcggcgccac cgcctggtag gcgccgatga actgcgtcag acggtggagg
     8761 tcgaagcgcg cgtcgctgtg gcggatgaac tcgagcgcca tggcgtggac gcgggccgtg
     8821 cccgcgagat cgcgcgaggc gagcttcggc agctcgagat agtagcggtg gggaaggttc
     8881 ttccggacct cgaccgcttc cgtctcgacg agatggaagt tgtcgagcag ccactcggcg
     8941 gcgggcacga ccggctcccc gcggcggacg tcgttcgcca gctcgcggta ggcgtggcgg
     9001 aggacgcgcg cgttctcgtg gagccgggga aggaggcggc gggcgcggcg gcggggattt
     9061 cgggcgacgg tgtagctggc ggcgagcacg cgggcccgct cctccaggtg ctcgacgctg
     9121 aggagctccc cgcgcagcgg ctcgcggtcc gagccgggtg ctctacggct cggatcgcgc
     9181 cattttccga agacccagat ggtgcccatc gaccccttcc cggaggcagc ctttcatcgc
     9241 cgcgtctcct agcgcccgag ccgcgccagg gccaccacgg cggccacggc gatgcctgct
     9301 ccgatcgttc cacgcagcgc gcccacggag agccgccgcg acaccaccgt gcccagcaag
     9361 accccgggca ccgagcccag caggacccct ccggccagga cggggtccac gtgaccgagc
     9421 agcaggtggg tcgcgccgct cacgacgagc gtcaagagcc cggccaggag cgccacggcg
     9481 acagcgcgat gcgcgtccat ccgaagcggc cccaccagcg aggagacgaa gagcgtcccc
     9541 gtgccgatgc tggtcagtcc cgcgatgaac ccgacgagcg cgcccacgat ggccacgctc
     9601 atgcgccgcg ggtccagcgt gcgcgcctcg gcccgcgagg cgtcgtccac gaacgtcgcg
     9661 tcccacgttc cggcgcccgt cgcctcggat gcgtcgcgca cgcgttcccg ctcgccgcgt
     9721 cccgacagct ggcgataggt ctcgaagccg tcggggcccc gcgggaccgc ctgcgccacc
     9781 agcaccagcc ccacgagcag gagcacgacg gccatgatgg gccgaagaag gtcgccgccg
     9841 atctggctgc tcgcccacgc gccggccatc gccatgggga tcgcgcccag cgccatggcg
     9901 atgcccaggc gcccctcccc gaatcggaag cgccggcgcg ccagaacgcc gaacagcttc
     9961 atcacgaccg cctgggcggt ggcggagccg acgatcagct tggcgtcgag cgtcggaaaa
    10021 gcgaccagga ggccggcggc gatcaccgat ccagccccca cgccggtcag gcccacgagc
    10081 gcacccccca cgagcccgat cagggccgcg ggcaggagct ggattggcgc cgctcccaac
    10141 tccatcattc tacgctcttt ccgttccggc gctcgtctct tcccgtcact gcgagcccct
    10201 ccagggcatc ggggtcggca tggtcggctc cctccaggcc cacgtccgga tgttccggcg
    10261 gtaccccgcg ctgtcgagca gcgtgtcgta ggagaagagc gcgatcccgt ccacgcccat
    10321 catgcgcgcg cgccggatct tctccgcggc gtcgcgggac ccctcgttat agacggcgat
    10381 tccggcccaa acttgacggt gcgtgggaat ggacaccgcc gcgccgatct ggcgggagac
    10441 ggtggcggtg ttggggctgt acgccatggg gatcgccgcg tcgatgatcc cctcccgcag
    10501 ccaccggggc cagtcttgga gatagcggtt cagcgccgcg ttctggtcgg cgatgaccgc
    10561 tgcggtcagc ttgatcggcc gccccaggag attcaggtcc tgccgcaggg agcgcacgag
    10621 atcggtgacc tggtcccgct tccactggat ccacttgagg cgcaggtcgg gaaggtgatc
    10681 ggcgccgatc accgcgagcg tcgtcgaatc ggggttgtcg atctgcagcg gatccacgcc
    10741 gaactgccgc tggaactcgg tgcgcgtggc gcggtcgtag ccgaccatcg gctcggggta
    10801 gcgcacgtag tcgaggtgga tgccgtccac ggcgtacttc gtcgcgatct ccttcacgat
    10861 gtcccggagg tggcgcttca cgtcgggatt cccgggcgag agatacatcc cctcgatctt
    10921 ctcttcctgg aattcggcgg ggaccatctc gaccagccgc gtgccgtcgg cgcggatgga
    10981 gatccactcg gggtgggcgt tcacgacgtg ctggggcgag accggaggcg cgcccgcgga
    11041 ccagacgagg tacacgttga tccacgcctg cacctcgagg ccggcggcgt gcgcccgctg
    11101 gatcatccgg tccagggggt cgaatccctc gggagagccg cccatcgact cgcccagcgg
    11161 ggccaggtcc gacttataga aggcgtcccc gcggccgcgc acctgggcga ggatggtgtt
    11221 ggcgttcatc tcggtcgcca cctcgaccac gcggtccacg cggcccgggg tggtcagcgc
    11281 gtggcggacg acccagatgg cgcgggtctc gaagccggcc ggaggcgccg cctgcgcggc
    11341 cgccgcgacg agccaggccg cgaccccaac gaaaagcgcc gacgcgagcc gcgccggcgc
    11401 ttgagttctc gtcgtcaatg acccccagcc cgccctgcct cccatcacgg gctcgcggtg
    11461 ctaatccccg ctctttcccc gctgacgcgc gcgcgttccg ccgtgcggcg cggacttcgg
    11521 cgcgccgccc ttccgtccca ggcgatcgcc gcgcgaaggc ttcggcgccc gctccttgcg
    11581 cggagcccct tcgccctcgc ccgcgggcgc ggcgcccttc tcggcggccg gcgcttcggc
    11641 gcctttggcc tgcttcgcac ccgacttctt accctggccc ttctccgccg cgcgctcggc
    11701 cttgatccgc tcctcggcct ccttgcgcag ccgctctttc agctcgggcg acttgaccag
    11761 ctcgagcagc gcggtctgtc ccgcgtcgcc cagccggcgg cccaccttga tgatgcgcgt
    11821 gtagccgccg ttcctatcgg cgtaccaggg cgccacggtg tcgaacagct tccgcaccac
    11881 gtcgtccccg tgcacgaacc gggaaacgtg gcggcgcgcg gcgacgtcgc cgcgcttggc
    11941 gaaggtgatc atccgctcgg ccatccggcg ggcctctttc gccttggcga ccgtggtctc
    12001 gatccgctcg tgctggatga gcgacgtcgt caggttgcgc aggagcgcct tccggtgctc
    12061 gcgcgtgcgg ctcagcttgc gatggtcttt gcgatgacgc atggcttcct tcgtcctatt
    12121 cggttacggg cgggctaaag aacaggagtt ccgcggggct gcggccccgc ggatcatgcc
    12181 gcttcgatct tctcgcccag cttgtacttc gacacgtcca tgccgaagcc caggttcatg
    12241 ctctggagaa tgtcggcgat ctccttgagc gacttccggc cgaagttccg gtacttgagc
    12301 atctcaccct cggacttctg gaccaggtcg ccaatggtct tgatgttcgc ggccttcagg
    12361 cagttcgacg agcgcacgga cagctccagc tcttccacgc tccgctcgag cagggccttg
    12421 atgcgcagga actcctcgtc gacctcttcc tcctgctcga cttcgatctc ctcttcgaag
    12481 tgcacgaaga gggcgaagtg gtccttgagg atcttggcgc cgtaggccag cgcgtccgaa
    12541 gggatcacgc tgccgtcggt ccagatctcc agggtcagct tgtcgtagtc gatgcgctgc
    12601 ccgatccggg tgttctccac ctcgaagttc accttggtca cgggcgagta catggagtcg
    12661 gtgggcagca cgccgatcgg ccggtcctga ttggcctggt tctcggccga gacgtacccg
    12721 cgtccggcgc cgatctcgat ctccccgcgg aactcgccgt ccttgttcag ggtggcgatg
    12781 tgcagctcgg gattcaggat ctcgacgtcc gcgtccacct tgagatccgc cgccgtcacc
    12841 tcggacttcc cgcgcacctc gatcatgccc atcttgggcg cgtcgccgtg cagcttgaag
    12901 cggatctgct tcaggttcaa aaggacttca gggatgtctt ccttgacgcc cggaagggtc
    12961 gagaactcgt gctgcacgcc gtcgatcttg acggcggaga ccgccgcgcc gggcaaggac
    13021 gacaggagca cgcgacggag cgcgttcccc agcgtcacgc cgaagccgcg ctcgagcggc
    13081 tcgatccaga acttgccgta gcgattggtc gagttcttgt cgtccatctc gacgttcttg
    13141 ggcatctgaa gattcttcca cttcatttct ctatcctccg gggcccgggg gctacttcga
    13201 gtacaactcg acgatgagcg actcggagat cttggtcgga atgttgccac gcgtcgggta
    13261 ctcgagcatc cggcccgaca acttctcggc gtccagcgtc agccagctca gcatgtcgcg
    13321 cgatttgttc gcctccagcg aggactggat ggcgaccagg tccttgctgc cctggcgcac
    13381 gcggatctcg tcgcccacgc gcaccgagaa ggagggcaca tccaccgtac ggccgttcac
    13441 ctcgatgtgg cggtggcgga ccagctggcg ggccgccgtg cgtgacgggg cgaatcccag
    13501 ccggtagacg agattgtcga gacgacgctc cagcaagagc agcaggttct cgccggtgac
    13561 gcccttctgg cgctccgcct tctcgaaata gttgcggaac tgcgcctcga ggatccggta
    13621 gatccgccgg gccttctgct tcatccggag ctgctggccg tagttcgtct ccttggagcg
    13681 gcggtccttg ccgtgctctc cgggggcgta cgcgcggcgc tcgatggcgc acttctccgt
    13741 gtagcagcgg gaccctttca ggaacagctt ctcaccttcc cggcggcact gccggcactt
    13801 gtcaccgtga tacgtcgcca taatgaagcg gtacctcccg tttagacgcg ccgccgcttc
    13861 ggcgggcgac aaccgttgtg cggaatcggg gtgacgtcct tgatggccga gatctcgagc
    13921 ccggccgcct ggagcgaccg gatggccgct tcacggccag cgcccggtcc cttcacccag
    13981 acttccacct tcttcagacc cagcgagacg gcttccttgg tcgcggactc ggccgcgatc
    14041 tgcgccgcga agggggtgct cttgcgcgac cccttgaacc ccaccttgcc ggcgctggcc
    14101 caggccacgg tgtggccgtc catgtcggag atggtcacga tggtgttgtt gaacgtcgac
    14161 tggacgtggg cgatgccgtt gatgccgacc ttccgctcac gcttcttctt gggcttcggg
    14221 gtggtgctgg ctgcttccgc cactcagttc tccttaggct gccggcttcg ccggcgcggg
    14281 cttctttgcg gccaccgtct tgcgcatggc gcccgcggtc ttcttcggtc ccttccgcgt
    14341 ccgcgcgttc gtgcgggtgc gctgcccgcg cgcgggaagg ttcttgcgat gacgcaggcc
    14401 gcggtagctg ccgatgtcca tgagccgctt gatggacatg gcgacctcgc tccggcgcgc
    14461 gccctcgacc ttgaactggg attcgatcac ctggcggaga cggcccgtct cctcgtcggt
    14521 gaggctcttc acgcgcgtgg ccggattcac gccggtgatg gcgaggatct tccgcgcggc
    14581 ggaattcccg atgccgaaaa tgtacgtcag gccgatgtcg attcgcttgt cgttcgggag
    14641 atccaccccc gcgatacgtg ccaacgcgtg cctcctcgtg atcccgcggc gcccggggct
    14701 agccctggcg ctgcttgtga cgcgggttct tgcagatgat ccggacgacg cccttccgcc
    14761 ggacgacctt gcagttgtcg cagatcttgg tgaccgatgc cttgaccttc atgacccgtt
    14821 cccttccgcg cggccgccga cggcccgctg cccgctattt gtaccggtag acgatgcggc
    14881 cccgcgacag gtcgtagggc gacagctcca ccgtcacctt gtccccgggc aggatcttga
    14941 tgaagttcat ccgcatcttg cccgagatat gcgccaggac gcggtgcccg ttctccagct
    15001 ccacccggaa catcgcgttc gggaggggct cgacgacggt cccttcgacc tggactcctt
    15061 cttccttcgg cattagtccg cctcgatctt ggagaggatg atgggaccct cctccccgat
    15121 ggcgaccgtg tgctcgaagt gcgccgacag gctcttgtcc gcgctcacca ccgtccattg
    15181 atcggccagc gtgaccacgt ccgcaccgcc gaggttcacc atcggctcga tggcgagcac
    15241 catcccggtc cgaagcctgg gcccctcgcc gggacgaccg tagttcggga cctgcggttc
    15301 ctcgtgcagc tcccgtccca cgccgtgccc caccagggcc cgcaccacgc tgtagccgct
    15361 gcgcttcacc tcgcgctcga tagccgagga gatgtccgaa acgcgcccgc cgggccgcgc
    15421 ctgatcgatg ccttcgtaca gcgatcgctc cgtggcttcc agcaggcgct gggcggcctc
    15481 gctgacggcg ccgacggcga acgttcgcgc ggcatcgccg tagaaccccg ccttcttcac
    15541 gccgatgtcc aacgacacga cgtcgccttc cttcaccacg cgcttcccgc tcgggattcc
    15601 gtgaacgacc tcctcgttga tcgaggcgca gatgctggcc gggtacccgc ggtacccctt
    15661 gaacgccggc tcaccgccct ggctgcggat gtactcctcg gcgatccggt ccagctcctg
    15721 cgtcgtgatc ccgggccgca cctctttgcc caggagcagg aggcaccggc ccacgatctg
    15781 ggcgctctcc cggatcttct ccatctcttc cggagtgttg atccggatgc gctcggccat
    15841 cgtcgtgccg ctcaggcggc gacctttccc agggcgcgct gcaccgcctc gtagacctgg
    15901 tcgggcgagc cggacgcgtc cacgttccgg agcgcgccga gccgctcgta atagacgacg
    15961 agcggcgccg tctcccgctc gtacacctcg agccgcgccc gcaccgtctc cggccggtcg
    16021 tcggcgcgct gcacgagcct cgtgccgcac aggtcgcaca ctccctcggc cttgggcggc
    16081 agcgtcgcca tgttgtagat ggcgccgcag cccgggcaga gccagcgatc ttgaagtcgc
    16141 ctgaccacgg tctcggccgg gaccaccagg ttcacgaccc ggtcgacccg cttcgccccc
    16201 aggagcgccg cgagcccgtc ggcctgcgcg gtcgtccgcg gaaagccgtc gagcacgaac
    16261 ccgttggccg cgtccttctg ggccagccgc gtctcgatga tgccgaggac ggtggcgtcg
    16321 gccacgagcc gccccgcctt cacgtccccc tgcgccgcct gccccagccg cgtcccctcg
    16381 gcgatcgcct cccggaggat gtcccccgtg gcgatcagcg gcacgcccaa ggatgcggcc
    16441 agccgcttgc catgcgttcc cttaccgctc cccggagggc cgaggagcac caccctcatc
    16501 ggcgaccccg cagccgcccc gacgtcacga acccttcgta gtgccgcatg atgaggtgcg
    16561 actcgatctg ctgcagggtg tcgagcgcca cgcccaccac gatcagcacg ctggtgccgc
    16621 cgaagtagaa cggcacgttg aagcggcgga tcaggatgtc cggcagcacc gcgatgagcg
    16681 cgaggaagat cgcgcccggc agcgtgatgc gggacaggat ccggtcgatg tattcggacg
    16741 tcttgcggcc cggtcggatt cccgggatga agccgccgta tttcttcatg ttgtccgcca
    16801 tgtccgtcgg gttcaggacg atggcggtgt agaagtaggt gaagaagatg atcgtgagcg
    16861 agtagaggac cacgtacacc caggtcccgt gcgcgaacca ccccgccacg gcttggagcg
    16921 ccgcgttccc cggcgcgtac agcgccagcg tgctcggcag catgatcaac gactgcgcga
    16981 agatgatcgg gatcacgccc gccgtgttca cgcggagcgg gatgtgcgtg ctctgcccgc
    17041 cgtacatctt cctcccgacc acgcgcttgg cgtactgcac cgggatcttc cgctgcgcct
    17101 gggtcatcgc cacgaccgac gcgatcaccg ccaccatcac cgcgacgatg atgaccgcga
    17161 gccagggcga gagcgttccc gctttcaggg cgcgcaccga attcacgatg tcgttcggga
    17221 agcgcgccac gattccgatg aagatgatga gcgagatccc gttgccgatc ccccgctccg
    17281 tgatctgctc gccgagccac atgatgagga tggtccccgc ggtctgcgtg atcatcgtca
    17341 tcagccggaa gcccatgccc gggtgcggca cgaccgaccc gccgcccatc gactcgagga
    17401 agcgggcgaa ggcgaacgac tgcgccgcgg ccagcaccac ggtgccgacg cgcgtgtact
    17461 gcgtgatctt cttccgcccc tcttcgcctt ccttctgcag cttctcgaag tacggcacca
    17521 ccgcgccgag gagctgcatg atgatcgaga ccgagatgta cggcatgatc ccgagcgcga
    17581 acaccgtcgc cttggcgaag gagccgccca cgaacaggtc gtagaggccg aacaggctgt
    17641 tcgcctggct ggcgaaggcc gccacgagcg cctcgctgtt cacgcccggg gtcgggatgt
    17701 gcccgccgaa ccggtagacg cagagcagcg cgatcgtgaa gaggaggcgg cgcttgagct
    17761 ccggaatccg gaagacgttc tgcgcctttt cgagcatcga caccgtctag gccctcacct
    17821 tgccctggct cttgttccct ttgttcccct tgctgccccg ggccgcccgt ccgctcgcgc
    17881 gggccgcctc gcggccgccc ttcgcctcgg cgcgcgccgg cttcttcacg ccgcgcggac
    17941 gcggcgcctt cttctcggga agcgtgacgc tgccgccggc ggcctcgatc ttctcccggg
    18001 ccgacccgct caccgcgtcc acggccaccg tgagcttgag cgacaggtcg cccgtgccca
    18061 ggatcttgac cggaaggtcg gagcggtcga tcagccgctt cgctttcagc gtctcgcggt
    18121 tcaccgtctc gcccgcggcg aagcggtcga ggctctccac gttcaccacc tggtgcggca
    18181 cgcggtggat gttcgtgaag ccgaccttgg gaaggcggcg ctggatcggc atctggccgc
    18241 cctcgaacca ccgcgccacg ttgccgctct tgcgcgcccg ctgtcccttg tgcccgcgtc
    18301 cagccgtgcc gcccaggccc gacgccgggc cgaggccccg ccgcttccgg cggtgcgtcg
    18361 cgcccttcgc gggcttcagg ttgccgatgc ggagcgtgcc tctcccatcg gctctggatg
    18421 tggccgccat caccgcccgc ctttcgcctg ggccgagccg tcgtgcggct cgacgcgaag
    18481 cagatagtcc accgccgcga tcatgcctcg gatctgcggg gtgtcgttgt gaatgaccgt
    18541 gtcgtgcgga tgccggagac ccaacgcggc cagcgtgcgc ttgtgggtct tcaggcaccg
    18601 gatcttgctc ttcacctgcg tgatcttaag ctgtgccatt gccgtccatg cccttgcgcc
    18661 gaatgcccaa cagctcgtcc accgtctttc cgcgcatccc ggccacctcg ccggcacggc
    18721 ggagcgactg cagtcccacg accgtcgccc gcaccacgtt gtgcgggttg cccgaaccca
    18781 gcgacttcgt gaggatgtcc tggatgccgg cggcctcgag cacggcgcgg atcgcgccgc
    18841 ccgcgatgac cccggttccc gccgtcgccg gcttcatcag cacccggccg gcgccgaagt
    18901 gtccgatcac ctcatgaggg atcgtcgtcc ggatcatcgg cacctcgaac atccgcttct
    18961 tcgccccctc gccggccttc cggatggcgt cggagacttc gttggccttc cccagtccga
    19021 cccccacctt ccccttgccg tcgcccacgg ccacgatggc gttgaagctg aaccgccgtc
    19081 cgcccttcac gaccttggcg acgcggttca cgtggatgac gcgctcgatg aactcggaat
    19141 cggtctgctg cgggcctcgg ccccgcattc cacctggatg ggccatgact ctcctagaag
    19201 tgctttagaa gttgaggccg ccctcgcggg cgccgtccgc gaccgctttg acgcggccgt
    19261 ggaaaaggta gccaccgcgg tcgaagcaga cgcgcaggat gcccttttcc ttggccatcg
    19321 ccgcgacgtg cttgccgacc gccttaccgg cgccgacctt gcccttggcg gacttcacca
    19381 gctccgccac cgcgccatcg cgcgaggagg ccgcgagcag cgtgcggccg ttcaggtcgt
    19441 ccaccagctg gacgtagatg tgattcaggc tgcggtagac ggtgaggcgc ggccgctcgg
    19501 ccgtgccggt caccttgcgc cggatccgga gatggcggcg cttccgccct tcgagtctcg
    19561 ctttgccctt catcgttccg ttccgctccc gtgcgccggc ttacgccgac ttcccgccgg
    19621 cagccttgcc cgccttgcga cggatgtact cgcccgcgta cttgatcccc ttgcccttgt
    19681 acggctccgg cggacggtag ccgcggatgt tcgccgcgac ctgccccacc aggcccttgt
    19741 cgaagccgct cacgtgcagc cgcgtcgggt tctccaccgt gatggagatg ccggccggcg
    19801 gctcgaactc gaccggatgg ctgaagccga tctgcagcgt cagcttcttt ccggtcatcg
    19861 cggcgcggta gccggtgccc gtgatctcga gcgtcttcgt gaagccctgc gtcaccccat
    19921 ggatcatgtt cgccaggatg gcgcgcgtgg tcccgtgcat cgcgagggcc tgcttgccgc
    19981 ccccgacctg ggccaccacg acttcctgcg gctccacggt cacgtccacg ccgtggccga
    20041 gcgcgaagag catctcgccc ttcggcccct tgaccttgat cgagcgcccg tcccgcgcca
    20101 cggtgacgcc ggaggggatc gggatcggtt tctttcctac gcgcgacggc atgacgggct
    20161 ccttaccaga cgcgacagag gtactcgccg ccgagcccct cttcgcgcgc ctcgagatcg
    20221 gtcatgatcc ccgacggggt cgagatgagc gccgtgccga gcccgccctg caccttggga
    20281 atctggtgac ggcggacgta cacgcggcgc cccggcttgg agacccgctc cagcccctgc
    20341 agcaccggcc gctccttcgc gtcgtacttg agatagatgc gaaggacgcc ctgccgcttg
    20401 tcgtccatcg tcttgtagtt gttgatgtac ttctcgcgca ggagcacccg cacgatttcg
    20461 gccttcacgt tcgaggccgg aacgtcgcac ttcttgtgct tcgccttgca ggcgttgcgg
    20521 atgctggtca gcagatccgc gatcggatcg gttacggaca taatctccct tcgtgcgccg
    20581 cggggtattc gccggggacc gggactacca gctcgccttc accacacccg gaatctggcc
    20641 gagcagcgcc agctcccgga agcagatccg gcagatgccg aagtcccgga ggaacgcccg
    20701 ggaacggccg caacggtggc agcggttgta tttccgcacc ttgaacttgg gctcccgctt
    20761 ccatttctcg acgagcgact tcttggccaa aacgactcct cctgacgcga gtgcgcctag
    20821 cgctgacgga agggcatgtt catgagccgc aacagctcct gcccctcttc gtcgttccgc
    20881 gccgacgtga cgaacgtgat atccatgccc cggaccttct cgacgcggtc gtagttgatc
    20941 tcggggaaga tgatctgttc cgtcacgccc agcgtgtagt tgccccggcc gtcgaaggag
    21001 cgcgccggga ccccgcggaa gtcgcggatt cgcggcagcg ccacgttcac cagccggtcg
    21061 tagaactcgt acatccgcgc gccgcgcagc gtgaccatgc agccgatcgg ctgcccctcg
    21121 cgcagcttga agttggcgat cgacttcttc gccttccgca ctgagggacg ctgcccggtg
    21181 atctgggcca gctcggccac cgcggcgtcg agcatcttga tgttcacgag ggcgtcgccc
    21241 acgcccatgt tcacgacgat cttccggagc cgcggcgcct gcatcgggtt cgcgaaggag
    21301 aagcgcttca tcagctcggg gagcaccgac ttctcgtagt gctccttcag gcgcgggacg
    21361 cgcgccgggg cctcggcttc gccgacggcc gcggcgggac cgcccgcttc cttgcccttc
    21421 ttctccttct tcttgccgcc gccgaccgcc tgcttggcgt cgccgccctt gcctttgccg
    21481 gcgccgtcgc ccttgggctt ggcttccttc ttcttctcgt ctttctcgtc ggccattact
    21541 gagctctcac aatcgtctcg ccgcagcggc cgcagaagcg ctcgcgcttc ccgtccgtgt
    21601 ccggcttgac cgtgacccgc accccctgct gacacttggg gcagaggtgc atgacgttcg
    21661 acacgtggat cggcgcctcg cgctcgagga tgccgccctg ccggccggtg ccccgcggct
    21721 tggtatggcg cttgatgaag ttcaccttct cgacgatgac gcggttcttg tcgcgcagca
    21781 ccttgaggat gcggcccgac ttgccccggt cgtcgcccga gatcacgacc acctggtcgt
    21841 tcctgcgaaa gttcggcatc ttggtcggcg ttttcatgtc agatcacctc aggggccagg
    21901 gagacgatct tcatgaactc ccgctccctg agctcccgcg ccacggggcc gaaaatgcgg
    21961 gtccccttgg gctctttctg gtcgtcgatc agcaccgcgg cgttctgatc gaagcggatg
    22021 taggacccgt ccttgcggcg gacctccttg cgcgtccgga tcagcacggc cttgtgcacg
    22081 tcacccttct tcacggccgc gcccgggagc gcgtcctgga tggagaccac gatgacgtcc
    22141 ccgagcatgc cgtagcgctt gcgagtgccg cccaggacct tcacgacccg cgcgacccgc
    22201 gcgcccgagt tgtccgatat ggcgatgatg ctccgtggct gtaccatggc tccttccggc
    22261 tcctcccgcc cctcagcgct cgacgcgctg cacgaaatcg acgagacgcc agcgcttgtc
    22321 cttgctcagc ggacgcgtct ccatgaagcg gatcacgtcg cccaccttgc actcgttctt
    22381 ctcgtcgtga accttgaacc ggctgcggcg ccggacgtac ttgccgtact cctcgtgctt
    22441 caccagccgc tcgatggtga cgaccaccgt cttctgcatc gaatcgctca cgacgcggcc
    22501 ggtcatgaac ttccgccgtc cccgttcggg gctcgcagcg ccttccatca cgacttcttc
    22561 gcctttctct tagcggccgg cttcgacttg gccttggcct tggacgcgct cggccgggac
    22621 ttcgcggccg ccttcttggg cttcgccttc gccgtcttct tcttcgcgcc cgcgcgcgcc
    22681 ttggggcgcg cggccggcgc ggcgctccgc gcctcgctcg gcgcagcgcc cgtggctgcc
    22741 accgtccccg ccgcatgccc cgccagcggg cggatgccgc tcttgtgctc ggcgaggatc
    22801 gtgtgcatcc gcgccacgtc gcgcttcttc tcgcgcagca gcagcggatt ctggagctgg
    22861 cgcatctggt tccggaaacg aaggtggaac agctcctcct tcagctcgcg gatcttcgtg
    22921 aggatctcgt cctccgtcat ctcccggatc ttctcggcct tcagatcgcg catctcagct
    22981 ccccgctccc gtctgccgct cgacgaactt cgtcttgatc ggcagcttgc tcgcgcccag
    23041 cttcatcgcc tcgcgggcca gccgctcctc gataccttcc agctcgaaga ggacccgtcc
    23101 cggcttcacg acggccaccc agaactcggg cgagcccttt ccctttccca ttcgggtctc
    23161 ggccggcttc accgtgaccg gcttgtccgg aaagatccgg atccacatct ttccgccgcg
    23221 cttgatgtag cgcgtgatgg cgacgcgcgc cgcctcgatc tggcggttgg tgatccacgc
    23281 ggcttccatg gcctggaggc cgaactcgcc gaacgccacg gttccgcccg tgtaggcctt
    23341 gcccttgcgg cggccccgca tctgcttccg gtgcttgacc cgctttggta cgagcatcga
    23401 tcgaccccgt tacgcggtcc gggccggagt cgccggggac gcttcgcccg gctccttccg
    23461 ttccaggatc tcgcccttga agatccacac cttcacccca accgcgccgt agatcgtccg
    23521 cgccgtcgag cgcgcgaagt cgatgtcggc gcgcagcgta tggagcggaa cccgcccgtc
    23581 gcggtagctc tcggtacggg ccatttccgc gccgcccagc cgtcccgaac agacgatgcg
    23641 gacgccgccg gcgcccgagc gcatggtcga ggcgatcgcc ttcttcatgg cgcggcggaa
    23701 ggagacgcgc tgctcgagct gccgggccac gtgctctccg accagggtcg cgtccatgtc
    23761 cggcttcttc acctcgtcga tgttcaggaa gatctccttc ttcatcgtga ggtgctggag
    23821 ctcgtcccga agctgatcca cctgctggcc gcgccgcccg atcaccagcc ccggacgcgc
    23881 cgtggagatg ttgatcgtca ccttggaggc ggtccgctgg atcgcgatcc gcgagatccc
    23941 cgcctgcgag aggcgcttct tcaggtagcg ccggatcacg atgtcctctt tcagcagcgc
    24001 gggatagtcc ttgcgcgcga accaccgcga atcccacgtg cggatgatcc cgagacgcat
    24061 cccgatcgga ttggttttct gacccacgag cttggccccc ttatgtccgg ccgccggagg
    24121 cggtcgaggt ctttgcggcg cggctcttac gagccggagc cttcttgcga gcaggagcct
    24181 tcgcaccctc ggtgaccgcc cctggagctg cttctgccgg tcgtgccgtc cgcgcgcgcc
    24241 gcgtcggcgc cggaacgtcc acgacgtcgc cttccaccac gaccaggatg tggcacgtgc
    24301 gcttcaagat cggcgtcgcg cgaccctgcg cgcgcgggag gaaccgcttc agcgtcggtc
    24361 ccgcctgggc caccgcttcc ttcacgtgca tgtgctcgat gttgatcttc ccttccatgg
    24421 ccacggcgtt cgccaccgcc gacttcatga ccttgccgat caccttggcc acgtgcttcg
    24481 gcgtgaagtc gaggatctcg gtcgcctgat ccacgcgctt cccgcgaatg agctgcagca
    24541 cctggtccgc cttccgcggc gtcacccgga tgtatcgcgc gatcgctttc gcctgcatgt
    24601 ccggtctcct gcccgcccgg ctacttcgcc ggagccggcg tcgccgcggc cttctcggac
    24661 gagccgtggc cgcggaagat ccgcgtcgcg gcgaactcgc ccagcttgtg gccgaccatg
    24721 ttttccgtga tgtacacggg gatgaacttg ttcccgttgt ggaccgccag cgtgtggccc
    24781 acgaactcgg gcgagatcgt ggagcggcgc gcccaggtct tgacgacgcg gcgctcgttc
    24841 gccttgttca tgccctcgat cttcttcaag agcttcgggt cgacgaaggg acccttcttg
    24901 atcgaacgac tcatgtccta tcgctcctct ggccttcggc ccgtcagctc ttcgctttcc
    24961 ggcggcgcag gatcagccgg tcggagagct tggtcttgcg cgtcttcttg cccttggcga
    25021 ggaggcccca gggcgagcag ggatgccgcc cgcccgagga cttgccttcg ccgccgccca
    25081 tggggtggtc gaccgggttc atcgccacgc cgcggacatt gggacggcgt cccatccagc
    25141 gggacttgcc cgccttgccg atcacgatgt tctgatggtc ctcgttcccc acctggccga
    25201 tcgtgcacca ggagcggagc ggaacccgcc gcatctcccc cgaggggagc cggagcgccg
    25261 cgtaatcgcc ttccttcgcc atcagctggc agaaggtgcc cgcgctgcgc gagagctggc
    25321 cgcccttgcc gggcaccagc tccacgttgt agatgaacgt gcccagcggc acgaagccca
    25381 gctccatcgc gttgcccggg cgcggctcgg cgtcgttccc cgagagcacc gtgtccccca
    25441 ccttgaggag ctgcggcgcc aggatgtagc gcttctcgcc gtccgcgtag tggagcagcg
    25501 cgatgcgcgc gctccggttc gggtcgtact cgatgtgcgc caccttggcc ggcacgccgc
    25561 gcttgtcgcg cttgaagtcg atcacccggt agagccgctt gtgcccgccc ccgcgcagcc
    25621 atgccgaggt gtggccggta ttggcgcggc cgcccttctt cggaaggtgc tgcgtcaggc
    25681 ccttctcggg accctgccgc gtgacgtcgt cgaacgaggc gaccgtgcgg aatcgctggg
    25741 tcggcgtgag cggcctgaat ttacggagcg ccatggctta tacctgctcg aagagatcga
    25801 tcgactcgcc cttcttgagc gtgacgacgg ccttcttcca atcgggtcga tgtcccgcgt
    25861 accggcccag cc
//
LOCUS       DASSLZ010000031        12527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_644550, whole genome shotgun sequence.
ACCESSION   DASSLZ010000031 DASSLZ010000000
VERSION     DASSLZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12527
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644550"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VFM00_01110"
     CDS             complement(<1..912)
                     /locus_tag="VFM00_01110"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HET7496873.1"
                     /translation="MIRRSFVSSLAALLLLLIAPQAARAAEPAAAKPAKGKPGAEQVR
                     FERLLERSTRERFAANPSLATYLGVHDFDTQLENTSKASVEAQAAAMRRALDELGTID
                     KAKLDADGRIDYDLFASDLKGRLFRATEVKEWEKDPGAYGYGWSIDNLIARNFAPPKT
                     RLRAVIARLRQVPRQFDNGRANVKNPAELLARFSAEDLEGLVAYLDNDVPHAFPTVKD
                     KALWKEYDAAKLEAQKATRAYVKWIREDLLPNAHGTYVLGEDAFRKRLLYEEMIDLPI
                     DSLLSIGMRELYRLEARYEDAAARVAPG"
     gene            complement(909..1709)
                     /locus_tag="VFM00_01115"
     CDS             complement(909..1709)
                     /locus_tag="VFM00_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7496874.1"
                     /translation="MIRHPLLLTASLGAAAGALGGMMGVGGGILLVPLLVHVMREPQH
                     EAQAVSLAFIIATAAVAVVPYLARERLDLGLGALLAAGALPGVVAGARTAGRLSALWL
                     RRAFGMAILLTAIRILVAPPVPAGTAMPWPWPAELLLGLGVGFLAGLLGIGGGTILVP
                     VLTLVQRMPQHAAQGLSLLLIVPVGLVGVATYARGGKLPTKLLPGLLAGGALGGLVGA
                     VLAQSIGGRSLARMFAVFLFVVSSQMIFSKPRPRTPAPGTQPISGGPS"
     gene            1757..3142
                     /locus_tag="VFM00_01120"
     CDS             1757..3142
                     /locus_tag="VFM00_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7496875.1"
                     /translation="MFFLERHRTRPRVYWTLGFIGALVLVINGTVFYSIRTSQAMLDV
                     ELGKRLEGTAQIAALLVRPEQIRALGAAVRDSAAAAQDSVTDFVVQMDAIEASDKVRA
                     AWSRLAEGAEASNVLLVDRSSHVLLRLKEPFGFERDVLTLDPASLATALIGEPSHSPL
                     YEKDRQYLRSGYAPVVDEDGQVLGAVVVEGGSRAFRPLEAIRTSLFGAALFASLLIVL
                     IWAGYQRTVGHLNRIEENLRHTDLLASVGQVAAGVAHEIRNPLAVLRGASQRLQKYEN
                     LNQAQRAELLAMIDEEVSRMGTVVQNFLDLSRRPKGETVDFELRPVLERSLDILRVEL
                     DRSKVSLEYEWDAPEMMVEGDPLAMHHVFLNLALNARDVMKDGGRLRVRVQGKKGEAR
                     IQFEDTGPGVPKAIRNKIFEPFYTTRATGTGLGLAFVDRILTEHGGSVTVGSAPSGGA
                     LFEIRLPAEAL"
     gene            3146..4543
                     /locus_tag="VFM00_01125"
     CDS             3146..4543
                     /locus_tag="VFM00_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7496876.1"
                     /translation="MAQRTLERILVAEDKENLCRLIEATLSEAGYEVAVALGGDAAIH
                     ELQARPFDLVVSDMRMPGASGAEVFRHARSLAGQPDVILMTAFGDAREAVQMMKEGAA
                     DYILKDNLLEELPVRVRQVLDHRKLRQESTVLRGRIESLQEQIHLHRFETIVGSSPAI
                     REALNLAQRVAPTDASVLLTGESGTGKEVYAQAIHAASHRCEAPFIPVNCGALPETLL
                     ESELFGHERGAFTGAIRTKPGRFEMADGGTVFLDEIGELPQSIQVKLLRFLQDHIYVR
                     VGGEEMRRADVRILAATNRNLEDMIHDGRFREDLYYRLNVFPIRLVPLRERREDIHPL
                     VNHFLIHQERTPDALASEALEFLVNYDYPGNIRELQNLIERACILAGKGPILRSHFPH
                     DRPRAAHDGADLLSMGLSLDQMERKMILEALERAQGNKTKAASLLGISRRALYSRMES
                     HAIPIGAAEPEPSEQ"
     gene            4932..5777
                     /locus_tag="VFM00_01130"
     CDS             4932..5777
                     /locus_tag="VFM00_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496877.1"
                     /translation="MFLPTPSSLSAGSTDELYGPFLPSASSFGPVPNTQPLSEGDRVA
                     REIVRTDAMIAGLEGRVRRSSSDKSRSDLRNARERQTEAREASGQSFYARANRLTLEA
                     RAYLKSALIQAGPPESDPEVVGRALEQTDDALARGKDIVEEASSSRQRLMLKSLQDRQ
                     ENARRLYKNGYVRSAYGETRDVRNGVVDLLRQCADLPVSRDTARRALRRAERVLVQTK
                     KEMGPRPASRALRLERDAELQLARARDSFTRNAFRDTLLHSKLVERHLQRAMEANRLA
                     TNQSD"
     gene            complement(5784..7466)
                     /locus_tag="VFM00_01135"
     CDS             complement(5784..7466)
                     /locus_tag="VFM00_01135"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7496878.1"
                     /translation="MPGEGQYLVVRRAGAEGERVSLDPGSAVTLGRSRENTVVLTDAS
                     VSREHARVFYREGSWWVEDQGSKNGTKVNGRRIEKASRLTPGDVLQLGNFQVAFSATG
                     SQVTARVADVAGPATLRSIKVEEFETGVDTRSFGAGLAPERVGAFLRAIDKVGQALLA
                     HRTVDELFAFVVELASDVLRADRTALLLRDPNSDALVAKAVKQAAGTEGDIVVSRSIA
                     RAAIELKQALLTGDAQSDTRFREQQSVIQQRIHSAMCAPLWHDDRVLGLIYVDNVAAP
                     APFEESDLRLLTLIAHLTAVKVRETEQYEAVERAGRLAEELRRAATMQQNLLPPEPVR
                     EGIVVVAGKNIPSFDVGGDYFDFIPAEDGCLVLGLGDVAGKGMAAAMLMMNLQASVRA
                     QVETGRPLADVMARLNRSIHHSVRGLRFVTLFVARIDTRSGAVNYVNGGHNPPYVLRA
                     ATGAMETLNAGGLLLGMFPEAEYEEGTIVLHPGDLLVAYSDGVTEARAPATAGEEFGE
                     EFGEERLVEFLQASRDLAAETLVEKLIARIQEFSGPAQLADDVTVAAIRLGS"
     gene            7520..8371
                     /locus_tag="VFM00_01140"
     CDS             7520..8371
                     /locus_tag="VFM00_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496879.1"
                     /translation="MARDIRTIRAAALEALGSFRFHEFAPFAWILAAELAVLILAMNL
                     GTAWGMASAGWLSSRLTGDRVLHYPIFYAFLPTLMTNVEAVLYAFAGSVLIPLALIRV
                     LRPMDPAAFPAASTGARLKQAFFTVLVAAVLCEALILGWQFVLNQPAVLKAIRAVLRS
                     SFASSIGVTVLGMLVGYALWTLFVYIPVSAVQPGRGFGAAFGGGLREGLANFFPTYLV
                     VIGLSLPAAAILLVLQVMGSFLVTQIRPEIIGVLIGLYAILSMLATFFIYSAAARFHQ
                     ARQAEAA"
     gene            8368..9891
                     /locus_tag="VFM00_01145"
     CDS             8368..9891
                     /locus_tag="VFM00_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7496880.1"
                     /translation="MRPRGTLRLLLVAILALTAGCGTAERRAERYRIEQAIYQAKKAE
                     TEAFLGRSRPDSVGLLRLRDGYLRVRDAAHPPFLKGSAKELAVGREALQLVAAAQSQA
                     ARFGMMAGRPDLALQSAEWLEQHAEGDPMTMRQADFVTAGALRAVGKGEEAIERMRGM
                     LRRYEPVPPPPGTSQEDEILGVPEMMAQVRREMGDQAGALRELDYGNGYYKGLLQKPR
                     EPMLEALIRARIVRNDLELNRAPEALEQVTALEKLVAAHPDLNALTPELRYSRAKIRL
                     MTNAADVQGIAMLEQFAKEYPRHSLAPRALFDAAVYLENAKQFPDALARYREVVALYP
                     RNLDVAPVSLFRQGMLEERTGAWDAAKATLESIPVKYPRTQAAVEAPFTIAMRYYARG
                     DREAAKAALARAIVVYQTMIANDSTSALVPVCKFNVLRGQLSLGQWDQALVTVDDLAS
                     HFPRHPYTAQALLEGAKVADANKQKDRAAGYLQQYLENFPTSPLAAQVRQEKDKLMR"
     gene            10137..10931
                     /locus_tag="VFM00_01150"
     CDS             10137..10931
                     /locus_tag="VFM00_01150"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7496881.1"
                     /translation="MIRKVLFLGAAGLLLGASAAFAFPPIDLTTAGATYTSATDGTIW
                     TQLVNQPTGTGVYNPFLRLRASGSEEGMNTDGDANSTYNDVAGIWTHSITLGQLNTVT
                     VGGVNYYSFSVDINEPNGGGQNLLSLDELRIYTLNGSASLMTEAAVTGAGGVLRYNLD
                     GTIDQDVYMDYNLAAGSGHDDVQVLIPTSFFAGASASDQIYFYSKFGDTAGMIADADF
                     TSGDGFEEWHALLGEAPPPPSLPEPSTVMILGTGLLGLAATRMRKK"
     gene            11094..>12527
                     /locus_tag="VFM00_01155"
     CDS             11094..>12527
                     /locus_tag="VFM00_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7496882.1"
                     /translation="MKEFQTSQIRNVALASQHGTGKTSLAEAFLWRTKVTTRHGRVED
                     GTTALDFTPEETHRKISIGLSVAPVEWRNHKVNLIDTPGYADFAGDLVAGLRAADGVI
                     LCLKAPAGVEAGTEAVWEKIEDRGCATLAVITQMEKEHANFAGALARASERLGKRFVA
                     VGWPIGQAEAFRGIVDLASHKAFFFEKDGSMKEGPVPAEMEAEISQARSALVDAAAEA
                     DDTLLEKFLGGEELSVEEIHKGLREGVCARSFVPAVPVSAYPMHGVDFVLDTIVDLMP
                     SPADCGPVVGHKPGSEEKVEFPPDAAGHLAAIVFKTSSESHAGDLSMIRVFSGTLAPG
                     KEVWNSSAQRAEKIGQLFTIVGKERKDAATIPAGDIGAAVKLRETHTGQTLCDKSHPI
                     VLAPVPFPGATNEVAIAPKHKGEEEKMGSGLHKLREEDPTIHVRVEGDLHQTVLSGLG
                     DLHIDVLLEKLTRRFGVHVETAKPRIPY"
BASE COUNT         1999 a         4401 c         4206 g         1921 t
ORIGIN      
        1 gccgggcgcg acgcgcgcgg cggcgtcctc gtagcgggcc tccaggcgat acagctcgcg
       61 catgccgatc gagagaaggg aatcgatcgg cagatcgatc atctcctcgt agaggagccg
      121 cttccggaac gcatcctccc cgaggacgta ggtgccgtgc gcgttgggga ggaggtcctc
      181 gcggatccac ttcacgtagg cccgcgtggc cttctgggcc tcgagcttgg ccgcgtcgta
      241 ttccttccag agggccttgt ccttcaccgt cgggaaggcg tgcggcacgt cgttgtccag
      301 ataggcgacc agcccctcca gatcctccgc cgagaaacgg gcgaggagct ccgcggggtt
      361 cttcacgttg gcgcggccgt tgtcgaactg ccgcggcacc tgccggaggc gcgcgatcac
      421 ggccctgagg cgcgtcttgg gaggagcgaa gttgcgcgcg atcaggttat cgatcgacca
      481 gccgtagccg taggcgcccg ggtccttctc ccattccttg acctcggtcg cgcgaaagag
      541 ccgccccttc agatcgctcg cgaagaggtc gtagtcgatc cgcccgtcgg cgtcgagctt
      601 cgccttgtcg atggtcccga gctcgtccag cgcgcggcgc atcgccgcgg cctgcgcctc
      661 gacggaggcc ttcgaggtgt tctcgagctg cgtgtcgaag tcgtgcacgc cgaggtacgt
      721 ggcgagcgag gggttggcgg cgaagcgctc ccgcgtcgac cgctccagca gccgctcgaa
      781 ccggacctgc tccgcgccgg gcttgccctt ggctggcttt gcggccgcgg gctcggcggc
      841 gcgcgccgcc tgcggcgcga tgaggagaag gagcagagcc gcgagagacg agacgaacga
      901 acggcggatc atgacgggcc tccggaaatc ggttgggtgc ccggggcggg cgtccgcggc
      961 cggggcttgc tgaagatcat ctgcgacgag acgacgaaca ggaacaccgc gaacatccgc
     1021 gcgagcgacc ggcctccgat gctctgcgcg agcaccgcgc cgaccagccc ccccagcgcc
     1081 ccgccggcga ggagccccgg aaggagcttc gtcgggagct ttcccccgcg cgcgtaggtg
     1141 gccacgccga ccagccccac gggcacgatc aggagaagcg agagcccctg cgcggcgtgc
     1201 tgcggcatcc gctgcaccag cgtgagaaca gggaccagga tcgtcccacc gccgatcccg
     1261 aggagcccgg ccaggaaccc gacgccaagc cccaataaga gctcggccgg ccagggccag
     1321 ggcatcgccg ttcccgccgg aaccggcggc gcgaccagga tccggatcgc ggtcaagaga
     1381 atggccattc cgaaggcgcg gcgcagccag agcgcgctca gccgcccggc ggtgcgcgcg
     1441 cccgccacca cgccgggcag cgctcccgcg gcgagcagcg cccccagccc caggtccagc
     1501 cgctcccgcg ccaggtacgg cacgaccgcg accgccgcgg tcgcgatgat gaacgccagc
     1561 gagaccgcct gcgcctcgtg ctgcggctcg cgcatcacat ggaccaggag gggcaccagg
     1621 aggatccctc cccccacgcc catcatcccg cccaaggcgc cggcggccgc gccgagcgaa
     1681 gccgtcagca acagcggatg ccgtatcatg acgttcggat ggctaaccta gcacaggccg
     1741 gccccaggct cggctgatgt tctttctcga gcggcaccgg acccgcccgc gcgtctactg
     1801 gacgctcggg ttcatcgggg cgctggtgct cgtcatcaac ggcaccgtct tctactccat
     1861 ccgcacctcc caggcgatgc tggacgtgga gctggggaag cgcctcgagg gcaccgccca
     1921 gatcgcggcc ctcctcgtcc ggcccgagca gatccgcgcg ctcggcgccg cggttcggga
     1981 ttcggccgcc gccgcgcagg actcggtcac cgatttcgtc gtccagatgg acgcgatcga
     2041 ggcgtcggac aaggtgcgcg ccgcgtggag ccgcctggcc gagggcgccg aggcgtcgaa
     2101 cgtcctcctg gtcgaccggt cctcccatgt gctcctgcgc ctcaaggagc cgttcggctt
     2161 cgagcgcgac gtgctgacgc tggaccccgc gtcgctcgcg accgcgctca tcggcgagcc
     2221 ctcccactcg ccgctctatg agaaggaccg ccagtacctc cgatcgggct acgccccggt
     2281 ggtggacgag gacggccagg tgctgggcgc cgtcgtggtg gagggaggct cgcgcgcgtt
     2341 ccggccgctc gaggcgatcc ggacgtccct gttcggggcc gccctgttcg cctcgctcct
     2401 catcgtcctg atctgggcgg ggtatcagcg gacggtggga cacctgaacc ggatcgagga
     2461 gaacctgcgc cacaccgacc tcctcgcttc cgtggggcag gtggccgcgg gggtcgcgca
     2521 cgagatccga aaccctctcg ccgtgctccg gggcgcctcc cagcgccttc agaagtacga
     2581 aaacctgaac caggcgcagc gggcggagtt attggcaatg atcgacgaag aggtgagccg
     2641 gatgggcacg gtggtccaga acttcctcga tctctcccgg cgaccgaagg gagagaccgt
     2701 ggatttcgag ctccggccgg tgctggagcg ctccctggac atccttcgcg tggagctgga
     2761 ccggtccaag gtatcgctgg aatacgagtg ggacgccccg gagatgatgg tcgagggaga
     2821 tcctctggcg atgcaccacg tctttctgaa cctagcgttg aacgcgcgcg acgtcatgaa
     2881 ggacggcgga cggctgcgcg tccgggtgca gggcaagaag ggggaagccc gtattcagtt
     2941 tgaagatacc ggtcccgggg ttcccaaggc gatccggaac aagatcttcg agccgttcta
     3001 cacgacccgg gcgacgggga ccggcctggg gctcgcgttc gtggatcgga ttctcacgga
     3061 gcacggcgga tcggttacag ttgggagcgc cccgtccggg ggcgccctgt tcgaaattcg
     3121 tcttccggcc gaggccctgt agctgatggc gcaacgcacc ctggaacgca tcctggtcgc
     3181 cgaggacaag gagaacctct gccgtctcat cgaggcgacc ctatccgaag ccggctacga
     3241 agtggcggtc gcgctcggag gcgacgccgc catccacgag ctccaggccc gccccttcga
     3301 tctcgtcgtc tccgatatgc ggatgccggg cgccagcggc gccgaagtct tccggcatgc
     3361 gcgctccctg gccggccagc ccgacgtcat cctcatgacc gcgttcggcg acgcgcgcga
     3421 ggcggtccag atgatgaagg agggggcggc cgactacatc ctgaaggaca acctcctgga
     3481 agagctgccg gtccgcgtgc gccaggtgct ggaccaccgg aagctccgcc aggaatccac
     3541 ggtgctgcgc ggccggatcg agagtctcca ggagcagatc catcttcacc gcttcgagac
     3601 gatcgtgggg tcgagccccg cgattcgcga ggcgctgaac ctggcgcagc gcgtggcgcc
     3661 cacggacgcg agcgtcctcc tcacggggga gagcggcacc gggaaggagg tctacgcgca
     3721 ggcgatccac gcggccagcc atcgctgcga ggctcccttc atccccgtga actgcggcgc
     3781 gctccccgag accctcctcg aatcggagct gttcggccac gagcggggcg ctttcaccgg
     3841 cgccatccgc accaagccgg gccgcttcga gatggccgac ggcggcacgg tcttcctgga
     3901 cgagatcggc gagctgcccc agagcatcca ggtcaagctg ctgcgcttcc tccaagatca
     3961 catctacgtt cgcgtcgggg gcgaagagat gcgccgcgcc gacgtgcgga tcctggccgc
     4021 gacgaaccgg aacctcgagg acatgatcca cgatgggcgc ttccgcgagg atctctacta
     4081 ccggctgaac gtctttccga tccgcctcgt cccgttgcgg gagcgtcgcg aggacatcca
     4141 cccgctcgtg aatcatttcc tgatccacca ggaacggacg cccgacgcgc tcgcctcgga
     4201 ggccctggaa ttcctggtga actacgacta ccccgggaac atccgcgagc ttcagaacct
     4261 gatcgagcgg gcctgtatcc tcgcggggaa ggggccgatc ctccgctccc atttcccgca
     4321 cgaccgcccg cgcgccgccc acgacggcgc cgacctgctc tcgatggggc tcagcctgga
     4381 tcagatggag cggaagatga tcctcgaagc cctcgagcgg gcacagggga acaagacgaa
     4441 agccgcttcg ctcctcggca tctcgcgccg cgcgctctac tcgaggatgg agagccacgc
     4501 catccccatc ggcgccgccg agcccgagcc gtcggaacag taataccggt cgcgcccatg
     4561 tccctgtcgc gcccagggcg cgacaggaac ccctaggccg tacgcacaca cccctcgcat
     4621 cacgttcaca ccgctcccgg accgttccgt acagaagaag gtaccttccc gtccaagagt
     4681 ccccgcagcg cgcccccact tcccgcgttc gaggttgcga accctcccgg taactccatc
     4741 ctgtaaacgg ggttttgcac ccgttgcgga ttcggacgtg gtaaccctat gcatggcgtg
     4801 ccgtttgcat cgtttcttcg cgccatgccg gaacgaacga tggatgccga ccgaacagct
     4861 cagccctgac gcgaggaccc atgcgccgcg accatgcccg gatactgcct gctcttctgg
     4921 ctgccgcggc attgttcctt ccgacgccct cgtccctctc ggcgggtagc accgacgagc
     4981 tgtacggtcc ctttctcccc tccgcctctt cgttcggacc tgtccccaac acccagcccc
     5041 tctccgaggg tgaccgcgtc gcgcgcgaga tcgtccgcac ggacgccatg atcgccggcc
     5101 tcgagggccg cgtgcgccgg agctccagcg acaagtcgcg ctcggacctg cgcaacgcgc
     5161 gggagcgtca gaccgaggca cgcgaggcga gcgggcagag cttctacgcg cgtgccaacc
     5221 ggctcaccct ggaggcgcgc gcctatctga agagcgccct catccaggcg ggcccgcccg
     5281 agagcgaccc cgaggtcgtg gggcgcgccc tcgaacagac cgacgacgcg ctggcccggg
     5341 ggaaggacat cgtcgaagag gcctcctcct cgcgccagcg gctcatgctg aagagccttc
     5401 aagatcgaca ggagaacgcc cggcggctgt acaagaacgg ctacgtgcgg agcgcctacg
     5461 gggagacgcg cgacgtgcgg aacggcgtgg tcgatctgct ccgccagtgc gcggatctgc
     5521 cggtctcgcg cgacaccgcg cggcgcgcgc tgcgccgggc cgagcgggtg ctggtccaga
     5581 cgaagaagga gatggggccg aggccggcgt cacgggcgct gcgcctggag cgcgacgccg
     5641 agctgcagct cgcgcgggcg cgcgattcct tcacgaggaa cgcgttccgc gacacgctgc
     5701 tccattccaa gctggtcgag cgccatctcc agcgggcgat ggaagccaac cggctcgcga
     5761 ccaaccagag cgattgaggg gagctacgag cccagccgga tcgccgccac cgtcacgtcg
     5821 tcggcgagct gggccgggcc gctgaattcc tggatgcgcg cgatcagctt ctcgaccagg
     5881 gtctcggcgg ccaggtcgcg cgaggcctga aggaactcga ccagccgctc ctccccgaac
     5941 tcctcgccga actcctcgcc cgccgtcgcg ggagcccgcg cctccgtcac gccgtcgctg
     6001 taggcgacca gcagatctcc cggatggagc acgatcgtcc cctcctcgta ctcggcctcg
     6061 gggaacatgc cgagcaggag ccctcccgcg ttcagggtct ccatcgctcc cgtggcggcg
     6121 cgcaggacgt agggcgggtt atggccgccg ttcacgtagt tcacggcgcc gctccgggtg
     6181 tcgatccgcg ccacgaagag cgtgacgaat cggagcccgc ggacgctgtg gtggatggag
     6241 cggttcagac gcgccatcac atcggccagg ggccgccccg tctcgacctg cgcgcggacc
     6301 gaggcctgga ggttcatcat cagcatcgcg gccgccatcc ccttgccggc cacgtcgccc
     6361 agtcccagaa ccaggcatcc gtcctcggcg ggaatgaagt cgaagtagtc gccgccgacg
     6421 tcgaaggagg ggatgttctt cccggccacc acgacgatcc cctcgcggac cggctcgggt
     6481 gggagcagat tctgctgcat ggtcgcggcg cggcggagct cctcggccag ccgtcccgcg
     6541 cgctccaccg cctcgtactg ctccgtctcg cgcaccttga ccgcggtgag atgcgcgatc
     6601 agcgtgagca gccggaggtc gctctcctcg aacggcgccg gggcggcgac gttgtcgacg
     6661 tagatgagcc cgagcacccg gtcgtcgtgc cagagcgggg cgcacatcgc cgagtggatg
     6721 cgctgctgga tcacgctctg ctgctcccgg aaccgggtgt cgctctgcgc gtcgcccgtg
     6781 aggagcgcct gcttcagctc gatcgcggcg cgcgcgatgc tgcggctgac gacgatgtcc
     6841 ccctccgtcc cggccgcctg cttcaccgcc ttggcgacca gcgcgtcgga attcggatcc
     6901 cgcaggagga gggcggtgcg gtcggcgcgc agcacgtcgc tcgccagctc gacgacgaac
     6961 gcgaacagct cgtccaccgt gcgatgggcc agcagcgcct ggcccacctt gtcgatggcg
     7021 cgcaggaacg cgcccacgcg ctcgggcgcg agccccgccc caaacgaacg ggtgtccacc
     7081 ccggtctcga actcctcgac cttgatcgag cgcagggtgg cgggcccggc cacgtcggcg
     7141 acgcgcgccg tgacctggct cccggtcgcc gagaaggcca cctggaagtt gcccagctgg
     7201 agcacgtcgc cgggcgtgag ccgcgaggcc ttctcgatgc ggcggccgtt caccttcgtc
     7261 ccgttcttgc tcccctggtc ctccacccac caggagccct cccggtagaa gacgcgcgcg
     7321 tgctcgcgcg agaccgaggc gtccgtcagg acgaccgtgt tctcgcggga gcgcccgagc
     7381 gtgacggccg agcccgggtc gagcgaaacg cgctcgcctt cggcaccggc ccgccggacg
     7441 acaaggtact ggccctctcc aggcatgcgc ggtagtctcc agcgttcacc cctcggaatc
     7501 aacacggatc gggaggaata tggcgcgcga catccgcacg atacgcgccg cggcgctgga
     7561 ggcgctcggc agtttccgtt tccacgaatt cgctcccttc gcctggattc tcgcggcgga
     7621 gctggccgtt ctgatcctgg cgatgaacct gggcacggcg tggggcatgg cctccgcggg
     7681 atggctctcc agccgtctca ccggcgaccg cgtgctgcac tacccgattt tctacgcgtt
     7741 tctaccgacg ctgatgacca acgtcgaggc cgtgctctac gcgttcgcgg ggagcgttct
     7801 gatcccgctc gccctgatcc gcgtcctgcg tcccatggat ccggcggcct tcccggcggc
     7861 gtcgaccggg gcgcggctga agcaggcgtt cttcaccgtg ctggtcgcgg ccgtgctctg
     7921 cgaggcgctg atcctgggat ggcagttcgt cctcaatcag ccggcggtgc tgaaggccat
     7981 ccgggccgtc ctccgcagct cattcgccag ctcgatcggc gtcaccgtgc tgggcatgct
     8041 ggtcggctac gccctctgga ccctcttcgt ctacatcccc gtgtcggccg tgcagccggg
     8101 acggggcttc ggggccgcgt tcggcggagg tcttcgcgag gggctggcga acttcttccc
     8161 gacctacctc gtcgtgatcg gcctctcgct ccccgccgcg gcgatcctgc tcgtcctgca
     8221 ggtgatgggc tcgttcctcg tgacccagat ccgccccgag atcatcggcg tgctgatcgg
     8281 cctgtacgcg atcctcagca tgctcgcgac cttcttcatc tacagcgcgg cggcgcgctt
     8341 ccaccaggcc cgccaggcgg aggcggcatg agaccgcgcg gaacgctccg cctgctcctc
     8401 gtcgcgatcc tcgcgctcac cgccgggtgc ggcacggccg aacgacgcgc cgagcgctat
     8461 cgcatcgagc aggcgatcta ccaggcgaag aaggcggaga cggaggcctt cctcgggagg
     8521 agccgccccg actcggtcgg gctgcttcgg ctgcgggacg gatacctccg cgtccgcgac
     8581 gcggctcatc cgcctttcct caaaggttcg gcgaaggagc tggcggtcgg gcgcgaagcg
     8641 ctccagctgg tcgccgcggc gcagtcgcag gccgcgcgct tcgggatgat ggcgggccgc
     8701 cccgacctcg cgctccagag cgcggagtgg ctggagcagc acgccgaggg agatccgatg
     8761 acgatgcgcc aggccgactt cgtcaccgcg ggcgcgctcc gcgccgtggg caaaggggaa
     8821 gaggcgatcg agcggatgcg cggcatgctt cgccggtacg agccggtgcc gccgccgccc
     8881 gggacctcgc aggaggacga gatcctcggc gttccggaga tgatggcgca ggtgcgccgc
     8941 gagatggggg accaagccgg cgcgctgcgg gagctggact acggcaacgg ctactacaag
     9001 ggcctcctcc agaagccgcg cgagccgatg ctcgaggcgt tgatccgggc gcgcatcgtc
     9061 cggaacgacc tggagctgaa ccgcgcgccc gaggcgttgg agcaggtgac cgcgctcgag
     9121 aagctggtcg ccgcgcatcc cgatctgaac gcgctgacgc ccgagctgcg ctattcgcgc
     9181 gcgaagatcc gcctgatgac caacgccgcg gacgtccagg ggatcgccat gctcgagcag
     9241 ttcgcgaagg agtatccgcg gcactcgctc gcgccgcgcg cgctcttcga cgcggcggtc
     9301 tacctcgaga acgcgaagca attccccgac gcgctcgcgc gctatcgcga agtggtcgcg
     9361 ctctacccgc gcaatctcga cgtcgcgccc gtgtcgctct tccgccaggg catgctcgag
     9421 gagcggaccg gcgcgtggga cgcggcgaaa gcgaccctcg aatcgatccc ggtgaagtat
     9481 ccgcgcacgc aggccgccgt cgaggcgccc ttcaccatcg cgatgcgcta ctacgcgcga
     9541 ggcgaccgcg aggcggccaa ggccgcgctg gcccgggcga tcgtggtcta ccagacgatg
     9601 atcgccaacg actcgacgtc cgcgctggtc ccggtctgca agttcaacgt cctgaggggt
     9661 cagctctccc tggggcagtg ggaccaggcg ctcgtcaccg tggacgatct ggcgtcccac
     9721 ttcccgcgtc atccctatac ggcacaagcg cttctggagg gggccaaggt ggcggacgcg
     9781 aacaagcaga aggaccgggc ggccgggtat ctccagcagt atctcgagaa cttcccgacc
     9841 tcgccgctgg ccgcccaggt caggcaggaa aaggacaaat tgatgcgcta gacaggcagg
     9901 aagcgcgggg gtatcgtttc gacagcgcga ttcgcaccgc aatccgctcc cgccctgaaa
     9961 tttgcagttg attgcgagtc gcattcctgc tacactttct gtgttcaggt gtaagcaggt
    10021 ttttccagcg tcgctttccc ggcctggggg ggttgttccg ggacggcggc gttaagtccg
    10081 gtggcgtggg ccttcatccc cgactggctt gcgcattgca tctagggggt taccgaatga
    10141 tccgtaaggt gctcttcctc ggcgccgcag ggcttctcct cggcgcgtcg gcggcatttg
    10201 ctttcccgcc gatcgacctc accacggcgg gcgcgaccta cacgagcgcg accgacggca
    10261 cgatctggac ccagctggtc aaccagccca cgggtacggg cgtgtacaac ccgttcctcc
    10321 gccttcgcgc ctctggctcg gaggagggga tgaacacgga cggcgacgcc aacagcacgt
    10381 acaacgacgt cgccggcatc tggacccaca gcatcacgct cggtcagttg aacacggtca
    10441 ccgtgggtgg ggtgaactac tactccttct cggtggacat caacgagccg aacggcggtg
    10501 gccagaacct gctgtcgctg gacgagctgc gcatctacac cctgaacggc agcgcgtcgc
    10561 tgatgacgga agctgcggtg acgggcgctg gtggcgtgct ccgctacaac ctggacggca
    10621 ccatcgacca ggacgtgtac atggactaca acctggcggc cggcagcggt catgacgacg
    10681 tccaggtgct gatcccgacg agcttcttcg ccggcgcgtc ggcctcggac cagatctact
    10741 tctactcgaa gttcggcgat acggccggca tgatcgccga cgccgacttc acctcgggcg
    10801 atggtttcga agagtggcat gccctcctgg gcgaggcccc tcctccgccg tcgcttcccg
    10861 agcccagcac ggtcatgatc cttgggaccg gactcctcgg tctggccgcg acccggatgc
    10921 gcaagaagta agctcgaaag caaagtcggt actccccggc gaggcggtgg ttttcaccgc
    10981 ctcgccgctt tcttttcccc cctcgagtgt ggtagcattt ccgaacaagc tatctacagc
    11041 aaccgcttat aattaccagc ggttccggag cgaccggaac caggggggct cccttgaaag
    11101 agttccaaac cagccagatc cgtaacgtcg cactcgcctc gcagcacggc accggaaaaa
    11161 cctccctcgc tgaagcgttc ctctggcgga ccaaggtcac gacgcgtcat ggccgggtcg
    11221 aagacggcac caccgcgctc gacttcacgc cggaggagac ccatcgcaag atctcgatcg
    11281 gcctgagcgt cgcgccggtc gagtggcgca atcacaaggt caacctcatc gacacgccgg
    11341 gctacgccga cttcgcgggc gacctcgtcg cgggtctgcg cgcggccgac ggcgtgatcc
    11401 tctgcctcaa ggctcccgcg ggcgtcgagg cgggcaccga ggcggtgtgg gaaaagatcg
    11461 aggaccgcgg atgcgcgacg ctggcggtca tcacgcagat ggagaaggaa cacgcgaatt
    11521 tcgcgggcgc gctcgcgcgc gcgagcgagc ggctggggaa gcgcttcgtc gcggtgggat
    11581 ggccgatcgg ccaggcggag gcgttccgcg gcatcgtgga tctcgcctcg cacaaggcgt
    11641 tcttcttcga gaaggacggc tcgatgaaag agggccccgt ccccgcggag atggaggcgg
    11701 agatcagcca ggcccgctcc gccttggtgg acgccgcggc cgaagccgac gacacgctgc
    11761 tcgagaagtt tctcggcggc gaagagctct ccgtcgagga gatccacaag gggctccgcg
    11821 agggggtgtg cgcgcgctcg ttcgtgccgg cggttcccgt ctcggcgtat ccgatgcacg
    11881 gagtcgattt cgtgctcgac accatcgtcg acctgatgcc ctcgcccgcc gactgcgggc
    11941 ccgtggtggg ccacaagccg ggcagcgagg agaaggtgga gtttccgccc gacgcggccg
    12001 ggcatctggc cgcgatcgtc ttcaagacga gcagcgagag ccacgccggc gacctctcca
    12061 tgatccgcgt cttctcggga acgctcgccc cgggcaagga agtgtggaat tccagcgcgc
    12121 agcgcgcgga gaagatcggg cagctcttca ccatcgtggg caaggagcgg aaggacgcgg
    12181 ccaccatccc cgccggcgac atcggggccg ccgtgaagct gcgcgagacg cacaccggcc
    12241 agaccctgtg cgacaagagc catcccatcg tgctcgcgcc cgttccgttc cccggcgcga
    12301 ccaacgaggt cgccatcgcg cccaagcaca agggcgaaga ggagaagatg ggctcgggcc
    12361 tccacaagct gcgcgaggag gatccgacca tccacgtccg cgtcgagggc gatctgcacc
    12421 agacggtcct ctccggtctg ggcgatctcc acatcgacgt gctcctcgag aagctgacgc
    12481 gccgcttcgg cgtgcacgtc gagaccgcca agccgcgaat cccctac
//
LOCUS       DASSLZ010000032         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1026634, whole genome shotgun sequence.
ACCESSION   DASSLZ010000032 DASSLZ010000000
VERSION     DASSLZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026634"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFM00_01160"
     CDS             complement(<1..461)
                     /locus_tag="VFM00_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HET7496883.1"
                     /translation="MSGRPRPAGFLSILLHAHLPFVRHPEYEDSLEERWLVEALVECY
                     LPLLRMLEGRAGRGARGRVTISLSPTLLAMLADPLLRSRMAFRMGRLLELAERDRARY
                     PGTSPFGPVAAHYAERFRAGIAQYERYGRNLAAAFRAVEEAGVARLITCGAT"
     gene            complement(458..1027)
                     /locus_tag="VFM00_01165"
     CDS             complement(458..1027)
                     /locus_tag="VFM00_01165"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HET7496884.1"
                     /translation="MSSLERLAPRESAVSTEAPPAAWRGRRHGEDRLHLLVRDPHRVF
                     AAWELTEALARRAGAIASSRGAPVRYALQIERRGEADGAPRAATRAALPDALGGEGWY
                     VDLARGGGSCRAVLGLDLPGGDFEPLLASRWMPVPPDGPCRETGDWPVDEVRRAWLER
                     EAERQRGRAVTPLPSSASRYLASPQAPKP"
     gene            complement(1050..>2478)
                     /locus_tag="VFM00_01170"
     CDS             complement(1050..>2478)
                     /locus_tag="VFM00_01170"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7496885.1"
                     /translation="EFMIGRVAVPILFAESDGTVDPNHFDWTPALRDSVLSSAVRGFL
                     KWSVMAASRNIPLTFLIEPHFGLATRYEPSDRPIGQETLWIEDVLEPLLGTKGDALTM
                     AYSYANAARARLNAQWAAIAFAVQNDTSATGTFPDGYIAHSELGGPWFVMPVNNLNTK
                     SATLDYYFEHEITHQFWALDEYPALNAWWACTLTTGYFNRPNRNASVPADGYCGIPTV
                     HCLMKGNYPDDWCEFTTDQIGWVDLDGTGVLDLYETRPVVRPDSTNYRTGAGLAITLR
                     GEADETAFPNRNPYRSGAGDSISIATVDSIEYRLDNGSWVPIPCGDGRCDSGEERFTL
                     VLPPLSTGSHIVEWRAWNSSGRTALVPSGTTLNISGASPESPGGGGAVPPAAPRVVAA
                     PAPLRGEARLRLTGPPGAVGSASILDLAGRVVRTWPVAVPGDGVLAWRWDGRGEGGGD
                     AAAGLYFVVVKIGTAVATHRVLLLR"
BASE COUNT          367 a          875 c          916 g          320 t
ORIGIN      
        1 gtggcgccgc aggtgatcag gcgcgccacg ccggcctctt cgacggcgcg gaacgcggcg
       61 gccaggttcc tgccgtagcg ctcgtactgc gcgatcccgg cgcggaagcg ttcggcgtag
      121 tgggccgcca ccgggccgaa gggagacgtc ccgggatagc gggcgcggtc gcgctcggcc
      181 agctcgagca ggcgccccat gcgaaacgcc atgcgcgatc gaaggagcgg gtcggcgagc
      241 atcgcgagca gggtcggcga gagagagatc gtgacccgcc cgcgcgcgcc gcggccggcg
      301 cgcccctcga gcatgcgaag gagcggcagg tagcactcga cgagcgcttc gaccagccag
      361 cgctcctcga ggctgtcttc gtattccgga tggcgcacga acggcagatg cgcgtggagg
      421 aggatcgaga ggaatcccgc gggacgcggg cggccgctca cggcttgggt gcctggggcg
      481 aggcgagata ccgggaggcg gaggacggaa gcggcgtcac ggcgcgaccg cgctgccgct
      541 cggcttcgcg ctcgagccac gcccggcgca cctcgtcgac gggccagtcg ccggtctcgc
      601 ggcagggccc gtcgggcggc accggcatcc agcgcgaagc caggagcggc tcgaagtctc
      661 cgcccgggag gtcgagcccg agcaccgcgc ggcaggaccc gcccccgcgc gccagatcga
      721 cgtaccagcc ctcgccgccg agcgcgtccg ggagggccgc gcgcgtggcg gcgcgcggcg
      781 ccccgtccgc ctcgcctctt cgctcgatct ggagcgcgta gcggaccggc gccccccggg
      841 acgaggcgat cgcgcccgcg cggcgcgcga gcgcctcggt cagctcccag gcggcgaaca
      901 cgcgatgcgg gtcgcgaacc aggagatgaa ggcggtcttc gccatgccgg cgcccccgcc
      961 aggctgccgg cggggcctcg gtcgagacgg cgctctcgcg cggggcgaga cgctcgaggg
     1021 agctcacggc cgctccggac gcggcgggat catcggagaa ggaggacgcg gtgcgtcgcg
     1081 acggcggttc cgatcttcac gaccacgaaa tatagccctg ccgccgcgtc gccgccacct
     1141 tccccgcgcc cgtcccagcg ccaggcgagc acgccgtcgc ccggcaccgc caccggccag
     1201 gtccggacga cccgtcccgc gaggtccagg atcgaggccg agcccacggc accgggcggc
     1261 cccgtgagcc gaagccgcgc ctcgccgcga agcggcgccg gtgccgcgac gacccggggc
     1321 gcggccggcg gaaccgcgcc gcctccgccg gggctttcgg gagaggcgcc ggagatgttc
     1381 agggtggtgc cgctcgggac cagggccgta cggccgctcg aattccaggc gcgccactcc
     1441 acgatgtggc tcccggtcga gagcggcggc agcacgagcg tgaaccgctc ctccccggag
     1501 tcgcaccgcc cgtcgccgca gggaatcggg acccacgacc cgttgtcgag gcggtactcg
     1561 atcgaatcca cggtggcgat cgaaatcgaa tcgcccgctc cggagcggta cgggttccgg
     1621 ttcggaaacg cggtctcgtc cgcctcgccg cggagcgtga tcgcgaggcc cgcccccgtg
     1681 cggtagttcg tcgaatcggg gcggaccacc ggacgcgttt cgtagaggtc caggaccccg
     1741 gtcccgtcca ggtccaccca gccgatctga tcggtggtga actcgcacca gtcgtccggg
     1801 tagtttccct tcatcaggca gtgcaccgtc ggaatgccgc agtagccgtc cgcgggcacc
     1861 gaggcgttcc ggttcggccg gttgaagtag ccggtggtca gcgtgcaggc ccaccacgcg
     1921 ttcagcgcgg gatactcgtc gagggcccag aactggtgcg tgatctcgtg ctcgaagtag
     1981 tagtcgaggg tggcgctctt ggtgttcagg ttgttcacgg gcatcacgaa ccagggtccg
     2041 ccgagctccg aatgggcgat gtagccgtcc gggaaggttc cggtcgccga cgtgtcgttc
     2101 tggacggcga aagcgatcgc ggcccactgg gcgttcaacc gcgcgcgcgc cgcgttggcg
     2161 taggaatagg ccatcgtgag cgcatcccct ttcgtgccga gcaacggctc gagcacgtcc
     2221 tcgatccaga gcgtctcctg gccgatcggc cggtcgctcg gctcgtaccg ggtcgcgagc
     2281 ccgaagtgcg gctcgatcag gaacgtgagc gggatgttcc gcgacgcagc catcacgctc
     2341 cacttgagga agccgcgcac cgccgagctc aggaccgagt cgcgaagcgc gggcgtccaa
     2401 tcgaagtgat tcggatccac ggtcccgtcg ctctcggcga agaggatcgg aacggcgacg
     2461 cgcccgatca tgaattcg
//
LOCUS       DASSLZ010000033         9817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1152945, whole genome shotgun sequence.
ACCESSION   DASSLZ010000033 DASSLZ010000000
VERSION     DASSLZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152945"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VFM00_01175"
     CDS             complement(<1..493)
                     /locus_tag="VFM00_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7496886.1"
                     /translation="MDGYPDHRSLYRLPWSLPDNPIAWLEPTEACNLYCEGCYRANQY
                     QGHKSLRDIESDLDTFARYRSFDGVSIAGGDPLTHPEVVSIVALARAKGYKPILNTNG
                     LALSDELLRELKAAGLVGFTFHIDSKQARPGWKGKTETELNELRQEFAERVARVGGIS
                     VAFN"
     gene            complement(546..2054)
                     /locus_tag="VFM00_01180"
     CDS             complement(546..2054)
                     /locus_tag="VFM00_01180"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET7496887.1"
                     /translation="MACAAHPALAQSDSATAKPPAAATPPAAASTPTAPKPAVDQCYA
                     CHSEQETPAATTYKNDVHRTVGVTCAGCHGGDPTTDDQDAAMSKAKGFVGVPKKDQIP
                     AMCGKCHGPGENAYKSRYKLDDAFGDFKESVHGHALAGNPKGPQCISCHGVHEIARVA
                     DARSPVHPTHVVETCAKCHSDAAYMRDFSPTLPVDQKEKYLTSVHGKRHAMGDPKVAT
                     CVSCHSNHRIFQAKDPRANVYPTNVPKTCAHCHGDAKYMAGYGIPTNQYSDYVQSVHG
                     KALLVKTDLNAPACNSCHGNHGAAPPGVSSVIAVCGRCHQMNQELYERSAHRAVFEGK
                     KLPGCVVCHGNHNVQPASDRIVSFEPQSPCARCHVNTPADKAAPDIRRLHLVLDSLST
                     GQTAALAVLDRAENLGMDVSDARYSLKDVNQSAVQARVSIHSFKVKEVEEAARPGIAI
                     IAQARTAGQEAIHEYGFRRQGLAVSTLIVTFLVIMLWLKIRDIEKKQKEKGR"
     gene            complement(2153..3244)
                     /locus_tag="VFM00_01185"
     CDS             complement(2153..3244)
                     /locus_tag="VFM00_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HET7496888.1"
                     /translation="MTHTEGGKRGGGIRGWIEERFNLAPAIEFASHKQVPEWKGSFWY
                     YFGGVSLFLFIVQVATGILLLMYYQPGDKTAFESVRFIVTKVKFGWLIRSIHSWAANL
                     FILSVFIHMFSVYFTKAFRRPRELTWYSGFILLGLAFAFGFSGYLLPWNTLAYFATKV
                     GTTIVAVLPGLGEPIMELLRAGKDVSTATLTRFYGLHVAIFPALFTVILGVHLMLIQT
                     QGMHEPESWDQVPQGKRRYMPFFPNFVLRDLLLWLIVLNVLAILAVWFPWELGKKAEP
                     FAAAPGGIKPEWYFLFMYQALKYFPAKLAGLDGEVVGIILFGIAGLLWFLVPLWDSHT
                     PGGMRNRVNTYVGILVVLFMIIFTALGVK"
     gene            complement(3254..3700)
                     /locus_tag="VFM00_01190"
     CDS             complement(3254..3700)
                     /locus_tag="VFM00_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET7496889.1"
                     /translation="MDQPSKPGISRRSFLDKLLGLGALAWFGSVIYPIFEYLKTPPQG
                     EAEVTSVVAAKVKDLKPNTGTVFRFGREPAILVSGPDGKLRALSATCTHLQCTVQYRP
                     DMQRIWCACHNGVYDLTGRNVSGPPPRPLTEYVVTLKGDDVVVTRA"
     gene            complement(3865..6186)
                     /locus_tag="VFM00_01195"
     CDS             complement(3865..6186)
                     /locus_tag="VFM00_01195"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HET7496890.1"
                     /translation="MPAYVIAILLICALIPWLSVVLFAVFWRDIRALKARMDRLEQGL
                     GAAPVARAPVAPPAAAAPAAPPLPVTPQAAAPAIAPSGPIVPSMPIAPALETPHHTTP
                     APPARTGLEEQIGGIWMQNVGSVLLLLGAFFLIVWGYANGRLGPEVLVLAGVALGIVV
                     SWRGHVIGRTLTALGNAFIGVGLGVIYITLYLGHFRMHVFPGAVAFTLLAATSLITVA
                     IGLRRREPIIAILGVIAAYLPQLLAVWIPLQGFKLALPALLGYFAVVNVVVFALAASV
                     GWSGLVLLSLVLTTVTWSANAGLHAWSLGIQLALSGLFVALGLAPVARLVRHERPVRS
                     IDLAVVASAPILLLLASWPFVVAPDRLRAALLLSALAALYAVVSIWLERRRDERDLWR
                     PLTATATVFLAAGIERGLLPEYLALAWCAEGAALLWLGMGPRGAWLRLLGDTVLTVAA
                     IRLLWVLLTGASPAVPSLLSAPTLRDLLVIGVFLLASHLIGRRRDLLAPEERLAPGGW
                     VFVANALLMIWAVREMGQVRHFLPQVSGSDNDAIAVTLAGCAWMIQCFALFQLSIARS
                     APVLRQVGYFVGGFAVLALFTGHAARDPWIAGSLPLLNTSALIVAFATTVLILGAELL
                     WRHRDRLSPGERRIPEVATAVANLVLLAWWAREAGHLASALTPSDAAFGARGPAVRTL
                     AAVFTSAAWTLQAVILFALGWLRGSAFLRWSGLVLFGLTVLKFLLVDLDRVDAFWRFV
                     SALGIGAALLAVSFLYQRKARSAGSLRGSPEAR"
     gene            6547..8787
                     /locus_tag="VFM00_01200"
     CDS             6547..8787
                     /locus_tag="VFM00_01200"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET7496891.1"
                     /translation="MSLEIRLHGRGGQGGVTCAKILAATYARLGKSVQTFGDYAGERS
                     GAPVRAYTRVSDEPIQNRNKIYEPDHVLVLDPTLLDKDAVAGLARGGILLVNTSEAPA
                     AVEARFGPFRAATIDATAIARRRGIGTRSVVIVNTTIAGAYARAADIPFEALERAYKD
                     LHLDRDLGAAREAYDAVALNGHAGAEGAPAPAAAKQKPAETAPLEATAGQPVLPLTEH
                     VEGPATGLKTGSWRTQVPRYVEQLPPCNAWCPAGNDVVGFIQAVAKDGEAVAAAILAK
                     SQPFASVCGRVCPAPCMEVCNRRPYDGAVNIRGLERWIGDHATAVRDDVPTRRDRRSV
                     AVVGSGPAGMTAACELARAGHVVRIYEGEASLGGVLRTGIPAYRLPREALDREIESIL
                     GLGIETRCGEFLDARAITRLRRQHDAVILAAGLGRPTELDVLGADLPGVEQGTKFLHR
                     VNLEGGASLHGHVMVLGGGNTAIDCARSAIRSGASMVTVAYRRTRTEMPAIKEEIAEA
                     EREGISFLYQRQPVAFHGRRRLTQVELAEVQMGEPDASGRRRPVVSARTARFECDYAL
                     LALGQGADPGLLPEGAKIQENRVHYDGGPTNLFVAGDFATNDGTVAHAIGDGRRAAAR
                     VLAALGEPATFPVPPDRPRAVTSEMIRFEHFPVTAGSHTIEIPLAGREASFEETNLGL
                     SDPREAGRCFSCGRCTQCDTCMAYCPEGIIERRAGGYDVQLDFCKGCGICVTECPRHA
                     MEMAPV"
     gene            8784..>9817
                     /locus_tag="VFM00_01205"
     CDS             8784..>9817
                     /locus_tag="VFM00_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET7496892.1"
                     /translation="MSLQLISANHASAAAVTLAARANRKARGFCSGVYPITPQTECIE
                     KLCGEEIERGAIVRVESEHSAMGVCIGASLSGARSFTASSSNGLAYMAENVFAAGFYR
                     LPIVMMAVNRTLGPPWNIWVDHGDTLMLRDAGWIQFYCEDNQEVHDTLLLAFRLAEHE
                     RVLLPVMVCQDAFVLSHTMMMTEIASQEDVDAFLPELSLPHRIADRPRVVGGLDFPHQ
                     TEEHRKQHLEAMRRVPGVYATVQDEFEKRFGRRPADTVVPYRTEDADVLVVSMGTTAS
                     TVRAAVDRLRDEGIRAGALRLRMFRPLPEALLREYLGGKKRVAVLDRDTCPGLGGIVW
                     SEVRGLVDPGA"
BASE COUNT         1584 a         3272 c         3519 g         1442 t
ORIGIN      
        1 cgttgaacgc cacggagatc ccgcccaccc gggcgacgcg ctcggcgaat tcctggcgca
       61 gctcgttcag ctccgtctcg gtctttcctt tccagcccgg acgcgcctgc ttgctgtcga
      121 tgtggaacgt gaacccgacc aggcccgcgg ccttcagctc gcgcaggagc tcgtcgctga
      181 gggcgaggcc gttggtgttc aggatcggct tgtagccctt cgcgcgcgcc agcgccacga
      241 tcgagaccac ctcggggtgg gtgagcgggt cgccgccggc gatcgatacg ccgtcgaagg
      301 agcggtagcg ggcgaaggtg tcgaggtcgg actcgatgtc ccggaggctc ttgtgcccct
      361 ggtactggtt cgcgcggtag cacccctcgc aatacagatt gcacgcctcg gtgggctcga
      421 gccaggcgat ggggttgtcg gggaggctcc aggggagccg gtagaggctg cggtgatcgg
      481 gatagccgtc catggattcc tccggggacc cgtcgcggtc gcggaggacg ggtcggcgtg
      541 gggcgctagc gccctttctc cttctgcttc ttttcgatgt cgcggatctt cagccagagc
      601 atgatgacga ggaaggtgac gatgagcgtg gaaacggcca ggccctggcg gcggaatccg
      661 tactcgtgga tcgcctcctg acccgccgtg cgcgcctggg cgatgatcgc gatgccgggg
      721 cgtgccgctt cctccacctc tttcaccttg aagctgtgga tcgagacgcg ggcctgcacc
      781 gcggactgat tcacgtcctt cagcgaatag cgcgcgtccg acacgtccat gccgaggttt
      841 tccgcgcggt cgaggaccgc gagcgcggcg gtctgcccgg tggagaggct gtccagcacc
      901 agatgcagcc gccggatgtc gggcgccgcc ttgtccgcgg gcgtgttcac gtggcacctg
      961 gcgcagggcg actgcggctc gaacgagacg atccggtcgc tcgccggctg cacgttgtga
     1021 ttgccgtggc acacgacaca ccccggcagc ttcttcccct cgaagaccgc gcggtgcgcg
     1081 ctccgctcgt acagctcctg gttcatctgg tggcagcgcc cgcagacggc gatcaccgat
     1141 gagacgcccg gcggggcggc gccatgattg ccgtggcagg aattgcaggc cggcgcgttc
     1201 aggtcggtct tcacgagcag cgccttgccg tggacgctct ggacgtagtc ggaatactga
     1261 ttcgtgggaa tgccgtaccc ggccatgtac ttcgcgtcgc cgtggcagtg ggcgcaggtc
     1321 ttcgggacgt tcgtcggata gacgttcgcg cgggggtcct tggcctggaa gatccgatgg
     1381 ttcgagtggc agctcacgca cgtcgcgacc ttgggatcgc ccatggcgtg gcgcttcccg
     1441 tgcacgctgg tgaggtactt ctccttctgg tccaccggca gggtggggga gaagtcgcgc
     1501 atgtaggcgg cgtcgctgtg gcacttggcg cacgtttcga ccacgtgcgt cgggtgcacg
     1561 ggggagcgcg cgtcggcgac gcgggcgatc tcatggacgc cgtggcagga gatgcactgg
     1621 ggtcccttgg gattgcccgc aagcgcatgc ccgtggacgc tctccttgaa gtccccgaag
     1681 gcgtcgtcca gcttgtagcg gctcttgtac gcgttttcgc ccggtccgtg gcacttcccg
     1741 cacatcgccg ggatctgatc cttcttggga acgccgacga accccttggc cttgctcatc
     1801 gcggcgtcct ggtcgtcggt cgtgggatcg ccgccgtggc atccggcgca ggtcacgccg
     1861 accgtgcgat ggacgtcgtt cttgtaggtg gtcgctgctg gggtctcctg ctcggagtgg
     1921 caggcgtagc actggtcgac ggcgggtttg ggcgctgtgg gggtgctcgc ggctgcaggc
     1981 ggcgtcgccg cggcgggggg cttcgcggtg gcggagtcgg attgagccag ggctgggtgc
     2041 gcagcgcacg ccatcgcgag agcgagggtg aatgcgaggg cgatcgccgc tgttccgctg
     2101 cttcgcagcg gtacattgct tcgcagcggg acatggcttc gccacgccaa cattatttca
     2161 cccccagcgc ggtgaagatg atcatgaaca gcaccaccag gatgcccacg taggtgttca
     2221 ctcggttccg catcccgccc ggggtgtgcg agtcccagag cgggaccaaa aaccagagca
     2281 ggcccgcgat tccgaagagg atgatcccca cgacctcgcc gtcgagcccc gcgagcttcg
     2341 cggggaagta cttcagcgcc tggtacatga agaggaagta ccactccggc ttgatccctc
     2401 ccggcgccgc ggcgaagggc tcggccttct tgcccagctc ccacgggaac cacaccgcca
     2461 ggatggccag cacgttgaga acgatcagcc agaggagcag gtcgcggagc acgaagttgg
     2521 ggaagaacgg catatagcgc cgcttcccct ggggcacctg gtcccacgac tcgggctcgt
     2581 gcatcccctg cgtctggatc agcatcaggt gcacgccgag gatcaccgtg aacagggcgg
     2641 ggaagatcgc cacgtggagg ccatagaagc gggtgagcgt cgcggtcgag acgtccttgc
     2701 ccgcgcgcag gagctccatg atcggctcgc ccagccccgg cagcaccgcc acgatggtcg
     2761 tgcccacctt cgtggcgaag taggccagcg tgttccaggg gagcaggtag ccactgaatc
     2821 cgaacgcgaa cgccaggccc agaaggatga acccggagta ccaggtcagc tcgcgcggcc
     2881 ggcggaaggc tttcgtgaaa tagaccgaga acatgtggat gaagacggag agaatgaaga
     2941 ggttggccgc ccaggagtgg atcgatcgga tcagccaccc gaacttcact ttcgtgacga
     3001 tgaaccggac gctctcgaag gccgtcttgt cgcccggctg gtagtacatg agaaggagga
     3061 ttccggtggc gacctgcacg atgaagagga agaggctgac gccgccgaag tagtaccaga
     3121 aggagccctt ccactcgggg acctgcttgt gggaggcgaa ctcgatcgcg ggggcgaggt
     3181 tgaatcgctc ctcgatccag ccccggatcc ctccgccccg cttcccgccc tccgtgtggg
     3241 tcacgcgtcg gtcctaggca cgggtcacga ccacatcgtc gcccttcagc gtcacgacgt
     3301 actcggtgag cggacggggc ggcgggcccg agacgttgcg ccccgtgagg tcgtagaccc
     3361 cgttgtggca ggcgcaccag atacgctgca tgtcggggcg gtactgcacc gtgcactgga
     3421 gatgggtgca ggtggcggac agggcgcgca gcttgccgtc cggcccgctt acgaggatgg
     3481 cgggctcgcg tccgaagcgg aagaccgtcc ccgtgttcgg cttcagatcc ttcaccttcg
     3541 cggcgacgac gctcgtcacc tcggcttcgc cctgcggggg cgtcttcagg tactcgaaga
     3601 tgggatagat gaccgagccg aaccacgcga gggcgccgag accgaggagt ttgtcgagga
     3661 aggagcggcg tgagattcca ggcttcgacg gctgatccat gcagttgcct ccgctgccag
     3721 gtcgccgatc cgaacaatgg gacgggatcg cggggaagat ggcacggtcc cgttcccgca
     3781 gcaagagaat gcgtggggcc gtgccgacgg tccaggtagc caatggtggt aggcggccgc
     3841 tgcgtcctgc gcggccgccg tcgactacct cgcctccgga ctgccgcgca gcgatcccgc
     3901 cgaacgcgcc tttcgctggt acaggaacga cacggcgagg agtgccgcgc cgattccgag
     3961 cgcgctcacg aaccgccaga acgcgtccac gcggtcgaga tccaccagca ggaacttgag
     4021 cacggtgagc ccgaaaagga cgaggccgct ccatcggagg aacgccgatc cgcggagcca
     4081 tcccagcgcg aacaggatca cggcctgcag cgtccaggcg gcgctggtga agaccgcggc
     4141 cagcgtgcgc acggcaggcc ctctggcgcc gaacgccgcg tcggacggag tcaaggccga
     4201 agcgaggtgg cccgcttcgc gcgcccacca ggccagcagg accaggttcg ccaccgcggt
     4261 cgccacttcg gggatccggc gctcgccagg ggagagccgg tcgcggtgcc gccagagaag
     4321 ctcggccccc aggatcagaa cggtcgtggc gaaggcgacg atcagcgccg acgtgttgag
     4381 gagcggcaga gatccggcga tccacggatc gcgagcggca tggccggtga agagcgcgag
     4441 cacggcgaag ccgccgacga agtagccgac ctgccggaga accggcgccg agcgggcgat
     4501 cgagagctgg aacagcgcga agcactggat catccaggcg catcccgcca gcgtcacggc
     4561 gatcgcgtcg ttgtccgagc cggagacctg gggcaggaaa tgccggacct gccccatctc
     4621 gcgcaccgcc cagatcatga ggagcgcgtt ggccacgaag acccaccctc ccggcgcgag
     4681 ccgctcctcg ggcgcgagaa ggtcgcggcg ccgcccgatg agatgcgacg cgagaaggaa
     4741 cacgccgatc acgagcagat cgcgcagcgt cggggcgctg agcaggctcg gtacggcggg
     4801 cgaggccccg gtgagaagca cccagaggag ccgtatcgcc gcgaccgtga ggaccgtatc
     4861 gcccagcagg cggagccacg ctccacgggg tcccatgccc agccagagga gtgctgcccc
     4921 ctcggcgcac caggccagcg ccaggtattc ggggaggagt ccgcgctcga tgcccgccgc
     4981 gaggaaaacg gtcgcggtcg cggtcagggg gcgccagagg tcgcgctcgt cgcgccggcg
     5041 ctccagccag atcgagacca cggcatagag cgcggccagc gccgagagaa gcagtgccgc
     5101 gcggaggcgg tcgggcgcga ccacaaacgg ccaggaggcg agcaacagga gaatcggcgc
     5161 cgacgcgact accgcgaggt cgatcgatcg aaccggtctc tcgtggcgga cgagccgcgc
     5221 gacgggggcg aggccgagcg cgacgaagag gccggagagc gcgagctgga tgcccaggct
     5281 ccaggcgtgg agtcccgcgt tggcgctcca cgtgaccgtg gtcagcacga gcgacaggag
     5341 cacgaggccg ctccatccga cggaggccgc gagcgcgaag acgacgacgt tgacgacggc
     5401 gaagtagccg aggagggcgg ggagtgcgag cttgaacccc tggagcggaa tccatacggc
     5461 gagcaactgg gggaggtagg ccgcgatcac gcccaggatg gcgatgatcg gctcccgccg
     5521 gcggagcccg atcgccaccg tgatcagcga ggtggccgcg agcagcgtga aggcgaccgc
     5581 gcctgggaag acgtgcatgc ggaaatggcc caggtagagc gtgatgtaga tcacgccgag
     5641 accgacgccg atgaacgcgt tgcccagggc ggtcagggtg cgtccgatga cgtgcccgcg
     5701 ccacgagacc acgatcccca gcgccacgcc ggccaggacc aggacctccg gtccgaggcg
     5761 cccgttggcg tagccccaca cgatcaggaa gaacgcgccc aggaggagca ggaccgagcc
     5821 cacgttctgc atccagatgc cgccgatctg ctcttccagt cctgttcgcg ccggaggcgc
     5881 gggtgtggtg tggtggggcg tttcgagagc cggcgcgatc ggcatcgagg gcacgatcgg
     5941 acccgagggc gcgatcgcgg gagcggcggc ttgcggcgtg acagggagtg gcggagcagc
     6001 gggcgcagcg gcagcgggtg gcgccacagg ggctcgcgcg acaggggccg caccgagacc
     6061 ttgctccagg cggtccatgc gcgccttgag cgcacggatg tcgcgccaga agacggcgaa
     6121 gaggacgacc gaaagccagg gaatcagcgc gcagatcaga aggatcgcga tcacgtacgc
     6181 gggcatgccg ctctcctccg atcgtgccgc gggaaaacgg gtgtgggtcg agtggctccc
     6241 taccttaggg gagatgcgcg cggcgcgcca gcctctcgtc cgatctgccg cggtcgcacg
     6301 tggctcgctt tcggaactcg acatctcggg ccgcatcctc gcccacgatc agtctcgtcc
     6361 cgcgaccaac gcccgtcgat gcgaccttgg gcgtatctac aaaagggttc gcatcgagga
     6421 cggcgcgccg agcgcgctgc ccttctacct tcggctacct atcttcccta cccccggact
     6481 cctacgctcg gttctccggc ggtccgatag ttggacgtgg cccaccggac ggaggaaagg
     6541 ggcggcatgt ccttggagat tcgcctacac ggtcgcggcg gtcagggcgg cgtgacgtgc
     6601 gccaagatcc tggcggccac ctatgcccgt ctcggaaaga gcgttcagac cttcggcgac
     6661 tacgccggcg agcgctcggg agcccccgtc cgcgcctaca cccgcgtctc cgacgagccg
     6721 atccagaacc gcaacaagat ctacgaaccc gaccacgtcc tcgtcctcga tccgacgctc
     6781 ctcgacaagg acgccgtcgc cgggctggcc cggggcggga tccttctcgt gaacacgtcc
     6841 gaggctcctg ccgccgtcga agcccggttc ggcccgttcc gcgccgccac catcgacgcg
     6901 accgcgatcg cgcgccgccg ggggatcggc acgcgttcgg tcgtcatcgt gaacaccacg
     6961 atcgcgggag cttatgcccg cgccgcggac atccccttcg aggcgctgga gcgggcgtac
     7021 aaggacctcc acctggatcg cgacctgggt gccgcgcgcg aggcctacga cgccgtcgct
     7081 ctgaacggac acgccggcgc cgagggagcg ccggctccgg ctgcggcgaa gcagaagccc
     7141 gccgagaccg cgccgctcga ggcgaccgcg ggccagccgg tcctcccgct cacggagcac
     7201 gtcgaagggc cggccaccgg tctcaagacc ggctcgtggc ggacccaggt tccgcgctac
     7261 gtggagcagc ttccgccgtg caacgcgtgg tgcccggcgg gaaacgacgt cgtgggattc
     7321 atccaggcgg tggccaagga cggagaggcc gtggcggcgg ccattctcgc gaagtcgcaa
     7381 cccttcgcgt cggtgtgcgg ccgcgtctgc ccggccccct gcatggaggt gtgcaaccgc
     7441 cgtccctatg acggcgcggt gaacatccgc ggcctggagc ggtggatcgg agatcacgcg
     7501 acggcggtgc gggacgacgt gcccactcgc cgtgaccgcc ggagcgtggc cgtcgtggga
     7561 agcggccccg ccggcatgac cgccgcctgc gagctggcgc gcgcgggaca cgtcgtccgc
     7621 atctacgagg gggaggcgtc gctgggtggc gttcttcgca ccggcatccc cgcctaccgg
     7681 cttccgcgcg aggcgctgga ccgggagatc gaatcgatcc tgggcctggg catcgagacg
     7741 cgctgcggcg agttcctcga cgcgcgcgcg atcacgcggc tccgccggca gcacgacgcg
     7801 gtgatcctcg cggcggggct gggccggccc acggagctgg acgtcctggg cgccgatctt
     7861 cccggcgtgg agcagggaac gaagttcctc catcgcgtga atctcgaggg gggtgcttcg
     7921 ctccacggcc acgtcatggt gctcgggggc gggaacaccg cgatcgactg tgcccggagc
     7981 gcgatccgct ccggcgcctc gatggtgacg gtggcgtacc gccgcacccg taccgagatg
     8041 cccgcgatca aggaggagat cgccgaggcc gagcgcgagg ggatctcctt cctctaccag
     8101 cgtcagccgg tcgccttcca tgggcgccgc cgcctgacgc aggtggagct ggccgaggtg
     8161 cagatgggcg agcccgacgc gagcggccgc cgccgtcccg tggtcagcgc ccgcacggcg
     8221 cggttcgaat gcgactacgc gctgctcgcc ctggggcagg gggccgatcc gggcctcttg
     8281 cccgagggcg cgaagatcca ggagaaccgc gtgcactacg acggaggccc cacgaatctc
     8341 ttcgtcgcgg gcgacttcgc cacgaacgat gggaccgtgg cccacgcgat cggcgacggc
     8401 cgccgcgccg cggcgcgcgt gctcgcggcc ctcggcgagc cggcgacctt cccggtgccg
     8461 cccgaccgtc cacgcgcggt gacgagcgag atgatccgct tcgagcactt cccggtgacg
     8521 gccgggtcgc acaccatcga gatcccgctc gccggccgcg aggcatcctt cgaggagacc
     8581 aacctcggcc tctccgatcc gcgcgaggcc ggacgctgct tctcctgcgg gcgctgcacg
     8641 caatgcgaca cctgcatggc gtactgcccc gaagggatca tcgagcggcg cgccggcggc
     8701 tacgacgtgc agctcgactt ctgcaagggg tgcgggatct gcgtcaccga atgcccgcgc
     8761 cacgccatgg agatggcccc ggtatgagcc tccagctgat cagcgccaac catgcttcgg
     8821 cggcggccgt cacgctggcc gcccgcgcca accgcaaggc gcgcgggttc tgcagcggcg
     8881 tctatccgat cacgccccag accgagtgca tcgagaagct gtgcggcgag gagatcgagc
     8941 gcggcgcgat cgtgcgcgtg gagagcgagc acagcgcgat gggcgtctgc atcggcgcct
     9001 cgctctccgg cgcgcgctcg ttcaccgcct cctcgtcgaa cggcctcgcc tacatggcgg
     9061 agaacgtctt cgcggccgga ttctaccggc ttccgatcgt catgatggcg gtgaaccgga
     9121 cgctgggccc cccctggaac atctgggtgg atcacggcga cacgctgatg ctgcgcgacg
     9181 cgggatggat ccagttctac tgcgaggaca atcaggaggt gcacgacacc ctcctcctcg
     9241 cgttccggtt ggccgagcac gagcgggtgc tcctgccggt catggtctgc caggacgcct
     9301 tcgtcctctc gcacacgatg atgatgacgg agatcgcgag ccaggaggac gtggacgcct
     9361 tccttcccga gctgtcgctg ccgcatcgaa tcgccgaccg gccgcgcgtg gtgggcgggc
     9421 tggacttccc gcaccagacc gaagagcacc ggaagcagca cctggaggcg atgcgccgcg
     9481 tgcccggcgt ctacgccacg gtccaggacg agttcgagaa gcggttcggc cgccgtcccg
     9541 ccgatacggt ggtgccgtac cggaccgagg acgccgacgt gctggtggtc tcgatgggga
     9601 cgaccgcgag cacggttcgc gcggcggtgg accgcctgcg cgacgagggg atccgcgcgg
     9661 gcgcgctccg cctccgcatg ttccggccgc ttcccgaagc gctcctccgc gagtatctcg
     9721 gagggaagaa gcgcgtggcc gtgctcgacc gcgacacctg tccggggctg ggcgggatcg
     9781 tctggagcga ggtccggggc ctcgtcgatc cgggcgc
//
LOCUS       DASSLZ010000034         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_773805, whole genome shotgun sequence.
ACCESSION   DASSLZ010000034 DASSLZ010000000
VERSION     DASSLZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773805"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFM00_01210"
     CDS             complement(<1..492)
                     /locus_tag="VFM00_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7496893.1"
                     /translation="MSGRLEGRVILITGGARGIGGAACALAAREGAAVAVADILDAEG
                     EALAAAIRSRGGRALYVRLDVSREEAWAEAMERTARELGGLHALVNNAAIARDGDLEQ
                     ETLEGWNKVLSVNLTGSWLGMKAAVPLLRRSGGGAIVNVSSIYGAIGGNGAAAAYHAT
                     KGAL"
     gene            complement(489..1565)
                     /gene="cydB"
                     /locus_tag="VFM00_01215"
     CDS             complement(489..1565)
                     /gene="cydB"
                     /locus_tag="VFM00_01215"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET7496894.1"
                     /translation="MTTLWFWLVAAMIVGYVLLDGFDLGAGAVHFFVAKTPAERRAVL
                     STIGPVWDGNEVWLLAAGGTLYFAFPVLYATAFSGFYLPLMIVLWLLILRGLSIEFRN
                     HIESPAWTPLWDVLFSFSSILLAVVFGAALGNVVRGVPIDASGRFFEPLWTDFRPGPV
                     AGILDWYTVVVGLASLAALTMHGALWVALKTHDPIAARARHVASRVWWGVLVLTGLIT
                     MITMRLQPQVPANLARWPLGMLFPLLALLGLIGVPWFLARRNDGRAFLASVAYLAGML
                     TSVVFGLYPYVLPASTGMERALTIHNASAGETGLRIGVIWWTIGMILALIYHVFIYRH
                     FAGRVAVGASDGHGDYGQAGGETS"
     gene            complement(1567..>2203)
                     /locus_tag="VFM00_01220"
     CDS             complement(1567..>2203)
                     /locus_tag="VFM00_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET7496895.1"
                     /translation="QMVAKHQPTTLAAMEGLFNTEKGAPLAIIGQPDLERQTLDNPIL
                     IPRLLSFLTYKRWMHEIRGLNAFPKEDWPDNIPLLYYSYRIMVGLGTFFIAILCGAAF
                     LWARGALYRSRWMLWILMLAVPFPYIANTAGWITAEVGRQPWLAYGLYRTAEGYSRNV
                     SSGNGLFTLIGFMGMYTVLAILFLFLVRREVDHGPSSGPMLTQVPPAPRTL"
BASE COUNT          392 a          755 c          772 g          284 t
ORIGIN      
        1 gagcgcgccc ttggtcgcgt gatacgccgc cgcggcgccg ttgccgccga tcgccccgta
       61 gatcgaggag acgttcacga tcgcgccgcc gccggagcgg cgcaggagcg gaaccgccgc
      121 tttcataccg agccaggatc cggtgaggtt cacgctcagg accttgttcc atccctccag
      181 cgtctcctgc tccaggtccc cgtcccgcgc gatcgccgcg ttgttcacca gggcgtgcag
      241 cccgcccagc tcgcgcgcgg tgcgctccat cgcctccgcc caggcctcct cgcgcgaaac
      301 gtcgagccgc acgtagagcg cgcgtccgcc ccgagagcgg atcgcggccg cgagcgcctc
      361 cccttcggcg tccaggatgt cggccaccgc cacggccgcg ccctctcgcg ccgcgagcgc
      421 gcacgcggcg ccgccgatgc cacgcgcccc gccggtgatc aggatgacgc gaccctccag
      481 gcgaccgctc acgaggtctc tcctccggcc tgtccgtagt cgccatgacc gtcggacgcg
      541 ccgaccgcga cccgtccggc gaagtggcga tagatgaaga catggtagat cagcgccagg
      601 atcatgccga tcgtccacca gatcaccccg atgcgaaggc ccgtctcgcc cgcggaagcg
      661 ttgtggatcg tgagcgcgcg ctccatcccg gtgctcgcgg ggaggacgta gggatagagc
      721 ccgaagacca cgctcgtgag catgccggcg agataggcga ccgaggcgag gaacgcgcgg
      781 ccgtcgttcc ggcgcgccag gaaccagggc acgccgatca gcccgagcag cgcgaggagc
      841 ggaaagagca tcccgagcgg ccatcgcgcg agattcgcgg gcacctgggg ctggagccgc
      901 atcgtgatca tcgtgatcag ccccgtcagg acaagcacgc cccaccagac gcgcgacgcg
      961 acgtggcggg cccgcgcggc gatcggatcg tgggtcttga gcgcgaccca caaggcgccg
     1021 tgcatcgtga gggcggccag cgacgcgagc ccgaccacca ccgtgtacca gtcgaggatt
     1081 ccggcgaccg ggccggggcg gaagtcggtc cagagcggct cgaagaaccg gcccgaggcg
     1141 tcgatcggca cgccgcgcac gacgttcccg agcgcggcgc cgaagaccac ggccaggagg
     1201 atgctggaga aggaaaagag cacgtcccag agcggcgtcc aggccggact ctcgatgtgg
     1261 tttcggaact cgatcgacag gccgcgcagg atcaagagcc agaggacgat catgagcggc
     1321 aggtagaagc ccgaaaaagc ggtggcgtag agcaccggaa acgcgaagta gagcgtcccg
     1381 cccgcggcca ggagccagac ctcgttcccg tcccacaccg gcccgatcgt gctcagcacg
     1441 gcgcggcgct cggcgggggt cttcgccacg aagaagtgga ccgctccggc gccgaggtcg
     1501 aatccgtcca ggagcacgta gcccacgatc atcgccgcga cgagccagaa ccagagggtg
     1561 gtcatgtcag agggtccgcg gcgccggcgg cacctgggtc agcatcggtc ccgacgacgg
     1621 gccgtgatcg acctcccggc gcacgaggaa gaggaagagg atggcgagca ccgtgtacat
     1681 ccccatgaag ccgatcaggg tgaagaggcc gttccccgac gagacgttcc gcgaatagcc
     1741 ctcggccgtg cgatagaggc cgtacgccag ccagggctgg cgccccacct cggcggtgat
     1801 ccagcccgcc gtgttggcga tgtaggggaa cgggaccgcc agcatcagga tccagagcat
     1861 ccagcggctc cggtagagcg cgccgcgcgc ccacaggaat gcggcgccgc agaggatggc
     1921 gatgaagaag gtgccgagcc cgaccatgat ccggtacgag tagtagagga gcgggatgtt
     1981 gtccggccag tcctccttcg gaaaggcgtt caggccgcgg atctcgtgca tccagcgctt
     2041 gtaggtgagg aagctcagaa ggcgcgggat caggatcgga ttgtccagcg tctgacgctc
     2101 caggtcgggc tgtccgatga tcgcgagcgg cgcccccttc tcggtgttga agagcccctc
     2161 catggcggcg agggtggtgg gctggtgttt cgccaccatc tgc
//
LOCUS       DASSLZ010000035        15703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_900545, whole genome shotgun sequence.
ACCESSION   DASSLZ010000035 DASSLZ010000000
VERSION     DASSLZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 15703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15703
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900545"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFM00_01225"
     CDS             <1..592
                     /locus_tag="VFM00_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7496896.1"
                     /translation="GAHGPGAVLVDVRALGAIGADWYAANLHKWAFAPRSCGILWAAP
                     ERQEELHPPVISWGIGRGFLAEFDWVGTRDPSAWLATPAALDWFEELGAEAVREHNRA
                     LARRARSLLTERWGTEPAAPEAMTGPMTTIPMPDRLGSEPEDAARVRDRLLFEDRIEV
                     QVHAWRGRIWARVSAQLYNDDDDLERLAAAVAALHS"
     gene            687..1112
                     /locus_tag="VFM00_01230"
     CDS             687..1112
                     /locus_tag="VFM00_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007737578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET7496897.1"
                     /translation="MPARNSWFTRFAKATSRSTGRPIAFALAAAIIVLWAMTGPVFHF
                     SDTWQLVINTGTTIITFLMVFLIQNTQYRDSEAVHLKLDELIRAQEGAHNALLDLEDL
                     DDKELDRIRENYAQLADDARKILRRRGVDTGTPDVKNGR"
     gene            1202..2758
                     /locus_tag="VFM00_01235"
     CDS             1202..2758
                     /locus_tag="VFM00_01235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7496898.1"
                     /translation="MKSDSGATRSVWMDVPPLPARPPLDRDLRADVCVVGAGMAGLTI
                     AYLLAREGLRVAVLDDGPTAGGETSRTTAHLTNVIDDRYHWIEQVHGPEGARLAAESH
                     TAAIDTIEAIAEREQIACDLVRLDAYLFAGPGDPRDELERELEAARRAGLTGVAWVDR
                     APIEGFATGPCLRFPNQAQFHPLRYLQGVADAIERSGGRIHGGTHVTAVHHGPPPRVE
                     TESGVAVVADAIVVATNSPVHEMIGVHPKQAAYRTYVVAARVPRGVVTRALYYDTENP
                     YHYVRLQPESAGGPDLLIVGGEDHKTGQADDADERWAALEAWTRERFPLAREFVARWS
                     GQVMETMDGVAFIGPDRAGSRVFIATGDSGMGMTHSTIAGLLLRDLIAGRENPWAHLY
                     DPGRVSLRAAGELAKENLNFVSKYADYVTGGDVGSADEIPPGEGAVLRRGLTKVAAYR
                     DPSGTLHEMSAVCTHLGCVVRWNSAEKSWDCPCHGSRFDSHGRVVNGPATRDLAEKDT
                     AQPAAAWERA"
     gene            complement(2811..4196)
                     /locus_tag="VFM00_01240"
     CDS             complement(2811..4196)
                     /locus_tag="VFM00_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HET7496899.1"
                     /translation="MSHTISHPAERGVPRAGEHRGLLDRTLGLVADVRGGEGVTALLL
                     MLNIFFLLASYYLLKTIREPLILSQPGGAEVKSYAAAAIAGVLIVLVPIYSAIASRVS
                     RVKLINGVTLFFILCLIGFFMADRIGYAVGTIFFIWVGIFNLMVIAQLWAFANDVYTV
                     DQGKRLFAIVGVGASLGAIAGSFVTGKLVKAFGPYPFMLAAAAILGICMVLTNLINLR
                     EKRGVEGQPETPGDKAAAADAPAAGDEKDAKRSGFALVLTNKYLLLIAALMILSNLVN
                     TTGEYILGKTIVNVLSARQGGALDEEKAIGAFYGNYFTIVNIISFAIQAFVVSRILKW
                     FGVRTALLVLPFVALIGYASMAFVPVLSFIRGAKIAENSLDYSLQNTTRNALYLPTSR
                     AAKYKAKQANDTFFVRLGDVLSAGLVFAGTTWLAFKPTDFARVALALILIWLGIAFAL
                     GRMFQRQAKTA"
     gene            complement(4220..5842)
                     /locus_tag="VFM00_01245"
     CDS             complement(4220..5842)
                     /locus_tag="VFM00_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7496900.1"
                     /translation="MSDATPPRAGGAWIRPSAFFLAAALVLAAGCSSTGRLSGVVQTV
                     SPPAADSIPRTAVRGEGEKRVAREDVLGTWSLFTSDTLIGFIPGTGDSTRRYLGRLRR
                     GYTADTLNVLLCGDNRPAYRTTRLKEDLLKVEHIFSLNPLNIARGLVAIPTVLVKGMV
                     PDLALVRDVPDVLRKHPTWGREVSVNKAIGAKLDSLEGTGQAVAAIINTGDLVKDGRI
                     PAQWKRFLRLIRPLSARVPYFGIAGNHERTDTPLGVANWRTATGLPATGDRLYYCFDS
                     ADGWVRFLALDSNPMTDPANHWPRDVEIKYSDEQIDWLVARIKEHRGPAFVFMHHPPL
                     SVGFHRVEWQSDAVLQERRERMVRALHENGIGVLATGHEHAYERALMTWPNGVLINIV
                     TGGAGSPLHDIPPREESARLFSEYKVAGGVIKPENVLTGQFFHFIHVRLWFGGGEFTT
                     YEVLPDGKVRLADHVQIDLKRYGVPKIDQHKVPIPAEPKSQPPPTEENPKAKGLISTT
                     KSDSVSAVSKTIKNTPPPSRAKTPVRPRRIRR"
     gene            complement(5839..7551)
                     /locus_tag="VFM00_01250"
     CDS             complement(5839..7551)
                     /locus_tag="VFM00_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496901.1"
                     /translation="MRPTTTNIPTRRAILRLLAALIGVALSAWVSARAMAADGRHEAE
                     ERLERQPVKASTWVDHDGQPVPQPHVSDPSHAGHLVRESFVEPVSHLFDIPDKIIWLM
                     RPLGVRRVPDAPNVNAYDEVGNSSWFTNRNHVRALSTDAIREGPFGPVRPTPPYTIKS
                     VKTHGFNPGFNMKDAAGKRWVVKLDRVGFPQISSGAGVVSSRLVWAAGYNISHDEAFT
                     FHRDELSIDSDLVNGKDGKKPFRDADLEALLKRGARTEDGRYYASASFFLPGTPIGPF
                     SFRGIRQDDPNDRFRHKERRDLRGLFVVYSWINNWDVKDDQSLDTFGPDSANGHVTHY
                     LLDVNGSLGAAAEGPKPLKYGYEQRFDTGRILQRFVTLGFSTDPWRRARQDSGIPSVG
                     NFEAVEFDPADWAPLQYMEPFRRMTLADAYWGAKIVASFSNAQIAAAVDAAGYEDPRA
                     REYIQRTLIARRDKIARFWFARVAPLDFFHVKNGYLVFHDLAVDLGLTPERGYEVEER
                     GGGGSVPVEVLARDPRISLGRLGRGTDEISLKLEVAGSHARPVRVDLRRSGHDWVVTR
                     VRHG"
     gene            complement(7551..8891)
                     /locus_tag="VFM00_01255"
     CDS             complement(7551..8891)
                     /locus_tag="VFM00_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496902.1"
                     /translation="MPTRTNHRSLVMPAFVVILAAIFTFGASPSRAEDRFGDSTWVAP
                     IPEASGAPEDPGPRVAEPDHERTWETVLRTPFRVAFFPFRALGWGLEGAADLAEKHLP
                     VDELNAPPGPPHGFHAAPQPSLSSNEGLGFGVSMKAALGVPQNLFRSDLKWTTKDTRR
                     ARATFLLGADRPVGYSLFGLYRHRPNRRFYGIGNDAGTTRTIFLEREDEFVGSVLVAH
                     KPKLNARGYVGLSDIDIGPGYNDSPRSIEVFSPAEVPYLTRGSRVWFVGAGAGWASLN
                     EPYDPARGVQIFGEARHVMDADGNDLTYEAYRVEGRGYLPVFADRRILVGRAVLDGVN
                     PASGSGPIPFYRIPFSNGDDRFSGYSGDRFRDKRLVILQAEYRWLIWSKLWAFALAQR
                     ASVAPSTGALRWASMHEAYGGGFRYRITDTQTARLELAKGNQGFDIDLNLEAPF"
     gene            9200..11917
                     /locus_tag="VFM00_01260"
     CDS             9200..11917
                     /locus_tag="VFM00_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HET7496903.1"
                     /translation="MALTRRLGLEPEEQRTFYLMGLLVAALLCAYTLAKVVRDALFLA
                     NFGALALPYAYVGVALASAAFIWMEGRLTRRYPRLDLTRFNQRLAIAFGVAAAITYPL
                     ARTWTTAAFYLWTGSQAMLLIPHFWVLALDVWDSHRARQLFPILSGCGLIGGLLGGGI
                     AGWMTPIFKRVGLIWIVAGLFVVVHGLTLVLQRHRAKRSSPIEFASTLSRWEILRRSG
                     YLRLLAVALALAVIVGTLIDFQFKFYIQRIYPDPHALTQFLGRFQVALNALALLVQFG
                     AVGYVFHRLGLANASLLQPTSVMIFAGWVAATGGWWAIVAMRWVQGVVLQTLGKSTSE
                     IYYMAVRPPERRRIKPAVDTLVERWSDAVVGVLLIALLKAVGIAIPVLIGTTLAIAVV
                     WLVVLFFLNRQYGRAFQEALSRRWVEPELAPESMQTRAAEQALLTALRSGNEPQMITA
                     LRLAEASRNRAVAAGVRGAIEHRSPAVRAAAVRAAEALRMRAAAPLIEPLERDPDEAV
                     AGAALRFGLAMSRNSVAYARARLEGDDSLLCQRTVEALFERPHAAPGALTIEWIDRRI
                     EKGDTESLVLAARAVGATPGSAARSRLPALLADPDVEVKRAALLAAARRPTAVLLDPI
                     LPLLFVPDVGGEARAALAAIGDPAVPSLMRFLTSEHSARARAIAVRTLAELGSPRAVK
                     ALLGLTRSSDPNERTLGLRSLSRVRVSTGRPVLPRALVHRLFLRELRSYRSSIEPAVE
                     LEHASDPEIRLLAESWVEYGEGALERAMRALACWYDPQPVSGAFERLKSRDYEKVAPA
                     LEYLVHVLPRPVFRPLREIFDARMVRERTRGEDGPTHLPDWIRAAWETGDAWMRACAV
                     RASWVVIEADRTWFTGGESSAMVDAELAARFPDPGVGPVARVAGGAASC"
     gene            11911..12606
                     /locus_tag="VFM00_01265"
     CDS             11911..12606
                     /locus_tag="VFM00_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7496904.1"
                     /translation="MLTPVERVLILKGADLLRGVGPRHLLGLANVSREVPIYAGDTIY
                     LETDPADALYIVVEGRVRISTAGRATSEVGPGEAFGTWSLIDDSERGQQAECIEDGLA
                     LSLSREEFYDVASADLAILQELVRVLAKRLRELAATAPPEEARVEGEGIEKTEAQVEA
                     ARPTESPAEAVGPTSSPGSSLAAAAAGKATTSETPLTAPNLPGPTDGTAALDPVIAPE
                     PIVPPTESVETEE"
     gene            complement(13181..14533)
                     /locus_tag="VFM00_01270"
     CDS             complement(13181..14533)
                     /locus_tag="VFM00_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7496905.1"
                     /translation="MPVALVVDDEPNSLSALAELVESEGFATVTAGTLADARSRLSQT
                     PPDVVLVDLMLPDGSGLDLFAELDPTHRTEVILITGNATVDSAVAALRTGVLDYLTKP
                     VDFRRLKTVLQNVSRTLALKEEIGTLRDELRQLGRFGRLIGNSSAMQKVYDLIVRVSP
                     TDATVLLVGLSGTGKEEVAATIHEVGRRKKQPFLPLNCGAVPPNLMESELFGHERGSF
                     TGAAQMHRGYFERASGGTLFLDEITEMPLDLQVKLLRVLETGTIMRVGGDGLIEVDVR
                     VIAASNRSPEAAVKEGKFREDLLYRLNVFPITLPPLKERNGDIELLAEHFLAQINREE
                     GSTKRFSPNARRRMAAYAWPGNVRELKNLIRREYIMSDEVIEMDALPTNPVAAVAAAD
                     GGTPDAIKVGMSLSDIERYFILATLEQYGGDKRKAAEVLGISLKTMYNRLNNYAAAVQ
                     "
     gene            complement(14550..15242)
                     /locus_tag="VFM00_01275"
     CDS             complement(14550..15242)
                     /locus_tag="VFM00_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496906.1"
                     /translation="MPDPLTPGDDATLARHLRNLGRMYPVLAHDLHAFLNTRVLNLEL
                     LQRAAAKEDPDPQTMARIRRFAALVADEIPPLDRMLKAVVGQMRLSETPGTRFDFRTL
                     CEELAIVFDSYARYRRMRLKATLADVPMAVAGDRDAMAHALTALLLDMLEAAPQGTDL
                     TLSLQADRRLATLTLAAAPPAVLTREPAVPEGAAAAREVLERYGARIDDSPGADGSSA
                     LGITVPLVPLIS"
BASE COUNT         2351 a         5338 c         5584 g         2430 t
ORIGIN      
        1 cggggcgcac ggtcccggcg ccgtcttggt ggacgtccgc gcgctgggcg cgatcggcgc
       61 cgactggtac gccgcgaacc tccacaagtg ggcgttcgcg ccccggagct gcggcattct
      121 ctgggccgcg ccggagcggc aggaggagct gcatccgccg gtgatctcct ggggcatcgg
      181 ccgcgggttc ctggcggagt tcgactgggt gggcacgcgc gatccctcgg cctggctcgc
      241 cacgccggcc gcgctcgact ggttcgagga gctgggcgcg gaggcggtgc gcgagcacaa
      301 ccgcgccctg gcgcgccgcg cccgctcgct cctgaccgag cgctggggga cggagcccgc
      361 ggctcccgag gcgatgaccg gccccatgac gacgattccg atgccggatc ggctcggaag
      421 cgagccggag gacgccgcgc gcgtgcgcga ccggcttctc ttcgaggacc ggatcgaggt
      481 gcaggtgcac gcgtggcgag ggcggatctg ggcgcgcgtc tcggcccagc tctacaacga
      541 cgacgacgac ctggagcggc tagcggcggc ggtcgcggcg ctccattcct gaaggagcct
      601 cgatgccgac gaaacaatca gcgaagaaca ccctgcgcgc cgcgaagggc gctgagccct
      661 ccgcaaagcg gggctccggt tatgggatgc ccgccaggaa ctcgtggttc acgcgcttcg
      721 ccaaggcgac ctcgcgtagc acgggccgcc cgatcgcgtt cgccctggcg gcggcgatca
      781 tcgtgctctg ggccatgacc ggtccggtct tccatttcag cgacacgtgg cagctcgtca
      841 tcaacacggg caccacgatc atcaccttcc tcatggtctt cctcatccag aacacgcagt
      901 accgcgactc ggaagcggtc cacctgaagc tggacgagct gatccgggcc caggaggggg
      961 cgcacaacgc gctgctcgac ctggaggacc tggacgacaa ggagctggac cggatccggg
     1021 agaactacgc ccagctggcc gacgacgcgc ggaagatatt gcgccgccgg ggcgtggaca
     1081 cgggcacccc ggacgtgaag aacggccgct gagccggtgt tgaaccgctg cgctccagaa
     1141 gcgtagactt gacggcaggg gaagtgggct cctggggtct cgcggaagag gagaccgagc
     1201 catgaaaagc gactccggcg ccacccgttc ggtgtggatg gacgtcccgc cgctgcctgc
     1261 ccgacccccg ctcgaccggg acctgcgcgc tgatgtctgc gtcgtgggcg ccggcatggc
     1321 gggcctcacg atcgcctacc tgctcgctcg cgaagggctc cgcgtcgccg tcctcgacga
     1381 cggcccgacc gcgggcggag aaacctcccg gaccaccgca cacctcacga acgtgatcga
     1441 cgatcgctac cattggatcg agcaggtgca cggccccgaa ggggcgcgcc tcgcggccga
     1501 gagccacacc gcggcgatcg acacgatcga ggcgatcgcc gagcgggaac agatcgcgtg
     1561 cgacctggtc cgtctcgacg cctatctctt cgcgggaccc ggcgatcccc gtgacgagct
     1621 ggagcgcgag ctggaggcgg cccgtcgcgc gggcctcacg ggcgttgcgt gggtcgaccg
     1681 ggcgcccatc gaggggttcg ccacgggacc ctgtctgcgg tttccaaacc aggcgcagtt
     1741 ccatccgctc cgctacctgc agggcgtggc cgatgcgatc gagcgctccg gaggacgcat
     1801 ccacggcggc acccacgtga ccgcggtgca ccacggtccg ccgccgcgcg tcgagacgga
     1861 gagcggcgtc gcggtcgtcg cggacgccat cgtggtcgcc accaactccc ccgtgcacga
     1921 gatgatcggc gtccacccga agcaggccgc ctaccgcacc tacgtggtgg ccgcccgcgt
     1981 gccgcgcggc gtggtgacgc gcgcgctcta ctacgacacg gagaatccct accactacgt
     2041 ccggctgcag cccgagagcg cgggaggccc ggatctcctg atcgtgggcg gcgaggacca
     2101 caagacggga caggccgacg atgccgacga gcgctgggcc gccctggagg cgtggacgcg
     2161 cgagcgcttc ccgctggcgc gcgagttcgt cgcgcggtgg tcggggcagg tgatggagac
     2221 gatggacggc gtggccttca tcggcccgga ccgggccggc tcgcgggtct tcatcgcgac
     2281 cggggactcg gggatgggga tgacgcactc caccatcgcc gggctcctcc tccgcgacct
     2341 catcgcgggc cgcgagaatc cctgggcgca tctctacgac ccgggccggg tcagcctgcg
     2401 cgccgcgggc gagctggcca aggagaacct gaacttcgtg tccaagtacg cggactacgt
     2461 gaccggcggc gacgtcggct ccgccgatga gatcccgccg ggcgagggcg ccgtgctgcg
     2521 ccgcggcctg accaaggtgg cggcctatcg cgacccctcg gggacgctgc acgagatgtc
     2581 ggcggtctgc acccacctgg ggtgcgtggt gcggtggaac tcggccgaga agagctggga
     2641 ctgcccctgc cacggctccc ggttcgacag ccacggccgc gtcgtgaacg gacccgcgac
     2701 gcgcgatctc gccgagaagg acaccgccca gcccgccgcg gcctgggaac gggcgtgacc
     2761 aaggctcggg cccgcggcgg acgcctcccg ccgcgggccg gcgggccgcc ctaggccgtc
     2821 ttcgcctgcc gctggaacat gcggcccagc gcgaacgcga tcccgagcca gatgaggatc
     2881 agcgcgagcg ccacccgggc gaagtcggtc ggcttgaacg ccagccacgt cgtgcccgcg
     2941 aagaccagcc ccgccgagag cacgtccccc agccgcacga agaaggtgtc gttcgcctgc
     3001 ttcgccttgt acttcgcggc acggctcgtg ggcaggtaga gcgcgttccg cgtcgtgttc
     3061 tgcagcgagt agtcgagcga gttctcggcg atcttggcgc cgcggatgaa cgagaggacc
     3121 gggacgaacg ccatcgaggc gtagccgatc agcgccacga aggggagcac gagcagcgcg
     3181 gtgcgcaccc cgaaccactt gagaatgcgc gacacgacga acgcctggat cgcgaaggag
     3241 atgatgttca cgatggtgaa gtagttgccg tagaaggcgc cgatcgcctt ctcctcgtcc
     3301 agggccccgc cctggcgcgc cgacagcacg ttcacgatcg tcttgcccag gatgtactcg
     3361 cccgtggtgt tcaccaggtt cgagaggatc atcagcgccg cgatcaggag caggtacttg
     3421 ttggtgagga cgagcgcgaa cccgctccgc ttggcgtcct tctcgtcgcc ggccgccggc
     3481 gcgtccgccg ccgccgcctt gtcgccgggc gtctccggct gcccctccac gccccgcttc
     3541 tcgcgaaggt tgatcaggtt cgtcagcacc atgcagatcc cgaggatcgc cgcggccgcc
     3601 agcatgaagg ggtaggggcc gaaggccttg accagctttc cggtcacgaa cgagcccgcg
     3661 atggcgccga gcgaggcgcc gacgcccacg atcgcgaaga gccgctttcc ctggtccacg
     3721 gtgtagacgt cgttggcgaa cgcccagagc tgcgcgatca ccatcaggtt gaagatgccg
     3781 acccagatga agaagatcgt gcccaccgcg tagccgatgc ggtcggccat gaagaagccg
     3841 atcaggcaca ggatgaagaa gagcgtgacg ccgttgatca gcttgacgcg ggagacgcgc
     3901 gaggcgatcg cgctgtagat cggcaccagc acgatcagca ccccggcgat cgcggccgcg
     3961 gcgtagctct tcacctcggc gcctccgggc tgcgacagga tgagcggctc gcggatggtc
     4021 ttgagcaggt agtaggaggc gagcaggaag aagatgttca gcatgaggag gagcgccgtg
     4081 acgccctcgc ccccgcgcac gtcggcgacg aggccgaggg tgcggtcgag cagcccccgg
     4141 tgttcgccgg cgcggggcac gcctcgctcg gcgggatggg aaatcgtgtg gctcatggat
     4201 tcctccgtgg tatcgccgct cagcggcgga tccgccgggg ccgcacgggc gttttggcgc
     4261 ggctgggcgg cggcgtgttc ttgatcgtct tcgagacggc ggacaccgag tcggatttcg
     4321 tggtcgagat gaggcccttg gccttgggat tttcctcggt cggcggcggc tgggacttgg
     4381 gctccgccgg gatcgggacc ttgtgctgat cgatcttcgg gaccccgtag cgcttcaggt
     4441 cgatctggac gtggtccgcc aggcggacct tcccgtcggg cagcacttcg taggtggtga
     4501 actcgccgcc tccgaaccag agacggacgt ggatgaaatg gaagaactgc ccggtcagga
     4561 cgttctcggg cttgatgacg ccgccggcca ccttgtactc gctgaagagg cgcgcgctct
     4621 cctcgcgcgg cgggatgtcg tgcagcgggg agccggcgcc tccggtgacg atgttgatca
     4681 ggacgccgtt cggccaggtc atgagcgcgc gttcgtaggc gtgctcgtgc cccgtcgcca
     4741 gcaccccgat cccgttctcg tgcagcgccc gcaccatccg ctcacgcctt tcttgaagaa
     4801 ccgcgtcgct ctgccactcg acgcgatgga acccgaccga gaggggagga tggtgcatga
     4861 agacgaaggc gggaccgcgg tgctccttga tgcgcgccac cagccagtcg atctgctcgt
     4921 cggaatactt gatctcgacg tcgcgcggcc agtggttcgc ggggtcggtc atggggttgg
     4981 agtcgagcgc caggaaccgc acccagccgt cggcggaatc gaagcagtag tagagacggt
     5041 cgccggtcgc ggggaggccc gtcgccgtgc gccaattcgc cacccccagc ggcgtgtcgg
     5101 tccgctcgtg gtttccggcg atcccgaagt aggggaccct cgccgagagc ggccggatca
     5161 ggcggaggaa gcgcttccac tgcgccggga tgcggccgtc cttgaccagg tcgccggtgt
     5221 tgatgatcgc ggcgaccgcc tggcccgttc cctccagcga gtccagcttg gccccgatcg
     5281 ccttgttgac cgagacctcg cgtccccacg tcgggtgttt ccggagcacg tcgggcacgt
     5341 cgcgaaccag ggcgaggtcg ggaaccatcc ctttcacgag caccgtcggg atcgcgacca
     5401 ggccgcgcgc gatgttcaac gggttgaggg agaagatgtg ctcgaccttg aggaggtcct
     5461 ccttgaggcg cgtggtgcgg tacgccggac ggttgtcgcc gcagaggagg acgttgaggg
     5521 tgtcggccgt gtagccgcgg cgcagccggc ccaggtagcg ccgcgtgctg tcgcccgtcc
     5581 ccggaatgaa gccgatcagc gtgtcgctgg tgaagagcga ccaggtcccg agaacgtcct
     5641 cgcgggccac gcgcttctcc ccttcgcccc ggacggccgt gcggggaatg ctgtccgcgg
     5701 ccggcggcga gaccgtctgg accacgccgc tcaggcgccc cgtggaggag caccctgccg
     5761 ccagcaccag cgccgcggcc aggaagaagg cggagggtcg aatccaggcg cctccggctc
     5821 ggggaggcgt cgcgtcgctc atccgtggcg gacccgcgtc acgacccagt cgtgcccgct
     5881 ccgccgcagg tcgacccgta ccggccgcgc atggctcccg gccacttcga gcttcaggga
     5941 gatctcatcc gttccgcggc ccagccggcc cagactgatc cgcgggtcgc gcgccaggac
     6001 ctcgaccggc accgacccgc cgccgccgcg ctcctcgacc tcgtagccgc gctcgggggt
     6061 caaccccagg tccaccgcca ggtcgtggaa cacgaggtag ccgttcttca cgtggaagaa
     6121 gtcgagcggg gccacccgcg cgaaccagaa gcgggcgatc ttgtcgcgcc gggcgatgag
     6181 cgtccgctgg atgtactccc gcgcgcgggg gtcctcgtaa cccgcggcat ccaccgcggc
     6241 tgcgatctgg gcgttggaga aggaggccac gatcttggcg ccccagtagg cgtcggccag
     6301 cgtcatgcgg cggaacggct ccatgtactg gagcggcgcc cagtcggcgg gatcgaattc
     6361 caccgcctcg aaattgccga ccgaggggat cccgctatcc tggcgcgcgc ggcgccaggg
     6421 atcggtcgag aatcccagcg tgacgaaccg ctggaggatc cggccggtgt cgaagcgctg
     6481 ctcgtagccg tacttgagcg gcttgggtcc ctcggcggcc gcgcccagcg aaccgttcac
     6541 gtccaggaga tagtgggtga cgtggccgtt cgcgctgtcg ggtccgaacg tgtccaggga
     6601 ctggtcgtcc ttcacgtccc agttgttgat ccacgaatag accacgaaga gaccgcgcag
     6661 gtcgcgccgc tccttgtggc ggaaccggtc gttcgggtcg tcctggcgga tgccccggaa
     6721 gctgaacggg ccgatcggcg tgcccggaag gaagaagctc gcgctggcgt agtagcgccc
     6781 gtcctcggtg cgcgcgccgc gcttcaagag cgcctcgagg tcggcgtcgc ggaacggctt
     6841 ctttccgtcc ttgccgttta ccaggtcgga gtcgatcgac agctcgtccc ggtggaacgt
     6901 gaacgcctcg tcgtgcgaga tgttgtaccc ggcggcccag acgagccggc tcgagacgac
     6961 gccggcgccc gaggagatct gcgggaagcc gacacgatcc agcttcacga cccagcgctt
     7021 gccggccgcg tccttcatgt tgaagccggg attgaagccg tgcgtcttca cgctcttgat
     7081 cgtgtacgga ggcgtgggac gcacgggtcc gaagggccct tcccggatcg cgtccgtcga
     7141 gagcgcccgg acatggttgc ggttggtgaa ccagctggaa ttgccgactt cgtcgtaggc
     7201 gttcacgttg ggcgcatcgg gcacccggcg cacgccgagc ggcctcatca gccagatgat
     7261 cttgtcgggg atgtcgaaca gatgggagac cggctccacg aaggactcac ggaccaggtg
     7321 gcccgcatgg ctcgggtccg acacgtgcgg ctgcggaacc ggctggccgt cgtggtccac
     7381 ccaggtgctc gccttgaccg gctgacgctc caggcgctct tccgcttcat gccggccatc
     7441 cgcggccatg gcgcgcgcgc tgacccaggc ggacagggcc acgccgatca gggccgcgag
     7501 caggcgaagg atcgcgcgcc gcgtcgggat gttcgtcgtc gtcgggcgca tcagaagggt
     7561 gcctccagat tcaggtcgat gtcgaatccc tgattcccct tcgccagctc gaggcgggcg
     7621 gtctgggtgt cggtgatgcg gtagcggaag ccgccgccgt aggcctcgtg catcgaggcc
     7681 caacgaagcg ccccggtgct cggcgcgacc gaggcgcgct gcgccagggc gaacgcccag
     7741 agcttggacc agatcagcca gcgatattcg gcctgcagga tgacgagacg cttgtcccgg
     7801 aaccgatcgc ccgagtaccc cgagaagcgg tcgtccccgt tcgaaaaggg gatccggtag
     7861 aaggggatcg ggccggagcc gcttgccgga ttcacgccgt cgaggacggc gcggccgacc
     7921 aggatccgcc ggtccgcgaa gaccggcagg tagccccgcc cctcgacgcg gtacgcctcg
     7981 taggtcaggt cgttgccgtc ggcgtccatg acgtggcgcg cctcgccgaa gatctggact
     8041 ccgcgcgcgg gatcgtacgg ctcgttcagc gaggcccatc cggcgccggc gccgacaaac
     8101 cagacgcgcg atccgcgggt gaggtaggga acttcggccg gcgagaacac ttcgatgctg
     8161 cgcggcgaat cgttgtaccc gggtccgatg tcgatgtccg agaggccgac gtaccctcgc
     8221 gcgttgagct ttggcttgtg ggccacgagc acggagccca cgaattcgtc ttcccgctcg
     8281 agaaagatgg tccgggtcgt tccggcatcg ttcccgatcc catagaagcg gcggttcgga
     8341 cgatggcggt acaggccgaa cagcgagtaa ccgacggggc ggtccgcgcc gagcaggaac
     8401 gtggcgcgcg cacggcgcgt gtccttcgtg gtccacttca gatccgagcg gaacaggttt
     8461 tggggtaccc cgagagctgc cttcatggag acgccgaaac cgagaccctc gttgctcgag
     8521 agggagggct gcggtgcggc gtggaatccg tgcggcggcc ccggcggcgc gttgagctca
     8581 tcgacgggga ggtgtttctc ggccaggtcc gcggcgccct ccagccccca gcccaaggcg
     8641 cggaagggga agaaagccac gcgaaacggc gtgcgcagca cggtctccca ggtccgctca
     8701 tggtcgggct cggcgacgcg cggtcccggg tcttcgggag cgccggaagc ctctgggatc
     8761 ggagcgaccc acgtggaatc gccgaaccga tcctccgcgc gggaagggga tgccccgaac
     8821 gtgaagatag ccgcgaggat gacgacgaac gcgggcatga cgagggagcg atgattcgtt
     8881 ctggtcggca cagggcctcc gggtgcggag agaactgatg cggctcgtgc gagccgtgcc
     8941 cttatcaagg cacgtgccga agcggcttcc agccgggacg ctagcagtta cccacccgaa
     9001 aggaaaagaa ctcgttgtca gtttttccct gatggcacgg ccagggatcg tatttacaag
     9061 gcgagggcgg gtggcgcgag ggcgttggcc cgacctgtgc aggaggctgc gccatctcac
     9121 ctcctcggcg cgtttgcgcc cagggcactg gtgacccccg cgccgcgaac aggaggattc
     9181 catcgcatcc cgtctcacga tggccctgac ccgccgtctc ggactcgagc cggaggagca
     9241 gaggacgttc tacctgatgg gcctcctggt ggcggcgctt ctctgcgcct acaccctggc
     9301 caaggtggtg cgcgacgcgc tcttcctcgc gaacttcggc gccctggcgc ttccgtacgc
     9361 ctacgtaggc gtggcgctcg cttcggccgc cttcatctgg atggaggggc gcctgacgcg
     9421 gcgctacccg cggctcgacc tgacgcggtt caaccagcgc ctggcgatcg ccttcggagt
     9481 cgcggccgcc atcacctatc cgctcgcccg gacctggacc acggcggcct tctatctctg
     9541 gaccggaagc caggcgatgc tcctgatccc ccacttctgg gtgctcgctc tggacgtgtg
     9601 ggactcgcat cgcgcccggc agctctttcc gatcctgagc ggctgcggcc tcatcggcgg
     9661 cctcctggga ggcggcatcg cgggctggat gacgccgatc ttcaagcgcg tcgggctcat
     9721 ctggatcgtg gccggtctct tcgtggtcgt gcatgggctg acgctggtgc tccagcgaca
     9781 ccgcgccaag cggagcagcc cgatcgagtt cgcttcgacg ctctcccgct gggagatcct
     9841 gcgccgctca ggctatctcc ggctcctcgc ggtcgccctc gcgctggcgg tgatcgtggg
     9901 cacgctgatc gactttcagt tcaagttcta catccagcgg atctatcccg acccgcacgc
     9961 gctgacccag ttcctcggcc gcttccaggt ggcgctcaac gcgctcgcgc tgctcgttca
    10021 gttcggcgcg gtggggtacg tgttccatcg cctcggcctc gccaacgcca gcctgctcca
    10081 gccgacgtcg gtcatgatct tcgccgggtg ggtcgccgcg accggagggt ggtgggccat
    10141 cgtggcgatg cggtgggtgc agggagtcgt gctgcagacg ctcgggaaat ccacgtcgga
    10201 gatctactac atggcggttc gcccgcccga gcgaaggcgc atcaagccgg ccgtggatac
    10261 cctggtcgag cggtggtccg acgcggtcgt cggcgtgctc ctgatcgcgc tcttgaaggc
    10321 ggtcgggatc gcgattccgg tcctgatcgg aacgacgctc gccatcgccg tcgtctggct
    10381 ggtggtcctg ttcttcctga accggcaata cgggcgcgcg ttccaggaag cgctgagccg
    10441 ccgctgggtc gagccggagc tggctcccga atcgatgcag acgcgcgccg ccgaacaggc
    10501 gctgctcacg gcgctgcgtt cggggaacga gccgcagatg atcaccgcgc tccgtctcgc
    10561 ggaagcgtcg cggaaccgtg ccgtcgccgc cggcgttcgc ggcgccatcg agcaccggtc
    10621 gcccgcggtc cgggccgcgg ccgtccgcgc ggcggaggcg ctccggatgc gcgcggccgc
    10681 cccgctcatc gaaccgctcg agcgcgaccc ggacgaggcg gtggcgggcg cggcgctccg
    10741 cttcggactg gcgatgagcc ggaattccgt cgcctacgcg cgggcgcggc tggagggcga
    10801 tgactcgctc ctctgccagc gcacggtcga agcgctgttc gagcggccgc acgccgcgcc
    10861 cggcgcgctg acgatcgaat ggatcgaccg ccggatcgag aagggagaca ccgaaagcct
    10921 tgtcctggcc gcgcgcgcgg tcggagccac gccgggaagc gcggcgcggt cccggctgcc
    10981 ggcgctcctg gccgaccccg acgtcgaggt gaagcgggcg gcgctcctcg cggccgcgcg
    11041 ccggccgacg gcggtgctcc tcgacccgat cctcccgctc ctcttcgtgc cggacgtggg
    11101 cggcgaggcg cgcgccgcgc tggcggcgat cggggacccg gcggtgccga gcttgatgcg
    11161 attcctgacc agcgagcaca gcgcgcgcgc ccgcgccatc gcggtgcgca cgctggccga
    11221 gctggggagc ccgcgcgccg tgaaggcgct gctcggcctg acgcgcagca gcgatcccaa
    11281 cgaacgaacg ctgggtcttc gcagcctgag ccgcgtgcgc gtgagcacgg gccgtcccgt
    11341 gcttccgcgc gcgctcgtcc atcgcctttt cctgcgcgag ctgcggagct accgctcgtc
    11401 gatcgagccg gccgtcgagc tggagcacgc gtcggacccg gaaatccgtc tcctcgccga
    11461 gagctgggtc gaatacgggg aaggggcgct cgagcgcgcg atgcgcgcgc tggcctgctg
    11521 gtacgacccc cagccggtgt cgggggcttt cgagcggctc aagtcgcggg actacgagaa
    11581 ggtggcgccc gcgctcgagt acctcgtgca cgtgctgccg cgccccgtgt tccggccgct
    11641 tcgcgagatc ttcgacgccc gcatggtgcg ggagcgcacg cggggcgaag acggtccgac
    11701 ccacctcccg gactggatcc gggcggcgtg ggagacgggc gacgcgtgga tgcgcgcgtg
    11761 cgccgtgcgc gcgtcgtggg tggtcatcga ggccgaccga acgtggttta caggcggcga
    11821 atccagcgcc atggtggacg ctgagctggc ggcgcgcttc cccgatcccg gggtgggacc
    11881 ggtggcgcgc gtcgcgggag gagcggcctc gtgctgacgc cggtggaacg cgtgctgatc
    11941 ttgaagggtg cggatctcct gcgcggcgtg gggccgcggc atctcctggg cctggccaac
    12001 gtctcgcgcg aggtgccgat ctacgcgggg gacacgatct atctcgagac cgacccggcg
    12061 gacgccctgt acatcgtcgt cgagggacgc gtgcggatct ccacggccgg gcgcgccacg
    12121 tcggaggtgg gacccgggga ggcgttcggg acctggtcgt tgatcgacga ctccgagcgc
    12181 ggccagcagg ccgagtgcat cgaggacggt ctcgcgctct cgctctcgcg cgaggagttc
    12241 tacgacgtcg cgtccgccga cctggccatc ctccaggaat tggtgcgcgt gctggccaag
    12301 cgccttcgcg agctggcggc cacggcgccg cccgaagagg cccgcgtcga gggagaaggg
    12361 atcgagaaga ccgaggcgca ggtggaagcg gcgcgcccca ccgagagccc cgccgaagcg
    12421 gtcgggccca cctcgtcccc cggctcgtcg ctcgccgcgg cggccgcggg aaaggcgacg
    12481 acctcggaaa cgcccctcac cgcgcccaac ttgcccggcc cgacggatgg aacggcggct
    12541 ctcgaccccg tcatcgcacc ggaaccgatc gtgccaccta cggaaagcgt tgaaacggaa
    12601 gaatagtgtg cggaggttgc ctcgaagggc taccgagcgc actgcgtttt ggcgcgaggg
    12661 gaggcaacgc tccgggcgct tgtcctcgcc cggccccctc gctctcgctc cgcgagagct
    12721 ccgggggacg cgggctgcgg aggcgccctg cgcgttcctc ccctcgcgtc caagaccgcg
    12781 gttcgacctg cggacgggat cgaaacgagc ggggcgaggc tcgctttgct cgctcgctcc
    12841 aactacctca agttcggagc cggtccctgt gtcctcatgg ctccctcatg tcacgggata
    12901 cggagccgca gcatggaaga gatcacgctc atcgtctgaa atcatgatga gagtagcggg
    12961 ccgcgtcgtg tttggggacg cgaggggagg aacgcgcagg gcgcctccgc agcccggata
    13021 ccccggagcc gagcgcagcg aggcgagggg tccgggcgag gacaagcgcc cggagcgttg
    13081 cctcccctcg cgccaaaacg cagtgcgccc ggctctcttt cgagaagccg ggcgcatggg
    13141 cgtcttttgt ctttttcttt gtcttggtct tgcgacgacg ctactgcacc gccgccgcgt
    13201 agttattgag cctgttgtac atcgtcttga ggctgatccc caacacctcc gccgccttcc
    13261 gcttgtcccc gccgtactgc tccagcgtgg ccaggatgaa gtagcgctcg atgtcggaga
    13321 gcgacatccc caccttgatc gcgtcggggg tgccgccgtc ggctgccgcg acggccgcga
    13381 ccgggttggt cggcagcgcg tccatctcga tcacctcgtc cgacatgatg tactcgcggc
    13441 ggatcaggtt cttcagctcg cgcacgttcc cgggccatgc gtaggccgcc atgcgccggc
    13501 gcgcgttcgg gctgaagcgc ttcgtggatc cctcctcgcg gttgatctgc gcgaggaagt
    13561 gctcggccag gagctcgatg tcgccgttcc gctccttgag cggcgggagc gtgatcggga
    13621 acacgttcag ccggtagagg agatcctccc ggaacttccc ctccttcacg gccgcttccg
    13681 gagaacggtt gctcgcggcg atgacgcgga cgtccacctc gatcagccca tcgccgccga
    13741 cccgcatgat cgtcccggtc tccagcacgc ggagcagctt gacctggagg tcgaggggca
    13801 tctcggtgat ctcgtccagg aagagcgtgc cgccggaggc ccgctcgaaa tagccgcgat
    13861 gcatctgagc ggcgcccgtg aagctgccgc gctcgtgtcc gaacaactcg ctctccatga
    13921 gattgggcgg caccgcgccg cagttgagcg gcaggaaggg ctgcttcttg cgccggccga
    13981 cttcgtggat ggtcgcggcc acctcttcct ttccggtccc gctcagcccg acgaggagca
    14041 ccgtggcgtc ggtgggcgac acgcgcacga tcaggtcgta gaccttctgc atcgcggagg
    14101 agttgccgat cagccggccg aagcggccca gctggcgcag ctcgtcgcgc agcgttccga
    14161 tctcctcctt gagcgcgagg gtgcgcgaga cgttctggag aaccgttttc aggcgccgga
    14221 agtcgaccgg cttggtcaga tagtccagca cgccggtccg gagcgcggcg accgcggagt
    14281 ccaccgtcgc attgcccgtg atcaggatca cttcggtgcg gtgcgtggga tcgagctcgg
    14341 cgaacaggtc gagtccgctt ccgtccggca gcatcaggtc caccaggacg acgtccggag
    14401 gcgtctggct cagacggctc cgcgcgtcgg ccagcgttcc cgccgtgacc gtggcgaaac
    14461 cttcgctctc gaccagctcc gcaagcgcgg agaggctgtt cggctcgtca tcgacgacca
    14521 gggcgacagg catagacgac ggatctcctt tatgaaatca aggggacgag gggcacggtg
    14581 atgccgagcg cggaggagcc gtccgccccg ggggaatcgt cgatccgggc gccgtagcgc
    14641 tccaggacct cgcgcgcggc cgccgcgccc tcgggcacgg cgggctcccg cgtcaggacc
    14701 gccggcggcg cggccgccag cgtcagcgtc gccaggcggc ggtccgcctg cagcgacaac
    14761 gtcagatcgg tcccctgggg ggccgcctcc agcatgtcca ggaggagcgc cgtgagcgcg
    14821 tgcgccatgg cgtcacgatc tccggcgacc gccatcggca cgtcggccag ggtcgccttc
    14881 agccgcatcc tccggtaccg cgcgtaggag tcgaacacga tcgcgagctc ctcgcacagc
    14941 gtgcggaaat cgaaccgcgt gcccggggtt tcggagagcc gcatctgccc gaccaccgcc
    15001 ttcagcatcc ggtcgagcgg cgggatctcg tcggcgacga gagccgcgaa acggcggatg
    15061 cgggccatgg tctggggatc gggatcctcc ttggcggcgg cgcgttggag cagctcgaga
    15121 ttcagcaccc tcgtattcag gaacgcatgc aggtcgtgcg ccagcaccgg gtacatccgc
    15181 ccgaggttcc ggaggtggcg cgcgagggtc gcgtcgtctc ccggcgtcag cggatcgggc
    15241 atggcggttc cggcgcggga atcggcgttt tcctggtcgt gaacgtgcag gggcgttcct
    15301 ccttcggctg cgactccgtc ctcccgggtc agggtggccg gatcggtccg gcggggccgg
    15361 atgcgggcgc acccggagcc gcccgcccaa cgcggagctg cctctaagct ctaagtttga
    15421 gggtccgggg cggccgattc aagcaagggt ttgatcccct tggtttccat ggccttgtcc
    15481 ttgcacgaat cggtggcatg cggaagcgcg ccgaaccgaa gtcgtgtcgt gcgcgtgccg
    15541 cgtcgggatt tcgcgagtcg ccggttcgga acgcggcagg agccgcgccc caacccgtac
    15601 ggtcattgta acaacgccgc cgcaactcgc gaccgatcat cccgcgacag ccacgcacca
    15661 cccgattgga ggcagccatg tacgaactgc agcagtcgag cgt
//
LOCUS       DASSLZ010000036         9062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_14315, whole genome shotgun sequence.
ACCESSION   DASSLZ010000036 DASSLZ010000000
VERSION     DASSLZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9062
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14315"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="VFM00_01280"
     CDS             complement(<1..1704)
                     /locus_tag="VFM00_01280"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET7496907.1"
                     /translation="MSLAALAPAAMAQETGEHHGGEANLQLPDLSSVTFAGGVNGHTL
                     LLFGLAICALGLLFGILTYGQLKNLPVHQSMREMSELIYETCKTYLTQQGKFLIILWV
                     FISLIVLVYFKWLAVTGVDAAGAMVRGFPAFKVAVILFFSLVGMAGSYSVAWFGIRIN
                     TFANSRTAFASLRGKPFPCYSIPLKAGMSIGMALISVELLIMLIILLFVPGDMAGPCF
                     IGFAIGESLGAAALRIAGGIFTKIADIGSDLMKIVFKIKEDDARNPGVIADCTGDNAG
                     DSVGPSADGFETYGVTGVALISFILLAIHDPVVQVQLLVWIFVMRVMMIVASGASYLI
                     NEAWSKAQYENADHMDFEAPLTRLVWLTSFVSIIATYAASYVLIRGLGDGSLWWKLST
                     VITCGTLAGAVIPELVKIFTSMNSKHVAEVVTASREGGASLNILSGLVAGNFSAYWLG
                     MAILMLMAIAYGVSTLGLASIMIAPAVFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQ
                     SIYELSLIETTPGIQAQLRSQYGFDVDFARAKDNLEENDGAGNTFKATAKPVLIGTAV
                     VG"
     gene            2219..4192
                     /locus_tag="VFM00_01285"
     CDS             2219..4192
                     /locus_tag="VFM00_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496908.1"
                     /translation="MEPADGDCFECHSDETATGTKNGKTISIYVDAKKFKSFAHGSVP
                     CVGCHADLRGKDLPHDEELAPVQCGSCHGTEQAQHAKSLHGQAIARGDPLAPRCQSCH
                     GPAHAILSRKDTRSPVAPLQVPYTCGKCHQEGSPVQKQRTIHQDHIVENFSESIHGEA
                     LLRKGLVVAPNCASCHTAHSILPHTDAESSIARRNIAKTCTQCHAEIEVVHRKVIRGE
                     LWEKQAHVLPACIDCHQPHKIRKVFYDQGMADRDCMRCHSEPGLKASGDGRSLFVNAD
                     HLAGSRHAKISCSQCHSDVTASHLRPCETIKQKVDCSSCHAEVGRQYVESVHGTLFAK
                     KDANAPTCKECHGTHEILGKADPDSPTYPTSIPALCARCHREGEKAARRYKGTQHDIV
                     QHYTESIHGKGLMKSGLTVTATCTSCHTAHGELPKDDPVSSVNRANIPHTCGRCHHGI
                     EEQFAQSVHATRVGHTKKDLPVCSDCHTAHTISRTDATGFKLDIMTKCGRCHEDIARS
                     YWDTYHGKVSQLGYTKTAKCYDCHGAHDILPVTDPHSHLSRQNVVATCRKCHEGATRR
                     FAGYLTHATHHDPKKYPFLFWTFWGMTSLLIGTFTVGGLHTLLWLPRALKERKKRNGH
                     RTEEGIEPDPPASADGTGPDTAKDEDTGGSHEG"
     gene            4182..5108
                     /locus_tag="VFM00_01290"
     CDS             4182..5108
                     /locus_tag="VFM00_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496909.1"
                     /translation="MKAEVEPQAQNPGPVTAESADPRAGFGIQPFARPEVQFQRFTRL
                     NRMLHVTMIVSFISLATTGLTLKFSFTGWARILSRLLGGFETAGYIHRFAAMLMIAAF
                     LTHLVDLYRRKRQEYGSWRRLIFGPDTMLLTKRDLGEFVGSIRWFLGRGPRPRYGRWT
                     YWEKFDYFAVFWGMMIIGSTGLMLWFPELFTTVLPGWLINVATIIHSDEALLATGFIF
                     TVHFFNTHLRPEKFPMDLVIFTGRMPLEEFREDKPSEYEALVASGQLRNHLVEAYPPI
                     VIRAVRAFGWTALTLGFGIVLWIIYAMVFAYR"
     gene            5356..6123
                     /locus_tag="VFM00_01295"
     CDS             5356..6123
                     /locus_tag="VFM00_01295"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET7496910.1"
                     /translation="MKPKVKVHLLGKLSVSLNGADLFARAPQKTRELFAFLALHPGRE
                     HPREALATRLWSGIEEPRARKNLRQTIWHLRRSLKPLGPARARRVLRAEPGWLRLEAA
                     EDLWTDVSELRSALARFAHADPASESEEVLRSAASLYRGDLLEGWHQDWCADPREFCR
                     DAYLALLDRLIEAREAAGDLEAGIEYAMRALQFDRARERTHRALMRLFVANGDRTGAL
                     RQYDRCVAAVREELSARPEEETVALMREIRSGKKLRA"
     gene            complement(6120..6488)
                     /locus_tag="VFM00_01300"
     CDS             complement(6120..6488)
                     /locus_tag="VFM00_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7496911.1"
                     /translation="MGKPVVHWEFWSTDPAGVSDFYAKVFDWKIQNIPELNYRFVETG
                     GGAGINGGIMQPDRSGPWPGNMAFYIDVEDLATYRKRIVDAGGKILVEEQQVPGMGSF
                     SLFADPEGRVLGIWKQTAPR"
     gene            complement(6548..7804)
                     /locus_tag="VFM00_01305"
     CDS             complement(6548..7804)
                     /locus_tag="VFM00_01305"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7496912.1"
                     /translation="MFLTAAEAAEVRERFGTPCYVYDRVAAEEAADRALAFPNAFGLT
                     VRYAMKANPNRALLEIFRDKGLDIDASSDFEVERALRAGFLPGRIMLTSQVPSPRLDR
                     WIPLGVLYNACSLHQLEGYGRAFPGRDVTIRVNPGQGSGASHRVNTGGPTSSFGIWHE
                     YLDQAKELAAAHGVVIRGLHTHIGSGSDPAVWTRVAHLVLEIALLLPDVRTVSLGGGF
                     KVARVQGESETDLHAVGETARRAVIDFEKRTGRALRLEIEPGTYLVANAGVVVATCVD
                     VVDTGAKGFLFAKLDTGMTEVMRASLYGAQHPIGVAAAESRPRQAVVFVGRCCETGDM
                     LTPAPGDTTKLAPREVPRPEIGDLVLVGGAGAYCAAMSTLNYNSYPAAPEVLRERDGT
                     LRLVRRRQTIEEMTADELPGSGGAPA"
     gene            complement(7836..8639)
                     /locus_tag="VFM00_01310"
     CDS             complement(7836..8639)
                     /locus_tag="VFM00_01310"
                     /inference="COORDINATES: protein motif:HMM:NF033196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7496913.1"
                     /translation="MPMPRPQPFRFAVHLVVIVPAALLVLLAPASPTVSAQQAQFEWP
                     AHPKNLKVLPKGTTPDQLRDTMVGFTQALGVRCPYCHVGEEGKPLTTFDFSSDAKPTK
                     QIARDMVRMMGDIHKDLGKMKLSPDVPRVNLRCATCHHGHARPTTLAEELMVTYDRAG
                     IDSTLAAYQRLKGRYFGRDAYDFSENGLADFGTALQKKGRADDAIRVHQLNVQENPAS
                     MRAYGALEQAYEAAGKKQEAIDTYHKMLDMDPSNANIQRRLHELEGSGK"
     gene            complement(8693..>9062)
                     /locus_tag="VFM00_01315"
     CDS             complement(8693..>9062)
                     /locus_tag="VFM00_01315"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HET7496914.1"
                     /translation="IRHAVRVNGLTQLVITKLDVLDSLEEIAVVTGYMVGGRKLAEMP
                     ESLTELIAAKPVLETFPGWKTATGGAREWDDLPPEARRYLDRLEMLARVPIRLVSVGS
                     GRDENVRRHANLRSTETVGL"
BASE COUNT         1525 a         3147 c         2994 g         1396 t
ORIGIN      
        1 gccgaccacg gcggttccga tgagcaccgg cttggcggtc gccttgaagg tgttccccgc
       61 cccgtcgttc tcctccaggt tgtccttcgc ccgcgcgaag tcgacgtcga atccgtactg
      121 cgacctgagc tgcgcctgga tgccgggcgt cgtctcgatc agcgagagct cgtagatcga
      181 ctgcgcgttg tcggtgaccg gcccgtacga gtccacggcg atcgtcaccg ggcccatccc
      241 caggaagccg aacgccacga ggccgaacgc gaacacggct ggcgcgatca tgatcgacgc
      301 aaggcccagc gtgctcacgc cgtaggcgat cgccatgagc atgaggatgg ccatccccag
      361 ccagtaggcg ctgaagttgc ccgccacgag gcccgagagg atgttcagcg aggcgccgcc
      421 ctcgcgcgag gcggtgacca cctcggcgac gtgcttggaa ttcatcgacg tgaagatctt
      481 gaccagctcc gggatcaccg cgccggcgag cgtgccgcag gtgatcacgg tggagagctt
      541 ccaccagagc gatccgtcgc cgaggccgcg gatcaggacg tacgaggcgg cgtacgtggc
      601 gatgatggag acgaaggagg tcagccagac gaggcgcgtg agcggcgcct cgaagtccat
      661 gtggtcggcg ttctcgtact gcgccttgct ccacgcctcg ttgatgaggt aggaagcgcc
      721 gctcgccacg atcatcatga cgcgcatcac gaagatccag accaggagct gcacctggac
      781 gaccggatcg tggatggcga ggaggatgaa ggagatgagc gcgacgccgg tcacgccgta
      841 cgtctcgaag ccgtccgcgg aggggccgac cgagtcgccg gcgttgtcgc ccgtgcagtc
      901 ggcgatcacg cccgggttcc gcgcgtcgtc ttccttgatc ttgaagacga tcttcatgag
      961 gtcggagccg atgtcggcga tcttcgtgaa gattccgccc gcgatacgga gcgcggccgc
     1021 gcccagcgac tcgccgatgg cgaagccgat gaagcagggg ccggccatgt cgccgggcac
     1081 gaacaggagg atgatgagca tgatcaacag ctcgacggag atcagcgcca tgccgatgct
     1141 catccccgcc ttgagcggaa tcgagtagca ggggaagggc tttccgcgaa gggaggcgaa
     1201 cgcggtgcgc gagttggcga acgtgttgat ccggatcccg aaccaggcga ccgagtagct
     1261 ccccgccatg cccacgaggc tgaagaagag gatcaccgcg accttgaagg ccgggaatcc
     1321 acggaccatc gccccggccg cgtccacgcc cgtcacggcg agccacttga agtagacgag
     1381 gacgatgagg ctgatgaaga cccataggat gatgaggaac ttgccctgct gggtcaggta
     1441 ggtcttgcac gtctcgtaga tcagctccga catctcccgc atcgactggt gcaccgggag
     1501 gttcttgagc tgaccgtagg tcaggatgcc gaacaggagg ccaagcgcgc agatggccag
     1561 gccgaagagg aggagcgtgt gtccgttcac ccccccggca aaggtcacgg agctcaagtc
     1621 gggcagctgg aggttcgcct cgccgccgtg atgctctccg gtctcctgcg ccatcgcggc
     1681 cggcgcgagc gccgcgagac tcatggcgca gagcgccgcg agcacgatcg ccaggaccgt
     1741 tccgcgcact cccgtcgtct tccacttcat catgtcgtac ctcacctcgt gttgagactg
     1801 ggtggggttg gccgcctggg gggttggcag acgcttcatc gcccaaaggt aggaacgaaa
     1861 acccccgaga agtcgaagat gtatagcagg ttggcctcca tctgtcgaga gggtagccat
     1921 ggaccggccg agggactact acctacggcc acctgacgtc gcgcccagcg gtccgtagtt
     1981 tccacccttc catgtgccgc agattcaagg tgtgaggccg aactctcgaa tggatcgacg
     2041 cggctccgtc cggagacccg ctctccgctc caaatcgttg tggttccggg tattcgcggc
     2101 ccaggcgctc gtttggggtc tggcggcggt cgcgtggccg gccggatccg ccctggccca
     2161 ggcgcaggcc caggccggcg ccccccccgc cgccgctccc gcggtcaagg cgccagccat
     2221 ggagcccgcc gacggcgact gcttcgagtg ccactccgac gagaccgcga ccggcacgaa
     2281 gaacgggaag acgatctcca tctacgtcga cgcgaagaag ttcaagtcgt tcgcgcacgg
     2341 gtcggttccc tgcgtcggct gccatgccga tctccgcgga aaggacctcc cgcacgacga
     2401 ggagctggcc ccggtccagt gcggctcctg ccacgggacg gaacaggcgc agcacgcgaa
     2461 gtcgctccac ggccaggcga tcgcccgcgg cgatcccttg gcgccccgct gccagagctg
     2521 ccacggcccc gcgcacgcga tcctttcgcg caaggacacg cgctccccgg tggctccgct
     2581 gcaggtgcca tatacctgcg gcaagtgcca ccaggaaggg tcgccggtcc agaaacagcg
     2641 cacgatccac caggatcaca tcgtcgagaa cttctccgag agcatccacg gcgaagccct
     2701 cctccggaag gggctcgtgg tcgcgcccaa ctgcgcctcc tgccacacgg cccactcgat
     2761 cctgccgcac acggacgccg agtcctcgat cgcgcgtcgc aacatcgcca agacctgcac
     2821 gcagtgccac gccgagatcg aggtcgtgca ccgcaaggtg atccgcggcg agttgtggga
     2881 gaagcaggcg cacgtgctcc ccgcctgcat cgactgccac cagccgcaca agatccggaa
     2941 ggtcttctac gaccagggca tggcggacag ggactgcatg cggtgccatt ccgagcccgg
     3001 gctcaaggcg agcggagacg gccggtccct gttcgtgaac gccgaccacc tcgccggttc
     3061 gcgccacgcc aagatctcct gcagccagtg ccactcggac gtcacggcgt cgcacctccg
     3121 cccctgcgag acgatcaagc agaaggtgga ctgctcctcc tgccacgccg aggtgggccg
     3181 ccagtacgtg gagagcgtgc acggcaccct cttcgcgaag aaggacgcga acgcgccgac
     3241 gtgcaaggag tgccacggca cccacgaaat cctgggcaag gccgatcccg actcgcccac
     3301 ctaccccacc agcattcccg ccctctgcgc gcgctgccac cgcgaggggg agaaggcggc
     3361 gcgccgctac aaggggacgc agcacgacat cgtgcagcac tacaccgaga gcatccacgg
     3421 gaaggggctc atgaagagcg gcctcaccgt gaccgcgacc tgcaccagct gccacacggc
     3481 gcacggcgag ctgcccaagg acgatccagt ctccagcgtg aaccgcgcga acatcccgca
     3541 cacctgcgga cggtgccacc acggcatcga ggagcagttc gcccagagcg tccacgccac
     3601 ccgcgtgggc cacacgaaga aggatcttcc cgtctgcagc gactgccaca cggcgcacac
     3661 gatcagccgc accgacgcga cggggttcaa gctggacatc atgaccaagt gcgggcgctg
     3721 ccatgaggac atcgccaggt cctactggga cacctatcac ggcaaggtct cgcagctggg
     3781 ctacacgaag acggccaagt gttacgactg ccacggcgcg cacgacatcc tgcccgtgac
     3841 cgacccgcac tctcacttaa gccgccaaaa cgtcgtggcg acctgccgga agtgccacga
     3901 gggcgccacg cgccgcttcg cgggatatct gacccacgcg acgcaccacg accccaagaa
     3961 gtatcccttc ctcttctgga ccttctgggg aatgacgtcg ctcctgatcg ggaccttcac
     4021 ggtgggcggc ctccatacgc tgctctggct gccgcgcgcc ctgaaggagc ggaagaagcg
     4081 gaacggacac cggaccgagg agggcatcga accggacccg cccgcgtccg cggacggcac
     4141 cggacccgac acggcgaagg acgaggacac cggaggctcc catgaaggct gaggtagagc
     4201 cccaggccca gaacccgggg cccgtcacgg cggagagcgc cgacccgcgc gccgggttcg
     4261 ggatccagcc cttcgcgcgc cccgaggtcc agttccagcg cttcacgcgg ctcaaccgga
     4321 tgctccacgt gaccatgatc gtcagcttca tcagcctcgc gaccacgggc ctgacgctga
     4381 agttctcctt cacgggatgg gcgcggatcc tctcgcgcct tttgggcggc ttcgagaccg
     4441 cgggctacat ccaccggttc gccgcgatgc tcatgatcgc cgcgttcctc acgcacctgg
     4501 tcgacctcta ccggcggaag cggcaggagt acggctcgtg gcggcgcctg atcttcggcc
     4561 ccgacacgat gctcctcacg aagcgggacc tgggcgagtt cgtggggagc atccgctggt
     4621 tcctgggccg gggaccgcgc ccgcgctacg gccgctggac ctactgggag aagttcgact
     4681 acttcgcggt cttctggggc atgatgatca tcgggtcgac cgggctcatg ctctggttcc
     4741 ccgagctctt cacgaccgtc ctgcccggct ggctcatcaa cgtggccacg atcatccaca
     4801 gcgacgaggc gctgctcgcg accggattca tcttcacggt gcacttcttc aacacgcacc
     4861 tccgccccga gaaattcccg atggacctcg tgatcttcac cggccggatg cccctcgagg
     4921 agttccgcga ggacaagccg tccgagtacg aagcgctcgt ggcgagcggc cagcttcgga
     4981 accatctcgt cgaggcctac ccgccgatcg tgatccgcgc cgtacgcgcg ttcggctgga
     5041 cggccctcac cctgggcttc ggcatcgtgc tctggatcat ctacgcgatg gtgtttgctt
     5101 atcgttaaac tgccggctct gccggcagag gctgtcaaca acagacgaca aacctcagcg
     5161 tcaaatagga cacacagcta gcgcctgcca gctgcccaaa gcggcacccc gcacccctcc
     5221 cccgccctaa ctctcgattc gatcacacca cgcccccgac aaccctcctg aaagcgtccg
     5281 gcacggatcc tgtttacccg tgaggcgaag tctgttcacg gagctacgca gcgacccatg
     5341 gccatgagac cccccatgaa gccaaaggtc aaagttcacc tattgggcaa gctttccgtc
     5401 tcgctgaacg gggcggatct gttcgctcgc gcgccgcaga aaacgcgcga gctgttcgcg
     5461 ttcttagcgc tccaccccgg gcgcgagcac ccccgcgaag cgctggccac gcgtctctgg
     5521 agcggcatcg aagagccgcg cgctcggaag aacctgcgcc agaccatctg gcacctgcgc
     5581 cggagcctca agccgctggg ccccgcgcgg gcgcgtcgcg tgcttcgcgc cgagcccgga
     5641 tggctgcggc tggaggcggc cgaggacctc tggaccgacg tgagcgagct gcgttcggcg
     5701 ctcgcgcgct tcgcgcacgc ggatcccgcc tcggagtcgg aggaggtgct gcggtcggcg
     5761 gcgagcctgt atcgcggcga tctcttggag ggatggcacc aggactggtg cgccgacccg
     5821 cgggaattct gccgggacgc ctacctcgcg ctgctggacc ggctgatcga ggcgcgcgag
     5881 gcggcgggcg acctggaagc ggggatcgag tacgcgatgc gcgcgctcca atttgaccgc
     5941 gcccgcgaac ggacccaccg cgcgctgatg cgcctcttcg tggcgaacgg ggaccgcacg
     6001 ggcgcgctgc gccagtacga ccgctgcgtg gccgcggtgc gcgaggagct gagcgcgcgc
     6061 cccgaggagg agacggtggc gctgatgcgc gagatccgct ccgggaagaa gctccgcgct
     6121 tagcgcggag cggtctgctt ccagatcccg agcacccgac cctcggggtc ggcgaagagc
     6181 gagaaggaac ccatcccggg aacctgctgc tcttccacga gaatcttccc gcccgcgtcc
     6241 acgatccgct tccggtaggt cgcgaggtcc tccacgtcga tgtagaaggc catgttcccg
     6301 ggccaggggc cgctccggtc gggctgcatg atgccgccgt tgatgccggc gccgccgccc
     6361 gtctcgacga agcggtagtt cagttcgggg atgttctgga tcttccagtc gaagaccttc
     6421 gcatagaagt cggagacccc ggccggatcg gtggaccaga attcccagtg gaccacgggc
     6481 tttcccatga cgcgctcctt tccgtagggg ccaggccgcg cggccgaccc cgacgcacga
     6541 taaccagcta ggccggcgcg ccgccgctgc ccggcagctc gtcggccgtc atctcctcga
     6601 tggtctgcct ccgccggacc aggcggaggg ttccgtcgcg ctcgcgcaac acctcgggcg
     6661 ccgcgggata cgagttgtag ttgagcgtcg acatcgccgc gcaataggcg ccggcgcctc
     6721 cgaccaggac cagatctccg atctcggggc gcggcacttc gcgcggagcg agcttcgtcg
     6781 tgtcgcccgg cgccggcgta agcatgtccc ccgtctcgca gcagcgcccc acgaacacga
     6841 ccgcctggcg cggccgcgac tcggccgccg cgaccccgat cggatgctgg gcgccataga
     6901 gcgaggcccg catcacttcg gtcatgccgg tgtccagctt ggcgaacagg aagcccttcg
     6961 ctcccgtgtc caccacgtcc acgcaggtcg ccaccaccac ccccgcgttg gccacgaggt
     7021 aggtgccggg ctcgatctcc agacgcagcg cgcgcccggt tcgtttctcg aaatcgatca
     7081 ccgcccggcg cgcggtctcc cccacggcgt ggaggtcggt ctccgactcg ccctgcaccc
     7141 gcgccacctt gaagccaccg cccagactga ccgtgcggac gtccgggagc aggagggcga
     7201 tctcgagcac gagatgcgcg acgcgcgtcc acaccgcggg gtcgctcccc gagccgatgt
     7261 gggtatggag gccgcggatg acgacgccat gcgccgcggc cagctccttc gcctggtcca
     7321 ggtactcgtg ccagatgccg aagctggacg tgggcccgcc ggtgttgacc cggtgcgagg
     7381 cgccgcttcc ctgtcccggg ttcacccgga tcgtgacgtc ccgaccgggg aaggcccttc
     7441 cgtagccctc gagctggtgg agcgagcagg cgttgtagag gacgccgagc ggaatccacc
     7501 ggtccagccg gggcgagggc acctgcgagg tcagcatgat gcgccccggt agaaacccgg
     7561 cgcggagcgc gcgctcgacc tcgaagtcgc tcgaggcgtc gatgtcgagc cccttgtcgc
     7621 ggaagatctc gagaagcgcc cggttggggt tggccttcat cgcgtagcgg acggtgagcc
     7681 cgaacgcgtt ggggaacgcg agcgcgcggt cggcggcctc ttcggccgcg accctgtcgt
     7741 aaacatagca gggagtgccg aaccgctcgc gaacctcggc ggcttcggcc gcggtcagga
     7801 acaggtcgac actccctcgt cgcggaagcc ccgggctact tcccggagcc ttccagctcg
     7861 tggagccggc gctggatgtt ggcgttgctc ggatccatgt cgagcatctt gtggtaggtg
     7921 tcgatcgcct cctgcttctt cccggccgct tcgtaggcct gctccagcgc gccgtacgcg
     7981 cgcatcgacg cggggttctc ctgcacgttc agctggtgca cccggatcgc gtcgtcggcc
     8041 cgccccttct tctggagcgc cgtgccgaag tccgccagtc cgttctcgct gaagtcgtag
     8101 gcgtcgcgtc cgaagtaacg ccccttgaga cgctggtagg ccgccagggt ggagtcgatc
     8161 ccggcccggt cgtaggtcac catcagctct tcggcgagcg tcgtggggcg cgcgtggccg
     8221 tggtggcagg tggcgcagcg caggttcacg cgcggcacgt ccggcgagag cttcatcttg
     8281 ccgaggtcct tgtggatgtc gcccatcatc cgcaccatgt cgcgcgcgat ctgcttcgtg
     8341 ggcttggcgt cggacgagaa atcgaacgtg gtgagcggct tcccctcttc gccgacgtga
     8401 cagtagggac aacgcacccc cagcgcctgg gtgaacccga ccatcgtgtc gcggagctgg
     8461 tcgggcgtcg tgcccttggg caggaccttg agattcttcg ggtgggcggg ccattcgaac
     8521 tgcgcctgct gggcgctgac cgtgggcgac gccggcgcga gcagcaccag cagcgcggcc
     8581 ggaacgatca cgacgaggtg cacggcgaag cggaagggct gcgggcgagg catcggcatc
     8641 gttcctctcc ggcgaggcgg ggggacgtac ggaatcggtt ctcgagcggc tcctacagtc
     8701 cgaccgtctc ggtggagcga aggttggcgt ggcggcgcac gttttcatcg cgccccgagc
     8761 ccaccgagac gaggcgtatc gggacccggg ccagcatctc caggcggtcg aggtagcggc
     8821 gcgcctcggg cgggaggtcg tcccattccc gcgccccgcc ggtcgccgtc ttccatcccg
     8881 ggaaggtttc gagcacgggc ttggccgcga tgagctcggt cagcgactcc ggcatctcgg
     8941 ccagcttccg tccgccgacc atataccccg tgaccaccgc gatctcctcc agggagtcga
     9001 gcacgtcgag cttggtgatg acgagctgcg tgagcccgtt cacgcgcacg gcgtggcgga
     9061 tc
//
LOCUS       DASSLZ010000037        15504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_141451, whole genome shotgun sequence.
ACCESSION   DASSLZ010000037 DASSLZ010000000
VERSION     DASSLZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 15504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15504
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141451"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(371..1819)
                     /locus_tag="VFM00_01320"
     CDS             complement(371..1819)
                     /locus_tag="VFM00_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7496915.1"
                     /translation="MNARTRRLLGAAAIVAAAATNVFAPAPAIADDPTGWIAPGGGEL
                     WIPNEFGVEPHRPMFGAIAGVALSRSLAIEARGIYAKSESDAGATLKLLHGSGDFTLF
                     LLHDRIRPYLMAGAGVMHLDGDSTSATKLALDGGLGFHIRLSDRIALRLDGREVAYKV
                     FVPGENEEKYKHGPEAFASLSFGFGGTSKDSDRDGVRDKTDRCPGTPLGARVDATGCP
                     VDSDQDGVPDGIDRCEGTPKGAAVDATGCTMDADQDGVPDGVDQCSNTPAGARVNANG
                     CPMDTDNDTVPDGLDQCDGTPAGCVVNPNGCPTDADQDGVCDGMDKCPDTPENARVDA
                     TGCPITVSSKETELLETGMIRLQNVNFDTGKAKILPESFATLDEVADILGRWPDLRIE
                     IAGHTDSRGTDARNLALSQARASAVRDYLVNKFPELRPEQFTAKGYGETQPIASNTTT
                     LGRARNRRVEFRVLNKETLRKESEKRGLAPKE"
     gene            1965..3503
                     /locus_tag="VFM00_01325"
     CDS             1965..3503
                     /locus_tag="VFM00_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019887713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET7496916.1"
                     /translation="MAADFKDAIAKGYTFSGESITLGAAMKDGAVVDGAVVRVPLAMM
                     NRHGLVAGATGTGKTKTLQLMAEQLSAQSVPVFLADLKGDLSGIAAPGESSPKVLERA
                     KAVGVTFQPAAFPAEFVGLSGKNGVPLRATVSSFGPLLLARAFQLNETQQSVLAMVFK
                     LCDDQKLLLLDLADLKAVLQYLSGEGAGELAAYGGLSKATVGVLLRKIVEMEQQGADR
                     FFGEPEFEVHDLLRVDPSGKGVVTLLELDDIQDRPALFSTFLMWLLASLYRELPEVGD
                     IAKPKLVFFFDEAHFLFEDATKAFLSEVEQTVRMIRSKGVGVFFVTQNPKDVPDGVLA
                     QLGNRVQHALRAFTPDDDKALKAAANTFPRSEFYDIAKTILSLGTGEALVTVLSERGT
                     PTPVAATRMIPPSSLMAPIPPGERERRIQQSPLMAEYGKPVDRESAKEMLEARAQAAP
                     GGAAPASPARRGGRAEPEAPNMAEAVGKAINSPLGRAVGRELVRGLFGVLGVSSGTRR
                     RRSF"
     gene            3514..4011
                     /locus_tag="VFM00_01330"
     CDS             3514..4011
                     /locus_tag="VFM00_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7496917.1"
                     /translation="MYPDAVNAAPGQVRIRSLSELDISRVVAIDERLTGIYRPDVWER
                     RVGYYIRRDADACPVAEADGKVVGFMFSDVRGGEFGLEETSGWIERFGVDPDYQGRSI
                     GRRLFDAVVDHMKQRGATVLRTIVDQKDEELASFLRAVGFKNAPLTSLELPLAGDGKG
                     QAARA"
     gene            4008..5369
                     /locus_tag="VFM00_01335"
     CDS             4008..5369
                     /locus_tag="VFM00_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HET7496918.1"
                     /translation="MNVEETTLPKKFHDAVLHWQRHNFPDYDLLPQYWEKYFPNEPEF
                     CLCAKMELGMSDTIEVGDKKGERRMQAPSEMGEDAAAHLLAIIRAQASTEFGSIQQHE
                     ATLARAQDDEDRFWVLRVMAEELRHGYQMFHMLTSQDWSSVSSQKAEEMVEEILSMRT
                     GSHVLDAFNLDYDSFVDNVVFAAVIDRVGKYQLSMQRVCAYKPFAQSMPPMLREEAFH
                     LAAGVIPLRRWVQRAAQGNELVTMASIQKAFNKWLPRGLEMFGHEKGGDSNVRFGFKN
                     MKNAEAQSQYYDECRKMIADLNQRYVRARSPQLSPEQAETLVARLQKGGGREKGIASH
                     DLLELPDPRFFRRKGEPAWTMIGTRGEAFDTVDAYLAHLKEHLPEPYLASRDLSVYVA
                     TLRDVAAGTVAPEQASKRMPSLKRVGGVCPCSKSVRWIVEEPHAAPAAGSPAPPRTAS
                     GAA"
     gene            5562..6092
                     /locus_tag="VFM00_01340"
     CDS             5562..6092
                     /locus_tag="VFM00_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HET7496919.1"
                     /translation="MVPLNVRGRVLSARRRVLCVLTVVLALFAGCAPAAKQESVTEVR
                     SEIEASNKQFMDAFARRDAAAIGLLYAEDGQVLPPGAPPLDGREAIVTMWQSVLALPF
                     ASVTLQTSDVGVDEESAWETGRYTMTGNDGKTAETGKYIVVWKHGEAGWKIYRDIWNS
                     NTPATSPAPGGTPAAP"
     gene            6011..7696
                     /locus_tag="VFM00_01345"
     CDS             6011..7696
                     /locus_tag="VFM00_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7496920.1"
                     /translation="MEDLSRYLEQQHPRDLARARGDAGRSVAPRLAERRGLVSHAAGE
                     AAWEWDLESGRIQRGGAMLAITGYGPEEIGFDPAWWKERIHPEDRASVLSAIDAAMVG
                     ERRELVMLYRFRRKDGSYALLLDRAFVLGDDPEQPARIVGLAREVTPPVIPPERAPSP
                     VAVEPEEDPPEAGTPSRRLHDSALAFDRITDPALACLPLDVLLSELLDRVRGALRGDA
                     ALALLYSEDGALLECRATAGIQAGASGRFSVPARRGVVARVASSGAPILTNDPQAAGG
                     TPPGWVRLCRSFAGAPIVLEARVVGVLCVVARARLEFDEDDRRLLELAADRLGPAFDR
                     ARLLESERADRDRLASLSRTIVSSLEEERRRVSRELHDEIGQLLTSLRLTLRSSAPSR
                     VSVDEIMDELFARVRDISTRLRPPMLEDLGLGPALSWHCERFALQTGVRVHLMTTGLE
                     RRYPAKAELAVFRVVQEALTNVARHAKVVDARVAVRGTPGTISVLVMDQGVGFPAGSL
                     HEHQSSGLIGMRERARSVGGRLLVSSSPGGGTRVSLRIPIPKGETSPNDEVSS"
     gene            7693..8382
                     /locus_tag="VFM00_01350"
     CDS             7693..8382
                     /locus_tag="VFM00_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7496921.1"
                     /translation="MNTQPITIILADDHRVVREALRSLLESEPDFQVVAEASDGLAAA
                     ELTERQKPDVLVLDLQMPGLNGLDVLRRVVRRSPRTRVVVLSMHAAEGYVVAALKHGA
                     SAYVTKDASASELGIAIRKAIAGQRYVSSPFSSAAVEEYAARSEGAAPDPYETLTGRE
                     REVFHLAAEGRSSAQIAERLGISPRTAETHRANALKKLKLHGQTNLVLYAVEKGLLPS
                     GAPEELRAPRP"
     gene            8433..10430
                     /locus_tag="VFM00_01355"
     CDS             8433..10430
                     /locus_tag="VFM00_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7496922.1"
                     /translation="MTERKRTDTETEKKRVESEELEKTIRELRDSEERYRRLVELSPD
                     GVFVQSEGAIVFANLAGAKLIGADRPQKLFGTPMLELFHPDHRSDTRARLEEAGPKAP
                     AAPFEEKMVRLDGAVIDVEVAAAPFTYQGKPSTQIMIRDITSRKESEETLRRQAEFIR
                     AVAESTGEGLYAIDRAGRVTFMNPAAGEMMGWTREELFLRDLHQMTHYRRPDGTSFPR
                     HECPILTAMENRTTIERDDVFIRKDGSMLAVSLSSSPLRSANGETVGAVILFRDATER
                     RRLEEQLLHAQKLEAVGRLAGGIAHDFNNLLSVIAGYGELVLADLKEPGAARSRVLEI
                     LRATERATGLTRQLLAFGRKQVIEPRVLDLNATAQDAERMLRRLIGEDIDIAMKLAPD
                     LGRIQADPGQVEQVIINLAVNARDAMPRGGKLTIETTNVALDDDAVRGYVDVKPGHYV
                     RLCVSDTGVGMDRETREHLFEPFFTTKEAGRGTGLGLATVYGIVKQSGGHVWVYSEPG
                     WGTTFKVYFPRFGDVAEPGRPAIEAGPAPGGTETILVVEDDEMIRSLIRDILESTGYR
                     VLVADDPESGMKLIGEEQEIHLLLTDLILPGMSGRELVDKVVELKPDIRVLFMSGYSD
                     EAVARHGILEPGLAFLQKPFSRDALVRKVREVLDSAGSRIA"
     gene            10487..10972
                     /locus_tag="VFM00_01360"
     CDS             10487..10972
                     /locus_tag="VFM00_01360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7496923.1"
                     /translation="MRDDDWDEVRRVYAEGIATRQATFETETPAWEAWDRGHRKDCRF
                     VARAPGPSHHRLLGWAALSPVSGRCVYAGVAEVSVYVSEDARGRGVGRILLAALIQAS
                     ERQGIWTLQAGIFPENQASVRLHRALGFREVGKRERIGQLDGAWRDVLFLERRSAIAG
                     V"
     gene            complement(10978..12156)
                     /locus_tag="VFM00_01365"
     CDS             complement(10978..12156)
                     /locus_tag="VFM00_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018893396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7496924.1"
                     /translation="MRLRAAALLLLLFATASCGGNSREVPPPPAASTADYHAVLVTSG
                     LSQPVGAVAAPDDTARLFILEKTGAIRILQGGALRPRPFLDLSARVSGGSEQGLLGLA
                     FHPGYAANGRFFVDYTDLSGDTRVVEFQVSANPDSAGATEKEILFVDQPFANHNGGQL
                     AFGPDGKLYVGLGDGGSGGDPQGNGQNLDALLGKILRLDVDAASPYTVPPDNPFVGRA
                     GARGEIWSYGLRNPWRFSFDAETGAMWIGDVGQDSWEEIDVEPAGQGGRNYGWNRMEG
                     THCYPPGLSCNPAGITLPIAEYDHGVGCSVTGGFVYRGMELPELRGTYFYGDYCTGIV
                     RSLRLVSGAPAVPRDWTPVLRTAAGGPMQGLSSFGVDGKGELYLTLLSGEIYRLARRP
                     "
     gene            complement(12422..13108)
                     /locus_tag="VFM00_01370"
     CDS             complement(12422..13108)
                     /locus_tag="VFM00_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496925.1"
                     /translation="MIRTVTMGAAVAALVGAALLATPGDAKADPGGKWWTPKQGRERR
                     VERIYRRDSAPRGFSRQWRSWGDRRVYRDMIVIRASRGPRYQAWRTYCPPEYIYSRHV
                     IRVRPIRFVVAASGVIGGVRFHGSYHDDDYLYGCNFCDARFSSYGAYQTHVNSCSHRP
                     RGYRVECSDWRGSGDWGDDGWRDLGDRGDYCDRGYDRDYDYDDGRYDRGDRCEDDHRG
                     DVDDDDDYDR"
     gene            13416..14003
                     /locus_tag="VFM00_01375"
     CDS             13416..14003
                     /locus_tag="VFM00_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496926.1"
                     /translation="MAAFSDWDSLEYQALMARGRDAERTALVCWTASGIAAAMLLSWG
                     IAAKSASLMLPVIFATAFGFYAAVHGRQQVRLIAGYVKEFMEPNGNSGPQWFTRLAQL
                     EVVPGFNPSGDWAVAALANLTVVTAMAFSWIYAGGAGQRGDLMAGIATGLGLVFCFHS
                     ITETARIRQTNAGALWRQITSHAEERRGPRLASRG"
     gene            complement(14076..>15504)
                     /locus_tag="VFM00_01380"
     CDS             complement(14076..>15504)
                     /locus_tag="VFM00_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7496927.1"
                     /translation="SSRLVFAPYAVKGLDGVERPAELGRLTVPEQHARPAGKQIAIVF
                     LRFRSPSTSPGPPIVFLPGGPGYPGTLLARTPPYLEFFEKLRSGSDVIVLDQRGSGLS
                     DPTLQCAVRGSLPPDAFESEAKTASALGSMLRPCVRLMRNDGIAIEAYNTGESAEDLE
                     DLRRALGVERLRLIGVSYGTELALEMIRRHGDRVDAAVLAGTRGPDMTWRLPSVNDFQ
                     LKRVSTLVAQDAAWGHDLPDLEGSVRRLIERLSWRPATLRITDRKTGKVVGMRIGPSG
                     IQAVLGSDLNDWTRAPFLPALFASLANGDSTLLVRRVEDLVNATASGISVMQVTTDCA
                     SGASPERRGVVARESRTALLGNVKNMLVNPAFCDLVGGADLGPAFRERIYSPVRTLFL
                     TGSMDGITPPFQAEEVRWGFPNGIHLVVENGWHEVLPFNDVQQAVQDFFAGQDVRGRR
                     IELPRPRFLSLAEAKSLTAPAPPRR"
BASE COUNT         2362 a         5393 c         5392 g         2357 t
ORIGIN      
        1 ctgacggcct cgggcgctcc agagacaaca ctccctccgc ggtttccttc ccgctcgcct
       61 ccgactacct cctcgctccc gactaccccc tcgctcccga ctaccccctc gctcccgacg
      121 gatcccctcc ccgactgacc ccgactcacc cccggctacc tcgaccaact tcgaccgacc
      181 gcagacggcc ccgatattca cgtgtcacac gcagaggcca gaaaaaggca gcggggccgt
      241 gcaagaaagc cacggccccg ccttgcccct tcccgatcag gaccgttcct cgcgcgtcgc
      301 cgcgcgattc gcgttccttc accggcccgg agaacccccc tcccatcacc ccgcacacgg
      361 cccgcgggga tcactccttc ggggcgagcc ctcgcttctc cgattccttt cggagcgttt
      421 ccttgttcag tacgcggaac tccacgcgcc ggttgcgggc gcgtcccagc gtcgtcgtgt
      481 tcgacgcgat cggctgcgtc tcgccgtagc ccttcgcggt gaactgctcc ggtcgcagct
      541 cggggaactt gttcacgaga tagtcgcgca ccgcgctggc gcgcgcctgg ctcagggcca
      601 ggttgcgcgc gtcggtgccg cgcgaatcgg tgtgtcccgc gatctcgatg cgcagatccg
      661 gccaccgtcc caggatgtcg gccacctcgt ccagggtggc gaacgactcc ggcaggatct
      721 tcgccttgcc ggtgtcgaag ttcacgttct ggagccggat catgccggtc tcgagcagct
      781 ccgtctcctt ggagctgacc gtgatggggc agccggtggc gtccacgcgc gcgttctccg
      841 gcgtgtcggg gcacttgtcc atcccgtcgc acacgccgtc ctgatcggcg tcggtggggc
      901 agccgttcgg gttcacgacg catccggccg gcgttccgtc gcactgatcg aggccgtccg
      961 gaacggtgtc gttgtcggtg tccatcgggc agccgttggc gttgacgcgt gcgccggccg
     1021 gcgtgttgct gcactggtcg acgccgtcgg ggacgccatc ctgatccgcg tccatcgtgc
     1081 agcccgtcgc gtcgacggcg gcgcccttgg gcgtaccctc gcagcggtcg atgccgtcgg
     1141 ggacgccgtc ctgatcgctg tccaccgggc agccggtcgc gtcgacgcgc gcgccgagcg
     1201 gcgtgccggg gcagcggtcg gtcttgtcgc gcacgccgtc gcgatcgctg tccttggacg
     1261 tgccgccgaa cccgaaggag agcgatgcga aggcttcggg accgtgcttg tacttctcct
     1321 cgttctcgcc gggaacgaac accttgtagg ccacctcgcg tccgtccagg cgcagcgcga
     1381 tgcgatcgct gaggcggatg tggaagccca gtccgccgtc caaggcgagc ttggtcgcgc
     1441 tcgtgctgtc gccatccagg tgcatcacgc cggcccccgc catcagatag ggccggatgc
     1501 ggtcatggag gaggaacagc gtgaagtcgc cgctcccgtg gagcagcttc agggtcgcgc
     1561 cggcgtcgga ttccgacttc gcgtagatgc cgcgcgcttc gatggccagg gaacgcgaga
     1621 gcgccacgcc cgcgatcgcg ccgaacatgg ggcgatgggg ctcgacgccg aactcgttcg
     1681 gaatccagag ctcgccgcct ccgggcgcga tccacccggt gggatcgtcg gcgatcgcgg
     1741 gagctggcgc gaagacgttc gtcgcggcgg cggccacgat cgcggcggcg ccgagcaggc
     1801 ggcgcgtccg tgcgttcacg ggcgggagtc ctccaagtgg gtggtcgcgc ggcgcgggcg
     1861 tacatgccgc gcggcccgcg cactctaacg aacgcgccgc gccaggcgca agcgcgatgt
     1921 gtcgcgcgat tgacgcgcgc gcggatcgct ggcagcatcg cggcatggct gcggatttca
     1981 aggacgcgat cgcgaagggc tacacgttct cgggcgagtc gatcacgctt ggggcggcga
     2041 tgaaagacgg cgcggtggtc gacggcgcgg tcgtccgcgt gccgctcgcg atgatgaacc
     2101 gccacgggct ggtcgccggc gccaccggca cgggaaagac gaaaacgctc cagctcatgg
     2161 ccgagcagct ctccgcccag agcgtcccgg tgttcctcgc cgacctcaag ggcgacttga
     2221 gcgggatcgc cgctcccggc gaatcgagcc ccaaggtgct cgagcgggcg aaggcggtgg
     2281 gcgtcacgtt ccagcccgcg gcgtttccgg cggagttcgt cggcctctcg ggcaagaacg
     2341 gcgttccgct ccgcgccacg gtctcctcgt tcgggccgct cctcctcgcg cgcgcgtttc
     2401 agctgaacga gacgcagcag agcgtcctgg ccatggtctt caagctgtgc gacgaccaga
     2461 agctcctcct cctcgacctg gccgacttga aggcggtgct ccagtacctc tccggcgagg
     2521 gcgcggggga gctcgcggcg tacggcggcc tctccaaggc gacggtcggc gtcctgctcc
     2581 gaaagatcgt cgagatggag cagcaggggg ccgaccgctt cttcggggag cccgagttcg
     2641 aggtccacga cctgcttcgc gtggatccct cggggaaggg cgtcgtcacg ctcctggagc
     2701 tggacgacat ccaggaccgt ccggcgctct tcagcacctt cctcatgtgg ctgctggcgt
     2761 cgctctaccg cgagctgccc gaggtgggcg acatcgccaa gcccaagctc gtcttcttct
     2821 tcgacgaggc gcacttcctc ttcgaggatg cgaccaaggc gtttctcagc gaggtggagc
     2881 agaccgtgcg gatgatccgg tccaagggcg tcggcgtctt cttcgtgacc cagaacccca
     2941 aggacgtgcc ggacggcgtg ctggcccagc tgggcaaccg cgtccagcac gcgctccgcg
     3001 ccttcacccc cgacgacgac aaggcgctca aggccgcggc gaacacgttt ccccgttccg
     3061 agttctatga catcgcgaag acgattctct ctttggggac cggggaagcc ctcgtcaccg
     3121 tgctctccga gcggggcacg cccacgccgg tggccgccac gcgcatgatc ccgccgtcca
     3181 gcctcatggc cccgatcccg cccggcgaac gggaacggcg gatccagcag tcgccgctca
     3241 tggccgagta cggcaagccc gtcgaccgcg agagcgccaa ggagatgctc gaggcgcgcg
     3301 cgcaggcagc tccgggcggc gccgctccgg cgtcgcccgc ccgtcgcggt ggccgcgccg
     3361 agcccgaagc cccgaacatg gccgaggccg tcggcaaggc catcaattcc cccctgggcc
     3421 gtgccgtggg acgcgagctg gtgcgcggcc tcttcggggt gctcggcgtc tcgagcggaa
     3481 cccggcggcg gcgtagcttc tagcggagcg ccggtgtatc ctgatgcggt gaacgccgcg
     3541 ccggggcagg tccgcatccg ttccctctcc gagttggata tctcccgggt cgtcgcgatc
     3601 gacgaacgtc tgaccgggat ctaccgcccc gacgtctggg agcgccgcgt cgggtattac
     3661 atccggcgcg acgccgacgc ctgcccggtg gccgaagcgg acggcaaggt cgtggggttc
     3721 atgttctcgg acgtgcgtgg cggcgaattc ggcctggaag agacgagcgg gtggatcgag
     3781 cgcttcggcg tcgaccccga ttaccagggg cgctccatcg gccggcggct cttcgacgcc
     3841 gtggtcgacc acatgaaaca gcgcggagcc acggtgctcc gcacgatcgt ggaccagaag
     3901 gacgaggagc tggcctcgtt tctccgggcg gtggggttca agaacgcccc gctaacctcg
     3961 ctcgagctcc cgctggcggg cgatgggaaa ggacaggccg cgcgcgcatg aacgtcgaag
     4021 agaccacgct tcccaagaag ttccacgacg ccgtcctgca ctggcagcgt cacaacttcc
     4081 ccgactacga cctgctgccc cagtactggg agaagtactt tcccaacgag ccggagttct
     4141 gcctctgcgc caagatggag ctgggaatgt ccgacacgat cgaggtcggg gacaagaagg
     4201 gcgagcggcg catgcaggcg ccgtccgaga tgggagagga cgccgcggcg cacctcctcg
     4261 ccatcatccg cgcgcaggcc tccaccgaat tcggctcgat ccagcagcac gaggcgaccc
     4321 tggcgcgcgc ccaggacgat gaagaccgct tctgggtgct ccgcgtcatg gccgaagagc
     4381 tgcgccacgg ctaccagatg ttccacatgc tgacgtcgca ggactggtct tcggtgtcgt
     4441 cccagaaggc ggaggagatg gtcgaggaga tcctctccat gcgcaccgga tcgcacgttc
     4501 tcgacgcctt caacctcgat tacgactcgt tcgtggacaa cgtggtgttc gccgcggtca
     4561 tcgaccgcgt cggcaagtac cagctctcga tgcagcgcgt ctgcgcgtac aagccgttcg
     4621 cgcagtcgat gccgccgatg ctccgcgagg aggcgttcca cctcgcggcg ggcgtcatcc
     4681 cgcttcgccg ctgggtccag cgcgccgccc agggcaacga gctcgtgacg atggcctcca
     4741 tccagaaggc cttcaacaag tggctgcccc gcggcctgga gatgttcgga cacgaaaaag
     4801 gcggcgactc caatgtccgt ttcgggttca agaacatgaa gaacgccgag gcgcagtcgc
     4861 agtactacga cgagtgccgg aagatgatcg cggacttgaa ccagcgctac gttcgcgccc
     4921 ggtctcccca gctctccccc gagcaggccg agaccctggt cgcccgtctc cagaaggggg
     4981 gcggccgcga aaagggcatc gcgagccacg atctcctcga acttcccgat ccacgattct
     5041 ttcgcaggaa gggggaaccc gcatggacga tgatcgggac gcgaggcgaa gcgttcgaca
     5101 ccgtggacgc ctatctcgcc cacttgaagg agcacctgcc ggagccgtac ctggccagcc
     5161 gcgacctgag cgtgtacgtc gcgaccctgc gggacgtggc ggccggaacg gtcgctcccg
     5221 agcaggcgag caagcggatg ccctccttga agcgggtcgg cggagtctgt ccgtgctcca
     5281 agtcggtgcg gtggatcgtg gaagagccac acgccgcgcc cgcggccggc tcgccggcgc
     5341 cgccgcggac agcctcggga gccgcctgaa acaagccgcg gcgcacccgt ttccggggcg
     5401 cagcctgcaa ttcctcgtcg gtctcgcgct gctcggcggg atcatggccg tcctcctgct
     5461 cctctctccc taaaccttcg taccgcgttt tgtggtaggg ttttgggacc cgggaaaaca
     5521 ccgggtcgga gcgtttcacg gacacgaacc ggggggtctt catggttccg ctgaacgttc
     5581 gcgggagggt tttgtccgcc cggcgccgcg tcctgtgcgt cctgacggtc gttctggctc
     5641 tgttcgcggg gtgcgcgccg gcggccaagc aggagagcgt caccgaggtc cggtcggaga
     5701 tcgaggcctc gaacaagcag ttcatggatg cgttcgcgcg ccgcgatgcc gccgcgatcg
     5761 ggctcctgta cgccgaggac ggccaggtgc tcccgccggg cgccccgccg ctcgacgggc
     5821 gcgaggcgat cgtgaccatg tggcagagcg ttctcgcgct tccgttcgcg agcgtcacgc
     5881 tgcagacgag cgacgtcggc gtggacgaag agtccgcctg ggagaccggg cggtacacga
     5941 tgacagggaa cgacggcaag accgcggaaa cgggcaaata catcgtggtc tggaagcacg
     6001 gcgaggctgg atggaagatc tatcgcgata tctggaacag caacaccccc gcgacctcgc
     6061 ccgcgcccgg ggggacgccg gccgctccgt agcgccgcgt ctcgccgaac gccgcggcct
     6121 ggtcagccac gccgcggggg aagccgcctg ggagtgggat ctggagagcg gccggatcca
     6181 gcggggcggc gccatgctcg cgatcaccgg ctacgggccg gaggagatcg gcttcgatcc
     6241 cgcgtggtgg aaggagcgca tccaccccga ggaccgggcc tccgtgctct cggcgatcga
     6301 cgccgccatg gtcggcgagc ggcgcgagct ggtgatgctc taccgtttcc gtcgcaagga
     6361 cggaagctac gcgctgctcc tcgatcgcgc cttcgtgctg ggcgacgacc ccgagcagcc
     6421 ggcgcggatt gtcgggctgg cccgggaagt gaccccgccc gtcattccgc ccgaacgggc
     6481 gccgtcgccg gtcgccgtcg agccggaaga agacccgccc gaggcgggca ctccgtcccg
     6541 acgactgcac gattccgccc tcgcgttcga ccgaatcacc gatcccgccc tcgcctgcct
     6601 tccgctcgac gttctcctga gcgagctcct cgaccgcgtc cgtggcgcgc tgcgcgggga
     6661 tgccgccctg gcactcctct attccgagga cggggccttg ctcgaatgcc gcgcgaccgc
     6721 cgggatccag gccggagcgt cggggcgctt ctccgttccg gcgcggcgcg gcgtcgtcgc
     6781 gcgcgtggcc tcctcggggg cgccgatcct cacgaacgat ccccaggccg cagggggcac
     6841 gccgccggga tgggtacgcc tgtgccgctc cttcgcgggc gctccgatcg ttctggaggc
     6901 gcgcgtcgtg ggggtgctct gcgtcgtggc ccgcgcgcgc ctggagttcg acgaggatga
     6961 ccggaggctt ctggagctcg ccgccgaccg gctcgggccc gccttcgaca gggcccggct
     7021 cctcgagagc gagcgggcgg accgcgaccg gctggcctcg ctctcccgca ccatcgtctc
     7081 gtctctggag gaggagcggc gccgcgtatc gcgcgagctg cacgacgaga tcggccagct
     7141 cctcacctcg ctgcgcctga cgctgcggag cagcgccccg tcacgggtgt cggtggacga
     7201 gatcatggac gagctgttcg cccgggtgcg cgacatctcg acccggctcc gccccccgat
     7261 gctggaggac ctgggcctcg gccccgcgct gagctggcac tgcgagcgct ttgccctgca
     7321 gaccggcgtg cgggtccatc tcatgaccac ggggctggaa cggcgctacc cggccaaggc
     7381 ggagctcgcg gtgtttcgcg tcgtgcagga ggccctgacc aacgtcgccc gccacgccaa
     7441 ggtggtcgac gcgcgcgtcg cggtacgggg cacccccggc accatcagcg tgctggtgat
     7501 ggatcagggg gtcggttttc ccgccggctc ccttcacgaa catcagtcca gcggcctcat
     7561 cggaatgcgc gagcgcgcgc gttccgtggg gggccgcctt ctcgtctcct ccagcccggg
     7621 cggtggaacc cgggtgtcgc tccgcatccc gattcccaaa ggagagacgt ctcccaacga
     7681 cgaggtttcc tcatgaatac ccagccgatc accatcatcc tcgcggacga ccatcgggtc
     7741 gtgcgtgaag ccctccgttc cctgctcgag tcggagcccg acttccaggt ggttgccgaa
     7801 gcctccgacg gactcgcggc cgccgagctg accgagcggc agaagcccga cgtcctggtc
     7861 ctggacctcc agatgcccgg cttgaacggt ctggacgtgc ttcgccgcgt cgtccgccgg
     7921 tccccgcgca cgcgcgtggt cgtcctttcg atgcacgcgg ccgaagggta tgtcgtggcc
     7981 gccttgaagc acggcgcctc cgcgtacgtg accaaggacg cgagcgcctc cgagctgggg
     8041 atcgccatcc ggaaggccat cgcgggccag cgttacgtga gctcgccctt ttcgagcgcc
     8101 gccgtcgagg aatacgcggc gcgatccgag ggggcggccc cggaccccta cgagaccctg
     8161 accggccggg aacgcgaggt gttccacctc gcggccgaag gccgctccag cgcccagatc
     8221 gccgagcgcc tcgggatcag cccgcggacg gcggagacgc accgggcgaa cgccttgaag
     8281 aagctcaagc tgcacggaca gaccaacctg gtcctctacg cggtggagaa ggggctgctc
     8341 ccctccggcg cgcccgagga attgcgcgcg ccgaggccct aggtccggcc cttgcaggct
     8401 cgatgtggcg ggggatccac ggaggtttca ccatgacgga acgcaagcgg acggacaccg
     8461 agacggagaa gaagcgcgtg gaatcggaag aactcgaaaa gacgatccgc gagcttcgcg
     8521 atagcgagga gcggtaccgg cggctggtcg agctttcccc cgacggcgtg ttcgtgcaga
     8581 gcgagggggc gatcgtattc gccaatctgg ccggcgccaa gctgatcggc gccgaccgtc
     8641 cccagaagct cttcggcacg ccgatgctgg agctgttcca ccccgatcat cggagcgaca
     8701 cccgcgcgcg gctggaggaa gcggggccca aggcgccggc ggcgccgttc gaggagaaga
     8761 tggtgcgcct cgacggggcg gtgatcgacg tggaggtggc ggcggccccg ttcacctacc
     8821 aggggaagcc ctcgacccag atcatgatcc gcgacatcac gtcgcggaag gaatcggagg
     8881 agacgctccg gcgccaggcc gagttcatcc gcgccgtcgc cgaatccacg ggcgaggggc
     8941 tctacgccat cgaccgcgcg ggccgcgtca cgttcatgaa tcccgcggcc ggggagatga
     9001 tgggctggac gcgcgaggag ttgttcctgc gcgatctcca ccagatgacc cactaccgcc
     9061 gtcccgacgg gacctccttt ccacgccacg aatgcccgat cctgaccgcg atggagaacc
     9121 gcaccacgat cgagcgcgac gacgtcttca tccggaagga cggctcgatg ctggcggtga
     9181 gcctttcctc gtcgccgctc cgctccgcca acggcgagac ggtcggcgcc gtgatcctgt
     9241 tccgcgacgc gaccgaacgc cggcggctgg aggagcagct cctccacgcc cagaagctcg
     9301 aggcggtcgg acgcctggcc ggtgggatcg cccacgactt caacaatctg ctgagcgtca
     9361 tcgcgggcta cggcgagctg gtgctggccg acctgaagga gccgggcgca gcgcggagcc
     9421 gcgtgctcga gatcctgcgg gccaccgagc gcgcgaccgg cctcacgcgg cagctcctgg
     9481 cgttcgggcg gaagcaggtg atcgagccgc gcgtgctcga cctgaacgcc acggcgcagg
     9541 acgccgagcg gatgctccgg cggctgatcg gcgaggacat cgacatcgcg atgaagctcg
     9601 ctcccgacct cggccgcatc caggccgatc cgggccaggt ggagcaggtg atcatcaatc
     9661 tcgcggtcaa cgcccgcgac gccatgccgc gcggcggcaa gctgacgatc gagaccacga
     9721 atgtcgcgct ggacgacgac gcggtgcgcg gctacgtgga cgtcaagccg gggcactacg
     9781 tgcggctctg cgtctcggac acgggcgtgg gcatggatcg cgagacgcgc gagcacctgt
     9841 tcgagccctt cttcacgacg aaggaagcgg ggcgcggcac ggggctcggc ctggcgacgg
     9901 tctacggcat cgtgaagcag agcggcgggc acgtctgggt ctacagcgag cccgggtggg
     9961 ggaccacctt caaggtctac ttcccgcgct tcggcgacgt ggccgagccc gggcgcccgg
    10021 cgatcgaagc cggtcccgcg cccggcggca cggagaccat cctcgtcgtc gaggacgacg
    10081 agatgatccg ttcgctcatc cgcgacatcc tggaatcgac gggatatcgc gtgctggtgg
    10141 ccgacgaccc ggaatcgggc atgaagctga tcggcgagga gcaggagatc cacctcctgc
    10201 tcaccgacct catccttccg ggcatgagcg gccgcgagct ggtggacaag gtggtcgagc
    10261 tgaagcccga tatccgcgtc ctcttcatgt cgggatacag cgacgaggcg gtggcgcggc
    10321 acggcatcct ggagccgggg ctcgccttct tgcagaaacc cttctcgcgc gacgccttgg
    10381 tgcgcaaggt gcgcgaggtg ctggactcgg cgggatcgcg catcgcgtga ccgacggccg
    10441 tcccagcgcg caccccgcgg gcgccgcgac gccggtcgcc gagacgatgc gggacgacga
    10501 ctgggacgag gtgcgccgcg tctacgccga ggggatcgcg acgaggcagg cgaccttcga
    10561 gacggagact cccgcctggg aggcctggga ccggggtcac cggaaggact gccgcttcgt
    10621 cgcgcgcgcg cccggcccaa gccatcatcg cctcctcggc tgggcggcgc tctccccggt
    10681 gtccggccgc tgcgtctatg ccggcgtggc cgaagtgagc gtctacgttt cggaggacgc
    10741 gcgcggtcgc ggtgtggggc ggatcctcct ggccgccctc atccaggcgt cggagcggca
    10801 agggatctgg acgctccagg ccggcatctt cccggagaac caagcgagcg tgcggctgca
    10861 tcgcgcgctg ggatttcgcg aggtgggaaa gcgggagcgg atcgggcagc tcgatggagc
    10921 ctggcgcgac gtcctgttcc tcgagcggcg gagcgcgatc gccggcgtct gacggcgcta
    10981 cggccggcgc gccagccggt agatctcccc ggagagcagc gtcagataga gttctccctt
    11041 cccatccacg ccgaaggaag agagcccctg catcggcccg ccggccgcgg tacgaagcac
    11101 gggcgtccag tcgcgcggaa cggccggagc tccgctcacc aggcgcaggc tgcgcacgat
    11161 ccccgtgcag tagtcgccat agaagtaggt cccgcgcagc tcgggcagct ccatcccgcg
    11221 gtagacgaag cctccggtca cggagcatcc cacgccatga tcgtactcgg cgatgggcag
    11281 cgtgatcccc gccgggttgc aggacaagcc cggcggatag cagtgcgtcc cttccatgcg
    11341 gttccagccg tagttgcgcc ctccctggcc ggccggctcc acgtcgatct cctcccacga
    11401 gtcctggccc acgtccccga tccacatcgc tccggtctcg gcgtcgaagg agaagcgcca
    11461 cgggttgcgg agtccgtacg accagatctc gccccgcgcg cccgcccggc ccacgaaggg
    11521 attgtcgggg gggaccgtgt agggcgaagc cgcgtccacg tcgaggcgga ggatcttccc
    11581 gagaagggca tccagatttt gcccgttccc ctgcggatcg ccgccgcttc cgccgtcgcc
    11641 cagaccgacg tagagcttgc cgtcgggacc gaaggcgagc tgtccgccgt tgtggttcgc
    11701 gaacggttgg tccacgaaga ggatctcctt ctccgtcgcg ccggcgctgt cgggattggc
    11761 cgagacctgg aattcgacca cgcgcgtgtc cccgctcagg tcggtgtagt ccacgaagaa
    11821 ccggccgttc gcggcgtagc ccgggtggaa ggcgagcccg aggagcccct gctcgctccc
    11881 gccgctcacg cgggcgctca ggtcgaggaa cgggcgggga cggagcgcgc ctccctggag
    11941 gattcggatc gcgccggtct tctccaggat gaagaggcgc gccgtgtcgt cgggcgcggc
    12001 gacggcgccg accggctggg aaaggccgct cgtgacgagg acggcgtggt agtcggcggt
    12061 ggaggccgcc ggcggcggcg ggacttcacg cgaatttccg ccgcacgatg cggtcgcgaa
    12121 gagcagcagc aggagcgcgg cggcccggag cctcatccgt caggttctcc tcaaacatga
    12181 acagccagcg cgataagggt accgcgctgg ctgaggtatt gggagcgacg gggttgctgc
    12241 ggatcgtggt gttcctgccg ccgatcctgc ccgacactcc ccgcccctgc ttccggggca
    12301 acgggctggc gcccttgacg cgcgccagcc tcgttgaata acgataggcg gcccggcgga
    12361 aaacgcaagg ggaggcgggt gatcccgcct cccccggtcc gacgccgatc cgtcccgcgg
    12421 atcagcggtc gtagtcgtcg tcgtcgtcca cgtcgccgcg gtggtcgtcc tcgcagcgat
    12481 cgccacggtc gtagcgcccg tcatcgtagt cgtagtcgcg atcgtatcca cggtcacagt
    12541 agtcaccgcg gtcgcccagg tcccgccagc cgtcgtcacc ccagtcgccg gagcccctcc
    12601 agtcgctgca ctccacccga tatccccgcg gccggtggga gcaggaattc acgtgggtct
    12661 gatacgcccc gtagctgctg aaacgggcat cgcagaagtt gcagccgtag aggtagtcgt
    12721 cgtcgtgata gctgccgtgg aagcgcacgc cgccgatcac gcccgaggcg gcgacgacga
    12781 agcggatcgg acggacgcgg atgacatggc gggaatagat gtactcgggc gggcagtagg
    12841 tgcgccacgc ctggtagcgc ggcccccggc tggcgcggat cacgatcatg tcccgataga
    12901 cgcggcgatc gccccaactc cgccactgcc ggctgaagcc gcgcggcgcg gaatcgcggc
    12961 ggtagatccg ctccacgcgc cgctcgcgac cctgcttcgg ggtccaccac tttccaccgg
    13021 ggtcggcctt cgcgtcgccc ggcgtcgcga gcagcgccgc tcccaccagc gcggccaccg
    13081 ccgctcccat cgtaacggta cgaatcatcg ggtgctcctt tcctgggtcc ttcgagtgac
    13141 gagtctcacc ggcacgtgcg ctgccgccca ttgcaaccca catgccgagg cgccggtcac
    13201 gctcaagtca tgaatagaca atcacatgga cgctctggag tcatggccgg ccagccgcgc
    13261 gccgtgaccg gcgggcgcca tcgcggacca ggtgtcacac acaggccaca gatcgaaaac
    13321 gcggcgctca cctccggagc ggtacctgcc gatacgttaa gccggccccg cggggagcgg
    13381 gccatcgacg agaccaaacc aaagggggac cgaccatggc tgctttctcc gactgggatt
    13441 cgctggagta ccaggccctg atggctcgcg ggcgcgacgc cgagcgcacg gcgctcgtct
    13501 gctggacggc ttcgggcatc gcggcagcga tgctcctctc ctggggcatc gccgcgaaga
    13561 gcgcgagcct catgcttccg gtcatcttcg cgaccgcatt cggcttctac gccgccgtcc
    13621 acgggcgtca gcaggtgcgc ctgatcgcgg gctacgtgaa ggagttcatg gagccgaacg
    13681 gcaattcggg cccgcagtgg ttcacgcggc tcgcgcagct cgaggtcgta cccggcttca
    13741 atccgtcggg cgactgggcc gtggcggcgc tcgcgaacct gacggtggtg acggcgatgg
    13801 ctttctcgtg gatctacgcc ggcggcgcgg gacagcgcgg cgatctcatg gcggggatcg
    13861 cgacgggtct gggactggtc ttctgcttcc attcgatcac ggaaacggcg cgcatccggc
    13921 agaccaacgc cggcgccctc tggcgtcaga tcacgtcgca tgccgaagag cggcggggcc
    13981 cgcgtctcgc ctcgcgcggc tgagacagcc cgcgccgcag cgcggggagc gttcgatcgg
    14041 agcggctccg gcggtttcgc cggggccgtt ttcgttcagc gccgaggggg tgccggagcc
    14101 gtgagggact tggcctcggc cagcgagagg aagcggggcc tgggaagctc gatccggcgg
    14161 ccccggacgt cctggcccgc gaagaaatcc tgcacggcct gctgcacgtc gttgaagggg
    14221 agcacctcgt gccatccgtt ctcgaccacg aggtggattc cgttgggaaa gccccagcgc
    14281 acctcctcgg cctggaaagg cggcgtgatg ccgtccatcg acccggtcag gaacagcgtt
    14341 cgcaccgggc tgtagatccg ttcgcggaac gccggtccca gatccgcgcc tcccaccaga
    14401 tcgcagaacg cgggattcac gagcatgttc ttcacgttgc cgaggagcgc cgtccgcgat
    14461 tcgcgggcga cgacgccgcg ccgctccggc gaggcgcccg acgcgcagtc ggtcgtcacc
    14521 tgcatcaccg agatgcccga cgccgtcgca ttcacgaggt cttcgacgcg ccggacgagc
    14581 agcgtcgagt cgccgttggc gagcgaggcg aacagggcgg ggaggaatgg cgcgcgggtc
    14641 cagtcgttca gatccgagcc caggaccgcc tggatgccgg agggtccgat ccgcatcccg
    14701 acgacctttc ccgtcttccg gtcggtgatc cggagcgtgg ccggccgcca cgacagccgc
    14761 tcgatcagcc ggcgcaccga gccctcgaga tccgggaggt cgtgtcccca ggccgcatcc
    14821 tgcgcgacca gagtcgagac gcgtttcagc tggaagtcgt tcaccgaggg aaggcgccag
    14881 gtcatgtcgg ggccgcgcgt ccccgccagc acggccgcgt cgacccggtc gccgtggcgg
    14941 cggatcatct cgagggcgag ctcggttccg tacgagaccc cgatcaggcg gagccgctcg
    15001 acgcccagcg cgcggcgcag gtcttccagg tcctcggcgc tctcgcccgt gttgtaggcc
    15061 tcgatcgcga tgccgtcgtt gcgcatgaga cgaacgcagg ggcggagcat gctcccgagc
    15121 gccgaggccg tcttggcctc gctctcgaaa gcgtccggcg gaagcgaccc gcggaccgcg
    15181 cactgcaggg tcggatcgga gagacccgat ccgcgctgat cgaggacgat cacgtcggag
    15241 cccgagcgca gcttctcgaa gaactcgaga tagggcggcg tccgcgcgag cagcgttccc
    15301 ggatacccgg gacctcccgg gaggaagacg atcggcgggc cgggggaggt gctcggggat
    15361 cggaagcgga ggaagacgat cgcgatctgc ttgcccgccg gacgggcgtg ctgctccggg
    15421 accgtcagcc ggcccagctc ggcggggcgc tccacgccgt ccagtccctt cacggcgtag
    15481 ggcgcgaaga cgagccgcga gctg
//
LOCUS       DASSLZ010000038         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_900843, whole genome shotgun sequence.
ACCESSION   DASSLZ010000038 DASSLZ010000000
VERSION     DASSLZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900843"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VFM00_01385"
     CDS             <1..973
                     /locus_tag="VFM00_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7496928.1"
                     /translation="FQGGQRIWDIRGARARDAAAEAGIVNQQFVATLNAKQQFFNVLA
                     ARESQIAAAAQLEQAEQQRRTSVALARARQATRSDSLRAEIQVRNAQLAVSDTRNSLE
                     TSEASLTRAVGADELVTAAPGDTLSEGLAMSDSDLAALAERGPLVTQSESLLRAARAA
                     NTMAWTDYLPSASLSYSRGGTGDAADLRVPSDLRYTGALRLSFSVPIFDQLQREERQV
                     QSSVAEDNAQAELRDARLAAREGIVRWLGTYHVAGERVQAQIATVEAAEEDLRVQQQR
                     YAVGGSTLLDVLTSQTQLNQARRDLIQARYDQRVAKAQLEALVGRGL"
     gene            1099..2640
                     /locus_tag="VFM00_01390"
     CDS             1099..2640
                     /locus_tag="VFM00_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7496929.1"
                     /translation="MNRWAATAAGAALLLVVVMLARGAGKKNASVAEIPFEPVSRRDI
                     SLTVEATGTVEPIDLIEVKSKASGQIIRMPVQVGSLVKKGDLLAQIDPRDVQNSYAQT
                     LAALRAAQAKATISLAQKKRSDNLYAQAVITAPEHEAATLDYANAQAAVVKARTDLDL
                     ASQRREDATVSAPITGTVLEQTVASGTVISSATSASGGTTLLKMADLSRIRIRALVAE
                     SDVGNVRPGQSATVTVDAFPNRSFQGTVDKIEPQAVVQQSVTMFPVLISISNEDGLLL
                     PGMNGEVSMLIEQRQDVPAVPVDAVRSAREMPAVAELLGLKPEKVKESLDKQTAAMAA
                     ARRARFAAGGDSARGGGGGGGGGRGADQWSGRGRGGSGGGNGGGFGGGGGAGGTGGVG
                     GGGNGGGGGGGGFGTGAGAGRGRRAQAVFVKTANGIEPRIVRLGLSDFDYAQIVDGLK
                     EGDQVALLSVAEAQAQRQQAKSQIRQRMGSGVPGQPGAGGGGRGAGVGGRGAGGGGGG
                     GGGGR"
     gene            2642..>3802
                     /locus_tag="VFM00_01395"
     CDS             2642..>3802
                     /locus_tag="VFM00_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7496930.1"
                     /translation="MPIFEIVNVALEALRINKLRSFLTMLGIVIGVGAVIAMIALGRG
                     AQESVKQRISSLGTTLLTVFPGQQRGGGGPVASATDRAPLTLDDATALEERGRSILAV
                     QPEMSRQLQIQYENRNTNTNVVGATANYLEVRKFTMQAGRMFTESEDAARRRVAVLGS
                     QVVQDLGAASPELLIGEPIRIGGTQFEVAGVLASKGGGGFQNPDDQVLIPLQTARYRL
                     IGSDRLRSISVLSTSEEDIPVTMAEISKILRREHRLPTGREDDFTIRNQADFLTTLGE
                     TTQVFTALLAGIAAVSLLVGGIGIMNIMLVSVTERTREIGVRKALGATQANILTQFLI
                     EAVVLCLLGGVIGILFGAGTAVVLRKAFGWTTSVGLSSVLVAFIFAGVVGVGF"
BASE COUNT          593 a         1294 c         1393 g          522 t
ORIGIN      
        1 gttccagggc ggccagcgga tctgggacat ccggggcgcg agggcacgcg acgccgccgc
       61 cgaggcgggg atcgtgaacc agcagttcgt cgccacgctg aacgcgaagc agcagttctt
      121 caacgtgctc gccgcgcgcg aatcgcagat cgcggcggcc gcccagctcg agcaagccga
      181 gcagcagcgt cgcacgtcgg tcgcgctggc gcgcgcgcgg caggccacgc gctccgactc
      241 gctccgcgcc gagatccagg ttcgaaacgc gcagctcgcc gtttcagaca cacggaactc
      301 cctcgagacc tcggaggcgt cgctcacgcg cgccgtcggc gcggacgagc tggtgacggc
      361 ggctccgggc gataccctgt ccgaaggact ggccatgagc gacagcgatc tcgcggcgct
      421 cgccgagcgg ggcccgctgg tcacgcagtc cgaatcgctg ctccgcgccg cgcgcgcggc
      481 gaacacgatg gcctggaccg actacctgcc ctcggcaagc ctgagctatt cgcgcggcgg
      541 caccggggac gcggccgacc tgcgggtgcc gagcgacctg cgctacacgg gcgcgcttcg
      601 gctctcgttc tcggtaccga tcttcgacca gctgcagcgc gaggagcggc aagtccagtc
      661 ctccgtggcg gaggacaacg cgcaggcaga gctgcgggac gcgcgcctcg ccgcgcgcga
      721 ggggatcgtc cggtggcttg gcacgtatca cgtcgccggc gagagggtcc aggcacagat
      781 cgccaccgtg gaagccgctg aggaagacct gcgcgtccag cagcagcgct atgccgtcgg
      841 cggctcgacc ctgctcgacg tgctcacgtc ccagacgcag ctcaaccagg cgcgccgcga
      901 tctgatccag gcgcgctacg accagcgggt cgcgaaagcc caactcgaag ccctggtcgg
      961 caggggcctc tagaggagac tatgagccgc tcataccgcg aacccgaagc ccgggagccc
     1021 agccccatcg ttccgggagc gcccgaaacc cagccgcccc caacgtccag gcgcggcggc
     1081 ggcgctggcc ggttccgcat gaaccgttgg gccgcgaccg cggccggggc cgccctcctg
     1141 ctcgtcgtcg tgatgctcgc gcgcggggcc ggcaagaaga acgcgtccgt cgccgagatt
     1201 cccttcgagc cggtctcgcg gcgcgacatc tcgctcacgg tggaggccac cggaacggtg
     1261 gagccgatcg acctcatcga ggtgaagtcg aaggcatcgg gacagatcat ccgcatgccg
     1321 gtgcaggtcg gctccctggt caagaagggc gaccttctcg cgcagatcga cccgcgcgac
     1381 gtccagaaca gctatgccca gacgctcgcc gcgctgcggg ccgcgcaggc caaggcgacg
     1441 atctccctgg cgcagaagaa gcgctccgac aatctgtacg cgcaagcggt gatcaccgcg
     1501 cccgagcacg aggcggccac gctcgactac gcgaacgcgc aggcggccgt ggtgaaggcg
     1561 cgcaccgatc tcgaccttgc gagccagcgc cgcgaggacg ccaccgtcag cgcgccgatc
     1621 accggcaccg ttctcgaaca gacggtcgcg agcggcacgg tcatctcctc ggcgacctcc
     1681 gccagcggcg gcaccaccct cctcaagatg gccgacctga gccgcatccg gatccgcgcc
     1741 ctggtggccg agagcgacgt gggcaacgtg cggcccgggc aatcggcgac ggtgacggtg
     1801 gacgccttcc ccaaccggag cttccagggg acggtggaca agatcgagcc ccaggcggtg
     1861 gtgcagcagt cggtcacgat gttccccgtg ctcatctcga tctcgaatga ggacgggctc
     1921 ctcctgcccg gcatgaacgg cgaggtctcg atgctgatcg agcagcgcca ggacgtgccc
     1981 gcggtcccgg tggacgcggt gcgaagcgct cgcgagatgc cggcggtggc ggagctcctc
     2041 gggctgaagc cggagaaggt gaaggagtca ctcgacaagc agaccgccgc gatggcggcc
     2101 gcgcggcggg cgcgcttcgc ggcgggcggc gactcggcgc gcggcggcgg tggcggcggt
     2161 ggcggtggac gcggtgcgga ccagtggagc ggacgcggac gcggcggctc cggaggcgga
     2221 aacggcggcg gctttggcgg tggcggtggt gcgggtggta ccggtggtgt gggtggcggc
     2281 gggaatggcg gcggcggtgg tggcggcggc ttcggcaccg gcgcgggcgc cggcagggga
     2341 cggcgcgcgc aggcggtatt cgtgaaaacc gcgaacggca tcgagccgcg gatcgtccgg
     2401 ctcgggctct ccgatttcga ctacgcgcag atcgtcgacg ggctcaagga aggcgaccag
     2461 gtcgcgctcc tgagcgtcgc cgaggcgcag gcgcagcgcc agcaggcgaa gagccagatt
     2521 cgccagcgca tgggaagcgg agtccccgga cagcccggcg cgggcggcgg aggccgcgga
     2581 gcgggggtcg gaggccgcgg cgcgggtggc ggcggtggcg gcggaggagg agggcgctga
     2641 gatgccgatc ttcgagatcg tgaacgtggc gctcgaggcg ctccggatca acaagctccg
     2701 ttcgttcctg acgatgctcg ggatcgtgat cggcgtcggc gcggtgatcg cgatgatcgc
     2761 gctggggcgc ggagcgcagg aatcggtgaa gcagcgtatc tcctcgctcg ggacgaccct
     2821 gctcaccgtg ttccccggac agcagcgtgg aggcggcggc cccgtggctt cggccaccga
     2881 cagggcgccg ctcacgctgg acgacgcgac ggcgctggag gagcgtggcc gctcgattct
     2941 cgccgttcag ccggagatgt cgcgtcaact tcagattcag tacgagaacc ggaacaccaa
     3001 caccaatgtc gtgggggcga ccgcgaatta cctcgaggtc cggaaattca cgatgcaagc
     3061 gggacggatg ttcaccgaga gcgaggatgc cgcgcgccgt cgcgtcgccg tgctcggctc
     3121 ccaggtggtg caggatctgg gcgcggcctc tcccgagctc ctgatcggcg agccgatccg
     3181 gatcggcggt acccagttcg aggtcgcggg ggtgctcgcc tccaagggag gaggcggctt
     3241 tcagaatccg gacgaccagg tgctcatccc cctgcagacc gcgcgctatc gcctcatcgg
     3301 gtcggaccgc ctgcgttcca tctccgtcct ctccaccagc gaggaggaca ttccggtcac
     3361 catggcggag atctccaaga tcctccgccg ggagcaccgg ctccccaccg gccgcgagga
     3421 cgacttcacg atccggaacc aggccgactt cctgaccacg ctcggcgaga cgactcaggt
     3481 cttcaccgcg ctcctcgccg gcatcgcggc cgtgagcctc ctggtgggcg gcatcggcat
     3541 catgaacatc atgctggttt ccgtgacgga gcggacgcgc gagatcggcg tgcgcaaggc
     3601 gctgggggcc acccaggcga acatcctgac ccagttcctg atcgaggcgg tggtcctctg
     3661 cctcctcgga ggggtgatcg gcatcctgtt cggggccggc accgccgtcg tcctccgcaa
     3721 ggccttcggc tggaccacgt cggtcggcct ttcctcggtg ctggtcgctt tcatcttcgc
     3781 gggcgtggtc ggcgtggggt tc
//
LOCUS       DASSLZ010000039         3126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1787726, whole genome shotgun sequence.
ACCESSION   DASSLZ010000039 DASSLZ010000000
VERSION     DASSLZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787726"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VFM00_01400"
     CDS             complement(<1..1330)
                     /locus_tag="VFM00_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496931.1"
                     /translation="MTAEPTDIPAPPAPAPRGWGFHALRFFVVGIVLAFLVVSAAVGV
                     LTSLLSGGDPALSRQLVAILNHQVGTDSTRIEARRVSGTLFRGAILERPRLMVKTADG
                     EVTWASAKNVRVDYDLLGILFRKDRSLTAVIDSLTVRVVHDRAGRIVFPKFASHPSGR
                     GTGGTTRVAIAAKNGVFSLDWRNLRFTQVQGRGVLTVGPGQSTLVLDELSGLPDPAAR
                     SRGRVRASGGMTVIGSSLRVDPLVVAYGDSRVRGSVDWDLRAGRAADGLLRLDPLRLG
                     DVSGLVGAGKTDGRLAGEISFEGTPTDGKATACLAGTVNQETLDTLAVTAALAPKRVT
                     FSGLRVRVRGAEATGGGVLALGPGGPLQASITFHGLDPAAVPWWKAPEGMPRGALAGT
                     SRLLVRFSRPRATVVATVALEQSRFGRLDMRQGLLHVATALDGSTVLDSSW"
     gene            complement(1330..>3126)
                     /locus_tag="VFM00_01405"
     CDS             complement(1330..>3126)
                     /locus_tag="VFM00_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET7496932.1"
                     /translation="LRPDYIRFDRLTLQDYYRRHGFIEARVDSVPLRFDAKGNEAQVL
                     FYLTEGPRARVRQVRFEGTGPLPEERLRKVLELKPGDPLDIPKQDLSREAVHNEYAER
                     GYVAAIVRDSLEVDTSGASIVYRIAPGPATHLDSVIVEGTSVTKPSFVRREFTIGRGD
                     LLKRSKLLRSQQRIYDTGFYSDVRFDRSEVDSLGQTADVIVDVRERKMGWVDMGVGYG
                     TVDQLRLTTQLGQRNLWRDGVRLVANGLLGVRVRRSTEGDVHFPYFKLHPKAARLGDS
                     RIDASLTRPWNFGFRVAATVGGYAEHIRPPSSNDQEFPPYRAYGGSAALSYDFTLHTH
                     SRFSYEHRNVVSDTTNLLSTSGAEIQSYTINRIVGSLERDTRDNPFDPRYGTDLLGTS
                     AFVGGALSGSAQFLKHTGTAVAFVPVNEKTTAAFRIRFGAIDPQAAQRAAPGAGANPI
                     SLIPLEERFFLGGANTLRGYGENELGTRTQADSAGNPLPTIRVGGRILLLFNAEIRHR
                     LFGPFGAEAFLDGGNVWERPTDIHARNLLSFGDRAGYNDMRYTAGLGIRFATPLGPLR
                     LDYGWKLRMARPDQRDPATSRGEFHFSVGQAF"
BASE COUNT          480 a         1085 c         1105 g          456 t
ORIGIN      
        1 cccagctgct gtccaggacc gtcgagccgt cgagagccgt cgccacgtga agcagcccct
       61 gccgcatgtc cagccttccg aagcggctct gttcgagggc cacggtcgcc acgaccgtcg
      121 cgcgcgggcg gctgaagcgc acgagaaggc gcgacgtgcc cgcgagcgcg ccgcgaggca
      181 tcccctcggg cgctttccac cacggcaccg cggccggatc gaggccgtga aaggtgatcg
      241 aggcctggag cggtcccccg ggtcccagcg cgaggacgcc ccctccggtc gcctcggcgc
      301 cgcgcacgcg gacgcgaagc cccgagaagg tgacccgctt gggcgcgagc gcggccgtga
      361 cggcgagcgt atcgagggtc tcctggttca ccgttcccgc gaggcaggcc gtggccttgc
      421 cgtcggtcgg cgttccctcg aaggagatct cgcccgcgag ccgcccgtcg gtcttgcctg
      481 cgcccaccag tcccgagacg tcccccaggc gaagcggatc gagacggaga aggccgtcgg
      541 ccgcccgccc ggcgcgcaga tcccaatcga cgctgccccg cacccgcgag tcgccgtagg
      601 ccacgaccag cgggtccacc cggagcgagg atccgatcac ggtcatcccg ccgctcgcgc
      661 gcacgcgccc ccgcgagcgg gccgccgggt cgggcagccc cgagagctcg tccaagacca
      721 gcgtggactg gccggggccc acggtcagca cgccgcgccc ctgcacctgg gtgaagcgga
      781 gattccgcca gtcgagcgag aacacgccgt tcttggcggc gatcgcgacc cgggtggtcc
      841 cgcccgtccc ccgtccggac gggtgcgacg cgaacttcgg aaagacgatg cgtcccgcgc
      901 ggtcgtgcac cacgcgaacg gtcagggagt cgatgaccgc ggtgaggctc cggtccttgc
      961 ggaagaggat gccgagcagg tcgtagtcca cccggacgtt cttggccgac gcccaggtga
     1021 cctcgccgtc ggcggtcttc accatcagcc ggggccgctc gaggatggct ccgcggaaga
     1081 gggtgcccga gacccggcgc gcctcgatcc gcgtggagtc ggtgcccacc tggtgattca
     1141 ggatcgcgac caactggcgc gaaagagccg gatcgccgcc ggagaggagc gaggtgagca
     1201 ccccgaccgc cgccgagacc acgaggaagg cgaggacgat ccccaccacg aagaagcgga
     1261 gcgcgtgaaa gccccagccg cggggagcgg gagcgggcgg cgcgggaatg tcggtgggtt
     1321 ccgcggtcat cagaacgcct ggcctacgct gaagtggaat tcgcctcggg acgtggccgg
     1381 gtcgcgctgg tcgggtcgag ccatccggag cttccacccg tagtccagcc ggaggggtcc
     1441 caggggcgtc gcgaagcgga tccccaggcc ggccgtgtac ctcatatcgt tataccccgc
     1501 ccggtcgccg aagctcagga gattcctggc atgaatatcg gtcggccgct cccagacatt
     1561 acccccatcc aggaacgctt cggccccgaa gggtccgaag agcctgtggc ggatctcggc
     1621 gttgaagagc aggaggattc ggcctcccac gcgaatggtc ggaagcgggt tcccggccga
     1681 gtccgcctgg gttctcgtgc ccagctcgtt ctccccgtat ccgcggagcg tgttcgcccc
     1741 gcccaggaag aatcgctctt ccaggggaat cagggagatc ggattcgcac ccgcgcccgg
     1801 cgccgcccgc tgggccgcct gcgggtcgat cgcgccgaag cggatccgga acgccgccgt
     1861 cgtcttctcg ttcaccggaa cgaaggccac ggccgtgccg gtgtgcttca ggaactgcgc
     1921 gctgccgctc agggcgccgc ccacgaaggc gctggtcccc aggagatccg ttccgtaccg
     1981 gggatcgaag ggattgtcgc gcgtgtcgcg ctccaggctg ccgacgatcc ggttgatcgt
     2041 gtagctctgg atctcggctc cgctcgtgga gaggaggttc gtcgtgtcgg agaccacgtt
     2101 ccggtgctcg tacgagaacc ggctatgggt atggagtgtg aagtcgtagg acagcgcggc
     2161 cgacccgccg taggcgcgat acggagggaa ttcctggtcg ttgctcgagg ggggccggat
     2221 gtgttccgcg tagcctccga ccgtcgccgc gacgcggaac ccgaagttcc acgggcgcgt
     2281 gagcgaggca tcgatccggc tgtcccccag gcgcgccgcc ttggggtgga gcttgaaata
     2341 cgggaagtgg acgtcgccct ccgtggagcg gcgcacccgc acgcccagga gcccgttggc
     2401 gaccagccga acgccgtcgc gccagagatt gcgctggccg agctgcgtgg tcaggcggag
     2461 ctggtccacg gtgccatagc cgacgcccat gtcgacccag cccatcttcc gctcgcgcac
     2521 gtccacgatg acgtcggcgg tctgtcccag gctgtccacc tcgctccggt cgaagcgcac
     2581 gtcggagtag aacccggtgt cgtagatgcg ctgctggctt cgaagcagct tggagcgctt
     2641 gaggaggtct ccccggccga tcgtgaactc gcgccggacg aagctcggct tggtcaccga
     2701 cgtcccctcg acgatcaccg aatcgaggtg ggtggccgga ccgggcgcga tccggtagac
     2761 gatcgaagca ccgctcgtgt ccacttccag ggagtcgcgc acgatcgcgg ccacgtagcc
     2821 ccgctcggcg tactcgttgt gcaccgcttc gcggctcaag tcctgcttcg ggatgtcgag
     2881 cggatcgccc ggcttgagct cgaggacctt ccgcagcctc tcctccggca ggggtccggt
     2941 cccctcgaaa cggacctggc gcacccgggc gcgcggcccc tccgtgagat agaaaaggac
     3001 ctgggcctcg ttccctttcg cgtcgaagcg gagcggcacg ctgtccacgc gcgcctcgat
     3061 gaagccgtgg cgccggtagt agtcctgaag cgtgaggcgg tcgaagcgga tgtagtcggg
     3121 gcggag
//
LOCUS       DASSLZ010000040         4753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_647356, whole genome shotgun sequence.
ACCESSION   DASSLZ010000040 DASSLZ010000000
VERSION     DASSLZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647356"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFM00_01410"
     CDS             complement(<1..652)
                     /locus_tag="VFM00_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7496933.1"
                     /translation="MRWAEPQLLSLLWLVPVLLLALWFAERRRARLERMLGEPRMLRG
                     RAQTPGAGVRWARHALLLLAAALAIAGMGRPQAGFRLVTTTSRGVDLVVALDLSHSME
                     ARDVRPDRLRAAKRELLSLFSALEGSGIGVVEFAGNARVLSPLTTDTQGLESVVETAV
                     PEDLDEPGSNVAGAVRLASLLLRRPGERPRAVLLVSDGENLEGDARAAAGMARGAGA"
     gene            complement(663..1649)
                     /locus_tag="VFM00_01415"
     CDS             complement(663..1649)
                     /locus_tag="VFM00_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7496934.1"
                     /translation="MRFGQPLLLLVLVPAVLVWLLARRRARLRDPELPVPSLALVPAA
                     RGPKAWAAAMAPWLAPLAAALFLVAAARPQAGREVREIVSEGIDILLAIDVSGSMRCE
                     DFRPQNRLFVAKEVAKRFVRGREHDRIGLVAFAGRAELISPVTLDYDGLNALIDGLDF
                     GMLPDGTAVGSAIALGAERLRLTKGKSRVLVLLTDGINNAGSVDPVTAARLAAAVGVR
                     VYTVGAGTQGQAPFPVDDPVLGRHYVWVRSDVDETTLKTIADTTHGRYFRATTAELLD
                     RVYHEIDALEPSRVEMRSYTRWAEAGPKFLGAGLILLALDVLLGATWLRRYP"
     gene            complement(1653..2753)
                     /locus_tag="VFM00_01420"
     CDS             complement(1653..2753)
                     /locus_tag="VFM00_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496935.1"
                     /translation="MIPAPSQLRTRRLAPALLAAAALLVAAGCARRADEHSGATAERE
                     EIRGGRRVVLKSSVSPRRVTLGDPSVWTLTADLPAGAVPGKLLRSAAAPQLDLAAPKA
                     PRVEASRSSGTKGAADRAVYHWPYQIRGFGLGPIALPAVRLPVTYGTARDTLIFPADT
                     LAVDSLTRAASGTVLPDRGPITPELRRVDYVVAAALLLLAIVAALLIARSIRRRRTRG
                     EAPPVPAEPPDVLLRRAIATLRAEGEPMPRDAFYDRLSFAIRDYAAAATGITTRDRTT
                     MEIVRDLRAAGSVPPDGVDALRRALSRADLAKFARRGGGWDEALDVLDTAERLPERLP
                     ARPPAAAESGAAGGTPSGGISSGTRAAGAGKG"
     gene            complement(2750..3628)
                     /locus_tag="VFM00_01425"
     CDS             complement(2750..3628)
                     /locus_tag="VFM00_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7496936.1"
                     /translation="MITPELLAQVRRLTIRSRRAVEEVFSGAYRSAFRGKGLEFADVR
                     EYVVGDDVRAIDWNVTARAGRPYVKRFEEERELTVVVAMDLSGSLAFGSRARTKREVA
                     AEAGALIALAAARNRDRVGLLLFTDRVELYLPPGKSRARTLRVVREMLAYEPKGRGTD
                     LAGALSFLGRVLRRRAIVVLVSDWIASDFDRPLSYLARRHEVVVVEVSDPLERELPPS
                     GLVLARDLETGRVGWADLDSRRGRAEWRAQRARRARGITEAFTRARAGRIALEVGAPV
                     APPLIRHFERRAHRGG"
     gene            complement(3648..4646)
                     /locus_tag="VFM00_01430"
     CDS             complement(3648..4646)
                     /locus_tag="VFM00_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7496937.1"
                     /translation="MPETPSLTLEERTARVREESAFVAPLLAAIEGTIVGQAAMIRRI
                     LIGLFADGHLLLEGVPGLAKTLAVRTVAQAIHARFQRIQFTPDLLPADLTGTLVFDPQ
                     RGAFATKFGPVFTQILLADEVNRAPAKVQSALLEAMQERQVTIGETTYPLDAVFWVLA
                     TQNPIEQEGTYPLPEAQMDRFLLKIKVGYPTPEEESKILDRMGGEVPPLPQPIVHAEQ
                     IVAARHVVNAIYTDERIRRYIVDLVHASREPERFGLDLRPLIQFGASPRATLALARAS
                     RANAFLDGRAYVTPDDVKLLAPDVFRHRILLTYEAEAEGVPVDEVTRRILARVEVP"
BASE COUNT          667 a         1716 c         1803 g          567 t
ORIGIN      
        1 gggcccccgc gccgcgcgcc atgccggcgg cggcacgcgc atccccctcg aggttctcgc
       61 cgtcgctcac gaggaggacc gcgcgcggcc gctcccccgg acgacgcagg agcagcgagg
      121 cgagccgcac cgcgcccgcg acgttgcttc cgggctcgtc caggtcctcg ggcacggcgg
      181 tctcgaccac cgactccagc ccctgcgtgt cggtggtgag ggggctcagc acgcgcgcgt
      241 tccccgcgaa ctccacgacg ccgatgcccg agccctcgag cgccgagaac agcgagagaa
      301 gctcgcgctt ggccgcgcgc aaccggtcgg gacggacgtc gcgcgcctcc atgctgtggg
      361 agaggtcgag cgcgaccacg agatccaccc cgcgcgaggt ggtggtgacg agccggaagc
      421 cggcctgagg gcggcccatc cccgcgatcg cgagagccgc cgcgaggagc aggagcgcgt
      481 gccgggccca gcggaccccg gccccgggcg tctgcgcccg tccgcggagc atgcgcggct
      541 cgcccagcat ccgctccagg cgcgcgcgcc gccgctcggc gaaccacagc gcgagcagga
      601 ggacgggcac cagccagagc agcgacagca gttgaggttc ggcccagcgc acggatggcc
      661 tcctacgggt agcggcggag ccaggtggcg ccgaggagca cgtcgagggc gagcaggatg
      721 agccccgcgc ccaggaactt cggccccgct tcggcccatc gagtgtacga acgcatctcc
      781 acgcgcgagg gttccagggc gtcgatctcg tggtagacgc ggtccaggag ctccgcggtg
      841 gtcgcccgga aatagcgacc gtgcgtggtg tcggcgatcg tcttcagcgt cgtctcgtcc
      901 acgtcggagc gcacccagac gtagtgccgc cccagcaccg ggtcgtccac cgggaagggc
      961 gcctgcccct gcgtcccggc gccgaccgtg tagacgcgaa cgcccaccgc ggccgcgagc
     1021 cgcgccgccg tgaccggatc caccgagccc gcgttgttga tcccgtcggt gagaaggacc
     1081 agcacccggc tcttcccctt cgtcagccga agccgttcgg cgccgagcgc gatcgccgag
     1141 cccaccgccg tcccgtcggg aagcattccg aagtcgagcc cgtcgatcaa ggcgttcagc
     1201 ccgtcgtagt ccagcgtgac cggcgagatc agctccgcgc ggcccgcgaa cgcgacgagg
     1261 ccgatccggt cgtgctcccg cccgcgcacg aaccgcttcg ccacttcctt ggcgacgaac
     1321 aggcggttct gcgggcggaa gtcctcgcag cgcatcgagc ccgacacgtc gatggcgagc
     1381 aggatgtcga tcccctcgct cacgatctcg cgcacctcgc gccccgcctg gggacgcgcc
     1441 gccgcgacca ggaagagcgc cgccgccagc ggcgcgagcc agggtgccat ggccgcggcc
     1501 caggctttcg ggcctcgcgc ggcgggtacg agcgccaggc tgggcaccgg aagctccgga
     1561 tcgcggaggc gggcgcgccg tcgcgcaagg agccacacga gcacggcggg aacgaggacc
     1621 aggaggagga gcggctggcc gaagcgcacg ggtcagccct ttcccgcgcc ggcggcgcgc
     1681 gtgccggacg agatcccgcc ggacggggtc ccgccggcgg cgccgctctc ggccgcggcg
     1741 ggagggcgcg ccggcagacg ctccggaagg cgctccgccg tgtcgagcac gtcgagcgcc
     1801 tcgtcccaac cgccgccgcg ccgcgcgaac ttggcgagat cggcgcggga gagcgcccgc
     1861 cggagcgcgt ccacgccgtc gggcgggacc gacccggccg cgcggagatc gcgcacgatc
     1921 tccatcgtcg tccggtcgcg cgtggtgatc ccggtggccg cggccgcgta gtcgcggatg
     1981 gcgaacgaga gccggtcata gaacgcgtcg cgcggcatcg gctccccttc cgcgcgaagg
     2041 gtggcgatcg ctcggcgcag aaggacgtcg ggcggctccg cggggaccgg aggcgcttcc
     2101 ccgcgcgttc tccgccgccg gatcgagcgc gcgatcagga gggcggcgac gatcgcgagg
     2161 aggaggaggg cggcggcgac cacgtagtcc acgcggcgaa gctcgggcgt gatcggcccg
     2221 cggtcgggca gcacggtccc gctcgcggcg cgcgtgaggg aatccacggc gagggtgtcg
     2281 gccggaaaga tcagcgtgtc gcgcgcggtc ccgtacgtca ccggaaggcg cacggcggga
     2341 agggcgatgg gtcccaggcc gaagccgcgg atctggtagg gccagtggta gaccgcccgg
     2401 tcggccgctc ccttggtccc gctcgagcgc gaggcttcga cccggggggc cttgggcgcg
     2461 gccaggtcca gctgcggcgc cgcggcgctc cgaagtagct tccccgggac cgcgcccgcg
     2521 gggagatcgg ccgtgagggt ccagacggag ggatcgccca gcgtgacgcg gcggggcgag
     2581 acgctgctct tcaggacgac ccggcggccg ccgcggatct cctcacgctc ggcggtggcg
     2641 cccgagtgct cgtcggcgcg gcgcgcgcat cccgcggcga cgaggagtgc cgcggcggcg
     2701 aggagcgccg gcgcaaggcg ccgcgtgcga agctgggacg gcgcggggat cacccgcccc
     2761 ggtgcgcgcg ccgctcgaag tgccggatga ggggcggagc gacgggcgcg cccacttcga
     2821 gcgcgatgcg ccccgcgcgg gcgcgcgtga aggcctcggt gattccgcgc gcgcggcggg
     2881 cccgctgcgc gcgccactcg gcgcggccgc ggcgggagtc gagatcggcc caccccacgc
     2941 gtccggtctc cagatcgcgc gcgaggacca gccccgaagg aggaagctcg cgctcgaggg
     3001 ggtcggagac ctcgacgacg accacctcgt gccgccgcgc caggtaggag agcggccggt
     3061 cgaagtcgga ggcgatccag tcggagacga gaacgacgat ggcccggcgc cggagcacgc
     3121 ggccaaggaa cgagagggct cccgcgagat cggtgccacg gcccttcggc tcgtaagcca
     3181 gcatctcgcg cacgacgcgg agcgtgcgcg cgcggctctt ccccggcggc aggtagagct
     3241 ccacccggtc ggtgaagagg agaagtccca cgcggtcgcg gttgcgcgcc gcggccaggg
     3301 cgatcagcgc gcccgcctcg gcggcgactt cgcgcttggt gcgggcacgg ctcccgaaag
     3361 cgagcgatcc cgagaggtcc atcgccacca ccaccgtcag ctcgcgctcc tcctcgaagc
     3421 gcttcacgta ggggcggccc gccctcgccg tcacgttcca gtcgatcgcg cgcacgtcgt
     3481 cgccgaccac gtactcgcgg acgtcggcga actccagccc ctttccccgg aaagcgctcc
     3541 gataggcgcc ggagaagacc tcttcgaccg cgcgccggct ccggatggtg agccgccgca
     3601 cctgggccag gagctcgggg gtgatcacag cgggcccgcg gccgcgctca cggcacttcc
     3661 acgcgcgcga ggatgcggcg cgtcacctcg tccaccggca ccccttccgc ctccgcctcg
     3721 tacgtgagca ggatgcggtg gcggaagacg tcgggcgcga gcagcttcac gtcgtcgggc
     3781 gtcacgtagg cgcgcccgtc caggaaggcg ttggcgcggg aggcccgggc cagcgccagg
     3841 gtcgcgcgcg gcgaggcgcc gaactggatg agcggccgga gatccaggcc gaagcgctcc
     3901 ggctcgcggc tggcgtgaac gaggtccacg atgtagcggc ggatccgttc gtcggtgtag
     3961 atcgcgttca cgacgtgccg cgccgcgacg atctgctcgg cgtgcacgat cggttgcggg
     4021 aggggcggca cctcgccccc catccggtcg aggatcttcg attcctcctc gggcgtggga
     4081 tagcccacct tgatcttcag gaggaagcgg tccatctgcg cttcggggag cggataggtg
     4141 ccctcctgct cgatcgggtt ctgcgtcgcg agcacccaga agaccgcgtc gagcggatac
     4201 gtcgtctcgc cgatcgtgac ctgccgttcc tgcatcgcct cgaggagcgc gctctgcacc
     4261 ttggccggcg cgcggttcac ctcgtcggcg aggaggatct gcgtgaacac cggaccgaac
     4321 ttcgtggcga acgcgccgcg ctgcggatcg aagaccaggg tcccggtgag gtccgcgggc
     4381 aggagatcgg gcgtgaactg gatccgctgg aagcgggcgt ggatcgcctg cgcgaccgtg
     4441 cgcacggcga gcgtcttggc cagtcccggc accccctcga ggaggaggtg gccgtcggcg
     4501 aagaggccga tcaggatgcg ccggatcatc gccgcctgcc ccacgatcgt cccctcgatg
     4561 gcggcgagca ggggggcgac gaaggcgctc tcctcccgca cccgcgcggt ccgttcctcg
     4621 agggtcagcg acggggtctc gggcatgcgt tggcggatca ggcggcccgg cgggggtaga
     4681 gcgggaaggc cgcgcagagc gcctcgacgt cggcgcggat ctttgcgagc gacgcgggct
     4741 cggcgcgctt ctg
//
LOCUS       DASSLZ010000041         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1661485, whole genome shotgun sequence.
ACCESSION   DASSLZ010000041 DASSLZ010000000
VERSION     DASSLZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661485"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1968)
                     /locus_tag="VFM00_01435"
     CDS             complement(<1..1968)
                     /locus_tag="VFM00_01435"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7496938.1"
                     /translation="MESIQPGQLLGHYRIVSRLGTGGMGEVFRAEDLRLERPVAIKLL
                     PPALARDAEAVERLTREARSASSLHHPGIVAIFAIEETEGAPYIVMELVEGETLHERI
                     KRGALDASELVSIGTQVAEAIGAAHQVGLIHRDIKPSNVLLTRGGKAKLADFGIAKRL
                     VAPNADPMATAALSLTATGALVGTFMYMSPEQSRGEALDTRSDLFSLGVVLYEAATGR
                     LPFEGPTALSVLHEIALVEPPAPSRIRAGLPRGLDHVLLRAMSKDREGRYATAEEMAA
                     ALREALSESEEEAKVTRTAPFTIEPGSAAIPNNLPSALTSFVGRRDEMEEVGRQLRSG
                     RLVTLAGAGGCGKSRLSIQIARNLLQSFPDGALLVELAPLSDASLVVQVVAGVFGIRE
                     MPGRPLLQTLKQSLSGRTFLLILDNCEHLIPGCVEIARTIMEACPHGRVLATSRTPLG
                     IPGEVVWRVPPLRIPAVSTGASRSRKEVGAYESVRLFVERASTVMPQFALTDENAEAI
                     AQICSRLDGIPLAIELAAARIKVLPVGQILARLEDRFRLLTAGSLAALPHQQTLRAAV
                     DWSYELLTDPERSLFQRLSVFAGGATLEAVESVCSGEGLAEDLVLDLMTQLVDRSLVI
                     PEEGAGGTARYRLLETLREYGKERLVAAGADA"
     gene            complement(2030..2102)
                     /locus_tag="VFM00_01440"
     tRNA            complement(2030..2102)
                     /locus_tag="VFM00_01440"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2067..2069),aa:Lys,seq:ctt)
     gene            2259..>3211
                     /locus_tag="VFM00_01445"
     CDS             2259..>3211
                     /locus_tag="VFM00_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET7496939.1"
                     /translation="MNQSLRFSAGLALALAIGLVLAGGVWTCARASEKPPKVLVIGVD
                     AGEWDVLGPLLDQGKVPTFARLRDKGASGRIRSLEPLTKSPIIWASIATGKVPSKHGI
                     SDFFVKRGERARARTGAEGESPTTSNLWKARPVWDILGSLGKKVGVVGWWTTWPAQPV
                     NGFVISDYVQYDDGSWPSRDSRRTYPADLDALTKQLRRTPESVSWAELFQFVPAFDTT
                     KVTPRQEQLVHDLKWIYAADMTFYRVGLELYRKNHPDFMTVYLRGTDEISHRYWDMDM
                     PGTFSPPLSDAEYQWMKNLIPNYYVFTDRLIGNLLKEADPST"
BASE COUNT          555 a         1095 c         1067 g          494 t
ORIGIN      
        1 ggcgtccgct cccgcggcga ccaggcgctc ctttccgtat tcccgcagcg tctcgaggag
       61 acggtagcgc gccgtgcccc cggctccctc ttcggggatc acgagcgagc ggtcgaccag
      121 ctgcgtcatg aggtcgagga cgagatcttc cgccagtcct tctccggagc agacggattc
      181 caccgcctcg agcgtcgcgc cgcccgcgaa gacgctcagc ctctggaaga gggaccgctc
      241 cgggtcggtc aaaagctcgt agctccagtc cacggcggcg cggagcgtct gctgatgcgg
      301 aagcgcggcc aggcttcccg cggtcagcag gcggaaacga tcttcgagcc gcgccaggat
      361 ctgcccgacc ggaaggacct tgatccgggc cgccgcgagc tcgatggcca gaggaattcc
      421 gtcgagccgc gagcagatct gcgcgatcgc ttcggcgttc tcgtcggtaa gggcgaactg
      481 gggcatgacg gtcgaggcac gctcgacaaa aagccgcacc gactcgtacg cgccgacctc
      541 ctttctgctt cgcgaagcgc ccgtcgagac ggccgggatt cgaagaggcg gcacccgcca
      601 tacgacctcg cccgggatac ccaggggcgt gcggctggtc gcgagcaccc gaccatgagg
      661 gcacgcctcc atgatggtcc gcgcgatctc gacacacccc gggatcaagt gttcgcagtt
      721 gtcgagaatg aggagaaacg tccgccccga gagggactgc ttcaatgtct gaagaagggg
      781 acggccgggc atctcccgga tcccaaagac gcccgcaacg acttgtacga cgagggacgc
      841 atcggagagg ggagccagct cgacgagcaa ggcgccgtcg ggaaaactct ggaggaggtt
      901 ccgcgcgatc tggatcgaga gccgtgactt gccgcaccct cccgctcccg cgagcgtgac
      961 gagacgcccc gaccgaagct ggcggccaac ttcctccatt tcgtcccgtc tcccgacgaa
     1021 actcgtgagg gcggacggca ggttgttggg gatcgccgcg gatcccggct cgatcgtgaa
     1081 cggtgcggtg cgcgtgacct ttgcttcttc ctcggactcg gacagggcct cgcgaagcgc
     1141 cgccgccatc tcttccgccg tcgcgtagcg cccctcccga tccttggaca tggcccgaag
     1201 gagcacgtgg tcgagccctc tcggaagccc cgcgcggatc cgcgaaggcg cgggaggctc
     1261 gacgagcgcg atctcgtgca ggaccgagag ggcggtcggg ccctcgaagg gaagacggcc
     1321 ggtcgcggcc tcgtacagca cgactccgag ggaaaagagg tccgagcgcg tgtccagggc
     1381 ttcgccccgc gactgctcgg gagacatgta catgaaggtc cccacgagcg ctcccgtggc
     1441 ggtgagggag agcgccgccg tcgccatcgg atccgcgttc ggggcgacca ggcgcttggc
     1501 gatgccgaaa tcggccagct tcgccttgcc tcccctcgtc aggagaacat tggagggttt
     1561 gatgtcgcgg tggatcaagc ccacctggtg cgccgccccg atcgcctccg cgacctgcgt
     1621 cccgatcgag accagctcgg aggcgtcgag agcgccccgc ttgatccgtt cgtgaagcgt
     1681 ctccccttcc acgagctcca tcacgatgta gggagcgccc tcggtctctt cgatggcgaa
     1741 gatcgcgacg atgccggggt gatggaggga ggatgcggac cgggcctcgc gcgtgagccg
     1801 ctcgacggct tccgcgtccc gggccagcgc gggcggaagg agcttgatcg cgaccggcct
     1861 ctcgagccgg agatcctcag cgcggaagac ttctcccatc ccgccggtcc caaggcgact
     1921 gacgatgcgg tagtgaccca gaagctgccc gggctggatc gattccaccg aagctcctac
     1981 gcggtgtggg gtgtccggaa cgtcacggag cgataggcac ggaagggttt gcgccgccta
     2041 ggaatcgaac ctagaaccta ctgattaaga gtcagttgct ctgccaattg agctagcggc
     2101 gcgcacgtaa gacaggcgga cgcaccaagc tacgggacag ggcccccaaa gtcaatggga
     2161 accgcgaccc gcccgtccgg gtgtcactgg aacgcggcca ccatgcctga taccgtcatc
     2221 ggattggccc ggcgttcgcc catctccaag gagcttccgt gaaccagtcc ctccgcttct
     2281 ccgccggcct cgcgctggcg ctcgcgatcg gcctcgtcct ggcgggaggc gtctggacgt
     2341 gcgcccgcgc gagcgagaag ccgcccaagg tccttgtcat cggcgtcgac gccggcgaat
     2401 gggacgtctt ggggccgctc ctcgaccagg ggaaggtccc gacgttcgcg cggctccgcg
     2461 acaagggggc ctcgggacgg atccgctcgc tggagccgct gaccaagagc ccgatcatct
     2521 gggcgagcat cgccaccggc aaggtgccga gcaagcacgg catctccgac ttcttcgtga
     2581 agcggggcga gcgggcgcgg gcgcgcacgg gcgccgaggg ggaatccccc accacctcca
     2641 acctctggaa ggcgcggccg gtctgggaca tcctgggctc gctgggcaag aaggtgggcg
     2701 tcgtgggctg gtggacgacg tggcccgcgc agccggtgaa cggcttcgtg atcagcgact
     2761 acgtgcagta cgacgacggc tcgtggccct cgcgcgattc gcgccggacc taccccgccg
     2821 acctggacgc cctcaccaag cagctgcgcc gcacgcccga gagcgtctcc tgggccgagc
     2881 tcttccagtt cgtccccgcg ttcgacacca ccaaggtcac gccgcgccag gagcagctgg
     2941 tccacgacct caagtggatc tacgccgccg acatgacgtt ctatcgcgtg gggctggagc
     3001 tgtaccgcaa gaaccatccc gacttcatga ccgtctatct gcgcggcacc gacgagatct
     3061 cgcatcgcta ctgggacatg gacatgccgg gcaccttctc gcctccactc agcgacgccg
     3121 agtaccagtg gatgaagaac ctgatcccca actactacgt gttcaccgac cgcctcattg
     3181 ggaatctcct gaaagaggcc gatccctcga c
//
LOCUS       DASSLZ010000042        20007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_15659, whole genome shotgun sequence.
ACCESSION   DASSLZ010000042 DASSLZ010000000
VERSION     DASSLZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 20007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20007
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15659"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFM00_01450"
     CDS             <1..387
                     /locus_tag="VFM00_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496940.1"
                     /translation="VFREKLTDFVSDGLERAVGVGGTVTAFGALDLNLTKYDPTRIEN
                     HLLSRERIAAIEKQLCSIPLANRRDLAGVSRGRADIIPAGAVILSEFVNRFPVAGITV
                     STRGLRYGLVLSEARKHRKRNVQSTS"
     gene            765..899
                     /locus_tag="VFM00_01455"
     CDS             765..899
                     /locus_tag="VFM00_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496941.1"
                     /translation="MAPSFTAIRGTSSSVRTTRVTTTLSIWKAYLTALRLGYGVRWLW
                     "
     gene            987..4115
                     /locus_tag="VFM00_01460"
     CDS             987..4115
                     /locus_tag="VFM00_01460"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7496942.1"
                     /translation="MDGLVPRDRPNIKSSGDLEETDARASVSEGDEHRAQELGDLYFA
                     ADNYAVALEYYRRALDAEASKNGTADRDALFRLGARIVDCLRYRGDLDEAVDALHDLH
                     HRLRPHVTPEQIGRLAGSLGILLFDRGRYRAAQRSSVLAYRLLRETTLNFDIGRAEMT
                     LGWIALRTGSWSSSRDYFESAIATYRRADFQAGMASAYNSLALVHKNQCRFKEAARFL
                     EQALRISERAGLYQDSGTYLHNLGIVHEKMGEWDLAEEHYRRALQIYTETGFAAGKAR
                     SLNHLGILRRKRRDFSGAESLIREGLALATERNCRREIVLAHEALGDWLADLGRFPEA
                     RTEYDTGLALADQLATDSDLQVELWRRIGDAQVRMGELDAARRSGERALAIARRLGDR
                     IEEACSLRLLGLRAAEAGEWEEARGFIDEAARILSGISKRYELAKLNHAVGIVWRKRG
                     RKGRSRGVLDESIGYLRRAAAGFETLRLPVHTARALLELARSELLRGLMDDAVIHVDH
                     ASSLLTPEEDPELARDIELFRSEVEQGLIDGSSSQTNEFAAFDEIRTALRGGDAETAL
                     QEILSIVARRAGATRGIVAAPAAQGGIEAVATVGMANRLAHDLIRELERRIGPARLAS
                     GPIVTSRAGTDERFRDLPATFHIGTRTTVAVVPLSLPSGLPGYLYLDRSEEPLGAFKQ
                     REINLITVLANHVAVAILERQRQRLARENSALRGRLLGSANDHGIVTRSPDLLEILSL
                     LERVGPTDATILIEGETGSGKGLLARAIHASSVRTNKPLVQVNCAALPEQLLESELFG
                     HVQGAFTGAVRDKAGLFVEANGGTLFLDEVDKTTITIQGKLLHVLDNREVRPVGGNRP
                     TKVDVRVICATNVNLRARIARGEFLEDLYYRLNDICFRVPPLRERPEDIPILLEHYIR
                     RFSVEMSKEVGGVDPELIRTFSSLPWRGNVRELEKVVKRMVVMANEGEVLSLRLVPRD
                     ILRKEDDVAEGPATLRSEVAKVERRMIAQALERHNWNKLQAARELSLSYPTLLQKIKL
                     FHLDRRGTRQRA"
     gene            4832..5611
                     /locus_tag="VFM00_01465"
     CDS             4832..5611
                     /locus_tag="VFM00_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496943.1"
                     /translation="MTRTLLLALGLAAVALTAGCRNEDHVVGTSNPPFDVEGVYSVTG
                     DGQVSIYWRENQENDIDYYKVYRNSAPTGTFTSIGQADHPPFIDTHVTNGTTYYYAVT
                     AVDTRGQESPDLSFENVFDTPRPEGTNVTLTSAATTAATAGWDFSAMSRVASTSASAD
                     IWFDASSGALVYAGTGVKIQDAGLIDLVDVDFGPPASDGWSADGIVEAIPGHSYIVLT
                     ADNHYAKFEVVSKAAGSIVIDWAYQIDPNNPELAPKIARKN"
     gene            5611..7089
                     /locus_tag="VFM00_01470"
     CDS             5611..7089
                     /locus_tag="VFM00_01470"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HET7496944.1"
                     /translation="MKRARWRRLFVAGAAVLMAAALPVASRAATRVDASISTPRVDAS
                     ISYEAAHSPRLDIDVWTNKEEGGVYRQGEPMRVLFRPSADAYVLIYNIDTEGYIHLVY
                     PYDPGDPDMVEGGRTYQVPSRNDPYDLVADGPTGIEYVVAIASPEPFRDLPWYLTGGY
                     EGRDRGQDYGDDDEGDSGVIAGDPYIGMDRVVRRVVPRGLEDEVATADTYFYIERKVE
                     YPRYVCADCHSYGGWFDPYGDVCSVVDIRIDAGWAHYRPLRSRILRPRYFYMVRSTAP
                     TRYRQWKDRWSSVDGRTTLRTRFQVDGTDKFQSRRQTIQRRTPPEFQRFRSPREGRVW
                     RPDEVLRRGGGDNRGGDVRDRGDVRGGGDRGDAGDTRRREDIRQNQDQRRREWIEMRE
                     RRQNDQRQNDQRQNDQRQQDQRQMRERRDDQRREDQQSRERARAEDRRQERQDRDQAR
                     QRDDRSQDRGDRGRSEERRRDDGGRRQDRRDQDGGGWGRRGR"
     gene            7282..10404
                     /locus_tag="VFM00_01475"
     CDS             7282..10404
                     /locus_tag="VFM00_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HET7496945.1"
                     /translation="MPWKHGPAPREGFSRDRLILPPPGARGLAARAPLGAQATINILA
                     IRVAFSDTPIESTTAYYDRLLLFQKQFWEQETEGSRTLTATLVDSVFTLPHPMAYYGD
                     DDRFQERLVFMVRDLVQLADSTVDFAPYQSLLIFHAGKGQEADVRDDSKDEIWSAFVT
                     QQDFQTVLPDTTGAGRVGIRTNDLSGSNPVYIKEAVEVPESESQDGFVFGMTGVVCHE
                     FGHQLGLPDLYDTNGDVGGLSQGVGSWDVMGTGVWNFSGFAPAGLSAWSKIFLGVIAA
                     QRVVTPQPISLSMLERQVGSLPRAVQIPMTETEYLLLENRRQDLNDNGRFDFDDADAD
                     SAFDFYIDSYAGAEFDFYTPGDGAGSGILVYHVDDSVIAAGLLDNVVNSNAARKGIDI
                     VEADGIEDMDGPPSSYSDGSPDDVFRAGWRDKLTPATTPSTAAYGNVPTGISITGISA
                     PDSVMTFDLSFLQDKPGWPKLLNGRVRSAPSLAADVDGAGPPFTQELLVPVQRLNNTG
                     ALYIFRPDGNDFLDGDATPTPFASTTSGVVTSPCVGDVDGAPGNEIVFSTANGTLYAF
                     HANGTEVVDGDNNPLTLGVVTAGFATGVRAQPVLAEVDGTPGLEIVIGSPATATGYSF
                     VRVIRLAGGVLEEHSMLVGGSSEAPAVATGLEGGANQVVIAARRTVIGENSLPGLYLL
                     NWEILTDPVRDPLDPENIPGTRLAAGQFSAPVAADLEGNGKHEIVVADTTGLFHAYRV
                     AIGAHIPGDDPAGYVSTTELPGWPTPFPGVSRGRASEVSVADLERDGHPEVFQTGENA
                     RVAAFYWTGAPRAGYPLRLAAPGAPADSAGSWAPIVADVDGDGLRDVIPILPDGRRLA
                     FRGDGSPIGGFAELGSTGLSAPPILADLDGDGPAEWIETFDASSQAQIDVKIPVLPIA
                     AASIGWGQYRLSPTRNAVVDAVTGGPAPGTQGLSEVYVYPNPSRDGTSRVHYRLEAAA
                     TSVSIRIYDTSGSLVADLSTGAADRLGSSEHSVAWNNGSIASGVYVCRVEVQSGGRTE
                     VRFANLAIVR"
     gene            10425..11345
                     /locus_tag="VFM00_01480"
     CDS             10425..11345
                     /locus_tag="VFM00_01480"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET7496946.1"
                     /translation="MRPTCRGIAAAFASWIVLALAPHAALAAAGETGFASLKLGVGAR
                     PMGMGSAYVALVDDPTATYWNPAGLAAVRSTQITAMHNEWIQDFRQEYAAVAAPLGKG
                     TLGFAFSGFYTSELEGRDEVGNVTHNFGFNDITMTAGYGRSLAPGLDAGLAVRYLREM
                     IDDQDGATFAADLGARYRVKETGLSFGAAAQNFGGKPKLVDQSFKLPATLRLGAAWSK
                     PIAALHSTGTLTTEIRKARAEDARFHVGGELSYKDRLALRVGGKFGYDAEDVSFGIGL
                     TQSKLRFDYALVPLSSDLGTAHFFSLTARL"
     gene            complement(11430..12164)
                     /locus_tag="VFM00_01485"
     CDS             complement(11430..12164)
                     /locus_tag="VFM00_01485"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HET7496947.1"
                     /translation="MPDRGCAYFVADAHLGQGPLDSSRARERDLLAFFDRVASEAAEL
                     YVVGDLFDFWFEYGHAIPKRFVGVLTALGSLARSGVPVTYLGGNHDFWIGDYLRNELG
                     VAFTDQPMALALQGRKIFLAHGDGLGPGDHGYKLLKRVLRNRAAQFLFRWIHPDIGIP
                     IASATSHTSRHHAPRPKRTEAEMQALLAEPRFAEGFDAVLLAHFHRPIHQRANGRDFI
                     VLGDWIERRTVVKLERGVFSMFEFGG"
     gene            12326..12790
                     /locus_tag="VFM00_01490"
     CDS             12326..12790
                     /locus_tag="VFM00_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496948.1"
                     /translation="MAPQTGFWDRLQDEVRTGYERTRRTTTRTVRIGVLRVDLLSLRR
                     DRTRALSQLGERAFQLWNGGSLAVLDGDEEALRLKSLVEGIERCIADKEAELQSLRKE
                     ADEADAGRPAENASGGAAPGTTPDDPPAERQTGFTESGGPRPPRMEGMNGIT"
     gene            12777..14285
                     /locus_tag="VFM00_01495"
     CDS             12777..14285
                     /locus_tag="VFM00_01495"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HET7496949.1"
                     /translation="MESRETVRAAKVLLVAGGLLLIGIVVGFILAGGSNSSPVLQAKE
                     SVVSTGLESPFTAIADRTLPGVVSIETKRNVTGSDGPSAQFEGPYGDFFRRLFPETPQ
                     QPQQQRRNIRIPSSGSGFIIDHAGYILTNNHVVRDAAEITVTLNDHRKFKARVVGTDP
                     GTDVAVIKIVGAGDLPVVPLGDSDEIRIGDWAIAIGNALGELDGTLTVGVISAKGRTN
                     LAIQGGAPSYQNFIQTDASINFGNSGGPLLNIRGEAIGINTAVNPSGEGIGFAIPINI
                     ASSISKQLIATGKVTRGYLGILPQELTPELADNWGIQGHGVLVGGVSTNTPASQAGFQ
                     VKDVITDFDGKPVDDVQRFRLLVAETPVNKKVAVRVLRNGKPRDLAVTLAERPPEDQV
                     AKADNAIPNSTSDWLGVQVSALGSDAAGEFNLHDKTGVVITDVTEGSGADDSGLRPGD
                     VIKEVNDIPVKDMVEYTHAVEAAKRKNPAKPVVFLIKRGDATQFVAVEPSQD"
     gene            14305..15570
                     /locus_tag="VFM00_01500"
     CDS             14305..15570
                     /locus_tag="VFM00_01500"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7496950.1"
                     /translation="MRERSTRPAARSLRGARAGLGRVAPLAFLLLLAVGCFLGAAPAA
                     TSAAPAPAAPELVRLRFWTAPEHTRLVFDLSGAAPAATKFRMIDSTTFEVFMPRVRKS
                     PAVTTEFVGDSLVADIFPAVSDSGTSVRIHLKKKVTPLGFVLAAADGVPDRVVVDVPM
                     PVNPAAEQALQQKVAELKSGKKMFIVAIDAGHGGEDTGAIGYRHLQEADITLAVARKL
                     KSQLDLIPGISAVLTRNSDYFIPLRKRIDLARKYQADLFVSIHCNASRNHDATGTEIY
                     FLSLNGATDEASRSLAEKENAADMIGGVPPESGDDLLSILFDLRQNDTVRRSSELAEL
                     LIDAVSADTRLSTRGVKQAGFVVLKAPEIPSVLVETAFITNPREAAILKDTQFQNKFA
                     EHLAQGIQSYKAMRDRASGGAAAPTGSSN"
     gene            15666..16178
                     /locus_tag="VFM00_01505"
     CDS             15666..16178
                     /locus_tag="VFM00_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HET7496951.1"
                     /translation="MNRILSKYAPLAAAALLVAGSAFAGGASCGSGDAPRSASAGHHC
                     EGMKAEGASAMSCPTVKAGQNIYSFAVPGAECENCVKSIQTALMAQKGVACAHVDLTS
                     HTAYVIADRSFDRNAIAKCIQTAGFKNKYKGQGAKVQAEFAKAMSAPSGKASACPYKE
                     SKESKEKDKI"
     gene            16130..17386
                     /locus_tag="VFM00_01510"
     CDS             16130..17386
                     /locus_tag="VFM00_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496952.1"
                     /translation="MSVQGEQGKQGEGQDLNRPGAVFALSLFFGACLSSGTPRFAAAS
                     AESPIDWEAHLDLVQVSRTDQINSNFFSFAENPFHAIRLRLLADGRIQEHISAFAEIL
                     SDDGRAPRLYGGFVRFSDPKGRDIHLEAGKIPMHVGAFPNRSDASRNNLIGSPLVYQY
                     HLDVRDDQVPVRPEDIVANQGRGYFTNYAAGGLTGLGYPGTGQALSVLYENCWDVGAV
                     VVGTARPFEFALGVTNGTVGSMVSTDDNDGKQVLGRVGLAPAPWVRAGVSGARGPYLS
                     RALEESALPARKSADDYNQVFAGGDLELSYDRAVVYGEYVWNRFEQPWVGDLDLRAWY
                     AEGKVTVLPGWYVAGRYSRVAFDNLTLAGGRAAAWDAPIWRREIGIGFKPSKMLLAKL
                     VHQETHTETVPRRVEAFMAAQLAVVF"
     gene            17414..17803
                     /locus_tag="VFM00_01515"
     CDS             17414..17803
                     /locus_tag="VFM00_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7496953.1"
                     /translation="MRFLGKYAETLFAIMRIVVGFLYLCHGGQKLFGWFGAEKAAEPL
                     MIAAGVIEFGGGLLVAIGLVASAAAFIASGEMAAAYFMVHAPHSFWPIVNHGELPALY
                     CFVFLYIAAHGSGRLSVDAARRGATRT"
     gene            17925..19865
                     /locus_tag="VFM00_01520"
     CDS             17925..19865
                     /locus_tag="VFM00_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7496954.1"
                     /translation="MITPEPSRAGAIPERSFDSESPLDDTVFRQIVQGARDYAIFLLS
                     PEGYIRTWNLGAERIKGYTPSEIIGQHFSRFYTQDAIDRGWPMRELEIAKAEGRFEDE
                     GWRLRKDGSRFWANVIITAIHDESGELAGFSKITRDLSERRRHEESLRESEERFRLLL
                     EGVSDYAIVFLNPEGYVSSWNVGARRIQGYGADEIIGRHFSLFFPSEAKSAGAPQLLL
                     DQARATGRAEDDGWRTRRDGSRFWANEVITALYDHEGSLRGYAKVTQDMTQRRKIEVL
                     EETARRVNEFLAMLAHELRNPLAPIRNAVGLMRAKRIDDPTLQWSRDVIERQTAHLSR
                     LVDDLLDVSRITSGRLTLQRERLDMAVVFERAVEASRPLIEGRRQALEVRLPETPLWV
                     EGDLIRLAQIVLNLLNNAAKYTPAGGRIWLSGELEAGKAVIRVRDSGVGISSELKTRI
                     FDLFTQGARTLDRSDGGLGIGLTLVERLAALHGGTVEVVSAGANQGSEFIVRLPAAAP
                     REAAPGKDAAGAGSGHAARRRVLVVDDNRDATETMSMLLGVWGHDTRSANDGPGALTV
                     AAAFQPEVVLLDIGLPGMDGYEVARQMHALPGLRNAVLIAMTGYGQEEDRMRSRSAGF
                     ARHLVKPADPSALRQLLESLPE"
BASE COUNT         3279 a         6944 c         6744 g         3040 t
ORIGIN      
        1 gttttccgtg agaagctcac cgatttcgtc tcggacggcc tggagcgggc ggtcggcgtg
       61 ggaggcacgg tcaccgcctt cggagccctg gacctcaatc tgaccaaata cgacccgacc
      121 cggatcgaga accacctcct ctcgcgggag cggatcgccg ccatcgagaa gcagctctgt
      181 tcgataccct tggccaaccg ccgggacctg gccggcgtca gccggggccg cgccgacatc
      241 atcccggcgg gggcggtcat cctctcggag ttcgtcaatc gtttccccgt ggcggggatc
      301 accgtgtcca cccggggact ccgatacggg ctggtcctca gcgaggcccg gaagcaccgg
      361 aaacggaacg tccagtccac gtcgtagggc tggaatcagc cattttcgcc ttgacgattc
      421 caacttcctc cgatatcatc agttattaag cggcagtgtt cgttgacagg tgtccgtccc
      481 atcctgggac gtccctcaaa gctctcggtc acgcttccgg gagcccggca cctttcggcg
      541 aactgccgtt gaaagtgggg cggctgttac gccggaagcc gttcacactg ggagcaggca
      601 tgaggatccg ggttccacga cgcttaatgc tactgggtgt cctcctgtgc tcgctcgcgt
      661 tcgtcactgc cgttccgcag acttctctgg ccaaaccaat tccatgggat cctgccaacg
      721 gtcccgcccc cggcgacggg gacggctcgg ttgtgaaggg caccatggcg ccgtcgttca
      781 cggcgatccg gggaacgtcc agctcggttc ggacgacaag agtgacgacc acgttgagca
      841 tctggaaggc ctacctgacg gcactgcgtc tgggatacgg ggttcgctgg ctctggtaga
      901 acccgcgcgc ccgcgctgac gtagcgtgcg cgcatcggtc tacggatcag ccccccggtt
      961 ccccacgtgg tgggtagagg gccggaatgg acggactagt ccccagggac cgaccgaata
     1021 tcaagagcag tggtgacctc gaggagacgg acgcgcgagc gtccgtctcc gaaggggacg
     1081 aacaccgcgc tcaagagctc ggcgatctgt acttcgccgc ggacaactat gccgtggcgc
     1141 tcgagtacta ccgtcgcgcg ctcgacgccg aggccagcaa gaacggcacg gcggaccgtg
     1201 acgcgctctt ccgtctcggc gcgcgcatcg tcgactgcct ccgttaccgg ggcgatctgg
     1261 acgaggcggt ggacgcgctc cacgacctcc atcaccgtct ccggccgcac gtcacccccg
     1321 agcagatcgg ccgcctggcc ggcagcctcg ggattctcct cttcgatcgc gggcgctatc
     1381 gcgccgcgca gcgctcctcg gtcctcgcct atcggctcct ccgcgaaacc acgctgaact
     1441 tcgatatcgg ccgcgcggag atgacgctgg gttggatcgc gcttcgcacc ggcagctggt
     1501 cgtcgtcgcg ggattacttc gagagcgcga tcgcgaccta tcgccgcgcc gacttccagg
     1561 ccggcatggc ttcggcgtac aacagcctgg cgctggtgca caagaatcag tgccgcttca
     1621 aggaagcggc gcgcttcctg gagcaggcgc tgcgcatttc cgagcgcgcg ggcctctacc
     1681 aggactcggg gacctacctc cataacctgg ggatcgtcca cgagaagatg ggcgagtggg
     1741 atctggccga agagcactac cgccgcgccc tccagatcta caccgagacc ggcttcgcgg
     1801 cgggcaaagc ccgctcgctg aaccatctcg gcatcctgcg gcggaagcgc cgcgacttca
     1861 gcggcgccga gagcctgatc cgcgaagggc tcgcgctggc gacggagcgg aactgccgtc
     1921 gcgagatcgt gctggcccac gaagcgctgg gcgactggct cgccgacctg ggccgtttcc
     1981 ccgaggcgcg caccgagtac gacaccggtc ttgcgcttgc cgatcagctc gccaccgact
     2041 ccgatctgca ggtggagctg tggcgccgga tcggtgacgc gcaggtccgc atgggcgagc
     2101 tggatgccgc gcgccggagc ggcgagcgcg cgctggcgat cgcccgtcgg ctgggcgatc
     2161 gcatcgagga agcctgctcg ctgcgcctgc tgggactccg cgccgccgaa gccggcgagt
     2221 gggaagaagc ccgcggcttc atcgacgagg ccgcgcggat cctttccggc atcagcaagc
     2281 gctacgagct ggcgaagctc aaccacgccg tgggaatcgt ctggaggaag cgcggacgga
     2341 agggccgctc gcgcggcgtc ctcgacgaat cgatcggata tctccgtcgc gcggccgcgg
     2401 gattcgagac gctccggctg ccggtgcaca cggcgcgggc ccttctcgag ctcgcgcgga
     2461 gcgagctgct ccgcgggttg atggacgatg ccgtcatcca tgtcgaccac gcctcgtcgc
     2521 tgctgacgcc cgaggaagat cccgagctcg cgcgcgatat cgagctcttc cggagcgaag
     2581 tggagcaggg cctgatcgac ggctcctcct cccagaccaa cgagttcgcc gccttcgacg
     2641 agatccggac cgcgcttcgc ggaggcgacg ccgagaccgc gctgcaggag atcctttcga
     2701 tcgtggcccg ccgcgcggga gcgacccgcg gcatcgtggc cgcgccggcc gcgcagggcg
     2761 ggatcgaagc ggtcgcgacc gtcggcatgg cgaaccgcct ggcgcacgat ctgatccgcg
     2821 agctggagcg ccgcatcggc cccgcccggc tcgcttcggg cccgatcgtg accagccggg
     2881 ccggcaccga cgagcgattc cgcgaccttc cggccacgtt ccacatcggc acgcgcacga
     2941 ccgtcgccgt ggttccgctc tcgctgccct cgggcttgcc gggctacctc tatctggacc
     3001 ggtccgagga gccgctcggc gcgttcaagc agcgcgagat caacctcatc accgtgctcg
     3061 cgaaccacgt ggcggtggcg atcctcgagc gccagcgcca gcgcctcgcg cgggagaaca
     3121 gcgcgctccg cggacgcctg ctcgggtcgg ccaacgacca cggcatcgtg acgcgcagcc
     3181 ccgacctcct cgagatcctc tccctgttgg agcgcgtcgg cccgaccgac gcgacgatcc
     3241 tgatcgaagg ggagacggga agcggcaagg ggctgttggc tcgcgcgatt cacgcctcca
     3301 gcgtccgcac caacaagccg ctggtgcagg tgaactgcgc ggcgctgccc gagcagctcc
     3361 tcgagagcga gctcttcggg cacgtccagg gcgccttcac cggcgcggtg cgggacaagg
     3421 cgggtctctt cgtcgaagcg aacggcggca cgctcttcct ggacgaagtg gacaagacca
     3481 cgatcaccat ccagggcaag ttgctccacg tgctcgacaa ccgcgaggtt cgcccggtcg
     3541 gcggcaaccg cccgaccaag gtggacgtgc gggtcatctg cgccacgaac gtgaacctgc
     3601 gcgcgcggat cgcccgcggg gagttcctgg aagacctgta ctaccgactg aacgacatct
     3661 gcttccgcgt gccgccgctg cgcgagcgcc cggaggacat acccatcctg ctcgagcatt
     3721 acatccggcg cttctcggtc gagatgtcca aggaagtggg gggcgtggat cccgagctca
     3781 tccgcacttt ctcctcgctc ccgtggcgcg gcaacgtgcg cgagctggag aaggtcgtga
     3841 agcggatggt cgtcatggcc aacgagggag aagtcctctc gctccgcctg gtcccgcgcg
     3901 acatccttcg caaggaggac gacgtcgccg agggcccggc cacgcttcgc tccgaagtcg
     3961 ccaaggtgga acgccgcatg atcgcgcagg cgctcgagcg ccataactgg aacaagctcc
     4021 aggccgcgcg cgagctctcc ctctcctacc cgaccctcct ccagaagatc aagctcttcc
     4081 acctggaccg gcgcggcacc cggcaaagag cttaaatatt ctgtgtaaac gaatttaata
     4141 accgcaagct tcgtcctccc ttcaagttcc agcagtatcc ccgacacact gttaaatccc
     4201 cctcaaaaac ctgtcaactg ggccctcccc gccgtcctga atctcgtttc cgccacttca
     4261 gcaagttatt attgtacaaa tggttgcgct attttttggg caggtgttga aaatgtcata
     4321 tctggcacga tgggtgcctt agcaagtgat cggagggggg gacctccaaa gagcccttag
     4381 gtcgagccag cgtttgacca tggactagtc gccagaccga gcaccggcct cggtggacgt
     4441 gatgtagccc gaaatgagct ggcacgaccg gggctcaaat tatgttatgg cgactcgcag
     4501 gtcgccataa cccctcggca gtgtccgcgg cactccttcc gatccgagct ttccctgacc
     4561 caccatcgcc cgtcgaaacc caatccgacc taactcctat gtagcgcccc agtcacactg
     4621 ttctgtcttg cccccgtcac acctcgccca atccccatcg cgccaaatcc cgccaccccc
     4681 gcaattccca acgaacacgc cacttacctg acacgccctc gccctcctcg atatggcata
     4741 tgctatgcgg ttccatgatg cggcaaatgg cgccgagatt cgtcggttgc tccagcgata
     4801 cagagctcga ttcttttcac aggaggtgcc cgtgactcgc acgcttcttc tggcgctcgg
     4861 cctcgcggcc gtggcgctca ccgccggctg tcgcaacgag gatcacgtgg tcgggaccag
     4921 caaccctccc ttcgacgtgg agggcgtcta cagcgtcacc ggagacgggc aggtctcgat
     4981 ctactggagg gagaaccagg agaacgacat cgactactac aaggtgtacc gtaacagcgc
     5041 gcccacgggc acgttcacgt ccattggcca ggcggatcat cctcccttca tcgacacgca
     5101 cgtgacgaac ggcacgacct actactatgc cgtgacggcg gtggacacgc gcgggcagga
     5161 gagccccgac ctcagcttcg agaacgtatt cgacacgccg cgccccgagg gcacgaacgt
     5221 gaccttgacc agcgccgcca ccacggcggc cacggccggc tgggacttct cggcgatgtc
     5281 ccgcgtcgct tccaccagcg cgtcggcgga catctggttc gacgcctcga gcggcgcctt
     5341 ggtctacgcc gggaccggcg tgaagatcca ggacgcgggg ctcatcgacc tggtggacgt
     5401 ggacttcggt cctccggcca gcgacggatg gtcggccgac gggatcgtcg aggccattcc
     5461 ggggcacagc tacatcgtcc tgaccgcgga caaccattac gccaagttcg aggtggtctc
     5521 gaaggcggcg ggcagcatcg tgatcgactg ggcctaccag atcgatccca acaatccgga
     5581 gctcgctccg aagattgcga ggaagaactg atgaaacgag cgcgttggcg ccgcctgttc
     5641 gtcgcgggcg ccgcggtcct catggccgcg gcgctgcccg tggcttcccg tgcggcgacc
     5701 cgagtggacg cctcgatctc cacgccccga gtcgacgctt cgatctccta tgaggcggcg
     5761 cattcccccc ggctggacat cgacgtctgg acgaacaagg aggagggcgg cgtctatcgc
     5821 cagggcgagc cgatgcgcgt cctcttccgg cccagcgccg acgcctacgt tctgatctac
     5881 aacatcgaca ccgaaggcta catccacctg gtgtacccct atgacccggg cgatcccgac
     5941 atggtcgagg gagggcgcac ctaccaggtg ccgtcccgca acgatcccta cgatctggtg
     6001 gcggacggcc ccaccggcat cgagtacgtc gtggccatcg cctctccgga gccgtttcgc
     6061 gatctgccgt ggtacctgac cggcggctac gaggggcgtg accgcggcca ggattacggc
     6121 gacgacgatg agggcgattc cggcgtgatc gccggcgacc cctacatcgg catggatcgc
     6181 gtggtgcgcc gcgtggtgcc gcgcggcctg gaggacgagg tcgcgacggc cgacacctac
     6241 ttctacatcg agcggaaggt cgagtatccg cgctacgtct gcgccgactg ccactcctac
     6301 ggcggctggt tcgatcccta tggggacgtc tgttccgtgg tggacatccg gatcgacgcg
     6361 ggctgggcgc actaccgtcc gctccggtcg cggatcctcc ggccgcgcta cttctacatg
     6421 gtccggtcca ccgcgccgac ccgctaccgc cagtggaagg accggtggtc ttcggtcgac
     6481 ggccggacca ctctgcgcac gcgcttccag gtggacggga ccgacaagtt ccagtcgcgg
     6541 cgccagacca tccagcggcg cacgcctccc gagttccagc ggttccgcag cccgcgcgag
     6601 gggcgcgtgt ggcggcccga tgaggtcttg cgccgtggcg gcggcgacaa ccgcgggggc
     6661 gacgtgagag accgcggcga cgttcgcggc gggggcgacc gcggagacgc gggggatacg
     6721 cgccggcgcg aggacatccg tcagaaccag gatcagcggc ggcgtgaatg gatcgagatg
     6781 cgcgagcggc ggcagaacga ccagcgccag aacgaccagc gccagaacga ccagcgccag
     6841 caggaccagc gccagatgcg ggagcggcgc gacgaccagc ggcgcgagga ccagcagagc
     6901 cgtgagcgcg cccgcgccga ggaccgccgc caggagcgcc aggaccggga tcaggcgcgc
     6961 cagcgcgacg acaggagcca ggatcggggc gaccgcggac ggagcgagga gcgccgacgc
     7021 gacgacggcg gacgccgtca ggaccgccgt gaccaagatg gaggcggctg ggggcgccgg
     7081 ggccgctgag ctgttcggct ccatgggcgg gcggcatcgg ggccagcacg cgcctcctcg
     7141 cgtccggact cgttgaccgt ccgcgccggc cgccggtaca ctgacggggt gcgtcgtgcc
     7201 gcgctcccgc tcctcgtcct tgtttccatc gccgtagcgt tcgcgccctc gagatcgcgg
     7261 gccgccagcg ccgtgcctcc cgtgccctgg aagcacgggc cggcgccccg cgagggtttt
     7321 tcgcgcgacc gcctgatcct tccgcctccg ggagcccgcg gcctggcggc gcgcgcgccg
     7381 ctcggcgcac aggccacgat caacatcctc gcgatccggg tcgcgttcag cgacacgccg
     7441 atcgaatcca ccaccgccta ctacgaccgg ctcctcctct tccagaagca gttctgggag
     7501 caagagaccg agggctcgcg gacgctcacg gcgaccctcg tcgattccgt cttcaccctg
     7561 ccgcacccga tggcgtacta cggcgacgac gaccgcttcc aggagcggct ggtcttcatg
     7621 gtgcgcgacc tggtccagct ggccgactcg accgtggact tcgcccccta tcagagcctc
     7681 ctgatcttcc acgccggcaa gggacaggag gcggacgtcc gggacgacag caaggacgag
     7741 atctggtcgg cgttcgtgac gcagcaggac ttccagaccg tccttccgga tacgaccggg
     7801 gcgggtcggg tcgggatccg caccaatgac ctctccggct cgaacccggt gtacatcaag
     7861 gaagcggtcg aggtgccgga gtcggagagc caggacggct tcgtcttcgg gatgaccggg
     7921 gtggtctgcc acgagttcgg ccaccagctg gggcttcccg atctctacga cacgaacggc
     7981 gacgtgggag gtctttccca gggcgtcggc tcctgggacg tcatggggac cggcgtctgg
     8041 aacttcagcg gcttcgcccc ggcggggctc tccgcatggt ccaagatctt cctcggcgtg
     8101 atcgcggcgc agcgggtcgt gaccccccag ccgatctccc tgtccatgct ggagcggcag
     8161 gtcgggtcgc tgccgcgcgc ggtccagatc ccgatgaccg agaccgagta cctcctgctc
     8221 gagaaccgcc gccaggacct gaacgacaac ggacggttcg atttcgacga cgccgacgcc
     8281 gacagcgcgt tcgacttcta catcgactcc tacgcgggcg ccgagttcga cttctacacc
     8341 cccggcgacg gcgcgggatc ggggatcctc gtctaccacg tcgacgactc ggtgatcgcg
     8401 gcggggctcc tcgacaacgt ggtgaactcc aacgccgccc ggaaagggat cgacatcgtc
     8461 gaggcggacg gcatcgagga catggacggg ccgccctcca gctacagcga cgggagtccc
     8521 gacgacgtct tccgcgcggg atggcgcgac aagctcacgc ccgcgaccac gccctccacg
     8581 gccgcctacg gcaacgtgcc gaccgggatc tcgatcacgg gaatcagcgc gcccgacagc
     8641 gtcatgacgt tcgacctctc cttcctgcag gacaagccgg gctggcccaa gctgctcaac
     8701 ggccgtgtcc ggagcgcgcc ctccctggcc gcggacgtgg acggggccgg cccgccgttc
     8761 acgcaggagc tgctcgtccc ggtgcagcgg ctcaacaata ccggggcgct ctacatcttc
     8821 cgccccgacg ggaacgactt cctggacggc gacgccaccc ccacaccctt cgcgagcacg
     8881 acgtcggggg tggtcaccag cccctgcgtc ggcgacgtgg acggggctcc ggggaacgag
     8941 atcgtcttct cgacggcgaa cgggaccctc tacgccttcc acgcgaacgg caccgaagtg
     9001 gtggacgggg acaacaaccc cctcacgctg ggcgtcgtga cggcgggctt cgcgacgggg
     9061 gtgcgcgcgc agccggtcct ggccgaggtg gacggcacgc cggggctcga gatcgtgatc
     9121 ggctcgccgg cgaccgcgac gggctactcc ttcgtgcgtg tgatccggct tgccggcggc
     9181 gtcctggagg agcattcgat gctggtcggc ggtagctccg aagcgcccgc ggtcgcgacg
     9241 ggtctcgaag ggggcgcgaa ccaggtggtc atcgccgcga ggcgcacggt gatcggcgag
     9301 aactcgcttc cgggcctcta tctcctgaac tgggagatcc tgaccgaccc cgttcgcgac
     9361 cccctcgatc ccgagaacat cccggggacc cgcctcgccg ccgggcagtt ctccgcgccc
     9421 gtggcggccg acctggaagg gaacggcaag cacgagatcg tggtcgcgga cacgaccggt
     9481 ctcttccacg cctatcgcgt cgcgatcggc gcccacattc cgggcgacga tccggccggg
     9541 tacgtcagca ccaccgagct cccgggatgg ccgacgccgt tcccgggtgt ctcgcgcggg
     9601 cgcgcctcgg aggtctcggt cgccgatctg gagcgcgacg gacatcccga ggtgttccag
     9661 accggcgaga acgcgcgggt ggccgccttc tactggacgg gcgcgccgcg ggccggctac
     9721 ccgctgcgcc tggccgcccc cggcgcgccg gccgattcgg cgggctcctg ggctccgatc
     9781 gtcgccgacg tggacggcga cgggctccgg gacgtgatcc cgatccttcc cgacggccgg
     9841 cgcctcgcct tccggggcga cggctcgccg atcggcggct tcgccgagct ggggagcacc
     9901 ggcctctcgg cgccgcccat cctcgccgat ctggacggcg acgggcccgc cgagtggatc
     9961 gagaccttcg acgcctcgtc gcaggcgcag atcgacgtga agatccccgt ccttccgatc
    10021 gccgcggcct cgatcggctg ggggcagtac cggctgagcc cgacccggaa cgcggtggtg
    10081 gacgccgtca ccggagggcc cgccccgggg acccaggggc tgtccgaagt gtatgtctat
    10141 ccgaatcctt cccgcgacgg aacgagccgg gtccactacc gcctggaagc ggcggcgacg
    10201 tcggtttcga tccggatcta cgacacctcc ggctcgctcg tcgccgacct ctccacgggc
    10261 gcggcggacc ggctgggctc ttcggaacat tccgtggcgt ggaacaatgg ctcgatcgcg
    10321 agcggcgtct atgtctgccg ggtcgaggta caatcaggcg gccggaccga ggtgcgcttc
    10381 gccaacctcg ccatcgtccg atgagccgtc ccctggagaa ccgcatgcgc ccgacctgcc
    10441 gtggcatcgc agccgccttc gcgtcctgga tcgtcctcgc gctcgccccg cacgcggccc
    10501 tggccgcggc cggcgagacc ggattcgcct ccctgaagct gggcgtgggc gcccggccga
    10561 tgggcatggg cagcgcctac gtggcgctgg tggacgaccc gacggccacc tactggaacc
    10621 cggcggggct ggccgcggtc cgctcgaccc agatcacggc catgcacaac gagtggatcc
    10681 aggatttccg ccaggaatac gcggcggtcg cagcaccgct cggaaagggc accctgggct
    10741 tcgccttctc cggcttctac acgtcggagc tggagggtcg cgacgaggtg ggcaacgtca
    10801 cccacaactt cggattcaac gacatcacca tgacggccgg ctacggccgc tccttggccc
    10861 cgggcctcga tgccgggctc gcggtgcgct atctgcgcga gatgatcgac gaccaggacg
    10921 gggcgacgtt cgcggccgac ctgggggcgc gctatcgcgt gaaggagaca gggctctcgt
    10981 tcggcgccgc ggcgcagaac ttcggcggca agccgaagct ggtggaccag tcgttcaagc
    11041 tgccggccac gctccgcctg ggcgccgcgt ggtcgaagcc gatcgccgcg ctccattcga
    11101 ccggaacgct cacgaccgag atccggaaag cccgcgcgga ggacgcgcga ttccacgtgg
    11161 gaggggagct ctcctacaag gaccggctgg cgctgcgggt gggggggaag ttcgggtacg
    11221 acgcggaaga cgtttccttc ggcatcgggc tgacccagag caagctccgg ttcgactacg
    11281 cgctggtgcc gctgtcgtcc gatctgggca ccgcgcactt cttctcgctg acggccaggc
    11341 tgtaggacgc ccggcgcccg gcgcccgccg ggtccgacga ttgtagaagt gcgcagcgac
    11401 actccgccgc gcgtgtggta tgagatcgat catccgccga attcgaacat cgagaacacg
    11461 ccgcgctcca gcttcaccac cgtcctccgc tcgatccagt cgccgaggac gatgaaatcg
    11521 cggccgttcg cgcgctggtg gatggggcgg tggaaatggg cgaggaggac cgcgtcgaac
    11581 ccctcggcaa aacgcggctc ggcgagcagg gcctgcatct ccgcttcggt gcgcttgggg
    11641 cgcggggcgt ggtggcggct ggtgtgcgac gtggcgctgg cgatgggaat gccgatgtcc
    11701 ggatggatcc agcgaaacag gaactgggcc gcgcggtttc ggagcacccg cttcagcagc
    11761 ttgtatccgt gatctccggg gcccaatccg tcgccgtggg ccaggaagat cttccgtccc
    11821 tggagggcca gcgccatcgg ctggtcggta aaggccaccc cgagctcgtt ccgcagataa
    11881 tcaccgatcc agaaatcgtg atttccgccg agatacgtaa cagggacgcc gctccgggcg
    11941 agggagccaa gcgccgtgag gacgccgacg aaccgcttcg ggatggcgtg gccgtactcg
    12001 aaccagaagt cgaagaggtc gcccaccacg taaagctccg cggcttcgga cgccacgcgg
    12061 tcgaaaaacg cgagcagatc gcgctcgcgg gcgcggctgg aatcgagggg gccctggccc
    12121 aagtgggcat cggcgacgaa ataggcgcaa ccccggtcgg gcatggagcg tcagtataag
    12181 caggttcccg gccgtgcccc atcctgttcc acgggatccg gtagccgccg ggaactgcac
    12241 tggttaggtc gggtactacc ggcttgacag gggagtggca agccccttcg cttcagcccc
    12301 agaacgcatt gctcaaagga ggtttatggc gccgcagacg ggtttttggg accggctgca
    12361 ggacgaggtc cgcaccggct acgaacgcac ccgccgcacg accacgcgga cggttcggat
    12421 cggtgtcctt cgggtggatc tcctgtccct gcgccgtgac cgcacccgcg ctctctcgca
    12481 gctgggggag cgcgcgttcc agctctggaa cgggggatcg ctcgcggtcc tggacggcga
    12541 cgaggaggcc cttcgcctca agtcgctggt cgaaggcatc gagcgatgca ttgcggacaa
    12601 ggaggcggag cttcaatcat tgaggaagga agcggacgag gcggacgcgg ggcgtcccgc
    12661 cgagaacgct tccggcggcg cggcgcccgg gacgaccccg gacgatccgc ccgccgagcg
    12721 tcagaccggg ttcacggagt cgggcggccc gcggccgccg cggatggagg gaatgaatgg
    12781 aatcacgtga aacggtccgc gccgcgaagg tgctcctcgt cgcgggcgga ctcctgctca
    12841 tcggcatcgt cgtcggattc atcctcgcgg gcgggagcaa ctcctcgccg gtgctccagg
    12901 ccaaggagag cgtggtttcg accgggctcg agagtccgtt cacggcgatt gccgaccgga
    12961 cgctccccgg agtcgtgagc atcgaaacga agcgcaacgt caccggatcg gacggcccct
    13021 cggcgcagtt cgagggcccc tacggcgact tcttccgccg cctcttcccc gagaccccgc
    13081 agcagcctca gcagcagcgc cggaacatcc ggatcccgag cagcggctcg ggcttcatca
    13141 tcgatcacgc cggctacatc ctgaccaata accacgtggt tcgggacgcg gccgagatca
    13201 cggtgacgct gaacgaccac cgcaagttca aggcgagggt cgtcggcacc gatccgggca
    13261 ccgacgtcgc ggtcatcaag atcgtgggcg ccggcgacct gccggtcgtg ccgctgggcg
    13321 actccgacga gatccggatc ggcgactggg cgatcgcgat cggcaacgcg ctgggcgagc
    13381 tggacggcac gctcaccgtg ggcgtgatca gcgccaaggg ccgcacgaac ctggccatcc
    13441 agggcggcgc tcccagctac cagaacttca tccagaccga cgcctcgatc aacttcggaa
    13501 acagcggcgg tccgctcttg aacatccgcg gcgaggcgat cggcatcaac acggccgtca
    13561 atccgtcggg cgagggaatc gggttcgcca ttccgatcaa catcgcgagc agcatctcga
    13621 agcagctgat cgccaccggc aaggtgacgc gcggctacct gggcatcctg ccccaggagc
    13681 tgacccccga gctggccgac aactggggca tccagggaca cggcgtgctg gtcggcggcg
    13741 tgagcacgaa cactccggcg tcgcaggccg gcttccaggt caaggacgtg atcaccgact
    13801 tcgacggcaa gcccgtggac gacgtgcagc gcttccgcct gctcgtcgcc gagaccccgg
    13861 tgaacaagaa ggtcgccgtg cgcgtgctcc ggaacggcaa gccgcgcgac ctcgcggtca
    13921 cgctggcgga gcgtccgccc gaggatcagg tcgccaaggc cgacaacgcg attcccaact
    13981 ccacgtcgga ttggctcggc gtccaggtct ccgcgctggg cagcgacgcg gcgggcgagt
    14041 tcaacctgca cgacaagacc ggcgtcgtga tcacggacgt caccgagggc agcggcgccg
    14101 acgactcggg cctgcgtccg ggggacgtga tcaaagaagt gaacgacatc ccggtcaagg
    14161 atatggtgga atacacccac gcggtcgaag ccgcgaagcg caagaacccg gcgaagccgg
    14221 tggtcttcct gatcaagcga ggcgatgcga cccaattcgt ggccgtcgag ccctctcagg
    14281 attaacggct cgaacgcgcg cccggtgcgc gagcgttcga cgaggcccgc agcgcgctcc
    14341 cttcgggggg cgcgggcggg cctcggccgt gttgcgccgc tcgcgttcct cctgctcctg
    14401 gccgtggggt gcttcctggg ggccgcgccc gcggccacca gcgcggcgcc cgcgccggcg
    14461 gcgccggagc tggtgcggct ccgcttctgg acggctcccg agcacacccg cctcgtcttc
    14521 gacctgagcg gcgcggcgcc ggcggccacc aagttccgga tgatcgactc gaccacgttc
    14581 gaggtcttca tgccgcgggt gcggaagtcc cccgccgtga cgaccgaatt cgtcggggac
    14641 agcctcgtgg ccgacatctt cccggccgtc tccgactcgg gcacgtcggt gcgcatccat
    14701 ctgaagaaga aggtgacccc gctcgggttc gtcctcgcgg ccgccgacgg cgttcccgac
    14761 cgcgtggtcg tggacgtgcc gatgccggtc aatccggccg ccgagcaggc gctccagcag
    14821 aaggtggccg agctgaagag cggaaagaag atgttcatcg tggcgatcga cgcgggacac
    14881 ggcggcgagg acacgggcgc gatcggctac cggcatctcc aagaggccga catcacgctg
    14941 gccgtggccc ggaagctgaa atcccagctg gacctgatcc ccggcatcag cgcggtgctc
    15001 acccggaaca gcgactactt catcccgttg cggaagcgga tcgatctcgc gcggaaatac
    15061 caggccgacc tctttgtcag catccattgc aacgccagcc ggaaccacga cgcgaccggc
    15121 accgagatct acttcctctc cctgaacgga gcgaccgacg aggcctcgcg ctccctggcc
    15181 gagaaggaga acgccgcgga catgatcggc ggcgtgcccc ccgagagcgg cgacgacctg
    15241 ctctccatcc tgttcgacct ccgacagaac gacacggtcc gccgctcgag cgagctggcg
    15301 gagctcctga tcgacgccgt gagcgccgac acccgcctga gcacgcgcgg ggtgaagcag
    15361 gcgggcttcg tggtgctgaa ggcgccggag atcccctcgg tgctcgtcga gacggccttc
    15421 atcacgaatc cgcgggaggc ggcgatcctc aaggacacgc agtttcagaa caaattcgcg
    15481 gagcacctcg cccaggggat ccagagctac aaggccatgc gcgaccgggc ctccggtgga
    15541 gcggcggccc cgacaggcag ctcgaactag cgggcgaacg ccggaattca ggctggacac
    15601 acttcgaagc cggtgtactc ttgatcccgt atcgtttcgg acccatccct gaaggagaca
    15661 cacccatgaa tcgcatcctc agcaaatatg cacctcttgc cgcggccgcg ctcctcgtgg
    15721 ccgggtccgc gttcgccggc ggtgcttcct gcgggagcgg cgacgccccg cggtcggctt
    15781 cggccgggca ccactgcgaa ggcatgaagg ccgagggcgc gtcggcgatg tcctgcccga
    15841 ccgtcaaggc cggacagaac atctactcgt tcgccgttcc gggcgccgag tgcgagaatt
    15901 gcgtcaagtc gatccagacg gcgctgatgg cccagaaggg cgtcgcctgc gcccacgtgg
    15961 atctcacgag ccacaccgcc tacgtcatcg cggaccgctc cttcgaccgg aacgcgatcg
    16021 ccaagtgcat ccagaccgcc ggcttcaaga acaagtacaa ggggcagggc gcgaaggtgc
    16081 aggccgagtt cgccaaggcg atgagcgcgc cctcgggcaa ggcgtcggcg tgtccgtaca
    16141 aggagagcaa ggaaagcaag gagaaggaca agatctgaac cggcccggcg ccgtctttgc
    16201 gctgagtctt ttcttcgggg cgtgcctgtc ttcgggcacg ccccgtttcg ctgccgcttc
    16261 cgccgagtcg ccgatcgact gggaagccca tctcgacctg gtccaggtct cccgcaccga
    16321 ccagatcaat tcgaacttct tcagcttcgc cgaaaacccg ttccacgcga tccggctccg
    16381 cctgctcgcc gacggccgga tccaggagca catctccgcc ttcgcggaga tcctctccga
    16441 cgacgggaga gcgccccgcc tctacggcgg attcgtgcgc ttctccgacc ccaaggggcg
    16501 cgacatccat ctcgaggcgg gaaagattcc gatgcacgtc ggcgcctttc ccaaccggtc
    16561 ggacgcctca cggaacaacc tgatcggctc cccgctcgtc taccagtacc acctggacgt
    16621 ccgcgacgac caggtgccgg tgcgtcccga ggacatcgtc gccaaccagg ggcgaggcta
    16681 cttcacgaac tacgcggccg gcggcctcac cgggctcggc taccccggaa cggggcaggc
    16741 gctctcggtg ctctacgaga attgctggga cgtcggcgcg gtcgtggtcg gcaccgcgag
    16801 acccttcgaa ttcgcgctcg gcgtcaccaa cggcacggtc ggatccatgg tcagcaccga
    16861 cgacaacgac ggcaagcagg tcctcggccg cgtgggcctg gctcccgcgc cctgggtgcg
    16921 cgccggggtc tcgggggcgc gcgggcccta tctgagccgc gcgctcgaag agagcgcgct
    16981 gccggcacgg aagagcgcgg acgactacaa ccaggtgttc gccggaggcg atctggagct
    17041 ctcctacgat cgcgcggtcg tctacggcga gtacgtctgg aaccgcttcg agcagccgtg
    17101 ggtgggcgac ctggatctcc gcgcctggta cgcggagggg aaggtgacgg tgctccccgg
    17161 ctggtacgtg gcgggccgct acagccgcgt cgcgttcgac aatctcacgc tcgccggcgg
    17221 ccgggccgcc gcctgggacg ctcccatctg gcggcgcgag atcggcatcg gattcaaacc
    17281 ctcgaagatg ctcctcgcca agctggtcca tcaggagacg cacaccgaga ccgtgccgcg
    17341 gcgcgtggag gccttcatgg ccgcccagct cgcggtggtc ttctagccca ccgttcctcc
    17401 agggaggttc gcgatgcgct ttctcgggaa gtacgcggag accctgttcg cgatcatgcg
    17461 gatcgtggtc ggattcctgt acctctgcca cggcgggcag aagctcttcg gctggttcgg
    17521 cgcggagaag gcggcggagc cgctcatgat cgcggcgggc gtcatcgagt tcggtggcgg
    17581 gctcttggtc gcgatcgggc tcgtcgcgtc cgcggccgcg ttcatcgcga gcggcgagat
    17641 ggcggcggcc tacttcatgg tccacgcgcc ccactccttc tggccgatcg tgaaccacgg
    17701 cgagctgccg gccttgtact gcttcgtctt cctgtacatt gcggcccacg gctcgggccg
    17761 gctgagcgtg gacgcggccc ggcggggggc tacccgtacg taggtccact cgccccgcgg
    17821 cccggcggcc gggaatacgc ggttgttgcg gctatacaac ccccgtagac tgatgggagc
    17881 ggccctctac gccgatcgcg cggggggacg cgccagaaac cgcgatgatc acgcccgagc
    17941 catcgcgcgc cggggccatc ccggagcgct ccttcgatag cgagtcacct ctcgacgaca
    18001 cggtattccg ccaaatcgtc cagggagcgc gggactacgc cattttcctc ctcagccccg
    18061 aggggtacat ccgcacctgg aacctggggg ccgagcggat caagggatac accccatcgg
    18121 aaatcatcgg ccagcacttc tcccgctttt acacgcagga cgccatcgac cgcggatggc
    18181 cgatgcgcga gctggagatc gccaaggccg agggccgctt cgaggacgag gggtggcgcc
    18241 tccgcaagga cggatcccgc ttctgggcga acgtcatcat caccgccatc cacgacgaga
    18301 gcggcgagct ggccggcttc tccaagatca cgcgcgatct gagcgagcgg cggcgccacg
    18361 aggagtcgct gcgcgagagc gaggagcgtt tccgcctcct gctggaggga gtctccgact
    18421 acgccatcgt gttcctgaac ccggaagggt acgtgagcag ctggaacgtg ggcgcgcggc
    18481 ggatccaggg ctacggggcg gacgagatca tcggccgcca tttctccctc ttcttcccca
    18541 gcgaagccaa gagcgcgggc gcgccgcagc tcctcctgga tcaggcgcgc gccaccggcc
    18601 gcgccgagga cgacgggtgg cgcacccggc gtgacggctc gcgtttctgg gccaacgagg
    18661 tgatcacggc gctgtacgac cacgagggat ccctgcgcgg gtacgccaag gtgacgcagg
    18721 acatgaccca gcgccggaag atcgaggtgc tggaggagac cgcgcgccgc gtgaacgaat
    18781 tcctcgccat gctcgcccat gagctgcgga atccgctcgc gccgatccgg aacgccgtgg
    18841 gactcatgcg cgcgaagcgg atcgacgatc ccacgctcca gtggtcgcgc gacgtgatcg
    18901 agcggcagac cgcccacctc tcgcgcctgg tcgacgatct cctcgacgtc agccggatca
    18961 ccagcggccg cctcacgctg cagcgcgagc gtctggacat ggccgtcgtc ttcgagcggg
    19021 cggtggaggc cagccggccc ctgatcgagg ggcggcgcca ggcgctggag gtgcggctgc
    19081 ccgagacccc gctctgggtg gaaggggacc tgatccggct cgcccagatc gtgctcaatc
    19141 tcctgaacaa cgcggccaag tacacgccgg ccggcgggcg catctggctg agcggagagc
    19201 tggaggccgg aaaggccgtc atccgcgtcc gcgattccgg agtggggatc tcgtcggagc
    19261 tcaagacccg gatcttcgat ctcttcacgc agggggcgcg cacgctcgac cgctcggacg
    19321 gcggtctcgg catcgggctc acgctggtcg agcgactcgc ggcgctccac ggcggcaccg
    19381 tggaggtggt gagcgccggc gcgaaccagg gcagcgagtt catcgtgcgg cttccggcgg
    19441 ccgccccgcg cgaggccgcc ccgggcaagg acgccgcggg agcggggtcg ggccacgccg
    19501 cgcggcgtcg cgtgctcgtg gtggacgaca accgcgacgc caccgagacc atgtcgatgc
    19561 tcctgggcgt ctggggacac gacacgcgga gcgcgaacga cggacccggg gccctcacgg
    19621 tcgccgcggc gttccagccc gaggtggtcc tgctcgacat cggactcccc ggcatggacg
    19681 gctacgaggt cgcgcgccag atgcacgcgc tgcccggtct tcggaacgcg gtcctcatcg
    19741 cgatgaccgg ctacggccag gaagaagacc gcatgcgctc ccggtccgcg ggattcgccc
    19801 gccacctcgt gaagcccgcc gacccgagcg ccctgcggca gctgctcgag tcgcttccgg
    19861 agtagcccgc cgggcggctg ggccgctcag cccgcgaggc gcttggccag cgcgcggcgg
    19921 ctggcggggg tgttgtcgaa ggtcgcgagc agcgtgcgca cctcgggcca ctcgtcggtc
    19981 acgtgcgggt tgagcgaggc gaccgtc
//
LOCUS       DASSLZ010000043         3306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_775503, whole genome shotgun sequence.
ACCESSION   DASSLZ010000043 DASSLZ010000000
VERSION     DASSLZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775503"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFM00_01525"
     CDS             <1..487
                     /locus_tag="VFM00_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496955.1"
                     /translation="QMELDGLKVEVQKQQDLLATLEREAASSRISEAMETSQLSFRIE
                     VVEPPVLPLKPVWPDKLKVILGALLLGPLFSVGIILGAERVGAILRTVEQAEEEMGTK
                     VIGTIPRIEGWSRPGSFLENNWAPLSILAILILTALVSVTYTSLNSTRHAATTSGELR
                     R"
     gene            495..1331
                     /locus_tag="VFM00_01530"
     CDS             495..1331
                     /locus_tag="VFM00_01530"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HET7496956.1"
                     /translation="MPKEAPRSLFTEFSTDAPYVTEARRLLQSLMRQHRGPACRVYMV
                     SSAGRGEGKSTICGLLGIVAARIFHRHTLIIDADMRRPTMHQLLDLSQKPGLYELLHG
                     TASLDLARRPTTLPLLHAIPSGKPGSHVSDSYDDEAFEKLLQSVRPSYDLIFIDAAPI
                     VPVVEPIMMAEHVDGILLVAMAGRTPITMIRRMRGILTPVASKVAGAIVNNATEGLPY
                     YYDYSYYGYKPLLSRRDRNKVGGPTAPGAEVQPNGDGAATPHAASSPAPPAHAAKPKP
                     KE"
     gene            1337..2665
                     /locus_tag="VFM00_01535"
     CDS             1337..2665
                     /locus_tag="VFM00_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET7496957.1"
                     /translation="MSPRKRSGTAASRLSARLDNRSARIGVIGLGYVGLPLAVEFAKV
                     GYRVTGFEIDAKRAATLNRGISYIQDVPTGDVRGLVREGMLQATLDFDALAKMDVVDI
                     CVPTPLRKTKDPDVSYIVAAVKDIAPRLKRGQLVVLESTTYPGTTDELVLPLLAEGGL
                     TVGKDFFLAFSPERVDPGNEKYQTKNIPKVVGGVTPECTRLVAQFYGAVMDSIVPVGS
                     TRVAEMVKLLENTFRSVNIGLVNELALMCDRMGIDVWEVIDAAKTKPFGFMPFYPGPG
                     LGGHCIPIDPFYLSWKARESGFEARFIELAGNVNGGMPHHVTKRVSEALNSVRKPLRG
                     SKVLVVGVAYKADIDDVRESPSLDIMEILERDGARVTYADPYVPTLRHNGSMLHAKPL
                     TPAAIRAADCVVIATAHKNLDYAMIARNAKTIVDSRNALKGRRTRGVFKL"
     gene            2674..>3306
                     /locus_tag="VFM00_01540"
     CDS             2674..>3306
                     /locus_tag="VFM00_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7496958.1"
                     /translation="MGRYLVTGGAGFIGSHIAERLLRDGHEVRILDNLATGKRENVEA
                     IRPAGPGRLEWLEEDVRDLAACRRACAGVEYVLHEAALGSVPRSVENPGETTAVNVLG
                     TVNVFQAAREAKVRRVVWASSSSVYGDTPTLPKHEQMPLSPRSPYAAGKLAGEEFARV
                     FAGTMDLPVVSLRYFNVFGPRQDPDSQYAAVIPRFIRALVEGKRPTVYGDG"
BASE COUNT          545 a         1175 c         1096 g          490 t
ORIGIN      
        1 ccagatggag ctggacggac tgaaggtcga agtgcagaag cagcaggacc tcctcgccac
       61 cctcgagcgg gaagccgcct cctcgcgcat cagcgaggcg atggagacga gccagctctc
      121 cttccggatc gaagtcgtcg agccccccgt gctgccgctc aagccggtgt ggccggacaa
      181 gctcaaggtg atcctgggcg cgctcctcct ggggccgctc ttctcggtcg gtatcatcct
      241 cggcgccgag cgcgtcgggg cgatcctccg cacggtggag caggccgaag aggagatggg
      301 gaccaaggtg atcgggacga tcccgcgcat cgagggctgg tcccgccccg gctccttcct
      361 cgagaacaac tgggcgccgc tctccatcct ggccatcctg atcctgaccg cgttggtgtc
      421 cgtgacgtac acgagcctca actcgacccg ccacgccgcg acgacgagcg gcgagctgcg
      481 ccggtagggt tcccgtgccc aaagaagccc cgcgcagcct cttcaccgag ttcagcaccg
      541 acgcgcccta cgtcaccgag gcgcgccgtc tcctccagtc gctgatgcgc cagcaccgcg
      601 ggcccgcctg ccgcgtctac atggtctcca gcgcggggcg cggcgaggga aagagcacga
      661 tctgcgggct gctcggcatc gtcgcggcgc ggatcttcca tcgccacacg ctcatcatcg
      721 acgccgacat gcgccggccc acgatgcacc agctcctcga cctctcgcag aagccgggtc
      781 tgtacgagct gttgcacggc acggccagcc tggacctggc gcgccgcccg acgacgctgc
      841 cgctcttgca cgcgatcccg agcggcaagc ccggctcgca cgtgagcgat tcctacgacg
      901 acgaagcgtt cgagaagctg ctgcagtcgg tgcgtcccag ctacgatctg atcttcatcg
      961 acgcggcgcc catcgtgccg gtcgtggagc cgatcatgat ggcggagcac gtggacggga
     1021 tcctgctggt cgccatggcc ggccgcaccc cgatcacgat gatccggcgc atgcggggca
     1081 tcctgacgcc cgtcgcgagc aaggtggccg gcgcgatcgt gaacaacgcc acggaaggcc
     1141 tgccgtacta ttacgactac agctactacg gatacaaacc gctcctgtcc cggcgcgatc
     1201 ggaataaggt ggggggtccg accgctcccg gcgccgaggt ccagcccaac ggcgatggag
     1261 ccgcaacacc ccacgcggcg tcttcccccg cgccaccggc gcacgccgcg aaacccaagc
     1321 ccaaggagta accctcatgt cgccccgcaa gcggtccgga acggccgcat cgcgtctttc
     1381 cgctcgccta gacaaccgta gcgcacgcat cggcgtcatc ggcctcggct acgtcggcct
     1441 gccgttggcg gtggaattcg ccaaggtggg ctaccgcgtc acgggtttcg aaatcgatgc
     1501 caagcgcgcc gccacgctga accggggaat ctcctacatc caggacgtcc cgaccggcga
     1561 cgtgcgcggc ctggtgcgcg aagggatgct gcaggccacg ctcgacttcg acgccctcgc
     1621 gaagatggac gtcgtcgaca tctgcgtgcc cacgccgctt cgcaagacga aggatcccga
     1681 cgtctcctac atcgtggcgg ccgtgaagga catcgcgccg cgcctgaagc gcgggcagct
     1741 ggtcgtgctg gagagcacca cctatcccgg caccaccgac gagctggtgc tcccgctgct
     1801 ggccgagggg ggcctcacgg tggggaagga cttcttcctc gccttctcgc cggagcgcgt
     1861 cgacccgggc aacgagaagt accagaccaa gaacatcccc aaggtcgtgg gcggcgtcac
     1921 gcccgagtgc acgcggctcg tcgcgcagtt ctacggggcc gtcatggatt cgatcgtccc
     1981 ggtgggctcc acgcgcgtcg ccgagatggt gaagctgctc gagaacacct tccgcagcgt
     2041 gaacatcggg ctcgtgaacg agctcgcgct catgtgcgac cggatgggaa tcgacgtctg
     2101 ggaggtcatc gacgccgcga agacgaagcc cttcgggttc atgccgttct acccgggacc
     2161 gggactgggg ggccactgca ttccgatcga tcccttctat ctctcctgga aggcgcgcga
     2221 gagcgggttc gaggcgcgct tcatcgagct cgcggggaac gtgaacggcg ggatgccgca
     2281 ccacgtcacc aagcgcgtga gcgaggccct gaactccgtg cggaagccgc tccggggctc
     2341 gaaggtgctc gtcgtgggcg tggcctacaa ggccgacatc gacgacgtgc gcgagtcccc
     2401 ctcgctcgac atcatggaga tcctcgagcg cgacggggcg cgcgtgacct acgccgatcc
     2461 ctacgtgccc acgctgcggc acaacggctc gatgctccac gccaagccgc tcacgccggc
     2521 cgccatccgg gccgccgact gcgtcgtgat cgcgacggcg cacaagaacc tcgactacgc
     2581 catgatcgcc cggaacgcca agacgatcgt ggattcccga aacgccctca agggacgacg
     2641 cacgcgcggc gtcttcaagc tgtagggggg cccatgggac gctacctggt caccggcggg
     2701 gccggattca tcggatccca tatcgccgag cgtcttctcc gggacggcca cgaggtccgc
     2761 atcctggaca acctggccac ggggaagcgc gagaacgtgg aggcgatccg tcccgccggt
     2821 cccgggcggc tggaatggct ggaggaggac gtgcgcgacc tggccgcgtg ccggcgcgcg
     2881 tgcgccggcg tcgagtacgt cctccacgag gccgcgctcg gctcggtgcc gcgctcggtc
     2941 gagaacccgg gcgagaccac cgcggtcaac gtcctcggca ccgtgaacgt cttccaggcg
     3001 gcgcgcgagg ccaaggtgcg ccgcgtggtc tgggcgtcgt cctcctcggt gtacggcgac
     3061 acgccgaccc tccccaagca cgagcagatg ccgctctccc cgcgctcgcc gtacgcggcg
     3121 gggaagctcg ccggggagga gttcgcgcgg gtcttcgcgg gaacgatgga cctgccggtg
     3181 gtctcgctcc gctacttcaa cgtgttcgga ccgcgccagg atcccgactc gcagtacgcg
     3241 gcggtgatcc cgcgcttcat ccgcgccctc gtggaaggga agcgcccgac ggtctacggc
     3301 gacggc
//
LOCUS       DASSLZ010000044         8790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1788721, whole genome shotgun sequence.
ACCESSION   DASSLZ010000044 DASSLZ010000000
VERSION     DASSLZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788721"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFM00_01545"
     CDS             complement(<1..292)
                     /locus_tag="VFM00_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496959.1"
                     /translation="MNRSLSILAVTLFALLTILPALALADDMPPAEHKSIGLGFHDVD
                     APVGVRWWLAGQKVGIDLGVGFNSKSATLDGYPDDNLTSWAVGAGIPIVVKSW"
     gene            complement(406..558)
                     /locus_tag="VFM00_01550"
     CDS             complement(406..558)
                     /locus_tag="VFM00_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HET7496960.1"
                     /translation="MIETIAVLLVVMWLLGMLTHYTLGGLIHILLVLAVIAIIIRLVS
                     GRRPVV"
     gene            complement(758..1234)
                     /locus_tag="VFM00_01555"
     CDS             complement(758..1234)
                     /locus_tag="VFM00_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HET7496961.1"
                     /translation="MLTFEDALRRRAASRVLSQTFVFTNGCFDILHPGHVALLAHAKR
                     QGHYLLVGLNSDRSVHALKGRGRPVQSEADRAAILAALTDVDGVFVFDDETPRSVIEA
                     LQPDVLVKGGDYKPEDVVGRETVEAAGGRVDIFPTVAEHSSTRLLSKQHAEMTRRV"
     gene            complement(1236..2276)
                     /gene="rfaE1"
                     /locus_tag="VFM00_01560"
     CDS             complement(1236..2276)
                     /gene="rfaE1"
                     /locus_tag="VFM00_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HET7496962.1"
                     /translation="MKPLPSAPPGAVPVPSRGPGGALAARVRAMAGRTVLVVGDVILD
                     RYLWGRASRVSPEAPVLVVDVEREELRLGGAANVAHNVRALGATPILCGLLGADAAGE
                     ECARLLAERRVEPETGVVKDAARRTTLKTRIIAHHQQVLRADEESREPAGAAAQEALW
                     ERIRAAAGRADAAIVSDYGKGVVTPELLDRLLPELSRRGVPVAVDPKEEHFFRYRGVT
                     VITPNVAEASQAWGRKLRDTESLLEAGFGLRERLDARSVLITRGEEGMSLFTAEGHTH
                     FPTRAREVYDVTGAGDTVVAAMGAALAAEATLPEACVLANHAAGLVVAHVGTAAAGAA
                     ELIASLEEPALG"
     gene            complement(2273..2602)
                     /locus_tag="VFM00_01565"
     CDS             complement(2273..2602)
                     /locus_tag="VFM00_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496963.1"
                     /translation="MTERRKHDEDELEPDKIPEDLVPVHDVPDEATGTMLADFLREQG
                     VLAMLQPVEISMLPGVESVGHGYWGKIEVLERDEAKAKALIRDYLASRPRTTTRRKVD
                     IEGADEA"
     gene            complement(2599..3582)
                     /gene="galE"
                     /locus_tag="VFM00_01570"
     CDS             complement(2599..3582)
                     /gene="galE"
                     /locus_tag="VFM00_01570"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET7496964.1"
                     /translation="MNALVVGGAGYIGSIVAEELVRARHSTIILDELSTGHRAAVPPL
                     AFLVAGAIQDPERLREAFTTKEIDCVIHLAAKSIVSESVAHPDLYERANVEYGKILLD
                     AMRDHGVKHMVFSSTAAVYDAAAPMPLREDSPLKPGNPYGETKLRFERMLRERGETDG
                     LRYVALRYFNVAGASIDRGEDHRNETHLIPLVIDAARGAKGPVPVYGSDYPTEDGTAI
                     RDYIHVVDIADAHLRAMRYLADGGESVTLNLGTGTGSSVRQVIRTVEEVTGLPVPTQD
                     SPRRAGDPPVLIASAAEAWRVLGWTRQFEELNEMVETAWQWRQRFPEGYEE"
     gene            complement(3579..4292)
                     /gene="rpe"
                     /locus_tag="VFM00_01575"
     CDS             complement(3579..4292)
                     /gene="rpe"
                     /locus_tag="VFM00_01575"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013145788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET7496965.1"
                     /translation="MKGAPEARGAAGTWSAPGRCRVCPSILSADFARLAEAIASVEAA
                     GADFIHVDVMDGQFVPNLTFGPIVVAGIRKMTRLPLDVHLMIVDPLRHLPAFADAGAD
                     HVIVHVEAVDDPAAAARAIRARGLRAGLSIKPATPMERLIPALPDCDIALVMTVEPGA
                     GGQAFLPGSPERIAATRRAVAERGLDCLVMVDGGIDVHTAPLAGRAGADAFVAGHSIF
                     HAPDPPAAVRALRASLARP"
     gene            complement(4289..5245)
                     /locus_tag="VFM00_01580"
     CDS             complement(4289..5245)
                     /locus_tag="VFM00_01580"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET7496966.1"
                     /translation="MRGRRRPVEPWRRRAIAQREAERVREAEPGVSLGTAPETKLAEP
                     PPSGDGGRAIDASPLALDSLSPEATAPPRAERRRGPRFNLITGTLLLSAIALLGGFLV
                     INLVLMPSFTRQGAEVPVPEVTGLSEREAERVLASEDLRLSKISEQWSPDVPRGFIAA
                     QDPPAGGQVKRGRRIAVVVSLGAQGTSVPVLDGVSERQAGIQLESSGLRRGKVARVYT
                     DEIGKDLVIASDPPGETVVEQETVVDLLVSLGPPPRGFVLPDLTGRDGSGTARGLRDE
                     GFSVAIRAAGTARGPSDAVTGQDPPPGHRVAPRDSIVLYVNP"
     gene            complement(5242..6606)
                     /gene="rsmB"
                     /locus_tag="VFM00_01585"
     CDS             complement(5242..6606)
                     /gene="rsmB"
                     /locus_tag="VFM00_01585"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET7496967.1"
                     /translation="MTRRPAPPRRRKPVGPRELALQILAAAESRRAYSDRLLESRLRD
                     SRLDPRDAGLVTTLVQGTLRHRALLDHHLAALTGPRWDELPLWIREVLRIGAFQILVL
                     TRVPSSAAVDESVKLAKKYGHPGTAGLVNAVLRRLAGGAVAPLPDAATDPAARLAIQH
                     SHPRWLVDRWVARYGAEEAALLLAADNVEPRVSVRANRARTSTEALAEALRAEGFAVE
                     PGKNGGPVLIVGDGFTAARSPLFREGLLSLQDEAEACVVPVLDPRPGDRALDVAAAPG
                     GKASQIAEAVQPAGSVVALERHPSRARALRANLEERLGLARAGIVCGDGTAPPLRGPF
                     GRVLVDAPCSGLGVLRRRADARWRKEPGAIAEMAALQTALLEASAPLVLAGGVLVYSV
                     CSLEPEETDAIVTPFLSRHPEFSLDDARPFLPPSFRGSEPFFRALPHVHGTDGVFAAR
                     LKRR"
     gene            complement(6603..7646)
                     /gene="fmt"
                     /locus_tag="VFM00_01590"
     CDS             complement(6603..7646)
                     /gene="fmt"
                     /locus_tag="VFM00_01590"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET7496968.1"
                     /translation="MRLVYYGTPALAVPPLRRLVLDGRAPLLVVTRPDRPRGRGLASS
                     ASPVKEAALELGLPVETPPRAGAPEALERVRALQPDLLIVAAYGQIFPRALLAVPRLG
                     ALNVHYSLLPRHRGAAPVQAALLSGDAETGVTTMWMTEGLDEGPVFLSRSTAIAPGED
                     AGALGARLAALGAECLAESLDRIEAGEILREEQDPARATMAGKIRAADARLDPGESPE
                     VLARRVRAFAPEPGAHFVLMSSPAAGAPSPEAGAPAAGGSGDRLLVLAAEPGSATRSE
                     VAPGTLLAIDRARGMVIALPQGSLWLLRVKPAGRKTMSGAEYANGKRLKPGDRLPLAT
                     GAAAPGARSKASP"
     gene            complement(7698..8231)
                     /gene="def"
                     /locus_tag="VFM00_01595"
     CDS             complement(7698..8231)
                     /gene="def"
                     /locus_tag="VFM00_01595"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7496969.1"
                     /translation="MARNLRYYGDPVLRRKAAPAQAGPELAALVADLFDVMYREKGVG
                     LAAPQVGESTRVFVVDVEDEVGRTKQAFVNPVITKREGTMNGEEGCLSIPGYREDVKR
                     WAAIEVQATDENGHPFTLEAEGLLARAIQHELDHLDGILFLDRLSPIKRKLLEARLKR
                     FQAPVHAMDAEGSRSSL"
     gene            complement(8233..8559)
                     /gene="yajC"
                     /locus_tag="VFM00_01600"
     CDS             complement(8233..8559)
                     /gene="yajC"
                     /locus_tag="VFM00_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET7496970.1"
                     /translation="MLDAVYAQAAPVAAPSPADQMLHMVAILAITIGIFYFLIIRPQQ
                     KKQKEAEQMLASIRKGDRVLTSGGVYGTVIGTGAKDDVVVLRVAEDVKMEFSKQAIVQ
                     VVERGS"
     gene            complement(8563..>8790)
                     /locus_tag="VFM00_01605"
     CDS             complement(8563..>8790)
                     /locus_tag="VFM00_01605"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HET7496971.1"
                     /translation="AACARFTRAYLRHLHQAGEILAHRMLTLHNVTFYQTLMKEAREA
                     IDRGAFDVYEREFRRRYRARRDDASPPEAQE"
BASE COUNT         1268 a         3126 c         3184 g         1212 t
ORIGIN      
        1 accagctctt cacgacgatc gggatgcccg cgcccaccgc ccacgaagtc aggttgtcat
       61 cgggataccc gtcgagcgtc gcggacttcg aattgaaccc cactccgaga tcgatcccga
      121 ccttctgtcc ggccagccac cagcgcacgc cgaccggcgc gtcgacgtcg tggaacccca
      181 ggccgatgct cttgtgctcg gccggaggca tgtcgtccgc cagcgccagc gccggcagga
      241 tcgtgagaag cgcgaacaag gtcacggcca gaatcgacag cgatcggttc atgcgtttcc
      301 ctcctgggaa aggtccgtcg gttcgttcgg ccgcggcatg tgcccgatgc cgcggccagg
      361 gtcgcccgtc aggcacggcg tgattccgtt tccggccgga taacgctaga ccaccggcct
      421 ccgtcccgag acgagacgga tgatgatggc gatgaccgca aggacgagca ggatgtggat
      481 gaggccgcca agggtgtagt gggtgagcat gcccagcagc cacatgacca cgagcaggac
      541 ggcgatggtt tcgatcatga cgttctccca atcgtaagga acgcgaacgc gttccgtgag
      601 ccgcgacgtt cgtacgacgg ctgggatggt aggcactcct acgaagcggg tcaatcgagg
      661 gagatggaag gaggttgttc cgaggcggcg tgggtcccct ggagcgcggc gaccgcccag
      721 cgcctgtgaa tcggagaaca agttgtgttc cggcgcgtta cacgcgtcgg gtcatctcgg
      781 catgctgttt gctcaacagc cgcgtcgaag aatgttccgc cacggtcgga aaaatgtcga
      841 cacgtcctcc ggcggcttcc accgtctccc gtcccaccac gtcctcaggc ttgtagtcgc
      901 cgcccttcac gaggacatcc ggctggagcg cctcgatgac gctacgcggc gtctcgtcgt
      961 cgaagacgaa gacgccatcc acgtcggtga gcgcggcgag gatggcggcc cggtcggcct
     1021 cgctctgcac cggacggccg cggcccttca gcgcgtggac cgaacggtcg gagttcaacc
     1081 cgacgagaag gtagtgcccc tggcgcttgg cgtgcgccag cagcgcgacg tggcccggat
     1141 ggaggatgtc gaagcagccg ttcgtgaaga cgaaggtctg ggagaggacc cgcgacgccg
     1201 cccgccggcg aagcgcatcc tcgaacgtga gcacctcacc caagggccgg ctcctccagc
     1261 gaggcgatca gctcggcggc ccccgcggcc gccgttccca catgcgccac gacgagcccc
     1321 gcggcgtgat tggctaggac gcaggcctcg gggagcgtcg cctcggcggc gagggcggcg
     1381 cccatcgcgg cgacgaccgt gtcccccgcg cccgtgacgt cgtagacctc gcgggcgcgc
     1441 gtcgggaagt gcgtgtgccc ctccgcggtg aagagcgaca tcccctcctc gccgcgcgtg
     1501 atcagcacgg agcgcgcgtc cagccgctcg cgcagtccga agccggcctc gaggagcgat
     1561 tcggtgtcgc gcagcttccg cccccaggcc tgcgaggcct cagccacgtt cggcgtgatc
     1621 acggtgaccc ctcgatagcg gaagaaatgc tcttccttgg gatccaccgc caccgggacg
     1681 ccgcggcgcg agagctcggg aagcagccgg tccaaaagct cgggcgtgac cacgcccttg
     1741 ccgtagtcgc tcacgatggc ggcgtcggcg cggccggccg cggcgcggat ccgctcccag
     1801 agcgcttcct gggcggcggc gccggcgggt tcccggctct cttcgtcggc gcggagcacc
     1861 tgctggtggt gggcgatgat gcgggtcttg agcgtggtgc ggcgggcggc gtctttgacc
     1921 acgcccgtct cgggctccac gcgccgctcg gccaggagcc gcgcgcactc ttcgcccgcg
     1981 gcatcggcgc cgagaaggcc gcagaggatg ggcgtcgccc cgagcgcgcg cacgttgtgc
     2041 gcgacgttcg cggcaccgcc cagccggagc tcctcccgtt ccacgtccac gaccagcacc
     2101 ggcgcctcgg gcgagacgcg gctcgcgcgc ccccagagat agcgatccag gatcacgtcc
     2161 cctacgacca ggaccgtgcg cccggccatg gcccgcacgc gcgcggccag ggcgccgccc
     2221 ggccctcgcg agggaacggg aacggccccg ggaggagcgc tgggaagcgg cttcacgcct
     2281 cgtcggctcc ttcgatgtcg accttccgcc gcgtcgtggt cctcggacgc gaggccaggt
     2341 agtcgcggat cagcgccttg gccttcgcct cgtcgcgttc gagcacctcg atcttccccc
     2401 agtacccatg ccccaccgat tccacgccgg ggagcatgga gatctccacg ggctgcagca
     2461 tggcaaggac cccctgctcg cgcaggaaat cggccagcat cgtgccggtg gcttcgtcgg
     2521 gcacgtcgtg cacgggcacc agatcttcgg gtatcttgtc gggctccagc tcgtcttcgt
     2581 cgtgcttccg gcgctcggtc attcctcgta tccctccggg aagcgctgcc gccactgcca
     2641 ggcagtctcc accatttcgt tgagttcctc gaactgccgc gtccagccta acacgcgcca
     2701 cgcctcggcg gcggaggcga tcaggaccgg aggatccccc gcccggcgcg gcgaatcctg
     2761 cgtcgggacg ggcaacccgg tgacctcctc gacggttcgg atcacctgcc gcaccgaact
     2821 cccggtgccg gtgccgaggt tcaaggtcac gctttcgccc ccgtcggcca ggtagcgcat
     2881 cgcgcgcaga tgggcgtcgg cgatatccac gacgtggatg tagtcgcgga tcgccgtgcc
     2941 gtcctcggtc ggatagtcgc tgccgtagac cgggaccggc cccttcgccc cgcgcgccgc
     3001 gtcgatcacg agcgggatca ggtgggtctc gttccggtgg tcctcgcccc ggtcgatgct
     3061 cgcgcccgcc acgttgaagt agcggagggc gacgtagcgg agcccgtcgg tctcgccccg
     3121 ctcgcgcagc atccgctcga agcggagctt ggtctcgccg tagggattcc cgggcttcaa
     3181 cggcgaatcc tcgcgcagcg gcatcggcgc cgccgcgtcg tacaccgcgg ccgtcgatga
     3241 gaagaccatg tgcttcacgc cgtggtcgcg catggcgtcg agcaggatct tcccgtactc
     3301 cacgttcgcc cgctcgtaca ggtcggggtg cgcgaccgat tcggagacga tcgacttggc
     3361 cgcgaggtgg atgacgcagt cgatctcctt ggtcgtgaag gcctcgcgga ggcgctcggg
     3421 atcctggatg gccccggcca ccaggaacgc cagcggcggc accgccgccc ggtgccccgt
     3481 ggagagctcg tcgaggatga tcgtcgagtg gcgcgcgcgc acgagctctt cggccacgat
     3541 gctgccgatg tagccggcgc cgccgacgac cagggcgttc acggccgggc gagggacgcg
     3601 cggagggcgc gaaccgccgc cggcggatcg ggggcgtgaa agatggagtg tcccgcgacg
     3661 aaggcgtcgg cgccggcgcg cccggccagc ggcgccgtat ggacgtcgat cccgccgtcc
     3721 accatgacga ggcagtccag cccgcgctcc gcgacggcgc gccgcgtcgc ggcgatccgc
     3781 tcgggagatc ccgggaggaa cgcctggccg ccggcgccgg gctcgaccgt catgacgagg
     3841 gcgatgtcgc agtcggggag cgctggaatc aggcgctcca tcggcgtggc gggcttgatc
     3901 gagagccccg cgcgcaggcc gcgggcgcga atggcccgcg ccgcggcggc gggatcgtcc
     3961 acggcttcca cgtgcacgat gacatggtcg gcgcccgcgt cggcgaacgc gggaaggtgg
     4021 cggagcggat cgacgatcat gaggtgcacg tcgagcggaa ggcgggtcat cttccggatc
     4081 ccggcgacga cgatgggccc gaacgtgaga ttgggaacga actgcccgtc catgacgtcg
     4141 acgtggatga agtcggcccc cgccgcttcg acgctcgcga tcgcctcggc gaggcgcgcg
     4201 aagtcggcgg agaggatcga ggggcagacg cggcatcgcc cgggagcgga ccacgtcccg
     4261 gcggcgcccc gcgcttccgg ggcccccttc atggattcac gtagaggacg atcgaatcgc
     4321 gcggggcgac ccggtgtccc ggcggcggat cctgtccggt gacggcgtcg gagggtccgc
     4381 gcgccgtccc cgccgcgcgg atcgccacgg agaatccctc gtcgcgaagg ccccgcgcgg
     4441 ttcccgatcc gtcgcgtccc gtcagatcgg ggagcacgaa gccgcgcggc ggcgggccga
     4501 ggctgaccag caggtccacc accgtctcct gctccacgac cgtctcaccc ggaggatcgc
     4561 tcgcgatcac gaggtccttt ccgatctcgt cggtgtagac gcgcgccacc ttgccgcggc
     4621 gaaggcccga gctctcgagc tggatgccgg cctgccgctc ggacacgccg tccagcacgg
     4681 gaacgctggt cccctgcgcg cccaggctca cgacgacggc gatgcgccgc ccgcgcttca
     4741 cctggccgcc cgcgggcggg tcctgcgccg cgatgaagcc gcgcggcacg tcggggctcc
     4801 actgctcgga gatcttggag agccggaggt cctccgaggc cagcacgcgc tcggcctcgc
     4861 gctcggagag tccggtcact tcgggaaccg gcacctcggc gccctgccgc gtgaagctgg
     4921 gcatgagcac caggttgatc accaggaacc cgccgaggag cgcgatcgcc gagaggagga
     4981 gcgtgccggt gatgaggttg aagcgcggac cgcggcggcg ctccgcgcgg ggcggcgccg
     5041 tggcctccgg cgagagggaa tccagcgcga gcggcgaggc gtcgatcgcc cggccgccgt
     5101 cgcccgacgg cggcggctcg gcgagcttcg tttccggtgc ggtgccgagc gagacgcccg
     5161 gctcggcctc gcggacgcgt tcggcttcgc gctgcgcgat cgcgcggcgg cgccagggct
     5221 cgacgggacg gcggcggccc ctcatcggcg cttgagcctc gccgcaaaca cgccgtcggt
     5281 gccgtgcacg tgcggcagcg cgcgaaagaa gggctcggaa ccgcgaaacg aaggcgggag
     5341 gaacgggcga gcgtcgtcga gggagaattc cggatgtcgg ctcagaaacg gcgtcacgat
     5401 cgcgtcggtc tcctccggtt cgagggagca gacactatac accagaacgc cgccggccaa
     5461 gacgagcggc gccgacgcct cgagcagcgc cgtctgcagc gccgccatct ccgcgatggc
     5521 gccgggctcc ttgcgccagc gcgcgtccgc gcggcggcgg agaacgccca ggccggagca
     5581 gggcgcgtcg acgagcacgc ggccgaacgg gccgcgaagc ggcggcgcgg tgccgtcgcc
     5641 gcacacgatc ccggcgcgcg cgaggccgag acgctcttcg aggttcgcgc gaagcgcgcg
     5701 cgcccggctt ggatgccgct ccagcgcgac gaccgagccc gccggctgga cggcctcggc
     5761 gatctgcgag gccttgcccc ccggcgcggc cgcgacgtcg agcgcccgat cgccggggcg
     5821 cgggtcgagg actggcacga cgcacgcctc cgcctcgtcc tgcagcgaca ggaggccttc
     5881 gcggaagagc ggcgagcgcg ccgcggtgaa cccgtccccc acgatcagca ccggccctcc
     5941 gttcttcccc ggctccaccg cgaatccctc ggcgcgcagc gcttccgcga gcgcttcggt
     6001 cgaggtgcgc gcccggttgg cgcgcacgga gacgcgcggc tcgacgttgt cggcggcgag
     6061 gaggagcgcc gcctcttcgg cgccatagcg cgccacccac cgatccacca gccagcgcgg
     6121 atgcgaatgc tggatcgcga ggcgggccgc cgggtcggtc gcggcgtccg gaagcggcgc
     6181 cacggcgccc cccgccagcc gccgcagcac cgcgttcacg agcccggccg ttccgggatg
     6241 cccgtatttc ttggcgagct tcacgctctc gtccacggcc gccgaggaag gcacgcgcgt
     6301 gagcacgagg atctggaacg ccccgatccg aagcacctcg cggatccaga ggggaagctc
     6361 gtcccagcgt gggccggtga gcgcggccag gtgatgatcg agcagcgccc gatggcgcag
     6421 cgtcccctgc accagcgtcg tgaccagccc ggcatcgcgc ggatccagcc tcgaatcgcg
     6481 gaggcggctc tcgagcagcc ggtcggaata ggcgcggcgg ctctccgccg cggccaggat
     6541 ctggagcgcg agctcgcgcg ggccgaccgg cttccggcgg cgcggcggcg cggggcggcg
     6601 cgtcacggcg aagccttcga gcgcgcgccg ggcgcggccg cgccggtggc gagcgggagc
     6661 cgatccccag gcttgagccg ctttccgttg gcgtactcgg cgccggacat cgttttcctt
     6721 cccgccggct tcacacgcag aagccagagc gacccctgtg ggagcgcgat caccatgccg
     6781 cgggcgcggt cgatcgcgag cagggtgccg ggggcgactt cagagcgagt cgccgagccg
     6841 ggctcggcgg cgagcacgag gagccggtca ccggagccgc ccgcggcggg ggcgcccgct
     6901 tccgggctgg gggcgccggc ggccggcgac gacatcagga cgaaatgggc ccccggctcg
     6961 ggggcgaacg cgcggacccg gcgggcgaga acctccggcg actcgccggg atcgagccgc
     7021 gcgtcggcgg cccggatctt cccggccatc gtcgcgcgcg cggggtcctg ttcctcgcga
     7081 aggatctcgc cggcctcgat ccggtcgagc gattcggcca ggcattcggc gccgagcgca
     7141 gcgagccgcg cgccgagcgc tccggcgtcc tcgcccggcg cgatcgccgt gctccgcgag
     7201 agaaagaccg gcccctcgtc gagcccctcg gtcatccaca tcgtggtcac gccggtctcg
     7261 gcgtcgcccg aaaggagcgc ggcctggact ggggcggccc cgcgatgacg cggcaggagc
     7321 gagtagtgca cgttgagggc gcccagccgg ggcacggcga gcagggcccg gggaaagatc
     7381 tgtccgtagg cggcgacgat caggagatcg ggctgaagcg cgcggacccg ctcgagcgcc
     7441 tcgggcgcgc cggccctggg cggcgtctcg accggaagcc cgagctcgag cgcggcctcc
     7501 ttgaccgggg atgccgacga ggcgagaccg cgacccctgg gacggtcggg acgcgtcacc
     7561 acgaggagcg gggcgcgtcc atccaggacg aggcgccgga gcggcggcac ggcgagcgcg
     7621 ggcgtgccgt agtagacgag gcgcatatca gtccctggga gcgggtggag ggcggaggcc
     7681 ggtcggccgc acgccggtca gagcgaggag cgggagccct cggcgtccat cgcatggacg
     7741 ggcgcctgga agcgcttcaa gcgcgcttcg aggagcttcc gcttgatcgg gctcaagcgg
     7801 tcgaggaaga ggattccgtc caggtgatcc agctcgtgct ggatcgcgcg ggcgaggagt
     7861 ccctcggctt cgagcgtgaa ggggtggccg ttctcgtccg tggcctggac ctcgatcgcg
     7921 gcccagcgct tcacgtcctc gcgatacccg gggatgctca ggcatccctc ctcgccgttc
     7981 atggtccctt cgcgcttcgt gatcaccggg ttcacgaacg cctgcttcgt gcgtccgacc
     8041 tcgtcctcga cgtccacgac gaagacgcgc gtcgactccc ccacctgcgg cgccgccagc
     8101 ccgacgccct tctcgcgata catcacgtcg aagagatccg ccacgagcgc ggccagctcg
     8161 ggccccgcct gggcgggcgc ggctttccgg cgcagcaccg gatcgccgta atagcggagg
     8221 ttccgggcca cttcagctcc cacgctccac gacctggacg atcgcctgct tcgagaactc
     8281 catcttcacg tcctccgcca cgcgaagcac gacgacgtcg tccttggcgc cggttccgat
     8341 cacggtgccg tagacgccgc cggaggtgag gacgcggtcc cccttccgga tcgaggccag
     8401 catctgctcg gcttccttct gcttcttctg ctgcgggcgg atgatgagga agtagaagat
     8461 tccgatcgtg atcgccagga tcgcgaccat gtgcagcatc tgatcggcgg gcgacggcgc
     8521 ggcgacgggc gcggcctgcg cgtacacggc atccagcatg gattactcct gggcctcggg
     8581 cggggaggcg tcgtcccggc gcgcgcggta gcggcggcgg aactcgcgct cgtacacgtc
     8641 gaaggcgccg cgatcgatcg cctcgcgcgc ctccttcatg agtgtctggt aaaacgtgac
     8701 gttgtgcaag gtcagcattc ggtgggcgag gatctccccc gcctggtgca ggtggcgcag
     8761 ataggcgcgc gtgaaacgcg cgcacgccgc
//
LOCUS       DASSLZ010000045        16496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1789293, whole genome shotgun sequence.
ACCESSION   DASSLZ010000045 DASSLZ010000000
VERSION     DASSLZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 16496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16496
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789293"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1438)
                     /locus_tag="VFM00_01610"
     CDS             complement(32..1438)
                     /locus_tag="VFM00_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017930834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HET7496972.1"
                     /translation="MNRWTCALAIVSASLLSAPAAHSFCGFYVARGDAKLFNKASKVV
                     LVRDEDHTVLTMASDFKGEPEEFALVVPVPTVLEKGQIHVGDPKAVDHLDAYTAPRLV
                     EYFDPDPCRVYAMEKMSALRNAAPMAAGQAAGDARLDRSLGVRVEARYTVGEYDILIL
                     SAKESGGLETWLRIHDYRVPPGASRVLASYLRQGMKFFVARVNLAEQAKLGYSYLRPL
                     QMAFDSPKFMLPLRLGMVNADGPQELFVYALTRNGRVETTNYRTVRLPSDAEIPEFVK
                     EDFPRFYKDMFAEQHEHAGGDAVFLEYAWDMAWCDPCAADPMSREELAGLGVFWINTG
                     IVPPGRRGGPGIAPQFMPPPGNPQEVFVTRLHVRYDAAHFPEDLVFQETGDKTSFQGR
                     FVIRHPFRGQGECEAMRAYRGQVRERRAKEAETLADLTGWSMRRIHQRMGGDAEWSSA
                     PGPAPGEGRKWWDWMWKK"
     gene            complement(1371..2255)
                     /locus_tag="VFM00_01615"
     CDS             complement(1371..2255)
                     /locus_tag="VFM00_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HET7496973.1"
                     /translation="MTRRFDPRWWQICCLGGLLTYGLLRLHFDTPPVQIAATIGTALA
                     AQLLFTRLFRIPTFEWKSALISGLGLCFLFRANDWRLAVLCASIAIGSKFLVRWNGKH
                     IWNPTNIALVVMMIAFDRAWVSPGQWGSGPTLAYAMAMAGAFVVNRSARSDTTIAFLA
                     AYAAVLFGRSMWVGEPLRIAAHRMTSGALMIFAFHMISDPKTTPNTRAGRILFASLVA
                     AGAGAVAFVLFRPNGPLWALAALAPAVPLIDRLLPGPKYEWKRPVVGPLWKGDLREPM
                     DVRAGDRVGEPALGPSRA"
     gene            2647..3606
                     /locus_tag="VFM00_01620"
     CDS             2647..3606
                     /locus_tag="VFM00_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HET7496974.1"
                     /translation="MRRVPWHIAFAAGAAVALASAAAIATAQSSPPDQTAAAHATYVG
                     MQACQDCHEDQFKSWEHNPHHGSQIDSNPTGKKEVECESCHGPGSLHVEAGGDKDNPG
                     FHTIRNLKTMKADASSEVCATCHRTAEQFHWKSSTHARNRVACVDCHAVHSPKDPGGQ
                     LLLQATGTSELCLRCHTDKRSQVAHVSHMPVPEGGITCADCHNPHGSPGPHMIRGASN
                     NELCESCHMDKRGPFLWEHPPVREDCLNCHEPHGSNNDKMLVTKRPFLCQRCHIATRH
                     PSTLYDLPDLTASNRTRIVDRGCVNCHTQIHGSNHPSGETLLR"
     gene            3616..5790
                     /locus_tag="VFM00_01625"
     CDS             3616..5790
                     /locus_tag="VFM00_01625"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HET7496975.1"
                     /translation="MKRLRQIQWALPAMIVLSIAAACPVFAADGEVTIGSQWYNQTTR
                     EPKWFDEYRDITNGPMIESFVILDKIDGGRYSLTGTNVLKHDQSEALMYRRPRFTVGV
                     SYKEIPHNLSWNTKTPYARIGPGVYVLPDAVQRRIQSDSASTPARTADLQSLLAGART
                     EPMGFRTDVTRALLKGRIGNGLQLDIRGSRRMRQGDKTYSITLGGFSNVVEIPEDINQ
                     QIVTGEAKLSYVKRRCVIEVSGGLEGFINEVDALIVDNPRIASDSTIGSSRGRLDLYP
                     DNRTVRGALRAGLELPHHSVFNAYAGISETKQDDRFLPYTINSALPVFALPSPDAHAK
                     AVTVTQDYRLHSSPSRFLNTTLRFRRSDYENKTAFHTFPAEILYDQSVVVGDIENERF
                     EYARSTSGVDLDLTPVSRLVLGGTAEYIWRTRSIREVTKDKELAFEGHVNYHPSISGL
                     VLEGRYRHGDRRLNHTPIEELADEREQPNLRRFDVGDRNQDWGRARLGFQPNGRSDIS
                     AYYEYIRNKYEDQRLDGIGAIASPTDTIGQLGLLDETRRNVGANVSYELTKQITLRGG
                     VGYSKLYTNQRSRTSPATMTQVADSTWQARIVDRFVFGNAAVEWRPKADRLSFGATWE
                     LERTPTSYDLSSIGLRPRAQDLPSTEYRRMGAGVEGWYQLEKGTTSLGLRWNWEEFHV
                     QDFQASVIPLLYPAVNPTILFMADNFRDYRAHILSVMLRRTF"
     gene            6241..7995
                     /locus_tag="VFM00_01630"
     CDS             6241..7995
                     /locus_tag="VFM00_01630"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HET7496976.1"
                     /translation="MSAATGRPAKTHAPAASDAQWPMAAKNWQNTRFSELNQITPANA
                     GRLRLAWTFSTGVKKGQEAAPIVVGNTMYVVTPYPNILYALDLTKAGTPGVLKWKYVP
                     PTIPAAQGVACCDVVNRGAVYADGKVIINTLDGQTIAVDAASGRELWKTRVGNIHIGE
                     TITMSPLVVRDKVFVGNSGGELGVRGWIKALAVSNGRLLWTAYSTGPDSAVKIGPRFR
                     AFYPSEKGKDLGVHTWPPGQWEIGGGNVWGWVSYDPDLNLIYHGTGNPGVWNPDLRPG
                     DNKWTCGLFARDADTGEARWFYQWTPHDLFDHDGVNENVLVDLPLGRSVRKVLLSAQR
                     DGYLYVLDRASGQVLSATPFVRVTAYKGVDLKSGRIQPNEAKETHTGVVVRDIAPASP
                     GGKDWQPTAWSPRTRLLYIPHQTLAMDYEGTDANYIEGTPYVGADVKMYPDPVRPGDG
                     SRGAFTAWDPVRARPVWEIKEPFPVWSGTVVTAGDVAFYGTMDGWFKAVDARTGRVLW
                     RHKTDSGIIGQPVTYRGPDGKQYVAVLAGVGGWSGAIVSGPIDPRDRGAALGFVNAMT
                     DLPKATPAGDKLYVFALP"
     gene            8048..8656
                     /locus_tag="VFM00_01635"
     CDS             8048..8656
                     /locus_tag="VFM00_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7496977.1"
                     /translation="MLIVIGVTSYARETRDFHPQAFAAAPAAGPRGGALHAGPSPEST
                     QVESAGRPGEYKETAQALYEGQQLFQSMNCAGCHSDGGGGMGADLMDARWFYGHEPRQ
                     IFDTIVQGRQNGMPSFGGRIPAYQVWELVAYVRSLSGLVSPDVSSGRNDHMEVMPPPM
                     SLRMKSRLMVKPLPPVDSAAVKGTTVPGVAIHPDSTGAKGAP"
     gene            8653..9624
                     /gene="coxB"
                     /locus_tag="VFM00_01640"
     CDS             8653..9624
                     /gene="coxB"
                     /locus_tag="VFM00_01640"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7496978.1"
                     /translation="MIRVLPGPQSPLDPAGIQALRIEHVWWLFFGITAVVYVVVVAIM
                     LVAAIRRRNAPEPLRPEEDPERERRLIRIVAAALGVTVVLLLVMLVGDFTARRSLRSL
                     EPGDPLEIQVTGHQWWWEFQYQDPIPSNNVTTATELHLPAGRPVEFVLRSADVIHSFW
                     VPNLAGKKDLFPGRLTRLFVRADRVGAYTGQCAEFCGNQHASMRFQVIVDPPQRFQQW
                     LAAQRAPAAAPVTAEQRRGQEVFLGTTCVMCHTVQGTPARSHVGPDLTHVASRQWIAS
                     GFLPNTRAHLAEWVADPQRIRPGVRMPLHPLPPQDLRALLEYLESLR"
     gene            9621..11591
                     /gene="ctaD"
                     /locus_tag="VFM00_01645"
     CDS             9621..11591
                     /gene="ctaD"
                     /locus_tag="VFM00_01645"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET7496979.1"
                     /translation="MSSARQPMAGRERDRGREIAEIAALERTWDRPRGFWGWVISTDH
                     KEIGLRFIGTAFLFFAFSGVLAALMRAQLARPENTLLGPDRYNQFFTMHGTGMMFFFA
                     VPVMEGMALYLVPLMIGTRNVTFPRLLNFSYWTFAFAGILLFAGLFFNMGPDVGWFSY
                     VPLAGPAFAPGHRVDLWAQMITLVEASSLGGAVEIVTTVFKQRAPGMSLDRIPLFVWS
                     QVVTSVMILFAMPSVQLATSLLGFDRTAHVTTQFFNPAEGGDPLLWQHLFWFFGHPEV
                     YIIFLPANGFISAMLPAFTRRSVFGYPALVLSNIATGFLGFGVWAHHMFATPLPELGQ
                     GLFTAASLMITIPTAINIFCWLATIGTGRVRLRLPMLFILSFFAVFVIGGLTGVMLGS
                     VAINSQVHDTFFVVAHLHYVLIGGAVFPLLGALCFWFPKWTGRMPGERLGIVSFILLF
                     VGFNATFFPMHLLGLMGMTRRVYTYIPETGWGPLNALATVGAGILALGFVLFFVNLAW
                     SRRNGLAAGENPWGSDTLEWATLSPPPRYNFGNPPTVKSRDPLWLDPDDTPVVTGLST
                     EAREVLVTTAHDAMPHHRLHLAGESPWPFVTAFLCAGTQMGMVFRPHAYVIGLPLIGV
                     ALALWFWPSHGPAPLVVPRAAQRESGAPGVTT"
     gene            11601..12311
                     /locus_tag="VFM00_01650"
     CDS             11601..12311
                     /locus_tag="VFM00_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496980.1"
                     /translation="MISKPTLDVSNLPASSLDGETPLWWGNVIAMAVETTILSLLLMS
                     YFYLRKNFHLWPPPYVDTLHPVLHPSPKLLLATLNTLLMVASCWPMARADKLARRFAS
                     LSPQSHPPSERERWERESNEARGRVQRSLTGGLALVAAMIAVSLLLRWFGFRALYFDW
                     NDNAYASAAWSLLVLHFVYLLFSFVEMALLAVWIGALGLQEKMAEDLTLVAGFWYWTV
                     AAWIVIYAVVFWSPRLMP"
     gene            12308..12694
                     /locus_tag="VFM00_01655"
     CDS             12308..12694
                     /locus_tag="VFM00_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496981.1"
                     /translation="MNRSPALYGDGPAPAPGGPERLPSLWFGLLAGPAAWALQLPLVY
                     ALAVWSCDHVGMAALHVVSVLAVAAALAGGFAAWRNLRRLESPADASAGTRRLMALLG
                     LMTAGLFGLVALGSWLAVFLLSPCPA"
     gene            12712..13689
                     /locus_tag="VFM00_01660"
     CDS             12712..13689
                     /locus_tag="VFM00_01660"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET7496982.1"
                     /translation="MAAALLLVIGVAFAARAVPAGAHPEHDAATRAAYGDASPEREAP
                     RSWSDLARAWEFDPGVVIPLLLTGVLYFRGVWGGHGLRRGVRTREVACFAGGWLTLVV
                     ALVSPLHPWGEALFSAHMTQHELLMLVAAPLLVLGSPLAPTLRALPRGWTHALARGGR
                     GRTWTALVRALRNPIVAWAVHGTVLWAWHAPALFDAAVENETVHALQHLSFLGSAVLF
                     WWALLHGRGRAMAYGAAVLYLFTTAMHSGLLGALLTFSETPWYPAYAATAHFWGVSAV
                     EDQQLGGLIMWVPACSLYLVVALALFARWMRLSGDMARRAEERATVGKP"
     gene            13719..14750
                     /locus_tag="VFM00_01665"
     CDS             13719..14750
                     /locus_tag="VFM00_01665"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET7496983.1"
                     /translation="MKGGLARWLLAGALAESALSCGRGEPAHWVCVSNERSGTVTVID
                     GSTRAAIATIRLGKRPRGIHASPDGRTLYVALSGSPIAGPPGSTPVPLPPPDRSADGI
                     GVVDLRALALARTLHAGVDPEQFAVSPDGSTLYVANEDAATLSLVRTADGAVTRVIPM
                     GDEPEGVGVAPGGREVIATCEGFGDVFVLDPTGIPRARLVVSGRPRSVAFLPDGSRAF
                     IPAETNGTLTRVDLHRRAADTTFTLPPGSRPMDTVMAPDGKTLYVTNGRAGTVSVVDA
                     ATLRLRGNVRVGPRPWGAGLSPGGRWLYVANGPSNDVSVVDTRSLKEAARIPAGQGPW
                     GIAVVPAPR"
     gene            14938..16053
                     /locus_tag="VFM00_01670"
     CDS             14938..16053
                     /locus_tag="VFM00_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020279102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET7496984.1"
                     /translation="MTLRSARPVPRITPAAVALLIASIATAAPHPARASQTLSTFSIV
                     ACDTVTQELGVAVQSKYFSVGATVPWAEAGVGAVATQANVNPSLGPQALALLRSGLSA
                     PEVMRALAAADTAWDGRQFGIVDAKGRVANWTGKRCLDWAGGETGVAFACQGNILAGP
                     AVVQGMARAFRETHGELAERLLAALEAAQAAGGDKRGQQSAALIVVRPSATHPEYRER
                     YIDLRVEDHKTPIKELRRVWQIHQGFHLANAHLTYATELEAAGKPELAKLERDRVGET
                     LQRALARGERDPSVLNGLAWACASNSVFLEDARKAAERAVNADPRNVDILDTLGEVYF
                     RLGENAKAVEVEARAERIDPKSQYLKDQIARFKGEGK"
     gene            16125..>16496
                     /locus_tag="VFM00_01675"
     CDS             16125..>16496
                     /locus_tag="VFM00_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET7496985.1"
                     /translation="MAPAPAQATFSIVAYDSTTQELGVAVQSRAFSVGMAVPWAEAGV
                     GAIATQATTNESFGPKGLALLRGTPARPGRPARRLSAAEALRTLLAADSGATHRQVGI
                     VDAKGRSASHTGKDCNPWAGSR"
BASE COUNT         2447 a         5854 c         5638 g         2557 t
ORIGIN      
        1 ggctccggcc gggcgccagg ttccagctag ctcacttctt ccacatccaa tcccaccact
       61 tccggccctc gccgggcgcc ggcccgggcg cggagctcca ctcggcgtcg ccgcccatcc
      121 gctggtggat ccggcgcatg ctccaaccgg tcaggtcggc cagcgtctcc gcttccttcg
      181 cgcgccgctc gcgcacctgc ccgcgatagg cgcgcatcgc ttcgcactcg ccctggcccc
      241 ggaagggatg ccggatcacg aaccgtccct ggaagctcgt cttgtccccg gtctcctgga
      301 agacgaggtc ctcggggaaa tgggcggcgt cgtagcgcac gtggagccgc gtcacgaaca
      361 cttcctgcgg gttgcccggc ggcggcatga actgcggagc gatccccggc ccgccgcgcc
      421 gtccgggcgg cacgatcccg gtgttgatcc agaagacgcc cagcccggcc agctcctcgc
      481 gcgacatcgg atcggccgcg cagggatcgc accacgccat gtcccacgcg tactcgagga
      541 agaccgcgtc gccgcccgcg tgctcgtgct gctcggcgaa catgtccttg tagaagcggg
      601 ggaagtcctc cttcacgaac tccgggatct cggcgtcgga gggaaggcga acggtgcggt
      661 agttggtcgt ctccacccgg ccgttccgcg tgagcgcgta gacgaacagc tcttgaggcc
      721 cgtcggcgtt caccatgccg agacggagcg ggagcatgaa cttgggcgaa tcgaaggcca
      781 tctgcagcgg gcgcaggtac gagtatccga gcttcgcctg ttctgccagg ttcacgcgcg
      841 cgacgaagaa cttcatccct tggcggaggt agctggccag cacgcgcgag gctccgggcg
      901 gaacgcgata gtcgtggatc cgaagccacg tctcgaggcc gccgctctcc ttggccgaaa
      961 ggatcaggat gtcgtactcg ccgaccgtgt agcgggcttc gacgcgcacg ccgagggagc
     1021 ggtcgagccg cgcgtcaccg gcggcctgtc cggccgccat gggcgccgcg ttccggaggg
     1081 cggacatctt ctccatcgcg tacacccggc atgggtcggg atcgaagtac tccaccagcc
     1141 gcggcgccgt gtaggcgtcc aggtggtcca ccgccttggg atcgccgacg tggatctgcc
     1201 ccttctcgag caccgtcggc accggcacca ccagcgcgaa ctcctcgggc tcgcccttga
     1261 agtcgctcgc catcgtgagg acggtgtggt cctcgtcgcg gaccagcacc accttcgagg
     1321 ccttgttgaa cagcttggcg tcgccgcgcg cgacgtagaa gccgcagaag ctatgcgcgg
     1381 ctggggccga gagcaggctc gccgacacga tcgccagcgc gcacgtccat cggttcacga
     1441 aggtctcctt tccagagggg gccgaccacg ggacgcttcc attcgtactt ggggccgggc
     1501 agcaggcggt cgatcagcgg taccgccggg gcgagggcgg ccagggccca gagggggccg
     1561 ttgggccgga agagcacgaa cgcgacggcg ccggcgccgg cggcgacgag cgacgcaaag
     1621 aggatccgcc cggcgcgcgt gttcggcgtc gtcttgggat cggagatcat gtgaaacgcg
     1681 aagatcatga gcgcgccgct ggtcatgcgg tgcgccgcga tccggagcgg ctcgccgacc
     1741 cacatcgagc ggccgaagag cacggcggcg taggcggcga ggaacgcgat ggtcgtgtcg
     1801 cttctggccg agcggttcac gacgaacgcg cccgccatcg ccatggcgta cgccagggtg
     1861 ggtccgctac cccactggcc gggcgagacc caggcccggt cgaaggcgat catcatgacg
     1921 acgagggcga tgttggtggg attccagatg tgcttgccat tccaccgcac caggaacttg
     1981 ctcccgatgg cgatcgaggc gcagagcaca gccaggcgcc agtcgttggc gcggaagagg
     2041 aagcagagcc cgagtccgga gatcaaggcg ctcttccact cgaaggtcgg gatccggaag
     2101 aggcgggtga acaggagctg cgcggccagg gccgttccga tcgtcgccgc gatctgaacc
     2161 ggcggcgtgt cgaagtggag ccggaggagc ccgtaggtca agagcccgcc caggcagcag
     2221 atctgccacc accgggggtc gaagcgccgg gtcatgaagc cgggccgggt cgcgagcgcc
     2281 ggggaagaaa ggctggatcc agccgggatt tcgggtcggg agtgcgctcg gaggagactc
     2341 atcgtacccc cgtttacgcc cgtcgggagc gctccattcc gggcgaaaaa aacgaaccgg
     2401 cccctcgcgc gtggcggggg gccgggggac gattgaatgg aacgatatcc aggacctggc
     2461 ggtcgtcaag ccctggccgg acgaaatgag gcgtcgaagc gaataatttc ttaccaggta
     2521 ccaaggtagc cttgggtttt ccccctgggt ccaataggtt ggcgttgctt gccggacgat
     2581 aaatgcggta acaggaagag cgaaacgttg gcgtcgccgc ggtcgaacca tacctggagg
     2641 ctccgcatga gacgtgtccc ctggcacatc gcgtttgcgg ccggagcggc ggtcgcgctg
     2701 gcatccgcag cggccatcgc gacggcccag tcctccccgc ccgatcagac tgcagcggcg
     2761 catgccacgt acgtcggcat gcaggcctgt caggactgcc acgaagacca gttcaagagc
     2821 tgggagcaca atccgcatca cggctcccag atcgactcga atcccacggg caagaaggaa
     2881 gtggagtgcg agtcgtgcca cggaccgggc tcgctccacg tcgaggccgg cggcgacaag
     2941 gacaaccccg gcttccacac gatccgcaat ctcaagacca tgaaagcgga cgcgtcctcg
     3001 gaggtctgcg cgacctgcca tcggacggcg gagcagttcc actggaagag cagcacgcac
     3061 gcgcggaacc gggtggcctg cgtcgactgc cacgccgttc acagcccgaa ggatccgggc
     3121 ggacagctcc tgctgcaggc gaccggcacc agcgagctct gcctgcgttg ccacaccgac
     3181 aagcggagcc aggtcgcgca cgtgtcgcac atgccggtcc ccgaaggcgg gatcacctgc
     3241 gccgactgcc acaatccgca cggctcgccc gggccgcaca tgatccgggg cgcttcgaac
     3301 aacgagctgt gcgagagctg ccacatggac aagcgcggtc ccttcctctg ggagcacccg
     3361 cccgtccgcg aggactgctt gaactgccac gagccgcacg gctccaacaa cgacaagatg
     3421 ctcgtgacca agcgcccgtt cctctgccag cgctgccaca tcgcgacgcg gcatccgagc
     3481 acgctgtacg acctgccgga tctgaccgct tcgaaccgga cccgcatcgt cgatcgcggg
     3541 tgtgtcaact gccacaccca gatccacggg tccaatcatc cgtccggcga aacgctactg
     3601 agatagggag gggtcatgaa acggcttcgg cagatccagt gggccctccc ggcgatgatc
     3661 gtgctctcga tcgcggcagc gtgcccggtt ttcgcggcgg acggggaagt gacgatcggc
     3721 tcgcagtggt acaaccagac gacccgcgag cccaagtggt tcgacgaata ccgcgacatc
     3781 acgaacggcc cgatgatcga gtcgttcgtc attctcgaca agatcgacgg cggtcgctac
     3841 tcgctgaccg gcacgaacgt gctgaaacac gaccagtcgg aggcgctgat gtaccggcgc
     3901 ccgcgcttca cggtcggcgt gagctataag gagatcccgc acaatctgag ctggaacacg
     3961 aagacgccgt acgcgcggat cggacccggc gtgtacgtgc tgcccgacgc ggtccagcgg
     4021 agaatccagt ccgactcggc gagcacgccg gcgcggacgg ccgatcttca gtccctgctc
     4081 gcgggcgcgc gcacggagcc gatggggttc cgcaccgacg tcacccgggc gctcctgaag
     4141 gggcggatcg ggaatggcct gcagctcgac atccggggca gccggcgcat gcgccagggg
     4201 gacaagacct attcgatcac cctcggtggc ttcagcaacg tcgtcgagat ccccgaggac
     4261 atcaatcagc agatcgtcac cggcgaggcc aagctgtcgt acgtgaagcg ccggtgcgtc
     4321 atcgaggtct cgggcggcct ggagggcttc atcaacgagg tggacgcgct gatcgtcgac
     4381 aacccgcgca tcgccagcga ctccacgatc ggctcttcgc gcggccgtct cgacctctat
     4441 cccgacaacc gcaccgtccg cggggcgctc cgcgccgggc tcgagctgcc gcatcactcg
     4501 gtgttcaacg cgtacgcggg catcagcgag acgaagcagg acgaccggtt ccttccctat
     4561 acgatcaaca gcgcgctccc ggtgttcgcg ctgccgtcgc cggacgcgca cgccaaggcg
     4621 gtcacggtca cgcaggacta ccggctccat tcgtcgccgt cgcgcttcct caacaccacg
     4681 ctccggttcc gtcggagcga ctacgagaac aagacggcgt tccacacgtt cccggccgag
     4741 atcctctacg accagtcggt ggtcgtcggg gacatcgaga acgagcgctt cgagtacgcg
     4801 cgcagcacga gcggagtgga cctcgatctc acgccggtct cccgcctggt cctgggcggc
     4861 acggcggagt acatctggcg cacgcggtcg atccgcgagg tgaccaagga caaggagctg
     4921 gcgttcgaag gacacgtgaa ctatcacccg agcatctcgg ggctggtcct ggagggacgc
     4981 taccggcatg gagatcgccg gctgaaccac actccgatcg aagagctggc ggatgaacgc
     5041 gagcagccga atctccggcg gttcgacgtc ggcgaccgga atcaggattg gggacgggcg
     5101 cggctcggct tccagcccaa tggccggtcc gatatctcgg cctattacga gtacatccgg
     5161 aacaagtacg aagaccagcg cctggacggc atcggcgcga tcgcctcgcc gaccgatacg
     5221 atcgggcagc tgggactgct cgacgagacc cggcgcaacg tcggcgcgaa cgtttcctac
     5281 gagctcacga agcagatcac gctgcgcggc ggcgtgggat actcgaagct ctacaccaac
     5341 cagcgctccc gcacctctcc ggccacgatg acgcaggtcg ccgattccac gtggcaggcg
     5401 cggatcgtgg atcggttcgt gttcgggaac gcggccgtgg agtggcggcc gaaggcggac
     5461 cgtctgtcct ttggagccac ctgggagctg gagcgcacgc cgacgagcta cgatctcagc
     5521 tcgatcgggc tccggcctcg cgcgcaggat ctgccgagca cggagtaccg ccgcatgggg
     5581 gcgggggtcg agggctggta ccagctggag aaggggacga cgtccctggg cctccgctgg
     5641 aactgggaag agttccacgt ccaggacttc caggcgtccg tcatcccgct cctctatccg
     5701 gctgtcaacc cgaccatcct gttcatggcc gacaatttcc gggactatcg cgcccacatc
     5761 ctgagcgtga tgctgcgccg aacgttctaa ccctgaagat ccgcttcggt tgatccgggg
     5821 cgcggcgggc agcaacccgc cgcgcccctt cttcgtaccc ccacgtagcc ctctgcacga
     5881 tccttgcaga accgcgcccg cgaaacgcgg ggctcacccg ccggcgtctg cgcctggcgg
     5941 ttttcgtcgc ctcgaggggc gcggcggagc cgatccggca ggcgggccgg gccccctagt
     6001 tccttgacag tgggatggtt ccggaggagc tccgcattgc tgccgtccga ggtcgttccg
     6061 cgttcccgct ctgcccgccg agaagtgctt cgcccctgga ggcggggacg ttccgctcgc
     6121 gagtccttac gaaatttcaa agagtcgcaa cgcttgccgc gctccgagcg acgccgccgg
     6181 cgcgtccgcg ccgcgaccct ggcggcgctc ggcggcctgc tgctcctgag ctcccacgcc
     6241 gtgagcgccg cgacggggcg tcccgcgaag acccacgcgc ccgccgcgtc cgacgcgcag
     6301 tggccgatgg ccgccaagaa ctggcagaac acgcgcttca gcgagctgaa ccagatcacc
     6361 ccggcgaatg cggggcggct tcggctggcc tggaccttct ccaccggcgt gaagaaggga
     6421 caggaagccg ccccgatcgt ggtggggaac acgatgtacg tcgtgacgcc ctatccgaac
     6481 atcctctacg cgctcgacct gacgaaagcc ggcaccccgg gcgtcctcaa gtggaagtac
     6541 gtgccgccaa cgatcccggc cgcgcagggg gtcgcctgct gcgacgtcgt gaaccgcggc
     6601 gcggtctacg ccgacggcaa ggtcatcatc aacacgctgg acggccagac gatcgcggtg
     6661 gacgcggcga gcgggcgcga gctctggaag acccgcgtcg ggaacatcca catcggcgag
     6721 acgatcacca tgtcgccgct cgtcgtccgc gacaaggtgt tcgtcggcaa cagcggcggc
     6781 gagctgggtg tgcgcggctg gatcaaggcg ctcgcggttt cgaacgggag gctcctctgg
     6841 acggcctaca gcaccggccc cgactccgcg gtcaagatcg gcccacgctt ccgcgcgttc
     6901 tacccctcgg agaaagggaa ggacctgggc gtgcacacgt ggccgcccgg gcagtgggag
     6961 atcggcggcg ggaacgtctg gggatgggtt tcgtacgacc ccgacctcaa cctgatctat
     7021 cacggcaccg gcaatcccgg cgtctggaat cccgaccttc gtcccggcga caacaagtgg
     7081 acgtgcggcc tcttcgcgcg cgacgccgac acgggcgaag cgcgctggtt ctaccagtgg
     7141 actccgcacg atctcttcga ccatgacggc gtgaacgaga acgtgctcgt ggaccttccc
     7201 ctcgggcgct cggtccggaa ggtcctgctg agcgcccagc gcgacggcta tctctacgtg
     7261 ctcgaccggg ccagcggcca ggtgctctcg gcgaccccct tcgtgcgcgt cacggcctac
     7321 aagggcgtgg acttgaagtc gggccgcatc cagccgaacg aggcgaagga aacgcacacc
     7381 ggggtggtcg tgcgcgacat cgcgcccgcc tcgcccggcg gcaaggactg gcagccgacg
     7441 gcctggtcgc cgcgcacgcg gctcctctac atcccgcatc agaccctcgc catggactac
     7501 gaggggaccg acgcgaacta catcgaggga acgccgtacg tcggggccga cgtgaagatg
     7561 tatccggacc cggtgcggcc cggcgacgga agccgcggcg ccttcaccgc ctgggacccg
     7621 gtccgcgccc ggccggtctg ggagatcaag gagccgttcc cggtctggtc cggcacggtg
     7681 gttacggcgg gcgacgtcgc cttctacggc acgatggacg gctggttcaa ggcggtcgac
     7741 gcgcggacgg ggcgggttct gtggaggcac aagaccgatt cgggaatcat cggccagccg
     7801 gtcacctatc ggggtcccga cggaaaacag tacgtcgcgg tgctcgccgg cgtgggcgga
     7861 tggagcggcg cgatcgtgtc cggcccgatc gacccccggg accgcggcgc ggcgctcggg
     7921 ttcgtcaacg cgatgaccga cctgccgaag gccaccccgg caggggacaa gctctatgtc
     7981 ttcgcactcc cctgagcatc gcggcctgcg ccgcctcggc gctgtcgtgc tggttctcgc
     8041 gctagccgtg ctcatcgtca tcggcgtgac gtcgtacgcg cgcgagaccc gcgacttcca
     8101 tcctcaggcc ttcgcggccg cgccggctgc gggtcctcgc ggcggggcgc tccacgcggg
     8161 accttcgccc gagagcacgc aggtcgaatc ggccggccgg ccgggcgagt acaaggagac
     8221 agcgcaggcc ctctacgagg ggcagcagct cttccaatcc atgaactgcg cgggctgcca
     8281 ttccgatggc ggaggcggaa tgggcgccga cctcatggac gcgcgctggt tctacggtca
     8341 cgagccacgc cagatcttcg acaccatcgt gcagggtcgc cagaacggga tgccctcgtt
     8401 cggaggccgg atccccgcct accaggtctg ggagctggtc gcctacgtgc gcagtctgag
     8461 cgggctggtg tcgcccgacg tctcgagcgg caggaacgac cacatggaag tgatgccgcc
     8521 gccgatgtcc ctgcggatga agagccggct gatggtcaag ccgctgccgc cggtcgacag
     8581 cgcggcggtg aagggaacga ccgtcccggg cgtcgcgatc catcccgatt cgaccggtgc
     8641 gaagggggcg ccatgatccg cgtccttccc ggcccgcagt cccccctcga ccccgccggc
     8701 atccaggcgc tccggatcga gcacgtgtgg tggctcttct tcggcatcac cgccgtcgtc
     8761 tacgtcgtcg tcgtcgcgat catgctcgtc gcggcgatcc ggcgccggaa cgcgccggag
     8821 ccgctccgtc cggaagagga ccccgagcgg gagcgccggc tgatccgcat cgtcgcggcc
     8881 gccctcggcg tcacggtcgt gctgctcctg gtcatgctgg tgggcgactt cacggcgcgc
     8941 cgctcgctgc gctcgctcga gcccggcgac ccgctggaga tccaggtgac cggccaccag
     9001 tggtggtggg agttccagta ccaggatccc atccccagca acaacgtgac gaccgcgacc
     9061 gagctccacc tgccggccgg ccgccccgtg gagttcgtgc tccgctccgc cgacgtcatt
     9121 cacagcttct gggtgcccaa cctggccggg aagaaggacc tctttccggg gcgcctgacg
     9181 cggctgttcg tgcgcgccga ccgcgtgggc gcgtacaccg gacagtgcgc ggagttctgc
     9241 ggcaaccagc acgcctcgat gcgcttccag gtgatcgtcg atccgccgca gcgcttccag
     9301 cagtggctgg ccgcgcagcg cgcccccgcc gccgcgcccg tcacggccga gcagcgccgc
     9361 ggccaggagg tgttcctggg gacgacgtgc gtgatgtgcc acaccgtcca gggcacgccc
     9421 gcgaggagtc acgtcggccc cgacctcacg cacgtggcca gccggcagtg gatcgcgtcg
     9481 ggcttccttc cgaacacgcg ggcgcacctg gccgaatggg tcgcggatcc gcagcggatc
     9541 cggccgggcg tgcggatgcc gctgcatccg cttcctcctc aggatctccg cgcgctcctg
     9601 gaatacctgg agagcctccg atgagctccg cccggcagcc gatggccggc cgcgagcggg
     9661 atcgcggccg cgagatcgcc gagatcgccg cgctcgagcg cacgtgggac cggccgcgcg
     9721 gcttctgggg ttgggtgatc tcgaccgatc acaaggagat cgggcttcgc ttcatcggca
     9781 ccgccttcct cttcttcgcc ttctccggcg tcctcgccgc cctgatgcgg gcgcagctgg
     9841 cccgtcccga gaacactctg ctcgggcccg accgctacaa ccagttcttc acgatgcacg
     9901 gcacgggcat gatgttcttc ttcgcggtgc cggtgatgga ggggatggcg ctctacctgg
     9961 tgccgctgat gatcggcacc cggaacgtga cctttccacg tcttctcaac ttctcctact
    10021 ggaccttcgc ctttgccggc atccttctct tcgcgggact cttcttcaac atgggacccg
    10081 acgtcgggtg gttctcctac gttcccctgg ccgggcccgc cttcgctccg gggcaccggg
    10141 tcgatctctg ggcccagatg atcacgctgg tggaggcaag ctcgctcggc ggcgccgtcg
    10201 agatcgtcac gaccgtcttc aagcagcggg cccccggaat gtcgctcgac cggatcccgc
    10261 tcttcgtctg gtcccaggtc gtgacctcgg tcatgatcct cttcgcgatg ccctccgtgc
    10321 agctcgcgac ctcgctcctc ggcttcgacc gcacggcgca cgtgacgacg cagttcttca
    10381 accccgccga agggggcgat ccgctcctct ggcagcacct cttctggttc ttcggccatc
    10441 cggaggtcta catcatcttc ctccccgcca acgggttcat ctcggccatg ctgccggcgt
    10501 tcacccgccg gagcgtcttc ggctacccgg ccctggtgct ctcgaacatc gcgacggggt
    10561 tcctgggatt cggcgtctgg gcgcaccaca tgttcgccac gccgctgccc gagctggggc
    10621 aggggctgtt caccgcggcg agcctcatga tcacgatccc gaccgcgatc aacatcttct
    10681 gctggctggc caccatcggc acggggcgcg tccgcctccg gctgccgatg ctcttcatcc
    10741 tctccttctt cgcggtgttc gtgatcggcg ggctgacggg ggtgatgctc gggtccgtcg
    10801 cgatcaattc ccaagtgcac gacaccttct tcgtcgtggc ccacctgcac tacgtgctga
    10861 tcggcggcgc cgtctttccc ctcctcggcg cgctctgctt ctggttcccg aaatggaccg
    10921 gccggatgcc gggggagcgc ctggggatcg tgagcttcat cctcctcttc gtcggcttca
    10981 acgcgacctt cttcccgatg cacctgctcg ggctgatggg catgacgcgg cgcgtctaca
    11041 cctatatccc cgagaccggg tgggggccgc tgaacgcgct cgccaccgtc ggggcgggga
    11101 tcctggcgct cggcttcgtg ctcttcttcg tcaatctcgc ctggagccgc cggaatgggc
    11161 tggccgccgg ggagaacccc tgggggtcgg acacgctgga gtgggccacg ctgtcgccgc
    11221 ctccgcgcta caacttcgga aatcccccca ccgtgaagag ccgcgacccg ctctggctgg
    11281 atcctgacga caccccggtc gtcaccggcc tcagcaccga ggcgcgcgag gtgctggtca
    11341 cgaccgcgca cgacgcgatg ccgcatcacc ggctccacct ggcgggcgaa tcgccgtggc
    11401 ccttcgtcac cgcgttcctc tgcgccggga cgcagatggg catggtgttc cggccccacg
    11461 cctacgtgat cgggctgccg ctcatcgggg tggcgctcgc gctctggttc tggcccagcc
    11521 acggccccgc gccgctggtc gtccccaggg cggcgcagcg cgagtccggc gcccccggag
    11581 ttacgaccta ggagccgcgc atgatctcca agccgacact cgacgtttcg aatctgcccg
    11641 cctcgtcgct ggacggggag accccgctct ggtggggaaa cgtgatcgcg atggcggtgg
    11701 agacgacgat cctgtcgctc ctcttgatgt cgtacttcta cctgcgaaag aatttccatc
    11761 tctggccccc gccgtacgtg gacacgctgc acccggtgct ccatccgtcg cccaagctcc
    11821 tgctcgcgac gctgaacacg ctgctgatgg tcgcgagctg ctggccgatg gcccgggccg
    11881 acaagctggc gcgccgcttc gcgagcctct cgccacagag ccatcccccg agcgagcggg
    11941 agcgttggga gcgggaatcg aacgaggcgc gcgggcgcgt ccagcggtcg ctgacgggcg
    12001 gcctggcgct ggtcgcggcg atgatcgcgg tttccctcct gctccgctgg ttcggcttcc
    12061 gcgcgctcta tttcgactgg aacgacaacg cgtacgcctc ggccgcctgg tcgctcctgg
    12121 tcctccactt cgtctacctg ctcttctcgt tcgtggagat ggccctgctc gccgtctgga
    12181 tcggcgccct ggggctccag gagaagatgg ccgaggacct caccctggtg gcgggcttct
    12241 ggtactggac ggtcgcggcg tggatcgtga tctacgcggt cgtcttctgg tccccgcggc
    12301 tcatgccatg aaccggtccc cggcgctcta cggagacggt cccgccccgg cgcccggcgg
    12361 acccgagcgg ctgcccagcc tgtggttcgg actcctggcc ggaccggcgg cctgggcgct
    12421 ccagctgccg ctggtctacg cgctcgcggt ctggagctgc gaccacgtcg gcatggcggc
    12481 gctccacgtc gtctccgtgc tcgccgtcgc ggcggcgctg gccggcggct tcgccgcatg
    12541 gcggaacctc cgccggctgg agagtcccgc cgacgccagt gcgggcacgc ggcgcctgat
    12601 ggcgctgctg ggcttgatga cggccggcct cttcggactg gtcgcgctgg gaagctggct
    12661 ggccgtgttc ctcctctcgc cctgccctgc ctgacgatgc ggcgcgtggg catggcggcg
    12721 gcgctcctcc tggtcatcgg cgtggccttc gccgctcgag ccgtcccggc aggcgcccac
    12781 ccggagcacg acgccgcgac gcgggcagcc tacggcgatg cgtcccccga gcgcgaagcc
    12841 ccccggagct ggagcgacct ggcccgcgcg tgggagttcg acccgggggt ggtcatcccg
    12901 ctcctgctca cgggggtgct ctactttcgc ggcgtgtggg gcggacacgg cctgcgccgg
    12961 ggcgtgcgga cgcgcgaggt ggcctgcttc gcgggcggtt ggctcacgct ggtggtcgcg
    13021 ctggtctcgc cgctccatcc ctggggcgag gcgctcttct ccgcccacat gacgcagcac
    13081 gagctcctca tgctggtcgc ggcacccctg ctcgttctcg gaagcccgct cgctcccacg
    13141 ctgcgcgccc tgccgcgcgg ctggacgcac gcgctcgcgc gcggggggag gggccgaacc
    13201 tggaccgcgc tcgtgcgcgc gctccgaaac ccgatcgtgg cctgggcggt gcacggcacg
    13261 gtgctctggg cgtggcacgc ccccgcgctc ttcgacgcgg cggtcgagaa cgaaacggtg
    13321 catgcgctcc agcatctctc cttcctcggc tcggcggttc tcttctggtg ggcgctcctc
    13381 cacgggcgcg ggcgcgccat ggcctacggc gccgcggtcc tctatctctt caccaccgcg
    13441 atgcacagcg gcctcttggg ggcgctcctc acgttctcgg agacaccgtg gtatcccgcc
    13501 tacgcggcca cggcgcattt ctggggcgtg tcggcggtgg aagaccagca gctcggcggg
    13561 ctgatcatgt gggtgcccgc atgctcgctc tatctcgtgg tggccctcgc gctcttcgcg
    13621 cgctggatgc gcctctcggg cgacatggcc cgccgcgccg aggagcgcgc caccgtgggg
    13681 aagccgtgag cggggcctcg agacgcctcg tccggatcgt gaaaggcggc ctcgcgcgat
    13741 ggctgctcgc gggcgcgctc gcggaaagcg ccctctcgtg cggtcgcgga gagcccgcgc
    13801 attgggtctg cgtcagcaac gagcgctccg gcacggtcac ggtgatcgac ggctcgacgc
    13861 gcgcggccat cgccaccatc cggctcggaa aacgcccccg cgggatccac gcgagccccg
    13921 acggacgcac cctgtacgtc gcgctcagcg gatcgcccat cgcgggccct cccggctcca
    13981 caccggtgcc gctgcctccg cccgaccgct ccgcggacgg gatcggggtc gtggatctcc
    14041 gcgcgctcgc tcttgcgcgc acgctccacg ccggcgtcga tccggagcaa ttcgccgtct
    14101 cgcccgacgg ctcgacgctc tacgtggcca acgaggacgc ggccacgctc agcctggtcc
    14161 gcaccgccga cggcgccgtc acgcgcgtga tccccatggg ggacgagccg gaaggggtgg
    14221 gtgtggcgcc ggggggccgc gaggtgatcg ccacctgcga gggctttggc gacgtgttcg
    14281 tcctcgaccc gacgggcatc ccgcgcgcgc ggctggtcgt gtcggggcgg ccccgctccg
    14341 tggcgttcct gcccgacggg tcgcgcgcgt tcattcccgc cgagacgaac ggcacgctga
    14401 cgcgggtgga tctacaccgc cgcgccgccg acaccacctt cacgcttcct ccgggatccc
    14461 gacccatgga caccgtgatg gcgcccgacg ggaagacgct ctacgtcacc aacggccgcg
    14521 ccggcacggt ctcggtcgtg gacgcggcca cgctccggct gcgcggcaac gtgcgggtgg
    14581 gcccgagacc ctggggcgcg ggcctttcgc ccggcggacg ctggctgtac gtggcgaacg
    14641 gcccctccaa cgacgtctcc gtggtggaca cccgctcgct gaaggaagcg gcccgcatcc
    14701 cggcgggtca ggggccctgg gggatcgcgg tggtccccgc ccctcggtag cgtcccaccc
    14761 cgtcccgcgc cccgggcgcg tcacacccag tcccgcgcct cgggagcgtc accgcctcct
    14821 ccaatagccg ccccttcccc ccggcggccc ccgccgtagc cgaggcaccc ccgttttgct
    14881 accttcgggg gactccgtct ccacggctcc gcttccgctt cgaagggggt attcccgatg
    14941 acgcttcgct ccgcgcgccc ggttcctcgg atcactccgg ccgccgtcgc gctcctcatc
    15001 gcgtcgatcg ccaccgcggc gccgcacccg gcccgcgcga gccagaccct gagcaccttc
    15061 tccatcgtcg cgtgcgacac cgtgacgcag gagctcgggg tcgccgtgca gtccaagtac
    15121 ttcagcgtgg gcgccaccgt tccctgggcc gaggcgggcg tcggcgcggt cgcgacgcag
    15181 gcgaacgtga atccttcgct cggtccccaa gcgctggcgc tcctgcgctc cgggctctcc
    15241 gcccccgagg tgatgcgcgc cctggccgcc gccgataccg cctgggacgg ccggcagttc
    15301 gggatcgtgg acgcgaaggg gcgcgtggcc aactggaccg ggaagcgctg cctcgactgg
    15361 gccggaggtg agacgggcgt cgcgttcgcg tgtcagggga acatcctcgc cggccccgcg
    15421 gtggtgcagg ggatggcgcg cgcgtttcgc gagacgcacg gagagctggc cgagcggctc
    15481 ctggcagcgc tggaagcggc gcaggcggcg ggcggcgaca agcgcgggca gcagtcggcc
    15541 gcgctcatcg tcgtgcgtcc cagcgcgacc catcccgagt accgggagcg ctacatcgac
    15601 ctccgcgtcg aggaccacaa gacgcccatc aaggagctgc gccgcgtctg gcagatccac
    15661 caggggttcc acctggcgaa cgcgcacctg acctacgcga ccgagctcga agccgcggga
    15721 aagccggagc tggccaagct ggagcgcgac cgggtgggcg agacgctgca gcgcgcgctg
    15781 gcgcgcgggg agcgcgaccc ctcggtgctg aacggcctgg cctgggcctg cgcgtccaat
    15841 tccgtcttcc tcgaggacgc gcgcaaggcg gccgagcgcg ccgtaaacgc cgatccgcgg
    15901 aacgtggaca tcctggacac gctgggcgag gtctacttcc gcctgggcga gaacgccaag
    15961 gcggtcgagg tggaggcgcg cgccgagcgg atcgatccaa agagccagta cctgaaagac
    16021 cagatcgcgc ggttcaaggg ggaggggaag tgaggcttcg gcgcacgccg atccggcggg
    16081 gaacgctcgc ggccgcggca gcggccggga cgctcctgtt cgccatggcg cccgcgccgg
    16141 cgcaggccac cttctccatc gtcgcgtacg actcgacgac gcaggagttg ggcgtcgcgg
    16201 tccagtcgcg cgccttcagc gtggggatgg cggtgccgtg ggccgaggcg ggcgtcgggg
    16261 ccatcgcgac gcaggccacc accaacgagt cgttcggtcc gaaggggctg gcgctcctgc
    16321 gcggaacgcc cgcacgaccc ggccgcccgg cgcggaggct gtccgccgcc gaggcgctgc
    16381 gaacgctcct tgccgccgac tcgggggcga cccaccggca ggtggggatc gtggacgcga
    16441 aggggcggag cgcctcgcac acggggaagg actgcaaccc gtgggcgggg agccgt
//
LOCUS       DASSLZ010000046         5660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1155321, whole genome shotgun sequence.
ACCESSION   DASSLZ010000046 DASSLZ010000000
VERSION     DASSLZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155321"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="VFM00_01680"
     CDS             complement(<1..1000)
                     /locus_tag="VFM00_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496986.1"
                     /translation="MDCRATARSAAAALLAAFVIVAGTAARVAPAAAQSSADSLASRA
                     PVLRTPAGWETLATGLPEEARLKLRPGFRYDRVDGAVPAMGVAFQSERDPDPFVYVAA
                     SYATGRDRFLGEAGFEAPIGDPTWLRAGADVYRRTATEDAWIVGDLENTIFALFARTD
                     YRDWYEATGWDGHVTLEPGRDLAVTGSVRVQSESSLPTRVRFSLFGKHDRFRPNPPVR
                     SGDEGLVSVSLRMGPERLPTAGGTQGTAAYERAGGPLGRDFDYGRARGSVRTLHRFSK
                     RMLLRARAIGGSTREGALPPQKIWHVGGIGTLRGHDYKAFSGDQFFVANAEYLVKVR"
     gene            complement(975..2327)
                     /locus_tag="VFM00_01685"
     CDS             complement(975..2327)
                     /locus_tag="VFM00_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7496987.1"
                     /translation="MTASRIRVLVVDDEPSVLLETSASLKRSYEVLTASRAEDAEKML
                     ADQRVDLILTDLRLPGKDGIALLESVKASHPDVPVVLMSGHGTIEEAVKAIRLGAVDF
                     VEKPFGPERLQVTVERALELKALQRENERLRSITGAADEMVGKSRVLDQIRGEASKVA
                     KTDAKVLITGESGTGKELVARSIHRMSARARGPFEKLNCAALPKDLVESELFGYEKGA
                     FTGAAQMKRGRLEAADQGTLFLDEVGDMSLETQAKFLRAIETGDIERLGGTRTIAVDT
                     RILAATNKDLPAEIQAGRFREDLYYRLNVVPIHIPPLRARREDVPLLVAHFVQRFGAE
                     HPRGPRTVTPAAMERLTQYSWPGNIRELKNLIERLLIMTDGDAIEIADVEDALPINAG
                     DEPPSEIRAARDKAERDTILAMLRDCQWNVSEASRRLGMDRGYLHRKIKRYGLSRDGA
                     "
     gene            complement(2324..3931)
                     /locus_tag="VFM00_01690"
     CDS             complement(2324..3931)
                     /locus_tag="VFM00_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7496988.1"
                     /translation="MFGTLHRKLLALFFLVALIPSLGLTLFVTQYLAKSLAALRNPET
                     ERALAQSLEVIRQGIERLGSDAQHHATVMSEDDAATRLLAQGTLPEVERWLRDEARER
                     GLDYVVLYRNDAKPARVFGARFGPRITIPEPERESLLAALNEGGLVSGGEVPRQVSGV
                     ATFGKNYLILAGYQLDPEISSEIEALQKNLTMYKRLGVYTWLSERSLWVFAGLWSVVL
                     GLVSFFLAALVSRSIARPIVALESGMERVSTGDLAHRVTPAGTREVRYLGNAFNRMVE
                     EIQTSRRALLRAERLAAWREVARAVAHEIRNPLTPIQFALQRLQEEARRPAPRAEVIG
                     ENAESILREVRSLQEFVTAFSAVAQLPEPKPAPCDVPALVEDVARLYRGSSRVEFRVE
                     VERPVPLAWADSGQIQRVLVNLIKNAIEAMGKEGAVTLRVRRDAGAFPGAASAVALEV
                     EDTGPGMDPATLERAAHPGFTTKSSGSGLGLTLVQRIVEQHGGRFELDSSPGVGTRAT
                     VTIPTVPEGDEAPAAPLASGDGAGARA"
     gene            complement(3943..5013)
                     /gene="rlmN"
                     /locus_tag="VFM00_01695"
     CDS             complement(3943..5013)
                     /gene="rlmN"
                     /locus_tag="VFM00_01695"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET7496989.1"
                     /translation="MRPGRGDARFEDILGLDPEELARVALGLGQPAYRGRQLAEWLYR
                     RGELDLSRMSNLPAAFREQLAAAHRVGSLSEIGRRETPDRKTRKIALRLPDGGIVESV
                     YMSTPTRFTFCLSSQHGCGFACRFCATGRMGRGRNLAAGEIVEQALHLRRELPPGVSE
                     YNVVLMGMGEPLENYDAVLRALALLSDPALGAVPPKRITISTVGLVPQIYRLADEAPR
                     YRLAISLHAATDELRSKLMPVNRTFPLEALMGAVRHHVDRTGRRVTFEYILIEELNDT
                     VAHAAKLVKLVAQLPCKINLIPYNPIGPGKFRRPAAERIERFAAYLQPRVPAVTVRYS
                     QGVEIGAACGQLAGAAEGGARA"
     gene            5165..>5660
                     /locus_tag="VFM00_01700"
     CDS             5165..>5660
                     /locus_tag="VFM00_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496990.1"
                     /translation="MRIGAVDIGTNSVRLLVADVDDEERLRTSHRMGEISRLGEGLDR
                     TGLIDEGAAARTLECLERFVHEAEYSGASTIRVAATNALRVATNGPDVAARFSERIGY
                     PVEILTGEEEARLVFLAVLSGLNPRPRRSVVVDIGGGSTEIIFGEGQEGNQVISLELG
                     CVRLT"
BASE COUNT          829 a         1974 c         2027 g          830 t
ORIGIN      
        1 tgcgcacttt caccaagtac tcggcgttgg ccacgaagaa ctgatcgccc gagaaggcct
       61 tgtagtcgtg gccgcgaagg gtgccgatcc cgcccacgtg ccagatcttc tgaggcggaa
      121 gggcgccctc gcgcgtcgag ccgccgatgg cgcgcgcccg gagcagcatc cgcttcgaga
      181 agcggtgcag cgtgcgcacg gagccgcgcg cgcggccgta atcgaaatcg cggcccagcg
      241 ggccgccggc gcgctcgtag gcggcggtcc cctgcgtgcc gcccgccgtg ggcaggcgct
      301 cgggacccat ccggagcgac accgagacga gcccctcgtc gcccgatcgg accggcggat
      361 tggggcggaa gcgatcgtgc ttgccgaaga gggagaagcg cacgcgggtg ggaagcgagg
      421 actcgctctg cacgcggacc gaccccgtga cggcgaggtc gcgccccggc tccagcgtca
      481 cgtggccgtc ccatccggtc gcttcgtacc agtcgcggta gtcggtgcgc gcgaacagcg
      541 cgaagatcgt gttctccagg tcgcccacga tccaggcgtc ctcggtcgcg gtgcggcggt
      601 agacgtccgc ccccgcgcgg agccacgtag gatcgccgat cggcgcctcg aatcccgcct
      661 cgcccagaaa gcggtcgcgg ccggtcgcgt aggaagcggc tacatagacg aacgggtcgg
      721 gatcgcgctc gctctggaac gcgacgccca tcgcgggcac ggcaccgtcc acacggtcgt
      781 agcggaaccc gggccgcagc ttgagccgcg cctcttcggg cagcccggtg gcgagcgtct
      841 cccatcccgc cggcgttcgc aggacgggcg cgcgcgaggc caggctgtcg gccgaggatt
      901 gcgcggcggc cggcgcgacg cgcgcggccg tacccgcgac gatcacgaac gccgcgagga
      961 gcgccgcggc ggcgctacgc gccgtcgcgc gacaatccat agcgcttgat cttccggtgg
     1021 agatacccgc ggtccatgcc caggcggcgc gaggcttcgc tgacgttcca ctggcagtcg
     1081 cggagcatgg cgaggatcgt gtcgcgttcg gccttgtcgc gcgccgcgcg gatctcgctg
     1141 ggcggctcgt cccccgcgtt gatcggaagc gcgtcctcca cgtcggcgat ttcgatcgcg
     1201 tcgccgtcgg tcatgatcag gaggcgctcg atcaggttct tcagctcgcg gatgttcccg
     1261 ggccacgagt actgggtcag ccgctccatc gccgccgggg tgaccgtgcg cggaccgcgc
     1321 ggatgctcgg cgccgaagcg ctggacgaag tgcgccacca ggagcggcac gtcctcgcgc
     1381 cgcgcgcgga gcggcgggat gtggatcggg accacgttca accggtagta gaggtcctcg
     1441 cggaagcgcc cggcctggat ctccgccggg aggtccttgt tcgtggcggc caggatccgc
     1501 gtgtccacgg cgatcgtgcg cgtcccgccg aggcgttcga tgtcgccggt ctcgatggcg
     1561 cggaggaact tggcctgcgt ctccaggctc atgtcgccga cctcgtccag gaacagcgtc
     1621 ccctggtcgg ccgcctcgag gcggccgcgc ttcatctgcg ccgcccccgt gaaggcgccc
     1681 ttctcgtagc cgaagagctc gctctcgacc aggtccttgg gaagcgccgc gcagttcagc
     1741 ttctcgaacg ggccgcgcgc ccgcgcgctc atccggtgga tcgagcgggc gaccagctct
     1801 tttcccgtgc cgctctcgcc ggtgatgagc accttggcgt cggtcttcgc gaccttggag
     1861 gcttcgccgc ggatctgatc cagcacccgg ctcttcccca ccatctcgtc ggccgcgccg
     1921 gtgatggagc ggagccgctc gttctcgcgc tgcagcgcct tgagctccag ggcgcgctcc
     1981 acggtgacct gcaggcgctc gggcccgaac ggtttctcga cgaagtcgac cgcgccgagg
     2041 cggatcgcct tgaccgcctc ctcgatggtg ccgtggccgg acatgagcac gaccggcacg
     2101 tcggggtggg acgccttcac gctctcgagc agggcgatgc cgtccttgcc gggcaggcga
     2161 aggtcggtga ggatgaggtc cacgcgctga tcggcgagca ttttctccgc gtcctcggcg
     2221 cgcgaggcgg tcagcacctc gtagctgcgc ttcagactgg ctgaggtctc gaggaggacg
     2281 gagggctcgt cgtccaccac gagcacgcga atccgggagg cggtcatgcc cgggcccccg
     2341 ctccgtcgcc gctcgcgagc ggcgcggccg gagcctcgtc cccctccgga acggtgggaa
     2401 tcgtcacggt cgcgcgcgtg ccgacgcccg ggctcgaatc gagctcgaag cggccgccgt
     2461 gctgttccac gatgcgctgc accagggtaa gccccaatcc gctgccgctc gacttggtgg
     2521 tgaagccggg gtgcgccgcg cgctccaggg tggccgggtc catgccgggt ccggtgtcct
     2581 ccacctcgag cgcgacggcc gacgcggcgc cgggaaaggc gccggcgtcc cggcgaacgc
     2641 gaagcgtgac cgcgccctct ttgcccatcg cctcgatcgc gttcttgatc aggttgacga
     2701 gcacgcgctg gatctggccg gagtcggccc aggctaaggg aacgggccgc tccacctcaa
     2761 cgcggaattc cacgcgggag gagccgcgat agagccgcgc cacgtcctcc accagagccg
     2821 gcacgtcgca gggggcgggc ttgggttcgg ggagctgcgc caccgcggag aaggcggtga
     2881 cgaactcctg gagcgagcgc acctcacgca ggatcgactc ggcgttctcg ccgatcacct
     2941 cggcgcgcgg cgcgggacgg cgggcctcct cctggagacg ctgcagcgcg aactggatcg
     3001 gcgtgagggg attccggatc tcgtgggcca ccgcgcgggc cacctcgcgc caggcggcga
     3061 gccgctcggc gcggaggagg gcgcgccgcg aggtctggat ctcctcgacc atccgattga
     3121 aggcgttccc caggtagcgc acctcccgcg tgccggcggg agtgacgcgg tgcgcgagat
     3181 cgcccgtgga gacccgctcc atgccgctct ccagcgccac gatgggccgg gcgatgctgc
     3241 gcgagacgag ggcggcgagg aagaagctga ccaggccgag caccaccgac cagagccccg
     3301 cgaagaccca caggctccgc tcggagagcc aggtgtagac gccgagccgc ttgtacatcg
     3361 tgaggttctt ctggagcgct tcgatctcgg aggagatctc ggggtccagc tggtagccgg
     3421 ccaggatcag atagttcttt ccgaacgtcg ccacgccgct cacctgccgc gggacctcgc
     3481 ctcccgacac cagccccccc tcgttcaacg cggcgagcag ggactcgcgc tcgggctcgg
     3541 ggatggtgat gcggggcccg aaccgcgcgc cgaagacgcg cgccggcttg gcgtcgttcc
     3601 ggtagagcac cacgtagtcc agcccgcgct cccgcgcctc gtcgcggagc catcgctcca
     3661 cctcgggcag cgttccctgc gcgagcagcc gcgtggcggc gtcgtcctcc gacatcacgg
     3721 tcgcgtgatg ctgcgcgtcg ctgcccagcc gttcgattcc ctgccggatc acctcgagcg
     3781 actgggccag cgcgcgctcg gtctcgggat tgcggagcgc ggccaggctc ttggccaggt
     3841 actgcgtgac gaacagcgtg aggcccagcg aggggatgag cgcgacgagg aagaagaggg
     3901 cgagaagctt ccgatgaagg gtgccgaaca tggcggcgcg cgttacgccc gcgcgccccc
     3961 ttccgcggcg cccgcgagct ggccgcaggc cgcgccgatc tccaccccct ggctgtagcg
     4021 caccgtcacc gcgggcacgc gcggctggag ataggcggcg aagcgctcga tccgctcggc
     4081 cgcgggccgc ctgaatttcc cgggaccgat cgggttgtag gggatcaggt tgatcttgca
     4141 gggaagctgc gccaccagct tcaccagctt cgcggcgtgc gcgaccgtgt cgttcagctc
     4201 ctcgatcagg atgtactcga acgtgacgcg ccggccggtg cggtcgacgt ggtgccgcac
     4261 cgcgcccatc agcgcctcga ggggaaacgt ccggttcacc ggcatcagct tggagcgaag
     4321 ctcgtccgtg gcggcgtgca gcgagatggc gagacggtac cgaggagcct cgtcggcgag
     4381 tcggtagatc tgcggcacca ggccgactgt cgagatggtg atccgcttgg gaggcacggc
     4441 accgagggcg gggtccgaca ggagggccag cgcccggagg accgcgtcgt agttctcgag
     4501 cggctcgccc atccccatga gaacgacgtt gtactccgag acgccgggcg gaagctcgcg
     4561 gcggagatgg agcgcctgct cgacgatctc tccggcggcc aggttccggc cgcgccccat
     4621 ccgccccgtg gcgcagaagc ggcaggcaaa gccgcagccg tgctgggagg agaggcagaa
     4681 cgtgaaccgg gtcggcgtgg acatgtagac cgactcgacg atccccccgt ccggaagacg
     4741 gagggcgatc ttgcgcgtct ttcgatcggg ggtctcccgg cgcccgatct ccgacagcga
     4801 tcccacccgg tgggcggccg cgagctgctc ccggaaggcg gcgggaagat tggacatgcg
     4861 ggacaggtcc agctctccgc gccgatacag ccattccgcc agctgccggc cgcgataggc
     4921 gggctggccc agccccaacg ccacgcgagc cagctcctcg gggtcgagcc ccaggatgtc
     4981 ctcgaaccgg gcatcccccc gcccgggccg cacatttcct gttctggtaa gggtttccgt
     5041 ctttacagtc ataggccgct atggtagggt tcgtgcactc ccagagtcaa ccgcgcggag
     5101 gggcaaggcc ggtggaacgc gcctgcttct ggcaaatgtc gccgcagcac cccgactgcg
     5161 accggtgaga atcggcgccg tcgacatcgg aacgaactcg gttcgtctgc tcgtcgccga
     5221 cgtggacgac gaagagcgcc tccgcacgtc ccatcgcatg ggcgagatct cgcggctggg
     5281 cgagggtctt gaccgcaccg gactcatcga cgaaggcgcc gcggcgcgca cgctggagtg
     5341 cctcgagcga ttcgtgcacg aggccgagta tagcggcgcg agcacgatcc gggtcgccgc
     5401 gaccaacgcg ctccgcgtcg cgacgaacgg ccccgacgtg gccgcgcgct tctccgagcg
     5461 catcggctac cccgtggaaa tcctcacggg cgaagaagaa gcgcggctcg tgttcctggc
     5521 cgttctctcc ggattgaatc cgcgcccccg ccgttcggtc gtcgtggaca tcggaggcgg
     5581 cagcacggag atcatcttcg gggaagggca ggaaggcaac caggtcatca gcctggagct
     5641 ggggtgcgtc cggctgaccg
//
LOCUS       DASSLZ010000047         6234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1663345, whole genome shotgun sequence.
ACCESSION   DASSLZ010000047 DASSLZ010000000
VERSION     DASSLZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663345"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFM00_01705"
     CDS             <1..681
                     /locus_tag="VFM00_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET7496991.1"
                     /translation="LLGSGSAEARQAQSAPGGGKPFAERVSEDVGIARFALIRPGLAR
                     GGEPNQGGLHYLRDHGYRTVVSFLTDSAESSWVVNSGMRWIHIPMRSGLFSAQPPTQD
                     QVRQFLSVVTDSTLYPVFIHCHAGKDRTGAMSAIYRMEACGWTKDEALAEMDSFGFSG
                     RYRRLRGYVEAYAGSPSTTATAQATPAGAAPSSSEMTAGMMAPVSKTVEAAPAPASGA
                     QTPPGTSH"
     gene            complement(684..1100)
                     /locus_tag="VFM00_01710"
     CDS             complement(684..1100)
                     /locus_tag="VFM00_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7496992.1"
                     /translation="MSWRAVVAALAVVLAAGCARETGRLTPEMARRVESEGVLHRADN
                     LKFRRSHDVGRYDAGWREGVASIIVTRESVLIHRNEQVFLEINPRSRRFLQVRREGER
                     IRIRSGSGGSSEVWSFEPPDSADAWAKDIRAVIRAR"
     gene            complement(1123..1938)
                     /locus_tag="VFM00_01715"
     CDS             complement(1123..1938)
                     /locus_tag="VFM00_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7496993.1"
                     /translation="MTDLNPHAEQMADESMVRTLDAQARAIWPQESELIRRYGLRDDV
                     RIIDVGCGTGEGSSRLAELYPRAEVLGVDILDVSLNRARTRYAALAPRLRFENQSAFE
                     LREPDRSFDLTVNRHVLHSVPHAERVLAELARVTRRGGRLHLIVEDYGMLHFERDEID
                     PRDFWHEGPARFGAATQTDLFVGRHAYRHLVELGLRDITMEYVIVDTLRVPRETFAQI
                     IEAWRDGYVDVTARYSDMTREQARAYFDQMIANIRDPKGYAVWMVPVMSAIVP"
     gene            2003..2824
                     /locus_tag="VFM00_01720"
     CDS             2003..2824
                     /locus_tag="VFM00_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET7496994.1"
                     /translation="MIVHLIDGTYELFRHFYGLRRFNKGKDAPYGAVLGVLGTVIQMI
                     ETGATHLGVATDHVIESFRNDLYDGYKTGEGIEPALWAQFHPLEEALVAMGVATWPMV
                     ELEADDGIASAARIASEDPRVDKISIWSPDKDLAQCVRGDRIVLVDRRGKKILNEAAV
                     KEKFGVPPSLIPDYLALVGDAADGYPGLPGIGAVGAARLLARYGGIESIPDDALRGAR
                     AQALLFKELATLRTDAPLFRDVEALRWRGATPGFEKLATTMDAARLVERCRKAAP"
     gene            complement(2821..3471)
                     /locus_tag="VFM00_01725"
     CDS             complement(2821..3471)
                     /locus_tag="VFM00_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET7496995.1"
                     /translation="MPHTWDAGVPTPEESLRRLIEGNVRFRASSASAARVWDPKHATE
                     SQRPFAIVLGCSDSRTPVEILFDQGFGDLFVVRVAGNVVAPSVVGSIEFAASQFGSRL
                     VVVMGHTGCGAVTATVHAIETGSGSESRNIRDITDRIAPHIEGLVRAGGEGAAAREAV
                     VREAVRANVRASVDHLRHGSRILEDLVVAERLVVVGAEYDLSSGEVVFFDQARQPG"
     gene            3517..4461
                     /locus_tag="VFM00_01730"
     CDS             3517..4461
                     /locus_tag="VFM00_01730"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET7496996.1"
                     /translation="MPAIATKLRDRYRGALLGLAVGNTLGLPVESWPRERILQKYPNG
                     VREMDALELKLPWDDDLAQAVIIAEAILTHDTLGHDDLAARLLDWYESNGRGMGAQTR
                     AVLNALRHGLLPSEAARMIWERAGNSPAGNGAVMRCAPIAMRWRLDPARLRVETENSS
                     RITHHDPRCGWSALAVNLAIADALNGRTATIESIAAALEAAEASLVLVQSARAASGRD
                     LDELKLDGEAMGYTLKAMQVGLWCLERGHDFEESLVAVVQAGGDTDTNGAVAGAVLGA
                     LHGVGAIPPRWIAGVARAERLAAMADQLLERAEGATSP"
     gene            complement(4448..4933)
                     /locus_tag="VFM00_01735"
     CDS             complement(4448..4933)
                     /locus_tag="VFM00_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7496997.1"
                     /translation="MNEKPVSPAQGRSHSGWWWIGIAIVLVAIALVWMPRRTANPVGQ
                     PPVAQAPATPQAAPGPVVTVAKAAELQTQGAFLVDVRQPEEWAEGHIQGSKLIPLDDL
                     ESRLAEVPRDRNVVVVCHSGNRSKHGRDILLRAGYPQVASMAGGLSAWEAEGRKTVKG
                     M"
     gene            5027..5674
                     /locus_tag="VFM00_01740"
     CDS             5027..5674
                     /locus_tag="VFM00_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7496998.1"
                     /translation="MSHRSKFPALILAVSALALILSAPGLAPAARAQGSDWSITFGSG
                     FAKPEGGAFGAVWKRGTPIVLAMAGQMSTHFELGGEFGFVKFKPDRDSTLIPGVRGGD
                     TDWEMWRIRLRARRFLVSPDAKLAPFIMAGLGIYPISAQSSDSTGTLKVTQTANGVSI
                     GGGADFRAGDAVGFGLEGQYHYIRTNREVLGYKASPVLEVLFVIRWIPGGGAAGS"
     gene            complement(5996..>6234)
                     /locus_tag="VFM00_01745"
     CDS             complement(5996..>6234)
                     /locus_tag="VFM00_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7496999.1"
                     /translation="ARAKPRARRLVIVTTAADDAVNNELCARLGALWRAHGARVESYQ
                     FPAGMGVRHDMIDPEQPYQRTGIVYPALERLIAR"
BASE COUNT          941 a         2186 c         2150 g          957 t
ORIGIN      
        1 ctgcttgggt cggggagcgc ggaggcgcgc caggcccaga gcgctcccgg cggcggaaag
       61 ccgttcgcgg aacgtgtttc cgaggacgtg gggattgccc gcttcgcctt gatccggccc
      121 ggtctggcac gcggcgggga gcctaatcag ggaggcctgc actacctccg cgatcacgga
      181 tatcgcaccg tcgtgagctt cctgaccgac agtgccgaga gctcctgggt ggtgaattcc
      241 ggcatgcgct ggatccacat tccgatgcgc tccggtctct tcagcgccca gcccccgacc
      301 caggaccagg tgcggcagtt cctctccgtc gtcaccgact cgaccctgta cccggtgttc
      361 atccattgcc acgccggcaa ggatcggacg ggcgcgatgt ccgcgatcta tcggatggaa
      421 gcgtgcggct ggaccaagga cgaggcgctg gcggagatgg actcgttcgg attcagcgga
      481 cggtaccggc ggctgcgcgg ctacgtcgag gcctacgccg gctcgccttc gacgacggcg
      541 acggcccagg cgacgccggc cggcgcggca ccttcatcgt ctgaaatgac cgcgggaatg
      601 atggcgcccg tttcgaagac cgtcgaggcg gccccagcac cggcttcggg cgcccagacg
      661 ccgcccggga cctcccacta gcttcaccgc gctcgaatca ccgcgcggat atccttcgcc
      721 cacgcgtccg cgctatcggg cggctcgaag gaccagacct ccgacgatcc cccgcttccc
      781 gagcggattc ggatccgctc cccctccctc cgaacctgca ggaagcgccg gctccgcgga
      841 ttgatctcga gaaagacctg ctcgttccgg tggatcagga ccgattcgcg cgtgacgatg
      901 atcgaggcga cgccctcgcg ccagccggcg tcgtagcggc cgacgtcgtg gctccttcga
      961 aacttgaggt tgtcggcccg gtggaggact ccttccgatt cgacgcggcg cgccatctcg
     1021 ggcgtgagcc ggcccgtctc ccgcgcgcaa cccgccgcga gcacgacggc cagggccgcg
     1081 acgaccgcgc gccaggacat cacgacgacg ggaagcgcgg cctcacggaa cgatcgcgct
     1141 catcacgggc accatccaga ccgcgtagcc cttgggatcc cggatgttcg cgatcatctg
     1201 atcgaaatag gcgcgcgcct gctcgcgcgt catgtcgctg tagcgggcgg tcacgtccac
     1261 gtagccatcc cgccaggcct cgatgatctg cgcgaaggtc tcgcgcggca cgcgcagcgt
     1321 gtcgacgatc acgtactcca tcgtgatgtc gcgcagtccg agctccacga gatggcgata
     1381 ggcgtgacgt ccgacgaaga gatcggtctg ggtcgcggcg ccgaaccggg cagggccctc
     1441 gtgccagaag tcgcgcggat cgatctcgtc gcgctcgaaa tggagcatcc cgtaatcctc
     1501 gacgatcagg tgcaggcggc cgccgcgccg cgtcacgcgc gccagctcgg cgagcacgcg
     1561 ctcggcgtgc ggcacggagt ggagcacgtg gcggttcacg gtgagatcga aggagcggtc
     1621 cggctcgcgg agctcgaagg cgctctggtt ctcgaagcgg agccgcggcg cgagcgcggc
     1681 gtagcgcgtg cgggcccggt tcaggctgac gtccaggatg tccacgccga gcacctcggc
     1741 gcgcgggtag agctccgcta agcgagacga cccctcgccg gtgccgcagc cgacatcgat
     1801 gatgcggacg tcgtcgcgca ggccgtagcg gcggatcagc tccgattcct gcggccagat
     1861 ggcgcgcgcc tgcgcgtcca gcgtgcgcac catcgattcg tcggccatct gctcggcatg
     1921 cgggttgagg tcggtcatgg gcggagagga tacaatcacc cgcactgggg agccaacttc
     1981 ggaagaagaa gggaaaggac cgatgatcgt ccatctgatc gacggaacgt acgagctgtt
     2041 ccgccacttc tacgggctcc gccgtttcaa caaggggaag gacgcgccct acggcgcggt
     2101 ccttggggtt ctcggaaccg tgatccagat gatcgagacc ggcgcgacgc atctgggcgt
     2161 cgcgaccgat cacgtgatcg aatcgttccg caacgatctc tatgacggct acaagacggg
     2221 cgaggggatc gagcccgcgc tctgggcgca gtttcatccc ctggaggaag cgctcgtcgc
     2281 catgggggtc gcgacctggc cgatggtgga gctggaagcg gatgacggca tcgcgtcggc
     2341 cgcgcgcatc gcttcggaag acccgcgcgt cgataagatc tcgatctggt cgcccgacaa
     2401 ggatctcgcc cagtgcgtac ggggcgaccg gatcgtgctg gtggatcggc ggggcaagaa
     2461 gatcctgaac gaggccgccg taaaggagaa gttcggcgtg cctccgtcgc tgatccccga
     2521 ctacctcgcg ctcgtcggcg acgccgccga cggatacccg gggctccccg gcatcggcgc
     2581 cgtcggagcg gcacgccttc tcgcccgata cggcggcatc gagtcgatcc cggacgacgc
     2641 cctccgcggg gcgcgcgccc aggcgctcct cttcaaggag ctggcgacgc tgcgcacgga
     2701 cgcccctctc ttccgggatg tcgaagcact gcgctggcgg ggtgcgacgc ccggtttcga
     2761 gaagctcgcg acgacgatgg atgccgcgcg gctggtggag cggtgccgga aggcggcacc
     2821 ctagccgggc tggcgggctt gatcgaagaa gacgacctcg ccgctcgaga ggtcgtactc
     2881 ggcccccacc acgaccaggc gctcggcgac caccaggtcc tcgagaatgc ggctgccgtg
     2941 ccgcaggtgg tccacggagg cccgcacgtt cgcgcgcacc gcctcgcgca cgaccgcctc
     3001 gcgcgcggcc gcgccctctc ctccggcgcg gaccagcccc tcgatgtgcg gcgcgatccg
     3061 gtccgtgatg tcgcggatgt tgcgcgattc ggatcccgat cccgtctcga tcgcgtgcac
     3121 ggtcgcggtg acggcgccgc agcccgtgtg ccccatcacg accacgaggc gcgatccgaa
     3181 ctgggatgcc gcgaactcga tcgaccccac caccgagggc gcgacgacgt tccccgcgac
     3241 ccgcacgacg aagaggtccc cgaaaccctg gtcgaagagg atctcgacgg gcgtccgcga
     3301 atcggagcag ccgagcacga tcgcgaaggg gcgctggctt tcggtcgcgt gcttcggatc
     3361 ccacacgcgg gccgcgctgg cgctcgaggc gcggaagcga acgttcccct cgatgagccg
     3421 ccggagcgac tcctccggcg tgggcacgcc ggcatcccag gtgtgcggca tgcgcgcact
     3481 ctagccgatc gcgcgccgcg gcgtatactt gccgccatgc ccgccatcgc cacgaagctc
     3541 cgcgaccgct atcgcggcgc cctgctcggg cttgcggtcg gcaacacgct ggggttgccg
     3601 gtcgaatcct ggccgcgcga gcggatcctc cagaagtacc ccaacggcgt gcgcgagatg
     3661 gacgccctgg agctgaagct cccctgggat gacgacctcg cgcaggcggt gatcatcgcc
     3721 gaggccatcc tcacccacga cacgctgggc catgacgatc tcgcggcgcg cctgctcgac
     3781 tggtacgagt cgaacggccg ggggatgggc gcccagacgc gggccgtgct gaacgcgctc
     3841 cgccacgggc tcctcccctc cgaggccgcg cgcatgatct gggagcgggc agggaattcg
     3901 cccgccggca acggggcggt gatgcggtgc gcgccgatcg cgatgcgctg gcggctcgat
     3961 cccgcgaggc tccgcgtcga gaccgagaac agctcccgga tcacccacca cgacccgcgg
     4021 tgcggatggt cggcgctcgc ggtcaacctg gcgattgccg atgccttgaa cggccggacg
     4081 gccacgatcg agtcgatcgc ggcggcgctg gaggcggcgg aggcgagtct cgtcctggtg
     4141 cagtccgccc gggccgcttc gggccgcgat ctcgacgagc tgaagctgga cggcgaggcg
     4201 atgggctaca cgttgaaggc gatgcaggtg gggctgtggt gcctggagcg cgggcacgac
     4261 ttcgaagaat cgctggtcgc ggtggtccag gccggcggcg acaccgacac gaacggcgcc
     4321 gtcgccggcg ccgttctggg cgcgctgcac ggcgtgggcg cgattccacc ccggtggatc
     4381 gcgggggtcg cgcgcgcgga gcggctggcc gcgatggccg atcagctcct ggaacgggcc
     4441 gagggcgcta catccccttg accgtcttcc gtccctccgc ctcccacgcc gacaatcctc
     4501 ccgccatgct cgcgacctgg ggataacccg cccggagcag gatgtcgcgc ccgtgcttgc
     4561 tccgattccc ggagtggcag accaccacga cgttccggtc gcggggcacc tccgccaggc
     4621 gcgattcgag atcgtccagc ggaatcagct tcgacccctg gatgtgcccc tcggcccact
     4681 cctcgggctg gcgcacgtcg accaggaagg ccccctgagt ctgcagctcg gccgctttcg
     4741 cgacggtgac caccggcccc ggcgcggctt gcggcgtcgc gggcgcctgc gcgaccggcg
     4801 gctggcccac cgggttcgcg gtccggcgcg gcatccatac caatgcgatc gcgaccagga
     4861 cgatcgcgat cccgatccac caccaccctg aatggctccg gccctgggcc ggcgacaccg
     4921 gtttctcgtt catgctcggc acccccatcg ttccggggac ggtcccgtcc ccgccttacc
     4981 gtggcgcttg cggtggggca tgccgccatg tatagaatgg gggtctatgt cccaccgctc
     5041 gaagtttccc gctctgatcc ttgccgtctc cgcgctcgct ctgatccttt ccgcacccgg
     5101 actcgcccct gccgcgcggg cgcagggctc ggactggtcg atcacgttcg ggagcggctt
     5161 cgccaagccg gagggcggcg cgttcggtgc ggtctggaaa cgcgggacgc ccatcgtgct
     5221 cgccatggcc ggtcagatgt cgacgcactt cgaactgggc ggcgagttcg gcttcgtcaa
     5281 gttcaagccc gaccgggaca gcaccctgat ccccggagtc cgaggcggcg acaccgattg
     5341 ggagatgtgg cgcatccgcc tccgcgcccg ccgcttcctc gtgtctcccg acgcgaagct
     5401 cgctcccttc atcatggcgg ggctcggcat ctatccgatc tccgcgcagt cctcggactc
     5461 gaccggaacg ctgaaggtca cgcagaccgc caacggcgtg agcatcggcg gcggcgcgga
     5521 cttccgggcc ggcgacgccg tgggcttcgg tctcgagggc cagtaccact acatccgcac
     5581 caaccgcgag gttctcggat acaaggcctc ccccgtgctc gaggtcctct tcgtcatccg
     5641 ctggattccc ggcggaggcg ccgccggaag ctaggagcag ggccccacac tcacgcctca
     5701 ccgagcggaa tggcgatccg gtggaagcag acccctccca cgtagggtcg cgccagcctc
     5761 gaatacgcct cgaaggtcat cctcgcgatc tcgtgatggt ttcccgcctg agcttctcct
     5821 ttcctggcac cggttcggta ctggagcgcg tgaagcgtgc cagggatgag gactgcggcg
     5881 cggttgctct aaggccggtt cgaggcgcgg cttggaagag cgtcgcgttg gatggggacg
     5941 acggggggcc gccgggctcc tgacacattg gcagactcgg aagtactgct ggcagctacc
     6001 gggcgatgag ccgctcgagg gcggggtaca cgatcccggt gcgctgatag ggctgctccg
     6061 gatcgatcat gtcgtgccgg acgcccattc cggcgggaaa ctgatagctc tcgacccggg
     6121 cgccatgcgc gcgccagagc gcgcccagcc tggcgcacag ctcgttgttc acggcgtcgt
     6181 ccgccgccgt cgtcacgatc acgagccggc gcgctcgagg tttcgcgcgg gcgg
//
LOCUS       DASSLZ010000048         8473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1284000, whole genome shotgun sequence.
ACCESSION   DASSLZ010000048 DASSLZ010000000
VERSION     DASSLZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284000"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /gene="rlmD"
                     /locus_tag="VFM00_01750"
     CDS             complement(<1..1061)
                     /gene="rlmD"
                     /locus_tag="VFM00_01750"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET7497000.1"
                     /translation="MTHGEGSQVTPEVEPIQAQEPPDSGPRTGVAAAIRRGDRLELEV
                     TGLAFGGAALAKVDGFVVFVENALPGDRVAATVFRRKRQYAEARAEAILRPSPARVAP
                     RCEHVPICGGCRFQDLDYEEQIRHKERQVAECLAHLGGLTTVVRPAIPAPERFHYRNK
                     MEFSFGKDTSGALTLGLHRRGFFDRSFDLGRCHIARPIASEIVAETRAWARAEGLEPY
                     DTRRHTGLLRFLAVREGIRTGQVMVNLVASAPHSSFAGFAERITARFPSVASVVLNLT
                     RRKAQIAVGEEERVLAGRDRIEERLGDLVFEISSASFFQTNTEQGERLLETALEGLEL
                     RGGERVLDVYSGTGTFTLP"
     gene            1137..1817
                     /locus_tag="VFM00_01755"
     CDS             1137..1817
                     /locus_tag="VFM00_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497001.1"
                     /translation="MPILSRHSAAAALILVLGSAPPGRAATIEGAVTLTPADLSFRGG
                     TRPSAYIGQISGSMARMGAADPSDTTYGVAYLADLSDTAGVSTGPALLDQKGQRFIPR
                     ILAVVAGQAVDFRNSDNVFHNVFSYSPPKKFDLGRYPRGKSKRLTFQKPGLVQVFCDI
                     HSDMRADIVVAPSRRFGYVDAQGRFRIEDVPEGKHTVVVWLPRRGERSGDVEVPASGT
                     AALAIGAE"
     gene            1834..3759
                     /locus_tag="VFM00_01760"
     CDS             1834..3759
                     /locus_tag="VFM00_01760"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7497002.1"
                     /translation="MTQESVTSGTPRTRVSLLFKIAVLTSLPILGIMMAALLLVNHRL
                     AGEVNRNVAADLQRAALAFENQMEREGENLERVGVVVARDPKFFALLTLPRGDRKDAD
                     FHATLLGVAQDFQRDAEAPLFDVTDENGTLLARAGRANAGGAAPDLSDDRSRWPLVRE
                     ALAGRAARGYLVEGERAYRVAVVPIVVDGALTGTLTLGRTIDAALAERLKATTRSEVV
                     FVVNGTASIRTTLRSSLAERLAALPPSPGVVQMADGEHRFLALAGRLEGPTAGGRVGF
                     ALLRSLDQETAVLGRVGRELTIAGVALIVVALLVGILVSTGVTRPVRRIVEAANAMRA
                     GNYDLPLDIRSADEMGTLAAHFEAMREAQRHEIERLEEIDRMKSDFIAIASHEILTPV
                     TTIRAFADLMGEGAAANDAELQREGFRAIRDAAGSLTRLARDLTNMSLIDRKRLPLRL
                     ASADLGLVVEEVAVRTIALAAERRQAVSLSIEPELIHPRVDADYLKQALTNIAQNAVR
                     FTRDGGSIEIGGRRNGGAIELYVADSGIGISKEDFERIFAKIVELKDVNLHSSGTTEF
                     NSSGLGLGLSIARGIVEAHGGEIRVESELGRGSTFTIVIPGEGPARQGGDTSSGPDSS
                     DGSGLSEPGATDLPLAS"
     gene            4569..7115
                     /gene="lon"
                     /locus_tag="VFM00_01765"
     CDS             4569..7115
                     /gene="lon"
                     /locus_tag="VFM00_01765"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET7497003.1"
                     /translation="MLQCDVPSEIPVLPLMSTIVFPLGVTSIQVRIEQSKALLRDHSD
                     PDTLVALVYSPAKREEDIHSGELSRVGLSARIIRILNLPGGNVQVTLEGLRRVAVEEI
                     LRTEPYMVARVTCPQEPVRDPGQVHNLIQRILKALRTLSQVDSGYPAELDNIFSMNLG
                     DPGLFADTVASIVRFPLETKKRVIEALDVQDRLSLVAEAIDSEIARLSVAEDVVRRTS
                     AQMEKTQREFFLRQQLMEIRRLLGEDDPQEMLVRQILDRVDSLALPSHVRSVVQEEAS
                     RLRLLPPTAQESGVIRNYVEWVLSLPWTRDKPHDIRLEDVQRRLNEEHFGLEKVKERI
                     LEYLSVLKLKNDMHGPILCFAGPPGTGKTSLGASIARAMGRSFVRMSVGGVRDEAEIR
                     GHRRTYVGSRPGKILRSLRDARSSSPVFMIDEIDKLGHDSDGDPASALLEVLDPEQNQ
                     HFVDHFIEIPFDLSDVLFITTANVLDFIPGALLDRLEVITIPGYMEEEKLEIARRHLV
                     PRESKEHGLAATDIAFDDAALVKIIREYTREAGVRQLERAIDTFCRKAARQRTTGYTG
                     DWRFSMADVEPILGPPYIVPDVAEGHAEVGVATGLAWTATGGDLLLIEALKMRGAGRV
                     IVTGQLGDVMKESVQAAHSYVRARAELLGINPQLFDDYDLHIHFPEGAVPKDGPSAGI
                     TIVMAIASALAEAPIRNDVAMTGEVTLRGKVLAVGGLKEKVMAAYRAGIKTVVFPSDN
                     QKDLVEIPESIRSKLTFVAVSTVDEVFQHAFAGVAERIAQLEARRKAREAGKAKKDRA
                     AKEKAARRSNGKAPRRARLPKAAGATQDGKPARGKSGRNGPATRPPHRSR"
     gene            complement(7128..7442)
                     /locus_tag="VFM00_01770"
     CDS             complement(7128..7442)
                     /locus_tag="VFM00_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497004.1"
                     /translation="MAPRVAMLVISAVLGLAAAVAAPRSWGVAPFHGILLGAAIYIAA
                     LRHGRDAALRAAAFLALAAAQAWVVFVAAPGADVRATFVILSTWIVLESVGGRPAEAR
                     AR"
     gene            complement(7447..8181)
                     /locus_tag="VFM00_01775"
     CDS             complement(7447..8181)
                     /locus_tag="VFM00_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497005.1"
                     /translation="MRRVHLEVSRGLLAGVAAVLPLLLLASACSNKGERRIAAPDLPS
                     DAPQVSGARWVWEPAALAGAVQAAGKNPLVQRALSQAPIAGLKARFDLAVRAVGEVEG
                     MGPVGLTILPFAVNGDPTRAVFVSVAEGNEQEAAEFAEMIMGREPNADEIGYHSVAWG
                     NQIAWVRSGDAWVVPSGGAHQAPVRRQWTKLFDCLAQRMPTGCAAGATIAEELVPGSP
                     HAAAIGCGIGAAAGGVSCVADFLRDK"
BASE COUNT         1386 a         2917 c         2914 g         1256 t
ORIGIN      
        1 atcggcagcg tgaaggtgcc cgtccccgaa tagacgtcga gcacccgctc gccgccgcgg
       61 agctccagcc cctcgagcgc ggtttcgaga aggcgctccc cctgttccgt attcgtctgg
      121 aagaaggacg cggaggagat ctcgaagacg agatccccca ggcgctcctc gatgcgatcc
      181 cgcccggcca gcacgcgctc ctcctcgccc accgcgatct gcgctttccg gcgggtcagg
      241 ttcagcacga cgctggcgac cgacgggaag cgggcggtga tccgctcggc gaaacccgcg
      301 aaggaggagt gcggcgccga cgcgaccagg ttcaccatga cctgcccggt gcggatcccc
      361 tcgcgcacgg cgaggaagcg gaggagtccc gtgtgtcggc gcgtgtcata gggctcgagc
      421 ccctcggccc gcgcccacgc gcgcgtctcg gcgacgatct cgctcgcgat gggacgggcg
      481 atgtggcagc ggcccaggtc gaagctcctg tcgaagaatc cgcggcggtg gagccccagc
      541 gtgagcgcgc ccgaggtgtc tttgccgaag gagaactcca ttttgttccg gtagtggaag
      601 cgctccggcg cggggatcgc gggacgcacg acagtcgtga ggccgcccag gtgggccagg
      661 cattccgcga cctggcgttc cttgtggcgg atctgctctt cgtagtccag atcctggaag
      721 cggcaccccc cgcagatcgg cacatgctcg cagcgcggcg cgacgcgtgc gggagagggg
      781 cggaggatgg cctcggcccg cgcctcggcg tattggcgct tgcggcggaa gacggtcgcg
      841 gccacccggt cgccaggcag cgcgttctcg acgaagacga cgaacccgtc caccttcgcg
      901 agcgccgcac cgccgaaggc gagtccggtc acctcgagct cgagccggtc cccccgccga
      961 atggccgcgg cgacgccggt tcgcgggccg gaatcgggcg gttcctgggc ctggatgggt
     1021 tcgacttcgg gggtcacctg ggagccctcg ccgtgggtca cgctgagaag tttacaccaa
     1081 gctcgaagcc caagaacggg cttagggtag cccgttcgag ggccgatagg agacgcatgc
     1141 caatcctctc ccgccacagt gccgccgcgg ctctgatcct ggtcctcgga tcggctcctc
     1201 ccgggcgcgc ggccacgatc gaaggcgcgg tcaccctgac gcccgccgac ctctcctttc
     1261 gcggcgggac caggccgagc gcctacatcg ggcagatcag cggaagcatg gcgcggatgg
     1321 gggcggccga tccgtccgac acgacctacg gcgtcgccta tctcgccgac ctctccgata
     1381 ccgccggggt ctccaccggc ccggcgctcc tggatcagaa ggggcagcgg ttcattccgc
     1441 ggatcctcgc ggtcgtcgcc gggcaggcgg tggattttcg gaacagcgac aatgtcttcc
     1501 acaacgtctt ctcctattcc ccgcccaaga agttcgacct ggggcggtat ccgcgcggca
     1561 agtcgaagcg gctgacgttc cagaagccgg ggctggtgca ggtcttctgc gacatccatt
     1621 ccgacatgcg cgccgacatc gtcgtcgcgc cgagccgccg gttcggctac gtggacgcgc
     1681 aagggcggtt ccgcatcgag gacgtgccgg aggggaagca caccgtcgtc gtctggctgc
     1741 cgcggcgcgg tgagcggagc ggcgatgtcg aggtgcccgc gagcgggacc gccgcgctgg
     1801 cgatcggcgc cgagtgagcg gagcgtctcc ggcatgaccc aggagtccgt cacgagcggg
     1861 accccgcgga cgcgggtctc gctcctcttc aagatcgcgg tcctcacctc gcttccgatt
     1921 ctcgggatca tgatggccgc tctcctcctc gtgaatcacc gcctggccgg agaggtgaac
     1981 cggaacgtgg ccgccgacct ccagcgcgcc gcgctcgctt tcgagaacca gatggagcgg
     2041 gaaggggaga acctcgagcg ggtcggcgtg gtcgtggcgc gtgatcccaa gttcttcgcg
     2101 ctcctcacgc ttccgcgcgg agaccgtaag gacgccgact tccacgcgac gctgctcggc
     2161 gtggcgcagg acttccagcg cgacgccgag gcgcctctct ttgacgtgac cgacgagaat
     2221 gggacgctgc tcgcgagggc gggccgggcg aacgccggtg gcgcggcgcc cgacctgagc
     2281 gacgaccgct cccgctggcc tctggtgcgc gaagcgctcg cgggacgcgc cgcgcgggga
     2341 tacctggtgg aaggggagcg cgcctatcgt gtggccgtgg tccccatcgt cgtggatggc
     2401 gcgctcaccg gcacgctgac cctggggcgg acgatcgacg ccgcgctggc ggagcgcctg
     2461 aaggcgacca cgcggagcga ggtggtcttc gtcgtgaacg gcaccgcctc gatccgcacg
     2521 acgctccgga gctccctggc agagaggctg gccgcgctgc cgcccagccc cggcgtggtt
     2581 cagatggcgg acggtgagca tcgtttcctc gctctggccg gccgcctcga ggggccgacc
     2641 gccggcgggc gggtgggctt cgccctgctc cggtccctgg accaggagac cgcggtcctg
     2701 ggccgcgtcg gccgcgagct cacgatcgcg ggcgtggcgc tcatcgtggt cgccctcttg
     2761 gtcggcattc tcgtctcgac cggcgtgacg cggcccgtcc ggcggatcgt cgaggccgcg
     2821 aatgccatgc gcgcagggaa ctacgacctt cctctcgaca tccggtcggc ggatgaaatg
     2881 ggaaccctgg ccgcgcactt cgaggcgatg cgcgaggcgc agcgccatga gatcgagcgc
     2941 ctggaggaga tcgaccgcat gaagtccgac ttcatcgcga tcgcctcgca cgagatcctg
     3001 accccggtca ccacgatccg cgcgttcgcc gacctgatgg gcgagggggc cgcggcgaac
     3061 gacgcggagc tgcagcggga gggattccgg gcgatccgcg acgcggcggg atcgctgacc
     3121 cgcctggcgc gcgacctcac gaacatgtcg ctgatcgacc gcaagcgcct tccgctacgg
     3181 ctggcttcgg ccgacctggg actggtggtc gaggaggtcg cggtgcggac gatcgcgctg
     3241 gccgcggagc ggcgccaggc cgtctctctg tcgatcgagc ccgagctgat ccatccccgc
     3301 gtggacgcgg actacctgaa gcaggcgctc accaacatcg cccagaacgc ggtgcggttc
     3361 acgcgcgacg gcggctccat cgagatcggc gggcgccgga acggaggggc catcgagctc
     3421 tacgtcgcag actcgggcat cgggatctcg aaggaggact tcgagcggat cttcgcgaag
     3481 atcgtcgagc tgaaggacgt caacctgcac tcctcgggga cgacggagtt caactcctcc
     3541 ggcctcggcc tggggctctc catcgcccgc ggcatcgtgg aagcccacgg cggcgagatc
     3601 cgcgtggaga gcgagctggg gcgcggaagc acattcacca tagtgatccc gggtgaaggg
     3661 ccggcccgcc agggcgggga cacttcgagc ggacccgatt cgagcgacgg cagcggtctt
     3721 tcggagcccg gcgccaccga tttgcctctg gcgagctaat aaccccgctt ccgctgggct
     3781 ccgaaaggtc aaccagacga gacatatgtc gtaaaatata gactacacgg ctaaccaatt
     3841 gctccgctgc ggtatacccg ccgcatcccc gaaatgtcaa acgaaggcga catttgaacg
     3901 cctccgcagc cgatcgcgct cgcgttttgc atggtccgca ttttgctgtc ctgtaacggc
     3961 ttactgctac cttccgcgat gccgtgcaaa tggcacgccg gctgctcaag caagtctccg
     4021 tcgaggcact cgcactcagc gagcgacgca atccaaaccc aaaccaacgt ggggtgtgag
     4081 atggaagctc ttcttctgat cgcgatcgtg gtttcgatgt tcggtgacgg cggcggcaag
     4141 gccggctggt aagcaccccg atccaagcag gctgtccctg atccaccgca gcgcaccgtt
     4201 cacgggagag gaggtgaacc aatatggcga cggcaactct gatcctgctc gcgctcttcg
     4261 cgatccacgc gctcgataac tcgggcggag gcaaggcagg ttggtaagcg gtagcgccgc
     4321 acgatagcgg cgccgtccgc agggaagggc gaacccctgt gggcgcttga gcggaccgat
     4381 gggccatcgg aagaagcccc cctggtggcc cgtcgtttcg cgttcggtgg cggaatggaa
     4441 tccagggggg cggctggcca gcggcggggg cgtatgatac cgtggccggt cgtctcccag
     4501 ccaccgaaca cgcaccgcga gaaggggcgc ccccgttgag caaacgtacc gcagccgaaa
     4561 tcgggctgat gctgcaatgc gatgtcccct cggaaattcc ggtgctcccc ctcatgagca
     4621 ccatcgtttt cccactcggc gttacctcca tccaggttcg gatcgagcag tcgaaggctc
     4681 tcctccggga ccactccgac cccgacacgc tggtggccct ggtctacagc ccggccaagc
     4741 gtgaagagga catccattcc ggagagctga gccgggtagg gctctcggcg cggatcatcc
     4801 ggatcctcaa cctccccggc ggcaacgtgc aggtcacgct ggaggggctg cgccgcgtcg
     4861 ccgtcgagga aatcctgcga accgagccct acatggtcgc gcgcgtcacc tgcccccagg
     4921 agccggtgcg ggaccccggg caggtgcaca acctgatcca gcgcatcttg aaggcgctcc
     4981 ggacactgtc ccaggtggac tccggctacc cggccgagct ggacaacatc ttcagcatga
     5041 acctgggaga ccccgggctc ttcgccgaca ccgtcgcctc gatcgttcgc tttccgctgg
     5101 agaccaagaa gcgggtgatc gaggcgctcg acgtccagga ccggctctcc ctggtcgccg
     5161 aggcgatcga ctccgagatc gcgcgcctga gcgtggccga ggacgtcgtc cggcgcacca
     5221 gcgcgcagat ggagaagacc cagcgcgagt tcttcctccg ccagcagctg atggagatcc
     5281 gccggctcct gggcgaggac gatccccagg agatgctggt ccgccagatc ctggaccggg
     5341 tggacagtct ggccctcccg tcccacgtgc ggtcggtggt ccaggaggag gcgagccgtc
     5401 tccgccttct gcctcccacc gctcaggagt cgggggtcat ccggaattac gtcgagtggg
     5461 tcctgtccct cccctggacg cgcgacaagc cgcacgacat ccggctggaa gacgtgcagc
     5521 gccggctgaa cgaggagcac ttcggcctgg aaaaggtgaa ggagcggatc ctcgagtacc
     5581 tgtcggtcct gaagctgaag aacgacatgc acggccccat cctctgcttc gcgggccccc
     5641 cgggcaccgg caagacctca ctcggcgcct cgatcgcgcg cgcgatgggc cgctcgttcg
     5701 tacggatgag cgtggggggc gtgcgcgacg aggccgagat ccgcggccac cgccgcacct
     5761 atgtcgggtc gcgtccgggc aagatcctgc ggtcgctccg cgacgcacgc tcctccagcc
     5821 ccgtcttcat gatcgacgag atcgacaagc tggggcacga ctccgacggc gatcccgcgt
     5881 cggcgctgct ggaggtgctg gatccggaac agaaccagca cttcgtggat cacttcatcg
     5941 agatcccctt cgacctctcc gacgtcctct tcatcacgac ggccaacgtg ctcgacttca
     6001 tccccggcgc gctcctcgac cgcctggaag tgatcaccat ccccggctac atggaggagg
     6061 agaagctcga gatcgcccgc cgccacctgg tgccccggga gtcgaaggag cacggcctgg
     6121 ccgccaccga catcgccttc gacgacgcgg cgctggtcaa gatcatccgc gagtacacgc
     6181 gcgaggcggg cgtgcggcag ctggagcgcg ccatcgacac cttctgccgg aaggccgcgc
     6241 ggcagcggac caccggctac acgggcgact ggcgcttctc catggccgac gtggagccga
     6301 tcctgggccc cccctacatc gtgcccgacg tggcggaggg gcacgccgag gtgggcgtgg
     6361 ccaccggcct cgcctggacg gcgaccggtg gagaccttct cctcatcgaa gcgctcaaga
     6421 tgcgcggcgc cgggcgcgtc atcgtgaccg gacagctcgg cgacgtgatg aaggagtcgg
     6481 tgcaggccgc gcattcctac gttcgcgccc gcgcggagct gctcgggatc aacccgcagc
     6541 tgttcgacga ctacgacctc cacatccatt tcccggaggg ggcggttcct aaagacggtc
     6601 cctcggccgg gatcacgatc gtgatggcga tcgcctccgc gctggccgag gcgccgatcc
     6661 ggaacgacgt ggccatgacg ggggaggtga cgctgcgcgg gaaagtgctc gccgtgggcg
     6721 gcctcaagga gaaggtcatg gccgcctatc gcgcggggat caagacggtg gtctttccct
     6781 cggacaacca gaaggacctg gtcgagattc ccgagtcgat ccgatccaag ctgacgttcg
     6841 tcgccgtgag caccgtggac gaggtgttcc agcacgcctt cgccggtgtg gccgagcgga
     6901 tcgcgcagct cgaggcgcgg cgcaaggcac gcgaggcggg caaggcgaag aaggaccggg
     6961 cggcgaagga gaaggcggcg cggcggagca acggcaaggc tccgcgccgc gcgcgcctcc
     7021 ccaaagctgc gggcgccacg caggacggca agccggcgcg cggtaagagc ggccggaacg
     7081 gaccggcgac gcgcccgcct catcgttccc ggtagccgcc cgcgttttca ccgcgcccgc
     7141 gcctccgcgg gccgccctcc caccgattcg agcacgatcc aggtcgagag aatgacgaag
     7201 gttgcccgaa cgtccgcgcc gggcgccgcc acgaagacca cccaggcctg cgccgccgcg
     7261 agcgccagga acgccgcggc acggagcgcc gcatcccttc catgcctcag ggccgcgatg
     7321 tagatcgcgg caccgaggag aatgccgtgg aagggcgcga caccccagga gcgcggcgcg
     7381 gcgacggcgg cggccagccc caggaccgct gaaatcacga gcatcgcgac acggggagcc
     7441 atggggtcac ttgtcccgga ggaagtcggc gacgcacgag acgccgccgg ccgccgcgcc
     7501 gatgccgcat ccgatcgcgg ccgcgtgggg cgaaccgggc accagctcct cggcgatggt
     7561 ggccccggcc gcgcagccgg tgggcatccg ctgggcgagg cagtcgaaca gcttggtcca
     7621 ttggcggcgc accggcgcct ggtgcgcgcc tccggaagga acgacccagg cgtcccccga
     7681 ccgcacccag gcgatctgat tcccccacgc cacggagtgg tatccgatct cgtcggcgtt
     7741 gggctcgcgg cccatgatca tttccgcgaa ctcggccgct tcctgctcgt tcccctcggc
     7801 caccgaaacg aacacggcgc gcgtggggtc gccgttcacg gcgaagggaa gaatggtcag
     7861 tccgaccggc cccatcccct cgacttcacc gaccgcgcgc accgcgagat cgaatcgagc
     7921 cttgagcccg gcgatcggcg cctggctcag cgcgcgctgg accagcggat tcttccccgc
     7981 cgcctgcacg gcgccggcga gtgccgcggg ctcccagacc catcgcgccc cgctcacctg
     8041 cggcgcgtcg gacggaagat ccggcgccgc gatccgccgc tcccccttgt tcgagcacgc
     8101 gctcgcgaga aggagaagcg gcagtaccgc ggccactccc gcgagcagcc cgcgagagac
     8161 ttccaggtga acccttctca ttacggcacc cctttcctgt cgcgcggcca tgcgcgcgga
     8221 gtggcgccga agagtgaaga ggagatgagt gcgcgagagc aggaagcgtt ccagggggat
     8281 ggggctgcga cttgctcaaa cccttgtcgc gaagagatcg attaaaagag ggaaggcgtc
     8341 cggggtgatt gtggggcgtc ggagtaccga cagtgacaga actacagctc gcgaagcgcc
     8401 tttagaaatt cggcctcggt gacccgctcc ccgtggtctc gtgaggcgac gtacgtgccc
     8461 atataggtgc tca
//
LOCUS       DASSLZ010000049         5379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1664355, whole genome shotgun sequence.
ACCESSION   DASSLZ010000049 DASSLZ010000000
VERSION     DASSLZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664355"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2690
                     /locus_tag="VFM00_01780"
     CDS             <1..2690
                     /locus_tag="VFM00_01780"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET7497006.1"
                     /translation="WVDVPGGRLMGRARLASDQTLDAHVVASIEDLSAMNGLIAPVAA
                     QSGRGRLTADLSGSIRQPNFEARAWLGDGRLTNGAAYDSLQVSAAGRLGSDGTAMAYL
                     TARGIRAAGRPLGNAAATVSFGKKIVIERYVQSAGDSTLAFRGTVTPGKDETTAVLDS
                     MVLTAGTLRFRNLEPVRLLFGQGHVKSASLRLDMRPGRVDVAFDWDLRRARIDADGRL
                     SGIEATRIPGLAASRDSLRGEIEGTFHVSGPLSDPALTVDAAVLRPSWGGIAGDSLAV
                     ALAYAPGVLRIDRVRWAAADGRASLTGTIRAPVSLEAWLRGIAKRDESWAKNAVLDLD
                     ARVDSLDLSLLAPADTSLRSLDGTATFTAHVSGTGLMPVAAIHAASPRIAYRGILGAI
                     TGADLDYAERSLKITRLDLTQSGSVSSITGQIPVDLSFFAKERLLRDRPFQLTVRITD
                     ADFKLASVIYPAYVASSAGKFSVMAGVSGTPRAPAIQGSVKLKDGIVRLAGREEVVNK
                     IQLEGAFDQNQLTITSMTGAQGAKGKLTGSGTWQWGGLGGKAIPIEAGPPGKYAFKVN
                     ASNFTVTDRETYAMQLTGTFTIANGRTEEGKQIPFITGSSTLAKGNLTLDLAATDQEE
                     IVLPFYYDVSVDVPQGLFYRTVDSEVELQGNLRLLNKGEGNLALGTMTVRKGRYYLFT
                     REIQNLSGDFIFSSLDRTDPELAVDGETTIPVSKGSPTVIKVSLTGRASRPEVHLWDP
                     NPDSRYSQADLWRMLTYGQFTPSQEAGTGDGTGGNGGAGGLALPIQAYLFRNAERWLS
                     QSGWIDTFDLGTGARAGGETGAGPLEVGVVGAGKYVTRDLYINYSREFSGAVEQRIGA
                     EYRVTRHLLLKGERTDRNATPGGRPPEEYNLDLKVRLEY"
     gene            2698..4848
                     /locus_tag="VFM00_01785"
     CDS             2698..4848
                     /locus_tag="VFM00_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7497007.1"
                     /translation="MQLNPVQRQAVEHPGGPLLILAGAGSGKTRVLTGRIAHLVQERG
                     VLPEQILAFTFTNKAAREMRSRAAALLGGDDLRVWLGTFHGTCVRILRRHAETLGYPR
                     SFVIYDTDDQRSLLRELLREVGADDKRLTPAGAGSRISGLKNEGIPAAEYAKTAFAPV
                     DRKLAEVYERYERALRDRAAMDFDDLLLYTVKLLDLDEGVHRHYASRFHHVLVDEYQD
                     TNAIQFELIERLSRLHKNLTVVGDDDQSIYGWRGADVGNILSFEQRFPDAAVLRLTQN
                     YRSTQTILRAANGVVKHNTARKEKELWTENAVGDPLTLHVLPDEEAEGEKVVSMLLQE
                     RRDSDRGNKDFVVLYRTNAQSRSVENALRRSAIPYQITGGISFYERREVKDVLAYLRA
                     LVQPKDAVSWYRVLNVPPRGIGRTTVERLQAFAEERKLTVPEALIHPELSAALGVPAA
                     KKLAGVAALFLGMRPFLERSAPACVAEVVAAVRYREYLAEESPAEAEERIENVEELIA
                     GAEAYARRAEDPSVEGFLSEVALLTDVDLWDDTEDAVNLMTVHSAKGLEFPVVFVVGL
                     EEGLLPHASALDEPAELEEERRLFYVALTRARERVHLFHASYRRTWNASGGGISRFVS
                     EIPEDCLLVQEERHWDVEAPRYSPRRSAGAAMRRSEGSLKSAVGLRVIHPQFGEGVVV
                     GSEGFGERAKLTIQFRRAGIKKILAAFAELSHAD"
     gene            4838..5125
                     /gene="gatC"
                     /locus_tag="VFM00_01790"
     CDS             4838..5125
                     /gene="gatC"
                     /locus_tag="VFM00_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET7497008.1"
                     /translation="MPIDAKTVEKIAALARLELQPAEEERMVRELQAILAYVEQLQAV
                     DVGGIEPTSQVTEGKPPALRPDEERPCDVRDEALAQAPDRDGDYFRVPQVV"
     gene            5122..>5379
                     /locus_tag="VFM00_01795"
     CDS             5122..>5379
                     /locus_tag="VFM00_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET7497009.1"
                     /translation="MNESVKGIGATAEAIAAAVRSGSASAEEITRAFLQHADQTAGKL
                     GTYLHRMPEEALRAARAVDAKRASGAPLGALAGVPVAIKDNI"
BASE COUNT          840 a         1854 c         1921 g          764 t
ORIGIN      
        1 gctgggtgga cgtgccgggc gggcgcctca tggggcgcgc gcgcctcgcg tcggaccaga
       61 cgctcgacgc gcacgtggtc gcctcgatcg aggatctgtc cgcgatgaac gggctgatcg
      121 cgccggtggc cgctcagtcg ggacgggggc gtttgacggc cgatctctcg ggctcgatcc
      181 ggcagcccaa tttcgaagcg cgcgcctggc tcggggacgg acgtctcacg aacggcgcgg
      241 cctacgactc gcttcaggtg agcgccgcgg gccggctggg gtccgacggc acggccatgg
      301 cctacctcac ggcgcgcggc atccgcgccg ccgggcgccc cctgggcaac gcggccgcga
      361 ccgtctcgtt cggcaagaag atcgtgatcg aacgctacgt gcagtcggcg ggcgattcga
      421 ccctggcgtt ccgcggcacg gtgacgccgg ggaaggacga aaccaccgcc gttctcgatt
      481 cgatggtgct gaccgcgggg acgctccgct tccgaaatct cgagccggtg cgcctcctgt
      541 tcggccaggg gcacgtgaag agcgcgtccc tccggctcga catgcgcccg ggcagggtcg
      601 acgtcgcctt cgactgggac ctgcgccgcg cgcggatcga cgcggacggc cggctctcgg
      661 ggatcgaggc gacgcgcatt cccgggctcg ccgcgtcgcg cgattcgctt cgcggggaga
      721 tcgagggaac gttccacgtc tcgggcccgc tctccgaccc cgcgctcacc gtcgacgccg
      781 cggtgctccg tccctcctgg ggaggcatcg ccggcgacag cctggccgtc gcgctcgcct
      841 acgcgccggg ggtgctgcgg atcgaccgcg tgcgctgggc ggccgccgac gggcgcgcct
      901 ccctcaccgg cacgatccgg gcgccggtct cgctggaggc ctggcttcgc ggcatcgcca
      961 agcgcgacga gtcgtgggcg aagaacgcgg tgctcgacct ggacgcccgg gtggactcgc
     1021 tggatctctc gctgctcgcg ccggcggaca cgtcgctccg ttcgctggac ggcacggcga
     1081 ccttcacggc ccacgtctcg ggaacggggc tcatgccggt ggcggccatc cacgccgcct
     1141 cgccgcgcat cgcgtatcgc gggatcctgg gggccatcac gggggccgac ctcgactacg
     1201 ccgagcgctc gctcaagatc acgcgtctcg acctgaccca gagcgggagc gtctcgtcca
     1261 tcacggggca gatcccggtg gatctctcct tcttcgccaa ggaacgcctc ctccgcgacc
     1321 ggccgttcca gctcaccgtg cggatcaccg acgccgactt caagctggcc tcggtgatct
     1381 acccggcgta cgtggcctcg agcgccggga agttcagcgt catggcgggc gtctcgggca
     1441 cgccgcgggc gccggcgatc caggggtcgg tcaagctcaa ggacggcatc gtgcgcctgg
     1501 ccggccgcga ggaggtcgtg aacaagatcc agctcgaagg cgcgttcgac cagaaccagc
     1561 tgacgatcac gtcgatgacg ggagcgcagg gagcgaaggg gaagctgacc gggagcggga
     1621 cgtggcaatg gggcggtctc ggcggcaagg cgatcccgat cgaggcgggg ccgccgggga
     1681 agtacgcgtt caaggtgaac gcctccaact tcaccgtgac cgatcgcgag acctacgcga
     1741 tgcagctcac ggggaccttc accatcgcca acggcaggac cgaggaagga aagcagatcc
     1801 cgttcatcac cggatcctcc acgctcgcga aggggaacct cacgctcgac ctggccgcga
     1861 ccgaccagga ggagatcgtg ctgccgttct actacgacgt gagcgtggac gtgccgcagg
     1921 ggctcttcta ccggaccgtg gacagcgagg tggagctgca gggcaatctc cggctgctca
     1981 acaaagggga ggggaacctg gccctcggca cgatgaccgt gcggaagggg cgctactacc
     2041 tgttcacgcg cgagatccag aacctgagcg gcgatttcat cttcagcagt ctcgaccgga
     2101 ccgatccgga gctggcggtg gacggcgaga cgacgatccc ggtctccaag gggtcgccca
     2161 cggtcatcaa ggtttcgctg acgggccggg cgagccgccc cgaggtgcat ctctgggatc
     2221 cgaatcccga cagccgctac tcccaggcgg atctctggcg gatgctcacg tacggacagt
     2281 tcaccccctc gcaggaggcg gggaccggag acggcaccgg cggaaacggc ggcgcgggcg
     2341 gtctcgcgct gccgatccag gcctaccttt tccgcaacgc cgagcggtgg ctgtcgcaga
     2401 gcggctggat cgacaccttc gatctcggca ccggcgcccg cgcgggaggc gagaccggcg
     2461 cgggtccgct cgaggtgggc gtggtgggag cgggaaagta tgtgacgcgc gacctctaca
     2521 tcaattattc tcgcgaattc tcgggggccg tcgagcagcg gatcggcgcg gagtaccgcg
     2581 tcacgcggca tctcctgctc aagggagagc gcaccgatcg gaacgccacg cccgggggcc
     2641 gtcctcccga ggaatacaac ctcgacctca aggtccgact ggagtattaa gcctgacgtg
     2701 caactgaatc cggtgcagcg ccaggccgtc gaacaccccg gcggcccgct cctgattttg
     2761 gcgggcgcgg gcagcggcaa gacgcgcgtg ctcacgggac ggatcgcgca cctcgtgcag
     2821 gagcgcggcg ttctgccgga gcagatcctc gcgttcacgt tcacgaacaa ggcggcgcgc
     2881 gagatgcgct cccgcgcggc ggcactcctc gggggcgacg atctccgggt ctggctgggc
     2941 accttccacg gaacctgcgt gcgcatcctg cgccgccacg ccgagacgct cggctacccg
     3001 cgctcgttcg tcatctacga caccgacgat cagcggagtc tccttcggga gctcctgcgc
     3061 gaggtgggcg ccgacgacaa acggctcacc cccgcgggcg cgggctcgcg catttcgggt
     3121 ctcaagaacg aggggatccc agccgcggaa tacgcgaaga ccgcgttcgc cccggtggac
     3181 cggaagctgg cggaggtcta cgagcgctac gagcgggcgc tgcgcgaccg cgccgcgatg
     3241 gacttcgacg acctcctcct ctacacggtg aagctgctcg acctggacga gggggtgcac
     3301 cgtcactacg cttcgcgctt ccaccacgtg ctggtggacg agtatcagga tacgaacgcg
     3361 attcagttcg agctgatcga acgcctctcg cgcctccaca agaacctgac cgtggtcggc
     3421 gacgacgacc agtcgatcta cggctggcgc ggcgcggacg tggggaacat cctctccttc
     3481 gagcagcgct tcccggacgc ggccgtgctc cggctgaccc aaaactaccg ctccacccag
     3541 accatcctcc gcgccgcgaa cggggtcgtg aagcacaaca cggcgcgcaa ggagaaggag
     3601 ctctggacgg agaacgcggt gggcgacccg ctcaccctgc acgtgctccc cgacgaggaa
     3661 gccgaggggg agaaggtcgt ttcgatgctg ctccaggagc gccgggactc ggaccgcggg
     3721 aacaaggact tcgtcgtgct ctaccggacc aacgcgcagt cgcggtcggt ggagaacgcg
     3781 ctgcgccgct cggccatccc ctaccagatc accggcggga tctcgttcta cgagcggcgc
     3841 gaggtgaagg acgtgctcgc ctatctccgc gcgctggtgc agcccaagga cgcggtgtcc
     3901 tggtaccggg tgctgaacgt gccgccgcgc ggcatcgggc ggaccacggt ggagcgcctg
     3961 caggcgttcg ccgaggagcg gaagctcacc gtgcccgagg cgctgatcca ccccgagctc
     4021 tcggccgcgc tcggcgtgcc cgcggcgaag aagctggccg gcgtcgccgc gctgttcctt
     4081 gggatgcgtc ccttcctcga gcgctcggcg cccgcgtgcg tggccgaggt ggtggccgcc
     4141 gtacggtacc gggaatatct cgcggaggaa tcgccggccg aggccgagga gcggatcgag
     4201 aacgtcgagg agctgatcgc gggcgccgag gcgtacgcgc ggcgcgcgga ggacccctcg
     4261 gtcgagggct tcctctccga ggtggcgctc ctcaccgacg tggacctgtg ggacgacacc
     4321 gaggacgccg tgaacctcat gaccgtgcac tcggccaagg ggctcgagtt cccggtcgtg
     4381 ttcgtggtgg gcctggagga ggggctcctg ccgcacgcct ccgcgctgga cgagccggcc
     4441 gagctggagg aggagcggcg gctcttctac gtcgccctca cgcgcgcgcg ggagcgcgtg
     4501 catctcttcc acgcctccta ccggcgcacc tggaacgctt cggggggcgg gatctcgcgc
     4561 ttcgtctcgg agattccgga ggactgcctg ctggtccagg aggagcgcca ctgggacgtg
     4621 gaggcgccgc gctattcgcc gcgccggagc gccggagccg ccatgcgccg ctccgagggc
     4681 tcgctcaaga gcgcggtggg tctccgggtc atccacccgc aattcgggga gggcgtcgtg
     4741 gtcggaagcg agggattcgg agagcgcgcc aagctgacga ttcaattccg gcgcgcgggg
     4801 atcaagaaga tcctcgccgc cttcgcggag ctgtcccatg ccgattgacg ccaagactgt
     4861 cgagaagatc gccgcgctcg cccgcctcga gctccagccc gccgaagagg agcgcatggt
     4921 gcgcgagctc caggcgatcc tggcctacgt cgagcagctc caggcggtgg acgtcggcgg
     4981 catcgagccg acgagccagg tgaccgaggg caagcccccc gcgctccgcc ccgacgagga
     5041 gcgcccctgc gacgtgcggg acgaagcgct ggcgcaggct cccgaccggg acggcgacta
     5101 cttccgggtg ccgcaagtcg tatgaacgaa tccgtgaagg ggatcggcgc caccgccgag
     5161 gcgatcgccg ccgcggtccg gtctgggagc gccagcgccg aggagatcac gcgggccttc
     5221 ctccagcatg ccgaccagac cgccggcaag ctgggcacgt atctccaccg gatgccggag
     5281 gaggcgctac gcgccgcgcg cgccgtggac gccaagcgcg cctcgggcgc acccctgggc
     5341 gccctggccg gcgtgcccgt ggcgatcaag gacaacatc
//
LOCUS       DASSLZ010000050         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_397761, whole genome shotgun sequence.
ACCESSION   DASSLZ010000050 DASSLZ010000000
VERSION     DASSLZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397761"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VFM00_01800"
     CDS             <1..1291
                     /locus_tag="VFM00_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7497010.1"
                     /translation="RLRGQMAGLYGREFGVAIQDVATTSVLVVALWISARILSRVERA
                     RAQAERGHTESERRLRMMLGVSQVGVWERDVLQNRMECDATSLRLWGFGPDAQPTYVA
                     MIERIHPDDRDRVIREIEKSLERGAEFDTTYRVVHPDRTERALGARGAVTLDPEGRPM
                     RITGINWDVTLHQQAEEAKERAAREQIEMKDQFISHASHELRSPLSSIVEFQDILLRG
                     LVGPPLTPEQRDCVEIAQRNAYQLRLMIDDLLEVTRAQAGKLVVSPQSFAVEGVIEDV
                     VTALKPAANAKMVDLSHSLYEPPPLAFADPARVRQVVTNLVENAIKYTRAGGTVSISA
                     EVDVEGESIEISIEDTGSGISPEDQVWIFEQMYQASRDSRESRKGLGLGLYICRELVT
                     RMGGRIRVDSAVGKGSRFTFTLPLLSAASVQDTRRSA"
     gene            1288..1671
                     /locus_tag="VFM00_01805"
     CDS             1288..1671
                     /locus_tag="VFM00_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7497011.1"
                     /translation="MNGVKTVLIVEDDKDHLKALQIRLGAAGFETIWASDGLQAVAAA
                     RRERPDAIILDLGLPGGDGYQVLQRLKMLAPTSQIPIIVLSARDPAVHRARSLEAGAV
                     AFFQKPADSDALLKTIHESCAPKSA"
BASE COUNT          339 a          710 c          738 g          326 t
ORIGIN      
        1 ccgcctccgc gggcagatgg ccgggctcta cggccgggaa ttcggggtgg cgatccagga
       61 cgtcgcgacc acctcggtcc tcgtggtggc gctctggatc agcgcgcgga tcctctcccg
      121 cgtggagcgg gcgcgcgcgc aggccgagcg gggccacacc gagagcgagc ggcgcctccg
      181 gatgatgctg ggcgtgagcc aggtcggcgt gtgggagcgg gatgtcctcc agaaccggat
      241 ggagtgcgac gcgacgagtc ttcgcctgtg ggggttcggg ccggacgcgc agcccaccta
      301 cgtcgccatg atcgaacgga tccacccgga cgatcgcgac cgcgtgatcc gggaaatcga
      361 gaaatccctc gagcggggcg cggaattcga cacgacgtac cgcgtcgtcc accccgatcg
      421 caccgagcgc gcgctgggcg cgcgcggggc ggtcaccctg gaccccgaag gccgcccgat
      481 gcggatcacc ggcatcaact gggacgtgac cctccaccag caggccgagg aggccaagga
      541 gcgcgccgcg cgcgagcaga tcgagatgaa ggatcagttc atctcgcacg cctcgcacga
      601 gctgcgctcg ccgctttcgt ccatcgtgga gttccaggac atcctgcttc gcgggctcgt
      661 gggtccgccg ctcacccccg agcagcgcga ctgcgtggag atcgcgcagc ggaacgccta
      721 ccagcttcgg ctgatgatcg acgatctcct ggaggtcacg cgcgcgcagg cgggcaagct
      781 ggtcgtctcg ccgcagtcgt tcgcggtcga gggggtgatc gaggacgtgg tcaccgcgtt
      841 gaagcccgcg gccaacgcca agatggtgga tctgtcccac tcgctctacg agccgccgcc
      901 gctcgctttc gccgatccgg cccgggtgcg gcaggtggtg acgaacctgg tcgagaacgc
      961 gatcaagtac acgcgtgccg ggggcaccgt ctcgatcagc gcggaggtgg acgtcgaggg
     1021 ggagagcatc gagatctcga tcgaagacac gggctcgggg atctcgccgg aggaccaggt
     1081 gtggattttc gagcagatgt accaggcgtc gcgggactcg cgcgagagcc ggaagggact
     1141 ggggcttgga ctttacatct gtcgcgaact ggtgacgcgc atgggcgggc ggattcgggt
     1201 ggacagcgcg gtgggaaagg ggagccgctt caccttcacg ctcccgctcc tttcggccgc
     1261 gtcggtgcag gatacgagga ggtcggcatg aacggcgtga agacagtgct catcgtggaa
     1321 gacgacaagg accacctgaa ggcgcttcag atccggctgg gcgccgccgg attcgagacc
     1381 atctgggcca gcgacggact ccaggcggtg gcggctgccc ggcgcgagcg tccagacgcg
     1441 atcattctcg acctgggcct tcccggcggg gacggctacc aggtgctcca acgcctgaag
     1501 atgctcgctc ccacgtcgca gatcccgatc atcgtgctga gcgcccggga tcccgcggtc
     1561 catcgcgcgc ggtcgctgga ggcgggcgcg gtggccttct tccagaagcc ggccgacagc
     1621 gacgcgctgc tgaagacgat ccacgagagc tgcgccccga agtcagcctg acaaacggct
     1681 tcatccgacg ccctcaaagc cacgactccg aaaccccttg acgcgctcgc cccgagcgcg
     1741 cgaggatgcg ccggggtcgt ggcagtgtcc cgcacccgcg ggtatcgcag caccaaacat
     1801 ccttatcttc cgacatcgag actgggcccc aagcacctcg gcttcccatc ctgtccgagg
     1861 gcgcggtctc ttcctatcct cgcgcgctcg gcggcatgga gctccgaacg ggcggggacg
     1921 gcccgtattt tggggaacgc tcgatgcgcc ggaatcgcct cgtgcttctc gccgccgttt
     1981 gcggcgcgct gtccctcgtc ctttcctctc ccgcccgcgc cgacctgact agcaagacgc
     2041 tgaagcccgt cgtcggcgtc aagttcggca tggaatccgc gctcggcagt ctcctcggcg
     2101 gcacgctcgc ggg
//
LOCUS       DASSLZ010000051         4632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1157205, whole genome shotgun sequence.
ACCESSION   DASSLZ010000051 DASSLZ010000000
VERSION     DASSLZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157205"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFM00_01810"
     CDS             complement(<1..321)
                     /locus_tag="VFM00_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET7497012.1"
                     /translation="MESAPQTPSPQASAARVPARDEVLTLLHEHTETPGLRKHALAVE
                     AAMRAYARRMGGDEELWAATGLIHDFDYEKHPTPAEHPFVGVRILEERGWPREMIEAI
                     LGHAS"
     gene            complement(414..962)
                     /locus_tag="VFM00_01815"
     CDS             complement(414..962)
                     /locus_tag="VFM00_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497013.1"
                     /translation="MRRWPLLLVMVPLLAAGCGDRSLILTVDILSFLDPADVSQSYGP
                     IPGGLPLTTVDVDSREVSLLPGVQDVTEVSSAKLDIGASFDNTTGSADARLLVYITSA
                     DSADVFSSAPIADVPVTLTPANVTNVSTEITSDALAAALVQDKVKIGLRIAFDTSASA
                     TPVTGTETITLLRATVITKKKL"
     gene            complement(975..2351)
                     /locus_tag="VFM00_01820"
     CDS             complement(975..2351)
                     /locus_tag="VFM00_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497014.1"
                     /translation="MKRRIPIMLVLLAVAAWTAPAGAQMLDARRLGMGGVATSDNNAS
                     RTANVAFRAVPTGQGSRSIPLPIGLIQYASNHPTFDSSDPDFNIFEILNLATNPPLTL
                     SLWKPKEVSGDISVFVARDSLTVDLSDVRRAIPKDPWKQGGTYHLMAVGFGVKNFFVQ
                     VVPTIHVRNEFDLDPQLRAALRDAQPFTGNTRYGTTDDGVAQAAVAWQAGMTLRPFYR
                     LPVDANEGDQDDPSADDPRRNGATALYLGAAPKYYWGLAYGSIRASAGATTGDTLFGS
                     SDPVAIDMTALTRDAAIGGDGGSGHGFGADVGGVFFYDNFELGVGVNDMGSTIDWKTT
                     VKRHVYDDATNEFTTTTIGRDVETKSRIPAAVTVNVAKRMGATNLAVDFVDTELRTMV
                     HLGVEQWLGMVALRGGAYRDTNGRWQGTAGTGLKMGSVGLDLAVATHSRNVEEERAAE
                     LCASLTLY"
     gene            2576..>4632
                     /locus_tag="VFM00_01825"
     CDS             2576..>4632
                     /locus_tag="VFM00_01825"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET7497015.1"
                     /translation="MTSQTELPKNYDPVETEKRWYAHWSERGYFRPNDSDGPPFVILI
                     PPPNVTGSLHFGHTFDHTIQDLLTRWHRMMGEPTLWLPGTDHAGIATQNVVEKRLAER
                     GQTRFDLGREAFIAETWRWKEEYHRRITEQMKRLGDSVDWSRERFTMDEGLSRAVREV
                     FVRLYRKGLVYRGNRIINWCPRCHTALSDEEVNKEEREAKLYYIQYPIKGTQKHVTVA
                     TTRPETMLGDVAVAVHPKDKRFAPLKGKTALLPFLRREIPIIQDEAVDPKFGTGAVKV
                     TPAHDLSDFEMGQRHNLPAIKVMDESAVMNENAGDFQGLTREEARKRIVEALTDRGLL
                     VRVEPHRHAVGQCDRCGTVVEPYLSLQWFVKMKPLAEPAYAAIARGELTILPRRWEKV
                     YLRWLEGIRDWCISRQLWWGHRIPVWTCSGCEDQIVEVETPTKCPKCGSGELKQDEDV
                     LDTWFSSWLWPFSTLGWPEKTSDLERFYPSSVMVTGPDIIFFWVARMVMAGYEFMGKC
                     PFPQVYLHGIVRDALGRKLSKSLGNSSDPIELMDRFGADSLRYTLVLLSPQGADIHGF
                     SDEKVDLGRHFANKLWNAFRLVHPYLIDFQPDDPVRRPELRDEDQWILSVLASTVRSV
                     TRNLRAYRIGDAAKDIYDFTWREFCDWYLEMAKPRLYAGPHTPDALTVRYVIDSVFST
                     VIRL"
BASE COUNT          719 a         1542 c         1618 g          753 t
ORIGIN      
        1 cgaagcgtgg cccaggatcg cctcgatcat ctcccgcggc cagccgcgct cctcgaggat
       61 ccggacgccg acgaaggggt gctcggcggg agtggggtgc ttctcgtagt cgaagtcatg
      121 gatcaggccg gtggcggccc agagctcttc gtccccgccc atccgtcgcg cgtaggcgcg
      181 catcgcggcc tcgacggcca gggcgtgctt gcgaagtccc ggcgtctcgg tgtgctcgtg
      241 gagcagggtg agcacctcgt cgcgcgcggg aacgcgcgcc gcggaggcct ggggcgaagg
      301 ggtctggggt gccgattcca cgcgcgggat tctactacaa gccgggaagg ggctcttagc
      361 cgtgatctac cgtggtgttt cgtccgcgcc gctttccgcg ccttcggcgc gggttatagc
      421 ttcttcttcg tgatcaccgt ggcgcggagc agcgtgatcg tctcggtgcc cgtgaccggc
      481 gtggcgctcg cgctggtatc gaacgcgatc cgcagcccga tcttcacctt gtcctggacg
      541 agggccgccg cgagggcgtc ggacgtgatc tcggtgctca cgttcgtcac gttggccggc
      601 gtgagcgtca ccggcacgtc ggcaatcggc gccgaggaga agacgtcggc cgagtcggcg
      661 gacgtgatgt agacgaggag ccgcgcgtcg gcggagccgg tcgtgttgtc gaaggaggcg
      721 ccgatgtcca gcttggcgct cgagacctcg gtcacgtcct ggacgccggg gagcagcgac
      781 acctcgcggc tgtccacgtc gacggtggtc aggggaagac ctcccggaat cgggccgtag
      841 gactgcgaca cgtcggccgg gtccaggaag gagaggatgt ccacggtcag gatcaggctc
      901 cggtcgccgc atccggcggc gaggagcggg accatcacga gcagcaaggg ccagcggcgc
      961 atcgggatct cctcctagta cagcgtgagg gaggcgcaga gctcggccgc gcgctcctct
     1021 tcgacgttcc ggctgtgggt ggcgacggcc aggtccagcc cgacgctgcc catcttgagc
     1081 ccggtgcccg cggtgccctg ccagcgcccg ttcgtgtcgc gataggcgcc gccgcgcagc
     1141 gccaccatcc ccagccactg ctccacgccc aggtgcacca tcgtgcgcag ctcggtgtcc
     1201 acgaagtcca ccgcgagatt cgtggcgccc atccgcttgg ccacgttcac cgtgacggcg
     1261 gcggggatgc ggctcttggt ctcgacgtcg cgcccgatcg tggtcgtggt gaactcgttc
     1321 gtggcgtcgt cgtagacgtg ccgcttcacc gtcgtcttcc agtcgatggt gctgcccatg
     1381 tcgttcacgc ccacacccag ctcgaagttg tcgtagaaga acacgccgcc cacgtccgcc
     1441 ccgaagccgt gccccgagcc gccgtcgccc ccgatcgcgg cgtcgcgcgt cagcgccgtc
     1501 atgtcgatcg cgacgggatc ggagctgccg aacagcgtgt cgccggtggt cgcgccggcc
     1561 gaggcgcgga tcgagccgta ggccagcccc cagtagtact tgggcgccgc gcccaggtag
     1621 agcgcggtgg cgccgttccg gcgcggatcg tcggccgacg ggtcgtcctg atcgccttcg
     1681 ttcgcgtcca ccggcaggcg atagaagggg cgcagcgtca tgccggcctg ccatgcgacc
     1741 gccgcctgcg ccacgccgtc gtcggtcgtg ccgtagcggg tgttgccggt gaagggctgg
     1801 gcgtcgcgaa gcgctgcccg aagctgggga tcgaggtcga actcgttccg gacgtggatc
     1861 gtcgggacga cctgcacgaa gaagttcttc acgccgaagc cgaccgccat caggtggtag
     1921 gtgccgccct gcttccaggg gtccttgggg atcgcccggc gcacgtccga caggtccacg
     1981 gtcagggagt cgcgcgccac gaagaccgag atgtcgcccg agacctcctt gggcttccac
     2041 aaggacaggg tcagcggcgg gttggtggcc aggttcagga tctcgaagat gttgaagtcg
     2101 ggatcgctcg aatcgaacgt ggggtggttg ctcgcgtact ggatcagccc gatcggaagg
     2161 ggaatggatc gcgatccctg cccggtcgga acggcgcgga aggccacgtt cgcggtccgc
     2221 gaggcgttgt tgtcgctggt cgccacgcct cccatgccga ggcggcgagc gtccagcatc
     2281 tgcgcgccgg cgggcgccgt ccaggcggcg acggccagca ggacaagcat gatcgggatc
     2341 cggcgtttca tggtcgaggc tcctcccccg gggtttcggg ccgcttttcg gcgggtcccg
     2401 taccccatcc ggcattcatc ggccgatccc gccccgggct tgagtgccgg cgggccgatt
     2461 tgaccgtcga aaacgcgtgt gataacgtag ttcattctct tgaaatcgcg tcagttagcg
     2521 cgacgcgcgc cgcccctcgg cgagcgcatc ccaccaggag gcgatcacgg cgccgatgac
     2581 gagccagacc gagctcccga agaactacga ccccgtcgag accgagaagc ggtggtacgc
     2641 ccactggtcc gagcggggct acttccgtcc caacgactcc gacggacctc ccttcgtcat
     2701 cctgatcccg ccgcccaacg tcacggggag ccttcacttt ggccatacct tcgaccacac
     2761 catccaggac ctcctgaccc ggtggcaccg gatgatgggg gagccgacgc tctggcttcc
     2821 gggcaccgat cacgccggca tcgcgaccca gaacgtggtg gagaagcggc tggcggagcg
     2881 ggggcagacc cgcttcgacc tgggccgcga ggcgttcatc gccgagacgt ggcgctggaa
     2941 agaggaatac caccggcgga tcacggaaca gatgaagcgc ctgggcgact ccgtggactg
     3001 gtcgcgcgag cgcttcacca tggacgaagg gctctcgcgc gccgtgcgcg aggtgttcgt
     3061 gcggctgtac cggaaggggc tggtctaccg cgggaaccgg atcatcaact ggtgtccgcg
     3121 ctgccacacc gcgctctccg acgaggaagt gaacaaggag gagcgcgagg ccaagctcta
     3181 ctacatccag tacccgatca agggcaccca gaagcacgtg acggtggcga ccacgcgccc
     3241 cgagacgatg ctgggcgacg tggccgtggc cgttcacccc aaggacaagc ggttcgcgcc
     3301 gctcaagggg aagaccgcgc tgctgccgtt cctgcgccgc gagatcccga tcatccagga
     3361 cgaggcggtg gatcccaagt tcggaaccgg cgccgtcaag gtgacgcccg cgcacgacct
     3421 ctccgacttc gagatggggc agcgccacaa cctgcccgcg atcaaggtca tggacgagag
     3481 cgcggtgatg aacgagaacg cgggagactt ccagggcctg acgcgcgagg aggcgcggaa
     3541 gcggatcgtg gaggcgctga ccgaccgcgg gctgctcgtg cgcgtggagc cgcaccgtca
     3601 cgcggtgggc cagtgcgacc gctgcggcac cgtggtggag ccctatctct cgctccagtg
     3661 gttcgtgaag atgaagccgc tcgccgaacc cgcctacgcc gccatcgcgc gcggcgagct
     3721 gacgatcctg ccgcggcgct gggagaaggt gtacctgcgc tggctggagg ggatccgcga
     3781 ctggtgcatc tcgcgccagc tctggtgggg ccatcggatc cccgtctgga cctgctccgg
     3841 ctgcgaggac cagatcgtgg aggtggagac acccaccaag tgcccgaagt gcgggagcgg
     3901 ggagctgaag caggacgagg acgtgctcga cacctggttc tcctcctggc tgtggccctt
     3961 ctccacgctc ggctggcctg agaagacgtc cgatctcgag cgcttctatc cctcgagcgt
     4021 gatggtgacc ggccccgaca tcatcttctt ctgggtcgcg cgcatggtca tggccgggta
     4081 cgagttcatg gggaaatgcc ccttcccgca ggtgtacctc cacgggatcg tgcgggacgc
     4141 cctggggcgg aagctctcga agtcgctggg caactcgtcc gatccgatcg agctgatgga
     4201 ccgcttcggc gccgactcgc ttcgctacac cctggtgttg ctctcgcccc agggggccga
     4261 catccacggc ttctccgacg agaaggtgga cctggggcgc cacttcgcga acaagctctg
     4321 gaacgcgttc cggttggtcc atccgtacct gatcgacttc cagccggacg atccggtccg
     4381 ccggcccgag ctgcgcgacg aggaccagtg gatcttgagt gtcctggcct cgaccgttcg
     4441 gtcggtgacg cggaacctgc gcgcctaccg gatcggagac gccgccaagg acatctacga
     4501 cttcacctgg cgggagttct gcgactggta cctcgagatg gccaagccgc ggctgtacgc
     4561 cgggccgcac acgcccgacg ccctgaccgt gcgctacgtg atcgactcgg tgttcagcac
     4621 cgtgatccgg ct
//
LOCUS       DASSLZ010000052         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1158154, whole genome shotgun sequence.
ACCESSION   DASSLZ010000052 DASSLZ010000000
VERSION     DASSLZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158154"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1240
                     /locus_tag="VFM00_01830"
     CDS             266..1240
                     /locus_tag="VFM00_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497016.1"
                     /translation="MPRFPLHAASTALLAALALGAAAPAEPTRVPGTGVRLTPPPGFA
                     PAEQFAGFQNPERNASIVVTEIPGPVSEVRRGMTRSGLASRGVELIESSSVTVGGKEA
                     LLLHVSQRSLGTDYFKWMLVGGSEARTFMVVGTFPAEESDLSDPVRQSLLSASWGEDP
                     RADLFEGMPFRVESAPRLKLADRMGNLLIFTETGRIAPGKPDMAVLIIGSSMGDVAIG
                     DLEGFSKDRLLRTDRMTGVRVRSGASISVDSLPGYELIAEGADAKNGRPVGVYQLMLA
                     DGKSYYIAQGFVAKTRLNQFLPVFRQVTGTFQRTSQEPTEESRPTITQ"
     gene            1324..1626
                     /locus_tag="VFM00_01835"
     CDS             1324..1626
                     /locus_tag="VFM00_01835"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HET7497017.1"
                     /translation="MNRTARTAAAAGFLAFAMAGCAHDPKPAHAITVKGQLAAGAECP
                     MIVTADNRRYSLTGSLDRFKIGDRVCVRGRTVEVSTCMAGEATIAIDAIGPEDNCP"
     gene            1769..1897
                     /locus_tag="VFM00_01840"
     CDS             1769..1897
                     /locus_tag="VFM00_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497018.1"
                     /translation="MERAAAYVALGDTPHARADLAEAARRKAQGSSFAASEDPFRY"
     gene            complement(1925..>3159)
                     /locus_tag="VFM00_01845"
     CDS             complement(1925..>3159)
                     /locus_tag="VFM00_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497019.1"
                     /translation="VGHTLLPIRLSAIYTTGITPPKTSLALVGLVLAAAMVGGFLAWR
                     RKRPVAAFGIALFALSLATVMNVYYTLRIWMTDRYLLFPTIGSSLFLVAMAASLYQAK
                     GARVDPRRAGLRRGLAWAAGLVVVLYAALTVARIGVWTSDLALWSDVVRRQLDLPGSG
                     PVTAAAVAGAAERLPDPGPLVALRRAYDARGNRDEAARLERLADSLSGGEDEHSLMRL
                     ARLDLVAGRYQDALSRLRPVASGRTWFAPLATFRIGVAEEKLGHAEAARQAYDRALAM
                     YREFNQPATDAYFEVGTMEYLAGNYPRAAEWYRLASRESPREADAIFHLALALQMSGR
                     VPEAMALYQRIASGELRFTPHSRMAMPDVYLQMGTGSETMGKKQDAIRYFGEVLRLAP
                     DYPKRDAVRAKIAELSAG"
BASE COUNT          485 a         1146 c         1074 g          454 t
ORIGIN      
        1 atggtctcgg cacgctcccg cacctcggat cgggtgagct tcaccgcttc ggccgccgcc
       61 gtttcgggct ccgccgcgcc cgcggcgcga aggtcccacc ccgcgcccag cccggccagc
      121 gccaggagcg ccgcaatggc cgcagatgtc acggttctca tgggaattcc tcccgttcca
      181 gagtgatggc ccctggaatc atcggcaggt cgggcgaacg cctccagccc tttagttcgg
      241 ccctcgccat gccgataggc aaacaatgcc tcgcttccca ctccacgccg cgtccacggc
      301 ccttcttgcc gcgctcgcct tgggcgccgc ggccccggcc gaacccaccc gcgtgccggg
      361 aaccggcgtc cgcctcacgc cgccgccggg gttcgctccc gccgagcaat tcgcgggctt
      421 ccagaacccc gagcgcaacg cctccatcgt cgtgaccgag atccccgggc cggtctccga
      481 ggttcgaagg ggcatgaccc ggagcggcct cgcctcgcgc ggcgtggagc tgatcgaatc
      541 gagcagcgtg acggtgggag gcaaggaggc gcttctcctg cacgtgagcc agcggtccct
      601 ggggacggac tacttcaagt ggatgctggt gggcggctcc gaagcgcgca cgttcatggt
      661 cgtcggaacg ttccccgccg aagagtccga cctgagcgac ccggtccggc agtcgctcct
      721 gtcggcgtcg tggggtgaag accctcgcgc cgacctcttc gaggggatgc ccttccgggt
      781 ggagagcgcg ccgcgcctga agctcgccga ccggatgggg aatctcctga tcttcaccga
      841 gacgggaagg atcgcgccgg gcaagccgga catggcggtc ctcatcatcg gcagttccat
      901 gggcgacgtg gcgatcggcg acctggaggg cttttcgaag gaccgactgc tccggacgga
      961 ccgcatgacg ggggtgcgcg ttcgaagcgg cgcttcgatc tcggtcgaca gcctgcccgg
     1021 gtacgagctg atcgccgagg gcgcggacgc gaagaacggc cggccggtcg gcgtgtatca
     1081 gctgatgctg gccgacggaa agagctacta catcgcgcag ggcttcgtgg cgaagacccg
     1141 gctgaaccag ttcctgccgg tgttccggca ggtgacggga acgttccagc gtacgagcca
     1201 agaacccacc gaagagagcc gccctacgat cacgcagtag cgcccctaat ccgctggccc
     1261 gagcctcccc ggcggactac cctgagccgg gaagaccgcg accacgcaac gaggaggcgc
     1321 gccatgaatc gaaccgcccg gacggccgcc gcggccggat ttctcgcctt cgccatggcc
     1381 ggctgcgccc acgacccgaa gcccgcgcac gcgatcaccg tcaaaggaca gctcgccgcg
     1441 ggcgccgaat gcccgatgat cgtcacagcc gacaatcgcc gctattccct gaccggcagc
     1501 ctggaccgtt tcaagatcgg cgaccgcgtc tgcgtgcggg ggagaacggt cgaggtctcc
     1561 acgtgcatgg cgggagaagc cacgatcgcc atcgatgcga tcggtcccga ggacaactgc
     1621 ccctgaggac gcctgtccgg atggccgccg ccgttctggc ggcgctcctc gccacggcca
     1681 tccagctcgc cgaccgtccc tatctccgct tcctgcgcgg cctggtgtcc tgccagcgcg
     1741 gcggaggccg gagctgatct atccgctcgt ggagcgggcc gcggcctacg tcgcgctggg
     1801 cgacacgccg cacgcgcgcg ccgatctcgc ggaagcggcg cgccggaaag cgcagggctc
     1861 ttccttcgcc gcgtccgagg atccgttccg ctactgagcg ctacgtaagg ccctggcttg
     1921 tgatctaccc cgccgagagc tccgcgatct tggcgcgcac ggcgtcgcgc ttcgggtagt
     1981 cgggcgccag ccgcagcact tccccgaagt accggatcgc gtcctgcttc ttccccatcg
     2041 tctccgagcc ggtccccatc tggagataga cgtcgggcat ggccatccgg ctgtgcggcg
     2101 tgaagcgcag ctcgcccgag gcgatccgct ggtagagggc catcgcctcc ggaaccctcc
     2161 cgctcatctg gagggccagc gccaggtgga agatcgcgtc ggcctcgcgc ggcgattccc
     2221 ggctggccag gcggtaccac tcggccgctc tcgggtaatt ccctgcgaga tactccatcg
     2281 tcccgacctc gaaataggcg tcggtggcgg gctggttgaa ctcgcgatac atcgcgagcg
     2341 cgcggtcgta ggcctggcgc gccgcctccg cgtgtcccag cttctcctcg gccacgccga
     2401 tccggaacgt cgcgagcgga gcgaaccagg tccgccccga ggcgaccggg cgcagccggg
     2461 acagggcgtc ctggtagcgc cccgcgacca ggtccagccg ggcaagccgc atcaggctgt
     2521 gctcgtcctc tccgccgctc aggctgtcgg ccaggcgctc cagccgcgcc gcctcgtcgc
     2581 gatttcccct ggcgtcatag gcgcgccgaa gcgccacgag cggtcccggg tcgggcagcc
     2641 gctccgccgc gcccgccacg gctgccgcgg tcaccgggcc tgagccgggc agatcgagct
     2701 gccggcgcac cacgtcgctc cacagggcga ggtcgctggt ccagacaccg atccgcgcca
     2761 cggtgagtgc ggcgtagagc acgacgacca gccccgcggc ccacgcgagg ccgcgacgga
     2821 gcccggcccg gcgcgggtcc acccgcgctc ctttcgcctg gtagagcgaa gccgccatcg
     2881 cgaccaggaa gagcgacgag ccgatcgtgg gaaagaggag gtagcggtcg gtcatccaga
     2941 tccggagcgt gtagtagacg ttcatcaccg tcgcgagcga gagcgcgaag agcgcgatcc
     3001 cgaacgccgc cacggggcgc ttgcggcgcc aggccaggaa tccgcccacc atcgccgcgg
     3061 ccaggacgag gcccaccagg gcgagcgagg tcttgggcgg cgtgatcccc gtcgtgtaga
     3121 tggccgagag gcggatcgga aggagcgtat gaccgacat
//
LOCUS       DASSLZ010000053         7476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_652531, whole genome shotgun sequence.
ACCESSION   DASSLZ010000053 DASSLZ010000000
VERSION     DASSLZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652531"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1039
                     /locus_tag="VFM00_01850"
     CDS             14..1039
                     /locus_tag="VFM00_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497020.1"
                     /translation="MDRAIPISVPEGTLAGAESARAESLTVAENLARGAADPEHALQA
                     ARIFSLEGKRDRAIEILRQGTLAHPRHVGLLGTLADLLSRGGAFEEADLVFRQALEYG
                     PGDAEAWYRAGLHGARQGQLESARSAYEETVRLDPTRVRAWVNLGLARADLGDRRGAL
                     QALLRAVQADPACAEAHSNLGVLYHEEGMRAEAIEAFRRSVELAPDSSEAHFNLGWAL
                     LADQELEQSETMLEASVRLDPSNVESLYALALLHIRVGKYGRAVASLRQAVEHQPDDA
                     RLHYQLGVAYNRQDAVDQAILALETAARLNPDDPRPHHLLGVAYDKKELPALAREAYR
                     RAAALNG"
     gene            1042..1848
                     /gene="trpA"
                     /locus_tag="VFM00_01855"
     CDS             1042..1848
                     /gene="trpA"
                     /locus_tag="VFM00_01855"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET7497021.1"
                     /translation="MPGVGRAFEAARRERRGAFIAYLASGYPNLAESNRLAAAACDAG
                     ADVLELGVPFSDPVADGPVIQRATQVALEGGATLASVIRQAATLRAAHATPIVLMSYL
                     NPIHRYGVERFARDAAASGVDGVILVDLPPEEDPETWETVVEAGLDTIALVTPTSAAH
                     RLPAIASRSRGFVYVVARLGVTGAGGADPDTAALLDRCRALSPLPRAIGFGMSLESDL
                     APYRGRAEGVIVGSGLLQPMLGEPDAAARERGLRERVRAFRKKVEDLGGA"
     gene            complement(1902..2849)
                     /locus_tag="VFM00_01860"
     CDS             complement(1902..2849)
                     /locus_tag="VFM00_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497022.1"
                     /translation="MTPRTSWNPKRWMPAFAAALGLFLAGCDSGQIAAPVSTGVQWTR
                     ITNPATAKLAKYPDWRGNRIAFAYLVGTKVNIALCQPDGSGLSYLSEGASENQLQPRW
                     ADDSTLIYTLTSSGTTSYDLWRRIITTDEIRQLTAFTGDELSPAPRPGTTGLAYTEQA
                     AKGRIVLIPDYTESTPTRLYLTGTGLDCGEPDWDPAGQRICFTVDSTSSYRHVWMVTL
                     APGDSTPVQLTTGPYFDTGPRFSPDGSRILFASSKRTGRPGLWTIAPSAGMPSLRLVA
                     FDDAGAVVATPCWSPDGTKVVTVSTGRGDQALWLLSNLP"
     gene            3170..3340
                     /gene="csrA"
                     /locus_tag="VFM00_01865"
                     /pseudo
     CDS             3170..3340
                     /gene="csrA"
                     /locus_tag="VFM00_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011714646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
     gene            3459..3794
                     /locus_tag="VFM00_01870"
     CDS             3459..3794
                     /locus_tag="VFM00_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497023.1"
                     /translation="MRTVKAPREPENEEWLVRGEEVTALFDCALRYVGEGNRSVAERS
                     VLELMRCLNSGGRGAGARVLAHFDACLAQLERGDFDDAFENLLMLKDSWVEALAELRR
                     DRTRASNVN"
     gene            3775..3960
                     /locus_tag="VFM00_01875"
     CDS             3775..3960
                     /locus_tag="VFM00_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497024.1"
                     /translation="MPQTSTKRRSESKSDLLESETVESTLERARRRIREGYYDRAEVR
                     RTLATLILHRAARKTLA"
     gene            4077..5273
                     /locus_tag="VFM00_01880"
     CDS             4077..5273
                     /locus_tag="VFM00_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7497025.1"
                     /translation="MRPRPATDLSPTTSSPPRPARAVAESLYRYAPFGVLAVDREGRI
                     VAANETACRLLGYGVEEMLQEPAALYFRAPKGDGHVLPGACAAPEEPREVEVATKNQD
                     ALPVSLRLLPLETESGALGGTLALFVDLRGEKSRDEQWRRRDRLASLGALAAGVAHEI
                     RNPLAGIGASAQILARRLPAGDARASFATLIVEEVARLDRIVESLLQFARPATPKLAR
                     QSLLPALEKAITLMHEPAVRQKVSLVADRAERVPEIYVDVDQILQVIVNVVKNAIQAM
                     PDGGEIKVGMGVKRKRLVERSAIGRRASDRLESARPIPEQEVVEIQVQDNGPGIPAAT
                     LARVFDPFFTTRAQGSGLGLSICQTIVREHGGVIAVESTVGQGTTVTIDLPVEKRHGD
                     RRTDPR"
     gene            5245..6666
                     /locus_tag="VFM00_01885"
     CDS             5245..6666
                     /locus_tag="VFM00_01885"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7497026.1"
                     /translation="METDAPILVKPTAGVLVVDDERSLRFTLSESLSEEGYRVDTASD
                     GAEALSKVEGGDFQLVLLDQKLPDANGIEVLRKIKTKRPELPVVIMTAYGKFENAVEA
                     ARAGCYDYIGKPFELDHIKLVLANALNQSRLVDEVTRLRAADRRRAGTALVSGGSPSM
                     EKIFDTMRKIAQSGSSTILLEGETGVGKELVARETHDLSQRREGPFVPVNCAAIPESL
                     LEGELFGAEPGAYTDSKKRKRGFLEQANGGTLFLDEIGEMQASLQARLLRALEQRRFR
                     RLGGETEVAVDLRVIAATNRDLRLMVDQGKFREDLYFRLAVIRVPVPPLRDRTGDIQA
                     LIDHFVEHFNKEFGKRVKGPNEEALALMLAYRWPGNVRELKNVIERAVLLESEEWILP
                     EHLPLEIVGAGGSAPKVLETRLHVDAGVLTLAKAEQIAIEMALAQAGGNKTRAAEALG
                     ISRQTLRTKLKEYRMEAPGGHDG"
     gene            6656..>7476
                     /locus_tag="VFM00_01890"
     CDS             6656..>7476
                     /locus_tag="VFM00_01890"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET7497027.1"
                     /translation="MTAEPVQVSGNDPRRPPQPNPFRLERGGPASPRAGLLTTGHGVV
                     RTPAFMPVGTAGSVKGLSPDEIRAAGTQILLANTYHLMLRPGAELVRELGGLHAFMGW
                     DGAILTDSGGYQMVSLKERVRISEEGVEFRSHLDGRLEFLSPERAMEIQADLGPDIAV
                     TLDHAVPLPAPEAEVRDGAERTLRWTERSLARAASLTTPTGLPQLHFAIVQGGGDPAL
                     RAEMAARTAALPCDGFALGGLSLGETKAETWASARGANEALPADRPRYLMGMGTP"
BASE COUNT         1146 a         2605 c         2681 g         1044 t
ORIGIN      
        1 ctaggctgcg gccgtggatc gcgccattcc catctccgtc cccgaaggaa ccctcgccgg
       61 cgccgaatcg gcgcgggccg agagtctcac cgtcgccgag aacctcgccc gcggcgccgc
      121 cgaccccgag cacgcgctgc aggcggcgcg catcttctcg ctcgaaggga agcgcgatcg
      181 cgcgatcgag atcctccgcc aggggacgct ggcccatccg cggcacgtcg gcctcctcgg
      241 gaccctcgcc gatctcctct cccgcggagg cgcgttcgag gaagcggacc tcgtgttccg
      301 gcaggcgctc gaatacggcc cgggcgacgc ggaagcgtgg tatcgcgcgg ggctgcacgg
      361 cgcgcgccag ggacagctgg agtcggcacg cagcgcctac gaggagaccg tgcgcctcga
      421 tccgacccgg gtgcgggcct gggtgaacct gggactggcg cgggccgacc tgggcgaccg
      481 ccggggcgcg ctccaggctc tgctgcgcgc ggtgcaggcc gatcccgcct gcgccgaggc
      541 gcattcgaat ctcggcgtgc tctatcacga agaggggatg cgggccgagg cgatcgaggc
      601 gttccgccgc tccgtggagc tcgctcccga ttccagcgag gcgcacttca acctgggctg
      661 ggcgctcctc gccgaccagg agctggagca gtccgagacg atgctcgagg cttcggtgcg
      721 gctcgacccc tcgaacgtgg agagcctgta cgcgctggcg ctgctccata tccgcgtcgg
      781 gaagtacggc cgcgcggtcg cctcgctccg gcaggcggtc gagcaccagc ccgacgacgc
      841 gcgcctccac taccagctgg gggtcgccta caaccgccag gacgccgtgg accaggcgat
      901 cctcgcgctg gaaaccgccg cccggctgaa tcctgacgat ccccgtccgc accatctgct
      961 cggcgtcgcc tacgacaaga aagagctccc cgcgctggcg cgcgaggcct atcggcgcgc
     1021 cgccgcgctg aacggctgac cgtgccgggc gtcgggcgcg cgttcgaggc ggcccggcgc
     1081 gagcgccgcg gcgccttcat cgcctacctc gcctcaggct atcccaacct cgcggaatcg
     1141 aaccgcctcg cggccgccgc ctgcgacgcg ggcgccgacg tgctcgagct gggcgtcccg
     1201 ttttccgatc ccgtcgccga cggcccggtc atccagcgcg cgacgcaagt cgctctggag
     1261 ggcggcgcga ccctcgcgag cgtgatccgg caggccgcca ccctccgtgc ggcgcatgcc
     1321 accccgatcg tgctcatgtc ctacctgaac ccgatccacc gctatggggt ggagcgcttc
     1381 gcgcgggacg ccgcggcttc gggcgtggac ggcgtgatcc tggtggacct cccgcccgag
     1441 gaggatccgg agacgtggga aacggtggtg gaagcgggac tggatacgat cgcgctcgtg
     1501 accccgactt cggcggcaca tcgccttccc gcgatcgctt cgcgctcgcg gggatttgtg
     1561 tacgtggtcg cgcgcctcgg cgtcaccggc gccgggggcg ccgatcccga cacggcggcg
     1621 ctcctcgacc gctgccgcgc gctctcgccc cttccgcggg cgatcggctt cggcatgtcg
     1681 ctcgagagcg acctcgcgcc ctatcgcggg cgcgccgagg gggtcatcgt gggctcgggg
     1741 cttctccagc cgatgctggg cgagccggac gccgccgcgc gcgagcgcgg gctgcgcgag
     1801 cgggtgcgcg cgttccggaa gaaggtggag gacctgggcg gagcttagcg gcgaaggcgc
     1861 actttggccg gggcgctacg gcacgaggcc cggcgcgaga tctacggtag gttcgaaaga
     1921 agccagagcg cctgatcgcc gcgccccgtg gagacggtga ccaccttggt gccgtccggg
     1981 gaccagcagg gtgtggcgac cacggctccg gcatcgtcga acgcgaccag ccggagcgag
     2041 ggcatgccgg ccgacggcgc gattgtccac aggccggggc gcccggtgcg cttgctcgaa
     2101 gcgaacagga tgcgcgagcc gtcggggctg aaccgggggc ccgtatcgaa gtagggtccc
     2161 gtggtcagct ggaccggcgt gctatcgcca ggcgccaacg tgaccatcca gacatggcga
     2221 tagctgctgg tgctgtccac ggtgaagcag atccgctgac ccgccggatc ccagtccggc
     2281 tcgccacagt ccagcccggt accggtcaag tagaggcgtg tcggcgtgga ttcggtgtag
     2341 tcgggaatga gaacgatgcg ccccttcgcc gcctgttcgg tgtaggcgag ccccgtcgtc
     2401 ccgggacgcg gggcagggct cagctcatcg ccggtgaacg cggtcagctg ccggatctcg
     2461 tccgtcgtga tgatccgcct ccagaggtcg tagctggtcg tgccgctgga tgtcagcgtg
     2521 tagatgagcg tcgagtcgtc cgcccatcgc ggctggagct ggttctccga cgccccctcg
     2581 gacaggtagg acagcccgct gccgtcgggc tggcatagag cgatgttcac cttggtgccg
     2641 accaggtagg cgaacgcgat ccgattgccg cgccagtccg gatacttggc cagcttggcg
     2701 gtcgcggggt tggtgatccg cgtccattgg acccccgtcg agacaggggc cgcgatctgg
     2761 cccgagtcgc agcccgccag gaagaggccc agcgccgccg cgaaagcggg catccagcgc
     2821 ttcgggttcc aggatgtgcg aggggtcacg ccgctcagtc tatccgctcg gacgctcatt
     2881 ctcaagatat cggcccggcc cgcccgcacc tgagtccgga cgcgcgggtc cggccgcgtg
     2941 gctcgatcgg accgccgcgc gcgcgagccg atgcctgcgc atgcgccccc gcgcgcggca
     3001 ttccggcacc tctccggacc ttcccgcacg ccctccgcga cctttccggg ttcagatgcc
     3061 cacccccact gccgatcaca ggaacgaggt ccgagcggcc ccgcgcaacg aggcgcgacg
     3121 gtcgtgacgg aaaccccgtc cggcaggaag ccggaggtgc gagggcacga tgctcctctt
     3181 gacgcgaaag ctcggcgaga acatccggat cggtgacgac gtcaagatca ccatcgtgga
     3241 ggtgaaagga aaccacgtga agctgggcat cgacgcgccg ccgagcgtga aggtgcaccg
     3301 cgaagagatc tacgaacgga tccagcagga gaaccgaagg aaccaggccc aggccgcggc
     3361 ccagaacccg ggcacgacgc cgggaagcgc gggaacggat tcctcccggt ccgtgaacgg
     3421 atcggacggg gcgcctcacg ccacggaggg gaagggcgat gcgaacggtg aaggcgcctc
     3481 gggagcctga gaacgaggag tggctggtcc gcggcgaaga ggtcaccgcg ctcttcgatt
     3541 gcgccctccg ctacgtcggc gaggggaatc gctccgtggc cgagcgctcg gttctcgagc
     3601 tgatgcgctg cctcaactcc ggcgggcgcg gcgcgggggc gcgggtcctg gcgcacttcg
     3661 acgcctgcct ggcacagctg gagcggggcg atttcgacga tgccttcgag aatctcctga
     3721 tgctgaagga ttcctgggtg gaagcgctgg cggagctccg gagggaccgc acccgtgcct
     3781 caaacgtcaa ctaagcgccg ctccgaatcg aagagcgacc tgctcgagtc cgagaccgtg
     3841 gaatcgaccc tggagcgcgc ccgtcgccgc atccgcgagg gctattacga ccgggccgag
     3901 gtgcgccgca ccctggctac gctgatcctc catcgcgccg cgcggaaaac cctcgcctga
     3961 ttccgcttga cccctcccga gggccgctgg taaagatgta gccgctcccg taaccagaat
     4021 ccacaagcgg agattggctc agggatgacg ccgacgatcg gcttggacgc cgcttcgtga
     4081 gaccccgccc cgccaccgat ctttccccga cgacctcgtc cccgccgcgc cccgcgcgcg
     4141 ccgtggccga gagtctctat cgctacgcgc ccttcggggt tctggcggtg gaccgcgagg
     4201 gacggatcgt cgcggccaac gagaccgcct gccggctcct cggctatggc gtcgaggaga
     4261 tgctccagga gccggccgcg ctctatttcc gggcgccgaa aggggacggg cacgtccttc
     4321 cgggcgcctg cgccgcgccc gaggagccgc gcgaggtgga ggtggcgacc aagaaccagg
     4381 acgccctccc ggtctcgctc cgcctgctcc ccctcgaaac cgagtccggc gcgctcggag
     4441 gcacgctcgc gctcttcgtg gaccttcgcg gcgagaagtc ccgcgacgag cagtggcggc
     4501 gccgggaccg gctggcctcg ctgggggcgc tggccgccgg cgtggcgcac gagatccgga
     4561 acccgctcgc ggggatcggc gcctcggcgc agatcctcgc ccggaggctt cccgcggggg
     4621 acgcgcgcgc ctccttcgcc accctgatcg tcgaggaggt ggcgcgcctc gaccggatcg
     4681 tggagagcct gctccagttc gcgcgccccg ccacgcccaa gctggcccgg cagtcgctgc
     4741 ttcccgctct ggagaaggcg atcacgctga tgcacgagcc ggcggtgcgc cagaaggtgt
     4801 cgctggtcgc cgaccgcgcc gagcgcgtcc cggagatcta cgtcgacgtc gaccagatcc
     4861 tccaggtgat cgtgaatgtc gtgaagaacg cgatccaggc gatgcccgat gggggcgaga
     4921 tcaaggtcgg gatgggcgtg aagcggaagc ggctcgtgga gcggagcgcg atcggccggc
     4981 gcgcctccga ccggctggaa tcggcgcgcc cgatcccgga gcaggaagtc gtcgagatcc
     5041 aggtgcagga caacggcccc ggaattccgg ccgccacgct ggcccgcgtc ttcgatccct
     5101 tcttcacgac ccgggcccag gggagcgggc tggggctttc catctgccag accatcgtgc
     5161 gcgagcacgg aggcgtgatc gccgtcgaga gcaccgtcgg acaggggacc accgtcacga
     5221 tcgaccttcc cgtggagaaa cgacatggag accgacgcac cgatcctcgt taagccgacc
     5281 gccggcgtgc tcgtggtgga cgacgaacgc tcgctccgct tcacgctgag cgagagcctc
     5341 tcggaagagg gctaccgcgt cgatacggcg tcggatggcg ccgaggcgct ctccaaggtc
     5401 gagggcgggg acttccagct ggtcctgctc gatcagaagc ttcccgacgc caacgggatc
     5461 gaggtgctcc ggaagatcaa gacgaagcgg ccggagcttc cggtggtgat catgaccgcc
     5521 tacggcaagt tcgagaacgc ggtcgaggcg gcgcgcgccg ggtgctacga ctacatcggg
     5581 aagccgttcg agctggacca catcaagctc gtgctcgcca acgcgctgaa ccagtcgcgg
     5641 ctggtggacg aggtgacgcg gttgcgagcc gccgaccggc ggcgtgcggg cacggcgctc
     5701 gtctccggcg gcagcccctc catggagaag atcttcgaca ccatgcggaa gatcgcccag
     5761 agcgggagca gcaccatcct gctcgagggc gagacgggag tggggaaaga gctggtcgcg
     5821 cgcgagaccc acgatctgag ccagcggcgc gagggcccct tcgtgccggt gaactgcgcc
     5881 gcgattcccg agtccctgct cgagggcgag ctgttcggcg ccgagcccgg cgcctacacc
     5941 gattccaaga agcggaaacg cgggttcctg gagcaggcga acggcggcac cctgttcctg
     6001 gacgagatcg gggaaatgca ggccagcctc caggcgcgcc ttctgcgcgc actggaacag
     6061 cggcgcttcc gccgtctcgg cggcgagacg gaggtggccg tggacctccg cgtgatcgcc
     6121 gccacgaatc gcgatctgcg tctcatggtg gaccagggga aattccggga agatctctac
     6181 ttccgtctcg ccgtgatccg tgtgccggtg ccgcccctcc gcgaccggac cggagacatc
     6241 caggcgctga tcgaccactt cgtggagcac ttcaacaagg aattcgggaa gcgcgtgaaa
     6301 gggccgaacg aggaggcgct cgcgctgatg ctggcctacc gctggccggg gaacgtgcgc
     6361 gagctgaaga acgtgatcga gcgcgccgtc ctcctcgaga gcgaagagtg gatcctgccg
     6421 gagcacctgc cgctcgagat cgtgggcgcg ggcggctcgg cccccaaggt gctggagacg
     6481 cggctgcacg tggacgcggg cgtcctcacg ctggccaagg ccgagcagat cgcgatcgag
     6541 atggcgctgg cgcaggcggg cggcaacaag acgcgcgcgg cggaggcgct cgggatctcg
     6601 cgccagacgc tccgcacgaa gctgaaggag taccggatgg aagccccggg gggtcatgac
     6661 ggctgaaccg gtgcaggtgt ccggtaacga cccccggcgg cctccccaac cgaacccatt
     6721 ccgcctggag cgcggcggcc ccgcctctcc gcgcgccggg ctcctgacca ccgggcacgg
     6781 cgtcgtgcgc acaccggcgt tcatgccggt cggcacggcc gggagcgtga aggggctttc
     6841 tcccgacgag atccgcgcgg ccggcacgca gatcctcctc gcgaatacct accacctgat
     6901 gctccgcccg ggcgccgagc tcgtgcggga gctgggcggc ctccacgcct tcatggggtg
     6961 ggacggcgcc atcctcaccg acagtggcgg ctaccagatg gtcagtttga aggagcgcgt
     7021 gcggatctcc gaggaggggg tggagttccg ctcgcacctg gacggacgcc tggagttcct
     7081 ctcgcccgag cgggcgatgg agatccaagc cgatctcggg cccgacatcg cggtcacgct
     7141 ggatcacgcc gtaccgctcc cggcgccgga ggccgaggtg cgcgacgggg cggagcggac
     7201 gctccgctgg accgagcgct cgctggcccg cgcggcgtcg ctcaccacgc ccaccgggct
     7261 tccgcagctc cacttcgcga tcgtgcaggg cgggggcgat ccggcgcttc gagcggagat
     7321 ggccgcgcgc acggccgcgc tcccgtgcga cgggttcgcg ctcggcggac tctccctggg
     7381 ggagaccaag gccgagacgt gggcctcggc gcgcggcgcc aacgaagcgc tgccggcgga
     7441 ccggccccgc tacctgatgg gcatgggcac gcccga
//
LOCUS       DASSLZ010000054         4726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_780617, whole genome shotgun sequence.
ACCESSION   DASSLZ010000054 DASSLZ010000000
VERSION     DASSLZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780617"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VFM00_01895"
     CDS             complement(<1..766)
                     /locus_tag="VFM00_01895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7497028.1"
                     /translation="MKPDHYALGRSERETRRLILQHQIYGPVTRRFFEAAGIGAGMKV
                     LDIGSGAGDVAILLADLVGPRGRVVGVDMNPEILETARARAAAAGWRNIEFLAGDALH
                     LDLDRDFDVVAGRWVLMHVPDPVALLRHLVTRLRTGGIVAFHENDFTYPPTVLPPTEL
                     SAQIQRWSIHPCEGPGAEMRMGTKLLRTFVAAGLPEPQFLVEAPAGGGSDWPGYEYVA
                     DTLRSLMPMLQQRAGVDPDEVQVDTLADRLRADVASV"
     gene            826..1137
                     /locus_tag="VFM00_01900"
     CDS             826..1137
                     /locus_tag="VFM00_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497029.1"
                     /translation="MLILREIMYCKPGKVRPLVEKFLAMSKLNEKMGMGKMRIMTDVA
                     ADRYWMLVAEMEVESLSKFEAMMAGEGLNEADMKEFEKIMGNYHELVDHGRREIYKLE
                     G"
     gene            complement(1119..2075)
                     /locus_tag="VFM00_01905"
     CDS             complement(1119..2075)
                     /locus_tag="VFM00_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7497030.1"
                     /translation="MLDVLDARLVHQRTGRRRTICAIVRVAGPGGVRSEETWYAKEYS
                     SSKGARSYRKQMAIRNAVRHGVSVPEPLGYSVEDRLLVMREVPCRSLEALLRDPARDL
                     SAVLDHAGEALNGLHELETRMLPPDASGAFGAHGPREESLVLRAAGARVEGAPFEGTL
                     RMRFQEALETTIVCLGSDPPSNPGLLHRDLHPAQVVPVERGIVLLDTDETAFGEPELD
                     VGNLAAHLTLEAIRRSAGSDDGGVWAAALTSGYSRKRALQGKRLGLYTAAALLRLASL
                     ERVARADVSRLDWPRLANALVDAAQRELSAVTRTRPSYPSSL"
     gene            complement(2174..4072)
                     /locus_tag="VFM00_01910"
     CDS             complement(2174..4072)
                     /locus_tag="VFM00_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7497031.1"
                     /translation="MIERIRAALATFRQGARDSRRYLSALARLRPYVHPHRGQLLLSC
                     FAALGYASMTLLEPWPIRFLIDGVLLHHKVRLVGFKVAPHADPSTMILLSVGLVVLFA
                     ALRGQLYYVQNVATATAGQDVVMSLRRELFEHLQSLSIAYHRRGRLGDVLMRLTGDIV
                     MLRDMVVSALVSVLAQGLVLVGVLVVMAAINLKLMLVALAVTPLLFLIISVFRVRLQE
                     AASEQRKREGRLVSSMQEVIQSIHVVQANTAEPYEADRFRMMNQRSLRAGIRSTRLEA
                     KLHRATQMTIAGGIGATLAVGAHDVLAGRLTPGQLIVFLAYVRGLYGPMRQVSKVVQR
                     SAKASACADRVLEVLDERPEIASPPNAPPLRVPEGRVVLDRVTFGHDPDAPVLHDVTL
                     EAPPGTTTAIVGPTGSGKTTLLNLIPRFFDPQRGEVRIDGTSVRVVSLRSLRGHISYL
                     TQETVVMGVTIRENIAYGALGQDHGAPTIEEIEQAARFAHAHDFIADLPGGYDTVIGE
                     RGATLSGGQRQRIAIARALLREAPILLFDEPMTGLDPLAERAVREAFITLTRDRTTLV
                     VAHQLETVLHADQIFFLENGRIVERGTHADLIAAGGRYAAFCRAHWSGVKRGEPAGPS
                     RAAAGADS"
     gene            complement(4069..>4726)
                     /locus_tag="VFM00_01915"
     CDS             complement(4069..>4726)
                     /locus_tag="VFM00_01915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7497032.1"
                     /translation="FRRPQGRPVSPCPGDPVMVREETPTVLAVGRLVEKKGFGVLIDA
                     MSVLLHRGMRARAWIVGDGPERGRLERRIDELGLGSTVQLLGPVAQDRLIELFDRAHL
                     LAQPCIVAADGDQDGIPTVILEAMAVGLPVVTTPLSGIPEAVQDGATGLHVPCGDAGA
                     LADAIRRLIADDALAARLAAEARRAVEERFDLGNSARELVQLFRWSAHDAWRPPENGS
                     "
BASE COUNT          783 a         1566 c         1679 g          698 t
ORIGIN      
        1 ggaccgacgc gacgtccgcg cgaaggcggt cggccagcgt gtccacctgg acttcgtcgg
       61 ggtcgacgcc ggcccgctgc tgcagcatcg gcatcaggct gcgcagcgtg tccgccacgt
      121 attcgtagcc cggccagtcg ctgccgcccc ccgcgggcgc ctccaccagg aactgaggct
      181 ccgggagacc cgcggccacg aaggttcgga gcagcttggt gcccattcgc atctcggctc
      241 cagggccctc gcagggatgg atgctccagc gctggatctg ggccgagagc tcggtcggcg
      301 ggagcaccgt tggcggatag gtgaagtcgt tctcgtgaaa ggcgacgatc ccgccggtcc
      361 ggagccgcgt caccagatgc cgcagcaggg ctaccggatc gggcacgtgc atgagcaccc
      421 agcgtccggc aaccacgtcg aaatcgcggt cgaggtccag gtgcagtgcg tcgccggcga
      481 ggaactcgat gttgcgccag cccgccgcgg cggcgcgggc gcgggccgtc tcgaggatct
      541 cgggattcat gtccaccccc accacgcgcc cgcgtggacc cacgagatcc gcgagcagga
      601 tggccacgtc cccggcgccg ctgccgatgt ccagcacctt catgccggcg ccgatccccg
      661 ccgcttcgaa gaaccggcgg gtcacgggtc cgtagatctg atgctgcagg atcaagcggc
      721 gggtttcgcg ctcgctccgt cctagtgcat agtggtcggg cttcacgttc gaatccatgg
      781 cgtcaaggtt acgaggcccc gacaattcgt cacaaggaga gaatcgtgct gatcctgaga
      841 gagatcatgt actgcaagcc ggggaaggtg cgtcccctcg tcgagaagtt cctggcgatg
      901 tcgaagctca acgagaagat gggcatgggc aagatgcgca tcatgaccga cgtcgccgcc
      961 gatcgctact ggatgctggt tgccgaaatg gaagtcgaga gcctgagcaa gttcgaggcg
     1021 atgatggcgg gggagggctt gaacgaggcg gacatgaagg agttcgagaa gatcatgggg
     1081 aactaccacg agctggtgga tcacgggagg cgggagatct acaagctgga agggtaggac
     1141 ggtctcgtcc gggtcaccgc cgagagctcg cgctgggccg cgtcgaccag tgcgttcgcc
     1201 aagcgcggcc agtccagccg gctcacgtcc gcacgggcca cccgctccag cgaggcgagc
     1261 cggagaagcg ccgcggccgt gtagaggccc aaccgcttcc cctggagggc gcgcttgcgg
     1321 gaatagcccg aggtaagcgc cgcggcccac acgcccccgt cgtccgaacc cgccgagcga
     1381 cggatcgcct cgagggtcag gtgcgcggcg agatttccga cgtcgagctc gggctcgccg
     1441 aaggcggtct cgtccgtgtc gagcagcacg atgcctctct cgacgggaac gacctgcgcg
     1501 ggatggaggt cgcggtgcag gaggcctggg ttcgagggcg gatcgctgcc caggcagacg
     1561 atcgtcgtct cgagggcttc ctgaaagcgc attcgaaggg tcccttcgaa gggcgccccc
     1621 tcgacgcggg ccccggccgc tctcaggacg agcgactctt cacggggccc atgcgcgccg
     1681 aacgctccgg aggcatcggg cgggagcatc cgggtctcca gctcgtgcag tccattcagg
     1741 gcctcgccgg catggtcgag cacagcggac aggtctcgag ccggatcgcg gaggagcgcc
     1801 tcgagggaac gacacgggac ctcgcgcatc accaggagcc ggtcctcgac cgaatacccc
     1861 agcggttccg gaaccgacac gccgtgccgc acggcgttcc ggatcgccat ctgcttccgg
     1921 tacgaccggg ctcccttgga gctcgaatac tccttggcgt accaggtctc ctcggagcgg
     1981 acgcctccag gtcccgctac gcggacgatc gcgcaaatcg tccgccgccg cccggtgcgc
     2041 tggtgcacca gtcgggcgtc cagcacgtcg agcacgcgcc ggccggattc ggcgaccgcg
     2101 cgattcagcg actcgcgtaa gggcgcgacc ggaaccgcgg cgggcgattt cccctcgttc
     2161 ggtctcggcg agctcagctg tcggctcccg cggcagctcg cgacggacca gcgggctcgc
     2221 cccgtttcac tccactccag tgggcgcggc agaaagcggc atagcggccg cccgcggcga
     2281 tcagatcggc atgggtgcct ctctctacga ttcgcccgtt ctccaggaag aagatctgat
     2341 cggcatgcag cacggtctcg agctggtgcg cgacgaccag agtggtccgg tcccgcgtga
     2401 gcgtgatgaa cgcctcgcga accgcgcgct cggcgagcgg atcgagcccc gtcatgggct
     2461 cgtcgaagag caggatcggc gcttcgcgca ggagcgcgcg cgcgatggcg atgcgctgcc
     2521 gctgtccgcc ggagagcgtc gcgccgcgct cgccgatcac ggtgtcgtat ccgccgggca
     2581 ggtccgcgat gaagtcgtgg gcgtgcgcga agcgggcggc ctgttcgatc tcctcgatcg
     2641 tgggagcgcc gtgatcctgc ccgagcgcgc cataggcgat gttctcgcgg atagtgacac
     2701 ccatcacgac ggtctcctgg gtgagatagc tgatgtgccc ccgcagcgat cgcagcgata
     2761 ccactcgaac cgaggtgccg tcgattcgca cctcgccccg ctgggggtcg aagaagcgtg
     2821 ggatgaggtt gagcagggtc gtcttgccgc ttcccgtggg tccgacgatg gccgtcgtcg
     2881 tgccgggagg cgcctcgagg gtcacgtcat ggagcaccgg tgcgtcgggg tcgtgtccga
     2941 aagtgacgcg atcgagaacc acgcgtcctt ccggtacccg gagcgggggc gcgttcgggg
     3001 ggcttgcgat ctcagggcgc tcgtcgagca cctcgaggac ccggtccgcg caggccgagg
     3061 ctttcgcgct tcgctgcacg actttggaga cctggcgcat gggcccgtag agaccgcgaa
     3121 cgtacgcgag gaacacgatc agctgtcccg gcgtgagccg gcccgcgagc acgtcgtgcg
     3181 cgcccacggc cagggtggcc ccgatcccgc ccgcgatggt catctgggtg gctcggtgga
     3241 gcttggcctc gagccgcgtg gaccggattc ccgcgcgcag gctccgctgg ttcatcatgc
     3301 ggaagcggtc tgcctcatag ggctcggccg tgttggcctg cacgacgtgg atcgactgga
     3361 tgacctcctg catgctcgat accagccgcc cttcgcgctt gcgctgctcg gaggcggctt
     3421 cctgcagacg cactcgaaat accgagatga tcaggaagag aagcggcgtc accgccagcg
     3481 cgacgagcat cagtttgaga ttgatggcgg ccatcacgac gagcacgccc acgagaacga
     3541 gcccctgagc caggacgctc accagcgcgg aaacgaccat gtcgcggagc atcacgatgt
     3601 cgccggtgag acgcatgagc acgtccccga ggcggccgcg ccggtgatag gcgatcgaca
     3661 gggactggag gtgctcgaac agctcgcgtc gaagggacat gacgacatcc tgcccggcgg
     3721 tcgccgtcgc gacgttctga acgtagtaga gctggccgcg aagggccgcg aacagcacga
     3781 ccagtccgac cgagagaagg atcatcgtgc ttgggtcggc atgaggcgcc accttgaatc
     3841 cgacgaggcg gaccttgtga tgcaggagca cgccgtcgat caggaaccgg atcggccaag
     3901 gctcgagaag cgtcatcgac gcatagccga gcgccgcgaa gcaggagagg aggagctgtc
     3961 cccggtgcgg atgcacgtag ggacgaaggc gggccagcgc cgagaggtag cggcggctgt
     4021 cgcgcgctcc ctggcgaaac gtcgccagcg cggcccggat gcgctcgatc atgatccgtt
     4081 ctccggcggg cgccaggcgt cgtgcgccga ccagcggaac agctggacca gctcccgcgc
     4141 gctgtttcca agatcgaagc gttcctcgac cgcacgcctc gcctcggcgg cgaggcgagc
     4201 ggccagggcg tcgtcggcga tcaggcggcg tatggcgtcg gcgagcgccc cggcatcgcc
     4261 gcagggcacg tgaagtcccg tggcgccgtc ctggacggcc tcggggatgc ccgagagggg
     4321 ggtggtcacg acgggcagac cgaccgccat cgcctcgagg atcacggtgg gaatgccgtc
     4381 ctgatcgccg tctgcggcga cgatgcacgg ctgggcgagc aggtgggcgc gatcgaacag
     4441 ctcgatgagc cggtcctgcg ccacggggcc gaggagctgg acggtcgatc caagtccgag
     4501 ctcgtcgatc cggcgctcga ggcgcccgcg ctccggtccg tcgccgacga tccacgcgcg
     4561 cgcccgcatt ccgcgatgaa gcaggaccga catcgcgtcg atcagcacgc cgaacccctt
     4621 cttctccacc agacgcccga cggcgagcac ggtcggagtc tcctcacgga ccatgacggg
     4681 gtctcccgga catggcgaga ccggccggcc ttgggggcgc cggaac
//
LOCUS       DASSLZ010000055         6376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_274694, whole genome shotgun sequence.
ACCESSION   DASSLZ010000055 DASSLZ010000000
VERSION     DASSLZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274694"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFM00_01920"
     CDS             <1..557
                     /locus_tag="VFM00_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7497033.1"
                     /translation="TLLHLLGGLDRPTAGTVRVAGVAMSSLDEEGRSKLRGRSVGFVF
                     QFHQLLPEFTALENAMMPGWIAGRPTPQVRKRAESLLRDLGLGSRLEHKPSELSGGEQ
                     QRVAVARALHLQPAVLLADEPTGNLDRPATMALLEILERYRQEESQAIVIATHNAEVA
                     GSAGRVLSLQDGRLQPMKVVPTRI"
     gene            605..1135
                     /locus_tag="VFM00_01925"
     CDS             605..1135
                     /locus_tag="VFM00_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006212958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HET7497034.1"
                     /translation="MQCQLCGKRPAIVHFTEIVNNKKSEYHVCEKCAEERGYSVPLLK
                     TKFSVGDLLAGMVDQTGVGEEAKVGRVQCPRCGMVYSSFRETGRLGCSECYVTFRTQL
                     RPLLRRIHGSTKHVGKSPQRDSVRTNLRHEIQRLHEEMQRAIEREEFETAANLRDQIR
                     AMEGQETPETTGEGAA"
     gene            1132..2208
                     /locus_tag="VFM00_01930"
     CDS             1132..2208
                     /locus_tag="VFM00_01930"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HET7497035.1"
                     /translation="MKLTTLARSLGRWLDGTGPSSDIVLSTRVRLARNMKDVPFTHRA
                     REEQLAMVYSSAITAVRKTPPLAHSSAFQMRDLTPLDRQFLVERHLISNDLADNGKLR
                     GLLVMPDESVAVMVNEEDHLRLQALASGFQLRSAWEAVNAIDDELAQDLDYAFSDELG
                     YLTACPTNAGTGMRASVLIHLPSLVLTKQIGKVLQGITQVGLAVRGFYGEGSQIMGNF
                     FQISNQTTLGQSERETIDSLERVTRQIIDYEQRARDELVKDARVQIEDKIWRAYGTLR
                     HSRVISSQEVINLSSAVRFGVALNMEGLTDVPTLNEMLIRTQPAHLQLFTGREMEQRE
                     RNVVRADYVRHLLEGGDDDAVRSN"
     gene            2233..4650
                     /locus_tag="VFM00_01935"
     CDS             2233..4650
                     /locus_tag="VFM00_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET7497036.1"
                     /translation="MHDKFTERVRKVMYLAREEAARLQHDYIGTEHLLLGVIREGEGI
                     AATVLNNLGLDLDAIRQAVESMVASTGGTLTIGEIPFTPRAKRVLELSVDEARQLGHN
                     YVGTEHLLLGLIREGEGVAARVLLELGVDRKKVREETLKLLGGTPSASQPEREGKVET
                     PALNQFGRDLTALARENKLDPIIGRESEIERVIQVLSRRKKNNPILIGEPGVGKTAIA
                     EGLAERIVENRVPETLKDKRIVTLDLAAVVAGTKYRGQFEERLKAVMNEIRESKDTII
                     FIDELHTIVGAGGAEGAIDASNMLKPALARGELQCIGATTLDEFRKYIEKDGALERRF
                     QPIMVDPPSVDETIQIIMGLRDKYEAHHGSKYTDDAIVAAVKLADRYINDRFLPDKAI
                     DVIDEAGACARLSMSAIPSEVRDLEKQVEEVAKEKEASIQQQEFEKAARFRDKEKELR
                     AKLREHKKNWNEIKHETEVKVDADLIAQVISRMTGIPISRIEEKESEKLLRMEEEIRK
                     SIVGQDEAVAAVSRAIRRNRAGLRDPRRPIGSFIFLGPTGVGKTELARVLARFLFDDP
                     DALIRIDMSEYMEKFSVSRLIGAPPGYVGHEEGGQLTEKVRRKPYSVVLLDEIEKAHP
                     DVFNILLQVLDDGQLTDSLRRRVDFKNTVLIMTSNLGARQIKGQRALGFGERTEDSTY
                     DNMKRTILEELKRNFNPEFLNRIDEAIVFHPLSRPQMEQITGILLQQVSERLRQSGVK
                     VTFEPSVVDLLIEKGFDAALGARPLKRAIQKLIEDPLSEHVLRGQLKEGSDVVISRAG
                     DELTFAS"
     gene            4650..>6376
                     /gene="bamA"
                     /locus_tag="VFM00_01940"
     CDS             4650..>6376
                     /gene="bamA"
                     /locus_tag="VFM00_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HET7497037.1"
                     /translation="MPDAIRAAAPRRGSRGLAAHAALPALAALLLAALTLGFGAAGAR
                     AQVANPPSASPFDTTAAPVAPAAPLSEPPAATTPPPSGPAGVIHSVRASGFANVDSTV
                     ILRTFGFKAGDAYDPDGVRAGVRRLFATGLFTDVDVQDTSTPLGVDLNVQVKERTRIQ
                     GVQFSGAKKIEEKTLKTKITVATGQLVDPGTLELDARKIQDAYAEEGYGHARVHPITR
                     PAGPGAVTVIFQVEEGEKQKVRGITVHGANTLSAEGIRKSMKSRTPAFLKSGTYKPSQ
                     VEDDETKIRLYMRSRGFRDADVDSIRTVETSDGKGVLLHVYVREGPRYRFGNVAWTGN
                     SAVPRAALDIATVIRPGTPYNESQVQKTLEQAYSLYQEQGYLFLSIDPKFADRDSVVD
                     VNFQVQEGNRSRVADLRIVGNTRTKENVIRREASVRPGDVFRRSALIRTQRDIFGLGY
                     FQDVNVDYEPTGDSADIRLTLKVQEKQTGTASAGAGYSSQTGVTGFLELGHNNLFGNG
                     QSIAVRLERGSKRSNIEFSFTEPWFRDTPLTLGADIFNTQRDLDFYDRKDVGGGIRLG
                     RPLPWPDYTR"
BASE COUNT         1173 a         2098 c         2203 g          902 t
ORIGIN      
        1 cgacgctgct gcacctgctg ggggggttgg accggcccac ggccggaacg gtgcgggtcg
       61 cgggggtcgc gatgtcctcc ctcgacgagg aggggcgctc caagcttcgg ggccggtcgg
      121 tggggttcgt ctttcaattt caccagcttc tccccgaatt caccgcgctg gagaacgcga
      181 tgatgccggg gtggatcgcc ggaaggccga cgcctcaggt gcggaagcgg gctgaaagcc
      241 ttcttcggga cctgggactg ggcagccggc tggagcacaa gccctccgag ctttcgggcg
      301 gagaacagca gcgcgtggcc gtggcgcggg ccttgcatct ccaaccggcg gtgctgcttg
      361 cggacgagcc gaccggcaat ctggaccgac cggccaccat ggcccttctg gagattctgg
      421 agcggtaccg gcaggaagaa tcccaagcca ttgtgatcgc gacgcataat gcggaagtag
      481 ccggttcggc gggacgggtg ctgagcctac aggatgggcg actccagccg atgaaagtgg
      541 tgccgacccg catctaaacg gccaggaacc gtctgcacgg agtcgtcagg agggaagagc
      601 ggccatgcag tgtcagctgt gcgggaagcg tcccgccatc gtccacttca cggaaatcgt
      661 gaacaacaag aaatccgaat accacgtctg cgagaagtgc gccgaagaac ggggctacag
      721 cgtccccctt ctgaagacga agttttccgt cggcgatctt ctcgcgggca tggtggacca
      781 gaccggcgtc ggcgaggaag ccaaggtcgg gcgcgtgcag tgcccgcgct gcggcatggt
      841 ctactcgagc ttccgggaaa cgggccggct cggctgctcc gagtgctatg tgaccttccg
      901 cacccagctg cggccgctcc tgcgccggat ccacggtagc acgaagcacg tcggcaagtc
      961 gccgcagcgc gacagcgtgc gcacgaacct gcgccacgag atccagcgcc ttcacgaaga
     1021 gatgcagcgc gcgatcgagc gggaggagtt cgagaccgcc gcgaacctcc gcgaccagat
     1081 ccgcgccatg gaggggcagg agacgcccga gacgaccggg gagggggcgg cgtgaagctg
     1141 accacgctcg cgcgatcgct gggccgctgg ctggacggga cggggccgtc gtcggacatc
     1201 gtcctctcca cgcgcgtccg gctcgcgcgc aacatgaagg acgtgccctt cacccaccgg
     1261 gcgcgcgaag agcagctggc gatggtctac tccagcgcga tcaccgccgt gcgcaagacg
     1321 ccgccgctcg cgcactcgtc ggccttccag atgcgcgatc tgacgccgct cgaccgccag
     1381 ttcctggtcg agcgccatct gatctcgaac gatctggccg acaacgggaa gctccgcggg
     1441 ctgctcgtga tgcccgacga gagcgtggcg gtcatggtga acgaagagga ccatctgcgc
     1501 ctgcaggcgc tcgcgtcggg gtttcagctc cgctcggcct gggaggcggt caacgccatc
     1561 gatgatgaat tggcgcagga cctcgactat gctttcagcg acgagctcgg ctatttgacg
     1621 gcgtgcccga cgaatgcggg caccggcatg cgcgcctcgg tgctgatcca cctgccctcg
     1681 ctcgtcctga ccaagcagat cggcaaagtg cttcaaggaa tcacgcaggt ggggctcgcc
     1741 gtgcgcggct tctacggcga gggttcgcag atcatgggga acttcttcca gatctcgaac
     1801 cagacgacgc tgggtcagag cgagcgggag acgatcgaca gtctggagcg cgtgacgcgc
     1861 cagatcatcg actatgagca gcgcgcgcgg gacgagctgg tcaaggacgc ccgcgtgcag
     1921 atcgaggaca agatctggag ggcctacggg accctgcggc acagccgcgt gatctcgtcc
     1981 caggaagtga tcaacctctc ctcggcggtg cgcttcggcg tggcgctgaa catggagggg
     2041 ctgaccgatg tgccgacact gaacgaaatg ttgatccgca cccagccggc gcacctgcag
     2101 ctcttcacgg ggcgcgagat ggagcagcgc gaacggaacg tggtccgcgc cgactacgtc
     2161 cgccacctcc tcgaaggagg cgacgacgat gcggtgcgct cgaattaggg cgaggcggga
     2221 ggctcgcagc tgatgcatga caagttcacg gaacgcgtgc gcaaggtgat gtacctcgcc
     2281 cgcgaggagg cggcccggct ccagcacgac tacatcggga ccgagcatct tctcctgggc
     2341 gtcatccgcg aaggagaggg aatcgccgcc acggtgctga acaacctggg cctcgacctg
     2401 gacgccatcc gccaggcggt cgagagcatg gtggcctcca ccggaggcac gctcacgatc
     2461 ggcgagatcc ccttcacgcc gcgggcgaag cgggtgctcg agctgtcggt ggacgaagcg
     2521 cgccagctgg gccacaacta cgtcggcacc gaacacctcc tcctggggct catccgggaa
     2581 ggggagggcg tcgccgcgcg cgtcctgctg gagctgggcg tggaccggaa gaaggtgcgc
     2641 gaggagaccc tgaagctcct gggcggcacg ccgtcggcgt cgcagccgga gcgcgagggg
     2701 aaggtcgaga cgccggcgct gaaccagttt ggccgcgacc tgaccgcgct cgcgcgggag
     2761 aacaagctgg atccgatcat cgggcgcgag tccgagatcg agcgggtgat ccaggtgctc
     2821 tcgcgccgca agaagaacaa cccgatcctg atcggggagc ccggcgtggg caagaccgcc
     2881 atcgccgagg ggctggccga gcggatcgtc gagaaccggg tcccggagac gctcaaggac
     2941 aagcggatcg ttacgttgga cctcgcggcg gtggtcgcgg gtaccaagta tcgcggccag
     3001 ttcgaggagc gcctcaaggc ggtcatgaac gagatccggg aatccaagga cacgatcatc
     3061 ttcatcgacg agctccatac catcgtcggg gcgggcggcg ccgagggcgc catcgacgcc
     3121 tcgaacatgc tgaagcccgc gctggcgcgc ggtgagctcc agtgcatcgg ggccaccacg
     3181 ctcgacgagt tccggaagta catcgagaag gacggggccc tggagcggcg cttccagccg
     3241 atcatggtcg accccccctc ggtggacgag acgatccaga tcatcatggg gctgcgcgac
     3301 aagtacgagg cgcaccacgg ctccaagtac accgacgacg ccatcgtcgc ggcggtgaag
     3361 ctggccgacc gctacatcaa cgaccgcttc ctcccggaca aggccatcga cgtgatcgac
     3421 gaggcgggcg cgtgcgcgcg cctctcgatg tcggccatcc cctccgaggt gcgcgatctc
     3481 gagaagcagg tcgaggaagt ggccaaggag aaggaagcct cgatccagca gcaggaattc
     3541 gagaaggcgg cccgcttccg cgacaaggag aaggagctcc gcgccaagct ccgcgagcac
     3601 aagaagaact ggaacgagat caagcacgag accgaggtca aggtggacgc cgacctcatc
     3661 gcgcaggtca tctcgcgcat gaccggcatc ccgatctcgc gcatcgagga aaaggaatcg
     3721 gagaagctcc tccgcatgga ggaagagatc cggaagtcga tcgtcgggca ggacgaggcc
     3781 gtggcggccg tttcccgggc catccggcgc aatcgggccg ggctccggga tccacggcgc
     3841 ccgatcggct ccttcatctt cctcggcccg acgggcgtgg ggaagacgga gctggcgcgc
     3901 gtgctggcgc ggttcctctt cgacgacccg gacgcgctca tccggatcga catgtccgag
     3961 tacatggaga agttctcggt ctcgcggctg atcggcgccc cgccggggta cgtcggccac
     4021 gaagagggcg ggcagctgac ggagaaggtg cgccggaagc cctactcggt ggtgctcctc
     4081 gacgagatcg agaaggccca tcccgacgtg ttcaacatcc tgctccaagt gctggacgac
     4141 ggacagctca ccgacagcct gcgccgccgc gtcgacttca agaacacggt gctgatcatg
     4201 acgtcgaacc tgggggcccg gcagatcaag gggcagcgcg cgctgggctt cggcgagcgc
     4261 accgaggact cgacctacga caacatgaag cggacgatcc tcgaggagct gaagcgcaac
     4321 ttcaatccgg agttcctgaa ccggatcgac gaggcgatcg tcttccaccc gctgtcgcgg
     4381 ccgcagatgg agcagatcac gggcatcctg ctgcagcagg tgtccgagcg cctgcgccag
     4441 agcggcgtca aggtgacgtt cgagccctcg gtcgtcgacc ttctcatcga gaaggggttc
     4501 gacgccgcgc tgggagcccg tcccctgaag cgggccattc agaagctgat cgaagacccc
     4561 ctctccgagc acgtcctgcg cggccagctg aaagaaggca gcgacgtggt catatcccga
     4621 gccggtgacg agctgacctt tgcgtcttga tgcctgacgc catccgagcc gcggcgcccc
     4681 gaagggggag ccgcggcctc gcggcccacg ccgcgctccc cgcgctcgcc gcgctccttc
     4741 tggcggcgct gacgctcggg ttcggggcgg cgggcgcgcg cgcccaggtc gccaacccgc
     4801 cgtcggcttc gccgttcgat accacggcgg cgcccgtggc gccggcagcg ccgctttccg
     4861 agcctcccgc ggccacgacg ccgccgccct cagggccggc cggggtcatc cactccgttc
     4921 gggcctccgg cttcgccaac gtggactcca ccgtcatcct ccggaccttc gggttcaagg
     4981 cgggcgacgc ctacgatccc gacggcgtgc gcgcgggcgt gcggcgtctc ttcgcgaccg
     5041 gcctcttcac cgacgtggac gtccaggaca cctccacccc tctgggcgtg gacctgaacg
     5101 tgcaggtcaa ggagcggacg cggatccagg gcgtacagtt cagcggcgcg aagaagatcg
     5161 aagagaagac gctcaagacc aagatcacgg tggcgacggg ccagctcgtc gatcccggca
     5221 cgctggagct cgacgcccgg aagatccagg acgcctacgc cgaggagggc tacggccacg
     5281 cgcgcgtcca tccgatcacg cgccccgccg gtcccggcgc cgtcaccgtc atcttccagg
     5341 tcgaggaggg ggagaagcag aaggtgcgcg gcatcaccgt gcacggcgcg aacacgctct
     5401 cggccgaggg aatccggaag tcgatgaaga gccggacgcc ggccttcctg aagagcggga
     5461 cctacaagcc ctcgcaggtg gaagacgacg agaccaagat ccgcctctac atgcggagcc
     5521 gcggcttccg cgacgccgac gtcgattcga tccgcacggt cgagacctcc gacggcaagg
     5581 gcgtcctgct ccacgtctac gtgcgggagg ggccgcgcta ccggttcggg aacgtcgcgt
     5641 ggaccgggaa cagcgccgtg ccgcgcgcgg cgctcgacat cgcgacggtg atccgacccg
     5701 gcaccccgta caacgagagc caggtccaga agacgctgga gcaggcctac tcgctctacc
     5761 aggagcaggg gtacctcttc ctttccatcg accccaagtt cgccgaccgc gactcggtgg
     5821 tggacgtcaa cttccaggtg caggagggga accgctcgcg cgtggccgac ctccggatcg
     5881 tggggaacac gcgcaccaag gaaaacgtca tccgccgcga agcctccgtt cgccccgggg
     5941 acgtcttccg gcgctccgca ctgatccgga cccagcggga catcttcggc ctgggctact
     6001 tccaggacgt caacgtggac tacgagccga ccggcgacag cgcggacatc cggctgaccc
     6061 tgaaggtcca ggagaagcag acgggaacgg cgagcgccgg agcggggtac tccagccaga
     6121 ccggcgtcac gggcttcctg gagctgggcc acaacaacct gttcggcaac ggccagagca
     6181 tcgcggtgcg gctggaacgg gggagcaagc ggagcaacat cgagttctcc ttcaccgagc
     6241 cctggttccg ggacaccccg ctcacgctgg gggccgacat cttcaacacg cagcgcgatc
     6301 tggacttcta cgaccggaag gacgtgggcg gggggatccg gctggggcgg ccgctgccgt
     6361 ggccggacta cacgcg
//
LOCUS       DASSLZ010000056         5442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1415360, whole genome shotgun sequence.
ACCESSION   DASSLZ010000056 DASSLZ010000000
VERSION     DASSLZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415360"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /gene="bshC"
                     /locus_tag="VFM00_01945"
     CDS             complement(<1..1466)
                     /gene="bshC"
                     /locus_tag="VFM00_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HET7497038.1"
                     /translation="MRPFYPCDFRDPAAAVAAARARDYPEARRAKTASILARQAEAWG
                     VLEPSREALARFARADSVAVVSGQQPGLFGGPLYTLYKMLTAANLARSIESATGAAAV
                     PIFWIASDDHDFEEVRATAVSDGTAEPTPLAYPAEAAPRGVSVSRIRFGPAVEALVRS
                     AESVVPPSPFRESVLARLRAAYAPGRGFSEAFARFLAPIAAELGVLLFEASDEEAKAA
                     ALPVFEREVALGGGSSAAARARGEALEKAGYHAQIARAGNELNLFWHGREREAIRVTD
                     AGAFRLAGSGQELSGSKLLALIRNRPADVSPGVLLRPLMQDHLFPTAAYVGGPAEVAY
                     WAQVYALYPLFDLEPPAVAPRAGATLLEAKVARVLDRFHLDWKALAGDPEAAVQAGLR
                     ALLPEDFGTLFERERGELEAVLERLRSAVTQFDPSLEAAVTTAGHRIERETDGLEKKL
                     MHVWKRRQEESVQQIRRAHGHLFPHGHLQERTVSLLGYA"
     gene            complement(1568..2230)
                     /locus_tag="VFM00_01950"
     CDS             complement(1568..2230)
                     /locus_tag="VFM00_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7497039.1"
                     /translation="MRIERFPVGPLDNNLYLLTPDPSRERSSSGTDAILVDPSLESLD
                     TLRAIQARGITVKRILLTHAHIDHILMAKPFRDATGAPVWLHEGDRTLYELGPVQAEQ
                     MGLPWPGTAPIDHWITDGEDCGLPGIDVKAIHTPGHSPGSVTFVTPAGLVAGDVLFRE
                     SVGRVDLPGGDWETLVRSIRGRLFAYERGTTVYPGHGPTTTIGHEMKVNPFVGASATL
                     QA"
     gene            complement(2233..3273)
                     /locus_tag="VFM00_01955"
     CDS             complement(2233..3273)
                     /locus_tag="VFM00_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7497040.1"
                     /translation="MSHMGRTSGPLNSLAGRLSPVPILQVATGFWASQALLTAVDLRV
                     FTSLFGGAKSAAELSGELGADPNALEALLDANCALGFLQRNGDRYRNTEVSNAFLVEG
                     SPGSYVDLVRFMRQPLFGVWQALRDTVKSGDSPVPAEAMDPTEVELARAFHNGAYATM
                     MRVAEILDLEFSGFSRIVELGGHSGAGSLCLARRYPQLQATILDRAPFQPLAEEFIQS
                     TKLEDRVRFRAGNPDEGCPGEEADLVLLPHILSRRSRAAIPGILQAVRASLRPGGALI
                     VTEFLLEDGKAEPREAALFRLNVVASYGSKNAGGLTRSDLVGLLSDAGFTDVDMVGLP
                     MFGITAITATRG"
     gene            complement(3374..4495)
                     /gene="add"
                     /locus_tag="VFM00_01960"
     CDS             complement(3374..4495)
                     /gene="add"
                     /locus_tag="VFM00_01960"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET7497041.1"
                     /translation="MRRSTPRRRTSPWGSAPRRRELLRALPKTDLHVHLDGSLRPATL
                     LELAAAQGAKLPFRTPRELSAAFARLASGVSLPEYLKAFDWTLAVLQDRASLERVAFE
                     LAEDAFMENVRYMEVRYCPALHTRKRLTMEETVEAVRAGLRRAERATGIRTGTIVCGI
                     RHLSPRLSVRLADLASAYYGKGVVGFDLAGAEKDYPAKSHRRAFDHVKKRHVNITVHA
                     GEAFGAASIDQALRYCGAHRIGHGTRLREDPDVLAFVRDHRIPLEMCLVSNVQTGAVR
                     SLATHPFGRYLKAGLRVTVNTDNRLVSSTTMTDELERAVSTFRLTPADVRYVLINGFK
                     SAFLPYEEKGALLRHALRDMDRILEEARSAEGEPSRDLL"
     gene            complement(4452..>5442)
                     /locus_tag="VFM00_01965"
     CDS             complement(4452..>5442)
                     /locus_tag="VFM00_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7497042.1"
                     /translation="HNVKTICTLCSKGCNTVAGFHPRRNKILRVTARENMDVNQWWIC
                     DRGRGQFHSVHDVVRIATPLYRSRTKGAAARPVAWADAIAQVAGAIKDAIARQGADSV
                     GVVSSAELSNEELFLAGRIFGPDGLGLSNFDFPERPQPPVVYPKFTIEGDKNPNTRGA
                     RLLGGTAGKRGRSVKEILTAAAEGRIGVLLFLRGGAPETFGDAALVARALDRAGLVVV
                     MDFVASAVADLADWVLPGVSHFEKDGTYVNSQGRVQRARKVFTLRGDTREDWRILQDL
                     GSALGAIHETDPSAEMIFARIARTVPAFTGLSYSALGDRGAPLDTEAANVAVG"
BASE COUNT          811 a         1875 c         1988 g          768 t
ORIGIN      
        1 gcgtagccca ggagcgagac cgtgcgttct tggaggtgcc cgtgcggaaa cagatgcccg
       61 tgcgcgcggc ggatctgctg caccgactct tcctggcgcc gcttccagac gtgcatcagc
      121 ttcttctcca gcccgtcggt ctcccgctcg atccggtgcc cggccgtggt cacggccgcc
      181 tcgagcgagg ggtcgaactg ggtgaccgcc gagcgcagcc gctcgagcac cgcctccagc
      241 tcgccccgct cccgctcgaa gagcgtaccg aagtcttcag gcaggagcgc gcggagcccc
      301 gcctgcacgg ccgcctcggg atccccggcg agcgccttcc agtcgaggtg gaagcggtcg
      361 agcacgcgcg ccactttcgc ctcgagcagc gtcgcccccg cgcgcggcgc caccgccggc
      421 ggctccaggt cgaacagcgg atagagcgcg tagacctgcg cccagtaggc cacctccgcc
      481 ggaccgccga cgtaggcggc ggtcggaaag agatggtcct gcatgagcgg gcggagcagc
      541 acgcccgggc tcacgtcggc cgggcggttc cgaatcaggg cgaggagctt cgagcccgag
      601 agctcctgcc cgctccccgc cagacgaaac gctcccgcgt ccgtcacgcg gatcgcctcc
      661 cgctcgcgcc cgtgccagaa gaggttgagc tcgtttccgg cgcgggcgat ctgcgcgtgg
      721 tagccggcct tctccagcgc ctcgccgcgc gccctcgccg cggccgagct tccgcccccc
      781 agcgccacct cgcgctcgaa gactggaagc gccgccgcct tcgcctcctc gtcagaggcc
      841 tcgaacagca gcaccccgag ctccgcggcg atcggcgcca ggaagcgcgc gaaggcctcg
      901 gagaagccgc gccccggcgc gtacgccgcg cgcaggcgcg cgagcaccga ctcgcgaaag
      961 ggcgaggggg gcacgaccga ctccgccgag cgcaccagcg cctccaccgc ggggccgaac
     1021 cggatgcgcg acaccgagac gccgcgcggc gccgcttcgg ccgggtaggc gagcggcgtc
     1081 ggctcggccg tgccgtcgct caccgcggtg gcgcggacct cctcgaagtc gtgatcgtcg
     1141 gacgcgatcc agaatatggg tacggcggcc gcgccggtcg cggactcgat ggagcgggcg
     1201 aggttcgccg ccgtcagcat cttgtagagc gtgtagagcg gcccgccgaa cagccccggc
     1261 tgctgccccg agaccaccgc gaccgaatcg gcgcgggcga agcgggccag cgcctcgcgc
     1321 gagggctcga ggacgcccca cgcctcggcc tggcgcgcca ggatcgaagc ggtcttcgcg
     1381 cggcgcgcct ccggatagtc gcgggcgcgc gcggcggcca cggcggccgc cggatcgcgg
     1441 aagtcgcagg gatagaacgg gcgcacccgc gcgaaatcgt tcaggaaatc gcggtagagg
     1501 gcgctcggaa ggagcagccg ttcgaggtcg ctcgtcgttg gctccgcggg cggccccgtc
     1561 gtctacgcta cgcctgcagg gttgccgacg cgccgacgaa cgggttgacc ttcatctcgt
     1621 ggccgatggt ggtggtgggg ccgtgccccg ggtagacggt ggtgccgcgt tcgtacgcga
     1681 acaggcgtcc ccggatggag cggaccagcg tctcccagtc gccgcccggc agatccacgc
     1741 gtcccaccga ttcgcggaag agcacgtccc ccgccaccag ccccgcgggg gtgacgaagg
     1801 tcacgctccc gggcgagtgc ccgggcgtgt ggatcgcctt cacgtcgatg ccgggaaggc
     1861 cgcagtcctc gccatcggtg atccagtggt cgatcggcgc ggtgccgggc cacgggagtc
     1921 ccatctgctc cgcctgaacg gggcccagct cgtagagcgt gcggtcccct tcgtgcagcc
     1981 agacgggcgc gccggtcgcg tcgcggaacg gcttcgccat gaggatgtgg tcgatgtggg
     2041 cgtgcgtgag caggatccgc ttcaccgtga tcccgcgcgc ctggatggcg cgcagcgtgt
     2101 ccaggctctc gagcgacgga tccacgagga tcgcatccgt gccggagctg gagcgctctc
     2161 gcgacggatc gggtgtgagc aggtagaggt tgttgtcgag cggcccgacg gggaagcgct
     2221 cgatgcgcac gatcagcccc gcgtggcggt gatcgccgtg atcccgaaca tgggcaggcc
     2281 caccatgtcg acgtcggtga atccggcgtc gctcaagaga ccgaccagat cggaacgggt
     2341 gagccccccg gcgttcttgg agccgtagct cgccaccacg ttcagccgga agagcgccgc
     2401 ctcgcgaggt tcggccttgc cgtcctcgag gaggaactcg gtgacgatca aagcaccgcc
     2461 gggacgcagc gatgcgcgca cggcctggag gatgccggga atggcggcgc ggctgcggcg
     2521 cgagaggatg tgcggcagga gcacgaggtc ggcctcctcg cccgggcagc cctcgtcggg
     2581 gttgccggcg cggaagcgga cccggtcctc cagcttcgtc gactggatga actcttcggc
     2641 gagcggctgg aacggcgccc ggtccaggat cgtggcctgg agctgcgggt agcggcgcgc
     2701 gagacagagc gagcccgcgc cgctgtggcc gcccagctcg acgatccgcg agaagccgct
     2761 gaactcgagg tccaggatct cggccacgcg catcatggtg gcgtaggcgc cgttgtggaa
     2821 cgcgcgcgcc agctcgacct ccgtcgggtc catcgcttcg gcgggaacgg gcgaatcgcc
     2881 gctcttcacc gtgtcgcgga gcgcctgcca gaccccgaag agcggctgac gcatgaagcg
     2941 gacgaggtcg acgtaggaac cgggcgagcc ctcgaccagg aaggcgttcg acacctcggt
     3001 gttccggtag cggtcgccgt ttcgctgcag gaaccccagg gcgcagttgg cgtcgagaag
     3061 ggcttcgagg gcgttggggt cggcgccgag ctcacctgac aattcggcag ccgacttggc
     3121 cccgccgaac agcgaggtga acacccgaag gtccacggcg gtcaggaggg cctggctcgc
     3181 ccagaaaccc gtggcaactt ggaggatggg gaccgggctc agacgcccgg caagggagtt
     3241 gagcggaccg gaggtgcgtc ccatgtggct catctgtggg tcctcacaag gatcatctta
     3301 tgctcctcga agcggacgat caagcgggca cggtctcgcg ccagctgcgc tcggttccat
     3361 tagatgcgcc ttctcacaga aggtcgcgcg agggttcgcc ctcggccgag cgcgcctctt
     3421 ccagaatccg atccatgtcg cgaagagcgt gccggagcag cgctcccttc tcttcatacg
     3481 gcaggaacgc cgatttgaat ccattgatca gcacgtagcg gacgtcggcg ggcgtgagcc
     3541 ggaaggtgct gaccgcgcgc tcgagctcgt ccgtcatggt cgtggaggag acgagccggt
     3601 tgtcggtgtt caccgtgacc cggagccccg ccttgagata gcggccgaag gggtgggttg
     3661 ccaggctccg gaccgcgccg gtctggacgt tcgagaccag gcacatctcc agcgggatcc
     3721 ggtggtcgcg gacgaaggcg aggacgtcgg gatcctcgcg gagacgggtg ccgtggccga
     3781 tccggtgggc cccgcagtag cggagcgcct ggtcgatgct cgccgcgccg aaggcttccc
     3841 cggcgtggac ggtaatattg acgtgccgtt tcttcacatg gtcgaacgcg cggcggtggg
     3901 atttggccgg atagtccttc tccgccccgg cgaggtcgaa gccgacgacc cccttgccgt
     3961 agtaggccga ggccaggtcg gccaggcgca ccgagagccg ggggctcagg tgccggatcc
     4021 cgcagacgat ggtcccggtc cggatcccgg tcgcccgctc ggcgcggcga agacccgccc
     4081 gaaccgcctc caccgtctcc tccatggtca gccgcttccg ggtgtggagc gccgggcagt
     4141 agcgcacttc catgtaccgg acgttctcca tgaacgcgtc ctcggccagc tcgaacgcga
     4201 cgcgctcgag ggaggcgcgg tcttgaagta ccgccagggt ccagtcgaac gccttcaggt
     4261 actcggggag gctgactccg gaggccaggc gggcgaaggc ggccgagagc tcgcgcggcg
     4321 tgcggaacgg gagcttggct ccctgcgcgg ccgccagctc gaggagcgtg gccgggcgca
     4381 gcgagccgtc gaggtggacg tgaaggtcgg tcttggggag cgcgcgaagc agctcgcggc
     4441 gccgcggggc gctaccccac ggcgacgttc gccgcctcgg tgtcgagcgg cgcaccccgg
     4501 tcccccagag ccgaatacga gaggcccgtg aacgcgggca cggtgcgggc gattcgggcg
     4561 aagatcatct cggccgaggg atcggtctcg tggatcgcgc ccagcgcgga tcccaaatct
     4621 tgaaggatgc gccagtcctc gcgggtgtcg ccgcgcagcg tgaacacctt ccgggcgcgc
     4681 tgcacgcgcc cctgtgaatt gacgtaggtg ccgtccttct cgaaatggga gacgccgggc
     4741 agcacccagt cggcgaggtc ggcgacggcc gaggccacga aatccatcac gaccacgagg
     4801 cccgcccggt ccagcgcgcg cgccacgagg gccgcgtcgc cgaaggtctc gggggcgccg
     4861 ccgcgcagga agaggagcac gccgatccgc ccctcggccg cggcggtgag gatctccttc
     4921 accgagcgcc cgcgcttccc cgccgtgccg ccgagcaggc gcgcgccgcg cgtgttgggg
     4981 tttttgtccc cttcgatcgt gaatttggga tagaccaccg gcggctgcgg ccgctcgggg
     5041 aaatcgaagt tggagaggcc gagtccgtcc gggccgaaga tccgcccggc caggaacagc
     5101 tcctcgttcg agagctcggc cgacgagacg acgccgaccg aatcggcgcc ctggcgcgcg
     5161 atcgcgtcct tgatggcgcc cgcgacctgc gcgatcgcgt cggcccaggc gaccggccgc
     5221 gccgcggcgc ccttggtccg cgagcggtag agcggcgtgg cgatccgcac gacgtcgtgg
     5281 acgctgtgga actgcccgcg gccgcggtcg cagatccacc actggttcac gtccatgttc
     5341 tcgcgcgccg tgacgcggag gatcttgttc cggcgcggat ggaagccggc gacggtgttg
     5401 caccccttgg agcagagggt gcagatcgtc ttgacgttgt gc
//
LOCUS       DASSLZ010000057         9487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_907088, whole genome shotgun sequence.
ACCESSION   DASSLZ010000057 DASSLZ010000000
VERSION     DASSLZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907088"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFM00_01970"
     CDS             <1..319
                     /locus_tag="VFM00_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HET7497043.1"
                     /translation="YRFEAVHRHAGEGEEADASGEVLAPMTGRVAAVEARPGATVREG
                     DLLLTIEAMKMEFKVAAPASGIVREVACAPGDRVELGQVLARIAPEAPAGAAAAPPGK
                     EKP"
     gene            316..1215
                     /locus_tag="VFM00_01975"
     CDS             316..1215
                     /locus_tag="VFM00_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HET7497044.1"
                     /translation="MNVRIVEVGPRDGLQNESRPIPTESKIAFIDALSEAGYDEIEAT
                     AFVAPDRVPQLKDAEEVLRGIRRREGVLYAALVPNERGLDRALAARVGKIAVFTAASE
                     TFNRKNINATISESIERFRPVVARAKDAKLPVRGYVSTAFWCPYEGKIEPQAVVLVVK
                     QLIDLGVEEISIGDTIGKAVPAEVHALLDRLLDAAPQDAVAMHFHDTYGTAVANALAA
                     YERGITIFDASAGGVGGCPFAPGASGNVATEDLLWALTRSGASVSLDPAKLRRASDVL
                     ASALGRPLRSRVREATQGSSPGR"
     gene            complement(1238..2350)
                     /locus_tag="VFM00_01980"
                     /pseudo
     CDS             complement(1238..2350)
                     /locus_tag="VFM00_01980"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
     gene            complement(2370..3614)
                     /locus_tag="VFM00_01985"
     CDS             complement(2370..3614)
                     /locus_tag="VFM00_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7497045.1"
                     /translation="MPHESFAPLLLAVAVILAAATAGGWLAERIRQPSVLGELVAGIV
                     LGNLGLAGVHALEPFKANTSLEALAELGVIVLLFDVGLESTIGDMMKVGRSALLVALL
                     GVVTPFFLGWGAGAWLLPGASTYAHVFLGAVLTATSVGITARVLRDLHQTKSEEAKII
                     LGAAVIDDVLGLVILAVVTGIIAAAASGAGSISVIGVGWIAAKALLFLVLAIVLGSLI
                     ARHALRFFARSRLPGTLLTGGLVVCFVLSYLAAAVGLAPIVGAFAAGLILEPAFFAAF
                     EETRRGQTLRSLIHPISTILVPVFFVRTGMLVDLRSFANPAILGIAAALTVAAWIGKQ
                     ACMLGVPRRGLNRLAIGFGMVPRGEVGLIFANIGTGLVLAGERIITPPLYAAVVIMVI
                     VTTLVTPPALQWSFRRRAASAH"
     gene            3690..4301
                     /locus_tag="VFM00_01990"
     CDS             3690..4301
                     /locus_tag="VFM00_01990"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497046.1"
                     /translation="MIARKSLSFVRLAPLAAMLLIGGPLAASPALAFGTAPSSKPPKV
                     STPASPEDSAAMAAKTRATAEALYKKAWEDSEAGKADLKKNKPKDANKKFAKALKRYD
                     EATKLDPTYFEAWNMVGFCSRKTGDLKRSFAAYDKALQLKPDYDEAHEYLGEAYLMSG
                     DIAKAKEQLAWLKEKQSPEAGELEEAIEAAGKGQPADQAGKGW"
     gene            complement(4377..5195)
                     /locus_tag="VFM00_01995"
     CDS             complement(4377..5195)
                     /locus_tag="VFM00_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HET7497047.1"
                     /translation="MNGSRSRGAIRVRPGRPVRLSAIDPDSTGGFEGKDAARVSLDKD
                     LDRLRERQRLLYADGRHALLVVLQAMDTGGKDGTIRHVLSGLNPVACVVTSFKVPSAE
                     ELAHDFLWRIHKAVPPHGVIGVFNRSHYEDVLVVRVHELVPPAVWKARYEQINQFERI
                     LSENGVRIVKIFLHISKGEQKRRLQQRLDNPEKNWKFSEADLAERKLWGGYMAAYEDA
                     INRCSTDVAPWYVVPANVKWARDAMVGRLLRDTMDSMSLRRPKVSRKIAKQKIR"
     gene            complement(5295..7457)
                     /locus_tag="VFM00_02000"
     CDS             complement(5295..7457)
                     /locus_tag="VFM00_02000"
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HET7497048.1"
                     /translation="MKRLLSLFALAGLALLLTAPLALAAGEPDVGQGRWATAENGRMA
                     DNGEIVYRCGTADRGNKAPDQAQIDRWIQENRVAAGGVIPVNFHVITNGSTGNVTDAQ
                     IAAQIQVLNRNYAGLDYNGNPVSGAKNTGYTFVLASTTRTSNRKWFTMTPGSRSEAQA
                     KAALVVNPTGSLNLYTASPGQNLLGWSTFPWNLASNPTMDGVVIHYASLPGGSLAPYN
                     LGGTATHEVGHWVGLYHTFQGGCDGGNCTGGGDQVCDTPGEATATSGCPSGKDTCPEA
                     GLDPIHNYMDYSTDACYNQFTPGQDTRADAMMANYRPLIGSARLANAELPVTDFRATE
                     AHGPRSADNGDVVYRCATDAKGANMKAPKKSDVDLYIQQGRVTAGGVIPVYVHVITNG
                     STGNVTDAQIAAQIQVLNRNYAGLDYNGNPVSGAVNTGYTFVLAGTDRTSNRKWFTMT
                     PGSRNEAQAKAALARNWTSALNLYTASPGQNLLGWSTFPWDLSANGNQDGVVIHYASL
                     PGGTLSPYNLGGTASHEVGHWVGLYHTFQGGCDGGNCSGAGDLVCDTPGEASATSGCP
                     SGKDTCPDAGLDPIHNYMDYSTDACYNNFTAGQDSRADFMMSTYRPLIGSARLANAAG
                     SIQNARDLNGGLVRFAARPNPFNPRTKIEFGLAREGRATVRVFDIRGRLVSTVVDKVL
                     AAGNHSFDFDAAKLSSGVYFMRLQADGQSQVRRLSLLK"
     gene            7863..>9487
                     /locus_tag="VFM00_02005"
     CDS             7863..>9487
                     /locus_tag="VFM00_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7497049.1"
                     /translation="MIGTLLGRYRILEPLGEGGMGRVFLAEDPALGRKVAVKVLPPEF
                     SQDKDRRERLLHEARAASALNHPNILTVHDLGEADGALYVAMERIEGCTLRQWAQGRA
                     RTPSEVLRLLRQALAALGVAHQAGLVHRDLKPENIMVRTDGLLKILDFGLARSVTPGV
                     AARTATLPGTILGTAPYMSPEQVLGQPAGPASDLFSLGSILYELLTGQHPFAANSAVE
                     TMHRILHESPEAPSRVNRALTADFDFVLAKALSKDPTRRHASAHDLDIDLETLECGCG
                     TAATVDDAGAGPRTLAVLPFKNIGGDPELSYLGVGLADAVITRLSSSPDLIVRATSSI
                     APYHNQPVDPRRVGVELDASTVLDASFQRFGERFRATARLVETASGRSLWAGKVDLKF
                     DDIFEVQDQVAHGIAEAMTARLQRGAATVGPAHAPSPQAYALLLKAQEAWRDSSEDGF
                     RKGIALAEEAVRIDPAFADAWAFLGSYYHGLTDSGYDPDPTWFAKADHALGQALSLDP
                     ENQQALFAKAALRLTRGAKRDAYASLIAVGRKAP"
BASE COUNT         1484 a         3216 c         3327 g         1460 t
ORIGIN      
        1 ctaccgcttc gaggcggtgc accgccacgc cggcgagggc gaggaggcgg acgcctcggg
       61 cgaggtgctc gcaccgatga ccggccgcgt ggccgcggtc gaagcgcggc ccggcgcgac
      121 cgtgcgggag ggggatctcc tgctcacgat cgaggcgatg aagatggagt tcaaggtggc
      181 ggcgcccgcg tcgggcatcg tccgcgaggt ggcgtgcgct cccggcgacc gggttgagct
      241 gggccaggtg ctcgcgcgga tcgctcccga agcgccggcc ggtgccgccg ccgcgccgcc
      301 gggcaaggag aagccgtgaa cgtacggatc gtcgaggtgg gcccgcgcga cggactgcag
      361 aacgagagcc gccccatccc gaccgagtcg aagatcgcct tcatcgacgc gctctcggag
      421 gcgggatacg acgagatcga ggccacggcg ttcgtcgctc ccgaccgggt cccccagctc
      481 aaggacgcgg aggaggtgct ccggggcatc cggcgccgcg aaggggtcct ctatgcggcg
      541 ctcgtgccca atgagcgcgg gctggaccgc gcgctcgcgg cccgtgtggg gaagatcgcg
      601 gtgttcaccg ccgcttcgga gacgttcaac cggaagaaca tcaacgccac catctccgag
      661 tcgatcgagc gcttccggcc ggtcgtcgcg cgcgccaagg acgcgaagct gccggttcgg
      721 ggctacgtct ccacggcgtt ctggtgtccc tacgaaggaa agatcgagcc ccaggccgtg
      781 gtcttggtcg tgaaacagct catcgacctt ggggtggagg agatctcgat cggcgacacg
      841 atcggcaagg ccgtgcccgc cgaggtgcac gccctcctcg accggctcct cgacgccgcg
      901 ccccaggacg cggtcgcgat gcacttccac gacacctacg gcaccgccgt ggcgaacgcg
      961 ctcgcggcct acgagcgcgg catcaccatt ttcgacgcct cggccggagg cgtgggcggt
     1021 tgtccctttg cccccggcgc ttcggggaac gtggcgaccg aggatctcct gtgggcgctc
     1081 acgcgctccg gcgcttcggt ttcgctcgac cccgcgaagc tgcggcgcgc ttcggacgtg
     1141 ctggcttccg ccctcgggcg gccgctccgt tcgcgcgtcc gcgaggcgac gcaagggtct
     1201 tctcccggac ggtgatcttg agctcgtgct tgccgatctc cttgaggaac gcctcggccg
     1261 ggatcgcccg ctccatcgcg gccacgcccc ggaagggaac ctcgccgcgc gccatcatca
     1321 gcgcggccgc cgtcgtcgga taagccgtcg tccgcatcat cgcgctcacg ccgcgcttca
     1381 tgtccatcgc atccagcatc tcgaaggaga tctcgcgccg cttgccgtcc ttcacgccgt
     1441 tcaccgtcac gcgcatcacg accacgtccg gctcgttcgg gaacgtgagg agcggctcca
     1501 cgacggccgc gaagagcgac cgcggcgaga ccgggacccc ttccacgggg accggcgtcg
     1561 tctcgaggag cccgaggtcg cggagcagcc ggatccgcgc cgcgtggccg gggtagcgca
     1621 ccgtcttgta gtccagcgtc ttcacctttc cctcgaacgt ccagggcatg gtggaggtgc
     1681 cgcccagcgt cacgaaggcc tcgcacgtgc cgaccggctt cggaaacgtg agcgtctcct
     1741 cctcggtcag cgtctcgacc tcgacccgct tgccgccgcg gagcgcggtg gcgtagccca
     1801 ggtattcgtt caggagcccg tacatcgaga aaaccatcat gtagtcgagc ggagggcggg
     1861 ggcgaagcgg caggccgccg acccggatgt ggacctcgtc ggcgcggtcc agccgcccga
     1921 tgccggccgt ggccagcacg ttcgccatgc cgggggccag gccgcagtcg gggacgatcg
     1981 tgacgtccgc gtcgcgcgcc cgctcgtcca gcgcttcctg ctcccgcacc aggtcggtgt
     2041 tcccgcccag gtcgcagaag gagacccccg cctcgatcgc cgcgcgcgtg agcgtgaggt
     2101 tcaggaagta ggggacggcc gagatcacca cgtcgtagcc gcgcagcttg tcggacagcc
     2161 gcggctcgtt cgccacgtcg agcacctcgg cccgcacggc cgcgcgggag cccgagagcc
     2221 gctccgccgc cttgcgcgcg cggtttccgt ccacgtccgc gatcacgact tcctccacgt
     2281 ccggcgcccg cgccaagtcc agcgccgcgg cgaatccctg atatcccgct ccgagcacga
     2341 ggaatctcat acgcttcggt tctccttcct caatgagccg acgccgcccg acgccggaag
     2401 ctccactgga gcgccggcgg cgtgacgagc gtcgtgacga tgaccatgat gacgaccgcc
     2461 gcgtagagcg gcggcgtgat gatgcgctct ccggccagca ccaggccggt tccgatattc
     2521 gcgaagatga gcccgacctc gccgcgcgga accatcccga atccaatcgc gagccggttc
     2581 aacccccgcc gcggcacgcc cagcatgcac gcctgcttgc cgatccaggc cgccaccgtg
     2641 agcgcggccg cgatgccgag gatggcgggg ttggcgaagg agcgcaggtc cacgagcatc
     2701 ccggtccgga cgaagaagac cggcaccagg atggtggaga tcgggtggat cagggagcgg
     2761 agagtctggc cgcgccgggt ctcctcgaac gcggcgaaga acgcgggctc gagaatgagt
     2821 cccgccgcga aggcgcccac gatcggcgcg agcccgacgg cggccgccag gtaggagagg
     2881 acgaaacaga ccacgagtcc ccccgtgagc agcgttcccg gcaggcgcga gcgcgcgaag
     2941 aagcggagcg cgtgccgggc gatgagcgac ccgagcacga tggccagcac gaggaagagg
     3001 agcgccttgg ccgcgatcca gcccacgccg atcacggaga tcgatcccgc tcccgaggcc
     3061 gccgcggcga tgatcccggt caccacggcc aggatcacca gccccagcac gtcgtcgatc
     3121 acggccgctc ccaggatgat cttcgcctcc tcgctcttcg tctggtgcag gtcccgcagc
     3181 acccgcgccg tgatgccgac gctggttgcg gtcagcaccg cgcccaggaa gacgtgggcg
     3241 taggtcgaag cgccggggag gagccacgcg cccgcgcccc agcccaggaa gaagggcgtg
     3301 accacgccga gcagcgcgac caggagcgcg gaacgcccca ccttcatcat gtcgccgatc
     3361 gtggattcca gccccacgtc gaagaggagc acgatcaccc ccagctcggc gagggcctcg
     3421 agcgacgtgt tcgccttgaa cggctcgagc gcgtgaacgc ccgcgagtcc caggtttccc
     3481 agcacgatgc cggcgaccag ctctcccaga acggacggct gccggatgcg ctcggccagc
     3541 caccctcccg ccgtcgcggc ggccaggatg accgccaccg cgagaaggag cggggcgaac
     3601 gactcatggg gcataccggc agaataccgg ctcaccgggc gatttggtag gatcggggca
     3661 accatttcca ccacgacccg gaggatctca tgatagctcg gaagtccttg tcgttcgtgc
     3721 ggttggcgcc cttggccgcg atgctcctga tcggcggccc gctcgcggcc tcaccggccc
     3781 tggccttcgg caccgctccc tcgtccaagc cgcccaaggt ctcgacgccc gcttctccag
     3841 aagattcggc ggccatggcg gcgaagacgc gggccaccgc cgaggccctc tacaagaagg
     3901 cctgggagga ctcggaagcg ggcaaggcgg atctcaagaa gaacaagccc aaggacgcga
     3961 acaagaagtt cgcgaaggcg ctgaagcgtt acgacgaggc gaccaagctc gatcccacct
     4021 acttcgaggc ctggaacatg gtcggcttct gctcgcggaa gacgggcgac ctgaagcgct
     4081 ccttcgccgc ctacgacaag gcgctccagc tcaagcccga ctacgacgag gcgcacgagt
     4141 acctgggcga ggcgtatctc atgtcgggcg acatcgcgaa agcgaaggag cagctggcct
     4201 ggctgaagga gaagcagagc cccgaggccg gcgagctgga agaggcgatc gaagcggcgg
     4261 ggaaggggca gcccgcggat caagcgggaa agggttggtg acgacgaagg tcgatcacgg
     4321 ttctgagagc ggctccccgc gcgcggcgcg agggggccgt tctcgtttgg gaacggtcag
     4381 cggatcttct gcttcgcgat cttgcgcgag accttgggcc ggcgaaggct cattgagtcc
     4441 atggtgtcgc gaagcagccg tccgaccatg gcgtcccgcg cccacttcac gttcgccggc
     4501 accacgtacc agggagcgac gtcggtcgag cagcggttga tcgcgtcttc gtacgccgcc
     4561 atgtagcccc cccagagctt ccgctctgcg aggtcggctt cggagaactt ccagttcttc
     4621 tccgggttgt ccaggcgctg ctgcagccgg cgcttctgct cccccttgga gatgtggagg
     4681 aagatcttca cgatccgcac gccgttctcg ctcaggatcc gctcgaactg gttgatctgc
     4741 tcgtagcgcg ccttccacac ggccggggga accagctcgt ggacccggac gaccagcacg
     4801 tcctcgtagt gggagcggtt gaagacgccg atcacgccgt gcggcgggac cgccttgtgg
     4861 atgcgccaga ggaaatcgtg cgccagctct tcggcgctcg gcaccttgaa cgaggtgacg
     4921 acgcaggcga cggggttgag gccgctcagc acatggcgga tcgtgccgtc cttcccgccg
     4981 gtgtccatcg cctggagcac gacgagcagg gcatgccgcc cgtcggcgta gagcagacgc
     5041 tgccgctcgc gcaggcgatc gaggtccttg tcgagggaga cgcgcgcggc gtccttgccc
     5101 tcgaagccgc cggtggaatc ggggtcgatc gccgagagcc ggacgggacg tccgggacgg
     5161 acgcggatcg ctccgcggga gcgggagccg ttcatccacc tagggtagca cggggatgaa
     5221 ggaaaacggg gccggcgatg gcgccggccc cgctccgcgg atccgccagg gatccgatgg
     5281 tggttgttgt tgggttactt gagcagcgac agacggcgca cctggctctg gccgtcggcc
     5341 tgcagccgca tgaagtacac gccgctggag agtttcgcgg cgtcgaagtc gaagctgtga
     5401 ttgcccgccg cgagcacctt gtccaccacc gtcgagacca gccggccccg gatgtcgaac
     5461 acccgcaccg tggcccgtcc ttcacgcgcc agaccgaact cgatcttggt gcgtggattg
     5521 aaggggttgg gccgcgccgc gaagcggacg aggccgccat tcaggtcgcg ggcgttctgg
     5581 atcgagccgg ccgcgttcgc gaggcgcgcc gagccgatga gcggccggta cgtcgacatc
     5641 atgaagtccg cgcgggagtc ctggcccgcc gtgaagttgt tgtaacaggc gtcggtcgag
     5701 taatccatgt agttgtggat cggatcgagg cccgcgtcgg ggcaggtgtc cttgccgctc
     5761 gggcagcccg aggtggcgct ggcttcaccc ggcgtgtcac agacgagatc gccggcgccc
     5821 gagcagttgc cgccgtcgca gccgccctgg aacgtgtgat agaggccgac ccagtgcccg
     5881 acctcatgcg aggcggtgcc gcccaggttg tagggggaca gggtgccgcc cggcagcgag
     5941 gcgtaatgga tcacgacccc gtcctggttc ccgttcgcgg agagatccca cgggaaggtg
     6001 gaccagccga gcaggttctg gcccggagag gcggtgtaca ggttgagcgc gctcgtccag
     6061 ttcctcgcga gggccgcctt ggcctgcgcc tcgttgcggg atcccggcgt catcgtgaac
     6121 cacttgcggt tgctcgtgcg atccgtgccc gcgaggacga acgtgtagcc cgtgttcacc
     6181 gcgcccgaga ccgggttgcc gttgtaatcc aagccggcgt agttgcggtt gagcacctgg
     6241 atctgggccg cgatctgcgc gtcggtgacg ttgccggtgg agccgttcgt gatgacgtgg
     6301 acgtagaccg gaatcacccc gcccgccgtc acgcgaccct gctggatata gagatcgacg
     6361 tccgacttct tcggcgcctt catgttcgcg cccttggcgt cggtggcgca gcgatagacc
     6421 acgtcgccgt tgtcggcgct gcgcgggccg tgcgcttcgg tggcgcggaa gtcggtgacc
     6481 ggaagctcgg cgttggcgag acgcgcggag ccgatgagcg gccggtagtt ggccatcatg
     6541 gcgtcggcgc gcgtgtcctg gccgggcgtg aactggttgt agcaggcgtc ggtcgagtaa
     6601 tccatgtagt tgtggatcgg atcgagcccc gcctcggggc aggtgtcctt gccgctcggg
     6661 catcctgacg tcgcggtcgc ttcgcccggg gtgtcgcaga cctgatcgcc gccgcccgtg
     6721 cagttcccgc cgtcacagcc gccctggaag gtatggtaga ggccgaccca gtggccgacc
     6781 tcgtgggtcg cggtcccgcc caggttgtag ggcgcgagcg atccgcccgg cagcgacgcg
     6841 tagtggatga cgacgccgtc catggtggga ttggaggcga gattccacgg gaacgtggac
     6901 cagccgagca ggttctggcc gggggacgcg gtgtagaggt tgagcgatcc cgtggggttc
     6961 acgaccagcg ccgccttggc ctgggcctcg ctgcgcgagc cgggcgtcat ggtgaaccac
     7021 ttccggttgc tcgtgcgcgt ggtgctcgcc agcacgaacg tgtagccggt gttcttggct
     7081 ccggagaccg ggttgccgtt gtagtccagc cccgcgtagt tccggttcag cacctggatc
     7141 tgcgccgcga tctgcgcgtc ggtgacgttg ccggtcgagc cgttcgtgat gacatggaag
     7201 ttcaccggga tcacgccgcc cgccgcgacg cggttctcct ggatccagcg gtcgatctgc
     7261 gcctggtcgg gcgccttgtt gccgcgatcg gcggtgccgc agcggtacac gatctcgccg
     7321 ttgtcggcca tgcggccgtt ttcggcggtg gcccaccggc cctggcccac gtccggttcc
     7381 cctgccgcaa gtgcgagggg agccgtcagg agaagggcga gaccggcgag tgcgaagagg
     7441 gacagaagac gcttcatgct gagacctcct ggatgcttcg ttcgggttgg gacgtcaccg
     7501 gcacgaacga gttcgaccac gcgtgaagtc ggaggcgagc ggccgcgtgg gacgggggaa
     7561 cagccagggg aggcgcacgg gtgggcgatg tcccggccgc gggtggggat ggccggaatc
     7621 gtggcaaaac ggagagccct cgttcggtcg tgagccaacg agtctcgtgc tcctgtgttt
     7681 gcctgggtgg atggaaggag gatgagctcg gcttatcgcc ccccgaaccg ccgggctccc
     7741 ttccagagcg ctaccgtacc caaatcacgt ccgttgcgca agcacgcggt gacgccggaa
     7801 cgtcgatttc gggtgttttc acccgctccg gaactgccgc gcaaccgtgc tatcctccgg
     7861 cgatgatcgg caccctgctc ggccgctacc gcatcctcga gccgctcggc gagggcggga
     7921 tgggacgcgt gttcctcgcc gaggatcccg cgctcgggcg gaaggtcgcc gtgaaagtgc
     7981 tgccgccgga gttttcgcaa gacaaggacc ggcgcgagcg ccttctgcac gaagcgcgcg
     8041 cggcgtccgc gctcaatcac ccgaacatcc tcaccgtcca tgatctcggc gaagcggacg
     8101 gcgccttgta tgtcgcgatg gagcggattg aaggatgcac gctccgccag tgggcccaag
     8161 gccgggcccg gacaccttcc gaggtgctcc gtcttctgcg ccaggccctc gcggcgctcg
     8221 gcgtcgcgca tcaggcgggg ctggtgcacc gcgacctaaa gcccgagaac atcatggttc
     8281 ggaccgatgg tctcctgaag atcctggatt tcggattggc ccggagcgtc acgcccggcg
     8341 tcgcggcgcg caccgccacc ttgccgggga cgattctcgg aacggcgccg tacatgtcgc
     8401 ccgagcaagt cctcggccag cccgccggtc ccgcgagcga cctgttctcg ctcggctcga
     8461 tcctctacga gctgctgacg ggccagcatc cgttcgcggc gaacagcgcg gtcgagacga
     8521 tgcatcgcat cctgcacgaa tcgccggagg cgccttcgcg cgtgaatcgc gcgctcaccg
     8581 ccgatttcga cttcgtgctc gccaaggcgc tgtccaagga cccgacgcgg cggcatgcgt
     8641 ccgcgcacga tctggatatc gatctcgaga cgctggaatg cggctgcggc acggccgcaa
     8701 cggtggacga cgccggcgcc ggtccgcgca ccctggccgt gcttccgttc aagaacatcg
     8761 ggggcgatcc ggagctgagc tatctgggcg tcgggctcgc ggacgcggtg atcacgcggc
     8821 tctcctcctc gcccgacctg atcgtgcgcg cgaccagcag catcgcgccc taccacaatc
     8881 agccggtcga tccgcgccgc gtgggcgtcg agctggatgc gagcacggtg ctcgacgcct
     8941 ccttccagcg cttcggcgag cgattccgcg cgaccgcccg gctggtggag acggcaagcg
     9001 gccgctcgct ctgggcgggg aaggtcgacc tcaagttcga cgacatcttc gaggtgcagg
     9061 accaggtggc ccacgggatc gccgaagcca tgacggcgcg gctgcagcgg ggagccgcca
     9121 cggtagggcc cgcgcacgcg ccgtcgcccc aggcctacgc gctcctcttg aaggcgcagg
     9181 aggcgtggcg ggattcgagc gaggacggat ttcgtaaggg catcgcgctg gcggaggagg
     9241 ccgtgcggat cgatccggcc ttcgcggacg cctgggcctt cctgggcagc tactaccacg
     9301 gcctcacgga tagcgggtac gatcccgatc cgacttggtt cgccaaagcg gatcacgcgc
     9361 tgggtcaagc cctgtccctc gatcccgaaa accagcaggc gcttttcgcc aaggcagctc
     9421 ttcggctcac ccgcggcgcc aagcgggacg cctacgccag cctcatcgcg gtcgggcgaa
     9481 aggcgcc
//
LOCUS       DASSLZ010000058         4702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_654149, whole genome shotgun sequence.
ACCESSION   DASSLZ010000058 DASSLZ010000000
VERSION     DASSLZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654149"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /gene="thrC"
                     /locus_tag="VFM00_02010"
     CDS             <1..825
                     /gene="thrC"
                     /locus_tag="VFM00_02010"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018521133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET7497050.1"
                     /translation="NARAVLCASTGNTSASAAAYAARRGLRCVVVIPSGQIALGKLAQ
                     AMMYGARVVAIAGNFDRALELVRAMTDDGVAVVNSINPDRIEGQKTIAWEIADALGGR
                     VPDAHALPVGNAGNITATWKGWTERAAALGGPRPRMLGFQAEGAAPIVRGERVLEPKT
                     LATAIKIGNPASWEGALRARDESGGWIEMVSDDEIVAAYKTLAETEGLFVEPASAAGI
                     AGLLKLGAAARLDGVRLAVATVTGHGLKDPERAVAVSPAVTTIGDDEGALRRLILE"
     gene            822..1736
                     /gene="thrB"
                     /locus_tag="VFM00_02015"
     CDS             822..1736
                     /gene="thrB"
                     /locus_tag="VFM00_02015"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET7497051.1"
                     /translation="MIHWSATAPASTSNLGPGFDCLGLALRLPLTVTATRIPEGFRIE
                     RKGEGSDLALDPNRDALLLAYRRLFREAKRPVPTVALSIRSAIPVARGLGSSAAAITA
                     GLTLANHWLRGRFTREELLLLAVELEGHPDNVSASLLGGLTLSMPRADGTTTSFSLPA
                     PRGLAMTLVVPEVRVSTEKARALLPKTVPLAAAAANTARALGLLYAFDRKRPDLLADA
                     LLDVYHVPHRARLIPAYARVAEAGRRAGAYGVTISGSGPTLLALHRPGAGGRIGAAMV
                     RAFARARVRARAIPGRIEPRGASARTLR"
     gene            1754..2971
                     /locus_tag="VFM00_02020"
     CDS             1754..2971
                     /locus_tag="VFM00_02020"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET7497052.1"
                     /translation="MSLLVQKYGGTSVGTPERIAAVADRIVASRRQGADLVVVVSAMG
                     DSTDDLIQLAHKVTTRAHPREMDMLLTAGERISMALVAMAVIDRGAEAVSFTGSQSGI
                     VTDTSHTRAKILEVRADRIREELKKGRVVIVAGFQGVSRDREVTTLGRGGSDTTAVAL
                     AAALQATSCEIYTDVDGVYTADPRIVPGARKLAALSYDEMLELASLGARVLHNRSVEI
                     ARRFGVPIHVRSSFNANEGTRIAKGASMEQVVIRGIAHDADVAKIALLGVPDRPGVAS
                     EIFREIGGHGVNVRMIVQATGAEGRNDVTFAVGAHDAQTVLPLIEQMRKQLGARAFLY
                     DPDVAILSVVGEGLATSPGTAGQVFAALAQAGVNIDLISTSSITITCMVREKDVERAV
                     RSLHEALALEQET"
     gene            2991..3740
                     /locus_tag="VFM00_02025"
     CDS             2991..3740
                     /locus_tag="VFM00_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET7497053.1"
                     /translation="MRLLESPSRLRRGAAPPTERASAVFVGVSSLLHAALLLLFVTWA
                     KPPIDVAIPGGNSRPSGPRSQLVKLSPLRTYPAAEATPPEPEKSRPRPALVKRYTPGA
                     PIVPRPERGPQKKSANDEAKSGAREVATTAASDRAQRWWSPDSSASVGVRVDGDFRWA
                     YYLASIRNKIGQHWVPPAGLGGRAVRATVYFRIGKDGSVSLARLEDGSGVTFFDQTAM
                     RALQSATPLPPLPAGYGGEWLGVHFGFEFQQ"
     gene            3757..>4702
                     /locus_tag="VFM00_02030"
     CDS             3757..>4702
                     /locus_tag="VFM00_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497054.1"
                     /translation="MRGATSLRVLTAVLVLASVLVPMRARTQTDVRTGIKASGTVKAP
                     LLLASFRGGDAAKDAHDVARHDFDLSGVFAVADVEALSKKLEPDPGAQGAVRVEGTVA
                     ESGDGLQFTGEVFDLATAESMFKRSYPFHRADTRETMHRFADDVLEALTGERGICETK
                     VAFIRSTGRVKEVWVMDYDGLNPYQLTHDRSIALSPAWAPWGTELAFTTFKRGNPDLY
                     LFDLRRGASYPFSTRPGPNIAPCYSPDGKWIACSLYRDGNAEIYLISRDAQTAKRLTR
                     NPRIDSGPCFSPSGDQIAFSSDRSGSPQIYVMDGEGVNQ"
BASE COUNT          673 a         1698 c         1665 g          666 t
ORIGIN      
        1 aacgcccgcg cggtgctctg cgcttcgacc ggaaacacct ccgcctcggc ggcggcctac
       61 gcggcgcggc gcgggctgcg ctgcgtcgtc gtgatcccgt ccgggcagat cgcgctcggg
      121 aagctggcgc aggccatgat gtacggcgcg cgggtcgtgg cgatcgcggg gaacttcgac
      181 cgcgcgctcg agctggtgcg cgcgatgacg gacgacggcg tcgccgtggt gaactcgatc
      241 aatcccgacc gaatcgaggg gcagaagacg atcgcctggg agatcgccga cgccctgggc
      301 ggccgcgtcc ccgacgcgca cgcgctcccc gtcggcaacg cgggcaacat caccgccacc
      361 tggaagggct ggaccgagcg cgccgcggcg ctcggcggac cccgtccgcg catgctcggc
      421 ttccaggcgg agggcgcggc gccgatcgtg cgcggcgagc gcgtgctcga gccgaagacc
      481 ctggccacgg cgatcaagat cggcaacccg gcctcctggg agggagccct tcgcgcgcgg
      541 gacgaatcgg gaggatggat cgagatggtc tcggacgacg agatcgtggc cgcctacaag
      601 accctggccg agaccgaggg gctcttcgtc gaacccgcgt cggccgcggg gatcgcggga
      661 ctcctgaagc tgggcgccgc agcccgtctg gacggcgtgc ggctcgcggt ggccaccgtg
      721 accggccatg gcctcaagga tcccgagcgc gccgtcgcgg tctctcccgc cgtcacgacc
      781 atcggcgacg acgagggggc gctccggagg ctcatcctcg agtgatccac tggtcggcga
      841 cggcgcccgc ctccacgtcc aacctgggtc ccggcttcga ctgcctggga ctggcgctcc
      901 ggctgccgct gaccgtgacc gccacgcgca tccccgaggg cttccggatc gagcgcaagg
      961 gcgagggaag cgacctggcg ctggatccga accgggacgc cctcctcctc gcctaccgcc
     1021 gcctcttccg cgaggcgaag cggccggtgc cgacggtggc gctctcgatc cgctcggcca
     1081 tcccggtcgc gcgcggactg ggctcgagcg ccgccgcgat cacggcgggg ctcacgctgg
     1141 cgaaccactg gctcaggggc cgattcacgc gcgaggagct gctcctgctg gcggtggagc
     1201 tggagggcca tcccgacaac gtctcggcct cgctcctcgg agggctcacg ctctcgatgc
     1261 cgcgcgccga cgggaccacg acttcgtttt cgctgccggc gccgcgcggc ctggcgatga
     1321 cgctcgtcgt ccccgaggtg cgcgtgagta cggagaaggc gcgcgcgctc ctcccgaaga
     1381 cggtgccgct cgccgccgcg gccgccaata ccgcgcgcgc cctgggcctt ctctacgcct
     1441 tcgatcgaaa gcggccggat ctgttggccg acgcgctcct cgacgtgtac catgtgccgc
     1501 accgcgcgcg tctcatcccc gcctatgctc gcgtggccga ggcgggacgg cgcgcgggcg
     1561 cctacggcgt gaccatcagc ggcagcggtc ccacgctcct cgccctgcac cggccgggag
     1621 cgggaggacg cattggagcc gcgatggtgc gcgccttcgc acgcgcccgc gtccgcgcgc
     1681 gcgcgatccc aggacggatc gagccgcgcg gggccagcgc gcgcacccta cggtaaggag
     1741 accagcgccc cgagtgtcgt tactcgtcca gaagtacggc ggcacctccg tcggcacgcc
     1801 cgagcggatc gcggccgtcg ccgatcggat cgtcgcgtcg cgccgccagg gcgcggatct
     1861 cgtcgtggtc gtatcggcca tgggcgattc caccgacgat ctcatccagc tcgcccacaa
     1921 ggtcaccacg cgcgcccatc cccgcgagat ggacatgctg ctcacggcgg gggagcggat
     1981 ctcgatggcc ctcgtcgcca tggctgtaat cgaccgcggc gccgaggcgg tctcatttac
     2041 cggtagccag agcggcatcg tgaccgatac atcccatacg cgcgccaaga tcctggaagt
     2101 ccgggcggac cggatccggg aggagctgaa aaaggggcgc gtcgtgatcg tggcggggtt
     2161 ccagggggtg agccgggacc gcgaagtcac gaccctcggg cgcggggggt cggataccac
     2221 cgcggtcgcg ctcgcggcgg ccctgcaggc gacgtcgtgc gagatctaca ccgacgtcga
     2281 cggggtctac accgccgacc cgcggatcgt acccggggcg cgaaagctcg cggcgctctc
     2341 ctacgacgag atgctggagc tggcctcgct gggcgcccgg gtgctgcaca accgttcggt
     2401 cgagatcgcg cgccgcttcg gcgtgccgat ccacgtccgc tccagcttca acgccaacga
     2461 aggcacgcgc atcgcgaagg gagcctccat ggagcaagtc gtcatacgcg gcatcgccca
     2521 cgacgccgac gtggccaaga tcgcgctgct cggcgtgccc gaccggcccg gggtcgcctc
     2581 ggagatcttc cgggagatcg gcgggcacgg cgtgaacgtg cggatgatcg tgcaggccac
     2641 cggcgccgag gggcggaacg acgtgacgtt cgccgtcggc gcgcacgacg cccagacggt
     2701 gctgccgctg atcgagcaga tgcggaagca gctgggcgcg cgcgccttcc tctacgatcc
     2761 cgacgtggcg atcctctcgg tcgtgggcga ggggctggcc acgtcgccgg ggaccgcggg
     2821 gcaggtcttc gcggcgctgg cgcaggccgg ggtcaacatc gacctgatca gcacctcctc
     2881 gattaccatt acctgcatgg tgcgcgagaa ggacgtggag cgcgcggtgc gctccctcca
     2941 cgaggcgctg gccctggagc aggagaccta gtggacggag gccctcctcg atgcggcttc
     3001 tcgaatcgcc gtcccggctc cgacgcggcg cggcaccgcc gacggagcgc gcgagcgcgg
     3061 tcttcgtggg ggtgtcgtcc ctcctccacg ccgcactcct cctcctgttc gtgacctggg
     3121 ccaagccgcc gatcgacgtg gcgatcccgg gcggcaacag ccggccctca ggtccgcggt
     3181 cgcagctggt gaagctctcg ccgctgcgca cgtatcccgc ggccgaggcg acgccgcccg
     3241 agcccgagaa gtcgcggccc cggccggcgc tggtgaagcg ctacacgccg ggcgcgccga
     3301 tcgtgccgcg cccggagcgc ggcccgcaga agaagagcgc caacgacgag gcaaagtcgg
     3361 gagcgcgcga ggtggccacg acggccgcct cggaccgcgc gcagcggtgg tggtcgcccg
     3421 actcgtcggc cagcgtcggc gtgcgcgtgg acggcgattt ccgctgggcg tactacctgg
     3481 cctccatccg gaacaagatc ggccagcatt gggtacctcc tgctgggctc ggtggacggg
     3541 cggtgcgcgc cacggtctat ttccggatcg gcaaggacgg atcggtttcc ctggcgcggc
     3601 tggaggatgg gtccggcgtg accttcttcg atcagaccgc gatgcgagcg ctccaatcgg
     3661 cgaccccgct ccccccgctg cccgcggggt acggcggaga atggctgggg gttcactttg
     3721 gcttcgaatt ccagcagtag atccggcgcg cgcccggtgc ggggtgccac gtcccttcgc
     3781 gtcctgaccg cggtcctcgt gctcgcgtcg gtgctcgtgc cgatgcgcgc gcgcacgcag
     3841 accgacgtgc ggaccggcat caaggcctcg ggcacggtca aggcacccct gctgctcgcc
     3901 tccttccgcg gcggcgacgc ggcgaaggac gcccacgacg tcgcgcggca cgatttcgac
     3961 ctctccggcg tcttcgcggt cgccgacgtg gaggcgcttt cgaagaagct cgagcccgac
     4021 cctggggccc agggcgcggt gcgcgtcgag gggacggtcg cggagagtgg cgacgggctc
     4081 cagttcaccg gcgaagtgtt cgacctggcc accgccgaat cgatgttcaa gcggagctat
     4141 ccgttccatc gcgccgatac gcgcgagacg atgcaccgct tcgcggacga cgtgctcgag
     4201 gcgctgaccg gcgaacgggg catctgcgag accaaggtgg ccttcatccg ctcgaccggg
     4261 cgcgtgaagg aggtctgggt catggactac gacgggttga acccctacca gctcacgcac
     4321 gaccgcagca tcgcgctgtc accggcctgg gccccctggg gcacggagct cgcgttcacg
     4381 accttcaagc gcggcaaccc cgacctctac ctcttcgatc tccggcgcgg agcctcctat
     4441 cccttctcga cccgccccgg gcccaacatc gctccctgct actcgcccga cggcaagtgg
     4501 atcgcctgct cgctctatcg tgacggcaac gccgagatct acctgatcag ccgcgacgcg
     4561 cagacggcga agcggctcac acgaaatccg cgcatcgact ccgggccctg cttcagcccc
     4621 agcggcgacc agatcgcttt ctcctcggac cggtcgggct ccccccagat ctacgtgatg
     4681 gacggcgagg gggtgaacca gc
//
LOCUS       DASSLZ010000059         2859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1288756, whole genome shotgun sequence.
ACCESSION   DASSLZ010000059 DASSLZ010000000
VERSION     DASSLZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288756"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VFM00_02035"
     CDS             <1..1227
                     /locus_tag="VFM00_02035"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET7497055.1"
                     /translation="LVLHQLRAVLGDGAWRRSLKRYVRRHAFGPVETADLRRACEDET
                     GRNLGWFFDQWVHAGGHPEFKVERQWDDASRCLTLTFQQVQPEDRITPVFRVPMQVEI
                     AMSGETRRIPVEIRDRRQTVQIPLPARPRYVALDPEHHVMKLLDFPRSDEELLYGLKR
                     SAFPLERVRCARELAARSDERVVAGLFAALKRDPFWGVRAAAAVSLGEIGARIPGIAE
                     RLGPLALKEQTRVRRGAIWALGWIGDAAAVKLLQRCVARDGSTFNVGWALMGIARARR
                     PDAFERIVAELSRPSHRDMLQILAFDALALLKDPRALDVYLEHTALRYRNEGRAGATK
                     AIGKLGIRTDAAEARLVELLGDRWFRVRNAAAWSLWKIKSPRAEAAIAAALEREALDM
                     CRGAMRDALDGVRKGR"
     gene            1276..>2859
                     /locus_tag="VFM00_02040"
     CDS             1276..>2859
                     /locus_tag="VFM00_02040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7497056.1"
                     /translation="MPLQTGTRLGPYEIVAPLGAGGMGEVYRARDPRLGREVAVKVLP
                     PAFARDRERLQRFEHEARAAGALNHPGITAIFDLGTQDGIPFLVSELLEGESLRTVLA
                     NGPLPPARVADLGIQAAQALAAAHAKGIVHRDLKPENLHVLPDGRLKILDFGLAKLTA
                     TDAPPRDETGPQLHSITMTGTILGTASYMAPEQVRDQPVDHRADLFALGAILYELASG
                     EKAFSGETPADRMTAILTRDPKPLPATLDAAIPGLEAVIRRCLEKRVEERFGTARDLA
                     FTLRLLTDVQERAVPSAPKGSAAAPAPPRDVKFRQLTFREGLVSGARFASDGRTVVYA
                     ATYAGEEPDLYLARVGSHEHRPLHMPGARILSISRADELLVRLRSRDVGGFVNLGVIA
                     RVPLMGGTPREVADEVFQAVFSPDGRQIVAIRFVSGAARLEYPLGTVLAETEGWFSSP
                     AALSDGEVFYVDHPTRGDNAGYPMVAGPRGLRRLSDERFSSVAGGVAFPDRRHVLMSA
                     QQADGSGGLFFVSLEGGYRP"
BASE COUNT          387 a         1034 c         1014 g          424 t
ORIGIN      
        1 ctggtgctgc accagctccg cgccgtgctg ggggacggcg cctggcgccg ctcgctcaag
       61 cgctacgtcc gccgccacgc gttcggcccc gtggagaccg cggacctgcg ccgcgcgtgc
      121 gaggacgaga cggggcggaa cctgggctgg ttcttcgacc agtgggtgca cgcgggaggt
      181 catccggaat tcaaggtgga gcgccagtgg gacgacgcgt cccgctgcct cacgctcacg
      241 ttccagcagg ttcagcccga ggaccggatc acgccggtct tccgggtccc gatgcaggtg
      301 gagatcgcga tgtccggcga gacgcgccgg attccggtcg agatccgcga tcgccggcag
      361 acggtgcaga tcccgcttcc cgcgcgcccg cgctacgtgg cgctggatcc cgagcatcac
      421 gtcatgaagc tcctcgactt cccgcgctcg gacgaggagc ttctctacgg gctcaagcgc
      481 tccgcgttcc cgctggagcg ggtgcggtgc gcgcgcgagc tggccgcccg ctccgacgag
      541 cgcgtcgtgg ccgggctctt tgcggcgctg aagcgcgacc ccttctgggg cgtgcgcgcg
      601 gcggccgccg tgtccctggg tgagatcggc gcccggatcc cgggcatcgc cgaacgcctc
      661 ggtcccctcg ccttgaaaga acagacgcgc gtccggcgcg gcgcgatctg ggcgctgggc
      721 tggatcggcg acgccgccgc ggtgaagctg ctccagcgct gcgtcgcccg ggacgggagc
      781 acgttcaacg tgggatgggc gctgatgggc atcgcccgcg ccaggcgccc cgacgccttc
      841 gaacggatcg tggccgagct gtcccggccc agccaccgcg acatgctgca gatcctggcc
      901 ttcgacgcgc tcgccctcct caaggacccc cgggcgctgg acgtctacct ggagcacacc
      961 gcgcttcgct accggaacga ggggcgcgcc ggcgccacca aggcgatcgg caagctcggg
     1021 atccgcaccg acgccgccga ggcgcgctta gtcgagctgc tcggcgaccg ctggttccgc
     1081 gtccggaacg ccgccgcgtg gtccctctgg aagatcaagt ccccgcgcgc ggaggccgcg
     1141 atcgccgccg cgctggagcg cgaagcgctc gacatgtgcc gcggggccat gcgcgacgcc
     1201 ctcgacggag tgcgcaaggg gcggtaggct cttcgggttc cgtcgcgcat cccttccttc
     1261 cgagaacccg aacgcatgcc gctacagacc ggaaccaggc tgggtcccta cgagatcgtc
     1321 gcgcccttgg gcgccggcgg catgggggag gtctatcgcg cgcgcgatcc ccgtctcggg
     1381 cgcgaagtgg cggtcaaggt gctccccccc gcgttcgcgc gggaccggga gcggctgcag
     1441 cgtttcgagc acgaggcccg ggccgccggc gcgctgaatc acccaggcat taccgccatc
     1501 ttcgacctcg ggacccagga tggaatcccg ttcctggtca gcgagctgct cgagggggag
     1561 agcctccgga ccgtcctcgc caacggcccg cttccgccgg cgcgcgtcgc cgatctggga
     1621 atccaggccg cgcaggcgct cgccgccgcg cacgcgaagg gaatcgtcca ccgggatctg
     1681 aagcccgaga acctccacgt ccttccggat ggccgtctca agatcctcga cttcggcctg
     1741 gccaagctga ccgcgaccga cgcgccgccg cgggacgaga ccggcccgca gctccactcg
     1801 atcaccatga ccgggaccat cctcgggacg gcttcctata tggcgcccga gcaggtgcgc
     1861 gatcagccgg tcgaccatcg cgccgatctc ttcgccctcg gggcaatcct ctacgagctg
     1921 gcgagcggag agaaggcgtt ctccggcgag accccggcgg accggatgac ggcgatcctc
     1981 acgcgcgatc cgaagccgct gccggcgacc ctcgatgccg cgattcccgg cctcgaagcg
     2041 gtgatccggc gctgtctcga gaagcgcgtg gaagaacggt tcggcaccgc gcgggatctc
     2101 gcgttcacgc tgcgcctcct gaccgacgtt caggagcgag cggtgccttc ggctcccaag
     2161 gggagcgcgg ccgcgccggc gccgccgcgg gacgtgaagt ttcgccagct gacctttcgg
     2221 gaggggctcg tctcgggagc gcgcttcgct tccgacgggc gcacggtcgt gtatgccgct
     2281 acctatgccg gcgaggagcc ggacctctat ctcgcgcgcg tgggaagcca cgagcatcgt
     2341 ccgctgcaca tgccgggcgc gcgcattctc tcgatctccc gcgccgacga gctcctggtc
     2401 cggcttcgct cccgcgacgt cggcggcttc gtcaacctgg gcgtcatcgc tcgggtgccg
     2461 ctcatggggg gcaccccgcg ggaggtggcg gacgaggtct tccaggccgt gttcagcccc
     2521 gacgggcgcc agatcgtcgc gatccgcttc gtctcgggag cggcccgcct cgaatacccg
     2581 ctcgggaccg tcctggcgga aacggaggga tggttcagca gtccggcggc cctctcggat
     2641 ggagaggtct tctacgtcga tcatcccacg cgcggggaca acgccggcta cccgatggtc
     2701 gccggcccgc gcgggctgcg gcgcctgagc gacgagcgat tcagctcggt ggccgggggc
     2761 gtggcgtttc cggatcgcag gcacgtgctg atgtcggcgc agcaggcgga cggcagcggc
     2821 ggactcttct tcgtatcgct cgagggcggg taccgcccc
//
LOCUS       DASSLZ010000060         3517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_145867, whole genome shotgun sequence.
ACCESSION   DASSLZ010000060 DASSLZ010000000
VERSION     DASSLZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145867"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFM00_02045"
     CDS             <1..595
                     /locus_tag="VFM00_02045"
                     /inference="COORDINATES: protein motif:HMM:NF018652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HET7497057.1"
                     /translation="VKLQLFGIAHELFLGAGYQMIGMDHFALPSDSLAVAARNRAVFR
                     NFMGYTTHPARDYIGFGVSSIGDLGGAFAQNTKKLNRYKDALDQGLPPIERGFERSKD
                     DEIRRDVIQELMCNFHLDIPQIEKCYGIRFAEYFADALRELDEGGPVQHGFVRRNERE
                     IEVTEAGRLFVRNVCMAFDAYQKKNEAEKKPIFSRTV"
     gene            651..1640
                     /gene="hemH"
                     /locus_tag="VFM00_02050"
     CDS             651..1640
                     /gene="hemH"
                     /locus_tag="VFM00_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET7497058.1"
                     /translation="MKTGILLLNLGGPDTLDAVRPFLVRLFSDREIIRLPGGPVGQWF
                     IGRMIAIKRTKEVQENYHKIGGGSPIVCWSTLQGEGMVARLRARGHDVEYALAMRYWN
                     PDTDAALDRLESLGCDRLVALTMYPHYSVATTGSSMAELLRVMKRRKTRMPLTRIESW
                     YDHPAYLDALASRVRACLDTFPPGSRPTLLFSAHGLPKHFIDDGDPYCDHIRVTLDGI
                     RARLPELPWTLGYQSRVGPVEWIGPSTEEVVDRLGREGVKDVLAVPVSFVSDHIETLY
                     EVDMLYGDQAKKAGIVNFRRPEGLNDFPPFLDALASVVEPALTQAGEAVLAAG"
     gene            2053..2649
                     /locus_tag="VFM00_02055"
     CDS             2053..2649
                     /locus_tag="VFM00_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497059.1"
                     /translation="MPGTDTRPKPWPYAAFTRGREDTFDFYAEGPVDADLTRLPHAND
                     AVFTCVPGEWTSDLHCSLRWQAAPEDSGRYLVRFRARNDLLGGLDTTLIYTIAHDGDQ
                     RPVVTCPDTLNFVVGVRRQVTVSASDPDGDPIFDLQPDSRRVGAFRVLSWAFQFSGSR
                     AGGAGWIVADEAGYTYLRVTATNATNGDGGILLRAAAP"
     gene            complement(2695..>3517)
                     /locus_tag="VFM00_02060"
     CDS             complement(2695..>3517)
                     /locus_tag="VFM00_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7497060.1"
                     /translation="QWSMAERRVSFRTDAPDSLSPPAAYDPPEPADSTRADVRLAPLA
                     PGLWSADLDDLDTRTLVVEFADKLAVLEAAVGSENGERIADAARRQFPNKPIRYFLFS
                     HYHPHYAGGLRAFVAEGATVVTTPGNEHFVRQMADLDFTLRPDRLARAKRPLSMQTFA
                     KRFELADSTNRLVAVNIGPRSDHTNEFVVFWLPRQRMLFETEQGWVTVDGKTRASRRA
                     ERFLKALDDEGLVTDRIVQSWPMRGNPAMLTRVELDSLVTLRGRTLPQTTAGARP"
BASE COUNT          565 a         1167 c         1178 g          607 t
ORIGIN      
        1 cgtgaagctg cagctcttcg ggatcgcgca cgagctgttc ctgggcgcgg gctaccagat
       61 gatcggcatg gaccacttcg cgctgcccag cgattcgctc gcggtcgcgg cgcggaaccg
      121 cgccgtgttc cggaacttca tggggtacac gacgcacccc gcgcgcgact acatcggctt
      181 cggcgtctcc tcgatcggag acctgggagg agcgttcgcg cagaacacga agaagctgaa
      241 ccgctacaag gatgccctgg accaggggct tccgcccatc gagcgcggct tcgagcgcag
      301 caaggacgac gagatccgcc gcgacgtgat ccaggagctg atgtgcaact tccacctgga
      361 catcccgcag atcgagaaat gctacgggat ccgcttcgcc gagtactttg cggacgccct
      421 gcgcgagctg gacgaaggcg gcccggtgca gcacggcttc gtgcggcgga acgagcgcga
      481 gatcgaggtg accgaggcgg ggcggctgtt cgtgcggaac gtctgcatgg cgttcgacgc
      541 ctatcagaag aagaacgaag ccgagaagaa gcccattttc tctcgtacgg tttaaggcgg
      601 tccgggcgaa gcccgggccg tccgggccac tgactctctt atggggatcc atgaagacag
      661 gaatcctgtt actcaacctg ggcggccccg acacgctcga cgcggtgcgc cctttcctgg
      721 tccggctgtt cagcgaccgc gagatcatcc ggcttccggg gggaccggtg ggccagtggt
      781 tcatcggccg gatgatcgcg atcaagcgca cgaaggaagt gcaggagaac taccacaaga
      841 tcggcggggg ctctccgatc gtctgctgga gcacccttca gggcgaagga atggtcgcgc
      901 gcctgcgggc gcgcggacat gacgtggagt acgcgctcgc gatgcgctac tggaatcccg
      961 ataccgacgc ggcgctggac cgcctggaat cgctgggctg cgaccggctg gtcgcgctca
     1021 cgatgtatcc gcactattcc gtggcaacga cgggctcgtc gatggcggag ctgctgcggg
     1081 tgatgaagcg gcggaagacc cggatgccgc tcacgcggat cgagagctgg tacgaccatc
     1141 ccgcctatct cgacgcgctg gcctcgcgcg tgcgcgcctg cctcgacacg tttcctccag
     1201 gatcccggcc cacgctgctg ttctccgcgc acgggctgcc gaaacatttc atcgacgacg
     1261 gcgatcccta ttgcgatcac atccgcgtca cactggacgg gattcgcgct cggctgccgg
     1321 agttgccctg gacgctcggc taccagagcc gcgtcggccc cgtggagtgg atcgggcctt
     1381 cgaccgagga ggtcgtcgac cggctgggac gcgaaggggt gaaggacgtg ctcgcggtgc
     1441 ccgtctcgtt cgtctccgac cacatcgaga cgctctacga ggtcgacatg ctctacggcg
     1501 atcaggcgaa gaaggcaggg atcgtgaact tccgccgccc cgaagggctg aacgacttcc
     1561 cgccgttctt agacgcgctc gcgtccgtgg tcgagccggc cctcacgcaa gccggcgaag
     1621 cagtgctggc ggctgggtag attttcattt cctgacgctt cgagcacgca gcgcgccgtt
     1681 ctcaagatcg tggctcgcct tgtgtccgcg aacgtcattc atgcacgacg tccgtaaagg
     1741 cacttcaccg ccgctggcgg cggtaaaaag tttgccgcaa ttctgcagtt ccattcgcga
     1801 cgcagggaca cgctccagcc actagcatct cgcggcgcag cacccacgct ccagtcgcta
     1861 gcctcccccg ttcggttttc cctcatcgat gcattgaatt caatagggac atcgaatggg
     1921 gagggcacga tgaactccgc tgccgtcggc cgcatcaggc tggccgcctt ggtcttggga
     1981 acgatcctca cgctcagctc ttgcggcgat cctggggacc tcgggtctct gctctcgggg
     2041 ccgatgccag gggtgccggg gacggacacg cggccgaagc cctggccgta tgccgccttc
     2101 acgcgaggac gggaggacac cttcgatttc tatgcggaag gccccgtcga tgccgacctc
     2161 acgcggctgc cgcacgccaa cgatgccgtc ttcacctgcg ttcccggcga atggacgagc
     2221 gatctccact gctcgttgcg ctggcaggcg gcgccggagg attcgggacg ctacctggtg
     2281 cggttccgcg ctcgaaacga tcttctgggc ggtctcgata cgaccctgat ctacacgatc
     2341 gcgcacgacg gggatcaaag gcctgtggtg acctgccccg acacgctgaa cttcgtcgtg
     2401 ggggtccgac gtcaggtgac ggtcagcgcg tccgatccgg atggagatcc gatcttcgat
     2461 ctccagcccg actcgcgccg ggtcggtgcg ttccgtgtcc tgtcctgggc attccagttc
     2521 tccggatcgc gggcgggcgg cgcgggctgg atcgtggccg atgaagcggg ctatacctac
     2581 ttgcgcgtca ccgccaccaa cgcgacgaat ggggatggtg ggatcctgct gcgggcggcc
     2641 gcgccgtgac gatccctacg ggcgcgcccc cgcgcggctg cgccgtgagg gcccctaggg
     2701 gcgcgccccc gccgtggtct gaggcagcgt tctcccccgc agcgtcacca gcgaatccag
     2761 ctcgactctc gtgagcatcg cggggtttcc ccgcatcggc caggattgca cgatgcggtc
     2821 ggtgaccagc ccctcgtcgt ccagcgcctt caggaagcgc tccgcgcgtc ggcttgctcg
     2881 cgtcttcccg tccaccgtga cccatccctg ctccgtctcg aacagcatcc gctgtctggg
     2941 aagccagaag accacgaact cgttcgtatg gtccgatcgc gggccgatgt tcaccgcgac
     3001 cagacggttg gtggagtccg ccagctcgaa ccgcttggca aaggtctgca tcgagagcgg
     3061 ccgcttcgcg cgcgcgagcc ggtcggggcg gagcgtgaag tcgagatcgg ccatctgccg
     3121 cacgaagtgc tcgttcccgg gcgtggtcac gaccgtggcg ccctccgcca cgaacgcgcg
     3181 aaggcctccc gcgtagtgcg ggtggtagtg gctgaagagg aagtagcgga tcggcttgtt
     3241 cggaaactgc cgcctggccg cgtccgcgat ccgctcgccg ttctcgctcc cgaccgccgc
     3301 ttcgaggacc gcgagcttgt ccgcgaactc cacgacgagc gtcctcgtgt cgaggtcgtc
     3361 caggtcggcc gaccagagtc ccggcgccag gggcgcgagc ctcacgtcgg cccgcgtcga
     3421 gtcggcgggc tcgggcggat cgtaggccgc gggcggcgac agcgagtcgg gcgcgtcggt
     3481 gcgaaacgag acgcggcgtt cggccatcga ccactgc
//
LOCUS       DASSLZ010000061         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1544395, whole genome shotgun sequence.
ACCESSION   DASSLZ010000061 DASSLZ010000000
VERSION     DASSLZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544395"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VFM00_02065"
     CDS             <1..686
                     /locus_tag="VFM00_02065"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET7497061.1"
                     /translation="FARVPWKIGEYFQFSIDWNGLNGGSSLMQVQNLHTIDGHRVYRI
                     VTKAESNSFVSRFYKVRDRAESFIDAESLISRRFVKHLREGGFKQDIDVRFEQEQKKA
                     VYAGGPTLDVPAQVHDVLSAFYYVRTRPLAIGSSFTIPTHDNRKSYEMEVKVHKRERI
                     EVPAGKFDCILVEPVLKSEGVFKSKGSIFVWLTDDDRRIPVLVKSKVPVGSISVSLTD
                     MRLSFERTK"
     gene            683..1666
                     /locus_tag="VFM00_02070"
     CDS             683..1666
                     /locus_tag="VFM00_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497062.1"
                     /translation="MRNPLDPKAGRSRYERIDLRGVRTVPVDKRPSKVRVEQFAPAPD
                     PAAPIASLDQFLPDLLTGSALRELLERWVLARTAGRPAVAMIGGHVVKTGVQRPLLSL
                     MEAGAFSALAMNGAAAIHDFEIAFFGATSEPVEETLLTGQFGLVEETPILMNQAVKEG
                     VKRGLGLGESLGEFILDAEAPHASLSLLGQAVARDLPVTVHVAIGTDTIHQHPSFDPA
                     ATGTATHADFTIFAAALREIEDGVVFNFGSAVIMPEVFLKALSTVRNLGHPAARFVAA
                     SFDQIRHYRPEKNVLERPLRAGGKGFAFVGPHELWIPLLAALYLGRAPHAR"
     gene            1663..2676
                     /locus_tag="VFM00_02075"
     CDS             1663..2676
                     /locus_tag="VFM00_02075"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET7497063.1"
                     /translation="MTRPRAWLTLLGLAMSAVAVALLLRSIDAADTWRRLRSADPLWF
                     LGACVVTTASYVVRAVRWGELLSTARRPSFRRLFSATMIGFLAINTLPARLGELVRAY
                     ALSRSERISTATVLGSVAVERLLDLAALGVFWSLSLGVASLPAWFRWSGWITLGLTAG
                     AAAGFWALHRFGGWQALGAETGILRRLPEKPRRALGAAIPAFGEGVRSIASPPILIRA
                     GLLTAAVWIVTAAVFLMTGAALGMNLPVWAPCLMAFIVCVGIMVPSSPGFVGVMEGAC
                     VVGLGFMGVGGPEALAYGVLYHATQLIPLVLLGTWYAVREHVGGEVLHGVPEAPASPK
                     PKK"
BASE COUNT          412 a         1005 c          978 g          521 t
ORIGIN      
        1 gcttcgcgcg ggtgccctgg aagatcggcg agtacttcca gttctcgatc gactggaacg
       61 gcctgaacgg cggctccagc ctgatgcagg tgcagaacct ccacaccatc gatggccacc
      121 gcgtctaccg catcgtcacc aaggccgaat ccaactcctt cgtctcccgc ttctacaagg
      181 tgcgcgaccg cgccgagtcg ttcatcgacg ccgagtccct gatctcgcgc cgcttcgtga
      241 agcacctgcg cgagggggga ttcaagcagg acatcgacgt tcgcttcgag caggagcaga
      301 agaaggcggt ctacgccgga gggcccaccc tggacgtgcc ggcgcaggtg cacgacgtgc
      361 tgtccgcgtt ctactacgtt cgcacacgcc ccctggcgat cggcagctcc ttcaccatcc
      421 cgacccacga caaccggaag tcgtacgaga tggaggtgaa ggtccacaag cgggagcgga
      481 ttgaggttcc ggcggggaag ttcgattgca ttctcgtcga gcccgtgctc aagtccgaag
      541 gcgtgttcaa gtccaaggga agcatcttcg tctggctcac cgacgacgat cggcggattc
      601 cggtgctcgt gaagagcaag gtgccggtcg gctcgatctc ggtcagcctg accgacatgc
      661 ggctctcgtt cgagcgcacg aagtgaggaa tccgctggat ccgaaagcgg ggcgctcgcg
      721 ctacgagcgg atcgatcttc gcggcgtgcg cacggtgccc gtggacaagc gcccgagcaa
      781 ggtgcgcgtg gagcagttcg cgccggctcc cgatcccgcc gcgccgatcg cctccctgga
      841 ccagttcctt cccgatctcc tgaccgggag cgcgctgcgc gagctcctgg agcgctgggt
      901 gctggcgcgg accgccggac ggcccgcggt cgccatgatc ggcggacacg tggtgaagac
      961 cggcgtccag cgccccctcc tctccctgat ggaagcgggc gcgttcagcg cgctggcgat
     1021 gaacggagcg gccgccatcc acgacttcga gatcgccttc ttcggcgcga cctcggagcc
     1081 ggtggaggag acgctcctca ccggtcagtt cgggctggtc gaggagacgc cgatcctcat
     1141 gaaccaggcg gtcaaggagg gggtgaagcg cgggctgggg ctgggcgagt cgctcgggga
     1201 gttcatcctc gacgccgaag cgccgcacgc ttcgctcagc ctgctcggcc aggcggtcgc
     1261 gcgcgacctt cccgtgaccg tgcacgtcgc catcggcacc gacacgatcc accagcaccc
     1321 ctcgttcgac ccggccgcca ccggtacggc cacccacgcc gacttcacga tcttcgcggc
     1381 ggcgctccgg gagatcgagg acggcgtggt gttcaacttc ggctcggcgg tgatcatgcc
     1441 cgaagtgttc ctgaaggcgc tctccaccgt ccggaacctg ggccatccgg ccgcccgctt
     1501 cgtcgccgcg tcgttcgacc agatccgtca ttaccgcccc gagaagaacg tgctggagcg
     1561 gccgcttcgc gccggcggca aggggttcgc gttcgtgggc ccccacgagc tctggatccc
     1621 gctcctcgcc gcgctctact taggacgcgc gccccacgct cgatgacgcg cccccgggcg
     1681 tggctgacgc tcctggggct ggcgatgagc gccgtggcgg tggcgctcct cctccgctcg
     1741 atcgacgcgg cggacacctg gcgccgcctc cggagcgccg atccgctctg gtttctgggc
     1801 gcctgcgtgg tgaccacggc cagctacgtt gtgcgggccg tgcgctgggg ggagctcctg
     1861 tccaccgcga ggcgcccttc cttccggcgc ctcttctccg cgacgatgat cggtttcctg
     1921 gcgatcaaca ccctgccggc ccgcctgggg gagctggttc gcgcctacgc cctctcccgc
     1981 tcggagcgga tctcgaccgc gaccgtcctc ggctctgtcg ccgtcgagcg cctgctcgac
     2041 ctcgcggcgc tgggtgtttt ctggtcgctg tcgcttgggg ttgcgtccct gcccgcctgg
     2101 ttccggtgga gcggatggat caccctgggg ctcacggcgg gcgccgcggc cgggttctgg
     2161 gccctccacc gcttcggggg ctggcaggcc ctgggcgcgg agaccggcat cctacgccgg
     2221 cttcccgaga agccccggcg cgcgctcggg gcggccattc cggcgttcgg ggagggggtg
     2281 cgcagcatcg cgagccctcc catcctcatc cgggccgggc tcttgacggc cgcggtgtgg
     2341 atcgtgaccg ccgccgtctt cctcatgacc ggagcggccc tgggcatgaa cctgcccgtc
     2401 tgggcgccct gcctcatggc gttcatcgtg tgcgtcggga tcatggtccc gtcctcgccc
     2461 ggcttcgtcg gggtgatgga aggcgcctgc gtcgtgggtc tcggcttcat gggggtgggg
     2521 gggcctgagg cgctcgccta cggcgtcctg taccacgcca cccagctcat cccgctggtc
     2581 ctgcttggga cctggtacgc cgtgcgggag cacgtggggg gagaggtttt gcacggcgtc
     2641 cccgaagcgc cggcctcgcc caaaccgaaa aaataggcga cactgacgcc ctaagtcgct
     2701 accccactgt aactgtaaag cttttttagt tgactccggg tggcaccatt gctatactgg
     2761 gtcttgcaat aaatccgcgc tcagagggca cttagggcag gcaagtaccg caatggaggg
     2821 aggatggagt cgtcccaccc gaggacgaat gccattgtcg gtggacatgg cttccatctc
     2881 gttgcattta ctaccctcgt tgcggctttg agtttc
//
LOCUS       DASSLZ010000062        17794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_656163, whole genome shotgun sequence.
ACCESSION   DASSLZ010000062 DASSLZ010000000
VERSION     DASSLZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 17794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17794
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656163"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFM00_02080"
     CDS             <1..237
                     /locus_tag="VFM00_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HET7497064.1"
                     /translation="LPAGVGANRIAVSLVEAWRGEVCHAAVTDDRGRFRRYAIVDPSF
                     HNWPGLAMAMRDQPVSDFPLCNKSFNLSYSGYDR"
     gene            299..1027
                     /locus_tag="VFM00_02085"
     CDS             299..1027
                     /locus_tag="VFM00_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7497065.1"
                     /translation="MPAAGPVLPASFRGRPVLDPARCVDGCRACAERCPTSAIRVEPR
                     DGGGAARLSLDLGRCLFCNECVEGCPSGAVRFGKDYRMAARAREDLVADEGPYRLAGA
                     LDAEARRLFGRSLRLRHVSAGGSAGCEQEANALGNVVFDMGRFGIQFVASPRHADGLL
                     ITGPVPRNMKLALEKTYEAVPAPKIVVAVGAAAISGGPFAGDPEVQDGAASVVPVDLF
                     VPGHPPHPLTILDGLLRLLGRIRE"
     gene            complement(1093..1293)
                     /locus_tag="VFM00_02090"
     CDS             complement(1093..1293)
                     /locus_tag="VFM00_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7497066.1"
                     /translation="MAAGTVKWFNEAKGFGFIQQEGGEDVFVHYSAIMGEGFKTLAEG
                     ERLEFDIVRGPKGLQAANVRKA"
     gene            1615..2130
                     /locus_tag="VFM00_02095"
     CDS             1615..2130
                     /locus_tag="VFM00_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7497067.1"
                     /translation="MKRIIATMAITLCAVVLTGRASADESDKAPQGKEIFTKYKCQGC
                     HSVDAAQIKRTKPVSATMKHKPPDLSTIGDTRGHEWIEKWLDKMEKVDTRSHPTLKFK
                     GTKDEKEALAAWLETMKTPGGTGAAGAAEQAKDAAKEAKQDAKAADEAAEKAKAAAEE
                     AKEKADTTHAH"
     gene            complement(2212..3519)
                     /locus_tag="VFM00_02100"
     CDS             complement(2212..3519)
                     /locus_tag="VFM00_02100"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET7497068.1"
                     /translation="MATLVHDTKKSTTATLSLDGTMATLPVVRGTEGETAIDIEELRA
                     ATGLITLDPGFANTGSCKSAITFIDGEKGILRYRGYPIEELAAKSSFLEVAWLLIHGE
                     LPTNAELESFTNEITRHTMLHQDFERFFGALPKDAHPMPVCAAAVGALATFYQQPESE
                     AQIHDTIIRLIGKMPTIAAYSYKHSIGQPFVYPLNRLDYASNLLRMMFSVPSEEYEVD
                     PVLAKAVDLLLILHADHEQNCSTSTVRVVGSSKANLYASISAGISALWGPLHGGANQQ
                     VIEMLERIEAEEGSADKFIAKAKDKNDTTRLMGFGHRVYKSYDPRAAILKKSCGEVLQ
                     RVGVSSKLLEIAMRLESVALKDEYFVSRKLYPNVDFYSGVIYKALGIPVNMFTVMFTL
                     GRLPGWVAQWLEMRRDPDFRISRPRQIYVGSPKRMYVPLPDRA"
     gene            complement(3778..5085)
                     /locus_tag="VFM00_02105"
     CDS             complement(3778..5085)
                     /locus_tag="VFM00_02105"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET7497069.1"
                     /translation="MTTQLKDAKSPETATLSLNGTSATLPVIRGTEQETAIDIEKLRA
                     TTGLITLDPGYGNTGSCRSAITFIDGEKGILRYRGYPIEELAQKSTFLEVAWLLIHGE
                     LPARADLDGFTAEITRHTMLHKDFDRFFDALPKDAHPMPVCAAAVGALATFYQKPESE
                     AQIHDTIIRLIGKMPTIAAYSYKHSIGQPFLYPMNRLDYVANFMRMMFGVPSEEYEVD
                     PVLARVLDLLLILHADHEQNCSTSTVRMVGSSMANLYASIAAGISALWGPLHGGANQQ
                     VIEMLEQIALQEGSADKFIAKAKDKNDTTRLMGFGHRVYKSYDPRAAILKQACADVLK
                     RVGRSSQLLDIAMRLEEVALHDDYFVSRKLYPNVDFYSGVIYQALGIPVNMFTVMFTL
                     GRLPGWIAQWLEMRRDKDFRISRPRQVYVGSPKRMYVPLADRG"
     gene            5467..14508
                     /locus_tag="VFM00_02110"
     CDS             5467..14508
                     /locus_tag="VFM00_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HET7497070.1"
                     /translation="MRTPGAALLSLFAALSIAAAPGFAEPPSKPGEVILKFRAGTPQS
                     VKDSILADIQASTIRRFGRIRAEQDRAGIGTDEAIRRYKNHPSVEIIEPNYILSVNRT
                     PNDPLFGQLWGMNNTGQTGGLPGADISAVHAWDVQTGSSSVVVGIIDTGVDMTHPDLA
                     ANIYTNPGEIAGNGVDDDGNGFIDDVHGWDFVNHDNDPSDDYGHGTHVAGTIGAVGNN
                     GIGVAGVAWSVRIMPIKFLDSSGSGSTADAIACVEYATMMGVTLTSNSWGGGGFSEAL
                     RQAIEDAGSAGILFVAAAGNAGMNTDVSVNYPSGYDLPNIIAVAATDDDDHLASFSNY
                     GAVSVDLGAPGVDILSSVPGGTYATKSGTSMATPHVSGILALMKSEYPLMNAAGLKTR
                     LLGTVDPIPELAGKTVSGGRANAFRALERPDSIPPESVADLRLAETWSGGLRVEWTAS
                     GDDGATGRASGYELRYSTFPLTAANFGTGTLASAPDPSPSGALETASIVGLGFSTTYY
                     VALRVLDEMGNVSGLSNVVSGATLGIPALAFAPDSVTASLFTGGTAVRPLTVTNTGEG
                     TLDFTLSEAQAAATVTVKAFTPLARGEADPRVGDPVVQDAGGPDTFGYRWTDSDEPGG
                     PGFNWVEISGVGTRIPMSFLDENFGPIPIGFEFPFYGKPFTNLHACTHGWVSFTNGGV
                     QYTNQPLPNAGAPENLLAIFWEDLNFGGVQRAYYHYDGTRFILEYLNVPRTGGGATYT
                     FEVLLYPNGDIVYQYLSMAGVTDGGTIGIQNATRDDGLQIDFNASYVHDGMAIRISKQ
                     PDWFSVTPPSGRVLAGHSLDLQVAIDAADLTGALYPGTIFLHTNDPARPEAAVPVRLS
                     VTGVADIDLSRAAFDFGPVFVGGTRADSLVVTNRGTDVLHVTGLSAAPSVFTASPGSL
                     TLAPREARKAYVTFAPSSAVPFSGALTIVSDDPDEATVTIPLAGAGLVPPDIAVAPES
                     IAVSLFTGESATRSAEVRNEGGSDLTFEIGYALGHSAAEAPVATAPATETRDLLASYE
                     RFASGQGDPFVAASPSPRPGTLPLGERMEQALADSVPVFFDDMEHGIGGWTHSAVSGT
                     VDLWNPSTARASSGAKSWRVQQHESYGSDALRSQPIDLRFYRNAMLAFQQWYDFDDCG
                     GDPTFEPDGAIVEVSTDGGGTWTQVAPMGGYPYVLDDICGNPLANRPAYSHTGGVGSA
                     FIPAVFDLTPFAGQILQIRFRAGWDCGNCAANEGWYLDDVSVYSTMPRIVSVSPVEGT
                     IPAGGALGLSLAFDASGLFGGDYTNDVLVRSNDPDENPAVIATSLHVTGAPDVAWDPP
                     AVEFGARFIGGAATDTLAVFNAGTDVLHVTEVTLDRPEYQVETAPFTLAPGARHDLHV
                     TFRPVATGFLTASLILRSDDPDEPNAAVSIRGEGVVPPSAGLAPASIAATVETGQTAT
                     RTLTLRNDGGADLVFQAHGDDPASAPPADSSVAIPDSLRGPTANTLAPTPGEPQVQAV
                     YAGTYLRFGVTSFGEIMPYQYPLGNEHLQVGTYVSGYTLAYQSGGVDHIAFAFVSDHY
                     GVAPEQYREVENSPARVVVEVSVRTADGLLRVVRTITFERAARAVRVDTRLENVAGTT
                     LGAVVFKEEADWDVDQNYNDDWDYDRGRHLIYAKSIHVAGLASAEAPDLMDIYGWNDL
                     TSRSTFVDFPAAPVLSFDGLALLHFDLGPMAPSDARTIAVAYGAADDVNQLRAALSDA
                     LRGLTWLSVTPASGTVPAGGSIDLTARFDPGRRSSGDYDGRVVVDSNDPVHPRLLASA
                     VMTVIGRPLFTAVPAALAFDSIFVGATLRDSLRVRNGGVDPLLVSGVTSPDPSYAASP
                     SAFAVAPGDSQLVVVTFHPLTAGDRTSTLQFAHNAAGSPSSVGVSGFALVPPDIAADP
                     ASFTVDTETGRVETRTLTVRNDGGSELVFQASTLGLSEAALPGTPVSLPDSLQGRATP
                     NTTAPSAPSAVQAIYEGTYLRFGITSYGELMPYQYPLGVEHLELGATVSGYTVAYRNG
                     GHDHLAYSVDFERSNVVAESYATLENSPQRVIVLVTTKTQDGIVRIRRRFDFERSRRG
                     VRIETAIENLTATSLTDVVFKEVADWDMDGEFNNVWNYDPANAMVYASRTHYGAIGSK
                     QPATIMDLYGWGDYSSRSTFVDIPSGPVSTDGLALLHFALGDVPPAEATSTVITAYAM
                     GGDLGQLRESMALAVGSASWLAVSPKTGRVPAGGAMDLTVRFDPERRPTGEVRGEIWL
                     ESNDPDEVLVQLPATMRVTARPVIAVTPAALDFGIVFLNESATRTFRVRNDGVVPLHV
                     TSIGASDPFYAATPATLTVAPLDSQQVTVTFTPVTVGLRNASLALTHDAAGSPTPVAL
                     TGTGVVPPTISFLPASVEAAVRSGQGGTTTLSVSNAGAAPLTFTIAAPPPFPSAFQAM
                     SYQELRKGEKDARPGILGTGGADRFGHFWIDSDQPGGPAYEWSDVRGIGTHVPFAPVT
                     DDATLGPFPIGFDFPWFGGVVHEFRVCSNGWISFTNPLAAFGNQPLPNAGPGVPENLI
                     AALWDDLLLDASAGSSVDVWGDTEHLVVQYTNVRPLGVLSPTPHTFQIVLRRSGEIRL
                     FYQSVGSLLSGATVGIQNGTRDDGLTVSFNTPYLHDQLAVRIVKPAPWLIVTPMTGSV
                     LPASSLPLSLQFDPRELTDGDHEADLNVASNDPAHRSVSVPVLLHVGETPATASDFDP
                     NTVNKESNGRYAVMRAELPPGVDPWKVAIETVRFQETLAPAEDSLAIEDWNANGIPDL
                     RFRFHRDQVEGILPEGERVPVVLTGRVGNQTWFIARDSIRVIRPRVLAPNGQEVLVPN
                     TTYTVSWNNPQLGAPDEAALYYSTDRDSSWNLIAEHVTGTSYRWLVPAVSAPWARLRV
                     YLFDKQGVMGYDASDQSFAIVASPTGVTVEETAPPASFALRQNRPNPFNPSTEIGFDL
                     PLAARVRLQIFQPNGRLVRTLVDEPLSAGRYRAPWDGRDDRGRAMASGVYLYRISAGP
                     STESRRMLLLK"
     gene            complement(14577..15419)
                     /locus_tag="VFM00_02115"
     CDS             complement(14577..15419)
                     /locus_tag="VFM00_02115"
                     /inference="COORDINATES: protein motif:HMM:NF025420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497071.1"
                     /translation="MDARLIVGEGFPPIWLGTTVAPDRPYEVASARLRDASVILTTMG
                     GDTIRYSDSGIYPYQPVGYTPGVARVEPLTTYRLHVRAPDGRVVTAETTTPDTFHVRE
                     WLLVDDRTLEVRRRLAVPEFPEEVLAYDSVYSSNRLVYQDGLVEARFDRDGSMAFQMA
                     LFSLDDNSPLLIDADFLSEADKASLERQSASPPLDAPDGFVRLPWLAVWYEGRHRFVV
                     YSVDRNWYDLARSINFYAPAGLGFGSNAGDEFQRPIFHVNGGIGIFGSAAMDWTGFTV
                     FPRP"
     gene            complement(15527..>17794)
                     /locus_tag="VFM00_02120"
     CDS             complement(15527..>17794)
                     /locus_tag="VFM00_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7497072.1"
                     /translation="RPMAAVACLLLDLVLGGTIATAGSLEGFVRAKGEAEGPAYAYIA
                     LTPLAAGDSAAAARAGLRAVSNPTGFYSFGGITPGEYRRTCRAAGYRDFADTVAIGEG
                     TLRRDIVLATSPFPVSAVDVRGEREPRDVRGTPSYVELPARELKRLPAVGEADLIRSL
                     QLLPGVQSASDFSSGLYIRGGGPDQTLILLDQIPLYNPTHAFGFFSTFNPAAIKDVAL
                     YKGAYPSQYGGRLGSVLDVENRDGSRDGLRGAGAVSLIAGRATVEGPIRNGSWILSAR
                     RTYLEPVLNAVRNDSTEIPSYFFYDLNGRVHRSMSPRDDLSFSGYRGRDKLHLDLDRG
                     TFVDIRWGNVAGMGRWMHDFGRGAIGNLIVSATEYKSDTDVKIFSTPLFFGNRLLDVS
                     AKGDLSWNPATGHAARTGVSVSAYRFELGQQFNGVEQPGFDERPVGTAAFVEDQWTPN
                     VLWTVRPGVRAEVFGPRGHVRVEPRLSLNHVLGPNVRVKAGGGGYTQHLQLVSTEAFN
                     GTDFWVPTDGSAKPGRSWQAVSGLEWLPTDAYTVSVEGYYTWLRNLVQLDNTRSADAQ
                     GTSTEDLFYTGGRGWASGVELFAQKRRGALTGWVGYTLGWSRRRWPELNEGQTFPPKY
                     DRRHDVKVVAQWNRSRWSFGADFVFATGQAFTPLESRYGLNDPATERLHDGLVLPGDR
                     NSSRLLPYHRLDISVTRHGHLFGVPVDYYVQVFNAYDRKNEWFVQYDTDNPETEPKIV
                     HQLPLVPTIGINFDR"
BASE COUNT         2806 a         6130 c         6009 g         2849 t
ORIGIN      
        1 cttcctgccg gtgtgggcgc gaaccggatc gccgtgtcgc tggtcgaggc ttggcgcggg
       61 gaagtctgcc acgccgcggt caccgacgac cgcggacgct tccgccgcta cgccatcgtg
      121 gatccctcgt tccacaactg gcccgggctc gcgatggcca tgcgcgatca gccggtctcc
      181 gactttccgc tctgcaacaa gagcttcaac ctctcgtact cgggatacga ccggtgagga
      241 ggggcgcgtg ctgaaggcct tcgtggagcg catgcggcag gggcggcgga cgatcccgat
      301 gcccgccgcc ggcccggtgc tgcccgcgtc cttccggggc aggccggttc tggatccggc
      361 gcgatgcgtg gatggatgcc gcgcctgcgc ggagcgctgc ccgacgtcgg ccatccgcgt
      421 cgagccgcgc gacgggggag gagccgctcg actctcgttg gatcttgggc gctgcctctt
      481 ctgcaacgag tgcgtcgagg gctgccccag cggggcggtg cgcttcggca aggactaccg
      541 gatggccgcg cgcgcgcggg aggatctcgt cgcggacgag ggaccgtatc gcctggcggg
      601 ggcactcgac gccgaggcgc gaaggctctt cggccgctcg ctccgcctgc ggcacgtgag
      661 cgccggggga tcggcgggtt gcgagcagga agcgaacgct ctcggcaacg tcgtcttcga
      721 catgggacgg ttcgggatcc agttcgtggc ctcgccccgc cacgccgacg ggctcctgat
      781 caccggcccg gtgccgcgga acatgaagct cgcgctggag aagacctacg aggccgtgcc
      841 cgcgccgaag atcgtggtgg cggtgggcgc cgcggccatc tccgggggac ccttcgcggg
      901 ggatccggag gttcaggacg gggccgcgtc ggtcgtgccc gtggatctct tcgtcccggg
      961 ccacccgccc catccgctca ccatcctgga cgggctgctc cggctgctgg gacggattcg
     1021 cgagtagaaa cgcgaacggg gcggagcctt tcggccccgc cccgagctgt tccgtggttc
     1081 ggctcggcta ggctatgcct tccggacgtt ggccgcctgc agacccttcg gtccgcggac
     1141 gatgtcgaac tccagccgct cgccttcggc cagcgtcttg aagccctcgc ccatgattgc
     1201 cgagtagtga acgaagacgt cctcgccacc ctcctgctga atgaacccaa agcccttcgc
     1261 ttcgttgaac cacttcacgg tacctgcagc cacactgtgc tccttgctct tcggggacga
     1321 ttgccccaac tcgccgccct ctcggaacgg ccgacccggc cactttggcc gagcctccgg
     1381 acgagacgtc cggatacaga aaaagccgca ggaaggcacc ccctgcggcg tcatttcgcg
     1441 tcgttgcgct gcactctgac cgtcaggcga cttctactgc ccgggagtat cggcagcccc
     1501 gggtgggaag taaagtcttt tgttgagccc cctctcgacg ggcgcccggg cactgttatg
     1561 cttctgggta cgtgggctgc ccacgctggg agtgcttcac aaggaggacg aatcatgaag
     1621 cgcatcatcg ccacgatggc catcacgctc tgcgcggtcg tgctgaccgg ccgggcctct
     1681 gccgacgagt ccgacaaggc gccgcagggc aaggagatct tcaccaagta caagtgccag
     1741 gggtgccact ccgtggatgc cgctcagatc aagcggacca agcccgtctc cgcgaccatg
     1801 aagcacaagc cgcccgacct ctccaccatc ggagatacgc gggggcatga gtggatcgag
     1861 aagtggctgg acaagatgga gaaggtggat acgcgctccc atccgaccct gaagttcaag
     1921 ggcacgaagg atgagaagga ggcgcttgcc gcctggctcg agacgatgaa gacgccgggc
     1981 gggacgggcg ccgccggcgc cgccgagcaa gccaaggacg cggccaagga agcgaagcag
     2041 gatgccaagg ccgccgacga ggccgcggag aaggccaagg ccgcggccga agaggccaag
     2101 gagaaggcgg acaccacgca cgcccattga ttccgagcgt ctcacgaaac gaggccccgg
     2161 ccgctcgcgc gaccggggcc ttcgtccatc aggccggatg gatgccgcgc gtcaggcgcg
     2221 gtcgggaaga ggcacgtaca tccgcttcgg cgagcccacg tagatctgcc gcggccggga
     2281 gatgcggaaa tcaggatccc gccgcatctc gagccactgc gcgacccagc cggggagccg
     2341 tcccagcgtg aacatgaccg tgaacatgtt caccgggatg ccgagcgcct tgtagatcac
     2401 gcccgaatag aagtcgacgt tcgggtagag cttccggctc acgaagtact cgtccttcaa
     2461 ggcgaccgac tcgaggcgca tcgcgatctc gaggagcttg ctcgagacgc cgacgcgctg
     2521 gagcacctcg ccgcaggatt tcttcaagat ggccgcgcgc gggtcgtagc tcttgtagac
     2581 gcggtggccg aagcccatga gccgcgtggt gtcgttcttg tccttcgcct tcgcgatgaa
     2641 cttgtccgcc gagccctcct cggcctcgat ccgctccagc atctcgatga cctgctgatt
     2701 cgcgccgccg tggaggggac cccaaagagc gctgatgccg gcggagatgg aggcgtagag
     2761 gttggccttg gagctgccca ccacgcgaac ggtgctggtg gagcagttct gctcgtggtc
     2821 ggcgtggagg atcaggagca ggtccaccgc cttggcgagc accgggtcca cctcgtactc
     2881 ctcgctgggc accgagaaca tcatgcggag caggttcgag gcgtagtcca gtcggttcag
     2941 cggatagacg aacggctgcc cgatcgagtg cttgtagctg tacgccgcga tggtcggcat
     3001 cttgccgatc agccggatga tcgtatcgtg gatctgcgcc tcgctctccg gctgctggta
     3061 gaacgtggcg agcgcgccca cggcggcggc gcagaccggc atcgggtgcg cgtccttcgg
     3121 cagtgcgccg aagaagcgct cgaagtcctg gtgaagcatc gtgtggcggg tgatctcgtt
     3181 cgtgaagctc tcgagctcgg cgttcgtggg cagctcgccg tggatcagga gccaggccac
     3241 ctcgaggaac gagctcttcg cggccagctc ctcgatcgga tagccgcgat agcggaggat
     3301 ccccttctcg ccgtcgatga aggtgatcgc gctcttgcac gagccggtgt tggcgaagcc
     3361 gggatcgagg gtgatcaggc cggtggcggc ccgaagctcc tcgatgtcga tcgcggtttc
     3421 cccctccgtg ccgcgcacga cgggcagcgt ggccatcgtc ccgtccaggg acaacgtggc
     3481 cgtcgtggat ttcttcgtgt cgtgaaccag ggtcgccatg gggctctcct cctgacgggg
     3541 tcgtcatgga ccctgccagg ttgtcagagg acaacagcgt tcgccggcgg cgcgcccggg
     3601 ggataggcgc atggaaccgc ggcgtttatc gaatcgagtg cgagaacgtc gggggagtga
     3661 gccgcatgaa cagtgccgga cgcgcggcac acgccgccgg cgaaacggcg agggccccgc
     3721 cgaccgctcg gagccctcgc gacgaagtgc cggagctcgg ctccggcaag gctgaggcta
     3781 tccgcgatcg gccagcggga cgtacatccg cttcggcgat cccacgtaca cctggcgcgg
     3841 tcgcgagatg cggaaatcct tgtcgcggcg catctccagc cactgcgcga tccagcccgg
     3901 aagccggccc agcgtgaaca tgaccgtgaa catgttcacc gggatgccga gcgcctgata
     3961 gatgaccccg gagtagaagt cgacgttggg gtagagcttg cggctcacga aatagtcgtc
     4021 gtgcagggcg acctcctcga gccgcatggc gatgtcgagc agctggctcg agcgtcccac
     4081 gcgcttcagc acgtcggcgc acgcctgctt caagatggcc gcgcgcgggt cgtagctctt
     4141 gtagacgcgg tggccgaagc ccatgagccg cgtggtgtcg ttcttgtcct tggccttcgc
     4201 gatgaacttg tccgccgagc cctcctgcag agcgatctgc tccagcatct cgatcacctg
     4261 ctggttcgcg ccgccgtgga gcgggcccca gagcgcgctg atcccggccg cgatgctcgc
     4321 gtacaggttg gccatcgagc ttccgaccat gcgcaccgtg ctggtggagc agttctgctc
     4381 gtgatcggcg tgcaggatca gcaatagatc gagcacgcgc gcgagcacgg gatccacttc
     4441 gtactcctcg gacgggacgc cgaacatcat ccgcatgaag ttggcgacgt agtcgaggcg
     4501 gttcatcggg tacaggaacg gctgtccgat ggagtgcttg taggagtagg cggcgatcgt
     4561 cggcatcttg ccgatcagcc ggatgatggt gtcgtggatc tgcgcttcgc tctcgggctt
     4621 ctgatagaag gtcgcgagcg ctccgaccgc ggccgcgcag accggcatcg ggtgcgcgtc
     4681 cttggggagc gcgtcgaaga agcggtcgaa atccttgtgc agcatggtat gccgcgtgat
     4741 ctcggccgtg aatccgtcca ggtcggcgcg ggccggcagc tcgccgtgga tcagaagcca
     4801 cgccacctcg aggaaggtgc tcttctgcgc cagctcctcg atcgggtacc cgcgatagcg
     4861 caggatccct ttctccccgt cgatgaacgt gatcgcgctc cggcacgagc cggtgttgcc
     4921 gtagcccggg tcgagcgtga tgaggcccgt cgtggcccgg agcttctcga tgtcgatcgc
     4981 ggtctcctgc tcggtgccgc ggatcaccgg gagcgtggcg gaagtgccgt tcaaggacag
     5041 ggtggcggtc tcgggactct tggcgtcttt gagctgggtg gtcatggcgc tccctcccgg
     5101 agggcccgtt ccgaacccag ccaggcattt tcggtatcgg tgttacggcg ggccgtgcgg
     5161 tatggggccg aggccgcgtt atccgtatgt ccagcatcat gggggaatcc cgggaggcgc
     5221 acaaggcggg accggttcac cgttggtcgc tccgccacgg ggactcgtgt ccgtcgtgga
     5281 tgcgaccaga ggaccattga ttcacgccgg ggccggacgt acggtgcctt gaatctcccg
     5341 tcccgttttg tgcgggacca ccacgacatt caaagatctc ctcccccccg gcgaggcgcc
     5401 actgcgggcc gtcccgccag cgagcacgcc cggcccgcaa gcgcccccgt ggcgaggaga
     5461 agttccatgc ggacccccgg tgccgctcta ctctccctgt tcgctgccct ctcgatcgcc
     5521 gcggccccgg gcttcgccga gcctccgtcc aagccgggcg aggtcatcct caagttccgc
     5581 gccgggacgc cgcagagcgt caaggactcc attctcgccg acatccaggc ctccaccatc
     5641 cggcgcttcg gccggatccg ggccgagcag gaccgcgccg gcatcggcac cgacgaggcg
     5701 atccgccgct acaagaacca ccccagcgtg gagatcatcg agccgaacta catcctgtcg
     5761 gtgaaccgga cgccgaacga tccgctcttc ggccagctct gggggatgaa caacaccggc
     5821 cagaccggcg ggctgcccgg agccgatatc agcgcggtcc acgcgtggga cgtccagacg
     5881 ggctcctcgt cggtggtcgt ggggatcatc gacacgggcg tcgacatgac ccatcccgac
     5941 ctggccgcca acatctacac gaatccagga gagatcgcgg gcaacggcgt cgacgacgac
     6001 gggaacggct tcatcgacga cgtgcacggg tgggacttcg tcaaccacga caacgacccc
     6061 tccgacgact acgggcacgg cacgcacgtg gcggggacga tcggcgcggt ggggaacaac
     6121 ggaatcggcg tggccggggt ggcctggtcg gtgcgcatca tgccgatcaa gttcctcgac
     6181 tcgagtggat ccggatccac cgcggacgcg atcgcctgcg tcgaatacgc gacgatgatg
     6241 ggcgtgacgc tgaccagcaa ttcgtggggc ggaggcggct tctccgaggc gctccggcag
     6301 gccatcgagg acgcgggctc ggcggggatc ctcttcgtgg ccgccgccgg caacgccggc
     6361 atgaataccg acgtcagcgt caattacccc tccggctacg acctgcccaa catcatcgcg
     6421 gtcgcggcga ccgacgacga cgaccacctc gcgagcttct ccaactacgg cgccgtgtcc
     6481 gtggacctgg gcgccccggg agtggacatc ctgagctcgg tccccggggg cacctacgcg
     6541 accaagagcg gcacctcgat ggcgacgccg cacgtgagcg gcatcctcgc cctcatgaag
     6601 agcgagtacc cactcatgaa cgcggccggg ctcaagacgc gcctgctcgg caccgtggac
     6661 cccatcccgg agctcgcggg aaagacggtc tccggcggac gcgcgaacgc gttccgggct
     6721 ctcgagcggc cggacagcat tccgcccgag tcggtggcgg atctgcgcct ggccgagacc
     6781 tggtcggggg ggctccgcgt ggagtggacc gcctcgggag acgacggcgc gaccggaagg
     6841 gcgtccggct acgagctgcg ctactccacg ttcccgctga ccgccgccaa tttcgggacg
     6901 ggaacgctgg cgtccgcgcc ggatccctcg ccttcgggcg cgctggagac cgcctcgatc
     6961 gtcgggctcg gtttctccac gacctactac gtggcgctcc gggtcctgga cgagatgggc
     7021 aacgtgtccg gcttgtcgaa cgtggtctcg ggagccacgc tgggaattcc ggcgctggcg
     7081 ttcgctccgg attcggtcac ggcctcgctc ttcacgggcg gcacggcggt gcgcccgctg
     7141 accgtgacca acaccgggga ggggacgctc gacttcaccc tctcggaggc gcaggccgcc
     7201 gcgaccgtca cggtgaaggc gttcactccg ctcgcgcgcg gcgaagccga tccgcgggtc
     7261 ggcgaccccg tggtgcagga cgccggcgga ccggacacgt tcggctaccg gtggaccgac
     7321 agcgatgagc ccggcgggcc gggcttcaac tgggtggaga tcagcggggt cgggacgcgc
     7381 atccccatga gcttcctcga cgagaacttc ggcccgatcc cgatcggatt cgagttcccc
     7441 ttctacggga agccgttcac caacctgcac gcctgcacgc acgggtgggt ctcgttcacc
     7501 aacggcggcg tccagtacac gaaccagccg ctgccgaatg cgggcgctcc ggagaacctg
     7561 ctcgcgatct tctgggagga tctcaacttc ggcggcgtgc agcgggcgta ttaccactat
     7621 gacggaacgc gattcatcct cgagtacctg aacgtgcccc ggaccggcgg cggcgcgacg
     7681 tacacgttcg aggtgctgct gtatcccaac ggcgacatcg tctaccaata cctcagcatg
     7741 gcgggcgtca cggacggcgg gacgatcgga atccagaacg cgacccgcga cgacggcctc
     7801 cagatcgact tcaacgcctc gtacgtgcac gacggcatgg cgatccgcat ctcgaagcag
     7861 cccgactggt tctcggtgac gcccccgtcc ggccgcgtgc tcgccggcca cagcctcgat
     7921 ctgcaagtcg ccatcgacgc ggcggacctg acgggcgccc tctacccggg gacgatcttc
     7981 ctccacacca acgatccggc gcggccggag gcggcggttc cggtccggct ctcggtcacc
     8041 ggtgtcgccg acatcgacct gagccgcgcg gcgttcgatt tcggccctgt cttcgtgggc
     8101 ggaacccgag ccgactcact cgtggtcacg aaccgtggca ccgacgtgct ccacgtgacc
     8161 gggttgtccg ccgcgccctc cgtgttcacc gcatcccccg gctccctcac cctggcgccc
     8221 cgggaggcgc gcaaggccta cgtgacgttc gccccatcct ccgccgtgcc cttctcggga
     8281 gcgctcacga tcgtgagcga cgatcccgac gaggcgacgg ttacgattcc tctcgccggc
     8341 gcggggctcg tgcccccgga catcgcggtc gctcccgaat cgatcgcggt ctcccttttc
     8401 accggagagt ccgcgacgag aagcgcggag gtccgaaacg aaggcggcag cgatctgacg
     8461 ttcgagatcg gctatgcgct gggccactcg gcggcggaag cgcccgtggc cacggcgccg
     8521 gccaccgaga cgcgggatct cctcgcgagc tacgagcgat tcgcctccgg ccagggcgat
     8581 ccgttcgtcg cggcgtcgcc gagcccccgg ccgggaacgc ttcccctcgg cgagcggatg
     8641 gagcaggccc ttgccgacag cgtgccggtc ttcttcgacg acatggagca tgggatcggc
     8701 gggtggaccc attccgccgt gtccgggacg gtcgacctct ggaacccgtc gacggcgcgc
     8761 gcctctagcg gggccaagag ctggcgggtg cagcagcacg agagctacgg ctcggacgcg
     8821 ctccgctctc agccgatcga tctgcgcttc taccgcaacg cgatgctcgc cttccagcag
     8881 tggtacgact tcgacgactg cggcggcgat cccaccttcg agcccgacgg cgcgatcgtc
     8941 gaggtctcga ccgacggcgg cggcacctgg acccaggtgg ctccgatggg gggctacccg
     9001 tacgtgctgg acgacatctg cggcaacccg ctcgcgaacc ggccggccta ttcacacacg
     9061 ggtggcgtgg gctccgcctt catacccgcc gtcttcgacc tgaccccgtt cgccgggcag
     9121 atcctccaga tccgtttccg cgccggttgg gattgcggca actgcgccgc gaacgaaggc
     9181 tggtacctcg acgacgtgtc cgtgtactcc acgatgccgc ggatcgtctc cgtcagtccc
     9241 gtcgagggca cgatcccggc cggaggggcg ctcgggctct cgctcgcctt cgacgcctcg
     9301 ggactgttcg gcggcgacta cacgaacgac gtcctggtgc gcagcaacga tcccgacgag
     9361 aacccggccg tcatcgcgac gtcgctgcat gtgaccggcg cgccggacgt ggcctgggat
     9421 ccgccggccg tggagttcgg cgctcgcttc atcggagggg cggcgacgga cacgctggcg
     9481 gtgttcaacg cgggcaccga cgtcctgcac gtcaccgagg tcacgctgga ccggcccgaa
     9541 taccaggtgg aaaccgcccc cttcaccctg gcgcccgggg cgcgccacga cctgcacgtc
     9601 acgttccggc cggtggcgac cggattcctc acggcctcgt tgatcctgcg aagcgacgat
     9661 cccgacgagc cgaacgccgc cgtttcgatc cgcggcgagg gcgtggtccc gcccagcgcc
     9721 ggccttgcgc ctgcctcgat cgcggcgacc gtggagacgg ggcagaccgc cacccgcacc
     9781 ctgaccttgc gcaacgacgg cggcgcggac ctggtgttcc aggcgcacgg cgacgatccg
     9841 gcctccgctc ctcccgccga cagctcggtg gcgatccccg attcgctccg cgggccgacg
     9901 gcgaacacgc tggccccgac gccgggcgag ccccaggtcc aggccgtcta cgccggcacc
     9961 tatcttcggt tcggcgtcac cagcttcggc gagatcatgc cgtaccagta cccgctcggg
    10021 aacgagcacc tgcaggtggg gacctacgtc tctggataca cgctcgcgta ccagtcgggc
    10081 ggcgtggacc acatcgcctt cgccttcgta tccgaccatt acggtgtcgc cccggagcag
    10141 taccgggagg tcgagaattc gcccgcccgc gtggtcgtgg aggtgtccgt gcgcaccgcg
    10201 gatggattgc tgcgggtcgt gcgcacgatc acgttcgagc gcgcggcccg tgccgtgcgc
    10261 gtcgacactc gactggagaa cgtcgcgggc acgacgctcg gggccgtggt cttcaaggag
    10321 gaagcggact gggacgtgga ccagaactac aacgacgact gggactatga ccgcgggcgc
    10381 catctgatct acgcgaagtc gatccacgtc gcgggcctgg cctcggcgga agcgccggac
    10441 ctgatggaca tctacggctg gaacgatctc acgtcgcgct ccacgttcgt ggacttcccg
    10501 gcggcaccgg tgctctcctt cgacgggctc gcgctcctcc atttcgacct cggaccgatg
    10561 gctccatccg acgcccggac gatcgcggtc gcctacggtg ccgccgacga cgtgaaccag
    10621 cttcgagcgg ccctgtcgga cgcgctccgg gggctgacct ggctgagcgt cacccccgcg
    10681 agcggcaccg tgccggcggg cggcagcatc gatctcacgg cccgattcga tcccggccgc
    10741 cgttcgagcg gcgactacga cgggcgggtc gtggtggaca gcaacgaccc ggtccatccg
    10801 cgactcctgg cttccgccgt catgacggtc atcggccggc cgctctttac ggcggttccg
    10861 gccgcgctcg ccttcgactc gatcttcgtc ggggccacgc tgagggactc gctgcgcgta
    10921 cggaacggcg gcgtcgatcc gctgctcgtg agcggcgtga cgtcgcccga tccgtcctat
    10981 gcggcctcgc catcggcctt cgccgtcgcg cccggcgact cccagctcgt ggtcgtcacg
    11041 ttccacccgc tcacggcggg ggaccggacc tccacgctgc agttcgccca caacgcggcg
    11101 gggtcgccct cctccgtcgg ggtttcgggc ttcgccctcg ttcctcccga catcgccgcg
    11161 gatccggcct cgttcacggt cgacaccgag acggggcgcg tggaaacgcg aacgctcacg
    11221 gtccggaacg acgggggaag cgagctggtg ttccaggcct ccacgctcgg actgagcgag
    11281 gcggcgcttc ccggcacgcc cgtgtcgctg cccgacagtc tccaggggcg cgccacgccg
    11341 aacacgaccg cgccgtcggc gccctccgcg gtccaggcga tctacgaggg cacctacctg
    11401 cgcttcggca tcacgtccta cggcgagctg atgccctatc agtacccgct cggtgtggag
    11461 cacctggagc tgggagcgac ggtttcggga tacacggtcg cgtatcggaa tggaggtcac
    11521 gaccacctgg cctactcggt cgacttcgaa cggtccaacg tcgtggcgga gagctacgcc
    11581 acgctcgaga actcgccgca gcgcgtgatc gtcctcgtca cgaccaagac ccaggatggg
    11641 atcgttcgga tccggcgccg gttcgacttc gagcgctcgc gacggggcgt tcgaatcgag
    11701 accgccatcg agaacctgac cgccacctcg ttgaccgacg tcgtgttcaa ggaagtggcc
    11761 gattgggaca tggacggcga attcaacaac gtctggaact acgatcccgc gaacgcgatg
    11821 gtgtacgcgt cgcggaccca ctacggcgcc atcgggtcca agcagccggc cacgatcatg
    11881 gatctctacg gctggggcga ctactcctcg cgctccacgt tcgtggacat cccgtccggc
    11941 ccggtgtcga cggatggcct cgcgctgctc cacttcgctc tcggggacgt gcctcccgcg
    12001 gaggcgacca gtacggtgat cacggcgtac gccatggggg gcgatctggg ccagcttcgg
    12061 gaatcgatgg ccctcgccgt cggatcggcc tcctggctgg ccgtgtcccc caagacgggg
    12121 cgcgtaccgg ccggcggggc gatggatctg acggtccggt tcgatccgga acgccggccc
    12181 accggcgagg tccggggcga gatctggctc gagagcaacg accccgacga ggtcctcgtg
    12241 cagttgcccg cgacgatgcg ggtcaccgca cggccggtga tcgcggtgac ccccgcggcc
    12301 ctcgacttcg ggatcgtgtt cctgaatgaa tcggcgaccc gcaccttccg ggtccggaac
    12361 gacggcgtcg tgccgctgca cgtgacctcg atcggcgcgt cggacccgtt ctacgccgcg
    12421 actccggcga ccctgaccgt ggcgccgctc gattcgcagc aggtcacggt gacgttcacg
    12481 ccggtcacgg tggggctgcg caacgcgtcg ctcgccctga cccacgatgc ggccggctcg
    12541 ccgacgcccg tggccctgac cgggaccggc gtcgtgcctc cgacgatctc cttcctcccc
    12601 gcgagcgtcg aagcggccgt ccgttccggc cagggtggga cgacgaccct gagcgtgtcg
    12661 aacgccgggg ccgccccgct caccttcacg atcgcggcgc cgcctccgtt cccctccgcg
    12721 ttccaggcga tgagctatca ggagctgcgg aagggggaga aggacgcgcg acccggaatc
    12781 ctaggcaccg gaggggccga ccgcttcggg cacttctgga tcgacagcga ccagccgggc
    12841 gggcccgcct acgaatggag cgacgtgcgc ggcatcggga cgcacgtgcc gttcgcgccg
    12901 gtgaccgacg acgcgacgtt gggaccgttc cccatcgggt tcgacttccc gtggttcgga
    12961 ggcgtggtcc acgagttccg tgtctgcagc aacgggtgga tcagcttcac caaccccctc
    13021 gcggcgttcg ggaatcagcc gctgcccaac gcaggaccgg gcgtgcccga gaacctgatc
    13081 gccgcgctgt gggacgacct cctgctggac gcctcggcgg gatccagcgt ggacgtctgg
    13141 ggcgacaccg agcacctcgt cgtccagtac accaacgtgc gcccgctggg ggtcctgtcg
    13201 ccgacgccgc acacgttcca gatcgtgctc cgccgttcgg gcgagatccg cctcttctat
    13261 cagagcgtgg gctcgctcct ctccggtgcg acggtgggca ttcagaatgg aacgcgagac
    13321 gacggcctca cggtctcgtt caacaccccc tacctgcacg accagctggc cgtgcggatc
    13381 gtgaagcccg ccccctggct catcgtgacg cccatgaccg gatcggtcct gcccgcgagc
    13441 tcgctgccgc tctcgctcca gttcgatccc cgcgagctca ccgacgggga tcacgaagcc
    13501 gacttgaacg tggcgagcaa cgaccccgcc catcgttcgg tctcggttcc cgtgctcctg
    13561 cacgtcggcg agacgccggc cacggcgtcg gacttcgatc cgaacacggt gaacaaggag
    13621 agcaacggcc ggtacgcggt catgcgcgcc gagctcccgc ccggcgtcga cccgtggaag
    13681 gtcgcgatcg agaccgttcg attccaggag acgctcgcgc cggccgagga ctcgctcgcg
    13741 atcgaggact ggaacgcgaa cgggatcccc gatctacgct tccgcttcca ccgcgatcag
    13801 gtggagggga ttctccccga gggagaacgg gttccggtcg tgctgacggg ccgcgtcggc
    13861 aaccagacct ggttcatcgc gcgcgattcc atccgcgtga tccggccgcg cgtcctcgcg
    13921 ccgaacgggc aggaggtgct ggtgccgaac acgacgtaca ccgtctcgtg gaacaacccg
    13981 cagctcggcg cgcccgacga agccgcgctc tactactcga ccgaccggga ctcctcgtgg
    14041 aacctgatcg ccgagcacgt cacgggcacg tcgtaccgct ggctcgtgcc cgccgtgtcg
    14101 gccccctggg cgcgcctgcg cgtctacctg ttcgacaagc agggcgtcat gggctacgac
    14161 gcgagcgatc agtccttcgc catcgtcgcg agcccgaccg gggtgaccgt ggaggagacc
    14221 gctccgccgg cgtcgttcgc gctccggcag aaccggccga atcccttcaa tccatcgacc
    14281 gagatcggat tcgacctgcc gctggccgcg cgcgtgcggc tccagatctt ccagccgaac
    14341 ggccggctgg tccgcacgct ggtcgacgag ccgctttcgg cgggccgcta tcgcgcgccc
    14401 tgggacggcc gcgacgatcg gggccgggcc atggcgtccg gggtttacct gtaccggatt
    14461 tcagcgggtc cgagcacgga gagcaggcgg atgctgctgc tcaagtgatc gcatagcggc
    14521 gatcgtgctg ccgaggcggc catccctttc ggggtggccg cctttcgtat gacccctcaa
    14581 gggcgtggga agaccgtgaa gccggtccaa tccatggcgg cggagccgaa gatgccgatg
    14641 ccgccgttga catggaagat cggcctctgg aattcgtccc ctgcgttact gccgaagccg
    14701 aggccggcgg gcgcgtagaa gttgatcgac cgggccaggt cgtaccagtt gcgatccacc
    14761 gaatagacca cgaagcggtg gcgaccttcg taccaaaccg cgagccatgg gagtcgaacg
    14821 aatccatcgg gcgcgtccag aggcggcgag gcgctctggc gctcgaggct agccttgtcg
    14881 gcctcgctca gaaaatcggc gtcgatcaac agaggcgagt tgtcgtccag gctgaaaagc
    14941 gccatctgaa acgccatcga tccgtcgcgg tcaaagcgag cttcgacaag cccgtcctgg
    15001 tagacaagtc ggttggagct gtacacgctg tcgtacgcga gcacctcttc agggaattcc
    15061 ggcacggcga gacgccgcct cacctcgagc gtcctgtcgt ccaccaggag ccattcgcga
    15121 acgtggaacg tgtccggcgt cgtggtttcc gcggtgacga cccgcccgtc cggtgcccgg
    15181 acatggagcc ggtacgttgt gagaggctca actcgggcga cccctggtgt gtagccgacc
    15241 ggctgatacg gatagattcc actgtctgaa tatcggatcg tgtctcctcc catcgtcgtc
    15301 aggatgacgc ttgcgtccct caggcgcgcc gaagccacct catagggacg gtcgggcgcg
    15361 acggtcgtgc cgagccagat cggcggaaag ccttccccca cgatcaagcg cgcgtccacg
    15421 acaatggtgc cgcccccatc aggtccaagc agggatctcg ggtcacgctc gcccgagcac
    15481 cccgctccga ggatgagggc gcacgtaagc accgcgagaa ggggtctcat cggtcgaagt
    15541 tgatcccgat cgtcgggacg agtgggagct gatgcacgat cttgggctcg gtttcgggat
    15601 tatccgtgtc gtactggacg aaccattcgt tctttcggtc ataggcgttg aagacctgta
    15661 catagtagtc gacgggcaca ccgaacaggt gcccgtgccg cgtgacactg atgtccagtc
    15721 ggtggtaggg aagcaaccgc gaactgttcc gatcacccgg aagaaccaga ccgtcgtgca
    15781 ggcgttccgt agcgggatcg ttcaagccat atcgcgactc caaaggcgtg aaggcctggc
    15841 cggtcgcgaa gacgaagtcg gcgccgaagc tccagcggct gcggttccac tgcgccacca
    15901 ccttcacgtc gtggcgtcgg tcgtacttgg gcgggaaggt ctgcccttcg ttcagctccg
    15961 gccagcggcg gcggctccag cccagcgtgt agccgaccca gccggtcagc gcgccgcggc
    16021 gcttctgcgc gaacagctcg acgccgctcg cccagccgcg gccgccggtg tagaagaggt
    16081 cctcggtgct cgtgccctgc gcgtcggccg aacgggtgtt gtcgagctgc acgagattcc
    16141 gaagccacgt gtagtagccc tcgacggaga cggtgtaggc atcggtcgga agccactcca
    16201 ggccgctcac cgcctgccag gagcggcccg gcttggccga gccgtccgtc ggaacccaga
    16261 aatccgtccc attgaacgcc tccgtcgaga cgagctgaag atgctgcgtg tagcccccgc
    16321 cgcccgcctt cacgcgcacg ttcgggccca gcacgtggtt cagcgagagc cgcggctcca
    16381 cgcggacatg gccgcggggg ccgaacacct cggcgcgcac ccccggccgc accgtccaga
    16441 ggacgttcgg cgtccattgg tcctcgacga acgcggccgt cccgaccggg cgctcgtcga
    16501 atcccggctg ctccacgccg ttgaactgct ggcccagctc gaaccggtac gccgaaaccg
    16561 aaacgccggt tcgtgcggcg tggccggtcg ccggattcca ggagaggtcg cccttggcgc
    16621 tcacgtcgag aagccggttt ccgaagaaga gcggcgtgct gaagatcttc acgtcggtgt
    16681 cgctcttgta ctcggtggcc gaaacgatca ggttcccgat cgcgccgcgg ccgaaatcgt
    16741 gcatccagcg ccccatcccc gccacgttgc cccatcggat gtcgacgaac gtccctcgat
    16801 cgagatcgag atgcagcttg tcgcgcccgc gatacccgga gaacgagagg tcgtcgcgcg
    16861 ggctcatcga gcggtgcacg cggccgttca gatcgtagaa gaagtacgag gggatctcgg
    16921 tcgaatcgtt gcgcaccgcg ttcagcaccg gctcgaggta ggtccgccgc gccgagagga
    16981 tccaggatcc gttccggatc ggcccctcga ccgtcgcgcg cccggcgatg aggctcaccg
    17041 cgccggcgcc gcgcaggccg tcgcggctgc cgtcgcgatt ctccacgtcc agcaccgagc
    17101 ccagccgccc gccgtactgc gaggggtagg cgcccttgta cagggcgacg tccttgatcg
    17161 cggcgggatt gaacgtcgag aagaatccga acgcgtgggt cgggttgtag agcgggatct
    17221 gatcgagaag gatgagggtc tggtcgggcc caccgccccg gatgtagagc ccgctgctga
    17281 agtcggacgc cgattgcacg ccggggagaa gctgtaggct ccggatcagg tcggcctcgc
    17341 cgacggccgg cagccgtttg agctcgcgcg cgggcagctc gacgtagctc ggcgttccgc
    17401 gcacgtcccg cggctcacgc tccccgcgca catccacggc gctcacggga aagggcgacg
    17461 tcgccagcac gatgtcgcgc cgaagggtgc cctcgccgat cgcgacggtg tcggcgaagt
    17521 cgcggtaccc cgcggcgcgg caggtccggc ggtactcgcc gggggttatg ccgccgaacg
    17581 aatagaatcc ggtcggattg gatacggcgc gcaagccggc ccgtgccgcg gccgcggagt
    17641 cgccggcagc cagaggagtg agcgcgatat aggcgtaggc ggggccttcc gcctccccct
    17701 tcgcgcggac gaacccctcc agcgatcccg cggtcgcgat cgtgccgccg aggaccagat
    17761 cgagtaggag acacgccaca gccgccatgg gccg
//
LOCUS       DASSLZ010000063        17149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1545235, whole genome shotgun sequence.
ACCESSION   DASSLZ010000063 DASSLZ010000000
VERSION     DASSLZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 17149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17149
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545235"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="VFM00_02125"
     CDS             complement(<1..1571)
                     /locus_tag="VFM00_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7497073.1"
                     /translation="MRLAFLDLPDDRVDLISTARGADGVELVLVASADPESLSLRIAE
                     VLQIPRSTEPLDLLALKPDRVALPSLESPGASALLRAGISPNIFLPLPDLLAILNGGA
                     SERAPDPPPAPIDEWEREFEVAAGAGHRLTEIRDALALSDDRQRLFREVLALSIAETG
                     ADAGSLMLVDEDEGELRIAFADGLSHDTVRTVRQKIGEGIAGTVAQSGKPLIVNERVL
                     DPRYKDRERPRIASAMSAPIQLDERVIGVLNVSSNRQGKRFKDEDLDRLVEIGAQISA
                     ILDRVVRRSRSDLEALEFRARQGLDRVFKAENGPRIERLRAAATRLAMRLGAQSVTVY
                     AAEAGAREFAVLASGSGAGETGTLPVHAAGLVGRAHREGESACLTGEVVRPGEEGMTR
                     PAVLLVTPLRGREVHGVLAAEFAGAGDYDPEALAHVLDHVAAYLVSLLERRKPDQGAE
                     RRGAMVSQLADIAPRLMVTREMEGLALETIAALRALFPSGLAVARLLDKNGDVVLRTA
                     YEGAEEGREPSCGREA"
     gene            complement(1732..3804)
                     /locus_tag="VFM00_02130"
     CDS             complement(1732..3804)
                     /locus_tag="VFM00_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497074.1"
                     /translation="MSTSFWRLDSAGDFLQGEQVVGVAIDSDGYLTLGPGWDSVVTHL
                     PDVAYIWSLARDTKGRVYFGTGDNGGIYRWTRGEGPKLLWKTGASEITAMAVDAKDDV
                     YAASAPGGEIFRITARGDTTRYFKTGEESIWSLLVGKDGALYAGTGPRGKIFQVTGPG
                     KGSVYAETKDVNVLALAWAKDGALLAGTGAKGLLVKVDGAGRQRVIYDADADEIRAIA
                     VLPDGSVAVGASKGQASSRATGSGSNPYAVDVTPTSGGRSGVYLVQPDGSARLLYAPS
                     ADYIYAMIPADDQSVWVSTGDPGALFRVSTNRKYALLGAPDEKQVLALVRAGGETFAA
                     TGNPATLYALGSGPAAQGTYLSDAHDLRSVASWGRALISLRDGEATWSSRSGLSQTPD
                     DGWSGWSPETPIGPGGAEIKSPPARFLQFRLRLKKAGGDLPLLSMAEVSYRQRNLPPE
                     IANIQVFGPDQPFMEGGPEYRPPQISQTFPNGLKVEYSLPKTGPRAVSDASAAWARGV
                     RTVTWDAADPNGDDLTYDVLIRSDDEKEWRRLASDKKDRVVSWDAESYANGTYRIKVI
                     ASDKMDNPPDVALSSERTGPAVTIDNVPPRIEGLKTSSGSGLGGRGVVVSGTAIDADS
                     RIAVIEYSVDGGDWVQIFPEDGLYDQRTESFRIPVPSLPPGEHTVTVRASDQDRNVAI
                     AKVLTVAK"
     gene            complement(3938..5743)
                     /locus_tag="VFM00_02135"
     CDS             complement(3938..5743)
                     /locus_tag="VFM00_02135"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HET7497075.1"
                     /translation="MTTRRALGRVLPALLLASGILGVLLGGTLPGAAAGAALLPLIPP
                     SATLPVSEVREGMTGTGYSVFSGTQIDTFAVTIVGVLKGYRPGFDLIMAKASGPVVDR
                     TGVIAGMSGSPVYIGGKLIGAVSYTWSFLKEPMAGITPIDQMLSLLPAPGAKPPNAED
                     AFGSLGAPKEQSADAGGARPIATPLALAGFTNEAIHYLEPWLSERGFVASPGGANAPG
                     GSCDSLRPGSAIGVQLVRGDWSAAAIGTVTYRDGDRVLAFGHPFLAMGWMELPMTAAK
                     IHTIMSSQQISNKIGSPTVTCGTIFADRSVGIAGTMGPAPSMIPVSVAIAGSGGRSRS
                     YHMEVARSRYLTPALVSATVVNSISEALYDAGVSTLRYEFALYMNGGGRTIHKEDTFI
                     STAPVSGVGETVGQSLTLLLGDRFRPSRLDSVRVDVRVEDGIDEAALLGIRVAPAAII
                     AGDSLDVDLTLRRSNRLVETRRVRVRVPPATPAGDLTVRVCDATETEKWEMQRAPERY
                     QPETFDQLADLIESDRPSDKIYVQLYRTVGGAVVGGREMSQAPGSVLNVLGSSPKSGE
                     SATTKGATVSELAIPLGHVVRGCESATVTVIPGVR"
     gene            complement(5781..8447)
                     /gene="mutS"
                     /locus_tag="VFM00_02140"
     CDS             complement(5781..8447)
                     /gene="mutS"
                     /locus_tag="VFM00_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET7497076.1"
                     /translation="MEATPARMEANAAAEAVSARGETTPMLEQYRRLKAEHPGSILLF
                     RMGDFYETFGEDALIASRVLGIALTSRDKKRDPLPLAGVPHHSVEGYLKKLVERGYSV
                     AIAEQMESAEEAKGLVDRQVVEVLTPGTVTRLGLLEATESHYIVALAPGEARVGVAVA
                     EVSTGEFRAGTVDAEGLAALPLTFPAREVLMPLPGRGARDPVCGEGESGPYGKTPITR
                     RETARFDPEAGRQALLRHFSVARLDSFGLDAGREGLGAAGALLDYLRSLAKSDLPQIR
                     EIRPLREGEPLVVDEVTLRNLEVFESPAGKEHSLLHLLDRTETSMGARALRSLLRKPA
                     RETAVVAARLDRTACFAESAPLRTELAERLHRFPDLERTLGLLGSGRAAPRDLGSVRD
                     ALRRLPAVRLTLEARTGAAIGAWRESLPDLSGLAALLEEALMEELPLTAAQGGIIRPG
                     FDPDLDAMRSDAGDGREQVLALEARERARSGIANLKVGYNRVFGYYIEVTRSQLNRVP
                     EDYIRKQTLAGAERYVTPELKRMEERIEAASVESRRSEAAHFHRLRERCLESIGALHD
                     AARVVAELDLYRALGETAARERWTRPALTEDRKLVLKQSRHPMVERALDGGAFVPNDC
                     ALDGEREQIWLITGPNMGGKSTFLRQVGLAVYLAQVGSYVPAAAAEIGLADRIFTRVG
                     ASDQIARGASTFFVEMEETATILRQATDRSLVLLDEVGRGTSTYDGLSLAWAVTEALH
                     QGPRARPRTLFATHYHELTDLEATLPRLKNRNVRVSENEGKIVFLHVIAPGRADRSYG
                     IHVAQLAGVPGEVLARAEEILARLEREHAAGQNGARREGTGGAARGVGADERAALLAE
                     LADAPVERLTPLDALQRLASLRDRARGSGGTR"
     gene            complement(8454..9515)
                     /locus_tag="VFM00_02145"
     CDS             complement(8454..9515)
                     /locus_tag="VFM00_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7497077.1"
                     /translation="MRHGLRAAALVLLLGGAVTLVAAAPRKEAKKPSGPEANAALLVA
                     ARDAFDRGRTEEARAILIRIPENGLTPDKADAAAYLRALVLEDGNAYDEALSDYLAHY
                     PKGVFRREATLALARLRYVSGDYAQAENLLAIFTPGVERSATGRQGLVTRGLAQLGRG
                     DAAGALQFLASAESDLKGSPEEESYEYATAEAALRASKPSAAMAALRTLLERHPQGDY
                     APQALYAMGTALEAVGRQADAASVFRQVAQRFPLSYEAARVRDRGIRPGDTVTPLLPI
                     GGGYAIQIGAFTRRDLADALARELRAAGNDDVSVRQGSETPPLFRVRAGSFATRDEAR
                     ALGERLRRERGLSYQVVPR"
     gene            complement(9512..10651)
                     /locus_tag="VFM00_02150"
     CDS             complement(9512..10651)
                     /locus_tag="VFM00_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497078.1"
                     /translation="MAPLFTWVAVVGALAAVGYAAWLFSRDANTGPPRPETPYQRGVH
                     ALLAGDKDAALEAFADTVQEDSGNIDAYIHLGNLLRERGEAARALDLHRKLTARAART
                     PSQDKAIREALVLDWIAVGRPKEAIAEASELRERERKNGSGLPLLLQAYEAGGEWDRA
                     YDVRVEMAKARGEKETAALARYRAAIGEVYLRNEHLEEAAKHFKAALKLERDLPVALL
                     RMGDIYYETNHPDRAMALWEGLAAAHPEHSHLVLERLEVSYFERGRFGDLAPLYEQML
                     QKNPRDARILLALARMHMKRGDLSEATHAAREAMTVDPHSLEARLLVIEIHRRLGDPA
                     RALTEVDTLLRELGGSAPPACPKCGERAEEYWSRCPSCLAWLERA"
     gene            complement(10677..11003)
                     /locus_tag="VFM00_02155"
     CDS             complement(10677..11003)
                     /locus_tag="VFM00_02155"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HET7497079.1"
                     /translation="MWFLRNVLWLAVMILVVGFAILNMRESVTAINLPGHVYRSIPVN
                     VAVFAAFAAGMFLAFLLVLFQVLKARSEVAAAERQKQELERELAELRNLPLEGLALGS
                     KAGGGR"
     gene            complement(11084..12460)
                     /gene="mtaB"
                     /locus_tag="VFM00_02160"
     CDS             complement(11084..12460)
                     /gene="mtaB"
                     /locus_tag="VFM00_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HET7497080.1"
                     /translation="MPYEIRPGASATAPRVFYFTLGCRLNQHDTAAMRAALGGAGWAE
                     AREGEAADVVVVNTCTVTARADQEARQAIRRLAREHPGARVVVTGCYAQRAPREVAAI
                     PGVSDVMGIAERETIAERLGAAGAVVPRDPIMQRNDLAVIAVALARAKRPPAPAGDSL
                     PIPLAFGRTRALLKVQDGCDAFCSYCVVPYVRGRSRSLPLAEATARARRLLDAGFHEI
                     VLTGADLGAYGRDLGRRGLLAALVESILSLGPSHRVRISSIEPNKVDPSLAALAGSEP
                     RLCRHFHLPLQSGSPAVLRAMRRPYRPEDYARLVERLAGGGPAAIGADVIVGHPGEGE
                     AEFEETCRFVSLLPITHLHVFRYSPRPGTRASVQSDAASLAEARERSERLRALGDAKR
                     DAFYRSLIGSVRPAIAERGQLSEPACIAMTDIYAPVRLERRPAAGGILPVRIEALRNG
                     ALAGAAIG"
     gene            complement(12466..14334)
                     /locus_tag="VFM00_02165"
     CDS             complement(12466..14334)
                     /locus_tag="VFM00_02165"
                     /inference="COORDINATES: protein motif:HMM:NF004953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET7497081.1"
                     /translation="MVRRHEEELEVKDDGADEAEAEAKPRKRRTTTRLIHQDDDEELF
                     TSPGAAVGAVEVDGEDEAGAMGQWLQLYEESLQDLEEGEIVRGRVLKIDDKEVTVDVG
                     FKSEGVIPVEEFPDLEGVKVGDEIEVFLEKTENQDGLVVLSKQRADFVKVWDRVKAAA
                     DQGELVEGRLMRKIKGGVVVDLYGVEAFLPGSQIALRQVQNVDSLLGQSMEFKIIKLN
                     KRRRNIVVSRRAVLEEHRAKQKSEIIQELAKDQIREGVVKNITDFGAFVDLGGIDGLL
                     HITDMSWGRVSHPSELVSIGDKVKVKVLSFDPEKERISLGMKQLTAYPWEDVDKRYNV
                     TQRVKGKVVSITDYGAFVELEKGIEGLIHISEMSWTRHVRHPSKVVAIGDEVEAMILK
                     IDKDNEKISLGLKQIEPDPWLTLDDRFPIGTKLSGKVRNLTNFGAFVEIEEGIDGLVH
                     ISDMSWTRRVAHPSEILKKGDKVDVVVLSIDKDARRISLGLKQVSDDPWPTLAERYLP
                     DIIVKGTVVRLLDRGVIVDLEDGLEGFIPLSQLGVDGLKKPSDSFKPGDTLDLKVTRV
                     DTQAHRIILSAKAWVADQDRAGQEAFQERFKPSPSAAEETGEPAPVAEGSDDTTAA"
     gene            complement(14401..15261)
                     /gene="ispH"
                     /locus_tag="VFM00_02170"
     CDS             complement(14401..15261)
                     /gene="ispH"
                     /locus_tag="VFM00_02170"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET7497082.1"
                     /translation="MEVVVADNAGFCFGVKRAIKMATDTLDEAQGPVKSLGQLIHNPQ
                     VVSLFHRRGLEVVSDLTELAPDGTVVIRSHGVGPEVHEQATSRGLKVVDTTCPFVTKA
                     QQYAAKLIAENYKVVMIGDAHHPEVIGVVAHTQGKAIVIDTVEEAEKLKFIPRMGVVF
                     QTTHSIGHVQEVVGALLKRGKEVRVFNTLCGATTSMQKTAVELAPSVDAMVIIGGRQS
                     ANTAQLAEVCRKVNPRVHQVESAEEIDPSWFTGVRRVGVSAGASTPDEVIAEVVARLT
                     DLEPGQARSA"
     gene            complement(15279..16544)
                     /gene="cmk"
                     /locus_tag="VFM00_02175"
     CDS             complement(15279..16544)
                     /gene="cmk"
                     /locus_tag="VFM00_02175"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET7497083.1"
                     /translation="MRAFVVAIDGPAGAGKSATARAVARALALPHVDSGAMYRAAAWV
                     ALQAGADLDSEAEMLRSLEGVRFEPTPEGLRCGGRLLDGEIRTAEAGDAASRVAVHPG
                     LRRRLVAIQRSFASPPGVVMEGRDIGTVVFPRADLKIFLEASVAVRALRRFRELESRG
                     ERPDLTAIEAMIRERDARDQGRAESPLLPAGDAIRLDTSDLGLEEQVAVVTRWAELAR
                     RGPGPTKPFYWFGSRFVKRFSRTFLKFRIEGLDHVPRSGPLIAACNHISFWDPPLVGS
                     NFPRVLHFVAKAELFQNKVFGAMLRGYNSIPIQRGTRARSGLLGAEEVLNGGGAVLIF
                     PEGTRNKSGTLLAPRAGIGRLAVVTRSPVLPIRISGSNQIRRSLRRQTLVRIVVGTPM
                     TPPIRVDADREAMNSFAAAVMEAIAALPG"
     gene            complement(16541..>17149)
                     /locus_tag="VFM00_02180"
     CDS             complement(16541..>17149)
                     /locus_tag="VFM00_02180"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET7497084.1"
                     /translation="EIEVPGDLSAAAFLVAAAALVPRSEITIRGVGVNPTRTAFLDLL
                     MRMGAGIERLEARGAGEEPLADLHVKAGGLRPVTVADDEAPRLIDELPMVAVLAAFAK
                     GRSEIRGARELRVKESDRIATVAAALRAAGGAVEELEDGWIIEGKGSLRGGRVDAAGD
                     HRVAMAFLVAGLVAKEGVTVEGADAAAISDPDFLDRLRSLRR"
BASE COUNT         2427 a         6181 c         6009 g         2532 t
ORIGIN      
        1 gcttcgcgcc cgcacgacgg ctcgcgtccc tcctcagcgc cctcataggc cgtgcgcagc
       61 acgacgtcgc cgttcttatc gaggagccgc gcgacggcga gcccgctggg gaagagcgcg
      121 cgcagcgccg cgatcgtctc cagcgccagc ccctccatct cgcgcgtgac catgaggcgg
      181 ggcgcgatgt ccgcgagctg cgagaccatc gccccccgcc gttcggcgcc ctggtcgggc
      241 ttccggcgct cgaggagcga gaccaggtag gcggcgacgt ggtcgagcac gtgcgcgagc
      301 gcctcaggat cgtaatcgcc ggcgcccgcg aattcggccg cgagcacgcc gtgcacctcc
      361 cggccgcgta gcggtgtgac cagcagcacg gcgggacgag tcatcccctc ttcaccgggg
      421 cggacgacct cgccggtgag gcaggcgctc tcgccttcgc ggtgggcgcg tcccacgagg
      481 ccggccgcgt ggaccggcag cgtgcccgtc tcccccgcgc ccgatcccga ggcgagcacc
      541 gcgaactcgc gcgcccccgc ctcagcggcg tagaccgtca ccgactgcgc gccgagccgc
      601 atcgcgaggc gggtggccgc cgcgcggaga cgttcgatgc gcggcccgtt ctccgccttg
      661 aagacacggt ccagcccctg gcgcgcgcgg aactccagcg cctcgaggtc gctccgcgaa
      721 cggcgcacca cccggtcgag gatcgcggag atctgggcgc cgatctcgac cagccggtcg
      781 aggtcctcgt ccttgaagcg cttcccctgg cggttgctcg agacgttgag cacgccgatc
      841 acgcgctcgt cgagctgaat gggcgccgac atggccgacg cgatgcgcgg gcgctcccgg
      901 tccttgtagc gaggatcgag aacccgctcg ttcacgatga gcggcttccc gctctgggcg
      961 accgtgccgg cgatcccttc tccgatcttc tgccgcacgg tgcggaccgt gtcgtgcgag
     1021 agcccgtcgg cgaacgcgat gcggagctcc ccttcgtcct cgtccaccag catgagcgag
     1081 ccggcgtcgg cgccggtctc cgcgatcgag agggcgagca cctcgcggaa gagccgctgg
     1141 cggtcgtcgg agagggcgag cgcgtcgcgg atctcggtga ggcggtgtcc cgctccggcc
     1201 gccacctcga attcgcgctc ccattcgtcg atcggggcgg gcggcgggtc cggcgcgcgc
     1261 tccgaggctc cgccgttcag gatggccagg aggtcgggca ggggcaggaa gatgttcggc
     1321 gagatcccgg cgcgcaggag cgccgaagcc cccggcgact ccagggacgg cagggcgacg
     1381 cgatcgggct tcagcgcgag gagatcgagc ggctcggtgg agcgcgggat ctggagcacc
     1441 tcggcgatgc gcagcgagag ggactcggga tcggcgctcg cgacgaggac cagctccacc
     1501 ccgtccgcgc ccctcgcggt cgaaatgagg tccacccggt cgtcgggcag gtccaggaaa
     1561 gcgagtctca ttggatttag ttgtgccggc cccgtttggt aggcggaatg gtccgaggcc
     1621 gagaacgcaa agagtgtacc accctcgaaa ccgcgtgggg gcgcgccggg gccgcgcggg
     1681 cgcattgcgg catgcgcgtc gggatgcaag ttgcacggct cccgggaggg actacttcgc
     1741 tacggtcagc accttcgcga tcgccacgtt ccggtcctgg tcggacgcgc ggacggtgac
     1801 ggtgtgctcc ccgggaggaa ggctcggcac cgggatccgg aagctctccg tgcgctggtc
     1861 gtacaggccg tcctcgggaa agatctggac ccagtcgccg ccgtcgaccg aatactcgat
     1921 cacggcgatc cgcgagtcgg cgtcgatcgc cgtgccgctc accacgacgc cgcgcccgcc
     1981 gagcccgctc cccgaggagg tcttcaaccc ctcgatgcgc ggcggcacgt tgtcgatcgt
     2041 cacggccggg ccggtccgct ccgaggagag cgccacgtcc ggcggattgt ccatcttgtc
     2101 cgaggcgatc accttgatgc ggtaggtccc gttcgcatag gactcggcgt cccacgagac
     2161 gacgcgatcc ttcttgtcgc tcgccagcct ccgccattcc ttctcgtcgt cgcttcggat
     2221 cagcacgtcg tacgtgaggt cgtcgccgtt gggatcggcg gcgtcccagg tcacggtgcg
     2281 cacgccgcgc gcccacgccg cgctcgcgtc ggagacggcg cgcggcccgg tcttcggcag
     2341 cgagtactcc accttcaatc cgttcgggaa ggtctgcgag atctgcggcg gccggtattc
     2401 cgggccgccc tccatgaacg gctggtcggg tccgaacacc tggatgttcg cgatctcggg
     2461 cggcaggttg cgctgccggt aggagacctc ggccatcgag aggagcggaa gatcgccccc
     2521 cgccttcttc aggcgcaggc ggaactggag gaagcgcgcg ggcgggctct tgatctcagc
     2581 gccgccggga ccgatcggcg tctcggggct ccagccgctc cagccgtcgt cgggcgtctg
     2641 gctcaaaccg ctgcgcgagg accacgtcgc ctcgccgtcg cgcagggaga tgagcgcgcg
     2701 cccccaggac gccacggaac ggagatcgtg cgcgtcggag aggtaggtcc cctgggccgc
     2761 ggggccggag cccagcgcgt agagggtggc cgggttgccc gtcgccgcga aggtctcgcc
     2821 gccggcccgc accagcgcga gcacctgctt ttcgtccggg gcgccgagca gcgcgtactt
     2881 gcgattggtc gagacgcgga agagcgcgcc ggggtcgccc gtcgagaccc acaccgactg
     2941 gtcgtcggcg ggaatcatgg cgtagatgta gtcggccgac ggagcgtaga ggagccgcgc
     3001 gctcccgtcg ggctgcacga gatagacgcc gctccgtccc ccgctcgtgg gtgtgacgtc
     3061 cacggcgtag ggattggaac cgctccccgt cgcgcgcgac gacgcctgcc ccttgctcgc
     3121 gccgaccgcg acggagccgt cggggagcac cgcgatggcc cggatctcgt cggcatcggc
     3181 gtcatagatc acgcgctggc gcccggcgcc gtcgaccttc acgaggagcc ccttggcgcc
     3241 cgtgccggcg aggagcgcgc cgtccttggc ccacgcgagc gcgagcacgt tcacgtcctt
     3301 ggtctcggca tagacggagc ccttacccgg gcccgtcacc tggaagatct tcccgcgcgg
     3361 gccggtgccg gcgtacagcg cgccgtcctt gccgaccagg agcgaccaga tcgactcctc
     3421 gcccgtcttg aagtagcgcg tggtgtcgcc gcgcgccgtg atccggaaga tctcgccacc
     3481 gggggccgac gcggcgtaca cgtcgtcctt ggcgtccacg gccatcgccg tgatctcgct
     3541 ggcgcccgtc ttccagagga gcttcggccc ctcgccgcgc gtccagcggt aaatgccgcc
     3601 gttgtcgccc gtgccgaaat agacccgtcc cttcgtgtcg cgcgccaggc tccagatgta
     3661 ggccacgtcg ggcaagtggg tcacgaccga atcccagccc ggtccgaggg tgagatagcc
     3721 gtcgctgtcg atcgcgacgc cgacgacctg ctcgccctgg aggaagtccc cggcgctgtc
     3781 cagccgccag aaggacgtgc tcacggcgtg cgcggagggt gcaagacacg ggagtgcgag
     3841 cgccgcggcg aacagcgcgg cggcgcgggc ggaagcgcgg agggtcgtca gaacgtctct
     3901 cctttcgcga cgggcctgct gggcgggacg tccggcgtca tcgcacgccg ggaatgacgg
     3961 tgacggtggc gctctcgcat ccgcgcacga cgtgtccgag cgggatcgcg agttcggaca
     4021 cggtcgcgcc cttggtggtg gccgactctc ccgacttggg cgaggacccg agcacgttga
     4081 gcacggagcc gggcgcctgg gacatctcgc gaccgccgac gacggcgccg ccgacggtgc
     4141 ggtagagctg cacgtagatc ttgtcggacg gacggtccga ctcgatcagg tcggcgagct
     4201 ggtcgaaagt ctcgggctgg taccgctccg gggcgcgctg catctcccac ttctcggtct
     4261 cggtggcgtc gcagacgcgc acggtgagat cgccggccgg cgtcgcgggc ggaacgcgga
     4321 cccgcacgcg gcgggtctcg accaggcggt tcgagcggcg gagcgtgagg tccacgtcga
     4381 gcgaatcgcc cgcgatgatc gcggcgggcg cgacgcggat cccgagcagc gccgcctcgt
     4441 cgatcccgtc ctccacgcgc acgtccacgc ggaccgagtc gaggcgcgag gggcggaagc
     4501 ggtcgccgag gagcagcgtg agcgactgtc ccacggtctc ccccaccccc gagacgggcg
     4561 cggtgctgat gaaggtgtct tccttgtgga tcgtgcgccc gccgccgttc atgtagagcg
     4621 cgaactcgta gcgcagcgtg gacacgccgg cgtcgtagag cgcttcggag atggagttca
     4681 cgaccgtcgc gctgacgagc gcgggggtga gatagcggct ccgcgccacc tccatgtggt
     4741 acgagcggga gcggccgccg cttccggcga tcgcgaccga taccggaatc atcgacgggg
     4801 ccgggcccat cgtccccgcg atcccgacgc tccggtcggc gaagatcgtg ccgcaggtca
     4861 cggtgggcga gccgatcttg ttcgagatct gctggctcga catgatcgta tggatcttgg
     4921 ccgccgtcat cggcagctcc atccagccca tcgcgaggaa gggatgcccg aaggcgagaa
     4981 cgcggtcgcc gtcgcgatac gtcaccgtgc cgatcgcggc cgccgaccag tcgccgcgca
     5041 cgagctggac gccgatggcc gaaccggggc ggagggagtc gcaggagccg cccggcgcgt
     5101 tggcgcctcc ggggctcgcg acgaagccgc gctcggagag ccagggctcg aggtagtgga
     5161 tcgcttcgtt ggtgaagccc gcaagcgcga gcggcgtggc gatcggccgg gcgccgccgg
     5221 cgtcggcgga ctgctccttg ggcgcgccga gggacccgaa ggcgtcctcg gcgttggggg
     5281 gcttggcgcc cggcgccgga agaaggctca gcatctgatc gatcggcgtg atcccggcca
     5341 tcggctcctt caggaaggac cacgtgtagg agacggcgcc gatcagcttg ccgccgatgt
     5401 acaccgggct gccgctcatc cccgcgatga caccggtcct gtccaccacc ggacccgacg
     5461 ccttcgccat gatcaggtcg aagccggggc gatagccctt cagcaccccg acgatcgtga
     5521 cggcaaaggt gtcgatctgg gtgccggaga agacgctgta gccggtgccg gtcatcccct
     5581 cgcggacctc ggagaccggg agcgtggccg aaggggggat gaggggcagc agggccgccc
     5641 cagccgcggc gccgggcagg gttcccccga ggaggacccc aaggattccg gaggcgagga
     5701 ggagagccgg taggacgcgc ccgagcgcgc gacgggtggt cattagtagt gggaacaata
     5761 cggactggag gaaggttccg tcaacgagtg ccgccgcttc cccgcgcccg gtcgcgcagc
     5821 gaggccaggc gctggagcgc gtccagcggc gtgagccgct ccacgggggc gtcggccagc
     5881 tcggcgagga gcgcggcccg ctcgtccgcc cccacgcccc gcgcggcacc gccggtccct
     5941 tccctccgcg cgccgttctg gccggccgcg tgctcccgct ccaggcgtgc caggatctcc
     6001 tcggcgcggg cgagcacctc gcccggcacg ccggcgagct gcgcgacgtg gatcccgtag
     6061 gagcggtcgg cccgccccgg cgcgatcacg tgcaggaaga cgatctttcc ctcgttctcc
     6121 gagacccgca cgttccggtt cttgagccgc gggagcgtcg cctcgagatc ggtcagctcg
     6181 tggtagtggg tggcgaagag ggtgcgcggg cgcgcgcgcg gtccctggtg caatgcctcg
     6241 gtcaccgccc acgcgagcga gagcccgtcg taggtgctcg tcccccgccc gacttcgtcc
     6301 aggagcacca ggctccggtc ggtggcctgg cgcaggatgg tcgcggtctc ctccatctcg
     6361 acgaagaagg tcgaggcgcc gcgcgcgatc tggtccgagg cgccgacgcg cgtgaagatc
     6421 cggtcggcca gcccgatctc ggccgccgcc gcggggacgt aggagccgac ctgcgccagg
     6481 tagaccgcca gacccacctg gcggaggaag gtggacttgc cccccatgtt ggggccggtg
     6541 atcagccaga tctgctcccg ctcgccgtcg agcgcgcagt cgttcgggac gaacgcgccg
     6601 ccgtccagcg cgcgctccac catcggatgc cgcgactgct tcagcacgag cttccgatcc
     6661 tcggtcaggg ccgggcgcgt ccatcgctcg cgcgcggcgg tctcgccgag cgcgcggtac
     6721 aggtccagct ccgccaccac ccgcgccgcg tcgtgcaggg cgccgatcga ctccaggcag
     6781 cgctcgcgca ggcggtggaa atgcgccgct tccgagcggc ggctctccac gctggccgct
     6841 tcgatccgct cctccatccg cttgagctcg ggggtgacgt agcgctcggc cccggccagg
     6901 gtctgtttcc gaatgtagtc ctcgggcacg cggttgagct gggagcgcgt cacctcgatg
     6961 tagtagccga agacccggtt gtagccgacc ttgaggttgg cgatccccga gcgcgcgcgc
     7021 tcccgcgcct cgagcgcgag cacctgctcg cgcccgtcgc ccgcgtcgct ccgcatggcg
     7081 tccaggtcgg ggtcgaagcc cgggcggatg attccgccct gggcggcggt gagcgggagc
     7141 tcctccatca gcgcttcttc gagcagggcc gcgagcccgg agaggtcggg aagcgactcg
     7201 cgccaggcgc cgatcgcggc tccggtccgc gcctcgagcg tgaggcgcac ggcgggcagg
     7261 cgccggagcg cgtcgcgcac ggagcccagg tcgcgcggcg ccgcgcgccc ggaacccagg
     7321 agcccgagcg tgcgctcgag atcgggaaag cgatggagcc gctcggccaa ttcggtccga
     7381 agcggcgccg actcggcgaa gcaggccgtg cggtcgaggc gcgccgcgac caccgccgtc
     7441 tcgcgcgccg gcttccggag gaggctccgg agggcccgag cccccatcga cgtctcggtg
     7501 cggtcgagca ggtggagcag cgaatgctcc tttcccgccg gcgattcgaa gacctcgaga
     7561 ttgcgaagcg tcacctcgtc gacgacgagc ggctccccct cgcgaagcgg gcggatctcc
     7621 cggatctgcg gcaaatccga cttcgcgagc gagcggaggt agtcgagcag cgcgcccgcg
     7681 gcgcccagcc cctcgcgtcc cgcgtccagc ccgaaggaat cgagtcgcgc caccgagaag
     7741 tgccggagca gcgcctggcg cccggcctcg ggatcgaacc gcgccgtctc gcgacgcgtg
     7801 atcggcgtct tgccgtaggg cccgctctcg ccctccccgc acaccggatc gcgcgcgccg
     7861 cggcccggga gcggcatcag cacttcgcgc gcggggaagg tgagcgggag tgccgcgagc
     7921 ccctcggcgt ccaccgtccc cgcgcggaac tcgcccgtcg agacctcggc cacggcgacg
     7981 ccgacgcgcg cctcgccggg ggcgagcgcc acgatgtagt ggctctcggt cgcttccaag
     8041 aggcccagcc gcgtcaccgt gcccggcgtg agcacctcga ccacctgccg gtcgaccagc
     8101 cccttcgcct cctcggcgct ctccatctgc tcggcgatcg cgaccgagta gccgcgctcg
     8161 accagcttct tgagataccc ctcgaccgag tggtggggca cgccggcgag cgggagcggg
     8221 tcgcgcttct tgtcgcgcga ggtcagcgcg atgccgagca cgcgcgaggc gatgagcgcg
     8281 tcctcgccga aggtctcgta gaagtcgccc atgcgaaaga ggaggatgct ccccgggtgc
     8341 tcggccttga gcctccggta ctgctcgagc atcggggtcg tctcgccgcg ggcggagacg
     8401 gcctccgccg cggcgttcgc ttccatgcgg gccggggtgg cttccacggg gccctagcgg
     8461 ggcacgacct ggtaggagag ccctcgctcg cgccggagcc gctcgcccag cgcgcgcgcc
     8521 tcatcgcgcg tcgcgaacga gcccgcgcgc acgcggaaga ggggcggcgt ctcgctcccc
     8581 tggcggaccg acacgtcgtc gttccccgcc gcgcgcagct cgcgcgcgag cgcgtcggcc
     8641 aggtcccgcc gcgtgaaggc gccgatctgg atcgcatagc cgccgccgat cggcagaagc
     8701 ggcgtcaccg tgtctccggg acggatgccg cggtcgcgaa cccgcgccgc ctcgtaggag
     8761 agcgggaagc gctgcgccac ctggcggaag acgctggccg cgtcggcctg gcgtcccacc
     8821 gcttcgaggg ccgtgcccat cgcgtagagc gcctgcggcg cgtagtcccc ctgcgggtgg
     8881 cgctccagga gcgtccggag cgcggccatg gcggccgagg gcttgctggc gcggagcgcg
     8941 gcctccgccg tggcgtactc gtaggattct tcttcgggcg agcccttgag gtcggattcg
     9001 gccgaggcca ggaactgcag cgcgcccgcg gcgtccccac ggccgagctg cgccagcccg
     9061 cgggtcacca agccctggcg cccggtcgcg ctccgctcca cgcccggggt gaagatggcg
     9121 agcagattct cggcctgcgc gtagtcgccg ctcacgtagc ggagccgggc cagcgccagc
     9181 gtcgcctcgc gccggaagac gcctttcgga tagtgcgcca gatagtccga gagcgcctcg
     9241 tcgtaggcgt tgccgtcctc gaggacgagg gcgcgcaggt acgccgcggc gtcggctttg
     9301 tcgggagtca ggccgttttc gggaatccgg atcaggatgg cgcgggcctc ctcggtgcgg
     9361 ccccggtcga aggcgtcgcg cgccgcgacg aggagggccg cgttcgcttc ggggccggag
     9421 ggcttcttcg cctcttttcg gggcgccgcc gcgacgagcg tgaccgcgcc gccgaggagc
     9481 aggacgagcg cggcggcgcg caggccgtgc ctcacgcgcg ctccagccag gcgaggcagg
     9541 agggacagcg cgaccagtat tcctcggcgc gctccccgca cttggggcac gcgggcggcg
     9601 ccgagccgcc cagctcgcgg agcagcgtgt ccacctcggt cagcgcccgc gcgggatcgc
     9661 ccaggcggcg gtggatctcg atcaccagga gccgcgcctc gagcgaatgg ggatccaccg
     9721 tcatcgcctc gcgcgcggca tgcgtcgctt cggagaggtc gccgcgcttc atgtgcatgc
     9781 gcgccagcgc gagcaggatc cgcgcgtcgc gcgggttctt ctgcagcatc tgctcgtaga
     9841 gcggtgccag gtcgccgaag cggccccgct cgaagtagga gacctcgagc cgctcgagca
     9901 ccaggtgcga gtgctcgggg tgcgcggccg cgaggccctc ccagagcgcc atggcccggt
     9961 ccggatggtt cgtctcgtag tagatgtccc ccatccggag cagcgccacc gggagatcgc
    10021 gctccagctt gagcgccgcc ttgaagtgct tcgccgcctc ttccagatgc tcgttccgga
    10081 ggtagacctc gccgattgcg gcccgatagc gcgcgagcgc cgcggtctcc ttctcgccgc
    10141 gcgccttggc catctcgacg cgcacgtcgt aggcgcggtc ccattcgccg cccgcctcgt
    10201 acgcctggag caggagcggg aggccgctgc cgttcttccg ctcgcgctcg cgcaattccg
    10261 aggcctccgc gatcgcctcc ttcggacgcc ccaccgcgat ccagtcgagc accagcgcct
    10321 cgcggatcgc cttgtcctgg gagggtgtgc gcgccgcgcg cgcggtgagt ttccggtgca
    10381 ggtcgagggc gcgggcggcc tcgccgcgct cgcgaaggag gttgccgaga tggatgtagg
    10441 cgtcgatgtt cccgctgtct tcctggacgg tatcggcgaa cgcctcgagc gcggcgtcct
    10501 tgtcccccgc gagcagcgcg tgcacgccgc gctggtacgg cgtctccgga cggggcggtc
    10561 cggtgttcgc gtcgcgggag aagagccacg ccgcgtaccc caccgccgcc agcgccccca
    10621 ccaccgccac ccaggtgaac agcggcgcca tcagcgccct ccgcgcccct cgggcatcag
    10681 cgtcccccgc cggccttgga ccccagcgcg agcccctcga gcggaagatt ccggagctcc
    10741 gccagctccc gctccagctc ctgcttctgc cgctccgccg cggccacttc cgagcgggcc
    10801 ttcagcacct ggaagagcac cagcaaaaac gcgaggaaca ttcccgcggc gaacgcggcg
    10861 aataccgcga cgttcacggg aatggagcgg tagacgtgcc cgggcaggtt gatcgccgtc
    10921 acgctctcgc gcatgttcag gatcgcgaag cccacgacga gaatcatgac ggcgagccaa
    10981 aggacgttcc ggagaaacca catggcacgg cctccttggg gggagatgga cgcgggcgct
    11041 gccgcgccga tccgcgccag aatagaggtg taccgcgccc gggtcaaccg atggcggcgc
    11101 ccgccagggc gccattgcga agcgcctcga tgcgcacggg gagaatgccg cccgccgcgg
    11161 gacggcgttc gagccggacg ggcgcataga tgtcggtcat ggcgatgcat gccggttccg
    11221 agagctggcc gcgttccgcg atggctggac gaacggagcc gatcagactc cgatagaagg
    11281 cgtcgcgctt ggcgtcgccg agggcccgga gccgctccga ccgctcgcgc gcctccgcga
    11341 gggacgccgc gtcgctctgg accgacgccc gcgtgccggg acgcggcgag tagcggaaga
    11401 cgtgaaggtg cgtgatcgga aggagcgaca cgaagcggca ggtctcctcg aactcggcct
    11461 ccccttcccc cggatggccc acgatcacgt cggcgccgat ggccgcgggt ccgccgccgg
    11521 cgagccgctc caccagccgt gcgtagtcct cggggcggta ggggcggcgc atcgcgcgga
    11581 gcaccgcggg ggatccgctc tggagcggca ggtggaaatg gcggcagagg cgcggctccg
    11641 atcccgccag ggcggccagc gacgggtcca ccttgttcgg ctcgatggag gaaatccgca
    11701 cccggtgcga ggggccgagg gagaggatcg actcgaccag ggcggcgaga agcccgcggc
    11761 ggccgagatc gcggccgtag gcgcccaggt cggcccccgt caggacgatc tcatgaaatc
    11821 cggcatcgag aagacgccgc gcccgggcgg tcgcctcggc gaggggcaga ctccggctcc
    11881 tcccgcgcac gtaggggacg acgcagtagg agcagaaggc gtcgcagccg tcctgcacct
    11941 tgagcagggc gcgcgtgcgt ccgaaagcga gcggaatggg tagggagtct ccggcagggg
    12001 cggggggccg cttcgcgcgg gccaaagcca cggcgatgac ggcaaggtcg ttccgctgca
    12061 tgatcggatc gcgcggcacg accgcgcccg ccgcgcccag ccgctccgcg atcgtctcgc
    12121 gctccgcgat gcccatgaca tccgacacgc ccggaatcgc cgccacttcg cgcggcgcgc
    12181 gctgcgcgta gcaccccgtc acgacgaccc gtgcgcccgg gtgctcgcgc gcgagccgcc
    12241 ggatcgcctg gcgcgcctcc tggtcggcgc gcgcggtcac ggtgcaggtg ttcaccacca
    12301 ccacgtccgc cgcctccccc tcgcgcgcct ccgcccatcc ggcgcctccc agcgccgcgc
    12361 gcatggcggc ggtgtcgtgc tggttcaggc ggcagcccag ggtgaaatag aaaacccggg
    12421 gcgccgtcgc ggacgccccg ggtcggattt cgtacggcat gaacgctacg cggccgtcgt
    12481 gtcgtcgctt ccttcagcga ccggcgccgg ctcgcccgtc tcctcggcgg ccgagggcga
    12541 gggcttgaac cgctcctgaa acgcctcctg cccggcccgg tcctgatcgg cgacccaggc
    12601 cttggcgctc aggatgatgc ggtgcgcctg cgtgtcgacg cgcgtgacct tgaggtccag
    12661 cgtgtcgccc ggcttgaagc tgtccgacgg cttcttgagc ccatccacgc ccagctgcga
    12721 gagcgggatg aatccttcga ggccgtcttc caggtccacg atgacgccgc ggtccagcag
    12781 ccggaccacc gtccccttca cgatgatgtc ggggaggtag cgctcggcca gcgtgggcca
    12841 cggatcgtcg ctcacctgct tcagcccgag gctgatccgg cgcgcgtcct tgtcgatgga
    12901 gagcaccacc acgtccacct tgtcgccctt cttcaagatc tcgctcgggt gggcgacgcg
    12961 gcgggtccac gacatgtcgc tgatgtggac cagtccgtca atcccctcct cgatctcgac
    13021 gaaggctccg aagttggtca ggttgcggac cttccccgag agcttggttc cgatcgggaa
    13081 gcgatcgtcc agcgtgagcc acggatcggg ctcgatctgc ttgagaccga gcgagatctt
    13141 ctcgttgtcc ttgtcgatct tcaggatcat cgcctcgact tcgtccccga tcgcgacgac
    13201 cttgctcggg tgacgcacgt ggcgcgtcca ggacatctcg gagatgtgga tgagcccctc
    13261 gatccccttc tccagctcca cgaaggcgcc gtaatcggtg atcgagacca ctttcccctt
    13321 cacgcgctgg gtcacgttgt agcgcttgtc cacgtcctcc cacggatacg ccgtgagctg
    13381 cttcatgccg agggagatgc gctccttctc gggatcgaag gagaggacct tgaccttcac
    13441 cttgtccccg atcgagacca gctcggaggg atggctgacg cggccccagg acatgtccgt
    13501 gatgtggagc aggccgtcga tgccgcccag gtcgacgaag gcgccgaagt cggtgatgtt
    13561 cttgaccacg ccctcgcgaa tctggtcctt ggccagctcc tggatgatct cgctcttctg
    13621 cttcgcccgg tgctcctcga gcaccgcacg ccgcgagacc acgatgttcc ggcgccgctt
    13681 gttcagcttg atgatcttga attccatcga ctggccgagc agcgaatcga cgttctgcac
    13741 ctggcgcagc gcgatctgcg aacccgggag gaaggcctcg accccgtaga ggtcgacgac
    13801 cacgccgccc ttgatcttcc gcatgagccg tccctcgacc agctcgccct ggtcggcggc
    13861 cgccttgacg cggtcccaga ccttgacgaa atcggcgcgc tgcttggaaa gaacgaccag
    13921 gccgtcctga ttctcggtct tctcgaggaa gacctcgatc tcgtcgccga ccttcacgcc
    13981 ttcgaggtcg gggaactcct cgaccgggat gaccccctcg gacttgaagc ccacgtcgac
    14041 ggtgacttcc ttgtcgtcga tcttgagcac ccgcccgcgg acgatctccc cctcttcgag
    14101 gtcctgaagc gactcctcgt agagctggag ccattgcccc atggcgcccg cttcgtcctc
    14161 gccgtcgacc tcgacggccc ccacggcggc gcccggcgag gtgaacagct cctcgtcgtc
    14221 gtcctggtgg atcagcctcg tggtcgtgcg ccgcttccgc ggcttggctt cggcctccgc
    14281 ctcatccgcg ccatcgtcct tcacttccag ctcttcttcg tgacgtcgaa ccattccgtt
    14341 acgttacccc ctgtttctat gaatttcggc ctcgagggcc ggtgctgccg ttcgtcgttg
    14401 ctacgcggag cgggcctgac ccggctccag atcggtgaga cgcgccacca cttcggcgat
    14461 cacttcgtcg ggcgtggacg cccccgccga gacgccgacc cggcggacgc cggtgaacca
    14521 ggacgggtcg atctcttcgg ccgactcgac ctggtgcacc cgcggattca ccttgcggca
    14581 gacctcggcc agctgcgccg tgttcgcgct ctgccgaccg ccgatgatca ccatcgcgtc
    14641 caccgagggc gcgagctcga cggcggtctt ctgcatcgac gtcgtcgcgc cgcacagggt
    14701 gttgaacacc cgcacttcct tcccgcgctt caacagcgcg ccgaccacct cctgcacgtg
    14761 gccgatcgag tgggtcgtct ggaacacgac ccccatgcgc gggatgaact tcagcttctc
    14821 cgcctcttcg acggtgtcga tgacgatcgc cttgccctgc gtgtgcgcca ccacgccgat
    14881 cacttccggg tgatgcgcgt cgccgatcat gacgaccttg tagttctcgg cgatcagctt
    14941 cgccgcgtac tgctgcgcct tggtcacgaa gggacaggtc gtgtccacga ccttgagacc
    15001 ccgcgacgtg gcctgctcgt gcacctcggg tccgacgccg tgggagcgga tcaccacggt
    15061 gccgtccgga gccagctcgg tcagatcgct caccacttcc agtccgcgcc ggtggaagag
    15121 actcaccacc tgcggattgt gaatgagctg accgagtgac ttcacgggac cctgcgcctc
    15181 gtccagggtg tcggtcgcca tcttgatggc gcgcttcacc ccgaagcaga acccggcgtt
    15241 gtcggcgacg acgacttcca tgcaatcccc cgccgggctc agcccggcag cgcggcgatc
    15301 gcttccatca cggccgccgc gaacgagttc atggcctccc ggtccgcatc gacccggatc
    15361 ggcggcgtca tcggcgtgcc gacgacgatc cgaaccagcg tctgacgacg cagggagcgc
    15421 cgaatctggt tggaccccga aatccggatg gggagcaccg gcgaccgggt cacgaccgcg
    15481 aggcggccga taccggcccg aggcgccaga agggtaccgc tcttgttgcg ggtcccctcc
    15541 ggaaatatga gaacggctcc tcccccgttc agcacttctt ccgcgccgag cagacccgac
    15601 ctggcccggg tgcctcgctg aatggggatc gaattatacc cccggagcat cgccccaaac
    15661 accttatttt ggaagagttc cgccttcgcc acgaagtgca ggacccgggg gaaattggac
    15721 cccaccaggg gcggatccca gaagctgatg tggttgcagg cggcgatcaa gggcccgctc
    15781 cgcggcacat gatcgagccc ctcgatgcgg aacttgagga aagtccgcga aaatcgcttc
    15841 acaaagcggc ttccgaacca gtagaagggc ttggtcgggc cgggtccccg gcgggccagc
    15901 tcggcccagc gggtcaccac cgcgacctgc tcctccagcc ccaggtccga ggtgtcgagc
    15961 cggatggcgt cccccgccgg caggagcggg ctctccgccc tcccctggtc gcgggcgtcc
    16021 cgctcccgga tcatcgcctc gatcgcggtc aaatcggggc gctcgccccg gctctccagc
    16081 tcccgaaagc ggcgcagggc ccgcaccgcc accgaagcct cgaggaagat cttgaggtcg
    16141 gcgcgcggga agacgaccgt gccgatgtcg cgcccctcca tgaccacgcc cggggggctc
    16201 gcgaacgagc gctggatggc caccagccgg cggcggagac ccggatggac cgcgacgcgc
    16261 gaggcggcgt ctccggcttc cgcggtgcgg atctcgccgt cgagcagccg tccgccgcag
    16321 cggagaccct cgggcgtcgg ctcgaagcgg accccctcca gcgagcggag catctccgct
    16381 tcggagtcga ggtcagcacc cgcctgcagc gcgacccacg ccgccgcgcg gtacatggcc
    16441 cccgagtcca cgtgcgggag ggccagggcc cgcgccacgg cgcgcgcggt cgcgctcttc
    16501 ccggcgccgg cgggaccgtc gatggcgacc acgaaagccc tcaacggcga aggctccgga
    16561 gcctgtcgag aaaatcggga tcggagatcg cggccgcatc ggcgccttcc accgtgacgc
    16621 cttctttcgc gacgaggccg gccacgagga aggccatggc gacgcgatgg tccccggccg
    16681 cgtccacgcg gccgccgcgc agcgagccct tcccctcgat gatccagccg tcttccagct
    16741 cctccacggc gccaccggcc gcgcggagcg ccgccgccac ggtcgcgatc cggtccgact
    16801 ccttcacgcg cagctcgcgc gcgccgcgga tctcgctccg tcctttcgcg aacgcggcga
    16861 gcaccgcgac catcggaagc tcgtcgatca ggcgcggcgc ttcgtcgtcc gcgaccgtta
    16921 cggggcggag accgcccgct ttcacgtgaa ggtcggccag cggttcctcg cccgctcccc
    16981 tcgcttcgag ccgctcgatc cccgcgccca tccgcatgag gagatcgagg aacgccgtgc
    17041 gggtggggtt gacgccgacg ccgcggatcg tgatctcgga gcggggcacg agcgcggccg
    17101 cggccaccag gaaagcggcc gccgagaggt cccccggcac ctcgatctc
//
LOCUS       DASSLZ010000064         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1038935, whole genome shotgun sequence.
ACCESSION   DASSLZ010000064 DASSLZ010000000
VERSION     DASSLZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038935"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2399)
                     /locus_tag="VFM00_02185"
     CDS             complement(<1..2399)
                     /locus_tag="VFM00_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7497085.1"
                     /translation="MLLVFQPDARPERVRAILKDLGATKIHTSRRSHVERHRITKRVE
                     DAVAQYRNDPAVRIIEPNYLLHADAVPDDPSFAIQWALQNTGQQGGTPGADIHAVAAW
                     DQTTGSNQVVVAIVDTGMDLTHPDLVSNLYTNPAEISGNGLDDDGNGLVDDVHGFDFA
                     NFDADPSDDNGHGTHVAGTIGAHGDNGIGVAGVVWHVRLLPLKFLDAGGTGTTADAVD
                     CVDYAVLAGASVINASWGSPERAQSLELAIQGANEEGVLFVAAAGNTGSSNDTFGNYP
                     ASFPEPNVIAVGASSRTDDRASFSNFGVNGVDLFAPGVQILSTLPRGLYGLNSGTSMA
                     APHVSGALALLKSRFPGMGAATMKEVLLSATDRIPALSGLCVSGGRLNAARMLEGVDS
                     TSPGPIADLASSFVSSDRVTLAWTATGDDGGSGTATEYEIRYSLAPLDASTFASATRV
                     AGTPVPRAAGSREEASIGGLAPLTDYYVAMVARDEFGNSSPLSNVITFRTTAPPAAAV
                     DPTSLDASLLTGQRQAFTVDVRNDGAGALDFTVQALGAGGVPAPRWLSVLPGGGSAVG
                     GGRFPIAVTMNADALPGGDYAGLVRLTTNDPARRTLDVPVGMHVTAAPDLDVDPTMIA
                     FGPIPVGTSDTEELFITNSGFADLHVDSVRVEGSDFSADPGGFDLPPGQSWDLFVTSA
                     PTHTGTVTGAVVIASNDPDQPVTRVPLAAGGIIPPVAAFAPVHQDAVLHTGQTTTRTF
                     TISNSGGSALSWSLRPRSAASLEFDSTGSLTAALAPEPVSVPSTSGPAELGASGASGV
                     VA"
     gene            2692..>3483
                     /gene="thyX"
                     /locus_tag="VFM00_02190"
     CDS             2692..>3483
                     /gene="thyX"
                     /locus_tag="VFM00_02190"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET7497086.1"
                     /translation="MALADSPSRSQAPARGVAPELDRVLGTRFRVLDDGFVRVVDYMG
                     SDESIVQAARVSYGEGTKKVHEDRGLIRYLMRHQHTTPFEMCEIKLHVRVPMDCWRQW
                     IRHRTANVNEYSTRYSVAIDSAQTTAPEEWRAQSSENRQGSDQFLDPEQGKALSAEER
                     ALQEKAREVYEHRLAEGVAREQARKDLPLSTYTEAYWKVDLHNLLHFLALRMDRHAQA
                     EIRAYSETIGREIVKRWCPIAWEAFEDYRLHSLVLSRIEREVVAAL"
BASE COUNT          542 a         1173 c         1272 g          496 t
ORIGIN      
        1 gcgacgactc ccgacgcgcc cgacgcgccg agctcggcgg ggccggaagt cgacggtacg
       61 gatacgggct ccggagcgag ggccgccgtg agcgagccgg tcgaatcgaa ctccagggac
      121 gcggccgacc gcggccggag cgaccaggaa agcgcgctcc cgcccgagtt cgagatcgtg
      181 aaggtgcgcg tcgtggtctg gcccgtgtgg agcacggcat cctggtgcac cggcgcgaac
      241 gccgcgaccg gcgggatgat tccccccgcg gcgagcggca cgcgcgtcac cggctggtcg
      301 gggtcgttgc tcgcgatgac cacggccccg gtgaccgtgc cggtgtgggt cggcgcggag
      361 gtcacgaaga gatcccacga ctgccccggc ggaaggtcga acccgccggg gtccgccgag
      421 aaatcggaac cctcgacgcg gaccgaatcc acgtgcaggt cggcgaagcc cgagttggtg
      481 atgaagagct cctcggtatc cgaggtgccg acggggatcg ggccgaaggc gatcatcgtg
      541 ggatccacgt cgagatccgg tgcggcggtg acgtgcatcc cgaccggaac gtccagggtc
      601 cggcgcgcgg gatcgttcgt ggtcagccgc acgagcccgg cgtagtcgcc tccgggcagc
      661 gcgtcggcat tcatcgtcac ggcgatcggg aatcgccctc cgccgaccgc gctcccgccg
      721 ccgggcagca cggagagcca gcgcggcgcg ggcactcccc cggcgcccag ggcctgcacg
      781 gtgaagtcga gcgcgcccgc gccgtcgttg cgcacgtcca cggtgaatgc ctggcgctgg
      841 ccggtcagca gcgacgcgtc cagggacgtt ggatccaccg cggccgccgg gggggccgtc
      901 gtgcggaacg tgatcacgtt ggagagcggc gatgaattcc cgaactcgtc gcgcgccacc
      961 atcgccacgt agtagtcggt gagcggtgct aagccgccga tcgaagcctc ttcgcggctc
     1021 cccgcggccc gcggcaccgg cgtcccggcg acccgcgtcg ccgaagcgaa ggtggaagcg
     1081 tcgagaggtg ccagggagta gcggatctca tactcggtcg ccgtgccgct ccctccgtcg
     1141 tcaccggtcg cggtccaggc cagggtcacg cggtcggagg agacgaagct cgaggccaga
     1201 tcggcgatcg ggcccgggga cgtcgagtcc acgccctcca gcatgcgggc cgcgttcagc
     1261 cgccctccgc tcacgcagag ccctgagagc gccgggatcc gatcggtggc cgagagcagc
     1321 acttccttca tggtcgccgc ccccatcccg ggaaagcgcg acttgaggag cgcgagcgcg
     1381 ccggacacgt gcggcgccgc catcgaggtt ccgctgttca ggccgtacag cccgcgggga
     1441 agggtgctca ggatctggac gccgggcgcg aacaggtcga cgccgttcac cccgaagttc
     1501 gagaaggagg cgcgatcgtc cgtgcgggag gaggcgccca ccgcgatcac gttcggctcg
     1561 ggaaagctcg cgggatagtt cccgaacgtg tcgttgctcg agcccgtgtt ccccgcggcg
     1621 gccacgaaga gcaccccttc ctcgttcgcc ccctgaatgg ccagctccag cgactgggcg
     1681 cgttcgggac tgccccagga ggcgttgatc accgaggcgc ccgcgagaac cgcgtagtcc
     1741 acgcagtcca ccgcgtcggc ggtcgtgccg gtccctccgg cgtcgaggaa cttgagcggc
     1801 aggagccgca cgtgccagac gacgcccgcg acgccgattc cattgtcgcc gtgcgccccg
     1861 atcgtccccg cgacgtgcgt cccgtggccg ttgtcgtcgc tgggatcggc atcgaagttg
     1921 gcgaagtcga aaccgtgcac gtcgtccacc agcccgtttc cgtcatcgtc cagcccgttg
     1981 cccgagatct cggcgggatt ggtatagaga ttggagacca ggtcgggatg ggtcaggtcc
     2041 atcccggtgt ccacgatcgc caccacgacc tggttcgatc ccgtggtctg gtcccacgcg
     2101 gcgacggcgt ggatgtccgc gcccggcgtg cccccctgct gtcccgtgtt ctggagcgcc
     2161 cactggatcg cgaaggaggg atcgtcgggg acggcgtcgg cgtggaggag atagttgggt
     2221 tcgatgatcc ggaccgccgg atcgttccgg tactgcgcca cggcgtcctc gacccgtttc
     2281 gtgatccggt ggcgctcgac gtggctccgg cgcgaggtgt ggatcttcgt ggcgccgagg
     2341 tccttgagaa tggcgcggac ccgctcggga cgcgcgtccg gctggaagac gagaagcacc
     2401 tcgccctcga cgtagcgcgg acctttgggc ggagcggacg gtgcggtcga ggcggcgggc
     2461 gtgggcgcgg gagccggcgc ggagcgggcg tacgaaagcg gcgcggcgcc gaggaggaca
     2521 ctgaggagcg cggcaaacga tgtgcggaat cgggaaccag gcgcggcgct gcgacgcaaa
     2581 gatgaccccc aggacctctc cgagcccgtc tcacgataca ggagcgcgcg accttaggcc
     2641 agcccttccg ccgctgtgga cagggacttc ggccgggtgg agaatgaggc catggctctt
     2701 gccgacagtc cctcgcggtc tcaggcgccg gcccgcggcg tcgcccccga gctcgaccgc
     2761 gtcctcggga cgcgcttccg ggttctcgac gacggcttcg tgcgcgtcgt ggactacatg
     2821 ggctccgacg agtcgatcgt ccaggccgcg cgcgtctcct atggcgaggg gacgaagaag
     2881 gtgcacgaag atcgcggcct gatccgctac ctgatgcgcc accagcacac gacgccgttc
     2941 gagatgtgcg agatcaagct gcatgtgcgg gtcccgatgg actgctggcg gcagtggatc
     3001 cggcatcgca cggccaacgt caacgagtac tcgacgcgct actcggtggc gatcgattcg
     3061 gcgcagacca cggcgccgga ggagtggcgg gcgcagtctt cggagaaccg ccaggggagc
     3121 gaccagttcc tcgatccgga gcaagggaaa gcactctcgg ccgaggagcg cgcgctgcaa
     3181 gagaaggcac gagaggtcta cgagcaccgg ctggcggagg gggtcgcgcg ggagcaggcg
     3241 cgcaaggacc tgccgctctc cacctacacc gaggcgtact ggaaggtcga cctccacaac
     3301 ctgctccact tcctggcgct ccgcatggac cgccacgcgc aggccgagat ccgcgcctac
     3361 tccgagacga tcgggcgtga gatcgtgaag cgctggtgtc ccatcgcgtg ggaagcgttc
     3421 gaggactacc ggctccattc gctggtcctc tcgcggatcg agcgcgaggt ggtggccgcg
     3481 ctc
//
LOCUS       DASSLZ010000065        26860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_910637, whole genome shotgun sequence.
ACCESSION   DASSLZ010000065 DASSLZ010000000
VERSION     DASSLZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 26860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26860
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910637"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VFM00_02195"
     CDS             complement(<1..140)
                     /locus_tag="VFM00_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011477723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7497087.1"
                     /translation="MAKQKYERTKPHVNVGTIGHVDHGKTTLTAAITMVLSQKNPKIA
                     VR"
     gene            complement(267..342)
                     /locus_tag="VFM00_02200"
     tRNA            complement(267..342)
                     /locus_tag="VFM00_02200"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(307..309),aa:Thr,seq:ggt)
     gene            complement(778..853)
                     /locus_tag="VFM00_02205"
     tRNA            complement(778..853)
                     /locus_tag="VFM00_02205"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(818..820),aa:Gly,seq:tcc)
     gene            complement(913..994)
                     /locus_tag="VFM00_02210"
     tRNA            complement(913..994)
                     /locus_tag="VFM00_02210"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(958..960),aa:Tyr,seq:gta)
     gene            complement(1017..1091)
                     /locus_tag="VFM00_02215"
     tRNA            complement(1017..1091)
                     /locus_tag="VFM00_02215"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1057..1059),aa:Thr,seq:tgt)
     gene            complement(1201..3276)
                     /locus_tag="VFM00_02220"
     CDS             complement(1201..3276)
                     /locus_tag="VFM00_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7497088.1"
                     /translation="MLFLAVGSGFLVLATILLYQNGQAALRRQLASTATTAAETAAAL
                     IHADDHRLIRTPADMNTQAFRTIVDDLSALRRANPSIYHLFTLAPVGRLGAWGVVLDM
                     GGAAPVREETSLRGGRLPIGSPPPETVPADLIHAAMTSTACAILDLTKPDMARVVAVA
                     PIRTDSGESVGLVVVELSAAGLIAEAHLLWYVTLGIFLVGLGASVAASSAVSRWVTRP
                     VDELLRGVGEIARGRLETRIQVGARNELGQLGDAINLMAQSLEASQSRNAAQQARLRD
                     LHRLGSQTAATLELPRMLAVAARGLRAICGGDVVVAGAAGRRDRAIRVWAVEGRAVSA
                     DEWEVALEPVEKVLGAETRLLTRAELAAAGFTCLASIPGGYALTAPLRVNDETVGALV
                     AFGEAEQFHSDAVSLASLFAAQVAAAVGNARLFDQVRELDRSKSEFLSIASHEVRTPL
                     TVMKSSLDLLVSSSKFDETSDQRQLIAFCQESVDRLIRLVKDILDVSKIEAGVLSVQF
                     LPTSLNELIEKCLFWVPQLPGGQGIEVDARLPKEPAMVFADPSRIQQVLENLISNALK
                     FSKPGGRVSIELRAHEHEFEVIVSDQGKGIAPEDLERIFGKFTQVEDSATREQGGTGL
                     GLAICRGIIEAHRGRLWAESVPGQGSRFHFTLTRVVDTPIGDRADGEIPVGSLLAALR
                     SESTTARRR"
     gene            complement(3347..4789)
                     /gene="cysS"
                     /locus_tag="VFM00_02225"
     CDS             complement(3347..4789)
                     /gene="cysS"
                     /locus_tag="VFM00_02225"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET7497089.1"
                     /translation="MSGTPGAGIRVYDTRRRSKVPYDPVLPGRAGMYVCGMTVQDKPH
                     VGHMRYAVSTDAIRRYLESKGFVVTYVTNFTDIDDRIIARANEEGIPHGEVAERNIQA
                     FLRYGELLNLKPATHYPRATRHIPEITALIATLIEKGHAYASNGDVYFDVRRWPRYGE
                     LSGRNVDELRSGVRIEVGEAKRDPLDFTLWKGAKPGEPAWDSPWGPGRPGWHIECSAM
                     AMKYLGEEFDFHGGGQDLVFPHHENEIAQSEAATGRTFARYWIENGLVNLEGAKMSKS
                     TKHFFSIEDVVARVEPEVVRFYLQSTHYRSPIEWNEERLEEAGIAYARLRDALARAES
                     AGASAAGQGQDLDATRLLQEISELRRQFGEAMDDDFNTARAQGHLFEIAKAINRVADQ
                     ADAHPQERAALPVAGRHLRELGETIGLFWGGPPAAQALPLEVQELVRQRDEARMQMQW
                     GRADQLRDQIAALGYDLKDSKEGTKVRRRT"
     gene            complement(4815..5732)
                     /locus_tag="VFM00_02230"
     CDS             complement(4815..5732)
                     /locus_tag="VFM00_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET7497090.1"
                     /translation="MRPTPFRADAAEAFNYATLAIKVGLIALGALILIRVAGILVQRL
                     ERAIRAAGRGDEISREKRARTLGGVVRGLARAAIIVVALLMAVRELGLDITPAIATAG
                     GFGVAAGLGAQSLVRDWISGFFIIYDNQYVVGDVVQVAGVAGTVEALTLHHTEVRDGE
                     GALHFVPNGEIKVVTNLTKAWSSPIVRIPVSLTEDPARVASVLERYLAEAALEPALKP
                     LLRDAPRVLGMEDVSVGQYTMMLQARTLPEHRLAVARALRFGAVTRLRAEGITIHSTA
                     AESPAPGTVPGSAAKLRDARLHDAAADGA"
     gene            complement(5799..6884)
                     /locus_tag="VFM00_02235"
     CDS             complement(5799..6884)
                     /locus_tag="VFM00_02235"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7497091.1"
                     /translation="MKVVVLLGGNTPEREVSLRSGAGIGRALLDLGHEVTLLDTGTGR
                     ILPDPDAAQALGSGRAAPPAEAPAESLDDSGRLPVPGAGAGNLEIYAKAIPRAADLVF
                     IALHGGDGENGVLQAVLDLARIPYTGSGVLASAVSMDKSLSKRIFRDFGIPTPDWIEL
                     EAPADPAAAWAPAIGEAAIARLGGFPLIVKPNDQGSSVGISVAKRREDLLPAIEEARR
                     YSRLILVESFIEGREVTVAVLDERAFPVVEITPEGGWYDYQHKYTAGASRYEVPAKLP
                     PDLTERLLALARESYRALRCSGIARVDFRLAEDGTPWCLEVNTVPGMTETSLVPKAAG
                     AAGLSYRDLVRAIVESAPARTAARSGA"
     gene            complement(6910..7401)
                     /gene="ispF"
                     /locus_tag="VFM00_02240"
     CDS             complement(6910..7401)
                     /gene="ispF"
                     /locus_tag="VFM00_02240"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET7497092.1"
                     /translation="MDRELRVGNGYDVHRLERGVPLVLGGVALAFEKGLRAHSDGDAL
                     AHAIGDAILGALGLGDLGSHFPDTDSRWRGASSLDLLERIRELAASRGGRVVNVDATV
                     IAEAPKIAPHVAAMRDNLARALAVGADRVSVKATTNEGLGPIGRGEGIAAMAVALVEV
                     DRP"
     gene            complement(7403..8101)
                     /gene="ispD"
                     /locus_tag="VFM00_02245"
     CDS             complement(7403..8101)
                     /gene="ispD"
                     /locus_tag="VFM00_02245"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET7497093.1"
                     /translation="MELAVILAGAGSGSRMEGRGPKLLIDLAGKTLLERAAAPFLAHA
                     AVTEIVAVVPEALLGPARDALAQVPRERVVGARALAGGATRQESVRLGLESLDPRASY
                     VAVHDVARPLATAALIERVFAAAREHGAAIPALPIRDTVKEVEDGRVTRSVPREKLVG
                     AQTPQIFSRAILARAHAQERTTLDATDDAALVEALGLPVHVVAGEPSNLKITEPTDLI
                     VALALLRLRSSGEV"
     gene            complement(8109..9530)
                     /gene="radA"
                     /locus_tag="VFM00_02250"
     CDS             complement(8109..9530)
                     /gene="radA"
                     /locus_tag="VFM00_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET7497094.1"
                     /translation="MARKETSRARAAAGPFFCAECGNESPKWFGKCAHCGAWNSATEA
                     PAGGAARRATGAGRRAAGYAGVAPSAPVRLEAIETGAEDRWRTGIAELDRVLGGGLVP
                     GSLVLIGGDPGIGKSTLALEMAAALGAAGRSVLYVSGEESPRQTRMRAERTALLGKSE
                     NLWIHAEVDLEAIEAEVARLNPALLVVDSVQTLSLATLDAAPGSVSQVRECGLRLLRL
                     AKERGLPVLLIGHVTKDGTVAGPRTLEHMVDAVLYLEGDAHHGYRILSAAKNRFGSTH
                     EIGVFEMRGDGLIEVTNPSERLLRERGEPVPGSAVVASVEGTRPLLVEVQALVAPTHF
                     ANPQRVAQGVDVKRLAVVIAVLEKRARLSLAGADVFVNVAGGIRLDEPAADLGLALAL
                     ASSFRDRPLPPDRVMIGEVGLGGELRPVPQLSRRLAEAARLGFRSAIVPKRSLDAADA
                     PPSGIALLPSATLAEAIERTLAT"
     gene            complement(9557..10453)
                     /locus_tag="VFM00_02255"
     CDS             complement(9557..10453)
                     /locus_tag="VFM00_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET7497095.1"
                     /translation="MNPSRRNAVQVGIMGLAALALLLAGVIWIKEYRLGKKKMTYTAR
                     FQEVGNLAEGDPVNVRGVRKGAVTDVKLEDQAVRVELELDRDVILHPDASLRIANIGF
                     MGEKFLALEPGTAPGRYDHTKPIPGRFQSGVPEVISGAGDLLIEATELSSRLNVMLDA
                     LDPAVVERTSKNMERASGSLNSALGDNRQDLRQAILDFKAAAHDLRAIASQNKDQVNT
                     SLQNFDTASRKLSGLADQLSATAASLQRVAAKVESNEGSVGRAIADTTLYTEMRETIR
                     NTNELVKDIRKNPKRYLKIGLF"
     gene            complement(10464..11237)
                     /locus_tag="VFM00_02260"
     CDS             complement(10464..11237)
                     /locus_tag="VFM00_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7497096.1"
                     /translation="MGGGPRSDAAIEFQDVWKRLGRKDVLRGLNLEVRRGECMVIIGR
                     SGTGKSVLLKHVVGLLAPDRGHVCVNGMEVEHLPEKELLELRRGMGMLFQGGALFDSM
                     TIGENVGLPLREHTPMPETQIEIVVSEKLALVGLEGSERMRPSSLSGGMKKRAALARS
                     LVLNPKIMLYDEPTTGLDPITSDLINQLIRRLQQRLGMTSIAVTHDMRSAYHIADRIA
                     MLHEGRIHAMGTPDEIQATTDPALRQFIEGSSEGPLLPT"
     gene            complement(11257..12057)
                     /locus_tag="VFM00_02265"
     CDS             complement(11257..12057)
                     /locus_tag="VFM00_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7497097.1"
                     /translation="MTEPAEPPQNTWPERVAMGWLGDVGEVFILVGKVHRQIPAALAR
                     PGLIIDQMAHIGVGSMPLTVIVALFTGAVTAVQALYQMRDYVPMLYLGTVIGKSVVIE
                     LGPVLTALVVGGRVGASIAAELGTMRVTEQVDAMETMGISPTRFLVVPRFLAAVLMLP
                     ALTIFADIVAIFGGWLVAVLTMGVSSHTFVSGLRLFFQIQDVFSGLIKSFAFGGIIAM
                     MGCYFGLKSEGGAEGVGVATTHAVVASCLLILIVDYLLASFLFRVIFA"
     gene            complement(12100..13467)
                     /gene="dnaB"
                     /locus_tag="VFM00_02270"
     CDS             complement(12100..13467)
                     /gene="dnaB"
                     /locus_tag="VFM00_02270"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET7497098.1"
                     /translation="MEGPRVPPQAEEAEKAILGSMLLSRDAVGHAVEIVRSKDAFYVP
                     RHREIWTAMMALYEKNAAIDLVTVSEELRKRNQLEACGGYSYLTSLDQNVASPGHVEH
                     YCKIVLDKWTLRRLLEAGHTISDEAFAEAEEVENLLDRSEQRIFEIANDQLRQGFVPM
                     NRLVLAAFEAIDEYRKRKVHVTGIPSGYADLDEMTAGFQRADLVVIAGRPSMGKTSFA
                     LNIAENVAVGTRGKPGPGVAIFSLEMSKESLVQRLLCSMARVDIHKIRRGYASYDEYK
                     RLQNAAAQLHQAPIYIDDSPSLGILEMKAKARRLAAAGDLGIIFVDYMQMVRGPSRAE
                     NRQQEMSAISRSLKIMAKELNVPVVALSQLSRAVEMRGGDKRPMLSDLRESGAIEQDA
                     DVVLFVYRHDIYETEPDKKDGKAEIIIAKQRNGPTGSVDLAFLGECTRFDSLRNVPEP
                     RGPHV"
     gene            complement(13514..14290)
                     /locus_tag="VFM00_02275"
     CDS             complement(13514..14290)
                     /locus_tag="VFM00_02275"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET7497099.1"
                     /translation="MEERVELRDLVREARVLIGRERARGRARIAVASAPVATTAASVA
                     APAVASLPASVPIAPREDVSRALSELAAAVAACRACSLCRTRTKTVFADGDPAARLLF
                     VGEAPGRDEDAQGLPFVGRAGQLLNKMIAAIDLKREGVYICNVLKCRPPDNRVPAPDE
                     VERCRPFLEQQIELVKPALICALGLSAAQALLQTKSSMASMRGKTFAFRGVPVIPTYH
                     PAALLRNPGLKREAWVDLQRVRDFLLQPGGGAAPGGTDGN"
     gene            complement(14304..15569)
                     /gene="coaBC"
                     /locus_tag="VFM00_02280"
     CDS             complement(14304..15569)
                     /gene="coaBC"
                     /locus_tag="VFM00_02280"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018951825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET7497100.1"
                     /translation="MAESGPVLTSARVLLGVTGGIAAYKAPELVRALAARGARVSVLL
                     TKNARRFVTVDALRAVTRGPVLCGLFEKEALGSGPWFEGAPPSPLGMAHIGMAREADL
                     VLVAPATANVIAKAALGLADDLLSTALLATRAPLLLAPAMNTAMWEHPAVQANVRALA
                     GRGASIVGPESGDLADGERGMGRMAPVDAIVEAAAAALARPSRLDGPLGGRVLVVTAG
                     GTEEPIDPVRVITNRSSGKMGIAIADEARRQGARVRLITARTSEMPPFGVERVLAMTA
                     SAMKEAVLDAMPEADALIMAAAVSDFAPAAATTEKIPRGNGGISLDLRPTEDILALVR
                     QRFPTKHLVGFALETHDETARGREKMKRKGLDLVAVNNPLRPGAAFGSDQNDVTLIGP
                     DGQPEPLGLRPKREVARAILARVATALAR"
     gene            complement(15574..15822)
                     /gene="rpoZ"
                     /locus_tag="VFM00_02285"
     CDS             complement(15574..15822)
                     /gene="rpoZ"
                     /locus_tag="VFM00_02285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET7497101.1"
                     /translation="MDNQANVLRSSNIPNKYELIIVAAREARRLNELSRQTGLNVGGK
                     VTNVALEEALKGNVLYRYADVPEDDEVKEESPAMPSDE"
     gene            complement(15863..16489)
                     /gene="gmk"
                     /locus_tag="VFM00_02290"
     CDS             complement(15863..16489)
                     /gene="gmk"
                     /locus_tag="VFM00_02290"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET7497102.1"
                     /translation="MNDPEAFLTDAYQRFVVVVSGPSGAGKSSFVKTLLEQGPDLVYS
                     VSATTRPRRPHEVEGHDYFFLAQDEFRRRVEAGEFVEHARVHGEWYGTLRSQIDDALR
                     RGKNVLLDIDVQGGKAVRNVYPDGVFIFILPPSMEALEARLRRRATDAEERIRLRLEN
                     ARREIPTVMEYDYSIVNDDFETASRRAVAIVWAERCRTRRREAVTAGS"
     gene            complement(16482..17366)
                     /locus_tag="VFM00_02295"
     CDS             complement(16482..17366)
                     /locus_tag="VFM00_02295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET7497103.1"
                     /translation="MTGYGRGEALTDGLRLAAEVRSVNHRFCEVSARLPRAFSVFEGE
                     ARKIVQDHVSRGKLTLVVSWEGGDESGEPGGALTLDERAAERYVELLRRAQARFGLKG
                     EIDLGTFALLPNVFTWEEAGRSSDFYLALLRDVVEKATRDLLRMKETEGEALKRDLVA
                     RAGLVRSRVARIRERAPDRVRETHEKMKERVRSLLSEIGETQIPEDRLLMEIALLSDR
                     LDCTEECVRLEAHCDHFLKFLDEESAPGRKLNFLLQEMNREINTIGSKSNDVAIVEQV
                     VEVKEELERIREQVQNIE"
     gene            17489..19273
                     /locus_tag="VFM00_02300"
     CDS             17489..19273
                     /locus_tag="VFM00_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497104.1"
                     /translation="MTPPAAANTQIPKRQSATRSPLLSVALGLAVLAAAVLPYLRTLD
                     FDFVWDDHYTVGTHLSIHGWSDVERLWKLPFDSLLNDVSLKRTYFRPATLYSLAADWA
                     GSPGDPRAFHRTNVVLYAAACLFLWLFASELTGRPVAAAAGAVIYALHPAHPESVAFI
                     SGRTDLLGGLFLYAAFWTALRFGPAIENPWWKLAPAALILLPSLYAKEIGLFAAPLIP
                     VALWVRDRKMRWGDVARTALPVALAGAVYLACRFAVLRASPLPTISPVEGTIPQILTS
                     VAVVARYLPLLFVPVGLSARHEIVETHAPDLVFFAGLLVLAGLAWGIARAARRRSPWL
                     IPLGLYAATLLPLCYVRLLSGAIVAERFLFVPSAALALAIALLPGVLTGRGAERAYLA
                     ACGGVALLFLVLLLPRVPLWKSDGTLFLSMLRDSPESPHVHAILGGYYYGKRDLPRAV
                     YHYRRAYALHPSTPDLLLNLGAAEDEMGQSDSAFAHIRLLNRVAPDYAPGLYALGNLY
                     VRIDRPDSAAAAYRASLARMPDFPQAENNLGAVLERQGKLDEALDHYRRAMAIDSTYP
                     DSRNNFRRLTAEIAASKSAKADGTRGAR"
     gene            19369..20775
                     /locus_tag="VFM00_02305"
     CDS             19369..20775
                     /locus_tag="VFM00_02305"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HET7497105.1"
                     /translation="MARAFLWIHMDRKEAALSLAPDLPMTPDPRGTRFGGLEGPLRGL
                     AIVEAELDPGALRLELAPDPDGEPAVELLVGTREEEAKRANLVLRSRGKGAEGAEETV
                     LFALHRGEHGQEEGTGASGAAGPSAAPPGFHPPRVRVAEEAAERETLRERIVRDFRED
                     FTREATKSLDRAEHSLARRVEALEGDRSRSSERILDRRKGEILLAHLHEIRRGANRVG
                     LPDPYADSPGSRIEIALDPSLSPHENAARLFRAAKRGERGAERIEARLRAAREALAKI
                     ADLRRVLQDRPAKEALEAIEAFHREAGVSGYRLRGDQREIHLGRRTALAPRAGRPGRP
                     SGAHHPIGPRTFTTSQGWEVWVGRNNTQNDQITHRLSNPHDYWLHVVGVPGSHVILRR
                     PSRNAIPKPSTLEEAASIAAYFSKARKLTRVPVIYTERKFVTRPRRGKPGQALCTRER
                     ELLVRPRLPEGDAGTESE"
     gene            complement(21371..23839)
                     /locus_tag="VFM00_02310"
     CDS             complement(21371..23839)
                     /locus_tag="VFM00_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7497106.1"
                     /translation="MDRTTAELIREREEAVSNARLTHDPERHLQSLEDLAEIFLRADS
                     YLPALQNLEECIRSAEALGLDAARIATLEMKAAQTLIEKGDSTAALRYLARARARVTG
                     PSQPELTARIALQSARALIERSEYDEAFRACEQAHTYFKERGLLAPLAHSYNCFGRIH
                     FRLGNLDQAKEYYEAALHLFRWDLNDEEGVVRAHNNLGILYRHLSDWRQASWHLLRSM
                     ELATRLGNFAYIAVSCANLGIVYLKAGSWDEARAHFDRALTSYVQIGRESGVARTRIG
                     LATIDIFRHDFAAAAAHIEAATQITSRLGASREQILCLFSRGEMKQEMGETDEALALY
                     DQALAMAREIAPEGDMIHELQRHRAEALALRGDLPEALEAAEDARDRASKLKDLLEEG
                     AARRVVAAIQTLAGSAPEAAAEAKESIRILEGIHERFELARAYLDQARRMPTRGDADR
                     MREAQNFAFKAMYLFEQLGIDDSLRAAEALLRDLNAPSWLTTPRAARREAGTGSSQAQ
                     EIARAHGIVTQNARMLELSRKASELLSTPARVLIQGETGAGKNLFAYMFRSYEVERGR
                     PFVEVNCTSLPGDLVESELFGYVRGAFTGAAITKRGIFEEADGGTIFLNEIGELTERT
                     QVKLLQVLDDGTYRRLGEVRSRRLNVRIISATNKDLDAAVKAGWFRADLYYRLGQVVL
                     SLPPLRERREDIALLIRSFLDELAVREGRQVVLSEDALEYLISLPWLGNIRELKHKVE
                     SIFLCAGRQELVDRGMLVKLLHPGGSPAAETHAARGLRSKVDLLKREEILAALSRNGG
                     NRSRAALELGITRRHLIRLLKQIY"
     gene            complement(23870..24205)
                     /locus_tag="VFM00_02315"
     CDS             complement(23870..24205)
                     /locus_tag="VFM00_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497107.1"
                     /translation="MGLLLPRVLRLAVLTLALLTLISLPTLRFDRNTGSALTPMIAWA
                     GGSPDETLKPPDSPPPSTPPKSAFITGFGTTSTDAATIGTHRVYTRLTALQRWQIFVS
                     VFRTFATRF"
     gene            complement(24344..26251)
                     /gene="selB"
                     /locus_tag="VFM00_02320"
     CDS             complement(24344..26251)
                     /gene="selB"
                     /locus_tag="VFM00_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HET7497108.1"
                     /translation="MRPVIVGTAGHIDHGKTALVRRLTGIDTDRLKEEKERGISIDLG
                     FAHLVLPSGARVGIVDVPGHERFVKNMLAGATGIDVALLVVAADEGVKPQTQEHLAIV
                     DLLGIRRGVVALTKSDLAPPERLARARDEVTALLRGTGLEGSPILPVSAVTGEGVAAL
                     LEALDQAAASAGAREAGRPARLPIDRIFSIEGIGTVVTGTLWSGTIRAGDSLQTLPSG
                     RSVRVRRVEVHDAEVVAAAAGQRTAVALHGAGREEIERGEWLVTPGGYRTAEALDVRL
                     TMLPGDPRERALATRTRLRIHLGASEVLGRAVLLEGERLEPGASAYAQLRLEHPVVAA
                     PGDRLVLRSYSPAVTIAGAVVIDPAPPRRSRLGGGDLERLKTLESGTLPEKVLWLARE
                     SGMEGMREEEAALRLGAGAEAVAAAAADGKALVRLKGGRTLFPDAWDRARGLVEARVR
                     EYAEAHPLRAGVPKGELKSLLSRDLDGGVFDEALASLLATGAVQVRGDRLLPPGAGPS
                     LSEADRQVLERIEQKLAGQGFQPPELSEVLRGVPRESRPTELMRHMIESGRAVKVTSE
                     LVYTRAQWEEIERRVAAHFARSPRLGMGDFKGYLQISRKYAVPLLEHLDRLGITRREG
                     DERIPGPKLKK"
     gene            complement(26280..>26860)
                     /locus_tag="VFM00_02325"
     CDS             complement(26280..>26860)
                     /locus_tag="VFM00_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET7497109.1"
                     /translation="TAMIPDRIEAGTLLAAAAITGGDVEVTNIDPSHLGAVLSKFETA
                     GCSVERGDRSARVRAPERPEALSVTTAPYPGFPTDMQAQFMALLSVGNGVSTLVDTIY
                     RDRFTHVAELQRLGADIRVDGNRALVHGVESLSGAPVMATDLRASAALILAGLVAGGE
                     TKVSRVYHLDRGYERLEEKLGRLGADIRRVRA"
BASE COUNT         3929 a         9479 c         9348 g         4104 t
ORIGIN      
        1 tcccgaaccg cgatcttcgg attcttctgc gacagcacca tcgtgatcgc cgccgtcaac
       61 gtcgtcttcc cgtgatccac gtggccaatc gtacccacgt tcacgtgcgg cttcgtccgc
      121 tcgtacttct gcttcgccat ccgatcagac cctccgctct gggttcgacg tcgatccggt
      181 ggaacgaccc cggcgcgatc aggactagga caccgatatc gagactgcga cgatcagcgc
      241 cgcggtcggc gcgccgttcg agttaatgga gcccacgagc ggaatcgaac cgctgacctt
      301 ttgcttacca tgcaaatgct ctaccgactg agctacgtgg gcggtggtgc ggcgcgcagc
      361 acgggccagg cgaggagagg gcatcgctcg ctccctcgag cgccggccct tcggcgcctg
      421 attgtttctg ataaggcaaa aaacgccgat cggccttcct cccgacttca agaaggtcgt
      481 cggtgactac gaatcgagta cggtaatcga gggcgaagat gcggtcaaca ggaaatttgc
      541 gccggttgtg ggtgccgctt cgggcctcga gtatttggac ttttgggggg tggaaaggat
      601 tctcgggtgg aggaaattgg ggggaatacg tgaaaatact tacgaattgg gtaagtgggt
      661 cgaactgtgg gcgttgggtt acttcacgtc gcggaggtct acgctgaaag gcccaggagt
      721 tccgccggct ctggccggcg gtacatgagt tccgccggac tgcggtccgg cggaatatgg
      781 agcgggaaac gggattcgaa cccgcgaccc tcagcttgga aggctgacgc tctagccact
      841 gagctattcc cgccccgaat cgttacttgc gactgcggtc gcctgccgct tgaccctttg
      901 actgctgaga aatggagggg gaaggattcg aaccttcgaa ggctaagccg gcagatttac
      961 agtctgctcc ctttgaccgc tcgggaaccc ctccggcacg gcgaatcgat cacgtttgga
     1021 gctggcgaag ggactcgaac ccccaaccgc ctcattacaa atgagatgct ctaccattga
     1081 gctacgccag caaagggacg cggacgcctg agcgcgcgcg ctccgggccg cagtgtacgg
     1141 ggagaggctc cggaggtcaa cgcgggaggg ggtgtcgagc cggccggggc gcaagcgggg
     1201 tcagcggcgc cgagctgtcg tcgattccga ccggagggcg gcaaggagcg agcccaccgg
     1261 aatctcgccg tcggcgcggt cgccgatcgg cgtgtccacc acgcgcgtga gcgtgaagtg
     1321 gaagcggctt ccctggccgg gcacgctctc ggcccacagc cgcccgcggt gcgcttcgat
     1381 gatgccgcgg cagatcgcca gccccagccc cgtgcctccc tgctcgcgcg tcgccgagtc
     1441 ttcgacctgc gtgaacttgc cgaagatccg ctcgagatcc tcgggcgcga tccccttccc
     1501 ctggtcgctc acgatcacct cgaactcgtg ctcgtgcgcg cgcagctcga tcgagacgcg
     1561 tccgcccggt ttggagaact tgagggcgtt ggagatcagg ttctcgagca cctgctggat
     1621 ccggctcggg tcggcgaaga ccatcgcggg ctccttgggg agccgggcgt ccacctcgat
     1681 cccctgtcct ccgggaagct gcggcaccca gaagaggcat ttctcgatca gctcgttcaa
     1741 ggacgtcgga aggaactgca ccgagagcac gccggcttcg atcttcgaga cgtcgagaat
     1801 gtccttcacc aggcggatca gccggtccac cgactcctga cagaaagcga tgagctggcg
     1861 ctggtcgctc gtctcgtcga atttgctgct ggagacgagg aggtcgagcg aggacttcat
     1921 cacggtgagc ggcgtgcgca cctcgtgaga ggcgatggag aggaactcgc tcttggagcg
     1981 gtccagctcc cgcacctggt cgaagaggcg cgcgttcccg accgcggccg ccacctgcgc
     2041 ggcgaagagc gaagcgaggg agacggcgtc ggagtggaac tgctcggcct cgccgaaggc
     2101 gaccagcgcg cccaccgtct cgtcgttcac ccgaaggggc gccgtcaggg cgtagccgcc
     2161 cgggatcgaa gcgaggcagg tgaatcccgc ggcggccagc tcggcgcggg tgaggagccg
     2221 cgtctcggcg ccgagcacct tctccaccgg ctcgagcgcg acctcccact catcggcgga
     2281 gaccgcgcgc ccctccacgg cccagacgcg tatggcgcgg tcgcggcgcc ccgcggcgcc
     2341 ggccacgacg acgtcgccgc cgcagatcgc ccgcaggccg cgcgccgcca cggcgagcat
     2401 ccgcgggagc tccagcgtcg ccgccgtctg cgagccgagc cggtggaggt cgcggaggcg
     2461 cgcctgctgc gcggcgttcc gcgactgcga ggcctccagg ctctgcgcca tgagattgat
     2521 cgcgtccccc agctgcccca gctcgttgcg cgcgcccacc tggatccgcg tctcgagcct
     2581 cccgcgcgcg atctcgccga ccccgcgcag cagctcatcc accggccggg tgacccagcg
     2641 ggagacggcg ctcgaggccg cgacgctcgc gcccagcccc acgaggaaga tcccgagcgt
     2701 gacgtaccag aggaggtggg cctcggcgat gagccccgcc gcgctcagct ccacgacgac
     2761 cagtccgacc gactcgccgg aatcggtgcg gatcggcgcg accgccacga cgcgcgccat
     2821 gtccggcttc gtgagatcca ggatcgcgca ggccgtgctg gtcatggcgg cgtgaatcag
     2881 gtcggcgggg acggtctccg gcggcggcga gccgatcggg aggcggccgc cgcgaagcga
     2941 ggtctcctcg cggaccggcg cggcgccgcc catgtcgagg accacgcccc aggcgccgag
     3001 cctgccgacg ggcgccagcg tgaagaggtg gtagatcgag ggattcgcgc gccggagcgc
     3061 gctcaggtcg tccacgatgg tgcggaaggc ctgcgtgttc atgtcggcgg gcgttcggat
     3121 caggcggtgg tcgtcggcgt ggatcagcgc ggcggcggtc tcggcggcgg tggtcgcggt
     3181 gctggccagc tggcggcgca aggcggcctg gccgttctga tacagcaaga tcgtggcgag
     3241 gacgaggaag ccgctgccca cggcgaggaa caacatctga agttcgccct tgagcgttcg
     3301 cgggcgttgg aagagctgca tgggcggcgc cgtgccctcg aaggagttac gtccggcgcc
     3361 ggaccttcgt cccctccttg gagtccttca ggtcgtatcc cagcgccgcg atctggtccc
     3421 tcaattgatc ggcacgcccc cactgcatct gcatcctggc ctcatcccgt tgccgtacaa
     3481 gctcttgcac ctcgaggggg agggcctgcg cggcgggcgg cccgccccag aagaggccga
     3541 tcgtctcccc cagctcccgg agatggcgcc cggcgacggg cagggcggcg cgctcctgcg
     3601 gatgggcgtc cgcctggtcg gccacgcgat tgatcgcctt ggcgatctcg aagaggtgtc
     3661 cctgagcgcg cgcggtgttg aagtcgtcgt ccatcgcctc gccgaactgg cggcgcagct
     3721 cggaaatttc ctgtaagagg cgggtcgcgt cgaggtcctg cccctgcccc gccgcggagg
     3781 cgccggcgct ttccgcccgc gcgagcgcat cgcgcagccg cgcgtaggcg atccccgcct
     3841 cttccagccg ctcctcgttc cactcgatcg ggctccggta gtgcgtggac tggaggtaga
     3901 accgcaccac ctcgggctcg acgcgcgcga ccacatcctc gatcgagaag aagtgcttcg
     3961 tcgacttgga catcttcgcg ccttccagat tcacgaggcc gttctcgatc cagtagcgcg
     4021 cgaaggtgcg gccggtggcg gcctccgact gcgcgatctc gttctcgtgg tgcgggaaga
     4081 ccaggtcctg gccgccgccg tggaagtcga actcctcgcc caggtacttc atcgccatcg
     4141 ccgagcactc gatgtgccac cccggccggc ccggacccca gggactgtcc cacgcgggct
     4201 cgcccggctt ggcgcccttc cagagcgtga agtcgagcgg atcgcgcttg gcctcgccga
     4261 cctcgatccg gacgccgctc cggagctcgt ccacgttcct ccccgacagc tcgccatagc
     4321 gcggccagcg gcgcacgtcg aagtagacgt cgccgttcga cgcgtacgcg tgacccttct
     4381 cgatgagcgt cgcgatcagc gccgtgatct cgggaatgtg gcgtgtcgct ctcgggtagt
     4441 gcgtcgcggg cttcaggttc aggagctcgc cgtagcggag gaacgcctgg atgttccgct
     4501 ccgcgacctc gccgtgcgga atcccctcct cgttcgcgcg cgcgatgatg cggtcgtcga
     4561 tgtcggtgaa gttcgtgacg taggtgacca cgaagccctt cgactcgaga tagcggcgga
     4621 tcgcgtcggt ggagacggcg tagcgcatgt ggccgacgtg cggcttgtcc tggacggtca
     4681 tgccgcagac gtacatgccc gcgcgccccg gaaggaccgg atcgtacgga accttggacc
     4741 gccggcgcgt gtcgtagacg cggatcccgg ctcccggcgt gccgctcatg gccggcgctc
     4801 catccggctc ccgctcacgc gccgtccgcc gccgcgtcgt ggaggcgcgc gtcgcgcagc
     4861 ttcgcggccg acccgggcac cgtgccgggc gcgggcgatt cggccgcggt cgagtggatc
     4921 gtgatcccct cggcgcgaag ccgcgtgacc gcgccgaagc gaagcgcgcg cgccacggcg
     4981 aggcggtgct cgggaagggt tcgcgcctgg agcatcatcg tgtactgccc gaccgagacg
     5041 tcctccatcc cgaggacgcg cggcgcgtcg cggaggagcg gcttcagcgc gggctccagc
     5101 gcggcctcgg ccaggtagcg ctccaggacc gacgcgacgc gggccggatc ctccgtcagg
     5161 ctgaccggga tccgcacgat gggcgacgac caggccttgg tcagattggt caccaccttg
     5221 atctctccgt tgggcacgaa gtgcagagct ccctcgccgt cccggacctc ggtgtggtgc
     5281 agcgtgagcg cctcgacggt gccggccacg cccgctacct gcaccacgtc gccgaccacg
     5341 tactggttgt cgtagatgat gaagaagccg ctgatccagt cgcgcaccaa ggactgcgcg
     5401 ccgagccccg cggcgacgcc gaagccgccg gcggtcgcga tcgccggcgt gatgtccagc
     5461 cccaactccc gcaccgccat cagcagcgcg accacgatga tcgcggcccg cgccagcccg
     5521 cgcaccacgc cgcccagcgt gcgcgcgcgc ttctcgcgcg agatctcgtc cccccggccc
     5581 gcggcgcgaa tcgcccgctc caggcgctgg acgaggatcc ccgccacccg gatcaggatg
     5641 agcgcgccga gcgcgatcag cccgactttg atcgcgagcg tggcgtagtt gaacgcttcg
     5701 gcggcgtcgg cgcggaacgg cgtgggtctc acggggtcgt cgggatcgtc gacgtcatcg
     5761 cgatggggcg aacgggagcc ggcgtcatgc ggccggtctc aggcgccgga gcgcgcggcc
     5821 gtgcgggccg gcgccgattc cacgatggcg cgcacgagat cccggtagga gagtcccgcc
     5881 gcgccggccg ccttgggaac gaggctggtc tcggtcatgc cgggcacggt gttcacctcg
     5941 aggcaccacg gcgtgccgtc ctcggccaga cggaagtcga cgcgcgcgat gccggagcag
     6001 cggagcgccc ggtaggattc gcgcgccagc gcgagcaggc gctcggtcag gtcgggcgga
     6061 agcttggcgg gcacctcgta gcggctggcg ccggccgtgt acttgtgctg gtagtcgtac
     6121 cagccccctt cgggggtgat ctcgaccacc ggaaaggcac gctcgtccag caccgccacc
     6181 gtcacctcgc gtccttcgat gaacgactcg accaggatca ggcggctgta gcggcgcgcc
     6241 tcttcgatcg cgggaaggag atcctcgcga cgcttggcca cggagattcc gacgctcgag
     6301 ccctggtcgt tcggcttcac gatgaggggg aagccgccga gcctggcgat ggccgcctcg
     6361 ccgatcgccg gcgcccaggc cgctgcggga tcggcgggcg cctcgagctc gatccagtcg
     6421 ggggtgggaa tgccgaagtc gcggaagatc cgcttcgaga gcgatttgtc catcgagacg
     6481 gccgacgcga ggacgcccga cccggtgtag gggatgcggg cgaggtcgag caccgcctgc
     6541 agcaccccgt tctcgccgtc gccgccgtgc aaggcgatga atacgaggtc ggccgcgcgc
     6601 gggatcgctt tcgcgtagat ctcgagattg cccgcgcccg cgccgggaac gggaagccgg
     6661 ccggaatcgt cgagcgattc cgcgggggcc tcggccggcg gcgccgcgcg ccccgagccc
     6721 agggcctgcg ccgcgtcggg atcggggagg atccggccgg tgccggtatc gagcagcgtg
     6781 acctcgtgcc ccagatcgag cagcgcgcgg ccgatgcccg cgccgcttcg gagcgagact
     6841 tcgcgctcgg gggtgttccc tcccaacagc acgaccacct tcatggggtc cggtcctccc
     6901 tggggatgct caggggcgat ccacctcgac cagcgccacg gccatggcgg cgatcccctc
     6961 gccgcggccg atggggccca gcccttcgtt tgtcgtcgcc ttgaccgaca cgcggtcggc
     7021 gccgaccgcg agcgccctcg ccagattgtc ccgcatcgcg gcgacgtggg gcgcgatctt
     7081 cggcgcctcg gcgattacgg tagcgtccac gttcaccacg cggccgccgc gcgatgccgc
     7141 gagctcgcgg atccgttcga ggagatcgag cgaggaggcc ccgcgccacc gggaatcggt
     7201 atcgggaaaa tggctgccga gatcgcccag cccgagcgcg cccaagatgg cgtcgccgat
     7261 cgcgtgcgcg agcgcgtcgc cgtccgagtg ggcgcgaagc cccttctcga acgcgagcgc
     7321 cacgccgccc agaaccagcg gcacgccgcg ctccaggcga tggacgtcgt agccgttccc
     7381 gacccgcagt tcccgatcca cgtcagacct ccccgctcga tcgtaaacgg aggagcgcca
     7441 aggccacgat cagatcggtc ggttccgtga tcttcagatt ggacggctcg cccgcgacca
     7501 cgtggacggg caatccgagc gcctcgacca gcgccgcgtc gtcggtggcg tcgagagtcg
     7561 tccgttcctg ggcgtgggcg cgcgcgagta tagcacgcga aaaaatctgc ggcgtctgcg
     7621 cgccgacgag cttctcgcgc gggacggagc gcgtcacgcg accgtcctcc acctccttca
     7681 ccgtgtcccg gatcgggagc gcggggatcg ccgcgccgtg ctcgcgcgcc gcggcgaaca
     7741 cgcgctcgat cagcgcggcg gtcgcgagcg gccgcgcgac gtcgtgcacg gcgacgtagg
     7801 aggccctcgg atcgaggctc tccagcccca gccgcaccga ctcctggcgc gtggcgcctc
     7861 ccgccagcgc gcgggctccc accacgcgct cccgcggcac ttgcgccagc gcgtcgcgcg
     7921 ccggcccgag cagcgcttcg gggacgaccg cgacgatctc ggtcacggcg gcgtgcgcga
     7981 ggaacggcgc ggccgcgcgc tcgagcagcg tctttcccgc gaggtcgata aggagcttgg
     8041 ggccgcgtcc ttccatccgg ctgcccgatc cggcgcccgc gaggatcacc gccagctcca
     8101 cgcgcgcctc aggtggccag cgtccgctcg atggcctcgg ccagggtagc ggacggaagg
     8161 agcgcgatcc ccgagggcgg cgcgtcggcc gcgtccaggg agcgcttcgg cacgatcgcc
     8221 gagcggaagc cgagtcgcgc ggcctcggcc aggcgccgcg aaagctgcgg caccggacgg
     8281 agctcgccgc ccaggcccac ctcgccgatc atgacgcgat cgggcgggag cggccggtcg
     8341 cggaacgagg aggccagcgc cagggccagg ccgagatcgg cggcgggctc gtcgaggcgg
     8401 atgccgccgg ccacgttcac gaagacgtcg gcgcccgcga gcgagaggcg cgcgcgcttc
     8461 tccagcacgg cgatcacgac ggcgaggcgc ttgacgtcca cgccctgcgc cacccgctgc
     8521 ggattggcga agtgggtggg cgcgaccagc gcctggactt cgaccaggag cgggcgcgtc
     8581 ccctcgaccg acgcgaccac ggcggagccg ggcaccggct ccccgcgctc gcgcaggagc
     8641 cgctccgagg gattcgtgac ctcgatcagc ccgtcgcccc gcatctcgaa gacgccgatc
     8701 tcgtgggtcg agccgaatcg gttcttggcc gcgctcagga tgcggtagcc gtggtgcgcg
     8761 tccccctcga gatacagcac cgcgtccacc atgtgctcga gggtgcgcgg ccccgcgacc
     8821 gtcccatcct tggtgacgtg gccgatcagg agcaccggga ggccgcgctc cttggccagg
     8881 cgcaacaggc ggagcccgca ctcgcgcacc tgggagacgc tccccggcgc ggcgtcgagc
     8941 gtggcgaggg agagggtctg cacggagtcg acgacgagga gggcagggtt caagcgcgcc
     9001 acttcggcct cgatggcttc gaggtcgacc tcggcgtgga tccagagatt ctcggatttc
     9061 ccgagcagcg cggtccgctc ggcgcgcatg cgcgtctggc gcggtgattc ctcgccggag
     9121 acgtagagga ccgaccgccc cgccgcgccc agcgccgccg ccatctccag cgcgagggtg
     9181 gattttccga tcccgggatc gccgccgatc agaacgagcg agcccgggac cagcccgccg
     9241 ccaagcacgc ggtccagttc ggcgatgccc gtgcgccagc ggtcctcggc gcccgtctcg
     9301 atcgcctcga ggcgcaccgg ggccgacggc gcgacgccgg catatcccgc cgcgcgccgc
     9361 cccgcgccgg tcgcgcgccg cgcggcgccc cccgcgggag cctccgtggc gctgttccac
     9421 gccccgcagt gcgcgcattt cccgaaccac ttcgggcttt cgttgccgca ctcggcgcag
     9481 aagaagggcc ccgcggccgc gcgtgcccgg gacgtttcct tacgcgccac gaccgctccg
     9541 gcgggaccgc accatgtcag aagagtccga tcttgagata gcgcttcgga ttcttccgga
     9601 tgtccttcac cagctcgttc gtgttccgga ttgtctcgcg catctcggta tagagggtcg
     9661 tgtcggcgat ggcgcggccc accgagccct cgttcgactc caccttcgcg gccacgcgct
     9721 gaagcgacgc cgcggtcgcc gagagctggt cggccaggcc ggagagcttg cgcgaggcgg
     9781 tgtcgaagtt ctggagcgac gtgttcacct ggtccttgtt ctgggacgcg atggcgcgca
     9841 ggtcgtgcgc cgccgccttg aagtcgagga tcgcctggcg caggtcctgg cggttgtcgc
     9901 cgagcgccga attgagcgag ccggaggcgc gctccatgtt cttcgacgtg cgctccacca
     9961 ccgcggggtc gagggcgtcg agcatcacgt tgagccgcga ggagagctcg gtcgcctcga
    10021 tcaggagatc gcccgcgccg gagatgacct cgggaacgcc cgactggaag cggccgggga
    10081 tcggcttcgt gtggtcgtat cgccccggcg ccgtcccggg ctcgagggcg aggaacttct
    10141 cccccatgaa cccgatgttc gcgatccgga gcgaggcgtc gggatggagg atgacgtccc
    10201 ggtccagctc cagctccacg cgcaccgcct ggtcctccag cttgacgtcg gtgacggcac
    10261 ccttgcggac gccgcgcacg ttgaccggat ctccctcggc caggtttccg acctcctgga
    10321 agcgggcggt gtaggtcatc ttcttcttcc ccagccggta ctccttgatc cagatcacgc
    10381 ccgcgagcag cagcgccagg gcggccagcc ccatgattcc gacctgcacc gcgttcctgc
    10441 gcgatggatt catccggccc ccgtcatgtg ggaaggagcg gcccctcgga ggacccctcg
    10501 atgaactgcc gcagcgccgg gtcggtggtc gcctggatct cgtccggcgt tcccatggcg
    10561 tggatccgcc cctcgtgcag catggcgatg cggtcggcga tatgataggc gctccgcatg
    10621 tcgtgcgtca cggcgatcga cgtcatgccc agacgctgct ggaggcgccg gatgagctgg
    10681 ttgatcaggt ccgacgtgat cggatcgagc cccgtcgtcg gctcgtcgta gagcatgatc
    10741 ttgggattca gcacgaggga gcgggcgagc gccgcgcgct tcttcatccc gccgctcaag
    10801 ctggaggggc gcatccgctc gcttccctcg agccccacca gcgcgagctt ttccgagacg
    10861 acgatctcga tctgggtctc gggcatcggc gtgtgctcgc ggagcggcag ccccacgttc
    10921 tcgccgatcg tcatcgagtc gaacagagcg cccccctgga agagcatccc catgccgcgc
    10981 cgaagctcga gcagctcctt ctcggggaga tgctccacct ccatcccgtt cacgcagacg
    11041 tgcccccggt cgggtgccaa aagcccgacg acgtgcttca ggagcaccga tttcccggtg
    11101 ccgctccgcc cgatgatgac catgcattcg ccgcggcgca cctcgaggtt cagaccccgg
    11161 agcacgtcct tccggccgag ccgcttccag acgtcctgga actcgatcgc ggcgtccgat
    11221 cgcggcccgc cacccaactc ccgctctggc ccccggtcag gcaaaaatca cccggaagag
    11281 gaacgaggcg agcaagtagt ccacgatcag gatgagaaga caggaggcca cgacggcgtg
    11341 cgtggtcgcc acgcccacgc cctcggcgcc cccctcgctc ttcagcccga agtagcaccc
    11401 catcatcgcg atgatgcctc cgaacgcgaa cgacttgatg agcccggaga agacgtcctg
    11461 gatctggaag aagagcctca agccgctcac gaacgtgtgc gaggagacgc ccatcgtgag
    11521 gaccgcgacg agccacccgc cgaagatcgc cacgatgtcg gcgaagatgg tgagcgcggg
    11581 gagcatgagg accgcggcca aaaagcgcgg cacgacgagg aagcgggtgg ggctgatgcc
    11641 catcgtctcc atggcgtcca cctgctctgt gacgcgcatg gtgcccagct cggccgcgat
    11701 cgaggcgccg acccggccgc ccacgacgag cgcggtgagg accggtccca gctcgatcac
    11761 caccgacttc ccgatcaccg tgcccaggta gagcatcggc acgtagtcgc gcatctggta
    11821 gagcgcctgc accgccgtca ccgcacccgt gaagagcgcg acgatgacgg tgagcggcat
    11881 cgagcccacg ccgatgtgcg ccatctggtc gatgatcagc ccggggcggg ccagcgcggc
    11941 cgggatctgc cggtggacct tgccgaccag gatgaaaacc tcgccgacgt cgcccagcca
    12001 ccccatggcg acgcgctccg gccaggtgtt ctgcggcggc tcggcgggtt cggtcaaggc
    12061 gatgcgtgga ctccgtcgcg gacgcgcggg cgcgccggac tagacatggg gtccgcgcgg
    12121 ctcgggcacg ttccgcagcg agtcgaatcg cgtgcactcg ccgaggaacg ccagatccac
    12181 gcttccggtc gggccgtttc gctgcttggc gatgatgatc tccgccttgc cgtccttctt
    12241 gtcgggctcg gtctcgtaga tgtcgtggcg gtagacgaag aggaccacgt cggcgtcctg
    12301 ctcgatcgcg cccgactcgc ggagatcgga gagcatggga cgcttgtcac cgccccgcat
    12361 ctcgacggcg cgcgagagct gcgagagggc gaccaccggg acgttcagct ccttcgccat
    12421 gatcttgagc gagcgcgaga tcgcggacat ctcctgctgg cggttctcgg cccgcgaggg
    12481 gccgcgaacc atctgcatgt agtctacgaa gatgatgccg aggtcgccgg cggcggccag
    12541 gcggcgcgcc ttggccttca tctcgaggat gccgagcgag gggctgtcgt cgatgtagat
    12601 cggcgcctgg tggagctggg cggccgcgtt ctggagccgc ttgtactcgt cgtagctcgc
    12661 gtacccgcgg cggatcttgt ggatgtcgac gcgcgccatc gagcagagga gccgctgcac
    12721 cagggactcc ttggacatct ccaggctgaa gatcgcgacc ccgggaccgg gcttcccgcg
    12781 cgtccccacc gcgacgttct cggcgatgtt gagggcgaac gaggtcttgc ccatcgaggg
    12841 gcggccggcg atcacgacga ggtcggcccg ctggaacccc gcggtcatct cgtcgagatc
    12901 ggcgtatccg ctcgggatcc cggtcacgtg gacctttcgc ttccggtact cgtcgatggc
    12961 ctcgaacgcg gccagcacca ggcggttcat cggcacaaag ccctggcgga gctggtcgtt
    13021 cgcgatctcg aagatccgct gctcggagcg gtcgagcagg ttctccacct cctcggcctc
    13081 cgcgaacgcc tcgtcggaga tggtgtgccc cgcctcgagc agccggcgga gcgtccactt
    13141 gtcgaggacg atcttgcagt agtgctccac gtgccccggc gaggcgacgt tctggtcgag
    13201 cgacgtgaga tagctgtagc cgccgcacgc ctccagctgg ttccgcttcc gcagctcctc
    13261 cgacaccgtg accaggtcga tcgcggcgtt cttctcgtag agcgccatca tcgcggtcca
    13321 gatctcgcgg tgccgcggga cgtagaaagc gtccttgctg cgcacgatct cgacggcgtg
    13381 gcccaccgcg tcccgggaga ggagcatgga gccgaggatc gccttctcgg cttcctcggc
    13441 ctggggcggg acgcgcggcc cttccacctt gagcgcgggc gcgggcgcgg cgccgccgaa
    13501 cgcgcgccgg gggtcagttg ccatcggttc ctcccggcgc cgcgccgccg ccaggctgga
    13561 gcaggaagtc gcgcacgcgc tggagatcca cccacgcctc gcgcttcaag cccggattcc
    13621 ggaggagcgc ggcggggtgg tacgtcggaa tcaccggaac gccccggaac gcgaacgtct
    13681 tcccacgcat cgaggccatc gaggacttgg tctggaggag cgcctgcgcc gcggagagcc
    13741 cgagcgcgca gatcagggcc ggcttcacca gttcgatctg ctgctcgagg aaggggcggc
    13801 agcgctccac ttcgtcggga gcggggacgc ggttgtcggg cgggcggcac ttgagcacgt
    13861 tgcagatgta gacgccttcg cgcttgaggt cgatcgccgc gatcatcttg ttgaggagct
    13921 gccccgcccg gcccacgaac ggaagtccct gcgcgtcctc gtcacggccc ggagcctcgc
    13981 ccacgaagag gaggcgcgcc gcgggatccc cgtcggcgaa gacggtcttc gtgcgggtgc
    14041 ggcagaggga gcaggcgcgg caggcggcga cggcggcggc gagctcggag agcgcccgcg
    14101 agacgtcctc gcgcggggcg atggggacgg acgctgggag ggatgcgacc gccggcgcgg
    14161 ccacggacgc ggccgtagtg gccaccgggg ccgacgcgac cgcgatgcgc gcgcgcccgc
    14221 gtgcccgctc gcgcccgatc aggacgcgcg cttcccgcac caggtcccgg agctccaccc
    14281 tctcctccac cgccacaccc tcctcagcgc gccagcgccg tcgccacgcg cgccaggatc
    14341 gcgcgcgcca cctcccgctt gggacggagc ccgagaggct cgggctggcc gtccggtcca
    14401 atcagcgtca cgtcgttctg gtccgacccg aacgccgccc ccggacggag ggggttgttg
    14461 acggcgacca ggtccagccc tttccgcttc atcttctcgc gcccgcgcgc ggtctcgtcg
    14521 tgcgtctcga gcgcgaatcc caccaggtgc ttcgtgggga accgctggcg caccagcgcc
    14581 aggatgtcct ccgtgggacg gaggtccagg gagatcccgc cgttgccgcg gggaatcttc
    14641 tccgtcgtgg ccgcggccgg agcgaaatcg ctcacggccg ccgccatgat caaggcgtcc
    14701 gcttcgggca tcgcgtccag cacggcttcc ttcatcgccg aggcggtcat cgccaggacg
    14761 cgctccacgc cgaacggcgg catctcggac gtgcgcgccg tgatcagccg cacccgcgcg
    14821 ccctgccggc gcgcctcgtc cgcgatggcg atgcccatct tcccgctcga gcggttcgtg
    14881 atcacgcgga cgggatcgat cggctcttcg gtcccgcccg ccgtcacgac gagcacgcgt
    14941 ccccccagcg ggccgtccag gcgcgagggg cgcgcgagcg cggccgcggc cgcctcgacg
    15001 atcgcgtcga cgggggccat ccgtcccatg ccgcgctcgc cgtccgccag gtcgccgctc
    15061 tcgggcccca cgatcgaggc gccgcgaccg gccagcgcgc ggacgttcgc ctgcaccgcg
    15121 gggtgctccc acatcgcggt gttcatcgcg ggggccagga ggagcggagc gcgcgtggcg
    15181 aggagtgccg tggagaggag gtcgtcggcc aggcccagcg ccgccttcgc gatcacgttg
    15241 gcggtggcgg gggcgacgag aaccaggtcg gcctcgcgcg ccatgccgat gtgggccatg
    15301 ccgagcggcg aagggggcgc gccctcgaac caggggcccg agccgagcgc ctctttctcg
    15361 aagagcccgc agaggaccgg cccgcgcgtc acggcgcgca gcgcgtccac ggtgacgaag
    15421 cgccgggcgt tcttcgtgag caggaccgag acccgggccc cgcgcgccgc gagggcgcgc
    15481 accagctccg gagccttgta ggcggcgatg cccccggtca cgccgaggag cacgcgcgcg
    15541 ctggtcagga ccggtccgga ctccgccacg acgtcactcg tccgacggca tcgccggcga
    15601 ttcttccttc acctcgtcgt cttcggggac gtcggcgtag cggtagagga cgtttccctt
    15661 gagggcttcc tccagggcga cgttcgtcac tttgccgccg acgttcagac cggtctggcg
    15721 cgagagctcg ttcagccggc gcgcctcgcg ggcggccacg atgatgagct cgtacttgtt
    15781 ggggatgttg ctggatcgga gcacgttggc ctggttgtcc atgaagtcct cctgcgaata
    15841 gagtagcgcg gcttgggccc cgctacgagc ccgccgtgac cgcttcgcga cgccgggtcc
    15901 ggcaccgctc ggcccagacg atcgcgaccg cccggcggga cgcggtctcg aagtcgtcgt
    15961 tcacgatcga atagtcgtat tccatcaccg tcgggatctc gcgccgcgcg ttctccagtc
    16021 gcagccggat ccgctcctcg gcatcggtgg cgcgtcgccg aagacgcgct tccagggcct
    16081 ccatcgaggg aggcaggatg aagatgaaca ccccgtcggg gtagacgttc cggaccgcct
    16141 tccccccctg cacgtcgatg tccaggagca cgttcttgcc ccgacgaagc gcgtcgtcga
    16201 tctgcgagcg aagcgtcccg taccactcgc cgtggacgcg ggcgtgctcc acgaactcgc
    16261 ccgcctccac ccgccgccgg aactcgtcct gcgccaggaa gaagtagtcg tgcccctcga
    16321 cctcgtgcgg ccgccgcggc cgggtggtcg ccgacaccga gtagaccagg tcgggcccct
    16381 gctccagcag cgtcttcacg aaggagctct tcccggcgcc cgagggcccc gacacgacga
    16441 ccacgaaccg ctggtatgcg tcggtcagga acgcttccgg atcattcaat gttctggacc
    16501 tgctcgcgga tccgctcgag ctcctccttg acctcgacca cctgctccac gatcgccacg
    16561 tcgttcgact tgctgccgat cgtgttgatc tcccggttca tctcctggag caggaagttg
    16621 agcttcctcc ccggagccga ctcttcgtcc aggaacttga ggaagtggtc gcagtgcgct
    16681 tccagccgga cgcactcctc ggtgcagtcg agccggtcgg acaggagcgc gatctccatg
    16741 aggagccggt cctcggggat ctgcgtctcc ccgatctccg agaggaggga gcgcacgcgc
    16801 tccttcatct tctcgtgcgt ctcgcgcacc cggtcggggg cgcgctcgcg gatccgcgcg
    16861 acgcggctcc gcaccagccc ggcgcgcgcg accaggtccc gcttcagcgc ctccccctcg
    16921 gtctccttca tgcggagcag gtcgcgggtc gccttctcga cgacatcgcg caggagcgcc
    16981 aggtagaagt ccgacgagcg ccccgcctct tcccacgtga agacgttggg caacaacgcg
    17041 aaggtgccga gatcgatctc acccttgagc ccgaaccgag cctgcgcccg ccggagcagc
    17101 tcgacatagc gctccgccgc gcgctcatcc agggtgaggg cgccccccgg ctcgcccgat
    17161 tcgtcgcccc cttcccagga cacgacgagg gtcagcttgc ccctcgagac gtggtcctgg
    17221 acgatcttcc gggcctcccc ctcgaacacg gagaaggcgc ggggcagacg cgccgaaact
    17281 tcgcagaatc ggtggtttac ggatcggact tcggccgcga gacggaggcc gtcggtcagg
    17341 gcctcacccc ggccgtaacc ggtcatgctt cggaccatcg tggtggtacc gtcccccgtg
    17401 gtaggcgggt actctacccc tcggcggccc ccaggtcaac gaagcgaggg cttgaccgtg
    17461 ccgcgatccg ctccctaacg tgccgcccat gacgccgcct gccgccgcca acacgcaaat
    17521 cccgaagcgg cagagcgcta cccgctcgcc gcttttgtcg gtggcgctgg gcctggcggt
    17581 gctggcggcg gcggtcctcc cctacctgcg cacgctcgac ttcgacttcg tctgggacga
    17641 ccactacacg gtcggaacgc acctttccat ccacggctgg agcgacgtgg agcgcctctg
    17701 gaagctcccc ttcgactcgc tcctgaacga cgtctcgctg aaacggacct acttccgccc
    17761 cgcgacgctc tacagcctgg ccgcggactg ggcgggttcg ccgggagacc cgcgcgcctt
    17821 ccatcgaacg aacgtggtcc tctacgccgc cgcctgtctc ttcctctggc tcttcgcgtc
    17881 ggagcttacc ggccgtccgg tggcggccgc cgcgggcgcc gtgatctacg ccctgcaccc
    17941 ggcgcacccc gagagcgtcg ccttcatctc cgggcggacc gacctcctgg gcgggctctt
    18001 cctctacgcc gcgttctgga cggcgctccg cttcgggccc gccatcgaga acccctggtg
    18061 gaagctcgcg ccggcggccc tgatcctcct tccgagcctc tacgccaagg agatcggcct
    18121 cttcgcggca cccctgatcc cggtggctct ctgggtgcgc gaccggaaga tgcgctgggg
    18181 cgatgtcgcg cgcacagcgc ttcccgtggc cctggccggc gccgtctacc tcgcgtgccg
    18241 cttcgccgtg ctgcgcgcct cgcccctgcc gaccatctcg ccggtggagg ggacgattcc
    18301 ccagatcctg acgagcgtcg cggtggtcgc gcgctacctg cccctgctgt tcgtcccggt
    18361 cgggctctcg gcccgccacg agatcgtgga gacccatgcg cccgacctgg tcttcttcgc
    18421 cgggctcctg gtcctcgccg gactcgcctg ggggatcgcg cgggccgcgc ggcggcgctc
    18481 cccctggctg atcccgctcg ggctctatgc ggccacgctg cttccgctct gctacgtgcg
    18541 cctcctttcg ggagcgatcg tggccgagcg attcctcttc gttcccagcg ccgcgctcgc
    18601 gctcgcgatc gcgctccttc ccggggtcct gacgggccgc ggcgcggagc gcgcctacct
    18661 ggccgcgtgc ggcggggtgg cgctcctgtt cctggtcctc ctccttccgc gcgtcccgct
    18721 ctggaagagc gacggcacgc tctttctctc gatgctccgc gattctcccg agtcgccgca
    18781 cgtgcacgcg atcctgggcg gctactacta cggaaagcgg gacctccctc gcgcggtcta
    18841 ccactaccgg cgcgcgtacg cgctccaccc ctcgacgccc gacctgctct tgaacctggg
    18901 ggccgccgag gacgagatgg gccagagcga ctccgcgttc gcccacatcc ggctcctgaa
    18961 ccgcgtcgct ccggactacg cgccggggct ctacgcgctc gggaacctct acgtgcggat
    19021 cgaccgcccc gactcggcgg cggccgccta tcgcgcgtcg ctcgcgcgca tgccggactt
    19081 tccgcaggcc gagaacaacc tgggcgcggt gctcgagcgc caggggaagc tggacgaggc
    19141 gctggaccac taccggcgcg cgatggccat cgactcgacc taccccgact cccggaacaa
    19201 cttcagacgt ctgacggccg agatcgccgc ttccaagagc gcgaaagcgg acggaacgcg
    19261 gggggcccgc tgagctccct tccggaatcg ctccggggac gaacgatcac cggggtcgtc
    19321 ccgctcggac cccgctcgct cgcgctcggg ctggctccgg ccggtgccgt ggcgcgggcc
    19381 tttctctgga tccacatgga ccgcaaggaa gccgcgctca gcctggcgcc cgatctcccc
    19441 atgacgcccg atccccgcgg gactcgcttc ggtgggctcg agggcccgct gcgcggcctc
    19501 gcgatcgtgg aggcggagct ggacccgggg gcgctccggc tggagctcgc ccccgatccg
    19561 gacggggagc cggccgtcga gctgctcgtg ggaacgcgcg aggaggaggc gaagcgcgcg
    19621 aatctcgtcc tgcgttcgag aggcaaggga gccgagggcg cagaggaaac ggtgctcttc
    19681 gcgctccacc gcggggagca cggccaagaa gaagggaccg gcgcgtccgg cgcggcaggc
    19741 ccttccgcgg cgccgcccgg gtttcacccc ccgcgtgtcc gcgtcgccga ggaggcggcg
    19801 gagcgggaga cgcttcgcga acggatcgtg cgggacttcc gggaggattt cacgcgcgag
    19861 gcgacgaaga gcctcgatcg cgccgagcac tccctggctc ggcgcgtcga ggcgttggag
    19921 ggggaccgaa gccgctcgag cgagcggatc ctcgaccggc gcaagggcga gatcctgctc
    19981 gcgcatctgc atgagattcg gaggggggcg aaccgcgtcg gactccccga tccatacgcg
    20041 gactcgccag gctcccggat cgaaatcgcg ctcgatcctt cgctctcccc tcacgagaac
    20101 gcggcgcggc tctttcgggc cgcgaagcga ggcgaacgcg gagcggagcg aatcgaggca
    20161 aggctccgag ccgcgcgcga ggcgctggcg aagatcgccg acctccgccg cgttctccaa
    20221 gaccgcccgg cgaaagaggc gctcgaggcg atcgaggcct tccatcgcga ggcgggtgtt
    20281 tccggttacc gtttgcgggg cgaccagagg gagatccatc tgggtcgacg gaccgccttg
    20341 gcgccccgcg cgggacgtcc cggccgaccg tcaggggcgc accatccgat cgggccgaga
    20401 acgttcacca cgtctcaagg gtgggaggtc tgggtcggac gcaacaacac ccagaacgac
    20461 cagatcaccc accgtctatc gaacccccac gactactggc tgcacgtcgt gggggtaccg
    20521 ggttcccacg tgatcctgcg ccggccgtcg cggaacgcga tacccaagcc gagcacgctc
    20581 gaggaggccg cttccatcgc ggcctacttc agcaaggcgc ggaagctgac gcgggtcccc
    20641 gtgatctaca ccgaacgaaa attcgtgacc aggccacgcc gcggcaagcc gggacaggcg
    20701 ctgtgcacgc gggagcggga actgctcgtt cgcccgcggc tgcccgaggg cgatgccggc
    20761 acggagtcgg agtgaatcga ggtgtcgagc gagatgcgga gcaagaacgg gggggacccg
    20821 tttacggaaa ggaaccgggg tcgcggacag acggggcacc cagatagtgt catctagcta
    20881 ggagccttta tgtccatttg gactaggaga acgatgggtt tgccctggcc atggcaatct
    20941 cctgagagct ccccatcttc cgcgacccat acacactctt gtttggccgg atcatgttcc
    21001 gcactctggc tcgacccgac gcccatgcct aaaagcattc gccgtgcccc ccttcgtcaa
    21061 tcgacggtct ttcccaaatt catttgaaag tggttccaac catgtgagtt cggggtgtat
    21121 ccgcgtgccg ccggaggcgt cggtctggag gtccgcaaca ctccagtgag acatgaatgt
    21181 cacaccttga caggtatcga tccggataag tcgatggggt tcaacggggg tgtggcgatg
    21241 tcacggccgg agggggttta ggactggaat gtcacatgtc ccgcgcgccg cagcgcgcgg
    21301 aactcccagc gttgccgagg cacgggggag agacaggctt atgggccgag gaacagaggg
    21361 gggttcggag ctagtaaatc tgcttcaata accgaatcaa atgtctgcgc gtaatcccca
    21421 gctccagcgc cgcgcggctg cggttgccgc cgttccggga gagcgcggcc aggatctcct
    21481 cccgcttcag gaggtcgacc ttgctccgga gaccgcgagc ggcatgggtt tcggccgcgg
    21541 gcgagccgcc cggatgcagg agcttcacga gcatcccgcg atccaccagc tcctggcgtc
    21601 ccgcgcagag gaagatcgac tccaccttgt gcttcagctc gcggatgttt cccagccacg
    21661 gcagggagat caggtattcc agcgcgtcct cggagagcac cacctggcgc ccttcgcgca
    21721 ccgccagctc gtccaggaag gagcggatca ggagcgcgat gtcctcacgc cgctcgcgga
    21781 gcggcggcaa ggaaaggacg acctgcccca gccggtagta gaggtcggcc cggaaccagc
    21841 ccgccttgac cgcggcgtcc aggtccttgt tggtggccga gatgatgcgg acgttgagcc
    21901 gccgcgaccg cacctccccg agccgccggt aggtgccgtc gtccagtacc tgtaagagct
    21961 tcacctgcgt gcgctcggtc agctctccga tttcgttcag gaagatcgtc ccgccgtcgg
    22021 cttcctcgaa gatgccccgc ttggtgatgg cggcgccggt gaatgcgccg cgcacgtaac
    22081 cgaaaagctc gctctcgacg aggtcgcccg gaaggctggt gcagttcacc tcgacgaagg
    22141 gccggccgcg ctcgacttcg taggagcgga acatgtaggc gaagaggttc ttgcccgcgc
    22201 ccgtctcgcc ctgaatgagc acgcgggccg gcgtggagag gagctccgac gccttccgcg
    22261 agagctcgag catgcgcgcg ttctgggtca cgatgccgtg agcgcgggcg atctcctggg
    22321 cctgcgagga gccggtcccg gcctcgcggc gggcggcgcg cggcgtcgtg agccatgacg
    22381 gagcgttgag gtcgcgcagc agcgcctcgg cggcgcgaag cgaatcgtcg atgccgagct
    22441 gctcgaacag gtacatcgcc ttgaacgcga agttctgggc ctcgcgcatg cggtcggcgt
    22501 cgccgcgcgt cggcatgcgg cgggcctggt ccaggtaggc gcgcgccagc tcgaagcgct
    22561 cgtggatccc ttccaggatc cggatcgact ccttcgcttc ggcggcggct tcgggggcgc
    22621 tcccggcaag ggtctggatg gccgcgacca cgcgtcgggc ggctccctct tccagcaggt
    22681 ctttcagctt cgacgcgcgg tcccgcgcgt cctcggcggc ctcgagcgct tccggcagat
    22741 cgccgcgcag cgcgagcgcc tcggcgcggt ggcgctgcag ctcgtggatc atgtcccctt
    22801 ccggcgcgat ctcccgcgcc atcgcgaggg cctggtcgta gagcgcgagc gcctcgtcgg
    22861 tctcgcccat ctcctgcttc atctcgccgc ggctgaagag gcagaggatc tgctcgcgcg
    22921 aagcgccgag ccgcgacgtg atctgcgtcg ccgcctcgat gtgcgcggcg gcggcggcga
    22981 agtcgtgccg gaagatgtcg atggtggcga ggccgatccg cgtgcgcgcc acgccggact
    23041 cgcgtccgat ctgcacgtag gaggtgagcg cgcggtcgaa gtgcgcgcgg gcctcgtccc
    23101 aggagccggc cttcagatag acgatgccga ggttggcgca ggagacggcg atgtaggcga
    23161 agttgccgag gcgagtggcc agctccatgg agcggagcag gtgccagctc gcctggcgcc
    23221 agtccgagag gtggcggtac aggatgccca ggttgttgtg ggcgcggacc acgccctctt
    23281 cgtcgttcag gtcccagcgg aagaggtgga gcgcggcctc gtagtactcc ttggcctggt
    23341 ccaggttgcc cagccggaag tggatccggc cgaaacagtt gtacgagtgg gcgagcggcg
    23401 ctaagagccc ccgctccttg aagtaggtgt gcgcctgctc gcaggcccgg aacgcctcgt
    23461 cgtattcgga gcgctcgatc agggcgcggg cgctctgcag ggcgatgcgg gcggtcagct
    23521 cgggctgcga gggaccggtg acgcgcgcgc gggcccgcgc gagatagcgg agcgcggcgg
    23581 tggagtcgcc cttctcgatc agggtctgcg cggccttcat ctcgagggtg gcgatccgtg
    23641 cggcgtccag ccccagggct tcggcggagc ggatgcactc ttcgagattc tggagcgccg
    23701 ggaggtagct gtcagcgcga aggaagatct ccgccagatc ctcgagtgac tgaagatgcc
    23761 gctcggggtc gtgcgtgagc cgcgcgttcg aaaccgcctc ttcccgctcg cgaatcagct
    23821 cagccgtggt tcggtccacg tcctgcctct gcgttcgacc tgcgccggac tagaaccggg
    23881 tcgcgaaggt ccggaatacg ctgacgaaga tctgccaacg ctggagcgcg gtgagcctgg
    23941 tgtagacccg atgcgtgccg atcgtcgccg cgtcggtgga cgtcgttccg aagcccgtga
    24001 tgaatgcgct cttcggcggc gtcgaaggcg gcggggaatc cggcggcttc agcgtctcgt
    24061 caggcgagcc tccggcccac gcgatcatgg gtgtgagcgc gctccccgtg ttccgatcga
    24121 agcggagcgt cgggagcgag atcagcgtga gaagcgcgag cgtgaggacg gcgagtctga
    24181 gaacacgcgg taggaggagg cccatgtccg catcaccttc cgctaaggcc atcaagggag
    24241 gccgctccac cacgcgcgac cccgcacacc gtagggggtg aggaaccccc gtcacaggga
    24301 tataggatag cagaggaatt tcgatcgagg caacctgtgg caactatttt ttcagtttcg
    24361 gtcccggaat ccgctcatcc ccctcccgcc gggtgatccc cagccgatcc aggtgctcca
    24421 ggagcggaac ggcgtacttc cgcgagatct gcaggtagcc cttgaagtcc cccatgccca
    24481 gccgcgggct ccgggcgaag tgcgccgcga cgcgccgctc gatctcctcc cactgcgctc
    24541 gcgtataaac cagttccgaa gttaccttta ccgcgcggcc cgactcgatc atgtggcgca
    24601 tcagctcggt ggggcggctc tcccgaggca ccccccggag cacttcgctc agctccggcg
    24661 gctggaatcc ctgccccgcc agcttctgct cgatgcgctc gaggacctgg cggtcggctt
    24721 cgctaaggga gggcccggcg cccggcggaa ggagccggtc tccgcggacc tggaccgctc
    24781 ccgttgcgag aagggaggcc agggcctcgt cgaagacccc gccgtcgaga tcgcgggaga
    24841 ggaggctctt cagctccccc ttgggcactc cggcccggag cggatgcgcc tcggcgtact
    24901 cgcggacccg ggcctcgacc aggccgcggg cccgatccca cgcgtcggga aagagcgtcc
    24961 gtcccccctt cagccggacc agcgccttgc cgtccgcggc ggccgcggcg accgcctccg
    25021 ccccggcccc cagccggagc gcagcctcct cctcgcgcat gccctccatc cccgactccc
    25081 gggccagcca cagcaccttc tcgggaaggg tccccgactc cagcgtcttc agccgctcga
    25141 ggtcgccgcc ccccagcctg gaccggcgcg gcggagccgg gtcgatcacc acggccccgg
    25201 cgatggtcac cgccggcgaa taggagcgga gcaccagccg gtccccgggc gctgcgacga
    25261 cgggatgttc cagacggagc tgcgcgtagg cgctcgcgcc cggctcgagc ctctcccctt
    25321 cgagcaggac ggcgcgcccc agcacttcgc tcgcgcccag atggatccgg aggcgcgtgc
    25381 gcgtcgcgag cgcccgctcc ctcgggtcgc cgggaagcat cgtgagccgc acgtcgagcg
    25441 cctcggccgt gcggtagccg cccggcgtga ccagccattc cccgcgctcg atctcctcgc
    25501 gcccggcgcc gtgcagggcg accgcggtgc gctgccccgc cgccgcggcc acgacttcgg
    25561 cgtcgtggac ctcgacgcgc cgcacccgca cggaacggcc cgaggggagg gtctggagcg
    25621 aatcgcccgc ccggatcgtc ccgctccaga gcgtgccggt caccacggtg ccgatcccct
    25681 cgatcgagaa gatccggtcg atcggaaggc gcgcggggcg tccggcctcg cgcgcgccgg
    25741 cggaggcggc tgcctggtcg agcgcttcga ggagtgccgc gaccccttcg ccggtgacgg
    25801 cggagacggg gaggatcggc gatccctcga ggccggtgcc gcggaggagg gccgtgacct
    25861 catcgcgcgc gcgggcgagg cgttcgggcg gggcgaggtc cgacttggtg agcgcgacga
    25921 cgccgcggcg gatgccgagg aggtccacga tggcgaggtg ttcctgggtc tgcggcttga
    25981 ccccttcgtc ggcggccacc acgaggaggg ccacgtcgat cccggtcgcg ccggcgagca
    26041 tgttcttcac gaagcgctcg tggccgggga cgtccacgat ccccacccgc gcgcccgagg
    26101 gaaggacgag gtgcgcgaag ccgagatcga tcgagatgcc ccgctccttc tcctccttca
    26161 gccggtcggt gtcgatgccg gtgaggcggc ggacgagcgc ggtcttgccg tggtcgatgt
    26221 gccccgcggt gccgacgatg acggggcgca cgggacggag ccgggaggtg ccgggcgcgt
    26281 caggcccgga cgcggcggat gtccgccccg agccgcccga gcttctcctc gagccgctcg
    26341 tagccgcggt cgagatggta gacgcgcgag accttggtct cgccgcccgc caccaggccg
    26401 gccaggatca gcgccgcgga ggcacgcagg tcggtggcca tgaccggggc gcccgagagc
    26461 gactccacgc cgtgcaccag cgcgcggtta ccgtccacgc ggatgtcggc gcccaggcgc
    26521 tgcagctcgg ccacgtgcgt gaagcggtcg cggtagatgg tgtccaccag cgtgctcacg
    26581 ccgttcccca cggagaggag cgccatgaac tgcgcctgca tgtcggtcgg gaagccgggg
    26641 tacggcgccg tggtgaccga gagggcttcc ggacgctcgg gagcgcgcac gcgcgccgag
    26701 cggtcgcccc gctccacgga gcagccggcg gtctcgaact tggagaggac ggcgcccagg
    26761 tgcgacgggt cgatgttcgt gacctcgacg tcgccgcccg tgatcgcggc ggcggcgagc
    26821 agggtgccgg cctcgatccg gtcggggatc atcgcggtat
//
LOCUS       DASSLZ010000066         6894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1671798, whole genome shotgun sequence.
ACCESSION   DASSLZ010000066 DASSLZ010000000
VERSION     DASSLZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671798"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="VFM00_02330"
     CDS             complement(<1..1687)
                     /locus_tag="VFM00_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7497110.1"
                     /translation="MATLIRLGLSPLVGSNAPYSIYLLAVVVTAWVGGFWPGFAATFL
                     GYLAAEAFFVHPTGSTIDPFSTVGPAELIRFLSYALVGLSISFVSEQWHRVRLRDLTR
                     ARRLEQEVTERLRAAEDLRQSKQTLEALVTTAPIPIVVIEPDRTVRFWNLAAERIFGW
                     TAAEVVGRPIPIVPEEKCEECETSRLAVSRGETVGPIATYRRRKDGTLVDVELSAAPL
                     YNADSLVDSMVLLLNDVTEQRRAERSLRESQAMLREADRRKDAFLATLAHELRNPLAP
                     IRNGLQVMRLAGSDAGAVERSREMIERQLGQMVRLIEDLLDVSRITRGTIELRKHRMH
                     LQDAILSAVETSRPVIDSLGHELMVSIPEEPIALDADLTRLSQAFANILHNAAKYTDK
                     GGRIALHADRQGACAVVSVSDTGIGIHPEMLPTIFGLFMQGDPTQKNARGGLGVGLSL
                     VKQLVEGHGGTIEARSDGPGRGSEFVITLPLLEAPSVAGAASDGNAAAALPSRPANRR
                     RVLVVDDNADSVESLATLVSMMGHECAKALDGERAIATARSFRPDTILLDLGMP"
     gene            complement(1988..2626)
                     /locus_tag="VFM00_02335"
     CDS             complement(1988..2626)
                     /locus_tag="VFM00_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497111.1"
                     /translation="MKTLALHIAIAAMLLPPAVSTAGTTPMTPETTRAHRTASSYDST
                     PATSLVSAGAVEFSPTVTFTHQNFKREGYGNVDHFTQLDVKPTVGFCLSDHYEVNAGF
                     LARHTSTNGSDDTALGATAGLTYNFSRHGSVIPFASLGFGALFYDGFTMDNTAVLAPM
                     LSGGVRVPVGAMASVNMSVGYQHESNADGEFNASANRILASVGVSLFPWRTK"
     gene            2944..4134
                     /locus_tag="VFM00_02340"
     CDS             2944..4134
                     /locus_tag="VFM00_02340"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HET7497112.1"
                     /translation="MALAAAGVLAFTGCGKLLTTKPAAGERFDQPLDGLSNGELGDFQ
                     DGHTQFRKAFTINEGLGPIFNNVSCASCHSGDGRGKPENVLTRFSRGTDLDFSEGGPQ
                     LQDKAIPGAVAERLPSGVDVSYRLPPPVFGVGLIEAISDSTIAAGEDPGDANGDGISG
                     RVNWVTPAPYLPADDPGGGTTPRAGRFGRKGSVSSLVQQVTEAYHQDMGITSEFRPTE
                     NTNPLASAIAAGVDRAPDPEVSVAKIHDIAQYMRMLAPPSPGEMTPARERGQTLFAGA
                     RCTGCHMPVLHTGPHEIEALANRDAALYSDLLLHDLGDALADNRPDGSADGHEWRTAP
                     LWGMRIAREFLGGGLLLLHDGRAHSVDEAVRLHGGEASAARDAYIAMPTGDKADLVDF
                     VESR"
     gene            4320..5762
                     /locus_tag="VFM00_02345"
     CDS             4320..5762
                     /locus_tag="VFM00_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497113.1"
                     /translation="MAVWALVSWATASAGTTPAAPKKTSDTEQTTTEAARAAAASAAT
                     VARTTPPVTTVAAHTVSTSATTAAGPATAPASATTAASRTTADATHPAPTRVSPPRTG
                     GTGHGTTTRSDPATVLVVQDEHASSPDAAPYHGSLENIAWGGHGELRFGYDLTAGVSD
                     PSWFHVGRANGFFLAKLGSRLRLGGQASYDRVPDDFTLERAELIARLGRGAREAHAGI
                     FLVPLGRTNLDHDAPQYEFGERSLVATEILGVPNAELGAGLRGAGSWSRTMPFTYEVD
                     LVTGFDDGIVMDAAGGTRLPAGRNNYGDQNGLPALSARFAIQPSVDTEIGLSAQSGPY
                     NQTKVDGVKVDRTRFVHVVAADAVTNAVGVDWAAEAAGALVDIPPSLEALYAQRQWGA
                     SLEATRVLRAPLFAAWHGTSLSGGLRAEKVDFDMGIPGDSRARVGASLNIRPLPSAIV
                     RFGWYYEWRRDRFDNLTPLGGLTLTAASYF"
     gene            6001..6858
                     /locus_tag="VFM00_02350"
     CDS             6001..6858
                     /locus_tag="VFM00_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497114.1"
                     /translation="MRDYRLIHISDDILLHGLANLVASDRQTTADLLAHLAEFDARRL
                     YVPAGHASMFAYCVEALHFSEDAAYKRIQAARAARRFPSLFEAIADGRLNLTAVGLLA
                     PHLTAENAQELIEEATHKLKAEIQEALARRFDSRAVRPNGPASTIRPIPQLAPAQVRT
                     ALQEGQLALAQVEISSGLNGATPGLNGATPGLIGATPGLNGAVPGLNGAGSGSSSNHP
                     DPAPVVPGLGVGTPGLQQPVPAQASASWSSSRSTRPPTTSCAARKLFSATPFLPEMWS
                     RCSTAPWML"
BASE COUNT         1094 a         2315 c         2370 g         1115 t
ORIGIN      
        1 cgggcatgcc cagatcgagc aggatcgtgt ccgggcggaa ggaccgggcg gtcgcgatgg
       61 cccgctcgcc gtccagcgcc ttggcgcatt cgtggcccat catcgagacc agggtggcca
      121 ggctctccac cgagtcggcg ttgtcgtcca ccaccaggac gcgccggcgg tttgccgggc
      181 gcgatggaag ggcggccgcg gcgttgccgt cgctggccgc gccggccacg ctgggcgctt
      241 ccagcagggg gagcgtgatc acgaactcgc tcccgcgtcc cgggccgtcg ctccgcgcct
      301 cgatcgtgcc gccgtggcct tccaccagct gcttcaccag gctcagcccc acgcccaagc
      361 ccccgcgagc gttcttctgc gtggggtcgc cctgcatgaa gaggccgaaa atcgtcggaa
      421 gcatctcggg atggatgccg atgccggtgt cgctcacgct gacgaccgca caggctccct
      481 gccgatcggc gtgcagcgcg atccggccgc ccttgtcggt gtacttggcc gcgttgtgca
      541 ggatgttcgc gaacgcctgc gagaggcggg tgaggtcggc gtccagcgcg atcggctctt
      601 ccgggatgct caccattagc tcatggccga gggaatcgat caccggccgg ctggtctcca
      661 ccgcgctcag gatggcgtcc tgaaggtgca tgcggtgctt ccgcagctcg atcgtcccgc
      721 gcgtgatgcg gctgacgtcc aggaggtctt cgatcaggcg caccatctgc cccagctgcc
      781 gctcgatcat ctcgcgcgag cgttcgaccg cgcccgcgtc gctgccggcg aggcgcatga
      841 cttgaaggcc gttccggatc ggcgcgaggg gattccgcag ctcgtgggcc agagtggcta
      901 agaaggcgtc cttccggcgg tcggcctcgc gcagcatcgc ctggctctcg cgcagcgagc
      961 gctccgcgcg gcgctgctcg gtgacgtcgt tcaggagcag caccatgctg tcgacgaggc
     1021 tgtccgcgtt gtagaggggc gccgccgaca gctcgacgtc caccagcgtc ccgtccttgc
     1081 gcctgcggta ggtcgcgatc gggcccaccg tctcgccgcg gctcacggcc aggcgcgagg
     1141 tctcgcattc ctcgcacttc tcctcgggga cgatcgggat cgggcgcccc acgacctcgg
     1201 cggcggtcca tccgaagatc cgttcggcgg cgagattcca gaagcgcacg gtgcgatcgg
     1261 gctcgatcac cacgatcggg atcggcgccg tggtgacgag cgcttcgagc gtctgcttgg
     1321 actggcgcag gtcttccgcg gcgcggagcc gctcggtgac ttcctgctcg aggcgccgtg
     1381 cgcgggtgag gtcgcggagc cgcacgcgat gccactgctc gctgacgaag ctgatggaga
     1441 gcccgacgag ggcgtaggag aggaatcgaa tcaactcggc cgggccgacg gtggagaagg
     1501 ggtcgatcgt agatcccgtg ggatgaacga agaacgcctc ggcggcgaga tacccgagga
     1561 acgtggccgc gaagcccggc cagaatcctc cgacccacgc ggtgacgacc accgcgagaa
     1621 gatagatgga gtagggagcg ttcgagccga ccagcggcga aaggccaagg cggatcagcg
     1681 tcgccacggc ggtggcgacg agcgcgactg cgagtccaac gcttaaattg cggtggggga
     1741 gatgcatgcg gccgcctgcc tcggtctcgt gcaactgtga atggtaattg caatgatttt
     1801 acgcagttgg aaggttcagg caagaggtaa cttagaattg aggcagtaag gctaaattat
     1861 tggggggggc caatttgtgt cgcagtgcgt ttcgcgccag gaggacgcgc aataccgagc
     1921 ggtactgaac gtactcctac tcgcgtacta ctacgcgccg tgctcctgct cgcgtgttcc
     1981 tgggttccta cttcgttctc cacgggaaga gcgacacgcc caccgacgcc aggattcgat
     2041 tcgcgctggc gttgaactcg ccgtcggcgt tcgactcgtg ctggtagccc acgctcatgt
     2101 tgaccgaggc catcgcgccc accggcacgc gcaccccgcc cgagagcatc ggggcgagca
     2161 ccgcggtgtt gtccatcgtg aagccgtcgt agaagagcgc gccgaagcct aggctggcga
     2221 aggggatcac cgagccgtgg cgcgagaagt tgtacgtgag gcccgccgtc gcgccgagtg
     2281 ccgtgtcatc gctcccgttc gtcgaggtgt ggcgcgcgag gaaccccgcg ttcacttcgt
     2341 agtgatcgct caggcaaaag ccgaccgtgg gttttacgtc gagctgcgtg aagtggtcca
     2401 cgttgccgta gccctcgcgc ttgaagttct gatgcgtgaa ggtgaccgtg gggctgaact
     2461 ccaccgcgcc ggccgagacg agcgacgtgg cgggggtgct gtcgtagctc gaggcggtgc
     2521 ggtgggcgcg ggtggtctcc ggggtcatgg gcgtggtgcc ggcggtggag acggccggcg
     2581 ggagcagcat ggccgcgatg gcgatgtgca gtgcgagcgt cttcatcggt tccccccggg
     2641 ttttcctggg ttcccttgga gtggcggccc cgcgtttcat tgatccttct cgcagcggat
     2701 catctccctg aacgctgcgg ttcactagct gactgactga gtcagtctga ctgactgagt
     2761 cagtcaaata ggaccagacc ggccaaggca aattccggac ccacgacctc cgcgagaccg
     2821 ccaaccctgg cctgcctctt gccaactacg acttggaccc gctccaaacc tcgggggagg
     2881 cgagccattc ccatgtccgt catgcggcat tcgcatgctc gaattgcgtt ttgcgcggct
     2941 ggggtggccc tggctgccgc cggcgtgctc gcgtttacgg gctgcgggaa gctgctcacg
     3001 accaagcccg ccgcgggcga acgcttcgat cagccgctgg acgggctctc gaatggcgag
     3061 ctgggcgact tccaggacgg acacacccag ttccggaagg cgttcacgat caacgagggg
     3121 ctcggcccca tcttcaacaa cgtctcgtgc gcgtcctgtc acagcggtga cgggcgaggg
     3181 aagcccgaga acgtcctgac gagattcagc cgagggaccg acctcgattt cagcgaggga
     3241 gggccgcagc tccaggacaa ggcgatcccc ggagccgtgg cggagcggct tccgtcagga
     3301 gtggatgttt cctaccgcct cccgccgccc gtgttcggcg tggggctgat cgaggcgatc
     3361 tccgattcca cgatcgcggc cggggaagac cccggcgacg cgaacgggga cggcatctcg
     3421 gggcgcgtga actgggtcac tccggcccct tatctgcccg ctgacgatcc cggtggcgga
     3481 acgacgccgc gcgcgggccg ctttgggcgg aaggggtcgg tctcttccct ggtgcagcag
     3541 gtgaccgagg cctatcacca ggacatgggc atcacgtcgg agttccgccc gacggagaac
     3601 acgaatcccc tggcttccgc catcgcggcg ggcgtggatc gagcgcccga tcccgaggtg
     3661 agcgtggcga agatccacga catcgcacag tacatgcgca tgctcgcgcc cccgtcgccg
     3721 ggagagatga ctccggcgcg ggagcggggg cagacgctgt tcgcgggcgc acggtgcacc
     3781 ggctgccaca tgcccgtgct tcacaccggc ccgcacgaga tcgaggcatt ggcgaaccgg
     3841 gacgcggcgt tgtactccga tctcctgctg cacgacctgg gagacgcgct cgcggacaac
     3901 aggcccgacg gcagcgccga cggccacgag tggcgcaccg cgccgctctg gggcatgcgc
     3961 atcgcgcggg aattcctggg cggcgggctg ctcctgctgc acgacggccg cgcgcacagc
     4021 gtggacgagg cggtgcgcct gcatggcgga gaagcctccg ccgcgcgcga cgcctatatc
     4081 gcgatgccga ccggcgacaa ggcggacctc gtggacttcg tggagtcgcg atgagaatga
     4141 cgaatcgaga tcaggcggcg cttatggcgc tgcctatgaa gagccggctc gcgtccgaga
     4201 atggggcgag tccgcaaggg gcggcgctca cgagaagggc gtcgccgagc gaggcgcctc
     4261 ccacgagagg ggcggcgcca catggggcgg cgcggaagcg agtggtcgga gccggtctga
     4321 tggcggtgtg ggctctcgtg tcttgggcca ccgcttcagc cgggaccact cccgccgctc
     4381 ccaagaaaac gtcggacacg gagcagacca cgacggaggc cgcccgcgcg gcggccgcga
     4441 gcgcggcgac cgtcgcgcgc acgacgccgc cagtcacgac cgtcgccgcg catacggtat
     4501 ccacgagcgc gacgacggcc gctgggcctg cgacggcacc cgctagtgcg acgaccgcag
     4561 cctcccgcac cacggccgac gccacgcatc ccgcgcccac gcgcgtatcg ccgcctcgaa
     4621 ccggcggcac cgggcacggc acgaccacgc gctccgatcc cgcgacggtt ctcgtcgtcc
     4681 aggacgagca tgcttcctcg cccgacgccg cgccctacca cggcagcctc gagaacatcg
     4741 cttggggcgg gcacggcgag ctgcgctttg gctatgacct caccgccggc gtttccgatc
     4801 cctcctggtt ccacgtgggc cgcgcgaacg gcttcttcct ggcgaagctg ggatcgcggc
     4861 tgcgcctggg cgggcaggcc tcatacgacc gggttcccga cgacttcact ttggagcgcg
     4921 ccgagctgat cgcgcgcctc ggccgtggag cgcgcgaagc gcacgcgggg atcttcctcg
     4981 tgccgctcgg ccgcacgaac ctggaccacg acgcgccgca gtacgaattc ggcgagcgct
     5041 cgctggtcgc caccgagatc ctgggcgtgc ccaacgccga gctgggcgcg gggctgcgcg
     5101 gcgcgggatc gtggtcgcgc acgatgccct tcacctatga agtggacctg gtcaccggct
     5161 tcgacgacgg catcgtgatg gacgccgccg gcggcacgcg acttcccgcc ggacggaaca
     5221 actacggcga ccagaacggg ctgcccgcgc tctcggcgcg cttcgccatc cagccctcgg
     5281 tggacacgga gatcggcctc tccgcgcaga gcggacccta caaccagacg aaggtcgacg
     5341 gcgtgaaggt ggaccgcacg cgctttgtcc acgtggtcgc ggccgacgcg gtcaccaacg
     5401 cggtgggcgt ggactgggcc gcggaggcgg ccggcgctct cgtggacatc ccgccctcgc
     5461 tggaggcgct gtacgcccag cgtcagtggg gcgcttcgtt ggaagcgacc cgtgtgctcc
     5521 gcgcgccgct gttcgcggca tggcacggca cgtcgctgag cggcgggctc cgcgcggaaa
     5581 aggtcgactt cgacatgggc atccccggcg actcacgcgc gcgcgtgggc gccagcctga
     5641 acatccggcc tcttccctcg gcgatcgttc gttttggttg gtattacgaa tggcgtcgcg
     5701 atcgtttcga taatctgact ccgcttgggg gtttgacgct tacggctgcc agttacttct
     5761 aggggaggcg ccgccgctcc accgctccgc gctccgcgta catcgcttcg cgcaccgacc
     5821 cactccgctg catttcgtca tcacattcct gatccacgag cacggttcat gcctcgaaag
     5881 tcttcatggc acaacgcgaa gcgaatcccc gttgttacgt cgagcgttgc gcgcgcacgc
     5941 gtcaacgcgc ggctacgtcg acgtcgcctc caggaaaccg ccccgtaaac aggtgtttgc
     6001 atgcgcgact atcggttgat ccacatcagt gatgacattc tgctccatgg gctcgcgaat
     6061 ctcgtcgcga gcgatcgcca gaccaccgcg gaccttctgg cgcatcttgc ggagttcgac
     6121 gcgcgccggc tgtacgtccc ggccgggcac gcgtcgatgt tcgcgtactg cgtcgaagcg
     6181 ctgcacttct cggaagacgc ggcctacaaa cggatccagg cggcacgagc agcgcggcgg
     6241 tttccatcgt tgttcgaagc cattgccgat ggccggctca atctcaccgc ggttggcctg
     6301 ctcgccccgc acctcacagc ggagaacgcc caggagctga tcgaggaggc gacacacaag
     6361 ctgaaggcgg agatccagga agcgctcgct cgccggttcg actcgcgggc ggtgcgaccc
     6421 aacgggcccg cgtccacgat tcgccccatc ccccaacttg cgccagcgca agttcggacg
     6481 gctctgcaag agggacaact tgcgctggcg caagtcgaga tcagctccgg attgaatgga
     6541 gccacgcccg gactgaatgg agccacgccc ggattgatcg gagccacgcc cgggttgaat
     6601 ggagccgtgc ccgggttgaa tggagccggg tcagggtctt cgagcaatca ccctgatccg
     6661 gctcccgttg tgcctggctt gggcgttgga acgccgggcc tccagcagcc agtccctgcc
     6721 caagccagcg cttcctggtc aagctcacga tcgacaaggc cacccacgac aagctgcgcc
     6781 gcgcgcaagc tcttctcagc cacgccattc ctgccggaga tgtggtccag gtgctcgacc
     6841 gcgccctgga tgctctgatc acgcagctgg agcggcggaa ggccggggcc gagc
//
LOCUS       DASSLZ010000067        22627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_25560, whole genome shotgun sequence.
ACCESSION   DASSLZ010000067 DASSLZ010000000
VERSION     DASSLZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 22627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22627
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25560"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VFM00_02355"
     CDS             <1..1063
                     /locus_tag="VFM00_02355"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497115.1"
                     /translation="FRKDLEIEEDLIEEVARGIGYDAVPATVAAASVPAIAESPERAF
                     AARVVDVAVGLGFDEAIGAALVGDIPAEAREGIEAASIWEIQNPKSRELKHLRVSLLP
                     GLLAIAARNARHGVTDVRLAEVGKVFQAQPPPLGSERLEAAFLLTGSPDPWNRPGADS
                     DRYLELRGAVEALYEALGIDSWQGGSYHDACWARGTGSVWAVSGSRLGRMGEVAPSLA
                     RQMGLTQPAWAAIVDLAAALRAAPRERRYREVPRFPASKRDLAVVVRPDVTHEDLAAT
                     IRAAGGPLLHTIRLFDVYKFRDGEHAGRSSLAYALEFRSPERTLTDREVEDSFAAIVR
                     ALESKLGAALRGGAEVPRA"
     gene            1060..1359
                     /locus_tag="VFM00_02360"
     CDS             1060..1359
                     /locus_tag="VFM00_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497116.1"
                     /translation="MSAIVTELSALEERVQQIVSLLERMREENRALTGERDDLRAKVR
                     ALEAAAGKHAAADYKTRLKSLEDERATLLEERRALARRVEEMLAKLSVLEKAVHA"
     gene            1428..1721
                     /locus_tag="VFM00_02365"
     CDS             1428..1721
                     /locus_tag="VFM00_02365"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET7497117.1"
                     /translation="MGSEPTTQSVTIFGSEYKIKGADPDYIQTVAAYVDGKMRELERR
                     LATGTPTKIAILASLNIADELLRERAERGRLDKELAERTKALTKTIGDSLRER"
     gene            1786..1966
                     /gene="ssrS"
                     /locus_tag="VFM00_02370"
     ncRNA           1786..1966
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFM00_02370"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1991..3559
                     /gene="rny"
                     /locus_tag="VFM00_02375"
     CDS             1991..3559
                     /gene="rny"
                     /locus_tag="VFM00_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET7497118.1"
                     /translation="MTVSLLTLILAALGSGIVAFLLGWIAHRKIGEGKVLRAEELAAK
                     ILREAEREAETQKKSALLEAKDEWFKEKSRLDREAQAQKNEIQRAERRLQELETNLNR
                     RAEVLDKKERDQKRLERDLSVKGEALELRDKELERVLGEQNARLQRISGMTAEEAKQL
                     LIVNMENEARAEAGKLLQQIREESTRNAEKEAREIVTLAIQRCAADHSAETTVSVVHL
                     PSDDMKGRIIGREGRNIRAFETVTGIDVIIDDTPEAVILSGFDPVRREIAKRSLERLI
                     TDGRIHPARIEEVVAKVQKELEEQIIELGEAAVLEVGVHGMHPEIVKLLGRLQYRTSY
                     GQNILQHSKEVAWICGIMAGQLGFDTQIAKRAGLLHDLGKAVTHEVEGAHAQISMDFA
                     QRYGEPQEVVRAVGYHHHDPTAENLYAVLVMAADAVSGARPGARRESIENYVKRLEAL
                     EKIADSFPGVEKSYAIQAGREVRIMVEPKNVDDARASQLASDVARRVERELQYPGQIK
                     VVVIRETRAMEYAR"
     gene            3567..4373
                     /locus_tag="VFM00_02380"
     CDS             3567..4373
                     /locus_tag="VFM00_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HET7497119.1"
                     /translation="MNILFVADIFGSPGRRAVRDLLPEIVSSMGIDLVVVNVENAAAG
                     FGVTKEILEEVKGLGVQCMTSGNHIWDRREVMPLLDSEPLLLRPHNYPPGVPGRGVMI
                     ARTASGAKVGVINLMGRVFMRELLDPFRVAEEEAAKLREETPIILVDFHAEATAEKVA
                     LGWHLDGKVSAVIGTHTHVQTADERILPGGTGYITDAGMTGPHDSVIGVRKDQAIRKF
                     LTLLPTRFEPATGDVRLHGVHLEVDDATGRCRHIERVAMALEPAPGAAVP"
     gene            4370..5278
                     /locus_tag="VFM00_02385"
     CDS             4370..5278
                     /locus_tag="VFM00_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HET7497120.1"
                     /translation="MTTAPAAALLLGGPLAAEIRAGVKRRLEALRDRGRPAGLAVVTV
                     GEPPSGNPYVRAKVKAAQEAGAVATVHRLSEDIAEPKLRDALRAIGDDPAVHGLILQL
                     PLPRHLREERHLEDVPPAKDVDGIHPANVGRWVNGLPAPRPATPLGVQELLRRHCGDL
                     AGKRAVILGRSRIVGRPLSVLLSSREPGMNATVTLCHSETRDLPALCREAEILVVAIG
                     HRRSIGAAHVRPGAVVIDVGIHPIANPAPGAPRYEGDVDFEAVRGIASAITPVPGGVG
                     PLTVAMLLRNLADAAEAAEAAEGGGS"
     gene            5508..6995
                     /gene="xseA"
                     /locus_tag="VFM00_02390"
     CDS             5508..6995
                     /gene="xseA"
                     /locus_tag="VFM00_02390"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET7497121.1"
                     /translation="MAGQRLAPKDPDGHTQTLAAKDRDGRTMSARRNWGAAARFDPRE
                     GEPHVYTVGEIVAELNALLGGEYPSITVEGEITDFRAHPSGHEYFCIKDRRAQLQVVF
                     FASQPRIRGVQLKNGLAVRVTGTITVYEARGSMQLKAERVSPVGFGALQIRFEELKRK
                     LQAEGLFAVERKRELPRYATRVAIVTSFSGAAIRDMLRILRQRAPHARITVAPARVQG
                     DGAALSVAAAIRLVNDWGDADVLIVGRGGGSLEDLWAFNEEAVVRAIVASRIPVISAV
                     GHESDITLADLAADVRAATPTHAAQQAAPSREEARHKLHQLAVHARERLLREMQRDRR
                     HLDGYRTHRAFREPHRQVAEGRQTLDNTRDVLARALEGWALRRRSTVGSASTRLRAHA
                     PARVVERMRERLLELRRRAERASITDLAKRRKEAGARARLLTSYDYRGVLRRGYALVW
                     NEEGARLVNRGRNLRPGEGIRVQFHDARAEASVTRVDPSTEEETP"
     gene            6992..7279
                     /gene="xseB"
                     /locus_tag="VFM00_02395"
     CDS             6992..7279
                     /gene="xseB"
                     /locus_tag="VFM00_02395"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET7497122.1"
                     /translation="MKRKEGPAPDAPTFESMMERLEQLVGTLESGNLSLEESIHSFEE
                     GMALVKRCTEVLDQAEQRIQKLTRDAQGAPRTEPVAEDDEADEGRSGELPF"
     gene            7248..8150
                     /locus_tag="VFM00_02400"
     CDS             7248..8150
                     /locus_tag="VFM00_02400"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET7497123.1"
                     /translation="MKGGAASSRFERYLERARPRIDRALDGVLPRASAEPRRLHAAIR
                     YSVFAGGKRLRPALCLLACESVSGSCSDAMHAAAALEMIHTFSLIHDDLPGMDDDDWR
                     RGRLTNHKVFGEGMAILAGDALLALAFETLATRPTGRAKDPRALLRTVCEATGHEGMI
                     GGQALDLLYERQRVSFARVLRMHRRKTGMLLRGSLLAGAQVAGASAAALRRFGDYGDR
                     IGVAFQITDDILNERSTKRATGKSVRSDRARGKATAPSSGGMARAEREVERLLDEAAA
                     IAPRLGRRAEEFASLTEFLRHRTR"
     gene            8186..8632
                     /locus_tag="VFM00_02405"
     CDS             8186..8632
                     /locus_tag="VFM00_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HET7497124.1"
                     /translation="MGFTANPLLHALLCGFLVQLSKVLTFLLQERKINVRRFVETGGM
                     PSSHAASVMALTTCVGLREGVRSVLFGVVLYFSLIVMYDAAGLRRAAGRQATLLNRIL
                     HEHLQLSGPPAERLRELLGHTPIEVLVGALIGVLFSFAIYRPPGGP"
     gene            8629..10599
                     /gene="dxs"
                     /locus_tag="VFM00_02410"
     CDS             8629..10599
                     /gene="dxs"
                     /locus_tag="VFM00_02410"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET7497125.1"
                     /translation="MSTASASMNASTNHLAGISSPEDLKRLPPEALPALAAEIRERIL
                     SVVSKTGGHLAPSLGTVELTIALHYVFHTPEDILIWDVGHQAYGHKILTGRNDRFDTI
                     RREGGLSGFPRRVESPYDPFGTAHASTAISAALGFACARDLAHEKRRVVAVVGDGALT
                     GGLAYEGMNQAGAAGTDLLVVLNDNSMSISPNVGAISQYLTRLTAGPIFRRLEADLWD
                     LLGKLPAGGTARKLAGRIKESMKNLVVPNVLFEELGFKYFGPIDGHDLTTLLTVLNQV
                     KEVKGPVLLHTLTRKGKGYGPAEEDSRKFHGVSSFDKLTGASARKTSGPAYTEIYGQS
                     MIELARQDPKIMAITAAMTDGTGLTKFATTFPDRFHDVGIAEQHAVCFAAGVAAAGLR
                     PFATIYSTFLQRAYDQIVHDVAVQGLPVRFALDRAGLVGEDGSTHHGVFDIGYLRCLP
                     GMVLMAPKDENELRRMLVTMAAHDAGPIAVRYPRGAGVGATLDPNPQPLAIGEAELIR
                     SGKDVVLVAYGAMVAVAEKTASLLAESGMDAAVINARFAKPLDEAMLLDWARRVPRLV
                     TLEEGALPGGFGDAVLELFAKTTVPGVQIRTFGVPDRFFDHGSRESLLLAAGLHPDLL
                     APEIRRFLAGDPRSTPEPLAPKASSAPIATHA"
     gene            10596..11492
                     /locus_tag="VFM00_02415"
     CDS             10596..11492
                     /locus_tag="VFM00_02415"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET7497126.1"
                     /translation="MIRPPRHLGIVGNRGKEGVRALVPPLIRWARAQGRKVSLDRELA
                     RGMRGVGAGITLEALVKRADAILVLGGDGTMLGTARAASAAGVPILGVNLGGLGFLTE
                     TAQEDLYPALERLFRGDVTLERRTMVEAQVRRRGSKGVWQEVGLNDAVIHPSERSRVI
                     AMDLRIAGKEIGTLVGDGLIVASPTGSTAYSLSAGGPIVRPSVEALLATPISPHTVTW
                     RPLLVGADETIRVRLRPGHPRANLTMDGQVARTVTPDDEILIRRARRRATLIVLEPES
                     FYDVLRTKLAWATSPRGRVPDQ"
     gene            11508..13286
                     /gene="recN"
                     /locus_tag="VFM00_02420"
     CDS             11508..13286
                     /gene="recN"
                     /locus_tag="VFM00_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET7497127.1"
                     /translation="MLRWLSIQGLALVDSVELEFAPGLNVLTGETGAGKSLVLGSIGL
                     LLGDRADSAWLRSGESRGSVEGTFDLAGRPDLIEALRAHDVEPEEGRVVLRREVAANG
                     TTRAFVNGRGVLLVQLRAVGDLLVDLHGQHEHQQLLDPARQADFFDAWAGTWEERRAL
                     EAERAAILEDRRAAAAARAALERDRAEEETLRADLAELEALKLEDGEEETLLRDRERL
                     VHKERILQGLSEALALLADEETGAESRLRRAVKALRTGAALDAGLEPLVEEAEAASEA
                     ASALSSRAEGERARLLEEPLDLDRVEERLDRIHRLKRKHRTDASGLLALAGELQTRVR
                     QLAPGAEEVDRLERALAARTAAYGRRLDALSERRATRAAAFERAAGALLDRLGFGKAR
                     LGAAPSGARAGDRSRPAIDPAPIPALEFEFQPNPGEPPRPLRRIASGGELSRVMLAVK
                     SLMADKDQVAVLVFDEVDQGIGGAVGEEVGALLRDLGDKRQVLCITHLPLIAAYAQRQ
                     LQVAKSVARGRTTTTVAPLSEEERVEEIARLLAGARAGETTRRQARELLSGARSGAGA
                     AAGPSRKCASAPQGAVSRARTAKGKR"
     gene            13283..14758
                     /gene="trpE"
                     /locus_tag="VFM00_02425"
     CDS             13283..14758
                     /gene="trpE"
                     /locus_tag="VFM00_02425"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET7497128.1"
                     /translation="MITPSLEDYRALARRYNLVPVMREVLADFDTPVSAFAKLNRGDA
                     SFLLESLEGGETWGRYSILGFRPSIEFRAKGAVVETRRGERTERTESQDPLESLRALL
                     QSVSAAPVANLPPFSGGAVGLVGYDYVRFLERIPARVPDDLGQPDLHFVFPDLVLAFD
                     NFRHRLQLVANTRPGNDPDRAYRDAVAAISEMLSRLAQPAPAPAATERANGEVAFTSN
                     LGREGYAQAVLAAKEHIRAGDIVQVVLAHRLEATAAVSPFDVYRALRILNPSPYMYYL
                     RYSDRSVAGSSPEILVRTSGRKIALRPIAGTRPRGQSREEDAALEQELLTSEKDRAEH
                     VMLVDLGRNDVGKVAEIGSVRTTEFMTVERYSHVMHLVSHIEGTLRDGLGPFDVLRAC
                     FPAGTVSGAPKKRAMEIIESLEPSRRGAYAGAMGYFDFHGNADFCITIRTATFEGGRV
                     HLGVGAGIVADSDPEEEWTETRNKGRAVEAAVRLAAGGLDA"
     gene            14755..15366
                     /locus_tag="VFM00_02430"
     CDS             14755..15366
                     /locus_tag="VFM00_02430"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET7497129.1"
                     /translation="MILMIDNYDSFTWNLVQYLGELRETPRVVRNDEITVDEALAMKP
                     DAIVISPGPGRPDDAGISKELIRRSAGVVPILGVCLGHQCIGEVFGGTIVRAERLVHG
                     KTSAIIHTGRGIFLDVDNPFMATRYHSLLVAREGLPDALAVVAWTPENEIMALRHKDH
                     ETWGVQFHPESILTAHGKDLLRNFLRLVPDAAAARPAAAAPAR"
     gene            15458..16495
                     /gene="trpD"
                     /locus_tag="VFM00_02435"
     CDS             15458..16495
                     /gene="trpD"
                     /locus_tag="VFM00_02435"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET7497130.1"
                     /translation="MIRDLLPRLLAGETLSRAEAAAAVRSMVRGESEDGLVASFLTLL
                     AQRGETEAELWGGAEALRAEAVPFPWNGGALFDTCGTGGDGRGSFNVSTTAAFVVAGA
                     GVRVAKHGNRSVSSACGSADVLESLGAHIDLGPEGAAAVLAETGFTFLYARRFHPAMR
                     RVAGVRQTLGFRTLFNWLGPLSNPARATHQLVGVPDAARVEPVARVLAGLGAVRALVV
                     HGAGGLDELALAPGNLAAESARSETGAPAALPRSIRHDALGLRDAPLEALRGGDADRN
                     AALLREVLAGEPGPRRDAVLLNAAAALWIAGAAPTLDDALARATQAIDRGDARKVLDH
                     YIELSKKVGSE"
     gene            16492..17307
                     /locus_tag="VFM00_02440"
     CDS             16492..17307
                     /locus_tag="VFM00_02440"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010142512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET7497131.1"
                     /translation="MSDFLATIVAERLDRVRQDAERGPSRAALRAEAEARRDERRPFL
                     SALRREAGAPIRVIAEVKRASPSAGVLQAEYDPASLARAYDEAGAAAISVLTEPARFL
                     GSLEDLAAVRAAVGLPVLLKDFVVHERQIYEAGARGADAALLIVAALDPRQLGDYAAL
                     LVELGIAPLIEIHEAREIDAALAAPGALGVNNRDLRTLTMRPGHAEGMLPLLPPDRVR
                     IAESGYHDRAAVQRVERLGADAVLVGEALLRAERPSQAFAALFGTGPSSEEES"
     gene            17304..17936
                     /locus_tag="VFM00_02445"
     CDS             17304..17936
                     /locus_tag="VFM00_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET7497132.1"
                     /translation="MRTRIKVCGITSAEDARQALDAGADFVGVVLTESPRRVDAARAR
                     AIRESLPAGTPLVGVFAEEPPETVAPLAVELGLHAVQVAGWLEREERFPFEVWHVLRG
                     TTLPDPTTLPMIPLRTYHLDAYDPARPGGTGRLADWAWAASAVEVGRRLIVAGGLTPE
                     NVATLVRDVRPYGVDASSGLESAVGKKSPEKVRAFVERIRAADQSRPKRS"
     gene            17933..19153
                     /gene="trpB"
                     /locus_tag="VFM00_02450"
     CDS             17933..19153
                     /gene="trpB"
                     /locus_tag="VFM00_02450"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7497133.1"
                     /translation="MSARANPQGEGRFGGYGGRYVPETLLGPVLELAEAYEAARQDPA
                     FQAELESELRSFAGRPTPLALAPRFAARAGLTRVYLKREDLLHTGAHKINNAIGQALL
                     ARRMGKKRLVAETGAGQHGVASATAAARYGLECTVYMGEVDMARQRPNVERMWLLGAE
                     VRPVASGSRTLKDAINEAMRDWSGSVRDTHYLLGSVLGPHPYPTMVRDFQAVIGREAR
                     EQVLSAEGRLPDLLVACVGGGSNSIGLFHAFLGDAAVRMVGVEAAGEGIATGRHAARF
                     AEPAEGILHGTRTLVLQDAAGQVRETHSISAGLDYPAVGPEHAMLGAAGRVVYDAATD
                     AEALAAFSALATEEGILPALESSHALAYVLREGVSGAIPAASVVVVNLSGRGDKDLSV
                     VEGLLGRDFGHVGA"
     gene            19465..>22627
                     /locus_tag="VFM00_02455"
     CDS             19465..>22627
                     /locus_tag="VFM00_02455"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HET7497134.1"
                     /translation="MKFGFRHLILSALLLLAFATNAKAQYLWMDTNGDGVNTSADVMS
                     PNGTGTTVDVWLNTAHNKDGSAAVCKSGDPDATLQTWNSFATHINATGGTVTYTGYTN
                     QVAAFTITCSNGGVAFNANTTEMAQCQATGTPVGNGGVNIKLFTVTVTGQTGTPSLAF
                     VPTNTLDNNPTSFGSPCGGLDFDNTIKLGTDFLDADGIGPAAGGNASPVISAPTTATG
                     TEGQVFTLSATATDADAADNLSLTATGQPASLTLTPGSGVSPVTATLNGTLGFGDQGN
                     YSIVWSVTDGHNPAVTATTNLTVTNVDRAPAVTAPATASGAENTLITYTVSAADPDGD
                     AILSLTAAPLPTGATFTANATKTAGTFSWTPSFSQSGSYNVTTTATNALSGSATTAIT
                     VTNVDRAPTVAAPATASGASNSLITFTVTASDPDADPITSLTAAPLPTGATFTDNATH
                     TSGTFSWTPTNAQTGTTNVTFTAANALTGSATTAITVTSGDRPPAVTAPATASGNENT
                     LITFTVTASDPDGDPIASLVAAPLPSGATFTANGSNTSGTFSWTPSFTQSGSYNVTFT
                     ATNALGGSATTAITVNNVDRAPAVTAPATASGAENTLITFTVTASDPDGDAITSLTGA
                     PLPSGATFTANGSNTSGTFSWTPSFTQSGSYNVTFTASNALNGTATTAITVTNSDRAP
                     VVTAPPTASGQVGVLLTFTVSASDPDGDAITSLSGAPLPSGATFTANGTNTAGTFSWT
                     PTTGQAGNYSVTFTAANALTGSATTAITIAPPGDAPPQVSAPPTASGDENTLITFTVT
                     ASDPDADPIASLTAAPLPSGATFTANATNTAGTFSWTPSFSQSGSYNVTFTATNALTG
                     SATTAITVNNVDRAPTVTAPATASGDEGTLITFTVTATDPDGDAITSLTGAPLPSGAT
                     FTANASNTSGTFNWTPSATQSGVYTVTFTAQNALSGSGSTEITVNNVDAAPVVTAPAT
                     QSGPEGTTITFTVSATDPDGDMIVSLTGSPIPAGATFTVTGEGTPTATGTFSWPNATP
                     VGSYPITFTASNVLTGTATTVVNV"
BASE COUNT         3411 a         8100 c         7994 g         3122 t
ORIGIN      
        1 cttccgcaag gacctcgaga tcgaagaaga cctgatcgag gaggtggctc gcggcatcgg
       61 ctatgacgcc gtgcccgcga cggtcgcggc ggcgtccgtt ccggcgatcg ccgagtcgcc
      121 cgagcgcgcg ttcgcggcgc gcgtcgtgga cgtggcggtc gggctgggct tcgacgaggc
      181 gatcggggcg gccctggtgg gcgacattcc cgccgaagcg cgcgagggca tcgaggctgc
      241 gagcatctgg gagatccaga atccgaagag ccgcgagctg aagcatctgc gcgtgagcct
      301 gcttccgggc ctgctcgcca tcgccgcccg gaacgcgcgc cacggcgtca ccgacgtgcg
      361 cctggccgag gtggggaagg tgttccaggc ccaaccgccg ccgctgggct ccgagcgtct
      421 cgaggcggcg ttcctcctca ccggctcgcc cgacccctgg aaccgccccg gcgccgactc
      481 cgaccgctac ctggagctgc gcggcgcggt tgaagcgctt tatgaagcat tgggaattga
      541 ctcatggcag ggcggctcct accatgatgc gtgctgggct cgcggaaccg gttccgtctg
      601 ggcggtttcg gggagccggc tcggccggat gggggaagtc gccccttcgc tggcccgcca
      661 gatgggcctg acgcagcccg catgggcggc gatcgtggat ctggcggcgg cgctccgcgc
      721 cgcgccccgg gagcggcgat accgcgaggt accgcggttc ccggcatcga agcgggatct
      781 ggcggttgtc gtgcgccccg acgtgacgca cgaggatctc gcggcgacga ttcgagcggc
      841 gggggggccg ctgctgcaca ccatccggct attcgacgtc tacaagttcc gcgacggaga
      901 gcacgcgggc cgctcgagcc tggcctacgc gctggagttc cgctcgccgg agcgcacgtt
      961 gaccgaccgc gaggtcgagg attcgttcgc ggcgatcgtg cgcgccctgg aatcgaagct
     1021 gggcgcggcg cttcgcggcg gagcggaggt gccccgcgcg tgagcgcgat cgtgacggaa
     1081 ctgtcggcgc tggaagaacg cgtacagcag atcgtgagtc tgctggaacg gatgcgcgaa
     1141 gagaatcgcg ccctgaccgg cgaacgggat gaccttcggg cgaaagtgcg ggcgctcgaa
     1201 gcggcggcgg gcaagcacgc cgcggccgac tacaagaccc gcttgaagtc gctcgaagac
     1261 gaacgggcga ccctgttgga agaacggcgg gcgctcgcgc ggcgcgtcga ggagatgctg
     1321 gcgaagctgt cggtcctcga gaaagccgtg cacgcctgac gccatcggcc ggtcccggac
     1381 atccgtcggg ggcggctgcc ggaaacgacg agaacgaaag gaccgaggtg ggatcggaac
     1441 cgacgaccca gagcgtgacg atcttcggca gcgagtacaa gatcaagggc gccgaccccg
     1501 attacatcca gaccgtcgcg gcatacgtcg acggaaagat gcgcgagctg gaacgcaggc
     1561 tcgcgacggg cactcccacc aaaatcgcca tcctcgcctc gctcaacatc gcggacgagc
     1621 ttctccggga acgcgccgag cgcggccggc tcgacaagga gctggccgag cgtaccaagg
     1681 cgctcacaaa aaccatcgga gattcgcttc gcgagcgctg agcccttccg gcggcgcgca
     1741 tccacgacgg aggtcaccga caccggccac gacgacgaac aaccgaaaac cctgccatgc
     1801 tcgtgtggtg ccggaatttt ttgagccaca actcaagact tgggagtctg gatacccggc
     1861 gtcgtggtgg tccgcctttg gcggacgacg caaacccggg cgaagggcac ccacctggtc
     1921 gctccaggtt caaaagtctc gcgcccacgg catcggcggg gctctttttg tatacggagg
     1981 atcgccgctc atgaccgtgt ccctgctgac gctgatcctc gccgccctcg gctcgggcat
     2041 cgtggcgttc ctgcttggat ggatcgccca ccggaagatc ggcgagggga aggtgctgcg
     2101 cgccgaagag ctggcggcca agatcctgcg cgaggccgag cgggaggccg agacccagaa
     2161 gaaaagcgcc ctcctcgagg ccaaggacga gtggttcaag gagaagtcgc gcctggaccg
     2221 cgaggcacag gcccagaaga acgagatcca gcgcgcggag cggcggcttc aagagctgga
     2281 gaccaacctg aaccggcgcg ccgaggtgct cgacaagaag gagcgggacc agaagcggct
     2341 ggaacgggac ctgtcggtca agggggaggc cctcgagctg cgcgacaagg agctcgagcg
     2401 ggtcctgggc gagcagaacg cgcggctcca gcgcatcagc ggcatgacgg ccgaagaggc
     2461 gaagcagctc ctcatcgtga acatggagaa cgaggcgcgc gccgaggccg gaaagctcct
     2521 ccagcagatc cgggaggagt ccacgcggaa cgcggagaag gaggcgcggg agatcgtcac
     2581 gctggcgatc cagcgctgcg ccgccgacca ctccgccgag accaccgtct cggtggtcca
     2641 tctccccagc gacgacatga aggggcggat catcggccgg gagggacgga acatccgcgc
     2701 cttcgagacc gtgaccggca tcgacgtgat catcgacgac acgcccgagg ccgtgatcct
     2761 ctccggcttc gatccggtgc ggcgcgagat cgccaagcgt tcgctcgagc gcctgatcac
     2821 cgacggccgg atccatcccg cgcgcatcga ggaggtcgtg gccaaggtgc agaaggagtt
     2881 ggaggagcag atcatcgagc tgggcgaggc cgccgtgctg gaagtgggcg tgcacggcat
     2941 gcacccggag atcgtgaagc tcctgggcag gctccagtac cgcaccagct atgggcagaa
     3001 catcctccag cactccaagg aggtcgcctg gatctgcggg atcatggcgg ggcagctcgg
     3061 cttcgacacg cagatcgcca agcgcgcggg actcctccac gacctgggca aggcggtgac
     3121 gcacgaggtg gagggagcgc acgcgcagat ctcgatggat ttcgcccagc gctacggcga
     3181 gccgcaggag gtggtccgcg cggtgggcta tcaccatcac gatcccacgg ccgagaacct
     3241 ctacgccgtg ctggtcatgg cggcggacgc cgtctccggc gcccgtcccg gcgcgcggcg
     3301 cgagagcatc gagaactacg tgaagcgcct ggaggcgctg gagaagatcg ccgactcctt
     3361 cccgggggtc gagaagtcct acgcgatcca ggcggggcgc gaggtgcgca tcatggtcga
     3421 gcccaagaac gtggacgacg cgcgcgcctc gcagctggcg agcgacgtcg cccgccgcgt
     3481 cgagcgggag ctccagtacc ccggccagat caaggtcgtg gtgatccgcg agacgcgcgc
     3541 catggaatac gcgcgctagg ccccgcgtga acatcctctt cgtcgcggac atcttcggtt
     3601 ctccgggccg ccgcgcggtc cgggatctcc ttcccgagat cgtctcctcc atgggcatcg
     3661 acctggtggt cgtgaacgtg gagaacgcgg ccgccggctt cggggtcacg aaagagatcc
     3721 tcgaagaggt gaaggggctg ggcgtccagt gcatgacgag cggcaatcac atctgggacc
     3781 ggcgcgaggt gatgcctctc ctggactccg agccgctcct gctccggccg cacaactacc
     3841 cgccgggcgt gccggggcgc ggcgtgatga tcgcgcgcac cgcttcgggc gcgaaggtcg
     3901 gggtgatcaa tctcatgggg cgggtcttca tgcgcgagct gctcgatccc ttccgggtgg
     3961 ccgaggaaga ggcggcgaag ctgcgcgagg agacgccgat catcctggtg gatttccacg
     4021 ccgaggcgac cgcggagaag gtggcgctgg ggtggcacct ggacggcaag gtctccgccg
     4081 tgatcggcac gcacacgcac gtccagaccg ccgacgagcg gatcctgccc ggcggcaccg
     4141 gctacatcac cgacgccggg atgaccggtc cgcacgactc cgtgatcggc gtgcgcaagg
     4201 accaggcgat ccggaagttc ctcacgctgc tgccgacccg cttcgagccc gcgacgggcg
     4261 acgtgcgcct ccacggcgtg cacctcgagg tggatgacgc cacagggcgc tgccgccaca
     4321 tcgagcgcgt ggcgatggcg ttggagccgg ctcccggggc cgccgtcccg tgaccaccgc
     4381 gccggccgcg gcgctcctcc tgggcggccc gctcgccgcc gaaatccgcg ccggggtgaa
     4441 gcggcgcctc gaagcgctcc gcgaccgcgg ccggcccgcg ggcctcgccg tcgtcacggt
     4501 gggagagcct ccttcgggaa acccctacgt gcgcgccaag gtgaaggcgg cccaggaagc
     4561 gggcgccgtc gccaccgtcc atcgcctttc cgaggacatc gccgaaccaa agcttcgcga
     4621 cgcgctccgc gcgatcggcg acgaccccgc ggtccatggc ttgatcctcc agcttccgct
     4681 cccgcgccat ctgcgcgagg agcgccacct ggaggacgtg cctccggcca aggacgtgga
     4741 cggtatccat cccgcgaacg tgggccggtg ggtgaacggg cttcccgcgc cgcgcccggc
     4801 cacgcccctg ggggtgcagg agctgttgcg gcgccactgc ggcgatctcg cggggaagcg
     4861 cgcggtcatc ctgggacgga gccgcatcgt cggacggccg ctctcggtgc tcctctcctc
     4921 ccgcgagccg gggatgaacg cgaccgtcac cctctgccac tcggagacgc gcgacctgcc
     4981 cgcgctctgc cgcgaggccg agattctcgt cgtggcgatc ggccaccgcc gctcgatcgg
     5041 cgccgcgcac gtccgcccgg gcgccgtcgt gatcgacgtc gggatccatc ccatcgccaa
     5101 tcccgccccc ggcgcgccgc gctatgaggg ggacgtggac ttcgaggcag tgcgcggcat
     5161 cgcgtcggcg atcacgccgg tgccgggggg tgtcggtccg ctgaccgtcg caatgctgct
     5221 gcgcaatctg gcggacgccg cggaagcggc ggaagctgcg gagggcggcg gatcatgagc
     5281 gcgcgccgct cgtcgagtct cgcctctcgt gtcttggggt caacacggat gggcgccggc
     5341 cccggtgcgg atgtctgcct cggtgcggag gcccgcttcg ttacggaaat ccgccgcggt
     5401 gcggaggtcc gccttggaga ccccacgtcg cgctcctcga gtcgtacaga gagagcgaca
     5461 gatgtcttcg ctttcaccgc cgctagcggc ggtaaaatcc tgaccacgtg gcgggacaga
     5521 ggctcgcgcc caaggatccc gacggccata cgcagacgct cgcggccaag gaccgcgacg
     5581 gccgtacgat gagcgcgcga cgcaattggg gcgccgccgc ccgcttcgat ccgcgcgaag
     5641 gagagccgca cgtctacacc gtcggcgaga tcgtcgccga gctgaatgcg ctcctcggcg
     5701 gggaataccc ctcgatcacc gtggaggggg agatcaccga cttccgcgcg catccctccg
     5761 gacacgaata cttctgcatc aaggaccgcc gggcgcagct ccaggtggtc ttcttcgcca
     5821 gccaaccccg catccgcggg gtccagctca agaacgggct cgccgtacgc gtcaccggca
     5881 ccatcacggt ctacgaagcg cgcggaagca tgcagctcaa ggcggagcgg gtgtcgccgg
     5941 tcggtttcgg cgccctccag atccgcttcg aggagctgaa gcgaaagctc caggccgagg
     6001 ggctcttcgc cgtggagcgg aaacgggagc tcccgcgcta cgccacccgg gtcgccatcg
     6061 tcacctcctt ctccggcgcg gcgatccgcg acatgctccg gatcctgcgc cagcgcgcgc
     6121 ctcacgcgcg gatcaccgtc gcgccggcgc gcgtgcaggg agacggcgcc gcgctttcgg
     6181 tcgcggccgc gatccggctc gtgaacgact ggggtgacgc cgacgtgctg atcgtggggc
     6241 gcggcggggg ctcgctcgag gatctctggg ccttcaacga agaggcggtg gtgcgcgcca
     6301 tcgtcgcctc gcgcatcccg gtcatctcgg ccgtgggtca cgagtcggat atcacgttgg
     6361 ccgacctcgc ggcggacgtt cgcgccgcga cgccgacgca cgccgcccag caggccgcgc
     6421 ccagccggga agaggcccgc cataagctcc accagctcgc cgttcacgcg cgggagcggc
     6481 tgctgcgcga gatgcagcgc gaccgccgcc acctcgacgg ctaccgcacc caccgcgcgt
     6541 tccgcgagcc gcaccggcag gtcgccgagg gacggcagac cctcgacaat acccgcgacg
     6601 tcctcgcgcg cgcgctcgag ggctgggcgc ttcgccggcg cagcaccgtc ggttcggcct
     6661 ccacgcggct tcgcgcgcac gctcccgccc gggtcgtgga gcggatgcgg gagcgcctgc
     6721 tggagctgcg ccggcgcgcc gaacgtgcgt cgataaccga tctggcgaag cgccggaagg
     6781 aggcgggcgc ccgcgcgcgg ctcctcactt cctacgacta ccgcggcgtc ctccggcgcg
     6841 gctacgcgct ggtctggaac gaggagggcg cgcgtctcgt gaaccgcgga cggaaccttc
     6901 gccccggcga ggggattcga gtacagttcc acgacgcccg cgcggaggcc tcggtcacgc
     6961 gcgtggatcc gtcgaccgag gaggaaacgc catgaagcgg aaggaagggc cggcccccga
     7021 cgcgcccacg ttcgagagca tgatggagcg gctcgagcag ctggtgggga cgctcgagtc
     7081 cggcaacctg agcctcgagg agtcgatcca ctccttcgag gaggggatgg cgctcgtgaa
     7141 gcggtgcacc gaggtgctcg accaggcgga acagcggatc cagaagctca cgcgcgacgc
     7201 ccagggggcg ccgcggacgg aaccggtcgc ggaggacgac gaggcggatg aagggcggag
     7261 cggcgagctc ccgttttgag cgctacctcg agcgcgcgcg tcctcggatc gaccgcgcgc
     7321 tcgacggagt gctcccgcgc gcgtcggccg agccccgccg gctgcacgcg gccatccgct
     7381 acagcgtctt cgccggaggg aagcggctcc gccccgcgct ctgcctgctc gcgtgcgagt
     7441 cggtctccgg ctcctgcagc gacgcgatgc atgccgccgc cgcgctggag atgatccaca
     7501 ctttcagcct gatccacgac gatctccccg gcatggacga cgacgactgg cgccgcgggc
     7561 gcctcaccaa ccacaaggtg ttcggcgagg ggatggcgat cctcgccggc gacgcgctgc
     7621 tcgcgctcgc gttcgagacg ctggcgacgc gccccacggg gcgcgccaag gatccgcgcg
     7681 cgctcctgcg aaccgtctgc gaggccacgg gccacgaggg gatgatcggg ggccaggcgc
     7741 tggacctgct ctacgagcgg cagcgggtct ccttcgcgcg agtgctgcgg atgcaccggc
     7801 ggaagacggg gatgctcctt cgcggctcgc tcctcgccgg cgcccaggtc gcgggcgcga
     7861 gcgcggcggc gctccggcgc ttcggcgact acggcgaccg gatcggcgtc gcgtttcaga
     7921 tcaccgacga catcctgaac gagcgctcca cgaagcgggc cacggggaag agcgtgcgtt
     7981 cggaccgcgc gcgcggcaag gcgacggcgc cctcgagcgg cggcatggcg cgcgccgagc
     8041 gcgaggtcga gcggctcctc gacgaagcgg ccgccatcgc cccccggctc ggccgccgcg
     8101 ccgaggagtt cgcgtcgctc acggaattcc tccgccatcg gacgcgctga cgcatgaacc
     8161 tggcgcccca gggaggggag agctgatggg tttcaccgcg aatccgctgc tgcacgcgct
     8221 gctctgcggc ttcctcgtgc agctcagcaa ggtgttgacc ttcctcctgc aagagcggaa
     8281 aatcaacgtg cggcggttcg tggaaaccgg cggcatgccc agcagccacg cggcttcggt
     8341 gatggccctg accacctgtg tggggctgcg cgaaggcgtt cggtccgttc tcttcggcgt
     8401 cgtcctgtac ttcagcctca tcgtgatgta cgacgccgca gggcttcggc gggctgcggg
     8461 ccgtcaggcg accctgctca accggatcct gcacgagcac ctgcagctat ccggaccgcc
     8521 cgcggagcga ctgcgcgagc ttctgggaca tacgccgatc gaagtcttgg tgggcgcctt
     8581 gatcggcgtc ctgttctcgt tcgccatcta tcgccccccg ggaggaccgt gagcaccgcc
     8641 agcgcatcca tgaacgcctc cacgaaccac ctggccggaa tctcgagccc cgaggacctg
     8701 aagcgccttc cccccgaggc cctcccggcg ctcgccgcgg aaatccgcga gcggatcctc
     8761 tccgtcgtct ccaagaccgg cggccacctg gctcccagcc tcggcacggt ggagctgacg
     8821 atcgcgctcc actacgtctt ccacaccccc gaggacatcc tgatctggga cgtgggccat
     8881 caggcctacg gccacaagat cctgaccggg cggaacgacc gcttcgacac gatccgccgc
     8941 gagggcggcc tgtcgggctt tccgcgccgc gtggagagcc cctacgaccc gttcggcacc
     9001 gcgcacgcct ccaccgccat ctccgccgcg ctcggcttcg cctgcgcgcg cgacttggcg
     9061 cacgagaagc gccgcgtcgt cgcggtcgtg ggcgacggag cgctgaccgg cggcctggcc
     9121 tacgagggga tgaaccaggc gggcgcggcc ggcaccgatc ttctcgtcgt gctgaacgac
     9181 aattcgatgt cgatctcgcc gaacgtgggc gcgatctcgc agtacctgac gcgcctgacg
     9241 gcggggccga tcttccgccg cctcgaggcc gacctgtggg acctcctcgg caagctcccg
     9301 gccggcggca cggcgcgcaa gctcgcgggc cgcatcaagg agagcatgaa gaacctggtc
     9361 gtgccgaacg tcctcttcga agagctgggc ttcaagtact tcggcccgat cgacggacac
     9421 gacctgacca cgctgctcac agtgctgaac caggtgaagg aggtgaaggg accggttctc
     9481 ctccacacgc tgacccggaa ggggaagggg tacggccccg ccgaggagga ctcgcgcaag
     9541 ttccacggcg tctcctcgtt cgacaagctg accggagcga gcgcccggaa gacgtccggc
     9601 cccgcctaca ccgagatcta cggccagtcc atgatcgagc tggcgcggca ggatcccaag
     9661 atcatggcca tcacggccgc catgaccgac ggcaccggcc tcacgaagtt cgcgacgacg
     9721 ttccccgacc gcttccacga cgtcgggatc gccgagcagc acgcggtctg cttcgccgcg
     9781 ggcgtcgccg ccgcggggct ccgccccttc gcgacgatct actccacctt cctgcaacgg
     9841 gcgtacgacc agatcgtgca cgacgtcgcg gtgcaggggc tgcccgttcg cttcgcgctg
     9901 gaccgcgccg gactggtggg cgaggacggc tcgacccatc acggtgtctt tgacatcggc
     9961 tatctccgct gcctgcccgg gatggtgctg atggccccca aggacgagaa cgagctgcgc
    10021 cgcatgctcg tcacgatggc cgcgcacgac gcggggccga tcgcggtgcg ctatccgcgc
    10081 ggcgcgggtg tgggcgcgac gctcgacccc aatccgcagc cgctcgcgat cggcgaggcg
    10141 gagctgatcc gctccggcaa ggacgtcgtg ctcgtcgcct acggcgcgat ggtcgccgtg
    10201 gcggagaaga cggcctcgct cctcgccgaa tcgggcatgg acgccgcggt gatcaacgcc
    10261 cgcttcgcca agccgctcga cgaggcgatg ctcctcgact gggcgcgccg cgtcccgcgg
    10321 ctggtgacgc tggaggaggg ggcgctcccc ggcgggttcg gcgacgccgt cctcgagctg
    10381 ttcgcgaaga ccacggttcc cggcgtccag atccgcacgt tcggcgttcc cgaccgcttc
    10441 ttcgaccacg gctcgcgcga gtcgctcctc ctggccgcgg ggcttcatcc cgacctgctc
    10501 gccccggaga tccgccgctt cctcgccggc gatccacgct cgaccccgga accgctcgcc
    10561 cccaaggcat ccagcgctcc catcgccacc cacgcatgat ccgcccgccc cgtcacctgg
    10621 ggatcgtcgg caatcgcggg aaggaggggg tgcgcgcgct cgtgccgcca ctcatccgct
    10681 gggcccgcgc gcaagggcgg aaggtctcgc tcgaccggga gctggcccgc gggatgcgcg
    10741 gcgtcggcgc gggaatcacg ctggaggcgc tggtaaagcg cgccgacgcg atcctcgtgc
    10801 tgggcggcga cggcaccatg ctcggcaccg cgcgcgccgc ttccgccgcg ggcgttccaa
    10861 tcctcggcgt gaacctgggc ggtctgggct tcctgaccga gaccgcgcag gaggacctct
    10921 atcccgccct ggagcgcctg ttccggggcg acgtgacgct ggagcggcgg acgatggtcg
    10981 aagcgcaggt gcggcggcgc gggagcaagg gcgtgtggca ggaagtgggg ctgaacgacg
    11041 cggtgatcca tccctccgag cgctcgcgcg tgatcgcgat ggacctgcgg atcgcgggca
    11101 aggagatcgg aacgctggtg ggggacggcc tcatcgtcgc gagcccgacc ggctcgaccg
    11161 cctactccct ctcggcgggc gggccgatcg tccgtccttc ggtcgaggca ctcctcgcca
    11221 cgcccatcag cccccacacc gtgacctgga ggccgctcct ggttggcgcc gacgagacca
    11281 tccgcgtgcg cctgcgccct ggccatccgc gcgccaacct cacgatggac ggccaggtgg
    11341 cgcgcaccgt cacgcccgat gacgagatcc tgatccgccg cgcgcgccgc cgcgcgaccc
    11401 tcatcgtgct cgagcccgag tccttctacg acgtcctccg caccaagctc gcctgggcca
    11461 cgtcgccgcg aggacgggtc cccgaccaat aggcggatct cgctcccatg ctccgctggc
    11521 tctccatcca gggtctggcc ctcgtcgact cggtcgagct ggagttcgct cccggcttga
    11581 acgtgctcac gggagagacg ggtgcgggca agtccctcgt cctgggatcg atcgggctcc
    11641 tgctcggcga ccgcgccgac tcggcgtggc tccgctcggg cgaatcgcgc ggatcggtgg
    11701 aggggacgtt cgacctcgcc gggcgccccg acctgatcga agcgctgcgc gcgcacgacg
    11761 tcgagccgga agagggccgc gtcgtgctgc gccgcgaggt ggccgcgaat ggaacgacgc
    11821 gcgcgttcgt gaacgggcgg ggcgtcctcc tcgtccagct tcgcgccgtg ggcgacctgc
    11881 tcgtggacct gcacggccag cacgagcacc agcagctcct cgatcccgcg cggcaggcgg
    11941 acttcttcga cgcctgggcc gggacgtggg aggaacgccg cgcgctggag gccgagcgcg
    12001 ccgccatcct cgaagaccgg cgggccgcgg ccgcggcgcg cgcggccctg gagcgcgatc
    12061 gcgccgagga ggagacgctg cgcgccgacc tcgccgagct ggaggcgctg aagctcgagg
    12121 acggggaaga ggagacgctc ctccgcgacc gggagcggct ggtccacaag gagcgcatcc
    12181 tccaggggct gagcgaggcg ctggccctgc tggccgacga ggagaccggc gccgagtcgc
    12241 gcctccgccg cgcggtgaag gcgcttcgaa cgggcgccgc gctcgacgcg gggctcgagc
    12301 cgctggtcga agaggccgag gcggcgtcgg aggccgcctc cgccctgtcg tcgcgcgccg
    12361 agggggagcg ggcgcggctc ctcgaagagc cgctcgatct cgatcgcgtc gaagagcgcc
    12421 tggaccggat ccatcgcctg aagcgcaagc accggacgga cgcttcgggc ctcctcgccc
    12481 tggctgggga gctgcaaacg cgggtgcgcc agcttgcgcc cggagccgaa gaggtggatc
    12541 ggctcgagcg ggcgctcgcg gcgcggaccg ccgcgtacgg ccggaggctc gacgcactct
    12601 ccgagcgccg ggcgacgcgc gcggccgcgt tcgagcgcgc ggcgggtgct ctcctcgacc
    12661 ggctggggtt cggcaaggcg cgcctcggcg cggcgccctc cggcgcccga gcaggcgacc
    12721 ggagccggcc cgcgattgac cccgctccga tccccgctct agaattcgag ttccagccga
    12781 atcccggcga acccccgcga ccgcttcgca gaatcgcctc gggaggggag ctctcccggg
    12841 tcatgctcgc cgtgaagtcg ctcatggcgg acaaggacca ggtcgccgtg ctcgtcttcg
    12901 acgaggtgga tcagggaatc gggggagccg tgggcgagga ggtcggggcg ctgctccgcg
    12961 acctcggcga caagcgccag gtgctctgca tcacgcacct tccgctgatc gcggcctacg
    13021 cccagcggca gctccaggtg gcgaagtcgg tggcgcgggg aaggacgacg acgacggtgg
    13081 caccgctctc cgaagaggag cgcgtcgagg agatcgcgcg gttgctcgcg ggcgcccgcg
    13141 ccggcgagac gacgcgccgg caggcccggg agctcctgag cggggcccga tctggggccg
    13201 gcgccgccgc cggaccgtcc cggaagtgcg cgagtgcgcc gcagggcgcg gtctcacgcg
    13261 ccagaaccgc taaaggaaaa cgatgatcac tccgagcctc gaggactatc gcgcgctcgc
    13321 gcgccgctac aacctggtgc cggtcatgcg cgaggtgctg gcggacttcg acacgccggt
    13381 ctcggcgttc gccaagctga accgcggcga cgcgtcgttc ctcctggaga gcctcgaagg
    13441 gggcgagacg tgggggcggt attccatcct cggcttccgc ccctccatcg aattccgcgc
    13501 caagggcgcg gtggtcgaga cgcgccgcgg cgagcggacc gagcgcaccg agtcccagga
    13561 cccgctggag tccttgcggg cgctcctcca gtcggtgagc gccgccccgg ttgcgaacct
    13621 ccccccgttc agcggcggcg cggtcggact cgtgggctac gattacgtgc gtttcctgga
    13681 gcggatcccc gcgcgcgtgc ccgacgatct ggggcagccc gacctccatt tcgtctttcc
    13741 cgatctcgtg ctcgcgttcg acaacttccg ccatcgtctc cagctggtcg cgaacacgcg
    13801 ccccgggaac gatcccgacc gcgcctatcg cgacgcggtc gcggccatca gcgagatgct
    13861 atcccgcctg gcgcagccgg cgccggcgcc cgccgcgacc gagcgcgcga acggggaggt
    13921 ggccttcacc agcaacctcg gccgcgaggg ctacgcgcag gccgtcctcg ccgccaagga
    13981 gcacatccgc gcgggggaca tcgtccaggt cgtattggcg caccggctgg aggcgaccgc
    14041 ggcggtcagc cccttcgacg tctaccgcgc gctccggatc ctgaacccct cgccctacat
    14101 gtactacctc cgctactcgg atcgctcggt cgcgggatcg tcgcccgaga tcctcgtccg
    14161 cacctcgggc cggaagatcg ctctccgccc gatcgcgggg acgcggccgc ggggccagag
    14221 ccgggaggag gacgcggcgc tggagcagga gctcctcacg agcgagaagg atcgcgccga
    14281 gcacgtgatg ctggtggacc tggggcggaa cgatgtgggg aaggtggccg agatcgggag
    14341 cgtgcgcacc acggagttca tgaccgtgga gcgctactcg cacgtgatgc acctggtgag
    14401 ccatatcgag ggcacgcttc gggacgggct cgggcccttc gacgtcctgc gcgcctgctt
    14461 cccggccggg acggtgagcg gcgcgccgaa gaagcgcgcg atggagatca tcgaatccct
    14521 cgagccctcc cgtcgcgggg cctacgcggg cgcgatgggc tatttcgact tccacggcaa
    14581 cgcggatttc tgcatcacca tccggaccgc gaccttcgaa gggggccgcg tccacctggg
    14641 cgtgggggcc ggcatcgtgg ccgactccga tcccgaggag gagtggaccg agacgcgcaa
    14701 caaggggcgc gccgtggaag cggcggtgcg cctggccgcg ggaggactgg acgcgtgatc
    14761 ctgatgatcg acaactacga ttcgttcacc tggaacctgg tccagtacct gggggagctc
    14821 agggagaccc cgcgcgtcgt ccgaaacgac gagatcaccg tggacgaggc gctcgcgatg
    14881 aagcccgacg ccatcgtcat ctccccgggc ccgggccgtc ccgacgacgc ggggatcagc
    14941 aaggagctga tccggcgtag cgccggagtg gtgccgatcc tgggcgtctg cctgggccac
    15001 cagtgcatcg gcgaagtctt cgggggcacg atcgtgcggg ccgagcgcct cgtgcacggg
    15061 aagacgtcgg cgatcatcca cacggggcgg ggcatcttcc tcgacgtgga caatcccttc
    15121 atggcgaccc gctaccactc gctgctggtc gcgcgcgagg ggctccccga cgcgctcgcc
    15181 gtcgtcgcct ggacgcccga gaacgagatc atggcgctcc gccacaagga ccatgagacg
    15241 tggggcgtgc agttccaccc cgagtcgatc ctgacggccc acggcaagga cctcctccgg
    15301 aacttcctgc gcctcgtccc ggacgcggcg gcggcccgcc cggcggccgc ggcgcccgcg
    15361 cgctgacccg gatctccccg tcgatgacgt cccccgcgcg cgccgccgca tccctcgctc
    15421 ccttcgcccc cggcccccca agcggcgccc ccggctcatg atccgcgatc tgctgccgcg
    15481 cctcctcgcg ggagagaccc tgagccgcgc cgaggccgcc gcggcggtgc ggagcatggt
    15541 gcgcggcgag agcgaggacg ggctggtcgc gtcgttcctc accctgctcg cgcagcgcgg
    15601 ggaaaccgag gccgagctgt ggggcggggc cgaggcgctg cgcgccgagg cggtcccgtt
    15661 cccttggaac ggcggagcgc tcttcgatac ctgcggcacg ggtggggacg ggcgcgggtc
    15721 gttcaacgtc tccaccacgg cggcgttcgt cgtggccggc gccggcgtgc gcgtcgccaa
    15781 gcatgggaac cgctcggtct cgagcgcgtg cggcagcgcg gacgtgctgg agtcgctggg
    15841 cgcccacatc gatctcggcc ccgagggcgc cgccgccgtg ctcgccgaaa cgggattcac
    15901 ctttctctac gcgcgccgct tccaccccgc gatgcgccgg gtggcgggcg tgcggcagac
    15961 cctcgggttt cgtaccctgt tcaactggct cggtccgctc tccaatcccg cgcgcgccac
    16021 gcaccagctg gtcggcgtgc ccgatgcggc ccgcgtcgag ccggtcgcgc gcgtgctcgc
    16081 gggcctcggc gcggtgcggg cgctggtcgt gcacggcgcg ggagggctcg acgagctcgc
    16141 gctcgcgccc ggaaacctcg cggccgagtc cgcgcgctcg gagaccggcg cgcccgccgc
    16201 gctgccgcgg tcgatccgtc acgacgcgct gggactccgc gacgcgccgc tcgaggcgct
    16261 ccgtggcggc gacgccgacc ggaacgccgc gctcctgcgc gaggtcctgg cgggagagcc
    16321 ggggccgcga cgggacgccg tgctcctgaa cgccgccgcg gcgctctgga tcgccggcgc
    16381 cgctccgacc ctggacgatg cgctcgcccg cgcgacccag gcgatcgatc gcggcgacgc
    16441 gcggaaagtc ctggatcatt acatcgagct ctcgaagaag gtggggagcg agtgagcgac
    16501 ttcctcgcca cgatcgtggc cgagcgcctg gatcgcgtgc gccaggacgc ggagcggggc
    16561 ccctcgcgcg ccgcgctgcg ggccgaggcc gaggcccggc gcgacgagcg gcgtccgttc
    16621 ctgtccgcgc tccggcgcga ggccggcgcg ccgatccggg tgatcgccga ggtgaagcgc
    16681 gcctcgccct cggcgggcgt cctccaggcg gagtacgatc cggcgtcttt ggcgcgcgcc
    16741 tacgacgaag ccggcgccgc ggcgatctcg gtgctgactg agccggcccg cttcctgggc
    16801 tccctggagg acctggccgc ggtccgggcg gcggtcgggc tcccggttct gctcaaggac
    16861 ttcgtcgtgc acgagcggca gatctacgaa gcgggcgcgc gtggggcgga cgcggccctg
    16921 ttgatcgtgg ccgcgctcga cccgcgccag ctgggcgatt acgcggcgct attggtcgag
    16981 ctgggcatcg cgcccctgat cgagatccac gaggctcgcg agatcgacgc ggcgctcgcg
    17041 gcaccggggg ccctcggcgt caacaaccgg gatcttcgca ccttgacgat gcgccccggt
    17101 cacgccgaag ggatgctgcc gctcctgccg cccgatcgcg tgcggatcgc ggagagcggc
    17161 tatcacgatc gcgcggcggt ccagcgggtg gaacggctgg gcgccgacgc cgtgctggtc
    17221 ggagaggcgc tgctccgggc ggagcgtccc tcgcaagcgt tcgcggcgct gttcggcacc
    17281 gggccctcat cggaggagga gtcgtgagga cgcgcatcaa ggtctgcggg atcacgagcg
    17341 ccgaagacgc gcgtcaggcg ctcgacgccg gcgccgattt cgtgggggtc gtgctcaccg
    17401 aatcgccgcg gcgggtggac gcggcccgcg cccgcgccat ccgggagtcg ctcccggccg
    17461 ggacgccgct cgtcggggtc ttcgccgagg agccgccgga gacggtggcg ccgctcgccg
    17521 tggagctcgg gctccacgcc gttcaggtgg cgggttggct ggagcgggaa gagcgcttcc
    17581 ccttcgaggt ctggcacgtg ctgcgcggca cgaccctccc cgatcccacg acgctgccga
    17641 tgattccgct ccggacgtac cacctggacg cctacgatcc cgcccgcccg ggcggcacgg
    17701 ggcggctcgc cgactgggcg tgggccgcct ccgcggtcga ggtcgggcgc cgcctgatcg
    17761 tcgccggggg actcactccc gagaacgtgg ccaccctggt gcgggacgtt cggccctacg
    17821 gcgtggacgc ttcgagcgga ttggagtccg cggtggggaa gaagagcccg gagaaagtgc
    17881 gcgccttcgt cgagcggatc cgcgccgcgg accagtctcg ccccaagcgg tcatgagcgc
    17941 gcgcgcgaat ccgcagggcg aagggcgctt cggcggctac ggcggacgct acgtcccgga
    18001 gaccctcctc ggcccggtgc tggagctggc ggaggcgtac gaggccgcgc ggcaggaccc
    18061 cgcgttccag gcggagctgg aatcggagct ccggagcttc gcgggccgcc cgacgccgct
    18121 cgcgctggcg ccgcgcttcg ccgcacgcgc cgggcttacg cgcgtctacc tgaagcgtga
    18181 agacctcctc cacaccggcg cccacaagat caacaacgcg atcggccagg cgctcctcgc
    18241 gcggcggatg ggcaagaagc gcctcgtcgc ggagacgggg gcggggcagc acggcgtcgc
    18301 cagcgccacc gcggccgcgc gctacggcct ggagtgcacg gtctacatgg gcgaggtgga
    18361 catggctcgg cagcgtccga acgtggagcg catgtggctc ctgggcgccg aggtgcgccc
    18421 ggtcgcttcc ggctcgcgca cgctgaagga cgccatcaac gaagcgatgc gcgactggtc
    18481 gggctcggtc cgcgacacgc actacctcct cgggtcggtg ctcggtccgc acccctaccc
    18541 gaccatggtc cgggacttcc aggcggtgat cggccgcgag gcgagggagc aggtgctgtc
    18601 ggccgagggc cgccttcccg acctgctcgt ggcgtgcgtc gggggaggga gcaactcgat
    18661 cggcctcttc cacgccttcc tgggcgatgc cgccgtccgc atggtcggcg tggaggcggc
    18721 cggggaggga atcgcgacgg ggcgccacgc cgcgcggttc gctgagccgg cggaggggat
    18781 cctccacggg accaggaccc tggtgctcca ggacgccgcg ggccaggtgc gggagaccca
    18841 ctccatctcg gcggggctcg actacccggc ggtagggccg gagcacgcca tgctcggcgc
    18901 cgcggggcga gtcgtctacg acgcggccac cgacgccgag gcgctggccg ccttcagcgc
    18961 cctggccacg gaggagggga tcctccccgc gctggagtcc agccacgcgc tcgcctacgt
    19021 gcttcgggag ggcgtatccg gagcgatccc cgccgcttcc gtggtggtcg taaacctgtc
    19081 ggggcggggc gacaaggacc tttcggtggt cgaagggctc ctcgggcgcg attttggcca
    19141 cgtgggggct tgaccggaac aggtgattct gctatcattt gccggtctcg tcagaccggt
    19201 tttctaggga cagcttgctc ggcatcggcg ggtcgtagag atcccccctc caggccgtca
    19261 tggcggccaa ccctccgcgc ttccgcgggg ccgacatagc ctcgtcctta ggtccgcgcc
    19321 gcccaccctc gggaggcccc ggaccgccgc acggctccgc gtgcggtcga aggagtcctg
    19381 cacaacagtg gagacgcagc ctcaagaagg accctttcgt gactgggcaa ggaaccccct
    19441 cgtgtatccc ctaaggaggc ctgcatgaag ttcggattcc ggcatctcat cctgtccgca
    19501 ttgctgctcc tcgcgttcgc cacgaacgcg aaagcgcagt acctgtggat ggacaccaac
    19561 ggcgacggcg tgaacacctc cgcggacgtc atgagcccga acggcacggg aacgaccgtg
    19621 gacgtctggc tcaacacggc tcacaacaag gacgggtcgg ccgcggtctg caagtcgggc
    19681 gatcccgacg cgacgctcca gacctggaac tccttcgcga cccacatcaa cgccaccggc
    19741 ggaacggtga cgtacaccgg ctacacgaac caggtcgcgg cgttcaccat cacgtgctcc
    19801 aatggcggcg tggcgttcaa cgcgaacacc acggagatgg cgcagtgcca ggccaccggc
    19861 acgcccgtcg gcaacggcgg cgtgaacatc aagctgttca cggtcacggt gaccgggcag
    19921 accggcaccc cgtcgctcgc cttcgttccg accaacaccc tcgacaacaa ccccacttcc
    19981 ttcggcagcc cgtgcggtgg gctcgatttc gacaacacga tcaagctggg caccgacttc
    20041 ctggatgccg acggcatcgg cccggccgcg ggcggcaacg cgagcccggt gatcagcgcg
    20101 ccgacgacgg cgaccggcac cgaggggcag gtcttcacgc tctcggcgac ggcgaccgac
    20161 gcggatgccg ccgacaacct ctcgctcacc gcgacgggcc agccggcgtc gctcacgctc
    20221 acgcccggct ccggcgtttc gccggtgacg gccacgctga acggcacgct cggattcggc
    20281 gatcagggga actactcaat cgtctggtcg gtcaccgacg gccataaccc ggcggtcacg
    20341 gcgaccacga acctgaccgt cacgaacgtc gaccgggcgc cggcggtcac cgctccggcc
    20401 acggcgtcgg gcgccgagaa cacgctcatc acctacaccg tctccgccgc cgatccggac
    20461 ggggacgcca tcctctcgct gaccgcggcg ccgctcccga cgggcgctac gttcaccgcg
    20521 aacgccacca agaccgcggg gacgttcagc tggacgccgt cgttcagcca gtcgggcagc
    20581 tataacgtca cgacgaccgc gacgaacgcc ctgagcgggt ccgccaccac ggcgatcacg
    20641 gtcacgaacg tcgatcgcgc gccgacggtt gcggctcccg cgacggcgtc cggggcctcg
    20701 aactcgctca tcaccttcac cgtcaccgcc tccgatccgg atgcggaccc gatcacctcg
    20761 ctgacggcgg ctccgctacc cacgggcgcg acgttcacgg acaacgcgac ccatacctcg
    20821 ggcacgttca gctggacgcc gaccaacgcc cagaccggta caaccaacgt caccttcacg
    20881 gccgcgaacg cgctcaccgg ctcggccacg acggcgatca cggtcacctc gggcgaccgc
    20941 ccgccggccg tcacggctcc ggcgaccgct tccggcaacg agaacacgct gatcacgttc
    21001 accgtaacgg cgtccgatcc cgacggcgat ccgatcgcct cgctggtcgc ggcaccgctt
    21061 ccttccggcg cgacgttcac ggcgaacggc tcgaacacgt cggggacgtt cagctggacg
    21121 ccgagcttca cccaatcggg gagctataac gtcaccttca ctgccacgaa cgcgctgggc
    21181 ggatcggcca cgacggcgat cacggtgaac aacgtcgacc gcgctccggc ggtgacggct
    21241 ccggcgacgg cttccggcgc cgagaacacg ctcatcacgt tcaccgtcac ggcttccgat
    21301 ccggacggcg acgcgatcac ctcgctcacg ggcgctccgc tgccctcggg cgcgacgttc
    21361 acggcgaacg gctcgaacac gtcggggacg ttcagctgga cgccgagctt cacccagtcg
    21421 ggctcgtaca acgtgacctt cacggcgtcg aacgcgctca acggcacggc gacgacggcg
    21481 attacggtca cgaactccga ccgggcgccg gtggtcacgg ctccgccgac cgcttcgggc
    21541 caggtcgggg tcctgctcac gttcacggtg tcggcctccg atccggacgg cgacgccatc
    21601 acctcgctct cgggcgcgcc gcttccctcg ggcgcgacgt tcacggcgaa cggcacgaac
    21661 acggccggca cgttcagctg gacgccaacg acggggcagg ccggcaacta cagcgtcacc
    21721 ttcacggccg cgaacgcgct gacgggctcg gccaccacgg cgatcacgat cgcgcctccg
    21781 ggcgatgcgc cgccgcaggt ctccgcgccg ccgacggcgt cgggtgacga gaacacgctg
    21841 atcaccttca cggtgacggc gagcgatccg gacgccgatc cgatcgcctc cttgaccgcc
    21901 gctccgcttc cttcgggcgc caccttcacg gcgaacgcca cgaacacggc gggcacgttc
    21961 agctggacgc cttcgttctc ccagtcgggc agctacaacg tgacgttcac ggcgacgaac
    22021 gccctcaccg gctcggccac cacggcgatc acggtgaaca acgtcgaccg cgctccgacg
    22081 gtcacggctc cggccacggc ctccggtgac gaggggacgc tgatcacctt cacggtcacg
    22141 gccaccgatc cggacggcga cgccatcacg tcgctgacgg gcgcgccgct tccttcgggc
    22201 gccacgttca cggccaacgc ctcgaacacg tcgggcacct tcaactggac cccgtcggcg
    22261 acgcagtccg gcgtctacac cgtgacgttc acggcgcaga acgcgctctc gggctcgggt
    22321 tcgacggaga tcacggtcaa caacgtcgac gccgcaccgg tggtcacggc tccggcgacg
    22381 cagtcgggcc ccgagggcac cacgatcacc ttcacggtat ccgcgacgga tcccgatggt
    22441 gacatgatcg tttccctcac gggctcaccg atcccggcgg gagcgacctt caccgtcact
    22501 ggtgagggca cgccgacggc caccggcacc ttctcctggc ccaatgccac gccggtgggg
    22561 agctatccga tcacgtttac cgcctcgaat gtgctgacgg gcacggcgac gacggtcgtc
    22621 aacgtca
//
LOCUS       DASSLZ010000068         8602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1798041, whole genome shotgun sequence.
ACCESSION   DASSLZ010000068 DASSLZ010000000
VERSION     DASSLZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798041"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1570)
                     /locus_tag="VFM00_02460"
     CDS             complement(<1..1570)
                     /locus_tag="VFM00_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HET7497135.1"
                     /translation="MKLDAKVPPGPMETKWEKHRSSIRLVNPANKRKYTVIMVGSGLA
                     GASGAASLAELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVFRLFYDTVK
                     GGDFRSREANVYRLAEISVNIIDQCVAQGVPFAREYGGLLANRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQALERQVGLGKVEMFARHEMLELILIDGRARGIVTRDLVTGQVESHL
                     ADAVCLATGGYGNVFYLATYAKGANATAIWRAYKKGAGFANPCFTQIHPTCIPVTGEH
                     QSKLTLMSESLRNDGRVWVPKQQGDARAPALIPEEERDYYLERKYPSYGNLAPRDIAS
                     RAAKQVCDEGRGVGPGGRGVYLDFGDAIRRLGEPAIREKYGNLFEMYERITDENPYHV
                     PMRIYPAAHYTMGGLWVDYNLMSTIPGLHVLGEANFSDHGANRLGASALMQGLSDGYF
                     VLPYTIGNELAPMLNKPIPGTDHPAFAEAEREAADRYRGYLNIKGTRSPDHFHRELGR
                     IIWDYCGMERTEEGL"
     gene            complement(1575..2267)
                     /locus_tag="VFM00_02465"
     CDS             complement(1575..2267)
                     /locus_tag="VFM00_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HET7497136.1"
                     /translation="MERTQVGPGQFSRAEPFAASTIGKKVVMAVTGFILFGFVVAHMI
                     GNLQAYLGPEALNAYAVWLRELLHGAGLWIARAVLLLSVILHIWSATALTIDNRRARP
                     VGYRRREYDRSTYASRTMVWSGPILLLFIIYHLLHFTFGTVHPSFVEGNVYHNFVAGF
                     QVVPVSIFYIVAMLALGYHLYHGIWSMLQTLGLSHPRYNFLRHGFAGLMTAIVVVGNI
                     SFPLAVLTGVIR"
     gene            2349..2684
                     /locus_tag="VFM00_02470"
     CDS             2349..2684
                     /locus_tag="VFM00_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497137.1"
                     /translation="MSRTRAAWILAIVADTLQIVLLPAFGEGLASPAADALDIVVALG
                     MTVLLGWHFAFLPTALAEIVPGLNLVPTWTAAVFFVTRSRRARGSDIPEEPGRMQEPP
                     RGTLPPPPQ"
     gene            2841..3905
                     /locus_tag="VFM00_02475"
     CDS             2841..3905
                     /locus_tag="VFM00_02475"
                     /inference="COORDINATES: protein motif:HMM:NF020678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HET7497138.1"
                     /translation="MNHRGVAAAVCLTASLFLHVPAWGADPEPAPVATRGSSPRITEI
                     AIFRDAAIRWSPDSLRFFARRGLSYVDAGRGVVTTVALPTLRGPHRITAVLTVHPVPK
                     SDREVCDRYDRAGSIRMAVPGAPDLEVLRFITAYGGSTRHEVDVTALAPLLQGRRTFT
                     AHIDTWTSPAWTVDVSLRYTPVADYEDPTWAAPLYRTDNFNREAMPEGDSATIVIPPG
                     LARVVLRYTATGHCTDGVDADEFVSKANVIAVDGVVVARFHPWRDDCRSFRDRNPYCA
                     RWTDGRWSSDYARSGWCPGVEVLPQEFDLTDHLRAGRHTIRIAIEDMRPKDAKGNYGY
                     WRVAAALVGWKSPPRLWRNE"
     gene            3928..5229
                     /locus_tag="VFM00_02480"
     CDS             3928..5229
                     /locus_tag="VFM00_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7497139.1"
                     /translation="MSPPPRRARGGVRTIGRALAHRNYRLFFTGQSISLIGAWLTRVA
                     TSWLVYRLTHSAWMLGIVGFAGQFPTFLLAPIAGVWVDRWNRHRVLVVTQVLAAVQSG
                     LLAYLALRGIITIPHVVALSILQGVINALDMPARQSFVVDMIGNNREDLPNAIALNSS
                     MFNGARLIGPSVAGILIAWVGEGWCFFIDAVSYLAVIASLLMMKVALSERPRRESSVV
                     REIREGYRYIAGFTPIRSVLFLLALVSLLGFPYTVLMPIMAAQVLHGGPHTLGFLMAA
                     SGVGALTGALYLASRSSVLGLGSLIARSAAGFGVGLVLFSLSRNVWLSMALMAICGLT
                     MILQMAASNTVIQTLVDDDKRGRVMSFYSMAFFGTAPLGSLFAGTVATWIGVPRTILV
                     GGAACVLAALGFWKALPHIRRAARPVYVRLGILPAPTEQSL"
     gene            5258..6217
                     /locus_tag="VFM00_02485"
     CDS             5258..6217
                     /locus_tag="VFM00_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7497140.1"
                     /translation="MRTLALPTLDEVRAARERIRGVAIRTPLLRLNVDGPAEIWLKCE
                     NLQPIGSFKLRGAGNAMALLSREALERGVYTASAGNMAQGVAWCAKRLGIPCSVVVPD
                     HAPEIKTAAIERLGASVLKVPFASWWRVMTEHRHEGMKGTFIHPFSDPAVLAGNGTIA
                     LEVLEDLPEPDAILVPYGGGGLSTGIATVARALAPRARVHACEVATAAPLAASLAAGA
                     PRTVTYTPSFVDGIGGSGVFEEVWPHARALLAGSLVVSLEEAASALRLLVERNRLVAE
                     GAGAVPVAAALRGLEGARTIVCVVSGGNIDPKKTAAILSGMMP"
     gene            complement(6391..6981)
                     /locus_tag="VFM00_02490"
     CDS             complement(6391..6981)
                     /locus_tag="VFM00_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7497141.1"
                     /translation="MNRQLESRTPMQPGTSALALLVVSLVVLAIAIAIAPARSHAAEL
                     VPSYGLTRSVDGGDQVKGMFGLALRGNLIPNVLQSEIGAGYRTEDMSNGAIHVRQWPI
                     TASLLLTPMNVVYADAGVGWYHTTYDYESDLIGDETVQKFGVHLGGGIKVPVAPRVAL
                     DLGGRYVKLQDQESKLIPEKFDPSFWTLSMGLALKF"
     gene            complement(7440..7934)
                     /locus_tag="VFM00_02495"
     CDS             complement(7440..7934)
                     /locus_tag="VFM00_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497142.1"
                     /translation="MKTIHGIVLACFAVLATAGWTAPAQSFGLSGAGVRLGTTDAEDA
                     DASLSGGGHLEFEEAGSRVHIMPSVLFWSNSGLTDVNPNFDLYYHFAPAGRVSPYVGA
                     GAAVHMYSADGPGDPGTDLGLNLFGGAVFPMGSSRLFAELRYSATDLSQTSLFGGLTF
                     PISH"
     gene            8274..>8602
                     /locus_tag="VFM00_02500"
     CDS             8274..>8602
                     /locus_tag="VFM00_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497143.1"
                     /translation="MTRPWLASLVMALSVAGCGGPTPAEKTPIERAAFEDWKIEPGLR
                     IGALNRFGSELDLARAYGSRNLRDSSVALGEGETAPGTLLYPDDPQRRVEIIWSDPGA
                     KRAPRRAV"
BASE COUNT         1314 a         2939 c         2966 g         1383 t
ORIGIN      
        1 cgaggccctc ctcggtgcgc tccatgccgc agtagtccca gatgatccgg ccgagctcgc
       61 ggtggaagtg gtccggcgaa cgggtgccct tgatgttcag gtagccgcgg tagcgatccg
      121 ctgcctcccg ctcggcttcg gcgaacgccg ggtgatcggt gcctgggatc ggcttgttga
      181 gcatcggggc gagctcgttg ccgatcgtgt acggcaggac gaagtagccg tcggacaggc
      241 cctgcatgag cgctgacgcc ccgaggcggt tggcgccatg gtcggagaag ttggcctcgc
      301 ccagcacgtg gagccccggg atcgtggaca tcaagttgta gtccacccac aacccgccca
      361 tcgtgtagtg cgcggcgggg tagatccgca tggggacgtg gtaggggttc tcgtcggtga
      421 tccgctcgta catctcgaac agattcccgt atttttcccg gatcgccggc tccccgagcc
      481 gccggatcgc gtctccgaaa tcgagataga ccccgcgccc gccgggtccg accccccgcc
      541 cctcgtcgca gacctgcttc gccgcacgcg aggcgatgtc gcgcggggcg agattgccgt
      601 acgaggggta tttccgctcg agatagtagt cgcgctcctc ttcgggaatc agcgcgggcg
      661 cgcgcgcgtc cccctgctgc ttcggcaccc agacgcgtcc gtcgttccgg agcgactcgc
      721 tcatcagcgt gagcttggac tggtgctccc cggtgaccgg gatgcaggtc ggatggatct
      781 gagtgaagca ggggttcgcg aagccggcgc ccttcttgta cgcgcgccag atggcggtcg
      841 cgttcgcgcc cttggcgtag gtcgcgaggt agaagacgtt gccgtacccg ccggtggcca
      901 ggcagaccgc gtcggcgaga tgcgactcca cctggccggt cacgagatcg cgcgtgacga
      961 tgccgcgcgc gcggccgtcg atcaggatca gctccagcat ctcgtggcgc gcgaacatct
     1021 cgaccttgcc cagaccgacc tgacgctcga gcgcctgata ggcgccgagc aggagctgct
     1081 ggcccgtctg cccgcgcgcg tagaaggtgc gcgagacctg cgcgccgccg aaggagcggt
     1141 tcgccaggag gccgccgtac tcgcgcgcga agggcacccc ctgcgccacg cactgatcga
     1201 tgatgttcac cgagatctcg gccagccggt agacgttcgc ctcgcgcgag cggaagtcgc
     1261 cccccttgac cgtgtcgtag aacaggcgga agacgctgtc gccgtcgttc tggtagttct
     1321 tcgcggcgtt gatcccgccc tgcgccgcga tcgagtgcgc gcgccgcggg ctgtcctggt
     1381 agcagaagca cttcacgttg tagcccagct cggccaggct ggcggcgccc gaagcgcccg
     1441 cgaggccgct ccccaccatg atgaccgtgt acttccgctt gttcgccggg ttcaccagcc
     1501 ggatcgagga gcggtgcttc tcccacttcg tctccatcgg gccgggcgga accttcgcgt
     1561 cgagcttcat gcgcctaccg gatcacgccc gtcagcacgg cgagcgggaa ggagatgttc
     1621 cccacgacga cgatcgccgt catgagaccg gcgaatccgt ggcgcaggaa gttgtaccgc
     1681 ggatgcgaga ggcccagggt ctgcagcatg ctccagatgc cgtggtacag gtggtacccc
     1741 agggcgagca tcgcgacgat gtagaagatc gagacgggca cgacctgaaa gcccgcgacg
     1801 aagttgtgat agacgttccc ctccacgaac gaggggtgga ccgtgccgaa cgtgaagtgg
     1861 aggagatggt agatgatgaa gagcaggagg atcggaccgc tccagaccat cgtgcgcgac
     1921 gcgtaggtcg agcggtcgta ttcgcggcgg cgatagccga ccgggcgcgc gcggcggttg
     1981 tcgatcgtga gcgcggtcgc cgaccagatg tggaggatga ccgacaggag gagcaccgcc
     2041 cgggcgatcc agagccccgc gccatgcagg agctcgcgga gccataccgc gtaggcgttc
     2101 agagcctccg ggcccaggta ggcctggagg tttccgatca tgtgcgcgac gacgaagccg
     2161 aagagaatga agccggtgac cgccatgacc accttcttgc cgatggtcga tgcggcgaac
     2221 ggctccgctc ggctgaattg tccgggaccg acttgggttc gttccataga cacgatcagt
     2281 ggggcatctt agacctgccc ggccgggacg gcaagggcgg cgccggtgtt ctccggtata
     2341 cttttcggat gagccgaacc cgggccgcct ggatcctcgc catcgtcgcc gacacgcttc
     2401 agatcgtgct ccttcccgct ttcggcgagg gcctggcgtc gccggcggcg gacgcgctcg
     2461 acatcgtcgt cgcgctcggc atgaccgttc tcctcgggtg gcatttcgcg ttccttccca
     2521 ccgcgctcgc ggagatcgtc cccggcttga acctcgtacc cacgtggacg gcggccgtct
     2581 tcttcgtgac gcgaagccgt cgagcgcgcg ggagcgatat cccggaagag ccgggccgga
     2641 tgcaggagcc accccgaggg acgctgccgc cgccaccgca gtagggccgt caatctcggc
     2701 tgactcatgt cctgaatttc acccgcgcca gcgcgggtgc tttcttgttt cgatgccgcg
     2761 ccgacaggga ccggtgcgag taagcccata acgactccag gctggttgcg cccaccggag
     2821 tcgtcgccta atctcccgcg gtgaatcacc ggggagtagc ggcggcggtt tgcctgacgg
     2881 cctcactgtt cctgcacgta ccggcatggg gagccgaccc cgaaccggcg ccagtcgcca
     2941 cccgcggctc gagcccgcgc atcacggaga tcgcgatctt ccgggatgcc gcgatccgct
     3001 ggagtcccga ctccctgcgg ttcttcgcgc ggcgcggact ttcctacgtg gatgccgggc
     3061 gcggcgtcgt cacgacggtc gcgcttccca cgctgcgagg tccgcacagg atcacggcgg
     3121 ttctcacggt acacccggtt cccaaatcgg accgcgaggt ctgcgaccgc tacgatcgcg
     3181 cgggcagcat ccggatggcg gttccgggcg cgcccgacct cgaggttctc cgcttcatca
     3241 ccgcctacgg cggcagcacg aggcacgagg tggacgtcac cgccctggca ccgctcctcc
     3301 agggacggcg cacgttcacg gcccatatcg acacctggac cagccccgcc tggacggtgg
     3361 acgtctcgct ccgctacacg ccggtcgccg attacgaaga ccccacgtgg gcggcccccc
     3421 tgtatcgcac cgacaacttc aaccgcgaag cgatgccgga gggcgacagc gcgaccatcg
     3481 tgatccctcc ggggctcgcc cgcgtcgtgc tgcgctacac ggcgacgggc cactgcacgg
     3541 acggcgtgga cgccgacgag ttcgtctcga aagcgaacgt gatcgcggtg gacggcgtcg
     3601 tggtggcgcg cttccatccc tggcgcgacg actgccgctc cttccgggac cgcaatccct
     3661 actgcgcgcg gtggaccgac gggagatggt cctcggacta cgcgcgaagc ggatggtgcc
     3721 caggggtgga ggtgctcccc caggaattcg acctcaccga tcacctgcgc gccggccgtc
     3781 acacgatccg gatcgcgatc gaggacatgc gtcccaagga cgcgaaagga aactacggct
     3841 actggcgggt cgcggcagcg ctcgtgggct ggaagtcgcc gcctcgcctc tggcgcaacg
     3901 agtgacgtgc tgggtatcct cttccctgtg agcccgccac cccggcgcgc gcgcggcggc
     3961 gtccgcacga tcggccgcgc gctggcgcat cgaaactacc gcctcttctt caccggccag
     4021 agcatctccc tcatcggagc ctggctcacg cgggtggcga cgagctggct ggtctaccgg
     4081 ctcacccact cggcctggat gctgggcatc gtcggcttcg ccgggcaatt cccgacgttc
     4141 ctcctcgctc cgatcgcggg cgtctgggtg gaccgctgga accgccaccg cgtcctcgtc
     4201 gtcacgcagg tgctcgccgc ggtccagtcg gggctgctcg cctacctggc cctgcgcggc
     4261 atcatcacga ttccgcacgt ggtcgccctg agcatcctcc agggggtgat caacgccctg
     4321 gacatgccgg cgcggcaatc gttcgtggtc gacatgatcg gcaacaaccg cgaggacctg
     4381 ccgaacgcga tcgcgctcaa ctcctcgatg ttcaacggcg cgcgcctcat cggaccctcg
     4441 gtggcgggca ttctcatcgc ctgggtcggc gagggatggt gcttcttcat cgacgccgtg
     4501 agctacctgg cggtgatcgc gtcgctcttg atgatgaagg tcgcgttgtc ggagcgcccg
     4561 cggcgcgaaa gctcggtggt ccgcgagatc cgggagggct accgctacat cgcggggttc
     4621 accccgatcc gctccgtgct cttcctgctc gcgctggtca gcctgctcgg ctttccctac
     4681 accgtgctca tgccgatcat ggccgcgcag gtgctgcacg gcggaccgca cacgctcgga
     4741 tttctgatgg ccgcctcggg ggtgggcgcc ttgacggggg cgctctatct cgcttcgcgc
     4801 agctcggtgc tggggctggg gagcctgatc gcgcgaagcg ccgccggatt cggcgtgggt
     4861 ctcgtcctct tctccctctc gcggaacgtg tggctctcga tggcgctgat ggcgatctgc
     4921 ggcctcacga tgatccttca gatggccgcg agcaacacgg tgatccagac cctcgtggac
     4981 gacgacaagc ggggccgggt catgagcttc tactcgatgg cgttcttcgg aaccgcgccg
     5041 ctcgggagcc tcttcgccgg aacggtcgcc acctggatcg gcgtgccgcg cacgatcctc
     5101 gtgggcggtg cggcctgcgt gctcgcggcc ctcggattct ggaaagcgct cccccacatc
     5161 cggcgcgccg cccgccccgt ctacgtgcgg ctggggatcc tcccggcgcc gaccgagcag
     5221 agcctgtgac gcggcggacc cgccgcccgg tcgctccgtg agaacgctcg cactacccac
     5281 gctggatgaa gtccgcgcgg cgcgcgagcg gatccgcggc gtcgcgatcc gcacgccgct
     5341 cctccgcctg aacgtcgacg gcccggccga aatctggctc aagtgcgaga acctccagcc
     5401 gatcggctcc ttcaagctgc gcggtgccgg gaacgcgatg gcgctcctct cgcgcgaggc
     5461 gctcgagcgg ggcgtctata cggccagcgc gggcaacatg gcgcaggggg tcgcctggtg
     5521 cgcgaagcgc ctcggcattc cctgctccgt ggtggtgccc gatcacgcgc cggagatcaa
     5581 gacggcggcc atcgagcggc tgggcgcttc ggtcctcaag gttccgttcg cgtcttggtg
     5641 gcgcgtcatg acggagcatc gccacgaggg gatgaagggg accttcatcc atcccttctc
     5701 cgaccccgcc gtgctcgccg gcaacggaac gatcgccctg gaagtgctgg aggacctccc
     5761 cgaacccgac gccatcctgg ttccctacgg gggcggcgga ctgtcgaccg ggatcgccac
     5821 cgtcgcgcgc gcgctggcgc cgcgggcccg cgtccatgcg tgcgaggtcg ccacggccgc
     5881 cccgctcgcc gcgtccctgg ccgcgggcgc cccgaggacg gtgacctaca cacccagctt
     5941 cgtggacggg atcggcggca gcggcgtctt cgaggaggtc tggccccacg cgcgcgccct
     6001 gctcgcgggc agcctggtcg tctccttgga agaggcggcg tcggccctgc gcctcctcgt
     6061 ggagcgcaat cgcctggtgg ctgagggcgc gggcgcggtg ccggtggcgg cggcgctcag
     6121 gggcctggag ggcgcccgga cgatcgtctg cgtggtgtcg ggggggaaca tcgaccccaa
     6181 gaaaacggcc gcgatcctct cgggcatgat gccctgagcg gaccgcggcg taccgggccc
     6241 tggagatcgg cccgggccgg gcgcaggggg gaggaggcct cgtgtgcctc cccctccgta
     6301 ccctgccgcc tcttgtggat caccggcagc ggcagagcat gcgccctatc tatgtaaacg
     6361 tccgaccggc gcgaccgctc gggcggacga ctagaacttg agtgcgagac ccatcgacag
     6421 ggtccagaac gacggatcga acttctccgg aatcagcttc gactcctggt cctgcagctt
     6481 cacatagcgc ccgcccaggt ccaacgcgac ccgcggagcg accggcacct tgatgccgcc
     6541 gcccaggtgc acaccgaact tctggacggt ctcgtcgccg atcaggtcgc tctcgtagtc
     6601 atacgtcgtg tggtaccagc ccacgcccgc gtccgcgtac accacgttca tcggcgtcag
     6661 gaggagcgag gcggtgatcg gccactggcg cacgtggatg gcgccgttgg acatgtcttc
     6721 cgtccgatag cccgcgccga tctcgctctg cagcacgttc ggaatcaagt tgccgcgcag
     6781 agccagtccg aacattccct tcacctggtc gccaccgtcc accgagcggg tgaggccgta
     6841 cgaagggacg agctcggcgg catgcgagcg tgcgggagcg atcgcgatgg cgatcgcgag
     6901 caccaccagg gaaacgacga gaagcgcgag cgcggacgtg cccggctgca tcggcgtgcg
     6961 gctttcgagt tgtcggttca tcgcgctctc cttgggatcg atgtctctta tcggaatcgc
     7021 cgctctggcg gcgcgatctt cgaaactttg ggcgtccgcc cggctcggtc gggtcgaggc
     7081 aaggggggtg agaccgtggg gtccggccgg gcggcgcctg caccgggctt catcaaggcc
     7141 cgtgccacgg agggaacggg tcccggtccg cgggcgccaa cttggttaca gtgcaaaggt
     7201 tagagatttt gtggagccgc ctcggagcag cctgacgcga ccccgacgga attaccattt
     7261 ggaacatcgg ggaacggctc tgcacccgcc ggtgcagaaa cgaaaatccg gagggctggg
     7321 tgcgcgaggg tcgcggaagg ccgtaaagaa accaccggcg tccggtgggc acggcgaccg
     7381 cgggggcggg agccgcaccg tcgatccggt gcgccggtgg ccgaaacgac cagcctgtcc
     7441 tagtgcgaga tcgggaacgt cagccctccg aagagcgacg tctgcgacag gtccgtggcc
     7501 gagtagcgaa gctccgcgaa gagccggctc gaccccatcg gaaagacggc gcccccgaac
     7561 agattcagtc cgagatccgt gcccggatcg cccgggccgt cggcggaata catgtgcacc
     7621 gcggcgccgg cgcccacgta ggggctgacg cgccccgcgg gcgcaaagtg gtagtagaga
     7681 tcgaaattgg ggttcacgtc cgtcaggccg ctgttggacc agaagaggac gctgggcatg
     7741 atgtgcacgc gcgaccccgc ctcttcgaac tcgagatggc cgccgccgga cagcgacgcg
     7801 tcggcgtcct cggcgtcggt ggtgccgagg cggacgcccg ctccggaaag gccgaacgac
     7861 tgcgcgggcg cggtccaccc ggcggtcgcg agcaccgcaa agcatgccag gacgatcccg
     7921 tggattgtct tcatggttcc tccctctggg gttttgagga gccgttggcc actggaggcg
     7981 aacgactgcc ggcggcgagg aatctcaagg gccatgccag agggaacgcc aaggccgccc
     8041 ggccgcgggt agcgcatgac cgcggtgcgg attaggacaa ggcgggtgga ggggtcgccg
     8101 cggaggcgat gtcgcgcgac agccgaccgg ggccttgaag aatcagccta ccggcgggaa
     8161 cgaatgacaa ccgcgaccgt caagccgctg tcggaggcgt cccccggtaa gggccgctcc
     8221 ttgacaccct cccgatccgg cgggagcatg acccgcaaga aaggaggtct gtcgtgacgc
     8281 ggccgtggtt ggcttccctc gtgatggcgc tttccgtcgc cggctgcggc gggccaacgc
     8341 ccgccgagaa gacgccgatc gaacgcgccg ccttcgagga ctggaagatc gagcccgggc
     8401 ttcggatcgg cgcgctcaac cgcttcggct ccgagctgga cctggcgcgg gcctacggct
     8461 cccgaaacct ccgcgattcc tcggtcgccc tcggcgaagg ggagacggcg cccggcacgc
     8521 tgctctaccc ggacgaccca cagcgccggg tcgagatcat ctggtccgat cccggcgcca
     8581 agagggcgcc tcggcgcgcc gt
//
LOCUS       DASSLZ010000069         5438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_785759, whole genome shotgun sequence.
ACCESSION   DASSLZ010000069 DASSLZ010000000
VERSION     DASSLZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785759"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1363)
                     /locus_tag="VFM00_02505"
     CDS             complement(80..1363)
                     /locus_tag="VFM00_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HET7497144.1"
                     /translation="MDEFLVMGRRLSLPSFVMTLVSTWYGGVLGVGEYSYRYGISNWL
                     VFGAPYYVGAALFALFIARRARASQLYSVPDQLESAYGRASALIGAVVIQFLSSPAPY
                     VLMVGILAQLIFGGPLWVCIVGGTAISTAYSLRGGLRSVVRADNLQFILMYAGFLVAL
                     PLLVARYGGLGFLTTHLPHTHFVWHGGNAPQYVFVWYLIALQTLVEPTFYQRAFAAQD
                     EKTAVRGVWISIGFWILFDFLTTFTGMYARALMPHLADPVSSYPELAIRFLPPVVRGL
                     FYLGLFATVMSTVDGYTFIGGVNFGRDLVWRWRRERDESRVNGYVQIGFVITAAVSIA
                     LALFFRSAVDLWHDVGSVGVSALLVPLVTSYRAGWRTRPAFAAASMTVGGAVALAWLL
                     AARLSGGAYPLGIEPIYPGLLASAILWAGGRIVRS"
     gene            complement(1456..4065)
                     /locus_tag="VFM00_02510"
     CDS             complement(1456..4065)
                     /locus_tag="VFM00_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7497145.1"
                     /translation="MSPLRFAGRLLSGAAVALVLFAGASAVRAQTDSSAPPVYPRILR
                     GQVVDAERRPVADARVRTVDAPSGGARETATDANGEFQLTGLAAPRGTVEVSRLGYST
                     TRVAYDVTDPNAGALIVRLEAHALPLPTIEVSTTRAVERESPVPFTELPKPEIQARYW
                     AQDPPMLLAETPGVYAYSDAGNGVGYSYVKIRGFAQRRVAVTIDGIPLNDPESHEVYW
                     VDHPDLLSGTASVQVQRGVGSALYGASAVGGSMNVELLQVPRERRIAFEAGGGSYGTT
                     RFTALYQSGLLDNTYAISGRYSRVVSDGYREQSWSRLWAYQFTAARLDSWMTTRVNLY
                     GGPEQLHLAYYAVDRSYLDGRYTGDPDRDRRFNPLNWRNETDNFFEPHYELINDIRLT
                     DRASLTSSAFYFPGKGYYDDLPYGPQSFASRQLPSFYVPSDTLYPAAYYLTDSLGVPE
                     AQPNGQFLVTGSDMTQRLWVRNRHYGWVPRARLKHEKGELTLGGEWREHDGRHWGELT
                     WSAALPPGTEPNHVFYDYTGRVRVLSGFAQEAYNLRHDVRLTGSLQLRSTRYAIGDDR
                     YNGYDFHLNYSFLNPRVGVNWNPNERWNAFASFAYVRVEPILSEIYRADDPTSVPLFR
                     RLDPANHIYEDPLIDPEHLRDFEAGLGWKQGNAFLRLTGFWLDFRNEIVPFGQIDVLG
                     NPITGNAARSAHKGVEAELGYTHRSGMELSGNATFGKNRFGDYREFIAPGVFNDYSGN
                     AIAGFPDRMANVTVGYRRGGSRIAATMVETGRQYLDNSEDNRRNSALRAVQGYQHKFV
                     EEHAVLNAALTLDLTPLVGARALGQKSLALDVHGMNLTGLRYETAGYVFDEIPYFYPA
                     SKRSVFAALRVEF"
     regulatory      complement(4131..4225)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4255..5238)
                     /locus_tag="VFM00_02515"
     CDS             complement(4255..5238)
                     /locus_tag="VFM00_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497146.1"
                     /translation="MRSSPRTRKSISFVPILLLVGLTSCGRDTPTAPLLGNQASLSGR
                     ASLAAAPQGAFFPLAIGNRWHSVGDDRFVMTDAGGGVDGFTIHDDITRELTGTEVLFG
                     RTYTVMKETTVQTIPFESEPWISTQWIRYRQDASGLYEADVSGASEPVMSSAATGPDG
                     VRMQAARPLPDALRAKILPDRIDAYERAWNRLQWKVEVLNRYVASDGAARGSGVLPNE
                     ITRLVYPLRPGEEWTIRTDFFFGSVVEGIEDLDLPAGRFPAYRIRIPNEALGPEDVVR
                     LWMGRSGQLRFQYHLEAPATDEDGNVIGSSSIDHDEVLDQISLVKPSSLTP"
BASE COUNT          963 a         1776 c         1866 g          833 t
ORIGIN      
        1 aacgagtgaa gtagacggta cctgaccaac tgcgccctag aggggtcgca gcgaattggg
       61 gacaggcggc acgcatcgac tagcttctta cgattcgccc acccgcccat agtatcgcgc
      121 tcgccaacag gccggggtag atcggttcga tcccgagcgg gtaggcgccg ccgctcaggc
      181 gggccgcgag gagccacgcg agcgccaccg ctcccccgac ggtcatcgag gccgcggcga
      241 acgcgggccg ggtgcgccag cccgcccggt aggacgtgac cagggggacg agcagcgccg
      301 aaacgccgac ggagccgacg tcgtgccaca ggtccacggc ggagcgaaag aagagggcca
      361 gcgcgatcga caccgcggcc gtgatcacga atccgatctg cacgtatccg ttcacgcgcg
      421 actcgtcgcg ctcccgccgc cagcgccaga ccaggtcgcg cccgaaattc acgccgccga
      481 tgaacgtgta gccgtccacc gtggacatca ccgtcgcgaa cagcccgagg tagaagaggc
      541 cccggaccac ggggggcagg aagcggatgg ccagctcggg ataggacgaa accggatcgg
      601 ccaggtgcgg catcagcgcc cgcgcgtaca tgccggtgaa cgtcgtgagg aagtcgaaga
      661 ggatccagaa gccgatcgag atccagacgc cgcgcacggc ggtcttctcg tcctgcgccg
      721 cgaaggcgcg ttggtagaag gttggctcga ccaatgtctg cagcgcgatc aggtaccaga
      781 cgaacacgta ctgcggcgcg ttcccgccgt gccagacgaa gtgcgtgtgc gggaggtgcg
      841 tggtcaggaa gccgagtccg ccatagcgcg cgaccaggag cggcagcgcc accaggaacc
      901 ccgcgtacat gaggatgaac tggaggttgt cggcgcgcac cacggagcga agccctccgc
      961 gcagcgagta cgcggtggag atcgcggtgc cgcccacgat gcagacccag agcggcccgc
     1021 cgaagatgag ctgggccagg atgcccacca tcagcacgta gggcgcgggc gaagagagga
     1081 actggatcac cacggcgccg atcagcgcgg aggcgcgccc gtaggcggac tccagctggt
     1141 ccgggaccga gtagagctgg gaagcgcggg cgcggcgggc gatgaacagc gcgaagagtg
     1201 ccgcgccgac gtaatagggc gcgccgaaca cgagccagtt ggagatgccg tagcggtagg
     1261 agtactcccc cacgccgagc acgccgccat accaggtcga gaccagcgtc atgacgaacg
     1321 acggcaagga gagccggcgc cccatgacca ggaactcgtc caccggcttc ttcgccgtgc
     1381 cgaagagccg gaatccgaga tagagcagga acccggcgta gagcgcgagg agtacgaggt
     1441 cgacgggcgc cgtgcctaga actccacgcg gagggccgcg aacacgctcc gcttcgaggc
     1501 cgggtagaag taggggatct cgtcgaagac atatcccgcc gtctcgtagc gaagcccggt
     1561 cagattcatc ccgtgcacgt ccagcgcgag cgacttctgc cccagcgcgc gcgccccgac
     1621 cagcggcgtc aggtccagcg tcagggcggc gttcagaacc gcgtgctcct cgacgaactt
     1681 gtgctgatat ccctgtaccg cgcgaagcgc ggaattcctt cgattgtcct cggaattgtc
     1741 gagatactgc ctacccgttt cgaccatggt ggctgcgatt ctagaaccac cgcgccgata
     1801 gcccaccgtc acattcgcca tccggtcggg aaatcccgcg atggcgttcc cgctgtagtc
     1861 gttgaagacc cccggtgcga tgaactcccg gtagtcgccg aagcggttct tgccgaatgt
     1921 ggcgttgccc gagagctcca tcccgctccg gtgcgtatag ccgagctcgg cttcgacccc
     1981 cttgtgcgcc gagcgcgccg cgtttccggt gatcggattg ccgaggacgt cgatctggcc
     2041 gaagggcacg atctcgttcc ggaaatccag ccagaatccg gtcaggcgca ggaaggcgtt
     2101 tccctgcttc cagccgagcc cggcttcgaa atcgcgcagg tgctcggggt cgatcagggg
     2161 gtcttcgtag atgtggttcg ccggatccag ccggcggaag agcggcacgc tggtcgggtc
     2221 gtcggcgcgg tagatctcgc tcaatatcgg ctccacgcgc acgtaggcga aggacgcgaa
     2281 cgcgttccac cgctcatttg gattccagtt cacgcccacg cgcgggttca ggaacgagta
     2341 gttcaggtgg aaatcgtagc cgttgtaccg gtcgtcgccg atggcatagc gggtgctcct
     2401 cagctgaagg ctgccggtca ggcgcacgtc gtggcgcaga ttgtaggctt cctgcgcgaa
     2461 gcccgacagg acgcgcacgc ggccggtgta gtcgtagaac acgtggttcg gctcagtccc
     2521 cggcggtagc gccgcgctcc aggtcagctc gccccagtgg cggccgtcgt gctcgcgcca
     2581 ctcgccgccc agggtcagct cgcccttctc atgcttcagg cgcgcgcgcg gcacccatcc
     2641 gtagtgccgg ttccgcaccc acagccgctg cgtcatgtcc gagccggtca ccaggaactg
     2701 gccgttcggt tgcgcttccg gcactccgag cgaatcggtc aggtagtacg cggccggata
     2761 cagcgtgtcc gagggaacgt agaaggatgg gagctgccgc gacgcgaacg actgcgggcc
     2821 gtacggcagg tcgtcgtagt agcccttgcc cgggaagtag aaggccgacg acgtgagcga
     2881 cgcgcgatcg gtcaggcgga tgtcgttgat cagctcgtag tgcggctcga agaagttgtc
     2941 agtctcgttc cgccagttga gcgggttgaa gcggcggtcg cgatcgggat cgcccgtata
     3001 tcggccatcc aggtacgagc ggtccacggc gtagtaggcc aggtggagct gctccggtcc
     3061 gccgtacaga ttgacgcgcg tcgtcatcca ggagtcgagg cgcgccgccg tgaactgata
     3121 ggcccagagc cgcgaccagg actgctcgcg gtagccgtcg ctcacaacgc gggagtagcg
     3181 tcccgagatg gcgtaggtgt tgtcgagcag cccgctctgg tagagcgcgg tgaagcgcgt
     3241 cgtgccgtac gagccgccgc ccgcttcgaa cgcgatccgc cgttcgcgcg gcacctgcaa
     3301 cagctcgacg ttcatagagc cgcccacggc gctcgccccg tagagcgcgc ttcccacgcc
     3361 gcgctgcacc tgcaccgatg ccgtccccga gaggagatcg gggtggtcca cccagtagac
     3421 ctcgtgcgat tcggggtcgt tcagcgggat gccgtcgatc gtcacggcca cgcgccgctg
     3481 cgcaaatccc cgaatcttga cgtacgaata gcccacgccg ttccccgcgt cggagtaggc
     3541 gtagacgccg ggcgtctcgg ccaggagcat cggcgggtcc tgcgcccagt agcgcgcctg
     3601 gatttccggc ttcggcaact cggtgaaggg cacgggggat tcgcgctcga cggcgcgcgt
     3661 cgtggagacc tcgatcgtgg ggagcgggag cgcgtgcgcc tcgagccgaa cgatcagtgc
     3721 gcccgcgttc ggatcggtca cgtcgtaggc gacgcgcgtc gtggagtagc cgagccgcga
     3781 cacctcgacc gtgccgcgcg gcgccgccag gccggtgagc tggaactcgc cgttggcgtc
     3841 ggtcgcggtt tcgcgcgcgc ctccgctcgg cgcgtccacc gtgcggacgc gcgcgtcggc
     3901 gacgggacga cgctcggcgt ccaccacctg cccgcgcagg atgcgcgggt acacgggggg
     3961 agcgctcgaa tcggtctgag cgcggacggc cgaggcgccg gcgaagagga cgagcgctac
     4021 agcggcaccg gagagcagcc ggccggcgaa gcggagaggc gacatcgagg gcaccttcct
     4081 tcccaagcaa tcgggccgcg cccaggagag gacgcggccc gacgagaagg tctcttgtcg
     4141 tcccgtttcc ctacgctggt atgacccaga tcaggttcta gggtttgatc tcaggccggt
     4201 atcccggcca cccctaacgg acggtgtcac gatgcgcccc cggaacgggg gttgtcaagg
     4261 cgtgagcgat gagggcttca ccaggctgat ctggtccagg acctcgtcgt ggtcgatgct
     4321 ggagctcccg atcacgttgc catcctcgtc cgtggccggc gcctccaggt ggtactggaa
     4381 gcggagctgg ccggaccggc ccatccacag acggacgacg tcctcaggtc caagggcttc
     4441 gttcggaatc cggatccggt acgccgggaa tcgccctgcc ggaaggtcca ggtcttcgat
     4501 cccttccaca acggaaccga agaagaaatc ggtgcgaatg gtccactcct ccccgggacg
     4561 cagcggatac acgaggcgcg tgatttcgtt gggcagaacg cccgaaccac gcgctgctcc
     4621 gtccgaagcc acgtagcgat tcagcacctc gaccttccat tgaaggcgat tccaggcgcg
     4681 ctcgtaggca tcgatccggt ccggcaggat cttggcgcgc agcgcatcgg gcagcggacg
     4741 agctgcctgc atgcgcacgc cgtcgggccc ggtcgcggcg gacgacatca ccggctccga
     4801 cgccccggac acatccgctt cataaaggcc ggatgcgtcc tgacggtatc ggatccactg
     4861 cgtgctgatc cacggctcgg actcgaacgg tatggtctga accgtggtct ccttcatgac
     4921 cgtatacgtt cgaccgaaga gaacctccgt gccggtcaac tcgcgcgtga tgtcatcgtg
     4981 aatcgtgaag ccgtcgacgc cgccgccggc gtccgtcatg acgaaccggt catcgccgac
     5041 cgagtgccaa cggttgccga tggcgagtgg aaagaaggcc ccctgtgggg cggccgccag
     5101 cgaagcgcgg ccgctcaggg atgcctggtt acccaggagt ggagcggtgg gcgtatcgcg
     5161 gccgcagctt gtaaggccca ccaggagaag gatcggaacg aagctgatgc tcttgcgggt
     5221 ccgaggactg gagcgcatgg gtccacctcg cgatgcggct tcgaaatgcg ggttgcaagg
     5281 actcattagc ataagggacc gtggggatct gccgataggc tactcatgac caccccggct
     5341 gccattctga catgcgccct tctcgccgcc gccccgttgt cggcgcccca tcccgcgggc
     5401 tcgacccagg tcattaccga tgtgcagggg cggctctg
//
LOCUS       DASSLZ010000070         3899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1040316, whole genome shotgun sequence.
ACCESSION   DASSLZ010000070 DASSLZ010000000
VERSION     DASSLZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040316"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VFM00_02520"
     CDS             complement(<1..774)
                     /locus_tag="VFM00_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7497147.1"
                     /translation="MTRAPAVPGLAERDPSLQLRELSSGFIAPRCLYVVAELGVADAL
                     NDHPEGVDALARTVGADPDALRRVLRLLAAYGVFAIEGDQVRQTPLSRMLREDHPQSM
                     RPLVRLSGLPINWAAYGALLDTVRHGRPDEVPSHRWVYYAEHPGEAAIFNAAMVSKAR
                     RQVAAVAAAYDFSRFPTIGDIGGGHGHLVRTILESTPKARGVVFDLPHVIEDARRAVT
                     DERVDFRAGSFFDDALPTCDLYLLMEVVHDWPDPEAAAIL"
     gene            complement(806..1462)
                     /locus_tag="VFM00_02525"
     CDS             complement(806..1462)
                     /locus_tag="VFM00_02525"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7497148.1"
                     /translation="MSALRGLDPKELAPADVYALLTGCVVPRPIAFVSSLSAGGVANL
                     APFSFFNAGGGNPPSLIFSPVTSGGLRDKDTLHNVRATREYVVHIAPWGVRERMNQTS
                     AEYPPEVDEFEAAGFTKVASTIVKPWRAAECPIAMECRLFQIVEHGQGPYHANYVIGE
                     VVYFHIQESLMRGQRVDSAALDAIGRLGGPNYTRVTPESIFAMPRPATPESPAAGTKP
                     "
     gene            complement(1459..2649)
                     /locus_tag="VFM00_02530"
     CDS             complement(1459..2649)
                     /locus_tag="VFM00_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016132229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HET7497149.1"
                     /translation="MPMYHTLGQIPHKRHTQFRRPDGKLYTEQLMGSRGFSGRSSLIY
                     HHNMPTQAKEIKKVQDCSVGYAAEQGLRHYHFKTKGLHKQGDPVSGRVVLLHNADVSM
                     AIAVPERTMDYWYRNGQGDDLYFIHEGTGRIQTIFGELHYGSGDYVVIPRGTTYRFEP
                     SADEQRYLVIESASSIETPRRYRNEYGQLLEHAPMSERDIRKPERLETFTESGRYEVR
                     IKARNQITAYEFEFHPLDVVGWDGFLYPYIFNIGDFEPITGRIHMPPPTHQCFEGRNF
                     VVCSFVPRMYDYHPLAVPVPYNHSNVDSDEVLYYVNGNFMSRRGIEVGSFTLHPSGIP
                     HGPHPGATEASLGAKETKELAVMLDTFHPLNVTTEAEPYDDKGYPYSWLEPSGKFTGE
                     GATG"
     gene            complement(2672..3655)
                     /locus_tag="VFM00_02535"
     CDS             complement(2672..3655)
                     /locus_tag="VFM00_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7497150.1"
                     /translation="MRLVSFARPGAPGRALGVHEEDGILHLAHAAAILEIPEKDRALL
                     ATMESLLAEWDRGEPLAREVAVQARAAGDRAAAFLSAVRFRPEHVALLPPVAQPPTVR
                     DFYAFETHVKNARARRGLTVPPEWYEAPVFYFSNPGALIGDGAPVARPASSKALDYEL
                     EIACVIGRRARDVAADDWRSVVAGFTILNDWSARDVQRKEMAVGLGPAKGKDFATSLG
                     PVLVTLDALADRMSAAPGAGDRLDLAMEARVNGKTLSRANAKEMQFTFGQMIARASAD
                     VYLFPGDVIGSGTAGNGCLLELGPETHPWLEPGDTVTLEIERLGTLTNRIA"
     gene            complement(3675..>3899)
                     /locus_tag="VFM00_02540"
     CDS             complement(3675..>3899)
                     /locus_tag="VFM00_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009054081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HET7497151.1"
                     /translation="GSIDENLDALAALGILVDRDEEGYLLQIFSKPVQDRPTVFYEVI
                     QRKGSRGFGKGNFKALFEAIEREQALRGNL"
BASE COUNT          617 a         1305 c         1353 g          624 t
ORIGIN      
        1 gagaatggcg gcggcttccg gatcgggcca gtcgtggacg acctccatca ggagatacag
       61 atcgcacgtc ggaagagcgt cgtcgaagaa cgacccggcg cgaaaatcca cgcgctcgtc
      121 ggtgacggcc cgccgcgcgt cttcgatcac gtgcgggagg tcgaaaacga cgcccctggc
      181 cttgggcgtg gattccagga tggtgcgcac gaggtggccg tgtcccccgc cgatgtcgcc
      241 gatcgtcggg aagcgcgaga agtcgtaggc ggcggccacc gcggcgacct gcctccgtgc
      301 cttggagacc atcgctgcgt tgaagatcgc cgcttcgccc ggatgctccg cgtaatagac
      361 ccagcggtgg ctggggactt cgtccggccg tccatgccgc acggtgtcca ggagtgcgcc
      421 gtaggccgcc cagttgatcg gaaggccgga caaccggacg agcggccgca tcgactgcgg
      481 gtggtcctcg cgcagcattc gggagagggg ggtttgccgg acctgatccc cttcgatcgc
      541 gaagaccccg taggcggcga ggagccgcag cacgcgccgg agggcgtcgg gatcggcacc
      601 gaccgttcgc gccagcgcgt cgacgccctc ggggtggtcg ttcagcgcgt ccgccacgcc
      661 aagctcggcg acgacgtaga gacagcgcgg agcgatgaat cccgacgaaa gctcgcgcaa
      721 ctgaagcgac gggtcgcgct ccgcgagtcc cggcaccgcc ggagcacgcg tcatggccca
      781 gcacccgacg cgggcacttc gcgcgtcacg gcttcgtacc cgccgccggc gattccggcg
      841 tcgcgggacg cggcatcgcg aagatcgact ccggagtcac gcgcgtgtag ttgggaccac
      901 ccaggcggcc gatggcgtcg agcgcggcgg aatcgacccg ctgccccctc atcagcgatt
      961 cctggatgtg gaagtagacc acttcgccga tcacgtagtt cgcgtggtag gggccctggc
     1021 cgtgctcgac gatctggaac aggcggcatt ccatggcgat cggacactcg gcggcgcgcc
     1081 acggcttgac gatcgtgctc gcgaccttcg tgaacccggc ggcctcgaac tcgtccacct
     1141 cgggtggata ctcggccgag gtctggttca tccgctcccg cacgccccag ggagcgatgt
     1201 gcaccacgta ctcgcgggtc gcgcgcacgt tgtgcagcgt gtccttgtcg cgaaggccgc
     1261 cgctcgtgac cggggagaag atcaaggaag gcggatttcc gccgccggcg ttgaagaagg
     1321 agaacggcgc cagattcgcg acaccgcccg cgctcaggct ggagacgaac gcgatcgggc
     1381 gaggcacgac gcagcccgtg agcagcgcat agacgtccgc gggggccagc tccttcgggt
     1441 cgagcccccg gagggcgctc aaccggtcgc gccctcgccg gtgaacttgc cggacggttc
     1501 gagccacgaa tagggatagc ccttgtcgtc gtacggctca gcctcggtcg tcacgttcag
     1561 cgggtggaac gtgtcgagca tgaccgcgag ctccttcgtc tccttggccc ccagcgaggc
     1621 ctccgtggcg cccggatgcg gtccgtgcgg aattcccgaa gggtgcagcg tgaaggagcc
     1681 gacctcgatg ccgcgccggc tcatgaagtt cccgttcacg tagtagagca cctcgtcgga
     1741 atccacgttc gagtggttgt acggcaccgg caccgcgagc ggatggtagt cgtacatgcg
     1801 cgggacgaac gagcagacca cgaagtttcg cccctcaaag cactggtgcg tgggcggcgg
     1861 catgtggatg cggccggtga tcggctcgaa gtcgccgatg ttgaagatgt agggatagag
     1921 gaagccgtcc cagcccacca cgtcgagcgg atggaactcg aactcgtacg ccgtgatctg
     1981 gttccgggcc ttgatccgca cttcgtaccg cccgctctcg gtgaaagtct ccagccgctc
     2041 cggcttccgg atgtcgcgct cgctcatggg cgcgtgctcg agcagctggc cgtattcgtt
     2101 gcggtaacgg cgcggcgtct cgatggagga ggcggactcg atcaccagat agcgctgctc
     2161 gtcggcgctg ggctcgaacc ggtaggtggt cccgcgcggg atcacgacgt agtcgcccga
     2221 cccgtagtgc agctcgccga agatcgtctg gatgcggccc gtgccctcgt ggatgaagta
     2281 gagatcgtcc ccctggccgt tccggtacca gtaatccatc gtccgctcgg gcacggcgat
     2341 cgccatcgaa acgtcggcgt tgtggaggag cacaacccgt ccggagaccg ggtcgccctg
     2401 cttgtgcagg cccttcgtct tgaagtggta gtggcgcagc ccctgctccg cggcatagcc
     2461 caccgagcag tcctggacct tcttgatctc cttggcctgc gtcggcatgt tgtggtggta
     2521 gatcagcgag gagcgcccgg aaaagccccg cgagcccatg agctgctcgg tgtagagctt
     2581 cccgtccgga cgccggaact gggtgtgccg cttgtgcggg atctgaccca gggtgtggta
     2641 catcggcatg gcgtttcctc tcgaatcgag atcaggcgat gcggttggtg agggtcccga
     2701 gacgctcgat ctcgagggtc accgtgtcgc caggttccag ccaggggtgc gtttcgggac
     2761 ccaactcgag gaggcagccg ttcccggccg tacccgaccc gatcacgtcg cccggaaaaa
     2821 gatacacgtc cgccgaagcc ctcgcaatca tctggccgaa ggtgaactgc atctccttgg
     2881 cgttggcgcg cgacagcgtc ttgccgttca cgcgcgcctc catcgccagg tccagtcggt
     2941 ccccggcgcc gggcgcggcg ctcatccgat ccgcgagcgc gtccagcgtc acgagcaccg
     3001 ggccgagcga cgtggcgaaa tcctttccct tcgccggtcc gagacccacg gccatctcct
     3061 ttcgctggac gtcccgcgcg ctccagtcgt tcaggatcgt gaaccccgcc accacggatc
     3121 gccagtcgtc cgccgccacg tcgcgggcgc gccggccgat cacgcaggcg atctccagct
     3181 cgtagtccag cgccttcgac gaggcgggcc gcgcgaccgg cgccccatcg ccgatcaacg
     3241 cccccgggtt cgagaagtag aacaccgggg cttcgtacca ttccggtgga accgtgaggc
     3301 cgcgccgggc gcgggcgttc ttcacgtggg tctcgaacgc gtagaagtcg cgcacggtgg
     3361 ggggctgcgc cacgggagga agcagggcga cgtgctcggg gcggaaccgg accgcgctca
     3421 ggaaggcggc cgcgcggtcc cccgcggcgc gagcctggac ggcgacctcg cgcgcgagcg
     3481 gctcgccgcg atcccactcc gcgaggagcg actccatcgt ggcgaggagg gcgcgatcct
     3541 tctccggaat ctcgaggatg gccgcggcgt gggccagatg gaggatcccg tcctcttcgt
     3601 ggacgcccag cgcgcggccg ggcgcgccgg ggcgggcgaa ggagacgagc cgcatccggt
     3661 cgaggagccg gggcctacag gttcccgcgg agcgcctgct cgcgctcgat cgcttcgaac
     3721 agggccttga agttcccctt gccgaagccg cggcttccct tgcgctggat cacctcgtag
     3781 aagacggtcg ggcggtcctg caccggcttg gagaagatct ggagcaggta gccctcttcg
     3841 tcgcggtcta ccaggatgcc gagcgccgcc agcgcatcca ggttctcgtc gatcgaccc
//
LOCUS       DASSLZ010000071         7398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1547998, whole genome shotgun sequence.
ACCESSION   DASSLZ010000071 DASSLZ010000000
VERSION     DASSLZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547998"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..485)
                     /locus_tag="VFM00_02545"
     CDS             complement(81..485)
                     /locus_tag="VFM00_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017138254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET7497152.1"
                     /translation="MRRILALALMVGLVPGCAGSRSIPIAVAPSGSEERLAFKEEGYA
                     SYYGREHHGRRTASGERFDAGRLTAAHRTLPFGTRVRVTNLDNGRKVVVTVTDRGPFR
                     RHRVIDVSRRAAEDLGFLREGTARVRLEVLSG"
     gene            820..3129
                     /locus_tag="VFM00_02550"
     CDS             820..3129
                     /locus_tag="VFM00_02550"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HET7497153.1"
                     /translation="MADALYKEALEYHRGARPGKIEVVSTKPCATQKDLSLAYTPGVA
                     VPCLEIEKNPDLAYEYTARGNLVAVISNGTAVLGLGDIGPLAGKPVMEGKGVLFKRFA
                     DVDVFDIEIASKDPEEIVRLCALLEPTFGGINLEDIKAPECFTIEQKLQSLVSIPVFH
                     DDQHGTAIITGAALLNALEVTGKKLTDIRVVILGAGASGIASARHFTKLGLRQDQIMM
                     CDRKGVVHTGRLAELNPHKAEFAVETTARTVADALRGADVLLGLSSAGLVTQEMVRSM
                     ASKPILFACANPDPEISYPDAKAARPDVLMATGRSDYPNQVNNVLGFPFLFRGALDVR
                     ARRFNDEMKLAATRALADLAKQDVPDAVARAYGVETLHFGPDYLIPKPFDPRVLLWVA
                     PAVAKAAMETGVARLTIDLDEYRDRLEARLGKSREIARAVMHRARRSPRRIVFPEGTH
                     PRILRVATHLMAEGVARPVLLGKPEEIEREARHLDLSLAGIERVHPRRHPKREAYVDA
                     LYRMRQRKGLTAKEADTLLLNPNYFGTMMVHLGDADGLVSGVTQHYPDTIRPALQIIG
                     LRPGITRVCGVYCIVARRKCWFFADTTVNIQPTAEELAEIAILAAGVAREFGFDPRVA
                     MLSFSSFGSAKHPLVDTVREAARLVAKRAPDLVVDGEMQLEAAVEPTVAHEHFPFSRI
                     QGDANVLVFPDLQSGNLAYKLMQRLGGAETIGPILTGLAKPIHIVSPTSDDNEIIHIA
                     SIAVLEAQLRDEERERAARRPAIPVEELV"
     gene            3129..4262
                     /locus_tag="VFM00_02555"
     CDS             3129..4262
                     /locus_tag="VFM00_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET7497154.1"
                     /translation="MDIGILKETYSGETRVALIPFAVGELVRQGNRVVIQSDAGASSG
                     FDDQAYRDAGATIVYSAEEVIGRSRVILKVIPPSLEECRRLEEGKILFSFLHLSSAPR
                     SLVEHLVRRRVTAIGFEAIGDENGDRPVLTAMSEIAGKLAIPIAAQYLQTRQGGRGIM
                     LDGIPGVPPGIVVILGAGVVGSTAAVRAAATGAQVIVLDRDVERLRRLHARVPAFVTT
                     AVATPYNIERFVPIADALIGAVLIPMETTPHVLDERLVRSMRPRSVFLDVSIDQGGCS
                     TTSRPTNILDPTYVREGVVHFCVPNIPALVPRTATAALSNIILPYLTEIARLGPDRVL
                     HDDPVLARGVYTHDGYCLEPKIASVLDLPYRGAERERSYDVAR"
     gene            4249..5592
                     /locus_tag="VFM00_02560"
     CDS             4249..5592
                     /locus_tag="VFM00_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014629242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HET7497155.1"
                     /translation="MSPASLNAPAVATPTPPRTTLDYHDKLATPEEALRLVRPGDTVY
                     VHPGCAAPQTLLAALVARKDDLHDVRVIHLMSQGRADYALPGMEPHFRHVALFIGKNT
                     RDAVNDGRADYLPVFLSEIPRLFTTRTVPLEVALIHVSPPDEHGFCSFGVGVECTKAA
                     ASTARVVIAQVNPRMPRVLGDNFIHVRNVTRFVEVDEPLVELPRVRMADAFDEIGRNV
                     AALVEDGSTLQLGIGGIPDAVLHHLRDRKHLGVHTEMFSDGMVELVQAGIVTSERKTL
                     HPGKIVASFVLGTQPLFDFIHDNPIVEFHPSDYVNDPFVIAQNDRMVAINSAIQIDLT
                     GQVCSDSIGDMAYSGFGGQVDFVRGASRARGGKAIIALPSTARGGEVSRIAPYLDPGA
                     GVVTSRADVHFVATEFGVADLYGKSLRERARALIDIAHPRFREELERGAMKRKLL"
     gene            5628..6758
                     /gene="asnA"
                     /locus_tag="VFM00_02565"
     CDS             5628..6758
                     /gene="asnA"
                     /locus_tag="VFM00_02565"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET7497156.1"
                     /translation="MASTKTADLAGPGISTYEEVERILPNGYRSLLDPKETQLAIHAL
                     KLHLEENLCKALSLFHVEVPLVVTRESGLNDYLDRDGSRTPVEFRCGLGLEERMHTQV
                     VQAATKWKRLALRQFECVPGEGICTDMRAVRKDYFLDHDHSAYVDQWDWEKAIRPEQR
                     NLTYLKETVRAIWGVIHGAEVFIRKEFPKLQASPFPPLPEELTFLHAEDLLDMYPDLP
                     RKQRETAILQKHPAVFIIGIGWVLKDGYPHEMRAADYDDWVTPTVSEDGRPMHGLNGD
                     ILVWNPVTQRRHELTSMGIRVTKETLRQQLEITGQLDFLKLPYHQAILNDEIPLSIGG
                     GIGQSRTVMLLLRKAHLGEVSVTVWPDELNRICERKNIHVLQ"
     gene            6821..>7398
                     /locus_tag="VFM00_02570"
     CDS             6821..>7398
                     /locus_tag="VFM00_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HET7497157.1"
                     /translation="MSGLDVAVRTPGKSMTAKEYVQNVMAGIKAKNPAEPEFHQASQE
                     VFESLVPMLERHPEYRAAKLLERIAEPERVLMFRVPWQDDRGEVQVNRGFRIEMNSAI
                     GPYKGGLRFHPSVNLGILKFLAFEQVFKNSLTTLPMGGGKGGTDFDPKGKSDNEVMRF
                     CQSFMTELWRHIGPDTDVPAGDIGVGGREIGFL"
BASE COUNT         1220 a         2536 c         2493 g         1149 t
ORIGIN      
        1 cgccgtcggc ggcctgaacg aaagggcccc tgggcttgcc cgggggccct tggcgttcgc
       61 gatcctgcac gaacgggccg ctaccccgag agcacctcca ggcgcacgcg cgccgtgcct
      121 tcgcgcagga atccgagatc ctccgccgcg cggcgcgaca cgtcgatgac gcgatggcgg
      181 cggaaggggc cgcggtcggt gacggtcacc acgaccttcc gcccgttgtc gagattcgtg
      241 acccgcacgc gggtgccgaa gggaagggtg cgatgcgccg cggtgaggcg gcccgcgtcg
      301 aaccgctcgc cgctcgccgt ccggcggccg tggtgctccc ggccgtagta ggaggcgtat
      361 ccctcttcct tgaacgcgag ccgttcttcg gatcccgagg gcgcgacggc gatcggaatg
      421 gaccgcgagc cggcgcatcc cggaacgagg ccgaccatga gggcgagtgc gaggattcga
      481 cgcatctcgt actgttcggc atccatcgcc cgggcctgaa caccgatgtg cggccggatc
      541 gaacctggac gccgtatcgg cgcccggcaa ctcaatcagc aacgaactgc cgttcaacca
      601 cttatacccc agttgccgcc gtgtcgcggg cgatcccgcg ttccggcatc acttcgggct
      661 ccagaacacc cccattttcc cgccgcttcc gattcccgta gggcccattt tccatgctac
      721 ttgcggccac cttaccgggg gtcgaatccg gaccgaatat gggttcgacg gggcgcttcg
      781 gcgggccgcc gggcgcttgg gtcgaaaggg gacgaagcca tggccgacgc actctataaa
      841 gaagccctcg aatatcaccg gggcgcccgc cccggaaaga tcgaagtcgt atccacgaag
      901 ccctgcgcca cccagaaaga tctgtcgctc gcgtacaccc ccggagtcgc ggtgccctgc
      961 ctcgagatcg agaagaaccc cgatctcgcg tacgaataca ccgcgcgcgg aaacctcgtc
     1021 gccgtcatct cgaacggcac cgccgtgctc ggactgggcg acatcggtcc gctcgcgggc
     1081 aagccggtca tggaggggaa gggcgttctc ttcaaacgct tcgccgacgt ggacgtgttc
     1141 gacatcgaga tcgcgtcgaa ggacccggaa gagatcgtcc gtctctgcgc gctcctcgag
     1201 ccgacgttcg gcgggatcaa cctcgaggac atcaaggcgc ccgagtgctt caccatcgaa
     1261 cagaagctcc agtcgctcgt ttccattccg gtctttcacg acgaccagca cggcacggcg
     1321 atcatcaccg gcgcggccct cctgaacgcg ctggaggtca ccgggaagaa gcttacggac
     1381 atccgcgtcg tgatcctggg agcgggcgcg tccggcatcg cctccgcgcg ccacttcacg
     1441 aagctcggac tgcgccagga tcagatcatg atgtgcgacc gcaagggggt cgttcacacc
     1501 ggccgcctcg ccgagttgaa tccgcacaag gcggagttcg ccgtcgagac gacggcgcgc
     1561 acggtcgccg acgcgctccg cggcgccgac gtgctgctcg ggctctccag cgcgggactc
     1621 gtgacccagg agatggtgcg gtcgatggcg tccaagccga tcctcttcgc ctgcgccaat
     1681 cccgacccgg agatcagcta ccccgatgcc aaggcggccc gccccgacgt gctcatggcg
     1741 accggccgct ccgactatcc gaaccaggtc aacaacgtcc tcgggttccc gttcctcttc
     1801 cggggagcgc tcgacgtccg ggcccggcgc ttcaacgacg agatgaagct ggccgcgacc
     1861 cgcgcgctgg ccgatctggc gaagcaggac gtgcccgacg cggtggcgcg cgcctacggc
     1921 gtcgaaacgc tgcacttcgg tcccgactac ctgatcccga agccgttcga cccgcgcgtg
     1981 ctgctctggg tcgcgccggc cgtggcgaag gccgccatgg agaccggggt cgcccgcctg
     2041 acgatcgatc tcgacgagta ccgcgaccgg ctggaggcgc ggctcggcaa gtcgcgcgag
     2101 atcgcgcgcg ccgtgatgca ccgcgcgcgc cgttcgccgc ggcggatcgt gttccccgaa
     2161 gggacgcacc cgcgcattct gcgcgtggcg acccacctga tggccgaggg cgtcgcgcgc
     2221 cccgtgctcc tgggcaagcc ggaggagatc gagcgcgaag cccggcacct ggatctgtcg
     2281 ctcgccggga tcgagcgggt gcatccgcgc cgccatccca agcgggaagc gtacgtggac
     2341 gcgctctacc ggatgcggca gcggaaggga ctgaccgcga aggaagccga caccctgctg
     2401 ctcaacccga actacttcgg cacgatgatg gtgcacctgg gggatgcgga cgggctggtc
     2461 tcaggggtga cgcagcacta tcccgacacg atccgccccg cgctccagat catcgggctc
     2521 cgcccgggca tcacgcgcgt gtgcggcgtc tactgcatcg tggcgcgccg gaagtgctgg
     2581 ttcttcgcgg ataccacggt gaacatccag cccaccgccg aggagctcgc ggagatcgcg
     2641 atcctggcgg ccggcgtggc gcgcgagttc ggattcgatc cgcgcgtcgc gatgctctcc
     2701 ttctccagct tcggatcggc caagcacccg ctggtggaca ccgtgcggga ggcggcgcgg
     2761 ctcgtggcga agcgggcgcc ggacctggtc gtggacggcg agatgcagct cgaggccgcg
     2821 gtggagccca ccgtcgccca cgagcacttc cccttcagcc gcatccaggg agacgccaac
     2881 gtcctggtct tcccggatct ccagtcgggc aacctcgcct acaagctgat gcagcgcctg
     2941 ggcggcgcgg agaccatcgg gccgatcctc accggcctgg ccaagccgat ccatatcgtg
     3001 tcgccgacgt ccgacgacaa cgagatcatc cacatcgcct cgatcgccgt tctcgaggcg
     3061 cagctccggg acgaggagcg ggaacgcgcc gcgcggcggc ccgcgattcc ggtggaggag
     3121 ctcgtctgat ggacatcgga atcctgaagg agacctactc gggcgagacc cgcgtcgcgc
     3181 tcatcccctt cgccgtcggg gagctggtgc ggcaggggaa ccgcgtcgtg atccagtcgg
     3241 acgcgggcgc cagctcgggg ttcgacgatc aggcctaccg cgacgcgggc gccacgatcg
     3301 tctattcggc cgaggaagtg atcgggcggt cgcgggtgat cctcaaggtg atcccgccct
     3361 cgctcgagga gtgccgccgg ctggaggagg ggaagatcct cttctcgttc ctgcatctct
     3421 cgtccgcgcc gcggagcctg gtggagcacc tggtgcgccg ccgcgtcacc gcgatcgggt
     3481 tcgaagcgat cggagacgag aacggagacc gccccgtact gacggccatg agcgagatcg
     3541 cgggaaagct cgcgatcccg atcgcggcgc agtacctcca gacgcggcag ggggggcgcg
     3601 ggatcatgct cgacggcatt ccgggcgttc ctccggggat cgtcgtgatc ctgggagccg
     3661 gtgtcgtggg gtcgacggcg gccgtgcgcg ccgccgccac cggcgcccag gtgatcgtgc
     3721 tcgaccggga cgtggagcgc ctccggcgcc tccacgcccg cgtgcccgcc ttcgtgacga
     3781 cggcggtcgc gaccccgtac aacatcgaac ggttcgtgcc gatcgccgat gccttgatcg
     3841 gcgcggtgct gatccccatg gagacgacgc cgcacgtcct ggacgagcgg ctggtccgtt
     3901 ccatgcggcc ccgctcggtc ttcctggacg tctcgatcga ccagggcgga tgctccacca
     3961 cctcgcgtcc caccaacatc ctcgatccca cgtacgtacg cgagggcgtc gtccatttct
     4021 gcgtcccgaa cattccggcg ctcgtcccgc gcaccgcgac ggcggcgctc tcgaacatca
     4081 tcctgccgta tctcaccgag atcgcgaggc tgggtcccga ccgggttctc cacgacgacc
     4141 ccgtgctcgc gcggggcgtg tacacgcacg acggctactg cctcgagccg aagatcgcat
     4201 cggttctgga cctgccgtac cggggcgccg agcgggagag gagctacgat gtcgcccgct
     4261 agcctgaacg cgccggccgt cgcgacgccc acgccccctc gcaccacgct cgattatcac
     4321 gacaagctgg ccacgcccga ggaggcgctt cgcctggtcc ggccgggcga cacggtctac
     4381 gtgcacccgg gctgcgcggc gccgcaaacg ctgctggccg ccctggtcgc gcgcaaggac
     4441 gaccttcacg acgtgcgcgt gatccatctc atgtcccagg ggcgggccga ctacgcgctt
     4501 cccggtatgg agccgcattt ccggcacgtc gccctcttca tcgggaagaa cacccgcgac
     4561 gccgtcaacg acggccgggc ggactacctc ccggtcttcc tctccgagat cccgcggctc
     4621 ttcaccacgc gcacggtccc gctggaggtc gcgctgatcc acgtgtcgcc gcccgacgag
     4681 cacggcttct gcagcttcgg cgtcggggtg gagtgcacca aggcggcggc gtcgacggcg
     4741 cgcgtcgtga tcgcccaggt gaatccccgg atgccgcggg tgctggggga caatttcatc
     4801 cacgtgcgga acgtgacccg gttcgtcgag gtggacgagc cgctggtcga gctcccgcgc
     4861 gtgcggatgg ccgacgcgtt cgacgagatc ggccggaacg tggccgccct ggtcgaggac
     4921 ggctccacgc tgcagctggg catcggcggc atcccggacg cggtgctcca tcatctccgg
     4981 gaccgcaagc acctgggggt gcacaccgag atgttctcgg acggcatggt ggagctggtc
     5041 caggcgggca tcgtcacgag cgagcggaag acgctccacc cggggaagat cgtcgcttcg
     5101 ttcgtcctcg gcacgcagcc gctcttcgac ttcatccatg acaaccccat cgtggagttc
     5161 catccgagcg actacgtgaa cgatccgttc gtgatcgcgc agaacgaccg catggtcgcg
     5221 atcaactccg cgatccagat cgatctcacc ggacaggtct gctccgattc catcggggac
     5281 atggcctact ccggattcgg gggacaggtc gacttcgtcc ggggcgcctc gcgcgcccgg
     5341 ggcggcaagg cgatcatcgc ccttccgtcg accgccaggg gcggcgaggt ctcgcgcatc
     5401 gcgccctatc tcgaccccgg cgccggggtc gtgacgtcgc gcgccgacgt ccatttcgtg
     5461 gccaccgagt tcggggtggc ggacctgtac gggaagtccc tccgcgagcg ggcccgcgcc
     5521 ctgatcgaca tcgcccatcc ccggttccgc gaggagctgg agcgcggcgc gatgaaacgg
     5581 aaactcctct gaccttggcg cggcccggcc gcgcacggag gcaatccatg gcatcgacca
     5641 agaccgcgga cctggcgggt cccggcatca gcacctatga agaggtagag cgcatcctgc
     5701 ccaacgggta ccggtcgctc ctcgacccga aagagaccca gctcgcgatc cacgcgctga
     5761 agctccattt ggaggagaac ctctgcaagg cgctcagcct gttccacgtg gaggtgccgc
     5821 tggtcgtcac gcgcgagagc gggctgaacg attacctgga ccgcgacggc tcgcggaccc
     5881 ccgttgagtt ccggtgcggc ctgggtctgg aagagcggat gcacacgcag gtcgtgcagg
     5941 cggctaccaa gtggaaacgg ctcgcgctcc ggcagttcga gtgcgtgccc ggcgagggca
     6001 tctgcacgga catgcgcgcg gtgcggaagg actatttcct ggatcacgac cacagcgcgt
     6061 acgtggacca gtgggactgg gagaaagcga tccgtcccga gcagcgaaat ctgacgtact
     6121 tgaaagagac cgtccgggcg atctggggcg tgatccacgg cgccgaggtc ttcatccgga
     6181 aggagttccc gaagctccag gcttcgccct tcccgccgct gcccgaggag ctgacgttcc
     6241 tccacgccga ggacctgctc gacatgtacc ccgaccttcc gcgcaagcag cgggagacgg
     6301 cgatcctcca gaagcacccg gccgtgttca tcatcgggat cggctgggtc ttgaaggacg
     6361 gctacccgca cgagatgcgc gcggcggact acgacgactg ggttaccccg acggtctccg
     6421 aggacgggag gccgatgcac gggctgaacg gggacatcct cgtctggaac ccggtcacgc
     6481 agcggcggca cgagctgacg tcgatgggga tccgggtgac caaggagacc ctgcggcagc
     6541 agctggagat cacggggcag ctcgatttcc tgaagctccc ctatcaccag gcgatcctga
     6601 acgacgaaat cccgttgtcc atcggaggcg gcatcggcca gtcgcgcacc gtgatgctcc
     6661 tgctgcggaa ggcgcatctc ggggaagtca gcgtgaccgt gtggccggac gagctgaacc
     6721 ggatctgcga gcgaaagaac attcacgtgc tgcaatgagc ggcttctggt tgtaacgttc
     6781 ggacggaagc agaccgtccg tttcaggaaa ggagcgattc atgagcgggt tggatgtggc
     6841 ggttcgtacc ccggggaaat ccatgacggc caaggagtac gtgcagaacg tgatggccgg
     6901 catcaaggcg aagaaccccg cggagccgga gttccatcag gcgtcgcagg aagtgttcga
     6961 gtccctggtg ccgatgctgg agcgccaccc ggagtaccgg gcggcgaagc tgctcgagcg
     7021 gatcgccgag ccggagcggg tcctcatgtt ccgcgtgccg tggcaggacg atcgcggcga
     7081 agtgcaggtc aatcgcggtt tccggatcga gatgaacagc gcgatcggcc cctacaaggg
     7141 cgggctccgc ttccatccgt cggtcaacct cggcatcctc aagttcctcg ccttcgagca
     7201 ggtcttcaag aactcgctca cgacgctgcc gatgggcggc ggcaagggcg gcacggactt
     7261 cgatccaaag gggaagagcg acaacgaagt gatgcgcttc tgccagagct tcatgacgga
     7321 gctgtggcgg cacatcgggc ccgacacgga tgttcccgcc ggcgacatcg gcgtgggcgg
     7381 ccgtgagatc ggtttcct
//
LOCUS       DASSLZ010000072         7731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_787035, whole genome shotgun sequence.
ACCESSION   DASSLZ010000072 DASSLZ010000000
VERSION     DASSLZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787035"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1500
                     /locus_tag="VFM00_02575"
     CDS             22..1500
                     /locus_tag="VFM00_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7497158.1"
                     /translation="MAPSDTIPATLLASGGKLLIGAEWREAASGKRFPTLNPATAEPL
                     LDVAEGDAPDVDLAVRAARAALDGAWGKLSASERGVLLWKLADLIEKNRDELARLETL
                     DAGKPIAETTRIDIPLTVDVFRYCAGASTKLEGSTIPVSGPFFNYTLREPIGVVGLIV
                     PWNFPILMAARKVAPALAAGNAVVLKPAEESPLTALRLGELALEAGLPPGALNVVPGY
                     GPTAGAALVAHPGVAGIAFTGETGTGRLIMQNASGTLKKLSLELGGKSPNIVLEDADM
                     ETAARAAMAAIFYNKGEVCTAGSRLFVQQGAHDALLEKVVDRANKLVQGDPMDAKTRL
                     GPQISEAQVDRIRRYVEIGSKEGARLALGGEPARVGNGKGYFWKPTIFDAVKNDMTIA
                     REEIFGPVLSVLEFDSFDDAIAMANDTAYGLAAAVWTRDVKKAHQAARRLQAGTVWIN
                     TYNMYDAASPYGGMKSSGFGRESGLAGLEFYSQTKSVWLDLS"
     gene            1526..2332
                     /locus_tag="VFM00_02580"
     CDS             1526..2332
                     /locus_tag="VFM00_02580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7497159.1"
                     /translation="MPEPGSIGAPIAWPVGPLPDAAPSGAAASKPTDLSIILPVYNER
                     ESLPPLIAEIHAALRGTPHTYEILLVDDGSTDGSADVIRTAALESSNVTGIVFEKNAG
                     QSAAFAAGFRFARGEILVTMDADGQNDPADIPILVSALENADVAAGIRARRADGWRRR
                     ASSKIANAVRRAVVGDHIIDTGCSLKAYRRRAVEGIPFFAGAHRFLAGICEMRGSRVA
                     QIPVRHRPRLRGVSKYGVGNRLFRGIRDLIGVRWLRSRLLQFRVREVIHG"
     gene            2325..2621
                     /locus_tag="VFM00_02585"
     CDS             2325..2621
                     /locus_tag="VFM00_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014746196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HET7497160.1"
                     /translation="MDKWFSSAPVWYVVGLVGQILFGSRFFVQWVASEWAKRPVFPRG
                     FWYLSLVGGCALFVYALHQKDPVFAIGQGAGLLVYARNLMLDHRPQAAAVPSAP"
     gene            2690..4228
                     /locus_tag="VFM00_02590"
     CDS             2690..4228
                     /locus_tag="VFM00_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET7497161.1"
                     /translation="MEPTDARYAEIGREMVASGDWLTPRLNGVTHLDKPPVAYWSAAA
                     GMRVLGVNAAGARIGAALAAAFLFWAAARIARRAGVPPRESILAPLVLASSALAFALS
                     RLLATDIFLAAAVAGFYVTWLAPGGRGRLWMFAALGLGFLIKGPVVFVHTLLPLFVAA
                     LWRRDRGLLSGLGRRLGWAIFALVAFPWYVIVAEQTPGLLGWLLRKEIWLRYTSTVHH
                     RPGPPWYFVAILAAGALPWTAAALHGLFRSARGAARAPAPAESALVAWAVVPVLFFSA
                     SGSKLPAYVLPELTAVAILAAGALARAAGVARWGTALLFAALGIAIEAVGPRALAGLV
                     GAQHASTLPLPPLAHLAAAAFGAGAIAAAARMPAAAAIAGLVAWYGLLGGARTIEGPL
                     GSPVQVARIMERTRQPGEPVIELGAFSAGLPFYAGATIPMVDVPRSEAFESPGVERHA
                     FLAESALPELIRRSGRVWVYGPRDRAAREADTLGVRYEVVARTRNRELGLLEPMPPDS
                     VAGR"
     gene            4237..5025
                     /locus_tag="VFM00_02595"
     CDS             4237..5025
                     /locus_tag="VFM00_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET7497162.1"
                     /translation="MSEVLVERRGAVALLTLNRPDVLNALSVSMASALTEALEAQARA
                     SEVRVIVITGEGRAFSSGGDIAFMKKVVDQGGRFEDFEPLVGGGPGVVRAIMHSEKPV
                     LAAVNGVAAGGGMSLALACDARWAAEEARFGQSFVKIGLHPDWGAVYTLPRLVGTARA
                     LELMWTGDLIGAAEAERLGLVSRVLPADRLLPELLEFAARLARGPAVALAEIKHSVRE
                     SPGYSLEEALTRELSAQERCWGTADAREGFKAFLEKREPKFEGR"
     gene            complement(5051..5758)
                     /locus_tag="VFM00_02600"
     CDS             complement(5051..5758)
                     /locus_tag="VFM00_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7497163.1"
                     /translation="MTGEGVPIVVLVIEDEAPIRKFLRVALESQGYDYVEAATGEEGV
                     AQAGTRSPDLVLLDLGLPDRDGLEVVRRIRSWSSVPILVLTARGRERDKVQALDDGAD
                     DYVTKPFSMPELLARMRVALRHRARVGASEEPSIFEEGPLRIDFARRRVFANGAEVSL
                     TPIEFRLVSVLARHAGRVLTHDFLLREVWGPSYTAEHHYLRVYMAQLRHKLEAEPGRP
                     RLLLTEPGVGYRMADGS"
     gene            complement(5755..7275)
                     /locus_tag="VFM00_02605"
     CDS             complement(5755..7275)
                     /locus_tag="VFM00_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HET7497164.1"
                     /translation="MNPRRRAGGYVGVLLILGACTGLAALMQRHFDFAGLTMVYLLGV
                     VVSAVAFGRGPAVLASVLSVAIFDFAFVPPRYTFEVADAQYLVVFAVMLTVAVITGTL
                     TARVREQREESRMRERRTEALYRLTHDLAVRSTVHDVLLAAVERVSELFGARAAALVA
                     DPRGILTIAAGDPAAVASAPDRAAAEWAYSNGQAAGAITGIPPGARGISTPLIAGAKV
                     LGVLSLDASAPGPDAQELLRALSSQTALALERCRLAEDATQIRAQIEVERARSALLSS
                     VSHDLRTPLASIAGAASSLKAGDLPAGVRADLAQMISEEAERLDRLIGNLLDMTRVES
                     GTLQVKKEWHSTEDIVGAALTRLERQLGGRPVRLSLPADLPLVPADDVLLEAVVRNLV
                     ENAHKYSAPDAPIDIAAGIEGAWLRLDVADRGIGLREGEERRVFEKFYRGEPARALPG
                     VGLGLAICRGVVEAHRGTIEAANRTGGGAVFTVRLPLEGAPPGVEPEGAGASRRSR"
     gene            7426..>7731
                     /locus_tag="VFM00_02610"
     CDS             7426..>7731
                     /locus_tag="VFM00_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HET7497165.1"
                     /translation="MFDKLRRALIGAPKNLFDPRIHQHLALMAFFAWVGLGSDGLSSS
                     SYGPEEIYRQLGAHHVLALYLAAAVMGTVFLISASYRQIIEQFPSGGGGYLVATKLLG
                     "
BASE COUNT         1046 a         2814 c         2710 g         1161 t
ORIGIN      
        1 gtactcccgc taggagacga catggccccg agcgacacga ttcccgcgac cctcctcgcc
       61 tccggcggga agctcctgat cggcgccgag tggcgcgagg cggcctccgg caagcgcttc
      121 ccgacgctca atcccgcgac cgccgaaccg ctactggacg tggccgaggg cgacgccccc
      181 gacgtggacc tcgcggtgcg cgccgcgcgc gcggcgctcg acggcgcgtg ggggaagctc
      241 tctgcctccg agcgcggcgt tctcctctgg aagctcgcgg acctcatcga gaagaaccga
      301 gacgagctgg cccgcctcga gacgctggat gccggaaagc cgatcgccga gacgacccgg
      361 atcgacattc ccctaacggt cgacgtgttc cgctactgtg ccggcgcttc gaccaagctc
      421 gaggggagca ccatccccgt gagcggaccc ttcttcaact acacgctccg cgagccgatc
      481 ggcgtcgtgg ggctgatcgt gccctggaac ttccccatcc tgatggccgc gcgaaaggtc
      541 gcgccggcgc tcgccgcggg caacgccgtg gtgctgaagc cggccgagga gtcgccgctc
      601 acggcactcc gcctgggcga gctggcgctg gaagcggggc ttcctccggg cgcgctgaac
      661 gtggtgccgg gatacggccc cacggcgggc gcggcactcg tggcgcatcc cggcgtcgcg
      721 ggaatcgcct tcaccggcga gaccgggacg gggcggctca tcatgcagaa cgcctcgggg
      781 acgctgaaga agctgtctct ggagctggga ggcaagtcgc cgaacatcgt gctcgaggac
      841 gcggacatgg agaccgccgc gcgcgcggcg atggcggcga tcttctacaa caaaggcgag
      901 gtctgcacgg cgggctcgcg gctcttcgtg cagcaggggg cgcacgacgc gctgctcgag
      961 aaggtggtcg atcgcgcgaa caagctggtc cagggcgacc cgatggatgc caagacccgt
     1021 ctcggcccac agatctccga ggcgcaggtg gaccgcatcc gccgctacgt ggagatcgga
     1081 tcgaaggagg gcgcgcgcct cgccctgggc ggagagcccg cgcgggtcgg caacggcaag
     1141 ggctacttct ggaagccgac catcttcgac gccgtgaaga acgacatgac catcgcgcgc
     1201 gaggaaatct tcggtccggt gctctcggtg ctcgagttcg acagcttcga cgacgcgatc
     1261 gcgatggcga acgacaccgc ctacggcctg gccgcggcgg tctggacccg cgacgtgaag
     1321 aaggcgcacc aggccgcgcg ccggctgcag gccggcaccg tctggatcaa cacctacaac
     1381 atgtacgacg ccgcctcgcc ctacggcggc atgaagtctt cggggttcgg ccgcgaatcg
     1441 ggactggccg gcctcgagtt ctactcccag acgaagagcg tctggttgga tctctcgtga
     1501 gggagggcaa agagcccccg ctgccgtgcc ggaacccggt tccataggag cgcccatcgc
     1561 ctggccggtc ggcccgcttc ccgacgccgc cccctccggg gccgccgcat ccaagcccac
     1621 ggacctttcg atcattctgc ccgtctacaa cgagcgcgag agcctgccgc cgctgatcgc
     1681 cgagatccac gccgccctcc gcggcacgcc ccacacctat gaaatcctcc tcgtcgacga
     1741 cggcagcacc gacggcagcg ccgatgtgat ccgcacggcc gcgctcgagt cttcgaacgt
     1801 cacggggatc gtcttcgaga agaacgcggg ccagagcgcc gccttcgccg ccgggttccg
     1861 cttcgcgcgc ggggagatcc tggtcacgat ggacgccgac ggccagaacg acccggccga
     1921 catcccgatc ctggtatcgg cgctggagaa cgccgacgtc gcggcgggca tccgcgcgcg
     1981 gcgcgcggac ggctggcggc ggcgcgcgtc gtcgaagatc gccaacgcgg tgcgccgcgc
     2041 cgtcgtcggc gaccacatca tcgacaccgg ctgctcgctc aaggcctacc gccgccgcgc
     2101 ggtggagggg atccccttct tcgccggcgc gcaccgattc ctcgccggca tctgcgagat
     2161 gcggggctcc cgcgtcgcgc agattccggt ccggcaccgt ccgcgccttc gcggcgtctc
     2221 caagtacggc gtcggcaacc gactgtttcg gggcattcgc gacctgatcg gcgtccgctg
     2281 gctccggtcg cgtcttctac agttccgtgt ccgggaggtc atccatggat aagtggttct
     2341 cttcggcgcc cgtctggtac gtggtggggc tcgtgggcca gattctcttc ggctcgcgat
     2401 tcttcgtaca gtgggtcgcc tcggagtggg cgaagcgccc cgtgtttccg cgcggcttct
     2461 ggtatctgag cctcgtgggc ggctgcgcgt tgttcgtcta cgccctgcac cagaaggatc
     2521 cggtcttcgc catcgggcag ggagcgggac tcctcgtcta cgcgcgcaat ctgatgctgg
     2581 atcacaggcc gcaggccgcc gccgttccca gcgccccctg aggctctccc gtctcgcctg
     2641 ggcggggccg gcgctcgcgc tcgtcctgac gtgcggcgcc ctggggctga tggagccgac
     2701 cgacgcccgc tacgccgaga tcggccgcga gatggtcgcg agcggcgact ggctgacccc
     2761 gcgcctgaac ggcgtcaccc acctggacaa gcccccggtg gcctactggt cggcggccgc
     2821 gggcatgcgg gtgctcggcg tcaacgcggc cggtgcccgc atcggcgcgg cgctcgcggc
     2881 cgcgttcctc ttctgggcag cggcccgcat cgcgcgccgg gccggcgtgc cgccgcgcga
     2941 gtccatcctg gctcccttgg tcctcgcctc gagcgcgctc gcgttcgcgc tctcccggct
     3001 cctcgcgacc gacatcttcc tcgctgcggc cgtcgccggc ttctatgtca cctggctcgc
     3061 gcccggagga cgcggccggt tgtggatgtt cgccgcgctg ggactggggt tcctgatcaa
     3121 gggaccggtc gtcttcgtgc acacgctgct cccgctgttc gtcgcggcgc tgtggcgccg
     3181 ggatcgtggc ctcctctccg ggctcggccg ccgcctgggc tgggcgatct tcgcgctggt
     3241 cgcgtttccc tggtacgtca tcgtggccga gcagacgccc gggcttctcg gatggctcct
     3301 ccggaaggag atctggctcc gctacacgag cacggtccat caccgcccgg ggccgccctg
     3361 gtatttcgtg gcgatcctcg cggcgggggc actgccgtgg accgccgccg cgctgcacgg
     3421 gctcttccgg agcgcgcggg gcgcggcgcg cgcgcccgct ccggccgaat cggcgctggt
     3481 cgcgtgggcg gtcgtgccgg tgctcttctt ctccgcctcg ggttcgaagc ttccggcata
     3541 cgtcctcccc gagctgaccg ccgtcgcgat cctggccgcg ggagcgctcg cgcgcgccgc
     3601 gggcgtggcg cgctggggaa cggcgctgct cttcgccgcg ctcgggatcg cgatcgaagc
     3661 ggtgggcccg cgcgcgctgg ccggcctggt gggggcacaa cacgcctcga ccctgccgct
     3721 cccacccctc gcccacctcg cggccgccgc gttcggcgcc ggcgccatcg cggcggccgc
     3781 gcgcatgccc gccgcggctg cgatcgcggg gctcgtcgcg tggtacggtc tgttgggcgg
     3841 agcgcgtacc atcgagggac cgctcggatc gcccgtgcag gtcgcgcgga tcatggagcg
     3901 gacgcgccaa cccggcgagc cggtcatcga gctgggcgcc ttctcggcgg gactcccgtt
     3961 ctacgcgggc gcgacgatcc cgatggtgga cgttccgcgc tccgaggcgt tcgaatctcc
     4021 gggtgtcgag cgtcacgcgt ttctggcgga gagcgccctc cccgagctga tccgccggtc
     4081 gggacgcgtg tgggtctacg ggccgcggga tcgcgccgcg cgggaggccg acaccctggg
     4141 cgtccgctac gaggtggtgg cccgcacgcg gaaccgcgag ctgggcctgc tcgagccgat
     4201 gccgccggac tccgtcgccg gccgatagga ggaatcatgt ccgaagtgct cgtcgaacgg
     4261 cgcggcgccg tcgcgctcct gacgctgaac cgccccgacg tcctgaacgc gctctccgtc
     4321 tccatggcgt ccgcgctcac ggaggcgctg gaggcgcagg cgcgcgcgtc cgaagtgcgc
     4381 gtcatcgtga tcaccggcga agggcgcgcg ttctcctcgg gcggggacat cgcgttcatg
     4441 aagaaggtgg tggatcaggg gggccggttc gaggacttcg agccgctcgt gggcggcggc
     4501 cccggcgtgg tgcgcgccat catgcactcg gagaaaccgg tgctggccgc ggtgaacggc
     4561 gtggccgccg gcggcgggat gagcctggcc ctggcgtgcg acgcgcgctg ggccgcggag
     4621 gaagcgcgct tcggacagtc cttcgtgaag atcgggctcc atcccgactg gggggccgtg
     4681 tacacgcttc cgcggctggt cggcacggcg cgcgcgctcg agctgatgtg gaccggcgac
     4741 ctgatcggcg ccgccgaggc ggagcggctc gggctggtca gccgcgtcct gcccgccgac
     4801 cggctccttc cggagttgct cgagttcgcg gcgcggctcg cgcgcggtcc ggccgtggcg
     4861 ctcgccgaga tcaagcactc cgtgcgcgaa tcgccgggct actccctgga ggaggccctg
     4921 acacgcgagc tgtcggcgca ggagcgctgc tggggaacgg cggacgccag ggaggggttc
     4981 aaggcgttct tggagaagcg cgagccgaaa ttcgagggac gctgagagaa cgttccgccc
     5041 gacgccgcga tcagctgccg tcggccattc gatagccgac gccgggctcc gtcaacagga
     5101 gccgcgggcg tcccggctcc gcctccagct tgtgccgcag ctgcgccatg tagacgcgca
     5161 ggtagtggtg ctccgccgtg tacgagggcc cccacacttc gcgaagaagg aagtcgtggg
     5221 tgagcacgcg tcccgcgtgg cgcgccagca ccgagaccaa ccggaactcg atcggggtca
     5281 gcgacacttc ggctccgttg gcgaagacgc gtcgccgcgc gaaatcgatc cggagcggcc
     5341 cctcctcgaa gatgctcggt tcctcggagg ccccgacgcg cgcgcgatgc cgcagcgcga
     5401 cacgcatccg ggcgagaagc tcgggcatgg agaagggctt ggtgacgtag tcgtccgctc
     5461 cgtcatccaa cgcctgcacc ttgtcgcgct cccgcccgcg cgccgtgagc accaggatcg
     5521 gtaccgagga ccagctgcgg atccggcgca ccacctcgag cccgtcccga tcaggaagtc
     5581 ccaggtcgag gagcacgagg tcgggcgatc gcgtcccggc ctgcgcgacc ccttcctcgc
     5641 ccgtggcggc ttcgacgtag tcgtacccct ggctctcgag cgcgacccgg aggaacttgc
     5701 ggatcggagc ttcgtcttcg atgacgagca cgacgatcgg taccccctcg ccggtcaccg
     5761 gctcctcctc gacgcccccg ccccctcagg ctccactccc ggaggcgccc cctcgagcgg
     5821 aaggcggacc gtgaacacgg cgccacctcc ggtccggttc gccgcctcga tcgtcccgcg
     5881 atgggcctcg accacgcccc tgcagatcgc gagccccagc cccacgcccg gcagcgcgcg
     5941 cgccggctcg ccgcggtaga acttctcgaa gacgcgccgc tcctccccct cgcgcagccc
     6001 gatgccccgg tcggccacgt ccaggcggag ccaagcccct tcgatgcctg ccgcgatgtc
     6061 gatcggggca tccggcgcgg agtacttgtg cgcgttctcg accaggttcc gcaccacggc
     6121 ctcgagcagc acgtcgtccg cgggcaccag ggggaggtcg gccggcagcg agagccggac
     6181 cgggcgcccg cccagctgtc gctccaggcg ggtcagggcc gcgccgacga tgtcctccgt
     6241 ggagtgccac tccttcttca cctgcagcgt gcccgactcg acgcgggtca tgtcgagcag
     6301 gtttccgatc aggcggtcga gccgctccgc ctcctcggag atcatctgcg ccaggtcggc
     6361 tcgcaccccc gcgggaagat cgccggcctt gagactgctc gcggcgcccg cgatcgaggc
     6421 aagcggcgtg cggagatcat gggacacgga gctgagcagg gcgctgcgcg cgcgctcgac
     6481 ttcgatctgg gcccggattt gggtggcgtc ttcggccagg cggcatcgct ccagcgcgag
     6541 cgcggtctgg ctggagagtg cgcgcaggag ttcctgggcg tcgggtcccg gcgccgacgc
     6601 gtccagggac agaacgccca agaccttcgc gccggcgatg agcggcgtgg aaatcccccg
     6661 cgcgccgggt gggatgcccg tgatcgcgcc ggcggcctgg ccgttggagt acgcccattc
     6721 cgccgcggcg cggtccggag cggacgcgac cgcggccgga tcgcccgccg cgatcgtcag
     6781 gatcccccgc ggatcggcga ccagagccgc cgcccgcgcc ccgaacagct ccgacacgcg
     6841 ctccaccgcg gccagcagca cgtcgtgcac ggtgctccgc acggccaggt cgtgggtcag
     6901 ccggtagagc gcctccgtgc gccgctcgcg catgcgcgat tcctcacgct gctcgcgcac
     6961 acgcgcggtc agggtcccgg tgatcaccgc gacggtgagc atgaccgcga agaccaccag
     7021 gtactgcgcg tcggcgacct cgaaggtgta gcgcggcggg acgaaggcga aatcgaagat
     7081 ggcgacgctc agcaccgaag ccagaacagc ggggccgcgt ccgaacgcca ccgcgctcac
     7141 gaccacgccc aggagataca ccatggtgag gccggcgaag tcgaagtggc gctgcatgag
     7201 ggcggcgaga ccggtgcagg ccccgaggat gagaagcacc cccacgtagc cgcccgcccg
     7261 tcttcgaggg ttcatgacga cacgatccta ctcccttccc ggccccgcgt cagacccccg
     7321 atcgcccgtc ttgacgcgtt cttgatgcgc ggcgcacacc tccgaacgcc gccttgatgg
     7381 gcggttgggc tctactggag gcgttgcgcg acaccgcgcc cggccatgtt cgacaaattg
     7441 cgtcgcgccc tgatcggcgc ccccaagaac ctcttcgatc cgcggatcca tcagcatctc
     7501 gcgctgatgg ccttcttcgc gtgggtggga ctcggctccg acggcctctc ctcctcctcc
     7561 tacgggcccg aggagatcta ccgccagctc ggcgcgcacc acgtcctcgc gctctatctc
     7621 gccgccgcgg tgatggggac cgtgttcctg atctcggcgt cctaccggca gatcatcgag
     7681 cagtttcctt ccggcggcgg cggctacctg gtcgcgacca agctcctggg a
//
LOCUS       DASSLZ010000073        11348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_660384, whole genome shotgun sequence.
ACCESSION   DASSLZ010000073 DASSLZ010000000
VERSION     DASSLZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660384"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1431)
                     /locus_tag="VFM00_02615"
     CDS             complement(166..1431)
                     /locus_tag="VFM00_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET7497166.1"
                     /translation="MSGSAASAGGAALLVERIQEALREDGLAGWLLFDFHGTNPIARA
                     VLGMESAPNAAKTTRRWFYLVPARGEPRKLVHRIEPRALDHLPGSAAIYLTWQELDRG
                     LADLVQDAVRQHGSSGGYAPALAMEYSPSARLPYVSRVDGGTLELVRASGAQVVSSAD
                     LAQRFDGVLDQDARRDHRRTGDLLSGILAAAFERARDAARAENASGALTEVSLQRFLI
                     ERLAERDLISGDPPTVAVNAHSGDPHFSTSPATDLPIRAGDFLLIDIWAKRKAPGAVY
                     ADYTQVAWLGPSPPERHRAAFLAVRDARDAAIARVREALASNAPVRGADVDDAARAVI
                     EGRGLGPRFLHRTGHSIGREVHGNGVHLDNFETRDERRLLDGTLVSVEPGVYFEDFGV
                     RTEVNLLLDRGEAVVTAAPIQQELPALLS"
     gene            complement(1428..2576)
                     /gene="hflX"
                     /locus_tag="VFM00_02620"
     CDS             complement(1428..2576)
                     /gene="hflX"
                     /locus_tag="VFM00_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET7497167.1"
                     /translation="MAPKYATRAAARHERAVLAGRSIRGARPGEELTELRLLAETAGA
                     SVAGEVLQRRGPVRAATFLSKGKIDEVREIAGETRSSLLLLDDDLSPAQARNLEKDLE
                     LKVVDRSGLILDIFARRARTREARLQVELAQLEYLLPRLTRMWEHLSRLGGGIGTRGP
                     GETQLEVDRRRIRERIAKLKRELQGVVRERRVQRKGRRDCFKVSLVGYTNAGKSTLFN
                     ALTRASVYVEDQLFATLDPTTRAFAAEGPGARKQRALLTDTVGFIRKLPPHLIASFRA
                     TLEEVVEADLLLHVVDAAEPDPDGHIEAVESVLESLGALSRPRLLVFNKIDAIPEEER
                     YGLRARYPGAVLVSAAKREGIAELREEALRRLKGEAAHDSGNPAAESE"
     gene            complement(2586..3818)
                     /locus_tag="VFM00_02625"
     CDS             complement(2586..3818)
                     /locus_tag="VFM00_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET7497168.1"
                     /translation="MKRDIDLLLRERGLAAAVVLKGEFPNPTFRYVAGPPAAHMTTGM
                     IVCRPGATPFLLHGGMERDAAVETGFERAEYGDFAYSKILAEESSSLRAYARLIERVL
                     DKVGATKGPVLIGGIMDVGRGYHVLNRLRELMPGITLAEDTDPGLFLKARLTKDADEV
                     LAVRAVGRVCERAFERVRAVIAGGRLEGAKLKDAQGWVTIGRLRAEVRRVFFEAGLEE
                     PHGNIIAMGRDAGVPHNSGNDADVLEEGKPIVIDLFPVQSGGGYYFDVTRTICVGKAP
                     AELRDLHALVREAVDRTMETIHEGSPGRTYQEQVCDLFEARGHKTIRQDERLEEGYVH
                     GLGHGIGLEIHERPNLSGNAANKDRIEAGSLFTVEPGLYYPSRGLGIRIEDVAWVKPD
                     GSVENLTNIPYELEVAPA"
     gene            complement(3864..5054)
                     /locus_tag="VFM00_02630"
     CDS             complement(3864..5054)
                     /locus_tag="VFM00_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7497169.1"
                     /translation="MTSKTLAVYRKEIIDALRDGRSLFAIFVFPFILYPAMMGFMSWI
                     QNKNTEDARALSLRIGMVGEAQIPGLADSLRAVRGVEAISLPAAPVRLEGANVDAVLE
                     VPDGIAARVAAGDSAKVQLVYKESENRSSQAAERLEPVLDQVRRALTVDWARSLGANA
                     QAPPAFTVEKRDVSSKDEMGRFFAALLIPYLLVFLVAAGSMHMAVDATTGEKERSTLE
                     TILATAATRGELVLGKTMAVLTASLTGALTGITGLFLTFNVIAPLIPGAERQSMQFVV
                     GPDKAVLLLLTMIPAAIFISATLVAIGCFARGMREGQAYASYFYMATIFLGLGSFGQQ
                     SPPPSRFFIPVLNTALLQREILTDTVNSLHALTAVGTSCALAAIMLVVAVRLFSNESV
                     LFRT"
     gene            complement(5051..5806)
                     /locus_tag="VFM00_02635"
     CDS             complement(5051..5806)
                     /locus_tag="VFM00_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7497170.1"
                     /translation="MIQVSELSKTFHDRKKGDIPAVRGVSFTARPGQVFGLLGRNGAG
                     KTTTLRMLATILVPTSGTATVAGFDIVQKPAEVRRRIGYLSGDTKLYDRLTGKELLQY
                     FGALAGMTKGAIAARMTELTEPFGLADLLDVRIGRMSTGMKQRVSIARVVFHDPEVLI
                     FDEPTAGLDVIGARNVLRLIQDLKGKGRTVIFSTHIMTEAERICDEIGIIEEGRLLTD
                     GSLESVRSRSATRSLEDVFVDVVGATAAAEERR"
     gene            5993..6322
                     /gene="trxA"
                     /locus_tag="VFM00_02640"
     CDS             5993..6322
                     /gene="trxA"
                     /locus_tag="VFM00_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7497171.1"
                     /translation="MADTVTQVTDSTFESEVLKSSIPVLVDFWAEWCGPCRMVAPVVE
                     EVARQYQGKVKVAKVDVDQNQRIAVQFQIRSIPSLYIFKDGKVAGAIVGAVPKQQITS
                     ALDKVLV"
     gene            complement(6373..9324)
                     /locus_tag="VFM00_02645"
     CDS             complement(6373..9324)
                     /locus_tag="VFM00_02645"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET7497172.1"
                     /translation="MTPTPLPTPAAPPEDRPAPGEAPVALDDDQRAAVEHGDGPLLVV
                     AGAGTGKTTVIARRIAHLVTTGRARPSEILALTFNDKAAAEMQERVDLLVPYGYSDVR
                     VATFHAFGEEVLAGHGLEVGIAPGSTVLDKTAQALFLAERIEKLPLRRYAPLSDPTRY
                     LHALADYFSRAKDEPWTAEELQTRAEARLGDPDPAARDQAERELELALAYQAYNRMLW
                     EAGFLDFGDLLALTHRLFEESPAVLRRFQERFRYVLVDEFQDTNPVQFKIVRRLVERH
                     RNLVVVGDDDQSIYRFRGAHLKNILEFRQHYPGTREIVLRNNYRSTREILTVARRLIL
                     MNRERLEVRYGIAKELTAHSRGVAPQCREFATEAEEARWVADAIERGVADGRRSWRDH
                     AILVRTNEHAEPFLRALDERGISYWFSGSRGLFQRPEVKQLVALLQSLYQDSRPEHLY
                     LLASEGYGVPDEDLGRLMHLLKDTPGTLRGLFRVAARGGVADLSEEGIARIGELLRDL
                     DELESYAKGRRTGEVLYRYLDRRGVLRDLMASRSYEDEGRARNLVKFFHIIRSFEKVA
                     LADRVALFLRHLEAIQQYGEDPAVADLDATSNVVQVLTIHKAKGLEFPVVFLVQAATD
                     RFPTKYRSRSPELPSETEEPGPTERAELSRAAQHREEERRLCYVAFTRAREELIATYA
                     RDYGTPSERTRSLFLSEAFDLGKPVSAKTRRRAVEMLEENKEREAPPEAPRQDSGVPL
                     ALSFRRLDDYASCPLRYRFLHVLGVGAILPPDPRVNFGQAVHRAAAFALARRMAGAPP
                     ALEETLEMFRASWRCAGHLSPEHEAARFQQGVDALRRFHEREIEAGPMPAAVERPFRI
                     GLDDVLLTGRIDRVDEGAEGTVLVDYKTAEMDDEERADQRAKEDLQLSVYALAWREMT
                     GRMPDRVELRFVTAGTSGRAAMTDARIEKTRETIAAVAASIRAGEFQARPSEYACRRC
                     ACRPICRESAV"
     gene            9457..10407
                     /locus_tag="VFM00_02650"
     CDS             9457..10407
                     /locus_tag="VFM00_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497173.1"
                     /translation="MATDAPALAASRELPPVGAYLRSLSALGRRSIPPALPALVFLWF
                     YHFGTALYLELAGAGTSPLGYRDNNALMIHALMKVSAYLPLLALVYTPFLPLQDALLQ
                     GERMSFVSAIRHVLERMVPFVLSVILQILIVAGPIVLIAGILVAAIAPFPDLPKEIVA
                     VIVVAAVFPLLIWLMLSGFFLFFAIPGVVLSGQGAVHSVSASARLVGSHFAGLLLRLI
                     VFFVILFLIVLVATIPAMLLGTIAAWATHADQVFRILSILWTSLVSALTFPFWVAAVL
                     VLYRALVPAEAAEGTPEMALDAAPGPLGSQGEQPAPLPFE"
     gene            10412..>11348
                     /locus_tag="VFM00_02655"
     CDS             10412..>11348
                     /locus_tag="VFM00_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET7497174.1"
                     /translation="MTRSHGAEGPAVVYSGRYYADIGVHVFPMRKFELIRDELVRRGL
                     VRDEEILDPEPATLEQVQRVHDREYIDKLLHGRLSVLEEAILELPYSEALVEASFLCA
                     GGSILAAREALARGAAANLGGGFHHAFPDHGEGFCVFHDVAIAIRELQAKRAIRRATV
                     IDVDVHQGNGTAAIFREDPSVFTFSVHEEENYPAIKPPSDQDVGLETGANGTTYLAVL
                     EHWVPRILERHRPDLVAYVGGADPYEHDQLGRLRLTREDLRARDRYVFGECAARGIPV
                     AVFLAGGYARDLNDTVGIQADMVDEAIRTAGSSRPE"
BASE COUNT         1622 a         4057 c         3922 g         1747 t
ORIGIN      
        1 cgaggacggt tcggagccgc tccttctgct ccacgctgag cgccccggaa gcctcgacgt
       61 tccagagcac ctcgacgcgc gtctccacct tgttcacgtt ctggccgccc gggccgccgg
      121 agcgcgacgt gcggaagacg atctcggcga gcggcgggag ggcggtcacg aaaggagcgc
      181 cgggagctcc tgctggatcg gcgcggcggt caccaccgct tcgccccggt cgaggagcag
      241 gttcacctcg gttcgcacgc cgaaatcctc gaagtagacc ccgggctcca ccgagacgag
      301 cgttccgtcg aggagccggc gctcgtcgcg ggtctcgaag ttgtccaggt gaacgccgtt
      361 tccgtggacc tcgcgtccga tggaatgccc cgtccggtgc aggaaccggg gacccaggcc
      421 gcgcccctcg atcaccgcgc gcgccgcgtc gtccacgtcg gcgccgcgaa ccggcgcgtt
      481 cgacgcgagc gcctcgcgca cccgcgcgat ggccgcgtcc cgcgcgtcgc gcacggcgag
      541 gaacgcggcg cggtggcgct ccggcggcga gggcccgagc caggcgacct gcgtgtagtc
      601 ggcgtagacc gcgcccggcg ccttccgctt ggcccagatg tcgatcagca ggaagtcgcc
      661 ggcccggatc ggaaggtcgg tggccggcga cgtggagaag tggggatcgc ccgagtgcgc
      721 gttcaccgcg acggtcggag gatcgccgga gatgagatcg cgctcggcga gccgctcgat
      781 caggaagcgc tggagcgaga cctccgtcag cgccccggag gcgttttccg cgcgcgccgc
      841 gtcgcgcgcg cgctcgaacg ccgcggccag gatcccgctc aggagatccc ccgtgcgccg
      901 gtgatcccgg cgcgcgtcct gatcgagcac gccgtcgaag cgctgcgcga gatccgccga
      961 cgagacgacc tgcgcccccg acgcgcgcac cagctccagc gtccctccgt ccacgcgcga
     1021 gacgtacggc agccgcgccg acggggagta ttccatggcg agcgcgggcg cgtagccccc
     1081 gctcgagccg tgctggcgca ccgcgtcctg gaccagatcg gccaggccgc ggtccagctc
     1141 ctgccaggtg agatagatcg ccgcgctccc cggcaggtgg tccagcgccc gcggctcgat
     1201 ccggtggacc agcttgcgcg gctcgccgcg cgccgggacc aggtagaacc agcgccgcgt
     1261 ggtcttcgcc gcgttggggg ccgactccat gccgaggacc gcgcgcgcga tcgggttcgt
     1321 cccgtgaaaa tcgaagagga gccaccccgc gagaccgtcc tcgcggagcg cctcctggat
     1381 ccgctcgacg aggagcgcgg cgccgccggc ggatgcggcc gacccgctca ttccgattcc
     1441 gccgcggggt tgccgctgtc gtgggcggct tcgcccttca ggcggcgcag ggcctcctcg
     1501 cgaagctccg cgatcccctc gcgcttggcg gccgagacga gcacggcgcc gggatagcgc
     1561 gccctaagcc cgtagcgttc ctcctcgggg atggcgtcga tcttgttgaa gaccagaagc
     1621 cgcggccggg acagggcgcc cagggattcc aatacggact ccaccgcttc gatgtggccg
     1681 tcggggtcgg gctccgccgc gtccaccacg tgcagcagga ggtcggcctc gaccacctct
     1741 tcgagggtcg cgcggaacga ggcgatgaga tgcggcggga gcttccggat gaaccccacg
     1801 gtgtcggtga gcagcgcccg ctgcttcctg gcccccggac cctcggccgc gaacgcgcgc
     1861 gtcgtgggat ccagcgtggc gaagagctga tcctcgacgt agacggaggc gcgcgtgagg
     1921 gcgttgaaga gcgtcgactt cccggcgttc gtgtagccga ccagcgatac cttgaagcag
     1981 tcccgccgcc ccttccgctg cacgcggcgc tcgcgcacca cgccctggag ctcgcgcttc
     2041 agcttggcga tccgctcgcg gatgcggcgg cggtccacct cgagctgcgt ctcgcccggc
     2101 ccgcgggtgc cgatcccgcc gccgagacgc gacaggtgct cccacatccg ggtgagccgc
     2161 gggaggaggt attccagctg ggccagctcc acctggagcc gcgcctcgcg cgtgcgggcg
     2221 cgccgcgcga agatgtcgag gatgaggccg ctccggtcca cgaccttgag ctcgaggtcc
     2281 ttctcgaggt tccgggcctg ggccggggag aggtcgtcgt cgaggaggag gagggacgag
     2341 cgggtctcgc ccgcgatctc ccgcacctcg tcgatcttcc ccttgctcag gaaggtggcg
     2401 gcgcgcacgg ggccgcgccg ctggagcact tcgcccgcga cgctcgcgcc cgccgtctcc
     2461 gcgaggaggc gaagctcggt cagctcttca ccgggccggg ccccccgtat cgaccgcccc
     2521 gcgagcaccg cgcgctcgtg gcgcgccgcg gcgcgggtgg cgtatttcgg cgccatgctc
     2581 acgggctacg cgggcgcgac ctccagctcg tagggaatgt tcgtgaggtt ctcgaccgag
     2641 ccgtcgggct tcacccaggc cacgtcctcg atccggatgc ccaggccgcg ggaggggtag
     2701 tagagaccgg gctccaccgt gaagaggctc cccgcctcga tccggtcctt gttcgcggcg
     2761 ttcccgctca ggttcggccg ctcgtggatc tcgagcccga tgccgtgccc cagtccgtgc
     2821 acgtagccct cttccagccg ctcgtcctgc cggatcgtct tgtgcccgcg cgcctcgaac
     2881 aggtcgcaga cctgttcctg ataggtgcgc ccggggctcc cttcgtggat cgtctccatc
     2941 gtgcggtcca ccgcctcgcg caccagggcg tgcaggtccc ggagctcggc cggcgccttg
     3001 ccgacgcaga tcgtccgcgt cacgtcgaaa tagtagccgc cgccgctctg caccgggaag
     3061 agatcgatga cgatcggctt cccctcctcc agcacgtcgg cgtcgttgcc ggagttgtgg
     3121 ggcacgcccg cgtcccgtcc catggcgatg atgttgccgt gcggctcctc gaggcccgcc
     3181 tcgaagaaga cgcggcgcac ctcggcgcgg agccgcccga tcgtgaccca gccctgcgcg
     3241 tccttgagct tcgccccctc gagacgcccg cccgcgatga ccgcgcggac gcgctcgaag
     3301 gcgcgctcgc acacgcgccc gacggcccgc accgcaagga cctcgtccgc gtccttggtg
     3361 agccgcgcct tgaggaagag cccgggatcg gtgtcctccg ccagggtgat ccccggcatc
     3421 agctcgcgga gccggttcag cacgtgatag ccgcgcccga cgtccatgat cccgccgatc
     3481 agcacggggc ccttcgtcgc gccgaccttg tccagcacgc gctcgatcag gcgcgcgtag
     3541 gcccgcagcg aggaggattc ctcggcgagg atcttggagt aggcgaagtc gccgtactcg
     3601 gcgcgctcga agccggtctc caccgcggcg tcgcgctcca tgcctccgtg caggaggaac
     3661 ggggtcgcgc ccgggcggca gacgatcatc ccggtggtca tgtgcgccgc gggcggaccg
     3721 gcgacgtagc ggaacgtggg gttgggaaac tcgcccttca ggacgaccgc ggccgcgagg
     3781 ccgcgctcgc gcagcaagag atcgatgtcg cgcttcacgg tggttctccc gtgcgcgccg
     3841 tcacgcggcg gtgcggcggc ggtctaggtg cggaacagca cgctttcgtt ggagaagagc
     3901 cgcacggcca cgaccagcat gatcgccgcg agcgcgcagc tggttccgac ggccgtgagg
     3961 gcgtgcagcg agttcacggt gtcggtgagg atctcgcgct ggaggagcgc ggtgttgagg
     4021 accgggatga agaagcggga cggcggcggg ctctgctgcc cgaagcttcc cagtcccagg
     4081 aagatggtcg ccatgtagaa ataggacgcg taggcctggc cctcgcgcat gccccgggcg
     4141 aagcagccga tcgccacgag cgtcgccgag atgaagatcg cggcggggat catcgtgagg
     4201 agcaggagca ccgccttgtc cggcccgacc acgaactgca tcgactgccg ctccgcgccc
     4261 gggatgagcg gcgcgatcac gttgaaggtg aggaagagcc cggtgatccc ggtgagcgcc
     4321 ccggtcaacg acgccgtcag gaccgccatc gtcttgccca gcaccagctc gccccgcgtc
     4381 gccgcggtcg cgaggatggt ctccagcgtg ctccgttcct tctcgccggt cgtcgcgtcc
     4441 acggccatgt gcatggagcc ggccgcgacc aggaagacga ggagatacgg gatcaggagg
     4501 gccgcgaaga agcgccccat ctcgtccttg gaggagacat cccgcttctc caccgtgaag
     4561 gccggcggcg cctgcgcgtt cgcgccgagc gatcgggccc agtccacggt gagcgcccgg
     4621 cgcacctggt ccaggaccgg ctcgagccgc tccgccgcct gcgacgaccg gttttccgac
     4681 tccttgtaga cgagctggac cttggcgctg tcgcccgcgg ccacgcgcgc cgcgatcccg
     4741 tccggaacct cgagcaccgc gtccacgttc gcgccctcca gccgcacggg cgccgcgggc
     4801 agcgagatcg cctccacgcc ccgcacggcg cgcagcgagt cggccagccc cgggatctgc
     4861 gcctcgccca ccatgccgat ccggagcgag agcgcgcgcg cgtcctcggt gttcttgttc
     4921 tggatccagg acatgaagcc catcatcgcg gggtagagga tgaacggaaa cacgaagatc
     4981 gcgaagaggg agcggccgtc gcgcagcgcg tcgatgatct ccttgcggta gaccgcgagg
     5041 gtcttggagg tcaccgccgc tcctccgcgg cggcggtcgc gccgaccacg tccacgaaca
     5101 cgtcctcgag cgaccgggtc gcggaacggc tccgcacgct ctccagcgac ccgtcggtga
     5161 ggagccggcc ctcctcgatg atcccgatct cgtcgcagat ccgctcggcc tcggtcatga
     5221 tgtgcgtcga gaagatcacc gtccgcccct tgcccttgag atcctggatc aggcgcagca
     5281 cgttccgcgc gccgatcacg tcgagcccgg ccgtgggctc gtcgaagatc agcacctcgg
     5341 gatcgtggaa cacgacgcgc gcgatcgaga cgcgctgctt catgccggtg gacatgcgcc
     5401 cgatgcgcac gtcgaggaga tcggccagcc cgaagggctc ggtcagctcg gtcatgcggg
     5461 ccgcgatcgc gcccttcgtc atgccggcga gcgcgccgaa gtactggagc agctccttcc
     5521 cggtcaggcg gtcgtagagc ttggtgtcgc cggagagata gccgatccgg cgccgcacct
     5581 ccgcgggctt ctgcacgatg tcgaagcccg ccaccgtggc ggtgccgctc gtcggaacga
     5641 ggatcgtcgc gagcatgcgc agcgtggtgg tcttgccggc gccgttgcgt ccgaggaggc
     5701 cgaagacctg tccggggcgg gccgtgaagg agacgccgcg tacggcgggg atgtcgccct
     5761 tcttccggtc gtggaacgtc ttcgaaagct cggagacctg gatcaaggat tctcccggtg
     5821 gcatggcggg aaggctggtg gcggccgagt ctaggggagc ggccgccgcg gtgtcaacgc
     5881 gcgccggaat cccggcttga agcttggcgt ctaaacggtt acgatcgggg accgacgcga
     5941 aaggttcccg taaccacaac gtaatcaagc acttcgaagg agcaggatcc gaatggccga
     6001 caccgttacg caggttaccg acagcacgtt cgagagcgaa gtgctgaaat ccagcatccc
     6061 ggtcctggtc gatttctggg ccgaatggtg cggcccctgc cgcatggtgg ctccggtcgt
     6121 cgaggaagtc gcccgtcagt atcagggcaa ggtcaaggtc gccaaggtgg acgtggacca
     6181 gaatcagcgc atcgcggtcc agttccagat ccgcagcatt cccagcctct acatcttcaa
     6241 agacggaaaa gtcgcgggcg cgatcgtggg cgccgtgccg aagcagcaga tcacgtcggc
     6301 gctggacaag gttctcgtct agagagcggt ccgggccgcc gcgccacgga tggcgcgcgg
     6361 cggcgccggc gttcacacgg ccgattccct gcagatcggc cggcaggcgc agcgccggca
     6421 cgcgtactcg ctgggtcgcg cctggaattc tcccgcccgg atcgaggcgg cgaccgcggc
     6481 gatcgtctcc cgcgtcttct cgatccgcgc gtccgtcatc gccgcccgtc ccgacgttcc
     6541 ggccgtgacg aaccgaagct ccacgcggtc gggcatccgc ccggtcatct cccgccaggc
     6601 cagcgcgtac accgatagct ggagatcctc cttcgcccgc tgatcggccc gctcctcgtc
     6661 gtccatctcc gccgtcttgt agtccacgag caccgtgcct tccgcccctt cgtccacgcg
     6721 atcgatccgt cccgtcagga ggacgtcgtc gagcccgatc cggaagggcc gctccaccgc
     6781 ggcgggcatc ggtcccgcct cgatctcccg ctcgtgaaag cggcgcaggg cgtcgacgcc
     6841 ctgctggaag cgcgcggcct cgtgctcggg cgaaaggtgc ccggcgcacc gccacgaggc
     6901 gcggaacatc tcgagcgtct cctccagcgc gggcggcgcg cccgccatcc ggcgcgcgag
     6961 cgcgaaggcc gcggcgcgat gcaccgcctg gccgaagttc acacgcggat caggcggcag
     7021 gatggcgccg accccaagca cgtggaggaa gcggtagcgg agcgggcagg acgcgtagtc
     7081 gtcgagccgg cggaacgaga gcgcaagcgg cacgccggag tcctggcgcg gcgcttccgg
     7141 cggcgcctcg cgctccttgt tctcctccag catctccacc gcgcggcggc gcgtcttggc
     7201 cgagaccggc tttcccaggt cgaacgcttc cgagaggaag aggctgcgcg tccgctccga
     7261 cggcgtcccg tagtcgcgcg cgtaggtcgc gatcagctct tcccgcgcgc gggtgaaggc
     7321 cacgtagcag agccgccgct cttcttcccg atgctgcgcg gcgcggctca gctcggcgcg
     7381 ctccgtggga ccgggttctt ccgtttcgga gggcagctcc ggcgagcggc tccggtactt
     7441 cgtcggaaac cggtcggtgg cggcctgcac caggaacacg accgggaact ccagcccctt
     7501 cgccttgtgg atcgtcagca cctggaccac gttcgacgtc gcgtccaggt cggccaccgc
     7561 gggatcttcg ccgtactgct ggatcgcctc cagatggcgc aggaagagcg cgacgcgatc
     7621 ggccagcgcg accttctcga acgagcggat gatgtggaag aacttgacca ggtttcgcgc
     7681 ccgcccctcg tcctcgtacg agcgcgacgc catgagatcg cgcaagacac cccggcggtc
     7741 gagatagcgg tacagcactt cgcccgtgcg ccgtcccttc gcgtaggact ccagctcgtc
     7801 cagatcgcga aggagctcgc cgatccgggc aatgccctcc tcggatagat ccgcaacccc
     7861 gccgcgcgcc gcgacccgga agagcccgcg gagggttccc ggcgtgtcct tgagcaggtg
     7921 catcaggcgc cccaggtcct cgtcgggcac gccgtatccc tccgacgcga ggaggtagag
     7981 gtgctcgggg cgcgagtcct ggtagagaga ctggaggagt gccacgagct gctttacctc
     8041 ggggcgctgg aagaggcccc ggctccccga gaaccagtag ctgatcccgc gctcgtccag
     8101 cgcgcgcagg aacggctcgg catgctcgtt cgtgcggacg aggatggcgt ggtcgcgcca
     8161 gctccgccgt ccgtcggcca cgccgcgctc gatcgcgtcg gccacccacc gcgcctcctc
     8221 ggcctcggtc gcgaactcgc ggcattgtgg ggcgacgccg cggctgtggg cggtcagctc
     8281 cttcgcgatc ccgtagcgca cctccaaccg ctcccggttc atgaggatca gccgccgcgc
     8341 gaccgtcagg atctcgcgcg tgctccggta gttgtttcgc agcacgatct cgcgggtgcc
     8401 ggggtagtgc tggcgaaatt cgaggatgtt cttcaggtgg gcgccgcgga agcggtagat
     8461 gctctgatcg tcgtccccca ccaccacgag attgcggtgc cgctcgacca gccggcgcac
     8521 gatcttgaac tgcaccgggt tcgtgtcctg gaactcgtcc acgagcacgt agcggaagcg
     8581 ctcctgaaag cgccgcagca ccgcggggct ctcctcgaag agccggtgcg tcagcgcgag
     8641 caggtccccg aaatccagga agccggcctc ccagagcatc cggttgtagg cctgataggc
     8701 gagcgcgagc tccagctcgc gctcggcctg gtcacgagcg gcgggatccg gatcgccgag
     8761 gcgcgcctcc gcgcgcgtct gcagctcctc cgccgtccag ggctcgtcct tcgcgcggct
     8821 gaaataatca gccagcgcgt gcagatagcg cgtggggtcg gagagcggcg cgtagcggcg
     8881 caggggaagc ttctcgatcc gttccgccag gaagagggcc tgcgcggtct tgtcgagcac
     8941 ggtcgagccg ggcgcgatcc cgacctccag gccgtgcccc gcgagcactt cctcgccgaa
     9001 ggcgtggaag gtggcgaccc gcacgtcgga gtagccgtag gggaccagca ggtccacccg
     9061 ctcctgcatc tcggcggccg ccttgtcgtt gaacgtgagg gccaggatct cggaggggcg
     9121 cgcgcgcccg gtggtgacga ggtgcgcgat ccggcgcgcg atgacggtgg tcttgccggt
     9181 tcccgccccg gccaccacga ggagcgggcc gtcgccgtgc tcgacggcgg cgcgctgatc
     9241 gtcgtccagg gcgaccggcg cctcgccggg cgcgggacgg tcttccggcg gggccgccgg
     9301 ggtcggaagt ggagtcgggg tcatggagat cggggtcgcc tcgcagagtg gtgcgcgagc
     9361 ctcgattcta tcgcgggaag cgcccgaacg cgaggcggac gagcggtcga aatccggagc
     9421 ttgggagctc cgcggcatgg agtacaatgc gcccccatgg ccacggacgc tccggcctta
     9481 gcagcatcgc gcgagctccc ccccgtgggg gcctatctcc gctccctctc cgccttgggg
     9541 cgccgctcga ttccgcccgc ccttccggct ctggtctttc tctggttcta ccatttcggc
     9601 acggcgctct atctggagct ggccggcgcg ggaacctctc cgctcggcta tcgcgacaac
     9661 aacgcgctga tgatccacgc gctgatgaag gtctcggcct acctgccgct gctcgccctg
     9721 gtctatacgc cgttccttcc tctgcaagac gcgctgctcc agggcgagcg gatgagcttc
     9781 gtcagcgcga tccggcacgt cctggagcgg atggttccgt tcgtgctctc ggtcatcctc
     9841 cagatcctga tcgtcgcggg gccgatcgtc ctgatcgcgg gcatcctcgt ggcggccatc
     9901 gctcccttcc ccgacctgcc caaggagatc gtcgccgtga tcgtcgtggc ggcggtcttt
     9961 ccgctcctga tctggctcat gctctccggc ttcttcctct tcttcgcgat tcccggggtc
    10021 gtgctgtccg gccagggcgc cgtccattcc gtctcggcca gcgcccggct cgtcggctcg
    10081 catttcgccg ggctgctcct ccggctcatc gtattcttcg tgatcctctt cctgatcgtg
    10141 ctcgtcgcca ccatccccgc gatgctcctc gggaccatcg ccgcgtgggc cacgcacgcg
    10201 gatcaggtct ttcgcatcct gagcatcctg tggaccagcc tggtgagcgc actcaccttc
    10261 ccgttctggg tcgccgcggt cctcgtgctc taccgcgcgc tcgtccccgc cgaagccgcc
    10321 gaagggacgc cggagatggc actcgacgcc gcgcccggcc cgctcggctc ccagggcgag
    10381 caacccgcgc cgctcccgtt cgagtagacc cgtgacccga agccacggcg ccgagggccc
    10441 ggccgtcgtc tactccggcc gctactacgc cgacatcggc gtccacgtct tcccgatgcg
    10501 caagttcgag ctgatccggg acgagctggt gcggcggggc ctggtgcggg acgaggagat
    10561 cctcgatccc gagccggcca ccctggaaca ggtgcagcgg gtccacgacc gcgagtacat
    10621 cgacaagctg ctgcacggcc gcctctcggt gctggaggag gcgatcctcg agcttcccta
    10681 ctcggaggcg ctcgtcgagg cgtcgttcct ctgcgcgggc gggagcattc tcgccgcgcg
    10741 cgaggcgctg gcgcgcggcg ccgccgccaa cctgggcgga ggcttccacc acgcttttcc
    10801 cgatcatggc gagggcttct gcgtcttcca cgacgtggcg atcgccatcc gcgagctgca
    10861 ggcgaagcgg gcgatccggc gcgcgacggt gatcgacgtg gacgtgcacc aggggaacgg
    10921 aaccgcggcg atcttccgcg aagacccgtc cgtattcacc ttttcggtgc acgaagagga
    10981 gaactacccc gcgatcaagc cgcccagcga tcaggacgtc ggtttggaga ccggcgccaa
    11041 cggaacgacc tatctcgccg tgctcgagca ctgggtgccg cgcatcctgg aacggcaccg
    11101 tcccgacctg gtcgcctacg tcggaggggc cgacccctac gaacacgacc agctgggccg
    11161 cctgcgcctc acgcgcgagg acctgcgcgc ccgggaccgc tacgtgttcg gcgaatgcgc
    11221 cgcccgcggg atccccgtcg cggtcttcct ggccggaggc tacgcccgcg atctcaacga
    11281 cacggtcgga atccaggccg atatggtgga cgaagcgatc cggacggccg gttcgtctcg
    11341 gcccgaac
//
LOCUS       DASSLZ010000074         8631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_913393, whole genome shotgun sequence.
ACCESSION   DASSLZ010000074 DASSLZ010000000
VERSION     DASSLZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913393"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2056
                     /locus_tag="VFM00_02660"
     CDS             <1..2056
                     /locus_tag="VFM00_02660"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET7497175.1"
                     /translation="DIPRHDSTLIAIATDPEATGSSVTLGFKSPHEVTATVGDYRRDL
                     VRSLYSAMLNERFDEIAHRADPPFLRAGAGGGALGRTDDIWELQASVKDGGIENGLAA
                     LLEETARVRAHGFLPSELARAKDSMRADWERIYAERDKSESAGFAREYVSYFLTAEPA
                     PGIETEYAIVKSVLDGISLAEINAVPAQVMRADSRVVLVTAPSKSQVPDEAAVRAVID
                     REAGANPAAWVDATSGKQLMAKRPAPGSVKSRRTVPEIGATVVTLSNGVQVWLKPTNF
                     KADEIVFSATSIGGQSLADSADYAVAALAGSVIGDAGVGGFTSSELDKVLAGKIARVG
                     ASYGDYTQGISGSTRPQDLETALQLTYLTFTQPTRDPDGFAAFQKRTVEFLKDRANSP
                     QAAFSDTVVAVNEGGFYLDRVPTVAQVEALRLDDVLAFHKKRFANAADFTFAFAGNFK
                     VDAILPLLARYLGSLPSRGKPTTHFAPKFPRYPTANLTVRVRQGLEPKSSTRITYFTT
                     GFPIEELDMHRARACASILTDHLRQTLRELMGGTYSAGASYSTLAPVPGYSTMTVAFG
                     SDPARVDTLVQAAISEIRRLREEGPSAADVQKDQEIERRELDVALQQNGVWTGSILTS
                     LQFGIDPRRIAHRRERIDLLTTENLRDTFRKYFPPDRRTVISLLPEAAADPGAGAAPP
                     GQ"
     gene            complement(2088..3359)
                     /locus_tag="VFM00_02665"
     CDS             complement(2088..3359)
                     /locus_tag="VFM00_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7497176.1"
                     /translation="MNVHALSRQLTLAFAVTVLAMAARSHAQIIPLLSEREIFAQATI
                     EGVDTRGNGLSTDPDQFDAFIQTVSPHVEEVGPCDPPTPPDLCTITVCDAYAGQNSTI
                     YSSGIQFSGEATGAWDGVTSSQYKFRSICKFRFRLDIPVDYTIQLYADNGDLPPGAVF
                     AALEGPTPDITFHYTQYGVLETTGTLGPGEYVLRGETQTDFRTEKYTSGGAYSAIFFI
                     NPIDTLGGYAGLPLNQTVACGGTATFAVASPAPAGQLSFQWMHGLTPLTNSSHISGAN
                     STNLTISNACTADAGYYSVVATVIGSNPVITIPSRMAQLAIVTTPTGAPANDVTPARA
                     PSFEPAAPNPFQSETALRYTVPPSTRVVAAVYDASGARVRRLTNAVLSGSGSIAWDGR
                     TQAGERAPTGVYFVHLEAGAVRETKKVVLVR"
     gene            3723..4313
                     /locus_tag="VFM00_02670"
     CDS             3723..4313
                     /locus_tag="VFM00_02670"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497177.1"
                     /translation="MNADGTNQRNLSRNALTEEHPWWSHDGSRILFCSDRTRKGEKDP
                     NEDIFEMHADGTEVCQITRTPEGETYASWSPDGSRIVCRRVYGDGNWEVVLLDAKGRL
                     INNLSQHPAPDAWPVWTPDGTKIIFASLRSGASRIYEIKPDGTGLRMLTPKDGNWDDR
                     QPAISPDGKTLFFARYRWFPGARYEASQIYWTVLQS"
     gene            4253..5578
                     /gene="kynU"
                     /locus_tag="VFM00_02675"
     CDS             4253..5578
                     /gene="kynU"
                     /locus_tag="VFM00_02675"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HET7497178.1"
                     /translation="MVSRRPVRGIPDILDGVAELAAVRPSPRSTHTSSTLRAKARELD
                     ARDSLAPLRERFYCDPDILYFDGNSLGLLSRDAEAALLRALDQWRRLAARAWLEASPP
                     WVDYGERLGDRVAALVGAEPGEVVVTGSTTVNLHHLVAALYRPDEVRHVIVAAATEFP
                     SDLHALTGQIALQGRDPERSLRLIHPREGGLIEEADIESAIGQDTSLLVLSAIHFCTS
                     QRLDMARLTRRAQAAGARVIWDLSHAVGVAPVELNRIGADAAFWCHYKYLHAGPGAVG
                     GLYLNGRHHGIRPALPGWWGQTTEAKFEMSGRHAPAPGAAAWQIGTIPILAAAPLWGA
                     LDVVESAGIGRIREKSVALTSFLIECVDRVLSEPMRVGSPRDPERRGGHVALECLGDA
                     PAILTRLESRGVVADYRPPNVLRLTPAPLTTRFEDVVRMVEVIGKESTK"
     gene            5575..6045
                     /locus_tag="VFM00_02680"
     CDS             5575..6045
                     /locus_tag="VFM00_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7497179.1"
                     /translation="MKATICLWFDKDAEEAARFYAATFPDSKVTAVHKAPADYPSGKK
                     GDVLTVDFTVLGLPCFGLNGGPAFQHSEAFSFQVATNDQAETDRYWNAIVGNGGQESQ
                     CGWCKDRWGISWQITPRVLTEALAAGGAEAKRAFEAMMPMKKIDVATIEAARRG"
     gene            complement(6087..8378)
                     /locus_tag="VFM00_02685"
     CDS             complement(6087..8378)
                     /locus_tag="VFM00_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7497180.1"
                     /translation="MALSPKTRLGMYEILGPLGAGGMGEVYRAKDLRLGRSVAVKVLP
                     PEVASSPDRLARFEREARTVALLNHPNIVTLFSIEDEGGIRFLTMELVEGRTLADLIP
                     PGGLPLPQILDLGIPLADALVAAHGSGVIHRDLKPGNVMVTADGRVKVLDFGLAKVAW
                     TDTSVVGDAVAETVDSPSSATGPLLGTIPYMAPEQIRGVAVDARADLFAMGILLHELA
                     TGRRPFTGATSADVMSSILRDRAEPLTHARPDLPVDLERVVDRCLEKNPRDRSTAVDV
                     RNELRRIRRELEHPASEPRPPGGAASIAVLPFVNRSADPEDEYFSDGLADELLNVLAK
                     IRGLRVAARTSAFHFKGKDTTIAEVGRALRVATVLEGSVRKAGTRIRIAVQLVQVSNG
                     YHLWSGTYDRTLDDIFAVQDDIAQAVVKELRTTLLGEAPDSGASGRAKADVALAARGR
                     GANPEAHRLYLHGRHFMDRTTRADTAKAIDYLKQAVALEPDFALAWAELSAAQSREAD
                     LSWCPIEEGYRRAREAAERSLALEPDLPEGHAALGWIQMSDLNWGRARASIHRALELA
                     PGSARVLRVAGVLAKAEGRLEEAIRLERQAFEQNPLSGGAYHNLGNTLLAGDRFVEAE
                     AAFRNSLELAPARAATRASLAFALAAQGRLDEAVAETMREPLEWARLRGLAILYHAMG
                     RRVQSDEALRELIEKHGTESACQIAEVHGARGEADAAFDWLERAFAQRDPGLADVKPS
                     PHLRSLHGDPRWDVFLEKLGLKD"
BASE COUNT         1439 a         2881 c         2916 g         1395 t
ORIGIN      
        1 cgacattccc cgacatgatt cgaccttgat cgcgatcgct accgatcccg aggcgacggg
       61 gtcgagcgtg acgctcgggt tcaagtcccc gcacgaggtc accgcgaccg tgggggacta
      121 ccgtcgtgat ctcgtgcgca gcctctattc cgcgatgctc aacgagcgat tcgacgagat
      181 cgcgcatcgc gccgatccgc cattcctccg cgccggcgcg ggcggaggcg cgctgggccg
      241 caccgacgac atctgggagc tccaggcgag cgtcaaggac ggcggcatcg agaacggcct
      301 ggccgcgctg ctcgaggaga ccgcacgcgt tcgcgcacac ggattcctgc ccagcgagct
      361 cgcgcgcgcc aaggacagca tgcgcgccga ctgggagcgc atctatgcgg agcgcgacaa
      421 gtcggagagc gcgggcttcg cgcgggagta cgtgagctat ttcctcaccg cggaaccggc
      481 gccggggatc gagacggaat acgcgatcgt gaagagcgtg ctcgacggca tctcactcgc
      541 cgagatcaat gcggttcccg cgcaggtgat gcgggccgac agccgcgtcg tgctcgtcac
      601 cgccccctcg aagagccagg tgcccgatga ggcggcggtg cgcgcggtga tcgaccgcga
      661 ggcaggggcc aacccggccg cctgggtcga tgcgacctcc ggcaagcagc tcatggccaa
      721 gcggccggcg ccgggatcgg tgaagtcgcg gcgcaccgtc ccggagatcg gcgcgaccgt
      781 cgtgacgctc tcgaacggcg tccaggtttg gctcaagccc acgaacttca aggccgacga
      841 aatcgtgttc tcggccacat cgatcggcgg ccagtcgctg gcggactcgg ccgattatgc
      901 cgtcgccgct ctggcgggat cggtgatcgg cgacgcgggt gtcggcggct tcaccagctc
      961 ggagctcgac aaagtgcttg ccggcaagat cgcgcgcgtg ggcgcttcgt atggcgacta
     1021 cacgcagggg atctcgggga gcacgcgtcc gcaggatctc gagacggcgc tccaactcac
     1081 ctatctcacg tttacgcagc ccacccgtga cccggacggc ttcgccgcgt tccaaaaacg
     1141 gaccgtcgag ttcctgaagg accgcgcgaa cagcccgcag gcggcgttct ccgacacggt
     1201 cgtggcggtg aacgaaggcg gtttctacct ggatcgcgtt ccgaccgtgg cccaggtcga
     1261 agcgctgcgg ctggacgatg tgctcgcgtt ccacaagaaa cgtttcgcta acgcggccga
     1321 cttcaccttc gcgttcgccg ggaacttcaa ggtggatgcg atcctgccgc tgctcgcgag
     1381 gtacctcggc tcgctgccgt cgcgggggaa gcccacgacg cacttcgctc caaagttccc
     1441 gcgctatccg accgccaatc tcaccgtacg cgtgcgccag ggcctggagc cgaagagcag
     1501 cacgcggatc acctacttca ccaccggctt cccgatcgag gagctcgaca tgcaccgcgc
     1561 ccgcgcctgc gcgtcgatcc tcaccgacca tctgcggcag acgctgcgcg agctgatggg
     1621 gggcacctac tcggccgggg cgtcctactc gaccctggcg cccgtgccgg gctattcgac
     1681 gatgaccgtc gcctttggca gcgatccggc gcgggtcgac acgctggtcc aggctgcgat
     1741 atccgagatc cgaaggctgc gcgaggaagg cccatccgcg gccgacgtcc agaaagacca
     1801 ggagatcgag cggcgcgagc tggacgtggc gctgcagcag aacggcgtgt ggacgggctc
     1861 gatcctcacg tcgctccagt tcggcatcga cccgcggcgt atcgcccacc ggcgcgagcg
     1921 catcgacctc ttgacgaccg agaacctgcg cgacaccttc cgtaagtact tcccgcccga
     1981 ccgccgcacc gtgatcagcc ttctgcccga ggccgcggcg gatccgggtg cgggtgcagc
     2041 accgccaggg caatagcggg cagggaacag gccgtcacca gcccgggcta ccgcaccaac
     2101 accacctttt tcgtctcgcg gaccgcaccc gcctcgagat gaacgaagta cacgcctgtg
     2161 ggcgctcgct caccggcctg cgtccgccca tcccacgcga tcgaaccgga tcccgacagg
     2221 acggcattcg tcagcctccg gacacgtgcc cccgacgcgt cgtagacggc cgccaccacg
     2281 cgagtggaag ggggcacggt gtagcgaaga gcggtctccg actggaacgg attcggcgct
     2341 gccggctcga aggaaggcgc gcgagccggc gtcacatcgt tcgcgggcgc gcccgtaggc
     2401 gtcgtgacga tggcgagctg cgccatgcgg cttggaatgg tgatgacggg attgctcccg
     2461 atgacggtgg cgaccacgct gtagtagccc gcgtcggcgg tgcaggcgtt gctgatggtc
     2521 aggttcgtcg agttcgcgcc cgaaatgtgg ctgctgttcg tcagcggcgt gagtccatgc
     2581 atccattgaa acgacaactg ccccgctggg gccggggagg cgacggcgaa ggtcgcggtg
     2641 ccgccgcacg ccacggtctg attgaggggt agtccagcgt aaccgccgag cgtatcgatg
     2701 gggttgatga agaagatggc ggagtatgcg ccgccgctcg tgtacttctc ggtccgaaag
     2761 tcggtttgcg tctcgccgcg gagcacgtat tcgcccggtc ccagcgtgcc ggtcgtctcg
     2821 aggacgccgt actgggtgta atggaaggtg atgtcggggg tgggaccctc tagcgccgcg
     2881 aagacagccc ctgggggcag gtctccgttg tccgcgtaca actgaatggt gtaatcgacg
     2941 gggatatcga gccggaatcg aaacttgcag atcgatcgga atttgtattg cgacgaagtc
     3001 actccgtccc acgctcccgt tgcttcaccg gagaactgga taccggacga gtagatcgtc
     3061 gaattctgcc cggcgtacgc gtcacagacg gtgatggtgc ataggtccgg cggggtcggc
     3121 ggatcgcagg ggcccacctc ctcgacgtgc ggactcaccg tctggatgaa tgcgtcaaac
     3181 tgatccggat ccgtgctcag tccgttgccg cgggtgtcca cgccttcgat cgtcgcctgg
     3241 gcgaagatct cccgctcgct gagcagcggg atgatctggg cgtgcgagcg ggcggccatg
     3301 gcgaggacgg tgaccgcgaa ggcgagcgtc agctgtctgg atagcgcgtg tacgttcatg
     3361 gatccccccc ttgcgtggcg gtgcatgtcc cccataacgc gaaaaatcgg gtccaagagg
     3421 ctcacgcccg actaatccag gggtctggag atgtcgggtt cctgcaatgc aggcccccct
     3481 cccattcgta acctcggctc gaatttgaac cgccgccacc cacgaaccgc cgcctggagg
     3541 gaaccctatg gcccgcctct cgagccttgc aggcatcgtt gccgccctct gcatctccgc
     3601 gttccttgtg ccgggcacga tggcgcagca gccgtttcgc tcgcggtgcg ggtgaccaac
     3661 gcctatccat cgatctcgcc ggacggcaag aaggtcgcgt acacgtccaa tgcgggccgc
     3721 gattgaacgc ggatggcacg aaccagagaa acctctcccg aaacgcgctc accgaagagc
     3781 acccatggtg gtcccatgac ggatcgcgaa ttctcttctg ctcggatcgc accaggaagg
     3841 gagagaagga tcccaatgag gacatcttcg aaatgcatgc cgacggaacg gaggtttgcc
     3901 agatcacccg gacgcctgaa ggggagacct acgcatcgtg gtcgcctgac ggctcccgga
     3961 tcgtgtgccg ccgcgtctac ggcgacggca actgggaagt cgtgctcctg gatgcgaagg
     4021 gaagattgat caacaacctg tcccagcatc cggccccgga cgcatggccg gtgtggactc
     4081 ccgacgggac gaagatcatt ttcgcttcgc tccgcagtgg ggcgtcgagg atctacgaga
     4141 tcaagccgga cgggaccggg ctcaggatgc tgacaccgaa ggatggaaac tgggacgatc
     4201 gccagcccgc gatctcgccg gacggaaaga cgctcttctt cgcccgatat cgatggtttc
     4261 ccggcgcccg gtacgaggca tcccagatat actggacggt gttgcagagc tagctgctgt
     4321 ccgaccctct cccaggagca cgcacacgtc gtccactctg cgcgcgaagg caagagagct
     4381 ggacgcgcgc gattctctcg ccccgcttcg agagcggttc tactgcgacc cggacatcct
     4441 ctacttcgac ggaaactcgc tcggcctcct ctcccgcgac gccgaggcgg cgcttctccg
     4501 cgcgcttgat cagtggaggc gattggcggc ccgagcatgg ctcgaggcct ctccaccatg
     4561 ggtggactat ggagagcgcc tgggagaccg ggtcgcggcc ctggttggag cggaaccggg
     4621 cgaggttgtg gtgaccggct cgacgaccgt gaaccttcat cacttggtgg cggccctgta
     4681 ccgcccggac gaggtccgtc acgtcatcgt ggccgcggcc accgagtttc cctcggacct
     4741 gcacgcgctg acgggccaga tcgcgctcca agggcgcgac cctgagcgct cgcttcgact
     4801 gattcacccc cgcgaagggg gcttgatcga ggaagccgat atcgagtcgg cgatcgggca
     4861 ggacacctcg ctcctcgtgc tttcggcgat tcacttctgc accagccagc ggttggacat
     4921 ggcacgcctg acgcgacggg cgcaggcggc cggcgcgcgg gtgatctggg atctaagcca
     4981 cgccgtcggt gtggcgcccg tcgagctcaa ccgtatcggt gccgacgcgg cgttctggtg
     5041 ccactacaag tacctccatg cgggcccggg cgccgtgggc gggctctatc tgaatggccg
     5101 ccaccatggg attcgaccgg cgctgccggg ctggtggggc caaaccaccg aggccaagtt
     5161 cgagatgagc ggccgccatg ctccggctcc aggcgccgcg gcatggcaga tcgggacgat
     5221 tccgatcctg gcggccgcgc cgctctgggg cgcgctcgac gtcgtggaat ccgccggcat
     5281 cggccggatt cgcgagaaat ccgtcgccct gacgtccttc ttgatcgaat gcgtcgaccg
     5341 cgttctgtcg gaaccgatga gggtggggag cccgcgtgat ccggagcggc gcgggggcca
     5401 tgtcgcgctg gagtgcctcg gggacgcgcc agctatactt accaggctgg aatcgcgcgg
     5461 cgttgtggcc gactatcggc cgccgaacgt gctccggctc acgccggcgc cgctcaccac
     5521 gcgcttcgag gacgtcgtcc gcatggtcga ggtgattgga aaggagtcca cgaaatgaag
     5581 gcaacgatct gtctctggtt cgacaaggat gcagaggagg cggcccgctt ctacgccgcg
     5641 acctttccgg atagcaaggt gaccgcggtt cacaaggctc ccgccgacta cccgagcggc
     5701 aagaagggcg atgtgctgac ggtcgatttc accgttctcg gcctcccctg ctttggtctc
     5761 aacggagggc cggcattcca gcacagcgag gccttctcct tccaggtcgc gacgaacgat
     5821 caggccgaga cggaccggta ctggaacgcg atcgtcggca acggcggcca ggagagccag
     5881 tgcggctggt gcaaggatcg ctggggcatc tcctggcaga tcaccccgcg cgtgctgacc
     5941 gaggcgctcg cggccggtgg tgccgaggca aagcgtgcct tcgaggcgat gatgccgatg
     6001 aagaagatcg acgtcgccac gatcgaggcg gcccggcgag gctgaggtcc atccgcgttc
     6061 gaacgcgatt acgttcgtgg agaaagtcag tccttcaatc ccagcttctc gaggaagacg
     6121 tcccaccggg gatcgccgtg gagcgagcgg aggtgagggc tgggcttgac gtccgcgagg
     6181 cccgggtcgc gctgcgcgaa ggcgcgctcg agccagtcga acgccgcgtc ggcctcgccg
     6241 cgggctccat ggacctcggc gatctgacag gcgctctcgg tcccgtgttt ctcgatcagc
     6301 tcccgaagcg cctcgtccga ttggacccgg cgtcccatgg cgtggtacag gatggccagc
     6361 ccgcgcaggc gggcccactc cagcggctcg cgcatcgtct ccgccacggc ctcatccaga
     6421 cgtccctggg ccgccagggc gaacgcgagg gaagcccgcg tcgcggccct ggccggcgcg
     6481 agctccagag aattccgaaa cgccgcctcc gcttccacga agcggtcgcc ggcgagcagc
     6541 gtgtttccga ggttgtggta cgcgcccccg ctgagagggt tctgctcgaa ggcctggcgt
     6601 tcgagtcgaa tcgcctcctc caggcggccc tccgccttgg cgagcacgcc ggcgacccgg
     6661 agcacccggg cgctcccggg cgcgagctcc agcgcgcggt ggatggaggc cctcgcacgc
     6721 ccccagttca gatcggacat ctggatccat cccaacgccg cgtgcccctc cggtagatcg
     6781 ggctccagcg cgagcgagcg ttcggccgcc tcccgggcgc gtcgatagcc ttcctcgatc
     6841 gggcaccagc tcaagtcggc ctctcgggac tgcgccgcgc tcaactcggc ccatgccagc
     6901 gcgaaatccg gctcgagggc gacggcctgc ttcaggtagt cgatcgcctt cgccgtgtcg
     6961 gcacgggtgg tccgatccat gaagtgccgt ccatggagat agagacggtg cgcctcggga
     7021 ttcgcgccgc gcccccgggc ggccagggcg acgtccgcct tcgcgcgccc gctcgcgccc
     7081 gagtcgggcg cctcgcccaa cagggtcgtg cggagctcct tcaccacggc ctgagcgatg
     7141 tcgtcctgca cggcgaagat gtcgtcgagc gttcgatcgt acgtcccgga ccagaggtga
     7201 tatccgttcg atacctggac cagctgcacc gcgattcgaa tccgagtccc ggccttgcgc
     7261 acgcttcctt ccagcaccgt ggcgactcga agcgcgcgtc ccacttcggc gatggtggtg
     7321 tccttgccct tgaagtggaa cgccgaagtg cgcgcggcca cgcgcaggcc ccggatcttc
     7381 gcgaggacgt tcaggagctc atccgcgagg ccgtcggaga agtattcgtc ctcgggatcg
     7441 gcacttcgat tcacgaacgg aaggaccgcg atcgaggcgg ccccgccggg cgggcgcggc
     7501 tcggaggccg ggtgctccag ttcgcgccgg atccgccgga gctcgttgcg gacgtccacc
     7561 gccgtcgagc ggtcccgggg attcttctcc aggcatcggt ccacgacgcg ctccagatcg
     7621 accgggagat ccggccgggc gtgggtgagc ggctccgccc gatcccgaag gatggacgac
     7681 atcacgtccg ccgaagtggc cccggtgaac ggccggcgac cggtcgccag ctcgtggagg
     7741 aggatcccca tcgcgaagag atcggcccga gcgtcgacgg cgacgcctcg gatctgctcc
     7801 ggagccatgt aagggatcgt gccgaggagc ggccccgtgg cggagctggg ggaatccacg
     7861 gtctcggcga cggcatcgcc gacgacggac gtgtcggtcc acgcaacctt ggccagcccg
     7921 aaatccagga ccttcacgcg cccgtcggcg gtgaccatca cgttgcccgg tttgaggtcg
     7981 cgatggatga ccccggatcc gtgcgcggca accagcgcat cggcgagggg aatgccgaga
     8041 tcgagaattt gcggaagggg gagcccgccg ggggggatca gatcggcgag ggtccggccc
     8101 tcgacgagct ccatggtgag gaagcggatg ccgccctcgt cctcgatgga gaagagggtg
     8161 acgatgttgg ggtgattgag cagcgcgacc gtgcgcgctt cacgctcgaa tcgggcgaga
     8221 cgatcggggg atgaggcgac ttccggcggc agcaccttca ccgccacgct gcggcccagg
     8281 cgcagatcct tcgcccggta gacttcgccc atgccgccgg ctccaagcgg ccccaggatc
     8341 tcgtacatac caagtcgagt cttgggggaa agcgccatcg gctcctagct tagacgccgg
     8401 gcggtacgga tgtctccata tgattatccg cccgcccgag cgccgagatg cggtccgcat
     8461 cttccgccct atttcagctt agggggctca ccggcttccc gaccgacttc tcccagttga
     8521 cgaacagaaa cccacccggg gaatcgagcg gcggcccgtc cggcgccgcc gtcacctgaa
     8581 gctgaagctc ctcgacgggt cgatcggtgc agtcccagca gtccagcgcc c
//
LOCUS       DASSLZ010000075         6373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1549060, whole genome shotgun sequence.
ACCESSION   DASSLZ010000075 DASSLZ010000000
VERSION     DASSLZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549060"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VFM00_02690"
     CDS             complement(<1..875)
                     /locus_tag="VFM00_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET7497181.1"
                     /translation="MTPRPPDPTRVPPPVRELILRLQGEGDRACLVGGCVRDLIRGTQ
                     VLDWDVGTSAVPERVLALFPQAIPTGLKHGTVTVPLASGPCEVTTFRVESGYSDARRP
                     DKVTFTGDVEQDLARRDFTVNAIAWDPIARAEWDPFGGRGDLARRILRAVGDPATRFQ
                     EDGLRPVRAARFAATLEFEVEPETFAALPTAREQVARVAAERIRDEILKMLQAREPSR
                     GFEVLRRSGLLEIILPELAACVAVPQNRYHAYDVYFHTLYSTDAAPPAKPVVRLAALF
                     HDVGKPATRAEKENG"
     gene            944..1324
                     /locus_tag="VFM00_02695"
     CDS             944..1324
                     /locus_tag="VFM00_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497182.1"
                     /translation="MRSRPRPLHGLPRLVLGGALVAIALGAGQPAAADPFLIPCAREG
                     SWTAPDRQVHVAASLAIAASLRVEGRSRLESVAGTAVIGVAKEIYDATLKPRRLGRGA
                     SRKDLAADLLGALAGVLIVAAVDR"
     gene            complement(1350..2759)
                     /locus_tag="VFM00_02700"
     CDS             complement(1350..2759)
                     /locus_tag="VFM00_02700"
                     /inference="COORDINATES: protein motif:HMM:NF044948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7497183.1"
                     /translation="MSRKLVAYIAIVSGAGAVVLGLSLHALQAVGWMQVAVWILVSLL
                     AESLWIPTLTGKAMESMASAVDLSLLVLLGFMPSVWIVAVAFALANLFFSGRKWYKAV
                     FNAGQNVLSLAAAGLVFEALGGAPLVRAGGLPAFHGIALIVPWAAATVVYFLVNTLFV
                     SGAVALSSGRRLLNTWREEYLYPISLLGSAALFFLSPLVVVSYLALGFFGLIFFFVPL
                     LLIKEAGARYIALEKAKDELVSSERLAAKGEMAAEIAHELNNYLAAISGRAQLLLMGI
                     GKQPVEKTEESARIIAEQARNMGVLVKGLLDFSHKEIRKQPTRLNDLVRRTVEFIVPQ
                     NKYERIAFDLDLAPDLPEASLDPGQIQQVLMNLFSNAADSMNAAGRGDPRIAVRTRVQ
                     GEEIRVVVEDTGPGIAAEVINKLFEPSVTTKPEGHGFGLSTCYRIISNHGGKITAENG
                     PRGAVFTLTIPLERKRLAA"
     gene            complement(2756..3571)
                     /locus_tag="VFM00_02705"
     CDS             complement(2756..3571)
                     /locus_tag="VFM00_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET7497184.1"
                     /translation="MIAGFFQFSPERGRPQRNAARLEEALRSTRTDLLVCPELATTGY
                     LYRDKEALRAVAEPVPSGPTVMRLAAASRETGRAIVFGIAERAGAKIYNSAVAIQPEG
                     PVVCYRKAHLFDTETEVFDRAGASPRHAKVAGADVGIMVCFDWRFPEMARVLALEGAD
                     VIAHPANLVHPYCQDAMITRSLENRVFTITASRTGSETVGDVTLAFTGRSQIVSPRGE
                     RLASADSEEECVRTAEIDPLLARDKKVTARNDLFADRRPELYRALDRPPERAS"
     gene            complement(3660..4391)
                     /locus_tag="VFM00_02710"
     CDS             complement(3660..4391)
                     /locus_tag="VFM00_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497185.1"
                     /translation="MSARGFAGLVAAGAGLLALALGILPRALLGADAAEEEVRASFRR
                     VLASQEARALMAIERSDPFGGEPSRENGSVWILPGRGLRYRSSASGGQEIVIDRQKEL
                     FLLYSPSERTVYRAPYSRAPARLRRLVAEPEQFLRARLDARAEPRSIRGRAHDGYRLR
                     GGSLGDSLPEVSVWMSRDRGTGLPHWVSISSPTDTVWIEFRNWNLASRARPGDLVLSA
                     PPGTKEAPLDPRELLERGGGSGESR"
     gene            complement(4388..5200)
                     /locus_tag="VFM00_02715"
     CDS             complement(4388..5200)
                     /locus_tag="VFM00_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497186.1"
                     /translation="MAMLALAGCGKGPVSLTGGPSGSGEKARARFLEEFAKAEPASRG
                     SGAISLKRGSASEGSMDVRWATEVDSLIVVGYVGPIRALDASYLGDSLYVALRPMDLG
                     LAGPILGEDGLGPHGLRFLARPWDFSPGWIRAAIEHGTVEPWGDGWRVAGVFVGEATN
                     PFGLELSAKGEPRRLRIGGAGGRGTLVTIRYGDPKSYRGGTLPRSVEWERGPAVVRLD
                     IDEYARPGPSRLRHLPPADPQWTMLTLDDPRSRALLRRFLGIGEDGVDGAEP"
     gene            5246..6040
                     /locus_tag="VFM00_02720"
     CDS             5246..6040
                     /locus_tag="VFM00_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HET7497187.1"
                     /translation="METVTIESQKAAGTDPVFWTPPPGALPAGATLAMTTRLGGVSRG
                     PYAELNVGLRVGDREDSVLENIRRVRGALGMDAREPARVRQVHGIDLEVAANALDRAP
                     RADGFLLEAGDPWAAVSVADCAAVALVAEDGARGALVHSGWRGTLDGIAVRAALALER
                     SGSPPETLRVVIGPCIHVCCYPVGPETAGRFPAFLIEPHVSGGFALDLPGAIAASLSG
                     AGVPRARIAIAPECTACLPDRFYSHRRDRGTTGRHWMLLRLAPLAR"
BASE COUNT          932 a         2303 c         2294 g          844 t
ORIGIN      
        1 tcgccgttct ccttctcggc ccgcgtggcc ggcttcccca cgtcgtggaa cagcgccgcc
       61 aggcgcacca ccggcttcgc gggcggcgcg gcgtcggtcg agtagagcgt gtggaagtag
      121 acgtcgtagg cgtggtagcg gttctgcggc accgccacgc aggcggccag ctcgggaagg
      181 atgatctcga gcaggcccga gcggcgcagc acctcgaagc cgcgcgacgg ctcccgcgcc
      241 tggagcatct tgaggatctc gtcgcggatc cgctcggccg ccacgcgcgc gacctgctcc
      301 cgcgccgtcg gaagcgcggc gaaggtctcc ggctcgacct cgaactccag ggtggcggcg
      361 aagcgcgcgg cgcgcaccgg acggagcccg tcctcctgaa agcgcgtcgc cggatcgccc
      421 acggcgcgca ggatccgccg ggccagatcg ccgcggccgc cgaacggatc ccactccgcc
      481 cgcgcgatcg ggtcccacgc gatggcgttc accgtgaaat cgcggcgcgc gaggtcctgc
      541 tccacgtcgc cggtgaacgt gaccttgtcc ggccggcgcg cgtccgaata gccggactcg
      601 acgcggaagg tggtcacctc gcagggaccg ctcgccagcg gcaccgtgac cgtaccgtgc
      661 ttcaaaccgg tcgggatcgc ctgtgggaag agcgcgagca cgcgctcggg aacggcgctg
      721 gtccccacgt cccaatcgag cacctgggtt ccgcggatga gatcccgcac gcagccgccc
      781 acgaggcacg cgcggtcccc ttccccctgg aggcggagga tcagctcgcg cacgggcgga
      841 ggcacccggg tcggatcggg agggcgaggc gtcatggcac gctcgatggt agcagagcgc
      901 gcgagcgttg cggcccgcga ggggagcggc taggctcggc tccatgcgct ctcgcccacg
      961 acccctccac ggcctcccgc gcctcgttct cggcggggct ctcgtcgcga tcgccctggg
     1021 cgcgggccag ccggccgccg ccgacccctt cctcatcccc tgcgcgcgcg agggatcctg
     1081 gaccgcgccc gaccgccagg tccacgtcgc ggcgtcgctc gcgatcgcgg cgtcgcttcg
     1141 cgtcgagggg cgctcgcggc tggaatcggt cgccggaacg gccgtgatcg gcgtggcgaa
     1201 ggagatctac gacgccacct tgaagccgag gcggctggga cgcggggcga gccggaagga
     1261 cctggccgcc gatctcctgg gcgcgctcgc gggggtgctg atcgtggcgg ccgtcgatcg
     1321 ctgacggacg gcccgcggcc gcggcggccc tacgccgcca ggcgcttccg ctcgagcggg
     1381 atcgtcaacg tgaacacggc gccgcgcggc ccgttctccg ccgtgatctt cccgccgtgg
     1441 ttcgagatga tccgatagca ggtggagagg ccgaagccgt gtccctccgg cttggtggtc
     1501 accgagggct cgaaaagctt gttgatgacc tcggcggcga tgccgggccc ggtgtcctcc
     1561 acgacgacgc ggatctcctc tccctggacg cgcgtgcgga ccgcgatgcg cgggtcgccc
     1621 cggccggcgg cgttcatcga gtcggccgcg ttggagaaga ggttcatcag cacctgctgg
     1681 atctgaccgg ggtccagcga ggcctcgggc aggtcgggtg ccaggtccag gtcgaacgcg
     1741 atccgctcgt acttgttctg cgggacgatg aactccaccg tgcggcgcac caggtcgttc
     1801 aggcgggtcg gctgcttccg gatctccttg tgcgagaaat cgaggagccc cttcaccagc
     1861 acgcccatgt tgcgcgcctg ctcggcgatg atccgcgcgc tctcctcggt cttctccacc
     1921 ggctgcttgc cgatgcccat caggaggagc tgcgcccgcc cggagatcgc ggcgaggtag
     1981 ttgttcagct cgtgcgcgat ctccgccgcc atttccccct tcgcggcgag gcgctccgag
     2041 gagaccagct cgtccttggc cttctcgagg gcgatgtagc gagcgcccgc ctccttgatc
     2101 aggaggagcg gcacgaagaa gaagatgagt ccgaagaagc ccagcgccag gtacgacacc
     2161 acgacgaggg gcgagaggaa gaagagcgcc gccgagccga gaagcgagat gggatagaga
     2221 tattcctccc gccaggtatt caggaggcgc cgcccgctgg agagcgccac cgcgcccgac
     2281 acgaagaggg tgttcaccag gaagtagacg accgtcgccg cggcccaggg cacgatcagg
     2341 gcgatcccgt ggaacgccgg gagcccgccc gcgcggacga gcggcgcccc tccgagcgcc
     2401 tcgaacacca gccctgcggc ggcgagcgag agcacgttct gccccgcgtt gaagaccgcc
     2461 ttgtaccact tccgtcccga gaagaagagg ttcgcgagcg cgaaggcgac cgcgacgatc
     2521 cagacggagg gcatgaagcc cagcagcacg aggaggctca ggtccacggc cgaggccatg
     2581 ctctccatcg ccttgccggt gagcgtgggg atccagaggc tctcggcgag caggctgacc
     2641 aggatccaga cggcgacctg catccacccg acggcctgga gcgcgtggag cgagagcccg
     2701 agcacgacgg cgcccgcgcc ggacacgatg gcgatgtagg cgaccagctt ccggctcacg
     2761 acgcgcgctc cggcgggcgg tcgagggccc ggtagagctc gggccggcgg tccgcgaaca
     2821 gatcgttccg cgccgtcacc ttcttgtcgc gcgccagcag cggatcgatc tccgccgtgc
     2881 gcacgcactc ctcctcggag tccgccgacg cgaggcgctc gccccggggc gagacgatct
     2941 gactccggcc cgtgaacgcg agggtgacgt cacccacggt ttcgctcccc gtgcggctcg
     3001 cggtgatcgt gaagacccga ttctcgaggg agcgcgtgat catcgcgtcc tgacagtagg
     3061 ggtggacgag gttcgcgggg tgcgcgatca cgtccgcccc ttccagcgcc agcacgcggg
     3121 ccatctcggg aaagcgccag tcgaagcaga ccatgatccc cacgtcggcg cccgccacct
     3181 tcgcgtgccg cggcgaagcc ccggcgcggt cgaagacctc ggtctcggtg tcgaagagat
     3241 gcgctttccg gtagcagacg accggacctt cgggctggat ggccaccgcg ctgttgtaga
     3301 tcttcgcgcc cgcgcgctcg gcgatgccga acacgatcgc gcgcccggtc tcgcgcgagg
     3361 ccgcggcgag gcgcatcacg gtcggacctg acggaaccgg ctcggccacc gcgcgcagcg
     3421 cttccttgtc gcgatagaga tagcccgtgg tggccagctc gggacagacg aggagatcgg
     3481 tcctcgtgga tcggagcgcc tcctccaacc gagcggcgtt ccgttgcggc cgtccccgct
     3541 cgggggagaa ttggaagaag cccgcgatca tcgaagccct gatcgtgcgg cgttaacccg
     3601 ctgggagacg cgaccttctg cggtcacggc cggaccctac cagcggccgc gagccatcgt
     3661 caacgggatt cgcccgatcc tccgccgcgc tcgagcagct cccgcgggtc gagcggcgcc
     3721 tccttggtgc ccggaggcgc ggagaggacc aggtccccgg ggcgcgcccg ggacgcgaga
     3781 ttccagttcc ggaactcgat ccacacggtg tcggtgggcg aggagatcga gacccagtgc
     3841 ggcaggcccg tgccgcggtc gcgcgacatc cagaccgaga cctcgggaag cgaatcgccg
     3901 agcgagccgc cgcgcaggcg gtagccgtcg tgcgcgcgcc cgcggatgga ccgcggttcc
     3961 gcgcgggcgt cgagacgggc gcgaaggaac tgctccggct cggcgaccag gcggcggagg
     4021 cgcgccggcg cgcgcgaata gggcgcgcga tagacggtgc gctcggaggg gctgtagagc
     4081 aggaagagct ccttctgccg gtcgatcacg atctcctggc cgccgctcgc gctggagcgg
     4141 taccggagcc cgcggccggg aaggatccag accgatccgt tctcgcgcga gggctcgccg
     4201 ccgaacggat cgctccgctc gatcgccatc aacgccctgg cctcctgcga cgcgagcacc
     4261 cgacggaagg aggcgcggac ctcctcctcg gccgcgtccg ctccgaggag cgcgcgcggg
     4321 aggatgccca gcgcgagcgc gagaagaccc gcgcccgccg cgacgaggcc ggcgaagccg
     4381 cgcgcgctca aggctccgct ccgtccacgc cgtcctcgcc gatccccaga aaacgccgga
     4441 gcagcgcgcg gctccgcgga tcgtcgagcg taagcatcgt ccactgggga tcggccggtg
     4501 gcaggtggcg cagccgggag gggccggggc gcgcgtattc gtcgatgtcc agccgcacca
     4561 ccgcgggacc gcgctcccat tccacggagc gcggcagcgt tccgccgcgg tagctcttcg
     4621 gatcgccgta gcggatggtg accagcgtgc cgcggccgcc ggccccgccg atccgaagcc
     4681 gccgcggctc tcccttcgcg ctcagctcga ggccgaaggg attcgtcgcc tcccccacga
     4741 acaccccggc cacccgccag ccgtcgcccc agggctccac cgtcccgtgc tcgatcgcgg
     4801 cgcggatcca gccggggctg aaatcccagg gtcgcgcgag gaaccggagc ccgtgcggcc
     4861 ccagcccgtc ctcgcccagg atcgggccgg ccaggccgag atccatgggg cggagtgcca
     4921 cgtagagcga gtcgcccagg taggaggcgt ccagcgcgcg gatcggtccg acgtagccca
     4981 cgacgatgag gctgtccacc tcggtggccc agcgcacgtc catcgagccc tcgctggcgc
     5041 tgccgcgctt gagcgagatc gctcccgatc cgcgcgaggc cggctccgcc ttggcgaatt
     5101 cctcgaggaa gcgcgcacgc gccttctccc ccgagccgga tggcccgccc gtgagcgaga
     5161 ccgggccctt gccgcagccc gcgagcgcca gcatcgccac gcaacccgct tgcgccagcg
     5221 cgcggaggac ggcagagtga cgggaatgga gacggtaacg atcgagagcc agaaggccgc
     5281 cgggaccgac ccggtgttct ggacgccgcc gccgggggcc ctccccgccg gcgcgacgct
     5341 ggccatgacg acgcgcctcg gcggcgtgag ccggggaccc tacgccgagc tcaacgtcgg
     5401 tctccgcgtc ggcgaccgcg aggattcggt gctcgaaaac atccgccggg tccgcggcgc
     5461 gctcgggatg gacgcgcggg agccggcgcg cgtccggcag gtgcacggca tcgatctcga
     5521 ggtcgccgca aacgcgctcg accgggctcc ccgcgccgac ggattcctgc tcgaagccgg
     5581 ggatccctgg gccgcggtca gcgtggccga ctgcgcggcg gtcgccctgg tcgcggagga
     5641 cggggcgcgc ggggcgctgg tgcactccgg ctggcgcggc acgctcgacg gcatcgccgt
     5701 gcgcgcggcg ctcgcgctgg agcggagcgg ctccccgccc gaaacgcttc gcgtcgtgat
     5761 cggtccgtgc atccatgtct gctgctaccc cgtgggtccc gagacggccg ggcgcttccc
     5821 ggcgttcctc attgaacccc atgtgagcgg cggcttcgcc ctcgatctgc cgggagcgat
     5881 cgccgcgtcc ttgtccgggg cgggtgtgcc tcgcgcccgg atcgcgatcg ccccggagtg
     5941 taccgcgtgt ctgccggacc ggttctactc ccatcgccgg gaccggggaa cgacgggacg
     6001 ccactggatg ctgcttcggc tggctccgct cgcgcgctga gggtccgctc cagccgcttc
     6061 agcgctttcc gccagacgaa gccgagcaga agaccgaggg ccgcgccggc caggacgtcc
     6121 gacggccagt ggtcccccac gtagacccgc gagaaggcca ccgctcccgc caccacgtac
     6181 gccgcgaccc gggcctccgg gaagacgtat cccacgatcg gcgccgcggt gaagaagttc
     6241 acggcgtgcg acgacgggaa ggagcggctg tggcgcacgc cgaagagcgg cctcgcgtcg
     6301 gcgatctcca ccgaggggcg cgtgcgcccg acgatcggtt tcagcacctt ggaggagagc
     6361 tggtcggagc agg
//
LOCUS       DASSLZ010000076         5915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_661104, whole genome shotgun sequence.
ACCESSION   DASSLZ010000076 DASSLZ010000000
VERSION     DASSLZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661104"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..2410)
                     /locus_tag="VFM00_02725"
     CDS             complement(89..2410)
                     /locus_tag="VFM00_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497188.1"
                     /translation="MKVNTIVGFPAFPGDSLNVGNDLPTVFFRTFPPAVVRRTSQPTS
                     LTLTPGEPTPVTTTFEETSGNAVTLTSAHIEIWPNADASGAPALDVTVPVAYVIPREG
                     IATVTVPVTVPGDVARQSPDGRIALRITYLGVDETQVPATLDAFSGPVTVALTPALAG
                     NAVIRSAVLEWPLPGAVFGAGDTLKPRAVVIGDGTGYFRSVFYMDGEAVAFEEGFMES
                     GRPVVVETRGSIPTRRFGEHRLQFVVEAPQTVAAQPITFLSTPPANGLTPRGPAPPPA
                     LPDTSRFSLQSTWLADGRSGFRDEESAVTGWASFRGRYGLSATKSLEARVTSRVRFDD
                     VENGSASPEQFLTRYQSTRAAVEWGDLAPALASGAPLLASPVPRRASQAVLTSPTLGT
                     FEGYMAMESHPRSAGGPLREARSDLYAARLTHVFLPDRLRASLYGGYTHEDATPGGVE
                     TATRARVIYGGSATLLLARWTLLGDVASVRHRTIAGVETGRTRTGARGELSGTVARFQ
                     ARAEAFRYQPSLATALNPYAISDRKGFAVDLARPVLRWKAFANYRRESPEDASSGAPS
                     VTAERMSAGASFSLNQDSWVSPSIIRIRQTGSQTDFTQTRLATEFTASEQMGGRTTAR
                     FDVGTFDDEKGVNVKRRVLAGSVVSMRRHPGRVTSTISAGIERDENRDLHLRDMTIQG
                     TLEVRWEASPSRLLIMPFLSYISRDYELQGRSEDRAGGRLQISFLRVPGLGDAALSFQ
                     GRVERIWRLIPEGTHDTDEAVEISLGKRISILP"
     gene            3082..4101
                     /gene="asd"
                     /locus_tag="VFM00_02730"
     CDS             3082..4101
                     /gene="asd"
                     /locus_tag="VFM00_02730"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET7497189.1"
                     /translation="MLGATGTVGQRFVQLLENHPWFELAEVMASDQSAGKRYEEAVGS
                     RWKLTTPIPVAARALRVKGPRDRMSSRILFSALDASVAGELEEHYARTGHLVSSNARN
                     HRMDPLVPLVIPEINRDHLELLERQHYGDGGIVTNPNCSTIVLALALAPLHQRFGVEA
                     VIVTTFQATSGAGYPGVPSLDILGNVVPFVPGEEPKMESETQKILGSLNGRGIDPATF
                     PVSALCHRVPVIDGHLEAVSLRLHGNPSRKQIAETLATFRPLARLDLPSAPSDPIAVR
                     TEEDRPQPRLDADRERGMGVTVGRLRPCPVLQWKFEALGHNTIRGAAGAAILNAEILV
                     RDGRL"
     gene            4085..4699
                     /locus_tag="VFM00_02735"
     CDS             4085..4699
                     /locus_tag="VFM00_02735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7497190.1"
                     /translation="MTAVSEARTTTAGRDIVFVRHGETDWNRERRVQGSEGPALNDAG
                     RDQAKALARSLWEVPLAAVYTSALPRAKETAAYVAGPHSLNIHVDPRLNEIHHGAWEG
                     LSEEELPDLALYRRWREDPTCCVLPGAEPLEAVHERAVLAMKEIVAKHPGEEGLIAVV
                     SHQVVLALLKCYVLDQPWSRIRKNALGVASYEVLTVGEGFAPRS"
     gene            4714..5796
                     /locus_tag="VFM00_02740"
     CDS             4714..5796
                     /locus_tag="VFM00_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497191.1"
                     /translation="MRHKEFVSSVLIGCAPLLLAAVLGCPARATPPAAAAGDSIACVP
                     MMADTGIVDTDPTPSPDGKWLAYTSGQDSLRQIWIKPIDGADRGRQITHDPPSARAMT
                     PTWAPDSKSLLFISTRTKDYNIYSAPLEGGDARAMSDARASNRFAVYAPDGKHIVFPS
                     NRQKPGQIWGFDLYLMDAAGEKSAERPARRLTNNNGSPGHPTWSPDGKWIGYVAKPID
                     TTKVVQVGPGMTMQQGPMFTAYHLWKMPAGGGAEQQLTGKGAESQPTEEIWPTWSPDG
                     KWIAVQRRVGPTDDVWVYEVATGTFYPVTSFGDAGKPTWSPDGKSIWFVRSKGKDQDV
                     WLAKNVTAATLRAKAKLDPRTATAGR"
BASE COUNT          952 a         2017 c         2100 g          846 t
ORIGIN      
        1 cgaagcgtca tcggaatggt tgtattcgcc tgacacggcg cactgagcgc caatgacaat
       61 gccgtgccca tgagcgccat ggtgcgcgtc acgggaggat cgaaatccgc ttccccagcg
      121 agatctcgac cgcctcgtcg gtgtcgtgcg ttccttcggg aatgagccgc cagatccgct
      181 ccactcgtcc ctgaaaggaa agagcggcgt ctccgagacc cggcacgcgg aggaaggaaa
      241 tctggagccg gccgcccgcg cggtcttcgc tgcgtccctg cagctcgtag tcgcgggaga
      301 tgtaggagag gaagggcatg atcaggaggc ggctgggcga cgcctcccac cgcacttcga
      361 gcgttccctg gatcgtcatg tccctgaggt gcagatcgcg gttttcgtcg cgctcgatgc
      421 cggcggagat cgtgctcgtc acgcggcccg gatggcgccg catcgagacg accgagcccg
      481 cgagcacgcg gcgcttcacg ttgacgccct tctcgtcgtc gaacgtgccc acgtcgaagc
      541 gcgcggtcgt ccggccgccc atctgctccg aagcggtgaa ctcggtggcg agccgcgtct
      601 gggtgaagtc ggtctgcgac ccggtctgcc ggatgcggat gatcgagggg gagacccagc
      661 tgtcctggtt gagcgagaag gaagcgccgg cgctcatccg ctcggccgtg accgatggcg
      721 cgccggagga ggcgtcttcc gggctctcgc gccggtaatt ggcgaaagcc ttccagcgga
      781 ggaccggacg ggccagatcc accgcgaatc ccttgcggtc ggagatggcg tacgggttca
      841 gggcggtggc cagcgagggc tggtagcgga acgcctcggc ccgcgcctgg aatcgcgcga
      901 ccgtgccgga gagctcgccc cgtgccccgg tgcgcgtgcg gcccgtctcc acgcccgcga
      961 tggtccggtg gcgcacgctg gccacgtcgc cgaggagcgt ccagcgcgcg aggaggagtg
     1021 tcgccgagcc gccatagatc acccgcgcgc gggtggcggt ctccacaccg cccggcgtgg
     1081 cgtcttcgtg cgtgtagccg ccgtagagcg aggcgcgcag gcgatcgggc aggaacacgt
     1141 gcgtgagccg cgcggcgtac aggtcggagc gggcttcccg cagcgggccg cccgcggagc
     1201 gggggtgcga ctccatcgcc atgtagccct cgaaggtgcc gagggtcggg ctggtgagca
     1261 ccgcctggga ggcgcggcgc ggaaccgggc tcgcgaggag gggcgccccc gaggcgagcg
     1321 cgggagccag atcgccccat tccaccgccg cgcgcgtcga ctgatagcgc gtcaggaact
     1381 gctccggcga cgcgctcccg ttctccacgt cgtcgaagcg gacgcggctg gtcacgcgcg
     1441 cctccaggct cttcgtggcc gagagtccgt agcggccgcg gaacgacgcc cagccggtga
     1501 ccgcgctctc ctcgtcgcgg aagccggacc ggccgtccgc gagccacgtg ctctgcaggc
     1561 tgaagcgcga cgtgtcggga agcgccggcg gaggcgcggg cccccgcggc gtgagcccgt
     1621 tcgcgggcgg cgtcgagagg aacgtgatcg gctgcgcggc gaccgtctgc ggcgcctcga
     1681 ccacgaactg cagccggtgc tcaccgaacc ggcgcgtcgg gatcgagccg cgggtctcca
     1741 cgaccaccgg ccgccccgac tccatgaagc cctcctcgaa cgcgaccgcc tcgccgtcca
     1801 tgtagaagac cgagcggaag tatcccgtcc cgtccccgat caccacggcc cgcggcttca
     1861 gcgtgtcccc ggcgccgaaa acggcgccgg gaagcggcca ttcgagaacg gccgatcgga
     1921 tgacggcgtt tccggccagc gccggggtga gcgcgacggt gaccggaccc gagaaggcgt
     1981 cgagcgtggc gggaacctgt gtctcgtcga cgccgagata agtgatgcgg agcgcgatcc
     2041 ggccgtcggg cgattgccgc gccacgtcgc cggggacggt gacgggcacg gtgacggtcg
     2101 cgatcccctc gcgcgggatc acgtacgcca cggggacggt cacgtcgagc gccggagcgc
     2161 ccgaggcgtc ggcattcggc cagatctcga tgtgcgccga cgtcagggtg accgcgttgc
     2221 cggaggtctc ctcgaaggtg gtcgtcaccg gggtcggctc gccgggcgtc agggtgagcg
     2281 acgtgggttg cgaggtccgg cgcaccacgg ccggcgggaa ggtgcggaag aagaccgtcg
     2341 gcaagtcgtt cccgacgttc agggagtcgc cgggaaaagc cgggaagccg acgatggtgt
     2401 tcaccttcat gccatgttgg tgaccgagtg tcatggggaa cgttggcttg agaaagaccg
     2461 tatcacccag tgctgaccat cggggcacgt ccaagagaag gagccctgtg gaatcaagat
     2521 cagcgacggc aaaacctccg ctcttctggg ttggctggtc gaagacgaag tagagctcgg
     2581 cgttcgcccc aacgtgattg tccccgtcgc gcggatagct gtcgatcatg atgggggctg
     2641 ttgctgaagt cgttcctgtg ccggcgctcc ggctagccaa ggtccgaacg acgccgcccg
     2701 agatgatctg cggggttccg ggggcgatcc cgcgaaaggg agcgacccgg gccctggcgg
     2761 gcggggtcgc gctggccggg gtggaccaga gtggcaggaa cgacagcgcg accagggtga
     2821 ggacgaactg gaaggcggag aggtcccggc gaggcgggag ccagttggta gaccgcaaac
     2881 gaatcagggg cgtcgcctcc tcgagaagcg ccgggaatgg ggttgcggga ccagagcgcc
     2941 caaccgtatc atggagggtc tatggtggca agaaaacgaa ctgcggcaac gacttcgcgg
     3001 cgcaaggcgc ccgttcccaa gaaggcggtc tcccgcccgg cggcgcggcg cggccctcct
     3061 tcgaaacgca ttccggtcgc cgtgctcgga gccaccggaa cggtggggca gcgcttcgtg
     3121 cagctcctcg agaaccaccc ctggttcgag ctggccgagg tcatggcctc cgaccagagc
     3181 gccgggaagc gttacgaaga ggcggtcggg agccgctgga agctcacgac cccgattcct
     3241 gtcgcggcgc gcgccctccg ggtgaaggga ccccgcgacc gcatgtcgtc gcgcatcctt
     3301 ttctccgcgc tcgacgcgtc ggtcgccggg gagctggagg agcactacgc gcgcaccggg
     3361 cacctggtct cgtccaacgc gcggaaccac cggatggatc cgctcgtccc gctcgtcatc
     3421 cccgagatca accgcgacca tctggagctg ctcgagcgcc agcactacgg cgacggcggc
     3481 atcgtcacga atccgaactg ctcgacgatc gttttggccc tggccctggc cccgctccac
     3541 cagcggttcg gggtggaggc ggtgatcgtg accacgttcc aggccacgag cggcgccggc
     3601 tatcccggcg tgccctcgct cgacatcctt gggaacgtcg ttccgttcgt ccccggcgaa
     3661 gagccgaaga tggagagcga gacccagaag atcctgggca gcctgaacgg ccgcggcatc
     3721 gatccggcca ccttcccggt gagcgcgctg tgccatcgcg tgcccgtgat cgacggccat
     3781 ctcgaagcgg tgagcctgcg gcttcacggc aaccccagcc ggaagcagat tgccgagacg
     3841 ctcgccacgt tccgcccgct ggccaggctg gacctgccga gcgcgccctc cgacccgatc
     3901 gcggtgcgca ccgaggagga ccggccgcag ccgcgcctgg acgccgaccg ggagcggggc
     3961 atgggcgtca ccgtcggccg tctccgtccc tgccccgtcc tccaatggaa attcgaggcg
     4021 ctcgggcaca acaccatccg cggcgccgcc ggcgcggcca tcctgaacgc cgagatcctg
     4081 gtccgtgacg gccgtctctg aggcgcgtac gacgaccgcc ggccgcgaca tcgtgttcgt
     4141 tcgccatggc gagaccgact ggaatcgcga gcgccgcgtc cagggctcgg aagggcccgc
     4201 gctgaacgac gccgggcggg accaggcgaa agcgctggcg cgctccctct gggaagtgcc
     4261 gctggccgcc gtctacacca gcgcgctccc gcgcgccaag gagaccgccg cgtacgtggc
     4321 ggggccgcat tcgctcaaca ttcacgtgga tccccgcctg aacgagatcc accacggtgc
     4381 ctgggaaggt ctctccgaag aggagctgcc cgacctggcg ctctaccggc gctggcgcga
     4441 ggatccgacc tgctgcgtcc ttccgggggc cgagccgctc gaggcggtgc acgagcgcgc
     4501 cgtcctggcg atgaaggaga tcgtcgcgaa gcatcccggc gaagagggac tgatcgccgt
     4561 cgtgagccat caggtggttc tcgcgctgct caagtgctac gtgctggacc agccctggag
     4621 ccggatccgg aagaacgcgc tcggcgtcgc gtcgtacgaa gtgctgaccg tgggcgaggg
     4681 gttcgcgccc cggtcctgac gaggagggaa gccgtgcgtc acaaggaatt cgtttcgagc
     4741 gtcctgatcg ggtgcgcgcc gctgttgctc gccgccgtcc tgggctgtcc cgcacgggcc
     4801 acgcctcccg cggcggccgc gggcgacagc atcgcgtgcg tgccgatgat ggccgacacc
     4861 gggatcgtcg acaccgatcc caccccctcg cccgacggaa aatggctcgc ctacacgagc
     4921 ggccaggatt ccctgcgcca gatctggatc aagccgatcg acggcgccga ccgcggccgc
     4981 cagatcacgc acgatcctcc ctcggcgcgc gccatgacgc ccacctgggc gccggactcg
     5041 aaatcgctcc tcttcatctc cacccgcacc aaggactaca acatctatag cgcgccgctg
     5101 gagggtggcg atgcgcgcgc gatgtcggac gcgcgcgcga gcaaccgctt cgcggtctac
     5161 gcgcccgacg gaaagcatat cgtctttccc tctaaccgcc agaagccggg ccagatctgg
     5221 ggcttcgacc tctacctgat ggacgcggcg ggagagaagt cggccgagcg ccccgcgcgc
     5281 cgcctgacga acaacaacgg cagccccggc cacccgactt ggtcgcccga cggcaagtgg
     5341 atcgggtacg tcgccaagcc gatcgatacg acgaaagtcg tgcaggtggg gccgggcatg
     5401 acgatgcagc agggcccgat gttcaccgcc tatcacctct ggaagatgcc cgcgggcggc
     5461 ggcgccgagc agcagctcac cggcaagggc gccgagtcgc agccgaccga ggagatctgg
     5521 cccacctggt cgcccgacgg gaaatggatc gcggtgcagc gccgcgtcgg acccaccgac
     5581 gacgtctggg tgtacgaggt ggcgaccggc acgttctacc cggtcacctc cttcggcgac
     5641 gcgggaaagc cgacctggtc gccggacggg aagtcgatct ggttcgtccg ctcgaaaggg
     5701 aaggaccagg acgtctggct ggcgaagaac gtcaccgccg cgacgcttcg cgccaaggcg
     5761 aagctcgacc cgcgcacggc gaccgccggc cgctagcgca ccacgatcgt tccggtcatc
     5821 cctttcttca tgtggctgtc cacgccgcag tagaagtcgt attcccccgc cttcgtcggc
     5881 gtgaagcgca cgtgcttcgt gtggctgaag gagat
//
LOCUS       DASSLZ010000077         6789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_661794, whole genome shotgun sequence.
ACCESSION   DASSLZ010000077 DASSLZ010000000
VERSION     DASSLZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661794"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2619
                     /locus_tag="VFM00_02745"
     CDS             <1..2619
                     /locus_tag="VFM00_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HET7497192.1"
                     /translation="ALDMAKFPRGGVAHRNILVVLAQFPAEGASPARVPDAKSTPFYY
                     QRLFFSDDPNDGIISLREYYKNQSHGRLIISGQVTSKWVTMPHSYRYYANGTAGLDFS
                     AYPRSAQRAAEDAMAAAYTDFGGKLGFFDNDGPDGTPSSGDDDGYIDAVMVVHPGRGS
                     EIIPDTDPEDPNWALWSHEAGITQFSNCDFTNLNSTPDCVPGYTLGNVKGFLYVLVGE
                     YNDYPGDFAVGTYCHEFGHTLGLVDLYDPRAAGLGFFSLMALGNYLPYAANQVLGSHP
                     GGLDAWSRQYLGFDPPDVVKTPGQYKLAPVSEGGGSLKIWSNGEPGTEYYLVEYRTLN
                     GPDQYLPGDGLVVYHVNDLQNDNLSGPGNYRVSVVQADSLHPLQLEDPGINGNFGDAA
                     DPFPGTLGKRSLTNDTQPNTRSLAGANTGIRIWNIVAGAGDNADSASFDLAFSTKPEL
                     RVASVSMNDGGGDNYPDPNETVQLTVTLRNVGLTSNAVSLTLASPDANATVTTAGSTA
                     PAVAGGATATTNTPFVITVGNPGSLPYDVPLSLSWNDGSASGTETLLFTIGMGSGLAD
                     GFESGAQGWTHVAVTPSNNTTDEWHVSTSRAHGGTSAMKIGSTNPLGTGTNEAQTYAS
                     QEDAALISPAFDLPANSELVFWSWIDAETNGGTGAWDGGRVEISMNGGEWVPLAVDGG
                     YGYVMEFNSGAALRGADVVSGSPQRWRRMVADLTPYSGTARIRFRFATDEANDPRLGT
                     TLAQLRYYEGWYVDDVAVQSRAGGGPVARRIAFRAGPNPYRLNGPSSGAVHFRFSAPD
                     GLPRPGQDPEIRIYDVRGREVGRTVATANPLAPSEFLGTWNPKTQGGANLGSGIYFAR
                     LDFSGKVQTTRLVLVR"
     gene            2623..4992
                     /locus_tag="VFM00_02750"
     CDS             2623..4992
                     /locus_tag="VFM00_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET7497193.1"
                     /translation="MARPLGSAIAAITTIALLAGALLGIGPRGAPFLAPSARAATARG
                     AKKPLGDSLRVAPGKAPSHEPEWAARRRAWLHARSERRAARWTGSLPEMVRALRREAS
                     LESLYLNSVHPEKHGRASNAATDAAWRDAALDAIEDGRDALGLSILAGPARADDALLA
                     LRARAALRRGAPDSGLAILAWPPHVPARKSAAPRRLDLPALFVAGALADSAGKPAAAR
                     AAYWSILRAGDPSPAARRTARLRLGSVLLAGGEPRLAIAVLHPEEDESSEAALLAASA
                     RAAIGDTAGATSHLATFAATRGIPTADRYAAAMRAATWMRGAGADSMGERVWIGLLRA
                     LDYVGEPSTGLALMARRRVPAPDSAAALERADVEAALLAKARRNADAAAAFARLLARR
                     DRPAADRARWALGLARARRSLGAFGAMDSAFVVAASLDSAGSSGESAAWERAREWENR
                     RTGREAAPAFAWAAPRIKTPALAEAARIHEAVNWMRAGALDTARVALDRLGPSSAMGR
                     FWTARLALARGDTAAARTAFADTWRLGPWTYEGLRAAEELRDAGRWPRDSIPFRIAAP
                     SRRLLAPSIGEDPPLRARIWSALGYTSLGRETLLDCARAERSGIGPCAAALEERGFFR
                     AVAAPGDSADLRMEYPPAYALDVFAASAAESLDAAIVWSIMRQETGYLAGARSRAGAL
                     GLLQLLPKTANQLAARPVPPDSLLDPTLNVRLGARYLEKLDREMGDPRGTFAAYNAGE
                     DAVRRWIAEQGAIDDRWVELIPYRETRDYVKQVYAAWRRYEALYGTAVR"
     gene            5010..5588
                     /locus_tag="VFM00_02755"
     CDS             5010..5588
                     /locus_tag="VFM00_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HET7497194.1"
                     /translation="MLLRSELKRKAIHLASLSIPLGYYLTPDLWQRDWERALLAAVIL
                     SLAIEVFRLNHQPTRNVFRHFFGELLRNHEEVSLLGSTYLLIACLLSIHLFPKPIAVL
                     ALAFLIVGDTMAAIVGKWLGRIRIFDKTLEGSLACLVTCYLLTLLVPEIPFRVAVIGA
                     LTATVFELLPIPLDDNFRIPLSAGFAMDFFLR"
     gene            5913..>6789
                     /locus_tag="VFM00_02760"
     CDS             5913..>6789
                     /locus_tag="VFM00_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7497195.1"
                     /translation="MLQLLTRVLGTKHERDVKRMWPTVTQINAEFERLHDLSDDALRA
                     KTQEFRARLLEGETVDDLLPEAFAVVKETCRRLVGTTWDVVGIPITWDMIPFDVQLLG
                     AIVLHEGKIAEMATGEGKTLVATLPIYLNALTGRGVHLVTVNDYLARRDSEWMGRIFT
                     YHGLTVGCIQNQMDFAQRRAAYACDITYGTNNEFGFDYLRDNMARHKDHRVQRGHHYA
                     IVDEVDSVLIDEARTPLIISGPVEHSLQQYDEMKPDVERVVRQQSQLVTRILAEAEEL
                     LRDPAGDEPRGAARGL"
BASE COUNT          990 a         2475 c         2326 g          998 t
ORIGIN      
        1 gctctcgaca tggccaagtt tccgcgcggc ggggtggcgc accggaacat cctggtggtg
       61 cttgcccagt ttccggccga gggagcctcc cccgcccggg tgcccgacgc caagagcact
      121 cccttctact accagcgcct cttcttcagc gacgatccca acgacgggat catcagcctg
      181 cgcgagtact acaagaatca gtcccacggc aggctcatca tctcgggcca ggtgacgtcg
      241 aagtgggtca ccatgccgca ctcctaccgc tactacgcga acggcaccgc ggggctcgac
      301 ttctcggctt acccgcgcag cgcgcagcgc gcggccgagg acgcgatggc cgcggcctac
      361 accgacttcg gcggaaagct cggcttcttc gacaacgacg ggcctgacgg gaccccgagc
      421 agcggggacg atgacggcta catcgatgcc gtcatggtcg tccatccggg acgcgggtcg
      481 gagatcatcc cggacaccga ccccgaggat cccaactggg cgctctggtc gcacgaggcg
      541 ggaatcacgc agttctccaa ctgcgacttc acgaacctga acagcacgcc cgactgcgtc
      601 cccggctaca cgctcgggaa cgtgaagggc ttcctctacg tgctcgtggg cgagtacaac
      661 gactatcccg gcgacttcgc ggtggggacg tactgtcacg agttcgggca caccctgggt
      721 ctcgtggatc tctacgaccc ccgcgccgcc gggctcggtt tcttctcgct gatggcgctc
      781 ggcaactacc tcccgtacgc ggcgaatcag gtgctgggct cgcaccccgg cgggctggac
      841 gcctggtccc ggcagtatct cggcttcgac ccgcccgacg tggtgaagac gcccgggcag
      901 tacaagctgg cccccgtgag cgaaggaggc ggctcgctca agatctggtc caacggagag
      961 ccggggacgg agtactacct ggtcgaatac cgcaccctga acgggcccga ccagtacctg
     1021 cccggcgacg gcctcgtcgt ctaccacgtg aacgatctcc agaacgacaa tctcagcggt
     1081 cccggcaact accgcgtctc ggtggtgcag gccgacagcc tgcatccgct gcagctcgag
     1141 gaccccggca tcaatgggaa cttcggggac gccgccgatc ccttccccgg aacgctcggc
     1201 aagcggagcc tcacgaacga cacgcagccc aacacgcgca gcctggccgg agcgaacacc
     1261 ggcatccgga tctggaacat cgtggcgggc gcgggcgaca acgccgatag cgcctcgttc
     1321 gacctggcct tctccaccaa gcccgagctg cgcgtcgcca gcgtctcgat gaacgacggc
     1381 ggcggagaca actatcccga tcccaacgaa acggtgcagc tcacggtgac cctccggaac
     1441 gtcgggctca cgtcgaacgc ggtctcgctg acgctcgcct cgcccgacgc caacgcgacg
     1501 gtcacgaccg cgggcagcac ggcgccggcg gtcgcggggg gcgctaccgc cacgacgaac
     1561 acgccgttcg tgatcacggt cggcaacccg gggtcgctcc cgtacgacgt gccgctctcg
     1621 ctctcctgga acgacggctc cgcctccggg accgagaccc tcctgttcac gatcgggatg
     1681 ggctccgggc tcgcggacgg cttcgagtcg ggcgcgcagg ggtggacgca cgtcgccgtg
     1741 accccctcga acaacaccac ggacgaatgg cacgtctcga cgtcgcgcgc gcacggcggg
     1801 acgtcggcga tgaagatcgg cagcaccaac ccgctcggca ccggcacgaa cgaagcgcag
     1861 acctacgcct cccaagaaga cgccgcgctc atctcgcccg cgttcgacct gccggcgaac
     1921 tcggagctgg tcttctggtc ctggatcgac gccgagacca acggcgggac cggggcctgg
     1981 gacggcggcc gcgtggagat ctcgatgaac ggcggcgagt gggttccgct cgccgtggac
     2041 ggcggctacg gctacgtcat ggagttcaac tcgggggcgg cgcttcgcgg cgccgatgtc
     2101 gtctccggct cgccccagcg ttggcgtcgc atggtggcgg acctgacgcc ctactcgggc
     2161 acggcgcgca tccgcttccg gttcgccacg gacgaggcga acgacccgag gctgggcacg
     2221 acgctggcgc agctgcgcta ctacgaaggc tggtacgtgg acgacgtcgc cgtgcagtcg
     2281 cgcgcgggcg gcggtccggt cgcgcggcgc atcgcgttcc gcgcgggccc gaatccctat
     2341 cgcctgaacg ggccgtcgtc gggagcggtg cacttccgct tcagcgcgcc ggacggcttg
     2401 ccgcgcccgg ggcaggatcc ggagatccgg atctacgacg tgcgcgggcg cgaggtgggc
     2461 cgcaccgtgg ccaccgccaa tccgctcgct ccctccgagt tcctcggcac gtggaatccg
     2521 aagacccagg gcggcgccaa cctcggctcc ggaatctatt tcgcgcgtct cgacttctcg
     2581 gggaaggtcc agaccacccg gctcgtcctc gtgcggtaga cggtggcgcg cccgctcggc
     2641 agcgccatcg ccgccatcac aacgatcgcc cttctcgcgg gcgcgctcct cggcatcggg
     2701 ccgcggggcg cgcccttttt ggcgccgtcc gcccgggccg cgacggcccg tggggcgaag
     2761 aagcccctcg gcgactcgct ccgcgtggcg ccgggcaagg cgccgtctca cgagcccgag
     2821 tgggcggcgc gacgccgcgc atggctccac gcccgctccg agcggcgggc cgcgcgctgg
     2881 acggggtcgc ttcccgagat ggtgcgggcg ctccggcggg aggcctcgct ggagtcgctc
     2941 tatctgaact ccgtccatcc ggagaagcat gggcgcgcct cgaacgccgc gacggacgcc
     3001 gcctggcggg acgcggccct cgacgccatc gaagacggcc gcgacgcgct gggcctctcc
     3061 atcctcgcgg gcccggcccg cgcggacgac gccctcctcg cgctccgcgc ccgcgccgct
     3121 ctgaggcgcg gggctcccga ttcgggcctg gccatcctcg cctggccgcc ccacgttccc
     3181 gcgcgcaaga gcgccgcgcc ccgccgtctc gatcttcccg cgctcttcgt cgccggggcc
     3241 ctggccgact cggcgggaaa gcccgccgcg gcgcgcgccg cctactggtc aatcctgcgt
     3301 gccggcgatc cctcgcccgc ggcgcgccgt acggcgcgcc ttcgcctggg gtcggtcctc
     3361 ctcgcgggcg gagagccacg gctggcgatc gcggtgctcc atcccgagga agacgagagc
     3421 tccgaagcgg cgcttctcgc ggcctcggcg cgggccgcga tcggggatac cgccggcgcg
     3481 acgtcgcatc tggcgacctt cgccgccacg cgcgggatcc ccaccgccga ccgctacgcc
     3541 gccgccatgc gcgcggccac gtggatgcgc ggcgccgggg ccgactcgat gggcgagcgc
     3601 gtctggatcg ggctccttcg cgcgctcgat tacgtgggcg agccctcgac cgggctcgcg
     3661 ctgatggcca ggcgccgggt cccggctccc gacagcgccg cggcgctcga acgggcggac
     3721 gtcgaggccg cgcttctcgc caaggcgcgc cggaacgccg acgccgccgc ggccttcgcg
     3781 cggctcctcg cgcgccggga ccgtcccgcc gcggaccgcg cgcggtgggc gctgggtctc
     3841 gcgcgcgcgc gccggagcct cggcgcgttc ggcgccatgg acagcgcttt cgttgtcgcg
     3901 gcctcgctcg actccgcggg gtcgtcgggc gagagcgcgg cctgggagcg ggcgcgcgaa
     3961 tgggagaacc gccgcacggg aagggaagcg gctccggcct tcgcgtgggc cgcgcctcgc
     4021 atcaagacgc cggccctggc cgaggcggcc cggatccacg aggcggtgaa ctggatgcgc
     4081 gcgggggcgc tggacacggc gcgcgtcgcg ctggaccggc tgggtccctc ctcggccatg
     4141 ggacgtttct ggacggcgcg gctcgcgctg gcgcgcgggg acaccgctgc ggcgcgcacc
     4201 gcattcgcgg acacgtggcg cctgggcccc tggacctacg aggggctgcg cgcggcggag
     4261 gagctgcgcg acgcggggcg ctggccgcgc gattcgatcc ccttccggat cgcggccccc
     4321 tcgcggcggc tgctcgcgcc ctcgatcggc gaggatcctc cgctgcgggc gcggatctgg
     4381 agcgccctgg gctacacctc gctcggacgg gagacgctcc tcgactgcgc ccgcgcggag
     4441 cggagcggga tcggcccgtg cgcggcggcg ctcgaggagc gcggcttctt ccgcgcggtg
     4501 gccgcgccgg gcgactcggc cgatctccgg atggagtacc ctccggcgta cgcgctggat
     4561 gtcttcgccg cctccgcggc cgagtcgctc gacgccgcga tcgtctggtc gatcatgcgc
     4621 caggagacgg gctaccttgc gggggcccgc tcgcgcgcgg gggcgctggg gctgctccag
     4681 ctgctgccga agacggccaa ccagctcgcg gcccggccgg tcccgcccga ctcgctcttg
     4741 gaccccacgc tcaacgtccg gctgggggcc cgctacctcg agaagctgga ccgcgaaatg
     4801 ggggacccgc gaggcacctt cgcggcatac aatgccggcg aggatgccgt gcgccgctgg
     4861 atcgcggagc agggcgcgat cgacgatcga tgggtcgagc tgatcccgta ccgggagacg
     4921 cgcgactacg tgaagcaggt ctacgccgcg tggcgccggt acgaggcgct ctacggtacc
     4981 gcggtgcgct agggagggcc ggggaccgag tgctccttcg atcggagctg aaacggaaag
     5041 cgatccatct cgcgtcgctc tcgatcccgc tcggctacta cctcaccccc gatctctggc
     5101 agcgcgactg ggagcgcgcg ctgctcgcgg cggtgatcct gtcgctggcg atcgaggtgt
     5161 tccggctgaa ccatcagccg acccggaacg tgttccggca tttcttcggg gagctgctcc
     5221 gcaatcacga agaggtgtcg ctcctcggct cgacgtatct gctcatcgcc tgcctcctct
     5281 cgatccacct cttcccaaag ccgatcgcgg tgctggcgct ggcctttctc atcgtcggcg
     5341 acacgatggc ggccatcgtg gggaagtggc tgggaaggat ccggatcttc gacaagacgc
     5401 tggaggggag cctggcctgc ctggtgacct gctatctgct cacgctgctg gttccggaga
     5461 tcccgttccg ggtggccgtg atcggggcgt tgacggccac cgtcttcgag ctgctcccga
     5521 tccctctgga cgacaacttc cgcatccccc tgtccgcggg gttcgccatg gacttcttcc
     5581 tccgctgagc cggtggcggc atcccggctc ggccctgcgc atcggaatag ggcggaccgg
     5641 aacaacctta gtgttgggtc cgatccggct tgacacccgg cgtgaggggc acctatgctc
     5701 ctccccggac gaaacgtcgc gaggggtttc cttcgaggcg tcaagtccat atctaactga
     5761 tacttgcacg atgacgagac gagcacgaac cccggtggcg ctccgctcct ggggtttcac
     5821 gttatgagcc cctcgcggcg gccgcgaaga ggccccaagc ggtttcggcg agcgcgttta
     5881 gcgcggccgg cgtagaagaa agggcaatcc acgtgttgca actccttacc cgggtcctcg
     5941 gaaccaagca cgagcgcgac gtcaagcgca tgtggccgac cgtgacccag atcaacgccg
     6001 agttcgagcg tcttcacgac ctgagtgacg acgccctgcg cgccaagacc caggaattcc
     6061 gcgcgcggct cctcgaaggg gagaccgtcg acgatctcct ccccgaggcg ttcgccgtcg
     6121 tcaaggagac ctgtcggcgc ctggtcggga ccacctggga cgtagtcggc atccccatca
     6181 cctgggacat gatccccttc gacgtccagc tcctcggagc catcgtcctc cacgagggga
     6241 agatcgccga gatggccacc ggcgagggca agaccctcgt cgccacgctt ccgatctacc
     6301 tgaacgcgct gaccggcagg ggcgtccacc tggtcaccgt gaacgactac ctcgcccgcc
     6361 gcgacagcga gtggatgggg cggatcttca cgtatcacgg cctcaccgtc ggctgcatcc
     6421 agaaccagat ggacttcgcg cagcggcgcg cggcctacgc ctgcgacatc acctacggca
     6481 ccaacaacga attcggcttc gattacctcc gcgacaacat ggcgcgccac aaggaccatc
     6541 gcgtccagcg cggccaccac tacgcgatcg tggacgaggt cgattcggtg ctgatcgacg
     6601 aggcgcgcac ccccctcatc atctcgggcc ccgtcgagca ctcgctgcag cagtacgacg
     6661 agatgaagcc ggacgtggag cgcgtcgtcc gccagcagag ccagctggtc acgcggatcc
     6721 tcgccgaggc ggaggagctt ttgcgtgatc ctgccggaga tgaaccccgc ggagcggcgc
     6781 gaggcctac
//
LOCUS       DASSLZ010000078         4795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_282938, whole genome shotgun sequence.
ACCESSION   DASSLZ010000078 DASSLZ010000000
VERSION     DASSLZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282938"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VFM00_02765"
     CDS             complement(<1..601)
                     /locus_tag="VFM00_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7497196.1"
                     /translation="MAAPGREVDVVIVGGGPAGLSAALVLGRARRRVVLFDHGRYRNA
                     ASRRMHGYLGRDGIPPAALLEVGRREVLAYGVSIRREEVTRIVPVATRRGHTAFRVSL
                     QRGRPVSAKKILLATGVVDRIPPIPGIESFYGTSVHHCPYCDGWEWRDRRLACYGHGA
                     SGEALAHALLTWSRDVVLLTDGASRLSPGELAELRSRGVE"
     gene            complement(618..854)
                     /locus_tag="VFM00_02770"
     CDS             complement(618..854)
                     /locus_tag="VFM00_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7497197.1"
                     /translation="MFNIGPQELFWLFLIVLLIFGAKRIPEIGRSVGRGIQEFKKGMR
                     EVENELQVSDKPAPAPPAPPAATPRETAVEDRKS"
     gene            complement(903..1322)
                     /locus_tag="VFM00_02775"
     CDS             complement(903..1322)
                     /locus_tag="VFM00_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HET7497198.1"
                     /translation="MGSKGKAGSPALDFTIVGLFVLIAFCAVSVALRVGPQVTSRPKQ
                     PIRIELLNGSGRAGLAGELASYLRDGGFDVLEVSNADRSDYRTTLVVNRTEAPEPAKI
                     VAEYLGTRHVIQQQSSQEMIDVTVIVGRDARRFTQAP"
     gene            complement(1338..1871)
                     /locus_tag="VFM00_02780"
     CDS             complement(1338..1871)
                     /locus_tag="VFM00_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET7497199.1"
                     /translation="MERLWAGWRMEYVAPAKRPGRKAKASCLFCDVAARPRGGRNLVL
                     ASTPLTLVMLNLYPYNVGHVMVAPRRHLGSPAGLNVEEAADVNLWLGRVVDALKREYR
                     PHGFNLGANLGRVSGAGVLGHLHWHVVPRWNGDTNFMPVLAETKVLPEAIDRTYRRLR
                     KRLDAPAERTRPRRRAR"
     gene            complement(1871..2632)
                     /locus_tag="VFM00_02785"
     CDS             complement(1871..2632)
                     /locus_tag="VFM00_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET7497200.1"
                     /translation="MSAPDALPPFTALLLAAGMGKRMNSDLPKVLHPALGTPLIGHVL
                     ARLAPLHPDRVVVVVGHREELVREALRGRAIRFVTQAPQLGTGHAVQVAWDEAAPGGP
                     HVLILAGDMPLVRTESLRRLLARQASEGNAVTVLSAELEDPAGYGRVVRDAAGAFVKI
                     VEEKDATPAERAVREVNSGIYVFEKEPLREALGQLRADNAQKEYYLTDTLAILSRAGR
                     RVGIERAADPRECFGVNTPDQLRMVEETLQAWGEG"
     gene            complement(2644..3465)
                     /gene="panC"
                     /locus_tag="VFM00_02790"
     CDS             complement(2644..3465)
                     /gene="panC"
                     /locus_tag="VFM00_02790"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET7497201.1"
                     /translation="MRALSRRARSRRETIAFVPTMGALHEGHLTLLRLARRAHRRVAA
                     SIFVNPLQFGPREDFARYPRPLARDTRLCREAGVDWLFLPTVRALYPPGAETRVVPGP
                     IASRWEGVVRPGHFTGVLTVVMKLLQIVEPDTLILGQKDAQQAALVGAMMRDLDSPAT
                     LRVAPTVRERDGLALSSRNAYLSPGERARAAGLSRALRAGRDQARAGVRNAARITAAA
                     RAVLRREATPDGVDYLALVDPRTFEPLRRLDRRALLIAAIRIGRTRLIDNLAVTP"
     gene            complement(3480..4355)
                     /gene="panB"
                     /locus_tag="VFM00_02795"
     CDS             complement(3480..4355)
                     /gene="panB"
                     /locus_tag="VFM00_02795"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET7497202.1"
                     /translation="MSAGTSRDDRAFERPKVTVRSIREMKARGERIVSVTAYDYPTAR
                     LADEAGVDLILVGDSLGMVVLGYESTIPVTMAEMSHHLKAVMRAQPRALVIADLPFAS
                     FQAGPEDAVHNSARFVKRGAEGVKLEGGRRVLPQIEAILAADIPVLGHLGLTPQSVHA
                     FGGYRVQARGADAAEALLEDAQALERAGVFAIVLEGIPRELGAEVSRALTIPTIGIGA
                     GAECDGQVLVIHDLVGLSFGKPARFVRRYAQVGDAIRGAVASFRDDVRAGRYPSPEET
                     YAAAPETPEKPACKS"
     gene            complement(4352..>4795)
                     /locus_tag="VFM00_02800"
     CDS             complement(4352..>4795)
                     /locus_tag="VFM00_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HET7497203.1"
                     /translation="QELRDLNQPDLFHDAVVSDYMFQKDRIFANLNLSDRELALYDKI
                     APVLERDLPMPSLVVYLQASPEVIWNRIQQRGRPYERIMDPKYTATLAEAYNYFFFHY
                     RDSPLLVVNTNEMNFVDRRADLEELVARIESHTEGVAYLSPSGNP"
BASE COUNT          690 a         1707 c         1707 g          691 t
ORIGIN      
        1 cctcgacccc ccggctccgg agctcggcca gctccccggg ggaaagccgg ctcgccccgt
       61 cggtgagcag gaccacgtcg cggctccagg tcaggagggc gtgggccagc gcctcgccgg
      121 aggcgccgtg cccgtagcag gccaggcggc ggtcgcgcca ttcccagccg tcgcagtaag
      181 gacagtggtg gacgctggtt ccgtagaagg attcgatgcc ggggatggga gggatccggt
      241 cgacgacccc ggtggcgagg aggatcttct tcgcggaaac gggacgtccc cgctggagcg
      301 aaacccggaa cgcggtgtgc ccccggcgcg tcgcgaccgg gacgatgcgg gtcacttctt
      361 cacggcggat cgagacgccg tacgcgagca cctcccggcg ccccacctcg aggagcgccg
      421 cgggaggaat gccgtcgcgg cccaagtagc cgtgcatccg gcgggacgcc gcgttgcgat
      481 agcgcccgtg atcgaagagg accacgcgcc ggcgggcccg ccccaggacc agggcggcgc
      541 tcagcccggc ggggcctccg ccgacgatga cgacgtccac ctcgcggccc ggtgccgcca
      601 cggccgcgag tcgcccctca ggacttccgg tcttcgacag ccgtctcgcg cggggtcgcg
      661 gcgggtggcg ccggcggagc gggcgcgggc ttgtcgctca cctgaagctc gttctccacc
      721 tcgcgcatcc ccttcttgaa ttcctggatg ccgcggccga ccgagcggcc gatttccggg
      781 atccgcttcg cgccgaagat caggagcacg atcaggaaca gccagaacag ctcctggggt
      841 ccgatgttga acatggttgg cgttcgcctc tccgagccgg gtcgagccgg gctccgtggg
      901 ggtcatggcg cttgcgtgaa gcgccgcgcg tcgcggccca cgatgaccgt gacgtcgatc
      961 atctcctggg aggactgctg ctggatgacg tggcgcgttc ccaggtactc cgccacgatc
     1021 ttcgccggct ccggtgcctc ggtccggttc acgaccaggg tcgtccgata atccgagcga
     1081 tccgcgttcg atacttccag tacgtcgaaa ccgccgtcgc ggaggtacga agccagctcg
     1141 cccgcgagcc cggcccgccc gcttccgttc aacagctcga tccggatcgg ctgcttcggc
     1201 cggctcgtca cctgcgggcc cacccgcagc gccaccgata cggcgcagaa ggcgatgagc
     1261 acgaacagcc ccacgatcgt gaaatcgagc gccgggctcc cggccttgcc cttggatccc
     1321 atcgcgcgtc ttccggctca gcgggcgcgg cgacgcggcc gcgtccgctc cgccggggcg
     1381 tcgaggcgct tccgcaggcg ccgatacgtc cgatcgatcg cctccggaag aaccttcgtc
     1441 tccgccagca ccggcatgaa gttcgtgtct ccgttccacc ggggcacgac gtgccagtgc
     1501 aggtggccca ggacgccggc ccccgagacg cgacccaggt tggctccgag attgaacccg
     1561 tgcgggcgat actcgcgctt cagggcatcc acgacgcgtc ccagccagag gttcacgtcg
     1621 gcggcctcct ccacgttcaa ccccgccggg gaacccaggt gcctgcgcgg tgccaccatg
     1681 acgtgcccta cattgtaggg gtacaggttc agcatgacga gcgtgagtgg ggtagacgca
     1741 aggaccagat tcctgccccc ccgcggccgg gccgcgacgt cgcagaagag gcaggatgcc
     1801 ttggctttgc gcccgggccg tttcgccggc gccacgtact ccatccgcca gccggcccag
     1861 agccgctcca tcacccctcc ccccacgcct gcagcgtctc ttccaccatc cggagctggt
     1921 cgggcgtgtt cacgccgaag cactcgcggg gatcggccgc gcgctcgatg ccgacgcggc
     1981 gtcccgcccg gctcaggatg gcaagggtgt cggtcagata gtactccttc tgggcgttgt
     2041 cggcccgaag ctgcccgagc gcctcgcgca agggctcctt ctcgaagaca tagatccccg
     2101 agttcacttc gcgcacggcc cgctcggcgg gcgtggcgtc cttctcctcg acgatcttca
     2161 cgaaggcgcc cgcggcgtcg cggacgacgc ggccgtaccc ggccgggtcc tccagctcgg
     2221 ccgagaggac cgtgacggca ttcccctccg aagcctggcg ggccagtagg cgccggagcg
     2281 actcggtccg gacgagcggc atgtcccccg cgagaatcag cacgtggggc cccccgggcg
     2341 ccgcctcgtc ccacgccacc tgtacggcgt ggccggtgcc gagctgcggc gcctgcgtca
     2401 cgaagcggat cgcgcggcct cggagcgcct cccgcaccag ctcttcgcga tgtcccacca
     2461 cgacgacgac gcgatcgggg tggagcggtg ccaggcgcgc gaggacgtgg ccgatgagcg
     2521 gcgtgccgag cgcgggatga agcaccttgg gaagatcgga gttcatgcgc tttcccatgc
     2581 cggcggcgag aaggagcgcg gtgaacggcg gcagggcgtc gggggcggac atgggcggtg
     2641 atcctagggg gtcacggcga ggttgtcgat gagcctcgtg cggccgatcc gtatggcggc
     2701 gatgaggagg gcgcggcggt ccagcctgcg gagcggctcg aaggtgcgcg ggtccaccag
     2761 cgccaggtag tccacaccat cgggagtggc ctcccggcgg agcaccgcgc gcgccgcggc
     2821 cgtgatgcgg gccgcgttcc gcaccccggc gcgcgcctgg tcgcgcccgg cgcgaagggc
     2881 gcgggagagt cccgccgccc gcgcgcgctc cccggggctc aggtaggcgt tccgcgaaga
     2941 gagcgcgagc ccgtcccgct cccggaccgt gggagcgacc cggagcgtcg ccgggctgtc
     3001 gagatcgcgc atcatcgccc cgaccagggc ggcctgctgc gcgtccttct ggcccaggat
     3061 cagcgtgtcg ggctccacga tctggaggag cttcatcacc acggtgagca cgccggtgaa
     3121 gtgccccggc cgtacgaccc cctcccatcg cgacgcgatc ggtcccggca cgacccgcgt
     3181 ctccgccccc ggcggataca gcgcgcgcac cgtggggagg aagagccagt ccacgccggc
     3241 ctcgcggcag agccgcgtgt cgcgcgcgag ggggcgcgga tagcgcgcga agtcctcgcg
     3301 cgggccgaac tggagcgggt tgacgaagat gctcgcggcg acgcggcggt gcgcgcggcg
     3361 cgcgaggcgg aggagcgtga ggtggccttc gtggagcgcg cccatcgtgg gcacgaacgc
     3421 gatcgtctcc cggcgggagc gggcgcggcg cgacagggcg cgcatcgcgt ccgcgctcct
     3481 tacgatttgc acgcgggttt ctcgggggtc tcaggcgcgg cggcgtaggt ctcctcgggg
     3541 ctcggataac ggcccgcgcg cacgtcgtcg cggaacgacg cgaccgcgcc ccggatcgcg
     3601 tcgcccacct gcgcgtagcg ccgcacgaac cgcgcgggct ttccgaacga caggccgacc
     3661 aggtcgtgga tcacgagcac ctgcccatcg cattccgcgc ccgccccgat gccgatcgtc
     3721 gggatcgtga gcgcccgcga gacctcggcg cccagctcgc ggggaatgcc ctcgagcacg
     3781 atggcgaaga cgccggcgcg ctcgagcgct tgcgcgtcct cgaggagtgc ctcggccgcg
     3841 tccgcgccgc gcgcctgcac gcggtagccg ccgaaagcgt gcaccgactg gggcgtgagc
     3901 cccaggtgcc cgagcaccgg gatgtcggcg gcgaggatcg cttcgatctg cggcagcacg
     3961 cgccgccccc cttccagctt caccccctcg gcgccgcgct tcacgaagcg cgcgctgttg
     4021 tgcaccgcgt cctcggggcc agcctggaac gaagcgaagg gaaggtccgc gatcaccagc
     4081 gcccgcggct gggcgcgcat cacggctttg agatgatggg acatctcggc catcgtgacc
     4141 ggaatcgtgg attcgtagcc caacacgacc atgccgagcg agtcccccac caggatcagg
     4201 tccacgcccg cctcgtcggc gagccgcgca gtcggatagt cgtaggcggt gacggagacg
     4261 atccgctcgc cgcgcgcctt catctcgcgg atggagcgga cggtgacctt ggggcgctcg
     4321 aaggcgcggt cgtcgcggga ggtgccggcg ctcatggatt tccgctgggg ctcaggtagg
     4381 cgaccccttc cgtgtggctc tcgatccgcg cgaccagctc ctcgagatcg gcgcggcggt
     4441 ccacgaagtt catctcgttc gtgttcacga ccaggagcgg cgaatcccga tagtggaaga
     4501 agaagtaatt gtacgcttcc gccagggtgg ccgtgtactt gggatccatg atccgctcgt
     4561 acgggcggcc gcgctgctgg atgcggttcc agatcacctc gggcgaggcc tgcaggtaga
     4621 cgaccaggct cggcatcggc aggtcgcgct ccagcaccgg cgcgatcttg tcgtagaggg
     4681 ccagctcgcg atcgctcagg ttcaggttcg cgaagatccg gtccttctgg aacatgtagt
     4741 cgctcacgac cgcgtcgtgg aacaggtcgg gctgattcag gtcgcgcagc tcctg
//
LOCUS       DASSLZ010000079         9086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_148870, whole genome shotgun sequence.
ACCESSION   DASSLZ010000079 DASSLZ010000000
VERSION     DASSLZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9086
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148870"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFM00_02805"
     CDS             complement(<1..866)
                     /locus_tag="VFM00_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET7497204.1"
                     /translation="MGEALAVHRLHFAFTITFHYLFPQLTMGLALLIVILKTLYVRGG
                     DVRYNDAARFWARIFAINFAVGVVTGIPMEFQFGTNWAEFSKNAGGVIGQSLAMEGVF
                     SFFLESSFLGLFLFGEKRLGRMGHWFAAFLVFLGSWLSGFFIIATDAWMQHPVGHEVL
                     PGGAVALKSFWAFLLNPWGLWQYWHNMNGAVLTACFVMAGVGAYYVLTSRHEEQGRLF
                     LRVAVLVGAVASIIQLFPTGDMQGQMVAKHQPTTLAAMEGLFNTEKGAPLAIIGQPDL
                     ERQTLDNPILIPR"
     gene            complement(877..2595)
                     /locus_tag="VFM00_02810"
     CDS             complement(877..2595)
                     /locus_tag="VFM00_02810"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HET7497205.1"
                     /translation="MPGYRERTDARTGSVYWEVEKRGEELLEDPFLNKGTSFTAEERR
                     ELGLDGLLPPGIATEEEQRARVYENFRRSPDEVQRYLFLAGLQDRNETLFYRLLLDHI
                     EEMMPIVYTPTVGKVCELYSHLYRRPRGIYVSTRDRGRVREALRTAGAGDVRVIVATD
                     NEAILGLGDLGAGGMPIAVGKSVLYCAAAGFHPRHVLPVSLDVGTSNESLLQDPLYLG
                     VRHERLRGDAYVSLLDEFVDAVRAEFPRALVQWEDFANRNAFQVLHRYQKKIPSFDDD
                     VQGTGAVIVAGIRSGLRRAGRRLADERVVFYGAGASGAGCALAVRAAMAADGVPESEL
                     GRRVLCLDSRGLILSDRPGLEGEKRAIAADPAVAAEWSAREGKIGLDEVVRRFRPGTL
                     VGASGQPGAFTEPIVHAMASACPRPIILPISNPTSKSEAAPADVISWTNGAAIVGTGS
                     PFPSVSWKGAAYEIGQGNNAFIFPGVGLGAVAVEARWLPDAAFAAASEALAAAAPSPP
                     GPIYPPLSRLREVSLSVAIAVARALVREGAAAAIPDEAIATRIQRLIWEPRYVPYRAA
                     RPVPAA"
     gene            complement(2677..3144)
                     /locus_tag="VFM00_02815"
     CDS             complement(2677..3144)
                     /locus_tag="VFM00_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7497206.1"
                     /translation="MTPADLIRMYSINHGALHQNLAGLTHEDSLVQPEAGNCLNWVLA
                     HIVSSRNGLFKVMGSAPYWPEELQTRYARGSAPVAAAADARPFQDLVRDFDATQERLL
                     AELSEMPEAKLQEPSMRDMNVGEWLAFLHFHEAYHIGQTGLLRRLAGKEGAIR"
     gene            complement(3141..3608)
                     /locus_tag="VFM00_02820"
     CDS             complement(3141..3608)
                     /locus_tag="VFM00_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7497207.1"
                     /translation="MRFEFLSPIHKATRQIAAHLEGPCAAAGVNTREAHLLSYLRSYE
                     PCAVGDLLRVFGQRPSTMTSLLDRLDAQGLVQRVPGTEDRRRVILRLTRKGRGTADRI
                     RECLAAFEARVRAHADPRDIQGFRAVMEAIAVATGEPAASLTKPAGVRKENRR"
     gene            3753..5576
                     /gene="typA"
                     /locus_tag="VFM00_02825"
     CDS             3753..5576
                     /gene="typA"
                     /locus_tag="VFM00_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET7497208.1"
                     /translation="MLAIERRADRRAIAIVAHVDHGKTTLVDALLKQAGTFRAGQHVE
                     ECVMDSGDLERERGITILAKNTAIRYRDTVINIVDTPGHRDFGSEVERILTMVEGILL
                     LVDASEGPLPQTRFVLKKALESGKRPIVLINKIDRADARTLEVEEEIHDLFLSLATAD
                     EHLDFPVLYGSGRLGFASSAPDASAGDLRPLLDAIMKHVPAPAIAESPFKMIVANIDY
                     SDYFGRLAIGRIHSGAITRAMELAAVRPDGSVAQRGKVGKIYLFQGIERVEVAEAHAG
                     DIVVLSGFPTIEIGQTIVDPEDLAPLAAIRVDEPTLSMEFCVNDSPFSGRSGKYLTSR
                     HLLERLERELERNVGLRMERSGDPDSFLVLGRGELSLAILAETMRREGYEFALGRPQV
                     IFRTGENGETLEPYEELIIDCAEEFTGAVISALGERRGEMQHLGRHGDRTRMEFTIPS
                     RGLLGFRTEFLTLTKGTGLLNHLFDKYGPHRGPLPNRKRGAMVAKEAGDVTAYALDQL
                     GDRGLFFVKPTDPVYAGQVVGEQVKDGDLVVQACKRKHLTNMRASTSEQGIKLTPPRD
                     LNIEQAIEWINDDELVEVTPAAIRVRKRLLDHHKRKSAAAD"
     gene            complement(6141..6719)
                     /locus_tag="VFM00_02830"
     CDS             complement(6141..6719)
                     /locus_tag="VFM00_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HET7497209.1"
                     /translation="MPPSLGLVLGTAFAAGLNVYATIATLGLLHRFGAIALPESLRPL
                     AHPLILGLAILLYVVEFVADKIPAVDHVWDVVHTFIRPPAAAILAFAAVAGVGEPWRI
                     GAALLAGTVALASHGGKASARLAVNTSPEPFSTWGVSLGEDALAVALTWLAATHPVLA
                     MALAILLTAAALWVVWILYRLAKRMAARLRTA"
     gene            complement(6795..>9086)
                     /locus_tag="VFM00_02835"
     CDS             complement(6795..>9086)
                     /locus_tag="VFM00_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HET7497210.1"
                     /translation="EVRYNAQLPVSIDAPSRMEAHAQVPLEVPLRIGGDSPGTPTISS
                     PDLPAGAAIVVTSDSSALFRWTPGWSDKGLHSIHFIVERSGVAPDTCQMTTCVDIPGE
                     VTAVLDANRLQAIVTNRGITSDWLKNGPGLFYPRGSGIPVGKSAGLLLAGVVDGQVLV
                     ARPGECCDYHPGPVENGVAPIFRPEFKNYTIREGSTTDYDWRHWPTDLGAPTDAAGSP
                     LLLGDETVWSVYNDAAAPWEPAPYSSRAGLGVEVRQTSWSLPDPGAAGSTVYQWFRIR
                     NAGTSAIHDLYAAIELDPVTWETGGGCVSYSNRDHMGCDTTTGLAYAYTRDEWRAPNA
                     VGVLAFGPEPRPTAVQDLRNGTPEGIHYQLQGLNPDGTPRHERDDPALPVTPYAYTGD
                     PLLQTGWLYPYDYGLVILSSAGPFDLAPGEEQEVRFAIVAAEGLSPSDALRRLRITAA
                     EVMAPPPPPPDRAPAADAGGPYAGFAGMPVSFDGSASSDPDGDALIYTWRFGDGAVGN
                     GVQPLHTYAAAGGYHVRLSVFDAHLGTEDSTTAQIAPVDTAAALLTASGPAVRLQSAR
                     PTIWVSLEPSGGSFSASDVDLGSVTLQRQDGVGGAIPAVPDKSPALSDQDHDGIAEVA
                     VSFRTSALRTMLGDLPPGASHVDFWVRGGLTAGGRFAAPLQLSIDAGKKGLGVAVAPN
                     PLNPTAVLTFRTTRPGPARAVLFDVAGRTVRVALDQPRVDPGYHEVAIGRDAHGRDLP
                     SGVYFYRVTTPEGSASGKLVMMK"
BASE COUNT         1480 a         3157 c         3129 g         1320 t
ORIGIN      
        1 cgcgggatca ggatcggatt gtccagcgtc tgacgctcca ggtcgggctg tccgatgatc
       61 gcgagcggcg cccccttctc ggtgttgaag agcccctcca tggcggcgag ggtggtgggc
      121 tggtgtttcg ccaccatctg cccctgcatg tcccccgtcg ggaagagctg gatgatcgac
      181 gccaccgccc cgacgagcac cgccacgcgc aggaagagcc tcccctgctc ttcatggcgg
      241 ctcgtcagca cgtagtaggc gcccaccccg gccatgacga agcacgccgt gagcaccgcg
      301 ccgttcatgt tgtgccagta ctgccagagg ccccacggat tgagcaggaa ggcccagaag
      361 ctcttcaggg ccaccgcgcc gcccgggagc acctcgtgcc cgaccgggtg ctgcatccag
      421 gcgtcggtcg cgatgatgaa gaacccggag agccacgagc ccaggaaaac caggaacgcc
      481 gcgaaccagt gtcccatccg ccccaggcgc ttctccccga acaggaagag ccccaggaac
      541 gaggactcga ggaagaagga gaacacccct tccatcgcca gcgactgtcc gatcacgccg
      601 ccggcgttct tcgagaactc cgcccagttg gtgccgaact ggaactccat cgggatgccg
      661 gtcacgacgc caacggcgaa attgatcgcg aagatgcgcg cccagaagcg cgcggcgtcg
      721 ttgtagcgga cgtcgccgcc ccgcacatac agcgtcttca agatcacgat gagcagcgcg
      781 agccccatcg tgagctgcgg gaacaggtag tggaaggtga tcgtgaaggc gaagtgaagc
      841 cggtggacgg ccagcgcctc gcccatcagc cttccctcaa gcggcgggca ccggacgggc
      901 cgcgcggtag ggcacgtagc gcggctccca gatcaggcgc tggatgcgtg tcgcgatggc
      961 ctcgtcggga atcgcggcag cggctccttc gcgcacgagc gcgcgcgcca ccgcgatcgc
     1021 caccgacagg gagacctcgc ggagccggga gagcggtgga tagatcggtc ccgggggcga
     1081 gggcgcggcg gcggccagcg cctcgctcgc cgcggcgaac gccgcgtcgg gcagccagcg
     1141 cgcctcgacg gcgaccgcgc ccagcccgac accggggaag atgaaggcgt tgttcccctg
     1201 gccgatctcg taggccgcgc ccttccagga aaccgagggg aaggggctgc ccgtgcccac
     1261 gatcgccgcg ccgttcgtcc aggagatgac gtcggcgggc gccgcttccg acttgctggt
     1321 cgggttggag atgggaagga tgatcggccg cgggcaggcg gaggccatcg cgtgcacgat
     1381 cggctcggtg aaggcgccgg gctgcccgga ggcgccgacg agagtgcccg ggcgaaagcg
     1441 ccggaccacc tcgtcgaggc cgatcttccc ctcgcgggcc gaccactccg ccgcgacggc
     1501 ggggtcggcg gcgatcgcgc gcttctcccc ttccaggccg ggccggtcgg acaggatcag
     1561 cccgcgcgag tccagacaga ggacgcgccg gccgagctcg gattcgggca cgccgtccgc
     1621 ggccatcgcc gcgcgcaccg cgagggcgca tcccgcgccc gaagcgccgg cgccgtagaa
     1681 gaccacgcgc tcgtcggcca ggcggcgccc cgcgcgcctc agtcccgacc ggatgcccgc
     1741 gacgatgacg gctccggttc cctgcacgtc gtcgtcgaac gacgggatct tcttctggta
     1801 gcggtgcagc acctggaacg cgttacggtt cgcgaagtcc tcccactgga ccagcgcgcg
     1861 cggaaactcg gcgcgcaccg cgtccacgaa ctcgtccagg agcgacacgt acgcatcgcc
     1921 ccgaagccgc tcatggcgga cgcccaggta gagtgggtcc tggagcagcg attcgttgct
     1981 ggtgcccacg tccaggctga ccggcaggac gtgccgcgga tggaagccgg cggcggcgca
     2041 atagagcacc gactttccga ccgcgatcgg catgccgccc gcgcccaggt cgcccaggcc
     2101 caggatcgcc tcgttgtcgg tggccacgat tacgcgcacg tcgccggcgc cggcggtgcg
     2161 gagcgcctcg cgcacccggc cgcggtcgcg cgtgctcacg tagatgccgc gcggcctgcg
     2221 gtagagatgg ctgtacagct cacagacctt gccgaccgtg ggcgtgtaga cgatcggcat
     2281 catctcctcg atgtggtcga ggaggagccg gtagaagagg gtctcgttcc ggtcttggag
     2341 ccccgcgagg aacaggtagc gctgcacctc gtcggggctt ctccggaagt tctcgtagac
     2401 gcgggcgcgc tgctcctctt cggtggcgat tcccggagga agaagcccgt ccaggcccag
     2461 ctcgcgccgc tcctcggcgg tgaaggaggt tcccttgttc agaaaggggt cttcgagcag
     2521 ctcctccccg cgcttctcca cttcccagta caccgatccc gtgcgggcgt cggtgcgttc
     2581 gcggtatccg ggcacgggcg gagtatacgg cgcccgccct cccggggcta tggcgcgacc
     2641 ccggtactct ggtaccgggt gcacgtccgc ctgcggctac cggatcgcgc cctccttgcc
     2701 ggccaggcgt cgaagcagac cggtctggcc gatgtggtac gcctcgtgga agtggaggaa
     2761 ggccagccac tccccgacgt tcatgtcccg catggagggt tcttgaagct tcgcctccgg
     2821 catctcggaa agctcggcca ggagccgctc ctgggtggcg tcgaaatcgc ggaccaggtc
     2881 ctggaagggc cgcgcgtcgg ccgccgcggc caccggtgcg ctcccccgcg cgtagcgcgt
     2941 ctggagctcc tcgggccagt agggcgcgct ccccatcacc ttgaagagcc cgttccgcga
     3001 cgagacgatg tgcgccagca cccagttcag gcagttgccg gcctcgggct ggaccaggga
     3061 gtcctcgtgg gtcagccccg ctagattttg gtgcagggcg ccgtggttga tggaatacat
     3121 gcggatgaga tcggcgggcg tcatcgtcgg ttctcctttc gaacgccggc gggcttcgtg
     3181 agcgaagcgg cgggctcccc cgtcgcgacg gcgatcgctt ccatcacggc gcgaaatccc
     3241 tggatgtcgc gcggatcggc atgggcgcgg acccgcgctt cgaaggcggc gaggcactcc
     3301 cggatgcggt cggccgtgcc gcggcccttc cgcgtgaggc ggaggatcac gcgccggcga
     3361 tcctcggtgc cgggaacgcg ctggaccagg ccctgggcgt cgaggcgatc gagcaggctg
     3421 gtcatggtgc tggggcgctg tccgaacacg cggaggaggt cccccaccgc gcacggctcg
     3481 tagctccgga gataggagag gaggtgcgcc tcgcgggtgt tcaccccggc cgcggcgcac
     3541 ggaccctcca ggtgcgccgc gatctggcgc gtggccttgt ggatcggcga gagaaattcg
     3601 aatcgcatgc ggccctccgg atcgtacgcg aacaaatagt acgatttcgt aataataggc
     3661 gccgggcggg ccccgcgcaa ccctaatttc tcgggtccgg atccggccgg cgtaccccag
     3721 agcccctcga atctgctatt ctctttcgcc caatgctcgc catcgaacga cgcgcggacc
     3781 gccgcgccat tgccatcgtg gcccacgtgg accatggaaa gaccaccctg gtggacgccc
     3841 tcctgaagca ggcggggacc ttccgcgcgg ggcagcacgt cgaggagtgc gtcatggact
     3901 ccggcgacct cgagcgcgag cgcgggatca cgatcctggc caagaacacg gcgatccgct
     3961 accgcgacac ggtcatcaac atcgtggata cgccggggca ccgcgatttc ggctcggagg
     4021 tggagcggat cctgaccatg gtggagggaa tcctcctcct ggtcgacgcg tctgaggggc
     4081 ccctgcccca gacccgcttc gtgctgaaga aggcgctgga gtcggggaag cgccccatcg
     4141 tgctcatcaa caagatcgac cgcgccgacg cccgcaccct cgaggtggag gaagagatcc
     4201 acgatctgtt cctctccctc gccacggccg acgaacacct cgacttcccg gtgctctacg
     4261 gatcgggacg cctcggcttc gcctcctcgg ctcccgacgc ctcggccggc gacctccggc
     4321 ctctgctcga cgccatcatg aagcacgttc cggcgccggc catcgccgag tcgccgttca
     4381 agatgatcgt cgccaacatc gactattcgg actacttcgg acgcctggcc atcgggcgga
     4441 tccattccgg cgcgatcacg cgcgccatgg agctggcggc ggtccggccc gacggatcgg
     4501 tggcccagcg gggcaaggtg ggcaagatct acctgttcca ggggatcgag cgggtggagg
     4561 tggccgaggc gcacgcgggc gacatcgtcg tcctctccgg cttccccacg atcgagatcg
     4621 gacagacgat cgtcgacccc gaggacctgg caccgctcgc ggcgatccgc gtggacgagc
     4681 cgacgctctc gatggagttc tgcgtcaacg actcgccctt ctccgggcgc tccgggaagt
     4741 acctgaccag ccggcacctc ctggagcggc tggagcgcga gctggagcgg aacgtcggcc
     4801 tccgcatgga gcgctccggc gaccccgact ccttcctggt gctggggcgc ggcgagctct
     4861 cgctcgcgat cctggccgag acgatgcggc gcgagggcta cgagttcgcc ctgggccgcc
     4921 cccaggtgat cttccgcacc ggcgagaacg gcgagacgct cgagccctac gaggagctga
     4981 tcatcgactg cgccgaggag ttcaccgggg ccgtcatttc agccctgggc gagcggcgcg
     5041 gcgagatgca gcacctgggc cggcacgggg accgcacgcg catggagttc accattccct
     5101 cgcgcgggct cctggggttc cgcacggagt tcctcacgct gaccaaggga acgggactgc
     5161 tgaaccacct gttcgacaag tacggccccc accggggccc gctccccaat cgtaagcgcg
     5221 gcgcgatggt cgccaaggag gcgggggacg tgaccgcgta cgcgctggac cagctgggcg
     5281 accgcggcct cttcttcgtg aagccgaccg acccggtcta cgccgggcag gtcgtcggcg
     5341 agcaggtgaa ggacggcgac ctggtcgtgc aggcgtgcaa gcggaagcac ctgacgaaca
     5401 tgagagcctc gacgtccgag caggggatca agctgacgcc gccgcgcgac ctgaacatcg
     5461 agcaggcgat cgagtggatc aacgacgacg agctggtcga ggtgaccccc gccgcgatcc
     5521 gggtgcggaa gcgcctgctg gaccaccaca aacggaagtc cgcggccgcc gactgattct
     5581 tggcggcaca acctccttag ggcccctcga tgggaatcag ggatttatcg tagcggtacc
     5641 tgaccccgtt ccgaatcgtt tctgcccgta ccggccgctc cacaccggcg tgaacgcccg
     5701 aggaattgac ctcgatcggc agcacgcact cgtactcgtc cttgcggtct gcgtctaccc
     5761 cgactcccat caagacccgg atctcactgc ggccaaaccg tgtggagcgc atcgtgattc
     5821 gccagaaatt gtccggagcg cccttccaga accgggagac gtgggccacg tgaaacgaat
     5881 cgcgggacac gaactcgagt ccaggcccga gtcgcacccg cgccaccgcg gcttggtctt
     5941 catgggcatt cccgccctgg agcccaatca agacttggaa aggctcccga gggccgcccg
     6001 ctcgctccaa ccggatggtg gcaacgaaac tcggggcctg atctctcggg ccccaggcaa
     6061 aggcagggga ggcgccgatg agagaccaca ccacgagaag aaacttccag tagcgatgcc
     6121 gcatcgcttg agccttggag tcaggcagtt cggagcctcg ccgccatccg cttcgccagc
     6181 cggtagagaa tccacaccac ccacagcgcc gccgccgtga gcaggatggc gagcgccatc
     6241 gccagcacgg ggtgggtcgc cgcgagccac gtgagcgcga ccgcgagcgc atcctccccc
     6301 aggctgaccc cccaggtgct gaacggctcc gggctcgtgt tgaccgcgag ccgcgccgag
     6361 gccttcccgc cgtgcgaggc gagcgcgacc gtgccggcga gaagagcggc gccgatccgc
     6421 cacggctccc ccacgccggc gaccgcggcg aaagcgagga tcgccgccgc cggcggccgg
     6481 atgaaggtgt ggaccacgtc ccacacgtgg tccacggccg ggatcttgtc cgcgacgaat
     6541 tccacgacgt agagcaggat cgcgagcccg agaatgagcg gatgcgcgag cggacgaagc
     6601 gactcgggca gcgcgatcgc cccgaagcga tgcagcaagc cgagcgtggc gatggtggcg
     6661 tagacgttga gccccgcggc gaacgcggtg ccgagcacca gtccgaggga tggaggcatg
     6721 gaatcagtgt agcgcgggac gccccgattg gaagcgcccc gcgtgcgatc cggtccggct
     6781 cacccggccg gccctcactt catcatgaca agcttccctg aggcgctccc ctccggggtg
     6841 gtcacccgat agaagtagac gcccgagggc aggtcgcgcc cgtgcgcgtc gcggccgatc
     6901 gcgacctcgt ggtagccggg atccacccgg ggctggtcca gcgcgacccg caccgtccgt
     6961 ccggccacgt cgaacagcac ggcgcgcgcc gggccgggcc tcgtcgtgcg gaacgtgagc
     7021 accgccgtcg gattcagcgg gttcggggcg accgctaccc ccaggccctt cttccccgcg
     7081 tcgatgctga gctgaagcgg cgccgcgaag cgcccgcctg ccgtcagccc tccccgcacc
     7141 cagaagtcca cgtggctcgc ccccggcggc agatccccga gcatcgtgcg cagggccgaa
     7201 gtccggaacg agaccgcgac ctccgcgatg ccgtcgtggt cctggtcgga aagcgccgga
     7261 ctcttgtccg gcacggccgg gatggcgccg cccacgccgt cctgacgctg cagcgtcacc
     7321 gaccccaggt ccacgtcgga ggccgagaag gagccgcccg acggttcgag ggagacccag
     7381 atggtgggcc gcgccgattg cagccgcacg gcgggaccgc tcgcggtgag cagcgccgcg
     7441 gccgtgtcga ccggcgcgat ctgagcggtc gtggagtcct ccgtgccaag atgcgcgtcg
     7501 aagaccgaca gtcgaacgtg atagccgccc gcggccgcat aggtatggag gggctgaacg
     7561 ccgttgccga cggctccatc gccgaagcgc cacgtataaa tgagcgcgtc gccatcggga
     7621 tcgctggatg ccgacccgtc gaacgagacc ggcatgcccg caaaccccgc gtacggtccg
     7681 cccgcgtccg ccgcgggcgc tcgatcggga ggcggcggcg gtggcgccat gacctcagcg
     7741 gcggtgatcc ggagccgccg gagggcgtcg gacggcgaga ggccctccgc ggcgacgatc
     7801 gcgaaccgca cctcctgctc ttctcccgga gcgaggtcga acggccccgc tgacgacagg
     7861 atcacgagtc cgtagtcgta ggggtacagc catcctgtct ggaggagcgg atcccccgtg
     7921 tacgcgtagg gcgtgacggg aagggctgga tcatcccgct cgtgacgcgg cgttccatcg
     7981 ggattcagtc cctggagctg ataatggatc ccctccggcg tgccatttcg cagatcttgg
     8041 accgccgtcg gtctcggttc gggaccgaag gccaggaccc cgaccgcatt cggcgcgcgc
     8101 cattcgtccc gagtgtaggc ataggcaagg ccggtcgtcg tgtcgcaacc catgtggtcg
     8161 cggttggaat aggaaacgca cccgccgccc gtctcccaag tgaccgggtc cagctcgatg
     8221 gccgcgtaca ggtcgtgaat cgcgctcgtt ccggcgttgc ggatccggaa ccactggtac
     8281 accgtgctgc ccgcggcccc gggatcgggc aagctccatg aggtttgccg cacttcgaca
     8341 cccagccccg cgcgcgacga gtaaggcgcc ggctcccagg gcgcggcagc gtcgttgtag
     8401 acgctccaca ccgtctcgtc tccgaggagc agcggcgacc cggccgcgtc ggtgggggca
     8461 ccgagatcgg tcggccagtg gcgccagtcg tagtccgtgg tcgagccctc tcgaatcgtg
     8521 tagttcttga actcggggcg gaagatcggc gcgacaccgt tctcgaccgg ccccgggtgg
     8581 tagtcgcagc attctcccgg gcgcgcgacg agaacctggc catcgacgac gccggcgagc
     8641 aggagtcccg ccgacttgcc gaccggaatc cccgagccgc gtggatagaa cagaccaggg
     8701 ccattcttca gccagtcgct cgtgattccc cggttcgtga cgatggcctg taaccggttc
     8761 gcgtccagga cggccgtcac ctcgcccgga atatccacgc aggtcgtcat ctggcaggtg
     8821 tcgggcgcga cgcccgatcg ctccacgatg aagtggatcg agtggaggcc cttgtcggac
     8881 caccccggag tccaccggaa caacgcgctg ctgtcgctcg tgacgacgat ggcagcgccg
     8941 gcgggcaggt ccggcgatga gatcgttggg gtgccgggcg aatcgcctcc gattcggagc
     9001 ggcacttcga gaggcacctg ggcgtgcgcc tccatccggc tcggcgcgtc gatcgaaacg
     9061 ggcagctggg cgttgtagcg cacctc
//
LOCUS       DASSLZ010000080         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_790501, whole genome shotgun sequence.
ACCESSION   DASSLZ010000080 DASSLZ010000000
VERSION     DASSLZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790501"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VFM00_02840"
     CDS             <1..315
                     /locus_tag="VFM00_02840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7497211.1"
                     /translation="YEYMAMGKPVVAPRLGPLADGIVDGATGILFPRRDPAALQAALA
                     SLLGDEALRARMGASARAHVLAHHTWDRNAARVLEGIGAAPGAGAAISGGRGRAGTPS
                     GT"
     gene            329..1081
                     /locus_tag="VFM00_02845"
     CDS             329..1081
                     /locus_tag="VFM00_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497212.1"
                     /translation="MKTRRWAVPALLLAACAAAFCGSREQARAQGSYIPSPPEQDSIL
                     YPEQKQDPRVAPHDAHNAYTKDPYSPKNEKKGFVNNDYFSIPVNPTAAQLIPIVENGH
                     LGSKNNPRGFWPRYNEGNKDQAFGELKYVLWVFPNHPRALHLLGMLSRETHQPAIPIA
                     YYEKALRLFPDRAYTHAQYGAYLVEIGEKTQGILQLQQALQMDPSLLTARAWLDKAKR
                     SVGMEVGDLAPGETAPAASAPTQQDGMYVPRR"
     gene            complement(1103..2611)
                     /locus_tag="VFM00_02850"
     CDS             complement(1103..2611)
                     /locus_tag="VFM00_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET7497213.1"
                     /translation="MSATAGKPSAASFASVLKHAGIYGLGNVGIRIAGLLLVPLYTYN
                     LSPAQYGVIEYLDLTALAIGMLFSLGLANAIYRYYYLAPEGERKRVVSTALLPILAVS
                     FAIAAALFLAAPRLAQGVFHDPRFTRWLRILFVGFGFNMVAEFGMTYLAVLLRSTLFS
                     TITVIKFIVGALLNVFFLVGLHWGVEGILVSNLITNLALGGVLLAYVVRSVGLSFDGA
                     LLRKMVGYGYPLVLVQLSLFGINFADRFFLQASAGFAQVGIYALAYKFGIMLNTLVVA
                     SFFQVWNAKSFEVANDPAAPSFYTRVFSYFTLGLLAAGLGMSLVIRDVIAIVAPPAYA
                     TAARLVPLVVVAYVLNGIGTYFELGMKLKSRTPLLGVIFAATCLLCLGLYAVMIPRWG
                     MFGAVLATVIAFAFKAVWVYREGQRAYPIRFEFGRVVPAALLAAGVWALRALLPPMPL
                     IASLAVGIALFLVYVGLMWGLGWIRREEREALMAWLRARWRPGTGGPAPETS"
     gene            complement(2608..>2943)
                     /locus_tag="VFM00_02855"
     CDS             complement(2608..>2943)
                     /locus_tag="VFM00_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497214.1"
                     /translation="DLAGVDLGASERQQNRAAWVDGDRDLGVFLESWSDPAVPKRRLI
                     GQLLEPKRFALFDWRDLRPYAAWLVPLARRLRGAVARRLGGTRGAPGSTGSAMPARAG
                     AGDEATRPE"
BASE COUNT          535 a         1000 c         1044 g          364 t
ORIGIN      
        1 tacgagtaca tggcgatggg aaagccggtg gtggcgccgc gcctgggccc gctcgcggac
       61 gggatcgtgg acggagcgac gggaatcctg ttcccgcgcc gcgaccccgc agcgctccag
      121 gcggcgctcg cctcactcct gggcgacgag gcgctccggg cgcgcatggg agcgagcgcc
      181 cgcgcgcacg tcctggccca tcacacctgg gaccggaacg cggcgcgggt cctggagggg
      241 atcggcgcgg ccccgggggc gggcgccgcg atctccgggg gccgcggacg cgctggtacg
      301 ccctccggaa cgtgataccc ttaagggtat gaagacgcgg cgatgggccg ttccggccct
      361 cctactggct gcctgcgcgg cggcgttctg cggctcccgg gagcaggccc gggcccaggg
      421 aagctacatt ccctcaccgc cggagcagga ctcgatcctc tacccggagc agaagcagga
      481 tccccgggtc gcgccgcacg acgcgcacaa cgcgtacacg aaggacccgt actcgccgaa
      541 gaacgagaag aaggggttcg tgaacaacga ctacttctcg atcccggtga acccgacggc
      601 cgcgcagctc atcccgatcg tcgaaaacgg gcacctcggc tcgaagaaca acccgcgcgg
      661 gttctggccg cgctacaacg agggaaacaa ggaccaggcc ttcggcgagc tcaagtacgt
      721 cctctgggtc ttcccgaacc atccccgcgc gctccacctg ctggggatgc tgtcccgtga
      781 gacccaccag cccgcgatcc cgatcgccta ctacgagaag gcgctccggc tcttcccgga
      841 ccgcgcctac acgcacgcgc aatacggggc ctacctggtc gagatcggtg agaagaccca
      901 gggaatcctg cagctgcagc aggcgctgca gatggacccc agcctgctca cggcgcgcgc
      961 ctggctggac aaggcgaagc ggtcggtcgg gatggaagtg ggggacctcg ctccggggga
     1021 aacggctccg gcggccagcg cgcctaccca gcaggacgga atgtacgtgc cgcggcgcta
     1081 ggcgccggac gtcgtttcgc cgttacgaag tctccggggc cggccccccg gttccgggac
     1141 gccaccgggc ccgcagccac gccatgagcg cctcccgttc ctcccgtcgg atccatccaa
     1201 gcccccacat cagtccgacg tacacgagga agagcgcgat ccccacggcg agggacgcga
     1261 tgagcggcat cggcgggagg agcgcgcgca gggcccagac ccctgccgcg agcagcgccg
     1321 ccggaacgac ccgcccgaat tcgaaacgga tcggataggc gcgctgtccc tcgcgataga
     1381 cccacacggc cttgaacgcg aacgcgatga ccgtggcgag cacggcgccg aacatccccc
     1441 agcgggggat catcacggcg tagaggccga ggcagaggag gcatgtcgcg gcgaagatca
     1501 cgccgaggag cggcgtgcgg ctcttgagct tcatccccag ctcgaagtag gtcccgatcc
     1561 cgttcaggac ataggccacg acgacgagcg ggacgagacg cgcggccgtc gcgtacgcgg
     1621 ggggcgccac gatggcgatc acgtcgcgga tcacgagcga catgccgagg ccggccgcga
     1681 gcaggcccag ggtgaagtag ctgaagacgc gggtgtagaa cgacggcgcc gccgggtcgt
     1741 tcgcgacctc gaacgatttc gcgttccaca cctggaagaa ggacgcgacc accagggtgt
     1801 tcagcatgat gccgaacttg taggcgaggg cgtagatgcc cacctgcgcg aagccggcgc
     1861 tggcctgcag gaagaagcgg tcggcgaagt tgatgccgaa caggctgagc tggaccagca
     1921 ccaggggata gccgtagccc accatcttcc ggaggagtgc gccgtcgaac gaaagtccca
     1981 ccgagcggac cacgtaggcg agcaggaccc ctcccagcgc cagattcgtg atcaggttgc
     2041 tgaccaggat cccttccacg ccccagtgca ggcccaccag gaagaagacg ttgaggagcg
     2101 cccctacgat gaacttgatc accgtgatcg tgctgaagag cgtggagcgc aggagcacgg
     2161 ccaggtaggt catgccgaac tcggccacca tgttgaaccc gaatccgacg aagagaatgc
     2221 ggagccagcg ggtgaagcgc gggtcgtgga agactccttg ggcgagccgg ggcgccgcga
     2281 ggaagagcgc cgccgcgatc gcgaaggaga ccgcgaggat gggaaggagc gcggtcgaga
     2341 cgacgcgctt ccgctcgccc tcgggtgcca ggtagtagta gcggtagatc gcgttcgcga
     2401 gtcccaggct gaagagcatc ccgatcgcga gcgccgtcag atcgaggtac tcgatgacgc
     2461 cgtactgcgc cggcgagagg ttgtacgtgt agagcgggac caggaggagt ccggcgatgc
     2521 ggatgccgac gttcccgagg ccgtagatgc cggcgtgctt gaggacgctc gcgaaggagg
     2581 cggcggaggg cttcccggcg gttgcgctca ctcggggcgg gtcgcctcgt cgccggcccc
     2641 ggcccgcgcc ggcatggcgc tcccggtcga gcccggcgcg ccacgcgtcc cgcccagccg
     2701 gcgcgccacc gcgccgcgca ggcgccgcgc gaggggcacg agccaggccg cgtaggggcg
     2761 gaggtcgcgc cagtcgaaga gggcgaaacg cttcggctcc agaagctgcc cgatcagccg
     2821 gcgcttcggc accgcgggat cggaccatga ttccaggaac acgcccagat cgcggtcccc
     2881 gtccacccac gcggcgcggt tttgctgccg ctccgaggcg ccgaggtcca cccccgcgag
     2941 atc
//
LOCUS       DASSLZ010000081        20726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1424271, whole genome shotgun sequence.
ACCESSION   DASSLZ010000081 DASSLZ010000000
VERSION     DASSLZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 20726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20726
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424271"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..962
                     /locus_tag="VFM00_02860"
     CDS             141..962
                     /locus_tag="VFM00_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7497215.1"
                     /translation="MAAGRFPGIPTFLGSLAAAAALVAGVGVSLGQSSSTLFQATAGD
                     GDPSAPEISTVEMKSALAAGTAVVFDARPAMEYAMGHIPGARNVAAKPGVPMSAYVSD
                     VAEIGRALGGDKSKAIILYCNGPFCGKSRRLAAELKAAGFSNVRRYQLGMPVWRALGE
                     PVQIEPDGLRRVLAEDRTAFVIDARDAGAFAGGSLPGARNIARSGVLDGKDVGALKNA
                     KNDGRLPMEDHNTRVIVVAGSAADARWVAEHIAREAFSNVAFFGGTFEEALALAK"
     gene            complement(976..2499)
                     /locus_tag="VFM00_02865"
     CDS             complement(976..2499)
                     /locus_tag="VFM00_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET7497216.1"
                     /translation="MTGGGMAGPSLAAARIGPEEVVDRLGELMIVGVPGTTMTPEVEA
                     RLRAIAPSGVIFFASNFADAGGAARYARAAHAVVGTPELPALVAADEEGGMVSQLSGF
                     WEVPPSARAVASSGGPPLVKDLAARTARRLLALGVNLDFAPVVDIHSNPKNPVIGVRS
                     FGTAPSQVTACGRAAIEGFLSAGVIPVAKHFPGHGDTSLDSHVALPRLSLTMDQLSLR
                     ELRPFEGAIQAGVPMVMTAHLLVPALDADPARTATLSHAVVTELLREKLRFNGVVVTD
                     ALEMHGATALGTHGEVAVRAIEAGVDLLLYSKLEPGPEEAVAALRQALASGRLAPDRI
                     AASIARIRALRAGSAARPAEFSMALEREARDLIPAAELERIAEGAARVLKQGAGGIPL
                     RQPVDVLEVNRPETRAGMADLLRAHGLTAHEHGPAVENWPRRIEGSALMTVAARSTVS
                     AEQEATARAWLRRYPETVTVASLNPHVTDEWPEVRTLLATFDNTPASRRALAKRLAG"
     gene            complement(2496..3455)
                     /gene="rsgA"
                     /locus_tag="VFM00_02870"
     CDS             complement(2496..3455)
                     /gene="rsgA"
                     /locus_tag="VFM00_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET7497217.1"
                     /translation="MWGPGRGGYAPAIVPTTPTSARPTVLQVYGVTALIWDGHTVREA
                     TLAPRLKADTDPSRSNALVAGDEVALEEGSPPRIVSVAPRRTRLERAATGARGRDLTQ
                     VIAANADQTVIVSSFDDPPFRPGLIDRWTLLALRGGMEPVLCLNKVDLATVEAARRVV
                     LEAHIPLETIYVSAKTDEGLETLRAQLAGRSTVFVGHSGVGKSSLLRRLIPGADAATG
                     DLSERTRKGKHTTTSSRLYPLPEGGLLIDTPGVRSVVLGRTTADEVSGVFGEIAHAPA
                     CRFRPCTHRTEPGCAVLAGLRSGAVPRAVYQRYLKLLEEVELP"
     gene            3534..4361
                     /locus_tag="VFM00_02875"
     CDS             3534..4361
                     /locus_tag="VFM00_02875"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497218.1"
                     /translation="MNANVITCPRCGERLPEGFTRCDACGAYLVPHPAGSAAPPAPGR
                     KLNVAAGPRHAPARAGSSPWVYLVVGLIVGGAVGYALRSGVGTGSSGGAPKGPADVMA
                     GAGGGEGGGMGAAPGGMGGAPGGMGAGMAGGQMPPDIAAAVQKYNSALMMDPNDLQAN
                     IGLGNLEFDSQQWDRAIEHYTKALDKDPKNADVRVDRAIAYHSMGQDAKAKEEMLRVT
                     KEHPEHRNAWLNLGVVSAATGDKPGAIKAWERYLQLEPSGTHSAAIRDELNQLKQSP"
     gene            complement(4358..5020)
                     /locus_tag="VFM00_02880"
     CDS             complement(4358..5020)
                     /locus_tag="VFM00_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HET7497219.1"
                     /translation="MSADAVALFAKTPRPGMVKTRLSPPLSPDEAAAVARVCLEESLR
                     RFPAAVPASWTLFLDGALEPWLARLAADRGVAVAGQGDGDLGARLGRAFRTLHDGGAR
                     RAVAIGADSPTLDPARIATALERLERADAVLGPARDGGYYLIGIRPGREGIFSDIPWG
                     TPDVADATRRRAAESGWTVAELPEWYDVDDAEGLRRAAADAAECPELLRVVAALDARL
                     KA"
     gene            complement(5017..5868)
                     /locus_tag="VFM00_02885"
     CDS             complement(5017..5868)
                     /locus_tag="VFM00_02885"
                     /inference="COORDINATES: protein motif:HMM:NF019546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HET7497220.1"
                     /translation="MLHRLPVHGPLDLPLTFGCGQAFRWRLDDGAWSGVVAGAEARVA
                     WETPGEVLRIETRGADPGASAWARYLRLDEDPRVHLEGAEPLRALPGFEPLLGLRLLR
                     QEPWETLASFIGSAAANIPKITAGVEGLAARYGRPIAGSARRAFPPAGTIARRRERDL
                     RAVGLGFRAPYLLHTARRLDRTPLDWNALREGPLDEARARLIELPGVGAKIADCVLLF
                     GLGRLDAYPVDRWIRRATLELAGRSRANDRELEAWSRRLGPGRGYLQQILFHLRRTGG
                     PFPPLLP"
     gene            5968..6528
                     /locus_tag="VFM00_02890"
     CDS             5968..6528
                     /locus_tag="VFM00_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497221.1"
                     /translation="MPFRRFAAAAFLSGAIAVALASSAAPARAAVGSASVRLVRLLEE
                     VPDSIPGRPGKVHGIRLHLIAGNVLLLRRGGDFIALLPIERREGSVDSLQYFYYLEGG
                     PLLWVFPGTRERAVRTVADGGDIRFHATRLLWRDGPEEGWIYFPDVPGNEGLKFSVVS
                     GRSVDQVDPRETKYWVELGTPGASGF"
     gene            6535..7254
                     /locus_tag="VFM00_02895"
     CDS             6535..7254
                     /locus_tag="VFM00_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET7497222.1"
                     /translation="MAARIREILFGNLGLKLASLVLALLLYAHVVTEQQRESILQIPL
                     TLTGLPDSLAVSGHPPERVGVKIRGKWKDLIRLGLTSPYLPLDLAHAVPGPFRSTITA
                     DDVAGKALPPELSKLVAVTEVEEPRAVDLVIEPKRQKRVRVALRFTGAPAAGYEVSGP
                     AVAVPDSVEVRGPKSVVGPLDSLSTLPIDITGEREKIQRQVEIDPGPLRLSVEPRRCL
                     VTVRFAKTAADQAPPGTPGGS"
     gene            7297..7944
                     /locus_tag="VFM00_02900"
     CDS             7297..7944
                     /locus_tag="VFM00_02900"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HET7497223.1"
                     /translation="MKRTTDPNPPGASAVDTGGPGGFPEVERRAPLAGLSLVPTKIFF
                     TKGVGSHRDELRSFELALKDAGIEKLNLVHVSSIFPPNCKIIPKDEGFKLIAPGSIAF
                     CVMARIASNEMRRLIAASIGCALPADKNRYGYLSEHHAYGQTDEVAGDFAEDMAASML
                     ASTLGVEFDENANWDEKEQVFKISGEIVRTFNVTQSAVISAKGFTTVLASAVLIF"
     gene            7986..8531
                     /locus_tag="VFM00_02905"
     CDS             7986..8531
                     /locus_tag="VFM00_02905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7497224.1"
                     /translation="MNRHPHTSPPRAAVLAALLLIPALGCAKTEHSAATVSSDSTAPP
                     AKETPMAAAPTPSNTTMPTPAHITVQHCLIGFSGSVPGKNITRSRDEAKKLAYEILER
                     AKKGEDFDALVEKYTDDSPPGIYKMSGSGVPPAQGEYSRDSMVPAFGNVGFKLQVGEI
                     GIADYDQSASPFGYHVIKRLQ"
     gene            complement(8515..9483)
                     /locus_tag="VFM00_02910"
     CDS             complement(8515..9483)
                     /locus_tag="VFM00_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497225.1"
                     /translation="MIPVPPRRRVLLFFIDGIGVGPADSTTNPLAAPQRPVLRLTETE
                     TPRSATAGAPAFARGVDASLGIPGLPQSATGQTAILTGVNAPAAMGRHVSGLPGPTLR
                     RILAESSLLKELTEQGRNATFLNAFGPRFFETEPPLRRLSATTLATLAAGVPLRNWED
                     LTEGRAVVHDLTHWRMRDYGFDLPRREPEEAGAIIAREAMRADFSLFEYFETDRAAHA
                     MDRATADRCLDDLSAALETVLAQADLRELLVLVASDHGNLEDLRVKTHTKNPAFFAAW
                     GAWDGLPEPRSLLDIAPLVREALGVARPASNRSAPPGGAAGATEDA"
     gene            9880..10158
                     /locus_tag="VFM00_02915"
     CDS             9880..10158
                     /locus_tag="VFM00_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7497226.1"
                     /translation="MTKADIVDEISERTGLTKKDVADTVDEFLEAVSRALASGKHIEI
                     RGFGTFKVKDRKSRLARNPRTGESVPVPPRRVPVFKVSKELKDIVAQI"
     gene            complement(10289..10993)
                     /locus_tag="VFM00_02920"
     CDS             complement(10289..10993)
                     /locus_tag="VFM00_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HET7497227.1"
                     /translation="MFLPFWDPTMILLIPAMILAFYAQWKVQSTYRKFSEIASASGRS
                     GREIAQLILRQNGITDVEVEEGNGFLSDHYDPLSKRVRLSPHNYSEPSISALAVSAHE
                     VGHAIQHAQGYAPLKFRTAIFPLANIGTSLSWIFIIAGLLFAPGFQVAGVSLLDVGIL
                     LFSFAVVFQLVTLPVEFDASNRALVQLNRLGLVSPAEQDGAKRVLSAAALTYVAAAAS
                     AVLQLVRLLILRDRRH"
     gene            11149..12171
                     /locus_tag="VFM00_02925"
     CDS             11149..12171
                     /locus_tag="VFM00_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HET7497228.1"
                     /translation="MRSDTVTRPTAAMRRAMAEAEVGDDVFGDDPTVRRLEEEVAALL
                     GKEAAVFVPTGCMGNEIAVATSTRRGDAILLERDSHLVYHEGQAITELLGRRFRFVHG
                     DRGRIAPEHVAHPPAGAEPIGLVSVEQTQNWGSGAIYPLARLQGVRQAARAAGLPVHM
                     DGARLWNASAATGIAPAEYAACADTVMTCFSKALGAPIGSAVSGSAAQIAEARAMRAT
                     FGGGMRQVGILAAGALHALRHHRDRLPEDHARAMRLALAIAEMPGLRIDPERVETNIV
                     IAEVERGEARLPRFMERLEAEGVKVLDFGGPGCFRAVTHLDVDDAALERAIAAMRSAA
                     GAAIAR"
     gene            12168..12896
                     /gene="rsmI"
                     /locus_tag="VFM00_02930"
     CDS             12168..12896
                     /gene="rsmI"
                     /locus_tag="VFM00_02930"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET7497229.1"
                     /translation="MSPGAEPSPGTLYLVGTPIGNVEDLSPRAQRLLGAVDVIAAEDT
                     RHTKGLLDRFGIRTAMVSYHDHNKDARTPELIARLGEGASVAIVSDAGSPGISDPAFT
                     LVRAAAAAGLDVVPVPGPSSAICALEVSGLPTDRFAFEGFLPRKPGKRRARIEELRDD
                     PRTLIFFESPYRLRAALADLAAAWGDRPASVSRELTKKFEETRRGTLRELLAWVESSP
                     PRGEFVLVVGGAPRDRGEDEEERA"
     gene            12893..13543
                     /locus_tag="VFM00_02935"
     CDS             12893..13543
                     /locus_tag="VFM00_02935"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015771660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7497230.1"
                     /translation="MNETWKTTSRSWFHPIARWLAGRGVTPDAITVAGLAFSLLAALL
                     FARGAFLAAAFVLAITGLCDMLDGDVARLRGATSPFGAFLDSTLDRLGEGALFGGLAT
                     YYFTRASGGAVWIRSSTVDGSFGDPDAQTLAVLALATLVLSLLVSYTRARAEGLGLEC
                     KVGIMERPERLLALMAGALLGHRFMPGVLGLLFLLTLVTVLQRVYHVRRLTRTGPA"
     gene            13570..14733
                     /locus_tag="VFM00_02940"
     CDS             13570..14733
                     /locus_tag="VFM00_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET7497231.1"
                     /translation="MGKIRVGIIGVGNCASSLVQGVHYYKNAKDTDFVPGLMHVNLGG
                     YHIRDIEFSAAIDIDKNKVGRDLSEAIYTWPNNTYKFDDVPRSGVPVARGMTHDGLGQ
                     YLSKIIQKAPGSTADIVRLLKETKTDVVVNYLPVGSEEATKWYVEQVLEAGCAFVNCI
                     PVFIAREKYWQRRFEQKKLPVIGDDIKSQVGATITHRVLTRLFRERGVRLERTSQLNV
                     GGNTDFLNMLERSRLESKKISKTNAVSSMLDYDLGAKNIHIGPSDYVEWLDDRKWAYI
                     RMEGRTFGDVPLNLEMKLEVWDSPNSAGVVIDAVRCAKLALDNGLSGALIEPSAYFKK
                     SPPVQFPDDQARQMVEDFIAKYGQKQARPKRAEAAKPVRAPRGKLKRAARRSA"
     gene            14754..15431
                     /gene="tmk"
                     /locus_tag="VFM00_02945"
     CDS             14754..15431
                     /gene="tmk"
                     /locus_tag="VFM00_02945"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017517626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET7497232.1"
                     /translation="MRGLLLTLEGIEGSGKSTQAAALAKDLESAGIPVLLTREPGGVP
                     VAETIRKVLLNPDSGSIQPITEVLLFMAARSEHVAQAVRPALEAGQVVICDRFTDATL
                     AYQGGGRSVPDDLIRRLNDISTGGIQPDRTYLLDLEVPLGAERAQRRSGVAGKDRIER
                     SGAEFFDAVRRRYLELAAREPERFLLVRGNESIADVTRLIREDAWSLIRSRALPSSGR
                     PNPRNPK"
     gene            15428..17059
                     /locus_tag="VFM00_02950"
     CDS             15428..17059
                     /locus_tag="VFM00_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET7497233.1"
                     /translation="MNRRQIFAVVVIVTALIAGTWAVGRVQETQDNTYSNIERFIQVL
                     TKVRDHYVEPVTSDKLMDAAIRGMLRTLDPYSQYLDKEEAQRLETTTHGAFGGIGISI
                     GIRDGWVTVISPIEGTPAWRAGIQGGDRIIKIDGTSTEGLSLDDAMKKMRGDKGTSVV
                     LTMYREGRDKPIDFTIVRDIITIKSVPYAGMLSNGVGYIRLSNFSERSREEMDAALAK
                     VEKENPKGLILDLRYNPGGLLSQAVEVSEEFAPRGKKIVYTRGRDPSQNKDFFSSSDQ
                     PHSRYPLVVLVNQYTASASEIVSGAVQDLDLGLVAGQTSFGKGLVQTVIPLTRSVKGP
                     KLKLTTAKYYTPSGRCIQKDEQLKDGALASTDDDEDSDTPALPDSMKAKRPKPEYHTE
                     MGRTVFGGGGITPDVELDEVKYPRLVEDLESKQLFFKYAVHYAAKHQQAPPKYAITPA
                     MREDFLGLLKTEKFDYDPDSLQAAQRFVDTGLRRELARRYASDEEAYHVAIEDDDQVR
                     AAAALFDKAPTLPKLLSLASEIHKNRVKNPETPVR"
     gene            17064..18203
                     /gene="dusB"
                     /locus_tag="VFM00_02955"
     CDS             17064..18203
                     /gene="dusB"
                     /locus_tag="VFM00_02955"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET7497234.1"
                     /translation="MQAGPIRIEGNVLLSPLCGVTDSPFRSLAKRHGAAMVFCEMTSS
                     DGLVRENPKTYELLEYKAEERPIGAQLCGADPEVMADAARIVEKLGFDVLDLNYGCPV
                     RKVIAREAGAAMLTDLDRLERVTSAVVNAVKLPVTAKIRIGWDKNSTNAPDVARVLER
                     SGVKWITVHARSRSEKFTGQAHWEVIREVKEAVPDLPVIGNGDVKTPEDAQRMIAETG
                     CDAVMVGRGSFGNPWLFKRAQRLLDGADPGPEPDARERIETAIEHLHAVAARKGPYAA
                     TLMRKHVAWYVRGLYDNSTLCREANHATTPEETEALLRAYLEKLETDPRAHERVPNGG
                     GGAANGHGDEANGDAGVNGGEQGEPGRSGAGLAEANECDGSLSAT"
     gene            18181..19083
                     /gene="larB"
                     /locus_tag="VFM00_02960"
     CDS             18181..19083
                     /gene="larB"
                     /locus_tag="VFM00_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET7497235.1"
                     /translation="MDPSRLRSLLASVRSGRLSPREAFDALASLPFENTASATVDHHR
                     GIRQHLPEVVFAQGKSIPQCVEIARVINSRSDRCLVTRVSAEQGEALVAEFGERAEWN
                     QLARTVVVRRGRKGSSSVKRSSRRDAASGFVLVVSAGTSDLPVAEEAAVTLEFMGVPV
                     RRVYDAGVAGIHRVLAHLDSLRSASAIVVVAGMEGALASVVGGLTPRPVVAVPTSVGY
                     GASFGGLAALLAMLNSCAAGVTVVNIDNGFGAGYAAGVIARQSRVVRSAAGASRGVRA
                     KAARKSAPAPKRATASKRKPRTKR"
     gene            19080..20261
                     /gene="larC"
                     /locus_tag="VFM00_02965"
     CDS             19080..20261
                     /gene="larC"
                     /locus_tag="VFM00_02965"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HET7497236.1"
                     /translation="MKIVYFDLVGGASGDMILGSLVAAGAPLEEIERQLRALPLDGFS
                     LGRGTAMRHGFEAFQLQVEARETKSHRHFTEIRRLLSDAKLPERVYRRALGTFERLGR
                     AEAEAHQVPLEKVHFHEVGAVDAIVDIVGTAWALELLDVTRCHASVIPQGRGLVGAAH
                     GTLPVPAPATLRLLEGLPVRMLEIEGELTTPTGAALIASLCDTVGEPVGMRLEKTGVA
                     TGTRDVAERPNVVRALLGEPLDTVGSGGVDVIECTIDDMNPQLYGHLTESLFESGAVE
                     VYLTPVQMKKGRPGVLVTAMCDPRRTQEVAQRLFGESTTLGLRVRREGRMELRRSITE
                     VVTPLGKLRVKTAVLPSGEERRVPEYEDLRRLAKESGRPLVEVMEAVRAFLGEGARAG
                     T"
     gene            20258..>20726
                     /locus_tag="VFM00_02970"
     CDS             20258..>20726
                     /locus_tag="VFM00_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7497237.1"
                     /translation="MIRLEGASFAVPDASGGAKTLLHPTDLTLEPGARHLVLGANGSG
                     KTTLLRLMAGLTAPSAGRIVLDDEALAAGSTLWPRVAVIFEEPDPQFLSDTVAGEIAF
                     GLESMALPPAETRARVAEALEEHGLTALAERDPRTLSAGEKTRTLLAAALAARP"
BASE COUNT         3254 a         7253 c         7304 g         2915 t
ORIGIN      
        1 cggctacggc caggaagaag accgcatgcg ctcccggtcc gcgggattcg cccgccacct
       61 cgtgaagccc gccgacccga gcgccctgcg gcagctgctc gagtcgcttc cggagtagcc
      121 cagccccgag gagcaacccc atggccgcgg gccgctttcc agggatccca accttcctcg
      181 gatcgctcgc ggcggcggcc gccctggtcg ccggcgttgg agtctccctc gggcagtcat
      241 cgtccacgct gttccaggcc acggccggcg acggcgaccc ctccgcgccc gagatcagca
      301 ccgtggagat gaagagcgcc ctggctgccg gcaccgccgt cgtcttcgac gcgcgtcccg
      361 cgatggagta cgccatgggg cacatccccg gggcgcgaaa cgtcgccgcc aagcccggcg
      421 tgccgatgtc cgcctacgtg tccgacgtcg ccgaaatcgg ccgcgcgctc ggcggcgaca
      481 agtccaaggc gatcatcctc tactgcaacg gccccttctg cgggaagagc cggcggctgg
      541 cggcggagct caaggcggcc ggcttctcga acgtgcgccg ctaccagctt ggcatgccgg
      601 tctggcgcgc gctgggcgag cccgtgcaga tcgagcccga tggcctgcgc cgcgtcctcg
      661 ccgaggatcg cacggcgttc gtcatcgacg cgcgggacgc cggcgcgttc gcgggcgggt
      721 cgctgcccgg cgcgcggaac atcgcgcgct cgggcgtgct cgacggcaag gacgtcggcg
      781 ccctgaaaaa cgcgaagaac gacggccggc tgcccatgga agaccacaac acgcgcgtga
      841 tcgtggtggc gggaagcgcc gccgacgcgc gctgggtggc cgagcacatc gcgcgcgaag
      901 cgttctccaa cgtggcgttc ttcgggggga ccttcgagga ggcgctcgcc ctggcgaagt
      961 agccccgggc ggggctcagc ccgcgaggcg cttggccagc gcgcggcggc tggcgggggt
     1021 gttgtcgaag gtcgcgagca gcgtgcgcac ctcgggccac tcgtcggtca cgtgcgggtt
     1081 gagcgaggcg accgtcacgg tctcggggta gcggcggagc caggcgcggg ccgtcgcctc
     1141 ctgctccgcg gacaccgtcg atcgcgcggc caccgtcatc agcgcgctcc cctcgatccg
     1201 gcggggccag ttctccacgg cgggaccatg ctcgtgcgcg gtcaagccgt gcgcccgcag
     1261 gagatcggcc atgccggcgc gggtctcggg gcggttcacc tccagcacgt ccaccggctg
     1321 gcgcagcggg atcccccccg cgccctgctt cagcacgcgc gcggccccct cggcgatccg
     1381 ctccagctcg gcggcgggaa tcagatcccg cgcctcgcgc tccaacgcca tcgagaactc
     1441 cgccgggcgc gcggcgcttc ccgcgcggag cgcgcggatc cgggcgatcg aggcggcgat
     1501 ccgatcgggc gccagacgac ccgacgcgag cgcctggcgc agtgccgcca ccgcttcctc
     1561 cggacccggc tccagcttgg aatagaggag caggtccacc cccgcctcga tcgcgcgcac
     1621 ggcgacctcg ccgtgcgtcc ccagcgccgt ggcgccgtgc atctcgagcg cgtcggtcac
     1681 gaccaccccg ttgaaccgaa gcttctcgcg aagcagttcc gtcacgaccg cgtgcgacag
     1741 ggtggccgtg cgcgcgggat cggcgtcgag cgccggcacg aggaggtgcg cggtcatgac
     1801 catcggcacg ccggcctgga tcgctccctc gaaggggcgc agctcacgca gcgagagctg
     1861 gtccatcgtg agcgaaaggc gcggcagggc cacgtgggag tcgagcgaag tgtcgccgtg
     1921 gccggggaag tgcttggcga ccgggatcac ccccgcggac aggaatcctt cgatcgccgc
     1981 gcgcccgcag gcggtgacct gcgagggcgc cgtcccgaac gagcgcacgc cgatcaccgg
     2041 attcttcgga ttggagtgga tgtccacgac gggcgcgaag tcgaggttca cgcccagggc
     2101 gagcagccgc cgcgcggtgc gcgccgcgag atccttcacc aggggaggcc cgcccgagga
     2161 ggcgaccgcg cgcgccgagg ggggcacctc ccagaagccg gacagctggc tgaccatgcc
     2221 cccctcttcg tcggccgcga cgagggcggg gagctccggc gtgcccacca cggcgtgcgc
     2281 ggcgcgcgcg tagcgcgcgg cgccccccgc gtcggcgaag ttgctcgcga agaagatcac
     2341 gccggagggg gcgatcgcgc gcaggcgcgc ctcgacctcg ggcgtcatcg tcgtgccggg
     2401 aacgccgacg atcatgagct cgcccagacg gtccacgacc tcctcggggc cgattcgagc
     2461 cgcagccagg gagggtccgg ccatgccgcc gcccgtcatg ggagctccac ctcctcgaga
     2521 agcttgagat agcgctggta caccgcccgg ggaaccgcgc ccgagcggag gccggccagc
     2581 accgcgcacc ccggctccgt gcgatgcgtg cagggacgga agcggcaggc gggcgcgtgc
     2641 gcgatctcgc cgaacacgcc ggacacctcg tccgccgtgg tgcggccgag cacgacgctc
     2701 cgcacgcccg gcgtgtcgat caggagcccg ccttccggga gcggatacag gcgcgagctg
     2761 gtcgtcgtgt gctttccctt cctcgtgcgc tcgctgaggt cgccggtggc cgcgtcggcg
     2821 ccggggatca gccggcgcaa gagtgacgac ttgcccacgc ccgagtgtcc cacgaacacc
     2881 gtggaacggc cggccagctg ggcgcgcagc gtctccagcc cctcgtccgt cttcgcgctc
     2941 acgtagatcg tctcgagcgg aatgtgcgcc tcgaggacga cgcggcgcgc ggcttccacc
     3001 gtggccaggt ccaccttgtt gagacagagc accggctcca tgccgccccg cagcgcgagc
     3061 agcgtccacc ggtcgatcag gccgggccgg aacgggggat cgtcgaacga cgagacgatc
     3121 accgtctgat cggcgttcgc cgcgatcacc tgggtcagat cccgcccgcg cgcgcccgtc
     3181 gccgcgcgct ccagccgcgt gcgcctcggc gccaccgaca cgatgcgggg cggcgagccc
     3241 tcctccaggg cgacctcgtc ccccgcgacg agcgcgttcg agcgcgacgg gtcggtatcg
     3301 gccttgaggc gaggggccag ggtcgcctcg cgaacggtgt gcccgtccca gatgagcgcg
     3361 gtgacgccgt agacttggag gaccgtcggt cgtgcggatg tcggcgtggt cggtacgatg
     3421 gcgggagcat agccgccgcg cccgggaccc cacaagcccc gaagccgcgc ccggggagcg
     3481 gtccgtgatc gcgcgttgaa tccgaacccc ggggcggcta gaatgcggct cgcatgaacg
     3541 ccaacgtgat cacctgtccc cgctgcggcg agcggctccc ggaaggattc acgcgctgcg
     3601 acgcgtgcgg ggcctacctc gtgccgcacc ccgcgggctc cgccgcaccg cccgcgccgg
     3661 gcaggaagct gaacgtcgcc gccggtccgc gacacgcgcc cgcccgcgcg ggaagctccc
     3721 cctgggtcta tctcgtggtc ggtctcatcg tcggcggcgc cgtcggctac gcgcttcgct
     3781 ccggcgtcgg gacgggatcg agcggcggcg cgcccaaggg cccggcggac gtgatggccg
     3841 gagccggcgg tggtgaaggc ggcggcatgg gcgcggctcc aggcggcatg ggcggcgcgc
     3901 cgggcggcat gggggccggc atggccggcg gacagatgcc ccccgacatc gcggccgcgg
     3961 tgcagaagta caactccgcg ctcatgatgg atccgaacga tctccaggcg aacatcggcc
     4021 tcggcaatct ggagttcgac tcccagcagt gggaccgggc gatcgagcac tacacgaagg
     4081 cgctcgacaa ggaccccaag aacgccgacg tgcgcgtgga ccgcgcgatc gcctatcaca
     4141 gcatgggaca ggacgccaag gcgaaggagg agatgctccg ggtcaccaag gagcatcccg
     4201 agcaccggaa cgcctggctc aatctcggcg tcgtgagcgc cgccacggga gacaagccgg
     4261 gcgccatcaa ggcgtgggag cgctatctcc agctcgaacc cagcggcacg cactccgcgg
     4321 cgatccgtga cgagctgaat cagctgaagc agagccccta ggctttgagc cgcgcgtcga
     4381 gcgcggcgac gacccgcaag agctcggggc attccgcggc atccgccgcc gcgcgccgca
     4441 gaccctcggc atcgtccacg tcgtaccact ccggaagctc cgccaccgtc cagcccgatt
     4501 ccgcggcgcg ccgcctcgtg gcgtccgcca cgtccggcgt gccccacgga atatccgaga
     4561 agattccttc gcgccccgga cggatgccga tcaggtagta gccgccgtcg cgcgcgggac
     4621 cgagcacggc atccgcgcgc tcgagacgct cgagcgcagt ggcgatccgg gccggatcca
     4681 gcgtggggct gtccgccccg atcgccacgg cgcgcctcgc gccgccgtca tgcagcgtcc
     4741 ggaaggcgcg gcccaggcgc gcccccaggt ccccgtcgcc ctggcccgcg acggcgacgc
     4801 cgcgatcggc cgcaagccgc gccagccagg gctcgagcgc gccgtcgaga aagagcgtcc
     4861 acgatgcggg gacggcggcg gggaagcggc gcaggctctc ttcgaggcag acgcgggcga
     4921 ccgcggcggc ctcgtccggc gaaagcggcg gcgagagccg cgtcttcacc atcccgggac
     4981 ggggcgtctt cgcaaagagc gcgaccgcgt cggcgctcac ggaagaagcg gcggaaacgg
     5041 ccctccggtc cgccgcaggt ggaagaggat ctgctgcagg tagccccggc ccgggcccag
     5101 ccgccgcgac catgcctcga gctcccggtc gttcgcgcgc gaccggcccg ccagctccag
     5161 cgtcgcgcgg cggatccagc gatcgaccgg ataggcgtcc aggcggccga ggccgaagag
     5221 gaggacgcag tcggcgatct tggcgcccac gccgggcagc tcgatcagcc gcgcgcgcgc
     5281 ctcgtcgagc ggcccctcgc ggagcgcgtt ccagtcgagc ggcgttctgt ccagacgtcg
     5341 cgccgtgtga agaaggtagg gggcgcggaa gccgagcccg acggcgcgga ggtcgcgctc
     5401 gcggcggcgc gcgatcgtgc ccgcgggcgg aaaggcgcgc cgcgccgagc ccgcgatcgg
     5461 ccggccgtag cgcgcggcca gcccctcgac gcccgccgtg atcttcggga tgttggcggc
     5521 cgcggagccg atgaaggagg ccagcgtctc ccacggttcc tggcgaagga ggcggaggcc
     5581 caacagcggc tcgaacccgg gcagggcgcg gagcggttcg gcgccttcga gatggacgcg
     5641 gggatcttcg tcgaggcgga gatagcgggc ccacgccgaa gcgccgggat cggcgccgcg
     5701 cgtctcgatc cggagtactt cgcccggtgt ttcccacgcg acgcgagcct cggccccggc
     5761 gacgaccccg gaccaagccc cgtcgtccag gcgccagcga aacgcctgcc cgcagccgaa
     5821 ggtgagcggt aggtcgaggg ggccgtgcac gggaaggcga tggagcacgc ccaagcgtgc
     5881 ggggaagcgc ccgcgcgcgt caaggaaagc ccggcggggg gagtttccgt tgacccgtcc
     5941 ggaaggcgcg tgtgagactc gaaggccatg cccttccgtc gcttcgccgc cgccgcattc
     6001 ctctcgggcg cgatcgccgt cgccctcgcg tcctccgcgg cgcccgcgcg cgcggccgtg
     6061 gggagcgcct ccgtccggct ggtgcgcctg ctggaggaag tgcccgattc cattcccggc
     6121 cggcccggca aggtccacgg catccgcctc catctcatcg ccgggaacgt gctcctgctc
     6181 cggcgcggcg gagatttcat tgccctcctg ccgatcgagc ggcgcgaggg cagcgtcgac
     6241 tcgctccagt atttctatta tctcgagggg ggcccgctcc tctgggtatt cccgggcacg
     6301 cgcgagcgcg ccgtgcgcac cgtcgccgac ggcggcgaca tccgcttcca cgccacgcga
     6361 ctgctctggc gggacggccc cgaggaggga tggatctact tccccgacgt gcccggcaac
     6421 gaggggctca agttctcggt cgtgagcggc cgaagcgtgg accaggtgga cccgcgtgag
     6481 acgaagtact gggtggagct ggggacgccc ggagcctcgg gcttctagcg gccgatggcg
     6541 gcccgcatcc gggaaatcct gttcgggaat ctcgggctga agctcgcgtc cttggtgctg
     6601 gcgcttctgc tctacgcgca cgtcgtgacc gagcagcagc gcgagtcgat cctccagatc
     6661 ccgctcaccc tgaccggcct tcccgactcg ctcgccgtca gcggccatcc gcccgagcgg
     6721 gtgggcgtga agatccgggg aaagtggaag gacctgatcc gcctgggtct caccagcccc
     6781 taccttccgc tggacctggc gcacgccgtg ccggggccct tccgctccac gatcaccgcc
     6841 gacgacgtgg cgggaaaggc gctcccgccc gagctctcca agctcgtcgc cgtgaccgag
     6901 gtggaggagc cgcgggccgt ggacctggtc atcgagccga agcggcagaa gcgcgtccgg
     6961 gtggcgctgc gcttcaccgg cgcgccggcc gcggggtacg aagtctccgg ccccgcggtc
     7021 gccgttccgg actcggtgga ggtccgcggg cccaagtcgg tcgtggggcc gctcgactcc
     7081 ctcagcacgc ttccaattga catcacggga gaacgcgaga aaatccagcg ccaggtcgaa
     7141 atcgatcctg gcccgctgcg gctctcggtc gagccgcgcc gctgcctcgt gaccgtccgc
     7201 ttcgcgaaga ccgccgccga ccaggcgccg cccggaacgc ccggcggctc ctgagcgcga
     7261 agcgccgggc acgatcacca aggagaccgc atcacgatga agcgaaccac cgatcccaac
     7321 ccgccgggcg cctcggccgt cgacaccggc ggaccgggag gattccccga agtcgagcgt
     7381 cgcgcccccc tggcgggact gtcgctcgtc ccgacgaaga tcttcttcac gaagggcgtc
     7441 ggcagccatc gcgacgagct ccggtcgttc gagctggcgc tcaaggacgc cgggatcgag
     7501 aagctgaacc tggtccacgt ctcgagcatc tttccgccga actgcaagat catcccgaag
     7561 gacgaaggct tcaagctgat cgcccccggg agcatcgcct tctgcgtcat ggcgcggatc
     7621 gcgtcgaacg agatgcgccg cctgatcgcg gcctcgatcg gctgcgcgct gcccgccgac
     7681 aagaaccgct acggctacct gagcgagcac cacgcgtacg ggcagaccga cgaggtggcg
     7741 ggcgacttcg ccgaggacat ggcggcgtcc atgctcgcct cgaccctcgg cgtcgagttc
     7801 gacgagaacg ccaactggga cgagaaggag caggtgttca agatctcggg cgaaatcgtc
     7861 cggacgttca acgtgactca gtccgctgtc atctccgcca agggattcac gacggtcctg
     7921 gcttcggcgg tcctcatttt ctgagcggcg cgtccgctcg cgttcccgtt ttcggaggct
     7981 ttggaatgaa tcgccatccg cacacgtcgc cgccgcgcgc cgccgtcctg gccgctctgc
     8041 ttctcatccc ggcgctcggc tgcgccaaga ccgagcattc ggcggcgacg gtgagcagcg
     8101 actcgaccgc cccgcccgcg aaggagactc ccatggccgc ggccccgacc ccgagcaaca
     8161 cgaccatgcc cacaccggcg cacatcaccg tgcagcactg cctcatcggc ttctcgggct
     8221 cggtcccggg gaagaacatc acccggagcc gcgacgaggc gaagaagctg gcctacgaga
     8281 ttctcgagcg cgcgaagaag ggcgaggatt tcgacgcgct cgtggagaag tacacggacg
     8341 attctcctcc ggggatctac aagatgagcg gaagcggcgt gccgccggcc cagggcgagt
     8401 attcccgcga ctcgatggtg cccgccttcg gcaacgtggg attcaagctg caggtgggtg
     8461 agatcgggat cgccgattac gatcagtcgg cgagcccgtt cggctaccac gtcatcaagc
     8521 gtcttcagta gcgccggccg cgccgcccgg cggcgcggaa cggttcgaag ccggtcgagc
     8581 cacacccaag gcctcccgga ccagcggagc gatatcgagc aggcttcgcg gctccgggag
     8641 gccgtcccat gccccccacg ccgcgaagaa ggccgggttc ttcgtgtgcg tcttcacgcg
     8701 caggtcttcg aggtttccgt ggtcgctcgc caccagcacg agcagctcgc gaagatccgc
     8761 ttgggccagc accgtctcga gcgcggcgct caggtcgtcc aggcagcggt cggccgttgc
     8821 gcggtccatc gcgtgcgcgg cgcgatcggt ctcgaagtat tcgaacagcg agaagtcggc
     8881 ccgcatcgct tcgcgggcga tgatcgcccc cgcctcctcc ggctcgcggc gcggaaggtc
     8941 gaagccgtag tcgcgcatgc gccagtgcgt gaggtcgtgc accaccgcgc gcccctccgt
     9001 caggtcctcc cagttccgga gcggcactcc cgcggccagg gtcgccagcg tcgtggccga
     9061 gaggcggcga agcggcggct cggtctcgaa gaagcgcggc ccgaacgcgt tcaggaaggt
     9121 ggcgtttcgt ccctgctcgg tcagctcctt gaggagtgag ctctcggcca ggatccgccg
     9181 cagggttggc ccgggaaggc ccgagacgtg ccgccccatc gccgccggcg cgttcacgcc
     9241 cgtgaggatc gcggtctgtc ccgtcgcgct ctgcgggagc ccggggatgc ccagcgaggc
     9301 gtccacgccg cgggcgaagg cgggagcgcc cgcggtcgcg gagcgcggcg tttcggtctc
     9361 ggtcagccgg agcacggggc gctggggcgc ggcgagcggg ttcgtggtgg agtcggcggg
     9421 gccgacgccg atcccgtcga tgaagaagag gagaacgcgc ctacgcggag gtacgggaat
     9481 cacgagtgaa aaaacgaaac gggagccggg ggagcagcgc acggtcggtg ctgggccggg
     9541 gacgcggaat cagtgaccgg cgctgcctgg ctcccgaatt cgtttgggat aactgaatta
     9601 ggcaaccgct gtgccacgcg cgatcgctcc aggagtttcc ggcaaggctt caagtggctg
     9661 gcgttcaaga ggatagcctc ggtgcgattc gcacggcaag gagccggtcc cccggaccgg
     9721 ccggcggccg tgcagcaggg agcgcgggcg ttgcattcgg ggaagaggct cggaaacccc
     9781 agcgggaaaa agccttgaca cgttctggcc ccggtctata gtccgcagcg cgaacgggcg
     9841 gcgtaggtcc gtgactggcc cgggtttagg gaggggctga tgaccaaggc agatatcgta
     9901 gacgagatct ccgagcgtac cggcctcacg aagaaagacg tggcggacac ggtcgacgag
     9961 ttcctcgaag ccgtttcccg cgctctcgct tcggggaagc acatcgagat ccgcggattc
    10021 gggaccttca aggtcaagga tcggaaatcg cgtctggcgc gaaatccccg gaccggggag
    10081 tcggtgcccg ttcctccccg gcgggtgccg gtcttcaagg tatcgaagga gctgaaggat
    10141 atcgtcgccc aaatctagtc ctgacgccgg cccggccgcc cgggccccgt cggaagcgga
    10201 cgtgaaaacc aaacgggacg cgagcgaaat cgccgcgtcc cgtttgacat tggttcggtg
    10261 gggcgtctgc cgcgcggtgg ttgcccggtc agtggcgccg gtcgcggagg atcaggagcc
    10321 gtacgagctg gagaacggcc gaggccgccg cggccacgta ggtcagtgcg gccgcgctca
    10381 atacccgctt cgcaccgtcc tgctccgcgg gcgacaccag ccccagccgg ttcagctgga
    10441 cgagcgcccg gttgctcgcg tcgaactcga cggggagcgt gaccagctgg aagaccacgg
    10501 cgaaggagaa gagcaggatc cccacgtcga gcaggctgac gccggccacc tggaagccgg
    10561 gcgcgaagag aagccccgcg atgatgaaga tccaggagag gctggtgccg atgttcgcga
    10621 gggggaagat cgccgtccga aacttgagcg gcgcatagcc ctgcgcgtgc tggatcgcgt
    10681 gccccacctc gtgcgccgag acggcgagcg ccgagatgga gggctcggaa tagttgtggg
    10741 gcgacaggcg gacccgcttg ctcagcggat cgtagtggtc cgagagaaag ccgttgccct
    10801 cctcgacttc gacgtccgtg atgccgttct gccgaaggat cagctgcgcg atctcgcggc
    10861 cgctccggcc gctcgcggaa gcgatctcgc tgaacttccg gtacgtcgac tgcaccttcc
    10921 actgcgcgta gaaggccagg atcatcgccg ggatcagcag aatcatcgtc ggatcccaga
    10981 acggcaggaa catcgatttc ctcctcggaa cggtgtggtt ggggcgcgaa cctctctccc
    11041 ctatactggg ccatccggta aaggtttcgc gctcgtgtct ttgagactag cagccgcccc
    11101 accccgattc aaccaatcga ggacgatctg acaacggctt acgtcgacct gcggagcgac
    11161 accgtgaccc gtccgaccgc cgcgatgcgc cgcgcgatgg ccgaggcgga agtgggggac
    11221 gacgtcttcg gcgacgaccc gacggtccgc cggctggaag aggaagtcgc ggcgcttctt
    11281 ggcaaggagg ccgccgtctt cgttccgacc ggctgcatgg ggaacgagat cgccgtcgcc
    11341 acctccacgc ggcggggcga cgcgatcctc ctcgagcggg acagccatct cgtctaccac
    11401 gaggggcagg cgatcaccga gctcctcggc cggcgcttcc gcttcgtgca cggcgaccgc
    11461 ggcaggatcg ctcccgagca cgtggcccat cctccggccg gggccgagcc gatcggcctc
    11521 gtctccgtgg agcagacgca gaactggggt tcgggcgcga tctatccgct ggcccgtctt
    11581 cagggcgtgc gccaggcggc gcgcgcggcc ggccttcccg tgcacatgga cggggcgcgg
    11641 ctctggaacg cgtcggcggc caccgggatc gcccccgccg agtacgcggc gtgcgcggat
    11701 acggtaatga cctgcttctc caaggcgctc ggcgccccga tcggctccgc cgtgtccggg
    11761 agcgccgcgc agatcgccga ggcgcgcgcg atgcgcgcca cgttcggcgg cggcatgcgc
    11821 caggtcggaa tcctcgccgc gggcgcgctc cacgcgctcc gccatcaccg cgaccgcctg
    11881 cccgaggacc atgcgcgggc catgcggctc gcgctggcga tcgcggaaat gccgggactc
    11941 cgtatcgatc cggagcgggt cgagacgaac atcgtcatcg cggaagtcga aaggggcgag
    12001 gcgcggcttc cgcgcttcat ggagcggctc gaagccgaag gggtgaaggt gctcgacttc
    12061 ggcggcccgg gctgtttccg cgccgtcacg cacctggacg tggacgacgc ggcgctcgag
    12121 cgggcgatcg cggccatgcg gagcgcggcg ggcgcggcga tcgcccgatg agtccgggcg
    12181 ccgagccctc gccgggcacg ctctacctgg tcgggacgcc gatcggaaac gtcgaggatc
    12241 tctcgccccg cgcgcagcgc ctgctgggcg ccgtggacgt gatcgccgcc gaggacacgc
    12301 ggcacaccaa gggactcctc gaccgcttcg gcatccggac ggcgatggtc tcctatcacg
    12361 accacaacaa ggacgcgcgc acgcccgagc tgatcgcgcg gctgggcgag ggcgcctcgg
    12421 tcgcgatcgt ctccgacgcg ggctcgcccg gaatctccga tccggccttc acgctggtgc
    12481 gcgccgcggc ggccgcgggt ctcgacgtgg ttccggttcc cggcccctcc agcgcgatct
    12541 gcgcgctcga ggtctcgggg cttccgaccg accgattcgc gttcgaggga ttcctgccac
    12601 ggaagccggg aaagcgccgg gcccggatcg aggagttgcg cgacgacccg cggaccctca
    12661 ttttcttcga gtcgccgtac cgcctgcgcg cggccctcgc cgatctggcg gcggcctggg
    12721 gcgaccggcc ggcttcggtt tcgcgcgaat tgacgaagaa gttcgaggag acgcggcgcg
    12781 gcacgctgcg cgagctgttg gcgtgggtgg agtcgtcacc gccgcgcggc gagttcgtgc
    12841 tggtggtggg cggggcgccg cgggatcgcg gggaggacga ggaggagcgc gcgtgaacga
    12901 gacctggaag accacgagcc ggtcctggtt ccatccgatc gcccgctggc tggccggccg
    12961 cggcgtcacg cccgacgcga tcacggtggc gggcctcgcc ttctcgctcc tggcggcact
    13021 cctcttcgcg cgcggcgcgt tcctcgcggc ggccttcgtc ctcgcgatca cgggactctg
    13081 cgacatgctg gacggcgacg tcgcgcggct ccgcggcgcg acgagcccgt tcggcgcctt
    13141 tctcgactcc acgctcgacc gcctgggcga gggcgcgctc ttcggagggc tggcgaccta
    13201 ctacttcacg cgcgcttcgg gcggagcggt ctggatccgc tcgagcaccg tcgacggctc
    13261 gttcggcgat ccggacgctc agaccctcgc cgtgctcgcc ctggccacgc tcgttctctc
    13321 gctgctcgtc tcctacacgc gggcgcgtgc cgagggcctg ggcctcgaat gcaaggtcgg
    13381 catcatggag cgccccgagc ggcttctggc cctgatggcg ggcgcgcttc tgggtcaccg
    13441 cttcatgccc ggcgtgctcg gattgctctt ccttttgacc ctcgtcaccg tcctgcagcg
    13501 cgtgtatcat gtgcgccgat tgacacgtac gggtccggcc tgatcccggt taccggaggc
    13561 agacaaacca tgggtaagat tcgcgtcggc atcatcggcg ttggcaactg cgcttcgtcg
    13621 ctcgtccagg gcgtgcacta ctacaagaac gccaaggaca ccgacttcgt ccccggcctc
    13681 atgcacgtga acctgggcgg ataccacatc cgcgacatcg agttcagtgc ggccatcgac
    13741 atcgacaaga acaaggtcgg ccgcgacctt tccgaggcga tctacacctg gcccaacaac
    13801 acctacaagt tcgacgacgt cccgcgcagc ggcgttccgg tcgcccgcgg catgacgcat
    13861 gacgggctgg gccagtacct ctccaagatc attcagaagg cgcccggctc cacggccgac
    13921 atcgtgcgcc tcctcaagga gaccaagacc gacgtcgtcg tgaactacct ccccgtcggg
    13981 agcgaagagg cgacgaagtg gtacgtggag caggtcctgg aggcgggctg cgcgttcgtg
    14041 aactgcatcc cggtcttcat cgcccgggag aagtattggc agcggcgctt cgagcagaag
    14101 aagctcccgg tgatcggcga cgacatcaag tcgcaggtcg gcgccaccat cacccaccgc
    14161 gtcctcacgc ggctcttccg cgagcggggc gtccggctgg agcgcaccag ccagctcaac
    14221 gtgggcggga acaccgactt cctgaacatg ctggagcgct cgcggctgga gtcgaagaag
    14281 atctccaaga ccaacgccgt ctcctccatg ctcgactacg acctgggcgc caagaacatc
    14341 cacatcgggc cgagcgacta cgtcgagtgg ctggacgacc ggaagtgggc ctacatccgg
    14401 atggaggggc gcacgttcgg ggacgtccct ctgaacctcg agatgaagct ggaggtgtgg
    14461 gactcgccca actcggcggg cgtcgtgatc gacgcggtcc gatgcgccaa gctggccctg
    14521 gacaacggcc tctcgggcgc cctgatcgag ccgagcgcct acttcaagaa gtcgcctccg
    14581 gtgcagttcc cggacgacca ggcccgtcag atggtcgagg acttcatcgc caagtacggc
    14641 cagaagcagg cgcggcccaa gcgcgcggag gccgccaagc cggttcgcgc gccccgcggc
    14701 aagctgaagc gcgccgcgcg ccggagcgcg taacccggga agcacgccgg ccgatgcggg
    14761 gactcctcct cacgctggag gggatcgagg gctcggggaa gtccacccag gccgcggccc
    14821 tcgcgaagga cctcgaatcg gccggcattc ccgtgctcct cacccgtgag ccggggggcg
    14881 ttccggtcgc cgaaaccatc cgcaaggtgc tcctgaaccc cgactcgggg tctatccagc
    14941 cgatcacgga agtgctactc tttatggcag cgcgttcgga acacgtcgcg caagctgtca
    15001 ggccggccct ggaagccggc caagttgtta tatgtgaccg ttttactgac gcgaccctgg
    15061 cataccaggg aggggggcgt tccgtccccg acgacctgat ccggcgtctg aacgatatct
    15121 ccacaggggg tatccaaccg gatcggacgt accttctcga cctcgaggtg ccgctcggcg
    15181 ccgaacgggc ccagaggagg agtggggtag cgggcaagga tcgcatcgag cgctccggcg
    15241 ccgaattctt cgatgcggtg cgtcggcggt atttggagct cgcggcaagg gaaccggagc
    15301 gtttcctgct agtgcggggt aatgagtcga tagccgacgt aacgcgtcta atccgagagg
    15361 acgcgtggtc cctgatccgc agccgggccc tcccgtcaag cgggcggccg aacccgagga
    15421 accccaaatg aatcgcaggc agatcttcgc ggtcgttgtc atcgtcacgg cgctcatcgc
    15481 cggcacctgg gccgtgggcc gggtccagga gacgcaggac aacacctact ccaacatcga
    15541 gcggttcatc caggtcctca ccaaggtccg ggatcactac gtggagccgg tcaccagcga
    15601 caagctgatg gacgcggcca tccgcgggat gctccgcacg ctcgacccct actcgcagta
    15661 tctcgacaag gaagaggccc agcgcctgga gaccacgacg cacggcgcgt tcggcgggat
    15721 cggaatctcg atcgggatcc gcgacggctg ggtcaccgtg atctcgccca tcgaaggcac
    15781 gccggcctgg cgcgccggga tccagggcgg cgaccggatc atcaagatcg acggcacctc
    15841 gaccgaaggg ctttccctcg acgacgcgat gaagaagatg cgcggcgaca agggaacgtc
    15901 ggtggtcctg accatgtatc gcgaaggtcg cgacaagccg atcgacttca cgatcgtccg
    15961 cgacatcatc accatcaaga gcgtccccta cgcgggcatg ctctccaacg gtgtgggcta
    16021 catccggctc tccaacttct ccgagcgcag ccgcgaagag atggacgccg cgctggccaa
    16081 ggtggagaag gagaacccca aggggctgat cctggacctc cgctacaacc cgggcggcct
    16141 cctctcgcag gccgtcgagg tttccgagga gttcgcgccg cgcggcaaga agatcgtcta
    16201 cacccggggg cgcgatccct cgcagaacaa ggacttcttc tcctcgtccg accagccgca
    16261 cagccgctac ccgctggtcg tgctcgtgaa ccagtacacg gcgagcgcgt cggagatcgt
    16321 ctcgggcgcc gtgcaggacc tggacctcgg cctggtggcc gggcagacct cgttcggcaa
    16381 ggggctcgtg cagacggtca ttccgctcac gcggagcgtg aagggaccca agctcaagct
    16441 gacgacggcc aagtactaca cgcccagcgg ccgctgcatc cagaaggacg agcagctcaa
    16501 ggacggcgcc ctggcgtcga cggatgacga cgaggacagc gacacgcccg cgcttcccga
    16561 ctcgatgaag gcgaagcggc cgaagccgga ataccacacc gagatgggcc gcacggtgtt
    16621 cggcggcggc ggcatcaccc ccgacgtcga gctggacgag gtgaagtacc cgcggctggt
    16681 cgaggatctc gagtcgaagc agctcttctt caagtacgcg gtccactatg ccgccaagca
    16741 tcagcaggcg ccgcccaagt acgcgatcac gccggcgatg cgcgaggact tcctcgggct
    16801 cttgaagacg gagaagttcg actacgatcc cgattcgctc caggccgcgc aacggttcgt
    16861 ggacacgggg ctccgccgcg agctggcgcg ccgttacgcc agcgacgagg aggcctatca
    16921 cgtcgcgatc gaggacgacg accaggtgcg ggcggccgcc gcgctgttcg acaaggcgcc
    16981 gacgttgccc aagctgctct cgctcgcgag cgagatccac aagaaccgcg tgaagaaccc
    17041 ggaaacgccc gttcgctgat cggatgcagg ccggcccgat ccgcatcgaa ggaaacgttc
    17101 tgctgtcgcc gctctgcggc gtgaccgact cgccgttccg tagcctcgcc aagaggcacg
    17161 gagcggcgat ggttttttgt gagatgacct cctccgacgg tctcgttcgc gaaaacccga
    17221 agacctacga gctgctggag tacaaggccg aggagcgccc gatcggagcg cagctctgcg
    17281 gcgccgatcc cgaggtcatg gccgacgcgg cgcggatcgt cgagaagctg ggcttcgacg
    17341 tgctcgacct gaactacggc tgcccggtgc gcaaggtgat cgcccgcgag gcgggcgcgg
    17401 ccatgctcac cgacctcgat cggctcgagc gggtcacgtc ggcggtggtg aacgcggtga
    17461 agctgccggt gaccgcgaag atccggatcg gctgggacaa gaactcgacc aacgcgcccg
    17521 acgtggcgcg ggtgctcgag cgctccggcg tgaagtggat cacggtgcac gcgcgttccc
    17581 gctccgagaa gttcaccggg caggcgcact gggaagtgat ccgggaggtg aaggaagcgg
    17641 tgccggacct gcccgtgatc ggaaacggcg acgtgaagac gcccgaggac gcccagcgga
    17701 tgatcgccga gaccggctgc gacgccgtga tggtggggcg ggggagcttc ggcaatccct
    17761 ggctcttcaa gcgcgcgcag cggcttctgg acggcgccga tccgggcccc gagccggacg
    17821 cgcgggagcg gatcgagacc gcgatcgagc acctccacgc cgtggccgcc cggaaggggc
    17881 cctacgccgc gaccctcatg cgcaagcacg tggcctggta cgtgcgcggc ctctacgaca
    17941 actcgaccct ctgccgcgaa gcgaaccacg ccacgacgcc ggaagagacc gaggcgctcc
    18001 tccgggcgta tctggagaag ctggagaccg atccccgggc gcacgagcgc gtgccgaacg
    18061 gcggcggcgg ggcggccaac ggtcacggcg acgaagcgaa cggcgacgcc ggcgtcaacg
    18121 gcggcgaaca gggcgagccc gggcgctcgg gcgccggcct ggccgaggcg aacgagtgcg
    18181 atggatccct ctcggctacg tagcctcctc gcgagcgtcc gcagcggtcg cctgagcccc
    18241 cgcgaagcct tcgacgcgct cgcctcactc cccttcgaga acaccgcctc ggccacggtg
    18301 gatcaccacc gcggcatccg ccagcacctg cccgaggtcg tcttcgccca gggaaagagc
    18361 atcccccagt gcgtcgagat cgcccgcgtc attaattcga gaagcgatcg ctgcctcgtc
    18421 acgcgcgtga gcgcggagca gggcgaagcg ctggtcgctg agttcgggga gcgagccgag
    18481 tggaaccagc ttgcgagaac tgtagtagtg cggcgaggcc gcaaggggtc atcgtcggtg
    18541 aagcgatcga gccggcgtga cgccgccagc ggattcgtcc ttgtcgtcag cgccggcacc
    18601 tccgatctcc ccgtcgcgga agaagccgcg gtcaccctcg aattcatggg agtccccgtc
    18661 cgccgcgtct acgacgccgg cgtcgccggc atccaccgcg tgctcgcgca tctcgattcg
    18721 ctccgctcag cgtccgccat cgtcgtcgtg gccgggatgg agggtgctct cgcgagtgtc
    18781 gtgggcgggc tgaccccgcg tccggtggtc gcggtcccca ccagcgtcgg ctacggcgct
    18841 tcgttcggtg ggctcgcagc actcctcgcg atgctgaatt cctgcgctgc aggcgtcacc
    18901 gtcgtgaaca tcgacaacgg cttcggcgcg ggctacgcgg cgggtgtgat cgcgcggcag
    18961 tcgcgcgtgg tccgaagcgc cgcgggcgca tcgcgcggcg tgcgcgcgaa agcggccaga
    19021 aagagcgctc cggccccgaa gcgcgcgacg gcctcaaagc gcaagccacg gacgaagcga
    19081 tgaagatcgt ctacttcgac ctggtcggcg gcgcctcggg cgacatgatc ctgggttccc
    19141 tggtcgcggc cggagcaccc ctcgaggaga tcgagcgaca gctgcgcgcc cttccgctcg
    19201 acggtttctc gctgggtcgc gggaccgcga tgcgccacgg cttcgaggcc ttccagctcc
    19261 aggtcgaagc gcgcgaaacg aaatcgcacc gccacttcac cgagatccgc cgcctcctct
    19321 ccgacgccaa gctccccgag cgggtctatc gccgcgcgct cggcacgttc gagcggctgg
    19381 ggcgtgccga ggccgaggca caccaggtgc cgctggagaa ggtgcacttc catgaggtcg
    19441 gtgccgtgga cgcgatcgtg gacatcgtgg gaacggcatg ggcgctcgag ctgttggacg
    19501 tgacgcgctg ccacgcctcg gtcatcccgc agggacgggg tctggtgggc gcggcgcacg
    19561 gaaccctgcc cgtgcccgcg cccgccacgc ttcggctcct ggaggggctc ccggtgcgga
    19621 tgctcgagat cgaaggggag ctcacgaccc ccacgggggc ggcgctgatc gcctcgctct
    19681 gcgacacggt cggggagccg gtggggatgc gcctcgagaa gaccggcgtg gccaccggca
    19741 cgcgcgacgt ggcggagcgc cccaatgtcg tgcgcgcgct cctcggcgag ccgctggaca
    19801 cggtggggtc tggcggcgtc gacgtgatcg agtgcaccat cgacgatatg aacccacagc
    19861 tctacggcca cctcaccgaa tcgctcttcg agtcgggcgc ggtcgaggtc tatctcacgc
    19921 ccgtccagat gaagaagggc cgccccggcg tgctcgtcac ggcgatgtgc gatccgcgcc
    19981 gcacgcagga agtcgcccag cggctcttcg gtgagtccac gacgctgggt cttcgggtgc
    20041 gtcgcgaggg acgcatggag ctccgacggt ccatcaccga ggtcgtgacc ccgctcggta
    20101 agttgcgcgt caagaccgcc gtgcttcctt cgggcgagga acgccgcgtg cccgagtacg
    20161 aggatctccg ccggctggcg aaggagagcg gccgcccgct cgtcgaggtg atggaagccg
    20221 tgcgcgcgtt cctgggcgag ggcgcccgcg ccgggacgtg atccggctcg agggcgcgtc
    20281 cttcgcggtc ccggacgcgt cgggcggcgc gaagaccctc cttcatccca ccgatctcac
    20341 gttggagccc ggagcgcgcc atctcgtcct cggcgcgaac gggtcgggca agaccacgct
    20401 cctccggctg atggccgggc tcacggcgcc ctccgcgggc cgcatcgtcc tcgatgacga
    20461 ggcgctcgcc gcgggatcca cgctctggcc gcgcgtcgcc gtgattttcg aggagcccga
    20521 tccccagttc ctctccgaca ccgtagccgg cgagatcgcg ttcggcctcg aatcgatggc
    20581 gctcccgccg gccgagacgc gcgcccgcgt ggccgaggcg ctggaggagc atggcctgac
    20641 ggcgctggcc gagcgcgacc cccgcaccct gagcgcgggg gagaagacgc gcacgctcct
    20701 cgccgccgcc ctcgcggcac gcccct
//
LOCUS       DASSLZ010000082         4231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1169652, whole genome shotgun sequence.
ACCESSION   DASSLZ010000082 DASSLZ010000000
VERSION     DASSLZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169652"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2693
                     /gene="ligD"
                     /locus_tag="VFM00_02975"
     CDS             <1..2693
                     /gene="ligD"
                     /locus_tag="VFM00_02975"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HET7497238.1"
                     /translation="KRDFRQTPEPRGRVAKRGGKLRFVIQKHAASHLHYDFRLELSGV
                     LKSWAVPKGPSLDPGDKRLAVMVEDHPLEYGGFEGTIPKGQYGGGTVLLWDRGTWEPR
                     GDAEAGLRKGHLAFTLHGKKLQGAFSLVRMGQEAKNWLLMKTQDEFARSGRSKSVVQE
                     RPESVASGKSIEEIAGAPRAKVWNSNRGASKTRTNKPTRARRASGLDPSKLLHARKAA
                     MPRDARPQFATLVPEAPEGDDWLHEIKYDGYRVLARIDRGRARLITRGGLDWTDRFTV
                     VADALQLLPVKQAILDGEIAMVLEDGTTSFQALQNQLRSGNDEALAYFVFDLLYQDGW
                     DLTQAPLEQRKEALSALIDGAGAGSVLRYSDHLEGQGARFHREACRSGLEGIVSKRKS
                     SVYTPGRTGSWLKTKCSSRQEFVIGGYTEPQGAREGIGSLLLGTNENGDGLRYRGRVG
                     TGFTRETLDELLQKLRPLEIDKAPFADRPPQKKGVHWVRPQLVAEVSFGSWTNDGRLR
                     HPSFQGLREDKPADEVVREAPRNGSTPATPRRASARTAAPAKTKRRPTPERRRPPDTP
                     PRIPPPVKEPGRRPPVADPPPRPRKRPMRDPVDTDAALAAITSPERVLYADEGITKLD
                     LARYYRAVAPHLLAHAAGRPLMLRRCPEGIDGPCFIQKHPGQGTPVGMDSVRVRERMK
                     TERHLVLRDEAGLLSLAQVGVLEIHVWGALAESLERPDRVVFDFDPAPESSWKDVVLG
                     ARRIRERIEALGLECFVKTSGGKGVHVVVPTRPGPTWDDVKDFSAALAAALTREEPDR
                     FTTHLAKARRGGRIFIDTLRNRRGATWVAPYSPRARRGAPVSMPISWEELTAALRPGT
                     FSIPRLLDRRGAARADAWDGMARVRQTITRTILREAAIAR"
     gene            2924..3073
                     /locus_tag="VFM00_02980"
     CDS             2924..3073
                     /locus_tag="VFM00_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HET7497239.1"
                     /translation="MLWTITIILFVLWLIGMVSNYTMGGLIHLLLLIAVITFLIRIIQ
                     GRRPV"
     gene            complement(3212..3394)
                     /locus_tag="VFM00_02985"
     CDS             complement(3212..3394)
                     /locus_tag="VFM00_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497240.1"
                     /translation="MMQQGEKYQCSNEDCGCQITVTKASQIEESEAQNPTCCCGEEME
                     LMETGARSGAQSSKSR"
     gene            complement(3421..3930)
                     /locus_tag="VFM00_02990"
     CDS             complement(3421..3930)
                     /locus_tag="VFM00_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7497241.1"
                     /translation="MQVDSLEKLYVDQLRDIYDAENQLVRALPKMAKAASSPELRTAF
                     EDHLEKTRGHVRRLDEIFRTLGHPSKGKSCKAMIGLIEEGDEMVKSDSEPSVKDAGLI
                     AAAQKVEHYEIASYGTLRTFAEFRGDRQAAQSLQETLDEEYEADKTLTQVAEAHINVQ
                     AADRGPERR"
     gene            complement(3964..>4231)
                     /locus_tag="VFM00_02995"
     CDS             complement(3964..>4231)
                     /locus_tag="VFM00_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET7497242.1"
                     /translation="RGMSFAVGAIVGAGIALLLAPTPGRDMRNRIGTTARKVGTGAKD
                     IIAKARGTMEGVREDARVAVDRGRDSFTQARQPVNRPGFKTHAG"
BASE COUNT          637 a         1474 c         1448 g          672 t
ORIGIN      
        1 agaagcgcga tttccgtcag acgcccgaac cccgcggacg ggtcgcgaag cgcggcggga
       61 agctccgctt cgtcatccag aagcacgccg cgtcgcatct ccactacgac ttccgcctcg
      121 agctgtccgg cgtgctgaag agctgggccg tgcccaaggg gccgagcctg gaccccggcg
      181 acaagcgcct ggcggtcatg gtcgaggatc atcccctcga gtacggcggt ttcgagggga
      241 cgatcccgaa aggtcaatac gggggcggca ccgtgctcct ctgggaccgt ggcacgtggg
      301 agccgcgcgg cgacgccgaa gccgggctcc ggaaagggca cctcgccttt acgctccacg
      361 ggaagaagct ccagggggcg ttctccctcg tgcgcatggg ccaggaggcg aagaactggc
      421 tcctgatgaa gacgcaggat gaattcgcgc gctccggccg ctcgaagagc gtggtccagg
      481 agcggcccga gagcgtggcg tcgggaaaga gcatcgagga gatcgccgga gccccgcgcg
      541 ccaaggtatg gaactccaac cgtggcgctt cgaaaacgcg cacgaacaag cccactcggg
      601 cccggcgcgc atccgggctc gacccgtcga agctcctcca cgcccgcaag gcggccatgc
      661 cgcgcgacgc gcgacctcaa tttgccacgc tcgttcccga ggcccccgag ggcgacgact
      721 ggctgcacga gatcaagtac gatggctacc gcgtgctcgc gcggatcgac cgggggcggg
      781 cgcgcctgat cacgcggggc gggctggact ggaccgaccg cttcacggtc gtggccgacg
      841 cgctccagct cctgcccgtg aagcaggcga tcctggacgg ggagatcgcg atggtgctcg
      901 aggacggcac gaccagcttc caggcgctcc agaaccagct tcgatccggg aacgacgagg
      961 cgctcgccta cttcgtgttc gatctcctgt accaggacgg atgggacctg acccaggctc
     1021 ccctggaaca gcgcaaggag gcgctgtccg cgctgatcga cggcgcgggc gccggttccg
     1081 tcctgcgcta cagcgaccac ctggaggggc agggggcgcg cttccatcgc gaggcctgcc
     1141 ggagcggtct ggaaggcatc gtgtccaagc gcaagtcgtc ggtctacacg ccggggcgca
     1201 ccggctcctg gctcaagacc aagtgctcgt cgcggcagga attcgtcatc ggcggctaca
     1261 ccgagccgca gggcgctcgc gaggggatcg gctccctcct gctgggtacg aacgaaaacg
     1321 gcgacgggct tcgctatcgc ggtcgcgtcg gcaccggatt cacgcgcgag accctggacg
     1381 aactcttgca gaagctgcgc ccgctcgaga tcgacaaggc tccgttcgcc gaccgcccgc
     1441 cgcagaagaa gggggtgcac tgggtgcggc cccagctggt cgccgaggtg tcgttcgggt
     1501 cctggacgaa cgatgggcgg ctccggcatc catcgtttca gggattgcgc gaagacaagc
     1561 ccgcggacga ggtcgtgcgc gaggcgcccc ggaacggatc cacccccgca acaccccgcc
     1621 gcgcttccgc tcgcaccgcg gcaccggcga agacgaaacg gcggcccacg cccgagaggc
     1681 gacggccccc cgacacgccg ccgcggatcc caccccccgt gaaggagcct ggccgccggc
     1741 ctcccgtggc cgacccgccg ccgcggccga gaaagcgccc gatgcgggac ccggtcgaca
     1801 cggacgcggc gctcgcggcc atcaccagcc ccgagcgggt gctctatgcc gacgagggga
     1861 tcaccaagct cgatctggcg cgctattacc gggcggtggc gccgcacctc ttggcgcacg
     1921 ccgcgggccg gcctctgatg ctgcggcgat gccccgaggg aatcgacggg ccctgcttca
     1981 tccagaagca tccggggcag ggaacgccgg tcggaatgga cagcgtgcgc gtgcgcgagc
     2041 ggatgaagac ggagcgccac ctggtcctgc gcgacgaggc gggtcttctc tcgctcgccc
     2101 aggtcggcgt tctcgagatc cacgtctggg gcgcgctcgc ggaatccctg gagcgtcccg
     2161 atcgcgtggt cttcgacttc gaccccgctc ccgagtcctc ctggaaggac gtggtcctgg
     2221 gcgcccggcg cattcgcgag cggatcgagg cgctcggcct ggagtgcttc gtgaagacgt
     2281 cgggagggaa gggggtccat gtggtggttc cgacacggcc cggcccgacc tgggacgacg
     2341 tgaaggactt ctcggccgcg ctcgcggccg ccctgacgcg cgaagagccg gatcggttca
     2401 cgacccacct cgccaaagcc cgccgcggcg gccgcatctt catcgacacg ctgcggaacc
     2461 ggcggggcgc gacgtgggtc gcgccctact ccccccgcgc ccgtcgcggc gcgcccgtct
     2521 cgatgccgat ctcgtgggaa gagctgaccg cggcactccg ccccggcacc ttctcgatcc
     2581 cgcgcctcct ggaccgccgc ggcgccgctc gggcggacgc ctgggacggg atggcccgcg
     2641 tacggcagac gatcacgcgc acgatcctcc gcgaggccgc catcgcgcgt tgaaagcgct
     2701 acaaagctgc ggctcctcag tacacgtaat tacacctaga agcctgcggc agcgctcctt
     2761 gcggccgatg gccacccgct ctcccttcac attggccgtt ctgggacact ggcacggatg
     2821 ctgcggtggg ggagaagcag gcgccgcttc tgctccgtcc ctcgcgtccg gtcggggcgc
     2881 ggtccaagcg ggcctcgtgg cctggacgct aaggaggttc caaatgcttt ggaccatcac
     2941 gatcatcctg ttcgtgctgt ggctcatcgg gatggtgtcg aactacacga tgggtgggtt
     3001 gatccacctg ttgctgctga tcgccgtcat cacgttcttg atccggatta tccagggacg
     3061 gcgcccggtc tgatcacggc ttggacgcgg ggcagcgcgc gaaggggcgc gcggccccgt
     3121 cgtcttgtct ggcggatcct gtctgcggac aagcttcggg cggcctgcgc gcatgcgccg
     3181 gccgcccgtg ttcgcgaggg ggtgactcgc gctagcggga cttcgagctc tgcgccccgc
     3241 tacgggcccc cgtttccatc agctccatct cctcgccgca gcagcacgtc gggttctggg
     3301 cttcggactc ctcgatctgc gacgccttcg tcacggtgat ctggcaaccg cagtcctcgt
     3361 tcgagcactg atacttctct ccctgctgca tcatgaacgc gctcctttct ggtcgccggg
     3421 ctaccggcgc tccgggccac gatccgcggc ctgaacgttg atgtgtgcct ccgcgacctg
     3481 agtcagggtc ttgtcggcct cgtactcctc gtcgagggtc tcctgaaggg attgggccgc
     3541 ctggcggtcg ccgcggaact cggcgaaggt gcggagcgtg ccgtacgaag cgatctcgta
     3601 gtgctccacc ttctgggccg ccgcgatcag gccggcgtcc ttgacgctcg gttcggaatc
     3661 gctcttcacc atctcgtcac cctcttcgat cagaccgatc atggccttgc acgatttccc
     3721 cttcgagggg tgccccaggg tgcggaagat ctcgtcgagc cggcgcacgt ggccgcgcgt
     3781 cttctcgaga tggtcttcga acgccgttcg aagctcggga gacgacgcag ccttggccat
     3841 cttcgggagg gcgcgcacga gctggttctc cgcgtcgtag atgtcccgaa gctggtccac
     3901 atagagtttt tccaacgaat cgacttgcat gacgcgctcc ttgcgcggcc ggctccgcga
     3961 gggtcagccg gcgtgcgtct tgaagcccgg ccggttcacc ggctggcggg cctgggtgaa
     4021 cgaatcccgc ccgcgatcca cggcgacgcg cgcgtcctcg cgcacgccct ccatggtgcc
     4081 gcgggccttg gcgatgatgt ccttggctcc tgtgcctacc ttgcgcgccg tagtgccgat
     4141 gcggttccgc atgtcccggc cgggcgtggg ggcgagaaga agggcgattc cggcgccgac
     4201 gatcgcgccg acggcaaacg acattccgcg c
//
LOCUS       DASSLZ010000083         7101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1297411, whole genome shotgun sequence.
ACCESSION   DASSLZ010000083 DASSLZ010000000
VERSION     DASSLZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297411"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFM00_03000"
     CDS             <1..464
                     /locus_tag="VFM00_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET7497243.1"
                     /translation="LAARLVERGADPSRTALSLFVRKSPASLRVLGLALASLESRAAG
                     KVSVLTISRAMFDEAKAAPEDADGIVQYAQSVNGALIGVLIQETAPGEVRISFRSDGS
                     VDVNDIAGRFGGGGHRNAAGARLRGELSVVRESVLQALEQAVNGGPPAPRG"
     gene            436..1410
                     /gene="truB"
                     /locus_tag="VFM00_03005"
     CDS             436..1410
                     /gene="truB"
                     /locus_tag="VFM00_03005"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET7497244.1"
                     /translation="MADLLHLVDKPEGWTSHDVVARLRGILGERRIGHAGTLDPFASG
                     LLVLGEGRTTPLLSCIGLLPKRYVARALLGVETDTQDRTGRVVASANPESIPGRDRVE
                     EALAAFRGRIMQCPPAYSAVKVGGERSYRVARRGGDPERKPRPAHVYALELSGEARHP
                     EIAFTATVSRGTYVRTLAHDLGRALGCGAHLTALRRTAIGPFQVEDALSPAREAGHAA
                     ERFRERAIPPERALSFLPRMRLDRSEADRLRFGAAPSSDPARLEAAQEDDPLPPGEAG
                     WPVALLDDEGMLVALARPMESQRPGEPMVLLRVMSATPERTPAGEEGR"
     gene            1407..2366
                     /gene="ribF"
                     /locus_tag="VFM00_03010"
     CDS             1407..2366
                     /gene="ribF"
                     /locus_tag="VFM00_03010"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HET7497245.1"
                     /translation="MTAAVPSETREGVPVAGRPIALTIGVFDGLHRGHRRVIEATVAA
                     AREMAGAAWVATFDPHPDTVVRGVEPRAWITPPEERKALLHALGVDRVEVIRFDHAVQ
                     ELLPEGFLDRILGPGAPLRTLVVGPDFRMGKNRVGDRAYLEELGKRRGFSVREAPFLM
                     GNGAKLSSTLLRREIETGLVEEAAAILGRPYALEGTVGSGAGRGTGLGYPTANLEVSP
                     QKLLPASGIYLSENELEGRIQPGITYVGSAGTFGPGPVRVEVHLLDFAGSLRGKALRT
                     LLIRRLRADKVFDSPKALIEAMDKDLADARAWWGSARGAGSRG"
     gene            2447..2716
                     /gene="rpsO"
                     /locus_tag="VFM00_03015"
     CDS             2447..2716
                     /gene="rpsO"
                     /locus_tag="VFM00_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET7497246.1"
                     /translation="MPLSPERKREVIGQFKTHEADSGSAEVQIALLTERIKYLTEHFK
                     VHKRDHHSRRGLLRMVGQRRRLLDYLKATKVERYRAVVKELGLRK"
     gene            2885..5080
                     /gene="pnp"
                     /locus_tag="VFM00_03020"
     CDS             2885..5080
                     /gene="pnp"
                     /locus_tag="VFM00_03020"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET7497247.1"
                     /translation="MPESVSLELGGRTYRIEVGKVAKQASGSTWVQFGETVVLCAAVA
                     SKDPIEKDFFPLSVEYREKTYAAGRIPGGFFKREGRPTEKEILSSRLIDRPLRPLFPD
                     GYRNEIQIMCTVLSSDQQNDSDILGVTGASAALMVSDIPFPEPVSAVRVGLVEGKMVL
                     NPTFAELDESTLDLVVAATDSNVVMVEAGAREVQESQIVEALEFAHEAIRKLNRMQHD
                     LRERVGKPKRVFPLPQRDETLAKQIAAEYGDRARQANRTAEKHARQDALDKLRDDAVA
                     KYEASNPGSGKLVRQVLNELEEHDVRHMIVKEHKRPDGRGFDDIRAITCEVSPLPRTH
                     GSSLFTRGQTQALVVATLGTKVDEQRVEELEGQSWKSFMLHYNFPPYSVGEVRPFRGP
                     GRREIGHGALAERALEPLIPSDVDFPYTIRIVSDILESNGSSSMATICGGSLSLMDAG
                     VPIKSHVAGVAMGLVGEENEFAILTDIQGVEDHLGDMDFKVAGTKDGITALQMDIKIA
                     GVSYALMAEALERAKKARLFVLSKMDAAIAAPRSELSPYAPRIYIMMIDPDKIREVIG
                     PGGKMIKKISAETGTQIDIEDTGEVRIAAFSGADGDRAREIIRGITEDPEIGRVYQGI
                     VRRVVPFGAFVEISPGKDGLVHISELEPKRVERVEDVINEGDTVLVKVIGIDREGKIK
                     LSRKQALPGYVPSEEDGASDRGGDGRRGGGGGRGGDRGPRGRRPEPSRR"
     gene            5190..6476
                     /locus_tag="VFM00_03025"
     CDS             5190..6476
                     /locus_tag="VFM00_03025"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7497248.1"
                     /translation="MSDTGRFHRTLLPSGLTVIGEEMDSVRSLAIGVWMKVGARHEDA
                     ATSGMSHFLEHMVFKGSRRRDAYQIALSLESVGGHLDAFTGRESTCFYARALEDHLDL
                     AVDVLADLSLSPRLDPADVAKEKQVVAEEIHNYDDTPDDCVHDLFADVVWNGHPLGNR
                     ILGSEESVTAFTPEEVAAYHGRHYTAGNMIVAIAGRFDWPRVVDLVERHFAPAPAGAP
                     PAPPRAANGAGRDVVHHVRDLAQQYLCIGAPGLRQEDPDRYALVLLSTVLGGGMSSRL
                     FQRVREQEGLAYSVYTYSDSYEDAGIFCAAMSVHPSQGRKAVRLTLEEFDRIIEAGIT
                     PEELSSAKEQLKGSLLLGLESTSNRMHRIARSILYSGRFLPVDELVRTIDRITAEEVR
                     EMAVRVLDRDRLSLVALGADQNGAFSEADLNHREVA"
     gene            6473..6976
                     /gene="dut"
                     /locus_tag="VFM00_03030"
     CDS             6473..6976
                     /gene="dut"
                     /locus_tag="VFM00_03030"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET7497249.1"
                     /translation="MTADARESQAVERRETRAPEAAEIGVRVTLLPHAAGAPAYETEH
                     AAGMDLRAAIEGELVLAPGHVAPVPTGIRIEIPPGYEGQVRGRSGLALRHAVGVPNAP
                     GTIDADYRGEVTVLLVNWGTEPYTVRRGDRIAQLVIAAVARARLEIAESLTETARGAG
                     GFGHTGR"
BASE COUNT         1093 a         2460 c         2555 g          993 t
ORIGIN      
        1 gcctggcggc ccgcctggtc gagcgcggcg ccgatccctc gcgcacggcg ctctccctgt
       61 tcgtccggaa gtcgccggcc agcctccgcg tgctgggtct ggccctcgct tccctggaat
      121 cgcgcgcggc ggggaaggtg agcgtgctca ccatctcccg cgccatgttc gacgaggcga
      181 aagccgcccc cgaggacgcg gacggcatcg tccagtacgc gcagtcggtg aacggcgctt
      241 tgatcggcgt gctgatccag gagaccgcgc ccggcgaggt gcggatctcc ttccgctccg
      301 acggcagcgt ggacgtgaac gacatcgccg gccgcttcgg cggtggcgga catcggaacg
      361 cggcgggcgc ccggctccgc ggcgagcttt cggtcgtgcg cgagtcggtg ctccaggcac
      421 tcgaacaggc ggtgaatggc ggacctcctg cacctcgtgg ataagcccga gggctggacg
      481 tcgcacgacg tggtcgcccg gctgcgcggc atcctgggcg agcgccggat cgggcacgcc
      541 ggcaccctcg atcccttcgc gagcggcctc ctggtcctgg gggaggggcg caccacgccg
      601 ctgctttcct gcatcggcct cctcccgaag cgctacgtcg cgcgcgcgct cctgggcgtc
      661 gagaccgaca cgcaggaccg caccggccgc gtcgtggcca gcgccaatcc ggaatccatt
      721 cccgggcgcg atcgcgtcga ggaggcgctg gccgcgttcc ggggccgcat catgcagtgt
      781 ccgccggcct actccgccgt caaggtgggc ggcgagcgct cctaccgcgt ggcgcgccgc
      841 gggggcgacc ccgagcggaa gccgcgtccg gcgcacgtct acgcgctgga gctgtccggc
      901 gaggcgcggc atcccgagat cgcgttcacg gccacggtca gccgcggcac gtacgtgcgc
      961 acgctggcgc acgacctggg gcgggcgctg ggctgcggcg cccacctgac ggcgctccgc
     1021 cgcacggcga tcggtccctt ccaggtcgag gacgcgctct cgcccgcgcg cgaggcgggt
     1081 catgccgccg agcgtttccg cgagcgcgcc atcccgcccg agcgggccct ttccttcctg
     1141 ccgcggatgc ggctcgaccg gagcgaggcg gaccggctcc gctttggcgc ggcaccatcc
     1201 tccgatcccg cgcggctcga agcggcgcag gaggacgacc cgctgccgcc cggagaggcg
     1261 ggatggccgg tggcgcttct ggacgacgag gggatgctgg tcgcgctcgc gcggccgatg
     1321 gaatcccagc gtcccggaga gccgatggtg ctcctgcgcg tgatgagcgc gacgccggag
     1381 cggacccccg ccggcgagga ggggcggtga ccgccgcggt cccttccgag acgcgcgagg
     1441 gcgtcccggt cgccgggcgt cccatcgcgc tcacgatcgg cgtcttcgat gggcttcacc
     1501 gggggcaccg gcgggtgatc gaggccaccg tcgcggcggc ccgcgagatg gcgggcgccg
     1561 cgtgggtggc caccttcgat ccgcaccccg acacggtcgt gcgcggcgtc gagccgcgcg
     1621 cctggatcac ccctcccgag gagcggaagg cgctgctgca cgcgctgggg gtggaccggg
     1681 tcgaggtgat ccgcttcgac cacgccgtcc aggagctcct ccccgagggg ttcctcgacc
     1741 ggatcctggg tcccggcgcg ccgcttcgga cgctggtcgt gggacccgac ttccggatgg
     1801 ggaagaaccg cgtgggtgac cgcgcctatc tcgaagagct gggaaagcgc cgcggcttca
     1861 gcgtgcgcga ggcgccgttc ctcatgggca acggggccaa gctctcgagc acgctgctcc
     1921 ggcgggagat cgagaccgga ctggtggagg aggcggccgc catcctgggc cggccctacg
     1981 cgctcgaagg gaccgtgggg agcggcgccg gccggggcac cggcctgggg tacccgacgg
     2041 cgaatctcga ggtctccccc cagaagctcc ttcccgcgag cggtatctac ctgtcggaaa
     2101 acgagctgga agggcggatc cagcccggga tcacctacgt cgggagcgcc ggcaccttcg
     2161 gtcccggccc ggtgcgcgtg gaggtgcacc tcctcgactt cgccgggagc ctgcggggga
     2221 aggcgcttcg gacgctattg atccggcgac tccgcgccga taaggtgttc gactcgccga
     2281 aggcgctcat cgaggcgatg gacaaggact tggccgatgc ccgggcctgg tggggaagcg
     2341 cccgcggagc gggaagccgc ggctgaaacg gaagtgcttg caacctgaac gcactgtgat
     2401 acattcccgc tccagagaga gagggaaagt caaggaggtg gcaccggtgc cgttatcgcc
     2461 tgaacggaag cgcgaagtga tcggacagtt caagacccac gaggccgact ccgggtcggc
     2521 cgaagtccag atcgcgcttc tcacggaacg aatcaagtac ctgacggagc acttcaaggt
     2581 ccacaagcgc gaccatcaca gtcgccgcgg actactccgg atggtgggtc aaaggcgacg
     2641 gctactcgac tatctgaagg cgacgaaagt cgagcgctac cgggcagtgg tcaaggagct
     2701 gggcctccgc aagtaggctc ctccccgcgc cccgaacggg ccgcagggac cccgccgcag
     2761 ttcctcccga agacaccctt acgagccctc gaaggcatga gccgtcgagg gggcgccgca
     2821 tttggcccat ggttcgccgg cggcgtcgtg tccttcgagc cagtcgctac aaaaggagat
     2881 gagaatgccc gaatcagtaa gccttgagct tggcggccgc acgtaccgca tcgaggtcgg
     2941 caaggtggcc aagcaggctt cgggttccac gtgggtccag ttcggcgaga ccgtggtgct
     3001 ctgcgccgcg gtcgccagca aggacccgat cgagaaggac ttcttcccgc tcagcgtcga
     3061 gtaccgggag aagacctacg ccgcggggcg catccccggc ggcttcttca agcgtgaggg
     3121 gaggccgacg gagaaggaga tcctctccag ccgcctcatc gaccgcccgc tccgtccgct
     3181 cttcccggac ggctatcgca acgagatcca gatcatgtgc acggtcctct cctcggacca
     3241 gcagaacgac tccgacatcc tcggcgtcac cggcgcgtcg gccgcgctca tggtctccga
     3301 cattcctttc cccgagccgg tctccgcggt ccgcgtgggc ctggtcgagg ggaagatggt
     3361 cctgaacccc accttcgccg agctggacga atccacgctc gacctggtgg tcgcggccac
     3421 cgactccaac gtcgtgatgg tggaggcggg cgcccgtgag gtgcaggaga gccagatcgt
     3481 cgaggcgctg gagttcgcgc acgaggcgat ccggaagctg aaccgcatgc agcacgacct
     3541 gcgcgagcgg gtcggtaagc ccaagcgcgt cttcccgctc ccgcagcgcg acgagacgct
     3601 ggccaagcag atcgccgccg agtatggcga ccgcgcccgc caggcgaacc gcaccgccga
     3661 gaagcacgcg cgccaggatg ccctggacaa gctccgcgac gacgcggtcg cgaagtacga
     3721 ggcgtcgaac cccggctccg gcaagctggt gcgccaggtg ctgaacgagc tggaggagca
     3781 cgacgtccgc cacatgatcg tcaaggagca caagcgtccg gacgggcgcg gcttcgacga
     3841 catccgcgcc atcacctgtg aggtcagccc gcttccgcgc acgcacggct cgtcgctctt
     3901 cacgcgcggc cagacgcagg cgctcgtggt cgccacgctg ggcaccaagg tggacgagca
     3961 gcgggtcgag gagctggaag gccagtcgtg gaagagcttc atgctccact acaatttccc
     4021 gccctacagc gtcggcgagg tccgtccctt ccgcggcccc ggccgccggg agatcggcca
     4081 cggcgcgctc gccgagcgcg ctctcgagcc gctgattccg agcgacgtgg acttccccta
     4141 caccatccgc atcgtctccg acatcctgga gtcgaacggc agctcgtcca tggcgacgat
     4201 ctgcggcgga agcctctcct tgatggacgc gggcgtgccg atcaagtcgc acgtcgccgg
     4261 cgtggcgatg gggctggtcg gggaggagaa cgagttcgcc atcctgaccg acatccaggg
     4321 ggtggaagac cacctgggcg acatggactt caaggtcgcg ggcaccaagg acggcatcac
     4381 ggcgctccag atggacatca agatcgccgg cgtctcctat gcgctcatgg ccgaggcgct
     4441 ggagcgggcc aagaaggcgc gcctgttcgt gctcagtaag atggacgccg cgatcgcggc
     4501 gccgcgctcg gagctgtcgc cgtacgcgcc gcgcatctac atcatgatga tcgaccccga
     4561 caagatccgc gaggtcatcg gaccgggcgg caagatgatc aagaagatca gcgccgagac
     4621 cggcacgcag atcgacatcg aggacaccgg cgaggtccgg atcgcggcct tcagcggcgc
     4681 cgacggcgac cgcgcgcggg agatcatccg cggcatcacc gaggatccgg agatcggccg
     4741 cgtctaccag gggatcgtgc ggcgggtggt gccgttcggc gccttcgtcg agatctcgcc
     4801 cggcaaggac ggtctggttc acatctccga gctcgagccg aagcgggtcg agcgggtcga
     4861 ggacgtcatc aacgaggggg acaccgtcct cgtcaaggtg atcggcatcg accgcgaggg
     4921 gaagatcaag ctgagccgca agcaggccct tccgggctac gtgccctccg aagaggacgg
     4981 cgcgtccgac cggggcggcg acggccgcag gggcggcggc ggcggacgag gcggcgaccg
     5041 tggtccgcgc ggacggcggc cggagccgtc gcggcgttga cgcggtaccg gaaccgattc
     5101 gatagcaggc ggcggacgga ggcttcggcc tccgtcgccg ttttcaggaa gcgcgcgccc
     5161 cgaagggggc gctggaggag cgggacgcca tgagcgacac gggacgcttc catcggacgt
     5221 tgctgccgtc cggcctcacc gtgatcggcg aggagatgga ctccgtccgc tcgctcgcca
     5281 tcggcgtctg gatgaaggtg ggcgcccgcc acgaggacgc cgcgaccagc ggcatgtccc
     5341 acttcctgga gcacatggtc ttcaaggggt cgcgccgccg cgacgcctac cagatcgcgc
     5401 tctcgctgga gtcggtgggg gggcacctgg acgcgttcac ggggcgggag tcgacctgct
     5461 tctacgcgcg cgcgctcgag gaccacctgg acctcgcggt ggacgtgctc gccgatctct
     5521 ccctctcccc gcggctcgac cccgccgacg tggccaagga gaagcaggtc gtggccgagg
     5581 agatccacaa ctacgacgac acgcccgacg actgcgtgca cgacctgttc gcggacgtcg
     5641 tctggaacgg gcatcccctg ggaaaccgca tcctcggaag cgaggagtcg gtgaccgcgt
     5701 tcacccccga ggaggtggcc gcctatcacg gccgccacta cacggcgggg aacatgatcg
     5761 tggcgatcgc cggacgcttc gactggccgc gcgtcgtgga cctggtggag cgccatttcg
     5821 ccccggcccc ggcgggcgcg ccgccggcgc ctcctcgcgc cgccaacggc gcggggcgcg
     5881 acgtcgtcca tcacgtgcgc gacctggcgc agcagtacct gtgcatcggc gccccggggc
     5941 ttcgccagga ggaccccgac cgctacgcgc tggtgcttct ttcgacggtg ctgggcggcg
     6001 gtatgtcgtc gcggcttttc cagcgcgtgc gggagcagga ggggctcgcc tactccgtct
     6061 acacctattc cgattcctac gaggacgccg gcatcttctg cgccgcgatg agcgtccacc
     6121 cctcgcaggg gcggaaagcg gtacgcctca cgctcgagga gttcgaccgg atcatcgagg
     6181 ccgggatcac tcccgaggag ctctcgtcgg ccaaggagca gttgaagggg agcctgctcc
     6241 tcggcctgga gagcacctcg aaccggatgc accggatcgc gcgctccatc ctctacagcg
     6301 ggcgcttcct cccggtcgac gagctggtgc gcaccatcga ccggatcacc gccgaggagg
     6361 tccgcgagat ggccgtgcgc gtgctggatc gcgaccggct ctccctggtc gccctgggcg
     6421 ccgatcagaa cggcgcgttc tccgaagcgg acctgaacca ccgggaggtc gcatgaccgc
     6481 ggacgcgcgg gagtcccaag ccgtggagcg acgtgagacg agagcgccgg aagcggcgga
     6541 gatcggggtg cgggtcacgc tcctcccgca cgccgcgggg gcgcccgcct acgagaccga
     6601 gcacgccgcg ggcatggatc tccgcgccgc gatcgagggg gagctggtgc tcgcccccgg
     6661 ccacgtggcg ccggtgccga ccggcatccg gatcgagatc ccacccggct acgagggaca
     6721 ggtccgcggg cggagcggcc tggcgctccg gcacgcggtg ggggtgccca atgctcccgg
     6781 cacgattgac gccgactacc gcggcgaggt gacggtcctt ctcgtgaact ggggcaccga
     6841 gccgtacacg gtgcgccggg gagaccggat cgcgcagctg gttatcgcgg cggtggcgcg
     6901 ggcccgcctg gagatcgcgg agtcgctcac ggagacggcg cggggcgcgg gcggcttcgg
     6961 ccacaccggc cgttgaggcg cgcccagccc gggtgggaac gggccgcggg acgtacgggt
     7021 tcctaacggt acgatgcccg acccttccgg ccgctccctt ccgatcgcga tcctgacggc
     7081 cctcgcctgc gccggcgttc t
//
LOCUS       DASSLZ010000084         9256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_150185, whole genome shotgun sequence.
ACCESSION   DASSLZ010000084 DASSLZ010000000
VERSION     DASSLZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150185"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFM00_03035"
     CDS             complement(<1..459)
                     /locus_tag="VFM00_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7497250.1"
                     /translation="MTTPLQQTGDYARGMRSSLVGMGVNVLLATGKLVAGILGTSYAL
                     VADAIESLGDVVGSIVVWRGLQIASRPPDHNHPYGHGKAEPIAAALVSVMLVAAGTGI
                     AFQAAREIASPHRAPQPFTLAVVLGVVAIKEGMFRFVHRVGSETGSHAVRT"
     gene            complement(456..932)
                     /locus_tag="VFM00_03040"
     CDS             complement(456..932)
                     /locus_tag="VFM00_03040"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7497251.1"
                     /translation="MNPKRDHAGIFVPPPFLYAIPFAAGMLLHRTVGGDSFPPSIAPA
                     ARIAGLALLAAGILLALASEILFARAGTSAIPIRPTQAIVTGGPYRFTRNPMYLSLAI
                     DYVGLTLVVGYAWPFAGLLVAILAVDRYAIPREERYLEGKFGDSYRQYRQSVRRWL"
     gene            complement(946..2100)
                     /locus_tag="VFM00_03045"
     CDS             complement(946..2100)
                     /locus_tag="VFM00_03045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7497252.1"
                     /translation="MASQPTAGVSSPRNQGSPAYRRPRLAVRLIALGLSVLVALLLLE
                     GAVRLRQWIRYGTSKAEVVELVTDPATGLRIARPNLDNGRIRTDSHGFRNPEIAMPKP
                     LDRVRLAFLGGSTTFCAEVSSNDVTWPALVVRKLAARYPDVAFDFVNGGQPGYGTESS
                     LKSLRLRIAPLQPDFVLYYEATNDLSTDTRELARRRGLFHGDADKPSPLARFSSAYAL
                     VEKTLRYRTRAAGSASGPRLVYDADSLARGFQARLVELIRTAHQTAPLCALATFSHKV
                     RRDQPPDVQRKACESSLYYAPYMSVPGLLDGWDAYNRAIAAAARETGALLIGGEDAIP
                     GDDVHFADSVHFRDAGSVLMADRIANALIASPEFNRLVAAHRAGGKAPSS"
     gene            2131..3963
                     /locus_tag="VFM00_03050"
     CDS             2131..3963
                     /locus_tag="VFM00_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HET7497253.1"
                     /translation="MTRILGISAFYHDSAAALIVDGEIVAAAQEERFTRKKHDPEFPV
                     RAVEYCLREAGITADQLDYVGFYDKPILKFDRLLETYLAYAPAGFKSFLQAMPVWLRK
                     KLYLKRELNRGLGGGYTKRYVFTEHHESHAASAFFPSPFEEAAILTLDGVGEWATASY
                     GYGRGNKIHLTHELRFPHSLGLLYSAFTYFCGFRVNSGEYKLMGLAPYGEPKYADFIR
                     AKMIDLKEDGSFRMDMSYFNYCQGLTMTSKKFDQAFGGPPRKPESPLTQREMDLAASI
                     QRVTEEIMMRCARHLHRETGMKNLCLAGGVALNCVGNGKILRDGPFENVWIQPAAGDA
                     GGALGVALFIEHQLLDRPRRVERLDRQRGSLLGPRFGTEEVRAFLDKVGAKYRRFDTD
                     DELCEYVAGQIASEKVVGWCQGRMEFGPRALGSRSILGDARSEQMQKVMNVKIKFRES
                     FRPFAPSVLQERVAEYFEVRPEEPSPYMLITSPVRETIRLKTNGDGLTGIDKLKVKRS
                     SVPAITHVDYSARMQTVDKDRHGRYYKLLKSFEAKTGCPVIINTSFNVRGEPIVCRPE
                     EAYRCFLATNMDVLVLEDCVLHKEDQPESAKPKVEEYLAQFKLD"
     gene            3966..4382
                     /locus_tag="VFM00_03055"
     CDS             3966..4382
                     /locus_tag="VFM00_03055"
                     /inference="COORDINATES: protein motif:HMM:NF039992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HET7497254.1"
                     /translation="MSVIEINKNPSRRDLLVFGLMLVAFTGLLGAMFLWRTHSMAVAR
                     AVWYGGGALSAIYFVLPPLRKPIFLGWTYLTFPIGFVISHVILGFVYFVVFTLVGLAM
                     RLVGYDPMQRKLDPSAKSYWVAHDPGTSVERYFRQS"
     gene            4438..4602
                     /locus_tag="VFM00_03060"
     CDS             4438..4602
                     /locus_tag="VFM00_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HET7497255.1"
                     /translation="MASAKSGNLFGEVWAFLSHNKKWWLLPLIVVLVMVGALIVLSTT
                     AVAPFIYTLF"
     gene            complement(4643..5134)
                     /locus_tag="VFM00_03065"
     CDS             complement(4643..5134)
                     /locus_tag="VFM00_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7497256.1"
                     /translation="MTQQTTTDRFQKEIHTFLEETFDKVHGIYLDKGTSFLETLDAVT
                     AAEASRRAAAGSGSVAAHVKHVSYYLRVLQQALRGEALGQLNWREIWEQDRPVSPEAW
                     KGITDELRGELANLRKLLNEPATWEREDAVGESMAVLVHTAYHLGAVRQALVVIRGRN
                     DAA"
     gene            complement(5188..6789)
                     /locus_tag="VFM00_03070"
     CDS             complement(5188..6789)
                     /locus_tag="VFM00_03070"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HET7497257.1"
                     /translation="MALRAVRVLALGIAAAALLVLVAITLGIPTALGVPLSRFREPTW
                     ALWAALALFVTAHERPRESWRRAVGRAVSILGSGPFFGRMAATLFALYFLVSVRIHLS
                     FHTFSHDFSMFDESLWWSHHGRFLYAPVLGRSYLTEHFSPILALLVPLHALFSSPWLL
                     VVANAALLGTAVFPLRAMLDAIGLPPAARNVACLVYLTSPITGSALDYGFHVESFLPL
                     LVFSLYAAHLQGPAWAYAILLVAALAIKEDVGLYLVGFGAFLFVAEKRRARGALTAAA
                     GLAWVLLVVWVVSPRLAGASGEYRFLSRWSQWGSGIGGVLGGMAAHPGAVLLALTAPT
                     YLLFFWRLVFTPFFTRWGWLLFVIPWALGATSGSRPQATLGLYYGLPLLAFAGLAAAR
                     GVMAVHGTRSVSESRGPVGVQVAALAPALAAIAIALNVSHFTIQPIPPGRDAVLTEIR
                     RIPPGATVQAMPTLYPDLRYEQPKAVLMPGDSLTAQYVLLRTDTTAWPFSTVQVNALT
                     ARAIASGTYRTHFDRGGFRVLMRRS"
     gene            complement(6791..7705)
                     /locus_tag="VFM00_03075"
     CDS             complement(6791..7705)
                     /locus_tag="VFM00_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497258.1"
                     /translation="MTRSNPTISGLAATVLAAGILLSTPACQRATQNASKPDSVAVAQ
                     ADSLAVRNLVLTFGNRLKQVSLLAPDSARARAMEAQYGGLVAPALLKQWAADPRHAAG
                     RITSSPWPDHIEIGNVARKSTRVYLVDGEIVERTSADSAGASGRIPVRIAVWRVGRAW
                     MIQNYEQGQPGAGTTDTGEDDSPERVTAVVRAYYDAINAGRFQNAYRMWEADGAASGK
                     SMIEFLNGYAQTKSVEATIGTPGPMGAAAGSRYVDVPVHLVATTVTGAREAFSGTITL
                     RRSVVDGATPKQRTWQIYSAKLKKEPAA"
     gene            7786..8328
                     /locus_tag="VFM00_03080"
     CDS             7786..8328
                     /locus_tag="VFM00_03080"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET7497259.1"
                     /translation="MTSYVALLRAVNLAGRNRVGMADLRAVAEGLGYAEPRTLLQSGN
                     LLLRGPARPAVALERDLREAAKKKLGMEIDFFVRSAAEWQALVAKNPFPREARSDPGH
                     LIAIALSGAPGGTELEALEAAITGRERVRLAGRTAYAVYPDGVGRSKLTMALIEKKLG
                     VRGTGRNWNTVLKLAALLNE"
     gene            complement(8480..8947)
                     /locus_tag="VFM00_03085"
     CDS             complement(8480..8947)
                     /locus_tag="VFM00_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7497260.1"
                     /translation="MEDPDDRPDPTTPAERIEVEDAITTLFIATDEKDWAGVRGCLAD
                     EVLFDMSSLSGVAAASVSADSIVGAWKQGLAPMQAIHHQVGNFRVCVHGEEAVATCYG
                     IAYHYRPNFSGKNTRVFVGTYDFHLRCAWGKWRIDRFRFQVKFVDGNLELEKM"
     gene            complement(8949..>9256)
                     /locus_tag="VFM00_03090"
     CDS             complement(8949..>9256)
                     /locus_tag="VFM00_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497261.1"
                     /translation="TVMNDPGNFTLHIGSVENFSHRMEYRWENPAAKATVRQASSVTG
                     GDGTLEVRDAAGRRVHSKSLREQGSFATTEGKPGTWRIGVILRRTTGSVTFEVRRSD"
BASE COUNT         1549 a         3155 c         3175 g         1377 t
ORIGIN      
        1 ggtccggacc gcgtggcttc cggtctcgct cccgacgcgg tgcacgaagc ggaacatccc
       61 ctccttgatg gccacgaccc ccaggacgac cgccagcgtg aagggctgcg gcgcccgatg
      121 cggcgaggcg atctcgcggg cggcctggaa cgcgatcccc gtcccggccg ccaccagcat
      181 caccgagacc agggccgccg cgatcggctc cgccttgccg tggccgtagg gatggttgtg
      241 atccggggga cggctcgcga tctggagccc gcgccacacg acgatcgagc cgaccacgtc
      301 gcccagcgac tcgatggcgt cggcgaccag cgcgtaggag gtgcccagga tccccgccac
      361 cagctttccc gtcgcgagca ggacgttcac gcccatcccg accaggctgg agcgcatccc
      421 gcgcgcgtag tcgccggtct gctggagcgg ggtcgtcata gccagcgccg gacggactgg
      481 cggtactgcc ggtacgaatc gccgaacttc ccttccaggt agcgctcctc gcgcgggatg
      541 gcgtatcggt ccaccgcgag gatcgcgacc aggagtcccg cgaagggcca tgcgtacccc
      601 acgaccaggg tgagcccgac gtagtcgatg gcgagggaca ggtacatcgg gttccgggtg
      661 aagcggtacg gccccccggt cacgatcgct tgcgtcggcc ggatcggaat cgcggaggtg
      721 cccgcgcgcg caaagaggat ctccgaggcc agggcgagga ggattcccgc cgcgagcagg
      781 gcgaggccgg cgatgcgcgc cgcgggcgcg atggacggcg gaaaggaatc gcccccgacg
      841 gtccggtgga gcagcatccc cgccgcgaag ggaatcgcgt agaggaaggg cggcggcacg
      901 aagatccccg cgtgatcgcg cttcggattc atcgccgcgc gcgcctcacg acgacggcgc
      961 cttcccgccc gcgcgatggg cggccacgag gcggttgaat tccggcgagg cgatcagcgc
     1021 gttcgcgatc cgatcggcca tgaggaccga gcccgcgtcc cggaaatgga cggagtcggc
     1081 gaagtggacg tcgtcccccg ggatcgcgtc ctccccgccg atcaggagcg cgccggtctc
     1141 gcgcgcggcg gccgcgatgg cccggttgta ggcgtcccac ccgtccagaa gccccggcac
     1201 cgacatgtac ggagcgtagt acaggctgga ttcgcacgcc ttgcgctgca cgtccggcgg
     1261 ctgatcgcgc cggaccttgt gcgagaaggt cgcgagggcg cacaggggcg cggtctgatg
     1321 tgccgtccgg atcagctcga ccagccgcgc ctggaatccc cgcgcgagcg agtccgcgtc
     1381 gtagacgagg cgcgggccgg acgcgctgcc ggccgcgcgg gtgcggtagc gcagcgtctt
     1441 ctccaccagc gcgtacgcgg acgagaagcg cgcgagcggg ctcggcttgt cggcgtcgcc
     1501 atggaacagg ccgcgccgcc gcgccagctc gcgcgtgtcg gtgctcaggt cgttcgtcgc
     1561 ctcgtaatag aggacgaaat cgggctggag cggagcgatc cgcagcctca ggctcttgag
     1621 cgaggattcg gtgccgtatc ccggctggcc cccgttcacg aagtcgaacg cgacgtcggg
     1681 gtagcgtgcg gcgagcttcc gcacgaccag ggcgggccag gtcacgtcgt tgctcgagac
     1741 ctcggcgcag aaggtggtgg acccacccag gaaggccagc cggacccggt cgagcggctt
     1801 gggcatggcg atctcggggt ttcggaagcc gtgcgaatcg gtccggatgc gcccgttgtc
     1861 caggttcggc ctggcgatgc ggagcccggt ggcggggtcg gtgaccagct ccaccacctc
     1921 ggccttgctc gtgccgtagc ggatccactg ccggagccgg acggcccctt ccagcagaag
     1981 cagggcgacg agaacggaga ggcccagcgc gatgagacga acggcgaggc gggggcggcg
     2041 gtaagccggg gatccctgat tccgcggtga cgagaccccg gcggtcggtt gagacgccat
     2101 cgcggcgcat ggtatcattt ccgccttctc atgactcgaa ttctaggcat ctcggccttc
     2161 tatcacgact ccgccgccgc cctcatcgtg gacggcgaga tcgtggcggc cgcccaggaa
     2221 gagcgcttca cgcgcaagaa gcacgacccg gagtttccgg ttcgcgcggt cgagtactgc
     2281 ctgcgcgagg cgggcatcac ggccgaccag ctcgactacg tcggcttcta cgacaagccg
     2341 atcctcaagt tcgaccgcct tctggaaacc tacctggcct acgcgccggc cggcttcaag
     2401 tccttcctcc aggcgatgcc ggtctggctg cggaagaagc tctatctgaa gcgcgagctg
     2461 aaccgcgggc tgggcggcgg ctacacgaag cgctacgtct tcaccgagca ccacgagtcg
     2521 cacgcggcca gcgccttctt cccctccccg ttcgaggagg cggccatcct cacgctggac
     2581 ggcgtgggcg agtgggcgac ggcgagctac ggctacggcc gcggaaacaa gatccacctg
     2641 acgcacgagc tccgcttccc gcactcgctg ggactcctct actccgcctt cacctacttc
     2701 tgcggcttcc gcgtcaattc cggcgagtac aagctgatgg gcctggctcc ctacggcgag
     2761 ccgaaatacg ccgacttcat ccgcgcgaag atgatcgacc tgaaggagga tggctccttc
     2821 cggatggaca tgtcgtactt caactactgc cagggcctga cgatgacgtc gaagaaattc
     2881 gaccaggcct tcggcggccc gccccggaaa cccgagagcc cgctcacgca gcgcgagatg
     2941 gacctggccg cctcgatcca gcgcgtcacc gaggagatca tgatgcgctg cgcgcgccac
     3001 ctgcatcgcg agacggggat gaagaacctc tgcctcgcgg gcggcgtcgc gctgaactgc
     3061 gtcggcaacg gaaagatcct gcgcgacgga cccttcgaga acgtctggat ccagcccgcc
     3121 gccggcgacg ccgggggcgc gctcggcgtg gcgctcttca tcgagcacca gctcctcgac
     3181 cggccccggc gggtggagcg gctggaccgc cagcgcggtt cgctgctcgg tccgcgcttc
     3241 ggcaccgagg aggtgcgcgc gttcctcgac aaggtggggg ctaagtatcg ccgcttcgat
     3301 accgacgacg agctgtgcga gtacgtggcc ggccagatcg cttcggagaa ggtggtcggc
     3361 tggtgccagg ggcggatgga gttcggaccg cgcgcgctgg gcagccggag catcctgggc
     3421 gacgcccgca gcgagcagat gcagaaggtc atgaacgtga agatcaagtt ccgcgagtcg
     3481 ttccgcccct tcgcgccctc ggtgctccag gagcgggtgg cggaatactt cgaggtgcgc
     3541 ccggaggagc cgagtcccta catgctgatc acctcgccgg tccgcgagac gatccgcctc
     3601 aagaccaacg gcgacgggct gaccggaatc gacaagctca aggtgaagcg ctcgagcgtt
     3661 cccgcgatca cccacgtgga ctactcggcg cgcatgcaga ccgtggacaa ggaccgccac
     3721 ggccgatact acaagctgct caagagcttc gaggccaaga ccggctgccc cgtcatcatc
     3781 aacacgtcct tcaacgtgcg cggcgagccg atcgtctgcc gtccggaaga ggcgtaccgc
     3841 tgcttcctcg cgaccaacat ggacgtgctg gtgctggagg attgcgtgct ccacaaggag
     3901 gaccagcccg agagcgccaa gccgaaggtc gaggaatacc tggcgcagtt caagctggac
     3961 tgaccatgag cgtgatcgag atcaacaaga accccagccg cagggacctc ctggtcttcg
     4021 gcctgatgct ggtcgccttc acgggactgc tcggcgcgat gttcctgtgg cgcacccact
     4081 cgatggccgt cgcgcgcgcc gtctggtacg gcggcggcgc gctcagcgcg atctacttcg
     4141 tgctgccgcc gctccgcaag ccgatctttc tgggctggac ctacctcacg ttccccatcg
     4201 ggttcgtgat ctcgcacgtc attctcggct tcgtctactt cgtggtgttc accctggtgg
     4261 gcctggcgat gcggctggtc gggtacgatc cgatgcagcg gaagctggat ccctccgcga
     4321 aatcctactg ggtcgcgcac gatcccggca ccagcgtcga gcgctacttc cgccaatcct
     4381 gagacggaga tcatgagcga accaacgaac ggagaagacg ggcgcgagtt cgaccgcatg
     4441 gcgtcggcga agtcggggaa tctcttcggc gaggtctggg cgttcctgag ccacaacaag
     4501 aagtggtggc tcctgccgct gatcgtcgtc ctggtgatgg tgggcgcgct gatcgtgctg
     4561 agcaccaccg ccgtggcgcc gttcatctac acactgttct agaaccgggc gccccgcgct
     4621 tcgtacgggg cgtcgcggag cgctaggcgg cgtcgtttcg gccgcggatc acgaccagcg
     4681 cctggcgcac cgcgcccagg tgatacgcgg tgtgcaccaa caccgccatc gattccccga
     4741 ccgcgtcctc ccgctcccac gtggcgggct cgttcaggag cttcctcaag tttgcgagct
     4801 caccgcgcag ctcgtcggtg atccccttcc acgcctcggg ggacaccggc cggtcctgct
     4861 cccagatctc ccgccagttc agctggccca aggcctcccc gcgcagcgcc tgctgcagca
     4921 cgcgaaggta gtaggagacg tgcttcacgt gtgcggcaac gctgcccgac ccggccgccg
     4981 cgcgccgtga ggcctccgcg gcggtaaccg cgtccagggt ttcgaggaac gaggtcccct
     5041 tgtcgaggta gatgccgtgg accttgtcga aggtctcctc gaggaaggtg tggatctcct
     5101 tctggaatcg atcggtggtc gtctgctggg tcattgcgaa tctcctcgtt gcggagcgaa
     5161 cgtggacagg ggtgcgagcg tagcaagtca ggagcggcgc atcagcaccc gaaagccgcc
     5221 gcgatcgaag tgcgtccggt atgtccccga cgcgatggcc cgggccgtca gggcgttcac
     5281 ttgcacggtc gagaacggcc aggcggtcgt gtcggtccgc agcaggacgt actgcgccgt
     5341 gagcgaatcc ccgggcatca gcacggcctt gggctgttcg taccgaagat cgggatacag
     5401 cgtgggcatc gcctgcactg tggcgccagg cggaatgcgc cggatctcgg tcaggacggc
     5461 gtcgcgtccc ggcgggatcg gctggatggt gaagtgggag acattgagcg cgatcgcgat
     5521 cgcggccagg gccggggcga gggcggcgac ttggactccc acgggtcctc tcgactccga
     5581 gacgctccgg gttccgtgga ccgccatgac tccacgtgcc gccgcgagcc ccgcgaacgc
     5641 gagcagcggg agcccgtaat agagtccgag ggtggcctgc ggacgcgaac ccgatgtggc
     5701 acccagcgcc cacgggatca cgaacaggag ccacccccat cgcgtgaaga aaggggtgaa
     5761 gaccagccgc cagaagaaaa gaaggtaggt cggcgcggtc agggccagga gaacggcgcc
     5821 cggatgcgcc gccatgccgc ccaggacccc gccgattccg ctcccccact gtgaccagcg
     5881 gctcaggaac cgatactcgc ccgaggcacc ggcgagtcgc ggactcacca cccacaccac
     5941 cagcagcacc catgcgagtc ccgccgcggc ggtcagcgcg ccgcgcgcgc gccgcttctc
     6001 cgccacgaag aggaaggcgc cgaaccccac caggtagagt cccacgtctt ccttgatcgc
     6061 gagcgcggcc acgagaagga tcgcgtacgc ccacgccggt ccctggagat gcgccgcgta
     6121 gagcgagaag acgagaagcg gcagaaacga ctccacgtgg aagccgtagt cgagcgccga
     6181 accggtgatc gggctcgtga gatagaccag acacgccacg ttccgcgcgg ccggcggcag
     6241 tccgatggcg tcgagcatcg ctcgcagcgg gaacacggcg gtcccgagga gcgccgcgtt
     6301 cgcgaccacg agcagccacg ggctggagaa gagcgcgtgc agcggcacca ggagcgcgag
     6361 gatcggcgag aagtgctcgg tcaggtagct ccggcccagg acgggcgcgt agagaaaccg
     6421 tccgtggtgc gaccaccaga ggctctcgtc gaacatcgag aagtcgtgcg agaaggtgtg
     6481 gaacgagaga tggattcgga ccgagaccag gaagtagagc gcaaagaggg tggccgccat
     6541 gcggccgaag aaggggcccg atccgaggat ggaaaccgcg cggccaacgg cccggcgcca
     6601 cgactcgcgg ggccgctcgt gagccgtgac gaacagggcc agcgcggccc aaagcgccca
     6661 ggtcggctcg cgaaagcgcg agagcggcac gcccagcgcc gtggggatgc ccagcgtgat
     6721 cgcgacgagt acgagaagtg cggccgccgc gatccccagc gcgagaacgc ggaccgcgcg
     6781 cagcgccacg ctaggcggcc ggctccttct tcagcttggc cgagtagatc tgccaggtgc
     6841 gctgcttggg cgtggcgccg tccacgacgc ttcgccggag cgtgatggtt ccggagaacg
     6901 cctcgcgggc gcccgtcacg gtcgtggcga ccaggtggac cggcacatcc acgtaacgcg
     6961 accccgccgc ggcgcccatc ggccccggcg ttccaatcgt ggcttcgacc gacttggtct
     7021 gggcgtagcc gttcaggaac tcgatcatgc tctttccgct cgcggcgccg tcagcttccc
     7081 acatgcggta ggcgttctgg aaccgccccg cgttgatggc gtcgtaatag gcccggacga
     7141 ccgcggtgac gcgctcgggc gaatcgtcct cgccggtgtc cgtcgtcccc gcgccaggct
     7201 ggccttgttc atagttctgg atcatccacg ctcggcccac cctccagacg gcgatccgca
     7261 ccgggatccg tcccgaggcg cccgccgaat ccgcgctcgt gcgctcgacg atctcgccgt
     7321 ccaccaggta gacccgcgtg gactttcgcg cgacgttccc gatctcgatg tggtcgggcc
     7381 aggggctgga cgtgatcctt cccgcggcgt ggcgcggatc ggcggcccat tgtttgagca
     7441 gggccggagc gaccagcccg ccgtactgcg cctccatcgc gcgggcgcgc gcgctgtcgg
     7501 gcgcgagcag cgagacctgc ttgagccgat ttccgaacgt gagcacgaga tttcgcacgg
     7561 cgagcgaatc cgcctgcgcc acggcgactg agtcgggctt ggatgcgttc tgcgtggcgc
     7621 gctggcaggc gggagtggaa aggaggatgc ccgcggcgag aacggtcgct gcgagcccgg
     7681 agatcgtcgg attggaacgc gtcatgggaa gcgccctcgg cccttgtcga aaatcaggtg
     7741 ggagagtata gcccgggcgc ccggcgcgcc agtatcctgt cgggcatgac ctcctacgtg
     7801 gccctgctcc gcgccgtcaa cctggcgggg cgcaacaggg tcgggatggc cgacctgcgc
     7861 gcggtagccg aagggctggg ctacgccgag ccgcgcacgc tgctccagag cggcaatctc
     7921 ctgctccgcg gacctgcccg gcccgccgtg gcgctggagc gcgacctgcg cgaggccgcg
     7981 aagaagaagc tcgggatgga gatcgatttc ttcgtgcgct ccgccgccga atggcaggcg
     8041 ctcgtggcga agaacccctt cccgcgcgaa gccaggagcg atcccggcca cctcatcgcc
     8101 atcgcgctct ccggcgcgcc cggaggcacg gagctagagg cgctggaagc ggcgatcacc
     8161 ggacgcgagc gcgtccgcct ggccggccgc accgcctacg ccgtctaccc cgacggcgtc
     8221 ggacgctcga agctcacgat ggcgctcatc gagaagaagc tgggcgttcg agggaccggc
     8281 aggaactgga acacggtact gaagctcgcc gcgttgttga acgaatgaag gatcgccaag
     8341 gtgaacgaca agtgtcgcga cttacacact cgccagcggc ggtgaatccg acgacaactg
     8401 tcgcgaaatt cacacgcgcc agagcgggtg aacccgacga caggcgccgc agcgacgcgc
     8461 cagcgccgcc cgcctcccgc tacatcttct ccagctcgag attcccgtcc acgaacttga
     8521 cctgaaaccg gaagcgatcg atccgccact tcccccacgc gcacctcaga tggaaatcat
     8581 acgtgcccac gaacacgcgc gtattcttcc cggagaagtt cgggcgatag tggtaggcga
     8641 tgccgtagca cgtggccacc gcctcctcgc cgtgaacgca cacgcggaag ttccccacct
     8701 gatgatggat cgcctgcatg ggcgcgagcc cctgcttcca cgccccgacg atcgagtcgg
     8761 cggacaccga agcggcggcc acgcccgaca ggctcgacat gtcgaagagc acctcgtccg
     8821 caaggcatcc ccgtacgccg gcccagtcct tctcgtcggt ggcgatgaac aaggtcgtga
     8881 tggcgtcctc gacctcgatg cgctcggccg gcgtggtcgg gtcgggcctg tcgtccgggt
     8941 cttccacctt agtcgctcct ccgaacctcg aaggtcaccg agccggtcgt gcgccgcagg
     9001 atcacgccga tcctccacgt cccgggcttt ccctcggtcg tggcgaacga gccctgctcg
     9061 cgcaggctct tcgagtgcac ccgccggccg gcggcgtcgc gcacctccag agtgccgtcg
     9121 ccgcccgtga cgctggaggc ctggcgcacc gtggccttcg cggcggggtt ctcccaccgg
     9181 tattccatcc ggtgcgagaa gttctccacc gagccgatgt gcagggtgaa gttgccgggg
     9241 tcgttcatga ccgtca
//
LOCUS       DASSLZ010000085         7706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_791728, whole genome shotgun sequence.
ACCESSION   DASSLZ010000085 DASSLZ010000000
VERSION     DASSLZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791728"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFM00_03095"
     CDS             <1..219
                     /locus_tag="VFM00_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497262.1"
                     /translation="RRGAAAAIVTEKDEYRWSFPPDPPIPVLALRTDLVPLDPVDRLP
                     GIAPGPVRTNCGGEPVAAPSGAAAGAAT"
     gene            232..1860
                     /gene="nadB"
                     /locus_tag="VFM00_03100"
     CDS             232..1860
                     /gene="nadB"
                     /locus_tag="VFM00_03100"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HET7497263.1"
                     /translation="MPRRHNDLETEVLVIGSGIAGLSTALHAAQTTRVLVVTKVHAEE
                     SNTNYAQGGVAAVLGEDDKVALHERDTLIAGDGLCDPEAVRVLVEEGPGEVLRLMGLG
                     VRFSHDPEGRLALGREGGHSRRRIVHAKDRTGHAIERVLLKLARAHPRISILENQIAV
                     DLILDSKMRGRRCAPEKDRIWGAYVLDRESGAIQAIGARATVLATGGSGKVYLYTTNP
                     DIATGDGLAAGFRAGAAAADLEFMQFHPTCLFHPEAKSFLISEAVRGEGAHLLTLDGK
                     RFMQRYHRLKELAPRDVVARAIDFEMKKRGDKCVLLDMRPLGKRLLTTRFPHITSRLI
                     EYGFNPFREPVPVVPAAHYMCGGILTDLEGRATIPGLYACGEVACTGVHGANRLASNS
                     LLEAIVFSRRAGMALLRQVTGERPERIPAVPEWRYNGAVEPKEQVIFDHNWDAIRRVM
                     WDYMGIVRSDERLARAASMIAVLRDQTEGDYQRYRLDADLVELRNVGLVADLIIACAR
                     RRKESRGLHYNVDHLRRDDRRFRRDTVLTRQDVR"
     gene            1862..2272
                     /locus_tag="VFM00_03105"
     CDS             1862..2272
                     /locus_tag="VFM00_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Minf_1886 family protein"
                     /protein_id="HET7497264.1"
                     /translation="MSRDIETRMLELAQQYGRYKANAYRFTFDAVRYTADRNYQVTKE
                     VRHVTGIEVLEGIRQLALDQFGFMAKTVFLEWGIQRTEDFGEIVFQLVREGILSKTEK
                     DTPGDFARGYDFDEVFVRNYDWLDRLGEPRTHRA"
     gene            2280..4316
                     /locus_tag="VFM00_03110"
     CDS             2280..4316
                     /locus_tag="VFM00_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7497265.1"
                     /translation="MGRVKADRSPSLRLLRYLEPYRGHLALTAALMVVFALLSGISIG
                     MISPFVKILFRPVPVAGGPSAGTAATSGTPAPTPLGGIPGLDAAGVAGEATTATGPAP
                     QIGVKDAQGKPRPFAAITERGNQAKQKLRTWFEHFFLIGDPIRSLTRICLALLVVFLL
                     KNAVDYLQSVLTVWVEQAVIRDMRNQVYAHLQTLSLSFFHSRRTGALLSRFTNDIALV
                     RGALAAGFSNLIKSSLLLAVCLFWIFWTSWRLALVSLVVVPFSLVLIVWLGRKLRRRS
                     SITQERMGDLNAILQETLTGIRVVKAFAMEDFEQRKFERASQGYFRAFVKQRRLGALA
                     GPMSEYLGVVAATAVLWYGGREILLTRAIEPQQFFIFLFAMLQLMSPLKSLSNVNATL
                     QEGLAAAVRIFRILDTEATVVSRPNSRRIAGIHDGIEFEGVSFRYGQGPEVLRDVSFR
                     IEAGEVVALVGPSGAGKSTLADLVARFYDPTSGRILIDGVDLREYELSSWRSLLGVVT
                     QEPILFHDSVFHNIAYGVSDAGDEAVRRAARAAHADRFIDEMPEGYQSPIGERGVRLS
                     GGERQRIAIARAIFKDPRLLLLDEATARLDSQSELLVQEALEALFNGRTVLVIAHRLS
                     TIQRADRIVVVDQGRIVQMGTHAELVQTPGLYQKLHRLQFRLAEAGFPARPRAG"
     gene            4320..5399
                     /locus_tag="VFM00_03115"
     CDS             4320..5399
                     /locus_tag="VFM00_03115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7497266.1"
                     /translation="MARGAALVVRFSSLGDVLLAAHLPSFLRDADTGRRVLFATKERY
                     APILRGHPDVARFFLLEDGSSDPAAPAPFGLLGGLGLLASGLRREGIDEVYDLHQNLR
                     SSRLVSSLGQVRRVAPSKHGLRRRLMVHAKWLHPEPLPPLLGTYREIAGLNPAAPVRP
                     WLREALGDSERARARARLGEGGEERIALLGVGARWETKRWPLERFVELGERLHAETGL
                     SPRYAVLPKSAEASALRARLPEARYGAILEGSFRECAAAASFARVIVSNDSATLHLGA
                     ALGVPAVGIFGSTVPAFGFAPSGPRDAVVETALSCRPCSVHGRRRCPLGHHRCMRDIA
                     PDLVLAAVQRVLAGAEAAPPAEARA"
     gene            5396..6316
                     /locus_tag="VFM00_03120"
     CDS             5396..6316
                     /locus_tag="VFM00_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET7497267.1"
                     /translation="MSDRILLEGNDPSIRVAVERRAGIALPWAAAGEEARATVWFCPG
                     VPPAAARELPALRWIQTGWAGVETWFGRPEWREGVLLTRTVGDFPQRIAEYVIGYLLA
                     DALGVPEALRQMEARAWKRWTPGSLAGRSLLVAGHGAIGRRVAEAARGLGMSSRGIRR
                     GPLSSEDLALGVEEPSALEALLPAADVVVNLLPLTTATDSFWNAARFERMKEGSTFVN
                     VSRGATVDDEALIAALARRRPARAILDVFRDEPLPAGHPLRSAPGVWITPHVAGIGTS
                     EPLAAEFAANWLRYRDGAPLRNRVDRARGY"
     gene            6446..7528
                     /gene="tdh"
                     /locus_tag="VFM00_03125"
     CDS             6446..7528
                     /gene="tdh"
                     /locus_tag="VFM00_03125"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HET7497268.1"
                     /translation="MAVTTTKEPIVKQGMMWAIHKPSAAPGLFPTTVPIPSIGPKDVR
                     IRVTAFAICGTDLHIVEWDPWAQSRIRPPLVPGHEFTGVVESVGDEVSLVKPGDRVTA
                     ETHIYCGICYACRRGLQHLCQKVQILGVDRQGAFADYVSIPEQNVWKLHPDIPEEIAA
                     IHDPLGNAVHTALAGPVAGLRFAVFGCGPIGCFSIGVLKAAGASWVAAIDKNPGRLEL
                     AKAMGADFTVDVNIASASAEVLRETDGRGVDGMLEMSGHPQAFKDGFDMLANGGRVSL
                     LGIPSKPLEIDVAKEIVFRGAVVQGINGRLIFDTWFRMEALLLTGKLNVRPVITHVLG
                     WSEFDRAVELQRSGKAGKIVLKRDHP"
     gene            7525..>7706
                     /locus_tag="VFM00_03130"
     CDS             7525..>7706
                     /locus_tag="VFM00_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HET7497269.1"
                     /translation="MSPAPETMKPANPLAFLDEALDGLRREGLYRHLRLLSGEQKPRA
                     RFDGRDVINLSSNNYLG"
BASE COUNT         1067 a         2765 c         2676 g         1198 t
ORIGIN      
        1 cgccgcggcg cggccgcggc gatcgtcacg gagaaggacg agtaccgctg gtcgtttcct
       61 cccgatccgc cgatcccggt gctggcgctc cggaccgatc tggtgcccct cgaccccgtg
      121 gaccggcttc ccggcatcgc cccggggccc gtccgcacga actgcggcgg tgaaccggtc
      181 gcggccccct ccggggcggc ggccggcgcc gcgacctgac ccgaggaatc cgtgccccga
      241 cgccacaacg atctcgaaac cgaagtcctc gtcatcggca gcggcatcgc cggcctctcg
      301 acggcgctgc acgccgcgca gacgacgcgc gtcctggtcg tcacgaaggt ccacgcggag
      361 gaatcgaaca ccaactatgc gcagggcggc gtcgccgccg tgctcggcga ggacgacaag
      421 gtcgcgctgc acgagcgcga cacgctgatc gcgggggacg ggctctgcga tcccgaggcg
      481 gtgcgcgtcc tcgttgagga gggccccggc gaggtgctcc ggctgatggg tctgggggtc
      541 cggttctcgc acgacccgga gggccggctc gcgctggggc gcgagggcgg gcactcgcgc
      601 cggcgcatcg tgcacgccaa ggaccggacc ggccacgcga tcgagcgggt tctcctgaag
      661 ctcgcgcgcg cccacccgcg gatctcgatc ctcgagaatc agatcgcggt ggacctcatc
      721 ctcgactcca agatgcgcgg ccgccggtgc gctcccgaga aggaccggat ctggggcgcc
      781 tatgtgctcg accgcgagag cggagcgatc caggcgatcg gcgcccgcgc gacggtgctc
      841 gccacgggcg gctcgggcaa ggtgtacctg tatacgacca atcccgacat cgcgaccggg
      901 gacggactgg ccgcgggctt ccgcgccggc gcggccgcgg ccgatctcga gttcatgcag
      961 ttccatccga cctgtctgtt ccaccccgag gccaagtcgt tcctgatctc cgaggcggtc
     1021 cgcggcgagg gcgcgcacct gctcacgctg gatgggaagc ggttcatgca gcgctaccac
     1081 cggctgaagg agctcgctcc tcgcgacgtg gtggcgcgcg cgatcgactt cgagatgaag
     1141 aagcgcgggg acaagtgcgt cctgctggac atgcgcccgc tcgggaagcg gctcctcacg
     1201 acgcgcttcc cgcacatcac gagccggctc atcgagtacg ggttcaatcc cttccgcgag
     1261 ccggtgccgg tggtgccggc ggcgcactac atgtgcggcg gcatcctgac cgacctcgag
     1321 ggacgcgcca cgatccccgg gctctacgcg tgcggggagg tggcctgcac cggggtgcac
     1381 ggcgcgaacc ggctggcctc caactccctc ctcgaagcga tcgtcttctc ccgccgggcg
     1441 ggcatggccc tccttcgtca ggtcaccggc gagcgccccg agcgcattcc ggcggtcccg
     1501 gaatggcgct ataacggcgc cgtggagccc aaggagcagg tgatcttcga ccacaattgg
     1561 gatgccatcc ggcgggtcat gtgggactat atgggcatcg tccggagcga tgaacgcctc
     1621 gcgcgggccg cttccatgat tgccgtcctg cgcgaccaga ccgagggcga ctaccagcgc
     1681 taccgtctcg acgcggacct ggtggagctt cggaacgtcg gcctcgtcgc cgacctgatc
     1741 atcgcctgtg cccggcgccg caaggagagc cgaggcctcc attacaacgt cgaccatctc
     1801 cggcgggacg accgccgctt ccgccgcgac acggtgctga cccgacagga cgtgcgctga
     1861 gatgagccgc gacatcgaaa cccggatgct cgagctggcg cagcagtacg ggcgctacaa
     1921 ggccaacgcc taccgcttca cgttcgacgc ggtgcgctac acggccgacc gcaattacca
     1981 ggtcacgaaa gaagtccggc acgtgaccgg catcgaagtc ctcgagggga tccggcagct
     2041 cgccctggac cagttcgggt tcatggccaa gacggtcttc ctcgaatggg gcatccagcg
     2101 gaccgaggac ttcggcgaga tcgtcttcca gctcgtgcgc gaggggatcc tgagcaagac
     2161 cgagaaggac accccgggcg acttcgcgag gggctacgac ttcgacgagg tcttcgtccg
     2221 gaactacgac tggctcgacc gcctgggcga gcctcggacc caccgggcgt agccgccccg
     2281 tgggccgcgt caaagccgac cgaagccctt cgctccggct cctccgctac ctcgagccct
     2341 accgggggca tctcgccctc accgccgcgc tcatggtggt cttcgccctc ctgagcggca
     2401 tctcgatcgg gatgatctcc cccttcgtga agatcctgtt ccggcccgta cccgtcgcgg
     2461 gcggaccctc ggccggaacc gccgcgacct ccggaacccc ggcccccacg ccgctcgggg
     2521 ggatccccgg gctggacgcg gccggggtcg ccggggaagc gacgacggcc acgggtccgg
     2581 cgccgcagat cggcgtgaag gacgcgcagg gcaagccgcg ccccttcgcc gcgatcaccg
     2641 agcgcggcaa ccaggcgaag cagaagctcc ggacctggtt cgagcacttc ttcctgatcg
     2701 gagacccgat ccgctcgctc acgcggatct gtctcgcgct cctcgtcgtc ttcctgttga
     2761 agaacgccgt cgactacctc cagagcgtgc tcacggtctg ggtggagcag gcggtgatcc
     2821 gcgacatgcg gaaccaggtc tacgcgcacc tccagaccct gtcgctctcc ttcttccact
     2881 cgcggcgcac gggcgcgctc ctctcgcgct tcacgaacga catcgcgctg gtgcggggcg
     2941 ccctggcggc ggggttcagc aacctgatca agtcctcgct ccttctcgcg gtctgcctct
     3001 tctggatctt ctggacctcc tggcggctcg cgctcgtgtc gctggtcgtc gtccccttct
     3061 cgctcgtgct gatcgtctgg ctggggcgaa agctccgccg gcggagcagc atcacgcagg
     3121 agcggatggg ggacctgaac gcgatcctcc aggagaccct gaccggcatc cgcgtcgtga
     3181 aggccttcgc gatggaggat ttcgagcagc gcaagttcga gcgcgcctcc cagggctact
     3241 tccgcgcgtt cgtgaagcag cgccgcctgg gcgcgctcgc cggaccgatg tcggaatacc
     3301 tcggcgtcgt cgccgccacc gcggtgctct ggtacggcgg ccgcgagatt ctcctcacgc
     3361 gcgccatcga gccgcagcag ttcttcatct tcctcttcgc catgctgcag ctgatgagcc
     3421 ccttgaagtc gctctcgaac gtgaacgcca cgctccagga ggggctggcc gccgcggtcc
     3481 gcatcttccg gatcctcgac acggaggcga ccgtggtctc gcgcccgaac tcgcggcgga
     3541 tcgccgggat ccacgacggg atcgagttcg agggggtctc cttccgctac gggcagggcc
     3601 ccgaggtgct gcgcgacgtc tccttccgga tcgaggccgg cgaggtggtc gcgctcgtcg
     3661 gaccgagcgg ggccgggaaa tccacgctgg ccgatctggt cgcgcgcttc tacgacccga
     3721 cgtccgggcg catcctcatc gacggcgtgg atctccgcga gtacgagctc tcttcctggc
     3781 gctcgctcct cggggtggtg acccaggaac caatcctctt ccacgactcg gttttccaca
     3841 acatcgccta cggcgtctcg gacgcgggcg acgaggccgt gcgccgcgcc gcgcgggcgg
     3901 cacacgccga ccgcttcatc gacgagatgc cggagggcta ccagagcccg atcggcgagc
     3961 gcggggtgcg cctctccggc ggcgagcggc agcggatcgc gatcgcccgg gccatcttca
     4021 aggacccgag gctcctcctg ctcgacgagg ccaccgccag actggactct cagtcggagc
     4081 tgcttgttca ggaggcgctc gaagccctct tcaacggccg cacggtgctc gtgatcgcgc
     4141 accgcctctc gacgatccag cgggcggacc gcatcgtggt ggtggaccag gggcggatcg
     4201 tgcagatggg cacgcacgcc gagttggtcc agacgccggg gctgtaccag aagctccacc
     4261 ggctccagtt ccgtctcgcg gaagccggat tcccggcacg tccgcgggcg ggctgaggga
     4321 tggcgcgcgg cgccgctctc gtcgtccgct tctcctcgct cggcgacgtc ctcctcgcgg
     4381 ctcatcttcc gtccttcttg cgcgatgccg atacgggccg gcgcgtcctc ttcgccacga
     4441 aggagcgcta tgcgccgatc ctgcgcggcc atcccgacgt ggcgcgcttc ttccttctgg
     4501 aggatggcag ctccgatccc gcggcgcccg cgccgttcgg cctcctggga gggctgggcc
     4561 ttctcgcctc gggactccgc cgcgagggga tcgacgaggt ctacgacctc caccagaacc
     4621 ttcgctcctc gcggctcgtc tcctcgctcg ggcaggtgcg ccgcgtcgcg ccgtccaagc
     4681 acggactccg ccgccggctg atggtgcatg cgaagtggct ccatcccgag ccgctcccgc
     4741 cgctgctcgg gacctatcgg gagatcgcgg ggctcaaccc cgcagcaccg gtgcggccct
     4801 ggctgcgcga ggcgctgggg gattccgagc gggcgcgggc gcgcgcccgg ctcggcgagg
     4861 gaggggagga acgcatcgcc ctgctcgggg tcggcgcgcg ctgggagacg aagcgctggc
     4921 cgctcgaacg cttcgtcgag ctgggggagc ggctgcatgc cgagaccggg ctttcgccgc
     4981 gctacgccgt tttgccgaag agcgcggagg cgtccgcact gcgcgcgcgc cttccggagg
     5041 cccgctacgg tgcgatcctc gagggatcct tccgcgagtg cgccgcggcc gcctccttcg
     5101 cccgcgtcat cgtctcgaac gattcggcca cgctccacct gggcgcggcc ctcggcgtcc
     5161 cggccgtcgg cattttcggg agcaccgtcc ccgccttcgg cttcgccccg tccggtccgc
     5221 gcgacgcggt ggtcgagacg gcgctctcgt gtcgcccctg ctcggtgcac gggcggcggc
     5281 gctgcccgct ggggcatcac cgctgcatgc gcgatatcgc gcccgacctc gttctggccg
     5341 cggtgcagcg ggtgctggcg ggcgccgagg ccgcgccgcc ggcggaggcc agggcgtgag
     5401 cgaccgcatc cttctcgaag ggaacgaccc gtcgatccgg gtcgccgtgg agcggcgcgc
     5461 gggaatcgcc ctcccgtggg cggccgcggg cgaagaggcg cgggccacgg tctggttctg
     5521 tcccggcgtc ccgcccgccg cggcgcgcga gctgccggcg ctccgctgga tccagacggg
     5581 ctgggcgggc gtcgaaacgt ggttcggtcg ccccgagtgg cgcgaggggg tcctcctgac
     5641 ccgtacggtc ggcgactttc cgcagcgcat cgccgagtac gtgatcggct atctcctcgc
     5701 ggacgcgctc ggcgttcccg aggcgttgcg gcagatggaa gcgcgcgcgt ggaagcgctg
     5761 gacgccgggg tcgttggccg gccgctcgct cctcgtggcg gggcacggcg cgatcggccg
     5821 ccgcgtcgcc gaggccgcgc ggggtctggg catgagctcg cggggcatcc gccgcggacc
     5881 gctgtcgtcc gaggacctcg ccctcggcgt ggaggaaccg tccgcgctcg aagcgctcct
     5941 tccggccgcc gacgtcgtcg tgaatctcct cccgctcacg accgcgacgg actccttctg
     6001 gaacgccgcg cggttcgagc gcatgaagga gggatccacg ttcgtgaacg tctcgcgcgg
     6061 cgcgacggtg gacgacgagg cgctgatcgc ggccctcgcg cggcggcgcc cggcgcgcgc
     6121 catcctcgac gtctttcgcg acgagccgct tccggccggc cacccgctgc gctcggctcc
     6181 gggcgtctgg atcacgccgc acgtcgcggg gatcgggacc tccgagccgc tcgccgccga
     6241 gttcgccgcg aactggctgc gctacaggga cggcgcgccc cttcgaaacc gggtcgatcg
     6301 ggcccgtggg tattgaggaa cgcgcggcgc ggacacgccg gtggtcccgt gggggtatcc
     6361 tggggaaagt ccttgattcc ggcgcccccc ttctcataat cggcctcgac gcgcgccgtt
     6421 ccaaaatcgc gaaaggagca tcgccatggc ggttacgacc accaaggagc cgatcgtgaa
     6481 acaggggatg atgtgggcga tccacaagcc gagcgcggcg ccgggcctct tcccgacgac
     6541 cgtgccgatc ccttcgatcg gtcccaagga cgtacgcatc cgggtgaccg cgttcgccat
     6601 ctgcggcacc gatctgcata tcgtggagtg ggatccgtgg gcgcagtcgc ggatccgccc
     6661 gccgctcgtt ccggggcacg agttcaccgg cgtcgtggaa tcggtcggcg acgaggtgtc
     6721 gctggtcaag ccgggcgacc gcgtcaccgc cgagacccac atctactgcg gcatctgcta
     6781 cgcctgccgc cgcgggctcc agcacctctg ccagaaggtg cagatcctgg gcgtggaccg
     6841 gcagggcgcg ttcgccgact acgtgtcgat cccggagcag aacgtctgga agctgcaccc
     6901 ggacatcccc gaggagatcg cggcgatcca cgatccgctc gggaacgccg tgcacaccgc
     6961 gctggccggt ccggtcgcgg ggctgcggtt cgccgtgttc ggatgcggcc cgatcgggtg
     7021 tttctcgatc ggcgtgctca aggccgccgg cgcttcgtgg gtcgcggcga tcgacaagaa
     7081 cccggggcgg ctcgagctgg ccaaggcgat gggcgccgac ttcacggtgg acgtgaacat
     7141 cgcgtcggcc tccgcggaag tgctccggga gaccgacggc cgcggggtgg acggcatgct
     7201 cgagatgtcg gggcatccgc aggcgttcaa ggacggcttc gacatgctcg ccaacggcgg
     7261 gcgcgtgtcg ctcctcggca tcccctccaa gccgctcgag atcgacgtgg cgaaggagat
     7321 cgtgttccgc ggcgccgtcg tgcaggggat caacggacgt ctcattttcg acacctggtt
     7381 ccggatggag gccctgctgc tcaccggcaa gctgaacgtg cggccggtga tcacgcacgt
     7441 gctcggctgg tcggagttcg atcgcgcggt ggagctgcag cgttccggca aggcgggcaa
     7501 gatcgtcctg aagcgggacc acccatgagc cccgctcccg aaaccatgaa gccggcgaat
     7561 ccgctcgcgt tcctcgacga ggcgctcgac ggccttcgcc gggaggggct ctaccggcac
     7621 ctgcgcctcc tctccgggga gcagaagccg cgcgcccgct tcgacgggcg cgacgtcatc
     7681 aacctctcct cgaacaacta tctcgg
//
LOCUS       DASSLZ010000086         8477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_150509, whole genome shotgun sequence.
ACCESSION   DASSLZ010000086 DASSLZ010000000
VERSION     DASSLZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150509"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2745)
                     /locus_tag="VFM00_03135"
     CDS             complement(<1..2745)
                     /locus_tag="VFM00_03135"
                     /inference="COORDINATES: protein motif:HMM:NF044450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497270.1"
                     /translation="MTSGSGRPAIAAARLVLGASLILTVTAGSALAALPVPITVQEAV
                     VAGVPGLARTAGPVSVGIPLEEAQGITSVSQLGLTGASAGQFRELARYPGGALKWVLV
                     DFQSDLTAGGSSAAVSLTNGSGNFGGSDLAADQGTSIQVSTGAATFTIRKSPFRLFDQ
                     VSVGATSLVTAGGEGVVAIDPSGVRFTSANDTGASVTIEENGPVRAVVRATGTLKNSA
                     GARLCEYLVRLHFYKGKSYARAWVSLRNARAANVSAFAFKSAEVVVPLAPGPSLRFTT
                     ATSRGTVTDALASGETMYLYQAYSTQNGIPENAYTNAPMVISGTTFAQNGVEVRKVGG
                     TIYQALSGNAADYALGWASLEDGTGRGITLGMRWMSALWPAGFELAGDGSARIEIFSK
                     RNSKTSIKFAWGAYETRELFFDFHTAAPANRNTALYEMQYPLSGRGPVAQYSAAGAVY
                     GETKLVSANDQVRWFTAHGGTSPSLANLTPGFYRYKAWGAGGGGNQMDFAFVDLIDWI
                     RTGNGGFLAEGERNSLYKADTAVRHSDGFDWTANQIDPGDEGSGVNLGTFNGRIFDFE
                     HPHWISLPIAYYMTGNELYHEAFVDYGEWKHGMADGVTPYYYKPLQIFGDGGMRGWSR
                     YYRDFALLWDITRDPRYWSDLSTMTTATLASRDTPGSGLPAGRNLDRGYMWQSWNGYV
                     TPRLVSDFMTVQIHFEAMWEGLRLMREANDPRAQGMEDYLLGLADFIYNEFYFDVGSG
                     STQFGYVYAYYLDQLNDATNPYWSEGFRPISSARALTLAYNLTGDAKYLTRAAKLLIG
                     DIQYVTVRTPSDPASESMMQTDLYRPVTGWFTVPGVTATSVGSGTYQLSWTVPPGTTG
                     YRIKAADRTIVPWLGFNQATRAYQFPPASNVAWFAAADAPGTVPAPLAPGTVQTVTVS
                     G"
     gene            complement(2726..3892)
                     /locus_tag="VFM00_03140"
     CDS             complement(2726..3892)
                     /locus_tag="VFM00_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET7497271.1"
                     /translation="MLLWLQRTSAALLAAALLVVSGEAEAALSIPSNTWVKQPTPSQV
                     GLSGFSGQFQARGWNRMMYDPLGKRTILFDGYMDASRPYTIYANALWTYDVLANRLSL
                     EKVNNWARLNGITTPLPANTTDPTPFDRHSYACIAFVPEKNEVVMWGGANSSISNDYI
                     GDTWIYSFATRAWREVTAATHPFNVFEQTATYDPNTHRFVVFGGATSSYGSGTSAWLF
                     DVNSELWEQASTPSTPPARMSQSMVYDSMRRVSYVFGGGTVYPNPGNEMWTFDASARV
                     WSQVSPQNSPPSVRRFAAMAYDTKHDLVMLWGGTNNTTALTDTWIYRPSTRQWQQLFP
                     PASPPNLDMSNEDLIYDPDNDVFMLHMNGDFWLYRYASSSDAIAPGDVSDLRIR"
     gene            4379..5389
                     /locus_tag="VFM00_03145"
     CDS             4379..5389
                     /locus_tag="VFM00_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497272.1"
                     /translation="MRDRTSRWRRAVALAVVAGAILGASASRAQSNAYNSLMAFWTAP
                     GDDGSTGQVSAYELFYDTRAPGADIGAWWNAVPFSQRVSLIPPLAAAGQPDSARVPGL
                     SQGTTYYFVIVARDEAANVSGYSNVASGTTLSCGAPTTTPASFGADVDSAGVLVSWDA
                     ATDPAALSLHLYRAVGSAPSWTLIQSLAPTAASYRDPQTAPGTTYRYRATWAGPDIQG
                     VTCEGPYSAQAVVTTPARAGAAAIAGSGQIHAYPNPASDQVQVALDVAAPSPIAARIR
                     VYDLNGHWLATVAEGTFPPGRSVVTWDRSGRTGRKLAPGYYELLGTVGNSRVRERLVL
                     LP"
     gene            5390..6955
                     /gene="xrtA"
                     /locus_tag="VFM00_03150"
     CDS             5390..6955
                     /gene="xrtA"
                     /locus_tag="VFM00_03150"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HET7497273.1"
                     /translation="MDSIRAKSGGLAPSLDLPRPRRWGLAAALFALGLAAMAAAYPAT
                     LGSFYEVWRGSDTYTHCFFVIPITAWLAWRRREDLRALTPEPFWPGLILIAGAGALWL
                     VSHVAGVLFYEQLAFALMIPALVLTLFGWRVTRTLTFPLAFLFFAVPFGEALHPALMN
                     FTARATVFGLRLTGVPVAVEGMYLTTPTGNWQVVEACSGLRFLISMFTLGCLFAYLHF
                     QRARNRILFGSLALVAPVLANGLRAYVMVLTGYLSRREIGAGFDHFAIGWTVFAIAMA
                     IFFFLGSRWRETPVPVDRAAAPSPAPSRSPRRLAVAAAAAMALALAPAAYAAWVRAPV
                     ESGPVALRAPASTGGWMPLRIPPSVWAPEFKGTDAAVRAVYEKNGGLVDCYIGFYRNQ
                     SQGRELIHYANVIAPRGESVWHVVAQRDTTIAAGGEGVPVHETLMRSPGLRYGVWHWY
                     WLPDEFTGSATHAKLIQARSRLLGRQDHAAVVILSAPYVEDPARARARLDDFTRAMLP
                     SIHRSLREADASH"
     regulatory      7141..7237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            7302..8183
                     /locus_tag="VFM00_03155"
     CDS             7302..8183
                     /locus_tag="VFM00_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET7497274.1"
                     /translation="MPDIKSTTESGAARAARVPLWVLIAIFLAGIALLSVIQPAAAHA
                     QTAADSTVLLNWTAPGDDGTVGRATTYDIRYRTVAIAGTDTTTWWNAATQVTGEPAPG
                     TAGASDSMRVRGLSPLTTYYFMIRAADEVPNWSGYSNVAVRATSGDATPPATIADLSV
                     TGTTGTSISVRWTAPGDDGTTGTAASYDVRYSTSTITSANWSSALQATGEPAPAAAGT
                     SQTFTIGGLSGSRTYYVAIKTTDDAGNVSVVSNVVNGTTSDTVAPAPVRDLSRTDALP
                     DSEMLALADTGDAADLP"
     gene            8180..>8477
                     /locus_tag="VFM00_03160"
     CDS             8180..>8477
                     /locus_tag="VFM00_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497275.1"
                     /translation="MTPHTLSNRDRRARAAVVAYAAAALTGAAALAALGPAPARADSI
                     SLTWTATGDDGTAGRATTYELRFSEQPVSGTDTTTWWNNASTAGALPPPLTSGSR"
BASE COUNT         1236 a         2986 c         2927 g         1328 t
ORIGIN      
        1 gccgctcacg gtcacggtct gcacggtgcc gggcgcgagc ggggcgggga ccgtcccggg
       61 cgcgtcggcg gccgcgaacc acgccacgtt gctcgcgggc gggaactggt aggcgcgcgt
      121 cgcctggttg aaccccagcc agggcacgat cgtccggtcc gccgccttga tgcggtagcc
      181 ggtcgtgccc gggggaacgg tccaggagag ctggtaggtg ccgctgccca cgctggtcgc
      241 ggtcacgcca ggcaccgtga accaccccgt taccgggcgg tacagatcgg tctgcatcat
      301 cgattcgctc gcgggatcgc tgggcgttcg caccgtgacg tactggatgt cgccgatcag
      361 gagcttcgcc gcccgcgtca ggtacttcgc gtcgcctgtc aggttgtagg ccagggtgag
      421 cgcccgggcg ctggagatcg gacggaagcc ttcggaccag tagggattgg tggcgtcgtt
      481 caactggtcc aggtaatagg cgtagacgta cccgaactgc gtgctcccgc tgccgacgtc
      541 gaaatagaac tcgttgtaga tgaagtcggc cagcccgagc aggtagtcct ccatcccctg
      601 cgcgcgcgga tcgttcgcct cgcgcatcag gcgcagccct tcccacatcg cctcgaagtg
      661 gatctgcacc gtcatgaagt cggagaccag gcgcggcgtg acgtagccgt tccaggactg
      721 ccacatgtag ccccggtcca ggttccgccc ggcgggaagt ccgcttccgg gagtgtcccg
      781 cgacgcgagg gtcgcggtgg tcatcgtgct gagatccgac cagtagcgcg gatcgcgcgt
      841 gatgtcccag aggagagcga agtcgcggta gtagcgcgac cacccgcgca tgcctccgtc
      901 gccgaagatc tggagcggct tgtagtagta gggcgtgacg ccgtccgcca tgccgtgttt
      961 ccactcgccg tagtcgacga acgcctcgtg gtagagctcg ttgccggtca tgtaataggc
     1021 gatcggaaga ctgatccagt ggggatgctc gaagtcgaag atccggccgt tgaacgtgcc
     1081 gagattcacg ccgctcccct cgtcgccggg atcgatctgg ttcgccgtcc agtcgaagcc
     1141 gtcggagtgg cgcacggcgg tgtcggcctt gtagagcgag ttccgttcgc cctcggcgag
     1201 gaagccgccg tttccggtgc ggatccagtc gatcaggtcc acgaacgcga agtccatctg
     1261 gttgccgccg ccgcccgcgc cccacgcctt gtagcggtag aagcccggag tcagattcgc
     1321 cagggagggg ctggtgccgc cgtgggcggt gaaccagcgc acctggtcgt tcgccgaaac
     1381 cagcttcgtc tcaccgtaca cggcgccggc cgcggagtac tgcgccaccg ggccgcgacc
     1441 cgagagcggg tactgcatct cgtagagcgc ggtgtttcga ttggccggcg ccgcggtatg
     1501 gaagtcgaag aagagctccc gcgtctcgta ggcgccccac gcgaacttga tggaggtctt
     1561 gctgttccgc ttgctgaaga tctcgatccg cgcgctgccg tctccggcca gctcgaatcc
     1621 cgccggccag agcgcgctca tccagcgcat gccgagggtg atcccgcggc cggtgccgtc
     1681 ctcgagcgag gcccatccca gcgcgtagtc ggcggcattg ccggacaggg cctggtagat
     1741 cgtaccgccc accttccgga cctcgacgcc gttctgcgcg aacgtcgtcc cggagatcac
     1801 catgggcgcg ttggtgtacg cgttctccgg gatgccgttc tgcgtcgagt acgcctggta
     1861 gaggtacatg gtctcgcccg aagccagggc gtcggtcacc gtcccgcgcg aggtcgccgt
     1921 cgtgaagcgc aacgacggcc cgggggcgag gggaacgacg acctcggcgc tcttgaacgc
     1981 gaaggcggag acgttcgcgg cacgggcgtt ccggagcgag acccacgccc gcgcgtacga
     2041 ctttcccttg tagaagtgca gccggacgag gtactcgcag agacgcgcgc ccgcgctgtt
     2101 cttcagggtg ccggtggcgc gcaccaccgc gcgcacggga ccgttctcct cgatcgtgac
     2161 cgaggcgccg gtgtcgttcg cggaggtgaa ccgcacgccc gacggatcga tcgcgacgac
     2221 cccctccccc cccgccgtga cgagggacgt ggcgccgacc gagacctggt cgaacagccg
     2281 gaaggggctc ttccggatgg tgaacgtcgc cgcaccggtc gagacctgga tggacgtgcc
     2341 ctggtcggcc gcgagatcgg agccgccaaa attcccggag ccgttcgtga gcgacaccgc
     2401 ggcgctcgag ccgcccgcgg tgagatcgct ctggaagtcg acgagaaccc acttgagggc
     2461 gccgcccgga tagcgcgcga gctcgcggaa ctggcccgcg gaggcgccgg tgagcccaag
     2521 ctgcgacacg ctggtgattc cctgggcttc ttcgagcggg atccctacgc tgaccggacc
     2581 cgccgtgcgc gccagccccg gaacaccggc cacgaccgct tcctgcacgg tgatcggaac
     2641 ggggagcgcg gcgagcgccg agcccgccgt cacggtcagg atcagggacg cgcccaggac
     2701 gagacgcgcc gcggcgatcg cgggcctacc ggatccggag gtcactcacg tccccggggg
     2761 cgatggcgtc gctgctcgat gcgtagcggt agagccagaa gtctccgttc atgtgcagca
     2821 tgaagacgtc gttgtccgga tcgtagatga gatcctcgtt gctcatgtcc aggttcggcg
     2881 gggacgcggg agggaagagc tgctgccact gccgcgtcga cggccgatag atccaggtgt
     2941 cggtgagcgc cgtcgtgttg ttggtgccgc cccagagcat gacgaggtcg tgcttcgtgt
     3001 cgtacgccat ggccgcgaag cgccgtaccg acggggggct gttctgcggg gacacctgcg
     3061 accagacgcg ggccgaggcg tcgaaggtcc acatctcgtt tccggggttc ggatagacgg
     3121 tgccgccgcc gaacacgtag gagactcgcc gcatggaatc gtagaccatc gactgggaca
     3181 tccgggccgg aggcgtgctc ggcgtggagg cctgctccca cagctccgag ttcacgtcga
     3241 agagccaggc cgaggtcccg ctcccgtagg aggaggtggc gccgccgaac acgacgaagc
     3301 ggtgcgtgtt cggatcgtag gtggcggtct gctcgaacac gttgaagggg tgcgtcgctg
     3361 ccgtgacctc gcgccaggcc ctcgtcgcga aactgtagat ccacgtgtcg ccgatgtagt
     3421 cgttcgagat actgctgttc gcgccgcccc acatcacgac ctcgttcttc tcggggacga
     3481 atgcgatgca ggcgtaggag tggcggtcga agggggtcgg atcggtcgta ttcgccggga
     3541 ggggtgtggt gatgccgttg aggcgcgccc agttgttcac cttctccagg gaaagccggt
     3601 tcgcgagcac gtcgtaggtc cagagcgcgt tcgcgtagat ggtgtagggg cgcgacgcgt
     3661 ccatgtatcc gtcgaagagg atcgtcctct tgcccagcgg gtcgtacatc atgcggttcc
     3721 aaccgcgggc ctggaactgg ccggagaatc cggacagccc gacctggctg ggggtcggct
     3781 gcttcaccca tgtgttggat ggaatggaga gggcggcctc ggcctcgccc gaaacgacca
     3841 ggagcgcggc cgcgaggagc gcggcggacg ttcgctgcag ccacaggagc acagttccac
     3901 cctccggggc cggcgtttgc ggcggccggc gccgatgctg attctcgatg taggttcgcg
     3961 gtgacggaga aggctttccc ccgcacacga acaagcagct atcgtaccat cgttcgggag
     4021 cgtgccgtgg cgtgatcggc gcggcgccgc attcgattgg gcccgaccca aatggcatcc
     4081 cggccgcgcc gcaaaccgca actctcgacc acagctacgg tgctatctct tgcacgccgc
     4141 gcgtgcgaga cgccaccggc ggcgggcgtg gcttcggggc cggtccagcc cgcaacctcg
     4201 cgattcgctc ccgttcgcgt accgatccgt actccgggcg accgaagcgg gttccgcgcg
     4261 aacatggaac cgagcgtgcg atttgcctca taatggcttt cttgccttcc gatggtacga
     4321 acgcggcggg gtatgcccga agggtcgacg gccaccccgc gaccggagga gacaccacat
     4381 gagggaccgg acatcgagat ggcgtcgcgc ggtcgcgctc gccgtggtgg cgggcgccat
     4441 cctgggcgct tccgcctcgc gcgcgcagag caacgcctac aactccctca tggcgttctg
     4501 gacggccccc ggggacgacg gcagcacggg gcaggtgagc gcctacgagc tcttctatga
     4561 cacgcgcgcg cccggcgccg acatcggcgc ctggtggaac gcggttccgt tctcgcagcg
     4621 cgtgagcctc atcccgccgc tcgccgcggc cggccagccc gattcggcgc gcgtgccggg
     4681 cttgagccaa ggcaccacgt attacttcgt gatcgtcgcg cgggacgagg ccgcgaacgt
     4741 ctccggatac tccaacgtcg cctcggggac cacgctttcg tgcggggcgc ccacgacgac
     4801 gccggcctcg ttcggagccg acgtggactc ggccggggtc ctggtctcct gggacgcggc
     4861 gaccgatccc gccgcgctct ccctccactt gtatcgcgcc gtcgggtccg cgccctcgtg
     4921 gaccctgatc cagtcgctcg ctcccacggc ggcgagctac cgcgatccgc agaccgcgcc
     4981 gggtacgacg taccgctatc gcgcgacctg ggccgggccc gacatccagg gcgtcacctg
     5041 cgaaggcccc tattcggcgc aggccgtcgt cacgaccccg gcgcgcgccg gagccgcggc
     5101 gatcgcgggg agcggacaga tccatgcgta tcccaatccc gcttccgatc aggttcaggt
     5161 cgctctcgac gtcgccgcgc cgtcgccgat cgccgcgcgg atccgggtct acgacctgaa
     5221 cggtcactgg ctcgccacgg tcgccgaagg gacgttcccg ccggggcgca gcgtcgtcac
     5281 ctgggatcgc tcgggccgga ccggccggaa gctggccccg ggctactatg agctcctggg
     5341 aacggtcgga aacagccgcg tgcgcgagcg cctcgtcctt ctcccctagg tggattccat
     5401 tcgagccaag tccggcggcc tggccccgtc tctcgatctc ccgcgcccga ggcgctgggg
     5461 gctggccgcc gcgctgttcg cgctcggact ggccgcgatg gcggcggcct atcccgcgac
     5521 gctcggatcg ttctacgagg tgtggcgcgg ctccgacacg tacacgcact gcttcttcgt
     5581 gatcccgatc acggcgtggc tcgcctggcg ccggcgcgag gacctgcggg ctctgacccc
     5641 ggagccgttc tggcccgggc tcatcctcat cgcgggcgcg ggcgcgctct ggctggtgtc
     5701 ccacgtcgcg ggcgtcctct tctacgagca gctcgccttc gcgctgatga tccccgctct
     5761 cgtgctcacg ctgttcggat ggcgcgtgac gcgcacgctc acgttcccgc tcgcgttcct
     5821 cttcttcgcg gtccccttcg gggaggcgct ccacccggcg ctcatgaact tcacggcccg
     5881 ggcgacggta ttcggcctgc gtctcaccgg tgtgcccgtg gcggtggagg ggatgtacct
     5941 gacgacgccc acggggaact ggcaggtggt ggaagcctgc agcggcctcc gatttctcat
     6001 ctcgatgttc acgctcggct gcctcttcgc ctacctgcac ttccagcgcg ctcgaaaccg
     6061 catcctcttc ggctccctgg ccctcgtggc gccggttctc gcgaacggac tccgcgccta
     6121 cgtcatggtg ctgaccgggt acctgagccg ccgcgagatc ggcgccgggt tcgaccactt
     6181 cgcgatcggc tggacggtgt tcgcgatcgc gatggcgatc ttcttcttcc tggggagccg
     6241 ctggcgcgaa acgccggttc ccgtggaccg agcggcggcg ccctcgcccg cgccgagccg
     6301 ctcgccgcgc cggctggcgg tggcggccgc ggcggcgatg gccctcgcgc tcgcgccggc
     6361 ggcgtacgcg gcctgggtcc gggcgccggt cgaatcgggc cccgtcgcgc tccgcgctcc
     6421 cgcgtcgacc ggaggctgga tgccgctccg gattcctcct tcggtctggg cgccggagtt
     6481 caagggcacg gacgcggcgg tgcgcgccgt ctacgagaag aacgggggcc tggtcgactg
     6541 ctacatcggc ttctaccgaa atcaatcgca gggacgggag ctgatccact acgcgaacgt
     6601 gatcgcgccc cgcggggaat cggtgtggca cgtggtggcg cagcgcgaca cgacgatcgc
     6661 cgcgggcggg gaaggggtcc ccgtgcacga gaccctcatg cgttctcccg gacttcgcta
     6721 cggcgtgtgg cactggtact ggctgcccga tgagttcacg gggagcgcca cgcacgccaa
     6781 gctgatccag gcgcgctcga ggctcctcgg ccggcaggac cacgcggccg tcgtgatcct
     6841 ctcggcgccc tatgtggagg accccgcgcg cgcccgcgcg cggctcgacg acttcacccg
     6901 cgccatgctt ccctcgatcc atcgctccct gcgcgaggcc gacgcctccc actaggttgc
     6961 atcctgaaat gcccgcgggt gcaaaacccc ggatgcatcc tgcacggcgc ggccggccgg
     7021 catcgcggtt cgcagccatc cgcccgccta tccccgggcg cccgccgtgc acgacgcaag
     7081 ccgcctcctg gcaccgaacc tgctcggagc ccgggtacgc ggcagcatcc atatgctaat
     7141 ccatgctcag agcaaaccgt ccgaaagggc ggggcgcaga gtcacggggc ttccgatcgc
     7201 ggggtctccc tcgatccacg ccggccgggc cgccatggat ccgaagcgga tcgtggcgga
     7261 cctcgacatc cggcgagggc cgtcgaggag ggagacaacg catgcctgac atcaagtcga
     7321 cgaccgaatc cggcgccgcg agagccgcga gggtcccgct ctgggtcctc atcgcgatct
     7381 tcctcgcggg catcgcgctg ctgtcggtga tccagccggc ggccgcgcac gcccagaccg
     7441 ccgccgactc gacggtgctc ctgaactgga ccgcgcccgg cgacgacggc accgtcggcc
     7501 gcgccacgac ctacgacatc cgctaccgga cggtcgcgat cgcgggcacc gacacgacga
     7561 cgtggtggaa cgccgcgacc caggtgaccg gcgagccggc ccccggcacg gcgggggcgt
     7621 ccgattcgat gcgcgtccgc ggcctctcgc cgctcaccac ctactacttc atgattcgcg
     7681 ccgcggacga ggtgccgaac tggtccggct actcgaacgt cgccgtgcgc gcgaccagcg
     7741 gcgacgccac ccctccggcc acgatcgccg acctcagcgt gaccgggacc accggcacgt
     7801 cgatcagcgt ccgctggacc gcgccgggcg acgacggcac gaccggcacc gcggcgagct
     7861 acgacgtgcg ctactccacg tcgacgatca ccagcgccaa ctggagcagc gcgctccagg
     7921 cgacgggcga gccggctccg gcggcggcgg gcacgtcgca gacgttcacg atcggcggcc
     7981 tgagcggcag ccgcacctac tacgtcgcga tcaagacgac cgacgacgca ggaaacgtct
     8041 cggtggtctc gaacgtcgtg aacggcacca cctcggacac cgtcgctccg gcgcccgtgc
     8101 gcgacctgtc gcgcaccgac gcgctgcccg actcggagat gctggcgttg gccgacaccg
     8161 gcgacgccgc agaccttccg tgacgcccca cacgctctcg aaccgtgacc gccgcgcacg
     8221 cgcggcggtc gtcgcgtacg ccgcggccgc cctgacgggc gccgcggcgc tcgccgcgct
     8281 cggtcccgcg ccggcccgcg ccgactccat ttccctcacc tggacggcga cgggcgacga
     8341 cggcaccgcc ggacgcgcca cgacgtacga gctccgcttc tcggagcagc cggtctcggg
     8401 caccgacacg acgacctggt ggaacaacgc ctccaccgcc ggcgcgcttc cgccgccgct
     8461 gacgtcggga tcgcgcg
//
LOCUS       DASSLZ010000087         8749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_918219, whole genome shotgun sequence.
ACCESSION   DASSLZ010000087 DASSLZ010000000
VERSION     DASSLZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918219"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFM00_03165"
     CDS             complement(<1..228)
                     /locus_tag="VFM00_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7497276.1"
                     /translation="MSQDRSRSRVLVVDDEPDLVRILEFGLKAAGYAVETASDGQEGL
                     KKARESKPDIILLDLMLPKLDGYKVCRLLKFD"
     gene            complement(240..1586)
                     /locus_tag="VFM00_03170"
     CDS             complement(240..1586)
                     /locus_tag="VFM00_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HET7497277.1"
                     /translation="MKRMLAILTLAAGLAAVATPWSAPAEPQGRATFSFKGARAQDAL
                     RLIAAQFGVNIVIGKDVPGTLTANLSNVTFAEAVSYVAEATGADYRLEWNVLLVNPAG
                     STSRLFRLRYLDPTAAADVVRKMLSEKGTVEPFSGRASTVPGASGEGLLPETSNGSRE
                     NAVIVTDTPARLERIAAVLDEMDARPRQVAITAKLVETTLGKDEKLGVDWQLRASAAG
                     ATLPTTFPFPKTAGSGDFTPTPNPNNQVGGGGPAFPPGQTFPYPTPADYVFGKLSFQE
                     FSVAMDILRQTANTNLVSAPTITTLDNNEAEIVVGTVVPVARYERLKETGTMEISGYD
                     EKKIGVRLLVTPHVADDSTMILSVSPEISEIVEYRGQFNERPVTSTRSATTQVVVKNG
                     ETLAIGGLIKTVDLQIVRKVPILGDVPLLGALFTHKTIQKQKVDLMIFVTPHLLGK"
     gene            complement(1583..2080)
                     /locus_tag="VFM00_03175"
     CDS             complement(1583..2080)
                     /locus_tag="VFM00_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497278.1"
                     /translation="MSWLREQARRGMNPRTYVLLGLLVAALVTVQSLDGQSKRARIRA
                     RAAGEAPPPAQLAASAPKQGPSTPTEPGWGNDPFRRRFGPEGAPAATTPSARREAPPP
                     SGAGLYLQGVMNGPSGRTALINGEVYREGERVGSREILQIGARTVMLMDKGTVTTLTL
                     KGDAP"
     gene            complement(2095..2667)
                     /gene="pilO"
                     /locus_tag="VFM00_03180"
     CDS             complement(2095..2667)
                     /gene="pilO"
                     /locus_tag="VFM00_03180"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET7497279.1"
                     /translation="MESPIRQPYLGHLVIAGVLLASLGVVRAVYVDPREREAKQLRTE
                     RGRLEADLADLQHGLQDLDAWQRVHPGEDASRFSARHALPSRAMVAAFLRDVTPIADR
                     WGVKTELIQPAGTPVDVTTTDAAGRTETFRKAELRFRLYATYRSLGEYLREVEAMDQL
                     VVVRSVAIRADAPKETELSADVAIWLYGTF"
     gene            complement(2676..4319)
                     /gene="pilM"
                     /locus_tag="VFM00_03185"
     CDS             complement(2676..4319)
                     /gene="pilM"
                     /locus_tag="VFM00_03185"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HET7497280.1"
                     /translation="MGGRVGVDVSDASVHVVRLRRGAGGLAVEGLSAAVPAGGSGAAG
                     VEALRGLVEAAGLKGAPAAATITGNDVVIRRITMPLMKRADLVAALRLECRKHVAGPI
                     EDAEIRFEPLGRSGPDGPPGLDLLVAVAPRRKVEEARELLEQAGLKAACVTPRPVALR
                     ALLRAAGETRADEAAAYLEVGVAESHVTVLKGREIRFARDLGVGSASFAEALRSIVVP
                     GQGTVELSAEEAESLVRAHGIPDGAQEAASAGPIPLTAVSVMLRPVLERLVRELWNSF
                     DYVNEQFLGESVSRVVLLGEGARVRNLPEYLAGVLKIPVAGADLFERAARAERAPRGE
                     ARPASELALGLCLPDAGAMNFLAPPDAGTAYRLASAVPQRVAAAVAAVLLLSVSLPAE
                     MAVVGERQRVASLRGSLEEVRPRAEALRRFRAAREEETRLHDLLARLSGGQVLWSYVL
                     RDLSWRVTDNARLTTLEVLEPGEPATAAGAAQPAGVSGREVRLSGLLRTDRERPEKVL
                     GELMDSLSRSPVFDQVRLEGCQTVTPTLSSFTVTVRIAE"
     gene            complement(4331..5794)
                     /locus_tag="VFM00_03190"
     CDS             complement(4331..5794)
                     /locus_tag="VFM00_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497281.1"
                     /translation="MIGRPGVARGNPAADPEAGFSVVLAVASILILLLLGLAMVSMVV
                     EDSDLSVGHVRSNQAFYLAHAGVEYGVKKLAANPSWTGLPMPGKPVGAGAFWVAPPDT
                     VDENGAALPPGQKRIVATGIVGDASRAIQVHVASGGISTLAGNGTMGYAGDGGAATSA
                     NLKNPEGVTVATNGDVYLCDSDNHVIRRVAAATGIITTVAGNGNPGNTGDGGLATAAK
                     LKFPEDVTVAANGDLYIADTGNHVIRKVAAASGVIVTVAGNGSPGFSGDGGVATAARL
                     NSPRGIQVAANGDLYIGDRTNNRIRKVTAATGTITTAAGTGTAGYSGDGGPATAAKLR
                     APQGLHLASNGDLYVADAGNHVVRKISAAGIISTFAGTGTSGYSGDGGLATAARLNAP
                     EAVHLAPTGDAYVADTGNHVIRKVQAGSQAISTIAGTGAAGFGGDGGAATSAQLDTPR
                     GIAVASTGVFYIGDKNNQRVRKVGGALAVTAWIEARR"
     gene            complement(5791..6354)
                     /locus_tag="VFM00_03195"
     CDS             complement(5791..6354)
                     /locus_tag="VFM00_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7497282.1"
                     /translation="MVHQLLTRAVPRGRRGGLAPPDAAGFTLAEVTIVIVVVSVAAFV
                     FTGMFLEAIRSYQFEDAEKGMLQEARYAEERISRELRRVRGDTYVTQATPRAITFVDR
                     DSAVVGFSWSGVKGDPLLYTKNGSSQTLATGVDSLAFAYWKSDGTAAAPVVAPAATDI
                     WRVSVHLRLAKGSQSVSTVTAALVRPM"
     gene            complement(6336..6758)
                     /locus_tag="VFM00_03200"
     CDS             complement(6336..6758)
                     /locus_tag="VFM00_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497283.1"
                     /translation="MRRGAAAAGEGLARDAGFTLPEVVLILVIAAVAVLPLGMLFANT
                     SIRSGEARSATVMAQLAQAKMEEIAADKSSPSRGFSYLTAANYPAENPVTAFPGYSRT
                     VSFAPDSVYDGVTFRTVSVTVAHASVPAVTLTTWFTSY"
     gene            complement(6755..7255)
                     /locus_tag="VFM00_03205"
     CDS             complement(6755..7255)
                     /locus_tag="VFM00_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7497284.1"
                     /translation="MSRARGFTLVELALAITIVAILAWVAYPKVAAMGEIRLDAAARR
                     LAGDVRYAQSRSINTRTVHGIYFQPSLSRYTVYALTPATPVADPADRARPLRVDYASR
                     VEFRGVSIASVSFGSGRALSFDYYGVPRDSGGTDLASAGRVILSYQGLSDTVLIAPGT
                     GTVTVR"
     gene            complement(7252..>8749)
                     /locus_tag="VFM00_03210"
     CDS             complement(7252..>8749)
                     /locus_tag="VFM00_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497285.1"
                     /translation="RRGAGAPRIARGMGWAVLGFSLTLVIPVRWSAGPSFAWTDVRGL
                     SGLADFLLRRTYGGLAQNPFRLDRAFDEVTGMGGIAIASLGWLGAALAAAGLFARRAR
                     SLAGALALAAATIPAALIALIAFTPDAEHLAQVTPFLAPLVAAGALFAGAGAQSVAGF
                     AARLRARRAAGIGGSLGSAGVRRALASAALVAAALALVPGRYAAADRSRFTLAERYGK
                     DLLAEAPRGATLVLDGDNETFLAAYASRVEGCRPDVTLRHRRGWIFGDPCGLRSKPRA
                     EWAALAHQDEMERIRRGTETLCYAVPPADLIRAGVSFRQRGLLYAAAPSGSDAAGWDP
                     PAGWRRSSDLLEGHPERYDYVTRKMAVSYSDAAARRLWARGQVAEALPWFLDAARVGY
                     DFPGARLNAATALAAAGEPDRALGELLAACRLAPYDPEPAARLAVFLASAGRHRDAAL
                     WFERAFRAEPLAAIAADAARAWSLAGEPARAEEWGRRAASFGVAGGEG"
BASE COUNT         1223 a         3288 c         3043 g         1195 t
ORIGIN      
        1 gtcgaacttg aggagccggc agaccttgta cccgtccagc ttcggcagca tgaggtcgag
       61 gaggatgatg tccggcttcg actcgcgcgc cttcttcagc ccctcctgcc cgtccgaggc
      121 ggtctccacg gcataaccgg ccgctttcag gccgaactcg agaatgcgca ccagatccgg
      181 ctcgtcatcc acgacgagca cgcgcgagcg ggagcgatcc tggctcatcg gtcgtcctcc
      241 tactttccca gcaggtgcgg ggtcacgaag atcatgagat ccactttctg cttctgaatc
      301 gtcttgtgcg tgaagagcgc ccccaggagg ggcacgtctc ccaggatcgg cactttccgg
      361 acgatctgga ggtccaccgt cttgatcagc cccccgatgg ccagggtctc cccgttcttg
      421 accaccacct gggtcgtggc cgagcgggtc gaggtgacgg gccgctcgtt gaactggccc
      481 cggtactcca cgatctcgct gatctcgggg ctcacgctca ggatcatggt ggagtcgtcg
      541 gccacgtggg gcgtcaccaa cagccgcacg ccgatcttct tctcgtcgta gccgctgatc
      601 tccatggtgc cggtctcctt cagccgctcg tagcgggcca cgggaacgac cgtccccacg
      661 acgatctcag cctcgttgtt gtcgagggtc gtgatcgtcg gggcggagac caggttcgtg
      721 ttggccgtct ggcgcaggat gtccatcgcc accgagaact cctggaacga gagcttgccg
      781 aagacgtagt cggccggcgt cggatagggg aaggtctgcc cgggcgggaa cgcgggcccg
      841 ccgccgccca cctggttgtt cggattgggc gtgggcgtga agtcccccga gcccgcggtc
      901 ttcgggaagg gaaacgtggt cggcagggtc gcgccggcgg cgctcgcccg gagctgccag
      961 tccaccccca gcttctcatc cttgcccagg gtcgtctcga ccagcttcgc cgtgatcgcc
     1021 acctggcgcg gccgcgcgtc catctcgtcg aggaccgcgg cgatccgttc caaccgcgcc
     1081 ggcgtgtcgg tcacgatgac cgcgttctcg cgggatccgt tcgacgtctc cggaagaagt
     1141 ccttcgccgc tcgcgccggg aaccgtggag gcgcgtccgg agaagggctc gacggtgccc
     1201 ttctcgctca gcatcttccg cacgacgtcg gcggcggcgg tgggatcgag atagcggagc
     1261 cggaagagcc gcgacgtcga tccggcgggg ttgacgagca gcacgttcca ctccagccgg
     1321 tagtcggcgc cggtcgcttc tgccacgtag gaaaccgcct cggcgaacgt cacgttcgag
     1381 aggttggccg tgagcgtgcc cggcacgtcc ttaccgatca cgatgttcac accgaactgc
     1441 gccgcgatca gccggagcgc gtcctgcgcc cgggccccct tgaagctgaa cgtcgccctg
     1501 ccctgcggct ccgccggcgc gctccagggc gtcgcgactg cggcgagccc ggcggccagc
     1561 gtcaggatgg cgagcatccg cttcatggtg cgtctccttt gagggtgagc gtcgtcacgg
     1621 ttcccttgtc catcagcatc accgtgcgcg ccccgatctg gagaatctcg cgggagccga
     1681 cccgctcccc ttcgcggtag acctcgccgt tgatcagcgc ggtccgcccc gacggtccgt
     1741 tcatcacgcc ctgcagatac agccccgccc ccgagggcgg cggcgcttcg cggcgcgcgg
     1801 acggcgtcgt ggccgccggc gccccctcgg gcccgaagcg ccgccggaag ggatcgtttc
     1861 cccaccccgg ctcggtcggc gtcgaggggc cctgcttcgg agccgacgcg gcgagctgcg
     1921 ccggcggcgg tgcttcgccc gcggcccgcg cgcgaatccg cgcccgcttc gactgcccgt
     1981 ccagcgactg cacggtgacg agtgccgcga cgagcagtcc gaggagcacg taggtcctcg
     2041 gattcatccc ccgcctcgcc tgctcgcgca gccagctcat gcccgttccg ttccctagaa
     2101 ggtgccgtag agccagatcg cgacgtccgc cgagagctcc gtctccttcg gcgcgtccgc
     2161 gcgaatggcc accgagcgca ccaccaccag ctgatccatc gcttccacct cgcgcaggta
     2221 ctcgcccagc gagcggtacg tggcgtagag ccggaaccgg agctccgcct tgcggaaggt
     2281 ctccgtccgg ccggcggcgt cggtggtggt gacgtcgacc ggcgtgcccg cgggctggat
     2341 cagctccgtc ttcacccccc agcggtcggc gatcggcgtc acgtcgcgca ggaaggcggc
     2401 gaccatcgcg cgcgacggca gggcgtgccg cgccgagaag cgcgacgcgt cctcgcccgg
     2461 gtggacgcgc tgccaagcgt ccaggtcttg aagcccgtgc tggagatcgg ccagatcggc
     2521 ctccagccgt ccgcgctccg tccggagctg cttcgcctcg cgctcccggg gatccacgta
     2581 gaccgcgcgc accacgccca gggacgcgag aagaaccccc gcgatcacca ggtgccccag
     2641 gtacggctgc cggatcggcg actccacggg tctccctact ccgcgatccg cacggtcacg
     2701 gtgaagctgg agagcgtcgg cgtcacggtc tggcatccct ccaaacggac ctggtcgaat
     2761 accggcgagc gggacagcga atccatcagc tcgcccagga ccttctcggg ccgctcgcga
     2821 tccgtgcgca gcagtcccga gaggcgcacc tcacggccgc tcacccccgc cggctgggcc
     2881 gcccccgcgg ccgtcgccgg ttccccaggt tccagtacct cgagcgtcgt gaggcgcgcg
     2941 ttgtccgtga ctctccagct cagatcgcgc agcacgtacg accagagcac ctgccccccc
     3001 gacaggcggg ccagcaggtc gtgcaggcgc gtctcctctt cccgcgcggc gcggaagcgc
     3061 cggagcgcct cggcgcgcgg ccggacctcc tccaggctcc cccggagcga ggcgacccgc
     3121 tgccgctcgc ccacgaccgc catctccgcg ggaagcgaga ccgacaacag aagcaccgcc
     3181 gcgaccgccg cggcgacccg ctggggcacc gcgctggcca ggcggtacgc cgtgccggca
     3241 tccggcggcg cgaggaagtt catcgcgccc gcgtcgggca ggcagagtcc cagcgccagt
     3301 tccgacgcgg ggcgcgcctc gccgcgcggg gcccgctcgg cccttgccgc ccgttcgaac
     3361 agatcggccc cggcgaccgg gatcttgaga acgccggcca ggtactccgg cagattccgg
     3421 acgcgcgccc cctcgcccag gagtacgacc cgcgaaaccg attcgcccag gaactgctcg
     3481 ttcacgtagt cgaaggaatt ccagagctcc cgcaccaggc gctccagcac gggccggagc
     3541 atgaccgaca cggccgtgag cggaatcggg ccggcgctcg ccgcctcctg ggcgccgtcg
     3601 gggatgccgt gcgcgcgcac cagcgattcc gcctcttcgg cgctcagctc caccgttccc
     3661 tggccgggca ccacgatcga gcggagggcc tcggcgaacg acgcgctccc caccccgagg
     3721 tcgcgcgcga agcggatctc ccgccccttg agaacggtga cgtggctctc ggccacgccg
     3781 acttcgagat acgcggcggc ttcgtcggca cgcgtctccc cggccgcgcg gagcagcgcg
     3841 cggagcgcca ccggtcgcgg cgtcacgcag gcggccttga gcccggcctg ctcgaggagc
     3901 tcccgcgcct cctccacctt tcgccgcggc gccacggcca cgaggagatc gagccccgga
     3961 gggccgtccg ggcccgagcg tccgagcggc tcgaagcgga tctcggcatc ttcgatcggg
     4021 ccggccacgt gcttgcggca ttcgaggcgg agggcggcca cgagatcggc gcgcttcatc
     4081 aggggcatcg tgatccggcg gatcacgacg tcgttcccgg tgatcgtcgc cgccgccggc
     4141 gcgcccttca ggcccgccgc ctcgaccagg cctcggagcg cctccacgcc ggccgcgccc
     4201 gacccgcctg ccggaaccgc ggcggagagc ccctcgacgg ccagcccgcc cgcgccgcgg
     4261 cggagccgca cgacgtggac cgaagcgtcg ctcacgtcca cgccgacccg accgcccatg
     4321 agggctcctc tcatcgccgc gcctcgatcc aggcggtgac ggcgagagcg ccgccaacct
     4381 tccggacgcg ctgattgttc ttgtcgccga tgtagaagac tcccgtggac gcgaccgcga
     4441 tgccgcgcgg cgtatcgagc tgggccgacg tggccgcccc gccgtccccg ccgaagcccg
     4501 cggcgcctgt ccccgcaatc gtgctgatcg cctggctccc cgcctgcacc ttgcggatga
     4561 cgtggttgcc ggtgtccgcc acgtaggcgt cgcccgtcgg ggccaggtgc accgcttccg
     4621 gcgcgttcag ccgcgccgcg gtggccagcc cgccgtcccc ggagtagccc gacgtccccg
     4681 ttccggcgaa cgtggagatg attcccgccg cgctgatctt ccggacgacg tggttccccg
     4741 cgtcggcgac gtagagatcg ccgttggaag cgagatggag cccctggggg gccctcagct
     4801 tggccgcggt tgccggcccg ccgtctccgg agtagcccgc cgtgccggtg cccgccgccg
     4861 tggtgatcgt cccggtggcc gcggtcacct tgcggatccg gttgttcgtg cggtcgccga
     4921 tgtacagatc gccgttcgcc gcgacctgga ttccgcgcgg cgagttgagc cgcgccgccg
     4981 tggccacgcc gccgtcgccg gagaagccgg gcgagccgtt gcccgcgacg gtgacgatca
     5041 cgccggaagc ggcggccact ttccggatta cgtgatttcc ggtgtcggcg atgtagaggt
     5101 ccccgttggc ggcgacggtc acgtcctcgg ggaacttgag cttggccgcg gtggccagcc
     5161 cgccgtcgcc ggtgttgccc gggtttccgt tgcccgcgac ggtcgtgatg atccccgtgg
     5221 cggcggccac tctccggatc acgtggttgt cggaatcgca gaggtagacg tcgccgttgg
     5281 tggcgaccgt caccccctcg gggttcttga ggttcgccga ggtggcggcg ccgccgtcgc
     5341 cggcgtagcc catcgtgccg ttgcccgcta aggtcgagat cccgcccgag gcgacgtgca
     5401 cctggatcgc gcgcgacgcg tccccgacga ttccggtcgc gacgatccgc ttctgccccg
     5461 gggggagcgc cgcgccgttc tcatccaccg tgtcgggcgg agccacccag aacgctcccg
     5521 cgccgaccgg cttccccggc atcggaagcc cggtccagga gggattggcc gccagcttct
     5581 tcaccccgta ctccaccccc gcgtgcgcca ggtaaaaggc ctggttcgac cgcacgtggc
     5641 cgaccgagag gtccgagtcc tcgaccacca tggagaccat ggcgaggccc aggaggagca
     5701 ggatcaggat cgaggcgacc gcgagcacga ccgagaatcc cgcctcggga tccgccgccg
     5761 gattcccgcg tgccacgccc ggccggccga tcacatcggc ctcacgagcg ccgccgtcac
     5821 ggtgctcacc gactgcgatc ccttggccag gcgcaggtgc accgagaccc gccagatgtc
     5881 ggtcgccgcg ggcgccacga ccggcgccgc ggcggtgccg tccgacttcc agtaggcgaa
     5941 cgccagcgag tccacgccgg tggccagcgt ctgcgacgag ccgttcttcg tgtagagcag
     6001 cggatccccc ttcacgccgg accaggagaa gccgacgacc gccgagtcgc ggtccacgaa
     6061 cgtgatcgcg cgcggcgtcg cctgcgtgac gtacgtgtcg ccccgaacgc ggcgaagctc
     6121 ccggctgatg cgctcctcgg cataccgcgc ctcctgcagc atccccttct ccgcgtcttc
     6181 gaactggtag ctccggatcg cctcgagaaa cattcccgtg aacacgaacg cggcgaccga
     6241 caccaccacg atgacgatcg tcacctccgc cagcgtgaat ccggccgcgt ccggcggcgc
     6301 gagcccgccg cgccggccgc gaggcaccgc ccgcgtcagt agctggtgaa ccatgtggtc
     6361 agcgtcacgg ccggcacgct cgcgtgcgcg accgtcacgc tcaccgtgcg gaacgtgacg
     6421 ccgtcgtaga ccgagtcggg cgcgaacgac accgtgcgcg agtacccggg gaaggccgtc
     6481 accgggttct cggccggata gttggcggcc gtcaggtagg agaagccgcg cgagggagag
     6541 ctcttgtcgg cggcgatctc ctccatcttc gcctgagcga gctgcgccat gacggtggcg
     6601 cttcgcgcct cgcccgagcg gatcgacgtg ttcgcgaaga gcatcccgag cgggagcacg
     6661 gccaccgccg cgatgaccag gatcaggacg acctcgggca gcgtgaatcc ggcgtcgcgc
     6721 gccaggccct cgccggcggc cgccgcaccg cgcctcatcg gaccgtcacc gtccccgtgc
     6781 cgggagcgat cagcacggtg tcggagagtc cctggtacga caggatgacg cgcccggccg
     6841 aagcgaggtc ggttcccccg ctgtcgcgcg gcacgccgta gtagtcgaac gagagcgcgc
     6901 ggccggaccc gaacgagacg gacgcgatcg acacgccgcg aaactccacc cggctcgcgt
     6961 aatccacgcg gagcggccgc gcgcggtcgg ccgggtcggc gaccggcgtg gccggcgtca
     7021 gggcgtagac cgtgtagcgc gacagcgagg gctggaagta gatcccgtgc accgtgcgcg
     7081 tgttgatcga gcggctctgc gcataccgga cgtcccccgc caggcggcgc gcggcggcgt
     7141 ccaggcggat ctcccccatc gccgcgacct tgggataggc gacccaggcg aggatcgcga
     7201 cgatggtgat cgcgagcgcg agctccacca gcgtgaatcc gcgcgcgcgg ctcatccctc
     7261 ccccccggcc actccgaacg acgccgcgcg gcgtccccac tcctcggcgc gcgcgggctc
     7321 tcccgcgagc gaccaggcgc gcgccgcgtc ggccgcgatc gccgcgagcg gctcggcgcg
     7381 gaacgcgcgc tcgaaccaga gcgccgcgtc gcgatgccgc ccggcgctgg ccaggaagac
     7441 ggcgaggcgc gccgccggct cggggtcgta cggcgcgagg cggcaggcgg cgagcagctc
     7501 gccgagggcg cgatcgggct cgccggccgc cgccagcgcc gtggccgcgt tcagccgcgc
     7561 tccgggaaag tcgtagccca cgcgcgccgc gtccaggaac caggggagcg cctcggccac
     7621 ctgcccgcgc gcccagagcc ggcgcgccgc ggcgtccgag taggagaccg ccatcttccg
     7681 ggtcacgtag tcgtagcgct ccgggtggcc ttcgaggaga tcgctcgagc ggcgccatcc
     7741 ggcgggcggg tcccagccgg ccgcgtccga acccgacggc gcggccgcgt acaagagccc
     7801 gcgctgccgg aacgaaacgc cggcccggat gaggtcggcc ggaggaacgg cgtagcagag
     7861 ggtctccgtc ccgcgacgga tccgctccat ctcgtcctga tgcgccaggg ccgcccattc
     7921 ggcgcgcggc ttcgagcgga gcccgcacgg atcgccgaag atccatccgc gccggtggcg
     7981 gagggtcacg tccggacggc acccctccac gcgcgaggcg taggcggcga ggaacgtctc
     8041 gttgtcgccg tccagcacca gcgtggcccc gcggggcgct tcggcgagaa ggtctttgcc
     8101 gtagcgctcc gcgagcgtga agcggcttcg gtcggccgcg gcatagcgcc ccgggacgag
     8161 ggcgagcgcg gccgccacca gcgccgcgga ggcgagcgcg cggcgaaccc cggcactccc
     8221 gagggagccg ccgatgcccg cggcgcgccg ggcgcgaagg cgggcggcga atccggcgac
     8281 cgactgggcg ccggcccccg cgaagagcgc tcccgccgcg acgaggggcg ccaggaaggg
     8341 cgtcacctgc gccaggtgct cggcgtccgg cgtaaaggcg atgagcgcga tcagcgccgc
     8401 gggaatggtc gccgccgcca gcgcaagcgc acccgcgagg ctccgcgcgc gacgcgcgaa
     8461 gagccccgcc gccgcgaggg ccgcgcccag ccatccgagc gacgcgatcg cgattccacc
     8521 catacccgtg acttcgtcga aggcgcggtc caggcggaag gggttctgtg ccagtccgcc
     8581 gtacgtgcgg cggaggagga agtcggccag tcccgaaaga ccgcgcacgt cggtccaggc
     8641 gaaggaggga cccgcgctcc agcgcaccgg aatcacgagc gtgagggaga agccgagcac
     8701 ggcccacccc atcccgcgcg cgatgcgcgg cgcgcccgcg ccccgtcgc
//
LOCUS       DASSLZ010000088        25992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_918912, whole genome shotgun sequence.
ACCESSION   DASSLZ010000088 DASSLZ010000000
VERSION     DASSLZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 25992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25992
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918912"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFM00_03215"
     CDS             <1..340
                     /locus_tag="VFM00_03215"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HET7497286.1"
                     /translation="EALLSGLFNPRRRATAEVIRRAQASGALGKRVAPLVAVDLLFGP
                     LFYRKFVRQEPMTKAFVRQVFDGVLEGLGPRPGRRSASPRSRPPVRPLHSRTTIRTTE
                     VPKRVRARAK"
     gene            complement(354..854)
                     /locus_tag="VFM00_03220"
     CDS             complement(354..854)
                     /locus_tag="VFM00_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HET7497287.1"
                     /translation="MTEGRVVKKVGDDVSTDIIYPGRFMATVLPSETPQLAFADDAAF
                     NVALKSGGVPPGSVLVAGKNFGCGSSREQAASCLRGHDLVVVARDFARIFLQNGINLG
                     LRMVICPTIEAEEGDILGFRDGAVVNRRTGRSYPVVPLPKARQAIIDAGGLVPYTRRL
                     LLAKPA"
     gene            complement(856..2145)
                     /locus_tag="VFM00_03225"
     CDS             complement(856..2145)
                     /locus_tag="VFM00_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET7497288.1"
                     /translation="MGMTYVQKLLARTLGRRDLAVGDVVEPAVDLAMSHENAALVLNQ
                     FHEIFQGTGREAKVWDPQRIAIVFDHRVPAESAKTASNQKKVREFVARAGITKFHDVR
                     GDVGGICHQILPENGYVRPGAVVVGTDSHTTTHGALGAFAFGIGATEMASVWTLGRIL
                     NVEVPGTIQVVVNGTFRDDVYPKDLILHLIGEISAEGANYRVLEFTGSAIHAMSTSGR
                     LVLCNMAVEAGATAGVVPPDAETLRYLKDEAGVKERIEIFGPDPDAAYERVIHIDAGA
                     LTPQVACPHAVDNVKPVTSVKGTKVQQIVIGSCTNGRLDDIEVAARTLKGRKIAAGTR
                     MLVFPASWRIFHQALDRGYLRDLMEAGAVVCNPGCGPCLGVHQGALGDGEVALSTTNR
                     NFKGRMGNPTGEVYLCSPAVAAASAVAGVITDPREAH"
     gene            complement(2150..3376)
                     /locus_tag="VFM00_03230"
     CDS             complement(2150..3376)
                     /locus_tag="VFM00_03230"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7497289.1"
                     /translation="MIGTAGSNGTRRPMLEGIRVLDLTNVLAGPFATLHLALLGADVV
                     KIENPKDGDLARKLGIVPELNEQLMGTSFLAQNANKKSVTLNLKTPEGKEIFLRLAKE
                     ADVLVENYRPDVMERMGLGYATLAAQNPRLIYCAISGFGHTGPEALKPAYDQIIQGLS
                     GVMAVNGDERLNPLRAGFPVCDTVGGLNAAFAILAALYHRERTGEGQFVDVAMLDSIM
                     PLMGWVAANLLIGGQQPVLMGNDNFTAAPSGTFRTRDGWVNIAANKQEQWEALCEELG
                     LTELLADPRFQKRDTRKKNRKELTPILEARLTQCPTSVWVQRLNARDVPTGAILSLED
                     ALRQPQIRHRGTLADVPVEGIGTIPLFGLTAKFEKTPGAITGPPPRLSAHTAEVLAGA
                     GVGADQLGALRAKGVV"
     gene            complement(3373..4293)
                     /locus_tag="VFM00_03235"
     CDS             complement(3373..4293)
                     /locus_tag="VFM00_03235"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HET7497290.1"
                     /translation="MNRIVLHEVGPRDGLQMEKQIVSTAKKESWIRKIMASGVDAVQV
                     GSFVHPDKVPQMADTDKLFRRLVPQPGAKSGGPVLSGLVLNEKGLERGLTCGVELFCM
                     GASASETHSLANTGMGSEEATTRIIATARKAVAAGHRVQLSVQSAFGCGFEGPVPEER
                     VLEMVRAYVDAGFRSISLADTAGHARPSQVERLFGAVFGIDPTIECACHFHNTYGLGM
                     ANLFAAMRAGVTSFETSFAGLGGCPFTKVAAGNVATEDFVHALHLEGLRPDVDLDRVI
                     EVAREVAAHFGRDLPGFVYKTGPIPTKVPA"
     gene            complement(4330..5493)
                     /locus_tag="VFM00_03240"
     CDS             complement(4330..5493)
                     /locus_tag="VFM00_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HET7497291.1"
                     /translation="MAKYTIAWLPGDGIGVEVMEAARLVLDAVQFDATYTNGDIGWEF
                     WRREGDPFPQRTIELLRGVDAAMFGAITSKPLREAECELEPPRKGKGLVYRSPIVRMR
                     QLFDLYVCLRPCRAFPGNPLNYKEGIDLVVFRENTEDLYAGVEFPTVPDRLRDTLTAL
                     SPAFKPFSEMAGDEYAISCKVNTKHGSERIVRAAFEFARKFGRKKVTVVHKANVVRAT
                     DGLFLEEARRVAKEFPEIAMDDANIDAMTMWLLKNPFNYDVIVAPNLYGDIISDLCAQ
                     MVGGLGFGCSGNIGSDVAVFEPTHGSAPKHAGKYKANPIATILAAKMMLDWLGETERA
                     RAIENAVAEVIREGTVRTYDMGGAAGTLDMARAIEAKCRAAFPASHRAGTTPA"
     gene            complement(5607..6740)
                     /locus_tag="VFM00_03245"
     CDS             complement(5607..6740)
                     /locus_tag="VFM00_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7497292.1"
                     /translation="MTLPFNVSRTLAVAERDLRRFRRNIQLVIPMVMMPIIYLLILGK
                     SMGGDLHHIPVAIVVEDEGAAAAAVRKQMMTLQAARQLFLVREESDVRAAVDRLRQGD
                     YRAVVIVPPNFSEDFARGEKAELGLALDNTDGTSSNTIEGEMRRAIADLRVEGRAPPM
                     ARVGVTVERVDVYGHKNYMQYLVPGVIALALFFVAMVAGGIILVDDRARGIHEGYFVT
                     PLTSLDLVMGLTLSATTLALCMGTLVTVSSIAIAQLPLIGGLPTLLMVEFTIFLLGLG
                     LVLFMFTLMARVSNPMTPRALFGILNVITFFPSGALYPTESYPGWLQALSRVNPMRYA
                     VHALRNLLLKGVGFQAVLPDFLILIAFAGVMLVLASTLFKRTL"
     gene            complement(6744..7763)
                     /locus_tag="VFM00_03250"
     CDS             complement(6744..7763)
                     /locus_tag="VFM00_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7497293.1"
                     /translation="MIPDEAADPAAPAIVVEHLTKKFGDFTADDDLNFTVARNEIFGI
                     LGPNGAGKSTLVRMLTTLLRPTSGRALVMGFDVARRTNAVRHSIGVIPQANTVDSDLS
                     AWESLDIYGKFYGMSRKERIDRSTELLQAVGLWDWKDQPAGTYSGGMRRRLEIARGLV
                     HRPHVFFLDEPTTGLDPQSRRVIWELLEKLRGESELTVLICTHYMDEADRLCDRLAII
                     DHGKIAALGTPKELKESVPGQEAVRVEFFREVAEPLRESLRSLPDAKDAAREGDHAVR
                     IVLTTDVVPLEAILEAARRADNKVKSVTLEEPTLDDVFIHYTGRGLRDAVSTEYVRPL
                     PSIMR"
     gene            complement(7760..8704)
                     /locus_tag="VFM00_03255"
     CDS             complement(7760..8704)
                     /locus_tag="VFM00_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7497294.1"
                     /translation="MAEKAAPSRAKRIRGLILLLVIVGGGIAIWKYQHRREGYTGGDV
                     VTTGTVEAVQVSLGFKVPGKIADVPVNEGDRVQPGQVIAQLDAQDLDVAVRSAQAALE
                     SARAALGQARANREKAARDLARMRELSREGASTQQQLDAAVAAAAVGRAQVDAAAAQV
                     HQAESALAQAQLTRSYAVVRSPLAGQVTEKIHHPGEMVTVGTAVVTIADLDTLKVHAA
                     VDETRVGAVRPGDSVSVRVYTFDKKVFAGVVTDVSPSGDFATRKDWGAQRRDIRTFDV
                     TARIPNPEGLLKDGMTADVTIHAGTPGAVPEARAEGAR"
     gene            complement(8709..10055)
                     /locus_tag="VFM00_03260"
     CDS             complement(8709..10055)
                     /locus_tag="VFM00_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7497295.1"
                     /translation="MRRARLLQVALAGAAVLAGAPAARALTLEQCIELARRNAPALRV
                     SEAGVSRAGQAIREARAALSPTLRVAGSAIERSESPKFIISVPGSPSPAVVKTGSATN
                     VDVRAEARLPIDASGQSRALVRAAEAARKSQQRAAEEADADLVLRVSQAYYRVIAAGR
                     LETAAGEAVQAAGARRRLAASQVRAGVAQRLDSLQARVDLFQRESALIRAHEAVQTSR
                     VELETAIGTPIDSTETLEPPGSPDPAIPEAAALEATALRSRAQLAAFDEQLRENEQRI
                     RAARAARGPTMGLSGTAQYLGPNRQEEFFNTQDPGLKTYNLWAGVDFSYSLFDGGLSS
                     ARAGELRADRAALEARRRQAELEVRRDVERSLSDLRVSLAIWQSDSSRVTAAREALRL
                     AEAGYRGGTTTASQVRDAESALADARAQEAQSLLDTWSARAALLRAAGPATSTGGR"
     gene            complement(10052..10675)
                     /locus_tag="VFM00_03265"
     CDS             complement(10052..10675)
                     /locus_tag="VFM00_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7497296.1"
                     /translation="MSAPEFFPKLRLKSGDRRSQILAAALDVFAELGFHGARTRDLAE
                     KAGVSEALLFRHFATKEDLVRAILESVGFEERIGMMEEHFTKLPPREGLCRIAEYSLT
                     ALRDDPRVFRVIFFGVLEVPDLAGEFYRKFLSRLLALETRLFERAFVERGARRAAQDA
                     AVAARSFHGSLLFYQMTGAIVRIEPLPQNPKERAEAIVNLYLPEEAA"
     gene            10883..11308
                     /locus_tag="VFM00_03270"
     CDS             10883..11308
                     /locus_tag="VFM00_03270"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7497297.1"
                     /translation="MIRKASAEWRGGLKDGKGTLSTDSGVLTNAQYSFSTRFEDGKGT
                     NPEELVAAAHAGCFSMALSAQLGAAGITPESVRTAAAVTLEKVGDGFSVTASHLDVTA
                     RIPGGDRAAFQKAAEQAKAGCPISKLLNAKITMDAKLEA"
     gene            complement(11348..12319)
                     /locus_tag="VFM00_03275"
     CDS             complement(11348..12319)
                     /locus_tag="VFM00_03275"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HET7497298.1"
                     /translation="MWKFFGRGIRARNRRELFLSFYAQISLILVFAVWATGIIVGFSV
                     LHWSLGSTVHGSSGMTGYPADLYLSGSTFFTLGLGDVVPMTGLSRFLTVLEAGLGFGF
                     LALVIAYVPVLYQSFSPREARITMLDEWAGSPPTAGQILRRAFESQNPGEELNRLFRD
                     WEAGAANILESHLSYPILAFFRSQHDNQSWLGSLCSVLDAAALVSAGVKGVDPFQARL
                     TFAIGRHTLVDVSQVFRLTPLRDGFERIGPEQIAELRRWLVAAGVPLDESAEAGARLE
                     ELRGLYAPYVAALSEYLAMPLPALIPAEKSRFNWKTTAVGPIHDGAH"
     gene            complement(12454..13506)
                     /locus_tag="VFM00_03280"
     CDS             complement(12454..13506)
                     /locus_tag="VFM00_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7497299.1"
                     /translation="MGPRRFVSDLSEAMREGLSQGALDEAALDRLLHSPDFDAGAFDL
                     FLAEARRQGVTLPEGAASPEVSVPDEAGHGIGDLERRYLKEIQRYPILQRDEERALWD
                     SMRLGVEDARKRLILSYLRLVVSQARAYRNRGVEFLDLVEEGNLGLITAVDRYDVDRG
                     IHFATYATWWIRQALARGVANQSRTVRIPIHVLQMMRRFIATQRRLETERRRPPDLEE
                     IARVMGIPMTRAKRLETLVHSVRSLDVDLSNEAFHGLVESEAVEQPPTLDEIVELQLR
                     DQQVNEVLKQLSEREEAILRFRYGFFDDRPRTLAETGAQFGLSRERIRQLEQRALIKL
                     RTLLESESTESQPSVH"
     gene            complement(13518..13832)
                     /locus_tag="VFM00_03285"
     CDS             complement(13518..13832)
                     /locus_tag="VFM00_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET7497300.1"
                     /translation="MTAPREAGASRGDGGRVRFVAVVAGRVQGVGYRFFAQERARARG
                     LAGSVRNLPTGGVEVDAEGPDEILKAYLLDLHQGPPAARVRDVTVRWLSAQGARDFVI
                     RT"
     gene            complement(13854..13927)
                     /locus_tag="VFM00_03290"
     tRNA            complement(13854..13927)
                     /locus_tag="VFM00_03290"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13891..13893),aa:Pro,seq:cgg)
     gene            complement(14016..14678)
                     /locus_tag="VFM00_03295"
     CDS             complement(14016..14678)
                     /locus_tag="VFM00_03295"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET7497301.1"
                     /translation="MNPEDQGAARRRMVQSQLRERGIHDPHVLRIMEALPRHLFVDEA
                     LEAKAYSDSALPIGAAQTISQPYMVALMTQALELNGEEKVLEIGTGSGYQTAILAELA
                     DRVFTVERIASVGVAARDRLTKMGYTNIVFRVADGSVGWREMAPFDRVLIAAGAPRTP
                     HFLDEQLKPGGIVVMPIGVGENQMLVKAVRTEAGWQRRELCGCAFVPLIGRDAWERRE
                     SR"
     gene            complement(14680..15432)
                     /gene="surE"
                     /locus_tag="VFM00_03300"
     CDS             complement(14680..15432)
                     /gene="surE"
                     /locus_tag="VFM00_03300"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET7497302.1"
                     /translation="MILVTNDDGIAAEGLRTLAEAMRPLGEVTIIAPDREQSATSHSL
                     TLHRPLRLRKTGPGILSVDGTPTDCVLLAVHGFLKEAPKLIVSGINHGPNMGNDVLYS
                     GTVSAASEGSFLGIPSIAFSLATWEPTDFAPAGRIATALVRMLLQRGLTPGMCLNVNI
                     PPLPYAEIRGLRVARLGRRVFHDVIVEKTDPRGKSYYWIGGENPTWDPDEASDFHAVS
                     NGYVSMTPLSFDVNDYKAIVELETMGLTIHDE"
     gene            complement(15429..16091)
                     /locus_tag="VFM00_03305"
     CDS             complement(15429..16091)
                     /locus_tag="VFM00_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7497303.1"
                     /translation="MRVCAVIPARDAADTVGPVVRGLKATLPEATILVVDDGSSDETG
                     ARAKEAGAEVIRHTLNQGKGAALQTGFDEAVRRGADAVLALDADGQHDPAWAPRLLAG
                     LESADLVVGSRLQSREGMPWLRRVTNDVTSWWVSRLARTLIEDSQSGYRAIRAPVLRR
                     VRPEARRFEYESEFLVAAARAGFRIAAVGIPTLYNAPGSHIRPVRDTVRFVRFVLRHL
                     FR"
     gene            complement(16091..17002)
                     /locus_tag="VFM00_03310"
     CDS             complement(16091..17002)
                     /locus_tag="VFM00_03310"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497304.1"
                     /translation="MTDLGYHIASIAARRLPVWMTDRMAAWIADVYVSTHPSRAREAA
                     HRVGRAWHAGGLGGPPPPARETFRAFAWALRDFLAARGGGFRVPAVRLDAEAARHLAA
                     ARAAKAPTLVVSGHFGPWEMALWWLAREVGPMEALSAPHRLEAVERFFRARRAAFGVR
                     TLGGERPAAEALRRLRAGGWIAALADRSWRPAAPETIPVGERLVAVDAAPLRLAQRAG
                     AQVLPGAAWRDADGQIAVRFHAPFTLDPERGGLTVDEALASLSRFFDGHVRAHPTQWF
                     DWSIRSARPAPRGRAAQERPLARSRAR"
     gene            complement(17106..17182)
                     /locus_tag="VFM00_03315"
     tRNA            complement(17106..17182)
                     /locus_tag="VFM00_03315"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17146..17148),aa:Pro,seq:ggg)
     gene            complement(17308..17691)
                     /locus_tag="VFM00_03320"
     CDS             complement(17308..17691)
                     /locus_tag="VFM00_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7497305.1"
                     /translation="MKSLPTGKLYYSISEVSDLVGVKPHVLRYWETQFKMLRPRKGRG
                     GARMYRKRDIEVLFDIKQLLYDQRFTIAGARRKILDDKQDGKDQIELPFTKFDKEETI
                     RSLKKDMESLLHMLKQDTAKRARGR"
     gene            complement(17696..18718)
                     /locus_tag="VFM00_03325"
     CDS             complement(17696..18718)
                     /locus_tag="VFM00_03325"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET7497306.1"
                     /translation="MNIAVIGGGGWGTALAALLTKNGHTVRLWVYEADLAERMGRERR
                     NDVFLPGVPIPEAVAITADLAEAAADSSAILFATPSHALRATASRLAAEKTALAARLV
                     VIATKGLEKDTHRRMSEVVQETLAAPLGTDPVVLAGPSHAEEVARGIPTAVVAACRDH
                     GRAAAVQEAFSSETFRVYTNDDVLGVEIAVALKNVVAIAAGISDGLGFGDSTKGALLT
                     RGLAEMARLGEALGARPETFSGLAGMGDLIATATSRHSRNRRLGEAVGRGATLAEALA
                     ASPMVAEGVGTAQAAVDLAARLGIELPIAEQVRAILYEGKSARSAMRELLTRDLKPEA
                     RTVSRR"
     gene            complement(18715..19326)
                     /gene="plsY"
                     /locus_tag="VFM00_03330"
     CDS             complement(18715..19326)
                     /gene="plsY"
                     /locus_tag="VFM00_03330"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET7497307.1"
                     /translation="MAIPFHPIPVILGFLLGSIPSGLWIARARGVDLRTVGSGNIGAT
                     NAIRGLGPRWGGLVFAIDTAKGAVAALLGGGHVPTALAAGAAAILGHVFSPWARFKGG
                     RGVATSLGVFLAILPIPALLALAVWIVLFALSRRVSVGSLGAAIAYPLLVLWLAPHDE
                     YRLARVVAGILVALLVVIRHIPNIRRLAAGTEAPSFGGKGAKT"
     gene            complement(19354..20667)
                     /gene="der"
                     /locus_tag="VFM00_03335"
     CDS             complement(19354..20667)
                     /gene="der"
                     /locus_tag="VFM00_03335"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET7497308.1"
                     /translation="MLPIVAIVGRPNVGKSTLFNRILGQRRAIVDELSGLTRDRHYAE
                     GEWSGRKFLLVDTGGIDPASAHPIQKQILQQTQVALDEADVAMVVADAQSGVTPLDIE
                     VAERVRRRGRPTLLVVNKADQPSREEVVAEFFSLGLGEPMPVSALHGLRSGDLLDEVV
                     RLLPEREAETEEPDTIRIAVLGRPNVGKSSLVNRLLGQERMVVDSVAGTTRDAVDTTL
                     QAHGRSFRLVDTAGLRRETKVNDPVEFYSVTRALRAVSRADIVLLVIDVGREPARQDA
                     RLAALAEEQRKGLVVVFNKWDLVEEPAAAKAEIEEEFTKVYPFLEHVPRLYVSALSGR
                     GVKKILPTAVTVFEEYARSIPTSTLNEAIGAILNRVSPPATAEGRHLKFYYAAQIGTR
                     PPSFALFVNNPRYRAKNYVSYLESGLRQRFGFQGTPIILEWRRSE"
     gene            complement(20673..21968)
                     /locus_tag="VFM00_03340"
     CDS             complement(20673..21968)
                     /locus_tag="VFM00_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HET7497309.1"
                     /translation="MIGLSDPFTIAKVEPGSLGERLGLLPRERILEINGVPLHDEIDF
                     GSQISEEHLRLLVRSESGAEREVEGFREYGVPFGIEFEARQPKRCHNNCVFCFVYQHP
                     KGVRRELLIKDDDYVFSFVHGNFITLTNLGEAEFQRILDERLSPLYVSVHATDPAVRV
                     RMMKNPKSGKILADIDRLAAGGIDLHTQLVVVPGMNDGAVLTQSIEELAARYPRVKTI
                     AVVPVGLTKHRGRLPDLRTFTREDAVFALDEVHAFQASLLKRHKTRIVFAADEMYVLA
                     DQAVPPARAYEGFPQLENGIGMLRQTIDAWSKGTDAIRPRNGTRERVAIVTGTSAAPT
                     LRRLLDERPPQGVDASLCVVTNEYFGDTVTVSGLLVGADIQAALLRHGPVDRVLLPPN
                     CLKEREIFLDDRTRTDLESAVGAPVQIGFDESGRSERAA"
     gene            complement(22036..22110)
                     /locus_tag="VFM00_03345"
     tRNA            complement(22036..22110)
                     /locus_tag="VFM00_03345"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22076..22078),aa:Gly,seq:ccc)
     gene            complement(22135..23112)
                     /gene="miaA"
                     /locus_tag="VFM00_03350"
     CDS             complement(22135..23112)
                     /gene="miaA"
                     /locus_tag="VFM00_03350"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET7497310.1"
                     /translation="MPAVPDSVHPENPAQRRVVLLGPTAGGKSEIALHLAEAIGAEIV
                     GADSRQIYRGLEVGTAAPGAADRARAPHHMASFLSPAETYSAGRYAREARAAVSEVEG
                     RGATAVIVGGSGLYLRALLDGLFAGPERNEQVRAELMARLEAEGLEALRAELLRVDPA
                     AHAKIFPGDSVRVIRALEVYRLTGTPISALRRERRNEPLPARRFGIRWSRQALAQRIE
                     DRIRQQLRDGFLEEARALKEAGLPPDAPGLHTLGYRELLEYLRGRVTLEEAVQTVALR
                     TRQLAKRQETWFRRTAGVRWFDLESPAEFPAVALQIRREMLLDQGEETP"
     gene            complement(23114..24991)
                     /gene="mutL"
                     /locus_tag="VFM00_03355"
     CDS             complement(23114..24991)
                     /gene="mutL"
                     /locus_tag="VFM00_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET7497311.1"
                     /translation="MASIRVLPPEVASKIRAGEVVDRPAAVAKELIENAIDAGSRLIA
                     VQVAASPDKLIRVQDDGAGMSREDALLSVRRHATSKLSSEEDLEAIRTLGFRGEALAS
                     IAEVARVSLSTRSASELTGTQVEVLGGTVIGVSGVGRAVGTTVSVEDLFFNTPARLRF
                     LKSRESEIRAVARAAWGYILTYPAIHWRFTSAGREDVDLPAAEDLLERWQVLYGRGAS
                     EGASAFAQESGEIIVRGVLGTPEQARAGREQQILTVNGRLVVSPALGTALRQGYGNLI
                     PGDRHPMAVLMIEIDPTQVDVNVHPTKREVRFRDEGRVFQAVKQAVDATMRRHVPVSL
                     DIPEVRLSRPAPAEPSTPGVTPAQGAGVPGSAGPDAQVDEGAAYWGAQGALLPRPRAS
                     ESATALGAAPAATQTLIEEYAPAPRLRPERVEEGGQAAVRENLLETEIPIWQLHDRYL
                     LAPIRGGLVVVDQHAAHERILYEQARARLYGEEADSQHLLFPRVLDVTAEELDHLLLL
                     EVQIRRLGYEVSLFGEKQVAIRAVPASIPEEAAVEALRSLLAGEDAHGGGDEPPEERV
                     AKSFACHAAVRSGQPLRPEERRALIDRLFATDLPHGDPHGRATYVRVSMEELDRRFGR
                     R"
     gene            complement(24991..>25992)
                     /locus_tag="VFM00_03360"
     CDS             complement(24991..>25992)
                     /locus_tag="VFM00_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET7497312.1"
                     /translation="LVATFDAPDVQKAGIFDEVRTYKEGPKAALLEILDGAKPRSIGI
                     NVSEDDALADGLTAGLRDYLGNTLSGTPHARRLTHAGKFIAHLRSVKLEDERLRIERA
                     IQQTEGIFDRVRDALAIGMTAREVAALAHAEADRLGAPTAWPRRHCPTVTVGPHAAVG
                     HVAPGEEKVIPGCLVHMDFGIVLDGYCSDLQRLWYVAAGNGDAPAEVRRAYAAVVEAI
                     DRSAKALRPGAAGWEVDQVARGHLTGSGYPEYAHALGHHLGRAVHDGGGVLGPRWERY
                     GKEPFEPVRVGNVYTLEPSLWVDGHGLVSLEEDVVVGPDGARFLSRFPRDLPILRRS"
BASE COUNT         3832 a         9214 c         9100 g         3846 t
ORIGIN      
        1 cgaggccctg ttgagcggcc tcttcaaccc gcgacggcgc gccaccgccg aggtcatccg
       61 cagggcgcag gcatcgggag ccctcgggaa acgggtcgct cccctggtcg ccgtggatct
      121 cctcttcggg ccgctgttct atcggaagtt cgtgaggcag gagccgatga cgaaggcctt
      181 cgtgcggcag gtgttcgacg gcgttctgga ggggctgggc ccgcggccgg gtcggcggag
      241 cgcctcgccg cggtcacgtc cccccgtgcg cccccttcac tccagaacga cgatccgcac
      301 cacggaagtg ccgaagcgcg tgcgggcccg cgcgaagtag cgtcccgatc ctcctacgcc
      361 ggtttcgcga gcaggagcct ccgcgtgtac gggaccaggc cccccgcgtc gatgatcgcc
      421 tgccgcgctt tcggcagcgg cacgaccgga tacgagcggc cggttcgccg gttcacgacc
      481 gcaccgtcgc ggaagcccag gatgtccccc tcctcggcct cgatcgtcgg gcagatcacc
      541 atgcggagcc cgaggttgat gccgttctgg aggaaaatgc gcgcgaagtc ccgcgccacg
      601 accacgagat cgtgccccct gaggcacgac gcggcctgct cgcgggagga gccgcacccg
      661 aagttcttcc cggccacgag gacgctgccc ggaggcacgc cgccggactt cagcgcgacg
      721 ttgaaggccg cgtcgtcggc aaaggcgagc tgcggcgtct cgctggggag caccgtggcc
      781 atgaaccgcc ccggatagat gatgtcggtg gagacgtcgt ctcccacttt cttcacgacc
      841 cgtccttccg tcatgtcagt gcgcctccct cgggtcggtg atcacgccgg cgaccgcgct
      901 cgcggcggcg acggcgggcg agcagagata gacctcgccg gtcgggttgc ccatccgccc
      961 cttgaaattc cggttcgtgg tggagagggc gacctcgccg tcccccagcg ccccctggtg
     1021 cacgccaaga caggggccgc agccgggatt gcacaccacc gctcccgcct ccatgagatc
     1081 gcgcaggtag ccgcggtcca gcgcctggtg gaagatgcgc cacgacgccg ggaagaccag
     1141 catgcgcgtc ccggcggcga tcttccgtcc cttgagcgtg cgcgccgcga cctcgatgtc
     1201 gtccagcctg ccgttggtac acgagccgat gacgatctgc tgcaccttgg tccccttcac
     1261 cgacgtgacc ggcttgacgt tgtccacggc gtgcgggcag gcgacctgcg gcgtgagcgc
     1321 tcccgcgtcg atgtggatca cccgctcgta ggcggcgtcg ggatccggac cgaagatctc
     1381 gatccgctcc ttcacgccgg cttcgtcctt cagatagcgc agcgtctcgg cgtcgggcgg
     1441 cacgacgccg gcggtggcgc ccgcctcgac cgccatgttg cagagcacga gccgtcccga
     1501 cgtggacatc gcgtggatcg ccgagccggt gaattcgagg accctgtagt tcgccccctc
     1561 cgccgagatc tccccgatca ggtgcaggat caggtctttc ggatagacgt cgtcgcggaa
     1621 ggtgccgttc acgacgacct ggatggtgcc gggcacctcg acgttcagga tgcgtcccag
     1681 cgtccagacc gaggccatct cggtcgcgcc gatgccgaag gcgaaggcgc ccagcgcgcc
     1741 gtgcgtcgtg gtgtggctgt cggtgcccac gacgaccgca ccgggcctga cgtagccgtt
     1801 ctcgggcagg atctgatggc agatcccccc gacgtcgccc cgcacgtcgt ggaacttggt
     1861 gattccggca cgcgccacga actcgcgcac cttcttctga ttgctcgcgg tcttcgccga
     1921 ttcggcgggc acgcggtggt cgaagacgat cgcgatccgc tggggatccc agaccttggc
     1981 ttcgcggccg gtcccctgga agatctcgtg gaactggttc aggacgaggg ctgcgttctc
     2041 gtgggacatc gcgagatcca ccgccggctc gaccacgtcc ccgacggcca gatcgcggcg
     2101 gccgagggtg cgcgcgagga gcttctgcac gtacgtcatg cccatggcgc tacacgactc
     2161 ctttcgcccg gagggctccc agctggtcgg cgcccacgcc ggctccggcg agcacttcgg
     2221 cggtgtgcgc cgagaggcgc ggcggcggcc cggtgatggc gcccggtgtc ttttcgaact
     2281 tcgcggtgag gccgaagagc gggatggtac cgattccctc caccggcacg tcggccaggg
     2341 tgcctcgatg gcggatctgc ggctggcgca gggcatcctc gaggctcagg atggcgccgg
     2401 tcgggacgtc gcgcgcattc aagcgctgga cccagaccga ggtcggacac tgcgtgagac
     2461 gcgcttcgag gatcggcgtg agctccttcc gattcttctt tcgcgtgtcc cgcttctgga
     2521 agcgcgggtc ggcgaggagc tcggtcaggc ccagctcctc gcacagcgcc tcccactgct
     2581 cctgcttgtt cgcggcgatg ttcacccatc cgtcccgggt ccggaacgtc cccgacggcg
     2641 ccgcggtgaa gttgtcgttc cccatcaaga ccggctgctg cccgccgatc aggaggttcg
     2701 ccgcgaccca gcccatgagc ggcatgatcg aatcgagcat cgctacgtcg acgaactgcc
     2761 cctcgcccgt ccgctcgcgg tggtacaggg cggccagaat cgcgaacgcc gcgttcagcc
     2821 cgccgacggt gtcgcacacc ggaaagccgg cgcggagcgg gttcaaccgc tcgtcgccgt
     2881 tcaccgccat gacgccgctc agcccctgga tgatctggtc gtaggccggc ttcaaggcct
     2941 cggggccggt gtgcccgaac cccgagatgg cgcagtagat caggcgcggg ttctgcgcgg
     3001 cgagcgtcgc gtagcccagc cccatccgct ccatcacgtc ggggcggtag ttctcgacga
     3061 ggacgtcggc ctccttggcc aggcggagga agatctcctt cccctccggc gtcttgagat
     3121 tcagggtgac cgacttcttg ttggcgttct gggcgaggaa ggaagtgccc atgagctgct
     3181 cgttcagctc gggcacgatg ccgagcttcc gggccaggtc gccatccttt gggttctcga
     3241 tcttgacgac gtcggcgccg agcagcgcca ggtgcagcgt cgcgaaggga ccggcgagca
     3301 cgttggtcag gtcgaggacg cggatcccct ccagcatcgg gcgcctggtt ccgttgctcc
     3361 ccgccgttcc gatcacgcgg gcaccttggt cgggatcggc cccgtcttgt agacgaaccc
     3421 gggcaggtcg cgcccgaagt gtgccgcgac ctcgcgcgcc acctcgatca cgcggtccag
     3481 gtcgacgtcc gggcgcagcc cctccaagtg cagcgcgtgc acgaagtcct cggtcgcgac
     3541 gttgccggcg gcgaccttcg tgaaggggca gccgcccagc ccggcgaagg aggtttcgaa
     3601 ggacgtgacg ccggcgcgca tcgcggcgaa gagattcgcc atgcccaggc cgtaggtgtt
     3661 gtggaagtgg caggcgcatt cgatcgtggg atcgatcccg aacaccgcgc cgaagagccg
     3721 ctccacctgg ctgggccgcg cgtgccccgc cgtgtcggcg aggctgatcg agcggaagcc
     3781 cgcgtccacg taggcgcgca ccatctcgag gacgcgctcc tccgggaccg gcccctcgaa
     3841 tccgcacccg aacgccgact gcaccgagag ctgcacgcgg tgccccgccg cgacggcttt
     3901 ccgcgcggtg gcgatgatcc gcgtcgtcgc ctcttcgctt cccatcccgg tgttggccag
     3961 gctgtgggtc tcgctggccg aggcgcccat gcagaagagc tccacgccgc acgtcagccc
     4021 ccgctccagc cccttctcgt tcagcaccag gccggagagc acggggccgc cggacttggc
     4081 acccggctgc ggcaccaggc ggcggaagag cttgtcggtg tcggccatct gcggaacctt
     4141 gtccgggtgg acgaaggagc ccacctgcac ggcgtccacg cccgaggcca tgatcttccg
     4201 gatccacgac tccttcttcg cggtcgagac gatctgcttt tccatctgga ggccgtcccg
     4261 gggtccgacc tcgtgcagga cgattcgatt catggcgcct ctcgggggca cgggcgcgcc
     4321 cgtgccgctc taggcggggg tagtccccgc gcggtgtgaa gccgggaagg cggctcggca
     4381 cttcgcctcg atggcgcgcg ccatatcgag cgtgccggcc gcccccccca tgtcgtacgt
     4441 gcgaacggtt ccttcgcgga tcacctcggc gacggcgttc tcgatcgcgc gggcgcgctc
     4501 ggtttctcca agccagtcca gcatcatctt cgccgcgagg atggtcgcga tcggattggc
     4561 cttgtacttg cccgcgtgct tgggcgccga gccgtgcgtc ggctcgaaga cggcgacgtc
     4621 gctcccgatg ttgcccgagc agccgaaccc cagccccccc accatctggg cgcacaggtc
     4681 ggagatgatg tcgccgtaca ggttgggcgc gacgatgacg tcgtagttga acgggttctt
     4741 gaggagccac atcgtcatgg cgtcgatgtt cgcgtcgtcc atggcgatct ccgggaactc
     4801 cttcgccacg cggcgggcct cttcgaggaa gagcccgtcc gtggcgcgca ccacgttcgc
     4861 cttgtgcacc acggtgacct tcttccggcc gaacttccgc gcgaactcga acgccgcccg
     4921 cacgatccgc tcggagccgt gcttggtgtt caccttgcac gagatggcgt actcgtcgcc
     4981 ggccatctcg gagaagggct tgaaggccgg cgagagggcc gtcagggtgt cgcggagccg
     5041 gtcgggcacg gtcgggaatt cgaccccggc gtagaggtcc tcggtgttct cgcggaacac
     5101 cacgaggtcg atcccctctt tgtagttgag cgggttgccc gggaacgcgc ggcaggggcg
     5161 caggcagacg tacaggtcga agagctggcg catccggacg atcggcgagc gatagacgag
     5221 ccctttcccc ttgcgcggag gctcgagctc gcactcggct tcccggagcg gcttggaggt
     5281 gatggcgccg aacatcgcgg cgtcgacgcc gcggagcagc tcgatcgtgc gctgcgggaa
     5341 gggatcccct tcgcggcgcc agaactccca cccgatgtcg ccattcgtgt atgtggcatc
     5401 gaattgaacg gcgtcgagga cgaggcgcgc cgcctccatg acctcgacgc cgatgccgtc
     5461 ccccggcagc caagcgatcg tgtacttcgc catgtgcggg ccctcctccg gcccacggcc
     5521 atggtacggc ccgggacggg gcggcgcgct gctaccgagg gatcatggcc agcgggccgg
     5581 agggaccatc cggcggggag ggcggtctac agggtccgct tgaacagcgt ggaggcgagc
     5641 accagcatca cgcccgcgaa ggcgatcagg atcaggaagt cggggagcac cgcctggaag
     5701 ccgaccccct tgaggagcag gttccgaagc gcgtgcacgg cgtagcgcat ggggttgacg
     5761 cgcgagagcg cctggagcca gccggggtag ctctccgtgg gatagagcgc cccgctcgga
     5821 aagaaggtga tcacgttgag gatgccgaac agcgcgcgcg gggtcatggg attggagacc
     5881 cgggccatca gggtgaacat gaagagcacc aggcccagcc ccaagaggaa gatcgtgaac
     5941 tccaccatga gaagggtggg tagtccgccg atgagcggga gctgcgcgat cgcgatcgag
     6001 gagaccgtca ccagggtgcc catgcagagg gccagggtgg tggccgacag cgtgagcccc
     6061 atcaccaggt cgagcgaggt gagcggcgtg acgaagtacc cctcgtggat cccgcgcgcg
     6121 cggtcgtcca ccaggatgat cccgcccgcg accatcgcga cgaagaagag ggcgagcgcg
     6181 atcacaccgg gcaccaggta ctgcatgtag ttcttgtgcc cgtagacgtc cacgcgctcc
     6241 accgtcacgc cgacacgggc catgggagga gcgcgcccct ccacgcgcag gtcggcgatg
     6301 gcgcgccgca tctccccctc gatggtgttc gacgaggtcc cgtcggtgtt gtccagcgcc
     6361 aggcccagct cagccttctc gccgcgcgcg aagtcctcgc tgaagttggg cggcacgatc
     6421 acgacggcgc ggtagtcccc ctggcgcagc cggtccaccg cggcccgcac gtcgctctcc
     6481 tcgcggacca gaaagagctg ccgggcggcc tgcagcgtca tcatctgctt ccggaccgcc
     6541 gcggcggccg ccccctcgtc ctcgacgacg atcgccacgg gaatgtggtg caggtcgccc
     6601 cccatcgact tcccgaggat gagcaggtag atgatcggca tcatcaccat cgggatcacg
     6661 agctggatgt tgcgccggaa gcggcgcagg tcgcgctcgg ccacggcgag ggtgcggctg
     6721 acgttgaacg ggagggtcac gcgctacctc atgatcgacg gcagggggcg gacgtactcg
     6781 gtggaaacgg cgtcccgaag cccgcgcccc gtgtaatgga tgaagacgtc gtccaaggtc
     6841 ggctcctcca gcgtgacact cttcaccttg ttgtcggccc ggcgggccgc ctcgaggatc
     6901 gcctcgaggg gaacgacgtc cgtggtcagc acgatccgca cggcgtggtc cccctcgcgc
     6961 gccgcgtcct tggcgtcggg aagcgagcgc agggactccc gcagcggctc ggccacctcg
     7021 cggaagaact cgacccggac cgcttcctgt cccgggaccg attccttgag ctccttcggg
     7081 gtgccgagcg cggcgatctt gccgtggtcg atgatcgcga ggcggtcgca gagccggtcc
     7141 gcttcgtcca tgtaatgcgt gcagatcagc accgtcagct cgctctcgcc gcggagcttc
     7201 tcgagcagct cccagatgac gcgacggctc tgcgggtcga ggcccgtggt gggctcgtcg
     7261 aggaagaaga cgtggggccg gtggaccagc ccgcgcgcga tctcgaggcg gcggcgcatc
     7321 ccgcccgagt acgttccggc cggctggtcc ttccagtccc agagccccac ggcctgcagg
     7381 agctccgtgg agcgatcgat gcgctccttg cgcgacatgc cgtagaactt cccgtagatg
     7441 tcgaggctct cccaggcgga gaggtcggag tccacggtgt tggcctgcgg gatcacgccg
     7501 atcgaatggc ggacggcgtt cgtgcgccgc gccacgtcga accccatcac gagcgcccgc
     7561 cccgaggtcg gacgcaggag cgtcgtgagc atgcgcacga gggtgctctt ccccgcgccg
     7621 ttcggtccca ggatcccgaa gatctcgttc cgggcgacgg tgaagttgag atcgtcgtcg
     7681 gccgtgaaat cgccgaactt cttggtgagg tgctcgacga cgatcgccgg tgccgccggg
     7741 tccgcggctt cgtccgggat caccgcgccc cctccgcccg cgcctcggga acggcgccgg
     7801 gcgtgccggc gtggatcgtc acgtcggcgg tcatgccgtc cttgagcagc ccctccgggt
     7861 tgggaatccg cgcggtgacg tcgaacgtcc ggatatcccg ccgctgggcg ccccagtctt
     7921 tccgggtcgc gaaatcgccc gagggggaga cgtcggtcac gaccccggca aagaccttct
     7981 tgtcgaacgt gtagacgcgc accgagacgg aatcgcccgg gcgcaccgcg ccgacgcgcg
     8041 tctcgtccac ggcggcgtgc accttgagcg tgtcgagatc ggcgatcgtg accaccgccg
     8101 tcccgacggt gaccatctcg cccgggtgat ggatcttctc cgtgacctgc ccggccaggg
     8161 gcgagcggac cacggcgtag gagcgggtga gctgggcctg cgccagggca ctctccgcct
     8221 ggtgcacctg cgcggcggcg gcgtccacct gcgcgcgccc cacggcggcg gccgcgaccg
     8281 ccgcgtccag ctgctgctgg gtcgaagccc cctcccgcga gagctcgcgc atgcgggcga
     8341 ggtcgcgcgc ggccttctcg cggttcgccc gcgcctggcc cagcgccgcg cgcgccgact
     8401 ccagcgcggc ctgcgcgctc cgcaccgcca cgtccaggtc ctgcgcgtcg agctgcgcga
     8461 tcacctgccc cggctggacg cgatccccct cgttcaccgg cacgtccgcg atcttgcccg
     8521 ggaccttgaa gcccaggctc acctgcaccg cttccaccgt gcccgtggtc acgacgtccc
     8581 ctcccgtgta cccctcgcgg cggtgctggt acttccagat ggcgatgccg ccgcccacga
     8641 tgaccagcag caggatgagg ccgcggatcc gtttggcgcg cgaaggcgcc gctttctcgg
     8701 ccatgacgct accgtccccc cgtcgaagtg gccggtccgg cggcgcgcag gagcgccgcg
     8761 cgcgcggacc aggtgtccaa aagcgactgc gcctcctggg cgcgcgcgtc ggcgagcgcg
     8821 ctctcggcgt cgcgcacctg cgatgccgtc gtcgtcccgc cgcgatagcc cgcctcggcc
     8881 aggcgaagcg cctcgcgcgc cgcggtcacg cgcgaggaat cggactgcca gatggcgagc
     8941 gacacgcgga gatccgagag cgagcgctcc acgtcgcgcc gcacctccag ctcggcctgg
     9001 cggcggcgcg cttccagggc cgcgcgatcg gcgcgaagct cccccgcccg cgccgacgag
     9061 agccccccgt cgaagagcga gtaggagaaa tcgactccgg cccacaggtt gtaggtcttc
     9121 aggccggggt cctgcgtgtt gaagaattcc tcctggcggt tcggtcccag gtactgtgcc
     9181 gtgcccgaca ggcccatcgt cggcccccgc gccgcgcgcg ccgcccggat ccgctgctcg
     9241 ttctcgcgga gctgctcgtc gaacgcggcc agctgcgctc ggcttcgaag cgccgtggcc
     9301 tccagcgccg ccgcttcggg tatcgccgga tcgggcgagc cgggcggctc gagggtttcg
     9361 gtcgagtcga taggggttcc gatcgcggtc tccagctcca cgcgcgaggt ctgcaccgcc
     9421 tcgtgcgcgc ggatcagcgc cgactcgcgc tggaagaggt ccacgcgggc ctgcagcgaa
     9481 tcgaggcgct gcgccacgcc ggcgcgcacc tgcgaggccg cgagacgccg gcgcgctccc
     9541 gccgcctgca ccgcctcgcc ggccgcggtc tccagccgtc ccgcggcgat gacccggtag
     9601 taggcctgcg acacccggag cacgaggtcg gcgtccgcct cctcggcggc gcgctgctgg
     9661 cttttgcgcg cggcttcggc ggcgcgcacc agggcccggc tctgcccgct cgcgtcgatc
     9721 gggagccggg cctcggcgcg cacgtccacg ttggtcgcgc tccccgtctt cacgaccgcg
     9781 gggctgggcg atccggggac cgagatgatg aacttgggcg actcgctgcg ctcgatcgcg
     9841 cttcccgcca cccgcagcgt gggcgagagc gccgcgcgcg cctcgcggat cgcctggccg
     9901 gcgcgggaga cgcccgcctc cgaaacgcgg agcgccgggg cattcctgcg cgcgagctcg
     9961 atgcactgct cgagcgtgag cgcccgggcc gcgggcgccc cggccagaac ggccgcgccg
    10021 gccagtgcga cctgaaggag tcgggcgcgc ctcacgccgc ctcctcgggg agatagaggt
    10081 tcacgatcgc ctcggcccgc tccttggggt tttggggcag gggctcgatc cggacgatcg
    10141 cgccggtcat ctggtagaac aggagcgacc cgtggaagga gcgcgccgcg accgccgcgt
    10201 cctgcgcggc gcgcctggcc ccgcgctcca cgaaggcgcg ctcgaagagg cgcgtctcga
    10261 gggccaggag ccgggaaagg aacttccggt agaactctcc ggcgaggtcg ggcacctcga
    10321 gcaccccgaa gaagatcaca cggaagacgc gcgggtcgtc ccttagggcc gtcaggctgt
    10381 actccgcgat ccggcacaga ccctcgcgcg gaggcagctt ggtgaagtgc tcttccatca
    10441 tgccgatccg ttcttcgaat ccgaccgact cgaggatcgc ccggaccagg tcctccttcg
    10501 tcgcgaaatg acggaagagg agcgcctcgc tcacgccggc cttctcggcc agatcccggg
    10561 tgcgggcgcc gtgaaatccc agctcggcga agacgtcgag cgccgccgcc aggatctggg
    10621 accggcggtc gcccgatttc agtcgaagct tgggaaagaa ctccggtgcg ctcatggctc
    10681 ccctcgatgc cgcgccgtcc agcacgcggc gctaagtgcg tacttacttt ctacccagcc
    10741 gcgcctccga cgcaagcgaa aagtcacgag accgtcgggc ccctcgaatg cccgctacgc
    10801 ctgcgatggt tccggtttac cattgccccc ctgtcgtgga gtcacgcgat ggaccaacgc
    10861 ttccctttcg acaggaggac ccatgattcg caaggcatcg gcggaatggc gcggcgggct
    10921 caaggacggc aagggcacgt tgtccaccga cagcggcgtg ctcaccaacg cgcagtattc
    10981 cttcagcacg cgcttcgagg acggcaaggg gacgaacccc gaggagctgg tcgcggcggc
    11041 gcacgcgggg tgcttctcca tggcgctctc ggcgcagctg ggcgcggccg gcatcacgcc
    11101 cgagagcgtg cgcaccgcgg cggccgtgac gctggagaag gtgggcgacg gcttttccgt
    11161 gaccgcgagc cacctcgacg tgaccgcgcg catcccgggc ggcgatcgtg cggcgtttca
    11221 gaaagcggcc gaacaggcca aggcgggctg cccgatttcc aagctcctga acgccaaaat
    11281 cacgatggac gcgaagctgg aagcgtagcg ccgccggggc cgctccagcc gcccagagcg
    11341 cggcggctca gtgcgccccg tcgtggatcg gtccgaccgc ggtcgtcttc cagttgaatc
    11401 gcgacttctc cgcggggatc agggccggga gcggcatggc gagatactcg gacagcgccg
    11461 cgacgtaggg cgcatagagc ccccgcagct cctcgagccg cgccccggcc tccgcgctct
    11521 cgtccagcgg gaccccggcg gcgaccagcc agcggcgcag ctcggcgatt tgttccgggc
    11581 cgatccgctc gaagccgtcc cgcagcggcg tcaggcggaa tacctggctc acgtcgacca
    11641 gcgtgtgccg cccgatcgcg aaggtgagcc gggcctgaaa gggatcgacc cccttcacgc
    11701 cggcgctcac cagcgcggcc gcgtcgagca cggagcagag ggaccccagc cacgactggt
    11761 tgtcgtgctg ggatcggaag aacgcgagga tgggatagga gaggtggctc tcgaggatat
    11821 tcgcggcgcc cgcttcccag tcgcgaaaga gccggttcag ctcctccccg ggattctggc
    11881 tctcgaaggc ccttctcagg atctgccccg ccgtcggcgg acttcccgcc cactcgtcca
    11941 gcatcgtgat ccgggcctcg cggggcgaga aggactgata gagcaccggc acgtacgcga
    12001 tcaccagggc caggaagccg aagcccagcc ccgcctcgag caccgtcagg aagcgcgaga
    12061 gccccgtcat cggcaccacg tccccgagcc ccagcgtgaa gaaagtgctc ccgctcaggt
    12121 agaggtcggc cgggtagccg gtcatgccgc tcgatccgtg gaccgtggat cccagcgacc
    12181 aatgcagcac cgagaagccc acgatgattc cggtcgccca gacggcgaag acgaggatca
    12241 gggagatctg ggcgtagaag ctgaggaaga gctcgcggcg gttccgcgcg cgaattccgc
    12301 ggccgaagaa tttccacact ctccagagca ggcgaaagac caggcgcgag acccggacgt
    12361 cgccgggaag gcgccgcgga aggaggatcg tctcgtaggc gtcccagagc gggatcagga
    12421 ccagcagggc gcccgtgatc gcggcgagcg cgctcagtgc accgatggct gggactccgt
    12481 cgattccgat tcgagcaggg tgcgaagctt gatcaaggcg cgctgctcga gctggcggat
    12541 ccgctcgcgc gagaggccga actgcgcgcc ggtctcggcc agcgtgcgcg ggcggtcgtc
    12601 gaagaagccg tagcggaagc ggaggatcgc ctcctcgcgc tccgagagct gcttcagcac
    12661 ttcgttcacc tgctggtcgc ggagctgcag ctcgacgatc tcgtccaggg tcggcggctg
    12721 ctccacggcc tccgattcca ccaggccgtg gaaggcctcg ttcgacagat ccacgtcgag
    12781 cgaccgcacg gagtggacga gggtctccag gcgcttcgcg cgcgtcatcg gaatgcccat
    12841 gacccgcgcg atctcttcca ggtcgggcgg ccggcgccgc tccgtctcca gccggcgctg
    12901 ggtcgcgatg aagcgtcgca tcatctggag cacgtggatg gggatgcgca ccgtgcgcga
    12961 ctggttggcc accccgcgcg ccagcgcctg gcggatccac cacgtggcgt acgtcgcgaa
    13021 atggatgccg cggtccacgt cgtagcggtc caccgcggtg atcagtccga gattcccctc
    13081 ctcgaccagg tcgaggaatt ccacgccgcg gttgcgatag gcgcgcgcct gcgagaccac
    13141 gaggcggaga taggagagga tgaggcgctt gcgcgcgtcc tcgacgccga gccgcatcga
    13201 gtcccacagc gcgcgctctt cgtcgcgctg gaggatggga tagcgctgga tctctttcag
    13261 gtaccgccgc tccaggtcgc cgatgccgtg tccggcctcg tcgggaacgg acacctcggg
    13321 cgaggcggcg ccttcgggca gggtgacgcc ctggcggcgg gcctcggcca ggaagagatc
    13381 aaacgcgccc gcgtcgaaat cgggagagtg gagcagccgg tccagcgcgg cctcgtccag
    13441 ggcgccctgc gacaggccct cgcgcatggc ttcggagaga tcggagacga accgccgggg
    13501 tcccattcca gcctcgctca ggtgcggatg acgaagtcgc gagcgccttg cgccgagagc
    13561 cagcgcacgg tcacgtcgcg gacgcgcgcg gcggggggac cctggtgaag gtcgagcagg
    13621 tacgccttga gaatctcgtc cggaccctcg gcgtcgacct cgacgccgcc cgtaggcaga
    13681 ttccggaccg agcccgcgag ccctcgggcg cgagcccgct cctgggcgaa gaagcggtag
    13741 ccgaccccct gcacccttcc cgcgacgacc gccacgaagc ggacgcgccc cccatcgccc
    13801 cgggatgcgc ccgcttcacg cggcgcggtc atgcagggcc tccgacaaaa aaatcggggc
    13861 gagaggattt gaacctccga ccccctggtc ccgaaccagg tgctctacca ggctgagcca
    13921 cgccccgatc gaatcgagct ttacggaagc caaggaactc ccctcgcgga ggggcgcctt
    13981 tgccggggca gtgtagcaac ccccccgccg ccgggtcaac gggactcgcg ccgctcccag
    14041 gcatccctgc cgatcagcgg aacgaaggcg cagccgcaca gctcgcgccg ctgccatccc
    14101 gcctcggtgc ggaccgcctt caccagcatc tggttctccc ccaccccgat cggcatgacc
    14161 acgatcccgc cgggcttcag ctgctcgtcc aggaagtgcg gcgtgcgcgg ggcgccggcc
    14221 gcgatcagca cgcggtcgaa gggagccatc tcccgccacc ccaccgatcc gtcggcgacg
    14281 cggaagacga tgttcgtgta gcccatcttc gtgagccggt cgcgggcggc gaccccgacc
    14341 gatgcgatcc gctcgacggt gaagacccgg tcggccagct cggccaggat cgccgtctgg
    14401 tatcccgatc cggtgccgat ctcgagcact ttctcctcgc cgttcagctc cagcgcctgg
    14461 gtcatcagcg ccaccatgta gggttgggag atcgtctgcg ccgcgccgat cggaagcgcg
    14521 ctgtcgctgt aggccttggc ctccagcgcc tcgtccacga agaggtggcg cggcagcgct
    14581 tccatgatcc ggagcacgtg cgggtcgtgg atgccccgtt cgcgaagctg cgactgcacc
    14641 atgcgccgcc gggcggcgcc ctgatcttcc ggattcatct cactcgtcat ggatcgtgag
    14701 ccccatcgtc tcgagctcga cgatcgcctt gtagtcgttg acgtcgaatg agagcggcgt
    14761 catcgacacg tagccgttcg agaccgcgtg gaagtcgctc gcctcgtcgg ggtcccaggt
    14821 cgggttctcc ccgccgatcc aatagtacga ctttccccga gggtcggtct tctcgacgat
    14881 cacgtcgtgg aagacgcggc gcccgagccg cgcgacgcgc aggccccgga tctcggcgta
    14941 cgggaggggc gggatgttca cgttgaggca catccccggc gtgagcccgc gctggaggag
    15001 catgcggacc agcgccgtcg cgatccgccc ggccggcgcg aagtcggtcg gctcccaggt
    15061 ggcgagggag aaggcgatcg aagggattcc gaggaaggat ccctcgctgg ccgccgagac
    15121 ggtgcccgag tagagcacgt cgtttcccat gttcgggccg tgattgatcc cggagacgat
    15181 cagcttcggc gcctccttca ggaacccgtg gaccgcgagg aggacgcagt cggtcggcgt
    15241 gccgtccacg ctcaggattc ccggtccggt tttccgcagc cggagcgggc ggtgcagcgt
    15301 gagcgagtgg ctggtcgcgc tctgctcccg gtcgggggcg atgatcgtca cctcgccgag
    15361 ggggcgcatc gcctcggcca gggttcggag cccctcggcc gcgatgccgt cgtcgttcgt
    15421 gaccaggatc atcggaacag gtggcgcagc acgaagcgga cgaaccggac cgtgtcacgc
    15481 acggggcgga tgtggctccc cggcgcgttg tagagcgtgg gaatgccgac cgcggcgatc
    15541 cggaatcccg cgcgcgccgc ggccacgagg aattccgatt cgtactcgaa gcggcgcgcc
    15601 tcggggcgca cccggcgcag cacgggggcg cggatcgcgc ggtagcccga ctgcgagtcc
    15661 tcgatcaggg tgcgcgcgag gcgcgacacc caccagctcg tcacgtcgtt ggtcacccgc
    15721 cggagccagg gcatcccctc ccggctctgg aggcgcgaac cgaccaccag gtcggcgctc
    15781 tcgagcccgg ccaacagccg gggcgcccag gcgggatcgt gctgcccgtc ggcgtccagt
    15841 gctaagaccg cgtcggcgcc ccggcggacc gcctcgtcga agccggtctg cagcgcggct
    15901 cccttcccct ggttcagcgt gtgacggatc acctcggccc ccgcctcctt cgcgcgggct
    15961 cccgtttcat cggaagagcc gtcgtccacg acgaggatgg tcgcctcggg aagcgtggcc
    16021 ttcagcccgc gaaccaccgg acccacggtg tccgccgcgt cgcgcgccgg aatcaccgcg
    16081 cagacgcgca tcagcgggcg cggctccgcg cgagcgggcg ctcctgcgcg gcacggccgc
    16141 gcggcgcggg ccgcgcgctc cggatgctcc agtcgaacca ctgcgtcgga tgcgcgcgaa
    16201 cgtggccgtc gaagaagcgt gacaaggagg ccagggcctc gtccaccgtg aggccgcctc
    16261 gctccggatc gagggtgaag ggggcgtgga agcggaccgc gatctgcccg tcggcgtcgc
    16321 gccacgccgc gcccgggagc acctgcgctc ccgcgcgctg cgccagccgg agcggggcgg
    16381 cgtccacggc gacgagccgc tcgcccacgg gaatcgtctc gggagcggcg gggcgccacg
    16441 agcgatcggc gagcgccgcg atccatcccc ccgcgcggag ccgccggagc gcctcggccg
    16501 cggggcgctc gccccccagc gtgcgcacgc cgaaggcggc gcgccgcgcg cggaagaagc
    16561 gctcgaccgc ctccaggcgg tggggcgcgg agagggcctc catggggccg acctcgcgcg
    16621 ccagccacca gagcgccatc tcccaggggc cgaagtgccc gctcacgacg agggtcgggg
    16681 ccttggccgc gcgcgccgcg gcgagatgcc gcgcggcctc ggcgtccagg cggaccgcgg
    16741 ggacgcggaa tcctcccccg cgcgcggcca ggaagtcgcg cagcgcccag gcgaaggcgc
    16801 ggaaggtctc gcgagcgggg ggcgggggcc cgccgagccc gccggcgtgc cacgcgcgtc
    16861 cgacgcggtg cgccgcttcg cgcgcgcggg aagggtgcgt ggagacgtag acgtcggcga
    16921 tccacgccgc catccgatcg gtcatccaga ccggcaggcg tcgcgccgcg atcgaggcga
    16981 tgtggtagcc cagatccgtc atgctccccc acggaaacgg cgggccggcg gcgcgcggcg
    17041 ccgggccccg aaacacggac gccccggacg gttcgcgccg gggcgaggga ctgcgggaat
    17101 caaaatggtc ggggcgacag gatttgaacc tgcgaccacc tccaccccaa ggaggtgcgc
    17161 taccgggctg cgctacgccc cgacccgaag ccggcggcga cgatcggcct caagggccat
    17221 gctacacgac cggacgcggt cgggacaagt cgggggcggc cgctcgaaaa gcgccgccga
    17281 cccacgcggc agggatgccc tcggtcgcta ccgcccgcgg gcgcgcttgg ccgtgtcctg
    17341 cttcagcatg tgcagcaggg actccatgtc cttcttcagg gagcggatcg tctcctcctt
    17401 gtcgaacttg gtgaacggga gctcgatctg atccttgccg tcctgcttgt cgtccaggat
    17461 cttccgccgc gcgccggcga tcgtgaaccg ctggtcgtac aggagctgtt tgatgtcgaa
    17521 cagcacctcg atgtcccgct tccggtacat gcgcgcgccg ccgcgaccct tgcgcggacg
    17581 gagcatcttg aactgggtct cccagtagcg cagcacgtgg ggtttgacgc ccaccaagtc
    17641 gctcacttcg ctgatcgagt agtagagctt tccggtcgga agcgacttca cgggttcacc
    17701 tcctcgaaac ggttcgggcc tccggtttga gatcgcgcgt caacagctcg cgcatggccg
    17761 atcgggcact ctttccttcg tacaggatcg cgcgcacctg ctccgcgatc gggagctcga
    17821 ttccgagacg cgccgccagg tccaccgccg cctgcgcggt gccgactccc tcggcgacca
    17881 tcggcgacgc cgccagcgcc tcggcaagcg tggcgccccg tccgaccgct tcgcccaggc
    17941 gccggtttcg gctgtggcgg ctggtcgccg tcgcgatcag gtcccccatg ccggccagcc
    18001 ccgagaaggt ctccggacgg gcccccagcg cctcgccgag ccgcgccatc tcggcgaggc
    18061 cgcgcgtgag cagggcgccc ttcgtcgagt cgccgaagcc gagtccgtcg ctgatccccg
    18121 ccgcgatcgc gacgacgttc ttcagggcga cggcgatctc gacgcccagc acgtcgtcgt
    18181 tcgtgtagac gcggaacgtc tcgctggaga acgcctcctg caccgccgcg gcgcgcccgt
    18241 gatcccggca cgcggcgacg accgccgtcg ggatgccgcg cgctacctct tcggcgtggc
    18301 tcggtcccgc gagcacgacc ggatcggtgc cgagcggcgc cgccagcgtc tcctggacga
    18361 cctcgctcat ccggcggtgc gtgtccttct ccagcccctt cgtcgcgatc acgacgagcc
    18421 gcgccgcgag cgccgtcttc tccgcggcga ggcgtgaggc ggtcgcgcgc agcgcgtgcg
    18481 agggggtggc gaagagaatc gcggaggagt ccgcggcggc ctcggcgagg tcggcggtga
    18541 tcgccacggc ctcgggaatc gggacgccgg gcaggaacac gtcgttccgc cgctcacgcc
    18601 ccatccgctc ggcgagatcg gcctcgtaga cccagaggcg caccgtgtgg ccgttcttcg
    18661 tcagaagcgc cgccagcgcg gtgccccagc cccctcctcc gatcaccgcg atgttcacgt
    18721 cttggctccc ttcccgccga agctgggcgc ctcggtcccc gcggcgagac ggcggatgtt
    18781 cgggatgtgc cggatcacga cgagaagcgc gaccaggatc cccgcgacga cccgtgcgag
    18841 gcgatactcg tcgtgcggcg cgagccacag gaccagcagc ggataggcga tcgccgcgcc
    18901 cagcgacccc acggagacgc ggcgcgagag cgcgaagagg acgatccaga ccgcgagggc
    18961 gaggagtgcc gggatcggaa ggatcgcgag gaagacgccg agacttgtcg cgacgccgcg
    19021 cccgcccttg aaccgggccc agggggagaa gacgtggccc aggatggccg ccgctccggc
    19081 ggcgagggcg gtcgggacgt ggccaccgcc gaggagcgcc gccaccgctc ccttcgcggt
    19141 gtcgatcgcg aagacgagcc cgccccaccg cggccccagt ccgcggatcg cgttcgtcgc
    19201 gccgatgttt ccgctcccga ccgttcgaag gtcgacgccg cgggcgcgcg cgatccagag
    19261 gcccgaaggg atcgagccca gaaggaagcc caggatgacg gggatgggat ggaacggaat
    19321 cgcgatctcc cccgaaccgg ccccgagttc gaactactcg cttcgcctcc actccaggat
    19381 gatcggcgtt ccctggaaac cgaagcgctg tcgcaaaccg ctttccaggt aacttacata
    19441 attcttagcc cgatatcgag gattgttcac gaatagcgcg aatgagggcg ggcgggtgcc
    19501 gatctgggcg gcgtagtaga acttgaggtg ccggccctcg gcggtggcgg gcgggctgac
    19561 ccggttcagg atcgccccga tcgcctcgtt cagggtcgag gtgggaatgg agcgggcgta
    19621 ctcctcgaac accgtcaccg cggtcggcag gatcttcttc actccccggc ccgagagcgc
    19681 cgacacatag aggcgcggca cgtgctcgag gaagggatag accttcgtga actcttcctc
    19741 gatctcggcc ttcgccgcgg cgggctcctc gaccaggtcc cacttgttga agaccacgac
    19801 cagccccttc cgctgctctt cggccagcgc ggccagccgc gcgtcctgcc gcgccggctc
    19861 ccggccgacg tcgatcacca ggagcacgat gtcggcccgc gacacggcgc ggagcgcgcg
    19921 cgtgacgctg tagaactcga cggggtcgtt caccttggtc tcgcggcgaa gcccggcggt
    19981 gtccaccagg cggaaggacc tcccgtgcgc ctgcagcgtc gtgtccaccg cgtcccgcgt
    20041 cgtccccgcc accgagtcca cgaccatccg ctcctgtccg aggaggcggt tcaccagcga
    20101 ggacttcccc acgttcggcc gtccgagcac ggcgatccgg atggtgtccg gctcctcggt
    20161 ctcggcctcg cgctccggaa gcaggcgcac cacctcgtcg aggagatcac ccgagcgaag
    20221 accgtggagc gccgagaccg gcatcggctc gcccaggccg agggagaaga actccgcgac
    20281 gacctcctcg cgcgagggct ggtcggcctt gttcacgacg aggagggtcg ggcgaccgcg
    20341 gcgccgcacc cgctcggcga cctcgatgtc gagcggcgtc accccggact gcgcgtccgc
    20401 gacgaccatc gcgacgtccg cctcgtccag ggcgacctgg gtctgctgga ggatctgctt
    20461 ctggatgggg tgggcgctcg ccggatcgat cccgccggtg tccacgagca ggaacttccg
    20521 cccgctccac tccccttcgg cgtagtggcg gtcgcgcgtg agccccgaca gctcgtccac
    20581 gatggcgcgt cgctggccca ggatgcggtt gaagagggtg gacttgccga cgttgggacg
    20641 gccgacgatc gcgacgatgg ggagcaagga ggctaggccg cccgttccga acggccgctt
    20701 tcgtcgaagc cgatctgcac cggcgcgccg accgcgctct ccagatcggt gcgcgtgcgg
    20761 tcgtcgagga agatctcgcg ctccttgagg caattgggcg gaaggagcac gcgatccacc
    20821 ggcccgtgac ggagcagcgc cgcctggatg tcggcgccga ccaaaagccc gctgacggtc
    20881 accgtatcgc cgaagtactc gttcgtgacc acgcagagcg aagcgtccac cccctggggc
    20941 gggcgctcgt cgaggaggcg gcgaagcgtc ggcgccgcac tcgtgccggt cacgatggcg
    21001 acgcgctcgc gcgttccgtt ccgcgggcgg atcgcgtcgg tccccttcga ccaggcgtcg
    21061 atcgtctggc gcagcatccc gatcccgttc tccagctgcg ggaagccctc gtaggcgcgc
    21121 gcgggcggca ccgcctggtc ggccagcacg tacatctcgt cggcggcgaa gacgatccgc
    21181 gtcttgtggc gcttcaggag cgaggcctgg aaagcgtgca cttcgtccag cgcaaagacc
    21241 gcgtcttcgc gcgtgaacgt gcgcaggtcg ggcaggcggc cgcgatgctt ggtgaggccg
    21301 accggcacga ccgcgatcgt cttcacgcgg gggtagcgcg ccgccagctc ctcgatcgat
    21361 tgcgtgagca cggccccgtc gttcatcccc gggaccacga cgagctgcgt gtggaggtcg
    21421 attccccccg cggcgagccg gtcgatgtcg gcgaggatct tccccgactt gggattcttc
    21481 atcatgcgga cgcgcacggc gggatcggtg gcgtgcaccg agacgtagag cggcgagagg
    21541 cgctcgtcca ggatgcgctg gaactccgcc tcgcccaggt tggtcagcgt gatgaaattc
    21601 ccgtgcacga acgagaagac gtagtcgtcg tccttgatca acagctcgcg acggaccccc
    21661 ttggggtgct ggtagacgaa gcagaaaacg cagttgttgt ggcagcgctt gggctggcgc
    21721 gcctcgaatt cgatgccgaa ggggacgccg tactcccgga agccctccac ctcgcgctcc
    21781 gcgccggact ccgagcggac caggaggcgc aggtgctcct cggagatctg cgacccgaag
    21841 tcgatctcgt cgtggagcgg gaccccgttg atctcgagga tccgctcgcg cggaaggagg
    21901 cccaggcgct cgccgaggct tcccggctcg accttggcaa tggtgaaggg atcgctcaga
    21961 ccgatcatag gcgtgacttc gcgttcgttc ccatcgacgg tttcgtgagg tggacgccgg
    22021 gcgggcgaag aaaactggag cgggcaacgg gatttgaacc cgcgaccttc agcttgggaa
    22081 gctgacactc taccactgag ttatgcccgc tcccacaact gtgacgaagg aagactacgg
    22141 ggtttcctcc ccctggtcaa ggagcatctc ccggcggatc tggagcgcca ccgcggggaa
    22201 ttccgccggc gattccaggt cgaaccatcg cacgccggcc gttcggcgga accaggtctc
    22261 ctgccgcttc gcgagctgcc tcgtgcggag cgcgaccgtc tggaccgcct cctccagagt
    22321 aaccctcccg cgcaggtatt cgagcagctc ccggtagccg agggtgtgca ggccgggcgc
    22381 gtcgggcggg agaccggcct ccttgagggc ccgcgcctct tccaggaagc cgtcccggag
    22441 ctgctggcgg atgcggtcct cgatccgttg cgccagcgcc tggcgcgacc agcggatgcc
    22501 gaagcgccgc gccggcaggg gctcgttccg ccgctcccgg cggagggccg agatcggcgt
    22561 cccggtgaga cgatagacct cgagggcgcg gatcacgcgg acggagtcgc cgggaaagat
    22621 cttcgcgtgc gcggcgggat ccacgcgcag gagctcggcc cggagcgcct ccagcccctc
    22681 cgcctcgagg cgcgccatga gctccgcccg gacctgttcg ttccgctccg gccccgcgaa
    22741 gagcccatcc agaagcgctc gaaggtagag gcccgatccg ccgacgatga cggcggttgc
    22801 gccgcgccct tcgacctccg acaccgcggc ccgcgcctcc cgggcatagc gccccgcgct
    22861 gtaggtctcg gccggggaga gaaaggaagc catgtggtgc ggggcccgcg cgcggtcggc
    22921 ggcgccgggc gcggccgtgc cgacctcgag gcctcggtag atctggcggg aatcggctcc
    22981 gacgatctcg gcgccgatcg cctccgcgag gtggagggcg atctcgctct tcccccccgc
    23041 cgtggggccg agcaggacga cgcggcgttg cgcgggattc tcgggatgca cggaatcggg
    23101 tacggcgggc acgtcagcgg cggccgaagc ggcggtccag ctcctccatc gaaacgcgga
    23161 cgtacgtcgc gcgtccgtgc gggtcgccgt gcggcaggtc ggtggcgaag aggcggtcga
    23221 tcagcgcgcg gcgctcctcg ggacggagcg gctgtcccga gcggacggcg gcatggcagg
    23281 cgaacgactt cgcgacgcgc tcctcgggcg gctcgtcccc cccgccgtgc gcgtcctccc
    23341 ccgcgagcag cgagcggagc gcctccaccg ccgcctcctc cggaatcgac gccggaaccg
    23401 cgcggatcgc gacctgcttt tcaccgaaga gcgacacctc gtagccgagg cgccggatct
    23461 gcacttccag gaggagcaaa tggtccagct cctccgccgt cacgtcgagc acgcgcggaa
    23521 agaggaggtg ctggctgtcg gcttcctccc cgtacagccg cgcgcgcgcc tgctcgtaga
    23581 ggatgcgctc gtgggccgcg tgctgatcca cgacgaccag tcctccgcgg atcggcgcga
    23641 gcaggtagcg gtcgtggagc tgccagatcg ggatctcggt ttcgagcagg ttctcgcgca
    23701 ccgcggcctg accgccctct tccacccgtt cgggccgaag ccggggcgcg ggcgcgtatt
    23761 cttcgatgag ggtctgggtc gccgccgggg ccgcgcccag cgccgtcgcg ctttcgctcg
    23821 cccgcgggcg cggaagaagc gcgccctgcg cgccccagta cgcggccccc tcgtccacct
    23881 gagcgtccgg gcccgcggag ccgggaacgc cggccccctg ggccggggtc acgccaggag
    23941 tcgacggctc cgccggggcg ggtcgcgaca ggcggacctc cgggatgtcc agcgataccg
    24001 gaacgtggcg gcgcatcgtc gcgtccaccg cctgcttcac ggcctggaag acgcgcccct
    24061 cgtcgcggaa gcgcacctcg cgcttcgtgg gatggacgtt cacgtccacc tgcgtgggat
    24121 cgatctcgat catgaggacg gccatagggt ggcggtcgcc ggggatcagg tttccgtaac
    24181 cctggcgcag cgctgtgccc agcgccggcg agaccaccag gcggccgttc acggtcaaaa
    24241 tctgttgttc ccttccggcg cgcgcctgct cgggcgtgcc gagcacgccg cgcacgatga
    24301 tctcacccga ctcctgggcg aaggccgagg ctccctcgga cgcgccgcgt ccgtagagga
    24361 cctgccagcg ctcgaggagg tcctcggccg cggggagatc cacgtcctcg cgcccggccg
    24421 aagtgaaacg ccagtggatg gccgggtagg tcaggatgta tccccacgcc gcgcgcgcca
    24481 cggcccggat ctccgactcc cgcgatttca ggaagcgaag ccgcgccggc gtattgaaga
    24541 acaggtcctc gacgctgacg gtggttccga ccgcgcggcc gacccccgag acgccgatca
    24601 ccgtaccgcc gagcacctcg acctgggtcc cggtcagctc gctggcggag cgggtcgaga
    24661 gggagacgcg cgcgacctcg gcgatcgacg ccagggcctc gccgcggaag ccgagggtgc
    24721 ggatcgcctc gaggtcctct tcggaggaga gtttgctggt ggcgtggcgg cggaccgaga
    24781 gcagcgcgtc ctcccgcgac atgcccgcgc cgtcgtcctg gacgcggatc agcttgtcgg
    24841 ggctggcggc gacctggacc gcgatgaggc ggctccccgc gtcgatcgcg ttctcgatca
    24901 gctccttcgc gacggcggcg gggcggtcga cgacctcgcc ggcgcggatc ttggaagcga
    24961 cctccggagg cagaacccgg atggaggcca tcaggagcgg cggaggatcg gaaggtctcg
    25021 aggaaatcgg gagaggaagc gcgcgccgtc gggaccgacg accacgtcct cctccagcga
    25081 gacgaggccg tgcccgtcca cccagaggct cggctcgagg gtgtagacgt tgcccacgcg
    25141 caccggctcg aacggctcct tcccgtagcg ctcccagcgc gggcccagca cgccgccccc
    25201 gtcgtgcacc gcgcggccga gatggtggcc cagcgcgtgc gcgtactcgg ggtagccgct
    25261 gccggtcagg tgtccccgcg cgacctggtc cacttcccag ccggccgcgc ccgggcggag
    25321 cgccttggcc gagcgatcga tcgcttcgac caccgcggcg taggcgcggc gcacctcggc
    25381 cggcgcgtcg ccgttaccgg ccgcgacgta ccagagccgc tggaggtcgg agcagtagcc
    25441 gtcgaggacg attccaaagt ccatgtgcac gaggcagccg gggatcacct tctcctcgcc
    25501 cggcgcgacg tgtccgacgg cggcgtgcgg tcccaccgtc accgtcggac agtggcggcg
    25561 cggccacgcc gtgggcgcgc ccagccggtc ggcctcggcg tgcgccaggg ccgcgacctc
    25621 gcgcgccgtc atcccgatcg cgagcgcgtc gcggacgcgg tcgaagatcc cctcggtctg
    25681 ctggatggcg cgctcgatcc gcaggcgctc gtcctccagc ttcacggagc gcagatgcgc
    25741 gatgaacttc cccgcgtgcg taaggcgcct cgcgtgcggc gtccccgaaa gggtattccc
    25801 gaggtagtcg cgcaggccgg cggtgagtcc gtcggccagc gcgtcgtcct cggagacgtt
    25861 gatcccgatg cttcgcggct ttgcgccgtc caggatctcg aggagggccg ccttgggccc
    25921 ttccttgtac gtccgcacct cgtcgaagat gcccgccttc tgcacgtcgg gcgcgtcgaa
    25981 cgtggcgacg ag
//
LOCUS       DASSLZ010000089         5781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1678963, whole genome shotgun sequence.
ACCESSION   DASSLZ010000089 DASSLZ010000000
VERSION     DASSLZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678963"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(580..1335)
                     /locus_tag="VFM00_03365"
     CDS             complement(580..1335)
                     /locus_tag="VFM00_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET7497313.1"
                     /translation="MMILPSEMKEGTTVGNAQGFADVRANGRIYSRLKRALDILASGI
                     GLIILLPIFLLVAIAIKIDSRGPILYAQERVGKNRRRRPRRAADPAFGPRPGAPDRRK
                     TDCYGRVFEIYKFRSMVADAEKHTGPVWATQKDARVTRVGKILRKTRLDETPQLWNVL
                     RGDMSLVGPRPERPNFVISLARTLPDYPARCQALPGVTGLAQVKWRYDTSIETVNRKL
                     QYDLYYLRYGRLLLDLKIMAATLRVVVRGDGAL"
     gene            complement(1644..3143)
                     /locus_tag="VFM00_03370"
     CDS             complement(1644..3143)
                     /locus_tag="VFM00_03370"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET7497314.1"
                     /translation="MSRPPHASSCCASTPVRLALLTAACVLSAYLFVRPAFADVSVGA
                     SSGDFLSFEVGGRSAGMAGAHTGVATGVTAQFWNPAGLATLTQPQVGAMHANWLQDLS
                     YEWLGVAKPMGIGVGSLSVAYFHTPGIQGVDEFDNPTGEFRVYDLAVTAGVARSLARG
                     FSVGANAKMVRQSLGTISATAPAVDFGARGTIAGASIGVAVQNLGPGLSFDGSSYPLP
                     REIKMGMGRSFWKDRIQLAADYNMPSHYFDDLRVGTEIRAHPNVSLRVGYRHEFGTPE
                     DPANGLSYGVGLHFHQLELDYSMTPSDAFDDVHRLSFGYSFGSGTEEKKPEKPKPEVQ
                     PPPPPAEPGPKVIASAPAPKAPKTSSSSDGSTQASAQAPKALAAAPPKPATETVAQAT
                     PKPAPALATTATQDAPAEPAPSKPAKAKDLEYVVILPGYWSKESAQAELKALSLLGFR
                     IKDAQIEKAAGSGYFVRLTRTRSKSNADDMAASLSRMSFRASVEVASAQ"
     gene            complement(3415..4500)
                     /locus_tag="VFM00_03375"
     CDS             complement(3415..4500)
                     /locus_tag="VFM00_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497315.1"
                     /translation="MTRHRLLPSLGFAALLLAVAATAQPLGEAPSATPSGPPILTVLS
                     SPPNVSLSLSGPAEVYGRTPMDLPSSVVGRFSVLAEGGGVAKTQGVFSFNARGAAPYC
                     LSEPPGMSAGLLIRSLNYPGVPAMTVKRSLRGAPMTVAETGAIVEALRAHLRYRDRLD
                     EPGTLSGVRAIEERRVRNAWIIYGASVWGVSAIDYWIRPRFTVQEATPTRLSLVIPTL
                     DRQEVVTRSLMIPGAGQEFANHSTRGALWLAGVLAAGAGYTAAEAIVHHKRADIAYNQ
                     VLADSSGPGDQLRYLREIEVLRSDVKSAQDLRRGFAYGALAIYAANIVDALVLSIGPP
                     AAKTPPRVSATFPIGPGGAGFALTYRY"
     gene            complement(4557..>5781)
                     /locus_tag="VFM00_03380"
     CDS             complement(4557..>5781)
                     /locus_tag="VFM00_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7497316.1"
                     /translation="SLPVSTLWVLLSVTLGRTRGSRGIGAWRFYLLFQALLSAGAAAY
                     AFFRVDRAPEAPGQVFHLHGVDWAILAAILLNLILMAAKFEATHLSLPPRRRETFRPA
                     LLGVLGCSGLLSYLIFSMLATGRVDVGDIGASAAPASLLSFLLAASLIRGRIGEAHAP
                     EDRLPATATTSLLIAGGYVTWTAVLLWLTRSAGMRLAEGLLWITIGGVIVAITALTVS
                     NRLRRKLDLFLDPVWYEPRAVRRMSAKGAEAPLASAASLEDLCRLIPENARTVADVDP
                     VTLFLADPAERCFRAVGSTIAPEPGVMIPDREPLPMELRRARRPIRLTGRSDDLEYVG
                     IYVENAEQIAACSAVCAVPILGDEGLMGFLLCGARGGARQVHGEELVLLNLVSRDYAE
                     RLERLQREAARSRPL"
BASE COUNT          926 a         1929 c         2111 g          815 t
ORIGIN      
        1 ccctccggcc cgactctctc ctcacctttc ggaaccatgt cggcgggcct ccgggaggcg
       61 ccctccgcgg ctgtctctcc tcgcgtcaaa agaccagggc gtgacctgcc ggcgttcggg
      121 cgtgcgcgct tcgcgcgccg ccctcccccg catacacacg atggatcgcg tttctcagct
      181 cttcatggtg aatggatccg gggcctgcat gacgtcacgt tcgactggat cactactgaa
      241 ggagaggcga gttgatttca ccaacagcgg agcggatttc acgcacagcg gagaggcggg
      301 ttgatttcac atacaggaga gcgacgccgt gcacttcgat gggtcactta cgggagacga
      361 tcgccccgca cttcgagcct gcgctcggca ggtcgcaacg tagtcgtgga cgcgagggga
      421 ggcagccgcg gagggcgcct cccggaggcc cgccgatgcg gttaccaaac gtgaggagag
      481 agccgggccg gagggcggcc gcccgaaggg ctgcctcccc tcgcgcaacc gcgtagcgcc
      541 cggccgcccg taggcacggc ctgcgcgtgc gaaggtctct tagagcgccc cgtctccgcg
      601 cacgacgact cggagcgtgg ccgccatgat cttcagatcc agcagcagcc gtccgtagcg
      661 caggtagtag aggtcgtact gaagcttgcg gttcaccgtc tcgatcgagg tgtcgtagcg
      721 ccacttcacc tgcgcgagcc ccgtgacgcc gggcagcgcc tggcagcgcg ccgggtagtc
      781 ggggagcgtg cgcgcgagcg agatgacgaa gttcggccgc tcgggacgcg gacccacgag
      841 gctcatgtcg ccgcgcagca cgttccagag ctgaggggtc tcgtcgaggc gcgtcttgcg
      901 aaggatcttc cccacgcgcg tgacgcgggc gtctttctgc gtcgcccaga cgggaccggt
      961 gtgcttttcc gcgtccgcca ccatgctccg gaacttgtag atctcgaaga cgcgcccgta
     1021 gcagtcggtc ttgcggcggt cgggcgctcc ggggcgcggt ccgaaagcgg ggtccgcggc
     1081 gcggcgcggg cggcggcggc gattcttccc cacccgctcc tgcgcgtaga ggatcggtcc
     1141 gcggctgtcg attttgatcg cgatcgccac caggaggaag atcgggagca ggattatcag
     1201 tccgatgccg gaggccagga tgtcgagggc gcgcttcagg cgggagtaga tgcgcccgtt
     1261 ggcgcggacg tcggcgaacc cctgggcgtt gcccacagtc gtcccctcct tcatttcgga
     1321 tgggaggatc atcatgctca aggggccgtt cctcgccttc ggaacgccgg gctacagccg
     1381 gacgcgcgca agcccccgtg aggaactagg agcgacgcgg aatccggcag ctccgccatt
     1441 cccgtgcacc ggcaccaggg acggcgggag cggtggatgt caggcgagag cccgcgcgcg
     1501 agccttggcc cgccgcggac cggcccccca ccgatcaacg ataagtatag cccagtgggg
     1561 gcccaaagac aaggccgcgg ccctcttggg accgcggccc tgacgacgaa ccgatggcgc
     1621 gaagcgctcg cgcgtgagag gcgctactgc gcggaggcga cctcgacgga agcccggaag
     1681 gacatgcgcg agagcgacgc ggccatgtcg tccgcattcg atttcgaacg ggtgcgggtc
     1741 agccggacga agtatcccga ccccgctgcc ttctcgatct gggcatcctt gatcctgaat
     1801 ccgagcagcg agagcgcctt gagctccgcc tgggcactct ccttggacca gtagccgggc
     1861 aggatcacca cgtactcgag atccttggcc ttggccggct tgctcggcgc gggttcggcc
     1921 ggagcgtcct gggtggccgt cgtggcgagg gccggcgccg gcttgggcgt cgcctgggcc
     1981 acggtctcgg tggccggctt cggcggcgcc gcggcgaggg ccttgggcgc ctgcgcgctc
     2041 gcctgcgtcg agccatcgga agacgatgac gtcttcgggg cctttggcgc cggagcggac
     2101 gcgatcacct tcgggcccgg ctccgcgggc ggcggcggcg gctgaacctc gggcttcggc
     2161 ttctcaggct tcttctcctc cgtgcccgag ccgaagctgt atccgaacga aagccggtgc
     2221 acgtcgtcga acgcgtcgct cggcgtcatc gagtagtcca gctcgagctg gtggaagtgg
     2281 agcccgactc cgtacgagag gccgttggcc ggatcctccg gcgtgccgaa ctcgtggcgg
     2341 taccccacgc gaagcgaaac gttcggatgg gcgcggatct ccgtgccgac gcgcagatcg
     2401 tcgaagtagt ggctcggcat gttgtagtcc gccgcgagct ggatgcggtc cttccagaag
     2461 gagcggccca tccccatctt gatctcgcgc gggagcggat aggacgagcc gtcgaacgag
     2521 aggcccggcc cgagattctg cacggcgacg ccgatgctgg cgccggcgat ggtgccgcgc
     2581 gcgccgaagt cgacggcggg agcggtggcg gagatggtgc cgaggctctg gcggaccatc
     2641 ttcgcgttcg cgccgaccga gaacccgcgg gccagtgagc gcgcgacgcc ggcggtcacg
     2701 gcgaggtcgt agacgcggaa ctcgccggtg ggattgtcga actcgtccac accctggatt
     2761 cccggcgtgt ggaagtaggc gacgctcagc gatccgacgc cgatccccat cggcttcgcg
     2821 acgccgagcc actcatagct caaatcctgg agccagttcg cgtgcatcgc gccgacctgc
     2881 ggctgcgtca gggtcgcgag ccccgcggga ttccagaact gcgccgtcac ccccgtggcg
     2941 acgccggtgt gtgcgcccgc catgccggcc gatcgccccc ccacctcgaa cgagagaaag
     3001 tcccccgagg aggctcccac cgagacgtcc gcgaacgctg ggcgtacgaa caggtacgcg
     3061 gagagaacgc aggcggccgt gagcagagcg aggcgaacgg gcgtggaggc gcagcacgag
     3121 gaggcgtgag gcggcctgga catgcgagtt tcccccgttg atgtcgagtc tgaatcggcc
     3181 gccgtccgcc gaactccgcg aggcaggcat tgaggatttt gggtccaccc agagtcgagc
     3241 acggaacatg ccagtgcatt cgcgcattac gcacgctggt aaacgccgga actgcatgtg
     3301 ccttcgagga gacggatcgg gggtggcgtg atcgcgcaaa acgcaatcgt cgcgggaccg
     3361 gggggagtcg tattccgcac gccgccgttc gcgaagtacg cgcgcggcgt cgcgtcagta
     3421 gcggtaggtc agggcgaagc ccgcgcctcc gggaccgatc gggaacgtgg ccgagacgcg
     3481 cggcggcgtc ttggcagccg gaggtccgat cgagagcacc agcgcgtcga cgatgttcgc
     3541 ggcgtagatc gcgagtgcgc cgtaggcgaa tccccggcgg agatcctgcg cggacttgac
     3601 gtcggagcgt aggacctcga tctcgcgcag gtaccgtagc tggtctccgg gaccgctgga
     3661 atcggcgagg acctggttgt acgcgatgtc ggcgcgcttg tggtggacga tcgcttcggc
     3721 ggccgtgtac cccgcgccgg ccgcgagcac gcccgccagc cagagggcgc cgcgggtgct
     3781 gtggttcgcg aactcctggc ccgcgccggg aatcatcagc gagcgcgtga ccacctcctg
     3841 gcggtccagc gtcgggatga cgagcgagag ccgcgtcgga gtcgcctcct gcaccgtgaa
     3901 acgcgggcgg atccagtagt cgatggcgct cacgccccac acggaagcgc cgtagatgat
     3961 ccaggcgttc cgcacgcggc gctcctcgat cgcgcgcacc ccggagaggg tgcccggttc
     4021 gtcgaggcgg tcgcggtagc gaaggtgcgc gcgcagggcc tccacgatcg cgccggtctc
     4081 ggccaccgtc atcggcgctc cgcgcagaga gcgcttcacg gtcatggccg gtacgcccgg
     4141 atagttgagc gatcggatga ggagcccggc gctcatgccg ggaggctcgg agaggcagta
     4201 cggagccgcg ccccgcgcat tgaaggagaa cacgccctgc gtcttcgcga caccgccgcc
     4261 ctcggcgagg accgagaagc gccccaccac ggacgacggc aggtccatcg gcgtgcgccc
     4321 gtagacttcc gcgggccccg aaagcgacag ggacacgttg ggcgggctgg agagcacggt
     4381 gaggatgggc ggcccggacg gggtggccga aggcgcctcg cccagcggct gcgcggtcgc
     4441 ggccacggcg agcagcagcg cggcgaaacc aagcgatggg agcaagcggt gtcgggtcat
     4501 ggccacgaga cggtacgggc gagtcgcgag cctgtcaagg accgacccgg cgcgtgtcat
     4561 agcgggcggc tgcgcgcggc ctcgcgctga aggcgctcga gccgttcggc gtagtcgcgc
     4621 gagacgagat tcaagagaac cagctcttcc ccgtggacct ggcgcgctcc gccgcgcgcg
     4681 ccgcagagga ggaatcccat cagcccttcg tcccccagga tcggcaccgc gcacacggcg
     4741 gagcacgccg cgatctgctc ggcgttctcg acgtagattc cgacgtactc gagatcgtcg
     4801 gagcggccgg taagccggat cggccggcgc gcgcggcgga gctccatcgg gagcggctcg
     4861 cggtcgggga tcatgacccc cggctccggc gcgatcgtgc tccccaccgc gcggaagcag
     4921 cgctcggcgg gatccgcgag gaagagcgtg accggatcga cgtcggccac ggtgcgcgcg
     4981 ttctcgggga tgaggcggca gagatcttcc agcgacgcgg cgctcgcgag cggcgcttcg
     5041 gcacccttgg ccgacatccg gcgcacggcg cgcggctcgt accacacggg gtcgaggaag
     5101 aggtcgagct tccggcggag ccggttcgac accgtgagcg cggtgatcgc gacgatgacg
     5161 cccccgatcg tgatccagag cagaccctcg gccagccgca tcccggccga tcgcgtcagc
     5221 cacaggagga ccgcggtcca cgtcacgtat cccccagcga tcaggagcga cgtcgtggcc
     5281 gtggcgggca gccggtcttc gggagcgtgc gcctcgccga tgcggcctcg gatgagcgag
     5341 gcggcgagga ggaacgagag gagcgacgcc ggggcggcgc tcgccccgat gtcccccacg
     5401 tcgacccgtc cggtcgccag catcgagaag atgagatacg acaggagccc cgagcagccg
     5461 agcacgccga ggagcgcggg acggaacgtc tcccgccggc gcggcggcag cgagaggtgc
     5521 gtcgcctcga acttggccgc catgaggatc aggttgagga ggatcgcggc gaggatcgcc
     5581 cagtccacgc cgtgcaggtg gaagacctgc cccggcgctt ccggcgcgcg atccacgcgg
     5641 aagaaggcgt acgcggccgc tcccgccgag agcagggcct ggaagagcag gtagaagcgc
     5701 caggcgccga tcccacgcga gccccgcgtg cggccgagcg tcacggagag cagcacccag
     5761 agcgtcgaca ccggcaagga g
//
LOCUS       DASSLZ010000090        13338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_666118, whole genome shotgun sequence.
ACCESSION   DASSLZ010000090 DASSLZ010000000
VERSION     DASSLZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13338
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666118"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFM00_03385"
     CDS             <1..398
                     /locus_tag="VFM00_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497317.1"
                     /translation="GGLVILSVMSLVGALMRALPEVLKLARRDGPAKMDEVVRTGILP
                     PEKDYGQGSEGIDITLPAKLYRWSELETLLARHGTIVAASGAGLLRGAPPEEPELRAF
                     LARTEVAIAAEPGAISAGEHIVAVLRKDG"
     gene            420..1820
                     /locus_tag="VFM00_03390"
     CDS             420..1820
                     /locus_tag="VFM00_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET7497318.1"
                     /translation="MNRNVDRLGALLQAAIRIPLRAALRSYFRKIEVRNAGRIPDSGP
                     LLVIANHPATLSEVFLLSARLGRRFHFLAASFVFRPWIRGVFVRLCGTLPVYRRQDNP
                     EQTYRNEETFRACHEVFDRGGAIAIFPEGESVTDRRILPLKTGAARLALGYDAKPGRE
                     AMLAIVPVGVHFSDRTAFRSDAVLSVGEPIDLGPYRAIGASCPQEAVRALTAAMQERL
                     ESLIVNVPNQALAAFVEDVERLYLGNLRERRPGEADVRLLQRTADCLRYYQAVDPQRL
                     YSGWRRITAYRRKLRALRLNDDALRSGLPRGLAARSAARLTIGGVAGFAPAMMGVVAN
                     GLPYLATDFFAARAAPTAIEVSAGRIVAGLIFFPLFYGLAAAALRVQAGWSWPTVAGF
                     VALALPLGLFALAYIRWLRSERERLRLALMTSRRRRLVAKLRAERRALIRLFDQAREE
                     YLRATEGAGNTERPGS"
     gene            complement(1825..2247)
                     /locus_tag="VFM00_03395"
     CDS             complement(1825..2247)
                     /locus_tag="VFM00_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497319.1"
                     /translation="MANQRNVMAVVVVGALTLLVGGAGLYLGFASGRHAPTGPPVPKQ
                     PDYGPILYDMRSIRSEPDRIRFEWARFDDANAYRITVLSPSDDSLFASPALTSNAWVL
                     PTDLRRKLTAQSIYHWRVTVFSKSGSTRSSDPAAFATQ"
     gene            2324..2956
                     /locus_tag="VFM00_03400"
     CDS             2324..2956
                     /locus_tag="VFM00_03400"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET7497320.1"
                     /translation="MSVAVVLTHGIPPSRETLHQAVQAARLFVCADGAADHALAYGIE
                     PHVVIGDLDSVKEETLARFPKAEVVQDADTETTDTEKAIRYVIARGGFRQILLLGASA
                     DRLDHVIGHLSLLLRYSDRIAITLMDERCRAFVGTETTTLDLPSGTVVSFFAVGGPAN
                     GVTTQGLRYPLAQDRLELGVRDSISNVVEARPATVVVEQGRLLFFIVTDP"
     gene            complement(2937..4619)
                     /locus_tag="VFM00_03405"
     CDS             complement(2937..4619)
                     /locus_tag="VFM00_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7497321.1"
                     /translation="MPNTFSDGTSALGAAAGGALQQHFENAPIGMLLLGEDRTIVRGN
                     ETVASITGLAPAILSGSPFRRFLSTDAPLDLEDRIFEELEHSGRWLGEVEIRTSIGDT
                     SPMLVAITPVADCAPVRYVVTVFELGNQRWIEAESSRRAAELSALSAIAVATGSSTDF
                     DEMLQAAAREIVEGLEVDACWIYCRDEWSPELRLGAGATHLDPSLRLSSYMIPDPKNP
                     ALLRAAEARELVTESELLDRSIATVMHVPLLSRDDTVGVLSILSVEGEKLSTRSSDLL
                     RAIGYKIGTAIQNVRLLESVRRQQQELKEKNDELETLVSQLLEADRLKNEFLANTSHE
                     LRTPLNSIIGFLNLVIDGLCENEEERMELLNHALTSGRHLLSLINDVLDLARMEAGRL
                     QVEMGEVDLGAVLNEVAATMAVQAREKYLDLFCPPIPEGVRVSADESRLRQVFVNVIG
                     NAIKFTPKGTVTIGLETPAGAPYVEVWVRDTGIGVGEEKMARLFHKFSQGDTSTTRKY
                     GGTGLGLVIVKELVEMMGGNVRLESAGEGKGTRVTMRLARLLGGAPATGPSR"
     gene            4735..5061
                     /locus_tag="VFM00_03410"
     CDS             4735..5061
                     /locus_tag="VFM00_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497322.1"
                     /translation="MTKVTAGVLTGIVLGAAHGVVSAWGDPHTLDLFVSVLGRASQGI
                     INGVLAAYVAPGRTPAWRAMLLSGLIGVALGGLSGIPHKSWQQTLPLGAFIGVACGAA
                     AARAGR"
     gene            complement(5058..7832)
                     /locus_tag="VFM00_03415"
     CDS             complement(5058..7832)
                     /locus_tag="VFM00_03415"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7497323.1"
                     /translation="MSTRRSISARTPLLRTLPAALVAALFLSAPLAAAAPHDNDRAAR
                     GARAGKADAAIPAAPEAPVIREVLPDGLTLLMQVDHSKPLVGVCIVVNGGSRTEDPKL
                     SGLSHYYEHLIFRGGSARQDELEFRKEMQRIGEESGGYTTNDYTCYGFTAPTANLDEA
                     LWRSLDAWLALKLTQPKVDKERQVIMEEYNQGEDRPDYKVYYQIERLMFRDHPYKRDT
                     IGLKDAIMNSSLATFRTFYADRYVPNQMTLAVVGDFDPQTMRTKIERGFAPYPRGKES
                     FELGITEKPQTEFRMGVETMKTPSTWTYLGFHTAPFRGPDSPTLTVIASLLGKGTSSR
                     LYRALKEKENLVTDVSADFETRKDPGMFVVSTQMPPANEAKVFGVVRDELTRLATEPV
                     PPAELARVKAALVNDWVFAAQTPFSRAERLCVFSVMADPAIAGLWPRLIESVTAEDIR
                     RVAAATFAPNQASYSVVRPADAPSASGPSQSDVLAMVAPWTASWPASAAAAPAATASA
                     PRRETLANGVTLILNEDHSTPIVAVATYAKGGQWIEPEGLAGVSHMAATLLRRGAGSM
                     SARQISDQAELLGMRLSSGGSNDYAFLNWQAPSRNFGKAWDTYRTVLTQPNFPSDETA
                     KVRQDLIQQVQSMGDRPFDLTNLRFAEAIYRKSPYRRSVIGDETSLKKITAADLRQAY
                     RTEFSGANLVVAISGDFDSDATLALARRTLGAIPKGVPAKIGGVRDDPATEKRPIFVD
                     KEQEQITYNTGWIGCSLLDPDYPALKTGVALIGDRLFFKYVYEKGVAYRSWFYMNDRV
                     GQGTVQNEMGVSPQNWSASSGVLEDVTEYVTRPLKDEEVKRTIDKVLTRWYLGAQEND
                     AIAGRLGFYETTGLGYQFAQSYPERLRHLSASEVQAALRKYLHPETYTRVAIGKEPAK
                     TSGASMAPSH"
     gene            complement(7919..8143)
                     /locus_tag="VFM00_03420"
     CDS             complement(7919..8143)
                     /locus_tag="VFM00_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8111..8113),aa:Sec)
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HET7497324.1"
                     /translation="MNIEIRYCQEUSYLPRATSLAAELKKDLGIDATLTPGSGGVFDV
                     IADGKTIFSKKQVGRFPEVVEILGMLKQKK"
     gene            complement(8188..11232)
                     /locus_tag="VFM00_03425"
     CDS             complement(8188..11232)
                     /locus_tag="VFM00_03425"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET7497325.1"
                     /translation="MTRLYLHTYPNAFSGPHTIYAREGEEVAQNYDLRYAKAKQLGWM
                     TLDSATVDDAPATVDVNETIATLHLPRPLRPGDSARVALRFTVEIPRHFDRLGHTGEN
                     YSIAQWYPKMVVYDDEGWHPDPFHYMSEFYGDYGTFDVTMHVPDDYWVGSTGLLTRVA
                     GGDNDIPIQDQDAPRDSVTVTLRVAAADSLEGKRPQRELRIETDMARGDAEGRRVPVP
                     LGGEASFRVPRGAPVHYVYAWENRDGPHDEGDARGRARPLHRIVASRDTTIADTLRAL
                     AAEGGDADSLLPSMKSLHYHAEAVHDFAWVASPDYVRGDTTWSGIAIRSLCFRSDQKL
                     WSESKRDVVDAMRHHTETVGPYVWPQFTTTEAWCGGGAMEYPMLIMNEPDVASSLAHI
                     LDDTIAHELGHNWFYGMLGSDERAHPWLDEGFAQFIEDDYSDRKYPKGLFRWTDRLPW
                     LTAWSAFGRDELSYLSRAWARDEMPMVTPTEEFQGYRQYGVAAYSKTVMMLHALRGVI
                     GNEAFERFLHRYYDENLLRHPRPEDVRRAAEEAAGRDLGAFFHDWTETTLRLSFALER
                     GSSHGEGDEWVSIVRVRRDGGMALPVTVEARFADGSIQRTRVATVERENGASFRSRAR
                     LSSAVLDPDHDLADMNRLDNRTGIAPMRWRPLFDFPQTDEISVLYGPTIWHGNHEGMR
                     LGAWVEGRYLPMRDFPRGILGFEAAANGGTRTGDLAVRLGGWRRVGALGARGEVRVLG
                     VRDEGLSRFEVALGNRISAPGPRYPWRTWWLGLQARDRYDLRPVDARYWSPGTTVNAS
                     AGFRIETVGPHRQDDLTLELRRGSSAFGGGEPASEANYDAVRAIARHRMPLGWGSWNL
                     ALRASGGSAWRRVPREQLFDVAEKSRLDVLDRFYENDRGPLRATDHYLSEGGGGLRGY
                     AGRAALGARLAALNVDVTQARTGVFLFGDVGRVEASGLGESSRPAGPLVGRTLADAGA
                     GYGYGPARVTLPFWVSRPEHGEAPWRVRWRFSLDLAGIHPWW"
     gene            complement(11418..12752)
                     /gene="miaB"
                     /locus_tag="VFM00_03430"
     CDS             complement(11418..12752)
                     /gene="miaB"
                     /locus_tag="VFM00_03430"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET7497326.1"
                     /translation="MPGRIYMETYGCQMNVADSELMLGQLRRAGFERVEEAAHADVIL
                     VNTCAIREHAEQRIYGRLGELSRYKVRRPGVVLGVAGCMAQHLRDRLIEKVPQVDLVI
                     GPDGYRDLPALVDQAREEPTLAVRLSRIETYGDLTPARADGVRAWVSIMRGCDKFCTF
                     CIVPYVRGRERSLPAAEVIRQVEHAAAEGFKEIVFLGQTVNAYKDGDVDFAELLRRAD
                     KVEGIERIRFTSPHPADMTDRAVEAIGSCAKVMPQVHLPLQSASNTVLERMKRIYTIE
                     QYEALVAKLRAAVPDLALTTDVIVGFPGETEADFEATRTAMRRIRYDGAFLFKYSPRP
                     GAKSAAWEDDVPDAEKTRRITVLIEDQKEMSLERNAATIGSEVEVLVEGPSKKSADHW
                     FGKTRQFKTAVFRHGEERVGDTLRMRVAATSPYTLFGEGVSSAVRVSDDAAV"
     gene            12821..13213
                     /locus_tag="VFM00_03435"
     CDS             12821..13213
                     /locus_tag="VFM00_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7497327.1"
                     /translation="MDSSPEKSAARRRTVYIVDDEEMVRTVAAELLKHLGYDVELAAS
                     GEEAVAHIEKGSRPDCVLLDVVMPGMGGAEALRRIRAIAPEIRVVISSGFTDRVSSAS
                     LTDEGAAGIITKPYRMETLGSRLKEILT"
BASE COUNT         1973 a         4606 c         4599 g         2160 t
ORIGIN      
        1 ttggagggct cgtgattctg tccgtgatgt ccctggtggg cgcgctgatg cgggcgcttc
       61 ccgaggtcct gaagctcgcc cgccgggatg gcccggccaa gatggatgag gtcgtccgga
      121 cgggaatcct tcctcccgag aaggactatg gtcagggctc cgaggggatc gacatcacgc
      181 tgccggcaaa gctgtatcgc tggagcgagc tcgaaacgct tctcgcacgg catgggacca
      241 tcgtcgccgc ctccggagcg ggcctgctcc ggggggcgcc gccggaggag ccggagctcc
      301 gtgcattcct ggcacgaacg gaggtggcga tcgccgcgga accgggggcg atatccgccg
      361 gcgagcacat cgtcgcggtg ctgcggaagg atggataagc gcggcctccg gaggtacgcg
      421 tgaatcgcaa cgtcgatcgg ctgggagccc tgctccaggc cgcgatccgg atcccgcttc
      481 gcgccgcgct ccggagctac tttcgaaaga tcgaggtgcg gaacgcgggc cggattcccg
      541 actccggccc ccttctcgtg atcgcgaacc atcccgccac gctgagcgag gtctttctcc
      601 tcagcgcccg gctgggccgc cgcttccact tcctggcggc gagcttcgtg ttccggccct
      661 ggatccgcgg cgtcttcgtc aggctttgcg gcacacttcc ggtctaccgg cgacaggaca
      721 accccgagca aacctaccgc aacgaggaaa cgttccgcgc ctgccacgaa gtgttcgacc
      781 ggggcggcgc catcgctatc ttcccggagg gagagagcgt gaccgaccgc cggatcctgc
      841 cgctcaagac cggcgccgcc cggctcgcgc tgggatacga cgcgaagccc ggacgggagg
      901 cgatgctggc gatcgtgccc gtcggcgttc acttctccga ccggaccgct ttccgcagcg
      961 acgccgtgct ctccgtgggc gagccgatcg acctcggacc ctaccgggcg atcggtgcgt
     1021 cctgtccgca ggaagcggtg cgcgcgctga cggccgcgat gcaggagcgc ctggaatccc
     1081 tgatcgtcaa cgtgcccaac caggcgctgg ccgcgttcgt tgaagacgta gagcgcctgt
     1141 atctcggaaa tctccgcgag cgccgccccg gcgaggccga cgtccggctg ctccagcgca
     1201 ccgccgactg cctgcgctac tatcaggccg tcgacccgca gcgactctat tcggggtggc
     1261 ggcggatcac ggcgtatcga aggaagctgc gcgccctgcg tctcaacgac gatgcgctgc
     1321 gaagcgggct gccgcgcggc ctggccgccc ggtcggcggc acggctcacc atcggcggcg
     1381 tggccggctt cgcgccggcg atgatgggcg tcgtggcgaa cgggctcccc tacctcgcga
     1441 ccgatttctt cgctgcgcgg gcagctccca ccgccatcga ggtatccgcg gggcgaatcg
     1501 tggccgggct gatcttcttc ccgttgttct atgggctggc cgcggcggcg ctgcgcgttc
     1561 aggcgggctg gtcgtggccc acggtcgcgg gcttcgtcgc gctggcgctt cctctggggc
     1621 tattcgcgct cgcctatatc cgatggctcc gttcggagcg ggagcggctg cgcctggcgc
     1681 tgatgacctc gcgccgtcgg cggctggtgg cgaagctgag agcggaacgg agggcgctga
     1741 tccggctgtt cgatcaggcg cgagaggagt atctgcgggc gacggaaggg gcggggaaca
     1801 ccgagcgccc cggttcttga acggctactg cgtggcgaac gcggcggggt ccgaggagcg
     1861 cgtactaccg ctcttcgaga acacggtcac tctccagtgg tagatcgatt gcgccgtgag
     1921 cttccggcgc aagtcggtgg ggaggaccca ggcgttcgac gtgagcgcgg ggcttgcgaa
     1981 cagcgaatcg tccgagggcg agagcacggt gatgcggtag gcgttcgcgt cgtcgaagcg
     2041 cgcccactcg aaccggatcc ggtcgggctc ggagcggatc gagcgcatgt cgtagagaat
     2101 cggtccgtag tcgggctgct tcggcaccgg cgggccggtc ggcgcgtgcc ggccggaagc
     2161 aaagccgaga tagagccccg cgccgcccac gagcagcgtc agcgctccga ccacgaccac
     2221 cgccatcacg ttccgctggt ttgccatcgt tccctcgcgg atgctaggct gcgctcggcg
     2281 tgcccgcaag cctagaaccg accaaccgtc cccgagactc cgcatgagcg tcgccgtcgt
     2341 cctgacccac ggaatcccgc cttcgcgcga gaccctccat caagccgttc aggccgccag
     2401 gctgttcgtc tgcgccgatg gcgcggcgga tcatgccctg gcctacggca tcgagccgca
     2461 cgtcgtgatc ggcgacctgg actcggtgaa ggaggagacc ctcgcgcgct tccccaaggc
     2521 cgaggtggtg caggacgccg acaccgagac caccgacacc gagaaggcga tccgctacgt
     2581 gatcgcccgc ggcggcttcc gccagatcct gctcctgggc gcgagcgccg accgcctgga
     2641 ccacgtgatc ggccacctct cgctcctcct gcgctattcc gaccggatcg cgatcacgct
     2701 gatggacgag cgctgccgcg cctttgtcgg caccgagacg accaccctcg atctcccctc
     2761 cggcacggtc gtctctttct tcgccgtcgg cgggccggcg aacggcgtga cgacgcaggg
     2821 cttgcggtat ccgctggccc aggatcgcct ggagctgggg gtgcgggact ccatctcgaa
     2881 cgtcgtcgag gcccgccccg cgaccgtggt cgtggagcag ggccggctgc tcttcttcat
     2941 cgtgacggac ccgtagccgg cgcgccgccg aggagccgcg cgagccgcat cgtgacgcgc
     3001 gttcccttcc cctcgcccgc gctctccagc cgcacgtttc ctcccatcat ctccaccaat
     3061 tccttcacga tcacgaggcc gaggccggtg ccgccgtact tccgcgtcgt cgaggtgtct
     3121 ccctgcgaga acttgtggaa cagccgcgcc atcttctcct cgcccacgcc gatcccggtg
     3181 tcccgcaccc agacttccac gtagggcgcg ccggcgggcg tctcgaggcc gatcgtgacg
     3241 gtgcccttgg gcgtgaactt gatcgcgttc ccgatgacgt tcacgaacac ctggcggagg
     3301 cgcgactcgt ccgcgctcac ccgcaccccc tcgggaatgg gcgggcagaa gagatccagg
     3361 tacttctcgc gtgcctggac cgccatcgtg gcggcgacct cgttcaggac cgcgcccagg
     3421 tccacctcgc ccatctccac ctgaagcctt cccgcctcca tgcgggccag gtccagcacg
     3481 tcgttgatca gggagagcag gtggcgtccg ctggtgagcg cgtggttcaa gagctccatc
     3541 cgctcttctt cgttctcgca caggccgtcg atgaccaggt tcaggaaacc gatgatcgag
     3601 ttgagcggcg tccggagctc atggctggtg tttgccagga actcgttctt gagacggtcc
     3661 gcctcgagga gctgcgacac cagggtctcc agctcgtcgt tcttttcctt cagctcctgc
     3721 tgctggcggc gcaccgattc caggagccgc acgttctgga tcgcggtccc gatcttgtag
     3781 ccgatggcgc gcaggagatc ggagcttcgc gtcgagagct tctccccctc cacgctcagg
     3841 atcgacagca cccccaccgt atcgtcgcgc gagaggagcg gcacgtgcat caccgtggcg
     3901 atgctccggt caagaagctc ggattccgtg accagctcgc gggcctcggc ggcgcgcagg
     3961 agcgccggat tcttgggatc ggggatcatg taggacgaga gccggagcga ggggtcgaga
     4021 tgcgtggcgc cggcacccag gcgaagctcg gggctccact cgtcgcggca gtagatccag
     4081 caggcgtcca cctccagccc ttcgacgatc tcccgggccg cggcctggag catctcgtcg
     4141 aagtcggtgc tgcttcccgt cgccacggcg atcgccgaga gcgcggagag ctcggcggcg
     4201 cgccggctgg attccgcctc gatccaccgc tggttcccca gctcgaacac cgtgaccacg
     4261 tagcgcacag gagcgcaatc cgcgaccggc gtgatcgcca cgagcatcgg gctcgtgtcc
     4321 ccgatcgacg tccggatctc cacctcgccg agccaccgcc cggagtgctc cagctcctcg
     4381 aagatccggt cctccaagtc gaggggagcg tccgtggaga ggaagcggcg gaagggagag
     4441 ccggagagga tcgccggcgc cagtccggtg atcgacgcga ccgtttcgtt cccgcgcacg
     4501 atcgtccggt cctcgccgag caggagcatc ccgatcgggg cgttctcgaa atgctgctgg
     4561 agcgcgcctc cggccgccgc gccaagggcg gaagtgccgt ccgagaaggt attcggcatt
     4621 caatagctat cggcggaacg gcgggcgttc tcaactgctg gacccgaaag gcgcctcccg
     4681 ctatgctgcg gcgcgatcgg gtgttgcggc gcctcgagcc ggggaggttg ggcgatgacg
     4741 aaggtgacgg caggggtgct gacgggaatc gtcctcggcg cggcgcacgg cgtcgtgagc
     4801 gcgtgggggg acccccacac gctcgacctg ttcgtcagcg tgctcggccg cgcctcgcag
     4861 gggatcatca acggcgtgct cgccgcctac gtagcgcccg ggcgcacccc cgcctggcgc
     4921 gcgatgctcc tctccgggct gatcggcgta gccctcggcg ggctgagcgg catcccccac
     4981 aagagctggc agcagaccct tccgctcggc gccttcatcg gcgtcgcatg cggcgccgcc
     5041 gccgcgcgcg cgggacgcta gtggctgggc gccatcgagg cgcccgaggt cttggccggc
     5101 tccttcccga tcgccacgcg ggtatacgtc tccgggtgca gatacttccg tagcgccgcc
     5161 tgcacctccg aagcgctcag gtgccgcagc cgctcgggat agctctgcgc gaattggtag
     5221 cccagccccg tcgtctcgta gaagccgagc cgccccgcga tcgcgtcgtt ctcctgcgcg
     5281 cccaggtacc agcgcgtgag caccttgtcg atcgtccgct tcacctcctc gtccttgagc
     5341 ggacgggtga cgtattccgt cacgtcctcg aggaccccgc tcgacgcgga ccagttctgc
     5401 gggctcacgc ccatctcgtt ctgcaccgtg ccctgcccca cgcggtcgtt catgtagaac
     5461 catgagcggt acgccacgcc cttttcgtag acatacttga agaagagccg gtccccgatc
     5521 agcgccacgc cggtcttgag cgcgggatag tcggggtcga gcagcgagca gccgatccag
     5581 ccggtgttat aggtgatctg ttcctgctcc ttgtcgacga agatcggccg cttctcggtc
     5641 gccggatcgt cgcgcacgcc accgatcttg gccggaactc ccttgggaat cgcacccagt
     5701 gtgcgccggg ccagcgcgag cgtggcgtcg gagtcgaaat cgccggagat ggcgaccacc
     5761 aggttggccc cgctgaattc ggtgcggtac gcctgccgga gatccgcggc cgtgatcttc
     5821 ttgagcgacg tctcgtcgcc gatgaccgac cggcgatagg gcgacttccg atagatcgcc
     5881 tcggcgaagc ggagattcgt caggtcgaac ggccggtcgc ccatgctctg gacctgctgg
     5941 atcagatcct ggcgcacctt ggcggtctcg tcggacggga agttcggctg cgtcagcacg
     6001 gtgcggtagg tgtcccacgc cttgccgaaa ttccgggacg gcgcctgcca gttcagaaac
     6061 gcgtagtcgt tgctgccgcc cgaggagagg cgcatgccca gaagctcggc ctggtccgag
     6121 atctgccgcg cgctcatgga gcccgcgccg cgacgaagca gcgtggccgc catgtggctc
     6181 acgccggcca gcccctcggg ctcgatccac tgcccgccct tggcgtaggt cgccaccgcg
     6241 acgatcggcg tcgagtggtc ctcgttcagg atcagcgtga caccgttggc gagcgtctcc
     6301 ctgcgcggcg cggacgcggt cgcggcaggg gcggccgcgg ccgacgccgg ccacgaggcg
     6361 gtccagggcg cgaccatcgc cagcacgtca ctctggctgg ggccggaagc cgacggggca
     6421 tccgcgggac ggacgaccga gtaggacgcc tggttcgggg cgaacgtggc cgccgccacc
     6481 cggcggatgt cctcggcggt gacgctctcg atcaggcggg gccagagccc cgcgatcgcc
     6541 ggatcggcca tgacgctgaa gacgcagagg cgctcggcgc gcgagaaagg cgtctgggcg
     6601 gcgaagaccc agtcgttcac cagcgccgcc ttcacacgcg cgagctccgc gggcgggacc
     6661 ggctcggtgg ccagccgcgt cagctcatcg cggacgacgc cgaatacctt ggcctcgttg
     6721 gccggcggca tctgggtcga gaccacgaac atgccggggt ccttgcgcgt ctcgaaatcg
     6781 gcgctcacgt cggtgaccag gttctccttc tccttgagcg cgcgatagag gcgcgacgac
     6841 gtgcccttcc cgaggagcga ggcgatcacg gtgagggtcg gggaatcggg gccgcggaag
     6901 ggagcggtgt ggaagccgag gtaggtccac gtgctcggtg tcttcatcgt ctccacgccc
     6961 atccggaatt ccgtctgcgg cttctcggtg atccccagct cgaacgattc cttgccgcgc
     7021 gggtagggcg cgaagccgcg ctcgatcttg gtgcgcatcg tctggggatc gaagtcgccg
     7081 acgacggcga gggtcatctg gttgggcacg tagcggtcgg cgtagaacgt gcggaaggtc
     7141 gcgagcgacg agttcatgat cgcgtccttc aacccgatcg tgtcccgctt gtacggatgg
     7201 tcgcggaaca tgagccgctc gatctggtag tagaccttgt agtcggggcg gtcctcaccc
     7261 tggttgtact cctccatgat gacctggcgt tccttgtcca ccttcggctg cgtcagcttc
     7321 agcgcgagcc aggcgtccag cgagcgccag agcgcctcgt ccaggttcgc cgtgggcgcg
     7381 gtgaatccgt agcaggtgta gtcgttcgtc gtgtagccgc cgctctcctc gccgatccgc
     7441 tgcatctcct tccggaactc cagctcgtcc tggcgcgccg agccgccgcg gaagatgagg
     7501 tgctcgtagt agtggctcag cccggagagc ttgggatcct cggtgcggct gccgccgttc
     7561 acgacgatgc agacgccgac caggggcttg gagtggtcca cctgcatcag gagggtgagc
     7621 ccgtccggaa ggacctcgcg gatcaccggc gcttcgggag ctgccgggat cgcggcgtcg
     7681 gccttgccgg cgcgcgcgcc gcgagcggcg cggtcgttgt cgtgcggcgc ggccgcggcg
     7741 agcggggccg agaggaagag cgccgcgacg agcgcggcgg gcagcgtcct gagaagcggc
     7801 gtgcgggcgg agatggaacg gcgagtgctc atgaaccccc cttgccaaaa cgcgaccggc
     7861 cccgcgaggg gggccggtgg gtgcggccgc gtggaacgct acgagcgccg cgccgtcgtc
     7921 acttcttctg cttcaacatc ccgaggatct cgaccacctc gggaaaccgc ccgacctgct
     7981 tcttggagaa gatcgtcttg ccgtcggcga tcacgtcgaa caccccgccg cttcccggcg
     8041 tgagcgtcgc gtcgatgccc aggtccttct tcagctcggc cgccaaactg gtggccctag
     8101 gtagatagct tcattcctgg cagtagcgga tttcgatgtt cattggggac cctccctcgc
     8161 ggcgcgtcgg ccgcggatgg gccgatgtta ccaccaggga tggatcccgg ccagatccag
     8221 gctgaaccgc cagcgcaccc gccacggcgc ctcgccgtgc tcggggcggc tgacccagaa
     8281 gggcagcgtc acccgcgccg ggccgtagcc gtagcccgcc cccgcgtcgg ccagggtccg
     8341 cccgacgagc ggtccggctg gccggctcga ttcgcccagc cccgacgcct cgacgcgccc
     8401 cacgtccccg aagaggaaca ccccggtccg cgcctgcgtc acgtccacgt tcagcgcggc
     8461 cagacgcgcg ccgagcgccg cgcgcccggc gtagccgcgg aggcctcccc cgccctcgga
     8521 caggtagtga tccgtcgcgc gaagcgggcc ccggtcgttc tcgtagaagc gatccagcac
     8581 gtccaggcgg ctcttctcgg cgacgtcgaa gagctgctcg cgcgggacgc gccgccaagc
     8641 cgagcccccc gacgcgcgca gggccaggtt ccacgagccc cagccgagcg gcatgcggtg
     8701 ccgcgcgatg gcccgcaccg cgtcgtagtt cgcctcactt gcgggctccc caccgccgaa
     8761 cgccgaagag ccgcggcgca gctccaacgt gagatcgtcc tggcgatggg ggcccaccgt
     8821 ttcgatacgg aatcccgccg aagcgttgac ggtggttccc ggcgaccagt agcgcgcgtc
     8881 caccggccgg aggtcgtagc ggtcgcgcgc ctggaggccg agccaccagg tgcgccaggg
     8941 atagcgaggg cccggggccg agatccggtt tccgagcgcg acctcgaacc gggagagccc
     9001 ctcgtcccgc acgccgagca cgcgcacctc accccgggcg ccgagtgcac ccacacgacg
     9061 ccagcctccc agccgaacgg ccagatcccc cgtgcgggtt ccgccgttgg cggccgcctc
     9121 gaagccgagg atgcctcgcg ggaagtcgcg catcggcagg tagcgacctt ccacccaggc
     9181 gcccaggcgc attccctcgt ggttcccgtg ccagatcgtg gggccgtaga gcacgctgat
     9241 ctcgtccgtc tgcggaaagt cgaagagcgg ccgccagcgc atcggtgcga ttccggtgcg
     9301 gttgtccagg cggttcatgt cggcgaggtc gtggtcgggg tcgagcaccg ccgacgagag
     9361 gcgggcgcga ctccggaacg aggcgccgtt ctcccgctcg acggtcgcga cccgggttct
     9421 ctggatgctg ccgtcggcaa accgggcctc gaccgtcacg ggaagcgcca tcccaccgtc
     9481 gcggcgtacc cggacgatgc tgacccactc gtctccttcc ccgtgtgacg acccgcgctc
     9541 gagcgcgaag gaaagccgga gcgtggtctc ggtccagtcg tggaagaagg cgcccaggtc
     9601 gcgcccggcc gcctcctcgg cggcgcgccg gacgtcctcg ggccggggat gccgcaggag
     9661 gttctcgtcg tagtaccggt gcaggaaccg ctcgaacgcc tcgttcccga tcacgccacg
     9721 aagcgcgtgc agcatcatca ccgtcttgct gtaggccgcg accccgtact ggcggtagcc
     9781 ctgaaattct tccgtggggg tgaccatcgg catctcgtcg cgcgcccagg cccgcgagag
     9841 atagctcagc tcgtcacggc cgaatgcgct ccacgccgtg agccagggga gccggtccgt
     9901 ccagcggaac agtcccttcg gatacttgcg gtcgctgtag tcgtcctcga tgaactgcgc
     9961 gaagccctcg tccagccagg gatgggcgcg ctcgtcactg cccagcatcc cgtagaacca
    10021 gttgtgtccc agctcgtgcg cgatcgtgtc gtcgaggata tgggcgagcg agctggccac
    10081 gtcgggctcg ttcatgatga gcatcgggta ttccatggcg ccgccgccgc accaggcctc
    10141 cgtcgtcgtg aactgcggcc agacgtaggg gccgacggtt tccgtgtggt ggcgcatcgc
    10201 gtccaccacg tcgcgcttgg actccgacca cagcttctgg tcgctccgga aacagagcga
    10261 gcggatcgcg atccccgacc aggtcgtgtc cccgcgcacg tagtcgggcg aggcgaccca
    10321 cgcgaagtcg tgcaccgcct cggcgtggta gtggagggac ttcatcgagg gaaggagcga
    10381 gtccgcgtcc ccgccctcgg ccgcgagcgc gcgcagggtg tctgcgatcg tcgtgtcgcg
    10441 cgaggcgacg atgcggtgca gcgggcgcgc ccggccccgg gcgtcgcctt cgtcgtgcgg
    10501 gccgtcgcga ttctcccagg cgtagacata atggacgggc gctccccgcg ggacgcggaa
    10561 cgacgcctcg cccccgagcg gaacgggaac gcgccggccc tcggcgtcgc cgcgcgccat
    10621 gtcggtctcg atgcgaagct cgcgctgggg acgcttcccc tccagcgagt cggcggccgc
    10681 gacccggagc gtcacggtta cgctgtcgcg gggggcgtcc tggtcctgga tcggaatgtc
    10741 gttatcgccc cccgcgacgc gcgtgagcag gccggtcgaa cccacccagt agtcgtcggg
    10801 tacatgcatc gtcacgtcga acgtgccgta gtcgccgtag aactcggaca tgtagtggaa
    10861 gggatcgggg tgccagccct cgtcgtcgta cacgaccatc ttcgggtacc actgggcgat
    10921 cgagtagttc tcgccggtat ggcccaggcg gtcgaagtgg cgcgggatct cgaccgtgaa
    10981 gcggagcgcc acgcgcgccg agtcgccggg acggagcggg cgcggcagat gaagcgtggc
    11041 gatcgtctcg ttcacgtcga ccgtggccgg agcgtcgtct accgtcgccg aatcgagcgt
    11101 catccatccg agctgcttgg ccttggcgta gcgaagatcg tagttctgcg ccacttcttc
    11161 cccctcgcgc gcgtagatcg tgtgcgggcc cgagaaggcg ttggggtagg tgtgcagata
    11221 gagtcgcgtc aacgcgctgt cggcgccgct ccggtagacg atgacggcgt gcccgtccag
    11281 gcggtgacga ttggggtcca gcgtgacgtc gatcgtgtag tggagtccag gctggcggat
    11341 cgcgagggcg gcgcccggta ctgcgatcag gaataggaga agtgaagcga accggtgcgc
    11401 gcgaacccgg ggcgggttca gaccgccgcg tcgtcgctca cgcgcacggc ggaggagacg
    11461 ccttcgccaa acaacgtgta gggcgaggtt gctgccacgc gcatgcggag ggtatccccc
    11521 acgcgctcct cgccatggcg gaagaccgcg gtcttgaact gacgcgtctt tccgaaccaa
    11581 tggtccgcgc tcttcttgga cggcccctcg acgagcacct cgacttcgct cccgatcgtc
    11641 gccgcattcc gctccagcga catctccttc tgatcctcga tgaggaccgt aatacggcgc
    11701 gtcttctcgg cgtccggaac atcgtcttcc cacgccgccg acttggcgcc cgggcgcggc
    11761 gagtatttga agaggaaggc cccgtcgtag cggatccgcc gcatcgcggt gcgcgtcgcc
    11821 tcgaagtccg cctcggtctc tcccgggaac ccgacgatga cgtcggtggt cagcgccaga
    11881 tccggcaccg ccgcgcggag cttcgccacc agcgcctcgt actgctcgat cgtgtagatc
    11941 cgcttcatcc gctcgagcac ggtgttgctg gccgactgga gcggcaggtg gacctgcggc
    12001 atcaccttgg cgcaggagcc gatggcttcc acggcgcgat cggtcatgtc ggccggatgc
    12061 ggcgacgtga agcggatgcg ctcgatgccc tccaccttgt cggcgcggcg gagcagctcg
    12121 gcgaaatcga cgtcgccgtc cttgtaggcg ttcaccgtct ggccgaggaa gacgatctcc
    12181 ttgaagccct ccgcggcggc gtgctccacc tgccggatca cctcggcggc gggaaggctg
    12241 cgctcacggc cgcgcacgta ggggacgatg cagaaggtgc agaacttgtc gcagccgcgc
    12301 atgatgctga cccacgcgcg cacgccgtcg gcgcgcgccg gcgtgaggtc gccgtaggtc
    12361 tcgatccggg agagccgaac ggccagcgtc ggctcctccc gcgcctggtc caccagcgcg
    12421 gggaggtcgc ggtagccgtc ggggccgatg accaggtcca cctgcgggac cttctcgatc
    12481 agccggtccc ggaggtgctg cgccatgcat cccgccaccc ccagcacgac ccccgggcgg
    12541 cgcaccttgt agcgggaaag ctcgcccagg cgcccgtaaa tgcgctgctc ggcgtgttcc
    12601 cggatggcgc aggtgttgac caggatgacg tcggcgtggg ctgcctcttc gacccgctcg
    12661 aagccggcgc ggcggagctg ccccagcatc agctcggagt cggccacgtt catctggcag
    12721 ccgtaggtct ccatgtagat tcggccgggc atggggtgtc attataaaga gacggcgggg
    12781 ggaggtcgag ccggcaggga cccattttgg ggagggccgc ttggattcca gtcccgagaa
    12841 aagcgccgcg aggcgtcgca cggtctacat cgtggacgac gaggagatgg tccggaccgt
    12901 ggccgcggag ctgctgaagc acctcggcta cgacgtggag cttgccgcga gcggcgaaga
    12961 agcggtcgcg cacatcgaga agggcagccg ccccgactgc gtcctcctcg acgtcgtcat
    13021 gcccggcatg ggcggcgccg aggcgctccg ccgcatccgc gccatcgccc ccgagatccg
    13081 cgtcgtcatt tccagcgggt tcaccgaccg ggtgtcgtcc gcctccctca ccgacgaagg
    13141 cgcggccggg atcatcacga agccgtatcg gatggagacg ctggggtcga ggttgaagga
    13201 aatcctgacg taggggacat cccccgaacc acgactacta gtgcgtcgtc tgctcgttta
    13261 cctccaggct gccgaccatc cggtcgaacg tggcctgaag cgcttcgaaa tcacgggtcg
    13321 gtgccagcgc gaggagat
//
LOCUS       DASSLZ010000091         3705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_540036, whole genome shotgun sequence.
ACCESSION   DASSLZ010000091 DASSLZ010000000
VERSION     DASSLZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540036"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..2402)
                     /locus_tag="VFM00_03440"
     CDS             complement(111..2402)
                     /locus_tag="VFM00_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7497328.1"
                     /translation="MIARRQHPRLMRYSFAALAMCVLAWFEIGTWPLFRVSPFLPLAV
                     GVVVAATLAGPGPGFFATALGLVASQLLPRTGAAGALAYAPLVAFVGLGAAVSLLSLT
                     GHVSRTRMRKILEGVSDGCAFLGTDWRYRFVNRAAADLAGRSPEELVGRGVSEVFPHV
                     GDLPALRELRRAMDDGVPGRLETWYPPHGRWYETNLYPADEGVMIIVRDVTDRRGAEE
                     ALRESERRLRAIFEQSIAGIAQTDLEGRFVLVNERYCEIVGQSRAELSGRSLPDLIHP
                     DDRDRHAALFEELLAAGRDFVAEERYVRPNGAVVWVRKQVSAVRDAEGAPRFCFAIVE
                     EITERKHAESEKDEALQRERAARAEAESANRSKDDFLAVVSHELRTPLTAIVGWLRLM
                     RVGAVDVSTSSRALETVDRNAGILVRLVEDLLDASRIASGKLKLEVRPTTVSEAIRAA
                     LEIVRPAAEAKRIRIVARLSHGTDLVLGDPARLQQVVWNLLSNAVKFTPADGTVEVVS
                     GPSNGSVAISVSDTGPGIGPELLPRIFERFRQAESGQGRRHSGLGLGLAIVRNVVEMH
                     GGTVRAESGGPGAGARFTVLLPPYRGTEPTRAERTPALPSRNAGAPRRPDAVRATAAP
                     APSERDAPSLAGTRILLVEDEEDTRAVVTRILAWRGGEVRACASAREAREAFEAWPPD
                     VLVSDIGMPEESGYSLIEGIRKAERLSGAHTPAIALTAYAQETHRLRALASGYDAHVP
                     KPVDPVELVRMVAQVRGPVPLRR"
     gene            2746..>3705
                     /locus_tag="VFM00_03445"
     CDS             2746..>3705
                     /locus_tag="VFM00_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET7497329.1"
                     /translation="MARAFWKGSISFGLVEIPVTLRPALKSDEMSFTLLNRKDFSPVG
                     YKRYDKKTGREVPWDEIVRGYEYEPDEYVVLSDEELRAANAKATQTVEIVEFVDGSEI
                     DPLYFDTPYYVEPQKKGSKSYGLLREALQKSGKVGIANVVLRTRQHVAALLVRGDALV
                     LNLLRYAHEIRPAADLDVPAKSAGASPREVRMAEQLIEGMTATWKPGEFKDEYRDDVM
                     ALVKKKIKAHQTHTIVEPEKGGEAPVRAKEVVDLMPLLKQSLERRHGGKAGPAKARAS
                     RAAEEETAPEPARRSSKRASASRGTAQRSHTRTGTGTRAASRGG"
BASE COUNT          601 a         1283 c         1341 g          480 t
ORIGIN      
        1 agcgagagcg agggggccgg gcgaggacaa gcgcccggag cgttgcctcc cctcgcgcca
       61 aaacgcgttg cgcccggccg cccgtagaga agagatccgc gtgagacgaa tcaccgccgc
      121 aaaggcaccg gccctcgcac ctgcgccacc atccgcacca gctccaccgg atccaccggc
      181 ttgggcacgt gggcgtcgta ccccgacgcc agtgcgcgca gccggtgcgt ctcctgggcg
      241 taggccgtga gcgcgatggc cggcgtgtgc gcgccgctca accgctccgc cttacggatc
      301 ccctcgatca gcgaatagcc ggactcctcg ggcatgccga tgtcggagac cagaacgtcc
      361 ggaggccacg cttcgaaggc ctcgcgcgcc tcgcgcgccg aggcgcacgc ccgcacctcg
      421 ccgccgcgcc aggccaggat gcgcgtcacg acggcgcgcg tgtcctcctc gtcctcgacc
      481 aggaggatgc gcgtgcccgc gagggaagga gcgtcccgct ccgagggagc gggcgccgcc
      541 gtcgcgcgga cggcatcggg ccgcctgggc gctccggcat tccgggaggg aagggctggg
      601 gtccgctccg cgcgggtcgg ctcggtgcct cgatacgggg ggaggaggac ggtgaatcgc
      661 gcgcccgcgc cgggaccgcc gctctccgcg cgcacggtgc cgccgtgcat ctcgaccacg
      721 ttgcgcacga tggccagtcc caggcccagc ccggagtgcc tccggccctg gccgctctcg
      781 gcctgccgga accgctcgaa gatccgcggc aagagctcgg gcccgatccc cggccccgtg
      841 tcggacacgg agatcgcgac ggagccgttc gacggccccg agacgacctc cacggtcccg
      901 tccgcgggcg tgaacttgac cgcgttcgaa agcaggttcc agacgacctg ctggagcctc
      961 gccgggtcgc cgagcaccag gtccgtgcca tgcgacagcc gcgcgacgat gcgaatccgc
     1021 ttggcttccg cggcgggccg gactatctcg agcgccgccc ggatggcttc ggagacggtg
     1081 gtggggcgca cctccagctt cagcttgccc gacgcgatgc gcgaggcatc gagcaggtcc
     1141 tcgaccagcc gcaccagaat ccccgcgttg cgatccacgg tctccagggc gcgcgaggag
     1201 gtggagacgt ccacggcgcc gacgcgcatg agccggagcc agcccacgat ggcggtgagc
     1261 ggcgtgcgca gctcgtggga gacgaccgcc aggaagtcgt ccttggaacg gttcgcgctc
     1321 tcggcctcgg cccgcgcggc gcgctcccgc tggagcgctt cgtccttctc ggactccgcg
     1381 tgcttgcgct cggtgatctc ttcgacgatg gcgaagcaga accggggcgc gccctcggcg
     1441 tcgcggacgg cggagacctg cttgcgcacc cagaccacgg cgccgttcgg gcgcacgtag
     1501 cgctcctcgg cgacgaagtc gcggcccgcg gcgagaagct cttcgaagag cgcggcatgg
     1561 cggtcgcggt cgtcgggatg gatcagatcg ggcagggaac ggcccgacag ctcggcgcgg
     1621 ctctggccga cgatctcgca gtagcgctcg ttcaccagga cgaagcggcc ctcgaggtcg
     1681 gtctgggcga tgcccgcgat cgactgctcg aaaatggcgc gcaggcggcg ctcgctctcg
     1741 cgcagcgcct cctcggcgcc gcggcggtcg gtgacgtcgc gcacgatgat catgacgcct
     1801 tcatccgcgg gatacaggtt cgtctcgtac cagcgcccgt ggggcgggta ccaggtctcg
     1861 agacgtccgg gcactccgtc gtccatcgcc cggcgcagct cgcgcagcgc gggcaggtcg
     1921 ccgacgtgcg ggaacacctc ggaaacgccg cgccccacga gctcctcggg cgagcggccg
     1981 gcgagatcgg cggcggcgcg gttcacgaac cggtaccgcc agtcggtacc gaggaacgcg
     2041 catccgtcgg agacgccctc gaggatcttc cgcatgcggg tgcggctcac gtggccggtc
     2101 aggctgagaa ggctgaccgc ggcgcccagt ccgacgaagg ccacgagcgg ggcataggcc
     2161 agtgctccgg cagcgcccgt tcgggggagg agctggctgg cgacgaggcc cagcgccgtg
     2221 gcgaagaatc ccggaccggg gccggccagc gtcgccgcga cgacgacgcc gacggcgagc
     2281 ggcaggaacg gggagacccg gaacaggggc caggtaccga tctcgaacca ggcgagcacg
     2341 cacatggcca gcgcggcgaa ggagtagcgc atgagacgtg ggtgctgccg tcgagcgatc
     2401 atggtggcgc tccgtggaaa ttcctacgtc ggccccatcg cgacatttcg agccgcttcc
     2461 atgaagcact aacatttctc atgccatccg gagatccgag gaggacgtag gcgggcgggc
     2521 gggtggaact ctcggcaggg gcggagttga gagcgcttgc aggaccttct ctgcgtgccc
     2581 aggactctgc gggacgtgca acaacgggca ctccttgtaa gtggcgtcct tttcatgagg
     2641 ggcggaccgt ccgggcgtga agggcatctc gtgaccgcga acggttggct cgggttctgc
     2701 acctgcggta cgcggggcgg ggtgcccaaa cgaggagaaa cccctatggc tcgcgcattc
     2761 tggaaaggca gcatcagctt cggcctggtc gagatcccgg tgacgctccg gccggcgctc
     2821 aagagcgacg agatgagctt cacgctcctg aaccggaagg acttctcgcc ggtcgggtac
     2881 aagcggtacg acaagaagac cggccgcgag gtcccctggg acgagatcgt tcgcggctac
     2941 gaatacgaac cggacgagta cgtcgtgctg agcgacgagg agctgcgcgc ggcgaacgcc
     3001 aaggcgaccc agaccgtcga gatcgtcgaa ttcgtggacg gctcggagat cgatcccctc
     3061 tacttcgaca cgccgtacta cgtcgagccg cagaagaagg ggagcaagag ctacgggctc
     3121 ctgcgcgagg cgctccagaa gagcggcaag gtcggcatcg ccaacgtggt gttgcgcacg
     3181 cggcagcacg tggccgcgct cctcgtccgc ggcgacgcgc tcgtgctgaa cctgctccgc
     3241 tacgcgcacg agatccgccc cgcggccgat ctcgacgtcc ccgcgaagtc agcgggggcc
     3301 tccccccgcg aggtgcgcat ggccgagcag ctcatcgagg gcatgacggc gacctggaag
     3361 ccgggcgagt tcaaggacga gtatcgcgac gacgtgatgg cgctggtgaa gaagaagatc
     3421 aaggcccacc agacgcacac catcgtggag cccgagaagg gcggcgaggc gccggtccgc
     3481 gcgaaggagg tcgtcgatct gatgccgctc ctgaagcaga gcctggagcg ccgccacggg
     3541 gggaaggccg ggcccgcgaa ggccagggcc tctcgcgccg cggaggagga gaccgcgccc
     3601 gaacccgccc ggcgctcctc gaagcgtgct tcagcgtccc gcggcaccgc tcaacgctcg
     3661 cacacccgaa ccggcacggg cacgcgcgcc gcgtcgcgcg gcggc
//
LOCUS       DASSLZ010000092         7411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1680110, whole genome shotgun sequence.
ACCESSION   DASSLZ010000092 DASSLZ010000000
VERSION     DASSLZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680110"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..534)
                     /locus_tag="VFM00_03450"
     CDS             complement(94..534)
                     /locus_tag="VFM00_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor"
                     /protein_id="HET7497330.1"
                     /translation="MPSKATTQRARRDLKQGKKPSTAAGEFVREEMHHIREGKHGARS
                     TEQAIAIGLSKARRAGVPLKPPRKGQASERTRQSAERAYEEGQGERTPRAPSPRRSQA
                     TRRALKREPHRAASHRALSSQARSAARRRSGRTRATAGATRQGG"
     gene            complement(547..1101)
                     /locus_tag="VFM00_03455"
     CDS             complement(547..1101)
                     /locus_tag="VFM00_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET7497331.1"
                     /translation="MGKIAMIIADLFEDSEFRVPFDRLKEAGHEITVIGSEAGKRIEG
                     KQHKEVVTADRGIGDVSADEFDAIVIPGGYSPDKLRMDPRMVAFTRAFFDAGKPVAAV
                     CHAPWMLAEAGIARGRTVTSWPSIKTDLVNAGANWVDRTVVEDGNLITSRKPDDLEAF
                     SDAIIRHLDRAPATREPVAARDRR"
     gene            complement(1242..3071)
                     /locus_tag="VFM00_03460"
     CDS             complement(1242..3071)
                     /locus_tag="VFM00_03460"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HET7497332.1"
                     /translation="MNPDVEQTGAGTSLTTNTYLLEWVAETARRCQPDTIEWCDGSEA
                     EKRRLLDRAVAQGILIPLNQKKRPGCYLHRSDPKDVARVEHLTFICTPTKEEAGPTNN
                     WMAPPDAYAKLATMFEGSMRGRTMYVVPYVMGPLGSPFSKVGVEITDSVYVALSMRIM
                     TRMGKQALDLLGDSNDFSRGVHSMLDSNPERRFICHFPEDNTVWSVGSNYGGNVLLGK
                     KCLALRLGSYLGRRENWLAEHMLILGVESPEGQTTYVAAAFPSACGKTNFAMLIPPRR
                     FQGWRVWTVGDDIAWMRVGHDGRLWAINPEAGYFGVAPGTSAKSNPTAMRIVERDTIF
                     TNVAMTDDGDVWWEGMTEKAPDGLTDWLGQKYDPASGKPAAHPNSRFCSPATNNPYLS
                     KRINDPEGVPISALIFGGRRASTIPLVLQSFNWIHGVYMGATMGSETTAAATGKVGVV
                     RRDPMAMLPFCGYNMGDYFCHWLEMRSRIPYPPKIFMVNWFRKDPEGRFVWPGYGENM
                     RVLKWIVDRALGSVGGRETPVGWVPREGDLDLSGLSIASEDFMEATAVRPEEWSEEVR
                     SQGEFFRTLDPYMPKALELQRELLAESVAMGTPEGRARTADRT"
     gene            3226..3747
                     /locus_tag="VFM00_03465"
     CDS             3226..3747
                     /locus_tag="VFM00_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET7497333.1"
                     /translation="MGYIQRTLIAGESVLYRAHLHWVVLFGHALTGALGMLLGLGLVA
                     GTFITTGNDLGPRGGTAAAIGGVLLFGAGFAVLLIGILKRAATEIAVTNKRVLIKTGF
                     FGRKTIEIFLSKIESVVVDETFLGKTLGFGTVTVRGTGGTPERFDRVADPLEFRRQVQ
                     TQIEGSPTQIRTA"
     gene            3774..4472
                     /locus_tag="VFM00_03470"
     CDS             3774..4472
                     /locus_tag="VFM00_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497334.1"
                     /translation="MRSLAPLVMTIALLTGFAAGAAASPAGYVVDWASVRDKPPAPRE
                     ESQGLYTCSVQRDVPDSASRVLAIAECTYPRGDLLAALASQKGYEFGSDTVGVPVWWS
                     KGKNERRVPYAATRAALEHYLKLTQKYRDREYREPGMQPIFTSALTYRASAALEEDVT
                     VGDSTYRDVWVAHIHLSWTYDDGTFLPFVSSHRTVVLSRKGEVLAVDGDGQAVENVEM
                     SNNRAVGRERRLMR"
     gene            complement(4492..4968)
                     /locus_tag="VFM00_03475"
     CDS             complement(4492..4968)
                     /locus_tag="VFM00_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7497335.1"
                     /translation="MANVKRIPEGYTTVTPSITLRDTAKAIEFYKRAFGAEERGIMRG
                     PDGKVMHAEIKIGNSIVMMNDEVMGSRSAETQGGSPVSFYLYFEDCDAAYQRAVTAGA
                     KGDMAPVDMFWGDRMGHVIDPFGHRWNVATHVKDVTPEEMKRGQDEFLKQMASREA"
     gene            5065..5775
                     /locus_tag="VFM00_03480"
     CDS             5065..5775
                     /locus_tag="VFM00_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497336.1"
                     /translation="MIRDFLLGPPARALRGAVRDGILEAPVEYGLFLLGWALWLLDAL
                     IRIPVLAREWSAGPRPRVVPVRVVPLQRSRADPDTASARARAVLTQAGALLDPHGVRL
                     NVQSVNPVPLPDDVPPPECGLRALTGRFFSWASSRAAGAPCLTVYFVEDLGPLAGCAV
                     PGADWIVAELGTDGTTVVHELGHLADLWRHHPDPDSVMTDRPGGTHDRISRWQAALIR
                     TSRFALPVSPRRPKRGGS"
     gene            complement(5741..6286)
                     /locus_tag="VFM00_03485"
     CDS             complement(5741..6286)
                     /locus_tag="VFM00_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497337.1"
                     /translation="MVGCATLREAAALRRVEFRLDRVSDAEVARIRLDPLPTYRSLTA
                     AQVARLGLGVASKDVPFGFTLHLEGRNPQSNQVAARMIALAWSYRVDDREVLSGRLDQ
                     PLRFPPGQPVDVPIAIEFNLYRFFSGDARTLFETAAALAGQAGGRHDVEVQLTPRIDT
                     PIGPMDYPTPITIRLASADGG"
     gene            complement(6333..6668)
                     /locus_tag="VFM00_03490"
     CDS             complement(6333..6668)
                     /locus_tag="VFM00_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497338.1"
                     /translation="MVNVSDAVDKVTNRPERTEGGVAKAIEKQTSKMPSDWFLIAAVG
                     AIGASLAMQVAGSKHKSLFFGQWVPSILLLGVYNKIVKVAGSDRFDQGGTVGRPDMSR
                     PGMTTAGAR"
     gene            complement(6885..7085)
                     /locus_tag="VFM00_03495"
     CDS             complement(6885..7085)
                     /locus_tag="VFM00_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET7497339.1"
                     /translation="MADAMRRLRRSRNRMIAGVCGGIAEWLGWDVTVVRVLYVVVSIV
                     SYAFPGTLVYIILWIVMPGPDS"
     gene            complement(7088..>7411)
                     /locus_tag="VFM00_03500"
     CDS             complement(7088..>7411)
                     /locus_tag="VFM00_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497340.1"
                     /translation="SLADLESANGRPFRLSGFAWDYAGAVTSWEGGALDSLLPGVRLY
                     LEPRIEDRAGAPYRHVQGDHEFPSDNRDMRALEPRIYQIFVDFGTDGSTSTPGDAGGH
                     APGGR"
BASE COUNT         1107 a         2539 c         2508 g         1257 t
ORIGIN      
        1 gccgtgcccg ggagcgcgag cagcgtggcc gcgaggatca gcgcggcggt cgccaaggcg
       61 aggctgccgc gcctgaaaag aacgttccgc atctcatcct ccttgccggg tggccccggc
      121 ggtggcgcgc gtccggcccg accgccgccg cgctgcgctt cgtgcctggc tcgagagtgc
      181 ccggtgcgag gcggcccgat gcggttcccg cttcagggcg cgtcgtgtcg cttgcgagcg
      241 gcgcggcgag ggcgcccggg gcgtccgctc cccctggccc tcttcgtacg cccgctcggc
      301 gctctgtcgc gtcctctcgc tggcctgccc ctttcgcgga ggcttcaagg gcacgccggc
      361 gcggcgcgct ttcgagaggc cgatcgcgat cgcctgctcc gtggaccgtg ctccgtgctt
      421 tccctcgcgg atatggtgca tctcttcccg aacgaactcg cccgccgcgg tggagggctt
      481 cttgccctgc ttcagatcgc gccgggctct ctgggtcgtt gctttcgatg gcatggcgat
      541 tcctcctcag cgcctgtcgc gggccgcaac cggctcccgc gtggcgggcg cgcgatcgag
      601 atggcggatg atggcgtcgg agaaggcttc cagatcgtcc ggctttcgcg aggtgatcag
      661 gttgccgtcc tcgacgaccg tccgatccac ccagttcgcc ccggcgttca cgaggtcggt
      721 cttgatcgaa ggccacgagg tcaccgtcct gccccgcgcg attccggcct cggcgagcat
      781 ccagggggcg tggcagacgg ccgcgaccgg cttccccgcg tcgaagaacg cgcgggtgaa
      841 cgcgaccatg cgtgggtcca tgcggagctt gtcgggcgag tagccgcccg gaatgacgat
      901 ggcatcgaac tcgtcggcgg aaacgtcgcc gatgcctcgg tcggccgtga cgacctcctt
      961 gtgctgtttc ccttcgatcc gctttccggc ttccgacccg atgacggtaa tctcgtggcc
     1021 ggcctcctta aggcggtcga aggggacgcg gaactctgag tcctcgaaca gatccgcgat
     1081 gatcattgcg attttaccca tggctccctc cttggggtgg ggtccagagc caaaaaggtg
     1141 catcgcgcgt gccgggcggc acccgtgggg agagccggac atgacgaaga aggcgcgccg
     1201 gacgcgccga cgcgccttat ggctgcgggg atctcggtag gctaggtgcg atcggcggtt
     1261 cgagcccgcc cctcgggcgt gcccatggcc accgactcgg ccaggagctc gcgctgaagc
     1321 tcgagcgcct ttggcatata gggatccagg gtccggaaga actccccctg cgatcgcact
     1381 tcctcgctcc actcctcggg gcgcaccgcg gtggcctcca tgaagtcctc gctcgcgatc
     1441 gagagcccgg agagatcgag gtccccctcc cgcggcaccc agccgaccgg ggtctcgcgc
     1501 ccgcccaccg agccgagcgc gcgatccacg atccacttga gcacgcgcat gttctcgccg
     1561 taccccggcc agacgaagcg gccctcggga tccttgcgga accagttcac catgaagatc
     1621 ttcggcggat agggaatgcg ggagcgcatc tccagccaat ggcagaaata gtcccccatg
     1681 ttgtagccgc agaaggggag catcgccatc gggtcgcgcc gcacgacgcc aaccttgccg
     1741 gtggccgccg ccgtggtttc cgaccccatc gtcgcgccca tgtagacccc gtggatccag
     1801 ttgaacgact ggaggacgag cggaatggtg ctggcccgcc gcccgccgaa gatcagcgcc
     1861 gagatcggca ctccctcggg atcgttgatc cgcttggaca ggtacggatt gttcgtggcg
     1921 ggggagcaaa agcggctgtt cggatgcgcg gccggcttgc ccgaggcggg atcgtacttt
     1981 tgtcccagcc agtcggtcag tccgtccggc gccttctcgg tcattccctc ccaccatacg
     2041 tccccatcgt ccgtcatcgc gacgttcgtg aagatggtgt cgcgctctac gatccgcatg
     2101 gcggtgggat tcgacttggc gctggtgccg ggtgccaccc cgaagtagcc ggcctcggga
     2161 ttgatggccc agagccgccc gtcgtggccc acgcgcatcc aggcgatatc gtcgcccacg
     2221 gtccacacgc gccacccctg gaagcgccgc ggcgggatca gcatcgcgaa gttggtcttg
     2281 ccgcaggcgc tggggaaggc ggcggccacg tacgtggtct gcccctcggg gctctcgacg
     2341 cccaggatca gcatgtgctc cgcgagccag ttctcgcgcc gccccaggta ggaccccagc
     2401 cgcagcgcca ggcacttctt gccgagcagc acgttgccgc cgtagttgct gcccaccgac
     2461 cagaccgtgt tgtcctcggg gaagtggcag atgaagcgcc gctccgggtt gctgtccagc
     2521 atggagtgga ccccgcggct gaaatcgttg gagtcgccca ggagatcgag cgcctgcttg
     2581 cccatgcgcg tcatgatccg catcgagagg gcgacgtaga cgctgtcggt gatctcgacg
     2641 ccgactttcg agaaagggga gcccagcggg cccatcacgt agggcaccac gtacatcgtg
     2701 cggccgcgca tcgagccctc gaacatcgtg gccagcttgg cgtaggcgtc gggaggagcc
     2761 atccagttgt tggtggggcc cgcttcctcc ttcgtcgggg tgcagatgaa agtgaggtgt
     2821 tcgacgcgcg cgacgtcctt gggatcggag cgatggaggt agcagccggg gcgtttcttc
     2881 tggttcaggg ggatcaggat gccctgcgcc acggcgcggt cgagcaggcg gcgcttctcg
     2941 gcctccgagc cgtcgcacca ttcgatcgtg tcgggctggc accggcgggc ggtctccgcg
     3001 acccactcga ggaggtaggt gttcgtcgtc agggaggtac cggccccggt ttgctccacg
     3061 tccggattca tggactcatc ctcgctcgag agaggaaaag cgcgtccagc ttatagctac
     3121 ccattataga cccaactctg cccgatgggc gcggctccgc ccagtcgcca ggagtcccat
     3181 tgtggccgca ccggccgcgg cgttaccctg ctcgcgggaa cctccatggg atacatccag
     3241 agaacgctca tcgccggcga gtcggtgctc taccgggccc acctccactg ggtggtcctg
     3301 ttcggacacg ccctgaccgg cgctctggga atgctcctcg gcctggggct ggtcgccggc
     3361 acgttcatca cgaccgggaa cgacctcggc ccccgcggag gcacggccgc cgcgatcggc
     3421 ggcgtgctgc tcttcggtgc gggcttcgcc gttctgctga tcggcattct gaagcgggcg
     3481 gccaccgaga tcgcggtgac gaacaagcgg gtgctcatca agaccggctt cttcggccgg
     3541 aagacgatcg agatcttcct ctccaagatc gagagcgtgg tcgtggacga gacgtttctc
     3601 ggaaagaccc tgggcttcgg caccgtgacg gtgcgcggaa ccgggggaac gcccgagcgg
     3661 ttcgaccggg tggccgatcc gctcgaattc cgcaggcagg tgcagaccca gatcgagggg
     3721 agtcccaccc agattcgcac cgcctgatct ccggccgttc ctacggaggt atcgtgagat
     3781 cgctcgctcc gctcgtgatg acgatcgcgc tcctgaccgg cttcgccgcc ggggcggcgg
     3841 catcgcctgc cggatacgtc gtcgattggg cgtcggtgcg ggacaagccc cccgcgccgc
     3901 gggaggagtc ccaggggctc tacacctgct ccgtccagcg cgacgtaccc gattcggcct
     3961 cccgcgttct cgcgatcgcc gagtgcacct atccgcgcgg cgacctcctg gccgcgctgg
     4021 cttcgcagaa gggctacgag ttcggctcgg acaccgtggg cgtgccggtg tggtggagca
     4081 aagggaagaa cgagcgccgc gtcccctacg ccgccacgcg ggccgctctg gagcactatc
     4141 tcaagctcac gcagaagtac cgtgatcgcg agtatcgcga gccggggatg cagccgatct
     4201 tcaccagcgc gctgacctat cgggcttccg ccgcgctcga ggaagacgtc acggtcggag
     4261 actccaccta cagggacgtg tgggtcgcgc acatccacct gagctggacg tacgacgacg
     4321 gaaccttcct gccgttcgtc tcgtcgcacc gcacggtcgt gctctcgcgg aagggggagg
     4381 tcctggcggt ggatggggac gggcaggccg tcgagaacgt ggagatgtcg aacaaccggg
     4441 ccgtggggcg cgagcggcgg ctgatgcgct agccgccgcc cgccccgttc gtcacgcttc
     4501 gcgcgacgcc atctgcttca ggaactcgtc ctgcccccgc ttcatctctt cgggggtcac
     4561 gtccttcacg tgcgtcgcca cgttccagcg atgaccgaag ggatcgatga cgtggcccat
     4621 gcggtcgccc cagaacatgt ccaccggcgc catgtctccc ttcgcgccgg ccgtgaccgc
     4681 gcgctggtag gcggcgtcgc agtcttcgaa gtagagatag aaggagaccg gcgacccgcc
     4741 ctgcgtctcc gccgagcgcg agcccatcac ctcgtcgttc atcatgacga tcgagtttcc
     4801 gatcttgatc tcggcgtgca tcacctttcc gtccggtccg cgcatgattc cgcgttcctc
     4861 ggcgccgaac gcgcgcttgt agaactcgat cgccttcgcg gtgtcgcgca gcgtgatcga
     4921 cggggtgacg gtggtgtagc cttcgggaat ccgtttgacg ttggccatgg gagtcgagcc
     4981 tccgtaacga tgtgggtccg gccggggcga tgctttgaag cgaaggggaa agtataggac
     5041 acgccgctcg gggagccgca acgggtgatt cgcgacttcc tgctgggtcc gccggcgcgc
     5101 gcgcttcgag gagccgtccg ggacggcatc ctcgaagccc cggtcgaata cggactgttt
     5161 ctgctcggct gggcgctctg gctgctggac gccctgatcc ggattccggt cctcgcgcgg
     5221 gagtggagcg cgggccctcg gccccgcgtc gtacccgtcc gcgtcgtccc tctccagcgt
     5281 tcccgtgcgg atccggacac tgcctcggca cgggcccgag ccgttctgac ccaggcaggc
     5341 gcgctcctcg atccgcatgg cgtccggctg aacgtccaga gcgtgaatcc ggtaccgctg
     5401 cccgatgacg tcccgccgcc ggagtgcgga ctccgggcgc tcacggggcg cttcttctcc
     5461 tgggcctcgt cgcgggcggc gggcgcgccc tgcctgaccg tctacttcgt ggaggacctg
     5521 gggccgctcg cgggctgcgc cgtgcccggc gccgattgga tcgtcgcgga gctcggcacc
     5581 gacgggacca cggtggtgca cgaactggga cacctggcgg atctctggag gcaccacccc
     5641 gaccccgaca gcgtcatgac cgaccggccc gggggaaccc acgaccggat ctcgcggtgg
     5701 caggcggccc tgatccgcac ctcgcgcttc gcccttccgg tcagcccccg tcggccgaag
     5761 cgaggcggat cgtgatgggc gtggggtagt ccatcgggcc gatcggcgtg tcgatgcgcg
     5821 gcgtgagctg gacctcgacg tcgtgccggc cgccggcctg gccggcgagg gcggcggcgg
     5881 tctcgaacag cgtccgcgcg tcgccgctga agaagcggta gaggttgaat tcgatcgcga
     5941 tcggaacgtc caccggctgg cccgggggaa agcgcagcgg ctggtccaga cggccgctca
     6001 ggacctcgcg gtcgtccacg cggtaagacc aggcgagcgc gatcatccgc gccgcgacct
     6061 ggttcgactg gggattgcgc ccttccaggt ggagggtgaa gccgaagggg acgtccttgg
     6121 aggcaacccc gagaccgagg cgcgcgacct gcgccgcggt caggctgcga taggtgggaa
     6181 gcgggtcgag ccggatccgg gccacctcgg cgtccgagac ccggtccagg cgaaattcca
     6241 cccggcgcag cgcggccgct tcacgcagcg tcgcgcatcc gaccatcagg ctcaggacgc
     6301 ccaggatgag ataccggatc cgacctcccg cgtcagcgcg ctcctgccgt cgtcattccg
     6361 ggccggctca tgtcgggccg tcccaccgtg ccgccttgat cgaagcgatc ggagcccgcg
     6421 accttcacga tcttgttgta caccccgagc aggaggatcg aagggaccca ctgcccgaag
     6481 aagaggctct tgtgcttgct tccggccacc tgcatcgcca gggaagcgcc gatcgccccg
     6541 acggccgcga tcaggaacca gtccgacggc atcttggacg tctgcttctc gatggccttg
     6601 gccacgccgc cttccgtgcg ctcgggccgg ttcgtcacct tgtctaccgc gtcgctcacg
     6661 ttcaccatgg aacacctccc gtgatgttgg tcttgccgct atcggcaatc ccgttcacgg
     6721 agtgcaagga tggggcccgt gacaaagcgc cgcgcgcctc gttccgattc cacgcggaag
     6781 tggcggttcg acccccacat gtaccagcgc tgcggaccgg ggggcaaggc tttcgcgcgc
     6841 ggacttgaaa cccaagaggg cgccgtggca agaattgcgc gaagttacga gtcgggaccc
     6901 ggcatcacga tccagaggat gatgtacacg agcgtccccg gaaaagcgta gctcacgatc
     6961 gagacgacga cgtagagcac ccgcaccacc gtcacgtccc agcccagcca ttccgcgatc
     7021 ccgccgcaca cgccggcgat catccgattt cgcgagcgac gaagcctcct catggcgtcc
     7081 gccatcatca tcgtcctcca ggggcatgcc cgccggcgtc tcccggcgtg cttgtggatc
     7141 cgtcggtgcc gaagtcgacg aatatctggt agatccgcgg ctccagggcc cgcatgtcgc
     7201 ggttgtccga ggggaattcg tggtcgccct gaacgtgacg atagggcgcg ccggcgcgat
     7261 cctcgatccg cggttccagg tagagccgta ctccgggcag cagcgagtcc agggcgccac
     7321 cttcccagga cgtcacggcc ccggcatagt cccatgcgaa tccggagagc cggaaggggc
     7381 ggccattcgc gctctcgaga tcggccaggc t
//
LOCUS       DASSLZ010000093         4427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_414189, whole genome shotgun sequence.
ACCESSION   DASSLZ010000093 DASSLZ010000000
VERSION     DASSLZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414189"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFM00_03505"
     CDS             <1..823
                     /locus_tag="VFM00_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497341.1"
                     /translation="RLPWALSGPQSYWPGFLAVALALAGVFASWRECYRPAGIPAALA
                     GTGFVLSLGPILHVAGRAIPIPLPYAVLYYVVPGFSGMRAPGRFAVLVALGFAVLAGI
                     GYERCVVFLRGPARRFVGIALSLLIVAGTLDRSIPLLTLPNARTMAPIYRRIAALPGQ
                     EPILEIPVPGTEADENVVDALRQYTVLYHGKPRLDGVSGFVTPRYREFRRVIQSFPDP
                     DALAAARSLGARWILVHFGDYTPAQRAGLAQRVASERLLEPAAREQDDVLYRLGP"
     gene            1019..2044
                     /locus_tag="VFM00_03510"
     CDS             1019..2044
                     /locus_tag="VFM00_03510"
                     /inference="COORDINATES: protein motif:HMM:NF041940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HET7497342.1"
                     /translation="MARHLVTVLVIAWGFWATGCGRNPSGPAADQGADRAAILRAVAA
                     APELAVDFSDDAGELLGTAEPASPIAVQGPLLAPADTGITANAPIHWGRRRTPSDRPP
                     QRIVDFILPPDSGRAVVRVTVRFDGWLYVDRTDDGLRNPGKKPVRDQAVRTALFRKVW
                     FHADSSSADSVFGWRLVALSPVEFSMIDPARQTVAIHSVTVTTPAGATTTTDPSAFLS
                     LRDGGPAVPSTVFGQTVKVEAAVTNTDRSYRPSEFVYLHVPINMRPFPGPFDRVRIRM
                     RDDGSNGDTKAGDGIYTAQWTVQDVGRHHLAVDILSARCLQTESGDDYNSTTWGIPYA
                     SYPAMAQ"
     gene            2310..3896
                     /locus_tag="VFM00_03515"
     CDS             2310..3896
                     /locus_tag="VFM00_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497343.1"
                     /translation="MTSPATPKGRKPERRPAANVNPLPLRAALLWGLAALFVAILFSA
                     ATYRSIAPSSDLWDYSQEARQIARGQGFTSLYTYPTHLGPNDRAPFPVRWRMPLYAAR
                     GAMMLNVGASLPLGYFIVVAQSHAALVALVFLLGAHFASRRAGHVAAATALACPLFLD
                     AYSPGMSQVPVAALSLLVWLLLLRWRGATTAVLAAIVAAAAWYLRGESLLMAPLWAWV
                     AGRGRRRRGIVFAVIYVLLCAPWPVYLAQTTGHASSIQGNPMLLYTPEYPGYSSTRSY
                     GVAMPAAVPYVLAHPMTFAARWIKDALGFGLDLLGGLGPLAIGLAMAGFLLREVHDRL
                     APFRPTVPFLIAIALQVAAFSALERSPRFLVPVAPLACVMIGIAAAPALDRLCGRRAL
                     EALFALLIAERALTVAFQTREAPRHFPPLPPATASALNAAATAGAWPRDRLILSDIPD
                     WVAWHADRAALLLPLARDLARVERDHPVAAIFLSPDARARNAADGDTAWVGVIDRNAP
                     VAGFAGPAFLPGGARLYVRP"
     gene            3893..>4427
                     /locus_tag="VFM00_03520"
     CDS             3893..>4427
                     /locus_tag="VFM00_03520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET7497344.1"
                     /translation="MSRVWRVARAAVALVALAAAGAYAWSGAGKPWDFETYYFAGAAL
                     RAGLNPYSLEDLTRLAHRTVTLPFLYPPATLALFAPMSLMPRTVAEGVWLGFNVFLVA
                     CLAWLWRRQFLRGGDPFRLLVVLALGFDLSAVSGLCTGNVALLEIAILWIALAAYGRG
                     RDALAGYLIALASIFKLL"
BASE COUNT          535 a         1606 c         1481 g          805 t
ORIGIN      
        1 tcgtcttccc tgggctctgt ccggccccca gtcgtactgg cccggattcc tggccgtcgc
       61 cctcgcattg gcgggcgttt tcgcatcgtg gcgggagtgc tatcgcccgg ccggcattcc
      121 cgcagcgctc gccggaaccg gattcgtgct gtcgctcggg ccgattcttc acgtggccgg
      181 acgggcgatt ccgattccgc ttccgtatgc ggttctctac tacgtcgtgc cgggcttctc
      241 cggtatgcgg gctccagggc gcttcgcggt gctggtagcg ctcggctttg ccgttcttgc
      301 ggggatcgga tatgagcggt gtgtcgtgtt ccttcgagga cccgcccgcc gcttcgttgg
      361 catcgcgctc tcgctcctga tcgtggccgg gaccctcgat cgctcgatcc cgttactaac
      421 cctgccgaac gcccgcacca tggcccctat ctaccggcgt atcgctgcgc ttcccgggca
      481 ggagcccatc ttggagattc cggtgcctgg gaccgaggcg gacgagaacg tggtcgatgc
      541 gctccgtcag tacacggttc tctaccacgg aaagccgcgc ctggacggcg tgagcgggtt
      601 cgtgacgccc cgataccgcg agttccgccg cgtgatccaa tcctttccgg atcccgatgc
      661 gctcgcggcg gctcgatcgc tcggggcgcg ctggatcctg gttcatttcg gtgactacac
      721 tcctgcccag cgcgcgggtc tcgcgcaacg tgtcgcctcc gagcgtctcc tcgaacccgc
      781 ggcccgggaa caagacgacg tactgtaccg tctggggcct tgagacccgg ggggcccgcc
      841 ccaatccggg aaccatgagc cgtccgtggg gtccatctct acgaagggct ctcgccggtc
      901 ccccctccgg cgaaaaatat cgcccgctgc cggtcccccc tccggcgaat ggcacacccg
      961 ctgcaaggca ggatcacgcc cggcgggttt gccgcttttt tcccggggag accgcatcat
     1021 ggctcgacac ctcgtcaccg tactcgtcat cgcgtggggc ttctgggcga ccggctgcgg
     1081 gcggaatccg tccggtccgg ccgccgatca gggcgcggac cgtgccgcca ttcttcgcgc
     1141 cgtggccgcc gcgccggagc tggccgtcga tttctcggac gacgccggag agctgctcgg
     1201 caccgcggag ccggcctcgc cgatcgcggt tcaggggccg ctgctcgcgc ccgccgacac
     1261 cgggatcacg gcgaatgcgc ccattcactg gggccgccgg aggacgccaa gcgaccgtcc
     1321 gccccagcgc atcgtcgatt tcatcctgcc gcccgacagc gggcgcgcgg tcgtgcgcgt
     1381 gacggtgcgc ttcgacggat ggctgtacgt ggaccgcacc gacgacggac tgcgcaatcc
     1441 ggggaagaag ccggtgcgcg atcaggccgt gcgcaccgcg ctgttccgca aggtctggtt
     1501 ccacgccgat tcgtcctcgg ccgattccgt gttcggatgg cggctcgtgg cgctctcacc
     1561 ggtcgaattc tcgatgatcg atcccgcgag gcagacggtc gcgatccact ccgtcaccgt
     1621 gacgacgccc gcgggggcga ccaccaccac ggatccctct gcgttcctct cgctgcgcga
     1681 cggcggcccg gccgtgccgt ctacggtctt cggacagacg gtcaaggtgg aggccgcggt
     1741 cacgaacacc gacaggagct accgcccctc ggagttcgtc tacctgcacg tgccgatcaa
     1801 catgcgcccc ttcccggggc cgttcgatcg cgtccggatc cggatgaggg atgacggttc
     1861 gaacggcgat accaaggccg gcgacggcat ctacacggcc caatggaccg tgcaggacgt
     1921 cgggaggcac cacctggcgg tcgacatact cagcgcccgg tgcctccaga ccgagtccgg
     1981 ggacgactac aattcgacta cgtggggtat cccatacgcc tcgtatcctg ccatggcaca
     2041 atgacttggc tggggcgaat tgatggaaac tttttcggtg ctgaggtggt ctaaggaggt
     2101 agagagtccg gcgatctaag cgtctcggtc ctaaagggtt acccagccca cctcctgctg
     2161 gggcccgctg cgtcggacgg ggctctctgg aaccgatagg tccgcggtac cgcaagaccg
     2221 cggccgaaaa gcaaggctcc ccgccgtcgg atgcgcggcg gggagttatt ttttgccccc
     2281 cttcgaagcc cgcctctatt ctccggctcg tgacctcccc agcgactccc aagggccgca
     2341 agccggagcg ccgccccgcg gcgaacgtca acccgcttcc gctgcgcgcg gcactcctct
     2401 gggggctcgc ggccctcttc gtcgcgatcc tcttcagcgc ggccacgtac cgctcgatcg
     2461 cgccctccag cgatctctgg gattactcgc aggaggcgcg gcagatcgcc cgcggtcagg
     2521 gcttcacgtc gctctacacg tatcccaccc atctcggccc gaacgatcgc gcgccgttcc
     2581 cggtgcgctg gcggatgccg ctctacgcgg cgcgcggcgc gatgatgctg aacgtcgggg
     2641 cctcgctccc gctcggatac tttatcgtgg tcgcgcagtc gcatgccgcg ctggtcgcgc
     2701 tggtgttcct gctcggggcg catttcgcga gccggcgcgc ggggcacgtc gcggccgcga
     2761 cggcgctcgc ctgcccgctc ttcctggacg catacagccc cggcatgagc caggtgccgg
     2821 tcgcggcgct ctcgctcctg gtctggctgc tcctcctgcg atggcgcggc gcgacgaccg
     2881 ccgtgctggc cgcgatcgtc gcggccgcgg cgtggtatct gcgcggggaa tcgctgttga
     2941 tggcgccgct ctgggcgtgg gtcgcggggc gcggccgccg gcggagggga attgttttcg
     3001 ccgtcatcta cgtgctcctc tgcgcgccct ggccggtcta cctggcccag accacggggc
     3061 acgcctcgtc gatccaggga aacccgatgc tcctctacac gccggagtac cccgggtatt
     3121 cgtcgactcg aagctacggc gtggcgatgc cggcggcggt tccgtacgtg ctggcgcatc
     3181 ccatgacgtt cgccgcgcgg tggatcaagg acgcgctcgg cttcggactg gatctcctgg
     3241 gcgggctcgg gccgctcgcg atcggcctcg cgatggcggg gttcctgctg cgggaggtgc
     3301 acgaccgcct ggcgcccttc cgccccacgg tgccgttcct gatcgcgatc gcgctccagg
     3361 tcgcggcgtt ctccgccttg gagcggagtc ctcgtttcct ggtacccgtg gcgccgctcg
     3421 cgtgcgtgat gatcggcatc gccgccgcac ccgcgctgga ccgcctgtgc ggccgccgcg
     3481 cgctcgaggc gctcttcgcg ctcctgatcg ccgagcgcgc gctcaccgtg gcgttccaga
     3541 cccgcgaggc gccgcggcac ttcccgcccc tccctccggc gaccgcctcg gcattgaacg
     3601 ccgcggccac ggcgggagcc tggccgcgcg accggctcat cctctccgac atcccggact
     3661 gggtcgcctg gcacgcggac cgcgcagcgc tcctccttcc gctcgcgcgg gacctcgcgc
     3721 gcgtggagcg ggaccatccg gtcgccgcga tcttcctctc gcccgacgcc cgcgcgcgga
     3781 acgccgccga cggcgatacg gcgtgggtgg gcgtgatcga ccggaacgcg cccgtcgcgg
     3841 gcttcgccgg gccggcattc cttccggggg gggcccgcct gtacgtgcgg ccgtgagccg
     3901 ggtgtggcgc gtcgcccgtg ccgccgtcgc gttggtcgcg ctggccgccg ccggcgccta
     3961 tgcgtggagc ggggccggga agccctggga cttcgagacc tactacttcg ccggagccgc
     4021 ccttcgcgcg gggctgaatc cctactcgct cgaggacctc acgcggctcg cacaccgcac
     4081 cgtgacgctg ccgttcctgt atccgcccgc cacgctcgcg ttgttcgcgc ccatgagtct
     4141 tatgccacga actgtcgcag agggagtctg gctcgggttc aacgtcttcc tcgtcgcgtg
     4201 cctggcctgg ctctggcgtc gtcagttcct gcgcgggggc gatcccttcc gcctgctcgt
     4261 ggttctcgcg ctggggttcg atctctccgc ggtgtcggga ctgtgcacgg gaaacgtggc
     4321 gctgctggag atcgcgatcc tctggatcgc tctggctgcc tatgggcggg gcagggatgc
     4381 gctcgcgggc tatctcatcg ccctggcttc gatcttcaag ctcctgc
//
LOCUS       DASSLZ010000094         4471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_287941, whole genome shotgun sequence.
ACCESSION   DASSLZ010000094 DASSLZ010000000
VERSION     DASSLZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287941"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1951
                     /locus_tag="VFM00_03525"
     CDS             107..1951
                     /locus_tag="VFM00_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET7497345.1"
                     /translation="MNELDEIAAHPRPQRRRPWWRSLDGPWEFALEATAVWEMPQDVR
                     WSGTIEVPFAPEAPASGIGDESFHEACWYRRTLEVPRLDPGERLLLHFNAVDYAASVW
                     ANSCRVAYHEGGYTPFVADLTEFAGRSPVTIVVRAHDDPHDLAKPRGKQDWELKPHSI
                     WYPRTSGIWQSVWLERVPETSIDSIRWTPNLERWEIGLEARIGGQAREDLRLRVEMRA
                     NDLLIADDMYAVVAGEVNRKIGFSDPGIDDYRNALLWSPHSPTLIDARLQLIDATGRV
                     IDRVDSYTALRSVTTEGRTVLLNGRPYPLQLVLDQGYWPESGLTAPSVDALRKDVELA
                     KAMGFNGVRKHQKIEDERYYFWADRLGLLVWVEMPSAYRFTSRTIERVTREWLEVLRR
                     DYSHPCVMAWVPFNESWGVPNLPEVPSQRHFVQSLYSLTKTVDPTRPVVGNDGWESSA
                     TDIIGIHDYDDQPERIAARYRSDEELPTLFQRHRPGGRALALAGHPHEGLPIMLSEFG
                     GIAFRAKGDPYWGYSCATAPEDLAARYKELLDVVRASPSLAGFCYTQFADTYQEANGL
                     LDAERIPKIPIELIADATRGPRVPRHQAEQQKRVERGAPGSQHDAAAP"
     gene            1948..3069
                     /gene="galT"
                     /locus_tag="VFM00_03530"
     CDS             1948..3069
                     /gene="galT"
                     /locus_tag="VFM00_03530"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET7497346.1"
                     /translation="MSVPAMPAATIRRRLVKPDGRSVWLYASEPLPEGIAAPEPRGGP
                     VSAAPELRWHPFRAEWVDYAANRQERTFLPPAGYDPLAPTRPGEEPTELPAGPWEVAV
                     FENRFPSLAAGAPDPPRTLVPTRPGHGACEVVVYTQAAEGGLGDLDLTRVEMLIDVWA
                     DRTRELGARADVEYVMPFENRGVEVGATLQHPHGQIYAYPFVPPIPARELEEERRHRA
                     RAGRGLLETHLEAELADGRRVLFDEGGVVAFVPVFARYPYEVWIAPRAPVPGLPDVDA
                     RLRAAFARALRTVVRGYDALRGRPFPYVMVFHQAPTDGAPHPEAHLHVEFYPPYRTSE
                     RLKYLAGTEIGAGMFTNDALPEAKASELQSALAAAKERP"
     gene            3066..4205
                     /gene="galK"
                     /locus_tag="VFM00_03535"
     CDS             3066..4205
                     /gene="galK"
                     /locus_tag="VFM00_03535"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET7497347.1"
                     /translation="MTSEGRALVNGFERRFGREAEITARAPGRVNLIGEHIDYNGGSV
                     LPTVIPQCATVELAAREDRTVRLSSVEAGGAVERYGLGEETPRGTWIDYAQGATHLLA
                     VEGFAFGGVDCCVRSDVPIGSGLSSSAALTVALLRALRERFQLALDDVALARLAQRAE
                     NEFVGARVGVMDPMAASLGREGQALLLRTKDLVYRYVPIPAAVEIVVVDSGQEHQNAG
                     GAYNRRRAECEEAARRLGEALLCDLPVSAWGRIASLPPPLDRRARHVVTENERVHAAV
                     TALEGSRLEELGALLDASHRSLRDDYEVSTPEVDALVTALRSREGVLGARLTGGGFGG
                     AVVAMARAGFGEAAAREAAFEYGRATGLCPTVVMPPGILAAAERE"
     gene            4212..>4471
                     /locus_tag="VFM00_03540"
     CDS             4212..>4471
                     /locus_tag="VFM00_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019008469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HET7497348.1"
                     /translation="MEGGIAASYFMGGFECSTHRLRTGRRLDLAASTRHEEFASEDYA
                     RLKRLGMFTARDGIRWHLIEAVPGRYDFDGARRMVRAALQEGV"
BASE COUNT          632 a         1585 c         1638 g          616 t
ORIGIN      
        1 gccgcgctga aggcccgcga gcggcgcgag gagcgccggt cgctggcgcg acgcccgggg
       61 cggccggcgg cgcggaccga gcggacaccg atgacgctct gagcgcgtga acgagctgga
      121 cgagatcgcg gcgcatccac gcccgcagcg gaggcgtccg tggtggcgct cgctcgacgg
      181 cccgtgggag ttcgcgctcg aggcgacggc ggtctgggag atgccgcagg acgtccgctg
      241 gagcggcacg atcgaggtgc cgttcgcgcc ggaggctccg gccagtggaa tcggcgacga
      301 gagcttccac gaagcctgct ggtaccgccg cacgctggag gttccccggc tcgatccggg
      361 cgagcgcctc ctcctccact tcaacgccgt ggactacgcc gcctccgtct gggcgaacag
      421 ctgccgcgtc gcctaccacg aggggggcta cacccccttc gtcgccgacc tcaccgagtt
      481 cgccggccgc tctcccgtca ccatcgtggt tcgcgcccat gacgatccgc acgacctggc
      541 caagccgcgc ggcaagcagg actgggagct caagccccac tccatctggt acccgcgcac
      601 gtcgggcatc tggcagagcg tgtggctgga gcgcgtgccg gaaacctcga tcgattcgat
      661 ccgctggacc ccgaacctcg agcgctggga gatcggcctc gaggcgcgga tcggggggca
      721 ggcgcgcgag gacctgcgcc tgcgcgtgga gatgcgcgcg aacgacctgc tgatcgccga
      781 cgacatgtac gcggtcgtcg cgggcgaggt gaaccggaag atcggattct ccgatccggg
      841 catcgacgac taccggaacg cactcctctg gagcccccac tcgccgaccc tgatcgacgc
      901 gcggctccag ctgatcgacg cgacgggacg ggtgatcgac cgcgtggaca gctatacggc
      961 gctgcgatcg gtcaccaccg aggggcgaac ggtgctcctg aacggccgcc cctatccgct
     1021 ccagctggtt ctggaccagg gctactggcc cgagtccggc ctcacggcgc cgagcgtgga
     1081 tgcgctccgg aaggacgtcg agctggcgaa ggcgatgggc ttcaacggcg tccgcaagca
     1141 ccagaagatc gaggacgagc gctactactt ctgggccgac cgcctgggac tgctggtctg
     1201 ggtggagatg ccgagcgcct atcgcttcac gagccgcacg atcgaacggg tgacgcgcga
     1261 atggctggag gtgctccggc gcgattacag ccacccctgc gtcatggcct gggtgccgtt
     1321 caacgagtcg tggggcgtgc ccaatctgcc ggaggtgccg tcgcagcgcc acttcgtcca
     1381 gtcgctctac agcctgacga agacggtgga cccgacgcgt ccggtcgtcg gcaacgacgg
     1441 ctgggagagc agcgccaccg acatcatcgg catccacgac tacgacgacc agcccgagag
     1501 gatcgcggcg cgctaccgga gcgacgagga gcttccgacc ctcttccagc gccatcgccc
     1561 gggagggcgc gccctggcgc tggccgggca tccgcacgag gggctgccga tcatgctgag
     1621 cgagttcggc ggcatcgcct tccgggcgaa gggggaccca tactggggct actcgtgcgc
     1681 caccgctccc gaggacctgg cggcgcgcta caaggagctg ctcgacgtcg tgcgcgcctc
     1741 gccgtcgctc gccggcttct gctacacgca gttcgcggac acctaccagg aggccaacgg
     1801 cctcctggac gccgaacgca tccccaagat tccgatcgag ctcatcgccg acgccacgcg
     1861 cggaccgcgc gtgccgcgtc accaggcgga gcagcagaag cgcgtggagc ggggagcgcc
     1921 gggcagccag cacgacgcgg cggcgccgtg agcgttccgg ccatgcccgc cgccacgatc
     1981 cgccgccgcc tggtgaagcc cgacggccgg tccgtgtggc tctacgcgtc cgagccgctc
     2041 cccgaaggga tcgcggcacc cgagccgcgc gggggacccg tctcggcggc gccggagctc
     2101 cggtggcatc cgttccgggc cgagtgggtg gactacgccg cgaaccgcca ggagcgcacg
     2161 ttcctgcctc cggcaggcta cgatccgctc gccccgaccc gccccgggga agagccgacc
     2221 gagctgcccg caggcccgtg ggaggtcgcg gttttcgaaa atcgctttcc gtcgctcgcg
     2281 gccggcgcgc ccgaccctcc gcgcaccctg gtgccgacga ggccgggaca cggcgcctgt
     2341 gaggtggtgg tctacacgca ggccgcggag gggggactgg gggatctgga tctgacgcgg
     2401 gtcgagatgc tgatcgacgt gtgggccgat cgcacgcgcg agttgggggc tcgggcggac
     2461 gtcgagtacg tcatgccgtt cgagaaccgc ggcgtcgaag tcggggcgac gctgcagcac
     2521 ccgcacggac agatctatgc ctaccccttc gtgcccccga tccccgcccg cgagctcgag
     2581 gaggagcggc ggcatcgcgc gcgcgccggc cggggactcc tcgagactca tctcgaggcg
     2641 gagctggccg acggccggcg cgtcctcttc gacgaaggcg gcgtcgtggc gttcgtgccg
     2701 gtgttcgccc gctatcccta cgaagtctgg atcgcgccgc gcgcgccggt gccggggctg
     2761 cccgacgtcg acgcgcggct ccgtgccgcg ttcgcgcgcg cgctgcgcac cgtggtgcgc
     2821 ggctacgacg cgctccgcgg gcgcccgttc ccctacgtca tggtgttcca ccaggcgccc
     2881 acggacggcg cgccacaccc cgaggcgcac cttcacgtgg agttctatcc cccgtatcgc
     2941 acgagcgagc ggctcaaata tctcgcgggg accgagatcg gcgccggcat gttcacgaac
     3001 gacgcgctcc ccgaggcgaa ggcctccgag ctccagtccg cgctggccgc ggccaaggaa
     3061 cggccgtgac gagcgagggg cgggcactcg tgaacggatt cgagcggcgt tttggccgcg
     3121 aggccgagat cacggcgcgc gcgcccgggc gcgtgaacct gatcggcgag cacatcgact
     3181 acaacggcgg ctcggtgctc ccgacggtga ttccccagtg cgccacggtc gagctcgccg
     3241 cgcgggagga ccggaccgtc cggctttcga gcgtggaggc gggcggcgcg gtcgagcggt
     3301 atgggcttgg ggaggagacg ccgcgaggga cctggatcga ctacgcgcag ggagcgaccc
     3361 acctgctggc ggtcgaagga ttcgcgttcg gcggcgtgga ctgctgcgtc cgctccgacg
     3421 tgccgatcgg gagcggcctc tcctcgagcg ccgcgctcac ggtggccctc ctccgcgcgc
     3481 tccgcgagcg gttccagctg gcgctggacg acgtcgcgct cgcgcggctc gcgcagcggg
     3541 cggagaacga gttcgtcggc gcgcgggtcg gtgtcatgga cccgatggcc gcgagcctcg
     3601 gccgggaggg gcaggcgctc ctccttcgca cgaaggatct cgtctaccgc tacgtgccga
     3661 tccccgccgc ggtcgagatc gtcgtcgtgg actcggggca ggagcaccag aatgcgggcg
     3721 gcgcctacaa tcgccgccgc gccgagtgcg aggaggcggc ccgccgcctg ggcgaagcgc
     3781 ttctctgcga cctgccggtc tcggcgtggg ggcggatcgc gtcgctccct ccgccgctcg
     3841 accgccgcgc gcgtcacgtc gtgaccgaga acgagcgggt gcacgccgcg gtcacggcgc
     3901 tggagggctc gcgcctggaa gagctgggcg cgcttctcga cgcctcccac cgatcgctcc
     3961 gcgacgatta tgaggtctcc acccccgagg tggacgcgct cgtcacggca ctccgctccc
     4021 gcgagggagt gctcggggcg cggctcacgg ggggcggctt cggcggggcc gtggtcgcca
     4081 tggctcgcgc cggtttcggc gaagcggcgg cacgcgaagc cgcgttcgag tacggccggg
     4141 ccacggggct ttgcccgacc gtcgtgatgc cgccgggtat tctcgcggcg gcggagcggg
     4201 agtagacggc ggtggaaggg gggatcgcgg ccagctattt catgggcggc ttcgagtgct
     4261 cgacccatcg gctgcgaacc ggccgccgcc tcgacctcgc cgcgtccacc cgccacgagg
     4321 aattcgcctc cgaggattac gcgcggctca agcgactggg gatgttcacc gcgcgcgacg
     4381 ggatccgctg gcacctcatc gaggccgtgc ccggacgcta cgatttcgac ggcgcccgcc
     4441 ggatggtgcg cgccgcgctc caggagggcg t
//
LOCUS       DASSLZ010000095         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1301771, whole genome shotgun sequence.
ACCESSION   DASSLZ010000095 DASSLZ010000000
VERSION     DASSLZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301771"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..758
                     /locus_tag="VFM00_03545"
     CDS             99..758
                     /locus_tag="VFM00_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497349.1"
                     /translation="MACAAWAGGLPTGSTAKTPSSSKAASRSKTSPKLISGGQILCPL
                     TPFSFERDIGLVEGERKDSLWLMIADSSLAVGDSLTLISDEPNPDADTYVAIIGAAVK
                     EPLSNIPRELYGRAVPEPGDRFYRLATPAGALDCCIFGYALRAPRSAFRKAGGRAEAD
                     LDGDGTTEVFQSCGSGDFLNPTVWSGGALTGTRRWTRAYHTDYAVETNCPRSLDSLGT
                     K"
     gene            1117..1989
                     /locus_tag="VFM00_03550"
     CDS             1117..1989
                     /locus_tag="VFM00_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7497350.1"
                     /translation="MTTERPTSALSGAVGLIVFVFLVAVAIMTIVHGIGEQRPLFIVG
                     VGMPLLLLAALAAAGLFTVQPNQAVVLILFGTYVGTVRHSGWWWTNPFNTRKRISLRV
                     RSLNGQAIKVNDAMGNPVEIAAVVVWRVRDSAMATFDVEDFEAFVAVQSETAVRHLAT
                     EYPYDTEGPDSISLRGSIDKVSDFLRSELQQRLQTAGVEVIEARLSHLAYAPEIAGAM
                     LQRQQAAAIIAARQRIVDGAVGMVEMALARLAANNVIDLDEERKAAMVSNLLVVLCGE
                     RAATPVLNAGTLHQ"
     gene            1997..2182
                     /locus_tag="VFM00_03555"
     CDS             1997..2182
                     /locus_tag="VFM00_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497351.1"
                     /translation="MAERKAFLLRIDPALWEALEKWAADELRSVNGQIEYLLARAVDA
                     AGRAPRSAGKRGPKKES"
BASE COUNT          375 a          719 c          743 g          364 t
ORIGIN      
        1 caccggctca agtacaagaa gctggtgcga tgaggcgcgc ggtcgcgttt cgtgcgaccg
       61 cgccgctggc tctcgtggcg tgcgcggctc tgggcttgat ggcgtgcgcg gcgtgggctg
      121 gtggcttgcc gacaggctcc acggcgaaga cgccatcctc ttcgaaggca gcctctcgtt
      181 ccaagacctc accgaagctc atctcgggcg gccagatcct gtgccccttg accccattca
      241 gtttcgagcg cgacatcggc ctcgtcgagg gcgaacgcaa ggacagcctc tggctcatga
      301 tcgcggactc gtcgctcgcg gtcggcgatt ccctgacgtt gatctccgat gaacccaacc
      361 ccgacgccga cacctacgtc gcgatcatcg gcgcggcggt gaaggagccg ctttcgaaca
      421 ttccgcggga gctgtacggg cgggcggtcc cggagcccgg ggatcgcttt tatcgactgg
      481 ccacgcccgc cggcgcgctg gactgctgca tcttcgggta cgccttgcgc gccccgcgta
      541 gcgccttccg gaaagcggga ggccgcgccg aagccgatct cgacggcgac ggcacgacgg
      601 aagtgttcca gagctgcggc agcggcgact tcctgaatcc cacggtgtgg tcgggtggcg
      661 cgctcacagg aacgcggcgc tggacccgcg cctatcacac cgactacgcc gttgagacca
      721 actgcccccg aagcctcgat agtctgggaa cgaagtagag agctcgcttc gcgctttcgc
      781 gcgttgggcg gatagctgcc ccgcacaaag cagacaattc aacgatttca catgcttcaa
      841 agcatgtgat tctcgcgaca cctgtcaatg gattcaccgc cgctagcggc ggtaaatttg
      901 ttgccgctcc cttccgagcg aggaaggcca gtgaaatcgg ggccagcggg tgaatgacat
      961 agctcgttca cctaacgtca gccaccgacc gctcttccgc gaaaacgctc cggcacccct
     1021 tgccgccgag ttatcattgt gatatcataa tgacatcaac tgccgcgaac tgtttcgcgg
     1081 cttgcatccg tggatttgaa aggaggccgt agaaccatga ccaccgaacg tcccacctcg
     1141 gccctcagcg gcgccgtggg cttgatcgtc ttcgtcttcc tggtcgcggt ggcgatcatg
     1201 acgatcgtgc acgggatcgg cgagcagcgc ccgctcttca tcgtcggcgt ggggatgccg
     1261 ctcctcctgc tggcggcgct ggccgccgcg gggctcttca ccgtgcagcc gaaccaggcg
     1321 gtggtactga tcctgttcgg cacctacgtc ggcacggtgc ggcacagcgg gtggtggtgg
     1381 accaacccgt tcaacacgag gaagcggatc tcgcttcgcg tgcgcagcct gaacgggcag
     1441 gcgatcaagg tgaacgacgc catgggcaac ccggtggaaa tcgcggcggt cgtggtctgg
     1501 cgcgtgcgcg actcggccat ggcgacgttc gacgtcgagg acttcgaagc gttcgtggcc
     1561 gtgcagagcg agaccgcggt gcgccacctg gcgacggagt atccctacga caccgagggg
     1621 ccggactcca tctcgctccg cggcagcatc gacaaggtct ccgacttcct gcgctccgag
     1681 ctccagcagc gcctgcagac cgcgggcgtc gaggtgatcg aggcgcgcct cagccatctg
     1741 gcctacgcgc cggagatcgc cggcgcgatg ctccagcggc agcaggccgc ggcgatcatc
     1801 gccgcgcgcc agcgtatcgt ggacggcgcg gtcggcatgg tggagatggc gcttgcgcgc
     1861 ctcgccgcga acaacgtcat cgacctcgac gaggagcgga aggccgcgat ggtctcgaac
     1921 ctcctcgtgg tgctgtgcgg cgagcgcgcg gcgacgcccg tgctgaacgc cggcacgctg
     1981 caccaatagg agcggaatgg cggagcggaa agcgttcctg ctgcggatcg atcccgcgct
     2041 ctgggaagcc ctggagaaat gggccgcgga cgagctgcgc agcgtgaacg gccagatcga
     2101 gtacttgctc gcgcgcgccg tggacgccgc gggaagggcg ccgcgctcgg cgggcaagcg
     2161 cggaccgaag aaggagagct agagggcgac gcaggacagc t
//
LOCUS       DASSLZ010000096         8535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1174220, whole genome shotgun sequence.
ACCESSION   DASSLZ010000096 DASSLZ010000000
VERSION     DASSLZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174220"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFM00_03560"
     CDS             complement(<1..227)
                     /locus_tag="VFM00_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497352.1"
                     /translation="MKSTLRTLDSGAGIPAPSGANGAAVYPHSVLADRPGPDFEAIKT
                     RQQAAWASGDYGQVGTRLQIVGELLCEAVDLL"
     gene            417..1439
                     /locus_tag="VFM00_03565"
     CDS             417..1439
                     /locus_tag="VFM00_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7497353.1"
                     /translation="MSGDTLSDLLRAVRFRGAVFYYIEGSPPWVAEAPPAREIIPAIM
                     PGVEHMIEFHGVVKGSCWAAMVGEPPVRLEERDLILFPQGDPHVVSSAPGMRPQSVDP
                     NLYFTPRPPQLPYALSMEGERINTTTLDGGGRDRTTIICGFLGLDAKPFNPLLAALPR
                     VLKVPGSSLGPESWVANFLRAVVLESNARRPGGEAVLERMSEMLFVEALRRYIDSLPA
                     EETGWLAGTRDPAVGRALALLHERPGQVWTIERLGEEAGMSRSSLHERFVHFIGQPPM
                     QYLTQWRMQLASGILRETNAKVLEIALEVGYESEAAFSRAFKRAVGMAPGAWRRSKRS
                     PGPSRT"
     gene            complement(1436..3400)
                     /locus_tag="VFM00_03570"
     CDS             complement(1436..3400)
                     /locus_tag="VFM00_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497354.1"
                     /translation="MIAPFRSLAAVRRACHSFHAALVMALLLGAAPARAQSWIPVGAP
                     GGNVRSLAADPSRPERVYLGTAEGILYRSDDAGTRWTRLDPGFPCRGCSLDEIKVDSD
                     GTVYVGYWEVHGAGGGVARSTDGGESFTLLPGIQGESVRALAIAPSNERVIAAGTLTG
                     VFLSKNGGQSWARISPAGHPDLRNIESLAFDPDNPRVLLAGTWHLSWKTVNGGAAWAA
                     AHDGIIDDSDVMTLTVDRRNPRALFATACSGIYHSEDGGARWTKLPGIPYSSRRTRAF
                     ALGDGLLLAGTTEGLFVSEDDGGTWRRATRKELVVNAVVALPGGGILLGTEGAGVLRS
                     TDRGQTWVTSNTGFSERFVFKVLFDDPGSRIVVAEWGAPHYGGVFVSPTVGGPWQRLG
                     EGMDGRQVLSLAVLGPTLLAGTDDGVFARAPDAPAWVRLPTRIDGQEAHPRVTELIAR
                     TPGTLLAATTKGLMASEDGGRSWKRAERVGDAEVTGLAASPEDPDLVIAATKSGVFRS
                     RDGGATWSRVSDALGAGVHAVAFMPTDDRVLFATTNGGLYRSRDQGATWRRLGGGLPH
                     SDLTGIALDPGGRAVYVSDFTWGGIFQSRDGGSTWKRMTTDGLASDHVWTLSVDPGTP
                     GRLLAAASAGGLHVLSQGSLASRAATRGSD"
     gene            complement(3545..4771)
                     /locus_tag="VFM00_03575"
     CDS             complement(3545..4771)
                     /locus_tag="VFM00_03575"
                     /inference="COORDINATES: protein motif:HMM:NF017546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7497355.1"
                     /translation="MRSTIMALVAVLLVSATPAFAGGEKGQLELGIYGGHIWFDDYGI
                     FRPENKFFPGVRLGYWLSDKVSLEASGQRVKSETDFEILGLENTDVTAEALRLNLLYN
                     LGHGGFRPFLTAGAGNEKFHAEGFGESCDFGWNAGAGFRAFLSPGVALRADGRYVSVK
                     VGDEVDESQGNVEAMLGLSFLFGGHHEHVEEVHTEVANQPPTVTCVVDRAQILPGETA
                     SITVTASDPEGGPVTYEYNSPTGHVAGNGAVATFDFASVAPPSTATITVRVTDDHGNT
                     STCDATVALMEPQRKAEAVSCIAGGFPNNLSRITNVDKACLDDVAQRLSADPRATVVI
                     IGHADSHERSSDIAQRRADAIRDYLVNERHVEVARVTTRSAGSTKMIATGSDRDSQAQ
                     NRRVEVWFVPEGASGP"
     gene            complement(4837..5526)
                     /locus_tag="VFM00_03580"
     CDS             complement(4837..5526)
                     /locus_tag="VFM00_03580"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HET7497356.1"
                     /translation="MLALVVAGCCDKDKPLGVLESGGAGPAVVVLGTTSTYAVLAQDS
                     VVSTGATVITGDIGVSPGTTVTGFPPATVSGTTQAGNATSATALVDLTTAINDVTARS
                     TTVLTLPGSELSGMTLSPGLYDTASPLTLSGTVTLNALGNPDAVFIIRTAADLTTAAG
                     SKVVLTGGAQAKNVIWLVGTTATLGSNSVFQGTILANQSITLLSGARLNGRALSRTGA
                     VTLDAAFIVVP"
     gene            5913..8078
                     /locus_tag="VFM00_03585"
     CDS             5913..8078
                     /locus_tag="VFM00_03585"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HET7497357.1"
                     /translation="MGFDVVARASRLAVLVVVSWLVALPAFGGEPPAVEFLSPRPGAA
                     MILPETNIIVRPGGVVTESGSECLRVTGSVSGSHAGTLRLSDDRRTVTLQPDLPFVVS
                     ETVTCRLETGLSTDSRGPIAPGVFSFTIAGPEREALRDQPAPADVDEDLASALASGSP
                     AAGNASSAAPAESLPFDFPAIHAEVPGVPAPGRLFVSDVFFNIRGPRIPSYLMILEND
                     GTPYWYRQIDGVGLDFKMQPDGRLTYFDSAHDAFYAMDARYAVVDSFRSGNGYSTDNH
                     DLLLLPNGHAVLMSADPQIMDLSAIGGYPNAVVIGLIIQELDQEKNVVFQWRSWDHFQ
                     ITDMLFHRIDVPLVDYVHGNSIDVDSQGNFILSSRHMNEVTKISRATGEILWRLGGKN
                     NQFRFVNEPIAFSHQHHARLLPDGHLTMFDNGNFRLPQFSRAVEYAIDETNKTATLVW
                     QYRLTPDVFGVAFGSVQRLGNGNTLIGWGATQPTLTEVDPVGRIVSRLTFDSGVASYR
                     AFRFEWPSFKPASIAFDPSTIALDPATGQDPERGTIRARIAPVDSDFSAAEVIVPSIR
                     LGGSVLADTTSAQADPDGLVVLFPRDGVEPLLAPGTNRLEVTGSLTSGERFRGFGSIE
                     AVGTLARRDSGAPPKLVSAPGRLPVELVTAGVSGTRQTFAVYDIHGRLVRRGQVPAGA
                     ARVSWDGRDRDGRPAASGVYLIRVEDGSPRSAAKVAIVR"
     gene            8286..>8535
                     /locus_tag="VFM00_03590"
     CDS             8286..>8535
                     /locus_tag="VFM00_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497358.1"
                     /translation="MARPVRIVLAFAALALIGAPSARAQQILIDKPVRAGELMCFPDL
                     NDPTAYYYVNDKPHLATDANGKPQFSFLRYVENVRSGAT"
BASE COUNT         1270 a         2922 c         2937 g         1406 t
ORIGIN      
        1 aggaggtcga ccgcctcgca gagcagctcg cccacgatct gcagccgcgt gccgacctgg
       61 ccgtagtctc cggaggccca cgcggcctgc tggcgggtct tgatcgcctc gaagtccggc
      121 cccgggcggt cggccaggac cgagtggggg tagacggcgg cgccgttcgc gccgctcggc
      181 gcggggatgc cggcgccgga gtccagagtc ctcagggtcg atttcatggc ggctctcctt
      241 ctcgtcgtgg ggttccgtcg ttccgcggcc ccggcggggc ggcgcgcaca cgaataggat
      301 gccgcgcggc tctcattgac gtctggctgc gcgtcgggaa cacttgtccg atcgtccaga
      361 acttcacgaa gccttgccgt ccgtccgccg gctcttgccg gccatccggg gagacgatga
      421 gcggggatac cctttccgat ctcctgcgcg ccgtccggtt tcgcggcgcg gtcttctact
      481 acatcgaggg ctcgccgcct tgggtggcgg aagcgccgcc tgcgcgcgag atcattccgg
      541 cgatcatgcc cggcgtggag cacatgatcg agttccacgg cgtcgtgaag ggatcgtgct
      601 gggccgcgat ggtgggggag ccgcccgtcc gcctcgagga gcgcgatctg atcctctttc
      661 cgcaggggga tccgcacgtc gtctccagcg cgcccggcat gcggccgcag tcggtggatc
      721 ccaatctcta cttcacgccg cgcccgcctc agcttccgta cgcgctcagc atggagggag
      781 agcggatcaa caccacgacg ctcgacgggg gcggccgcga ccgcacgacc atcatctgcg
      841 gattcctggg tctcgacgcc aagcccttca atccgctgct cgcggcgctc ccgcgtgtgc
      901 tgaaggttcc ggggagctcg ctcggccccg agagctgggt ggcgaacttc ctgcgggccg
      961 tggtcctgga atcgaacgcg cgccgtcccg gaggcgaggc ggtgctggag cggatgagcg
     1021 agatgctctt cgtcgaggcg ctccgccgct acatcgactc gcttccggcg gaggagaccg
     1081 gctggctggc ggggacgcgc gatcccgcgg tcggccgggc cctggcgctc ctgcacgagc
     1141 gtccaggcca ggtgtggacg atcgaacgtc tcggcgagga ggctgggatg tcgcggtcgt
     1201 cgctccacga gcggttcgtg catttcatcg gccagccgcc gatgcagtat ttgacccagt
     1261 ggcggatgca gctcgcgtcg gggatacttc gcgaaacgaa cgccaaggtg ctggagatcg
     1321 ccctcgaggt gggctacgag agcgaggcag ccttttcgcg ggcgttcaaa cgcgccgtgg
     1381 ggatggctcc gggcgcgtgg cgccggtcga aacgctcgcc gggaccgtcg cgaacctagt
     1441 cggatccgcg cgtcgccgcg cgcgaggcca gcgagccctg cgagagtacg tgcagccctc
     1501 ccgccgaggc cgccgcgagc agccggcccg gcgtgccggg atcgacgctc agcgtccaca
     1561 catggtcgga cgccaatccg tccgtcgtca tccgcttcca ggtcgagccc ccgtccctgc
     1621 tctggaagat gcctccccac gtgaagtcgc tcacgtagac cgcccggccc ccggggtcga
     1681 gggcgatgcc ggtgaggtcg gagtggggca gtccgccgcc gaggcggcgc cacgtcgccc
     1741 cctgatcgcg agagcggtag agcccgccgt tggtcgtcgc gaagaggacg cgatcatcgg
     1801 tgggcatgaa ggccaccgcg tggacccccg cgccgagagc gtcggagacg cgcgaccagg
     1861 tggctccgcc gtcccggctg cggaagacgc cggacttcgt cgcggcgatg acgaggtcgg
     1921 ggtcttcggg tgaagccgca agcccggtga cctccgcgtc gccgacgcgc tcggcgcgct
     1981 tccagctgcg cccgccgtct tcgctggcca tgagtccttt cgtggtcgcg gccagcagcg
     2041 tcccgggcgt ccgcgcgatc agctccgtca cgcgaggatg cgcttcctgg ccgtcgatcc
     2101 gagtgggcag ccgcacccac gccggcgcgt ccggcgcgcg cgcgaagacg ccgtcatccg
     2161 tgccggcgag cagcgtgggc ccgagcacgg ccagggagag aacctggcgt ccatccatgc
     2221 cctcgccgag ccgctgccag ggcccgccca cggtcgggga gacgaacacg ccgccgtagt
     2281 ggggagcgcc ccattccgcg accacgatcc ggctgcccgg gtcatcgaag agcaccttga
     2341 agacgaaccg ctcggagaag cccgtgttgc tggtcaccca ggtctggccg cggtcggtgc
     2401 tgcgcaggac gccggcgccc tcggtcccga ggagaatgcc gccgccgggc agggccacca
     2461 ccgcgttcac gaccagctcc ttgcgggtcg cgcgccgcca ggtgccgccg tcgtcctcgc
     2521 tgacgaagag cccttcggtg gttcccgcga ggagcagccc gtcgcccagg gcgaacgccc
     2581 gggttcgccg gctcgagtaa gggatgccgg gcagcttggt ccaccgcgcg ccgccgtctt
     2641 cggagtgata gatccccgag cacgcggtgg cgaagagcgc gcgggggttg cgccggtcca
     2701 cggtcagggt catcacgtcc gagtcgtcga tgattccatc gtgcgcggcg gcccaggcgg
     2761 cgccgccgtt cacggtcttc caggagagat gccacgttcc ggcgagcagg acgcggggat
     2821 tgtccggatc gaaggccagc gattcgatgt tccgaagatc ggggtgtccc gccggagaga
     2881 tgcgcgccca gctctggccg ccattcttgg agaggaagac gccggtcaag gtcccggccg
     2941 cgatcacgcg ctcgttcgag ggggcgatgg ccagcgcgcg caccgattcg ccctggatcc
     3001 ccgggagcag cgtgaacgat tcgcccccgt cggtgctgcg cgcgacgccg ccgcctgccc
     3061 cgtggacctc ccagtagccc acgtacaccg ttccatccga atccaccttg atctcgtcga
     3121 ggctgcaccc gcggcaggga aatcccgggt cgagccgggt ccaccgggta ccggcgtcgt
     3181 cggatcggta gaggatcccc tcggcggtcc cgaggtagac ccgctcgggg cgactcggat
     3241 cggccgcgag cgaacgcacg ttgccgcccg gggctcctac ggggatccag ctctgggcgc
     3301 gcgccggggc cgctcccaga aggagcgcca tgacgagggc ggcatggaag gaatgacacg
     3361 cgcggcggac cgcggcgagc gaccggaacg gggcaatcat cgtagtccta tatgcttcac
     3421 cgcgaggccg gcccatccgg gcccgcggaa tcgaagtctc ataacgtgta ttgcatcagg
     3481 cgtgccgcgg ggcgaacccg ttcgggcccg ccccgcatgt gcactgcatt tgacgccgga
     3541 tcgattacgg tccgctcgct ccttcgggca cgaaccagac ctcgacgcgg cggttctgcg
     3601 cctgcgaatc cctgtcggaa ccggtcgcga tcatcttggt gctgccggcg gaccgagtcg
     3661 tgacgcgcgc cacttcgacg tgccgctcgt tcaccaggta gtcgcggatc gcgtcggcgc
     3721 gccgctgggc gatgtcggac gaccgctcgt gagagtccgc gtgcccgatg atgaccacgg
     3781 tggcgcgtgg atcagcgctc agccgctgcg ccacgtcgtc caggcacgcc ttgtccacat
     3841 tggtgatccg ggagagattg ttcgggaagc cgcccgcgat gcaggagacc gcttcggctt
     3901 tccgctgcgg ctccatgagc gcgaccgtgg cgtcgcaggt cgacgtgttg ccgtggtcgt
     3961 ccgtgacgcg caccgtgatg gtcgcggtcg agggcggggc cacgctggcg aagtcgaagg
     4021 tggcgacggc gccgtttccg gccacgtgcc cggtcggcga gttgtactcg taggtgaccg
     4081 gtccgccctc gggatcgctc gccgtgacgg tgatgctcgc ggtctcgccg ggaagaatct
     4141 gagcgcggtc gacgacgcag gtcaccgtcg gcggctggtt ggccacctcg gtgtgcacct
     4201 cttcgacgtg ctcgtgatgc ccgccgaaca ggaagctcaa gccgagcatc gcttccacgt
     4261 tgccctgcga ttcgtccact tcgtcgccga ccttcacgct cacgtagcgg ccgtccgcgc
     4321 ggagcgccac gccgggcgag aggaaggcgc ggaagcccgc gccggcgttc caaccgaagt
     4381 cgcacgattc gccgaaccct tcggcgtgaa atttctcgtt gccggcgccc gcggtcagga
     4441 acggccgaaa gcctccgtgc cccaggttgt agagcaggtt gaggcgcagc gcctccgcgg
     4501 tcacgtcggt gttctccagt ccgagaatct cgaagtcggt ctccgacttc acccgctggc
     4561 cggaggcctc caggctcacc ttgtcggaga gccagtagcc caggcggacg ccggggaaga
     4621 acttgttctc gggccggaag attccgtagt cgtcaaacca gatgtgcccg ccgtagatgc
     4681 cgagctccag ctggcccttc tcgcctcccg caaaggccgg agtcgcggac acgaggagca
     4741 cggcgacgag cgccatgatg gtgcttcgca tggaatgtcc tttcctgatg gacgcggggc
     4801 ggtcggtccg cgggcgtccc gtacgatccc cggacctcat ggcacgacga tgaacgctgc
     4861 atccagcgtc accgctccgg tgcgggacag ggcccggccg ttcagccgcg ctccggagag
     4921 gagcgtgatc gactgattcg ccaggatcgt tccctggaag acggaattgg agccgagggt
     4981 cgccgtggtg ccgaccagcc agatcacgtt cttggcctgg gcccctccgg tgaggacgac
     5041 cttgctgccc gcggcggtcg tgagatcggc cgccgtacgg atgatgaaga cggcatcggg
     5101 attgcccagc gcattcagcg tgaccgtgcc cgaaagcgtg agcggggagg ccgtgtcgta
     5161 gagtcctggg gaaagggtca tgccggacag ctcgctgccc ggcagcgtca gcaccgtcgt
     5221 cgaacgcgcc gtcacgtcgt tgatcgccgt cgtcaggtcg acgagcgcgg tcgccgaagt
     5281 ggcgttgcct gcctgggtcg tgccgctcac cgtggcgggt gggaagcccg tcacggtcgt
     5341 gccggggctg acaccgatgt cgccggtgat gaccgtggcg cccgtgctga ccacggagtc
     5401 ctgcgccagg accgcgtacg tcgaggtcgt tccgagaaca actacggccg gacccgcgcc
     5461 gccgctctcg agcactccga gaggcttgtc cttgtcgcag catccagcga ccacgagcgc
     5521 gagcaatggc gccgcgatcc acgaaattcg actctctatg cgcatcattg gattcctcct
     5581 cataccgccg ggccatgact tcgaaacgta gctccgggtt ggaggagcgc cgttggcccg
     5641 gctcaccggg aggggccggc acggatctgc cgccccacag aatccggcat tccacgcagg
     5701 aagagtgccc gcgggcccca tggcgcctga ggggtcggcc taagctgctc cgtctggacg
     5761 agttaagccg agggggtttt ggaggcggcc gggtgtatca tcgatgcctc tttgggaaag
     5821 aggtttcaaa ccccgggcgt cttcttcaac cggcggggca gtacgggcgg cggcggcact
     5881 tgcttgcttg gaccacggaa aggtcgccct tcatgggttt tgatgtcgtc gcacgagcct
     5941 cgaggctcgc ggtcctcgtc gtcgtctcgt ggctggtcgc cctcccggcg ttcggcggcg
     6001 agcctccggc ggtggaattc ctgtcgccgc gtcccggcgc cgccatgatc ctccccgaga
     6061 ccaacatcat cgttcgtccc ggcggcgtcg tgaccgagag cggaagcgaa tgcctccgcg
     6121 tcacgggttc agtttcaggt tcgcacgccg gtacccttcg cctctccgac gaccgccgaa
     6181 ccgtgacgct tcaacccgat ctcccgttcg tcgtctccga gacggtgacc tgccggctcg
     6241 agacggggct ctccaccgat tcgcgcggcc cgattgcgcc cggcgtcttc tccttcacga
     6301 tcgcgggacc cgagcgcgaa gcgctgcggg atcagccggc gcccgccgac gtggatgaag
     6361 acctcgccag cgcccttgca tcgggctcgc ctgcggcggg aaatgcgtcg agcgccgctc
     6421 ccgccgagtc cctgccgttc gatttccccg cgatccatgc cgaggttccg ggtgtgcccg
     6481 cgccggggcg gctcttcgtg agcgacgtct tcttcaacat ccgcgggccg cggatcccgt
     6541 cgtatctcat gattctggag aacgacggca cgccctactg gtaccggcag atcgacggcg
     6601 tgggcctcga cttcaagatg cagcccgacg ggaggctcac ctatttcgac tccgcgcacg
     6661 acgcgttcta cgcgatggat gcccggtacg cggtggtcga cagcttccgc tccggcaacg
     6721 ggtattcgac cgacaaccac gacctgctgc tccttccgaa cggacatgcc gtgctgatga
     6781 gcgcggatcc tcagatcatg gacctgagcg ccatcggcgg atacccgaac gcggtcgtca
     6841 tcggactgat catccaggag ctggaccagg agaagaatgt cgtcttccag tggcggagct
     6901 gggaccactt ccagatcacc gacatgctct tccatcgaat cgatgtgccg ctggtggact
     6961 acgtgcacgg caactcgatc gacgtggact ctcaggggaa cttcatcctc tcctcgcgcc
     7021 acatgaacga ggtgaccaag atcagccggg cgacgggcga gatcctctgg aggctgggcg
     7081 ggaagaacaa ccagttccgg ttcgtgaacg agccgatcgc gttctcccac cagcatcacg
     7141 cgcgcctgct ccccgacgga catctcacga tgttcgacaa cggcaacttc cgcctcccgc
     7201 agttctcgcg cgcggtggag tacgcgatcg acgagacgaa caagaccgcg acgctcgtgt
     7261 ggcagtaccg cctgaccccc gacgtgttcg gcgtcgcgtt cggctcggtg cagcgcctgg
     7321 gcaacggcaa caccctgatc gggtggggcg cgacccagcc gaccctgacc gaggtggacc
     7381 ccgtcggccg gatcgtctcg cgcctgacgt tcgacagcgg cgtggcctcc taccgcgcgt
     7441 tccgcttcga atggccttcg ttcaagcccg cgtcgatcgc gttcgatccc tccacgatcg
     7501 cgctggatcc ggctaccggg caggatccgg agcgcgggac gatccgggcc cggatcgcgc
     7561 ccgtggacag tgatttttcc gcggcggagg tcatcgttcc gagcatccgc ctgggcggct
     7621 ccgtcctggc ggatacgacg agcgcccagg ccgatcccga cggcctggtc gtgctctttc
     7681 cgcgcgatgg ggtcgagccg ttgctcgcgc cgggaacgaa ccgcctcgag gtgaccggat
     7741 cgctgaccag cggagaacgg ttccggggat tcggctcgat cgaagcggtg gggaccctcg
     7801 cgcgccgcga ctcgggcgcg ccgccgaagc tggtatcggc gccggggagg ttgccggtgg
     7861 agctcgtaac ggcaggtgtc tccggaacgc gacagacctt cgccgtctat gacatacatg
     7921 gacgcctggt gcggcgcgga caggttcctg ccggcgccgc gcgggtctcg tgggatggac
     7981 gggatcggga cggccgcccg gccgcgtccg gcgtctacct catccgcgtg gaagacggct
     8041 cgccgcgcag cgccgcgaag gtcgcgatcg tccgttagcg cagcacggga cctctgcgct
     8101 cccagcgggt cctcaccccc acggctcgat gttcggtggg ttgttctcga acgtattccc
     8161 ctttcaagac cattccgaca tccagtaagg ggctgactcc ggctccggcc cgtggtagag
     8221 aagtagctct cgacgtcctt actcccgcgt tccaggcgac actcacgtca cggcggaggt
     8281 cccctatggc acgacccgtt cgcatcgtcc tcgcattcgc cgcgctcgcg ctgatcggcg
     8341 ctccctccgc gcgggcgcag cagatcctga tcgacaagcc ggtgcgcgcg ggagagctga
     8401 tgtgcttccc ggacctgaac gatccgacgg cgtactacta cgtgaacgac aagccgcatt
     8461 tggcgaccga cgccaatgga aagccccagt tctcattctt gaggtacgtg gagaacgtgc
     8521 ggtcgggagc gacgg
//
LOCUS       DASSLZ010000097         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1429040, whole genome shotgun sequence.
ACCESSION   DASSLZ010000097 DASSLZ010000000
VERSION     DASSLZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429040"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="VFM00_03595"
     CDS             complement(<1..169)
                     /locus_tag="VFM00_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7497359.1"
                     /translation="MRLTDLFVRRPVLAMVVSLVVLIAGLQSIRSLSVRQYPKSDIAV
                     VTVTTAYVGAGA"
     gene            complement(169..1299)
                     /locus_tag="VFM00_03600"
     CDS             complement(169..1299)
                     /locus_tag="VFM00_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7497360.1"
                     /translation="MRRRMVFTLAALVVFLTAIGLVKFFQIRAAIAQFSSWQPPPEAV
                     TTTVAREQSWPTTLQAVGSVAAVHGVMVSADLPGIVEQIAFDSGRHVKAGEILVRLDT
                     RQERAQLAAAESQRDLMQLNLDRAKQLLAKGVVAQAEYDRVAAEAKQAEARAGEIRAT
                     IERKRIRAPFSGVLGIRQVNLGQYLEGGAPVVPLQSMDPVYVDFALPQDDVKSLRPGM
                     TVEITADSTTGSAEIAGQINAINSVVDEATRNVQVQATFRNTAGRLRPGMYVEVHAHV
                     GTSGPMIAVPASAVSYAPYGNSVFVVAPQKGPNGKTYSGVEQRFVKLGAGRGDQASVL
                     SGLKPGEVVVTSGVFKLRNGAAIQVNNSIRPSDNPAPRPEDS"
     gene            complement(1313..2863)
                     /locus_tag="VFM00_03605"
     CDS             complement(1313..2863)
                     /locus_tag="VFM00_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7497361.1"
                     /translation="MTRRALELGTITALALGAMLAAMPAPVAGRSLSLNEAIEMSLRR
                     NEGLVIDRESLASAKAAVRRAHGAYDPVLEGQGGWSRSTEPTVLYGSPLGVIAPEVET
                     GDAAVSLRQLLPTGGALSLHAQGSRETVGEGLAFLSPAYRTQVGAQLRQPLLRDRNTD
                     AARLSVRVARAGRAGAAASLRRSVSETVASVERAYWTLTEARLEVGVRQEAVDLAQSQ
                     LDETGHRVDIGEAPKTELAQPRAELERRRGDLFAAREALARAENALKLLILDGADDAL
                     WSEDLAPVETDDPEPAPVDVARALDHAFAQRPELDVAESVIERRRAEASYYQDGIWPR
                     LDAVLRYDRFGLSGLAGSGGTLPPELDGRWDRSFDSLERGGYHAARVGVELQLPILNR
                     EARGNAAIARHAERQAAADAGRIKKQIRAEVLDATAAVETAGQRIEAARSGREAAEVQ
                     LAAERDRYDAGLSTNFLILTRQNDLSRARLDEISALTDYRTARTELARATGQLIEDRG
                     IQLDDSSR"
     gene            complement(2860..3117)
                     /locus_tag="VFM00_03610"
     CDS             complement(2860..3117)
                     /locus_tag="VFM00_03610"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HET7497362.1"
                     /translation="MSQVNVERVIGILATDEALRRRFTHDARAVLTELIQRGMELTAG
                     EQISLAGLDPKVLARFSRYIDDRLQRCDLGHGGSHLKEGVS"
     gene            complement(3446..>3717)
                     /locus_tag="VFM00_03615"
     CDS             complement(3446..>3717)
                     /locus_tag="VFM00_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7497363.1"
                     /translation="AIERAILLCDGGLITREHLPTPPRRSPAPNGNGVDTLALPVDGI
                     DLAAVERGFLERALGQAKGNKTKAARLLGLSRAQLYSRLEKYGVQ"
BASE COUNT          526 a         1314 c         1305 g          572 t
ORIGIN      
        1 cggcgcccgc ccccacgtac gcggtcgtca ccgtgacgac cgcgatgtcg ctcttggggt
       61 actgccgcac gctcagggag cggatcgact ggagtccggc gatcaggacg accaggctga
      121 cgaccatcgc cagcacgggc cgtcgaacga acagatcggt gagtcgcatc agctgtcctc
      181 cggcctcgga gcgggattgt cgctcgggcg gatcgagtta ttgacctgga tcgcggcacc
      241 attccggagc ttgaacacgc cggacgtcac cacgacctct ccgggcttga gcccggagag
      301 caccgaagcc tgatccccgc gcccggcgcc cagcttcacg aagcgctgct cgacgccgga
      361 gtaggtcttg ccgttcgggc ccttctgggg cgccaccacg aatacggagt ttccgtacgg
      421 cgcgtaggag accgccgagg ctgggaccgc gatcatggga ccgctggtgc ccacgtgggc
      481 gtgcacctcg acgtacatcc ccggccgcag ccttccggcg gtattccgga acgtggcctg
      541 cacctggacg tttcgcgtcg cctcgtccac gaccgagttg atcgcgttga tctgccccgc
      601 gatctcggcg ctgccggtcg tgctgtccgc cgtgatctcc acggtcatgc cgggccgaag
      661 cgacttcacg tcgtcctgcg gcagggcgaa gtcgacgtag acggggtcca tcgactggag
      721 cggcacgacg ggcgctcccc cttcgagata ctggccgaga ttcacctggc ggatcccgag
      781 caccccggag aagggggcgc ggatccgctt ccgctcgatc gtggcccgga tctccccggc
      841 gcgcgcctcg gcctgcttcg cctcggcggc gacccggtcg tattcggcct gcgccacgac
      901 gcccttcgcg aggagctgct tcgcccggtc caggttgagc tgcatgagat cccgctgcga
      961 ctcggcggcc gcgagctggg cgcgctcctg ccgcgtgtcc agccggacga ggatctcgcc
     1021 cgccttcacg tgccggccgg agtcgaacgc gatctgctcc acgatcccgg gcaggtcggc
     1081 gctcaccatc accccgtgca ccgccgcgac cgagccgacc gcttgcaggg tggtcggcca
     1141 ggactgctcg cgcgccacgg tcgtggtgac cgcctcgggc ggcggctgcc aggaggagaa
     1201 ctgcgcgatc gcggcacgga tctgaaagaa cttcacgagg ccgatggccg tcaggaagac
     1261 gaccagggcc gccagcgtga acaccattcg ccttcgcatg acgtatccct ccctatcgag
     1321 acgagtcgtc gagctggatg ccgcggtctt cgatcagctg gccggtcgcg cgcgccagct
     1381 cggtgcgcgc cgtccggtag tccgtgaggg ccgagatctc gtcgagccgg gcccgggaca
     1441 ggtcgttctg ccgcgtgaga atcaggaagt tggtggagag tccggcgtcg tatcggtcgc
     1501 gctcggcggc cagctgcacc tccgcggcct cgcgtccgga gcgcgcggcc tcgatgcgct
     1561 gccccgcggt ttccacggcc gccgtcgcgt ccaggacctc ggcgcggatc tgcttcttga
     1621 tccgcccggc gtcggccgcg gcctgccgtt ccgcgtggcg cgcgatcgcc gcgtttccgc
     1681 gcgcctcgcg gttcaagatg ggaagctgga gctcgacccc gacgcgcgcg gcgtgatagc
     1741 cgcctcgctc cagcgagtca aaggagcgat cccagcggcc atccagctcg ggcgggagcg
     1801 tcccgcccga tccggcgagt ccggacaggc cgaagcggtc gtagcggagg acggcgtcca
     1861 gccgaggcca gatcccgtct tggtagtagg acgcctcggc acggcggcgc tcgatgaccg
     1921 actcggccac gtcgagctcg gggcgctgcg cgaaggcgtg atcgagtgcg cgcgccacgt
     1981 ccaccggcgc cggctcggga tcgtcggtct cgacgggtgc cagatcctcg gaccagagcg
     2041 cgtcgtccgc cccgtccagg atcaggagct tgagcgcgtt ctcggcgcgg gcgagcgctt
     2101 cgcgggcggc aaagaggtcg ccgcgccggc gctccagctc ggcccggggc tgcgccagct
     2161 ccgtcttggg cgcctctccg atgtccaccc gatgcccggt ctcgtccagc tgcgactgcg
     2221 cgaggtccac cgcctcctgc cgcaccccca cttccagccg cgcctccgtc agggtccagt
     2281 aggcacgctc cacggacgcc acggtctccg agacggagcg gcgcagcgaa gccgccgcac
     2341 ccgcccgccc cgcacgcgcc acccgcaccg agagccgcgc cgcgtcggtg ttccggtcgc
     2401 gaagcagcgg ctggcgcagc tgggcgccga cctgggtgcg gtacgcgggc gagaggaagg
     2461 cgagcccctc gcccaccgtc tcccgcgagc cctgggcgtg cagcgacaac gctccgcccg
     2521 tgggaagaag ctggcggagc gacacggccg cgtcgccggt ctcgacttcg ggcgcgatca
     2581 cgcccagcgg cgagccgtag agcacggtcg gctcggtgct ccgggaccat cctccctgcc
     2641 cttcgagcac cgggtcgtac gcgccgtgcg cgcgccgcac cgcggccttg gcggaggcga
     2701 gcgattcgcg atcgatcacg aggccttcgt tccgccgcag cgacatctcg atcgcttcgt
     2761 tcagcgagag cgagcggccg gcgacgggcg ccggcatcgc ggcgagcatg gctcccaggg
     2821 cgagggccgt gatggttccg agttcgaggg cgcggcgcgt catgacacgc cctccttcaa
     2881 gtggctcccg ccgtgaccga ggtcgcaccg ctggagccgg tcgtcgatgt aacgggagaa
     2941 gcgcgcgagc accttgggat ccagccccgc gagggagatc tgctcgccgg cggtgagctc
     3001 catgcctcgc tggatcagct cggtcaggac ggcacgcgcg tcgtgagtga agcggcggcg
     3061 cagggcctcg tccgtcgcca ggattccgat gacacgctcg acattcactt ggctcatggg
     3121 ggtctccttt cacgagcctt ctatgtcaat cggcgtgcca cgtcgctgtt cttgccatat
     3181 gtcggtcgat cccgatgcgg cgcatggcat ttcggtctga tccgcgaacg cgaggccgag
     3241 acgcgatgtg tcgggggttg tcgataaatc gacagtccgt cgatttccac gcgcacgggt
     3301 tgcaggagta gcggcgattc ctacgggcgg tgaaacgcaa aacgccgggc tccccgaagg
     3361 gaacccggcg cggggtgagg atggggcggg actgggaagt taggaacggt cgctttccgc
     3421 gcgggcagtt cgccgcgcgg gcactctatt gcacgccgta cttctcgagc cgtgagtaga
     3481 gctgggcgcg cgagagaccc aggagacgcg cggccttcgt cttgttcccc ttggcctgac
     3541 cgagcgcgcg ttccaggaac ccgcgctcga ccgcggcgag gtcgatgccg tcgaccggaa
     3601 gcgccagcgt atcgacgcca ttcccgttcg gcgccggcga acgccgcgga ggcgtcggga
     3661 gatgctcgcg cgtgatcagg ccgccgtcgc agagaaggat cgcgcgctcg atcgcgt
//
LOCUS       DASSLZ010000098         7972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1429153, whole genome shotgun sequence.
ACCESSION   DASSLZ010000098 DASSLZ010000000
VERSION     DASSLZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429153"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VFM00_03620"
     CDS             complement(<1..1296)
                     /locus_tag="VFM00_03620"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HET7497364.1"
                     /translation="MFSRHRQLFASAVFALDALLIFGSWMAAYWARFHLLPLAAPLGI
                     PPMRTYLWMGAVLTPVSLLVLRSFRLYRSARTARLSQELLAVLQAIAIVAALAALGSY
                     FLRGEVARSVLLLFAVLAAFALCVARVAVRSALRRLRRQGLNLRHVLIVGTGPMAAHV
                     ARKMTLHADYGFQVHGFVSAAPRAEPGAEVAGLPVLGDVEHLPELVEMTGAQLVYLAL
                     AREEYEAELLALERLSDSTAAVRLVPDLARAFTLNASVEDFDGMPVVLVTESPDQGWN
                     GVLKRAFDVVFAGAGLLVLWPVLLAIAAWIKLDSRGPVFFVQDRVGLNGRRFRMLKFR
                     TMVPEAESDGARWTKKDDPRRTRAGAVLRKLSLDELPQLWNVFVGDMSLVGPRPEQPL
                     FVHQFRASVPRYMLRHHVKAGITGWAQVNGLRGDTPLERR"
     gene            complement(1320..2438)
                     /locus_tag="VFM00_03625"
     CDS             complement(1320..2438)
                     /locus_tag="VFM00_03625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7497365.1"
                     /translation="MSEYGLLLISALVSAVVNLIGLLRRGPVRRILVVKLDHLGDVIT
                     ATPALQALRRAHPEAEIDLLIAPSVAPLFEGLPFLTRVRTYDSPRYRRAGGDARPSPP
                     VPEGARYDVVVELRGDARTLFLPFQVGATRRLDRGTARLRDWIRRRLHPERPPLHEVE
                     ANLAIVQPLLGVRASAAGARVQVNVPASARESLARRLAEAGVPADRPLVALHPGASWA
                     PRAWLPERFAAIADWIEANYHAQVVVVGSAGERDVEAAVRSHARGSALSWFGTLSIVE
                     AAALLQRSRLLIGNDGGIAHLAAACGTPVAALFGPQDPRRFRPWAERVVALHHRVPCF
                     PCTQVRCVRPELPCVNLIEIAEVEAAARALLGPPPLRS"
     gene            complement(2435..3688)
                     /locus_tag="VFM00_03630"
     CDS             complement(2435..3688)
                     /locus_tag="VFM00_03630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7497366.1"
                     /translation="MPRPAALVLTPRFPWPLDDGGRIVLWQSVWSAARTHDVTLISFV
                     PPAEVSAPVAEPVAAACARIVRVAHRPPPLPVAAWRGALGRWPFTLARYQDPAFARAV
                     REAVARDRPEFALANHLHLAPYVEDLSGVPMVLREHNAEHRWMERQAKDRGPTAAGLY
                     ARLQAGRLRRAERELCERAALVLAIQEGEAASLRGLAPEARVRTLPVGIDLGRFLEPS
                     PVRPPVVLLPGSFAWAPNVEGALRFLEEGWPRIRAEAPEARLRLAGKAPPAPLRAAAS
                     RAGDRVQVAADVPSMEEEYSRATVVLVPLWVGAGARVKIVEAMAARAPVAATSLAAEG
                     LDLEPELHYACADAPRELADAVVRLLRNPAERLRLGAQGRALAEARWSLESTARLQAA
                     LIGEVVGRVGSSRPERPAGEAALRT"
     gene            complement(3633..4892)
                     /locus_tag="VFM00_03635"
     CDS             complement(3633..4892)
                     /locus_tag="VFM00_03635"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET7497367.1"
                     /translation="MSRSLLDQAAAFSFLLLAAALPWSIAPISIAVVLCGVLTLAAWW
                     SPGGQRWQRTPVDLPALGWIAALAIASVFALEPARAFPRVTKGLLLAIVPVAAYHARD
                     PKILKRAIVALLISAVPATVYALVRFAHDGGVFPARVKGLVGHALTYGGQATLLATVA
                     MALVARGPRRWKIGAAAFLLLLAPALIGSYTRSAWIATLVAGAVILGRLRARFLPILA
                     GAAVLALFLLPQHYRERALSAFDTHSVWNVERLYMWDAGARMFRDHPLTGVGIQDLSG
                     LYRRYRVPQATEDHGHLHNVVVQVGATMGVVGLAALTWLLIGLFRTAGHRWGAPLPRG
                     DLASAVRLAAVAGLVAFLVAGMFEWNLGDEELLDFLCVLVGLGFAASSSAAGLEAGSA
                     RSRAGVPSVDAPPRGARSDPSISVAPG"
     gene            4979..5980
                     /locus_tag="VFM00_03640"
     CDS             4979..5980
                     /locus_tag="VFM00_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HET7497368.1"
                     /translation="MEPISQDRMEPLIDPSSRIVVTGGTGFLGTHLLNALREAGYANT
                     MGFGSAAFDLTREGDVQRLLEETRPDAIVHLAAAVGGIGANRRHPGSFFYRNLMMGAL
                     LMEHARRAGVRRFLSVGTICSYPKFTPVPFREEDLWNGYPEETNAPYGLAKKMLLVQS
                     QAYRDEYGFDASNVLVVNLYGPGDNFDPESSHVIPALIRKCVLAADAGLPEIEIWGTG
                     NATREFLYVEDAARGIVAALERLEGSEPVNIGAGFEISIRDLAARIAEATGYRGRFRW
                     DPTKPDGQPRRSLDTSRAERLLGWTATTSFEEGLARTVAWFKARTTAEAPRESPLPR"
     gene            5923..6969
                     /locus_tag="VFM00_03645"
     CDS             5923..6969
                     /locus_tag="VFM00_03645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7497369.1"
                     /translation="MVQGADDGRSSARVAAPTLSVPRFSVVVPTRGDGEKLQKLFLAL
                     ERQTLAKDQFEIVLAFDGAPVPPAIAAGGLNAQSAARAVALPERRGPGAARNAGARAS
                     RGEWIAFTEDDCEPAPDWLEAAAARIAREPALEALEGATLLPDGKPARRRLDDAPTWL
                     PTNLFVRRDLFERSGGYVEGFFDRRRGIYFREDSDFGFTLAALPARVGIEPAARVVHP
                     REHPTWIDPIRWARRYEMDPLLASRHPEAFREGIEVWRLGPIRIRRPFVRACGAYVLA
                     LVLAGAAALIGEGGVASWFLGLAALAFLVVWAKWRLDPRKAPAALVVPFVLLAALARG
                     KMRVYTGARPNSAA"
     gene            complement(6936..>7972)
                     /locus_tag="VFM00_03650"
     CDS             complement(6936..>7972)
                     /locus_tag="VFM00_03650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7497370.1"
                     /translation="GRRERAADGYRLRTGAVIWPGYENRGFYVSGLLGAMRRARPEIV
                     HLWEEPFSLMALQALAARRMAAPRAQALFFTSDNLSRGFRYPYRPSPLYAAIERWVHG
                     QCVAAGAVTEEVRDVLRSKGYRGPIDVMPHALDLADYPEPTPERRQAARARLGARGVV
                     IGYAGRLLAVKGLNLLLRAAALALRSGGDATVVIAGVGPERERLRALAAELTLGERAR
                     FVDPVPHEAMPDLLAAFDITAMPSITTPSATEQFGRVALEAMAAGSAVVVSSSGGLPS
                     VVGDAGIVVPEGDVAALAGALERLIASPAERASLATRARARVRERFTWDRIAEALAAR
                     YAALLGRAPV"
BASE COUNT         1217 a         2968 c         2852 g          935 t
ORIGIN      
        1 ccgccgctcg agcggcgtgt cgccgcgcag cccgttcacc tgtgcccagc ccgtgatccc
       61 cgccttcacg tgatggcgca gcatgtagcg cggcaccgaa gcgcggaact ggtgcacgaa
      121 gagcggctgc tcgggacggg gccctaccag gctcatgtcg ccgacgaaca cgttccagag
      181 ctgcggcagc tcgtccagcg agagcttccg gagcaccgcg ccggcgcgcg tgcggcgcgg
      241 atcgtccttc ttggtccacc gcgcgccgtc cgattcggcc tccggcacca tggtgcggaa
      301 cttgagcatc cggaagcgcc ggccgttcag ccccacgcga tcctggacga agaagacggg
      361 gcccctggaa tccagcttga tccacgccgc gatcgcgagc aggaccggcc acaggacgag
      421 cagcccggcg cccgcgaaga cgacgtcgaa ggcgcgcttc aggacgccgt tccagccctg
      481 gtcggggctc tccgtgacca ggaccaccgg catgccgtcg aagtcctcga cgctcgcgtt
      541 cagcgtgaac gcgcgggcca ggtcgggaac cagccgcacc gcggccgtgg aatcgctgag
      601 ccgctccagc gcgagaagct cggcttcata ttcctcgcgc gccagcgcga ggtacaccag
      661 ctgggcgccc gtcatctcca ccagctccgg caggtgttcc acgtccccga gcacgggcag
      721 cccggcgact tcggcgcccg gctcggcgcg cggcgccgcg ctcacgaaac cgtgcacctg
      781 gaagccgtag tcggcgtgca gcgtcatctt ccgcgccacg tgcgccgcca tcggccccgt
      841 tcccacgatc agcacgtggc gcaggttcag cccctgccgc cggagccggc gcaacgcgga
      901 gcgcacggcg acgcgcgcca cgcagagcgc gaacgccgcc aggaccgcga agaggaggag
      961 cacggagcgc gcgacctcgc cccggaggaa gtaggaaccg agcgccgcga gcgccgcgac
     1021 gatcgcgatc gcctggagca ccgcgagcag ctcctgcgag agccgcgccg tgcgcgccga
     1081 tcggtagagg cggaaactcc gcagcacgag gagggacacc ggcgtgagca cggcacccat
     1141 ccagagatag gtgcgcatgg gcggaattcc gagcggcgcc gccagcggaa gcaggtgaaa
     1201 gcgcgcccag taggccgcca tccaggagcc gaagatgagg agggcgtcca gggcgaacac
     1261 ggcgctggcg aagagctggc ggtgcctcga gaacatgcgg cctcagtggc tccgggtggt
     1321 cacgatcgaa gcggcggcgg tccgagaagg gcgcgcgcgg ccgcctccac ctcggcgatc
     1381 tcgatcaggt tcacgcaggg cagctccgga cggacgcatc gcacctgggt gcagggaaaa
     1441 caggggacgc gatggtgaag tgcgacgacc cgctcggccc acgggcggaa ccgccgcggg
     1501 tcctgcggcc cgaagagcgc cgccaccggc gtgccgcatg cggccgccag gtgcgcgatt
     1561 ccaccgtcgt ttccgatgag gagccgggat cgctggagca gcgcggccgc ctcgacgatg
     1621 gagagcgtcc cgaaccacga gagggcgctc ccgcgcgcat gcgaccgcac cgcggcctcg
     1681 acgtcccgct cccccgccga gcccacgacg acgacttgcg catgatagtt cgcctcgatc
     1741 caatcggcaa tcgcggcaaa ccgctccggg agccaggcgc gcggcgccca cgaggcgccg
     1801 ggatgaagcg ccaccagggg ccggtccgcc gggacgccgg cttcggcgag ccgtcgcgcc
     1861 aacgactcgc gcgcggaagc gggaacgtta acctggacgc gcgcgccggc tgcgctcgcg
     1921 cggacgccga gcagaggctg gacgatcgcg agattcgctt ccacctcgtg gagcggcggc
     1981 cgctcgggat ggagccgccg cctgatccag tcgcgaagcc gcgccgtgcc gcgatccagc
     2041 cggcgcgtcg cgcccacctg gaacggaagg aataacgtgc gcgcgtcgcc ccgcagctcg
     2101 accaccacgt cgtagcgcgc gccttccggc accgggggcg agggccgcgc gtcgccgcct
     2161 gcgcggcgat agcgcggcga atcgtacgtc cgaacccgcg tgaggaacgg cagcccctcg
     2221 aacaggggcg cgaccgaggg cgcgatcagg aggtcgatct ccgcctccgg atgagcgcgg
     2281 cgcagcgcct ggagcgccgg ggtggcggtg atcacgtcgc ccagatggtc cagcttcacg
     2341 accaggatcc ggcgaaccgg tccgcggcgc agaagcccga tgagattcac gacggcggag
     2401 acgagcgcgg agatgaggag gagcccgtac tcgctcacgt gcggagcgcc gcctcccccg
     2461 ccgggcgctc gggtcgcgac gatccgaccc gcccgacgac ctccccgatc agggccgcct
     2521 ggaggcgcgc ggtcgattcc aaggaccacc gcgcctcggc cagcgcgcgc ccctgcgcgc
     2581 ccaggcggag ccgctcggcg ggattgcgca ggagccggac gaccgcgtcg gccagttcgc
     2641 gcggcgcgtc ggcgcacgcg tagtgaagct ccggctccag gtccagcccc tcggcggcga
     2701 gcgaggtggc ggcgaccgga gcgcgcgcgg ccatcgcctc gacgatcttc acgcgcgcgc
     2761 ccgcgccgac ccagagcggc accagcacga ccgtcgcgcg cgaatactcc tcctccatcg
     2821 acggcacgtc ggccgcgact tggacgcggt cgccggcgcg cgacgccgcc gcgcgcagcg
     2881 gggccggagg ggccttcccg gccagacgca acctggcctc gggcgcctcc gcgcggatcc
     2941 ggggccagcc ttcttcgaga aagcggagcg ccccctcgac gttgggcgcc caggcgaacg
     3001 atccgggaag gagcaccacc ggcgggcgga cggggctggg ctccaggaag cgtccgagat
     3061 cgattccgac cggaagcgtt cgcacgcgcg cttccggagc gagaccgcgg agcgacgccg
     3121 cttccccctc ctggatcgcg agcacgagcg ccgcgcgttc acacagctcc cgctccgcgc
     3181 gccgcagccg tccggcctgg agccgcgcgt agagtcccgc cgcggttggg ccgcggtctt
     3241 tggcctggcg ctccatccac cggtgttcgg cgttgtgctc gcgcagcacc atcggcaccc
     3301 cgctcaggtc ctcgacgtag ggggccagat ggagatggtt cgccagcgcg aactccggcc
     3361 ggtcgcgcgc caccgcctcg cggacggccc gcgcgaacgc cgggtcctgg tagcgcgcga
     3421 gcgtgaaggg ccagcgtccc agggcgccgc gccaggccgc gaccggaagc ggcggcggcc
     3481 ggtgcgccac gcgcacgatc cgcgcgcacg cggcggcgac gggctcggcg accggggcgg
     3541 agacttccgc cggcggcacg aacgagatca gcgtgacgtc gtgggtgcgg gccgcggacc
     3601 agacgctctg ccagaggacg atccgtcctc cgtcatccag gggccacgga aatcgagggg
     3661 tcagaacgag cgccgcgggg cggggcatcc accgatggta ccccagcgcg ggaccgggcg
     3721 cttcccgcct cgagaccggc ggcggaggag gatgcggcga accccaggcc caccaggacg
     3781 cagaggaagt cgagcagctc ctcgtcgccc aggttccact cgaacatccc ggccacgagg
     3841 aacgcgacga gcccggcgac cgccgcgagg cgcaccgccg aagccaggtc gccgcgcggg
     3901 agcggcgcgc cccagcgatg tcccgccgtg cggaagagcc cgatcaggag ccacgtgagc
     3961 gcggccagcc ccacgacccc catcgtcgcc cccacctgca ccaccacgtt gtggaggtgg
     4021 ccgtggtcct cggtcgcctg cggcacgcgg tacctccggt agagcccgct cagatcctgg
     4081 atccccacgc ccgtgagggg atggtcgcgg aacatgcgcg cgcccgcgtc ccacatgtac
     4141 agccgctcca cgttccagac gctgtgggtg tcgaaagcgc tgagcgcgcg ctcccggtaa
     4201 tgctgcggca agaggaagag tgccaggacc gccgctcccg cgaggatcgg aaggaagcgc
     4261 gcgcgcagac gaccgaggat gaccgcgccc gcgaccagcg tcgcgatcca ggcgctccgg
     4321 gtgtaggagc cgatcagcgc gggagcgagg agcaggagga acgccgcggc gccgatcttc
     4381 cagcggcgcg gcccccgcgc cacgagcgcc atcgcgaccg tggcgaggag cgtggcctgg
     4441 ccgccgtagg tgagcgcgtg gccgaccagg cccttcaccc gcgccgggaa gacgccgccg
     4501 tcgtgcgcga accggacgag cgcgtagacc gtcgcgggca ccgcggagat cagaagcgcc
     4561 acgatcgcgc gcttcaaaat cttcggatcg cgcgcgtgat acgccgcgac cggcacgatc
     4621 gcgaggagga gccccttcgt cacgcgcggg aaggctcgcg ccggttcgag cgcgaacacg
     4681 gacgcgatcg caagagccgc gatccagccg agcgcgggaa ggtccacggg ggtccgttgc
     4741 cagcgctgac cgccgggcga ccaccaggca gcaagcgtca gcacgccgca gagcaccacc
     4801 gcgatcgaga tcggcgcgat cgaccacggg agcgccgcgg cgagcaggag gaaggagaag
     4861 gcggcagcct ggtccaggag ggagcgggac atgagcccgc atcgtcgcgc atgccccagc
     4921 gagggtcaag cagggcgctt gacgcctccc gggcgctgcg gcacgatcag cgcctcccat
     4981 ggaaccgatc tcccaggacc gcatggagcc gctcatcgat ccctcgagcc gcatcgtcgt
     5041 gaccggcggc accggattcc tcggcacgca tctcctgaac gcgctgcgcg aggcgggcta
     5101 cgcgaacacc atggggttcg gctccgccgc cttcgacctc acgcgcgagg gcgacgtgca
     5161 gcgcctgctg gaggagaccc ggcccgacgc gatcgtgcac ctcgccgccg ccgtcggcgg
     5221 aatcggcgcc aaccgccggc accccggctc gttcttctac cgcaatctca tgatgggcgc
     5281 cctcctcatg gagcacgcgc gccgcgccgg cgtgcgccgc ttcctctccg tgggcaccat
     5341 ctgcagctac cccaagttca cgccggtccc cttccgcgaa gaggacctct ggaacggcta
     5401 tcccgaggag accaacgcgc cctacgggct cgcgaagaag atgctcctcg tgcagtccca
     5461 ggcctaccgc gacgagtacg gcttcgacgc ctcgaacgtg ctcgtggtga atctctacgg
     5521 cccgggcgac aacttcgacc ccgagtcctc gcacgtgatc ccggcgctga tccggaagtg
     5581 cgtgctggcg gccgatgccg gcctgcccga gatcgagatc tggggcacgg ggaacgccac
     5641 gcgcgagttc ctgtacgtcg aggacgcggc gcgcgggatc gtcgccgcgc tcgaacgtct
     5701 cgaggggagc gagccggtca atatcggggc cggcttcgag atctcgatcc gcgatctcgc
     5761 ggcgcgcatc gccgaggcga ccggctatcg cggccggttc cggtgggatc ccaccaagcc
     5821 ggacggccag ccgcggcgct cgctggacac ctcgcgcgcc gagcggctcc tgggctggac
     5881 cgcgaccacc tcgttcgaag aggggctggc gaggacggtg gcgtggttca aggcgcggac
     5941 gacggccgaa gctccgcgcg agtcgccgct cccacgctga gcgttccccg cttcagcgtc
     6001 gtcgtcccca cgcgcggcga cggcgaaaag ctccaaaagc tcttcctcgc actcgaacgc
     6061 cagaccctcg ccaaggacca gttcgagatc gtgctcgcgt tcgacggcgc gccggtcccg
     6121 ccggcgattg ccgcgggcgg attgaatgcg cagagcgcgg cgcgcgcggt ggcgctgccc
     6181 gagcggcgcg gccccggcgc cgcgcggaac gccggcgctc gcgcctcgcg cggcgaatgg
     6241 atcgcgttca ccgaagacga ttgcgagccc gcgcccgact ggctcgaggc cgcggcggcg
     6301 cggatcgcgc gcgagcctgc gctggaagcg ctggagggcg ccacgctcct ccccgacggg
     6361 aagcccgccc gccggcggct cgacgacgcg cccacgtggc ttccgacgaa cctcttcgtg
     6421 cgccgcgacc tcttcgagcg ctcgggcgga tacgtcgagg ggttcttcga tcggaggcgc
     6481 gggatctact tccgggagga ctccgatttc ggcttcacgc tcgccgcgct cccggcgcgg
     6541 gtcgggatcg agcccgcggc gcgcgtcgtc catccccggg agcatcccac ctggatcgac
     6601 ccgatccggt gggcgcgccg ctatgagatg gacccgctcc tcgcctcgcg gcatcccgaa
     6661 gcgtttcgcg aagggatcga ggtgtggcgc ctcggtccga tacgcatccg gcgccccttc
     6721 gtgcgcgcct gtggcgccta cgtgctggca ctcgtcctgg ccggcgccgc ggccctgatc
     6781 ggagagggcg gcgtcgcgtc gtggttcctc ggcctcgcgg ccctggcgtt cctggtcgtc
     6841 tgggccaaat ggcgcctcga tccgcggaaa gcccccgcgg cgctcgtcgt gcccttcgtc
     6901 cttctcgccg cgctcgcgcg gggaaagatg cgggtctaca cgggcgcgcg acccaatagc
     6961 gccgcgtagc gggcggccag cgcctccgcg atccggtccc aggtgaagcg ctcccgaacg
     7021 cgggctcgag cgcgcgtggc gagcgacgcg cgctcggcgg gcgaggcgat cagccgctcc
     7081 agcgccccgg cgagcgcggc gacgtctccc tcgggcacca cgattcccgc gtcgcccacg
     7141 accgagggca gccccccgct gctcgagacc actaccgccg atcccgcggc catcgcctcc
     7201 agagcgacgc ggccgaactg ctcggtcgcg ctcggcgtcg tgatcgaggg catggcggtg
     7261 atgtcgaagg cggcgaggag atcgggcatc gcctcgtgcg gcacggggtc gacgaaccgc
     7321 gcccgttcgc ccagcgtaag ctcggccgcg agcgcccgaa gccgctcgcg ctcaggcccc
     7381 accccggcga tcaccacggt ggcgtcgcct cccgatcgga gcgccagagc ggcggcacgg
     7441 aggagcaggt tcagcccctt caccgcgagc agccgtcccg cgtagccgat caccacgccg
     7501 cgcgcgccca gccgtgcccg cgcggcctgc ctcctctccg gcgtcggctc gggatagtcg
     7561 gcgagatcca gcgcgtgggg catcacgtcg atcggcccgc ggtatccctt ggaacgcagg
     7621 acatcgcgca cctcttccgt gacggcgccc gccgcgacgc actggccgtg cacccaccgc
     7681 tcgatcgccg cgtagagggg cgaagggcga tacggatagc ggaacccgcg cgagaggttg
     7741 tcggacgtga agaagagcgc ctgggcgcgc ggcgcggcca tgcggcgcgc ggccagcgcc
     7801 tgcagcgcca tcaggctgaa cggctcctcc cagagatgca cgatctcggg gcgcgcccgg
     7861 cgcatcgcgc ccaaaagccc gctcacgtag aacccgcggt tttcgtaccc gggccagatc
     7921 acggctccgg tccgcaaccg gtagccgtcg gccgctcgct cgcgccgccc cg
//
LOCUS       DASSLZ010000099        11149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1049767, whole genome shotgun sequence.
ACCESSION   DASSLZ010000099 DASSLZ010000000
VERSION     DASSLZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049767"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFM00_03655"
     CDS             <1..606
                     /locus_tag="VFM00_03655"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7497371.1"
                     /translation="NLLLPANSAVVTTTPLTLRWRSTTDPQLEALTYTVTLRSVATNQ
                     VVFTKTTTDSSAVVTGYIGPNTTYEWSVVATDALSHGRSARERFRFTTGATTDVVEIP
                     PSASAVVLGQNRPNPFLRTTDIPFTLAGSGDPVAAELRVFDARGRLVKTLVAGDRPTA
                     RQYLIRWDGRDDTGHPAAAGIYFYRLRAAGREMVRRLVLVR"
     gene            673..1425
                     /locus_tag="VFM00_03660"
     CDS             673..1425
                     /locus_tag="VFM00_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7497372.1"
                     /translation="MSGHSKWATIKRKKAATDAKRGKIFTKYIKEIIVAARAGGGDQN
                     ANPRLRTAVLGAKSVNMPADNIERAIKKGTGELEGVNYEEVTYEGYGPGGIAILMDSL
                     TDNRNRTTGELRHILTKAGGRMAEAGAVQWMFHAKGQIVIPRSAIDEESLIELVLDAG
                     AEDVATDDPETYEIMTPLAQFEAVKAALAKKGITPTSAEIAKVPQNTIALSEKDAEQA
                     LKLMEALEDHDDVQRISSNLDIADEVLSKFQQ"
     gene            1469..1972
                     /gene="ruvC"
                     /locus_tag="VFM00_03665"
     CDS             1469..1972
                     /gene="ruvC"
                     /locus_tag="VFM00_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET7497373.1"
                     /translation="MDPGSRATGYGLVRLEGSRVHYERSGAIAPRASLSFADRLVAVH
                     DALAHLLGELVPDEVAVESAFVRKSAKAALQIGHVRGVVLLAARKSGAVIFEYTAPEV
                     KMAVVRQGAAAKEQVAFMVRRLLPGVGAVTEDEADALAVALCHAHRSPLVTSARVAIP
                     LRRFSRS"
     gene            1969..2580
                     /gene="ruvA"
                     /locus_tag="VFM00_03670"
     CDS             1969..2580
                     /gene="ruvA"
                     /locus_tag="VFM00_03670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET7497374.1"
                     /translation="MIDALSGTLVSKMPTSAVVRMGGISIRVHISVSCFETLPAVGQT
                     LSLLTYLHVREDALQLYGFADHVERELFERMIAVSGIGPRLAVGILSGATATRVREAL
                     QAGDAEFLQTLPGVGKKLAQRLVVELSEKAPAMGGAVPAAAGTPGALGAHTTVVADAA
                     AALTALGFSRSGAMGAAQDAVQELGAAATVESVVRRALQQKVR"
     gene            2647..3765
                     /gene="ruvB"
                     /locus_tag="VFM00_03675"
     CDS             2647..3765
                     /gene="ruvB"
                     /locus_tag="VFM00_03675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET7497375.1"
                     /translation="MPRPASPEPKKRRVAAGAPVPVPAPDRERVTDPELLGNDRELEG
                     SLRPRQLSDFTGQDGLREQLRIATEAARARGEALDHILFHGPPGLGKTTLASILAAEM
                     DVPFVQTSGPVLERAADLAGILTNLDERGILFIDEIHRMNPVVEEYLYPAIEDFCLDI
                     MIDRGPSARSVRIDLKRFTLVGATTRTGLLSAPLRGRFGLSARLDYYSREELTRIIRR
                     SASILGVSIDAGGAREIASRARGTPRVGNRLLRRVRDYAETRADGTITAAVAQAALTL
                     LEVDARGLDDMDRRMLEAIVVKYGGGPVGLSTIAVVVGEEPDTLEDVYEPYLIQEGFL
                     KRTPRGREATDLAFAHLGRSRTALPAPDSEGQQRLEGF"
     gene            3927..4820
                     /locus_tag="VFM00_03680"
     CDS             3927..4820
                     /locus_tag="VFM00_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7497376.1"
                     /translation="MSTTAAVGRIPDIVQRHERELLAEWIQLQIASMGFQRDRGREDE
                     LTDQSRRFLRHFTGSLQAGGASADIRTPAWAETREFLGDLSRSRATQGYSPTETAGFI
                     FSLKQPLFARMRQELRDSDQLAEQLWTATMILDQLGLYTTEVYLKGRDEVIRRQETEM
                     TELSTPVVELWEGVLALPVIGTLDSSRAQTVMENLLQKIVETGSSYAIIDITGVPTVD
                     TLVAQHLLKTIAAARLMGAECIISGIRPQIAQTIVHLGVELQGVATKSTLADAFAIAL
                     ARRGLTVASLNGAGPPPRPAR"
     gene            4831..5241
                     /locus_tag="VFM00_03685"
     CDS             4831..5241
                     /locus_tag="VFM00_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7497377.1"
                     /translation="MERIPILKMGDCLLVTIQVDMHDRLAMTLQDDLTSRIAETRARG
                     VVIDISSLEVVDSFIGRILANIAAMARVLDAETVVVGMRPAVAITLVELGLSLPGVRT
                     ALNVERGMDLIRRDDAAGPEAAGSAESSSGRSQG"
     gene            5222..5626
                     /locus_tag="VFM00_03690"
     CDS             5222..5626
                     /locus_tag="VFM00_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HET7497378.1"
                     /translation="MAVLRDEVREIRSSQDVVGVRQMVREWAVYAGFSLVDQTKIVTA
                     ASELARNTLTHGGGGSMRVEALNDGNRRGVRVVFEDQGPGIPNVEQALRDGFTTGGGL
                     GLGLGGAKRLCHDFEILSEPGRGTRVAITRWR"
     gene            5623..6621
                     /locus_tag="VFM00_03695"
     CDS             5623..6621
                     /locus_tag="VFM00_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7497379.1"
                     /translation="MTESRAIPVREISQTGQVRRAATVLAERMGFDSEETGRVGLVAT
                     EAATNLVKHAQGGEIVLSSVKDAETRAVDIVALDRGPGIRDLDRAMRDGFSTAGGMGG
                     GLGAIRRNSSHFDLYSAESGTILFARVGVGPGAPVGGGMDVGAVQVPCPGEQVSGDAW
                     AVAHHSDRTLVLVADGLGHGADANAAAELSMRLFLQHANGSPASILERIHEGLRPTRG
                     AAAAVLELDKVERVARFAGIGNIAASVHAAGESRSMVSHHGVLGHSLRKIHEFLYPWP
                     PRSLVVLHSDGIGTHWDLGRYPGLMQRRPAVIAAALYRDFCRGRDDATAVALREAA"
     gene            6618..8744
                     /locus_tag="VFM00_03700"
     CDS             6618..8744
                     /locus_tag="VFM00_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7497380.1"
                     /translation="MSTRILSLALRQEVDVVLARQRARRIASLLGFEGQDQVRIATAV
                     SEIARNAVAYAEQGMAEFAIEGTTPPQVFTIRITDKGPGIRRLPDILEGRYRSTTGMG
                     IGISGARRLMDQFSIASEPGHGTTVTLKKLVPPGMPLITPAGIAVIADSLATEKPQGA
                     FEEIQRQNQELLATMSELEQRQEELLVLNRELEDTNRGVVALYAELDEKADHLRRADE
                     LKSRFLSNMTHEFRTPVNSIQALAWMLLERSDGPLTSEQERQVQFIRRASDELSELVN
                     DLLDLAKVEAGKSEVRPTLFEVSNLFGALRGMLRPLLVTTSVALVFEEPEGIPPLHTD
                     EGKVSQILRNFISNALKFTEQGEVRVRARLVNGGGKIAFEVSDTGIGIAPEDQERVFQ
                     EFTQIDSAIQRRVRGTGLGLPLCRKLTELLGGSVSVRSEVGSGSTFIAEIPVSYAERD
                     SPAFESWHAIPGRLPVLVVEDAPEEALLYEKYLSAAGYQVLHARTLREAREALTVVNP
                     ALILLDVLLRGEDTWALLTELRRRPDTGETPILIVSTVEDQAKGASLGANAYLLKPID
                     RQRLIQQVSSMTRSVTMSRVLIVDDEEISRYLIRQSLAAPHVEVLEAATGAEALHVAG
                     SGRIGAICLDLRMPDQDGEEVLRRLKSDPTTCAIPVFVVTSKALGEGERDRLLEMAAG
                     VISKETLSRERILPRVEEAMRRTAAA"
     gene            8754..10373
                     /locus_tag="VFM00_03705"
     CDS             8754..10373
                     /locus_tag="VFM00_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7497381.1"
                     /translation="MAPKAELILVVDDNDTARYHKERILVRAGYEVIPAKDGLEALRL
                     VAEREPRVVVLDVKLPGLDGWEVCRRIKADPGTASTLVLQVSATYVSDADTVRALEGG
                     ADAALSEPIEPTVLVATVRSLLRVRRAEDSLRAALAGEQTARSAAEAARAAVEAANQS
                     KDEFLATLSHELRSPLGTVLSWVSLLRSANGDQAQMARGLEVIERNVRLQMKLIDDLL
                     DVSRIVSGKLRLDVALVGLEPIVAAAIVNIRGAAEAKHVRVESMVEDTAGPVWGDATR
                     LQQVVWNLLSNAIKFTPRDGTVRVSVRGADTGVRIEVADTGKGIAADVLPKIFERFHQ
                     ADPSTTRSEGGLGLGLAIVRQIVELHGGTVEARSPGLGGGTTMIVNLPPAEARGLAAV
                     RTTGGSTAKPVVSETPSLSRLRLLVVEDDPDALEAISAVLQRSGASVTSVRSVPEALI
                     ALGREKLDVLISDIAMSGMDGITLIRAVRERSRERGGAIPALALTAYTGGDTQRRALE
                     AGYQAYLTKPIDASDLVSAIASLAGVERPAG"
     gene            10370..>11149
                     /locus_tag="VFM00_03710"
     CDS             10370..>11149
                     /locus_tag="VFM00_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET7497382.1"
                     /translation="MTAGPLRTSDFDYHLPPDRIAQHPSAERDESRLLILPRRAGPNA
                     PGSSPAPGSSPAPESNLAPAIEHRRFRDLPSYLRPGDLLVVNDTRVIPARLFARLPRE
                     AGCASPREAELLLVREEPPFPGAPSERLWLALARPARRLRPGLPLAFADPSWSAVVSG
                     VEERGARRVAFRPQGTLSFESWLARVGHVPIPPYIERADEPEDRERYQTVYARHPGSV
                     AAPTAGLHFTEATLQACRDRGVTIASVTLHVGPGTFRPVATE"
BASE COUNT         1668 a         3936 c         3920 g         1625 t
ORIGIN      
        1 aatctcctgc tgccggcgaa ttccgcggtg gtgaccacca cgcccttgac gctccgatgg
       61 aggagcacga ccgacccaca gctcgaggcg ctcacctaca ccgtgacgct gcgatcggtg
      121 gccacgaacc aggttgtctt cacgaaaacc acgacggaca gctccgccgt ggtgaccggc
      181 tacataggcc cgaacaccac ctacgaatgg agcgtcgtcg ccaccgacgc gctgagccat
      241 ggccggagcg cccgcgagcg gtttcgattc acgaccggcg cgacgaccga cgtcgtggag
      301 attccgccct cggcttccgc cgtcgtcctg gggcagaatc gccccaatcc gttcctccgc
      361 acgaccgaca tcccgttcac cctcgcgggg agcggcgatc cggtggcggc cgagctgcgt
      421 gtgttcgacg cgcgcgggcg gctggtgaag acgctggtcg cgggcgaccg gccgaccgcg
      481 cggcagtact tgatccgctg ggacgggcgg gacgacacgg gtcacccggc ggcggccggg
      541 atctacttct accggcttcg cgcggccgga cgcgagatgg tccggcgact cgtgctggtg
      601 cgctgatcgc ttcacgccgc cggccgggcc cggccgcggc gagagtcgtc gtcgtcaagg
      661 ggggaacgca gcatgtcggg tcattcgaaa tgggcgacca tcaagcggaa gaaagccgcg
      721 acggacgcca agcgagggaa gatcttcacg aagtacatca aggagatcat cgtcgccgcg
      781 cgcgccggcg gcggggatca gaacgccaat ccgcgcctgc gcaccgccgt cctcggcgcc
      841 aagtcggtca acatgcccgc cgacaacatc gagcgcgcca tcaagaaggg gaccggcgag
      901 ctcgagggag tgaactacga agaagtgacg tacgagggct acggccccgg cggcatcgcg
      961 atcctcatgg attccctcac cgataaccgc aaccgcacga caggcgagct ccggcacatc
     1021 ctgaccaagg ctggcggacg gatggccgaa gccggcgccg tgcagtggat gttccacgcc
     1081 aaggggcaga tcgtcatccc gcgatcggcg atcgacgagg agtccctcat cgagctcgtc
     1141 ctggacgcgg gggccgagga cgtggccacg gacgaccccg agacgtacga gatcatgacg
     1201 ccgctcgcgc agttcgaggc ggtgaaggcg gcgctcgcga agaaggggat caccccgacc
     1261 tccgccgaga tcgccaaggt gccgcagaac acgatcgcgc tgtccgagaa ggatgccgaa
     1321 caggccttga agctcatgga ggcgctcgag gaccatgacg acgtccagcg gatctcgtcc
     1381 aatctcgaca tcgcggacga agtcctctcc aagttccagc agtagcgcgc ccgcgccggc
     1441 ggccgcggcg ggccggcgca ttctcgggat cgaccccggc agccgcgcca ccggatacgg
     1501 gctggtgcgt ctcgagggga gccgcgtcca ctacgagcgc tccggcgcca tcgcgccgcg
     1561 ggcctcgctc tcctttgccg accgtctcgt cgccgtgcac gacgctctgg cgcatctcct
     1621 cggagagctc gttcccgacg aggtcgccgt cgaatcggcc ttcgtccgga agagcgccaa
     1681 ggcggcgctc cagatcggcc acgtccgggg cgtcgtcctc ctggccgcgc gcaagagcgg
     1741 ggccgtgatc ttcgagtaca ccgcgcccga ggtgaagatg gcggtggtgc ggcagggcgc
     1801 cgcggccaag gaacaggtcg cgttcatggt gcgccggctc ctacccggcg tcggcgccgt
     1861 caccgaggac gaagccgacg ctctggcggt cgcgctctgc cacgcacacc gctcgccgct
     1921 cgtcacgtcg gcccgcgtcg ccatccccct ccggaggttc tcccggtcat gatcgacgcc
     1981 ttgagcggaa cgctcgtctc caagatgccc acctcggccg tcgtgcggat gggggggatc
     2041 tcgatccggg tccacatctc ggtctcctgc ttcgagacgc tccccgcggt gggccagacg
     2101 ctgtccttgc tcacctatct ccacgtgcgc gaggacgcgc tccagctgta cggcttcgcc
     2161 gatcacgtcg agcgcgagct gttcgagcgg atgatcgcgg tgtcggggat cgggccgcgg
     2221 ctggcggtgg ggatcctgag cggcgccacc gccacgcgcg tgcgcgaggc gctccaggcg
     2281 ggggacgccg aattcctcca gacgctcccc ggcgtgggca agaagctggc gcagcggctg
     2341 gtcgtggagc tttcggagaa ggcgccggcg atgggcggcg ctgtgccggc ggccgcgggc
     2401 acgccgggcg cgctcggcgc gcacacgacc gtggtggccg acgccgcggc cgcgctgacc
     2461 gcgctcggct tctcccggag cggcgccatg ggagcggccc aggacgcggt gcaggagctg
     2521 ggcgccgccg ccaccgtgga gagcgtcgtg cgccgggcgt tgcagcagaa ggtccgctag
     2581 cctaggcttc acccgttccc ccgttcccga tgacgtagcc aggccgctgg tacaccggag
     2641 tctcaaatgc cccgacccgc atcgcccgag ccgaagaagc gccgcgtcgc cgcgggcgcg
     2701 cccgtgcccg ttccggcacc cgaccgggag cgcgtgaccg atcccgagct tctcgggaac
     2761 gatcgcgagc tggagggcag ccttcgcccc cggcagctct ccgacttcac ggggcaggac
     2821 ggccttcgcg agcagctccg catcgcgacc gaggcggcgc gcgcccgcgg cgaggcgctc
     2881 gaccacatcc tgttccacgg gccgcccggg ctggggaaga cgacccttgc ctccatcctc
     2941 gccgccgaga tggacgtgcc cttcgtccag acttcggggc cggtgctgga gcgcgcggcc
     3001 gacctcgccg gcatcctcac caatctggac gagcgcggga tcctcttcat cgacgagatc
     3061 caccggatga acccggtcgt ggaggagtat ctctaccccg ccatcgagga cttctgcctg
     3121 gacatcatga tcgaccgggg ccccagcgcc cgcagcgtcc ggatcgacct gaagcgcttc
     3181 accctggtgg gcgcgaccac gcggaccggc ctcttgagcg cgcccctccg gggccgcttc
     3241 ggcctctccg cccggctcga ctactactcg cgcgaggagc tgacccggat catccgccgg
     3301 tccgcctcga tcctgggggt ctcgatcgac gccggcggcg cccgggagat cgcctcgcgc
     3361 gcccgcggca ccccccgggt gggaaaccgc cttctccggc gggtgcgcga ctacgccgag
     3421 acccgcgccg acgggacgat cacggccgca gtggcgcagg ctgccctcac cctgctcgaa
     3481 gtggatgccc gcggactgga cgacatggac cgccggatgc tggaggccat cgtcgtgaaa
     3541 tacgggggcg gcccggtcgg cctctccacg atcgccgtgg tcgtggggga ggagcccgat
     3601 accctcgagg acgtctacga gccctacctc atccaggagg ggtttctcaa gaggaccccc
     3661 cgcggccggg aggccaccga cctggcgttc gcccacctgg ggcggtcccg cacggccctg
     3721 ccagcccccg attccgaagg gcagcagcgg ctggaaggct tctagagcct ctttttccgg
     3781 gtaggactgc atttccgggt acgactaccc acaccgcgtg tggatggagc tttaccccct
     3841 tccaccactg gtcgtattct taccatccgg cttgaatgtc cgcctttcgc tgacaactgg
     3901 ttttctcgcc agggagatga cctcacatga gcaccacggc agccgtagga cgcattcccg
     3961 acatcgtcca gcgccacgag cgcgagctcc ttgcggagtg gatccagctc cagatcgcct
     4021 ccatgggctt ccagcgcgac cggggccgcg aagacgagct gacggatcaa tcgcgccggt
     4081 tcctgcgcca tttcacgggc agcctgcagg cgggcggcgc ctccgccgac atccgcaccc
     4141 cggcctgggc cgagacgcgg gagttcctgg gggacctctc gcgctcgcgc gcgacgcagg
     4201 gctactcccc gacggagacg gcggggttca tcttctcgct gaagcagccg ctcttcgcgc
     4261 gcatgcgcca ggagctccgg gattccgatc agctcgccga gcagctctgg accgcgacca
     4321 tgatcctgga ccagctgggg ctctatacga ccgaggtcta tctgaagggc cgggacgagg
     4381 tcatccgccg ccaggaaacg gagatgacgg agctctcgac gccggtggtc gagctgtggg
     4441 agggggtcct ggcgcttccg gtcatcggca ccctcgattc gagccgcgcc cagacggtga
     4501 tggagaacct gctccagaag atcgtggaga ccggctcttc ctacgccatc atcgacatca
     4561 ccggggtgcc gacggtggac accctggtgg cgcagcacct gctcaagacg atcgcggcgg
     4621 cacggctgat gggagccgaa tgcatcatca gcggcatccg tccccagatc gcgcagacga
     4681 tcgtgcacct gggcgtggag ctgcaggggg tggccaccaa gtccaccctc gccgacgcct
     4741 tcgcgatcgc gctggctcgc cgcggcctca ccgtcgcctc gttgaacggc gccgggccgc
     4801 ctccgcgtcc ggcgcgctga gggcggcccc atggaacgga tcccgatcct caagatgggc
     4861 gactgcctgc tcgtcacgat ccaggtcgac atgcacgacc ggctcgcgat gacgctgcag
     4921 gacgatctca cctcccgcat cgccgagacc cgcgcgcgcg gcgtcgtgat cgacatctcg
     4981 tccctcgaag tggtcgattc cttcatcggg cggatcctcg cgaacatcgc cgccatggcg
     5041 cgcgtgctgg acgccgagac cgtcgtggtc ggcatgaggc cggccgtggc gatcaccctg
     5101 gtcgagctgg ggctctcgct cccgggggtg cgcaccgcct tgaacgtgga gcgcggcatg
     5161 gatctgatcc gccgcgacga cgctgcaggc cccgaggcgg ctggatccgc ggagtcttcg
     5221 agtggccgtt ctcagggatg aggtccggga gatccgctcg tcgcaggacg tggtcggcgt
     5281 gcgccagatg gtccgcgaat gggccgtcta cgccggcttc agcctggtcg accagaccaa
     5341 gatcgtaacc gccgcgagcg agctggcgcg gaacacgctg acccacggag gcggcggttc
     5401 gatgcgggtg gaagcgctga acgacggaaa ccgtcgcggc gtccgcgtcg tgttcgagga
     5461 ccagggaccg gggattccca acgtggagca ggcgctccgc gacggcttca cgaccggcgg
     5521 ggggctggga ctggggttgg gcggcgccaa gcgcctctgc cacgatttcg agatcctctc
     5581 cgagccgggg cgcggcaccc gggtcgcgat cacgcgctgg cgatgaccga aagccgcgcg
     5641 attccggtgc gcgagatcag ccagaccggg caggtgcgcc gggcggcgac ggtgctcgcg
     5701 gaacggatgg ggtttgattc cgaggagacc ggccgcgtgg gcctggtggc gaccgaggcg
     5761 gccacgaacc tcgtgaagca cgcgcagggg ggagagatcg tgctctcgtc cgtgaaggat
     5821 gccgaaaccc gtgccgtgga catcgtcgcg ctggatcgcg gtcccgggat ccgcgatctg
     5881 gatcgcgcca tgcgggacgg cttctccacg gccggcggca tgggcggagg gctgggcgcc
     5941 atccgccgga actcctcgca tttcgacctc tacagcgcgg aatcgggcac gatcctcttc
     6001 gcgagagtgg gggtcggccc cggcgccccc gtgggcggcg ggatggacgt gggggcggtc
     6061 caggttccct gcccgggcga gcaggtctcg ggcgacgcgt gggccgtggc gcatcattcc
     6121 gaccgcacgc tcgtgctggt cgccgacggt ctgggccatg gcgccgacgc caacgcggcg
     6181 gccgagctgt cgatgcgcct cttcctgcag cacgcgaacg gctcccccgc ctcgatcctc
     6241 gagcgcatcc acgaggggct tcgccccacg cgcggcgccg ccgcggccgt gctcgagctg
     6301 gacaaggtgg agcgggtggc ccgattcgcc ggtatcggca acatcgccgc ttccgtccac
     6361 gcggcggggg aatcgcgatc gatggtcagc catcacggcg tgctggggca ctcgctccgg
     6421 aagatccacg agttcctcta tccctggccg ccgcgctccc tggtcgtgct ccactcggac
     6481 gggatcggaa cgcactggga cctcgggcgc taccccggtc tgatgcagcg ccgcccggcg
     6541 gtgatcgccg cggcgctcta tcgcgatttc tgccgtgggc gggacgatgc caccgccgtc
     6601 gcgcttcggg aggccgcgtg agcacgcgca tcctttcgct cgcgctccgt caggaggtgg
     6661 acgtggtgct cgcgcggcag cgcgcccggc ggatcgcttc gctcctcggc ttcgagggcc
     6721 aggaccaggt gcggatcgcc accgccgtct ccgagatcgc gcgcaacgcg gtcgcctacg
     6781 cggagcaggg gatggccgag ttcgcgatcg aggggacgac gccgccccag gtcttcacca
     6841 tccgcatcac cgacaaaggg ccgggcatcc gccggctgcc ggacatcctc gagggacgct
     6901 accgctccac gaccggcatg gggatcggca tctcgggagc gcgccggctg atggaccagt
     6961 tcagcatcgc ctccgagccg gggcacggca ccaccgtgac cctgaagaag ctcgtcccgc
     7021 ccggcatgcc gctcatcacc ccggcgggca tcgcggtcat cgccgattcg ctcgccacgg
     7081 aaaagccgca gggcgcgttc gaggagatcc agcggcagaa tcaggagctg ctcgccacca
     7141 tgtccgagct ggagcagcgc caggaggagc tcctggtcct gaaccgcgag ctggaggaca
     7201 ccaatcgcgg cgtcgtcgcg ctctacgccg agctggacga gaaggccgac cacctgcgcc
     7261 gcgccgacga gctcaagtcg cgcttcctct cgaacatgac gcacgagttc cgcacgccgg
     7321 tgaactcgat ccaggcgctc gcgtggatgc tcctcgagcg ctccgacggc ccgctcacct
     7381 cggagcagga gcgtcaggtc cagttcatcc gccgcgcttc cgacgagctg tcggagctgg
     7441 tgaacgacct gctggacctg gccaaggtgg aggccgggaa gtcggaggtg cgccccaccc
     7501 tcttcgaggt ctcgaacctc ttcggcgccc tgcgcggcat gctccgtccg ctcctcgtca
     7561 ccacctccgt ggccctggtc ttcgaggagc cggaggggat cccgccgctc cataccgacg
     7621 agggaaaggt ctcgcagatc ctgcgcaatt tcatctcgaa cgcgctcaag ttcaccgagc
     7681 agggcgaggt ccgcgtgcgc gcgcgcctgg tcaacggcgg agggaagatc gcgttcgagg
     7741 tgtcggacac cgggatcggg atcgcgccgg aagaccagga gcgggtcttc caggagttca
     7801 cccagatcga cagcgcgatc cagcggaggg tccgcggcac ggggctcggc cttccgcttt
     7861 gccgcaagct gacggagctc ttgggcggct cggtctccgt gcggagcgag gtgggctccg
     7921 gatccacgtt catcgcggag atcccggtct cctacgcgga gcgggacagt cccgcgttcg
     7981 aatcctggca cgcgataccc gggcgcctcc ccgtgctcgt ggtcgaagac gcgccggagg
     8041 aggcgctcct ctacgagaag tatctctcgg cggcaggcta ccaggtgctc cacgcgcgca
     8101 cgctgcgcga ggcgcgcgag gcgctgaccg tcgtgaaccc ggcgttgatc ctcctggacg
     8161 tcctcttgcg cggcgaggat acctgggcct tactgaccga gctccggcgc cgtccggata
     8221 cgggagagac gccgatcctg atcgtgagca ccgtcgagga ccaggccaag ggagcttccc
     8281 tgggagcgaa cgcctacttg ctcaaaccga tcgaccgcca gcgcctgatc cagcaggtct
     8341 cgtccatgac gcgtagcgtc acgatgagcc gcgtgctgat cgtggacgac gaggagatct
     8401 cgcgctatct gatccggcag agcctggcgg cgccgcacgt cgaggtcctg gaggccgcga
     8461 cgggggccga ggcgctccac gtcgccggct ccgggcggat cggcgcgatc tgcctcgatc
     8521 tccggatgcc ggaccaggac ggcgaggagg tgctgcgccg cctgaagagc gatcccacga
     8581 cctgcgcgat tcccgtgttc gtggtcacgt ccaaggcgct cggagaaggg gagcgcgacc
     8641 ggcttctcga aatggccgcg ggcgtgatct ccaaggaaac gctttcccgc gagcgcatcc
     8701 tgccgcgcgt cgaagaggcg atgcggcgga cggctgccgc gtaggagcga tcgatggccc
     8761 ccaaggcgga gctgatcctg gtcgtcgacg acaacgacac cgcgcgctac cacaaggagc
     8821 ggatcctggt gcgggcgggc tacgaagtga tcccggccaa ggacgggctc gaggccctgc
     8881 gcctcgtggc cgagcgggag ccccgcgtcg tcgtgctgga cgtgaagctg ccggggctgg
     8941 acggctggga ggtctgccgg cggatcaagg ccgaccccgg gaccgcctcc acgctggtct
     9001 tgcaggtatc ggccacctac gtgagcgacg ccgacaccgt acgggccctg gaagggggcg
     9061 ccgacgccgc gctctccgag ccgatcgagc ccacggtcct cgtggcgacc gtgcgctccc
     9121 tcctgcgggt gcgccgcgcc gaggactcgc tgcgcgccgc gctcgccgga gagcagacgg
     9181 cccggagcgc cgccgaagcg gcgcgcgcgg cggtcgaggc ggcgaaccag agcaaggacg
     9241 agttcctggc caccctctcc catgagctgc ggtcgcccct cggcacggtg ctgtcctggg
     9301 tctccctgct ccggtccgcg aacggggacc aggcgcagat ggcgcggggg ctggaggtga
     9361 tcgagcggaa cgtccggctg cagatgaagc tgatcgacga cctgctcgac gtctcccgca
     9421 tcgtctcggg aaagctgcgc ctggacgtgg cgctcgtggg gttggagccg atcgtggccg
     9481 cggcgatcgt gaacatccgg ggcgccgccg aggccaagca cgtgcgcgtg gaatcgatgg
     9541 tggaggacac cgccggcccc gtctggggag atgccacgcg gctgcagcag gtggtctgga
     9601 acctgctctc gaacgcgatc aagttcacgc ctcgggacgg caccgtgcgc gtgtcggtgc
     9661 gcggggccga cacgggcgtc cggatcgagg tggccgatac cgggaagggg atcgctgccg
     9721 acgtcctccc gaagatcttc gagcgcttcc accaggcgga tccctccacg acgcgctccg
     9781 agggcgggct ggggctggga ctcgccatcg tgcgccagat cgtcgaactg cacggcggca
     9841 cggtggaggc gaggagcccg gggctgggcg gcggcacgac gatgatcgtg aacctccctc
     9901 ccgccgaggc gcgaggcctc gcggcggtgc gcaccacggg cggatccacg gcgaagccgg
     9961 tggtctcgga aacgcccagc ctgagccgtc tccggcttct ggtcgtggag gacgatcccg
    10021 atgccctgga ggcgatctcc gccgtgctcc agcgatcggg ggcgagcgtg acgtcggtgc
    10081 gctcggtccc cgaagcgttg atcgcgctgg gccgtgagaa gctcgacgtc ctgatcagcg
    10141 acatcgcgat gtcgggcatg gacgggatca ccctgatccg cgccgtgcgc gagcggagcc
    10201 gcgagcgcgg aggagccatc ccggcgctcg cgctcaccgc ctacacgggc ggcgacacgc
    10261 agcggcgcgc gctggaggcg ggataccagg cctatctcac gaagccgatc gacgcgagcg
    10321 atctggtctc ggccatcgcc tcgctggcgg gcgtcgagcg gccggcgggg tgaccgcagg
    10381 gccgctccgc acctccgact tcgactacca ccttcccccc gaccggatcg cgcagcaccc
    10441 ttcggccgag cgcgacgaat cgcggcttct gatcctgccg cgtcgggcgg gtccgaacgc
    10501 gccggggtcg agccccgcgc cggggtcgag ccccgcgcct gagtcgaacc tcgcgccggc
    10561 gatcgagcac cgccgcttcc gcgaccttcc ctcctacctc cgtccgggcg acctcctcgt
    10621 cgtcaacgac acgcgcgtca tcccggcgcg gctcttcgcg cggctgccgc gcgaggccgg
    10681 ctgcgcttca ccccgggagg ccgagctgct tctggtccgg gaagagccgc cctttccggg
    10741 ggcgccgtcc gagcggttgt ggctggccct ggcccgcccc gcgcgacggc tgcgtcccgg
    10801 tctcccgctc gcgttcgccg atccttcctg gagcgcggtc gtatccggcg tcgaagagcg
    10861 cggcgcccgc cgcgtggcgt tccggcccca gggcacgctt tcgttcgaaa gctggctcgc
    10921 gcgcgtggga cacgtgccca tccctcccta catcgagcgg gcggacgagc ccgaggaccg
    10981 cgagcgttat cagacggtct atgcgcgcca ccccggctcc gtcgccgcgc cgacggcggg
    11041 gcttcacttc accgaggcga cgctccaggc ctgccgcgac cgcggcgtga cgatcgcgtc
    11101 ggtgacgctg cacgtgggcc ccgggacgtt ccgtccggtc gcgaccgag
//
LOCUS       DASSLZ010000100        21552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1302273, whole genome shotgun sequence.
ACCESSION   DASSLZ010000100 DASSLZ010000000
VERSION     DASSLZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 21552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21552
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302273"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFM00_03715"
     CDS             <1..782
                     /locus_tag="VFM00_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7497383.1"
                     /translation="GAYDPATGVLTHDPGAAVYEAELGISPALAGALGLPHGPSFHFA
                     LNDSLYKDNRIPPQGFTNAAFAAFGGAPVDPDRPALVPRYADGQNADTQRFGLPSTAR
                     SVAVELLYQTTSKEYVEFLRDQNTTNTAGGTMYNVWTGNGRSWPVVMQTDSVGLNITG
                     VPEQPSVAAAPALRVLRNPFGSALELRVDLARPTSVRLDVFNVQGRKVRTVDWGVLAA
                     GTHPLSWDGRLERGTSAGAGIYWISVRAGEHKFVRQVVRVK"
     gene            complement(832..1449)
                     /locus_tag="VFM00_03720"
     CDS             complement(832..1449)
                     /locus_tag="VFM00_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497384.1"
                     /translation="MTARRWVEPILRAPLTALLDLAVLAIALFNGSARGELVPFAAQA
                     WGFDPFQIDLARWPTLVTGVFLVRNPPMLVGILVFLVLSVGVYEARFGTGRAAALFGL
                     CHVVTLLATTALLVYPMHFAGMPRLSDWAPAGDVGASFGGFGCLGGWMRRLDRPARFR
                     WIAVVTIALAAKLVIYPERFGDIGHAIAFFAGMLLDRLLFSRRTA"
     gene            complement(1446..3449)
                     /locus_tag="VFM00_03725"
     CDS             complement(1446..3449)
                     /locus_tag="VFM00_03725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7497385.1"
                     /translation="MKLLLMVLGAGLALASVAAAGTPGPAAPPPLDLPLTRTPLEPPL
                     VVSGGFGEYRIGHFHAGFDLSTNRRVGKPVHAPESGWVERVRCSGVGYGRSVYLRAAD
                     GRTFQLGHLDAFAPAIDAYVRQAQAINGQYEQDLWPQPNEMRVRAGDVVAWSGESGAG
                     GPHLHFEIRRGDVAYHPQRAVLVVADRNPPSIPRLTLEPLDDSSAVRGVSGPITIPLG
                     PQADTVRAIGRLRVIVDARDGLWNGVDRMAPWSAGITWRGRTTECRFDSVSWATDMPE
                     GDYVHDAGRVTGGKGLVLWAPAGYRPRFLRSDAPLAEEAGTLRLAPGDAPETLAVFAR
                     DLGGNEVRRAIVLSPGAAPPPARPPFWKGDSSWAEVPGDFASLPGGRVRWSLPAGVAP
                     QGIELQIGTTSRRATRAGEEWCATFPYPGLRRGRAVRVPLAIRGKSGSRLDSGGMVTG
                     AGLDVSDSLELSLDGNRLFLPAGAVFEGGFVFAARSRGGRPARGLERVPGAWSVEPST
                     MPLRLPARVTLTVPAGERLDRVGLCRLDADGWSWVGAQRDSVRRTVTAETGRLGTFAL
                     FRDTTAPRVALQRAPKSAATKPYSRWALEAAVTEEGSGVDARGSWLEVDGARVPTEWD
                     PEAGRLRWRPARPPRRGAHQVLVVASDRAGNVAKTRGASRVGR"
     gene            3537..4304
                     /locus_tag="VFM00_03730"
     CDS             3537..4304
                     /locus_tag="VFM00_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497386.1"
                     /translation="MNTRTLRRATLPAALTVAFLALSPHTAAAQAAPGDSIPAARLEP
                     LPFAKNSLPPPPLGFGVGIAVPTQGLGGVERAFEKIENVYRSQGYSLPSPHLSLDAMM
                     TYRMTVRPGKSYEVVGQLCRSRGNESGTESELNTTGLLVARRFASTESGAYSLLVGLG
                     GGAYGFLFRREYNAQVSPTNADGSFYLLRHITLQGGGAYGTGAAGITLRAGRHVAFDG
                     LAQYLWMKDTAKSLSPAGEVSFNLSGLALGGTLSFFY"
     gene            4334..5884
                     /locus_tag="VFM00_03735"
     CDS             4334..5884
                     /locus_tag="VFM00_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497387.1"
                     /translation="MLRTIGRAVLILAVTALTGCGTSRTLTTRGQLMAAGTTPITVYS
                     EDRHVYTLRQHVVEDSVITGAGTVRRGDERSAFQGSIPLTSIRAVKTNSKNVMKGLAL
                     LGVTALFVEALAEGNGHSGGLRALEDKPLVYPYSGGGGGESCPYVYSWNGERYVLEAE
                     PFGVALGKGLELTTTHLLPSARAEKRVIRVRMTNERRETHYVNSLSLYAIDLGDAPAA
                     VLDGRGIAWPLRHAMAPVAAHDAQGHSILGAVARTDGRMWECDASRLLPGSGYEDVVE
                     LAFAVPRSAAEGTLVLTGINTSLSTAIYGHLVRAAGDPVTIAHAIDGDPELIARLRDY
                     LADASLKASVWNGREWEPAGSFQPEANAVTFTRALRIRLPGGAGDTVRVRLRSMADVW
                     KIDALAADWTEAAPLTMEPMTLLRAAGPDGEDLSDALRGDDDRYAILVPPDRVELAYE
                     ARSRAPGNRVVYAVAGRGYLHEWTPEGVAENASWAPDEGRLALLKELLKHRDLALRPV
                     YEEWRSAR"
     gene            complement(5898..7166)
                     /locus_tag="VFM00_03740"
     CDS             complement(5898..7166)
                     /locus_tag="VFM00_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7497388.1"
                     /translation="MTTPGQLRRVVFASFVGTTIEWYDFFLYGTAAALVFNRLFFPTL
                     DPLAGTLSAYGTFAVGFVARPLGGAVFGHYGDRIGRKTMLIWSLVIMGVASALIGLLP
                     GYDRIGIWAPSLLVVLRFLQGFGVGGEWGGAVLLAVEHSGSERRGFHGSWPQMGVPAG
                     LLLSTGLVTALSARLPERAFLDWGWRVPFLLSVVLVGIGLFVRLRVLESPAFAAVREA
                     GRESRAPLLDVFRRHPREVVIGAGMRFAQNVLFYIYTVFVLSYGEKTLGYPRSAVLGG
                     VVLSAVVGLVAIPFWSHLSDRVGRRPVYLAGAALSLVTAFPFFWLFGKGPGFVAAAIV
                     LAMVGHDMMYGPMAAYFSELFPTSVRYSGSSLVYQFTSVFSGGLAPLVATLLLSRHGP
                     GAVATYLVACCAITLAATWFAPETHRAGLE"
     gene            complement(7168..7671)
                     /locus_tag="VFM00_03745"
     CDS             complement(7168..7671)
                     /locus_tag="VFM00_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497389.1"
                     /translation="MKRGSAWAWVRVLICAVSLAGCSPGGASRTALVGAWRSSVQFDT
                     GAFATIKDLEFMYVFHDDGTLTESSNYDAAPPVPPAYGVWRTIRPNEFEARYEFYATS
                     PSAPDAFKTGAGWLPSGRGVLTERIRLSDDGASFTSTIQYQALDRAGKPAEGGGTAKG
                     RGAKMRL"
     gene            8015..8419
                     /locus_tag="VFM00_03750"
     CDS             8015..8419
                     /locus_tag="VFM00_03750"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HET7497390.1"
                     /translation="MNHKMTDLKGIDQDQVAKLKAAGVETTSEMMKVWHDPDQRAKLA
                     TTSGLSEEQFKRMVSLARMARMPGVGPKYAELLVTSGVIGRKSLSKHTPEGLVKHLAE
                     VSASRKLTGPVPTLTEVGAWFAELKPLVGPPE"
     gene            8556..10493
                     /locus_tag="VFM00_03755"
     CDS             8556..10493
                     /locus_tag="VFM00_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HET7497391.1"
                     /translation="MTIRGLVLGALITTVFTAANVYLGLKVGLTFASSIPAAVISMAI
                     LSAVKDSSILENNIVQTVASAAGTLSAIIFVLPGLVIVGWWTGFPFWQSFLICVSGGV
                     LGVLFTIPLRRALVTTSDLPYPEGVAAAEVLRVGSGTRGETKDDTGEAREGLVAVILG
                     SVASAGLAIVTATRIAAAEVTGFFRVGARASSGYDVAWSLALLGAGHLVGLSVGMAML
                     TGLIIAWGIAVPILTSMQPAAAGIGLDAHTLTIWRTQVRFIGAGAIAVAAIYTLATLA
                     KPVIGGLVGTLAASRAKASSDERDRDLSPGWIVVLTAACLVVAGWLAFTFAKSTALAP
                     SAVPLTVIAIPFVLIGGFLIAAICGYMAGLIGASNSPISGVGILSIVLCASAIIVAVV
                     PTPETRGPLVAFALFVTAIVFACATISNDNLQDLKTGQLVGASPRRQQIALIVGVAAG
                     ASVIPWVLNLLAKAYGFAGAPNVGVVAPNPLPAPQATLISALAQGVIGGNLDWKMLGI
                     GALVGVGLILMDTTLGAMKKLRIPPLAVGIGIYLPMSATFAVVVGAVVGHWYDRRART
                     MPNPGRSERLGTLVASGLIVGESLWGVINAGLIVAFAKDAPIGLVPETFALAPWLGLL
                     GFVGMIVWLYGWMLRRSAEAR"
     gene            complement(10528..11616)
                     /gene="rho"
                     /locus_tag="VFM00_03760"
     CDS             complement(10528..11616)
                     /gene="rho"
                     /locus_tag="VFM00_03760"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET7497392.1"
                     /translation="MSGYLEVLERGGGFLRDPARSFAPSGEDVYVSQGIITRYQLISG
                     AAVTGAAVEGGQGPRLESVATICGLPPERFRERSSFTSLTAIDPDRRFPLGILGNLSM
                     RIIDLFAPIGRGTRGLIVSPPKAGKTTLLEEIAAAILAGAPNTIVTVLLIDERPEEVT
                     QFRRSVAAEVFASSSDESVEDHVRLATMCMAKARCELMCGRDVVVLVDSLTRMGRAFN
                     AYGSDSGRTMSGGLDSRALEIPRKFFGMARNVEGGGSITVLATALIETGSRMDDLIFE
                     EFKGTGNSEVVLDRALSDQRVFPAINIAKSGTRKEERLYSKDEIEAINRLRRRLLELP
                     PKQAILELRKVMEQFPTNEELLKQIPGS"
     gene            12158..12358
                     /locus_tag="VFM00_03765"
     CDS             12158..12358
                     /locus_tag="VFM00_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7497393.1"
                     /translation="MAAGTVKWFNESKGFGFIQQEGGEDVFVHYSAISGEGFKTLAEG
                     ERLEFDVVRGPKGLQAANVRKA"
     gene            complement(12431..14680)
                     /locus_tag="VFM00_03770"
     CDS             complement(12431..14680)
                     /locus_tag="VFM00_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7497394.1"
                     /translation="MIGRKLSHYQVLERLGAGGMGEVYRAHDDRLERDVAVKVLPNIA
                     LANEDARRQFRREALALSRLNHPHIATVYDFDTDQGVDFLVMELVLGKSLADHLKREP
                     LPEAEIVRLGSQIAEALEAAHEQGVIHRDLKPSNIMVTPKGQTKVVDFGLARLQEKGG
                     QTLATASSGIQGVAGTLPYMAPEQLASGVIDARTDLYALGAVLYEMTTGRRPFEKDRI
                     EALMFEILHGVPLPARSIREEISQELDRLIEACLSKDAGQRPASAVAVIQTLRHIGQP
                     GSRGGNGESVREALPSLAVLPFENVSRDPAQEYFADGMTEALISELARLKALRVISRT
                     SAMRYKGSQKALPEIARELNVSAILEGSALLVGKRVRVSAQLISARSDETLWADRYDG
                     DLEDVLDLQSRVAEAVAKEIQVHVTPKEAKRLAKRQAAVNPEAHVEYLKGRHTAAATS
                     PQAIELSLRYYDRALKLDPTFAPTWAGIAHSHVVRAERGMAAPAEAAPLARSAALKAL
                     ELDDSLADAYSALGEVAILDRDLPAAIRNLEHAIDLNPEPGAYMALGSIYYCTERHRE
                     AQEAMLQALRLDPVSMIIHTAVGDAYYYAREYERSLVYYQKAVEIDPRFDGAHTDLAR
                     SLEAVGRFEDSRREYEEGRRLSGGVAGPHFGVAHLEISMGNTEEARRQLEELIAARSR
                     RVVSAWGIAALHARLGDVDEAFRWLDVAVEEKATGLIFLRVHPRLDGIRQDPRYGALV
                     RKLKLDTAG"
     gene            14775..16112
                     /locus_tag="VFM00_03775"
     CDS             14775..16112
                     /locus_tag="VFM00_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7497395.1"
                     /translation="MAGLCTAAASWSLPSGSTGAPLAFPSSDAPGDTVTIHDCVAMAR
                     EHAPAILAAGSDLLAAAYESTAVARERGPDWAITSDATVAPRGFYDPTVTDLGGYDLK
                     LGFDWPLADGGKRFLARRRSALDLAQARWQSMQTARDVGLRAGELAAELLRLQEIAAA
                     QGGAVEWTVSLGALVKRGVTSGVRSAADSIRVGLERDAALLSLESTLQEARTDELELL
                     VLLGMDPLGHVVIAPAPGSDRPPGEDDSLRVVASADRAPEVGLAEITAARGSLDLLEA
                     RRRNAATIDLAADAGLAGADLTSAVPEDLKAANPDATLGDRLRRDLGASVAFHFRMPV
                     LDKSAARHARAREETLRAARAKALAERGTQRRVALDLLSQWRFAYRRAELAQRSRDRA
                     EDALLRVKSLYAGGATTLLDLLDARRVTEEARERLAEARRDARLAQLRVEDRE"
     gene            16109..17095
                     /locus_tag="VFM00_03780"
     CDS             16109..17095
                     /locus_tag="VFM00_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET7497396.1"
                     /translation="MTRWRIARAGGTVVAALLAAAAVGGCSKHEESDTDETTARVPVR
                     AARVETRTIQSQIAATGQWRAADSVVVIAPFAAYVESVRPHLGDRVAKGQTIAGLVTH
                     ESRAALRGAEILVRQASGAAESQEARRALAQAERGIVRVPLIASVAGTVIRRSAEPGS
                     EVAESAPLLTLVPQGSFVFEAHVAHADARHVRPGQAATIRLEAGDVIPAVIQRLLPQA
                     SESDQNQLVWLSPGRVPTGALDRFGTASIETGSVRSAAAVPDSALVEDDLTGQVRIAR
                     IVGGIAIWTPVRLGQGNGGWHEILAPALPPGTPVVISGQRGLPDSTKVEIAR"
     gene            17092..20148
                     /locus_tag="VFM00_03785"
     CDS             17092..20148
                     /locus_tag="VFM00_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7497397.1"
                     /translation="MTGFFDTVHRQRRALSLLLASLTILSFAAVRKLPTSILPEVTFP
                     RIKIVADSGELPSDAMLRSVTRPLEESLRRVPGVSEIRSTTSRGSTEINLDSRWNADM
                     NLTLQRVQAQMEAVRPELPAGTTLDARLMNPTLFPVLGFSLTSDRVSSAQLRDFATLI
                     LKPELSRLPGVAEVVLQGGHKLEARITLDPVALEARGLTTQGLVEAIRKAAVLEPVGL
                     LEANRQLYLGLVDSRPTDLGTLATLPIPLASGAPVALGQLGKITLEEAPEFVRYRARQ
                     REAVLVNVLRQPSASAIALSQEAHRWLTEHARQLPPGTHVETFYDQSDLVRASVGSVR
                     DSLLVGAILAFLVIVFFLRSVRLALAGAAVLFFSIALTLVGLMLAGQSLNMMTLGGIA
                     AAVGLVLDDGIVVVEHLAARTAGPSTVSRSQAMAEIFPSLIGSSLCSLAIFLPFIFLG
                     GVTGAFFRVLALSMSLMLGFSLLLCLTLVPLLSPTGHAERPPRTPGRTTERLRLLLDR
                     SIRRPRMAALAVALLIVLAVPLYATLGTGFLPEMDEGSLILDYVSPPGTSLTETDRML
                     QEVERIIAGMPEIASWSRRTGDQLGFFITEPNTGDYVLRLRSGHRRSADAIADDLRQR
                     IESSQPALEVEFGQLVEDVIGDLTTNPQPIEVRVFSEDRAIAESKASEIATLLERVRG
                     VVDVKSGVVVSGPTLVASPNATAAHAALDAGDMAAAAAPAVAGVDVGEIVRGARAWSV
                     RVVLPRPSGLSGAEILARVPVSVGTGAHARLGDLAAVATNPGETEIAHDNLRTMVAVT
                     ARLSGRDLGSAIAEIQGRVRRELVLPREASVQYAGLWAEQQSSFRGLTGVALAAAALV
                     ALILLASFQAWPEMIAVLLVVLASLAGVFTALRLSGATFNISSFVGVIMMVGIVSENA
                     YFLVAAFQARLVSGDSPPDAARAAALRRARPVLMTTFAGIAALAPLALGLGAGSALLK
                     PLAVAVVGGFSISAFLLLLVLPALLGRFASPSLPQPRARNDGALPT"
     gene            20166..20459
                     /locus_tag="VFM00_03790"
     CDS             20166..20459
                     /locus_tag="VFM00_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497398.1"
                     /translation="MTERFVLSSFYGYFAAFVLVEFVLTRFRLGTGERGNRDRLSALF
                     FLFVPFAGIFLWIFCVNAHLVGARSRSSWACSSASLAKRRSWSKGWARAIAPT"
     gene            complement(20528..>21552)
                     /locus_tag="VFM00_03795"
     CDS             complement(20528..>21552)
                     /locus_tag="VFM00_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7497399.1"
                     /translation="ENGQKRMAGMKARDQAGALPDDFQQMLRAFQESRTLLTAIELDL
                     FTAVGAGADAAAVARSIDTDPRATGMLLNALAAMDLLTKRDGEFFNGPVAGRYLVAGG
                     PDDSRAALLHTVHLWSRWSTLTECVRQGTSVTLGDRRGADWVETFIAAMHKNAAFRAP
                     LVARAIGLDGVKRVLDVGGGSGAYAIAFARSRPGLEVEVLDLPDVVPIAQRYIEAAGV
                     GSQVRVRTGDLRDPSFGTGYDLVLFSAICHRNTSEENQAMLGKAFAALAPGGRVVIHD
                     FILNADKGGPKTGALFALNMLVGTRAGDSYSGEEYAAWLRDAGFAEVRTVGLPGPTGL
                     VIGKRP"
BASE COUNT         3216 a         7615 c         7437 g         3284 t
ORIGIN      
        1 cgggagcgta cgaccccgcc acgggcgttc tgacccacga ccctggagcg gcggtctacg
       61 aggccgagct gggcatctcg ccggcgctcg ccggtgctct gggcctgccg cacggcccgt
      121 cgttccactt cgcgctcaac gattcgctct acaaggacaa ccggattccg ccgcaggggt
      181 tcacgaacgc ggcgttcgcg gcgttcgggg gggcgccggt ggatcccgac cgtcccgcgc
      241 tcgttccccg gtacgcggac gggcagaacg cggacaccca gagattcgga ctgccgagca
      301 ccgcgcggtc ggtggccgtg gagcttctct atcagaccac gtcgaaggaa tacgtcgagt
      361 tcctgcgcga tcagaacacg accaacacgg cgggagggac gatgtacaac gtgtggaccg
      421 ggaacggccg atcgtggccg gtcgtgatgc agaccgattc ggtgggcctg aacatcaccg
      481 gcgtcccgga gcagccctcc gtggcggcgg ctcccgcgct tcgcgtccta cgcaacccgt
      541 tcggatccgc gctcgagctg cgcgtggatc tggcgcggcc cacgtccgtc cggctcgacg
      601 tcttcaacgt ccagggccgg aaggtccgga ccgtcgattg gggcgttctg gccgcgggca
      661 cccatccgct ctcgtgggat ggccgcctgg agcgcggcac gagcgcgggc gccgggatct
      721 actggatctc ggtccgcgcg ggtgagcaca agttcgtccg gcaggtcgtc cgcgtgaagt
      781 gatctctcgc gggttcggac gtgagacccc gggagcctcg cttccggggt ctcaggctgt
      841 ccggcgcgag aacaggagcc ggtccaggag catccccgca aagaacgcga tcgcgtgccc
      901 gatgtcgccg aaccgctcgg ggtagatcac gagcttcgcc gcgagcgcga tcgtgacgac
      961 ggcgatccat cggaaccgcg cgggccgatc gagccggcgc atccaccccc ccaggcatcc
     1021 gaaccctccg aacgaggcgc cgacgtcccc cgccggcgcc cagtccgaaa ggcggggcat
     1081 cccggcgaag tgcatcgggt agacgagcag ggcggtcgtg gcgagcaggg tcacgacgtg
     1141 gcagaggccg aacagggccg cggcccgtcc ggtgccgaag cgcgcttcgt agacccccac
     1201 cgacaggacg aggaacacga ggatccccac cagcatcggc ggattccgca ccaggaacac
     1261 tccggtgacc agcgtcggcc agcgggccag gtcgatctgg aagggatcga acccccatgc
     1321 ctgcgcggcg aaggggacca gctccccccg ggccgagccg ttgaagaggg cgatcgccag
     1381 gacggcgaga tcgaggagtg cggtcaacgg ggcgcggaga atcggctcga cccagcgccg
     1441 cgcggtcatc ggccgacccg ggacgcgccg cgcgtcttcg cgacgttccc cgcgcggtcg
     1501 gacgcgacga cgagcacctg gtgggcgccc cggcggggcg gacgcgccgg tcgccagcgc
     1561 aggcgccccg cttcgggatc ccattccgtg ggcacgcgcg ctccgtccac ttcgagccat
     1621 gacccgcgcg cgtcgacgcc gcttccctcc tccgtgaccg cggcctccag cgcccagcgg
     1681 ctgtagggct tcgtcgcggc gctcttggga gcgcgttgga gcgcgacgcg cggcgcggtc
     1741 gtgtcgcgga agagggcgaa ggttccgagt cggcccgtct ccgccgtgac ggtgcgccgc
     1801 acgctgtcac gctgcgctcc gacccatgac cagccgtccg catccagccg gcagaggccg
     1861 acgcgatcga gccgttctcc ggcgggaacc gtcagggtca cgcgcgccgg aaggcggagc
     1921 ggcatggtgg acggctccac gctccaggcg cccggaaccc gttcgagccc gcgcgccggc
     1981 cgtcccccgc ggctccgggc cgcgaatacg aagccccctt caaagacagc gcctgccgga
     2041 aggaagagcc ggtttccatc cagggacagc tcgagcgagt ccgagacatc cagcccggca
     2101 ccggtgacca tgccgccgga atcgagacgc gacccgctct tgccgcggat cgcgagcgga
     2161 acgcgaacgg cgcggccccg cctcaggccg ggatacggaa acgtggcgca ccactcctcc
     2221 cccgctcgcg tggcccttcg gctggtcgtg ccgatctgca gctcgattcc ctgcggcgcg
     2281 acgccggccg gcaggctcca gcgaacgcgt ccgccgggaa gggatgcgaa gtcgcccggc
     2341 acctcggccc acgaggaatc tcccttccag aatggtggtc tcgccggcgg gggggccgcg
     2401 ccgggactga ggacgatcgc gcggcgcacc tcgtttccgc cgaggtcgcg cgcgaagacc
     2461 gcgagcgttt ccggagcatc gccgggtgcc aggcgcagcg ttcccgcctc ttccgcgagg
     2521 ggagcgtccg agcgcaggaa tcgcggacgg tagcctgcgg gcgcccagag cacgagtccc
     2581 ttcccgccgg tcacgcgccc ggcgtcgtgg acgtagtccc cctccggcat gtcggtggcc
     2641 caggagacgc tgtcgaaccg gcattcggtc gtccggccgc gccacgtgat ccccgccgac
     2701 cacggcgcca tgcggtccac gccgttccag agaccgtcgc gcgcatccac gatcacgcga
     2761 agccgcccga tcgcgcgcac cgtgtccgcc tgcggcccga gcggaatggt gatcggaccg
     2821 gagacgcccc gaacggccga cgaatcgtcg agcggctcga gcgtgagtcg cgggatcgaa
     2881 ggcgggttcc gatcggccac cacgaggacc gcgcgctgcg gatggtaggc cacgtcgccg
     2941 cgacggatct cgaaatggag atgcggcccg ccggcgccgc tctctcccga ccaggcgacg
     3001 acgtctccgg cgcgcacgcg catctcgttg ggctggggcc agaggtcctg ctcgtactgg
     3061 ccgttgatgg cctgggcctg acggacgtag gcgtcgatcg ccggcgcgaa cgcgtcgaga
     3121 tggccgagct ggaacgtgcg gccgtccgcg gcccgcaggt acaccgaccg gccgtagccc
     3181 actccggagc agcggacgcg ctccacccag ccgctctcgg gagcgtgcac cggtttcccg
     3241 acgcggcggt tggtcgagag atcgaagccc gcgtggaagt ggccgatccg gtattcgccg
     3301 aatcctccgc tgacgacgag cggcggctcc agcggtgtgc gcgtcagggg tagatccagc
     3361 ggcgggggcg cggcgggtcc cggcgtgccg gcggcggcga cactcgcaag cgcaagcccg
     3421 gcaccgagaa ccatgagcag aagcttcatc accggatact aacccgatcc cccgccgttc
     3481 gcttcggcca atggcctgcg ttcatgggtt caggagttca ggaaaggggg tcggacatga
     3541 acacgcgcac gcttcgacga gctacgctgc cggcagcact gacggttgcc ttcctggcac
     3601 tgtcgcccca cacggccgcc gcccaggcgg ctccgggaga cagcatcccg gcggcgcgcc
     3661 tcgagccgct cccgttcgcg aagaactcgc taccgccgcc ccctttgggc ttcggcgtcg
     3721 gcatcgccgt ccccacgcag ggtctcggcg gcgtggagcg agccttcgag aagatcgaga
     3781 acgtctaccg ctcgcagggc tattcgcttc ccagcccgca cctcagcctg gacgcgatga
     3841 tgacgtaccg catgacggtg cggcccggga agagctacga ggtcgtggga cagctctgcc
     3901 gctcgagggg gaacgagtcc ggcaccgaga gcgagctcaa cacgaccggg ctgctggtcg
     3961 cgcgtcggtt cgcgtccacg gagagcggcg cctattcgct gctcgtcgga ctgggcggcg
     4021 gagcctacgg attcctcttc cgccgggagt acaacgctca ggtgtctccg accaacgccg
     4081 acggcagctt ctacctcctg aggcacatca cgctccaggg cgggggagcg tacggcaccg
     4141 gagccgccgg catcacgctt cgagccggcc ggcacgtcgc gttcgacggt ctggcccagt
     4201 acctgtggat gaaggacacg gcgaagagcc tatcgcccgc cggcgaggtc tcgttcaatt
     4261 tgagcgggct ggctctgggc ggaacgctct cgttcttcta ctgatgacgc cgtaccgaag
     4321 ggagggatcg tccatgttgc gaaccatcgg gcgcgccgta ctgatcctgg cggtgaccgc
     4381 gctcacggga tgcggcacct cgcgaacact cacgacccgc ggccagctca tggcggcagg
     4441 gaccacgccg atcaccgtct actcggagga tcgtcacgtc tacacgctgc gccagcacgt
     4501 cgtcgaggat tccgtgatca ccggggccgg caccgttcgc cgcggggacg agcgttcggc
     4561 gttccagggg tcgatcccgc tcacgtccat tcgagccgtg aagacgaact ccaagaacgt
     4621 catgaagggg ctggcgctgc tcggcgtgac ggcactcttc gtcgaagccc tggccgaggg
     4681 gaacggtcac tccgggggac tccgggccct cgaggacaag cctctcgtct atccctactc
     4741 cggaggaggg ggcggggagt cgtgtccgta cgtctattcc tggaacggcg aacgctacgt
     4801 gctggaggcg gagcccttcg gcgtggcgct gggcaagggg ctggagctga cgacgaccca
     4861 tctcctcccc tccgcgcgcg ccgagaagcg cgtgatccgg gtccggatga cgaacgagcg
     4921 ccgggagaca cactacgtca attcgctctc gctctacgcg atcgatctcg gcgacgctcc
     4981 ggccgcggtg ctggacggcc gcggaatcgc gtggccgctg cggcatgcga tggccccggt
     5041 cgccgcgcat gacgcccagg gacacagcat cctgggcgcg gtggctcgca cggacgggcg
     5101 catgtgggag tgcgacgcct cccgcctcct gcctggatcc ggctacgagg acgtcgtcga
     5161 gctggcgttc gccgtgccgc ggtccgccgc cgagggaacg ctggttctga ccggaatcaa
     5221 cacctccctc tccacggcga tctacggaca cctggtgcgc gcggccggcg atccggtgac
     5281 gatcgcgcac gcgatcgacg gcgatcccga actgatcgct cgccttcgcg actatctggc
     5341 cgacgcctcg ctcaaggcct cggtgtggaa cgggcgcgaa tgggagccgg cgggatcgtt
     5401 tcaaccggag gccaacgccg tgacgttcac ccgcgcgctt cgcatccgcc tccccggcgg
     5461 cgccggagac accgtacgcg ttcgtctcag gagcatggcc gacgtgtgga agatcgacgc
     5521 gctcgcggcg gactggaccg aagccgcgcc gctcacgatg gagccgatga cgctgctgcg
     5581 cgcggcgggg cccgacggcg aggacctctc ggacgcgctc cgcggggacg acgatcggta
     5641 cgccatcctg gttccgcccg atcgcgtgga gctagcgtac gaggcgaggt cgcgggcgcc
     5701 cggaaacagg gtcgtctacg cggtggccgg ccgcgggtat ctccacgagt ggaccccgga
     5761 gggagtcgct gagaacgctt cgtgggcgcc cgacgagggg aggctggcgc tgctcaagga
     5821 attgctgaag caccgcgacc tggcgctccg gccggtgtac gaggagtggc ggtcggcgcg
     5881 ctgaagacgg cggggagcta ctcgaggccc gcccggtggg tttccggcgc gaaccaggtg
     5941 gccgcgagcg tgatggcgca gcaggcgacg aggtacgtcg ccaccgcgcc gggtccgtga
     6001 cggctcagga gcagcgtcgc caccaggggc gccagcccgc ccgagaacac cgacgtgaac
     6061 tggtagacga gcgagctgcc gctgtagcgc accgaagtcg gaaagagttc cgagaaatac
     6121 gccgccatcg gaccgtacat catgtcgtgg ccgaccatcg cgagcacgat ggcggccgcg
     6181 acgaatccgg gccctttgcc gaacagccag aagaagggaa acgcggtcac gagcgagagc
     6241 gctgcacccg cgaggtacac ggggcgccgc ccgacgcgat ccgagagatg cgaccagaac
     6301 ggaatggcga ccagtcccac cacggccgac agcaccaccc cgcccagcac ggcgctgcgc
     6361 gggtagccga gcgtcttctc gccgtagctc agcacgaaca ccgtatagat gtagaagagc
     6421 acgttctgcg cgaaacgcat ccccgctccg atcaccacct cgcgcggatg acggcggaac
     6481 acgtccagga ggggtgcccg cgactcgcgg ccggcctcgc ggaccgccgc gaacgcgggc
     6541 gactccagca cgcgtaggcg cacgaagagg ccgatcccga cgagcacgac cgagagcagg
     6601 aacggcacgc gccatcccca gtccaggaac gcacgctccg gaaggcgggc cgaaagcgcg
     6661 gtgacgagtc ccgtcgaaag caggaggccg gccggaacgc ccatctgggg ccaggatcca
     6721 tgaaagccgc ggcgctcgga gcccgagtgt tccaccgcca gcagcaccgc tcctccccac
     6781 tcgccgccga cgccaaaacc ctgcaggaat cgaagcacga cgaggagcga cggcgcccag
     6841 atgccgatcc ggtcgtatcc ggggagcagc ccgatcagcg ccgaggcgac gcccatgatc
     6901 acgagcgacc agatcagcat cgtcttccgc ccgatccggt cgccgtagtg gccgaagacc
     6961 gctccgccga gaggacgcgc gacgaatccg accgcgaagg ttccataggc gctcagggtt
     7021 cccgcgagcg gatcgagcgt ggggaagaag agccggttga agaccagcgc cgcggcggtg
     7081 ccgtagagga agaagtcgta ccactcgatc gtggtgccga cgaaggaggc gaagacgacg
     7141 cgccgaagct gacccggagt ggtcacgcta gagcctcatc ttcgccccgc gccccttggc
     7201 cgttccgccg ccctcggccg gctttccggc gcgatccagc gcctggtact ggatcgtcga
     7261 cgtgaaggag gcgccgtcgt ccgaaagccg gatccgctcg gtcagcacgc cgcgcccgga
     7321 agggagccaa cccgcgccgg tcttgaaggc gtcgggtgcc gagggcgacg tggcgtagaa
     7381 ctcgtacctc gcctcgaact cgttcggacg aatcgtgcgc cacacgccgt aggcgggcgg
     7441 aacgggcggc gccgcgtcgt agttcgagga ctcggtcagc gtgccgtcgt cgtgaaagac
     7501 gtacatgaat tcgaggtcct tgatcgtcgc gaaggccccc gtgtcgaact ggaccgaaga
     7561 gcgccaggcg ccgacgagcg cggtccgcga agccccgcca gggctgcatc cagccaacga
     7621 gaccgcgcag atcagtaccc gtacccatgc ccaagccgat cctcgtttca tctcgacctc
     7681 cgacgtgcac gtcgatcgca ccggccgccg ccgccgcctg ggaatccctt gctccttacc
     7741 tccaatcggt ccagaataca tccatagcct cttgcttcaa ggccacccct gacggtggcc
     7801 gaccccgtgg cggcggaccg cccgcgtcac gggtccaaac ggcgcaggcc tgcggcttcg
     7861 gtacccacgt acttcagccg cccgaaatcg aaccggccgc ccgtaccctc ctcgaaccgg
     7921 tcccagaacg gcatctctcg agaaccaggc ctcagggcca tacggctcga ccgagccagc
     7981 gtgcgcatgc acagggcgcc gggaaggaat ggacatgaac cacaagatga cggatctgaa
     8041 aggcatcgat caggaccagg tcgccaagct gaaggcggcc ggggttgaaa ccacgagcga
     8101 gatgatgaag gtctggcacg acccggatca gcgcgccaag ctcgcgacca cgtccggtct
     8161 cagcgaagag cagttcaagc ggatggtctc gctggcgcgg atggcgcgca tgccgggcgt
     8221 ggggccgaag tacgcggagc tcctggtcac gtccggcgtc atcggccgga agagcctctc
     8281 caagcacacc cccgaagggc tcgtgaagca tctggcggaa gtgagcgctt cgcggaagct
     8341 gaccgggccc gtgcctacgc tgacggaagt cggcgcctgg ttcgccgagc tgaagccgct
     8401 cgtcggcccg ccggaatgat ccgcgtctga aacgccgcct cggggccccg gcggccgaac
     8461 cgccggggca tttcctgggg agggcctccc cctgattcca gggtagcccc gcctcccgcc
     8521 cgctgctatc gtcacggcgc ttgaaaccca gagagttgac gatacgcggc ctcgtcctgg
     8581 gcgcgctgat caccacggtc ttcacggccg cgaacgtcta tctcggcctg aaggtcgggc
     8641 tcaccttcgc ttcgtccatc cccgcggccg tcatctcgat ggccatcctg agcgccgtca
     8701 aggactcgtc catcctcgag aacaacatcg tgcagaccgt cgcgtccgcg gccggcacgc
     8761 tctcggcgat catcttcgtc ctgcccggcc tggtgatcgt gggctggtgg accggatttc
     8821 ccttctggca gtcgttcctg atctgcgtga gcggcggcgt gctgggcgtg ctgttcacca
     8881 ttcccctccg gcgcgcgctg gtgaccacgt ccgaccttcc ctaccccgag ggggtcgcgg
     8941 cggccgaggt gctgcgggtc ggctccggca cgcgcggcga gacgaaggac gacaccggcg
     9001 aggcgcgcga ggggctggtc gccgtcatcc tcggctccgt cgcctcggcg gggctcgcca
     9061 tcgtgacggc gacgcgcatc gccgccgccg aggtgaccgg cttcttccgt gtcggcgccc
     9121 gggcctccag cggctacgac gtcgcgtggt cgctcgcgct gctcggcgcg gggcatctcg
     9181 tggggctttc ggtcgggatg gccatgctga ccggcctcat catcgcctgg ggcatcgccg
     9241 tcccgatcct cacctccatg caacccgccg ccgccggcat cgggctcgac gcgcacacgc
     9301 tcacgatctg gcgcacgcag gtgcgcttca tcggggccgg cgccatcgcc gtggccgcga
     9361 tctacacgct ggccacgctg gcgaagccgg tcatcggcgg cctcgtcggc acgctcgccg
     9421 cttcccgggc caaagcctcc tcggacgagc gggatcgcga cctctcgccc ggctggatcg
     9481 tggtgctcac ggccgcatgc ctcgtcgtcg ccggctggct cgccttcacg ttcgcgaagt
     9541 ccacggcgct cgcgccgagc gcggtgccgc tcaccgtgat cgccatcccc ttcgtgctga
     9601 tcgggggctt tctgatcgcg gccatctgcg gctacatggc ggggctgatc ggtgcctcga
     9661 acagcccgat ctcgggggtg gggatcctct cgatcgtgct ctgcgcgtcg gcgatcatcg
     9721 tcgccgtggt gccgacgccc gagacgcgcg gccccctggt cgcgttcgca ctcttcgtga
     9781 cggcaatcgt gttcgcctgc gccacgatct ccaacgacaa tctccaagac ctgaaaacgg
     9841 gccagctggt cggcgcctct ccccggcgcc agcagatcgc gctgatcgtg ggggtggccg
     9901 cgggcgcctc cgtgatcccc tgggtgctca acctgctggc caaggcctat ggattcgccg
     9961 gcgcgccgaa cgtcggcgtc gtcgcgccca atcccctgcc cgcgccccag gcgacgctca
    10021 tctccgcgct cgcccagggc gtgatcggcg ggaacctcga ctggaagatg ctcggcatcg
    10081 gcgcgctcgt cggcgtggga ctcatcctga tggacacgac cctcggcgca atgaagaagc
    10141 tgcgcattcc gccgctggcc gtgggcatcg ggatctacct tccgatgtcg gccaccttcg
    10201 ccgtcgtcgt cggcgccgtg gtaggacact ggtacgatcg acgcgcccgc accatgccga
    10261 accccgggcg ctccgagcgg ctgggcacgc tggtcgcgtc gggtttgatc gtgggcgaga
    10321 gcctgtgggg cgtgatcaac gccgggctga tcgtcgcctt cgccaaggac gcgccgatcg
    10381 gtctcgttcc cgagaccttc gcgctcgcgc cgtggctcgg gcttctcgga ttcgtcggca
    10441 tgatcgtgtg gctctacggc tggatgctga ggcgttccgc agaagcccgc tgagcggtcc
    10501 cgcgcgggca accccgtgcg cgacggacta ggagcccggg atctgcttca gcagctcctc
    10561 gttcgtggga aactgctcca tcaccttccg caactctaat attgcctgct tcggcggtaa
    10621 ttccaggagc ctgcggcgca gccggttgat cgcctcgatc tcgtccttgg agtagagccg
    10681 ttcttccttt cgcgtcccgc tcttcgcgat gttgatggcc gggaacacgc gctggtcgga
    10741 cagcgcgcgg tcgagcacga cctcgctgtt tccagtcccc ttgaattcct cgaagatcag
    10801 gtcgtccatg cgcgagccgg tctcgatcag cgcggtggcc agaaccgtga tcgatccgcc
    10861 cccttcgacg ttccgcgcca tgccgaagaa cttgcgcgga atttcgagcg cgcgcgagtc
    10921 cagcccgccg ctcatggtgc ggcccgaatc ggatccgtac gcgttgaagg cgcgccccat
    10981 ccgcgtgagg ctgtccacca gcacgacgac gtcgcgcccg cacatcagct cgcatcgcgc
    11041 cttggccatg cacatggtcg caaggcgcac gtgatcctcg accgactcgt cgctgctgct
    11101 cgcaaagacc tcggcggcta cggaacgccg gaattgggtc acctcttccg gccgctcgtc
    11161 gatgagcagc acggtgacga tcgtgttggg cgctcccgcg aggatcgcgg cggcaatctc
    11221 ctccagcagg gtggtcttac ccgccttcgg aggagagacg atgagtcccc gcgtgccgcg
    11281 tccgatcggc gcgaagaggt cgatgatccg catcgacagg ttcccgagaa ttcccagggg
    11341 aaaccgccgg tccggatcga tcgcggtcaa gctggtgaac gaggaccgct cccggaagcg
    11401 ctcgggaggc aacccgcaga tggtggcgac ggactccagc cgggggccct ggccgccttc
    11461 cacggcggcg ccggtgacgg cggcgcccga gatcagctgg tagcgcgtga tgatgccctg
    11521 ggagacgtag acatcttcgc cgctcggcgc gaacgagcgg gccggatcgc ggagaaagcc
    11581 gccgcctcgt tcgagcacct cgaggtatcc ggacaccggg cgcggcgggc cgctctcgat
    11641 cgggacggcg ggcgcctggt gcgaaccgcg ccggtaagac ggcggctgtc cctgggtcgg
    11701 ccgcggcggt ctcggcccgg gcggccgggg ccccttctgc gacccctgcc gagccctcgg
    11761 gccggatggt cccgccggcc gcggccctgg atgtggccgc tggggcggcc ccttgcccga
    11821 cggtcgggcc ggctgctccc ttccgccgcg ctgctttcct ctacctgatg gcttccgacc
    11881 gccgcctgtc atggaagttc cgttgtaacg catggcggcc gaaaaggaat cctttacttc
    11941 cagggccgga aggcggatac ttcattcgag tggttgcaac ctgacggtcg cacgcagcga
    12001 aagcaaagca cgcgaacctt gccgcaggag aaactgcctg cggctttttc tgtatccgaa
    12061 ccgatgcggt tcgggggcca ggccaaattg gccgggtcgg ccctctcgag agagagggta
    12121 tgttggggcg ttggccccgg tgaacaagga gcacagagtg gctgcaggta cggtgaagtg
    12181 gttcaacgaa tccaagggtt ttggtttcat ccagcaggaa ggcggagagg acgtcttcgt
    12241 tcactactcg gcgatctcgg gcgagggctt caagacgctt gccgaaggcg agcggctgga
    12301 gttcgacgtc gtccgcggtc cgaagggact gcaggcggcg aacgtccgta aggcatagct
    12361 tcacgccccg cgttgcggga ggcggcgccg cgaggcgccg cctccgcgca cgggccactt
    12421 cagcgcttca tcaccccgcg gtatccagct tgagcttccg caccaacgcc ccgtagcggg
    12481 gatcctgccg gattccatcc aggcgcggat ggacccgaag gaagatcagc cccgtggcct
    12541 tttcctcgac cgcgacgtcc agccagcgga acgcctcgtc cacgtcgccc agccgcgcgt
    12601 gcagggccgc gatgccccag gcggacacca cgcgccgcga gcgcgccgcg atcaactcct
    12661 ccagctggcg tcgcgcttcc tccgtgttgc ccatgctgat ctcgaggtgc gcgacgccga
    12721 aatggggtcc tgcgaccccg ccgctcagcc gacggccttc ctcgtattcc cggcgggagt
    12781 cctcgaagcg gcccacggcc tcgagggagc gcgcgaggtc ggtgtgggcg ccgtcgaacc
    12841 ggggatcgat ctccacggcc ttctggtagt agaccagcga tcgctcgtac tcgcgggcgt
    12901 agtagtaggc gtcgccgacg gccgtgtgga tgatcatcga cacgggatcg agacggagcg
    12961 cctgcagcat cgcctcctga gcctcgcggt gacgctccgt gcagtagtag atgctcccca
    13021 gggccatgta cgcgcccggt tccggattca ggtcgatcgc gtgctcgaga ttgcggatcg
    13081 cggcgggaag atcgcgatcc aagatcgcca cttccccgag cgccgagtag gcgtcggcga
    13141 gcgaatcgtc cagctcgagt gccttgagcg cggcggaccg cgcgagcggc gcggcctcgg
    13201 cgggcgcggc catcccccgc tccgcgcgga cgacatggga gtgggcgatc cccgcccacg
    13261 tgggggcgaa ggtgggatcc agcttcaggg cccgatcgta gtagcggagg ctgagctcga
    13321 tggcttgcgg cgaggtggcc gcggcggtgt ggcggccctt caggtactcg acgtgcgcct
    13381 ccggattcac ggccgcctga cgcttggcca gccgcttcgc ctccttcggc gtcacgtgca
    13441 cctggatctc cttcgccacc gcttcggcga cgcggctctg gagatcgaga acgtcctcga
    13501 ggtcgccgtc gtaccggtcc gcccagagcg tctcgtcgct tcgcgcggag atgagctggg
    13561 ccgagacgcg cacccgcttg ccgacgagga gcgccgagcc ctcgagaatc gcgctcacgt
    13621 tcagctcgcg ggcgatctca gggagcgcct tctgggaacc tttgtagcgc atggccgagg
    13681 tgcgcgagat cacccgcagc gccttgagtc gcgcgagctc cgagatcagg gcctcggtca
    13741 tcccgtcggc gaagtattcc tgcgccggat cccgcgagac gttctcgaac gggaggaccg
    13801 ccaacgacgg gagcgcctcg cgaacggact ccccattgcc cccgcgcgat cccggctggc
    13861 cgatgtggcg aagcgtctgg atcacggcca ccgccgacgc ggggcgctgg ccggcgtcct
    13921 tgctcagaca ggcctcgatc aggcggtcca gctcctgcga gatctcttcc cggatcgatc
    13981 gcgccgggag cggcacgcca tgaaggattt cgaacatcag ggcctcgatc cggtccttct
    14041 cgaaagggcg ccgcccggtg gtcatctcgt agagcacggc cccgagcgcg tacaggtcgg
    14101 ttcgcgcgtc gatcacgccg ctggccagct gctccggcgc catgtacggc agcgtgccgg
    14161 ccaccccctg gatgccgctc gaggcggtgg ccagggtctg gccgcctttc tcctggagcc
    14221 gggcgaggcc gaagtcgacc accttggtct gccccttcgg ggtgaccatg atgttgctgg
    14281 gcttgagatc gcgatggatc acgccctgct cgtgcgccgc ttccagcgct tcggcgatct
    14341 ggctccccag gcggacgatc tcggcttcgg ggagcggctc ccgcttcagg tggtccgcca
    14401 gggatttccc caggacgagc tccatcacca ggaaatcgac gccctggtcg gtatcgaagt
    14461 cgtaaacggt ggcgatgtgg gggtggttca agcgggagag cgcgagcgcc tcgcgccgga
    14521 attgccggcg cgcgtcctcg ttggcgagag cgatgttcgg cagaaccttg acggcgacgt
    14581 cccgctccag ccggtcgtcg tgggcgcgat agacctcccc catgcctccg gcgccgagcc
    14641 gttccaggac ctgatagtgc gagagcttcc ggccgatcat cccgtgaatc ataacggacc
    14701 tcggcggaac agtctgtacg cggacgttgt ggcggtccta agctcttggg atgatcagga
    14761 cggccttaac gctgatggcc ggcctctgca ctgccgccgc ctcctggagc ctgccttcag
    14821 gcagtacggg tgcccctctg gcgttcccat cctcggacgc gcccggcgat accgtcacca
    14881 tccacgattg cgtcgccatg gctcgcgagc atgctccggc catccttgcc gccggctccg
    14941 acctcctggc ggcggcctac gaatccacgg cggtggcccg ggagcgcggg ccggattggg
    15001 cgatcacgtc ggacgcgacg gtcgcgccga gaggcttcta cgatcccacg gtcaccgatc
    15061 tcggcggcta tgacctgaag ctggggttcg actggccgct cgccgacggg ggcaagcggt
    15121 tcctcgcgcg ccgtcgcagc gcgctggatc tggcccaggc ccggtggcaa tcgatgcaga
    15181 cggcgagaga cgtcggactg cgcgcgggcg agcttgcggc ggagctcctg cgtcttcagg
    15241 agatcgcggc ggcgcagggc ggcgcggtag agtggacggt gagcctcggc gcgctcgtga
    15301 agcgcggggt gacctcgggg gtgcggagcg ccgccgattc gatccgtgtc gggctggagc
    15361 gggacgccgc ccttctctcc ctggagagca cccttcaaga ggcgcgcacg gatgaattgg
    15421 agctcctcgt tctcctggga atggaccccc tggggcacgt cgtgatcgcg ccggcccctg
    15481 gatccgaccg ccctcccggc gaagacgatt ccctgcgcgt ggtcgcgagc gccgaccggg
    15541 cgcccgaggt cggcctggcc gagatcacgg ccgcgcgcgg cagcctcgat ctcctcgagg
    15601 cgcgccggcg gaacgccgcc acgatcgacc tcgcggccga cgcgggcctg gcgggggccg
    15661 acctgacctc cgccgtgccc gaagatctca aggcggccaa ccctgatgcc accctgggag
    15721 atcgcctccg tcgcgacctc ggagccagcg tggcgttcca cttccggatg ccggtgctgg
    15781 acaagtcggc ggcgcggcac gcccgcgcgc gcgaggaaac gctgcgggcg gcgcgggcca
    15841 aggccctggc ggaacgcggc acgcagcgtc gcgtggccct ggacctgctt tcgcaatgga
    15901 ggttcgccta ccggcgggcg gagctggcgc agcgatcgcg cgaccgcgcc gaggacgccc
    15961 tccttcgcgt gaagagcctc tatgcgggcg gcgcgaccac gctgctcgat ctgctcgacg
    16021 cgcgccgcgt caccgaggag gcgcgggagc gcctggccga agcccggcgg gatgcccgtc
    16081 tcgcgcagct tcgcgtggag gatcgcgaat gaccagatgg cgaatcgcca gggcaggcgg
    16141 cacggtcgtc gcggccctcc tggcagccgc ggcggtcggc ggctgctcga agcacgaaga
    16201 atccgatacc gacgagacga ccgcacgggt tccggtccgg gccgcgcgcg tcgagacccg
    16261 gaccatccag agccagatcg ccgcgacggg gcaatggcgc gcggccgatt ccgtcgtcgt
    16321 gatcgctccc ttcgccgcgt acgtcgagag cgtgcgcccg cacttgggcg atcgcgtcgc
    16381 caagggacag accatcgccg gcctggtcac ccacgagtcg cgcgcggccc tgcgcggcgc
    16441 ggaaatcctc gtgcgtcagg cgtccggggc ggcggagagc caggaggcgc gccgcgccct
    16501 ggcccaggcg gaacgcggga tcgtgcgcgt gcctctgatc gcgtcggtcg cgggaacggt
    16561 gatccggagg agcgccgagc cgggctcgga ggtggcggag agcgcgccgc tcctcaccct
    16621 ggtaccgcag ggctcgttcg tgttcgaggc gcacgtggct catgccgacg cgcgccacgt
    16681 gcggcccggt caggcggcga cgatccggct ggaggcgggc gacgtcatcc ccgccgtaat
    16741 ccagcggctt ctgccgcagg cgagcgagtc cgaccagaac cagctggtat ggctctcccc
    16801 cgggagggtt ccaacggggg cgctcgaccg cttcggcacg gcgagcatcg agaccggctc
    16861 ggtgcgaagc gcggctgccg tgcccgattc ggcgctggtc gaggacgacc tcaccggcca
    16921 ggtgcggatc gcgcgcatcg tgggcgggat cgccatctgg accccggttc gcctggggca
    16981 ggggaacgga ggctggcacg aaatcctggc tccagcgctc ccgcccggca cgccggtcgt
    17041 gatcagcgga cagcggggcc tgccggacag caccaaggtc gagatcgcgc ggtgaccggc
    17101 ttcttcgata cggtccaccg gcagcgccgg gcgctctccc tccttctcgc ctccttgacg
    17161 atcctctcct tcgcggccgt ccggaagctc ccgacgtcca tccttcccga agtcaccttc
    17221 ccgcggatca agatcgtcgc ggacagcggc gagcttccga gcgacgccat gctgcgcagc
    17281 gtcacccgtc cgctggagga atcgctacgc cgcgtgcctg gcgtctccga gatccgctcg
    17341 accaccagcc gcggatccac cgagatcaac ctggacagcc gctggaacgc ggacatgaat
    17401 ctcacgctgc agcgcgtgca ggcccagatg gaagccgtcc ggccggagct gcccgcgggg
    17461 accacgctgg acgcgcgcct gatgaatccg accctctttc cggtactcgg cttttcgctc
    17521 acgtccgatc gcgtctcctc cgcgcagctc cgggacttcg ccaccctgat cctgaagccg
    17581 gagctctcgc ggcttccggg cgtggcggag gtggtgctcc agggcggaca caagctggaa
    17641 gcgcggatca cgctggatcc cgtcgccctc gaagcgcgcg ggctcaccac gcagggcctg
    17701 gtcgaggcca ttcgaaaggc ggcggtgctc gagccggtcg gacttctcga ggccaaccgg
    17761 cagctctacc tgggactcgt cgacagccgc cccaccgacc tcgggaccct cgccacgctc
    17821 cccatcccac tcgcgtccgg agcgcccgtc gccctgggac agctcggcaa gatcacgctc
    17881 gaagaggcgc cggaattcgt ccgctaccgc gcccgccagc gcgaggccgt gctcgtgaac
    17941 gtgctccggc agccctcggc cagcgcgatc gctctgtcgc aagaggcgca ccgctggctg
    18001 accgagcacg cgcgccagct gccgcccggc acccacgtgg agaccttcta cgatcagtcc
    18061 gaccttgtgc gcgcctcggt gggaagcgtg cgcgacagtc tgctggtcgg ggcgattctc
    18121 gcgttcctgg tgatcgtgtt cttcctccga agcgtgcgcc tcgccctcgc aggagccgcc
    18181 gtcctgttct tcagcatcgc cctcaccctg gtcggcctca tgctcgcagg ccagtcgctc
    18241 aacatgatga cactcggggg catcgccgcc gcggtcgggc tggtcctgga tgacggcatc
    18301 gttgtggtcg aacacctcgc cgcgcgcact gcgggaccat cgaccgtgag ccgctcccag
    18361 gccatggcgg agatcttccc ctccctcatc ggatccagcc tctgctcgct cgcgatcttt
    18421 ctcccgttca tctttctggg cggcgtcacc ggcgccttct tccgcgtgct cgcgctctcc
    18481 atgtcgctca tgctcggctt ctcgctcctg ctctgcctga cgctggtacc gctgctgagc
    18541 ccgacgggcc acgccgagcg gccgccccgg acccccgggc ggaccacgga gcgcctacgg
    18601 ctgcttctcg atcggtcgat ccggcgcccg cggatggccg cgctggcggt ggcgctcctg
    18661 atcgttctgg cggttccgct ctacgcgacc ctgggaacgg ggttcctccc cgagatggac
    18721 gagggctcgc tcattctcga ttacgtctct ccccccggca cgtccctgac cgagaccgac
    18781 cggatgctcc aggaagtgga gcggatcatc gcgggcatgc ccgaaatcgc ctcctggtcg
    18841 cgcaggacgg gcgaccagct cggattcttc atcaccgaac cgaacaccgg cgactacgtc
    18901 ctgcggctcc ggtccgggca ccggagaagc gccgacgcga tcgccgacga cctgcgtcag
    18961 cgcatcgaga gcagccagcc ggccctggag gtggagttcg ggcagctcgt ggaggacgtg
    19021 atcggggacc tgacgacgaa cccgcagccc atcgaggtgc gggtcttcag cgaagaccgc
    19081 gcgatcgccg aatcaaaggc gagcgagatc gcgacgctgc tcgaacgggt gcgcggcgtg
    19141 gtcgacgtga aaagcggtgt cgtggtgagc ggtcccacgc tcgtggcgag cccgaatgcc
    19201 acggccgcgc acgccgctct cgacgcgggc gacatggcgg ccgcggccgc tcccgcggtc
    19261 gccggcgtgg acgtgggcga aatcgtccgg ggcgcccggg cgtggagcgt ccgggtggtt
    19321 ctgccgcggc catccgggtt gagcggcgcg gagatcctgg cccgggtccc ggtatccgtc
    19381 gggacgggcg cccacgcgag gctcggcgac ctggccgcgg tcgcgaccaa cccgggcgag
    19441 accgagatcg cgcacgacaa cctgcgcacg atggtggccg tgaccgcgcg cctctccggg
    19501 cgcgacctcg gctcggcgat cgcggagatc caggggcgcg tgcggcgtga actggtgctg
    19561 ccccgcgaag cgtccgtcca gtatgcgggc ctctgggcgg agcagcagtc ttccttccgg
    19621 gggctgaccg gggtggccct ggccgcggcg gccctcgtcg ccctgatcct tctcgcttcc
    19681 tttcaggcct ggcccgagat gatcgcggtg ctcctggtgg tcctggcgtc gctcgcaggc
    19741 gtgttcacgg cgctgcgcct gagcggggcg accttcaaca tctcgagctt tgtcggcgtg
    19801 atcatgatgg tgggcatcgt ttccgagaac gcgtacttcc tggtggcagc gttccaggcg
    19861 cggcttgtgt ccggggattc cccgcccgat gcggcccgcg ccgcggcgct ccgccgggcc
    19921 cgtccggttc tcatgacgac gttcgcagga atcgccgccc tggcgccgct cgccctgggc
    19981 ctgggcgcgg gaagcgccct cttgaagccg ctcgccgtcg cggtggtcgg aggcttctcg
    20041 atctcggcgt tcctgctgct cctggtgctt cccgccctgc tcggtcggtt cgcatcgccc
    20101 tcgcttccgc agccgagagc gcggaatgat ggggccctgc ccacgtaacc cctggaggaa
    20161 ccggcgtgac cgaacgcttc gtgctgtctt cgttctacgg ctacttcgcc gccttcgtgc
    20221 tggtcgagtt cgtgctgacg cggtttcgac tggggacggg agagcgcggg aaccgcgacc
    20281 gcctgtcggc cctgttcttc ctgttcgtgc ccttcgcggg gatattcctg tggatcttct
    20341 gcgtcaacgc ccacctggtc ggcgcacgct cccgatcctc ctgggcgtgc tccagcgcat
    20401 ccctagcgaa gaggcgctcc tggtcgaagg gctgggccag agctatcgcg cctacatgac
    20461 ccggacgaag cggctcatcc ccgggttgtg gtgacgtggg ggcggcctcc ggacggcggc
    20521 ccctccgcta gggcctcttc ccgatcacga gcccggtcgg gccgggcagt ccgaccgtgc
    20581 ggacctccgc gaagcccgcg tcgcgcagcc aggccgcgta ctcctcgccg ctgtaggaat
    20641 ctcccgcgcg cgttcccacc agcatattga gcgcgaacag cgcgcccgtc ttggggcccc
    20701 ccttgtcggc gttcaggatg aagtcatgaa tgacgacgcg gccgccgggg gcgagcgccg
    20761 cgaacgcctt gcccagcatg gcctggttct cttcggaagt gttcctgtgg cagatggcgg
    20821 agaagagcac gaggtcgtag cccgtgccga acgaagggtc gcgcaaatcc ccggtcctga
    20881 cgcgcacctg cgacccgacc cccgcggcct cgatgtagcg ttgcgcgatc ggaaccacat
    20941 ccggcagatc caggacctcg acctccagcc cggggcgcga ccgcgcgaac gcgatggcgt
    21001 aggcgcccga tccgccaccc acgtcgagga cgcgcttcac tccgtccagt ccgatcgccc
    21061 gcgccacgag gggggcgcgg aacgcggcgt tcttgtgcat cgccgcgatg aaggtctcga
    21121 cccagtccgc cccgcgcctg tcgcccaggg tcaccgaagt gccctgacgc acgcactcgg
    21181 tcagcgtgga ccagcgcgac cacaggtgga ccgtgtgcag gagcgcggcc ctcgaatcgt
    21241 cgggcccgcc cgcgaccagg tagcgaccgg cgaccggacc gttgaagaac tccccgtccc
    21301 gtttcgttag gaggtccatc gcggcgagcg cgttcagcag catcccggtc gcccgcggat
    21361 cggtgtcgat cgagcgcgcc acggcggcgg catccgcgcc ggcgccgacc gcggtgaaca
    21421 ggtcgagctc gatggccgtg agcagggtgc ggctctcctg gaacgcgcga agcatctgct
    21481 ggaagtcgtc gggcagcgcg ccggcctgat cccgcgcctt catcccggcc atgcgctttt
    21541 gcccgttctc ct
//
LOCUS       DASSLZ010000101         6552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1429602, whole genome shotgun sequence.
ACCESSION   DASSLZ010000101 DASSLZ010000000
VERSION     DASSLZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429602"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..929)
                     /locus_tag="VFM00_03800"
     CDS             complement(138..929)
                     /locus_tag="VFM00_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497400.1"
                     /translation="MDRDDARSLALPTALLLLGAASAAFDVGCVLIKTPFLRPGVTLG
                     DLIETAGVFVVLALFARVGRLAAGGIASGAALLTGFAAAAFALGHGAHVAANSIHDLA
                     ERSGAGDPTGLLDFWDERVGHYAVDSGRILFAVALLGQPAPSGEGPLAAARGGRASSE
                     SGPTTRRLLPALLVALGGAAYGFTTFASAVEGQTVPLVLPFYALAAVAVVVLARAGRG
                     QPWIRFFFGSAAGTALLFFLIWGIWQHGFPEFTRAGIIHGAGGPG"
     gene            complement(902..1498)
                     /gene="pdxH"
                     /locus_tag="VFM00_03805"
     CDS             complement(902..1498)
                     /gene="pdxH"
                     /locus_tag="VFM00_03805"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET7497401.1"
                     /translation="MSDSTDPIALFQDWFESAHHVGLPEPSAAALATVGADGQPSVRM
                     VLLRGLDERGFVFFTNLNSRKGRELLQPGRTALASLCFYWPPLARQVRVEGRAERVDD
                     AEADAYWATRPRGHQVAAYASPQSEPLPGGRRELEARFAEFDRKLPPKNVARPDYWSG
                     FRVIPDTVEFWEGRENRLHRRVLYMRTDVGWTETTLAP"
     gene            1688..3181
                     /locus_tag="VFM00_03810"
     CDS             1688..3181
                     /locus_tag="VFM00_03810"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HET7497402.1"
                     /translation="MSTRASIKPLSKSRVHAGLQCAKRLYLECYAHEQKDPLDAARQG
                     LIEVGRQVGVAARAAFPGGILIDHDPYHHDDAARATREAMANPAVPAVFEAAFTFDQI
                     RVRVDVIARVDNRSWEVVEVKSSKQVKDEYVTDVAIQLHVVEGSGVPVCGISVLHVNG
                     DYVWPGGPYDPSLLFTRRELIEDARRRLPDLLRRLETMRAGLWGLEPPDIAIGKHCER
                     PHRCPFHGTCHENGVEHPIHTLPRMDQRLRRRLAGLGVADIREIPDDFDGLSMLQRRV
                     RDCLVGGAPFQDPMLREELSKAAYPIHFLDFESCSAPLPVIPGTRPFEPTPFQWSDHV
                     LHENGDVEHREYLHDARTDPRRPLTTALLEALDGAGAIVVYSGFEARTMRELAATLPD
                     LAPPLLAHVEHRIVDLHRLLHLHYYHPDLRGSFSIKDVLPVVVDGLGYGDLVIKDGSQ
                     ASLAFSDMTDPGLPAEARDLLRERLLAYCGRDTEATMRLFLALKEGA"
     gene            3178..3828
                     /locus_tag="VFM00_03815"
     CDS             3178..3828
                     /locus_tag="VFM00_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497403.1"
                     /translation="MSGRRMFMALCVTAVAAGPVRADVRPPLPGRTYTWTFEADTLGQ
                     APASTRATGGRWAVLEDSTQAGARLLRQMDFEEGLGSHALQFLKPRVADQQTSVRYRI
                     RSGGMDPSVGLAFHLDAKGKNGYLVRVSGRSQELIAHYLLNGKRRDLKMVPIEPPRPD
                     EWHTLGVRRVGERLEVLYDGVVKMKLRDERFGAGNVGLWTEDDTVADFEGFTVSTL"
     gene            complement(4136..5530)
                     /locus_tag="VFM00_03820"
     CDS             complement(4136..5530)
                     /locus_tag="VFM00_03820"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET7497404.1"
                     /translation="MKLDSVFRYLDENKDRLTDELIQFLRIPSISAHPDRDADVAAAL
                     DYDKRKLESLGFRTEVWPTRAHAGLFAERIENPDLPTVLIYGHVDVQPVDPVELWESP
                     PFEPRIAGGKIWARGADDNKGQHYAQIAGVEAAIRAGGGLPVNVKFIIESDEEYDGEA
                     MAAEFPKHGKALACDAIVVSDSSMPDLDHPALTTYLRGIQAFEITITGATGDKHSGEW
                     GGMLHEPVDVLRYVLQSLKDFKTGKVLIPGFYDDVVVPSAEERQAMSNAPWDDAARAR
                     EIGVARLFHEEGYTPRESGVIRPTLQTNGIWGGYTGPGFKTVIGNVAKAKISMRLVHN
                     QDPEKIAQLFEKRVRELVGDMGTVTFQRFGAGLPFMSDLQNPFVQGGLKALEAGFGRP
                     AVVMGMGGSIPIVAPLVKATGAPCVLMGFGLPGDNLHAPNEHFALSCFFGGAKSAAAY
                     LYEVASRAGISSSR"
     gene            5911..6084
                     /locus_tag="VFM00_03825"
     CDS             5911..6084
                     /locus_tag="VFM00_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET7497405.1"
                     /translation="MLKNFIRDERGEDLIEYGLLAAFVAAIATAVIIADPLGLRTAVE
                     GAYTAAVTALNAM"
     gene            6118..>6552
                     /locus_tag="VFM00_03830"
     CDS             6118..>6552
                     /locus_tag="VFM00_03830"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET7497406.1"
                     /translation="MAGGAVILCGVASWTDLRSRRIPNALTIPALVAALCVHGALGAG
                     QGLLLSACGALAAGALLIPGYLFRFTGAGDVKLLMAVGAILSFPTALLAGLASLIFGG
                     LLGAVTAFRLGRLREVLGRTAGLARWAAVRATGVPVAAPETSR"
BASE COUNT          985 a         2262 c         2265 g         1040 t
ORIGIN      
        1 gggggcggcc ttcgcggccg gcgcgctctt cgaggccggc gcgtccttgg tcgctccctt
       61 gggggcaggc gcgccccagg tcgcactaac tggcgccagc gtggcgcaga tcacgagcgc
      121 cgcgaggaag gtgcccttca tcccggtcct cccgcgccgt gaatgatccc cgcgcgcgtg
      181 aactcgggga agccgtgctg ccagatcccc cagatcagga agaagaggag cgccgtgccg
      241 gcggccgagc caaagaagaa tcggatccag ggctggccgc gcccggcgcg cgcgaggacg
      301 acgaccgcca ccgcggccaa tgcgtaaaac ggaagcacca gcggcaccgt ctgcccctcg
      361 accgccgagg cgaacgtcgt gaatccgtac gccgcgccgc ccagggcgac gagcagcgcg
      421 ggtaggagcc ggcgcgtcgt aggtccggac tcgctcgaag cgcgtcctcc tcttgcggcc
      481 gcgagcggtc cctccccgct gggggcaggt tgccccagca gagcgaccgc aaagagaatc
      541 cgtccgctgt ccacggcgta atgcccgacg cgctcatccc agaagtcgag cagaccggtg
      601 gggtcgcccg cgccgctccg ctccgcgaga tcgtggatcg agttcgcggc cacgtgcgct
      661 ccatgtccca gggcgaaggc ggcggcggcg aaccccgtga ggagtgccgc gccgctggcg
      721 atgccgcccg ccgccagccg cccgacccgc gcgaaaagtg ccagcaccac gaacacgccg
      781 gccgtctcga tcaggtcgcc cagcgtgacg cccggacgca ggaagggagt cttgatcagc
      841 acgcagccga cgtcgaaggc ggccgacgcc gcgcccagga ggagcaaagc ggtggggagc
      901 gctagggagc gagcgtcgtc tcggtccacc cgacgtccgt tcgcatgtag aggacgcggc
      961 gatgcagccg gttctcgcgg ccctcccaga actcgaccgt gtccgggatc acgcggaatc
     1021 ccgaccagta gtcggggcgg gccacgttct tcggcggcag cttccgatcg aactcggcga
     1081 agcgggcctc cagctcccgc cgtcctccgg gaagaggctc gctctggggc gaggcgtacg
     1141 cggccacctg gtgcccgcgc ggccgcgtcg cccagtacgc gtcggcctcg gcgtcgtcca
     1201 cgcgctcggc gcgcccttcg acgcgcacct ggcgcgcgag cggcggccag tagaagcaga
     1261 gcgacgcgag cgccgtccgt cccggctgga gcagctcccg gcctttccgg ctgttcaggt
     1321 tggtgaagaa gacgaagccc cgctcgtcga ggccgcggag gagcaccatg cggacggagg
     1381 gctgcccgtc ggcgcccacg gtggcgagcg ccgcggcgga cggctccggc agtcccacgt
     1441 ggtgggcgga ctcgaaccag tcctggaaca gcgcgatggg gtcggtggaa tcgctcacgg
     1501 gcggagcgat cgtacaccag cccccgcgga cgcgatatac tcgggtcggt cggcgcggcg
     1561 ggctcactga catcacgctg cagttcgccc gggtccgccc gcgcccggcc acatccccct
     1621 gtgctatcgt tttcggggtc atgtacccgt ttaagccatg agccaagccc tgcttcggga
     1681 aggtgcattg tccacccgcg cctcgatcaa gccgctctcc aagtcccggg tccacgcggg
     1741 gctgcagtgc gccaagcgcc tctatctcga gtgctacgct cacgagcaga aggacccgct
     1801 cgacgccgcc cgccaggggc tgatcgaggt cgggcggcag gtcggggtgg cggctcgggc
     1861 ggcgttcccc ggcgggatcc tcatcgacca tgacccctac caccacgacg acgccgcccg
     1921 ggccacgcgc gaggcgatgg cgaaccccgc cgtgcccgcc gtcttcgagg cggccttcac
     1981 gttcgaccag atccgggtcc gcgtggacgt gatcgcgcgc gtggacaacc ggagctggga
     2041 agtggtcgag gtcaagtcga gcaagcaggt gaaggacgag tacgtgaccg acgtggcgat
     2101 ccagctccac gtcgtcgagg gctcgggcgt cccggtatgc gggatttccg tcctccacgt
     2161 gaacggggac tacgtgtggc ccggggggcc ctacgatccc tcgctccttt tcacgcggcg
     2221 cgagctgatc gaggacgccc gacgccgcct gcccgacctg ctgcggcggc tcgagaccat
     2281 gcgcgcgggc ctctggggtc tcgagcctcc ggacatcgcg atcgggaagc actgcgagcg
     2341 gccgcatcgc tgccccttcc acggcacctg ccacgagaac ggcgtcgagc acccgatcca
     2401 caccctgccg cggatggacc agcggctgcg gcgccggctc gccgggctcg gcgtcgccga
     2461 cattcgcgag atccccgacg acttcgacgg actgagcatg ctccagcgcc gcgtgcgcga
     2521 ctgcctggtc ggcggagcgc cgttccagga cccgatgctc cgggaggagc tgtcgaaggc
     2581 cgcctatccg attcacttcc tcgacttcga atcgtgcagc gcgccccttc cggtcattcc
     2641 cgggacgcgc cccttcgagc cgacgccgtt ccagtggagc gatcacgtgc tccacgagaa
     2701 cggcgacgtg gagcatcgcg aatatctgca cgacgcgcgc accgatccgc gccggccgct
     2761 caccacggca ctcctcgaag cgctcgacgg cgcgggcgcc atcgtggtct attccggatt
     2821 cgaggcgcgg acgatgcggg agttggccgc gacgctgccc gatctcgcgc cgccgctcct
     2881 ggcgcacgtc gagcatcgga tcgtcgacct gcaccggctc ctgcacctcc actactacca
     2941 tcccgacttg cgcggctcgt tctcgatcaa ggacgtcctc cccgtcgtcg tggatggcct
     3001 ggggtacggc gacctggtga tcaaggacgg gagccaggca tcgctggcgt tctcggacat
     3061 gaccgatccc ggcctgccgg cggaagcgcg cgaccttctg cgcgagcgcc tgctggccta
     3121 ctgcggacgc gacacggagg cgacgatgcg gctgttcctg gctttgaagg agggcgcgtg
     3181 agcgggcgga ggatgttcat ggcgttgtgc gtcacggccg tggccgcggg ccctgttcgg
     3241 gccgatgtcc gccctccgct cccggggagg acctacacct ggacgttcga ggccgacacc
     3301 ctgggccagg cgccggcctc cacgcgcgcg accggcggac ggtgggccgt gctggaggac
     3361 tcgacgcagg ccggcgcgcg cctgctgcgc cagatggatt tcgaggaagg gctgggatcg
     3421 cacgcgctcc agttcctgaa gccgcgcgtc gccgaccagc agacgtccgt gcgctaccgg
     3481 atccgctcgg ggggaatgga tccctcggta gggctcgcct tccatctcga cgcgaaaggg
     3541 aagaacggat atctcgtccg cgtcagcggc cggagccagg agctgatcgc gcactatctc
     3601 ctgaacggca agcggcgcga tctgaagatg gttccgatcg agccgccgcg ccccgacgag
     3661 tggcacacgc tgggcgtgcg gcgcgtgggc gagcggctcg aggtgctcta cgacggcgtc
     3721 gtgaagatga agctgcgcga cgagcgattc ggggcgggga acgtgggact ctggacggaa
     3781 gacgacacgg tggccgattt cgaggggttt accgtcagca cgctgtagcg gccggtggcg
     3841 agcgtgccgg gcggcgggga ccgccggagc tcacaccgta cgccccaggc gcaaattagg
     3901 ggcgcgccgt tacgggaggg tggagtcggg tgccggtgag aaccacgagc tcgggaccct
     3961 gccgccgttc gcgccccgtc ttccgtgact ggggcacatc ctccccggcc gtccctggcc
     4021 ggaggggcgc acctcaatag gctttcccta attggatttc ctaggtctcc taccgtttag
     4081 atgccgaccc ccaaggcgca ggatacttaa tttcaaaagc acaacaagtg tatttctaac
     4141 ggcttgacga tattcctgcc cgggaggcca cttcgtagag gtaggccgcc gccgacttgg
     4201 cccccccgaa gaagcaggaa agcgcgaagt gctcgttggg ggcgtggagg ttgtctcccg
     4261 gcaacccgaa gcccatcagc acgcaagggg cgcccgtcgc cttgaccagc ggcgccacga
     4321 tcgggatcga tccgcccatt cccatgacga ccgccggcct gccgaaaccc gcctccagcg
     4381 ctttcaaccc gccttggacg aagggattct ggaggtcgga catgaagggc agcccggcgc
     4441 cgaagcgctg gaaggtcacc gtgcccatgt cgccgaccag ctcgcgcacg cgcttctcga
     4501 agagctgggc gatcttctcc gggtcctggt tgtggacgag gcgcatgctg atcttggcct
     4561 tggccacgtt cccgatcacc gtcttgaacc ccggccccgt gtagccgccc cagattccgt
     4621 tggtctgcag cgtggggcgg atcacgcccg actcgcgcgg cgtgtagccc tcttcatgga
     4681 agagtcgcgc cacgccgatt tcccgcgcgc gcgccgcgtc gtcccagggc gcgttgctca
     4741 tcgcctgccg ctcctcggcg ctcggaacga ccacgtcgtc gtagaacccg gggatgagca
     4801 ccttgcccgt cttgaaatcc ttgagcgact ggagcacgta gcggagaacg tccaccggct
     4861 cgtggagcat cccgccccac tcgcccgagt gcttgtcgcc ggtggccccg gtgatcgtga
     4921 tctcgaaggc ctggatgccg cgcagatagg tggtcagcgc gggatgatcc aggtcgggca
     4981 tgctcgagtc ggacaccacg atggcgtcgc aggcgagcgc cttcccgtgc ttggggaact
     5041 ccgcggccat cgcctcgccg tcgtattcct cgtcgctctc gatgatgaac ttcacgttca
     5101 cgggcagccc gccgcccgct cgaatcgccg cctcgacgcc cgcgatctgc gcgtagtgct
     5161 gtcccttgtt gtcgtcggcg ccgcgcgccc agatcttccc gcccgcgatc cgcggttcga
     5221 agggcggcga ctcccagagc tcgaccggat ccaccggctg cacgtcgacg tggccgtaga
     5281 tcagcaccgt cgggaggtcg gggttctcga tccgctcggc gaagagcccc gcgtgcgcgc
     5341 gcgtcggcca cacctcggtg cggaatccga gcgattccag cttccgcttg tcgtagtcga
     5401 gcgcggccgc gacatcggcg tcccggtcgg gatgagcgct gatgctggga atacggagga
     5461 attggatcag ctcgtcggtg agtcggtcct tgttctcgtc gagatagcgg aacaccgagt
     5521 cgagtttcat gcgcgctcct gggtcggtgg aatcgcggaa gcggttggat acgaccatcc
     5581 tagcggcaca acgacttggg gacaattccc gccttcgatt ggggcatctc ccccagtttt
     5641 cggctttccg gcgtgcgtcc cgcgcggccg atgcgctttt cgctcctgct aattcctgtg
     5701 cccgccgata ggtagggacg cgtagcgctc cgcgacgaac tgcggcaccg aagttgccct
     5761 tgcatggatg cgcgcgtggt ccaaacgcct cgcggcggag gtcatccgcc acgcgccggt
     5821 agtgggccgc tcgcccggaa gagaccgaac ttgaaacggg ccgggtcggg caaacgcagt
     5881 accacagtct tgtagtggga gggtgcttca atgctgaaga acttcattcg ggacgagcgt
     5941 ggcgaagatc tcatcgagta tggccttctg gcggctttcg tcgcggcgat cgcgacggcg
     6001 gtcatcatcg cggatcccct cggcctccgt acggcggtcg aaggtgccta cacggccgcg
     6061 gtgaccgcgc tcaacgcgat gtaattcgaa gtcaccatcg acatgacctc acattggatg
     6121 gccggcggcg cggtgatcct gtgcggtgtg gcgagttgga cggacctgcg ttcgcgcaga
     6181 attccgaatg ctctgaccat cccggccctc gtagcggcgc tgtgcgtgca cggcgccctc
     6241 ggggccgggc agggccttct cctctcggca tgcggcgcac tggcggcggg agcccttctg
     6301 atcccgggct acctgttccg cttcacgggc gccggagacg tgaagctcct gatggcggtc
     6361 ggcgcgattc tctcgtttcc gacggcgctg ctcgcggggc tggcctcgct gatcttcggc
     6421 gggctgctcg gcgcggtgac cgccttccgc ctgggaaggc ttcgcgaagt gctgggtcgc
     6481 acggcggggc tcgcccgctg ggcggccgtc cgggcgaccg gggtgccggt ggccgctccg
     6541 gagacttcca gg
//
LOCUS       DASSLZ010000102         5026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1557213, whole genome shotgun sequence.
ACCESSION   DASSLZ010000102 DASSLZ010000000
VERSION     DASSLZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557213"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFM00_03835"
     CDS             complement(<1..737)
                     /locus_tag="VFM00_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET7497407.1"
                     /translation="MTQLAPAYPFLRACRNEPSSETPIWIMRQAGRYLPEYRALREKH
                     GFLEFCTVPELCAEVTAQPIDRFGFDAAILFSDILIPVIGLGVPIAFAPGPQLEFRIE
                     RREDVARLHWKGAAESTPHVKPVIRAVRERLAGRVPLIGFGGAPFTIASYLVEAGGSK
                     ELTKTRGFLSRDPEWFARLLDIIVDATIDYLKAQIAAGVDAIMLFDTQAGALPPAEFA
                     RTAAASAARVLAALPKNMPTIYFALAPA"
     gene            complement(734..2044)
                     /gene="hemL"
                     /locus_tag="VFM00_03840"
     CDS             complement(734..2044)
                     /gene="hemL"
                     /locus_tag="VFM00_03840"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET7497408.1"
                     /translation="MTEARIVPKTRESEILGHRAAGLFPGGVNSPVRAFKGVGGTPRF
                     IASAKGAWVTDEDGNRLLDYVLGWGPLLLGHAHPAVVDAVKRQAPLGTLYGASTKLEI
                     ELAERVRRFVPAAERIRFVSTGTEATMSALRVARAATGRSAYVKLDGCYHGHADSFLI
                     QAGSGLATLGTPDSPGVPETTAEEARIASFNDLDSVAAHFTREPEGIAAVIVEPVIGN
                     YGVLAPEPGFLPGLRALCDRYGAVLIFDEVMTGFRASRGGAAERYAVRPDLVTLGKVI
                     GGGLPLAAFAGRADLMERVAPQGPVYQAGTYSGNPLSMAAGNAALEILEKEPEIFRRV
                     EARTTLLAAGLREILARRRIPGAVNAVGSMWTLFFGPARVTSMKDVRAADKQRFARFF
                     HGMLERGVSLPPSAFESAFLSDAHTDEDIDRTLEAADEAMEGLS"
     gene            complement(2041..3024)
                     /gene="hemB"
                     /locus_tag="VFM00_03845"
     CDS             complement(2041..3024)
                     /gene="hemB"
                     /locus_tag="VFM00_03845"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET7497409.1"
                     /translation="MTFPTTRLRRLRATPALRAMTRETTLDAADLVAPLFVAHGDTAR
                     REIASMPGHAQLSVKEAVREAKTLADLGVGGVILFGIPKAKDAEGTEAYAEDGIVPRA
                     LRAIKEAAPELLLWADVCLCEYTDHGHCGVIRDGRVDNDRTLPLLARASVAYARAGAD
                     LIAPSDMMDGRVGSIRRALDEAGLDETPIVAYSAKYASAFYGPFREAAESAPQFGDRR
                     GYQMDPANGDEAMREVALDVEEGADMVMVKPALPYLDVIRRVKERFGMPVAAYNVSGE
                     YSMIKAAAERGWLDGERAALEVLTSIRRAGADVILTYFAKEVAPLLGRRAR"
     gene            complement(3021..3806)
                     /locus_tag="VFM00_03850"
     CDS             complement(3021..3806)
                     /locus_tag="VFM00_03850"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET7497410.1"
                     /translation="MSEARPFLIVARSEPSDDDLIRAAEGQGFDVARLELFETRPGGQ
                     VDALAERLKKVEPGTAVAWTSRRAAVVLAQTALPARREALQAIPLYAVGEESAAPIRG
                     AGLEVSVPAGGLGAGHLAAYLVEHAQALGVKRVLFLHGDRALTDFPDALARAGISVEK
                     YELYRTVYLPADPAPVVRALDARRPCVIAYYSPSGIAGLERLLDPAMGEVLHSEAIAI
                     ARGETTYQALLRWGYTHAVRPTGARQHDAFVLDTIQSVARSLH"
     gene            complement(3803..4726)
                     /gene="hemC"
                     /locus_tag="VFM00_03855"
     CDS             complement(3803..4726)
                     /gene="hemC"
                     /locus_tag="VFM00_03855"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET7497411.1"
                     /translation="MRIGTRGSALALAQSGHVRERLTNGAPDDIPLEVIETSGDRNPM
                     ASLRRFGGKGIFTKEIEEALLLGRVDLAVHSLKDLPTEMTAGLMIGAMLPREDPRDAV
                     IARGGTPLEKLPAGATVGTSSLRRKAQLLAKRPDLNVVDLRGNVPTRLARLEEGRFDA
                     IVVAAAGLRRLNLIDRATELLDDAFMLPAPGQGVVAIQIREGDPETAAAVGRLHDPGA
                     AAEAAAERSLLSALGGGCLVPIGARAKIEDGTVTLAGFVGDPSGRPSLRRTAHGPASD
                     PEAVGRELAGMFLHEGARAILDKARGDEVFP"
     gene            complement(4704..>5026)
                     /locus_tag="VFM00_03860"
     CDS             complement(4704..>5026)
                     /locus_tag="VFM00_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497412.1"
                     /translation="IVEEELSRFLRWHRSLAVVPTVTAFRTQVEAIAHQELERHKGKF
                     RAEDHAALDALVHGIVQKVLHRPTTKLTRADEKAPDGIARIDAIRDLFGIEEEDSDAD
                     RNPR"
BASE COUNT          725 a         1809 c         1778 g          714 t
ORIGIN      
        1 gcgggggcga gggcgaagta gatcgtcggc atgttcttgg gaagggccgc gagcacgcgc
       61 gccgccgaag ccgccgcggt gcgtgcgaac tccgccggcg ggagcgcccc cgcctgggtg
      121 tcgaacagca tgatggcgtc cacccccgcg gcgatctgcg ccttcaaata gtcgatcgtg
      181 gcgtccacga tgatgtcgag gagccgcgcg aaccactcgg gatcgcgcga gaggaatccg
      241 cgcgtcttgg tcagctcctt cgagccgccg gcctcgacca ggtagctcgc gatcgtgaag
      301 ggcgccccgc cgaacccgat cagcggcacc cgtccggcga gccgctcccg tacggcgcgg
      361 atcaccggct tcacgtgggg cgtggactcg gccgcgccct tccaatggag ccgcgccacg
      421 tcctcgcgcc gctcgatccg gaattccagc tgcggccccg gagcgaacgc gatcggcacg
      481 cccagcccga tcaccggaat caggatgtcc gagaagagga tcgccgcgtc gaagccgaag
      541 cggtcgatcg gctgcgccgt gacctcggcg cacagctcgg gcaccgtgca gaactccaga
      601 aagccgtgct tctcgcgaag cgcccggtac tcggggagat agcgtccggc ctggcgcatg
      661 atccagatcg gcgtctccga gctgggctcg ttgcggcacg cgcggagaaa cgggtaagcg
      721 ggcgcgagct gggtcatgag agcccctcca tcgcctcgtc ggcggcttcc agcgtcctgt
      781 cgatgtcctc gtcggtgtgc gcgtcggaga ggaacgcgct ctcgaacgcg gagggcggaa
      841 gagagacgcc ccgctccagc atgccgtgga agaagcgggc gaagcgctgc ttgtcggccg
      901 cgcgaacgtc cttcatcgag gtcacgcgcg ccggcccgaa gaaaagggtc cacatggagc
      961 ccacggcatt caccgcgccc gggatccggc ggcgcgcgag gatctcgcgg agccccgcgg
     1021 ccaggagcgt cgtgcgcgcc tcgacgcgcc ggaagatctc cggctccttc tcgaggatct
     1081 ccagcgccgc gtttcccgcg gccatggaga gcgggtttcc ggagtaggtg ccggcctggt
     1141 agacgggccc ctgcggcgcc acgcgctcca tgagatcggc gcgccccgcg aaggccgcga
     1201 gcggaaggcc gccgccgatc accttgccca gcgtgaccag gtcgggacgc acggcgtagc
     1261 gctcggccgc gcccccgcgc gaggcgcgga agccggtcat gacctcgtcg aagatgagca
     1321 cggcgccgta ccggtcgcag agcgcgcgga gcccggggag gaatcccggc tcgggcgcca
     1381 gcacgccgta gttgccgata accggctcca cgatgacggc ggcgatcccc tcaggctcgc
     1441 gcgtgaagtg cgcggccacg gagtcgagat cgttgaacga ggcgatgcgc gcctcctcgg
     1501 ccgtggtctc cggaacgccg ggcgaatcgg gggtgcccag cgtcgcgagt cccgaacccg
     1561 cctggatcag aaaggaatcg gcgtggccgt ggtagcagcc gtccagcttc acgtacgcgc
     1621 tccggcccgt ggcggcgcgc gcgacgcgga gcgcgctcat cgtggcctcg gtgcctgtcg
     1681 agacgaaccg gatccgctcg gcggcgggga cgaagcggcg gacgcgctcg gccagctcga
     1741 tctcgagctt cgtgctcgct ccgtacaacg tgccgagcgg agcctggcgc ttcaccgcgt
     1801 ccaccaccgc ggggtgcgca tgccccagca ggagcggccc ccatcccagg acgtagtcga
     1861 ggagccggtt tccgtcctcg tccgtcaccc aggcgccctt ggcggaggcg atgaagcggg
     1921 gcgttccccc cacgcccttg aaggcgcgca cgggagagtt cacgccgccc gggaagaggc
     1981 cggcggcgcg gtggccgagg atctcgctct cgcgcgtctt gggaacgatg cgcgcttccg
     2041 tcatcgcgct ctccgtccca ggagcggagc gacttccttg gcgaagtagg tcaggatcac
     2101 gtcggcgccg gcgcggcgga tcgaggtgag cacctcgagc gcggcgcgct cgccgtccag
     2161 ccagccccgc tcggcggcgg ccttgatcat cgagtactcc ccggagacgt tgtaggcggc
     2221 gaccggcatg ccgaagcgct ccttcacgcg gcggatcacg tcgaggtagg ggagcgccgg
     2281 cttcaccatc accatgtcgg ccccttcctc gacgtccagc gccacctcgc gcatcgcctc
     2341 gtccccgttc gccgggtcca tctggtagcc gcgccggtcg ccgaactgcg gcgccgactc
     2401 ggcggcctcg cggaaggggc catagaaggc gctcgcgtac ttggcggagt aggcgacgat
     2461 cggcgtctcg tccagccccg cctcgtccag cgctcggcgg atggagccca cccgcccgtc
     2521 catcatgtcg cttggcgcga tgaggtcggc gccggcgcgc gcgtaggcga ccgaggcgcg
     2581 cgccaggagc gggagcgtgc ggtcgttgtc cacgcgtccg tcccggatca cgccgcagtg
     2641 cccgtggtcg gtgtactcgc agaggcagac gtccgcccac aggagcagct cgggggccgc
     2701 ctccttgatg gcgcggagtg cgcgcgggac gatgccgtcc tcggcgtacg cttcggtccc
     2761 ctcagcgtcc ttggccttcg ggatgccgaa gaggatcacg ccgcccacgc ccaggtccgc
     2821 gagcgtcttc gcctcgcgca ccgcttcctt caccgagagc tgggcgtgtc caggcatcga
     2881 ggcgatctcg cggcgcgccg tgtcgccgtg cgcgacgaag agcggcgcca ccaagtcggc
     2941 ggcgtcgagc gtggtctcgc gcgtcatggc gcgcagcgcg ggggtcgccc gcaggcggcg
     3001 gagtcgggtg gtcgggaagg tcaatgaagg ctcctcgcta ccgattgtat cgtgtcgaga
     3061 acgaacgcgt catgctgccg ggcgccggta gggcgcacgg catgcgtgta cccccagcgc
     3121 agaagggcct ggtaggtcgt ttcgccccgc gcgatcgcga tcgcttccga atgaaggacc
     3181 tcacccatcg cggggtcgag cagccgctcc aggcccgcga ttcccgaggg actgtagtag
     3241 gcgatgacgc agggacgccg cgcgtccagc gcccgcacca ccggcgcggg gtcggccggc
     3301 aggtacaccg tgcgatagag ctcatatttc tcgacggaga taccggcgcg cgccagcgcg
     3361 tccggaaagt cggtcagcgc gcgatcgcca tgcaggaaga ggacgcgctt tacgccgaga
     3421 gcctgcgcgt gctcgaccag gtaggcggcc agatgtcccg cgcccagccc tccggcgggc
     3481 accgaaacct ccagtcccgc gccgcggatc ggcgccgcgc tctcctctcc gaccgcgtag
     3541 agcgggatcg cctggagcgc ttcgcgccgc gccgggagcg ccgtctgggc cagcaccacg
     3601 gccgcgcggc ggctggtcca cgcgacggcg gtgcccggct cgaccttctt caaacgctcc
     3661 gcgagcgcgt ccacctgccc gccgggacgc gtctcgaaca gctcgagccg cgccacgtcg
     3721 aatccctgac cttcggccgc gcggatcagg tcgtcgtcgg acggctcgct ccgtgccacg
     3781 atcaggaagg gacgcgcctc gctcacggaa agacctcgtc tccacgggcc ttgtccagga
     3841 tggcgcgtgc gccttcgtgg aggaacatcc ccgccagctc gcgccccacc gcctccggat
     3901 cggaggcggg accgtgcgcc gtgcgccgca gcgacggccg tcccgaagga tcgccgacga
     3961 aacccgcgag cgtgaccgtg ccgtcctcga tcttcgcccg cgcgccgatc gggaccaggc
     4021 agccgccgcc cagcgcggag aggagcgacc gctcggccgc ggcctcggcg gcggcgcccg
     4081 gatcgtgcag tcggcccacg gccgccgcgg tttccggatc cccttcgcga atctggatcg
     4141 ccaccacgcc ctgcccgggc gcggggagca tgaaggcgtc gtccaggagc tcggtcgcgc
     4201 gatcgatcag attcagccgc cgaagtcccg ccgccgccac cacgatcgcg tcgaaacgcc
     4261 cctcttccag cctggccagg cgggtcggca cgttcccgcg caggtccacg acgttcaggt
     4321 cggggcgctt cgccagcagc tgcgccttgc gccggaggga tgaggtgccc accgtggcgc
     4381 ccgcgggcag cttctcgagc ggcgttcctc cgcgcgcgat caccgcgtcc cgcggatctt
     4441 cgcggggaag catcgcgccg atcatgagcc cggccgtcat ctcggtcggc aggtccttga
     4501 gcgaatggac cgcgaggtcc acgcgcccca gcagcagcgc ctcctcgatc tccttggtga
     4561 agatcccctt gccgccgaac cggcggagcg aggccatcgg attgcgatct ccggaggtct
     4621 cgatcacctc gagcgggatg tcgtcgggag cgccgttcgt caggcgctcg cgcacgtggc
     4681 cgctctgggc cagggcaagc gcactaccgc gggttccgat ccgcatcgga atcctcctcc
     4741 tcgattccga acagatcgcg gatggcgtcg atccgggcga tcccgtcggg ggccttctcg
     4801 tcggccctcg tgagcttggt cgtgggacga tggagcacct tctgcacgat gccgtggacc
     4861 aaggcgtcca gcgcggcgtg gtcttcggca cggaacttcc ctttgtggcg ttccagctcc
     4921 tgatgcgcga tcgcttccac ctgggtccgg aacgcggtga cggtgggcac gaccgccagc
     4981 gagcggtgcc agcgcaggaa gcgcgacagc tcctcctcga cgatcg
//
LOCUS       DASSLZ010000103         4382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_36189, whole genome shotgun sequence.
ACCESSION   DASSLZ010000103 DASSLZ010000000
VERSION     DASSLZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36189"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..361)
                     /locus_tag="VFM00_03865"
     CDS             complement(17..361)
                     /locus_tag="VFM00_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014502245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HET7497413.1"
                     /translation="MSDCLFCRIVSGEIPSKKVADSADLLAFRDIAPQAPTHILIIPK
                     RHIAASAAELTPEQGALLGELFPFAARVASGEGLGSGWRLVTNVGPHAGQTVFHLHFH
                     LVGGAPLGRFGT"
     gene            complement(471..2327)
                     /locus_tag="VFM00_03870"
     CDS             complement(471..2327)
                     /locus_tag="VFM00_03870"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HET7497414.1"
                     /translation="MIRRTAIWMTMAAAVVLAGCSSRRGDQLSQDDYPYAGPPSDTYG
                     DAGGASSGRAASSGTYASRTGRDYRRGGTDYYGRWAYSYGRDPSYDYYLASSYGGYDD
                     DYDDYGDDEYGYPVTVSYFYDELDPYGDWYDDPRYGWVWYPEAVSAGWRPYLNGYWVY
                     TDWGWAWVSDDPWGADPYHYGRWACDASNRWMWVPDDVWAPAWVSWRYGGGYAGWAPL
                     PPEATWDVSFGIRYADDFVAPSRWCFVPESRLTSTRLRTYVAPLRDTRRIYTETRDLT
                     RYASVSGRVAERGLRPALIERATGRDIPQYRLTTRESGLRRDMVRGRTLVSPMPVLSR
                     GGGRGRAGGERLTSEMRTRRDRFEREMRVRGGRLERGQRVPPGRGGETQARGRIERGA
                     PVDRQGDFERPARMGQGRQDQMREQRARNAREERAIRQQQRFDRDQRQRGYERQQQRV
                     DEREQRQRGYERQQQQQRYDRQQQDQQRYDRQQQLDRGRSEQRRRDRAEAEGRARADQ
                     QAQRDQARQQAEQARYERQQADQARYERQQARQQAEQDRYQRQQQQQDARARQEERRQ
                     QQVQQQQQEQPQQQQERRRGRQDRGNSDQGSAPSRDQGSDQGGHGRGRGHNH"
     gene            2529..3782
                     /locus_tag="VFM00_03875"
     CDS             2529..3782
                     /locus_tag="VFM00_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7497415.1"
                     /translation="MGKKIVIVGGGVSGLATAWYCLERGHDVTILEQGPEHHDCCSLG
                     NAGFISPSHFIPLASPGIVGKALRWLGDPESPFWVRPRLDPSFLAWSYRFWRASRPER
                     ARRAGPLLRDLNLASRSLFEELARRTGNDFGLTEEGLLLLFRSEAALDVERRHAAAAR
                     ELGLEAVVLDRAGVAALEPGLTLDVTGGVHYPMDAHVTPQRFCATLARIVRERGGDFA
                     WESGVTGWRASGRRVEAAITPRGEHRGDEFVVAAGSSSRSVLRPLGVPIALEPGKGYS
                     LTLPIPPERPRRSLLLQEARVAITPMGSTVRVGGTMELGAGTLGVNPPRIRGIVRSMG
                     RYLPAFTPDRFAGIEPWSGLRPCTPDGLPYIGRSARLDNLLVATGHAMLGLSLGPVTG
                     SLIAALVSNERPAIDIAPLRPERFG"
     gene            3779..>4382
                     /locus_tag="VFM00_03880"
     CDS             3779..>4382
                     /locus_tag="VFM00_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7497416.1"
                     /translation="MNRFGKAIRSEWLLDPAYVYLNHGTVGAPPRRAFEAQERIRREV
                     ERQPARFLLRELSAIAVGGPRAEPPRMRAAAERVARFLNAEGKDLVFVDNATTAINAV
                     LRSFPFRAGDEIVISDHAYGAIRNAAAFRARETGAVVRDVALPGPPFEAARIAEAYAA
                     ALSPRARLLIVDHVTSESALILPIETIVAEAKARGVRVLVD"
BASE COUNT          565 a         1588 c         1536 g          693 t
ORIGIN      
        1 acgaatcggc ggcgtctcat gttccgaatc gccccagggg ggcgcctccc acgaggtgga
       61 aatgcaggtg aaagacggtc tgtcccgcgt gcgggccgac gttggtcacg agccgccacc
      121 ccgatcccag cccttcgccg ctcgcgacac gcgccgcgaa cgggaagagc tcgcccaaga
      181 gcgctccctg ttcgggagtg agctccgcgg cggaggcggc gatgtggcgc ttggggatga
      241 tcaggatatg cgtcggagcc tggggcgcga tgtcgcggaa cgcgaggagg tcggccgagt
      301 cggccacctt tttcgaaggg atctcgccgc tgacgatgcg gcagaagaga cagtcgctca
      361 tggtctccgc gcccggcccc ggggcggcgc ggaaaacgca gcgcccgcgg gccgtacggc
      421 tgagaccgta ctaagaggcc cgcgggcgtg ccaagcgatt ttcggttggg tcagtggttg
      481 tggccgcgtc ccctgccgtg tccaccctgg tcggagccct ggtcccggct gggcgccgat
      541 ccctggtcgg agttgccgcg gtcctgccgg ccccgccgcc gctcctgctg ctgttgcggc
      601 tgctcttgct gctgctgctg cacctgctgc tggcgacgct cctcctgacg cgcgcgagcg
      661 tcctgctgtt gctgctggcg ctgataccga tcctgctccg cctgctgacg cgcctgctgg
      721 cgctcgtagc gcgcctggtc cgcctgctgg cgctcatagc gcgcctgttc cgcctgctgg
      781 cgggcttgat cgcgttgcgc ctgctgatcg gcgcgcgcgc ggccctcggc ctcggcgcgg
      841 tcccgacgac gctgctcgga gcggccgcgg tcgagctgct gctgccggtc gtagcgctgt
      901 tgatcttgct gctggcggtc gtagcgctgt tgctgctgct ggcgctcgta tccgcgctgc
      961 cgctgctcgc gctcatcgac gcgctgctgc tggcgctcgt atccgcgctg ccgctgatcg
     1021 cggtcgaagc gctgctgctg ccggatcgcc cgctcttcac gggcgttccg tgcgcgctgc
     1081 tcgcgcatct gatcctgccg gccctgaccc atgcgggccg gacgctcgaa gtcgccctgc
     1141 cggtcgaccg gcgctccgcg ctcgatccga ccgcgcgcct gcgtctcgcc gccgcgtccc
     1201 ggaggcacgc gttgcccgcg ttccagccgg ccgccgcgaa cccgcatctc gcgctcgaag
     1261 cgatcgcgac gcgtgcgcat ctcgctggtc aggcgttcgc cgccggcgcg tccgcgcccg
     1321 ccgccgcgcg aaagcacggg catcggcgag acgagcgtcc tgccgcgcac catgtcgcgc
     1381 cggaggccgc tctcgcgggt cgtcaggcgg tattgcggaa tgtcacgacc cgtggcgcgc
     1441 tcgatcagcg ccggccggag tccgcgctcg gccacgcgcc cgctcaccga cgcgtaacgc
     1501 gtcagatccc gggtctcggt gtagatcctc cgcgtgtcgc ggagcggcgc cacgtaggtg
     1561 cgaagccgcg tgctggtgag ccggctctcc ggaacgaagc accagcgcga cggggcgacg
     1621 aaatcgtcgg cgtagcgaat gccgaacgaa acgtcccagg tcgcctcggg cggaaggggc
     1681 gcccagccgg catagccgcc gccgtagcgc cacgacaccc acgcgggcgc ccagacgtcg
     1741 tccggaaccc acatccagcg gttcgacgcg tcacaagccc agcggccgta gtggtacgga
     1801 tccgcgcccc agggatcgtc ggagacccag gcccatcccc aatcggtgta gacccagtat
     1861 ccgttcaggt aggggcgcca tccggccgaa acggcctcgg ggtaccagac ccagccgtac
     1921 cgcggatcgt cgtaccagtc gccgtaggga tccaactcgt cgtagaaata ggagacggtg
     1981 accgggtagc cgtactcgtc gtcaccatag tcgtcatagt cgtcgtcgta gccgccgtac
     2041 gaagaggcca ggtagtagtc gtacgacgga tcgcggccgt aggaatacgc ccagcgcccg
     2101 taatagtcgg tgccgccccg acggtagtcg cgccccgtcc tggaggcgta cgttccgctg
     2161 gaagccgcgc ggccgctcga agcgccaccg gcgtcgccgt acgtatcgga aggcggcccc
     2221 gcgtaggggt agtcgtcctg ggagagctgg tcgcctcgtc gcgacgagca ccccgcgaga
     2281 acgacggccg ccgccatcgt catccagatc gctgttcttc gaatcaccga aatgctcctt
     2341 cctgtcggaa cgggggtctc actgtctctc gtttcctaca ccgacccgcg ccgcccgtgg
     2401 cgggccgcgg gaccttccgt tgcgggaaaa gggcttacgg acggcccccg cgctacaccc
     2461 atccctctcc tatgctagat tcgccatcca gtcctacgat gcaggggtaa tgccaaggag
     2521 ggcgggtggt gggtaagaag atcgtgatcg ttggcggagg cgtctccggc ctggccacgg
     2581 cctggtactg cctggagcgg ggacacgacg tcaccatcct cgagcagggg ccggaacatc
     2641 acgactgctg ctcgctcggc aacgccggct tcatctcccc gagccacttc atcccgctcg
     2701 cgtcgccggg catcgtgggg aaggcgctcc gctggctcgg cgatcccgag agccccttct
     2761 gggtgcgtcc ccggctcgat ccctcgttcc tcgcctggtc ctaccgcttc tggagagcct
     2821 cgcggccgga gcgcgcgcgg cgcgcgggcc ccctccttcg cgatttgaat ctcgcgagcc
     2881 gctcactctt cgaggagctg gcccgccgta ccgggaacga cttcggcctc accgaagagg
     2941 ggcttctcct tctcttccgc tcggaggcgg cgctcgacgt ggagcgccgc cacgcggcgg
     3001 cggcccgcga gctcggcctg gaggccgtgg tcctcgaccg cgccggcgtg gcggcgctgg
     3061 aacccggcct cacgctggac gtgaccggcg gggtgcacta cccgatggac gcgcacgtga
     3121 cgccgcagcg cttctgcgcg acgctcgcgc gcatcgtgcg cgagcggggc ggcgacttcg
     3181 cctgggaatc gggcgtgacc gggtggcgcg cttcgggacg gagggtcgaa gcggcgatca
     3241 cgccccgcgg cgagcatcgc ggcgacgagt tcgtcgtggc cgcgggatcc tcgtcgcgca
     3301 gcgtgctccg gccgctgggc gtgccgatcg cgctcgagcc gggcaaggga tacagcctca
     3361 cgctgcccat cccgcccgag cggccgcgcc gctcattgct cctgcaggag gcgcgcgtcg
     3421 cgatcacccc catggggagc acggtccggg tcggcggcac gatggagctg ggcgcgggca
     3481 cgctgggggt caatccgccg cgcatccggg gcatcgtgcg ctccatgggg cggtacctgc
     3541 ctgcgttcac cccggaccgc ttcgcgggga tcgagccctg gagcgggctt cgcccctgca
     3601 cgccggacgg gctcccgtac atcggccgct ccgcgcgcct cgacaatctc ctcgtcgcca
     3661 cgggacacgc gatgctcgga ctgtcgctcg gcccggtgac cggctctttg atcgcggcgc
     3721 tcgtctccaa cgagaggccc gcgatcgaca tcgcgcccct tcgtccggag cggttcggat
     3781 gaaccggttc gggaaggcga tccgctccga gtggctgctc gatcccgcgt acgtctacct
     3841 gaatcacggc acggtcggcg cgccgccgcg ccgcgcgttc gaggcgcagg agcggatccg
     3901 ccgcgaagtg gagcgccaac cggcgcgctt cctgctgcgt gagctttccg ccatcgcggt
     3961 ggggggcccg cgcgcggagc cgccgcggat gcgggcggcc gccgagcgcg tcgcccggtt
     4021 cctgaacgcc gaggggaagg acctcgtctt cgtggacaac gccacgacgg cgatcaacgc
     4081 ggtgctccgc tccttcccgt tccgggcggg cgacgaaatc gtgatctccg accacgccta
     4141 cggcgccatc cgaaacgccg ccgcgttccg tgcgcgggaa accggggcgg tggtgcggga
     4201 cgtcgcgctt ccaggccctc cgttcgaggc ggcgcgcatc gccgaggcct acgcggccgc
     4261 gctctcgccg cgcgcgcggc tcctgatcgt ggatcacgtc acctcggaga gcgccctgat
     4321 cctcccgatc gagacgatcg tcgcggaggc caaggcgcgc ggcgtgcgcg tcctggtgga
     4381 cg
//
LOCUS       DASSLZ010000104         5572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_155877, whole genome shotgun sequence.
ACCESSION   DASSLZ010000104 DASSLZ010000000
VERSION     DASSLZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155877"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFM00_03885"
     CDS             complement(<1..563)
                     /locus_tag="VFM00_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497417.1"
                     /translation="MRVLVLTSRMPYPPVGGDRFRVYHMMRAAAEAGHAVHLLSFDTR
                     RQSEDGLAPLRRTLRSVDTVPLPRPLSWLRAAQAIPGRFPLQAAYYRSARMRERVDQA
                     IRRVRPHVVLTHLFRMAPYALPHLDHGRARWVLDLTDVISAGIERSLPFRSGADRWLY
                     QIEGPRIRDYEATVAPRFHECWVISQA"
     gene            628..2007
                     /locus_tag="VFM00_03890"
     CDS             628..2007
                     /locus_tag="VFM00_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497418.1"
                     /translation="MSGTERLATWSRRLRRFAWGAGPALIYAPLGILRNKWLASTLSA
                     PGLGVLAQVLSAMNWLGQAAGLGLGLPVTRLVGAARAADDSARERAVMRTALRLALGT
                     SGLVAVLVLIAAPILARALLGSAAYAPLFRIASIGIVGLAAAGTLLALAAGRGDIRPP
                     ITIAALGALPATALTFLLVPRWGLAGAMIAAAILYPAGCLAALAVHRRGYRTAFVGGI
                     AKPGEPAWAMARVGAAGLALGLLDQGVLLVLRAHYVRTYGPAANGFLQAALALSQLTG
                     SLFYTYLASYAFGTLSAAVAAAGPGRGAAAAGAYTRRQGPAIFALAALLFGIGMLIST
                     PLLRLLFSHRFDPARPLMAWSLLGEYGRVGMQVLLLGALPVGGLRLYAPVAIAFPVAL
                     AASYAAFTATGSGPLSLPRAYAAAGLIAAIAAALIMGRRGVTLGGREIGVLAGGALLL
                     AAIASQVLR"
     gene            2055..3377
                     /locus_tag="VFM00_03895"
     CDS             2055..3377
                     /locus_tag="VFM00_03895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7497419.1"
                     /translation="MTPLRHALERAAGLARRARARLFGSGLARDVNAAWRPAGARGPR
                     RALLLHVVELFRTKPGRHAHQNVGQVHELARALAERGYALDVADWFERRPVLRGAYDL
                     VIDLHPREPALYDAHLAANAKRLSYIGGMNPSWSNAAERRRLEDLRARRGVALKPRRQ
                     VPPFPPGRFESFDAMLTFAGTVAIRTFDEFRLPPVHRLVNNGYEDLVPTPPDRRDPRC
                     FLFLASLGQVHKGLDLLLEVFATRPELTLIVLSRFRQEPDFVDAYRRELFETPNIRAI
                     GFLDVDAPAFRDAQERAGWLILPSCAEAQCGAITVALSLGIPCVGSEACDIDNQEVIR
                     LPGTDLPTLGAMVDDLARRSSSEVRERSEASLALARRAYTRRDYARSIRAALDAVLEG
                     ALAPRPSHAGSAAGRPHGCFRRPFWDGTRRGTSSAHGAGSRRPRNRPG"
     gene            3374..4039
                     /locus_tag="VFM00_03900"
     CDS             3374..4039
                     /locus_tag="VFM00_03900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7497420.1"
                     /translation="MKEAYASPIAAADTGVLQRNLATRRIVATFLHLAGVREEPCLDY
                     AGGYGLFTRLMRDAGFSFYWCDPYATNLFARGFEWNPAIGTPRVVTAFEVLEHWARPL
                     EEFRQIAALGPEWIVTSTELHPGVRPDPAWPYLVPETGQHVAFYRPDTLRLLGREAGY
                     PHARIGPAYQVFGRSPVPAAAWWLAVRLGGALEPVVRRLRPTLTLADSERVRASARGE
                     DPR"
     gene            4039..5136
                     /locus_tag="VFM00_03905"
     CDS             4039..5136
                     /locus_tag="VFM00_03905"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET7497421.1"
                     /translation="MRPVVFTGDIFRIQSRGGITRYFRETIARLSRPVEVLAGWHQSP
                     ALRTLSVPVREAAFVPHRRGLTRPRALLNAAWDRNHLHEAARRGAIFHPTYYRDPRPL
                     PRTNPVVATVWDMTHERFAGLFRRRFWQAADSARWKRALCARADRIVCISEATRRDLL
                     ASIDVPEAKLHVIHAGAPDWSGVAARPMAGIPLPFFLWVGDRHGYKNFLPALEAWAAS
                     AAGRATALLCVGGGPLNAEECRAAARCGVLERVLHRAADDGELLWAYERAAGLLYLSR
                     SEGFGLPVLEAMSLGCPIVGSPAAALPEVVGTLAILADPEDRESMAAAIERCLERGRT
                     PEGEAALRARAALFTWDRCAREHERVYEALD"
     gene            complement(5118..>5572)
                     /locus_tag="VFM00_03910"
     CDS             complement(5118..>5572)
                     /locus_tag="VFM00_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497422.1"
                     /translation="REIVGGPVSLGRLHLQNRCGHQMIFCRRSVFDAAGTFDLRFAIA
                     ADYDWAIRVFQRSEITKRFVPEVAASMGAGGVSERRYVRLLRERGRIVRERFGSGAAV
                     EFALYAGAVEMPRLALRRLLGAVGMLRAARLVRRAVRGAPPGTQSSAS"
BASE COUNT          682 a         2110 c         1983 g          797 t
ORIGIN      
        1 tccgcctggc tgatcaccca gcactcgtgg aaccggggcg ccacggtggc ctcgtagtcg
       61 cggatgcgcg gcccctcgat ctggtagagc catcggtcgg cgccgctccg gaacggaagc
      121 gagcgctcga tccccgcgga gatgacgtcg gtgaggtcga ggacccagcg ggcgcgcccg
      181 tggtccaggt gcggcagggc gtagggcgcc atgcggaaga gatgcgtcag gaccacgtgg
      241 ggccgcacgc ggcggatcgc ctggtccacg cgctcgcgca tgcgcgcgga gcggtagtag
      301 gccgcctgga gcggaaagcg cccggggatc gcctgcgccg cgcgcagcca cgagagcgga
      361 cgcggcagcg gcaccgtgtc cacgctccgg agcgtgcgcc ggagcggagc gagaccgtcc
      421 tcgctctggc gccgcgtgtc gaacgaaagg aggtggacgg catgcccggc ttccgccgcc
      481 gcgcgcatca tgtgatagac gcggaaccgg tcgcctccga ccggcgggta gggcatgcgc
      541 gacgtgagga cgaggacgcg catagaggcc gcagtatacc acgcgtgaaa ttgacaccgg
      601 cagcgccgtc cccgagactc cgcccccatg tccgggaccg agcggctcgc cacgtggagc
      661 aggcgcctcc ggcgcttcgc gtggggcgcc ggacctgcgt tgatctacgc accgctcggt
      721 attctgcgca acaagtggct cgcctcgacc ctgtccgcgc ccgggctggg cgtgctggcc
      781 caggtcctct cggccatgaa ctggctgggt caggccgccg ggctcgggct gggcctgccg
      841 gtcacgcgcc tggtcggcgc ggcgcgcgcg gcggacgaca gtgcgcgcga gcgcgcggtc
      901 atgcgcacgg cgctccggct cgcgctcggg acatcggggc tggtcgcggt gctggtcctg
      961 atcgccgctc ccatcctggc gcgcgcactg ctgggctccg cggcctacgc cccgctcttc
     1021 cgcatcgctt cgatcgggat cgtcggactc gccgccgcgg gaacgctcct cgcgctggcc
     1081 gccggccggg gcgacatccg cccgccgatc acgatcgcgg cactcggggc cctccccgcg
     1141 accgcgctga cctttctcct cgtcccccga tggggactgg cgggggcgat gatcgcggcc
     1201 gccatactct acccggccgg atgcctggcc gcgctcgccg tccaccgccg cgggtaccgg
     1261 accgcgttcg tcggcggcat cgcgaagccg ggcgagcccg cgtgggcgat ggcgcgggtc
     1321 ggcgccgcgg gcttggcgct cgggctgctg gaccagggcg tcctcctcgt tctccgggcg
     1381 cactacgtgc ggacctatgg gcccgccgcg aacgggttcc tccaggccgc cctcgcgctg
     1441 agccagctga ccggatcgct cttctacacc tacctcgcga gctacgcctt cggcacgctg
     1501 agcgccgcgg tcgcggcggc cggccccggc cgcggcgcgg ccgccgcggg cgcctacacg
     1561 cggcgccagg gtcccgccat cttcgccctc gccgcgctgc tcttcgggat cggcatgctg
     1621 atctccacac ccctgctgcg gctcctcttc tcgcaccggt tcgatcccgc gcgtccgctc
     1681 atggcctggt cgctgctcgg cgagtacggg cgcgtcggca tgcaggtcct cctcctcggc
     1741 gcgctgccgg tcggcgggct gcgcctctac gcgccggtcg cgatcgcctt cccggtcgcg
     1801 ctcgccgcga gctacgccgc attcaccgcg acgggctccg gcccgctgag ccttccccgc
     1861 gcctacgccg cggccggcct gatcgccgcg atcgccgccg ccctgatcat gggccgccgc
     1921 ggcgtgaccc tcggcggccg cgaaatcggc gtcctcgccg gcggggcgct tctcctggcc
     1981 gcgatcgcga gccaggtgct ccggtagcgg tatactcggc cggcttggac gctcccggtt
     2041 cgagatcagg tttcgtgacg ccgcttcggc acgcgctcga gcgcgcggcc ggtctcgccc
     2101 gtcgcgcgcg agcgcgcctc ttcggctcgg gcctcgcgcg tgacgtgaac gccgcgtggc
     2161 ggccggccgg cgcccggggg ccgcgacgcg cgctccttct gcacgtggtc gagctcttcc
     2221 gcacgaagcc ggggcgccac gcgcatcaga acgtgggcca ggtgcacgag ctggcgcgcg
     2281 cgctcgccga gcgcggctac gccctcgacg tggcggactg gttcgagcga aggcccgtgc
     2341 tgcgcggcgc ctacgacctg gtgatcgatc tccatccgcg ggagccggcc ctgtacgacg
     2401 cgcatcttgc cgcgaacgcg aaacggctct cctacatcgg cggcatgaac ccgtcgtgga
     2461 gcaatgccgc cgagcgccgg cgcctcgagg acctgcgggc gcggcgcggc gtcgcgctta
     2521 agccgcggcg gcaggtgcct ccgtttccgc ccggccggtt cgagtcgttc gacgcgatgc
     2581 tcaccttcgc cgggaccgtg gcgatccgga ccttcgacga gttccgcctt ccgccggttc
     2641 accgcctggt gaacaacggg tatgaggacc tggtcccgac gccccccgac cgccgcgatc
     2701 cgcgctgctt cctcttcctc gcgagcctgg ggcaggtgca caaaggcctc gacctgctcc
     2761 tcgaggtgtt cgccacgcgg cccgagctca ccctgatcgt gctctcccgc ttccgccagg
     2821 agcccgactt cgtcgacgcc taccggcgcg agctcttcga gacgcccaac atccgcgcga
     2881 tcggattcct ggacgtggac gcgcccgcgt tccgggacgc gcaggagcgc gcgggctggc
     2941 tcatcctccc ctcgtgcgcc gaggcgcaat gcggggccat caccgtggcc ctctcgctcg
     3001 gaatcccctg cgtcggaagc gaggcctgcg acatcgataa tcaagaagtg atccgcctcc
     3061 ccggcaccga tctccccacg ctgggcgcga tggtggacga cttggcccgg cgcagtagct
     3121 ccgaagtccg cgagcgctcc gaggcctcgc tcgcgctggc gcgccgcgcc tacacgcgcc
     3181 gcgactacgc ccgctcgatc cgcgcggcgc tggacgccgt gctcgagggc gcattagcgc
     3241 cgcgaccatc gcatgccggc tctgcggcgg gccggccgca cggctgtttt cgaagaccgt
     3301 tctgggacgg tacgaggcgg ggtacttcca gtgctcacgg tgcgggctca cgcagaccga
     3361 ggaaccgacc tggttgaaag aggcgtacgc ctcgccgatc gcggcggccg acaccggcgt
     3421 gctccagcgg aatctcgcga cgcgccggat cgtcgcgacc ttcctccacc tggccggcgt
     3481 gcgggaagag ccctgcctgg actacgccgg cggatacggg ctcttcacgc ggctcatgcg
     3541 cgacgcgggg ttctcgttct attggtgcga cccgtacgcc acgaacctct tcgcgcgagg
     3601 cttcgagtgg aatcccgcga tcgggacgcc gcgggtcgtg accgcgttcg aggtgctgga
     3661 gcactgggcg cgcccgctcg aggaatttcg ccagatcgcc gcgctcggcc ccgaatggat
     3721 cgtcacgagc acggagcttc atcccggcgt acgccccgat ccggcgtggc cctacctggt
     3781 ccccgagacc ggccagcacg tcgcgttcta ccgtcccgac acgctgcggc tcctggggcg
     3841 cgaggccgga tacccgcacg cccggatcgg acccgcatac caggtcttcg ggcggagccc
     3901 cgtcccggcg gcggcgtggt ggctcgcggt ccgcctgggc ggagcgctgg agccggtggt
     3961 gcgccggctc cgccccacgc tcacgctggc cgacagcgag cgggtccgcg cctcggcgcg
     4021 cggcgaagat ccgcgctgat gcgcccggtc gtcttcacgg gcgacatctt ccgcattcag
     4081 agccggggcg gcatcacccg atacttcagg gagacgatcg cgcgcctgtc ccgtcccgtg
     4141 gaggtgctgg ccgggtggca tcagtcgccg gcgctccgga cgctctccgt cccggtccgc
     4201 gaagcggcgt tcgttccgca ccgaagaggg ctcacgcggc cgcgcgcgct cctgaacgcg
     4261 gcctgggacc gcaaccatct ccacgaagcc gcgcgccgcg gcgcgatctt ccaccccacg
     4321 tactaccgcg acccccgccc gctgccgcgg accaaccccg tcgtcgcgac cgtctgggac
     4381 atgacccacg agcgcttcgc gggcctcttc cgccggcgct tctggcaggc cgcggactcc
     4441 gcgcgctgga agcgcgcgct ctgcgcgcgc gccgaccgga tcgtctgcat ctccgaagcg
     4501 acgcggcgcg acctgctcgc ttccatcgac gtgcccgagg cgaagcttca cgtgatccat
     4561 gccggggcac ccgactggtc gggcgtcgcg gccaggccga tggccgggat tccccttccc
     4621 ttcttcctct gggtcggaga ccggcatggg tacaagaact tcctcccggc cctggaggcg
     4681 tgggcggcga gcgccgcggg gcgcgcgacc gcgctcctct gcgtcggagg ggggccgctg
     4741 aacgctgagg agtgccgtgc cgccgcgcgg tgcggcgtcc tggagcgcgt gctccatcgc
     4801 gcggccgacg acggggagct cttgtgggcg tacgagcgcg cggccgggct tctctatctt
     4861 tcgcgctccg agggcttcgg cctgccggtg ctggaggcga tgtcgctggg atgcccgatc
     4921 gtgggatcgc ccgcggcggc gctccccgag gtcgtcggca cactggcgat cctggcggac
     4981 cccgaggacc gggagagcat ggccgcggca atcgagcgct gcctggagcg ggggcgaacg
     5041 cccgagggcg aagcggcgct ccgcgcccgc gcggccctct tcacgtggga ccgctgcgcg
     5101 cgcgagcacg aacgggtcta cgaagcgctg gactgagtcc cgggcggcgc gccgcgaacg
     5161 gcgcggcgaa cgaggcgcgc ggcgcgcagc atccccaccg cgccgagcag gcgccgcagg
     5221 gcgagccggg gcatctccac cgcgcccgca tagagcgcga actccaccgc ggcgccggac
     5281 ccgaatcgct cgcggacgat gcggccgcgc tcgcgaagaa gccgtacgta gcgccgctcg
     5341 ctcacgccgc ccgcccccat cgaggcggcg acctccggca cgaagcgctt cgtgatctcc
     5401 gaccgctgga acacgcggat cgcccagtcg tagtcggccg cgatcgcgaa gcggaggtcg
     5461 aaggtcccgg cggcgtcgaa cacggagcgg cggcagaaga tcatctggtg tccgcaccgg
     5521 ttctggaggt ggagccgccc cagcgagacc ggtcccccca cgatctcccg cg
//
LOCUS       DASSLZ010000105         4773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_543267, whole genome shotgun sequence.
ACCESSION   DASSLZ010000105 DASSLZ010000000
VERSION     DASSLZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543267"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..772)
                     /locus_tag="VFM00_03915"
     CDS             complement(89..772)
                     /locus_tag="VFM00_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HET7497423.1"
                     /translation="MTRTEMRFTASHSAGEVSAILDRPDDARRLYVFAHGAGAGMRHR
                     FMEAASEALGAKGIATFRYQFAYMEKRSRRPDPQPVLLATVRAAVAMARKATGDLPML
                     AGGKSMGGRMTSLASAEEPLEGVEGLVFFGFPLHPAGQPGTKRADHLEAVAVPMIFLQ
                     GTRDTLANLELVEPVVARLPHATLHVVEGADHSFEVPRKTGRSLADTLDDLAATIAGW
                     ADARDKERP"
     gene            complement(807..3446)
                     /gene="ligD"
                     /locus_tag="VFM00_03920"
     CDS             complement(807..3446)
                     /gene="ligD"
                     /locus_tag="VFM00_03920"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HET7497424.1"
                     /translation="MSPTGRSQLEKYREKRDPSRTPEPFGEAAAAEAPAGPGPRFVVQ
                     KHWARAMHYDLRLELDGTLKSWAVPKGPSTHVEEKRLAVHVEDHPLEYANFEGVIPSG
                     NYGAGSVIVWDRGWFRSFKPEDPLEQYERGKLELELFGFKLRGRWTLVRMGKKDKEWL
                     LLKKADDAATKEEAIDRYPESVISGLTVEEMRDVAGTLEALRERVHALGAPRGELDPK
                     EVPLTLATPESAPPKGDDWSFEIKYDGVRVRALRDEDRVVLIGRSGEDVTKRYPEIAG
                     ALRELAVPRFLADGEIIAEDERGHPSFQRLQARMGLTKPRDVAAAMIQVPVRAVFFDL
                     LGLEGHDLRGLPLRVRQELLARVLPPLATAQRTTHLVGHGEAFFAAAGQMGLEGVVAK
                     RLESKYAGRRTPDWVKVKCDRRQEFVIGGYTEPQGSRAGFGALHVGVYEGSKLIYVTK
                     VGTGFDDAKIGAIRKLLDPLARETTPFDEGTPTGRGHRWVQPRLVCEVRFTEWTRDGG
                     LRHPTFMGMRDDKRPEDVHREDRARDDDNAGTLGEDAALPEEAMPVPEASVEGVEGAK
                     PTSSARRKPLTPGARAAAAQTVHVTNPKKVFWPDGTTKGDLVAYYETVAPLLLPYLRD
                     RPVVLTRYPDGIEGKSFFQKDAPVYVPDWVRTVVVPAGGGERDIRYFVIDDLESLRYV
                     ANLGTIPLHVWSARASSLEHPDWMVLDLDPKEAPFVHVVQVARALKTILDRLELPSSI
                     KTSGATGLHVLVPMGGRYTHEESRTFARLLAMLTVEAVPEISTVTRAIGGRSGKVYVD
                     FGQNGRGNTIVSPYSVRPRAGASASCPLRWDEVTPRLDPAAFTIRTIPKRFETMEDPM
                     TTVLGPGVDMAAAIAAIQKDMKS"
     gene            3595..4461
                     /locus_tag="VFM00_03925"
     CDS             3595..4461
                     /locus_tag="VFM00_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET7497425.1"
                     /translation="MPPHSIGSGTLSFGLVSIPVKMFTAASSGGVSFNQLHEKCGGRI
                     KQQLHCPACNEIVDRSALVKGYEFAKDQYVRFTDEELKRLEAEASPTIDIAEFVPLDQ
                     VDPIYFEKSYYLGPDKGGDKAYRLLCEAMEQAGQVALAKYVMRGKESLVLIRSSQGGL
                     MLHAMYFADEIRDFGEIDKGKEAAIKPNELALAQKLIQELSNPEFKPDTYHDEYRGRV
                     LAVVESKVEGKEITAGAPQVARTQVIDLMEALKQSLESRASSEEPAKAKKPAAKAKRA
                     AAPVKESKRAAR"
     gene            4532..>4773
                     /locus_tag="VFM00_03930"
     CDS             4532..>4773
                     /locus_tag="VFM00_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7497426.1"
                     /translation="MDAVTTYSTRDVARILHVSEPRVRTWARMGGIVPKPGTDGALEF
                     TFQELLLLRTTRGLLDAGVPPRRVKRIWSTLKQQLS"
BASE COUNT          742 a         1684 c         1586 g          761 t
ORIGIN      
        1 cgcagcatgt ccttggccgc aagcttctcg gggtagaagt gacccttcca ctctttgtac
       61 gaccagccgc tggttcccgc tcggatcttc atggtcgctc cttgtcccgc gcatccgccc
      121 agcccgcgat cgtcgcggcc aggtcgtcca gcgtgtcggc caggctccgg cccgtctttc
      181 tgggcacctc gaacgagtgg tccgcgccct cgaccacgtg cagcgtcgcg tgcgggagcc
      241 gcgccacgac cggctccacg agctccaggt tcgcgagcgt gtcgcgcgtc ccctgcagaa
      301 agatcatcgg taccgcgacg gcctccagat gatcggcgcg cttggtcccc ggctgcccgg
      361 ccgggtggag tggaaagccg aagaatacca gcccctcgac gccttccagc ggctcctcgg
      421 ccgaagcgag cgacgtcatg cgcccgccca tcgacttgcc gccggccagc atcggaaggt
      481 caccggttgc cttccgggcc atcgcgaccg cggcacgcac cgtggcgaga aggaccggct
      541 gcgggtcggg acgccggctc cgcttctcca tgtaggcgaa ctgataccgg aaggtcgcga
      601 tccccttcgc gccgagggct tccgaagctg cctccatgaa gcgatggcgc atccccgcgc
      661 ccgcgccgtg ggcgaagacg tacagccgcc gcgcatcgtc cggccggtcg aggatggccg
      721 agacctctcc cgcggagtgg ctcgcggtga agcgcatctc ggtccgggtc acggcctcat
      781 gcccctgtgg atcgcggcga ccgcagtcat gacttcatgt ccttctggat cgccgcgatc
      841 gcggcggcca tatccacgcc ggggcccagc acagtcgtca tcggatcttc catcgtctcg
      901 aagcgcttcg ggatcgtccg gatggtgaac gccgcggggt cgagccgcgg cgtcacctcg
      961 tcccagcgga gcgggcagga cgccgacgcg ccggcgcgcg ggcgcaccga gtagggcgac
     1021 acgatcgtgt tcccgcggcc gttctggcca aagtccacgt agaccttccc gcttcggccg
     1081 ccgatggcgc gcgtgaccgt ggagatctcg ggcaccgcct ccacggtgag catcgcgagc
     1141 aagcgcgcga acgtgcgcga ctcctcgtgc gtgtagcggc cccccatcgg caccagcacg
     1201 tggaggccgg tggcgcccga ggtcttgatc gaggagggca gctccagccg gtccaggatc
     1261 gtcttcagcg cgcgcgcgac ctgcacgacg tgaacgaacg gcgcttcctt gggatcgaga
     1321 tcgagcacca tccagtcggg atgctccagc gagctcgcgc gcgcgctcca gacgtggagc
     1381 ggaatcgttc ccaggttcgc cacgtagcgc agcgactcga ggtcgtcgat cacgaagtag
     1441 cggatgtccc gctcgccccc gccggcgggc acgaccacgg tgcgcaccca gtcgggcacg
     1501 tacaccgggg cgtccttctg gaagaacgat ttcccttcaa tgccatcggg gtatcgggtc
     1561 agcacgaccg gtcgatcgcg aagatacgga agcaggagcg gcgcgacggt ctcgtagtag
     1621 gcgacgagat cgcccttggt ggtcccgtcg ggccagaaga ccttcttggg attcgtgacg
     1681 tgcaccgtct gcgccgcggc cgcgcgggcg ccgggcgtga gcggcttgcg ccgggcggag
     1741 gacgttggct tggcgccttc aacgccctcg acggacgctt ccggtacggg cattgcctcc
     1801 tccggaagcg cggcgtcctc gccgagagtg ccggcgttgt cgtcgtcgcg cgcacgatcc
     1861 tcccgatgca cgtcctccgg cctcttgtcg tcgcgcattc ccatgaacgt gggatggcgc
     1921 agcccgccgt cgcgcgtcca ctccgtgaag cgcacctcgc acaccagtcg cggctggacc
     1981 cagcgatgcc ctcgccctgt cggcgtcccc tcgtcgaagg gggtcgtctc gcgcgcgagg
     2041 ggatcgagga gcttccggat cgcgccgatc ttcgcgtcat cgaacccggt gcccaccttc
     2101 gtcacgtaga tcagcttcga gccctcgtac acgcccacgt gcaaggcgcc gaacccggcg
     2161 cgcgaaccct gcggctcggt gtagccgccg atcacgaact cctggcgccg gtcgcacttc
     2221 accttcaccc agtcgggcgt gcgccgcccc gcgtacttgc tctccaggcg cttggcgacg
     2281 accccttcca gccccatctg cccggccgcc gcgaagaagg cttccccatg gccgacgaga
     2341 tgggtcgtgc gctgggcggt ggccagaggg ggcagcaccc gcgcgagcag ctcctgacgc
     2401 acccggagcg gcagtccgcg caggtcgtgc ccttccagtc cgagcaggtc gaagaacacg
     2461 gcgcgcacgg gaacctggat catcgccgcc gcgacgtcgc ggggcttggt gagtcccatc
     2521 cgcgcctgga gccgctgaaa gctggggtgc ccgcgctcgt cctcggcgat gatctcgcca
     2581 tcggccagga agcgcggcac ggcgagctcg cgcagcgcgc cggcgatctc cgggtatcgt
     2641 ttcgtcacgt cctcgccgct ccggccgatc aacacgacgc ggtcctcatc gcgcagcgcg
     2701 cgcacgcgca cgccgtcgta cttgatctcg aacgaccagt cgtccccttt cggcggcgcg
     2761 gactcgggag tagcgagcgt gagcggaacc tccttggggt cgagctcgcc gcgcggcgcg
     2821 cccagcgcgt gaacccgctc gcgcagcgcc tcgagcgtgc ccgccacgtc ccgcatctcc
     2881 tcgacggtaa gccccgagat caccgactcc ggatagcggt cgatcgcctc ctccttggtg
     2941 gccgcgtcgt ccgccttctt caagagcagc cactccttgt ccttcttccc catccggacc
     3001 agggtccagc gcccgcgcag cttgaacccg aacagctcca gctccagctt gccgcgctcg
     3061 tactgctcca gcggatcctc gggcttgaac gagcggaacc agccgcggtc ccagacgatg
     3121 accgaccccg cgccatagtt gcccgagggg atcaccccct cgaagttggc gtattccagc
     3181 ggatggtcct cgacatggac cgcgaggcgc ttctcttcga cgtgggtgga gggccccttg
     3241 gggaccgccc agctcttcag ggtcccgtcc agctcgaggc ggaggtcgta atgcatcgcg
     3301 cgcgcccagt gcttctgcac gacgaagcgg gggccggggc cggccggggc ttccgcggcg
     3361 gcagcctccc cgaacggctc cggggtgcgg gaggggtccc gcttctcgcg gtacttctcg
     3421 agctgggagc ggccggtcgg gctcaaggat caggcgcctc gggctttcgg ataggcgcct
     3481 ctgccaagca cttgcggctc aagcacgcgc cctctggaac cgatgtttcg acagggagtt
     3541 gagtatagca gaggcgaatg ttagcctcgc ttaaccccac ggaggaatac aggtatgccc
     3601 ccacattcca tcggttcggg caccttgtcg ttcgggctgg tttcgatccc cgtcaagatg
     3661 ttcacggccg cctcttcggg cggcgtgtcg ttcaaccagc tccacgaaaa gtgcggcggc
     3721 aggatcaagc agcagctcca ctgccccgcc tgcaacgaga tcgtggaccg gtcggcgctg
     3781 gtcaagggct acgagttcgc caaggaccag tacgtccgct tcaccgacga ggagctgaag
     3841 cgcctggagg cggaggcctc gccgaccatc gacatcgccg agttcgttcc tctggaccag
     3901 gtcgacccga tctatttcga gaagtcctac tacctgggtc ccgacaaggg cggcgacaag
     3961 gcctaccggc tcctctgcga agccatggag caggcgggac aggtggcgct ggccaagtac
     4021 gtcatgcgcg gcaaggagag cctcgtccta atccgctcca gccagggcgg gctgatgctc
     4081 cacgccatgt acttcgccga cgagatccgc gacttcggcg agatcgacaa ggggaaggaa
     4141 gcggccatca agccgaacga gctggcgctg gcgcagaagc tgatccagga gctgtcgaat
     4201 ccggagttca agccggacac gtaccatgac gagtatcgcg gccgggtcct agccgtcgtc
     4261 gaatcgaagg tcgagggcaa ggagatcacg gcgggagcgc cccaggtcgc gcggacgcag
     4321 gtcatcgatc tcatggaggc gctgaagcag agcctggagt cgcgcgcctc cagcgaggaa
     4381 ccggcgaagg ccaagaagcc cgcggccaag gcgaagcgcg ccgcggctcc ggttaaggag
     4441 agcaaacggg ccgcccgttg atgtagatta cctgccgggg gtctttcgga gcgcggtctg
     4501 gcggtaggga cgtcgtgaag ggcggtcggg catggatgcg gtgacgacct attccacgcg
     4561 cgacgtggcg cggattctcc acgtctcgga gccccgggtt cggacctggg cgcgcatggg
     4621 cggcatcgtc cccaagcccg gcaccgacgg cgccctcgaa ttcaccttcc aggagctcct
     4681 cctcctccgc accacgcgcg gcctgctcga tgcgggcgtt ccgccgcggc gcgtcaagcg
     4741 catctggtcc accctgaagc agcagctctc cga
//
LOCUS       DASSLZ010000106         5293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1176574, whole genome shotgun sequence.
ACCESSION   DASSLZ010000106 DASSLZ010000000
VERSION     DASSLZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176574"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1909)
                     /locus_tag="VFM00_03935"
     CDS             complement(<1..1909)
                     /locus_tag="VFM00_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497427.1"
                     /translation="MSPLTSPGGRGRPDASSRFQAWLAVLGAAYAAALALASRSTSAS
                     LWGIHHAAFLDGTSRVLVLGAAALGAALLVLSAARGGRQRPAVPRSSKPRGPTLATIL
                     PLYALPLIGIAFWTFRARTYFLGDGIVWLANLTSGHFPRFSEPLSTEAWHGYTRGLHA
                     LGLVTDAASLALLSVLCGVITAVLLWLLALEMAPRPRGRIAAAALLLTLGVTQLYFGY
                     IESYPIVSVLVVAYLVAGARAARSERPEVLPGIALALVAAAHIVSLLLAPSYLWLVAR
                     MRTSLWRKALLLLAPAILAAGLFAWLRFSVADLLRPFHTMGTALQGGTGAFGFTARGF
                     LSARRWIDLLNLALLLAPVPLLVLGARLGAGWGIHGNVATSNRTLAFLVMAGAPGVLA
                     ALFLVVPGSPAQDWDLLAIILLPAAVAAVAASLPALERAPRPALAGVAILGLAGLAGF
                     VLVNADVGAGLRRFESLVAEDAALRPHERAYGNEKLATYWSDRGDYDRALIYARQAVG
                     AEPENPRYWVKAGGALVSLGRQDEAVPFFTEAIRRDPGRADAYYNLGICYAKAERYDE
                     AVGAFRSAVVLDRDRPDYRHNLGLMLYAAGKPDSARLVWTEVLRRWEGYPLTVRSMAR
                     RFGPDASAASRH"
     gene            complement(1957..3702)
                     /locus_tag="VFM00_03940"
     CDS             complement(1957..3702)
                     /locus_tag="VFM00_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7497428.1"
                     /translation="MGTVKRFVGVVLAVTAIYLPGTARVARAEWPRFGRPVVTANNGQ
                     QHAAVARDGAGGAIITWQDFRSPRVNTFAQHVLASGELDARWPVDGRSLLADPAALDS
                     ADGGQISQRIVSDGAGGAIVAWLDNRSPVTEIDVFAQHVLASGVVDPAWPANGAGLCV
                     VEGVQNSLVLAPDGVGGAIAAWLDVRPGGASADIFAQHILSSGTVDPRWPVNGIAVSA
                     APGAQELPVIAEDGAGGAFVAWEDARDPNNLDVFAEHVLSTGTLDPAWPVDGLPVGAT
                     IGSQGRPAIGFDGCHGAIVAWVDNRSGTADHIFAQHVILTGVDPAWPANGRGISGATA
                     EESRPILVTDGAGGAIVSWQAFNFNVAEYAQHVTAAGTVDPAWPAAGLRLSASELDET
                     QGAIAADDAGGAIVAWRENFDVIVQHVLASGTLDPQYGATGRPVVALPSGQGDPAIVA
                     SGGGGAVVAWTDTRKGTDVDIYAMQVAAIVTTGVPDSTPPGLALRRPGPNPARGSVTL
                     RFSLPAPAPARLTIFDASGRLVRVLASGAQSGGEHVLAWDLRDQAGHDVPSRVYFARL
                     ETEGHTLTEKLVTVR"
     gene            complement(3732..4250)
                     /locus_tag="VFM00_03945"
     CDS             complement(3732..4250)
                     /locus_tag="VFM00_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497429.1"
                     /translation="MNKTIVAAMASGMLLAAASGSLGQGTRHSFSFNAVNISGAPTGA
                     VTLAGGGVYDIGSGFQQGGGAFRCTQDITQGPLAGLRAGEGVRWATVTLVPSTGFRCG
                     SDAGEPLKTATTDGDTVVLQAIFFRQGDGNNPSFTAKVFVSASDEDPDAPGVQNVWIQ
                     GVGCAEASVNVR"
     gene            complement(4308..4682)
                     /locus_tag="VFM00_03950"
     CDS             complement(4308..4682)
                     /locus_tag="VFM00_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HET7497430.1"
                     /translation="MKAPFRVFRLLAVPVLILATATSCNSGKSNPTGPGTAGPELDSG
                     VLGPGAVYQHTFASAGSYAYHCIFHTPMRGTVQVNAAAADSIAHVSITSSTTTFPGAT
                     IRPGGSVVWTNNTQLDHTVTSD"
     gene            4932..>5293
                     /locus_tag="VFM00_03955"
     CDS             4932..>5293
                     /locus_tag="VFM00_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7497431.1"
                     /translation="MATGADHPRGRPRSEKARQAIFDAACDLFEEGGYPAATIEAIAA
                     RSGVAKTTIYRWWPNRPALVVEVLLRLAATAAPPPEAGLDPVRALRSELHRVAQAMDA
                     LPGRLLLSLLGEAQNDADV"
BASE COUNT          827 a         1845 c         1893 g          728 t
ORIGIN      
        1 cgtgccggct ggcggcgctc gcatccggcc cgaaccgccg agccatcgag cggacggtca
       61 ggggatagcc ctcccaccgc cgtaagacct ccgtccagac gagcctggcc gagtcgggtt
      121 tccctgccgc gtagagcatc aatccgagat tgtggcggta gtcggggcgg tcgcggtcga
      181 gcaccacggc ggaccggaac gcgcccaccg cctcgtcgta gcgctccgcc ttggcgtagc
      241 agattcccag gttgtagtag gcgtccgccc ggccggggtc gcggcggatg gcttcggtga
      301 agaacgggac cgcctcgtcc tggcggccca gcgacaccaa ggcgccgccc gccttgaccc
      361 agtagcgcgg attttccggc tcggctccca cggcttggcg cgcgtagatg agcgcccgat
      421 cgtagtcgcc gcgatccgac cagtaggtcg ccagcttctc gttgccgtag gcgcgctcgt
      481 gggggcgcag cgccgcgtct tccgccacca gggactcgaa acgccggagt cccgcgccca
      541 cgtcggcgtt caccagaacg aagccggcaa ggccggccag gccgaggatg gcgacgccgg
      601 cgagcgcggg acgtggagcc cgctcgagcg cgggcaggct cgccgcgacg gcggccaccg
      661 ccgcgggcag gagaatgatc gcgaggagat cccagtcctg cgcgggtgac ccgggcacca
      721 cgaggaacag cgcggcaagg acacccggcg ctccggccat gaccagaaac gcgagggtgc
      781 ggttcgacgt cgcgacattc ccatgaatcc cccaccccgc gcccaggcga gcgcccagga
      841 ccaggagcgg caccggcgcc agcagcagcg ccaggttcag gagatcgatc caccgccgcg
      901 ccgacaggaa cccgcgtgcg gtgaagccga acgcccctgt ccctccctgg agggcggttc
      961 ccatcgtatg aaacgggcgc aggagatcgg ccacgctgaa gcgcagccag gcgaagaggc
     1021 ccgcggcgag gatcgcgggc gcaaggagca ggagggcttt tcgccacagc gatgttcgca
     1081 ttcgcgccac gagccatagg tacgatggcg cgagcagcag cgagacgatg tgcgcggccg
     1141 ccaccaaggc cagcgcgatc cccggcagga cctccggtcg ctcggagcgc gcggcgcgcg
     1201 cgccggcgac gagatacgcg acgacgagca cgctcacgat ggggtagctc tcgatgtaac
     1261 cgaagtagag ctgggtgacg ccgagcgtga ggagcagcgc ggcggcggcg atccgcccgc
     1321 gcggcctcgg cgccatctcc agcgcgagca gccacaagag gaccgccgtg atcacgccgc
     1381 aaaggaccga cagcaaggcc agcgaggcgg catccgtcac cagccccagc gcatgaagcc
     1441 cccgcgtgta gccatgccag gcctcggtcg agagcggctc gctgaatcgt gggaagtgcc
     1501 ccgaggtgag attggcgagc cacacgattc cgtcgccgag aaaataggta cgcgcgcgga
     1561 aggtccagaa ggcgattccg atcaggggga gcgcatagag cggcaggatg gtcgcgagcg
     1621 taggaccacg cggcttcgag gatcgcggga ccgcggggcg ctgccggcca ccgcgcgccg
     1681 cggagaggac gaggagtgca gcacccagcg ccgcggcgcc caggaccaga acgcgcgacg
     1741 tcccgtcgag gaacgcggcg tgatgaatgc cccacaggct tgcggacgtg ctgcggctcg
     1801 caagcgccag ggccgcggcg taggcggcgc cgaggacagc gagccacgcc tggaaccgtg
     1861 aactcgcatc cggcctaccc cgccctccgg gggacgtgag gggggacatg ggccggacta
     1921 tagacccatg tccccctcac ggccagcgtg cgggccttag cgaaccgtca ccagcttctc
     1981 cgtgagcgtg tggccttccg tttcgaggcg cgcgaagtag accctggagg ggacgtcgtg
     2041 cccggcctgg tcgcgaagat cccacgccag gacgtgctcc cctccgctct gcgcgccgga
     2101 ggccagcacg cgcaccaacc ggccgctcgc gtcgaagatg gtcaggcgcg cgggcgccgg
     2161 agccggcaag gagaagcgca gcgtgaccga tccgcgcgcg ggattgggcc ccggccggcg
     2221 cagcgcgagc ccggggggcg tggagtcggg caccccggtc gtcacgatgg ccgcgacctg
     2281 catggcgtag atgtccacgt cggtcccctt ccgggtatcg gtccaggcca cgacggcgcc
     2341 gcctcccccg ctcgcgacga tggcgggatc gccctggccg ctggggagcg cgacgaccgg
     2401 tcggccggtg gcgccgtact ggggatccag ggtgcccgag gccagcacgt gctggacgat
     2461 gacgtcgaaa ttctccctcc aggcgacgat ggcgccgccc gcgtcgtcag cagcgatcgc
     2521 cccttgggtc tcgtcgagct cgctcgcgct gagccggagt ccggcggccg gccacgccgg
     2581 atccaccgtc cccgccgcgg tcacgtgctg ggcgtactcg gctacattga agttgaatgc
     2641 ctgccagctc acgatcgcgc cgccggcgcc gtcagtgacg aggatcggcc gcgactcctc
     2701 ggcggtggcg ccggagatcc cccggccgtt cgccggccac gccgggtcga caccggtgag
     2761 gatcacgtgc tgtgcaaaga tgtggtctgc cgtgccggag cgattgtcga cccacgcgac
     2821 gatggcgccg tggcatccgt cgaagccgat cgcggggcgc ccttggctgc cgatcgtggc
     2881 gcccacgggc agtccgtcga ccggccatgc gggatccagg gttcccgtgc tcaacacgtg
     2941 ttccgcgaac acgtcgaggt tgttcggatc ccgcgcgtct tcccacgcca cgaaggcgcc
     3001 gcccgcgccg tcctcggcga tgacgggcaa ctcctgcgct cccggagcgg cggagacggc
     3061 gatgccgttc acgggccacc gcgggtccac ggtgcccgaa gacaggatgt gctgcgcaaa
     3121 gatgtccgcc gaagcgccgc ccgggcggac gtccagccag gcggcgatcg cgccgcccac
     3181 tccgtccggc gcgagaacga ggctgttttg aaccccctcg acgacgcaga gaccggcgcc
     3241 gttcgcgggc cacgccgggt ccacgacacc cgacgcaagc acgtgctgcg cgaagacgtc
     3301 gatctcggtc accggactcc ggttgtccag ccatgcgacg atcgcgccgc cggctccgtc
     3361 ggacacgatg cgctgcgaga tctggcctcc gtcagcgctg tccagggccg ccggatccgc
     3421 gaggagggac cgcccgtcga ccggccagcg ggcatccagc tctcccgagg ccagcacgtg
     3481 ctgggcgaag gtgttgaccc tcgggcttcg gaaatcctgc caggtgatga tcgccccgcc
     3541 cgcgccgtcc cgggccaccg ccgcgtgctg ctggccgttg ttggccgtga cgaccgggcg
     3601 tccgaaccgg ggccattccg cccgggcgac gcgcgccgtt ccgggcaggt agatggcggt
     3661 cacggccaat acgacaccca cgaatctctt gacggtaccc atgacgggcc tcttttcctg
     3721 tcggaatgga actaacgaac gttgacggat gcctcggcgc aacccacccc ctggatccac
     3781 acgttctgga ccccgggcgc gtccgggtct tcgtccgacg ccgagacgaa caccttggcc
     3841 gtgaaggacg gattgttgcc gtcgccctgc cggaagaaga tcgcctgcag caccacggtg
     3901 tccccgtcgg tggtcgcggt cttcaagggc tcgcccgcgt cgctgccgca cctgaagccg
     3961 gtcgacggca ccagcgtcac ggtcgcccat cgaacgccct cgcccgcgcg aagtcccgca
     4021 agcggtccct gcgtgatgtc ctgggtgcag cggaacgcgc cgccgccctg ctggaatccc
     4081 gatccgatgt cgtacacccc gccgccggcg agggtcaccg cccccgtcgg cgcgccgctg
     4141 atgttcacgg cgttgaagga gaaggaatga cgggtgccct ggccgaggct gcccgaggcg
     4201 gcggcgagca gcattcccga tgccatcgcg gcgacgatcg tcttgttcat ggtcgtgtcc
     4261 tccggtcgtg ggtgtgctcg agcggctggc tcagagcggt cgatgcttca gtcgctggtg
     4321 acggtgtggt cgagctgcgt attgttcgtc cagacgacgc tgcctcccgg ccggatggtc
     4381 gcgccgggga aggtcgtcgt gctgctcgtg atgctcacgt gggcgatcga atcggcggcg
     4441 gccgcgttga cctggacggt ccccctcatg ggcgtatgga agatgcagtg gtacgcgtac
     4501 gacccggcgc tcgcgaacgt gtgctgatag acggcgccgg gcccgagcac gcccgagtcc
     4561 agctcggggc ccgcggtgcc cgggccggtg gggttgcttt tcccggagtt gcaggacgtc
     4621 gccgtcgcga ggatcaggac cgggacggcg aggagccgga agacgcgaaa cggagctttc
     4681 atggcggact cctcccttgg cgctgcgggt gtcgtttcga acgtttcgtg ggggtcgcgc
     4741 ggcccgcggt tccggcggcg ctcgaagcct tggatacgct acgcaccgta gcgattcatg
     4801 aaatcgccgt gatcccccct tccgcgctcg aagtcccacg aggagagtga ccgggatcac
     4861 agaagaacgg gcagaccggg ccgatggaaa gcaacttgat aacgctacgt gttgtagcgt
     4921 aatgtcacgg catggcgaca ggggcggatc accctcgggg gcgtccccgc agcgaaaagg
     4981 cccggcaggc catttttgac gccgcctgcg acctgttcga ggagggcggc tatccggcgg
     5041 ccacgatcga ggcgatcgcg gcccgctcgg gggtggccaa gaccacgatc taccggtggt
     5101 ggccgaaccg gcccgcgctg gtcgtggagg tcctgctccg actggcggcg accgcggcgc
     5161 caccgccgga ggccgggttg gatccggttc gggcccttcg gagcgagctc caccgcgtgg
     5221 cgcaggccat ggacgccctt cccggccgcc tcctgctctc gctcttgggc gaggcgcaga
     5281 acgacgccga cgt
//
LOCUS       DASSLZ010000107         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1810766, whole genome shotgun sequence.
ACCESSION   DASSLZ010000107 DASSLZ010000000
VERSION     DASSLZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810766"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..531
                     /locus_tag="VFM00_03960"
     CDS             106..531
                     /locus_tag="VFM00_03960"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET7497432.1"
                     /translation="MDPRRSHGAEPEPRPLESATTGELLGQFFRQTSELLRKEVELAK
                     AEIGSSVKSAVTMTVGFVVAMVFSLLGLGLFCAAAVLGLAASMSAWSAALLVGVVMFV
                     IAGIVAMVARSKVKRLAHPLKETQKTLKEDVQWAKERMA"
     gene            528..779
                     /locus_tag="VFM00_03965"
     CDS             528..779
                     /locus_tag="VFM00_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497433.1"
                     /translation="MKASPEELKREVEEIREGMTPVLDELDHRRHEMMDWRLQLQKHR
                     PAILKGLAAAAAMFTVVTLARRVRHARSARKRRRPPLPV"
     gene            complement(776..1546)
                     /locus_tag="VFM00_03970"
     CDS             complement(776..1546)
                     /locus_tag="VFM00_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7497434.1"
                     /translation="MTLGRDSTRLRIAAVADIHYPHGTDASTVPALVRAVEGADVFLI
                     CGDLTHHGLAEEAAELGKLLAKIPIPILAVLGNHDFHSDQQEEIKRALKDSPVRILDG
                     DAVEIDGVGFAGVKGFASGFGRRVLEPWGEPVVKQFVREAVEESLKLGAALAQLRSER
                     RIALLHYSPIVGTVEGEPPEIQPFLGSSRLEEPLNRMQVSAVFHGHAHHGRTDGTTST
                     GIPVYNVSLPLLRGHPSDPPTVRIVEIERGRPEGEAAG"
     gene            1594..2400
                     /locus_tag="VFM00_03975"
     CDS             1594..2400
                     /locus_tag="VFM00_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497435.1"
                     /translation="MQKPGPKSAAFYTAVLKSLRESDIPFMIGGGYALKRHTGTGRTT
                     RDLDVFVLPQDVEHILSRFAEQGYRTELTFPHWLGKIYKGRAYADVIFSSGNGVALVD
                     EAWFEHAVEDDVLGMRVKLSPAEEMIWSKAFLMERDRYDGADVAHLILARGLDLDWTR
                     LLQRFREYPLVLLAHLVLFLFSFPSARDRIPAWAWEYLLADLERSRHAGEDDPICRGT
                     LLSREQYLEALARGYKDARLPPEGRMRPEDVLHWTRAAQENREAQERKRG"
     gene            2469..>2865
                     /locus_tag="VFM00_03980"
     CDS             2469..>2865
                     /locus_tag="VFM00_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HET7497436.1"
                     /translation="MDSPSGVASDQGCLYSLLIAPRERHEEILDALVRPVADSIQDHP
                     DLHSLFFVRFSEPVWQLRFRVLGRRAWVEGDLRRDVEFRVRDLEASGKIERHEFQRYD
                     RELERYGGEIGMDLAEKLFHADSLACLDLL"
BASE COUNT          451 a          964 c          983 g          467 t
ORIGIN      
        1 gaaactggct tgatgccttc ccgcgattgg agacggagcg gctcgccggg cgatggacgg
       61 gagcccaaac gcacgcaatc cgccgtttcg gcgggaggag gagacatgga tccaaggcgt
      121 tcccatggcg cggagccgga gccgcgcccg ctggagtcgg cgaccacggg cgagctgctg
      181 gggcagttct tccggcagac ctccgagctc ttgcggaagg aagtggagct ggccaaggcg
      241 gagatcggca gcagcgtgaa gtccgcggtc accatgaccg tcggcttcgt ggtggccatg
      301 gtcttcagcc tgctcggcct gggactgttc tgcgccgcgg ccgtgctcgg cctggcggcg
      361 agcatgtcgg cgtggagcgc agcactcctc gtcggcgtcg tcatgttcgt gatcgcgggg
      421 atcgtggcga tggtcgcgcg ctccaaggtg aagcgcctcg cgcatcccct gaaggaaacc
      481 cagaagaccc tgaaggagga cgtgcaatgg gccaaggaac gcatggcgtg aaggcttcgc
      541 cggaagagct gaagcgcgag gtcgaggaga tccgggaggg gatgactccg gtgctggacg
      601 agctggatca tcggcggcac gagatgatgg actggaggct gcagcttcag aagcaccggc
      661 ccgcaattct aaaggggctt gccgcggcgg cggccatgtt cacggtggtg accctggcgc
      721 gtcgcgttcg gcacgcccgc tcggcccgaa agcggcggcg cccaccgctc ccggtctagc
      781 cggccgcctc accctcgggg cggcccctct cgatctccac gatacggacc gtggggggat
      841 cggacggatg gccgcggagc agcggcagcg agacgttgta gacgggaatg cccgtgctgg
      901 tcgtcccgtc ggtgcggccg tgatgggcgt gcccgtggaa cacggccgaa acctgcatcc
      961 ggttgagcgg ctcttccagc cggctgctgc ccaggaaggg ctggatctcc ggcggctccc
     1021 cctcgaccgt gccgacaatc ggggagtagt ggagcagcgc gatgcggcgc tcggatcgaa
     1081 gctgggccag ggcggcgccc agcttgagcg attcctcgac ggcctcgcgc acgaactgct
     1141 tcacgaccgg ctccccccag ggctccagca cgcgccggcc gaatcccgag gcgaatccct
     1201 tcaccccggc gaagcccacg ccgtcgatct cgacggcgtc cccgtcgagg atgcgaaccg
     1261 gggaatcctt gagcgcccgc ttgatctctt cctgctggtc ggagtggaag tcgtggtttc
     1321 cgagcacggc aaggatgggg atcggaatct tggcgagcaa tttccccagc tccgcggctt
     1381 cctcggccag gccgtgatgc gtcaggtcgc cgcagatcag gaaaacgtcg gcgccctcca
     1441 cggccctgac cagggctggc acggtggatg cgtccgtccc atgtggatag tggatgtccg
     1501 cgacggcggc gatgcggagt cgcgtcgaat cccggccgag cgtcattcgg tccctccctc
     1561 ggaaatccca ccccggagcg tcagaacccc atcatgcaga aaccgggccc gaaatccgcc
     1621 gcgttctata ccgcggtgct caagtcgctg cgcgaatccg acattccctt catgatcggg
     1681 ggcggatacg cgctgaagcg ccacaccggc acgggccgca cgacgcgcga cctggacgtc
     1741 ttcgtactgc cgcaagacgt tgaacatatc ctgtcgcgct tcgccgagca gggctaccgc
     1801 accgagctta ccttccccca ctggctgggc aagatttaca aggggagggc ctacgccgac
     1861 gtgatcttca gctcgggcaa cggggtcgcg ctggtggacg aggcctggtt cgagcacgcg
     1921 gtcgaggacg acgtcctggg catgcgcgtc aagctgtccc cggccgagga gatgatctgg
     1981 tcgaaggcat ttctcatgga gcgggaccgt tacgacgggg ccgacgtggc gcacctgatc
     2041 ctggcccgtg ggctggacct cgattggacg cgcctcctcc agcgattccg cgagtatccg
     2101 ctcgtgctgc tcgcgcacct cgttctcttc ctcttctcct ttccgtcggc ccgcgaccgc
     2161 atccccgcct gggcctggga atacctgctg gccgacctcg agcggtcgcg tcacgccggc
     2221 gaggacgatc ccatctgccg cgggaccctc ctctcgcgcg agcagtatct ggaggccctg
     2281 gcgcgcggct acaaggacgc ccggcttcct cccgaggggc ggatgcgccc cgaggacgtc
     2341 ctccactgga cgcgcgcggc ccaggagaat cgcgaagcac aggagcgaaa gcgcggctga
     2401 acgccggccc tcgcggccga gcgtgacggt gggcgtggct ccctcgcgga gcgtacaatt
     2461 cgcctctgat ggactccccc tccggcgtcg catccgatca gggctgcctc tacagcctcc
     2521 tcatcgcccc gcgcgagcgg cacgaggaga tcctggacgc gctggtccgt ccggtcgccg
     2581 actccatcca ggatcatccc gacctccact cgctcttctt cgtccgattc tccgagccgg
     2641 tatggcagct gcgctttcgg gtcctcggac gccgggcgtg ggtggaaggt gacctccgcc
     2701 gggacgtgga gttccgggtg cgcgacctcg aggcgtccgg gaagatcgag cgccatgagt
     2761 tccagcgcta cgatcgcgag ctggagcggt acggcgggga gatcggaatg gacctcgccg
     2821 agaagctctt ccacgccgac tccctcgcct gcctcgatct cctcc
//
LOCUS       DASSLZ010000108         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1810813, whole genome shotgun sequence.
ACCESSION   DASSLZ010000108 DASSLZ010000000
VERSION     DASSLZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810813"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1038
                     /locus_tag="VFM00_03985"
     CDS             277..1038
                     /locus_tag="VFM00_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497437.1"
                     /translation="MSKNRAVRFAVLIAAALGLVAGPRAEAALPAPAQSAVMSYAEEL
                     ARVENAKSRVSLEPLFLKAYAIRHDLVSPAASGAPSPVETLTDGEFVATQGHLRGVAV
                     NREEAVFAVPDAEFFEKLGERKGRTEDRLLLRLYHRTYPRAAWPVYVRQQTGATGCTD
                     FGPGNLTKLYGEWTHYKSLHPDKYRIVVDAEIERITDELTHSDCACGPSGSVQSELRY
                     FEHRHPGSPIAPAVHERLNELLHGKSKIRFNCTSG"
     gene            complement(1076..1825)
                     /gene="pgl"
                     /locus_tag="VFM00_03990"
     CDS             complement(1076..1825)
                     /gene="pgl"
                     /locus_tag="VFM00_03990"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET7497438.1"
                     /translation="MSHLRVVSDPEALAHGAAQEFLRLGAEAIRARGRFDAVLSGGST
                     PRATYERIAETWKEAPGGPLDWARVRLFWGDERHVPPDDPQNNYRMANDAMIEHVPIP
                     PENIYPVPSAIPDAREAASRYEARIMEALGLGPDDVPRFDLILLGMGPDGHVASIFPG
                     TEAVEEEKRLVMGNWVDATKTWRITLTLPVLNHAANVMVLVAGAEKAPALRRVLRGPH
                     TPPLLPAERLHIKDGTLLWLADREAAGAPPD"
     gene            1894..2652
                     /locus_tag="VFM00_03995"
     CDS             1894..2652
                     /locus_tag="VFM00_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7497439.1"
                     /translation="MIEIQGLVRRYGEVEAVSGLSLSIPGGQVFGLLGPNGAGKSTTL
                     RVLATLIRPTEGLVRIGGFDVVARADDVRAIIGYVPEGADLYDALTGFEFLELVADLH
                     RLPRDLAEARRKELLESFALWDDAGRAIGEYSKGMKQKLLITAALQHQPKVLLLDEPL
                     DGLDVAAQEVLKGLIRRQAAGGGVVVYSSHILEVVERVCDRVAIIHRGRVVADDSPAG
                     LLAAHPGETLSEVFLGLTATTAAPVPEERPVETR"
     gene            2649..>2931
                     /locus_tag="VFM00_04000"
     CDS             2649..>2931
                     /locus_tag="VFM00_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497440.1"
                     /translation="MSASDAIIKALGADPAVYRPMARAQARILSRRTRLARGAGRITL
                     KGITPFQLRCFLFAIFSVMMLGWIVGATSPIFGVVLVLTAGTGFLLLDLL"
BASE COUNT          420 a         1034 c         1019 g          458 t
ORIGIN      
        1 atccgtccgc ctccagccac tccgcgtagg gagcgggaag cgccgagggc tccgcggacg
       61 ggcgcggcgg cgccagggtc tgcgtgccgc cttccgacgc gttcttcggc cgtgacgtcg
      121 atgaatgacg cggtaccgcg gggtgcgggc gcttcttcga gggcacgggc agactccctt
      181 gctgcggacg tgacgctccc gactggaacg aaccgatact actccgatcc ggttccttgg
      241 gaagacccct ctctcccggt ggggtatagt ttccgcgtga gcaagaaccg tgcagtgcgt
      301 ttcgcggtgc tgatcgcggc ggccctgggg ctggtggcgg gcccgcgcgc cgaagcggcg
      361 ctccccgcgc cggcccagtc ggcggtgatg agctacgcgg aagagctggc gcgcgtcgag
      421 aacgcgaaga gccgggtttc gctggagccg ctcttcctca aggcgtacgc gatccggcac
      481 gacctggtgt cgccggccgc gtccggcgcg ccctcgccgg tggagacgct caccgacggg
      541 gagttcgtcg cgacgcaggg acacctgcgc ggcgtggccg tgaaccggga ggaggcggtg
      601 ttcgccgtgc ccgacgccga gttcttcgag aagctggggg agcggaaggg gcggaccgag
      661 gaccggctgc ttctacgtct ctatcatcgg acgtacccgc gcgcggcatg gcccgtctac
      721 gtgcgccagc agaccggcgc gaccggctgc accgatttcg gccccggcaa cctgaccaag
      781 ctgtacggcg agtggaccca ctacaagtcg ctccacccgg acaagtaccg catcgtcgtg
      841 gacgccgaga tcgagcggat cacggacgag ctgacccatt ccgactgcgc ctgcgggccc
      901 tcgggatcgg tccagtcgga gctgcgctac ttcgagcacc gccacccggg cagccccatc
      961 gccccggccg tgcacgagcg gctgaacgag ctgctgcacg ggaagtcgaa gatccggttc
     1021 aactgcacgt cggggtagcg gggctgttgt ttgccttctc ggccacgcgc cagggctagt
     1081 ccggcggcgc gcctgccgcc tcccgatccg ccagccaaag cagcgttcca tccttgatat
     1141 ggagccgctc cgccggaagg agcggcggcg tgtgcggacc gcgaagcacg cgccgcagcg
     1201 ccggcgcctt ctcggcgccc gcgaccagca ccatgacatt cgccgcgtga ttcagcacgg
     1261 gcaaggtcag ggtgatccgc caggtcttgg tggcgtccac ccagttcccc atcaccagcc
     1321 gcttctcttc ctcgaccgcc tctgtccccg gaaagatcga ggccacgtgc ccgtccggtc
     1381 ccatgcccag gagaatcaga tcgaagcggg gcacgtcgtc gggccccaaa cccaacgcct
     1441 ccatgatgcg cgcctcgtag cggctcgccg cctcgcgcgc gtcggggatg gccgagggca
     1501 ccggatagat gttctcgggc gggatcggca cgtgctcgat catggcgtcg ttggccatgc
     1561 ggtagttgtt ctgcggatcg tcgggcggca cgtgccgctc gtccccccag aaaaggcgca
     1621 cccgcgccca gtccagcggc ccgccgggcg cttccttcca ggtctcggcg atccgctcgt
     1681 aggtcgcgcg gggcgtactg ccgccggaga ggaccgcgtc gaagcgcccg cgggcgcgga
     1741 tcgcctcggc gcccaggcgc aggaattcct gggccgcgcc atgggccagg gcttccggat
     1801 ccgacacgac gcgcaggtgg ctcatggact cctcgatacc atggacaacg gcttcgccgg
     1861 ggcgcagtct acggcaggga gggtacccat cccatgatcg aaatccaggg gctcgtccgc
     1921 cgctacggcg aggtcgaggc cgtctccggc ctctcgcttt ccatccccgg cggccaggtg
     1981 ttcgggctcc tcggtccgaa cggcgccggc aagtccacga cgctccgcgt cctcgccacg
     2041 ctgatccgcc ccaccgaggg gctggtgcgg atcggcggct tcgacgtggt cgcgcgcgcc
     2101 gacgacgtgc gagcgatcat cggctacgtc cccgaggggg ccgatctcta cgacgcgctg
     2161 accgggttcg agttcctgga gctggtcgcg gacctgcacc gcctgccgcg cgacctcgcg
     2221 gaagcgcggc ggaaagagct gctcgagtcg ttcgcgctct gggacgacgc cgggcgcgcg
     2281 atcggcgagt actcgaaagg gatgaagcag aagctcctga tcacggccgc gctccagcac
     2341 cagcccaagg tcctgctcct ggacgagccg ctggacggcc tggacgtcgc cgcgcaggaa
     2401 gtattgaagg ggctgatccg ccgccaggcc gcgggcggcg gcgtagtcgt ctactcctcg
     2461 catatcctcg aggtggtgga gcgcgtctgc gaccgcgtcg cgatcatcca tcgtggacgg
     2521 gtggtcgcgg acgactcgcc ggcgggcctg ctggcggcgc atcccggcga aacgctctcg
     2581 gaggtgttcc tgggactcac cgccacaacc gctgccccgg ttcccgagga gcggccggta
     2641 gaaacgcggt gagcgcaagc gacgccatca tcaaggcact cggcgccgat cccgccgtct
     2701 accggcccat ggcgcgcgcg caggcgcgca tcctgtcgcg ccggacgcgc ctggctcgcg
     2761 gcgccgggag gatcacgctg aaggggatca ccccctttca gctccgctgc ttcctgttcg
     2821 cgatcttcag cgtgatgatg ctggggtgga tcgtcggcgc gacatcgccc atcttcggcg
     2881 tcgtcctggt cctgaccgcg ggcaccggct ttctgctgct cgatctcctc t
//
LOCUS       DASSLZ010000109        12967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1558962, whole genome shotgun sequence.
ACCESSION   DASSLZ010000109 DASSLZ010000000
VERSION     DASSLZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558962"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2079
                     /locus_tag="VFM00_04005"
     CDS             <1..2079
                     /locus_tag="VFM00_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HET7497441.1"
                     /translation="MHVMSLADPENPVDLASYTKDYVHDSWVRGTRLHAFCISSNTVR
                     IFDLSNPSAPVEQSSWSYLNAKSHSGETSKDGRYLYVCDEVNYSTMKVFDIQDILAHP
                     LVYETTVNPLSIVHNVHVKQDTAFVAWYTEGVHLFDITDPTLPAEFGWYDTYPSYSGG
                     YHGVWEVACGFPSGTFIASDIESGLWIFRTVRNYGVVKVRARDELGQPLTGVDVVHVE
                     SAEDSTQTGMTAGARIALAPGASTLRLSKFGYETAFVSDNVTVGSRDSFVVTMKWVAA
                     TALTGVVRRSSDAAAIQDAAITLENTPLSAVTVSTGVYNIAGVPRGSYVLSAYRPGYA
                     PASRVVVAEPGVARSADFKLLPAAWYDSCDTNRGWSLADADDNAAAGRWERAIPNGTT
                     GQNAPRLPLASGATPGGDRLAFGAAAAGGGVPLRDAQHDEPAEGMLPVGPVAPGVDAT
                     TGTGTGYCFVTGNGPAGGDPAASDVDGGKTTLTTPPIDLTAMTEPMLSWSRWFHMNTP
                     GEPDSFVIQISRDGLSWVTVRSLIQSHPEWHQDVIRVKDYIAPSATVRVRFIAQDQPP
                     ADGVVEAGVDDFMAYDAALQPANVDDTTATAPGAPPLALDSPRPNPASREASVTLRLR
                     AAGSVRVGVFDAAGRHVATIFDGTAPAGPLAVRWDGRDVRGRAAGSGVYWIRAEAAGE
                     KRTTRLVWVR"
     gene            complement(2404..3330)
                     /locus_tag="VFM00_04010"
     CDS             complement(2404..3330)
                     /locus_tag="VFM00_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET7497442.1"
                     /translation="MKRTGQVPFMKLQVGILSLIALALILWATFQSGSFKFGKEEEIT
                     VHFANVGGLETGSPVRLNGVPVGTVRKIELGDSTNVVKVVMGVKDGTRARLHQGATAR
                     ITTVGFLSELYIALDAGNEALPMIASDEEIQPAIITDPQVLLTQAKGMADSVEVILSN
                     LNAATRGFTNGRGTLGRLAQDEKLYDNLVAMSRNASDLAARMNQTQSRVADRLMALAA
                     SFDSLSWRMQHGEGTLAQLMNNGELHRNLASSTARMDSVMALMEGGKGSMGKLMADST
                     LYEDTKALMGSMKRLMAEIEKDPKKYFKFSLF"
     gene            complement(3344..4144)
                     /locus_tag="VFM00_04015"
     CDS             complement(3344..4144)
                     /locus_tag="VFM00_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7497443.1"
                     /translation="MISFEHVDYSIDGRPILRDITFNVPLGGTTVILGPSGSGKSTTL
                     RLLLGLARPDAGVIRIDNEDITKMSEKRLRQIRQRIGMVFQQGALFDSMTVGENVAFS
                     LIEHDNVPDEELEGTVRAHLELVGLDPDLIDRMPDQLSGGMQRRVAIARALAARNPQI
                     LLYDEPTTGLDPQSAERITDLIVELRDRMKKTSVMVTHDIADAFKVGNTMAVLNEGRL
                     VFEGTPADLARTNEPFISEFLAPFRKAVLAATQRVVTVPGTPRPTAPQ"
     gene            complement(4151..4930)
                     /locus_tag="VFM00_04020"
     CDS             complement(4151..4930)
                     /locus_tag="VFM00_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7497444.1"
                     /translation="MGLIAAIVERLRRILEVVQDFVLFAWDAIRATFTPPYYGREFLS
                     QLHFALVGSLLIVVVSAAVAGQALAIQLVRELANTGAKSQLGHFMVISVVRALGPVLT
                     GMVVASRMSAGITAEIGAMRSSDQIDALVAFGSDPMKRLVVPRVAALLIALPVLVIIG
                     DALAVVGGGFVGLQYHLPVESYYSGVLKYLTPKNVLVGMIKPFVFALMIATVACWRGF
                     RSAGGAKGVGVSTTESVVISSVGILVADGVCTRLVFRILGW"
     gene            complement(4959..5612)
                     /locus_tag="VFM00_04025"
     CDS             complement(4959..5612)
                     /locus_tag="VFM00_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET7497445.1"
                     /translation="MAKANYGDLPINHSEEPIRLFKSDFLESFTHIHPAVVLVLYVPV
                     VLFFLARAFREQGGGAIGPVFGMYTVGLLVWSFSEYMLHRYLFHWEPQLPFLKRAWYL
                     VHGVHHEQPQCKTRLVMPPVLSIPLAILFFLFFKTLIGTIFGLPLWVAPLFAGFVTGY
                     IAYDMMHYAEHHLSMTWGFLKFVKRYHLLHHYKTPEHRFGVSSPLWDYIFGTKPKGR"
     gene            5736..6431
                     /locus_tag="VFM00_04030"
     CDS             5736..6431
                     /locus_tag="VFM00_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HET7497446.1"
                     /translation="MPGTDPKPLRVNEIFLSVQGEGIHVGERTVFLRLYGCPLRCVWC
                     DQPEALATSGIGRFETMLPNAIRDRILAYPAVRRLCLTGGEPLIAPSESLAWLLGELR
                     AAGWWISVETSGARVVEPLLPLIDFWTISPKGESARTFDGDPVEAQLPTLRQLVRELP
                     EVRRQLKFVIQSEGDVRDAVRILDALEYRGAVVLQPEHGGGDGRAAFEWWPWDRYPEA
                     RIIPQTHKAVGLR"
     gene            6428..6793
                     /gene="queD"
                     /locus_tag="VFM00_04035"
     CDS             6428..6793
                     /gene="queD"
                     /locus_tag="VFM00_04035"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HET7497447.1"
                     /translation="MNVRLTRDFTFEAAHLLPNAPEGHKCRRLHGHSFRVEVTVEGAV
                     DPRTGWFLDYGVIKDAVEPLRRQLDHYYLNEIPGLENATSEILAGWLWDRLKPALPSL
                     ARVTVHETCEARCEYEGRE"
     gene            6971..7648
                     /locus_tag="VFM00_04040"
     CDS             6971..7648
                     /locus_tag="VFM00_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7497448.1"
                     /translation="MWVITLIVGVWFLAAEGILLWFTFRYRKKEGVRAAYVPGKGFRQ
                     TGWILIPCVAILLCDLAIDAFGERVWDTIKQKVPPAQLQIGVEGRQWAWTFTHPGKDG
                     ILGSPDDIVIPNEIHVPVGVNVAFTVSAADCLHSFWVPELRLKQDAVPGRHIQGWFRA
                     TRPGSYGVLCAELCGVAHGLMRGTLYVDTEADYERWLASRTGVQAVASQPAPQTAPQT
                     AKTGGKS"
     gene            7645..9390
                     /locus_tag="VFM00_04045"
     CDS             7645..9390
                     /locus_tag="VFM00_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7497449.1"
                     /translation="MSEANILDPPLDPPQAAPPTAHAGAHDDAPHGFWSTYFFSTDHK
                     HIAKQYLLTGLCMAVIGGLLAHVMRMHLAFPDRAVPGFGEVPAGTYNALITMHGTIMI
                     FFVAMPVLLGGFGNFLIPLMIGARDMAFPRLNMMSYWTFLVSCVILIGSFFVPGGASP
                     AGWTAYPPLSANVAYSGAAWGVPLWIAAVALEFVAFLMGGINFITTAINLRAPGMKMF
                     DLPMMVWMQLVASVIFLFSVGPLVAGAVLLLLDRVAGTGFYNPGAGGDPLLYQHLFWF
                     FGHPEVYVILLPGLGIVAEILPVFARKPLFGYRMVVYATLATGVLSFIVWAHHQFVSG
                     IDPRLASAFSITTILISVPVAITMFAFVATLWKGAIELKAAMLFAIGMVGVFFWGGVT
                     GIINGSAAADIYVHDTYFVVAHFHYALIPPVFFSLFAGIYYWYPKMFGRRLNETLGKI
                     HFWGTFLFVNVTFMPMFGLGLRGYPRRTYDPRVFAHLGDLHPLQLAATLGTIGLLLSQ
                     IPFVINFFGSLKWGAVAGKNPWRANTLEWSVPSPPGHGNFDGTPVVYRGPYEYSLPGG
                     DADWLPQDLAPGRTP"
     gene            9387..9983
                     /locus_tag="VFM00_04050"
     CDS             9387..9983
                     /locus_tag="VFM00_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET7497450.1"
                     /translation="MSRAAAHAAATRRDDVIRSTGISTGRLAIWWFLGSEIVIFGGLI
                     VCYVLFRLRHPEWGALARHTVTPAGAFNTLVLLTSSLFMVLAHAAVEHGDLKRASRHM
                     LGTIALGGVFLGVKAFEYGHEMSMGFTPVSGLFWSFYYALTGLHALHVTGGLFAILAV
                     RQMVARRSDTHRVELVGIYWHFVDVVWIFLFPLLYLSN"
     gene            10000..10296
                     /locus_tag="VFM00_04055"
     CDS             10000..10296
                     /locus_tag="VFM00_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HET7497451.1"
                     /translation="MTHTIPAPERRHPNYVAIWGVLMGALILSLLLAYLRHAQLAALL
                     IWLVAIVKASLVISFYMHLKFEPAHVVAIVIAGVLALAILFLGLFPDIVRVYGG"
     gene            10304..10906
                     /locus_tag="VFM00_04060"
     CDS             10304..10906
                     /locus_tag="VFM00_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET7497452.1"
                     /translation="MDRAATRTLTPIDRAVETGVIGMTLFLFTEAMFFAGLVSAHVVL
                     RAGQPSWPPAGQPRLPMGLTLANTVVLLLSGAAVVRASASFPERLDAAARAIGAGALL
                     GVVFLTVQGLEWARLTAFGLTTTSGIYGATFYALVVAHAIHVLAALIVLLVAARKMAR
                     ASREGRPDDARVAFTLSRMFWLFVVGVWPVLYAVVYEPWR"
     gene            10897..11112
                     /locus_tag="VFM00_04065"
     CDS             10897..11112
                     /locus_tag="VFM00_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497453.1"
                     /translation="MALMQGVAALVDAALAVAHALFFRLLPAACAFCASSSQTNKAAY
                     IGTTVLLLGLPVAMLSALIVWLIRRAR"
     gene            complement(11093..12469)
                     /gene="hemG"
                     /locus_tag="VFM00_04070"
     CDS             complement(11093..12469)
                     /gene="hemG"
                     /locus_tag="VFM00_04070"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HET7497454.1"
                     /translation="MRVVIIGGGIAGLSAAWAIRAEAARRAESLELTVLEAGSRAGGR
                     IRSTREDGYLVEWAANAVQGTENAAWRLAESVGLADQRVLGRPDAARRYIWRGGRLHR
                     LPLNPAAFFGFTGLSPAARLRVAMEPFFANRVKREESVHDYAVRHIGEEAASVLIGAV
                     VRGVFAGNSKRLSVDAAFPVMREMERSHRSLVVAMIARSKKGGAGRAAGPGGRALWSF
                     TRGLESLTDALAARLGNAVRIATPALALERTESGYVVRTASGDRLAADSVVLAISARA
                     AAALLRPLDAELAKLLGTIEPAGVAVVGLAFRTDRFRTPPDGYGYLVVPGEDLPILGA
                     LFESNLFPGRAPEGQTMVRVIMGGADRPDLLTRSDADLAGLAMGALDKTHGLASGPER
                     TWVIRQDASIPQYAVGHMALLADLDRRLAANPRLYLAGNGYRGVSVASLTEDAERIAA
                     RVVSAPGG"
     gene            12619..>12967
                     /locus_tag="VFM00_04075"
     CDS             12619..>12967
                     /locus_tag="VFM00_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497455.1"
                     /translation="MLSAQLKAAVAGAPAPAPGKCLEIQYEGVLELEGHFRKPGEKQP
                     YRSRQRFLANANGAVRLDWTTWIQGDSMGAPESWLLLPAGVGGTILHRSAPGSPWREV
                     GVDQREQALAQVTA"
BASE COUNT         1938 a         4484 c         4377 g         2168 t
ORIGIN      
        1 atgcacgtca tgtcgctcgc cgatcccgag aatcccgtcg acctcgcctc gtacaccaag
       61 gactacgtgc acgattcgtg ggtgcgcggc acgcggctcc acgccttctg catctcgagc
      121 aacacggtcc ggatcttcga cctgagcaac ccctcggcgc cggtcgagca atcctcctgg
      181 agctacctga acgccaagtc gcacagcggc gagacctcca aggacggccg gtatctctac
      241 gtctgcgacg aggtgaacta ctccacgatg aaggtcttcg acatccagga catcctggcg
      301 catccgctcg tgtacgagac caccgtgaat ccgctctcga tcgtgcacaa cgtgcacgtg
      361 aagcaggaca ccgcgttcgt cgcctggtac acggaggggg tgcatctgtt cgacatcacc
      421 gacccgacgc tgccggcgga gttcggctgg tacgacacct atccctcgta ttcgggcggc
      481 taccacggcg tctgggaagt ggcgtgcgga tttccgtcgg ggaccttcat cgcgagcgac
      541 atcgagagcg gcctctggat cttccgcacc gtccgaaact acggcgtggt caaggtgcgc
      601 gcccgggacg aactgggaca gcccctgacc ggcgtggacg tggtccatgt ggagtccgcc
      661 gaggattcca cgcagaccgg catgaccgcc ggggcgcgga tcgcgctcgc gccgggtgcc
      721 tccaccctgc gactgtcgaa gttcggctac gagacggcgt tcgtcagcga caacgtgacg
      781 gtcggaagcc gggacagctt cgtggtcacg atgaagtggg tggcggccac cgcgctcacg
      841 ggcgtggtgc ggcggtcctc ggacgcggcg gcgattcagg acgcggcgat cacgctcgag
      901 aacacgccgc tctcggcggt cacggtctcg acgggggtct acaacatcgc gggcgtgccc
      961 cgggggagct acgtcctgag cgcctatcgc ccgggctacg ccccggcctc gcgcgtggtc
     1021 gtcgccgagc ccggcgtggc ccggagcgcg gatttcaagc tgctcccggc cgcctggtac
     1081 gactcctgtg acacgaaccg gggatggagc cttgccgacg cggacgacaa tgccgcggcc
     1141 ggacggtggg agcgcgcgat tccgaacgga acgacggggc agaacgcgcc ccgacttccg
     1201 ctcgcgtcgg gcgccacgcc tgggggggac cgtctggcgt ttggggcagc ggccgcgggc
     1261 ggcggcgtcc cgctccgcga cgcgcagcac gacgagccgg cggaggggat gcttccggtc
     1321 ggtccggtcg cgccgggcgt cgacgcgacg accggaacgg ggaccggcta ctgcttcgtc
     1381 accgggaacg gtccggccgg cggcgatccg gcggcgtcgg acgtggacgg cggcaagacc
     1441 acgctgacca cgccgccgat cgacctgacc gccatgacgg agccgatgct ctcctggtcg
     1501 cggtggttcc acatgaacac gcccggcgag cccgactcgt tcgtgatcca gatcagccgc
     1561 gacggtctga gctgggtcac ggtccgctcg ctgattcaga gccaccccga atggcatcag
     1621 gacgtgatcc gcgtgaagga ctacatcgcg cccagcgcca ccgtgcgcgt gcgcttcatc
     1681 gcgcaggacc agccgcccgc cgacggcgtc gtggaggcgg gcgtggacga cttcatggcc
     1741 tacgacgccg cgctccagcc cgccaacgtc gacgacacca ccgcgaccgc tcccggcgcg
     1801 ccgccgctgg cgctggactc gccgagaccg aatcccgcct cgcgcgaggc gtcggtgacg
     1861 ctgcgcttac gggcggcggg ttcggtgcgg gtcggcgtgt tcgacgcggc cggccgccat
     1921 gtcgcgacga tcttcgatgg aacggctccg gcgggcccgc tggcggtgcg ctgggacggc
     1981 agggacgtac ggggccgcgc ggcgggcagc ggcgtctact ggatccgcgc cgaggcggcg
     2041 ggggagaagc ggacgacgag actggtgtgg gtgcggtgag gctcagctac gggacgagga
     2101 agcctggaga gtgactggcg tcgggtgcga cacggacttg agagagacgt gtagcgtggc
     2161 acaaggaacg tcttccagaa catgtcacac tgcggccgct ccactgcagc cgctccatga
     2221 tgaactgacc ctccgaagca ttaatatata agttaatatg gcctatcgtt caacatactc
     2281 cagagtatgc acgatgtgcc gcttcggcac agatcgccgc aatcagtgga tgtataccgc
     2341 acctggattc tgggtgagcc gccgccgatt gcggccggga gcgcagaacc cggcgggccc
     2401 tctctagaac aaactgaact tgaagtactt cttggggtcc ttctcgatct ccgccatcag
     2461 gcgcttcatc gagcccatca gcgccttcgt atcctcgtaa agcgtcgagt cggccatcag
     2521 cttgcccatc gaacctttgc ccccctccat cagcgccatc accgaatcca tccgcgcggt
     2581 gctcgaggcg agattccggt gcagctcgcc gttgttcatc agctgcgcca gcgtcccctc
     2641 gccgtgctgc atccgccagg agaggctgtc gaaggaggcg gccagagcca tcaggcggtc
     2701 ggccacgcgg gactgggtct ggttcatgcg cgcggcgaga tcgctcgcgt tccggctcat
     2761 cgcgaccagg ttgtcgtaga gcttctcgtc ctgggccagc cgccccaggg tgccgcgccc
     2821 gttcgtgaag ccgcgcgtcg cggcgttcag gttggagaga atcacctcca cgctgtccgc
     2881 catccccttg gcctgggtga gcaggacctg ggggtcggtg atgatggcgg gctggatctc
     2941 ctcgtcgctc gcgatcatcg ggagcgcctc gtttcccgcg tcgagcgcga tgtacagctc
     3001 ggagaggaag cccacggtcg tgatgcgcgc ggtcgcgcct tgatggaggc gcgcgcgggt
     3061 gccgtccttc acgcccatca ccaccttcac gacgttggtg ctgtcgccca gctcgatctt
     3121 gcgcacggtg ccgaccggga cgccgttcag gcggacggga gagccggtct ccagcccgcc
     3181 gacgttggcg aagtggaccg taatctcctc ctccttcccg aacttgaagc tgcccgactg
     3241 gaacgtggcc cagaggatca gggcgagcgc gatcagcgac aggatcccga cctggagctt
     3301 catgaagggc acctgcccgg ttcgcttcac gccattcctc ccgtcactga ggcgccgtgg
     3361 gccgcggggt ccccggaacc gtgacgacgc gctgcgtcgc ggccaggacc gccttgcgga
     3421 acggggccag gaattcgctg atgaacggct cgttggtgcg ggccagatcg gccggcgtgc
     3481 cctcgaacac gagccgtccc tcgttcagca ccgccatggt gttccccacc ttgaacgcat
     3541 cggcgatgtc gtgggtcacc atgaccgacg tcttcttcat gcggtcgcgc agctccacga
     3601 tgagatcggt gatccgctcc gcgctctgag ggtccaggcc ggtcgtcggc tcgtcgtaga
     3661 ggaggatctg cggattgcgc gccgcgagcg cgcgcgcgat cgccacgcgc cgctgcatcc
     3721 cgcccgagag ctgatccggc atccggtcga tcaggtccgg atccagcccc accagctcca
     3781 ggtgcgcgcg aacggtccct tccagctcct cgtcgggcac gttgtcgtgc tcgatcaggg
     3841 agaacgccac gttctcgccg accgtcatcg agtcgaagag cgccccttgc tggaacacca
     3901 tcccgatgcg ctggcggatc tgccggaggc gtttctcgct catcttcgtg atgtcctcgt
     3961 tgtcgatccg gatcacgccc gcgtcgggcc ttgccaggcc cagcagcaga cgaagcgtcg
     4021 tgctcttccc cgagccggac gggcccagga tgaccgtcgt tccgcccagg ggaacgttga
     4081 aggtgatgtc ccggaggatc gggcggccgt cgatcgagta gtcgacatgt tcgaacgaga
     4141 tcataggcgc tcaccaaccc aggatgcgaa acacgagccg cgtgcacacg ccgtcagcca
     4201 ccaggattcc cacggaggag atgacgaccg actccgtggt cgagacaccc acccccttcg
     4261 cccctcccgc ggaccggaat ccgcgccagc aggccaccgt ggcgatcatc agcgcgaaga
     4321 cgaacggctt gatcatgccg accagcacgt tcttgggcgt caggtacttg agcacgcctg
     4381 aatagtatga ctcgaccggc agatggtact gcaaccccac gaacccgccg ccgaccacgg
     4441 cgagcgcgtc gccgatgatc acgagcacgg ggagcgcgat caggagcgcc gccacgcgcg
     4501 gcacgacgag ccgcttcatc gggtcggagc cgaacgccac cagggcgtcg atctggtcgc
     4561 tggagcgcat cgctccgatc tccgccgtga tccccgccga catgcgcgag gccaccacca
     4621 ttcccgtcag gacggggccg agagcccgga cgaccgagat caccatgaag tgcccgagct
     4681 gcgacttggc gcccgtgttc gccagctcgc gcaccagctg gatcgcgagc gcctgtcccg
     4741 ccaccgcggc cgatacgacc acgatcagga gcgaccccac cagggcgaag tggagctggc
     4801 tcaggaactc gcgcccgtag tacggaggcg tgaaggtcgc ccggatcgcg tcccaggcga
     4861 agagcacgaa atcctgcacg acctccagga tgcggcgcag ccgctcgacg atcgccgcga
     4921 tcaggcccac ggcgtcctgc cggctcgccg cggaggcgtc agcgtccctt cggtttggtg
     4981 ccgaagatgt agtcccagag aggggagctg accccgaacc ggtgctcggg ggtcttgtag
     5041 tggtggagca ggtggtagcg cttcacgaac ttgaggaagc cccacgtcat cgagaggtgg
     5101 tgctccgcgt agtgcatcat gtcgtaggcg atgtagccgg tcacgaatcc ggcgaacagc
     5161 ggcgccaccc agagcgggag cccgaagatc gtgccgatca gggtcttgaa gaagaggaag
     5221 aagaggatgg cgagcgggat gctcaggacc ggcggcatga ccaggcgcgt cttgcactgc
     5281 ggctgctcgt ggtggacgcc gtgcaccagg taccacgccc gcttcaggaa cggaagctgc
     5341 ggctcccagt ggaacaggta gcgatgcagc atgtattcgg agaagctcca gaccaggagc
     5401 cccaccgtgt acatgccgaa caccggcccg atggcgccgc ccccctgctc gcggaaggca
     5461 cgcgccagga agaagagcac caccggcacg tagagcacga gcaccacggc cgggtggatg
     5521 tgggtgaagg actcgaggaa gtccgacttg aagagccgga tcggctcctc gctgtggttg
     5581 atcggcaggt cgccgtagtt cgccttggcc atggtcgcgc aacctccgtt cctgttgcct
     5641 gccgggcagt tccgtcggcc cggggtcgat tgcccgggat tgtacgaacg cccacggaac
     5701 aggtcaatgc ccggggaaac cggtagaatc gtccgatgcc cggaactgat ccgaagccgc
     5761 ttcgcgtgaa cgagatcttc ctctccgtcc agggggaggg gatccacgtc ggcgagcgga
     5821 ccgttttcct tagactctac ggctgcccgc tccgatgcgt ctggtgcgac cagcccgagg
     5881 cgctcgccac cagcggcatc gggcgtttcg aaacgatgtt gccgaatgcc attcgggatc
     5941 gaatcctggc gtatcctgcc gtccgccggc tgtgcctgac cggcggggag ccgctgatcg
     6001 ctccctcgga atcgctggcg tggcttctcg gggagctgcg cgcggccggc tggtggatct
     6061 cggtggagac gagcggggcg cgcgtggtcg agccgctcct gccgctgatc gatttctgga
     6121 cgatctcgcc caagggggag agcgcccgga cgttcgacgg cgatcctgtg gaggcccagc
     6181 tcccgacgct gcgtcagctg gtgcgcgagc tgcccgaggt ccggcgccag ctcaagttcg
     6241 tgatccagtc ggagggtgac gtgagggacg cggtccggat cctggacgcc ctggagtacc
     6301 ggggcgccgt ggtgctccag cccgagcatg gcggcgggga cggccgtgcc gcgttcgagt
     6361 ggtggccctg ggatcgctac cccgaggcgc ggatcattcc gcagacgcac aaggcggtgg
     6421 ggctgcgatg aacgtccggc tgacccgcga cttcacgttc gaggccgcgc atctcctccc
     6481 gaacgcgccc gagggacaca agtgccgccg cctgcacggg cattccttcc gcgtcgaggt
     6541 gaccgtggaa ggagcggtcg atccgcgcac cggatggttc ctggactacg gcgtgatcaa
     6601 ggacgccgtc gagccgctcc gccgccagct cgaccactac tacctgaacg agatccccgg
     6661 tctcgagaac gccacgtcgg agattctcgc gggctggctc tgggatcggt tgaagcccgc
     6721 gctcccgtcg ctcgcgcgtg tcacggtcca cgagacgtgc gaagcgcgct gcgagtacga
     6781 gggccgcgag tagccgatag gtatacctaa gtaccatccg caggtatccc tcggctccat
     6841 agcgaccccg cgcagcatcc cgcattctcc cccgagttaa tccccattaa catttgccac
     6901 cgagggctcg ccacgcatgc acctaatgcc tgttgccgca tccagttacg cgggcgatat
     6961 cgatcgcgtg atgtgggtca tcaccctgat cgtgggggtc tggttcctcg ccgccgaggg
     7021 gatcctcctc tggttcacgt tccgctatcg aaagaaggag ggcgttcgcg cggcctacgt
     7081 tccgggaaag gggtttcgcc agaccggctg gatcctgatc ccctgcgtcg cgatcctcct
     7141 ctgcgatctc gccatcgacg ccttcggcga gcgcgtctgg gacacgatca agcagaaggt
     7201 cccgccggcg cagctccaga tcggcgtcga gggacggcaa tgggcgtgga cgttcacgca
     7261 tccggggaag gacggcatcc tcggctcacc tgacgacatc gtcatcccca acgagatcca
     7321 cgtgccggtc ggggtcaacg tcgcgttcac ggtcagcgcg gccgactgcc tccactcctt
     7381 ctgggttccc gagcttcgcc tgaagcagga cgcggtcccc gggcgccaca ttcagggatg
     7441 gttccgcgcg acgcggcccg gcagctacgg cgtgctctgc gcggagctgt gcggcgtggc
     7501 acacgggctc atgcgcggga cgctctacgt ggacaccgag gcggactacg agcgatggct
     7561 cgcctcgcgg acgggcgtcc aggcggtcgc ttcccagcct gctcctcaga cggctccgca
     7621 gacggcgaag acgggaggga aatcgtgagc gaagccaaca tcctcgaccc gcctctcgat
     7681 ccgccccagg cggcgcctcc gacggcccat gccggggccc acgacgacgc cccgcacggc
     7741 ttctggagca cctacttctt ctcgaccgac cacaagcaca tcgccaagca gtacctgctc
     7801 accggtcttt gcatggcggt gatcggtggg ctgctcgcgc acgtgatgcg gatgcacctc
     7861 gcgtttcccg atcgcgcggt gcccggattc ggcgaggtcc cggcgggcac ctacaacgcg
     7921 ctgatcacga tgcacggcac gatcatgatc tttttcgtgg cgatgccggt gctcttgggc
     7981 ggcttcggga atttcctgat cccgctcatg atcggcgcgc gcgatatggc gtttccgcgg
     8041 ctgaacatga tgtcctactg gaccttcctc gtgagctgcg tgattctcat cggctccttc
     8101 ttcgtgccgg gaggcgcctc gcccgcggga tggaccgcgt atccgccgct ctcggccaac
     8161 gtggcctatt ccggcgccgc ctggggcgtg ccactctgga tcgccgcggt cgcgctcgag
     8221 ttcgtcgcct tcctcatggg cgggatcaat ttcatcacca ccgcgatcaa cctccgcgcg
     8281 ccggggatga agatgttcga cctgccgatg atggtctgga tgcagctcgt cgccagcgtg
     8341 atctttctct tctcggtggg gccgctcgtc gcgggcgcgg tgctcctcct gctcgaccgc
     8401 gtggcgggca ccggcttcta caatcccggc gccggcggcg atccgctcct ctaccagcac
     8461 ctcttctggt tcttcggcca tcccgaggtg tacgtcatcc tcctcccggg cctcggcatc
     8521 gtcgcggaga tcctccccgt cttcgcgcgg aagccgctct tcggctaccg gatggtggtc
     8581 tacgccacgc tggccacggg cgtcctgtcg ttcatcgtct gggcgcacca ccagttcgtg
     8641 agcggcatcg acccgcggct ggcgagcgcg ttcagcatca ccacgatcct gatctcggtc
     8701 cccgtcgcga tcacgatgtt cgccttcgtc gcgacgctct ggaagggcgc gatcgagctc
     8761 aaggcggcga tgctcttcgc gatcgggatg gtcggcgtct tcttctgggg cggcgtgacg
     8821 gggatcatca acggctccgc ggccgccgac atctacgtgc acgataccta cttcgtggtg
     8881 gcgcacttcc actacgcgct gatcccgccg gtcttcttct cgctcttcgc cgggatctac
     8941 tactggtatc ccaagatgtt cggccggagg ctgaacgaga cgctggggaa gatccacttc
     9001 tggggcacct tcctcttcgt gaacgtcacg ttcatgccga tgttcggcct gggactgcgc
     9061 ggctacccgc ggcggaccta tgatccgcgg gtcttcgcgc acctcggcga tctgcacccg
     9121 ctccagctgg cggcgaccct gggtaccatc gggctcctcc tctcgcagat ccccttcgtg
     9181 atcaacttct tcggcagcct caagtggggc gcggtcgcgg ggaaaaaccc ctggcgcgcg
     9241 aacacgctcg agtggtcggt gccgtcgcct ccggggcacg gcaacttcga tgggacgccg
     9301 gtcgtctatc gcggccccta cgagtacagc cttccgggag gcgacgccga ctggctgccg
     9361 caagacctgg cgccggggag gacgccgtga gccgggccgc cgcacacgcc gccgcgacgc
     9421 ggcgcgacga cgtcatccgg tcgaccggaa tctccaccgg gcggcttgcg atctggtggt
     9481 tcctgggctc ggagatcgtg attttcggcg ggctgatcgt ctgctacgtg ctcttccgcc
     9541 tgcgccaccc ggagtggggc gccctggcgc gccacaccgt cacgccggcg ggcgcgttca
     9601 acacgctcgt gctgctgacg tccagcctgt tcatggtcct ggcgcacgcg gcggtggagc
     9661 acggcgatct gaagcgggcc tcgcgccaca tgctcgggac gatcgccctg ggaggggtgt
     9721 tcctcggcgt gaaggccttc gagtacggcc acgagatgtc gatgggcttc acgcccgtga
     9781 gcggcctctt ctggtccttc tactacgcgc tcacgggact gcatgcgctg cacgtgacgg
     9841 gcgggctctt cgcgatcctg gcggtgcggc agatggtggc gcgccggagc gacacccacc
     9901 gcgtggagct ggtcggcatc tactggcact tcgtggacgt ggtctggatc ttcctgttcc
     9961 cgcttctcta cctgtccaac tgatgggggc gctcgcgcga tgacccatac gattccggcg
    10021 ccggagcggc gccaccccaa ctacgtggcg atctggggcg tgctgatggg tgctctgatc
    10081 ctgtcgctcc tcctggccta tctccggcac gcccagctcg cggcgctcct catctggctc
    10141 gtcgcgatcg tcaaggcgtc gctggtcatc tcgttctaca tgcacctcaa gttcgagccc
    10201 gcgcacgtcg tggcgatcgt gatcgccggc gtcctcgcgc ttgccatcct cttcctcggc
    10261 ctcttcccgg acatcgtccg tgtctacggc ggctagcgct tccgtggacc gcgccgccac
    10321 gcgcacgctg accccgatcg accgcgccgt cgagaccggg gtcatcggca tgaccctgtt
    10381 cctcttcacc gaggcgatgt tcttcgccgg gctggtcagc gcgcacgtgg tgctccgcgc
    10441 ggggcagcca tcctggccgc cggccgggca gccgcggctt ccgatgggac tcacgctcgc
    10501 gaacacggtc gtcctcctgc tgagcggggc cgcggtggtc cgagcgagcg cctccttccc
    10561 ggagcgattg gacgcggcgg cgcgcgccat cggcgccggc gctttgctgg gcgtggtctt
    10621 cctgaccgtg caggggctgg agtgggcgcg actcaccgcc ttcggcctga cgacgacgtc
    10681 ggggatctac ggggcgacct tctatgcgct ggtcgtggcg cacgcgatcc acgtgctcgc
    10741 ggcgctgatc gtgctgctgg tggcggctcg gaagatggcg cgggcgtcgc gcgagggccg
    10801 cccggacgat gcccgcgtgg cgttcacgct ctcgcgcatg ttctggctct tcgtggtggg
    10861 ggtgtggccg gtgctctacg cggtggtgta cgagccgtgg cgctgatgca gggcgtggcg
    10921 gcgcttgtcg acgcggcgct ggccgtggcg catgcgctgt tcttccggct gttgcccgcg
    10981 gcctgcgcct tctgcgcctc gtcgtcccag accaacaagg ccgcctacat cggcacgacg
    11041 gtgctcctcc tgggcctgcc ggtggcgatg ctctccgcgc tgatcgtctg gctcatccgc
    11101 cgggcgcgct gacgacgcgc gccgcgatcc gctccgcgtc ctccgtcagc gacgccaccg
    11161 acacgccgcg atacccgttc cccgccagat agagccgggg attcgccgcc agacggcggt
    11221 ccaggtcggc caggagcgcc atgtgcccga ccgcgtactg gggaatcgag gcgtcctgcc
    11281 ggatcaccca cgtccgctcg gggccgctcg ccagaccgtg cgtcttgtcc agcgcgccca
    11341 tcgccagccc cgcgaggtcg gcgtcgctcc gcgtgagcag atcgggccgg tcggccccgc
    11401 ccatgatgac gcgcaccatc gtctgcccct cgggcgcgcg tccggggaag aggttcgatt
    11461 cgaacagcgc gcccaggatc ggcaggtcct cgccgggcac gaccaggtag ccgtagccgt
    11521 ccgggggtgt gcggaagcgg tcggtccgga aggccagccc caccacggcc actcctgcgg
    11581 gctcgatcgt gccgagaagc ttggcgagct cggcgtcgag cggacgcagg agcgccgccg
    11641 ccgcccgcgc ggagatcgcg agcacgactg aatcggcggc gagacggtcg ccgctcgcgg
    11701 tgcgcacgac gtagccggat tcggtgcgct ccagcgcgag ggccggcgtg gcgatccgaa
    11761 ccgcgttccc caatcgggcc gcgagggcgt cggtcagcga ttcgagcccg cgcgtgaagc
    11821 tccagagggc gcgccctccc ggacccgcgg cgcggccggc gccgcccttc ttggaccgcg
    11881 cgatcatcgc cacgaccagg gagcggtggc tccgctccat ctcacgcatg acggggaagg
    11941 ccgcgtccac tgagagccgc ttcgagttgc cggcgaagac cccgcgcacc acggcgccga
    12001 tcaggacgct ggccgcctcc tcgccgatgt ggcgtaccgc atagtcgtgg acgctttctt
    12061 ctcttttgac gcgattggcg aagaagggct ccatggcgac ccggagccgc gcggcggggg
    12121 aaaggccggt gaagccgaaa aacgcggccg ggttcagggg gagccgatgg aggcgtccgc
    12181 cgcgccagat gtagcgccgc gccgcgtcgg ggcgccccag cacccgctgg tcggccaggc
    12241 ccaccgattc ggccagccgc cacgcggcgt tctccgttcc ctggaccgcg ttcgccgccc
    12301 attcgaccag gtagccgtcc tcgcgtgtcg agcggatccg cccgcccgcg cgcgatcccg
    12361 cctccagcac ggtcagctcg agcgactcgg cgcgccgcgc cgcctcggcc cggatcgccc
    12421 acgccgcgga gagacccgcg atgcctccac cgatgatgac gacgcgcatg cgcggatact
    12481 agcgttccgg ggcggaaggc cctagtgttc tgaggcatgc ctttcgcaac cccctggacc
    12541 ctccgccgcg ccgcggcgct cgccctgtgt gcgacttttc tcgcgccctc gccgcccggg
    12601 aggccgacgc cggccgcgat gctctccgcg cagctcaagg ccgcggtcgc cggcgcgccc
    12661 gcgcccgcgc cgggcaagtg tctggagatc cagtacgagg gagtcctcga gctggaaggg
    12721 cacttccgca aacctggcga gaagcagccc tatcgctcgc ggcagcgctt cctggccaac
    12781 gccaatgggg cggtccgtct ggactggacc acatggatcc agggcgattc gatgggcgcg
    12841 ccggagagct ggctcctcct gcctgccggc gtcggcggaa cgatcctcca tcgctcggcg
    12901 ccgggcagcc cgtggcgcga ggtcggggtg gatcagcgcg agcaggctct ggcccaggtc
    12961 acggcgg
//
LOCUS       DASSLZ010000110         5106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_418610, whole genome shotgun sequence.
ACCESSION   DASSLZ010000110 DASSLZ010000000
VERSION     DASSLZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418610"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFM00_04080"
     CDS             <1..687
                     /locus_tag="VFM00_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HET7497456.1"
                     /translation="EFWQEGSGDERSKNRYERQLLEAQLARLDLKTKPAKYIKVYNAD
                     EANNVRRNIATLRDSPLVAIVFNFLDMLAHGRSESDLLKELAPDESAFRSVMRSWFLH
                     SALFDALKWLAKQDGLVVVTTDHGAVLSRRSALVYGNRETSTNLRFKFGTNLSCEAKQ
                     AVHVKDPARFRLPADSPHKSYIIAKEDFYFVYPTRFHEYENKYRGSFQHGGISLEEMI
                     LPVAVMTPRG"
     gene            695..1198
                     /gene="tsaE"
                     /locus_tag="VFM00_04085"
     CDS             695..1198
                     /gene="tsaE"
                     /locus_tag="VFM00_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET7497457.1"
                     /translation="MPVEIRSLLHTEAETRAFGRHLGTLLRRGDWIALTGALGAGKTT
                     LVQGIVEGVHPGMRSRSPTYVLVEVYGEGPRIVHADLYRMAAAAEIETLGLADLAEGD
                     AVVLVEWADRALDRLPPDRLELELRYVPEGGRELRIRPQGSRWENTAHEGQLDRDRWS
                     HALYPGN"
     gene            1176..1862
                     /gene="tsaB"
                     /locus_tag="VFM00_04090"
     CDS             1176..1862
                     /gene="tsaB"
                     /locus_tag="VFM00_04090"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET7497458.1"
                     /translation="MRFILGIDTAGPRGSIAVAEEGAASQPVALPEGGHSSGLAPAIE
                     RLLAARGIRVADLAGIAVSAGPGSFTGLRIGLAWAKGAALGAGLPLFMIPSHEAAAHA
                     HRRSAPRFATLTQGERGHVMAALWDGCVEEGDDRARLLWGPEVVPEEDVPDRLRERAG
                     ADVPVAAATEALEEWVLDLGGTTLPSLPLGPAVAELGAIALAAGQRADLREAAPLYGR
                     APNARKPGAR"
     gene            1859..2344
                     /gene="rimI"
                     /locus_tag="VFM00_04095"
     CDS             1859..2344
                     /gene="rimI"
                     /locus_tag="VFM00_04095"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET7497459.1"
                     /translation="MTALARRGEPVRIRPLTVARLDEVLAIERVSFSDPWSREMFRSE
                     LMIGGGTYARLAERDGALVGYLCAVLVADEAHVGNLAVDPAERRQGVAQLLLDDLVEA
                     ARSRGARRVTLEVRESNENARKFYYKNEFIDIAIRKNYYRNPSEDAIVMLRTLPEGPS
                     A"
     gene            2341..3417
                     /gene="accD"
                     /locus_tag="VFM00_04100"
     CDS             2341..3417
                     /gene="accD"
                     /locus_tag="VFM00_04100"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET7497460.1"
                     /translation="MTWLRKAKEGLKARERRRDLPDGLWVKCEECGEILYHKELERNL
                     WTCGKCGYHFRISARAYVKILLDEGSFQERFSEVVSTDPLRFKDSKRYIDRLKKAREE
                     TGLSEAVLCGDGTIESHSCVIAIMDFAFLGGSLASAAGERIARSILLAIETRRPLIIL
                     SASGGARMFEGILSLMQMAKTNALLARLSDARIPYISVMTHPTTGGVTASFASVGDVI
                     LAEPKALIGFAGPRVIKQTINQELPEGFQRSEFLMKKGMVDQIVHRQELKKTLGYLLR
                     FFGATSVLPQKPRRRAGESLDRFRAASVAAPEPIGLSPSDNGHPAGAASSARAVPGST
                     NAAERPPAPESNPEPTPTETASQE"
     gene            3414..4724
                     /locus_tag="VFM00_04105"
     CDS             3414..4724
                     /locus_tag="VFM00_04105"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET7497461.1"
                     /translation="MTRTVGAPLTRRLDELYRFERSGMRLGLLGIERLLERGGRPDRA
                     FPSVLIAGTNGKGSTAAHLASILRAAGLKTGLYTSPHLVRFHERIRVDGAEISDLALE
                     EALERWWPRFEEERPSFFEAATALAFDHFARESVDMAVVEVGLGGRLDATNVLVPKAS
                     VITTISTDHAEILGPTLRRIAAEKAGIIKPAGTLVLGVRRAEPRSEILEAAVRQGARV
                     LLMGRDGRYAARGLTRAGTDVWIKTPSFAGLARTPLLGFHQARNAALAALAAEVALEG
                     RPAPAIARSIVEGIAATRWPVRAEVLGEDPPVLADVAHNAEGARALAETVAALFPGRK
                     VAVVAAFSRDKAHESILATLARVASRFTLTEFGGERATPASTLARVVPASHLTCEAVT
                     DPAQAVRGAVEWARDTGGVVVVTGSFYLMPEAMAALGHEVPRAI"
     gene            4714..>5106
                     /locus_tag="VFM00_04110"
     CDS             4714..>5106
                     /locus_tag="VFM00_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HET7497462.1"
                     /translation="MPFRRAGTGLPILLAALAALAATLAAPGRARAQPAETYRLQADR
                     LEGSLTGAENVYTAIRPTLTHGTTTVTGDSALVYRTREYVIFRGDVKIVDGTTTMWGD
                     EATYDKKQRLATLRGNVRIQERGSRITGS"
BASE COUNT          760 a         1764 c         1843 g          739 t
ORIGIN      
        1 gagttctggc aggaggggtc gggggacgag cggagcaaga accgctacga gcgccagctg
       61 ctcgaagccc agctcgcgcg gctggacctg aagacgaagc cggccaagta catcaaggtc
      121 tacaacgccg acgaggcgaa caacgtgcgg cggaacatcg cgaccttgcg cgactcgccg
      181 ctggtcgcca tcgtcttcaa cttcctcgac atgctggccc acgggcggtc ggagtcggat
      241 ctcctcaagg aattggcccc cgacgagtcc gcgttccgct cggtgatgcg ctcgtggttc
      301 ctccattccg cgctcttcga tgcgctgaag tggctggcca agcaggacgg cctcgtggtc
      361 gtgacgaccg accatggcgc ggtgctgagc cgccggtcgg cgctggtcta cggcaaccgg
      421 gagacctcga ccaatctgcg gttcaagttc ggaacgaatc tctcgtgcga ggcgaagcag
      481 gcggtccacg tgaaggaccc ggcccgcttc cgcctccccg ccgacagtcc gcacaagagc
      541 tacatcatcg cgaaggagga tttctatttc gtctatccga cgcgattcca cgaatacgag
      601 aacaagtacc ggggttcctt ccagcacggg ggaatctcgc tcgaggagat gatcctgccc
      661 gtcgcggtga tgaccccgcg cgggtaggcc cctgatgccg gtcgagatcc gatccctgct
      721 ccacaccgaa gccgagaccc gcgcgttcgg acgccatctg ggcacgctgc tccgccgcgg
      781 cgactggatc gcgctgaccg gcgctctggg cgcggggaaa acgacgctgg tgcaggggat
      841 cgtggagggg gtccatccgg gcatgcgctc gcggagcccg acctacgtgc tggtggaggt
      901 ctacggcgag gggccgcgca tcgtgcacgc cgacctctac cggatggcgg cggcggccga
      961 gatcgagacg ctgggcctgg ccgacctggc cgagggcgat gcggtggtgc tggtggaatg
     1021 ggcggaccgg gcactcgacc ggctgcctcc cgatcgcctg gagctggagc ttcgctacgt
     1081 ccccgagggg gggcgggagc tccgcatccg gccgcagggg agccggtggg agaacacggc
     1141 gcacgaggga caactggacc gcgaccgatg gagccatgcg ctttatcctg ggaattgaca
     1201 ccgccgggcc gcgcggctcg atcgcggtcg ccgaagaggg cgccgcaagc cagccggtcg
     1261 cgcttcccga gggcgggcac tcgagcgggc tcgcgcccgc gatcgagcgg ctgctggcgg
     1321 cccgcgggat tcgtgtggcg gatctcgcgg gtatcgcggt ctccgcgggg ccgggttcct
     1381 tcaccgggct tcggatcggc ctcgcttggg cgaagggtgc ggcgctggga gcgggccttc
     1441 cgcttttcat gatcccctcc cacgaggcgg cggcccacgc gcatcgaagg agcgcgcccc
     1501 gattcgcgac cctgacccag ggggagcggg ggcacgtgat ggcggcgctc tgggacggtt
     1561 gtgtcgagga gggggacgac cgggcccggc ttctctgggg gccggaagtc gttcccgaag
     1621 aggacgtgcc ggaccggttg cgcgagcggg cgggcgccga cgttcccgtc gccgccgcca
     1681 cggaagcgct ggaggaatgg gtgctcgatc tgggcggaac gacgctgccg tccctgccgc
     1741 tcggccccgc ggtcgcggag ctgggcgcga tcgcgctggc cgccgggcag agggccgatc
     1801 tccgcgaggc ggccccgctc tacggccgcg cgccgaacgc gcggaagccg ggggcgcgat
     1861 gacggcgctc gcccgacgcg gggagccggt ccggatccgg ccgctcaccg tcgcgcgcct
     1921 cgacgaggtc ctcgcgatcg agcgggtctc cttcagcgat ccctggtcgc gcgagatgtt
     1981 ccggtccgaa ttgatgatcg gcggcgggac ctacgcgcgg ctggccgagc gggacggagc
     2041 gctggtcggc tatctgtgcg ccgtgctggt cgccgacgag gcgcacgtcg gaaacctggc
     2101 cgttgacccc gccgagcggc ggcagggggt ggcccagctc ctcctggacg atctggtcga
     2161 agcggcccgg agccgggggg cccggagggt caccctggag gtacgggagt ccaacgaaaa
     2221 tgcccgtaag ttctattaca agaacgagtt catcgacatc gccatccgca agaactatta
     2281 ccgcaaccct agcgaggatg ctattgtcat gctccgaacc ctcccggagg gacccagcgc
     2341 atgacgtggc ttcgcaaggc caaggaaggg ctcaaagcta gagaacggcg gcgggacctg
     2401 cccgacgggc tctgggtcaa gtgcgaggaa tgcggcgaga tcctgtacca caaggagctc
     2461 gagcggaacc tctggacgtg cggcaagtgc ggctatcact tccgcatctc ggcccgtgcc
     2521 tacgtcaaga tcctcctcga cgagggctcc ttccaggagc gcttctccga agtcgtctcc
     2581 accgatcccc tccggttcaa ggattccaag cgctacatcg accgtctgaa gaaggcgcgg
     2641 gaggagaccg ggctttcgga ggcggtgctc tgcggcgacg ggacgatcga atcccactcc
     2701 tgcgtcatcg cgatcatgga cttcgctttc ctgggcggaa gcctggcgtc ggccgcgggg
     2761 gagcggatcg cccgcagcat cctcctcgcg atcgagacgc ggcggccgct gatcatcctc
     2821 tccgcctcgg gcggggcgcg gatgttcgag gggatcctct ccctcatgca gatggcgaag
     2881 acgaacgcgc tcttagcgag gctctccgac gcgcgcatcc cctatatctc ggtcatgacc
     2941 cacccgacca cgggcggcgt caccgcctcg ttcgcctctg tgggcgacgt gatcctggcc
     3001 gagccgaagg cgctgatcgg cttcgccggc ccgcgcgtga tcaagcagac gatcaaccag
     3061 gagcttcccg agggcttcca gcgctcggag ttcctgatga agaagggaat ggtcgaccag
     3121 atcgtgcacc ggcaggagct gaagaagacg ctgggctacc tgcttcgctt cttcggcgcg
     3181 acgtcggtgc tgccgcagaa gccgcggcgg cgcgccggcg agtcgctgga ccggttccgg
     3241 gcggcgtcgg tggcggcccc cgagccgatc ggcctttcgc ccagcgacaa cggccatccc
     3301 gccggcgcgg cgtcttccgc gcgtgccgtc ccgggcagca ccaacgcggc cgagcgcccc
     3361 cccgctcccg agtccaaccc cgagccgacc ccgaccgaga ccgcctccca ggaatgaccc
     3421 gcaccgtggg cgctccgctc acgcggcgcc tcgacgagct ctatcggttc gaacgcagcg
     3481 gcatgcggct cggcctcctc ggcatcgagc ggctgctgga gcgcggcggc cgtcccgacc
     3541 gcgcgttccc ctcggtgctg atcgcgggaa ccaacggcaa agggtcgaca gcggcgcatc
     3601 tggcctcgat cctccgcgcc gcgggtttga agaccggcct ctacacctcg ccccacctgg
     3661 tgcgattcca cgagcggatc cgcgtggacg gcgccgagat ctccgacctg gccctggaag
     3721 aggcgctgga gcggtggtgg ccgcgcttcg aggaggagcg cccctccttc ttcgaggccg
     3781 cgacggcgct ggcgttcgat cacttcgcgc gcgaatcggt ggacatggcg gtggtcgagg
     3841 tcgggctggg cgggcgcctg gacgccacca acgtcctcgt cccgaaggcc tccgtcatca
     3901 ccaccatctc gaccgatcac gccgagatcc tgggtcccac gctccggcgg atcgcggcgg
     3961 agaaggcggg gatcatcaag cccgccggca cgctggtgct gggggtccgc cgcgccgagc
     4021 cgcgctcgga gattctcgaa gcggccgtcc gacagggagc gcgcgttctc ctgatggggc
     4081 gcgacgggcg ctacgcggcg cgcggcctga cccgcgccgg caccgacgtc tggatcaaga
     4141 cgccctcctt cgcgggcctg gcgcgcacgc cgctcctggg atttcaccag gcgcggaacg
     4201 cggcgctggc cgcgctcgcg gccgaagtgg cgctggaggg ccgccccgcc cccgcgatcg
     4261 cgcggtccat cgtcgagggg atcgccgcga cgcgctggcc cgtccgggcc gaggtgctgg
     4321 gcgaggatcc tcccgtgctc gcggacgtgg cccacaacgc ggagggggcg cgcgccctgg
     4381 ccgagacggt ggcggcgctc tttccgggac ggaaggtggc ggtggtcgcc gcgttctcgc
     4441 gcgacaaggc gcacgagtcg atcctggcca cgctcgcgcg cgtcgcgagc cggttcacgc
     4501 tgaccgaatt cggcggcgag cgcgcgacgc cggcgtcgac gctggcgcgc gtcgtccccg
     4561 cgagccacct gacctgcgag gcggtgaccg accccgccca ggccgtgcgc ggtgctgtgg
     4621 aatgggcgcg cgacacgggc ggcgtggtcg tggtcacggg atccttctat ctcatgccgg
     4681 aggcgatggc ggcgctgggg cacgaggtgc cgcgtgccat ttaggagggc ggggacgggg
     4741 ctcccgattc tcctcgccgc gctggccgcg ctggccgcga ccctcgccgc gcccgggcgg
     4801 gcgcgcgccc agccggcgga gacctaccgg ctgcaggccg accgcctcga gggctcgctc
     4861 accggagccg agaacgtcta taccgcgatc cgtcccacgc tgacccacgg cacgaccacc
     4921 gtcaccggcg actccgcgct cgtctaccgg acgcgggaat acgtgatctt ccgtggcgac
     4981 gtgaagatcg tggacggcac gaccacgatg tggggcgacg aggccaccta cgacaagaag
     5041 cagcggctcg ccacgctgcg ggggaacgtc cgcatccagg agcgtggcag ccgcatcacc
     5101 ggcagc
//
LOCUS       DASSLZ010000111         4718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_795804, whole genome shotgun sequence.
ACCESSION   DASSLZ010000111 DASSLZ010000000
VERSION     DASSLZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795804"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VFM00_04115"
     CDS             complement(<1..1051)
                     /locus_tag="VFM00_04115"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7497463.1"
                     /translation="MSRTRYDVAILGAGPGGYVAAIRAGQLGLKTALIEKDRPGGTCL
                     HWGCIPTKALIETAEVLLLCRRAGEFGVRVAEATLDLKAAQERKERVVRKNFMGTEAL
                     LRKNRVETIKGAGTMVAPDRIRIEGDGGGAIEAGAVIVATGSVVGSLPLATVDHRVVL
                     SSDDVLGLDRVPASMLVIGAGAVGVEFASIYHTFGTKVTLVEREPRLLPLEDAEISEA
                     LLRSFRRQGMEVFVGAELAPVRVEGDGAWVEVRSHDAAKRFRVERVLMAAGRRPATEG
                     IGLEAIGVAMDRGYVNVDCLQRTNVGGVYAIGDVTKSAALAHVASHEGITAVEAIAGK
                     NPHRVNHDAIPSCTYC"
     gene            complement(1048..1824)
                     /locus_tag="VFM00_04120"
     CDS             complement(1048..1824)
                     /locus_tag="VFM00_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET7497464.1"
                     /translation="MIGYPVLILVILGILLLTSAIKVLREYERAVVFRLGRAVGAKGP
                     GLIFLIPIVDKMVRIPLRTVAMDVPPQDVITRDNVTVKVNAVIYFRVLDANRAVLEVE
                     NYLYATSQIAQTTLRSSLGESDLDQLLSNRDEINLKLQQVIDRHTEPWGIKVSTVEVK
                     NVDLPQEMQRAIARQAEAERERRAKVINAEGELQASQKLLEAAQVIAREPIALQLRYL
                     QTLAEIATENNSTTVFPIPIDLFEPILKARAAALTAGPRT"
     gene            complement(1837..3180)
                     /locus_tag="VFM00_04125"
     CDS             complement(1837..3180)
                     /locus_tag="VFM00_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HET7497465.1"
                     /translation="MTLPRAGALLAALIFLILGFAPAAASAKGPPSAPRTKAEPASPA
                     APEIDILTIDGAIQPITAQVIVRAIDGAEKHDREMLIIRLDTPGGLDTSMREIIKRIL
                     VSRVPVVVYVAPSGSRAASAGTFIAMAAHVAAMSPGTSIGAASPVNLGGGIADTTMAH
                     KVRNDAISYIRSLAAQRGRNVDWAEKAVREGGSLPESDALKMNVVDLVARDDDDLLRQ
                     LDGRKVTIGGETRTLHTQNAVRHAFTMSWRQGLLSKIVDPNVAYILFMLGFYGLIFEL
                     SSPGSILPGVVGGICILLAFLAFQTIPINMTGLALILFAMTLFIVDLKVQSHGILTAG
                     GIVSLVLGSLLLIGGDAGMARISYSVIGTVVVATVVFFVFVLGAAFRAQRRKPMTGRE
                     GLIGERGTALTDLEPGGRVFVHGEYWEAESSEHIERGSAVVVDQVDGMRLRVHKA"
     gene            complement(3177..3557)
                     /gene="panD"
                     /locus_tag="VFM00_04130"
     CDS             complement(3177..3557)
                     /gene="panD"
                     /locus_tag="VFM00_04130"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HET7497466.1"
                     /translation="MRLSVFKSKIHRAVVTEANLNYEGSVTIDADLMEAADILPHEQV
                     QVLNVNNGERFDTYAIRGARGSGVICLNGPAARLAQVGDKVIILTYAWMEREELERHT
                     PRVVMVDERNRALKTEAAGGRGSR"
     gene            complement(3548..4525)
                     /locus_tag="VFM00_04135"
     CDS             complement(3548..4525)
                     /locus_tag="VFM00_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET7497467.1"
                     /translation="MRATLAGQTTDRPPVSLWRHFYDREGSAEDLFHAMVQFQETYDW
                     DLMKVNPRASYHVEDWGVRTQRPGTGPLDKPKVVQSVIKKPEDWDRIRPLDVTRGVLG
                     EHLDAEERIAARVQGEVDWVMTVFNPISIAADLVYDDAMFVEHLRRHGERVHGALRAI
                     TETFAAFTRETMARGASGIFFATTDWGNDTRIDRETVEEFGRPYDLRVLAEASAAPLN
                     ILHVCGPGAFLRELDDYPVQVLSWDAHEKGNPSIRAMRGVTEKTLLTGISHEGTFVNG
                     PAKALEDEARAAVAASGGTRFILGPGCAVPAAAPQEHYGIVREAALACA"
     gene            4534..4718
                     /locus_tag="VFM00_04140"
                     /pseudo
     CDS             4534..4718
                     /locus_tag="VFM00_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304321.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT          713 a         1657 c         1591 g          757 t
ORIGIN      
        1 ggcagtaggt gcagctcggg atggcgtcgt ggttcacgcg atgcgggttc ttccccgcga
       61 tcgcctcgac cgcggtgatc ccctcgtgcg aggccacgtg cgcgagcgcc gcgctcttcg
      121 tgacgtctcc gatcgcgtag acgccgccca cgttcgtgcg ctgcaggcag tccacgttca
      181 cgtagccgcg gtccatggcg acgccgatcg cctcgagccc gatcccctcc gtcgcgggcc
      241 gccgccccgc cgccatcagc acgcgctcca cccggaagcg cttcgcggcg tcgtgcgacc
      301 ggacctcgac ccaggccccg tccccttcga cgcgcaccgg tgccaactcg gcgccgacga
      361 agacctccat cccctgccgg cggaaggagc gcagaagggc ctcggagatc tcggcgtcct
      421 cgagcggcag gaggcgcggc tcgcgctcca ccagggtcac cttggtcccg aacgtgtggt
      481 agatcgaagc gaactcgacg ccgaccgcgc cggcgccgat cacgagcatc gacgccggca
      541 cgcggtccag tccgagcacg tcgtcgctcg agagcacgac acgatggtcc accgtcgcga
      601 gcggcagcga tccgacgacc gagccggtcg cgacgatcac ggccccggcc tcgatcgctc
      661 cgccgccgtc gccctcgatc cggatccggt ccggagcgac catcgtcccc gcgcccttga
      721 tcgtctcgac gcggttcttc cgcaggagcg cctcggtgcc catgaagttc ttccggacca
      781 cgcgctcttt ccgctcctgc gccgccttga ggtcgagcgt cgcctcggcc acgcgcacgc
      841 cgaattcccc ggcgcgccgg cagaggagca gcacctccgc cgtctcgatc agcgccttgg
      901 tcgggatgca cccccagtgg aggcaggtgc cgcccggccg gtccttctcg atcagcgccg
      961 tcttcagccc gagctgcccc gcgcggatgg cggccacgta gcctcccggg ccggcgccga
     1021 gaatggcgac gtcgtaccgg gttcgactca cgtgcgaggt ccggcggtca gcgccgcggc
     1081 ccgcgccttc aagatcggct cgaacagatc gatcgggatc gggaagacgg tcgtggagtt
     1141 gttctcggtc gcgatctccg cgagcgtctg gagatagcgc agctggagcg cgatcggctc
     1201 gcgcgcgatc acctgggccg cctcgagcag cttctgcgag gcctggagct cgccctcggc
     1261 gttgatcacc ttggcccgcc gctcgcgctc ggcctcggcc tggcgcgcga tggcgcgctg
     1321 catctcctgc ggcaggtcga cgttcttcac ctcgaccgtg ctcaccttga tcccccacgg
     1381 ctccgtgtgc cggtcgatca cctgctgcag cttgaggttg atctcgtcgc ggttggagag
     1441 gagctgatcc aggtccgact cgccgagcga gctccgcagc gtggtctggg cgatctgcga
     1501 ggtggcgtag agatagttct ccacctcgag cacggcgcgg ttggcgtcga gcacgcggaa
     1561 atagatcacc gcgttcacct tgacggtcac gttgtcgcgc gtgatcacgt cctgcggcgg
     1621 cacgtccatc gccaccgtcc ggagcgggat ccgcaccatc ttgtccacga tcgggatgag
     1681 gaagatcagt cccggcccct tggcgccgac ggcgcgcccc aggcggaaca ccacggcgcg
     1741 ctcgtattcg cgcagcacct tgatggcgct ggtgaggagg aggattccga ggatgacgag
     1801 aatcaacacc ggataaccga tcatcacggc tccttcctag gctttgtgca cccggagccg
     1861 catgccgtcg acctgatcga cgacgaccgc cgagccccgt tcgatgtgct ccgaagactc
     1921 cgcttcccag tactcgccat gcacgaacac gcgcccgccc ggctcgaggt cggtcagggc
     1981 ggtgccccgc tcgccgatca gcccctcgcg cccggtcatc ggcttccgcc gctgggcccg
     2041 gaacgccgcg ccgagaacga agacgaagaa gaccacggtg gcgacgacca ccgtaccgat
     2101 caccgaatag gagatccgcg ccatgcccgc atcccctccg atcagtagaa gcgagccgag
     2161 gacgagggac acgatgcccc ccgcggtcag gatcccgtgg ctctggactt tgaggtcgac
     2221 gatgaaaagg gtcatcgcga acaggatgag cgcgagaccg gtcatgttga tcgggatggt
     2281 ctggaacgcg aggaaggcga gcaggatgca gatgcccccg acgacgccgg gaaggatcga
     2341 gcccgggctg gacagctcga agatcagccc gtagaacccg agcatgaaca ggatgtaggc
     2401 gacgttcgga tcgacgatct tggagagcag tccctggcgc cacgacatcg tgaaggcgtg
     2461 gcgcacggcg ttctgggtgt gcagcgtgcg ggtctcgccg ccgatcgtga ccttccggcc
     2521 gtcgagctgg cggaggagat cgtcgtcgtc gcgcgcgacc aggtccacga cgttcatctt
     2581 cagggcgtcg ctctcgggga gcgaaccccc ttcgcgcacc gccttctcgg cccagtccac
     2641 gttccggccc cgctgggccg ccagggagcg gatgtaggag atcgcgtcgt tccgcacctt
     2701 gtgcgccatg gtcgtgtcgg cgatgccgcc gcccagattc accggcgagg ccgcgccgat
     2761 cgaggtgccc ggcgacatgg cggcgacgtg cgcggccatc gcgatgaagg ttcccgcgct
     2821 cgccgcgcgg ctccccgagg gcgcgacgta gaccaccacg gggacgcgcg agaccaggat
     2881 gcgcttgatg atctcgcgca tgctggtgtc cagtccgccc ggcgtgtcga ggcggatgat
     2941 cagcatctca cggtcgtgct tctccgcgcc gtcgatggcg cgcacgatga cctgcgccgt
     3001 gatcggctgg atcgcgccgt cgatggtgag gatgtcgatc tcgggcgctg ccgggctcgc
     3061 cggctcggcc ttcgtccggg gagcggacgg aggccccttg gccgaagcgg ccgccggagc
     3121 gaaaccgagg atgaggaaaa tgagcgccgc gaggagagcg ccggcgcgcg ggagtgtcac
     3181 cgggagcctc gcccgccggc cgcctccgtc ttgagagccc ggttccgctc gtcgaccatg
     3241 acgacgcggg gcgtgtgccg ctccagctct tcgcgttcca tccacgcgta ggtgaggatg
     3301 atgaccttgt cgcccacctg cgccaggcgg gcggccggtc cgttgaggca gatcacgccg
     3361 ctcccgcgcg cgccgcggat ggcgtaggtg tcgaagcgct ccccgttgtt cacgttgagc
     3421 acctgcacct gctcgtgcgg caggatgtcg gcggcttcca tgaggtcggc gtcgatcgtg
     3481 accgatccct cgtagttgag gttcgcctcg gtcaccacgg cgcggtggat cttggacttg
     3541 aaaacgctca ggcgcatgcg agcgcggcct cccgaacgat gccatagtgc tcttgcggag
     3601 ccgccgccgg taccgcgcag ccgggcccca ggatgaaacg cgtcccgccg gatgcggcca
     3661 cggcggcgcg cgcctcgtcc tccagcgcct tcgccggacc gttcacgaac gttccctcgt
     3721 ggctgatgcc ggtgagcagg gtcttctcgg tcacgccgcg catggcgcgg atcgagggat
     3781 tccccttctc gtgggcgtcc cacgacagga cctgcaccgg atagtcgtcc agctcgcgca
     3841 ggaaggcgcc cgggccgcag acgtggagga tgttgagcgg ggcggccgat gcttcggcga
     3901 gcacgcgaag atcgtaagga cgcccgaact cctcgaccgt ctcccggtcg atccgcgtgt
     3961 cgttgcccca gtcggtggtc gcgaagaaga tccccgaggc gccgcgcgcc atcgtctcgc
     4021 gcgtgaacgc ggcgaaggtc tcggtgatgg cgcggagcgc cccatggacg cgctcgccgt
     4081 ggcggcgcag atgctccacg aacatcgcgt cgtcgtagac caggtcggcg gcgatcgaga
     4141 tcgggttgaa cacggtcatc acccagtcca cctcgccctg cacccgcgcg gcgatccgct
     4201 cctcggcgtc gaggtgctcg ccgaggacac ccctcgtgac gtccagcggg cggatccggt
     4261 cccaatcctc cggcttcttg atgacgctct ggaccacctt cggcttgtcc agcggcccgg
     4321 ttcccggccg ctgggtgcgc acgccccagt cctcgacgtg atacgaagcg cgggggttca
     4381 ccttcatgag atcccagtcg taagtctcct ggaattgcac cattgcgtgg aaaagatcct
     4441 cggccgagcc ctcgcggtcg tagaagtggc gccacaggga gacggggggc cggtccgtcg
     4501 tctggcccgc cagcgtggct cgaatccgtt cacgtcgcgt catgatgggt accgttcgcc
     4561 gtcgacgatc accagaccgc aaacgcgcgc ttcctcgccg accgtggcgc ggcagttctg
     4621 ctgccacaga cagaactcac ggctgagcgg ctcgctgagc ttattcggaa gatggaccgc
     4681 acgacgctgc tgcagatggc gcggaatgca cgggctct
//
LOCUS       DASSLZ010000112         7441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1685071, whole genome shotgun sequence.
ACCESSION   DASSLZ010000112 DASSLZ010000000
VERSION     DASSLZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685071"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFM00_04145"
     CDS             <1..755
                     /locus_tag="VFM00_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET7497468.1"
                     /translation="AEAVKLAVASLTPEVLLFGATGQGRDLSARAAARLGAGLASDCV
                     GFEWKDGGLVARRPIFAGKAFARVAFGAARPCVASVRPNTFQPAAPDAGRSASVEPIA
                     LGDGVKVRARVVSFEATEGGVADLTEANIIVSGGRAMGGPENFELLRKLCKVLGATLG
                     ASRAAVDAGWIDHSYQVGQTGKVVNPTLYFAVGISGAIQHLAGMSSARTIVAINKDKE
                     APIFKVATYGVVGDLYQIVPALTEEFAKLLKE"
     gene            797..1456
                     /locus_tag="VFM00_04150"
     CDS             797..1456
                     /locus_tag="VFM00_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7497469.1"
                     /translation="MSGHPAYETYWKRKELLKRSFPRFPVRRWWETDGLCDIERVYFD
                     AIREAPSLLDVGAGDLRVMRKLQAAGYRGEYHTQDIGDGGPWTYADLSEVRRPYGAIL
                     CLDVLEHLALPDGIALLNRLVSLLSPGGALVLQTPNAAYLPEPRSWDMTHVHTYALHD
                     LWAYFTVDGLEVGGYRVVLGPEREGLLESFRSGMQHWVKQKLLGCDFANNVAVIARKR
                     A"
     gene            1585..4122
                     /locus_tag="VFM00_04155"
     CDS             1585..4122
                     /locus_tag="VFM00_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7497470.1"
                     /translation="MAANALPVTIPPARAQEAYYHKPMPEVEKVLEARPIPAVWLSPA
                     RDVLLIADRPGYPPLADLAAPKVKLAGVRVNPVTNGPHRQSYFLGLTVKSLADAPEPA
                     DAKSRRGDHRSGGAWGGRGGATGGRGGATGGRRVTLPDSIRISNPLWSADGKWVAFTN
                     TTKDSLELWVLDVAQAAARRVPRVHLNGALGWTYLWMPDQRTLLVKMVDPDRGPPPRL
                     PPVPTGPDVQEGSGKHGIGSTYERRDVLETAHDEDLFDYYGTSALAFVDVTGGTVTRF
                     GKADLFGSIQPSPDGEHFIVERIHRPYSHLHPWWRFPREVEIWDRHAQVERRLASLPL
                     ADQVPSEGVPTGPRFYSWRPTAPATLFYVEALDQGDPDVKVPHRDRLLTWAAPFPGEA
                     SEVMRLRERFAGIAWTDSSGIALVDEYDRERRWETTYQADLSVPGAATRVIWDLSENE
                     KYKAPGAPLFRPLANGFSVIDRDGASIYLAGAGATPSGDRPFLDRFDLKTLQTERLFR
                     CDPDVYESFMGWAGTGRSRFLTRRESPKEPPNTFLRVLGAPIAGKDGEAVWTSALAPV
                     TQFLDPTPELRGITKRIVTYKRPDGTPLSFTLYLPPGYKSGRLPTVLWAYPLEYSDEG
                     TAGQVSGSDRRFTQITGASPLFFTLDGYAVLDNVAMPVIGHPDSVYNVYLGQLVANAK
                     AAIDKAVELGVTDRDRVGIAGHSHGAFMTANLLAHSDLFRAGIARSGAYNHTLRPFGF
                     QSERRTYFQAPESYIRLSPELHADKINEPILIIHGEDDANPGTVPLQSDLLYRAVVGT
                     GGTARLVMLPLESHSYEAKESIEETLWEMMTWFARYVKEAPDRASVK"
     gene            complement(4162..5592)
                     /locus_tag="VFM00_04160"
     CDS             complement(4162..5592)
                     /locus_tag="VFM00_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497471.1"
                     /translation="MHEYVLTHLSDAILLRDLKALVRQDRATTVMLLAHIAEVDSRRL
                     YAPAGYSSMFAYCTEELGLSEDAAYKRIRAARAARRFPVLFKEMAEGRLHMAAVCVLS
                     PHLTSENLEELTQAASHRRKSEVEEWLIHRFPGAVSASRECVTSIRPLDVSRMLTGSM
                     DQLVLGPVPADPQATPHPETPSLPSNEQLVPGPVVGGPRNERDGAPSPKDLIEAPLPD
                     LASRASEMERLPKRYLVRLTIDEATHEKLRRAQAMLSHAVPGGDVAEVLGRALDALIR
                     QLETRKIGSPSGGKVKTREAEPPREASPKPRSSNGAGAAGSRYIPARVRRAVWERDQG
                     RCTFVTHAGHRCSARRLLEFDHIEPVARGGRSTVDGLRLRCRAHNQYAAERAFGAEFM
                     RQKREARREQSVRREASKSQPNMVREPSCSWQVLAPGRVGVRRDVTIAGLMQTELAPG
                     RVGVAPRPRCASAAHEHVLEDVDRAR"
     gene            complement(6034..6561)
                     /locus_tag="VFM00_04165"
     CDS             complement(6034..6561)
                     /locus_tag="VFM00_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7497472.1"
                     /translation="MASSLDGLVIRNYGSSESIPEMTSLLRDAYAHLARQGLRYVATH
                     QDDTITARRLGRGFPLVVYLDGHLVGTATLYPPRPDSPVAWYRRPEVFYFGQFGVRPE
                     LQRQGIGVRILRALEDRARARGATELACDTAEQAEHLRSWYAREGFRMVGTTKWPVTN
                     FQSAVLSKTIVMPGS"
     gene            complement(6663..7139)
                     /locus_tag="VFM00_04170"
     CDS             complement(6663..7139)
                     /locus_tag="VFM00_04170"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7497473.1"
                     /translation="MDASTHSASIREFRSEDEPALFAIHDRSRPDELVGSCDPRGFIP
                     LAEDFRSLEDFRMSRKWVAVDGDRAVGFAGVRDNYISWLYIEPEYYGRGIGRRLLRAA
                     MEAAGPDAWTIALENNTRARKLYESEGFLPALTFDGENNGYPCRCVKLMLRPPKKG"
     gene            complement(7141..>7441)
                     /locus_tag="VFM00_04175"
     CDS             complement(7141..>7441)
                     /locus_tag="VFM00_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497474.1"
                     /translation="GDPVRGSLLYATVRLKGAPPDTIPPTAGKVYLWVVHGSDVWSAT
                     LALGPIDPDGTRTYFVSGGPRWGPGIDVDVVIGVRSSPSDVALVLRRAVPILMTS"
BASE COUNT         1214 a         2522 c         2456 g         1249 t
ORIGIN      
        1 acgccgaggc cgtgaagctc gcggtggcgt cgctgacccc cgaggtgctg ctgttcggcg
       61 cgaccggcca gggacgcgat ctctcggcgc gcgccgcggc gcgcctgggc gcgggtctcg
      121 cgtccgactg cgtgggcttc gagtggaagg acggcggcct ggtcgcgcgc cggccgatct
      181 tcgcgggcaa ggcgttcgcg cgcgtcgcgt tcggcgccgc gcggccgtgt gtggcatcgg
      241 tccggcccaa taccttccag cccgccgcac ccgatgcggg gcggagcgca tcggtcgaac
      301 cgatcgcgct cggcgacggg gtgaaggtgc gcgcccgcgt cgtgtccttt gaggccaccg
      361 agggcggcgt ggcggacctc acggaagcca acatcatcgt gagcggcggc cgcgccatgg
      421 gcggccccga gaatttcgaa ctgctgcgga agctctgcaa ggtgctcggc gccacgctgg
      481 gcgcctcgcg cgccgcggtc gacgccggct ggatcgatca cagctatcag gtgggccaga
      541 ccgggaaggt cgtgaatccc acgctgtatt tcgccgtggg aatttcaggc gccattcagc
      601 atctggccgg tatgtcctcc gcgagaacga tcgtcgcgat caacaaggac aaagaagctc
      661 ctatcttcaa agtggccacc tatggcgtgg tcggcgatct gtatcagatc gtccccgcgc
      721 tcaccgagga gttcgcgaag ctgttgaagg agtagttcgg gcgccgttcg catccctccc
      781 aaggaggcag gtccggttgt ccggccatcc ggcgtacgag acctactgga agcgcaagga
      841 gctgttgaag cgctcctttc cgcgattccc cgtccggcgg tggtgggaaa ccgacgggct
      901 gtgcgacatc gagcgcgtct acttcgatgc catccgcgaa gccccttccc tcctggacgt
      961 cggcgccggc gatctccgcg tgatgcggaa gctccaggcg gcaggctatc gcggcgaata
     1021 ccacacgcag gacatcggcg acggcgggcc gtggacctat gccgatctct ccgaagtgcg
     1081 gcgaccgtat ggcgcgattc tctgcctcga cgttctggaa catctcgcgc tgcctgacgg
     1141 gatcgcgctc ctgaatcgcc tggtgagcct gctctccccc ggcggcgcgc tcgtgctcca
     1201 gacgccgaac gccgcgtatc tccccgagcc tcggtcctgg gacatgacgc atgtacacac
     1261 ctacgccttg cacgatctct gggcctattt caccgtggat gggctggagg tgggcgggta
     1321 tcgcgtggtt ctgggccccg agcgagaggg acttctggag tcgttccgtt ccggcatgca
     1381 gcattgggtg aagcagaagc tgctgggctg cgacttcgcg aacaacgtgg ccgtcatcgc
     1441 gcggaagcgc gcgtgataga gtgggggatg ccgatgcgca tcccgtctca ttccccattt
     1501 cgccggcgcg gcccggtcca ggccgttggc gcgaaccgcc gccttgcttt cgctgcagcg
     1561 cccctcttcg ccacggcgac cctcttggca gcgaacgctc ttcccgtcac catccctccc
     1621 gcgcgcgcgc aggaggcgta ctaccataag ccgatgcccg aggtggagaa ggtgctggag
     1681 gcgcggccca ttcccgccgt ttggctgagt cccgcgcgcg acgtgctcct gatcgccgac
     1741 cggccgggct atccgccgct ggccgatctc gccgcgccga aagtaaagct ggccggcgtg
     1801 cgcgtgaatc ccgtcaccaa cggtccccac cggcagagct acttcctggg gctcacggtg
     1861 aagtcgctgg ccgacgctcc cgagcccgcc gacgccaagt ctcgtcgcgg ggatcatcgg
     1921 agcggcggcg cgtggggcgg gcgcggcggc gcgaccggcg ggcgcggcgg cgcgaccggc
     1981 gggcgccgcg tgacgctccc cgacagcatc cgcatcagca atccgctctg gagcgcggac
     2041 ggcaagtggg tcgctttcac gaacaccacg aaggacagtc tggagctctg ggtgctcgac
     2101 gtggcgcagg ccgcggcgcg gcgcgtcccg cgcgttcacc tgaacggcgc gctcggatgg
     2161 acctatctct ggatgccgga ccagcgaacg ctgctcgtga agatggtcga ccccgaccgt
     2221 ggtccgcctc cgcgcctgcc gccggtcccg accggcccgg acgtgcagga ggggtcgggc
     2281 aagcacggca tcgggagcac gtacgagcgg cgcgacgtcc tcgagaccgc ccacgacgag
     2341 gacctgttcg actactacgg gacgtcagcg ctcgcgttcg tggatgttac gggcggcacg
     2401 gtcacccgct tcggcaaggc cgatctcttc ggctccattc agccctcccc cgacggcgag
     2461 cacttcatcg tcgagcggat ccaccgcccc tattcccatc tccatccgtg gtggcgattc
     2521 ccgcgcgagg tggagatctg ggaccgtcac gcgcaggtgg agcggcgtct cgccagcctg
     2581 ccgctggcgg accaggttcc ttccgaaggc gtgccgacgg gcccacggtt ttactcctgg
     2641 cgccccaccg cgcccgcgac gctgttctac gtcgaggcgc tggaccaggg cgatcccgac
     2701 gtgaaggtcc cgcaccgcga ccgtcttctt acgtgggccg ctccgttccc gggcgaagca
     2761 tccgaggtca tgcgcctgag ggagcgcttc gccgggattg cctggaccga ttcctccggc
     2821 atcgctctgg tcgacgaata tgatcgcgag cgccgctggg agacgacgta ccaggcggat
     2881 ctgagcgtcc ccggcgccgc gacacgcgtg atctgggacc tgagcgagaa cgaaaagtac
     2941 aaagcgcccg gcgcgcccct gttccggccg ctcgcgaacg gattctcggt gatcgatcgc
     3001 gacggggctt cgatctatct cgccggtgcc ggcgcgacgc cctcgggcga tcgtccgttc
     3061 ctggatcggt tcgacctcaa gacgctgcag accgaacggc tgttccggtg cgaccccgac
     3121 gtctacgaat cgttcatggg ttgggccgga accggccgat cgcgattcct gacgcggcgc
     3181 gagagtccga aggagccgcc gaacacgttc ttgcgcgtcc tgggagcccc gatcgcgggg
     3241 aaggacggag aggccgtctg gacttccgcg ctggctcccg tcacccagtt cctcgacccc
     3301 acgcccgaac ttcgcggcat caccaagcgc atcgtgacgt acaagcgtcc cgacggcacg
     3361 ccgctctcgt tcacgttgta cctgccgccc ggttacaagt cgggccgtct gcccacggtg
     3421 ctctgggcat atcccctgga atactccgac gaagggacgg ccggccaggt gagcggctcc
     3481 gaccggcggt tcacgcagat cacgggcgcc tcgccgctct tcttcacgct cgacggctac
     3541 gcggtgctgg acaacgtggc gatgccggtg atcgggcacc ccgacagcgt gtacaacgtg
     3601 tacctcggtc agctcgtcgc caacgcgaag gcggcaatcg acaaggccgt ggagctgggc
     3661 gtgaccgacc gggatcgcgt cggcatcgcc ggacacagcc acggcgcgtt catgaccgcg
     3721 aacctgctcg cgcactcgga tttgttccgc gccggaatcg cgcggagcgg cgcctacaac
     3781 cacaccctgc ggccgttcgg tttccagtcg gagcgccgca cgtacttcca ggcgccggag
     3841 tcgtacatcc ggctatcgcc ggaactgcac gccgacaaga tcaacgagcc gatcctgatc
     3901 atccatggcg aggacgacgc caaccccggc accgtgccgc tgcagtcgga tctgttgtat
     3961 cgcgccgtgg tcggcactgg cggcacggcc cgactcgtga tgctcccgct ggagtcgcac
     4021 tcgtacgaag ccaaggaatc gatcgaggaa acactctggg agatgatgac ctggttcgcg
     4081 cgatacgtga aagaagcgcc ggaccgggcc agcgtgaagt agcagtcgcc cgcgctaccg
     4141 cacggcctgc aggcgcggtc gtcaccgcgc tcggtcaaca tcttccagaa catgttcatg
     4201 tgccgcgctg gcacaacgcg gtcgaggggc gacgcctact cgccccgggg cgagttctgt
     4261 ttgcattaga cccgcgatcg tgacgtcccg tctcacgcca actcgccccg gggcgagtac
     4321 ctgccacgaa catgacggct cacgaaccat gttcggctgc gacttcgatg cctcacgacg
     4381 cactgattgt tcccgacgag cctcccgctt ctgtctcatg aactccgccc cgaacgcccg
     4441 ctcggccgcg tactgattgt gcgcccggca acgcagtcgc aagccatcaa ccgtcgacct
     4501 tcctccgcgc gcaaccggct cgatatggtc gaattccagc aagcgtcgag ctgaacagcg
     4561 atggccggca tgcgtcacga aggtgcatcg gccctgatcg cgctcccata ccgcgcgccg
     4621 cacgcgcgcc gggatgtaac gcgatccggc cgcgccggcg ccattcgacg atcgaggctt
     4681 cggactcgcc tcgcgaggag gctccgcctc gcgggttttg acttttccgc ctgaaggcga
     4741 tccgatcttt cgtgtttcga gctgacggat cagagcgtcg agagcgcggc cgagcacctc
     4801 ggcaacatct ccgccgggga cggcatggct cagcatggcc tgggctcgtc gcagcttctc
     4861 gtgagtggcc tcatcgatcg tgagacggac aaggtagcgc ttcgggagcc tctccatctc
     4921 tgaggctcta ctggccaggt cgggcaaggg cgcttcgatc aagtctttgg gcgacggcgc
     4981 cccatcacgc tcattgcgcg ggccgcctac aactggtcct gggaccagtt gttcattgga
     5041 tggaaggctc ggagtctccg gatgtggggt tgcctgcggg tcggcgggaa ctggtcccag
     5101 gaccagttga tccatggagc ccgtcagcat ccgagagacg tcgagaggtc ggatggacgt
     5161 gacgcactct cttgaagccg agacggctcc cggaaagcga tggatgagcc attcctcgac
     5221 ctcggacttg cgccgatggc tggccgcttg ggtgagctcc tcgagatttt cggacgtaag
     5281 gtgaggactc agaacgcaaa cagccgccat gtggagccgc ccctctgcca tttccttgaa
     5341 gaggacaggg aatcgtcgag cggcccgtgc ggctcggatc cgtttgtagg cggcgtcctc
     5401 ggagaggcca agctcttctg tgcagtacgc gaacatagat gagtagcctg ccggtgcgta
     5461 gaggcggcgt gagtcaacct ctgcaatgtg ggccagcagc atgaccgttg tcgcccgatc
     5521 ctgacgtacc agggccttca aatcacggag cagaatggca tcgctgagat gggtcaacac
     5581 gtactcgtgc atgggaactc ctgtctcggg gcaggtcgca cgatcggacc gggccgacgc
     5641 ccggttccgc tggggttctt cgattagaac acgggtgatt tctgaagcga tgggagggcc
     5701 gacgtaaaac ggtgagttgg ggttggcgtc tctggccttg gccccgaaga tcgcagccct
     5761 ggtgccttgc taggacgctt gcatcgcgat cagcgaaaag ggttgaagat cgagcgaagt
     5821 ggagtggaga tttcggtcaa tagaattgtt tacaagaagg atgcgggagt gagtcattcc
     5881 gcgaagcgct ttcatcgacc aagtcgtgcg gcacctcggc gcgacacctc ggcgcgacac
     5941 ctcggcgcgg cctctcggcg cggcctctcg gcgcggcctc tcggcgcggc acctcggcgc
     6001 ggctcctcgg cgcggctcct cggcgcggcc ccttcacgac ccaggcatga cgatcgtctt
     6061 cgacaacacc gcgctctgaa agttcgtcac cggccacttc gtcgttccca ccatgcgaaa
     6121 tccctcgcgc gcgtaccacg accggagatg ctccgcctgc tccgccgtgt cgcacgccag
     6181 ctccgtggcg ccgcgtgcac gcgcgcgatc ctccagcgcg cggaggatgc gcactccgat
     6241 tccttgccgc tgaagctcgg ggcggacgcc gaactggccg aagtagaaga cctccggccg
     6301 ccgataccat gcgaccgggg agtcgggtct cggcgggtag agggtcgcgg tccccaccag
     6361 gtggccatcg agatagacga ccagcggaaa accccgcccc aggcgcctgg cggtgatcgt
     6421 gtcgtcctgg tgtgtggcga cgtaacgaag gccctgccgg gccagatggg cataagcgtc
     6481 gcgaaggagc gaagtcatct cggggatcga ttcgctcgag ccgtaattgc ggatgaccag
     6541 cccatccagg ctcgaagcca tcacaggcgc cgcctcggcg cctgaggaat ccggcccacc
     6601 gcccgggaac accgaacccg gctcaccgcc ccgctcggcg ccccgggacg gctccgcagg
     6661 gctcacccct tcttcggcgg ccgtagcatc agcttcacgc agcggcatgg atacccgttg
     6721 ttctctccgt cgaacgtcag ggcgggaagg aatccctcgc tctcgtagag cttccgcgcg
     6781 cgggtgttgt tctccagcgc gatcgtccag gcgtcgggcc cggccgcttc catcgcagct
     6841 cgcaacagcc ttcgcccgat cccgcggccg taatactcgg gttcgatgta cagccacgag
     6901 atgtagttgt cgcgcacgcc cgcgaaccca accgcccgat cgccatcgac cgcgacccac
     6961 ttccgagaca tccggaaatc ctcgagcgac cggaaatcct cggccagcgg gatgaatccg
     7021 cgcgggtcgc aggagccgac cagctcgtcg ggacgggaac ggtcgtggat ggcgaacaac
     7081 gccggttcgt cctcggagcg gaattcgcgg atggaagcgc tgtgggtcga ggcgtccatg
     7141 tcagctcgtc atgagaatgg gaaccgcacg ccgcagaacg agggcgacgt cgctcggcga
     7201 gctccgtacg ccgatgacga cgtccacgtc gatccccggc ccccatcgcg ggccgccgga
     7261 aacgaaatag gttcgggtgc cgtccggatc gatggggccg agggccaggg tcgcgctcca
     7321 cacatccgat ccgtgaacga cccaaagata gaccttcccc gcggtcggcg gaatcgtgtc
     7381 cggaggcgct cccttcagac ggacggtcgc gtacagcagg ctccccctga ccggatcgcc
     7441 c
//
LOCUS       DASSLZ010000113        21241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1685090, whole genome shotgun sequence.
ACCESSION   DASSLZ010000113 DASSLZ010000000
VERSION     DASSLZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 21241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21241
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685090"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1980
                     /locus_tag="VFM00_04180"
     CDS             <1..1980
                     /locus_tag="VFM00_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET7497475.1"
                     /translation="HIDDTVIFGVNMPPDGGINSNPERRGVKVMEADGIDDIGDPNSR
                     YFFGGPFDPYFVGNHTRLAPDTSPSTKTNDGAQSHVAITVTSPAGNIMNLDVNSEWRA
                     EGWPVVSEFGRSGDPPTFGSLRRDGTRQVVASSDSLIYAWMSNGEPYYPANADGQFAA
                     LPAPIVPPVLVADSLFRSNPLAAHGAAVVATAEDGLVYAFRANDRDSTASVPLAGWPP
                     SLGSGITATTAPSLGPAGDVLVGASDGTVFSIAGTDSVSFAPFVDAIADTLRIGSAPV
                     VAPAVGNLAVGRFRGPPGSWMVAYGLANGVVRLVSPVGKDPQITDIHWSVGGANFRPY
                     VLGADLDRDGETEVVVLDRAQGQVHALDLAGGELPGWPVSVIGRIEGALAAGDLDRDG
                     YPEVFAVDDQGFAHRWNRNGVEPQGWPVSLAARYGASAVGGTGSPVLGDVDGDGAPEL
                     LVATQEGLLIALEGNGTSVPGWPVASQPGPEISPLLLSLNDALTPPDPPGGPWEHLIA
                     PGGDGLWNGYQIGVRADSAYATRDGVSPRFPWTGYAGDRRHSSVLGDALLLPISATAT
                     ALARGSLYCFPNPARGADVGIAYTLGDGVGSVEIRVLDPLGNEVRKIDGPVLPSQNVA
                     RIPVRDLASGVYLVRLEVKRAGASEIAFKKFAVVR"
     gene            2111..2869
                     /locus_tag="VFM00_04185"
     CDS             2111..2869
                     /locus_tag="VFM00_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497476.1"
                     /translation="MGLASMLDFSPLPTGEILLAQRAIADTETVATPIITPSGPEAVG
                     LAPPPVSDVHKSAGRAFFYNLLLPGAGHLYAGNKRGFAHLGLEGVAWVTYFYYHGRGA
                     AKEDEYLAYADDHWSYSKWVSSCNCQGSPEDSLILYFRANNKQHYYEDIGKLSTYSGG
                     WDDAALNGSTYVSGNRNFYRGMRGDSNNLLKDARYAVMGSFLNRIVSAVDVLRMLKHR
                     SNALLGENTHIRIKLRTRPFSDQTAVGFEIRKQL"
     gene            2847..3743
                     /locus_tag="VFM00_04190"
     CDS             2847..3743
                     /locus_tag="VFM00_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497477.1"
                     /translation="MRSGNSCSAARAIPFVAAALLLTGGTSTARASWPPLALVSDTPV
                     LAKAAASGDVSASGAMTSSGSAAVSAGAAAASERLRRADQQPRKGGKKAVLLSLLLPG
                     AGEMSLGARGRATGFFIAEGAIWTHFVWFQVAGHLRKDNYIEQAQLHAGVGVSSADDT
                     YWKLLGQYQTSQGTGPTAYEEALRREARDNFPSDPAAQDAYVAERLPSGDRAWAWSST
                     DAQASYRDTRESSRRAFDRSKYSIAAAIANRLLSAIDTQIVRGRRSDEAHSAREVPGI
                     PLLLLADTAPDGSGRLYLTRSF"
     gene            3935..4756
                     /locus_tag="VFM00_04195"
     CDS             3935..4756
                     /locus_tag="VFM00_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497478.1"
                     /translation="MPRPTRLAFDADGSLYILDAGRRRLVKVGEDGTLIHDVALSGDD
                     PASTTEPADVVVDARGSALVLDRASASVLAYDKNGALLAARPLAPDLADEARDPRAAL
                     VQDSFGRLWLLAPRERDLVRLDASLARDRAGRFLTPEDSVGTPGAVAATPNGDLWLAD
                     AASGALRRFRPSGALAGRAAGDSAAPPLYAALAADGSGYLYAADALGQRIVVFDDGGS
                     RVMERALGGGEHAWRPSALAWSGRDRIAAADAARGEVQIFALERGSVRASRPRAP"
     gene            4784..8026
                     /locus_tag="VFM00_04200"
     CDS             4784..8026
                     /locus_tag="VFM00_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497479.1"
                     /translation="MSVVACAIACARGALAVAVAASGAWAPRALAAPVPTIHPGGVGA
                     PEDSIVLEFPVRRAPRLLSLRAVAPLATMAASAEPATLLRRDGDPLLSGAGAPPQDTA
                     DRQAGDALVVSGAKSFAVEMGRNRDASLHQTLDLTLRGKVAGDLEVAATLSDQRLPFE
                     PDGSTRELEDLDRVSISLRAPQGGATLGDFRLDGIPGEFARVSRALQGVQGEAKVGGL
                     RWDVAAASPKGERRSLEFRGEEGKQGPYALIGRGADSDLGGIVAGSETVWLDGVKLRR
                     GADLDYVMDYGAASLTFTVRHPVTAQSRIAVDFEGANGRYRRTFYAATTRREWGRGGW
                     YASYLREGDDAKSPLGAALTPEDRSALGAMGDSATSTLPSGVRYVGAGAGSYVWDGSD
                     PSRAHWLWLGATRGDYEVEFASVGTGRGSYADTLAADGTRFYRFMGPTLGAFEPGRAL
                     AVPRENALMDLGGAARLGALAIEGEAARSGFDRNSLSSRDDGDNAGGAGRLVARLDPR
                     AVRLFGARLGSVSAAASLRARDARFAALDRTEGAFENERWNQAPSSGGERRQEFSLAY
                     DPVAALGLRGEAGLRSLSGGSRSARRAAVLEVRSFLPGAVRWDEAKNRGPQGDGRRSK
                     WSMDFARATGPLQPRLSAALERIAGQEGDSAAARSSRLWSAGLGWNPGAALRLRSSVE
                     WRRDEARILGVSNGTEARTWEGGVAARAGDAFLADASFSRRRVAGSNGPSAADLAQLV
                     LAGGRAGGPVSSELRYDVTQLREPAVTRSLVLVGSGAGSYDAYGNAQQGGGYELVSGT
                     GEPATRSRATVQFRLDAYPGRSARAAKSALRPFGASTFFRLETLSSLPLGRLETIANP
                     SDYLASGTTLRGTLAARQSLEYVPQGSRFEARLEVGTTRDVSGELQGLESRGHGMDGR
                     LRVKRALPLGLRATATADLARNEQAVAREDGAGVYASLVRGRGYELELARAIGAAWTT
                     SLVSRHRRDVDMTRGGYQDAWSVGPSARCAGARLRLDGTATYGRLDQHGTYAPAGRYL
                     IAPLGPRVDVDLLGEYRAGDRISLSLGWNGSRVEGRPTTYTGRFELRSTF"
     gene            8026..9888
                     /locus_tag="VFM00_04205"
     CDS             8026..9888
                     /locus_tag="VFM00_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET7497480.1"
                     /translation="MTRAPFTIIAAWIALLLALPAAARASSDAGPAHPSTEYRLRAEA
                     LPESLRAVIGTGGADSTRRWSGPDLEAAALRLRDFLAGKGDVAATVRLSLTHGEGSAP
                     GTARFEIARPDPNITAAPPEGPAILAAPGAAAGPMVRVDGPLAPALADSAAAAYRAAS
                     RGAASIDGVAAGLSAARDVLIEAGFYSAEVSLDSLELGAEVPRAHLLLVPGRAATYEG
                     MELPGATATRPAAAARVAGLDKGARLTPAVLGIARERLASSGLFQEVGIPRLVTGALP
                     GGARVVIPVVEERSSRFEGALGVAREGGVTGLLDLALGNIAGSGRSAGLRWFGPGNGR
                     SEYAAHYREPALFGSRADASLALEAQVADSLFTQTRWSAEIGVAPAGAARAALALQRS
                     GSTYSGLARGSSSTWSLAARGSLDRLRPLLNPIRGFRAALMAEAGRRSDRYPGLPSES
                     RGLFRGNASAEGALALAPRRVLAASARAQGAFLSGGDFPAEELSFVGGTEGLRGHPDR
                     AFGGNRITAFSLEHRWITDERGGRLYLFADAARHEFSGRLAAGTAALSPSVESGATAA
                     STLARTVLSPGWEFGYGAGLRTRMASGLVGLELGLAPGEPLGRATIHVRYASTW"
     gene            9944..11968
                     /gene="ligA"
                     /locus_tag="VFM00_04210"
     CDS             9944..11968
                     /gene="ligA"
                     /locus_tag="VFM00_04210"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET7497481.1"
                     /translation="MASGVTAASKRAARLRKEIEEHDHRYYVLGEPVVSDQVYDALLR
                     ELAELEDQHPEIQTPDSPTRRVARGLLSGFPTVRHKVPMLSLDNTYSLDELEEFDARV
                     RKLLRVDEVEYAIEPKVDGVAVALRYEEGRFALGLTRGDGEKGDDITTNLRTIRSIPL
                     RLRGARVPAELEVRGEVYMETKEFERMNRRREAAGEKTFMNPRNATTGSLKMLDTAQV
                     ALRPLRVFVYQMTDARAQGCETHLEALERLAALGFRTNPDNGKALGVAALRRYCETWA
                     AKHASLAYGADGLVVKVNSLREQERLGATAKAPRWGIAYKFGVTEAETVLETIELQVG
                     RTGVVTPVAMLTPVTLLGTVVKRATLHNFDELERKDIREGDRVVIEKGGEVIPKVVRV
                     VPGGRRRSDPFPVPRRCPVCKEPLTRDPEQAAVRCENFLCPAQVRRRLVHWGSRGALD
                     IEGLGEKTVDLLVDQELVATPVDIYDLTAKELVPLERMAEKSAGNLLAGIEASKKAPL
                     DRLIHAIGIRHVGGTVARLLAERFRSLEALAEADAETVEKIPGIGPEIASSVAAYFGS
                     AAGRKLVRELLARGVTGTPPERRAAPETGPFAGKTFVLTGALSMPREEAERRILDAGG
                     RVTGSVSKRTDVVVAGEDAGSKLEKARALRIEVWDEAAFRAALDDAGVKR"
     gene            11999..14095
                     /locus_tag="VFM00_04215"
     CDS             11999..14095
                     /locus_tag="VFM00_04215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET7497482.1"
                     /translation="MPVTTAQHARSESVLAAFARLSRDMGFERREGQLAMAARWSEAL
                     ERGGTLAVEAPTGIGKSLAYLIPAIEHRTGGSGPILVSTHTKALQDQLVRADAPLALQ
                     ATGSRLRVMALLGRASYLCRRRAQSRLAQRRLFAADEAGHRLDERTLASLEAWIAETP
                     TGVLDELPALGIHAHPALLADIASDPFVCSGSSCEASSGCFAKAARREARRADLVVVN
                     HALLLADPGLRGTLLAESGALVLDEAHHLERVAREQRGVSLGVQDLARLAARTDAKTG
                     ALRLLARALRRGPRGATAAAALAEVDRALRPVLVSAAEMARDLESMLPPGVTAALWPR
                     GVDLARVSPVAVDRCLAAMEELIRRLEAAVAAAEADERDALRPGPTADDALDETRGRL
                     LRWVEADQAFRAVLRLEDRDVASYLDRDERGGARLNRRPLEVGSALRDSIFALSERTL
                     LTSATLAPTSDVAAAAASLGLPADEVLAIRLPSPFPLERQMATLALDGPEPNDAGFTR
                     ALAALVTRLATTLRRNTLVLLTSYQMLEDVAARVRPALEDAGIPLYAQVPGEAAGPLA
                     RAFRSGSGAVLLGTASFWEGVDFPGEALEVLVIARLPFAVPTDPVVAARSERIVESGG
                     DPFHDLALPEALLRFRQGIGRLIRTAEDRGAVVIADPRIVRSWYGERFQAVLPMPPRV
                     VRSAEEAATLLERWFS"
     gene            14092..15648
                     /gene="hutH"
                     /locus_tag="VFM00_04220"
     CDS             14092..15648
                     /gene="hutH"
                     /locus_tag="VFM00_04220"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET7497483.1"
                     /translation="MSPARTEAPAKAPARVLVGGAPLGLADLRAIAAGASVALAPGVA
                     ARVAPSRALIDDALRQHRAVYGVNTGFGALQSERVSDADLERLQLNLLRSHAAGYGEE
                     LEPAAVRLMLALRAHSLAQGMSGVRFELIERLAALLAQGILPVVPSRGSLGASGDLIP
                     LAHLALVLVGEGEARREGRAAPAAEHLRAAGLVPLTLQAKEGLALINGTQASAALGAL
                     ALAESFETLRAAHAACALSVDAMRASVKPFGEEVQRARPHAGQAASAAAVRRLLQGSG
                     ILASHADCARVQDPYSLRCAPQVMGASIQAVRYARDVLVTEVNSVTDNPLVDPESGTI
                     ISAGNFHGQPLAQALDFLAIGMAEAGAIAERRTFLLLDAAKSGLPPFLSRAAGLTSGL
                     MIVQYLQAALVSENKMLAQPASVDSIPTSAGQEDHVSMAMHAAVKGLALVRNVRRIVA
                     AELLCAAQGIDLLRPLRSSAPLEALHAGVRARVAPLTDDRRQDADLSALDAWIADGGA
                     AEAAGVESFE"
     gene            15697..17406
                     /gene="hutU"
                     /locus_tag="VFM00_04225"
     CDS             15697..17406
                     /gene="hutU"
                     /locus_tag="VFM00_04225"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET7497484.1"
                     /translation="MNPTNVQLPADPLRGTPPDEPVRAPRGTTRSCQGWHQEAALRML
                     MNNLDPEVAERPQDLVVYGGTGRAARSWPAFHAIVRSLRSLADDETLLVQSGKPVGIV
                     KTHPEAPRVLIANSLLVPKWADWDTFRELEAQGLTMYGQMTAGSWIYIGTQGILQGTF
                     ETFAEAARQRFGGSLLGRWTLTAGLGGMGGAQPLAVTMNEGICLAVEVDPERVDRRLQ
                     TRYLDRATASIDEALRWVEEAVRSKSALSIGLVGNAADVLPALVARGARPDLVTDQTS
                     AHDELNGYVPGGMALADALRLRAEKPKEYVARSVESMGRHVRAMRAFQDAGAVTFDYG
                     NNLRAQAVKAGVADAFQIPGFVPEFIRPLFCRGKGPFRWVALSGNPRDIAATDQAVLE
                     TFPEDESLRRWIRLASERVQFQGLPARICWLGMGERERFGAALHRLVERGLVEAPIVI
                     GRDHLDCGSVASPYRETEAMKDGSDAIADWPILNALVNAVSGASWVSVHHGGGVGIGN
                     SIHAGVVMVADGTPSGLARLRRVLTNDPAMGVFRHADAGYPEALETAKKNRLSIPGES
                     RAS"
     gene            17403..18680
                     /gene="hutI"
                     /locus_tag="VFM00_04230"
     CDS             17403..18680
                     /gene="hutI"
                     /locus_tag="VFM00_04230"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HET7497485.1"
                     /translation="MRSDLLVLGAAELLTAPEGTGPLRGVDLDRPLLLHDAAIACVDG
                     RIAAFGPTADVTARYPEREAAEVLDAAGCLVAPGFVDAHTHLPFAGTRENEFDARARG
                     ERYEAIAAKGGGIRASARQLRACSEERLAGLVAGRLQGLLAEGTTTVEAKSGYGLATG
                     EERKQLRALARAAASDGVEIVPTFLGAHDVPDDYRDRRGDYVALLVEEMIPAVAAEGL
                     ARFCDVWCDRGVFTVEESRRILTAGRERGLLPKLHADELGDAGGAALAAELSAVSADH
                     LLYASDDGLRAMAGAGVVAVLLPGTALTLGLPYARARDMIAMGVAVALATDFNPGSTF
                     CSSMPLMLTLGVTQMRLTPAEAWMAATANAAEAVAEGRRLGRIAPGWQADFVLYDAPD
                     YRHIPYHYGRNHVRATVKRGRVVHRRADPSICH"
     gene            18746..20287
                     /gene="ftcD"
                     /locus_tag="VFM00_04235"
     CDS             18746..20287
                     /gene="ftcD"
                     /locus_tag="VFM00_04235"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HET7497486.1"
                     /translation="MSELVECVPNFSEGRNKSVVTAIAAEISRDRSIRLLDQEMNADH
                     NRSVITFVGEAEPVLEAALRGVKKAAELIDMRHHRGEHPRMGATDVLPFVPVGTALLE
                     GCVQLARRAGERIGSELGIPVFLYEAAATRPERQNLADVRRGEYEGLARAIGTDPDRA
                     PDFGPAAIHPSAGATAVGARLPLLAFNVNLGTSDVEIAKRIAKAIRFQTGGLRYVKAL
                     GFALAERGIVQVSMNLVNTWGTPVHRVFALIRDEAERYGVPIVGSEVVGLVCQDALVD
                     VAEHALRLESFSRDQILENRLVRRIGAAEQALPEFVDEVASSAPTPGGGTVSAVAGAL
                     SGALATMVARLTIGKKKYAEVEGAMREVEREGEAIRRELLDLAAADARAFEGYRAAMK
                     LSQRTPEEVAARTRAIEAAALTAAEVPLHTAEACVRALELARTAAESGNENAVSDAGV
                     AAWLARAGAEGAALNVKINLPSVTESQRAALLSRVNGCVERARSLHHECVERVQRRMP
                     APAAV"
     gene            20309..>21241
                     /locus_tag="VFM00_04240"
     CDS             20309..>21241
                     /locus_tag="VFM00_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497487.1"
                     /translation="MDRNVAIKKKAQQLIQKGQIDAALAEFDKLFAQGDKDPYDFILV
                     ADLLAKRGSMQEAVRRYRQAVEEYTRAELFKNAIAVCKKILRISKEDLQIHRTLGELY
                     AKEGLYGDAIIHYLEFAEGSIRRQDHEAALDVIETVLALSPENDELSEKFVEIAVRAD
                     QPERGGKELYRRADRLARGGRAAEAVELRERASTLAPGSAPEPAPAADDGPPSLADGS
                     FSATPATPVPAEAARAAPQEAPSPPAFGSAGFDLDQIARAGEGEDARVPEPGVVSLHR
                     EEPPLPPAADKADAVGTHAELAAGYLSAGNRELAA"
BASE COUNT         2803 a         7634 c         7852 g         2952 t
ORIGIN      
        1 cacatcgacg acacggtgat cttcggcgtg aacatgccgc ccgacggcgg gatcaattcc
       61 aatcccgagc gccgcggcgt gaaggtgatg gaagcggacg ggatcgacga catcggcgat
      121 cccaactccc gctatttctt cggcggaccg ttcgacccct atttcgtggg gaaccacacg
      181 cgcctggcac ccgacaccag cccttcgacg aagacgaacg acggcgcgca gagccacgtc
      241 gcgatcacgg tgacgtcgcc ggccgggaac atcatgaacc tggacgtgaa ctccgagtgg
      301 cgcgccgagg gatggccggt cgtctccgag ttcggccgga gcggcgaccc gcccactttc
      361 gggagcctgc ggcgcgacgg aacccgccag gtcgtggcgt cctccgacag cctgatctac
      421 gcctggatgt ccaacggcga gccgtactat ccggcgaacg ccgacggcca gttcgccgca
      481 ctccccgcgc cgatcgtgcc gccggttctc gtggccgaca gcctcttccg gtcgaacccg
      541 ctcgcggcgc acggcgccgc cgtggtcgcc acggcggagg acggcctcgt ctacgccttc
      601 cgcgccaacg atcgcgactc gacggcgtcg gtcccgctcg cggggtggcc gccgtctctg
      661 gggagcggga tcaccgccac caccgcgccc tcgctcggtc ccgcgggcga cgtgctcgtg
      721 ggcgcgagcg acggaacggt cttctcgatc gcgggcaccg acagcgtctc cttcgcgccc
      781 ttcgtcgacg ccatcgccga cacgctccgg atcggatcgg cgcccgtcgt cgcccccgcc
      841 gtggggaacc ttgccgtggg acgcttccgc ggacccccgg gcagctggat ggtggcctac
      901 gggctggcca acggcgtcgt gcgcctcgtg agtccggtcg ggaaggatcc ccagatcacc
      961 gatattcact ggagcgtggg cggggcaaac ttccgcccct acgtgctggg cgccgatctg
     1021 gaccgcgacg gcgagacgga agtggtcgtg ctcgaccgcg cgcagggaca ggtccacgcg
     1081 ctggatctcg cgggagggga gcttccgggc tggccggtct cggtgatcgg gcggatcgag
     1141 ggggctctcg ccgccgggga cctggaccgc gacggctacc ccgaggtgtt cgcggtggac
     1201 gaccagggat tcgcgcatcg gtggaatcgg aacggggtcg agccgcaggg gtggccggtg
     1261 tcgctcgcgg cgcgctacgg cgcgtcggcg gtgggcggga ccggatcgcc ggtcctgggg
     1321 gacgtggacg gggacggcgc tcccgagctt ctggtcgcga cccaggaggg actcctgatc
     1381 gcgctggagg ggaacggcac gtcggtgccg ggatggcccg tcgcgagcca gcccggtcct
     1441 gagatctcgc cgctcctcct gtccctcaac gacgccctca cgccgcccga tccgcccggc
     1501 ggcccgtggg agcacctgat cgcccccggc ggcgacggcc tctggaacgg ctaccagatt
     1561 ggagtccgag ccgacagcgc ctacgcgacg cgggacggcg tctcgccgcg cttcccctgg
     1621 accggctacg cgggggaccg gcgccacagc tcggtgctgg gggatgcgct gcttctgcct
     1681 atctcggcca cagccaccgc cttggcacgg ggttcgctgt actgtttccc gaacccggct
     1741 cggggggccg acgtgggtat cgcctatacg ctgggcgacg gagtgggctc cgtcgaaatc
     1801 cgggtgctcg atcccctggg gaacgaggtc cggaagatcg acgggccggt gctgccgtcc
     1861 cagaatgtgg cccggatccc ggtgcgggat ctcgccagcg gggtatacct ggtgcgcctg
     1921 gaggtgaagc gtgcgggcgc gagcgagatc gctttcaaga agttcgcggt tgtccggtga
     1981 tgcggcccgg aggtagaata aaccgttgag ttcgcgtcct tttcggccgg ttcaaggaac
     2041 ccgtttcctg actcctcgcg ctcccctagc ggtccttgcc tgcacgctct gtctgctttt
     2101 gacgacaggg gtgggcctcg cctcgatgct cgacttcagt cctctgccca ccggcgagat
     2161 cctgttggcc cagcgggcga tcgcggacac cgagaccgtg gcgaccccca tcatcacccc
     2221 gagcggtccc gaggcggtgg ggctggctcc tccgcctgtt tccgacgtcc acaaatcggc
     2281 cgggagggcc ttcttttaca atctgctcct gcccggggcg ggacacctgt acgccggcaa
     2341 caagcgcggg ttcgcccatt tggggctgga aggcgtggcc tgggtgacct atttctatta
     2401 tcacggtcgc ggcgcggcga aagaggatga gtacctggcc tatgcggacg accactggag
     2461 ctacagcaag tgggtctcca gctgcaactg ccagggatct ccggaggaca gcctcatcct
     2521 gtacttccga gcgaacaaca agcagcacta ctacgaagac atcgggaagc tcagcaccta
     2581 ctcgggcggc tgggacgacg cggcgttgaa cggctcgacc tacgtctcgg gaaaccgcaa
     2641 cttctaccgg ggcatgcgcg gggacagcaa caatctcctg aaggacgccc gctacgccgt
     2701 gatgggaagc ttcctcaatc ggatcgtctc cgccgtcgac gtgctccgga tgctgaagca
     2761 ccggagcaac gcgctcctcg gcgagaacac gcatattcgt atcaagctgc gcacgagacc
     2821 cttctcggac cagaccgcgg tcggatttga gatccggaaa cagctgtagc gccgcgcgcg
     2881 ccatcccgtt cgtcgcggca gcgctcctgc tcacgggcgg gacgtcgacg gctcgcgcct
     2941 cgtggcctcc gctcgctctc gtcagcgaca ctccggtgct cgccaaggcg gcggcctccg
     3001 gcgacgtctc ggcttcgggc gccatgacgt cgagcggatc ggcggccgtc tcggccggcg
     3061 cggccgcggc gtcggagcgg ctccgacgcg ccgaccagca gccgcgcaag ggcggcaaga
     3121 aggcggtgct cctctccctg cttcttcccg gcgcgggaga gatgtcgctc ggcgcacgcg
     3181 gccgcgcgac ggggttcttc atcgcggagg gggcgatctg gacccatttc gtctggttcc
     3241 aggtcgcggg tcatcttcga aaggacaatt acatcgagca ggcgcagctt cacgccggcg
     3301 tcggcgtgag cagcgcggac gacacctatt ggaagctcct cggccagtat cagacctcgc
     3361 agggaaccgg gcccaccgcc tacgaagagg cgttgcggcg cgaggcgcgg gacaacttcc
     3421 cgtccgatcc ggcggcccag gacgcctacg tcgccgagcg gcttcccagc ggggaccgcg
     3481 cgtgggcgtg gagctccacg gacgcgcagg cgagctaccg ggacacgcgc gagagctcgc
     3541 gccgcgcgtt cgaccgctcg aagtattcga tcgcggcggc catcgcgaac cggctcctga
     3601 gcgccatcga cacccagatc gtccgggggc gccgctcgga cgaagcgcat tccgcgcggg
     3661 aagtccccgg catcccgctt ctcctcctcg ccgataccgc tcccgacggg agcgggcgac
     3721 tctacctcac ccgatcgttc tgacgtccgg cgccgcgggc cgcctcgccg cggcgatcgc
     3781 cgccgcgacg ctgatcgcgg cggggccggc gctgctcgat gtccctgccg cgcgcgccgt
     3841 cgcggcgccc gcgatccgcc aggccgccag cggccctcct gaagccctcc gcacggaacg
     3901 gatcctcgac cgcgccacgc tcgagtcgtg gggcgtgccg cgcccgacgc ggcttgcctt
     3961 cgacgccgac gggtcgctct acatcctcga cgccggacgg cggcggctgg tcaaggtggg
     4021 ggaggacggc accctgatcc acgacgtcgc gctgagcggc gacgacccgg cgtccacgac
     4081 cgagcccgcc gacgtcgtgg tggacgcacg cggatcggcg ctggtgctgg accgcgcctc
     4141 ggcgtcggtc ctcgcctatg acaagaacgg cgcccttctc gcggcgcggc ccctcgcccc
     4201 cgatctcgcg gacgaagcgc gcgatccgcg tgcggctctg gtccaggact cgttcggaag
     4261 gctgtggctc ctcgccccgc gcgagcgcga cctggtccgg ctggacgctt cgctcgcgcg
     4321 cgaccgcgcg gggcgcttcc tgacccccga ggattccgtg ggcactccgg gcgcggtcgc
     4381 cgccacgccg aacggagacc tgtggctggc cgacgccgcc tcgggcgcgc tccgccgctt
     4441 ccgcccgagc ggggccctgg cgggccgcgc cgcgggcgat tcggcggcac cgcctctcta
     4501 tgccgcgctc gccgcggacg gctcgggata cctctacgcg gccgacgcct tggggcagcg
     4561 gatcgtggtc ttcgacgacg ggggatcgcg cgtcatggag cgcgcgctgg gcggcggcga
     4621 gcacgcctgg cggccgtcgg ccctcgcctg gagcgggcgc gaccgtatcg ccgccgcgga
     4681 cgccgcccgg ggcgaggtgc agatcttcgc gctcgagcgc ggctccgtcc gcgcctccag
     4741 accgagggct ccctaggtgc gcgcgcgacg gggacgaggc gccgtgtccg tcgtcgcatg
     4801 cgcgatcgcg tgcgcgcgcg gcgccctggc cgtcgccgtc gccgcctccg gtgcctgggc
     4861 gccgcgcgcc cttgccgctc ccgttcccac gatccatccg gggggcgtcg gcgctcccga
     4921 agacagcatc gtcctcgaat tcccggtccg ccgcgcgccc agactcctct cccttcgcgc
     4981 ggtggccccg ctcgccacca tggcggcgag cgccgagccc gccacgctgc tccgccggga
     5041 cggcgatccg ctcctgtccg gcgcgggcgc cccgcctcag gacacggccg accggcaggc
     5101 cggggacgcg ctcgtcgtga gcggagccaa gtcgttcgcc gtggagatgg ggcggaatcg
     5161 cgacgcgtcc ctccaccaga cgctggacct cacgctgcgc ggcaaggtgg ccggcgacct
     5221 ggaggtggcc gcgacgctga gcgatcagcg gcttccgttc gagcccgacg gttcgacgcg
     5281 cgagctggag gatctcgatc gcgtctccat ctcgctccgc gcgccgcagg gaggcgccac
     5341 gctcggcgac ttccgcctgg acgggattcc gggcgagttc gcgcgcgtct cgcgcgcgct
     5401 gcagggcgtg cagggagagg ctaaggtggg cggcctccgc tgggacgtcg ccgccgcgag
     5461 cccgaaaggg gagcggcgct cgctggagtt ccgcggcgag gaagggaagc agggccccta
     5521 cgcgctcatc ggacgcggcg ccgactccga cctgggcggc atcgtcgccg gatcggagac
     5581 ggtctggctg gacggcgtga agctgcgccg aggcgccgat ctcgactacg tgatggacta
     5641 cggcgcggct tcgctcacgt tcaccgtccg ccatcccgtc accgcccaga gccggatcgc
     5701 cgtcgacttc gagggagcga acggccgcta ccggcgcacc ttctacgccg ccacgacgcg
     5761 ccgggagtgg ggccgcggcg ggtggtacgc ctcctacctg cgcgaggggg acgacgcgaa
     5821 gagcccgttg ggcgccgcgc tcacgccgga ggaccggagc gccctgggcg cgatgggcga
     5881 ctcggccacg agcacccttc cgagcggcgt gcgctacgtc ggcgcgggcg cgggctcgta
     5941 cgtgtgggac ggctccgatc ccagccgcgc gcactggctc tggctgggcg ccacccgcgg
     6001 ggactacgag gtggaattcg cttcggtcgg caccgggcgc ggaagctacg ccgacacgct
     6061 ggccgccgac ggcacgcgct tctaccgctt catgggtccc acgctcggcg ccttcgagcc
     6121 cggacgcgcg ctcgccgtgc cgcgcgagaa cgcgctcatg gacctggggg gcgccgcccg
     6181 gctcggagcc ctcgccatcg aaggcgaggc cgcgcgctcg ggattcgacc ggaactccct
     6241 ttcctcgcgc gacgacggcg acaacgcggg cggtgcggga aggctggtcg cccgcctcga
     6301 tccgcgcgcg gtccggctct tcggcgcccg cctcggctcg gtcagcgccg ccgcttcctt
     6361 gcgcgcgcgc gacgcgcgct tcgccgcgct cgaccgcacc gagggcgcgt tcgagaacga
     6421 gcgctggaat caggcgccct cctcgggagg tgagcgccgc caggaattct cgctcgccta
     6481 cgatccggtc gcggccctcg gcctccgcgg cgaggcgggg ctccgctcgc tgagcggagg
     6541 atcgcgttcc gcgcgccgcg cggcggtgct cgaggtgcgg tccttccttc cgggcgcggt
     6601 gcgatgggac gaggcgaaga atcgcggtcc ccagggcgac ggccggcgct cgaagtggtc
     6661 gatggatttc gcgcgcgcca cggggccgct gcagccgcgg ctctccgccg ccttggagcg
     6721 catcgccggc caggaagggg acagcgccgc cgcgcggtcc agccggctct ggagcgcggg
     6781 gctcgggtgg aacccgggcg cggcccttcg gcttcgttcc tcggtcgaat ggcggcgcga
     6841 cgaggcacgg atcctcggcg tttcgaacgg aaccgaggcg cgcacctggg agggcggcgt
     6901 cgcggcgcgc gcgggcgacg cgttcctggc cgacgcttcc ttctcgcggc ggcgcgtcgc
     6961 cggatcgaac gggccgagcg ccgccgatct ggctcagctg gtcctggccg gcggccgcgc
     7021 cggcgggccg gtgagctccg agctgcgcta cgacgtgacg cagctccgcg agcccgcggt
     7081 gacgcggtcg ctggtgctcg tgggcagtgg ggccggctcg tacgacgcgt acgggaacgc
     7141 gcagcagggc ggcggctacg agctggtgtc cggcaccggc gagcccgcca cgcgctcgcg
     7201 ggccaccgtg cagttccggc tggacgccta tcccggccgg agcgcgcgcg cggccaagag
     7261 cgcgctccga cccttcgggg ccagcacctt cttccgcctg gagaccctct cctcgctgcc
     7321 gctgggccgc ctggagacga tcgcgaatcc ttccgactat ctcgcctccg gcaccaccct
     7381 gcgagggacg ctcgccgcgc ggcagagcct cgagtacgtc ccccagggct cgcgcttcga
     7441 ggcgcgcctg gaggtgggga cgacccgcga cgtgagcggc gagctccagg gattggagag
     7501 ccgcgggcac ggcatggacg gccgcctgcg ggtgaagcgc gcccttccgc tggggctccg
     7561 cgccacggcc accgccgacc tcgcgcgcaa cgagcaggcg gtcgcgcgcg aggacggcgc
     7621 gggcgtctac gcgtcgctcg tgcgcggccg tggctacgag ctggagctgg cgcgggcgat
     7681 cggcgcggcc tggaccacgt cgctcgtctc ccgccaccgg cgcgacgtgg acatgacccg
     7741 gggcggctac caggacgcct ggtcggtggg tccctcggcg cgctgcgcgg gggcgcggct
     7801 gcgattggat gggaccgcca cgtacggccg cctggaccag catgggacct acgcgccggc
     7861 cggccgctac ctgatcgcgc cgctcggccc gcgcgtggac gtggatctcc tgggcgagta
     7921 ccgcgcgggg gaccggatct ccctgtcgct cggatggaac ggatcgcgcg tcgaaggccg
     7981 tcccacgacg tacacgggac gcttcgagct gaggagcacg ttctgatgac gcgggctccg
     8041 ttcacgatca tcgccgcttg gatcgcgctg ctcttggcgc tcccggccgc cgcgcgcgcc
     8101 tcctcggacg cgggtcccgc gcatccttca accgaatacc ggctgcgcgc ggaagcgctc
     8161 cccgaatcgc ttcgcgccgt gatcgggacc ggcggtgccg attccacccg gcgctggagc
     8221 ggtcccgacc tggaggcggc cgcgctccgc ctccgcgatt tcctggcggg aaaaggggac
     8281 gtcgcggcca cggtgcggct ttcgctcacg catggcgagg ggagcgcgcc cggaacggcg
     8341 cgcttcgaga tcgcgcgccc cgatccaaac atcaccgccg cccctcctga agggccggcg
     8401 atcctcgccg cgcccggagc cgccgcggga cccatggtcc gcgtcgacgg cccgctcgct
     8461 cccgccctcg ccgactcggc ggccgccgcg taccgtgccg ccagccgcgg cgctgcttcc
     8521 atcgacggcg tggccgcggg gctctcggcc gcgcgggacg tgctgatcga agcgggcttc
     8581 tattcggcgg aggtctcgct cgactcgctg gagctgggcg cggaggttcc gcgcgcgcac
     8641 ctgctcctgg tgcccgggcg ggccgcgacg tacgaaggga tggagcttcc cggggctacg
     8701 gccacgcgtc ccgcggccgc cgcgcgcgtc gcggggctcg acaagggcgc gcgcctcacg
     8761 ccggccgtct tgggcatcgc gcgcgagcgt cttgcctcgt cgggcctctt ccaggaggtg
     8821 gggattccgc gcctggtgac gggagcgctc cccggaggcg cccgggtcgt gatcccggtg
     8881 gtggaagagc gctcgagccg cttcgagggc gcgctcggcg tcgcgcgcga aggaggcgtc
     8941 acggggctcc ttgatctggc gctcgggaac atcgcgggga gtggacggtc ggcgggactc
     9001 cgatggttcg gcccgggcaa cggccgctcc gagtacgccg cgcactatcg cgagcccgcg
     9061 ctcttcggaa gccgcgccga cgcctcgctc gcgctggagg cgcaggtcgc ggactcgctc
     9121 ttcacgcaga cgcggtggtc ggccgagatc ggcgtcgccc ccgcgggcgc cgcgcgcgcc
     9181 gcgctcgcgc tccagaggag cgggagcacc tattcgggtc tcgcgcgcgg gtccagctcc
     9241 acgtggtccc tggcggcgcg cggcagcctg gaccgccttc ggccgctcct gaatccgatc
     9301 cgcggattcc gcgcggcgct gatggccgag gcggggcgcc gatcggaccg ctaccccggc
     9361 ctgccgtccg agagccgcgg gctcttccgg ggcaacgcgt ccgccgaagg ggcgctcgcg
     9421 ctggcacccc gccgggtgct cgccgcctcc gcgcgcgcac agggcgcgtt tctttcgggc
     9481 ggcgatttcc cggccgagga gctctcgttc gtcgggggca ccgaggggct gcgcggtcat
     9541 cccgatcgcg cgttcggagg gaaccggatc accgcgttct cgcttgaaca tcgctggatc
     9601 accgacgagc gggggggccg tctctatctc ttcgcggatg ccgcgcgaca cgaattctcg
     9661 ggcaggcttg ccgccggaac cgcggcgctc tctccctcgg tggagtcggg cgccaccgcc
     9721 gcctcgacgc tggcccgaac ggtgttgagc ccgggctggg agttcggata cggtgccgga
     9781 ctcaggacga ggatggcgtc gggcctggtg ggcctggagc tgggtttggc accgggggag
     9841 ccgctcggga gggcgacgat ccacgtccgc tacgcgagca cctggtgatc tcgaatcgga
     9901 gggggtggat gggacggggc ggcgcgccgc ggcgggaggg gacatggcgt ccggcgtgac
     9961 cgcggcgtcg aagcgcgccg cgcggctccg caaggagatc gaggagcacg accaccgcta
    10021 ctacgtgctc ggcgagcccg tcgtctccga ccaggtctac gacgcgctcc tgcgcgagct
    10081 cgcggagctg gaggaccagc accccgagat ccagaccccc gattcgccga cgcgccgcgt
    10141 ggcgcgcggc ctcctctccg gatttcccac cgttcgccac aaggtcccga tgctcagcct
    10201 ggacaacacc tactccctcg acgagctgga ggagttcgac gcgcgcgtgc ggaagcttct
    10261 ccgcgtcgac gaggtggagt acgcgatcga gcccaaggtg gacggcgtgg ccgtcgcgct
    10321 ccgctacgag gaagggcgct tcgcgctggg actcacgcgc ggcgacgggg agaaggggga
    10381 cgacatcacc acgaatctcc ggacgatccg gtcgatcccg ctccggctgc gcggcgctcg
    10441 ggtgcccgcc gagctggagg tgcggggcga ggtctatatg gagacgaagg agttcgagcg
    10501 gatgaaccgc cggcgcgagg ccgcggggga gaagacgttc atgaatcccc gtaacgcgac
    10561 cacgggaagc ctcaagatgc tcgacaccgc gcaggtggcg ctccgtcctc tccgcgtctt
    10621 cgtctaccag atgacggatg cgcgagcgca ggggtgcgag acgcacctgg aggcgctcga
    10681 acgcctggcc gcgctcggat tccggaccaa tcccgacaac ggaaaggccc tcggcgtcgc
    10741 ggcgctccgg cgctactgcg agacctgggc ggcgaagcac gcctcgctcg cgtatggcgc
    10801 ggacgggctc gtggtcaagg tgaactcgct ccgcgagcag gagcggctcg gcgccaccgc
    10861 caaggcgccg cgctggggga tcgcctacaa gttcggcgtg accgaggcgg agaccgtgct
    10921 cgagacgatc gagctgcagg tcgggcggac gggcgtggtc acgccggtcg cgatgctcac
    10981 gcccgtcacg ctcctcggca ccgtcgtgaa gcgggcgacg ctccacaatt tcgacgagct
    11041 ggagcggaag gacatccgcg aaggggaccg cgtcgtgatc gagaaggggg gcgaggtgat
    11101 ccccaaggtg gtccgcgtcg tgccgggggg acggcggcgc tccgatccct tcccggtgcc
    11161 gcgccggtgt cccgtctgca aggagccgct gacgcgcgat ccggagcagg ccgcggtccg
    11221 ctgcgagaac ttcctctgcc cggcccaggt ccgacggcgg ctggtgcact gggggagccg
    11281 cggcgccctg gacatcgagg ggctcggcga gaagaccgtg gacctcctgg tggaccagga
    11341 gctggtcgcg acgccggtgg acatctacga cctcacggcg aaggagctgg tcccgctgga
    11401 gcggatggcg gagaagtcgg cgggcaatct cctcgccggg atcgaggcga gcaagaaggc
    11461 gccgctcgac cggctgatcc atgccatcgg catccgccac gtcggaggca cggtggcgcg
    11521 gctgttggcc gagcgcttcc gctcgctcga ggcgctcgcg gaagcggacg cggagacggt
    11581 ggagaagatc ccgggcatcg gtcccgagat cgcgagcagt gtcgccgcgt acttcgggag
    11641 cgccgcgggg cggaagctgg tccgcgagct cctcgcgcgc ggcgtgacgg gcacgccgcc
    11701 cgagcggcgc gccgcccccg agaccggccc cttcgcgggg aagaccttcg tcctgaccgg
    11761 cgccctctcc atgccgcgcg aagaggcgga gcgccgtatc ctggacgcgg gcgggcgggt
    11821 caccggttcg gtcagcaagc ggaccgacgt ggtcgtggcg ggggaggacg cggggtccaa
    11881 gctggagaag gcgcgggcgc tccggatcga agtctgggac gaggccgcct tccgcgccgc
    11941 gctcgacgac gcgggcgtca agcggtaggc tatcgccatc catgacgagt cccctcccgt
    12001 gcccgtgacc acggcccagc acgcccgctc cgaatccgtg ctggccgcgt tcgcgcgcct
    12061 gtcgcgcgac atgggattcg agcgccgcga ggggcagctc gcgatggccg cccgctggag
    12121 cgaggcgctc gagcgcgggg gcaccttggc cgtggaggcg ccgacgggga tcgggaagtc
    12181 gctcgcctat ctcatccccg ccatcgagca ccggacgggc gggtcggggc cgatcctcgt
    12241 gagcacccac accaaggcgc tccaagacca gctggtgcgc gccgacgccc cgctcgcgct
    12301 ccaggcgacc ggatcgcgcc ttcgcgtcat ggcgctcctc ggacgcgcct cctacctctg
    12361 ccgccgccgc gcccagtcga ggctggcgca gcgaaggctg ttcgccgcgg acgaggcggg
    12421 gcaccggctg gatgagcgga cgctggcctc gctcgaggcg tggatcgccg agacgccgac
    12481 cggcgtgctg gacgagctgc ccgcgctggg gatccacgcc cacccggcgc tcctggccga
    12541 catcgcctcg gatcccttcg tctgctccgg ctcctcctgc gaggcctcga gcggctgctt
    12601 cgccaaggcc gcgcgccgcg aggcccggcg cgcggacctc gtcgtcgtga accacgcgct
    12661 cctcctcgcc gacccgggcc tccgcggcac gctcctcgcc gagtcggggg cgctggtcct
    12721 ggacgaggcg catcacctcg agcgcgtcgc ccgcgagcag cgcggcgttt cgctgggcgt
    12781 gcaggacctg gcccgtctcg ccgcgcgcac cgacgccaag accggggcac tccgcctgct
    12841 ggcgcgcgcg ctccggcgcg gtccccgcgg ggccacggcc gccgcggcgc tggccgaggt
    12901 cgaccgcgcg ctccgcccgg tgctggtgag cgcggcggag atggcgcgcg atctggaatc
    12961 gatgctccct ccgggagtga ccgccgccct ctggccccgc ggcgtcgacc tcgcccgcgt
    13021 gagcccggtc gcggtggatc gctgcctcgc ggcgatggag gagctgatcc gccggctcga
    13081 ggcggccgtg gccgccgccg aggcggacga gcgggacgcg ctccggccgg ggccgacggc
    13141 ggacgacgcg ctcgacgaga cgcgcgggcg gctcttgcgc tgggtcgagg ccgaccaggc
    13201 gttccgcgcg gtcctgcgcc tggaagatcg cgacgtggcg tcctacctcg atcgcgacga
    13261 gcggggtggc gcgcgactga accggaggcc gctcgaggtg gggagcgcgc tccgcgattc
    13321 catcttcgcc ctctccgagc gcaccctgct gacgtcggcc acgctagcgc ccacgagcga
    13381 cgtggcggcc gcggccgcgt cgctgggcct gccggccgac gaggtccttg cgatccggct
    13441 cccgtctccc tttccgctcg agcgccagat ggcgacgctc gcgctggacg gacccgagcc
    13501 caacgacgcg ggcttcacgc gcgcgctcgc cgcgctggtc acgcgtctgg cgaccacgct
    13561 ccggcggaac acgctcgtcc ttctcacctc ctatcagatg ctggaggacg tggccgcgcg
    13621 ggtccggccc gcgctggagg acgccggcat tccgctgtac gcccaggttc cgggcgaggc
    13681 ggccgggccg ctcgcccgcg cgttccggag cggttctgga gcggtgctcc tcggcacggc
    13741 ctccttctgg gagggagtgg actttccggg cgaagcgctc gaggttctcg tgatcgcgcg
    13801 gctccccttc gcggtgccga ccgatccggt cgtcgccgcg cgctccgagc ggatcgtgga
    13861 gtccggcggc gacccgttcc acgatctggc gctgcccgag gcgctgctcc gcttccggca
    13921 ggggatcggc cggctcatcc gcacggccga ggaccggggc gccgtcgtga tcgccgaccc
    13981 ccgcatcgtc cgctcgtggt acggggagcg cttccaggcc gtgctcccca tgcccccgcg
    14041 cgtggtgcgg agcgcggagg aagccgcgac gctcctggaa cggtggtttt cgtgagcccg
    14101 gctcgcaccg aggcgccggc gaaggcgccg gcccgcgtcc tggtcggtgg cgcgccgctc
    14161 ggcctcgcgg accttcgcgc gatcgcggcg ggcgcttcgg tcgcgctcgc ccccggagtc
    14221 gcggcgcgcg tcgccccctc gcgcgcgctg atcgacgacg cgctgcgcca gcaccgggcg
    14281 gtctacggcg tgaacaccgg cttcggcgcg ctccagtcgg agcgcgtctc cgacgcggat
    14341 ctggagcggc tgcagctgaa cctgctccgg agccacgcgg cgggctacgg cgaagagctc
    14401 gagcccgcgg cggtgcggct catgctcgcg ctccgggccc actcgctcgc ccaggggatg
    14461 agcggggtgc gcttcgagct gatcgagcgc ctcgcggcgc tgctcgcgca ggggatcctt
    14521 ccggtcgttc cgagccgggg gtccttgggc gcgagcggcg acctgatccc gctcgcccat
    14581 ctcgcgctgg tgctcgtcgg ggagggggag gcgcgccggg aaggacgcgc cgcgcccgcg
    14641 gctgagcacc tgcgcgccgc cggtctggta cccctcacgc tccaggcgaa ggaggggctc
    14701 gccctgatca acggcacgca ggcctccgcc gcgctcggcg cgctcgccct cgccgagtcg
    14761 ttcgagaccc tgcgcgcggc gcacgccgcg tgcgccctca gcgtggacgc gatgcgcgcg
    14821 agcgtgaagc cgttcggcga ggaagtgcag cgcgcgcggc cgcacgcggg gcaggcggcg
    14881 tcggcggcgg cggtgcgccg gctgctccaa gggagcggca tcctcgcctc gcacgccgac
    14941 tgcgcgcggg tgcaggaccc ctattcgctc cgctgcgcgc cgcaggtgat gggggcctcg
    15001 atccaggcgg tgcgctacgc gcgcgacgtg ctggtcaccg aggtgaacag cgtgaccgac
    15061 aacccgctcg tcgatcccga gagcgggacc atcatctcgg cgggaaattt ccacgggcag
    15121 ccgctcgccc aggcgctcga tttcctcgcg atcggcatgg ccgaggcggg cgcgatcgcc
    15181 gagcggcgga cgttcctgct cctcgacgcg gccaagagcg gtcttccgcc gttcttgagc
    15241 cgggccgcgg ggcttacctc ggggctcatg atcgtgcagt atctgcaggc cgcgctcgtc
    15301 tccgagaaca agatgctggc gcagcccgcc agcgtggact cgattcccac ctccgcgggg
    15361 caggaagatc acgtgagcat ggccatgcac gcggcggtca aggggcttgc gctcgtgcgc
    15421 aacgtgcgcc ggatcgtggc ggccgagctt ctctgcgccg cgcagggaat cgacctcctg
    15481 cggccgcttc gttcgagcgc gccgctcgag gcgctgcacg cgggggtgcg ggcgcgggtg
    15541 gcgccgctca cggacgaccg gaggcaggac gccgatctgt cggcgctgga cgcgtggatc
    15601 gccgacggcg gcgcggcgga agcggcgggc gtggaatcct tcgaatgagg tgctccgccg
    15661 cccaaggcgg cgggagcttg gggtgaggag acaccgatga atccgacgaa cgtacagctg
    15721 cccgcggatc cgctccgggg gacgcccccc gacgagccgg tgcgcgcccc gcgcggcacc
    15781 acccgctcgt gccagggatg gcaccaggaa gccgcgctcc ggatgctcat gaataacctg
    15841 gaccccgagg tggccgagag gccgcaagac ctggtggtct acggcggcac cgggcgggcg
    15901 gcgcgctcct ggcccgcgtt ccacgccatc gtccgttcgc tccgctcgct cgccgacgac
    15961 gagacgctgc tcgtccagtc ggggaagccc gtcggcatcg tgaagaccca ccccgaggcg
    16021 ccgcgcgtcc tcatcgccaa ctcgctcctc gtccccaagt gggccgactg ggacaccttc
    16081 cgcgagctcg aggcgcaggg gctcacgatg tacgggcaga tgaccgccgg gtcctggatc
    16141 tacatcggca cgcaggggat cctccagggc actttcgaga ccttcgcgga ggcggcccgg
    16201 cagcgtttcg gcggctccct cctcgggcgc tggacgctca cggcggggct gggcggcatg
    16261 ggcggcgcgc agccgctcgc cgtcacgatg aacgagggga tctgcctcgc ggtcgaagtg
    16321 gatcccgagc gggtggaccg gcggctccag acgcgctacc tggaccgggc gaccgcctcg
    16381 atcgacgagg cgctccgctg ggtcgaggag gccgtgcgat cgaagagcgc gctctcgatc
    16441 gggctcgtgg ggaacgctgc cgacgtgttg ccggcgctgg tcgcgcgggg cgcccgcccc
    16501 gacctggtca ccgaccagac ctccgcccat gatgagctga acggatacgt cccgggcgga
    16561 atggccctgg ccgacgcgct ccgcctccgc gccgagaagc cgaaggagta cgtcgcgcgc
    16621 tcggtcgaat cgatggggcg ccacgtgcgc gccatgcgcg ccttccagga cgcgggcgcg
    16681 gtgaccttcg actacggcaa caacctccgc gcgcaggcgg tgaaggccgg cgtcgccgac
    16741 gccttccaga tcccaggctt cgtgccggag ttcatccggc cgctcttctg ccgggggaag
    16801 ggtcccttcc gctgggtcgc gctctccgga aatccccggg acatcgccgc gaccgatcag
    16861 gcggtgctcg agaccttccc cgaggacgag tcgctccgcc gctggatccg tctcgcgtcg
    16921 gagcgggtgc agttccaggg gctgccagcg cggatctgct ggctgggcat gggcgagcgc
    16981 gagcggttcg gcgcggcgct gcaccgcctg gtggagcgcg ggctggtcga ggcgccgatc
    17041 gtcatcggcc gcgaccatct cgactgcggc tcggtcgcct cgccctaccg cgagaccgag
    17101 gcgatgaagg acggcagcga cgccatcgcc gactggccga tcctcaacgc cctggtgaac
    17161 gcggtctcgg gcgcctcgtg ggtgtcggtg caccatggcg gcggcgtggg gatcgggaat
    17221 tcgatccacg ccggcgtggt catggtcgcc gacggcactc cctcgggcct ggcgcggctc
    17281 cgccgcgtgc tcacgaacga tccggcgatg ggcgtcttcc gccacgccga cgcgggctat
    17341 cccgaagcgc tggagacggc gaagaagaac cggctttcca ttcccggcga atcccgcgca
    17401 tcgtgaggag cgacctgctc gtcctcgggg cggccgagct cctgaccgct cccgagggaa
    17461 caggaccgct ccgcggggtc gatctcgacc ggccgctgct cctccacgac gcggcaatcg
    17521 cctgcgtgga cgggcgcatc gccgcgttcg gccccaccgc cgacgtgacc gcgcgctatc
    17581 ccgagcgcga ggcggccgag gtgctggacg ccgcgggctg cctcgtcgcg cccgggttcg
    17641 tggacgccca cacccatctt cccttcgcgg gcacgcgtga gaacgagttc gacgcgcgcg
    17701 cgcgcggcga gcgctacgag gcgatcgccg ccaagggggg cgggatccgc gcgagcgcgc
    17761 gccagctccg cgcgtgctcc gaagagcggc tcgccggtct ggtggccggc cggctccaag
    17821 gtctcctggc cgaggggacg accaccgtcg aggcgaagag cggctacggg ctcgccacgg
    17881 gcgaagagcg aaagcagctc cgcgccctcg cccgcgccgc cgccagcgac ggcgtggaga
    17941 tcgtcccgac cttcctgggc gcgcacgacg tgcccgacga ctatcgcgac cgccgcggtg
    18001 actacgtcgc ccttctcgtc gaggagatga tccccgccgt cgccgccgag gggctcgcgc
    18061 gcttctgcga cgtctggtgc gatcgcggcg tgttcacggt ggaggagtcg aggcgaattc
    18121 tcacggcggg gcgcgagcgt gggcttttgc ccaagctcca cgccgacgag ctgggcgacg
    18181 ccggcggcgc cgcgctcgcg gccgagctct ccgcggtctc cgccgatcac ctgctctacg
    18241 cgagcgacga cggccttcgc gccatggccg gcgcgggcgt ggtcgcggtg ctcctgccgg
    18301 gcaccgcgct caccctgggt cttccctacg cccgcgcccg cgacatgatc gccatgggcg
    18361 tggcggtcgc gctcgccacc gacttcaatc cggggagcac cttctgttcc tccatgccgc
    18421 tcatgctcac gctcggcgtg acgcagatgc gcctcacgcc ggccgaggcc tggatggcgg
    18481 ccaccgcgaa cgccgcggag gcggtcgccg aggggcggag gctggggcgg atcgcccccg
    18541 ggtggcaggc cgatttcgtc ctctacgacg cccccgatta ccggcatatt ccttaccact
    18601 acggccggaa ccacgtgcgg gccaccgtga agcggggaag ggtggtccat cgccgcgccg
    18661 acccttcgat ctgccattaa gaaaatcgag taagaggcgg ccattgggcc gcttggatcc
    18721 cccaagctaa cgaccaggag aaggcatgag cgagctcgtg gaatgcgtcc ccaacttcag
    18781 cgaaggccga aataaatctg tagttacggc cattgctgcc gaaatttcgc gtgatcggtc
    18841 gatccgcctg ctcgaccagg agatgaacgc cgaccacaac cgttcggtga tcacgttcgt
    18901 gggcgaagcg gagcccgtgc tggaagcggc gctccgaggc gtgaagaagg cggccgagtt
    18961 gatcgacatg cgccatcacc ggggggagca tccacggatg ggagccaccg acgtcctgcc
    19021 attcgtcccg gtcggcaccg ctctgctcga gggatgcgtc cagctcgcgc ggcgcgccgg
    19081 cgagcggatc ggcagcgagc tcgggatccc ggtttttctg tatgaagcgg ccgcgacgcg
    19141 gcccgaacgc cagaacctgg cggacgtgcg gcgcggcgaa tacgaagggc tagcccgggc
    19201 gatcgggacg gatccggatc gggcgcccga cttcgggccc gccgcgatcc atccctcggc
    19261 gggcgcgacg gcggtcggcg cgcgcctgcc gctcctcgcc ttcaacgtga acctcggcac
    19321 gtcggacgtc gagatcgcga agcggatcgc caaggcgatc cggttccaga ccggcgggct
    19381 gcgctacgtg aaggcgctgg ggttcgcgct ggcggagcgg gggatcgttc aggtctcgat
    19441 gaacctcgtg aacacgtggg gcactccggt gcaccgggtg ttcgcgctca tccgggacga
    19501 ggcggagcgg tacggcgttc cgatcgtcgg aagcgaggtg gtggggctgg tctgccagga
    19561 cgcgctcgtg gacgtggcgg agcacgcgct ccgtctggag agcttctcgc gcgaccagat
    19621 cctggaaaac cgtctcgtgc gccggatcgg cgcggcggag caggcgctgc ccgagttcgt
    19681 ggacgaggtg gcttcgtccg cgccgacgcc gggcggcggc acggtctcgg ccgtggccgg
    19741 cgccctctcg ggcgcgctcg cgacgatggt cgcgcggctc acgatcggga agaagaagta
    19801 cgcggaggtg gagggggcga tgcgcgaggt cgagcgtgaa ggcgaggcga tccgccgcga
    19861 gctcctggac ctcgcggcgg ccgacgcgcg cgccttcgag ggctatcgcg cggcgatgaa
    19921 gctctcgcag cggacgccgg aggaggtcgc ggcgcgaacc cgcgcgatcg aggccgcggc
    19981 gctcacggcg gccgaggtgc cgctccacac cgccgaagcg tgcgtgcgcg cgctggagct
    20041 ggcccgcacg gcggccgagt cggggaacga gaacgcggtc agcgacgcgg gggtggcggc
    20101 ctggctcgcc cgggcgggag ccgagggcgc cgcgctcaac gtgaagatca acctgccgtc
    20161 ggtgacggaa tcgcaacgcg cggcgcttct ttcgcgcgtg aatggctgcg tggagcgggc
    20221 tcgctcgctc caccacgagt gcgtcgagcg cgtgcaacgg cgaatgccgg cgcccgcggc
    20281 cgtctaggac gaggtcgggg aaccgggcgt ggaccgaaac gtcgcgatca agaagaaggc
    20341 gcagcagctc atccagaagg ggcagatcga cgccgctctg gccgagttcg acaagctgtt
    20401 cgcgcagggg gacaaggacc cctacgactt catcctggtc gccgacctcc tggccaaacg
    20461 cggatcgatg caggaagcgg tgcgccgcta ccggcaggcc gtcgaggagt acacccgggc
    20521 cgagctcttc aagaacgcga tcgccgtctg caagaagatc ctccggattt ccaaggaaga
    20581 cctccagatc catcgcaccc tcggcgagct gtatgccaag gagggactct acggcgacgc
    20641 gatcatccat tatctcgaat ttgccgaggg ctcgatccgc cgccaggatc acgaggccgc
    20701 gctcgacgtg atcgagacgg tcctggcgct gtcccccgag aacgacgagc tctccgagaa
    20761 gttcgtcgag atcgcggtgc gcgccgacca gcccgagcgc ggcgggaagg agctctatcg
    20821 ccgcgccgac cgtctcgcgc gcggggggcg cgcggccgag gcggtcgagc tgcgcgagcg
    20881 cgcctccacg ctcgcgcccg gatccgcgcc cgagccggcg cccgcggcgg acgacggtcc
    20941 gccgtcgctg gccgacggca gcttctcggc cacgcccgcc acgccggtcc cggccgaggc
    21001 ggcgcgcgcg gcgccgcagg aagcgccgag cccgcccgcc ttcggatcgg ccgggttcga
    21061 tctggatcag atcgcgcgcg cgggcgaggg cgaggacgcg cgcgtgccgg agccgggcgt
    21121 ggtctcgctg caccgcgagg agccgcccct tccacccgca gcggacaagg cggacgccgt
    21181 gggcacgcac gccgagctgg ccgccggcta cctgagcgcg ggcaaccgcg agctggcggc
    21241 g
//
LOCUS       DASSLZ010000114         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_39018, whole genome shotgun sequence.
ACCESSION   DASSLZ010000114 DASSLZ010000000
VERSION     DASSLZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39018"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VFM00_04245"
     CDS             <1..1177
                     /locus_tag="VFM00_04245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7497488.1"
                     /translation="GEPLARASRPHWHARSRLLYRSLSHLISVERKLFTRDERNAEYL
                     QTDAGDILRTILASARQILECDDARLYPLTRTLDEPRYMEPVSEGEPWNRALAEAWVG
                     RRGLPLYVADVAEGGLVHAMVDAEAMDATMHLAVAPFSARPEPSGLRSVAMARVGEPG
                     SPLLYVLEAWSSEPGFFTDERVGILGVVAEHATDLLNTLKKLGMLVMIDELTGVYNRP
                     YFGRQLDHEIARAYREGRPMSLVIADIDDFKNVNDTYGYEAGNIVLRELSQTLSSSLR
                     PFDTVARWGGEEFAIILSAETTRGEAREICERLRLKVQSMAILVPGLDGRENSVRLTM
                     SLGGSMFPSDVPMSADRSRGLDRPARDKIGHDLWTRANMNLRVAKERGKNQVTYSGE"
     gene            1532..>2324
                     /locus_tag="VFM00_04250"
     CDS             1532..>2324
                     /locus_tag="VFM00_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497489.1"
                     /translation="MRFRFAIDSDTLKPALKWHVTYGHDSVFTFHVAPGDLVGGHVFW
                     VSAEDNDGKIDHTPAKRYFSIRTVPPTSQITGGPASGAVAGPTTTFSWSGNDPDGSET
                     GGPVPVDSFEYMLLRPGTSFDGNHTPLPVSLSPTMLALLNEASGNSLPAPYDDWKWTA
                     TSARSHTFTGLDPASYVFAVRAVDAAGAHSVLQAPRNVRGFWVTPSPERQSAPWMRIT
                     SEALTLPITSDQSPIDGPSIRQRFSILDGEPVSFSWVGLPSLAGYP"
BASE COUNT          373 a          810 c          752 g          389 t
ORIGIN      
        1 cggcgagcct ctggcgcgcg cgtcccgtcc ccactggcat gcgcggagcc gccttctgta
       61 ccggagcctc tcccacctga tctcggtgga gcggaagctc ttcacgcgcg acgagcgtaa
      121 cgctgaatac ctccagaccg acgctggcga catcctccgc accattctcg cttcggcccg
      181 gcagatcttg gagtgcgacg acgcgcgcct ctatcccctg acccgcacgc tggacgagcc
      241 gcgatacatg gagccggtga gcgagggaga gccgtggaat cgagcgctgg ccgaggcgtg
      301 ggtcggccgg cgcgggctcc ccttgtacgt ggccgacgtg gcggagggtg gactcgtgca
      361 cgccatggtg gatgccgagg cgatggacgc gacgatgcac ctggcggtgg cacccttctc
      421 cgcgcgcccc gagcccagcg ggctccgctc ggtggcgatg gcgcgcgtgg gcgagccggg
      481 gtcgccgctc ttgtacgtgc tcgaggcgtg gagctccgag cccggcttct tcaccgacga
      541 gcgcgtcggg atcctgggcg tggtggccga gcacgcgacc gatctcctga acacgctgaa
      601 gaagctgggc atgctggtca tgatcgacga gctgaccggc gtctacaacc gcccctactt
      661 cgggcgccag ctcgatcacg agatcgcgcg cgcctaccgc gaggggcgcc cgatgtccct
      721 cgtcatcgcc gacatcgacg acttcaagaa cgtgaacgac acgtatggat acgaggccgg
      781 caacatcgtg ctgcgcgagc tgtcgcagac tttgtcctcc agcctgcgcc cgttcgacac
      841 cgtcgcgcgc tggggcggcg aggagttcgc catcatcctg agcgccgaga cgacgcgcgg
      901 cgaagcgcgc gagatctgcg agcggctccg gctgaaggtg cagagcatgg ccatcctggt
      961 gcctgggttg gacggccgcg agaactccgt ccgcctgacg atgagcctcg gcggctcgat
     1021 gttcccgagc gacgtcccga tgagcgccga ccgctcgcgc gggctcgatc gccccgcacg
     1081 cgacaagatc ggccacgact tgtggacccg cgcgaacatg aatctcaggg tggcgaagga
     1141 gcgcggaaag aatcaggtga cgtacagcgg ggagtaggtt ttggcgccgc ggccctgaca
     1201 atgtggcagg gcccctaagt acccctggcg ccgcgctggg agcaggacta tcgtgacctg
     1261 agaggtttca tcccccacgg caactcacgg gcgcatcccc cgcgccgctc gccggcatcg
     1321 gacccggacc atgtgtcgct ggcgtcgaat cgctccggtc ctcttcgccc tggccctttc
     1381 ataccttctc ggctgctcca agaacccgac acggctcatc atcgaacgag gcgcgcccga
     1441 aacggagctg acctacgctc ccgtcgaggc ggacaccgcc tcgttccgcg ttcatctcta
     1501 ctggaacggc tacgacagcg acggcgaggt ggtgcggttc cgcttcgcga tcgattccga
     1561 caccttgaag cccgccctga agtggcacgt gacctacgga cacgattccg tgttcacgtt
     1621 ccatgtggcg ccgggcgacc tcgtgggcgg tcacgtgttc tgggtttccg ccgaggacaa
     1681 tgacggaaag atcgaccaca cacccgccaa gcggtacttc tcgattcgaa ccgtgccgcc
     1741 cacctcgcag atcacaggcg ggccggcgtc tggagcggtg gccggcccga ccacaacctt
     1801 ctcctggagc ggcaacgatc cggatggcag tgaaacgggt ggccccgttc ccgtggactc
     1861 gttcgagtac atgctgctcc ggccggggac ttcgtttgac ggcaatcaca cgcccctgcc
     1921 tgtctcgcta tctccgacta tgctcgccct gctcaatgag gcctcgggca actccttgcc
     1981 ggcgccctac gacgattgga agtggacggc aacgtccgcg cgttcgcaca cgttcaccgg
     2041 cctggacccc gcctcctatg tgttcgcggt tcgcgcggta gacgccgcag gagcgcacag
     2101 cgtgcttcag gccccccgca acgtccgggg cttctgggtc accccatcac cggaacgaca
     2161 gtcggcgcca tggatgcgca tcacaagcga agccctgacc cttccgatca cgagcgacca
     2221 gtcccccatc gatggtcctt ccatccggca gcggttctcg atcctggacg gggagcccgt
     2281 gtcgttctcc tgggtgggcc tgccgagtct cgccgggtac ccgc
//
LOCUS       DASSLZ010000115         7649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1685434, whole genome shotgun sequence.
ACCESSION   DASSLZ010000115 DASSLZ010000000
VERSION     DASSLZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685434"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFM00_04255"
     CDS             <1..340
                     /locus_tag="VFM00_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497490.1"
                     /translation="LGMGTPQGTTIPIVDSTGVVVGKRRDPEGKVVQTKLDEALLRDL
                     AKRGGGTYERGDGTGRAALHLADAMRSEGGVEARGQTVRAYDERFAWFAAGAGLLLVL
                     ERLVPRRRKR"
     gene            337..1032
                     /locus_tag="VFM00_04260"
     CDS             337..1032
                     /locus_tag="VFM00_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497491.1"
                     /translation="MKRALLLVLLAASTLGAFYEWGGPARRGVRQLKKGEDAAALHSF
                     GEARPEQPTSAAVRYDQGIAFRKLGLTDSSSTAFRDAMKLQGTAARSAAGYNLGNEAL
                     RKNQLDEAIEGYKESLRADPRRADAKKNLEEALRRLREAKPPPTPPSKGGSGSKGPSG
                     PGSGGGGGGGNPQPNAPPSLGQQNTPPSASGPIPSRSDAELWLDALESERRSERQREK
                     QQSEPRRENVRDW"
     gene            1022..2794
                     /locus_tag="VFM00_04265"
     CDS             1022..2794
                     /locus_tag="VFM00_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HET7497492.1"
                     /translation="MTGSARVRFAAVALLLAVSAPARAAAPRLSIEAQLDKDHVAVGE
                     EAQITVVAEARGVNLPDYALPSVPGLHIVRVANSQNFSWMNGNLSRSTTSIFLVSAAA
                     PGRYTIPPVAIVSGRERAQTRPIVLDVGGATAQAPGAPGAPPGEAIPPRVWGDESLPE
                     MFVRLVVDRRRAYWNQQITARFVLFARDRLEEVPMWEVAEANGFWKESLGEMKRGRVR
                     VGKEDYIAYEQDVAYFPTRTGKLTLGPGRIEARVARATTAPDPWSLLGFPETQVETIP
                     LQTESATITVLPLPEGAPPGFRGAVGRLSMDVRVDRLTARAGEPVTVATVLRGAGNLN
                     TAGDPDVSASLPLRSFESPGAVSARNAGLEVRGERRHEKAFVPEVPGSFAIEPIRFSW
                     FDPEDGRFHTQTSDSIRIRVVGGNDSTLASAAPAEPPAAPRGRPGPKGSLSLGPPAGA
                     AGLGAGSLAALAGAALAAGVRRRVRLDPKRRRRSELERLARELAALEGPAASPSAAAA
                     RAAAILGESLGVRYGADVEGRSREETLLLTRKAGADEATVEEARRHLESLDRIAFAPA
                     TPGAASEAIAAARAFVERIAREAS"
     gene            2791..3435
                     /locus_tag="VFM00_04270"
     CDS             2791..3435
                     /locus_tag="VFM00_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497493.1"
                     /translation="MTGDAGVLYDAGTAALGRGSIGEATAFLLAAKRIDPRAGDIRRN
                     LAIAERTVAGARGEELPPMPRGPSVALSAAEGWWLAAILVALGAAAGIAAIVRSERRL
                     PAGRFARWLPRGAAAGIAAGLLLAAWLTAGAASERRHPEAVVVAMSVEARSGVDEAPR
                     APILMRAGERARAGRVHGDDVEVIIGGSPVGWVPRAALWNVADAPRYTAGFRYR"
     gene            3451..4161
                     /locus_tag="VFM00_04275"
     CDS             3451..4161
                     /locus_tag="VFM00_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7497494.1"
                     /translation="MARGVLITGGTGSIGSAVTARFLQDGDAVAVTYRAAVEWERLRA
                     AHPAPAESGQLAGFEADVTDHDSMRGAADGAAARLGGLAVLAHIAGGFEGGTPVEQLS
                     EASVRGMIELNLLSAFWAAKHAIPHLKRSGGGRLLFVSSRGAVQAQAGAAPYAASKLG
                     LHALVATLAQELKKDGVTANAVLPSLVDTPQNRAAMPKADVATWVRPEQIAALLSYLG
                     SEASAAVTGALIPIYGKA"
     gene            4165..5292
                     /gene="speY"
                     /locus_tag="VFM00_04280"
     CDS             4165..5292
                     /gene="speY"
                     /locus_tag="VFM00_04280"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008186203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HET7497495.1"
                     /translation="MASSPKSPRYSKGPSIAPDPIRPGITVDRLVDSAFLAYNGARLR
                     EACRLLVEKMLEPDVTVGLTLSGALTPAGMGRSCLIPLLKAGFVDWVISTGANLYHDT
                     HFGIGLTMHQGRANADDVVLREQGIVRIFDIFFDYDVLLRTDAFFREVMRQPEFHRPM
                     GTAEFHYRVGRYVAERERALNQGEVSLVAAAHRLEVPLYTSSPGDSSIGMNAAALSLE
                     GEPIQWDVFRDVNETAAIVYGAKKSGGKSAILILGGGSPKNFALQTEPQIQEVLGLEE
                     AGHDYFLQLTDARADTGGLSGATPAEAVSWGKIDPEKLPDAVVAYVDSTVALPILTAY
                     ALSKHAERPLKRLYGKRDALLNEIKSAHAVRLAAEATHERR"
     gene            5279..6436
                     /locus_tag="VFM00_04285"
     CDS             5279..6436
                     /locus_tag="VFM00_04285"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET7497496.1"
                     /translation="MSAGKLAATAAPLEAARPIPLLDLARAHAPIAAELARDFERVLA
                     SGQFILGAEHDAFERELAAAVGARHAIGVSSGTSAISIGLAALGVGPGDEVIVPAFTY
                     FATASAVVAAGARPVFADVEPRRFGLDPAAMEAAITPRTRAIVPVHLYGLACALAPLL
                     AIAERRGIPILEDAAQAIGATDAGRPVGASSAGATLSFFPTKNLGALGDAGAILTEKD
                     DVAARARLLRVHGDAGNYRHTALGTNARLDALQAALLRTKLRHLEAWTGQRRDTAARY
                     REALAGTPVILPEEPAGARHTYHQFTIRAPERDALQKRLAEQGIGSRVYYPIPLPAQP
                     VFAEEGWRMEAFPVSQTLAREVLSLPIFPGLTPGEVDRVAGAIRAFYGERR"
     gene            6436..>7649
                     /locus_tag="VFM00_04290"
     CDS             6436..>7649
                     /locus_tag="VFM00_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497497.1"
                     /translation="MAVSGGGWPKPLRIALFAAGGFVALCVLVVGLAWALMPRDWIQQ
                     EARRQAAHMSNARVAWARITPEFRDWSLGVTVEKLDLRMPETGPPRLQARLPEAFIRF
                     RLLPLLFRRVEVSGARVKGGGIVFTDQAPSAPSPGESKGGGAHVYVPRVDLDGVSLRT
                     RDPLGSGFDIKRVSGRAEIEGALDHPASLKVEAKADSLYWKPESRSPLLPLPSPAEVK
                     LALDAKDGGQRLVVSDGSATVGPLVSAISGEIRLPPAPAPAQLALRITGQPQTIRSSD
                     PALRAFASQSPASFSATVSWDVRVGGTARDVTNEGRLLLKPFSVSAQSNTFTMDQASV
                     SWSSAADQTFVGRAEGFGGGVTFTAEARGVTRPGGEIRGTFYARAPAERLNGLTANGP
                     MWSSGDLECRGT"
BASE COUNT         1014 a         2761 c         2841 g         1033 t
ORIGIN      
        1 gctggggatg ggcacgccgc aggggacgac gatcccgatc gtcgactcga cgggagtggt
       61 ggtcggcaag cgccgcgatc ccgaggggaa ggtcgtgcag acgaagctgg acgaggcgct
      121 gctccgcgat ctcgccaagc gcggcggcgg cacctacgag cgcggggacg gcaccggccg
      181 cgcggcgctc cacctggccg acgcgatgcg ctccgagggc ggcgtggagg cgcgcgggca
      241 gacggtgcgc gcctacgacg agcgattcgc gtggtttgcg gcgggcgccg ggctcctcct
      301 cgtcctggag cggctcgtgc cgcgccggag gaagcggtga agcgtgccct gctgctcgtc
      361 ctgctcgccg cgagcacgct cggggccttc tacgaatggg gcggccccgc gcggcgcggc
      421 gtccgccagc tgaagaaggg ggaggacgcc gcggcgctcc actccttcgg cgaggcgcgc
      481 cccgagcagc ccacgtcggc ggcggtccgc tacgaccagg ggatcgcgtt ccggaagctg
      541 gggctgaccg actcgtcgag cacggccttc cgcgacgcga tgaagctcca gggaacggcc
      601 gcgcggagcg ccgccggcta caacctgggc aacgaagcgc tccggaagaa ccagctggac
      661 gaggcgatcg agggctacaa ggagtcgctg cgcgccgacc cgcgccgcgc cgacgcaaag
      721 aagaatctcg aggaggcgct ccgccggctg cgcgaggcga agccaccgcc cacgccgccc
      781 agcaagggcg gctcgggctc caagggcccg tccgggcccg gctcgggggg cgggggaggc
      841 ggcggcaatc cgcagccgaa cgctccgccc tcgctgggcc agcagaacac gccgccctcg
      901 gcgtcgggcc cgatcccgag ccgctcggac gcggagctct ggctggacgc gctcgagtcg
      961 gagcggcgct cggagcgcca gcgcgagaag cagcagagcg agccgcgccg ggagaacgtc
     1021 cgtgactggt agcgcgcggg tccggttcgc ggccgtagca ctcctcctcg cggtgtccgc
     1081 gcccgcgcgc gccgcggcgc cacgcctctc catcgaggca cagctcgaca aggaccacgt
     1141 cgcggtgggc gaggaggcgc agatcaccgt ggtggctgag gcgcgcggcg tgaacctgcc
     1201 ggactacgcc ctcccctcgg tgccggggct tcacatcgtg cgcgtcgcca actcccagaa
     1261 cttctcctgg atgaatggga acctctcgcg gtcgaccacg agcatcttcc tcgtgagcgc
     1321 cgccgctcca gggcgctaca cgattccgcc tgtggcgatc gtctcgggga gagagcgcgc
     1381 gcagacgcgg ccgatcgtgc tcgacgttgg cggcgctacg gcgcaggccc cgggtgcgcc
     1441 cggagcgcct ccgggcgaag cgattcctcc acgggtgtgg ggcgacgaga gccttcccga
     1501 gatgttcgtc cggctggtcg tggaccggcg ccgcgcctac tggaaccagc agatcacggc
     1561 ccgctttgtt ctcttcgcgc gcgaccgcct cgaagaagtc ccgatgtggg aggtggccga
     1621 ggcgaacggc ttctggaagg agtcgctcgg cgagatgaag cgcggccgcg tgcgggtcgg
     1681 caaggaggac tacatcgcct acgaacagga cgtggcctac ttcccgacgc gcacggggaa
     1741 gctcaccctg gggcccggcc gcatcgaggc gcgcgtcgcc cgcgcgacga cggcgcccga
     1801 tccctggagc ctcctcggtt tccccgagac gcaggtggag acgatcccgc tccagaccga
     1861 gagcgccacg atcaccgtcc tcccgctgcc cgagggggct ccgccgggct ttcggggcgc
     1921 ggtcgggcgc ctgtcgatgg acgtccgggt ggaccgtctc accgcgcgcg ccggagagcc
     1981 ggtcacggtg gcgacggtcc tccgcggcgc gggaaacctg aacaccgcgg gggatcccga
     2041 cgtcagcgcg tcgctcccgc tccgctcctt cgagtcccct ggggcggtga gcgcgcggaa
     2101 cgcgggactc gaagtccgcg gtgagcgccg tcacgagaag gcgttcgttc ccgaagtccc
     2161 cggctccttc gccatcgagc cgatccgctt ctcctggttc gatcccgagg acgggcgctt
     2221 ccacacccaa acctccgact cgatccggat ccgcgtcgtc ggggggaacg actccacgct
     2281 ggcctcggcc gcgccggcgg agccgcccgc ggccccgcgc ggaaggccgg gtcccaaggg
     2341 ctcgctctcg ctgggccccc cggccggagc ggccgggctg ggcgccggct cgctcgcggc
     2401 gctcgcgggg gcggcgctgg cggccggcgt gcgccggcgc gtccgcctcg acccgaagcg
     2461 ccgccggcgc tcggagctgg aacgcctggc gcgcgagctg gccgcgctcg aggggccggc
     2521 ggcatcgccc tcggccgcgg ccgccagggc ggcggcgatc ctcggcgaat cgctcggcgt
     2581 ccgctacggc gccgacgtgg aggggcgctc gcgcgaggag acgctgcttc tcacgcgcaa
     2641 ggcgggcgcg gacgaggcca cggtcgagga ggcgcggcgc catctggaat cgctggaccg
     2701 gatcgcgttc gcgccggcga cgcctggcgc cgccagcgag gcgatcgccg cggcgcgcgc
     2761 gttcgtcgaa cggatcgcgc gggaggcgtc gtgaccggag acgcgggcgt cctctacgac
     2821 gcgggcaccg ccgccctcgg acgcggctcg atcggcgaag ccaccgcgtt cctgctcgcc
     2881 gcgaaacgga tcgatccgcg cgcgggcgac atccgccgaa acctggccat cgcggaacgg
     2941 accgtggcgg gcgctcgcgg agaggagctt ccgcccatgc cgcgcggtcc ctccgtcgcg
     3001 ctgtcggcgg ccgagggatg gtggctggcc gcgatcctcg tcgcgctggg agcggccgcg
     3061 ggcatcgcgg cgattgtgcg gtcggagcgc cgccttccgg cggggcgctt cgcgcgctgg
     3121 cttccgcgag gggccgccgc gggcatcgcg gcggggctcc tcctggccgc gtggctcacg
     3181 gcgggagcag cctccgaacg gcgtcatccg gaggcggtcg tggtggcgat gagcgtcgag
     3241 gcgcggagcg gcgtggacga agcgccccgc gcgccgatcc tcatgcgggc cggtgagcgc
     3301 gcccgcgccg ggcgcgtcca cggcgacgac gtcgaggtga tcatcggggg aagtcctgtc
     3361 ggatgggtgc cgcgagcggc gctctggaac gtcgccgacg ccccacgcta tactgccggc
     3421 ttccgttacc gttagcggga caggaggctc atggcgcggg gcgtgttgat caccggcggg
     3481 accggctcca tcgggagcgc cgtgacggcg cgctttctcc aggacggcga cgccgtggcg
     3541 gtgacttatc gcgcggccgt cgagtgggag cggctccgcg cggcgcaccc cgccccggcc
     3601 gaatccgggc agctggccgg attcgaagcc gacgtcaccg accacgattc gatgcgcggg
     3661 gcggcggacg gcgcggccgc gcggctgggc ggcctggccg tgctcgcgca catcgccggc
     3721 ggcttcgaag gcggcacccc ggtcgaacag ctctcggagg cttcggtgcg cggcatgatc
     3781 gagctgaatc tcctctccgc gttctgggcc gccaagcacg ccatccctca tctcaagcga
     3841 tcgggcggag gaaggctcct cttcgtctcc tcgcgcggcg cggtgcaggc tcaggcggga
     3901 gcggcgccct acgcggcgtc caagctcggg ctgcatgcgc tggtcgccac gctcgcgcag
     3961 gaattgaaga aggacggcgt caccgccaat gcggtgctcc cgagcctcgt cgacacgccg
     4021 cagaaccggg ccgcgatgcc gaaggccgac gtcgcgacct gggttcgtcc cgaacagatc
     4081 gcggcgctcc tttcgtatct cggctcggaa gcgtcggccg cggtcacggg agcgctcatc
     4141 cccatctacg gaaaggcctg agccatggct tcgtcgccga aatcgccgcg ctactccaag
     4201 ggaccctcga tcgctcccga tccgatccgc cccggcatca cggtggaccg gctggtggac
     4261 agcgcgttcc tcgcctacaa cggcgcgcgc ctccgggagg cgtgccgcct gttggtggag
     4321 aagatgctcg agccggacgt cacggtaggg ctcacgctga gcggcgcgct gacgcccgcg
     4381 ggcatggggc gctcgtgcct gattccgctg ctcaaggccg gattcgtcga ctgggtgatc
     4441 tcgacgggcg cgaatctcta ccacgacacc catttcggca tcgggctcac gatgcaccag
     4501 gggcgcgcca acgccgacga cgtcgtgctc cgcgagcagg ggatcgtccg catcttcgac
     4561 atctttttcg actatgacgt gctgcttcgc accgatgcct tcttccgcga ggtgatgcgc
     4621 cagccggagt tccaccggcc gatggggacg gcggagttcc actaccgcgt ggggcgctac
     4681 gtggccgagc gcgagcgcgc gctgaaccag ggcgaggtct cgctcgtggc cgcggcgcac
     4741 cggctggagg tgccgctcta cacctcgtcg ccgggcgatt cctcgatcgg catgaacgcg
     4801 gcggctctct ccctggaggg ggagccgatc cagtgggacg tcttccgcga cgtgaacgag
     4861 accgcggcga tcgtctacgg cgcgaagaag tcgggcggga agagcgcgat cctgatcctg
     4921 ggcggcgggt cgccgaagaa cttcgcgctc cagaccgagc cgcagatcca ggaggttctc
     4981 ggactggagg aggcggggca cgactacttc ctgcagctca ccgacgcccg cgccgacacc
     5041 ggcggactct cgggtgccac gcccgcggag gcggtgtcgt gggggaagat cgatcccgag
     5101 aagcttcccg acgcggtggt ggcctacgtg gacagcacgg tcgcgctccc gatcctcacc
     5161 gcctacgcgc tctccaagca cgcggagcgc ccgctcaagc ggctctacgg aaagcgcgac
     5221 gcccttctga acgagatcaa gtcggcccat gcggtgcgtc tcgcggcgga ggcgacccat
     5281 gagcgccggt aagctcgcgg ccacggcggc gcccctggaa gccgcgcgcc cgattccgct
     5341 gctcgatctg gcgcgggcgc acgcgccgat cgccgcggag ctggcccgcg acttcgagcg
     5401 ggtgctcgcg tcggggcagt tcatcctggg cgcggagcac gacgccttcg agagggagct
     5461 ggcggccgcc gtgggagccc ggcacgcgat cggcgtctcc tcgggcacct cggcgatctc
     5521 gatcgggctc gcggcgctcg gcgtgggtcc cggagacgaa gtcatcgtgc ccgcgttcac
     5581 ctacttcgcc acggcgagcg cggtggtcgc ggcgggcgcc aggcccgtgt tcgcggacgt
     5641 ggagccgcgc cgtttcggtc tcgatccggc cgccatggag gccgcgatca cgccgcgcac
     5701 gcgcgcgatc gtccccgtgc atctctacgg gctcgcctgc gcgctggcac cgctcctggc
     5761 gatcgcggag cggcggggga tcccgatcct ggaggacgcg gcgcaggcga tcggcgcgac
     5821 cgacgcgggg cgtccggtcg gagcctcgag cgccggtgcc acgctcagct tctttccgac
     5881 caagaacctg ggcgccctgg gtgacgcggg cgccattctc accgagaagg acgacgtggc
     5941 ggcccgcgcg cggctcctgc gcgtgcacgg cgacgcggga aactaccggc acacggcgct
     6001 cggcacgaac gcgcgtctcg acgcgctcca ggccgcgctg ctccgcacca agctgcgtca
     6061 tctggaggcc tggaccgggc agcgccgcga caccgcggcc cgctatcgcg aggcgctcgc
     6121 cggcacgccg gtgatcctcc ccgaggagcc ggcgggcgcg cgccacacct accaccagtt
     6181 caccattcga gcgcccgagc gggacgcgct ccagaagcgg ctggccgagc agggaatcgg
     6241 ctcgcgcgtc tactatccga tcccgctccc ggcccagccg gtcttcgccg aagagggctg
     6301 gcgcatggag gcgttcccgg tctcgcagac gctcgcgcgc gaggtcctgt cgctgccgat
     6361 cttcccgggg ctcacgccgg gcgaggtgga tcgcgtggcg ggcgcgatcc gcgccttcta
     6421 cggggagcgg cgctgatggc cgtttccggc gggggctggc ccaagccgct tcgcatcgcc
     6481 ctgttcgctg cgggcggctt cgtcgcgctc tgcgtgctcg tcgtgggtct cgcctgggcg
     6541 ctcatgccgc gcgactggat ccagcaggaa gcgcggcggc aggccgccca catgagcaac
     6601 gcgcgcgtgg cgtgggcgcg catcacgccg gaattccggg actggtcgct gggcgtcacg
     6661 gtggagaagc tggacctccg gatgcccgag accggcccgc cgcggctcca agcgcgcctg
     6721 cccgaggcgt tcatccgctt ccgcctcctg ccgctcctgt tccggcgcgt ggaagtgagc
     6781 ggcgcgcggg tcaagggcgg cggcatcgtg ttcaccgacc aggcaccctc cgcgccctcg
     6841 ccgggagaga gcaagggcgg cggcgcgcac gtgtacgtgc cgcgcgtgga cctggacggc
     6901 gtctcgctgc gcacccgcga tcccctgggc tcgggattcg acatcaagcg ggtgtccggc
     6961 agggccgaga tcgagggggc gctcgaccac cccgcgtcgc tcaaggtgga ggccaaggcg
     7021 gacagtctct actggaagcc cgaatcgcgc tcgccgctcc tgccgcttcc ctcgccggcc
     7081 gaggtgaagc tggccctgga cgccaaggac ggcggccagc ggctcgtcgt ctccgacggc
     7141 tcggccacgg tcggccccct ggtcagcgcc atctccggcg agatccggtt gcctccagct
     7201 ccggcgcccg cgcagctcgc gctccggatc accgggcagc cgcagaccat acgctcgtcg
     7261 gatcccgcgc tccgcgcgtt cgcttcgcag tcgccggcct cgttcagcgc gacggtctcc
     7321 tgggacgttc gcgtcggcgg caccgcgcgc gacgtgacca acgaggggcg gctgctcctc
     7381 aagcccttct cggtcagcgc ccagtcgaac accttcacca tggaccaggc cagcgtctcc
     7441 tggtcgagcg ccgccgacca gacgttcgtg gggcgggcgg agggtttcgg cggcggcgtg
     7501 acgttcaccg ccgaagcgcg cggcgtcacg cgcccgggcg gcgagattcg cggcaccttc
     7561 tacgcccgcg cgcccgcgga gcgcctgaac ggcctcaccg cgaacggccc gatgtggagt
     7621 tcgggcgacc tggaatgccg cggtacgtt
//
LOCUS       DASSLZ010000116         4051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_39334, whole genome shotgun sequence.
ACCESSION   DASSLZ010000116 DASSLZ010000000
VERSION     DASSLZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39334"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2371)
                     /locus_tag="VFM00_04295"
     CDS             complement(<1..2371)
                     /locus_tag="VFM00_04295"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET7497498.1"
                     /translation="MPDGKQSPNTLSLPFVEEMYAQYLRDPSAVPEEWRSYFQKLAEP
                     NGFAANPRLDPSFPRRALFGRIAERNGMRANAPFISTELEPTFVDRNAPPPAPSRPTA
                     PGERTPASLGATPTRRPAPSGAGPAPFPAQARTGFAPPSAKSAFSLIQAYRSRGHLAA
                     RIDPLGSVRPDPPDLDPAFHGFTEADLDRPVSDTIGGRPAGTLRELVARLREIYCGPV
                     GIQYTHIRDVRGREWLEARQERPEFQTPLQPDEERRVLERLTQAAVFEEFIQRKYIGS
                     KSFSLEGSEALIPLLDVAVESADRHGQNEIVFGMAHRGRLNVLANVLGKEPRQIFYEF
                     EDQDPELYHYRGDVKYHLGYSKDKALPSGRTIHLSLCYNPSHLEFVNPVAIGRLRAKQ
                     DQVGDRARERGMTLLMHGDAAFAGEGIVQETLNLSALPAYETGGTLHVIVNNQIGFTT
                     DPDDARSTPYSTDVARMLQTPIFHVNGEEPDAVIRVTRLAMEFRRAFHRDVVIDLYGY
                     RRHGHNEGDEPAFTQPLLYEAIAKRKPIHRLYAERLVAAGRITAEDAEAMAERMRAFL
                     ESELHETRSVKRTTLPDPPAGPWTRYMGGPESHAPEVATEVEADTLAKLLAAQTVLPE
                     GFTANPKILRGLSQRRQMAEGKRPLDWAAGESLAFATLAVEGYRIRLTGQDTERGTFS
                     HRHAVLHDMKSGAIYTPLHHLSEGQAEVEIRNSPLSEAGCMGFEYGYSLETPDGLVAW
                     EAQFGDFANAGQVIVDQFLTSGEEKWRRLSGLVLLLPHGFEGMGSEHSSA"
     gene            2624..3988
                     /locus_tag="VFM00_04300"
     CDS             2624..3988
                     /locus_tag="VFM00_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET7497499.1"
                     /translation="MLRSIDPATGQVIREYEEAGAGKIASALELADRAGAAWREGSFA
                     DRAAVLGRAAGLLRARAAEYADVMAAEMGKPVTQGKSECEKCAWVCEWFAENGERLLA
                     SDEIPTDASRSYVAYRPLGVVLAVMPWNFPFWQVFRFAAPALMAGNAGVLKHASNVSE
                     CALAIQSVWREAGAPEGVFTTLLVGSAAVGEMIASPHVRAVTLTGSTPAGRSVAAAAG
                     RALKKTVLELGGSDPYVVLEDADLDLAADVCAAARLTNTGQSCIAAKRFIVVDAVREG
                     FTERFVARMRAARFGDPRDTANGLGPLARHDLRDQLHAQVRASIQRGARVLLGGEVPP
                     GPGAYYPPTVLHKVVPGMPAYNEELFGPVAALIAARDEKDAIQIANDSSFGLGSAVFT
                     RDLERGRRIAEHDLEAGSCFVNAQVHSDPRLPFGGIKESGYGRELAAFGIREFTNVKT
                     IYLK"
BASE COUNT          599 a         1330 c         1457 g          665 t
ORIGIN      
        1 gggcgctcga atgctccgag cccatcccct cgaacccgtg cgggagcaag aggacgaggc
       61 cggagagccg gcgccatttc tcctcgccgc tcgtcaggaa ctggtccacg atcacctggc
      121 ccgcgttcgc gaaatcgccg aactgcgcct cccaggccac gagaccatcc ggagtctcga
      181 gcgagtagcc gtactcgaat cccatgcatc cggcttccga gagcgggctg ttccggatct
      241 ccacctcggc ttgcccttcg gacaggtgat ggaggggcgt gtagatggcg ccggacttca
      301 tgtcgtgcag cacggcgtgc cggtggctga acgtgccgcg ctcggtgtcc tggccggtca
      361 gccggattcg atagccctcc acggcgagcg tcgcaaacgc gagcgactcg ccagcggccc
      421 aatccagggg ccgcttcccc tcggccatct ggcggcgctg cgagaggccg cgaagtatct
      481 tcggattggc cgtgaagccc tcggggagca cggtctgcgc ggcgagaagc ttcgcgagcg
      541 tgtccgcctc cacctcggtc gccacctcgg gggcgtggga ctcggggccg cccatgtagc
      601 gggtccaggg tccggcgggc ggatccggga gcgtcgtgcg cttcacgctg cgcgtctcgt
      661 gcagctccga ctccaggaag gcgcgcatgc gctcggccat ggcctcggca tcctcggccg
      721 tgatcctccc cgcggcgacc agccgctcgg cgtagagcct gtggatcggc ttccgcttgg
      781 cgatcgcttc gtacagcaag ggctgggtga aggccggctc gtccccctcg ttgtggccat
      841 ggcgccggta gccgtacagg tcgatcacga cgtcccggtg gaatgcgcgg cgaaactcca
      901 tggccaggcg cgtcacccgg atcaccgcgt ccggctcctc gccgttcacg tggaagatcg
      961 gagtctgcag catccgcgcg acgtcggtgg aatagggcgt cgagcgcgcg tcgtcgggat
     1021 cggtcgtgaa gccgatctga ttgttcacga tcacgtgcag cgtgccgccc gtctcgtagg
     1081 ccggaagcgc gctcaggttc agcgtctcct gcacgatccc ctcgcccgcg aaggccgcgt
     1141 cgccgtgcat caacagcgtc atcccacgct cgcgggcccg gtcgcccacc tggtcctgct
     1201 tcgcgcgcag ccgcccgatc gccaccgggt tcacgaattc caggtggctc gggttgtagc
     1261 agagcgagag gtggatcgtg cgtcccgagg gaagcgcctt gtccttgctg tagcccaggt
     1321 ggtacttcac gtccccgcgg tagtggtaca gctcggggtc ctggtcctcg aactcgtaga
     1381 agatctggcg cggctccttg ccgagcacgt tcgcgagcac gttcaggcgc ccgcggtgcg
     1441 ccatgccgaa gacgatctcg ttctgaccgt gtcggtccgc cgattcgacg gcgacgtcga
     1501 gcagcgggat cagcgcctcc gagccttcca gcgagaacga cttcgacccg atgtacttcc
     1561 gttggatgaa ctcctcgaat acggcggcct gcgtcagccg ctcgagcacg cggcgctctt
     1621 cgtcgggttg gagcggcgtc tggaactcgg gacgctcctg ccgggcctcc agccactcgc
     1681 gccctcgcac gtcgcggatg tgagtgtact gaatgcccac cggcccgcag tagatctcgc
     1741 gaaggcgcgc gaccagctcg cgcaacgtgc cggcggggcg tccgccgatc gtgtcgctga
     1801 ccgggcggtc caggtcggcc tcggtgaagc cgtggaacgc cggatcgaga tcgggcggat
     1861 cggggcgcac cgaacccagg ggatcgatgc gcgcggcgag atgcccgcgc gagcggtagg
     1921 cctggatcag cgagaacgcg gacttggcgc tgggcggggc gaagccggtg cgggcctgtg
     1981 ccggaaaggg cgcgggtccc gcgccgctgg gggccgggcg ccgggtcggc gtcgccccga
     2041 gcgaggccgg ggttcgctcc cccggagccg ttgggcgcga cggtgccggc ggtggggcgt
     2101 tccgatccac gaaggtcggc tccagctcgg tcgagatgaa cggcgcgttc gcgcgcatgc
     2161 cgttccgctc ggcgatgcgg ccgaacaggg cgcgccgcgg gaacgaagga tcgagccggg
     2221 gattggccgc gaagccgttc ggctccgcca gcttctggaa gtagctccgc cactcctcgg
     2281 gcacggccga gggatcgcgg agatactggg cgtacatctc ctccacgaag ggaaggctca
     2341 gggtgttcgg agactgcttg ccgtcgggca tgtcgcgtcg cgttcctggt gttcgggtac
     2401 gaagctatcg ttcaggggga acttggcttg gcactgcgaa agccgtgcca ccactcccct
     2461 ccgcattaga tacgtccgca gagatcgcga tccttgggcg caaggcccaa gccgcttatg
     2521 atgccatgga agcgagcccc gagcatcagg tttagttggc tgcgaacaca cgagttgcgg
     2581 gagccggggt gggcgttccg gccccgtttt ggaggtgagg aaaatgctcc gttccatcga
     2641 cccggctacc ggtcaggtga tccgggaata cgaagaggcg ggcgccggga agatcgcgtc
     2701 cgccctggaa ttggccgacc gggccggggc cgcctggcgg gaggggtcat tcgccgaccg
     2761 cgccgcggtg ctcggccggg ccgcgggtct cctccgcgcg agggcggccg agtacgcgga
     2821 cgtgatggcc gccgagatgg gcaagccggt cacgcagggc aagtccgagt gcgagaagtg
     2881 cgcgtgggtc tgcgaatggt tcgccgaaaa cggcgagcgg ctgctcgcga gcgacgagat
     2941 cccgaccgac gcctcgcgct cgtatgtcgc ctaccggccg ctgggcgtcg tgctcgccgt
     3001 gatgccctgg aatttcccct tctggcaggt ctttcgcttc gccgcccccg cgctgatggc
     3061 gggaaacgcc ggcgtgctga agcacgcctc gaacgtgagc gagtgcgcgc tcgccattca
     3121 gtccgtgtgg cgcgaggccg gcgcgcccga gggcgtgttc acgacgctgc tcgtcggaag
     3181 cgccgccgtc ggcgagatga tcgcctcgcc gcacgtgcgc gcggtgacgc tgaccggcag
     3241 cacgccggcg ggccgatccg tggccgcggc cgcgggccgc gcgttgaaga agacggtgct
     3301 cgaattgggc ggaagtgatc cctacgtcgt gctcgaggat gccgatctgg atctggcggc
     3361 cgatgtgtgc gcggccgcgc ggctcacgaa taccgggcag tcgtgcatcg cggccaagcg
     3421 cttcatcgtg gtggatgcgg tgcgcgaggg gttcacggag cggttcgtgg ccaggatgcg
     3481 cgctgcgcga ttcggcgatc cccgcgatac cgccaacgga ttagggccgc tcgcgcgcca
     3541 cgacttgaga gatcaattgc acgcccaggt gcgcgccagc attcagcgcg gcgcgcgcgt
     3601 gctgctgggc ggcgaggtgc cgccagggcc cggcgcctat tacccgccga ccgtgctcca
     3661 caaggtcgtt ccgggaatgc ccgcctacaa cgaagagctg ttcggacccg tcgccgcgct
     3721 catcgccgcg cgcgacgaga aggacgccat ccagatcgcg aacgactcgt cgttcggttt
     3781 gggttccgcc gtgttcacgc gggacctcga acggggtcgc cggatcgcgg agcacgatct
     3841 cgaagccggc tcctgcttcg tgaacgcgca ggtgcactcg gatccgaggc tgcccttcgg
     3901 cgggatcaaa gagtcaggct atgggaggga gctggccgcg ttcgggattc gagaattcac
     3961 gaacgtgaag acgatctatc tgaagtaaac cgtcgacgcc gtcgggcggg gagtgatcca
     4021 ccggcttgtt tcggtgcttc caggagggat a
//
LOCUS       DASSLZ010000117         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_420057, whole genome shotgun sequence.
ACCESSION   DASSLZ010000117 DASSLZ010000000
VERSION     DASSLZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420057"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..530
                     /locus_tag="VFM00_04305"
     CDS             153..530
                     /locus_tag="VFM00_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497500.1"
                     /translation="MNSIRIAVATLALALGLYAVGCGGNDKNPTGPGPTPADVTINII
                     GNNGSNSYSPSPDTVSVGQTVAWHNSDSMTHTATSTSGTAIGTGGIAPGATSAKVAMN
                     TTGTFNYQCTIHPTMTGILVVKP"
     gene            527..1015
                     /locus_tag="VFM00_04310"
     CDS             527..1015
                     /locus_tag="VFM00_04310"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HET7497501.1"
                     /translation="MRSLLVYLHAASAVALIGELLYAGFWLRSALARGGGSAITRYTL
                     ATMEWTSKSVALPAILVNLITGLGLLHFAHIHFSQSKWVVVSLLLYVILTGVWHGMLI
                     PTRKKMQRLVEAEPAAGYAKEERETSSEFLGMAKRWISVNAVALVLVFGILALMVLKP
                     NF"
     gene            complement(942..2489)
                     /locus_tag="VFM00_04315"
     CDS             complement(942..2489)
                     /locus_tag="VFM00_04315"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HET7497502.1"
                     /translation="MIDGFYLPRALASDVETSVHRFGPAHDAVAIRLPIFLPADVTRW
                     IDRLRRARAEHLEHRPVGEILRPLERVIARHLDPKDRKRRALVQALLESGRYAEPMVQ
                     RALDDAFEPLARGGLRRWIASELGSVSALDRPVPTREGVLRRAVGPEWMFQIYAGNVP
                     AVPVWPMLTALVLKSALLAKTSAQEPVLAPHLARAIADEDEALGRCIAVVWWKGGTAE
                     LERAALAEAPAVLAFGGETAITSLARQTRPDAKAVFHGPKVSLAYVGRGALSRPNLAN
                     AARRLALDVSLYDQQGCLSPHAVYAERGGAVTPLDLAAAIGEALQESAGTIPRREPSA
                     TESAALQLYRAQARFDASASPETNVLASPSGTRWTVVCEGGARFEPGPAHRTVRVHSI
                     TGPEEFERAVGGSTRFIEALALEETNPRRAALVSRFAALGIPRIAGLGSLQRPSPLGT
                     HGGVRRLEPFVTWVTVEEARAPRAAKAPARKTARAANPKKTPRSSASKPSARGSRTPA
                     RARRRSR"
     gene            complement(2486..>2705)
                     /locus_tag="VFM00_04320"
     CDS             complement(2486..>2705)
                     /locus_tag="VFM00_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET7497503.1"
                     /translation="VLDPETMEERHQGEPGILVHYDLANFEVPLAIQTEDVGRRLGEH
                     LILEGRLLDAEPRGCSLAYEEFLKRTGA"
BASE COUNT          417 a          957 c          939 g          392 t
ORIGIN      
        1 tcgagggcat cataccctta cgtatacgtt agattccagg ggaaaagaga ggggtgcagg
       61 cttttgtggt atatagaggt aaggctggca gcacaggctt accggtggtc cgtatctgga
      121 taggccggta accgcaacga ggaggcttct ccgtgaactc gattcggatc gccgtggcaa
      181 ccctggccct cgcactcggc ttgtacgcag tcggttgcgg cggcaacgac aagaatccca
      241 cgggcccggg gcccaccccc gcggacgtga ccatcaacat catcggcaac aacggctcca
      301 actcctattc ccccagcccg gatacggtct cggtcggcca gaccgtggcg tggcacaaca
      361 gcgactccat gacccacacc gccacctcga ccagcggtac ggcgatcggg accgggggga
      421 tcgcgcctgg ggcgaccagc gccaaggtgg cgatgaacac gaccggaacg ttcaactatc
      481 agtgcaccat ccatcccacg atgaccggca tcctcgtcgt caaaccgtga gatcccttct
      541 cgtctacctc cacgccgcga gcgcggtggc gctgatcggc gagctcctgt acgccggatt
      601 ctggctccga tccgcgctcg cgcgcggcgg cggttcggcc atcacccgct acacgctcgc
      661 gacgatggag tggacgagca agtcggtcgc gcttcccgcg atcctcgtca acctgatcac
      721 cgggctggga ctgctccact tcgcgcacat ccatttctcg caatcgaagt gggtcgtggt
      781 ctcgctcctg ctctacgtga tcctgaccgg cgtgtggcac gggatgctga tccccacgcg
      841 gaagaagatg cagcggctgg ttgaggccga gcccgcagcc ggctacgcca aggaagagcg
      901 ggagacctcg agcgagttcc tggggatggc caagcggtgg atcagcgtga acgccgtcgc
      961 gctcgtgctg gtgttcggga tcctcgcgct gatggttttg aagccgaact tctaggcgtc
     1021 ttctttgggt tcgccgcgcg cgcggtcttg cgcgccggcg ccttcgccgc tcgcggcgcc
     1081 cgtgcctcct ccacggtcac ccacgtcacg aacggctcca gccgccgcac gccgccgtgc
     1141 gtgccgagcg gggacgggcg ctgcagcgag cccagccccg cgatgcgcgg gatgccgagc
     1201 gccgcgaacc gggagacgag ggcggcccgg cgcggattcg tctcctcgag cgcgagcgcc
     1261 tcgatgaagc gcgtcgagcc gcccaccgcg cgctcgaact cctcgggccc cgtgatcgaa
     1321 tggacgcgca cggtccggtg cgcgggaccg ggctcgaagc gcgcgccccc ttcgcacacc
     1381 accgtccagc gcgtccccga gggcgacgcg agcacgtttg tctccgggga cgcggaggcg
     1441 tcgaagcgcg cctgcgcgcg atagagctgg agcgcggccg actcggtggc gctgggctcg
     1501 cgccgcggga tcgtacccgc cgactcctgc agcgcctcac cgatcgccgc ggcgagatcc
     1561 agcggcgtga cggcgccgcc ccgctccgcg tagaccgcgt gcggcgaaag gcacccctgc
     1621 tgatcgtaga gcgaaacgtc gagcgcgagc cggcgcgcgg cgttcgcgag gttcgggcgc
     1681 gacagagcgc cgcgccccac gtaggccagg ctcaccttgg ggccgtgaaa gaccgccttc
     1741 gcgtcgggcc gcgtctgccg cgcgaggctc gtgatcgcgg tctcgcctcc gaacgcgagc
     1801 accgccggcg cttcggccag ggccgcgcgc tccagctcgg ccgtcccgcc tttccaccag
     1861 acgacggcga tgcaacgccc gagggcttcg tcctcgtcgg cgatcgcgcg cgcgagatga
     1921 ggcgcgagca ccggctcctg ggctgacgtc ttggccagga gcgccgactt caagacgaga
     1981 gccgtcagca tcggccagac cgggaccgcg ggcacgttcc ccgcgtagat ctggaacatc
     2041 cactccggcc ccaccgcgcg ccgcaggacg ccctcgcgcg tgggcaccgg gcgatccagg
     2101 gccgagaccg agcccagctc cgaggcgatc caccggcgga gcccgccgcg cgcaagcggc
     2161 tcgaacgcgt cgtccagcgc gcgctgcacc atcggctcgg cgtagcgccc ggattccagg
     2221 agcgcctgga ccagggcgcg gcgcttccgg tccttggggt ccaggtggcg ggcgatcacg
     2281 cgctccaggg gccgaaggat ctcgccgacc ggccggtgct ccaggtgctc ggcgcgcgcg
     2341 cggcggagac ggtcgatcca tcgggtgacg tcggcgggaa ggaagatggg gaggcggatc
     2401 gcgacagcgt cgtgcgcggg gccgaagcgg tggaccgagg tctcgacgtc cgacgcgagc
     2461 gcgcgcggca ggtagaaccc gtcgatcacg cgccggtccg cttcaggaac tcttcgtagg
     2521 cgagggagca gccgcgcggc tccgcgtcca ggaggcgccc ctcgaggatc agatgctctc
     2581 ccaggcggcg ccccacgtcc tcggtctgga tcgcgagcgg gacctcgaag ttcgcgaggt
     2641 cgtaatgtac gaggatcccg ggctcgccct ggtgccgctc ttccatcgtc tcgggatcga
     2701 gcacg
//
LOCUS       DASSLZ010000118        15613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1178309, whole genome shotgun sequence.
ACCESSION   DASSLZ010000118 DASSLZ010000000
VERSION     DASSLZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 15613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15613
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178309"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFM00_04325"
     CDS             <1..472
                     /locus_tag="VFM00_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497504.1"
                     /translation="GGLQSPFNPYMSQGYGMQQPYGIGAPGFGLHQGMQQGGFPGITP
                     QVPFGFQHSPFQQQFANPFLSQGLTSPWSQIGSPWSGLGQNNPMGFGGSGGSGGFGGF
                     GGFGGGIGGGLSQLYGGMGGGLYHTPPDLIERQLIEARANDPFRITQTFPFVVS"
     gene            646..1104
                     /locus_tag="VFM00_04330"
     CDS             646..1104
                     /locus_tag="VFM00_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497505.1"
                     /translation="MRRAEHHSRALQTVVVAVVVAALAFAIALATFLPANRDTLRVNL
                     NSIGTSAAEGLTLARGDDESRLTRNFYDVELELLRDDLAHVTTNLTHARPLPELRGHF
                     AEATALAGSASGALRALATPGASAESVIAGQATLGSIKARVDSLKQELER"
     gene            1114..2352
                     /locus_tag="VFM00_04335"
     CDS             1114..2352
                     /locus_tag="VFM00_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET7497506.1"
                     /translation="MSAFLQIFLGILTAMGGFVEVGELVFSVGAGAKFGYSLLWVVAL
                     GTLGIILFGEMAGRVAAVTKQPIFDWVRERAGFRMGLGTLLAANLVSLLTCAAEIGGI
                     AIVLRMMSGWPYRLLAILTFLALLAIVWFFAFKWIERFFGLLGLLMIVFIIAAIRLRP
                     DWHAVAGGFVPNVPAVESSKDYLVYAFFAVSLLSSIMLPYETYFYASGAIEDGWQPHE
                     VPMNRIVVTIGFVLGSVLSVALVLIGAQLLKPLGIDPQWPGAAALGPAFTMGRVGLAV
                     ALLGMLFAFGGAAVENALTGAYNTAQFLGWPWGKWRKPAGAPRFALTWIVMLTLAVLI
                     ILAGVNPIQVVEYSIVFSVLILPLTYLPLLMAAGDKRLMGAYVNNGPTKIAGWFFLGL
                     VTLAGLFALPLLVVTRGGKL"
     gene            2349..2723
                     /locus_tag="VFM00_04340"
     CDS             2349..2723
                     /locus_tag="VFM00_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497507.1"
                     /translation="MKPADLRQRPPFFDVVRMLMDHQLLDADYVECGKVDDVEIEITD
                     DGTLRATAIFTGPGAAFDHLPGWMAAIAKKFFGKRSKRIPWPEIALIESRIKLRSTSE
                     ALGLDPADRSAARWIAKLPGAK"
     gene            2720..2974
                     /locus_tag="VFM00_04345"
     CDS             2720..2974
                     /locus_tag="VFM00_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497508.1"
                     /translation="MKLSEVLGMEVVTASGVHRGHVIDLRSSGEAEHGQPNTARVITE
                     IVIGRVTWLERMGLRSVREEIIPWAEVATVGTRRVTLKNG"
     gene            3081..4475
                     /locus_tag="VFM00_04350"
     CDS             3081..4475
                     /locus_tag="VFM00_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497509.1"
                     /translation="MRQEESSDSGLASSRPGKRPPAIILGDDPVNGLGVARNLGRLGV
                     TVHRVGAAREPLLRSRYLHTQIVVPHLDSMDDDGYARVLERARERIGHDAVLFPLTDL
                     HVLRLCRLEARLAPRFRMTTPPLESAMTLVNKRRFYEAAASFGVDHPATRFPETLEAF
                     DAAAASLGYPVYLKPEISPLYHGKFGRKGFVAADRAELLAGAAQVLEAGLGAIVQEIV
                     PGDAAQMHGCAGYRRGGKSVWVCYRRRREFPEGFGSGTRLTTIPSFAHHTRLIEFLDH
                     VGHQGLFDAEFKLDPRSGKLQLLDVNARAWWQNALPTRCGINMVALAYEDAVKPDSSR
                     SFPAQAYTEGVEWVHGYNDYMAAKDGGMRLGAWLRSVPALGEFAFWAPDDPLPGTLQL
                     SGIAWNMMRGLAGARLPKDTRGYRIAPPPPVAASAAVGPPSTSPVELPTSLSPAPDEP
                     AHAPQEPDPQAALQ"
     gene            complement(4410..5330)
                     /locus_tag="VFM00_04355"
     CDS             complement(4410..5330)
                     /locus_tag="VFM00_04355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7497510.1"
                     /translation="MTPLVSIVIPTYNRSAIVRRAIDSVLAQSFTDLEILVVDDGSTD
                     DTRAALAGYPERVRYVHQENGGPAAARNHGMRLARGEYIGFLDSDDAYLPGNVEAHLR
                     VFERHPEAGLVYCGIEILDRDGKRLKDVLPNPDDRGLVLERLIRYNFITSSTVLMRRA
                     AMEFAGPMSTRLWFAEDWYYWLRVASRFPIEFVNEVLVRYQRSAVSLSHGTAIATIAE
                     RNMEMFALAFDDPDLKPRLKPFKAEAYRRAYAGYASMALEEFQLGLARRFALRAIAAR
                     PTAWSSYPLLLKSSLGVGLLRGMRRLVRGG"
     gene            5427..6704
                     /locus_tag="VFM00_04360"
     CDS             5427..6704
                     /locus_tag="VFM00_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497511.1"
                     /translation="MAAPLSVARADSTWVDRYRAAQAAYRAGDLQGFRAGLMRVAQEI
                     GETPGVNYNLACAEARLGNRSEAIRRLNLYAASGLVSDAAADSDLVSLWADPTFQGIA
                     TRIRQNGDSTGTAREIHRFADSSLLTEDIAYDPTTRSYFATSVHHGRVVEVTASGTER
                     EWTDPDLRPGWGVFAARVDAPRRLLWTSVGAVVTATGYDPADSGRTGLVAWDIRTRRS
                     IQHVEWPRDGKARLLGDITVGPDGTVYACDSPSGAVRILRRGEKELSTLVPDGGFRGP
                     QTPALARDRRRLYVPDYGRGIAIVDIASGTSTWLTFAPGIALQGIDGLYAYGNDLIAV
                     QNGITPRRVTRFRLDGTGTRVVDASVIESGTRRLGEPTHGVIVVKQFVFLGNTGWDRV
                     GRDQQLRPGDAAAPSLLSVELTAAPRLGGKLRR"
     gene            complement(7006..7083)
                     /locus_tag="VFM00_04365"
     tRNA            complement(7006..7083)
                     /locus_tag="VFM00_04365"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7045..7047),aa:Glu,seq:ttc)
     gene            7381..7950
                     /locus_tag="VFM00_04370"
     CDS             7381..7950
                     /locus_tag="VFM00_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497512.1"
                     /translation="MNWLARATAATAAALVLAGCGTTTMGPAAQAPGASAPELVSQSG
                     GTGTFVPAPVLDQEGALASGTLSLAAVTSSATLNGAVGGQLTCGRFAVNVLPGAFVGT
                     GTVTMTMRDPTVAIVDLSIAPSVLNDFKVAVDLSYDPTGLGLTSPVTIYWFDGKKWVD
                     LVAKPDSKTGFPTVHLKHFSPYGAGKAGW"
     gene            7968..8732
                     /locus_tag="VFM00_04375"
     CDS             7968..8732
                     /locus_tag="VFM00_04375"
                     /inference="COORDINATES: protein motif:HMM:NF044948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497513.1"
                     /translation="MLLTVYITLVVGGGGLALWAASAPDPALGLLALLFWLLANLLGE
                     VLWLPDPHGRGYLSMANAANFATLLLLAPRDAVLVTAAAGLLADLFFRKREWYRALFN
                     FGMCAVTVFVASTVFHAAGGRSGPLEALASPLNAAPVLMSAVTYFLVNTVLVAVVIAI
                     SRRQNALRVWRESFAFTYELVGATVLSLLGYLFAILFLIWGYMSAFVAVIATYFIRDA
                     YFRYVADMKAVTAVAPAAGEAKPQPVQGSTPERRAS"
     gene            8729..9400
                     /locus_tag="VFM00_04380"
     CDS             8729..9400
                     /locus_tag="VFM00_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497514.1"
                     /translation="MMRRPNGTYVRHLVALILAASFSVALEGCGEAPTAPVREEVVSG
                     PPLLVVSADGTTNFQRLTATAAADTADTTGSRALKVSKTIDGALGGWVRCGRYFLAFS
                     PGAFDTVGTVTMSMPDSTLMMVDLEIEPARLNGFKAPVYLAANTTDTDVPSDSLAMYW
                     MDPKSGTWTDVTSAKTVTSATDCVTQVDGGLTGDGSPDSNWASLGVTSVLPHFSRYST
                     GKAGW"
     gene            9558..10724
                     /locus_tag="VFM00_04385"
     CDS             9558..10724
                     /locus_tag="VFM00_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7497515.1"
                     /translation="MEKFDGVDFLEIDGLFSEEEIAVRDTIRQFVTDRVMPVIEKHNR
                     EGTFPAHLIPEMAELGVYGANIQGYGCPGLNNVAYGLIMQELERGDSGLRSFVSVQGG
                     LCMYPILAYGSDEQKNKWLPEMAKGKVIGCFGLTEADFGSNPGGMITTAKKDGNGYIL
                     NGSKMWITNGGVAHIAIVWARLDGKIRGFIVENGTPGYSTRDIHGKFSLRASVTSELS
                     FQDCRIPAENILPNVEGLKGPLGCLNQARYGIAWGGLGSAMACYHEALEYSKTRIQFD
                     RPIAGFQMVQEKLAWMIREITKGQLLALQMGRLKDAGTLKPHQVSLGKMNNIEVALKI
                     ARMAREILGASGITDEYQCGRHMCNLESVYTYEGTHDIHTLVIGEAVTGIPAYK"
     gene            complement(10789..11919)
                     /locus_tag="VFM00_04390"
     CDS             complement(10789..11919)
                     /locus_tag="VFM00_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497516.1"
                     /translation="MTRPLARLGAPALCAALLVGFAGPPAAALPLYASREGDVCQTCH
                     VDPNGGGIRNEFGFSYEKNRHSTEPEERWASMTVKPKLNDWITLGVDLRTMYIASHRN
                     GGGRTLETSTFFPMDGQVNIAIMPHEHLTLVASQGLVVDLPGVPTGYVAREIYGMLEG
                     LPGDAYVRVGRFRLPFGLRQDDHTSFVRSLDFLPYDSQKPDAGIEIGHVGAQFFQQIS
                     FTNGTTPFSERAQTVAAKAGIATRTVQAALSGFHRYSDQYLGGRRFERWALYLSATQG
                     RATVLAEAAAGTDDFGDLKRNPGAMFAEMDYRLSRGVDLQGKFDYMDVDRGQSSPWLR
                     RWTGEVLWNPVPFAQASLAYRYNDGAGGGALQEYLAQIYFPF"
     gene            complement(11916..13178)
                     /locus_tag="VFM00_04395"
     CDS             complement(11916..13178)
                     /locus_tag="VFM00_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7497517.1"
                     /translation="MTLPKSAHWKNVGYLLSVECFWGISLALISMVAILPVFLTHLGA
                     SNAALGILPVVWILASSFPGIFASHYTSNQPHRKRAVILLHVVAAVPWLFIAAWFGLA
                     PRPSPLVDITVFIATWGLSWVWMGFTIPVWINFVGKVTRSDMRARSFATIFFFQTLMG
                     AIGGWAASRVLGSGLPFPANYALGFLIAGLCMALGAIFFLPVREEAGAVSEPGQPWPT
                     VVRHAREIWADRAGIRVYLWILFLTTGCYLLITYYPVFAERRFHLTARDSALYTALCM
                     AGQMVGSVLTGLIGDRFGYARVSLIATVALVIGLSLAIWSGHPAVYYVTAFVLGIFLV
                     ADRIALYNLSMAFSPHEDNTAYLGIVPAMVAPLAAIVAGFSGTLIDQFSFVPVAVVGL
                     AGATVALVLALFRLPEPRFSLAGRRNPS"
     gene            13366..14280
                     /locus_tag="VFM00_04400"
     CDS             13366..14280
                     /locus_tag="VFM00_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7497518.1"
                     /translation="MVLAGGISSPAGAVERAAATSSTTTTAAESAASGSGSAALASAA
                     DADSSRTLCVCADPNNLPFSNDREQGFENEIAKLVAKDLGRELRYFWWAQRRGFIRNT
                     LNAHACDVVLGLPAHFDLALTTRPYYRSSYVFVTRRDRNLDLRSLDDARLRSLRIGAH
                     MIGDDYASLPPVQALARRGITGIAGYSIYGDYSKPNPPANLIGAVASGDLDVAVAWGP
                     TAAYFARRSPVRLSVTPIAPDVEEGAPPFSFDIAMGVRKGDVALKAALDGVIARRGKQ
                     IRKILERYDVPLAEKPAEAVTHSELKSP"
     gene            complement(14277..>15613)
                     /locus_tag="VFM00_04405"
     CDS             complement(14277..>15613)
                     /locus_tag="VFM00_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET7497519.1"
                     /translation="RAVRPPLADRMRPRSFDEFEGREALLGPGTLLGTAVQSGNVPSI
                     ILWGPPGSGKTTLARLLAESSRARFEQFSAVTSGVKEVREVIERARAARRANGAPTLL
                     FVDEIHRFNKAQQDAFLPHVEDGTIVLIGATTENPSFEVNNALLSRMKVIVLGMLPPE
                     ALERILRRAMQDSERGLGGSGVSAGDEEIRLVASLSGGDARVALQTLDNAAALASRRK
                     PRVISGEDVREAAQRRTLPYDRVGEEHYNIISALHKALRGSDPDAGLYWLARMLEAGE
                     DPLYVARRLMRFASEDVGLADPEALRLAVAAKDAVHFLGMPEGNTALAQLVVYLALAP
                     KSNSVYLAYGAAAKDALEKPPYPVPLHIRNAPTPLMKGLGYGAGYQYAHQYEGAIDAQ
                     RYFPDEMGAPRYWQGVPRGAEKELAERLERVRKEKERRAGKPPGDEGASGKS"
BASE COUNT         2518 a         5364 c         5230 g         2501 t
ORIGIN      
        1 cggagggttg cagtccccgt tcaatcccta tatgtcgcag ggatacggga tgcagcagcc
       61 ctacggcatc ggtgcgccgg gattcgggct tcaccaaggc atgcagcagg gcggattccc
      121 cggcatcacc cctcaagttc cgtttggctt ccagcacagc cccttccagc agcagttcgc
      181 caaccccttc ctcagccagg gactgacctc gccctggagc cagatcggat cgccctggag
      241 cgggctcggc cagaacaacc ccatgggctt cggcggctcg ggaggctccg gcggatttgg
      301 cggcttcggc ggattcgggg gcgggatcgg gggcgggctg agccagctct acgggggcat
      361 gggcggcggg ctctatcaca ccccgcccga cctgatcgag cggcagctta tcgaggcccg
      421 tgccaacgac ccctttcgga tcacccagac cttccccttc gtcgtctcct gagcggcggc
      481 cctcgccccc cgcaggggtg aagcggcccg ctcctgagac tgcgaagtaa tcggcagatt
      541 tgaagttatc ccgcggggcg ggcgcccggt gcgcctgccc cgctccgttt cgcgtcgaaa
      601 gctacaaagg atgccgggcc ggaagtcctg agctatcatg attcgatgcg tcgagcggag
      661 caccatagcc gcgcacttca gacggtggtg gtcgccgtcg tcgtcgctgc cctcgctttt
      721 gcgatcgccc tggccacgtt cctccccgcc aaccgggaca ccctccgcgt caatttgaac
      781 tcgatcggga cctcggccgc cgaggggctg accctggcgc gcggagacga cgagtcgcgc
      841 ctcacccgca acttctacga cgtcgagctg gagctgcttc gggacgatct ggcccacgtg
      901 accacgaacc tgacccacgc ccgcccgctt cccgagcttc ggggccactt tgcggaagcg
      961 acggccctgg ccgggtccgc ctcgggcgcc cttcgggcgc tggccacgcc gggagcctcc
     1021 gccgagtcgg tgatcgcggg ccaggcgacc ctcggttcga tcaaggcccg ggtcgacagc
     1081 ctgaagcagg agctggagcg ctagctcgtc cccatgagcg ccttcctcca gatcttcctc
     1141 ggcatcctga cggccatggg cgggttcgtg gaggtcggcg agctggtctt ctccgtgggc
     1201 gccggcgcca agttcggcta cagcctgctc tgggtcgtcg ccctggggac gctcggcatc
     1261 atcctcttcg gcgagatggc cgggcgcgtg gccgcggtca ccaagcaacc gatcttcgac
     1321 tgggtgcgcg aacgggccgg gttccgcatg gggctcggca ccctgctggc ggccaacctc
     1381 gtcagcctgc tcacctgcgc cgcggagatc ggcggcatcg cgatcgtgct ccggatgatg
     1441 tccggctggc cctaccggct gctcgcgatc ctcaccttcc tggccctgct cgccatcgtc
     1501 tggtttttcg cgttcaaatg gatcgagcgt ttcttcgggc tcctcggcct cctcatgatc
     1561 gtattcatca tcgccgcgat ccggctgcgt cccgactggc acgcggtggc gggcgggttc
     1621 gtgccgaacg tgccggccgt cgaatcgtcc aaggactatc tggtctacgc cttcttcgcc
     1681 gtgtccctcc tctcctcgat catgctgccc tacgagacct acttctacgc ctcgggcgcc
     1741 atcgaggacg gctggcagcc gcacgaggtg ccgatgaacc gcatcgtggt gacgatcggg
     1801 ttcgtcctgg ggtcggtgct ctccgtggcg ctcgtcctga tcggcgcgca gctcctgaag
     1861 ccgctcggca tcgatcccca atggcccggg gcggccgcgc tcggccccgc cttcaccatg
     1921 gggcgcgtgg ggctcgccgt agcgctcctc ggaatgctct ttgccttcgg cggcgccgcg
     1981 gtcgagaacg cgttgaccgg ggcctataac acggcgcagt tcctgggatg gccctggggc
     2041 aaatggcgga agcccgccgg cgcgccgcgc ttcgcgctga cttggatcgt catgctcacc
     2101 ctggcggtgc tcatcatcct ggccggcgtg aatccgatcc aggtggtcga gtattcgatc
     2161 gtcttctcgg tgctgatcct gccgctcacg tatctcccgc tgctcatggc cgcgggcgac
     2221 aagcggctca tgggcgccta cgtgaataac ggtcccacga agatcgcggg atggttcttc
     2281 ctcggcctgg tcaccctggc cgggctgttc gcgctcccgc tcctggtcgt gacgcgcgga
     2341 gggaagctgt gaagcccgcc gatctccggc agcggccgcc cttcttcgac gtggtgcgga
     2401 tgctgatgga tcaccagctc ctggacgccg actacgtgga gtgcggcaag gtggacgacg
     2461 tggagatcga gatcaccgac gacgggacgc tgcgcgcgac ggccatcttc accggcccgg
     2521 gagccgcgtt cgaccacctg cccggatgga tggcggccat cgcgaagaag ttcttcggca
     2581 agcgttccaa gcggatcccc tggccggaaa tcgcgctgat cgagagccgc atcaagcttc
     2641 ggtccacgtc cgaggcgctg ggcctggacc ccgccgatcg aagcgcggcg cggtggatcg
     2701 cgaagctccc gggggcgaaa tgaagctctc cgaagtgctc ggcatggagg tcgtgaccgc
     2761 ttccggcgtc caccggggcc acgtgatcga cctgcgttcc tcaggcgagg cggagcacgg
     2821 ccagccgaat acggcgcgcg tcatcaccga gatcgtgatc ggccgggtca cctggctgga
     2881 acgcatgggc ctacggtccg tgcgcgagga gatcattccc tgggccgagg tggccaccgt
     2941 cggcacgagg cgcgtcacgt tgaagaacgg ctaagtccct gttctgctat aatcccacct
     3001 gaccccattg tcggcttcgc cggcgtacat atgcccgctg aagggacgcg gcccccacgc
     3061 gcgccaggga gatgcgttcc atgcgtcagg aagaatcctc ggattccggc ctggcctcgt
     3121 ctcgccccgg gaaacgcccc cccgcgatca ttctcggcga cgatcccgtc aacggactcg
     3181 gcgtcgcccg aaacctcggc cgcctcggag tcaccgtcca ccgcgtcggc gccgcgcggg
     3241 agccgctcct ccgctcgcgc tacctccaca cccagatcgt cgtcccccat ctcgattcca
     3301 tggatgacga cggctacgcg cgcgtcctgg agcgggcgcg cgagcggatc ggccacgacg
     3361 cggtcctctt cccgctgacc gatctgcatg tgctgcggct ctgccggctc gaagcccggc
     3421 ttgcgccgcg tttccggatg accacgcctc cgctcgaaag cgcgatgacg ctcgtgaaca
     3481 agcggcgctt ttacgaagcc gcggcatcgt tcggcgtgga tcaccccgcc acgcgctttc
     3541 ccgagacgct cgaagccttc gacgccgcgg ccgcgtcgct gggatacccc gtgtatctga
     3601 agcccgagat ctcgccgctc taccatggga agttcggccg gaagggattc gttgccgccg
     3661 atcgggccga gctgcttgcc ggggccgccc aggtcctcga ggcggggctt ggtgccatcg
     3721 ttcaggagat cgtcccgggc gatgccgcac agatgcatgg ctgcgccggg taccggcgag
     3781 gcggaaagtc ggtgtgggtc tgctaccggc gtcggcgcga gtttcccgag ggcttcggct
     3841 cgggcacgcg cctcacgacc atccccagct tcgcccacca cacgcgtctg atcgagttcc
     3901 tcgaccacgt cggacaccag ggactcttcg acgcggaatt caagctggac ccgaggagcg
     3961 gcaagctcca gctcctggac gtgaacgccc gggcgtggtg gcagaacgcg ctgcccacgc
     4021 gctgcggcat caacatggtc gccctggcct acgaggacgc cgtgaagccg gactcgtccc
     4081 gctccttccc cgcgcaggcg tacaccgagg gcgtggaatg ggtccacggc tacaacgact
     4141 acatggcggc caaggacggc ggcatgcggc tgggcgcatg gctccgttcg gtgcccgcgc
     4201 tcggcgagtt cgcgttctgg gcgccggacg atccgctgcc cggcacgctc cagctctcgg
     4261 gcatcgcgtg gaacatgatg cgcgggttgg cgggcgcgcg gctcccgaag gacacccgtg
     4321 gctaccgcat cgcgccgccg cctccggttg cggcgtcggc tgcggtcggg ccgccctcta
     4381 caagccccgt cgagctgccg acgtcccttt cacccgcccc ggacgagccg gcgcatgccc
     4441 cgcaggagcc cgacccccag gctgctcttc agtagcagcg ggtacgaaga ccacgccgtg
     4501 ggccgcgccg cgatggcgcg gagcgcgaaa cggcgggcca gccccagctg gaactcctcc
     4561 agggccatcg aggcatagcc ggcataggca cggcggtagg cctccgcttt gaacggcttc
     4621 aagcgcggct tcaagtcggg gtcatcgaaa gcgagcgcga acatctccat gttccgctcg
     4681 gcgatcgtcg cgatcgccgt cccatgcgag agcgagaccg cgcttcgttg ataacgcacc
     4741 agcacctcgt tcacgaactc gatcggaaag cgcgacgcga cgcgcagcca gtaataccag
     4801 tcctccgcga accagagccg cgtgctcatc ggccccgcga actccatcgc ggcacggcgc
     4861 atcagcaccg tggacgacgt gatgaagttg tagcggatca gccgctcgag cacgagaccg
     4921 cgatcgtcgg gattcggcag cacgtccttg aggcgcttgc cgtcgcgatc gagaatctcg
     4981 atcccgcagt agaccaggcc cgcctcggga tgccgctcga agacgcggag atgcgcctcg
     5041 acgttccccg gcaggtaggc gtcgtcgctg tccaggaaac cgatatactc tccccgggcg
     5101 aggcgcatgc cgtgattccg cgccgccgcc ggtcccccat tctcctgatg aacgtagcgc
     5161 acccgctccg gatacccggc cagcgcggcg cgcgtgtcgt cggtcgagcc gtcatccacg
     5221 accaggatct cgagatcggt gaagctctgc gcgagcacgc tgtcgatcgc gcggcgcacg
     5281 atggcggatc ggttgtaggt ggggatgacg atgctgacaa ggggggtcac gggcgcatgg
     5341 tagccagttc gaggaagaca gtaaacggag cgttcctgag ggcggcagcg ccggcgctgg
     5401 cggccgcggc actcgcggcg gctgtcttgg ccgcgccgct ctccgtagcc cgcgccgatt
     5461 cgacctgggt cgaccgctac cgcgcggcac aggcggccta ccgggctggc gatctccagg
     5521 gattccgcgc cggcctgatg cgcgtcgccc aagagatcgg cgaaaccccc ggcgtgaact
     5581 acaacctggc ctgcgccgag gcgcggctgg gcaaccgctc ggaggcgatc cgccgtctga
     5641 acttgtacgc cgcctcaggg ctggtgagtg acgcggccgc agactccgat ctcgtctcgc
     5701 tctgggccga cccgacgttc caggggatcg cgacgcggat ccgccagaac ggcgattcga
     5761 ccggcacagc gcgcgagatc caccgcttcg ccgactcctc ccttttgacc gaagacatcg
     5821 cctacgaccc aaccacgcga agctacttcg cgacgagtgt ccatcatggg cgggtcgtcg
     5881 aggtgacggc gtcgggcacg gagcgggagt ggaccgatcc cgatctacgt cccggctggg
     5941 gcgtattcgc cgcgcgcgtc gatgccccac gccgccttct ctggaccagc gtaggcgctg
     6001 tagtgaccgc gacaggatat gaccccgccg actcgggccg cacgggcctc gtcgcctggg
     6061 acatccgcac gcggcggtcc atccagcacg tggagtggcc gcgggacggc aaggcgcgcc
     6121 tgcttggcga catcaccgtc ggccccgacg gcacggtgta cgcctgcgat tcgccatcgg
     6181 gcgcggtgcg catcctgcgc cgtggcgaga aagagctcag caccctcgtc cccgacggag
     6241 gcttccgagg accacagacc cccgccctcg cgcgcgaccg ccgccgtctt tacgtacccg
     6301 actatggccg tggcatcgcc atcgtcgaca tagcctcggg gacgtcgaca tggctcacct
     6361 tcgcgccggg aatcgccctc cagggcatcg acggcctcta cgcctacggc aacgatctga
     6421 tcgccgtgca gaacggcatc acgcctcgac gggtaacgcg cttccggctg gatggcacgg
     6481 gcacgcgagt ggtggatgca tccgtgatcg aaagtggtac gagacgcctg ggcgagccaa
     6541 cgcacggggt gatcgtggtg aagcagttcg tattcctggg caatacgggc tgggatcgcg
     6601 tgggacgcga tcagcagctt cgacctggag acgcggcggc tccgtctctc ctctcagtcg
     6661 aactcacggc ggcgcctcgt ttgggaggaa aactacgccg atagcgaatc gcggatagca
     6721 cacgggcaat cccaacggtc ggccgggggc cgactacatc gtcgcttcct tcaatgagac
     6781 ggcgacgacc gagtcggccc aggcactgat cgattccagg ccaggactcc atgtgagctc
     6841 gatactcgtg ggaggcaaag tcggcattgc gtcggtgaag cctggccagg agcagtactg
     6901 gatcgaggtg ctcaagaagg aatcgatcgt ccggtacgcc acgccgaatt actacgcgta
     6961 cccgtcagcc tcgtagcgcc cctacccatt tcccgtcgtg aaaagtggtg tccccaaggg
     7021 gattcgaacc cctgttgccg ccgtgaaagg gcggtgtcct aggccgggct agacgatggg
     7081 gacaacccga agaagcggtc ttggcgggta gaggcgcacg ccatcggcga ccggcgaata
     7141 taacgtagcg cggccccatc cgcaaacgct cccctctctg gccggatcga gccggccgtc
     7201 gagaaatccc acccagaaat cctgcgtggc tggaatgagc cacggcccga tttgacgatc
     7261 cacgccccac ttacctattt ggacccggcg gcatggccca tgcccttccc tcatggtgac
     7321 ggaagggact gctgtaggca ccgagggcat gggaggcagg gaatgaacgg atcccgaagg
     7381 atgaactggt tggcgagagc gacggccgca accgcggccg ctctcgtcct ggcgggctgc
     7441 ggcacgacga cgatgggccc ggccgcgcaa gcgccgggcg cctccgcgcc cgagctcgtc
     7501 agccagtcgg gcggcaccgg cacgttcgtg ccggctcctg tcctcgacca ggagggcgcg
     7561 ctcgcatcgg gaacgctttc gctggccgcg gtcacctcca gcgccacgct caacggcgcg
     7621 gtgggtgggc agctcacgtg cgggcgattt gccgtgaacg tattgccggg cgcgttcgtc
     7681 ggcacgggca cggtcacgat gacgatgcgc gatccgaccg tcgcgatcgt cgacctgagc
     7741 atcgcgccgt cggttctcaa cgacttcaag gtggctgtgg atctctccta cgacccgaca
     7801 gggctcggcc tgaccagccc ggtcaccatc tactggttcg acggtaagaa gtgggtggat
     7861 ctcgtcgcca agcccgattc gaaaacgggg tttccgaccg ttcacctgaa gcacttctcg
     7921 ccgtacggcg ccggcaaggc cggctggtaa gggagcgagc gaggaccatg ttgctcacgg
     7981 tctacatcac cctggtggtt ggcggcggcg gcctcgccct gtgggcggcg agcgctcccg
     8041 atccggcgct gggacttctc gcgctcctct tctggctgct cgcgaacctc ctgggcgaag
     8101 tgctctggct gcccgacccg cacggccggg gctacttgag catggcgaac gccgcgaact
     8161 tcgcgacgct cctcctgctc gcgccgcggg acgcggtcct cgtgaccgcg gcggcggggc
     8221 ttctcgccga cctgttcttc cgaaagcgcg agtggtaccg ggcgctgttc aacttcggca
     8281 tgtgcgcggt gactgtcttc gtcgcctcga cggtcttcca cgcggccggt gggcgcagcg
     8341 gaccgctcga ggccctggcc tcgcccctga acgcggcgcc cgtcctgatg tcggcggtga
     8401 cctacttcct cgtgaacacg gtgctggtgg ccgtggtgat cgcgatctcc cgccgtcaga
     8461 acgcgctccg ggtctggcgc gagagcttcg ccttcacgta cgagctggtc ggcgcgacgg
     8521 tgctgagcct gctggggtac ctgttcgcga tcctcttcct gatctggggc tacatgagcg
     8581 ccttcgtcgc ggtgatcgcg acctacttca tccgggacgc ctacttccgg tacgtcgcgg
     8641 acatgaaggc ggtcaccgcg gtcgcgccgg cggcagggga agccaagccc cagccggttc
     8701 agggctccac ccccgaacgg agagcctcgt gatgcggcga ccgaacggga cctacgtgcg
     8761 gcacctggtc gcgctgatcc tggccgcctc cttctcggtg gctctggaag ggtgcggcga
     8821 ggcgccgacg gcgcctgtgc gcgaagaggt ggtgtcgggt cccccgctgc tcgtggtgag
     8881 cgcggacggg accaccaact tccagcggct caccgccacg gccgcggccg acaccgcgga
     8941 cacgaccggc agccgcgcgc tcaaggtctc caagacgatc gacggcgcgc tgggcggatg
     9001 ggtccgctgc ggccgctact tcctcgcctt ctcgcccgga gcgttcgata ccgtcggcac
     9061 ggtcacgatg tccatgcccg actcgacgct catgatggtg gatctcgaga tcgagcccgc
     9121 gcggttgaac ggattcaagg cgcccgtcta tctggcggcc aacaccacgg acaccgacgt
     9181 gccgagcgat tcgctcgcga tgtactggat ggacccgaag agcggcacgt ggaccgacgt
     9241 gacctcggcc aagacggtca cctccgccac ggactgcgtg acccaggtgg acggcggact
     9301 cacgggagac gggagcccgg attcgaactg ggccagcctc ggggtcacga gcgtcctccc
     9361 gcacttctcc cgctattcga caggaaaggc gggctggtag cgcgccccct tcgccccccc
     9421 ttccgaggga cggctgcgca cctgcccagg gccgccgcgc aagcggcggc ccgccttgtt
     9481 ttatgtcctc cacccgggta ttctcttccg ccttgcccag cggcgcggtt cgcgcccggc
     9541 tccgtggagg acatccgatg gaaaagttcg acggcgtcga tttcctggag atcgacggcc
     9601 tgttcagcga agaagagatc gcggtgcggg acacgatccg ccagttcgtg accgaccgcg
     9661 tgatgccggt catcgagaag cacaaccgcg agggcacctt ccccgcgcac ctgatccccg
     9721 agatggccga gctgggcgtc tacggcgcga acatccaggg ctacggctgc cccgggctga
     9781 acaacgtcgc ctacgggctg atcatgcagg agctggagcg cggcgacagc gggctgcgct
     9841 cgttcgtctc ggtgcagggc ggcctctgca tgtatccgat cctggcgtac gggtcggacg
     9901 agcagaagaa caagtggctc cccgagatgg ccaagggaaa ggtgatcggc tgcttcgggc
     9961 tcacggaggc cgatttcggc tccaatcccg gcgggatgat cacgacggcg aagaaggacg
    10021 gaaacggcta catcctgaac ggaagcaaga tgtggatcac gaacggcggc gtcgcccaca
    10081 tcgcgatcgt ctgggccagg ctggacggaa agatccgcgg cttcatcgtg gagaatggaa
    10141 cgccgggcta ctccacgcgc gacatccacg gcaagttctc gctgcgcgcc tccgtcacgt
    10201 cagagctctc cttccaggac tgccgcatcc ctgccgagaa catcctgccg aacgtggaag
    10261 ggctgaaggg gccgctcggc tgcctgaacc aggcgcgcta cggcatcgca tggggcgggc
    10321 tgggctccgc catggcctgc taccacgagg cgctggagta ctcgaaaacg cgcatccagt
    10381 tcgaccggcc gatcgccggc ttccagatgg tccaggagaa actggcctgg atgatccgcg
    10441 agatcacgaa ggggcagctc ttggcgctcc agatggggcg gctgaaggac gcaggcaccc
    10501 tgaagcccca ccaggtctcg ctgggcaaga tgaacaacat cgaggtggcg ctcaagatcg
    10561 cgcgcatggc gcgcgagatc ctgggagcca gcgggatcac cgacgagtac cagtgcgggc
    10621 gccacatgtg caatctggag tcggtgtata cctacgaggg aacgcacgac atccacacgc
    10681 tggtgatcgg cgaggccgtg acggggattc cggcgtataa atgattccgc gcggacaggc
    10741 gcgggaccag cggggggcgc cgtgttcgga cggcgcccct tcgcgtttct agaacgggaa
    10801 gtagatctgc gccaggtact cctgaagggc gccgccgccc gcgccgtcgt tgtagcggta
    10861 ggcgagggat gcttgcgcga acggcaccgg attccacagg acttcccccg tccatcgccg
    10921 tagccatggg ctggactgcc ctcggtccac gtccatgtag tcgaacttcc cctgaaggtc
    10981 gacccctcgg ctcagtcggt agtccatctc cgcgaacatc gctccgggat tgcgcttcag
    11041 atctccaaaa tcgtccgtcc ccgcggccgc ctcggcgagg acggtcgcgc gcccctgggt
    11101 cgcggaaaga tacagggccc atcgttcgaa gcgtcgtccc cctaggtact gatccgagta
    11161 gcgatggaac cccgacaacg ccgcttgcac ggtccgtgtc gcaatccccg ctttcgccgc
    11221 aacggtctgg gcccgttccg agaagggcgt ggttccgttc gtgaaggaaa tctgctgaaa
    11281 gaactgtgct cccacatgcc cgatctcgat ccccgcgtcg ggcttctgcg agtcgtacgg
    11341 caggaagtcc aggctccgga cgaacgaagt gtggtcgtcc tgccgcaggc cgaacggcag
    11401 ccggaagcgt cccacccgaa cgtaggcgtc tccgggtagg ccttcgagca tcccgtagat
    11461 ctcccgcgcg acgtatcccg tcgggactcc gggcaggtcc acgaccaacc cctgactcgc
    11521 gacgagggtc aggtgctcat gcggcatgat ggcgatgtta acctgcccgt ccatgggaaa
    11581 gaacgtggac gtctcgagcg tccggcctcc gccgttcctg tgcgaagcga tatacatcgt
    11641 gcgcagatcc actcccagcg tgatccaatc gttcagcttc ggcttcacgg tcatcgacgc
    11701 ccaccgctct tccggctcgg tcgaatgccg gttcttctcg tacgagaatc cgaattcgtt
    11761 ccgaatccct cccccgttcg ggtccacgtg gcaggtctgg cagacatccc cctcgcggga
    11821 ggcgtagagg ggaagcgccg cggcgggagg gcctgcgaat cccacgagaa gagccgcgca
    11881 cagcgcgggc gcgccaaggc gagcgagagg tcgcgtcacg agggattcct ccttccagcc
    11941 agggagaacc gcggttccgg gagccggaac agcgcgagga cgagcgcgac cgtggcgccg
    12001 gccaggccga ccacggccac tggcacgaac gagaactggt cgatcagggt gccggaaaag
    12061 ccggcgacga tcgcggcgag cggcgccacc atcgcgggca cgatgcccag ataggccgtg
    12121 ttgtcctcgt gcgggctgaa cgccatggag aggttgtaga gcgcgatccg gtcggcgacc
    12181 aggaagatgc ccagcacgaa cgccgtaacg taatagacgg cgggatggcc gctccagatg
    12241 gccagcgaca gcccgatcac cagcgcgacg gtcgcgatga gcgagacccg cgcgtagccg
    12301 aaccggtcgc cgatcaagcc cgtcaggacg ctccccacca tctgccccgc catgcagagc
    12361 gcggtgtaga gcgccgagtc gcgcgccgtc aggtggaagc gccgctccgc gaagaccggg
    12421 tagtaggtga tcagcaggta gcagccggtc gtgaggaaca agatccacag atagacgcgg
    12481 attccggcgc gatccgccca gatctcgcgc gcgtgcctca cgaccgtggg ccacggctgc
    12541 cccggctcgc tcaccgcgcc cgcctcctcc cgcaccggga gaaagaagat ggcgcccagg
    12601 gccatgcaga ggcccgcgat gagaaagccg agcgcgtagt tggccggaaa cggcagtccc
    12661 gagccgagca cgcggcttgc ggcccacccg ccgatggcgc ccatcagcgt ctggaagaag
    12721 aagatcgtcg cgaaggagcg ggcgcgcatg tccgagcgcg tcaccttgcc gacgaaattg
    12781 atccagaccg ggatcgtgaa tcccatccac acccaggaaa ggccccaggt cgcgatgaag
    12841 accgtgatgt cgaccagggg cgaggggcgc ggcgccagcc cgaaccaggc cgcgatgaag
    12901 agccacggga ccgcggccac gacgtggagc aggatcacgg cgcgcttccg gtggggttgg
    12961 ttcgacgtgt agtgggaggc gaagatcccc gggaacgacg aggcgaggat ccacaccacg
    13021 gggaggatgc cgagcgcggc gttgctcgct cccaggtgcg tgaggaagac cgggaggatc
    13081 gccaccatcg aaatgagggc gagggagatg ccccagaagc actcgaccga gagaagatag
    13141 ccgacgttct tccagtgggc ggacttggga agcgtcacgc gcgggaagct aacagcaacg
    13201 gccgggagcg gcaatggcgg ctgtattttc ttccatggcg tcgcgcgtgg gacggcatgc
    13261 cctgtcacgc gccaaggagt tgcgcggttc tgttcgaggg cacgcttgat gcaaatggcg
    13321 ccgtccgtga aaccgtttcg ggcactcgcg gcatggcttg gtctgatggt tctggctggc
    13381 ggaatctcca gccccgctgg tgccgtcgaa cgagcggcgg ccacgtcgtc caccaccacc
    13441 accgccgccg agtccgcagc cagcggctcc gggtccgcag ccctcgccag cgcggcggat
    13501 gccgactcca gccgcaccct ctgcgtctgc gccgacccca acaacctccc cttctcgaac
    13561 gatcgcgagc agggcttcga gaacgagatc gcgaagctgg tcgcgaagga cctggggcgc
    13621 gagctccgct acttctggtg ggcgcagcgg cgcggcttca tccggaacac cctgaacgcg
    13681 cacgcctgcg acgtggtcct ggggctgccc gcccacttcg atctggcgct cacgacccgg
    13741 ccctactacc gctcctcgta tgtcttcgtg acgcgacgcg accgaaatct cgatcttcgt
    13801 tcgctggacg acgcgcggct ccgatcgctt cgaatcggcg cccacatgat cggcgacgac
    13861 tacgccagcc ttccgcccgt ccaggcgctg gcgcggcgcg ggatcaccgg gatcgccggc
    13921 tattccatct acggggatta ctcgaagccc aacccgccgg cgaatctcat cggggcggtc
    13981 gcgagcggag atctggacgt cgcggtggcg tggggaccga cggcggccta cttcgcacgg
    14041 cgatcccccg tgaggctctc ggtcacgccc atcgcgccgg acgtcgagga gggcgcgccg
    14101 cccttttcgt tcgacatcgc gatgggtgtt cggaaggggg atgtggcgct gaaagcggct
    14161 ctggacggag tgatcgcgag gcgcgggaag cagattcgaa agatcctgga acgctacgac
    14221 gtgccgcttg ccgagaagcc tgccgaagcg gtcactcact cagaactgaa gtcgccctag
    14281 gacttcccgg aagccccctc gtcgcccgga ggcttccccg cgcggcgctc cttctccttc
    14341 cggacgcgct ccagccgctc cgccagctcc ttctccgccc cccgcgggac gccctgccag
    14401 tagcgcggcg cgcccatctc gtcgggaaag tagcgctgcg cgtcgatcgc cccctcgtac
    14461 tggtgcgcgt actggtagcc cgcgccgtag cccagcccct tcatgagcgg ggtgggagcg
    14521 ttccggatgt ggagcggcac cgggtagggc ggcttctcca gcgcgtcctt ggcggccgcg
    14581 ccgtaggcca ggtagaccga gttgctcttg ggcgcgagcg cgagatagac gacgagctgg
    14641 gcgagcgcgg tgttgccctc cggcattccc aggaagtgca ccgcgtcctt cgcggcgacg
    14701 gcgagccgga gggcctcagg atcggccagc cccacgtcct ccgacgcgaa gcgcatcagc
    14761 cgccgggcca cgtagagcgg atcctccccc gcctccagca tgcgcgcgag ccagtagagc
    14821 cccgcgtcgg ggtcgctccc ccgcagcgct ttgtggagcg ccgagatgat gttgtagtgc
    14881 tcctccccca cccgatcgta gggaagcgtc cgccgctgcg ccgcctcccg cacgtcctcg
    14941 cccgagatca cgcgcggctt ccgccgcgac gcgagcgccg ccgcattatc gagagtctga
    15001 agcgccacgc gggcgtcacc cccggagagt gaggccacga gccggatctc ctcgtcgccg
    15061 gccgagacgc cggatccacc cagcccgcgc tcggaatcct gcatcgcgcg gcgcaggatc
    15121 cgctccagcg cctcgggcgg cagcatgccg agcacgatca ccttcatccg cgagaggagc
    15181 gcgttgttca cctcgaagga agggttctcg gtggtggccc cgatcagcac gatcgtgccg
    15241 tcctccacgt gcggcaggaa ggcgtcctgc tgcgccttgt tgaagcggtg gatctcgtcc
    15301 acgaagagga gggtcggcgc gccgttcgcc cgccgcgcgg cgcgggcgcg ctcgatgact
    15361 tcgcgcacct ccttgacgcc cgaggtcacg gcgctgaact gctcgaagcg ggcgcggctg
    15421 gactcggcga ggagccgcgc cagcgtggtc ttgccgctcc cgggcgggcc ccagaggatg
    15481 atcgagggaa cgttcccgct ctgcacggcg gtgccgagaa gggtgccggg accgaggagc
    15541 gcctcgcgcc cttcgaactc gtcgaaggat cgggggcgca tgcggtcggc gagcgggggg
    15601 cggacggccc ggc
//
LOCUS       DASSLZ010000119        27004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_39795, whole genome shotgun sequence.
ACCESSION   DASSLZ010000119 DASSLZ010000000
VERSION     DASSLZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 27004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27004
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39795"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFM00_04410"
     CDS             complement(<1..724)
                     /locus_tag="VFM00_04410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7497520.1"
                     /translation="MSGARPVWAVVLAFGEYEYTRECLRALLALDPPADRVLLVDNGS
                     PDGTPDRARAEFPEVEVLALGENRWFAGGVNAGLARALQAGAASVLLVNNDLILERGA
                     LGAMVAALEADPRRGAVSPKLFTFEPPDRIWFAGGIVSRGFALIRHRGLGKKDDALIG
                     SGDGPRPVDYVSGAAALLSRSALEKTGLLDESYVIYVEDVDWSARARKAGFVLWYEPA
                     ARGWHHVSVTSGGGLTPLKAYFR"
     gene            complement(721..1860)
                     /locus_tag="VFM00_04415"
     CDS             complement(721..1860)
                     /locus_tag="VFM00_04415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7497521.1"
                     /translation="MKVALLGPTYPYRGGIAHYTTLLARAFAARHDVAMISFTRLYPG
                     FLFPGATQFDASGEAIAPPVAAERVVDSLLPWTWFRAGQRIRAMAADLLIVPWWHPFF
                     GPSLGTAARAARPRGAAGPRRVFLCHNVEPHEAAPGMRALAAYGLGAADGFLVHARSE
                     AKRLERYAGSRPVRVHPHPSYEVFHAAAPDRERARAALEARGRTILFFGYVRPYKGLA
                     DLLAALALARPDAWDLLYVVGEFYEPRERYAEALAHPALRGKVRVVDRYVANEEVAAY
                     VAAADVVALPYREATGSGIAQVAFGGGTPVIATRTGGLEEVVEEGVTGLLVQPGDPPA
                     LARAIERYFDEGLAPRLREGVARARGRFTWEALVDALIELGTECR"
     gene            complement(1897..2901)
                     /locus_tag="VFM00_04420"
     CDS             complement(1897..2901)
                     /locus_tag="VFM00_04420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7497522.1"
                     /translation="MAAEPGGLTGAAPAMTGAMGSDLDRGGAPDLTVLIPVYNEAENM
                     EPLLEKLEADLRPLGLRAEILVVDDGSSDGTAERLRALKPRHRGLRAICFRRNYGKSA
                     ALSVGFAEARGRAIVTMDGDLQDDSAEIGPLLRKLDEGYDLVSGWKQNRQDPVSKTIP
                     SKVFNGVTSAMTHVSLHDMNCGLKIYRAEAAKSVRLRGELHRFIPVLAHWNGYRIGEL
                     PVVHHKRRHGKTKFGAARFVNGFLDLLSVMFLTTSSTRPLHVFGRMGIGVATLGFLFT
                     VWAAWPVVLGEPLRLRPSLVLGLVFIVLGIQFLSLGFLGELIAGTRTTEPEYAIRERI
                     "
     gene            complement(2877..3818)
                     /locus_tag="VFM00_04425"
     CDS             complement(2877..3818)
                     /locus_tag="VFM00_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016953218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7497523.1"
                     /translation="MRALVTGAAGFLGSTLCDALLEEGAEVIGVDAFVPNYPEPVKRR
                     NLARAGAHARFRLVEADLRAADLGALLAGVTHVVHLAALAGVRASWGPAFRDYAEHNV
                     LATQRLLEALRGTRVERVVVASSSSVYGMPERLPTPEDEPRRPLSPYGVTKVATEALL
                     QAYRASCGVPAVGLRYFTVYGPRQRPDMGIHRFFEAARRGDTIAIYGDGEQSRDFTFV
                     DDAIAATRSALVRPAPSLVYNVGGGHRITVNELLGAVERAAGLPVKRRHEPAAAGDPR
                     DTSAETSRALRDLGYRPRTGLDEGLRRQWEWQRNPAD"
     gene            complement(3823..6447)
                     /locus_tag="VFM00_04430"
     CDS             complement(3823..6447)
                     /locus_tag="VFM00_04430"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET7497524.1"
                     /translation="MISWKRAHALVGALAFGVPLISYIRTLTPTVPFWDSGEFIATSY
                     ILGLPHPPGNPVYTMLGRMMTFLPVAGVAWRVNFMSALASALCCLFTFLVTTRALERT
                     FGGRPASAAQWIAARAGGLVATFFIAFASSFWDSAIEAEVYSLSSFMIAFVIWLAFRW
                     WDRIGTARNDFLLVLIAYLLAVSTGIHLGTILVAPGLVLLLAMIRPNAIFNGRFWASA
                     AILGFFLMLVVMNEVEDWGIRNGLFLVMLAAIVAYYGLNRDKMVRNNLATWWVLAIVA
                     GFTVQFFLLVRSQQMPHINEGMPATLETWKDYLLRKQYGPTNPFVRRADLWYQIEHMY
                     LRYVWEQFQLVSRLGSFGETSLPVRLINAIPYFLFFTGGAWNWKRDKKTFWFFAAQHF
                     VMGPMLIFYLNFTDHEVRERDYFFTNSYHLIAIWMGMGATAVLAWVLDAFSSEMSSAR
                     SAEKAAMAGQGGRIPAAGGVPEEAVERPLQPLPPGASTRGAVAVAAFSLIGISLLPAK
                     AGWYTHDRSGFYIAHDYAYNMLVPLQKNAVVFTNGDNDTFPLWYIQEVEKVRPDVRVV
                     NMSLLNTPWYIAQLRDQEPKVPLSYNDTQLAALQPYMDEKTNSVVWVKDQAFADIVKT
                     NAWRQPLYLAVTVPEQEGMDKQLTFEGLVFRIQPHPDPVGIDPKLTYENLYHVYKYRG
                     LLDKNRAYDRSVYKDDNANRLVQNYTAAHVQLATYLIQNKQADQAVRILQDAVKISPD
                     FSGLLEYMGRVYEEAGRPDLARRVYEDGVRRFPNAAEFHYHIGLMLFNEGQVEPGIAE
                     LRKACELSQQFFDWFNALFAALWSTGRREEGVEVLRTWTRAHPDDAQAAGYLQRYEDS
                     LGTGRGGATPLRPPAGAP"
     gene            complement(6444..7511)
                     /locus_tag="VFM00_04435"
     CDS             complement(6444..7511)
                     /locus_tag="VFM00_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET7497525.1"
                     /translation="MSAAAIVKEPGAPGIGGLGPRQKALLAVLKVAISFALFAYVVAK
                     VSPGNVLATMRPADPRLLAAAAGLFLFSSLVGSWLWGRLLRAQGVMIPYPNVAAYYFV
                     GLFFNNFLPSNVGGDIARITDASKYSDRVSPVFSATLMERLIGVVAIALLAVVASFEV
                     LPRLHLTPIYVALVAIFLTSLAAFLSIFSRRVLTVFEWPFRAVGARRVADAMGRLLDD
                     MHGYRSQGGTLLVVFFASSVVQVSRIYVHYLVGLSLGVRIAASYYFVFVPVLAVLVSL
                     PISLNGLGIREGAAVVLFHMVGLTREQSFTIPFLTYVISVVISLLGGLIFLSRSPRRA
                     IAQRLERRRAERAVGAGERRT"
     gene            complement(7508..8620)
                     /locus_tag="VFM00_04440"
     CDS             complement(7508..8620)
                     /locus_tag="VFM00_04440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7497526.1"
                     /translation="MNVLAFNWRDPRHPEAGGAELHLFEILRRCVRDGDRVVWLAERF
                     PGAPAEDRIEGIEIHRAGSWYNAHLALGSLYRRRFRGERFDLVLEDINKVPFFTPLFA
                     GVPVLAVVPHLFGSAVFREAPPAVGLLVWAHESVLPLVYRRTPFLAISESTRDDLARR
                     GIDPRRIRVVRCGLTQNEYGVSTPPADRADPVVVFLGRLRKYKGAQHAIRAMARVRRD
                     VPGARLDVVGDGPYRKDLEALAASLGLRDAVRFLGALPHAEKVRALNEAQVAVCPSPK
                     EGWGLTVIEANACGVPVVASRSPGLVESVREGETGLLVPHGDAEALGLAVVRLLTDRS
                     VRLRMAEAAIRWARTFDWEACYRESRQVMAEAAGAA"
     gene            complement(8617..9324)
                     /locus_tag="VFM00_04445"
     CDS             complement(8617..9324)
                     /locus_tag="VFM00_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7497527.1"
                     /translation="MEQILAQGPVRGRRVLEVGAGSGRDSVALASEGAVSFILDYSMA
                     SLETARKVATRHGAAPILIRADALRLPFREGAFDVIFHQGLLEHFRDPMPLLRENVRA
                     LKGEGLLLVDVPQRWHLYTALKHAMIATGTWFAGWETEFTMGQLERLLRKAGVRVVSR
                     YGAWMVPGLFYRSLRAALLKVKAVRLPLDPPRIPLLSAALQRWRELWDGTPVAFATYF
                     VIGALGRKDSGAGGGAG"
     gene            complement(9417..10142)
                     /locus_tag="VFM00_04450"
     CDS             complement(9417..10142)
                     /locus_tag="VFM00_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7497528.1"
                     /translation="MTTVLLSDIHANLEAFQAVLAAVDARKPDRILCLGDVVGYGASP
                     NECLDLVRSRCEVVLLGNHDAAASGGPEAARFNVYARTAAEWTARTLTRDNREYLQKL
                     PLTAPCASFFCVHASPATPRDWEYLLDRFDAEPQFAYFTEPVCFIGHTHQPAVFMADP
                     DGTRSLPVGTLRIDPARRYIVNVGSVGQPRDRDPRACFVVLREGSGELEYVRVPYDIE
                     GAQAKIRAAHLPEVLAARLSTGE"
     gene            complement(10139..10555)
                     /gene="nusB"
                     /locus_tag="VFM00_04455"
     CDS             complement(10139..10555)
                     /gene="nusB"
                     /locus_tag="VFM00_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET7497529.1"
                     /translation="MNARRLARELAFRACYQSDVTGEPMDRCLGEILDEVGPADETRS
                     FAASLIESLAAHVGEVDAAVSRIARNWPLGRMAATDRSVIRVAAAELLYHGDTPTRVA
                     LDEAIEIAKKYGMDTSGSFVNGILDRIAHEARPSVG"
     gene            complement(10552..11112)
                     /gene="ribH"
                     /locus_tag="VFM00_04460"
     CDS             complement(10552..11112)
                     /gene="ribH"
                     /locus_tag="VFM00_04460"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET7497530.1"
                     /translation="MTQQGRPVPGGSGQDLGPAAGAALRAIRPRFVEGRLDGRGLKVA
                     IACARFNEAVCERLLEGALVELARLGVRTEDIVVARVPGAFELSVAALHLARSGADAV
                     VCLGAVIRGETAHFDFVAGAAAGGIARVAEQTGVPALFGVLTTDTTEQAMERAGGRHG
                     NKGAECAAGAVAMANLVRSIRAARTP"
     gene            complement(11109..12485)
                     /locus_tag="VFM00_04465"
     CDS             complement(11109..12485)
                     /locus_tag="VFM00_04465"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET7497531.1"
                     /translation="MTQAERRALHGADRIHAVETALRAIRAGRMVVVVDDEDRENEGD
                     FVMAAERCTPADVNFMTRDARGLLCVSMPAERLEELGLSLMAPLNTARFATPFTVSVD
                     LLQGTTSGSSAHDRAVTIRALASSRTRPEELARPGHVFPLRAAGGGVLRRPGHTEAAL
                     DLARLAGMKPAGVLCEILSADGRMARGPELARLARRHGMPLITIRDLIRYRYLHETLV
                     ERLAVSKLPTRFGSFRVFVYESRVDGDHHVALVKGTPGKGTPGLIRVHSQCLTGDVFG
                     SMRCDCGEQLAVALDRIDREGCGVFLYMRQEGRGIGLANKLKAYELQDLGLDTVEANL
                     KLGFAADLRDYGIAAQILRDLRFTKVRLLTNNPKKIEALRDYGIDVAAREPIEVRPNA
                     VNERYLLTKRERLGHLLRHPTLTPASNGARRENGVTKAAGARRENGAPKATRARRENG
                     ARRARSAR"
     gene            complement(12508..13113)
                     /locus_tag="VFM00_04470"
     CDS             complement(12508..13113)
                     /locus_tag="VFM00_04470"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET7497532.1"
                     /translation="MFSGIVARVGTVERVWKAAGGTGLAIRSGFDPGSDPEPGASVAV
                     NGVCLTVERVRDGVFEATAVPETVARTTLGGLMAGSRVNLERALRVGDELGGHWVQGH
                     VDAVATVASVEREKDGVRVGIETPEALRHLVAMKGSVAVDGVSLTVAGRTDTRFEVAL
                     IPYTLEHTIAGDYAKGTRVNLEADLIARYLDRLLAAEAPAR"
     gene            complement(13106..14227)
                     /gene="ribD"
                     /locus_tag="VFM00_04475"
     CDS             complement(13106..14227)
                     /gene="ribD"
                     /locus_tag="VFM00_04475"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET7497533.1"
                     /translation="MAEAIRWARRGVGTTHPNPRVGAVVLRGGSVIARGWHERAGEAH
                     AETRALREAGPAAVGATLVVTLEPCAHVGRTPPCVDAIREAKIRRVVVGMLDPNPLVD
                     GKGVEALRRAGIEVALGLREEECRALNPPYLKWRATGLPWVTLKSMVSLDGRVASQNG
                     ESRGLGGPEEQRLVHRLRSESDAVLIGSGTALRDDPLLTARLGRRRKGAKPWRVVLDS
                     ALRTPLDSKLVRTAAAAPLVLATCSPDAWRAREYEARGVVVWRFEPDARGRVPAGEVL
                     RRLAAEGRYAVLVEGGPSVHTTLLREGLADEVAVGIAPVLLGGASAPTWTADLGREGL
                     EEAIPVGPLAVRRLGRDVWLSGPIEPARRTAPREEERHV"
     regulatory      complement(14395..14523)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(14575..17367)
                     /locus_tag="VFM00_04480"
     CDS             complement(14575..17367)
                     /locus_tag="VFM00_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497534.1"
                     /translation="MTRPPAVSILRFRSAILSAAISLVGLVPSARVGAAPAAPSQAVQ
                     APARSLGDVAPVPTIRASLAPALSTADTITISHSDFETFSSPGNEGGWTHVDKSGTPT
                     AWHIAPTVACQGNAFWAGLIDSTWTGDPDRHGYDNNWVQTLSNNVDLTGASSPVKIGF
                     KHRLNLEPGFDFGYLQVNDPVFDEWVGIASFTGKIPNSGVCDTFSVQIPDSIIAKSPV
                     VQFRFVLMTDIQGSSADGLYPDAEGWSVDNVTVRAGAADLRFFDDMESGIGTWSVSTF
                     PAVGDFWRVTNAANTQQLCNSNTSKAWTPTEPLTGNLVPRMDDQLISPRIATNGANQV
                     FLAFDAYRNLPFGGCFYYSVGVRSKQSGLPWSNWSDPTGLLYYGTENEWVRQSVPLAG
                     AAGADSVQVRFEIKDYGAVFCDGVSSAPGTLLYFDNVDVRVLGVAGPSLATSESSLMN
                     DTFKTTTFLGNDNFNTPRGDSTTVRVGASQGLKLAQFIYSLNGAAWTATSLVPVGTAA
                     PDIYYGDVPAGAYPRGTQLRYYFSATDSGNVTATLPVDAVASGHYFTASILPGTYTPT
                     GACPTDSARVLYVNAFAGPDATTGVDQGLAALGVRYDRFDVNSASAGLGNTPGGGDPA
                     SNGVFWPGVSAASLATMYSAIIWDVGERSQLTLSAQDQALLTAWLATSGKNRGLILAG
                     DNLAYDLTANRADTGTFLSCTVGATFVRDIWETSPQDTLLPTLTGASGTRIASEPFAL
                     NGGCPNLNRFDALSVSACAGSGGRAWLSYPNTMMAGTERRAALAGTDSARVVMLGFSL
                     ADIPNAARRNLLLWRTLVEEMETPYCTTPTSVELSEGVPAAPDRIDAPAPNPFNPSTV
                     IRFTVGRPGRVRVTVYNVAGARVRVLVDAPLPSGEHSVRWDGKDDRGRDVGSAAYFIH
                     MDTAEGSRSRKAVLLR"
     gene            complement(17493..18491)
                     /locus_tag="VFM00_04485"
     CDS             complement(17493..18491)
                     /locus_tag="VFM00_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497535.1"
                     /translation="MAERAARWVAVVALLAAGCSGARGPSVHPASGTSSDPAALIAEA
                     DTAIAAADAATARRALDRALALAPESAPVHLARGRFFTAIRRYRDAKSELDRAAALDP
                     RSPEPSYLLGVAYLADGQKEAARSSFARALELDPGHARAREALAGLLEGSYVAAGIPG
                     DYTKLGGHPTVSRGELGVILAVELGADPDRATWRSDEVQRLNWPELDQAWGARWLRAS
                     VVRHWIEAFPDGSLHLDDPLTRGQLALILARLGTESGRWKDSRVGAPDTVFADLGARH
                     YLARAAAEAVRLGLPLREGKFEPLAAATGDEALRSVRGLARLLGAVPVVRTEPGAG"
     gene            complement(18484..19173)
                     /gene="radC"
                     /locus_tag="VFM00_04490"
     CDS             complement(18484..19173)
                     /gene="radC"
                     /locus_tag="VFM00_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET7497536.1"
                     /translation="MEPERRLELHEIDPADRPRERLLELGAGVLTDAELIAVLLGTGS
                     AGTPALAAAARVTGAVNVRRLHQAAPEDLLTIRGVGRARAAQLLAAAELGRRLWPDGD
                     TTPLVRGPESVYELTRDIRAMNREHFVGFYLNSRNQVLRREIISIGSLNASIVHPREV
                     YLPAVAMSAASVVLAHNHPSGDPTPSEEDLAITRRLLEAGRILGIDLLDHVVVARESY
                     ASFKERRLLRG"
     gene            complement(19238..22210)
                     /locus_tag="VFM00_04495"
     CDS             complement(19238..22210)
                     /locus_tag="VFM00_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET7497537.1"
                     /translation="MNNCSIPSGFARLRPTLLAVSVLAVAAAAFSAPAVPPRVPAASP
                     SAKPTPDVIGVVDGVPIMQSEWDRLAAPYFQEVEAKAGRKLSDDEKRLLRKNLLDELI
                     RERLWLADAHRRGMRVNEDVVDARMKENAFFKVKGKVDESKFLAFKRSPTSNYALLRD
                     QVAMGILLEDYARWMERRFGPREPEVRKAYQDLASQASVRYFVLGPEAVSLEPEAEAK
                     QIRAYYDAHQQEFQSPDSAWIQYVRVPIEAASGDSAKDAAVASAQKAASDLVGAMQSG
                     APVETAAKVYGGLHDTGWFRLSDPIRGLGRSEAIQDAIRGAAPGTWVKTPIRAGAHYV
                     VIRLVDRRAARIQSFREVAGQAKRRADAQIREGLADSLGREELRLHPQTYAVPRLAAT
                     VIARSSAEIDTGAAPTAKEIQKLLERRRKEAKIKKSNRAWLDSLAAALPAEVRKQRRD
                     EAIQRAFRETVVRLRGGAPGARVANETGGVAQSVDLYRGQPIEEPILLEGSFLDSLYA
                     MRPGAVVGPRAAHDSLFVVRVDRIDPGFVPPYEAVRQAASSNAQMVKRWALEKDAETY
                     YEAHKEDFKTKPEWIFDYVYFKRAHPDSVPVPDSAIAAYYNEHPLEFTTPATAKPRII
                     LFQYRPSDGPEARDKALQRANAAHDRIAKGEDFAAVAKEVSDDRESGEQGGLIGDVTK
                     SRLMKELADVIFTIPVGQVSDVVEARSAFHLIKVDERVGDTLQPLSQARSEIQTVLGQ
                     PVADSLAFRAASAFIDSLEAGASFDSLAAAHGGAVRSGPTQAGEALPGIGPFETVATA
                     IGDIADGATTPEPVQVGEGYLVARRVREVEPAPAPFSEIKERVIGEYQMSLRRALADS
                     VDQRLREAVAHGENVEALFDGLGGMRMSRSFARSGPIPDFKDRDPRVASDSVLLERIF
                     TSRPGKVLPPVKSSMGTIYMIVESVTVPPAVDFARHRDEVWRDIVDRRIEAWTARLRS
                     RAQVVLYRKDLKSLLAAG"
     gene            complement(22756..24177)
                     /locus_tag="VFM00_04500"
     CDS             complement(22756..24177)
                     /locus_tag="VFM00_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7497538.1"
                     /translation="MARHRPTMLVVDDDPGSSGLLRDIFQQEGYEVTLAARGEEALAL
                     VPARPFDVALCDVQLPDMDGIELLRRIRRAMPGAAVIMVTAFGSYETAITALHEGAFD
                     YVRKPFTLQEVRLTVERAMERRRLQRSAAAAGAASAESTEARPIIGRNPAMVELFKLV
                     SRVAATKSSVLIQGESGTGKELIARTIHQGSPRSSRPFVAVNVTSLSETLLESELFGH
                     VKGAFTGAIERRPGLFVEANHGTIFLDEIGDMSLPMQAKLLRVLQEHELKPVGANETI
                     PVDVRMVTATHQDLDALVRAGRFREDLYYRLNVVTLRVPPLAERPEDIPLLAEHFLRR
                     FGALSNRPLLGFSASAMTALQAYAWPGNVRELENVVDRAVALAPGSIVELTDLPDKLI
                     VHARIGTRESQGGRGEVRRATLDETIQEYVLHVLEEVGGNKTEAARVLGVPRRTLYRM
                     LERYGAVRSRKSKLRRDVPSVSQ"
     gene            24370..25707
                     /gene="mgtE"
                     /locus_tag="VFM00_04505"
     CDS             24370..25707
                     /gene="mgtE"
                     /locus_tag="VFM00_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET7497539.1"
                     /translation="MAPPDSDSEVAERLSVEDLREVWNLLVPEDRIESFRALPRPEAE
                     DLFLELSARDQTELLLALPGPERRSWVRLLPPDDAADLIQEAGAEHRESLLQLLDDPT
                     RKDVHALLAYAEDDAGGLMNPRYARVRPEMSADEALTYLRRQASEQTVSLPYAYVLGP
                     DQKLMGVVSYRELFLARPEQRVQDVMRTDLVTAPESMDQEELSRLFTESRLIAIPVID
                     AEGRMKGIVTLDDIVDVVREEATEDIQKIGGTAAFDEPYLRLSVREMIIKRAGWLSVL
                     FLGESMTAATMGYFEQEIARAVVLALFVPLVISSGGNSGGQATTLVIRAMSLGEVRLR
                     DWWHVMRREILSGLGLGTILAVIGIVRIMVWQAIFHSYGEHHLLIALTVAVSLVGVVM
                     WGTLVGSMLPFILRRLGLDPATASAPFVATLVDVTGLIIYFSAARVFLGGVLL"
     gene            complement(25713..26768)
                     /gene="lipA"
                     /locus_tag="VFM00_04510"
     CDS             complement(25713..26768)
                     /gene="lipA"
                     /locus_tag="VFM00_04510"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET7497540.1"
                     /translation="MSDAERTGTGPDGAARDRAAASDVPRHPISGTAISLTLPSDRRV
                     QAGEKRKPLRSYERQSGDPLPVRRPDWLRARIPTGRNYLETKSILRTLDLHTVCESAN
                     CPNIGDCFSRHTATFLILGNVCTRSCPFCDIRNGKPLPVDPEEPARVADASRRLGLHY
                     IVVTSVNRDELPDGGAMHFAETIRAIHGAIPGARVEVLIPDFLGDEDALRTVLDAKPD
                     VLNHNMETVKRLYKRVRPAGRYERSLQLLKNVATWTPDIPAKSGVMVGVGETQDEVDE
                     LLRDLRDHGVSMVTVGQYLPPSGSHLPLERYVHPDEFRHYREYGLSLGIRQVASGPLV
                     RSSFHAEEQAGETVFVP"
     gene            complement(26761..>27004)
                     /locus_tag="VFM00_04515"
     CDS             complement(26761..>27004)
                     /locus_tag="VFM00_04515"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744151.1"
                     /note="E3 component of alpha keto acid dehydrogenase
                     complexes LpdC; forms a homodimer; binds one molecule of
                     FAD monomer; catalyzes NAD+-dependent oxidation of
                     dihydrolipoyl cofactors that are covalently linked to the
                     E2 component; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7497541.1"
                     /translation="ASILGEGDGFVKAVADAATGEILGVHIIGVQATEQIAESVVARH
                     FEATAEELANVVHAHPTLAEATMEAMFGTEGRAIHV"
BASE COUNT         4137 a         9535 c         9443 g         3889 t
ORIGIN      
        1 gccggaagta ggccttgagc ggcgtcaacc cgcccccgct cgtgaccgac acgtggtgcc
       61 agccgcgcgc ggcgggctcg taccagagaa cgaaccccgc cttccgcgcg cgcgccgacc
      121 agtcgacgtc ctcgacgtag atcacgtacg actcgtccag gagaccggtc ttctcgagcg
      181 cgctccgcga gaggagcgcg gccgcgccgg agacgtagtc cacggggcgc ggtccatccc
      241 ccgacccgat cagggcgtcg tccttcttcc ccagcccgcg atggcggatc agggcgaagc
      301 cgcgcgacac gatcccgccc gcgaaccaga tccggtcggg gggctcgaag gtgaagagct
      361 tgggactcac cgccccgcgg cgcgggtcgg cttcgagcgc cgcgaccatc gcgcccaggg
      421 cgcccctctc taggattagg tcgttgttca cgaggagcac cgatgcggca cccgcctgga
      481 gggcgcgggc gagtcccgcg ttcacgcctc ccgcgaacca gcggttctcc cccaacgcaa
      541 gcacctccac ctcgggaaat tccgcgcgcg cgcgatcggg cgtgccgtcg ggagagccgt
      601 tgtccaccag cagcacgcgg tcggcgggcg ggtccagggc gagcagcgcg cgcaggcatt
      661 cgcgcgtgta ctcatactcg ccgaaggcga gaacgacggc ccagaccggg cgggcgccgc
      721 tcaccggcac tccgttccca gctcgatcag cgcgtccacc agcgcctccc acgtgaaccg
      781 tccccgcgcc cgcgcgacgc cctcgcgcag gcgcggcgcg agtccctcgt cgaagtagcg
      841 ctcgatcgct cgcgccagcg ccggcggatc cccgggctga accagcagtc cggtcacccc
      901 ctcctccacc acttcctcca gcccgcccgt gcgggtcgcg atcaccggag ttccgccgcc
      961 gaaagcgacc tgcgcgatgc ccgaccccgt cgcctcgcgg tacgggagcg ccacgacgtc
     1021 cgcggcggcc acgtacgccg ccacttcctc gttggcgacg tagcgatcca ccacgcgcac
     1081 cttgccgcgg agcgcgggat gggcgagcgc ctcggcgtag cgctcgcgcg gctcgtagaa
     1141 ctcccccacc acgtagagca ggtcccaggc gtcgggtcgc gccagcgcga gcgccgcgag
     1201 gagatcggca agccccttgt acggacgcac gtagccgaag aagaggatcg tccgcccgcg
     1261 cgcctcgagc gcggcgcgcg cccgctcccg gtccggagcg gccgcgtgga acacctcgta
     1321 cgagggatgc gggtgcacgc gcacggggcg gctccccgcg tagcgctcga gccgcttcgc
     1381 ctcggagcgc gcgtgcacca ggaacccgtc cgcggcgccc aggccgtagg cggcgagcgc
     1441 acgcatcccc ggcgccgcct cgtgcggctc cacgttgtgg cacaggaaga cgcgacgggg
     1501 gccggccgcg ccgcggggac gcgcggcgcg ggcggccgtt cccagcgaag ggccgaagaa
     1561 tggatgccac caggggacga tcaggagatc ggccgccatc gcccggatgc gctgccccgc
     1621 ccggaaccag gtccagggaa gcaggctgtc caccacgcgc tcggccgcca ccggcggggc
     1681 gatcgcctcg ccgctcgcgt cgaactgcgt cgcgcccgga aagaggaagc ctgggtacaa
     1741 ccgcgtgaac gaaatcatcg cgacgtcatg gcgcgccgcg aaggcgcggg cgagaagcgt
     1801 cgtgtagtgg gcgatgccgc cgcgataggg gtacgtcggg ccgaggagcg cgaccttcac
     1861 gcggccgccg gcggcgcggg cacggccgcg ccggatctag atccgctccc gaatcgcgta
     1921 ctccggctcc gtcgtgcggg tgcccgcgat cagctcgccc aggaaaccga gcgagaggaa
     1981 ctggatcccg agcacgatga agaccagccc gagcaccagt gagggacgga ggcggagcgg
     2041 ctcgcccagc accaccggcc aggcggccca gacggtaaag aggaaaccga gcgtggccac
     2101 gccgatcccc atccggccga agacgtggag cggacgcgtg gagctggtgg tgaggaacat
     2161 gacggacagg agatcgagga atccgttcac gaagcgcgcc gcgccgaact tggtcttgcc
     2221 gtggcggcgc ttgtggtgga cgacgggcag ctccccgatc ctgtacccgt tccagtgggc
     2281 cagcacgggg atgaagcggt gcagctcgcc gcgcaggcgg accgacttcg cggcctcggc
     2341 gcggtagatc ttgaggccgc agttcatgtc gtggagcgag acgtgcgtca tggccgaggt
     2401 gacgccgttg aataccttcg aagggatcgt cttggagacc gggtcctggc gattctgctt
     2461 ccaccccgag accaggtcgt agccctcgtc cagcttccgc aggagcggcc cgatctcggc
     2521 ggagtcgtcc tggaggtcgc cgtccatggt gacgatggcg cggccgcgcg cttcggcgaa
     2581 gccgaccgac agcgccgcgg acttgccgta gttgcgccgg aagcagatgg cgcgcagtcc
     2641 gcggtggcgc ggcttcaggg cgcgcagccg ctccgccgtg ccgtcgctgg atccgtcgtc
     2701 cacgacgagg atctcggcgc ggaggccgag cgggcggagg tccgcctcca gcttctcgag
     2761 gagcggctcc atgttctcgg cctcgttgta gaccgggatg agcacggtga ggtcgggggc
     2821 gccgcctcga tcgaggtcgg agcccatggc gccggtcatc gccggagccg caccggtcag
     2881 tccgccgggt tccgctgcca ttcccattgc ctccgcaggc cctcgtcgag tccggtgcgc
     2941 gggcggtagc cgagatcccg gagcgcgcgc gacgtctccg cggacgtgtc gcgcgggtcc
     3001 cccgccgcgg ccggctcgtg ccggcgcttc acgggaaggc ccgccgcgcg ctccacggcg
     3061 cccaagagct cgttgacggt gatccggtgg cctccgccga cattgtacac gagtgacggc
     3121 gcgggacgga ccagcgccga gcgcgtggcg gcgatcgcgt cgtccacgaa cgtgaagtcg
     3181 cggctctgct cgccgtcgcc gtagatcgcg atcgtgtccc cgcgccgggc cgcttcgaag
     3241 aagcggtgaa tgcccatgtc gggacgctga cgcggtccgt agaccgtgaa gtagcgcagc
     3301 cccaccgccg ggacgccgca tgaggcgcga taggcctgga gcagcgcctc ggtcgccacc
     3361 ttggtcaccc catagggcga gagggggcgg cgcggctcgt cttcgggcgt cgggagccgc
     3421 tcgggcatgc cgtagaccga ggagctcgac gccacgacca cccgctccac gcgggtgccg
     3481 cgcagggcct cgagcagccg ctgggtcgcc agcacgttgt gctcggcgta gtcgcgaaag
     3541 gccgggcccc aggaagcgcg cacgccggcg agcgcggcca ggtggacgac gtgcgtgaca
     3601 cccgcgagca gcgcgcccag atcggccgcg cggaggtccg cttcgaccag acggaaccgc
     3661 gcgtgcgcgc cggcccgcgc caggttccgc cgcttcaccg gctcgggata attcggaacg
     3721 aacgcgtcca cgccgatcac ctcggcgccc tcctcgagga gggcgtcgca gagggtcgag
     3781 ccgaggaacc ccgccgcgcc ggtgaccagc gcgcgcatgc cgctacggcg ctccggccgg
     3841 cggacggagc ggcgtcgcgc cgccacggcc cgtgccgagc gaatcctcgt agcgctggag
     3901 atagcccgcc gcctgcgcgt cgtcggggtg ggcccgcgtc caggtgcgca gcacctccac
     3961 cccttcctcg cggcgtccgg tgctccagag cgcggcgaag agggcgttga accagtcgaa
     4021 gaactgctgc gagagctcgc aggccttgcg cagctccgcg atgcccggct cgacctgccc
     4081 ctcgttgaag agcatcagtc cgatgtgata gtggaactcg gccgcgttcg ggaaacgccg
     4141 gacgccgtcc tcgtagaccc ggcgcgccag gtccggccgt cccgcctctt cgtagacccg
     4201 ccccatgtac tcgagcaggc ccgagaagtc gggcgagatc ttcaccgcgt cctggaggat
     4261 ccgcaccgcc tgatcggcct gcttgttctg gatcaggtag gtcgcgagct gcacgtgcgc
     4321 cgccgtgtag ttctgcacca gccggttcgc gttgtcgtcc ttgtagaccg aacggtcgta
     4381 ggcgcggttc ttgtccagga gaccgcggta cttgtagacg tggtacaggt tctcgtacgt
     4441 cagcttcggg tcgatcccca cgggatcggg atgcggctgg atgcggaaga ccagcccctc
     4501 gaacgtgagc tgcttgtcca tgccttcctg ctcgggcacg gtcaccgcca ggtagagcgg
     4561 ctgccgccag gcgttggtct tcacgatgtc ggcgaaggcc tggtccttca cccagaccac
     4621 gctgttcgtc ttctcgtcca tgtacggctg cagcgccgcg agctgggtgt cgttgtacga
     4681 gagcggcacc ttgggctcct ggtcgcggag ctgcgcgatg taccacggcg tgttgaggag
     4741 gctcatgttc acgacgcgca cgtcgggacg caccttctcc acctcctgga tgtaccagag
     4801 cgggaaggtg tcgttgtcgc cgttcgtgaa gaccaccgcg ttcttctgga gcgggaccag
     4861 catgttgtag gcgtagtcgt gcgcgatgta gaagcccgag cggtcgtgcg tataccaccc
     4921 ggccttggcc gggaggagcg agatgccgat gaggctgaag gcggcgaccg cgacggcacc
     4981 gcgggtggag gcgccgggcg ggaggggttg aagggggcgt tcgacggctt cttcgggaac
     5041 cccgccggcg gcggggattc gtccgccttg ccctgccatg gccgctttct ccgcgcttcg
     5101 cgcggaggac atctcggagg agaatgcatc cagcacccac gccagcacgg cggtggcgcc
     5161 catccccatc cagatcgcga tcaggtggta ggaattcgtg aagaagtagt cgcgctcgcg
     5221 cacctcgtgg tcggtgaagt tgagatagaa gatgagcatc ggacccatca cgaagtgctg
     5281 ggccgcgaag aaccagaagg tcttcttgtc ccgcttccag ttccaagctc cgccggtgaa
     5341 gaagaggaag taggggatcg cgttgatcaa ccggaccggc agcgacgtct cgccgaagct
     5401 ccccaggcgg ctcaccagct ggaactgctc ccagacgtac cggagataca tgtgctcgat
     5461 ctggtaccag aggtcggccc ggcgcacgaa ggggttcgtc ggcccgtact gcttgcggag
     5521 caggtagtcc ttccaggtct cgagcgtcgc cggcatcccc tcgttgatgt gcggcatctg
     5581 ctggctgcgg acgagcagga agaactggac cgtgaacccg gccacgatcg cgagcaccca
     5641 ccaggtggcc aggttgttgc ggaccatctt gtcgcggttc aagccgtagt aggccacgat
     5701 cgcggcgagc atgaccagga agagcccgtt ccgaatcccc cagtcctcca cctcgttcat
     5761 gacgacgagc atcaggaaga agccgaggat cgcggccgat gcccagaagc ggccgttgaa
     5821 gatggcgttc gggcggatca tggcgagcag gagcaccagg ccgggggcga ccaggatcgt
     5881 gcccaggtgg atgcccgtgg agaccgcgag caggtaggcg atcagcacca ggaggaagtc
     5941 gttccgcgcc gtgccgatcc ggtcccacca ccggaaggcg agccagatca cgaacgcgat
     6001 catgaaggag gagagggagt agacctcggc ctcgatggcg ctgtcccaga agctggaggc
     6061 gaacgcgatg aagaacgtcg ccaccagccc gcccgcgcgc gccgcgatcc attgcgcggc
     6121 cgaagcgggc cgtccgccga acgtccgctc cagggcgcgc gtcgtgacga ggaacgtgaa
     6181 gaggcagcag agcgccgagg ccagcgcgct catgaaattc acccgccacg ccacgcccgc
     6241 caccggcagg aaggtcatca tccgtccgag catggtgtag accgggttcc ccggcggatg
     6301 cgggagcccc aggatgtacg acgtcgcgat gaactccccc gaatcccaga acggcaccgt
     6361 gggggtgagc gtgcggatgt aggagatcag ggggacgccg aacgccagcg cccccaccag
     6421 cgcgtgcgcg cgtttccagg aaatcacgtt ctcctttccc ccgcccccac ggcgcgctcc
     6481 gcacgtcggc gctccaggcg ctgggcgatg gcgcggcgcg gcgaccgcga gaggaagatc
     6541 agccctccga gcaggctgat gacgaccgag atgacgtacg tgagaaacgg gatcgtgaac
     6601 gactgctccc gcgtgagacc gaccatgtga aacagaacca ccgccgcccc ttcccggatc
     6661 ccgagaccgt tcagggaaat cggcagggag acgaggacgg ccagcaccgg cacgaagacg
     6721 aagtagtagc tcgccgcgat ccgcaccccc agggagagcc cgaccaggta gtggacgtag
     6781 atccggctga cctggacgac cgacgaggcg aagaacacga ccagcagcgt gcccccctgg
     6841 gagcggtagc cgtgcatgtc gtcgagcagg cgccccatgg cgtcggcgac ccgacgggct
     6901 ccgaccgcgc ggaacggcca ctcgaatacc gtgagaacgc ggcgcgagaa gatactgagg
     6961 aaggcggcca gggacgtcag gaaaatggcg acgagggcga catagatcgg ggtcaggtgc
     7021 agccgcggca gcacctcgaa cgaggccacg accgcgagga gcgcgatcgc cacgacgccg
     7081 atcaggcgtt ccatcagcgt ggccgagaag accgggctca cccggtcgga gtacttcgag
     7141 gcgtccgtga tccgcgcgat gtccccgccc acgttggacg ggaggaagtt gttgaagaag
     7201 aggccgacga aatagtacgc ggccacgttg ggatacggga tcatcacccc ctgcgcgcgc
     7261 aggagccttc cccagagcca gctccccacc aggctcgaga agaggaagag cccggcggcg
     7321 gcggcgagaa ggcggggatc ggctgggcgc atcgtggcca ggacgttgcc cggcgacacc
     7381 ttggcgacga cgtaggcgaa gagcgcgaag gagatcgcga ccttgagcac cgcgaggagc
     7441 gctttctggc gcggccccag gcccccgatc cccggcgctc ccggctcctt cacgatcgcc
     7501 gcggcgctca cgcggccccc gccgcctccg ccatcacctg gcggctttcg cgatagcagg
     7561 cttcccaatc gaaggtgcgc gcccagcgga tcgcggcctc ggccatccgg agccggaccg
     7621 agcggtcggt gagcagccgg acgacggcca ggcccagcgc ttccgcgtcg ccatgcggca
     7681 cgaggagtcc cgtctcccct tcccgcaccg attccacgag ccccgggctc cggctcgcca
     7741 cgacgggcac gccgcacgcg ttcgcctcga tcacggtgag accccacccc tccttgggcg
     7801 acgggcatac ggcgacctgg gcctcgttca gggcccgcac cttctccgcg tgcggcagcg
     7861 cgccgagaaa gcgcacggcg tcccgaagcc ccagtgatgc cgcgagcgct tcgagatcct
     7921 tccggtaggg accgtcgccc accacgtcca ggcgcgcgcc gggaacgtcg cggcggaccc
     7981 gcgccatcgc gcggatcgcg tgctgagctc ccttgtattt gcggaggcgc cccaggaaca
     8041 ccaccaccgg gtccgcgcgg tcggcgggcg gcgtggacac tccatactcg ttctgcgtca
     8101 agccgcagcg caccacgcgg atccgccggg ggtcgatgcc gcgccgcgcg agatcgtcgc
     8161 gcgtgctctc cgagatggcg aggaagggag tgcggcggta gaccagcggc agcaccgact
     8221 cgtgcgccca gaccaggagt cccaccgcgg gcggcgcctc gcggaagacg gccgagccga
     8281 agagatgcgg cacgacggcg agcacgggaa cgccggcgaa gagcggagtg aagaatggaa
     8341 ccttgttgat gtcctcgagg acgaggtcaa accgctcccc tcggaaccgg cggcgataga
     8401 gtgatcccag ggcaaggtgc gcattgtacc acgagccggc ccggtggatt tcgatcccct
     8461 cgatccggtc ctcggccggc gcgcccggaa agcgctcggc cagccagacc acgcgatcgc
     8521 cgtcgcggac gcagcggcgc aggatctcga agaggtgcag ctccgcgccg cccgcctcgg
     8581 gatgccgcgg atcgcgccag ttgaacgcga ggacgttcac cccgcaccgc cgcccgctcc
     8641 cgaatccttc cgcccgagcg cgccgatcac gaaataggtc gcgaacgcga ccggcgttcc
     8701 gtcccacagc tcccgccagc gctggagcgc ggcggacaga agcgggatgc gcggcggatc
     8761 cagcgggagg cgcaccgcct tgaccttgag gagcgcggcc cggagcgagc gatagaagag
     8821 acccggcacc atccacgcgc cgtagcggct caccacgcgc acgcccgcct tgcggagcag
     8881 ccgttccagc tgtcccatcg tgaactcggt ctcccatccc gcgaaccagg tgccggtggc
     8941 gatcatcgcg tgcttgagcg ccgtgtagag gtgccagcgc tggggcacgt cgacgaggag
     9001 gagtccctcg cccttcagcg cgcgcacgtt ctcgcgcagg agcggcatcg gatcgcggaa
     9061 gtgctcgagc agcccctggt ggaagatcac gtcgaaggcg ccctcccgga acggaaggcg
     9121 cagcgcgtcg gcgcggatca ggatgggtgc ggcgccgtgg cgggtcgcga ccttgcgcgc
     9181 cgtctccagg gaggccatgg aatagtccag gatgaacgag accgcgccct cggaggcgag
     9241 cgccaccgag tcgcggccgg agccggcgcc cacttcgagc acgcggcgcc cccggaccgg
     9301 cccctgcgcc aggatctgct ccacgacgcg gccgtcggtc ggatagaccg cgtcgatgtc
     9361 ctcccgctcc ttccagaagc gggtccagtg ggcttcggtg gacgcgcgca ggagggctat
     9421 tcccccgtgg acaggcgcgc cgcgagcacc tcgggaaggt gcgccgcgcg gatcttggcc
     9481 tgcgctccct cgatgtcgta ggggacgcgc acgtactcca gctcgcccga gccctcgcgg
     9541 agcaccacga agcaggcgcg cgggtcgcgg tcgcgcggct ggccgacgct gcccacgttg
     9601 acgatgtagc ggcgcgccgg atcgatccgg agggtcccga ccgggaggga gcgcgtgccg
     9661 tccgggtcgg ccatgaagac cgcgggctgg tgcgtgtggc cgatgaagca gaccggctcg
     9721 gtgaagtagg cgaattgcgg ctccgcgtcg aagcggtcca gcaggtattc ccagtcgcgc
     9781 ggcgtcgccg gggaggcgtg cacgcagaag aacgacgcgc acggcgcggt gagcgggagc
     9841 ttctggaggt actcgcggtt gtcgcgggtc agcgtgcggg cggtccattc ggccgccgtg
     9901 cgcgcgtaga cgttgaaccg cgcggcctcc gggccgccgc tggcggcggc atcgtggttt
     9961 ccgagcagca cgacttcgca gcgggagcgg accaggtcca ggcactcgtt cgggctcgcg
    10021 ccgtagccga ccacgtcccc caggcagagg atgcggtcgg gcttgcgcgc gtcgacagca
    10081 gccagcacgg cctgaaaggc ttcgagattg gcgtggatgt cggagaggag gacggtggtc
    10141 atccgacgga gggtcgcgcc tcgtgggcga tgcggtcaag gattccgttc acgaacgatc
    10201 ctgacgtatc catgccgtac ttcttggcga tctcgatcgc ctcgtcgagc gcgacgcgcg
    10261 tgggggtgtc cccgtggtag agaagctccg ccgccgcgac gcggatcacc gagcggtcgg
    10321 tggcggccat gcggcccaag ggccagttcc gggcgatgcg ggagacggcg gcgtccactt
    10381 ccccgacgtg cgccgccagg ctctcgatca aggacgcggc gaagctccgc gtctcgtcgg
    10441 cgggccccac ctcgtccagg atctcgccca gacagcggtc catcggctcg ccggtcacgt
    10501 cggactgata gcaggcgcgg aaggccagct cgcgcgcgag gcgccgcgcg ttcatggcgt
    10561 tcgcgccgcc cggatcgagc gcacgaggtt ggccatcgcg accgcgcccg cggcgcactc
    10621 ggcgcccttg ttgccgtggc gcccgcccgc gcgctccatc gcctgctcgg tcgtatcggt
    10681 ggtgagcacg ccgaagagcg cgggcacgcc ggtctgctcg gcgacgcgcg cgatcccgcc
    10741 cgcggccgcg cccgccacga agtcgaaatg cgcggtctcg ccgcggatca ccgcgcccag
    10801 gcagacgacg gcgtccgcgc cgctccgcgc cagatggagc gcggcgacgg acagctcgaa
    10861 agcgcccggc acgcgcgcca ccacgatgtc ctcggtccgc acgcccaggc gggccagctc
    10921 gaccagcgcg ccctcgagaa ggcgctcgca gaccgcttcg ttgaagcgcg cgcacgcgat
    10981 cgcgaccttg aggccgcggc cgtccaggcg cccttccacg aaacggggac ggatcgcccg
    11041 gagcgccgcg ccggccgcgg ggcccagatc ctgtccggag ccgccgggga cgggccgtcc
    11101 ctgctgggtc atcgcgcgct cctcgcgcgg cgggcgccgt tctcgcggcg ggcgcgggtc
    11161 gccttgggcg ctccgttctc gcggcgggcg ccggccgcct tcgtcacacc gttctcccgc
    11221 cgcgcgccgt tggacgcggg cgtgagcgtg ggatggcgca gcaggtggcc cagccgttcg
    11281 cgcttggtca ggaggtagcg ctcgttcacg gcgttgggac gcacctcgat cggctcgcgc
    11341 gcggccacgt cgatgccgta gtcccgcagc gcctcgatct tcttcggatt gttcgtgagc
    11401 agacggacct tggtgaagcg gaggtcgcgc aggatctgcg cggcgatccc gtagtcgcgg
    11461 agatccgccg cgaagcccag cttgaggttc gcctccacgg tgtcgagccc caagtcttga
    11521 agttcgtacg ccttgagctt gttcgcgagc ccgatccccc gcccctcctg gcgcatgtac
    11581 aggaatacgc cgcacccctc gcggtcgatc cggtcgagcg ccacggcgag ctgctcgccg
    11641 cagtcgcacc gcatcgagcc gaacacgtcc ccggtcaggc actgggagtg cacgcggatc
    11701 aggccgggcg tccccttgcc gggcgttccc ttgaccagcg ccacgtggtg atcgccgtcc
    11761 acgcggctct cgtagacgaa cacgcggaac gagccgaatc gggtcggaag cttggagacg
    11821 gcgagccgct ccaccagggt ctcgtgaaga tagcggtagc ggatcaggtc gcggatcgtg
    11881 atgagcggca ttccatggcg ccgggccagc cgcgcgagct cgggtccgcg cgccatgcgg
    11941 ccgtcggccg agaggatctc gcagagcacg cccgcgggtt tcatcccggc gagccgcgcc
    12001 aggtcgagcg cggcctcggt gtgccccggc cggcgcagga cgccgccccc cgccgcgcgg
    12061 agcgggaaga cgtgcccggg ccgcgccagc tcctcgggac gcgtgcgcga cgacgccagg
    12121 gcgcggatcg tcacggcccg gtcgtgcgcg gaggagccgg acgtcgtgcc ctggaggagg
    12181 tcgaccgaca ccgtgaaggg ggtggcgaag cgcgccgtgt tcagcggcgc catgagcgag
    12241 aggcccagct cttcgagccg ttccgccggc atcgagacgc agaggaggcc gcgcgcgtcg
    12301 cgcgtcatga agttcacgtc ggcgggcgtg cagcgctccg ccgccatgac gaagtccccc
    12361 tcgttctcgc ggtcctcgtc gtccacgacg accaccatcc gcccggcccg gatcgcgcgc
    12421 agcgcggtct cgaccgcatg gatgcggtcc gcgccgtgca gggccctccg ctcggcctgg
    12481 gtcatcgtgc tctcctaggg ttccggctca tcgcgccggc gcctcggccg cgagcagacg
    12541 gtccaggtag cgggcgatca ggtcggcttc caggttcact cgcgtgcctt tcgcgtagtc
    12601 gccggcgatc gtgtgctcca gcgtgtacgg gatgagcgcc acctcgaacc gggtgtcggt
    12661 gcgtcccgcc acggtgaggc tcacgccgtc caccgcgacc gagcccttca tcgcgacgag
    12721 gtggcggagc gcctcgggcg tctcgatccc gacgcgcacg ccgtccttct cacgctccac
    12781 cgacgccacg gtcgcgacgg cgtccacgtg cccctgcacc cagtggccgc ccagctcgtc
    12841 gcccacccgg agcgcgcgct cgagattgac gcgggagccg gccatcagcc ctcccagcgt
    12901 ggtgcgggcg acggtctccg gcacggcggt cgcctcgaag acgccgtccc gcacgcgctc
    12961 caccgtgagg cagacgccgt tcacggcgac actcgccccg ggctcggggt ccgaacctgg
    13021 gtcgaatccc gaccggatcg cgaggcccgt tcctccggcc gccttccaga cccgctcgac
    13081 cgttccgacg cgcgccacga tgccgctaaa catgacgctc ctcctcgcgc ggcgcggtcc
    13141 gccgcgccgg ctcgatcggc cccgacagcc agacgtcccg tcccaggcgc cgcaccgcga
    13201 gcggacccac cgggatcgcc tcctcgaggc cctccctgcc gaggtcggcg gtccaggtcg
    13261 gcgccgaggc accccccagg agcaccggcg cgatccccac cgccacctca tcggcgagac
    13321 cctcgcgcag aagcgtggta tgcaccgagg ggcccccctc gacgagcacg gcgtagcggc
    13381 cttcggccgc caggcggcgc agcacctcgc cggccgggac gcgtcctcgc gcgtcgggct
    13441 cgaagcgcca caccacgact ccgcgcgcct cgtactcccg cgcgcgccac gcgtcgggcg
    13501 agcaggtggc gagcacgagc ggtgccgccg ccgcggtccg caccagcttc gaatcgagcg
    13561 gcgtgcggag cgccgagtcg agcaccacgc gccagggctt ggcccccttg cgccggcggc
    13621 cgagccgcgc ggtgagcagc ggatcgtccc gcagcgccgt gcccgagccg atcagcaccg
    13681 catcgctctc ggagcgcagc cggtgcacca gccgctgctc ctcgggaccg cccaatccgc
    13741 gggactcgcc attttgggag gcgacccttc cgtccaggga aaccatcgac ttgagcgtga
    13801 cccaggggag tccggtggcg cgccacttga gatacggcgg attgagtgcc cggcattcct
    13861 cctcgcggag gccgagcgcc acctcgatgc ccgcgcgacg aagcgcctcg acgccctttc
    13921 catccacgag cgggttcgga tcgagcatgc cgaccaccac gcgccggatc ttcgcctcgc
    13981 ggatcgcgtc gacacagggc ggcgtgcggc ccacgtgcgc gcagggctcc agggtcacga
    14041 ccagggtcgc gccgaccgcg gccggacccg cttcgcggag cgcccgcgtc tcggcgtgcg
    14101 cctcaccggc gcgctcgtgc cagccgcgcg cgatgaccga ccccccgcgg agcacgaccg
    14161 cgcccacgcg gggattgggg tgcgtggtgc ccacaccgcg tcgcgcccag cggatcgctt
    14221 cggccatggc gcggtcctcc gcggcgctcg ggcgcgcggc atccgccccc gttcccgtcc
    14281 gggcctcgtt cgttccgatc ggcgatgaaa ggctcaatga aaaaccccgc aggcagacac
    14341 ccgcggggcg gccggaaggc ccatagcgcc aggaagcggc ggacccatcc tacgatcatc
    14401 cttctcccat ccagactgtg actgtcggct ccggaatcgc accggatcct gcccaggacc
    14461 gtggccccgg gctcgcgggc tatgaccgcc ggtggggact ttcaccccgc cccgaaggat
    14521 gtctgctgga acgagaatag ggcctggagg ggccgggcgc aacccccgcc ggatctagcg
    14581 gaggagaacg gctttgcgcg accgggagcc ctctgcggtg tccatgtgga tgaagtaagc
    14641 ggcgctcccg acgtcgcggc cgcgatcatc cttgccgtcc cagcgcaccg agtgctcccc
    14701 ggacggcagc ggcgcgtcca cgaggacgcg gaccctcgcc cccgccacgt tgtagacggt
    14761 cacgcggacc cggccggggc ggcccaccgt gaagcggatc acggtggagg gattgaaggg
    14821 attcggcgcc ggcgcatcga tccgatcggg agcggccggc acgccttcgc tcagctccac
    14881 cgaggtcggc gtggtgcagt agggagtctc catctcctcg accagagtgc gccagagcag
    14941 gagattccgg cgcgccgcgt tcggaatgtc cgccagggag aatccgagca tcacgacgcg
    15001 cgcgctgtcg gtgccggcca gggccgcgcg ccgctccgtc cccgccatca tcgtgttcgg
    15061 atagctcagc cacgcccggc cgccggaccc ggcgcaggcc gacacggaga gcgcgtcgaa
    15121 gcgattcagg ttgggacagc cgccgttcag cgcgaagggc tccgaggcga tccgcgtccc
    15181 ggaggcgccc gtcagcgtgg gaagcagcgt atcctggggc gacgtctccc agatgtcgcg
    15241 cacgaacgtc gcgcccacgg tgcacgagag gaaggtcccc gtgtcggcgc gattcgccgt
    15301 cagatcgtaa gcgagattgt ccccggccag gatcagcccg cggttcttcc cgctggtcgc
    15361 gagccacgcg gtgagcagcg cctggtcctg cgcgctcagc gtcagctgcg agcgctcccc
    15421 cacgtcccag atgatcgccg agtacatcgt ggcgagcgac gccgcgctca cccccggcca
    15481 gaacacgccg ttgctggcgg gatcgccgcc gcccggcgtg ttgccgagac ccgccgaggc
    15541 cgaattcacg tcgaagcggt catagcgcac gcccagggcg gccagcccct gatccacgcc
    15601 ggtcgtcgcg tcgggcccgg cgaacgcgtt cacgtagagc acgcgcgcgc tgtcggtcgg
    15661 gcacgccccc gtgggcgtgt aggtgccggg aaggatgctg gccgtgaagt aatggccgga
    15721 cgccacggcg tcgacgggaa gcgtagccgt gacattaccc gagtcggtgg cgctgaagta
    15781 gtagcggagc tgtgtccccc gcgggtaagc ccctgcgggc acgtcgccgt aatagatgtc
    15841 gggagccgcc gtccccaccg gcacgagcga cgtcgccgtc cacgcggcgc cgttcaagct
    15901 gtagatgaac tgcgcgagct tgagcccctg ggaagccccg acgcgaacgg tggtgctgtc
    15961 gccgcggggc gtgttgaagt tgtcgttgcc gaggaaggtg gtggtcttga acgtgtcgtt
    16021 catcaggctc gactccgacg tcgccagcga cgggcccgcc accccgagca cccggacgtc
    16081 cacgttgtcg aagtagagca gggtcccggg cgccgaggat acgccgtcgc agaagacggc
    16141 cccgtagtcc ttgatctcga agcgcacctg caccgaatcg gcgccggccg cgcccgcgag
    16201 gggcacgctc tgccggaccc attcgttctc ggtgccgtaa tagagcagcc cggtcggatc
    16261 gctccagttg gaccagggca ggcccgactg cttggaacgg actccgacgg agtagtagaa
    16321 gcagccgccg aacggcaggt tccggtaggc gtcgaaagcg aggaatacct ggttggcgcc
    16381 gttggtggcg atgcgcgggg agatcagctg gtcgtccatg cgcgggacca ggttgccggt
    16441 caacggctcg gtgggggtcc aggccttcga cgtgttggag ttgcagagtt gctgcgtgtt
    16501 cgccgcattg gtcacgcgcc agaagtcgcc cacggctgga aaggtcgaca cgctccaggt
    16561 gccgatcccc gactccatgt cgtcgaagaa gcgcaggtcg gcagcgcccg ccctcacggt
    16621 cacgttgtcg accgaccatc cctcggcgtc cgggtagagc ccgtcggccg agctaccctg
    16681 gatgtcggtc atcaggacaa atcggaactg gacgactggg ctcttcgcga tgatcgagtc
    16741 ggggatctgg acgctgaacg tgtcgcagac tccggagttg ggaatcttcc ccgtgaacga
    16801 cgcgatgccg acccattcgt cgaaaaccgg atcgttcacc tgcaggtatc cgaaatcgaa
    16861 gccgggttcc agattcaagc ggtgcttgaa gccgatcttg accggcgagg aggctcccgt
    16921 cagatcgacg ttgttcgaga gcgtctggac ccagttgttg tcatagccgt gccggtcggg
    16981 atcgccggtc catgtcgaat cgatgagccc cgcccagaac gcgttcccct ggcaggccac
    17041 cgtgggagcg atgtgccacg ccgtcggagt gcccgacttg tccacgtggg tccatccccc
    17101 ttcgttgccg ggactggaga aggtctcgaa atcggagtgg gagatcgtga tcgtgtcggc
    17161 cgtgctgagg gcgggagcga gcgaggcgcg gatggtaggg accggcgcga catcgccaag
    17221 gctgcgcgcc ggggcttgaa cggcctggga tggggcggcc ggcgccgccc ccacgcgtgc
    17281 tgacggaacg agcccaacga ggctgatcgc ggccgagagg atcgcggagc ggaagcgcag
    17341 gatgctgacg gccggaggac gggtcacccc gtaattttac cattatccgc gcggccggtt
    17401 ccgagggatc ggcgggcagc gggggcgcgc ctcccaagtc ttgcgcccgc ctcgatctct
    17461 agcgcctcga ccgcggcggc gccacctcga tctcagccgg cgcccggctc cgtccgcacg
    17521 acgggaacgg ctcccaggag gcgcgcgagc ccgcgaaccg atcgaagcgc ctcgtccccc
    17581 gtggccgcgg cgagcggctc gaacttccct tcacgaagcg gcagtccgag ccggaccgcc
    17641 tccgccgcgg cccgcgccag gtagtggcgc gctccgaggt cggcgaacac cgtgtccggg
    17701 gctccgaccc gcgagtcctt ccaccggccg ctctcggtcc ccagcctggc caggatcagc
    17761 gcgagctgtc cgcgcgtcag cggatcgtcc agatggagcg agccgtccgg gaacgcctcg
    17821 atccagtgcc gcaccacgga ggcgcgaagc caccgggcgc cccaggcctg atccagctcc
    17881 ggccagttga gccgctggac ctcgtccgac cgccaggtgg cgcggtcggg atcggcgccc
    17941 agctccaccg cgaggatcac ccccagctcg ccgcgcgaga ccgtgggatg gccgcccagc
    18001 ttggtgtagt cccccgggat tccggccgcc acgtagctcc cctcgagcag tccggcgagc
    18061 gcttcgcgcg cgcgggcgtg ccccggatcc agctccaggg cccgggcgaa ggaggagcgc
    18121 gcggcctcct tttgcccgtc cgcgagatag gcaacgccga ggaggtagga gggctccggg
    18181 cttcgaggat cgagggcggc cgcccggtcc agctccgact tggcgtcccg atagcggcgg
    18241 atggcggtga agaatcggcc ccgggcgagg tggaccggcg cgctctcggg tgccaaggcg
    18301 agcgcgcggt ccagcgcgcg ccgcgccgtg gcggcgtccg cggccgcgat cgcggtgtcg
    18361 gcctcggcga tcagcgcggc gggatcggac gacgtgccgg aggcgggatg gacggagggc
    18421 ccgcgcgcgc cggagcagcc ggccgcgagc agcgccacaa cggcgaccca gcgcgccgcc
    18481 cgctcagcca cgcaggagcc tccgctcctt gaagctggcg tagctctccc gcgcgacgac
    18541 cacgtgatcg aggaggtcga tgcccaggat ccgccccgct tcgagcaggc gccgcgtgat
    18601 ggccagatcc tcctcgctgg gggtcggatc ccccgagggg tggttgtgcg cgagcaccac
    18661 gctcgccgcg gacatcgcga cggccggcag gtagacctcg cgcggatgga cgatgctcgc
    18721 gttcaggctc ccgatcgaga tgatctcgcg ccggaggacc tggtttcggg agttcaggta
    18781 gaagcccacg aagtgctccc ggttcatcgc gcggatgtcg cgcgtcagct cgtagacgct
    18841 ctcgggaccg cgcacgagcg gcgtcgtgtc gccgtcgggc cagagcctgc ggcccagctc
    18901 ggccgccgcg agcagctggg cggcccgcgc acgccccacg ccgcggatcg tgagcaggtc
    18961 ctccggcgcg gcctggtgca gccgccgcac gttgaccgcc ccggtcaccc gggccgccgc
    19021 cgcgagcgcc ggcgtgcccg cgctccccgt tcccaacagc accgcgatca gctcggcgtc
    19081 ggtgaggacg ccggcgccca gctcgagcag acgctcgcgc gggcgatccg ctgggtcgat
    19141 ctcgtgaagt tccagacggc gctccggctc caaggcttcc tcctgacgag ggagccgcgg
    19201 cacgacgaag ctcgaggagt catgatccta gaccggatca gccggcggcg agaagcgact
    19261 tcagatcctt ccggtacagc acgacctggg cgcgggagcg gagccgcgcg gtccaggcct
    19321 cgatgcggcg gtccacgatg tcccgccaca cctcgtcgcg gtggcgcgcg aagtccaccg
    19381 cgggcggcac ggtgacgctc tccacgatca tgtaaatggt ccccatcgag ctcttcacgg
    19441 gagggagcac cttgccgggg cggctcgtga agatccgttc caggagcacc gagtcgctcg
    19501 ccacgcgggg atcgcggtcc ttgaagtcgg ggatcgggcc ggagcgcgcg aacgagcggc
    19561 tcatccgcat cccgcccagc ccgtcgaaga gcgcctcgac gttctcgccg tgcgcgacgg
    19621 cctcgcgaag ccgctggtcc accgagtcgg ccagggcgcg ccgcagcgac atctggtatt
    19681 cgccgatcac ccgctccttg atctccgaaa acggcgccgg ggcgggctcg acctcgcgca
    19741 cgcgccgagc gaccagatat ccctcgccca cctggaccgg ctccggcgtc gtggcgccgt
    19801 cggcgatgtc gccgatcgcc gtggccacgg tctcgaaggg accgatcccg ggcagcgcct
    19861 cgccggcctg ggtgggtccg ctgcgcaccg cgccgccgtg cgcggcggcc agggaatcga
    19921 acgaggctcc cgcttcgagc gagtcgatga atgccgacgc cgcgcggaaa gcgagcgaat
    19981 cggccacggg ctgtcccagc acggtctgga tctcggagcg cgcctgggac aacggctgga
    20041 gcgtgtcgcc gacgcgctcg tccaccttga tcagatggaa ggcgctccgc gcctccacca
    20101 cgtcgctcac ctggccgacc gggatcgtga agatcacgtc ggccagctcc ttcatgaggc
    20161 ggctcttggt cacgtccccg atgagccctc cctgctcccc gctctcccgg tcgtccgaga
    20221 cctccttggc gaccgcggcg aagtcctccc ccttcgcgat ccggtcgtgc gccgcgttcg
    20281 cccgctggag cgccttgtcg cgcgcctcgg gaccgtcgga cggccggtac tggaagagga
    20341 tgatccgggg cttcgccgtc gccggcgtgg tgaactcgag cgggtgctcg ttgtagtagg
    20401 ccgcgatcgc gctgtcgggg accggaacgc tgtcggggtg cgcccgcttg aagtagacgt
    20461 aatcgaagat ccactccggc ttcgtcttga agtcctcctt gtgggcctcg tagtaggtct
    20521 ccgcgtcctt ctccagcgcc cagcgcttga ccatctgcgc gttcgagctg gccgcctggc
    20581 ggaccgcctc gtacggcggc acgaacccgg gatcgatccg gtccacgcgc accacgaaca
    20641 gggagtcgtg cgcggcccga gggccgacca ccgcgccggg ccgcatcgcg tagagggaat
    20701 cgaggaacga gccctcgagc aggatcggct cttcgatcgg ctggccgcgg tagaggtcca
    20761 ccgattgcgc gaccccgccc gtttcgttcg ccacccgcgc gccgggagcg ccgcctcgga
    20821 gacggaccac cgtctcgcgg aaggcgcgct ggatcgcctc gtcgcgccgc tgctttcgca
    20881 cctcggccgg gagggccgcg gccagcgagt cgagccacgc gcggttcgac ttcttgatct
    20941 tggcctcctt ccgccgcctt tcgaggagct tctggatctc cttggcggtg ggagcggcgc
    21001 cggtatcgat ctcggccgac gagcgcgcga tcaccgtggc ggccagacgg ggcacggcgt
    21061 acgtctgcgg atggaggcgc agctcctccc ggccgagcga gtccgccagc ccctcgcgga
    21121 tctgcgcgtc ggcccgccgt ttcgcctggc cggcgacctc gcggaagctc tggatgcgcg
    21181 cggcacgacg gtccaccagg cggatcacga cgtagtgggc gcccgcccgg atcggcgtct
    21241 tgacccacgt gccgggagcg gcgccgcgga tggcgtcctg gatcgcctcg gagcgtccga
    21301 gcccgcgaat cggatcggag aggcggaacc atcccgtgtc gtgaagcccg ccgtagacct
    21361 tcgccgccgt ctcgaccggc gcgcccgact gcatcgcacc gaccagatcg ctcgcggcct
    21421 tctgggcgct ggcgacggcg gcatctttcg ccgaatcgcc ggaagcggcc tcgatcggaa
    21481 cccgcacgta ctggatccag gcggaatcgg gagactggaa ctcctgctgg tgcgcgtcgt
    21541 agtaggcgcg gatctgcttc gcttcggcct cgggctccag cgacacggcc tcaggtccca
    21601 gcacgaagta gcgtaccgag gcctggctgg ccaggtcctg gtaggccttc cgcacttcgg
    21661 gctcgcgcgg tccgaagcgc cgctccatcc accgcgcgta gtcctcgaga aggatgccca
    21721 tcgccacctg gtcgcgcagc agcgcgtagt tcgacgtcgg cgagcgcttg aaggcgagga
    21781 acttcgattc gtccaccttc cccttcacct tgaagaaggc gttctccttc atgcgagcgt
    21841 ccacgacgtc ctcgttcacc cgcatcccgc ggcgatgggc gtcggccagc cacagccgtt
    21901 cgcggatcag ctcgtcgagc aggttcttgc ggaggagacg cttctcgtcg tcgctcagct
    21961 tccgtccggc cttggcctcg acctcctgga agtagggggc ggccagacga tcccactcgc
    22021 tctgcatgat ggggacgcca tccacgacgc cgatgacgtc gggcgtcggc ttggcggacg
    22081 ggctcgcggc ggggacgcgc ggaggcaccg cgggcgccga gaaggcggcg gccgcgacgg
    22141 ccagcaccga tacggcgagc agcgtcggac ggaggcgcgc gaatccggaa ggaatggagc
    22201 agttgttcac gggagattaa gtctagaaac cgcggagggg cgtgtccacg agagaaacga
    22261 aatcaggccc tcggcgggcg gctctcgcga agtgtggggg gcttatcgtt cgcgagtctg
    22321 ccgtcacaac cgagggcctg tttaccctct cggtcccccg gaaccacgct cgatcggagt
    22381 gccgtgcctt cccacgcgcc ggcagctctc cccttcaccg ctttcctggc cttcgcgagc
    22441 gttcttgcgt gactcgcgag gcgaggaccc ttgccccaac acgtcgagct tcggtccgag
    22501 ggcccgagag agtccaagac ctccctggcg gctcggccta tggcacccac cgtgccatct
    22561 tgagatcgcc tcggttgccg cggtgcaggc atggcggtaa ccatattgtt atcaaaggtt
    22621 aacaggtcaa atgtgtcaaa ttagccacgt tggaatgaat agaccgatcc accacggctg
    22681 tgtgtcgatg tggctcattt gacggaagga tcgaggcgta gatactttcg aatcaataaa
    22741 ttactggact gtgagtcact gagacacaga cgggacgtct cgtctcagtt ttgacttgcg
    22801 ggagcgtacc gctccgtagc gttccagcat gcggtaaagg gtccttcgag gtactccgag
    22861 cacccgcgcc gcctccgttt tgttgccccc cacctcctcc aatacgtgta gcacgtactc
    22921 ctgaatcgtc tcgtccaggg tggctcgccg gacctccccg cgcccgccct ggctctcccg
    22981 cgtgccgatg cgggcgtgga cgatcagctt atcggggaga tcggtcagct cgacgatcga
    23041 ccccggcgcg agcgcgaccg cgcggtcgac cacgttctcc agctccctca cgttgcccgg
    23101 ccaggcgtaa gcctggagcg cggtcatggc cgaagcggaa aaccccagga gcggccggtt
    23161 ggagagcgcg ccgaagcggc ggaggaaatg ctcggccagg agcggaatgt cctcggggcg
    23221 ctcggccagc ggcggcacac gcagggtgac cacgttcagc cggtagtaga ggtcctcgcg
    23281 gaaccgcccg gcgcgcacga gcgcgtccag gtcctgatgc gtcgccgtca ccatccgcac
    23341 gtcgaccgga atcgtctcgt tcgcacccac cggcttcagc tcgtgctcct gcagcacgcg
    23401 aagcagcttc gcctgcatcg ggagcgacat gtccccgatc tcgtccagga agatcgtgcc
    23461 gtggttcgcc tcgacgaaga ggcccgggcg gcgctcgatc gcccccgtga aggcgccttt
    23521 gacatgtccg aagagctccg attcgagaag cgtctcggag aggctggtca cgttcaccgc
    23581 gacgaagggg cgggaggacc gcggacttcc ctggtggatc gtccgcgcga tcagctcctt
    23641 tccggtgccg ctctccccct ggatcaacac gctcgacttg gtcgcggcca cgcgtgagac
    23701 cagcttgaac agctccacca tggcgggatt ccgcccgatg atcgggcgcg cctccgtgct
    23761 ctcggcggag gccgcgcccg cggccgcggc actcctctgg agccggcggc gctccatggc
    23821 gcgctccacg gtgaggcgca cttcctgaag cgtgaacggc tttcgcacgt agtcgaaggc
    23881 gccttcgtga agcgccgtga tggcggtctc gtagctgccg aaggcggtca ccatgatcac
    23941 ggcggcgccc ggcatggcgc gccggatgcg gcgcaggagc tcgatgccgt ccatgtcggg
    24001 aagctgcacg tcgcagagcg cgacgtcgaa ggggcgggcg ggaacgaggg cgagcgcttc
    24061 ttccccacgg gccgcgaggg tcacctcgta gccttcctgc tgaaagatgt cgcggaggag
    24121 ccccgaggag ccggggtcgt catccacgac gagcatggtg ggcctgtgtc gcgccatgag
    24181 gccgcgtcac cgcccttccg tggggcgagc cgccatgcgc cgcattgtat ccgagccgcg
    24241 ccgcgaagga agaaaaacga tgcgggaacg atgcttcggt tgcccgctta cggagccttc
    24301 cactaggatg ggcgccactc gaggacgagc ccggctgccg cgaagcccgg cacccacgga
    24361 ggcgatcgca tggcaccgcc cgattccgat tccgaagtcg ccgagagact ctcggtcgaa
    24421 gacctccggg aggtctggaa tctcctcgtc cccgaggacc ggatcgagag cttccgggcg
    24481 ctgccccgcc ccgaggccga ggatctcttc ctcgagctct ccgcgcggga ccagaccgag
    24541 ttgctcctcg ccctgcccgg gcccgagcgc cgctcctggg tgcgtctcct cccgcccgac
    24601 gacgcggccg acctgatcca ggaggccggc gccgagcacc gcgaatcgct cctccagctc
    24661 ctcgacgatc ccacgcggaa ggacgtccac gcgctcctcg cctacgccga ggacgacgcg
    24721 ggcggattga tgaatccccg ctacgcgcgc gtgcggcccg agatgagcgc cgacgaggct
    24781 ctgacctatc tccgccggca ggccagcgag cagaccgtgt cgctgcccta cgcctacgtg
    24841 ctcggaccgg atcagaagct gatgggcgtc gtctcctacc gcgagctctt cctcgcgcgt
    24901 cccgagcagc gcgtgcagga cgtcatgcgc accgatctcg tgaccgcgcc ggaatcgatg
    24961 gatcaggagg agctcagccg gctcttcacc gagagccgcc tcatcgcgat ccccgtcatc
    25021 gacgccgagg gacggatgaa ggggatcgtc accctcgacg acatcgtcga cgtggtccgc
    25081 gaagaggcca ccgaggacat ccagaagatc ggcggtaccg cggccttcga cgagccctac
    25141 ctgcgcctca gcgtgagaga gatgatcatc aagcgggccg ggtggctctc ggtcctcttc
    25201 ctcggcgaga gcatgaccgc ggccacgatg gggtacttcg agcaggagat cgctcgcgcg
    25261 gtggtgctcg cgctcttcgt ccccctggtc atctcgagcg gcggcaactc cggcggccag
    25321 gcgaccaccc tggtgatccg cgccatgtcg ctcggcgagg tccgcctccg cgactggtgg
    25381 cacgtcatgc gtcgcgagat cttgtcgggg ctgggccttg gcaccattct cgccgtgatc
    25441 gggatcgtgc gaatcatggt ctggcaggcg atcttccact cgtacggcga gcaccacctg
    25501 ctgatcgcgc tgaccgtcgc cgtgagcctg gtcggcgtcg tgatgtgggg aaccctggtg
    25561 ggttcgatgc tccccttcat cctccgccgc ctgggcctcg accccgcgac cgcgtccgca
    25621 cccttcgtcg ccacgctcgt cgacgtgacc ggactgatca tctatttctc ggcggcgcgg
    25681 gtgttcctgg gtggcgtgct gctgtagtcg gactacggaa cgaagacggt ttctcccgcc
    25741 tgttcctcgg cgtggaacga cgatcggacg aggggaccgg acgcgacctg gcggatgccg
    25801 agggacaggc cgtactcgcg gtagtggcgg aactcgtcgg ggtggacgta gcgctccagc
    25861 ggaaggtgcg agcccgacgg cgggaggtac tgccccacgg tgaccatgga gacgccgtgg
    25921 tcgcgcagat cgcgcagcag ttcatccact tcgtcctgcg tttcgccgac tccaaccatc
    25981 acccccgact tcgcggggat atccggcgtc caggtcgcca cgttcttcaa cagctgcagc
    26041 gaccgctcgt agcggcccgc ggggcggacc cgcttgtaca gccgcttcac cgtctccata
    26101 ttgtgattca ggacgtcggg cttggcgtcg agcacggtgc ggagcgcgtc ttcgtccccc
    26161 aggaaatcgg ggatcagcac ttcgacccgc gcgccgggga tcgccccgtg aatcgcgcgg
    26221 atcgtctcgg cgaagtgcat agcaccgccg tccggcagct cgtcgcgatt caccgacgtg
    26281 accacgatgt agtggaggcc gagccgccgc gacgcgtccg ccacccgcgc cggctcctcg
    26341 ggatcaaccg ggagcggctt tccgttccgg atgtcgcaga aggggcagga gcgcgtgcag
    26401 acgttgccca ggatcaggaa cgtcgccgta tgccgcgaga agcagtcgcc gatgttcggg
    26461 cagttcgcgc tctcgcagac ggtgtgcagg tccagcgtcc ggaggatcga cttggtctcg
    26521 aggtagttcc ggcccgtcgg gatgcgcgcg cggagccagt ccgggcggcg cacgggaagc
    26581 ggatccccgc tctggcgctc gtagctccgg agcggcttcc gtttctcgcc ggcctgaaca
    26641 cggcggtcgg acgggagcgt gagcgagatg gccgtgcccg agatgggatg gcgcggcacg
    26701 tccgaagcgg ccgcgcgatc ccgcgcggcc ccgtccggtc ccgtccccgt gcgctcggca
    26761 tcagacatgg atcgctcgtc cctcggttcc aaacatcgcc tccatcgtcg cctcggccag
    26821 cgtcggatgc gcgtgcacga cattggccag ctcctcggcg gtcgcctcga aatgccgggc
    26881 caccaccgat tccgcgatct gctccgtggc ctggaccccg atgatgtgga cgcccaggat
    26941 ctcgccggtc gccgcgtccg cgaccgcttt cacgaagccg tcgccctctc cgaggatact
    27001 ggcc
//
LOCUS       DASSLZ010000120         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_40067, whole genome shotgun sequence.
ACCESSION   DASSLZ010000120 DASSLZ010000000
VERSION     DASSLZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40067"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFM00_04520"
     CDS             <1..201
                     /locus_tag="VFM00_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET7497542.1"
                     /translation="DPYVEQNSYVDVGQTVCIVEAMKLMNEIESDVKGRIARILVENA
                     QPVEYGQILFLVETDGSAERAA"
     gene            210..1328
                     /locus_tag="VFM00_04525"
     CDS             210..1328
                     /locus_tag="VFM00_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET7497543.1"
                     /translation="MNIKRVLQQMVEMKASDLHVKVGTKPTARVHGLLVPIDEPAPSQ
                     ADLEAVVDQILTPKQKKVFEETKEVDFAFGVTGLARFRTNFYQQRGTTAMVFRQVPAT
                     VPAFEELNLPPVIEELAARPRGLVLVCGTVGSGKSTTLAAMLDSLNRREARNVITIED
                     PVEFLFRDKKCTISQREIGLDTATFAEGLRHILRQDPDAIMIGEIRDAETMRIALMAA
                     DTGHLVLSTLHTVDATQTVNRIISFFPPHQHEEIRFMLAGTLQAVIALRLVPRSDLEG
                     RIPAAEVMLVTATIREYLQDPDKTSLIKAAIAEGVSQYGMQTFDQSIMKHYTEGKVSL
                     ENALRYCSNPTEFELRVKGIHATSDESWKSFERKEEVV"
     gene            1335..2696
                     /gene="accC"
                     /locus_tag="VFM00_04530"
     CDS             1335..2696
                     /gene="accC"
                     /locus_tag="VFM00_04530"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET7497544.1"
                     /translation="MFKKILIANRGEIALRIIRAARELGIETVAVYSEADRDSLHVRL
                     ADEAVCIGPPPSAKSYLNIPRIISAAEVTAADAIHPGYGFLSENAHFAEICETCKIRF
                     IGPTSEMIRKMGDKAEARRTMMAAGVPITPGSEGVIDSVDEAERVAKELGFPVIIKAA
                     AGGGGKGMRIATDEASLQNGIRMAQAEAEANFGSGSVYLERYVQRPRHIEVQVLGDRH
                     GSIVHLGERECSIQRRHQKLIEESPSPALTPELRAKMGQAAVKGAASIRYEGAGTIEF
                     LLGPSGEFYFMEMNTRIQVEHPVTEEVTRLDLIKAQIAVAAGDPLPWKQEEIRMEGHA
                     LECRINAERPDQNFQPSPGLVRYFHAPGGPGVRVDSHLYSGYTVPPHYDSMVAKIICW
                     GRDRAESVARMRRALEETVVDGIDTTLAFHIRVLDDENFRKGEIHTGFIEEFLTRERA
                     AAA"
BASE COUNT          514 a          945 c          961 g          397 t
ORIGIN      
        1 gatccctacg tcgagcagaa cagctacgtg gacgtggggc agacggtctg catcgtggaa
       61 gcgatgaagc tcatgaacga gatcgaatcg gacgtgaagg ggcggatcgc ccgcatcctg
      121 gtcgagaacg cccagccggt cgaatacggg cagatcctct tcctcgtgga aaccgacggg
      181 tccgccgagc gggcggccta ggagaatcga tgaacatcaa gcgcgtcctg cagcagatgg
      241 tggagatgaa ggcgtccgac ctccacgtga aggtgggcac caagcccacc gcccgcgtgc
      301 atggtctgct cgtcccgatc gacgagccgg ccccctcgca ggccgacctc gaggcggtcg
      361 tggaccagat cctcaccccg aagcagaaga aggtcttcga ggagacgaaa gaggtcgact
      421 tcgccttcgg cgtcaccggg ctcgcgcgtt tccgcacgaa cttctatcag cagcgcggca
      481 cgacggcgat ggtcttccgg caggtgccgg ccacggtgcc ggcgttcgag gagctgaacc
      541 tcccgcccgt catcgaagag ctggccgccc gcccccgcgg gctggtcctg gtctgcggca
      601 ccgtcggcag cggcaagtcg accacgctcg cggcgatgct cgactcgctg aaccgccgcg
      661 aggcgcggaa cgtgatcacc atcgaggacc cggtcgagtt cctcttccgc gacaagaagt
      721 gcacgatcag ccagcgcgag atcggtctcg acaccgcgac gttcgccgag gggctgcggc
      781 atatcctgcg ccaggacccg gacgccatca tgatcggcga gatccgggac gccgagacga
      841 tgcgcatcgc gctcatggcc gcggacaccg ggcacctcgt cctctccacg ctgcacaccg
      901 tggacgccac gcagaccgtg aaccgcatca tctccttctt cccgccgcac cagcacgagg
      961 agatccgctt catgctggcg ggaacgctcc aggcggtgat cgcgctgcgc ctggtgccgc
     1021 gctccgacct cgagggccgg attcccgcgg ccgaggtgat gctggtcacg gccaccatcc
     1081 gcgagtacct gcaggacccc gacaagacct cgctcatcaa ggcggccatc gccgagggcg
     1141 tctcccagta cgggatgcag acgttcgacc agtcgatcat gaagcactac accgagggaa
     1201 aggtgtcgct ggagaacgcg ctgcgctact gctccaatcc caccgagttc gagctgcgcg
     1261 tgaagggcat ccacgccacg tcggacgaga gctggaagag cttcgagcgg aaggaagaag
     1321 tcgtctagcg ccccatgttc aagaagatcc tgatcgcgaa ccgcggcgag atcgcgctgc
     1381 ggatcatccg cgcggcccgc gagctgggga tcgagacggt ggccgtctat tccgaggccg
     1441 accgcgactc cctccacgtg cggctggccg acgaggcggt ctgcatcggc ccgcccccct
     1501 cggccaagag ctatctcaac atcccgcgca tcatcagcgc cgccgaagtc acggccgccg
     1561 acgccatcca ccccggctac ggcttcctct ccgagaacgc gcacttcgcg gagatctgcg
     1621 agacctgcaa gatccgcttc atcggcccca cgtcggagat gatccggaag atgggggaca
     1681 aggcggaggc gcggcgcacg atgatggccg cgggcgtgcc gatcacgccg gggagcgaag
     1741 gggtgatcga ctccgtggac gaggcggagc gcgtcgccaa ggagctgggc ttcccggtca
     1801 tcatcaaggc cgcggccggc ggcggcggga aagggatgcg gatcgccacg gacgaggcgt
     1861 cgctccagaa cgggatccgc atggcccagg ccgaagcgga ggcgaacttc gggtcgggct
     1921 cggtctacct ggagcgctac gtccagaggc cgcggcacat cgaggtgcag gtgctgggcg
     1981 accggcacgg ctcgatcgtg cacctggggg agcgcgagtg ctccatccag cggcgccatc
     2041 agaagctgat cgaggaatcg cccagccccg cgctcacgcc ggagctgcgc gcgaagatgg
     2101 ggcaggccgc cgtcaagggc gccgcctcga tccgctacga gggggccggc acgatcgagt
     2161 tcctgctcgg cccctcgggc gagttctatt tcatggagat gaacacgcgg atccaggtgg
     2221 agcaccccgt gaccgaagag gtgacgcggc tggacctgat caaggcgcag atcgccgtgg
     2281 ccgccgggga cccgctcccc tggaagcagg aggagatccg gatggaggga cacgcgctcg
     2341 agtgccggat caacgccgag cgtcccgacc agaacttcca gccctcgccg ggcctggtcc
     2401 gctacttcca tgcgccgggt ggccccggcg tgcgggtgga cagccatctc tattcgggct
     2461 acacggtgcc gccccactac gactcgatgg tcgcgaagat catctgctgg ggacgggacc
     2521 gggcggagtc ggtcgcgcgg atgcgccgcg cgctggagga gacggtggtg gacggcatcg
     2581 acaccacgct cgcgttccac atccgcgtgc tggacgacga gaacttccgc aagggagaga
     2641 tccacaccgg cttcatcgag gaattcctca cgcgcgagcg ggcggccgcg gcgtgagtcc
     2701 ccgcacgtcg aaggtcgagg ccggcgcgaa cgagggcggc ggtctgaaac ggatcgacgt
     2761 cttcttcacg ccctccgcag cgtccgagcg atcgcacctg gaaggacgct cgatcgt
//
LOCUS       DASSLZ010000121         3699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_40934, whole genome shotgun sequence.
ACCESSION   DASSLZ010000121 DASSLZ010000000
VERSION     DASSLZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40934"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2818
                     /locus_tag="VFM00_04535"
     CDS             <1..2818
                     /locus_tag="VFM00_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7497545.1"
                     /translation="ALVLWGRDRSREDDSENAIRAALAMRDRVLAESRKVLGAAWEPT
                     EENPLPFLAGITTGPVLLERESDSGSYTASGAPITLAGRLKDAAPPGGILVAHETFTL
                     VRGVFTFTQREPLRVRGRKEPIEVYLVTQVKPRAFRLRARGIEGVETKMIGREIELRL
                     LQEALSLTLEDGETQVVTVVGEAGVGKSRLLFEFSSWIDLQETTVWFFQARATQPSML
                     QPYSLARDLFSFRFRILDSDPLSVVHEKFVKGIEEFLGQGSERKAHFIGQLVGFDFSA
                     MPDVESALKEGDAFRREAQGYLGELFTTASKVHPVMIHIEDIHWADDRSLDLVNNLVR
                     DNTDLPLFVLCMARPSLYERRPQWGEGQRFHERIQLEPLSQLSSRRLVRELLKNVPEV
                     PTALRDLVVDRADGNPFYIEELIKALIDDRVILKGEESWSVDTTRLSSVRIPATLTGV
                     LQARLDTLPAPLHQLLQRASVVGRVFWDAAAVHLSRETAGQKPAEVQEMLEELRGREM
                     ILRREESGFAGTVEYVFRHAIFRDVTYETIIPRQRRALHKLVGDWLIEIGGERAGEHT
                     LLVAEHYARADEPSLAAGQLMKAGERGLLLATFEEATTLLERARGLLAGPEHIEQRLE
                     VDMLRGEVEAYRAHYPAASEIFQSALADARSTKNARIQARALGQLGRIAMWQGDVSTA
                     LQRIGEALEASRAAGDRESLIFNLRQMGNMTVQRDPERAIEYQQESVDLARKAGDSNA
                     VAAGLNSLGIAFNLSGRTEDSRRAFTEALELNRAIGDRSGHAMVLSNLAGLHAGAGDY
                     DNAEQTARESIRIAREIGTVGMVPAGQAVLVEVALRRGRAAEARALLREFATTLRALG
                     VPLYPVAFFYGVLELLTGERARGLAWIGYVRANDPNERELALAIDAFRDLIRGGAPDA
                     EVEAAMKAGESLTLEEILAEAERE"
     gene            complement(2850..3287)
                     /locus_tag="VFM00_04540"
     CDS             complement(2850..3287)
                     /locus_tag="VFM00_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497546.1"
                     /translation="MKNTIERSGRAQAPYREQSEYDDAEVDNGRAPGERMANALGWFS
                     LGLGAVETTAPGAVAKFLGIPRRDRVIRAMGMREITAGIGILTKGQPVRWMWARVVGD
                     VIDLAMLGAAVPASERRDRVAWSAGFVAGILAVDVVCARQLAL"
     gene            complement(3368..>3699)
                     /locus_tag="VFM00_04545"
     CDS             complement(3368..>3699)
                     /locus_tag="VFM00_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497547.1"
                     /translation="IHPCEGPGAEMRMGTKLLRTFVAAGLPEPQFLVEAPAGGGSDWP
                     GYEYLADTLRSLMPMLQQRAGVDPDEVQVDTLAGRLREDVARVQGIHILPIMFGAWTR
                     TRSSRME"
BASE COUNT          584 a         1260 c         1280 g          575 t
ORIGIN      
        1 ggcgctggtc ctctggggcc gggaccggtc ccgcgaggac gactcggaga acgccattcg
       61 cgccgccctg gcgatgcggg atcgcgtcct cgccgagtcg cgcaaggtgc tgggggctgc
      121 gtgggagccc accgaagaga acccgctgcc ctttctggcc ggcatcacga ccggccccgt
      181 tctcctggag cgcgagtcgg acagcgggag ctacaccgcg agcggcgcgc cgatcaccct
      241 ggcgggccgt ctcaaggacg cggcacctcc cggcggaatc ctcgtcgcgc acgaaacctt
      301 cacgctcgtg cgcggcgtct tcacgttcac gcagcgggag ccgctgcgcg ttcgcgggcg
      361 gaaggagccg atcgaggtct acctcgtcac ccaggtgaag ccgcgcgcgt tccgcctgcg
      421 ggcccggggc atcgagggcg tcgagacgaa gatgatcggg cgcgagatcg agctgcgcct
      481 gctgcaggaa gcgctgtcct tgaccctgga ggacggcgag acgcaggtcg tcacggtggt
      541 gggcgaggcg ggcgtgggaa agtcccggct gctcttcgaa ttcagcagct ggatcgacct
      601 gcaggagacg acggtctggt tcttccaggc gcgcgccacg cagccctcga tgcttcagcc
      661 ctactcgctc gcgcgggacc tcttctcctt ccggttccgc atcctcgact ccgatcctct
      721 ctccgtggtg cacgagaagt tcgtgaaggg gatcgaggag ttcctgggtc agggctccga
      781 gcggaaggcc cactttatcg ggcagctcgt cgggttcgac ttctcggcca tgcccgacgt
      841 cgagtcggcg ctgaaggaag gggacgcgtt ccgaagggaa gcgcagggct acctgggcga
      901 gctcttcacc acggcatcca aggtgcaccc ggtgatgatc cacatcgagg acatccactg
      961 ggcggacgac cgttccctgg acctcgtgaa caatctcgtt cgcgacaaca ccgatcttcc
     1021 gctcttcgtg ctctgcatgg cgcgccccag cctctacgag cgccgcccgc agtggggcga
     1081 ggggcagcgg ttccacgagc ggatccagct ggagccgctc tcgcagctct ccagccggcg
     1141 cctggttcga gagcttctca agaatgtccc ggaagtgcct acggcgcttc gggatctagt
     1201 ggtcgatcgt gccgacggca accccttcta catcgaggag ctgatcaagg cgctgatcga
     1261 cgaccgcgtg attctgaaag gggaggagag ctggtcggtc gacacgacgc ggctctcgtc
     1321 ggtgcgcatc cccgccacgt tgaccggcgt cctccaggcg cgcctggaca cgcttccggc
     1381 accccttcac cagctcctgc agcgcgcctc cgtggtcggt cgcgtgttct gggacgcggc
     1441 cgccgtccat ctcagccgcg agacggcggg acagaagccg gccgaggtgc aggagatgct
     1501 ggaagagctg cgcggccgcg agatgatcct gcgccgcgag gagtccggat tcgccgggac
     1561 tgtcgaatac gtgttccggc acgccatctt ccgcgacgtc acctacgaga cgatcatccc
     1621 ccggcagcgg cgggcgctgc acaagctggt gggcgattgg ctgatcgaga tcggcggcga
     1681 gcgtgccggg gagcacacgc tgctcgtggc cgagcactac gcccgcgcgg atgagccctc
     1741 actggccgcc ggccagctga tgaaggcggg ggagcgcggg ctgttgctcg cgacgttcga
     1801 ggaagcgacg acgcttctgg aacgggcgcg gggacttctc gccggccccg agcacatcga
     1861 acagcggctc gaggtcgaca tgctgcgcgg cgaggtggag gcctaccgcg cgcattaccc
     1921 tgcggcctcg gagatcttcc agtccgcgct tgccgacgcc cggagcacga agaatgcgcg
     1981 gatccaggcc cgtgcgctgg gtcagctggg gcggatcgcc atgtggcagg gggacgtctc
     2041 gaccgcgctc cagcgcatcg gagaggcgct cgaggcctcg cgcgcggcag gcgaccggga
     2101 atcgctgatc ttcaacctcc gccagatggg aaacatgacc gtccagaggg atccggaacg
     2161 cgcgatcgag taccagcagg agagcgtgga tctggcccgc aaggccggcg attcgaacgc
     2221 cgtggccgcc gggctgaact ccctgggaat cgcgttcaat ctcagcgggc gcacggagga
     2281 ctcccggcga gccttcacgg aggcgctcga gctcaaccgc gccatcggtg atcgcagcgg
     2341 ccacgccatg gtcctcagca atctggcggg tctgcacgcg ggcgcgggcg actacgacaa
     2401 tgccgagcag acggccaggg aatcgatccg gatcgcgcgc gagatcggga ccgtcgggat
     2461 ggttccggcg gggcaagcgg tgctcgtcga ggtggccctg cggcgcgggc gggctgccga
     2521 ggcacgggcg ttgctgcgcg agttcgcgac gaccctgcgc gcgctcggag ttccgctgta
     2581 tccagtggcg ttcttctacg gggtcctgga gctcttgacc ggggagcggg cgcgagggct
     2641 ggcctggatc ggttacgttc gggccaacga tccgaacgaa cgggagctcg cgttggccat
     2701 cgatgccttc cgcgatctca tccgcggcgg cgccccagat gcggaagtgg aggccgcgat
     2761 gaaagcgggc gagagcctga cgctcgagga aatcctcgcc gaggcggagc gggagtagcg
     2821 ccagcccctc gccctcggtc caaccggttc tacaaagcga gctgcctggc gcacacgacg
     2881 tcgaccgcca ggatgccggc cacgaacccc gccgaccacg ccacccggtc gcgccgctcc
     2941 gaggcaggca ccgccgcgcc gagcatcgcc aagtcgatga cgtcgcccac cacccgcgcc
     3001 cacatccacc gcaccggctg acccttcgtg agaatgccga tcccggccgt gatctcgcgc
     3061 atgcccatgg cccggatcac gcggtcccgg cggggaatgc ccaggaactt cgcgaccgct
     3121 cctggagccg tggtctcgac cgctccgagc ccgaggctga accagccgag cgcgttggcc
     3181 atccgctcgc cgggagccct cccgttgtcg acctccgcat cgtcgtattc gctctgttca
     3241 cggtagggcg cctgcgcgcg gccgctccgc tcgatggtgt tcttcatgac gatctccttt
     3301 ctgttgttcc cggcgcgagc acgaaactct ctctcgcgtc ccgaagaccg tccggaaggt
     3361 gcaatgatca ttccattcgt gaagatcggg tgcgcgtcca ggccccgaac atgatcggca
     3421 ggatgtgaat cccctggacg cgcgcgacgt cctcgcgaag acgacccgcg agcgtgtcca
     3481 cctggacctc atccgggtcg acgccggccc gctgctgcag catgggcatc aggctgcgca
     3541 gggtgtcggc caggtactcg tagcccggcc agtcgctgcc gccgcccgcg ggcgcctcca
     3601 ccaggaactg aggctccggg agacccgcgg caacgaaggt tcggagcagc ttggtgccca
     3661 ttcgcatctc ggctccaggg ccctcgcagg gatggatgc
//
LOCUS       DASSLZ010000122         9517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_159192, whole genome shotgun sequence.
ACCESSION   DASSLZ010000122 DASSLZ010000000
VERSION     DASSLZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9517
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159192"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFM00_04550"
     CDS             complement(<1..322)
                     /locus_tag="VFM00_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497548.1"
                     /translation="MNLRRQITELVKLMREEDLAEVEVRRWFSTIRIRRPGGEMVAAS
                     APAREAHTAAPPAAAEAAPDESNREQALSMLAEALRATGNTREAAGLHREILARLDSS
                     GFLGA"
     gene            complement(319..792)
                     /locus_tag="VFM00_04555"
     CDS             complement(319..792)
                     /locus_tag="VFM00_04555"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET7497549.1"
                     /translation="MAGTRDVWVLHGPNLGLLGRREPELYGKNALAAIDKRLAALGEE
                     LNLNVTSFQTNHEGALIDRLTIAMDEADGCLLNPAAFTHTSLALADALRSVTIPVVEV
                     HLTNLYARERERQTSLTGSAVRGVIMGLGAMSYELGLRALDALLRAGKRGRGYGR"
     gene            complement(792..1679)
                     /locus_tag="VFM00_04560"
     CDS             complement(792..1679)
                     /locus_tag="VFM00_04560"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497550.1"
                     /translation="MVERDPSTALYLPLAERLREEGRVEEAIRLCEARQNRPGHGVGD
                     AIVLGRCYLADGRLNEARSSFEAALALDRENVSALKALAGILAHEGRHARSVELYRAV
                     CRVDPGDLESQTALQQITSGEYADVCPADVVVGQGDLGWHPVRLPREEEHLSALGLGL
                     RTIETFGEESAASGSDRDRARSFQELSLDAPAMPERPMATPAPPASRAPASDVASSGI
                     APSDLPPSGIAPSDVSPPAPDLALPEPDAPPRPAFSSPGSSAGRAPSAPSTPAPPDEP
                     VRGNRSAFEDWLRRLGGGA"
     gene            complement(1738..2883)
                     /gene="aroB"
                     /locus_tag="VFM00_04565"
     CDS             complement(1738..2883)
                     /gene="aroB"
                     /locus_tag="VFM00_04565"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET7497551.1"
                     /translation="MRGAASSYVVRVERGLLARAGACVRAALEEPRGRARRARGGRAG
                     ARGRSVALVTDRTVARLYGARVEASLRRAGFRVVRTTIAPGEGSKRFETVQVLAEGWA
                     RAGLTREAVVVALGGGVVSDTAGFAAAAYTRGLPWIAFPTTTLAQADAAIGGKTGVNL
                     AAGKNLLGAFHQPRAVFADPEALATLPGRAFRSGLAEVVKMGMIRRPAILRRLDAIAE
                     SLAGKRAARDAAAASLAPILRECAATKAWYVEQDERDTGLRRELNFGHTVGHSLEAAG
                     GYRLYQHGEAVAVGMVAALRMSVLHAGLDPVDAQETERLIARLGLPTRLPRFPGERFW
                     NALARDKKRGRTRLRVVLCPAIGSAEIFELSSLTSLRRVVESLVMRS"
     gene            complement(2993..4210)
                     /locus_tag="VFM00_04570"
     CDS             complement(2993..4210)
                     /locus_tag="VFM00_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7497552.1"
                     /translation="MKLRAAAAGLAILAAPFLGGAAASAAPGPLRPESSPFLGVLDSL
                     QASILTIVAVPEAQHSAPAGVKRKRLIGTGVATNERDIVTTAGLAFPNGDVHVMLGGG
                     VERRAVLRGVDRQSNLAVFRLEEPVLHTLRRAAPQSLAVGTWVAVISNITVSRPQAAI
                     GQVVGRGERVDFPYSGDVIEIDAPGYPASAGGAVVNEDGEWVALVVGRGLQGPNAGES
                     SRVGDPERPGRPPTGGVLLAVPVDQVERIVADLGEHGTVQRGFLGIRLKRGPVSPEDT
                     LGVRVDDVIAGSPAAVAGVRVGDHILAMDGQEVRSAEELVSMVATLRPGYDTEVTILR
                     DAEILPLRIVVGSNITGPRPQGAARSEAPPALNAADKKALREHLLKLREEQRALEERL
                     RGMEASDSLGQNR"
     gene            complement(4236..4949)
                     /locus_tag="VFM00_04575"
     CDS             complement(4236..4949)
                     /locus_tag="VFM00_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7497553.1"
                     /translation="MNCRSAEHLFSSFVEDELSQRERLSLESHLEECARCSAAVQELR
                     VTVDLLSTLPSYETGPAFEEEIMDRIRSGEAMRPGLIEWISEHLAPARLRPVFLAGAA
                     GCALWIAFLLVNPGQDAAPNGGSKIAGTTPKTEQTATSNVPAPSSAVTTPAAPAGDVV
                     ASAPTPARVAPSRTGSVPAALEGTAAASGSADSARINAPYQDEYILDQFYLNRSGEDG
                     VHSIVPVSGRTTDDVYITF"
     gene            complement(4946..5572)
                     /locus_tag="VFM00_04580"
     CDS             complement(4946..5572)
                     /locus_tag="VFM00_04580"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7497554.1"
                     /translation="MPIESLRLKKLDPLAELSDEDVMERCAQGSEAAFRTLVQRYRSR
                     IMNLVCRFINDRDRAEEIAQEVFLRVFRNKERYRKSGKFSTWIFTIAVNLTKNEIRSR
                     VRHRGTYSLDAMDEESGGQGLPFPDWKPLPDADLNAREIQNKVAEALRKIPPRYREAV
                     VLRDVEGLSYEEVGEILRIPGGTVRSRINRARLMLKERLKPYLKLEDV"
     gene            complement(5637..7328)
                     /locus_tag="VFM00_04585"
     CDS             complement(5637..7328)
                     /locus_tag="VFM00_04585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497555.1"
                     /translation="MTTSLRQSLFLLVLGFTAPAFVPPVAHAASRMESGYPSLSGAPP
                     DGVLLDLLTRSELAEAAGDGEAAIQWAESLATADPGSAFGCARAATLLETIGQDSEAL
                     SWGQKALARDSLNLEAAMLVARMHLRGGESDIAAQALTPPLRILGAPPEAYALRALAH
                     ELSRRYDAALADLKRTDELLPDFAWIATGILGLALEDGRLEEASQALQLALELRSDDP
                     RVLHLGVTLAQRTDDPVLEERLLRTLALQSTARPTDIAAYGAFLVRMGKKDSFRQLEA
                     WADAGPMPAADLRAETGEALVREGRYPEALDTVRPLKGDPRAMPVRARAYVSLGEDRK
                     ALECYRTLVPGRTLSREESLVVAYLEIRVGDRARGVETLERARSARLDTPRQVLTASL
                     CYSLLGYPDEAVALIRDSASRGISSPSTFEELGSAATVIGDSLVAQWAFERMRGLGKE
                     TSECLFFLATAELSQGREEHAMATLERAVRLNSRNGRALLLLGKLRSDRGQLELARES
                     LRSASACPETSYEANRALAKVCRSLRLDAEARDAESKARGGRPRPAGGLTLFQDR"
     gene            complement(7412..8764)
                     /gene="rnc"
                     /locus_tag="VFM00_04590"
     CDS             complement(7412..8764)
                     /gene="rnc"
                     /locus_tag="VFM00_04590"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET7497556.1"
                     /translation="MNFIRKLLGLDGPKPPARGSARDRDFDLRGLERILHYNFKNTQL
                     ARQAMTHRSYLHATPGRSGESNERMEFLGDSVVGLVVNEFLYKKFTKLREGELTKMKS
                     LLVSRVILSRAANQLGLGHYILLSDAEKGSGGRNRASILADTLEGVIGAMYLDGGIEP
                     ARKLTERLLLREVNAILSDANLANYKSMLQEYVQGEFKTHPQYRISSEYGPDHQKMFT
                     VEVVVSGKTYGRGHGSNKKEAEQEAARDALFHFEKLANPVKEKAADGEVADKGAAAGD
                     RRRRRRGGRGRRNGRGREGEAVSAIGTPHVGPASGAAPAHGTSGGGHGRHTQGGERRG
                     RRSRDRDRDRESPQRETPREFEREPVHEESPRDREAPRDYESPGDHDAPRGYDAPREH
                     EPSHREHEPSHEDVSHERERSDDHRSDDHRSDDHRSGERSEKPEPGHGSPYAAARLRE
                     "
     gene            complement(8800..>9517)
                     /locus_tag="VFM00_04595"
     CDS             complement(8800..>9517)
                     /locus_tag="VFM00_04595"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET7497557.1"
                     /translation="IIQRGEADVMITGGSEAPITPVAFAGFCSMKAMSTRNDEPTRAS
                     RPFDSERDGFVMGEGAGILVLEEYEHAKRRGATALAEIVGYGATGDAHHMTAPAPEGE
                     GAARAMRAAVCDSGLSLESFGYMNAHGTSTPLNDKFETQAIRSVFGEHAKKLAISSTK
                     SMTGHLLGAAGGLETIICVLALQSQCLPPTINYEHPDPDCDLDYVPNTARPVELQSAL
                     SNSLGFGGHNVTLALSRTVA"
BASE COUNT         1351 a         3318 c         3404 g         1444 t
ORIGIN      
        1 ccgcgcccag gaagccgctc gaatccagcc gggccaggat ctcccggtgc agccccgcag
       61 cctcccgggt gttgcctgtc gcccggagcg cctcggccag catgctgagc gcctgctccc
      121 ggttcgactc atcgggcgcc gcctcggccg ccgcgggggg agccgcggtg tgcgcctcgc
      181 gcgcgggcgc cgacgcggcc accatctcgc cgccggggcg gcggatccgg atggtcgaaa
      241 accagcggcg cacctcgacc tcggccaggt cctcctcgcg catgagcttc accagctcgg
      301 tgatctgcct gcgaaggttc atcgcccgta tcctcgtcct cgcttgccgg cgcggagcag
      361 cgcgtcgagg gcccgaaggc ccagctcgta gctcatggcg cccagtccca tgatcacgcc
      421 gcgcacggcg gagccggtga ggctggtctg ccgctcccgc tcgcgcgcgt agagattggt
      481 gaggtgcact tcgacgaccg ggatcgtaac cgagcggagc gcgtcggcga gcgccaggct
      541 ggtgtgcgtg aacgccgcgg ggttcagcag gcagccgtcc gcctcgtcca tcgcgatcgt
      601 caggcggtcg atcagcgctc cctcgtggtt ggtctggaag ctggtgacgt tcaggttcag
      661 ctcctcgccc agcgcggcga gccgcttgtc gatggccgcg agcgcgttct tgccgtagag
      721 ctcgggttcg cggcgtccca gaagacccag gttcggtccg tggagcaccc agacgtcccg
      781 cgttcccgcc atcaagcccc cccgcccagc cggcggagcc agtcctcgaa cgccgagcga
      841 ttcccgcgca ccggctcgtc gggcggcgcc ggcgtgctcg gcgcgctcgg agcccgtccg
      901 gccgaagacc cgggcgacga gaacgcgggc cgcggcggag cgtcgggctc ggggagcgcg
      961 aggtcgggcg cgggcggcga gacgtcggac ggcgcgatgc cggacggcgg gaggtcggac
     1021 ggagcgatgc cggacgacgc gacgtcggac gcgggagcgc gggacgcggg aggcgcgggc
     1081 gtggccatgg ggcgctcggg catcgccggc gcgtccaggc tcagctcctg gaagctccgc
     1141 gcgcgatcgc gatcggatcc ggacgcggcc gactcctccc cgaaggtctc gatcgtccga
     1201 agtcccaggc cgagcgcgga gaggtgctcc tcttcgcgcg gcaggcgcac cgggtgccag
     1261 cccaggtctc cctggccgac gaccacgtcg gcgggacaga cgtcggcgta ctcgcccgag
     1321 gtgatctgct ggagcgcggt ctgggattcg agatcgccgg gatcgacgcg gcagacggcg
     1381 cggtagagct cgaccgagcg ggcgtgccgc ccctcgtggg ccaggatccc cgcgagcgcc
     1441 ttcagcgcgg agacgttctc gcgatcgagc gcgagcgccg cttcgaacga ggaacgcgcc
     1501 tcgttcaggc ggccatcggc cagatagcag cggccgagga cgatcgcatc gccgaccccg
     1561 tgtccgggcc ggttctgccg cgcctcgcag aggcggatcg cctcttcgac gcgcccctcc
     1621 tcgcgaaggc gctcggccag cggcagatag agcgcggtcg agggatcgcg ctctaccatt
     1681 tcttccaatc gtttcgtacg ggtttcggcg agttggtcgg gcatggtctc cccacgctca
     1741 ggatcgcatg acgaggcttt cgacgacgcg ccgaagggag gtaagcgacg acaactcaaa
     1801 tatttcggcg ctgccgatcg cggggcacaa taccactcgg agacgcgtcc gtccacgctt
     1861 cttgtccctc gcgagcgcat tccagaagcg ctcgccggga aaacgcggga gccgggtcgg
     1921 gagcccaagc ctggcgatca gccgctcggt ctcctgcgcg tcgaccggat cgagtcccgc
     1981 gtggagcacg ctcatccgga gcgcggccac catgcccacg gcgaccgcct ccccatgctg
     2041 gtagagacgg tagccgcccg ccgcttcgag cgagtggccg acggtgtggc cgaagttgag
     2101 ctcgcggcga agccccgtgt cgcgctcgtc ctgctcgacg taccacgcct tggtggccgc
     2161 gcattcgcgc aggatcggcg ccagggaggc ggcggccgcg tcgcgggccg cgcgcttgcc
     2221 cgcgagcgac tcggcgatgg cgtcgaggcg gcgcaggatc gccggccggc ggatcatccc
     2281 catcttgacc acctccgcca gccccgaacg gaaggcgcgg cccgggagcg tggcgagcgc
     2341 ttcggggtcg gcgaagacgg cgcgcggctg gtggaaggcg ccgaggaggt tcttccccgc
     2401 ggccaggttc acgccggtct tccccccgat cgccgcgtcg gcctgcgcca gggtggtcgt
     2461 gggaaaggcg atccacggaa gcccgcgcgt gtaggccgcg gcggcgaatc ccgccgtgtc
     2521 ggagacgacg ccgccaccca gcgccacgac caccgcctcg cgtgtcaggc ccgcgcgcgc
     2581 ccatccctcg gccagtacct gcacggtctc gaaccgcttc gatccctcgc ccggagcgat
     2641 cgtggtccgg acgacgcgga atccggcgcg gcggagcgag gcctcgaccc gggcaccgta
     2701 gaggcgcgcg accgtgcgat cggtcaccag cgccacgctc cgcccgcggg ccccggcgcg
     2761 tccaccccgc gcgcgccgcg cgcgcccgcg cggttcttcg agggccgcgc gcacgcacgc
     2821 gccggcgcgc gcgagcagcc cgcgctcgac gcgcaccacg tacgacgacg cggcgccgcg
     2881 cacgcggacg cggatcgcgc gggatttcga tcgggatgaa ggggagcgga ccgacatgga
     2941 cggtcgcagg ctagcacacg ccgcggggag acttcaccaa acgccgcgaa atctagcgat
     3001 tctggcccag cgaatcggac gcttccatcc cccggaggcg ctcttccagg gcccgctgtt
     3061 cctcgcgcag cttgagcagg tgctcccgaa gcgccttctt gtcggcggcg ttcagggcgg
     3121 gcggcgcctc ggagcgcgcc gctccctgcg ggcgggggcc ggtgatgttg gagcccacga
     3181 cgatgcggag cggaaggatc tcggcgtcgc gcaggatggt cacctcggtg tcgtacccgg
     3241 gacgcagcgt ggcgaccatg gagaccagct cctccgcgga gcgcacttcc tggccgtcca
     3301 tcgcgaggat gtggtccccg acgcggacgc ccgcgacggc ggccggactc ccggcgatca
     3361 cgtcgtcgac gcgcacgccg agcgtgtcct cgggagacac cgggccccgc ttcaaccgga
     3421 tgcccaggaa gccgcgctgc acggtgccgt gctcgcccag atcggcgacg atccgctcca
     3481 cctgatccac cggaacggcg aggagcaccc cgccggtcgg gggccttccc ggccgctcgg
     3541 gatcgccgac gcgcgagctc tcgccggcgt tgggaccctg gagcccgcgg cccacgacca
     3601 gcgccaccca ctcgccgtcc tcgttcacga cggcgccgcc ggccgaggcc gggtagccgg
     3661 gcgcgtcgat ctcgatcacg tcgcccgagt agggaaagtc cacccgctcg ccgcggccga
     3721 cgacctgtcc gatcgcggcc tgcgggcggg acacggtgat gttcgagatg accgccaccc
     3781 aggtgccgac cgcgagcgat tgcggagccg cgcggcggag cgtgtggagg accggctctt
     3841 ccagccggaa cacggcgagg ttcgactggc ggtccacgcc gcgcaggacg gcgcggcgct
     3901 cgacgccgcc gcccagcatc acgtgcacgt cgccgttggg gaaggcgagg ccggcggtgg
     3961 tgacgatgtc gcgctcgttg gtggcgacgc cggtgccgat caggcgcttg cgcttcacgc
     4021 ccgcgggcgc gctgtgctgc gcctcgggaa cggcgacgat ggtcaggatg gaagcctgga
     4081 gcgagtcgag cacgcccagg aaggggctcg attcggggcg gagcgggccg ggcgcggcgg
     4141 aggcggcggc gcccccgagg aacggcgcgg cgagaattgc gagtcccgcc gcggcggcgc
     4201 ggagcttcat cgcggcgcta ggcccggtgc ttcgatcaga acgtgatgta cacgtcatcg
     4261 gtggtgcgac ccgagacggg cacgatcgag tgaactccgt cttccccgga gcgattcagg
     4321 tagaactgat ccaggatgta ctcgtcctgg tacggggcgt tgatccgggc ggagtcggcc
     4381 gatcccgacg cggcggccgt tccttccagc gcggccggaa cggagcccgt gcgggacggc
     4441 gccacgcgcg cgggcgtggg agcggaagcg accacgtcgc cggcgggagc cgcgggcgtc
     4501 gtgacggcgc tcgagggagc cggaacgttg gaggtcgcgg tctgttcggt cttgggagtc
     4561 gtgcccgcga tcttgctgcc gccgttggga gccgcgtcct ggccggggtt cacgagcagg
     4621 aacgcgatcc agagcgcgca acccgcggcg ccggccagga acaccggacg gagccgcgcc
     4681 ggggcgagat gctccgaaat ccactcgatc aggccggggc gcatggcttc gcccgaacgg
     4741 atgcgatcca tgatctcttc ctcgaacgcg ggacccgtct cataggacgg gagcgtgctc
     4801 aggagatcca ccgtgacgcg cagctcctgc accgcggccg agcagcgcgc gcattcctcc
     4861 agatgactct cgagggaaag gcgctccctc tggctcagct cgtcttcgac gaacgacgag
     4921 aagagatgtt ccgcggaccg gcagttcata cgtcctccag tttcaagtag ggcttgagcc
     4981 gttccttgag catcagtcgc gcgcggttga tccgcgatcg cacggtcccg ccggggatgc
     5041 ggaggatctc gccgacttcc tcgtagctga gtccttccac gtcccggagc acgaccgcct
     5101 cgcgatagcg cggggggatt ttgcgaagcg cctcggcgac cttgttctgt atttcacgcg
     5161 cattcagatc cgcgtcgggc agcggcttcc agtcggggaa cgggagcccc tgcccgccgg
     5221 actcctcgtc catggcgtcc aggctgtacg tgccgcggtg ccttaccctg ctccggatct
     5281 cgttcttcgt gagattgacg gcgatcgtga agatccacgt cgagaacttg ccgctcttgc
     5341 ggtagcgctc cttgttgcgg aagacgcgga ggaagacctc ctgcgcgatc tcctcggccc
     5401 ggtcccggtc gttgatgaac cgacagacca gattcatgat tcgagagcgg taacgctgca
     5461 cgagggtgcg gaaggccgcc tcggagcctt gggcacaccg ctccatgacg tcttcatcgc
     5521 tgagctcggc gagagggtcg agctttttca atcgcaagga ctcgatgggc acgttcggct
     5581 ccggactagc cctaggtacc ccgccccggg agggcaggtt cctgcattcc cgggggttag
     5641 cgatcctgga agagggtgag gcctccggcc gggcgagggc gtccgccgcg ggccttggat
     5701 tccgcgtcgc gggcctcggc gtccaggcga aggctcctgc aaacctttgc caaagcgcgg
     5761 tttgcttcgt aggaggtctc gggacaggcg gaggcggagc ggagggactc ccgggccagc
     5821 tccagctgtc cccggtcgct ccggagcttc cccaggagca gcagggcgcg cccgtttcgg
     5881 gaattcaggc ggaccgcacg ctccagggta gccatggcgt gctcctcccg gccctgcgac
     5941 agctccgccg tggcaaggaa gaacaggcac tcggaggtct ccttccccag gccgcgcatg
     6001 cgttcgaacg cccactgggc gaccaacgag tccccgatga cggtcgcggc cgagcccagt
     6061 tcctcgaagg tcgaggggct cgagattccg cgggaggccg aatcgcggat cagggccacc
     6121 gcttcgtccg ggtaaccgag cagagagtag cacaggctcg cggtcaggac ctgacgcggg
     6181 gtgtcgagac gcgctgaccg cgcgcgctcg agcgtctcca ccccgcgcgc gcgatccccc
     6241 acgcgaatct cgagataggc cacgaccagc gactcctccc gcgagagcgt gcgtccgggc
     6301 accagcgtgc ggtagcactc cagcgctttc cgatcctcgc ccagggagac gtacgcgcgg
     6361 gcgcgaaccg gcatcgcgcg cgggtccccc ttcaggggac ggacggtgtc gagcgcctcg
     6421 gggtagcgcc cctcgcgaac cagggcctct cccgtctcgg cgcgcaggtc ggcggcgggc
     6481 atcgggcccg cgtcggccca cgcctcgagc tgccggaacg agtccttctt ccccatgcgg
     6541 accaggaagg caccatacgc ggcgatgtcc gtcggccgcg ccgtggactg gagcgccagg
     6601 gtgcggagga ggcgctcttc caggaccggg tcgtcggtgc gctgcgcgag cgtgacgccc
     6661 aggtgcagca cgcgcgggtc gtcggaccgc agctccaggg ccaactgaag ggcctgcgac
     6721 gcttcctcca gccggccatc ctcgagcgct aagccgagga tcccggtcgc gatccacgca
     6781 aagtcgggca ggagctcgtc ggtcctcttc agatccgcga gggcggcgtc atagcggcgg
     6841 gaaagctcgt gggccagcgc ccggagggcg tacgcctcgg gcggggcgcc caggatccgg
     6901 agcggggggg tcagcgcctg ggcggcgatg tcgctctccc ctccgcgcag gtgcatccgc
     6961 gccacgagca tggccgcctc gagattcaga ctgtcccggg ccagggcctt ctgcccccag
     7021 gagagggcct cgctgtcctg gccgatggtc tcgaggaggg tcgccgcgcg cgcgcaaccg
     7081 aaggcgctcc cggggtctgc cgtcgccagc gattccgccc actggatggc cgcctccccg
     7141 tccccggcag cctcggccag ctcggagcgg gtcaggaggt cgagcagaac cccatccggc
     7201 ggggccccgg aaagcgacgg gtagccggat tccatgcgcg aggcggcgtg tgcgaccggg
     7261 gggacgaagg cgggcgccgt gaagccgaga acgagaagga agaggctctg tcgaaggctg
     7321 gtggtcacga atcgtctccc gaaaaaggac gaggaaggcg gtcagccttc ctcccctctc
     7381 gagacgatgc gtggattata ccagccggtg cctactcgcg caagcgtgcc gccgcgtagg
     7441 gtgatccgtg accgggctcc ggcttctcgc tgcgctcgcc ggaacggtgg tcgtcggagc
     7501 ggtgatcgtc cgaacggtgg tcgtcggaac gctcccgctc gtgggaaacg tcctcatggg
     7561 acggctcatg ctcccgatgg gacggctcgt gctcccgcgg cgcgtcgtag ccccgcggcg
     7621 cgtcgtgatc gcccggcgat tcgtaatccc gcggcgcttc gcgatcgcgc ggggactcct
     7681 cgtgcaccgg ctcccgctcg aactcgcgcg gggtttcgcg ctgcggcgat tcccggtcgc
     7741 ggtcccgatc ccgcgagcgg cggcctcgac gctcgcctcc ctgcgtgtgg cgtccgtgac
     7801 ctccgccgct cgtgccgtgc gccggtgcgg cacccgaagc gggtcccaca tgcggcgtcc
     7861 cgatcgcgga gacggcttct ccctcgcgtc cgcgcccgtt ccggcggcca cgtccgccgc
     7921 ggcgccggcg gcgccgatcg cccgcggccg cgcccttgtc cgcgacctcg ccgtcggcgg
     7981 ccttctcctt gacgggattg gcgagcttct cgaagtggaa gagcgcgtcg cgcgccgctt
     8041 cctgctccgc ttccttctta ttgctcccgt gcccgcggcc gtaggtcttc ccgctgacga
     8101 cgacctcgac cgtgaacatc ttctgatggt cggggccgta ctcgctggag atccggtact
     8161 gcggatgggt cttgaactcg ccctgcacgt actcctgcag catgctctta tagttggcga
     8221 gattcgcgtc gctcaggatg gcgttcacct cgcgcaggag gagccgctcc gtgagcttcc
     8281 gcgccggctc gatccctccg tccagataca tggcgccgat caccccttcg agggtgtcgg
     8341 ccaggatcga ggcgcggttc cgcccgcccg agcccttctc ggcgtcggag agcaggatgt
     8401 agtgccccag cccgagctgg ttggcggcgc gcgagaggat gacgcggctc acgaggagcg
     8461 acttcatctt cgtcagctcc ccctcgcgca gcttggtgaa tttcttgtaa aggaactcgt
     8521 tcacgacgag cccgacgacg gaatcgccga ggaattccat ccgctcgttc gattcgccgc
     8581 tccgtccggg ggtggcgtgg agataggagc gatgggtcat cgcctgacgc gcgagctgcg
     8641 tgttcttgaa gttgtaatgc aggattcgct cgaggccccg cagatcgaaa tcgcgatcgc
     8701 gggcgctgcc tcgggcgggc ggcttggggc cgtccagtcc caacagcttt cggatgaagt
     8761 tcacagtgtt cggagccggg gtcgcccgcg ggctccgcct tatgccacgg tcctcgacag
     8821 cgccagggtg acgttgtgcc caccaaagcc cagagaattg ctcagggcgg actgcagctc
     8881 gaccgggcgt gcagtgttgg ggacgtagtc gaggtcacag tcgggatcgg ggtgctcgta
     8941 gttgatcgtc gggggaaggc actggctctg gagggcgagg acgcagatga tcgtctcgag
     9001 tcctcccgcc gcgcccagaa gatggcccgt catcgacttc gtcgagctga tcgccagctt
     9061 cttcgcgtgt tccccgaaca ccgacctgat ggcttgtgtt tcgaatttgt cgttgagtgg
     9121 cgtggaggtg ccgtgggcgt tcatgtagcc gaacgactcc agggacagtc ccgagtcgca
     9181 gacggccgcc cgcatggccc gcgccgcacc ctcaccctcg ggggcgggcg cggtcatgtg
     9241 atgggcatct cccgttgcgc cgtacccgac gatctccgcc agagcggtcg cgccgcgccg
     9301 cttcgcgtgc tcgtattcct cgagcaccag aatgccggcg ccttccccca tcacgaatcc
     9361 gtctcgttcg gaatcgaagg ggcgcgaggc tcgggtcggt tcgtcgttgc gcgtggacat
     9421 cgccttcatg gagcagaaac cggcgaacgc gacgggtgtg atcggcgctt cgctgccgcc
     9481 cgtgatcatc acgtccgctt cgccgcgctg gatgatc
//
LOCUS       DASSLZ010000123         4111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_41976, whole genome shotgun sequence.
ACCESSION   DASSLZ010000123 DASSLZ010000000
VERSION     DASSLZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41976"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="VFM00_04600"
     CDS             complement(<1..1542)
                     /locus_tag="VFM00_04600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HET7497558.1"
                     /translation="MTTVTAADLDLTAADVAWDLEPLVQGDGDAGVNRLLDEAERRAT
                     ALAAAYHGRIATLDAAAFAALMREIAEISDRLGRASNYAGLRFAENTEDPARGALLAL
                     VEERGTAISNELIFVTLEWAALEARRAEALLADPALEFCRHHLVVARRHHPHLLTEPE
                     ERILADKGVTGRSAWTRLFEELTSSITVTLDGATMGLEQALARLHSADREVRRAAAEA
                     TTAALQPGLRTRAYVLNTLLSDKAIDDRHRRYANWLQSRNLDNEASDESVEALIGAIE
                     SRYDIPRRWYTLKARMLGLDRLADFDRYASVAEHETQHGWKESRELVLEAYRSFSGEL
                     ATGVKRFFDEPWIDAPVRPGKQTGAFCSYTVPSAHPYVLLNWTARRRDVLTLAHELGH
                     GIHAYLARPQGVFHQSTPLTIAETASVFGETLTFGKLLESTTDPGARLALLAESLEGM
                     IATVFRQIAMNRFEEAAHTERRERGELSVERFGELWAETQGRMMGDSIVVTEGYRSWW
                     SYIPHF"
     gene            complement(1553..3853)
                     /locus_tag="VFM00_04605"
     CDS             complement(1553..3853)
                     /locus_tag="VFM00_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET7497559.1"
                     /translation="MKGSVAAIVLLLLAMEAASTPARATAATPGAATPGAAAPAPGAV
                     ADTLSFEFADPSGAPAGWRVWMPPMRWDPGAPHGGRAAARIDRDSTSADDFSAFSREI
                     PADFAGDTLELRGWLRTENVRGFAGLWLREDGRGGSVQFDNMEERHIAGTSPWTEYRV
                     ALPLESRARSIVFGGLLVGTGTAWMDEMRLLVDGKPIAEAPRVVRTPPGAETDHEFDA
                     GSRLTERELLDARVENLALLGKVWGFLKVHHPRVAAGALNWDYELFRILPAVAKARTR
                     DAAARAMVRWIDRVGMPPPCRPCATLSDSAVIAPSIGWIHSTSRLTRDLSTRLERIYR
                     DRSTGDDAYYASLTGLSNPDFSNENGYKALDAPDAGYRLLALFRFWNVIEYWFPDRDL
                     LREPWDGVLAEFIPKLLHAADRDAYRLAMIDLVVRIRDGHANVWNAIDVRPPRGGCRL
                     PVALRPVGDRYVVGAFADSVRGPASRFRIGDVIRALDGAPLDSAAAAWAPHYAASNEP
                     TRRREISRWATQGPCGACRVSVEREDGVHEILAQRDSSRTMNGRAGSTHDLPGPAFRR
                     LADDVAYLKLSAVRAADAASYVQQAAGTRCFVIDIRNYPSEFVVFALGQHLVATPTPF
                     VRFTRADLANPGAFFGMGPVVIEPAAPHYEGKIVILVDEATQSSAEYTSMAFRAVPGA
                     IVVGSTTAGADGNVSTLRLPGGLGAAMSGLGVFYPDHTPTQQVGIVPDLVVHPTVEGI
                     RAGHDEVLEAALKLALDREVRVPSAP"
     gene            complement(3850..>4111)
                     /locus_tag="VFM00_04610"
     CDS             complement(3850..>4111)
                     /locus_tag="VFM00_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7497560.1"
                     /translation="PEAAAILRSVRRAAAPGATLLVIEMIVPETPGPDMAKTIDVHMM
                     ALFGGRQRTRAEYESLLGAAGFALKRELETEAGVSILEAVAQ"
BASE COUNT          608 a         1448 c         1480 g          575 t
ORIGIN      
        1 gaagtgcggg atgtaggacc accaggagcg gtagccctcg gtgacgacga tggaatcgcc
       61 catcattcgt ccctgcgtct ccgcccacag ttcgccgaag cgctccacgc tcagctcccc
      121 cctctcgcgc cgctcggtgt gcgccgcctc ctcgaagcgg ttcatggcga tctggcggaa
      181 caccgtcgcg atcatcccct cgagactctc ggccaggagc gccagccgcg cgccggggtc
      241 ggtcgtgctc tcgagcagct ttccgaacgt cagcgtctcc ccgaacaccg aggcggtctc
      301 cgcgatcgtg agcggcgtac tctggtggaa gaccccctgg ggccgcgcca ggtaagcgtg
      361 gatcccgtgc cccagctcgt gcgccagcgt gagcacgtcg cgccgccggg ccgtccagtt
      421 cagcaggacg tagggatgcg ccgagggcac cgtgtaggag cagaacgccc cggtctgctt
      481 gccggggcgc acgggcgcgt cgatccaggg ctcgtcgaag aaccgcttca cgccggtcgc
      541 gagctcgccg gagaaggagc ggtacgcctc cagcaccagc tcgcgcgact ccttccagcc
      601 atgctgcgtc tcgtgctcgg cgaccgacgc gtagcggtcg aagtcggcca ggcgatccag
      661 cccgagcatc cgcgccttga gcgtgtacca ccgccgcgga atgtcgtagc ggctctcgat
      721 cgccccgatc agcgcctcga ccgactcgtc gctcgcttcg ttgtccaggt tccggctctg
      781 gagccagttc gcgtagcggc ggtgccggtc gtcgatcgcc ttgtcggaga ggagcgtgtt
      841 caggacgtac gcgcgggtcc ggagtcccgg ctggagcgcc gccgtcgtcg cctcggccgc
      901 ggcgcgccgc acctcgcggt ccgcggagtg gagccgcgcg agcgcctgct ccaaccccat
      961 cgtcgcgccg tcgagggtca cggtgatcga agaggtcagc tcttcgaaga gccgtgtcca
     1021 ggcgctccgc cccgtcaccc ccttgtcggc cagaatccgc tcctcgggct cggtgagaag
     1081 gtgcggatgg tggcgccgcg ccacgacgag atgatgccgg cagaattcga gcgccgggtc
     1141 ggccaggagc gcttcggcgc gacgcgcctc cagggccgcc cactccagcg tgacgaagat
     1201 cagctcgttc gagatcgccg tgccccgctc ctcgacgagc gcgagcaagg caccgcgcgc
     1261 gggatcttcg gtgttctccg cgaagcgcag tccggcgtag ttcgaagcgc gcccgaggcg
     1321 gtcggagatc tcggcgatct cgcgcatcag ggccgcgaac gccgccgcgt cgagggtcgc
     1381 gatgcggccg tggtacgcgg cggcgagagc cgtcgcgcgc cgctcggctt cgtccagcag
     1441 gcggttcacg ccggcgtccc cgtcgccctg gacgagcggc tccaggtccc aggcgacgtc
     1501 ggcggcggtg agatccagat cggcggcggt cacggtggtc atggggagtt cctcatggag
     1561 cggaggggac gcgtacctcc cgatcgaggg ccagcttcaa cgccgcctcg agcacctcgt
     1621 cgtgtcccgc gcggatgcct tcgaccgtgg gatggacgac gagatcggga acgatcccca
     1681 cctgctgcgt cggtgtgtgg tcggggtaga aaacgccgag gccgctcatg gcggccccca
     1741 gccctccggg cagccggagg gtcgagacgt tgccgtcggc gccggcggtg gtgctgccga
     1801 cgacgatcgc gcccgggacc gcccggaacg ccatcgacgt gtactcggcg gagctctgcg
     1861 tcgcctcgtc gacgagaatg acgatcttgc cttcataatg gggtgccgcg ggctcgatca
     1921 cgaccggccc catcccgaag aacgcgccgg gattcgcgag atccgcccgc gtgaagcgga
     1981 cgaacggggt cggagtggcc acgaggtgct gccccagcgc gaagaccacg aactcgctcg
     2041 gatagttccg aatgtcgatc acgaagcagc gcgttcccgc cgcctgctgc acgtacgacg
     2101 cggcgtccgc cgcgcgcacg gcggagagct tgagataggc gacgtcgtcc gccaggcggc
     2161 ggaacgccgg cccaggaagg tcgtgggtcg atcccgcgcg gccgttcatg gtgcgcgacg
     2221 agtcgcgctg ggccaggatt tcgtggacgc cgtcctcgcg ctccaccgag acgcgacacg
     2281 cgccgcaggg gccctgggtc gcccagcgtg agatctcgcg gcgccgcgtc ggctcgttgg
     2341 aggcggcgta gtgcggcgcc caggcggccg cggccgaatc gaggggcgcg ccgtcgagcg
     2401 cccggatgac gtcgccgatg cggaaccggc tcgcggggcc gcgcacggaa tcggcgaagg
     2461 cgccgaccac gtagcggtcg cccaccggcc gcagcgcgac cgggagacgg cagccgccgc
     2521 ggggcgggcg gacgtcgatc gcgttccata cgttggcatg gccgtcccgg atgcggacca
     2581 cgaggtcgat catcgcgagg cgatacgcgt cccgatccgc ggcgtggaga agtttcggga
     2641 tgaactcggc caggacgccg tcccacggct cgcggagcag atcgcgatcc gggaaccaat
     2701 actcgatcac gttccagaat cggaagagcg cgagcagccg gtagcccgcg tcgggagcgt
     2761 ccagagcctt gtagccgttc tcgttcgaga agtcgggatt cgaaaggccg gtcagggagg
     2821 cgtagtaggc gtcgtccccc gtggaccggt cgcggtagat ccgctcgagc cgcgtcgaga
     2881 gatcccgcgt gagacggctc gtgctgtgga tccagccgat ggagggcgcg atcacggcgg
     2941 agtcggaaag ggtcgcgcac ggccggcagg ggggcggcat ccccacgcga tcgatccagc
     3001 ggaccatggc gcgcgccgcg gcgtcgcgcg tcctggcctt cgcgaccgcc ggcaggatgc
     3061 ggaagagctc gtagtcccaa ttgagcgctc cggccgccac ccgcggatgg tggaccttga
     3121 ggaagcccca gacctttccc aggagcgcca ggttctccac ccgcgcgtcg agcagctccc
     3181 gctcggtcag gcgcgagccc gcgtcgaact cgtgatcggt ctcggcgccc ggcggcgtcc
     3241 gcacgacacg gggcgcctcg gcgatcggtt ttccgtcgac gaggagccgc atctcgtcca
     3301 tccacgccgt gcccgtcccg accaggaggc cgccgaacac gatgctccgc gcccgcgact
     3361 ccagcggcag cgccacgcgg tactcggtcc agggcgacgt gccggcgatg tgacgctcct
     3421 ccatgttgtc gaactgaacc gagccgccgc gcccgtcctc gcgcagccag agtcccgcga
     3481 acccccgcac gttctcggtg cgcagccacc cgcggagctc gagcgtgtcc cccgcgaagt
     3541 ccgcggggat ctcccgcgaa aaggcggaga aatcgtcggc gctcgtggaa tcgcgatcga
     3601 tccgcgccgc ggcgcgaccg ccgtgcggcg ccccgggatc ccaccgcatc ggaggcatcc
     3661 agacccgcca cccggcgggg gctcccgagg gatccgcgaa ctcgaacgac agggtgtcgg
     3721 cgacggcgcc tggggcgggg gcggcagcgc ctggggtggc ggcgcctggg gtggccgcgg
     3781 tggcgcgagc gggcgttgaa gccgcttcca tcgcgaggag gagaaggacg atggccgcca
     3841 cggatccctt cactgcgcca ccgcctccag gatggagacg ccggcctcgg tctcgagctc
     3901 ccgcttcagg gcgaagccgg cggccccgag caggctctcg tactcggcgc gggttcgctg
     3961 acgcccgccg aacagagcca tcatatgcac gtcgatcgtc ttcgccatat cgggcccagg
     4021 cgtctcggga acgatcatct cgatcacgag gagcgtcgcc ccgggagccg cggcgcgccg
     4081 gaccgagcgg agaatggcgg cggcttccgg a
//
LOCUS       DASSLZ010000124        11759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_928317, whole genome shotgun sequence.
ACCESSION   DASSLZ010000124 DASSLZ010000000
VERSION     DASSLZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11759
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928317"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1049)
                     /locus_tag="VFM00_04615"
     CDS             complement(75..1049)
                     /locus_tag="VFM00_04615"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET7497561.1"
                     /translation="MNAQTAVAESRTAARPGPGRVREILSWYASDNPGSKANLARMMN
                     HGLLAGTGRMVILPVDQGFEHGPARSFAPNPAGYDPRYHVELAIESGCNAYAAPLGFL
                     EAGAADYAGEIPLILKLNNHDVLHDEKDPLSSVTGSVGEALRLGCAAIGFTIYPGSSQ
                     AGHMYEQIRKLAEEAKRVGLAVVVWSYPRGSDLSKDGETAIDVVSYAAQIAAQLGAHV
                     IKVKLPTAHLEQTEAKKVYEKYSIPIGTLAERVRHVVQSSFDGRRIVIFSGGAKKDSD
                     EDVLQEARAIRDGGGFGSIIGRNSFQRKRTEAIQLLHRIMAIYSGDAA"
     gene            complement(1102..2091)
                     /gene="glk"
                     /locus_tag="VFM00_04620"
     CDS             complement(1102..2091)
                     /gene="glk"
                     /locus_tag="VFM00_04620"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HET7497562.1"
                     /translation="MNDGLVLAADVGGTKTRLALVPALGRGRIGDPSAETSVASADFP
                     SLEALLEDFRASHRARITAVSVGYAGPVTEGRAAGTHVPWTADESSLERRFGAPAYVI
                     NDLVASGYGVPALGAGDLETLLRGSPGEPGNAAIVSAGTGLGESTLLRHAGDWIPVAS
                     EGGHADYAPRTDLEIEVFRALRDEFGRVSVERVLSGLGLGNIARVLHRREGAESALAK
                     HLDEAGPEELPAAISANGLAGACPSCVRSLELFVSAYGAEAGNAALRGMAVSGVYLGG
                     GIAPKILAALRWPLFRDAFLAKEPHRELLERVPVFVVLTDRSALLGAARYATL"
     gene            complement(2114..3238)
                     /locus_tag="VFM00_04625"
     CDS             complement(2114..3238)
                     /locus_tag="VFM00_04625"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HET7497563.1"
                     /translation="MNETPAAGRAPADTTAPFLLLGDQGERPFDAVALERELESMWKS
                     PPGANAFYRAALANLIVPLDTEPFERLADVIADVARRHPARLFRIGPARHAPGEPKRL
                     TARATALCHLREGGGAFVCSEQVLLEYTEETAPLVPSAVRSLLIGDLPVVLLLLTPGP
                     RPPWAEALAGLADIVVADSCVEEEPAAMAAVWERTGRKGAPMHDLAWARLEPWRASLA
                     SLFDTPEAAPALRSLHDVTVVHGGAAPPSSAWLLAGWLASRLGWKVASRDGARFRFRA
                     PEGGTPEIAFVRDETDPEARIREVRARAAGAHPLDARLTHEARADDARLELLAPRALV
                     TPIPFVRRELAAAVVGEIQRRIPNAAFRDAARAARALLEA"
     gene            complement(3235..4782)
                     /gene="zwf"
                     /locus_tag="VFM00_04630"
     CDS             complement(3235..4782)
                     /gene="zwf"
                     /locus_tag="VFM00_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET7497564.1"
                     /translation="MSPDAGARVSAAPNPLREGSVTLRAAEPCTFVVFGASGDLTHRK
                     LLPALLELTRDRLLHPKTAVVGFARQAKTDQEFRDETAALAKEHDIAEAVWHAFAPSL
                     FYCTGTFENGDDYKALKAFLEKVEKERGIPGNRIYYLAAPPPAYPAIFENLGASGLAR
                     PAEGAWTRVIVEKPFGRDLRSARDLNAKIHAHFDERQIYRIDHYLGKETVQNILVFRL
                     ANGIFEPLWNSRYVNHVQITVAETVGVEARAGYYEQAGVLRDMLQNHMLQLLALTAME
                     PPSRFDADFVRDEKAKVLRAMEPLTPEQVAKRTVRGQYAAGSVAGEAVPGYRQEPGVD
                     PNSTTETYLAVKLEVDNWRWAGVPFYLRSGKRLGKRATEVAISFRRPPYSLFRSIGCA
                     PIEANVLRLRIQPDEGVSLSFGTKAPGQALQIQPVRMDFNYLTAFGADPPDAYERLLL
                     DCNLGDSTLFAREDEVELAWERVDAIVRAWAERGDPPLLPYAAGTWGPREGDALPERD
                     GHRWYRV"
     gene            complement(4779..5696)
                     /gene="gnd"
                     /locus_tag="VFM00_04635"
     CDS             complement(4779..5696)
                     /gene="gnd"
                     /locus_tag="VFM00_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET7497565.1"
                     /translation="MPAGAAMEIGFVGLGRMGKNMVTRLLRGGHRVVAWDPNASAVAE
                     VAGAGAVAASGLGDVIAQLRDRPRAVWVMVPSGQPTEQVIHDLGQALDRGDIVVDGGN
                     SHYKESVRRGALLRERGVTFVDSGTSGGIWGLENGYCLMVGGDEAACKRLEPAFLTLA
                     PPGGYARVGESGAGHFVKMIHNGIEYGLMEAYAEGFEILSASDYRLDLPRIASIWRYG
                     SVVRSWLLDLAADALQEDPKLDHVKDYVDDSGEGRWTVVESVERAVPAPVLTLALQAR
                     FRSRQSSSFAGKMLAALRHKFGGHAVKTT"
     gene            complement(5696..6841)
                     /gene="tal"
                     /locus_tag="VFM00_04640"
     CDS             complement(5696..6841)
                     /gene="tal"
                     /locus_tag="VFM00_04640"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET7497566.1"
                     /translation="MKPTPATALHELGQSVWLDNINDGWIQSGELARWIAEGSVYGVT
                     SNPTIFKNAISGDKYSYPDEIRRLASAGQSPESIYEALAVRDIARTADLLAESYRRGT
                     GRDGFVSLEVEPDKADDTQATIEQARHYRQIVNRPNLFVKVPGTEAGVPAIEALIAEG
                     ISINVTLLFDRSQVAAAAEAYLRGIQTRLDKGQSIEGLHSVASVFVSRLDTSVDKELD
                     AKAAAAGAAADRERYLSLRGSAAVANALDVWEDYRARFFGPGFEDLKRRGARPQWMLW
                     ASTGTKNPAYSDVKYVEELAGPDSINTMPKETLDAWLEHGAVQGNRLDPRVETARATR
                     RVLADAGIDLAAHCRKLQTEGVALFEKSFAEMMDTIRERSAALQARR"
     gene            complement(6868..7410)
                     /locus_tag="VFM00_04645"
     CDS             complement(6868..7410)
                     /locus_tag="VFM00_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7497567.1"
                     /translation="MPARKKASRKSAARKKPAARAASRKKSVARKPAAPKRAARSTTA
                     PRRAAAPPAVPSAIGMSVHHFDFTTHDIDAVKRFYVELLGFETHEHDPQFNYLWVQTG
                     GTSSLGFMPPMPGMGEPTPPKEPTLYFIVEDVDRAYATLSARGVMFEGPPADMPWGHR
                     VVRTIDPEGRPVMLATRKQT"
     gene            complement(7590..9341)
                     /locus_tag="VFM00_04650"
     CDS             complement(7590..9341)
                     /locus_tag="VFM00_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET7497568.1"
                     /translation="MAATTQQTELLGSLEGAYRETLESLRNGDAVARLFARDASLWKD
                     DPAHAAVIRNRLGWLDEPGRLRGRIAELQVFSADVRAASFTRVLLLGMGGSSLAPEVL
                     KRCMTPGPGAPILDVLDATDPDAIRSAEAGARLDRTFFLVSSKSGTTLETLSQYRFFR
                     SRLEGAGIADAGSRFAAITDPGSPLEALARAEGFRKIFRNPSDIGGRYSALSYFGMVP
                     AALLNLDLSALAARAEAAREDSLSPDPVRNGALRLGALLGAAAREGRDKLTILTAPFL
                     RPVGFWIEQLIAESTGKEAKGIVPVEGEPLGAAHHYGADRVFVSIELEGEPVPDLERL
                     SGELSRSGAAWVRIVLPGRDSIAGEFFRWEVATSFAGAVLRIDPFDEPNVQESKDNTK
                     RILDQFTTAGVMPAGQPVARDLGIEVYAEADFWSRIVEGSPAHPSLEMVLHRFLTRAR
                     AGEYVALLAFMERTAASEASFALIRRAIRNALRVPVLQGYGPRYLHSIGQLFKGGPRT
                     GLFLVLTASGETDLPIPGSPYTFGQVETAQALGDLASLASRGKPALRLHMTQGAQAGL
                     EALGAAVERAIAAAAQV"
     gene            complement(9343..9795)
                     /gene="rpiB"
                     /locus_tag="VFM00_04655"
     CDS             complement(9343..9795)
                     /gene="rpiB"
                     /locus_tag="VFM00_04655"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET7497569.1"
                     /translation="MTIAIGADHAGFEQKERVKRILEARGHQVLDMGTDSTESTDYPA
                     YAFKVAEAVREGTAERGVLACDSGNGIAIAANKVEGIRAAIAINRWQAEMARRHNDAN
                     VLVLASAVTPKDQEEAILDAWLNAPFDGGRHARRVAQIGEYERTHERR"
     gene            9933..10427
                     /locus_tag="VFM00_04660"
     CDS             9933..10427
                     /locus_tag="VFM00_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET7497570.1"
                     /translation="MAKSESGRPTAPLRAEHAMIMSKVQALAERIETLHEQTTLDQLS
                     TAREVVDFVRRQIAPHAHAEEYTLYPAADWAAGEGSKLTEPVRFEHVLVSRRCDALEK
                     AIGAGAAPGKLMHLCYGILGLVAAHFVVTEEVILPYLDKAFDGPRFEKEVLTPLRETR
                     KTKR"
     gene            complement(10498..>11759)
                     /locus_tag="VFM00_04665"
     CDS             complement(10498..>11759)
                     /locus_tag="VFM00_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET7497571.1"
                     /translation="NMAVLDSLFAKRYELARLLGYKTWADYVTEDKMIRTPKRAAEFL
                     DKVGALVADQSKREYGVYLKRKREDDPKATAVENWEVSYYGNLIRKRDYDFDAQSARP
                     YFAFDRVKQGVLDVTSRIFGVTYKRIENAVVWDPSVEAYEVYEGDRLLGRFFFDLHPR
                     PGKFNHAAQFTIRQGCAGIQLPEAALVCNLPGGKPGDPGLMEHADVVTFFHEFGHLLH
                     TIFGGGQRWEPIAGISTEWDFVEAPSQMLEEWAWYPSSLQTFAKHYQTGEPIPAAMVD
                     KMRRADAFGRGIDRGFQTYLSEVSLHVYDRPPAQVNSDSVVAQAERAIVPFPPVPNTH
                     MQCSFGHLDGYSAIYYTYLWSQVIAKDLFTRFDREHLLEPSVPTRYRKTVLAPGGSKP
                     ADTLVRDFLKRDFNTKAFDAYVREAMD"
BASE COUNT         1706 a         4062 c         4217 g         1774 t
ORIGIN      
        1 cccgagcacg cgacccactt cccgcaggat tcctttccct cgcgccatcg cgtagcgcca
       61 ggctgccctt agcgctacgc ggcatccccc gagtagatcg ccatgatccg gtgcaagagt
      121 tggatagcct cggtcctctt ccgctggaac gaattccgcc cgatgatcga gccgaacccg
      181 cccccgtccc ggatggcgcg tgcctcctgc agcacgtcct cgtcgctgtc cttcttcgct
      241 ccgcccgaga agatcacgat gcgccggccg tcgaaggacg actggaccac gtggcgcacc
      301 cgctcggcca aggttccgat cggaatcgag tacttctcgt agaccttctt cgcctccgtc
      361 tgctcgagat gagcggtggg gagcttgacc ttgatcacgt gggcgccgag ctgggccgcg
      421 atctgggcgg cgtaggacac cacgtcgatg gcggtctctc cgtccttgga gaggtccgag
      481 ccgcgtggat aggaccagac gaccaccgcc agccccacgc gcttggcctc ctcggcaagc
      541 ttgcggatct gctcgtacat gtgtccggcc tgcgacgagc cggggtagat cgtgaatccg
      601 atggcggcgc agcccaggcg cagggcctcg ccgacgctcc cggtcaccga ggagagggga
      661 tccttctcgt cgtgcaggac gtcgtggttg ttcagcttca ggatgagcgg aatctcgccc
      721 gcgtaatccg cggccccggc ttccaggaat ccgagcggcg ccgcgtaggc gttgcacccc
      781 gactcgatcg ccaactccac gtggtagcgc ggatcgtagc cggcggggtt gggggcgaag
      841 gagcgggcgg ggccatgctc gaagccctgg tccaccggca ggatcaccat ccggcccgtt
      901 ccggcgagaa gcccgtgatt catcatgcgg gccaggttgg ccttgctccc cggattgtcc
      961 gaggcgtacc aggaaaggat ctcgcgcacg cggccggggc ccggccgggc ggcggtacgg
     1021 ctttcggcga cagcggtttg tgcgttcatg cgggccatta taggccgccg ggaggggccc
     1081 cggcaaggcg ggtgggcgat gctacagcgt cgcgtaccgc gccgcgccca gcagggccga
     1141 gcgatcggtc aacaccacga acacaggcac ccgctcgagc aactcccgat gcggctcctt
     1201 ggccaggaag gcgtcccgga agagcggcca ccgcagcgcg gcgagaatct tgggcgcgat
     1261 ccccccgccc aggtagaccc ccgagaccgc catgccccgg agcgccgcgt ttcccgcctc
     1321 cgccccgtac gcggagacga acagctcgag ggagcggacg caggaggggc aggcaccggc
     1381 cagcccgttc gcggagatgg ccgcgggaag ctcttccggt cccgcttcgt ccaggtgctt
     1441 ggcgagagcg ctctcggcgc cctcccgccg gtggaggaca cgagcgatat tgccgaggcc
     1501 caggcccgat aggacgcgct ccaccgagac ccggccgaac tcgtcgcgca gggcgcgaaa
     1561 cacctcgatc tcgaggtcgg tgcggggggc gtagtcggcg tgccccccct ccgaggcgac
     1621 cggtatccaa tccccggcat ggcggagcag cgtgctctcg ccaagccccg tgcccgccga
     1681 gacgatcgcc gcgttgcccg gctccccggg gctcccccgg aggagggtct ccagatcccc
     1741 agctcccagg gcgggaaccc cgtaccccga ggccacgagg tcattgatca cgtaagccgg
     1801 ggcgccgaac cggcgctcga gcgagctctc gtccgccgtc cagggaacgt gggtgccggc
     1861 cgcccgtccc tccgtgacgg ggcccgcgta tcccaccgag acggcggtga tgcgcgcgcg
     1921 atggctcgcg cggaagtcct cgagcagcgc ttcgagggaa gggaagtccg cgctcgcgac
     1981 ggacgtttcc gccgagggat cgccgatcct gccgcgcccc agggcgggaa cgagcgccag
     2041 gcgcgtcttg gtcccgccga cgtctgcggc gaggacgagg ccgtcgttca tggcgtgatc
     2101 cgcggtgggc ggctcacgct tcgagcaggg cgcgcgcggc gcgggccgcg tcgcggaagg
     2161 cggcgttggg gatgcgacgc tggatctcgc cgacgacggc ggccgcgagc tcgcggcgaa
     2221 cgaaggggat cggcgtgacc agggcgcgcg gcgccaggag ctcgaggcgc gcgtcgtccg
     2281 cgcgcgcttc gtgcgtgagg cgggcatcca gcggatgagc gcccgcggcc cgggcgcgca
     2341 cctcgcggat ccgggcctcg gggtcggtct cgtcgcgcac gaaggcgatc tccggagtcc
     2401 cgccttcggg ggcgcggaaa cggaatcggg cgccgtcgcg cgaggcgacc ttccatccca
     2461 ggcggctcgc cagccagccg gcgaggagcc aggcggagga cgggggagcc gcgcccccgt
     2521 gcacgaccgt gacgtcgtgc agggagcgga gcgcgggcgc ggcctcgggc gtgtcgaaga
     2581 ggctggccag ggacgcgcgc cagggctcga ggcgcgccca ggcaaggtcg tgcatcggcg
     2641 cccccttccg tcccgtgcgc tcccataccg cggccatcgc ggcgggctcc tcctcgacgc
     2701 agctgtcggc gacgacgatg tccgcgagcc ccgcgagcgc ctccgcccag ggcggccgcg
     2761 gccccggcgt gagcaggagc agcacgaccg ggaggtcgcc gatcaggagc gagcggaccg
     2821 cgctcggcac gaggggtgcg gtctcctcgg tgtactccag cagcacctgc tccgagcaga
     2881 cgaacgcgcc gcccccctcg cgcaggtggc agagcgcggt ggcgcgcgcg gtgaggcgct
     2941 tcggctcgcc cggagcatgg cgcgcgggac cgatgcggaa gaggcgcgcc gggtggcgcc
     3001 gcgccacgtc ggcgatcacg tcggccagcc gctcgaaagg ctccgtgtcc agcggcacga
     3061 tcaggttcgc cagggcggcg cgatagaagg cgttcgcgcc cggaggcgac ttccacatcg
     3121 attcgagctc gcgctccagg gccacggcgt cgaagggacg ctcgccctgg tcgccgagaa
     3181 gcaggaaggg agccgtggtg tcggcgggag cgcggcccgc ggcgggcgtc tcgttcacac
     3241 gcgataccag cggtggccgt cccgctcggg gagcgcgtcg ccttcgcgcg gtccccaggt
     3301 tccggcggcg tagggaagga gcggcggatc gccccgctcc gcccaggccc gcacgatcgc
     3361 gtcgacccgc tcccacgcca gctccacctc gtcctcgcgc gcgaagaggg tcgagtcgcc
     3421 caggttgcag tcgagcagca ggcgctcgta ggcgtcgggc ggatcggccc cgaacgcggt
     3481 gaggtagttg aaatccatcc gcaccggttg gatctgcagc gcctgccccg gtgccttggt
     3541 tccgaacgag agcgacacgc cctcgtccgg ctggatacgc agccggagca cgttggcttc
     3601 gatcggcgcg cagccgatcg accggaagag ggaatagggt gggcggcgga aggagatggc
     3661 cacttccgtg gcgcgcttgc cgagtcgctt ccccgagcgc aggtagaagg gcacccccgc
     3721 ccagcgccag ttgtccacct ccagcttcac cgcgagatag gtctcggtcg tggagttggg
     3781 atccacgccc ggctcctgcc ggtacccggg caccgcctcg cccgccaccg agcccgcggc
     3841 gtactgcccg cgcacggtgc gcttcgccac ctgctcaggg gtgagcggct ccatggcgcg
     3901 aagcaccttt gccttctcgt cgcgcacgaa gtcggcgtcg aaccgcgacg gaggctccat
     3961 cgcggtcagg gcgagcagct ggagcatgtg gttctggagc atgtcgcgca gcacgccggc
     4021 ctgctcgtaa tagcccgcgc gcgcctcgac gccgaccgtc tcggcgacgg tgatctgcac
     4081 gtgattcacg tagcggctgt tccagagcgg ctcgaagatc ccgttcgcga gccggaagac
     4141 caggatgttc tggaccgtct ccttgccgag atagtggtcg atgcgataga tctgccgctc
     4201 gtcgaagtgg gcgtggatct tcgcgttcag atcgcgcgcc gagcggagat cgcgcccgaa
     4261 gggcttctcc acgatgacgc gcgtccacgc gccctcggcg ggacgcgcca gtcccgaggc
     4321 gcccagattc tcgaaaatgg cgggataggc gggaggcggc gcggccaggt agtagatccg
     4381 gtttcccggg atgccgcgct ccttctccac cttctcgagg aaggccttca gggccttgta
     4441 gtcgtccccg ttctcgaacg tcccggtgca gtagaagagg gagggcgcga aggcgtgcca
     4501 gaccgcctcg gcgatgtcgt gttccttggc gagcgcggcc gtctcgtcgc ggaattcctg
     4561 gtcggtcttc gcctggcgcg cgaagcccac gaccgcggtc ttgggatgca gcaggcggtc
     4621 gcgggtcagc tcgagcaggg cggggaggag cttccggtgc gtgaggtcgc cgctcgcgcc
     4681 gaagacgacg aacgtgcagg gctcggccgc gcggagcgtg acgctccctt cgcgaagcgg
     4741 gttcggcgcg gcggagacgc gcgcgcccgc gtcggggctc acgtcgtctt gacggcgtgg
     4801 ccgccgaact tgtggcgcag cgcggcgagc atcttcccgg cgaacgagct ggactggcgc
     4861 gagcggaagc gcgcctgcag cgccagcgtg aggaccggcg cgggcaccgc ccgctccacc
     4921 gactcgacca cggtccagcg cccctcgccg gaatcatcca catagtcctt cacgtggtcc
     4981 agcttcgggt cttcctggag cgcgtccgcg gcgaggtcga ggagccagga gcggacgacg
     5041 cttccgtacc gccagatcga ggcgatgcgg ggtaggtcga ggcggtagtc ggacgcggag
     5101 aggatctcga agccttccgc ataggcttcc atcaggccgt actcgatccc gttgtggatc
     5161 atcttcacga agtggccagc gccgctctcg ccgacgcgcg cgtatccgcc cgggggagcg
     5221 agcgtgagga aggctggctc gagccgcttg caggcggcct cgtcgcctcc gaccatcagg
     5281 cagtagccgt tctccagacc ccagatgccg cccgaggtgc cggagtccac gaacgtcacg
     5341 ccgcgctcgc ggaggagtgc gccgcgccgc accgattcct tgtagtggga attgccgccg
     5401 tccacgacga tgtcgccgcg gtcgagcgct tggccgaggt cgtggatcac ctgctcggtg
     5461 ggctgccccg aggggaccat gacccagacc gcgcggggac ggtcgcgaag ctgcgcgatc
     5521 acgtcgccca gccccgacgc cgcgacggcg cccgcgccgg ccacctcggc cacggccgag
     5581 gcgttgggat cccacgccac cacccggtgc ccgcctcgga gcagccgggt caccatgttc
     5641 ttgcccatcc ggccgaggcc gacgaacccg atctccatcg ccgctcctgc cggcatcagc
     5701 gccgcgcctg gagcgcggcg gagcgttcgc ggatcgtgtc catcatctcg gcgaaggatt
     5761 tctcgaagag cgcgacgccc tcggtctgga gcttccggca gtgcgccgcc agatcgatcc
     5821 ccgcgtcggc cagcacccgg cgcgtggcgc gcgccgtctc cacgcgcggg tcgaggcgat
     5881 tgccctgcac ggcgccgtgc tccagccagg cgtcgagagt ctccttgggc atcgtgttga
     5941 tcgagtcggg cccggccagc tcctcgacgt acttcacgtc ggagtaggcg ggattcttcg
     6001 tgcccgtgga ggcccagagc atccactgcg gacgcgcgcc gcggcgcttg agatcctcga
     6061 agccggggcc gaagaagcgg gcccggtagt cctcccagac gtccagcgcg ttggcgacgg
     6121 cggccgagcc ccgcagggag aggtaccgtt cccggtcggc ggccgcgccg gcggccgcgg
     6181 ctttcgcgtc cagctccttg tcgaccgagg tgtcgaggcg gcttacgaag acgctcgcga
     6241 ccgagtgcag accctcgatc gactgcccct tgtccaggcg cgtctggatg ccgcggaggt
     6301 acgcttccgc ggccgcggcg acctggctac ggtcgaagag gagcgtgacg ttgatgctga
     6361 tcccttcggc gatcagggct tcgatcgcgg gcacccccgc ctcggtgccc ggcaccttca
     6421 cgaagaggtt cgggcggttc acgatctgcc ggtagtggcg cgcctgctcg atcgtcgcct
     6481 gggtgtcgtc cgccttgtcg ggctcgacct cgaggctcac gaacccgtcg cgcccggtcc
     6541 cgcgccggta gctctcggcg agcagatcgg cggtgcgcgc gatgtcgcgc accgccagcg
     6601 cctcgtagat cgactcgggg gactgtccgg ccgaggcgag acgccggatc tcgtcggggt
     6661 aggagtactt gtcgccgctg atggcgttct tgaaaatggt ggggttggag gtgacgccgt
     6721 acaccgaccc ctcggcgatc caacgggcga gctcgcccga ctggatccag ccgtcgttga
     6781 tgttgtcgag ccagacgctc tgtcccagct cgtgcaatgc ggtcgcgggg gtcggcttca
     6841 tgatgatggg gtcctttccc gagagatcta ggtctgcttc cgggtggcca gcatgaccgg
     6901 ccggccctcc ggatcgatgg ttcgcacgac gcgatggccc cacggcatgt ccgcgggcgg
     6961 cccttcgaac atcacgcctc gcgcgctcag cgtggcgtag gcacggtcca cgtcctccac
     7021 gatgaagtag agggtgggct ccttgggcgg ggtgggttcg cccatccccg gcatgggagg
     7081 catgaagccc aggctggagg tgcctcccgt ctgcacccag aggtagttga actgcggatc
     7141 gtgctcgtgg gtctcgaagc cgagcagctc cacgtagaag cgcttcacgg cgtcgatgtc
     7201 gtgggtcgtg aaatcgaagt ggtggacgct catcccgatg gcgctcggca ccgccggcgg
     7261 cgcggcggcg cgccgcgggg ccgtcgtgga acgcgcggcg cgtttgggtg ctgcgggctt
     7321 ccgggcgacg ctcttcttcc gcgaggccgc ccgcgcggcc ggcttcttcc gggccgcgga
     7381 cttgcgggaa gccttcttgc gagcaggcat ggaaccctcc ctgttcgagt cctaccggcg
     7441 tctgccggtc tgtgccccgg caggagcggt cccgcggcga gccgcgcctt cgaaccgccc
     7501 gtgccgatga tgccgtatcg cgtcgaaagg gcgcaaggcc ggcggacgtc cggcgcaagg
     7561 cactgctggg gcgatacttc gagggccttc tagacctgcg cggccgcggc gatggcgcgc
     7621 tccacggcgg ccccgagcgc ttcgagaccg gcctgcgcgc cctgggtcat gtgaaggcga
     7681 agcgcgggct ttcctcgcga ggcgagcgaa gcgagatcgc cgagcgcctg cgccgtctcc
     7741 acctgcccga acgtgtaggg gcttcccggg atcggcaggt cggtctcgcc cgaggccgtg
     7801 aggaccagga agaggccggt gcgcggcccg cccttgaaga gctgtccgat cgaatgcagg
     7861 tagcgggggc cgtagccctg gaggacggga acgcgaagcg cgtttcggat cgcgcgccgg
     7921 atcagcgcga aggaagcctc gctcgcggcg gtccgctcca tgaaggcgag gagcgcgacg
     7981 tactctcccg cgcgggcgcg ggtcaggaaa cggtggagca ccatctccag gcttggatgc
     8041 gcgggcgagc cctcgacgat gcgcgaccag aagtcggcct cggcgtagac ctcgatcccc
     8101 aggtcgcgcg cgaccggctg gcccgcgggc atcacgccgg ccgtcgtgaa ctggtcgagg
     8161 atccgcttcg tattgtcctt gctctcctgc acgttcggct cgtcgaaggg atcgatgcgg
     8221 aggaccgcgc ctgcgaacga ggtcgcgact tcccagcgga agaattcgcc cgcgatcgag
     8281 tcgcggccgg gcaggacgat gcggacccag gcggccccgg agcgcgacag ctctccggac
     8341 aggcgctcga gatcgggcac cggctctccc tccagctcga tcgagacgaa gacgcggtcg
     8401 gcgccgtagt ggtgcgccgc gcccagcggc tctccctcca ccggcacgat ccccttcgcc
     8461 tccttgccgg tgctctccgc gatcagctgc tcgatccaga aaccgacggg gcgcaggaag
     8521 ggggcggtca ggatcgtcag cttgtcgcgc ccctcgcgcg ccgcggcccc gagcagggcg
     8581 cccagccgga gcgcgccgtt gcggacgggg tcgggcgaga gcgagtcctc gcgggccgct
     8641 tccgcccgcg cggcgagcgc gctcaggtcc aggttcagga gcgcggccgg gaccattccg
     8701 aaataggaga gggcggaata gcgccctccg atgtcggacg gattgcggaa gatcttccgg
     8761 aaaccttcgg cgcgcgcgag cgcttcgagc ggcgagccgg gatcggtgat cgccgcgaag
     8821 cgggagcccg cgtcggcgat gcccgcccct tccaggcggg aacggaagaa gcggtactgc
     8881 gagagagttt ccagcgtcgt gcccgacttg ctcgagacga ggaagaaggt gcggtcgagg
     8941 cgcgcgcccg cctcggctga gcggattgcg tcggggtcgg tcgcgtcgag cacgtccagg
     9001 atcggggcgc cgggccccgg ggtcatgcag cgcttcagca cttcgggcgc gaggctcgac
     9061 ccgcccatgc cgaggaggag cacgcgggtg aagctggcgg cgcgcacgtc cgcggagaag
     9121 acctggagct cggcgatccg tccccggagg cgtcccggct cgtccagcca gccgagccgg
     9181 ttgcgaatga ccgcggcgtg ggccgggtcg tccttccaga gggaggcgtc gcgcgcgaag
     9241 agccgcgcga cggcgtcgcc gtttcgaagg gattccagcg tttcccggta ggcgccctcg
     9301 agggagccga gcagctcggt ctgctgcgtc gtggcggcca tgtcagcggc gctcgtgcgt
     9361 gcgctcgtac tcgccgatct gcgcgacgcg gcgcgcatgg cggccgccgt cgaacggcgc
     9421 gttcagccac gcgtccagga tcgcctcctc ctggtccttc ggcgtcaccg ccgaggccag
     9481 gacgagcacg ttggcgtcgt tgtggcgccg cgccatctcc gcctgccagc ggttgatggc
     9541 gatggcggcg cgaatgcctt cgaccttgtt ggccgcgatc gcgatcccgt tgccggaatc
     9601 gcacgcgagg acgccgcgct cggcggtgcc ctcgcgcacg gcctcggcga ccttgaaggc
     9661 gtacgccggg taatcggtcg actccgtgga atccgtgccc atgtcgagca cctggtggcc
     9721 gcgcgcttcc aggatccgct tcacgcgctc tttctgttcg aatccggcgt gatcggcgcc
     9781 gatcgcgatc gtcatgggcc cgctcctcgc tccgcgcgcg cgacgcgcgc acgcaggtct
     9841 ttcgagacta ggtccggtct cgaagacgcg caacagggga ttgcagcggg ggcattgaca
     9901 cgagggcgcg aaagccggag actgcgggac ccatggccaa atccgaatcc ggcaggccca
     9961 ccgcgccgct gcgcgcggag cacgcgatga tcatgtccaa agtgcaggca ctggccgagc
    10021 ggatcgaaac gctccacgag cagaccacgc tggaccagct ctcgaccgcg cgcgaagtgg
    10081 tggatttcgt ccgccgtcag atcgccccgc acgcccacgc cgaggagtac acgctctatc
    10141 cggccgcgga ctgggcggcc ggggagggct ccaagctcac cgagccggtc cgcttcgagc
    10201 acgtcctcgt ctcgcggcgt tgcgacgcgc tggagaaggc gatcggcgcg ggagccgcgc
    10261 cggggaagct gatgcatctc tgctacggga tcctcggcct ggtcgcggcg cacttcgtcg
    10321 tgaccgagga ggtgatcctt ccgtacctcg acaaggcctt cgacggaccc cgcttcgaga
    10381 aggaagtgct gaccccgctc cgggagaccc ggaagacgaa gcgctgaccg gggcggcgcg
    10441 cccgcgcccc tagtccatca ctcctgccgg tcccgagtcc atcgctccgg cgcgcaccta
    10501 gtccatcgcc tcccgcacgt acgcgtcgaa cgccttcgtg ttgaagtccc gtttcaggaa
    10561 atcgcgcacc agcgtgtccg cgggtttcga gccgccgggc gcgagcacgg tcttgcggta
    10621 gcgcgtcggc actgagggct ccaggagatg ttcgcggtcg aaacgggtga agaggtcctt
    10681 cgcgatcacc tgggaccaca ggtaggtgta gtagatggcg gaatagccgt cgagatgccc
    10741 gaacgaacac tgcatgtgcg tattgggaac gggcgggaac ggaacgatcg cgcgctcggc
    10801 ctgcgccacc accgagtcgc tgttcacctg cgcgggcggc cggtcgtaca cgtggagcga
    10861 gacttccgag agataggtct ggaagccgcg gtcgatgccc cggccgaagg catcggcccg
    10921 ccgcatcttg tcgaccatgg cggccgggat cggctcgccg gtctggtagt gcttggcgaa
    10981 ggtctgcagc gaggacggat accacgccca ctcctccagc atctgcgagg gcgcttccac
    11041 gaaatcccac tccgtggaga tgcccgcgat cggctcccag cgttggcctc cgccgaagat
    11101 cgtgtggagc aggtgcccga actcgtggaa gaaggtgacc acgtccgcgt gctccatcag
    11161 ccccgggtcg cccggcttgc ccccgggcag attgcagacc agggcggcct cgggcagctg
    11221 gatccccgcg cagccctggc ggatcgtgaa ctgcgccgcg tggttgaatt tgccgggacg
    11281 cgggtgcagg tcgaagaaga accttccgag gagccggtcc ccctcgtaca cctcgtaggc
    11341 ttccacgctc gggtcccaca cgaccgcgtt ctcgatccgc ttgtaggtga ccccgaagat
    11401 ccgcgaggtg acgtccagga ccccctgctt cacccggtcg aacgcgaagt aggggcgcgc
    11461 gctctgcgcg tcgaagtcgt aatcgcgctt ccggatcagg ttcccgtagt agctgacctc
    11521 ccagttctcc accgccgtcg ccttggggtc gtcctcgcgc ttgcgcttca ggtagacccc
    11581 gtattcgcgc ttcgactgat ccgcgaccag ggcgcccacc ttgtcgagga attcggcggc
    11641 gcgcttcggc gtgcggatca tcttgtcctc ggtgacgtag tcggcccacg tcttgtagcc
    11701 gaggagccgc gccagctcgt agcgcttggc gaagaggctg tccaggaccg ccatgttct
//
LOCUS       DASSLZ010000125         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_42505, whole genome shotgun sequence.
ACCESSION   DASSLZ010000125 DASSLZ010000000
VERSION     DASSLZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42505"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1315
                     /locus_tag="VFM00_04670"
     CDS             74..1315
                     /locus_tag="VFM00_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HET7497572.1"
                     /translation="MNILYLAHRIPYPPDKGDKMVSYHQVRHLGARHRLHLVCFADDR
                     RDLVHADVLREFCATVDVVYRSPRRARLEGLRGLLDGRAISVAAFDSAALRRAAAERL
                     RGADIVLAFSSVMAQYVPAGDWRPRVMDFVDADSEKWRLYASRSRWPASWLYAREADR
                     LGRFEEAVARSWDQSLFVCEREARVLRERVPGVPVGVIPSGVDLDYYAPPSRASSGNG
                     AGPRPERVPRLVFTGMMDYFPNVDGVRWFVSEVFPRVRAALPEARFTIVGRNPVHAVR
                     ALASQPGIEVTGAVPDVRPYLAAADVACVPLRVARGLQNKMLEAMAMGLPVVATTPAY
                     EGLDAPESAGIEVADDPAVFAERVVGLLRDDARRLEAGRRARAHVEARHKWADHGAAL
                     EALLEGLVASRPKPGRREAVS"
     gene            1386..>2643
                     /gene="asnB"
                     /locus_tag="VFM00_04675"
     CDS             1386..>2643
                     /gene="asnB"
                     /locus_tag="VFM00_04675"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET7497573.1"
                     /translation="MCGIAGIVELRGAVPDRGTLERMASLLAHRGPDEQGIAVIGQAG
                     LAHRRLSIIDLSGGHQPMADATGTLTLLFNGEIYNFMDVRSELESRGHRFATRSDTEV
                     LLQAYMEWGTDCVLRLNGMYAFVIWDAPRRRLFAARDRLGKKPFYYALTPDRFVFGSE
                     LKAVLAAPGVPRDVDPAAVDEFLSRYYVGGARTILRGIAKLPPAHWLSLEEGRLTVQR
                     YWRPAFRPDDPPRREEEYLEELEGLFRDAVRRRMISDVPLGAFLSGGVDSSAVVAMMA
                     AEGGPAVKTFTVGFEEAGYSEIEDARAVARHLGTDHHEETVRPDAIAILPDLVWHYDE
                     PFGDSSMVPTWYVARMARRHVTVALSGDGGDELFAGYTRYQRALEDRRLDWIPGPLKR
                     RVLAPLAEALPAGAPARNRLYAAAHHS"
BASE COUNT          332 a          943 c          974 g          394 t
ORIGIN      
        1 ccatccaggc ctggaggatg ctgcccctgc cggtcgccga gcggatcggc cccgcgatcg
       61 tccgctactt tccgtgaaca tcctgtacct ggcgcaccgc atcccctatc cgccggacaa
      121 gggggacaag atggtttcgt accaccaggt gcggcacctg ggggcgcgcc atcgcctgca
      181 cctggtctgc ttcgcggacg accgccgcga cctggtccac gccgacgtgc tgcgcgagtt
      241 ctgcgccacc gtggacgtgg tctaccgctc gccgcgccgc gcgcggctcg agggcctgcg
      301 gggcctcctg gacgggcggg cgatctccgt cgcggcgttc gacagcgcgg ccctccggcg
      361 cgcggcggcg gagcggctgc gcggcgcgga catcgtgctc gcgttctcct cggtcatggc
      421 gcagtacgtg ccggcggggg actggcggcc gcgcgtcatg gacttcgtgg acgcggattc
      481 ggagaagtgg aggctctacg cgtcgcgaag ccgctggccg gcctcctggc tctacgcgcg
      541 ggaggccgac cggctgggac ggttcgagga ggcggtggca cgttcctggg accagtcgct
      601 cttcgtctgc gagcgcgaag cccgcgtgct gcgggagcgc gtccccggcg tccccgtggg
      661 cgtgatcccg agtggcgtgg acttggacta ctacgctccg ccgtcgcggg cgtcgtcggg
      721 gaatggcgcc gggccccgtc ccgaacgggt gccgcgcctg gtgttcaccg ggatgatgga
      781 ctacttcccg aacgtggacg gggtgcgctg gttcgtctcc gaggtcttcc cccgggtgcg
      841 cgcggcgctt cccgaggcgc gcttcacgat cgtgggacgg aacccggtac acgctgtgcg
      901 cgcgctggcg tcgcagccgg gaatcgaggt gaccggtgcg gtaccggacg tccgccccta
      961 tctcgcggcc gccgacgtcg cctgcgtgcc gctgcgcgtc gcgcgcgggc tgcagaacaa
     1021 gatgctcgag gcgatggcca tgggccttcc ggtcgtggcg accacgccgg cctacgaggg
     1081 gctggacgcg cccgaaagcg cggggatcga ggtggcggac gatcccgccg tcttcgccga
     1141 gcgggtcgtg gggctcctcc gggacgacgc ccggcggctg gaagcgggcc gccgcgcccg
     1201 cgcccacgtc gaggcgcgcc acaagtgggc ggaccacggc gccgcgctcg aggcgctcct
     1261 cgaggggctc gtcgcgtcga ggcccaagcc gggacggcgg gaagcggtat catgaagggc
     1321 gcgatcggaa cgcggcgcct cctcgcccgg cgccggaccg gcgcatccac ccggagccac
     1381 gcctgatgtg cggcatcgcc ggaatcgtcg agcttcgcgg agccgttccc gaccggggga
     1441 cgctcgagcg catggcgtcg ctgctcgccc accgcggtcc cgacgagcag gggatcgccg
     1501 tgatcgggca ggcggggctt gcccatcgcc gcctctcgat catcgacctc tccggtgggc
     1561 accagccgat ggccgacgcc accggcaccc tgaccctcct cttcaacggg gagatctaca
     1621 acttcatgga cgtccggagc gagctggagt cccggggaca ccgcttcgcg acgcgctccg
     1681 acacggaggt gctgctccag gcctacatgg aatggggcac cgactgcgtg ctccggctga
     1741 acgggatgta cgcgttcgtg atctgggacg cgccccgccg ccggctgttc gcggcgcggg
     1801 accggctggg gaagaagccg ttctactacg cgctcacgcc ggaccggttc gtcttcggct
     1861 ccgagctcaa ggccgtgctc gccgcgccgg gcgtgccgcg cgacgtggat cccgccgcgg
     1921 tggacgagtt cctctcccgc tactacgtgg gtggggcgcg gaccatcttg cgcgggattg
     1981 cgaagctccc gcccgcgcac tggctcagcc tcgaggaggg gcgtctcacg gtccagcgtt
     2041 actggcgtcc cgccttccgc cccgacgatc cgccgcgccg cgaggaggag tacctcgagg
     2101 agctggaggg tcttttccgc gacgcggtcc gccgccggat gatcagcgac gtgccgctcg
     2161 gcgccttcct ctcgggcggc gtggattcca gcgccgtcgt ggcgatgatg gccgccgaag
     2221 gaggtccggc cgtgaagacc ttcacggtcg gattcgagga ggccggctac tcggagatcg
     2281 aggacgcgcg cgccgtggcc cgccacctgg gcaccgacca ccacgaggag acggtgcgcc
     2341 ccgacgcgat cgcgatcctt cccgatctcg tgtggcacta cgacgagccg ttcggcgatt
     2401 cctcgatggt gccgacctgg tacgtggccc gcatggcgcg ccgccacgtg acggtggcgc
     2461 tctcgggcga tggcggcgac gagctcttcg ccgggtacac gcgctaccag cgggcgctgg
     2521 aagatcgaag gctcgactgg atccccgggc cgctgaagcg gcgcgtgctc gcccccctgg
     2581 ccgaggcgct cccggccgga gcgccggcac gaaaccggct ctacgccgcc gcgcatcatt
     2641 ccg
//
LOCUS       DASSLZ010000126         6736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_799989, whole genome shotgun sequence.
ACCESSION   DASSLZ010000126 DASSLZ010000000
VERSION     DASSLZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799989"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1238
                     /locus_tag="VFM00_04680"
     CDS             66..1238
                     /locus_tag="VFM00_04680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HET7497574.1"
                     /translation="MGATLILGAGFGGLACARALRARTPRDHRILLIDRSPLFVVGAA
                     KTWVMLGERRADEVMKTRAALVPDGVELLREEVKRVDAAARRVETDRGSHAGDHLVIA
                     LGAELDMAGVPGLDAAAHTFYTLDGAARIRQALDSFHGGRLLLLVPRTPFACPPAPYE
                     AAFLLHALLERRGIRGATQLDVITVEKAPMMTAGPFMGRMIVGQLESRAIGFHGQKKA
                     ASVDAKSRVVRFEDGSEAAYDLLLAVPPHRVPRILVEAGLAEAGGWVRVDPKTLEVSG
                     NSHVYAIGDSTVLALPGRFDPGAPPLALPKAGVFAAAEGEAVAARIASRLTKGSEPAP
                     FDGHGFCYVEMGGRRAMRAEGDFFAEPNPVMTAREPNEEQYRDKVAWIEGWLRPQN"
     gene            1316..2494
                     /gene="pgsB"
                     /locus_tag="VFM00_04685"
     CDS             1316..2494
                     /gene="pgsB"
                     /locus_tag="VFM00_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HET7497575.1"
                     /translation="MVLLPAALATGLTLYGLVESRRHRRNLDRIPIRVHVNGTRGKSS
                     VTRLIASGLRAGGIRTFAKTTGTMARMIRPDGSEADVYRVGPPNVIEQKRIVRRALEE
                     RAEALVIECMAVHPELQPLCERRLVRSTVGVITNVRADHLDVMGPTVDDAAAALGQTL
                     PFQGTAFTAERDRFAALAAIARERGSRLHGVDGGDVSARELDRFSYDEHAENVALALA
                     VCAHLGVPRDVALQGMWAATPDPGVLRRYHVEAGMKQITFVNAFAANDPDSTKIVWTR
                     LGLDQAEPGVRRIVLANCRGDRVQRSGQIAELLALGLPSDDVVLTGQGTALVAQRAIA
                     LGLDRARVHDLGGMSAERVVLRVFALVERRATVVGIGNIVGLGEDLLLEFQSRAVGDD
                     "
     gene            2487..2936
                     /gene="pgsC"
                     /locus_tag="VFM00_04690"
     CDS             2487..2936
                     /gene="pgsC"
                     /locus_tag="VFM00_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HET7497576.1"
                     /translation="MIEQAIGLGLAISLLFSETLGLAAGGMVVPGYVALLVHEPLRLV
                     GTILVSLVTLGTLKLIAQYTLIYGRRRIVAAVLLGFIFGAISRDLLVFNVGGTHVELQ
                     TIGFVIPGLIANWMDRQGIVQTLCVMVTTAVLVRLLLMLLNGGALIR"
     gene            2933..4057
                     /gene="pgsW"
                     /locus_tag="VFM00_04695"
     CDS             2933..4057
                     /gene="pgsW"
                     /locus_tag="VFM00_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HET7497577.1"
                     /translation="MSQVRASTPTLVALAAGSLLLYAVAERSLTPVHAEAYVKKLRAV
                     RIMQRAEHTLAAWKMSRGIAIDERNDPSTTGVIGPQFTLTTTDRGSQAAKVVAAHPNF
                     AAAVTQMMLQAGVREGDLVAVGLTGSLPGLNLAVLSACRAIGAEPVVIASVGASMFGA
                     TDPDFTWLDMESALDARDVWHYRSLAASLGGGGDVGRGLSPAGRRLLLDSIDRNRAQL
                     IDAPNLMDAVRRRVALYDSVAAARGRPIKLYVNVGGGLASLGGAQNARLVPAGLTFRL
                     AARNYPNRGVLNVMAERRVPVIHLLEVERLAARLGIVDRDTGATPDAGKGLLFVQYRY
                     NLWVVALAAASILAANVFVLRLDLRRRLMGQPHPERADAP"
     gene            4054..4983
                     /locus_tag="VFM00_04700"
     CDS             4054..4983
                     /locus_tag="VFM00_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497578.1"
                     /translation="MKRNAHRLLAALSLALIAAPSAAASRWRALERIAGQEPVTVTVE
                     GAGRGYYRLDRVTPLEIPVTGPARLQVITRAEIAPGREGATYRVWGDLDGTRLREERT
                     ESAPARKASIAGAGRVVCKSRTFSWEIPAGEHRLRIFGDLPPVGARGGSGGAVLARLR
                     MSVPESGPLPMVSLTPVDAFRSVTLAEGETLIPYYTITSAKPVRVRVVGPTDLELTAR
                     LDFDSTMRGEQSYRVQVWEAGRRLRQFDWRTTKASTASYTDLRDRVASKMSRATFPVG
                     EGTHELLVHLVEPKQGSAEIRIRIPQPTAGESE"
     gene            4980..6152
                     /locus_tag="VFM00_04705"
     CDS             4980..6152
                     /locus_tag="VFM00_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497579.1"
                     /translation="MKPRPLSIRARSGASQRLVPLFVAGPLAAALLLGAVPSRAGSSR
                     LLLGLGVSTWYDDNTLQYSADQIALLDSGTRPERFSVKSADDPALSPSVSLALNDRLG
                     KGRTRTIRVRGQGEFHQNSGTADFRSGSLLWQEPVSRGSRVSLSGYYTPHFYLRQLRD
                     DDITAVGVNPYRRAEFSLAIGALRWRQKAGRRAALEASYQFEHRGYNPDFVERTSDTH
                     EGKLAWGWSRLGGRGSASVHGGYRASDARGADGDETPGSLPDDPDVGYHGILAGVDGL
                     IDLEPHRARGFTLGLGYELATRDYTSKVASDTYHAGRKDVDHTLDASLRWSARGRFAV
                     RGFYRFETNDARLGGAAPATSDVGSFTENQVGIAVEWSKVLWRSSAGGVDSDDDTP"
     gene            complement(6156..>6736)
                     /locus_tag="VFM00_04710"
     CDS             complement(6156..>6736)
                     /locus_tag="VFM00_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7497580.1"
                     /translation="VFDLDGVLVDSFQCWWRLLNDTLEAHGQRPLTEEEFSVCWGQDV
                     EADRRRFFPDWTVEGLIEHYTREFPRYASWIRPMEGAQETLDRLERAGKAIAVASNSP
                     MAMVERLLELASLRQHVRWAAGVDLVPNGKPEPDLIEHVLREAGMARDDACFVGDSDF
                     DARAARAADVYFIGYRRPGDARIESLAELIAP"
BASE COUNT          973 a         2364 c         2362 g         1037 t
ORIGIN      
        1 gggcatgagc agcctccaac tatcctcgct tcggcccccc aggggaaggg agtaagatgg
       61 cgcccatggg cgcgaccttg attctcggcg cgggcttcgg cggcctcgcc tgcgcgcgcg
      121 cgcttcgtgc ccgaaccccc cgcgaccatc gcattctgct gatcgaccgg tcccctctgt
      181 tcgtggtggg ggcggcgaag acgtgggtga tgctgggcga gcgccgcgcc gacgaggtga
      241 tgaagacgcg cgcggcgctc gtgcccgacg gggtcgagct cctgcgcgaa gaggtgaagc
      301 gcgtggacgc cgcggcccgc cgcgtcgaga ccgatcgcgg cagccacgcg ggcgatcatc
      361 tcgtgatcgc gctcggcgcc gagctggaca tggccggagt gccgggcctg gacgccgccg
      421 cgcacacgtt ctacacactg gacggagcgg cgcgcatccg ccaggcgctc gactccttcc
      481 acgggggacg cctcctcctc ctcgtgccgc gcaccccctt cgcctgccct cccgcccctt
      541 acgaggccgc gttcctgctg cacgctctgc tggagcgtcg ggggatccga ggcgcgacgc
      601 agctcgatgt catcacggtg gagaaggcgc cgatgatgac cgccgggccg ttcatgggac
      661 gcatgatcgt cggccagctg gagtcgcgcg ccatcggatt ccacggccag aagaaggcgg
      721 cgtcggtcga cgccaagtcg cgggtggtgc ggttcgagga cggaagcgag gccgcgtacg
      781 acctgcttct cgccgtgccg ccgcatcggg tcccgcggat cctggtcgaa gcgggactcg
      841 ccgaggccgg aggatgggtg cgggtggatc cgaagacgct cgaggtgtcg gggaattccc
      901 acgtgtacgc gatcggcgac tcgaccgtgc tggcgctccc cgggcgcttc gatcccggcg
      961 ccccgccgct cgcgctcccg aaagcggggg tcttcgcggc cgccgagggg gaggccgtgg
     1021 cggcgcggat cgcgtcccgg ctcacgaagg gctcggagcc cgcgcccttc gacggccacg
     1081 gcttctgcta tgtcgagatg ggcgggcggc gcgccatgcg cgcggagggg gacttcttcg
     1141 cggagccgaa tccggtgatg acggcgcggg agccgaacga agaacagtac cgggacaagg
     1201 tggcctggat cgagggctgg ctccggccgc agaactgact ttctccgatc aagaaccccg
     1261 ccgattcggc cgataatcag gcaaacccca acacacatca ggagctgcgc accgcatggt
     1321 cctgctaccc gcggcgctgg cgaccggcct gaccttgtac ggtctcgtcg agtccaggcg
     1381 ccatcgcaga aacctggacc gcattccgat ccgggtccac gtgaacggca cccggggaaa
     1441 gtcctccgtg acgcgtctga tcgcctccgg gctgcgggcg ggaggaatcc ggaccttcgc
     1501 caagaccacc ggcacgatgg cccggatgat ccgcccggac gggagcgagg ccgacgtcta
     1561 ccgcgtgggt cctcccaacg tgatcgagca gaagcggatc gtccgccgcg ccctggagga
     1621 gcgggccgag gcgctggtga tcgaatgcat ggcggttcac ccggagttgc agcctctctg
     1681 cgagcgccgg ctggtgcgga gcaccgtcgg cgtcatcacg aacgtgcggg ccgaccacct
     1741 cgacgtgatg ggccccacgg tggacgacgc cgccgcggcg ctgggccaga cgctcccgtt
     1801 tcaaggcacc gcgttcaccg ccgagcgcga tcgattcgcg gccctcgcgg cgatcgcgcg
     1861 ggagcgcggc tcgcggctcc acggggtcga cggcggggac gtatctgccc gcgagctgga
     1921 ccgattctcc tacgacgagc acgccgagaa cgtggccctg gcgctggccg tctgcgcgca
     1981 cttgggcgtg ccgcgcgacg tggcccttca gggaatgtgg gcggcgaccc ccgaccccgg
     2041 cgtgctccgt cgctaccacg ttgaggccgg catgaagcag atcaccttcg tgaacgcctt
     2101 cgcggcgaac gaccccgatt cgacgaagat cgtctggacg cgactgggtc tggaccaggc
     2161 ggagccgggt gtgcgccgca tcgtcctcgc gaactgccgc ggcgatcggg tccagcggtc
     2221 gggccagatc gccgagctgc tcgcccttgg cctcccctcc gacgatgtgg tcctcaccgg
     2281 gcagggaacc gcgttggtcg ctcagcgggc gatcgcactc ggcctcgacc gcgcccgcgt
     2341 ccacgacctg ggcggcatgt ccgccgagcg ggtggtgctt cgggtgttcg ccctggtgga
     2401 gcgccgtgcc accgtggtcg ggatcgggaa tatcgtcggc ctgggagagg atctgctcct
     2461 ggagttccag agccgggcgg tcggcgatga ttgaacaggc gatcggcctc gggctcgcga
     2521 tcagcctgct gttctccgag acgctggggc tcgcggccgg cggaatggtc gtgcccggct
     2581 acgtcgcgct gctcgtccac gagccgctcc ggctggtcgg caccatcctc gtcagcctgg
     2641 tcaccctggg cacgctcaag ctcatcgccc aatacacgct gatctacggg cgccggcgca
     2701 tcgtggccgc cgtgctcctt ggattcatct tcggagcgat ctcgcgcgac ctgctcgtat
     2761 tcaatgtcgg cggcacgcac gtcgagctcc agaccatcgg gttcgtgatt cccggattga
     2821 tcgcgaactg gatggatcgc caggggatcg tccagaccct ctgcgtcatg gtgacgacgg
     2881 ccgttctggt acggctgctg ctgatgctgc tgaacggcgg ggcgctcatc cgatgagcca
     2941 ggtccgcgcc tccacgccga ccctcgtcgc gctcgcggcc gggtcgctcc tcctctacgc
     3001 ggtcgccgag cgttccctga ccccggtgca cgccgaggcg tacgtgaaga agctgcgcgc
     3061 cgtccggatc atgcagcggg ccgagcacac gctcgcggcc tggaagatgt cacgcgggat
     3121 cgcgatcgac gagcggaacg acccttccac gacgggcgtc atcgggcctc agttcacgct
     3181 gaccaccacg gatcgcgggt cccaggcggc gaaggtcgtc gcggcgcatc ccaacttcgc
     3241 cgccgcggtg acgcagatga tgctgcaggc cggggttcgg gagggggatc tcgtggccgt
     3301 gggcctcacg ggctccctac cgggactcaa cctggccgtt ctttcggcct gtcgcgccat
     3361 cggcgccgag ccggtcgtga tcgcgtcggt cggtgcctcg atgttcggcg ccaccgaccc
     3421 cgacttcacg tggctcgaca tggagtcggc gctcgatgcg cgtgacgtgt ggcactaccg
     3481 ctcgctcgcc gcgtcgctgg gcggcggtgg cgacgtcggg cgcgggctgt ctccggccgg
     3541 aaggcgcctg ctcctcgatt cgatcgatcg gaaccgtgcc cagctcatcg acgctccgaa
     3601 tctcatggac gcggtgcgcc ggcgcgtggc gctctacgac tccgtcgcgg ccgctcgcgg
     3661 gcgtccgatc aagctctacg tgaacgtggg gggcggtctc gccagcctgg ggggcgcgca
     3721 gaacgcgcgc ctggtcccgg ccgggctcac cttccggctc gccgcgcgca actatcccaa
     3781 tcgcggagtg ctgaacgtga tggccgagcg ccgggtcccg gtcatccacc tgctcgaggt
     3841 ggaacgactc gcggcccggc tcggcatcgt cgacagggat accggggcca cccccgacgc
     3901 gggcaagggg ctcctcttcg tccagtaccg gtacaacctc tgggtcgtcg ccttggccgc
     3961 ggcctccatc ctggccgcca acgtcttcgt cttgcgcctc gacttgaggc gccggctgat
     4021 gggtcagccg catccggaaa gggccgacgc cccatgaaac ggaacgcaca tcgccttctg
     4081 gcagccctca gtctcgctct gatcgccgcg ccgtccgccg ccgcgtcgcg ctggcgtgcg
     4141 ctggagcgca tcgccgggca agaaccggtg accgtgacgg tggagggagc ggggcgcggc
     4201 tactatcgcc tggaccgcgt cacgccgctc gagattccgg tgaccgggcc cgcacggctt
     4261 caggtgatca cgcgcgccga gatcgcgccg gggcgcgagg gcgccacgta ccgcgtgtgg
     4321 ggcgacctgg acggaacgcg cctgcgcgaa gaacgcacgg agagcgctcc cgcccggaaa
     4381 gcgtcgatcg cgggagccgg acgcgtggtg tgcaagagcc gcacgttctc ctgggagatt
     4441 ccggcggggg agcaccgcct gcgaattttc ggagacctgc ccccggtcgg cgctcgagga
     4501 ggcagtggcg gcgccgttct cgcgcgtctt cgcatgagcg tgccggagtc aggcccgctt
     4561 ccgatggtct ccctcacgcc cgtcgacgcc ttccgcagcg tgaccctggc ggagggagag
     4621 acgctgatcc cgtactacac gatcacctcc gccaagcccg ttcgcgtccg ggtcgtggga
     4681 ccgaccgacc tggagctgac cgccaggctc gacttcgact cgaccatgcg cggcgagcag
     4741 tcctaccgcg tgcaggtgtg ggaggccggc cgccggctcc ggcagttcga ctggcgcacg
     4801 accaaagcct cgaccgcatc ctacaccgac ctccgcgatc gggtcgcgtc caagatgagc
     4861 cgcgccacgt tcccggtggg ggagggcacg cacgagttgc tcgtccattt ggtcgagccg
     4921 aaacaagggt ccgccgaaat ccgcatccgc attcctcaac ctaccgcggg agagagcgaa
     4981 tgaagccacg tcccctcagc atccgcgctc ggtccggcgc ctcgcagcgc ctcgttcccc
     5041 tgttcgttgc cggtccgttg gccgccgcgc tgctcctcgg ggccgttccg tcgcgggcgg
     5101 gctcctcccg cctgcttctc gggcttggcg tctcgacctg gtacgacgac aacaccctgc
     5161 aatactcggc cgatcagatc gcgctcctcg attcgggcac tcgccccgag cggttctcgg
     5221 tgaaatcggc cgacgatccg gccctgagcc cctcggtctc gctggcactc aacgaccggc
     5281 tgggaaaggg ccgcacccgc acgatccgcg tccgcggaca gggtgaattc caccagaaca
     5341 gcggcaccgc cgatttccgc tccgggagcc tgctgtggca ggagccggtc tcgcgcggct
     5401 cgcgggtatc gctgtccggg tactacacgc cccacttcta cctgcgccag cttcgggacg
     5461 acgacatcac cgcggtcggc gtgaatcctt accggcgcgc cgagttctca ctggcgatcg
     5521 gcgcgctgcg atggaggcag aaagcgggac gccgtgcggc cctggaggcg agctaccagt
     5581 tcgagcatcg cggttacaac cccgacttcg tcgagcggac ctcggacacg cacgagggga
     5641 agctcgcgtg gggatggagc cggctgggcg gacgcgggag cgcatcggtg cacggcggct
     5701 accgcgcgag cgacgcccgg ggcgccgacg gcgacgaaac gcccggatcg ctccccgatg
     5761 atccggacgt ggggtaccac ggcatcctgg cgggcgtgga cgggctcatc gacctggagc
     5821 cgcaccgcgc gcgtgggttc acgctggggc tgggctatga gctggcgacc cgggactaca
     5881 cctcgaaggt ggccagcgac acctaccacg cagggcggaa ggacgtggat cacaccctcg
     5941 acgccagcct tcgctggagc gcgcgcggcc ggttcgcggt ccgtgggttc taccgcttcg
     6001 agacgaacga cgcgcgcctg ggaggggcgg ctcctgccac ctccgacgtg gggagcttca
     6061 ccgagaacca ggtcggaatc gcggtcgagt ggtcgaaggt gttgtggcgg tcctcggcgg
     6121 gaggcgtcga ttccgacgac gatacgccct gacgctcagg gtgcgatgag ctccgcgaga
     6181 gactcgatcc gcgcgtcgcc cgggcgtctg taaccgatga aatagacgtc ggcggcgcgg
     6241 gccgcccggg cgtcgaagtc ggaatcgccg acgaagcagg cgtcgtcgcg cgccatgccc
     6301 gcttcgcgca gcacgtgctc gatcaggtcg ggctcgggct tgccgtttgg aaccaggtcc
     6361 acacccgcgg cccagcgcac gtgctgccgc agcgaggcca gctccagcag ccgctcgacc
     6421 atggccatgg gcgagttgct cgccaccgcg atcgcctttc ccgcgcgctc caggcgatcc
     6481 agcgtctcct gggccccttc catcgggcgg atccacgacg cgtaacgggg gaactcgcgc
     6541 gtgtagtgct cgatcagccc ctccaccgtc cagtccggaa agaagcggcg gcggtcggcc
     6601 tcgacgtcct gtccccagca gaccgagaac tcctcctcgg tgagcggccg ctggccgtgc
     6661 gcttccagcg tgtcgttcag gagccgccac cagcactgga aggagtcgac gagcacgccg
     6721 tcgaggtcga agacga
//
LOCUS       DASSLZ010000127         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_800243, whole genome shotgun sequence.
ACCESSION   DASSLZ010000127 DASSLZ010000000
VERSION     DASSLZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800243"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFM00_04715"
     CDS             complement(<1..558)
                     /locus_tag="VFM00_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET7497581.1"
                     /translation="MSAEIITIGTELLHGLKRNTNADRILERLAEIGVEVIFQTTVGD
                     DTARLSEAFRAAGHRARFVIATGGLGPTPDDLTRKVIATVFRRRLVLDESVLAHIRAR
                     FKSRGIDMPAINEAQALVPRGARVIENPRGTAPGLHFDHQETDYFALPGVPAEAEAML
                     TQYVIPFIKAANLGPGISRRVIRTIG"
     gene            complement(572..1081)
                     /locus_tag="VFM00_04720"
     CDS             complement(572..1081)
                     /locus_tag="VFM00_04720"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET7497582.1"
                     /translation="MRSAAVLLATAFGIGRFPVAPATAASLVVALALALLAHLAPSSL
                     APAPLLIAILALLPLAVWSSGEAEKELGTDAKPIVIDEVIGMMVSVWGGLGMGALGGT
                     ARLEGPRAWIYLAAAFLLFRLFDILKPFGIRRSQALPGGYGVVVDDLLAGILTNLVLR
                     ILLAMGAPL"
     gene            complement(1096..1674)
                     /locus_tag="VFM00_04725"
     CDS             complement(1096..1674)
                     /locus_tag="VFM00_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HET7497583.1"
                     /translation="MSLINYSSREINCKIVYYGPGLCGKTTNLQHIYSRVPQETRGKM
                     ISLATEMDRTLFFDFLPLELGQIRGFRTRFHLYTVPGQVYYNASRKLILKGVDGIVFV
                     ADSQIDRFDANVESLMNMHDNLSEHGLATEKVPLVMQYNKRDLPRVVSVADLEKELNP
                     SQAPYFEAVALRGAGVFDTLKVACKLVLKTLG"
     gene            complement(1691..2194)
                     /locus_tag="VFM00_04730"
     CDS             complement(1691..2194)
                     /locus_tag="VFM00_04730"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET7497584.1"
                     /translation="MVKGNWALFEEDFYSISLILQTLMRSAGSRSVMLIDKTGQLINS
                     IGEPPGFDVTSFSSLAAADFAANAQLAEMVGEKDFATLVHQGTNESLYLSLIANRVIL
                     VVLFDKKTSLGLVRLKARRASDELIVVLNRLFNKLQYRNEEATVPTLGAEFLAEADSE
                     IDSLFRE"
     gene            complement(2414..>2949)
                     /gene="recR"
                     /locus_tag="VFM00_04735"
     CDS             complement(2414..>2949)
                     /gene="recR"
                     /locus_tag="VFM00_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET7497585.1"
                     /translation="SAQRIAFHMLRTSEADARRLAEAILDLKSKVTDCEICGNVTETQ
                     PCALCADTRRDPSVLCVVEQPMDVLALERTGEFRGRYHVLKGALSPVDGVGPDQLRLT
                     ELLRRVKEGNVREVIVATNPTAQGEATALYIARLLQSVPDLAVTRIARGVPMGSDLEF
                     SDQVTLARALTGRKEIG"
BASE COUNT          489 a          971 c         1022 g          467 t
ORIGIN      
        1 cccgatcgtg cggatcacgc ggcgcgagat cccgggcccg agattggccg ccttgatgaa
       61 ggggatcacg tactgggtga gcatcgcttc ggcctcggca gggacgccgg ggagcgcgaa
      121 gtaatcggtc tcctgatggt cgaagtgcag gccgggagcc gtgccgcgcg ggttttcgat
      181 cacgcgcgcg ccgcgcggga ccaaggcctg cgcctcgttg atcgcgggca tgtcgatccc
      241 ccgcgacttg aaccgcgcgc ggatgtgggc cagcaccgat tcgtcgagca ccaggcggcg
      301 gcggaacacc gtcgcgatca ccttgcgcgt gaggtcgtcc ggggtcgggc ccaggccgcc
      361 ggtcgcgatg acgaagcgcg cccggtgccc cgcggcgcgg aacgcctcgg agaggcgcgc
      421 cgtgtcgtcg ccgaccgtgg tctggaagat cacctcgacc ccgatctcgg ccagccgctc
      481 gaggatccgg tcggcgttcg tgttccgctt cagcccgtgc aagagctcgg tgccgatggt
      541 gatgatctcg gcgctcacgg aatggctcct ttcacagcgg ggctcccatc gcgagcagga
      601 tccgcagcac cagattcgtc aggatccccg cgagcaggtc gtccacgacg acgccgtagc
      661 cacccggcag cgcctggctc cgccggatcc cgaacggctt caggatatcg aagagtcgaa
      721 agagcaggaa tgccgcggcg aggtaaatcc aggcccgggg cccctcgaga cgggcggtcc
      781 ccccgagcgc ccccatgccg agtccgcccc acaccgacac catcatcccg atcacctcgt
      841 cgatgacgat cggcttggcg tcggtcccca gctccttctc cgcctcgccg ctggaccata
      901 ccgcgagcgg caggagagcc aggatcgcga tcagcagcgg ggccggcgcg agcgacgagg
      961 gggcgaggtg ggcgaggagc gccagggcga gcgccacgac cagcgaggcg gccgtcgcgg
     1021 gggcgaccgg gaagcgcccg atgccgaacg ccgtggcgag gagaacggcg gcgcttcgca
     1081 tgccggggcc gggccctagc ccagcgtctt cagcacgagc ttgcaggcca ccttcagcgt
     1141 gtcgaagacg cccgcgccgc gcagcgcgac cgcctcgaag tacggcgcct ggctcggatt
     1201 cagctccttc tcgaggtcgg ccacggaaac cacccgcggc aggtcgcgct tgttgtactg
     1261 catcaccagc ggaaccttct cggtggccag gccgtgctcg gagaggttgt cgtgcatgtt
     1321 catcaggctc tcgacgttcg cgtcgaagcg gtcgatctgc gaatcggcca cgaagacgat
     1381 cccgtccacc cccttcaaga tgagcttccg gctggcgttg tagtagacct gcccgggcac
     1441 ggtgtacagg tggaaacggg tgcggaagcc gcggatctgg cccaactcga gcggcaggaa
     1501 gtcgaagaag agggtgcggt ccatctcggt cgcgagcgag atcatcttgc cgcgggtctc
     1561 ctgcggcacg cgggagtaga tgtgctggag gttggtcgtc ttgccgcaga gccccggacc
     1621 gtaatagacg atcttgcagt tgatttcccg ggacgagtag ttgatcagcg acacgaagcg
     1681 atcgtgctcc ttactcccgg aacaggctgt cgatctcgct gtcggcttcc gccaggaatt
     1741 cggcgcccag cgtgggcacc gtggcctcct cgttccggta ctgaagcttg ttgaagagcc
     1801 ggttcagcac gacgatcagc tcgtccgagg cgcggcgcgc cttcaggcgc accagtccca
     1861 gcgaggtctt cttgtcgaag aggacgacca ggatcacgcg attggcgatg agcgacaggt
     1921 agaggctctc gttcgtcccc tggtgcacca gggtggcgaa gtccttctcg ccgaccatct
     1981 cggcgagctg ggcgttggcc gcgaaatcgg ccgccgcgag cgaggagaag gaggtgacgt
     2041 cgaaaccggg aggctcgccg atcgagttga tgagctgacc ggtcttgtcg atcagcatga
     2101 cgctgcgcga tcccgcgctg cgcatcaacg tctgcagaat gagactgatc gaatagaagt
     2161 cttcctcgaa aagcgcccaa ttacccttga ccatcgtcct ggctccttgg acaacgggcc
     2221 cgagacgcgg cacggatcgg ccgcccgccc gcgaagaggg tgccctgccc gtccctcgcg
     2281 aaccctgcgg cgaggccgat gtgcccccac acccatccat ggcgcgacag cctgtcgcgc
     2341 gacttcgagg aattgggcga aacctaccat gttcccccca tgggtcaagg ggaatgtgcc
     2401 ggaaaatttg gggttacccg atctccttcc ggccggtcag ggctcgggcg agcgtgacct
     2461 ggtcggagaa ctcgaggtcg gatcccatcg gcacgccgcg cgcgatgcgc gtgacggcga
     2521 ggtccgggac cgactggagc aggcgcgcga tgtagagggc cgtcgcctct ccctgcgccg
     2581 tgggattggt cgcgacgatc acctcgcgca cgttcccttc cttcacgcgg cggagaagct
     2641 cggtcaggcg gagctggtcg gggccgacgc cgtccacggg cgagagggcg cccttgagca
     2701 cgtggtagcg gccgcggaat tcgcccgtcc gctccagcgc cagcacgtcc atcggctgct
     2761 ccacgacgca caggacggac ggatcgcggc gggtgtcggc gcacagcgcg cagggctggg
     2821 tctcggtgac gttgccgcag atctcgcagt cggtgacctt ggatttcaga tcgaggatcg
     2881 cttcggccag gcggcgcgca tccgcctcgg acgtgcgaag catgtggaag gcgatccgct
     2941 gcgccgact
//
LOCUS       DASSLZ010000128        18147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_929512, whole genome shotgun sequence.
ACCESSION   DASSLZ010000128 DASSLZ010000000
VERSION     DASSLZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 18147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18147
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929512"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VFM00_04740"
     CDS             complement(<1..880)
                     /locus_tag="VFM00_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497586.1"
                     /translation="MAAPHAPSPVRFATFAALVALLLVAPGARAQYMYLDSNGDGAHT
                     AADVLPPTGTTTVDVWLSSDHNRDGSAAVCFTGSQPFTISSYEFALRATDGHVAFSDF
                     VNHATLPTTLFAGGSTTEFYAAQFGIIAMPAGLYRLASISVAVTFGTPSLQVVTDAPT
                     LDHVAPGPPETAFGSQCGGAGFDNTIILGRDWFDVDGLVPGPPSTGGAPGLDPVTDMT
                     VDVGDVAAQALRATDPDGDPIAFALESGPAFADVTTEDAGRGIAVGTLHAVPHADDTG
                     LHSATVAASDGALSARG"
     gene            complement(984..2087)
                     /locus_tag="VFM00_04745"
     CDS             complement(984..2087)
                     /locus_tag="VFM00_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET7497587.1"
                     /translation="MPRATRDLNLEIAGQLYDLAFIHASPHGRIAYKRAAQAVLRLEE
                     PLDAVVAAGSLRQVPYIGPASERVILESLEHGTSSTVERAVAASGKAAEVEAAHAHRT
                     NFLSRAEALHLIRSRSGAVGIEDYRGDLQMHTTWSDGGESIARMAAAARERGYEYIGV
                     SDHSYGLRIARGMSMKDAARQRAEIETLNRRWKGAFRVIQGIEANIPAEGGVDMTPEE
                     IATFELLLAAPHSKLRKAEDQTERMLATVRHPGVHILAHPRGRMYSRQGVLARWDEVF
                     EEAAKRAVAIELDGDPYRQDLDFALAKRALAAGCLFALDSDAHSGAELVYAEYAIAHA
                     RRAGIPASRVINTWPAAKLLDWARRKSRRRSRS"
     gene            2146..2622
                     /locus_tag="VFM00_04750"
     CDS             2146..2622
                     /locus_tag="VFM00_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET7497588.1"
                     /translation="MHALMDELRNGLMVDWAQLVRIAVRLLLSAGLGAVVGLQREQTG
                     KPAGLRTHMLVALGATLFVLAPLEMDVSMSDIGRVIQGVATGIGFIGGGAILKLSAER
                     EVRGLTSAAGIWITAAAGVAVGLGRLAIAAVSILLTWIILAIIGRAERRIEKNRGL"
     gene            complement(2628..3050)
                     /locus_tag="VFM00_04755"
     CDS             complement(2628..3050)
                     /locus_tag="VFM00_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET7497589.1"
                     /translation="MAVFLFKTEPTSYSFDDLSRDKTTVWDGVSNALALKHLRSVQKG
                     DTIAIYHTGDEKQVVGLATASSDPFPDPKQKDPKRVVVRLKADRALPFPVPLATFRTD
                     AALKTADLVRLPRLSVMPLTQAQYQRLLERSGLSTGKS"
     gene            3102..3374
                     /locus_tag="VFM00_04760"
     CDS             3102..3374
                     /locus_tag="VFM00_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010495249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HET7497590.1"
                     /translation="MCRNIKTLFNFDPPATDEEIRAASLQFVRKLSGFTKPSAANEAA
                     FQRAVDETAVVAKRLLDSLVTNATPRDREIEAERARARAAVRFGKG"
     gene            3552..4067
                     /locus_tag="VFM00_04765"
     CDS             3552..4067
                     /locus_tag="VFM00_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497591.1"
                     /translation="MFSQAAAAAALLALCAMLPGTASAHASVPPPSLASDPLATTDAP
                     EWHLLFGQAYLDTVTASTAAARIASVAALESDHWEMEPSGALAHLISQWKPVHHLIFR
                     MFSGKAFGRVLIDVKPLSGRRAQIRFQGVLASHRDIEHNPARGWAEHAYASAVRVWQK
                     EMHDDLARGVP"
     gene            4076..5617
                     /locus_tag="VFM00_04770"
     CDS             4076..5617
                     /locus_tag="VFM00_04770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7497592.1"
                     /translation="MKVSVVIPAYNEEVLLPACLDSLSRQDYPGEIEIIVVDNGSTDG
                     TAAVARSRGVRVVTETERGYSRALASGFAAATGAILASTDADTVVPPFWISRLVREYV
                     RRPDVVAVGGEIVFCEPNLGARLFTRGLLPLLNRADRSRPEGPHLWGANFSVRRGAFE
                     AAGGWNLDFNLQVDTELSERLRAHGRVVLLEDVPVYTSCRRWNRMLGRSLFVYATNFL
                     SLELRGRPLWRSFPNVREAALSAPVRGPAQRPLAWAMSVAVAVLAGIGVYDMLSPWSS
                     AFGGTHWAGPGRDRVVALTFDDGPNEPYTSEVLAILRRERVRATFFLIGDRVRREPGI
                     AAEIAREGHVVGNHSETHPFALALETPERIRSELDRAERQIHAATGIYPRLFRPPQGL
                     RSPWLMSVAESDSLDTVTWDDSPVDWNPLTARQLSDRVVAAAHPGAIILLHDGLNLVP
                     GADRRVVVSGLSSIIERLRAEGYRFVTVPDLLREPSSLSRWPARYRTTTLPASRGAQA
                     RRLAS"
     gene            complement(5621..6568)
                     /locus_tag="VFM00_04775"
     CDS             complement(5621..6568)
                     /locus_tag="VFM00_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497593.1"
                     /translation="MSHPRFVRPVPFLALAAVLLAFSLPMPVCAADAAGPTWQEAGAA
                     YQKEDWKAAAAAYGAIAKREPGNGRAWFRLGTSYLKLGNLKDAIPAYLKSDAIGENPL
                     VRYNLACVYTRMGDSAQAFVWLEKAIAAGYRSTESLKTDADLQPLRGTSHFASVVQRV
                     EWNERPCAHLAESRQFDFWVGQWDVRAPEGSPAGQSSITVENGDCWIHEHWTGGAGGK
                     GESFNYYNPTTKTWHQTWVDDQGGIAEFDGTFHDGAMRLEGYRQGLKNERIPARLTLT
                     PLADGTVRQLGENSSDGGKTWTVLYDLLYSKKKDAASAG"
     gene            complement(6686..7477)
                     /locus_tag="VFM00_04780"
     CDS             complement(6686..7477)
                     /locus_tag="VFM00_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7497594.1"
                     /translation="MKYALISDIHGNLPALEAVLADLETRSEIDATYHLGDLVGYGPW
                     PDEVVARLRFRGIAGVAGNYDSTTATNYMHCGCRYENPRQEELAHQSYAWTLTHIHHD
                     TRHYLAGLPFRLDLRVMGGHVPEGPRIVLVHASPVANTLYWTEDRPDSFCLQMAKIAG
                     MQPGDIIAFGHTHRPWYREIAGMHFVNTGSVGRPKDGNWQAGYVLLEVGDAAPHVTFV
                     RVDYDIERAMRAIRESALPDEFAEDLRTGGSTAPSNEYVTIETRP"
     gene            complement(7474..8571)
                     /gene="arsB"
                     /locus_tag="VFM00_04785"
     CDS             complement(7474..8571)
                     /gene="arsB"
                     /locus_tag="VFM00_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HET7497595.1"
                     /translation="MNRFERYLTVWVALCMAAGILLGKAAPDFVGALRGLEFGRDSRI
                     NLPIAILIWLMIYPMMLRVDFASIAGVRRRPRGILVTLFVNWLVKPFSMALLGWVFFK
                     HLFLPILGPALADQYVAGTIILAAAPCTAMVFVWSYLTDGDPAYTLVQVSLNDLIMLV
                     AFAPIVSLLVAGASALTVPFGVLLYSVVAFIVIPLGLGSLSRVLLLRAKGAAWFERAF
                     LPVFRPITTLALLATLILIFAFQADNITSRWFHVLLIAVPILIQVYLNAGLAYGLMRW
                     LRVPYNVAAPGALIGASNFFELAVAVAITLFGPGSGAALATVVGVLVEVPVMLSVCAA
                     CNRTRHWFPEGGPAEAARIPALKPAAPGAAP"
     gene            complement(8568..8984)
                     /locus_tag="VFM00_04790"
     CDS             complement(8568..8984)
                     /locus_tag="VFM00_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7497596.1"
                     /translation="MRDLELALKAAGDPTRTRILKLLESGGLCVCQVQAVLALAPSTV
                     SKHLAILKHAGLVRDRREGKWIEYALTPRSPNAYAHPLLAMLRGALDKDPAVLRDRKR
                     LRAVKAIPVSELCAADPGRSHRAAGAKALPLRPRPR"
     gene            9043..9726
                     /locus_tag="VFM00_04795"
     CDS             9043..9726
                     /locus_tag="VFM00_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7497597.1"
                     /translation="MGQVALVTGSTRGIGQETARQLAARDYTVVVTGRSLRAASEAAA
                     GIGARAVARALDVSDPKSVAALGAWIASAIGSLDVLVNNAAILLDEGKSILTLDPQTF
                     EATMRTNVLGALLVTQAMAPLLRRARAPRVVNVSSGAGQISSMTTYAPGYSISKAALN
                     AVTVLLAAALPNARVNCVDPGWVRTDMGGPNATRGVAEGAETIVWLATLPANGPTGGF
                     FHDRKRIAW"
     gene            complement(9754..10863)
                     /locus_tag="VFM00_04800"
     CDS             complement(9754..10863)
                     /locus_tag="VFM00_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET7497598.1"
                     /translation="MSDPRVERLADLLVNYSIAARPGQRVAIQGSTLAEPLIRAVYAE
                     VLKAGAYPFVLAQIPGLDETFYRHASDDQLQHVPPPFRQIMETYEGFIAIWGAANTKS
                     LSNVPPQKMALNQKAAGDVTRIFMQRAAAKELRWVGTLFPTPASAQDAEMGLTEFEEL
                     VYGACLPDRDDPIGYWKRFSGWQQRIVDWMRGKKEIRVLGPDTDLRLSIEGRPFINCD
                     GRANMPDGEVFTSPVEGSVEGRVRYTYPAVYRGREVTNVRLRLEKGRVVEAKADKGED
                     FLNQTLDIDAGARVPGEFAIGTNKGVTRFMRNTLFDEKIAGTFHMALGASIPESGGTN
                     VSAVHWDMVCDLRDGGEIRVDGELLHQNGKFVIDF"
     gene            complement(10943..11656)
                     /locus_tag="VFM00_04805"
     CDS             complement(10943..11656)
                     /locus_tag="VFM00_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497599.1"
                     /translation="MRRLSDEAPESPVIDIHEKQWTIRNWVMGGLVVAVLVLSGLIVA
                     GIRVNEAREQRNRAVAAGTSGKFVADIQDLMQRVNEKTTDSRIAGIAAAVAESSLAQA
                     GVKIARMSANLAQLSKEQEELTATLGALQQQSARLSAENDALRADLVASNNRVVAFQA
                     QRVADSLALAEQNASLNRRLTSVADQTADVDRRVGKSNSTMRGLGGVTAANFAVGLIH
                     LLGTKQTSEVTPSTATVGR"
     gene            11905..13440
                     /locus_tag="VFM00_04810"
     CDS             11905..13440
                     /locus_tag="VFM00_04810"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HET7497600.1"
                     /translation="MNALESLKARLLEASDLEYAASLLHWDQTTYMPPGGAEARGRQI
                     ALLSKLAHERFTAPETGKLLEAAERETASLPYDSDEASLARATRRDWDLQIRRPSSLV
                     AELYEQAARSYQAWIAARPANDFASVRPHLERTLELSRRMADCYPGYDHIADPLIAFA
                     DYGMKAESIRTLFAELRRRLVPLAQAITSRPLADNSCLHRRSRLEDQTAFGIDVIRRF
                     GYDFQRGRQDFTRHPFMTKFSLGDVRITTRVNEDDLTDMLFSTLHECGHALYEQGIDP
                     GYEGTPLASGTSSGVHESQSRLWENLVGRSRGFWEFFFPRMQAAFPEQLRGVSLDAFY
                     KAVNRVERTLIRTDSDEVTYNLHVMLRFQLELDLLEGKLAVADLPRAWHERFEADMGL
                     HVPDDRNGVLQDVHWFSGRIGGVFQGYTLGNILSAQFFDTAVKAHPAIPQEIQNGEFG
                     TLHGWLRENIYRHGAKFTPAELVRRVTGRDLTVEPYMDYLWRKFGPLYGLRKEELGGV
                     SVG"
     gene            13437..14201
                     /locus_tag="VFM00_04815"
     CDS             13437..14201
                     /locus_tag="VFM00_04815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7497601.1"
                     /translation="MSPRRKREWFDDDGFWRDLYPFMFPETRFEAAVLDTPKLIRLAR
                     PRGRSVLDLACGPGRFSIAFAKRKYRVTGVDRTRYLLDRARARARTAKVQVEWIRQDM
                     RDFVRPDAFDLALSLFTSFGYFQDKSEDVRVLRQVLESLRPGGALVVEVMGKEQIAGI
                     FAPVSADKLGDALLVQRREIFDDWTRIWNEWTLIRGGRAKTYEFTHTIYSGQELRERF
                     EQAGYSDVKLYGNLEGEPYQRRSPRLVAVGWKGSRQ"
     gene            14280..14861
                     /locus_tag="VFM00_04820"
     CDS             14280..14861
                     /locus_tag="VFM00_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7497602.1"
                     /translation="MRRALILAAFVLMSFVPVQAFAAWGLGANLGMTLHNPAETGSDN
                     VTLIGFPSQASVFTSLRPGLRVGFAGDSSPHEVYLDVSLDHEASGGDSFTAVRLGGNY
                     QYNFSGTSFRPYLTAGLGVFSEHADFSSSTSATSMTFGGGAGVGFPIGDQAGRLRLEA
                     RYDKLQEGKDNGDVVIGEAGITSLLFGFDLWLR"
     gene            14873..15826
                     /locus_tag="VFM00_04825"
     CDS             14873..15826
                     /locus_tag="VFM00_04825"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HET7497603.1"
                     /translation="MRTKPPIAPILVLGLFAGLLAALTSCGGGGDAQPALTAAEADTL
                     MKSYAKDRSDTETWLKSSPTSYLATTQRQDFGDRTTMTIGSAAGSDVRIEDPAVKPRH
                     LRVTVEGDSFHVEGVDRGATFEILKDSTTATSATVPPSSIKVARFTLRLSHQRFPAII
                     VFDPQSPRYQLYKGVKYFPPDLAYHIVAVLTPNPRPDTTIILSTRGNRRRAVRVGWFD
                     FKLKGTPVRLEANRLLEPGVGEKDMSLFFTDATTGKESYGVGRYLDPVPRPDGRYVLD
                     FNNCYNPACAYSEHYNCPIPPAENHLKVAIAAGEMDSHYMH"
     gene            15947..16846
                     /locus_tag="VFM00_04830"
     CDS             15947..16846
                     /locus_tag="VFM00_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497604.1"
                     /translation="MSRNPFSSRFRRFLFIFVLVASVAIASPASARIGIGNPVKKMKE
                     KIQNKAEGKAAPEATEAAGPPTFDDVTLELTDARIAGIIDAYQKSKKIAADRAPLNAK
                     RNQLMDERNKLSDKEGDKMRELREKRGDMEQCYQSGYRAAGDKKAEDYKNRALSDPVL
                     RDKFTKAAMQYNEAAAKGDSAAIQKLQSILTGEILPSKEDSAAVRAKCGPMPPKSAAE
                     IKDEQLSKDIDAVDEQIRKIDEEVSKVGNGGMNPQQWGTALERIQLYVQSKKAKKEPK
                     MLTDQEIRAVEKRLAELEEAMGI"
     gene            16849..17802
                     /locus_tag="VFM00_04835"
     CDS             16849..17802
                     /locus_tag="VFM00_04835"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HET7497605.1"
                     /translation="MRAPRAMAVAAAFVVACVASPLAAEGPATLAFDQHGTWHTFWRS
                     DQAPARWSGPDSMLSRSLVWKRLARGSDWASVRLACAAPVWRARVIVARLDPREVALS
                     LAMDLTRAEGRPAWSIDRAPEDAILAVNAGQFIRTMPWGWVAMDGRQRLRPGFGPLSS
                     AVAVDASGRVRWAHGDSLLDATGVVAGFQSYPTLLEGNGVVPAMLRSLNHLVSLTHHD
                     SRLALGQTRDGRLLVVMTRFDAMGEEAGGFPLGPTTPEMAAIMGALGASDAVMLDGGI
                     SAQLMLRDGNGKTLRWPGVRKVPLALIARSRVAAPAVRASK"
     gene            17833..>18147
                     /locus_tag="VFM00_04840"
     CDS             17833..>18147
                     /locus_tag="VFM00_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HET7497606.1"
                     /translation="MIAFHSSRHEEHAPPFEYHRGEIVPCFETPARIRIVCDELAARG
                     HRLREPDQDSASLLPLVHTPRYLAFLGTAWDQWLETDRGRAEVQPFPAVWPVRTLRGD
                     VER"
BASE COUNT         2804 a         6212 c         6281 g         2850 t
ORIGIN      
        1 tcccgcgagc ggacagcgca ccgtcgcttg cggccaccgt ggccgagtgg aggccggtgt
       61 cgtccgcgtg cggcacggcg tgcagcgttc ccacggcgat gccgcgaccc gcgtcctcgg
      121 tggtcacgtc ggcgaaggcc ggcccggatt ccagggcgaa cgcgatcggg tctccgtcgg
      181 ggtctgtggc ccgcaacgcc tgagccgcca cgtcgccgac atccaccgtc atgtccgtga
      241 ccggatccaa gcccggggcg cctcctgtcg aggggggacc ggggacgagc ccgtccacgt
      301 cgaaccagtc gcgtccgagg atgatcgtat tgtcgaagcc ggcgccgccg cactgggagc
      361 cgaacgccgt ctcgggcggt ccgggcgcca cgtgatccaa cgtgggggcg tccgtgacga
      421 cctggagcga aggcgtgccg aacgtgaccg ccaccgagat cgacgcgagc cggtacaggc
      481 ccgccggcat cgcgatgatg ccgaactgcg ccgcgtagaa ctcggtcgtg gaaccccccg
      541 cgaagagcgt ggtcgggagg gtcgcgtggt tcacgaagtc gctgaacgcg acgtgaccgt
      601 cggtggcgcg gagcgcgaat tcgtacgagg agatggtgaa gggctggctg cccgtgaagc
      661 agacggccgc ggagccgtcc cgattgtggt cggaggagag ccagacgtcc accgtcgtgg
      721 ttcccgtggg gggcagcacg tcggcggcgg tgtgcgcgcc gtcgccgttc gaatcgaggt
      781 acatgtactg agcgcgcgcg cccggcgcca ccagaaggag ggcgacgagc gcggcgaacg
      841 tggcgaagcg aaccggggag ggtgcgtgtg gggcggccat cgggatctct cctcggccgg
      901 tccgcggacg caccggcctc gtgagggcac gtgggggatg aatgttcatt ctagcaccgg
      961 gcggtgcggc gccccagggc cgactacgag cggctccgtc gcctcgactt gcgacgggcc
     1021 cagtccagca gcttcgcggc gggccacgtg ttgatcacgc gcgaggcggg aatcccggcg
     1081 cggcgtgcat gcgcgatcgc gtactcggcg tagaccagct cggcgccgga gtgcgcgtcg
     1141 ctgtccaggg cgaacaggca gcccgccgcg agcgcgcgct tggccagcgc aaagtccagg
     1201 tcctggcggt agggatcgcc atccagctcg atggcgacgg cgcgcttcgc cgcctcctcg
     1261 aagacctcgt cccagcgcgc gagcacgccc tggcgggagt acatgcgccc gcggggatgc
     1321 gcgaggatgt gcacgccggg gtggcgcacc gtggcgagca tgcgctccgt ctgatcctcc
     1381 gccttccgca gcttcgaatg gggcgcggcc aggagcagct cgaacgtggc gatctcctcg
     1441 ggcgtcatgt ccacgccgcc ctcggccggg atgttggctt cgatcccctg gatgacccgg
     1501 aaggcgccct tccaccggcg gttcagcgtc tcgatctcgg cgcgctggcg cgcggcgtcc
     1561 ttcatgctca tcccgcgcgc gatgcgcagc ccgtacgagt ggtcgctcac gccgatgtac
     1621 tcgtagccgc gctcccgggc ggcggccgcc atgcgggcga tgctctcgcc gccgtcgctc
     1681 cacgtggtgt gcatctggag gtcgccccgg tagtcctcga tgccgacggc ccccgatcgg
     1741 gatcggatca ggtggagcgc ctcggcccga ctcaggaagt tcgtccggtg cgcgtgggcc
     1801 gcctcgacct cggcggcctt gccgctcgcc gctaccgcgc gctcgacggt ggaactggtc
     1861 ccatgctcca gcgactcgag gatgacccgt tcgctggcgg gaccgatgta gggcacctgg
     1921 cgcaggctcc cggccgccac gacggcgtcg agaggctcct ccaggcgcag caccgcctgg
     1981 gccgcccgct tgtaggcgat ccggccgtgc ggtgaagcgt ggatgaaggc caggtcgtag
     2041 agctggcccg cgatctcgag attgaggtcg cgcgtagccc gcggcatggg gtcagtctat
     2101 gatcccggcg gcctgtcgtg aacgtcgaga tcgggagggt ggggaatgca cgcgctgatg
     2161 gatgagcttc gaaacggatt gatggtggac tgggcgcagc tcgtgcggat cgcggtgcgg
     2221 ctgctcctct ccgcggggct cggggccgtg gtcgggctcc agcgggagca gaccgggaag
     2281 cccgcgggac tgcggaccca catgctggtc gccctcggcg cgaccctctt cgtgctcgcc
     2341 cccctcgaga tggacgtttc gatgagcgac atcggccggg tgatccaggg cgtggcgacg
     2401 ggcatcgggt tcatcggcgg aggggccatc ttgaagcttt ccgccgagcg cgaagtgcgc
     2461 gggctgacgt cggccgcggg gatctggatc accgccgcgg ccggcgtggc ggtggggctg
     2521 ggccgtctcg cgatcgccgc ggtgagcatc ctcctcacct ggatcatcct ggccatcatc
     2581 gggcgcgccg agcggcgcat cgagaagaac cggggactgt agaagagtta cgacttcccg
     2641 gtggagagcc ccgagcgctc gagcaggcgt tggtactgcg cttgggtcag cggcatcacc
     2701 gagagccgcg gaaggcggac gagatccgcg gtcttgagcg cggcgtcggt ccggaacgtg
     2761 gcgagcggaa cggggaaggg aagggcccgg tccgctttca agcggaccac gacgcgcttg
     2821 ggatccttct gcttgggatc gggaaaggga tcggaggagg cggtcgcgag gccgaccacc
     2881 tgcttctcgt cgccggtgtg gtagatggcg atggtgtcgc ctttctggac agagcgcagg
     2941 tgtttcaagg cgagcgcgtt ggacaccccg tcccagaccg tcgtcttgtc acggctcagg
     3001 tcgtcgaagg agtacgacgt cggttcggtc ttgaagagaa agacggccat ccccagactc
     3061 tagcgacacc cacccggctc ccgcaacgaa agaggatggc catgtgccga aacatcaaga
     3121 ccctgttcaa cttcgatccg cccgccaccg acgaggagat ccgggcggcc tcgctccagt
     3181 tcgtccggaa gctctccggt ttcaccaagc cctccgccgc gaacgaggcg gcgttccagc
     3241 gtgccgtgga cgagacggcc gtggtcgcga agcgtctcct cgattccctc gtgacgaatg
     3301 ccacccctcg cgaccgcgag atcgaggcgg agcgcgcccg cgcgcgggcg gccgtgcgat
     3361 tcgggaaggg ctgagcccgg cgcaggcact ggtccctgcg aaccgttccg cgagggcggg
     3421 gttcttgcag gcaccatcct ggggcgccgt gccggcgccc cccgacgtgc ggcaccaccc
     3481 tggagcggcg gcgatcttcg atgaccggcg ctttggcagt tcccggcccc gcttctcgat
     3541 tcgcgggtgg cgtgttctcg caagccgcgg ccgcagcggc gctgctcgct ctctgcgcca
     3601 tgctccccgg aaccgcttcg gcacatgctt cggttcctcc gccttccctt gccagcgacc
     3661 cgctggccac gaccgacgcg ccggagtggc atctgctctt cggccaggcc tacctcgata
     3721 ccgtcacggc atcgacggcc gccgcgcgca tcgcctcggt cgcggcgctc gagagcgacc
     3781 actgggagat ggagccctcg ggcgcgctcg cgcacctcat ctcgcagtgg aagccggtcc
     3841 accacctgat ttttcggatg ttctcgggca aggcgttcgg gcgcgtgctc atcgacgtga
     3901 agcccctgtc cgggcggcga gcccagatcc gtttccaggg cgtgctggct tcccatcgcg
     3961 acatcgagca caacccggct cgggggtggg cggagcacgc ttacgcttcg gcggtgcgcg
     4021 tctggcaaaa ggaaatgcac gatgacctgg cccggggggt cccctgagca cgcccatgaa
     4081 ggtcagcgtg gtgatccccg cgtacaacga ggaggtcctt cttcccgcgt gcctcgattc
     4141 gctgagccgc caggactacc cgggcgagat cgagatcatc gtcgtggaca acggcagcac
     4201 cgacgggacc gcggcggtgg cgcgatcgcg aggcgtgcgc gtcgtcaccg agacggagcg
     4261 gggctacagc cgggcgctgg cgagcgggtt cgccgccgcc accggcgcga ttctcgcgag
     4321 cacggacgcg gacacggtgg tgcccccctt ctggatctcg cgcctggtgc gtgagtacgt
     4381 ccggcggccc gacgtcgtcg ccgtgggcgg ggagatcgtc ttctgcgagc cgaacctcgg
     4441 ggcgcgcctc ttcacccgcg gcctgctgcc gctgctgaac cgcgcggacc gaagccggcc
     4501 cgaggggccg cacctctggg gagccaactt ctcggtgcgc aggggcgcct tcgaggcggc
     4561 cggcggctgg aacctcgact tcaacctcca ggtcgacacg gagctgtcgg aacgcctgcg
     4621 cgcgcacggc cgggtcgtgc tgctggaaga cgtgccggtc tacacgtcct gccggcgatg
     4681 gaaccggatg ctcggccgga gcctcttcgt ctacgccacg aatttcctct cgctggagct
     4741 gcggggccgg ccgctctggc ggtccttccc gaacgtgcgg gaagcggcgc tgtcggcgcc
     4801 ggttcgggga cccgcccagc ggccgctcgc ctgggccatg tcggtcgcgg tggcggtgct
     4861 cgcggggatc ggcgtctacg acatgctctc gccctggtcc agcgccttcg gcggcaccca
     4921 ctgggccgga cccgggcggg accgcgtcgt ggcgctgacg ttcgacgacg gtcccaacga
     4981 gccctacacc tcggaggttc tcgcgatcct gcgacgcgag cgcgtgcgcg cgacgttctt
     5041 cctgatcggg gaccgggtcc gccgggagcc gggcatcgcg gcggagatcg cgcgcgaggg
     5101 gcacgtcgtg gggaaccact cggagacgca tccgttcgcg ctcgcgctcg agacgccgga
     5161 acggatccgt tccgagctgg accgcgcgga gcggcagatc cacgccgcga ccggcatcta
     5221 tccgcgcctt ttccgtccgc cccagggact gcgttccccc tggctcatga gcgtcgccga
     5281 gagcgactcc ctcgacacgg tgacctggga cgattcgccg gtggactgga atccgctcac
     5341 ggcgcgccaa ctctccgatc gcgtcgtcgc cgccgcgcat cccggggcga tcatcctgct
     5401 ccacgacggc ctcaacctcg tgcccggcgc ggaccggcgc gtcgtggtca gcgggctctc
     5461 ctcgatcatc gagcgcctgc gggccgaggg ctatcggttc gtgaccgtgc ccgacctgct
     5521 ccgggagccg tcctcgcttt cgcgctggcc cgcccgctat cggaccacga ccctgcctgc
     5581 ttcacgggga gcccaagccc gccgcctggc atcctgacgc ctagcccgcg ctggcggcgt
     5641 ccttcttctt cgagtagagc aggtcgtaga gcacggtcca cgtcttcccg ccgtcggagg
     5701 agttctctcc gagctggcgg accgtgccgt ccgcaagcgg cgtcagcgtc agacgcgcgg
     5761 ggatccgctc gttcttcaag ccctgccggt atccctccag gcgcatggcg ccatcgtgga
     5821 acgtgccgtc gaactcggcg attcctccct ggtcgtccac ccacgtctgg tgccacgtct
     5881 tcgtggtcgg gttgtagtaa ttgaagctct cgcccttgcc tccggcgccg cccgtccagt
     5941 gctcatggat ccagcagtcg ccgttctcca cggtgatgga gctctgtccg gccgggctcc
     6001 cctccggggc acgcacgtcc cactgtccga cccagaagtc gaactgccgg ctctcggcga
     6061 ggtgagcgca ggggcgctcg ttccactcca cgcgctgtac gaccgaggcg aaatggctcg
     6121 tgccgcgaag cggctggagg tcggcgtcgg tcttcaacga ctcggtggag cggtagcccg
     6181 ccgcgatcgc cttctcgagc catacgaacg cctgcgcgct gtctcccatc cgcgtgtaga
     6241 cgcacgccag gttgtaccgg accagcgggt tctccccgat cgcgtcggac ttgaggtagg
     6301 cggggatcgc gtccttcagg ttgccgagct tgagatagct ggtgcccagg cggaaccagg
     6361 cgcggccgtt gcccggctcg cgcttggcga tcgccccgta ggcggccgcg gcggccttcc
     6421 aatcttcttt ctgatacgcg gctcccgctt cctgccaggt cgggccggcc gcatcggcgg
     6481 cgcaaaccgg catcgggagc gagaaggcga ggagcacggc ggcgagcgcg aggaagggga
     6541 ccggccggac gaagcgcgga tggctcacga aaagaccctc tccggcggcc ggcggcgccc
     6601 gggcagagcc ggggtcacgt ggcggagggg gtacgcctga tcctagatca ggccggcccg
     6661 aacggtaatg gaaatgtgcg gggggtcatg ggcgtgtctc gatcgtgacg tattcgttcg
     6721 agggagccgt gctcccgccg gtccgcagat cctcggcgaa ctcatcgggc agcgcactct
     6781 cgcggatggc acgcatggcc cgctcgatat cgtagtcgac ccgcacgaac gtgacgtgag
     6841 gcgccgcgtc acccacctcg aggagcacgt agcccgcctg ccagttgccg tccttcggtc
     6901 ttcccacgga gccggtgttc acgaagtgca tccccgcgat ctcccggtac cacggccggt
     6961 gcgtgtgtcc gaacgcgatg atgtcgcccg gctgcatccc ggcgatcttg gccatctgca
     7021 ggcagaagga atcggggcgg tcctcggtcc aatagagcgt gttcgcgacg ggagaggcgt
     7081 gcaccaggac gatgcgaggg ccctcgggaa cgtgccctcc catcaccctc agatccaggc
     7141 gaaacggcag cccggcgagg tagtgccggg tatcgtgatg gatgtgcgtg agcgtccagg
     7201 cgtagctctg gtgggccagc tcttcctggc ggggattttc gtagcggcat ccgcagtgca
     7261 tgtaattggt cgcggtcgtg gaatcgtagt tgcccgccac gcccgcgatc ccccggaagc
     7321 gaaggcgcgc gacgacctcg tccggccaag gcccgtagcc gaccaggtct ccgaggtgat
     7381 aggtcgcgtc gatctcgctt cgcgtctcca ggtcggcgag gaccgcctcg agcgcgggga
     7441 gattgccatg gatgtccgag atcagggcgt acttcacgga gccgctcccg gggccgccgg
     7501 cttgagcgcc gggatcctcg cggcctctgc cggcccgcct tcggggaacc agtggcgcgt
     7561 gcggttgcat gccgcgcaca ccgagagcat gacggggact tccaccagga cgccgaccac
     7621 cgtggccagc gcggcgcccg agcccggtcc gaacagggtg atcgccacgg cgaccgccag
     7681 ctcgaagaag ttgctcgccc cgatcagcgc gccgggggcg gcgacgttgt agggaacccg
     7741 gagccatcgc atcaatccgt aggccaggcc ggcgttgagg tagacctgga taaggatcgg
     7801 caccgcgatc aggagcacgt ggaaccagcg gctcgtgatg ttgtcggcct ggaacgcgaa
     7861 gatcaggatc agcgtcgcga ggagcgccag cgtcgtgatg gggcggaaga ccgggaggaa
     7921 cgcgcgctcg aaccatgcgg cccccttggc ccgcaggagg agaacccggc tcaggctgcc
     7981 gaggcccagg gggatcacga tgaaggcgac cacgctgtag agcagcaccc caaacggaac
     8041 cgtgagcgcg ctcgccccgg cgaccagcag gctgacgatg ggagcgaagg ccaccagcat
     8101 gatcaggtcg ttcagcgaaa cctggacaag ggtgtaggcg gggtcgccat cggtcagata
     8161 ggaccagacg aacaccatgg ccgtgcacgg ggccgcggcc aggatgatcg ttccggcgac
     8221 atactggtcg gccagcgcgg ggccgaggat cggcaggaag aggtgcttga agaacaccca
     8281 ccccagcagg gccatcgaga acggcttgac cagccagttg acgaacaggg tgacgaggat
     8341 gcctcggggg cggcggcgga cgccggcgat gctggcgaag tccacccgga gcatcatcgg
     8401 atagatcatc agccagatca ggatggcgat cggaaggttg atccggctgt cccggccgaa
     8461 ttccagcccg cgcagcgcgc ccacgaagtc cggagccgcc ttgccgagga ggatgccggc
     8521 cgccatgcac agcgcgaccc agaccgtgag atagcgctcg aaccggttca tcgggggcgg
     8581 gggcgcaggg ggagcgcctt cgctcccgcc gcgcggtggg agcgtcccgg gtcggcggcg
     8641 cacagctcgg acacggggat cgccttgacg gcgcgaaggc gcttccgatc gcgcagcacc
     8701 gccgggtcct tgtccagagc cccgcgcagc atcgcgagga ggggatgcgc gtaagcgttg
     8761 ggactacgcg gcgtcagcgc gtattcgatc catttgccct cgcgccgatc gcgcaccaga
     8821 cccgcgtgct tgaggatcgc gaggtgcttg gataccgtgg agggggcgag ggccaggacg
     8881 gcctgcacct ggcagacgca gagcccgccc gactcgagaa gcttgaggat ccgggtgcgc
     8941 gtggggtcgc cggccgcctt gagcgcgagt tccaggtcac gcatgcgaaa cgcctccgtt
     9001 tcgtcaattc gtcacatggc gaattataaa ggggacgaac cgatgggtca agtggcgttg
     9061 gtcacggggt cgacgcgggg gatcggtcag gagacggcgc gccagctcgc ggcgcgggac
     9121 tacaccgtgg ttgtcacggg acggagcctg cgcgccgcga gcgaggccgc cgcaggcatc
     9181 ggagcgcggg ccgtagcgcg ggccctggac gtctcggacc ccaagtcggt ggccgcgctg
     9241 ggggcctgga tcgcgagcgc gatcggaagc ctggacgttc tggtgaacaa cgcggccatc
     9301 cttctcgacg aagggaagag cattctcacg ctcgaccccc agaccttcga ggccacgatg
     9361 cgcacgaacg tgctcggggc gctccttgtg acccaggcaa tggcgccgct cctgcgccgg
     9421 gctcgcgctc cgcgggtcgt gaacgtctcc tccggcgcgg ggcagatctc gtccatgacg
     9481 acctacgcgc cggggtactc catctcgaag gcggcgctga acgcggtcac cgttcttctc
     9541 gccgcggcgc ttccgaacgc gcgcgtgaac tgcgtggacc cggggtgggt gcggaccgac
     9601 atgggcgggc cgaacgcgac gcgcggcgtc gcggaggggg cggagacgat cgtgtggctc
     9661 gcgaccctgc cggcgaacgg gcccaccggc ggcttctttc acgaccggaa acggatcgcc
     9721 tggtaggaga cgccgtcgcg cggtcgggtc gcttcagaaa tcgatgacga acttcccgtt
     9781 ctggtggagc agctcgccgt ccacgcggat ctcgccgccg tcgcgcaggt cgcacaccat
     9841 gtcccagtgc accgccgaga cattggtgcc cccggactcg ggaatgctcg cccccagcgc
     9901 catgtggaac gtgcccgcga tcttctcgtc gaagagcgtg ttccgcatga agcgggtcac
     9961 gcccttgttc gtgccgatcg cgaactcccc gggcacgcgc gcgccggcgt cgatgtcgag
    10021 cgtctggttg aggaagtcct cgcccttgtc cgccttcgct tccacgaccc gccccttctc
    10081 cagccggagg cgcacgttcg tgacctcgcg cccccggtac acggcggggt acgtgtagcg
    10141 gacccgcccc tcgacagagc cctcgaccgg actggtgaag acctcgccgt cgggcatgtt
    10201 ggcgcgtccg tcgcaattga tgaacggccg gccctcgatg ctcaagcgga ggtcggtgtc
    10261 ggggccgagc acgcggatct ccttcttccc gcgcatccaa tcgacgatgc gctgctgcca
    10321 gccggagaag cgcttccagt agccgatggg gtcgtcgcga tcgggaaggc aggcgccgta
    10381 gaccagctct tcgaactccg tgaggcccat ctcggcgtcc tgcgcggagg cgggcgtggg
    10441 gaacagggtc ccgacccagc gcagctcctt ggcggccgcc cgctgcatga agatgcgggt
    10501 cacgtcgccc gcggccttct ggttcagggc catcttctgc ggcgggacgt tgctgagtga
    10561 cttggtgttc gccgcgcccc agatggcgat gaacccctcg taggtctcca tgatctgacg
    10621 gaagggaggc ggcacgtgct ggagctggtc gtccgaggcg tggcgataga acgtctcgtc
    10681 gaggccggga atctgggcga ggacgaaggg gtacgcgccg gccttcagca cctcggcata
    10741 caccgcgcgg atcaacggct ccgccagggt gcttccctgg atcgcgactc tctgacccgg
    10801 cctggccgcg atggagtagt tcaccagcag gtcggccagc cgttccacgc gtggatcgga
    10861 catggcgcgg gagcctacac cacgggcagg gccctgtcat tcgatccgtg cgggccgccc
    10921 gcgcccgtcc cgggcgctct cgttatcgcc cgaccgtcgc cgtcgaaggc gtgacctcgc
    10981 tggtctgctt ggtcccgagc agatggatca ggccgacggc gaagttggcg gcggtcacac
    11041 cgcccagtcc gcgcatcgtc gagttcgact tgcccacccg ccggtccacg tccgccgtct
    11101 gatccgccac gctggtgagg cgccggttca gcgacgcatt ctgctcggcg agcgcgaggg
    11161 aatcggccac ccgctgcgcc tggaacgcga cgacgcgatt gttgctcgcg accaggtcgg
    11221 cgcgaagagc atcgttctcc gcgctcaggc gcgcgctctg ctgctgcagg gctccgagcg
    11281 tcgccgtcag ctcctcctgc tccttggaga gctgcgccag gttcgcggac atgcgcgcga
    11341 tcttgacgcc ggcctgcgcc aggctgctct cggcgaccgc cgccgcgatc cccgcgatcc
    11401 ggctgtcggt ggtcttctcg ttcacgcgct gcatcagatc ctggatgtcc gcgacgaact
    11461 tgcctgacgt gccggccgcc acggcacggt tccgctgctc gcgagcctcg ttcacccgga
    11521 ttcccgccac gatcagcccg gagaggacga gcaccgcgac gaccagtcct cccatgaccc
    11581 agttgcgaat cgtccactgc ttctcgtgaa tgtcgatgac cggagattcc ggagcttcat
    11641 ccgatagccg tctcatctcc cgcctcctgg gcgcaccggc ccgaaacgaa aaaacgccct
    11701 ctcgcggcac cttcgccgcg cggggcgcta tcgagttggc ctgccaatgg cccctccttt
    11761 cggggacctg cttccggcgt tacgaaccta cggtacaccc gtgggccggt accgtttcgt
    11821 actacctaag taccatccct tgtcggcgct cctcgatgcc ccttagactc gggacgcccc
    11881 accgatccca caacggagga ccctgtgaac gcgctcgaat ccttgaaggc gcgcctgctc
    11941 gaagcgagcg acctcgagta cgcggccagc ctgctgcact gggaccagac gacctacatg
    12001 cctcccggcg gcgcggaagc gcgcggccgg cagatcgcgc tcctctccaa gctcgcgcac
    12061 gagcggttca ccgccccgga gacggggaag ctgctcgagg cggcggagcg ggagacggcg
    12121 tcactcccgt acgactcgga cgaagcctcg ctggcccgcg ccacccgccg cgactgggat
    12181 ctccagatcc ggcggccctc gagcctcgtg gcggagctgt acgagcaggc ggcgcggagc
    12241 taccaggcgt ggatcgcggc gcgcccggcg aacgatttcg cgagcgtccg gccgcacctg
    12301 gaacgcacgc tcgaactctc gcggcgcatg gccgactgct atcccggcta cgaccacatc
    12361 gccgaccccc tgatcgcgtt cgccgactac ggcatgaagg cggagtcgat ccgcacactg
    12421 ttcgccgagc tgcgccgccg cctggtgcct ctggcgcagg cgatcacgtc gcgcccgctc
    12481 gccgacaatt cctgcctcca ccggcgttcc cggctggagg accagacggc gttcggcatc
    12541 gacgtgatcc ggcgcttcgg gtacgacttc cagcgcgggc ggcaggactt cacgcgccat
    12601 cccttcatga ccaagttctc gctcggcgac gtgcggatca cgacccgcgt gaacgaggac
    12661 gatctgacgg acatgctctt ctccacgctg cacgaatgcg gtcatgcgct ctacgagcag
    12721 gggatcgacc ccggctacga ggggaccccg ctcgcgtccg gcacctcctc cggcgtgcac
    12781 gagagccagt cgcggctgtg ggagaacctc gtcgggcgca gccgcggctt ctgggagttc
    12841 ttcttccccc gcatgcaggc cgcttttccc gaacagctcc gcggggtctc gctggacgcg
    12901 ttctacaagg ccgtgaaccg cgtcgagcgc acgctcatcc gcaccgattc cgacgaggtc
    12961 acctacaacc tgcacgtgat gctccggttc cagctggagc tggatcttct ggaagggaaa
    13021 ttggccgtcg cggatctccc gcgcgcctgg cacgagcggt tcgaagcgga catggggctg
    13081 cacgttcccg acgatcggaa cggcgtgctg caggacgtgc attggttcag cggccgcatc
    13141 gggggcgtct tccagggcta cacgctcgga aacatcctct cggcgcaatt cttcgacacg
    13201 gcggtgaagg ctcatccggc catcccccag gaaatccaga acggagaatt cgggacgctg
    13261 cacggatggc ttcgcgagaa catctaccgc cacggcgcca agttcacccc cgccgagctg
    13321 gtgcggcgag tgacgggacg ggacctcacg gtcgagccct acatggacta cctctggcgg
    13381 aagttcggac cgctctacgg gctccggaaa gaggaactgg gcggcgtgag cgtgggatga
    13441 gtccccggcg caagcgcgag tggttcgacg acgacgggtt ctggcgcgat ctgtatccgt
    13501 tcatgtttcc cgagacgcgg ttcgaggccg ccgtcctgga tacgcccaag ctgattcggc
    13561 tcgcccggcc gcgcggaagg tcggttctcg acctggcctg cggccccggc cgcttctcga
    13621 tcgcgttcgc gaagcgcaag taccgcgtca ccggcgtcga ccggacgcgc tatctgctcg
    13681 accgggcacg ggcgagggcc aggaccgcca aggtccaggt ggaatggatc cgccaggaca
    13741 tgcgcgactt cgtgcgcccc gacgccttcg atctcgcact cagcctgttc acatcgttcg
    13801 gatacttcca ggacaagagc gaggacgtgc gggtgcttcg ccaggtcctc gagagcctga
    13861 ggcccggcgg cgccctcgtg gtggaagtca tggggaagga gcagatcgcc ggaatcttcg
    13921 cccccgtgag cgcagacaag ctcggggacg cgctcctggt ccagcgccgc gagatcttcg
    13981 acgactggac gagaatctgg aacgagtgga ccctcatccg aggcggccgc gccaagacgt
    14041 acgagttcac ccataccatc tactccggtc aggagctgcg cgagcgcttc gagcaggccg
    14101 gctattccga cgtgaagttg tacgggaatc tcgagggaga accctaccag cggcgctcgc
    14161 cgcgcctggt cgccgtgggg tggaagggga gcaggcagta ggcaacggaa tcgaagtcgt
    14221 tcctgcggcg ttttgagtca gcggtactcg tgtcccaaac ccggcccagg aggaacacca
    14281 tgcgacgtgc tctgatcctc gccgctttcg ttctgatgag cttcgttccc gtccaggcgt
    14341 tcgccgcgtg gggtctcggt gccaacctgg gcatgaccct tcacaacccg gccgagaccg
    14401 gatcggacaa cgtcaccctg atcgggttcc ccagccaggc cagcgtgttc acgtcgctgc
    14461 gccccgggct gcgggtcggg tttgccgggg attcctcgcc gcacgaggtg tacctggacg
    14521 tgagcctgga tcacgaggcc tcgggtggag acagtttcac cgcggttcgc ctcggcggga
    14581 actaccagta caacttcagc ggcacttcct tccggccgta cctcacggcc ggtctgggcg
    14641 tcttcagcga gcacgccgat ttctcgtcga gcaccagcgc gacgtcgatg accttcggcg
    14701 gcggggcggg cgtcggattc ccgatcggcg accaagccgg acgcctccgc ctggaagcgc
    14761 gctacgacaa gctgcaggag gggaaggaca atggggacgt cgtgatcggt gaggcaggca
    14821 tcacgagcct gctcttcgga ttcgatctct ggctgcgatg acgagaccga acgtgagaac
    14881 caagccgccc atcgctccga tcctggttct cggactgttc gccggactcc tcgcggccct
    14941 gacctcgtgc ggcggcggcg gcgacgcgca gcccgcgctc actgcggccg aggccgacac
    15001 tctgatgaag tcctacgcca aggaccgctc cgacaccgag acctggctca agtcctcgcc
    15061 gacgtcctac ctggccacga cgcagcgcca ggacttcgga gatcgcacca cgatgacgat
    15121 cggaagcgcc gcggggagcg acgtacgcat cgaggacccg gcggtgaagc cgcgccatct
    15181 ccgcgtgacg gtcgagggcg actcgttcca cgtcgagggg gtggaccgcg gcgccacgtt
    15241 cgagatcctc aaggactcca cgacggcgac ctcggccacg gtgcccccgt ccagcatcaa
    15301 ggtcgcgcgc ttcacgcttc ggctctcgca ccagcgcttc ccggcgatca tcgtcttcga
    15361 tccgcagagc ccgcgctacc agctgtacaa gggggtgaag tatttccccc ccgatctcgc
    15421 gtatcacatc gtggccgtcc tcacgcccaa tccgcgtccg gacacgacga tcatcctctc
    15481 gactcgcggc aaccgccggc gcgccgtgcg cgtgggctgg ttcgacttca agctgaaggg
    15541 gacacccgtg cggttggagg cgaaccgcct gctcgagccc ggcgtcgggg agaaggacat
    15601 gagcctcttc ttcaccgacg cgacgaccgg gaaggagagc tacggcgtgg ggcgctatct
    15661 ggatcccgtg ccgcggcccg acgggcgcta cgtgctcgat ttcaacaact gctacaaccc
    15721 cgcctgcgcc tattcggagc actacaactg cccgatcccc ccggccgaga atcacctcaa
    15781 ggtcgcgatc gcggcggggg agatggattc gcactatatg cactaaccgg atccctgagc
    15841 cgcggcggcc ttggattccg cgttatactc gaagctcacc tcttgaaatc ccgagatccg
    15901 ggggcaccgg gtcgtgcttt gccgtttcgg aacggaggaa tcagccgtga gccgcaaccc
    15961 gttctcgtcg cgtttccgtc gattcctctt catcttcgtg ctcgttgcat ccgtggccat
    16021 cgcctcgccc gccagcgccc ggatcggcat cggcaacccg gtcaagaaga tgaaggagaa
    16081 gatccagaat aaggccgagg gcaaggccgc tccggaagcg acggaagctg cgggcccgcc
    16141 cacgttcgac gatgtcaccc tcgagctcac cgatgcccgg atcgccggca tcatcgacgc
    16201 gtaccagaag tcgaagaaga tcgccgccga tcgcgccccc ctcaacgcga agcgcaatca
    16261 gctgatggat gagcgcaaca agctgagcga caaggaaggg gacaagatgc gcgagcttcg
    16321 cgaaaagcgg ggcgacatgg aacagtgcta ccagagcggc tatcgggccg cgggggacaa
    16381 gaaagccgag gactacaaga atcgcgctct ctccgacccg gtgctccgcg acaagttcac
    16441 gaaggcggcg atgcaataca acgaggccgc cgccaagggc gacagcgccg ccatccagaa
    16501 gctgcagtcc atcctcaccg gcgagatcct gccgtccaag gaggactcgg ccgcggtgcg
    16561 cgccaagtgc gggccgatgc cgcccaagtc cgccgccgag atcaaggacg agcagctctc
    16621 caaggacatc gatgcggtcg acgaacagat ccgcaagatc gatgaggaag tctccaaggt
    16681 gggaaacgga ggcatgaacc cgcagcagtg gggcacggcg ctcgagcgca tccagctcta
    16741 cgtccagagc aagaaggcga agaaggagcc caagatgctc accgaccagg agatccgggc
    16801 cgtcgagaag cgtctcgccg agctcgaaga ggcgatgggg atctagccgt gcgcgcgccg
    16861 cgcgcgatgg ccgtcgcggc ggccttcgtc gtcgcgtgcg tggcttcgcc cctcgccgcc
    16921 gaggggcccg cgaccctcgc gttcgaccag cacggcacct ggcatacctt ctggcgctcc
    16981 gaccaggcgc ctgcccgctg gtcggggccc gactcgatgt tgagccgctc gctcgtctgg
    17041 aagcggctcg cgcgggggtc ggactgggcg agcgtccggc tcgcctgtgc cgcccccgtc
    17101 tggcgcgccc gcgtgatcgt ggcgcgactc gatccccggg aggtggcgct ctcgctcgcg
    17161 atggatctta cccgcgccga gggccggccc gcgtggagca tcgaccgcgc tcccgaagat
    17221 gccatcctcg ccgtgaacgc ggggcagttc atccgcacga tgccctgggg ctgggtcgcc
    17281 atggacgggc gtcaacgcct tcgccccggt ttcgggccgc tttccagcgc ggtggcggtc
    17341 gacgcctcgg ggcgcgtgcg ctgggcgcac ggcgactcgc tcctcgacgc gaccggggtg
    17401 gtcgcgggct tccagtccta cccgaccctc ctcgagggga acggggtcgt gcccgcgatg
    17461 ctccgcagcc tcaatcacct cgtgagcctc acccatcacg actcccggct ggccctgggc
    17521 cagacccgcg acggccggct cctggtcgtg atgacgcgct tcgacgcgat gggggaggag
    17581 gcgggcggat ttccgctcgg ccccacgacg cccgagatgg cggcgatcat gggcgccctg
    17641 ggggcgagcg acgccgtgat gctcgacgga gggatctcgg cccagctcat gctccgagac
    17701 gggaacggga agacgctccg ctggcccggc gtgcgcaagg tgccgctcgc gctgatcgcc
    17761 cggtcccggg tcgcggcgcc ggccgtgcgc gcctccaagt agtcccgccg ggggcgtcgt
    17821 atactcccga ccgtgatcgc ttttcattct tcccgccacg aagagcacgc gccgccgttc
    17881 gaataccatc gcggcgagat cgtgccctgc ttcgagacgc cagcccgcat ccggatcgtg
    17941 tgcgacgagc tcgcggcccg cggacaccgg ttgcgcgagc ccgatcagga cagcgcgtcc
    18001 cttctgcccc tggtgcatac gccgcgctat ctggcctttc tcgggacggc ctgggaccag
    18061 tggcttgaga ccgaccgcgg ccgggccgag gtccagccct tcccggcggt gtggccggtg
    18121 cgcacgctgc gcggcgacgt ggagcgg
//
LOCUS       DASSLZ010000129         4417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1436677, whole genome shotgun sequence.
ACCESSION   DASSLZ010000129 DASSLZ010000000
VERSION     DASSLZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436677"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1027
                     /gene="hemC"
                     /locus_tag="VFM00_04845"
     CDS             131..1027
                     /gene="hemC"
                     /locus_tag="VFM00_04845"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET7497607.1"
                     /translation="MSHTIKIGVRGNRLGMSIGKQTAEELRPHLGGATIEFVPIADPG
                     RRTTSPWPGGALEHALTQGEVQMVIQNAKDASPATSGVSIAAVTTRFTPFDVLIAPDD
                     AILDELPEGAALSAHTALRRAQLLNYRNDFRIVDFAGSLEERIKMLERGDVDGLVVSA
                     SAVEHLKLQDRVTEIFTTAVLIPAPGQGSAVIQVRSSDKDLVKAAKHLNDPDAHAEFD
                     AERAFLKALGADARAPVGALAQNDGASIRLEGVVAEKDGARLFRDTESGEPKEAAEIG
                     ERLARRLLAEGGRSALAAAESN"
     gene            1029..2300
                     /gene="serS"
                     /locus_tag="VFM00_04850"
     CDS             1029..2300
                     /gene="serS"
                     /locus_tag="VFM00_04850"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET7497608.1"
                     /translation="MLDAKRIRGDRDKVEEAIRYKKVPVDLPRYLERDERRRELLVRT
                     EALKHRKNEASEEIGKKKRSGEDAATAIAAMKGVSEEIKALDSELAEVDAELDRLSKW
                     IPNVPHESTPRAEADGNQVVRSWGEPKKFDFAPKPHWELATALGLLDFERAGKIAGAG
                     FLLFTGKGARLERALINFMIDLHARDHGYTEVWPPHLVRSDCLESTGQLPKLAEDMYR
                     LADEDLYLNPTAEVPVTNIYREEIVDGAKLPIRLVGYCASYRREAGSYGRDTRGMIRV
                     HQFDKVELVKIVRPETSYDEHESLCRDAEDVLRALGFAYRVVLLAAGDLSFAAAKCYD
                     LETWAPGEGRWLEVSSCSNFEDFQARRAGIRFRTETGKAELAHTLNASGLALPRTFAT
                     LLENGQTERGTVLLPEPLVPYMGGMRELTPD"
     gene            2325..3611
                     /locus_tag="VFM00_04855"
     CDS             2325..3611
                     /locus_tag="VFM00_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7497609.1"
                     /translation="MARASEAPRDYRPSYKVYLFLAMVVLSMALVAHSHFVIRRLNEQ
                     ARNLSAVLARFIAVSTLGAAEQPTLRPIFLEVIRNINFPMVLTDTKGVPRAWKGIGVD
                     PQAVPDSVLAAAESTGVIPPVVKRIQETAARLDRVNRPIPVVRLGTPGVLGHVHYGEP
                     ALAAQLRWIPYIEFGGIVLLLVLGFAGYRGLKAGEQRALWAALAKETAHQLGTPLSSL
                     MGWTSVLREAGQEGRIDPARAESIADEMDRDLDRLHRVANRFSQVGATPALAEGDLTR
                     AVAETVDYVRSRLPTLGRAVTITERYDPIPPVRFHRELFTWVVENLLKNALDAADKPD
                     ATIEVSLLWRRDERLVELRVRDNGRGMSVAERRRALETGFTTKRRGWGLGLALASRVV
                     REYHRGRISIVESAPGRGTTVAVALPVSLHRQAPRA"
     gene            3608..>4417
                     /locus_tag="VFM00_04860"
     CDS             3608..>4417
                     /locus_tag="VFM00_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HET7497610.1"
                     /translation="MSPSEKKILWADDEIDLLKPHILLLEEKGYKVTPVTNGADAVAC
                     TARERFDAVLLDENMPGLGGLETLEAIKEQHPGLPIVLVTKNEAESLMDDAIGRRIDD
                     FLLKPIRPTQIFLALKRILESDRLQDSRRARDYVAELARLRAESAGGLGWRGWVDLYA
                     RNAQWDVELDGVDDEGLKQAQADQRRELNVEFSQLVEAQYERWARGEDAPALSTDVVS
                     RWVAPALRQGRRVTLIVIDCMRLDQWLTVEPLLRPFFDIALDHHVSILPTAT"
BASE COUNT          702 a         1546 c         1549 g          620 t
ORIGIN      
        1 taacatatat gttaatagcc ctattgggaa ggagggttcc gcggcacccc tcgccggcac
       61 gggcggcctc gcggcctggt gctgcgccgg ccgtgcgcgt ctcgcccggt tgtgctagcc
      121 tcgcactccc atgagccaca cgatcaaaat cggcgtccgc ggcaatcgtc tcgggatgtc
      181 catcggcaag cagaccgccg aggagctgcg cccgcacctg ggcggagcca cgatcgagtt
      241 cgtgccgatc gccgacccgg gccggcgcac cacgtcgccc tggccgggcg gggccctgga
      301 gcacgcgctc acgcagggtg aggtgcagat ggtgatccag aacgccaagg acgcctcccc
      361 ggcgacctcg ggcgtttcga tcgccgccgt caccacgcgc ttcacgccgt tcgacgtgct
      421 catcgcgccc gacgacgcca tcctggacga gctgcccgaa ggggcggccc tctcggcgca
      481 caccgcgctc cgccgcgcgc agctcctcaa ttaccggaac gatttccgga tcgtggattt
      541 cgcgggcagc ctggaagagc ggatcaagat gctcgagcgc ggcgacgtgg acgggctcgt
      601 ggtctccgcc tcggcggtgg agcacctgaa gctccaagac cgggtcaccg agatcttcac
      661 caccgcggtg ctcatccccg cgcccgggca gggctccgcc gtgatccagg tccgctccag
      721 cgacaaggac ctggtcaagg cggccaagca cctgaacgat cccgacgcgc acgccgagtt
      781 cgacgccgag cgcgccttcc tgaaggcgct gggcgccgac gcgcgcgcgc cggtcggcgc
      841 gctggcgcag aacgacggcg cgtcgatccg gctggaaggc gtggtcgccg agaaggacgg
      901 ggcccgcctc ttccgcgata cggagtcggg cgagcccaag gaagcggcgg agatcggcga
      961 gcggctcgcg cgccggctcc tggccgaggg cggccgcagc gcgctcgccg ccgccgagag
     1021 caactgacat gctcgacgcc aagcgcatcc gcggcgatcg cgacaaggtc gaggaggcga
     1081 tccggtacaa gaaggtgccg gtggacctgc cgcggtacct cgagcgcgac gagcggcggc
     1141 gcgagctgtt ggtccggacc gaggccctga agcaccggaa gaacgaagcc tcggaggaga
     1201 tcggcaagaa gaagcgcagc ggcgaggacg ccgccaccgc gatcgccgcg atgaaagggg
     1261 tgagcgagga gatcaaggcg ctcgactccg agctcgcgga ggtggacgcg gagctggacc
     1321 ggctctccaa gtggatcccg aacgtcccgc acgaatccac gccccgcgcc gaggcggacg
     1381 gaaaccaggt cgtccgctcc tggggcgagc cgaagaaatt cgatttcgcg cccaagcccc
     1441 actgggagct ggccaccgcg ctcggtcttc tcgacttcga gcgcgccggc aagatcgcgg
     1501 gggccggctt cctgctcttc acggggaagg gagcccgcct ggagcgcgcg ctcatcaact
     1561 tcatgatcga cctgcacgcc cgagaccacg gctacaccga ggtctggccg ccgcacctgg
     1621 tccgctccga ctgcctggaa tcgacggggc agctcccgaa gctggccgag gacatgtacc
     1681 gcctcgccga cgaggatctg tacctgaacc ccacggccga ggtccccgtc accaacatct
     1741 accgtgaaga gatcgtggac ggggcgaagc ttccgatccg cctggtgggc tactgcgcgt
     1801 cgtaccgccg cgaggcgggc tcgtacgggc gcgacacccg cgggatgatc cgcgtgcacc
     1861 agttcgacaa ggtggaattg gtgaagatcg tccggccgga aaccagctac gacgagcacg
     1921 agtcgctctg ccgggacgcc gaggacgtgc tgcgggccct gggattcgcc tatcgcgtgg
     1981 tgctcctggc cgcgggcgat ctctcgttcg ccgccgccaa gtgctacgac ctcgagacct
     2041 gggcgcccgg cgagggacga tggctggaag tttcctcctg cagcaacttc gaggatttcc
     2101 aggcgcgccg cgcggggatc cgcttccgca ccgagacggg caaggcggag ctggcccaca
     2161 ccctgaacgc ctcgggcctc gctttgccgc gaaccttcgc cacgctactc gagaacggcc
     2221 agacggagcg cgggacggtc ctccttcccg agccgctggt accctacatg ggcggaatgc
     2281 gcgagctgac cccggactag acgggcgcgg cccccggcga cgaattggcg cgcgcgagcg
     2341 aagccccgcg ggattacagg ccctcctaca aggtctatct cttcctcgcc atggtcgtgc
     2401 tcagcatggc cctggtcgcc cattcccatt tcgtcatccg ccgcctgaac gagcaggcgc
     2461 ggaacctgag cgcggtgctc gcgcgcttca tcgcggtctc cacgctgggg gccgcggagc
     2521 agccgaccct gcgtccgatc ttcctcgagg tgatccggaa catcaatttt ccgatggtgc
     2581 tgaccgacac gaagggcgtg ccgcgcgcat ggaaggggat cggcgtcgat ccgcaagcgg
     2641 ttcccgattc ggtgctggcg gccgccgaat cgaccggcgt gatccctccg gtggtgaagc
     2701 ggatccagga gacggcggcg cgcctcgacc gcgtgaaccg gccgatcccc gtggtgcggc
     2761 tcgggacgcc cggcgtgctg ggccacgtgc actatggcga gcccgcgctc gccgcacagc
     2821 tccgctggat tccctacatc gagttcggcg gcatcgtgct cctgctcgtc ctgggattcg
     2881 cgggataccg cggcctcaag gcgggggagc agcgcgcgct gtgggccgcg ctggccaagg
     2941 agaccgcgca ccagctgggc acgccgctct cctcgctcat gggatggacc tccgttttgc
     3001 gcgaggcggg acaggagggg cggatcgacc ccgcgcgggc ggaatcgatc gccgacgaga
     3061 tggaccgcga cctcgaccgc ctgcatcggg tggcgaaccg cttctcccag gtcggggcca
     3121 cgccggcgct cgccgaaggg gacctgacgc gcgccgtggc cgagaccgtg gactacgtcc
     3181 ggagccggct cccgacgctg ggccgcgcgg tcacgatcac ggagcgctac gatccgatcc
     3241 cgcccgtccg cttccaccgc gagctcttca cgtgggtcgt ggagaatctc ctgaagaacg
     3301 cgctcgacgc ggcggacaag cccgacgcga cgatcgaggt gtcgctgctg tggcgccgcg
     3361 acgagcggct ggtggagctg cgcgtgcgcg acaacggccg cggcatgagc gtggccgagc
     3421 ggcggcgcgc gctggagacg ggcttcacga cgaagcggcg cggctgggga ctgggcctcg
     3481 cgcttgcgag ccgcgtggtg cgcgagtacc atcggggacg gatctccatc gtggagagcg
     3541 cgcccggccg gggaaccacc gtcgccgtgg cgctcccggt ttccctccat cggcaagccc
     3601 cccgcgcatg agcccgtccg agaagaagat cctctgggcc gacgacgaga tcgacctgtt
     3661 gaagccccac atcctgctgc tggaggagaa ggggtacaag gtcacccccg tcaccaacgg
     3721 cgcggacgcc gtcgcgtgca ccgcgcggga gcgcttcgac gccgtcctcc tcgacgagaa
     3781 catgccgggg ctgggcggtc tcgagacgct cgaggcgatc aaggagcagc atccggggct
     3841 ccccatcgtt ctcgtcacga agaacgaggc ggagtcgttg atggacgacg ccatcggccg
     3901 ccgcatcgac gacttcctgc tcaagccgat ccgccccacg cagatcttcc tcgcgctgaa
     3961 gcgcatcctc gagtcggacc ggcttcagga ctcgcgccgc gcgcgcgact acgtggccga
     4021 gctggcgcgc ctgcgcgcgg agtcggcggg cggcctgggc tggcgcgggt gggtggacct
     4081 ctacgcgcgg aacgcgcaat gggacgtgga gctggacggg gtggacgacg aggggctgaa
     4141 gcaggcgcag gccgaccagc gccgcgagct gaacgtcgaa ttctcccagc tggtcgaggc
     4201 ccagtacgaa cgctgggcgc gcggcgagga cgcgccggcg ctctccaccg acgtggtctc
     4261 gcggtgggtg gctccggccc tgcgccaggg acggcgcgtc acgctgatcg tgatcgactg
     4321 catgcgcctc gaccagtggc tcacggtgga gccgctgctc cgccccttct tcgacatcgc
     4381 cctcgaccat cacgtctcga tccttcccac cgccacg
//
LOCUS       DASSLZ010000130         7844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_160056, whole genome shotgun sequence.
ACCESSION   DASSLZ010000130 DASSLZ010000000
VERSION     DASSLZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160056"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFM00_04865"
     CDS             <1..717
                     /locus_tag="VFM00_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET7497611.1"
                     /translation="HGGPHGIAGDNFHFRWNLHAFAAPGYVVAAVNFHGSTSWGQEFA
                     ACIQGGHGDKPYDDIMKATDTLIEAGFVDPSRMAATGGSYGGYLVCWIAGQTDRFRCV
                     VNHAGVFDLLAEYGSDVTQGRHQAYGGEPWDRLEAIDRWSPARFARGFSTPMLVIHGE
                     RDFRVPHNQAMAVYNIYKAKGVDARLVFFPDENHWILKPRNSLLWNREVRDWLARWLG
                     PTPAGPASGLPGSAGSAAGA"
     gene            741..1976
                     /locus_tag="VFM00_04870"
     CDS             741..1976
                     /locus_tag="VFM00_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7497612.1"
                     /translation="MRWSWQIARVAGIPIRIHATFLLLIGWIVFGVLAEGQGVAPAIE
                     AAILLLAVFACIVLHEFGHALTARRYGIRTRDITLLPIGGVARLERIPERPLHEFLVA
                     IAGPAVTLLLAGLLLGLLAAAWPVEARRIVAFLNPVWNVLSGNPPLEGPGGFVAELFL
                     VNVMILFFNLIPAFPMDGGRVLRAVLATRLDYVRATQIAASVGQVLAVLLAVIGINGH
                     NLLLPLVALFVFLAAGQESSMAQMRTAFAGVPVSRAMIRDFRTLRADEPLTRAVELLL
                     AGDQQDFPVLGAFEGSPPVGILTRRDLMGALANGPRDRAVGDVARRDCGTAHPREYLE
                     DVFQRIDRSGCTALLVMEGGRVVGMVTLENVGELAMVHAALGRNARGRGANDHGRAAS
                     DHGRGANDGRAGGIGTGPV"
     gene            complement(1973..3583)
                     /locus_tag="VFM00_04875"
     CDS             complement(1973..3583)
                     /locus_tag="VFM00_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET7497613.1"
                     /translation="MAQRTEPETSQRQFDRRTWGILGLSILVILAFTATIPSLYMGLV
                     RSGFLGDRLPPGGGWMLLVGLIGLTALFCLYMVHQQHSINRLRDRMLRDQMELEQSRG
                     RLAELTSLFQLGNSLHMELPLETILEITVRRVASTLHSHDVTLFLYDPDAKSLSAKAA
                     FGLTPREPEPDVKLGEGAVGWAARHRDPILMAARERDARFAEFFAAHPDVGSALFLPI
                     ALESKTLAVLQVCRAARAEAFRLEHRDIGQLFAENVASVLDRAVVMDRLRRATSAVAA
                     AALPGPEPSGGAGEFRDSFLGAAAAELKSPLTAIVAYSEVLDQNDRKMTPSMRAEFTG
                     RLRSEAQRMMALVDDVLDLVRLELGRYLLELRVTNVNDVARGAIEAVRAQADAKQIEL
                     ELSLDRRIQNQHLDPAKVRQSIANVLRNGIRFSPMRSRIRVATWLRDDGVVIEIKDSG
                     PPVAPESASSVFDLESAVNEECKRCKDGLGFGLHLAKRFVELHGGSVSAGTDAAGGGA
                     VFWLTLPAGEDLAHVMGGDPFAGELAKS"
     gene            3708..5315
                     /locus_tag="VFM00_04880"
     CDS             3708..5315
                     /locus_tag="VFM00_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET7497614.1"
                     /translation="MSVLRSHLKTSSEEFQQNRKHHEALAADLRARLAAVKRGGSEQA
                     VSLHKNRGKLLARERIERLLDSDTPFLELSALAGGGMYGDEVPSGGIVTGVGVVSGRE
                     CMIVANDATVKGGTYYPITIRKHIRAQEIALENRLPAIYLVDSGGVFLPLQAEVFPDK
                     EHFGRIFYNQAIMSAAGIPQLAAVLGMCTAGGAYVPAMSDENVIVRGTGTIYLGGPPL
                     VKAATGEEVTPDELGGAEVHTRQSGVTDHMAANEEEALHILRDAVERLGTVKRVDLDL
                     AEPEDPLYPAEEIYGVLPPDTRRPYDVRELLARLLDGSRLHEFKREYGTTLVCGFGRW
                     LGYPVGVVANNGVLFSESALKGAHFVELCAQRRIPLLFLQNITGFMVGKRYEAGGIAK
                     DGAKMVQAVATAEVPRITVLIGASHGAGNYAMCGRGYRPRFLFTWPNSRISVMGAEQA
                     ASVLVQVKRDQLAREGKTLSDADAAAIADPVLEKYEREGSPYYATAQLWDDGVIDPAE
                     TRAAVGLALSAALNAPVTRGLAPVYRM"
     gene            5317..6132
                     /locus_tag="VFM00_04885"
     CDS             5317..6132
                     /locus_tag="VFM00_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020411587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7497615.1"
                     /translation="MSATPADSPKVRLDVEAGIARLTLTRPEVRNAFDEGLIAEITAA
                     LGVLREEFRSDGGLAPRLLVLTGEGTAFCAGADMNWMRRSIRYTREENEEDAFRFAKM
                     MLALDELPIPTIARVNGACLGGGMGLIACCDVVVASEDADFGFTEVRLGIAPAVISAF
                     VLPKIGVAAARRWFLTGEIFKAETARAMGLVHEIAPAGGLDAAVERLIAAIVGNGPRA
                     VAEAKRLIRENTALSRDLAIENSVRAIAALRASPEGQEGLTAFLEKRRPAWKK"
     gene            6181..7650
                     /locus_tag="VFM00_04890"
     CDS             6181..7650
                     /locus_tag="VFM00_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET7497616.1"
                     /translation="MIRKVLVANRGEIALRVCRTLRAMEIPSVTVYSDADRTAPHADF
                     GDESVRLGPASPADSYLNATALLEAAHATGADAIHPGYGFLSQSAAFARACREAGVVF
                     IGPSPESMERLGDKVSSRRSAARCGVPVVPGVEGIEDAARARAEADRIGYPVLLKAAG
                     GGGGRGMRRVDAAGDLAAAFESARREAAASFGDDRLFLEKLVEPARHVEIQILGDGTD
                     AIALGERECSLQRRYQKVIEESPSPAVDGKRRAAMEEAAVRLAKDAGYASAGTVEFLL
                     GPDGSFYFLEVNTRLQVEHPVTEMRFGLDLVRAQIEIAAGGGLPRRGRPEGFAIEARL
                     NAEDPYHGFLPQTGKILLLSWGAGPGIRVDAGIAEGQSIHAHYDSLLAKVIAHGATRE
                     EARTRLIAALSRTALLGVVTNQAFLLDLLEDEAFRTGRTFTRTIESRLWPGPEAPPDE
                     AVLAASIALTAPSRAAGAGERDDADRWSPWRLLGSWGRT"
     gene            7647..>7844
                     /locus_tag="VFM00_04895"
     CDS             7647..>7844
                     /locus_tag="VFM00_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497617.1"
                     /translation="MSRAVALRWNGLPVEARLSQKGDHAVLETGERRVEAVVAREGAW
                     IGLTIGERTLRAATARDARGIW"
BASE COUNT         1106 a         2788 c         2812 g         1138 t
ORIGIN      
        1 cacggcgggc cgcacgggat cgccggcgac aatttccact tccgatggaa cctgcacgcc
       61 ttcgcggcgc cgggctacgt cgtcgcggcg gtgaacttcc acggctcgac gtcgtggggg
      121 caggagttcg cggcctgcat ccagggcgga cacggcgaca agccctacga cgacatcatg
      181 aaagcgaccg acaccctgat cgaggcggga ttcgtggacc cctcgaggat ggccgcgacg
      241 ggtggaagct acggaggcta tctcgtctgc tggatcgccg ggcagaccga ccggttccgc
      301 tgcgtcgtga accacgcggg cgtgttcgac ctgctggccg agtacggcag cgacgtgacg
      361 caggggcggc atcaggctta cggcggggaa ccgtgggacc ggctggaggc gatcgaccgc
      421 tggagcccgg cccgcttcgc gcgcgggttc agtacgccga tgctcgtgat ccacggcgag
      481 cgcgacttcc gcgtgcccca caatcaggcg atggcggtct acaacatcta taaggcgaag
      541 ggcgtggacg cgcgtctcgt cttcttcccc gacgagaacc actggatcct gaagccgcgg
      601 aattccctgc tctggaaccg tgaggtgcgc gactggctgg cgcgctggct gggtccgacg
      661 cccgccggtc cggcgtccgg cctgcccggc tccgcgggga gcgccgccgg agcctagcgc
      721 gctcgggcga acacgcggcc gtgcgctggt cctggcagat cgcgagggtc gcgggcatcc
      781 cgatccggat ccacgcgacg ttcctccttc tcatcgggtg gatcgtcttc ggcgtcctgg
      841 ccgaagggca gggtgtggcg ccggcgatcg aggccgcgat cctcctcctg gccgttttcg
      901 cgtgcatcgt gctgcacgag ttcggccacg ccctgaccgc ccgccgctac gggatccgca
      961 cccgcgacat cacgctgctt ccgatcggcg gagtcgcgcg gctcgagcgg attcccgagc
     1021 gccccctcca cgaattcctc gtggcgatcg ccgggccggc cgtgaccctc ctgctggcgg
     1081 gccttctcct cggcctgctg gccgcggcct ggccggtgga ggcgcggcgc atcgtcgcct
     1141 tcttgaaccc ggtctggaac gtgctctcgg gcaatccgcc cctcgagggc ccgggcggct
     1201 tcgtggccga gctcttcctc gtcaacgtca tgatcctctt cttcaacctg attccggcgt
     1261 tcccgatgga cggcggccgg gtgctgcggg cggtgctcgc cacccggctc gactatgtgc
     1321 gcgccacgca gatcgcggcg tcggtggggc aggtcctcgc cgtcctcctc gccgtgatcg
     1381 ggatcaacgg ccacaacctg ctcctgccgc tcgtggccct cttcgtcttc ctggccgcgg
     1441 ggcaggagtc ctcgatggcc cagatgcgga cggcgttcgc gggcgtgccg gtctcgcggg
     1501 cgatgatccg cgacttccga acgctccgcg ccgacgagcc gctcacccgc gccgtcgagc
     1561 tgctcctcgc cggcgaccag caggatttcc ctgtgctcgg tgccttcgag gggtcgcctc
     1621 cggtcgggat cctgacgcga cgcgacctga tgggggccct ggcgaacggt ccgcgcgatc
     1681 gggccgtggg ggacgtggcg cggcgggact gcggcaccgc gcacccgcgc gagtacctcg
     1741 aggacgtctt ccagcggatc gatcggagcg gctgcaccgc gctcctcgta atggagggtg
     1801 gacgcgtcgt ggggatggtg accctggaga acgtggggga gctcgcgatg gtgcacgccg
     1861 cgctcgggag gaacgcgcgg gggcgcggcg ccaacgatca cggccgcgcc gccagcgatc
     1921 acggccgcgg cgccaacgac ggccgcgccg gcgggatcgg aacgggcccg gtctagctct
     1981 tcgccagctc tccggcgaag ggatcgccgc ccatgacgtg ggcgaggtcc tcgccggcgg
     2041 gcagcgtcag ccagaacacg gcgccgcccc ccgcggcgtc cgttcccgcg ctcacgcttc
     2101 cgccgtgcag ctccacgaat cgcttggcca gatggagccc gaagcccagc ccgtccttgc
     2161 agcgcttgca ctcctcgttc accgcgctct ccagatcgaa caccgacgag gccgattccg
     2221 gcgccaccgg cggcccggag tccttgatct cgatgacgac gccgtcgtcg cgcagccagg
     2281 tcgcgacgcg gatccggctc ctcatcgggg agaagcggat gccgttccgg agcacgttcg
     2341 cgatcgactg ccgcaccttg gcggggtcga gatgctgatt ctggatcctg cggtccagcg
     2401 agagctccag ctcgatctgc ttcgcgtcgg cctgcgcccg gaccgcctcg atcgctcccc
     2461 gcgccacgtc gttcacgttg gtcacgcgca gctcgaggag ataacgcccc agctccagcc
     2521 gcaccaggtc cagcacgtcg tccaccagcg ccatcatccg ctgcgcctcg ctccggaggc
     2581 ggccggtgaa ctcggcccgc atcgagggag tcatcttccg gtcgttctga tccagcacct
     2641 cggagtaggc cacgatcgcc gtgagcgggg acttgagctc ggccgccgcg gcgcccagga
     2701 agctgtcgcg gaactcgccg gccccgcccg agggttccgg ccccggcagg gcggccgccg
     2761 ccaccgcgga ggtcgcgcgg cgcagccggt ccatcaccac cgcgcggtcg agcaccgacg
     2821 ccacgttctc cgcgaagagc tggccgatgt cccggtgctc caggcggaac gcctccgcgc
     2881 gcgcggcgcg gcagacctgg agcacggcga gcgtcttcga ctccagcgcg atcggcagga
     2941 agagcgccga gcccacgtcg ggatgcgcgg cgaagaactc ggcgaagcgc gcgtcccgct
     3001 cgcgggccgc catcaagatg ggatcgcggt ggcgggcggc ccacccgacc gcgccctcgc
     3061 ccagcttcac gtccggctcc ggctcgcgcg gcgtcaggcc gaacgcggcc ttggccgaga
     3121 gcgacttcgc gtcgggatcg tagaggaaga gcgtcacgtc gtgcgaatgg agggtcgagg
     3181 cgacccggcg cacagtgatc tcgaggatcg tttcgagcgg cagctccatg tggaggctgt
     3241 tgcccagctg gaagagcgac gtcagctcgg ccaggcggcc ccgcgactgc tccagctcca
     3301 tctgatcgcg cagcatccgg tcccggagcc ggttgatcga gtgctgctgg tggaccatgt
     3361 agagacagaa gagcgccgtc agcccgatca gcccgaccag gagcatccac ccgcccccgg
     3421 gcggcaggcg gtcgccgagg aacccggagc gcacgagccc catatagagc gagggaatgg
     3481 tcgcggtgaa cgccaggatg accaggatcg aaaggcccag gatgccccag gtcctccggt
     3541 cgaactgacg ctgcgacgtt tccggttcgg ttcgttgtgc cacagctccc cccgatacgt
     3601 gacccgaccg gccaaccatc ggcattcccg gcccccttct cgaccccaat tcgggacggc
     3661 cgttccccct ttcccgaagc gggtttggcg gttacagtca cggctccatg agcgtcctcc
     3721 ggagccacct caagacgtcg agcgaggaat tccagcagaa tcggaagcac cacgaggccc
     3781 tggcggcgga cctgcgcgcg cggctcgcgg cggtgaagcg gggcggttcg gagcaggccg
     3841 tctccctcca caagaaccgc ggcaagctcc tggcgcgcga gcggatcgag cgcctgctgg
     3901 actccgacac cccgttcctg gagctgtcgg cgctggcggg gggcgggatg tacggcgacg
     3961 aggtaccttc gggcgggatc gtgaccggcg tcggcgtcgt ttccgggcgc gaatgcatga
     4021 tcgtcgccaa cgacgcgacg gtgaagggcg gcacgtacta tccgatcacg atccggaagc
     4081 acatccgcgc gcaggagatc gcgctggaga accggcttcc cgcgatctat ctcgtcgact
     4141 cgggcggcgt gttcctgccc ctccaggccg aggtcttccc cgacaaggag cacttcggcc
     4201 gcatcttcta caaccaggcg atcatgtccg cggcggggat tccgcagctc gcggccgtcc
     4261 tcgggatgtg caccgcgggc ggcgcctacg tgcccgcgat gtcggacgag aacgtcatcg
     4321 tgcggggtac cgggacgatc tacctgggcg ggccgccgct cgtgaaggcg gcgaccggcg
     4381 aggaggtgac ccccgacgag ttggggggcg cggaggtgca cacccggcag agcggggtga
     4441 ccgaccacat ggcggcgaac gaggaggagg cgctccacat cctccgcgac gccgtcgagc
     4501 ggctgggcac cgtgaagcgg gtggacctgg atctcgccga gccggaggat ccgctctatc
     4561 ccgcggagga gatctacggc gtccttccgc ccgacacgcg gcggccctac gacgtccgcg
     4621 agctcctggc gcggctcctc gatgggagcc gcctccacga gttcaagcgc gagtacggca
     4681 ccaccctggt gtgcggcttc ggccgatggc tcggatatcc cgtcggcgtc gtcgcgaaca
     4741 acggcgtgct cttctccgag agcgcgctca agggcgccca cttcgtggag ctgtgcgcgc
     4801 agcggcggat cccgctcctc ttcctgcaga acatcaccgg cttcatggtg gggaagcgct
     4861 acgaggcggg ggggatcgcc aaggacggcg ccaagatggt gcaggcggtg gcgacggccg
     4921 aggtgccgcg catcacggtg ctgatcggcg cctcgcacgg ggccggcaac tacgccatgt
     4981 gcggccgagg ctaccggccg cgcttcctct tcacgtggcc caattcgcgg atttccgtca
     5041 tgggggccga gcaggcggcc tcggtgctgg tccaggtgaa gcgcgaccaa ctggcgcgcg
     5101 aggggaagac gctgagcgac gccgacgcgg ccgccatcgc cgatccggtg ctcgagaagt
     5161 acgagcgcga gggaagcccc tactacgcga cggctcagct gtgggacgac ggcgtgatcg
     5221 atccggccga gacgcgcgcg gccgtggggc ttgcgctctc ggcggccttg aacgcgccgg
     5281 tcacccgcgg gctcgcgccc gtctaccgga tgtagcgtga gcgccacgcc cgccgattcg
     5341 cccaaggtac ggctcgacgt cgaggccggg atcgcgcgcc tcacgctcac gcgccccgag
     5401 gtgcggaacg cgttcgacga ggggctgatc gcggagatca ccgccgcgct cggggtgctc
     5461 cgcgaggaat tccgaagcga cggcggctta gcaccccgcc tgctggtgct gacgggcgag
     5521 gggaccgctt tctgcgccgg cgccgacatg aactggatgc gccggagcat ccgctacacg
     5581 cgcgaggaga acgaagagga tgcgttccgc ttcgcgaaga tgatgctcgc cttggacgag
     5641 cttccgatcc ccacgatcgc ccgcgtgaac ggcgcatgcc tcggcggcgg catggggctc
     5701 atcgcctgct gcgacgtcgt ggtggcatcg gaggacgcgg acttcggctt caccgaggtc
     5761 cggctcggca tcgctccggc cgtcatctcc gccttcgtac tccccaagat cggcgtcgcc
     5821 gccgcgcggc ggtggtttct cacgggggag atcttcaagg cggaaaccgc gcgagcgatg
     5881 ggactggtgc acgagatcgc gccggccggc ggactggacg cggcggtcga gcggctgatc
     5941 gcggcgatcg tggggaacgg accgcgcgcg gtcgccgagg cgaagcggct catccgcgag
     6001 aacaccgcgc tctcgcgcga tctcgccatc gagaactcgg tgcgcgcgat cgccgcgctg
     6061 cgcgcctcgc cggaggggca ggaggggctt accgccttcc tcgagaagcg ccgtcccgcc
     6121 tggaagaagt gacgcgtcgg tcccgcgcgg ccgcccaccc gctccgaacg ggagtgcgcc
     6181 gtgatccgta aggtcctggt cgccaaccgc ggcgagatcg cgctccgtgt ctgccgcacc
     6241 ctccgcgcca tggagattcc gtcggtcacg gtctattccg acgccgaccg cacggcgccc
     6301 cacgccgact tcggggacga atcggtgcgc ctgggcccgg cgtccccggc cgactcctac
     6361 ctgaacgcca cggcgctcct cgaggcggcg cacgcgaccg gcgccgacgc catccacccc
     6421 ggctacggct ttctctcgca gagcgcggcg ttcgcgcgcg cgtgccgcga ggccggcgtg
     6481 gtcttcatcg ggccctctcc ggagagcatg gagcgcctcg gcgacaaggt ctcgtcgcgc
     6541 cgctccgccg cccgctgcgg cgtgccggtg gtgcccggcg tggaggggat cgaggacgct
     6601 gcgcgcgccc gcgcggaagc ggatcgcatc ggctacccgg tgctgctcaa ggccgcgggc
     6661 ggcggcggcg ggcgcggcat gcgccgcgtg gatgccgccg gcgatctggc ggcggcgttc
     6721 gagagcgccc ggcgcgaggc ggcagcctcg ttcggagacg accggctctt cctggagaag
     6781 ctggtcgagc ccgcgcgcca cgtcgagatc cagatcctgg gggacggcac ggacgcgatc
     6841 gcgctcggcg agcgcgagtg cagcctccag cgccgttacc agaaagtgat cgaggagtcc
     6901 ccctcccccg cggtggacgg gaagcggcgc gccgcgatgg aggaggccgc cgtccgcctg
     6961 gcgaaggacg ccggttacgc cagcgccggg acggtggagt tcctgctcgg tcccgacggc
     7021 tcgttctact tcctcgaggt gaacacgcgg ctccaggtgg agcatccggt caccgagatg
     7081 cgcttcgggc tggacctggt gcgcgcgcag atcgagatcg cggcgggcgg cgggctgccc
     7141 cgtcgcggcc gtcccgaggg attcgccatc gaagcacggc tgaacgcgga ggatccctat
     7201 cacggcttcc tgccccagac cgggaagatc ctcctcctgt cgtggggcgc cgggccgggg
     7261 atccgcgtgg acgccggaat cgccgagggt cagtcgatcc acgcgcacta cgattccctc
     7321 ctcgccaagg tgatcgcgca cggggcgacg cgcgaggagg cccggacgcg cctgatcgcg
     7381 gcgctctccc gcacggccct cctcggcgtc gtgaccaacc aggcattcct gctcgatctc
     7441 ctcgaggacg aggcctttcg caccggccgg accttcacgc gcaccatcga gtcgcgcctc
     7501 tggcccgggc ccgaggcgcc gccggacgag gcggtcctcg ccgcctcgat cgcgctcacg
     7561 gcgccctcgc gcgcggcggg cgccggggag cgggacgacg cggaccggtg gtccccctgg
     7621 cgcctcctcg gctcatgggg ccggacgtga gccgcgccgt ggcgctccgc tggaacgggc
     7681 tcccggtgga ggcgcgcctt tcccaaaagg gtgaccacgc ggtgctcgag accggggagc
     7741 gccgcgtgga agcggtcgtc gcgcgcgaag gggcctggat cgggcttacg atcggcgagc
     7801 gcaccctccg cgccgccacg gcgcgcgatg cgcgcgggat ctgg
//
LOCUS       DASSLZ010000131        24041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1437682, whole genome shotgun sequence.
ACCESSION   DASSLZ010000131 DASSLZ010000000
VERSION     DASSLZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 24041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24041
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437682"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFM00_04900"
     CDS             <1..826
                     /locus_tag="VFM00_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497618.1"
                     /translation="AERQEQSAPTFAVLASFEMKSVHQSGKFTIDLNKYTTDNLTLRF
                     DENIGDMRSLMKEGDNFRQVNLDDPLYKQRELVVFVDGMNAKDFGDYINFATVQLRKK
                     HQGGDETQDEVRIDRKNFNSEGNAFKLLYGWKNDTDRRRWMNYEYQTTWSFFGGKEMQ
                     TPWKAANAGAINLAPPFQKHSVDLQADPDAITQAGVRSITVKIFYNLGDTEQVKQVTL
                     NASKGQLSDKIDFILPSDKANYDYQIDWRLKGNKTVSSGRKSASDAILFVDEVPAG"
     gene            837..2318
                     /locus_tag="VFM00_04905"
     CDS             837..2318
                     /locus_tag="VFM00_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497619.1"
                     /translation="MRRTTIVRIAAPLFTAALLLSASAASAQTDRDYLKNKLSFRYVG
                     TPNNLSTAVEVLDNAAVASGTENGTFWASPALSGLQGMVRKLLKAPGQGGDANLQYVV
                     AKTVKIQDRPVMILMVNDVTAPLSADAMNHWDACDDGAGHAWPCASNASTTDDQRQQC
                     AESRHQPVPARLDATWAGQMTLGQAAFNGGTAGSATGTFVHELVHTQDRSDRRAHMFT
                     ISRRSYHYGSDGTHYDIEAVPNLASTYQEGIANAVMMTVDTREAQRLFDWFANNDVMM
                     VEKNVPVPPGTGAGAAPCWTVVTTPSADIWLYNQLTAAHVREVQRTPNPYPNYAYFRI
                     RDLPPRFIVHNENIIALVFSEYARHLGLQKFMDALKTNDRTIFRVSTSPIAQLYNTLC
                     RAGLEGRPLSAVTGVNEAGPKPYLIPLAYADYFTAYRSQSKDDYKTIFENMLPQEWID
                     LYWDGYKDAVRSAVPIDATHTPQFTNLTDISIALGVNQSTAEH"
     gene            2336..3826
                     /locus_tag="VFM00_04910"
     CDS             2336..3826
                     /locus_tag="VFM00_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497620.1"
                     /translation="MIVLPRGSCPLLAAALLLAIAMALPAPASAGDADRTYLRSKLVF
                     YYVDQPNRTDLPPEILNNTAISNGTETGGFWTSTKVRGLQGLVRSLLQAPGHGGDDYM
                     QMVTAWIIKVLDKPVMMTLVNDVDAPLSSAAMDRWDACDDGNGHAWPCASNMATSDDY
                     RARCAKQNGTSAPARRDFWAGQMTLGQAVFNGSTAGKATGTFIHELVHTQDRSDRQDV
                     RFWLSHTAYSYGADGTHYSVEAVPNLRASYQEGIANAMRLAVDMDRRKEMFTWFANND
                     VVLVEKALPTRSVYLNDHPCASMWEFPSQDIWLYDQLKAAGAHEVTSTTPIPGYAQYQ
                     IRSIPPRFIVHNEYITSLTMAEYAWHLGLGKVLRAIKTNDAALFRTANSPIAKLYETL
                     CTMGLEGRPLASVQNVNEAGPKPYLIPLAYADFFTGYQSATKADYASIFENKLPQAWV
                     DMYWDGYKDAVRSAAVIDASHKPRFDQLTDIAIALGVNQSVADEAP"
     gene            3859..7887
                     /locus_tag="VFM00_04915"
     CDS             3859..7887
                     /locus_tag="VFM00_04915"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HET7497621.1"
                     /translation="MKAVPTCRALAALLLLSLAVSGGGFRSARAEDASAAPAAAGDAS
                     SASPVSATGLLPIFGVAVDFDALPNNAKPDDAVASVQKLWDARLKEAGFNVIQFPVDA
                     RELGDKGAARLAKLCAWAKTNNVRLAPALLGASPGEALPSDYPDKVGAFATKTIELVG
                     KGDPAAYAQIMFYQLERPLNHPGNHGPMDAAQAASLIANAVTKLHAAEQAALAGSSLQ
                     ATPVLAGVSFDYELIKVGAIANVPITDESYQQAYASSHDYLAAVLGAAPVDVVGLEWF
                     PGSLSSDGVDRFPDLIGKLQGDFPGKLLIVSTGYSTAAKGEADQSRYYTQTFNNLSDL
                     RTNQGVESSFAGILWRTAMDKPGGEPAPPSSKTLDEMASWNWQDRAAELTRMWNEPGS
                     DSKEMRWWVGRVESRFGLVGSSKDASKPGMPKAACALLAHLKTSLAAAAMSTGAGQIA
                     EELTAAASGPGGTKGLVGAVKDKLQAALFGMLDVWVAKTAESLVSGGGGGGDGGGGGG
                     GGGGGAPPPPPPPAPTNTADIYIAGASVDSEIKAGSPVSISVTVGNGGPATATGVSLY
                     LKQNNGTSDLTQSTPTSITPGSSQVVTLNWTPDHPGNYPHVTVEAYCDNDSQLANNKT
                     DPVDMDAAPGGGGGGGGGGGGGGGAGGLGGGMGGKLGGKYGGVMQVDPKVLGGVKGNL
                     VTKTDPGFIHIEAVRGISPMPMMNTMSAPGAGGGTPMAMRMGTGTGGGTAKAGGGVAM
                     IGGAGAATGGTGGAGGGTGGGTQTMAYRMEAAPSGPQPVALAFTVTNPFDHTFTSVKG
                     TLKIQDKVIATKDLGVLFPRQRRTVSFAQWTPPKTGTFPVRIDLAGLGPGGKPLSASA
                     MDQITVNPGGGAAPPTGTATRMVIGGAGARGGTSGGAVPVSMTRGPGTLTQTRLLTPL
                     IMAPAAPAIAGPATRSLYLAPGGTRPIGGMASILGLTPNSVLLAPFPVQAGNDVSITI
                     RLFNTERTAAKKVKVEAYVDGDKLGETTLDVPVARAVIASGFPTWKAKPGRHDVRAVV
                     TAGDRSGAASKPVDVRPPNGVGAGGVAMLMGRGAFTLSTLQMTTADVRLNPLAPAVGN
                     AVDVSVRVQNAGAADVKGVRVELFADGTRLGEVSGNIAAGKDFVFSGFPKWTAASGAH
                     KLLLRASAGGRIVEATRDVAVGAAIMLAKPLLTTATLVTPTTPAAPPSTQTPVVGLRT
                     TAVTGMTLMMVANPDLQVAPGDITFLPISPKAGDPLTINITVRNVGTGAANNATVRAI
                     LQADGAEVTRREFPVNLAASGMTSLSWPVTTPSGKVLTAVVTAIVANDARADNNQAQA
                     TTSVAIALQRQLSPMILQVQPK"
     gene            7904..10891
                     /locus_tag="VFM00_04920"
     CDS             7904..10891
                     /locus_tag="VFM00_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497622.1"
                     /translation="MSRVARAGNVSAWLSGLVLVASLAPPSLAQGVLSGFMNPIGLAS
                     SYPADWQRTNSFASMQLFNNAQEPLTADIKITLVRNGTEVATTPPVQRLYPVGGFAVP
                     TPDLTRWQSMQFAGKVGDAVDLTGRLPDGVYTVCVDISNVRGTATGTLYNAGSEVSQC
                     SQFISFFPKPPGLIAPQSGSVVSTPTPVFQWTPVLSNLGAQLPHLFRLVEVYPGQTPE
                     RAIEANRSLYEQVFGLNMPGGDLSSFAYPASAPYLLSGHRYAWRVQAFLTVGTSGNGG
                     GISTGLFAKRAVITQYNARALGENEGRSKVYTFTWNSDPLAAARAPAAQDSPASEDAR
                     MGNVWSPSGNGASAREADGLGDDALDADSDARQSASGEAGQSEGNNFADRLARMMASL
                     WKQGPGSAVSVARAGGKGALTPSGAPERVSMTNGASARYAPGDGRHRPTSDPSASAQG
                     QPAATETAQATDAGPVDSTAQAVVTPPPAPAAPPSSESAGLGPGWARLHGTASVSGET
                     YSSDGLSIPNRPDRSSRVVSGLSVGVIQDRMRIPVSALFSDDQVAFRQNINQVAVAPR
                     FRWAGLTAGNFSPQFGSYTLADATILGGGLELAPKNWHLGFVSGRARKSITQVSSALG
                     IIPQLERNMTAGRIGYGNPLTNSVEFSILRATDDPKSIAGTDTLSTPEGNTVYSVRAQ
                     GLMPTRHLRAIVETALSRYDRDRRADASSVDGQALGLQLFHETAMTRIGVKTEYLNGG
                     FMTLGNSGITGDRVDVGVDAHVAFLQGKLNLDGMAGLRNDAVSEALAAETRRRNYGLT
                     GSWQPGPRFGADAQMSIYSSESDASDSLFLGNSNTTRVYSVAPRTMWTLGKVQNSVTC
                     SATIQKSENGGPIADTKSLSLLGNYAAQVGSPLAITFSGSYTRTDFEIAVSELSTFGP
                     GFTLNSFGSKLFTNAQLQVTKTRTGNAGTDTDLTPRVEMRWNFAQRQSLILRGNFRRY
                     QYAIPGTPEFNERIASLEYVTNL"
     gene            11000..12148
                     /locus_tag="VFM00_04925"
     CDS             11000..12148
                     /locus_tag="VFM00_04925"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET7497623.1"
                     /translation="MPDPIPFHRPSITDAEVDAVTATLRSGWLTTGPRVRDLEQMVAD
                     YVGVHHAVALSSCTAALHLALIAAGVGPGDEVVTSPYTFVSTGESILYLGAKPVFVDV
                     ERATKNLNPKLVASAITLKTRAIVSVSIAGHPCHAQEIAAEAKRHAIPVIEDAAHSLT
                     ARIGDQPVGRQADMTCFSFYATKGVTAGEGGMVVTERAEWADRVRRLSLHGLSAAAWS
                     RYDKGGWWDYDVVELGYKYNLTDVQAALALAQMRRADAMRDRREAIAGRYTEALAGVR
                     GLATPSVLPGVRHAWHLYQIEIAEGGRTDRNALALALREDGIGTSVHFKPLHCFPWYQ
                     ERLGVRPGQFPAAESCYAGTLSLPIWPDMTDAQVDRVAERIRKHMSAA"
     gene            complement(12369..13469)
                     /gene="wecB"
                     /locus_tag="VFM00_04930"
     CDS             complement(12369..13469)
                     /gene="wecB"
                     /locus_tag="VFM00_04930"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HET7497624.1"
                     /translation="MKTILSVFGTRPEAIKMAPVLKALARESGIRSVVCVTAQHRQML
                     DQVLGLFGIVPDHDLDLMREGQTLTEITVGALTRLESYLAETNPDFLLVQGDTTTTMA
                     ASLAAFYRKIPVGHVEAGLRTHDNLYPYPEEVNRRVTSVIAARHFAPTRRARENLLRE
                     GIAEGQVVVTGNTVIDALLEVVAMQPPRAPALPLRGKRIVLLTAHRRENFGAPMQEIF
                     AAVHDLVKRYADVDVVYPVHPNPNVAGPAREALGSEAGVHLIEPLEYKAFSDLMAASH
                     LILTDSGGIQEEAPSLKKPVLVLRDETERPEAVEAGVVKLVGPHREKIVTEASRLLDD
                     PKAYAAMATGANPYGDGKAAERIVAMLRQALR"
     gene            13584..15863
                     /locus_tag="VFM00_04935"
     CDS             13584..15863
                     /locus_tag="VFM00_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7497625.1"
                     /translation="MTSVGEKLQAALGERYRVTRELNRGGMGTVFEAEDTKHRRAVAI
                     KVLNPELSEVIGPRRFQAEIEMAARLMHPHIVPLHDSGSGNGLLYYVMPLVTGESLRQ
                     RLTREKQLPVEDALRIAREVADALQYAHAHGLVHRDIKPENIVLSSGHALVLDFGVAQ
                     SSAPGAEAVTRTLAVLGTPLYMSPEQSAGGAVDARSDQYSLACVLYEMLAGQPPFTGG
                     TADSIGLQHRTLEPRAVTTLRASVPPPIALALARALSKAPADRFPTAAAFGESLAVLA
                     TPSLVPTPGGPRRDATTPGRPPGGRVMLAVLPLENMSADPEQEFFTDGMTEELIAHLG
                     RLQPKRLGVIARTSAMRYKKTTKSVEEIGHELGVDHVLEGSVRRAGERLRITVQLIQV
                     ADQTHLWAENYDRRMADVFDIQSEVAASVAKALEVQLLPSGRAAGSAGAAANPAAYEA
                     YLRGRFHWNRRTKEGFRLAIEAFEQALAADPDYARAHAGLADVYSVLPNWDSITFAET
                     APKAEAHAKRALELDPDLAEAHASYGAVLTNFYRDYVEAEREDRRAIELDPSYGLAYT
                     WCATVLMAQGRIDDAIAMNERAHRLDPQSLVVLINFGAMYFWKRDFERAAAYTLRALE
                     MEPSLTRHAAFVGIYRGLQGRVEEGIAYLSRLSPEQRQDPHVMAAIGFLNAKAGRPEP
                     AREALRQLDERAKSEAVPYVPIHFLLVALDERARLYASLEKRVGEGTIPPVTLRYAPT
                     FDPFREDPEFQALMARTGA"
     gene            complement(15867..17597)
                     /locus_tag="VFM00_04940"
     CDS             complement(15867..17597)
                     /locus_tag="VFM00_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7497626.1"
                     /translation="MNPKPVAAPVPAADLAPEKKARILVVDDEDQVVQIFRDLLTQQG
                     YDVVSCDNGDDAITTVTTGAFDLVLTDINLPGVDGLEVVRAAKAADKDTVVILITGYA
                     STTTAIDALRQGAYDYITKPFDLWETAKAIERGLESRNLILENRRLIVELERANAELQ
                     EHEEILRRKVERATYRLGTLYEAGKAMSLSLSLQATCDVVAAQAARITGARACLLFLH
                     DQGSDEFEAIAGIGVPPDRYQKLRYAMGVGFHGQVVQSAEPALVTGISEAVGVEEVLA
                     ALHAENALIVPLLSNESVIGTITAINLADGDEFTADDAEGLKMLASQAAISVTNAILY
                     ERTKELDRLKSEFVAVVSHEVRTPLTSINGSLELLGDERFHKLPAPQKELLGICQANT
                     QRLISLINDILDFSKLESSRLSLNFERMDVGRVLVEAAENIRSLAASRGLELEVKIDP
                     SAESVEADPMRIGQVATNLIGNAIKFSPDKGRIEVFASGDSSTVTISVKDYGRGIAQR
                     DLNRLFQRFAQLDSSTTRKAGGTGLGLVISKGIVEQHGGKIWVESALGRGSTFSFSLP
                     RQRPAEPLAP"
     gene            complement(17632..18888)
                     /locus_tag="VFM00_04945"
     CDS             complement(17632..18888)
                     /locus_tag="VFM00_04945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET7497627.1"
                     /translation="MKQRNGAGSKRLVEATERFIRSEQKLLEVLGGRRILKKNECEHF
                     AKDLALSAAKKRSSDVFASAIAINRKVMAVLGKGKGSEAKARVSDAEVMGELSAIAQR
                     SAAPADAFAAALGQVQRAVPFENATLFVLDRDNGTLVEAATVGDRIDLIGHVRFDRGA
                     GFSSWVAQSRKPVLLNDLHREGGSEAPTVRSFLSVPVLVQGDLVGVVNLSHSRPGAFD
                     EESASRIALMMLPVSAMVIRLILMREAERLGTTDEVTTLFNKRAFDRSLESEVGKAKR
                     YGHKLSVVVLDVNATGARERAGTVNTQVLSDMGKALKRAARGSDCVSRYGADEFRILL
                     PHTDTPEAMIAAERLRTLVEKQATARRRKITVSIGVATFPVDAQDSGSLNTRAERALL
                     AAKRGVAETAAIPAGTRGPLDEAAAH"
     gene            complement(18908..19294)
                     /locus_tag="VFM00_04950"
     CDS             complement(18908..19294)
                     /locus_tag="VFM00_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497628.1"
                     /translation="MGWVRTGSTAAKPSASRPVEIRIRFEPGLGAALYASLHEGATEP
                     LTVARILGRGKALLHFHGVTLMAHGAPEWKPGETFPVTVKELGPPMVLAPAGRGRDEG
                     GARVTIVDSAVVPGSARHQGDAPGAR"
     gene            complement(19300..20265)
                     /locus_tag="VFM00_04955"
     CDS             complement(19300..20265)
                     /locus_tag="VFM00_04955"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET7497629.1"
                     /translation="MAHLSGAPARVAAARSAARARARVHAEPAGISRRMALQRAAERT
                     VSLPTFPAVASRLIEEVARPEATSEEIGRILALDPALTARTLRLANSDFYGFPRKVGS
                     VELAVLVLGPSTIRDLVLTSSVVRALGRPGSSLEGLLAHSMAAGIAARALAERAKYRL
                     TGDAYAAGLLHDVGKVVLRESDPARFEKVLARCRTKGVASFEAERAHFGSDHAEVGGW
                     LAERWGLPSDIVEAIACHHRPETAARNVALASLVHIANFLAQRAGYPWASGIDAQGLD
                     ERAWEPVEPRAARREALAAELVSEVIRETEREKALFAEFRGSGEE"
     gene            complement(20265..21035)
                     /locus_tag="VFM00_04960"
     CDS             complement(20265..21035)
                     /locus_tag="VFM00_04960"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HET7497630.1"
                     /translation="MAFSSVLHALQNRSPRSRNRKERQKEIALSKYAPLVKYVVDRLA
                     LHLPKSVERDDLTSAAIIGLFDALEKFDASKGTKFETYAIWRIRGAILDELRSLDWAS
                     RSIRRKARTVEEAARELGQKLGRAATEEEVADALNLSPVELSRLMDEVHGTALLSLSK
                     AVSTEDDQDYIQLEDIVDDPAQADALEVIESEQAREVLLETIDGLPEQQRLVVALYYY
                     EEMTLKEIGEALHISESRVSQIHTRAVKTLKARLARVL"
     gene            complement(21051..21290)
                     /locus_tag="VFM00_04965"
     CDS             complement(21051..21290)
                     /locus_tag="VFM00_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497631.1"
                     /translation="MRALLVRLTAVTAIAAALLSGLVSAATHASWIVTILRSAIAFVL
                     VAAVGIGFGLILMRTALRRHYEEAHPASRRARGNR"
     gene            complement(21293..22309)
                     /locus_tag="VFM00_04970"
     CDS             complement(21293..22309)
                     /locus_tag="VFM00_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HET7497632.1"
                     /translation="MNPTSQPMIDERHERRALHECAHEGTRSAMTEIENKAESNVERR
                     KRPAKKARTPRAARKPAIVPPSNRPRRRIVAVTSGKGGVGKTNIVTNLAIALARQGIR
                     VLVVDGDLGLANVDLLLGIAPQYDLQDVILGTRSVADVVLEGPDGIRILPASSGVEEL
                     ANLDEYRTEVLIRSLAELERDVDLILIDCPSGIGRNAVSLAQVADPILVVTTPEPTAF
                     SDAYAMIKVLSRRSIRCVPALIVNEAASEEEAAAVARRVGSVAKRFLNLDIEYWGAIL
                     ADESVPKSVLRQEPFLSTYPYSPAATCVYQLARRVLGMAPKETAPEPGPFTVVTSDEV
                     PEGV"
     gene            complement(22482..22826)
                     /locus_tag="VFM00_04975"
     CDS             complement(22482..22826)
                     /locus_tag="VFM00_04975"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HET7497633.1"
                     /translation="MSMPWIYATAFAVSAAVLYGASRVLAARPKGSPDAEEVLPLHVV
                     SRTRVGIGRTLVLVEVDGRRLLLGSTKEHWAALADLGRAPGAATEEDVFEEIEDELNR
                     ASRAARQRRLRS"
     gene            complement(22823..23122)
                     /locus_tag="VFM00_04980"
     CDS             complement(22823..23122)
                     /locus_tag="VFM00_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor C-terminal
                     domain-containing protein"
                     /protein_id="HET7497634.1"
                     /translation="MSRLLTPEEIAALRGEAPYVPAPRERYHVVVDAGHADLTQEELS
                     ALRAGDVLPLTRAALSPVEVVANGTTVAYGELITLEGRAAVRVIAMAGTDRRSYR"
     gene            complement(23133..23285)
                     /locus_tag="VFM00_04985"
     CDS             complement(23133..23285)
                     /locus_tag="VFM00_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497635.1"
                     /translation="MPRASKSRSRKSHAAQVRGRAMSAVERLAMRRYLAFVRAVVGQT
                     TKSAAR"
     gene            complement(23660..>24041)
                     /locus_tag="VFM00_04990"
     CDS             complement(23660..>24041)
                     /locus_tag="VFM00_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET7497636.1"
                     /translation="TEDPAEHALDPEPYRVPPETAEALRAARRAGGRVVAVGTTAVRA
                     LESWARDGESSDGAWRETSLFVLPGFAFRVVDGMVTNFHLPKSSLLFLVSALAGRDRV
                     LRAYEEAVREGYRFYSYGDAMLIL"
BASE COUNT         3841 a         8422 c         8211 g         3567 t
ORIGIN      
        1 cgccgagcgc caggagcagt cggcgccgac gttcgcggtg ctcgcctcgt tcgagatgaa
       61 gagcgtgcac cagagcggga agttcacgat cgacctgaac aagtacacga cggacaacct
      121 gacgctgcgg ttcgacgaga acatcgggga catgcggtcg ttgatgaagg agggggacaa
      181 cttccgtcag gtgaacctgg acgatcccct gtacaagcag cgggagctgg tggtgttcgt
      241 ggacgggatg aacgcgaagg acttcggcga ttacatcaac ttcgcgacgg tgcagcttcg
      301 gaagaagcac cagggcggcg acgagacgca ggacgaggtt cggatcgacc ggaagaattt
      361 caacagcgag gggaacgcgt tcaagctcct gtacgggtgg aagaacgaca cggaccgccg
      421 gcgctggatg aactacgagt accagacgac gtggagcttc ttcgggggga aggagatgca
      481 gacgccgtgg aaggcggcga acgcgggggc gatcaacctg gcgccgccct tccagaagca
      541 cagcgtggat ctgcaggccg atccggacgc catcacgcag gcgggggtgc gctcgatcac
      601 ggtgaagatc ttctacaacc tgggcgacac cgagcaggtg aagcaggtga cgctcaacgc
      661 ctccaagggg cagctctccg acaagatcga cttcatcctt ccctcggaca aggcgaacta
      721 cgactaccag atcgactggc gcctgaaggg caacaagacc gtctcgtcgg gccggaagag
      781 cgcgagcgac gcgatcctct tcgtcgacga agtccccgcg ggttgaggag gaagccatga
      841 gacgaaccac gatcgttcgc atcgcggcgc cgctcttcac cgcggcgctc ctcctgtcgg
      901 cttccgcggc cagcgcgcag accgaccggg actatctcaa gaacaagctc tcgttccgct
      961 acgtgggcac gccgaacaac ctctcgaccg cggtcgaggt gctggacaac gcggcggtgg
     1021 cctcgggcac cgagaacggc accttctggg cctcccccgc cctgagcgga cttcagggaa
     1081 tggtgcgcaa gctgctgaag gccccaggcc aggggggcga cgccaatctc cagtacgtgg
     1141 tcgccaagac ggtcaagatc caggaccgcc cggtgatgat cctcatggtg aacgacgtca
     1201 cggcgccgct gtccgcggac gccatgaacc actgggacgc ctgcgacgac ggcgccggcc
     1261 acgcgtggcc gtgcgcctcg aacgcctcca ccaccgacga ccagcggcag cagtgcgcgg
     1321 agtcgcggca ccagccggtg ccggcccggc tggacgcgac gtgggcgggc cagatgacgc
     1381 tgggccaggc ggcgttcaac ggaggcaccg cggggagcgc caccggcacc ttcgtgcacg
     1441 agctggtgca cacccaggac cgctccgacc gccgggcgca catgttcacg atcagccgga
     1501 ggtcgtacca ctacggctcg gacggcaccc attacgacat cgaagcggtc ccgaacctgg
     1561 cctcgacgta ccaggaaggg atcgcgaacg cggtcatgat gacggtggac acgcgcgagg
     1621 cgcagcgcct gttcgactgg ttcgcgaaca acgacgtcat gatggtcgag aagaacgttc
     1681 ccgtgccccc gggcaccgga gcgggcgcgg ccccctgctg gaccgtggtc acgacgccct
     1741 cggcggacat ctggctctac aaccagctca cggcggccca cgtccgggaa gtgcagcgca
     1801 cgcccaaccc ctatcccaac tacgcctact tccgcatccg ggacctgccg ccccggttca
     1861 tcgtgcacaa cgagaacatc atcgcgctcg tcttctccga gtacgcgcgg cacctgggcc
     1921 tgcagaagtt catggacgcg ctgaagacga acgaccggac gatcttccgc gtctccacca
     1981 gcccgatcgc ccagctctac aatacgctct gccgcgccgg cctcgaggga cgcccgctgt
     2041 cggccgtgac cggggtgaac gaggcgggac ccaagccgta cctgatcccg ctggcgtacg
     2101 ccgactattt cacggcctat cgctcccagt ccaaggacga ctacaagacg atcttcgaga
     2161 acatgctgcc ccaggagtgg atcgacctct actgggacgg ctacaaggat gccgttcgga
     2221 gcgcggtgcc gatcgacgcg acccacacgc cgcagttcac gaacctgacc gacatctcga
     2281 tcgcgctcgg cgtgaaccag tcgaccgcgg aacattgaga aagggacttc cacccatgat
     2341 cgttcttccg cgcgggtctt gcccgctcct cgcggccgcc ctcttgttgg cgatcgccat
     2401 ggcccttccc gctcccgcat cggcaggcga cgcggaccgg acctacctcc ggtccaagct
     2461 cgtcttctac tacgtggacc agccgaaccg gaccgacctg cctccggaga tcctgaacaa
     2521 caccgcgatc tcgaacggga cggagacggg cggattctgg accagcacca aagtccgcgg
     2581 gctccaggga ctcgtgcgat ccctcctgca ggcccccggc catggcggag acgattacat
     2641 gcagatggtg accgcgtgga tcatcaaggt gctcgacaag cccgtgatga tgaccctcgt
     2701 caacgacgtc gacgcgccgc tctccagcgc ggccatggac cggtgggatg cctgcgacga
     2761 cgggaacgga catgcctggc cgtgtgcttc caacatggcg accagcgacg attaccgggc
     2821 ccggtgcgcc aagcagaacg gcacctcggc gcccgctcgc cgcgacttct gggcgggcca
     2881 gatgacgctg ggccaggcgg tgttcaacgg ctccaccgcc ggcaaggcga cggggacgtt
     2941 catccacgag ctggtgcata cccaggaccg gtccgaccgt caggatgtgc gtttctggct
     3001 gagccacacg gcctacagct acggcgcgga cggaacccac tactcggtcg aggcggtccc
     3061 gaaccttcgc gcctcgtacc aggaggggat cgcgaacgcg atgcgcctcg cggtggacat
     3121 ggaccgccgc aaggagatgt tcacctggtt cgcgaacaac gacgtggtgc tggtggaaaa
     3181 ggccctcccg acgcgcagcg tctatctgaa cgatcatccc tgcgcgagca tgtgggagtt
     3241 tccgtcgcag gacatctggc tctacgacca gctcaaggcg gcgggcgcgc acgaggtgac
     3301 gagcacgacg cccatcccgg ggtatgcgca gtaccagatc cgctccatcc cgccccggtt
     3361 catcgtccac aacgaataca tcacgtcgct gaccatggcc gaatacgcct ggcacctcgg
     3421 actgggcaag gtgctccgcg ccatcaagac gaacgacgcc gcgctcttcc gcacggcgaa
     3481 cagtcccatc gccaagctct acgagaccct ctgcacgatg ggactcgagg gacgtccgct
     3541 cgcttccgtc cagaacgtga acgaggcggg gcccaagccc tatctcatcc ccctcgcgta
     3601 cgcggacttc ttcaccggat accaatcggc gaccaaggcc gattacgcat cgatcttcga
     3661 gaacaagctg ccgcaggcct gggtggacat gtactgggac ggctacaagg acgcggtgcg
     3721 ttccgcggcg gtcatcgacg cctcgcacaa gccgcggttc gaccagctga ccgacatcgc
     3781 catcgcgctg ggcgtcaacc aatcggtggc cgacgaggct ccgtgacccg aacgccgcac
     3841 ctctggagga gttcgatcat gaaagcggta ccgacctgtc gcgcgctcgc cgcgctcctt
     3901 ctgctgtcgc tcgcggtttc gggcggcgga ttccgaagcg cacgagccga ggacgcctcc
     3961 gcggcgccgg cggcggccgg tgacgcgtcg tcggcctccc cggtgagcgc caccgggctc
     4021 ctccccatct tcggcgtcgc ggtcgatttc gacgccctcc cgaacaacgc caagcccgac
     4081 gacgcggtcg cgtcggtgca gaagctgtgg gacgcccgtc tcaaagaggc cggcttcaac
     4141 gtgatccagt tccccgtgga tgcccgggag ctgggcgaca agggagcggc gcgcctggcc
     4201 aagctgtgcg cgtgggccaa gacgaacaac gtgcggctgg cgccggccct cctgggtgct
     4261 tcgcccggcg aggcgctgcc gtcggactat cccgacaagg tcggggcgtt cgcgacgaag
     4321 acgatcgagc tggtcggcaa gggcgatccc gcggcctacg cgcagatcat gttctaccag
     4381 ctggagcggc cgctgaacca cccggggaac cacggtccca tggacgcggc gcaggcggcg
     4441 tcgctgatcg cgaacgccgt gacgaagctg cacgcggcgg agcaggccgc gctggccggc
     4501 tcgtcgctcc aggcgacgcc cgttctcgcc ggcgtgtcgt tcgactacga gctgatcaag
     4561 gtcggcgcga tcgccaacgt tccgatcacc gacgagagct accagcaggc gtacgcctcg
     4621 tcgcacgact acctggccgc ggtgctgggc gcggcgccgg tggacgtcgt cgggctggag
     4681 tggttcccgg gcagcttgag ctcggacggc gtggaccgct tccccgatct gatcggcaag
     4741 ctgcagggcg acttccccgg gaagctcctc atcgtcagca ccggctactc caccgcggcg
     4801 aagggagagg ccgaccagtc ccgctactac acgcagacct tcaacaacct gagcgatctc
     4861 cggacgaacc agggggtgga atcctccttc gcggggatcc tctggcgcac cgccatggac
     4921 aagccgggcg gcgagcccgc gcctccctcc tccaagacgc tcgacgagat ggcctcgtgg
     4981 aactggcagg atcgcgccgc cgagctgacg cgcatgtgga acgagcccgg atcggactcc
     5041 aaggagatgc gctggtgggt cgggcgcgta gagagccggt tcgggctggt gggctcgagc
     5101 aaggacgctt cgaagccggg aatgcccaag gcggcgtgcg cgctcctcgc ccatctcaag
     5161 acctcgctcg cggcggcggc gatgagcacg ggggcggggc agatcgcgga ggagctgacg
     5221 gcggccgcgt cgggaccggg cgggaccaag gggctcgtcg gggcggtcaa ggacaagctg
     5281 caggccgcgc tcttcggaat gctcgacgtc tgggtggcga agacggccga gagcctcgtg
     5341 agcggtggcg gcggcggcgg tgacggcggt ggcggtggcg gcggaggcgg tggcggagcg
     5401 ccgccgcctc cccccccgcc cgcgccgacg aacaccgccg acatctacat cgccggcgcg
     5461 tcggtggaca gtgagatcaa ggccggatcg ccggtctcga tctcggtcac ggtcggaaac
     5521 ggcgggcccg cgacggcgac cggcgtgtcg ctctacctga agcagaacaa cggcacgagc
     5581 gacctgacgc agtccacgcc gacctcgatc acgccgggct cctcgcaggt ggtcacgctc
     5641 aactggacgc cggaccaccc cgggaactac ccgcacgtca ccgtggaagc ctactgcgac
     5701 aacgattccc agctggccaa caacaagacc gatcccgtgg acatggacgc ggccccgggt
     5761 ggcggcggag gaggaggagg cggcggcggc ggcggcggcg gcgcgggcgg cctcggtggg
     5821 ggcatgggcg gcaagctcgg cggcaagtac ggcggcgtca tgcaggtcga ccccaaggtg
     5881 ctcggcggcg tgaaggggaa tctcgtcacg aagaccgatc ccggattcat tcacatcgag
     5941 gcggtgcgcg gcatcagccc gatgccgatg atgaacacga tgtcggcgcc cggtgcgggc
     6001 ggcggcacgc ccatggcgat gcggatgggc acgggcaccg gaggcggcac cgccaaggcg
     6061 ggcggtggcg tcgccatgat cggcggcgcg ggcgccgcga cgggaggcac cggcggtgcg
     6121 ggaggcggaa ccggcggcgg cacgcagacc atggcctacc gcatggaggc ggcaccgagc
     6181 gggccgcagc cggtcgcgct cgcgttcacc gtaacgaacc ccttcgacca cacgttcacg
     6241 tcggtcaagg gaacgctgaa aattcaggac aaggtcatcg cgaccaagga tctcggggtc
     6301 ctcttcccgc ggcagcgccg taccgtgtcc ttcgcgcagt ggacgccgcc gaagacggga
     6361 acgttcccgg tccggatcga tctcgccggt ctcggaccgg gcgggaagcc cctgagcgcc
     6421 tcggcgatgg accagatcac ggtgaacccc ggcggcggcg cggcgccccc cacggggacg
     6481 gccacccgca tggtgatcgg cggggccggc gcgcgcggcg gcacgtccgg cggcgccgtg
     6541 ccggtctcga tgacgcgcgg acccggcacg ctgacgcaga cgcggctcct cacgccgctc
     6601 atcatggcgc ccgcggcgcc ggcgatcgcg ggcccggcga cgcgctcgct ctacctcgcc
     6661 ccgggcggaa cgcgccccat cggcgggatg gcctcgatcc tggggctcac gccgaacagc
     6721 gtcctgctcg ctcccttccc ggtccaggcc ggcaacgacg tctcgatcac gatccgtctc
     6781 ttcaacaccg agcgcacggc ggcgaagaag gtgaaggtgg aagcctacgt cgacggcgac
     6841 aagctgggcg agaccaccct cgacgtgccg gtggcgcggg cggtcatcgc gagcggcttc
     6901 ccgacgtgga aggcgaagcc cgggcggcat gacgtgcgcg ccgtcgtcac cgccggagac
     6961 aggagcggcg ccgcctccaa gccggtcgac gtgcggcctc cgaacggcgt gggcgcgggc
     7021 ggcgtggcga tgctcatggg gcggggcgcg ttcacgctct cgaccctgca gatgaccacc
     7081 gccgacgtcc ggctgaatcc cctcgccccg gcggtcggaa acgcggtgga cgtctcggta
     7141 cgcgtccaga acgcgggcgc cgcggacgtg aaaggggttc gcgtggagct cttcgccgac
     7201 ggcacgcgcc tgggcgaagt gtcggggaac atcgccgccg ggaaggattt cgtcttcagc
     7261 ggcttcccca agtggacggc cgcctcgggc gcgcacaagc tgctcctccg cgcgagcgcc
     7321 ggcggacgga tcgtcgaggc gacgcgcgac gtggccgtgg gcgcggcgat catgctggcc
     7381 aagccgctcc tgaccacggc gacgctcgtc acgcccacca cgccggcggc tccgccgtcc
     7441 acgcagaccc cggtcgtggg gcttcggacg acggcggtca ccgggatgac cctgatgatg
     7501 gtggcgaatc cggatcttca agtcgcgccg ggcgacatca cgttcctgcc gatctctccc
     7561 aaagcgggcg acccgctgac catcaacatc acggtccgga acgtgggcac cggcgcggcg
     7621 aacaacgcca cggtgcgcgc cattctccag gcggacggag ccgaggtaac ccgccgcgag
     7681 ttccccgtca acctggcggc gtccggcatg accagccttt cctggccggt gaccacgcct
     7741 tccggaaagg tgctgaccgc ggtggtcacc gcgatcgtcg ccaacgatgc gcgtgcggac
     7801 aacaaccagg ctcaggccac gacgtcggtg gcgatcgctc tacagcggca gctcagtccc
     7861 atgatcctgc aggttcagcc caagtaaagg ggaggccaat cgcatgtctc gtgtcgcgcg
     7921 cgccgggaac gtctcggcct ggctcagcgg tctcgttctc gtcgcgtcgc tcgcgccccc
     7981 atcgttggcg caaggcgtgc tcagtgggtt catgaacccc attggactcg cctcctccta
     8041 ccccgcggat tggcagcgga cgaactcctt cgcgtccatg cagctcttca acaacgcaca
     8101 ggagccgctc acggccgaca tcaagatcac gctggtgcga aatgggaccg aggtggccac
     8161 gaccccgccg gtgcagcggc tctaccccgt gggcggcttc gcagtcccca ctcccgacct
     8221 cacgcggtgg cagtcgatgc agttcgctgg gaaggtaggc gacgcggtcg acctgaccgg
     8281 ccgcctcccg gacggggtat acacggtctg cgtcgatatc tcgaacgtga gggggaccgc
     8341 gacaggaacc ctgtacaacg ccggctccga ggtctcccag tgttcccagt tcatctcctt
     8401 cttccccaag cccccgggcc tgatcgcgcc ccagagcgga agcgtcgtgt ccacgccgac
     8461 gccggtgttc caatggacgc cagtgctctc caacctaggg gcgcagctcc cgcacctctt
     8521 ccgcctggtc gaggtctatc cggggcagac cccggagcgg gccatcgagg cgaaccggtc
     8581 gctctacgaa caggtcttcg gactcaacat gccgggcggg gacctgagca gcttcgccta
     8641 tcccgcatcc gcgccctacc tcttgagcgg ccaccgctac gcgtggcgcg tgcaggcgtt
     8701 cctcacggtc ggcacctcgg ggaacggagg cggcatcagc acgggcctct tcgccaagcg
     8761 agccgtgatc acccagtaca acgcccgcgc tctcggcgag aacgagggcc gcagcaaggt
     8821 ctacaccttc acttggaatt cggaccccct cgcggccgcc agggcccccg cagcgcagga
     8881 cagccccgcg agtgaggacg cccggatggg caacgtctgg agcccgtcgg gcaatggggc
     8941 ttcggcgcgc gaggccgatg ggttaggcga cgacgcgttg gatgccgatt cggacgctcg
     9001 tcaaagtgcc tcgggagagg cgggacagag cgaggggaac aactttgccg accggctcgc
     9061 gcgcatgatg gcctcgctct ggaagcaggg tccgggcagc gcggtgtcgg tggcgcgggc
     9121 cggcggaaag ggcgcgctca cgccctccgg cgctcccgag cgcgtttcca tgacgaacgg
     9181 ggcaagcgca cgctacgcac cgggagatgg ccgccatcgc ccgacgtccg accccagtgc
     9241 cagcgcccag ggacagcccg cggcgaccga gacggctcag gcgaccgatg ccggccccgt
     9301 cgacagcacc gcccaggccg tggtcacccc tccccccgca cccgcggcgc cgccgtccag
     9361 cgagagcgcg ggactaggtc ccgggtgggc acgcctgcac gggacggcgt cggtgagcgg
     9421 cgagacctac agcagcgacg gcctatcgat cccgaaccgt cccgaccgca gctcgcgtgt
     9481 cgtctccggg ctcagcgtcg gcgtgatcca ggaccggatg aggatccccg tctcggctct
     9541 cttctccgac gaccaggtcg cgtttcgcca gaacatcaat caggtggcgg tggcgccgcg
     9601 ctttcgatgg gccgggctca ccgcgggcaa cttctcgccg cagttcggca gctatacgct
     9661 ggcggacgcg acgatcctgg ggggcggtct cgagctcgcc cccaagaact ggcacctcgg
     9721 attcgtgagc gggcgcgcac gcaagtcgat cacgcaggtc tcgagcgcct tgggcatcat
     9781 tccgcagctc gagcggaaca tgaccgcggg ccggatcggc tacgggaacc cgctcaccaa
     9841 ctcggtcgag ttctcgatcc ttcgcgccac cgacgatccg aagtcgatcg cgggcaccga
     9901 caccctgtcg acccccgagg ggaacacggt gtattccgtg cgcgcgcagg ggctcatgcc
     9961 cacccgccac ctgcgggcca tcgtggagac cgccctttcg cgctacgacc gcgaccggag
    10021 agcggacgcc tcatcggtcg acggccaggc gctgggcctg cagctcttcc acgagaccgc
    10081 catgacccgg atcggagtga agaccgagta cctgaacggc ggcttcatga cgctcgggaa
    10141 ctcgggaatc accggagatc gagtggacgt gggcgtggac gcgcacgtcg cgttcctcca
    10201 gggcaagctg aacctggacg gcatggccgg tcttcgcaac gacgccgtaa gcgaggctct
    10261 cgcggccgag acacgccggc gcaactacgg gctcacgggc tcgtggcagc cggggccgcg
    10321 cttcggcgcc gacgcccaga tgtcgatcta ctccagcgag tcggacgcgt ccgactcgct
    10381 cttcctgggg aacagcaaca ccacccgcgt ctactcggtg gcgccccgca cgatgtggac
    10441 gctcgggaag gtacagaact cggtgacctg ctccgcgacc atccagaagt cggagaatgg
    10501 cggcccgatc gccgacacga agagcctctc gttgctcggc aactacgcgg cccaggtggg
    10561 cagtccgctc gcgatcacct tctccggtag ctacaccagg accgatttcg agatcgcggt
    10621 gagcgagctg agcacgttcg gccccggctt cacgctgaat tcgttcggat cgaagctctt
    10681 caccaacgcg cagctccagg tgacgaagac gcgcaccggg aacgcgggca ccgacaccga
    10741 cctcaccccg cgggtggaga tgcggtggaa cttcgcgcag cggcagtccc tcatcctgcg
    10801 cggcaacttc cggcgctacc agtatgcgat tcccgggacg cccgagttca acgagcggat
    10861 cgcgtcgctg gagtacgtta cgaatctata aaccgccggc gccagccggc ggaaggcggg
    10921 gcggggaggc caagcttccc cgcccatttt ttgggccgcg agcctcggtc ccgcctccgg
    10981 tttgtggtac cgtccctcga tgcccgaccc tatcccgttc catcgcccct cgatcaccga
    11041 cgccgaggtg gacgcggtca cggcgaccct ccgctccggc tggctgacca cgggaccgcg
    11101 cgtgcgcgac ctggaacaga tggtcgccga ctacgtcggc gtgcatcacg ccgtcgcgct
    11161 ctcctcctgc accgccgcgc tccatctggc cctgatcgcg gcgggggtcg gccccggcga
    11221 tgaggtcgtc acctccccgt acacgttcgt gtccaccggc gagtcgatcc tctacctcgg
    11281 cgccaagccg gtcttcgtgg acgtggagcg cgcgaccaag aacctgaacc ccaagctcgt
    11341 ggcctccgcc atcacgctga aaacccgcgc catcgtgtcg gtatcgatcg cgggccaccc
    11401 ctgccacgcg caggagatcg cggccgaggc caagcgccac gcgatccccg tgatcgagga
    11461 tgccgcgcat tccctgaccg cccggatcgg cgatcagccg gtgggacgcc aggcggacat
    11521 gacctgcttt tcgttctacg cgacgaaggg ggtcaccgcg ggcgaggggg ggatggtcgt
    11581 caccgagcgc gccgagtggg ccgatcgcgt ccggcggctc tcgctccacg ggctctcggc
    11641 ggcggcgtgg tcgcgctatg acaagggcgg ctggtgggac tacgacgtgg tcgagctggg
    11701 ctacaagtac aacctgaccg acgtgcaggc cgcgctggcg ctggcgcaga tgaggcgcgc
    11761 cgacgcgatg cgcgaccggc gcgaggcgat cgcggggcgc tacacggagg cgctcgccgg
    11821 ggtacggggg cttgcgacgc ccagcgtcct gcccggcgtg cggcatgcgt ggcacctcta
    11881 ccagatcgag atcgcggagg gcggacgcac cgaccggaac gcgctcgcgc tcgcgctacg
    11941 cgaggatgga atcggcacca gcgtccactt caagccgctc cactgcttcc cctggtacca
    12001 ggagcggctg ggggtgcggc cggggcagtt ccccgccgcg gagagctgct acgcgggcac
    12061 gttatcgctg ccgatctggc ccgacatgac cgacgcgcag gtggatcgcg tggcggaacg
    12121 gatccggaaa cacatgtcgg ccgcttgagg catcatggcc ctaccagcga gtttatgttt
    12181 tcccgataaa ctcgctaaat tcgctataaa tccccccgaa gctttggatc gcgtcatcaa
    12241 ggccgcttct tacatgttct agaacatgtt cgttgtgccg tgctgacacg ctcttgccgt
    12301 atgtagcgaa tgattagcgg catccgggca aacgcattcc agcgaagggc ccgtcatggc
    12361 cgcggtcatc accgtagcgc ctgacgcagc atcgccacaa tccgctccgc cgccttaccg
    12421 tctccatagg gattggcgcc cgtcgccatc gcggcgtagg ccttggggtc gtccaggagg
    12481 cgcgacgcct cggtgacgat cttctcgcga tgcggcccca ccagcttcac gacccccgcc
    12541 tccaccgcct cggggcgctc cgtctcgtcg cgaagcacca gcaccggctt cttcaaggaa
    12601 ggggcctctt cctggatgcc ccccgaatcg gtgaggatca ggtgcgacgc ggccatcagg
    12661 tcggagaagg ccttgtactc gagcggctcg atcaggtgca ccccggcctc ggaaccgagc
    12721 gcctcgcgcg cggggcccgc cacgttcggg ttggggtgga cgggatagac cacgtccacg
    12781 tcggcatacc gcttcacgag gtcgtgaacg gcggcgaaga tctcctgcat cggcgccccg
    12841 aagttctccc ggcgatgcgc ggtcaggagc acgatccgct tcccccgcaa cggcagcgcc
    12901 ggcgcgcggg gcggttgcat cgcgaccacc tcgagaagcg cgtcgatgac ggtgttgccc
    12961 gtgaccacga cctgcccctc ggcgatcccc tcgcgcagga ggttctcccg cgcgcggcgc
    13021 gtcggcgcga agtggcgcgc ggcgatcacc gacgtgacgc gccggttcac ctcttcggga
    13081 tagggataca ggttatcgtg cgtgcgaagg cccgcctcca cgtgcccgac ggggatcttc
    13141 ctgtagaagg cggcgaggga ggcggccatc gtggtggtgg tgtcgccctg gaccagcagg
    13201 aagtcggggt tcgtctcggc caggtagctc tcgagacggg tcagcgctcc gaccgtgatc
    13261 tcggtgaggg tctgtccctc ccgcatgaga tccaggtcgt ggtcgggaac gatcccgaag
    13321 agtccgagca cctgatccag catctgacgg tgctgggcgg tgacgcagac caccgagcgg
    13381 attcccgatt cgcgcgccaa ggccttcagc accggggcca tcttgatggc ctcggggcgg
    13441 gtcccgaaga cgctgaggat ggtcttcacc ggggccagtt agccacaggg gtctgtaccc
    13501 gtcaacggat tccgagcccc cgtatgttac agtcccatca tgaacttcca ccgatccggc
    13561 ccgtcccgtt cccggctcga tccatgacct ccgtcgggga gaagctgcag gccgcgctcg
    13621 gagagcgcta tcgggtgacg cgcgagctga accggggcgg catgggcacc gtgttcgagg
    13681 ccgaggatac caaacaccgg cgcgcggtcg cgatcaaggt gctgaacccg gagctgtccg
    13741 aggtgatcgg tccacgccgc ttccaggccg agatcgagat ggccgcgcgg ctcatgcatc
    13801 cgcacatcgt gccgctgcac gattcgggat ccgggaacgg cctcctgtac tacgtgatgc
    13861 cgctcgtcac gggcgagtcg cttcgccagc ggctgacccg cgagaagcag ctccccgtgg
    13921 aggacgccct ccggatcgcc cgcgaggtgg ccgacgcgct ccagtacgca cacgcgcacg
    13981 ggctggtgca ccgcgacatc aagcccgaga acatcgtcct ctcgagcgga cacgcgctgg
    14041 tcctcgattt cggcgtggcg cagtcgtcgg ccccgggggc ggaagcggtg acgcgaaccc
    14101 tggcggttct cggaacgccc ctctacatga gccccgagca gtccgccggc ggcgccgtgg
    14161 acgcgcggag cgatcaatac agccttgcct gcgtcctcta cgagatgctc gcgggccagc
    14221 cgccgttcac cggcgggacg gcggacagca tcggtctcca gcatcggacg ctggagcctc
    14281 gcgcggtcac gaccctgcgc gccagcgtgc cgccgccgat cgcgctggcg ctcgcacgag
    14341 cgctgtccaa ggctccggcc gatcgcttcc ccactgcggc cgcgttcggc gagtcgttgg
    14401 ccgtgctcgc cacgccgtcg ctggtgccca cgccgggggg cccgcgacgc gacgccacca
    14461 cccccgggcg gccccccggg ggacgcgtga tgctggcggt tctcccgctc gagaacatga
    14521 gcgccgatcc ggagcaggag ttcttcaccg acgggatgac cgaggagctg atcgcgcatc
    14581 tgggacgcct ccagcccaag cggctggggg tgatcgcgcg aacgtcggcc atgcgctaca
    14641 agaagacgac caagagcgtc gaggagatcg gccacgagct gggcgtcgac cacgttctgg
    14701 aggggagcgt gcgccgcgcc ggagaacggc tccggatcac ggtgcagctg atccaggtcg
    14761 ccgaccagac ccacctctgg gcggagaact acgaccggag gatggcggac gtgttcgaca
    14821 tccagagcga ggtcgcggcg agcgtcgcga aggcgctgga agtgcagctc cttccttccg
    14881 gccgcgccgc cgggtcggcg ggagcggcgg cgaatcccgc cgcctacgag gcgtatctgc
    14941 gcggccgctt tcactggaac cgccgcacga aggagggttt tcgcctcgcc atcgaggcgt
    15001 tcgagcaggc cctcgccgcc gatccggact atgcacgggc ccacgccgga ctggccgacg
    15061 tctactccgt gctcccgaac tgggactcga tcaccttcgc cgaaaccgct cccaaggccg
    15121 aggcccacgc caagcgggcg ctcgagctcg accccgacct ggccgaagcc cacgcctcgt
    15181 acggcgccgt tctcaccaac ttctatcggg actatgtcga ggccgagcgc gaggaccgcc
    15241 gcgcgatcga gctcgaccct tcgtatgggc tcgcctatac gtggtgcgcc acggtgctga
    15301 tggcccaggg acgaatcgac gacgccatcg ccatgaacga gcgggcccac cggctggacc
    15361 cccaatccct ggtcgtgctg atcaacttcg gcgcaatgta tttctggaag cgggatttcg
    15421 agcgcgccgc ggcctacacg ctgcgcgcgc tcgagatgga gccctcgctc acgcgccacg
    15481 cggccttcgt cgggatctac cggggactgc agggacgcgt ggaagagggg atcgcctacc
    15541 tctcgcgctt gagcccggag cagcgccagg acccgcacgt gatggcagcc atcggcttcc
    15601 tgaacgcgaa ggcgggccgg ccggagccgg ctcgggaggc gctccggcag ctggacgagc
    15661 gcgcgaagag cgaggcggtc ccctacgtcc cgatccattt cctgctcgtc gcgctggacg
    15721 agcgggcgcg cctgtatgcc tcgctggaga agcgcgtcgg cgagggaacg attccgccgg
    15781 tgacgctgcg ctacgcgccg acgttcgacc cgttccgcga agatccggag ttccaggcgc
    15841 tgatggcccg caccggggcc tgaggattac ggcgcaagcg gctccgccgg gcgctgccgc
    15901 ggcaacgaga agctgaacgt ggagccccgg cccagggccg actccaccca gatcttgccg
    15961 ccgtgctgct cgacgatccc cttcgagatc acgagcccca gccccgtgcc gcccgccttg
    16021 cgcgtcgtgg aggagtcgag ctgggcgaag cgctggaaga ggcggttcaa gtcccgctgc
    16081 gcgatccccc ggccgtagtc cttcacggag atcgtgacgg tggaggagtc gcccgaggcg
    16141 aacacctcga tgcgcccctt gtcgggggag aacttgatgg cgttcccgat caggttggtg
    16201 gccacctgcc cgatgcgcat gggatcggcc tcgaccgact ccgcgctcgg gtcgatcttc
    16261 acctccagct ccaggccgcg gctggcggcc aggctccgga tgttctccgc cgcctcgacc
    16321 agcacgcgcc ccacgtccat ccgctcgaag ttgagcgaga ggcgcgagga ttcgagcttg
    16381 gagaagtcga ggatgtcgtt gatgagggag atgagccgct gggtgttggc ctggcagatc
    16441 ccgagcagct ccttctgcgg cgcgggcagc ttgtggaagc gctcgtcgcc caacagctcc
    16501 agcgagccgt tgatcgaggt gagcggggtg cgcacctcgt ggctcacgac cgcaacgaac
    16561 tccgacttca ggcggtccag ctccttcgtc cgctcgtaga ggatggcgtt ggtgaccgag
    16621 atcgccgcct ggctcgcgag catcttgagc ccctcggcgt cgtcggcggt gaactcgtcg
    16681 ccgtcggcga gattgatcgc ggtgatcgtc ccgatgacgc tctcgttcga gaggagcggc
    16741 acgatcaggg cgttctccgc gtgcagcgcg gcgagaacct cctccacccc gaccgcctcg
    16801 gagatgccgg tcaccagggc cggctcggcg ctctggacca cctggccgtg gaagcccacc
    16861 cccatcgcgt accggagctt ctggtagcgg tcggggggga ccccgatccc ggcgatcgcc
    16921 tcgaactcgt ccgagccctg gtcgtgcagg aagaggagac aggcccgggc gcccgtgatg
    16981 cgcgccgcct gggccgcgac cacgtcgcag gtcgcctgga ggctcaggct caggctcatc
    17041 gcctttcccg cctcgtacag cgtgccgagg cggtaggtgg cgcgctcgac cttccggcgg
    17101 aggatctcct cgtgctcctg gagctcggcg ttcgcccgct ccagctccac gatgaggcgc
    17161 cggttctcga ggatgagatt ccggctctcc aggccgcgct cgatcgcctt ggccgtctcc
    17221 cagagatcga acggcttggt gatgtagtcg taggccccct ggcggagcgc gtcgatggcc
    17281 gtggtcgtcg aggcgtagcc ggtgatcagg atgaccaccg tgtccttgtc cgccgccttc
    17341 gcggcccgca cgacctccag gccgtctacg cccgggagat tgatgtcggt gaggaccagg
    17401 tcgaaggccc ccgtcgtcac cgtcgtgatg gcgtcgtccc cgttgtcgca cgacacgacg
    17461 tcgtacccct gctgggtcag caggtcgcgg aagatctgga cgacctggtc ctcgtcgtcc
    17521 acgaccagga tgcgcgcctt cttctccggc gcaagatccg ccgcggggac gggagcggcc
    17581 acgggcttcg ggttcatcgg gctcctggaa tcgggttcgt gatgcgacgc ctcaatgcgc
    17641 cgcggcctcg tcgagcggtc cgcgcgtccc ggccgggatc gcggccgtct ccgccacgcc
    17701 ccgcttggcg gcgaggagcg cccgctcggc gcgggtgttc agcgatccgg agtcctgcgc
    17761 atcgaccggg aaggtcgcca cgccgatcga gaccgtgatc ttccggcggc gcgcggtcgc
    17821 ctgcttctcg accagcgtgc gcagccgctc ggcggcgatc atcgcctcgg gcgtgtcggt
    17881 gtggggcagg aggatccgga actcgtcggc gccgtagcgc gacacgcagt cgctgccgcg
    17941 ggccgcccgc ttcagggcct tccccatgtc ggagagcacc tgggtgttca ccgtgccggc
    18001 ccgctcgcgc gcgcccgtgg cgttcacgtc cagcaccacg accgacagct tgtggccgta
    18061 gcgcttcgcc ttcccgacct cgctctcgag cgaccggtcg aacgcccgct tgttgaagag
    18121 ggtcgtcacc tcgtcggtgg tgcccagccg ctccgcctcg cgcatcagga tcaggcggat
    18181 caccatcgcg gacaccggca gcatcatcag ggcgatccgg ctcgcgctct cctcgtcgaa
    18241 cgcgcccggg cgggagtggc tcaggttcac gacgccgacg agatctccct ggaccagcac
    18301 gggaaccgag aggaaggagc gcacggtcgg ggcctccgag cccccctcgc gatggaggtc
    18361 gttcaggagg accggcttcc gcgactgcgc gacccaggac gaaaagcccg cgccgcgatc
    18421 gaagcgcaca tggccgatca ggtcgatccg gtcgcccacc gtggcggcct cgaccagcgt
    18481 cccgttgtcg cggtcgagca cgaagagggt ggcgttctcg aacggcacgg cccgctgcac
    18541 ctggccgagc gcggcggcga aggcgtcggc gggagcggcg ctccgctgcg cgatggcgct
    18601 caattcgccc atcacctcgg cgtcggagac ccgcgccttg gcctcgctcc ccttcccctt
    18661 cccgagcacg gccatgactt tccggttgat cgcgatggcg ctggcgaaga cgtcggagga
    18721 gcgcttcttc gccgcggaga gggcgaggtc cttcgcgaag tgctcgcact cgttcttctt
    18781 caagatccgc cggcccccca gcacctcgag gagcttctgc tccgagcgga tgaaccgttc
    18841 cgtcgcctcg accagccgct tcgaccccgc cccgttccgc tgcttcatga cttcctccct
    18901 ggaaagctca ccgcgccccg ggcgcgtctc cctgatgacg ggccgatccc ggcaccaccg
    18961 ccgaatccac gatcgtgacg cgggccccgc cctcgtcccg gcccctgccc gccggcgcca
    19021 gcaccatcgg cggtcccagc tccttcacgg tgaccgggaa ggtctccccc ggcttccact
    19081 ccggcgcgcc gtgcgccatc agcgtgactc catggaaatg cagcagcgcc ttcccccgcc
    19141 cgagaatccg ggcgacggtc agcggctccg tcgctccttc gtgcagcgac gcgtagagcg
    19201 ccgcacccag ccccggctcg aaacggatcc ggatctcgac cgggcggctc gcgctcggct
    19261 tggcggcggt ggaccccgtc ctcacccaac ccacttcgtt cactcctctc ccgatccgcg
    19321 gaactcggcg aagagcgctt tctcccgctc cgtttcccgg atcacctcgg agaccagctc
    19381 cgcggccagc gcctcgcggc gcgccgcgcg cggctccacc ggctcccacg cgcgctcgtc
    19441 gagcccctgg gcgtcgatac ccgacgccca ggggtagccg gcgcgctgcg cgaggaagtt
    19501 ggcgatgtgg accagggaag ccagcgcgac gttccgcgcc gccgtctccg gccggtgatg
    19561 gcaggcgatc gcctcgacga tgtcggaggg gagtccccac cgctcggcga gccatccgcc
    19621 cacctcggcg tgatccgatc cgaagtgcgc gcgttccgcc tcgaaggagg ccacgccctt
    19681 ggtgcggcag cgggccagga ccttctcgaa gcgcgcggga tcggactcgc gcagcacgac
    19741 cttccccacg tcgtggagga ggccggccgc gtaggcgtcg ccggtcaggc ggtacttcgc
    19801 ccgttcggcc agcgcccgcg cggcgatgcc ggcggccatg gagtgcgcaa ggagcccttc
    19861 cagggaggag cccgggcgtc ccagcgcccg caccaccgac gacgtgagca ccaggtcgcg
    19921 gatcgtggag gggcccagga ccaggacggc cagctccacc gagccgacct tccgcgggaa
    19981 gccgtagaag tcggagttcg ccaggcgaag cgtgcgcgcc gtgagcgcgg gatcgagcgc
    20041 caggatccgt ccgatctcct cggaggtcgc ttcgggacgc gccacttctt cgatcaggcg
    20101 cgacgcgacc gccgggaagg tcggaaggga gacggtgcgc tccgccgcgc gctggagcgc
    20161 catgcggcgc gagattccgg ccggctcggc atggacgcgg gcgcgggcgc gggcggcgga
    20221 acgggcggcg gcgacccggg cgggggcgcc ggagaggtgc gccatcaaag cacccgggcg
    20281 agccgcgcct tcagcgtctt cacggcgcgc gtgtggatct gcgagacgcg ggattcggag
    20341 atatggaggg cttcgccgat ctccttcagc gtcatctctt cgtagtagta gagcgcgacg
    20401 accagccgct gctgctccgg aaggccgtcg atcgtctcga gcagcacctc gcgcgcctgc
    20461 tcgctctcga tcacttcgag ggcgtcggcc tgcgcggggt cgtccacgat gtcctcgagc
    20521 tggatgtagt cctggtcgtc ttcggtcgag accgctttcg agagggagag gagcgccgtc
    20581 ccgtgcacct cgtccatgag gcgcgacagc tcgaccgggc tcaggttcag ggcgtcggcg
    20641 acctcttcct cggtcgcggc gcgtcccagc ttctgtccca gctcgcgggc ggcctcttcc
    20701 accgtgcgcg ccttccgccg gatggagcgg gaggcccagt ccagggaacg cagctcgtcc
    20761 aggatggcgc cgcggatccg ccagatggcg taggtctcga acttggtgcc cttcgaggcg
    20821 tcgaacttct cgagcgcgtc gaagaggccg atgatcgcgg ccgacgtcag gtcgtcccgc
    20881 tccaccgact tgggaagatg cagcgcgagc cggtcgacca cgtacttcac cagcggcgcg
    20941 tacttgctga gcgcgatctc cttctgccgc tctttccggt tcctggagcg cggcgagcgg
    21001 ttctgaaggg cgtgaaggac cgacgagaag gccatgggtg ctcccccgag ctaacggttc
    21061 ccgcgcgcgc gccgcgaggc cggatgggcc tcctcgtaat ggcggcggag cgcggttctc
    21121 atgagaatca ggccgaagcc gatcccgacg gcggcgacga ggacgaaggc gatcgcggaa
    21181 cgcaggatgg tcacgatcca ggacgcgtgc gtggcggcgc tcaccagccc cgagagcagg
    21241 gcggccgcga tggccgtgac cgcggtgagg cggacgagga gcgcgcgcac ggtcagacgc
    21301 cctccggcac ttcgtcgctg gtgaccaccg tgaacggccc cggctccgga gccgtctcct
    21361 tgggagccat gccgagcacg cggcgcgcga gctggtagac gcacgtcgcg gcgggcgagt
    21421 agggataggt cgagaggaaa ggctcctggc gcagcaccga cttcggcacc gattcgtcgg
    21481 ccaggatcgc gccccagtat tcgatgtcca ggttcaggaa ccgcttggcc acggagccga
    21541 cacgccgcgc gacggccgcg gcctcttcct ccgaggcggc ttcgttcacg atcagggcgg
    21601 ggacgcagcg gatgctgcgc cggctgagca ctttgatcat ggcgtaggcg tcggagaagg
    21661 cggtgggctc gggggtggtc acgaccagga ttggatcggc gacctgcgcc agggagaccg
    21721 cgttccggcc gatgcccgag gggcagtcga tcaggatcag gtccacgtcg cgctccagct
    21781 ccgcgaggga gcggatcagc acttcggtgc ggtactcgtc gagattcgcg agctcctcga
    21841 ccccgctcga ggcgggaagg atccggatgc cgtcggggcc ttcgagcacc acgtccgcga
    21901 cgctgcgcgt tccgaggatc acgtcctgaa ggtcgtactg gggcgcgatg ccgaggagca
    21961 gatccacgtt cgccaggccc aggtcgccgt cgacgacgag gacccggatg ccctgccgcg
    22021 cgagcgcgat cgccaggttc gtcacgatgt tcgtcttgcc gacgccgccc ttgccgctcg
    22081 tcaccgcgac gatgcggcgg cgcgggcggt tcgacggcgg gacgatcgcc ggcttccgtg
    22141 ccgcgcgggg cgtccgcgcc ttcttcgcgg gacgcttccg ccgctccacg ttcgattccg
    22201 ccttattctc gatctcggtc atggcgctcc gggttccctc gtgcgcgcac tcgtggagcg
    22261 cgcgccgttc gtgccgctcg tcgatcatcg gttggctcgt cggattcatc ggcccgtcgt
    22321 gcaactcgta tgccgcaggt ttaagtcgtt gcgttgcttc gccgcggcgc tccccttccc
    22381 cgtggagcgc cggtcggttt ggcggcagcc cccgatcgcc gcacaacttc tggccgctcg
    22441 ccgccgccgt gccggcgcgg ccgcgggcgg cccgtcccgg tctaggagcg cagccggcgc
    22501 tgccgcgcgg ctcgcgacgc gcggttgagc tcgtcttcga tctcctcgaa gacgtcctct
    22561 tccgtcgcgg cgccgggcgc acggcccaga tcggcgagcg cggcccagtg ctccttggtc
    22621 gaacccagca ggagccgccg tccatccact tccaccagca ccaacgtccg gccgattccc
    22681 acccgggtcc gcgacacgac gtggagcggc agaacctcct ccgcgtccgg cgagcccttg
    22741 gggcgcgcgg ccaggacgcg gctcgctccg tagagcaccg cggcggacac ggcgaacgcg
    22801 gtcgcgtaga tccagggcat gctcatcggt agctcctccg gtccgttccg gccatggcga
    22861 tgacgcgcac cgcggcccgc ccttccaggg tgatcagctc gccataggcg acggtggttc
    22921 cattcgcgac gacctcgacc ggcgagagcg cggcccgcgt gagcgggagc acgtcgccgg
    22981 cgcgaagggc gctcagctcc tcctgggtca ggtcggcgtg gcccgcgtcg accacgacgt
    23041 ggtagcgctc gcgcggcgcg ggcacgtacg gcgcttcccc gcggagggcg gcgatctctt
    23101 ccggcgtgag cagtcggctc atggcgttcc ggtcagcggg ccgcgctctt cgtggtctga
    23161 ccgaccacgg cgcggacgaa ggcgagatag cggcgcatcg cgagacgttc gaccgcgctc
    23221 atggcgcggc cgcgaacctg ggcggcgtgc gacttccggc tccgggactt cgaggcgcga
    23281 ggcatcgtcg ttctcccttt cgagaggctt cgcggatcgt ccgatccgcg cggtgactgg
    23341 ctggttacgg cgaggcaaaa ggcaacgggc gtgccgagcg gcgaaaggga atttgccggt
    23401 gctgtaaccg cctgtggcgc tgaggcttga aggattggag tgaactgcgg ggggagccgc
    23461 tcgacatcgg caggcgcggc gaaaacttga gcgaaatgcg agagacagtg cagaaaaacg
    23521 gccatgttcg gccggccgcc accgtcgctc gaaagcccga caagggtgcc ggccgatgtc
    23581 cggctggccg ccgtgaccga tcgcaagatc gatccgggct ccggccagcc gctcttctac
    23641 aagatcagca tcggccctgt tacaagatca gcatggcgtc cccatacgaa tagaaccgat
    23701 acccttcccg aacggcctct tcataagcac ggagcaccct gtccctcccc gccagcgccg
    23761 acaccaagaa cagcaagctc gacttcggca aatggaagtt cgtgaccatc ccatccacga
    23821 cgcggaacgc gaaccccggc aggacgaaca gggacgtctc gcgccacgcg ccgtcggacg
    23881 attcgccgtc cctcgcccag gactcgagcg cgcgcacggc ggtcgtcccc acggcgacga
    23941 ccctcccgcc cgcgcgccgc gcggcgcgaa gggcctccgc ggtctcgggg ggcacccggt
    24001 agggctcggg atcgagggcg tgctcggcgg gatcctcggt c
//
LOCUS       DASSLZ010000132         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1055758, whole genome shotgun sequence.
ACCESSION   DASSLZ010000132 DASSLZ010000000
VERSION     DASSLZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055758"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..326)
                     /locus_tag="VFM00_04995"
     CDS             complement(114..326)
                     /locus_tag="VFM00_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET7497637.1"
                     /translation="MNWDTVEGNWKEFKGRVREQWGRLTHDEVDVIGGRRDKLVGKLQ
                     EHYGIAREEAERQVREWTDMLEVSRP"
     gene            complement(615..1109)
                     /locus_tag="VFM00_05000"
     CDS             complement(615..1109)
                     /locus_tag="VFM00_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HET7497638.1"
                     /translation="MSKLVTAIFNSRAAAEMAVDELTRAGFAPEDVSLLMSESTRGRE
                     FAMKKGTKVPEGTATGATVGGVIGAVAAGLAAVGSLAIPGLALVAAGPVVAALAGLGA
                     GAAAGGLTGALIGLGLPEHEAKFYDKEIAKGGILLGVYAHDDRQKLAKDILEAAGAEK
                     VGRA"
     gene            1361..2416
                     /locus_tag="VFM00_05005"
     CDS             1361..2416
                     /locus_tag="VFM00_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7497639.1"
                     /translation="MKIAQVAPLHESVPPRLYGGTERVVSYLTEELVSLGHQVTLFAS
                     GDSVTAAELIPGSPTALRLNASAGDPIAAHVLLLEKVFQQAHRFDVVHFHCDYLHFPL
                     SRRSRLPNVTTLHGRLDLPDLEPLYREFANLPLVSISNAQRTPIPWAGWRATVYHGLP
                     ESAWTPRLDPGGYLAVLGRVSPEKGVDVAIEIARRAGVPLRIAAKVDRADQDYFDAVI
                     RPLLREPGVEFLGEIGEGDKEAFLGGAMALLFPIDWPEPFGLVMIEAMACGTPVIAYR
                     RGSVPEVLDDGATGYVVSDVAQAVTAVERLEAIDRSRVRRVFERRFSARRMARDYLDV
                     YAGIAQPEHPRAYADRS"
     gene            2400..4580
                     /locus_tag="VFM00_05010"
     CDS             2400..4580
                     /locus_tag="VFM00_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET7497640.1"
                     /translation="MPTEADDLIQIQDQFYILASSTRIDDRTHVLKHGDSMAVLDRFG
                     DVAPVGFGELGLYHDGTRFLSRLGLTIGWHRPLLLSSTVTRDNTRVIVDLTNPDLPIA
                     EGVVIPRGTLHVSREMLLWDGVCYERLRVANHGLATLTVPLGLTFDADFADIFEVRGT
                     KRAQRGRRLPPENESPAVLRFDYDGRDHVRRTTRIVCSTPADTASGGELRFDLHLEEH
                     SFWELLTTFAFETGRARPEPVAYDEALGCLARSLEQGCRGDCGIRSSNAFFNDWLRRS
                     LADIHMMTTDTPTGPYPYAGVPWFSTPFGRDGIITALQTLWVNPWIARGVLAYLAATQ
                     ADTVDEERDAQPGKILHEARGGEMAALGEVPFGRYYGSVDATPLFVVLAGAYYERTGD
                     RELAAALWPHVERALAWIEGDGDPDRDGFVEYARRSSTGLVQQGWKDSYDSVFHADGS
                     LAEPPIALCEVQGYVFAAYHAAATLAGVLGDESESARLVMRAEALRARFEEAFWCEEI
                     ASYALALDGAKKPCRVRTSNPGHCLYTGIVSKERAARMADTLMSEDFFTGWGLRTVST
                     REARYNPMSYHDGSVWPHDNAIVAAGLSRYGFRHAASRLLGGLFDATLHFELHRTPEL
                     FCGFARRSGAGPILYPVACAPQSWSAGAPFLFLQACLGLTVDAPRRTITLHRPELPES
                     VEQITLGELVVGDAQVELSFQSHEHGVDVNVMRKRGEVEVIVIK"
BASE COUNT          692 a         1714 c         1602 g          829 t
ORIGIN      
        1 ggcccttcca tctatgggaa tgggttgggt ggggaaccgc ccgattcccg tgggaaggcc
       61 aattggagga ggagggcccc ccgcttgggc ttctacgagg attttattgc ggtctaaggg
      121 cgcgagacct cgagcatgtc ggtccattca cggacctgac gctcggcttc ttcgcgcgcg
      181 attccatagt gctcctggag cttgccgacg agcttgtccc ggcgtccgcc gatgacgtcg
      241 acttcgtcgt gcgtgagacg gccccactgt tcccggacgc gtcccttgaa ttccttccag
      301 ttgccttcaa cggtgtccca gttcatgtca tgcctcgctg gagctgatcg cttgaaatgt
      361 gcaccaggtg agcccgcacg gaacgtcctg ctggctcaac ggcacccgtg attgctggaa
      421 tgtcgtttca ggtatctgga gccgggtcga gttgccttcg ctccgtgaac tcgtcggatg
      481 gctgacttag cagcctccgt gccgattcca gactccgtgt aactcattgg gaacagaggg
      541 ggagcgcgtg cagccgctcc ccgtccccgt gtaactcctg gaaaacccgc tgtaccgtgt
      601 acaaatggga tcgctcaggc gcggccgacc ttctcggcac cggcggcttc caggatgtcc
      661 ttcgccagct tctgccgatc atcgtgggca tagacgccca gcaggatgcc gcccttcgcg
      721 atctccttgt cgtagaactt ggcttcgtgc tcggggagac cgagcccgat caaagcaccc
      781 gtcaatccgc cggctgcggc cccggctccg agcccggcca gcgcggcgac gaccggtccg
      841 gcagcaacga gcgcgagacc ggggatggcg agcgatccga ccgcggcgag tcccgccgcg
      901 accgcgccga tgacgcctcc gaccgtggcc ccggtcgccg ttccctccgg aaccttcgtc
      961 cccttcttca tggcgaactc acgtccgcgg gtggactccg acatgagcag gctgacgtcc
     1021 tccggcgcga acccggcgcg ggtcagctca tccacggcca tctcggccgc ggctctcgaa
     1081 ttgaagatgg ccgtcacgag tttcgacatg gcttcctcct ggctcgattg aaagaaagga
     1141 cgcgctcgtt gttcgctgct gccgcgttgt gggtgcggcc catgtacggg ccggctcgat
     1201 cgcagggttc gtgccacgcc gaggccgcgc cggcaacgca tggagagcgc ccatccgaac
     1261 cgcccggccg ggcatgtgtt ctgctaaacg ggggtcgggc cggtcacgtc acccattgcc
     1321 ctgctccaat gctcctccga gcgcggaggt ccgtcctttc atgaagatcg cccaggtcgc
     1381 tccactccac gagtcggtcc cgccccggct ctacggcgga acggagcgcg tcgtctcgta
     1441 tctcaccgag gagctcgttt ccctcggaca ccaggtcacg ctgttcgcaa gcggcgattc
     1501 cgtaaccgcc gccgagctga tccccggatc gcccacggcg ctccggctga acgcctccgc
     1561 cggcgatccg atcgccgcgc acgtgctgct gctcgaaaag gtgtttcagc aggcgcaccg
     1621 cttcgacgtg gttcacttcc actgcgacta ccttcacttc ccgctctcgc gccggagccg
     1681 ccttccgaac gtcacgacgc tgcacggccg gctggatctg ccggacctgg agccgctcta
     1741 ccgcgaattc gcgaaccttc cgctcgtctc gatctcgaac gcccagcgaa cgccgatccc
     1801 gtgggccggg tggcgggcga cggtctatca tggactgccg gagtcggcat ggacgccgcg
     1861 gctcgacccg ggcggctatc tcgccgtgct ggggcgcgtc tccccggaga aaggggtcga
     1921 cgtcgcgatc gaaatcgcgc ggcgcgccgg ggtcccgctg cgcatcgcgg cgaaggtgga
     1981 ccgcgccgac caggactact tcgacgcggt gatccgcccg ctcctgcgtg agccgggcgt
     2041 ggagttcctg ggtgagatcg gcgaagggga caaggaagcg ttcctgggcg gcgcgatggc
     2101 gctcctcttc cccatcgact ggcccgagcc gttcgggctg gtcatgatcg aggccatggc
     2161 gtgcggcacg ccggtcatcg cttaccggcg cggatcggtc ccggaagtgc tcgacgacgg
     2221 agcgacgggg tatgtggtgt ccgacgtggc gcaggccgtg acggccgtcg agcggctcga
     2281 ggcgatcgac cgctcgcgcg tgcggcgcgt ttttgagcgc cgcttctcgg cgcgccgcat
     2341 ggcgcgggac tacctcgatg tgtacgccgg aatcgcacaa ccggagcacc cgagagccta
     2401 tgccgaccga agctgacgat ctcatccaga tccaggacca gttctacatc ctggccagct
     2461 cgacgcgcat cgacgaccgc acgcacgtcc tgaagcacgg ggactcgatg gcggtcctgg
     2521 accgcttcgg cgacgtggcg ccggtgggct tcggcgagct gggcctctat cacgacggca
     2581 cccgcttcct ctcccggctg gggctcacga tcggctggca tcgtccgctc ctgctctcct
     2641 ccacggtgac gcgggacaac acccgggtga tcgtggacct cacgaatccc gatctcccca
     2701 tcgccgaggg ggtcgtgatc ccgcgcggca cgctgcacgt gtcgcgggag atgctcctct
     2761 gggacggcgt ctgctacgag cggctccgcg tcgcgaacca cggcctggcc acgctgacgg
     2821 tgccgctggg cctcaccttc gacgccgact tcgccgacat tttcgaggtc cgcggcacca
     2881 agcgcgccca gcggggacgg cgcctgcccc cggagaacga gtcgcccgcc gtgctgcgct
     2941 tcgactacga cgggcgggat cacgtgcggc gcacgacgcg gatcgtctgc tccacgccgg
     3001 cggatacggc gtcgggaggt gaacttcgct tcgatctcca cctggaggag cactcgttct
     3061 gggagctcct caccacgttc gctttcgaga ccgggcgggc gcgtccggag ccggtggcct
     3121 acgacgaggc gctcgggtgc ctggcgcgct cgttggagca gggctgccgc ggcgactgcg
     3181 gcatccggtc gtccaacgcc ttcttcaacg actggctccg ccgctccctg gccgacatcc
     3241 acatgatgac caccgacacg ccgaccggcc cctaccccta cgcgggcgtg ccctggttca
     3301 gcactccctt cggtcgcgac gggatcatca cggccctgca gacgctctgg gtcaacccct
     3361 ggatcgcgcg cggcgtgctc gcctatctcg cggccacgca ggccgacacc gtggacgagg
     3421 agcgggacgc ccagccgggc aagatcctcc acgaggcgcg cggcggcgag atggcggccc
     3481 tgggcgaggt gcccttcggc cgctactacg ggagcgtcga cgccacgccg ctgttcgtgg
     3541 tgctggccgg cgcctattac gagcgcacgg gcgaccgcga gctggccgcg gcgctatggc
     3601 cccacgtcga gcgcgcgctc gcgtggatcg aaggggacgg cgatcccgac cgggacggtt
     3661 tcgtcgagta cgcgcgccgc tcgtccacgg gactggtgca gcaggggtgg aaggactcct
     3721 acgactcggt cttccacgcg gacggctcgc tcgccgagcc gccgatcgcg ctctgcgaag
     3781 tacagggcta cgtgttcgct gcctaccacg cggcggccac cctggccggc gtcctgggcg
     3841 acgagagcga gtcggcgcgg ctggtcatgc gcgccgaggc tctgcgagcg cgcttcgaag
     3901 aggccttctg gtgcgaggaa atcgcctcgt acgcgctcgc gctcgacggc gccaagaagc
     3961 cctgccgcgt gcgcacgtcg aacccgggcc actgtctcta cacgggcatc gtctccaagg
     4021 agcgcgccgc gcgaatggcc gacacgctca tgagcgagga cttcttcacg ggatgggggc
     4081 tgcgcaccgt ctcgacgcgc gaagcccggt acaacccgat gtcctaccac gacggatcgg
     4141 tctggcccca cgacaacgcg atcgtcgcgg ccgggctctc gcggtatggg ttccgccacg
     4201 cggcgtcccg cctgctcggg gggctcttcg acgccaccct ccacttcgag cttcaccgca
     4261 ccccggagct cttctgcggc ttcgcgcgcc ggtcgggggc gggccccatt ctctacccgg
     4321 tcgcctgcgc tccccagtcc tggtccgccg gcgcgccctt cctgttcctg caggcctgcc
     4381 tcggcctcac cgtcgacgcc ccgcgccgca ccatcacgct gcaccgcccc gagctgcccg
     4441 agagcgtgga gcagatcacg ctcggcgagc tggtcgtcgg cgacgcccag gtcgagcttt
     4501 cgttccaatc tcatgagcac ggggtcgatg tgaacgtcat gcgcaaacgg ggcgaagtcg
     4561 aggtcatcgt catcaagtag ctagcgtcgc tgaaccctgc ctgtgcgcgc ggcgtctcga
     4621 cgggcggccg ggcgcactgc gttttggcgc gaggggaggc aacgctccgg gcgcttgtcc
     4681 tcgcccggcg ttgcggcctg ccgacggcat cgagatggcg gggcgatggt cgcttcgctc
     4741 cctcgctccg actacctcaa gttcgggtcc ggtcgctgtg tcctcatggc tccctcatgt
     4801 cacgggatac ggggccgcag catggaagag atcacgc
//
LOCUS       DASSLZ010000133        12187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_932949, whole genome shotgun sequence.
ACCESSION   DASSLZ010000133 DASSLZ010000000
VERSION     DASSLZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12187
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932949"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..970)
                     /locus_tag="VFM00_05015"
     CDS             complement(92..970)
                     /locus_tag="VFM00_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7497641.1"
                     /translation="MSRADVALLFICAIWGTTFALLRDALHAVSPAELVALRFTIAAV
                     VLALIYFRRLRGWRPEWIQDGLTIGIVVAVGYITQVIGLRTVTASRSAFITSMSIAFV
                     PFVAFAALRTRPVMGESLGVLLAVAGLVLFSADAGFSLQKGELWTLACAAAFAVQIVV
                     THVAGKRSPAVPISVLQTVVGAAAGWTLVLAGGGATTPARAIPWGVLLYLGIVATAFI
                     IVVQTWALARTSSVKAGVLYATEPIFAAIFAVSFFGERMTPRELAGAALILAGVFVSE
                     LWRPLRARFAARATSA"
     gene            complement(984..1652)
                     /locus_tag="VFM00_05020"
     CDS             complement(984..1652)
                     /locus_tag="VFM00_05020"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET7497642.1"
                     /translation="MDPRIPTSTVRRLSAYYRVLEALEIEGQRTVSSQGLARESGVTS
                     AQVRKDLSYFGNFGKRGLGYGVARLKKEIRLILGLNRRWRVALVGAGNMGSALFSYKE
                     FRRQGFDFVGVFDVSADRVGQDWRDLIVRHIDDLETETARLGIEIGIIAVPARAAQMV
                     ADRMVAAGMRGVLNFAHRRIQVPPTVALRTVNLSIELESLAFEIKALNMKGTRTRRRR
                     VAAG"
     gene            complement(1654..4092)
                     /gene="gyrA"
                     /locus_tag="VFM00_05025"
     CDS             complement(1654..4092)
                     /gene="gyrA"
                     /locus_tag="VFM00_05025"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET7497643.1"
                     /translation="MATNEKIIPIYVEDEMRNSYIDYSMSVIVSRALPDVRDGLKPVH
                     RRILVAMRELNLLHDRPFRKSAKITGDVTGNYHPHGTAAVYETMVRLAQSFSMRYPLV
                     DGQGNFGSVDGDAPAAERYTEARLTEFAEEMLRDIEKDTVDMRPNYDESREEPVVLPS
                     AVPNLLVNGSSGIAVGMATNVPPHNLREIVDATIHVIDNPDCPIEDLLRIVQGPDFPT
                     AGIIYGRQGILDCYTTGRGHITVRSNATIEEYKKDREAIIVTEIPYMVSKAALLEKIA
                     DLVKDGTLDGISDLRDESDREGMRIVIELKRDAQPRVILNQLFKHTQMQTTFGANMLA
                     LVGNRPQTMTLKEMIQHYVAHRQEVVVRRTRFDLNEAEKRAHILEGFKIALDHIDEIV
                     ALIKASANVEAARQGLMSTFGLSEIQASAILEMRLSRLTGLERKKIEDEYLEVIQLID
                     RLRAILESPALVLGIIKEELAGIRERFGDDRRTRIESASGEFEVEDLIAEEDMVITIS
                     HAGYIKRLPVTTYRSQRRGGRGVTGAGTKEEDFIQHLFIASTHSYILVFTDKGRVHWL
                     KVHEIPQAGRTAKGKAVVNLVEMSQQERVAAVLPVKQFDDKHFILMTTARGIVKKTPL
                     SAYSNPRRGGIVAIGVDGEDKLIDAVLTDGTQEIILSKRDGKAIRFQETDVRPMGRTA
                     RGVRGVTIEGDDAVVGVIAVRREASLLVATENGYGKRSPISEYRITGRGGKGIISIQA
                     NDRNGRVVAALEVVPDDEVMLITRGGMVIRTQVAGISEIGRNTQGVRLINLEPGDQLI
                     DVAKVEESAEGEEA"
     gene            complement(4131..6086)
                     /gene="gyrB"
                     /locus_tag="VFM00_05030"
     CDS             complement(4131..6086)
                     /gene="gyrB"
                     /locus_tag="VFM00_05030"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET7497644.1"
                     /translation="MAQPPDPSNTPKSAAPPDGDGHSYDARNIQVLKGLEGVRKRPAM
                     YIGSTGQTGLHHLVYEVVDNSVDEALAGFCANVQVTIHSDSSVTVVDDGRGIPVDVHP
                     AQGRPALEVVMTTLHAGGKFDESSYKVSGGLHGVGVSVVNALSDWLEVEVRRDGKRYH
                     QRYEHGIVKTELAELGPTEETGTTVTWRPDANIFETLDYNFDTLSQRLRELAFLNKGI
                     RIQFTDERTGKKHDFQYEGGIASFVKYLNTNKTVLHPEPIYHQRERDRMAVEIAIQYN
                     DGYVENVFSFVNNINTVEGGTHLIGFRTALTSTINSYAEREGLTKGLKDITLGGDDVR
                     EGLTAVVSVKLHDPQFEGQTKAKLGTTEAKGVVQSVVGEGLRNYFEENPGVARKIVDK
                     CIQTARAREAARKARDLARRKSILDGGSLPGKLADCQSTNPAECEIYLVEGDSAGGSA
                     KMGRDRKYQAILPLKGKILNVEKASLDKMLSNEEIRTIITALGTGIDDDFDADKARYH
                     KIIIMTDADVDGAHIRTLLLTFFFRHMRPLIERGYIYIAMPPLYRIAKGKDVRYAYND
                     AEREKALEEIGRKGIHMQRYKGLGEMNPDQLWSTTMDPEFRSVSKVTLDDLVEADQIF
                     TILMGDQVEPRRKFIEEYADSVKNLDI"
     gene            complement(6100..6456)
                     /locus_tag="VFM00_05035"
     CDS             complement(6100..6456)
                     /locus_tag="VFM00_05035"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HET7497645.1"
                     /translation="MKPDRPPRRGAGEMEAVGGFLDDVLAGIRMESRFQGAQATDEWT
                     AVVGPEVARRTRPVGVRDGELLVEVQGAVWMGHLAVLRQGVLEALNRRLPDSARLKSI
                     RLVPMRFKEERIESEE"
     gene            complement(6453..7556)
                     /gene="recF"
                     /locus_tag="VFM00_05040"
     CDS             complement(6453..7556)
                     /gene="recF"
                     /locus_tag="VFM00_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HET7497646.1"
                     /translation="MRLQSLELRDFRNHREARTVVGDGSALILGPNGSGKTNWIEAAV
                     LLSLGRSFRGARDRELIRSGADAFEIRGVVAGRGGVRRELLARGSREGVREVRVDGEA
                     LPRLTDLLGRFPTVHFSVDDVSVFGGGVPERRRFLDVALCQLEPAYVGSLREYTTALK
                     QRNRLLMYRGRGVAAAAEAADPEIGVWEAILARAGAELDRRRAALAADLHAGMRALSA
                     RVDPSLNPVLEYVSAGAGTPDERAARLEAMRIRDARLGWTAEGPHRARLVCRIGGMEL
                     AEGASRGYSRLYAILLRLALARVLEERLDEAPVVLLDDPESELDARWIGPILALIPES
                     SQMIVTACRALTVAPDRVASLPIETLAACAGAA"
     gene            complement(7561..8697)
                     /gene="dnaN"
                     /locus_tag="VFM00_05045"
     CDS             complement(7561..8697)
                     /gene="dnaN"
                     /locus_tag="VFM00_05045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET7497647.1"
                     /translation="MKCSIAQTELLQALSLISSAVPSRTTLPVLANILVEATDEGLQM
                     TATDLDLSVTTRAMADVKAEAALTVPAKKLLELVRKLPKEELKLEAKDLTLNVVSKSG
                     RFKFLCIRPEEFPARVNVAPDTALTIDAKQLERMIKRTIYAVSTDETRPALNGALLQV
                     AEGEIRLVATDGHRLARATLKHPGAVKGALKGDVIVPLKALTHLHRLVAESADPVHVE
                     LSKNHARFTVGKTSLTTKLIEGPFPNYEQVLPKNNNKHLHVKTVDLASSLDRVSVFSD
                     SLTRQVKFAVTRNKLTLIVQTPDQGEANESLDVRYDAEDLEIGYNAGYLLDILRTMDS
                     EEVTVQLNTPVTAGLLVPGAPTDAKGTPALEGLLCLVMPLRLAG"
     gene            complement(9182..10627)
                     /gene="dnaA"
                     /locus_tag="VFM00_05050"
     CDS             complement(9182..10627)
                     /gene="dnaA"
                     /locus_tag="VFM00_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET7497648.1"
                     /translation="MIEKAAVDLWERALGSIQDSTSPQTYETWFRSLRPVSLNGSLVL
                     EVPSQFYVDWLDQHYRPLIETSVASAAGATIPVTFQVRQEDASDAPPWGALPSEPNGS
                     SGAGQAFTKEDFIGPGGPGGREESRPARTSGWSGGFPSRDESALVPQNTFDTFVVGNG
                     NQFAHAVCQAVAQSPGERYNPLFLYGGVGLGKTHLMHAIGHFVRAQRPHAKVFFVSAE
                     KFMNEMIYAIQHATTLEFKARYRSADLLLIDDIQFLEGKESTQEEFFHTFNALHDQHR
                     QIVLTSDGPPNSMTALEERLLSRFTWGVVADIQPPDLETRVAIVKKKAELQNRMVPNE
                     IALLLASNIKNNIRDLEGSLARLLAFAELTNSKLTVEFAQDVLRDQIKPDLVRFDVHD
                     IQRIVSRHFNVTEESLRGKRRTDTIAFPRQIGMYIARTLTDLSLAEIGAKFGGRDHTT
                     VLHACQKIEALISNDPEMRGVVEELMAQLTA"
     gene            11029..11163
                     /gene="rpmH"
                     /locus_tag="VFM00_05055"
     CDS             11029..11163
                     /gene="rpmH"
                     /locus_tag="VFM00_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET7497649.1"
                     /translation="MKRTFQPHNRRRKKVHGFRERMSTRGGRAVLKRRRDRGRKRLTV
                     "
     gene            11189..11536
                     /gene="rnpA"
                     /locus_tag="VFM00_05060"
     CDS             11189..11536
                     /gene="rnpA"
                     /locus_tag="VFM00_05060"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET7497650.1"
                     /translation="MAGGVPTRERRLRSGWQFREVYRAGSSYHGTLMSLVSLGKPEDH
                     GRWAFVASRKVGPAVRRNRAKRLLRESFRRLPEDRRNEPRWRVWIARAACSRSDLHHV
                     EAEMARLVKQERR"
     gene            11533..11781
                     /gene="yidD"
                     /locus_tag="VFM00_05065"
     CDS             11533..11781
                     /gene="yidD"
                     /locus_tag="VFM00_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET7497651.1"
                     /translation="MTRAAAAIRSGFGLRALAILLVRAYRFAIAPVLPKSCRFTPSCS
                     AYAEEALHKHGLFRGLWLAAKRILRCHPFHPGGYDPVP"
     gene            11798..>12187
                     /locus_tag="VFM00_05070"
     CDS             11798..>12187
                     /locus_tag="VFM00_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497652.1"
                     /translation="MDRRTLLAVVLIMAVLLVDQVIWSRWTKSRQPVVPPTAGGSAVP
                     GDSATARAALADSALHGVPASQPAEGASSAARVGAPAVTGGGPLISARVAGAPVTEDS
                     LATGQFRARFTSEGGAVSSWVLPGYRDP"
BASE COUNT         1827 a         4113 c         4122 g         2125 t
ORIGIN      
        1 accgagatgg gatatcccga gcctccgccc gtggcgaagg ccgcggagtc cgcgttcctc
       61 gagctcgccc gcgcgctgcc gggcgctccg gctacgccga agtagcgcgc gccgcgaagc
      121 gcgcgcggag cggccgccag agctcgctca cgaagacgcc ggccaggatg agcgcggccc
      181 cggccagctc ccgcggcgtc atccgctcgc cgaagaacga gacggcgaag atcgcggcga
      241 agatcggctc ggtcgcgtag aggacgccgg ccttgaccga agaggtccgg gccagcgccc
      301 acgtctggac cacgatgatg aaggcggtcg cgacgatgcc cagatagagc aggacgcccc
      361 aggggatcgc gcgcgccggc gtcgtcgccc cgcctccggc cagcaccagc gtccatcccg
      421 ccgccgcgcc caccacggtc tggagcacgc tgatcggcac ggccgggctg cgcttccccg
      481 ccacgtgggt cacgacgatc tgcaccgcga aggcggccgc gcacgccaac gtccagagct
      541 cccccttctg gagcgaaaag cccgcgtcgg ccgagaagag cacgaggccg gccaccgcga
      601 gcaggacgcc gagcgattcc cccatcacgg gccgcgtgcg gagtgcggcg aatgcgacga
      661 agggcacgaa cgcgatgctc atcgaggtga tgaacgccga ccgcgaagcg gtcacggtgc
      721 gaagcccgat gacctgggtg atgtagccga cggcgaccac gatgccgatg gtgaggccgt
      781 cctggatcca ctccgggcgc cagccgcgca gcctccggaa gtagatcagc gcgaggacca
      841 cggcggcgat ggtgaagcgg agcgcgacga gctcggccgg ggagaccgcg tgcagggcgt
      901 cgcgcaggag ggcgaaggtc gtcccccaga tggcgcagat gaacaggagg gcgacgtcgg
      961 cgcggctcaa cgcggccgga ccgtcacccc gcggcgacgc ggcggcggcg cgtccgcgtc
     1021 cccttcatgt tcagggcctt gatctcgaac gcgagacttt ccagctcgat ggagaggttc
     1081 acggtgcgga gggcgacggt cggaggaacc tggatccgcc ggtgcgcgaa gttgaggacg
     1141 ccgcgcatgc ccgccgcgac catccggtcg gccaccatct gcgcggcgcg cgcggggacc
     1201 gcgatgatcc cgatctcgat gccgaggcgg gccgtttcgg tttcgaggtc gtcgatgtgc
     1261 cggacgatca gatcgcgcca gtcctgcccg acgcggtcgg cgctcacgtc gaacacgccg
     1321 acgaagtcga agccctgccg gcggaattcc ttgtaggaga agagcgccga tcccatgttc
     1381 ccggcgccga ccagggcgac ccgccagcgg cggttcagcc ccaggatgag gcggatctcc
     1441 ttcttcaggc gcgcgacgcc gtagcccagg ccgcgctttc cgaagttgcc gaagtaggag
     1501 aggtccttcc ggacctgggc cgaggtgacg ccgctctcgc gggcgagccc ctgggaggag
     1561 acggtccgct gcccttcgat ctccagcgct tcgaggacac ggtaatacgc cgagagccga
     1621 cggacggtcg acgtcggaat gcgtgggtcc agcctacgcc tcctcgccct cggccgactc
     1681 ttccaccttg gcgacgtcga tcagctggtc gcccggctcc aggttgatga gccgcacccc
     1741 ctgcgtgttc cgcccgatct cggagatgcc ggccacctgc gtgcggatga ccatgccgcc
     1801 gcgcgtgatc agcatcacct cgtcgtcggg caccacttcc agcgccgcca cgacgcggcc
     1861 gttccggtcg ttggcctgga tcgagatgat ccccttgccg ccgcgcccgg tgatgcggta
     1921 ctcgctgatc ggcgaccgct tgccgtagcc attctccgtg gcgaccagga gcgacgcctc
     1981 gcggcgcacc gcgatgacgc ccaccaccgc gtcgtcgccc tcgatcgtca cgccccgcac
     2041 gccacgcgcg gtgcgaccca tcgggcgcac gtcggtctcc tggaagcgga tcgccttgcc
     2101 gtcccgcttg gagaggatga tctcctgcgt cccgtcggtg agcaccgcgt cgatcagctt
     2161 gtcctcgccg tccaccccga tcgccacgat gccgccgcgg cggggattgg aataggccga
     2221 gagcggcgtc ttctttacga tgccgcgcgc ggtggtcatg agaatgaagt gcttgtcgtc
     2281 gaactgcttc accggaagca ccgccgccac gcgctcctgc tgggacatct ccaccaggtt
     2341 cacgacggcc ttccccttcg cggtgcggcc ggcctgcggg atctcgtgca ccttgagcca
     2401 gtgaacgcgc cccttgtcgg tgaagaccag gatgtagctg tgcgtcgagg cgatgaagag
     2461 gtgctggatg aaatcctctt ctttggtgcc ggcgccggtc acgccgcgtc cgccgcgccg
     2521 ctggctccgg taggtcgtca cgggaagccg cttgatgtag cccgcgtggc tgatcgtgat
     2581 gaccatgtcc tcttcggcga tcaggtcctc gacctcgaac tcgccggagg cggactcgat
     2641 ccgggtgcgg cgatcgtcgc cgaagcgctc gcggatcccc gcgagctcct ccttgatgat
     2701 cccgagcacg agcgcggggc tctcgaggat ggcccggagc cggtcgatca gctggatcac
     2761 ctcgaggtac tcgtcctcga tcttcttccg ctccagcccg gtcagccgcg agagccgcat
     2821 ctcgaggatg gcgctcgcct ggatctccga cagcccgaag gtgctcatca gcccctggcg
     2881 cgccgcctcg acgttggccg aggccttgat cagcgccacg atctcgtcga tgtggtcgag
     2941 ggcgatcttg aagccctcga ggatgtgcgc ccgcttctcc gcctcgttca ggtcgaagcg
     3001 cgtgcggcgc accacgactt cctgccggtg cgccacgtag tgctggatca tctccttcag
     3061 cgtcatcgtc tgcggccggt tcccgaccag cgcgagcatg ttggcgccga aggtggtctg
     3121 catctgcgtg tgcttgaaga gctggttcaa gatgacgcgc ggctgcgcgt cgcgcttcag
     3181 ctcgatcacg atccgcatcc cctcgcgatc cgactcgtcg cgcaggtcgg agatgccgtc
     3241 cagggtcccg tccttgacga ggtcggcgat cttttccagg agcgccgcct tgctcaccat
     3301 gtacgggatc tcggtgacga tgatcgcctc gcggtccttc ttgtactcct cgatcgtcgc
     3361 gttggagcgg accgtgatgt gcccgcgacc cgtcgtgtag cagtcgagga tcccctgccg
     3421 gccgtagatg atcccggccg tcgggaaatc agggccctgc acgatccgga gcagatcctc
     3481 gatcgggcag tcggggttgt cgatgacgtg gatcgtggcg tccacgatct cgcgcaggtt
     3541 gtgcggcggc acgttggtcg ccatgccgac cgcgatcccc gaggacccgt tcacgagcag
     3601 gttcgggacg gccgacggaa ggacgacggg ctcctcgcgg gactcgtcgt agttcggccg
     3661 catgtccacc gtgtccttct cgatgtcgcg gagcatctcc tccgcgaact cggtcaggcg
     3721 cgcctcggtg taccgctcgg ccgccggagc gtccccgtcc accgagccga agttcccctg
     3781 gccgtccacc agcgggtagc gcatggagaa cgactgcgcc aggcggacca tcgtctcgta
     3841 gacggccgcg gtgccgtgcg gatgatagtt gccggtcacg tcgccggtga tcttggcgga
     3901 cttgcggaag ggacggtcgt ggaggaggtt cagctcgcgc atcgccacca ggatccggcg
     3961 atggacgggc ttcaacccgt cgcgcacgtc gggcagcgca cgggacacga tgaccgacat
     4021 cgagtagtcg atgtaggaat tccgcatctc gtcttcgacg tagatcggaa tgatcttctc
     4081 gttcgtggcc atgcgttacg gttctccctg ctcgaggatc ggcaagcgga tcagatgtcc
     4141 aggttcttca cgctgtccgc gtattcttcg atgaacttgc gccggggctc gacctggtcg
     4201 cccatcagga tggtgaagat ctgatcggct tccaccaggt cgtcgagcgt caccttggag
     4261 acgctccgga actcggggtc catcgtcgtc gaccagagct ggtcggggtt catctcgccg
     4321 agccccttgt agcgctgcat gtggatgccc ttccgcccga tctcctccag cgccttctcg
     4381 cgctccgcgt cgttgtaggc gtagcgcacg tccttgccct tggcgatccg gtagagcggc
     4441 ggcatggcga tgtagatgta gccgcgctcg atcagcggcc gcatgtggcg gaagaagaac
     4501 gtcaggagca gcgtccggat gtgggcgccg tccacgtcgg cgtcggtcat gatgatgatc
     4561 ttgtgatagc gcgccttgtc ggcgtcgaag tcgtcgtcga tcccggtgcc gagcgcggtg
     4621 atgatggtcc ggatctcctc gttggagagc atcttgtcca gcgaggcctt ctccacgttc
     4681 aggatcttgc ccttcagggg caaaatcgcc tggtacttgc ggtcgcggcc catcttggcc
     4741 gagccgccgg ccgagtctcc ctcgaccagg tagatctcgc attcggcggg gttggtggac
     4801 tggcagtcgg ccagctttcc ggggagcgag cccccgtcca ggatgctctt ccggcgcgcg
     4861 agatcgcgcg ccttgcgcgc cgcttcgcgc gcgcgggcgg tctggatgca cttgtcgacg
     4921 atcttccgcg cgacgccggg attctcctcg aagtagttcc ggagcccttc tccgacgacc
     4981 gactggacca cgcccttggc ctcggtcgtc ccgagcttgg ccttggtctg tccctcgaac
     5041 tggggatcgt ggagcttcac gctcacgacg gcggtcagcc cctcgcgcac atcgtcgccc
     5101 ccgagcgtga tgtccttgag ccccttggtg agcccctcgc gctcggcgta gctgttgatg
     5161 gtgctggtga gcgcggtgcg gaacccgatc aggtgcgtgc cgccctcgac ggtgttgatg
     5221 ttgttcacga agctgaagac gttctcgacg tagccgtcgt tgtactggat ggcgatctcg
     5281 accgccatcc ggtcgcgctc gcgctggtga tagatcggct cggggtggag caccgtcttg
     5341 ttcgtgttca ggtacttcac gaacgacgcg atccccccct cgtactggaa gtcgtgcttc
     5401 ttcccggtcc gctcgtcggt gaactggatg cggatgccct tgttcaggaa cgcgagctcg
     5461 cgcaggcgct gggagagggt gtcaaagttg tagtccagcg tctcgaagat gttcgcgtcg
     5521 ggccgccagg tgaccgtggt tccggtctcc tcggtaggtc ccagctccgc gagctcggtc
     5581 ttcacgatgc cgtgctcgta gcgctggtgg tagcgcttgc cgtcgcggcg cacctcgacc
     5641 tcgagccagt cggagagcgc gttcacgacc gagacgccga cgccgtgcag cccgcccgag
     5701 accttgtagc tggactcgtc gaacttgccg ccggcgtgca gcgtggtcat gaccacttcg
     5761 agcgcggggc gtccctgcgc cggatggacg tccaccggga tgccgcggcc gtcgtccacc
     5821 accgtgacac tcgaatcgga atggatcgtg acctgcacgt tggcgcagaa gccggcgagc
     5881 gcctcgtcca cggagttgtc gaccacttcg tagaccaggt ggtgcagccc ggtctggccg
     5941 gtcgagccga tgtacatggc cggacgcttg cggacgccct ccagcccttt caggacctgg
     6001 atgttccgcg cgtcgtagga atggccgtcg ccgtcgggcg gggccgcgga cttcggtgta
     6061 ttcgagggat cggggggctg ggccaagctc aacgcctcct cactcttcgg actcaattcg
     6121 ttcctccttg aaacgcatcg ggacgagccg gatggacttg agtcgggccg agtcggggag
     6181 gcgccggttc agcgcttcca ggactccctg gcggagcacc gccaggtggc ccatccaaac
     6241 ggcaccctgc acttccacga gaagctcacc gtcgcgcacc cccacggggc gggtgcgccg
     6301 cgcgacctcg ggaccgacca ccgccgtcca ctcatcggtc gcctgcgcgc cctggaagcg
     6361 gctctccatg cggatcccgg cgagcacgtc gtccaggaac ccgccgaccg cctccatctc
     6421 gccggcgccg cggcgcggcg gacggtcggg cttcatgccg cccccgcgca ggccgcgagg
     6481 gtttcgatgg gcagcgacgc gacgcggtcg ggggcgaccg tcagcgcgcg gcatgcggtg
     6541 acgatcatct ggctcgactc cgggatcagc gccaggatcg gtccgatcca tcgcgcatcc
     6601 agctcggatt cggggtcgtc gagcaggacc accggggctt cgtcgagccg ctcttccagg
     6661 acccgcgcca gcgccagccg gagcaggatc gcgtagaggc gcgagtagcc gcgcgaggcc
     6721 ccttcggcca gctccatccc tccgatccgg cagacgagac gcgcccggtg cggcccttcg
     6781 gccgtccacc ccagccgcgc gtcgcggatg cgcatcgcct cgagccgcgc ggcgcgctcg
     6841 tcgggcgttc cggcgccggc gctcacgtat tcgagcaccg ggttcaggct cgggtccacg
     6901 cgagccgaca gggcgcgcat gccggcgtgc agatcggccg cgagcgcggc acggcggcga
     6961 tcgagctccg cgcctgcgcg tgcgagaatg gcttcccaaa ccccgatttc gggatcggcg
     7021 gcttccgcgg ccgcggcgac gccgcgtccg cgatacatca gaagccgatt gcgctgtttc
     7081 agcgcggtcg tgtactcgcg gagcgagccg acgtaggcgg gctcgagctg gcagagcgcc
     7141 acgtcgagga agcggcgccg ttcggggacc ccgccgccga acacggagac gtcgtccacg
     7201 gagaagtgga cggtggggaa acgcccgagc aggtcggtaa gccgcggcag cgcttctccg
     7261 tccacccgca cctcccgcac cccctcccgc gacccgcgcg cgagcagctc gcgccgcacg
     7321 ccgccccgtc ccgcgaccac gccgcggatc tcgaacgcgt ccgcgccgct tcggatcagc
     7381 tcccggtccc gcgccccgcg aaaggagcgg ccgagcgaca ggagaacggc ggcctcgatc
     7441 cagttcgtct tcccggagcc gttcggcccc aggatcaagg cgcttccgtc gccgacgacg
     7501 gttctggcct cacggtgatt tcggaaatcg cgcagctcga ggctttgtag tcgcacgcgc
     7561 tcagccggcc agccggaggg gcatgaccag gcagaggagc ccttccaggg ccggagttcc
     7621 cttcgcgtcc gtgggcgccc ccggcaccag gagaccggcc gtgacgggcg tgttcagctg
     7681 caccgtcact tcctcggaat ccatggtccg gaggatatcg agcagatagc cggcgttgta
     7741 gccgatctcg agatcctcgg cgtcgtagcg gacgtcgagc gattcgttcg cctcgccctg
     7801 atcgggcgtc tggacgatga gcgtcagctt gttccgcgtc acggcgaact tcacttggcg
     7861 ggtcaggctg tcggagaaga cggagacgcg gtcgagggac gacgccaggt cgaccgtctt
     7921 cacgtgcagg tgcttgttgt tgttcttggg cagcacctgt tcgtagttgg ggaacggacc
     7981 ttcgatcagc ttcgtcgtga gcgaggtttt gcccaccgtg aaccgcgcgt ggttcttgga
     8041 cagctcgacg tgcaccggat cggcgctctc cgccaccagc cggtgcagat gggtcaacgc
     8101 cttcagcgga acgatgacgt cgcccttcaa ggcgcctttc accgcgcccg gatgcttcag
     8161 cgtggcgcgc gcgaggcgat gtccgtcggt cgcgacgagc cggatctctc cttccgccac
     8221 ctggagcagg gcgccgttca gcgcgggacg cgtctcgtcg gtggagacgg cgtagatcgt
     8281 gcgcttgatc atccgctcga gctgcttcgc atcgatcgtc agcgccgtgt cgggcgccac
     8341 gttcacgcgc gcgggaaact cctcggggcg gatgcagagg aacttgaacc gtcccgattt
     8401 gctgacgacg ttcagcgtca gatccttcgc ttccagcttc agctcttcct tcggaagctt
     8461 gcggaccagc tcgaggagct tcttggccgg caccgtgagg gccgcctcgg ctttgacgtc
     8521 ggccatcgcg cgggtcgtga ccgaaaggtc caggtcggtg gccgtcatct gcaaaccttc
     8581 gtccgtggct tccacgagaa tattggcgag cacgggaagc gtcgttctgc tgggaacggc
     8641 gctcgagatg aggctcaacg cctgcaacag ctccgtctgc gcgatggagc atttcatggc
     8701 cacgtcctgt gcgattgtca tgtgtctaag tccctttagc tattggtggt tgcggtcgcg
     8761 tcggggtggc tggttcctcg ctctggatta taggttttcg gggggtcttg gaacaacagg
     8821 aaaggggtca ccttttcggg aggttttcca cacggttcag ggggcctaag acaagatagg
     8881 taccatgaat agaagcagta gttgtagggg gtgtggacgg tgggggcggg aacagggctg
     8941 gcatgggtaa tttgttgtcc cgcttgccat tcccaaattc gtttcggtcg cggggaggta
     9001 gggacgggat ggggatggtg gcgaggttgt cccgatccgt ccccaggacc gcggcggagc
     9061 tcggaaaaaa gccctccgtc cacgggccat ccacaggaaa tcgaaacggg cgccgctctc
     9121 gcgacgcccg tcatttcgcg accgcacgaa cgcgcgcggc cgatgttttg gatcggtcga
     9181 attaggcggt gagctgcgcc atcagctctt cgaccacgcc gcgcatctcg ggatcgttcg
     9241 atatcagcgc ctcgatcttc tggcaggcgt gcagcaccgt cgtgtggtcg cgcccgccga
     9301 acttggcgcc gatctcggcc agcgagagat cggtgagcgt gcgcgcgatg tacatcccga
     9361 tctggcgcgg gaacgcgatc gtatcggtgc gccgcttgcc gcgcagcgat tcctcggtca
     9421 cgttgaaatg ccgcgagacg atccgctgga tgtcgtgcac gtcgaagcgg accaggtcgg
     9481 gcttgatctg atcccgcagc acgtcctgcg cgaactccac ggtcagcttg gaattggtga
     9541 gctcggcgaa agccaacagc cgcgccagcg atccctcgag gtcgcggatg ttgttcttga
     9601 tgttgctggc caggagcagc gcgatctcgt tcgggaccat ccgattctgc agctcggcct
     9661 tctttttgac gatggcgacg cgggtttcca ggtcgggagg ctggatgtcg gccaccacgc
     9721 cccacgtgaa gcgggagagc aggcgctctt ccagcgccgt catcgaattg ggcggaccgt
     9781 cgctggtcag cacgatctgc cggtgctggt cgtggagcgc gttgaacgtg tggaagaact
     9841 cttcctgcgt cgactccttg ccttcgagga actggatgtc gtcgatcagg aggagatccg
     9901 cggaccggta gcgcgccttg aactcgagcg tggtggcgtg ctggatggca tagatcatct
     9961 cgttcatgaa cttctcggcc gagacgaaga acactttcgc gtgcggccgt tgcgcgcgca
    10021 cgaaatggcc gatggcgtgc atgaggtgcg tcttgccgag gccgacgccg ccgtacaaga
    10081 agagagggtt gtagcgctcg ccggggctct gcgcgaccgc ctggcagacg gcgtgcgcga
    10141 actggttccc gttcccgacg acgaacgtgt cgaacgtgtt ctgcggcacg agcgcgctct
    10201 cgtcgcgcga ggggaagccg ccgctccatc cggacgtcct ggcgggacgc gactcctcgc
    10261 gtccgccggg gccccccggt cctatgaagt cctctttcgt gaatgcctgg cccgcgcccg
    10321 aggagccgtt cggttccgac ggcaaagcac cccagggagg ggcgtcggag gcgtcctcct
    10381 ggcgcacctg gaacgtcacg gggatcgtcg cgcccgccgc gctcgcgacc gaggtctcga
    10441 tcagcggccg gtagtgctgg tccagccagt ccacgtagaa ctggctgggc acctcgagga
    10501 cgagcgatcc gttcagcgag accgggcgga gagaccggaa ccacgtttca tacgtctgcg
    10561 gcgaggtgct gtcctgaatg cttccgagtg cgcgttccca gagatcgacg gcggccttct
    10621 cgatcatttc actgccccca cattcacaca cccactccac gcacaatcca cacacttatc
    10681 cacaaaataa ctagggcgta acggattgcg tgtgactccg ttacgccccg agaacgcgat
    10741 gtcgggcaac gcgtgttctg acggcatttc gactttcgat gatggcgcgc gaggcgcgcc
    10801 gggcgagaag tgaagcgggc gctggccgcg atccgagggg atcgcgtcgg acgtccgccg
    10861 aggggtcgga atgtacggcc cacgatccgc gacctgcaac gaaattgttt cggacgcccg
    10921 tcacctgggg gcgacggcgc gtgttgaccg aacggaacgc gtcggttaga ctcgccgact
    10981 cgcaacgaac gacactcact cacacgatcg cgactcgagg agctccaagt gaagcgcacg
    11041 ttccaaccgc acaaccgccg ccggaagaag gtccacggct tccgcgagcg catgagcacc
    11101 cgcggcggcc gcgcggttct gaagcgccgc cgggaccggg gccgcaaacg tctcacggtc
    11161 tagcacggcc gcgcccggcg attcggccat ggccggcgga gttccgactc gcgagcgccg
    11221 tctccgttcg ggatggcagt tccgcgaggt ctaccgcgcc ggcagctcct atcacggcac
    11281 cttgatgtct ctggtcagcc tcggcaagcc cgaagaccac ggccgctggg cgttcgtggc
    11341 cagccgcaag gttggccccg ccgtgcgccg gaaccgcgcg aaacggctcc tgcgcgagag
    11401 cttccgccgc ctgcccgagg atcgccggaa cgagccgcgc tggcgcgtct ggatcgcgcg
    11461 ggccgcctgt tcgcggtccg acctccacca tgtcgaggcc gagatggcgc gtctcgtgaa
    11521 gcaggagcgc cggtgacccg cgccgcggcg gcgatccgct ccggcttcgg cctccgcgcg
    11581 ctcgcgatcc tcctcgtgcg cgcctaccgg ttcgcgatcg cccccgttct gcccaagagc
    11641 tgccgcttca ccccttcctg ctcggcctat gccgaggagg cgctccacaa gcacggtctc
    11701 ttccgcgggc tctggctcgc cgcgaagcgc attctccgct gccatccgtt ccaccccgga
    11761 ggctacgacc ccgtgcccta gggcggggga cgacttcatg gatcgaagaa cactgctcgc
    11821 ggtcgtcctg atcatggccg tgctcctggt cgaccaggtg atctggtcgc gttggaccaa
    11881 gtcccgtcag ccggtcgtcc cgcccacggc cggcggctcc gcggttccgg gcgattcggc
    11941 gacggctcgc gccgcgctcg ccgattcggc cctgcacggc gtcccggcgt cgcagcctgc
    12001 cgaaggcgct tcgtcggccg cgcgcgtcgg cgctccggcg gtcacgggcg gcggtccgct
    12061 gatctcggcg cgtgtcgccg gagcgccggt caccgaggac tcgctcgcca ccgggcagtt
    12121 ccgcgcgcgc ttcaccagcg agggcggggc ggtctcctcc tgggtgctgc ccgggtaccg
    12181 ggatccg
//
LOCUS       DASSLZ010000134        14828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1440515, whole genome shotgun sequence.
ACCESSION   DASSLZ010000134 DASSLZ010000000
VERSION     DASSLZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14828
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440515"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VFM00_05075"
     CDS             <1..503
                     /locus_tag="VFM00_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7497653.1"
                     /translation="VPQVDLLIVLMIAILFLLGLRWEAVAAMFAALGPVIGSLIKLIV
                     ARPRPSADVVHVLRRLDTLSFPSGHVLLATAFYGFIAFLIYALLRPGLLRTILLVIFG
                     LFIALMGPSRIYLGQHWFSDVMGAYVLGSLWLALSVRVYRWGKSRFFRSQPVAAEEPV
                     ESAGRR"
     gene            complement(486..2102)
                     /locus_tag="VFM00_05080"
     CDS             complement(486..2102)
                     /locus_tag="VFM00_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497654.1"
                     /translation="MTRAVRIAAIVSAMTAMIPSAVPAQIVNVQAGASSLQDAQGGAL
                     SIRDDGYEGSLGLGSLSPLRVGAFLRTRWRGADLTVGDQTIPFGLPTDVFAAGHSFLG
                     RGMGVRVKDEEVSLLAMGGTTATGFATPYSFGARSEEPVGLLFLEGRLSPTLLVSSRS
                     LYSGRMTSLFGADWTPRADFRAAVTGGAGAEEGYLAASSRYERGPFAIRAAYALAGSG
                     FRRVVTPRPDASELDRENVEIAFRPRSGPAITAARNRYAQPGSITGTGTVNQLSASGV
                     VRGVATNAALLVSRGPGTGGTGLTLSLGRGVGRLLRLQGSALRSDPLVGKSSTMWVGS
                     VAEAVTPRLEILQLATHASGNTTVSFGGNLLLDAVTLGAEYQTIYVPFAPRDPFRQAL
                     MLHARVQAPGGVQANLGSYVGPDGAVRYTVYASQYFYGGSKGQAEPEGRLYSNLIRGR
                     VQDVTGGPIAGAALHIDKETVFTDSKGEFFLRVKKRREYSLAVATDEFLTPGRYDVIS
                     APATVEAQPEEKAGTILVVVDRVAGLPPAR"
     gene            complement(2099..2860)
                     /locus_tag="VFM00_05085"
     CDS             complement(2099..2860)
                     /locus_tag="VFM00_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497655.1"
                     /translation="MRTRARGGIRAALAPLGPAGALLLAVLALAPASPARAQTLRPVV
                     VEYFGTTAKGKFELANDGLVPLNVVLEPKSFDVTETGDAIYRALDPRIHLRLSTMSAR
                     IPARQSRWVFFEAQTDSIPSWFVITATFSGMPKHAGLDVLVELPHTVYFVQKDLLRRE
                     DVRVTTAVYSEPEKWILIELENRGPRLGRATQIEATAKGERRPYSSFPLVPGGRRQIL
                     IPWDAVREPERVTVRFQGFTLDIPLQKIAQVAGPR"
     gene            complement(2910..3476)
                     /locus_tag="VFM00_05090"
     CDS             complement(2910..3476)
                     /locus_tag="VFM00_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497656.1"
                     /translation="MRIQKAWFGGLVALAVSAALSSTASAQAISNIGNVNLNATLPQS
                     VTVSVTSGSSVNFALTENAATNGSVPTVIQTAWNVNPGQVGAVTLYGYFDVPAQALTD
                     GGGNNIPTSWVEGQMTTGTPTTYTTFTQTNPSGPAGGSLSLFSNAITGLNKISSRTDN
                     LDLRINLTGQTLPAGTYTGVLRLQARAL"
     gene            3721..4848
                     /locus_tag="VFM00_05095"
     CDS             3721..4848
                     /locus_tag="VFM00_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7497657.1"
                     /translation="MIHLTAHQLSTYLDRELPESSTEIVRRHLEGCAECALRFNTFRE
                     QEAILDRVLMHDPGEDFFSGFGDRVLGPGRKQPPALERIEAAPPRPKPAAVEPESEPR
                     MVAPSAAKNRPERQLAIPWFAAAILCLIVGAIGYTIPRPSGIRSPVSAVEHPAPRAAL
                     TQHALRPEPLAETMPTPEIPARSAETRAGTEVSPPSPPETASPSVETPARAPESPAHS
                     IRETAIVAPEPSVAPVRRFHTTTSVVPSPAPARPQRRPAIENPAPAAVPDEFAGAPPS
                     AAPLLAGARRASQAAAADSSAANLDAAAEAWDQAVSSLSGAEQTTARRHLAESRYRAW
                     VAAPDAYRAASAIASLRAFLVLTPPSPERDLAKEQLRRLGG"
     gene            4927..5751
                     /locus_tag="VFM00_05100"
     CDS             4927..5751
                     /locus_tag="VFM00_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET7497658.1"
                     /translation="MIIGLASPRAATTFEDGLERIERLTTDAAAQGARVVCFPEAYLP
                     GLRGQDFEVMPWDREREERALRVVGSWAKAHRIAVILGLEHVADAGRQIAAFVVDADG
                     SALGCQIKTQIDPSEDGFYVPGSERRLFEADGMKFGIAICHEGWRYPETVRWAAMRGA
                     KIVFHPQHTGSNTAGAVPTAWGAPEGAYYEKAMMMRSIENTIYFASVNYALRYPEAAT
                     SLIDPSGRCQAYLPYSEEGVLVQELDLKKATGLLASRYAPERYMEAAVGTERETTT"
     gene            5748..6023
                     /locus_tag="VFM00_05105"
     CDS             5748..6023
                     /locus_tag="VFM00_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497659.1"
                     /translation="MNGSTIRTRHPQGKSGKNIAKEKYEAVKTAMLSALRGRELTHPE
                     LMQALESNLKGNFDGNVHWYGETVKLDLESRKIVERTATKPQKYRLR"
     gene            complement(6154..6693)
                     /locus_tag="VFM00_05110"
     CDS             complement(6154..6693)
                     /locus_tag="VFM00_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018908981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7497660.1"
                     /translation="MTFDLQPTLTGDLLELRPLRPEDFGPLHAVAADPLIWEQHPESD
                     RHREERFRVYFDDALKSGGALIARDRADGAIIGASRYHGYNAAASEIEIGWTFLARSR
                     WGGRYNGEMKRLMLDHAFRFVERAVFLIGENNWRSRKAVEKIGGVYAGTRIDGKRQER
                     VVYAITADAWRRRLAEGSP"
     gene            6978..7514
                     /locus_tag="VFM00_05115"
     CDS             6978..7514
                     /locus_tag="VFM00_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497661.1"
                     /translation="MSTGPNRAGAIAALVVIGVFCGACQKDGGMRPATAPLSNGAALL
                     STASRAGAAASGGSTKGAAGEMPAYYDAQLFTINSVELPEDASAATIAKNQSLNEIFA
                     TNDLDDPQDFFPVISAIQGDGFNPLWRQILIVFNPGVTPHQFFSDDEVRAAATGDHPE
                     ITLNVTDEVYRCSVVGSK"
     gene            7632..9173
                     /locus_tag="VFM00_05120"
     CDS             7632..9173
                     /locus_tag="VFM00_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497662.1"
                     /translation="MKTLGFVLAALCALSIPMAARAHEEHVHEAPNPETLGTVHFPVS
                     VDDAAQREFDRAVALLHSFEYEQAERGFRSLTERDPGCAMAYWGIAMCNYHPIWGPTT
                     QADFERGRAAADTAASLGGKTDRERSYIAAIGTYYKDADRLDAPTRRIAYEKAMEQLH
                     ARFPDDVEGSIFYALSILGNASTTDKTYAVQRRAADLLNAVLPKAPNHPGVAHYLIHS
                     LDYPGLASLALPAARAYAKIAPSAPHAQHMPSHIFTRLGLWDESIQSNLAAQKASQDL
                     MARTHPGTTYFEELHAMDYLAYAYLQLGRDAEAKGILDAMRAISHLNQQTPQAAYAFA
                     AVPARYALERHRWKEAARLTLQPTWFTWDKYPWAEALTQTARGIGAARSGNLRVARQA
                     LARLDSLHAALAGVKDGYNWADQVEVERREVAGWMARAEKKDDEALKQMRSAADLEDS
                     IDKNPVTPGALLPAREQLGDLLMELGTPGDAIAAYEEVLKSSPGRRNSLRGLEQAKRS
                     VVSSP"
     gene            9185..9691
                     /locus_tag="VFM00_05125"
     CDS             9185..9691
                     /locus_tag="VFM00_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497663.1"
                     /translation="MNTVAQALTEIFREIVQGSPDTSARTIVLNQGDPGLLASLERLS
                     AAAASATHDGGASIASHVDHLRYGLSLLNRWADGLSPPWPEMDWTASWRQTAVSDAAW
                     RTLRDDLRREANRWGQALSTPRDLNDVEAGWMAGSVVHLAYHMGAIRQIDRATRGPTA
                     EDEATHRA"
     gene            complement(9688..11193)
                     /locus_tag="VFM00_05130"
     CDS             complement(9688..11193)
                     /locus_tag="VFM00_05130"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HET7497664.1"
                     /translation="MSVATAKHRPSVRLFAPDEPERVVLEACRVLERVGVLVENEQGR
                     ALLSGAGAQERGGRWRIPEALVRAALASAPRQIVLYDVTGQPVCDLGEGRVHFDPGSA
                     AIHVLDPAQGRRSATVEDVTRLVRLVDRLPHYAAQSTALVPSDVPVPMGDRVRLYLAL
                     RHGRKPVITGTFRKDGFAPMRAMLECVRGSAEALAEKPLAVFDCCSTPPLKWSDLTCQ
                     ALLDCARARIPAELVAMPLTGATAPVTLREAVIQHGAESLSGIVLHQLARPGAPIVYG
                     GAPSAFDMRHGTTTMGAIETMMIVIGYAQVGKHLGLPTHGYLALSDAKRPDYQAGMET
                     GIGAVLAALAGINMISGAGALDFILTQSLEKLVLDHDACGMALRLARGIERRDGDITG
                     LFAELVQRGEFLSHPHTRKHWREELSMSSGCVDRGSYGDWESDGARTAEQRAADEVAR
                     LLAKEAQAALPEATLEALREILEAEGQRFGWEMPHPTRLAAGPTFNSQPTP"
     gene            complement(11190..11858)
                     /locus_tag="VFM00_05135"
     CDS             complement(11190..11858)
                     /locus_tag="VFM00_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497665.1"
                     /translation="MTHAGLAPLPVGIQRLDVLHFLGYRGQGLPSARVAQALGEVMEQ
                     GRSLVTACGVHRTVRADEVASLGLVPIPRAALALGLVTIGPALEREVSWLLARGETTR
                     ALLLDAVGSAAVEEAADLLEGRMRERVIGGSSRAGTASRRFSPGYGRWPLTAQPALFA
                     LVPHAEIHVDLLPSCIMSPRKSVSFAVWLGPGDDPHAPEGCRARRCAECDLTNCLYRE
                     PDPS"
     gene            complement(11855..12751)
                     /locus_tag="VFM00_05140"
     CDS             complement(11855..12751)
                     /locus_tag="VFM00_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET7497666.1"
                     /translation="MTDLLERLRRGPCLLLDGGFGSELIARGLEAGASPDRWTLERPA
                     DVLGVHRAYVEAGSEAIHANTFGANRARLERHGLAGRIEEINHAAVRLAREAGAPYVF
                     ADIGPSGEHLPPVGRANLDEWRGAFEEQGRILADTGVDAFHVETMTDLREGLTALESL
                     RRAAPKIPVLVSLTFERKKRGFFTLMGDPLLGSLTRLRDAGAAAVGANCTLASGDMRD
                     LAREALASTDALLAFQPNAGTPVLAGGRTTYAQSPGEFAEDLAPLAITPRVAALGGCC
                     GTDPRFIRALRARMSRPAAVPA"
     gene            complement(12748..13389)
                     /locus_tag="VFM00_05145"
     CDS             complement(12748..13389)
                     /locus_tag="VFM00_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HET7497667.1"
                     /translation="MPPLDYAPLSKAVIDGDVEESARLARAWVAAGGDPLQAVEQGFS
                     EGIRRVGELWEEGEYFLPELVQGADAMKAALEALRPWLGARSNAGHGRGCVVIGTVEG
                     DLHDIGKSLVATMLSAYGFEVHDLGSDVSVARFVEEARRHAADIVAASALLTTTMVIQ
                     RELAAAIQAAGLPRSPRILIGGAPTTVAWAEEIGALHAENAMRAVAVAEGALR"
     gene            13596..13925
                     /gene="merB"
                     /locus_tag="VFM00_05150"
     CDS             13596..13925
                     /gene="merB"
                     /locus_tag="VFM00_05150"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HET7497668.1"
                     /translation="MLAAYRVLRAQRLLLLEPDGTTIRMAPPFSGVPTQHRVESGGTS
                     YFANCAWDALGIPAALRRPGRVHSLCEQSGEPLDLSVERNGPEPSPWLFHCSVPAARW
                     WDDLVFT"
     gene            14010..14162
                     /locus_tag="VFM00_05155"
     CDS             14010..14162
                     /locus_tag="VFM00_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497669.1"
                     /translation="MDQLWSLATTWYATRLDEDSRRPKAEEMRGIFASIGLDDPFWDP
                     SADLFA"
     gene            complement(14246..>14828)
                     /locus_tag="VFM00_05160"
     CDS             complement(14246..>14828)
                     /locus_tag="VFM00_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497670.1"
                     /translation="LLTPLLVAGVPHGARAADVGISIRIGDRYQGSELRFTNRPRLVV
                     IPRTNVYYVQNYDVDVYRYGRYFYAYDRGRWWRATSYRGPWYYIRGRAVPRQIFIIPA
                     DYRRNWQGDYGEWRDRDYDQNWEQYWGHGGRGREVNRAQEVKRGPEMNRGQDMRGHDM
                     NRGANPSDQGNQKDKGKRGNDKGGNKGHGNSGH"
BASE COUNT         2334 a         5169 c         5063 g         2262 t
ORIGIN      
        1 tcgtaccgca ggtggacctc ctgatcgtgc tcatgatcgc catcctgttc ctgctgggcc
       61 ttcgatggga ggcggtcgcg gcgatgttcg ccgcgctggg cccggtcatc ggatcactga
      121 tcaagctgat cgtcgcgcgc ccccgtccca gcgccgacgt cgtccacgtg ttgcggcggc
      181 tggatacgtt gagcttcccc agcggacatg tgctcctggc gaccgcgttc tacgggttca
      241 tcgcgtttct catctacgcg ctcctcaggc cgggcttgtt gcggacgatc cttctcgtga
      301 tcttcgggct cttcatcgcc ctgatgggac cctcgcgcat ctaccttggc cagcattggt
      361 tcagcgacgt gatgggagcg tacgtgctgg ggagcctgtg gctcgcgctc tcggtgcggg
      421 tctatcggtg gggaaaatcg cggttcttca ggagtcagcc ggtcgcggcg gaagagccgg
      481 tcgaatcagc gggccggcgg tagcccggcc acgcgatcca cgacgaccag gatcgttccc
      541 gccttctctt cgggctgagc ctccacggtg gccggcgcgg agatcacatc gtagcgtccc
      601 ggcgtcagga actcgtccgt cgcgaccgcc agcgagtact cgcggcgttt cttcacgcgc
      661 aggaagaact cacccttcga gtcggtgaac accgtctcct tgtcgatgtg gagcgcggcg
      721 cccgcgatgg gcccgcccgt cacgtcctgg acgcggcccc ggatcaggtt ggagtagaga
      781 cgtccctccg gctccgcctg tcccttcgat ccgccgtaga agtactggct ggcgtagacc
      841 gtgtagcgca cggcgccgtc cgggcccacg tagctgccga ggttcgcctg gaccccgccc
      901 ggcgcctgga cgcgcgcgtg cagcatcagg gcctggcgga acgggtcgcg cggcgcgaac
      961 ggaacgtaga tcgtctggta ttcggcgccg agggtcaccg cgtcgagcag gaggtttccg
     1021 ccgaagctca cggtcgtgtt gccggaggcg tgagtggcga gttgaagaat ctcgaggcgc
     1081 ggcgtgaccg cctcggcgac cgagccgacc cacatcgtcg acgacttccc cacgagcggg
     1141 tcggagcgca gcgcgctccc ctggagccgg aggaggcggc cgacgccgcg gcccagggag
     1201 agggtgaggc ccgtgccgcc cgtgcccggc ccccgggata cgaggagtgc ggcgttcgtc
     1261 gcgacgccac gcaccacgcc cgacgcggaa agctgattca ccgttcccgt gcccgtgatg
     1321 gagcccggct gcgcgtaccg gttcctcgcg gccgtgatcg cgggcccgga ccggggccgg
     1381 aacgcgatct cgacgttctc ccgatccagc tcggaggcat cggggcgcgg cgtcaccacg
     1441 cggcggaagc cggagccggc cagggcgtag gccgcgcgga tcgcgaacgg tccgcgctcg
     1501 tatcgggaac tcgcggccag atatccctcc tcggcgccgg cccctccggt cacggccgcg
     1561 cggaagtccg cgcgcggcgt ccagtccgcg ccgaagaggc tggtcatgcg cccggagtac
     1621 aggctgcgcg aggagaccag gagcgtgggc gaaaggcgcc cctccagaaa gagcaggccg
     1681 accggttctt ccgagcgcgc cccgaacgag tagggcgtcg cgaagccggt cgccgtcgtc
     1741 ccccccatgg ccaggagcga gacctcctcg tccttcacac gcacgcccat cccgcggccc
     1801 aggaatgaat gacccgccgc aaagacgtcg gtgggaaggc cgaaggggat ggtctgatcg
     1861 ccgacggtaa ggtccgcgcc gcgccagcgc gtgcgcagga aggctccgac gcggagcgga
     1921 ctcaggcttc cgagaccgag cgagccctcg tatccgtcgt cgcgaatgga gagcgccccg
     1981 ccctgggcgt cctgaagcga cgaggcgccg gcctggacgt tgacgatctg cgccgggacg
     2041 gcggagggga tcatcgccgt catcgcgctg acgatcgccg cgatgcggac ggcccgggtc
     2101 accgcggtcc cgcgacctgg gcgatcttct ggagggggat gtcgagggtg aagccctgga
     2161 agcggaccgt gacgcgctca ggttcgcgca cggcatccca cggaatgagg atctggcgcc
     2221 ggccgccggg cacgaggggg aagctggaat acgggcggcg ctcccccttg gcggtcgctt
     2281 cgatctgcgt cgcgcgcccc agccgcggcc cacggttctc cagctcgatc aggatccact
     2341 tctccggctc gctgtagacc gccgtcgtca cgcgaacgtc ctcgcggcgg aggaggtcct
     2401 tctgcacgaa gtagacggtg tggggcagct cgacgagaac gtcgagcccc gcgtgcttcg
     2461 gcatgccgga gaaggtggcc gtgatgacga accaggaggg gatggaatcg gtctgcgcct
     2521 cgaagaagac ccaccgggac tggcgggcgg gaatccgggc gctcatggtg gagaggcgca
     2581 ggtggatgcg cggatcgagc gcgcgataga tcgcgtcgcc ggtctcggtc acgtcgaagc
     2641 tcttcggctc gagcacgacg ttcagtggca ccaggccgtc gttggccagc tcgaacttcc
     2701 ccttggccgt cgtgccgaag tactccacca cgaccgggcg aagcgtttgc gcccgggcag
     2761 gactcgcggg cgcgagcgcc aggacggcca ggagaagagc gcccgcgggg ccaagcggcg
     2821 cgagggcggc gcggatccct ccgcgcgccc tcgtccgcaa agagttcctc cctggaaccc
     2881 ggtccaacct cgttccttcc cggcgcgccc tagagggccc gcgcctggag tcggagcacg
     2941 cccgtgtagg tgccggcggg gagcgtctgg ccggtcaggt tgatgcgcag gtccaagttg
     3001 tcggtccggc tgctgatctt gttcagaccc gtgatcgcgt tcgagaagag gctcaggctc
     3061 ccgccggcgg gccctgacgg gttggtctgc gtgaaggtgg tgtaggtggt gggggtgccc
     3121 gtcgtcatct gcccttccac ccaggaggtc ggaatgttgt tgccgccgcc gtcggtcagc
     3181 gcctgggcgg gcacgtcgaa gtagccgtac agcgtcaccg cgccgacctg ccccggattc
     3241 acgttccagg cggtctggat gaccgtcggc acgctcccgt tcgtggcggc attctcggtg
     3301 agcgcgaagt tcacggacga gccgctcgtc accgacacgg tgaccgactg cggcagcgtc
     3361 gcgttcaggt tcacgttccc gatgttggaa atcgcctggg ccgatgccgt agacgagagc
     3421 gccgccgaca ccgccagcgc gacgagaccg ccaaaccatg ccttctggat ccgcatacct
     3481 gctcctccgt tgaggtggcg ggggcccccc actggattgc cccccgacga ccgcggctgc
     3541 tatagccgtt tttcggcgtc accgccgcca tcttgagcgg gtatccagga acgtctccga
     3601 agacgtccac gtggcgtctt gataacgtct ccaggacgca agatcgggcc gcctcctgcc
     3661 gatcgacgac gacagggatc ttcccgaagt gtcgtcgcgc gaccacggag cacccaagac
     3721 gtgatccacc tcacggccca ccagctttcc acctatctcg accgcgagct gcctgaatcg
     3781 tcgacggaaa tcgtccggag gcatctcgag ggctgcgcgg aatgcgccct gcggttcaac
     3841 acgttccggg agcaggaggc gatcctcgac cgggttctga tgcacgatcc cggggaggat
     3901 ttcttcagcg gatttgggga ccgggtgctc ggccctggcc ggaagcagcc gccggcgctg
     3961 gaacggatcg aggcggcgcc gcccagaccc aagcccgccg ccgtagagcc agagtccgag
     4021 cctcggatgg tcgcgccctc cgcggccaag aaccgtccgg agcgacaact tgccattccc
     4081 tggttcgccg cggcgatcct ctgcctgatc gtgggcgcga tcggctacac gatcccgagg
     4141 ccgtccggaa ttcggagccc ggtttccgcc gtggaacacc ccgcgccgcg cgccgcgctg
     4201 acgcagcatg ctctaagacc ggagccgctg gccgagacca tgccaacgcc cgagattccg
     4261 gcgcgctctg cagagacgcg ggcaggcacc gaggtgtcgc cgccaagccc tccggagact
     4321 gcgtcacctt cggtagagac tcccgcgcgc gctccggagt cgccggcgca ctccatccgc
     4381 gagaccgcga tcgtcgcgcc ggaaccgagc gtggccccgg tacgtcgatt ccacacgacg
     4441 acgtcggttg tcccttcgcc ggcgccagcg aggccgcagc gtcggccagc gatcgagaac
     4501 ccggcgccgg ccgcggtccc cgacgagttc gccggcgctc cgccttcggc cgcccccttg
     4561 ctggccgggg cccggcgcgc ttcccaggcc gcggccgccg actcctcggc cgcgaatctc
     4621 gacgccgccg ccgaggcctg ggaccaggcg gtttcgtcgc tgagcggcgc cgagcagaca
     4681 accgctcggc ggcacctcgc cgagtcgcgg taccgggcgt gggtggccgc tccggacgcc
     4741 tatcgggcgg cgtcggccat cgcttcccta cgcgcctttc tcgtcctcac tcccccctct
     4801 ccggaacgcg acctcgccaa ggagcagctc aggcgtctcg gcggctgaag aaggacgtgc
     4861 ctccctgcgg agacctcggg taaccttccc gcttcacggc gtcatcccga cccacgaggt
     4921 tcccccatga tcatcggatt ggcctcgccc cgcgcggcca ccacgttcga ggacggcttg
     4981 gagcggatcg agcgcctgac cacggacgcc gccgctcagg gcgcgcgcgt cgtctgcttc
     5041 cctgaagcgt atctgccggg actgcgcggg caggacttcg aggtcatgcc ctgggatcgc
     5101 gagcgcgagg aacgcgcgct tcgggtcgtg ggttcctggg ccaaggcgca tcgcatcgcc
     5161 gtgattctcg gcctggagca cgtcgcggac gccggccggc agatcgccgc gttcgtggtc
     5221 gacgccgacg gcagcgccct gggctgccag atcaagacgc agatcgatcc gagcgaagac
     5281 ggcttctacg tgccgggatc ggagcgacgc ctgttcgagg cggatgggat gaagttcggc
     5341 atcgcgatct gccacgaagg ctggcggtat ccggagacgg tgcgctgggc ggccatgcgc
     5401 ggcgcgaaga tcgtgttcca tccccagcac accgggagca ataccgcggg cgccgttccc
     5461 acggcgtggg gggcgccgga aggcgcgtat tacgagaagg cgatgatgat gcgcagcatc
     5521 gagaacacga tctacttcgc gagcgtgaac tacgccctgc gctatcccga ggcggcgacc
     5581 agcctcatcg acccttcggg gcggtgccag gcgtacctgc cctacagtga agaaggcgtt
     5641 ctggtccagg agctcgatct gaagaaggcg acgggactct tggcctcgcg ctacgcgccc
     5701 gagcgctaca tggaggccgc cgtcggcacg gaaagggaga cgacgacatg aacggttcca
     5761 cgattcggac ccgccatccc caggggaaga gcggcaagaa catcgcgaag gagaagtacg
     5821 aagcggtcaa gacggcgatg ctgtccgccc tgcggggccg cgagctcacg catcccgagc
     5881 tgatgcaggc gctcgagagc aacctgaagg gcaacttcga cggaaacgtc cattggtacg
     5941 gcgagaccgt gaagctggat ctcgagtcgc ggaagatcgt ggagcgcacc gcgacgaagc
     6001 cgcagaagta ccgcctgcgc tgagacggga gctcagccct gcagaccagc tccggtactg
     6061 ccgccggtgt actcgatgac cgtgttcaaa cggacgatcg aggtgttgcc gaccgcgtcg
     6121 aggatcttca tatcagcggg cgccgccgga gcctcacggc gacccttcgg ccaggcgccg
     6181 gcgccatgcg tccgcggtga tcgcgtagac cacccgctcc tgccgcttcc cgtcgatccg
     6241 ggtgcccgcg tagacgccgc cgatcttctc gaccgccttc cgcgagcgcc agttgttctc
     6301 gccgatgagg aagacggcgc gctcgacgaa gcggaaggcg tgatccagca tcaaccgctt
     6361 catctcgccg ttgtaccggc caccccaccg cgatcgcgcg aggaacgtcc agccgatctc
     6421 gatctcgctc gcggcggcgt tgtagccgtg atagcgggag gctccgatga tcgcgccgtc
     6481 cgcgcgatcg cgcgcgatca gggcgccccc cgacttcaag gcgtcgtcga aatacacccg
     6541 gaatcgctcc tcgcgatggc ggtccgactc cgggtgctgc tcccagatga gcggatcggc
     6601 ggcgaccgcg tggagggggc cgaagtcttc cgggcgaagc gggcgaagct ccagcagatc
     6661 tccggtcaac gtgggctgca ggtcgaaggt caatcgttcc tctcgggtgg cggtccgggc
     6721 gaaagacgaa agcgaaaccc tatcagattc ggagcgccgg ccggatcgaa tgtgccgagc
     6781 cgcaccttcg ggcgcgtgac ccaggtcaca ttcgctccgc agcgtatcga aattcgcgct
     6841 aaacgtgaat cggcgagcat cgcgcgcggt gtccgcgcat ctcacgaata tcgtcgcgac
     6901 gccgtcaact ccggagcggg acttggtttg tgtgaccgcg atcacgttgt tccaccgcca
     6961 tcatgaagga gaccaccatg agtacgggac ccaatcgagc gggcgccatc gcggcgctcg
     7021 tggtcatcgg cgtcttctgc ggcgcctgcc agaaggacgg cggcatgcgg cccgccaccg
     7081 cgccgctgtc gaacggagcg gcattgctgt cgaccgcatc ccgcgcgggc gcggcggcct
     7141 ccggagggag caccaaggga gccgccggtg agatgcccgc ctactacgac gcgcagctct
     7201 tcacgatcaa ttcggtcgag ctgccggagg acgcttcggc ggcgaccatc gccaagaacc
     7261 agagcctgaa cgagatcttc gcgacgaacg acctggacga cccccaggac ttcttccccg
     7321 tcatcagcgc catccagggg gacgggttca atcccctctg gcggcagatc ctgatcgtct
     7381 tcaacccggg cgtgacgccg catcagttct tctccgacga tgaagtccgg gcagcggcca
     7441 cgggcgacca tccggagatc acgctgaacg tgaccgacga ggtctatcgt tgctcggtcg
     7501 tgggatcgaa gtagagcggg tgagcgggcc gggtgcgcgc gtcgcgtcca ctcggcccgc
     7561 accgccggca cccctgaaga acgatcccga agggtagccg aggccccatc ccgcggctat
     7621 actcgccgcc gatgaagacc ctggggttcg tactcgccgc gctctgcgcc ctctcgatcc
     7681 cgatggccgc gcgcgcccac gaggaacacg tccacgaggc gccgaatcct gagacgctcg
     7741 gcaccgtgca cttcccggta tcggtggacg acgccgcgca gcgggagttc gaccgcgcgg
     7801 tcgccctgct ccactcgttc gagtacgaac aggcggagcg cgggtttcga tccctgaccg
     7861 agcgggatcc gggctgcgcc atggcgtact ggggaatcgc catgtgcaat taccacccga
     7921 tctggggacc cacgacgcag gccgacttcg agcggggccg ggcggctgcc gacaccgccg
     7981 ccagtcttgg gggcaagacc gaccgggagc ggagctacat cgcggccatc gggacctact
     8041 acaaggacgc cgatcggctc gatgctccca cccggcgtat cgcgtacgag aaggcgatgg
     8101 agcagcttca cgcgcgcttt cccgacgatg tcgagggatc catcttctac gccctttcca
     8161 tcctgggcaa cgcatcgacg accgataaga cgtacgccgt gcagcggcga gccgccgatc
     8221 tcctgaacgc cgtccttccc aaagccccca accaccccgg cgtcgcccac tacctcatcc
     8281 acagcctgga ctacccgggc ctcgcgagcc tcgcccttcc cgcggcgcgc gcctacgcga
     8341 agatcgcgcc ttccgcgcct cacgcgcagc acatgccctc gcacatcttc actcggctcg
     8401 ggctgtggga cgaatcgatc cagtccaacc tcgccgcgca gaaggcgtcg caggatctga
     8461 tggccaggac ccatcccggc accacgtatt tcgaagagct tcatgccatg gactacctcg
     8521 cctacgccta tctccaactc ggccgggacg ccgaggccaa gggcatcctc gacgcgatgc
     8581 gcgcgatctc ccacctcaat cagcagacgc cgcaggcggc gtacgcgttc gccgcggttc
     8641 ccgcgcgcta tgcgctcgag cggcatcgct ggaaggaggc ggcgcggctc acgctccagc
     8701 cgacatggtt cacgtgggac aagtatccct gggccgaggc gctgacccag accgcgcggg
     8761 gcatcggggc ggcccgctcc gggaacctgc gcgtggcccg ccaggcgctc gcccgtctcg
     8821 attcgctcca cgcggcgctc gccggcgtca aggacgggta caactgggcg gaccaggtgg
     8881 aggtcgagcg gcgcgaggtg gcgggatgga tggcgcgcgc ggagaagaag gacgacgaag
     8941 ccctgaaaca gatgcgctcg gccgcggacc tcgaggactc catcgacaag aacccggtca
     9001 cgcccggcgc gctgttgccc gctcgcgagc agctgggcga tctcctgatg gagctgggca
     9061 cgccggggga tgcgatcgcg gcgtacgaag aagtgttgaa gtcgtcgccc ggcaggcgga
     9121 acagcctgcg cggattggaa caggcgaagc ggagcgtcgt ttcgtcgccg taaaggaagg
     9181 gaccatgaac accgtcgccc aggcattgac cgagatcttt cgtgagatcg tccagggctc
     9241 tccggatacg agcgcgcgca cgatcgtgct caaccaggga gaccccggcc tcctcgcgtc
     9301 gctggaacgg ctgtcggcgg ccgccgcttc agcgacgcac gacggcggcg cgtcgatcgc
     9361 ttcccatgtg gatcatcttc gctacggcct gtcgctgctc aaccgatggg cggacggctt
     9421 gtctccgccg tggcctgaga tggactggac cgcgagctgg cggcagaccg ccgtgtcgga
     9481 cgccgcttgg aggacgctgc gggacgacct tcgacgcgag gcgaaccgct ggggccaggc
     9541 gctcagcacg ccccgcgacc tgaacgatgt cgaggccggc tggatggccg gcagcgtcgt
     9601 gcacctcgcc taccacatgg gcgcgatccg acagatcgat cgcgcgaccc gcggcccgac
     9661 cgccgaggac gaagcgaccc accgggccta gggcgtcggc tgggaattga aggtaggacc
     9721 cgccgccagc cgcgtcgggt gtggcatctc ccaaccgaac cgctggcctt cggcttcgag
     9781 gatctcgcgc agggcttcga gcgtggcttc gggcagggcg gcctgggctt ccttggcgag
     9841 aaggcgcgcc acctcgtcgg cggcgcgctg ctcggccgtc cgggcgccgt cggattccca
     9901 gtcgccgtag gatccgcgat cgacgcatcc cgaggacatg gagagctcct cgcgccagtg
     9961 cttccgcgtg tgcggatgcg agaggaactc cccgcgctgg accagctcgg cgaacagtcc
    10021 cgtgatgtcg ccgtcgcgcc gctcgatccc gcgcgccagg cgcagcgcca tgccacaggc
    10081 gtcgtggtcc aggaccagct tctccaaaga ctgcgtgagg atgaaatcca gcgcgcccgc
    10141 gcccgagatc atgttgattc cggcgagggc tgcgagcacg gcgccgatcc cggtctccat
    10201 gcccgcctgg tagtccggtc gcttcgcgtc cgacaaggcg agatacccgt gcgtcggcag
    10261 gcccaggtgc ttccccacct gcgcatagcc gatcacgatc atcatcgtct cgatcgctcc
    10321 catcgtcgtc gtcccgtgac gcatatcgaa cgcggagggc gctcccccgt agacgatcgg
    10381 agcgccgggg cgggcgagct gatggagcac gatgcccgag aggctctcgg cgccatgttg
    10441 gatcacggcc tccctcagcg tgacgggagc ggtcgcgccg gtgaggggca tggccaccag
    10501 ctcggcgggg atcctggctc gcgcgcagtc gagcagcgcc tgacaggtga gatcgctcca
    10561 tttgagcggg ggcgtggagc agcagtcgaa gacggcgagc ggcttctcgg ccagggcctc
    10621 ggcgctcccg cgcacgcatt ccagcatggc gcgcatcggg gcgaagccgt ccttccggaa
    10681 ggttccggtg atcacgggct tccgcccatg ccgcagggcg agatagagcc ggacgcgatc
    10741 ccccatcggg accggcacgt ccgaaggaac gagcgcggtg gattgcgcgg cgtaatgcgg
    10801 gagtcgatcc accagacgca ccagtcgcgt cacgtcttcg acggtggcgg accggcgccc
    10861 ctgtgccgga tcgagaacgt ggatggccgc ggagcccggg tcgaagtgga cgcggccctc
    10921 ccccaggtcg cataccggct gccccgtgac atcgtagagg acgatctggc gcggcgccga
    10981 agccagtgcc gcgcgcacga gcgcctcggg aatcctccag cgcccgccgc gttcctgcgc
    11041 acccgcgccg gagagaagcg ctcgcccttg ctcgttctcg accagcaccc cgacgcgctc
    11101 gagcacgcgg cacgcctcga ggacgactcg ctccggctcg tcaggcgcga acagacggac
    11161 cgatgggcgg tgcttcgcgg tcgcgacgct cacgacgggt cgggctcgcg gtagaggcag
    11221 ttcgtcagat cgcactcggc gcaccgccgt gcgcggcacc cttccggagc gtgcgggtcg
    11281 tctccgggcc ccagccagac ggcgaacgaa accgacttgc gcggcgacat gatgcaggat
    11341 ggaagcaggt ccacgtggat ctccgcgtgc ggcacgagcg caaagagagc cggctgcgcc
    11401 gtgagcggcc agcggccgta gcccgggctg aagcgccgcg atgcggtccc ggcccggctc
    11461 gatcccccga tgaccctctc gcgcatccgt ccctcgagaa gatcggccgc ttcctccacc
    11521 gcggcgctac ccaccgcgtc gaggagcagt gcgcgcgtgg tctcgccgcg cgcgagcagc
    11581 cacgagacct cgcgctccag ggccggtccg atggtgacga ggccgagcgc cagggcggcg
    11641 cgcggaatcg gcaccagtcc caggctcgcg acctcgtccg cgcgcaccgt gcggtggacg
    11701 ccgcaagccg tcaccagcga gcgcccctgc tccatgactt cgcccagcgc ctgcgccacg
    11761 cgagcgctcg gaagtccttg cccccggtag ccgagaaaat ggagcacgtc gagccgctgg
    11821 attccgaccg gcagcggtgc gagtccggcg tgggtcatgc cggcaccgcc gccggccggc
    11881 tcatgcgcgc gcgcagggcg cggatgaacc gcggatcggt tccgcagcag ccgcccagcg
    11941 ccgcgacgcg aggcgtgatg gccaggggtg ccaggtcctc ggcgaactcg cccggggact
    12001 gggcgtaggt cgttcttccg ccggccagga ccggcgtccc cgcgttcggc tggaacgcga
    12061 ggagcgcgtc ggtggaggcg agggcctcgc gcgccagatc gcgcatgtcc ccgctcgcga
    12121 gggtgcagtt ggcgccgacg gccgcggcgc cggcgtcgcg caaccgggtg agcgacccca
    12181 gaagcggatc ccccatcagc gtgaagaagc cgcgcttctt ccgctcgaag gtcagcgaga
    12241 cgaggacagg gatcttgggc gccgcgcggc gaagcgactc cagcgcggtc agcccttcgc
    12301 ggaggtccgt catcgtctcc acgtggaagg catccacgcc cgtgtcggca aggatccggc
    12361 cctgctcctc gaaggcgccc cgccattcgt ccaggttggc ccggcccacc ggcggaagat
    12421 gttcgccgga ggggccgatg tccgcgaaga catacggtgc gcccgcctcg cgcgccagac
    12481 gcaccgcggc gtgattgatc tcttcgatgc gaccggccag tccgtgccgc tcgagccggg
    12541 cgcggttggc tccgaacgtg ttcgcgtgga tcgcctcgct tcccgcctcg acgtacgcac
    12601 ggtgcacgcc gagcacgtcg gcggggcgct ccagcgtcca tcggtccggc gaggcgccgg
    12661 cctccagccc acgggcgatc agctcgcttc cgaaaccgcc gtcgaggagg agacagggac
    12721 cgcggcgcag ccgctcgagc aggtcggtca tcggagggct ccttccgcca cggccaccgc
    12781 gcgcatcgcg ttctccgcgt gcagcgctcc gatctcctcg gcccacgcga cggtggtcgg
    12841 cgcgccgccg atcaggatgc gaggggagcg cggcaggcct gccgcctgga tggcggccgc
    12901 cagctcgcgc tggatgacca tcgtggtggt gaggagcgcc gaggcggcga cgatgtcggc
    12961 cgcgtgccgg cgcgcttctt ccacgaagcg ggcgaccgac acgtcgcttc cgagatcgtg
    13021 cacctcgaag ccgtaggcgg agagcatcgt cgcgacgagg ctctttccga tgtcgtggag
    13081 atcaccctcg accgttccga tcacgacgca gccgcgaccg tgcccggcgt tcgatctcgc
    13141 gccgagccag gggcgcagcg cctccagcgc cgccttcatc gcatcggcgc cctggaccag
    13201 ctcgggaagg aagtactccc cctcttccca tagctcgccg acgcgtcgga ttccctcgga
    13261 aaatccctgc tcgaccgcct ggagcggatc gccgcccgcc gcgacccagg cacgggcgag
    13321 ccgggccgat tcctcgacgt caccgtcgat cacggctttc gaaagcgggg catagtcgag
    13381 gggcggcatg atgtgaggat cggggcagcg ggaccggccc ggatatggtc cggtgggata
    13441 ctcggcgcct cgcgatttgg ccccacccgc tgtagattcg ccggatggac gaagccgcac
    13501 gggtcaagat cgctgtctac gggcatttcg ccgaaatcgg aacccgtccg agcccgagcg
    13561 aggtcgcaca acgcgccggt atcggtgagc cgggtgtgct ggcagcgtat cgggtgctgc
    13621 gcgctcagcg cctgctgtta ctggaacccg acgggaccac gatccggatg gcgcctccat
    13681 tctcgggagt tcccacccag caccgggtcg aatccggcgg aacctcctac ttcgccaact
    13741 gcgcctggga tgcgctcggg attccggcgg cgctccgacg cccggggcgc gttcattcgc
    13801 tctgcgagca gtcgggcgag ccgctcgatc tgtcggtgga gcggaatgga cccgagccct
    13861 ctccctggct ctttcactgc tcggtacctg cggcccgctg gtgggacgat ctcgtcttca
    13921 cctgagcgaa catgctcttc ttccggtcgg aagaacgagt gcgagcctgg tgcgcggacc
    13981 gccgcgaacc gctgcggccg cgggtcacca tggatcagct ctggtcgctc gcgaccacat
    14041 ggtacgccac gcgcctggac gaggattcgc ggcgacccaa ggcggaggag atgcgcggaa
    14101 tcttcgcgtc gatcggcctc gacgatccct tctgggatcc gagcgcggat ctgttcgcgt
    14161 gaggtgactc aaaatagcgc cccaagggac gccatgttcc cttgggcacc cgttcagcgt
    14221 gtgtccctcg ccatatgaag gggcctcagt gtccgctgtt gccgtggccc ttgttgcccc
    14281 ccttgtcgtt gcccctcttg cccttgtcct tctggttacc ctgatcggac ggattcgcgc
    14341 cccgattcat gtcatggcca cgcatgtcct ggccacgatt catctcagga ccacgtttta
    14401 cctcctgcgc gcggttcacc tcgcggccgc ggcccccatg accccagtac tgctcccagt
    14461 tctggtcgta atcccggtcc cgccactcgc cgtaatcgcc ctgccagttc ctccggtagt
    14521 cagctggaat gatgaagatc tgcctcggaa cggcccgccc gcggatgtag taccaaggcc
    14581 cgcggtagct ggtcgcgcgc caccagcggc ctcgatcgta ggcgtagaag tagcgcccgt
    14641 agcgatagac atccacgtcg taattctgga cgtagtagac gttcgtccgg gggatgacca
    14701 ccaggcgagg ccgattcgtg aagcggagct ccgagccctg ataccggtcc ccgatgcgta
    14761 tcgagatgcc aacgtccgcc gccctggccc cgtgcggaac accagccacg aggagaggcg
    14821 ttaacagc
//
LOCUS       DASSLZ010000135         7428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_47418, whole genome shotgun sequence.
ACCESSION   DASSLZ010000135 DASSLZ010000000
VERSION     DASSLZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47418"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..3216)
                     /locus_tag="VFM00_05165"
     CDS             complement(52..3216)
                     /locus_tag="VFM00_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HET7497671.1"
                     /translation="MDKDGRLDVVVHTRNVMTHIDDVSILRNTGGGTLDPRPLLSVGT
                     DAVMGLGDLNVDGRPDVVGAGSGQCTVYLSSGATGFAAPYSVPAAATPLAACVANISD
                     DGNPDLVLLYDNTLDIHQGTGGGLISSSGTSRNLEKAQACGMTPANLDADAQTELVIT
                     HQSLSPGQAEPHGTVELLDFAAPTRWLSTGPWPGGDPVIANLDRDGQLDLVVPSSGHQ
                     VAVVFGPFGTGTGIDQVISGPAPTMFGTPAVGDFNRDGKPDVAASMGGSTSVLLGNGA
                     GGFPTTLSMSAVGGDNLFPYDMNRDGKLDLLEASGHEYRMRLGNGDGTFASPFHVDVA
                     DSVARHPLADMNRDGKQDLIFLSPGFFGGFKVSLGNGTGGFSGIMPASGPLTGYPLDA
                     TAADLDRDGDLDLAVAAEVMAGEGGIRLFSNDGVSSFTYTGTLTSTESYSTITSGDLN
                     RDGAPDLVAYRESPVGFDRGFTGLDVYYNNGAGGFSGAIALGAPERYGAMLEIADVNR
                     DGLADIVCTGDDVADPMSGGAKIATTSVYLQTSPGVFAKRQDHLQGIVGAGFTTVADA
                     DGDRQSDVLGFTKEGFAGYLSILQGNAFAGGWAAAPQQAYAAGAGVIGMAQGDMNRDG
                     IPDLVTANDNESSVSILLGNGSGGFAAPYTFTAPQLSGVQAMEVADINRDGKLDAVTA
                     GGPVCVLFGAGNGTFSSLLQYPGGTLLNDVDVADYNRDGYLDIAATSYANAQIILHLT
                     GPSGSSTTINQLAGYNNNIASADLNRDGKLDLVVANNGNGPIVFRCLRYFLGNGDGTF
                     QGQGLIWSWPTTVQIVYGLALEDVNRDGKVDIVAGLNDQAGTYPIALLYGNGAAGVSS
                     EVDYPTGEQTEYIQIADLDGDPYLDLISSSFGAGYVSVRLGTSSGTFGSRVDVGNATG
                     GAEGLVVADLDRNGTNDLAVADDLNGRVLVLLNTNSNITAVQAAAVPRAAGKLAQNAP
                     NPFNPRTTIRFTLPAAGPTTLTVYDVQGRRVTTLVNQTLEAGDHSMVWEGRDGAGHHL
                     ASGVYFYRLESGGFIATKRMTLLQ"
     gene            complement(3196..3348)
                     /locus_tag="VFM00_05170"
                     /pseudo
     CDS             complement(3196..3348)
                     /locus_tag="VFM00_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3361..3729)
                     /locus_tag="VFM00_05175"
     CDS             complement(3361..3729)
                     /locus_tag="VFM00_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497672.1"
                     /translation="MRALRPAASLVSFAVLILASHPAMAAYPRPLFRAPACLVDVDKP
                     TPAQGEFLQHVTVAEWTGDDLKDLIVTLLGYDGASNAMYDVRFFKNVMATGSPRTFEP
                     VPAPAGFNAPASVVEVLAGD"
     gene            4196..4972
                     /locus_tag="VFM00_05180"
     CDS             4196..4972
                     /locus_tag="VFM00_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HET7497673.1"
                     /translation="MRSPVADRLLSHLGLARESPSLDYLHLIVRAHQLRVPFETLTKL
                     TDYEPGLSRGDFMPPLEVYVDRIVTRGAGGLCWTLARGLKFLLDDLEFDTAFMYMDSG
                     HCCVRVELPEGPYYADVGYAAPLFRAYPLFESFSLDTHREKFVYEVREEGIQVTRNPG
                     PTKRLDPAPRRWEELQAYVEAANDWSAPLSFLHRMSYGRDVNGVYTAFRDGVLTRYVP
                     GGPEKTEVREEDAPAVLADVFGADPELYREALEVRRRYLK"
     gene            complement(4992..5255)
                     /locus_tag="VFM00_05185"
     CDS             complement(4992..5255)
                     /locus_tag="VFM00_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7497674.1"
                     /translation="MGHDRSAATSVVYLFLWRQTAGEDRKRVRMSHREIAEATGLSKS
                     AVQQALRVLHRRGLVHSERRHQTDTPQHAVERPWTRRRQAGEP"
     gene            5247..5474
                     /locus_tag="VFM00_05190"
     CDS             5247..5474
                     /locus_tag="VFM00_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7497675.1"
                     /translation="MAHQIPQALVFVTGNVAGTYETLKAKGVDFAQPPKKERWGESAM
                     FHDSEGNLILFGASERALVNPSRGGLGHERR"
     gene            5489..6145
                     /locus_tag="VFM00_05195"
     CDS             5489..6145
                     /locus_tag="VFM00_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013692837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HET7497676.1"
                     /translation="MRIGILGSGLMGGKLGTIFARAGHEVVFSYARSERKLKRLAREA
                     GGSARAGTPAEAARDADAVLFAVHWSRRNDVLKQAGPMTGKVVLTCSLPMSTDDTRLV
                     MARTSSGAEALAKNLRGARVVSAFGTVPSEVLFSVFASRRRRQRPSLLYCGDDARAKT
                     VAARLIRDVGFEPEDAGPLRMARNLEPFTLVIARLAYEQGKRPELAYRFERFPRRGRP
                     "
     gene            6162..>7428
                     /locus_tag="VFM00_05200"
     CDS             6162..>7428
                     /locus_tag="VFM00_05200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7497677.1"
                     /translation="MKGNEADVVIVGAGASGLEAARMLRARGHSVLVLEARDRIGGRI
                     WTREDPRVPLPIELGAEFIHGETPVTKELLNEAGLSSLEVYARHAEPRRGTLHAMDYW
                     SAINRVLGRVDDDGPDESIAAFLDRKPGGRALARDRSLTRGFVEGFHAADLSRISAQS
                     IAPDPGEPPSAEASRLGRVTSGYGALIAWMARDLRSSLRLNCAVRAVTWRPRRATLDV
                     RRPSGRGLRFTARAVLITVPVGVLKARPGTAGSIAFDPRPPRLDRILSGFEVGSVVRM
                     VVWFREWPWADDDSPRFNFLQLPGEPFQVIWTADPVRWPLAVAWCGGPSAAALSRMPR
                     PEIARRLRAQLAGALRSTPRRLKQAMHTIWFQNWDRDPFARGAYTYVRVSAGDPAPAL
                     ARPEKGTIFFAGEATGAEGGTVEAALESGR"
BASE COUNT         1113 a         2493 c         2551 g         1271 t
ORIGIN      
        1 cgacgtggcc cggcgctccc gatcctgggg cataggtgga agggcgcgtt tctactgaag
       61 cagcgtcatg cgcttcgtgg cgatgaatcc ccccgactcg agccggtaga agtagacgcc
      121 ggacgccagg tgatggcccg cgccgtcgcg accttcccag accatcgagt ggtcgccggc
      181 ttcgagcgtc tgattcacca gggtcgtgac gcgtcgcccc tggacgtcgt agaccgtcag
      241 cgtggtcggc cccgccgccg gcagcgtgaa gcggatggtc gtacgtgggt tgaagggatt
      301 gggcgcgttc tgggcgagct tgccggccgc ccgtggaacg gccgcggctt gcactgccgt
      361 gatattggag ttggtgttga gaagcaccag gactctcccg ttcaggtcgt cggcgacggc
      421 gagatcgttc gtgccgttcc gatccaggtc cgcaacgacg aggccctcgg cgccgccggt
      481 ggcgttcccg acatctaccc tggacccgaa cgtgccggaa gacgttccca gccggaccga
      541 gacgtagccg gccccgaagc tgctagagat caggtcgagg tacggatctc catccaggtc
      601 cgcaatctgt atgtattcgg tctgctcgcc ggtcggatag tccacctccg acgacacccc
      661 tgccgccccg ttcccataga gcagagcgat gggatacgtg ccggcctggt cgttcagccc
      721 cgcgacgatg tcgacctttc catcacggtt gacatcctcg agcgcgagcc cgtagacgat
      781 ctggacggtc gtgggccagg accagatgag cccctgcccc tggaacgtgc cgtcgccatt
      841 ccccaggaag tagcgaaggc accggaagac gatcggaccg ttcccattgt tcgcaaccac
      901 gaggtcgagc ttcccatcac gattgaggtc ggccgaggcg atgttgttgt tgtacccggc
      961 cagctggttg atcgtggtcg aggacccgga cggcccggtc agatggagga tgatctgggc
     1021 gttggcgtag ctcgtggccg cgatgtcgag atatccgtcg cgattgtagt ccgcgacgtc
     1081 cacatcgttg agcagcgtgc cgcccggata ctggagcagg ctgctgaacg tgccgttgcc
     1141 cgccccgaag agaacgcaga ccggcccgcc cgcggtgacc gcgtcgagct tgccgtcccg
     1201 gttgatgtcg gccacctcca tcgcctggac tccagagagc tgaggcgcgg tgaaggtata
     1261 cggcgccgcg aacccgccgc tcccgtttcc cagcaggatg gaaacggagc tttcgttgtc
     1321 gttcgcggtg acgagatcgg gaatgccgtc ccggttcatg tctccctggg ccatgccgat
     1381 aacccccgcc ccggccgcgt aggcctgctg gggcgcggcg gcccatccgc cggcaaacgc
     1441 gtttccctgg agaatggaca ggtagcccgc gaagccctcc ttggtgaacc caaggacgtc
     1501 gctctgccgg tcgccgtcgg catccgccac ggtggtgaat ccggcgccga cgatgccctg
     1561 cagatggtcc tggcgtttgg caaacactcc gggggaggtc tggaggtaga cgctcgtggt
     1621 ggcgatcttg gcgcctccgg acatgggatc cgccacgtca tcacccgtgc acacgatgtc
     1681 ggccagtccg tctcgattga cgtccgcgat ttccagcatg gcgccgtacc gttcgggcgc
     1741 tccaagggca atcgcaccgg agaagccgcc ggctccgttg ttgtagtaga cgtccagtcc
     1801 tgtgaagccg cggtcaaagc cgaccgggct ctcgcggtag gcgaccaggt ccggtgctcc
     1861 gtcccgattc aagtccccgg aggtgatcgt gctgtaggac tcggtcgagg tcagcgttcc
     1921 cgtgtaggtg aaggacgaga ctccatcgtt ggagaagagg cggatgccgc cctcccccgc
     1981 catgacttcg gcggcaacgg ccagatccag gtcaccgtcc cggtccagat ccgccgccgt
     2041 ggcgtcgagc gggtatccgg tgaggggtcc gctcgccggc atgattcccg agaatccgcc
     2101 ggtgccgttc cccaagctca ccttgaaccc gccgaagaac ccaggggaca ggaagatcag
     2161 gtcctgcttc ccgtctcgat tcatgtcggc cagcgggtgc cgcgctacgg aatcggccac
     2221 gtcgacgtgg aagggcgacg cgaacgtgcc gtctccgttc cctagccgca ttcgatattc
     2281 gtggccggat gcctcgagca gatccagttt cccgtcccgg ttcatgtcgt aggggaacag
     2341 gttgtcgccg cccacggccg acatcgacaa cgtcgtcggg aacccgcccg ccccatttcc
     2401 cagcaggacg ctcgtcgacc cgcccatgct cgcggcgacg tccggctttc cgtcccggtt
     2461 gaaatcccca acggccggcg tgccgaacat cgtgggcgcg gggcctgaaa tcacctgatc
     2521 gatccctgta ccggtcccga aggggccaaa gacgacggcc acctggtgcc cggaagaagg
     2581 cacgaccagg tccagctggc cgtcgcggtc caggttcgcg atcaccgggt cgcctccagg
     2641 ccaggggccg gtcgagagcc acctcgtcgg cgcggcgaaa tccaggagct cgaccgtgcc
     2701 gtggggctcg gcttgcccag gcgagagcga ctggtgggtg atgacgagct cggtctgcgc
     2761 gtcggcgtcg agattggcgg gtgtcatgcc gcaggcttga gccttctcca gattccggct
     2821 ggtgccggac gaggagatga ggccgccgcc cgtgccttgg tggatgtcga gggtattgtc
     2881 gtagagaagc accagatccg ggttcccatc gtccgaaatg ttcgcgacgc acgccgcgag
     2941 cggggtcgcc gcggcgggga cggaataggg ggccgcgaag ccggtggccc cgctgctcaa
     3001 gtagaccgtg cattggccgg accctgcccc gacgacgtcc ggcctgccgt cgacgttgag
     3061 atcacccagg cccatgaccg catcggtccc caccgacagg agcggccggg gatcgagcgt
     3121 gccgccgccg gtgtttctca agatggagac atcgtcgatg tgggtcatca cgttccgggt
     3181 gtggacgacc acgtcgaggc gcccgtcctt gtccatgtcg gccagggatg cgcggatgat
     3241 cttcgtcgaa gggccgtaga tataggtcgc tgcggccccc agtcccgatc cggtggagag
     3301 ctgaacccat acggtcgtgg tcgtatcggt catgaccagg tcggcccggc cgtcaccatt
     3361 ctaatcaccg gcaagcacct cgacgaccga tgccggagcg ttgaatccgg cgggcgcggg
     3421 aacgggctcg aaggtgcgcg gacttcccgt ggccatcacg ttcttgaaga agcggacgtc
     3481 gtacatcgcg ttgctggccc cgtcgtatcc caggagggtc acgatgaggt ccttgaggtc
     3541 gtccccggtc cattcggcaa cggtgacgtg ctggaggaac tccccctggg ccggcgtcgg
     3601 cttgtccacg tccacgaggc aggctggggc tcggaacagg ggccgcgggt aggcggccat
     3661 ggccgggtgg gacgcgagga tcaggaccgc gaaggaaact aggctggcag cgggacggag
     3721 ggcgcgcacg atcgttctcc gggtgtgttg gggcctcctg acctcaggtg gacgctatgt
     3781 ccctaacgcg caaatcccgg agcattcggc tcacgccttt tcgtccgcgg gtgtgcccgg
     3841 cggccgggca cggcccatga ggagtgaccc tcgtgggctt ctgtttgacg gttggccgtc
     3901 cggcgaatcc gcgcttgacg caaagtactt tcgctggcga tgcagttcac ccacgaggtg
     3961 acctggaccc tctccgattt cgccatcgcg ggcgccctcc tgttcggcgc cggcctcacc
     4021 ttcgaggcga gcaggggaac caccgccgcg cgcgctgctg tgggtgggat ccggtctctt
     4081 gtttcgaagc gccggcgacg ggggcctacc gaccggaatc gcatcggtga ggtagactgt
     4141 tgcgccatgc tgatgagcgt cgatgtgaat ctggcgctgg ctgggtcctg accccgtgcg
     4201 ctcccccgtc gccgatcgtc tcctgtcgca tctcggcctg gcgcgcgagt ccccctcgct
     4261 cgactatctg cacctaatcg tccgcgccca tcagcttcgc gtgccgttcg aaaccctgac
     4321 caagctcacc gactacgagc ctggtctctc gcgtggagac ttcatgcctc ccctggaggt
     4381 gtacgtcgat cgcatcgtca cgcgcggggc cggcgggctc tgctggacgc tggcgcgcgg
     4441 cctcaagttt ctgctcgatg acctggagtt cgacacggcg ttcatgtaca tggactcggg
     4501 ccactgctgc gtccgggtcg agcttcccga gggcccctac tacgccgatg tgggctatgc
     4561 cgcgccgctc ttccgcgcct atcccctctt cgagtccttc tcgctcgata cccaccgcga
     4621 gaagttcgtg tacgaagtcc gcgaggaggg cattcaggtc acccgcaatc ccggcccgac
     4681 gaaaaggctg gatccggcgc cgagacgatg ggaggaattg caggcatacg tcgaggcggc
     4741 caacgactgg tccgcgcccc tgagcttcct gcaccggatg tcctatggac gcgacgtgaa
     4801 cggggtgtac acggcctttc gcgacggtgt gctcacgcgc tacgtcccgg gcgggcccga
     4861 gaagaccgaa gttcgagagg aggacgcgcc cgcggtgctg gccgatgtct tcggggcgga
     4921 tccagagctg taccgcgagg ctctcgaggt gcgccgccgc tacctgaagt aggtctcaac
     4981 ggcgcgccgg atcacggctc gccggcctgc cgacgcctgg tccacggccg ctcgaccgcg
     5041 tgctgcggcg tgtccgtctg gtgccgccgc tcggagtgca ccagcccgcg ccggtgcagc
     5101 acgcgcagcg cctgctgcac ggcgctcttc gacaggcccg tcgcttccgc gatctcacga
     5161 tggctcatgc ggacgcgctt gcggtcctcc cctgccgtct ggcgccacag gaagagatag
     5221 accacggacg tggccgcgct ccgatcatgg cccaccagat cccgcaggct ctggtgttcg
     5281 tgacgggcaa cgtggccggg acctacgaga cgctcaaggc caaaggggtc gacttcgcgc
     5341 agccgccgaa gaaggagcgc tggggcgaaa gcgccatgtt ccacgacagc gaggggaacc
     5401 ttatcctctt cggagcatcg gaacgggctc tcgtgaatcc ctcccggggc ggactcggtc
     5461 acgagaggcg ctgagcgagg aggagttcat gcggatcggc attttgggtt cggggctgat
     5521 gggcggaaag ctcggaacga tcttcgcgcg ggcggggcac gaggtggtgt tcagctatgc
     5581 ccggagcgag cggaagctga agcgcctcgc tcgggaagcc ggcgggagcg cgcgggccgg
     5641 cacgcccgcc gaggcggcgc gggatgccga cgccgtcctc ttcgcggtgc actggtcgcg
     5701 gcggaacgac gtgctgaagc aggcggggcc catgacgggg aaggtcgtcc tcacctgttc
     5761 ccttcccatg agcacggacg acacgagact ggtcatggcg cgcacctcct cgggggcgga
     5821 ggcgctcgcg aagaacctcc gcggcgcccg cgtcgtctcg gcgttcggca ccgtgccgag
     5881 cgaggtgctg ttctcggtat tcgcctcccg tcgccggcgc cagcgtcctt ccctgctcta
     5941 ctgcggggac gacgcgcgcg ccaagacggt ggccgcgcgc ctcatccgcg acgtcggctt
     6001 cgagcccgag gatgccggtc ccctgcgaat ggcccgcaac ctcgagccgt tcacgttggt
     6061 gatcgcccgg ctggcctacg agcagggcaa gaggccggag ctggcctacc gcttcgagcg
     6121 gttcccgcgc cgcgggcggc cgtgaggggc gtctgacgcg agtgaagggt aacgaagccg
     6181 acgtcgtgat cgtgggcgcg ggcgcgtccg ggctcgaggc cgcacgcatg cttcgtgccc
     6241 ggggccattc ggtgctcgtg ctggaagcac gcgatcgcat cggcggccgg atttggacgc
     6301 gcgaggaccc gcgcgtcccg ctgccgatcg agctcggggc cgagttcatc cacggcgaga
     6361 ccccggtcac gaaggaactc ctaaacgaag ccggattgtc ttccctcgag gtgtacgccc
     6421 ggcacgcgga gccgcggcgc ggaacgctgc acgcgatgga ttactggtcc gcgatcaatc
     6481 gggtcctggg gcgggtcgac gacgacggtc ccgatgagtc catcgccgcc ttcctcgacc
     6541 gaaagcctgg ggggcgtgcg ctcgcccgcg atcgcagtct gacgcggggc ttcgtcgagg
     6601 gcttccacgc ggccgacctc tcacggatca gcgctcagtc gatcgccccc gaccctggcg
     6661 aaccaccctc cgccgaggcg tcgcgcctgg gacgggtgac ctcgggatac ggtgcgctca
     6721 tcgcctggat ggcgcgcgac cttcgttctt cgctccgcct gaactgcgct gtgcgcgccg
     6781 tcacctggcg ccctcggcgc gccactctgg acgtgcgccg cccttccggc cgaggcctcc
     6841 gcttcaccgc gcgcgcggtc ctcatcacgg tcccggtcgg ggtcctcaaa gcgcgcccgg
     6901 ggaccgccgg atcgatcgcg ttcgatccgc gacctccccg actggatcgg atcctgtcgg
     6961 gcttcgaggt ggggtccgtc gttcgcatgg tcgtctggtt ccgcgagtgg ccctgggcgg
     7021 acgacgattc gccccgattt aacttcctgc agctccccgg cgagcccttc caggtgatct
     7081 ggacggcgga tccggtccgc tggccgctcg cggtcgcgtg gtgcggtggt ccttcggcgg
     7141 cggccctttc tcggatgccc cgcccggaga tcgcgcgtcg gctgcgcgcc caactcgcgg
     7201 gcgcgctccg ctcgacgccg cggcgcctga agcaggcgat gcacaccatc tggtttcaga
     7261 actgggatcg cgatcccttc gcacgcggcg cgtacacgta cgtccgagtg agcgccggcg
     7321 atcccgcgcc ggccctggcg cgccccgaga agggcacgat cttcttcgcg ggcgaggcga
     7381 ccggcgccga gggcggtacg gtcgaggccg cgctcgagag cgggcgcc
//
LOCUS       DASSLZ010000136         9545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_47637, whole genome shotgun sequence.
ACCESSION   DASSLZ010000136 DASSLZ010000000
VERSION     DASSLZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9545
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47637"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFM00_05205"
     CDS             complement(<1..533)
                     /locus_tag="VFM00_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HET7497678.1"
                     /translation="MAVLAALAAAFARSYLSHYFGEQISILIFVPAIAFSAWFGGFLP
                     GALTTVLAASAHILMPREVPRSIVVDPRSEEIRLALLLGTGLLISSLYGSLRFVRRGL
                     ERRFDRAQLMLEAARRLSGTLVVEEIHDELRQMIARAIPFDGMIVSSYDHESHVARCV
                     YGWVGGRLLDPSTLPPLT"
     gene            650..949
                     /locus_tag="VFM00_05210"
     CDS             650..949
                     /locus_tag="VFM00_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497679.1"
                     /translation="MNGRKGWTIGIAVLALAGVGAAVMIAGGAVRDFQWHEDARQASL
                     APDQAPGSPSDTGLTRGGPVPRGQAPAVALTVPRFLNAFNAEASDRRLLVLLSPT"
     gene            953..1354
                     /locus_tag="VFM00_05215"
     CDS             953..1354
                     /locus_tag="VFM00_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497680.1"
                     /translation="MCLRGASAVSDILNRHRDWKLRVFVVWEPVRAADSIGPPPGTYT
                     RMRDRRVRQFWDRDRALSQRMVRDIVENPALMPDSERIAEGTVIWDVVALFPPKARWD
                     HAMPKPEWYARPVAEVAPNLEQIVTQLSARR"
     gene            complement(1329..2969)
                     /locus_tag="VFM00_05220"
     CDS             complement(1329..2969)
                     /locus_tag="VFM00_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET7497681.1"
                     /translation="MFKERLAGPAALGLATLLSLMPLACGSPGPSRAAVTHSIQTRVA
                     SAVPTGAIAGERLIEPKAVGRFYKARQSGIAWDHDDVPKIIEAIRGVSNDGLNPDDYH
                     LAAIQKLEGQRSHATTAELEADLDLLLSDAIAAVVDHVRYGRVRPKSLDPRWNVDPRD
                     DMPPLEQTLEEVAKSKDPVNAIQAARPDHFIYKGLMNALAQLREIEASGGWGTVPPGR
                     AIKPGTSDPRVQRVRARLAKSGELDQAASADSTRGFDRALEDAVKLFQARHRLPETGV
                     VDKKTIDAMNVTAAARAAQVRVNLERARWVLGGLKGDFVLVNLPAFKAYYIQGGKNVW
                     EGRTQIGDEAKQTPTFRARMTTVVFNPDWTVPQSIVAQEIFPDIQAGKDGLGSRHLKV
                     YDAKGEEVDPSNVDWGSPDNFPYTLKQPPGDDNALGKVKLLFPNKYSIYMHDTPSKHL
                     FESSSRTFSHGCIRTENVLDLAEILLHGQDGWDHAKIEQTLASGETQNVALENKPYVL
                     IVYWTVTVGASGEVRYADDVYNLDQPLLNALNAGPRAV"
     gene            complement(3153..3719)
                     /gene="pyrE"
                     /locus_tag="VFM00_05225"
     CDS             complement(3153..3719)
                     /gene="pyrE"
                     /locus_tag="VFM00_05225"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET7497682.1"
                     /translation="MTATRDQLLHLLLETGSFKYSDTPTFQLASGAMSRFYVDCRVGL
                     SHAPLRRIVGELMLAEAAGQAVDAAGGLLIGAYPIAIAVSDAAYAAGRTVRAFAVRKE
                     PKSHGLKKLLEGDVKAGDRVLVVDDVITSGKSTIEAIQKCREAGLTVVKAVVMIDRQE
                     QNGRQSIEAEGVPVGALCTLEDLQRIAV"
     gene            complement(3730..6081)
                     /locus_tag="VFM00_05230"
     CDS             complement(3730..6081)
                     /locus_tag="VFM00_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7497683.1"
                     /translation="MALTSGTRIGGYEIAGALGAGGMGEVYRARDTRLDRTVAIKALP
                     DTFAKDPARLARFEREAKLLASLNHPNIAGIHGLVDVDGVPYLILEFVSGETLGARLS
                     REALTVEETLAVGAQIASAIEAAHERGVIHRDLKPGNVMITPAGTVKVLDFGIAKDRS
                     PTGAVSSVAGTEPITAEGAVLGTAAYMSPEQTRGQPVDRRTDVWAFGCILFECLAGKR
                     AFAGRTSSDVMAKVLEREPEWPALPQSVPPKLRDVLKRCLQKEASSRAADIGAIRREL
                     VEMADGSKAPRTGLGTSVPSLAVLYFENLSSDRESDYFCAGITEDILTDLSKIKGLRV
                     ASRNAVARYRGEAADIPRVAAELGVTAVVEGSVRRAGDRVRISAQLINAADGFHLWAE
                     RYDRTLQDVFAVQEEIASSIVAALKVALAPGETEKLGRDRPEDVRAYDLYLKGRERYY
                     LYSRESLHEALDLFQRATQVEPNYALAWAGIADCYGQMIQWDLGPARELTKLGLEAAR
                     RAITINPKLAEGYKAEALVLRGAGDFEASERVLLKAVEVNPRLTAAWINLGVSSYDQT
                     NLAASERRLRRGLETDPQLSYTYGWLNNVLRGTGRHDEALIIGTRGLEVAANAFDRRH
                     AYIAIAAAHLDRQDLPSARKCLLELKEIPGAAAMAQALDAVLATRDGRPEQAGGPLAV
                     AETSLELNPTVLSWAIEACLARGELDRALAFANRPIFLDLHTTILRLTPALQPLLDQP
                     QFGPRVSQRTLVWPAEAPPVPKEIAALFTDVRIESGLPTSTGT"
     gene            complement(6231..>9545)
                     /locus_tag="VFM00_05235"
     CDS             complement(6231..>9545)
                     /locus_tag="VFM00_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET7497684.1"
                     /translation="KINSPTGTSTGPVAVAVGDLNGDGKLDAVTADYGSNTVSVLLGD
                     GSGNLSFTQQNMAGSHPRAVALGDLDGDGFLDVVAANSGSNTISVWLNSGSGIMGART
                     DYATPAEPRGVVIAQFNADSAPDVISCNYLGGTITLFTNQNNGTGGLNVGVSIPVGNK
                     PSAIAAGPLDANTTTDVAVACSARVVTRLSNGAGGFGGGLNLSLPFTPLSIAVQAVTA
                     SSGDIAVAGDGGRGGVNVAYLANVQGASVVGTQSYVNGGATPWGVAFASSSATADLAV
                     ANTGDMAAADPTHTVSVLRRNPFGGFGGMLATTVATTSDRVVSVDVNRDGILDVVVTG
                     ADPNDLVWMQGVGDGTFTNRTTISGGGGTLTWVGTLDANRDAIQDLLFYNPAAAEMRI
                     CLGAGAGFAAPTVAETGYGEPVAIGDLNRDGIPDFAATGGASFRVFLGSGTGSFSVLS
                     PITLVGPTSIRALTLGDWNRDGKLDLAVSTDSGVLRYLGNGDGTFGSATTLLSGHSTR
                     GGAVADLNLDGFPDLAVGEEGVAPLTADENQGVDVLYGNGTGGFGSPSLFPALDVPQM
                     IAIADVNRDGRPDILGKNGYAVPADPQALSVLLGGSLATSADYGAGSNPLGMALGDFN
                     RDGVIDVAGMTGANGGAVNVLRGLPGASFGNLGPRTDYGNVGVLTAGADGADVDGDGD
                     ADFVMVGTISGTPFAGTLSGNGMGGFLGGSVAAITGADPRSCVLADLNRDGRLDLVVG
                     NGNSTNLYVMLGSFSTFGPPLPRATNWPPIRIAVGDLNRDGILDVITSNPTAQDISIL
                     IGDGTGNFAAPTTLTTVNGVTGIAIGDMNRDGLPDIVATGGGTGSARLHLQQPGGVFS
                     MNGQVTIGANPISPVFGDFNRDGWPDVAVGGSGAGGKISVILNDGFGGFSGSAATYSL
                     FAPPTQLRVADMNGDGVQDLIASMPGDVAVLLGAGGPGGGFSSTVHYPAVTAQGLAIL
                     DMNRDGRPDVVAGNSTEQTGSVFLNGGSWPTGVAVGGTPPARPLLSQNAPNPFNPRTE
                     IRFKMPRAGHARVTVFDAAGRRVATLLDGPAPAGERRVEWAGKDDAGHAAASGIYFYK
                     LEADGVSLSRRMALLK"
BASE COUNT         1417 a         3322 c         3326 g         1480 t
ORIGIN      
        1 gtgagcggcg gaagcgtcga ggggtccagc agccgaccgc ccacccagcc atacacgcag
       61 cgcgccacgt gggattcgtg gtcgtaggac gagacaatca tgccgtcgaa ggggatcgcg
      121 cgggcgatca tctggcggag ctcgtcgtgg atctcctcga cgaccagcgt tcccgagagg
      181 cggcgcgcgg cttccagcat gagctgggcg cggtcgaacc gccgctccaa cccgcggcgg
      241 acgaagcgca gggagccgta cagcgagctg atgagaaggc cggtcccgag aaggagcgcc
      301 aggcggatct cctcggagcg gggatccacg acgatcgagc gcgggacttc gcggggcata
      361 aggatgtgcg ccgatgccgc gagcaccgtg gtcaaggcgc ccggaaggaa tccgccgaac
      421 cacgcggaga acgcgatcgc ggggacgaag atgaggatgg agatctgctc gccgaaatag
      481 tgactcagat acgagcgcgc gaacgccgcc gccaatgcgg cgagaacggc cacgaggagc
      541 tcggccagcc aatgtcgtcg aggacggaac acggggcacg agtatagcgc gatcggtgct
      601 aagatcaccc cggcatgggg ggcgggaccg tgaacgctgg ggccatggaa tgaacggccg
      661 caagggatgg acgatcggga tcgcggtgct ggccctcgcg ggcgtcggcg cggcggtcat
      721 gatcgccggt ggcgccgtcc gcgacttcca gtggcacgag gatgcccgtc aggcctccct
      781 ggcgccggat caggccccgg gttccccttc cgacacgggg ctcacgcggg gcggacccgt
      841 gccgcgagga caggcgcccg ccgtcgcact gaccgtgccc cggttcctca acgccttcaa
      901 cgccgaggcc tccgaccggc gcctcctggt cctgctctca cccacctgag ggatgtgcct
      961 gcggggggcc tccgcggtat ccgacattct gaaccgccat cgcgactgga agctccgcgt
     1021 cttcgtcgtc tgggagccgg tccgcgctgc cgattcgatc ggtccgccgc ccggcaccta
     1081 cacgcggatg cgcgaccgcc gcgtgcgcca gttctgggac cgcgacaggg ccctgtcgca
     1141 gcggatggtg cgggacatcg tggagaatcc ggcgctcatg ccggacagcg agcggatcgc
     1201 cgagggaacc gtcatctggg acgtcgtggc cctcttcccg cccaaggcgc ggtgggacca
     1261 cgcgatgccc aagcccgagt ggtacgcgcg gccggtcgcg gaggtcgccc cgaacctgga
     1321 gcagatcgtc acacagctct cggcccggcg ttgagcgcgt tcaggagcgg ctggtccaag
     1381 ttgtagacgt cgtccgcgta gcgcacttcc cccgacgccc cgaccgtcac cgtccagtag
     1441 acgatcagca cgtacggctt gttctccagc gccacgttct gcgtctcgcc gctcgccagc
     1501 gtctgctcga tcttcgcgtg atcccagccg tcctggccgt gaaggaggat ctcggccagg
     1561 tccaggacgt tctccgtgcg gatgcagccg tgactgaagg tccggctgct cgactcgaag
     1621 aggtgcttgc tgggcgtatc gtgcatgtag atcgagtact tgttggggaa caggagcttc
     1681 accttgccga gcgcgttgtc gtcgcccggc ggctgcttca gcgtgtaggg gaagttgtcg
     1741 ggcgaacccc agtccacgtt cgagggatcg acctcctcgc ccttggcgtc gtagaccttg
     1801 agatgccgcg agcccagccc gtccttgccc gcctggatgt cggggaagat ctcctgggcc
     1861 acgatcgact gcggcacggt ccagtcgggg ttgaacacga ccgtggtcat gcgcgcgcgg
     1921 aaggtcggcg tctgcttggc ctcgtcgccg atctgcgtcc tcccctccca gacgttcttt
     1981 cccccctgga tgtagtaggc cttgaacgcg ggcaggttca cgagcacgaa gtcccccttg
     2041 agccccccca gcacccaccg tgcgcgctcg agattcacgc gcacctgcgc ggcgcgcgcg
     2101 gcggcggtca cgttcatcgc gtcgatcgtc ttcttgtcca ccacgcccgt ctcgggcagc
     2161 cggtggcgcg cctggaagag cttgaccgcg tcctccagcg cccggtcgaa gccgcgcgtg
     2221 gagtcggccg aagccgcctg atccagctcc ccgctcttcg cgagccgggc gcgcacgcgc
     2281 tggacccgag gatcggacgt gcccggcttg atcgcgcggc cgggggggac ggtgccccat
     2341 ccgccgctcg cctcgatctc ccggagctgg gccagcgcgt tcatcaggcc cttgtagatg
     2401 aagtgatccg gcctggccgc ctggatcgcg ttcaccggat ccttggactt ggccacttcc
     2461 tccagcgtct gctccagcgg cggcatgtcg tcgcgcgggt ccacgttcca acgtggatcg
     2521 agcgacttgg gccggacccg tccgtagcgg acgtggtcca ccaccgcggc gatcgcgtcg
     2581 gagagaagca gatcgaggtc agcttccagc tccgcggtcg tcgcgtggga gcgctggccc
     2641 tccagcttct ggatcgcggc caggtggtag tcgtcggggt tcagcccatc gttggagacc
     2701 ccgcggatcg cctcgatgat cttgggcacg tcgtcatggt cccaggcgat gccggactgg
     2761 cgcgccttgt agaagcggcc gaccgctttc ggctcgatca gccgctcgcc ggcgatcgcc
     2821 cccgtgggca cggcggaggc gacgcgggtc tggatgctgt gcgtgaccgc ggcgcgggag
     2881 ggacccggcg agccgcaggc cagcggcatc agggaaagca gggtggcaag gccgagtgcg
     2941 gccgggccgg caaggcgctc tttgaacata tgggctccgc ggggcgatgc gccaactccc
     3001 ccaggcgctc tgccccattg gcgttacgtc gtttgcggtg cggcccgatt tcggccggag
     3061 tggcgaacgg gtgaacgcaa ggagcgcgcc atcgcggagg gcacccgatg tgcggctacg
     3121 gagctacggg tcgggcggtc accgcggcaa gctcacacgg cgatgcgctg caggtcctcc
     3181 agagtgcaca gggcgccgac cggtacgccc tcggcttcga tgctctggcg gccgttctgc
     3241 tcctggcggt cgatcatgac gaccgccttc acgacggtca ggcccgcctc gcggcacttc
     3301 tggatcgcct cgatcgtgga cttcccgctc gtgatcacgt cgtccaccac gagcacgcgg
     3361 tcccccgcct tcacgtcccc ttccaggagc ttcttcaagc cgtgcgactt gggctccttc
     3421 cgcacggcga aggcgcgcac ggtgcggccc gccgcgtagg ccgcgtcgga aacggcgatc
     3481 gcgatcggat aggccccgat cagaagcccg cccgcggcgt ccacggcctg acccgcggcc
     3541 tcggccagca tcagctcgcc gacgatccgc cgcagcggcg cgtgcgagag cccgacgcgg
     3601 cagtccacgt agaagcgcga catcgccccc gacgccagct ggaaggtggg cgtgtcgctg
     3661 tacttgaacg atccggtttc aaggagcaga tggagcagct ggtcacgggt cgcggtcatc
     3721 cgatctcctt caggtcccgg tcgaggtggg cagccccgac tcgattcgaa cgtcggtgaa
     3781 caaggcggcg atctcctttg gaaccggcgg cgcctctgcc ggccacacga gcgtccgttg
     3841 ggagacccgc ggcccgaact gcggctgatc cagcaggggc tggagcgccg gagtaaggcg
     3901 caggatggtg gtgtgcaggt ccaggaagat gggtcggttc gcgaacgcga gcgcgcgatc
     3961 cagctccccg cgcgcgaggc aggcctcgat ggcccagctg aggaccgttg gattgagctc
     4021 gagtgaggtc tccgccacgg cgagcggccc ccccgcttgc tccggcctcc cgtctcgggt
     4081 cgcgagcacc gcgtccagag cctgggccat cgcggcggcg cccgggattt ccttcaattc
     4141 caggaggcac ttccgggccg acggcagatc ctggcggtcc agatgcgccg cggcgatcgc
     4201 gatgtacgcg tggcgccggt cgaaggcatt ggcggccacc tcgagcccgc gggtcccgat
     4261 gatcagggct tcgtcatgac gccccgtccc ccggagcacg ttgttcagcc agccgtacgt
     4321 gtagctgagc tgcgggtccg tttccagccc tcgccgcagg cggcgttcgc tcgcggcgag
     4381 gttcgtttgg tcatagctcg agacgccgag attgatccat gccgcggtca ggcgcgggtt
     4441 tacttccacg gccttcaaca acacgcgctc ggaggcctcg aaatcccccg cgcctcgcag
     4501 caccagggct tccgccttgt agccttccgc cagcttcggg ttgatggtga tcgcgcggcg
     4561 cgcagcttcc aggccgagct tggtgagctc ccgggcgggt cccaggtccc attggatcat
     4621 ctgcccatag caatcggcga tccccgccca ggcgagcgcg tagttcggct caacctgcgt
     4681 ggcgcgctgg aacaggtcga gcgcctcgtg cagcgactcc cgcgagtaga ggtaatagcg
     4741 ctcccgcccc ttcaagtaga ggtcgtacgc ccggacgtcc tcggggcggt cgcgtcccag
     4801 cttctcggtc tctcccggcg cgagcgccac cttgagcgcc gcgacgatgg aggaggcgat
     4861 ctcctcctgc actgcgaaga cgtcctgcag cgtacggtcg tagcgctcgg cccagaggtg
     4921 aaagccgtcc gcggcgttga tgagctgcgc cgagatccgc acacggtcgc ctgcgcggcg
     4981 cacgcttccc tccaccacgg ccgtcacccc cagctccgcc gccaccctcg ggatgtccgc
     5041 cgcctcgcct cggtagcgcg cgacggcgtt tcgcgacgca acgcgcagtc ccttgatctt
     5101 ggaaaggtcg gtgaggatgt cctcggtgat ccccgcgcag aagtaatcgc tctcccgatc
     5161 gctggagaga ttctcgaagt agaggacggc gagcgaaggg accgacgtgc cgagaccggt
     5221 gcggggcgcc ttggacccgt cggccatctc caccagctcg cggcggatcg ccccgatatc
     5281 cgcggcgcgc gacgacgcct ccttctgaag gcaccgcttc aggacgtccc gcagtttggg
     5341 gggcaccgat tggggcaggg ccggccactc gggctcgcgc tccaggacct tcgccatcac
     5401 gtccgaggag gtccgcccgg cgaaggcgcg ctttcccgcg aggcattcga agagaatgca
     5461 tccgaacgcc cagacatcgg tgcggcggtc gaccggttgc ccgcgcgtct gctcggggct
     5521 catgtaggcg gcggtcccga gcaccgcgcc ctcggcggtg atcggctcgg tgcccgcgac
     5581 cgacgatacg gccccggtgg ggctgcgatc cttcgcgatt ccgaagtcga gcactttcac
     5641 cgtgccggcc ggcgtgatca tgacgttccc cggcttcagg tcgcgatgga tcaccccccg
     5701 ctcgtgcgcg gcctcgatgg ccgacgcgat ctgcgccccc accgcgagcg tctcttccac
     5761 cgtgagagct tcgcgcgaga gccgcgcgcc cagggtctcc cccgagacga actcgaggat
     5821 caggtagggc acgccgtcca cgtcgacaag gccgtggatg cccgcgatgt tgggatgatt
     5881 gagggaggcg aggagcttgg cctcgcgctc gaaccgcgcg agccgcgccg gatccttcgc
     5941 gaacgtgtcc gggagcgcct tgatcgcgac cgtgcggtcc agccgtgtgt cgcgcgcgcg
     6001 gtagacctcc cccatgccgc ccgcgccgag ggcgccggcg atctcgtagc cgccgattcg
     6061 ggtgccggag gtcagcgcca tggtggtctc gagcgcaagg gtagccgggt cggaaaatgc
     6121 ggggtcatgc cgggaaagat accacgcgcc ttacgggcgc gggagcgcgt gggaacgctg
     6181 agagttcgcc ggcctgctgg ccggcggaca tcggcggaca tcggcgccgc ttacttgagt
     6241 agcgccatcc tccgcgacag cgacacgccg tccgcttcca gcttgtagaa gtagatgccg
     6301 gaagccgccg catggccggc atcgtcctta cccgcccact cgacgcggcg ctcaccggcc
     6361 ggcgccggtc cgtcaagcag cgtggcgacg cgccggcccg cggcgtcgaa caccgtcacg
     6421 cgcgcgtgcc ccgcccgcgg catcttgaag cggatctccg tgcgcggatt gaacgggttg
     6481 ggcgcgttct gcgacaagag cggccgggcc ggcggcgttc cccccacggc cacgccggtc
     6541 ggccacgagc cgccgttcag gaagacggac cctgtctgct ccgtgctgtt ccccgcgacc
     6601 acgtcgggcc ggccgtcgcg attcatgtcc aggatcgcca ggccctgagc cgtcacggcc
     6661 gggtaatgga ccgtcgacga gaaaccgccc ccggggccgc cggcgccgag gagaacggcc
     6721 acgtccccag gcatcgacgc gatcaggtcc tgcacgccgt cgccgttcat gtcggccacg
     6781 cgcagctgcg tcggcggcgc gaacaggcta taggtcgccg cgcttccgga gaagccgccg
     6841 aacccatcgt tcaggatcac cgagatcttc ccgccggctc ccgagccgcc aaccgcgacg
     6901 tccggccagc cgtcccggtt gaagtcgccg aacacggggc tgatcgggtt cgcgccgatg
     6961 gtgacctggc cgttcatcga gaacacccca ccgggctgct gcagatggag gcgggcgctg
     7021 ccggtgccgc cgccggtagc cacgatgtcc gggagcccgt cccggttcat gtcgccgatc
     7081 gcgatgccgg tcacgccgtt cacggtcgtg agggtggtcg gcgccgcgaa gttgcccgtt
     7141 ccgtctccga tcagaatgga gatgtcctgg gcggtcggat tcgacgtgat gacgtcgagg
     7201 atcccgtccc gattgaggtc accgacggcg atgcgaatgg ggggccagtt cgtcgcgcga
     7261 gggagcgggg gcccgaacgt gctgaaggag cccagcatca cgtagaggtt cgtcgagttg
     7321 ccattgccga cgaccaggtc gaggcgcccg tcgcgattca gatccgccag cacgcacgag
     7381 cgcgggtcgg cgcccgtgat cgccgcgacg gagccgccga ggaatccacc catgccgttg
     7441 cccgacagcg tcccggcgaa cggcgttccg gaaatcgtac cgaccatcac gaaatcggcg
     7501 tcgccgtccc cgtcgacgtc ggcgccatcg gctccggcgg tgagcacgcc cacgttccca
     7561 tagtcggtcc gaggccccag gttcccgaag ctcgcgccgg ggaggccgcg caggacgttg
     7621 accgcgcccc cgttcgcgcc ggtcatgccc gccacgtcga tgacgccgtc gcgattgaag
     7681 tcgcccagcg ccatccccag cggattcgaa cccgccccgt agtcggcgct ggtcgcgagg
     7741 ctgcctccga ggagcacgga gagtgcctgc gggtcggcgg gtaccgcgta gccgttcttg
     7801 ccgaggatgt cgggccttcc gtcgcggttc acgtcggcga tcgcgatcat ctggggcacg
     7861 tcgagcgccg ggaacaggga tggcgatcca aagcccccgg tgccgttgcc gtacagaacg
     7921 tcgacgccct ggttctcgtc ggcggtgagc ggcgcgactc cctcctcgcc cacggccagg
     7981 tcggggaacc catccaggtt cagatccgcc acggcgccgc cgcgggtcga gtggccggag
     8041 aggagcgtcg tggcgctccc gaacgtgccg tctccattgc cgaggtagcg caggacgccg
     8101 ctgtcggtgc tgaccgccag gtccagcttg ccgtcccgat tccagtcgcc cagcgtgagc
     8161 gcgcggatcg aggtcggccc cacgagcgtg atcgggctca gcacggagaa cgagcccgtg
     8221 cccgatccga ggaagacccg gaacgacgcg ccgccggtcg ccgcaaaatc cggaatgccg
     8281 tcgcgattca ggtcgccgat cgcgaccggc tcgccgtagc ccgtctcggc cacggtgggc
     8341 gcggcgaaac cggcgccggc gcccaggcag atgcgcatct ccgccgcggc ggggttgtag
     8401 aagagcaggt cctggatcgc gtcgcggttg gcgtccagcg tgccgaccca cgtgagcgtc
     8461 ccgccgccgc ccgagatggt ggtgcggttc gtgaacgtgc cgtcgcccac cccctgcatc
     8521 cagacgaggt cgttcggatc ggcgccggtc accaccacgt ccaggatgcc gtcgcggttc
     8581 acgtccacgc tgacgacgcg gtcggaggtg gtcgcgacgg tcgtcgcgag catgccgccg
     8641 aagccgccga acgggttccg ccgcaggacc gagacggtgt gcgtcggatc ggccgcggcc
     8701 atgtcgcccg tgttcgcgac cgcgagatcc gcggtcgctg aagacgacgc gaaggcgacc
     8761 ccccagggcg tcgctcctcc attcacgtag gactgggttc ccaccacgga cgcgccctgg
     8821 acgttggcga ggtaggccac gttcacgcca ccccggccgc cgtccccggc gaccgcgatg
     8881 tcgcccgagc tcgcggtgac cgcctggacc gcgatcgaaa gcggagtgaa gggcagcgac
     8941 aggttgagac cgccgccgaa tcctccagcg ccgttgctca gtcgcgtcac cacgcgcgcg
     9001 gagcaggcga cggcgacgtc ggtggtggtg ttggcatcca ggggacccgc ggcgatcgcg
     9061 ctcggcttgt tccccaccgg gatgctcacg cccacattca ggccgccggt gccgttgttc
     9121 tggttcgtga agagggtgat cgtgccgccg aggtagttgc acgagatcac gtcgggcgcc
     9181 gagtcggcgt tgaactgcgc gatcaccacg ccgcgcggct ccgccggcgt ggcgtagtcg
     9241 gtgcgggcgc ccatgatgcc ggagccgctg ttgagccaga ccgagatcgt gttcgatccc
     9301 gagttggccg cgaccacgtc gaggaacccg tcgccgtcca gatcgccgag ggcgaccgcg
     9361 cgcgggtgcg agccggccat gttctgctga gtgaaggaga ggttgcccga gccatcgccc
     9421 aggagtaccg acaccgtgtt gctgccgtaa tcggcggtga ccgcgtccag tttcccgtcg
     9481 ccgttcaggt cgccgacggc aaccgcgacc ggaccggttg aggtccccgt gggggagttg
     9541 atctt
//
LOCUS       DASSLZ010000137         3434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1185966, whole genome shotgun sequence.
ACCESSION   DASSLZ010000137 DASSLZ010000000
VERSION     DASSLZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185966"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFM00_05240"
     CDS             <1..489
                     /locus_tag="VFM00_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018975103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7497685.1"
                     /translation="RAADGSVTMTVDVLDLQALLHESSAAEGDLRSVTPESLPPAPAA
                     AAIGHVHLHVADLDAARRFYADVLGFDVVTRYGAEALFVSAGGYHHHVGLNTWAGKGA
                     SPPPPGAAGLRWFAIEHPSAAEREETVSRVREAGIDVETIGSDYLVRDPSRNGIRLTA
                     RS"
     gene            complement(473..1978)
                     /locus_tag="VFM00_05245"
     CDS             complement(473..1978)
                     /locus_tag="VFM00_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7497686.1"
                     /translation="MTTRRGLRILLLLLASHLGTAPTPARALVYTRHPWLSLQTSTSM
                     LVAWQTDVASSGRVLYGVDPVTNWSTALQATQPGTVADHAVALSGLSPATRYRYKVVS
                     NTDTLDAGAFRTAPVAAEPFRFLAFGDLGTATADQRLIAARADTLNADLAILTGDIIY
                     EQGEPFNFTPYYFTIYRPTLKRVPFYPVLGNHDTYYDGGASFVSAFYLPQNFASGPER
                     SYSFDYSNAHFAAIEVVNENVAPPAAAVSWLDADLAASTKPWKFVFFHVPMYSNGGGH
                     GGDVAIAASLEPIFLARGVDMVFQGHNHFYTRTYPIVSGTAVDQVQEPTYFHPHGPVY
                     VVAGGGGRALYALTAPAPYEALSRSIFHLVSVDVAGDSLTLRAIQEDGTVFDRMTLRK
                     GTTTHAEDPPADPSAPAPRFAIQRARPNPASGAADLRFTLAGPSAATLRILDAAGRRV
                     RTVDLGELGAGEHAWRWDGRDDRGRPVASGRYFVEVRAGARRARTPLTIVR"
     gene            complement(2029..2383)
                     /gene="ssrA"
                     /locus_tag="VFM00_05250"
     tmRNA           complement(2029..2383)
                     /gene="ssrA"
                     /locus_tag="VFM00_05250"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2670..3203
                     /locus_tag="VFM00_05255"
     CDS             2670..3203
                     /locus_tag="VFM00_05255"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET7497687.1"
                     /translation="MRLTHFRMRLAGVLGAAAILALSAVTAQAQTRYDRQTTREVLSR
                     MHHVNQMEIRLGALAMTKGRDPDVRDFGRQLRDDHIRNDRQVVNLASREGVQLVAIGM
                     NPHERQVSRRLRMARGHEFDREFLDAMAQGHTQVIQMMRQAHRDVRDPEVRRFIGSTL
                     PALREHRNMARSFQDQL"
BASE COUNT          564 a         1119 c         1217 g          534 t
ORIGIN      
        1 cgcgccgccg acggatcggt caccatgacg gtcgacgtgc tggatctcca ggcactgctc
       61 cacgagtcgt cggcggcgga gggtgatctt cgtagcgtca cgccggaatc gctcccgccc
      121 gcgccggcgg cggccgccat cggccacgtc catctccacg tcgccgatct cgacgcggcc
      181 aggcgcttct atgccgacgt gctgggattc gacgtcgtga cgcgctacgg cgccgaagcg
      241 ctcttcgtct cagcgggcgg ataccatcat cacgtggggc tgaatacgtg ggccgggaag
      301 ggcgcctctc cgccgccgcc gggagcggcc gggctccgct ggttcgcgat cgagcacccg
      361 agcgccgccg agcgggaaga gaccgtgtcg cgcgtccgcg aggcggggat cgacgtcgag
      421 acgatcgggt cggactacct ggtgcgcgat ccctcgcgaa acgggatccg gctcaccgca
      481 cgatcgtgag cggcgtgcgg gcccgccgcg cgcccgcgcg cacctcgacg aaatagcgcc
      541 ccgaggcgac cggacgcccg cgatcgtcgc gtccgtccca acgccacgcg tgctcgcccg
      601 cgccaagctc gcccagatcg accgtgcgga cgcgacggcc cgccgcatcc aggatccgga
      661 gcgtggcggc cgatgggccg gccagcgtga agcgcagatc ggcggcgccg gacgcggggt
      721 tggggcgcgc cctctggatc gcgaaccggg gtgccggggc ggacgggtcg gcgggcgggt
      781 cctccgcgtg cgtggtcgtt cccttccgca gcgtcatcct atcgaagacg gtcccgtcct
      841 cctggatcgc gcgcagcgtg agcgagtccc ccgccacgtc cacggacacg agatggaaga
      901 tgctgcgcga caaggcctcg tagggcgccg gcgcggtgag cgcgtagagc gcgcggccgc
      961 cgcctccggc cacgacgtag accggtccgt gcggatggaa gtaggtgggc tcctggacct
     1021 ggtccaccgc ggtccccgag acgatgggat aggttcgcgt gtagaagtgg ttgtgcccct
     1081 ggaagaccat gtccacgccg cgcgcgagga agatcggctc gagcgacgcg gcgatggcca
     1141 catcccctcc gtgcccgcct ccgttcgagt acatcggcac gtggaagaag acgaacttcc
     1201 agggcttcgt gctcgcggcg aggtcggcgt ccagccagga gaccgcggcc gccggaggcg
     1261 cgacgttctc gttcaccacc tcgatcgccg cgaagtgcgc gttggagtag tcgaaggagt
     1321 agctgcgctc gggcccggag gcgaagttct gcggcaggta gaaggcgctc acgaaggacg
     1381 cgccgccgtc gtagtaggtg tcgtgattgc cgagaacggg atagaagggg acgcgcttca
     1441 gggtcgggcg gtagatggtg aagtagtagg gggtgaaatt gaagggttcc ccctgctcgt
     1501 agatgatgtc gcccgtgagg atcgcgagat cggcgttcag cgtgtcggcg cgcgccgcga
     1561 tcagccgctg gtccgcggtc gcggtgccca gatcgccgaa cgcgaggaag cggaagggct
     1621 ccgccgcgac gggcgccgtc cggaacgcgc ccgcgtcgag cgtgtcggtg ttcgaaacga
     1681 ctttgtagcg gtagcgcgtc gccggcgaaa ggccgctgag cgcgacggcg tggtcggcga
     1741 cggtaccagg ctgcgtggcc tggagcgcgg tgctccagtt ggtcacggga tcgacgccgt
     1801 agaggaccct gcccgaggag gcgacgtcgg tctgccaggc caccagcatc gaggtcgagg
     1861 tctggagcga gagccacggg tggcgcgtgt agaccagcgc gcgcgccggc gtcggcgcgg
     1921 taccgaggtg cgacgccagg agcaggagca ggatccggag gccacgccgc gtcgtcatgg
     1981 acgaaggata aacaaaaccc cggccgcggg cgaccggggt cgagcgattg gtggaggcgg
     2041 agggaattga accctcgtcc gaaagcggct cctgtaggac cagctacgcg cgtagtccgc
     2101 tgtttcagcc ttcgcggtcc cggacacaag cggacaaggt cacgttaccg ctatcccgat
     2161 agagtctcgc cctctcgact caggcggatc tcgagggcca gcccaccttc atgacgctcg
     2221 ttccggcctc ggcaggcgca gaccgggcga gcgcaagcgg cttagttagg ccgcgagagc
     2281 caattcagta ttggcttctg tgttttgccg gttgttttac gaggcctccg gcacctcggc
     2341 gcgcgttcct agattcacca ctccgtcgaa tccattcgcc cccataggtt tgtcatctgg
     2401 agccgccgtg agtgtaccac ctgaggatcg gccgctcccg ccaaccccat tagatgcaat
     2461 cgggcgagag tgcgcttcgg gcggggcgac ctgtgttgcc ccttttgcgg cactgttccc
     2521 gcctgtccta cacgcctcgg ccggggctcc ccgccgatcc ccgccaagtg actgctggaa
     2581 ggtaggttaa gtgatcaggc gacaggcacc tcactggcct tcaacttgcg tccaagagca
     2641 tgggaccggc tcaggaaagg agacccatca tgagactcac ccattttcga atgcgactcg
     2701 ccggcgtcct gggagccgcc gcgatcctgg ccctgtcggc cgtcacggcg caggcgcaga
     2761 cccgctacga ccggcagacc acccgcgagg tgctctcccg gatgcaccac gtgaaccaga
     2821 tggaaatcag gctgggcgcg ctcgcgatga ccaagggacg cgacccggat gtccgtgact
     2881 tcggcaggca gcttcgggat gaccatatcc ggaacgaccg gcaggtcgtg aacctcgctt
     2941 cgcgcgaggg cgtgcagctt gtcgccatag gtatgaatcc gcacgagcgt caggtgtcgc
     3001 ggcggcttcg gatggcgagg gggcatgaat tcgaccgcga gttcctggat gccatggctc
     3061 agggtcacac gcaggtcatc cagatgatga ggcaggcgca tcgcgacgtt cgcgaccccg
     3121 aggtgcgccg gttcatcgga agcacactgc ccgcgctacg cgagcaccgg aacatggcgc
     3181 ggagtttcca ggatcagctg tagcaggtag cgatcggcag catgacccat cccgagtcgc
     3241 gtccgcctcg gcaaggcggg cgcgctcggg ggctgctgcc tgatgagtct tcgcgcttcg
     3301 ctcatggacc cgctctcgcc tagtcttatg cgatgaattc gggcgcggcc tcatgaacga
     3361 agcggctccg gagactccgg gcaggatcca ccgggcgatc gacacgaagg cgttcgagca
     3421 tccgccgatg cgaa
//
LOCUS       DASSLZ010000138         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_804933, whole genome shotgun sequence.
ACCESSION   DASSLZ010000138 DASSLZ010000000
VERSION     DASSLZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804933"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFM00_05260"
     CDS             <1..290
                     /locus_tag="VFM00_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497688.1"
                     /translation="RSLAFLYVALLLHSIVIPFITVPRAVILQRVVPPARMGRVFSLV
                     NFTVFGVTAISVAIAGAVLDRITAPQMYAITGFCGGLTGILGMASRRLRAL"
     gene            complement(328..1713)
                     /locus_tag="VFM00_05265"
     CDS             complement(328..1713)
                     /locus_tag="VFM00_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7497689.1"
                     /translation="MSAQATPAPARAGHADALLRRQIGLYAATAVTVGSIVGSGIFRS
                     PHSVAQELHSVPVMLFAWVLGGVLSMCGSLVLAELAVAHPRTGGLYVFIREAFGDRLG
                     FVFGWASLSVIKPTVIASITTVFAIYFCHALGLAEAVRLPVGLAAIFILTLINWLGVK
                     QGAGTQTLLTTLKVIGILGLCAAAFFLPHGGAAAGEASTAAATHPAPHPLLIALAISM
                     IPIFFAYDGWTDSTYVAGEIKNPRRAMPIAILVGTMIVIAVYVITNLAYFHVLSPAEV
                     AHYEAVASEAIRRILGEGGSRALAVLVAVSTFGTISASVLTGPRVTLAMAADGLFWRR
                     AAHVSSRGTPDFALWLQALLSGIWLWCANGFEDVSGWFVTTSWLFYGLTTAALFVRRR
                     QGAAAARDAEAGDGTGYRTPLYPVTPILFILVTLAIIASDLVSSGWRSAAGVAIAATG
                     FPLYALMSRKR"
     gene            1842..>2252
                     /locus_tag="VFM00_05270"
     CDS             1842..>2252
                     /locus_tag="VFM00_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7497690.1"
                     /translation="MNATRTRLDPRTSIGAVDVAVSDLDRSIRFYKRALGFQVHAREG
                     EHARLGAGGRDLLRLEEVRGARRVPRTTGLYHFAVLLPGRTDLATALVRLAASGAPLG
                     GASDHGVSEALYLSDPDGNGIEIYRDRPREEWPRA"
BASE COUNT          355 a          781 c          810 g          306 t
ORIGIN      
        1 gccgatcgct cgcattcctc tacgtggcgc tgctcctcca ctccatcgtg atccccttca
       61 tcaccgtgcc ccgcgcggtg atcctccagc gcgtcgtccc gcccgcgcgc atggggcgcg
      121 tcttctcgct cgtcaacttc accgtgttcg gggtgaccgc gatctcggtc gcgatcgccg
      181 gagccgtgct ggaccggatc acggcgccgc agatgtacgc catcacggga ttctgcggcg
      241 ggctgacggg aattttgggc atggcgtcgc gcaggctgcg cgcgctctag ccgaaggggc
      301 gatggccgga cgcgacccgg cgccgcgtca gcgcttccgg ctcatcaggg cgtagagcgg
      361 gaaccccgtc gccgcgatgg ccacgcccgc ggccgaccgc catccgctcg acaccaggtc
      421 gctcgcgatg atcgccaggg tgacgaggat gaagaggatc ggagtcaccg ggtagagcgg
      481 ggtgcggtat cccgtcccgt ctccggcctc ggcgtccctg gcggccgccg cgccctgccg
      541 ccggcgcacg aagagcgcgg cggtcgtgag cccgtagaag agccaggagg tggtgacgaa
      601 ccatcccgag acgtcctcga acccgttcgc gcaccagagc cagattcccg agagtagggc
      661 ctggagccag agcgcgaaat cgggcgtgcc gcgactcgag acgtgagccg cgcggcgcca
      721 gaagagccca tcggcggcca tcgcgagcgt gacgcgcgga cccgtgagca ccgacgccga
      781 gatcgtcccg aacgtggaga ccgcgaccag cacggccagc gcgcggctgc cgccctcgcc
      841 caggatgcgc cggatcgcct cgctggccac cgcctcgtag tgggccacct cggcgggcga
      901 gagcacgtga aagtaggcga ggttggtgat gacgtagacg gcgatcacga tcatcgtgcc
      961 gaccaggatc gcgatcggca tcgcgcggcg ggggttcttg atctcgcccg ccacgtacgt
     1021 cgagtcggtc cagccgtcgt aggcgaaaaa gatcggaatc atggagatcg cgagcgcgat
     1081 caggagcgga tgcggcgcgg gatgcgtcgc cgcggcggtg gacgcctctc cggcggcggc
     1141 gccgccgtgc ggcaggaaaa acgcggcggc gcagaggccc aggatcccga tcaccttgag
     1201 cgtggtgagg agcgtctggg tgcccgctcc ctgcttcacc cccagccagt tgatcagcgt
     1261 gaggatgaag atcgcggcga gccccacggg aagacggacc gcctcggcga ggccgagcgc
     1321 atggcagaag tagatggcga agaccgtcgt gatcgaggcg atgacggtcg gcttgatgac
     1381 ggagagggag gcccagccga agacgaagcc caggcggtcg ccgaacgcct cgcggatgaa
     1441 gacgtagagt cccccggtgc gggggtgcgc caccgccagc tcggccagca cgagcgaccc
     1501 gcacatcgag agcacgccgc cgagcaccca ggcgaacagc atgacgggga ccgagtggag
     1561 ctcctgcgcg accgagtgcg gggagcggaa gattcccgag ccgacgatgc ttccgacggt
     1621 caccgccgtg gcggcgtaga gtccgatctg gcggcggagc agcgcgtccg cgtggcccgc
     1681 gcgcgcgggc gcgggggtgg cttgggcgct catgatcctc cctcctggtg ggaatcccgc
     1741 cggaggctcg aggcatccga gcaaccgtcg agcgataccc tcgtccgccg ccccttatac
     1801 cacggcccgg gccggccggg tgttcgccgc aggaggaatg gatgaacgcg acccgtaccc
     1861 ggttggaccc cagaacctcg atcggcgccg tggacgttgc ggtttccgat ctggaccgct
     1921 cgatccgctt ctataagcgc gccttgggat tccaggtcca tgcccgcgaa ggcgaacacg
     1981 cgcgactggg cgcgggcggc cgcgacctgc tccgcctgga agaggtccgc ggcgcgcggc
     2041 gcgtcccgcg caccaccggc ctctaccact tcgccgtgct cctgccgggc cgtaccgacc
     2101 tggcgacggc gctcgtccgc ctggccgctt cgggcgcgcc gctcggcggc gcttcggatc
     2161 acggcgtgag cgaggctctc tacctgtccg atccggacgg taacggcatc gagatctacc
     2221 gcgaccgccc gcgcgaagag tggccgcgcg cc
//
LOCUS       DASSLZ010000139         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1186127, whole genome shotgun sequence.
ACCESSION   DASSLZ010000139 DASSLZ010000000
VERSION     DASSLZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186127"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1218
                     /locus_tag="VFM00_05275"
     CDS             40..1218
                     /locus_tag="VFM00_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HET7497691.1"
                     /translation="MSAFEPPLLARHYTRFRVAERLLLTGHSHQAWPDVAFDGQLEAW
                     NDAAELVDEKWGRAADKAERVRRGFARLLGGAPGEIALGQNTHELVLRFLSALPLVDR
                     PRLVSTDGEFHSIRRQLDRLAEDGRLEIVKVPAGPPDAIAERLAAAVNERTSAVLASS
                     VLFGNAAIVPGLGSVAAACRRAGAELLVDAYHHLNVVPFSLRGEALEEAFVVGGGYKY
                     CQLGEGNCFLRVPPHAREYRPVITGWYGEFAHLTDRADARVSYAEGPERWAGATYDPT
                     SHYRAARVFDFFEDQGLTPERLRAISEHQIRLLADGFDALGLDPGVIARDRSIPLERI
                     GGFLALRSPGAEAICAGLRALGVLADVRGDLLRLGPAPYLADEQIERAIAELGRVARA
                     "
     gene            1316..1882
                     /locus_tag="VFM00_05280"
     CDS             1316..1882
                     /locus_tag="VFM00_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7497692.1"
                     /translation="MDGTAILLQRIRDGDERAREELARAYLPVARRLAHGRLPASARG
                     LSETDDIASRSVTQAVSHAARFEMRHEAAFLVYLRRIVINEIRTEIRRAKARPSGGEL
                     DTDVAWNGPSPLDNVIGRELMNRYERALDQLTPHQREAVVLRLEFRLGYREIAAKMDL
                     DSPDAARMLVSRAMVRLAKLIAPQDGPS"
     gene            1879..>2249
                     /locus_tag="VFM00_05285"
     CDS             1879..>2249
                     /locus_tag="VFM00_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017606661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497693.1"
                     /translation="MSEPRDDEPIDEERLRFAERIVDRTPIDWDSDAAGAGLSPDETS
                     GFQELERLVAAHVRVAKEFDEINAPGAGSLISHYRVVSRLGSGGMGVVYQAQDERLDL
                     GRLVALKLVHPEASGDDVMRQV"
BASE COUNT          321 a          818 c          776 g          334 t
ORIGIN      
        1 gcgtcggcga agccgacgcc aagcgagggc cagacgagcg tgagcgcttt cgaaccgccg
       61 ttgctggccc ggcactacac ccgcttccgc gtcgccgagc ggctgctcct cacgggccat
      121 tcgcaccagg cctggcccga cgttgctttc gacggccagc ttgaggcatg gaacgacgcg
      181 gcggaactcg tggacgagaa gtggggacgg gcggcggaca aggccgagcg cgtgcgccgc
      241 ggcttcgcca ggctcttggg cggcgcgccc ggcgagatcg ctctcgggca gaacacccac
      301 gagctggtgc tgcgctttct ctccgcgctc cccctggtcg atcgaccgag gctggtcagc
      361 accgacggcg agttccactc gatccgccgc cagctcgacc ggctcgccga ggacgggcgg
      421 ctcgagatcg tgaaggttcc cgccggccct ccggacgcca tcgccgagcg cctcgccgcc
      481 gccgtgaatg aacgcacgtc cgccgtgctc gcctcctccg tgctcttcgg aaacgcggcg
      541 atcgtcccgg ggctggggtc cgtggccgcg gcgtgccggc gcgccggagc cgagctcctg
      601 gtcgacgcct accaccacct gaacgtcgtg cccttttccc tgcgtgggga ggcgctggag
      661 gaggcgttcg tcgtgggcgg aggctacaag tactgccagc tgggagaggg gaactgcttc
      721 ctccgcgttc ccccgcacgc ccgcgaatac cgccccgtca tcaccggctg gtacggcgag
      781 ttcgcgcacc tcaccgatcg cgcggacgcg cgcgtgtcct acgccgaggg gccggaacgc
      841 tgggcgggcg ccacctacga cccgaccagc cactaccgcg cggcacgcgt cttcgatttc
      901 ttcgaggatc aggggctcac gccggaacgg ctgcgcgcga tcagcgagca tcagatccgc
      961 ctgcttgcgg atggcttcga cgcgctcggt ctcgatcccg gggtgatcgc ccgcgaccgc
     1021 tcgatccctc tcgaacggat cggcggattc ctcgcgctcc ggtctcctgg ggccgaagcg
     1081 atctgtgccg gtctcagggc tctcggcgtc ctggccgatg ttcggggcga cctgctccgc
     1141 ctgggccccg cgccctacct ggcggacgag cagatcgagc gcgcgatcgc ggagctgggc
     1201 cgggtcgccc gcgcctagcc gcattcgggc ccctctggac gagcccatcc ccggagcccc
     1261 atacttcgtt cgacaccccc cacaactgtc tcccaaggga agcgagggcc cccgcgtgga
     1321 cggcaccgcg atcctccttc agcgcatccg cgatggcgac gagcgcgcca gggaagagct
     1381 cgcccgggcc tacctgccgg tcgcccggcg cctggcgcac ggacgcctgc ccgcgagcgc
     1441 gcggggcctc tccgagaccg acgacatcgc gtcccgctcg gtcacccagg ccgtgagcca
     1501 cgcggcgcgc ttcgagatgc gccatgaagc ggcgttcctc gtctacctcc gccgcatcgt
     1561 catcaatgag atccgcaccg agatccgacg ggcgaaggcg cgcccctccg gcggcgagct
     1621 ggataccgat gtcgcctgga acgggccttc gccgctggac aacgtgatcg ggcgggagct
     1681 gatgaaccgc tacgagcgcg ctctggacca gctcacgcct caccagcgcg aagccgtcgt
     1741 cctgaggctg gagttccggc tgggataccg ggagatcgcc gccaagatgg atctcgactc
     1801 gcccgacgcg gcccggatgc tggtgtcccg ggccatggtc cggctggcga agctgatcgc
     1861 tccccaggac ggtccttcat gagcgaaccg cgcgacgacg agccgatcga cgaagagcgg
     1921 cttcgattcg cggagcggat cgtcgaccgg accccgatcg actgggactc cgacgcggcc
     1981 ggcgccgggc tctcgccgga cgagacgagc ggctttcagg aactggagcg gctggtggcg
     2041 gcgcatgtcc gggtcgcgaa ggagttcgac gagatcaacg cgcccggcgc cggatccctc
     2101 atttcccact atcgcgtcgt gagccgcctc ggctcggggg gcatgggggt cgtctatcag
     2161 gcccaggacg agcggctgga cctgggccgt ctcgtcgcgc tgaagctggt tcatccggaa
     2221 gcctccggcg acgacgtgat gcgccaggt
//
LOCUS       DASSLZ010000140         3959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_680903, whole genome shotgun sequence.
ACCESSION   DASSLZ010000140 DASSLZ010000000
VERSION     DASSLZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680903"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFM00_05290"
     CDS             complement(<1..621)
                     /locus_tag="VFM00_05290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958372.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET7497694.1"
                     /translation="MPATTTPLPNTSETAPEFKTFTINMGPQHPSTHGVLRLIIELDG
                     ETVVSLKPVMGYLHTGMEKIMESKTYTKSITVTDRMDYLSPLGNNMAYVGAVEKILGT
                     EVPDRAKTLRIIFLEIMRISSHLVWLGTHALDLGAMSVFLYCFREREKLLDLNDMCAG
                     SRLTPSYFRVGGFFQDVPDAFVPAVRKFLTEFPAALAEYQNLLTKNR"
     gene            complement(751..1200)
                     /locus_tag="VFM00_05295"
                     /pseudo
     CDS             complement(751..1200)
                     /locus_tag="VFM00_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(1296..2060)
                     /locus_tag="VFM00_05300"
     CDS             complement(1296..2060)
                     /locus_tag="VFM00_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET7497695.1"
                     /translation="MTAGPAWTAIACALVAGGANVFGGLLAAKASGWDRIRQSYFVAI
                     GAGFMLAAVLLRMVPESYRLALKGETAMLSVGTLILVGYLLVHFAEHVLVAHFHFGEE
                     THHEHWVEPVVGTTALMGLALHTLFDGISIGSGFLIDPGLGLLIAIAVFLHKIPEGFT
                     MASIMVAAGRSRRDAILAATFLGVMTVVGTLGTTMLRGSVRIALPVSAGAAIYVAASD
                     LIPAVNEAKGIKKPLGVFAGVLLFYATEEILGHLGL"
     gene            complement(2091..2531)
                     /gene="nuoI"
                     /locus_tag="VFM00_05305"
     CDS             complement(2091..2531)
                     /gene="nuoI"
                     /locus_tag="VFM00_05305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET7497696.1"
                     /translation="MKGLSITLSHFFKKPVTLKYPDQRMTMFPRFRGLHELHRYSNGL
                     ERCVCCGLCAAVCPADAIYMEAGENSVTERFSAGERYAKRYEIDMLRCIFCGYCEEAC
                     PEDAIFLGHNFELSDYSRDSFIYAKEQLLVPVGTTHSPGIVRGR"
     gene            complement(2624..>3959)
                     /locus_tag="VFM00_05310"
     CDS             complement(2624..>3959)
                     /locus_tag="VFM00_05310"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7497697.1"
                     /translation="DRFEEASTHRVMAMAHHLLGHREKALRQAQEGVSLLRKLEAVYE
                     LTRLLVWLGETLLAGGDAEERRAARDHLWEARSHAMSMRLDGWVERIEKVLGVDLRPA
                     APAPARRNAADPMPEGADPDCFRFGMVTQDARVRELLRVLERASLSRLPILILGESGV
                     GKELLARAAHALSDRRGEIFVVGHCAALPEGQLDADLFGEELGEVSGHRGSRPGLFEA
                     ANRGVIFLDEVSELLTSAQAKLLRVIETGELRRLGGTGLTQVDVRVIAASTRDVAALV
                     RRGLFRDDLYYRLNGIRLEVPPLRERPDDVLLIGQYLLDRASAAAGKRVRLTPDAWKL
                     LTTHSWPGNVRELRNVMDRVAALATDGEEIDAAAIQLQPATRPGSRGGARRAATRAGS
                     AEREVILHALRSHGGNQSEAARSLGGMKRTTLLYKIKKLDIRPEEYGRSPEA"
BASE COUNT          627 a         1318 c         1392 g          622 t
ORIGIN      
        1 ccggttcttc gtgaggaggt tctggtactc ggccagcgcc gcgggaaact ccgtgaggaa
       61 cttccggacg gcgggcacga acgcgtcggg cacgtcctgg aagaacccgc ccacgcggaa
      121 gtacgacggc gtcaggcgcg atcccgcgca catgtcgttc agatcgagga gcttctcccg
      181 ctcgcggaag cagtagagga agacgctcat cgcgccgagg tccagggcgt gcgtccccag
      241 ccagaccagg tgcgaggaga tccgcatgat ctcgaggaag atgatccgga gcgtcttggc
      301 gcggtcgggt acctcggtgc ccaggatctt ctccaccgcg ccgacgtacg ccatgttgtt
      361 ccccagcggg gacaggtagt ccatccggtc ggtgaccgtg atcgacttgg tgtacgtctt
      421 cgactccatg atcttttcca tgcccgtgtg gagatagccc atcaccggct tcaggctcac
      481 gaccgtctcg ccgtccagct cgatgatcag gcgcagcacg ccgtgggtgg acgggtgctg
      541 cggacccatg ttgatcgtga aggtcttgaa ttcgggcgcg gtctcgctgg tgttgggcag
      601 gggcgtcgtg gtggcgggca tgacgttcta ggcggccgtc tccggcggcg tgttggtcgc
      661 ggcggcgcgg ccgatctcat ccgacgccgg agcctccgcg accgcgacgt cgtggtcgcc
      721 gggcaccggg gcgtggagct tcatccggtc gatcttttcc tggagcttca tgatgccgta
      781 gatcagagcc tcgggacgcg gcgggcagcc gggcacgtag acgtccaccg gcaccacggt
      841 gtccaccccc tgcacgatgg cgtaatcgta gaactcgccg ccgcacgagg cgcaggcccc
      901 catggagatc acccacttcg gctcgggcat ctggtcgtag atgcgtttca ggatcggagc
      961 catcttcttg ctgacccggc cggccacgat catgaggtcg gcctggcggg gcgaggcgcg
     1021 gaacacctcg gccccgaagc gggcgaggtc gtagcgggag gcgctggtcg ccatcatctc
     1081 gatggcgcag caggccagcc cgaaggtggc gggccagagc gacgaacggc gggcccagtt
     1141 caccatcttc tcgacggtgg tcgtgaggat gccgtccgga atcagttctt caagtcccat
     1201 ggatcacctg ttcggtcagt tcgtgaaaaa gggcacgatg ctcagcgcaa gtccctttgg
     1261 aaccgtacac tagcagcggc aacgggttgc gtcaatcaca accccaggtg cccgaggatc
     1321 tcctcggtcg cgtagaagag gagcacgccc gcgaagaccc ccaatggctt tttgatgccc
     1381 ttggcttcgt tcacggcggg gattaaatcg gaggcggcga catagatggc cgctcccgcc
     1441 gagaccggaa gcgcgatccg caccgacccc cgcagcatgg tcgtccccag cgttcccacc
     1501 accgtcatca cgcccaggaa ggtcgccgcc aggatcgcgt cccggcggga gcggcccgcg
     1561 gcgaccatga tcgacgccat ggtgaagccc tcggggatct tatggaggaa gacggcgatg
     1621 gcgatgagga gccccaggcc gggatcgatc aggaatcccg agccgatcga gatcccgtcg
     1681 aagagggtgt ggagcgccag ccccatcagc gcggtggtcc cgactaccgg ctcgacccag
     1741 tgctcgtggt gggtctcctc gccgaagtgg aagtgcgcga ccaggacgtg ctcggcgaag
     1801 tggaccagca ggtagccgac gaggatcagc gttcccaccg acagcatcgc cgtctccccc
     1861 ttcagggcga ggcggtagct ctcggggacc atgcgcagga ggaccgccgc cagcatgaat
     1921 ccggcgccga tggcgacgaa gtaggactgg cggatcctgt cccagccgct cgctttcgcg
     1981 gcgaggaggc cgccgaagac gttggccccg cccgcgacga gcgcgcaggc gatcgccgtc
     2041 cacgccgggc cggcggtcat gacggtggtg gtgtgaaggg gctcacgcgg ctagcggccg
     2101 cggacgatcc cgggggaatg cgtggtcccg accgggacca ggagctgttc cttggcgtag
     2161 atgaaggagt cgcgcgagta gtcggaaagc tcgaaattgt gccccaggaa gatcgcgtcc
     2221 tcgggacagg cctcttcgca gtacccgcag aagatgcagc ggagcatgtc gatctcgtag
     2281 cgcttggcgt agcgctcccc cgccgagaag cgctccgtga cgctgttctc cccggcctcc
     2341 atgtagatgg cgtcggccgg gcagaccgcg gcgcagagac cgcagcagac gcaccgctcg
     2401 aggccgttcg agtagcggtg cagctcgtgg agccctcgga agcgcgggaa catcgtcatg
     2461 cgctggtcgg gatatttcag ggtaacgggc ttcttgaaga aatgcgagag cgtgatgctg
     2521 aggcccttca tgagttcgat cggagtgatg gccatcggcg cattataggg agcgggtgtc
     2581 tggcgggtca agcgggccgg gcgcgggcgg cgccctgccg gagctacgct tcggggcttc
     2641 ggccgtactc ttcggggcgg atgtcgagct tcttgatctt gtagaggagc gtggtgcgct
     2701 tcatcccgcc caggctgcgc gcggcctcgg actggttgcc gccgtgggac cggagggcat
     2761 gcaggatcac ctcgcgctcg gcgcttcccg cgcgcgtcgc ggcgcgccgc gctccgccgc
     2821 gcgagcccgg ccgggtggcg ggctggagct ggatggcggc cgcgtcgatc tcctcgccgt
     2881 cggtggccag ggcggccacg cgatccatca cattccgaag ctcgcgcacg ttgccgggcc
     2941 aggaatgcgt cgtgaggagc ttccaggcgt cgggcgtcag gcgcacgcgc tttccggctg
     3001 cggccgaggc gcgatccagc aggtactggc cgatcaggag cacgtcgtcg ggccgctcgc
     3061 ggagcggcgg cacttcgagg cggatcccgt tcagccggta gtagaggtcg tcgcggaaga
     3121 ggccgcggcg caccagcgcc gcgacgtcgc gggtcgaggc cgcgatcacg cgcacgtcca
     3181 cctgcgtgag gccggtgccg cccaggcgcc gcagctcgcc cgtctcgatc acgcgcaaca
     3241 gcttggcctg cgcgctggtc aagagctcgc tcacttcgtc caggaagatc acgccgcggt
     3301 tggcggcttc gaagaggccc gggcgcgagc cacgatggcc gctcacctcg cccagctcct
     3361 cgccgaacag gtcggcgtcc agctgacctt cgggaagggc cgcgcagtgg ccgaccacga
     3421 agatctctcc gcggcggtcg ctcagggcat gcgccgcccg cgcgagaagc tccttcccca
     3481 cgccgctctc gcccaggatc aggatcggaa gccggctcaa cgaggcgcgc tccagcacgc
     3541 gcaacagctc gcgcacgcgc gcgtcctgcg tcaccatgcc gaagcggaag cagtcgggat
     3601 cggccccttc gggcatcggg tcggccgcgt tccgccgcgc gggcgccggc gccgcggggc
     3661 ggagatccac gcccagcacc ttctcgatcc gctcgaccca gccgtccagc cgcatcgaca
     3721 tcgcgtggct ccgcgcttcc cacagatggt cgcgcgccgc gcgccgctcc tcggcgtcgc
     3781 cgccggcgag caaggtctcg cccagccaga ccagaaggcg cgtcagctcg tacaccgcct
     3841 ccagcttgcg gaggagggag acgccctcct gcgcctgacg cagcgccttc tcgcgatggc
     3901 ccaggaggtg gtgcgccatg gccatgacgc ggtgcgtcga cgcttcctcg aaccggtcg
//
LOCUS       DASSLZ010000141         4191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1822242, whole genome shotgun sequence.
ACCESSION   DASSLZ010000141 DASSLZ010000000
VERSION     DASSLZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822242"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1343
                     /gene="hpnE"
                     /locus_tag="VFM00_05315"
     CDS             30..1343
                     /gene="hpnE"
                     /locus_tag="VFM00_05315"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HET7497698.1"
                     /translation="MALPDVVVVGAGFAGVAAATALAERGASVHVLETRQRPGGRAYS
                     WTDPLTGELRDNGQHVLASFYDETWKLLRRIGTERELEADPAFRLRYWERGRGEYEIR
                     CPKLPAPFHWIAAIASCPRLSPAARLAAASLRPKAEALARDDADRRTVARWLREEVGA
                     PDLETILSPLALAALNESPDEGSAVLFARALDRLLGAPAASSGLALARRGLGDLLAGF
                     EAYVARQGGAVRYRSTALGVRVEQGRAAGVSLLGGERIEAGAVILAVPHERVTWLLRP
                     DLAEPYRAIAAVPWSPIVSTVHVYDRPVLPARFLGLIGTKTQWAFDRGAAGSGTGAGF
                     AVGTVRSAAFADTGCAPDAIAQETTADLAEAFGAARCARLLGARVYKERRATMRATPE
                     ADALRPPARTHVDGLVLAGDWTATGLPPTIESAALSGHRAAEALS"
     gene            1340..2491
                     /gene="mutY"
                     /locus_tag="VFM00_05320"
     CDS             1340..2491
                     /gene="mutY"
                     /locus_tag="VFM00_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET7497699.1"
                     /translation="MSPRDLAREGVVVSPRDLSALRSAILAWYRRNRRDLPWRRTRDP
                     YAIWVSEIMLQQTRVETVIPFYERFLKRFPTPRDLARAREADVLAVWSGLGYYRRARG
                     LHAAAAVVVREHGGRIPEELESLRSLPGIGAYTAAAIASVAHGRPAGAVDGNVIRVLA
                     RIAGFQGRRDDPALRRKVEAIATRLAEGDSPGDWTQALMELGALICVPRAPRCEACPA
                     ATRCLARRSGDPAAYPEPAKTGSSLRTRRIVMLLARRGAKILLLNGEAAGNAAEPKAW
                     SLPLIEMKTATSRSAAVRLSRTWTKGAPVKGPTRRFRHRTYAEDLHFEVWESLCPAIK
                     PRAAEPAKKAAATRIAGVRWVDPKRLDELPLRSPTLKAVKGLRRSPKLP"
     gene            2576..>4191
                     /locus_tag="VFM00_05325"
     CDS             2576..>4191
                     /locus_tag="VFM00_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7497700.1"
                     /translation="MRRLPRPFTPAAILLSLALFASPSNATPRSVSPALAHIEPGTDL
                     SDRFLTKGRNGETMIELIVEGDVAPGLLRARGIEVNTVAGRMMTARCPVGLLQALLAM
                     PYIDRVSVAERCKSNLDRSAPDTHVNALRTITPPVITGQTGAGVLVGDVDTGVDLGHL
                     DFKHQDGTTRLLSVWDQTATTGTPPAPFTYGAEFTAAQINAGTATETDTDGHGTHVLG
                     IAGGNGRATGNGQPAFTFVGMAPEADLVMVKTTFATSAIIDGVNYVFQRAAALGKQAV
                     VNLSLGTEDGPHDGTYPFDTFVNALTGPGKIVVASSGNTGEDDMHGRLDLNGTTPQSM
                     TVTVPTYTKNAGTQNDYLLFSGWYAGADQVSLTIVTPSGTTIGPIATGLSSTANNTGD
                     GYINAYNGTTTPANGDHEIYVEIFDAFANKAPKNGTWEFRFTPVSLTTTGIADMYLYG
                     DHLGNGAALARFAQGLSFGGVVGSPGSADSVITVGAHVTKECWTAVDGSRYCWNPTPT
                     VNSIASFSSQGPRRDGALKPDLTAPGFGVVS"
BASE COUNT          622 a         1528 c         1446 g          595 t
ORIGIN      
        1 cgctcgccgt gcgcagcccg cgctgacgcg tggcgctccc tgacgtcgtg gtcgtgggcg
       61 ccggctttgc cggcgtggcg gccgcgaccg ccctggccga acgcggagcc tcggtgcacg
      121 ttctggagac ccggcagcgc ccgggtggac gcgcctactc ctggaccgat cccctaacgg
      181 gcgagctccg cgacaacgga cagcacgttc tcgcctcctt ctacgacgag acctggaagc
      241 tgctccgccg catcggtacc gagcgcgagc tggaggccga ccccgcgttc cggctccggt
      301 actgggagcg cggccgcggc gagtacgaga tccgctgtcc gaagctcccc gcgccgtttc
      361 actggatcgc cgcgatcgcg tcctgcccgc gcctgtcgcc cgccgcgcgc ctggccgcgg
      421 cgtcgctgcg tcccaaggcg gaagccctcg cccgcgacga cgccgaccga cgcacggtcg
      481 cgcggtggct ccgggaggaa gtcggagcgc ccgatctcga gacgatcctg agtcctttgg
      541 ccctcgcggc gctgaacgaa tcgccggacg agggatcggc ggtgctcttc gcccgtgcgc
      601 tcgaccggct cctgggcgcg cccgcggcgt cgagcggcct cgcgctcgcg cggcgcggcc
      661 tgggcgatct gctcgcggga ttcgaggcgt acgtggcgcg tcagggcggc gccgtgcgct
      721 accgctccac cgcgctcggg gttcgcgtcg agcagggacg cgcggccggc gtctcgctct
      781 tgggcgggga gcggatcgag gcgggagcgg tgatcctggc cgtgccgcac gagcgggtga
      841 cctggctgct tcgccctgac ctggccgagc cctaccgcgc catcgccgcg gttccctggt
      901 ctcccatcgt ctccaccgtg cacgtgtacg atcggccggt gcttcccgcg cgattcctcg
      961 gtctcatcgg aacgaagacc cagtgggcgt tcgaccgcgg cgccgcgggc tccgggacgg
     1021 gcgctggctt cgcggtgggg acggtgcgga gcgccgcgtt cgccgacacg gggtgcgcgc
     1081 ccgatgcgat cgcccaggaa accaccgccg acctggcgga agccttcggc gcggcgcgct
     1141 gcgcgcggct cctcggcgca cgcgtctaca aggagcggcg cgccacgatg cgggccacgc
     1201 ccgaggcgga tgcgctgcgc ccgccggccc ggacgcacgt ggacgggctc gtcctggcgg
     1261 gcgattggac ggccacgggg ctgcccccca cgatcgagag cgcggcgctg agcggccatc
     1321 gagcggccga ggcgttgtcg tgagcccccg cgacctggcc agggagggcg ttgttgtcag
     1381 tccccgcgac ttgtccgcgc tccgttcggc catcctggcc tggtaccggc ggaaccgccg
     1441 cgaccttccg tggcggagaa cgcgcgatcc ctacgcgatc tgggtgagcg agatcatgct
     1501 ccagcagacg cgcgtcgaga cggtgatccc cttttacgag cggttcctga aacgctttcc
     1561 caccccgcgg gacctggcgc gggcgcgcga ggcggacgtg ctcgccgtgt ggtcggggct
     1621 gggctactac cgccgggccc gcggacttca cgccgcggcg gcggtcgtcg tgcgcgagca
     1681 cggcgggagg attcccgagg agctggaatc gcttcgctcg ctgccgggga tcggcgccta
     1741 caccgccgcc gcgatcgcga gcgtcgccca cggcaggccc gccggggccg tcgacgggaa
     1801 cgtgatccgc gtgctcgccc ggatcgccgg cttccagggc cggcgcgacg atcccgctct
     1861 gcgtcgaaag gtcgaagcga tcgcgacgcg attggccgag ggcgatagcc ccggggattg
     1921 gacccaggcg ttgatggagc tgggtgcctt gatctgcgtc ccgcgcgcgc cgcgatgcga
     1981 ggcctgtccc gccgcgaccc gctgcctggc gcgccggagc ggcgatccgg ccgcctaccc
     2041 agaacccgcg aaaaccggtt cgagcctgcg aacgcggcgc atcgtgatgc tcctcgcgcg
     2101 gcgcggcgcg aagatcctgc tcctgaacgg cgaggccgcc ggaaacgccg cggagccgaa
     2161 agcctggagc cttcccctca tcgaaatgaa gacggcgacg tcacgatccg cggcggttcg
     2221 gctctcgcgc acgtggacga agggagcgcc cgtcaagggt cccacgcgcc gattccggca
     2281 tcgcacctac gccgaggatc tccacttcga ggtgtgggaa tcgctttgtc cggccatcaa
     2341 accgcgcgcc gcggagcccg ccaagaaggc agcggcgacc cggatcgccg gcgttcgctg
     2401 ggtcgacccg aagcgcctgg acgagctgcc gctccggagc cctactctca aggcagtcaa
     2461 aggcttacgc cgcagtccca agctcccctg aatcgatctt cgcgtggtat aatctaagac
     2521 tcgatctgca ctgtccgagc tgtacgagcc acacccgtgt cgaggtgatg agttcatgcg
     2581 ccgtctcccc cgccccttca cgcccgcagc gattctcctg tccctcgctc tcttcgcttc
     2641 cccttccaac gccacaccac gatccgtctc gcccgcgctc gcgcacatcg agcccgggac
     2701 cgacctgtcc gaccgtttcc tcacgaaggg acggaacggc gagacgatga tcgaattgat
     2761 cgtggaggga gacgtcgcgc cggggctcct ccgcgcgcgc gggatcgagg tgaacacggt
     2821 cgccggacgg atgatgaccg cgcgctgccc cgtcggcctc ctacaggcgc tgctcgcgat
     2881 gccgtacatc gaccgcgtct ccgtggcgga gcgctgcaag tcgaacctgg accggagcgc
     2941 gcccgacacg cacgtgaacg cgctccgcac gatcacgccg cccgtgatca ccggtcagac
     3001 cggggcgggc gtcctggtcg gcgacgtcga cacgggggtg gatctcggac atctggactt
     3061 caagcaccag gacggcacga cgcggctgct ctcggtgtgg gaccagaccg cgacgacggg
     3121 gaccccgccg gcgccattca cgtacggcgc ggagttcacg gcggcccaga tcaacgcggg
     3181 aacggcgacg gagaccgaca ccgacgggca cggcacgcac gtgctcggaa tcgccggggg
     3241 caacggccgt gcgaccggaa acggccagcc ggcgttcacc ttcgtcggca tggcgcccga
     3301 ggccgatctc gtcatggtca agacgacctt cgcgacctcg gccatcatcg acggcgtgaa
     3361 ctacgtcttc cagcgcgccg cggcgctcgg caagcaggcc gtggtgaacc tgagcctggg
     3421 caccgaggac gggccgcacg acggcacgta ccccttcgac acgttcgtga acgcgctgac
     3481 cggccctgga aagatcgtcg tcgcgtcgtc cgggaacacg ggcgaggacg acatgcacgg
     3541 gcggctcgac ctgaacggaa cgacgccgca gagcatgacg gtaacggtcc ccacgtacac
     3601 caagaacgcg ggcacccaga acgactacct gctcttctcg gggtggtacg cgggcgcaga
     3661 ccaggtctca ctcaccatcg tcaccccctc cggcaccacc atcggcccga tcgccacggg
     3721 gctttcgagc accgcgaaca acaccggcga cggctacatc aacgcctaca acggaacgac
     3781 gactccggcc aacggcgacc acgagatcta cgtcgagatc ttcgacgcgt tcgcgaacaa
     3841 ggctccgaag aacggcacgt gggagttccg cttcacgccc gtttccctca cgaccacggg
     3901 gatcgccgac atgtacctgt acggcgacca cctcggaaac ggcgccgcgc tggcgcgctt
     3961 cgcgcagggg ctctccttcg gcggggtcgt gggttctccc ggcagcgccg actcggtgat
     4021 cacggtcggc gcgcacgtga ccaaggagtg ctggacggcc gtggacggaa gccgctactg
     4081 ctggaatccg acgcccacgg tgaactccat cgcgtccttc tccagccagg gcccgcgcag
     4141 ggacggcgcg ctgaagcccg atctcacggc acccggattc ggcgtcgtct c
//
LOCUS       DASSLZ010000142        11107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1443490, whole genome shotgun sequence.
ACCESSION   DASSLZ010000142 DASSLZ010000000
VERSION     DASSLZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443490"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..896
                     /gene="pyrR"
                     /locus_tag="VFM00_05330"
     CDS             342..896
                     /gene="pyrR"
                     /locus_tag="VFM00_05330"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET7497701.1"
                     /translation="MAEQVREKTELIDGEGLRRAITRIAHEIIEKNGGAESVVLVGIR
                     RRGFPIAKRIAARIQEFEGADVPVGALDITLYRDDLQMIAHQPVVSGTEIPFDIDGKV
                     VVLVDDVLFTGRTVRAALDELIDFGRPRAIQLAVIVDRGHREIPIRADFVGKNVPTSK
                     REVIMVAVTEVDGHDGVALGELLT"
     gene            893..1870
                     /locus_tag="VFM00_05335"
     CDS             893..1870
                     /locus_tag="VFM00_05335"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET7497702.1"
                     /translation="MIGQRKDLLGLEDLSAEEIVSILDTARTFREVLERPIPKVPSLR
                     GLTAANLFFEPSTRTRLSFELAQKRLSADTVNFQTTGSSVSKGETLKDTARNIEAMGI
                     HLVVIRHQSSGAPHYLARNLDAAIINAGDGTHEHPTQGLLDIYTMRERKGRIDGLKVA
                     IIGDIMHSRVARSNIWGLTKLGASVTIAGPATMMPAEVERFGVTVASRVEEAIEGADV
                     VNILRIQLERQRAGLYPSLREYARVYGVTKERLQGARPDAIVMHPGPMNRGVEIAQEV
                     ADGPNSVILEQVTNGVAVRMAVLYLLSRPDEPLPAPEPAPRRAVEGAKR"
     gene            1867..3186
                     /locus_tag="VFM00_05340"
     CDS             1867..3186
                     /locus_tag="VFM00_05340"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET7497703.1"
                     /translation="MTRPICLRGGRVIDPASRVDRVEDLWLDQGKISGLGEEAPAALR
                     AHPDAEVIDVSGAVICPGFVDIHVHLREPGQEDKETVETGTRAAARGGFTTVACMPNT
                     SPPLDDRPRIEFVIRRGREAGHARVLPIAAVTRGQMGEALTEIEDLVDAGAVAFSDDG
                     KPVHNAEIMRRALELTRPLGVPVIQHAEDPDLKGSGVVHEGWIATRVGLKGIPDAAES
                     VMVARDALLAELTGGRVHVAHVSAARSVDIIRKAKARGIAMTAEVTPHHLILTDEAVS
                     GYDTNTKMNPPLRSTRDREALIEGVVDGTIDCLATDHAPHTEIEKDNDFDSAPFGILG
                     LETALGLYIKALVEPGHLTLPELLLRMTRRPLEVLGRQGGTLEPGEPADVTVFDPMGR
                     WTVVAKESASRSHNTPFDGWELPGRVLVTILDGQVTYRESASLASGR"
     gene            3222..5039
                     /locus_tag="VFM00_05345"
     CDS             3222..5039
                     /locus_tag="VFM00_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497704.1"
                     /translation="MSKIRPGTLRLRILVPILLLASSGCAYYNTFYLARRYYHDAEKE
                     QEHSVSDETSVAAKTKYDQTVRQCTKVITEYPKSKWVDDATYLLAASLYGKGDYTAAI
                     RRADDLELKFPKSPFVPEGRLVKGLSYYRRKDYDTADSIFQAMDAAYPKFPRRWELYY
                     YEGESRFAQRDYTGALGWYRRAVPHADGRRQKADALHRAADALFEADRMDSAQLVYAE
                     TLKEEERSKDRLNVILRRAEALRNLKRYPEALQFLNTWRSTAIAENREGELLLRVYEL
                     QALTGRTNDAMKGYQDLVERFPGTNVAFESQFQIGYLVETAVGDLDRAGREYDKLRTV
                     AGGNNQFAQQALRRSQSLATLKQFQQALKADTTGSRARTAFRLAEVLYFELGKTDSAM
                     TQYRSVEALYPQSVYAPKSAYARLWISAFDRQDTLGASQLTDSIAGRYRGTRYAESAL
                     YLWKQWSGKSDVRTALFDSLLAHPDTSGAARYAEETEPADSLTGIVPPTSASSDSTYQ
                     VSPQIQRDLEERARKFLADREAAKKAREAGKKPPAAPPPVPPKTAMPSAPDTTHAAPA
                     DTTRAKMPPPTAPPDTTRSTAPPDTTRSAAPPDSGGTAR"
     gene            5036..5887
                     /locus_tag="VFM00_05350"
     CDS             5036..5887
                     /locus_tag="VFM00_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET7497705.1"
                     /translation="MKLVPCKVIENREVSPGNFLLTLTVPRGFGTPNPGQFVHLRISE
                     EGDPLLRRPYSLEGFVSKGRVRAVRIYYSVVGRGSRILSLQPKGRVLDLIGPLGIGFA
                     PRPRRIPILVAGGRGIAPLLFLSRRLKEKKRPFVFLFGARSRKELYGVREVRGGRLHL
                     ATDDGSVGFHGSVFELLKREWSEGGHTPLSAEIFTCGPHGLLHEIADFARATGARCEA
                     SLEGPMACAVGACRGCPVPLLPGAENGAAHVGNGAAGGVPGGRYPAMCTEGPVMDATI
                     VDWERLP"
     gene            5884..6870
                     /locus_tag="VFM00_05355"
     CDS             5884..6870
                     /locus_tag="VFM00_05355"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET7497706.1"
                     /translation="MTPAKHPSLAVRIGSLALRNPILVASGIIGYGAEYEALVDLNAI
                     GGIVTKTVTRHPRAGNPPPRVVEVPSGMLNSIGIENPGLEGFLEIKVPILRRLPCAVI
                     VSVEGEDTREFCELVEGVSGSGVAHAIEVNISCPNVGPHGLKYSTDAGMAREVMSAIR
                     PLTKLPLIAKLTPNVTRIGEIAEACASCGADAVSLVNTFVGMAVDSRTHRPILGTVLG
                     GLSGPAIKPLALAKTWEVVQAVDIPVIGMGGIAKPADALEFLLTGAVAVQVGTALFAN
                     PALAEECVHGIEHHLRRIGASSVEAIIGALEVPEGRGTIRAGRQARIGTGGA"
     gene            6867..7628
                     /gene="pyrF"
                     /locus_tag="VFM00_05360"
     CDS             6867..7628
                     /gene="pyrF"
                     /locus_tag="VFM00_05360"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET7497707.1"
                     /translation="MTEPWAPASSPVHLRERLIVALDRPDLNAALEQVDRVGDAVLWY
                     KVGLQLFCAAGRGAVLALADRGKRVFLDLKLHDIPATVERAIRALEGLPVSLLTVHAS
                     GGPDMLRAAAEAARYLAGHPRVLGVTMLTSLDGTEIPPLFNPKTEIEEKVLGLAALCA
                     TSGVDGVVASPREIQPLRLEHPAPFLIVTPGIRGPNDPAHDQKRTLSLAEAFARGADY
                     VVVGRPILESADPRGTVAAYEASLAAAFSKERADA"
     gene            7625..8575
                     /locus_tag="VFM00_05365"
     CDS             7625..8575
                     /locus_tag="VFM00_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET7497708.1"
                     /translation="MKVLITGITGFAGSHLADYILQRGDAEVIGILRWRSRTENIEHF
                     RDKVRLVECDLRDASSVRDVLDETRPDWIFHLAAQSFVPTSWTAPTESLVTNILGQLN
                     IFEAVKKLHLMPRIQLACSSEEYGLVHEDELPIKETNPLRPLSPYAVSKVGQDMLGYQ
                     YWMSFKLPVIRTRGFNHEGPRRGPVFVCSDFAKQIADIEKGRRPPVVRVGNLDARRDF
                     TDVRDIVRAYWLALEKGEPGEVYNLCSGRAWTIREMLDMLLAMSKTKIKVEQDEARMR
                     PSDVPVLLGDATRFRNRTGWEPSIPFEQTMRDLLAYWRER"
     gene            8580..9554
                     /locus_tag="VFM00_05370"
     CDS             8580..9554
                     /locus_tag="VFM00_05370"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET7497709.1"
                     /translation="MRTFVTGVAGFVGRHLMRALTVEGPADVHGADHMAAEAIPEPEE
                     LRASLQSYRALDVTDADAVRAAIAELRPNRIIHLAAQASGAVSLEQPAETYRVNAIGA
                     LNVLEACRAAAGDATVLIVGSADVYGSGAPGTKLTEEAPLAPRNPYALSKAAQDMLGE
                     LYASSYGLRVLRTRTFSHTGPGQSPRFAVAGFAHQLARVLEGDGPAEVRAGNLDVVRE
                     YGDARDVVRAYLALLESGEPGQAYNVCTGQGYRLGDLLDKLIQVSGVRASVVQDPSRM
                     RSRDADHLVGDPEKVIRRTGWAPAIPIERTLLDLYRDARERVRHEAGR"
     gene            9565..10869
                     /locus_tag="VFM00_05375"
     CDS             9565..10869
                     /locus_tag="VFM00_05375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET7497710.1"
                     /translation="MRIVVVGSGYVGLVSGAGFADFGNEVLCVDVDERKIDVLSRGEI
                     PFYEPGLKDLVLRNTRERRLRFGTSLADAVRWGDVIFLCVGTPMRADGGADLTQVLAA
                     ARTIARSMPGYRLIVQKSTVPVGTADKLRAWMKRYAPRGAKFDVASNPEFLREGSAVE
                     NFMRPDRIVIGADAPRPAEILRELYRPLHLIETPAVVVNVRTAELIKYASNAFLAIKI
                     SFINEVANLCEAVDADVHDVARAMGLDRRIGPKFLHPGPGYGGSCLPKDTHAMRAFSR
                     KAGVRLRVVEAAIETNDAQHQVATKKVLAALGGRGKRVAAVLGLSYKPDTDDLREAPS
                     LYIIRALLRKGIAIRAYDPAVRADAEGLPGGVHFAQNPYDAVKGADVMVLVTEWNEFR
                     RLDLARVKRAMRRKSIVDLRNVYDPATVKRLGFAYISVGRPA"
     gene            10866..>11107
                     /locus_tag="VFM00_05380"
     CDS             10866..>11107
                     /locus_tag="VFM00_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET7497711.1"
                     /translation="MKLIDGVHVKPLKVIADERGYLMEMMRKDDPFFQRFGQTYVSVA
                     YPGVVKGWHYHKIQTDHFVIVKGMMKVVLYDDREGSP"
BASE COUNT         1778 a         3893 c         3864 g         1572 t
ORIGIN      
        1 aaccgagcac ctgtgccgag agtcagtgtc cgagcgccgc cgccgcggtc atggccgagc
       61 caaccgtgac catggtgacc gcgaccaagg cgtcgcggcg caggaagaac cccgtgacga
      121 cgaatcccgt gccgatcagc agagcgcggg gggccggggt tgtccggcgc ccccgttcgt
      181 ggtatcgtca ccgacgcgtc gcaagcatcg cgcggcgccc gcgacccttg aatctagccc
      241 gagagactag aaagggaccg agccgaagca ctggccagcc gcccctttcc ggtccgggag
      301 aggggttttt tcatgcccct ccgcgtcccc caggagatcc gatggccgag caggtccgcg
      361 aaaagaccga gctgatcgac ggcgaggggc tgcgccgggc catcacccgc atcgcgcacg
      421 agatcatcga gaagaacggc ggcgcggaga gcgtggtcct ggtcgggatc cggcgccgcg
      481 gctttccgat cgcgaagcgg atcgcggcca ggatccagga gttcgagggg gcggacgtgc
      541 ccgtgggggc gctcgacatc acgctctacc gggacgacct ccagatgatc gcccaccagc
      601 ccgtcgtcag cggcaccgag atccccttcg acatcgacgg gaaggtcgtg gtgctggtgg
      661 acgacgtgct cttcacggga cgcaccgtgc gcgcggcgct ggatgagctg atcgacttcg
      721 gccgcccgcg cgccatccag ctcgcggtca tcgtggaccg cggccaccgc gagatcccga
      781 tccgcgccga cttcgtcggc aagaacgtcc ccacgtccaa gcgcgaggtc atcatggtcg
      841 ccgtcaccga agtcgacggc cacgacggcg tcgcgctcgg ggagcttctg acatgatcgg
      901 ccaacggaaa gacctgctcg gcctggaaga cctgagcgcc gaggagatcg tctcgatcct
      961 ggacaccgcg cggacgttcc gcgaggtgct cgagcgtccc atccccaagg tgccctcgct
     1021 gcgcgggctg accgccgcga acctcttctt cgagccgagc acccgcacgc gcctctcctt
     1081 cgagctggcg cagaagcgcc tctcggccga caccgtgaac ttccagacga ccggctccag
     1141 cgtctcgaag ggggagaccc tcaaggatac ggcgcggaac atcgaggcga tggggatcca
     1201 cctggtcgtg atccgccacc agtcctcggg ggcgccgcac tacctggcgc ggaacctgga
     1261 cgcggcgatc atcaacgcgg gcgacggcac gcacgagcac ccgacgcagg ggctcctgga
     1321 catctacacc atgcgcgagc ggaaggggcg gatcgacggc ctcaaggtcg ccatcatcgg
     1381 cgacatcatg cacagccgcg tggcgcggtc gaacatctgg gggctgacca agctgggcgc
     1441 ctcggtgacg atcgcgggtc ccgcgacgat gatgcccgcc gaggtggagc gcttcggcgt
     1501 caccgtggcc tcgcgcgtcg aggaagcgat cgagggggcc gacgtcgtga acatcctccg
     1561 catccagctg gagcgccagc gcgccgggct ctatccatcg ctccgcgagt acgcgcgggt
     1621 gtacggcgtg accaaggagc gcctccaggg cgcgcggccc gacgcgatcg tgatgcaccc
     1681 cgggcccatg aaccgcggcg tcgagatcgc gcaggaggtg gccgacggac cgaactccgt
     1741 gatcctggag caggtgacga acggcgtggc cgtgcggatg gcggtgctct atctcctctc
     1801 gcggcccgac gagccgctcc ccgcgccgga gcccgcgccg cgccgcgcgg tcgagggggc
     1861 gaagcgatga cccggccgat ctgccttcgg ggcggccgcg tgatcgatcc cgcgagcagg
     1921 gtcgaccgcg tggaagacct ctggctggac cagggaaaga tctccggcct gggcgaggaa
     1981 gcgcccgcgg cgctccgcgc ccaccccgac gccgaggtga tcgacgtctc gggcgcggtg
     2041 atctgcccgg ggttcgtgga catccacgtc catctgcgcg agccgggcca ggaggacaag
     2101 gaaaccgtgg aaaccggcac gcgcgcggcg gctcgcggcg gcttcaccac ggtcgcctgc
     2161 atgccgaaca ccagcccgcc gctcgacgat cggccgcgca tcgagttcgt gatccgccgg
     2221 gggcgcgagg cgggccacgc gcgcgtcctt ccgatcgcgg ccgtgacgcg cggccagatg
     2281 ggcgaggcgc tcaccgagat cgaggatctg gtcgacgcgg gcgcggtggc tttctccgac
     2341 gacggaaagc cggtccacaa cgccgagatc atgcgccgcg cgctggagct gacgcgcccg
     2401 ctgggcgtgc cggtgatcca gcacgctgag gacccggact tgaagggctc gggcgtcgtc
     2461 cacgagggct ggatcgcgac acgggtcggg ctcaagggga tcccggacgc cgccgagtcg
     2521 gtcatggtcg cgcgcgacgc gctcctcgcc gagctgacgg gcggccgggt ccacgtggcg
     2581 cacgtgagcg cggcccggtc ggtcgacatc atccgcaagg ccaaagcgcg cggcatcgcc
     2641 atgacggccg aggtgacgcc gcaccatctg atcctcaccg acgaagcggt ctcgggctac
     2701 gacaccaata ccaagatgaa tcccccgctc cgctccacgc gcgaccgcga ggcgctgatc
     2761 gagggggtgg tggacggcac gatcgactgc ctggccaccg accacgcgcc ccacaccgag
     2821 atcgagaaag acaacgactt cgattcggct ccattcggga tcctggggct cgagacggcc
     2881 ctgggcctct acatcaaggc cttggtcgag ccggggcacc tcacgctgcc cgagctcctc
     2941 ctccggatga cccgccgccc cctggaagtg ctgggacggc aagggggaac gctggaaccc
     3001 ggagaaccgg cggacgttac ggtgttcgac ccgatgggac gttggacggt cgtcgccaaa
     3061 gagtcggcct cccgctccca caacaccccc ttcgatggtt gggagctgcc cgggcgcgtc
     3121 ctggtcacca ttctggacgg gcaggtcacc taccgcgaat ccgcatcctt ggcgagcggc
     3181 cgatagcccg tcggcctcgc gcgtggtagc atccatcgct catgtcgaaa atccgaccgg
     3241 ggacgcttcg tctccgaatc ctcgtgccca tcctgctcct ggcgtcatcc ggatgcgctt
     3301 actacaacac gttttacctg gcgcggcggt actaccacga cgccgagaag gagcaggagc
     3361 atagcgtctc cgacgagacc tccgtggccg ccaagacgaa gtacgaccag acggtccgcc
     3421 agtgcaccaa ggtgatcacc gaatacccca agagcaagtg ggtggatgac gccacctacc
     3481 tcctcgccgc ctccctctac ggcaaggggg actacaccgc ggccatccgg cgcgccgacg
     3541 acctggagct caagttcccc aagagcccgt tcgtgcccga ggggcggctg gtgaagggac
     3601 tctcctacta ccggcggaag gactacgaca ccgccgattc gatcttccag gccatggacg
     3661 ccgcctatcc gaaattcccc cgccgctggg agctctacta ctacgagggg gagagccggt
     3721 tcgcgcagag ggattacacg ggggccttgg ggtggtaccg tcgcgccgtt ccgcacgcgg
     3781 acggacggcg gcagaaggcc gacgcgctcc accgcgcggc ggacgcgctg ttcgaggccg
     3841 atcgcatgga cagcgcgcag ctcgtctacg cggagaccct gaaggaagag gagcgctcga
     3901 aggatcgcct gaacgtgatc ctccggcggg cggaggcgct ccggaatctg aagcgctatc
     3961 cggaggcgct ccagttcctg aacacctggc gctcgacggc gatcgccgag aaccgcgagg
     4021 gagagctcct gctcagggtg tacgagctgc aggcgttgac gggcaggacc aatgacgcga
     4081 tgaaagggta ccaggacctg gtggagcgct ttcccggaac gaacgtggcg ttcgaatcgc
     4141 aattccagat cggctacctg gtcgagaccg ccgtcggcga cctcgaccgg gcggggcgcg
     4201 agtacgacaa gctgcgcacg gtggcgggcg gcaacaacca gtttgcccag caggcgctgc
     4261 gccgctcgca gagcctggcg acgctgaagc agttccagca ggcgctcaag gccgacacga
     4321 ccgggtcccg cgcgcgtacc gccttccgcc tggccgaggt cctctacttc gagctgggga
     4381 agaccgactc ggcgatgacg cagtaccggt ccgtcgaggc gctctacccc cagagcgtgt
     4441 acgcgccgaa gtcggcctac gcgcggctct ggatctcggc gttcgaccgt caggacaccc
     4501 tcggcgcgtc gcagctcacc gactcgatcg cggggcgcta ccgcggcacg cgctacgccg
     4561 agtcggcgct ctatctctgg aagcagtgga gcgggaagag cgacgtccgc accgcgctct
     4621 tcgactcgct gctcgcgcac cccgacacct cgggcgccgc gcggtacgcc gaggagaccg
     4681 agcccgccga ttcgctgacc ggcatcgtgc cgccgacgag cgcgtcttcg gacagcacct
     4741 atcaggtctc gccgcagatc cagcgggatt tggaggagcg ggcgcggaag ttcctcgccg
     4801 accgcgaggc cgcgaagaag gcacgcgagg ccggtaagaa gcccccggcc gcgccgccgc
     4861 cggttccgcc caagaccgcg atgccctctg cgccggacac cacgcacgca gcgcccgccg
     4921 acacgacgcg cgcgaagatg ccgccgccca cggcaccgcc cgacacgacg cgctcgacgg
     4981 cgccgcccga cacgacgcgc agcgcggctc cgcccgacag cggggggacc gcgcgatgaa
     5041 gctcgttccc tgcaaggtga tcgagaaccg ggaggtctcg cccggcaatt tcctcctcac
     5101 gctcacggtg ccgcgcggat tcgggacgcc caacccgggg cagttcgtcc atctccggat
     5161 ctccgaagag ggcgatccgc tcctgcgccg gccgtacagc ctcgaaggct tcgtgagcaa
     5221 agggcgcgtg cgggccgtgc gcatctacta ttcggtggtg ggccgcgggt cgcgcatcct
     5281 ctccctgcag ccgaaggggc gggtgctgga cctgatcggg ccgctcggga tcggcttcgc
     5341 gccgcgcccc cgccgcatcc cgatcctagt cgccggaggc cgtggcatcg ccccgctcct
     5401 cttcctgagc cgccggctca aggagaagaa gcgccccttc gtcttcctct tcggcgcacg
     5461 ctcgcggaaa gagctgtacg gcgtgcgcga ggtgcgcggc ggtcggctgc acctggcgac
     5521 cgacgacgga tcggtcggat tccacggcag cgtcttcgag ctcctgaagc gggagtggag
     5581 cgagggagga cacacaccgc tctcggcgga gatcttcacc tgcggcccgc acgggctgct
     5641 ccacgagatc gccgacttcg cccgggccac gggcgcgcgc tgcgaggcct cgctcgaggg
     5701 gccgatggcc tgcgcggtcg gcgcgtgccg cggctgcccg gtgccgctgc ttcccggcgc
     5761 cgaaaacggc gcggcgcacg tggggaacgg cgcggccggc ggcgtgcccg gaggacgcta
     5821 ccccgccatg tgcaccgagg gacccgtgat ggacgccacc atcgtcgact gggagcgcct
     5881 gccgtgacgc ccgccaagca cccttcgctc gccgtgcgga tcggatcgct cgcgctccgg
     5941 aatccgattc tggtcgcgtc ggggatcatc ggctacggcg ccgagtacga ggcgctcgtg
     6001 gacctgaatg cgatcggcgg catcgtcacg aagaccgtga cgcgccatcc gcgcgccggc
     6061 aacccgccgc cgcgcgtcgt ggaggtcccg tcgggaatgc tgaactcgat cgggatcgag
     6121 aaccccgggc tcgagggctt cctcgagatc aaggtcccga tcctgcgccg cctgccctgc
     6181 gcggtgatcg tctcggtcga gggagaggac acgcgcgagt tctgcgagct ggtcgaaggg
     6241 gtgagtggaa gcggcgtcgc ccacgcgatc gaggtgaaca tctcctgccc caacgtgggg
     6301 ccgcacgggc tcaagtattc caccgacgcg ggcatggctc gcgaggtgat gagcgccatc
     6361 cggccgctca ccaagcttcc gctgatcgcc aagctgacgc cgaacgtgac ccgcatcggc
     6421 gagatcgccg aggcgtgcgc ctcgtgcggc gccgacgcgg tctcgctcgt gaacacgttc
     6481 gtcgggatgg cggtggactc gcgcacgcac cgtccgatcc tgggcacggt gctggggggg
     6541 ctctccggcc ccgcgatcaa gccgctcgcc ctggcgaaga cctgggaagt ggtccaggcc
     6601 gtggatatcc cggtgatcgg catgggtggg atcgcgaagc ccgccgacgc cctcgaattc
     6661 ctcctgaccg gcgccgtcgc ggtgcaggtc gggaccgcgc tcttcgccaa ccccgcgctc
     6721 gccgaagagt gcgtgcacgg catcgagcac cacctgcgcc ggatcggcgc ctcgtcggtc
     6781 gaggcgatca tcggcgccct ggaagtcccc gagggccggg gaacgatccg cgccggacgc
     6841 caggcgcgca tcggaacggg aggggcatga cggaaccctg ggcgccggcc tcttcgccgg
     6901 tccatctccg cgagcggctg atcgtcgcgc tcgaccgccc cgatctgaac gcggcgctcg
     6961 agcaggtcga ccgggtcggc gacgcggtcc tctggtacaa ggtggggctg cagctcttct
     7021 gcgcggccgg ccgcggcgcg gtgctcgcgc tggccgaccg cgggaagcgc gtcttcctcg
     7081 atctcaagct tcatgacatc ccggccaccg tcgagcgcgc gatccgcgcg ctggaggggc
     7141 tgccggtctc gctcctcacg gtgcacgcga gcggcggccc cgacatgctc cgcgccgcgg
     7201 cggaggcggc ccgttacctg gccggccatc cgcgggtgct cggcgtgacc atgctcacca
     7261 gcctggacgg caccgagatc ccgcccctct tcaatccgaa gaccgagatc gaggagaagg
     7321 tgctcggact ggccgcgctc tgcgccacat cgggcgtgga cggcgtggtc gcctcgccgc
     7381 gcgagatcca gccgctccga ctggagcatc cggcgccgtt tctcatcgtg acccctggaa
     7441 tccgcgggcc gaacgatccg gcccatgatc agaagcgcac gctctctctg gcggaagcgt
     7501 tcgcgcgggg ggccgactac gtcgtcgtgg gccgccccat tctcgaatcc gccgacccgc
     7561 gcggaacggt agcggcctac gaggcctcac tcgccgccgc gttctccaag gagagggccg
     7621 acgcatgaag gtgttgatca cgggcattac ggggttcgcc ggcagtcatc tcgccgacta
     7681 catcctccag cgcggcgacg cggaggtgat cgggatcctc cgttggagga gccgcaccga
     7741 gaacatcgag cacttccgcg acaaggtgcg gctggtggag tgcgacctcc gggacgcctc
     7801 ttccgtgcgc gacgtgctcg acgagacgcg ccccgactgg atcttccatc tcgcggccca
     7861 gagcttcgtc cccacgtcgt ggacggcgcc gacggagtcg ctcgtcacga acatcctggg
     7921 ccagctcaac atcttcgagg cggtgaagaa gctgcacctc atgccgcgca tccagctggc
     7981 ctgctccagc gaggagtacg gtctggtgca cgaggacgag ctgccgatca aggagaccaa
     8041 cccgctccgc ccgctctccc cctacgcggt gagcaaggtg gggcaggaca tgctgggcta
     8101 ccagtactgg atgagcttca agctcccggt catccgcacg cgcggcttca atcacgaggg
     8161 cccgcggcgc ggaccggtct tcgtctgctc cgatttcgcg aagcagatcg ccgacatcga
     8221 gaagggtcgg cgccctccgg tcgtgcgcgt ggggaacctg gacgcgcggc gcgacttcac
     8281 ggacgtccgc gacatcgtgc gcgcctactg gctcgcgctg gagaaggggg agccgggcga
     8341 ggtctacaac ctctgctcgg ggcgcgcctg gaccatccgc gagatgctcg acatgctgct
     8401 ggcgatgtcg aagaccaaga tcaaagtcga gcaggacgag gcgcgcatgc gcccgtccga
     8461 cgtgccggtg ctgctcggcg acgcgacgcg gttccggaac cgcaccggtt gggagccctc
     8521 gatccccttc gagcagacga tgcgcgacct gctcgcctac tggagagagc gctagaccct
     8581 tgcgcacgtt cgtcaccggc gtcgccggat tcgtcggacg ccacctgatg cgggcgctca
     8641 cggtggaggg cccggccgac gtgcacggcg ccgaccacat ggcggcggag gccatccccg
     8701 agccggaaga gctgcgtgcc tcgctccaga gctaccgcgc gctcgacgtg accgacgcgg
     8761 acgcggtgcg cgcggcgatc gcggagctcc gccccaaccg gatcatccac ctcgcggccc
     8821 aggcctccgg agcggtgtcg ctggaacagc cggccgagac ctatcgcgtg aacgcgatcg
     8881 gcgcgctcaa cgtactcgaa gcgtgccgcg ccgccgcggg cgacgccacg gttctcatcg
     8941 tcggctccgc cgacgtgtac ggctcgggcg cgcccgggac caagctgacc gaggaggcgc
     9001 cgctcgcccc gcggaatccc tacgcgctga gcaaggccgc gcaggacatg ctgggcgagc
     9061 tctacgcctc gtcctacggg ctccgggtgc tgcggacccg gaccttctcg cacaccggcc
     9121 ccgggcagag cccgcgcttc gcggtggcgg gcttcgcgca ccagctcgcg cgcgtgctcg
     9181 agggagacgg tcccgccgaa gtgcgcgcgg gcaacctgga cgtggtgcgc gagtacggcg
     9241 acgcgcggga cgtggtgcgc gcctacctgg cgctcttgga gagcggcgag ccggggcagg
     9301 cctacaacgt ctgcaccggc cagggttacc gcctgggcga tctcctcgac aagctgatcc
     9361 aggtttccgg ggtccgcgcc tcggtggtgc aggacccctc gcggatgcgc tcccgcgacg
     9421 ccgaccatct ggtcggtgac cccgaaaaag tgatccggcg caccggatgg gcgccggcca
     9481 tccccatcga acggacgctc ctcgacctct accgcgacgc gcgcgagcgc gtccggcacg
     9541 aggcagggcg ataagggagg aacggtgcgg atcgtcgtgg tcggttccgg atacgtcggc
     9601 ctggtgagcg gcgccggatt cgcggacttc ggcaacgagg tcctgtgcgt ggacgtggac
     9661 gagcggaaga tcgacgtgct ctcgcgcggc gagatcccct tctacgagcc ggggctgaaa
     9721 gacctggtgc tccggaacac gcgggagcgc cgcctgcgct tcggcacgtc gctggccgac
     9781 gccgtgcgct ggggcgacgt gatctttctc tgcgtgggca cgccgatgcg cgccgacggc
     9841 ggcgccgatc tcacgcaggt gctcgcggcg gcgcggacca tcgcgcgctc gatgccgggc
     9901 taccggctga tcgtccagaa gagcacggtg ccggtcggca cggccgacaa gctccgcgcc
     9961 tggatgaagc gctacgcccc gcgcggcgcc aagttcgacg tcgcctccaa cccggagttc
    10021 ctgcgcgagg gatcggcggt cgagaacttc atgcgacccg accggatcgt gatcggggcc
    10081 gacgcgcccc gccccgcgga gatcctgcgc gagctctatc gcccgctcca cctgatcgag
    10141 acgccggcgg tggtcgtgaa cgtgcgcacc gccgagctga tcaagtacgc ctcgaacgcg
    10201 ttcctcgcga tcaagatctc cttcatcaac gaggtcgcga acctgtgcga ggcggtggat
    10261 gccgacgtcc acgacgtggc gcgggccatg gggctggacc ggcgcatcgg gcccaaattc
    10321 ctccacccgg gtcccggcta cggcggctcc tgcctgccca aggacacgca cgccatgcgc
    10381 gccttctcgc gcaaggcggg cgtgcggctt cgcgtggtgg aggccgcgat cgagaccaac
    10441 gacgcccagc accaggtggc gacgaagaag gtgctcgcgg cgctgggcgg gagggggaag
    10501 cgggtcgcgg ccgtgctcgg cctctcctac aagccggaca ccgacgacct gcgcgaggcg
    10561 ccttcgctct acatcatccg tgccctgctc cggaagggga tcgccatccg ggcgtacgat
    10621 cccgccgtgc gcgccgacgc cgaagggttg ccgggcgggg tccacttcgc ccagaacccg
    10681 tatgatgcgg tgaagggtgc cgacgtgatg gtgctggtga cggagtggaa cgagttccgc
    10741 cgcctcgatc tcgcgcgcgt caagcgcgcc atgcggcgca agagcatcgt cgaccttcga
    10801 aacgtctacg acccagccac ggtgaaacgg ctgggcttcg cctacatctc ggtggggagg
    10861 cccgcatgaa gctgatcgac ggtgtgcacg tgaagccgct caaggtgatc gcggacgagc
    10921 gcggctacct gatggagatg atgcgtaagg acgatccctt cttccagcgc ttcggccaga
    10981 cctacgtctc ggtcgcctat cccggcgtgg tgaaggggtg gcactaccac aagatccaga
    11041 ccgatcactt cgtgatcgtg aaggggatga tgaaggtggt gctctacgac gaccgcgagg
    11101 gctcgcc
//
LOCUS       DASSLZ010000143         4683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1823246, whole genome shotgun sequence.
ACCESSION   DASSLZ010000143 DASSLZ010000000
VERSION     DASSLZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823246"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..2864
                     /locus_tag="VFM00_05385"
     CDS             18..2864
                     /locus_tag="VFM00_05385"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET7497712.1"
                     /translation="MPERVLSPAPRKAVYFFGDGKAEGDARMRDLLGGKGAGLAEMMN
                     AGVPVPPGYTITTEACRLFFESGGRLPESIAREEKAMLARLERLLGRRLGDPENPLLL
                     SVRSGSPVSMPGMMDTILNLGLNDETVEGLARKTRNARFAYDSYRRFIQMFADVVLGV
                     PRGLLEGELTAAKRRARVKSDVELGEADLRPLCARMLRIVRDETGKAFPQDAKEQLAA
                     ARDAVFRSWHTDRAVHYRALNGIPETLGTAVNVQAMVFGNLGATSATGVGFTRDPSTG
                     EPRFYGEYLMNAQGEDVVAGIRTPHPIEMMEKELPAAYAQLRQITERLERHYRDVQDF
                     EFTVEEGRLFMLQTRRGARTGIAALRIAVDMVDEGLLGKREALERVEPDHLDQVLHPV
                     LDPAARKKHALLAKGLPASPGASAGAIVFTSEEAVARGKKERVLLVRAETSPDDISGM
                     NAAQGILTATGGMTSHAAVVARGMGKCCVAGCTALVIDEDKRTLRIGSRTFPEGAFLS
                     LDGSTGEVLEGDVPTSDSEVVRVLTGRMTPAQAPLYQRFERLMAWCDEVRRLRVRANA
                     DIPRDAKVARALGAEGIGLCRTEHMFFAEDRIPHVVTMILNAKEARQARLRIARIESD
                     LAEAGKSDRPRLAKELARAKKEGKGAIQAFDGALAKLLPLQRADFKGLFLAMEGTPVT
                     IRTLDPPLHEFLPKREQLLAEMASLRRAKKDDPRRARLERTLERVEELHEFNPMLGFR
                     GCRLGILHPEITRMQARAIFEAAAQVAKEGLPVRPEIMIPLVGDREELRRQRAEVESV
                     AKEVMAKLGTTIPYTIGTMIEIPRAAMLADEIAAHADFFSFGTNDLTQMSFGFSRDDC
                     GRFLPEYVEAGILPIDPFVSLDQRGVGVMIQRACFAGRRAKPGLKIGVCGEHGGDPAS
                     IAFFEQTGLDYVSCSPFRVPLARLAAARAALARA"
     gene            2908..3651
                     /locus_tag="VFM00_05390"
     CDS             2908..3651
                     /locus_tag="VFM00_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7497713.1"
                     /translation="MIVDAVIPALDEEASIGGVLRSLPAGLLRRVVVCDNGSRDHTAE
                     EARSRGALVVREERRGYGSACLRALAALRTDPPDAVLFLDADGSDDPSEAPALIEPIA
                     SGRADLVIGSRTLGRREPGALTPQARFGNWLATGLIRLFYGARWTDLGPFRAVRWDAL
                     QRLGMRDPDFGWTVEMQVKAARAGLRGMEVPVRYRRRIGRSKISGTLSGTVRAGVKIL
                     GTIGSDLLRRGPARRAAHDGSSAARGSAP"
     gene            3687..>4683
                     /locus_tag="VFM00_05395"
     CDS             3687..>4683
                     /locus_tag="VFM00_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET7497714.1"
                     /translation="MRLQRPVSAPTLALLVFLAWGACGYTPPPLLRTDVNLQPSSTCR
                     SCHLDITDQWNRSAHSRADRTKSPLFGRMYFYSLKATRGGTMLSCGPCHETASFVNQD
                     FEFVREVSQEGVTCTYCHSVSSPAERGVPPYTLDLSAYQGTIANPVKTNGHPSAYAPY
                     LKGSDFCGACHAYSNQHGVKIADTYGEWKRSRYAKQGVSCQGCHMPGKGGRNSNQGPE
                     RPRVADHSFSVDALVAARPNAATLTLTGARRPGADTLRVFALATNTGWGHSLPTGNDQ
                     NLALIRIRVTGADGKIVWENDPFTEWNVSVFGLILADELGNWPADSWNAVKTLSDR"
BASE COUNT          708 a         1686 c         1615 g          674 t
ORIGIN      
        1 tgagcaagac gatgacgatg ccggagcggg tcctgagccc agccccgcgc aaggcggtgt
       61 acttcttcgg cgacggcaaa gccgaaggcg atgcccgcat gcgcgatctt ctcgggggga
      121 agggcgccgg gctcgcggag atgatgaacg ccggcgtgcc ggtgccgccc ggctacacca
      181 tcacgacgga ggcctgccgc ctcttcttcg aatcgggcgg acgactgccc gagagcatcg
      241 cgcgcgagga gaaggcgatg ctcgcgcgcc tcgagcgcct gctgggacgg cgcctgggcg
      301 atcccgagaa cccgctcctc ctgtcggtgc gctccggctc gccggtctcg atgcccggca
      361 tgatggacac gatcctgaac ctgggcctga atgacgagac cgtcgagggg ctggcgcgga
      421 agacgcggaa cgcgcggttc gcctacgact cctaccgccg cttcatccag atgttcgccg
      481 acgtcgtgct gggcgtgccg cgcgggctcc tcgagggcga gctgaccgcg gccaagcgcc
      541 gcgcgcgcgt gaagagcgac gtggagctgg gcgaggcgga tctccgcccg ctctgcgcgc
      601 gcatgctgcg gatcgtccgc gacgagacgg gcaaggcgtt cccgcaggat gccaaggagc
      661 agctcgcggc cgcgcgcgac gcggtgttcc ggtcctggca cacggaccgg gccgtgcact
      721 accgcgccct gaacggcatc cccgagaccc tgggcaccgc ggtcaacgtc caggcgatgg
      781 tcttcggcaa cctcggcgcc acgtcggcca ccggcgtcgg cttcacgcgc gatccctcca
      841 ccggagagcc ccgcttctac ggcgagtatc tgatgaacgc gcagggggag gacgtcgtgg
      901 ccgggatccg cacgccccat ccgatcgaga tgatggagaa ggagctgccc gcggcctacg
      961 cccagctccg gcagatcacc gagcgcctcg agcgccatta ccgcgacgtg caggacttcg
     1021 agttcaccgt ggaggagggg cgcctcttca tgctccagac ccgccgcggc gctcggaccg
     1081 gcatcgccgc gctccggatc gcggtggaca tggtggacga gggactcctc gggaagcgcg
     1141 aggcgctgga gcgcgtggag cccgaccatc tggaccaggt gctccatccc gtgctggacc
     1201 ccgccgcgcg gaagaagcac gcgctgctcg cgaagggact tccggcctcg cccggcgcct
     1261 cggccggcgc gatcgtcttc acgtcggagg aggcagtggc gcggggcaag aaagagcgcg
     1321 tgctcctcgt ccgcgcggag acctcgcccg acgacatctc cggcatgaac gcggcgcagg
     1381 ggatcctgac cgccacgggc ggcatgacct cccacgcggc ggtggtcgcg cgcgggatgg
     1441 gaaagtgctg cgtggccggg tgcaccgcgc tcgtgatcga cgaggacaag cgcacgctcc
     1501 ggatcggcag ccgcacgttc cccgagggcg ctttcctctc cctcgacggc tcgaccggcg
     1561 aagtgctgga gggggacgtc cccacctccg actcggaggt ggtccgcgtg ctgaccgggc
     1621 gcatgacccc cgcgcaggcg ccgctctacc agcgcttcga gcggctcatg gcctggtgcg
     1681 acgaggtgcg ccggctgcgc gtgcgcgcca acgccgacat cccgcgcgac gccaaggtgg
     1741 ctcgcgccct gggcgccgag gggatcgggc tctgccgcac cgagcacatg ttcttcgcgg
     1801 aagaccggat cccgcacgtg gtcacgatga ttttgaacgc caaggaagcc cggcaggcgc
     1861 gccttcggat cgcgcggatc gagtccgacc tggccgaggc cgggaaatcc gaccggccgc
     1921 ggctggcgaa agagctggcg cgcgcgaaga aggaagggaa gggcgcgatc caggcgttcg
     1981 acggcgcctt ggcgaagctc ctcccgctcc agcgtgcgga tttcaaggga ctcttcctgg
     2041 cgatggaggg gacgccggtc accatccgga cgctcgaccc gcccctccac gagttcctcc
     2101 ccaagcggga gcagctcctg gccgagatgg cctcgctacg ccgcgcgaag aaggacgatc
     2161 cccggagagc gcgcctggaa cgcaccctgg aacgcgtgga ggagctgcac gagttcaacc
     2221 cgatgctggg tttccggggc tgccggctcg gcatcctcca tcccgagatc acgcgcatgc
     2281 aggcccgagc catcttcgag gcggccgccc aggtggccaa ggaagggctt cccgtgcggc
     2341 cggagatcat gatcccgctg gtcggggacc gggaagagct gcggcgccag cgcgccgagg
     2401 tggagtcggt cgccaaggaa gtcatggcca agctgggaac caccatcccc tacacgatcg
     2461 ggaccatgat cgagatcccg cgcgccgcga tgctcgccga cgagatcgcc gcgcacgccg
     2521 acttcttctc tttcggaacg aacgatctca cgcagatgtc gttcggcttc tcgcgcgacg
     2581 attgcggccg gttcctgccc gaatacgtcg aggccgggat ccttccgatc gacccgttcg
     2641 tctccctcga ccagcgcggc gtgggggtga tgatccagcg cgcctgcttc gcgggacgcc
     2701 gcgccaaacc cggcctcaag atcggggtct gtggggagca cggcggcgat cccgcttcca
     2761 tcgcgttctt cgagcagacc gggctggatt acgtctcgtg cagccccttc cgcgtgccgc
     2821 tcgcccggct cgcggccgcg cgcgccgcgc tcgcgcgggc ctgacggttc gccagcttcg
     2881 agccggcagg gcgttccggc gcgcgcgatg atcgtggacg ccgtcattcc ggccctggac
     2941 gaggaggcct cgatcggagg cgtcctgcga tcgctcccgg caggtctcct ccgccgcgtc
     3001 gtcgtgtgcg acaacggttc gcgcgaccac accgccgagg aagcccgctc ccgcggcgcg
     3061 ctggtggtgc gggaggagcg ccgcggctac ggctcggcct gcctcagggc cctggcggcc
     3121 cttcggaccg acccgcccga cgccgtcctc tttctcgatg ccgacggaag cgacgatccc
     3181 tcggaagcgc cggcgctgat cgagccgatc gcctccggtc gcgccgatct cgtgatcggc
     3241 tcgcgcacgc tgggccgccg cgagcccggc gcgctcaccc cccaggcgcg cttcggaaac
     3301 tggctcgcga cggggctgat ccgcctcttc tacggcgcgc ggtggaccga cctgggtccc
     3361 tttcgcgcgg tgcggtggga cgcgctccag cggctgggca tgcgggaccc ggatttcggc
     3421 tggaccgtcg agatgcaggt caaggccgcg cgggccgggc tccgggggat ggaggtgccg
     3481 gtgcgctacc gccggcgaat cggccgttcg aagatctcgg gcaccctttc cgggaccgtg
     3541 cgggcgggag tcaagatcct gggcacgatc ggatccgacc tcctccgccg cggaccggcg
     3601 cgccgtgccg cccacgacgg atcctcggca gcgcgcggga gcgcgccttg accccctccg
     3661 ccgcaccgcg ataccatcga gccatcttgc gactccaacg tcccgtttcc gcgccgaccc
     3721 tagcccttct tgtgttcctg gcctggggcg cctgcggcta cacgccgccg ccgctcttgc
     3781 gcaccgacgt caacctgcag ccctcgtcca cctgccgttc gtgccatctg gacatcaccg
     3841 accagtggaa ccgctcggcc cactccaggg ccgaccgcac gaagagtcct ctcttcgggc
     3901 gcatgtactt ctattcgctc aaggccacgc gcggcggaac gatgctttcg tgcggcccgt
     3961 gccacgaaac cgccagcttc gtgaaccagg acttcgaatt cgtccgcgag gtctcgcagg
     4021 agggagtgac ctgcacctat tgccactccg tctccagccc ggccgaacgc ggcgtgcctc
     4081 cgtacacgct cgacctctcc gcctaccagg ggacgatcgc caatccggtc aagaccaacg
     4141 ggcacccctc cgcgtacgcg ccgtacctca aaggctccga cttctgcggc gcctgccatg
     4201 cctactccaa ccagcacggc gtgaagatcg ccgacaccta cggcgagtgg aagcgctcgc
     4261 gatacgcgaa gcagggggtg agctgccagg gatgccacat gccgggcaag ggagggcgca
     4321 actccaacca gggtcccgag cgcccgcgcg tggccgatca ctccttcagc gtcgacgcgc
     4381 tcgtggccgc gcggcccaac gccgcgacgc tcacgctgac gggcgcgcgc cgccccggcg
     4441 ccgacacgct ccgcgtcttc gccctggcga cgaacacggg atggggccac tcgcttccga
     4501 ccggaaacga ccagaatctc gcgctgatcc gcatccgcgt gacgggagcg gacgggaaga
     4561 tcgtctggga gaacgatccc ttcaccgagt ggaacgtctc ggtgttcggg ctgatccttg
     4621 ccgacgagct gggcaattgg ccggccgaca gctggaacgc ggtgaagacg ctgagcgacc
     4681 ggc
//
LOCUS       DASSLZ010000144         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1571163, whole genome shotgun sequence.
ACCESSION   DASSLZ010000144 DASSLZ010000000
VERSION     DASSLZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571163"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VFM00_05400"
     CDS             <1..796
                     /locus_tag="VFM00_05400"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7497715.1"
                     /translation="TQSGTYTVTFTGNDGNGGTGTATTVITVNDVTGGTFTATAKLVG
                     NFNPHRRFLCFHVTATGNSFDVGTTDLSSLVLNYNGATLQALDRQTHLESSCETEVCE
                     DCGTSEATDTTNCTTQLHVCFLTSDVATLLGGNIPDNLSAITITGNLTGGGTFTATLG
                     TFRVAGQGAGNAKGLFKSKAKPNPLNPKTDLTFSLPTAAHVRVSVFDARGRLVKTLLD
                     GNRPAGENVVPWDGTDDRGGHVSSGVFFFRLEALGSKEMVRVTVLK"
     gene            complement(876..1784)
                     /locus_tag="VFM00_05405"
     CDS             complement(876..1784)
                     /locus_tag="VFM00_05405"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HET7497716.1"
                     /translation="MAETVGVVGAGALGSLLALKLARAGHAVIALARSEARRSALAAD
                     APHGLTVERDPAALRSASLLFLCVKARDTEAAARGLSALAPRFPGVCSLQNGWDHMTL
                     LEKALPGAPLIAGATSLGAYFDETGFLQASLEGQTMLAPWGDTEARWAEYAATVLESA
                     GLRAEARGNARQILWRKLALNAAVNPLTALLDRENGALLQIPALGRLARAAAREAAAV
                     GVRLGYLPEGYDPAPRLERLLGETRGNRSSMAEDLARGRPTEADAILGPILRAAREAG
                     METPVIAALAELLRAAEAERGEPPPA"
     gene            1881..2654
                     /locus_tag="VFM00_05410"
     CDS             1881..2654
                     /locus_tag="VFM00_05410"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7497717.1"
                     /translation="MPLRAVVFDYGNTLVSVDPALHSKRTDYADVVAVPGAERMTRHL
                     ESRGLVTREQGMRFLDRFLAIREMNRTMADESGDEIPASMSLVETFRAIGLPEPPSAE
                     IVLAVTRFFSAEEELLQEIPGAAATLRALEERGIALALLSNATDGDSIRRGVERFGWG
                     GVFWPLIVSSDIGVRKPRAEAFRAVLRDWPFAPSEIAVVGDSLRHDVGGAQALGLGAY
                     HFTWIPNPWDPVYLGRIVPGASAATHPDLLRLLLEAAGG"
     gene            complement(2651..3304)
                     /locus_tag="VFM00_05415"
     CDS             complement(2651..3304)
                     /locus_tag="VFM00_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497718.1"
                     /translation="MRFQRLLRAALLAVALAVTGAAFFSGCASNSGLVNMWRDPAYNA
                     PPMQKMLVVAIVRNSANRRILEDAFGKQLAKRGVDAVPSYSRFPDAPPDTMQILDAVG
                     QGNYDGVLVATRLRPERTTSYVPGYVTTQPAYRVSPWSGRYRTYWVDVMHPGYVEENI
                     TVRHDVELWSMREQGNLVWSAVGETVNPSSPTEVTHDIAHNVVPELERQGFIPRKRT"
     gene            3564..>3710
                     /locus_tag="VFM00_05420"
     CDS             3564..>3710
                     /locus_tag="VFM00_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HET7497719.1"
                     /translation="MLARVTSCATVGIEGLLVEVEADLGVGLPTFTIVGLPDAAVRES
                     QERVL"
BASE COUNT          542 a         1291 c         1279 g          598 t
ORIGIN      
        1 gacccagtcc ggaacgtata cggtgacctt caccggcaat gacggcaacg gcggcaccgg
       61 gacggcgacg accgtgatca cggtcaacga cgtcacgggc ggcacgttca ccgcgaccgc
      121 caagctggtg ggcaatttca atccgcaccg ccgcttcctc tgcttccacg tcacggcgac
      181 gggcaactcg ttcgatgtgg gaaccacgga tctttcgagc ctcgttctga actacaacgg
      241 tgcgacgctg caggcgctgg accggcagac gcacctggag tccagctgcg agaccgaggt
      301 ttgtgaggac tgcggaacgt ccgaagcgac cgacaccacg aactgcacga cgcagctcca
      361 cgtctgcttc ctgacgtcgg acgtcgcgac gcttctcggc gggaacattc ccgacaacct
      421 ctccgcgatc acgatcaccg gcaatctcac gggcggcggt acgttcacgg ccaccctggg
      481 caccttccgc gtggccggac agggcgccgg caatgccaag ggattgttca agtccaaggc
      541 gaagccgaat ccgctcaatc ccaagaccga cctgaccttc tcgcttccga cggcggccca
      601 cgtgcgcgtc agcgtcttcg atgctcgcgg ccgcctggtg aagaccctgc tggacggcaa
      661 tcgaccggcc ggtgagaacg tcgtgccgtg ggacggcacg gacgaccggg gcggtcacgt
      721 ttcttcgggt gtcttcttct tccggctgga agcgctcggc tcgaaggaga tggtccgggt
      781 gaccgttctc aagtagggga gcatcgcagc aggcatacga acgggcgggg cgcttcggcg
      841 ctccgcccgt cttcgtttcc cgccctcggc gctgcctacg ctggaggcgg ttcgccgcgc
      901 tccgcttcgg ccgcacggag cagctcggcc agcgccgcga tgacgggcgt ctccatcccc
      961 gcttcgcgtg cggcacgaag gatgggcccc aggatcgcgt cggcctccgt cggccgccct
     1021 cgcgccagat cctcggccat cgaagagcgg ttgccgcggg tctctcccag gaggcgctcc
     1081 aggcgcggcg ccggatcata gccttcggga aggtagccga gccgcacgcc gacggcggcg
     1141 gcctcgcggg cggcggcgcg ggccagccgc ccgagcgccg gaatttggag cagcgcgccg
     1201 ttctcgcgat cgaggagggc cgtcagcggg ttcaccgccg cgttgagcgc gagctttcgc
     1261 cagaggatct ggcgcgcgtt cccgcgcgcc tcggcgcgca gccccgcgct ctccagcacg
     1321 gtggccgcgt attcggccca ccgcgcttcg gtgtcgcccc agggcgcgag catcgtctgg
     1381 ccttcgagcg acgcctggag gaatcccgtc tcgtcgaaat aggcgcccag cgaggtcgca
     1441 ccggcgatca gaggcgcgcc tgggagcgcc ttctcgagaa gggtcatgtg atcccacccg
     1501 ttctggagcg agcagacccc cgggaagcga ggcgcgagcg ccgacaggcc gcgagcggcc
     1561 gcttcggtgt cgcgggcttt cacgcagagg aaaagaaggg aagcggaacg gagcgccgcg
     1621 gggtcgcgtt cgaccgtgag gccgtgcggc gcgtcggcgg ccagcgccga ccgccgcgct
     1681 tcggagcggg ccagggcgat gaccgcgtgc ccggcgcgcg cgagcttcaa ggccagcagc
     1741 gatcccaggg cccccgcgcc cacgacgccg accgtttccg ccatgcgccg atcccccttg
     1801 gttcgacccc ttcgccgggg ttagactgcc cggcccgagc ggttccgatg cgcgattcta
     1861 ccccgaattc gagctgagcc attccccttc gagcggtcgt cttcgactac ggcaacacgc
     1921 tggtgagcgt cgatccggcc cttcactcca agcggaccga ctatgccgac gtcgtggccg
     1981 tccccggcgc cgagcgaatg acccggcatc tcgagtcccg gggcctcgtg acgcgggagc
     2041 agggaatgcg cttcctggat cgctttctcg cgatccgcga gatgaaccga acgatggcgg
     2101 acgagtcggg cgatgagatt ccggcctcga tgtcgctcgt ggagaccttc cgcgccatcg
     2161 ggctgccgga gcccccaagc gcggagatcg tgctcgcggt cacgcgcttc ttcagcgcgg
     2221 aggaggagtt gctccaggag atacccggag ccgcggcgac gctgcgcgct ctagaagagc
     2281 ggggaatcgc gctcgcgctc ctgtcgaacg cgacggacgg cgattccatc cggcgtggcg
     2341 tggagcggtt cggctggggc ggtgtctttt ggccgctgat cgtctcgtcc gacatcgggg
     2401 tgcggaagcc gcgcgccgag gcgttccgcg ccgtgctgcg ggactggccg ttcgcgcctt
     2461 cggaaatcgc ggtggtgggc gactccctgc gccacgatgt cggcggcgcc caagcgctgg
     2521 gcctgggcgc ctaccacttc acgtggatcc cgaatccctg ggatcccgtc tatctcggcc
     2581 gcatcgtccc cggcgcttcg gccgcgacgc accccgatct gctgcggctg ttgctggagg
     2641 cggccggcgg ctaagtgcgc tttcgcggaa tgaagccctg ccgctccagc tcgggaacca
     2701 cgttgtgcgc gatgtcgtgg gtcacctcgg tgggcgagga gggattcacc gtctccccca
     2761 cggccgacca tacgagattt ccctgctccc gcatgctcca gagctccacg tcgtgccgca
     2821 cggtgatgtt ctcctcgacg tagcccgggt gcatcacgtc cacccagtag gtgcggtagc
     2881 ggcccgacca ggggctgacg cgataggcgg gctgcgtcgt gacgtagccc ggaacgtagc
     2941 tcgtggtccg ttccggccgc aggcgcgtcg cgaccagcac gccgtcatag ttgccctggc
     3001 ccacggcgtc caggatctgc atcgtgtcgg gcggcgcgtc cgggaagcgg gagtaggacg
     3061 gcacggcgtc caccccgcgc ttggccagct gtttgccgaa cgcgtcctcc aggatccgcc
     3121 ggttcgcgga attccggacg atggcgacga ccagcatctt ctgcatcggc ggggcgttgt
     3181 acgcggggtc ccgccacatg ttgacgaggc ccgaattgga ggcgcagccg ctgaagaacg
     3241 cggcgcccgt tacggcgagc gccacggcga gcaacgccgc gcggagcagt cgttgaaaac
     3301 gcatggtcat tcctctccca ggtagggtgc tggatcgatc gggcccgccg gagagggtgg
     3361 cggaggccgg atccgggttc acgattcggt tgccggctgc cggcgggaat tccgccggtg
     3421 cattccttcc agtatagggt ctcttccggt ggtgtgatag cgtcgtggtt cccccgccgg
     3481 ctccgccggc ctccgtcccg cgccgtcccg agcggcgtgc ccccgaaccg atccacgtcc
     3541 gcgctccccc gaaaggcgac atcatgctcg ctcgcgtcac gtcctgcgcg accgtcggca
     3601 tcgagggact tctggtcgag gtggaagcgg acctcggcgt cggccttccc accttcacga
     3661 tcgtcgggct tcccgacgcc gccgtgcgtg agagccagga gcgcgtgctc
//
LOCUS       DASSLZ010000145         8806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1690801, whole genome shotgun sequence.
ACCESSION   DASSLZ010000145 DASSLZ010000000
VERSION     DASSLZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8806
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690801"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VFM00_05425"
     CDS             <1..703
                     /locus_tag="VFM00_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497720.1"
                     /translation="GHERTYHPGEINTRIADVVVINKVGTTDYANVEAVRASVQQLNP
                     NAVIVDGASPISVDRPDRIRDRRALVIEDGPTLTHGEMKYGAGVIAAKRHGAREIVDP
                     RPWLVGEIKDTFRQYPGIGALLPAMGYSGNQLRDMERTINACECDVVVIATPIDLRRL
                     VDIKHPTVRVGYELEEIGRPKLEDVIGEFLAARSAKPAARKSPKPARSGKGRVARAAN
                     KPPVPTGRSRGRGKR"
     gene            700..1836
                     /gene="sucC"
                     /locus_tag="VFM00_05430"
     CDS             700..1836
                     /gene="sucC"
                     /locus_tag="VFM00_05430"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET7497721.1"
                     /translation="MNLHEFQGKELFRAAGIPVPPGEVARSADDAATIADRLGYPVVV
                     KAQVLIGGRGKAGGVKVVKNRDEVLREAGRILGMDIRGHVVRQVLITPATDIEKEFYA
                     GIVLDRKFETPLLMVSPSGGVDIEEVARMAPEKILRVHLDGRGLPAYRARAAARFLDP
                     RFPVQKQIVPILTRLAALYHDQDASLAEINPLVVSPKGEVWALDAKVVIDDNALDRHA
                     DLAGMRDLGAEDPGEVEAREKGLSYVRLDGTIGCVVNGAGLAMATMDLIQYHGGKPAN
                     FLDIGGSSNPDKVTAAMNILTRDARVRAVLFNIFGGITRCDDVARGLLTALDRIGMKL
                     PIVIRLTGTNEQEAREILSGRGMTALSDMDEAVRAVIARAAEAA"
     gene            1833..2711
                     /gene="sucD"
                     /locus_tag="VFM00_05435"
                     /pseudo
     CDS             1833..2711
                     /gene="sucD"
                     /locus_tag="VFM00_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
     gene            2998..3414
                     /gene="ndk"
                     /locus_tag="VFM00_05440"
     CDS             2998..3414
                     /gene="ndk"
                     /locus_tag="VFM00_05440"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET7497722.1"
                     /translation="MESTLFMIKPDAVLAGKIGLILAEVEKAGFEVAEMRMVRLTPEK
                     ARRFYAVHEGKPFLADLVSFMSSGPAVPCLLRRDNAIKGLRDLIGATDPKEAKPGTIR
                     ALYAESKQNNAVHASDSPESAATEIPFFFDAVGAAR"
     gene            3426..3959
                     /locus_tag="VFM00_05445"
     CDS             3426..3959
                     /locus_tag="VFM00_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET7497723.1"
                     /translation="MQIDLRELAQGQTHLEMDVTGDSVGLLPTEAILDAPIHLALNLD
                     RRHDEIWIRGTAQATAHQECSRCLVEFPEKLELEFDVFCAKVPATNVQGHPALDEEDG
                     GVHFHDGRTLSIDDEIREAVILGLSMKPLCREDCKGLCPQCGEDRNAGPCRCERAVTQ
                     GAAREAAPESGVQPREE"
     gene            3963..4154
                     /gene="rpmF"
                     /locus_tag="VFM00_05450"
     CDS             3963..4154
                     /gene="rpmF"
                     /locus_tag="VFM00_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET7497724.1"
                     /translation="MAVPKRRHSSTRGKKRRTNWKLRMPSRSLCSHCSQPKLPHRVCA
                     HCGYYAGEEVIAREGLRAS"
     gene            4173..5204
                     /gene="plsX"
                     /locus_tag="VFM00_05455"
     CDS             4173..5204
                     /gene="plsX"
                     /locus_tag="VFM00_05455"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET7497725.1"
                     /translation="MRQQGKGPVIGVDAMGGDLAPRVVIEGALEALGEPGASFDVALI
                     GDEREIQAEADRLGVRDRLPRVIHAAEVIEMAESAAQSVRKKRDSSISVAARLHKSGE
                     TDALVSAGNTGAVVAAALFEIGRIAQIHRPAIATVLPTPQGNVVILDVGATSDCKPSH
                     LYQFAVMGSIYARLVLGIERPRVGLLNIGEEAEKGSELYYEAHQLLKRSPIHFVGNVE
                     GKDIILGTADVVVCDGFVGNVLLKFAESVIPSLSTMIKDEISAHPLSMTAGLLLKPAF
                     RRLKRRLDYAEVGGAPLLGVDGTCIIGHGRSNPRAIKNAIKVAARCAEARVADSIRLE
                     LERLTPAAA"
     gene            5216..6208
                     /locus_tag="VFM00_05460"
     CDS             5216..6208
                     /locus_tag="VFM00_05460"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET7497726.1"
                     /translation="MTATPRDVHIVGTGSHTPSRVLTNKDLEQIVDTSDEWITSRTGI
                     KERRISDPTTPSSVLATEAAKKALADAKIDGSALDQIIVGTVTGDRIFPSTACLLQDN
                     LGATKAHAFDVSAACAGFLYGLSLGYNAIRVGAAETVLVVGVETLSRIVNWTDRNTCV
                     LFGDAAGAVVLRSTGAPGGILAICLHSDGSLVHLLEMPAGGSLRPPSHETVDQRLHTI
                     TMSGNDVFKHAVRAMESVAMECLDASGHTPEELDLIIPHQANLRIVDATARRLGLPSE
                     KVFVNLDRYGNTSSASIPLAMDEARRTGRIRAGSLVELVTFGGGFTWAAAMVQW"
     gene            6243..7199
                     /gene="fabD"
                     /locus_tag="VFM00_05465"
     CDS             6243..7199
                     /gene="fabD"
                     /locus_tag="VFM00_05465"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET7497727.1"
                     /translation="MTGPAFLFPGQGSQSVGMGRLLADRYPEAREAFEEADAVLGFSL
                     TKLCFEGPEAELTRTENTQPALLATSVAAFRALSTKGIAPAAAAGHSAGEYAAHVAAG
                     TFSYADGLRLIRARGEAMAHAGDERPGAMAAVLGLAAEQIAEVLRAVDAPRDLAAANY
                     NSPGQVVLSGTPEALARATDEAKRLGAKRVVPLTVSAAFHSPLMEAAARGLALALDQV
                     AFARPRFPVYANVSAKPVREPEEARATLKEQLLQPVLWEQTMRALIADGTRRFVEVGN
                     GRVLRGLAKSVDRELALFGSQDPDEVEAASAPADAAPAPGSR"
     gene            7196..7942
                     /gene="fabG"
                     /locus_tag="VFM00_05470"
     CDS             7196..7942
                     /gene="fabG"
                     /locus_tag="VFM00_05470"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET7497728.1"
                     /translation="MSGRFDTRVAVVTGGAKGIGLAVSRRFACEGAKVVLTGRDTAAL
                     ESAAAEIRAMGTEALPLAADAAREGEADRVCAATLEAFGRADILVNNAGVTRDGLLLR
                     MSDEDWDLVLETNLKGAFRMIRAFARPMVKQRWGRIVNVSSVIGMIGNAGQANYAASK
                     AGLLGLTKAVAKELASRHITVNAVAPGFIETAMTEGLGEKVREGLQSQIPLGRLGSPD
                     DVAHAVAFLCSEEAGYVTGQVLPVDGGMVM"
     gene            7993..8241
                     /gene="acpP"
                     /locus_tag="VFM00_05475"
     CDS             7993..8241
                     /gene="acpP"
                     /locus_tag="VFM00_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009142622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7497729.1"
                     /translation="MASFSEERVKQIIVDQLGVAPEQVTPEASFIDDLGADSLDTVEL
                     VMALEEAFDIEIPDEDAEKMTTVADAIKYLESHVPKNA"
     gene            8369..>8806
                     /locus_tag="VFM00_05480"
     CDS             8369..>8806
                     /locus_tag="VFM00_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006036553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET7497730.1"
                     /translation="MVRRAVEPRRVVVTGMGVISPLGSDLASFWKRLTAGESGIGPVT
                     RFDITQYDTRIAGEVKDFRAEDYMDRKDARRADLFVQYAVAGTRKAVAQANIQDGNVD
                     PNRYGVVIGSGIGGIATFEDQHKNLMEKGPSRVSPFFIPMMISD"
BASE COUNT         1452 a         2937 c         3186 g         1231 t
ORIGIN      
        1 cgggcacgag cgcacgtacc atcctggcga gatcaacacg cggatcgccg acgtcgtcgt
       61 catcaacaag gtgggcacca ccgattacgc gaacgtggaa gcggtgcgcg cctcggtgca
      121 gcagctgaat cccaacgccg tgatcgtgga cggcgcctcc cccatctccg tggaccggcc
      181 cgaccggatc cgcgaccgcc gcgcgctggt gatcgaggac ggcccgacgc tcacccacgg
      241 cgagatgaag tatggagcgg gcgtcatcgc ggccaagcgg cacggcgcgc gcgagatcgt
      301 cgatccgcgt ccctggctgg tcggcgagat caaggacacc ttccgccagt atccggggat
      361 cggcgcgctc ctccccgcca tgggctactc gggcaaccag ctccgcgaca tggagcggac
      421 gatcaacgcc tgcgagtgcg acgtggtcgt gatcgcgacg ccgatcgacc tgcggcgcct
      481 cgtcgacatc aagcatccga ccgttcgggt cggctacgag ctggaggaga tcgggcggcc
      541 gaagctggag gacgtgatcg gcgaattcct cgcggcccgc tcagccaagc ccgcggcgcg
      601 caagagtccg aagccggcgc gttccggcaa ggggcgggtg gcccgtgcgg cgaacaagcc
      661 gccggtgccg accggccgct cccgcgggcg ggggaagcga tgaacctcca cgagttccag
      721 ggcaaggagc tgttccgcgc ggctggaatt cccgtcccgc cgggcgaggt ggcgcgctcg
      781 gccgacgacg ccgcgacgat cgccgaccgt ctcggctacc ccgtcgtggt gaaagcccag
      841 gtcctgatcg gcggacgggg caaggcgggc ggcgtgaagg tggtgaagaa ccgggacgag
      901 gtgctgcgcg aggcggggcg gatcctcggc atggacatcc gcggacacgt cgtgcggcag
      961 gtgctgatca cgcccgccac cgacatcgag aaggagttct acgcgggcat cgtgctggac
     1021 cggaagttcg agacgcccct cctcatggtc agcccctcgg gcggcgtgga catcgaggag
     1081 gtcgcgcgca tggcgcccga gaagatcctg cgcgtccatc tcgacggccg cgggctcccg
     1141 gcgtaccggg cccgggccgc ggcgcgcttc ctcgacccgc gcttccccgt gcagaagcag
     1201 atcgttccga tcctgacccg gctggccgcg ctgtaccacg accaggacgc gtcgctcgcc
     1261 gagatcaatc cgctcgtggt ctccccgaag ggggaggtct gggcgctgga cgccaaggtg
     1321 gtcatcgacg acaacgctct cgaccgccac gccgatctcg ccgggatgcg cgacctgggg
     1381 gcggaggatc cgggcgaggt ggaggcgcgc gagaaggggc tctcctacgt ccggctcgac
     1441 ggcacgatcg gctgcgtcgt gaacggggcc gggctcgcga tggcgacgat ggacctgatc
     1501 cagtaccacg gcggcaagcc ggccaacttc ctggacatcg gcggctcctc caatccggac
     1561 aaggtgaccg ccgcgatgaa catcctgacg cgggacgcgc gcgtgcgcgc ggtcctgttc
     1621 aacatcttcg ggggcatcac gcgctgcgac gacgtggcgc gggggctcct gaccgcgctc
     1681 gaccggatcg gcatgaagct gccgatcgtg atccgcctca cgggaacgaa cgagcaggaa
     1741 gcgcgggaaa tcctgagcgg ccgcggcatg acggccctga gcgacatgga cgaggcggtc
     1801 cgggccgtga tcgcgcgcgc ggcggaggcg gcatgagcat cctcatcgat cgcagctccc
     1861 gcgtgctggt gcagggcatc acgggtcggg acggctcctt ccacgccaag cagatgcgcg
     1921 actacggaac gcgggtcgtc gccggggtga ctcccggcaa gggcggcacc gacgtggacg
     1981 gcatcccggt cttcgattcg gtggaggagg cggtccagaa gaccaaggcc gacgtctcgg
     2041 tcatctacgt tccggcgtcc ctggcccagg acgcgatcta cgaggcgacc gacgccgagg
     2101 tgccgctcgt ggtctgcatc accgagggga tcccggcgcg cgacatgatc gagatcgccg
     2161 cctacctgaa cggccgctcg acgcggctga tcggacccaa ctgcccgggg ctcatctcgc
     2221 cgggacagtg caaggtgggg atcatgcccg ggttcatcca cgcgccgggg cggatcggcc
     2281 tcgtcagccg gagcggaacg ctcacctacg aagtggtctg gcagctcacg cgcgccaaga
     2341 tggggcagtc gacctgcatc ggcatcgggg gcgacccgat catcggcacg cgcttcaccg
     2401 acgcgctcgc cctcttcgag ggggacgaca agaccgacgc cgtcgtgctg atcggcgaga
     2461 tcggcggcgc ggacgaggaa gaggcggccg cgctcatcga gtccaccatg acgaagccgg
     2521 tcgtggcgtt catcgcgggg cagaccgcgc ctcccgggaa gcgcatgggg cacgccgggg
     2581 ccatcgtctc gggcggatcg ggtacggcgc aggagaagat cgcgcggttc gagcgcgcgg
     2641 gggtgccggt cgcgaaggtc ccgtccgaca tcccgcggct ggtgcaggcg gcgctggagc
     2701 gcggtcggcc caagctgcgc gtggtggccg gcggagcggg aggcgccgcg gccgcgagga
     2761 agaaagcggc gccgaagaag cgcgccgtgt cgcgcccggt cgcgaagccc gcggcgaagc
     2821 gcggggcgac gaagaagggg acgggagcgc gcccggcggc caagcgacgc gccaagccgg
     2881 cacgggcgaa atcgcgtcac tgatcggaac ggaactgttc gaggcgctgc ctggagcgat
     2941 ccgggggcgc ctcagtcttg tcgcgcgccg cgcgcgcgac gacatgggag gaagcgcgtg
     3001 gaatcgacgc tgttcatgat caagccggac gcggtcttag cggggaagat cgggctgatc
     3061 ctggccgagg tggagaaggc gggattcgag gtcgcggaga tgcgcatggt ccggctcacg
     3121 cccgagaaag cgcgccgctt ctacgccgtg cacgagggga agccgttcct ggccgacctg
     3181 gtgtcgttca tgtcgtcggg gccggcggtg ccgtgcctct tgcgccgcga caacgcgatc
     3241 aaggggctgc gcgacctgat cggcgctacc gatcccaagg aagcgaagcc ggggacgatc
     3301 cgcgccctct acgcggagag caagcagaac aacgcggtcc acgcctccga ctcgcccgag
     3361 tcggccgcca ccgagatccc gttctttttc gacgccgtgg gagccgcccg ctagtctaaa
     3421 gacccatgca gatcgacctg agagagctgg cccagggcca gacccatctg gaaatggacg
     3481 ttacggggga ctccgtcggg ctgttgccga cggaggcgat cctggacgcg cccatccacc
     3541 tggctctgaa cctggaccgg cgccacgacg agatatggat ccgcgggacg gcgcaagcca
     3601 cggcgcacca ggaatgtagc cgctgcctgg tcgagtttcc ggagaaactc gaattggagt
     3661 tcgacgtctt ctgtgctaag gtgccggcca cgaacgtcca gggccatccg gcgttggatg
     3721 aagaagatgg cggggttcac ttccacgacg gccggaccct ttccatcgac gacgagatcc
     3781 gggaagcggt gatcctcggt ctctcgatga agccgctctg ccgcgaggac tgcaagggcc
     3841 tctgcccgca gtgcggcgag gaccgtaacg ccggaccctg ccgctgcgaa cgcgccgtca
     3901 cccagggcgc ggcccgggag gccgcaccgg aatccggcgt ccagccacga gaggaatgaa
     3961 tcatggcggt acccaagaga aggcattcga gtacccgcgg caagaagcgc cggacgaact
     4021 ggaagcttcg catgccgtcg cggtcgctct gttcccactg cagccagccc aagcttcctc
     4081 atcgggtctg cgcgcactgc ggctactacg ccggcgaaga ggtgatcgcg cgggagggtc
     4141 tccgggcttc gtaggcatcc acgccaacga ccatgcgcca gcaagggaag ggcccggtca
     4201 tcggggtgga tgccatggga ggcgacctgg ccccccgcgt cgtgatcgag ggcgcgctcg
     4261 aggcgctcgg cgagccgggc gcctcgttcg acgtggccct gatcggtgac gagcgcgaga
     4321 tccaggccga ggccgaccgc ctgggcgtcc gcgaccgcct tcctcgcgtg atccacgcgg
     4381 ccgaagtgat cgagatggcc gaatccgcgg cgcagagcgt ccgcaagaag cgcgattcct
     4441 cgatcagcgt cgcggcccgc ctgcacaaga gcggcgagac cgacgcgctc gtttccgcgg
     4501 ggaacaccgg cgcggtcgtc gccgcggccc tcttcgagat cggtcgcatc gcgcagatcc
     4561 accgccccgc catcgccacc gtgctgccga ccccccaggg gaacgtcgtg atcctggacg
     4621 tgggcgccac ctccgactgc aagccgtccc acctctacca gttcgccgtc atgggcagca
     4681 tctacgcccg cctcgtactc gggatcgagc gccctcgggt cggcctgctc aacatcggcg
     4741 aagaggcgga gaaggggagc gagctctact acgaggcgca ccagctcctg aagcggagcc
     4801 cgatccactt cgtcggaaac gtcgagggga aggacatcat cctgggcacg gcggacgtgg
     4861 tcgtgtgcga cggcttcgtc ggcaacgtgc tgctcaagtt cgcggagagc gtgatcccca
     4921 gcctctccac catgatcaag gacgagatca gcgcgcatcc gctgagcatg acggcgggtc
     4981 tgttgctgaa gcccgccttc cggcggctga agcggcggct ggactacgcc gaggtcggag
     5041 gcgcgccgct tctgggcgtg gacggcacct gcatcatcgg ccacggccgc tccaacccgc
     5101 gcgcgatcaa gaacgccatc aaggtggcgg cgcgctgcgc cgaagcgcgc gtggccgact
     5161 cgatccgact ggagctggag cgcctgactc cggcggccgc ctgatccccg atcccatgac
     5221 cgcgaccccg cgcgacgtcc acattgtagg caccgggtcc cacaccccct cgcgcgtcct
     5281 cacgaacaag gacttagagc agatcgtcga cacgtccgac gagtggatca ccagccgcac
     5341 cggcatcaag gagcggcgga tctccgatcc gacgacgccc tcgtcggtgc tcgccaccga
     5401 ggccgcgaag aaggcgctcg ccgacgccaa gatcgacggc tcggcgctgg accagatcat
     5461 cgtcggcacc gtgacggggg accgcatctt cccctcgacc gcctgcctct tgcaggacaa
     5521 cctgggcgcg accaaggcgc acgctttcga cgtctcggcc gcctgcgccg gatttctgta
     5581 cgggctctcg ctcgggtaca acgccatccg cgtcggcgcc gccgagacgg tgctggtcgt
     5641 cggcgtggag accctctccc ggatcgtgaa ctggaccgac cggaacacct gcgtgctctt
     5701 cggggacgcg gcgggcgccg tggtgctgcg ctccaccgga gcgccgggcg ggatcctcgc
     5761 gatctgcctc cacagcgacg gatcgctggt ccacctgctc gagatgcccg cgggcggctc
     5821 cctgcgtccg ccgtcgcacg agaccgtgga ccagcgcctt cacaccatca cgatgagcgg
     5881 caacgacgtc ttcaagcacg ccgtccgcgc catggaatcg gtggcgatgg agtgcctgga
     5941 cgcgtcgggg cacacgcccg aggagctgga cctgatcatt ccgcaccagg ccaatctgcg
     6001 catcgtcgac gcgacggcgc gaaggctggg ccttccctcc gagaaagtgt tcgtgaacct
     6061 ggatcgctac gggaacacgt cgtcggcctc gatcccgctc gcgatggacg aggcgcggcg
     6121 caccggccgc atccgggccg gcagcctggt cgagctggtc acgttcggcg gcggctttac
     6181 ctgggccgcc gcgatggtgc aatggtgacg gcgcacctgg gccgcgccgc gtgggtgtcg
     6241 cgatgacggg accggcgttc ctctttcccg gccagggctc gcagagcgtc ggcatgggac
     6301 ggctgctggc cgatcgctac cccgaggcgc gcgaagcctt cgaggaggcc gacgccgttc
     6361 tcgggttctc cctcacgaag ctctgtttcg aggggcccga ggcggagctg acccgcaccg
     6421 agaacacgca gccggcgctg ctcgccacga gcgtcgccgc cttccgcgcg ctttcgacga
     6481 agggaatcgc tcccgccgcc gcggcggggc acagcgcggg ggagtacgcc gcccatgtcg
     6541 cggcggggac cttctcctac gccgacgggc tccggctgat ccgggcgcgt ggcgaggcga
     6601 tggcccacgc cggggatgag cgccccggcg ccatggccgc ggtgctgggg cttgcggcgg
     6661 agcagatcgc cgaggtgctc cgcgcggtgg acgcgccgcg ggatctggcg gccgccaact
     6721 acaactcgcc gggacaggtc gtgctctcgg gaacgcccga agcgctcgcc cgcgccaccg
     6781 acgaggcgaa gcggctcggg gcgaagcggg tcgtgccgct caccgtgagc gcggcgttcc
     6841 attcgccgct catggaggcg gcggcgcgcg gcctggcctt ggccctcgac caggtggcgt
     6901 tcgcgcgccc ccgctttccg gtttacgcca atgtctcggc caagccggtg cgcgagcccg
     6961 aagaggcgcg cgcgacgctc aaggagcagc tcctccagcc cgtgctctgg gaacagacga
     7021 tgcgcgcgct gatcgcggac gggacgcgcc ggttcgtcga ggtcggaaac gggcgggtgt
     7081 tgcgcggcct ggcgaagagc gtggaccggg agctcgcgct cttcggctcg caggatccgg
     7141 acgaggtgga ggcggcttcg gcgccggcgg acgcggctcc cgcgccgggg agccgatgag
     7201 cgggcgcttc gacacgcgcg tcgcggtcgt gacgggcggc gccaagggga tcgggctcgc
     7261 ggtgtcgcgg cgtttcgcct gcgagggggc caaggtggtg ctcacggggc gggatacggc
     7321 ggcgctggag tccgcggcgg ctgagatccg cgccatggga acggaggcgc ttcccctcgc
     7381 ggccgacgcc gctcgggaag gtgaagccga cagggtttgc gcggcgaccc tcgaggcgtt
     7441 cggccgggcc gacatcctcg tgaacaacgc gggggtgacc agggacgggc tcctgctccg
     7501 catgagcgac gaggactggg acctggtcct cgagacgaac ttgaagggag cgttccggat
     7561 gatccgcgcc ttcgcccggc cgatggtgaa gcagcgctgg gggcggatcg tgaacgtctc
     7621 gtcggtgatc gggatgatcg gaaacgcggg gcaggcgaac tacgccgcct ccaaagccgg
     7681 cctcctgggg ctgacgaagg ccgttgctaa ggagttggca tcacgtcata ttacggtgaa
     7741 tgcggtcgcg ccgggcttca tcgagaccgc gatgacggag gggctgggag agaaggtcag
     7801 ggagggtctc cagtcgcaga tccccctggg ccggctcggc agccctgacg acgtggcgca
     7861 cgcggtggcc ttcctgtgct ccgaggaggc cggttacgtg acggggcagg tgctgccggt
     7921 cgacggcggc atggtgatgt agcgtcccaa acgcttggga cgaatctaag ctcaggggag
     7981 gtgacccaag gaatggcatc gttcagcgaa gaacgcgtga agcagatcat cgtggatcag
     8041 ttgggcgtcg cgcccgagca ggtgactccc gaggcgtcgt tcatcgacga tctcggagcc
     8101 gactcgctcg acacggtgga gctggtcatg gccctcgagg aggcgttcga catcgagatc
     8161 cccgacgagg acgcggagaa gatgaccacg gtggcggacg ccatcaagta tctggaatcc
     8221 cacgtgccga aaaacgccta ggagtgcgcc taggcgcacg cctggagacc gcccgaatcg
     8281 cgcctgattc ggcggcgcgc ccggtagcag ggcgcggcgg gagcagacca gtgggaatcg
     8341 aagctcagga atgggagaag cggagcgcat ggtgcgtcga gcggtagagc cgaggcgggt
     8401 cgtggtcacg ggcatgggag tcatctcccc gctcgggtcc gacctcgcgt cgttctggaa
     8461 acggctcacg gcgggcgagt cggggatcgg ccccgtcacg cgtttcgaca tcacccaata
     8521 cgacacgcgg atcgcgggcg aagtgaagga cttccgggcc gaggactaca tggaccggaa
     8581 ggacgcgcgc cgcgccgacc tgttcgtcca atacgccgtc gcgggcaccc gaaaggccgt
     8641 cgcgcaggcg aacatccagg acggaaacgt cgatcccaac cgctacggcg tggtgatcgg
     8701 ctcgggcatc ggcggcatcg cgacgttcga ggaccagcac aagaacctca tggaaaaagg
     8761 tccttcccgc gtgagcccct tcttcattcc gatgatgatc tcggac
//
LOCUS       DASSLZ010000146         8564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1189553, whole genome shotgun sequence.
ACCESSION   DASSLZ010000146 DASSLZ010000000
VERSION     DASSLZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8564
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189553"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VFM00_05485"
     CDS             <1..644
                     /locus_tag="VFM00_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HET7497731.1"
                     /translation="EAPDVSVGYLPHSYGLDVTLTVLALDPSWAAEAIRRVERRVVEA
                     VGESLYGFGRDTLADVLGGMLADQGRTLVTAESFTAGAIGATITATPGASRYFLGGVT
                     AYSNRAKETLLGVSGTTLARHGAVSAETAQEMASGARERFGADLAVSSTGIAGPDGGT
                     PEKPVGLVYLGLATAEGAQAMRLQLTGTREEIVVRSSALALDLVRRHMAHAPA"
     gene            641..1228
                     /gene="thpR"
                     /locus_tag="VFM00_05490"
     CDS             641..1228
                     /gene="thpR"
                     /locus_tag="VFM00_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET7497732.1"
                     /translation="MTAGLTRTFVALLIPDSWIAYVRGLGQALASRSAGLSWVKPENA
                     HFTVRFLGDLDDEQLAGVRDSVRRSATGLAAPVARLGRLGAFPRPERPRVLWVGLAQG
                     GPETEALAAVVNDALERDGFGFPDRPFRAHLTLARAREGARGVDALIRTPLAEAPEAE
                     PLHRLAVMKSQLHKSGAMYTVLEEIRLQSPGSQAG"
     gene            1386..2459
                     /gene="recA"
                     /locus_tag="VFM00_05495"
     CDS             1386..2459
                     /gene="recA"
                     /locus_tag="VFM00_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET7497733.1"
                     /translation="MSKERTKALELAVQQIERQFGKGAIMKLGSDSARVQVEAIPTGS
                     IALDAALGVGGIPRGRVIEVYGPESSGKTTLALSIIAQAQKQGGNAVFIDAEHALDAA
                     YAKRLGVDIDALHVSQPDTGEEALEIAEHLVRSGAVDVIVIDSVAALVPKAEIEGEMG
                     DSHMGLQARLMSQALRKLTGTISKSRTTVIFINQIRMQIGVMFGNPETTTGGRALKFY
                     ASIRLDIRRIGSIKDGDNVIGNHVKVKVVKNKVAAPFKEAEFDILYNEGISREGELLD
                     LAVERNLVQKSGTWFSYGEERIGQGRENARLWLREHPDAFAELSVKMREALGLSRGAA
                     AEAEENGSEPKAAAPSGDRRVRI"
     gene            2469..2990
                     /locus_tag="VFM00_05500"
     CDS             2469..2990
                     /locus_tag="VFM00_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HET7497734.1"
                     /translation="MRRPMSPEQAASDDSAKEAALRILARGPRTEREVMDRLIERGYV
                     PDAVERAVERLRRVSLLDDRAYLAWFVRAELGRKPQALRLIETKLARHGLPRPLVAAA
                     EEAIRADGDLAERALDTEEGRAEAAAREVGKKLARRGPEERRRLLQSALLRRGFSWDT
                     IRDLVSERSHPES"
     gene            2998..5667
                     /gene="alaS"
                     /locus_tag="VFM00_05505"
     CDS             2998..5667
                     /gene="alaS"
                     /locus_tag="VFM00_05505"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET7497735.1"
                     /translation="MPQTTNELRRTFLDFYKDRGHPHLPMAPLVPLDDPTLLFTSAGM
                     VPFKSYYASPVAPPTRRAVTVQRCLRLTDIENIGVTVRHCTLFEMLGNFSFGDYFKKE
                     AIEWAWELITVTLGMPKERLFPSVFREDDEAFDLWAKHIGLGAERVTRLDEKDNFWGP
                     AGGSGPCGPCSEIYWDQGPGTGCGRPECAPGCDCERYLEFYNLVFPQYDQAPDGSRKP
                     LPNRGIDTGMGLERLAMILQGVPSIYDNDLLRPIGDAVKRMGSPKASQGDRGRRSARV
                     ITDHVRALVFTFAEGVRPSNEGRGYVARRLLRRAARFGRDLEIHGPFLSRLVPPVVDA
                     MAAYEEYEYLRREQAAVAAAIHEEEARFAETLETGMTRFEETADSLAKAGSRVYPGSA
                     AFMLYDTYGFPIDLTAELARERGLDVDMPAYEAAMDEQRERARKAGRFETRRKTAGPW
                     QELTRGDHSKFVGYDRLAVEDVSIRRLREVPAEGGGPREVEVTLDVTPFYAEGGGQVG
                     DTGWLEAMPGAAPARLRVVDTVKDADLVVHRAEVVEGTPHAGPYRATVDAAKRAATER
                     NHTATHLLHAALRARLGTGVKQAGSLVAPDRLRFDFSYGKALTPEDLHAIESEVNRNL
                     LEDLPVATEVTSLKEAQERGAMALFGEKYGERVRQVIVDGVSRELCGGCHVSRTGEIG
                     YLRIESESAIASGVRRIEAVTGALAYQRADEDRALLREIAHRLGAPREQLAERLRQIQ
                     EEARALRDERAKQSQGALLERVHALIAEAKSRGEAVITARLEAGSVDELRQAGDVVRK
                     ALPGGGALLLAVIDGKLSVAAVVGPQATDRLQADQWVRDAVSVAGGKGGGKKDSALAG
                     AKEPALAGQVLERGAAYAAERMKGAGAPSGSGA"
     gene            5664..6728
                     /locus_tag="VFM00_05510"
     CDS             5664..6728
                     /locus_tag="VFM00_05510"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HET7497736.1"
                     /translation="MKALVLSGGKGTRLRPITHTGAKQLIPVANKPILFYVLEAIRDA
                     GITDVGIVVGDTENEIRAAVGDGSAFGIRADYIRQDQPLGLAHAVKVARGYLGQDPFV
                     MFLGDNLILDGIRTLVDEFRREQPNSEILLARVPHPEEFGVAELEGDHVVRLVEKPKQ
                     PKSDLALVGVYMFDPSIHEAVDAIRPSFRNELEITDAIQHLIDSGKRVRHHVITGWWK
                     DTGKLEDMLEANRMVLSRAKRRVGGEVDAASRVEGEVIVGQGTVVKNSILRGPLIVGA
                     RCHIENSYVGPFTSLYDDVVLRNSEIEHSIVLERCRIEDIPVRIEASLIGKEVRIQRS
                     QMKPTAYRFMVGDSSLVDVV"
     gene            6730..7590
                     /gene="rfbD"
                     /locus_tag="VFM00_05515"
     CDS             6730..7590
                     /gene="rfbD"
                     /locus_tag="VFM00_05515"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET7497737.1"
                     /translation="MRVLVTGANGILGRALRERLTGGDTLYLWGREEADITDEAATRA
                     AARGLDFDTVIHAAAYTNVDGAESDPAMAMAVNRDGTRHVAALARERGAVLVYVSSDY
                     VFDGTKGSPYVEEDPPHPLGVYGATKLAGEEAALDSGAPCLVVRTSWLFGYGGKNFVD
                     TIAAKALRGEPLQVVSDQHGSPTYARDLAHGIEVLLRRGALGIVHVTNAGETTWHGLA
                     VEIARTLGAPVPIAPVSTAHVARLAPRPAYSVLSGARYRALAGTPLPTWEDAVHRHLA
                     ERPSRQGEAA"
     gene            7587..8276
                     /locus_tag="VFM00_05520"
     CDS             7587..8276
                     /locus_tag="VFM00_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET7497738.1"
                     /translation="MKRASHARASSALRARARAKRAPRPDRVRDIFREALELTAAVYA
                     RERGTIERIAAMIADAALSGRTVFWAGNGGSAAEAQHLATEFVVRFVKNRRALPSLSL
                     HADTSVLTAAANDFGFDRVFARQLEALGRRGDVLVALTTSGRSANVLAAVEEARRRGM
                     RVVGLTGAKGAAFARHCDLALVVPSGDTARVQEIHMLVGHVCCDLAEARVLAEDRRAG
                     GRSRPGKRARR"
     gene            8273..>8564
                     /locus_tag="VFM00_05525"
     CDS             8273..>8564
                     /locus_tag="VFM00_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET7497739.1"
                     /translation="MIRDVIRSMRIYQWTKNLVLFAGPVFTLKILDPAFALPSVLGFI
                     AFSLATSGVYVLNDVLDVERDRLHPTKRSRPVASGRLPIGAAWVMAAILLAAG"
BASE COUNT         1281 a         3001 c         3118 g         1164 t
ORIGIN      
        1 cggaggctcc cgacgtgagc gtgggctacc tgccgcactc ctacgggctg gacgtgacgc
       61 tcacggtgct cgcgctcgat ccgtcgtggg cggccgaggc gatccggcgc gtcgagcggc
      121 gcgtggtgga ggcggtgggc gagtcgctct acggctttgg acgggacacc ctggcggacg
      181 tcctgggcgg gatgctggcg gaccaggggc gcaccctggt caccgccgaa tcgttcacgg
      241 ccggggccat cggcgccacg atcacggcga cgccgggcgc ctcgcgctat ttcctgggcg
      301 gggtgaccgc ctactccaac cgcgccaagg agacgctgct cggcgtctcg ggaacgaccc
      361 tcgcgcggca cggcgccgtg agcgccgaga cggcgcagga gatggcctcc ggcgcgcgcg
      421 agcgcttcgg cgccgacctc gccgtgtcga gcaccgggat cgccggcccc gacggcggga
      481 cgccggagaa gccggtcggg ctcgtctacc tggggctcgc cacggccgaa ggagcgcagg
      541 cgatgcgtct ccagctgacc ggcacgcgcg aggagatcgt ggtgcgctcc tcggccctgg
      601 cgctcgacct ggtgcgccgg cacatggcgc acgcgccggc atgaccgcgg gcctgacgcg
      661 cacgttcgta gcgctcctga tccccgattc ctggatcgcg tacgtgcgcg gcctgggcca
      721 agccctcgcc agccgctcgg ccggcctctc ctgggtcaag ccggagaacg cgcacttcac
      781 ggtgcgcttc ctgggcgacc tggacgacga gcaactcgcc ggcgtgcgcg actcggtgcg
      841 ccggagcgct accggtctcg cggcaccggt tgcgcggctc ggccggctgg gcgcgtttcc
      901 gcgtccggag cgcccgcgcg tgctctgggt ggggctcgcg cagggaggac ccgagacgga
      961 ggcgctcgcg gccgtggtga acgacgctct ggagcgcgac ggattcgggt ttcctgacag
     1021 gccgtttcgc gcgcacctga ctctggcgcg cgcccgcgag ggcgcccggg gcgtggacgc
     1081 gctgatccgc acaccactgg ccgaagcgcc cgaagcggag ccgctccatc gtcttgcggt
     1141 catgaaaagt cagctccaca aaagcggtgc catgtatact gttctggagg aaatacgcct
     1201 ccaatcaccg ggctcgcagg caggctgacg tatgtgtggg gaagcctgcg cgagcccact
     1261 cacaacaccg ttcgccccgg cccgaaccag gcgtcccgga tggatcgccg tcaccaaact
     1321 cagggggagt gcacatggca ggcaacgtga ccgctcggga agtgggccca tcgacgacgc
     1381 gtgagatgag caaggaacgc acgaaggcgc tggagctggc cgtgcagcag atcgagcggc
     1441 agttcggaaa gggcgccatc atgaagctgg gctccgattc ggcccgcgtg caggtcgagg
     1501 cgatcccgac ggggtcgatc gcgctggacg ccgcactcgg cgtcggcggg atcccgcggg
     1561 gacgcgtgat cgaggtctac ggccccgagt cctcgggcaa gacgacgctc gcgctgagca
     1621 tcatcgcgca ggcgcagaag cagggcggga acgcggtctt catcgacgcc gagcacgccc
     1681 tggacgccgc gtacgcgaag cggctcggcg tggatatcga cgccctgcac gtctcgcagc
     1741 ccgacaccgg cgaggaggcg ctcgagatcg ccgagcacct cgtccggagc ggtgccgtgg
     1801 acgtgatcgt gatcgactcg gtcgccgcgc tggtgccgaa agccgagatc gagggggaga
     1861 tgggcgactc gcacatgggc ctccaggcgc gcctcatgtc gcaggcgctg cgcaagctga
     1921 ccggcaccat ctccaagtcg cgcaccacgg tcatcttcat caaccagatc cgcatgcaga
     1981 tcggcgtgat gttcggcaat cccgagacca cgaccggcgg gcgcgcgctc aagttctacg
     2041 cctcgatccg cctcgacatc cgccgcatcg gctcgatcaa ggacggcgac aacgtgatcg
     2101 gcaaccacgt gaaggtgaag gtcgtgaaga acaaggtcgc ggcccccttc aaggaagctg
     2161 agttcgacat cctctacaac gaggggatct cgcgcgaggg cgagctgctc gacctggccg
     2221 tggagcgcaa cctcgtccag aagagcggca cctggttcag ctacggcgag gagcggatcg
     2281 ggcaggggcg cgagaacgcg cggctctggc tgcgggagca tcccgacgcc ttcgccgagc
     2341 tctccgtgaa gatgcgggag gcgctcgggc tctcgcgcgg cgccgcggcg gaagcggagg
     2401 agaacggctc cgagccgaag gcggcggctc cttccggcga ccggcgggtc cggatctagg
     2461 acggagccat gcgacgaccg atgtcgcccg agcaggcggc gtcggacgat tcggccaagg
     2521 aagcggcgct ccggattttg gcccgcggcc cgcgcaccga gcgcgaggtg atggaccggc
     2581 tgatcgagcg tggctacgta ccggacgcgg tggagcgcgc cgtggagcgc ctccgccgcg
     2641 tctcgcttct ggacgaccgg gcctatctcg cctggttcgt gcgcgccgag ctcggccgaa
     2701 agccgcaggc cctccgcctg atcgagacga agctggcgcg tcacggcctg ccgcggccgc
     2761 tggtcgccgc cgcggaagag gcgatccgcg ccgatggcga cctggcggag cgggcgctgg
     2821 acaccgagga ggggcgcgcc gaggcggccg cgcgcgaagt gggcaagaag ctcgcgcggc
     2881 gcggcccgga ggagcggcgg cggctcctcc aatcggccct cttgcgccgg ggcttttcct
     2941 gggacaccat ccgggatctc gtgtccgagc gatcccatcc ggagtcatga cttcctgatg
     3001 ccccaaacga cgaacgagct gcgccggacc ttcctcgact tctacaagga ccggggccat
     3061 ccgcatctgc ccatggcccc gctcgtgccg ctggacgacc ccaccctgct cttcacttcg
     3121 gccgggatgg tgccgttcaa gtcctactac gcctcgccgg tagcgccgcc gacgcgccgc
     3181 gcggtcaccg tgcagcgatg cctgcggctc accgacatcg agaacatcgg cgtcaccgtg
     3241 cgccactgca cgctgttcga gatgctcggc aatttctcct tcggcgacta cttcaagaag
     3301 gaagcgatcg agtgggcgtg ggagctgatc accgtgaccc tcggaatgcc gaaggagcgc
     3361 ctcttcccct cggtcttccg ggaggacgac gaggcgttcg acctctgggc caagcacatc
     3421 ggcctgggcg ctgagcgcgt cacgcgcctg gacgagaagg acaacttctg gggaccggcg
     3481 ggcggctcgg gtccctgcgg accctgctcc gagatctact gggaccaggg gccgggcacg
     3541 ggatgcggcc ggcccgagtg cgcgcccgga tgcgactgcg agcgctacct cgagttctac
     3601 aacctggtct ttccgcagta cgaccaggcg cccgacggat cgcggaagcc gctccccaac
     3661 cgcggcatcg acaccgggat ggggctcgag cggctcgcga tgatcctcca gggcgtcccc
     3721 tcgatctacg acaacgacct gctccgtccg atcggcgacg cggtgaagcg gatgggatcg
     3781 cccaaggcgt cgcagggcga ccgcgggcgc cggtccgcgc gcgtcatcac cgaccacgtg
     3841 cgggccctcg tcttcacgtt cgccgagggc gtgcgcccct cgaacgaggg ccgcggctac
     3901 gtggcgcgcc ggctgctgcg ccgcgcggcg cgcttcgggc gcgatctgga gatccacggc
     3961 cccttcctct cgcgcctggt tcctccggtg gtcgacgcca tggccgcgta tgaagagtac
     4021 gagtacctcc gccgcgagca ggcggcggtg gcggcggcga tccacgagga ggaggcgcgc
     4081 ttcgcggaga cgctcgagac gggcatgacc cgcttcgagg agacggccga ttccctcgcg
     4141 aaggcgggaa gccgggtcta tcccggaagc gccgccttca tgctgtacga cacctacggc
     4201 ttcccgatcg acctcacggc ggagctcgcg cgcgagcgcg ggctggacgt cgacatgccg
     4261 gcctacgagg cggccatgga cgaacagcgg gagcgcgcgc gcaaggcggg gcgcttcgag
     4321 acgcgccgga agacggcggg accgtggcag gagctcacgc gcggggatca ttcgaagttc
     4381 gtcggctacg accgtctcgc ggtcgaggac gtctcgatcc ggcgcctccg cgaggtgccc
     4441 gccgagggcg gcgggccgcg cgaggtggag gtcacgctgg acgtcacgcc cttctacgcc
     4501 gaggggggcg gccaggtcgg ggacaccgga tggctcgagg cgatgccggg cgcggccccg
     4561 gcgcggcttc gcgtcgtgga cacggtgaag gacgccgacc tcgtggtcca tcgcgccgag
     4621 gtggtggagg gcaccccgca cgcgggcccc tatcgcgcga ccgtggacgc ggcaaagcgc
     4681 gccgcgaccg agcggaacca cacggcgacg caccttctcc acgccgcgct gcgcgcgcgg
     4741 ctcggcaccg gcgtgaagca ggcggggtcg ctcgtcgccc ccgaccggct ccgcttcgac
     4801 ttcagctacg gcaaggcgct cacgcccgag gacctgcacg cgatcgaatc ggaagtgaac
     4861 cggaacctcc tcgaggatct cccggtcgcg accgaggtca cgtcgctcaa ggaggcgcag
     4921 gagcggggcg cgatggcgct cttcggcgag aaatacggcg agcgcgtgcg gcaggtcatc
     4981 gtggacggcg tgagccgcga gctctgcggc ggctgccacg tgtcgcgcac gggcgagatc
     5041 ggctatctcc ggatcgagtc cgagtcggcc atcgcctccg gcgtgcgccg gatcgaggcg
     5101 gtcacgggcg ccctggccta ccagcgggcc gacgaagacc gggcgttgct tcgcgagatc
     5161 gcccaccgcc tgggcgcgcc ccgggagcag ctcgccgagc ggctgcggca gatccaggag
     5221 gaggcgcggg cgcttcgcga cgagcgggcg aagcagagcc agggcgcgct gctcgagcgc
     5281 gtgcacgcgc tgatcgccga ggcgaagagc cgcggcgagg cggtgatcac ggcgcgcctc
     5341 gaggcgggca gcgtggacga gctgcgccag gcgggcgacg tcgtgcggaa ggcgctgccg
     5401 ggcggcgggg cgctccttct cgccgtcatc gacggcaagc tctccgtggc ggcggtggtc
     5461 ggcccgcagg cgaccgatcg cctccaggcc gaccagtggg tgcgcgacgc cgtgtcagtc
     5521 gcgggcggga agggtggcgg gaagaaggac tcggcgctcg cgggcgccaa ggagcccgcg
     5581 ctcgccggcc aggtgctgga acggggcgcc gcctacgccg ccgagcggat gaagggcgcc
     5641 ggggccccgt cgggttcggg cgcatgaaag cgctcgtcct ctccggcggc aagggaacgc
     5701 ggctccgccc catcacgcac accggcgcca agcagctcat cccggtcgcg aacaagccga
     5761 tcctcttcta cgtgctcgag gcgatccggg acgcggggat caccgacgtc ggcatcgtcg
     5821 tcggcgacac cgaaaacgag atccgggccg cggtcggcga cggatccgcc ttcggcatcc
     5881 gcgccgacta catccgccag gaccagccgc tgggactggc ccacgcggtg aaggtcgcgc
     5941 gcggctacct gggacaggac cccttcgtca tgttcctcgg cgacaacctg atcctggacg
     6001 gcatccgcac cctggtggac gagttccgcc gcgagcagcc gaacagcgag atcctgctcg
     6061 cgcgcgtgcc gcatcccgag gagttcggcg tggccgagct ggagggcgat cacgtcgtgc
     6121 gcctggtgga gaagccgaag cagcccaagt ccgatctcgc gctggtcggc gtctacatgt
     6181 tcgacccctc gatccacgag gcggtggacg cgatccgccc ctccttccgg aacgagctgg
     6241 agatcaccga cgccatccag cacctgatcg acagcggcaa gcgggtccgc caccacgtca
     6301 tcaccggctg gtggaaggac acggggaagc tcgaggacat gctcgaggcg aaccggatgg
     6361 tcctctcccg cgcgaagcgt cgcgtgggcg gcgaggtgga cgccgcctcg cgcgtggagg
     6421 gagaggtgat cgtgggccag gggacggtcg tgaagaactc gatcctccgc ggccccctga
     6481 tcgtcggggc acgctgccac atcgagaact cgtacgtggg acccttcacc tccctctacg
     6541 acgacgtcgt tctccggaac agcgagatcg aacattcgat cgtgctcgag cgctgccgga
     6601 tcgaggacat cccggtccgg atcgaggcct ccctgatcgg gaaggaagtg cggatccagc
     6661 gctcgcagat gaagccgacc gcctaccgct tcatggtcgg cgactccagc ctggtcgacg
     6721 tcgtctagcg tgcgcgtcct ggtcaccggc gcgaacggga tcctgggccg cgcgcttcgc
     6781 gaacggctga ccggcggcga cacgctctat ctctggggac gcgaggaagc cgacatcacc
     6841 gacgaggcgg cgacccgggc ggccgcgcgc gggctcgact tcgacaccgt gatccacgcc
     6901 gccgcctaca ccaacgtgga cggcgccgag agcgatcccg cgatggcgat ggcggtgaac
     6961 cgggacggca cgcgccacgt ggccgcgctg gcgcgtgagc ggggcgccgt gctcgtctac
     7021 gtgagcagcg actacgtctt cgacggcacg aagggatccc cgtacgtcga agaggatccg
     7081 ccccacccgc tcggcgtcta cggcgccacg aagctcgcgg gggaggaagc cgcgctcgat
     7141 tccggcgcgc cctgcctggt ggtccgcacc tcgtggctct tcggctacgg cgggaagaac
     7201 ttcgtcgaca ccatcgcggc caaggccctt cgcggcgagc cgctccaggt ggtgagcgac
     7261 cagcacggct cgcccaccta cgcccgcgac ctggcgcacg gcatcgaggt gctgctccgg
     7321 cgcggtgccc tgggcatcgt gcacgtgacg aacgcggggg agacgacctg gcacgggctg
     7381 gcggtcgaga tcgcccgcac cctgggggcg ccggtgccga tcgcgcccgt ttccaccgcc
     7441 cacgtcgccc gtttagctcc acgccccgcc tactccgtgc tttcgggcgc gcgctatcgc
     7501 gcgctggccg gcaccccgct tcctacctgg gaggacgcgg tgcaccggca cctggcggaa
     7561 cggccgtcgc gccagggaga ggcggcgtga agcgcgcatc ccacgcccgc gcctccagcg
     7621 cccttcgcgc gagagcccgc gccaaacgcg cgccgcgtcc ggaccgcgtc cgggacatct
     7681 tccgcgaggc cctcgagctg accgcggccg tctacgcgcg ggagcgcggg accatcgagc
     7741 ggatcgcggc gatgatcgcc gacgccgcgc tctcgggccg cacggtgttc tgggccggca
     7801 acggcggcag cgccgccgag gcgcagcacc tcgcgaccga gttcgtcgtg cggttcgtga
     7861 agaaccggcg cgcccttccc tcgctctccc tgcacgcgga cacgtcggtg ctgacggccg
     7921 cggccaacga tttcggcttc gacagggtct tcgcccggca gctcgaggcg ctgggacgcc
     7981 gcggggacgt cctcgtggca ctcaccacga gcggacgttc ggcgaacgtg ctcgcggcgg
     8041 tcgaagaggc ccgtcgccgg gggatgcggg tggtcgggct gaccggtgcg aagggcgccg
     8101 ccttcgcgcg gcactgcgac ctggcgctcg tggtcccgag cggcgacacg gcccgggtgc
     8161 aggagatcca catgctcgtc ggccacgtct gctgcgatct cgcggaggcg cgcgtgctcg
     8221 ccgaagatcg ccgcgcgggc ggccggagcc ggccggggaa gcgcgcccgg cgatgatccg
     8281 cgacgtgatc cgctcgatgc ggatctacca gtggaccaag aacctcgtcc tcttcgcggg
     8341 gccggtcttc acgctgaaga tcctggaccc ggccttcgcc ctcccctcgg tgctcggctt
     8401 catcgccttc tccctcgcaa cctccggcgt ctacgtcctg aacgacgtgc tggacgtgga
     8461 gcgcgaccgc ctccatccga cgaagcgctc gcggccggtc gcctcgggac ggctcccgat
     8521 cggcgcggcc tgggtgatgg cggcgatcct gctggccgcg gggc
//
LOCUS       DASSLZ010000147         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_684166, whole genome shotgun sequence.
ACCESSION   DASSLZ010000147 DASSLZ010000000
VERSION     DASSLZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684166"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..713)
                     /locus_tag="VFM00_05530"
     CDS             complement(171..713)
                     /locus_tag="VFM00_05530"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7497740.1"
                     /translation="MSTTLALPDVLDLGCGTAKHVGAFGVDHDPAVSPDLLHDLDRYP
                     LPLPSDHFRVVYCQDVLEHIREIGPFLSEIHRVCRRGARVHVRTPHYSSWYAYNDPTH
                     RHALGCFALDRFTDTPGFHARSTPLFRYVSRRILFPRAYRLIGAAALANAFPARWEQM
                     FAWCFPAENLLFELEVLKEA"
     gene            complement(710..2086)
                     /locus_tag="VFM00_05535"
     CDS             complement(710..2086)
                     /locus_tag="VFM00_05535"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET7497741.1"
                     /translation="MPLTILIAEALLLGLGLLTGHPFLAVAIGAAFVYLLIACRNPHL
                     AWALVWIAFPFSVETLLPGGIAMHVPTEPMILIALAAWGSLVLAGRPFRLPRADLNVP
                     LAALGGAALLSALFGAQPLWGIKALLAISIYVLFGYVFRMVAWDGPSRAERFVPWIVG
                     SAALWGLFGTLRVAALGVSLRNAYGAARPFFVEHGAYAAYLAMILPMALIYALERRGS
                     RRGLYVAASVAIALGIVFSLTRAAWVSLGVVLPITFALWGRWRRSLAPLVPAAVLAVA
                     LALVLPATRSEESLERHVQSITEATDPSNLERLNRWVAAVAMAKDHPILGVGVAAYPD
                     VYRQYRRKIFATDQVYRKMGAHSEPFRLIAEMGVLGLVAALWFLVVAFRTGLRAFFRA
                     PDPATRTLALSIVAGIGTFCLHGLFRTYFDLEKVAVPFWAGLGILAAAGRETAGRESA
                     GSGAESRP"
     gene            complement(2130..>3995)
                     /locus_tag="VFM00_05540"
     CDS             complement(2130..>3995)
                     /locus_tag="VFM00_05540"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HET7497742.1"
                     /translation="LSDDGQTIVFHPDAPFVAGEEVSCSIAPGIRTDIGGILGGATFD
                     FFVAPAPAAPSPAPAALLEDEAPAVNLFVPRDPARAAAMRSDSLPMAGVVVSGSPSEG
                     RVFLCDIRFDASTYRSHLMILGNDGAPLATRDLIGAGYDFKVQPNGKVTCFDADSRHH
                     LVLNAKLAVVDSLQCGNGYTTDVHELQLLPNGHALLLAYDPQTIDMSAVVSGGNAQAR
                     VLGLIVQELDRARDVVFQWRSWDHFQITDCASRPLTGANVDYVHGNSIERDADGNLLI
                     SSRHLDEITKISRSTGAILWRLGGTNNEFTFTNDPERFSQQHSARRLSNGHLLLFDNG
                     NYHTPARSRAVEYTLDEAAKTATKVWEYRNAPDVYATAMGSVQRLPDGHTVIGWGTAS
                     PALTEVTAEGAEVFELTLPAGIYSYRAFRFDWPPTLAAGVAIRSGSVLRAAHGGQVAV
                     LVTGEDFAPDSLDPATVRLQGVAASGYTPPSAPGGAAELLFPVDLLLASQTPGVRQLE
                     LTGSLVSGERIRGAARVTIEGTRTIGARMVSPVGAVPIRIALRSDGVNPRRVRLAAYD
                     VRGRRVCRWTAEADPAGIAAWDGRRPDGAPLASGIYFLRTEGSALTETAKVIIAR"
BASE COUNT          660 a         1364 c         1464 g          507 t
ORIGIN      
        1 caccgagcgg tagttctcca cccagcggtc gggagcgagc acggtgagct cgatttcggg
       61 acgcgccgcg agcgccgcga acttccggcg gtacggcacg acccccgcgg tgtgatcgat
      121 cgcgaggacg cggatcgggc ccgcggcgcc gggcatcgcg cgggcgcggc tcacgcttcc
      181 ttcagcacct ccagctcgaa caggaggttc tcggccggga agcaccaggc gaacatctgc
      241 tcccagcgcg cggggaaggc gttggcgagg gcggccgctc cgatcagccg gtacgcccgg
      301 ggaaagagga tccgccgcga gacgtaccgg aacagcggcg tggagcgcgc gtggaagccg
      361 ggcgtgtcgg tgaaccggtc gagggcgaag cagccgagcg catgccggtg cgtggggtcg
      421 ttgtaggcgt accagctcga gtagtgcggg gtacggacat ggacgcgggc gcccctccgg
      481 cagacgcggt ggatctcgct caggaagggc ccgatctccc ggatgtgctc gagcacgtcc
      541 tggcagtaca cgacgcggaa atgatccgac ggaagcggaa gcgggtagcg gtccagatcg
      601 tgaagcaggt cgggggacac ggcggggtcg tgatccacgc cgaacgcccc gacgtgcttc
      661 gccgtcccgc agccgaggtc cagaacgtcg gggagtgcca gggtggtgct catgggcggc
      721 tctccgcccc ggatcccgcg gattcgcgtc ccgccgtctc gcgcccggcc gcggccagga
      781 tacccaggcc cgcccagaac ggcaccgcga ccttctccag gtcgaagtag gtccggaaga
      841 gcccgtggag gcagaaggtg ccgatgcccg ccacgatcga aagggcgagg gtccgcgttg
      901 cgggatcggg cgcgcggaag aacgcgcgga ggccggtgcg gaacgccacg accaggaacc
      961 agagcgcggc gaccagtccc agaacgccca tctccgcgat caggcggaag ggctcggagt
     1021 gggcccccat cttccggtag acctggtcgg tggcgaagat cttccggcga tactgccggt
     1081 acacgtcggg gtaggccgcc acgcccacgc ccaggatggg gtgatccttc gccatggcca
     1141 cggccgcgac ccagcggttc aggcgctcga ggttcgaggg atcggtcgcc tcggtgatcg
     1201 attgcacgtg ccgctccagc gattcctccg accgcgtcgc gggcaggacc agcgcgagcg
     1261 ccaccgccag gaccgcggcg ggcaccagcg gcgccaggct ccggcgccac cttccccaca
     1321 gggcgaacgt gatcgggagg accacgccca gcgacaccca cgccgcgcgg gtgagcgaga
     1381 agacgatgcc gagcgcgatc gcgacggagg cggccacgta caggccgcgc cggctgcccc
     1441 ggcgctccag cgcgtagatc agcgccatcg gaaggatcat cgcgaggtag gccgcgtagg
     1501 ctccgtgctc gacgaagaag gggcgcgccg cgccgtaggc attacggagc gagacgccga
     1561 gcgcggcgac gcgcagggtg ccgaacagcc cccagagcgc cgccgagccg acgatccagg
     1621 ggacgaagcg ctccgcgcgc gagggaccgt cccaggccac catgcgaaag acgtacccga
     1681 agaggacgta gatcgagatc gccaggagcg ctttgatgcc ccacagaggc tgcgccccga
     1741 agagcgcgga gagcagcgcc gctccgccga gcgccgccag cggcacgttc aggtcggccc
     1801 ggggcagccg gaagggccga ccggccagca ccagcgagcc ccacgccgcc agcgcgatca
     1861 ggatcatcgg ctcggtcggc acgtgcatgg cgatcccacc cggcagaagg gtttccaccg
     1921 agaaggggaa cgcgatccac acgagggccc acgcgaggtg cgggttccgg caggcgatca
     1981 gcaggtagac gaaggcggcc ccgatcgcga cggcgagaaa ggggtgcccg gtcaggagcc
     2041 ccaggcccag caggagggcc tcggcgatca gaatcgtcag cggcacccgg agatcgggcg
     2101 tcagcgggcg gagagcttgg gaccgaggat caccgggcga ttataacctt ggcggtctcg
     2161 gtgagggcgg agccttcggt ccgaaggaag tagatcccgg acgcgagcgg ggcgccgtcc
     2221 ggccgccgcc cgtcccaggc cgcgattccc gcggggtccg cttccgccgt ccagcggcac
     2281 acgcgccggc cccggacgtc gtaggccgcc agccgcaccc gccgtgggtt cacgccgtcg
     2341 gagcgcagcg cgatccggat cggcaccgcg cccaccggcg agaccatgcg cgctccgatc
     2401 gtccgcgtgc cctcgatcgt cacccgggcg gcgcctcgga tccgctctcc gctcacgagc
     2461 gacccggtga gctcgagctg ccggacgccg ggcgtctggg aggcgaggag cagatccacc
     2521 gggaacagga gctcggcggc gccgcccggg gcggaaggcg gcgtgtagcc gctcgccgcc
     2581 acgccctgga gccggaccgt cgcgggatcc agcgaatcgg gcgcgaagtc ctctcccgtg
     2641 accaggacgg cgacctgtcc tccgtgcgcg gcccggagga ccgagcccga tcggatcgcg
     2701 accccggccg caagggtcgg cggccagtcg aaccggaacg cgcgatagga gtagatgccc
     2761 gcggggagcg tcagctcgaa cacctcggct ccctcggccg tgacttcggt cagggccggg
     2821 ctcgcggtcc cccatccgat caccgtatgg ccatcgggca gtctctggac cgagcccatg
     2881 gccgtggcgt aaacgtcggg cgcgttccga tactcccaga ccttcgtcgc ggtcttcgcc
     2941 gcttcgtcca gcgtgtactc caccgcgcgg gagcgggccg gcgtgtggta gttgccgttg
     3001 tcgaagagca gcaggtgccc gttcgacagg cgccgcgccg agtgctgctg cgagaagcgc
     3061 tcgggatcgt tcgtgaaggt gaactcgttg ttcgtgccgc ccagcctcca gagaatcgct
     3121 cccgtcgagc ggctgatctt cgtgatctcg tccagatggc ggctggagat gaggagattc
     3181 ccgtcggcgt cccgctcgat cgagttccca tgcacgtagt ccacgttggc gcccgtgagg
     3241 gggcggctcg cgcagtcggt gatctggaag tggtcccacg accgccactg gaagaccacg
     3301 tccctcgcgc ggtccagctc ctggacgatc agccccagca cgcgcgcctg ggcgttccct
     3361 ccggaaacga cggcgctcat gtcgatggtc tgcgggtcgt aggcgaggag gagcgcgtgg
     3421 ccgttcggga ggagctgtag ctcgtgcacg tccgtcgtgt agccgttccc gcactgcagg
     3481 ctgtccacca ccgcgagctt cgcgttcagg acgagatgat gcctcgaatc cgcgtcgaag
     3541 caggtgacct tcccgttcgg ctggaccttg aagtcgtagc ccgccccgat caggtcgcgc
     3601 gtcgccaacg gcgcgccgtc atttccaagg atcatcaggt gggagcggta ggtcgaggcg
     3661 tcgaaccgga tatcgcagag aaagacccgt ccttccgacg gggatcccga gacgacgact
     3721 ccggccatgg gaagggaatc cgaccgcatg gccgcggccc gcgccgggtc ccgcggaacg
     3781 aagaggttga ccgccggggc ctcatcctcc agcagcgccg ccggagcggg cgagggcgcg
     3841 gcgggcgcgg gcgccacgaa aaagtcgaac gtcgcgccgc ccaggattcc gccgatgtcg
     3901 gtgcggatgc ccggcgcgat ggagcaggag acctcttccc ccgccacgaa cggcgcgtcg
     3961 ggatggaaga cgatcgtctg tccgtcgtcg gagag
//
LOCUS       DASSLZ010000148        31303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1190738, whole genome shotgun sequence.
ACCESSION   DASSLZ010000148 DASSLZ010000000
VERSION     DASSLZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 31303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31303
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190738"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFM00_05545"
     CDS             <1..369
                     /locus_tag="VFM00_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497743.1"
                     /translation="LQRAQRRQDPALENSLRLPSTSTLSVQAERFFRLWGQNVTFYVQ
                     GSNLLDAKNISDIQPSLWPDNQVDANSYQIYYTETGRAGGAFLTADQTGDGVEDWYPV
                     NDPRVYQQGRVIRVGLGVQF"
     gene            383..3181
                     /locus_tag="VFM00_05550"
     CDS             383..3181
                     /locus_tag="VFM00_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497744.1"
                     /translation="MMRTTARGWSGAPSVAAAARRIGALAAGVVLFGGTVAWHTPPEG
                     TGSTGGKGDPQRLFRLDGKFVHNVGRLQLQITNLGETGNQNNPSRTTVPSAEWPAGSG
                     NDYLYAAGLWVGAIDASGIPHVTTALYQREFSPQVDFSSGPAPCANLPLDQIADVRES
                     YEGIPGGNRVISASVDPDDDGDGLVDEDFPNGLDDDGDGLCDEDYAAVGQQMFATEYF
                     DDIPAIRQFLPEHVPLGIRVRQTSYAWATPGQNDFVGMDYQITNRSGRTLRNVMVAIF
                     ADPDIGNRGIQGYFQDDQVGLFDRQVTYVGSTGQTVKRRIQMAYAYDNPDNPNNPQEA
                     KGGDAPGWFGCMFLNHTVDPSGQTAPNHVGVTSFKFFSGSGSFIQGGDPENDAQRYQL
                     MTDPQLNPDVTGQQQSSRPLDYRFIMATGPFRTLLPDSTLTISVGWVMGLGFGLPDGA
                     APPPSILNASDMIGGSLIANAVSAQQVFDGLYTDLDGRLSTGTCGKETCLNSPQSILF
                     QYPPGNVWPDSSCIIRFRPEPSTDPRFANVDYFNPTSDCVTGGGRFWCIDNDLDPNIG
                     HCASGDTTQLRISSPACVYIDQDCDVNTGQGGREHLVHWVAASPLPAPFFSGVDGRDE
                     PRDFLGAYDTKAESTRVSAWFFPGDRKVTLKWNNYGELVRDAQRGNLKEFIGYRIYKA
                     SGWNRPFGTNAPGKNLWSLLGEWRLDPTGTAAHPLSELIDPSVGFTRIDSVRVSWDPI
                     LKRVTTTTRIEVDTLFAVGRYSFVDTHVLNGFPYFYSIVPVSVVPGNAQVADITLVGN
                     PSATNAQVVYPRSDAQNDQKHVYVVPNPYKAQTEWDLVPREEDPSGTKVTFHNLPRTK
                     GTIHIFTLAGDLVRDLPFDATAPPDLQYGKNPVTAGQGEVSWNLISRNGQKIVSGIYL
                     FSVDTDLGREVGKFVIIR"
     gene            3232..4170
                     /locus_tag="VFM00_05555"
     CDS             3232..4170
                     /locus_tag="VFM00_05555"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET7497745.1"
                     /translation="MRSALVLAVLLLLPLSAEGAQIFEKVGTLGGQSLKIGVGARAAA
                     MGEAYVALSDDATAVYWNPAGIARMSGQSISLNHTSWPADILFDQAAYVFNIKWIPGM
                     LGVNVRALTMSRDIVRTTYLPEGTGDTFDAGEWAYGLSYARALTDKFSAGFSVNYVQT
                     GLDDVKGSSTTFDFGTLYDVGVLGAKIGMSIQNIGSDMTFIDEKVKMPVFFRVGGSFD
                     VMQQGENRLIAAAEFTHPPDNSEKLNLGAEYGFHDYLFLRGGYKLNYDTQGLTAGFGV
                     KFPLTLVKSSVARLDYAYQDMNFLGTTHRVSLNIGF"
     gene            4193..5695
                     /locus_tag="VFM00_05560"
     CDS             4193..5695
                     /locus_tag="VFM00_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HET7497746.1"
                     /translation="MRRSRTGQAIVAAAALLVLASGSPAAPPPPGSVAEGEAAASAGE
                     IHFHARAIPYRHSPSEGRAEFSIRVPYREIKFIPKTDSLFEGLLRVTVEMWDAAEKRI
                     GYRQQEARVQVTDLSAAGDSLLGEVYKLGLTAKPGRYAYRVTVEDMNMARMGLVYKMK
                     NQKRQGKVEGLVDLGPWLFRNPSLSGIEPAWEISAAKEGAPFRKGPYDVYPHPSGYYG
                     LYKDEVSGYYEIYDDPPPPEGRSYRVRSLLVSSKGDTIPTGEDSLRVTEGTAWPHAIS
                     IDATGLAAGHYRLALEIRREGQPVSAGTSSEFDILWSRDSWRPDAAEFYDVAAGVLLS
                     SEDAAKFPRLSMGEKEVRIEEAWQSADPSPETAENEARIEFLRRVAYANQHYTIFTPG
                     MFSDRGRVYIRYGEPDEMKIERLPVAGKTIGHVLDSSIPQASRETITRTDTGIADSRP
                     YEIWTYNSHGHEASARHGLNEVASGLKFVFIDDQGYGEYTLRYSSTTGIH"
     gene            5829..6866
                     /locus_tag="VFM00_05565"
     CDS             5829..6866
                     /locus_tag="VFM00_05565"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET7497747.1"
                     /translation="MRIGMLTGGGDCPGLNAVIRAVVQRAVKTYDWDVCGIRHGWRGL
                     LVDEIDALDLAKVSGILPRGGTILGTSRTNPFKEADGPARIRAALATHAIDALVAIGG
                     EDTLGTANKLAQDGLPIVGVPKTIDNDVNGTDLTFGFDTALTIATEAIDRLHTTAESH
                     DRVMVVEVMGRHAGWIALESGIAGGADLILIPEFPVSFSEVTEVVRRRHDRGKSFSII
                     VVAEGAHLADDGGQALHLASEKMDAFGHVRLGGIGATLAERLEEQTGFETRYTILGHI
                     QRGGSPTAFDRVLGSRFGIAAVDLIAKQGYGRMVALRGTQIVDVPLAEAVGTLKTVGS
                     ELYELARVFSS"
     gene            6876..7877
                     /gene="gap"
                     /locus_tag="VFM00_05570"
     CDS             6876..7877
                     /gene="gap"
                     /locus_tag="VFM00_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7497748.1"
                     /translation="MRVAINGFGRIGRLVLRAALKRKETIDFVAVNDITDAKTLAHLL
                     TYDSIHGPWPERFSAAEGGLRVGTTTIRVLCEAKPENLPWKDLGVDVVLECTGRFTDR
                     DKAAVHLGAGAKRVLVSAPSKGADATFVMGVNDKTYDPAKHQVVSIASCTTNCLAPVL
                     SVLDESFGIERGFMTTIHAYTNDQKVLDAPHKDLRRARSAAQSMIPTSTGAAKAIGLV
                     IPSLQGKLDGIAVRVPIPCGSIIDVAAETARPVSAAAVNDAMRAASTGRLSGILQYME
                     DPIVSADIVGNPHSAIFDAPLTMAQGDRLVKVFAWYDNEWGFSNRMVDALLLLGKAS"
     gene            7888..9093
                     /locus_tag="VFM00_05575"
     CDS             7888..9093
                     /locus_tag="VFM00_05575"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7497749.1"
                     /translation="MTFRRLNHLEAEGRTIFVRVDFNCPLKPEGGVADDTRIVASLPT
                     LRYLLDKGARLVVASHLGRPKGKRDAKQSLMPVREVLEKYLRGPVRFAPSIIGPEAEA
                     EARALEPGQALLLENLRFDPREEANDPEFSRALAALAGAYVNDAFGSAHRAHASTVGI
                     TAYLADRAAGFLMEKELSALGRLLEGAARPYVAILGGAKISGKIDVLQNLLPRVDRVF
                     VGGGMMFTFLRAKGLETGRSLVEEDRVPLAAELLRSAEGSKLLLPEDCRAAADVKGED
                     PGRIVPVTAIPSDRMGVDIGPASLGAVGSALAEAKTVFWNGPMGIFEVPTYAEGTIGV
                     AREMARATKRGAFTVVGGGDSLAAVNAAEVEHQISHLSTGGGASLEFLEGKELPGVAA
                     LEDAPRPAR"
     gene            9090..9854
                     /gene="tpiA"
                     /locus_tag="VFM00_05580"
     CDS             9090..9854
                     /gene="tpiA"
                     /locus_tag="VFM00_05580"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET7497750.1"
                     /translation="MKRILAGNWKMNLLLAEARALYEEVAAGCRERSGIACVLFPPAT
                     ALAALAAARTEEGPALGVQNCHFESKGAFTGEVSAEQARDAGARYALVGHSERRRLFC
                     ESDQLVRQKLIAAWRTGLHPVLCIGETLAERERRETRDVLARQLRRALEGSPGKAPLW
                     VAYEPVWAIGTGVVASVEEVVEAHAWVREELAAAGRGAAGDPVPILYGGSVDPKNAGP
                     LAAAPGVDGFLVGGASLQAASFLEIGRLLEQTVPAH"
     gene            9928..10482
                     /gene="secG"
                     /locus_tag="VFM00_05585"
     CDS             9928..10482
                     /gene="secG"
                     /locus_tag="VFM00_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET7497751.1"
                     /translation="MSGVILGLVVTIHVLACIALMITILLQSGKGGGLAGAFGGGSSQ
                     TLFGGRGAAGFLSRATTVLAILFFVTSLTLGLSSSKATSRRSLVQEEARRRAAGAPPV
                     GSGATAPSPGASSPLGGGTVTPAPQGGGMAPAPPSGTAPAPRGTTAPSPSGTTAPPSG
                     PATSGGATTPPASGSGQSPATGGQ"
     gene            10493..10576
                     /locus_tag="VFM00_05590"
     tRNA            10493..10576
                     /locus_tag="VFM00_05590"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10528..10530,aa:Leu,seq:gag)
     gene            complement(10676..10933)
                     /locus_tag="VFM00_05595"
     CDS             complement(10676..10933)
                     /locus_tag="VFM00_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497752.1"
                     /translation="MPEMSEIVSSPAVECLRCSRLFGPNQIFHLKEDPRRAYIRCQHC
                     GGRNEIRAEERFCMRLVGILPEPAASRAGAQAMSTPENPVP"
     gene            11050..11877
                     /locus_tag="VFM00_05600"
     CDS             11050..11877
                     /locus_tag="VFM00_05600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7497753.1"
                     /translation="MTGRAPETGYDAARAAAYWGGARHETGDELAAVLSLGEPPHVNA
                     AYDAWETGLLMDVIGSADRGLDLGAGVGRVSIRLSSRVARLACGDLAPGMLERLRRNA
                     ARAGAMGLDPVRLRSDRLPFREGSFPVAVCLGLLEHLPPEARRATLAELARVVRPGGL
                     LVLVLNNERSALLRDPSDNPHRVGAQRENGYYCAVVPEAALLDEAKPHFRDEALGSNL
                     FYALQRHASRALDERRRRDARLAPFFERAAAWDRALRPRGPMADAAADHHLHLLVRR"
     gene            11874..12845
                     /locus_tag="VFM00_05605"
     CDS             11874..12845
                     /locus_tag="VFM00_05605"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7497754.1"
                     /translation="MSRPPILVTGGAGYIGSVLVRRLLEDGARVRVLDRFLFGGQGLD
                     GIASDGSLTVLHRDLRDPRAHAEALEGVETVIHLAAIVGDKACAQDEERAVQTNWTAT
                     VAFARSAAARGIRRFVFASTCSVYGEGREETLTEESPVKPLSLYAETRWHAEQGLLEL
                     ASEGTLQPVLLRFGTVYGLSPRMRFDLVVNLLARDAVLKGAVTIFGGDQWRPFVHVSD
                     IARALRLAAFEPLPEDSPIVNVGDNLENYQLKDLKEAIEENVPGASVTIQPAAKDPRT
                     YRVSFDKIERLWGFRAERRVGEGIAEIAAAVRGGRIPQPYDARYVNA"
     gene            12945..13880
                     /locus_tag="VFM00_05610"
     CDS             12945..13880
                     /locus_tag="VFM00_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7497755.1"
                     /translation="MPRLEFDPTQLRERALFVRRDIITLLARSQSGHSGGPLSSADFG
                     SVLFFREMNLDPARLDDPGRDLWHFSIGHITPCIYALMGERGYFPLRDLLQFRRFEGH
                     LQGHPSKHDTPGVEVSSGSLGQGLSVCCGMALGSRMDKNPRRVYCVMGDGEQQEGQIW
                     EAAMFAAHYKLDNLCGVIDYNRKQIDGDVQDVMGIAPLADKWRAFNWNVIICDGHDIP
                     SIIDAFDGARATKGKPSVILAKTVMGKGVSYMEDDYRWHGVPPSVEQAEKALAELGTT
                     LEAWTKRLESHGAPQVAPSAAPATPVPAAVAGGAR"
     gene            13877..14881
                     /locus_tag="VFM00_05615"
     CDS             13877..14881
                     /locus_tag="VFM00_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET7497756.1"
                     /translation="MSSGTAPAAPRHAEPATLAKPQMKRTREGFGHGLVDLGEKDPRI
                     VVLVGDLTGSTNVNFFAEKFPDRFIQVGIAEQNMMCVAAGLAAVGKIPFLATYGAFAS
                     CRSADQIRVTVAYTNLPVKIGGAHGGISVGPDGATHQAMEEIAIIRSIPNMRMIVPAD
                     YDETRKATVAAGSDLHPYYIRFGREPVPVVTDAGSPFVIGRANQLREGKDLAIIACGV
                     MVYEALVAAEALAARGIECRVLNMHTLKPLDKEAIERAARDCGAIVTAEEHQVHGGLG
                     GAVAEAVVAAHPVPMELVAVHDRFGRSGKQDELMAAFGIKAPDVIRAAERVLARKRGR
                     "
     gene            14910..16115
                     /locus_tag="VFM00_05620"
     CDS             14910..16115
                     /locus_tag="VFM00_05620"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET7497757.1"
                     /translation="MRVPLRAAATATALIAALASVAPFTHAQTPREAPPPTSLSSTDT
                     LAVPVRVVLGQGPAFAVAAFDSATGSWGAACAASEIAVGARALEASAETGALLSLGTG
                     GGDLGAAATALARGMDPDTALGLLVPSGTDPAGRLGIVVARGGRVAARSGAHLPGFSG
                     TVLRPRFACAGYGLRGAASLEAMDEAFAQRGGDLGARLLAALEAGERADGDPFAARGK
                     EASAALLVVRAVTESGSGTGTDRVTDLRVDLDKDPIGALGRIYARHAETFLPAAHVRY
                     GDAARRRGDDAAAAREYAAAEAGFRAAVARGPKDADALNELAWFLATRGGDPVEALRY
                     AEAAVGTRSDDPNLLDTLAEAAYRAGNLERAIEAAERAVRLSRGNERYAERLRAFRAA
                     RAALTPPSR"
     gene            complement(16105..16896)
                     /gene="bshB1"
                     /locus_tag="VFM00_05625"
     CDS             complement(16105..16896)
                     /gene="bshB1"
                     /locus_tag="VFM00_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HET7497758.1"
                     /translation="MAPARDLDLPSSHDGPPLDLLAIGAHPDDVEISCGGTIAAAAAE
                     GLRAGILDLTRGESATNGTPEIRAAEAAEAARLLHACGRWNAGLPDGGLHAHDPEQVR
                     RAVRWLRALRPDVVVVHFPKDRHPDHVAASELIDRASYLAGLRRFEGGGDAAPFRPRV
                     RYHFASRVGFAPSFVVDVTAHWEAKRAAILAHGSQVSRASADARPTPLNREGFLDFVE
                     SRARHYGGMIGVTYGEPFHATEPIGVRALAPFLRTPQPAPGSFTG"
     gene            complement(16901..17896)
                     /gene="glpX"
                     /locus_tag="VFM00_05630"
     CDS             complement(16901..17896)
                     /gene="glpX"
                     /locus_tag="VFM00_05630"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HET7497759.1"
                     /translation="MDRHVGLDLVRVTEAAALACARFMGRGDAHGADQAAVTAMRRAF
                     DNVDIRGTVVIGEGERDEAPMLYIGERVGSGYGPEVDIALDPLECTNSVAFGRDNALA
                     VIALAQKGHFLHAPDTYMEKIAVGAKAANAIDLSRSVEENLESIAAAKGYDLEDLTVV
                     VLDRPRHEELIQRIRKVGARIHLIPDGDVSAAMATAVESTGVDVLVGIGGAPEGVLAA
                     AALRCMGGAMQGRLKFRSDEEKQRAKRMGITDHDRIYSETDLARGDSIIFATTGVTDG
                     DMLDGVRFSGDGAETHSMVMRAATGTVRILRTSHRFSGPDGRPVSIEPFEAFHRS"
     gene            complement(17896..18234)
                     /locus_tag="VFM00_05635"
     CDS             complement(17896..18234)
                     /locus_tag="VFM00_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497760.1"
                     /translation="MNDQRWEEVLRRLDKHFGNLEFDESEDEETHAVSESVTWESPQG
                     RMKLVRTTKPLVVDKKMHYSNRAGGGTHVEYVYSKTESTSRIRLFRWIDARNDWEEID
                     ATSLTGPEAR"
     gene            18287..19087
                     /locus_tag="VFM00_05640"
     CDS             18287..19087
                     /locus_tag="VFM00_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7497761.1"
                     /translation="MAPTEDAPLASGFQGRSALVVGGSRGLGRAVAIALAREGCRVLA
                     VGRSAAPLAALRETAAARGLRLRTARGDVTRPGTARRLVRQAGALSEGGGRGRLGHAA
                     PDIVVHAAGDYWEGPLRALTPERWEALVHSNVTGAVALLHAALPPMRRRRFGRVLLFG
                     VAGGDMPRAASRAHGYRAAKIALLTIARSAAQEEAEHGITINVILPGVIRTPGTPARW
                     AAAAAAAKIPARRLGTPGEVARAALFLLAEESGYITGAALPVSGGYLL"
     gene            19214..20932
                     /gene="recJ"
                     /locus_tag="VFM00_05645"
     CDS             19214..20932
                     /gene="recJ"
                     /locus_tag="VFM00_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET7497762.1"
                     /translation="MRPSTPKTWVLKSPAAPELAERLKAELGVSDTFARLLANRGFTS
                     SEQVESFLVPSTDKLLDAFTMRDMDRAVDRVLQAVAARESILVYGDYDVDGITSTSLL
                     TAALAKMGAKVSYFIPDRIRDGYGFSERGVDVARKRRIRLVITADCGITATNEVRLAQ
                     KEGIDVIVTDHHEPLGELPQASAVLNPKRKDCPYPFKELAGVGVVFKLVQGLAARRPD
                     VLPPDFVFHHLDLVALGTIADVVPLVGENRVFAKIGLERLCHSDKPGIAALKEVAGLR
                     TRRVESGHVAYILAPRINAAGRLGNAESGVRLLLSNDPREAAIIAESLEEDNANRKKI
                     DESTLEDALGQLQKHGSELPPAIVLWSDRWHPGVLGIVASRLIERFHRPTILIASDGD
                     EGKGSGRSIPGFDVCLALQECRAHLLGFGGHSYAAGLTIRTECLEAFRDHLCDVVRTK
                     VQPDDFIPKLSIDGPIDLEHLNEDLVQALDRLAPFGIGNAEPLFVADDVKLSLPPAVV
                     SRNHLKLTIRQNGRDLDCIGFGMGHMASVIAQNGGKLSVAFVPTINVWQNRSRLQLKL
                     RDIQVR"
     gene            20935..23139
                     /locus_tag="VFM00_05650"
     CDS             20935..23139
                     /locus_tag="VFM00_05650"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET7497763.1"
                     /translation="MVPAPPDALGPLLEALHKTGKPFHLEKVREAYEFAARAHANQKR
                     RSGEPFVTHSVAVAAILVDLLGTGLDDTLLEAALLHDVVEDTEIDLPAIQRAFGPEVA
                     ALVDGVTKISGLHFDRPETEQAENFRKMLLSMARDLRVILIKLADRLHNMRTLNALNP
                     ERARAIARETREIYAPLAHRLGIARIKWELDDLCLKHLDFEAFQELRQKVALKREERE
                     RQIQEVMGPVKDRLVAAGIKADVMGRPKHLESIYRKMRTQERAFEEIFDLLGIRVITR
                     DKAECYRVLGVVHDLFTPVHERFKDYIATPKSNMYQSLHTTVVAPGGLMVEFQIRTAE
                     MHQIAELGVAAHYRYKEGGRQDAELEAKLAPLLRGADFQSATTGDPDEFMEYLKTSLY
                     QDEVFVFTPRGDLRRLPRGATPLDFAYLVHTQVGNHCVGAKVNGRIVPLRTELRSGDT
                     VQIITSPGAKPNRDWLKLVRTHAARSKIRHWLKVAEHSDSVALGKEIIERELRRRRLT
                     PPAGVSLESLAQSMGHPDVNGLYAALGQGNLSVQQVIGKWIPDGGGPVQRLKEASLET
                     IRAITGRPGHGVRIQGVDNLMVAYAKCCQPVPGDPVVGIVTRGRGVTVHRTDCPNTFD
                     DRVPAERRMVVDWSAKPEDSFTVKLSIFGQDRKSLLADIAKAISTTNTNIRTAGIKAN
                     DRNARGAFVVEVRDLAHLREVMRAIENVDGVDAVEREQVFGKPGHGEAGLAS"
     gene            23136..23579
                     /gene="dtd"
                     /locus_tag="VFM00_05655"
     CDS             23136..23579
                     /gene="dtd"
                     /locus_tag="VFM00_05655"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET7497764.1"
                     /translation="MRALLQRVSRAAVRVEDEEIARIGPGLLILLGVGASDPDAAAPR
                     LAERCALLRIFEDEAGRMNRSVIDVAGEALVVSQFTLVADVSRGRRPGFDPAAPPAAA
                     EAAYERFCAALEGAGVPVRRGRFGAPMQVELVNEGPATFLLETPS"
     gene            23602..24267
                     /locus_tag="VFM00_05660"
     CDS             23602..24267
                     /locus_tag="VFM00_05660"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017941114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET7497765.1"
                     /translation="MRPNLLNPLGPKLRLASRSPRRAELLTLVGAHFEIAPVELDESA
                     LPGERPDAHVLRLAEAKARASRAARDAAGAGQDAGAGPAGDGPEGTVYVGADTVVVID
                     RVILGKPADRADAARMLGLLSGRVHEVWTGLFILDPAEERGLGEAVRSIVKFSPIGAA
                     MIDRYVATGEPLDKAGAYAVQGGGALFVEAIEGSYSNVVGLPLSHLKHLLGLLREAPV
                     REP"
     gene            24277..25326
                     /gene="mtnA"
                     /locus_tag="VFM00_05665"
     CDS             24277..25326
                     /gene="mtnA"
                     /locus_tag="VFM00_05665"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET7497766.1"
                     /translation="MDPLRIEKGALEVLDQTLLPERESWIAVDGAAAMAEAIARLAVR
                     GAPAIGIAAALALAAEARRRAAASSDAAPLLAALRASGALLRATRPTAVNLAWAVDRT
                     LAACASAAAEGAGAGAIAERAQREAEAIWEEDRAASRAMAAHGATLLGSRRRFLTHCN
                     TGGLATGGGGTALGVILEMHRRHPSAVEVWHTETRPLLQGARLTAWELVRAGVVPRLI
                     ADGAAAHVIAREGIEAVLVGADRIARNGDAANKIGTYGLALAAHASGIPFVVVAPTST
                     MDPSLEDGASIPIEERDSSELTLLRGIPTAAAGVLGRNPAFDVTPARFISWVVTERGA
                     ARPGETPAGMVDRVV"
     gene            25384..25836
                     /gene="rplM"
                     /locus_tag="VFM00_05670"
     CDS             25384..25836
                     /gene="rplM"
                     /locus_tag="VFM00_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET7497767.1"
                     /translation="MGTYSVRASEIQQDWYVVDAEGQILGRLASEIASVLKGKRKPTY
                     STHLDVGDNVIVVNAEKVLVTGRKRDNKIYFRHSLYPGGDTRTRFRDLQAQKPDEIIR
                     LAVKGMMPKNRLGRAMMKKLKVYAGSEHPHAAQMPRPFPMPGATTGGA"
     gene            25836..26225
                     /gene="rpsI"
                     /locus_tag="VFM00_05675"
     CDS             25836..26225
                     /gene="rpsI"
                     /locus_tag="VFM00_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET7497768.1"
                     /translation="MANPVMATGRRKTSVARVRLVPGNGKRTVNGVEFDRYFCRMVLT
                     RMATEPLLATNLADKYDVIALVRGGGPAGQAGAVSLGVARALQVIDPALRGLLGKEGF
                     LTRDARMKERKKYGQAGARKRFQFSKR"
     gene            26352..27113
                     /gene="rpsB"
                     /locus_tag="VFM00_05680"
     CDS             26352..27113
                     /gene="rpsB"
                     /locus_tag="VFM00_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010649266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET7497769.1"
                     /translation="MADIGMKELLEAGVHFGHQTRRWNPKMKKFIFIERNGIYIIDLQ
                     KTLKAIQDARALLEGVARRSGTVLFVGTKRQAKDAVREEAERSGMPYVNERWLGGMLT
                     NFKTIKSNIRRFKEIEKMDAEGVLEKLSKKEQAQINKERVRLVKIFTGIKEMGQLPAA
                     VFVIDTKKERIAVAEANRLGIPVIGVVDTNCDPDVIDFPLPGNDDAIRAIRLFSRFVS
                     DTLMSTRQEMQEGADREAMAAQAGAAPETPAVASA"
     gene            27174..27770
                     /gene="tsf"
                     /locus_tag="VFM00_05685"
     CDS             27174..27770
                     /gene="tsf"
                     /locus_tag="VFM00_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET7497770.1"
                     /translation="MTITASHVKDLRERTGAGMMECKKALAEANGDIEKAIEVLRKSG
                     MARASAKAGREARQGTVHAYIHPGDQLGVLIEVNCETDFVARTDDFKHLVRSLAMQVA
                     ATNAQAVDRAALDPAFIAGERAILMEQAKATGKPEAVIQKIVDGKIEKMYEEVVLLEQ
                     PTIRDPDRKVKELLDQAIAKLGENIVVRRFAKFKIGEA"
     gene            27779..28510
                     /gene="pyrH"
                     /locus_tag="VFM00_05690"
     CDS             27779..28510
                     /gene="pyrH"
                     /locus_tag="VFM00_05690"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET7497771.1"
                     /translation="MAPSSEVPAPHRVLLKLSGEILAGEARFGIDAEALGRVADEIVE
                     GARAGTQVGIVIGGGNMIRGTATGGALDRTTADSMGMLGTVINALALQDALEERDLST
                     RVLSAIEMRSLAEPYIRRRAVRHLEKGRVVILAAGTGHPYFTTDTAAVLRAVEIRADV
                     LLKATKVDGVFTDDPMRNPDAVRFDTLSYLEAIDRRLNVMDMTAFTMGMDNQLPILVF
                     NVTVPGNLSRALRGEIVGTRVAVEV"
     gene            28513..29070
                     /gene="frr"
                     /locus_tag="VFM00_05695"
     CDS             28513..29070
                     /gene="frr"
                     /locus_tag="VFM00_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET7497772.1"
                     /translation="MGPEILKQTEERMKKAVETIRHEVATVRTNRATTALLDGIKVEY
                     YGNPTPLQQVASVSVPEPRMLLIQPWERAILPEIVKAIQKSDLGLNPADDGSVVRLTL
                     PTLTEERRKDLVKHLGKLIEDGRVRVRTVRREVNDELKKLEREHKISEDDAKKTHDAV
                     QKIHDRYIVMLDDLFHKKQAEVMEV"
     gene            29094..29840
                     /locus_tag="VFM00_05700"
     CDS             29094..29840
                     /locus_tag="VFM00_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET7497773.1"
                     /translation="MRAPSKAPTLEQVLRRPVPEHIAIIMDGNGRWAKRRGLPRLMGH
                     RAGRRSVREAVEGCVELGVRVLTLYTFSLENWERRAGEVRGLWRILEQVLREERNELK
                     KNNVRLRTIGRVDLLPERSRAILAETMQFLESSTGLTLVLAISYGGRAEIVDAARRIV
                     AADRKQRIPLTKIDESYFQSQLDTDGLPDPDLLIRTSGELRVSNFLLWQLAYAEFWVT
                     DTLWPDFRRKHLYEAVHDFQNRSRRFGRAD"
     gene            29797..30750
                     /locus_tag="VFM00_05705"
     CDS             29797..30750
                     /locus_tag="VFM00_05705"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET7497774.1"
                     /translation="MTFKTEAGASAARIDPAARDGVTRDAEGAAARPRGDAALPLRIA
                     SAVVFLPLFVLAARVGGLVWLAFTLIVVGLGLREFYRMMDSKGLAPHWKSGTFAGLLL
                     PIGTYLRLQTGRTAEWNLGALFTVLAGAILLAELRRGAGKQAVGSIAATLLGVIYLGW
                     FGSHIELLRELPRAWHLPYALGASFAILPFFLVWICDTAAYAVGLAFGRTRLMPDVSP
                     QKSVEGAVAGLLAAVGAAFAARAWFAPYLTALDAAVLGTLVGVLGQLGDLVESLLKRD
                     ADAKDTSRIIPGHGGVLDRFDSVLFAAPVTAYYLLFRVLDR"
     gene            30747..>31303
                     /locus_tag="VFM00_05710"
     CDS             30747..>31303
                     /locus_tag="VFM00_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET7497775.1"
                     /translation="MIRIALLGATGSIGAAAQALARAHPDRMRLVSLSARANVESVLR
                     AAEEFGVSRVALSDPEAARALRARFRGEVLEGDLAATRLAEDPDADVVVNALVGSAGL
                     SPTVAALHAGKRVALANKESVVLAGELLAKVAAQGGGTIVPVDSEHGGIHQALEGCAL
                     DTIRRVTLTASGGPFLRRDPATLADV"
BASE COUNT         4806 a        10869 c        11042 g         4586 t
ORIGIN      
        1 ttgcagcggg cgcagcggcg gcaggatccc gcgctcgaga actcgttgcg gcttccctcc
       61 acctcgaccc tttcggtcca ggcggagcgg ttcttccggc tctgggggca gaacgtcacg
      121 ttctacgtcc agggatcgaa cctcctggac gcgaagaaca tctccgacat ccagccgagc
      181 ctctggccgg acaatcaggt cgatgccaat tcctatcaga tctactacac ggaaacgggc
      241 cgtgccggcg gcgccttctt gacggcggac cagacagggg acggggtcga ggactggtac
      301 cccgtgaacg acccgcgcgt gtaccagcag ggtcgcgtga tccgcgtcgg attgggggtc
      361 cagttctgac atgaagaagg cgatgatgcg aaccacggca agaggatggt ccggggcgcc
      421 ctcggtcgcc gcggcggctc gcaggatcgg cgcgctcgcg gcaggcgtcg tcctgttcgg
      481 cggaacggtg gcgtggcaca cgccgcccga gggcaccgga agcacgggcg gcaaggggga
      541 tcctcagcgc ctgttccggc tggacggcaa gttcgtccac aacgtgggac ggctccagct
      601 ccagatcacc aacctcggcg agaccggaaa ccagaacaac ccctcgcgca ccacggtgcc
      661 ctccgcggag tggccggcgg gctcggggaa cgactatctc tacgcggccg gcctctgggt
      721 cggggcgatc gacgcgagcg gcatcccgca cgtgaccacg gcgctctacc agcgggagtt
      781 ctcgccgcag gtggatttca gctcgggccc cgctccgtgc gccaacctgc cgctcgacca
      841 gatcgccgac gtgcgggaat cctacgaggg gatccccggc ggcaaccggg tcatctccgc
      901 gagcgtcgac cccgatgacg acggcgacgg cctggtggac gaggacttcc cgaatgggct
      961 ggacgacgac ggggatggcc tctgcgacga ggattacgcg gccgtcggac agcagatgtt
     1021 cgcgaccgag tacttcgacg acatccccgc catccggcag ttccttccgg aacacgtgcc
     1081 gctcggcatc cgcgtgcgcc agacctccta tgcctgggcg acacccgggc agaacgactt
     1141 cgtgggcatg gactaccaga tcacgaaccg gagcggccgc acgctccgga acgtgatggt
     1201 ggcgatcttc gccgacccgg acatcggcaa tcgaggcatc cagggctact tccaggacga
     1261 ccaggtgggt ctcttcgacc ggcaggtcac ctatgtcggc tccaccggcc agaccgtgaa
     1321 gcggcggatc cagatggcct acgcttacga caacccggac aatccgaaca atcctcagga
     1381 ggccaagggc ggggacgcgc cgggctggtt cggctgcatg ttcctgaacc acacggtgga
     1441 tccgtcgggc cagaccgccc cgaaccacgt gggtgtgacg tcattcaagt tcttctcggg
     1501 ctccggctcc ttcatccagg gcggcgaccc ggagaacgac gcgcagcgct atcagctcat
     1561 gaccgacccg cagctgaacc cggacgtgac ggggcagcag cagtccagcc gtccgctcga
     1621 ctaccgcttc atcatggcca ccggcccgtt ccggacgctg ctccccgatt ccaccctgac
     1681 gatctcggtg gggtgggtga tggggcttgg attcgggctt ccggatggcg cggcgcctcc
     1741 tccgtccatc ctgaacgcct cggatatgat tggcgggagt ctcatcgcga acgcggtctc
     1801 ggcccagcag gtgttcgacg ggctctacac ggaccttgac ggccggctct cgaccggcac
     1861 ctgcggcaag gagacctgtc tcaattcgcc ccagtcgatc ctcttccagt atccgccggg
     1921 gaacgtctgg ccggactcgt cctgcatcat ccggttccgg ccggagcctt cgaccgatcc
     1981 ccggttcgcg aacgtcgact acttcaatcc caccagcgac tgcgttaccg gaggcggtcg
     2041 cttctggtgt atcgacaacg acctcgaccc caacatcggc cattgcgcgt cgggagacac
     2101 gacgcagctc cgcatcagca gcccggcctg cgtctacatc gaccaggatt gcgacgtgaa
     2161 caccgggcag ggcggaaggg agcacctggt ccattgggtg gccgcatcgc ccttgcccgc
     2221 gcccttcttc tccggcgtgg acgggcgcga cgagccgcgc gacttcctgg gcgcctacga
     2281 caccaaggcc gagtcgacgc gcgtctccgc gtggttcttc ccgggggacc ggaaggtcac
     2341 gctcaagtgg aacaactacg gcgagctggt ccgggacgcc cagcgaggca atctcaagga
     2401 gttcatcggg taccggatct acaaggcgtc cgggtggaat cgtccgttcg ggacgaacgc
     2461 cccggggaag aacctatggt cgctcctcgg tgaatggcgc ctcgatccca cgggaacggc
     2521 cgcgcatccg ctttcggagc tgatcgatcc gtcggtcggg tttacccgga tcgattcggt
     2581 gcgcgtctcc tgggacccga tcttgaaacg ggtcaccacg acgacccgca tcgaggtgga
     2641 caccctgttc gccgtgggcc gctactcctt cgtggacacc cacgtcctga acggattccc
     2701 gtacttctac tccatcgttc cggtctcggt cgtgcccggg aacgcgcagg tggcggatat
     2761 caccctggtc gggaatccga gcgccacgaa cgcgcaggtg gtctatccga ggagtgacgc
     2821 ccagaacgac cagaagcacg tgtacgtcgt gcccaatccc tacaaggcac agacggaatg
     2881 ggatctcgtg ccccgcgagg aggatccctc cgggaccaag gtcacgttcc acaatctgcc
     2941 gcgcaccaag gggacgattc acatcttcac cctggcgggg gatctggtgc gcgaccttcc
     3001 gttcgacgcg acggctccgc ccgatctgca gtacggcaag aacccggtga ccgcggggca
     3061 gggtgaggtg tcgtggaacc tgatctcgcg caacggtcag aagatcgtga gcggaatcta
     3121 tctcttctcc gtggacaccg acctcggcag ggaggtcggg aagttcgtga tcatccgatg
     3181 aacgccgccg gaacgaccca tcctaccgtg ggcggaccga ggaggaactt cgtgagaagc
     3241 gcgctcgtat tggccgttct actgctgctc ccgctctcgg cggaaggggc ccagatcttc
     3301 gagaaggtcg gcaccctggg aggccagtcg ctgaagatcg gcgtgggggc tcgtgcggcg
     3361 gccatgggag aggcctacgt cgcgctctcc gacgatgcca cggccgtcta ctggaacccc
     3421 gcggggatcg cccgcatgtc agggcagtcg atctcgctga accacacgtc gtggccggcc
     3481 gacatcctgt tcgaccaagc cgcgtacgtc ttcaacatca agtggattcc cggcatgctc
     3541 ggcgtgaacg tccgggccct caccatgagc cgggacatcg tccggacgac ctatctgccg
     3601 gaaggcaccg gggatacgtt cgacgccggg gaatgggcgt acgggctctc ctacgcgcgg
     3661 gctctgaccg acaagttctc ggcggggttc agcgtgaact acgtccagac cggcctcgac
     3721 gacgtgaagg gctcgtccac cacgttcgat ttcggcacgc tctacgacgt gggcgtgctg
     3781 ggcgcgaaga tcggcatgtc gatccagaac atcgggagcg acatgacctt catcgacgag
     3841 aaggtcaaga tgccggtgtt cttccgcgtc ggcggctcgt tcgacgtgat gcagcagggc
     3901 gagaaccggc tgatcgctgc cgccgagttc acccatccgc ccgacaactc ggagaagctg
     3961 aacctggggg ccgaatacgg cttccatgac tatctgttcc tccggggcgg gtacaagctc
     4021 aactacgaca cgcagggact gaccgccgga ttcggcgtca agttcccgtt gaccctggtg
     4081 aagtcctcgg tcgcgcgcct ggactatgcc taccaggaca tgaacttcct ggggacgacg
     4141 caccgcgttt cgttgaacat cggattctag ccacgcgaaa ggagtccgcg ccgtgaggcg
     4201 ctcccgaacc ggccaggcca tcgtcgccgc cgctgcactt ctcgtcctgg cctcgggctc
     4261 tcccgcggcg cctcctcccc ccggctcagt ggccgagggg gaggcggccg cgtcggcggg
     4321 cgagatccat ttccacgccc gcgccattcc ttaccgccac agcccctccg aaggccgcgc
     4381 cgaattctcc atccgcgtcc cgtaccgcga gatcaagttc atcccgaaga ccgacagctt
     4441 gttcgaggga ttgcttcgcg tgacggtgga gatgtgggac gcggccgaga agcggatcgg
     4501 ataccgccag caggaagcgc gggtccaggt caccgacctc tcggccgccg gcgactcgct
     4561 gctgggcgag gtctacaagc tgggactcac cgccaagccg gggcgctacg cctaccgggt
     4621 gacggtcgag gacatgaaca tggcgcggat ggggctcgtc tacaaaatga agaatcagaa
     4681 gcggcagggc aaggtggagg gcctggtgga cctgggtccc tggctcttcc gcaatccctc
     4741 gttgagcggc atcgagccag catgggagat ctccgcggcg aaagaggggg ccccgttccg
     4801 gaagggaccc tacgacgtct acccccaccc cagcggctac tacggcctct acaaggacga
     4861 ggtctcgggc tactacgaga tctacgacga cccgccgccg cccgaagggc gcagctatcg
     4921 ggtccgctcg ctcctcgtga gctccaaggg ggacaccatt ccgaccggag aggactctct
     4981 tcgcgtgacg gaggggaccg cctggccgca cgcgatctcg atcgacgcga cgggcctggc
     5041 ggcgggccac taccgcctgg cgctcgaaat ccgaagggaa gggcagcccg tgtcggcggg
     5101 cacctcgtcc gagttcgaca tcctctggtc ccgcgattcc tggcggcccg atgccgccga
     5161 gttctacgac gtcgcggccg gcgtgctgct ctcctcggag gatgccgcga aattcccgag
     5221 gctcagcatg ggcgagaagg aagtgcggat cgaggaggcc tggcagagcg ccgacccctc
     5281 gccggagacg gccgagaacg aggcccgcat cgagttcctg cgccgcgtgg cgtacgccaa
     5341 ccagcactac accatcttta cgcccggcat gttctccgac cggggacgcg tctacatccg
     5401 ctacggcgag cccgacgaga tgaagatcga gcggctgccg gtcgcgggga agacgatcgg
     5461 ccacgtgctc gattcctcca tcccgcaggc ctcgcgcgag acgatcacgc ggaccgacac
     5521 cgggatcgcc gactcgcggc cctacgagat ctggacctac aactcgcacg gccatgaggc
     5581 ctccgcccgc cacgggttga acgaggtcgc ctccggtctc aagttcgtct tcatcgacga
     5641 ccagggatac ggcgagtaca ccctccgcta ctcctccacg accggaatcc actgagcgtc
     5701 cgggggcatc tccaccccca gggccgcgct tgacttgagc gccggcggcg cctagaatcc
     5761 accgggctct aagtcacacg taacgaagca gttactctga gcttttcgag ggaacgcggg
     5821 ggcgccggat gcggatcgga atgttgacgg gcgggggcga ctgccctggc ctgaacgcgg
     5881 tgatccgcgc ggtcgtccaa cgggccgtca agacctacga ctgggacgtg tgcggcatcc
     5941 gccacgggtg gcgcggtctc ctcgtggacg agatcgacgc gctcgatctc gccaaggtgt
     6001 cgggcatcct gccgcgcggc ggcaccatcc tcggtacctc gcgcacgaac cccttcaagg
     6061 aagccgacgg acccgcgcgg atccgagcgg cgctcgcgac ccacgcgatc gacgcgctgg
     6121 tcgcgatcgg cggcgaagac acgctcggca cggccaacaa gctcgcccag gacggcctcc
     6181 caatcgtcgg cgttcccaag acgatcgaca acgacgtcaa cggcacggac ctcacgttcg
     6241 gcttcgacac cgccctcacc atcgccaccg aggcgatcga ccgcctgcac accacggcgg
     6301 agtcgcacga ccgcgtcatg gtcgtcgagg tgatggggcg ccacgccggg tggatcgcgc
     6361 tcgagagcgg aatcgccgga ggcgccgacc tgatcctgat cccggaattt ccggtctcct
     6421 tctcggaggt gaccgaggtc gtgcgccgcc gccacgaccg ggggaagagc ttcagcatca
     6481 tcgtcgtcgc cgagggagcg cacctcgcgg acgacggcgg ccaggcgctc catctcgcga
     6541 gcgagaagat ggacgccttc ggccacgtgc gtctcggcgg catcggcgcc acgctggcgg
     6601 agcggctcga ggagcagacc ggcttcgaga cccgctacac catcctgggg cacatccagc
     6661 gcggcggttc tccgaccgcc ttcgaccggg tgctcggatc gcgcttcggc atcgccgccg
     6721 tcgacctgat cgcgaagcag ggatatgggc ggatggtcgc cctccgcggc acgcagatcg
     6781 tggacgtccc gctcgccgag gcggtcggga cgctcaaaac cgtcgggtcc gagctctacg
     6841 agctggcccg agtcttctcc agctaaggag gcgctgtgcg cgtcgccatc aacggcttcg
     6901 gtcggatcgg gcgcctagtg ctccgggccg cgctgaagcg gaaggaaacg atcgacttcg
     6961 tggcggtgaa cgacatcacg gacgcgaaga ccctggcgca tctcctcacg tacgactcga
     7021 tccacggtcc ctggcccgag cggttttccg cggccgaggg cggcctccgc gtcggcacga
     7081 cgacgatccg ggtcctgtgc gaagcgaaac ccgagaacct gccgtggaag gacctcggcg
     7141 tggacgtggt gctggagtgc acggggcgct tcaccgaccg cgacaaggcc gcggtgcatc
     7201 tgggcgccgg cgccaagcgc gtgctcgtct ccgccccctc caagggcgcc gatgcgacct
     7261 tcgtcatggg ggtgaacgac aagacatacg atcccgcgaa gcaccaggtg gtctcgatcg
     7321 cctcgtgcac gaccaactgc ctggcccccg tgctgtccgt gctcgacgag tcgttcggga
     7381 tcgagcgggg cttcatgacc accatccacg cctacacgaa cgaccagaag gtgctggacg
     7441 cgccgcacaa agaccttcgc cgcgcgcgct cggcggcgca gtcgatgatc ccgacctcga
     7501 ccggcgccgc caaggcgatc gggctcgtga ttccctcgct gcaggggaag ctggacggca
     7561 tcgcggtccg ggtgccgatc ccctgcgggt cgatcatcga cgtcgcggcg gagacggcac
     7621 gcccggtgtc ggcggcggcg gtgaacgacg ccatgcgcgc cgcctcgacg gggcggctct
     7681 cggggatcct ccagtacatg gaggatccga tcgtctcggc cgacatcgtg ggcaatccgc
     7741 attccgcgat cttcgacgcc ccgctcacca tggcccaggg ggaccggctg gtgaaggtct
     7801 tcgcctggta cgacaacgaa tggggcttct cgaaccgcat ggtggacgcg ctgctcctct
     7861 tggggaaggc gtcctgagga gaacgacatg acgttccggc ggctgaacca tctggaggcg
     7921 gaggggcgga cgatcttcgt gcgcgtcgac ttcaactgcc ccctgaagcc cgaaggaggc
     7981 gtggccgacg acacccgcat cgtggcctcg ctgccgacgc tgcggtacct gctcgacaag
     8041 ggcgcgcgcc tcgtcgtggc gtcgcacctg gggcgcccca agggaaagcg cgatgcgaag
     8101 cagagcctga tgcccgtgcg ggaagtgctc gagaagtatc tccgagggcc cgtgcgcttc
     8161 gccccctcga tcatcggacc cgaggcggaa gccgaggcgc gcgcgctcga gccgggacag
     8221 gccctcctgt tggaaaacct gcgtttcgac ccgcgcgagg aggcgaacga tcccgagttc
     8281 agccgcgccc tggccgcgct cgccggggcc tacgtgaacg acgcgttcgg ctcggcgcac
     8341 cgcgcgcacg cctcgaccgt cgggatcacc gcgtacctgg ccgatcgcgc ggccggattc
     8401 ctgatggaga aggagctctc ggcgctggga cggctcttgg aaggggccgc gcggccgtac
     8461 gtcgcgatcc tcggcggcgc caagatctcg ggcaagatcg acgtgctcca gaacctcctg
     8521 ccgcgcgtgg accgggtctt cgtcggaggc gggatgatgt tcaccttcct gcgcgcgaag
     8581 ggactggaga ccgggcgatc gctcgtcgaa gaggatcggg tcccgctcgc ggccgagctg
     8641 ctccggtcgg cggagggctc gaagctgctc ctgcccgagg actgccgcgc cgcggccgac
     8701 gtgaaggggg aagacccggg acgcatcgtt cccgtgacgg cgattccgtc cgaccggatg
     8761 ggcgtggaca tcggccccgc gtcgcttgga gcggtcggtt cggcgctcgc cgaggcgaag
     8821 acggtgttct ggaacggccc catggggatc ttcgaagtgc cgacctatgc cgaagggacg
     8881 atcggcgtgg cgcgcgagat ggcccgcgcc accaagcgcg gcgccttcac cgtcgtgggc
     8941 ggcggcgact cgctcgccgc ggtgaacgcg gctgaggtgg agcaccagat cagccatctt
     9001 tcgacgggcg gcggcgcgtc gctcgagttc ctggaaggga aggagcttcc cggcgtcgcc
     9061 gcgctggagg acgcgccgcg gccggcacga tgaagcgcat cctggccgga aactggaaga
     9121 tgaacctcct cctggccgag gcgcgcgccc tctacgagga ggtcgccgcg ggatgccggg
     9181 agcgctcggg aatcgcctgc gtcctctttc cgcccgcgac cgcgctcgcc gcgctggcgg
     9241 cggcgcggac ggaggaaggt cccgcgctcg gggtgcagaa ctgccacttc gagtcgaagg
     9301 gcgcgttcac cggcgaggtc tcggcggagc aggccaggga cgcgggcgcg cggtacgcgc
     9361 tggttgggca ctcggagcgg cgccggctct tctgcgagag cgatcagctc gtgcgccaga
     9421 agctgatcgc cgcgtggcgc acggggctcc atcccgtgct ctgcatcggc gagaccctgg
     9481 ccgagcgcga gcggcgcgag acgcgcgacg tgctcgcgcg tcagctccgg cgcgcgctcg
     9541 aaggctctcc cgggaaagcg ccgctctggg tcgcgtacga gccggtgtgg gcgatcggaa
     9601 cgggggtggt cgcgtcggtg gaggaggtcg tcgaggccca cgcctgggtg cgcgaggagc
     9661 tcgcggccgc gggacggggc gccgccggcg atccggtccc cattctctac ggcgggagcg
     9721 tggaccccaa gaatgccggg ccgctggccg cggcgccggg ggtggacggc ttcctcgtgg
     9781 gcggggcctc gctgcaggcc gcctccttcc tcgagatcgg ccgcctgctg gagcagaccg
     9841 tccccgcgca ttgaccctcg cgggggcgcg tggtaccctc ctttactcgt ttcggcacgg
     9901 caaacgatct cgaaaggaag ctcgcgcatg tccggtgtca tcctgggtct ggtcgtcacc
     9961 attcacgttc tcgcctgcat cgcgctcatg atcacgatcc tcctccagtc ggggaagggc
    10021 ggcggtctgg ccggcgcgtt cgggggcggc tccagccaga ccctcttcgg cggacgcggc
    10081 gcggcgggct tcctgagccg cgcgaccacc gtgctcgcga tcctcttctt cgtgacgtcg
    10141 ctgaccctcg gcctctcgtc gtcgaaggcc acgtcgcggc gcagcctggt tcaggaagag
    10201 gcgcggcgcc gcgcggcggg cgcgcctccc gtcggttcgg gcgcgaccgc gccttcgccg
    10261 ggggcgtcca gtcccctggg cggcggcacc gtgacgcccg ctcctcaggg tggcggcatg
    10321 gcgcccgctc ctccgagcgg cacggctccg gctccacgcg gcacgacggc tccctcgccg
    10381 agcggtacga cggctcctcc ttccggtccc gcgacgagcg gcggcgcgac gacgccaccg
    10441 gcctcgggca gcggacagtc gcccgcgacc ggcggacaat gatccagcag acgcccgggt
    10501 ggtggaatct ggtagacacg tacgtttgag gggcgtatgg gtaacccccg tgcgagttca
    10561 aatctcgccc cgggcacaca tcgggtcccc ttcccgcgag ggagggggac ccgttttcat
    10621 gcgcgagcgc gtcgtggcgc gagtggacag gctcgcgcgg gcccttgcgg cgtctctagg
    10681 ggaccgggtt ctccggagtg gacattgcct gagcgcctgc gcgcgaagcc gccggctcgg
    10741 gaaggatccc gaccaggcgc atgcagaagc gctcttccgc gcggatctcg ttccgtccgc
    10801 cgcagtgctg gcagcggatg taggcccggc gcggatcttc cttcaggtgg aagatctgat
    10861 tcgggccgaa cagcctgctg cacctcaggc actcgaccgc ggggctggag acgatctcgg
    10921 acatctcggg cattcggact cctccatcgg gagagggaag ggggcgcggc gcgtccgcgc
    10981 ggcgcatcgt tcgacgcgta ctatagcccc ggcgttccgg agcggcaagc gggcggggga
    11041 gcgcgtcgcg tgaccggccg cgctcccgaa accggctacg acgcggctcg cgccgccgcc
    11101 tattggggcg gggcgcggca cgagacggga gacgagctgg ccgcggtgct cagcctgggc
    11161 gagccgccgc acgtgaacgc ggcgtacgac gcctgggaga cggggctcct gatggacgtg
    11221 atcggcagcg ccgaccgcgg cctcgacctc ggcgcgggcg tgggaagggt atcgatccgg
    11281 ctttcgtcgc gggtggcccg cctcgcctgc ggggacctgg cgccgggaat gctcgagcgg
    11341 ctgcggcgga acgcggcgcg cgccggtgct atgggcctcg acccggtgcg gctccgctcc
    11401 gaccggctcc cgtttcgcga gggctccttt ccggtcgcgg tctgcctcgg gctcctggaa
    11461 catttgcccc ccgaggcgcg ccgcgccacg ctggccgagc tggcccgagt cgtgcgtcct
    11521 ggaggactcc tcgtgctcgt cctgaacaac gaacgaagcg cgttgctccg ggatccctcg
    11581 gacaaccccc accgcgtcgg cgcgcagcgc gagaacggct actactgcgc cgtggtgccg
    11641 gaggcggcac tcctggacga ggcaaagccc catttccgtg acgaggcgct cggctccaat
    11701 ctcttctacg cgctgcagcg ccacgcctcg cgcgccctgg acgaacgccg gcggagggac
    11761 gcgcgcctcg cccccttctt cgagcgcgcc gcggcgtggg accgcgcgct ccggccgcgc
    11821 gggccgatgg cggacgccgc ggccgaccac caccttcacc tcctggtgcg ccggtgagcc
    11881 ggccgccgat cctggtcacc gggggcgccg gctacatcgg ctccgtgctc gtgcgccgtc
    11941 tcctcgagga cggggcccgc gtgcgcgtcc tcgaccgatt cctcttcggc ggccagggtt
    12001 tggatgggat cgcctcggac ggatcgctca cggtgctcca ccgggatctt cgcgatccgc
    12061 gggcgcacgc cgaggcgctc gagggcgtgg agacggtgat ccacctcgcg gcgatcgtcg
    12121 gggacaaggc gtgcgcgcag gacgaggagc gggccgtgca gaccaactgg accgcgacgg
    12181 tggccttcgc gcgatccgcc gcggcacgcg gcatccgccg cttcgtcttc gcgtccacct
    12241 gcagcgtcta cggcgagggg cgcgaggaga ccctcaccga ggagtctccc gtgaagccgc
    12301 tctccctcta cgcggaaacg cgctggcatg ccgagcaggg gctcttggag ctggcctccg
    12361 agggaacgct ccagccggtg ctgctgcgct tcgggaccgt ctacgggctg tcgccgcgga
    12421 tgcgcttcga cctggtggtg aacctgctcg cgcgcgacgc cgtgctgaag ggcgccgtca
    12481 ccatcttcgg cggcgatcag tggcgcccct tcgtgcacgt ctcggacatc gcgcgcgcgc
    12541 tccggctggc cgcgttcgag ccccttccgg aagactcgcc gatcgtgaac gtgggggaca
    12601 acctcgagaa ctaccagctc aaggacctca aggaggcgat cgaggagaac gttcccggcg
    12661 ccagcgtcac gatccagccc gccgccaagg acccccgcac ctaccgcgtc tcgttcgaca
    12721 agatcgagcg gctctggggg ttccgcgccg agcgccgcgt cggggagggg atcgccgaga
    12781 tcgccgcggc ggttcggggc ggccggatcc cccagcccta cgacgcccgc tacgtgaacg
    12841 cctgatggtt ccgtggtata cgcctcggga tttcccttgc gcggccgggg gccgccccgg
    12901 tagcgtcgga tcgttacctc gtccgacttc cgggagacaa acccatgccc cgtttggaat
    12961 tcgacccgac ccagctccgc gagcgcgcgc ttttcgtgcg ccgcgacatc atcaccctgc
    13021 tcgcgcgctc ccagtccggc catagcggcg gaccgctctc ttccgcagac ttcgggagcg
    13081 tgctcttctt ccgggagatg aatctggatc ctgcccggct cgacgacccc ggccgcgatc
    13141 tctggcactt ctcgatcggt cacatcaccc cctgcatcta cgcgctgatg ggggagcgcg
    13201 gctacttccc gcttcgcgat ctcctccagt tccgccggtt cgagggacac ctccaggggc
    13261 atccgagcaa gcacgacacg cccggcgtcg aggtatcgtc cggctcgctc ggccagggtc
    13321 tctcggtctg ctgcgggatg gcgctcggct cgcggatgga caagaacccg cggcgcgtct
    13381 actgcgtgat gggcgacgga gagcagcagg agggtcagat ctgggaggcg gcgatgttcg
    13441 ccgcgcacta caagctggac aacctctgcg gcgtgatcga ctacaaccgg aagcagatcg
    13501 acggcgacgt gcaggacgtg atggggatcg cgccgctggc ggacaaatgg cgcgcgttca
    13561 actggaacgt gatcatctgc gacggccacg acatcccctc catcatcgac gcgttcgacg
    13621 gcgcgcgcgc gacgaaggga aaaccctcgg tgatcctcgc caagacggtg atggggaagg
    13681 gcgtttccta catggaagac gactaccgct ggcacggcgt gccgcccagc gtggagcagg
    13741 cggagaaagc gctcgcggag ctgggaacga ccctggaggc ctggacgaag cgtctggagt
    13801 cccacggcgc gccgcaggtg gcgccgtccg cggctcccgc gacgccggta ccggccgccg
    13861 tggcgggagg tgcccgatga gcagcggaac cgctccggcc gccccgcgtc acgccgaacc
    13921 cgcgaccttg gccaagcccc agatgaagcg cacccgcgag gggttcgggc acgggctggt
    13981 ggacctggga gagaaggatc cgcgcatcgt cgtgctggtc ggcgacctga ccggctcgac
    14041 gaacgtgaac ttcttcgccg agaagttccc cgaccgcttc attcaggtcg gcatcgccga
    14101 gcagaacatg atgtgcgtag ccgccggcct cgccgcggtc gggaagattc ccttcctcgc
    14161 gacctacggc gccttcgcct cctgccggag cgcggatcag atccgcgtga ccgtggccta
    14221 cacgaatctc ccggtgaaga tcggaggcgc gcacggcggc atcagcgtgg gtcccgacgg
    14281 ggccacccat caggcgatgg aggagatcgc gatcatccgc tcgattccca acatgcggat
    14341 gatcgtcccc gccgactacg acgagacgcg caaggcgacg gtggccgcgg ggagcgacct
    14401 gcatccgtac tacatccgct tcgggcgcga gccggtcccg gtcgtgaccg acgccgggtc
    14461 tcccttcgtg atcgggcgcg cgaaccagct ccgcgagggg aaggacctgg cgatcatcgc
    14521 gtgcggcgtg atggtctacg aggcgctcgt ggccgccgaa gcgctcgcgg cgcgcggcat
    14581 cgagtgccgc gtgctgaaca tgcataccct gaagccgctg gacaaggaag ccatcgagcg
    14641 cgccgcgcgc gattgcggcg ccatcgtcac cgccgaagaa caccaggtcc acggcgggct
    14701 ggggggcgcc gtcgccgaag ccgtggtggc cgcccatccc gttccgatgg agctggtggc
    14761 ggtgcacgac cgcttcggcc ggtcgggaaa gcaggacgaa ctgatggccg cgttcgggat
    14821 caaggccccg gacgtgatcc gggccgcgga gcgcgtgctc gctcgcaagc gcggccggta
    14881 atgccgcggc gccacgccgc gccacgttca tgagagtccc gctccgggcc gcggccacgg
    14941 cgacggcgct catcgctgcc ctggcctcgg tcgcgccgtt cacgcacgcc cagacgccgc
    15001 gtgaagcgcc gccgcccacg tccctatcct caaccgatac ccttgccgtt cccgtccgcg
    15061 tcgtcctggg acagggtccc gccttcgccg tggccgcgtt cgattccgcg accggttcct
    15121 ggggcgcggc gtgcgccgcc tcggagatcg cggtgggcgc gcgcgccctg gaggcttcgg
    15181 ccgagacagg tgccttgctc tcgctgggga ccggcggcgg ggacctgggc gccgcggcca
    15241 ccgcgctcgc gcgagggatg gatccggaca ccgccctggg cctgctcgtg ccttccggca
    15301 ccgatccggc cggccgcctg ggcatcgtcg tggcgcgcgg cgggcgtgtc gcggcccgct
    15361 cgggagcgca cctgccgggg ttttcgggca cggtccttcg cccgcgcttc gcctgcgccg
    15421 gctacggcct ccggggtgcg gcctccctcg aggcgatgga cgaggcgttc gcgcagcgag
    15481 ggggcgatct gggcgcgcgg ctcctcgccg cgctcgaagc gggcgagcgc gcggacggcg
    15541 accccttcgc ggccagggga aaggaagcct cggcggcgct cctcgtcgtg cgcgccgtga
    15601 cggagtccgg gtccggcacc ggcaccgacc gagtcaccga tctgcgcgtg gacctggaca
    15661 aggatccgat cggggccctg ggccggatct acgcgcgcca tgccgagacg ttccttcccg
    15721 cggcacacgt ccgctacggc gacgccgcca ggcggcgcgg cgacgatgcg gcggccgcgc
    15781 gcgagtacgc cgccgcggaa gccgggttcc gcgccgcggt cgcccgcggg cccaaggacg
    15841 ccgacgccct gaacgagctg gcgtggttcc tcgcgacgcg cggcggcgat cccgtcgagg
    15901 cgctgcggta cgcggaagcc gcggtgggga cgcgcagcga cgatccgaac ctcctcgaca
    15961 cgctggccga ggcggcctac cgcgcgggca atctcgagcg ggcgatcgaa gcggcggagc
    16021 gcgcggtgcg gctgtcgcgg gggaacgagc gctacgcgga gcggctccgc gcgttccgcg
    16081 ccgcgcgagc ggcgctgact ccacctagcc ggtaaacgag ccgggtgcgg gctggggcgt
    16141 ccggagaaac ggggccagcg cgcggactcc gatcggctcg gtggcgtgga agggctcgcc
    16201 gtaggtcacg ccgatcatcc cgccgtagtg gcgcgcgcgc gattccacga agtcgaggaa
    16261 accctcccga ttgagcggcg tgggccgcgc gtccgccgag gcgcggctca cctggcttcc
    16321 atgcgcgagg atcgccgcgc gcttcgcctc ccagtgggcc gtgacgtcca cgacgaacga
    16381 aggcgcgaag ccgacgcgcg aggcgaagtg gtagcgcacg cggggacgga agggagcggc
    16441 gtcgccgccg ccctcgaagc gccgcagccc ggccaggtag gaggcgcggt cgatcagctc
    16501 cgatgcggcc acgtgatcgg ggtggcggtc cttggggaag tgcacgacca ccacgtcggg
    16561 gcggagcgcg cgaagccagc gcaccgcgcg gcgcacctgc tcgggatcgt gggcgtggag
    16621 gccgccgtcg ggcagccccg cgttccaccg gccgcacgcg tgcagaaggc gggcggcctc
    16681 cgccgcttcg gccgctcgaa tctccggggt gccgttggtc gcgctctccc cgcgcgtcag
    16741 gtccaggatt ccggcgcgca gcccctcggc ggccgcggcc gcgatcgtcc cgccgcagga
    16801 gatctccacg tcgtcgggat gcgcgccgat ggcgagcagg tcgagcggag gtccgtcgtg
    16861 cgaggaggga agatcgaggt cgcgcgcggg cgccacggga tcaggaccgg tggaaggctt
    16921 cgaacggctc gatgctcacc ggacggccgt ccggtccgga gaagcggtgg ctggtccgga
    16981 ggatgcgcac cgtcccggtg gccgcgcgca tcaccatcga gtgggtctcg gcgccgtccc
    17041 cgctgaagcg gacgccgtcg agcatgtccc cgtcggtgac gccggtcgtg gcgaagatga
    17101 tcgagtcgcc ccgcgcgaga tcggtctcgc tgtagatccg gtcgtggtcc gtgattccca
    17161 tccgcttcgc gcgctgcttc tcctcgtcgc tccggaactt gaggcgcccc tgcatcgcgc
    17221 cccccatgca gcggagcgcc gcggccgcga gcacgccctc gggcgcgccg ccgatcccca
    17281 ccagcacgtc cacgcccgtc gactcgaccg cggtggccat ggcggccgag acgtcaccgt
    17341 cggggatcag gtggatgcgc gcgccgacct tccggatccg ctggatcagc tcctcgtggc
    17401 gcggccggtc gagcacgacg acggtgaggt cctccaggtc gtagcccttg gcggcggcga
    17461 tcgactcgag gttttcctcg acggagcggg agagatcgat cgcgttcgcg gccttggcgc
    17521 cgaccgcgat cttctccatg taggtgtccg gggcgtggag gaagtggccc ttctgggcga
    17581 gggcgatgac cgcgagggcg ttgtcgcggc cgaaggccac cgagttcgtg cactcgagcg
    17641 ggtcgagcgc gatgtccacc tcggggccgt accccgagcc cacgcgctcc ccgatgtaga
    17701 gcatgggtgc ctcgtcgcgc tctccttcgc cgatcaccac cgtgccgcgg atgtccacgt
    17761 tgtcgaacgc gcgccgcatc gcggtcacgg cggcctggtc ggccccgtgc gcgtcgccgc
    17821 gccccatgaa gcgcgcgcag gcgagcgcgg ccgcttcggt gacgcggacg aggtcgaggc
    17881 cgacgtggcg gtccactagc gggcctccgg gccggtcagg gaggtggcgt cgatctcctc
    17941 ccagtcgttc cgcgcgtcga tccagcggaa gaggcggatg cggctggtgg actccgtctt
    18001 ggaatagacg tactcgacgt gggtgcctcc gccggcgcga ttcgagtagt gcatcttctt
    18061 gtcgacgacg agcggcttcg tggtgcggac cagcttcatc cggccctggg ggctctccca
    18121 cgtgacggat tcggagacgg cgtgcgtctc ctcgtcctcg gactcgtcga attcgaggtt
    18181 cccgaagtgc ttgtccaggc ggcggagcac ctcttcccag cgctggtcgt tcatggatcg
    18241 gccctcgatt gacttgcgga ctgagccgcc tatagtacca cagcccatgg ccccgacaga
    18301 agatgccccg ctcgcttccg gattccaggg acgctccgcc ctcgtcgtcg ggggctcccg
    18361 cggcctcggc cgtgccgtgg cgatcgcgct cgcgcgcgag ggatgccgcg tgctggcggt
    18421 ggggcgctcg gcggcgccgc tcgccgcgct gcgcgagacg gccgcggcgc gggggctccg
    18481 cctgcgcacg gcgcgcggcg acgtgacgcg gcccggcacg gcgcggcggc tggtgcgcca
    18541 ggcgggcgcg ctttccgagg gcgggggtcg cggccggctg gggcacgccg cccccgacat
    18601 cgtcgtgcac gccgcgggcg actactggga agggccgctt cgggccctca cgcccgagcg
    18661 gtgggaggcg ctggtccact ccaacgtgac cggcgcggtc gcgcttctcc acgccgcgct
    18721 tccacccatg cgccggcggc gcttcgggcg cgtcctcctc ttcggcgtcg ccggagggga
    18781 catgccgcgg gcggcgtcgc gcgcgcacgg ctaccgcgcc gccaagatcg ccctcctcac
    18841 gatcgcgcgg agcgcggccc aggaggaggc ggagcacggc atcaccatca acgtgatcct
    18901 cccgggggtg atccggactc ccggcacgcc ggcgcggtgg gccgcggccg ccgccgcggc
    18961 gaagatcccg gcccgacgcc tggggacgcc gggggaggtg gcgcgcgccg cgctcttcct
    19021 gctggcggag gaatcggggt acatcacggg cgccgcgctc ccggtctccg gcggatacct
    19081 gctctgaccc tccgcggagg ggcatccgta actccctgga gccccggccg ttgctgacga
    19141 tcgcgcaagg tgttagaatc aacgtggctc catgctcccg aatggctgtt tccgtgtccc
    19201 cgagggtgac ctcttgcgcc cgtccacgcc caagacctgg gtcctgaaaa gccccgcggc
    19261 ccccgagctc gcggaacggc tgaaggccga attgggcgtg tccgatacgt tcgcccggct
    19321 gctcgccaac cgcgggttca cctcctccga gcaggtcgag tcgttcctcg ttccctcgac
    19381 cgacaagctg ctcgacgcgt tcaccatgcg cgacatggac cgcgcggtgg accgggtgct
    19441 gcaggccgtg gccgcgcgcg agtcgatcct cgtgtacggc gactacgacg tggacggcat
    19501 cacgtcgacg tcgctcctga cggccgcgct ggcgaagatg ggcgccaagg tctcctactt
    19561 catccccgac cggatccggg acggttacgg cttctcggag cggggcgtgg acgtggcgcg
    19621 caagcgccgc atccgcctcg tgatcaccgc cgactgcggc atcaccgcca cgaacgaggt
    19681 gcgcctcgcg cagaaggagg gcatcgacgt catcgtgacc gaccatcacg agccgctcgg
    19741 cgagctgccg caggcttccg ccgtgctgaa ccccaagcgc aaggactgcc cgtacccgtt
    19801 caaggagctc gcgggggtcg gcgtggtctt caagctggtg caggggctgg ccgcgcggcg
    19861 ccccgacgtg ctcccgcccg atttcgtctt ccaccatctc gacctggtcg cgctcggcac
    19921 gatcgccgac gtcgttccgc tggtggggga gaaccgcgtc ttcgccaaga tcggcctgga
    19981 gcgcctctgc cattccgaca agcccgggat cgccgcgctc aaggaggtgg cgggactccg
    20041 cacgcgccgc gtcgagagcg gccacgtggc ctacatcctg gcgccgcgca tcaacgccgc
    20101 cggaaggctc gggaacgcgg agagcggcgt gcgcctgctc ctctcgaacg atccgcgcga
    20161 agcggcgatc atcgcggaga gcctcgagga ggacaacgcg aaccggaaga agatcgacga
    20221 gtccacgctg gaggacgcgc tcggccagct gcagaagcac ggcagcgagc ttcctcccgc
    20281 catcgtcctg tggtccgacc ggtggcatcc cggcgtgctc gggatcgtcg cgtcgcggct
    20341 catcgagcgg ttccaccgac cgacgatcct catcgcctcc gacggggacg aagggaaggg
    20401 ctcggggcgg agcatccccg gattcgacgt ctgcctggcg cttcaggagt gccgcgcaca
    20461 cctcctcggc ttcggcggcc actcctacgc cgccggcctc acgatccgga ccgagtgcct
    20521 cgaggcgttc cgcgaccacc tctgcgacgt ggtgcgcacc aaggtccagc ccgacgattt
    20581 catccccaag ctctcgatcg acggcccgat cgatctggag cacctgaacg aagatctggt
    20641 ccaggcgctg gaccggctcg cgccgttcgg gatcggcaac gccgagccgc tcttcgtggc
    20701 cgacgacgtg aagctttccc tgcctcccgc cgtcgtgagc cggaaccacc tcaagctcac
    20761 catccgccag aacggccgcg acctcgactg catcgggttc ggcatgggac acatggcgag
    20821 cgtgatcgcc cagaacggtg gcaagctctc cgtggcgttc gtccccacga tcaacgtctg
    20881 gcagaaccgc tcgcgcctcc agctcaagct gcgcgacatc caggtccgct gaccgtggtc
    20941 ccggcccccc cggacgcctt ggggccgctc ctcgaggcgc tgcacaagac cggcaaaccc
    21001 ttccatctcg agaaggtccg cgaggcctac gaatttgccg cgcgcgcgca cgccaaccag
    21061 aagcgccgct cgggcgagcc gttcgtcacc cactcggtcg cggtggccgc gatcctcgtg
    21121 gatctcctgg ggaccggact cgacgacacg ctgctcgaag cggcactgct ccacgacgtg
    21181 gtggaggaca cggagatcga cctcccggcc atccagcgcg cgttcggtcc cgaggtcgcc
    21241 gccctggtgg acggcgtgac caagatcagc gggctccact tcgaccgccc cgagacggag
    21301 caggcggaga acttccggaa gatgctcctg tccatggcgc gcgacctgcg cgtcatcctg
    21361 atcaagctgg cggaccgcct ccacaacatg cgcaccctga acgcgctgaa tcccgagcgg
    21421 gcgcgcgcca tcgcgcgcga gacgcgcgag atctacgcgc cgctcgccca ccggctgggc
    21481 atcgcgcgga tcaagtggga gctggacgac ctctgcctga agcacctcga tttcgaggcg
    21541 ttccaggagc tccgccagaa ggtggcgctg aagcgcgagg agcgcgagcg gcagatccag
    21601 gaggtgatgg ggccggtgaa ggaccgcctc gtggcggccg gcatcaaggc ggacgtcatg
    21661 gggcgcccga agcacctgga gtcgatctac cggaagatgc gcacgcagga gcgcgccttc
    21721 gaggagatct tcgacctcct gggcatccgg gtgatcacgc gcgacaaggc cgagtgctac
    21781 cgggtcctcg gcgtggtgca cgacctcttc acgccggtgc acgagcgctt caaggactac
    21841 atcgccacgc ccaagagcaa catgtaccag tcgctccata cgacggtggt cgcgcccggc
    21901 ggcctcatgg tggagttcca gatccgcacc gccgagatgc accagatcgc cgagctcggc
    21961 gtcgcggcgc actaccggta caaggagggc ggacggcagg acgcggagct cgaggcgaag
    22021 ctggcgccgc tcctgcgcgg ggcggacttc cagagcgcga cgacgggcga ccccgacgag
    22081 ttcatggaat acctcaagac gagcctctac caggatgagg tcttcgtctt cacgccgcgg
    22141 ggcgacctgc ggcggctgcc gcgcggcgcc acaccgctcg acttcgccta cctcgtgcac
    22201 acgcaggtgg gaaaccactg cgtcggcgcc aaggtgaacg gccggatcgt gccgctccgc
    22261 accgagctcc ggagcgggga cacggtgcag atcatcacct cgccgggcgc caagccgaac
    22321 cgcgactggc tgaagctggt gcgcacgcac gccgcgcgct ccaagatccg ccactggctc
    22381 aaggtggccg agcactccga cagcgtcgcg ctcggaaagg agatcatcga gcgcgagctc
    22441 aggcggcggc gcctgacccc tcccgccggc gtctccctgg agagcctcgc gcagtcaatg
    22501 ggccatcccg acgtgaacgg gctctacgcc gccctgggtc agggcaatct ctcggtgcag
    22561 caggtgatcg gcaaatggat ccccgacggc ggcggcccgg tgcagcgcct caaggaggcc
    22621 tcgctcgaga cgatccgtgc gatcacgggg cggccggggc acggcgtgcg catccagggc
    22681 gtggacaacc tgatggtcgc ctacgccaag tgctgccagc cggtgccggg cgatccagtg
    22741 gtcggcattg tgacgcgcgg gcgcggggtg accgtgcacc gcaccgactg cccgaacacg
    22801 ttcgacgacc gcgtgcccgc ggagcggcgg atggtggtgg actggagcgc caagcccgag
    22861 gactccttca ccgtgaagct ctccatcttc ggccaggacc ggaagagcct cctggccgac
    22921 atcgccaagg cgatctcgac caccaacacg aacatccgga ccgccgggat caaggcgaac
    22981 gaccggaacg cccgcggcgc gttcgtggtc gaggtgcgcg acctggcgca tctgcgcgag
    23041 gtgatgcgcg cgatcgagaa cgtggacggc gtggacgccg tcgagcgcga gcaggtcttc
    23101 ggcaagccgg gccacgggga agccggcctc gcttcgtgag ggcgctcctc cagcgcgtca
    23161 gccgggccgc ggtccgcgtg gaggacgaag agatcgcgcg gatcgggccg gggctcctga
    23221 tcctcctggg ggtcggcgcg tccgatccgg acgccgcggc gccccgtctc gccgagcgct
    23281 gcgccctgct ccgcatcttc gaggacgagg cgggccggat gaatcgctcc gtgatcgacg
    23341 tggcgggaga ggcgctggtc gtatcacaat tcacgctggt cgccgacgtg tcgcggggcc
    23401 gccggccggg gttcgacccg gccgcgccgc cggcggcggc cgaggcggcg tacgagcgct
    23461 tctgcgccgc gctggagggg gccggagttc cggtccgccg cggacgcttc ggggccccga
    23521 tgcaggtcga gctcgtgaac gagggcccgg ccacctttct cctcgagacg ccgtcgtgat
    23581 tccggaggag gtccggcgcg catgcgcccc aatctgctga atccactggg ccccaagctc
    23641 cggctcgcgt cgcgctcgcc gcgccgcgcc gagctgctca cgctggtggg ggcccacttc
    23701 gagatcgctc ccgtcgagct ggacgagagc gcgctcccgg gggagcgccc cgacgctcac
    23761 gtgctgcgcc tggcggaggc gaaagcgcgc gcctcgcgcg cggcgcgcga cgcggccggc
    23821 gctggacagg atgcgggagc cggcccggcg ggggacggcc ccgagggaac ggtctacgtc
    23881 ggcgccgaca cggtcgtcgt gatcgaccgc gtcatcctgg gcaagccggc tgatcgcgcg
    23941 gacgccgcgc gcatgctggg cctcctgtcg gggcgcgtgc acgaggtctg gaccggcctc
    24001 ttcatcctcg atccagccga ggagcggggg ctgggcgagg cggtgcggag catcgtcaag
    24061 ttctcgccca tcggcgccgc gatgatcgat cggtacgtcg ccaccggcga gccgctcgac
    24121 aaggcgggcg cctacgccgt tcagggggga ggcgctctct tcgtggaggc gatcgaggga
    24181 tcctattcga acgtggtcgg gctcccgctc agccacctga agcatctgct cgggctgctg
    24241 cgcgaagcgc cggtccggga gccctgagcg gaccgcatgg atcctctccg gatcgagaag
    24301 ggcgcgctcg aagtcctcga ccagacgctg ttgccggaac gggaatcgtg gatcgcggtc
    24361 gacggtgccg cggccatggc cgaggcgatc gcgcggctcg ccgtccgcgg cgcgccggcg
    24421 atcggcatcg ccgccgcgct cgcgctcgcg gccgaggcgc ggcgccgcgc ggccgcctcc
    24481 tccgacgcgg cgccgctgtt ggcggcgctc cgcgcgtcgg gcgcgctgct tcgcgcgacg
    24541 cgccccaccg cggtcaatct cgcgtgggcg gtggaccgga cgctcgcggc gtgcgcgtcg
    24601 gccgcggccg agggggccgg cgcgggcgcg atcgccgagc gcgcgcagcg ggaagccgag
    24661 gcgatctggg aggaggaccg cgccgcttcg cgcgccatgg ccgcgcatgg cgcgacgctc
    24721 ctcggatcga ggcggcggtt cctcacgcac tgcaacaccg gagggctggc gacgggaggc
    24781 ggcggcaccg cgctcggcgt gatcctcgag atgcatcgcc gccaccccag cgcggtcgag
    24841 gtgtggcaca cggagacccg tccgctcctc cagggcgccc gcctcaccgc ctgggagctc
    24901 gtgcgcgccg gggtcgtgcc gcgcctgatc gccgacggcg cggcggcgca cgtgatcgcc
    24961 cgggagggga tcgaggcggt tctcgtgggc gccgaccgga tcgccagaaa cggggacgcg
    25021 gcgaacaaga tcggcacgta tgggctggcc ctggcggccc atgcgtcggg catccccttc
    25081 gtcgtggtgg ccccgacctc cacgatggat ccctcgctcg aggacggcgc ctcgatcccg
    25141 atcgaggagc gggattcctc ggaactgacg ctgttgcggg ggattccgac tgcggcggcg
    25201 ggggtcctgg ggcggaatcc ggccttcgac gtcacccccg cccggttcat ctcctgggtg
    25261 gtgacggaac ggggcgccgc ccgccccggc gagaccccgg ccgggatggt tgacagagtg
    25321 gtgtaagcgc ccgtatcctc tagcgtttgc tcgttccaac aatcgtcagt ggaggtcgaa
    25381 tcgatgggca cgtactcggt acgcgcctcg gagatccagc aggactggta cgtcgtggat
    25441 gccgaggggc agatcctggg ccgtctggcg agtgagatcg cgagcgtgct caagggaaaa
    25501 cggaaaccga cctattccac gcatctcgac gtgggcgaca acgtgatcgt cgtcaacgcc
    25561 gagaaggtgc tggtgacggg acggaagcgc gacaacaaga tctacttccg ccactcgctc
    25621 tatccgggcg gcgacacgcg gacccggttc cgcgacctcc aggcgcagaa gccggatgag
    25681 atcatccgcc tcgcggtgaa ggggatgatg ccgaagaacc ggctcggacg cgcgatgatg
    25741 aagaagctca aggtctacgc cggatcggaa catccgcacg cggcccagat gccgcgtccg
    25801 ttcccgatgc ccggcgcgac gacgggaggt gcttgatggc caatccagtc atggcgaccg
    25861 gacggcggaa gacgtcggtg gcgcgcgtgc ggctggtccc gggcaacggg aagcgtacgg
    25921 tgaacggcgt ggaattcgac cgctacttct gccgcatggt gctcacccgg atggccacgg
    25981 agccgctcct cgcgacgaac ctggccgaca agtacgacgt catcgcgctc gttcgcggcg
    26041 gcggtccggc cggccaggcg ggcgcggtgt cgctcggcgt ggcgcgcgcg ctccaggtga
    26101 tcgacccggc gctccgcggg ctcctcggca aggaggggtt cctcacgcgc gacgcgcgca
    26161 tgaaggagcg gaagaagtac gggcaggcgg gggcgcggaa gcgcttccag ttctccaagc
    26221 ggtaaacgag ctgtcccgcg gctcccgtcc gggagccgcc actacacacg ccccgggcct
    26281 cgggcggtct ccctcgcgcg agggggcccg taggatcgaa ggggcggagg cataagaccg
    26341 gaggtttgga cgtggctgac attggaatga aggagctgct cgaagcgggt gtccacttcg
    26401 ggcaccagac ccgccgctgg aatcccaaga tgaagaagtt catcttcatc gagcggaacg
    26461 gcatctacat catcgatctc cagaagacgc tgaaggcgat ccaggacgcc cgcgcgctcc
    26521 tcgagggcgt ggcgcggcgg agcggcaccg tgctgttcgt cgggacgaag cgccaggcga
    26581 aggacgcggt gcgcgaagag gccgagcggt cgggcatgcc gtacgtgaac gagcgctggc
    26641 tgggcgggat gctcaccaac ttcaagacga tcaagtcgaa catccgccgc ttcaaggaaa
    26701 tcgagaaaat ggacgccgag ggcgtcctgg agaagctgtc caagaaggag caggcccaga
    26761 tcaacaagga gcgggtgcgc ctcgtgaaga tcttcacggg gatcaaggag atggggcagc
    26821 tgccggccgc cgtcttcgtg atcgacacca agaaggagcg gatcgccgtg gccgaggcga
    26881 accggctggg catccccgtg atcggcgtcg tggataccaa ctgcgatccc gacgtgatcg
    26941 acttcccgct gccggggaac gacgacgcga tccgggcgat ccggctcttc agccgtttcg
    27001 tctccgacac gctgatgtcc acgcgccagg agatgcagga gggcgcggac cgcgaagcga
    27061 tggcggccca ggccggagcg gcgcccgaga cgcccgcggt cgcgagcgcc tagcgcccgc
    27121 cgcggcgaag ccatcccccg cattcgaatc ccgaacgaag gaaggagtgt tccatgacga
    27181 tcaccgcctc gcacgtgaag gacctgcgcg agcggaccgg cgcgggcatg atggagtgca
    27241 agaaagccct ggccgaagcg aacggcgaca tcgagaaggc gatcgaggtg ctgcgcaagt
    27301 cgggcatggc ccgcgcgtcg gccaaggccg gccgcgaggc gcgccagggc accgtccacg
    27361 cctacatcca tcccggcgac cagctcgggg tgttgatcga agtgaactgt gagaccgact
    27421 tcgtggcgcg caccgacgac ttcaagcacc tcgtgcgcag cctcgcgatg caggtggcgg
    27481 ccacgaatgc ccaggcggtg gaccgcgccg cgctcgatcc cgcgttcatc gcgggggagc
    27541 gcgcgattct catggagcag gccaaggcca ccggcaagcc cgaagccgtg atccagaaga
    27601 tcgtggacgg gaagatcgag aagatgtacg aggaggtcgt gctcctcgaa cagccgacca
    27661 tccgggatcc cgacaggaag gtgaaggagc tgctggacca ggcgatcgcg aagctcggcg
    27721 agaacatcgt cgtgcggcgc ttcgccaagt tcaagatcgg ggaagcctga gcccggacat
    27781 ggccccctcg tctgaggtac cggcgcccca tcgagtcctg ctcaagctga gcggggagat
    27841 cctcgccggg gaggcgcgct tcggcatcga cgccgaggcc ttgggaaggg tggcggacga
    27901 gatcgtcgag ggcgcccgcg ccggaaccca ggtcggcatc gtgatcggcg gcggcaacat
    27961 gatccgcggc acggccaccg gcggcgccct ggaccgcacg acggccgaca gcatggggat
    28021 gctcggcacc gtcatcaacg cgctggcgct ccaggacgcg ctggaggagc gcgacctctc
    28081 gacgcgggtt ctctccgcga tcgagatgcg ctccctggcc gagccctaca tccgccgccg
    28141 tgccgtgcgc cacctcgaga aagggcgcgt cgtcatcctc gccgccggca cggggcaccc
    28201 ctacttcacc accgacaccg ccgccgtcct ccgcgcggtc gagatccgcg cggacgtgct
    28261 gctcaaggcc accaaggtgg acggcgtctt caccgatgat ccgatgcgga accccgacgc
    28321 ggtccgcttc gacacgctca gctacctgga agccatcgat cgccgcctga acgtgatgga
    28381 catgacggcg ttcacgatgg gcatggacaa ccagctcccg atcctcgtct tcaacgtgac
    28441 cgttccgggc aatctctccc gggcgctgcg cggggagatc gtggggacac gggtcgccgt
    28501 ggaggtctag ggatggggcc tgaaatcctg aagcagaccg aagagcggat gaagaaggcg
    28561 gtggagacga tccggcacga ggtcgccacc gttcgaacga accgcgcgac gaccgcgctg
    28621 ctcgacggca tcaaggtcga gtactacggc aacccgacgc cgctgcagca ggtcgcgagc
    28681 gtgagcgtcc cggagccccg catgctcctg atccagccgt gggagcgcgc gatcctcccg
    28741 gagatcgtga aagcgatcca gaagtcggac ctgggcttga acccggccga cgacggctcg
    28801 gtggtgcgcc tgacgctgcc gaccctgacc gaggagcggc gcaaggacct ggtgaagcac
    28861 ctgggcaagc tgatcgagga cgggcgcgtg cgcgtccgca cggtgcggcg cgaggtcaac
    28921 gacgagctga agaagctgga gcgggagcac aagatctccg aggacgacgc caagaagacg
    28981 cacgacgcgg tccagaagat ccacgaccgc tacatcgtca tgctggacga cctgtttcac
    29041 aagaagcagg ccgaggtcat ggaggtctga gcgacccggc gcaagaggat cgcatgcgcg
    29101 ccccatccaa agcccccacc ctggagcagg tgctccgccg acccgtcccc gagcacatcg
    29161 cgatcatcat ggatggcaac ggacggtggg ccaagcgtcg cggcctcccg cggctgatgg
    29221 gacaccgcgc cgggcgccgc tccgtgcgcg aggcggtcga gggatgcgtc gagctgggcg
    29281 tgcgcgtcct caccctctac accttctccc tcgagaactg ggagcgccgc gcgggcgagg
    29341 tgcgcgggct ctggcggatc ctggaacagg tgctccgcga agagcggaac gagctcaaga
    29401 agaacaacgt ccggctccgc acgatcggcc gggtggatct cctccccgag cggagccgcg
    29461 cgatcctggc cgagacgatg cagttcctcg agagctcgac cggactgacg ctggtgctgg
    29521 ccatctccta cggcggccgg gccgagatcg tggacgcggc ccggcgcatc gtggccgccg
    29581 accggaagca gcggatcccc ctgacgaaga tcgacgagag ctacttccag tcgcagctgg
    29641 acaccgacgg tctccccgat cccgacctcc tcatccgcac cagcggggag ctccgggtct
    29701 cgaacttcct cctctggcag ctcgcctacg ccgagttctg ggtgaccgac acgctctggc
    29761 ccgacttccg gcgcaagcac ctgtacgagg cggtccatga ctttcaaaac cgaagccggc
    29821 gcttcggccg cgcggattga tcccgcggcc cgagacggcg tgacgcggga cgccgagggg
    29881 gccgccgcgc ggccccgcgg cgatgccgcg ctgccgctcc ggatcgcgag cgcggtcgtc
    29941 ttcctgccgc tgttcgtgct cgcggcgcgc gtgggcgggc tggtctggct ggcgttcacg
    30001 ctgatcgtgg tcggcctggg gctccgcgag ttctatcgca tgatggactc gaaggggctg
    30061 gcaccccact ggaagtcggg gaccttcgcc gggctgctcc ttcccatcgg cacctacttg
    30121 cgcctgcaga ccgggcggac ggcggagtgg aacctgggcg cgctcttcac ggtgctcgcc
    30181 ggggcgatcc tgctcgcgga gctccggaga ggcgcgggga agcaggccgt ggggagcatc
    30241 gcggccacgc tcctcggcgt catctacctg ggctggttcg gaagccacat cgagctcctc
    30301 cgcgagctgc cgcgcgcctg gcacctgccg tacgcgctcg gcgcctcctt cgcgatcctc
    30361 cccttcttcc tggtttggat ctgcgacacg gcggcctacg ccgtcgggct ggcgttcggg
    30421 cggacgcggc tcatgcccga cgtgagcccg cagaagtcgg tggagggcgc ggtagcaggg
    30481 ctcctggccg cggtgggcgc cgcgttcgcg gcgcgcgcct ggttcgcgcc ctacctcacg
    30541 gccctcgacg cggccgtgct cgggaccctg gtcggcgtgc tggggcagct gggcgatctg
    30601 gtcgagtcgc tgttgaagcg cgacgccgac gccaaggaca cctcgcggat cattccggga
    30661 cacggcggcg tcctcgaccg cttcgacagc gttctcttcg ccgcgccggt caccgcctac
    30721 tacctcctct tccgggtgct cgaccgatga tccggatcgc cctgctcggc gccaccggct
    30781 cgatcggcgc cgccgcgcag gcgctcgcgc gcgcccaccc cgaccggatg cgcctggtgt
    30841 ccctgtcggc gcgcgcgaac gtcgaatcag tgctccgcgc cgccgaggaa ttcggtgtga
    30901 gccgggtggc cctgtccgat cccgaggccg cccgcgcgct tcgcgcgcgc ttccgcggcg
    30961 aggttctgga aggggacttg gccgcgacgc gcctggccga ggaccccgac gccgacgtcg
    31021 tcgtgaacgc gctcgtgggg agcgcgggac tctcgccgac ggtcgcggct ctccacgccg
    31081 gcaagcgcgt cgcgctcgcg aacaaggagt cggtcgtcct cgcgggcgag ctcttggcca
    31141 aggtcgcggc gcagggaggc ggcacgatcg tgccggtgga ctcggagcac ggcgggatcc
    31201 accaggcgct cgaagggtgc gcgctcgaca ccatccgccg cgtgacgctg accgcctccg
    31261 gcggcccctt cctgcgccgc gatcccgcca ccctggccga cgt
//
LOCUS       DASSLZ010000149         8299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_432664, whole genome shotgun sequence.
ACCESSION   DASSLZ010000149 DASSLZ010000000
VERSION     DASSLZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432664"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFM00_05715"
     CDS             complement(<1..322)
                     /locus_tag="VFM00_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497776.1"
                     /translation="MIRFRYTAAFIAAAVILLGSGSAEARQAQSAPGGGKPFAERVSE
                     DVGIARFALIRPGLARGGEPNQGGLHYLRDHGYRTVVSFLTDSAESSWVVNSGMRWIH
                     IPMRS"
     gene            492..734
                     /locus_tag="VFM00_05720"
     CDS             492..734
                     /locus_tag="VFM00_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497777.1"
                     /translation="MTPAGRRAWFNGRIRLGMLFLVLASLWRWLVHPGPQLSSNVVDA
                     AAGILYGISIGCMLTGLRLNALRGVGRGTDPDASAR"
     gene            778..1689
                     /locus_tag="VFM00_05725"
     CDS             778..1689
                     /locus_tag="VFM00_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019621480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7497778.1"
                     /translation="MTALFTLFALAGFAANSILCRVALGPRAIDPASFTAVRLASGAA
                     ALALLAAARRTRHQSSHAGSWTSAFLLFLYAMPFSFAYVSLGVATGALILFLSVQATM
                     LAGALIEGERIHWLEGLGILSALAGLVYLVAPGLSAPDPKGAALMAAAGAAWGIYTLR
                     GRRSQDAIGDTAANFVRSVPLAIAAGGLLWALGRAAGRAATPHVSTAGLLLATGSGVL
                     ASGAGYAAWFAALRGLRALQAAALQLTVPVIAAAGGILFLSERISIRLVVSAALILGG
                     VGMALGGRAARTRVVSRSPRSTKPLMG"
     gene            1819..3300
                     /locus_tag="VFM00_05730"
     CDS             1819..3300
                     /locus_tag="VFM00_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7497779.1"
                     /translation="MHANLASIMPQVIAVWCEEEGHQVHLTCYTGFEDLDTLIPPDID
                     LVFISAFSQAAQFAYALSNQLRARGAVTALGGPHARCYPEDARKYFDYVFGFTDKETV
                     RSALTDCAPHRPLGLLLDAKKHPATLPGVEERWKFIEPTLRKAPTFKIVPMIGSLGCP
                     YTCPFCIDSTVEYQPLGFEQIKQDLRFLRTKMKHPRVGWHDPNFGVRFNDYINTIEDA
                     VPPGSIDFLAESSLSLLSEPHLVRMKHNSFKAILPGIESWYSLGNKAGTGSKISGMDK
                     VKQVSAHVNTVLRYIPYVQTNFVLGLDMDEGDEPFELTKRFIDMTPGAFPGYSLLTAF
                     GKAAPLNLELQRAGRVSGFPFHFLNNNHAMNVQPKNYRWQEFYDRVIDLTKYSFSWRS
                     IGRRFRGTKEPIPKWMNVVRAISTEGFGRIRYHSRVRELLDADPSFLPYFTGQSDELP
                     QFYVERIKRDLGPLWPHLPEGSLFHDPNAYLKTHTAAIPMTVG"
     gene            3300..4424
                     /locus_tag="VFM00_05735"
     CDS             3300..4424
                     /locus_tag="VFM00_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET7497780.1"
                     /translation="MRFETRAVHAGGSPDRETGAATPPIHLSTTFEHGPAGEARGGHI
                     YIRESNPTQSRLEEALAAIEGGEGALVFASGVAAGAAYLQALEPGSHVLFHRDIYYAF
                     RTMAQEYLPRWGLESSFADLTDLAAARAGIRPNTRLLWAETPTNPLMQVLDLRALAAE
                     ASRAGARLLVDGTFATPALQRPLELGADVVLHSTTKYLGGHSDVLGGALVVRARDDRF
                     ARVQHNRHILGPIASPFASWMVLRGLRTLACRVERQSANALAIARALEGHPRLVAVHY
                     PGLLSNPGHEVAKRQMSAFGAMMALRVAGGRDGAVGVARRVRLFTNATSLGGVESLLE
                     HRVSVESPDTTTPDDLLRLSIGLEHPDDLIEDLRQALDGG"
     gene            4414..5103
                     /locus_tag="VFM00_05740"
     CDS             4414..5103
                     /locus_tag="VFM00_05740"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7497781.1"
                     /translation="MAAEPEAAPAPGLRQVLFPKRGFSATECLALANVTVAAVLFAVL
                     GSDYSPALSAWAFGRWADVHTHAAYGWFLPTLFLHAGVGHLTSNLIALLIAAGAVEFL
                     AGARWAVAAYFLTGMGAAWISFAGHPGPPLGIGASGAVMGLVGCTVSFIIRRRRDFNY
                     AQRWKVWRVYVPLFLLVFLPTLVHANVHAHVGGFVCGLLLGFFLPPHPRVAALAAFDS
                     LSDDEEPLPPE"
     gene            complement(5168..5305)
                     /locus_tag="VFM00_05745"
     CDS             complement(5168..5305)
                     /locus_tag="VFM00_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497782.1"
                     /translation="MNENLDLEVIEVEQGPKPGCTGSSSTSPRCTCPVAMTPPEEAEE
                     N"
     gene            complement(5436..6713)
                     /locus_tag="VFM00_05750"
     CDS             complement(5436..6713)
                     /locus_tag="VFM00_05750"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7497783.1"
                     /translation="MSGTHGAGAALRSRDFRLLWIAQGISQTGDSLTWLALILTINRL
                     TGSTTMIATLSVVLALPQLVLGLFAGVMADRWNRRRTMIGSDLLRAAIVLGFLLVRGP
                     EDLWLLYLLGLLHVVVSVFFSPARAALTPALVAPDALLSANSLLQTTQIVSGLAGTAL
                     AGVLIPMAKSGWPAFALDAVSFAASAACVAGMSASAGVPDAGSSPAKSGSRRGVMRDL
                     RDGLSLVLGTRALLAIVMAFGVASLGTGAVGVLFVPFLVNTLHLPAASVALAKGAQVG
                     GLLIGGAWVARSGMARSNAALVGGGLAGIGLATVFLGASPGLAAVLGSMLLLGLCATP
                     MQSATNALLQRRTPNAMLGRVEATVDTLLLAVMMGSMAWAGVLADRVGIRAVLYGAGI
                     LCLAGGAAGWALLTRVRVSGRSAAEGEPTIESG"
     gene            complement(6710..>8299)
                     /locus_tag="VFM00_05755"
     CDS             complement(6710..>8299)
                     /locus_tag="VFM00_05755"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HET7497784.1"
                     /translation="GQDVGPFYAKLVERGVLIGEVEIPWSERRPLRALAARCPGASWA
                     AGLETIEAEVDAMARLAPEAIPDRIDGLTRHLAALPRSRPLSEDDAIRCDAATALRVR
                     LPRTLLRDLERLLPAYARFYGGIYPEALTRETYAARFLKRYPPDTPIPALDFYHGLFE
                     PAPRKPLAAFTSAAAGSAPSPRRAEAGRAFERAKAFFTRRAREAANAGTEEVALCDED
                     WIEIAGDGAVPRFSCAALFQVEAASSDEVAASSARLCLNSIFPGAGLSVARLAHLHGG
                     AAEANPIVGALREGWARRARPGAELAEITFMHGGRTANAGLRPPLFRLEIELPGDRAT
                     EGHEAVPLADLTAAWDSAAGRFALRSVSRGIDILPVISSGINPEGFVQFLTMVGEQDL
                     QPLGLLPGFDDPEIRIWPRFRYGNVVLFRRRWVFAEEEMPSGATAEERFLAARRWVRA
                     HGLPEHVFVHSDREPKPFYVNLGSPAFLDLLRRSVDGAGRMHVTEMRPGPDALWVRDG
                     RGRYASEFLVHVDRLGEEGGA"
BASE COUNT         1252 a         2979 c         2818 g         1250 t
ORIGIN      
        1 cggagcgcat cggaatgtgg atccagcgca tgccggaatt caccacccag gagctctcgg
       61 cactgtcggt caggaagctc acgacggtgc gatatccgtg atcgcggagg tagtgcaggc
      121 ctccctgatt gggctccccg ccgcgtgcca gaccgggccg gatcaaggcg aagcgggcaa
      181 tccccacgtc ctcggaaaca cgttccgcga acggctttcc gccgcctgga gcgctctggg
      241 cctggcgcgc ctccgcgctc cccgacccaa gcaggatcac ggcggcggcg atgaaggcgg
      301 cagtgtatcg gaagcgaatc atggtcattc taccccggtt ccgggacggt ttctcctggc
      361 ccttagatga tcggcagcat ccggcggatg catcagctcc aatacctgcc cgcacgagcc
      421 gatggacagg atccatacgt gacctcatga tgaggagacg aaacgttcgt ctcccctctt
      481 tcggagccac gatgaccccc gccggccggc gagcctggtt caacggacgc atccgcctcg
      541 ggatgctctt cctggtcctc gcttcgctct ggcgctggct ggtccacccc ggaccccaac
      601 tttcttcgaa cgtcgtggac gcggccgccg gcatcctcta cgggatttcc atcggctgca
      661 tgctcacggg actgcggctg aacgcgctcc ggggcgtggg ccgggggacc gatcccgacg
      721 cgagcgcgcg ataggaatcg tcgcgcatag cccgggagca ccgaggcgat cgtctcggtg
      781 acggcgctct tcacgctgtt cgccctcgcg ggcttcgccg cgaactccat cctctgccgc
      841 gtcgcgctcg gcccgcgcgc gatcgacccg gcctcgttca ccgccgtgcg tctggcttcg
      901 ggagcggccg ctctggcgct gctcgcggca gcccggcgta cccggcacca gtcctcgcac
      961 gcgggctcct ggacatcggc gttcctcctc ttcctgtacg cgatgccctt ttccttcgcc
     1021 tacgtctcgc tcggcgtcgc gaccggcgcg ctcatcctct ttctctcggt acaggcgacg
     1081 atgctggcgg gggcgctgat cgagggagaa cggatccact ggctggaagg gctcggcatc
     1141 ctttcagcgc tggcgggcct ggtctacctg gtcgcgcccg ggctctcggc ccccgatccg
     1201 aaaggagccg cgctcatggc cgcggccggc gccgcctggg ggatctacac cctgcgcggc
     1261 cgtcgctcgc aggacgcgat cggagacacg gccgccaact tcgtccgctc ggtgcctctc
     1321 gcgatcgccg cggggggact cctctgggcg ctggggcgcg ccgcgggccg cgcggcgaca
     1381 ccgcatgtct cgaccgcggg cctccttctt gcgaccggct ccggcgtgct cgcttcgggc
     1441 gcgggctacg ccgcctggtt cgcggcgctt cggggcctgc gcgcgcttca ggccgcggcg
     1501 ctccagctga cggtgcccgt gatcgccgcc gcgggcggga tcctctttct ctcggagcgg
     1561 atctcgatac gcctcgtggt ttcggcggcg ctcatcctcg ggggggtcgg aatggcattg
     1621 ggtgggcgcg cggcacgaac tcgcgtagtc tcccgtagtc cgcggtcaac aaaaccactt
     1681 atgggctagc cccgggcgcc gcttctccgg tagcgttttc atgggcattc aacgaaggga
     1741 gtagtcatcc gcaaactacg catcgctgta ctcgatatcg tcgcccgggc accgacccgg
     1801 gccctctacg cgcgcatcat gcacgccaac ctggccagca tcatgcccca ggtcatcgcg
     1861 gtctggtgcg aggaggaggg ccatcaggtc cacctcacct gctacaccgg cttcgaggac
     1921 ctcgacaccc tgatcccgcc cgacatcgac ctggtcttca tcagcgcctt ctcgcaggcg
     1981 gcgcagttcg cctatgcgtt gagcaaccag cttcgcgcgc gcggcgcggt gaccgccctg
     2041 ggcgggcccc atgcgcgctg ctatcccgaa gacgcccgga agtacttcga ctacgtcttc
     2101 ggcttcaccg acaaggagac ggtccgttcg gcgctcaccg actgcgcgcc gcaccggccg
     2161 ctcggccttc tgctcgacgc gaagaagcac ccggcgacgc ttccgggcgt cgaagagcgg
     2221 tggaagttca tcgagccgac gctccggaag gcgccgacct tcaagatcgt tccgatgatc
     2281 ggcagcctgg gctgccccta cacctgcccc ttctgcatcg attccacggt ggagtatcag
     2341 ccgctcggct tcgagcagat caagcaggac ctgcgcttcc tccggaccaa gatgaagcat
     2401 ccgcgcgtgg ggtggcacga tcccaacttc ggcgtgcgct tcaacgacta catcaacacg
     2461 atcgaggacg cggtccctcc gggcagcatc gacttcctcg cggagagcag cctctcgctc
     2521 ctctcggagc cgcacctggt ccgcatgaag cacaacagct tcaaggcgat cctgccgggc
     2581 atcgaatcgt ggtattcgct ggggaacaag gcgggcaccg gcagcaagat cagcggcatg
     2641 gacaaggtga agcaggtgtc ggcccacgtg aatacggtgc tgcgctacat cccctacgtg
     2701 cagaccaatt tcgtcctcgg cctcgacatg gacgaggggg acgagccgtt cgagctgacg
     2761 aagcggttca tcgacatgac gccgggcgcg tttccgggct actccctgct caccgcgttc
     2821 ggcaaggcgg ccccgctcaa tctggaattg cagcgcgccg ggcgcgtttc cggtttcccc
     2881 ttccacttcc tgaacaacaa ccacgcgatg aacgtccagc cgaagaacta ccggtggcag
     2941 gagttctatg accgcgtgat cgacctcacc aagtacagct tctcctggcg ctcgatcggc
     3001 aggcgcttcc ggggcacgaa ggagccgatt cccaagtgga tgaacgtcgt gcgcgccatc
     3061 tccacggagg gattcggccg gatccgctat cattcgcggg tgcgcgagct cctggatgcc
     3121 gacccctcgt tcctccccta tttcacgggg cagagcgacg agctgccgca gttctacgtc
     3181 gagcgcatca agcgggacct cgggccgctc tggccgcatc ttccggaggg ctcgctgttc
     3241 cacgacccca acgcctacct caagacgcac accgcggcca ttcccatgac cgtgggctga
     3301 tgcgcttcga gacgagagcg gttcacgcgg gagggtcgcc cgaccgggaa acgggggcgg
     3361 ccaccccgcc gatccatctc tccacgacgt tcgagcacgg gcccgccggc gaggcacgcg
     3421 gcggccacat ctatatccgc gagtcgaatc cgacgcagag ccgcctggaa gaggcgctcg
     3481 cggccatcga gggcggggaa ggggcgctgg tcttcgcttc cggcgtcgcg gcgggggcgg
     3541 cctatctcca ggcgctcgag ccgggctcgc acgtcctctt ccaccgcgac atctattacg
     3601 cgttccgcac catggcgcag gagtacctgc cgcggtgggg gctggaatcg tcgttcgcgg
     3661 acctgaccga tctcgccgcc gcaagagccg ggatccggcc caatacgcgc ctcctctggg
     3721 ccgagacgcc caccaatccc ctgatgcagg tgctcgacct gcgcgcgctg gccgcggagg
     3781 cgtcgcgggc cggggcgcgc ctgctcgtgg acggcacctt cgccacgccg gcgctccagc
     3841 ggccgctcga gctgggcgcg gacgtcgtgc tccactcgac gacgaaatac ctgggcggac
     3901 acagcgacgt cctggggggg gcgctcgtcg ttcgcgcccg cgacgaccgg ttcgcccgcg
     3961 ttcagcacaa ccgccacatc ctgggcccga tcgcctcgcc cttcgcctcc tggatggtgc
     4021 tgcgcggcct gcgcacgctc gcctgccggg tggagcggca atcggcgaac gcgctcgcga
     4081 tcgcgcgcgc gctggagggc cacccgcgac tggtcgcggt gcactatccg ggcctgctct
     4141 ccaaccccgg gcacgaggtg gcgaagcgtc agatgagcgc gttcggcgcg atgatggcgc
     4201 tgcgcgtggc gggagggcgc gacggggccg tcggggtggc gcggcgcgtg cgcctgttca
     4261 cgaacgcgac cagcctgggc ggcgtcgaga gcctcctcga gcaccgcgtt tcggtggaga
     4321 gcccggacac gacgaccccc gacgacctcc tccggctctc gatcggtctc gagcacccgg
     4381 acgacctgat cgaggatctg cgccaggcgc tggatggcgg ctgagcccga agccgctccg
     4441 gctccgggtc tccgccaggt tctgtttccc aagcgcggct tctccgcgac ggagtgcctc
     4501 gcgctggcga atgtcacggt ggcggccgtc ctcttcgctg ttctgggatc cgactacagc
     4561 cccgcgttga gcgcctgggc atttggacgc tgggccgacg tgcacacgca cgcggcctac
     4621 ggctggttcc ttccgaccct cttcctgcat gcgggcgtgg gacacctcac cagcaacctg
     4681 atcgccctcc tgatcgcggc gggcgcggtg gaattcctcg cgggagcgcg atgggcggtc
     4741 gcggcctact tcctgacggg gatgggcgcg gcgtggatct cgttcgcggg gcaccccgga
     4801 ccgccgctgg gcatcggggc ctcgggcgcg gtcatggggc tggtgggctg caccgtcagc
     4861 ttcatcatcc gcagacggcg tgacttcaac tatgcgcagc ggtggaaggt ctggcgcgtc
     4921 tacgttccac tcttcctgct cgtcttcctt cccactctgg tccacgccaa cgtgcacgcg
     4981 cacgtgggcg gattcgtgtg cggtctcttg ctcggcttct tcctgccgcc gcatccccgc
     5041 gtcgccgccc tcgcggcgtt cgactccttg agcgacgacg aggagccgct cccgccggaa
     5101 tagaaggaag ggcggagcgg ctggagcgcc gcaccgccct tcgggacagc gggggatgaa
     5161 accccgatca gttctcctcg gcctcttccg gcggcgtcat cgccaccggg caggtgcagc
     5221 gcggcgaggt gctggaggac ccggtgcagc ccggcttggg accctgttcc acttcgatga
     5281 cttccagatc caggttctcg ttcatggtgg acctcctgcg tggcacggat gagagaagcg
     5341 ggcttcggcg cccgcggacc cgcggtgctc gcgcaccgcg aacggacgtt cgccccccgc
     5401 gaaccgcgat actacgcaca tcgcgcggcg acgatctagc ccgattcgat cgtcggttcg
     5461 ccctccgcgg cggaccgccc cgaaacacgc acccgcgtca gcagggccca cccggccgcg
     5521 cctccggcca ggcagagaat gcccgccccg tagaggaccg cacggatccc cacgcggtcc
     5581 gccagaacgc ccgcccacgc catcgacccc atcatgaccg cgagaagcag cgtgtccacg
     5641 gtggcttcca cccgccccag catggcgttc ggcgtgcgtc gctggagcag cgcgttggtg
     5701 gccgattgca tcggcgtggc gcacaggccc agcaggagca tcgacccgag cacggcggcc
     5761 aggcccggcg aggccccgag gaaaaccgtg gcgagaccga ttcccgccag acccccgccc
     5821 acgagggccg cgttcgagcg ggccattccc gatcgcgcca cccaggcgcc tccgatcagg
     5881 agcccgccga cctgggcgcc cttggccagc gcgaccgagg cggccggaag gtggagcgtg
     5941 ttcacgagga agggaacgaa caggacgccg accgcgccgg tgccgaggga cgcgacaccg
     6001 aacgccatca cgatggcgag gagcgcgcgc gtgccgagca ccagggagag cccgtcacgg
     6061 aggtcccgca tcaccccgcg ccgcgagccg gacttcgcgg gagagctgcc ggcatccggc
     6121 acccccgccg aggcgctcat tcccgccacg cacgccgccg aggccgcgaa gctcacggcg
     6181 tcgagcgcga aggccggcca gccgctcttc gccatcggga tgagcactcc ggccagcgcc
     6241 gtcccggcca ggccgctcac gatctgcgtg gtctgaagga gcgagttggc cgagagcagc
     6301 gcgtcgggcg cgaccagggc cggggtcagg gcggcccggg ccggactgaa aaagacgctc
     6361 acgacgacgt ggaggagtcc cagcaggtaa aggagccaga ggtcctcggg gcctcgaacc
     6421 agcaggaagc ccagcacgat cgcggcgcgg aggaggtcgg agccgatcat ggtgcgccgc
     6481 cggttccacc ggtcggccat gacgccggcg aagaggccga gcaccagctg cggcagggcc
     6541 agcaccaccg acagcgtggc gatcatcgtg gtcgagccgg tcagccggtt gatggtcagg
     6601 atgagggcga gccaggtgag gctgtctccg gtctgagaga tcccctgcgc gatccagagc
     6661 agccggaagt cccggcttcg gagcgccgct ccggcgccgt gcgttccgct cacgcgccgc
     6721 cctcttcgcc gagacggtcc acgtgcacga ggaactccga agcgtaacgg cccctcccgt
     6781 ccctgaccca gagggcgtcg ggaccggggc gcatctcggt cacgtgcatc cgtcccgcgc
     6841 catccaccga ccggcgcagg aggtcgagaa acgccggcga gcccaggttg acgtagaacg
     6901 gcttgggctc ccggtcgctg tggacgaaga catgttccgg caggccgtgg gcgcggaccc
     6961 accggcgcgc ggcgaggaat cgctcctccg ccgtcgcccc cgagggcatc tcctcctccg
     7021 cgaagaccca cctccgccgg aagagcacca cgttgccgta tcgaaaccgg ggccatatcc
     7081 ggatctccgg atcgtcgaag ccgggcaaga gtccgagcgg ctgcagatcc tgctcgccca
     7141 ccatcgtgag gaactggacg aagccctcgg gattgatgcc gctcgagatg acaggcagga
     7201 tgtcgatccc gcgcgagacc gagcgcagcg cgaaccgccc ggccgcggaa tcccacgcgg
     7261 cggtgaggtc cgcgagcgga accgcctcgt gtccctcggt cgcgcggtcg cccggaagct
     7321 cgatctccag gcgaaagagc ggggggcgga gccccgcgtt cgccgtgcgc ccgccgtgca
     7381 tgaacgtgat ctcggcgagc tccgcgccag gccgggcgcg gcgcgcccac ccctcgcgca
     7441 gcgcgccgac gatcggattc gcttccgccg cgccgccgtg caggtgggca agccgggcga
     7501 ccgagagccc cgctccggga aagatcgagt tcaggcagag gcgcgcggag gaagcggcga
     7561 cctcgtcgga ggacgccgcc tcgacctgga agagcgcggc gcaggagaat cggggcaccg
     7621 ctccgtcgcc ggcgatctcg atccagtcct cgtcgcacag cgccacttcc tcggtgcccg
     7681 cgttcgcggc ctcccgggcg cggcgcgtga agaacgcctt cgcccgctcg aaggcgcggc
     7741 ccgcttccgc gcggcgcggg gaaggggcag agcccgccgc ggcggaggtg aacgccgcaa
     7801 ggggcttccg cggcgccggc tcgaaaagac cgtgatagaa gtcgagcgcc gggatcggcg
     7861 tgtccggggg gtagcgcttc aggaaccggg ccgcgtacgt ctcgcgggtc agcgcttcgg
     7921 ggtagatccc tccatagaag cgggcatacg cgggcaacag ccgttccagg tcgcggagca
     7981 gagttcgggg tagccggacg cgaagcgccg tcgccgcgtc gcagcggatc gcgtcgtcct
     8041 cggagagcgg ccgcgagcga gggagcgcgg cgaggtgcct ggtgaggccg tcgatccggt
     8101 ccgggatcgc ttccggcgcc aagcgcgcca tcgcgtccac ctcggcctcg atcgtttcga
     8161 ggccggcggc ccacgaagcg ccggggcatc gcgccgcgag cgcgcgaagc ggcctccgtt
     8221 cgctccaggg gatctccacc tcgccgatca ggacgccgcg ctccacgagc ttggcgtaga
     8281 aaggcccgac gtcctggcc
//
LOCUS       DASSLZ010000150        17632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1445920, whole genome shotgun sequence.
ACCESSION   DASSLZ010000150 DASSLZ010000000
VERSION     DASSLZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 17632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17632
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445920"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VFM00_05760"
     CDS             complement(<1..1462)
                     /locus_tag="VFM00_05760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET7497785.1"
                     /translation="MKGPPGLEAWTVAFIGSSLAAAVIVPIAARASVAPAGAALIACF
                     AALPWAARRVPDSLRGSLRRRPLVALLWLLLSLVAIAQMGRLSAFMADSSRLWGSTFP
                     DPVVSGHQCMSAYVHAADLARRGEENIYDPRWYPAFTAPVNTTPGFPSPVAGLSRWID
                     DPYEYPSPFLLLPRAALAATNSFDAIRAGWFALQTLALMATVLLLAVWVGGRYGLTAG
                     LLLPLLFASIPTMFDLQFGQFHMMAVALTVAAMIAFEERRPVLGGALLAFAILGKVFP
                     AMFLLYLALRRQWRAAGWTVAFLAAFTLLSIPVVGWKPLLAFFTFQVPRIVTGEAFSF
                     VSRPGVPLFFLSRNFSITSVVPKLGVLGVPGMTRGVSLALTWLFTLAILAFNQRAARG
                     FRSRVGEARTWLALLTIASLRAPLAPSSYVTVTPLWLLTYLAGGIRGRVPLVIAFALT
                     WALIVGAPPLPDRADLVVGLVGQALAFGIAIATLVRP"
     gene            complement(1462..1953)
                     /locus_tag="VFM00_05765"
     CDS             complement(1462..1953)
                     /locus_tag="VFM00_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7497786.1"
                     /translation="MSTAYAPAFRRRECVRALILTPEPALLLIHLVVPDRKLWIAPGG
                     GIDPGESHQEALRRELDEEVGRGNFEIGPQVWVRRGRFRWAGQWFDEREHFYLIRSDR
                     FEPDASRNPVAFERDAMAEMRWWPVAELPAESKQFAPMRMGSLVADLIQGGAPSEPVE
                     TGF"
     gene            complement(1950..2561)
                     /locus_tag="VFM00_05770"
     CDS             complement(1950..2561)
                     /locus_tag="VFM00_05770"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HET7497787.1"
                     /translation="MPVRTVRLRMHGEIKLKTWRTFGADETLTWNQEMMWRARTSMNG
                     LPISGYDRVAPGIGEQRWKLLGLVPVMTASGHDIARSGAGRMAGESCWIPSVLRGRDV
                     KWSSQDPSRARAAFTVLGERADLALTLGSDGRLESVMVARWGNPDGGLFRYETFGGVM
                     ESEAAFQGYTIPTRMRMGWYFGSDRFEREGEFIRIVIDDAHYE"
     gene            complement(2561..3055)
                     /locus_tag="VFM00_05775"
     CDS             complement(2561..3055)
                     /locus_tag="VFM00_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7497788.1"
                     /translation="MRRPTMADAPGVFEYASDPEVTRYLGFTTHRALDTVEEFLRGLL
                     QAMEQGGRYGWALTPSGSDRLIGMIEIRVQPPRAELGYVLGNPYWGQGYMVEAVRPIV
                     EWALGQGPIHRVWAFCDADNRGSARVLEKVGMAREGTLRKWAMSPNLSDAPRDCHCYS
                     IVRD"
     gene            complement(3094..3861)
                     /locus_tag="VFM00_05780"
     CDS             complement(3094..3861)
                     /locus_tag="VFM00_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7497789.1"
                     /translation="MTELPEHALHNRAYWDQKASDYVASGERHWAASEPTWGIWNLPE
                     REIGMLPCDLSQRDAIELGCGTAYVSSWLARRGARVVGIDNSEAQLATARRLQREHGL
                     SFPLVHGNAESVPYPDASFDFAISEYGACLWADPERWVPEAARLLRPGGRLHFLTNAF
                     LLMLCMPDDETQAAGERLLRPAFGMGRIAWPGDSGVEFHKSHGEWFRILRGAGFEVED
                     LVEVRPPEGATTRYPFVTAEWARQWPSEEVWKARKRP"
     gene            complement(3858..4436)
                     /locus_tag="VFM00_05785"
     CDS             complement(3858..4436)
                     /locus_tag="VFM00_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497790.1"
                     /translation="MAVRTPVLPLERLLRERLIVEEDPGTADLIAGLRPARARGYLTK
                     GEFERVCRWKSPRSIGLVRGNNHHRVRKATTTVLSTVDERKRFDALLALHGVSVPTAS
                     AILTMLEPKRYGVIDIRVWQLLHAMGAVEGNARGAGFTFDQWLRFLRVIRDLAAQLGV
                     SARTVERTLFAAHREHQIGTLYRKAPTRSVSA"
     gene            complement(4426..4839)
                     /locus_tag="VFM00_05790"
     CDS             complement(4426..4839)
                     /locus_tag="VFM00_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HET7497791.1"
                     /translation="MPEPSPYDYAIVRVVPKVEREEFINAGAIVSCPASDFLEARIEL
                     DEGRLLALDPGADLATIRAHLATLPAICAGGPDAGPIGALSKRERFRWLTAPRSTVIQ
                     TSPVHTGACTDPAALLEHLLATMVRAPRKEGERGR"
     gene            complement(4826..5590)
                     /locus_tag="VFM00_05795"
     CDS             complement(4826..5590)
                     /locus_tag="VFM00_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HET7497792.1"
                     /translation="MRTVAATRYVTPLREGGSLPAIIEADDDGMYVLKFRGAGHGAKA
                     LIAELVCGEIARAAGLPVPEIVFVELDSDLARTEPDPEIQDLIRASDGLNLALDYLPG
                     SIMFDPVAERPDPGFASLVVWFDAFVTNIDRTPRNTNLLVWHRRTWLIDHGSALYFHH
                     TWEHWLERSRDPFPMIRDHVLLPSASRMEEADAALEKRITPDELRRIVSLIPDGWLVD
                     APFATASEHREAYTKYLARRLETPRAFLEEAIRARA"
     gene            complement(5591..5944)
                     /locus_tag="VFM00_05800"
     CDS             complement(5591..5944)
                     /locus_tag="VFM00_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7497793.1"
                     /translation="MSGAVSLAAKLATFAEHWQPRTVTTFNGHDVMVAKVKGEFVWHK
                     HDDTDDLFLVLQGTLTLELRDGAVTLGPGELFVVPRGVEHRPVAREEVHLLLLERTGT
                     PNTGDPATAATRRVI"
     gene            complement(5941..6411)
                     /locus_tag="VFM00_05805"
     CDS             complement(5941..6411)
                     /locus_tag="VFM00_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7497794.1"
                     /translation="MAKTYRKGPVGAMMDEYERASGELIRILEGISDEEFDRVRDTVT
                     QDEDCRSIQTVMRHVVRAGYGYANYMRTHWKKEPVVRWDEPVRRADTPAEMRKMLAYM
                     VETLEGHWDMTDQECTEMKMTVRWGPIFDFEQLFEHAIVHILRHRRQIERFLGR"
     gene            6606..7877
                     /locus_tag="VFM00_05810"
     CDS             6606..7877
                     /locus_tag="VFM00_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497795.1"
                     /translation="MGRKHRSFRISLPPVVGRVAKSALKSKLLMVLVGLFVLGFIAGK
                     QSEPDPTGAPFQELFENLSVVSYRDSPSDSASHFMVELSARGKVFREYDVDEQRFVAP
                     AHGRDYGRMIGATRYEPLIVRGHVDRGFWLELSNSKSPEILPDQFQEMYQTTLDYVKP
                     VSVIASIAGTLSGYSVGYRAGTWGTSLANPAVQKRVLATSGVGRMITREAWRRVLLEP
                     VVMGHESDPERFAAARETQRIYTNFFRLALADSNDFIHTEARRLDAAGYTREAIAMLE
                     FTYSALRAADDTVHLESADFRAVEEWASLIAHRGHWAPTSIPASGEARVRYLGTLAYY
                     GLTPVADDAQRVWVGPRVLVKDGDEEGFVADDIPSIRAACPLTWQDWLKHDTTGLSSN
                     EWTAQWMKESKQLAQMVSFGKQVVRQIQPAR"
     gene            7928..8674
                     /gene="lexA"
                     /locus_tag="VFM00_05815"
     CDS             7928..8674
                     /gene="lexA"
                     /locus_tag="VFM00_05815"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET7497796.1"
                     /translation="MRTKPNPSGARGKRPPTRTPSPEAILAYIEESLRDTGRPPTIRE
                     IGAAFGIASTNGVRYHLDKLEREGRIRRDRFTSRGIEVQLAGGRGATHMDTLPSMSPA
                     FRRANASSPGTDARARELLEIPLLGHVAAGAPLLAEEHIEDTLTLDPAMVRPGGKHFA
                     LRVRGDSMKNAGILDGDVVIVRHDTPVKSGEIAVVLIGDEATVKRFFPRRDKLLLFPE
                     NEDYEPIEVRPADPDVRVAGKVVGVFRRLG"
     gene            8674..8970
                     /locus_tag="VFM00_05820"
     CDS             8674..8970
                     /locus_tag="VFM00_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HET7497797.1"
                     /translation="MLHARSFPSERDGYFMIESIHDPIEVITMFSGGTMKPIRFRWKS
                     KTVRIAKVTGDWVRHEGENKIHYFALLADNADYYEIRYDAREMTWQLTRVWMEG"
     gene            complement(8945..9439)
                     /locus_tag="VFM00_05825"
     CDS             complement(8945..9439)
                     /locus_tag="VFM00_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497798.1"
                     /translation="MKHALTLTEQTRRVAPLLAERLRTLHPELHPSVDSAQVHMSVHL
                     PVPGRPGHELRADLYEEGATIAYDDGLPPGPAEKILVWGTASDGAILYAMIGAMEEVF
                     ANRVVLVREPLSGWVRFLRRQDCDSVLGFVPLEDFRRWPERRRKRLLRSWTWSGATPP
                     STPA"
     gene            complement(9436..10185)
                     /locus_tag="VFM00_05830"
     CDS             complement(9436..10185)
                     /locus_tag="VFM00_05830"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7497799.1"
                     /translation="MTQPLPQAILLDLDDTILDDSGNVSHCWLEACRAHASELGAIDP
                     SALHAVIERTRAWFWSDPDRHREGRLDLDAARREVVRASLAEIGLETQSLAEKIAEHY
                     RVQRHMGLEPLEDAIETVRWLREKGCRLALLTNGAAAAQRSKVARFQLAALFDLILIE
                     GELGYGKPDPRVYKIALDELEASPRHTWMAGDNLEWDVAAPQRLGIYGIWVDRRGSGI
                     PPGNGTRPDRIVRSLSELRLAPAQVDESATP"
     gene            complement(10196..11002)
                     /locus_tag="VFM00_05835"
     CDS             complement(10196..11002)
                     /locus_tag="VFM00_05835"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7497800.1"
                     /translation="MSPDVWAAGKYYEPFVGRWSRLAARSFLDWLDLPAGRHWLDVGC
                     GPGALAQTILDRVRPASILGVDPSAPFIEYARTHVRRDGLAAEAVSFRVGDARALPVE
                     DASRDAVVSGLVLNFVPQPERAVAEMARATCPGGVAAAYVWDYAGRMELLRYFWDAAV
                     ALDPDAAALDEGPRFPICRLEPLEALFTGAGLGSVATTTIDVPTVFRDFDDYWNPFLG
                     GQGPAPAYAMTLDEARRGRLRERIRSRLPFEKDGSIHLIARAWAVRGIKS"
     gene            11170..12441
                     /gene="dinB"
                     /locus_tag="VFM00_05840"
     CDS             11170..12441
                     /gene="dinB"
                     /locus_tag="VFM00_05840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET7497801.1"
                     /translation="MRIVAHVDMDAFFASVEEKLNPRLAGKPLVVGGSRDSRGVVSAA
                     NYAARPYGIHAGMPIAEAVRLCPEAAFIEGDPKKYVHISLQILDTLKDFTPIVEPFSI
                     DEAFLDLTAVEQLRPRKDDGDPETFLNAAIPLAQAIQRAVHRRVGLTASIGIAPNKYV
                     AKMGSGLRKPNGLTVLSVRRFREIFESKPVFELWGIGEKTAASLATLGIRTAGQLARF
                     PREFLTYHFGLSGDHMQDAARGDDESQVVPYFEGVPVKSMGHEYTLPEDVSDRERIRA
                     HLLRLSDQVGRRLRVDGYLGRVIAVKFRNARFKTTIRQRALAEVTDDENVIYGTACIL
                     LDDNWDGKPLRLVGVSVSDLVSAQGFHQHDLFSTDERRRRMTEAADSLRNRFGDAVLV
                     RAGALDQGDPQRLSREGASRSLVRVLADERR"
     gene            12438..13430
                     /locus_tag="VFM00_05845"
     CDS             12438..13430
                     /locus_tag="VFM00_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7497802.1"
                     /translation="MKDEPKIVLGTSGWSFKDWVGPFYPPGTERARMLDYYVREFPAV
                     EVNSTYYRIPHWRTFEAIAKKTPPGFEIVVKAHHDMTHLRSRESDLYRDFDRSVRPLR
                     EAGKLTGILAQFPYGFRRTRENEEFLHALPDLLGREVPLFVEFRHRSWVDEAAFRELQ
                     QEGLGFVSVDEPDLPGLVPPIARRTGPIGYVRFHGRNKEKWWGVESRDRGAPDRPGAK
                     RDAAKSAVPDARDKARTRVRAGADLGPLFSGTPDHPAEAPPLPAPPASEPRTSSTRYD
                     WSYTEDELREWVDKIREMTQQAKKTFVFFNNCHVGQAATSAKLMRKLLEGEGML"
     gene            13456..15963
                     /locus_tag="VFM00_05850"
     CDS             13456..15963
                     /locus_tag="VFM00_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7497803.1"
                     /translation="MTGGGVAGGSDLVHGPGLFDAERGERGRRSGAPETLDEYEALLH
                     ALTSRDRRFVHGPDGLRRRQNELVHVERLPARPAVYGDLAQPLPQALQAVLHTAGIER
                     LYTHQARAVELARSGANVAVATGTASGKSLAYHLPILERLLLEPGATALYLFPTKALA
                     QDQLRGLLRFGAASPTLGRSLATGTYDGDTPGEARRKLRERANAILTNPDMLHQGILP
                     YHARWSRIFSNLRYVVVDEMHTYRGVFGSHVANVLRRLKRVARHYGADPRFLLSSATI
                     RNPGELAESLVGEPVHVVDEDGAPRGPKLFAFWNPKHAGPEQAGARTSTSIEAERILV
                     SLIRRQVASIAFTRSRVAAELIYRYAREQLEERGRRRADKTDGGPNSLADRLSPYRGG
                     YLPEERRAIERRLFDGDLLGVISTNALELGIDVGSLDAAVLVGFPNTIASTWQQAGRA
                     GRSQAPSLAVVVAYDDPIDQYLMRHPEYFFAQSPESAVVDPSNPYVLASHLACAAHEL
                     PLSREDEAWFGPRTEALGRLLEEEGTVKSLDGLRYWSSSEYPAGRVNLRTISDDTYTI
                     LERKPGVPDAEARTLATVDAISAPELIYPEAIYLHEGDTWFVRELDLQQKVAYVERQE
                     VDYYTQPVLDTRLRLTETRETKRCGECEVGLGTANVSWATVAFKKIRFRSLDAIGYHP
                     LDLPRLNLDTVALWIRPDESARAALRVKGMNPAEGMSGLRNLLITLLPLHAMCDRPDL
                     GGVLDSANLGEQAIFLYDRYPGGLGYAERGYAIVDDLLRSTLRMIEDCPCEIGCPSCV
                     GLPVLRPAQQQDYDLHGGWPIPNKAATETLLRRLLGA"
     gene            15997..17025
                     /locus_tag="VFM00_05855"
     CDS             15997..17025
                     /locus_tag="VFM00_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET7497804.1"
                     /translation="MLSKTLRDRIGGLLRQGALDVPAPLSNGGMNGHAVGASDKAGAA
                     PERFVPEPRRHFVATGIEEILPGGVIDTAQGTVFAHERLYTDLGERPVPLLRRLAELC
                     AYPGCAPEPSRAGPHWVEIPGVDCPYPALPERARARRRRPRDLAEATGLHDPPEQGLF
                     RRFGYRRVLYLDIETCGLSAAPVFLVGLCMVGERNLILRQLFARDYAEERALLVELKR
                     LAGEHDFLVTFNGKAFDVPFLRDRAIHHRLEPPITMPHLDLLWMARRRWKDLLPDCKL
                     KTIEWRVLRRRRAGDVGGSEIPGLYHDYVKRGEAHRLLPVFHHNLLDVVAMVELTPVI
                     FDPDVGTY"
     gene            complement(17042..17614)
                     /locus_tag="VFM00_05860"
     CDS             complement(17042..17614)
                     /locus_tag="VFM00_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7497805.1"
                     /translation="MTEPIQLQTERLLLIASTPAIARAEATDRGELARLLGAEIHEAW
                     PPGENDQEGRDFLCRQLEEHPEAVGFLGWSWLVRAQVAPPERRPLLIGGGGFKGPPDA
                     EGTIEIGYSIVEEYYGRGLATEAVGATLAWAFGDPRVRRVVAETLPELRPSQRVLEKS
                     GFVRTDEAPAGPGSLRYELTRERWSARNSA"
BASE COUNT         2856 a         6133 c         6026 g         2617 t
ORIGIN      
        1 cggggcgaac cagcgtcgcg atcgcgatgc cgaacgcgag cgcctgaccg accagcccca
       61 ccacgagatc ggcccgatcc ggaagcgggg gcgctcccac gatcagcgcc catgtgagcg
      121 cgaaggcgat caccagcggt acgcggccgc gaatgcctcc cgccaggtac gtcagcagcc
      181 agaggggcgt gacggtgacg taggacgatg gcgcgagcgg cgcccgaagc gacgcgatcg
      241 tcagcagcgc gagccaggtg cgcgcctcgc ccacgcggct ccggaatccg cgagcggctc
      301 tctgattgaa cgcgaggatc gcgagggtga agagccaggt caaggccagg ctcacccccc
      361 gggtcatccc cggcacgccg agtaccccga gcttcgggac gacgctcgtg atcgagaagt
      421 tccgcgacag gaagaagagc ggcacgcccg gccggctcac gaaggagaac gcctcgccgg
      481 tgacgatgcg cggcacctgg aaggtgaaga aggccaggag cggcttccat ccgacgaccg
      541 ggatcgagag gagcgtgaac gcggcgagga aggcgacggt ccatccggcc gcgcgccact
      601 gacggcgcag cgccaggtag agaagaaaca tcgcggggaa gaccttcccg aggatcgcga
      661 acgcgagcag ggctccgccg agaaccgggc gccgctcttc gaacgcgatc atcgccgcca
      721 cggtgagcgc gactgccatc atgtggaact gcccgaattg aagatcgaac atcgtcggga
      781 tcgaggcgaa gagaaggggc aggagcaggc ccgccgtcag tccatagcgc ccgccgaccc
      841 acacggcgag gagcagaacg gtcgccatca gcgccagcgt ctggagcgcg aaccatccgg
      901 cgcggatcgc gtcgaaggag ttcgtggccg ccagcgccgc gcgcggcagg agcaggaagg
      961 gcgaagggta ttcgtacggg tcgtcgatcc agcggctcag tcccgcgacc ggagagggaa
     1021 atcccggcgt ggtgttcacg ggcgccgtga aggcgggata ccaccgcgga tcgtagatgt
     1081 tctcctcgcc gcgccgggcg aggtcggccg cgtgcacgta ggcggacatg cactggtggc
     1141 ccgagacgac cgggtcgggg aacgtggatc cccacagccg cgacgaatcg gccatgaacg
     1201 ccgagaggcg ccccatctgc gcgatcgcca cgagcgagag gagaagccag agcagagcca
     1261 cgagcgggcg ccgccgaagc gatccgcgca gcgagtcggg aacgcggcgg gcggcccagg
     1321 gaagggccgc gaagcaggcg atgagggccg cgccggccgg cgcgaccgac gcccgcgccg
     1381 cgatcgggac gatgacggcg gcggccagcg aggagccgat gaaggcgacc gtccacgcct
     1441 cgaggcccgg gggtcccttc actagaaccc cgtttcgacc ggttccgacg gcgcccctcc
     1501 ttggatcagg tccgcgacca gggatcccat tcgcatcggg gcgaattgct tcgactcggc
     1561 cggcagctcc gcgaccggcc accatcgcat ctcggccatc gcgtcccgct cgaaggccac
     1621 cggattgcgc gaggcatcgg gctcgaaccg atcgctccgg atcaggtaga agtgctcgcg
     1681 ctcatcgaac cactggccgg cccagcggaa ccggccgcgg cggacccaga cctgggggcc
     1741 gatctcgaag ttccctcgtc ccacctcttc gtccagctcc cggcgcaacg cttcctgatg
     1801 cgactcgccg ggatcgatgc cgcctccggg ggcgatccag agtttccgat ccgggacgac
     1861 caggtggatc aggaggagcg ccggttcggg cgtgaggatc agcgcgcgca cgcactcgcg
     1921 acggcggaag gccggagcat aggcggtgct cattcgtaat gcgcgtcgtc gatcacgatg
     1981 cggatgaact ctccctcgcg ctcgaatcga tcggagccga agtaccagcc catccgcatc
     2041 cgcgtcggga tggtgtagcc ctggaaagcg gcctccgatt ccatcacgcc gccgaaagtc
     2101 tcgtaccgga agaggcctcc gtccgggttt ccccagcgcg caaccatgac gctctccagc
     2161 cgtccgtcgg atcccagggt gagcgcgagg tccgcgcgct cgccgagcac cgtgaacgcg
     2221 gcgcgcgcgc ggcttgggtc ctgggaggac cacttcacgt cccggccgcg aagcaccgaa
     2281 ggaatccagc acgactcgcc cgccatgcgg ccggcgccgg agcgggcgat gtcatggccg
     2341 gaagccgtca tcaccgggac gagtcccagc agcttccagc gctgctcgcc gattccgggc
     2401 gcgacgcggt cgtatccgga aatggggagc ccgttcatgg acgttcgcgc gcgccacatc
     2461 atctcctggt tccaggtgag cgtttcgtcg gcgccgaagg tccgccacgt cttcagcttg
     2521 atctcgccat gcattcgaag gcgcacggtc cggaccggca tcagtcccga acgatcgagt
     2581 agcagtggca gtcgcggggc gcgtcgctga gattgggaga catcgcccac ttccgcaacg
     2641 tcccctcacg cgccattcca accttctcga ggacgcgcgc ggagcctcga ttgtcggcgt
     2701 cacagaaagc ccacacgcga tggatggggc cctggcccag cgcccattcc acgatggggc
     2761 gcacggcctc gaccatgtat ccctggcccc agtacgggtt gccgaggacg tatcccagct
     2821 cggccctcgg cggctggacc cggatctcga tcatcccgat cagccgatcc gagcccgatg
     2881 gcgtcagggc ccagccgtac cgcccgccct gttccatcgc ttggagcagc cctcgaagga
     2941 actcctcgac cgtatccagc gcccgatggg tcgtgaagcc gagatagcgc gtgacttccg
     3001 gatcggaggc gtactcgaag acgcccggcg cgtcggccat ggtggggcgg cgcaagcgta
     3061 gtcgcgccgt ttcgaacgtc tccggcggat ggcttacggc cgcttgcgcg ctttccacac
     3121 ttcctccgac ggccactgcc gcgcccattc cgccgtgacg aagggatagc gcgtggtcgc
     3181 gccctcgggc gggcgcacct cgacgagatc ctcgacttcg aatccggcgc cgcgcaggat
     3241 gcggaaccat tcgccgtggc tcttgtggaa ttcgacgccc gaatcgccgg gccaggcgat
     3301 ccgccccatt ccgaacgccg ggcgcagcag ccgttcgccg gccgcctgcg tctcgtcgtc
     3361 cggcatgcac agcatcagga gaaacgcatt cgtcaggaag tggagccgcc cgccgggccg
     3421 aagcagccgc gcggcttccg gcacccatcg ttcgggatcg gcccagaggc aggcgccata
     3481 ttcggagatc gcgaagtcga acgaggcgtc gggatagggg accgactccg cgttgccgtg
     3541 gacaagcggg aacgagaggc cgtgctcgcg ctgcaggcgg cgcgcggtcg cgagctgcgc
     3601 ctcggagttg tcgatcccga cgacgcgggc cccccgccgc gcgagccagg acgagacgta
     3661 ggccgtgccg catcccagct cgatcgcgtc gcgctgggag agatcacatg ggagcattcc
     3721 gatctccctc tcggggaggt tccagatgcc ccacgtcggc tcgctcgccg cccagtggcg
     3781 ctcgcccgag gcgacatagt cgctcgcctt ctggtcccag tacgcgcggt tgtggagggc
     3841 gtgctccgga agctcggtca cgcgctcacc gaccgtgtcg gggccttgcg atagagcgtt
     3901 ccgatctggt gttcccgatg cgcggcgaac agggtccgtt ccacggtgcg cgcggaaacg
     3961 ccaagctgtg cggccaggtc ccggatgacc cgaagaaacc ggagccactg atcgaacgtg
     4021 aacccggcgc cccgcgcatt gccctcgacc gcgcccatcg cgtgcaatag ctgccagacc
     4081 cggatgtcga tcacgccgta gcgcttcggc tcgagcatcg tgaggatggc ggaagccgtc
     4141 ggcaccgaga ccccatgcag cgcgagcagc gcatcgaatc gcttccgctc atccacggtg
     4201 gagagcacgg tcgtcgtcgc cttgcgaacg cggtggtggt tgttgccgcg caccaggccg
     4261 atcgagcgcg gcgatttcca gcgacacacc cgctcgaact ctcccttggt gagataccct
     4321 cgcgcgcgcg cggggcgaag ccctgcgatc agatcggccg tgcccggatc ttcctccacg
     4381 atcagccgct cgcgcaggag ccgttccaga gggaggacgg gcgtcctaac ggccacgttc
     4441 cccttccttc cgcggcgcgc gcaccatcgt cgcgagcagg tgctcgagaa gggccgccgg
     4501 gtcggtgcag gcgccggtgt gtacgggcga ggtctggatg accgtgctgc gcggcgccgt
     4561 gagccagcgg aagcgctccc gcttcgacag ggctccgatc ggtcccgcat cgggtccgcc
     4621 cgcgcagatc gcggggaggg tcgctaagtg ggcgcggatc gtggccaggt cggcgccggg
     4681 gtccaatgcg agcaggcggc cctcgtccag ctcgatgcgc gcctccagga aatcgctcgc
     4741 cgggcacgag acgatcgcgc cggcgttgat gaactcctcg cgctccacct tcggtaccac
     4801 gcggacgatg gcgtaatcgt acgggctagg ctcgggcacg aatggcctct tccagaaagg
     4861 cgcgcggcgt ctcgagacgg cgcgccaggt acttcgtgta ggcctcgcga tgctccgagg
     4921 ccgtcgcaaa gggagcgtcg accagccagc cgtccgggat caacgagacg atgcgccgca
     4981 gctcgtcggg agtgatccgc ttctcgagcg ccgcgtccgc ttcctccatc cgggacgctg
     5041 agggcagcag cacgtggtcg cggatcatcg gaaagggatc gcggcttcgc tccagccaat
     5101 gttcccacgt gtggtggaag tagagcgcgg agccgtggtc gatgagccag gtgcgccgat
     5161 gccatacgag caggttcgtg ttgcgcggcg tccggtcgat gttcgtgacg aaggcgtcga
     5221 accagaccac cagcgacgcg aagcccggat cgggccgctc ggccacggga tcgaacatga
     5281 tcgagcccgg caggtagtcg agcgccaggt tcagcccgtc gctcgcccgg atcaggtcct
     5341 ggatctccgg gtcgggctcc gtgcgcgcga ggtcggaatc cagctccacg aagacgatct
     5401 ccggaacggg aagccccgcc gcgcgcgcga tctcgccgca gacgagctcg gcgatcagcg
     5461 ccttggcgcc gtgccccgcg ccacggaact tgagcacgta catgccgtcg tcatcggctt
     5521 cgatgatcgc gggcagcgag cccccctcgc gaagcggcgt gacgtagcgc gtggccgcga
     5581 ccgtgcgcat ctagatgacg cgccgcgtcg cggcggtggc ggggtcgccg gtgttcggcg
     5641 tgcccgtccg ctcgaggaga agcagatgca cctcctcgcg ggccacgggg cggtgctcga
     5701 cgcctcgcgg cacgacgaac agctcgccgg gccccagcgt cacggcgccg tcgcgcagct
     5761 cgagggtgag cgtgccctgg agcacgagga acagatcgtc ggtgtcgtca tgcttgtgcc
     5821 agacgaactc gcccttcacc ttcgccacca tgacgtcgtg gccgttgaac gtggtcacgg
     5881 tccgcggctg ccagtgctcg gcgaacgtgg cgagcttggc cgccaggctc acggctccgc
     5941 tcatcgcccc aggaaccgtt cgatctgccg ccggtggcgc aggatgtgca cgatcgcgtg
     6001 ctcgaagagc tgctcgaagt cgaagatcgg cccccagcgg acggtcatct tcatctcggt
     6061 gcattcctgg tccgtcatgt cccagtgccc ttcgagcgtc tcgaccatgt aggcgagcat
     6121 cttccgcatc tcggccggcg tgtcggcgcg acggaccggc tcgtcccatc ggaccacggg
     6181 ctccttcttc cagtgcgtcc gcatgtagtt cgcgtagccg tacccggccc ggaccacgtg
     6241 tctcatgacg gtctggatcg agcggcagtc ctcgtcctgg gtcacggtgt cgcggacccg
     6301 gtcgaactcc tcgtccgaga tgccctcgag aatgcggatc agctcccccg acgcgcgctc
     6361 gtattcgtcc atcatcgcgc ccacgggtcc cttcctgtag gtcttggcca cggctcctcc
     6421 gcaaggtggt gtgtgggcgg cgattctggc ccaggaaccg gccggaagca aacggctggt
     6481 tccagccacg agcagccctg gcggtgaaga atttcccgcg cgggccgata tcagaagagc
     6541 gtgccgggag ccggggctcc cgtggcgcgc cctcgccgcg aagtcaccca gcatcggagc
     6601 gaaacgtggg aaggaagcac cgctcgttca ggatatcgct gccccccgtc gtgggccggg
     6661 tcgcgaaatc ggccctcaag agcaagctcc tgatggtgct cgtggggctc ttcgtgctgg
     6721 ggttcatcgc gggcaagcag tcggagcccg atcccaccgg cgctccgttc caggagctgt
     6781 tcgagaacct gagcgtcgtc tcctaccgcg actctccctc ggattcggcg tcgcacttca
     6841 tggtcgagct gtcggctcgg gggaaggtct ttcgcgagta cgacgtcgac gagcagcggt
     6901 tcgtggcgcc ggcccacggc cgcgactacg ggcgcatgat cggcgccacg cgctatgagc
     6961 cgctcatcgt gcgcggccac gtggaccgcg gcttctggct ggagctgtcc aacagcaaga
     7021 gccccgagat cctgccggac cagttccagg agatgtacca gaccacgctg gactacgtga
     7081 aaccggtgtc ggtcatcgcg tcgatcgcgg ggacgctctc cggctattcc gtcggatacc
     7141 gcgcgggcac gtggggcacc tcgctcgcca atcccgccgt gcagaagcgg gtgctcgcga
     7201 cctccggcgt cggccggatg atcacgcgcg aggcctggcg gcgcgtgctc ctggagccgg
     7261 tggtgatggg gcacgagagc gatcccgagc ggttcgccgc ggcgcgggag acccagcgga
     7321 tctacacgaa cttcttccgg ctggcgctgg ccgacagcaa cgacttcatc cacaccgagg
     7381 cccggcgcct ggacgccgcg ggctacacgc gcgaggcgat cgcgatgctg gagttcacct
     7441 actcggcgct ccgggcggcc gacgacacgg tgcacctgga gagcgccgat ttccgggccg
     7501 tcgaggaatg ggcgagcctg atcgcgcacc gcggccactg ggctcccacg tcgatccccg
     7561 cttccggcga ggcgcgcgtg cgctatctcg ggacgctggc ctactacggg ctgacgccgg
     7621 tggccgacga cgcgcagcgc gtctgggtgg gcccgcgcgt gctggtgaag gatggcgacg
     7681 aggagggatt cgtcgccgac gacatcccct cgatccgcgc cgcctgcccg ctgacgtggc
     7741 aggactggct gaagcacgac acgaccgggc tcagctcgaa tgagtggacc gcgcagtgga
     7801 tgaaggagtc gaagcagctc gcgcagatgg tgagtttcgg aaagcaggtg gtgcggcaga
     7861 ttcagccggc gaggtagacc cacccccttg acaactgcac aaacgttcac tatcgtcctt
     7921 ttcagccatg aggacgaagc ccaaccccag cggggcccgg ggaaagcggc cccccacgcg
     7981 cacgccctct cccgaggcga tcctcgccta catcgaggag tcgcttcgcg atacgggacg
     8041 cccgccgacg attcgtgaaa tcggagccgc cttcgggatc gcctccacca acggagtccg
     8101 ctaccacctg gacaagttgg agcgggaagg gaggatccgg cgggatcgat tcacttctcg
     8161 aggcatcgaa gtacagttgg ccggcgggcg cggcgcgacg catatggaca ccttgccctc
     8221 catgtcgccc gcgtttcgcc gagccaacgc tagtagcccg gggaccgacg cccgtgcccg
     8281 ggagctcctc gaaatccccc tcctgggcca cgtagccgcc ggtgcgccgc tcctcgccga
     8341 agagcacatc gaggacaccc tgaccctcga cccggccatg gtgcggcccg gcggcaagca
     8401 cttcgccctg cgcgtgcggg gcgacagcat gaagaacgcg gggatcctgg acggcgacgt
     8461 cgtcatcgtc cgccacgaca cccccgtgaa gagcggcgag atcgcggtgg tcctgatcgg
     8521 cgacgaagcc accgtgaagc gcttcttccc gcgacgcgac aaactccttc tgttccccga
     8581 aaacgaagac tacgaaccga tcgaagtgcg tccggccgat cccgacgtcc gggtggcggg
     8641 caaggtggtg ggtgtatttc ggaggctcgg ctgatgctcc atgcgcgatc gtttccatcc
     8701 gagagagacg gctacttcat gatcgagtcc atccacgacc ccatcgaggt catcaccatg
     8761 ttctccggcg gaacgatgaa gccgatccgc ttccgctgga agagcaagac cgtgcgcatc
     8821 gccaaggtga cgggggactg ggtccggcac gagggtgaga acaagatcca ctacttcgcg
     8881 cttctcgcgg acaacgcgga ctactacgag atccgctatg acgcgcgcga gatgacctgg
     8941 cagctcacgc gggtgtggat ggaggggtag ccccgctcca ggtccacgac cgaagcagac
     9001 gcttccgccg gcgctcgggc cagcgccgga agtcctcgag cggcacgaat cccaggacgc
     9061 tgtcgcagtc ctgacgccgg aggaagcgga cccaccccga cagcggctcc cgaacgagaa
     9121 cgacacggtt ggcgaagacc tcctccatcg ccccgatcat ggcgtagagg atggcgccgt
     9181 cggacgccgt gccccagacc aggatcttct cggcagggcc gggagggagg ccgtcgtcgt
     9241 aggcgatcgt cgcgccctcc tcgtagaggt cggcgcgaag ctcatgtcca gggcggcccg
     9301 gcaccggcag gtggaccgac atgtggacct gcgcggaatc cacgctcgga tgcagctccg
     9361 gatggagcgt gcggagacgc tcggccagga gcggtgcgac ccggcgggtc tgctcggtca
     9421 gggtgagcgc gtgcttcaag gagtcgcgct ctcgtcgact tgcgccggcg caagtcgcag
     9481 ctccgagagg ctcctcacga tgcgatcggg gcgggtgccg tttcccggtg ggattcccga
     9541 gccgcgccga tccacccaga tgccgtagat cccgagccgc tggggcgccg cgacgtccca
     9601 ttccaggttg tccccggcca tccaggtgtg gcggggcgag gcctccagct cgtccagcgc
     9661 gatcttgtag acgcgcggat cgggcttgcc gtagcccagc tccccctcga tcaggatcag
     9721 gtcgaacagc gcggccagct ggaaacgcgc gaccttgctg cgctgcgccg ccgcggcgcc
     9781 gttcgtgagg agggccagcc tgcagccctt ctcccgcagc cagcggacgg tctcgatcgc
     9841 atcctccagc ggctccagcc ccatgtgccg ctgcacgcgg tagtgctccg cgatcttctc
     9901 ggcgaggctc tgcgtctcga gtccgatctc cgccagcgaa gcgcgcacca cctcccgacg
     9961 cgcggcgtcc aggtccaggc gcccctcgcg atggcgatcg ggatcggacc agaaccaggc
    10021 gcgcgtgcgc tcgatgacgg cgtggagcgc ggaagggtcg attgcgccca gctcggacgc
    10081 atgagcccgg caggcctcga gccagcagtg ggagacgttc ccgctgtcgt cgaggatcgt
    10141 gtcgtcgaga tcgaggagga tcgcctgggg aaggggttgc gtcacgggcg atgtctcagg
    10201 acttgatccc gcgcacggcc cacgcccgcg cgatcaagtg gatcgatcca tccttctcga
    10261 acggcagccg cgagcggatc cgttcgcgca ggcggccccg gcgcgcctcg tcgagcgtca
    10321 tggcgtacgc cggtgccgga ccctggccgc cgagaaaggg attccagtag tcgtcgaaat
    10381 cgcgaaacac ggtcggcacg tcgatcgtcg tcgtcgccac ggacccaagg cccgcgcccg
    10441 tgaacagcgc ttccagcggc tcgaggcggc agatcgggaa gcgtgggccc tcgtccaggg
    10501 cggcggcatc cggatccagc gccacggcgg cgtcccagaa gtagcgcagc agctccatcc
    10561 gccccgcgta gtcccagacg tacgcggccg cgacgcctcc cgggcacgtc gcgcgcgcca
    10621 tttcggcgac cgcgcgctcc ggctggggca cgaaattcag gacgagcccg gaaacgaccg
    10681 cgtcccggct cgcgtcctcc acgggcagcg cgcgcgcgtc gcccacccgg aacgacacgg
    10741 cctcggcggc gaggccgtcg cggcggacgt gagttcgagc gtactcgatg aacggagccg
    10801 agggatccac gccgaggatg gaggcagggc gcactcggtc gaggatcgtt tgcgccaggg
    10861 cgcccgggcc gcaccccacg tccagccagt gccggccggc cggaaggtcg agccaatcca
    10921 ggaacgaccg cgctgccaga cggctccagc ggccgacgaa cggctcgtag tactttcccg
    10981 ccgcccagac gtcaggactc atggcatcag atccttcatc aaggagagcc cgattccaag
    11041 tcctgcgtgc gacggagagg agtgcggggc ccgcccttga caactgcaca aatgtgcaat
    11101 atcgtaaccc tcgtgatcgg tacccgctcc tcggcccatc cccggtttcg gaccacggag
    11161 tgaccttccg tgcggatcgt ggcgcatgtc gacatggatg cctttttcgc ctcagtcgag
    11221 gagaagctga acccgcggct cgcgggaaag ccgctcgtcg tgggaggctc cagggacagc
    11281 cgcggggtgg tctccgcggc gaactacgcc gcgcgcccgt acgggatcca cgccggcatg
    11341 ccgatcgccg aggcggtgcg cctctgtcct gaagcggcgt tcatcgaagg cgaccccaag
    11401 aaatacgtcc acatctcgct ccagattctg gacacgctca aggacttcac gcccatcgtc
    11461 gagccgttca gcatcgatga ggcgttcctc gacctgaccg cggtggagca gctccgcccg
    11521 cggaaggacg acggcgaccc cgagaccttt ctgaacgcgg cgatcccgct cgcccaggcc
    11581 atccaacgcg ccgtgcaccg gcgcgtgggg ctgacggcct cgatcggaat cgcgcccaac
    11641 aagtacgtgg ccaagatggg gagcggactc cgaaagccga acgggctcac ggttctctcc
    11701 gtacgccgct tccgcgagat cttcgagtcg aaaccggtgt tcgagctgtg ggggatcggc
    11761 gagaagacgg cggcttcgct cgcgacgctc ggcatccgga ccgcgggaca gctcgctcgg
    11821 ttcccacggg agttcctgac ctatcacttc ggactgagcg gcgatcacat gcaggatgcc
    11881 gcgcggggcg acgacgagtc ccaggtggtg ccctacttcg aaggcgtgcc ggtcaagtcg
    11941 atgggtcacg agtacacgct gccggaggac gtgtcggacc gcgagcgcat ccgcgcgcac
    12001 ctccttcgtc tgagcgatca ggtgggccgc cgccttcgcg tcgacggata cctggggcgg
    12061 gtgatcgcgg tgaaattccg aaacgcgcgg ttcaagacca ccatccgcca gcgcgccctg
    12121 gccgaggtga ccgacgacga gaacgtcatc tatggcaccg cgtgcatcct cctggacgac
    12181 aactgggacg gaaagccgct ccgcctggtc ggtgtctcgg tgtcggacct cgtctcggcc
    12241 cagggattcc atcagcacga tctcttctcc accgacgaac gccggcgaag aatgaccgag
    12301 gcggcggact cgctgcggaa ccggttcgga gacgcggtcc tggtgcgggc gggtgcgctg
    12361 gaccagggcg atccgcagcg cctctcgcgc gagggcgcca gccggagcct ggtccgcgtc
    12421 ctcgccgacg agcggcgatg aaggacgagc ccaagatcgt cctcggcacc tcgggctggt
    12481 ccttcaagga ctgggtggga cccttctacc cgccgggaac ggagcgggcg cgcatgctgg
    12541 actactacgt gcgcgagttt ccggccgtcg aggtgaactc cacctactat cggattccgc
    12601 actggcgcac gttcgaggcg atcgcgaaga agacacctcc cgggttcgag atcgtcgtga
    12661 aggcgcatca cgacatgaca cacctccgct cccgggaatc cgacctctat cgggacttcg
    12721 accgctccgt ccggccgctg cgcgaggcgg ggaagctcac ggggatcctc gcgcagtttc
    12781 cctacgggtt ccggcgaacc cgtgagaacg aggagttcct tcacgcgctg cccgacctcc
    12841 tcggccgcga ggtaccgctc ttcgtggagt tccgccaccg atcctgggtg gacgaagccg
    12901 cctttcggga gctccaacag gagggccttg gcttcgtcag cgtggacgag ccggacctgc
    12961 ccggcctggt cccgccgatc gcgcgccgca ccgggccgat cgggtatgtc cgctttcatg
    13021 gcaggaacaa ggagaagtgg tggggggtcg agtcgcgcga ccgcggggcg cccgaccggc
    13081 ccggagcgaa gcgggatgcc gcgaagagcg ccgtgcccga tgcccgggac aaggcccgca
    13141 cacgagtccg cgccggtgcg gacttaggcc cgctcttcag cggcactccc gaccatcccg
    13201 ccgaggcgcc gcccctgccg gcgcctcccg cctcggagcc gcgcacgtcg agcacgcgct
    13261 acgactggtc ctacaccgaa gacgagctgc gcgagtgggt cgacaagatc cgcgagatga
    13321 cgcagcaggc caagaagacg ttcgtcttct tcaacaactg ccacgtgggg caggcggcga
    13381 ccagcgccaa gctgatgcgg aagctgctcg agggggaggg gatgctgtga tccggggcgc
    13441 gcgcggggcg gggacatgac gggcgggggc gtcgcggggg ggagcgatct cgtccacggg
    13501 ccggggctct tcgacgccga gcgcggcgag cgcgggcggc gctccggcgc tcccgagacc
    13561 ctcgacgagt acgaagcgct tctgcacgcg ctcacttcgc gggaccggcg attcgtccac
    13621 ggtccggacg ggctgcgccg ccgtcagaac gagctggtgc acgtggagcg ccttccggcc
    13681 cggcccgccg tgtacggcga cctggcccag cccttgccgc aagcgctcca ggccgtgctg
    13741 cacacggcgg gcatcgagcg cctctacacc caccaggccc gggcggtgga gctcgcgcgc
    13801 tccggggcga acgtcgccgt ggcgacgggc acggcgtcgg ggaagtcgct cgcctatcac
    13861 ctgccgatcc tcgagaggct tctcctcgag ccgggggcga ccgcgctcta cctgtttccg
    13921 accaaggccc tcgcccagga tcagctccgc ggcctcctgc gcttcggcgc ggcgtctccc
    13981 acgctcgggc gctcgctcgc gaccggcacc tacgatggag acacgcccgg cgaagcgcgc
    14041 cgcaagcttc gggagcgcgc caacgcgatc ctcacgaacc ccgacatgct ccaccagggg
    14101 atccttccct accacgcgcg atggagccgg atcttctcca acctgcgcta cgtggtggtg
    14161 gacgagatgc atacctatcg cggcgtgttc ggatcccacg tcgccaacgt gctccggcgg
    14221 ctgaagcggg tggcgcgcca ctacggcgcc gatccccgct ttctcctgag ctccgccacg
    14281 attcgaaacc cgggcgaatt ggcggagtcg ctggtcgggg agccggtcca tgtcgtggac
    14341 gaggacgggg cgccccgggg gcccaagctc ttcgccttct ggaatcccaa gcacgccggt
    14401 cccgaacagg cgggcgcgcg cacatctacg tcgatcgaag cggagcgcat cctggtctcc
    14461 ctcatccggc gccaggtcgc gtcgatcgcg ttcacacggt cgcgcgtggc tgccgagctg
    14521 atctaccgct acgcgcgcga gcagctagag gaacggggcc gccgccgtgc cgataagacc
    14581 gacgggggac cgaactcgct cgccgaccgg ctgagcccct atcgcggcgg ctacctcccc
    14641 gaggagcgcc gcgccatcga gcgccgcctg ttcgacggcg atctcctcgg cgtgatctcg
    14701 acgaacgcgc tcgagctggg gatcgacgtg gggtcgctgg acgcggcggt gctcgtgggt
    14761 ttccccaaca ccatcgcgag cacctggcag caggcgggac gcgcggggcg gtcccaggcg
    14821 ccctcgctcg cggtcgtcgt cgcctacgac gatccgatcg accagtacct gatgcgccac
    14881 cccgaatact tcttcgccca atcgcccgag agcgccgtcg tggatccctc gaatccctac
    14941 gtcctcgcgt cgcatctcgc ctgcgccgcg cacgagctgc cgctctcgcg cgaggacgag
    15001 gcctggttcg gaccgcgcac cgaggcgctc ggccgcctgc tggaagagga aggcaccgtc
    15061 aagtcgctcg acggcctccg ctactggtct tcgtcggagt atccggcggg gcgcgtcaac
    15121 ctccgcacca tctccgacga cacctacacc atcctcgagc ggaagcccgg cgtccccgac
    15181 gcggaggcga gaaccctcgc gacggtggac gcgatcagcg cgccggagct gatctatccc
    15241 gaagcgatct atctgcacga gggagacacc tggttcgtgc gggagctgga cctgcagcag
    15301 aaagtggcct acgtcgagcg gcaggaggtg gactactaca cgcagccggt gctggatacc
    15361 cgtctgcgcc tgaccgagac ccgggagacg aagcgttgcg gcgagtgtga ggtgggcctc
    15421 gggacggcga acgtttcctg ggccaccgtc gccttcaaga agatccgctt tcggagcctg
    15481 gacgcgatcg ggtatcaccc tctcgatctg ccgcgcctga acctggacac ggtggcgctc
    15541 tggatccgcc ccgacgaatc ggcgcgcgcg gcactgcgcg tgaaggggat gaatcccgcc
    15601 gagggaatgt cggggctccg caacctcctc atcacgctgc ttccgctcca cgccatgtgc
    15661 gaccgccccg atctgggtgg cgtgctcgac agcgccaacc tcggcgagca ggcgatcttc
    15721 ctctacgacc gctacccggg cggcctgggt tacgccgagc gcggctacgc catcgtggac
    15781 gacctcctcc gatcgacgct ccgcatgatc gaggactgcc cctgcgaaat cgggtgcccc
    15841 tcgtgcgtcg gcctgcctgt cctccgcccg gcgcagcaac aggactacga cctgcacggc
    15901 ggctggccca tcccgaacaa ggccgccacg gagacgctgc tccggcgcct cctcggcgcc
    15961 tgacgcgcgc cgtccccggc tccggagata cgcgagatgc tctccaagac gctccgcgat
    16021 cggatcgggg gcctcctccg gcagggggcg ctggacgtac cggccccgct ctcgaacggc
    16081 ggcatgaacg gccatgcggt gggcgcttcc gacaaggccg gcgccgcgcc ggagcgtttc
    16141 gtccccgaac cgcgccgcca tttcgtcgcg accggcatcg aggagatcct tcccggcggc
    16201 gtgatcgata cggcgcaggg gaccgttttc gcgcacgagc ggctctacac cgatttgggg
    16261 gagcgtcccg ttccgctcct gcgccggctc gccgagctct gcgcctatcc ggggtgcgcg
    16321 ccggagccga gcagagcggg cccgcactgg gtggagatcc ccggcgtcga ctgcccctat
    16381 cccgcgctcc cggaacgcgc gcgcgcccgc cggcggcggc cccgcgatct ggcggaagcg
    16441 accggacttc acgaccctcc cgagcaggga ctgtttcgcc gattcggata ccggcgggtg
    16501 ctctatctgg acatcgaaac gtgcggcctc tcggcggcgc cggtgttcct ggtcgggctc
    16561 tgcatggtgg gggagcggaa tctcatcctt cgccagctct tcgcgcgcga ctacgccgag
    16621 gagcgggcgc tgctcgtgga gctcaagcgc ctggccggcg agcacgactt tctcgtgacc
    16681 ttcaacggca aggcgttcga cgtgcccttc cttcgcgatc gcgccatcca tcaccggctg
    16741 gagcccccga tcacgatgcc ccatctggac ctgctctgga tggcgcgccg gcgctggaag
    16801 gatctcctcc ccgactgcaa gctgaagacg atcgaatggc gcgtgctgcg gcgtaggcgc
    16861 gcgggagacg tgggcggatc ggagatcccc gggctctatc acgattacgt gaagcggggc
    16921 gaggcgcacc gcctgcttcc ggtgttccat cacaaccttc tcgacgtggt cgcgatggtg
    16981 gagctgacgc cggtgatctt cgatccggac gtggggacct actagagagg gggcggggcg
    17041 cctatgcgga gtttcgcgcg ctccaccgct cgcgcgtcag ctcatagcgc agcgagccgg
    17101 gaccggccgg ggcctcgtcg gtccgaacga agccgctctt ctcgagcacc cgctgcgacg
    17161 ggcgaagctc cggcagcgtc tcggcgacca cgcggcggac gcgcgggtcg ccgaaggccc
    17221 acgcgagcgt ggcgcccacc gcctccgtgg ccagtcctct tccgtagtac tcctcgacga
    17281 tggagtagcc gatctcgatc gtgccttcgg cgtcgggcgg tcccttgaag ccgccccctc
    17341 cgatcaggag cggccgccgc tccggcgggg ccacctgggc gcgcaccagc caggaccacc
    17401 cgaggaaccc gaccgcctcc gggtgctcct cgagctgacg gcaaagaaaa tcgcgccctt
    17461 cctgatcgtt ctcccctggg ggccaggcct cgtggatctc ggcacccagc agacgggcca
    17521 gctcgccgcg gtcggtcgcc tccgcacggg cgatggccgg ggtggaggcg atcaggagga
    17581 ggcgctcggt ttgaagttgg atcggctcgg tcacaaccgt atcctttcat gc
//
LOCUS       DASSLZ010000151         4553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_685582, whole genome shotgun sequence.
ACCESSION   DASSLZ010000151 DASSLZ010000000
VERSION     DASSLZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685582"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VFM00_05865"
     CDS             complement(<1..760)
                     /locus_tag="VFM00_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497806.1"
                     /translation="MIPKRGTALSYEEAERLLGGDAAGLPPIVVAAGPDEYLRERLVD
                     AFRAGGEREGSDFCRLEGDDLEPDVLAEALASISLFSSSRRIWIREAAKLDKACEESL
                     LAWARGSAEGARILVTTARETPELRTLQALAASGVSVACALRPGEAARWVARMIEDAK
                     LAVPPGAAEALAGAARDLLEARQEVEKLRVHAEPSGKVPPRALGALRGARAGGSLDRW
                     ADAVLARDAAAARREAAALDAEGVGGGNALWAVAE"
     gene            complement(760..1278)
                     /locus_tag="VFM00_05870"
     CDS             complement(760..1278)
                     /locus_tag="VFM00_05870"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HET7497807.1"
                     /translation="MKCCRLLLQLGLVALAPWMMSGCGYTLSSVVPGYIKTIAIPVFQ
                     NSTVEFGIAEDVTSAIQTAFQGNGRLTAAGEKQADSVLRGTVLAYRNQVFGYTQQERA
                     TSYEVVLTVRVLYRDLVKSRDIWKEDALTVRTTYNVVAQGGVPARTETDARAEVVRKL
                     ADQIVSRTVQGW"
     gene            complement(1265..2698)
                     /locus_tag="VFM00_05875"
     CDS             complement(1265..2698)
                     /locus_tag="VFM00_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7497808.1"
                     /translation="MAKASILIIDDDETIRYFLPRDLEAEGYQVTTAESGNLGLRALE
                     RDTVDLVLLDIRLPDRSGIDVLQRIRTQWPDQAVVMLTGEPDHETAVQAMRLGARDYL
                     TKGKPIREELLAVLERELATQQLGREVQHHRQQKSQHFSREFVRGQSASMARVYAIVE
                     QVAASDSTSVLIEGESGSGKELIAHLIHELSGRREKPMLEVNCAAIPRELLESELFGH
                     EKGAFTDARQQKQGLLELANGGTLFLDEVGEMSLQIQVKLLRVLERMTFKRVGGTKDI
                     TVSVRVISATNQNLETMVREQRFREDLYYRLKVVPIRVPPLRERREDILPLANHFLAQ
                     FNRAFNKGFERIEPEADRVLLSYPWPGNIRELRNLFERIVLLNTGDRIQAQHLAAAIA
                     PPAARTGSSLDALRAVIESGQFAEEGIDLETALGEVEREVIQRAFDWTRGNQSRAAQL
                     LQMKRDKLRYRMKLYGFHDATAPGEVL"
     gene            complement(2712..>4553)
                     /locus_tag="VFM00_05880"
     CDS             complement(2712..>4553)
                     /locus_tag="VFM00_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7497809.1"
                     /translation="PRPTQRAAVERLWRRAEEIRSLCGLPPLTGQEQGAPESGDAVAL
                     EPLVDILLRDLETNFRRELATRVQLEGLGELVAFAARDAEPGRPYQILVNYLARALDE
                     PRLWLGTVDGSPASFTVYRAGDPAVMDVSPDRVQVHWLQPDWLHWIALGDGNRQLWIG
                     PSGSRKGGPWHPIPVRGEIAVDRLTGDVPICPGAVGGGKSCGLSRLPLEEMAGGHRAC
                     GRCQFRHVVALIGIEGADARQRLSQVEAIAPSLGPILVNLGLKEVLDLEARFRDEVIE
                     NLPLGVVAIDTRGRVLAWNRSAETIVGLDREQVRSSPLPRLLAERSWHEVLVRSLEMG
                     ETTVGAEHDVVRADGSSVPVEVSTAPLRDAEGRIRGAVATLLDVSSLREMEDRIRQLD
                     RLAALGRFASSVAHELRNPLTGIATGVQYLSRGFPEGDERHESVAFILREVVRLNTII
                     QDLFTATKPRAILPRPTRVADVVEWAVRGLSPAIEASGVTIEREGADTWPTVTADADQ
                     LQQVLLNLIQNAVQASPKEGTVTIRAYRRPGERPSVAIQVEDRGHGIPADLLPRIFEP
                     FFTTRPKGTGLGLFVAHGIVQRHGGTLEVESDAAGTRFRVILPEAPE"
BASE COUNT          657 a         1620 c         1580 g          696 t
ORIGIN      
        1 gttcggccac ggcccagagc gcgttcccgc cgcccacgcc ctcggcatcg agagcggccg
       61 cttcgcgccg cgccgccgcg gcgtcccgcg cgagcacggc gtccgcccag cgatccagcg
      121 acccgcccgc gcgcgctccg cgcagcgccc ccagggcgcg aggcggcacc ttgcccgagg
      181 gctcggcgtg cacgcgaagc ttctccactt cctggcgggc ttccagcagg tcgcgcgcgg
      241 ctccggccag cgcctcggcg gcgccgggcg gaacggcgag cttggcgtcc tcgatcatcc
      301 gggcgaccca gcgcgccgcc tcccccggcc ggagcgcgca ggctacggag acgcccgacg
      361 ccgccagggc ctggagcgtc cggagctccg gggtctcgcg cgcggtcgtg acgagaatgc
      421 gcgcgccctc ggcggagccg cgcgcccatg cgagcagcga ctcctcgcac gctttgtcca
      481 gcttcgcggc ctcgcggatc cagatccggc gcgaggatga gaagagcgag atggacgcga
      541 gcgcctcggc gagaacgtcg ggttcgagat catcgccctc gagacggcag aagtcggatc
      601 cttcccgctc ccctcccgcg cggaaggcgt ccacgaggcg ctcgcgcagg tattcgtcgg
      661 gacccgccgc caccacgatg ggcggcagtc cggcggcgtc cccgcccagc aggcgctccg
      721 cttcctcgta ggatagcgcg gttccccgct tcggtatcat caccacccct ggacggtgcg
      781 gctcacgatc tgatcggcca gcttgcgcac gacctcggct ctcgcgtcgg tttcggtccg
      841 cgcgggaacg cccccctggg cgacgacatt gtacgtggtc cggacggtga gcgcgtcttc
      901 cttccagatg tcccggctct tcacgagatc gcggtaaagc acgcgcacgg tgaggaccac
      961 ctcgtacgac gtcgcccgct cctgctgggt atagccgaag acctggttcc gataggcgag
     1021 cacggtgccg cggagcacgg aatcggcctg cttctccccc gccgccgtga ggcgtccgtt
     1081 tccctggaag gcggtctgga tcgcgctggt cacgtcctcg gcgatcccga actcgaccgt
     1141 gctgttctgg aagaccggga tcgcgatggt cttgatgtag ccggggacca cactcgagag
     1201 cgtgtagccg catccggaca tcatccaggg ggcgagcgcg acgaggccga gctggaggag
     1261 gagcctacag cacttcaccc ggggccgtcg cgtcgtggaa tccatagagc ttcatccgat
     1321 agcggagctt gtcgcgtttc atctgcagca gctgggcggc acggctctgg ttgccgcggg
     1381 tccagtcgaa cgcgcgctgg atcacctcgc gctccacctc gccgagcgcc gtctcgagat
     1441 cgatcccctc ttcggcgaac tgcccgctct cgatgaccgc gcgcagcgcg tccagggagg
     1501 agccggtgcg ggccgccggc ggcgcgatgg ccgccgccag gtgctgcgcc tggatccgat
     1561 ctcccgtatt gagaagcacg atccgctcga aaaggttccg gagctcgcgg atgttcccgg
     1621 gccagggata ggagagaagc acacggtccg cctccggctc gatccgttca aatcccttgt
     1681 tgaacgcccg gttgaactgc gcgaggaagt ggttcgccag cgggagaatg tcctcgcggc
     1741 gctcgcgcag gggcggcacg cggatcggca ccaccttgag ccggtagtag agatcctccc
     1801 ggaagcgctg ctcgcggacc atcgtctcga ggttctggtt cgtcgccgaa atgacgcgca
     1861 cgctgaccgt gatgtccttg gtgccgccga cccgcttgaa cgtcatccgc tcgagcacgc
     1921 ggaggagctt cacctggatc tggaggctca tctctccgac ctcgtccagg aagagcgtgc
     1981 cgccgttcgc gagctcgaga agaccctgct tctgctggcg ggcgtcggtg aaggcgccct
     2041 tctcgtggcc gaacagctcc gattccagga gctcgcgcgg gatcgccgcg cagttcacct
     2101 cgagcatcgg cttctcgcgc cggccggaga gctcgtggat gagatgcgcg atcagctcct
     2161 tccccgaccc gctctccccc tcgatcagga cgctggtgga gtcggaggcg gccacctgct
     2221 ccacgatggc gtagacgcgc gccatcgacg cgctctggcc gcgcacgaac tcgcgggaga
     2281 agtgctggct cttctgctgc cggtggtgct gcacctcgcg gccgagctgc tgcgtggcca
     2341 gctcccgctc cagcacggcc aggagctcct cgcggatcgg ctttcccttg gtcaggtagt
     2401 cgcgggcgcc gagccgcatg gcctgcaccg cggtttcgtg gtcaggctcg ccggtcagca
     2461 tgacgacggc ctggtcgggc cactgcgtcc ggatccgctg gagcacgtcg attccgctcc
     2521 ggtcgggaag gcggatgtcg aggagcacga ggtccaccgt gtcgcgctcg agcgcgcgga
     2581 gtcccaggtt gccgctctcc gccgtggtga cctggtaccc ctccgcctcg agatcgcgcg
     2641 ggaggaagta gcggatcgtc tcgtcgtcgt cgatgatcag gatcgacgct ttggccatgc
     2701 ccgttgggtc ctcattccgg tgcttccggc agaatgacgc ggaagcgggt tcccgcggcg
     2761 tcgctctcca cctcgagcgt tccgccgtgg cgctgcacga taccatgcgc gacgaagagc
     2821 ccgagtccgg tccccttggg ccgggtggtg aagaagggct cgaagatgcg cggcaggagg
     2881 tcggccggga tgccgtgccc tcggtcctcg acctgaatgg cgacggaggg ccgttccccc
     2941 ggacgccggt aggcgcggat cgtgaccgtg ccctccttcg gcgaggcctg gaccgcgttc
     3001 tggatcaggt tcaggagcac ctgctggagc tggtccgcgt cggccgtgac cgtgggccat
     3061 gtatcggcac cctcccgctc gatcgtgacc cccgaagcct cgatggccgg cgagagcccc
     3121 cggaccgccc actccacgac gtcggcgacg cgggtggggc gcggcaggat ggcgcgcggc
     3181 ttggtggcgg tgaagaggtc ctggataatc gtgttcaggc gcaccacctc gcggaggatg
     3241 aacgccacgc tctcgtggcg ctcgtccccc tcggggaaac cgcggctcaa gtactggacg
     3301 ccggtcgcga tgccggtaag ggggtttcga agctcgtgcg ccacgctcga ggcaaaccgc
     3361 cccagcgccg cgagccggtc gagctgacgg atccggtcct ccatctcgcg caagctggag
     3421 acgtcgagca gcgtggccac cgcaccccgg atccgcccct cggcgtcgcg cagcggcgcg
     3481 gtgctcacct ccacgggaac gctcgagcca tccgcccgga cgacgtcgtg ctccgcaccg
     3541 accgtcgtct cccccatttc gagcgaccgc accagcacct cgtgccagga gcgctccgcg
     3601 agcagccgcg gcagcgggct cgagcggacc tgctcgcgat ccagcccgac gatcgtctcg
     3661 gccgagcggt tccaggcgag cacgcggccc cgcgtgtcga tggcgaccac gccgagcggg
     3721 aggttctcga tcacctcgtc gcggaagcgc gcctcgaggt cgagcacctc cttcaagccg
     3781 aggttgacga ggatcggtcc gagcgaaggg gcgatcgcct ccacctgcga gagccgctgg
     3841 cgggcgtccg ccccttcgat cccgatcagc gccacgacgt ggcggaactg gcagcggccg
     3901 cacgcgcgat gccccccggc catctcctcc agtggaaggc gcgagaggcc gcaggacttt
     3961 ccgcctccga ccgcgcccgg gcagatcggc acgtcgcccg tgaggcgatc caccgcgatc
     4021 tcgccgcgaa ccggaatcgg atgccacggt ccgcctttcc gcgagcccga gggaccgatc
     4081 cagagctgcc ggtttccgtc cccgagcgcg atccagtgaa gccagtcggg ctggagccag
     4141 tggacctgca cgcggtcggg gctcacgtcc atcacggcgg gatcgccggc gcgatagacg
     4201 gtgaacgagg cgggactccc gtcgacggtg cccagccaga ggcgcggctc gtcgagggcg
     4261 cgcgcgaggt agttcacgag aatctgatag gggcggccgg gctccgcgtc gcgggcggcg
     4321 aaggcgacga gctcaccgag accttcgagc tgaacacgcg tagccagctc gcgccggaag
     4381 ttcgtctcga ggtcgcgaag gaggatgtcg accagcggct cgagcgcgac ggcatcgccg
     4441 ctctccggcg cgccctgctc ctgtccggtg agcggcggca gaccgcagag cgaacgaatc
     4501 tcttccgcgc ggcgccaaag gcgctcgacg gcggcgcgct gtgtgggtct cgg
//
LOCUS       DASSLZ010000152         8899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_433517, whole genome shotgun sequence.
ACCESSION   DASSLZ010000152 DASSLZ010000000
VERSION     DASSLZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433517"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="VFM00_05885"
     CDS             complement(<1..1407)
                     /locus_tag="VFM00_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HET7497810.1"
                     /translation="MRPLLVAKTWFQAAILVFLFGFFVLGFLAMRTYQAEPPIPKQVQ
                     DESGRVLFTGADVTAGQQVFLKNGLMEYGSIFGHGAYLGPDYTADYLRRSATMVRDAY
                     GGATSDRAHDQTVAAFRTNRYDPAAGVLRFDAARSAAHDSLVAHYAAFFSEPSTRFGL
                     RPRAITDPRELKQLTAYFAWTAWAASTRRPGKEYTYTNNWPPEPLVENRPTADVLVWS
                     VLSLITLLGGTGVMLAVFGRWRFLGWHGRDQERIGFRAPGEVALTPAQRATAWYFLVM
                     GLLFLGQALLGGSVQHYRAEIAGFFGIDLARILPFNLARTWHLQLAIFWVAASYLAAG
                     IFIAPMIARTEPKGQHYLAYGLLGAVAVVVFGSLAGEYAGIFGYAGRLWEWFGNQGFE
                     YIDLGRFWQALLSVGLVLWVVILYRVLRTRLHQEHKGNMPWMFFYAALAIPAFYAVGL
                     LAQTASHVTSAEYWRWWVV"
     gene            1504..2079
                     /locus_tag="VFM00_05890"
     CDS             1504..2079
                     /locus_tag="VFM00_05890"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HET7497811.1"
                     /translation="MNPETATLQTEIDRLERELAEGRERLAALKRKAPRHAVKDYTLA
                     GWNGPVQLSELFAGKPDMIVVHNMGRGCRYCTLWADGFNGVYPHLADRAAFVVCSPDP
                     VEVQKEFASGRGWRFPMVSGKESTFIQDMGFRSEKGWMPGVSTFHLEPTGGVVRVAQA
                     TFGPGDPFCAVWHLFDLLAEGVNEWEPQYRY"
     gene            complement(2076..3212)
                     /locus_tag="VFM00_05895"
     CDS             complement(2076..3212)
                     /locus_tag="VFM00_05895"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7497812.1"
                     /translation="MSDHFAYPLALMVNPPAPRARSRSLARKFKPLSEFIRARIADGL
                     IPGAVVAAGRRGRVVYHAAHGHRALRPRRERMTVTTIFDLASLTKVMATAPVLVEHAA
                     RGAFSLLDPLERHLPETAGTEVGRIPLHRLLTHTAGFVPDNPLPDYGGSKRALFAAIA
                     REPLEAPPGARFTYSDVGYVLAQGVAERRLRKRLDALAEELLFVPLRFRDTRFGTRRK
                     DRGRVAPTERVGASWLRGMVHDPRARTRALAGVGGHAGMFGTAREVARFCEMILRRGL
                     MGRRRILAEETVRAMTTDQCEGNLGVRRGFGFDIESPYSAPRGATFSRASFGHSGWTG
                     VSLWIDPEMDAYLVLLTNAIHPDGHKDLKAFRYEAATLAAKAMR"
     gene            3263..3943
                     /locus_tag="VFM00_05900"
     CDS             3263..3943
                     /locus_tag="VFM00_05900"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7497813.1"
                     /translation="MIPLRDENRSGSFPIVNVLLIAACTVVFLYEISLPTGGLKAFFD
                     QFALTPGRVSYQLGSGEGSWTEVIAPFFTSMFLHGGWLHLIGNMWFLWIFGDNVEDSM
                     GSVRYLLFYILCGLGAGTAHYMLGPGSRLPTVGASGAIAGVLAGYVVMFPGARVLTLV
                     PLGFFIRVMELPAIAVIGIWFLIQIVSSALSFGVADKGGVAFSAHVGGFVAGLLLVWL
                     FRRRRVVV"
     gene            4081..4491
                     /locus_tag="VFM00_05905"
     CDS             4081..4491
                     /locus_tag="VFM00_05905"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET7497814.1"
                     /translation="MSSRLRIIVVSLVIIGAISYLVLTGVKQTGLQYMTVAELAQLQK
                     PPSQGGFRLDGKVAAGTIDYNQKIPVLNFQMTDGKEAIGVVYDGLMPDAFGEGREVVV
                     EGTYDQSARTLHASKLVTKCPSKYEAEGLGKDKT"
     gene            4488..6449
                     /locus_tag="VFM00_05910"
     CDS             4488..6449
                     /locus_tag="VFM00_05910"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HET7497815.1"
                     /translation="MSEIGKLSVILAFLASCYAVVSIAYGLRTSMSGPLRSGRRAVWT
                     VCGLTLLSVVLLVYKLVNLDFSWRYVAEHTSRDLPMMYRVSSLWAGQEGSLLLWLLIL
                     SAYACAFLWAYRKRLDPFYDAVAMVVACVMVFFTSLLTFVSSPFRVLATPPPDGNGLN
                     PLLQDPGMMIHPPILYTGYVGFIIPFAFGIAVLLMNRTGTRWIEEVRRWTLFNWMFLG
                     TGILMGARWAYIELGWGGYWGWDPVENASLMPWLVGTAFLHSVMIEQRRGMLKTWNVA
                     LIVLTFELSIFGTFLTRSGVLTSVHSFTESNIGPWFMGFILISSTIAAALILYRKALL
                     ESENRLDAVVSREGSFLFNNVLFVALCFATFLGTIFPVVSEAVTGSKISVSAPFFNRV
                     NVPIALALLLLTGAGPVLSWKRATASVLKRNLVIPTFVGVLAAVIGIPLGLHGLYPII
                     CVFGVAFVVSTIVMEFARGLQARKDVAEAKGAMAVVSLVQKNKRRYGGYIVHAGMVLV
                     FVGVLGSTVFQKEAHGPLREGDKLSVGPYALTLRGVEEKQVANAQQTRAVLDVNRNGK
                     SLGLAHPAKSMYFKSQQPMTEIALHQTPSEDLYMILGGVNDDGSVSIQAYINPLVSFI
                     WLGGVIMVFGTIIALTDKMRLRREEKLGG"
     gene            6446..6958
                     /locus_tag="VFM00_05915"
     CDS             6446..6958
                     /locus_tag="VFM00_05915"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HET7497816.1"
                     /translation="MTRALRLLLGVLVGSVLLSGALSAPAYAKKDPAKTITDGIICPC
                     SCGEILTGCTCETGKAMRAYVDDEVKGGKTKDQIEAALVNQYGEVILGAPKAKGFNLL
                     VWAAPVVATVMGLLIASFVLVRWRRRNQAAAPAAPPADGPDAPFRTDPDARDAALRAR
                     AEEEYRRLQE"
     gene            6975..7364
                     /locus_tag="VFM00_05920"
     CDS             6975..7364
                     /locus_tag="VFM00_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497817.1"
                     /translation="MIALAQVCLALAVALFVLSPLWFYRGQRAHIEAADASVRRSIAE
                     KKGRLYSQLLDLDFDKDSGKISPEDHARMREETMSEVLAVLAEEDRLGGPRRGLKPTV
                     IEGGDRVERMIEEYKRARPAAIEVNRS"
     gene            7361..8680
                     /locus_tag="VFM00_05925"
     CDS             7361..8680
                     /locus_tag="VFM00_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497818.1"
                     /translation="MTKQTLRILALPAAVLLLALVLALLAIPAWAVTITGHVLKGAER
                     APVKDAPVSIHVVRGNEELPGGTVPSDPEGGFRFTGITEGAGLEYYLSTEYEGAFYTE
                     GPLAIQNGAASQDMQVYEVGKNFAAVKVQDHHVIVERKEDGLHVTEILIFQNDANTAY
                     LGVGANHAMPSGMTVGLPASIKDFKPGLGLDEPSVHVQGREMMSMRPIPPGQRPLSFT
                     YTIPLSGRMDLSHRLYFPTRQMTVLLDDPKLHVESTQLTANGTRQQGGKTYAMYIGSE
                     LPVGAEVSMRVGGAGFFSNPKVYPFMAAPFLIAAALWFASRRGSRHMKEKHMAAQAAG
                     HPAGAPSGPASRAPVASTGGTGIGPSAGEIGTAASTMSTAGKPRHSPSANGGHNEMAD
                     TYLYLIAALDQGVERGDVSPESHALIRGNLKRRLETILADEPHAKAR"
     gene            8689..>8899
                     /locus_tag="VFM00_05930"
     CDS             8689..>8899
                     /locus_tag="VFM00_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7497819.1"
                     /translation="MIAGAPPISGAVLRALGIRKRFESRWILRDASIALAAGDVVLLA
                     GRNGSGKTTLARILATLLEPDQGEVL"
BASE COUNT         1404 a         2975 c         3064 g         1456 t
ORIGIN      
        1 caccacccac cagcgccagt actcggcgct cgtcacgtgc gaggcggtct gcgcgaggag
       61 tcccaccgcg tagaacgcgg ggatggcgag ggccgcgtag aagaacatcc agggcatgtt
      121 gcctttgtgt tcttgatgta accgcgtgcg aagcacgcgg tagagaatca ccacccagag
      181 caccagaccc accgagagca gtgcctgcca gaagcggcca aggtcgatgt actcgaatcc
      241 ttgattgcca aaccactccc agaggcgccc ggcgtagccg aagatgcccg cgtattcgcc
      301 cgccaggctg ccgaacacca cgacagccac ggctccgagg agcccgtacg ccaggtagtg
      361 ttgccccttc ggttcggtgc gcgcgatcat cggggcgatg aagatccccg ccgccagata
      421 ggaggccgcc acccagaaga tcgcgagctg gaggtgccag gtgcgcgcga ggttgaaggg
      481 aaggatgcgc gccaggtcga tgccgaagaa ccccgcgatc tcggcgcggt agtgctgcac
      541 ggatcctccg agcagcgcct ggccgaggaa gaggagtccc atcaccagga agtaccaggc
      601 ggtcgcccgc tgggccggcg tgagcgcgac ctcgcccggg gcgcggaagc cgatccgctc
      661 ctggtcgcgg ccatgccacc ccaggaagcg ccatcgtccg aacacggcga gcatgacgcc
      721 cgtgccgccg aggagcgtga tcagggagag gacgctccag acgagcacgt cggcggtcgg
      781 gcggttctcg acgagcggct cggggggcca gttgttcgtg tacgtgtact ccttcccggg
      841 ccgccgcgtg gacgcggccc aggcggtcca ggcgaagtag gccgtgagct gcttgagctc
      901 gcgcggatcg gtgatggcgc gggggcgcag gccgaagcgg gtgctgggct cggagaagaa
      961 ggcggcgtag tgcgcgacca gcgagtcgtg cgcggcggac cgcgccgcgt cgaagcgcag
     1021 gacgccggcg gccgggtcgt agcggttggt ccggaacgcc gccacggtct ggtcgtgcgc
     1081 gcggtcggag gtcgctccgc cgtaggcgtc gcgaaccatc gtggccgatc ggcggagata
     1141 gtccgccgtg taatcggggc cgaggtaggc tccgtgcccg aagatcgagc cgtattccat
     1201 gagaccgttc ttcaagaaga cctgctggcc cgcggtgacg tcggcgccgg tgaagagcac
     1261 gcggccgctc tcgtcctgga cctgtttcgg gatcggcggc tcggcctggt aggtgcgcat
     1321 cgcgaggaag ccgaggacga agaagccgaa cagaaagacg aggatggcgg cctggaacca
     1381 ggtcttcgct acgaggaggg gtcgcatggg ggcctccgtg atagactcgc gcgcgctacg
     1441 ccgaaccggc ccgttcgcgc cgaaccgggg ccctcctggc cccgagagga gggaatccta
     1501 ccgatgaatc ccgagaccgc aacccttcaa accgagatcg accgcctgga gcgggagctg
     1561 gccgaggggc gggagcgcct cgccgcgctg aagcgcaagg cccctcgcca tgccgtgaag
     1621 gactacaccc tcgcggggtg gaacggcccc gtgcagctga gcgagctgtt cgcggggaag
     1681 cccgacatga tcgtggtgca caacatggga cgcggctgcc gctactgcac cctgtgggcc
     1741 gacggattca acggggtcta tccgcacctg gctgaccgcg ccgcgttcgt cgtctgctcg
     1801 cccgacccgg tggaggtgca gaaggaattc gcgtcggggc gcggctggcg cttcccgatg
     1861 gtctccggca aggaatccac gttcatccag gacatgggat tccggagcga gaagggatgg
     1921 atgcccggcg tctcgacgtt ccacctcgag ccgacgggcg gtgtggttcg ggtggcccag
     1981 gcgacgttcg ggccgggcga ccccttctgc gcggtgtggc acctgttcga cctgctggcc
     2041 gaaggagtca acgaatggga gccgcagtac cgctactagc gcatcgcctt cgcggcgagc
     2101 gtcgccgcct cgtagcgaaa cgccttcagg tccttgtgcc cgtcggggtg gatcgcgttc
     2161 gtgagcagga cgagataggc gtccatctcc gggtcgatcc agagggagac gccggtccag
     2221 ccgctgtggc cgaacgaggc gcgtgagaag gtagcgccgc gcggggcgga ataggggctc
     2281 tcgatgtcga atccgaagcc tcgccgcacg ccgaggttgc cctcgcactg atcggtggtc
     2341 atcgcgcgca ccgtctcctc ggccaggatc cgccgccggc ccatcaggcc gcggcggagg
     2401 atcatctcgc agaagcgcgc cacctcgcgc gcggtgccga acatccccgc gtgcccgccc
     2461 acccctgcga gcgccctcgt ccgggcgcgg ggatcgtgaa ccatgccgcg gagccaggag
     2521 gcgcccaccc gctccgtcgg cgccacgcgg ccgcgatcct tcctccgggt cccgaagcgc
     2581 gtgtcccgga agcgcagggg gacgaacagg agctcctccg ccagcgcgtc caggcgcttc
     2641 cgcagccgcc gctccgccac gccctgggcc agcacgtagc cgacgtcgct gtaggtgaag
     2701 cgcgcccccg gcggcgcctc cagcggttcg cgcgcgatcg cggcgaagag cgcgcgcttg
     2761 cttccgccgt agtcgggaag ggggttgtcg ggcacgaagc cggccgtatg cgtgagcagg
     2821 cggtggagcg gaatccggcc gacctcggtg ccggccgtct cgggcaggtg gcgctccagg
     2881 ggatcgagga gcgagaaggc gccgcgcgcg gcgtgctcca ccagcaccgg cgcggtcgcc
     2941 atcaccttcg tcaaggaggc caggtcgaag atggtggtca ccgtcatccg ctcgcgccgg
     3001 gggcgcaggg cgcggtggcc gtgggccgcg tgatagacta cgcgcccgcg gcggccggcg
     3061 gccacgacgg cgccgggaat cagcccgtcg gcgatgcggg cgcggatgaa ctcggagagc
     3121 ggcttgaatt tgcgcgcgag ggaacgagag cgcgcccgag gcgctggagg gttcaccatg
     3181 agcgccagag gatacgcgaa atgatcggac attccagcgc gctccgccgg gacgtttcgc
     3241 ggcggcgcag ggggagctcc gaatgatccc gcttcgcgac gagaaccggt cggggagttt
     3301 cccgatcgtc aacgtgctgc tgatcgcggc ctgcactgtt gtgtttctct acgaaatctc
     3361 cctccccacc ggcggactga aggccttctt cgatcagttc gccctcacgc cggggcgcgt
     3421 cagctaccag ctcggttccg gcgagggctc ctggaccgag gtgatcgccc ccttcttcac
     3481 ctccatgttc ctccacggcg gctggctcca cctgatcgga aacatgtggt tcctctggat
     3541 cttcggcgac aacgtcgagg attccatggg gtcggtgcgc tatctcctct tctacatcct
     3601 ctgcggcctg ggggcgggca ccgcccatta catgctgggc cccgggtccc gcctgccgac
     3661 cgtcggcgcc tcgggcgcca tcgccggggt tctggccggg tatgtcgtga tgtttccggg
     3721 cgcccgggtc ctgacgctgg tgccgctggg gttcttcatc cgggtcatgg agttgcccgc
     3781 catcgccgtg atcggcatct ggttcctcat ccagatcgtg agcagcgccc tgagcttcgg
     3841 ggtggccgac aaggggggcg tggccttttc cgcccatgtg ggcgggttcg tggcggggct
     3901 cctcctggtc tggctcttcc ggcgccgccg ggtggtggtg tagttcggcc ccacggtggg
     3961 tactaccgag gtagtggtac ccggggtata ccaccgtcgc aacttgactc aagcccgcca
     4021 tctgccacaa tcggtaggcg caaagggaac caccctctcc tctaaatcag ggaacgacaa
     4081 atgagtagcc gactccgcat catcgtggtc tcgctcgtga tcatcggggc gatcagttac
     4141 ctggtcctga cgggagtgaa gcagaccggg ctccagtaca tgaccgtggc cgagctggcg
     4201 cagctccaga agccgccctc gcagggaggc ttccggctgg acgggaaggt ggcggcgggg
     4261 accatcgact acaaccagaa gatacccgtg cttaactttc agatgaccga cggcaaagaa
     4321 gcgatcggcg tggtctacga cggcctcatg ccggacgcct ttggcgaggg ccgggaagtg
     4381 gtggtcgaag ggacctacga tcagtcggct cgcacgctcc acgcctccaa gttggtcacg
     4441 aagtgcccct ccaagtacga ggccgaaggt ctcggaaagg acaaaacgtg agcgaaatcg
     4501 gcaagctttc ggtcatcctg gcgttcctcg cttcctgcta cgcggtcgtg tcgatcgcct
     4561 acgggctccg cacctccatg tccggcccgc tgcgcagcgg ccggcgcgcc gtctggacgg
     4621 tctgcggcct gacgctcctc tcggtggttc tcctggtgta caagctggtg aacctggact
     4681 tcagctggcg ctacgtggcc gagcacacca gccgcgacct gccgatgatg taccgggtct
     4741 cctcgctctg ggcggggcag gagggctcgc tcctcctctg gctcctcatc ctgagcgcgt
     4801 atgcctgcgc gttcctctgg gcctatcgga agcggctgga tcccttctat gacgcggtcg
     4861 cgatggtcgt ggcctgcgtg atggtcttct tcaccagcct gctcaccttc gtctcctcgc
     4921 ccttccgcgt gctggccacg ccgcctcccg acggcaacgg gctgaacccg ctgcttcagg
     4981 atcccggcat gatgatccac ccgcccatcc tctatacggg gtacgtcggg ttcatcatcc
     5041 ccttcgcctt cgggatcgcg gtcctcctca tgaaccgcac cggcacgcgc tggatcgaag
     5101 aggtccgccg ctggacgctc ttcaactgga tgttcctggg cacgggaatc ctcatgggcg
     5161 cccgctgggc ctacatcgag ctgggctggg gcggctactg gggctgggat ccggtcgaga
     5221 acgcctcgct catgccctgg ctggtgggga ccgcgttcct gcactcggtc atgatcgagc
     5281 agcggcgcgg gatgctgaag acctggaacg tggcgctgat cgtgctgacg ttcgagctct
     5341 ccatcttcgg aacgttcctc acccggtcgg gggtgctcac gtcggtgcac tccttcaccg
     5401 agtcgaacat cggcccctgg ttcatgggct tcatcctgat ctccagcacc atcgccgccg
     5461 cgctgatcct ctaccggaaa gcgcttctcg aaagtgagaa ccggctggac gccgtggtga
     5521 gccgcgaggg ctccttcctc ttcaacaacg tgctcttcgt ggcgctctgc ttcgccacct
     5581 tcctcgggac catcttcccg gtggtgagcg aggcggtcac cggctccaag atctccgtct
     5641 cggcgccctt cttcaaccgc gtgaacgtgc cgatcgcgct cgccctgctc cttctgacgg
     5701 gcgcgggccc ggtgctctcc tggaagcggg cgaccgcctc ggtgctgaag cggaacttgg
     5761 tgatcccgac cttcgtcgga gtgctcgccg cggtgatcgg cattcccctc gggctgcacg
     5821 gcctctatcc catcatctgc gtgttcggcg tggcgttcgt ggtctcgacg atcgtgatgg
     5881 agttcgcccg cggcctgcag gcgcggaagg acgtggccga ggccaagggc gcgatggcgg
     5941 tggtcagcct ggtccagaag aacaagcgcc gctacggcgg ctacatcgtg cacgcgggca
     6001 tggtcctggt cttcgtcggc gtgctcggct cgaccgtgtt ccagaaggag gcgcacggcc
     6061 cgctccgcga gggggacaag ctctcggtcg gtccctacgc gctcacgctg cgcggcgtcg
     6121 aggagaagca ggtcgccaac gcccagcaga cgcgcgccgt gctcgacgtg aaccggaacg
     6181 gcaagtcgct cgggctggcg catcccgcga aatcgatgta cttcaaatcg cagcagccga
     6241 tgaccgagat cgcgctgcat cagaccccct ccgaggacct ctacatgatc ctcggcggcg
     6301 tgaacgacga cggctcggtc tcgatccagg cttacatcaa cccgctcgtc agcttcatct
     6361 ggctgggcgg cgtgatcatg gtcttcggga cgatcatcgc gctcaccgac aagatgcggc
     6421 tgcggcgcga ggagaagctg ggcggatgac gcgcgccctc cggctgctcc tcggcgttct
     6481 cgtgggttct gtgctgctct ccggcgcgct gagtgcgccg gcttatgcca agaaggaccc
     6541 tgccaagacc atcacggatg gaatcatctg cccctgctcc tgtggagaga tcctcaccgg
     6601 ctgcacgtgc gagacgggaa aggccatgcg ggcctacgtg gatgatgaag tcaagggagg
     6661 gaagaccaag gatcagatcg aggccgcgct cgtcaatcag tacggcgagg tcattctcgg
     6721 ggcgcccaag gcgaaagggt tcaacctgct cgtctgggcc gcgcccgtgg tggcgaccgt
     6781 gatgggcctc ctgatcgcgt cgttcgtcct cgtccgctgg cgccgccgga atcaggccgc
     6841 ggctcccgcg gcgccacccg cggacggccc ggacgcgccg ttccgcaccg accccgatgc
     6901 ccgcgacgcc gcactgcgcg cgcgggccga agaggagtac cggagactcc aggagtgacg
     6961 gagtcgccac gcctatgatc gctctcgctc aggtctgcct cgccctggcc gtcgccctgt
     7021 tcgtcctctc gccgctctgg ttctaccggg ggcagcgggc gcacatcgag gcggccgacg
     7081 cttccgtccg ccgctcgatc gccgagaaga aggggcgcct ctacagccag ctcctcgacc
     7141 tcgacttcga caaggactcc ggcaagatct ctcccgagga ccacgcccgc atgcgggagg
     7201 agacgatgag cgaggtgctc gcggtgctcg ccgaggagga ccggctgggc ggtccgcggc
     7261 gcgggttgaa gccgaccgtc atcgaagggg gggaccgcgt ggaacggatg atcgaagagt
     7321 acaagcgggc ccgtcccgcg gcgatcgagg tgaaccggtc atgacgaagc agacgctgcg
     7381 cattctcgcg cttccggccg cggtgctgct cctggcgctg gtcctggcgc ttctcgcgat
     7441 cccggcgtgg gcggtcacca tcacggggca cgtgctgaag ggcgccgagc gggccccggt
     7501 gaaggacgct ccggtctcga tccacgtcgt gcgcggcaac gaggagcttc cgggcggcac
     7561 ggtcccctcc gatccggagg gaggcttccg cttcaccggc atcaccgaag gcgcggggct
     7621 cgaatactac ctctccaccg agtacgaggg tgccttctac accgaggggc cgctcgcgat
     7681 ccagaacggc gccgcgtcgc aggacatgca ggtctacgag gtggggaaga acttcgccgc
     7741 ggtgaaggtg caggaccacc acgtgatcgt ggagcggaag gaagacggcc tccacgtcac
     7801 cgagatcctc atcttccaga acgacgcgaa cacggcctac ctgggcgtcg gcgcgaacca
     7861 cgcgatgccc tcgggcatga cggtggggct gcccgcgtcg atcaaggact tcaagccggg
     7921 gctgggactg gacgagcctt cggtgcacgt ccaggggcgc gagatgatgt cgatgcggcc
     7981 gatcccgccg ggacagcggc cgctctcctt cacctacacg attccgctgt ccggacggat
     8041 ggatctctcg caccggctct acttcccgac gcgccagatg acggtgctcc tcgacgatcc
     8101 gaagctgcac gtcgaatcca cccagctcac ggccaacgga acgcgccagc aggggggaaa
     8161 gacctacgcc atgtacatcg gctccgagct tccggtcggc gccgaggtct cgatgagggt
     8221 cggtggcgcg ggcttcttct cgaatccgaa ggtctacccc ttcatggccg cgccgttttt
     8281 gatcgcggcg gcgctgtggt ttgcgtcccg gcgcgggtcg cggcacatga aggagaagca
     8341 catggcggcg caggcggcgg gccatccggc gggtgcgccg tcgggaccgg cttcgcgggc
     8401 tccggtggcg agcaccggtg ggacgggtat cggccccagc gcgggggaaa tcgggaccgc
     8461 ggcctccacg atgagtacgg caggaaagcc tcgccattcg ccttcggcga atggcggaca
     8521 taatgagatg gccgacacgt atctctatct catcgcggcc ctcgaccagg gcgtcgagcg
     8581 cggcgatgtc agcccggaga gccacgcgct gatccgcggc aacctcaagc gtcggctcga
     8641 gacgatcctc gccgacgagc cgcacgcgaa agcccgctga cccacggcgt gatcgcaggg
     8701 gctccgccta tctcgggcgc cgttctccgc gctcttggca tccgaaaacg attcgaatcg
     8761 cgctggatcc tccgggacgc atcgatcgcg ctcgcggccg gtgacgtcgt cctgctcgcc
     8821 ggccggaacg gctccggcaa gaccaccctc gcccgcatcc tcgccacgct gctcgagccc
     8881 gaccagggag aggtcctgt
//
LOCUS       DASSLZ010000153         4672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1573704, whole genome shotgun sequence.
ACCESSION   DASSLZ010000153 DASSLZ010000000
VERSION     DASSLZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573704"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VFM00_05935"
     CDS             <1..1545
                     /locus_tag="VFM00_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HET7497820.1"
                     /translation="WRKIDRLKTGFRGMGNVRLKWRDPKTAFLEGVFARGDVRTGDAL
                     EAAWRLGARFDGWSECFDFDLWMRAFEQVGMDPAVYLRPRDLEETLPWDHIRTPVVKK
                     FLVAERAKAYAAALTPDCRDHACYRCGAPCFTPKAREGRRVSLQLAPGQGKDPDSLAF
                     AVPADITTADIEARVTELKSAPIESMKTPAAPEPAAPAPEPAKPANGNGSGNGYAPAD
                     LRYGRRRRAWPTTAARGTQGTRYRVAFEKMGIARFTSHLDLVRIFDRAIRVAKVPIAY
                     SQGFNRHAKIAYGPPLPLGATSRGEYFDLELAQACPWSAVLAMNDILPEGITITDGRP
                     FVKSSESLMAAIGRADWNVALTGYLMDRLARGEEVHRVRRELEESVARFRAASDWPVT
                     KSSHMQKGRTVNARPAVGGLDLLEDGATLGVRLSSRLQAPGYLRPDLLFKSLLPSFEF
                     DPRLLVVSREAMWVERGTSLLTPLEALEESAFWRPVPADEPPVDATDPNLESNAPRDH
                     HQREQV"
     gene            1508..3061
                     /locus_tag="VFM00_05940"
     CDS             1508..3061
                     /locus_tag="VFM00_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET7497821.1"
                     /translation="MRREIIINASKSETRIALLEEKQLVEVMVERPEAVRRVGDIYKG
                     RVNAVLPGMQAAFVDLGLEKSAFLHASDLTPSESDLDLFEEEEEAEEPRRGGGRGGRR
                     DEPVIRIEDALKKGQEVLVQITKEPIGTKGPRVTTQVSLPGRFLVLMPGHEHIGVSRK
                     IEDRAERARLKTLMREIHPKDAGVIVRTVGAEQGKKEFQSDIRYLEQLWAKIEKQAQR
                     VRAPALLHQEMEFTTGLIRDIFNEDVDQLVIDSPEEHKSILKYLATYAPELRPRVKLY
                     KGEAPIFDHFEIESEIEKAMDRKVWLKKGGYITIDQTEALVAIDVNTGRFTGKKSQEE
                     TILKTNLEAAAEIARQLRLRDLGGIIVLDFIDMEDEANRKAVSDLLRQHLRRDRSRTK
                     SFAVSELGLIEMTRQRQRPSLANYFNEDCPECEGLGKVLSLQSAALKIERMLRRVGQR
                     SREKQLILRVHPDVAVHLVEQNADRLGRLEKQYRYRVEIRDDPSLRRNEIRLFRGRTY
                     EEITKQYER"
     gene            3099..3416
                     /gene="rplU"
                     /locus_tag="VFM00_05945"
     CDS             3099..3416
                     /gene="rplU"
                     /locus_tag="VFM00_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET7497822.1"
                     /translation="MSRYAIIQAQGLQLRVLPGEEHVLPNMDLQPGAPVTFDRVLLIG
                     DEGGVQVGQPLVAGASVTGEVVRSERGKKVTIGVFKRRKKFRKKTGYRDTLTRVRILD
                     IVG"
     gene            3438..3743
                     /gene="rpmA"
                     /locus_tag="VFM00_05950"
     CDS             3438..3743
                     /gene="rpmA"
                     /locus_tag="VFM00_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET7497823.1"
                     /translation="MAHKKGVSSSKNGRDSHSQRLGVKRFGGERVKAGSILVRQRGTV
                     YRPGLLVGRGKDDTLFALATGVVVYRRRGKGNVTVSIEPAEGIPAVAGAQPTEAAHA"
     gene            3736..>4672
                     /gene="mdh"
                     /locus_tag="VFM00_05955"
     CDS             3736..>4672
                     /gene="mdh"
                     /locus_tag="VFM00_05955"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET7497824.1"
                     /translation="MPKITVVGAGNVGASAALYAAEAQLGDVTLIDIIEGVAKGKALD
                     LLEAGPVRHYDSLIEGSGDIRAVAGSDLIIVTAGLPRKPGMSRLDLLKANADIIRGVA
                     EAIRDHAKNAFVILVTNPLDVMAYLTFRVTGFPRERVMGMAGVLDSSRFRTFLAQEIG
                     VSIEEVQAMVLGGHGDTMVPLVRYTTVSGIPVEKFIAKERLAEIVQRTRDGGAEIVKL
                     LQTGSAFYAPAASAVQMAEGILRDKKRLLPVACHLQGEYGFRDIFLGVPAILGSRGLE
                     KIVELELTPEEKSALAKSAEEVKKGIADLSLEPVAH"
BASE COUNT          801 a         1549 c         1664 g          658 t
ORIGIN      
        1 tggcggaaga tcgaccgtct gaagaccggc ttccgcggca tgggcaacgt gcgcctgaaa
       61 tggcgcgatc ccaagaccgc gtttctcgag ggcgtcttcg cgcgcggcga cgtgcgcacg
      121 ggcgacgcgc tggaagcggc atggcggctg ggggcgcggt tcgacgggtg gtcggagtgc
      181 ttcgacttcg atctctggat gcgcgccttc gagcaggtcg gcatggaccc ggccgtctac
      241 ctgcgcccgc gcgacctgga ggagacgctc ccctgggacc acatccgcac ccccgtggtg
      301 aagaagttcc tggtggccga gcgcgccaag gcctacgcgg ccgcgctcac gcccgactgc
      361 cgcgaccacg cctgctaccg ctgcggcgct ccctgcttca cgccgaaagc gcgcgaggga
      421 cggcgcgtct cgctgcagct cgcgccgggc caggggaagg atcccgattc gctcgcgttc
      481 gcggtgccgg ccgatatcac gaccgccgac atcgaggcgc gcgtcaccga gctcaagtcg
      541 gccccgatcg aatcgatgaa gacgcccgcc gcgccggagc cggccgcgcc cgcgccggaa
      601 ccggcgaagc cggcgaacgg gaacgggagc gggaacggct acgccccggc cgatctgcgc
      661 tacggccgcc gccggcgcgc atggcccacg acggccgcgc gcgggacgca gggcacccgt
      721 taccgggtcg cgttcgagaa gatggggatc gcgcgcttca cgtcccatct cgacctggtc
      781 cgcatcttcg accgggcgat ccgcgtggcc aaggtgccga tcgcgtactc gcagggcttc
      841 aaccgccacg ccaagatcgc ctacggaccg ccgctgccgc tgggggcgac gagccgcggg
      901 gaatacttcg acctggagct ggcgcaggcg tgtccctggt cggccgtcct cgccatgaac
      961 gacatcctgc cggaggggat cacgatcacc gacggccgtc cgttcgtgaa gtcgtcggaa
     1021 tccctgatgg ccgccatcgg ccgcgccgac tggaacgtgg cgctcaccgg ctatctcatg
     1081 gaccgcctgg cccggggcga ggaggtgcac cgcgtgcgcc gcgagcttga ggagagcgtg
     1141 gcgcgcttcc gcgctgcctc cgactggccg gtcaccaagt ccagccatat gcagaagggg
     1201 cgcaccgtga acgcgcgccc cgccgtcggg ggcctcgacc tcctggagga cggagcgacc
     1261 ctcggggtcc ggctctcgtc gcggctccag gcgcccggct acctgcgtcc ggaccttcta
     1321 ttcaagagcc tcctgccgtc gttcgaattc gatccgcgcc tcctcgtggt ctcgcgcgag
     1381 gccatgtggg tggagcgcgg cacctcgctc ctcacgcctc tcgaggcgct cgaggagtcg
     1441 gcgttctggc ggcccgtccc cgcggacgaa cccccggtgg acgccacgga ccccaacttg
     1501 gagtcgaatg cgccgcgaga tcatcatcaa cgcgagcaag tctgagacgc gcatcgcgct
     1561 gctcgaagag aaacagctgg tcgaagtcat ggtggaacgg cccgaagccg tccgccgcgt
     1621 gggagacatc tacaaggggc gcgtgaacgc cgtgctcccg ggcatgcagg cggccttcgt
     1681 ggacctgggc ctggagaaga gcgcgttcct gcacgcctcg gatctgacgc catccgagtc
     1741 cgacctcgac ctgttcgagg aagaagagga ggcggaggag ccgcgccgcg gcggcggacg
     1801 cggcggccgg cgcgacgagc cggtgatccg gatcgaggac gccctgaaga aggggcagga
     1861 ggtcctcgtc cagatcacga aggagccgat cggaaccaag gggccccgcg tcacgacgca
     1921 ggtctccctg ccggggcgct tcctcgtgct gatgccgggg cacgagcaca tcggcgtgtc
     1981 gcgcaagatc gaggaccgcg ccgagcgcgc ccgcctgaag acgctgatgc gcgagatcca
     2041 ccccaaggac gcgggcgtga tcgtccgaac cgtcggggcg gagcagggga agaaggagtt
     2101 ccagagcgac atccggtatc tggagcagct ctgggccaag atcgagaagc aggcgcagcg
     2161 ggtgcgcgcg ccggcgctgc tccatcagga gatggaattc acgacggggc tgatccggga
     2221 catcttcaac gaggacgtgg accagctcgt catcgattcg ccggaggagc acaagagcat
     2281 cctgaagtac ctcgcgacct acgcgccgga gctccggccg cgggtcaagc tgtacaaggg
     2341 cgaggcgccg atcttcgacc acttcgagat cgagtcggag atcgagaagg cgatggaccg
     2401 gaaggtgtgg ctcaagaagg ggggctacat cacgatcgac cagaccgagg ccctggtcgc
     2461 gatcgacgtg aacaccgggc gcttcaccgg gaagaagagc caggaagaga ccatcttgaa
     2521 gacgaacctg gaagccgcgg ccgagatcgc gcgccagttg cggcttcggg acctgggggg
     2581 catcatcgtc ctcgacttca tcgacatgga ggacgaggcc aaccgcaagg cggtgtcgga
     2641 cctcctgcgc cagcacctgc gccgggaccg ctcgcggacc aagtcgttcg ccgtgagcga
     2701 gctggggctg atcgagatga cccggcagcg ccagcggccg agcctggcga actacttcaa
     2761 cgaggactgt ccggagtgcg aggggctggg gaaggtcctc tccctgcagt cggccgcgct
     2821 gaagatcgag cggatgctgc gccgcgtggg gcagcgctcg cgggagaagc agctgatcct
     2881 gcgcgtgcac ccggacgtgg cggtccacct ggtggagcag aacgccgacc gattgggccg
     2941 gctggagaag cagtaccggt accgcgtcga gatccgggac gatccgtcgc ttcgccgcaa
     3001 cgagattcgg ctcttccggg gccggacgta cgaggagatc accaagcagt acgaacgctg
     3061 aggtccggcc tcagggagcc aaagggggta ttgacgtcat gagccgttac gcgatcatcc
     3121 aggcacaggg actccagctc cgggtacttc cgggcgagga gcatgtcctg cccaacatgg
     3181 acctccagcc gggggctccg gtcacgtttg accgcgtgct cctgatcggc gacgagggcg
     3241 gcgtgcaggt cggccagccg ctcgtggccg gcgcgtccgt gacgggcgag gtcgtgcgtt
     3301 cggagcgcgg caagaaggtc accatcggcg tgttcaagcg ccgcaagaag ttccgtaaga
     3361 agaccggcta tcgggatacg ctcacgcggg tgcgcatcct cgacatcgtc ggctagccga
     3421 ggacaaagga gagaacgatg gcgcacaaaa agggtgtatc cagctccaag aacggacgcg
     3481 attcgcattc ccagcgactc ggcgtcaaac ggttcggggg cgagcgcgtg aaggcgggga
     3541 gcatcctcgt ccggcagcgc ggcacggtgt accgcccggg gcttctggtc gggcgcggca
     3601 aggacgacac gctgttcgcc ctggcgacgg gcgtggtggt ctaccgccgc cgcggcaagg
     3661 gaaacgtcac ggtttcgatc gagcccgccg agggcatccc ggcggttgcg ggcgcccagc
     3721 ccaccgaggc ggcccatgcc taagatcacc gtggtcggcg cggggaacgt cggcgcgtcg
     3781 gccgcgctct acgcggccga ggcccagctc ggggacgtga ccctgatcga tatcatcgag
     3841 ggcgtcgcga aggggaaggc gctcgacctg ctcgaggccg gtcccgtccg gcactacgac
     3901 tccctgatcg aaggcagcgg cgacatccgc gccgtggccg ggagcgatct gatcatcgtg
     3961 accgccgggc ttccccggaa gccgggcatg tcgcggctgg atctcttgaa ggcgaacgcc
     4021 gacatcatcc gcggcgtggc cgaagcgatc cgcgaccacg cgaagaacgc gttcgtgatc
     4081 ctggtcacga acccgctcga cgtgatggcc tacctcacgt tccgcgtcac cggattcccg
     4141 cgcgagcggg tgatggggat ggcgggcgtg ctcgactcca gccggttccg cacgttcctc
     4201 gcgcaggaga tcggcgtctc gatcgaagag gtgcaggcga tggtgctggg cggccacggc
     4261 gacacgatgg tgccgctggt ccgctacacg accgtctccg gcatccccgt cgagaagttc
     4321 atcgccaagg agcgcctcgc cgagatcgtg cagcgcacgc gcgacggcgg cgccgagatc
     4381 gtgaagctgc tccagacggg cagcgccttc tacgcgccgg cggcctccgc ggtgcagatg
     4441 gccgagggca tcctgcgcga caagaagcgg ctcctcccgg tcgcctgcca ccttcagggc
     4501 gaatacggct tccgcgacat cttcctcggc gtgcccgcca tcttaggctc gcgcggcctg
     4561 gaaaagatcg tggagctgga gctcacgccc gaagagaagt cggcgctcgc caagtcggcg
     4621 gaggaagtga agaagggcat cgcggacctc tcgctcgagc ccgtcgcgca ct
//
LOCUS       DASSLZ010000154        21760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1826097, whole genome shotgun sequence.
ACCESSION   DASSLZ010000154 DASSLZ010000000
VERSION     DASSLZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 21760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21760
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826097"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFM00_05960"
     CDS             <1..303
                     /locus_tag="VFM00_05960"
                     /inference="COORDINATES: protein motif:HMM:NF023785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497825.1"
                     /translation="QMFRAALEYPGFSFVHVLAGCVTYQTPKYADEVYQRCDLLPDSY
                     DPGDLSRAMEVARAERFSLGILYKRPPEAPAATSPWEGEASIWPSTAEANRERPGE"
     gene            complement(420..902)
                     /locus_tag="VFM00_05965"
     CDS             complement(420..902)
                     /locus_tag="VFM00_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497826.1"
                     /translation="MASDREFLRHTLATLAYRGAKATRNAPPEFATFRASPTTRTPVE
                     IVAHLGDLMDWGLSMACGKPAWNNATPQPWPDEIRRFHASMQAFDAYLASDQPLSYSV
                     ERLFQGPIADALSHVGQINTLRRIFGAPVKGESYNRADIQAGRVGPDQTPADSKFEFD
                     "
     gene            1021..1097
                     /locus_tag="VFM00_05970"
     tRNA            1021..1097
                     /locus_tag="VFM00_05970"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1055..1057,aa:Asp,seq:gtc)
     gene            1150..1362
                     /locus_tag="VFM00_05975"
     CDS             1150..1362
                     /locus_tag="VFM00_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497827.1"
                     /translation="MSLTRSRVSRVLAFAAILAVCGATGCTERNMGRADNDGWTKVDT
                     GSKAVQPTAPLQHAEAVNVTYYYLPG"
     gene            1390..1611
                     /locus_tag="VFM00_05980"
     CDS             1390..1611
                     /locus_tag="VFM00_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497828.1"
                     /translation="MSGLEQEFPGKVSARTIEATDPERADEIRGLGFATHGLVIRSKD
                     GAVLFKQPDHTVDISAVEGAIRQILAPKT"
     gene            1813..2367
                     /locus_tag="VFM00_05985"
     CDS             1813..2367
                     /locus_tag="VFM00_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7497829.1"
                     /translation="MNPHEVVLHTDRLILRQFRQDDIDAYAAMHADPDVMRYIGDGKP
                     LSREEAWRSMAAMAGHWILRGYGIWAVEERASGLLAGRVGLYYPDGWPGQEVGWTLAR
                     PFWGKGYAYEAARAAFAYAFDTLRWPRLISLIDPENSRSIALAERLGERLEREVGLRG
                     RRTLQYGIDRPAGLAPAHGGRSGS"
     gene            complement(2362..3462)
                     /gene="rsgA"
                     /locus_tag="VFM00_05990"
     CDS             complement(2362..3462)
                     /gene="rsgA"
                     /locus_tag="VFM00_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET7497830.1"
                     /translation="MTRLESFGWNPFFEQQVPSNTDPEWQPARVYEEQRGSWALWWEG
                     GACRATVAGRLRHESEGEPAALPCVGDWVLARFPGSAAGNGGAAIDGAVIHRVLARKT
                     RFSRQDPGKGTGEQIVAANVDTVFLVQSLNRDFNVRRLERYLALLWESGAEPVVVLSK
                     ADLRPDAAEERAAVERAASGASVHAVSAFTPNGLDPLLPYLAPGRTAALVGSSGVGKS
                     TIVNGLAGEEVMRTQEIRDDDDRGRHTTTSRRIVQLPAGGLLLDTPGMRTVLLWEGEE
                     GLSQTFGDVESVAAGCRFRDCTHQTEPGCAVRAALEDGSLDSGRFASYGKLQREVNYQ
                     ARKTDLRLRLAEQRRWKKIHLAARQRPDKRSL"
     gene            complement(3804..4946)
                     /locus_tag="VFM00_05995"
     CDS             complement(3804..4946)
                     /locus_tag="VFM00_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7497831.1"
                     /translation="MTSIPEITPEELVQRLESGAALRVLDVRTPEAVAAGRIDLLPSD
                     RFVNVRGSELLALRGAVKEKLPPDGPIAVVCGHGNSSKQVAQFLNHLGYDAMSMRGGM
                     AAWDLAVMPRKLAPPAGFDHFVQFDRVAKGATGYLLASKGEALLVDPPRKAQPYLDHA
                     RELGCRVVAVADTHAHADYISGGPALARSLQIPYYLHPGDAVYPFDGRPAAIPFSPIE
                     EGSAIRVGHTEACVRHTPGHTEGSVTFMAGDAALTGDFIFVNSVGRPDLGGKSDAWTP
                     VLWDSLERARREWDPATRVYPAHYASPSEREPDQSVGRPFGRLLESNVPLAMKDRDAF
                     LSWVGAKVGKAPDAYKKIKAVNLGLLEVWDMEAQQLEGGRNECALG"
     gene            5037..6140
                     /locus_tag="VFM00_06000"
     CDS             5037..6140
                     /locus_tag="VFM00_06000"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HET7497832.1"
                     /translation="MPSHVRTMHTTGTGVLPGLALSLLVALLARLLHQAVPHGVAAAV
                     GEVVLAVLLGLLIGNIIGLPPLFAPGVRFSFHAVLRMAIVLLGASFSVQQVASIGGRA
                     VIMVVVLMTIALIAAHALGKAARVPPRLATLIGVGTAVCGNSAIVATAPVIGAHDDEV
                     SFAIATNTLFGTLAVFLYPLIGHALHLRDPAFGTWAGSAVNDTSQVVATGFAYSDGAG
                     RIATAVKLTRNALMGGVIVLMGLLHGGARHDVEGSGATSAGAPARGGFWLRVKQSIPL
                     FVLGFLAMAALQSAGVLRWLSIRAGRDLSADAQNVARVLILIALAGVGLSTRIASMRR
                     AGLRPFYVGLVVATAVSAVSLLWIHTVGPAGGS"
     gene            6137..7141
                     /locus_tag="VFM00_06005"
     CDS             6137..7141
                     /locus_tag="VFM00_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7497833.1"
                     /translation="MTPRANKGGSPRLPELPKGIYVRRPRQEDGPEIAALMNAVDTAD
                     IGVAENTVEEVYETWALPRFQKERDAWIVLEGTGKVVGYAFAWDRKPHLEIQADSYVA
                     PALAASGLEEALLDRIEERAREHRAAAPKSEEVLIRGFTSPTGKERIAAFLSRGYAHV
                     RTFCRMTIDLGAPDTIPAARWPAGIEPRGYVRGRDEKAFDRAIQESFADHFGFVLEPH
                     EEWIARRVDQSGFDAGLWTLALEGKEPAGAILAYAPDGEGWVRELGVRPAWRGRGLGR
                     ALLLHTFTLFARRGIQRVGLGVDMANATGATQLYESVGMRVAFRHDLYEKRLGPGLAS
                     "
     gene            7314..7544
                     /locus_tag="VFM00_06010"
     CDS             7314..7544
                     /locus_tag="VFM00_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497834.1"
                     /translation="MAASGSELFEPIARRRSDIFDRDPFDDDEEENLENGEFDDEDEL
                     DEDEEEDEDEFEDDDYDDDDDLEEDEEEEDDL"
     gene            7625..8569
                     /gene="speB"
                     /locus_tag="VFM00_06015"
     CDS             7625..8569
                     /gene="speB"
                     /locus_tag="VFM00_06015"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HET7497835.1"
                     /translation="MPRPSRMKLASPAPALFLGLPPESCRIERARVAVIPAPFDQTTS
                     YLPGTRFGPAAILSASRQVEFYDEELDREPCDIGIATLEEIEVEPADIESGLRRLEQV
                     VESLDERGIVPLTLGGEHSLTLAPVAALRRRYPELSVIQLDAHLDLRDAYQGTKLSHA
                     SVMRRIRELGVPTVPVGIRSVSREEADYVHAEKAPVFLAREIRAGGLDVEAILGELGN
                     PVYVTVDLDAFDPAYVPGVGTPEPGGLTWDEGLRLLRAVFERRQVVGIDVVELCPIPG
                     QIVSDFFAAKLVNKLIGYLGLAPNAVSARRRRPKSPRG"
     gene            8575..9756
                     /locus_tag="VFM00_06020"
     CDS             8575..9756
                     /locus_tag="VFM00_06020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET7497836.1"
                     /translation="MPAAVLRLAKNQERRIRAGHPWVFSNEIESVEGSPESGGEVLVH
                     DHRGGVVGVGLYNPNSLIAVRLFARRERPIDADLFRERIRAARALRDRIYPVETTYRL
                     IYSEGDFLPGLIVDRYGDYLGVQSLTAGIERRLDEVLDALQEVMQPAGIVCRRDAPAR
                     SYENLPLMEPVERGVVPERVDAPFQGFVLTVDLRTGQKTGEFLDQRENRQRVARESVG
                     RRVLDLYCYTGLFSLHCAAAGATSVLGVDRSAPAVERARENARRNAPSRDVEFRAEEV
                     ERYVRDAVAAEERFGMIILDPPALVKSRKALQEGLRKYAQLNAAALRLLEPGGVLATA
                     TCSHHVDAPLFLDVLRSAAKDAGQPLRVVDVLGQSRDHPVLLAARETSYLTMVIAERI
                     H"
     gene            9764..10111
                     /locus_tag="VFM00_06025"
     CDS             9764..10111
                     /locus_tag="VFM00_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HET7497837.1"
                     /translation="MNDNAESTPSRKDLFLGLVHSFQAAAMQQMGKIMNPFTEKMERD
                     LKQARLSIDMLEMLQERTSGNLTGEEARFLTHVLTELRLNYVAELDEDKKLAAQPSNE
                     PAPGVSGPAEPGA"
     gene            10247..13657
                     /locus_tag="VFM00_06030"
     CDS             10247..13657
                     /locus_tag="VFM00_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7497838.1"
                     /translation="MNDKDVHAPLNLPGGGEMAALIRSIDWSSTPLGPVSSWSPTLRM
                     MVPFLVANRFPLLLWWGPDYCQIYNDAYRPILGSKHPRSMGQPTRECWTEIWDVLGPL
                     IDTPYRGGPPTWMEDLALEINRHGFLEETHFTVAYSPVPDDTAPGGIGGVLATVHEIS
                     EKVVGERRVRALRDLAAGGPEAKTVEEACSAAGKTIERYPKDIPFALVYLVEPDGARA
                     TLACAVGIEEDGPASPRTIDLLSERAAWPLGLALRTDATQLVEDLAARFGAAVPPGPW
                     ADPPRQAAVMPVRSTMARKYAGFLVAGISPRLRFDESYRSFFELATGQIATAVANARA
                     YDEERRRAEALAAIDRAKTAFFSNVSHEFRTPLTLMLGPLEDALERGALPDEERERLE
                     LIHRNALRLSKLVNALLDFSRIEAGRAQATYRPTDLGTLTADLASAFRSAIERAGLEL
                     IVRCPSVGEPAWVDPAMWEKIVLNLLSNAFKFTLQGTIEISLETVDREWRLTVRDTGI
                     GIEAGDRARIFDRFHRVEGARGRSYEGSGIGLALVLELARLHGGSARVESAVGQGSAF
                     VVHIPRGHAHLPPERVVAGEASRPGLERAFVEEALRWLPDSDADRLGSSAPDANAPIR
                     GGRARAPLPRVLLADDNADLRDYVRRLLEPHYEVETVPDGEAALESIHRRPPDLVLSD
                     VMMPRRDGFALLRALREDPDTASLPVILLSARAGEESRVEGLELGADDYLVKPFAARE
                     LLARVASRIVDSERRASESRVRYVLESVSDGFQVLDAEGRYRYANLAMRRLWEGFGRS
                     SEVIGRHVFEVFPEARETTLGRALQRALTERVPIEIETFYEPFQRWFMVRYYPSPDGG
                     LSIVSQDITARKLSEDRAREAERRKDEFLATLAHELRNPLAPIRNAIAILQSRGTADL
                     EARWARGVIERQVASMARLLDDLLDISRIAKNLLQLRKDRHTLAEIVQRSLETSQPAI
                     QAADHSLAVSLPEEDLWLEADPLRLAQVLSNLLNNAAKYTPRGGRIELSGRREGSDVV
                     IGVRDNGIGIAPDFLPQVFEMFVQGAPAADVSEGGLGIGLSLVQGIVTAHGGRVHARS
                     GGPGQGSEFTVRLPAAPPRSDEVAVPAAQLDRESIARTKAVTSEDATV"
     gene            complement(13654..14493)
                     /gene="mazG"
                     /locus_tag="VFM00_06035"
     CDS             complement(13654..14493)
                     /gene="mazG"
                     /locus_tag="VFM00_06035"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET7497839.1"
                     /translation="MDAPRPPLSPPARDRSDFAAAAAELLDLVRHLASDEGCPWDREQ
                     TPRTLTPYVVEEAHEIGEAVAEANADGASEELGDLFYLACFLAVALEREGGATPAAVL
                     RRNIAKMIGRHPHVFGDEAAEPELDAQGVLRRWEERKRKESADASILGKRPDALPALL
                     QAYRIQEKAASVGFDWEHAQGVVEKIREELAEVEREMDLAAPERIAEEIGDLLFAVVN
                     LSRFLRIDPEAHLRLATEKFRGRFDRVAAMLREQGRSVDEAGLPELDRLWEAAKREER
                     ERD"
     gene            14601..15848
                     /gene="alr"
                     /locus_tag="VFM00_06040"
     CDS             14601..15848
                     /gene="alr"
                     /locus_tag="VFM00_06040"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7497840.1"
                     /translation="MEFPTWVEVDLNRFRRNLAAIATAIGPRRRILLVVKADAYGHGA
                     VEIARCAVQNGVSMLGVATLHEGIELRSAGIDLPVVILSPCLLSEVDEIIEHRLTPCV
                     ATLGFAQRLSERCAAHQVLCRVHVEVDTGMGRAGVAESEAVDFVERLTSLPNLRLEGI
                     FTHFPDADSGRTDVSEEQLRRFGAVLHALELRKIEVPVRHAANSAGILSVHDSYLDMV
                     RPGILAYGFYPSRAVPHSVEVEGVMAFKTRIVQLRDLPPGRFVSYGRTFQAPGWMRIA
                     VLAVGYGHGYPWALSNRGEVLIRGRRAPVVGRVTMDLTMVDVSAIPEAMPEDEVVLWG
                     EQGGARLDLGEVAERAQTIPYDLLCSMGKRVVRLYHNDGEPMKALTLIGVREETEVIE
                     GGGAAGPRRRRRRVRYRSTRSGS"
     gene            15871..16257
                     /gene="cdd"
                     /locus_tag="VFM00_06045"
     CDS             15871..16257
                     /gene="cdd"
                     /locus_tag="VFM00_06045"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET7497841.1"
                     /translation="MSDGVKLLEAARVLLAKAHAPYSHYRVAAALEDEHGVVHPGVNV
                     ESASLGLTICAERNALFGALARGARRFRRIAIATDPPGPCVPCGACRQVLVEHAPDLE
                     VIVASPEGAPEMIPLRDLLPRPFLTF"
     gene            16257..17552
                     /locus_tag="VFM00_06050"
     CDS             16257..17552
                     /locus_tag="VFM00_06050"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET7497842.1"
                     /translation="MRDVIAAKRDGRALSEAQIRAWIRGVTDGSIPDYQSAALLMAIV
                     WRGMSRDETAVLTDALMRSGRVLDWSGLGRPTVDKHSTGGVGDKVSIALAPWVAACGA
                     VVPMIAGRGLGHTGGTLDKLEAIPGFRTRLSAAEFEGQVRKIGVAIAGQGEDLAPADG
                     ALYALRDVTGTVESVPLIVASILSKKAASGAAGVVFDVKCGAGAFMPGREEAAALARE
                     LVAVLSGMGRKAHALVTAMDEPLGAAVGNANETAEAFAVLHRAAPADLMDVTQALAVR
                     MLVLSGVARERHEAEARLDRALETGEALGRAERMVQAQGGDPRVVGDPGRLPRAPVEM
                     AVPALRAGYVGAVDARRIGELVIAMGGGRRRKEDVVDPAVGVRLLRKGGERVDAGEVL
                     ALIQARRDAPEWTEAAARAFTIADEPPDKSPLVLEEIAP"
     gene            17549..18157
                     /locus_tag="VFM00_06055"
     CDS             17549..18157
                     /locus_tag="VFM00_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7497843.1"
                     /translation="MKRASRGRSAPSRGDLRVDPATPDRWDDLAALFGPRGACAGCWC
                     MWWRRSAAEWKRGKGDGNRRALRRLVDSGEPPGLIAYDGETPIGWIALAPREAYARLA
                     SARSLKPVDDTPVWSVTCFFVTKDYRRRGVSAHLLRAAAEHARKRGAKMIEGYPVVSR
                     AGRMPDAFAWTGLPSAFERAGFREVARPSASRRIMRKALKKS"
     gene            complement(18158..20275)
                     /locus_tag="VFM00_06060"
     CDS             complement(18158..20275)
                     /locus_tag="VFM00_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET7497844.1"
                     /translation="MSHRWKWIAQFTAALLIVVLCATVGVGIGVANWARQDLPSPSSL
                     QTIAPPVKTLVYDVNGKLVHEFYKENRSVVPLKQIPKPMVDAILSIEDRRFYSHWGID
                     PVRLFGALVQDVIARRPEQGASTITQQLARNLFLTHEKTVSRKIKEAILAVRIEQTYT
                     KDEILQMYLNQIYFGEGAYGVDAAARIYFGKSVQELTLPECALLAGLPRNPRDYAPRR
                     DPDRALKRRNLVLAAMVQNKKLSQKQYEAASETPLGVSKTRIDTKSAPYYMEMVRLYL
                     EERYGSNQLFEGGLRVYTTLDADLQHAAEESLERRLTELEQRIGGKRSRAAILAKAKT
                     AQPSERTQTDYLQGAVVAIDPTNGQIRALIGGRDFNESNFNRATQAARQPGSAFKPFI
                     YTAAIDNGYTPNEMILDTPVSFKAGNGEDWSPQNYDHKFRGPVTLRTALAKSLNVPAA
                     KLLQKIGTPQVISYARRLGIRSRLGSDLSLALGTSEVNLLELTSSYGVFANQGVRTTP
                     VFVLRVEDKNGKVLEQTRTTAEEVLSPETALTMTNMMETVLQSGTAAAAHAMGLNVPA
                     AGKTGTTDDYTDAWFVGYTPSLVTGVWVGFDRKQKIGPNMTGAAAALPIWIDVVSAGT
                     KNKPAQDFPVPNGVVARLICSQTGLLANPACPETEMELFKEGTEPTGYCDIHTGATPP
                     PESEPRDLEQHDSEAQPEEHLRL"
     gene            20392..21636
                     /gene="tyrS"
                     /locus_tag="VFM00_06065"
     CDS             20392..21636
                     /gene="tyrS"
                     /locus_tag="VFM00_06065"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET7497845.1"
                     /translation="MNDKPILSAEAARQLAVIRSGVEELIPEAELVKKLERSLATKTP
                     LRVKQGFDPTAPDIHLGHTIGLRKLRQFQELGHQVVLIVGSYTALVGDPSGRNKTRPR
                     LTEAEVEANAQTYLAQFFKVVDKSKTEVRWNGEWFSAMRFTDVLHLTSQFTVARLLER
                     DDFDQRFKAGEPIGVHELFYPLMQAYDSVVIRADVEIGATEQKFNLLTGRDIQMAYGV
                     EPQVILTLPVLEGLDGVRRMSKSLGNYIGVSESPKEIFGKAMSLPDSMIERYFRLVTD
                     VSDQECAEVAKSLRDPSVNPMVWKKALAHQLVRQYHGHEEADRARDDFEKQFSRREIP
                     SEMPVVTVESGRFRARDLVMRAFPNQYTGTAAGNLFRQGAVHIGGERVTDIAREFDLR
                     KGSETEELVIKVGRKFAKVVAA"
BASE COUNT         3217 a         7421 c         7649 g         3473 t
ORIGIN      
        1 cagatgttcc gcgcggcgct ggaatatccc ggattctcgt tcgtgcacgt gctggcggga
       61 tgcgtgacct accagacccc caagtacgcc gacgaggtct atcagcgttg cgatctgctc
      121 cccgacagct acgacccggg cgacttgagc cgggcgatgg aggtggcgcg cgcggagcgc
      181 ttctcgctgg ggattctcta caaacgcccg cccgaggccc cggccgcgac gtcgccgtgg
      241 gagggagagg cgagcatctg gccgtcgacg gcggaggcga atcgggagcg cccgggggag
      301 taggcgccag aaaacgagac agttaacata taactaaact tgcgccgggt tgctcggact
      361 cgccccgggg cgagttgggt tatgtcaact ccacgtcagc ttccgttacc tgttcgggat
      421 caatcgaact cgaacttcga gtcggccggg gtctgatcgg gtccgactcg tcccgcctgg
      481 atgtccgcgc ggttataact ctcgcccttg accggcgcgc cgaagatccg ccgcagcgtg
      541 ttgatctggc ccacgtggga gagcgcgtcc gcgatcggtc cctggaagag gcgttccacg
      601 gagtagctca agggctggtc cgacgcgagg taggcgtcga acgcctgcat ggacgcatgg
      661 aagcgccgga tctcgtccgg ccacggctgc ggcgtggcgt tgttccacgc cggcttccca
      721 cacgccatcg aaagccccca gtccatcaga tccccgaggt gagccacgat ctcgaccgga
      781 gttcgggtcg tgggagaggc gcggaacgtc gcgaactcgg gaggagcgtt ccgggtcgcc
      841 ttcgctccgc gataggccag cgtggccagg gtgtggcgta gaaactcgcg atccgatgcc
      901 atggcgtctc ctgggttcag aagtggcgtg gctcagcctg gaagggttct ctctgcccgt
      961 gtttgaccgc tgccgccgca acccttatac tcccacgtct tgcttcggct ttgcattggt
     1021 ggggtcgtag ttcagttggt tagaacgcct gcctgtcacg caggaggtcg cgggttcgag
     1081 tcccgtcgac cccgccattt tgtgcacgta acttcaagcg ctgctcccca ctagacccat
     1141 ggaaggggaa tgagcctgac gagatcccgg gtatccagag tcctcgcgtt cgccgcgatc
     1201 ctcgcggtgt gcggcgccac gggctgcacc gaacgcaaca tgggccgcgc cgacaacgac
     1261 ggctggacca aggtggatac cggctccaag gccgtccagc ccacagcacc cctgcagcac
     1321 gccgaggcgg tcaacgtcac gtactactac cttcccggct gaatggtctg cgcgcggttg
     1381 agacccgccg tgagcgggct cgagcaggag ttcccgggga aggtgagcgc acgcaccatc
     1441 gaagccaccg accccgagcg cgcggacgag atccgcggcc tgggattcgc gacgcacggc
     1501 ctggtgatcc gctccaagga cggcgccgtc ctcttcaagc agccggacca cacggtcgac
     1561 atctcggccg tggagggcgc catccggcag atcctcgccc cgaagacctg aatcgccggc
     1621 gcaccgccgc acgtctgtga aacggttcgc cgcgccacac gaccgcggat tctatcttct
     1681 cccggcgcga gtccggccgc tcgtgaattc ccttcaagtt gacctaatta cttgcgctgg
     1741 cgcaggtaac atttatgtta atcgcgctag tgcggagtag atcatgcgct tacacgaaat
     1801 cgggtagaga ccatgaatcc gcatgaagtc gtcctccaca ccgaccggct gatcctccga
     1861 cagttccgcc aggacgacat cgacgcgtac gcggcgatgc acgccgatcc cgacgtcatg
     1921 cgctacatcg gcgacgggaa accgctctca cgcgaggagg cgtggcggag catggccgcc
     1981 atggcggggc actggatctt gcgaggctac ggcatctggg cggtcgagga gcgcgccagc
     2041 ggcctcctcg cggggcgcgt cggcctctac taccccgatg gctggccggg gcaggaagtc
     2101 ggctggaccc tggcgcgccc gttctgggga aagggctatg cttacgaagc cgcccgcgcc
     2161 gcgttcgcct atgccttcga tacgctccgg tggccgcgcc tcatcagcct gatcgacccc
     2221 gagaacagcc ggtcgatcgc gctcgcggag cggctggggg agcggctcga gcgggaggtg
     2281 gggctccggg gccggcgcac gctgcagtac ggcatcgacc gccccgccgg cctcgcgccg
     2341 gcgcacggag gccgctccgg ctcatagcga cctcttgtcg gggcgctgcc gggctgccag
     2401 gtggatcttc ttccaccgcc gctgctcggc cagccggagc ctcaggtcgg tcttccgagc
     2461 ctggtagttg acctcccgct gcagcttgcc gtagctcgcg aagcggcccg aatcgagcga
     2521 gccgtcttcc aacgcggcgc gcacggcgca tcccggctcg gtctggtggg tgcagtcgcg
     2581 gaaccggcag cccgccgcga ccgattccac gtccccgaac gtctgcgaca gaccctcttc
     2641 tccctcccag agcagcacgg tgcgcatccc cggcgtgtcg agcaacagtc cccctgccgg
     2701 tagctgaacg atcctccgcg acgtcgtggt gtgccggccg cggtcgtcgt cgtcgcggat
     2761 ctcctgggtg cgcatcacct cttcgccggc gagtccgttc acgatcgtgg actttccgac
     2821 gccggacgat ccgacgagcg ccgccgtgcg gcccggcgcg agatagggaa gcagcggatc
     2881 gagtccgttc ggcgtgaagg cgctcaccgc gtggaccgac gcgcccgaag cggcgcgctc
     2941 cacagcggcc cgctcctccg ccgcgtccgg acggagatcg gccttgctca agacgacgac
     3001 cggctcggcg ccactctccc acaggagggc gagatagcgc tccagccgcc gcacgttgaa
     3061 atcgcgattc agcgactgca ccagaaacac ggtgtccacg ttggccgcga cgatctgttc
     3121 gcccgttccc ttgcccggat cctgccggga gaagcgggtt ttccgcgcga gcacgcgatg
     3181 gatcacggcc ccgtcgatcg cggcgcctcc attcccggcc gcggagcccg ggaaccgcgc
     3241 cagcacccag tcgccgacgc aggggagagc ggccggctcc ccctcggatt catggcgcag
     3301 gcgtcccgcc acggtcgcgc ggcacgctcc tccctcccac cacagcgccc agctcccgcg
     3361 ctgctcttcg tagacgcggg cgggttgcca ctcgggatcg gtattcgatg gcacctgctg
     3421 ctcgaagaac ggattccagc caaacgattc cagtcgggtc acgacggtct tcctccggat
     3481 gaatgggaat gcccatgtcc ggttcctcgc ccaggccggt gaccgcgcat cgcgcaaagc
     3541 gcgggcaagg ccctgcagac gcaggaacac cctgcgaacg cagcgacccc gcgggcccac
     3601 gaaggcgcgc gcgatcggtc agcgagattg gggcgaagga accggtggag cggttgggcc
     3661 ggccagcggc ccgctacggc ctaacggccg gacacagctc cgagggcggc aatggctagg
     3721 ttcgtcatgg tcatgccctc catcgttgag gaagactggg ttgcggtggt gctgcgacga
     3781 cgacgaagat tatatcagaa acgtcacccg agggcgcact cgttacggcc gccctcgagc
     3841 tgctgcgcct ccatgtccca cacttccagg aggcccaggt tcaccgcctt gatcttcttg
     3901 taggcgtccg gcgccttgcc caccttggcg ccgacccacg agagaaacgc gtcccgatcc
     3961 ttcatggcga gcgggacgtt gctctcgaga aggcgcccga acggccggcc gacgctctgg
     4021 tccggctcgc gctcgctcgg cgaggcgtag tgcgcggggt agacgcgggt cgcggggtcc
     4081 cactcgcggc gcgcgcgttc caggctgtcc cacagcaccg gcgtccacgc gtcggacttg
     4141 cctcccaggt cggggcgccc caccgaattc acgaagatga agtcgcccgt gagggcggcg
     4201 tcgccggcca tgaaggtgac gctcccctcg gtatggcccg gcgtgtggcg gacgcaggcc
     4261 tcggtatggc ccacccggat cgccgagccc tcttcgatcg gcgagaaggg aatggcggcg
     4321 ggtcttccgt cgaagggata cacggcgtcg ccgggatgca ggtaataggg aatctggagc
     4381 gagcgcgcca gcgcggggcc gccgctgatg tagtcggcgt gtgcgtgcgt gtcggcgacc
     4441 gcgaccacgc ggcatcccag ctcgcgcgcg tgatccaggt agggctgcgc cttgcgcggg
     4501 ggatcgacca gcagcgcctc tcccttcgat gcgagcaggt atcccgtcgc gcccttggcc
     4561 acgcggtcga actgcacgaa gtgatcgaac ccggccggcg gcgccagctt ccgcggcatc
     4621 accgccagat cccacgcggc catgccgccg cgcatcgaca tcgcgtcgta gcccaggtga
     4681 ttcaggaact gcgccacctg cttgctgctg ttcccatgcc cgcagaccac ggcgatcgga
     4741 ccgtcgggcg gaagcttctc cttcaccgct ccgcgcaggg cgagcagctc ggagccgcgg
     4801 acgttcacga accggtcgga agggagcagg tcgatccgtc cggccgcgac cgcctcgggc
     4861 gtgcggacgt ccaggacgcg cagcgcggct cccgattcca gccgctgcac cagttcttcg
     4921 ggggtgatct cggggatgga ggtcatggat gcagtgtggc gaacgggcgt gggtcgcgtc
     4981 aaggctcctg gccagaacgc ttgtcgcgcc ggtcgggacc ggtcaagatg cgccccatgc
     5041 cgtctcacgt ccgaaccatg cacacgaccg ggaccggcgt ccttccgggg ctcgcgctca
     5101 gcctcctggt cgcactgctg gcgcgcctgc tccaccaggc ggtcccgcat ggcgtggccg
     5161 ccgcggtcgg cgaggtggtc ctcgccgtgc tcctcgggct cctgatcggc aacatcatcg
     5221 gactcccacc gctctttgcg cccggggtcc gcttctcgtt ccacgccgtt ctgcggatgg
     5281 ccatcgtgct cctgggtgcc tccttctcgg tgcagcaggt cgcctcgatc ggggggcgcg
     5341 ccgtgatcat ggtcgtcgtc ctcatgacga tcgcgctgat cgcggcccat gcgctgggaa
     5401 aggcggcgcg cgtgccgccg cggctggcca ccctgatcgg cgtcggaacc gcggtctgcg
     5461 gcaactcggc gatcgtcgcg acggcgccgg tgatcggggc ccacgacgac gaggtctcct
     5521 tcgccatcgc gacgaacacg ctcttcggga cgctggccgt gttcctctat ccgctgatcg
     5581 gccacgcgct gcacctgcgc gatcccgcgt tcggaacctg ggccggctcc gccgtgaacg
     5641 acacctcgca ggtggtcgcg accggcttcg cctacagcga cggagcggga aggatcgcca
     5701 ccgcggtcaa gctgacgcgg aacgcgctca tgggcggggt gatcgtgctg atgggtctgc
     5761 tgcatggcgg ggcgcggcat gacgtcgagg gaagcggcgc gacctcggcg ggggccccgg
     5821 cgcgcggggg gttctggctc cgcgtgaagc agtcgatccc gctcttcgtc ctcggcttcc
     5881 tggccatggc ggcgctgcag tcggcgggcg tgttgcgctg gctttccatc cgcgccggtc
     5941 gtgacctgtc cgccgacgcc cagaacgtgg cccgcgtcct gatcctgatc gccctggccg
     6001 gggtggggct ctcgaccagg atcgcgtcga tgcgccgcgc ggggcttcgc cccttctacg
     6061 tgggcctggt cgtggcgacc gccgtgtccg ccgtgagcct tctctggatc cacaccgtcg
     6121 gtccggcggg aggatcatga ccccgcgcgc gaacaagggc gggagcccgc ggcttcccga
     6181 gcttccgaag ggaatctacg tccggcgtcc gaggcaggag gacggccccg agatcgccgc
     6241 gctcatgaac gccgtggaca cggcggacat cggcgtggcc gagaacaccg tcgaggaggt
     6301 gtacgagact tgggcgctcc cccgattcca gaaggaacgg gacgcctgga tcgtgctgga
     6361 ggggaccggg aaggtcgtcg gctatgcgtt cgcctgggac cggaagccgc acctcgagat
     6421 ccaggccgat tcctacgtcg caccggcgct cgcggcgagc gggctggagg aggcactcct
     6481 cgaccggatc gaagagcgcg cccgggagca ccgcgcggcg gcgcccaagt ccgaagaggt
     6541 tctgatccgc ggcttcacct ccccgaccgg gaaggagcgg atcgcggcgt tcctctcgcg
     6601 ggggtatgcc cacgtccgga ccttctgccg gatgaccatc gacctgggcg cccccgacac
     6661 gattcccgcc gcccgatggc ccgccggcat cgagccgcgc ggctacgttc ggggacggga
     6721 cgagaaggcc ttcgaccggg ccatccagga gtcgttcgcc gaccacttcg gcttcgtgct
     6781 cgagccgcac gaggagtgga tcgcccgccg cgtggaccag tcgggcttcg acgccgggct
     6841 ctggacgctg gcgctggagg ggaaggagcc tgcgggagcg atccttgcgt acgcgccgga
     6901 cggtgaagga tgggtgcggg aactgggtgt ccggccggcg tggagagggc ggggactggg
     6961 gcgcgcgctc ctcctgcata cgttcaccct tttcgcgcgg cgcggcatcc aacgggtcgg
     7021 actcggggtc gacatggcga acgcaacggg ggcgacgcaa ttgtacgaat cggttggaat
     7081 gcgtgtcgcg ttccgtcacg atctctacga gaagcggctc ggccccggac tggcttcgta
     7141 acccattgcg cgtccggaag tagcgcgtga gacaaaaatg gctcaccctg ccgcgtcgtt
     7201 ttccgttgac ggcccacgtg ggggtactta atttcccgac gcggtttttt tcgatggcgc
     7261 ccgccgctcg atgaaatgga ccgggattga gggattgcgc gatgacgtgg tcaatggctg
     7321 catccggaag cgagttgttc gagccgatcg cccggcgccg ctcggacatt ttcgatcggg
     7381 acccgttcga cgacgacgag gaagagaatc tcgagaacgg cgaattcgat gacgaggacg
     7441 agctcgacga ggacgaggaa gaagacgagg acgagttcga ggacgacgac tacgacgacg
     7501 acgacgacct cgaggaggac gaggaggagg aggacgatct ctagttcggc ttcgcccttc
     7561 caggtcacga cgccttgatc cgcgcggcgg ctcgccgcgc ggatttccat tccgggggga
     7621 ttccgtgccg cgtccaagcc gcatgaagct ggcctcgccc gcgcccgcgc tgttcctggg
     7681 ccttcctccg gaatcctgcc ggatcgaacg cgcgcgcgtg gccgtgatcc ccgcgccctt
     7741 cgaccagacc acgagctacc tgcccggcac ccgcttcggc ccggccgcca tcctgagcgc
     7801 ctcgcgccag gtggagttct acgacgaaga actggaccgc gaaccctgcg acatcggcat
     7861 cgccacgctc gaggagatcg aggtcgagcc cgccgacatc gagtcgggac tccgtcgcct
     7921 ggaacaggtc gtggaatcgc tggacgagcg ggggatcgtg ccgctcacgc tggggggcga
     7981 gcactcgctc accctggcgc cggtggccgc gctacgccgc cgctatcccg agctctcggt
     8041 gatccaactg gacgcgcacc tcgacctgcg cgacgcctac cagggaacga agctgagcca
     8101 cgcgagcgtc atgcgccgga tccgggagct cggcgtcccc acggtcccgg tcgggatccg
     8161 gagcgtctcc cgcgaggaag ccgactacgt gcacgccgag aaggcgcccg tcttcctggc
     8221 gcgtgagatc cgcgccgggg gcctggacgt ggaggccatc ctgggggagc tgggcaatcc
     8281 ggtctacgtc accgtggatc tcgacgcctt cgatccggcc tacgttccgg gagtcggcac
     8341 gcccgagccg ggcggcctca cgtgggacga ggggcttcgc ctgctccgcg ccgtgttcga
     8401 gcggcgacag gtggtcggca tcgacgtggt cgagctgtgc cccatccccg ggcagatcgt
     8461 ctccgatttc ttcgcggcga agctggtgaa caagctgatc ggctatctcg ggctcgctcc
     8521 gaacgccgtc tcggcgcgcc gccgccgtcc caagtctccg cgcggctgac cgtcatgccc
     8581 gccgccgtgc tccgcctggc caagaaccag gagcggcgca tccgggccgg gcatccctgg
     8641 gtcttcagca acgagatcga atcggtcgag ggctcgcccg aatcgggggg cgaggtgctc
     8701 gttcacgatc accgcggcgg ggtcgtcggc gtggggctct acaacccgaa ttccctgatc
     8761 gcggtgcggc tcttcgcgcg gcgcgagcgt cccatcgacg ccgacctctt ccgcgagcgg
     8821 atccgcgccg cgcgcgcgct ccgcgaccgc atctaccccg tcgagacgac ataccggctg
     8881 atctacagcg aaggggattt cctgcccggg ctgatcgtgg accgctacgg cgactatctg
     8941 ggcgtgcaga gtctgaccgc cgggatcgag cggcggctgg acgaggtgct ggacgcgctc
     9001 caggaggtga tgcagcccgc gggcatcgtc tgccggcgcg acgcgccggc gcgctcctac
     9061 gagaacctgc cgctgatgga gcccgtcgaa cggggggtcg tgcccgaacg cgtggacgcg
     9121 cccttccagg ggttcgtcct gacggtcgat cttcgcaccg gacagaagac gggggagttc
     9181 ctggaccagc gggaaaaccg ccagcgcgtg gcccgggaat cggtcgggcg ccgcgtgctc
     9241 gatctctact gctacacggg gctcttttcg ctccactgcg ccgccgccgg cgcgacgagc
     9301 gtcctgggcg tggaccgctc ggctcccgcg gtggagcgcg cccgggagaa tgcgcggcgc
     9361 aacgccccct cgcgcgacgt ggagttccgg gccgaggagg tggagcgcta cgtgcgcgac
     9421 gcggtcgccg ccgaggagcg ctttggtatg atcatcctcg acccgccggc cctggtgaaa
     9481 agccggaagg cgctccagga ggggctccgg aagtacgcgc agctgaacgc cgccgcgctc
     9541 cggctcctcg agccgggcgg ggtgctcgcg accgcgacct gctcgcatca cgtcgatgcg
     9601 ccgctcttcc tggacgtcct gcgctccgcg gcgaaggacg ccgggcagcc gctccgggtg
     9661 gtggacgtcc tggggcagag ccgggaccat ccggtgctcc tcgcggcgcg cgagacgtcg
     9721 tatctcacga tggtgatcgc cgagcggatt cactgaggga gccatgaacg acaacgccga
     9781 atcgacgccg agccggaagg acctgtttct cggactggtc cattctttcc aggctgccgc
     9841 catgcagcag atggggaaga tcatgaaccc cttcaccgag aagatggaac gcgacttgaa
     9901 gcaggcgcgc ctctccatcg atatgctgga gatgctgcag gagcggacca gcgggaacct
     9961 gaccggggag gaggcgcgct tcctgaccca tgtgctgacg gagcttcggt tgaactacgt
    10021 ggccgagctg gacgaggaca agaaactggc cgcccagccc tcgaacgagc ccgcgccggg
    10081 ggtctccggc cctgccgagc cgggcgccta gccccggccc ggggctccgc ccccgggcac
    10141 tgcctccaac gttcttgctc ctgaccccgc ggcccggggt atccttgagg tccggggttg
    10201 tccgggccgc ttccggcgac gcgaacttgc gagggtgatt ccctcgatga acgataaaga
    10261 cgtccacgcc cccctgaatc tgccgggcgg cggggaaatg gcagcgctga tccgatcgat
    10321 cgactggtcc tccacgccgc tggggccggt ctcctcgtgg tcgcccacgc tccgcatgat
    10381 ggtgccgttc ctcgtagcga accgcttccc gctgctcctc tggtggggac cggactactg
    10441 ccagatctac aacgacgcct atcgtcccat cctcgggagc aagcatccgc gctcgatggg
    10501 gcagccgacg cgcgagtgct ggaccgagat ctgggatgtc ctcgggccgc tcatcgatac
    10561 gccctatcgc ggcggcccgc ccacctggat ggaggacctc gccctcgaga tcaaccgcca
    10621 cgggttcctg gaagagaccc acttcaccgt ggcgtacagc cccgtgcccg acgacacggc
    10681 gccggggggg atcggcggcg tgctcgccac cgtgcacgag atcagcgaaa aggtggtcgg
    10741 cgagcggcgg gtgcgggcgc tgcgcgatct ggccgccggc gggcccgagg ccaagaccgt
    10801 cgaggaggcg tgctcggccg cggggaagac gatcgagcga taccccaagg acattccctt
    10861 cgcgctcgtc tacctggtcg aaccggatgg cgcgcgcgcg acgctggcgt gcgcggtggg
    10921 gatcgaggag gatgggccgg ccagccctcg aaccatcgac ctcctgtcgg agcgtgcggc
    10981 gtggcccctg ggccttgcgc tccggaccga tgcgacgcag ctggtggagg acctcgcggc
    11041 tcgctttggc gccgcggtcc cgccgggccc gtgggccgac cctccgcgac aggcggccgt
    11101 gatgccggtc cgttcgacca tggcccggaa gtacgccgga ttcctggtcg ccgggatcag
    11161 cccgaggctc cgattcgacg agtcctaccg gagctttttc gagctggcga ccgggcagat
    11221 cgccacggcc gtcgccaacg cccgtgcgta cgacgaagag cggcgccgcg ccgaggcgct
    11281 cgcggcgatc gaccgcgcca agaccgcgtt cttctcgaat gtgagccatg aattccgaac
    11341 cccgctgacc ctcatgctgg gacctctcga ggatgcgctc gagcgcgggg cgctgccgga
    11401 cgaggagcgc gagcgcctgg agctgatcca ccgaaacgcg ctgcggctct ccaagctggt
    11461 caacgcgctg ctcgatttct cgcggatcga agcgggccgg gcccaggcga cctaccgtcc
    11521 caccgatctc ggcacgctca cggccgacct ggccagcgcg tttcggtcgg cgatcgagcg
    11581 cgcgggcctg gagctgatcg tccgctgccc gtccgtcggg gagccggcct gggtcgatcc
    11641 ggcgatgtgg gagaagatcg tcctgaacct tctctccaac gctttcaagt tcacgctcca
    11701 gggaacgatc gagatctccc tcgaaaccgt ggaccgggaa tggcggctga cggtccgcga
    11761 caccggcatc ggcatcgaag cgggcgaccg ggcccggatc ttcgaccgtt tccatcgcgt
    11821 cgagggcgcc cgagggcgct cgtacgaggg ctcggggatc ggactggcgc tcgtgctgga
    11881 gctggcccgc ctccacggcg ggtcggcgcg cgtcgagagc gccgtgggcc aggggagcgc
    11941 cttcgtcgtg cacattccgc gggggcacgc ccatctcccc cccgagcggg tcgtagccgg
    12001 cgaggcgtca cggcccgggt tggagcgggc gttcgtggag gaggcgctgc gatggctccc
    12061 cgattcggat gccgatcgcc tcgggtcgtc cgctcccgac gcgaacgccc cgatccgcgg
    12121 aggacgcgcg cgtgcgcctc ttcctcgggt cctcctcgcc gacgacaacg ccgatctgcg
    12181 cgactacgtc cgccgccttc tcgagcctca ttacgaggtc gagaccgttc cggacgggga
    12241 ggccgcgctg gagtcgatcc accgccgccc gcccgatctg gtcctctccg acgtcatgat
    12301 gccgcggcgc gacgggttcg ccctgctgcg cgctctccgg gaggacccgg acacggcgtc
    12361 gcttcccgtc atcctcctct cggcgcgcgc cggcgaggaa tcgcgggtcg agggactgga
    12421 gctgggcgcc gacgactacc tcgtcaagcc gttcgccgcc agggagcttc tggcgcgcgt
    12481 cgcctcgcga atcgtcgaca gcgagcggcg cgcctccgag tcgcgcgtgc gctacgtgct
    12541 ggagagcgtc tcggacggat tccaggtcct ggacgcggag gggcgctacc gttatgcgaa
    12601 cctcgccatg cggcgcctgt gggagggatt tgggcgctcg agcgaggtga tcggaaggca
    12661 cgtcttcgag gtctttccgg aggcgcggga gacgacgctg gggagggccc ttcagcgggc
    12721 gctcaccgag cgtgtgccga tcgagatcga gacgttctac gagccgttcc agcggtggtt
    12781 catggtgcgt tactacccca gtcccgatgg cggcctgtcc atcgtttcgc aggacatcac
    12841 cgcgcgcaag ctctcggaag accgggcgcg ggaggcggag cggcgcaagg acgagttcct
    12901 ggcgaccctc gcgcacgagc ttcggaatcc gctcgccccc atccggaacg ccatcgccat
    12961 tctccagtcg cgcggcaccg cggacctgga agcccgatgg gcgcgagggg tgatcgagcg
    13021 ccaggtggct tcgatggcgc gcctgctgga cgacctgctc gacatctcgc ggatcgcgaa
    13081 gaacctgctc cagctgagga aggaccgtca cacgctggcc gagatcgtgc agcggagtct
    13141 ggagacgagc cagccggcca tccaggcggc cgatcacagt ctcgccgtct cgctgcccga
    13201 ggaagatctg tggctcgagg ccgatcccct gcggctcgcc caggtgctct cgaatcttct
    13261 gaacaacgcg gccaagtaca ccccccgggg cgggcggatc gagctctcgg gccggaggga
    13321 gggatcggac gtcgtaatcg gggtccgtga caacggaatc gggatcgctc ccgacttcct
    13381 gcctcaggtg ttcgagatgt tcgtgcaggg cgctcccgcc gcggatgtat cggagggcgg
    13441 gctgggcatc ggcctctcgc tcgtgcaagg aatcgtgacg gcgcacggag gaagggtcca
    13501 cgcgagaagc ggaggtccgg gccaggggag cgagttcacc gttcgtctgc ccgccgcgcc
    13561 accgcgttcg gatgaagtgg ctgtccccgc ggcccagctc gaccgcgaga gcatcgcgcg
    13621 gaccaaggcg gtcacgtccg aggacgcgac cgtctagtcc cgctcgcgct cctcccgctt
    13681 cgccgcttcc cacagccggt ccagctccgg cagtcccgcc tcgtccacgg agcgcccctg
    13741 ctcgcggagc atcgcggcca cgcgatcgaa ccgtccccgg aacttctcgg tcgccagacg
    13801 cagatgcgcc tcgggatcga tccgcaggaa gcgcgagagg ttgaccaccg cgaagaggag
    13861 atcgccgatc tcctcggcga tccgctcggg tgcggccagg tccatctccc gctccacctc
    13921 cgccagctct tcgcggatct tctccaccac gccctgcgcg tgctcccagt cgaagcccac
    13981 ggacgcggcc ttctcctgga tgcggtaggc ctggagcagg gcgggcagcg cgtcgggccg
    14041 cttgcccagg atggaggcgt ccgccgactc cttccgcttc cgctcctccc accggcgcag
    14101 cacgccctgg gcgtccagct cgggttccgc ggcctcgtcg ccgaacacgt ggggatggcg
    14161 cccgatcatc ttggcgatgt tccggcgcag caccgcggcc ggggtcgcgc cgccttcgcg
    14221 ctcgagcgcc accgcgagga agcaggccag gtagaagaga tcgcccagct cctcggaggc
    14281 gccgtcggcg ttggcctcgg cgaccgcttc gccgatctcg tgcgcttcct cgacgacgta
    14341 gggcgtgagc gtgcgcggcg tctgctcgcg gtcccacggg cagccctcgt ccgaagcgag
    14401 gtggcgcacc agatcgagca gctcggcggc cgcggcggcg aagtccgagc gatcccgggc
    14461 ggggggcgag agcggcgggc gcggcgcgtc catcgggcca gtctagccga gagggccgcc
    14521 gcggccccag cgaaagccgc cgttcttgcg cgcgccccgg acgtgtgata ctcgtggcta
    14581 gccgggaaac cggcgcgcct atggaatttc cgacctgggt cgaagtcgac ctcaaccgct
    14641 tccgccggaa tctcgcggcc atcgccacgg ccatcgggcc ccggcggcgg atcctcctcg
    14701 tcgtcaaagc cgacgcctac ggccacggcg ccgtcgagat cgcgcgctgc gcggtccaga
    14761 acggcgtctc gatgctgggg gtggccaccc tgcacgaggg gatcgagctg cgctcggcgg
    14821 gcatcgacct cccggtcgtc atcctgagcc cttgcctcct ctccgaagtg gacgagatca
    14881 tcgagcaccg gctcacgccc tgcgtggcca cgctcggctt cgcgcagcgg ctctccgagc
    14941 gatgcgccgc gcaccaggtg ctctgccgcg tccacgtgga ggtggacacc ggaatgggac
    15001 gcgcgggggt cgccgagtcg gaggccgtgg acttcgtcga gcggctgacc agcctcccga
    15061 acctccgcct cgaggggatc ttcacccatt tccccgacgc cgactccggc cgcaccgacg
    15121 tgagcgagga gcagcttcgc cgtttcggtg ccgtgctgca tgcgctggag ctccggaaga
    15181 tcgaggtgcc ggtccgccac gccgcgaaca gcgcgggcat cctgagcgtg cacgactcct
    15241 acctcgacat ggtgcgcccc ggcatcctgg cctatggctt ctatccgagc cgcgccgtgc
    15301 cgcacagcgt ggaggtggag ggggtgatgg cgttcaagac gcgcatcgtc cagctccgcg
    15361 atcttccgcc cgggcgtttc gtctcctatg gccgcacctt ccaggccccg gggtggatgc
    15421 ggatcgcggt gctggcggtg ggctacggcc acggctatcc gtgggcgctc tccaaccgcg
    15481 gcgaggtgct gattcgagga cggcgcgcgc cggtcgtggg gcgcgtcacg atggacctca
    15541 cgatggtgga cgtctcggcc attcccgagg cgatgccgga ggacgaggtg gtgctctggg
    15601 gggagcaggg gggcgcgcgg ctcgatctgg gcgaggtggc cgagcgggcg cagaccatcc
    15661 cctacgacct cctctgcagc atggggaagc gcgtcgtgcg cctctatcac aacgacggcg
    15721 agccgatgaa ggccctgacg ctgatcggcg tgcgcgagga gaccgaggtc atcgaggggg
    15781 gcggcgccgc gggaccgcgc cgccgacgcc gacgcgtgcg ctatcgcagc acgaggagcg
    15841 gatcgtgagc gccggggtag ggggaggggc atgagcgacg gcgtgaagct gctcgaggcc
    15901 gcgcgggtcc tccttgcgaa agcgcatgcg ccctattcgc actaccgggt cgcggccgcc
    15961 ctcgaggacg aacacggcgt cgtccacccc ggcgtcaacg tggagagcgc ttcgctcggg
    16021 ctcacgatct gcgccgaacg gaacgcgctc ttcggcgcgc tggcccgcgg cgcgcggcgg
    16081 tttcgccgca tcgcgatcgc caccgatcct cccggcccct gcgtcccctg cggcgcctgc
    16141 cgccaggtcc tcgtcgagca cgctcccgac ctcgaggtga tcgtcgcctc gcccgagggc
    16201 gcgcccgaga tgattccgct tcgcgatctc ctgccgcgcc ccttcctcac gttctgatgc
    16261 gcgacgtcat cgccgccaag cgcgacggcc gggcgctgtc cgaggcccag atccgcgcct
    16321 ggatccgcgg ggtgaccgac ggttccattc ccgactacca gagcgccgcg ctgctcatgg
    16381 cgatcgtctg gcgcggcatg agtcgcgacg agacggccgt cctgaccgac gccttgatgc
    16441 gctcggggcg cgtgctcgac tggagcgggc tcgggcgccc cacggtggac aagcactcga
    16501 cgggtggcgt aggggacaag gtctcgatcg cgctcgcccc ctgggtggcg gcgtgcggcg
    16561 cggtcgtgcc gatgatcgcc gggcgcggcc tgggccacac gggcggcacc ctcgacaagc
    16621 tcgaggcgat tcccggcttc cgaacccgcc tatccgccgc cgagttcgag ggccaggtgc
    16681 ggaagatcgg agtcgcgatc gcggggcagg gcgaagacct cgcccccgcc gacggcgcgc
    16741 tctacgcgct gcgcgacgtg accgggacgg tggagtcggt gccgctgatc gtggcgagca
    16801 tcctgagcaa gaaagccgcg tcgggtgcgg cgggcgtcgt gttcgacgtg aagtgcggcg
    16861 ccggggcctt catgccgggc cgcgaggagg cggcggcgct cgcgcgggag ctggtcgcgg
    16921 tgctctccgg catggggcgg aaggcgcacg cgctggttac cgcgatggac gagccgctgg
    16981 gtgctgcggt cgggaacgcg aacgagacgg ccgaggcgtt cgcggtgctg caccgggccg
    17041 cgcccgcaga tctcatggac gtgacgcagg cgctcgccgt ccggatgctc gtcctgagcg
    17101 gggtcgcgcg cgagcggcac gaggccgagg cgcgactgga ccgtgcgctg gagacgggcg
    17161 aggcgctggg ccgcgccgag cgcatggtgc aggcgcaggg cggcgatccg cgcgtggtcg
    17221 gggatccggg ccgcttgccg agggccccgg tggagatggc ggtccccgcg ttgcgggccg
    17281 gctacgtcgg cgcggtggac gcgcggcgga tcggcgagct cgtgatcgcg atgggcggcg
    17341 gccggcgccg caaggaggac gtggtggatc ccgcggtggg cgtgcgcctg ctgcgcaagg
    17401 gcggcgagcg cgtcgatgcc ggcgaggtcc tggcgctgat ccaggcccgt cgcgacgccc
    17461 cggagtggac ggaagcggcg gcgcgcgcct tcaccatcgc cgacgagccg ccggacaagt
    17521 cgccgctggt gctggaggag atcgcgccgt gaagcgcgct tcgcgggggc gttcggcgcc
    17581 gtcccgaggg gacctccgcg tggacccggc gacgcccgat cgctgggacg atcttgcggc
    17641 tctgttcggc ccccgcggcg cgtgcgccgg ctgctggtgc atgtggtggc ggcgctcggc
    17701 ggccgagtgg aagcgcggta agggagacgg aaaccgccgc gcgctccggc gcctggtgga
    17761 ttccggcgag ccgccgggct tgatcgcgta cgacggggaa acgccgatcg gatggatcgc
    17821 gctggcgccg cgggaggcct acgcgcgcct ggcctcggcg cgctcgctca agccggtgga
    17881 cgacacgccg gtctggtcgg tcacgtgctt cttcgtgacg aaggactatc gccgtcgcgg
    17941 cgtcagcgcg catctcctgc gcgcggccgc ggagcacgcg cggaagcgcg gggcgaagat
    18001 gatcgaaggc tatcccgtcg tgagccgcgc ggggaggatg ccggacgcgt tcgcgtggac
    18061 gggccttccg agcgcgttcg agcgcgcggg atttcgcgag gtcgcgcgac cgtcggcttc
    18121 gcggcggatc atgaggaagg cactgaagaa gagctagcta cagcctcaga tgctcctcgg
    18181 gctgcgcctc gctgtcgtgc tgctccagat cacgcggctc cgattccggc ggcggggtgg
    18241 cgccggtgtg gatgtcgcag tagccggtcg gctccgtccc ctctttgaag agctccatct
    18301 ccgtctcggg gcaggcggga ttggcgagca ggcccgtctg gctgcagatc aggcgcgcca
    18361 ccacgccgtt gggaacgggg aaatcctgtg cgggcttgtt cttggttccc gccgagacca
    18421 cgtcgatcca gatcgggagc gccgccgcgg cgcccgtcat gttcggcccg atcttctgct
    18481 tccggtcgaa gcccacccag acgcccgtga ccagcgaggg cgtgtagccc acgaaccagg
    18541 cgtcggtgta gtcgtcggtc gtgccggtct tgcccgccgc ggggacgttc aggcccatcg
    18601 cgtgcgccgc ggccgcggtt cccgactgca gcaccgtctc catcatgttc gtcatcgtga
    18661 gcgcggtctc ggggctcagc acttcttcgg ccgtcgtgcg cgtctgctcc agaactttcc
    18721 cgttcttgtc ctcgacgcgg agcacgaaca cgggcgtggt gcgcacgccc tggttggcga
    18781 agacgccata ggacgacgtc agctcgagca gattcacttc cgacgtgccg agcgcgaggg
    18841 agagatcgct gcccaggcgg ctccggatcc ccagccgccg cgcgtacgag atcacctgcg
    18901 gcgtcccgat cttctggagc agcttggccg ccgggacgtt gagcgacttg gccagcgccg
    18961 tgcggagcgt caccggcccg cggaacttgt ggtcatagtt ctggggcgac cagtcctcgc
    19021 cgttccccgc cttgaaggag accggggtgt ccaggatcat ctcgttcggc gtgtagccgt
    19081 tgtcgatggc ggccgtgtag atgaacggct tgaacgccga gcccggctgg cgcgccgcct
    19141 gggtcgcgcg gttgaagttc gattcgttga agtcgcggcc gccgatcagc gcgcggatct
    19201 gcccgttcgt gggatcgatc gccacgacgg cgccctgcag gtagtcggtc tgcgtccgct
    19261 cgctcggctg cgccgtcttc gccttggcca gaatcgccgc gcggctgcgc ttcccgccga
    19321 tccgctgctc cagctcggtc agacgccgct ccagcgattc ctcggcggcg tgctggagat
    19381 cggcgtccag cgtcgtgtag acgcgtagcc cgccctcgaa gagctggttc gagccgtagc
    19441 gctcttccag gtagagccgc accatctcca tgtaataagg agctgacttc gtgtcgatgc
    19501 gggtcttgct cacgccgagc ggcgtctccg aggcggcctc gtactgcttc tggctcagct
    19561 tcttgttctg gaccattgcc gcgagaacca ggttgcgccg cttgagcgcg cggtccggat
    19621 cgcggcgcgg cgcgtagtcg cgcgggttgc gcggcagccc cgccagcagc gcgcactcgg
    19681 gaagggtcag ctcctgcacc gacttcccga aatagatgcg cgcggcggcg tccacgccgt
    19741 aggcgccctc gccgaaatag atctggttca ggtacatctg gagaatctcg tccttggtgt
    19801 acgtctgctc gatgcggacg gcgaggatcg cttccttgat cttccgcgag accgtcttct
    19861 cgtgggtgag gaagagattc cgcgcgagct gctgcgtgat cgtgctggcg ccctgctcgg
    19921 ggcgccgcgc gatcacgtcc tgcaccagcg ctccgaagag acggacgggg tcgatgcccc
    19981 agtgcgagta gaagcggcgg tcctcgatgg agaggatcgc gtccaccatc ggtttcggga
    20041 tctgcttcag cgggacgacg ctccggttct ccttgtagaa ctcgtgcacc agcttcccgt
    20101 tcacgtcgta gaccagggtc ttcacgggag gcgcgatggt ctgcaggctg gagggcgagg
    20161 gaagatcctg gcgcgcccag ttggcgacgc cgattccgac gccgacggtg gcgcacagga
    20221 cgacgatgag gagtgccgcg gtgaactggg cgatccactt ccagcgatgg ctcacggaag
    20281 gtccttggct cttggtccga gggcgggcac gaaacgtacg ctaggttccg tgcgccgggg
    20341 ggtcaagcgc gactttcgcg ctccgggcaa agttgctaga gttcgccgtc catgaacgac
    20401 aaaccgattc tgagcgccga ggcggcgcgc cagctcgcgg tcatccgctc gggcgtcgag
    20461 gagctgattc ccgaggccga gctggtgaag aagctcgagc gctccctcgc gaccaagacc
    20521 ccgctccgcg tgaagcaggg attcgatccc acggcgcccg acatccacct ggggcatacc
    20581 atcgggctcc gaaagctccg gcagttccag gagctgggcc accaggtggt cctcatcgtc
    20641 ggctcctaca ccgcgctcgt cggcgatccc agcggccgca acaagacgcg cccgcgcctc
    20701 accgaggccg aggtggaagc gaacgcgcag acctacctcg ctcagttctt caaggtcgtc
    20761 gacaagtcga agaccgaggt ccgctggaac ggggagtggt tctccgcgat gcgcttcacc
    20821 gacgtgcttc atctcacctc gcagttcacc gtggcccgtc ttctcgagcg cgacgatttc
    20881 gaccagcgct tcaaagccgg cgagccgatc ggagtgcacg agctcttcta ccctctgatg
    20941 caggcctacg attccgtcgt gatccgggcc gacgtggaaa tcggagcgac cgagcagaag
    21001 ttcaatctcc tgacgggccg cgatatccag atggcctacg gcgtcgagcc ccaggtgatt
    21061 ctcacgctcc ccgttctcga gggcttggac ggcgtgcggc gcatgagcaa gagcctcggt
    21121 aactacatcg gcgtttccga gtcgccgaag gagattttcg gcaaggcgat gagtctcccc
    21181 gactccatga tcgagcggta cttccgcctg gtcaccgacg tctccgacca agagtgcgcg
    21241 gaagtcgcga aatcccttcg ggatccgtcg gtcaatccga tggtgtggaa aaaagctctg
    21301 gcccaccagc tggtgcgcca gtaccacggc catgaggagg ccgatcgggc ccgggacgat
    21361 ttcgagaagc agttttcgcg ccgcgagata ccgtccgaaa tgcccgtcgt tacggtcgaa
    21421 tcgggccgtt ttcgcgcccg ggacctggtc atgcgggcct ttcccaatca gtacaccggc
    21481 accgccgcgg ggaatctatt ccggcagggc gccgtccata ttggagggga gcgggtgacc
    21541 gacatcgccc gcgagttcga cctccgtaag ggctcggaaa cagaggagtt ggtcatcaag
    21601 gtgggccgga agttcgccaa ggtcgtggcg gcctgatcgg acctcggggc ctgtttgggc
    21661 aagttattgt gcaaatgcga cttgccggcc cagggatttg tgctggggtg gggttgacag
    21721 ggagggataa cacagctatc gtctcttctt gcggccggca
//
LOCUS       DASSLZ010000155         8960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1060181, whole genome shotgun sequence.
ACCESSION   DASSLZ010000155 DASSLZ010000000
VERSION     DASSLZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8960
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060181"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="VFM00_06070"
     CDS             <1..1583
                     /locus_tag="VFM00_06070"
                     /inference="COORDINATES: protein motif:HMM:NF045502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7497846.1"
                     /translation="VIPGKLKDIWMNVPVEEDFAAASPYVIGRDSGGYHAHDTRTGYV
                     YSIRIPEEPAWYARRTARGTEMAKVGVLQGTYLGIYIANSCLFWYSEPPQNCRFCTTG
                     SNVGVNEVAQKDVADVVEVARAAKEESGVTFVHLNSGHQAGRDLALAEPYVRALKEEV
                     GVLVGVQLMPSKDLRQYDRLVEMGVDHFSFCYEFHSPEAFARYLPGKERWFGQKAFFE
                     AMEHCARLLGKGAVSGEIIAGVEPVADTMRAIDYITGIGAFPTVCIFRPVIGAEMERH
                     PAPRYDDMRAVFEHVYEACRKNRIPIGAAPNIEVSLVVQPDDTRYLARPGLSTWAYES
                     FLGAARAGAKPLFARKMRRELRWPQAAAAPQAAQLPRPASDVVMGAGAQPANGAAQAD
                     PVETAATTPGATVPSPEPIETVFARLRHAAAETSRPFRLEERSGVLDVAPSQRERCRM
                     KVFRPREGRDRLCAFFYKESNLSWSRDRYSYGAVEFLPAQVTETDMRTWLDWLAGGFD
                     PDHRPPRLKKAFLYSIPD"
     gene            1654..2100
                     /locus_tag="VFM00_06075"
     CDS             1654..2100
                     /locus_tag="VFM00_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7497847.1"
                     /translation="MKTARKRLLIVDDDHDALASLKLLLETSYDVCLADSGVQALTEM
                     NRGFYPDAVLLDLMMPTMDGVEFAREMTRVGVRTPILVISGDPDAERKAREIGNAEML
                     RKPFTYEQLKAKLERVLNGRQTPRSGGGLGFIRGLFVLVPSRLGGA"
     gene            2090..2536
                     /gene="mutT"
                     /locus_tag="VFM00_06080"
     CDS             2090..2536
                     /gene="mutT"
                     /locus_tag="VFM00_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HET7497848.1"
                     /translation="MARNPAGRTSPVRLYEVAAGILVAGDRVLIARRQERDHQGGRWE
                     FPGGKRHTGETVEECLRREMLEEIGLQVQVGPLWRALTHVYPDRRVSIYFHLCTSYQG
                     EPVAIECDEIRWVLPVELTTLPFVEGDLVVLPDLARELAERFPTPA"
     gene            complement(2658..3320)
                     /locus_tag="VFM00_06085"
     CDS             complement(2658..3320)
                     /locus_tag="VFM00_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET7497849.1"
                     /translation="MFARRRSLAATLFAAALFVSTAAQAAPTTWTIDPNHSNVTFTIR
                     HFFSKVTGSFTKFSGSVVYDPANPAASSAKAEIDASSINTSNDRRDGHLRSPDFFDVA
                     KYPTLTFESTKVTADGSKLKIEGNLTMHGVTKPVTLEGEFLGAGPAMGGQRAGFEAST
                     KVDRKDFGIVWNKVVDQGTMLGDDVEIRLAIEGATEPAGGAKKAEAGGKNDEKKAEAN
                     AK"
     gene            3551..4531
                     /gene="trxB"
                     /locus_tag="VFM00_06090"
     CDS             3551..4531
                     /gene="trxB"
                     /locus_tag="VFM00_06090"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018542675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET7497850.1"
                     /translation="MTGTRNLIILGSGAAGLTAAIYAARANLAPLLFEGIQPGGQLTM
                     TTDVENFPGFRDGIMGPALMTEMRAQAERFGTDFVQATVEAVDLKGHPFRVTADGVTY
                     TARALIVATGASAKLLGIPSEALLMGHGVSACATCDAFFFRGKRVIVVGGGDTAMEEA
                     TFLTKFATHVTIVHRRDELRASKIMQERAMSNPKISFIWDSEIGEVLGTEVGHVTGVR
                     LRNLKTGKLTEVATDGVFIGIGHQPNTEIVQGQLELHPNGYIKTHGGTRTSVPGVFAA
                     GDVQDHVYRQAITAAGTGCMAALDAERYLEALSHGHPEAGAAAPAKVPSA"
     gene            4575..6272
                     /locus_tag="VFM00_06095"
     CDS             4575..6272
                     /locus_tag="VFM00_06095"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HET7497851.1"
                     /translation="MNDVQRDVLEVDVLFVGGGPAGLAGALRLTQLVAERNAAAASGS
                     GEPPLGEVSIAVLEKASEIGAHGISGCILDPRALRELVPDYREKNAPLESDVTSDDLY
                     YFTKSGAIKFPILPPPLQNHGTHIVSLGDFEKWLGGLVEAAGAYVLPNMAAVEGLFEG
                     EQMVGVRTGDKGIDKNGARKANFEPGSDIRAKVTVLCEGARGSLTKRLTPRLLLDEGR
                     APQIYATGIKEVWQMPAGRVPKGRVIHTMGAPLDSRTFGGGFVYGMDHDRWAVGFVVG
                     LDYWNPLTDPHGLFQEYKRHPMIAGLLEGGTVLSYGAKAISEGGWYSVPRLSWPGGLL
                     AGESAALLNSQRLKGVHLGMKSGMLAAETAFDALIAGRFDAEAMRRYDERIEGSWVRQ
                     ELWGVRNFHQGFERGLWMGMADAGLQLVTGGLGFGNRPRWKPGPMHMEKLKEAGVAPI
                     SVDEVQRRYDNAKTFTKVNDVYYSGTKHDEDQPAHLLVQDRTICSTRCKEEYGNPCQH
                     FCPANVYEMEPAPEGGIRLKLNPSNCVHCKTCDIADPYGIITWVPPEGGGGPNYQHL"
     gene            complement(6580..6918)
                     /locus_tag="VFM00_06100"
     CDS             complement(6580..6918)
                     /locus_tag="VFM00_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7497852.1"
                     /translation="MRDHFQEFEISPELVREMIEDGDDAVILLDVRDDWEWEVAHLEG
                     AIHIPLDELGLRIQELDPHQEIVTYCHVGERSVDACLLLWENGFRKVRSMTGGIEAWS
                     ELVDPTVPRY"
     gene            complement(7018..7764)
                     /locus_tag="VFM00_06105"
     CDS             complement(7018..7764)
                     /locus_tag="VFM00_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HET7497853.1"
                     /translation="MNVSYPSWWRPLGRALWGAAFLLLAVSAWMILSKAPIEVQMGPV
                     QKIVYVHVPSAIATLLAFGFTFACSIAYLTTKSWVWDAMAASACELGLVFATVVMVTG
                     PLWARSAWNTWWTWEPRLTTFLILWILYAGYHVVRASLHSGAKRTVSSILGIVLFVNL
                     PIVWMSIQWWRGSLHPQKVTMTGDMRATLLESMLAWIVFLAAAFVTRLRLEWAREAAM
                     DRESEMAPGLARHGRDPRDPAGPAGGRVSA"
     gene            complement(7790..8461)
                     /locus_tag="VFM00_06110"
     CDS             complement(7790..8461)
                     /locus_tag="VFM00_06110"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET7497854.1"
                     /translation="MSFLAVALKDLRLYARDARFVATLLLFSASLVLILSVAFGPIFK
                     DPAREASATLWAALFFAGVLGLTRSLDLEAESGGGDAMRLTGADPYAIYLGKTIANIL
                     ILGGVFVLVLPIVSVFFDVGNLRVLPGVLGVAALGVVGYAAWGTLFAALARGLKAREL
                     LLSVLLFPSLVPLWIGAVKITHSLWAEGTAGPVQDWLKVMAVSDVLGLALAAFLYELI
                     QEASE"
     gene            complement(8526..>8960)
                     /locus_tag="VFM00_06115"
     CDS             complement(8526..>8960)
                     /locus_tag="VFM00_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7497855.1"
                     /translation="REARRAMGFASHRPLLYLGLTPLENLSLFGKLTGVRHPDQRALE
                     LLERFGMGAFAKTPVERFSRGMLQRVSLARAFLGEPRLLLLDEPYTALDDDGTAELNG
                     LIGEARDRGAATMIISHDRERLGALRTRLCLMQEGRVVEDGA"
BASE COUNT         1509 a         3054 c         2989 g         1408 t
ORIGIN      
        1 tcgtgatccc cggaaagctc aaggacatct ggatgaacgt cccggtcgag gaggacttcg
       61 ccgccgcctc gccctacgtg atcgggcgcg actcgggcgg ctatcacgcc cacgacacgc
      121 gtaccggcta cgtctattcc atccgcattc ccgaagagcc ggcgtggtac gcgcgacgca
      181 ccgcgcgcgg caccgagatg gcgaaagtgg gcgtgcttca gggcacctac ctcggcatct
      241 acatcgccaa ctcctgcctc ttctggtatt ccgagccgcc gcagaactgc cgcttctgca
      301 ccaccggctc caacgtgggc gtgaacgagg tggcgcagaa ggacgtggcc gacgtggtcg
      361 aggtcgcgcg cgcggcgaag gaggaatcgg gcgtcacgtt cgtgcatttg aacagcgggc
      421 accaggcggg ccgcgatctc gcgctggccg agccgtacgt gcgcgcgctg aaggaagagg
      481 tcggcgtgct cgtgggcgtg cagctcatgc cctcgaagga tctccgccag tacgaccggc
      541 tcgtggagat gggcgtcgac cacttctcct tctgctacga gttccactcg ccggaggcgt
      601 tcgcgcgcta tctgccgggg aaggagcgct ggttcgggca gaaggccttc ttcgaagcga
      661 tggagcactg cgccaggctc ctcggcaagg gcgcggtctc gggcgagatc atcgccgggg
      721 tcgagccggt cgccgacacg atgcgggcga tcgattacat caccggcatc ggcgccttcc
      781 ccacggtctg catcttccgc ccggtgatcg gagcggaaat ggagcgccac ccggcgcccc
      841 gttacgacga catgcgcgcg gtgttcgagc acgtgtacga ggcgtgccgg aagaatcgga
      901 ttccgatcgg cgccgcgccc aacatcgaag tgagcctggt cgtgcagccc gacgacacgc
      961 gctacctggc gcggccgggc ctgtcgacct gggcctatga atccttcctc ggggcggcgc
     1021 gcgctggagc caagccgctc ttcgcgcgaa agatgagacg tgagctgcgc tggccgcagg
     1081 cggcggcggc accccaggcc gctcagctgc cgcggccggc cagcgacgtc gtgatgggcg
     1141 ccggcgcgca acccgcgaac ggcgccgcgc aggcggatcc cgtggaaacc gcggcgacga
     1201 cgcccggcgc gaccgtgccg tcgcccgagc cgatcgagac ggtcttcgcg cggcttcgcc
     1261 atgccgcggc ggaaacgtcc cggccgtttc gactcgaaga gcggagcggc gtcctggacg
     1321 tggccccttc ccagcgcgag cgatgccgca tgaaggtgtt ccgtccacgc gagggaagag
     1381 accggctctg cgccttcttt tacaaagaga gcaacctcag ctggagccgc gaccgctact
     1441 cctacggcgc cgtggaattt ctgcccgcgc aggtaaccga aacggacatg cggacctggc
     1501 tcgattggct ggccggcggc ttcgaccccg accatcgtcc tccccggctc aagaaggcgt
     1561 tcctctactc catccccgac tgagcggccg ccattcactc gatcgggctc tcgcttcatt
     1621 gacccgactg agtcgaaccg tcacactgta cctatgaaaa cagcccgaaa gaggctcctg
     1681 atcgtcgacg acgatcacga tgcccttgcc agcctcaagc tgttgttgga aacgagctat
     1741 gacgtctgcc tcgccgacag cggagtgcag gcgctgaccg aaatgaaccg gggcttctat
     1801 cccgatgccg tgctgctgga tctcatgatg cccacgatgg atggcgtcga gttcgcccgg
     1861 gagatgacgc gcgtgggtgt gaggacgccc attctcgtga tctccggcga ccccgatgcc
     1921 gagcggaaag cccgcgagat cggaaacgcc gagatgctgc gcaagccgtt cacctacgag
     1981 cagctcaagg cgaagctcga gcgtgtgctg aatgggcggc agaccccacg gtcgggaggc
     2041 ggcctcggat tcattcgagg gctattcgtc ctggtgcctt cacggctcgg tggcgcgtaa
     2101 ccccgcgggg cgtacctcgc cggtgcggct ctacgaggtc gcggcgggca tcctggtcgc
     2161 cggcgaccgc gtgctgatcg cgcgccgcca ggagcgcgac caccagggcg gacgctggga
     2221 gttccccggc ggcaagcgcc acactggcga gacggtcgag gagtgcctgc gccgggagat
     2281 gctcgaggag atcgggctcc aagtccaggt gggccccctg tggcgcgccc tcacccacgt
     2341 ctaccccgac cgccgcgtca gcatctactt ccacctctgc acgtcgtacc agggcgagcc
     2401 tgtcgccatc gagtgcgacg agatccgctg ggtgctcccg gtggagctga cgacgcttcc
     2461 cttcgtcgag ggcgatctgg tcgtcctgcc cgatctcgcg cgcgagctgg cggagcgctt
     2521 cccgacgccg gcctgaaaag aaacagcgag ctgcggccac gcgcccgagg cgcgtcgcgt
     2581 cagctcgctg gattgcccta cttcgattcg ttgaacgcgc gatcgattcg ccgaaatcgg
     2641 ccgttcgcgc ggcccggcta cttcgcgttc gcctccgctt tcttctcgtc gttcttcccg
     2701 ccggcttccg ccttcttcgc gccgcccgcg ggctccgtcg cgccctcgat ggcgagccgg
     2761 atctcgacgt cgtcgccgag catggtgccc tggtccacga ccttgttcca gacgatcccg
     2821 aaatccttcc ggtccacctt ggtcgaggcc tcgaaccctg cccgctggcc gcccatcgcc
     2881 ggacccgctc cgaggaactc gccctcgagc gtgaccggct tcgtgacgcc gtgcatcgtc
     2941 aggttcccct cgatcttcag cttcgacccg tccgccgtca ccttggtgct ctcgaacgtg
     3001 agcgtcggat acttggccac gtcgaagaag tcgggcgagc gcagatgtcc gtcacgccgg
     3061 tcgttcgagg tgttgatgct cgaggcgtcg atctctgcct tggccgagct ggccgccggg
     3121 ttcgccggat cgtagaccac cgagcccgag aacttggtga acgagccggt caccttgctg
     3181 aagaagtggc ggatggtgaa ggtcacgttc gagtggttcg ggtcgatcgt ccaggtggtc
     3241 ggagccgcct gggcggccgt cgaaacgaac aacgccgcgg cgaacagcgt ggcggcgagg
     3301 gaacgtctgc gggcgaacat cgtggtcttc ctccgtgggg ttggaaggcg gaggggcgcg
     3361 gggagacgcc cccctgccgt cgcgcgacct cgagggggct accaaagtcc cggggccccg
     3421 gagttgatcg gtcgtcgcgg tttgcctact atctcgtgga cccacacttg gatgcggccg
     3481 tggcgagccc gattccggcg gcgaaccgcg tgcagacgca tctaaaccct tacgaggaca
     3541 gtgtgttccc atgaccggta cccgcaatct catcattctg ggctcgggcg cggctggcct
     3601 gaccgcggcg atctacgccg cgcgggccaa tctggcccct ttgctgttcg aggggatcca
     3661 gcctggtggc cagctcacga tgacgacgga cgtcgagaat tttcccggct tccgggacgg
     3721 gatcatgggt ccggcgctga tgaccgagat gcgggcccag gccgagcgct tcgggaccga
     3781 cttcgtccag gccaccgtcg aggcggtgga tctgaaaggc caccccttcc gcgtcacggc
     3841 cgacggcgtc acctatacgg cccgcgcgct gatcgtcgcc accggcgctt cggccaagct
     3901 cctcgggatt ccgtccgagg cgctgctcat gggccacggc gtctcggcct gcgccacgtg
     3961 cgacgccttc ttcttccggg ggaagcgcgt gatcgtcgtg ggcggcggcg acaccgccat
     4021 ggaagaggcg acgttcctga ccaagttcgc cacgcacgtg accatcgtgc accgccgcga
     4081 cgagctccgc gcctcgaaga tcatgcagga gcgcgccatg tccaatccca agatctcctt
     4141 catctgggac tcggagatcg gcgaagtgct cgggacggag gtggggcacg tcacgggcgt
     4201 gcggcttcgc aatctgaaga cgggcaagct caccgaggtg gcgaccgacg gcgtcttcat
     4261 cggaatcggc caccagccca acaccgagat cgtgcagggg cagctcgagc tgcaccccaa
     4321 cggctacatc aagacgcacg gcggcaccag aaccagcgtg cccggcgtct tcgccgcggg
     4381 cgacgtgcag gaccacgtct atcgccaggc gatcaccgcg gccggcacgg gatgcatggc
     4441 cgcgctcgac gccgagcggt acctggaagc gctgagccac ggccatcccg aagcgggggc
     4501 cgcggcgccg gcgaaggtgc cgtcggcgtg acggatttcg cgagcccggc ggccctgctc
     4561 gtgaatggtg ccgcgtgaac gacgtccagc gcgatgtcct cgaagtcgac gtgctcttcg
     4621 tcggaggcgg accggcgggt ctggccggcg cgctccgcct gacgcagctg gtcgcggagc
     4681 gcaatgccgc ggcggcgtcg gggtcgggcg agccgccgct gggcgaggtc tcgatcgccg
     4741 tgctcgagaa ggcctccgag atcggcgccc acggcatctc cggctgcatt ctcgacccac
     4801 gcgccctgcg cgagctggtc cctgactatc gcgagaagaa cgccccgctg gagtcggacg
     4861 tcaccagcga cgacctctat tacttcacga aatcgggcgc gatcaaattc cccatcctgc
     4921 cgccacccct ccagaaccac ggcacgcaca tcgtctcgct cggcgatttc gagaagtggc
     4981 tcggcggcct ggtcgaggcg gccggcgcct acgtgctccc caacatggcc gccgtcgagg
     5041 gattgttcga gggcgagcag atggtgggcg tgcgcacggg cgacaagggg attgacaaga
     5101 acggcgctcg gaaggcgaat ttcgagcccg gctccgacat ccgcgccaag gtgaccgtcc
     5161 tgtgcgaggg cgcgcgcggc tcgttgacga agcgcctgac gccgcggctc ttgctggacg
     5221 agggccgcgc gccgcagatc tacgccaccg gcatcaagga ggtctggcag atgccggcgg
     5281 ggcgcgtgcc gaagggccgc gtcatccata cgatgggcgc gccgctcgat tcgcgcacgt
     5341 tcgggggcgg cttcgtctac ggcatggacc acgaccgctg ggccgtcggc ttcgtcgtgg
     5401 ggctggacta ctggaacccg ctgaccgatc cccatgggct gttccaggag tacaagcgcc
     5461 acccgatgat cgccgggctg ctcgaggggg gaaccgttct ctcctatggg gcgaaagcga
     5521 tctccgaggg gggctggtac tcggtgccgc ggctgtcgtg gccgggcggg ctgctcgccg
     5581 gggagagcgc ggcgctcctg aattcgcagc gcttgaaggg cgtgcacctg gggatgaagt
     5641 cgggaatgct cgccgcggag accgcgttcg atgctttgat tgccggccga ttcgatgcgg
     5701 aggcgatgcg ccgttacgac gagcgcatcg agggctcgtg ggtgcggcag gagctctggg
     5761 gcgtgcggaa cttccaccag ggcttcgagc gcgggctgtg gatgggcatg gccgacgccg
     5821 gactccagct cgtgacgggc ggactcggtt tcggcaatcg cccgcggtgg aagcccggac
     5881 cgatgcacat ggagaaattg aaggaggcgg gggtcgcgcc catttccgtg gacgaagtgc
     5941 agcgccgcta cgacaacgcc aagacgttca ccaaggtgaa cgacgtctac tactcgggca
     6001 ccaagcacga cgaggaccag cccgcgcatc tgttggtgca ggaccgcacc atctgctcga
     6061 cccgctgcaa ggaggagtac ggcaatccct gccagcactt ctgccccgcc aacgtctacg
     6121 agatggagcc cgcccccgag ggagggatcc gcctgaagct gaatccctcg aactgcgtcc
     6181 actgcaagac ctgcgacatc gccgacccct acggcatcat cacatgggtc ccgccagaag
     6241 gcggcggcgg cccgaattat cagcatttgt agtcaagctg gtccgagggc cgcgcgccaa
     6301 gccagccacg acatactctg gagtatgttg tcgtgccaat ccggctcact gggcaatatt
     6361 gaagagcagc ttgcggcgag gtcaacttgc gccggcgcaa gttaatattt atattaatag
     6421 cttcccgctt ggagttgccc tcgattccgt ttcgatccga atgccgccga gttacccgcg
     6481 ccagagcatg tgaaatcggc gacatttgtc gcttcgggta gacaaaccaa ctagcacatc
     6541 gggacgtccc gctttggccg agcggcttcg tttactgcgc tagtaccgcg gcacggtggg
     6601 gtccaccagc tcgctccagg cttcgatccc tccggtcatg ctcctgacct tccggaatcc
     6661 gttctcccag agtaacaggc aggcgtcgac gctgcgttcg ccgacgtggc agtaggtgac
     6721 gatctcctga tgggggtcga gctcctggat gcggagtccg agctcgtcca gagggatgtg
     6781 gatcgctccc tccaggtggg ccacttccca ctcccagtcg tcccgcacgt cgaggaggat
     6841 gacggcatcg tcgccgtcct cgatcatctc gcgcaccagc tcgggtgaga tctcgaattc
     6901 ctggaagtgg tcgcgcacgt cgatctcagt cgcgcgcgcg gcggaggcgc tggcggagcc
     6961 agaggaagta gaccgaccag atcacgacga ccgcgatgta ggcgatgatc gcgtagttca
     7021 tgccgagacc ctcccgccgg cggggccggc cggatctctg gggtcgcgtc cgtgccgggc
     7081 caaccccggc gccatctcgc tctcccggtc catcgcggct tcgcgggccc actccagccg
     7141 gagccgcgtg acgaacgccg cggccaaaaa gacgatccag gcgagcatcg actcgagcag
     7201 cgtggcgcgc atgtcccccg tcatggtgac cttctgcgga tgcagcgacc cgcgccacca
     7261 ctggatggac atccagacga tcgggagatt cacgaacagc acgatgccga ggatcgagga
     7321 caccgtgcgc ttcgcgcccg aatgcaacga cgcccgcacc acgtggtagc ccgcgtacag
     7381 gatccagagg atcaggaacg tggtgaggcg aggctcccag gtccaccagg tgttccaggc
     7441 cgagcgggcc cagagcggcc cggtcaccat cacgacggtc gcgaacacca gccccagctc
     7501 gcacgccgaa gccgccatcg cgtcccagac ccagctcttc gtcgtcaggt aagcgatgct
     7561 gcaggcgaac gtgaacccga acgcgagcag ggtcgcgatc gcgctcggca cgtggacgta
     7621 gacgatcttc tgcaccggcc ccatctgtac ctcgatgggg gccttggaga ggatcatcca
     7681 ggcggacacc gcgaggagca ggaaggccgc cccccacaac gcgcgcccca ggggcctcca
     7741 ccaggacgga tagctcacgt tcatgtgcct gcctccacca cggatcgatt cattcgctcg
     7801 cctcctgaat cagctcgtac aggaacgccg ccagggccag ccccagcacg tccgacaccg
     7861 ccatcacctt cagccaatcc tggaccggcc ccgcggttcc ctccgcccag agggagtgcg
     7921 tgatcttgac ggcgccgatc cagagcggga ccaacgacgg aaagagcagg accgagagca
     7981 ggagctcgcg cgccttgagt ccccgcgcca gcgcggcgaa gagggtgccc cacgcagcat
     8041 acccgaccac ccccagcgcg gcaacgccga gcaccccggg cagcacgcgc aggttgccca
     8101 cgtcgaagaa gaccgacacg atcgggagca ccaggacgaa gacgccgccc aggatgagga
     8161 tgttggcgat cgtcttcccc aggtagatcg cgtaggggtc ggcgccggtc aggcgcatcg
     8221 cgtcgccgcc ccccgattcg gcctccaaat ccagcgagcg cgtcagtccc agcacgccgg
     8281 caaagaagag cgcggcccag agcgtcgcgc tggcctcgcg cgccgggtcc ttgaagatcg
     8341 gcccgaaggc cacgctcagg atcagcacga gcgaggcgct gaagagaagg agcgtcgcca
     8401 cgaagcgcgc gtcgcgcgcg tagagccgaa ggtctttcag cgcgaccgca aggaagctca
     8461 cgccgccacc ctgccccgga cgccggtccg cccgctcatg ccgtccccca atccaaacgg
     8521 tgcgctcaag ccccatcctc cacgacccgc ccctcctgca tcaggcagag ccgggttcgg
     8581 agcgcgccca accgctcgcg atcgtgggag atgatcatcg tcgccgcacc ccggtcgcgc
     8641 gcttcgccga tcagcccgtt gagctcggca gtgccgtcgt cgtcgagcgc ggtgtagggc
     8701 tcgtccagca gcaagagccg cggctccccc aggaaggcgc gggccagcga cacgcgctgg
     8761 agcatcccgc gggagaagcg ctcgacgggt gttttggcaa aggcgcccat gccgaaccgc
     8821 tccagaagct ccagggcgcg ctggtcggga tggcgcaccc cggtcagctt tccgaagagg
     8881 ctcaggttct ccagaggggt cagtcccagg tacaagagcg ggcggtgcga ggcgaagccc
     8941 atggcccgcc gcgcctcgcg
//
LOCUS       DASSLZ010000156         7409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1060308, whole genome shotgun sequence.
ACCESSION   DASSLZ010000156 DASSLZ010000000
VERSION     DASSLZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060308"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFM00_06120"
     CDS             complement(<1..581)
                     /locus_tag="VFM00_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497856.1"
                     /translation="MNSAVRVLGIAITAAALLLLVSPAHAQYLWLDTNGDGVNTVADF
                     LLTDGTPTAVDAWINTSHNKNGSTATCTSGEPDILLQTWNSFAIHLNVMNGTATFAGY
                     VNHIPSFGIACAAAGAGFLTNGSTEMSVCQASGTPMGNGGVNQKMFTVTVTVTSGTPS
                     LEFVPANSLDMNPTSFGSACSGMDFDNTIKLGS"
     gene            complement(839..2071)
                     /locus_tag="VFM00_06125"
     CDS             complement(839..2071)
                     /locus_tag="VFM00_06125"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7497857.1"
                     /translation="MPSTRPPRTPGSGSRVVLFFTVFIHLLGFGIIIPLLPYYAEHFG
                     ASGLTVGLLTTSFSLAQFLFAPIWGRLSDRIGRRPVLLGSLILTGVSYLVFAVATSLP
                     VLFLSRIFAGIAGATLSTAQAYIADTTTAENRTKGMGMIGAAFGMGFIFGPAMGGVLS
                     KWGFSVPAYAAAGLATVAFLFAMFRLRESLPPEEREAAARRRENAATFREALARPVVG
                     LVLVLFFVTTLCFSGMEAILALFCERFFAWGPHQIGYLFAYVGVVAAVMQAGIVGALA
                     KRFGERALVRAGLLLMGAAFITAGIVPPLAIHDPTHVVGGVLATFFAVMGVIAIGSGL
                     TTPSLSSLVSLATPADEQGGILGVYQSLGSLGRATGPFLGGLVFDVVSPGAPLWIAGS
                     VMVVASFVAARISLRRDT"
     gene            2147..4354
                     /locus_tag="VFM00_06130"
     CDS             2147..4354
                     /locus_tag="VFM00_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7497858.1"
                     /translation="MNFRPRAFLLALLFLGAAPAAGAEEALVLSGGGARGLAHAGVVL
                     GVERRGHDPGIVVGTSMGSIVGGLYAAGYTGAEIRAILVQQDWREIFTPFPFEVGPWR
                     DVRYPVVRLDTSTGAAFGTRAAIPDWRINRQLVRFLFEPGARAAGSFDRLPRRFRSVA
                     ADAETGEMISIGSGDLARAVRASMAAPGFFAPIRWPRPDGRGGPSAGTGRNLTDGGLA
                     DYLPVAEARRLGGDWVVASDVMVPNHPLESGDALAIARRSTELLTVNARREKTPPDVL
                     VIPEIDPGLEEFHYPSEPDPVIEGGLRTALRVLPPAADSATPSAGDSATPSAGGSTTP
                     PPARAPGTNRTPRPLPASLASLVIEPASAATPDERLSGFLRRAFGRVAPGAFHPDQVL
                     AVVDRLYTTGLFDGIWPSVEPADTSAPDAPLVLRVRSEPRPLLSVSGSLGYDNDRGGR
                     IWGSVRRLEALGSVPYDISLSGSANGVEQWGAAEVHLTSLWLGASAWTLGASFGESEL
                     RFARAEADGHLEVERASVWGGFETRSLRTGLQASVGVRTEQINADFGPEGGSAGPWVR
                     LRRIAPLVEALGGSTEIGAETRFGDVEYRRAWAKGGIAGRWHGVEAALAGDAAVVGGE
                     APLDAAPSMGGEGLVPGLREGERRGRARAVAGADLAIHTKPVALLRLRARGGVIADEC
                     RPERGIPYSREKLWLAGVRLSGIWWTVFGRLEIGADASTLGDRRAIIDLGTQF"
     gene            4354..5088
                     /locus_tag="VFM00_06135"
     CDS             4354..5088
                     /locus_tag="VFM00_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016914875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET7497859.1"
                     /translation="MPNPPARHRAVAPEPLSRSFYARSALTVARELLGCVLARRLPDG
                     AVLRGRIVETEAYVGEEDKACHARAGRTARTDPLYGPPGLAYVYITYGMHYMLNAVAE
                     PEGRPAAVLIRAAEPVEGIEWMVSARGSGAKTLTARAAAARHIAAGPARLTQAFGIDL
                     RQNRADLRGPEVWIEPGSPVPDRQVGTSARIGCQTAPAPWDAIPWRFYVAGNPHVTPG
                     TPRGAVTPSGNASKRRKTGGGARERH"
     gene            5236..6504
                     /locus_tag="VFM00_06140"
     CDS             5236..6504
                     /locus_tag="VFM00_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7497860.1"
                     /translation="MSGILTEQNLMWRARAREVAEKSVRPLAAKYDREQEYPWEIKQA
                     IADAGLMGVWIPKEYGGHGGGVLDLCICVEELSRACGGVGVLYAVNALGTFPILVGGT
                     EEQKQKYLRPIAEGKKLISFGLSEKFAGSDAASLHATAVKDGDDYILNGEKKWNTNGG
                     AADVYSVFAVTQPDSKSRRISAFIVEKGMPGFRIGKVEDKMGIRCVPVVEIHFDNCRV
                     PAKNLLGEVAGHGFKHAMMTLDKARPGVASQAVGLAQGALEYATVYAGHREQFGSPII
                     SFQMIQGMLADMATRVEAARQLVYAAARAVDEGAPNVSKLAAMCKLFATDTAMQVTTD
                     AVQIFGGYGYMRDYPIEKYMRDAKITQIYEGTNQVQRMVVARSLIKEALGLKFLLDYI
                     PKETQKGVNDPETLARKKDETRETVESDKA"
     gene            6533..7018
                     /locus_tag="VFM00_06145"
     CDS             6533..7018
                     /locus_tag="VFM00_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7497861.1"
                     /translation="MQTTMDRTETLKVGDVAPDFTLKDQDERDVTLSSFRGKKNVVLI
                     FHPLAFTSICSVQMPGYNKELQTFEGMDTQVLGLSVDSSPAHRAWAEMLGGIDYPMLA
                     DFHPHGEVSKRYGILRPEGFSERATIVIDKKGIVRSIEVHDIGTLPDHDKLLETLRSI
                     R"
     gene            7015..>7409
                     /locus_tag="VFM00_06150"
     CDS             7015..>7409
                     /locus_tag="VFM00_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497862.1"
                     /translation="MRRPGTWALLGVVVAAASMIAASVPAARKASATPVRQRPVTSRP
                     AAKMPLPPRAMGQADAKAAAVAKQLSDAMGGQRTWDVLPYFRFDFVVVQKGKEVARFQ
                     HWWDKRNGQCRVQGPDDKGRTVTAIFTLK"
BASE COUNT         1202 a         2513 c         2608 g         1086 t
ORIGIN      
        1 tccgacccca gcttgatggt gttgtcgaaa tccattcccg agcaggcgct gccgaacgag
       61 gttggattca tgtcgagact gttcgcggga acgaactcga gcgaaggcgt gcccgacgtg
      121 accgtcacgg tgaccgtgaa catcttctgg ttcacgcctc cgttacccat cggggtgccg
      181 gatgcctgac aaacactcat ctcggtgctg ccattggtaa ggaaccccgc gccagcagcg
      241 gcgcaggcga ttccgaacga tgggatgtga ttcacgtagc ctgcgaaggt tgctgttccg
      301 ttcatcacgt tgagatggat cgcgaacgag ttccaggtct gcagcagaat gtccggctct
      361 cccgaggtgc acgttgccgt ggagccgttc ttgttgtggc tcgtattgat ccaggcatcg
      421 acggcggtgg gcgtcccgtc cgtgagcagg aaatccgcga ccgtgttcac gccgtcgccg
      481 ttggtgtcca gccagaggta ttgcgcgtga gcgggggaga cgaggaggag cagtgccgcg
      541 gccgtgatcg cgatcccgag aacccgaacg gcggaattca tgatgtcagc tcccccctgg
      601 aggaatgcgc gaacggcccc gagggcggca agtcgcctcg acgcgtaagc ggatcgtgtc
      661 ggaccgaggg tggtccaaag agagaacgcg ggtgcggggc aggaacgcac gactccccct
      721 cggtgggtgg tgcgcggcag tgggggaggg gccgccgcga aagagcggtc agctttgtga
      781 ttatgttaac aaataccggg ataagtagca atcccaaaat gacttcacag cttgtgcgct
      841 acgtatccct ccgtaacgaa atccgtgcgg ccacgaacga cgcgacgacc atgaccgacc
      901 ccgcgatcca gagcggcgcg ccggggctga cgacgtcgaa tacgagtccg cccaggaacg
      961 gtccggtggc gcggcccagg cttcccagcg attggtacac gccgaggatc ccgccctgct
     1021 cgtccgccgg cgtggcgagc gacacgaggg aggagaggga gggtgtggta aggcccgatc
     1081 cgatcgcgat cacgcccatg acggcgaaaa aggtggccag cacgccgccc acgacgtgcg
     1141 tcgggtcatg gatcgcgagc gggggaacga tgcccgcggt gatgaacgcc gcgcccatca
     1201 gcaacaaccc cgcgcgcacc agggcccgct cgccgaagcg cttggccagg gcaccgacga
     1261 tccccgcctg catgaccgcg gccacgactc cgacgtaggc gaacagatag ccgatctggt
     1321 gcggccccca ggcgaagaag cgctcgcaga agagggcgag gatcgcttcc attcccgaga
     1381 agcagagggt ggtcacgaag aagaggacca gcacgagccc gacgaccggc cgcgccaagg
     1441 cctcgcggaa ggtcgcggcg ttctcgcgcc ggcgcgccgc ggcctcgcgc tcctccggag
     1501 gcagcgattc gcggaggcgg aacatcgcga acaggaacgc gaccgtggcg agccccgccg
     1561 cggcgtacgc cggcaccgag aacccccact tgctcagcac gccgcccatg gccggtccga
     1621 agatgaaccc catgccgaag gcggcgccga tcatccccat ccccttcgtg cggttctcgg
     1681 cggtggtggt gtcggcgatg taggcctgcg cggtggagag ggttgccccc gcgatccccg
     1741 cgaagatgcg cgagaggaac aggacgggca gggaggtcgc gacggcgaag accaggtagg
     1801 agacgccggt caggatgagc gagccgagga gcaccggccg gcgtccgatc ctgtcggaga
     1861 gccggcccca gatcggggcg aagaggaact gcgccagcga gaaggaggtg gtgagtagcc
     1921 cgaccgtgag cccgctcgcc ccgaagtgct ccgcgtaata ggggaggagc gggatgatga
     1981 tcccgaaccc gaggagatgg atgaagacgg tgaagaagag aacgacgcgc gagccggagc
     2041 cgggagttcg tgggggacgg gtcgaaggca ccgacggatt ctagggaggg cgaggcagcg
     2101 ggtcaattga ctgactgggt cagtcaccct gttgcatact gctcgcgtga atttccgccc
     2161 gcgggctttc ctgttggcct tgctcttcct cggtgccgcg cctgccgccg gcgcggagga
     2221 ggcccttgtt ctttccggtg gcggcgcgcg cggactcgcg cacgccggcg tggtgctcgg
     2281 cgtggagcgg cgcgggcacg atcccggcat cgtggtcgga acgagcatgg ggtcgatcgt
     2341 gggcggcctc tatgccgcgg gatataccgg cgccgagatc cgcgcgatcc tggttcagca
     2401 ggactggcgc gagatcttca cgccgtttcc gttcgaagtc gggccatggc gcgatgtccg
     2461 ctacccggtc gtgcgcctgg acacctccac gggcgccgcg ttcggcaccc gcgcggcgat
     2521 ccccgactgg cggatcaacc gccagcttgt ccggtttctg ttcgagcccg gcgcccgcgc
     2581 ggcgggcagc ttcgatcggc tgccgcggcg gttccgctcg gtcgcggcgg acgccgagac
     2641 gggggagatg atctcgatcg gctccggaga tctcgcgcgc gcggtgcggg ccagcatggc
     2701 agcgcccggt ttcttcgcgc cgattcgatg gccgcgcccg gacggacggg gtggcccttc
     2761 ggccggaacg ggccgcaacc tcaccgatgg cggtctcgcc gactatctcc cggtggccga
     2821 agcgcggagg ctcgggggcg actgggtcgt cgcatcggac gtgatggtcc cgaaccaccc
     2881 gctcgagagc ggagatgcgc tcgcgatcgc gcggcgcagc acggagctct tgaccgtgaa
     2941 cgcgaggcgg gagaaaaccc cgcccgatgt gctggtcatt cccgagatcg atcccggcct
     3001 ggaggagttt cactatccct cggagccgga tccggtcatc gagggcggtc tccgcacggc
     3061 gcttcgggtg cttcctcccg cggccgactc ggccacgccg tccgccggcg actcggccac
     3121 gccgtccgcg ggcggctcga ccacgccgcc acccgcccgc gcgccgggta ccaaccgcac
     3181 gccgcgacct cttccggcct cgctcgcctc gctcgtgatc gagccggcct ccgccgcgac
     3241 acccgatgag cgtctgagcg gatttctccg ccgcgcgttc ggccgcgtcg caccgggcgc
     3301 attccatccc gatcaggtcc tcgccgtcgt ggaccgcctg tacacgacgg gcctgttcga
     3361 tggaatctgg ccctcggtcg agcccgccga tacgtcggcg cctgacgcgc ccctcgtcct
     3421 ccgggtgcgc tcggagccca ggccgctctt gtccgtctcc ggctcgctcg ggtacgacaa
     3481 cgaccgcggc gggcggatct ggggatcggt acggcggctc gaggcgctcg gttcggttcc
     3541 gtacgacatc tcgctctcgg gatcggcgaa cggcgtcgag cagtggggcg ccgccgaagt
     3601 acacctcacc tctctctggc tcggcgcttc ggcgtggacg ctgggcgcga gcttcggcga
     3661 atccgagctt cgtttcgcgc gcgccgaggc cgatggccac ctcgaagtgg agcgcgcgag
     3721 cgtctggggc ggattcgaga cgcgctccct gcgcacgggc ctccaggcgt cggtgggcgt
     3781 ccgcacggag cagatcaacg ccgacttcgg ccccgaaggc ggctccgccg gtccctgggt
     3841 ccggctccgc cgcatcgcgc cgctcgtgga ggcgctcgga gggtccacgg agatcggcgc
     3901 cgagacccgg ttcggcgacg tggagtaccg cagggcgtgg gccaagggtg ggatcgccgg
     3961 ccggtggcac ggtgtcgaag ccgccctggc cggagacgcg gccgtggtcg gcggggaggc
     4021 gccgctcgac gcggcgccct cgatgggggg cgaggggctg gtgccggggc tgcgcgaggg
     4081 ggagcggcgt ggccgcgccc gggcggtggc cggagccgat ctcgcgatcc acacgaagcc
     4141 ggtcgccttg cttcggctgc gcgcgcgcgg cggcgtgatc gccgacgagt gccgtccgga
     4201 gcgaggcatt ccctactcgc gggagaagct ctggctggcg ggcgtccgac tctcgggaat
     4261 ctggtggacc gtgttcggtc gcctggaaat cggcgccgac gcgagcacct tgggcgaccg
     4321 tcgcgcgatc atcgacctgg ggacccagtt ctgatgccca acccgcccgc acgtcaccgc
     4381 gccgtcgcgc ccgagccgct ctcgcgctcc ttctacgcgc gctccgccct caccgtcgcg
     4441 cgcgagctcc tcggctgcgt cctggcgcgc cggcttcccg acggcgccgt ccttcgcggg
     4501 cgcatcgtcg agaccgaggc gtacgtcggc gaggaggaca aggcctgcca tgcgcgcgcg
     4561 gggcgcacgg cccgcaccga tccgctctat gggccgcccg gtctcgccta cgtctacatc
     4621 acctacggga tgcactacat gctgaacgcc gtcgcggagc cggagggaag gcccgccgcg
     4681 gtcctgatcc gtgccgccga gccggtcgag gggatcgagt ggatggtgag cgcgcgcggc
     4741 tcgggggcga agaccctcac cgcccgcgcc gccgcggcgc gccacatcgc ggcggggccc
     4801 gcccgcctga cccaggcgtt cgggatcgat ctgcgccaga accgggccga cctgcgcggg
     4861 ccggaggtct ggatcgagcc gggttcgccc gttcccgacc ggcaggtcgg cacgtcggcg
     4921 cgcatcggct gccagacggc gcccgctccc tgggacgcca tcccctggcg gttctacgtg
     4981 gcgggaaacc cgcacgtcac gccgggcacc ccccgcggag ccgtaacccc ttcgggaaac
     5041 gcctcgaagc ggcgcaaaac tgggggtggc gcgcgggagc ggcattgagg ataatcggcc
     5101 tcttggaggc gccctgagcc cgcttgggct ccgacaccac ggcgccgccg tcaccgcggg
     5161 ttccgccgca cgcgcgctcg gcgagccgcc cgcacgatcg ggaccgtcgc aacgcaactg
     5221 gaggggggca cctccttgtc cggcatcctc accgaacaga atctgatgtg gcgggcgcgc
     5281 gcccgggaag tggccgagaa atcggtccgt ccgctcgcgg ccaagtacga ccgggaacag
     5341 gaatatccgt gggagatcaa gcaggccatc gcggacgcgg ggctcatggg ggtctggatc
     5401 cccaaggagt acggcggcca cggcggcggc gtgctcgatc tctgcatctg cgtcgaggaa
     5461 ttgtcgcgtg cctgcggcgg cgtcggcgtg ctctacgccg tgaacgcgct gggcaccttc
     5521 ccgatcctgg tcgggggcac cgaggagcag aagcagaagt accttcgccc catcgccgag
     5581 gggaagaagc tcatctcctt cggcctctcc gagaagttcg ccggcagcga tgccgccagc
     5641 ctccacgcca ccgcggtcaa ggacggcgac gactacatcc tgaacggcga gaagaagtgg
     5701 aacaccaacg gcggcgcggc cgacgtctac tccgtcttcg ccgtgacgca gcccgacagc
     5761 aagtcgcggc gcatcagcgc cttcatcgtc gagaagggga tgcccggctt ccggatcggc
     5821 aaggtcgagg acaagatggg gatccgctgc gttcccgtgg tggagatcca cttcgacaac
     5881 tgccgggtcc cggcgaagaa cctcctcggc gaagtcgcgg ggcacggctt caagcacgcc
     5941 atgatgacgc tggacaaggc acgtccggga gtcgcctcgc aggcggtggg actggcgcag
     6001 ggcgccctgg aatacgcgac cgtctacgcc gggcaccgcg agcagttcgg ctcgccgatc
     6061 atctccttcc agatgatcca gggaatgctg gccgacatgg ccacgcgcgt cgaggcggcg
     6121 cggcagctcg tctacgccgc ggcccgcgcg gtggacgagg gcgcgcccaa cgtctccaag
     6181 ctcgccgcca tgtgcaagct gttcgccacc gataccgcga tgcaggtcac gaccgacgcc
     6241 gtccagatct tcggcggcta cggatacatg cgcgactatc ccatcgagaa gtacatgcgc
     6301 gacgccaaga tcacccagat ctacgaaggc accaaccagg tgcagcgcat ggtcgtcgcc
     6361 cgctcgctga tcaaggaggc gctcgggctc aagttccttc tcgactacat tcccaaggag
     6421 acgcaaaagg gcgtgaacga ccccgagacg ctcgcgcgga agaaggacga gacgcgcgaa
     6481 accgtggagt cggacaaggc ctgacgtctc ctgtcgccct caaggagacc gcatgcaaac
     6541 gacgatggac cggaccgaga cgctcaaggt cggcgatgtc gcgcccgact tcacgctgaa
     6601 ggaccaggac gagcgcgacg tcacgctctc ctcgttccgc gggaagaaga acgtcgtgct
     6661 catcttccat ccgctcgcct tcacgtcgat ctgctcggtc cagatgcctg gctacaacaa
     6721 ggagctccag accttcgagg ggatggacac gcaggtgctc ggcctctcgg tggattcctc
     6781 gccggcgcat cgcgcctggg cggagatgct gggcggcatc gattacccca tgctcgccga
     6841 cttccatccg cacggcgaag tctcgaagcg ctacgggatc ctccgtcccg agggcttttc
     6901 ggagcgcgcg accatcgtga tcgacaagaa ggggatcgtc cgcagcatcg aggtccacga
     6961 catcggcacc ctcccggacc acgacaagct gctcgagacg cttcgctcca tccgatgaga
     7021 cggcccggca cgtgggcgct cctgggagtc gtggtcgccg cggcttcaat gatcgcggcc
     7081 tccgtgccgg ctgcccggaa ggcgtccgcc actcctgtcc ggcagcgccc ggtcacctcc
     7141 agacccgcgg cgaagatgcc gctccctccg cgcgccatgg gccaagccga cgcgaaagct
     7201 gcggccgtcg ccaagcagct gagcgacgcg atgggcgggc agcgcacctg ggacgtgctc
     7261 ccttatttcc gcttcgactt cgtggtcgtg cagaagggga aagaggtcgc ccggttccaa
     7321 cactggtggg acaagcgcaa tggccagtgc cgcgtccaag ggccggacga caaggggcgc
     7381 accgtcaccg cgatcttcac gctgaagga
//
LOCUS       DASSLZ010000157         4363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_168915, whole genome shotgun sequence.
ACCESSION   DASSLZ010000157 DASSLZ010000000
VERSION     DASSLZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168915"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VFM00_06155"
     CDS             <1..1120
                     /locus_tag="VFM00_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7497863.1"
                     /translation="FADKEIAPTASKNDAAGEFPWDNVKKMRGLGLFGLIFPEQYGGS
                     GAETLMYVMAVEEVSRACASTGITLAAHVSLGTAPIYLFGTEAQKRKYLPKLCSGENL
                     GAFGLTEPEAGSDAGGTKTRAVKEGDQYAVNGRKIYITNGGVCGTAVFTAVTAPGFGA
                     RGISAFIVEKGTPGFSAGPPEKKLGHRASNTVELIFENLRLPKENLLDQDGREGNGFK
                     QFMITLDGGRISIGAMSLGIAQAALDSAVRYAMNRRQFGKAIGDFQAIQLLLAEMATE
                     LESARLLVYAAARLKDRGAPFTRYAAMAKLKASTVAMRAADAAVQIHGGAGYMTDHPV
                     ERYFRDAKLMEIGEGTSQIQKLLIARELLKEAGHVFGS"
     gene            1185..2375
                     /locus_tag="VFM00_06160"
     CDS             1185..2375
                     /locus_tag="VFM00_06160"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET7497864.1"
                     /translation="MVAERDAYIVSACRTAIGEFLGGLSTMTAPQLGAVAIREAVQRA
                     GIEPGQVEEVLMGHVVQAGAGQAPARQAAIHGGIPDTVGATTINKVCGSGLKAVMLAA
                     QAIKAGDADVIVAGGMESMSNVPYLLPKARTGYRLGNGELVDGVVFDGLWCAFKNHHM
                     GNSAEFIAKKFSVTREDQDRFSVDSHKKAVAAQKEGAFKDEIVPVLIPQKKGEPKCFC
                     QDERPRPDTSMETLGKLRPAFDKEGTVTAGNAPGLNDGASAVVVMSEAALKRTGAKAM
                     ARINGYATGGVAPEMIFYAPVIAVRKLAEKLGRKTNEWDLIEANEAFAAQAIVDAKEL
                     NWDPARLNVRGGAVALGHPIGASGARVLTTLLYAMRDKKAKTGLATLCLGGGNAVALS
                     VEAV"
     gene            2372..3274
                     /locus_tag="VFM00_06165"
     CDS             2372..3274
                     /locus_tag="VFM00_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010531552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET7497865.1"
                     /translation="MTSASAPTGAASKSPTITARPIVVAGAGTMGNGIAQTFAASGHR
                     VLLLEANPAALQRGMGTITKSLGKLVEKGKLTMEARDEAIGRIATEAASFPTGDCVIA
                     VEAVPEKLEIKREVLRMLDGMLPAGAILATNTSSLSITELGAGTSRAARFVGMHFMNP
                     VPLMPLVEVVRGMDTSDATVTETVELAKALGKTPVVVSDRPGFVSNRVLLPMINEAAF
                     ALMEGVADREAIDTVMKLGMNHPMGPLALADLIGIDVCLDIMEVLHQGFGDSKYRPCP
                     LLKSMVAAGRLGRKTGRGFYDYAT"
     gene            3290..>4363
                     /locus_tag="VFM00_06170"
     CDS             3290..>4363
                     /locus_tag="VFM00_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7497866.1"
                     /translation="MNFEPTEEQEMLRSFAREFAQGEIVPRAHEIETSATLPEELRQA
                     LRENNFFSLLIPEEFGGSAVGYVSYAMILEELARASAAVAITVSVHNSVTAGPIQTFG
                     TEVQRKKWLPLLAQTHLGAFALTEPGSGSDAAALTTRACRNGESYVLNGQKTFVTNGK
                     YADLFLVMARTSPDQKHRGISSFLVERDSPGLRIGKEIQKMGLHGSDTVELFFEDCKV
                     PAENRLAEEGYGFRVAMTSLDAGRIGVAAQALGIAQACLEAAVRYARERVQFGKPIAE
                     FQAIQWMVADMKAEIDGARLLTMRAAWRKDHGLPHGPESSIAKLTASAAACHCADKAV
                     QIHGGYGYTKEFPVERYYRDAKVT"
BASE COUNT          757 a         1455 c         1533 g          618 t
ORIGIN      
        1 gttcgccgac aaggagatcg cgcccacggc gtccaagaac gacgcggcgg gcgagttccc
       61 ctgggacaac gtgaagaaga tgcgggggct ggggctgttc ggcctcatct tccccgagca
      121 gtacggcggg agcggcgccg agaccctcat gtacgtcatg gcggtcgaag aggtgagccg
      181 cgcctgcgct tcgaccggca tcaccctcgc ggcgcacgtc tcgctgggca ccgcgccgat
      241 ctatctcttc gggaccgagg cgcagaagcg gaagtacctc ccgaagctct gctccggcga
      301 gaacctgggc gcgttcgggc tcaccgagcc cgaggccggg tcggacgccg gcggcacgaa
      361 gacgcgggcg gtcaaggaag gcgatcagta cgccgtcaac ggccggaaga tctacatcac
      421 gaacggcggc gtctgcggca ccgccgtgtt caccgcggtc accgcgcccg ggttcggcgc
      481 gcggggcatc tcggcgttca tcgtggagaa gggcacgccc gggttctcgg cgggcccgcc
      541 cgagaagaag ctgggacacc gcgccagcaa caccgtggag ctgatcttcg agaacctccg
      601 gcttccgaaa gagaacctgc tcgaccagga cggccgcgag gggaacggct tcaagcagtt
      661 catgatcacc ttggacggcg gacgcatctc gatcggcgcc atgtcgctcg ggatcgcgca
      721 ggccgccctg gattcggcgg tccgctacgc catgaaccgc cgccagttcg gcaaggccat
      781 cggcgacttc caggcgattc agctcctcct ggccgagatg gccacggagc tggaatcggc
      841 gcggctcctg gtctatgccg cggcgcgtct gaaagaccgg ggcgccccgt tcacccgcta
      901 cgccgccatg gccaagctca aggcatcgac cgtcgcgatg cgcgcggcgg acgcggccgt
      961 ccagatccac ggcggcgccg gctacatgac cgaccatccg gtggaacgct atttccgcga
     1021 cgcgaagctg atggagatcg gcgagggcac ctcgcagatc cagaagctcc tgatcgctcg
     1081 cgagcttttg aaagaggcgg gacacgtctt cgggagttga gggggggagc tgatcccccg
     1141 gcctgacctc ccggctcatc gaggacgccg ccgaaaggag agcaatcgtg gctgagagag
     1201 acgcctacat cgtcagcgcc tgccggaccg ccatcggcga gttcctcggc gggctttcca
     1261 ccatgaccgc gccgcagctc ggggcggtgg caatccggga agccgttcag cgcgccggca
     1321 tcgagccggg ccaggtcgag gaagtgctca tgggacacgt ggtgcaggcg ggcgccggac
     1381 aggctcccgc gcgccaggcc gcgatccacg gcggcattcc cgacaccgtg ggcgcgacca
     1441 cgatcaacaa ggtctgcggc tcggggctca aggcggtcat gctcgccgcg caggcgatca
     1501 aggccggaga cgccgacgtg atcgtcgcgg gcggcatgga gtcgatgtcg aacgtgccct
     1561 acctgctgcc gaaagcgcgc accggctatc gcctgggcaa cggcgagctg gtggacggcg
     1621 tggtgttcga tggcctctgg tgcgcgttca agaaccacca catggggaac tcggccgagt
     1681 tcatcgcgaa gaagttcagc gtcacccgcg aggaccagga ccgcttctcg gtggacagcc
     1741 acaagaaggc ggtcgccgcc cagaaggaag gcgcgttcaa ggacgagatc gtcccggtgc
     1801 tgatccccca gaagaaaggc gagcccaagt gcttctgcca ggacgagcgg ccgcgccccg
     1861 acacgtcgat ggagaccttg gggaagctcc gccccgcctt cgacaaggaa ggcaccgtca
     1921 cggcggggaa cgccccgggc ctgaatgacg gcgcctcggc ggtcgtcgtg atgtccgaag
     1981 ccgcgctgaa gcgcaccggg gccaaggcga tggcgcgcat caacggctac gccacgggcg
     2041 gcgtcgctcc cgagatgatc ttctacgccc ccgtgatcgc ggtgcggaag ctggccgaga
     2101 agctcggccg gaaaacgaac gagtgggacc tgatcgaggc gaacgaggcg ttcgcggcgc
     2161 aggcgatcgt ggacgcgaag gagctcaact gggatccggc gcggctcaac gtccgcggcg
     2221 gcgcggtggc gctggggcat ccgatcggag cctcgggcgc gcgcgtcctc acgacgctcc
     2281 tctacgccat gcgcgacaag aaggccaaga ccggtctcgc gaccctctgc ctgggcggcg
     2341 gcaacgccgt ggcgctcagc gtggaggccg tatgacctca gcttcggcgc cgacaggcgc
     2401 cgccagcaag agcccaacca tcacggcgcg ccccatcgtt gtcgcaggcg ccggtacgat
     2461 gggaaacggc atcgcccaga ccttcgcagc ctcgggacat cgcgtgcttc ttcttgaggc
     2521 gaaccccgcc gcgctccagc gcggcatggg aacgatcacc aagagcctcg ggaagctggt
     2581 ggagaagggg aagctgacga tggaagcgcg ggacgaagcc atcgggcgca tcgcgaccga
     2641 ggcggcctcg ttcccgacgg gcgactgcgt gatcgcggtc gaggcggtgc ccgagaagct
     2701 ggagatcaag cgcgaggtgc tccgcatgct cgacgggatg cttccggcgg gcgcgattct
     2761 cgccacgaac acctcttccc tctcgatcac cgagctgggg gccggcacca gccgtgccgc
     2821 ccgcttcgtc ggcatgcact tcatgaatcc ggtgcctctc atgccgctgg tcgaggtggt
     2881 gcgcgggatg gacacgagcg acgcgacggt gaccgagacc gtggagctcg ccaaggcgct
     2941 cggcaagacg cccgtcgtgg tctcggaccg gcccggtttc gtctccaatc gcgtgctcct
     3001 gccgatgatc aacgaggcgg cgttcgccct gatggagggg gtcgcggacc gggaagccat
     3061 cgacacggtg atgaagctgg ggatgaacca tccgatgggg ccgctcgcgc tggccgacct
     3121 catcggcatc gacgtctgcc tcgacatcat ggaggtgctc caccagggtt tcggcgacag
     3181 caagtaccgc ccctgcccgc tgctcaagag catggtggcc gccgggcggc tgggtcgaaa
     3241 gacgggacgg gggttctacg actacgcgac ctgacggccg cgcccgccga tgaatttcga
     3301 gcccaccgaa gagcaggaga tgctccggtc gttcgcgcgc gagttcgccc aaggcgagat
     3361 cgtgccgcgc gcgcacgaga tcgagacgtc cgcgaccttg ccggaggagc tccgccaggc
     3421 gctccgcgag aacaacttct tttccctgct gatccccgag gagttcggcg gctccgccgt
     3481 cgggtacgtc tcctacgcga tgatcctcga ggagctggcc cgcgcctcgg ccgccgtggc
     3541 gatcacggtc tcggtgcaca actccgtcac ggccgggccg atccagacgt tcggaaccga
     3601 ggtccagcgg aagaagtggc tcccgctcct ggcccaaacc catctgggcg ccttcgccct
     3661 gaccgagccc ggctcgggat ccgacgcggc cgcgctcacc acccgggcgt gccggaacgg
     3721 ggagagctac gtcctgaacg ggcagaagac cttcgtcacg aacgggaaat acgccgacct
     3781 gttcctggtg atggcgcgga ccagcccgga ccagaagcac cgggggatca gctccttcct
     3841 ggtcgagcgg gattcgccgg ggcttcggat cggcaaggag atccagaaga tggggctgca
     3901 cggctccgac acggtggagc tcttcttcga ggactgcaag gtccccgccg agaaccgcct
     3961 tgccgaggag gggtatggat ttcgagtggc catgacctcg ctggacgccg gccggatcgg
     4021 cgtcgccgcg caggcgctcg ggatcgccca ggcgtgcctg gaggccgccg tgcgctacgc
     4081 ccgggagcgc gtccagttcg ggaagccgat cgccgagttc caggcgatcc agtggatggt
     4141 ggccgacatg aaggcggaga tcgatggggc gcgcctcctc acgatgcgcg cggcctggcg
     4201 caaggaccat gggctgccgc acggccccga gtcctcgatc gccaagctga ccgcctcggc
     4261 ggccgcctgc cactgcgccg acaaggcggt tcagatccac gggggctacg gctacaccaa
     4321 ggaattcccg gtggagcgct attatcggga cgccaaggtg acc
//
LOCUS       DASSLZ010000158         3700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_169244, whole genome shotgun sequence.
ACCESSION   DASSLZ010000158 DASSLZ010000000
VERSION     DASSLZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169244"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFM00_06175"
     CDS             <1..672
                     /locus_tag="VFM00_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HET7497867.1"
                     /translation="LVAGSWTAARAAADAAVSAASLLSSGARAAFSAMRPPGHHAGPD
                     FMGGYCFLNNAAIAAEALRARGCDRVAILDVDYHHGNGTQSIFYARTDVLTVSVHGDP
                     RTEYPFFLGHEDEMGEGPGVGFNRNFTLPAGTGFESWFAALEAACARVREHRADALVV
                     PLGLDTYRGDPISKFLLESGDFSKVGARLASLKLPTLFVMEGGYATKELGVNACNVLE
                     GFEQG"
     gene            complement(707..2710)
                     /gene="ftsH"
                     /locus_tag="VFM00_06180"
     CDS             complement(707..2710)
                     /gene="ftsH"
                     /locus_tag="VFM00_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7497868.1"
                     /translation="MAKRTRRQVEGGDTARRPGEPPRPPRPPGSPTPPAIPPRRSWLF
                     FLLILLANFLLIRFFAPNPHTVKIPYSVFRLEAAKGNVLKINSRGEGLTGQFRTPISY
                     PAVADSATGAVPKPVRVFSTTLPIFTDSSLDTLLVARGVEVTAEPSQQGNLWLNLLFS
                     FAPALLFIGLYVWITRRVTKQGGGFGGGLMGLGRSTAKRFDTEAEGKVTFEDVAGIDE
                     AENELVEIVDFLKDPKKYTRLGGAAPKGVLLIGAPGTGKTLLARAVAGEAGVPFFSMS
                     GSEFVEMIVGVGAARVRDLFKMAREHAPAIIFVDELDSIGRARGQISIGGSSEQEQTL
                     NQILTEMDGFSSREGIIVLAATNQPDILDRALLRPGRFDRRVIVNLPDRKGREAILVV
                     HTRRVPLAPDVNLGEVAGATPGLAGADLRNLVNEAALLAARRGQNDVHQKDFLDALEK
                     IVLGPERPLLLSRADRERIAFHEGGHAILGLVVDGADPVHRVSIVPRGQALGVTYQRP
                     ESDRYNYPEAYLRARIVGMLGGRAAEEIVFGTKTTGAQNDIEQATELARSMVTRWGMS
                     ERLGMVQLAPRENPYLGLMDGYGGSRPYSEETASAVDAEVLRIIRESHEEAKRLLVKN
                     RSALDALAEALLARETLDEQEILEVTGLPPASPLSEKRPNPER"
     gene            2937..3332
                     /locus_tag="VFM00_06185"
     CDS             2937..3332
                     /locus_tag="VFM00_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7497869.1"
                     /translation="MKGFIGDIEELTDGNSNFRRVVYTGKQLQLVLMSLRPGEEIGEE
                     THRDRDQFFRIEKGRGEVWIDGHKTAIKGDFAIVVPAGAKHNIRNTGEKPMRLYTLYG
                     PPEHADGTIHATRAEAMASKEHFAGRTTE"
     gene            complement(3355..>3700)
                     /locus_tag="VFM00_06190"
     CDS             complement(3355..>3700)
                     /locus_tag="VFM00_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HET7497870.1"
                     /translation="LDLLAGGPGMRRGRRHPEHLLPGDALDWWRVETIEPDRLLRLAA
                     EMRLPGRAWLQYEVEPCAEGGAVIRQTALFDPLGLLGLAYWYALWPVHQYVFSGLLSQ
                     IARRAKATGTER"
BASE COUNT          564 a         1290 c         1272 g          574 t
ORIGIN      
        1 ctggtggcgg ggtcctggac ggccgcgcgc gcggccgccg acgcggcggt gagcgccgcg
       61 tcgctgcttt cctccggcgc ccgcgcggcc ttctccgcca tgcgcccgcc cggccatcac
      121 gcgggtcccg acttcatggg aggctactgc ttcctcaaca acgcggcgat cgccgccgag
      181 gccctgcgcg cgcgcggctg cgaccgggtc gcgatcctcg acgtcgacta tcatcacggc
      241 aacgggaccc agagcatctt ctacgcccgt accgacgtgc tcaccgtcag cgtccacggc
      301 gatccgcgca ccgagtaccc cttcttcctg ggacacgagg acgagatggg cgagggtccg
      361 ggggtcgggt tcaaccggaa cttcaccctc ccggccggca ccgggttcga atcgtggttc
      421 gccgcgctcg aagcggcctg tgcgcgcgta agggagcatc gtgccgacgc gctcgtggtt
      481 ccgctgggcc tcgacaccta ccgcggcgat cccatctcca agttcctcct cgagtcgggc
      541 gatttctcca aggtgggcgc gcgcctcgcg agcctgaaac tcccgacgct gttcgtgatg
      601 gaggggggct acgcgacgaa ggagctgggc gtgaacgcgt gcaacgttct cgaagggttc
      661 gagcagggct gagcgccgcg acggtcccgc cgtgcggcgg taggtctcag cgctccgggt
      721 tcggacgctt ctcggacagc ggcgaggcgg gcgggagtcc ggtcacttcg aggatctcct
      781 gctcgtccag cgtctcgcgc gcgagcagcg cctcggccag ggcgtccagc gccgagcggt
      841 tcttcacgag gagacgcttc gcctcctcat ggctctcccg gatgatccgg agcacctcgg
      901 cgtccaccgc gctcgccgtc tcctcgctgt agggcctgga cccgccgtag ccgtccatca
      961 ggcccaggta gggattctcc cgcggcgcca gctgcaccat ccccagccgc tcgctcatcc
     1021 cccagcgcgt caccatgctc cgcgccagct cggtcgcctg ctcgatgtcg ttctgcgccc
     1081 ccgtggtctt cgtcccgaaa acgatctcct cggcggcgcg cccgccgagc atgcccacga
     1141 tgcgcgcgcg caggtacgcc tcggggtagt tgtagcggtc gctctcgggg cgctggtagg
     1201 tgacgcccag cgcctggcct cgcgggacga tggagacgcg gtggaccgga tcggctccgt
     1261 ccacgaccag ccccaggatg gcgtggcccc cctcgtggaa ggcgatgcgt tcccggtcgg
     1321 cgcgggatag gagcagcggc cgctcgggcc ccagcacgat cttctccagc gcgtcgagaa
     1381 agtccttctg atgcacgtcg ttctgtccgc gccgggcggc gagcagcgcg gcctcgttca
     1441 ccaggttgcg gagatccgcc ccggccagcc ccggggtcgc cccggccacc tcccccaagt
     1501 tcacgtcggg cgcgagcgga acgcggcgcg tgtggacgac gaggatcgcc tcgcgcccct
     1561 tccggtccgg caggttcacg atgacgcggc ggtcgaaccg gcccgggcgc aggagcgccc
     1621 ggtcgaggat atcgggctga ttggtggccg cgaggacgat gatcccttcg cggctggaga
     1681 acccgtccat ctcggtcagg atctggttca gggtctgctc ctgctcgctg gagccgccga
     1741 tcgagatctg cccccgcgcg cgcccgatcg agtccaactc gtcgacgaag atgatcgccg
     1801 gggcgtgctc ccgcgccatc ttgaagaggt cgcgcacgcg ggccgccccc acgccgacga
     1861 tcatctccac gaattccgac ccgctcatcg agaagaaggg cacgcccgcc tcgcccgcca
     1921 cggcgcgcgc gagcagcgtc ttgccggtgc cgggcgcgcc gatcagcagt acccccttgg
     1981 gcgcggcccc gcccaggcgg gtgtacttct tcggatcttt caggaagtcg acgatctcga
     2041 ccagctcgtt ctcggcctcg tcgatgcccg cgacgtcctc gaacgtgacc ttgccttccg
     2101 cttccgtgtc gaaccgcttc gcggtgctcc ggcccaggcc catgagcccg cccccgaagc
     2161 cgccgccctg tttggtcacg cgccgggtga tccacacata gagcccgatg aagaggagcg
     2221 ccggcgcgaa gctgaagagg agattcagcc acaggtttcc ttgctggctc ggttcggcgg
     2281 ttacctccac gccccgcgcc acgagcaggg tatcgaggct ggagtcggtg aagatcggga
     2341 gcgtggtcga gaaaacgcgg accggcttcg gcacggcgcc ggtcgcggag tccgccacgg
     2401 cggggtagga gatgggcgtc cggaactgtc cggtcagccc ctcgccgcgc gagttgatct
     2461 tcaggacgtt gcccttcgcg gcttccagtc ggaagaccga atagggaatc ttgaccgtgt
     2521 gggggttggg agcgaagaag cggatcagca ggaaattggc gaggaggatc aggagaaaga
     2581 acagccagga gcgcctcggc gggatcgccg gcggcgtggg ggaccccggg ggcctcgggg
     2641 gccttggggg ctctcccggc cggcgcgccg tgtcaccgcc ctcgacttgc cgccgcgtcc
     2701 gcttcgccat cagaccgtcg atgctaccag caggaccggg tgcgaacaac ctgcccgcgt
     2761 gtcaggggct ctctgccaga ttgtccggaa cgaagacgcg aaccggccga cggagtccgc
     2821 gctccgcgtt gcctcccagc ggtatgcacc ccggcttccg gcggcacatg tgatgcaacc
     2881 cgatgtccat ggagcgaagc gtgtccggat gcgtcgtcta acaggggagg atcggcatga
     2941 aaggattcat cggcgacatc gaagagctca ccgacggcaa ttcgaacttc cgtcgcgtgg
     3001 tgtacaccgg caagcagctg cagctggtcc tgatgtccct tcgtccgggc gaggagatcg
     3061 gcgaggagac gcaccgggat cgcgatcagt tcttccgcat cgagaagggc cgcggcgagg
     3121 tctggatcga cggtcacaag accgcgatca agggagattt tgccatcgtg gtgcccgccg
     3181 gcgcgaagca caacattcgg aacacgggcg agaagccgat gcggctgtac acgctttatg
     3241 gcccgccgga gcacgccgac ggcaccattc acgcgacccg ggccgaggcc atggcgtcga
     3301 aggagcactt cgcggggcgc accacggagt aggaccctcc gtgcgtccgc gaggttaccg
     3361 ctccgtaccg gttgccttcg cgcgccgggc gatctgcgag agaagaccgg agaacacgta
     3421 ttgatgcacc ggccagaggg cgtaccagta cgcgaggccg agcagtccca ggggatcgaa
     3481 gagggcggtc tgccggatga cggcgccgcc ctcggcacat ggctcgacct cgtactgaag
     3541 ccacgcgcgt cccggcagac gcatctccgc cgccagccgg agcaggcggt cgggctcgat
     3601 cgtctcgacg cgccaccaat cgagcgcgtc cccgggaagg agatgctcgg ggtggcgtcg
     3661 tccgcggcgc atcccggggc ctcccgcaag cagatcgagc
//
LOCUS       DASSLZ010000159        12268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1447216, whole genome shotgun sequence.
ACCESSION   DASSLZ010000159 DASSLZ010000000
VERSION     DASSLZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12268
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447216"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="VFM00_06195"
     CDS             <1..1650
                     /locus_tag="VFM00_06195"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HET7497871.1"
                     /translation="PSGATFTANASNTSGTFNWTPSATQSGVYTVTFTAQNALSGSAS
                     TEITVNNVDAAPVVTAPATQSGPEGTTITFTVSATDPDGDVIVSLTGSPIPAGATFTV
                     TGEGTPTATGTFSWPNATPVGSYPITFTASNVLTGTATTVVNVTGVNHEPVVTAPATA
                     SGDEGTLITFTVSATDADGDHVTLTMLGGPSGATFSDNGDNTGTFSWTPGFNQAGVYT
                     VNFQGSDGNGATGSASTVITVNDVNRAPTADAGGPYTGVINVAVSFDGSGSSDPDGDT
                     LTYAWDFGDGGTGSGVNPSHTYTAGGTYTVTLTVTDPGTLSSTATTTATISTTLSANA
                     FTVGGNKTTSLGAGKPYTCVQVEAVDGSFQNTDVDISSIKMVSNGTGSVSEIFADPSK
                     SSVDGDKNGNGVPEIVACFKKSDLRLLFSNLAAGRQTVNVTIEGSLVSGATFSAPLTM
                     IVKSTGAVLVASVSPNPLNPQAKLTFATSKPGAVRVQMFDPQGRLVKTIADERAAMAG
                     YHDYTIDGRSSTGAKLASGVYFVKIVSEADGSTVTRVTIMK"
     gene            1853..2857
                     /locus_tag="VFM00_06200"
     CDS             1853..2857
                     /locus_tag="VFM00_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497872.1"
                     /translation="MKGLRRLTAWTLTLALFVLPSVRARAGDDVRRAYIGGAATLELQ
                     GSPVGFLRSASGGDLEGDVVTSAGPAVNKHISGIHYTDLVLEVGAGMEPAVYDWIAAA
                     WDAKATRGSGAVAVADYNFNVIARKEFQQGLITETSLPALDAASKDPAFLTVRVAPEL
                     VRITKGGGTVPSTLATKQRPWLANSFRLEIPGLDCTRVRRIEPLRIGTRIARDEVGNV
                     RTPVNQPTATSVSDLVITMPEAYADTWRDWVQQFLVQGQYNDSQEKNGDLVLLGPDMQ
                     SEIARVHLYGLGISSLKQARAESADAIRNVTATLYCERAALEWKGGGGAVMNVRVFGR
                     "
     gene            complement(2854..3438)
                     /locus_tag="VFM00_06205"
     CDS             complement(2854..3438)
                     /locus_tag="VFM00_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7497873.1"
                     /translation="MSRTILVLAVLSLLAASPALALPGEAGKVVQVGIGGGVSLPVSH
                     AKDALKNGFHVRGILEVKPPVFPLGLRGALGYQKFDLEHAPLGTTGSGNILSGMGGIT
                     IGMSLGPIRPYVTASLGAFRVETKMDSTGTETSAHETKFGIDGGAGVQFKLASMHGFV
                     EARIENVYTDQGFDTSFLEKNSTRIIPVTFGLMF"
     gene            complement(3534..6119)
                     /locus_tag="VFM00_06210"
     CDS             complement(3534..6119)
                     /locus_tag="VFM00_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET7497874.1"
                     /translation="MTNPGDGSVDGHLVVVGSSAGGIEALSVLASGLGKDFPAPVVFA
                     QHLDPSRPSQLAGILDRRTELTVSTVNGDTPLVAGNLYVVPANRHVVIRDGIVALGED
                     QADRPRPSIDLLLSTAARSYGEKLIAVILTGSGSDGAAGAVEVKEHGGTVVIQDPRTA
                     AHPSMPSALPPTVVDHVCHLADIAGMLRDILSGTTIEKHIDTGERGPLDEILAMVSRQ
                     GEVDFRQYKSATILRRIGRRMAINHLHTAEEYRDYLAANPQEATDLIRSLLIKVTEFF
                     RDGEAFDFLEQSVLPGLIAAGRERGRVLRFWCAGCATGEEGYSLALLLAHVLGRELPE
                     WSVRIFATDADEEAIAFARRGFYPPNVLKNLPEDYRTRFFEASDQGYRVIKPLRQMMI
                     FGQQDLSRGVPFPRIDLVTCRNVLIYFRPELQQAVLDLFAYSLHHTHGVLFLGKAETA
                     RPSRSTYEVVQKKWKIYRCVSGPLPMPARPQLESRRADRARDAAALAGLADGTTESRA
                     EEPETRRLHDALLRSLPFGLCIIDRSYRIQSINAAARRLLGVRDSVMDQDFLHTVRGL
                     PYGEVRAAIDRTFQERSVSVLAQVEMAEGIGEARFLALHIVPLEGGVVETAIVSVENV
                     TDLVQMQRRMQAVQSEQSHLAEELGAANRRLTDMNKDLQDANEELQAANEEMMLTQEE
                     LQATNEEFEATNEELQATNEELETGNEELQATNEELETTNDELQARTSELQEMARVLT
                     GERRRLAEVVEQSPFQVLLLRGPGLLVETMNPRLSMLFEGTPAAGRPFEEICVDRALE
                     AVRVGVRRAHADAKAWQSVPLRLAAGAERWLQFLAVPTHDAEGAVDGVALYVEDVTER
                     QRASR"
     gene            6212..6288
                     /locus_tag="VFM00_06215"
     tRNA            6212..6288
                     /locus_tag="VFM00_06215"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6246..6248,aa:Arg,seq:ccg)
     gene            6358..7524
                     /locus_tag="VFM00_06220"
     CDS             6358..7524
                     /locus_tag="VFM00_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004355668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HET7497875.1"
                     /translation="MNGRAADREPPLVAHVLYHLCMGGLENGLVNLINRIPPDRYRHA
                     VVCMAYYDDFRKRITRPDVEVFAMGVHERGVGRVTPDLVRLFRKIRPAMVHTRNPSGM
                     DALLPAALAGVPWRIHGEHGWETDDLQGRNRKNAWNRRLHAPLVNRFVALSRHQERYL
                     VERVGIPARRITQIYNGVDMERFRPREDAAPGAGGGAFMIGTVGRMQDVKDPLNLVEA
                     FLLLRRSRPDLAPRVRLAMVGDGSLRAAVESRLREGSAWEAASLPGAREDVAEQLRAI
                     DLFALPSKAEGISNTILEAMATGLPVVATRVGGNPELVEEGRTGLLVPPSDPAALAAA
                     LARYAEDPALARAHGAAGRERARAEFSLERMVGRYTALYDEGARRGRPTPAGVA"
     gene            7535..9037
                     /locus_tag="VFM00_06225"
     CDS             7535..9037
                     /locus_tag="VFM00_06225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7497876.1"
                     /translation="MTLHPSLAPGTRPAAATLALALLALFIVFAHLGEGHLANFDDCY
                     YAEKAKEMVGSGDWITPHYDGQVSLDNPPMFLWLMTGAFLLFGIRDVAAILPSAIAGV
                     LCVVLLHRLALRLGLSSFAAWCAGVVLLTTQYFLKYSRHAMFDVFLTLLFLLAIHAYL
                     RARDARPAFYALLGLLCGLGVLTKNVLGLFPLVVAALHLAWTGRARDLLRPPFWLAPI
                     AAAATVAPWYLAQLGAHRAQFVEEHFRWLLLERGFAASSGEPGWINYLGYARDLATYY
                     WPWLPVAVWGVVLAVRRSRGSVLGESDPAMPRDAAKLILLWILVVIGVMSVGREKKLW
                     YIMTAFPALALAAAIALDAWIREASARRRAVTWSVALLAALALALNFVPSRRGKERLP
                     GLYPIARVVSSAAAPGTRIVNYANGYWSIQNFMLYYSGHGLTKPTSDPDSARAALDGG
                     ALALVASRRAAEIAGADSTRYRAWVRSGEWTLMRAGPERPVTIPSNDPYR"
     gene            9049..9273
                     /locus_tag="VFM00_06230"
     CDS             9049..9273
                     /locus_tag="VFM00_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497877.1"
                     /translation="MSGFWTERRVARFITILASAAVVAADVYLFFMFRQHAGSFPPPW
                     DRAIPVGIAGILLFAVWRLRRQIRLFRMDR"
     gene            9600..10703
                     /locus_tag="VFM00_06235"
     CDS             9600..10703
                     /locus_tag="VFM00_06235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HET7497878.1"
                     /translation="MDDLAGNELFFTTLQEITSLINGGRAQETIFESVLSCALHMLHA
                     QAVFLVTVDGGRIRKFARRRGPDGQLQSLERYELPSHAGISRWVLLEGQPVHVPDVDA
                     DPRYHPSMDSLPGLQTATVLAAPLKVRDAVLGVLVAVNRLDATPFDPRHIRILSLLAN
                     QTAIAIENGKLYRHAEQLAITDDLTQVYNYRFLKMALRREVKRAARFGQTFSLLMIDV
                     DNLKHYNDQNGHLRGSEVLRSVAQILVREARSIDLVAKYGGDEFVVILPQTAREGAVV
                     LADRVKASVETTAFPLVPPGVITVSLGVATYPQNGITAGELLESADIALYAAKQSGKN
                     RVRSAGEPDARPGTRGKNPYDGGGAEPRGGESL"
     gene            10736..11404
                     /locus_tag="VFM00_06240"
     CDS             10736..11404
                     /locus_tag="VFM00_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497879.1"
                     /translation="MLVALHDPLLVAASFLVLFALVAVARAAGRHPSAAPAVRRTLHV
                     AVGLWTAFVTTRFHHLGWALVPPLSFLAINASGKTGRMVPALDRAGDAGSSRGLWTFP
                     LGVALTYALFWNDPERAPVVAGCLALALADPAAAWIGGRFGQRRLRPLRLRRTLEGSL
                     AFFLVAALAAGGVAWCLAPGVHAVRLAVGCAAAGAVVEALSPPGWDNAVIPLAVAAAF
                     HFLA"
     gene            11401..12135
                     /locus_tag="VFM00_06245"
     CDS             11401..12135
                     /locus_tag="VFM00_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HET7497880.1"
                     /translation="MTPFLWGLLFATAGAALVLATRQGTPGAAALGFAVSLALVLGFG
                     PGVMAPVALFVLGSGLLTRLGRARKERLRTAEKDRGRRGAAHVAAKLGVPALLGAAAA
                     LMPALASWLGGGAAASLAAAFADTSATEIGPLVAGQVFRVSGGRVTRAAHGAPGGVSA
                     AGLAGGALAALALAVLARGTGLGPAEGAPWAAAGAGFGAALVESAVAGTGVGRGLGPI
                     GRNLFLSALAAAAGVALAALVSPGGG"
BASE COUNT         1778 a         4349 c         4246 g         1895 t
ORIGIN      
        1 ccttcgggcg ccacgttcac ggccaacgcc tcgaacacgt cgggcacctt caactggacc
       61 ccgtcggcga cgcagtccgg cgtctacacc gtgacgttca cggcgcagaa cgcgctctcg
      121 ggctcggctt cgacggagat cacggtgaac aacgtcgacg cggctccggt ggtcacggct
      181 ccggcgacgc agtcgggccc cgagggcacc acgatcacct tcacggtgtc cgcgacggat
      241 cccgatggtg acgtgatcgt ttccctcacg ggctcaccga tcccggcggg agcgaccttc
      301 accgtcaccg gtgagggcac gccgacggcc accggcacct tctcctggcc caatgccacg
      361 ccggtgggga gctatccgat cacgtttacc gcctcgaatg tgctgacggg cacggcgacg
      421 acggtcgtca acgtcaccgg cgtgaaccac gaaccggtcg tcacggctcc ggccacggcc
      481 tccggcgatg agggaacgct gatcaccttc acggtctccg cgaccgacgc ggacggcgac
      541 cacgtgacgc tcacgatgct gggcggaccg tcgggcgcca cgttctcgga caacggcgac
      601 aacacgggca cgttcagctg gacgccgggc ttcaaccagg cgggcgtcta caccgtgaac
      661 ttccagggca gcgacggcaa cggcgccacg ggctcggcca gcacggtgat caccgtgaac
      721 gacgtgaacc gcgctccgac ggcggatgcc ggcggaccgt acacgggcgt gatcaacgtg
      781 gccgtctcgt tcgacggctc cggctcgtcc gatccggacg gcgacacgct gacgtatgcc
      841 tgggacttcg gtgacggcgg cacgggcagc ggcgtgaacc cgtcccacac ctacaccgcg
      901 ggcggcacct acaccgtgac gctcacggtc accgatccgg gcacgctctc gagcaccgcc
      961 acgaccaccg cgaccatctc gacgaccctg tcggcgaacg cgttcacggt cggcggcaac
     1021 aagacgacgt cgcttggcgc gggcaagccc tacacctgcg tccaggtgga agcggtggac
     1081 ggctcgttcc agaacaccga cgtcgacatc tccagcatca agatggtctc caatggcacc
     1141 ggttccgtct ccgagatctt cgccgatccg tcgaagagca gcgtcgacgg cgacaagaac
     1201 ggcaacggcg tcccggagat cgtggcgtgc ttcaagaagt ccgacctgcg gctcctgttc
     1261 agcaacctcg cggccggacg tcagacggtg aacgtcacga tcgaaggcag cctcgtgtcg
     1321 ggcgcgacct tcagcgctcc gctcacgatg atcgtcaaga gcaccggcgc ggttctggtg
     1381 gccagcgtgt cgccgaaccc gctcaatccg caggccaagc tcacgttcgc gacgagcaag
     1441 ccgggcgccg tccgggtcca gatgttcgac ccgcagggcc gtctggtgaa gacgatcgcc
     1501 gacgagcgcg cggccatggc cggctaccac gattacacga tcgacggacg ttcctcgacc
     1561 ggcgcgaagc tggcctcggg cgtctacttc gtgaagatcg tgtcggaggc ggatggcagc
     1621 acggtgacgc gagtcaccat catgaagtag gacgaccctc gaggggatcg tctgcaacac
     1681 ggacgggcgg ggcgcgcaag cgctccgccc gcttcgtttt ggggtacagg gaatcggctg
     1741 gtcgcccaag cgatccacgg tggactcggg ccggggcgcc gacgtaacgt acgcgcgctc
     1801 cgtcgcccat ccgaaaccgc ggccgttcga ccgaaccccc tggaggcaac ccatgaaggg
     1861 actccgccgc ctcaccgcat ggacgctgac tctcgcgctc ttcgttctcc cctcggtccg
     1921 agcccgcgcg ggcgacgacg tccggcgcgc ctacatcggc ggggccgcga ccctggagct
     1981 gcagggaagc cccgtcggct tcctccggag cgccagcggc ggcgatctgg agggcgacgt
     2041 ggtcacgagc gccgggccgg ccgtcaacaa gcacatcagc ggcatccact acaccgacct
     2101 cgttctggaa gtgggggcgg gcatggagcc ggcggtgtac gactggatcg ccgcggcgtg
     2161 ggacgcgaag gcgacccgag gctcgggagc ggtggcggtc gcggattaca acttcaacgt
     2221 gatcgcccgg aaggaattcc agcaaggcct gatcacggag acgtcactgc cggcgctgga
     2281 cgccgccagc aaggacccgg cgttcctgac cgtacgggtc gctcccgagc tggtccggat
     2341 caccaagggc ggcggaaccg tgccttcgac cctggcgacc aagcagcgac cgtggctggc
     2401 gaacagcttc cggctggaga tcccggggct ggactgcacg cgggtgcgcc gcatcgagcc
     2461 gctccggatc gggacccgga tcgcgcggga cgaggtgggg aacgtccgga cccccgtcaa
     2521 ccagcccacg gcgaccagcg tctccgatct cgtgatcacg atgcccgagg cgtacgcgga
     2581 cacgtggcgc gactgggtgc agcaattcct ggtgcagggg cagtacaacg acagccagga
     2641 gaagaacggc gatctcgtcc tcctcgggcc cgacatgcag agcgagatcg cccgggtcca
     2701 tctctacggc ctgggcattt cgtccctgaa gcaggccagg gccgagagcg ccgatgccat
     2761 ccggaacgtc accgccacgc tctactgcga gcgcgcggcg ctggagtgga agggaggagg
     2821 cggcgcggtg atgaacgtcc gcgtcttcgg ccgctagaac atcaggccga aggtcaccgg
     2881 gatgatccgc gtcgagttct tctcgaggaa gctcgtgtcg aagccctggt cggtgtagac
     2941 gttctcgatc cgggcctcca cgaagccgtg catgctggcc agcttgaact ggactccggc
     3001 gccgccgtcg atcccgaact tggtctcgtg cgcggaggtt tccgtgccgg tggaatccat
     3061 cttggtctcc acgcggaagg cgcccaggct ggcggtgacg tacggccgga tcggacccag
     3121 cgacatgccg atcgtgatcc cgcccattcc cgagagaatg ttgccgctgc cggtggtccc
     3181 gagcggcgcg tgttccaggt cgaacttctg gtagccgagc gcgccgcgga ggccgagggg
     3241 gaacaccggc ggcttcacct cgaggatgcc gcgaacgtgg aatccgttct tcaaggcgtc
     3301 cttcgcgtgg ctgacgggga ggctcacgcc gccgccgatc ccgacctgga ccaccttccc
     3361 ggcctcaccg gggagcgcca gcgcgggcga agcggcgaga agcgacagga cggcgagaac
     3421 gaggatcgtt cgcgacatgg aaacctcccg tgggttgcgc tgcggcatgg aactgaagta
     3481 cggatgcggc aggatactcc ggcggcggcc cgagggcaaa gggggcgagg ggcctatcgc
     3541 gacgcgcgct gtcgttccgt gacgtcttcc acgtagagcg cgacgccgtc cacggcgccc
     3601 tcggcgtcgt gggtgggaac cgccaggaac tgcagccagc gctccgcgcc ggccgcgagg
     3661 cgcagcggga cgctctgcca ggccttcgcg tcggcgtggg cgcgccgaac ccccacccga
     3721 acggcctcca gcgcgcgatc gacgcagatc tcctcgaagg gccgccccgc cgccggcgtg
     3781 ccctcgaaga gcatcgagag ccgcggattc atcgtctcga cgagaagtcc cggcccccgc
     3841 aggagaagga cctggaacgg agactgctcc acgacctcgg cgaggcggcg gcgctcgccc
     3901 gtcagcacgc gcgccatctc ctggagctcc gacgtgcgcg cctgcagctc gtcgttggtc
     3961 gtctccagct cctcgttcgt cgcctgcagc tcctcgttgc cggtctccag ctcctcgttc
     4021 gtcgcctgca gctcttcgtt ggtcgcctca aactcctcgt tggtcgcctg cagctcctcc
     4081 tgcgtcagca tcatctcttc gttggcggcc tgcagctcct cgttcgcgtc ctggagatcc
     4141 ttgttcatgt cggtcagccg ccgattggcg gcgcccagct cctcggccag gtgactctgc
     4201 tccgactgca ccgcctgcat gcggcgctgc atctggacga ggtcggtgac gttctccacg
     4261 gacacgatcg ccgtctcgac gacgcctccc tccagcggca cgatgtggag cgcgagaaaa
     4321 cgggcctcgc cgatcccttc ggccatctcc acctgcgcga gcaccgacac cgagcgctcc
     4381 tggaacgtcc ggtcgatggc cgcgcgcacc tcgccgtaag ggagtccccg aaccgtgtgc
     4441 aggaaatcct ggtccatgac cgaatcgcgg acccccagca gcctccgcgc ggcggcattg
     4501 atgctctgga tgcgatagga gcgatcgatg atgcacaggc cgaaggggag cgaccggagc
     4561 agggcgtcgt ggagccgccg ggtctccggt tcctcggcgc gcgactccgt cgtcccgtcg
     4621 gccagacccg cgagggccgc cgcgtcgcgt gccctgtcgg cgcggcgcga ttcgagctgc
     4681 gggcgcgccg gcatcggcag cggcccgctc acgcaccggt agatcttcca tttcttctgg
     4741 acgacctcgt acgtggagcg cgagggacgg gccgtctccg ccttgcccaa aaagaggacg
     4801 ccgtgcgtgt ggtggaggga gtaggcgaac aggtctagga ccgcctgctg cagctcgggc
     4861 cggaaataga tgagcacgtt ccggcaggtc acgaggtcga tccggggaaa gggaacgccc
     4921 cgactcaggt cctgctgtcc gaagatcatc atctggcgca gcggcttgat gacccgatag
     4981 ccctggtccg aggcctcgaa gaagcgggtc cggtagtcct ccggcaggtt cttgaggacg
     5041 ttcgggggat agaacccccg ccgggcgaac gcgatcgcct cttcgtccgc gtcggtggcg
     5101 aagatccgca cgctccattc cggcagctcc cggccgagga cgtgcgccag gaggagggcg
     5161 agcgagtatc cctcctcgcc ggtcgcgcat ccggcgcacc agaagcggag cacccggccc
     5221 cgctcgcgcc ccgccgcgat caggccgggc agcacgctct gctccaggaa atcgaaggcc
     5281 tcgccgtcgc ggaagaattc ggtgaccttg atcaggagcg atcggatcag atccgtggct
     5341 tcctggggat tcgccgcgag gtaatcgcgg tactcctcgg ccgtgtgcag atgattgatc
     5401 gccatccgcc ggccgatgcg gcgcaggatc gtcgccgatt tgtactgccg gaaatcgacc
     5461 tctccctgcc gggacaccat ggccaggatc tcgtccaagg ggccgcgttc cccggtgtcg
     5521 atgtgcttct cgatcgtcgt tccgctgaga atgtcgcgga gcatgccggc gatatccgcg
     5581 aggtggcaga cgtggtccac caccgtgggc gggagcgcgg agggcatcga gggatgggcc
     5641 gcggtgcgcg ggtcctggat cacgaccgtc ccgccgtgct ccttcacttc gacggcgccc
     5701 gccgcgccgt ccgagccgga tcccgtcagg atcaccgcga tcagcttctc gccgtaggaa
     5761 cgggcggccg tggacaggag tagatcgatc gagggccgcg gccggtcggc ctggtcctcg
     5821 ccgagcgcga cgatcccgtc gcggatgacc acgtggcggt tggccggcac cacgtacagg
     5881 ttcccggcca cgagcggcgt gtccccgttc acggtcgaca ccgtgagctc ggtccgccga
     5941 tccaggatgc cggcgagctg gctggggcgg gagggatcca ggtgctgcgc gaacacgacc
     6001 ggggcgggga agtccttgcc cagccccgag gccagcaccg agagcgcctc gatcccgccc
     6061 gccgaagagc cgacgacgac gagatgcccg tcgaccgatc cgtcaccagg gttcgtcatg
     6121 cgacctacgc tagtcgcggg accgggggga atcaagcgcg gaggcgctcc cgcttcccag
     6181 aatcggcgcg gacggttaga atggctcgct cgcgcccgta gctcagctgg atagagcgtc
     6241 tgcctccgga gcagaaggcc aggggttcga atcccttcgg gcgcaccatt atccctccgt
     6301 gattcgcggc gccgcgcgcc gcccgcagcc gacccgtcgg aggacgatcg acgccgcatg
     6361 aacggccgcg ccgcggatcg ggagcctcct ctcgtcgccc acgtcctcta ccacctctgc
     6421 atgggcgggc tggagaacgg gctcgtcaac ctgatcaacc ggatcccgcc cgaccgctac
     6481 cgccacgccg tcgtctgcat ggcgtactac gacgacttcc ggaagcgcat tacccgcccg
     6541 gacgtggagg tcttcgcgat gggggtgcac gagcgcggcg tgggccgcgt cacccccgac
     6601 ctggtccgcc tgttccggaa gattcgcccc gccatggtgc acacccggaa cccctccggg
     6661 atggacgcgc tccttccggc ggcgctcgcg ggcgtcccct ggcgcatcca cggcgagcac
     6721 ggctgggaga ccgacgatct ccagggccgc aatcggaaga acgcgtggaa ccggcgcctt
     6781 cacgcgccgc tcgtgaaccg gttcgtcgcc ctgtcgcggc accaggagcg ctatctcgtg
     6841 gagcgggtcg ggatcccggc gcggcggatc acgcagatct acaacggcgt cgacatggag
     6901 cggttccggc cgcgcgagga cgcggcgccg ggggccggcg gtggcgcctt catgatcggc
     6961 acggtcgggc gtatgcagga cgtaaaggat ccgctgaacc tggtcgaggc cttcctcctt
     7021 ctccggcgct cccgccccga cctggccccg cgcgtccggc tggcgatggt gggggacggg
     7081 tcgctccgcg cggccgtgga gtcgcgcctt cgcgagggaa gcgcgtggga ggccgcctcg
     7141 ctgccaggag cgcgcgagga cgtcgccgag cagctccgcg cgatcgatct gttcgctctt
     7201 ccctcgaaag cggaagggat ctcgaacacc atcctcgaag cgatggcgac ggggcttccc
     7261 gtggtcgcca cgcgcgtggg gggaaatccc gagctggtgg aggaggggag aacggggctg
     7321 ctcgttccgc cctccgatcc ggccgcgctg gccgcggcgc tcgcgcgcta cgcggaggat
     7381 cccgccctag cccgggctca cggggccgcc ggacgggagc gggcgcgcgc ggagttcagc
     7441 ctggagcgga tggtggggcg ctacacggct ctctacgacg agggagcacg gaggggccgt
     7501 ccgaccccgg cgggcgtcgc ttgacggcag tcccgtgacg ctgcacccgt ccctggctcc
     7561 cggcactcgc ccggccgcgg ccacgctggc gctcgctctc ctcgccctct tcatcgtctt
     7621 cgcccacctc ggggagggac atctcgccaa tttcgacgac tgctactacg cggagaaggc
     7681 gaaagagatg gtcgggagcg gcgattggat cacgccgcac tacgacgggc aggtctcgct
     7741 ggacaatccg ccgatgttcc tctggctcat gaccggagcg ttcctcctat tcggcatccg
     7801 cgacgtggct gcgatcctgc cctcggcgat cgccggcgtg ctctgcgtcg tgctgctgca
     7861 ccgcctggcg ctccgcctgg gcctgagctc cttcgcggcg tggtgcgccg gggtcgtcct
     7921 gctcacgacc cagtacttcc tcaagtactc gcggcacgcc atgttcgacg tgttcctgac
     7981 gctgctcttc ctgctcgcga tccatgccta tcttcgtgcg cgcgacgcgc gccccgcgtt
     8041 ctacgcgctg ctcggattgc tctgcggcct gggcgtcctc accaagaacg tgctcgggct
     8101 cttcccactg gtcgtggcgg cgctccatct cgcctggacg gggcgcgcgc gggaccttct
     8161 ccggccgccg ttctggctcg cgccgatcgc ggccgcggcg acggtcgcgc cctggtatct
     8221 cgctcagctg ggcgcccacc gcgcgcagtt cgtcgaggag cacttccggt ggctcctcct
     8281 ggagcggggt ttcgccgcct cgagcggcga gccgggatgg atcaactacc tcggctacgc
     8341 gcgcgacctc gccacctact actggccgtg gctgccggtc gcggtatggg gagtcgtcct
     8401 cgcggtccgc cgctcgcggg ggagcgtgtt gggggagagc gaccccgcga tgccgcgcga
     8461 cgccgccaag ctgatcctcc tctggatcct ggtcgtgatc ggcgtcatgt cggtgggacg
     8521 cgagaagaag ctctggtaca tcatgacggc cttcccggcg ctcgccctgg ccgcggccat
     8581 cgctctcgac gcctggatcc gcgaggcatc cgcgcgccgg cgcgcggtga cttggagcgt
     8641 ggcgctgctc gccgcgctcg ccctggccct caacttcgtg ccgtcgcggc gcgggaagga
     8701 gcgcctcccg ggcctctatc ccatcgcgcg cgtcgtctca tcggcggcgg cgcccggcac
     8761 gcgaatcgtg aactacgcga acggctactg gtcgatccag aacttcatgc tctactactc
     8821 cggccacggc ctcaccaagc cgacgtccga tcccgactcg gcgcgcgccg cgctggacgg
     8881 cggcgcgctg gcgctggtgg cgtcgcgccg tgccgcagag atcgcgggcg ccgattccac
     8941 gcgctatcgc gcgtgggtcc gctcagggga atggaccctg atgcgcgccg ggcccgagcg
     9001 accggtgacc attccctcga acgaccccta ccgctagact ccgccgccat gagtggattc
     9061 tggaccgagc gtcgcgtggc gcgcttcatc acgatcctcg cgagcgcggc ggtggtcgcc
     9121 gccgacgtct atctcttctt catgttccgg cagcacgcgg gctcgttccc cccaccctgg
     9181 gaccgcgcca ttcccgtcgg aatcgccggc atcctcctct tcgccgtgtg gcgccttcgc
     9241 cggcagatcc gcctcttccg catggatcgc taggcaagcg cttatggctt gtcgatttca
     9301 cgaatgaaga tgcccgggcc aagcgtgagt cggcttaggc acccgacgtc ggcaggtcga
     9361 aacgcaatcc gttgacgcga ggagaggcag ccgcggaggg cgcctcccgg aggcccgccg
     9421 aggaggctac ccaactcgag gagagagtcg ggccggaggg cggccgcccg aagggctgcc
     9481 tctcctcgcg caaacgcagt gcgcctgacc ccccgttgtc gcgatgcctc gcataaggca
     9541 cagagattgc atggcaccgc accgtgccac agcgccccaa caaaaccgtg catcccgcaa
     9601 tggacgacct tgcggggaac gaactgtttt ttaccaccct ccaggaaatt accagcctga
     9661 tcaacggcgg gagggcgcag gagaccattt tcgagtcggt cctctcctgc gcgctgcaca
     9721 tgcttcatgc gcaggcggtg ttcctcgtga cggtggacgg cggacggatc cggaagttcg
     9781 cccgccggcg cggcccggac ggacagctgc agtccttgga gcgctacgag ctgccgagcc
     9841 atgcgggcat cagccgctgg gtgctcctcg aggggcagcc ggtgcacgtg cccgacgtgg
     9901 acgccgatcc gcgctaccac cccagcatgg attccctgcc gggactgcag acggccaccg
     9961 tgctggcggc gccgctcaag gtgcgcgacg ccgtgctcgg cgtgctggtc gccgtcaacc
    10021 ggctcgacgc cacgcccttc gatccgcgcc acatccggat cctctcgctg ctcgccaacc
    10081 agacggcgat cgcgatcgag aacggaaagc tctaccggca cgcggagcag ctcgcgatca
    10141 ccgacgacct cacgcaggtc tacaactatc gcttcctcaa gatggcgctc cgccgcgagg
    10201 tgaagcgcgc ggcgcgcttc ggacagacct tctcgctcct catgatcgac gtggacaatc
    10261 tgaagcacta caacgaccag aatgggcacc tccgcggaag cgaggtgctc cgcagcgtgg
    10321 cgcagatcct ggtgcgggag gcgcgctcca tcgacctggt cgccaagtac ggcggcgacg
    10381 agttcgtcgt gatcctgccg cagacggcgc gcgagggggc ggtggtgctc gccgaccgcg
    10441 tgaaggcgtc ggtggagacg accgcgttcc cactggtgcc tcccggcgtc atcacggtca
    10501 gcctcggcgt cgcgacctat ccgcagaacg gcatcaccgc gggggagctg ctcgaatcgg
    10561 cagacatcgc tctctacgcg gcgaagcagt cgggaaagaa ccgcgtgcga agcgccggcg
    10621 agcccgacgc gcgtcccggg acccgcggca agaacccgta cgacgggggc ggcgcggagc
    10681 cgcgcggcgg cgaatcgctt tgacaccgcg ccgggcgcgc ggataaggtc ccctcgtgct
    10741 cgtggccctc cacgatcctc tcctcgtagc cgcctccttt ctcgtcctgt tcgctctcgt
    10801 ggccgtcgcg cgcgccgcgg gcaggcaccc ctcggccgca ccggccgtgc gccgcacgct
    10861 ccacgtcgcg gtcggcctct ggaccgcctt cgtcacgacg cggttccacc acctgggctg
    10921 ggcgctcgtt ccgccgctct ccttcctcgc gatcaatgcc tcggggaaga ccgggcgcat
    10981 ggtgccggcg ctggaccgcg ccggggatgc ggggtcttcg cgcggtctct ggaccttccc
    11041 gctcggcgtc gcgctcacgt acgcgctctt ctggaacgat ccggagcgcg cgccggtcgt
    11101 ggcgggctgc ctcgcgctcg cgctcgccga tcccgcggcc gcgtggatcg gcggacgatt
    11161 cgggcagcgg cgcctccgcc cgctccgcct gcggcgcaca ctcgagggat ccctcgcgtt
    11221 cttcctggtc gcggccctgg ccgccggcgg cgtcgcctgg tgcctggcgc cgggtgttca
    11281 tgccgtgcgc ctcgccgtgg ggtgcgccgc ggccggtgcg gtggtggaag cgctctcgcc
    11341 gccggggtgg gacaacgcgg tcatcccgct cgcggtcgcc gcggcgtttc acttcctcgc
    11401 gtgacgccgt ttctctgggg cctcctcttc gcgacggcgg gcgccgccct ggtgctcgcg
    11461 acccggcagg ggacgccggg cgccgcggcg ctcggcttcg ccgtctccct ggccctggtg
    11521 ttaggattcg gtcccggagt gatggctccg gtcgcgctct tcgtgctcgg ctcggggctc
    11581 ctcacgcgcc tcggacgcgc gcggaaggag cggctgcgca cggcggagaa ggaccgcggc
    11641 cggcgcggcg cggcgcatgt ggcggccaag ctgggggtgc cggcgctcct cggagccgcc
    11701 gccgccctca tgccggccct cgcgtcgtgg ctgggaggcg gcgcggccgc atcgctcgcg
    11761 gcggccttcg ccgacacctc ggcgaccgag atcgggccgc tggtcgcggg ccaggtgttt
    11821 cgcgtgagcg gcgggcgggt gacgcgcgcc gcgcatggcg cgcccggcgg cgtgagcgcc
    11881 gcggggctgg ccgggggagc gctcgccgcg ctcgccctcg ccgtgctggc gcgcgggacg
    11941 gggctgggac cggcggaggg cgcgccctgg gccgcggccg gcgcgggatt cggcgccgcg
    12001 cttgtggaga gcgccgtcgc cggcaccggg gtgggacgtg gcctcggccc gatcggacgg
    12061 aacctgttcc tgtcggcgct ggccgcggcc gccggcgttg cgctggccgc cctggtttcg
    12121 cctggaggag gttgacccgg atgtcgctcg acatcgcctg cgtcgtggcc gcccggccca
    12181 acttcatgaa gatggcgccg gtcgtcgagg cgctgcgcgc gcggccggga attcgcgcgt
    12241 ggctggtaca cacgggccag cattacga
//
LOCUS       DASSLZ010000160         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1447856, whole genome shotgun sequence.
ACCESSION   DASSLZ010000160 DASSLZ010000000
VERSION     DASSLZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447856"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFM00_06250"
     CDS             <1..555
                     /locus_tag="VFM00_06250"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7497881.1"
                     /translation="GVVSAPHLEEVDREHFLPSTRSVAFRRAAWERVHGFDERWDHNE
                     DTPFALSLKRAGCRFAFAPDAVVRWRPRGDLRSFWRQHRRFGYGDGESGVQGSFYGRI
                     AAKYGLALALLVAGAWFRPAWWALAAGAVLFALSQARRARGRLSPGAALTLVPALKVV
                     YDAAYLWGYARGVGSRGRSAMKPA"
     gene            585..938
                     /locus_tag="VFM00_06255"
     CDS             585..938
                     /locus_tag="VFM00_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497882.1"
                     /translation="MYEPRHKKPISRRQFALRVLHSAGLAFPLVLFSLGGGMAGYMLF
                     EKLSWVDAFLNASMLLGGMGPVATLKTEAGKIFAGIYALYAGLIFLIAAGLVLAPFFH
                     RVLHRFHWEQDQKPS"
     gene            935..1498
                     /locus_tag="VFM00_06260"
     CDS             935..1498
                     /locus_tag="VFM00_06260"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HET7497883.1"
                     /translation="MSDGATGAGASGGASERLVVPVAKGSLARFLAISLVGIAGFGYL
                     LFGRTAGPAPLGPALRALSAVGILFCAFSGLFAARRLFDPTPGLVLDAEGLIDNSNVI
                     GAGRVRWDEITEIRVTKSGPQRFLTVVVEDPRKFIDRGGSLRRKVYEANYRKAGTPVN
                     VTARTLAIPFEDLIAKTSEFYRRYGRQ"
     gene            complement(1521..2318)
                     /gene="cdaA"
                     /locus_tag="VFM00_06265"
     CDS             complement(1521..2318)
                     /gene="cdaA"
                     /locus_tag="VFM00_06265"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HET7497884.1"
                     /translation="MIDLKPQFLVDALDVLIVAFLFYRLFIMIKGTRASQMFVGLIII
                     VFASIVAQWFRLNALNWIIGSLKTVWVILFVILFQPELRALLTHIGQNRLVRALIRVE
                     EYGVVAETIKAVEEMAKERRGAIIVFERDMGLRDYVETGTKIDASVSAELLETIFTPH
                     SPLHDGAVVIRGDAIVAASCILPLSQTRGLSPLLGTRHRAALGLAEETDAAVIVVSEE
                     TGAISIAHKGELKWNLDEGQLRGELAAIFNPRPDETPEPEEVTTQEA"
     gene            complement(2315..3256)
                     /gene="folP"
                     /locus_tag="VFM00_06270"
     CDS             complement(2315..3256)
                     /gene="folP"
                     /locus_tag="VFM00_06270"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7497885.1"
                     /translation="MRRDPAVSETPPVSVFVHRAGSWNLHERARVAGILNLTPDSFYD
                     GGRWLDPARAVARAQAMAAEGADAIDVGAQSTRPGGGPPIGAEEEWARLRPVLPGVVR
                     LGLPVSVDTWSAEVARRALDEGVAIVNDVTGLTAQPELAAVVASSGAGLVLMHALGAP
                     DRMHEPVAYEDEALDVRRFLERQMKAATSAGIPEERIALDPGIGFSKRAPQSLRVLQG
                     LRSLTSLGRPLYIGVSRKSFLGRVTGRSIPIEDRLAAGLGATVAALALGARIVRTHDV
                     RETVDAIRTAEAILNPDARLAEDLPAGGAPVQAQARS"
     gene            complement(3261..>3522)
                     /locus_tag="VFM00_06275"
     CDS             complement(3261..>3522)
                     /locus_tag="VFM00_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HET7497886.1"
                     /translation="GAYNKARQLLADNTDKLHVIALALLEREVLDGDQLERLLRGEKL
                     EPLSRRGSGDGTAPVTAVEEEKPRAARSPAPGLRPSESPGPA"
BASE COUNT          484 a         1231 c         1254 g          553 t
ORIGIN      
        1 ggcgtggtct cggcaccgca tctcgaggaa gtggaccggg aacacttcct cccctccacc
       61 cgctcggtcg ccttccgccg cgccgcgtgg gagcgcgttc acggcttcga cgagcggtgg
      121 gaccacaacg aggacacgcc gttcgccctc tccctgaaac gggccggctg ccggttcgcg
      181 ttcgcccccg atgccgtggt gcggtggcgt ccgcggggcg atctccggag cttctggcgc
      241 cagcaccgcc gcttcggtta cggcgacggg gagtccggcg tccagggatc gttctacggt
      301 aggatcgccg ccaagtacgg actcgcgctc gccctcctcg tggccggcgc gtggttccgt
      361 cccgcgtggt gggcgctcgc cgccggcgcg gtcctgttcg cgctgagcca ggcgcgtcgc
      421 gccaggggcc gcctttcgcc gggagcagcc ctgaccctgg tgccggcgct caaggtggtc
      481 tacgacgcgg cctatctttg gggctacgcg cgcggggtcg gaagtcgcgg ccggagcgcc
      541 atgaagccgg cgtagcgcgc cggggactga gggggaggaa cgccgtgtac gagcctcgcc
      601 ataagaaacc gatctcccgc cggcagttcg ccctgcgggt cctgcacagt gcggggctcg
      661 cgtttccgct ggtgctcttt tcactgggcg gcgggatggc gggctacatg ctcttcgaga
      721 agctttcctg ggtggacgcg ttcctgaacg cgtcgatgct cctcgggggc atgggaccgg
      781 tggccacgtt gaagacggaa gcggggaaga tcttcgccgg gatctacgcc ctgtacgcgg
      841 gcctgatctt cctcatcgcg gccggcctcg tgctcgcccc gttcttccat cgcgtgctcc
      901 accgcttcca ctgggagcag gaccagaagc cgtcgtgagc gacggcgcca cgggtgcggg
      961 ggcgagcggc ggcgcgtccg agcggctggt cgtccccgtc gccaagggat cgctcgcccg
     1021 cttcctcgcc atctcgctcg tgggcatcgc cggcttcggc tacctgctct tcggccggac
     1081 ggcggggccg gccccgcttg gcccggcgct tcgcgcgctg tcggcggtcg gcatcctctt
     1141 ctgcgcgttc tcgggcctct tcgcggcgcg ccgcctcttc gatcccacgc cgggactggt
     1201 cctggacgcc gaggggctga tcgacaactc caacgtgatc ggcgccggcc gcgtgcggtg
     1261 ggacgaaatc accgagatcc gcgtcacgaa gtcggggccc cagcgcttcc tcacggtggt
     1321 ggtcgaagac ccgcgcaagt tcatcgaccg gggcggctcc ctgcgccgca aggtctacga
     1381 ggcgaactac cggaaagcgg ggacgccggt gaacgtcacg gcccgcacgc tcgccatccc
     1441 cttcgaggat ctcatcgcga agacgtcgga gttctaccgg cgatacggac ggcaataggg
     1501 gacgctcgcg gagggagccc tcacgcctcc tgcgtcgtga cttcctcggg ctcgggggtc
     1561 tcgtcgggac gggggttgaa gatcgccgcc agctcgcccc ggagctgccc ctcgtccagg
     1621 ttccacttga gctcgccctt gtgcgcgatc gagatcgcgc ccgtctcctc gctcaccacg
     1681 atcaccgcgg cgtcggtctc ctccgccagg ccgagggcgg cgcggtggcg cgtgcccaga
     1741 aggggcgaga gcccgcgcgt ctggctcaag ggaaggatgc agctggccgc gacgatggcg
     1801 tcgccccgga tcacgacggc tccgtcgtgc agcggcgaat ggggggtgaa gatggtttcc
     1861 aacagctcgg cgctcaccga ggcgtcgatc ttggtgccgg tctccacgta gtcgcgcagt
     1921 cccatgtccc gctcgaagac gatgatcgcc ccccgccgct ccttcgccat ctcctccacg
     1981 gccttgatcg tctcggccac cacgccgtac tcctcgacgc ggatgagcgc ccggaccagg
     2041 cggttctggc cgatgtgcgt gaggagcgcg cgcagctcgg gttggaagag gatcacgaag
     2101 aggatcaccc agaccgtctt cagactgccg atgatccagt tgagcgcgtt caggcggaac
     2161 cactgcgcca cgatcgaagc gaagacgatg atgatgagcc ccacgaacat ctgcgacgcg
     2221 cgcgtcccct tgatcatgat gaagaggcgg tagaagagga acgcgacgat caggacgtcc
     2281 agggcgtcga ccaggaactg gggcttgaga tcgatcatga tcgggcttgg gcctggaccg
     2341 gcgcgcctcc ggcgggaaga tcctccgcca ggcgcgcatc cggattgaga atcgcctcgg
     2401 cggtacggat cgcgtccacc gtctcccgca cgtcatgggt gcggacgatg cgcgccccca
     2461 gcgcgagcgc cgccaccgtg gcgcccagcc ccgcggccag ccgatcctcg atggggatgg
     2521 agcggccggt gacgcggccc agaaacgatt ttcgcgatac tccgatgtac aggggccgcc
     2581 cgagcgaggt caagctccga agtccctgga gcacgcgcag gctctgcggg gcgcgcttgg
     2641 agaagccgat cccagggtcc agcgcgatcc gctcctccgg gatacccgcc gacgtggcgg
     2701 ccttcatctg gcgctccagg aatcgccgca cgtccagggc ctcgtcctcg taggcgaccg
     2761 gctcgtgcat gcggtcgggg gcgcccaggg cgtgcatcag cacgagcccg gcgcccgaag
     2821 aggcgacgac ggcggccagc tccggctggg cggtgagacc ggttacgtcg ttcacgatcg
     2881 ccacaccctc gtccagagcc cggcgcgcca cctcggcgct ccaggtgtcc acggataccg
     2941 gaagacccag gcggaccacc cctgggagca ccgggcgaag ccgcgcccat tcttcttcgg
     3001 cgccgatggg aggaccgccg ccggggcggg tcgactgcgc gccgacgtcg atcgcgtcgg
     3061 caccctcggc ggccatggcc tgggcgcgcg cgacggcccg cgccgggtcg agccagcgtc
     3121 cgccgtcgta gaagctgtcg ggggtgaggt tgaggatgcc ggccacgcgg gcccgctcgt
     3181 ggagattcca cgagccggcc cggtggacga agacgcttac agggggcgtc tcggagacgg
     3241 ccgggtctcg ccgcacgtca tcacgccggt cccgggctct cgctggggcg cagaccgggc
     3301 gcgggactcc gggccgcgcg cggcttctcc tcttcgacgg cggtcacggg ggcggtgccg
     3361 tccccgctgc cgcggcggga gagcggctcc agcttctcgc cgcgcaggag cctctcgagt
     3421 tgatcgccat cgagcacctc gcgctccagg agcgcgagcg cgatgacgtg gagcttgtcg
     3481 gtgttgtcgg ccaggagctg gcgggccttg ttgtaggccc ct
//
LOCUS       DASSLZ010000161        10099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_435727, whole genome shotgun sequence.
ACCESSION   DASSLZ010000161 DASSLZ010000000
VERSION     DASSLZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10099
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435727"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..622
                     /locus_tag="VFM00_06280"
     CDS             98..622
                     /locus_tag="VFM00_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7497887.1"
                     /translation="MPARPPARFCIRCGTRMRPVREHGVMRPTCPACGFIAYRNPAPA
                     CGVLLQRGGQVLLARRGHEPRKDAWGIPAGFMEYGEHPETTAVREVLEETGLRIRLTG
                     LFGVYAGRDDPRTRAVLILYHGKIVGGRLQAGDDASELGWFALDRLPRPMAFRAHREA
                     LRDLRRANQRKTRA"
     gene            619..1764
                     /locus_tag="VFM00_06285"
     CDS             619..1764
                     /locus_tag="VFM00_06285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HET7497888.1"
                     /translation="MSVRILHLADLHLGAAFPTLGERAEERSRDVLSAFQRAVEFAAS
                     AEKPVDLVAIAGDLFDTHDPADGLVFEVESALERLTRAGLPVLIAPGTHDACGYRRSV
                     YRRMRLPEGAHLFLSPTFEPGPRHTVRGESVQTYGIAFDGAVCARPLSEFRPGELADY
                     HLGVAHGALQDSPTWKIRPQDMPITRSEIGACGLHYLALGHFHNFAEVREGGCVAVYP
                     GTLEGRKFGENGPRYLIVATLGREAVSIERTPWNARTVTEAVVDFQIAEIREARQLEE
                     RVAAFAGDREIVRVRLTGPAEFVFDAERLHARLKPRFFHLELDDRTYLVNARLLERYR
                     EEATVRGVFVRRMLERIEQAGTAEAKETATLALRVGLAEFQNPRHAP"
     gene            1754..3967
                     /locus_tag="VFM00_06290"
     CDS             1754..3967
                     /locus_tag="VFM00_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7497889.1"
                     /translation="MRLERVTVEGFGPLSNFDAALEPKRLNLIIGPNESGKSSFAGAI
                     VATLFGVSTLESEELARPWSGGAHAATIVFAAGTGRYRLRRNFQTQDVCVEQLDGEGD
                     EAKSVLFQAVANPRGKSPELQQYEELLRGWLGFTEARLFRESSFVYESALETRVSPEL
                     RHLVSGAVEADYQQIQEALLERLDTLTREHPYDPRQRKRNNRSIETREDRLEQLRARR
                     SRSENVLTELKSRTKEREGLESRITELRGELAGKERLLTDLETWVTLREEQRKLMKRV
                     PAVGQELVLARRARGQAEEIDRRISESLAYLANAPDEVEPDLLRLGMLRSQRSRHLKS
                     AETERTKLEGKKQGSPALAIVLALVGAAAAGGGVYAGLRNPLYAGAAGAVGALAGFAV
                     GRMFGQSAVKTRAVAEAQLKVAEENIRTLSQEIDQIEIRVNPYISGRTLEVVLADVKR
                     FRAANQERREHAAVMQSLPAPERLEAEAKELDEAVAALRAKEKAILRQSPFLAPLKED
                     PVKAAEAAERLKRETAGLRSKIEISQEGLDGLLRKAGGGEGDAENLETLDEMIGDEET
                     DLARERRQRDALLLALDVLRDSVIAYQQQHVGRLGGVAGSTLARLTAGRYRAVTLDSD
                     FTPMLATDGKERVPLEALSRGARDAFYFALRAALAKELAAREPVPLLLDDPIAHLDEE
                     RRGTMLAMLEELAREIQVVLLTHDRRVLNQVREAHVIALGAGVTPASSRKVEARK"
     gene            4053..5912
                     /locus_tag="VFM00_06295"
     CDS             4053..5912
                     /locus_tag="VFM00_06295"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET7497890.1"
                     /translation="MARPTPPPPTPDQKAARIRDQVLALDVKRVADWIQTLIRTVKAM
                     RMYLPNNPTLHKFQGDLEGRTWSVLKEIGDITLTVQQFDFLFENYSVYHNAAREDSLA
                     FRFYADGVREITIKEGLEPQELRGLLDVLKRSTDGPSGQDDVVTLLWERDFRHVEYIY
                     IPLDELIGDASPAPKGREEEAEASSEPGGGLPWPGSVAPEEEESDASATAGPDQAGAP
                     IAAERSDDWAPETSVDSAWDEAASVQFRMTDEELSTLEAGIREEEARPLGSEVLEIVA
                     AILESEEEPAGFLESAVAFQRFIELAIEEADIARANALLARLKTIAAKKAERHADFAG
                     IAEQVIREIGRPSFLAQAAPTLNAHPEIDPAVLTNFLIQLGSGAAPAVCDLLGQVNHV
                     KHRRALCEALATSCRDDVEILIGRLGDSRWYVIRNVVYVLGRIAHQGVERALDRALHH
                     EDVRVRKEAVRALGKIESPTSRAYLISAFRDADAGVRVQAATTLAAKRDERAAQSILQ
                     VIGAPEFPRRDLGERQAFFEALGRSGSDALVPQLSATLTKGTLFRAGSDEDRYHAALA
                     LAWLGTPAALAVLNRELSGKREPVRKAVEAALTVVRNAGMNTLGTAKSEEEGE"
     gene            5909..7384
                     /locus_tag="VFM00_06300"
     CDS             5909..7384
                     /locus_tag="VFM00_06300"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET7497891.1"
                     /translation="MSGTTKAPTPEAQRRGGDEAARVAVQGRGLAIHLCVLLKTARVH
                     DVGNVAFQGPLTNFMDTVEQMWTAEGDFKLQAIGDFLYLNQHRLRVDASSYPSYQYLI
                     DEFKARGLSGFVFTGKLTPPEVKKFVRLFLDVDVKAADPFDEFAEALSKLSVERLVPL
                     RTVVPNAGQIDSAEARDSRKAAKRTFYRAIESARTVMLSARDRRPIDLRKAKRAVQGI
                     VDLILEEEFSLFGLTAIKNHDEYTFQHCVNVSILSIALGQRLGLSKKLLGELGVAAIL
                     HDLGKATIPPWVLNKPGKLTAEEWKLMTDHPIQGVKMIAKMKGLNDLALRAMIVAFEH
                     HLNYDLSGYPQIGHEAKQTLFGRIVSIADCFDAMTAHRAYRRTPFTPYEALQQIVTKN
                     RDKFDPLLLKAFINTVGMYPAGTVVLLDTNEIAVVTAHNAHDIFRPKVRVVADKERKR
                     LEEGPVLDLSVRSEETGAFAVGIVSALNPEEYGVNVADALA"
     gene            7529..8920
                     /locus_tag="VFM00_06305"
     CDS             7529..8920
                     /locus_tag="VFM00_06305"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7497892.1"
                     /translation="MPLRRPRALIVDKDSAETHALIAALDADGFDAKWVKDGEQAFNV
                     LDAPGPDEAGAAAPGPEVVIAELRAQRVDGMRLLEVARRRNPEVCVILIADAGTIELA
                     TEAMRQGAYDFQTRPLNLAKMLAVIRRALSHQQLVGRVSDLAERLEERARVPSLTGNS
                     RAMQDLYARIAQVAGTRATVLIQGETGTGKELIAKALHQLSPRKDERFVKLHCAELSE
                     NIIESELFGHERGAFTGAEQQRRGRFELADQGTLFIDEISEVPMNIQTKLLRVLQDRQ
                     FERVGGEETVTVDTRVIAAANKPLDLLVARGQFREDLYYRLRVVLLEVPPLRERRDDI
                     PLLVEEFIHAFNKEHARKVTGVTRGVVDRMMQYDWPGNVRELKNTVEEMVLFTQGKRP
                     LDVSDLPIALRQQRTRTAAELNLAVGMSMAEIERAAIEATLRSVGYDKQKAAAALGIG
                     LRTLYRKVKEYGL"
     gene            8923..9237
                     /locus_tag="VFM00_06310"
     CDS             8923..9237
                     /locus_tag="VFM00_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497893.1"
                     /translation="MRGAWLAIALACSPVIARPVLAVAAYLHEFPHPVVAVQFHPRGD
                     RRFQVSDLENALHLGASAAELTVVISGFRAALERSIAPAALDTLCIVEAVRASAGVER
                     RP"
     gene            complement(9203..>10099)
                     /locus_tag="VFM00_06315"
     CDS             complement(9203..>10099)
                     /locus_tag="VFM00_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497894.1"
                     /translation="FFATQAPLVRPAYHPEFLDGIRYYVQSSGISSRFEGDSANYPVE
                     VAFYRWLQEHGRLVWSTRSEKASGPEIEVRELPGPLSTAAQRESLFAALMPHPNHTTR
                     LALWCADMSALFGKLNQPDRAEEWARRGLLVDSRNMNGQLQIGLAIALLGKGNAAGAE
                     AAARAGLALLPRSASAHFYRGLALRELGRKEEALPELKAALEATGDNRIRLNVAQILA
                     ELGRFEEAASQFEAVPQGVPERGLARRDLAVLYINKLHRPAEGIAAMQEAAALESDPR
                     EAALLRAEVARLTGGAPPPPRP"
BASE COUNT         1573 a         3533 c         3591 g         1402 t
ORIGIN      
        1 acgaagtaac aagcgtagga accccggagt gcgaacgcaa gttcgcgcgg cggcctcgcc
       61 gcgccgggtg gcgcgaggaa aagccgcggg gaaggtgatg cctgcgcgcc cgccggcgcg
      121 cttctgcatc cgctgcggca cgcggatgcg gccggtgcgc gagcacggcg taatgcgccc
      181 gacctgtccg gcgtgcggct tcatcgccta ccggaatccg gcgcccgcgt gcggggtgct
      241 tttgcagcgc ggcgggcagg tcctgctcgc gcggcgcggg cacgagccgc gcaaggacgc
      301 ttgggggatc ccggcgggct tcatggagta cggcgaacac ccggagacga cggccgttcg
      361 cgaggtgctc gaagagacgg ggctgcgcat ccgtctcacc ggcctcttcg gggtctacgc
      421 cggacgggac gatccgcgga cgcgggcggt gctgatcctc tatcacggga agatcgtggg
      481 aggacggctc caggcgggcg acgacgcgag cgagctgggc tggttcgcgc tggaccgtct
      541 cccgcggccg atggcgttcc gggcgcaccg cgaggcgctg cgcgacctgc gccgcgccaa
      601 ccagaggaag acgagagcat gagcgtacgg atcctccacc tggccgacct gcacctcggg
      661 gccgctttcc ccacgctggg ggagcgcgcc gaggagcgct cacgcgacgt gctctcggcg
      721 ttccagcgcg ccgtggagtt cgccgcctcc gccgagaagc cggtggacct ggtcgccatc
      781 gccggggacc tcttcgatac gcacgatccc gccgacggcc tcgtcttcga ggtggagtcg
      841 gcgctggagc ggctgacgcg cgcgggcctt ccggtactga tcgcgccggg cacccacgac
      901 gcgtgcggct accggcgctc ggtctatcgg cgcatgcgcc tccccgaggg ggcgcacctg
      961 ttcctctcgc ccacgttcga acccggcccg cggcatacgg tgcggggcga gtcggtgcag
     1021 acctacggca tcgcgttcga cggtgcggtc tgcgcccgcc cgctctccga gttccggccc
     1081 ggggagcttg ccgactacca cctcggcgtc gcgcacgggg cccttcaaga ttcgcccacc
     1141 tggaagatcc gtccgcagga catgccgatc acgcgcagcg agatcggcgc ctgcggcctc
     1201 cactacctgg cgctcgggca tttccacaat ttcgccgagg tgcgcgaggg aggctgcgtc
     1261 gcggtctacc cgggcaccct cgaggggcgc aagttcggcg agaacgggcc ccgctacctg
     1321 atcgtggcca ccctcggcag ggaggcggtc tcgatcgagc gcaccccgtg gaacgcgcgc
     1381 acggtgaccg aggcggtcgt ggacttccag atcgccgaga tccgcgaggc gcgccagctg
     1441 gaggagcggg tggcggcgtt cgcgggggac cgcgagatcg tgcgcgtccg cctcaccggc
     1501 cccgcggagt tcgtgttcga cgccgagcgt ctccacgcgc ggctcaagcc ccggttcttc
     1561 cacctcgagc tggacgaccg gacctacctg gtcaatgccc gccttctcga gcgctatcgc
     1621 gaggaggcca ccgtgcgcgg cgtgttcgtg cggcggatgc tcgagcggat cgagcaggcc
     1681 ggcaccgccg aggccaagga gaccgccacg ctcgcgctcc gggtcgggct cgccgagttc
     1741 cagaaccccc gccatgcgcc ttgaacgggt gacggtcgag ggcttcggcc ccctctcgaa
     1801 cttcgacgcg gccctcgagc cgaagcggct gaacctcatc atcggcccca acgagtcggg
     1861 caagtcctcg ttcgccggcg cgatcgtcgc gaccctgttc ggcgtctcca cgctggagtc
     1921 ggaggagctc gcgcgcccct ggagcggagg cgcgcacgcc gcgacgatcg tcttcgccgc
     1981 gggcaccggc cgctaccggc tgcgccggaa tttccagacg caggacgtct gcgtggagca
     2041 gctcgacggc gagggagacg aggcgaagag cgtcctcttc caggccgtcg cgaacccgcg
     2101 cggcaagagt cccgagctcc agcagtacga agagctgctc cgcggctggc tcggcttcac
     2161 cgaggcccgc ctcttccgcg agtcctcctt cgtctacgag agcgccttgg agacccgcgt
     2221 ctcccccgag ctccggcacc tggtctcggg ggccgtggag gccgactacc agcagatcca
     2281 ggaggcgttg ctggagcgcc tcgacaccct gacccgcgag catccgtacg acccgcgcca
     2341 gcgcaagcgc aacaaccgct cgatcgagac ccgcgaagac cgattggagc agctccgcgc
     2401 gcgacggtcc cgatcggaga acgtcctcac cgagctcaag tcgcgcacga aggagcgcga
     2461 ggggctggag tcccggatca ccgagctccg gggcgagctg gcgggcaagg agcggctgct
     2521 caccgacctc gagacctggg tgacgctccg cgaggagcag cggaagctga tgaagcgcgt
     2581 cccggccgtg gggcaggagc tggtcctggc gcgccgggcc cggggccagg cggaggagat
     2641 cgaccggagg atctcggagt cgctcgcgta cctggcgaac gcgccggacg aggtggaacc
     2701 cgatctcctg cggctgggca tgctccgctc gcagcggtcg cgtcacctga agagcgcgga
     2761 gaccgagcgg acgaagctgg aggggaagaa gcagggctct ccggcgctcg cgatcgtgct
     2821 ggcgctggtg ggcgccgcag cggccggagg gggcgtctac gccgggctcc ggaatccgct
     2881 ctacgcgggg gctgccggag cggtgggcgc gctggcgggg tttgccgtcg ggcggatgtt
     2941 cggccagagc gcggtcaaga cgcgcgccgt ggccgaggcg cagctcaagg tggccgagga
     3001 gaacatccgc acgctgagcc aggagatcga tcagatcgaa atccgcgtga atccctacat
     3061 ctcagggcgg accctcgagg tggtgctggc cgacgtgaag cgcttccgcg ccgcgaacca
     3121 ggagcgccgc gagcacgccg cggtgatgca gtccctgccc gcgccggagc gcctcgaggc
     3181 cgaggcgaag gagctggacg aggcggtggc ggccctgcgc gcgaaggaga aggcgatcct
     3241 gcgccagtcg ccattcctgg ctccgctcaa ggaggacccc gtcaaggccg ccgaggccgc
     3301 cgagcggctg aagcgcgaga ccgccgggct ccgctccaag atcgagatct cgcaggaggg
     3361 gctggacgga ctgctgcgga aggcgggcgg cggcgagggg gatgcggaga acctcgagac
     3421 gctggacgag atgatcggcg acgaggagac cgacctggcg cgggagcgcc ggcagcgcga
     3481 cgcgctcctc ctggcgctcg acgtgctgcg cgattcggtg atcgcctacc aacagcagca
     3541 cgtgggacgc ctgggcgggg tcgcgggctc cacgctggcc cgcctcacgg ccgggcgcta
     3601 tcgcgccgtg accctcgatt ccgacttcac gccgatgctc gcgaccgacg gcaaggagcg
     3661 ggtgccgctg gaggcgctga gccggggagc gcgggacgcc ttctacttcg cgctccgcgc
     3721 ggcgctcgcc aaggagctgg ccgcgcggga acccgtgccg ctcctcctgg acgatccgat
     3781 cgcgcacctg gacgaggagc gccgcgggac gatgctcgcc atgctggaag agctcgcgcg
     3841 cgagatccag gtggtgctcc tgacgcacga ccgccgcgtg ctgaaccagg tccgcgaggc
     3901 ccacgtgatc gccctgggcg ccggcgtgac cccagcgtcc tcccggaagg tcgaggcccg
     3961 caagtaagtc gcggctccgg accccgttcc gccgccgtac tcccccagaa tccgctcaag
     4021 atctggctcg atgcagccga agcacacacc gtatggcccg accgaccccg ccccctccga
     4081 ctccggatca gaaagcggcc cggatccgcg accaggtgct cgcgctcgac gtgaagcgcg
     4141 tggccgactg gatccagacg ctcatccgca cggtcaaggc catgcgcatg tacctgccga
     4201 acaatcccac gctgcacaaa ttccagggag acctggaagg gcgcacctgg tcggtgctga
     4261 aggagatcgg ggacatcacg ctcaccgtgc agcagttcga cttcctcttc gagaactact
     4321 ccgtctacca caacgccgcg cgcgaggact cgctcgcgtt ccgcttctac gccgacgggg
     4381 tgcgcgagat cacgatcaag gaggggctgg agccgcagga gctccgcggc ctgctcgacg
     4441 tgctcaagcg ctcgaccgac ggcccgtccg gccaggacga cgtcgtcaca ctcctctggg
     4501 agcgggactt ccgccacgtc gagtacatct acatccctct cgacgagctg atcggcgacg
     4561 cttcgccggc gccgaagggg cgcgaggaag aggcggaggc gagcagcgag cccggcgggg
     4621 gacttccctg gcccggcagc gtcgccccgg aagaggagga gtccgacgcc agcgcgacgg
     4681 ccggtccgga tcaggccggc gcgccgatcg cggccgagcg ctcggacgac tgggcgcccg
     4741 agacgtcggt ggactcggcg tgggacgagg ccgccagcgt gcagttccgt atgaccgatg
     4801 aggagctctc cacgctcgag gccgggatcc gggaggaaga ggcacgtccc ctcgggtcgg
     4861 aagtgctcga gatcgtggcg gccatcctcg aatcggagga ggagccggcc ggctttctgg
     4921 agtccgcggt ggcgttccag cgcttcatcg agctggccat cgaggaggcc gacatcgcgc
     4981 gcgccaacgc gctgcttgcc cgcctcaaga cgatcgccgc gaagaaggcg gagcgccacg
     5041 cggatttcgc cggcatcgcc gagcaggtga tccgcgagat cggccggccc agcttcctcg
     5101 cgcaggccgc ccccacgctg aacgcgcacc ccgagatcga tcccgcggtg ctcacgaact
     5161 tcctgatcca gctgggctcc ggcgcggcgc cggccgtttg cgacctgctc ggccaggtga
     5221 accacgtgaa gcaccggcgc gcgctctgcg aggcactggc cacctcgtgc cgggacgacg
     5281 tcgagatcct gatcggacgg ctcggcgact cacggtggta cgtcatccgg aacgtcgtgt
     5341 acgtgctggg ccgcatcgcg caccaggggg tggagcgcgc gctcgatcgg gccctgcatc
     5401 atgaggacgt ccgcgtgcgc aaggaagccg tgcgggcgct ggggaagatc gagagcccga
     5461 cgagccgcgc ctacctcatt tcggcgttcc gcgacgccga cgccggcgtg cgggtgcagg
     5521 cggccacgac cctcgcggcc aagcgcgacg agcgcgcggc gcagagcatt ctgcaggtga
     5581 tcggcgcgcc cgagtttccc cggcgcgacc tgggcgagcg gcaggccttc ttcgaggccc
     5641 tgggccgttc gggctccgac gcgctggtgc cgcagctctc ggccaccctc accaagggga
     5701 ccctgttccg cgccggaagc gacgaggatc gctaccacgc cgcgctcgcc ctggcctggc
     5761 tgggaacgcc ggcggcgctc gccgtgctga accgcgagct ctcgggcaag cgcgagccgg
     5821 tccggaaagc ggtggaggcg gcgctcaccg tcgtccggaa cgccggaatg aacacgctcg
     5881 gcacggctaa gtccgaggag gagggcgaat gagcgggacc accaaggcgc ccaccccgga
     5941 ggcgcagcgg cgcggcgggg acgaggcggc gcgcgtcgcc gtgcaggggc gcggcctcgc
     6001 catccacctc tgcgtgctcc tcaagaccgc gcgcgtccac gacgtcggga acgtggcctt
     6061 ccagggcccc ctcaccaact tcatggacac ggtggagcag atgtggacgg ccgaagggga
     6121 tttcaagctc caggcgatcg gcgacttcct ctatctgaac cagcaccggc tccgcgtcga
     6181 cgcatcgtcc taccccagct accagtacct gatcgacgag ttcaaggcgc gcggcctgtc
     6241 ggggttcgtc ttcaccggga agctcacccc tcccgaggtg aagaagttcg tccggctctt
     6301 cctggacgtg gacgtgaagg cggccgatcc cttcgacgaa ttcgccgaag ccctctccaa
     6361 gctctcggtc gagcggctcg tgccgcttcg cacggtggtg ccgaacgcgg ggcagatcga
     6421 cagcgccgag gcgcgcgaca gccggaaagc ggccaagcgg accttctacc gcgcgatcga
     6481 atcggcccgc accgtcatgc tgagcgcgcg ggacaggcgc cccatcgacc tccggaaagc
     6541 gaagcgcgcg gtgcagggga tcgtggacct gatcctggaa gaggagttct cgctcttcgg
     6601 cctcaccgcg atcaagaacc atgacgagta caccttccag cactgcgtca acgtctcgat
     6661 cctctcgatc gccctggggc agcggctcgg attgtcgaag aagctcttgg gcgaattggg
     6721 cgtcgcggcc atcctccacg acctgggcaa ggccacgatt ccgccctggg tgctcaacaa
     6781 gccgggcaag ctcacggccg aggagtggaa gctgatgacc gatcacccca tccagggggt
     6841 gaagatgatc gcgaagatga aaggactgaa cgacctggcg ctgcgcgcca tgatcgtggc
     6901 cttcgagcac cacctgaact acgacctgag cggctacccg cagatcgggc acgaggccaa
     6961 gcagaccctg ttcgggcgca tcgtctcgat cgccgactgc ttcgacgcga tgacggcgca
     7021 ccgggcctac cggcgcacgc cgttcacgcc ctacgaggcg ctccagcaga tcgtcacgaa
     7081 gaatcgagac aagttcgacc cgctgctcct caaggccttc atcaacacgg tcggcatgta
     7141 ccccgccggc accgtcgtgc tgctcgacac gaacgagatc gcggtcgtga ccgcgcacaa
     7201 cgcccacgac atcttccgcc ccaaggtgcg cgtcgtggcc gacaaggagc ggaagcgcct
     7261 ggaggagggt cccgtcctgg acctgagcgt ccggagcgaa gagaccggcg cgttcgcggt
     7321 cggcatcgtc tcggcgctga accccgagga gtacggcgtc aacgtcgccg acgcgctggc
     7381 ttaagcgcat cctgccgatc cgggtcctgc acaccgcccg ccgcgggtgg cgaccccgcc
     7441 ttccccatcg ctccttgacc cgccccgcat cccttcgtaa gctcttgcgc ctctgccctg
     7501 tagccaggat ctttggaggc tcccctccgt gccgctccgt cgtccccgcg cgctcatcgt
     7561 cgacaaggat tccgccgaga cccacgcgct gatcgccgcg ctggacgcgg acgggttcga
     7621 cgcgaagtgg gtgaaggacg gggagcaggc cttcaacgtc ctcgacgcgc ccggtcccga
     7681 cgaggccggc gccgccgccc ccgggcccga ggtcgtgatc gccgagctgc gcgcccagcg
     7741 ggtggacggg atgcgcctgc tcgaggtggc gcgccgccgc aacccggagg tctgcgtcat
     7801 cctgatcgcc gacgcgggca cgatcgagct cgcgaccgag gcgatgcgcc agggtgccta
     7861 cgatttccag acccgccccc tcaacctggc caagatgctg gctgtgatcc gccgcgcgct
     7921 gagccatcag cagctggtgg ggcgcgtctc cgatctggcc gagcggctgg aggagcgcgc
     7981 gcgcgtcccc agcctgaccg ggaattcgcg ggcgatgcag gacctctacg cgcgcatcgc
     8041 gcaggtggcg ggcacgcgcg ccacggtgct gatccagggg gagacgggaa ccgggaagga
     8101 gctgatcgcc aaggccctcc accagctctc cccgcggaag gacgagcgct tcgtgaagct
     8161 ccactgcgcc gagctctcgg agaacatcat cgagagcgag ctcttcgggc acgagcgggg
     8221 cgccttcacc ggcgctgagc agcaacggcg cgggcgcttc gagctggccg accaggggac
     8281 cctcttcatc gacgagatct ccgaggtccc gatgaacatc cagacgaagc tcctgcgcgt
     8341 gctgcaagac cggcagttcg agcgcgtggg gggcgaggag acggtgaccg tggacacacg
     8401 ggtgatcgcc gcggccaaca agcccctcga cctcctggtg gcgcgcggtc agttccggga
     8461 ggacctgtac taccggctcc gggtcgtcct cctggaggtg ccgccgctgc gggagcggcg
     8521 cgacgacatt cccctgctcg tcgaggagtt catccacgcg ttcaacaagg agcacgcgcg
     8581 caaggtgacc ggcgtgacgc gcggcgtggt ggaccggatg atgcagtacg actggccggg
     8641 gaacgtgcgc gaattgaaga acaccgtgga ggagatggtg ctcttcacgc aggggaagcg
     8701 tccgctggac gtgagcgatc tcccgatcgc gctccgccag cagcgcacga ggaccgcggc
     8761 cgagctgaac ctggccgtcg gcatgtcgat ggcggagatc gagcgggccg ccatcgaggc
     8821 gacgctgcgc agcgtcggct acgacaagca gaaggcggcg gccgcgctcg ggatcgggct
     8881 ccggacgctg taccggaagg tgaaggagta cggtttgtaa ccatgcgcgg ggcctggctg
     8941 gcgatcgctc tcgcctgttc ccccgtcatc gcgcggccgg tcctggccgt ggccgcgtac
     9001 cttcacgagt ttccacatcc cgtcgtcgcc gtgcagttcc atccgcgcgg agaccggagg
     9061 ttccaggtat ccgacctgga gaacgccctc catctcggcg cgagcgccgc cgagctcacg
     9121 gtcgtgatct cgggattccg cgcggcgctg gagcgctcga tcgcgccggc ggcgctggac
     9181 acgctctgca tcgtggaggc ggtcagggcc tcggcggggg tggagcgccg cccgtgaggc
     9241 gcgcgacctc ggcccggagc agcgccgcct cgcggggatc ggattccagg gcggccgctt
     9301 cctgcatcgc ggcgattccc tcggcggggc ggtggagctt gttgatgtag agcaccgcca
     9361 ggtcgcgccg cgcgaggccg cgctcgggca cgccctgcgg cacggcctcg aactgcgagg
     9421 ccgcttcctc gaaccgcccc agctcggcca ggatctgcgc cacgttgagt cggatccggt
     9481 tgtcgccggt cgcctccagc gccgccttca gctcgggcag cgcctcctcc ttccgcccca
     9541 gctcccggag cgcgagcccg cggtagaagt gcgccgacgc gctccgcggg aggagggcca
     9601 ggcccgcgcg cgcggccgcc tcggcccccg cggcgttccc cttgccgaga agcgctatgg
     9661 ccagcccgat ctggagctgg ccattcatgt tcctcgaatc gacgagcagc ccgcggcgcg
     9721 cccactcctc cgcgcggtcg ggctggttca gctttccgaa gagcgccgac atgtccgcgc
     9781 accagagcgc gagccgcgtc gtgtggttgg gatggggcat cagcgcggca aagaggctct
     9841 cgcgctgggc cgcggtgctg agcggtcccg gcagctcgcg cacctcgatc tccggtcccg
     9901 aggccttctc gcttcgcgtg ctccagacca ggcggccgtg ctcctggagc cagcggtaga
     9961 aggcgacctc gacggggtag ttcgcggaat cgccctcgaa gcggctggag atgccgctcg
    10021 actgcacgta gtagcggatg ccgtccagga actccgggtg gtacgccggt cgcacgaggg
    10081 gcgcctgcgt cgcgaagaa
//
LOCUS       DASSLZ010000162        12811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_171282, whole genome shotgun sequence.
ACCESSION   DASSLZ010000162 DASSLZ010000000
VERSION     DASSLZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171282"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VFM00_06320"
     CDS             <1..783
                     /locus_tag="VFM00_06320"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HET7497895.1"
                     /translation="PALLRTAVPPESFAGEFLSLPSRRGKHIVLECESRRAIVIHLMR
                     LGRLRLAPPTAGGGPSRRPAGSKGWSARIAFDDGSALLLIEHGTEKRARMWLTDDADA
                     LEELAAVGPDPTRGELSEERFLAALRASSRQLKTLLTDQRAIAGIGNGLSDEILYEAR
                     LSPLRLTGQVTDDEARRLYAAIGAVLERQTALLRESAAGELPQREPSEHYAVHDHAGS
                     KCPRCGGVIARISYADHETFYCPGCQTGGAPLKDRRLSKLLK"
     gene            919..1302
                     /locus_tag="VFM00_06325"
     CDS             919..1302
                     /locus_tag="VFM00_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497896.1"
                     /translation="MNPRRLIGSLVVLALLAGSTFVPLARAACALPKANTAACPSCVS
                     GPSSTGASVAADRSCCAAPSSLAERDPATVAPNGGFVETLAAAAILPAAHGAIVVSMQ
                     RDARTVAARPPGFSPPLLRTTILLI"
     gene            1433..2755
                     /locus_tag="VFM00_06330"
     CDS             1433..2755
                     /locus_tag="VFM00_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7497897.1"
                     /translation="MRRRAPLYLMFLMSLVLAGSVPSARAAETPPSESPASLDSIFGT
                     SSLDLAALEGAVLRRNPSLASMRSAWQAMEARADVAGAWDDPRVEGMLAPRSLGSSAV
                     DPGWSVEVTQMVPLFGQRAAMGKADRAQARSMREDYRAMRLEMLRMTRELYFQLFLTA
                     RSIDVNRELAGLLEQFRRVALQKYAAGTVGQQDALQADVEAAMLDHQLVATGRTRREI
                     QARLRALLHDETDRAFPDPPAELPHIQHELADTTFASAAATRPELRSRQAAIEARRAE
                     ITAASRMRLPELMLVARHDRTMEMPEWRTMVGAGITLPLWFGKNGAAVRAARADLERA
                     EAERLAVSDSIRAQVEIARARVHETSHELEIIETRVVPATERALASVRAGYESNRSDF
                     LALLTAERDLARARLDRYQSIAMVHMALADLERAIGLEPAPLREEESR"
     gene            2752..4317
                     /locus_tag="VFM00_06335"
     CDS             2752..4317
                     /locus_tag="VFM00_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7497898.1"
                     /translation="MRSERNRRLAARWTPVLAALLLAAVGGCSRKATTTDRSEGRAVG
                     DFRITAENHPARPVVGNNRLIVTLQDASGKPVVGATIDMSAVMEAMGTMPRMQSRGVV
                     TESRPGVYEVKYGLAMTGEWDLNLAIRASQGTQAVASWRLSTSTEDLQFVAGTPPPGA
                     APPPSASPTASAPGEIPGFGPVLLDTARRQSIGVGTQVVAPRPIRATIRAAGKVTYDE
                     TRRTEISLKFSGWVRRIQVDYTGRVVQKGEPLISVYSPELFSAQQEFLQALRPAPAGG
                     GSGMDMGPTGADASRTATGADPELAAAARQRLRLWDITPAQIDAIARSGKPLEAVPIL
                     APASGVVVEKNVVEGSSFMAGQVLYRIAPIHPVWVVASVYQYELPLVRSGMSAVIETP
                     FLPERTRTGRVSYINPYLDPSTRTGEVRVTVANPRGDLKPGMFVDVTLERDLGTRLAI
                     PAGAVLYEGNRSIVFVDRGPAGFAPREVALGAKAGDDVEVKAGLAPGDVIVTSGNFLI
                     AAESKLKAASVNP"
     gene            4328..7483
                     /locus_tag="VFM00_06340"
     CDS             4328..7483
                     /locus_tag="VFM00_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET7497899.1"
                     /translation="MLARLIAWCARNRGLTLALVLVAAAFGVRALQNTPLDAIPDLSD
                     TQVIISTEWMGRSPDLVEDQITYPIVTALRSAPGVQYVRGLSMIGDSFVYVVFKDGVD
                     VYWARSRVLEYLASLQGRLPEGVTPQLGPDATGVGWVFEYALVDTTGRNDLSQLRSFQ
                     DWYLRYWLQSVDGVAEVASVGGFVRQYQVDLDPRRLESYKISLKDVDMAIRRSNKDVG
                     GNVLEISEHEYFVRGRGYIRSTSDLEQVVVKVGEDGVPVTVGQVGEVHLGPEPRRGIA
                     ELNGRGEVVGGVVIMRQKQNALHVIQGVKRRLEEVRGAFPPGIQLVVTYDRSKLIGRA
                     VETLWHELLQEMLIVSLVIIVFLFHFRSALIPILTLPIAVVLSFILMQGQGLTANIMS
                     LGGIAVAIGAMVDASIIMVENVHKKLELWEAGDKRESRADAIIRGLQEVGRPVFFALM
                     VITVSFLPIFTLEATEGRLFKPLAFTKTYSMFFAALLAVTLTPALAAWFIRGKIRPEH
                     THPISRPLVQAYAPVVRFSVRHRRWIVLGAVLLMVSTLPIYLRLGSEFMPPLNEGTIL
                     HMPTAPPGMSATEAGRVLQYMDQKLKEIPEVETVFGKIGRARTATDPAPMSMVETVIT
                     LKPESEWRPGMTWERLISDMDRRVRVPGMPNIWWMPIQTRNEMLATGVRSAVGVKVYG
                     PDLATIERLAVQVERALQDVPGTRSAFAERIMGGLFLDFDIDRAEAARYGLTVEDVQD
                     VIETAVGGMPVSQTVEGRERYPINIRYARGFREDPEALGRILVPTMMGAQVPLAQLAK
                     LQFRTGPPMIQEEDGQLFGLVSVDVADRPLAQYVHDAKEAVAKKVVLPPRYRLDWSGQ
                     FKYYERAAARLALVVPITLLLVFGLLYFNTRSVTESSIVMLAVPFSLVGAVWILWILG
                     YHLSVAVWVGMIALAGLDAETGVVMLLYLKLAHKEREDRGQLRDRQDLTEAIVEGAAH
                     RIRPKMMTVCAILFGLLPILFGQGSGADVMKRIAAPMVGGVITSFILELIVYPAIYAG
                     WKGRHLPERSTASAPAEGDMK"
     gene            7480..7827
                     /locus_tag="VFM00_06345"
     CDS             7480..7827
                     /locus_tag="VFM00_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7497900.1"
                     /translation="MKTIIVAGLVVIGSITAVVGCQKSAPAGPQTVKVAVTDAGFVPA
                     QVDVRSGQPVVLLVTRTTDETCAKEIVIADEHIRRELPLNQEVRIAFTPEKKGEMKYV
                     CGMDMVSGTLRVE"
     gene            complement(7853..9031)
                     /locus_tag="VFM00_06350"
     CDS             complement(7853..9031)
                     /locus_tag="VFM00_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497901.1"
                     /translation="MHEHMEMTGMYGSYPMTREASGTSWQPDQARHQGLHRMSGAWML
                     MLHGSADLVADDQGGRRGDEKVFSSNMLMGMAQRDLGPGHLGLRAMVSAEPATLGKRG
                     YPLLLQTGETADGRTPLIDRQHPHDLFMELAATYSVTRGNRSLFLYGGLPGDPALGPP
                     AFMHRFSGAAIPQAPITHHWLDSSHITFGVLTAGAVVGGFKLEASAFRGREPDQNRFD
                     IESPKLDSHSFRVSLNPTPHWALQASRGRLKSPEQLEPEIDVDRTTASVLYAGDWKPG
                     HFESMLAWGRNRNRPGATLDAFTGEAALELGRRHTVFARVERVEKDELLPEGDPRAGR
                     VFDVGSVGAGYRFDFWSRGHFAAGLGLLATLSSVPEEIRADYGKDPASTMVFTRVTLR
                     "
     gene            complement(9276..10070)
                     /locus_tag="VFM00_06355"
     CDS             complement(9276..10070)
                     /locus_tag="VFM00_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7497902.1"
                     /translation="MKRAVILLLLLVVAVGAGAALSIFRPNLPAAERGRRLAEKTGCF
                     GCHGPGGIRGAMNPGRTDGSVPNFEGDVMMFAGDPEEIHEWIHNGVTAKRQKSETWRG
                     QRERGVLKMPAFKGRMTEARMHDLVAYVMAMAGMPAPEDSLASRGLERADSLGCLGCH
                     GAGGRLARNNPGSLKGYVPSWDGKDFSDLVRDSSEFRDWVEDGVSDRFESNPLAQFFL
                     RRAVLKMPAYKTHLQQGDVEALWAYVRWLRSEPEQTQIHEHEPVEN"
     gene            complement(10133..10507)
                     /locus_tag="VFM00_06360"
     CDS             complement(10133..10507)
                     /locus_tag="VFM00_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497903.1"
                     /translation="MKRFAIALAAALLLGSPYAAGAHEMSHGKTTTITGEVVDTGCYL
                     GHAARGASHTSCALKCVKGGMPMGVLTRQGALYLVTLNHDNPDPYNQLKDLAGKNVTV
                     TGTLVTRSGMKGIDVTSVKPAA"
     gene            11307..11696
                     /locus_tag="VFM00_06365"
     CDS             11307..11696
                     /locus_tag="VFM00_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7497904.1"
                     /translation="MVRTLNVREARIAAILLAVTIALAGGCSSKKEPKTVKAPPGNKD
                     VPIQVTEHGFEPARIEVTKGTNATLVFTRVTDRTCAKDIVIGGNGAHLDLPLNQPVRI
                     GLGTVNTNIPFACGMKMVTGEVVAVAQ"
     gene            complement(11749..12672)
                     /locus_tag="VFM00_06370"
     CDS             complement(11749..12672)
                     /locus_tag="VFM00_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HET7497905.1"
                     /translation="MNASSPSIPRILAAALFLTAALAPPAHASFHLNEFSRIMTGYNG
                     DATIQAVELRMLAGGENLVATGSIKTYDAAGNPLATLGTFAVNVPNGVLDRRILCATA
                     NFQSTFGIAADLTIAPGLPVGTGQVSFEEAGCFVNGIAYGAVTVPKNGVTSAAALPKD
                     LAYVLVRTVDDATLPSCPLAEDAAARMTLQSANAAAPVVFRNNAGASVSVSSAVTAVG
                     LLSRERSLGVAPNPVRSGARVTAPAGRRLTIHDARGRLVRVLAGARAESPYAAWWNGQ
                     DGRGAAVPSGVYFLRLQGPDGIVTRRFVVAR"
BASE COUNT         1998 a         4367 c         4446 g         2000 t
ORIGIN      
        1 ccggccctgc tccggaccgc cgtgcctccg gagtcgttcg ccggcgaatt cctgagcctc
       61 ccttcgcggc gcgggaagca catcgtgctc gagtgcgagt cccggcgcgc gatcgtcatc
      121 cacctgatgc gcctgggaag gctgaggctc gcgccgccga cggcgggagg gggcccgtca
      181 cgccgtcccg cgggcagcaa ggggtggtcg gcgcggatcg cgttcgacga cggatcggcg
      241 ctcctcctga tcgagcacgg caccgagaag cgcgcgcgca tgtggctgac cgacgacgcc
      301 gacgcgctcg aggaactcgc ggccgtcggc cccgacccca cgcgcggcga gctctccgag
      361 gagcggttcc tggcggcgct gcgcgcgtcg tcgcggcagc tgaagacgct cctcaccgac
      421 cagcgcgcga tcgccgggat cggaaacggc ctctcggacg agatcctcta cgaggcgcgg
      481 ctctcgccgc tccgcctgac gggccaggtc accgacgatg aggcgcggcg gctctacgcc
      541 gcgatcgggg cagtgctgga gcgccagacg gcgctgcttc gcgagtcggc cgccggcgag
      601 ctgccgcagc gggagcccag cgagcactac gccgtccacg atcacgcagg atcgaagtgc
      661 ccccgctgcg gaggagtgat cgcgcggatc tcctacgccg accacgagac gttctactgt
      721 cccgggtgcc agaccggcgg ggcgccgctc aaagaccggc ggttatccaa gctgttgaag
      781 tagaagggcg ttacccttac gtgaagggcg gtcacgagcc ggccgatacc aaggtatagt
      841 tgcaggaaga agctcccggc cgcatcgtat cccccatgag gcggctccga acggccgccc
      901 cggggtatga aggagcacat gaacccacgt cgcctcatcg gatcgctggt cgtgctggcc
      961 ctgctggcgg gatcgacgtt cgttcccctg gccagggcgg cgtgcgcgct tcccaaggcg
     1021 aacacggccg cctgcccgag ctgcgtgagc ggtccttctt cgacgggagc ttcggtcgcc
     1081 gccgatcgct cctgctgcgc ggctccgtcg tctctcgccg aacgcgatcc ggcgaccgtc
     1141 gcgcccaacg gcggcttcgt cgagacgctg gcggctgccg ccattctccc cgccgctcac
     1201 ggcgcgatcg tcgtatcgat gcagcgcgac gcgcggaccg tcgcggcccg tccgcctggt
     1261 ttttcgccgc cgctcctccg gaccaccatc ctcctgatct gagtcgcacc accttcccgg
     1321 gctgatccgg ggccggcgta cgccgcgccc ggacctgccg gccatgaccg ctcgtccgcg
     1381 cgcgtaacct cgcgcggata cccgtccacg agccaacgaa ggtggtgatc gcatgcgccg
     1441 tcgcgcgccg ctctatctca tgttcctgat gtcgttggtc ctcgccggct ccgtcccgtc
     1501 cgcgcgggcc gccgagacgc ccccgtcgga gtcgcccgcg tctctcgatt cgatcttcgg
     1561 gacctcgagc ctcgacctgg cggcactgga aggcgcggtt ctccgccgca acccctcgct
     1621 tgcctcgatg cgctccgcct ggcaggccat ggaggcgcgc gccgacgtcg ccggcgcctg
     1681 ggacgaccct cgcgtcgagg ggatgctcgc gccccgtagt ctcggctcct cggccgtcga
     1741 tccaggctgg tcggtcgagg tgacgcagat ggtgccgctg ttcggacagc gtgcggccat
     1801 ggggaaggcg gatcgcgctc aggcgcgctc gatgcgcgag gactaccgcg cgatgcggct
     1861 ggagatgctc cgcatgacgc gtgagctcta cttccagctc ttcctcacgg cgcggagcat
     1921 cgacgtcaat cgcgagctgg cggggctcct ggagcagttc cgcagggtgg cgctccagaa
     1981 atacgccgcg gggacggtcg ggcagcagga cgcgctacag gccgacgtcg aggcggcgat
     2041 gctcgaccac cagctcgtcg cgaccggccg cacgcggcgc gagatccagg cgcgcctccg
     2101 cgccctcctg cacgacgaga ccgaccgcgc gttccccgat ccgccggcgg agctcccgca
     2161 catccagcac gagctggccg acacgacgtt tgcctccgcc gccgcgacgc ggcccgagct
     2221 ccggtcgcgc caggccgcga tcgaagcgcg gcgcgcggag atcaccgcgg cgagccgcat
     2281 gcgccttccg gagctgatgc tcgtcgcgcg ccacgaccgg accatggaga tgcccgagtg
     2341 gcgcacgatg gttggggccg gcatcaccct cccgctctgg ttcggcaaga acggagccgc
     2401 cgtgcgcgcg gcgagggcgg acctcgagcg cgccgaggcg gagaggcttg cggtgtccga
     2461 ttcgatccgc gcccaggtcg agatcgcgag ggcgcgcgtc cacgagacga gccacgagct
     2521 ggagatcatc gagacgcggg tcgtccccgc caccgagcgc gcgctcgcct cggtacgcgc
     2581 cggctacgaa tccaaccggt ccgacttcct ggcgctcctc acggcggagc gggacctcgc
     2641 gcgggcccgg ctggaccgct atcagtcgat cgcgatggtc cacatggccc tcgccgatct
     2701 cgagcgcgcc atcggcctcg aacccgcgcc gctgcgcgag gaggaatctc gatgagatcc
     2761 gaacgaaatc gccgcctggc cgcgcgctgg acgccggttc tcgcggcgct gcttctcgcc
     2821 gcagtcgggg ggtgctcccg gaaggccacg accaccgacc ggagcgaagg tcgcgcggtg
     2881 ggggacttcc gcattacggc ggagaaccac cccgcgcggc ccgtggtggg gaacaaccgg
     2941 ctcatcgtca cgcttcaaga cgcctccggg aagccggttg tcggtgccac gatcgacatg
     3001 tcggccgtga tggaggcgat gggcacgatg ccgcgcatgc agagccgcgg cgtcgtcacc
     3061 gaatcgcgtc ccggcgtcta cgaagtcaag tacgggctcg cgatgacagg cgaatgggac
     3121 ctgaacctcg cgatccgcgc ctcccaggga acccaagcgg tcgcctcgtg gcgtctcagc
     3181 accagcaccg aggatctcca gttcgtcgcg ggaacaccgc cgcccggtgc cgcgccaccg
     3241 ccgagcgcgt ccccgacggc gtccgctccg ggggagattc ccggattcgg ccccgtgctg
     3301 ctcgacacgg cgcggcgcca gtcgatcggc gtcgggaccc aggtggtggc cccgcgtccc
     3361 atccgcgcca cgatccgcgc cgccgggaag gtgacgtacg acgagacgcg ccgcaccgag
     3421 atctcgctca agttctccgg gtgggtccgg cggatccagg tggactacac gggccgcgtg
     3481 gtccagaagg gggaaccgct catctcggtc tacagcccgg agctcttctc cgcgcagcag
     3541 gaattcctcc aagcgctccg tcccgcgccg gccggcggag ggtcggggat ggacatggga
     3601 cccacgggtg ccgatgcgag ccgcaccgcg accggcgccg atccggagtt ggccgcggcc
     3661 gcgcggcagc ggctccgcct ctgggacatc acgccggcgc agatcgacgc gatcgcgcgc
     3721 tcgggaaagc cgctcgaggc cgtgccgatc ctagcgcccg cgagcggcgt cgtcgtcgag
     3781 aagaacgtgg tggagggatc ctccttcatg gcgggtcagg tcctctaccg gatcgcgccg
     3841 atccaccccg tctgggtcgt ggcgagcgtc taccagtacg agctgccgct cgtgcggagc
     3901 gggatgagcg cggtcatcga aacgccgttc ctgccggagc ggacacgcac gggccgagtt
     3961 tcgtacatca acccctacct ggatccgagc acgcgtaccg gtgaggtgcg ggtcaccgtg
     4021 gccaacccga ggggagatct caagcccggc atgttcgtcg acgtcaccct cgagcgggac
     4081 ctggggacgc ggctcgcgat ccccgccggc gccgtgctgt acgaggggaa ccggagcatc
     4141 gtcttcgtgg accggggacc tgccgggttc gcgccgcgcg aagtggcgct gggggccaag
     4201 gcgggcgacg acgtcgaggt gaaggcgggc ctcgcacccg gagacgtgat cgtcacgtcg
     4261 ggtaacttcc tgatcgcggc ggagagcaag ctcaaggccg ccagcgtgaa cccgtaggag
     4321 ggcggccgtg ctcgcacgct tgatcgcctg gtgcgcccga aaccgcggcc tgacgctcgc
     4381 gctggtcctc gtcgccgccg ctttcggagt ccgcgccctt cagaacacgc cgctcgacgc
     4441 gattcccgac ctatcggaca cccaggtcat catctcgacc gagtggatgg ggcgtagccc
     4501 cgacctcgtc gaggaccaga tcacctatcc gatcgtgacc gcgctccgct ccgcgcccgg
     4561 cgtccagtac gtccgcgggc tcagcatgat cggcgactcc ttcgtctacg tcgtcttcaa
     4621 ggacggcgtg gacgtctact gggcgcggag ccgcgtcctg gagtatctcg cctcgctgca
     4681 ggggaggttg ccggagggag tcacgccgca gctcggtccc gacgcgaccg gcgtcggctg
     4741 ggtcttcgag tacgccctgg tcgacacgac cggccggaac gacctctccc agctccgctc
     4801 cttccaggac tggtacttgc gctactggct ccagagcgtc gacggcgtgg ccgaagtggc
     4861 ctcggtcggc ggcttcgtcc ggcagtacca ggtggacctc gatccgcgcc gcctcgagtc
     4921 ctacaagatc tcgctcaagg acgtcgacat ggcgatccgg cggtcgaaca aggacgtcgg
     4981 cggcaacgtg ctcgagatct ccgagcacga gtacttcgtg cgcgggcgcg gctacatccg
     5041 ctcgacctcc gacctcgagc aggtggtggt gaaggtcggc gaggatggcg ttcccgtgac
     5101 cgtggggcag gtgggcgagg tccacctcgg ccccgagccg cgccgcggca tcgcggagct
     5161 gaacggccgg ggcgaagtcg tcggcggcgt ggtcatcatg cgccagaagc agaacgccct
     5221 ccacgtcatc cagggagtga agcggcgcct ggaagaggtg cgtggagcgt ttcccccggg
     5281 gatccagctg gtcgtcacgt acgaccgttc gaagctgatc gggcgggcgg tcgaaaccct
     5341 ctggcacgag ctgctccagg aaatgctcat cgtcagcctc gtgatcatcg tgttcctgtt
     5401 ccatttccgg agcgccctga tcccgatcct cacgctgccg attgcggtcg tcctctcctt
     5461 catcctgatg caggggcagg gactgaccgc gaacatcatg tcccttggcg gcatcgccgt
     5521 ggcgatcgga gcgatggtgg acgcctcgat catcatggtc gagaacgtgc acaagaagct
     5581 ggagctctgg gaggccgggg acaaaaggga atcgcgagcg gacgcgatca ttcgcggcct
     5641 ccaggaagtg gggcgccccg tcttcttcgc cctgatggtg atcaccgtct ccttcctccc
     5701 gatcttcacg ctcgaggcga ccgagggacg cctcttcaag ccgctcgcct tcacgaagac
     5761 ctattccatg ttcttcgcag cgctcctggc ggtcacgctg acgccggcac tggcggcctg
     5821 gttcatccgc ggaaagatcc ggccggagca cacgcatccg atcagccgcc cgctcgttca
     5881 ggcgtacgcc cccgtggtcc gcttctcggt gcggcatcgc cgctggatcg ttctgggggc
     5941 ggtgctcctc atggtctcga ccctaccgat ctacctgcga cttggcagcg agttcatgcc
     6001 gccgttgaac gaggggacca tcctccacat gccgacggcg cccccgggaa tgtcggccac
     6061 cgaagcgggg cgcgtgctcc agtacatgga ccagaagctc aaagagattc ccgaggtcga
     6121 gaccgtcttc gggaagatcg gccgcgcgcg taccgcgacc gatccggcgc cgatgtccat
     6181 ggtcgaaacg gtgatcaccc tgaagccgga atcggagtgg cgccccggca tgacgtggga
     6241 gcggctcatc tcggacatgg accggcgcgt ccgagtcccc gggatgccga acatctggtg
     6301 gatgccgatc cagacccgga acgagatgct ggccaccggc gtccgaagcg ccgtgggggt
     6361 gaaggtctac ggacccgacc tcgccaccat cgagcgcctc gccgtgcagg tggaacgggc
     6421 gctgcaggac gttcccggca cccggagcgc ctttgccgag cggatcatgg gtggattgtt
     6481 cctcgacttc gacatcgacc gcgcggaagc ggcccgctac ggactcacgg tcgaagacgt
     6541 gcaggacgtg atcgagacgg ccgtgggcgg gatgccggtc tcgcagacgg tggaggggcg
     6601 cgaacgctac ccgatcaaca tccgctacgc gcgcggcttc cgggaggacc cggaggcgct
     6661 ggggcggatc ctcgtcccga ccatgatggg ggcgcaggtt ccgctggcgc agcttgccaa
     6721 gctccagttc cgcaccgggc cgcccatgat ccaggaggag gacgggcagc tgttcgggtt
     6781 ggtctcggtg gacgtcgccg accggccgct ggcccagtac gtgcacgacg cgaaggaagc
     6841 ggtcgcgaag aaggtcgtgc ttccgcctcg ataccggctc gactggtcag ggcagttcaa
     6901 gtactacgag cgcgccgcgg cccggctcgc gctcgtcgtc ccgatcacgc tgctgctcgt
     6961 gttcgggctc ctctacttca acacgcgttc cgtcaccgaa tcctcgatcg tgatgctcgc
     7021 ggttccgttc tcgctcgtgg gcgccgtctg gatcctctgg atcctgggct accacctgag
     7081 cgtggccgtc tgggtgggga tgatcgccct ggcaggtctc gatgccgaga cgggggtcgt
     7141 catgctgctc tatctcaagc tggcccacaa ggagcgggaa gaccgtggac agttgcggga
     7201 ccggcaggac ctgaccgaag cgatcgtcga gggagcggcg caccgcatcc ggccgaaaat
     7261 gatgactgta tgcgccattc tgttcggcct tttgcccatt ctcttcgggc agggaagcgg
     7321 cgccgacgtc atgaagcgga tcgccgcccc gatggtgggc ggcgtgatca cctcgttcat
     7381 cctggaactg atcgtctatc cggccatcta tgccggatgg aaaggtcgcc acctgccgga
     7441 gcgctcgacg gcttcggcac cagcagaagg agacatgaaa tgaaaacgat catcgtggca
     7501 ggactcgtgg tcatcggctc catcacagcc gtggtcgggt gtcagaagtc cgcgccggcc
     7561 ggtccgcaga cggtaaaagt cgccgtcacg gatgcgggat tcgtccccgc ccaggtcgac
     7621 gttcgaagcg ggcagccggt ggtcctcctc gtgacccgca cgaccgatga aacgtgcgcc
     7681 aaggagatcg tcatcgccga cgagcacatc cggcgcgagc tccccctgaa tcaggaagtc
     7741 cggatcgcgt ttactcccga gaagaagggc gagatgaagt acgtctgcgg gatggacatg
     7801 gtctccggca ccctgagggt ggagtgacgg agaagaagcc gtccccttgg cgctaccgga
     7861 gcgtcacgcg cgtgaacacc atcgtcgagg cggggtcctt cccgtagtcg gcgcggatct
     7921 cctcggggac gctcgagagc gtggcgagga gcccgaggcc ggcggcgaaa tgcccccggc
     7981 tccagaagtc gaaccggtac cccgcgccca ccgagccgac gtcgaagacc cgccccgcgc
     8041 gtgggtctcc ttcggggagg agctcgtcct tctcgacacg ctccacgcga gcgaagaccg
     8101 tgtgcctcct cccgagctcc agcgccgcct ctccggtaaa cgcgtcgagg gtggctccgg
     8161 gacgattccg gttccgcccc cacgccagca tcgattcgaa atgcccgggc ttccagtcgc
     8221 ccgcgtacag aaccgaagcg gtcgtgcgat cgacgtcgat ctccggctcc agctgctcgg
     8281 ggctcttcaa gcggccgcgg ctggcctgca gcgcccaatg cggcgtggga ttcagcgaga
     8341 cgcggaagga gtgcgagtcg agtttcgggg actcgatgtc gaaccggttc tggtcgggct
     8401 cgcgcccgcg gaacgccgag gcttccagct tgaagccgcc gaccacggcc ccggcggtca
     8461 gcaccccgaa cgtgatgtgc gacgagtcga gccaatgatg cgtgatcggg gcctggggaa
     8521 tcgccgcccc tgaaaagcga tgcatgaacg cgggcggccc cagcgcggga tcgcccggaa
     8581 gtccgccgta gaggaatagc gaccggttcc ctctcgtgac gctgtaggtg gccgcgagct
     8641 ccatgaagag atcgtgcggg tgctgccggt cgatgagcgg agtccttcca tccgccgtct
     8701 cgccggtctg caggaggagc gggtatccgc gcttcccgag cgtcgccggc tcggcggaga
     8761 ccatggcgcg caacccgagg tggcccgggc ccaggtcccg ctgcgccatt cccatgagca
     8821 tgttgctgct gaagaccttc tcgtcgcctc tccgcccccc ctggtcgtcg gcgaccaggt
     8881 ccgcggagcc atgcagcatc agcatccagg cgccgctcat ccggtggaga ccctgatgcc
     8941 gggcctgatc gggctgccag gaggtgccgg aagcctcgcg cgtcatgggg taggagccgt
     9001 acatgcccgt catctccatg tgctcatgca tgggcatctc gtggcccata ggcatgtcca
     9061 tcggcatgtc catgggctcg tgagccgggg gacgtttgga ccgcgccttc gccggggacg
     9121 gagtcttggg agggggcggc gcgtgatgat gggccgtcga atcggccatc gcggcggggt
     9181 ggtggtgcat ctgggcgcgg gagggggcgg cggatgccag ggcaacggcc gccatcgcgc
     9241 agcccgcgag aacgaatgag cgatgggtca tggcttcagt tctccacagg ttcgtgctcg
     9301 tggatctggg tctgctccgg ttcggagcgc agccaccgga cgtaggccca gagcgcttcc
     9361 acgtcgcctt gctggaggtg ggtcttgtag gcgggcatct tcaagacggc gcggcggaga
     9421 aagaattgcg ccaagggatt gctctcgaag cggtcgctga cgccgtcctc cacccagtcc
     9481 cggaattcgc tgctgtcgcg gacgagatcc gaaaaatcct tcccgtccca ggacgggacg
     9541 taccccttga gcgatccggg attgttccgc gcgaggcgcc cgcccgcccc gtgacacccc
     9601 aggcagccca ggctgtcggc ccgttcgagg ccacgggaag cgagcgaatc ctccggcgcc
     9661 ggcatccccg ccatggccat gacataggcg accaggtcgt gcatcctggc ttcggtcatt
     9721 cggcccttga acgcgggcat cttgagcacc ccgcgttccc tctggccacg ccaggtctcg
     9781 ctcttctggc gcttcgctgt gacgccgttg tggatccact catggatctc ctccgggtcg
     9841 ccggcgaaca tcatcacgtc tccctcgaag ttgggcaccg agccgtccgt gcggccgggg
     9901 ttcatggcgc cccggatccc tccgggtcca tgacatccga agcaccccgt cttctccgca
     9961 aggcgacggc cccgctcggc cgcgggcagg ttgggacgga agatcgagag ggcagcgccg
    10021 gctcccacgg cgacgaccag aagcagaagc aagatcacgg cacgtttcat cgcggctcct
    10081 tgcggcgcgc ggaatccgca cgcgcgccgc ctgggggaga tgtgcgccgc gcctacgcgg
    10141 cgggcttgac gctcgtgacg tcgatacctt tcatgccgct gcgggtgacc agggtgccgg
    10201 tgaccgttac gttctttccg gccaggtcct tgagctggtt gtagggatcg gggttgtcat
    10261 gattcaacgt gacgaggtag agcgctccct gacgcgtgag gacgcccatg ggcatgcccc
    10321 ccttgacgca cttgagcgcg cagctcgtgt gggacgcgcc gcgcgcggcg tggcccaggt
    10381 agcaccccgt gtccacgacc tctccggtga tggtggtcgt cttgccgtgg gacatttcgt
    10441 gggcgccggc ggcgtacggg ctgccgagca aaagcgccgc agcgagtgca atcgcgaatc
    10501 gtttcatgat ctctcctcaa ttgggttcgt ccaggcaccc cgcttcgcgg catggtgccc
    10561 gatacgctca tcgatgggga cgggaagata cgagcggcgc gtgggcggcc cgaacggcaa
    10621 ccgcctgccg cgaggagaaa ttctagagac gcagaatcga gtggagagcc gttcgtacgg
    10681 gggggccgtg ctccgtcgcg aagatggcga cggcaccgtc agcatcgttt cggggatcgc
    10741 acggggcgac ccaatcagcg gcgaagccga acgagtggat tggggttgat tgggcctcca
    10801 gggctgccgt ctcgatcgcg gccgcctgcg gcgggcacac cagatcccgg cacgggtcgt
    10861 ggctttcgtg gtgggccggc accgatccgt gctcatcacc gtcaggacag gccggcgatt
    10921 cgtgtgcggg tcctgacctc tcgggaggac agcagcacgc ggtcgccgac gccaccgctc
    10981 ccgcaaacgt gaggatgagg gcgagcgtcg cgagcaggtt ccgggccgag ccggcgaaga
    11041 tcgttgaagt cagagacgcc cccttcatga gggtgagtat gccgccgatg ccgaagggct
    11101 gcaatgcccc tcgtcccgag gacatcggga gggcactgaa ggtttagact ccaacgtttc
    11161 tcttcggggt tcaaggagcc gaatgccgcc gctcgccttg gaagcggcgt ctgtgccatc
    11221 ctgagagggc gtggtggagt gcaggccggg cgggagatcc gcccgatgac cgccggccac
    11281 ggccggagcg aaaggagtta gtcaggatgg ttcgaacgct gaacgtgcgg gaggcgcgaa
    11341 tcgccgcgat tctcctggcc gtgacgatcg cgcttgcggg cggatgctcg tccaagaagg
    11401 agccgaagac ggtgaaagcg ccgcccggga acaaggacgt gccgatccag gtgaccgagc
    11461 acggattcga gcccgcgcgg atcgaggtta cgaagggaac gaacgcgacc ctggtgttca
    11521 cgcgggtgac cgaccgcacc tgcgccaagg acatcgtcat cggcggcaac ggcgcgcacc
    11581 tcgacctgcc gttgaatcag ccggtgcgga tcggcctcgg caccgtgaac acgaacatcc
    11641 cgttcgcctg cgggatgaag atggtcaccg gagaggtggt cgccgtcgcg cagtagccgg
    11701 gacggcgccc gagtcgtaca ccggccgcgc tccgccgcgg ccggccgctc agcgggcgac
    11761 gacgaaccgg cgcgtcacga ttccgtccgg cccctggagg cgcaggaagt agacgcccga
    11821 gggcaccgcc gccccgcgcc cgtcctgtcc gttccaccac gccgcgtacg ggctctccgc
    11881 gcgggctccg gccagcaccc ggaccagccg cccgcgcgcg tcgtggatgg tgagccgccg
    11941 cccggcgggg gcggtcacgc gcgcccccga ccgcaccgga tttggagcga ccccgagcga
    12001 gcgctcgcgg gagagcaacc cgaccgcggt cacggcggag ctcacgctca ccgaggcgcc
    12061 cgcgttgttc cggaacacga ccggcgccgc cgcgttcgcc gactggaggg tcatccgcgc
    12121 cgccgcgtcc tcggcgaggg ggcaggaggg cagcgtcgcg tcgtcgacgg tccgcaccag
    12181 gacgtaggcc aggtccttgg gcagcgccgc ggcggacgtg actccgttct tgggaaccgt
    12241 caccgcgccg taggcgatcc cgttcacgaa gcagcccgcc tcctcgaacg acacctgtcc
    12301 cgtcccgacc ggcagcccgg gagcgatcgt gagatcggcg gcgattccga acgtggactg
    12361 gaagttggcg gtcgcgcaga gaatccggcg gtcgagcacg ccgttgggaa cgttcaccgc
    12421 gaaggtcccc agggtcgcga gcgggttgcc cgcggcgtcg taggtcttga tcgatcccgt
    12481 ggccacgaga ttttccccgc cggccagcat gcgcagctcg acggcctgga tcgtggcgtc
    12541 gccgttgtac ccggtcatga tccgggagaa ctcgttgaga tggaacgagg catgcgccgg
    12601 gggcgcgaga gcggccgtca ggaagagcgc cgcggcgagg atgcggggaa tcgagggcga
    12661 cgaagcgttc atcgggcagc ctccagtcca tggagcctgg cggccagcca acgtcggctc
    12721 gaccccatcg ttataatcct tctgggaacg gccctcaacc gaaaggagcg gtggagtgaa
    12781 atcttcgcgt catcggatcc gcgtttcgcc c
//
LOCUS       DASSLZ010000163         5427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_563327, whole genome shotgun sequence.
ACCESSION   DASSLZ010000163 DASSLZ010000000
VERSION     DASSLZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563327"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFM00_06375"
     CDS             complement(<1..717)
                     /locus_tag="VFM00_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HET7497906.1"
                     /translation="MSAVAEGARSAMDVRRLEPPDPERWDALVAEADGASIFHTSSWA
                     SVWTEEWKDARWEALVIEDDAGYAAGLGALVRRRGFWRSVDSMPFATYGGPIVRRGHP
                     DPSAARKALLEGFREWIRSRAVLRASLAWYEGRAAEFPEDLPPVASFTHVLALASDFE
                     ELASRFSPSTRRLIRQAEASGLSIRPAVTPEDLRAFYEIAVETVRRRGGQPKPFSLYE
                     RIAATLVPKGLARYHLVSRES"
     gene            complement(714..2915)
                     /locus_tag="VFM00_06380"
     CDS             complement(714..2915)
                     /locus_tag="VFM00_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HET7497907.1"
                     /translation="MTPRLRRLASIAAAAALFTPAPGVAATRLFIRNHVASNDVRGLA
                     AWQGSLVAATTGGLVTVAMPAGPLVKTIAAPGGLPSNRALCLVVSPSGDLWIGTADAG
                     IARRSPAGRWRRTLTTFDGLPSNDVQALLRSGDSVWVGTTGGVALFAETPANARVALR
                     RSDSRASTAGALVSDDVRALAMVRDTLWAGTGGGLSSFASGSWQDRTAVFGGAVDALL
                     AVGDTLWIGTDAGPRAYVNGVLAPVEPGYTGGSLALALDGGTLYSGSNLGVYRRTGGA
                     WSLTGPGLPFGSTRALGVAPDGALWAGAAAGLARFAAGAWTAYVTEGPAVESLQKAVV
                     RDHETWFTPGNQIAPGLGAGFVIRTDGTSWSVVSNTSTGGALQATSVFGILAAREGDL
                     WLGHCCASGVPRPRVERFDPDANAWVYPGPTNVLSFAQAPNGLVYGAGVELENGVYVF
                     DPSGAVVDSLTPLNTQGSARGSGLSSNNLRAIAFDPAGVAWIATADVGVDRWDGRGTA
                     AHADDFWDHFATGFPSPKTLAVVALDTTTAYVGTQAGIVVISGGRLDRTRMADVNDVI
                     GAVPVQDLARDPRRVVWIGTTAGLSRYDDAARTMERFTTADGLVDDDVRGLAWDEARG
                     ILWVATARGVSEVHPQAAGVPGLSSAAYAYPNPATGASGAVRIGGLTGAVTGEIRDLA
                     GNLVRNFRIDPVSSAAWDLRDASGAPAAPGVYLITLRDGSRVQTLRIAVTR"
     gene            complement(2912..4846)
                     /locus_tag="VFM00_06385"
     CDS             complement(2912..4846)
                     /locus_tag="VFM00_06385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7497908.1"
                     /translation="MALALVIVHYRTGDALARLLASLAATRPAPVSEIVVVNNSGEPI
                     DAVLAGSPWPVRVIAPGRNVGYARGVNAGIRAVGDRDVLVLNPDIQILPGAIEALVAA
                     AEAHPRAGILAPRLENLDGTLQLSARRFYNWKTLLLRRMPLGPFVAKSRVLRDHVMAD
                     WDHADLMRVDWVIGAAMLVRRRAMRDVGLMDERYFLYFEDVDWCQRMGRHGWEVLYVP
                     TARMVHEYARSSAQIRPRSLRAHAAGLLRFTEKWSALVYALSQNRGRLTQAAMLAADL
                     AAAAAACALAYGIRIALDAWFEKPVYPLPAYSGLFFFAIAVSVASLAWNGLYRRAAFR
                     DAIDRTFQVGRAVLQAGVLLMATTFLFQTPRYSRLLVLLLLPLELFCLLAVRSLLAGA
                     EAGAKRQGFAFRRVLLLGSGPEMEAARAALEGARASGFEPLHAEALAEAEAPEAAAAR
                     LRALVESERIQIVCIVPEAEELPYLLALASALRDSGAAIYWAGSLAHLVPGSGARALG
                     PVGAALLHAPSRGLSLRARKRASDVFLSVLVAPWRWGGLKRHLARNGGGLGPGEAWRR
                     VWSGERSWVGRTEYERDRWAGVPAWARVALESVRPGVVSPADENGRAPRLEAELAYLA
                     RFSLAEDLRIFLRATRGAGS"
     gene            complement(4854..>5427)
                     /locus_tag="VFM00_06390"
     CDS             complement(4854..>5427)
                     /locus_tag="VFM00_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HET7497909.1"
                     /translation="DEAAARDPRVHVMHRAGKQGLGSAYVAGFRYALDRDYEAVFEMD
                     ADFSHNPESLPVFLHELENADLVLGSRYLHGVTVVNWPIKRLILSYSANVYSRIITGM
                     RVKDLTGGFKCFRRRVLESIDWSRVRSDGYAFQIEITFKAWRKGFRIREIPIMFVDRK
                     AGESKMSRRIVHEAAWMVWRLRLLDLIGAL"
BASE COUNT          827 a         2000 c         1958 g          642 t
ORIGIN      
        1 cgattcccgc gagaccaggt ggtagcgggc gaggcccttg ggaacgagcg tcgcggcgat
       61 ccgctcgtag agcgagaacg gcttcggctg gccgccgcgc cggcgcaccg tctcgaccgc
      121 gatctcgtag aaagcgcgga gatcctccgg cgtgaccgcg ggccggatgg agaggccgct
      181 cgcctcggcc tgccggatga gccgccgggt cgagggggag aagcgcgacg cgagctcctc
      241 gaaatcggaa gccagcgcca gcacgtgcgt gaacgatgcg acaggaggga gatcctcggg
      301 gaattccgcc gccctgccct cgtaccacgc gagcgaggcg cggagcacgg cccggctccg
      361 gatccattcg cggaaaccct cgagcagcgc cttgcgcgcg gcggaaggat cggggtggcc
      421 gcggcggacg atcgggccgc cgtacgtggc gaagggcatg gaatccacgc tgcgccagaa
      481 gccgcggcgg cgcacgagag caccgaggcc cgcggcgtag cccgcgtcgt cctcgatcac
      541 gagcgcctcc catcgcgcgt ccttccactc ctccgtccag accgaagccc acgaggacgt
      601 gtggaagatg gacgcgccat cagcctcggc gaccagcgcg tcccatcgct ccgggtcggg
      661 aggctccagg cggcggacgt ccatggcgct ccgcgcgccc tccgcgaccg cgctcatcgc
      721 gtcaccgcga tccggagcgt ctggacgcgc gacccgtcgc gaagggtgat caggtagacg
      781 ccgggagccg ccggggctcc cgaggcgtcg cgcagatccc aggccgcgct cgagaccggg
      841 tcgatccgga agttccggac gaggttcccg gcgaggtcgc ggatctctcc ggtcacggca
      901 cccgtgagcc ccccgatccg gaccgcgccc gaggcgcccg tcgccgggtt ggggtaggcg
      961 taggcggccg acgagagacc gggcacgccc gcggcctgcg gatgcacttc cgagacgccg
     1021 cgcgccgtgg ccacccagag gatgccgcgc gcctcgtccc acgcgaggcc acgcacgtcg
     1081 tcgtccacca gcccgtccgc cgtcgtgaag cgctccatcg tgcgcgccgc gtcgtcgtag
     1141 cgcgaaagcc ccgccgtcgt gccgatccag accacccgcc gcgggtcgcg cgccaggtcc
     1201 tggaccggca cggcgccgat cacgtcgttc acgtcggcca tccgcgtccg gtccagacgt
     1261 ccgcccgaga tcacgacgat gcccgcctgc gttcccacgt acgcggtcgt cgtgtcgagc
     1321 gcgacgacgg cgagcgtctt ggggctcggg aatcccgtcg cgaaatggtc ccagaaatcg
     1381 tcggcgtgcg ccgcggtgcc gcggccgtcc cagcggtcca ccccgacgtc ggccgtcgcg
     1441 atccaggcga ccccggcggg gtcgaaggcg atcgcccgga ggttgttgct cgagaggccg
     1501 gacccgcgcg cggagccctg ggtgttgagc ggcgtgagcg agtcgacgac cgcgccggag
     1561 ggatcgaaga cgtagacgcc gttctccagc tccacccccg cgccgtagac gagtccgttt
     1621 ggcgcctgcg cgaacgacag gacgttggtc ggtcccgggt agacccaggc gttcgcgtcg
     1681 ggatcgaagc gctccacgcg cggacgcggc acgccggagg cgcagcagtg cccgagccag
     1741 agatccccct cgcgggctgc gagaatgccg aacaccgagg tcgcctggag cgcgcccccc
     1801 gtgctcgtgt tgctcacgac gctccacgag gtgccgtcgg tgcggatcac gaatcccgcg
     1861 ccaaggcccg gagcgatctg gttccccggc gtgaaccacg tctcgtgatc ccgcacgacg
     1921 gccttctgca gcgattcgac cgccgggcct tcggtcacgt aggcggtcca ggcgccggcg
     1981 gcgaagcgcg cgagtcccgc cgcggcgccg gcccagagcg cgccgtcggg cgcgacgccg
     2041 agcgctctcg tgctcccgaa gggaaggccc gggccggtga gcgaccaggc gccgccggtc
     2101 cggcgataga cgccgaggtt cgatcccgag tagagcgtgc cgccgtcgag ggcgagcgcg
     2161 agcgatcccc ccgtgtaacc cggctcgacc ggcgcgagca cgccgttcac gtaggcgcgg
     2221 ggccccgcgt cggtgccgat ccacagcgtg tcccccaccg cgagcagcgc gtcgaccgcg
     2281 ccgccgaaca ccgcggtccg gtcctgccac gagcccgagg cgaacgagga gagcccgccg
     2341 ccggtgcccg cccagagcgt gtcgcgaacc atcgcgagcg cccgcacgtc gtccgagacc
     2401 agcgcgcccg ccgtgctcgc gcgcgagtcg gagcggcgga gcgccacccg cgcgttcgcc
     2461 ggagtctcgg cgaagagcgc gacgcctccc gtggttccga cccacacgct gtcgccgctc
     2521 cggaggagcg cctgcacgtc gttggaaggg agcccgtcga acgtggtgag cgtgcggcgc
     2581 caccggcccg cgggagaccg ccgcgcgatc ccggcgtcgg ccgtgccgat ccagagatct
     2641 ccgctcgggc tgacgaccag gcagagcgcg cggttggacg gcagcccccc gggggcggcg
     2701 atcgtcttca cgagcggccc cgccggcatg gcgaccgtga ccagcccgcc cgtcgtggcc
     2761 gcgaccaggc tcccctgcca tgcggccagc ccgcgcacgt cgttcgaggc cacgtggttt
     2821 cggatgaaga gacgcgtggc ggccacgccc ggggccggcg tgaacagcgc cgccgccgcg
     2881 gcgatcgacg cgaggcgccg aaggcgcggg gtcacgaacc cgccccgcgc gtcgcgcgca
     2941 ggaagatgcg gagatcctcg gccaaggaga agcgcgcgag ataggcgagc tccgcctcga
     3001 gacgcggggc tcgcccgttc tcgtccgccg gcgacaccac gccggggcgc accgactcca
     3061 gggccacccg cgcccaggcg ggcacgcccg cccaacgatc ccgctcgtat tcggtgcgtc
     3121 ccacccagct tcgttccccc gaccagacgc gccgccaggc ctctcccggc cccaggcccc
     3181 cgccgtttcg ggccaggtgg cgcttcaggc cgccccagcg ccagggggcc acgagtaccg
     3241 agaggaagac gtcgctcgcc cgcttccgcg cgcgcagcga aagaccgcgg ctgggcgcgt
     3301 gcagcagcgc cgcgcccacg ggaccaagcg cgcgcgcgcc cgacccgggc accagatgcg
     3361 ccagcgagcc ggcccagtag atggcggcgc ccgaatcgcg cagcgccgag gccagcgcca
     3421 ggagatacgg cagctcctcc gcctcgggca cgatgcacac gatctggatc cgctcgctct
     3481 ccaccagcgc gcgcagccgt gccgccgcgg cttcgggcgc ctccgcctcc gccagcgctt
     3541 cggcgtgcag gggctcgaag cccgaggccc gggccccttc gagcgcggcg cgcgccgcct
     3601 ccatctccgg gcccgacccg agcaggagca cgcgccggaa ggcgaagccc tgccgcttcg
     3661 ctcccgcctc ggccccggcg aggagcgagc ggacggccag gaggcagaag agctcgagcg
     3721 gaaggagcag gagcacgagg agacgcgaat agcgcggcgt ctggaagagg aacgtcgtcg
     3781 ccatcagcag gacgcccgcc tggagcacgg cgcgccccac ctggaaggtc cggtcgatgg
     3841 cgtcgcggaa ggcggcgcgc ctgtagaggc cgttccacgc cagcgaggcc acgctcacgg
     3901 caatcgcgaa gaagaagagc cccgagtagg ccgggagcgg gtagaccggc ttctcgaacc
     3961 acgcgtcgag cgcgatccgg atgccatagg caagggcgca cgcggcggcg gcggcggcga
     4021 ggtcggccgc cagcatcgcc gcctgggtca gccgaccgcg gttctggctc agcgcgtaca
     4081 ccaaggcgct ccacttctcg gtgaagcgca agagacccgc cgcgtgcgcg cgcagcgagc
     4141 gcgggcggat ctgcgcgctg gaccgcgcgt actcgtgaac catccgtgcc gtggggacgt
     4201 acagcacctc ccagccatgg cgccccatgc gctggcacca gtccacgtct tcaaagtaga
     4261 ggaaatagcg ctcgtccatc aggccgacgt cgcgcatggc ccgccgccgc acgagcatcg
     4321 cggcgccgat cacccagtcg acccgcatga gatcggcatg gtcccaatcc gccatgacgt
     4381 ggtcgcgcag gacccggctc ttcgcgacga agggcccgag cggcatgcgg cggaggagga
     4441 gcgtcttcca attgtagaag cggcgcgccg agagttgaag cgtcccgtcc agattctcca
     4501 ggcggggcgc gaggatgccc gccctggggt gggcctccgc ggcggcgacc agcgcctcga
     4561 tggcgccggg caggatctgg atgtcgggat tcaggacgag gacgtcccgg tctcccacgg
     4621 cccggatccc ggcgttcacg ccgcgcgcgt acccgacgtt ccgcccggga gcgatcacgc
     4681 gcacgggcca gggcgagccc gcgagcacgg cgtcgatcgg ctcccccgag ttgttcacca
     4741 cgacgatctc ggagacgggc gcggggcgcg tggcggcgag cgaggcgagg aggcgcgcga
     4801 gggcgtcccc ggtccggtag tgcacgatga ccagggcgag cgccacggaa ggcttagagc
     4861 gcgccgatca ggtcgagcag ccggagtcgc cagaccatcc acgccgcctc gtggacgatg
     4921 cggcgcgaca tcttcgattc gcccgccttc cggtccacga acatgatcgg gatctcgcgg
     4981 atccggaatc ccttccgcca cgccttgaag gtgatctcga tctggaacgc gtagccgtcg
     5041 gagcgcacgc ggctccagtc gatcgattcg agcacgcgcc gccggaagca cttgaacccg
     5101 ccggtcagat ccttcacgcg cataccggtg atgatccggc tgtagacgtt ggcggagtag
     5161 gagaggatga gccgcttgat cggccagttc acgacggtga cgccgtgcag gtagcgcgat
     5221 cccaggacca ggtcggcgtt ctccagctcg tgcaggaaga cgggaaggct ctcggggttg
     5281 tgggagaagt cggcatccat ctcgaacacc gcctcgtagt cgcgatcgag cgcgtagcgg
     5341 aagcccgcga cgtaggccga gcccagcccc tgcttcccgg cgcggtgcat cacgtggacg
     5401 cgcgggtcgc gcgcggccgc ttcgtcg
//
LOCUS       DASSLZ010000164         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_689761, whole genome shotgun sequence.
ACCESSION   DASSLZ010000164 DASSLZ010000000
VERSION     DASSLZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689761"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..752)
                     /locus_tag="VFM00_06395"
     CDS             complement(147..752)
                     /locus_tag="VFM00_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7497910.1"
                     /translation="MRRILLFLLLAAAALSVAAPAQAQHRRYRDYDRPRNSSVEGQTI
                     LGIHLGLSSPSGGFDDIYNSGLGIGGSIAYGVSQSVLLSFELSHHQFDGDLPRDDASI
                     TPMTFNAAYVLPTGGSVIPWVGGGIGPYRHHERIEDGVAPGVDFEDTETAFGYNVGAG
                     IGGPIARRTLLGGGFRFHHVDGDHLLEAEFWTFQIGLGFML"
     gene            902..1819
                     /gene="hpnC"
                     /locus_tag="VFM00_06400"
     CDS             902..1819
                     /gene="hpnC"
                     /locus_tag="VFM00_06400"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HET7497911.1"
                     /translation="MLEPPPPLERIETDAPGGRPTDPTPLHLAYQACEAIARRHYENF
                     PVASWFLSHDRRQALAALYAFARIADDVADAERPSADRLERLDQIEADLHLALEGYPP
                     DPILLALADALDRHQLPAEPLFDLLEAFRTDARDAVFPTWDDLLGYCRGSANTIGRMV
                     LALYGVDDAPAQVESDALCTALQLTNFWQDLGTDLARGRLFVPLEDLARFGVARDRLA
                     DPSQAEAVTRLIEHECRATEELFDRGRGIVRRVPFGLSIQLRATVLGGTAVLRAVLRR
                     GQDVIHDRPRLGPLTRARIAAAACLGLPG"
     gene            1839..>2640
                     /gene="hpnD"
                     /locus_tag="VFM00_06405"
     CDS             1839..>2640
                     /gene="hpnD"
                     /locus_tag="VFM00_06405"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HET7497912.1"
                     /translation="MRRRSNFGVAFAILPRPQREAIRAVHAWSRALDDGVDEDSDTDR
                     ARARLERFRRELALLYEGVPEEPLTAALRPHVERFHIPRFYFEELINGVEMDLSHRRY
                     GTFPELHRYCYRVASIVGLICLRIFGEAEERGRRYAENLGLALQLTNILRDVGSDLSR
                     GRVYLPADELARFGVSEEALRMCRRDRAFLDLMRFQAERARSFFEAAEREVTALDRRR
                     LLAAEIMARVYRRLLERIEASGFDVLSREVRVSKLERVWIAASTALAVR"
BASE COUNT          371 a          970 c          898 g          401 t
ORIGIN      
        1 gacgcatcac ccgccgcggc ttcgcgcccg gcgcccaggg caagtcctgc gaggagaacg
       61 acgaagaaga gaaagggacg ggagcgcatg attcgacccc tccgacgtga tccggccagt
      121 cgagcctaca cccccgtccc gttctgctac agcatgaatc ccagcccgat ctggaacgtc
      181 cagaattccg cctcgagcag gtggtcgccg tccacgtgat ggaaccggaa gccgcctccc
      241 aggagcgttc tccgcgcgat cgggccgccg atcccggcgc ccacgttgta gccgaaggcg
      301 gtctcggtgt cctcgaagtc gacgccgggg gcgaccccgt cctcgatccg ctcgtgatgg
      361 cgataggggc cgattcctcc gccgacccac gggatcacgc tgcctccggt cggcaacacg
      421 taggccgcgt tgaacgtcat cggcgtgatg gaggcgtcgt cgcgaggcag gtcgccgtcg
      481 aactggtggt gggagagctc gaaggaaagg agcacgctct ggctcacgcc gtaggcgatg
      541 gagccgccga tgccgagccc tgagttgtag atgtcgtcga agccgcccga gggcgacgag
      601 aggccgaggt ggatgccgag aatcgtctgt ccttcgaccg agctgttacg gggacgatcg
      661 tagtcgcgat agcgccggtg ctgggcctgt gcgggagccg cgaccgagag agcggcggcg
      721 gccagaagta ggaacaagag gatgcgtctc atggaagtcc tccctggttg tggcgaaacc
      781 cctctcgatt tcgcgcgcgg aagcatagca cgtgagatcg gcgcccgctg ccgcgcgcct
      841 tgacgctccg tcgcgacgcg tgctctactg gcgcgagcgc atccccttcc aggaggcagc
      901 gatgctggaa cctccaccgc cgctcgagcg catcgaaacg gacgcgccgg gcgggcggcc
      961 aaccgatccc acgccgcttc atctcgcgta ccaggcctgc gaggccatcg cgcggcggca
     1021 ctacgagaat tttccggtcg cctcctggtt tttgtcgcac gaccggcgcc aggcgctggc
     1081 cgcgctctac gccttcgccc ggatcgccga cgacgtcgcc gacgccgagc gcccctcggc
     1141 ggaccgcctc gagcgcctgg accagatcga ggccgatctc cacctcgccc tcgaggggta
     1201 tccgcccgat ccgatcctcc tcgcgctggc cgacgcgctc gatcgccatc agctccccgc
     1261 cgagccgctg ttcgacctgc tcgaagcgtt ccggaccgac gcgcgcgacg cggtgttccc
     1321 gacctgggac gacctgctcg gctactgccg cggatcggcg aacacgatcg ggcggatggt
     1381 gctcgcgctc tatggcgtcg acgacgcgcc cgcccaggtc gagtcggacg cgctctgcac
     1441 cgcgctccag ctcaccaatt tctggcagga cctcgggacc gatctcgcgc gcggccgtct
     1501 cttcgtcccg ctcgaggatc tcgcgcgctt cggcgtggcc cgcgaccggc tcgccgatcc
     1561 ctcgcaggcg gaggcggtca cccggctgat cgagcacgag tgccgcgcca ccgaagagct
     1621 gttcgaccgc ggccgcggca tcgtgcggcg ggtgcccttc gggctctcga tccagctccg
     1681 cgccaccgtc cttgggggaa ccgccgtgct ccgcgccgtg ctccggcgcg ggcaggacgt
     1741 gatccacgac cgcccgcgcc tgggcccgct cacgcgcgcg cgcatcgccg cggccgcatg
     1801 cctcggactc cccggctgac gtggaggcct ccccggtgat gcgccgccgc agcaatttcg
     1861 gcgtcgcctt cgcgatcctg ccgcgcccgc agcgtgaggc gatccgcgcg gtgcacgcct
     1921 ggagccgcgc gctggacgac ggcgtggacg aggactcgga caccgaccgg gcgcgggcgc
     1981 gcctggagcg gttccgccgc gagctggcgc ttctctacga aggggtcccg gaggagccgc
     2041 tcacggccgc gctccgcccc cacgtcgagc ggttccacat cccccgcttc tatttcgagg
     2101 agctgatcaa cggcgtggag atggacctct cccaccgccg ctacggcacc ttccccgagc
     2161 tccaccgcta ctgctaccgg gtcgcttcca tcgtggggct catctgcctc cgcatcttcg
     2221 gcgaggcgga ggagcgcggg cggcgctatg ccgagaacct gggcctggcg ctccagctca
     2281 cgaacatcct ccgcgacgtg gggagcgacc tctcgcgcgg ccgcgtctat ctccccgccg
     2341 acgagctcgc gcgcttcggc gtgagcgagg aggcgctacg gatgtgccgg cgcgaccggg
     2401 cgttcctgga cctgatgcgc ttccaggcgg agcgcgcccg ttctttcttc gaggccgccg
     2461 agcgcgaggt gacggccctg gaccggcggc ggctcctcgc cgccgagatc atggcgcgcg
     2521 tctaccgccg cctgctggaa cggatcgagg cctccggctt cgacgtcctg tcgcgcgagg
     2581 tccgcgtctc gaagctggag cgcgtctgga tcgcggcgag caccgcgctc gccgtgcgca
//
LOCUS       DASSLZ010000165         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_312288, whole genome shotgun sequence.
ACCESSION   DASSLZ010000165 DASSLZ010000000
VERSION     DASSLZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312288"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..2493)
                     /locus_tag="VFM00_06410"
     CDS             complement(67..2493)
                     /locus_tag="VFM00_06410"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET7497913.1"
                     /translation="MKRPGLSTALAAAIASAIVLVFFYPLVFGKVFVSPDSVAPAGFS
                     KFAMEALKTRHVYALWNPFQFLGMPSFGSLAFVPYVYPLDPVFGFLNTALHFPDLTWL
                     LAHYLLIAVAMVLLARALGASSDAALIGATAFALTPNLVAVGAFGHGSQIMTAAYLPL
                     LFLLYDRFARRGSLLALAGFAFAAALQLLRSHVQIVFYTWLALGLYALFLAIAAARAR
                     RGGEAARALAGLAVGIGLGFGMSCFLYLPVHEYAKLSIRSAAEGSGAGFAYATSWSFH
                     PKEMLTFVIPSMFGFGGRTYWGTMPFTDYPNYMGIVPLALAVYGAARARGVMRGFWIA
                     LAAMALLISFGKHFAPLYQLLYERVPYFNKFRVPVMILVLLQFAVAVLAALGLDAALQ
                     GPVVPAAPARPAPGTKGRGTAAAKRKGSDGADFGARWMRWAIFAVAGGVGAVVLLQLL
                     GPSILAAASKAREYMTGDLARQALDMATLDALKSGILLAAAFFVLALHGRGRLSRAAA
                     ALLVVGITAVDLWGIDRKIMDPQIGSANEYAQNFTETPEIAFLKKDSTEFRVLPLRWD
                     DTRFSAFGIASIMGYHPAKPRLYQAFMDTVGIPNLRIVRLLNIKYVLTDGYYPDTTTD
                     LTLRFDGPVKVYEVKGTSPRAYMVHRVFPVKGESAALAMVRTAPVLGPGQYAVWMDPP
                     PYPKWPEPAAPDSVIRLRYDMNESDYMVSASTPGLFVQVDQWDPEWTATLDGAPVKVR
                     RVNYLMRGIEVPAGVHRIRLRYLPASLQAGIRISLASAGAAILLGIGGIFLRRRRARG
                     SAPAPPAEAA"
     gene            complement(2490..>3553)
                     /locus_tag="VFM00_06415"
     CDS             complement(2490..>3553)
                     /locus_tag="VFM00_06415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7497914.1"
                     /translation="TRFPDRARVLFVEPWTLGHRGAIRPADAAPGVRRVSFPFLPLHA
                     RSHGLRRAAYAVGSSALADGFFLLQRLGALGLRDGSRKRLALVQNFMAEPFLDALRPD
                     RVVYDMIDAPLHFAPVPPRLVPRWERLLRRADRVIVTSRALGSLATAAGARAATLIGN
                     GVEAARFDPDRVAPASLPGDPAAPVLGYVGSLHSWLDVPLVAALARACPEARVVLVGP
                     APPETAAALARAARENPNLHWTGPKPYDQVPGIVRAFRVGLIPFRRTPLTEAVNPVKL
                     YEYAAAGVPCVATRFTDEVDAWDGAARVAESPEGFIAEARALLASPPDRAGLLAFARR
                     HDWDAIAQRFASVCLEDAA"
BASE COUNT          602 a         1189 c         1345 g          417 t
ORIGIN      
        1 agatccgctc gagcagcgcc accacgttct ccttctcgtt gtacgtgggg acgatgatca
       61 gggccttcac gcggcctccg ccggcggggc cggcgcggac ccgcgcgcgc gccggcgcct
      121 caggaagatc ccgccgatcc cgagcaggat cgcggccccg gccgaagcga gcgagatccg
      181 gatccccgcc tggagcgacg ccgggaggta gcggagccgg atgcgatgca cgccggccgg
      241 cacctcgatc ccgcgcatca ggtagttcac gcgtcggacc ttcacgggcg ctccatcgag
      301 cgtcgccgtc cactcgggat cccactggtc cacctggacg aagaggccag gcgtggaggc
      361 gctgaccatg tagtcggact cgttcatgtc gtagcgcagg cggatcaccg aatcgggcgc
      421 ggcgggctcc ggccacttgg ggtacggcgg cggatccatc cagaccgcgt actggcccgg
      481 cccgagcacc ggcgccgtgc gcaccatggc gagcgcggcc gattcgccct tcaccgggaa
      541 gacgcgatgg accatgtagg cgcgcggcga cgtgcccttc acctcataga ccttcaccgg
      601 gccgtcgaag cgcagcgtca ggtcggtggt cgtgtcggga tagtagccgt cggtgagcac
      661 gtacttgatg ttcaggaggc gcacgatccg gaggttcggg attcccacgg tatccatgaa
      721 ggcctggtac aaccgcggct tcgcggggtg gtagcccatg atcgaggcga tgccgaacgc
      781 cgagaagcgg gtgtcgtccc accggagcgg gagcacccgg aactcggtcg agtccttctt
      841 caagaaagcg atctcgggcg tctcggtgaa gttctgcgcg tattcgttcg cgctcccgat
      901 ctggggatcc atgatcttcc ggtcgatccc ccagaggtcg acggccgtga tgccgacgac
      961 gagaagcgcc gccgcggcgc gggagagcct tccccgcccg tgcagggcca ggacgaagaa
     1021 ggcggccgcg agcaggattc cggatttgag cgcgtccagc gtggccatgt cgagggcctg
     1081 gcgggccaga tcccccgtca tgtactcgcg cgctttcgag gcggcggcga ggatcgaggg
     1141 gccgagcagc tggaggagca ccaccgcgcc gacgccgccg gcgacggcga agatggccca
     1201 tcgcatccag cgcgccccga aatcggcgcc gtcgcttccc ttccgtttgg cggccgccgt
     1261 cccccgcccc ttggtccccg gagcgggccg cgccggggcc gccggcacga cggggccctg
     1321 aagcgccgcg tccagcccga gcgcggccag cacggcgacc gcgaactgga gcagcaccag
     1381 gatcatcacg ggaacgcgga acttgttgaa gtaggggacg cgctcgtaga ggagctggta
     1441 gagcggcgcg aaatgcttcc cgaacgagat caggagcgcc atggcggcga gggcgatcca
     1501 gaagccgcgc atgacgccgc gcgcgcgcgc ggcgccgtag acggcgagcg cgagcggaac
     1561 gatccccatg tagttcggat agtcggtgaa cggcatcgtc ccccagtagg tgcgtccgcc
     1621 gaacccgaac atcgagggga tgacgaaggt gagcatctcc ttgggatgga acgaccacga
     1681 cgtggcgtac gcgaagccgg cgcccgaccc ctcggccgcg ctccggatcg agagcttggc
     1741 gtactcgtgg acgggcaggt agaggaagca gctcatcccg aaccccagcc cgatcccgac
     1801 cgccaggccg gccagcgcgc gcgcggcctc gccgccccgg cgcgcgcgcg ccgcggcgat
     1861 cgcgaggaag agcgcgtaga ggccgagggc aagccaggtg tagaagacga tctgcacgtg
     1921 cgagcgcagg agctggagcg ccgcggcgaa ggcgaatccc gcgagcgcga gcagcgatcc
     1981 gcgccgcgcg aaccggtcgt agagaaggaa caggagcggc aggtacgccg cggtcatgat
     2041 ctggcttccg tgcccgaacg cgcccacggc gaccaggttc ggcgtgaggg cgaacgccgt
     2101 agcgccgatc agcgccgcgt cggaggaggc gccgagcgcg cgcgccagga gcaccatcgc
     2161 gaccgcgatg agcaggtagt gcgcgagcag ccacgtgagg tcggggaaat ggagcgccgt
     2221 gttcaggaac ccgaagacgg gatcgagcgg atagacgtag gggacgaacg ccaggctccc
     2281 gaagctgggc atgcccagga actggaacgg attccagagc gcatagacgt ggcgcgtctt
     2341 cagcgcctcc atcgcgaact tcgagaagcc ggctggggcg accgagtcgg gcgagacgaa
     2401 gaccttcccg aagacgagcg gatagaagaa gacgaggacg atggctgagg cgatcgccgc
     2461 ggccagggcg gtggagagtc cgggtcgctt cacgccgcgt cctccaggca gaccgaggcg
     2521 aagcgttgcg cgatggcgtc ccagtcgtga cggcgcgcga aggcgaggag cccggcccgg
     2581 tcgggcggcg acgccaggag cgcccgcgct tcggcgatga agccctccgg ggattccgcg
     2641 acgcgggcgg cgccgtccca ggcgtccacc tcgtcggtga accgggtcgc cacgcaggga
     2701 acgccggcgg ccgcgtactc gtacagcttc accggattca ccgcctcggt gagcggcgtg
     2761 cgccggaagg ggatcagccc gacccggaac gcgcgcacga tgcccggcac ctggtcgtag
     2821 ggcttgggac cggtccagtg gaggttcggg ttctcgcgcg ccgcccgcgc gagcgccgcg
     2881 gcggtctcgg ggggcgcggg acccaccagg acgacgcgcg cctccgggca ggcgcgcgcg
     2941 agcgcggcca cgagcggcac gtcgagccag gaatggagcg agccgacata gcccagcacc
     3001 ggggcggcgg gatctccggg caaggacgcc ggcgcgaccc ggtcgggatc gaagcgcgcc
     3061 gcctcgacgc cgttgccgat gagcgtcgcc gcgcgcgctc cggccgcggt cgccagcgat
     3121 cccagcgcgc gcgacgtgac gatgacgcgg tcggcgcggc gcaggagccg ctcccagcgc
     3181 ggcacgagcc gcgggggtac cggagcgaag tggagcgggg cgtcgatcat gtcgtagacc
     3241 acccggtcgg ggcgaagggc gtccaggaac ggctcggcca tgaagttctg gaccagggcc
     3301 agccgcttcc gggagccgtc acgaagtccg agcgcgccga gccgctggag caggaagaat
     3361 ccatccgcga gcgcggacga tccgacggcg tacgcggcgc ggcgcaggcc gtgcgaccgg
     3421 gcgtgcagcg gcaggaacgg aaaggagacg cgtcgtacgc cgggcgcggc gtcggccgga
     3481 cggatggcgc cgcggtggcc gagcgtccag ggctcgacga acaggacgcg cgcccggtcc
     3541 ggaaagcgcg tcg
//
LOCUS       DASSLZ010000166         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_173461, whole genome shotgun sequence.
ACCESSION   DASSLZ010000166 DASSLZ010000000
VERSION     DASSLZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173461"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..610)
                     /locus_tag="VFM00_06420"
     CDS             complement(14..610)
                     /locus_tag="VFM00_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017509580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7497915.1"
                     /translation="MPVASGDLDRIVAFALELDRLKAVLRRTRPSRLDRQENSAEHSW
                     HVALLALSLAPYADPPVDGARVTELLLVHDVPEIDAGDTIVYGRTAGAVDEERRAAAR
                     VFGLLPDPQGSLLLARWEEFEARETAEARFAYAVDRLMPVLHNLHDGGRTWRENGIAL
                     NQVLAVNQAIGAACPALWEHVKRRVEDHFAHAQAKEIS"
     gene            complement(616..1146)
                     /locus_tag="VFM00_06425"
     CDS             complement(616..1146)
                     /locus_tag="VFM00_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497916.1"
                     /translation="MRRIAIAATALFLTVAAHPAFSAPPRVLEPLSFLLGAWEAPGPN
                     QSSGRTVFALSLQDQVMVRNNSADYPAADGKPATRHEDLMVIYPVGNVLRADYYDSEG
                     HAIRYVVLPAGAGRVLFLSDLIEKEPRYRLTYVLQPDGALAGAFEVAPSGQPDAFKSY
                     LTWQAHKAAPAAGAGQ"
     gene            1284..>2911
                     /locus_tag="VFM00_06430"
     CDS             1284..>2911
                     /locus_tag="VFM00_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7497917.1"
                     /translation="MSRAAATKPWGLYLAGLLALTFVVYWPSLQNGFVSWDDDFYVAM
                     NPVLARPSLAALITTPIAANWHPLTIASLLLDYRISGTSPATYHWINLLLHVANTALV
                     FVFVRALSRNRFWTTVVTALFFGIHPMHVESVAWIAERKDVLYGFFYLLALIFYVRRV
                     DGGGVGWIWAAFVSMALSVLAKPAAVVLPLSLLAIDWFRRRRFDARLWAEKVPFFALG
                     IAGGLVNLYVQKSLGALTVPHPWSLVDKVFFASYALVAYVAKLFVPVHLSAVYPYPAM
                     GAALPPRYVAAFLAVLVLLPLAIVVLRRNRPALFGIAFFFVNIVLVLQLITVGTAVLA
                     ERYTYLPYVGLFFALAWPLDDRGGSGFARGLRRALATLFVLLGVFSVAQTWSRCSVWH
                     DPETFWTDAIRKQPGEIVPAYVNRGTYYRRTGRLELARRDYTEALALNPQIPSVWYYQ
                     GTVFAALGLSDSAMVCFDRALEGDPRRRSALNDRGALRLRRGDAEGAVRDFSRLIELD
                     SLAWSAYSNRALAWSKLGENEKAVADGRKAVAVDP"
BASE COUNT          397 a         1055 c          935 g          524 t
ORIGIN      
        1 atgacgatac gtttcacgaa atctccttgg cctgggcgtg cgcgaagtga tcctcgacgc
       61 ggcgtttcac gtgctcccag agcgcgggac acgcggctcc gatcgcctgg ttgacggcaa
      121 gcacctggtt caaagcgatg ccgttctccc gccacgtgcg cccgccgtca tggagattgt
      181 ggagcaccgg catcagccgg tccacggcgt aggcgaagcg ggcctcggcg gtctcgcgcg
      241 cttcgaattc ctcccagcgc gccaggagaa gcgagccctg cggatcggga agaagcccaa
      301 acacgcgggc ggctgcccgg cgttcctcat ctaccgctcc cgcggtccgc ccgtacacga
      361 tggtgtcgcc ggcgtcgatc tccggcacgt cgtgcacgag caggagctcc gtcacgcgcg
      421 ccccgtccac gggcggatcg gcataagggg ccagcgacaa cgcgagcagc gcgacgtgcc
      481 agctgtgctc ggcgctgttc tcctggcgat ccagccgcga cggacgcgtc cgtcgcagaa
      541 ccgccttcag cctgtccagc tcgagcgcga acgcgacaat gcggtcgaga tcgccggacg
      601 ccacgggcat cgcggctact ggcccgcgcc cgctgccggc gccgccttgt gcgcctgcca
      661 cgtcaggtag ctcttgaacg catccggctg ccccgacggc gccacttcga acgcgccggc
      721 cagcgccccg tccggctgga gcacgtacgt gagccggtac cgtggctcct tctcgatcag
      781 atcgctcagg aacaggaccc ttcccgctcc ggccggcaac acgacgtagc ggatggcgtg
      841 cccctcgctg tcgtagtagt ccgcgcgcag cacgttcccg accgggtaga tgaccatcag
      901 gtcctcgtgg cgcgtcgccg gcttgccatc ggccgcggga tagtccgcgg agttgttgcg
      961 aaccatgacc tgatcctgaa ggctgagcgc gaagacggta cgtcccgagc tctgattcgg
     1021 ccccggcgcc tcccacgccc ccagaaggaa cgagagcggc tcgagcacgc gcggcggcgc
     1081 gctgaaagcc gggtgggcgg ccactgtcag gaacagcgct gtcgctgcga tggcgatgcg
     1141 tctcaaagcg gtctccttct tgggttcgat gggattgcgt ccctttgacc gcaagtctat
     1201 ggtgcggggg ccggacagtc catgccgtct ccttcccgtt ggaggctacg caccgccgcc
     1261 acgatgagcc gcacaccgcc acgatgagcc gtgccgccgc caccaagccg tggggcctct
     1321 atctggccgg cctcctcgcg ctcacgttcg tcgtctactg gccaagccta caaaacgggt
     1381 tcgtgagctg ggacgacgac ttctacgtcg ccatgaaccc ggtcctggcg cggcccagcc
     1441 tggccgcgct gatcacgacg ccgatcgcgg ccaactggca cccgctcacg atcgcgtcgc
     1501 tgctcctgga ttaccggatc tcgggcacgt cgcccgccac gtatcactgg atcaatctcc
     1561 tcctgcacgt ggcgaatacg gcgctggtct tcgtcttcgt gcgcgcccta tcgcggaacc
     1621 gcttctggac caccgtcgtc acggcgctct tcttcggcat ccatccgatg cacgtggagt
     1681 cggtcgcgtg gatcgcggag cggaaggacg tgctctacgg cttcttctac ctgctcgcgc
     1741 tgatcttcta cgtgcggcgc gtcgacggag gaggcgtggg ctggatctgg gcggccttcg
     1801 tctccatggc cctctcggtg ctcgccaagc cggccgccgt ggtgctcccg ctctcgcttc
     1861 ttgcgatcga ctggttccgg cggcggcgct tcgatgcgcg tctctgggcg gagaaggtcc
     1921 ccttcttcgc cctcgggatc gcgggcggcc tcgtgaacct gtacgtgcag aagtccctcg
     1981 gcgcgctgac cgtccctcac ccgtggagcc ttgtcgacaa ggtcttcttc gcctcgtacg
     2041 cgctcgtggc gtacgtcgcg aagctcttcg tgcccgtgca tctctcggcc gtttatccct
     2101 atccggccat gggcgcagcg ctcccgccgc gctacgtcgc cgcgttcctg gccgtcctgg
     2161 tcctcctgcc gctcgcgatc gtcgtcctcc gcagaaaccg ccccgccctg ttcggaatcg
     2221 ccttcttctt cgtcaacatc gtcctcgtgc tccagctgat caccgtcggc accgcggtgc
     2281 tggccgagcg atacacgtat ctgccgtacg tcggcctctt cttcgcgctc gcctggccgc
     2341 tcgacgatcg cggcggctcc ggcttcgcgc ggggcctccg ccgcgccctc gccaccctgt
     2401 tcgtgctgct tggagtcttc tccgtcgcgc agacctggag ccgctgctcg gtctggcacg
     2461 atccggagac gttctggacg gacgcgatcc ggaagcagcc cggcgagatc gtgccggcct
     2521 acgtgaaccg cggcacctac taccgtcgga ccggccgcct ggagctggcg cggcgcgact
     2581 acaccgaggc gctggcgctg aatccgcaga ttccgagcgt ctggtactac cagggaaccg
     2641 tgttcgcggc gctcgggctc tccgactccg cgatggtctg cttcgatcgc gcgctggaag
     2701 gcgatccgcg gcggcgctcc gcgctcaacg accgcggggc gcttcgcctg cgccggggcg
     2761 atgccgaggg cgccgtccgg gacttctcgc gcctgatcga gctcgattcc ctcgcctgga
     2821 gcgcctacag caatcgcgcc ctcgcctggt ccaagctcgg ggagaacgag aaggcggtgg
     2881 ccgatggacg caaggcggtc gcggtcgatc c
//
LOCUS       DASSLZ010000167        14658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_312559, whole genome shotgun sequence.
ACCESSION   DASSLZ010000167 DASSLZ010000000
VERSION     DASSLZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14658
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312559"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /locus_tag="VFM00_06435"
     CDS             complement(<1..1620)
                     /locus_tag="VFM00_06435"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HET7497918.1"
                     /translation="MTTETVAEAPARPVEAAPPPQTPEQIERHWFENVYAGDRMRQLT
                     PRALIMGMALGAFMSLSNIYIGLKAGWSMGVAITSCILAYAIFAALHRALPKQFPAFS
                     ILENNAMQSCASAAGYMTGAGLVNAIPALMMLNPQAVPGMWALTIWTLVLSWLGVFLA
                     VPAKRQMINVEQLPYPSGIAATTTMRALHSEGSKAEQQAKALMWSGILGAAITWFRDA
                     TAPWIKYPNIPATWGTNWIHIGRHRLNELTMSFEGSLLFVAAGAIMGFRQAWSMMLGA
                     CVNYLVLAPIMMDHGVIAETGGSALRRISSWSLWIGVPMMVTSGLLLFFMQWRTVVRA
                     FGTLVTFLKKRAGAADDPMERIEVPGSWFVGGMIVLGGAAIYLGHLYFHIHWWMGLIA
                     VLMTFFLVVVAARATGETDITPTGPISKITQLTFGAIQPGNVTTNLMTANITAGAVSH
                     AGDLLTDLKSGYLLGANPRQQFIAQFFGVLAGGLVVVPVFFLLVPNASVLGTEQWPAP
                     AAQVWRGVAELLSKGVGSLHPTARIGLLVGSL"
     gene            complement(1701..2570)
                     /locus_tag="VFM00_06440"
     CDS             complement(1701..2570)
                     /locus_tag="VFM00_06440"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7497919.1"
                     /translation="MPGKGFAKKGDSAASKAEERRLLTEARGGDPRALRRLLERLSPP
                     IYRFGRSFCRDSDDAQDVMQEVLVSLVRSLPSIRGGSSLTTWAYTVARRACRRQRLRA
                     ERNGAGAAGNGGLPVGAEPAAPLSADPANETERRELERALSRAIGELPPAYREAVILR
                     DVEGLSAPEAARVLGVSERALKSRLHRARLALRDVLAPHFGGGARARHAPSCVRTGRL
                     LNRYLDGELNRAACEAMQAHVESCPACGTACRALREALTLCRRPGSRRLNEETREALR
                     RAIRGALEEIGSR"
     gene            2664..3995
                     /locus_tag="VFM00_06445"
     CDS             2664..3995
                     /locus_tag="VFM00_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7497920.1"
                     /translation="MLILRRIRVGVALLGLTVLAPVAASAQTWTLDRVLEAARRSDPG
                     IAAAHASGDAGRAAAATGWSALSPRIALDAGWTRSDDPALLFSEKLWQGRFTAADFAL
                     PALNDPAPRSAWSWGATAEQPLWNGGAEITTPALAGHRARAARESARAAVADRLLEVV
                     GTYVEAVRARDALSADSTALAAAEESRRSAVERFRRGQVPELDTLRATAHWAEARVAA
                     LGADKRVQVALVRLSTTVGERVPREGLGSLPDPAALHRDGGESGPESDRRPEYVAARE
                     EARARGIEATRASLALLPSLNARADVRRYEDPESGGGARRFFVGVTASLPIWDGTRRI
                     QERRMAKARAEEAEARAELLKRTLAASSLDASVDAGVSLDRRDAANLGAVSAEEALRL
                     AQGRYRAGLLSQSDLLAVEAEAARARHARVDAESDAVLAQYRLLHARGVLE"
     gene            3992..5119
                     /locus_tag="VFM00_06450"
     CDS             3992..5119
                     /locus_tag="VFM00_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7497921.1"
                     /translation="MNRSIRRGSAPAGAALLIAALSVLAMAGCGKSQEPERANASTAP
                     PVAVTVRQVGAPGSDRALILPARVTAREEVTIRATLGARLSALPYQEGASFPAGAVLA
                     RFDATEAKAALSAARSGLAAAGSRLDLARKQEARIESLYADRVAALRELELARQDRQS
                     AEAAYAATNAAQAGLASGTEIRAPFTGVVVRHHVDPGTTLGPGDAVLDVRSTGAGEIV
                     AAVPEAWGDDLASARASIRFQTGPWHPASLLRVDGMTDYASRTRTARFRLTDRAVALV
                     PGDFAEVRLETPLATSRGAGASGTATARGALTVPSTSVIRRGSLTGVYVVQDGRAWLR
                     WVRLGREDAASAEVLAGLDAGETIALDPGKLADGRAVTARR"
     gene            5127..8384
                     /locus_tag="VFM00_06455"
     CDS             5127..8384
                     /locus_tag="VFM00_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7497922.1"
                     /translation="MKTPLGPAGRIARAFLDSKLTLLAVVASVLLGLLALLTLPREEE
                     PQIQVPVIDVTLAWPGAPVDQVERQLVTPVERRLSLIPKLEYLYSTSRDDGAVVIARF
                     EVGSNADAAWSRLRSRLDEVAATLPAGARIADVAPRSIDDVPVLALTVWDPAHPDADP
                     SDLRRVASELALELKKAQGVARVRVLGGEPRTIQVEPDPGRMAAARLTLDELMGSLAS
                     SGLSIPAGGAVRPEGWVALRAESPFTSAGEIRDLAVAARDGRPVRLSEVAAVKDEAPE
                     PTQYVIFGRHGGARYPAVTVEVTKLPGVNAMELTKQLHAQVEALRPHLIGHRLEITTM
                     RDYGETARHKSDDLMFHLLLATMSVIALIALALGRREALVVAVAIPVTLALTLFIYRF
                     LGYTLNRVTLFALIFSIGILVDDAIVVVENIARHLRLRDGRGKDQRVVEAVDEVGNPT
                     ILATVTVIAAILPLAFVGGLMGPYMKPIPIGASLAMLFSLGVAFVVSPWAARRLLVSH
                     GPAHEGPEEGKLDRAYRRVMGRLLSESRPRLFFFGLIGLLFAAAVSLVALRIVTVKML
                     PFDNKSEFEVLLDLPPGTPLERTLAAAEEVAARAAREPEVEAYQIYSGVGAPVTFNGL
                     VRHYDLRSDPRYASVEVKLVEAGKRHAQSHAIARRVRPALETVAAKYGARLKVIEVPP
                     GPPVLSTLVAEVYGPDLEGQRALARQVWGVFRTTPGVVDTDLLLAEGQPRIAMKVDRD
                     RAMLAGVSPEGVARTAAGLYAGSTVGVLQAGSETEPVPIRVRLSAADQSDESALAALR
                     VGSGAGTPLSELVRPTREAAPAEIYHKNQRRAVYVLGDVAGREESPVYAIAKLGARVD
                     SLKAPDGGPVKQLNLAMPVSTESYSLKWDGEWHITLEVFRDMGMAFAVVLVLIYILTV
                     GWFRSFTTPFVIMLPIPLSLIGILPAHAVLGAFFTATSMIGFIAGAGITVRNSIILVD
                     FIELRRRQGLPLREAVVDAGAVRFRPMVLTAAAVSVGGAVILFDPIFQGLALSLIGGE
                     IASTAISRVAVPVLYYMMRAREERARTSAAGIAARDGAGPRTVAPRETVLGEAVV"
     gene            8414..8701
                     /locus_tag="VFM00_06460"
     CDS             8414..8701
                     /locus_tag="VFM00_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497923.1"
                     /translation="MKMILMMADAERWPAIRDELTSMRVPGYSALPVIEGAGATGIHA
                     GDRVHPGGLVAVFIVEPEGAADALFDDLARRRDAAGDRVSRLFLIPVMRQA"
     gene            8718..8909
                     /locus_tag="VFM00_06465"
     CDS             8718..8909
                     /locus_tag="VFM00_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HET7497924.1"
                     /translation="MNIERALRGIAGAFVLLSVALGYYVNPWWFAFTAFVGLNLFQSA
                     FTNWCPMMAILRKAGGRQA"
     gene            8985..10436
                     /locus_tag="VFM00_06470"
     CDS             8985..10436
                     /locus_tag="VFM00_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7497925.1"
                     /translation="MSHATEGSASELAFASFLKSYPTYADTAALDGLRAREYDRLDRS
                     RHIYLDYTGGSQYAASQLRAHFELLKASVFGNPHSTSGASLAATDWLVKAREHVLRFF
                     HASPDEYFAVFTSNASGALKLVGESYPFQEGSRYLLTIDNHNSVNGIREFARARGAEV
                     EYGPIRMPDLRVDRAALSQQLGKARRDRPNLFAFPAQSNFSGVQHPLELIEEAHEAGW
                     DVLLDAAAFTPTNRFDLSRAKPDFAALSFYKMFGYPTGAGCLLLRRSAFEKLRRPWFA
                     GGAVRIASVLAEAVLRPHDESAFEDGTVDYLSLPAVQIGLEHLEQVGIDRVHERVRCL
                     TGFLLEGVEALKHSNGRPVVRVHGPTTLDDRGATVAFNVIDKNGVPLDIADVEERACS
                     ANISLRSGCFCNPGVGEVIYGLSAEQIEDLFREEGLTFDGMRARVREKWGQEVGATRA
                     SLGVATNFTDVFRFLQFLQEFRDHDSAAAGRKS"
     gene            10441..12792
                     /locus_tag="VFM00_06475"
     CDS             10441..12792
                     /locus_tag="VFM00_06475"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7497926.1"
                     /translation="MPGATPDASGKDSGRFSLVRFSVDHPRLVLGITALLTLLSLVPL
                     PKIRTDTNPKNMLPPTAPVRVRNAEVERTFVLREDMIAVGIQNDAGVLQPATLGTIYR
                     VTQAILKLPGVVSEDVTSFVTITNVRSEAGTVYVEPLMPEPPTTPEGIAQLRRKIFTN
                     PLLLDRIVSRDGRMTAIFVPIEKGANGKVIADQIRDILRREHGSERFYVAGDPVARDT
                     FGAEMFRLMAVFSPVAALVMFIAIYFMFRSFLMAASMLAVAMVAILWSIGALVALGFP
                     VHIMSSMSPVFLMAIATDSIHIFNEFMFRRREAAGRREAVLATMDAVSRPVRYTALAT
                     AAGFAVLLFMTIVPVKVFGGVIVFGTLALRLLSFSFIPAVLMLLPSGAAKAAPDEKAA
                     PGAAHGPGAMGRFVHALTATGTRRPRVVLGMVAVVLALSIAGMTRIVVNNNMIGWFKP
                     SSELRRADRAVNASLGGTSLAYIVATSARPEGFNSPEALRTLEALQARLAKLPVVGKT
                     TSVADYVKRINRELNGDDPRMETIPADAGAVGQCLFLFSSSARSTDLVNVIDDTYTKA
                     NVWVQLRTWDASAMRDVLHAVDDFKKSAPPWITFTPAGIAYFNLVWNDEVLGDMIRGF
                     ALALIAVFVILAVNFRSVRWALIGCAPLLVTILVIYGVVGWSGKQFDMPIAVLSCLSL
                     GMAVDFSIHFIGRFQQRRTDAPSEPPAEALLWTADRPGRGILRNAVLFAASFAVMLFA
                     PLTPYVTVGAFIVAMMLLSALFTLLLVPALIVTFQRALVAPTR"
     gene            12931..13884
                     /locus_tag="VFM00_06480"
     CDS             12931..13884
                     /locus_tag="VFM00_06480"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HET7497927.1"
                     /translation="MTFSAFRSFRRSLVPVSLVLGVAALGVGPLATPAARAQTAPSGT
                     EIMSRMNQAFYYPGNSFRTSVRMELGGPGTAGRERIMSLLRLNTGGKGEQRYLLYFHK
                     PGDVRRMSCMVWKHVDLPDERWMFVPITGQVVKVLAPERSSFIGSDFMREDLSGRDVD
                     ADRHTLVREEKQDGRDCYVVESLPKKPVDFSKYVTWVDRETSLPLRQEYYDNRGKLGR
                     VFTGTQIERVASAKNPSVAYPTIMDRTMKSMSSGRWTRVIFSEAVYDPSLKADDFSAD
                     HMRTPIGDWLPAQGKAVSAAASASAGKPEVAARRTPRANPP"
     gene            complement(13794..>14658)
                     /locus_tag="VFM00_06485"
     CDS             complement(13794..>14658)
                     /locus_tag="VFM00_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7497928.1"
                     /translation="TELNELRQEFAERVARVGGISVAFNATVYDDTLPYAPEVVAWAR
                     KNIDKVHVVVFIAYRQASGAFAGEMDFWVGDKKVPMNDLVYSRPTGKARLDISSRDVV
                     AEIRKRFPDFAPCAYLNGTEKADSLKWLLTTYVGTKDEVVGYAGPRFMEATQVLHHWR
                     HGRFLAYAPPSTLRHGRSITATGALFDRGMREGMARYLGGALRRPLRPLPRLHLQSVM
                     IIQPVDVLADGRMNMCDACPDMTVHEGELVWSCRLEERKAYGGFARGVRRAATSGLPA
                     EAEAAAETALP"
BASE COUNT         2048 a         5218 c         5068 g         2324 t
ORIGIN      
        1 gagcgagccc accagcagcc cgatccgtgc cgtcggatgg agcgagccca cgcccttcga
       61 caacagctcg gccacgccgc gccacacctg ggccgcgggc gcgggccact gctcggtacc
      121 cagcaccgat gcgttgggaa cgagcaggaa gaatacgggc acgacgacca ggcctccggc
      181 caatacgccg aagaactgtg cgatgaactg ctgccgggga ttggcgccga gcaggtagcc
      241 gctcttcaag tcggtcagca ggtcgccggc gtgactcacg gcgcccgcgg tgatgttcgc
      301 ggtcatcaag ttcgtcgtga cgtttccggg ctggatcgcg ccgaaagtga gctgcgtgat
      361 cttgctgatc ggcccggtcg gcgtgatgtc ggtctcgccg gtggcccgcg cggcgacgac
      421 caccaggaag aacgtcatca gcacggcgat caggcccatc caccagtgaa tgtgaaaata
      481 aaggtgcccg aggtagatcg cggcaccccc gaggacgatc atcccgccca cgaaccagga
      541 cccgggcacc tcgatccgct ccatcggatc gtccgcggcg cctgcgcgct tcttcaagaa
      601 ggtcacgagc gtcccgaacg cgcgtaccac ggtccgccac tgcatgaaga agagaagcag
      661 ccccgaggtg accatcatcg ggacgccgat ccagaggctc caggaggaga tccgccgcag
      721 ggccgagccg ccggtctcgg cgatgacgcc gtggtccatc atgatcgggg ccagcaccag
      781 atagttcacg caggcgccga gcatcatgct ccacgcctgg cggaagccca tgatcgcgcc
      841 cgccgcgacg aacaggagcg agccctcgaa ggacatcgtc agctcgttca ggcgatggcg
      901 gccgatgtga atccagttgg tgccccaggt cgccgggatg ttcgggtact tgatccaggg
      961 cgccgtggcg tcgcggaacc acgtgatcgc cgcaccgagg atccccgacc acatcagggc
     1021 cttcgcctgc tgctccgcct tgctcccctc gctgtgcagc gcccgcatcg tggtcgtggc
     1081 ggcgatcccg ctggggtagg ggagctgctc cacgttgatc atctgccgct tcgccggcac
     1141 cgccaggaac acgccgagcc acgagagcac cagcgtccag atcgtcagcg cccacatgcc
     1201 cggaaccgcc tgcgggttca gcatcatgag ggcggggatc gcgtttacga gccccgcgcc
     1261 ggtcatgtat cccgcggccg aggcgcacga ctgcatggcg ttgttctcga gaatgctgaa
     1321 ggcggggaat tgcttcggca acgcgcggtg cagcgcggcg aagatggcgt aggcgaggat
     1381 gcaggaggtg atcgccaccc ccatgctcca gcccgccttc agcccgatgt agatgttgct
     1441 gagcgacatg aacgcgccca gcgccatgcc catgatcagc gcgcgaggcg tcagctggcg
     1501 catgcggtcg ccggcgtaga cgttctcgaa ccagtggcgc tcgatctgct cgggggtttg
     1561 cggcgggggc gctgcctcta cgggacgggc cggtgcttcg gcgacggtct cggtcgtcac
     1621 ggggtttccc tccggttcac ggcggacgtg aggggggtag catgccgcgc gtgggatcgc
     1681 cgctcaacgg ctaccgttgg ctacctggag ccgatctcct cgagggcgcc gcgaatggcg
     1741 cgccggagcg cttcccgggt ctcctcgttc agacgccgcg agcccgggcg gcggcagagc
     1801 gtgagcgcct cgcgcagggc gcggcaggcc gtgccgcagg cggggcaact ctcgacgtgc
     1861 gcctgcatcg cctcgcaggc cgcgcggttc agctccccgt ccaggtagcg gttcaggagc
     1921 cggcccgtcc ggacgcagga gggcgcgtgc cgggcgcgcg cgccgccgcc gaagtgcggc
     1981 gcgagcacgt cgcgaagggc taaccgggcc cggtggaggc gcgatttcag cgcccgctcg
     2041 ctcacgccca gcacgcgggc cgcctcgggc gccgagagcc cttccacgtc gcgcaggatc
     2101 accgcttcgc gataggccgg gggcagctcg ccgatcgccc ggctcagggc gcgctccagc
     2161 tcgcggcgtt cggtctcgtt ggctgggtcc gcggaaaggg gagccgccgg ctcggctcct
     2221 acgggcaggc cgccgtttcc tgcggcgccc gcgccgttcc gttcggcccg gagccgctgg
     2281 cgtcggcagg cgcgccgcgc caccgtgtag gcccaagtgg tcagggatga gccgccccgg
     2341 atcgagggca gcgaccggac cagcgagacc aggacctcct gcatcacgtc ctgagcgtcg
     2401 tccgagtccc ggcagaaact ccggccgaag cgatagatgg ggggcgacag gcgctccaag
     2461 agccgccgca gggcgcgcgg gtccccacct ctggcttccg tgaggagtcg gcgctcctcg
     2521 gccttggagg cggctgagtc ccccttcttc gcgaatcctt tgcctggcat cgtgacactc
     2581 acccccgaat ggaccccgaa accctattcg aacacaaccc cgccccccgg gccaggggat
     2641 cccgtgcctc cacaagctgc aacatgctta ttttacggcg gatccgggtc ggggtggcgc
     2701 tcctcggcct taccgttttg gccccggtcg ccgcgtcggc ccagacctgg accctggatc
     2761 gggtcctgga ggccgcccga cggtccgatc ccggtatcgc cgccgcccac gcctcgggcg
     2821 atgccgggcg cgcggccgcc gccacgggct ggtccgcgct gtccccgcgg atcgccctgg
     2881 acgcgggctg gacgcggagc gacgatcccg cgctcctctt ctcggagaaa ctctggcaag
     2941 gccgcttcac ggctgcggac ttcgcccttc cggccctgaa cgaccccgcg ccgcgcagcg
     3001 cctggagctg gggcgcgacg gccgagcagc ccctctggaa tggcggcgcc gagatcacga
     3061 cgccggccct ggcagggcat cgcgcccgcg ccgcgcgcga atcggcgcgc gccgcggtcg
     3121 cggaccggct cctggaagtg gtggggacct atgtcgaggc ggtccgggcg cgcgacgccc
     3181 tttcggccga ttccaccgcg ctcgcggccg cggaagaatc gcgccggtcg gccgtggagc
     3241 gtttccggcg cggccaggtt cccgagctgg acacactgcg cgccacggcg cactgggcgg
     3301 aggcgcgtgt cgcggcgctc ggcgccgaca agcgcgtcca ggtcgcgctc gtgcggctct
     3361 cgaccacggt gggggagcgg gtgccccgtg agggcctcgg gtcgcttccg gatcccgccg
     3421 cgctccatcg cgacggcggc gagagcgggc ccgagtcgga ccgccgtccc gaatacgtcg
     3481 ccgcccgtga ggaagcgcgt gcgcgtggga tcgaagccac ccgagcctcg ctcgccctcc
     3541 ttccctccct gaacgcgcga gcggacgtcc gccgatacga agacccggaa tcgggtgggg
     3601 gagcccgacg tttcttcgtg ggggttaccg cttcgctccc gatttgggac ggcacgcggc
     3661 gaattcagga gcgccgcatg gccaaggccc gcgccgaaga ggctgaagct cgcgcggagc
     3721 tcctcaagcg cacgctcgcc gcgtcctccc tggacgcttc cgtcgacgcc ggcgtctcgc
     3781 tcgaccgccg cgacgctgcg aatctcgggg ccgtatccgc cgaggaggcg ctccgcctgg
     3841 cgcagggccg ttatcgcgcg ggacttctct cgcaatccga tcttctggcc gtggaggccg
     3901 aggccgctcg cgcgcgccat gcccgcgtcg acgccgagtc cgatgccgtc ctggcccagt
     3961 accgtcttct gcatgcacgg ggggtgctcg aatgaaccga tccatccgcc gcggctccgc
     4021 tccggcgggc gccgcccttc tgatcgccgc gctctccgtg ctcgccatgg ccggctgcgg
     4081 caagagccag gaaccggagc gcgcgaacgc gagcaccgcc ccgccggtcg cggtgaccgt
     4141 gcgccaggtg ggcgcccccg gctccgaccg ggcgctcata ctccccgcgc gcgtcacggc
     4201 gcgcgaagag gtcaccatcc gcgccaccct cggcgcccgc ctgagcgcgc tcccgtatca
     4261 ggagggcgct tccttcccgg ccggcgccgt gctcgcgcgc ttcgacgcga ccgaggcgaa
     4321 ggcggcgctg tccgccgccc gttccggact cgccgcggcc ggctcacggc tcgatctcgc
     4381 ccggaagcag gaggcgcgga tcgagtcgct gtacgccgac cgcgtggccg cgcttcgcga
     4441 gctggagctg gcgcgccagg accggcagtc cgccgaggcc gcgtacgcgg cgacgaacgc
     4501 cgcgcaggcc ggtctcgcct ccggcaccga gatccgcgcg cccttcaccg gagtcgtcgt
     4561 gcgccaccac gtcgatccgg gaaccacgct ggggccgggg gacgccgtgc tcgacgtgcg
     4621 gtccaccggc gccggcgaga tcgtggccgc ggtgcccgaa gcctggggcg acgacctcgc
     4681 ctccgcgcgg gcctccattc gattccagac cgggccgtgg caccccgcgt cgctgcttcg
     4741 ggtagacggg atgaccgact acgcgagccg cacgcgcacg gcccggtttc gactgaccga
     4801 ccgcgccgtc gcgctcgtcc ccggcgactt cgccgaagtg cgtctggaaa cgccgctcgc
     4861 gacctcccgc ggcgccggcg cctccgggac cgcgacggcg cggggtgccc tcaccgttcc
     4921 ctccacttcc gtgatccggc gcggatcgct caccggcgtc tacgtcgtcc aggacggccg
     4981 cgcgtggctg cgatgggtgc ggctcggtcg cgaggacgcc gcgagcgcgg aagtgctcgc
     5041 cggtctcgac gccggcgaaa cgatcgccct cgatcccggc aagctcgccg acggccgcgc
     5101 cgtcacggcg cggcggtgat ttcgcggtga agacgcccct gggtcccgcc gggcggatcg
     5161 cccgcgcatt cctcgactcg aagctgacgt tgctcgccgt cgtcgcatcg gtgctcctcg
     5221 gtctcctggc cctgctcacg ctgccgcgcg aagaggagcc gcagatccag gtacccgtca
     5281 tcgacgtgac cctggcgtgg cccggcgctc ccgtggacca ggtggagcgc cagctggtca
     5341 cgcccgtcga gcggcggctc tccctgattc cgaagctcga atacctctac tcgacgtcgc
     5401 gggacgacgg cgccgtcgtg atcgcgcgat tcgaggtggg gtcgaatgcc gacgcggcgt
     5461 ggagccggct gcgctcgcgc ctggacgagg tcgccgccac gctccccgcg ggagcccgca
     5521 tcgccgacgt cgcgccgcgc tccatcgacg acgtgccggt cctggccctg accgtgtggg
     5581 atccggcgca ccccgacgcc gacccgagcg acctgcgccg cgtggcctcc gagctcgcgc
     5641 ttgagctgaa aaaggcgcag ggcgtggcgc gcgtccgggt cctgggcggc gagccgcgca
     5701 cgatccaggt cgagccggat cccggccgca tggcggccgc gcgcctcacg ctggacgagt
     5761 tgatggggag cctggcctcg tccggcctct cgattcccgc cgggggcgcc gtccgtcccg
     5821 agggatgggt cgcgctccgg gcggagtcgc cgttcacctc ggcgggggag atccgggatc
     5881 tggccgtggc ggcgcgcgat gggcgcccgg tacggctctc cgaggtggcc gcggtgaagg
     5941 acgaggctcc cgagcccacg cagtacgtga tctttggccg gcacggcggc gcccgctatc
     6001 ccgccgtcac ggtcgaggtc acgaagctcc ccggcgtgaa cgcgatggag ctgacgaagc
     6061 agctccacgc gcaggtggag gcgctccgcc cgcacctgat cgggcaccgg ctcgagatca
     6121 cgacgatgcg ggactacggg gagaccgcgc gccacaagtc ggacgacctg atgttccacc
     6181 tgctcctcgc gacgatgtcg gtcatcgcgt tgatcgcgct ggcgctggga cgccgcgagg
     6241 cgctggtcgt cgcggtcgcc atcccggtca cgctcgcgct cacgctcttc atctaccgct
     6301 tcctgggcta cacgctgaac cgggtgacgc tgttcgcgct catcttctcg atcggcatcc
     6361 tggtcgacga cgcgatcgtc gtggtcgaga acatcgcgcg gcacttgagg cttcgggatg
     6421 gacgcggcaa ggaccagcgg gtggtcgagg cggtggacga agtgggcaat ccgaccatcc
     6481 tcgcgaccgt gacggtgatc gccgcgatcc ttccgctcgc cttcgtgggc gggctcatgg
     6541 gcccctacat gaagccgatc ccgatcggcg cgtcgctggc gatgctgttc tccctgggcg
     6601 tggcgttcgt cgtctcgccg tgggcggccc ggcgcctcct cgtgagccac ggccccgcgc
     6661 acgagggtcc cgaggagggg aagctggatc gcgcgtatcg ccgcgtgatg gggcggctcc
     6721 tttccgaatc acgcccgcgg ctcttcttct tcgggctgat cgggcttctc ttcgcggccg
     6781 cggtttccct ggtcgcgctc cggatcgtga ccgtgaagat gctgcccttc gacaacaaga
     6841 gcgagttcga ggtgctgctc gacctcccgc cgggaacgcc gctggagcgg acgctcgccg
     6901 cggccgaaga ggtcgccgcg cgcgccgcgc gcgagccgga agtcgaggcc taccagatct
     6961 actcgggcgt cggcgcgccg gtcacgttca acggactggt gcgccactac gatctgcgct
     7021 ccgatccgcg ctacgcctcg gtggaggtga agctggtcga ggcggggaag cggcatgcgc
     7081 aaagccatgc catcgcgcgg cgcgtgcgtc cggcgctgga gacggtggcc gcgaagtacg
     7141 gcgcgcggct caaggtgatc gaggtgccgc ccggccctcc ggtgctctcg acgctggtgg
     7201 ccgaggtcta cggtcccgac ctcgaagggc agcgcgccct cgcgcggcag gtgtggggcg
     7261 tcttccggac cacgccgggc gtcgtggaca ccgacctgct cctggccgag gggcagccgc
     7321 ggatcgcgat gaaggtggac cgcgaccgcg ccatgctcgc cggcgtctcc ccggagggag
     7381 tggcccgcac ggcggcaggg ttgtatgccg ggagcaccgt gggcgtcctg caggccgggt
     7441 ccgaaaccga gccggttccg atccgggtgc gcctgtcggc cgcggatcag tccgacgagt
     7501 cggcgctcgc cgcgctccgc gtcggaagcg gcgcggggac gccgctctcc gagctggtgc
     7561 gcccgacgcg ggaggcggcg ccggccgaga tctaccacaa gaaccagcgg cgcgcggtgt
     7621 acgtgctcgg ggacgtggcg gggcgcgagg agagccccgt ctacgcgatc gcgaagctgg
     7681 gcgcgcgcgt cgattcgctg aaggcgcccg acggcggtcc ggtgaagcag ctgaatctcg
     7741 ccatgccggt gtccacggag tcctactcgc tcaagtggga tggcgaatgg cacatcacgc
     7801 tcgaagtgtt ccgcgatatg ggcatggcct tcgccgtcgt gctcgtgctc atctacatcc
     7861 tcacggtggg gtggttccgc tccttcacga ctccgttcgt gatcatgctg ccgatcccgc
     7921 tgagcctcat cggaatcctt cccgcgcacg ccgtgctggg cgcgttcttc accgcgacct
     7981 cgatgatcgg attcatcgcc ggggccggca tcaccgtgcg gaactcgatc atcctcgtcg
     8041 atttcatcga gctgcgccgc cgccagggcc tgccgctgcg cgaggcggtg gtggacgcgg
     8101 gcgccgtgcg cttccggccg atggtgctga cggcggccgc cgtgtcggtg ggcggcgcgg
     8161 tgattctgtt cgacccgatc ttccaggggc tcgccctctc cctgatcggg ggggagatcg
     8221 cctcgaccgc catctcgcgc gtggcggtgc cggtcctgta ctacatgatg cgggcgcgcg
     8281 aggagcgagc ccggacgagc gcggcgggga tcgccgccag ggacggggcg ggcccgcgca
     8341 cggtggcccc ccgggaaacc gttctcggag aggccgtcgt atgagcggag tcggcgggcc
     8401 aggaggagcg gggatgaaga tgatcctcat gatggcggat gccgagcggt ggccggcgat
     8461 tcgcgacgag ctgacttcga tgcgcgttcc cggctacagc gcgctccccg tgatcgaggg
     8521 ggcgggcgcg acgggaatcc atgctggcga ccgggtccat ccgggcggat tggtcgccgt
     8581 cttcatcgtg gagcccgagg gcgcggcgga cgccctgttc gacgatctgg ctcggcgacg
     8641 tgacgccgcc ggcgatcgtg tgagccgcct gtttctcatt ccggtcatgc gccaggcgtg
     8701 acccgatcgg aggagtcatg aacatcgagc gcgctctgcg cggcatcgcc ggagctttcg
     8761 ttctgctctc ggtggctctg gggtattacg tcaatccgtg gtggttcgcc ttcaccgcct
     8821 ttgtgggcct gaatctgttc cagtcggcgt tcacgaactg gtgccccatg atggccattc
     8881 tgcgcaaggc gggggggcgc caggcctagt ccgcgcggga gacgtgcctg tggtatgatg
     8941 atgcggttcg ccgcccactg accggccaat tgcgggtgcc attcatgagc catgcgaccg
     9001 agggctccgc gagcgagctc gcgttcgcgt cgttcctcaa gagctacccc acgtacgccg
     9061 acaccgccgc cctcgacggg ctgcgggcgc gcgagtacga ccgcctggat cgcagccgcc
     9121 atatctacct cgactacacg ggcggcagcc agtacgccgc cagccagctt cgcgcccact
     9181 tcgagctcct gaaagcgtcc gtcttcggga atccgcactc cacgagcggc gcctcgctgg
     9241 ccgcgaccga ctggctggtg aaggcgcgcg agcacgtgct ccgcttcttc cacgcctctc
     9301 cggacgagta cttcgccgtc ttcacgtcga acgcgagcgg cgcgctcaag ctggtcggcg
     9361 agtcgtatcc cttccaggag ggcagccgct acctgctcac gatcgacaat cacaactccg
     9421 tgaacggcat tcgcgaattc gcgcgcgccc ggggcgccga ggtggagtac ggaccgatcc
     9481 ggatgccgga tcttcgcgtg gaccgcgccg cgttgtcgca gcagcttggg aaggcccggc
     9541 gcgaccgccc gaacctgttc gcgttcccgg cccagtccaa cttctccggc gtccagcatc
     9601 cgctggagct gatcgaggag gcgcacgagg cggggtggga cgtgctgctg gacgccgcgg
     9661 cgttcacgcc cacgaaccgc ttcgacctct cgcgggccaa gcccgacttc gccgcgctct
     9721 cgttctacaa gatgttcggc tatcccacgg gggcggggtg cctgctcctg cgccggtccg
     9781 ccttcgagaa gctgcgccgc ccctggttcg cggggggcgc cgtgcgcatc gcgagcgtcc
     9841 tcgccgaggc ggtgctccgc ccgcacgacg agtcggcttt cgaggacggc acggtggact
     9901 acctgagcct tcccgccgtc cagatcggac tggagcacct ggagcaggtg gggatcgacc
     9961 gcgtccacga gcgcgtccga tgtctcacgg gcttcctcct cgaaggggtg gaagccctga
    10021 agcactccaa cggccgcccc gtcgtgcgcg tgcatggacc gaccacgctg gacgatcggg
    10081 gggcgacggt ggccttcaac gtgatcgaca aaaacggcgt cccgctcgac atcgcggacg
    10141 tcgaagagcg cgcctgcagc gccaatatct cgcttcggtc cggctgcttc tgcaaccccg
    10201 gggtcggcga agtcatctac ggcctctccg ccgagcagat cgaggacctc ttccgggagg
    10261 aaggcctcac gttcgacggc atgcgcgccc gcgtgcgcga gaaatggggc caggaggtcg
    10321 gcgccacgcg cgcctccctc ggcgtcgcga ccaacttcac ggacgttttc cgcttcctcc
    10381 aattcctgca ggagttccgc gaccacgact cggccgccgc cggccggaaa tcctgacgcg
    10441 gtgcccggcg ccacgcccga cgcgtccggc aaggacagcg gccgcttctc gctcgtccgt
    10501 ttctccgttg atcacccccg actcgtcctg gggatcaccg ccctcctgac gctgctttcg
    10561 ctggttccgc tcccgaagat ccgcaccgat accaatccca agaacatgct cccgcccacc
    10621 gcgcccgtgc gcgtccggaa cgcggaggtg gagcggacgt tcgtcctgcg cgaggacatg
    10681 atcgcggtcg ggatccagaa cgacgccggc gtgctccagc ccgcgacgct gggcacgatc
    10741 taccgggtca cgcaggccat cttgaagctg cccggcgtcg tttccgagga cgtcaccagc
    10801 ttcgtcacga tcaccaacgt gcggagcgag gcggggacgg tctacgtgga gccgctgatg
    10861 cccgagccgc cgaccacgcc cgaagggatc gcgcagctgc gccggaagat cttcaccaac
    10921 ccgctcctcc tcgaccggat cgtttcgcgc gatggccgga tgaccgcgat cttcgtgccc
    10981 atcgagaagg gcgcgaacgg caaggtgatc gcggaccaga tccgggacat cctgcgccgg
    11041 gagcacggct ccgagcgctt ctacgtggcg ggggaccccg tggcccgcga cacgttcggc
    11101 gccgagatgt tccggctgat ggcggtcttc tcgccggtcg ccgcactcgt gatgttcatc
    11161 gcgatctatt tcatgttccg gagcttcctc atggccgcat cgatgctggc cgtggccatg
    11221 gtggcgatcc tctggagcat cggcgccctg gtggcgttgg gcttcccggt gcacatcatg
    11281 agctcgatga gcccggtctt cctgatggcg atcgccaccg acagcatcca catcttcaac
    11341 gagttcatgt tccgtcggcg tgaggcggcc ggacgccgcg aggcggtgct cgccacgatg
    11401 gacgcggtgt cgcggccggt gcgctacacg gcgctcgcca ccgccgcggg cttcgccgtg
    11461 ctcctgttca tgacgatcgt gccggtgaag gtgttcggcg gggtcatcgt cttcgggacg
    11521 ctggcgctgc ggcttctgtc gttcagcttc attccggcgg tgttgatgct attgccgtcc
    11581 ggtgcggcga aagccgcgcc ggacgagaag gcagccccgg gtgccgcaca cggccccgga
    11641 gcgatgggcc gcttcgtgca tgccctgact gccaccggaa cccggcgtcc ccgtgtcgtg
    11701 ctcggcatgg tcgccgtcgt gctggccctc tccatcgccg gcatgacccg gatcgtcgtg
    11761 aacaacaaca tgatcggatg gttcaagccc tcgagcgagc ttcgccgggc cgaccgcgcc
    11821 gtgaacgcgt cgctcggcgg gacgtccctc gcctacatcg tggcgacgtc ggcccgcccc
    11881 gaggggttca actctccgga agcgctgcga accctggagg cgctccaggc ccgccttgcg
    11941 aagctgccgg tcgtgggcaa gacgacgtcg gtcgccgact acgtgaagcg gatcaaccgc
    12001 gagctgaacg gcgacgatcc gcgcatggag acgatccccg ccgatgccgg ggccgtgggg
    12061 cagtgcctct tcctcttcag ctcctcggcg cgttccaccg atctcgtgaa cgtgatcgac
    12121 gacacctaca ccaaggcgaa cgtctgggtg cagctcagga cctgggacgc gagcgcgatg
    12181 cgcgacgtgc tccacgcggt cgacgacttc aagaagagcg cgcccccgtg gatcaccttc
    12241 accccggccg gcatcgccta cttcaacctg gtctggaacg acgaggtgct gggggacatg
    12301 atccgcggct tcgcgctcgc gctgatcgcg gtcttcgtga tcctggccgt gaacttccgc
    12361 tcggtgcgct gggcgctgat cggctgcgcc ccgctcctcg tcacgatcct cgtgatctac
    12421 ggcgtggtgg gctggtcggg gaagcagttc gacatgccga tcgcggtgct ctcctgcctc
    12481 tcgttgggca tggcggtgga cttctcgatc cacttcatcg ggcgcttcca gcagcgccgg
    12541 acggacgcgc cgtcggagcc gccggcggag gcgctcctct ggaccgccga ccggcccggg
    12601 cgggggatcc tccgcaacgc cgtcctcttc gccgcctcct tcgcggtgat gctcttcgcc
    12661 ccgctcaccc catacgtcac cgtcggcgcc ttcatcgtcg cgatgatgct cctgagcgcg
    12721 ctcttcacgc tgctgctcgt cccggccctg atcgtcacgt tccagcgcgc gctggtagcg
    12781 ccgacccgct gatcggtccc ctgcggaagc gctcttttgg gactctccct ccggcttcgc
    12841 gtgttagact tgtcacacga cctcgcggac agcatccgcg gcgtctgctc cagtcggcta
    12901 aatcacgatt tcgcttcacc ttggagtcgc atgaccttta gcgccttccg ctccttccga
    12961 cgctctctcg tcccggtttc cctcgtcctg ggggtggctg cgcttggcgt gggcccgctc
    13021 gccacgccgg cggcgcgcgc ccagaccgcg cccagcggaa ccgagatcat gtcgcgcatg
    13081 aaccaagcct tctactaccc gggcaacagc ttccggacgt cggtgcggat ggagctgggt
    13141 ggtccgggca ccgcgggccg cgagcggatc atgagccttc tccgcctgaa cacgggcggc
    13201 aagggggagc agcgctacct gctctacttc cacaagccgg gcgacgtgcg gcgcatgtcc
    13261 tgcatggtct ggaagcacgt ggatcttccg gacgagcgct ggatgttcgt gccgatcacg
    13321 gggcaggtcg tgaaggtgct cgcgcccgag cgctcgagct tcatcggctc ggacttcatg
    13381 cgcgaggatc tctcggggcg ggacgtggac gccgaccggc atacgctggt ccgcgaggag
    13441 aagcaggatg ggcgggactg ctacgtcgtg gagagcctgc cgaagaagcc cgtggatttc
    13501 agcaagtacg tcacctgggt ggaccgcgag acgtcgctcc cgctgcggca ggagtactac
    13561 gacaatcgcg ggaagctggg acgcgtgttc accgggacgc agatcgagcg ggtcgcgagc
    13621 gcgaagaacc cctcggtcgc ctacccgacg atcatggacc gcacgatgaa gtcgatgtca
    13681 tcggggcgat ggacgcgcgt gatcttcagc gaggccgtct acgatcccag tctcaaggcc
    13741 gacgacttct ccgccgatca catgcgcacg ccgatcggcg actggctgcc cgctcagggc
    13801 aaggccgtct cggccgcagc ctccgcctcc gccggcaagc ccgaagtcgc cgcgcgccgc
    13861 acgccgcgcg cgaacccgcc gtaggccttc cgctcctcca gcctgcagga ccagaccagc
    13921 tctccttcgt gcaccgtcat gtcggggcac gcgtcgcaca tgttcatccg gccgtccgcc
    13981 agcacgtcca ccggctggat gatcatcacg gattgcagat ggagccgcgg cagcggccgg
    14041 agcggccggc gcaaggcgcc ccccaggtag cgggccatcc cctcgcgcat cccgcggtcg
    14101 aagagggcac ccgtggcggt gatcgagcgc ccgtgccgca gcgtggaggg aggcgcgtac
    14161 gcgaggaagc ggccgtgccg ccagtggtgc agcacctggg tggcttccat gaagcgcggc
    14221 cccgcgtagc ccaccacctc gtccttcgtc cccacatacg tcgtgagcag ccacttcaac
    14281 gagtctgcct tctcggttcc gttcaggtag gcacaggggg cgaagtcggg gaagcgcttc
    14341 cggatctcgg ccacgacgtc gcgtgaggag atgtcgaggc gggccttccc ggtggggcgg
    14401 ctgtagacca ggtcgttcat cggcaccttc ttgtcgccga cccagaagtc catctctccc
    14461 gcgaacgctc ccgacgcctg ccggtaggcg atgaacacca cgacgtgcac cttgtcgatg
    14521 ttcttccggg cccacgccac cacctccggc gcgtagggca gcgtgtcgtc atagaccgtc
    14581 gcgttgaacg ccacggagat cccgcccacc cgggcgacgc gctcggcgaa ttcctggcgc
    14641 agctcgttca gctccgtc
//
LOCUS       DASSLZ010000168        26297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1451730, whole genome shotgun sequence.
ACCESSION   DASSLZ010000168 DASSLZ010000000
VERSION     DASSLZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 26297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26297
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451730"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFM00_06490"
     CDS             complement(<1..527)
                     /locus_tag="VFM00_06490"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HET7497929.1"
                     /translation="MTKTSVAVFGSSKAARDSPHHRLARELGAALARRGAVVRCGGYG
                     GVMEAVTEGATSAGGKVVGCTLAWYQETRVPSSELSEVVEAADLYERIRNLLAGARAA
                     VVLPGGVGTLNELFWVWTLLTHDRDEDPDALVLLGEGWDELLDFLDRRFEFGPDLRGL
                     LRVAHDAEEAARIAWG"
     gene            624..2411
                     /locus_tag="VFM00_06495"
     CDS             624..2411
                     /locus_tag="VFM00_06495"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET7497930.1"
                     /translation="MTPQRAARAHTSRSRASRIVRIALAQVNPTVGNLPGNTHKVIEF
                     IGRAREMGAELVAFPELVLTGYPPEDLLLRPEFIDQNMAALDEVTRATRGITAVVGFA
                     QRAEDVYNAAAIAHDGEIAGIYHKSHLPNYSVFDEVRYFRPGRNPFVCRRGALTFGVN
                     ICEDIWLPSSLNQLAVYGDADLVINLSSSPYHMGKSIARDRMLATRAQDAVSVLAFVN
                     TVGGQDELVFDGNSRVFGPRGTTLAAAKAFEEDLVVLDIDLDEVFSARLKDPRSRELA
                     HRDEPEEPIPVVELRPVPDPPRPPIQARATVTPAGEAEVYEALILGTRDYVRKNGFEH
                     VVLGLSGGIDSALTASVAVDALGAKNVTGAYMPSGFSAKESEADATALAKNLGFPMLT
                     LPIAAEMEAYRKSLAPVFEGRPEDVTEENIQSRIRGNFLMALSNKFGWLVLTTGNKSE
                     YATGYTTLYGDMAGGFAVLKDVPKTLVYALARHRNARAGKGAWIPERTIERPPTAELR
                     AGQLDTDSLPPYDVLDPILRAYVEEDWEVDRIVGMGYDRALVQRIIRMVDKAEYKRRQ
                     APPGIRITPRALGKDRRLPITNGHQSWRD"
     gene            complement(2679..3326)
                     /locus_tag="VFM00_06500"
     CDS             complement(2679..3326)
                     /locus_tag="VFM00_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7497931.1"
                     /translation="MHAVDPLSLFHHRFVPPEPNGSPLLLLLLHGTGGDEDDMLPLGP
                     YLAPGAAILSPRGRVLENGAPRFFRRIAEGVFDEEDLKHRADELAQFVHAARDRYGIA
                     AQPMVAVGFSNGANIAGALLLLHPDLLERAILLRGMTPFTPEVPPALTGKRVYLGAGR
                     NDPLVRPYASEKWAELLRGAGADVTLQWSDTGHALGQEDIDLARAWLNNEATAGR"
     gene            complement(3468..5957)
                     /locus_tag="VFM00_06505"
     CDS             complement(3468..5957)
                     /locus_tag="VFM00_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497932.1"
                     /translation="MIRRCTGGPAPIAGPLLRRAARAAARAISCAAVTLALAGVPRPA
                     GATDWIPPARITALLPSAVIAPGVTRPLTLTLRANGSLANVAWAATSGGAFVATLSPS
                     SGVLSVPANSIASVTINVTVPALAVGSSSISVDILADPGGARLAKVGASITAASDGRP
                     EVWPSPSTWSAASGTSGSVSFQVHSMTGTSETVLLTGGRFNPDPNNAGGLFAGSPPPA
                     SALLPGGGTITVNVPTTIPARSFAGNGNAVQLSVTNSGGVSNASGFALSSASLPDSLP
                     TALVPMGLTPLSAAAAGRDGAALLASRGYWLVPSGEDGVRVIRTTATDSIGMIDGNGD
                     GGDDRVVGTIRIPSYAAALSILPGFVTTSGDTLDLGLLAAGRGGLMLLDLRTIEDPLF
                     GDWSDFFDVDRNGVDDRILRVIPTSGFATDAAWVRTATGRHVGLVADADTGSIPVAAS
                     YDPALVISGTGAGVVAVDLEAAIDSLGGVPYAAGTLPTPGSALDLELRGGGSAPEMAL
                     ADGSGGVAFYGLTFAPGAPASVTFTPRGNVALSGMWGSALARDLAWVANTGDSAYVAV
                     ATGAGGVQLVRAPRGGQPPALVLVQQTVAPAVGLASAWTGTLGVAMEGGGVSLLRVPG
                     ASELDKIQPGAPAPYTQPVSLSRVQSWGASGGLERALHVSWATSSTSLRFRDMPGFLP
                     DLVVSDGARLLVLRSGTAAISGVAVAASPAPAVPALRVAPNPSSGAVWLEASRSAVAL
                     AEARSGGAGAAGSVGSAGGAGSTGGAIRIAITDVRGRLVRTLRAEGDATRVRWDGRDD
                     RGRPAASGRYWARLLVPGNVRTPAASITILR"
     gene            complement(5982..8693)
                     /locus_tag="VFM00_06510"
     CDS             complement(5982..8693)
                     /locus_tag="VFM00_06510"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET7497933.1"
                     /translation="MNSIRFRIAAAVLVFGALALTLANPAAAFIRLARQADATSPVVQ
                     AHWFDSELPLNSVINPANADISSTDALATVIQSAKNWENVNTSYFTVNPHEFGATDSL
                     PKLAFDGQNSMFFDTPGANFAVGSGVIAFVRSVVDLSNGHTLDADLVFNDHEFFSSIS
                     SPGLTPAPAGQTSVDLQAVVTHEYGHYFGLDHTSVAGATMVPFISNDISQRTLELDDR
                     AGISTIFPESADRGLSADGVDFNATTGTISGTVLNGFNNLALFGAHVEAYNLAVPDAA
                     HSISAISGELTLRNGQGEYTIHGLPPGSYAVRIVPLDGVNTIAADANIGGPYNGLDIG
                     FEPEFWNGANESGNGFTDLANSFDPVTVNAGTNTPGVNFISNTFPGRVIIAQHGAFEN
                     IVTFANNAYLAVRFDPPFEGPYTIANVSFPSFTFNGVPALFPQILLCPMSLVTGGPDL
                     ANPLFVQTLYAGNPNGNNVVPLNMPVNTPNQTFFWVIRFPSQAATPGFPNNFPFIRAD
                     FTTLEKGLFANSYSVQLSGASGVLIDRNLAVDMTCQLPDPNAVPIVAPTGLGANRRAT
                     QTEFTYVGSANRRADGFDLPQNSVDQTFLIQRSPFAYADRDTAGAGAAAFHLSPSPPS
                     TGGATIWSSQSLDKNGHRSLQSAVTILALNEDADEPNGRLNEAKVLPGNVTNRAETYS
                     PAGDQDFYSVLAKPGDIIDASATATGQDGRNNTDLVMFLYDSNGDVVAFNDDFTGLNP
                     RVVFTAPPNPGNSKNARKFTVQVTDFRSSTLTNPVPQVRVPQTYVLSASVTTPPAAAA
                     RIAGRTIDPDQFFFANSGPNPANPVAKFLFVIPRNAGNAAVRLNIFDVTGRLVRTLVD
                     GKVMEAGPHTTLWDGRDVAGRTVASGTYFARMESGSWHQDARVTILK"
     gene            complement(8710..9306)
                     /locus_tag="VFM00_06515"
     CDS             complement(8710..9306)
                     /locus_tag="VFM00_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497934.1"
                     /translation="MNRASRLAMVAFAALALVGTSTAHATTVTKMSLKDLALKSNAVV
                     VARVEDQVARYDANKEIYTYVTLRVLDPVKGSRKDDVITIRQLGGVVDNIASIVPGMP
                     TFKKGEEVVVFLSQNDKAGYPWIMGLQQGKYTVSQDQNGTKRVRNELEGTSLIDASGK
                     AFEPGKTADMPLQALLDGVRTNLDEAGKIQIDPTVPNE"
     gene            complement(9627..10529)
                     /locus_tag="VFM00_06520"
     CDS             complement(9627..10529)
                     /locus_tag="VFM00_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET7497935.1"
                     /translation="MPSPADDYRALREDAAALDLGDWRVLRLSGADTREFLQGTASQD
                     LDAAGPERAAETLFLTEKGRPVAHAWVAVAASGADAWVLADAASAEALRPHLERFRIM
                     EDVEFEGPATTPRLVGVAGPNRSGLLASLAGRAAGATAIAGAPLSFLFLPAATALPAF
                     AAREAADAWRIAAGIPVAGADFDLDRIATELDLPGAISFTKGCYVGQEVVARTSNRGQ
                     VRRRRAGFRFPWPGSAPAKGTPILAGGSPAGFLTSAAPEPGTGEGLGMGYLSTDRLDA
                     DGTADFSLSDPSGGRSIRIAAWPL"
     gene            10661..10984
                     /locus_tag="VFM00_06525"
     CDS             10661..10984
                     /locus_tag="VFM00_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7497936.1"
                     /translation="MPRSRRTGAPLDVELARLAKALGHPARVAIVRLLLGKKECVCGD
                     IVDRLPLAQATVSQHLKVLKDAGWIQGSIDGPRVCYCARPETGRKFLELAQALAAREE
                     EGETS"
     gene            10981..11784
                     /locus_tag="VFM00_06530"
     CDS             10981..11784
                     /locus_tag="VFM00_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HET7497937.1"
                     /translation="MSATNDIRDTVRERYGALAEGADCGCGCSSGTVSCCSTDSQAMA
                     TLGYTAEQLAAIPEGANLGLGCGNPLAHAAVKPGETVLDLGSGAGIDAFLAAREVGAE
                     GRVIGVDMTPSMISRARENAAKGDYRNVEFRLGEIENLPVADGSVDVIISNCVINLSP
                     DKPRVFAEAFRALKPGGRLVVSDLVLTRPIPDAVRKSIEAYVGCIAGASSREDYLRLV
                     REGGFDDVTVLEERGYGQDGPLPTVVIEERAWDAVASVKVRGVKPPNRT"
     gene            complement(11785..12711)
                     /locus_tag="VFM00_06535"
     CDS             complement(11785..12711)
                     /locus_tag="VFM00_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET7497938.1"
                     /translation="MASRRWAIILAAGDGERVRRLTMDGRGRPLPKQYWAPGDAGPML
                     RWAFDRAKRLTRPAHIVTVVAAHHREWWGDLVPGSMIPGMMVQPANRGTGAGILLPLL
                     RVLRKSRDAVVVVLPADQYVADESTLASSLARAVSVVERGRRVVVLGMAPQGPDPDYG
                     WIVGGEAEEDGTRPIVSFVEKPTPRMARELYRSGASVSSFIVVARGRALLALFQTAAP
                     ALLASLARVTEEREPGAVPDRAALERAYEALPHVDFSRDVLQGSAAHLRVLAVPPCGW
                     VDLGTPERLERWIRSRGEGDAGQHAVLHKTTA"
     gene            complement(12900..13484)
                     /locus_tag="VFM00_06540"
     CDS             complement(12900..13484)
                     /locus_tag="VFM00_06540"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET7497939.1"
                     /translation="MNWTPPDTAEAPLTAWVRDVPDWPKKGILFRDLTPLWADGHAWA
                     RAVRALAAPFDAAPPAMVLGIEARGFLIAAALAARWNAGLLLARKEGKLPASAHREEY
                     DLEYGRACLETHRDLVPAGTRVVIADDVMATGGTARAALDLVRAIRCEPAGFAFLVEL
                     SFLDGRSKIDRAIPVHSVIAYDADGRASVRESLP"
     gene            13538..14464
                     /locus_tag="VFM00_06545"
     CDS             13538..14464
                     /locus_tag="VFM00_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HET7497940.1"
                     /translation="MHVLVSGSSGFLGSALMPRLAAAGHRVTRLVRSRGGTEGGASGR
                     GMAEVRAWAPDADALDPRVLEGIDAVIHLGGASIGSVWTSRRKELLRTSRVRTTRLLA
                     ERIASASPRPAVFAHASAVGYYGDRGDEVLTEASAPGRGFLADLCRDWEAASMPAQDA
                     GARVVRVRAGLVLSPRGGVLPVMLRPFRLGLGGRLGSGRQWMPWIALEDAVAVYLRAL
                     EDGALVGAVNAVAPEAVTNAEFTRSLARAVRRPSFVTVPAFALGLLPGGMGKETLLAS
                     ERVVPKALQTAGFRFESPTLETALSAIRGGGP"
     gene            complement(14418..15317)
                     /locus_tag="VFM00_06550"
     CDS             complement(14418..15317)
                     /locus_tag="VFM00_06550"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET7497941.1"
                     /translation="MSSHKEHKEEMIELCAAYALGTIEEKDRKRLEAHLAEGCPECET
                     ALREFGYGATLLAASAPRVLPLPGLRERALAAVTSEQGAAEHGTRDRGAAVPGGSGAP
                     GAAKVLRLPRTGRPGWFAWTGWIVAAVMIISSGTFYESTGRLRAILRAREADLNGTAR
                     DLAVERQWGGVLTSPIARVADLAPAAPLAGETSGIRGRAVYDPATRRAVVVLTNATAP
                     DSQSFQLWAVRGLSTTSMGVIHPDEHGAAVLRLESAGEPTTLTAFAVSLEPKGGSPDK
                     TAPSGPVVLMGPLRESPRGPSRG"
     gene            complement(15314..15865)
                     /locus_tag="VFM00_06555"
     CDS             complement(15314..15865)
                     /locus_tag="VFM00_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7497942.1"
                     /translation="MSESDFELIRRLQEGDRDAVGSLYDRYAPLLYPLMVRITGDRNE
                     ADEALVEVWTRAWRESARYDLNRGTVAAWLLGIARSCALSRVPAGAVSGRRGAGDFDA
                     GAALADDPDDSPGRRQLAERVRRALGALEPKHRRVLESSFFDGLSTLEIAGRMNAPAV
                     MVRSWTRQALARLRALLPSEEWT"
     gene            15931..17361
                     /locus_tag="VFM00_06560"
     CDS             15931..17361
                     /locus_tag="VFM00_06560"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7497943.1"
                     /translation="MAGKRLLILGGGFAGLDVARAIGRSRAAREYWDTLLIDKENFFQ
                     FNPLLPAVAVGSVETRHIVYPLREMARHRHIRFHKNKAVRIDLDTRQVVLHNSLAEPY
                     DTLVIAMGSVTNYYGVPGAEQYTRPFKTIADAMTLRARVVELFEMAEQAETLEKRRRL
                     LSFALVGGGVTGVEVAAELIEMARETLLPRYPSIRAADISVTIVEGGDRVVPTARPEH
                     SRYVHRFLERRGVKVLLHTPVRRVEPYRVLAGEDRRVDAFTIIWTAGVHPPDLVRDLP
                     VRHHRDGRVLVDDRLHPTREDGTPLPDVYVLGDCAASPRPDGTMQPALSQTGIAMGAF
                     TGRQLVRVAEGKPFERFAFKDAGYIISLGKHSSVLELFGIPLSGKIAWLLWAGAYLVK
                     MVGFRKQVEVGIDHLSHLFFEHDTSQILARRAVLSDDELNLSLAEPGRDGSEAPGPGA
                     ASEGGSQASNPRGELVTTPGETRRRT"
     gene            17435..17833
                     /locus_tag="VFM00_06565"
     CDS             17435..17833
                     /locus_tag="VFM00_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497944.1"
                     /translation="MKRTTLILTVAIAAACLAFALGTALAADVHAGGKPTTLSGELVD
                     MGCYIGHMAKGEKHADCAAKCVSGGMPMGVLTSSGKLYLLTMDHANPDAYNKAKDFAG
                     KQVKITGPTHVRNGVNTIDVVSAETVAMAK"
     gene            18002..18433
                     /locus_tag="VFM00_06570"
     CDS             18002..18433
                     /locus_tag="VFM00_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497945.1"
                     /translation="MKPLPRKHLIAVAALLTLVAAAPPPAPKGPKKQVTVTGEILDMG
                     CFISRGLRDELHRECATKCLAMGVPMGLLGADSTVYILTQNHDRAMEPSAFQPPDPFA
                     QCKGWAAKQVAVTGFVWERKGYKEIEVKTARLAPAPSPKVP"
     gene            18430..19491
                     /locus_tag="VFM00_06575"
     CDS             18430..19491
                     /locus_tag="VFM00_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497946.1"
                     /translation="MSLSTRGRIPLSTLPASWRRLLVGGAALLLVTVAPIARAQETSQ
                     QCLDHSKEAAAEAKKAMAASKGKTTLAKAPAVAAAPAPRGAAPANAGAPAAAKANPAG
                     KPMKRTLTTLRGELVDYYCYIEKGARGPEHKECALRCVAGDICMGLLTPDDQLMMLSV
                     QHLRVMEPLAWRGIPDPFTQCRGLLNETVDLTGYVMQRKGQKIIEVTAVKVVKAPPNP
                     KSAAYKFTIPTPPKPGPNVVQHHEKRVPTTLRGELVDYYCYIEKGARGPEHRDCAVRC
                     VAGDVCMGLLTKDEKTLMMLSVNHLRAMDPRAWRGVPDPFTTCRSLLAETVDLTGYII
                     ERHGQKIIEVTDVKVVPKS"
     gene            complement(19530..20300)
                     /locus_tag="VFM00_06580"
     CDS             complement(19530..20300)
                     /locus_tag="VFM00_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7497947.1"
                     /translation="MSDTRDEVKRRFGASAENYVRSKDHAQGESLDRLIEVTAPRSEW
                     RALDVATGGGHTALALSPLVREVVATDLTAPMLAAAERFIREKGVANVRFQEADAGAL
                     PFEDASFDLATCRIAAHHFPDIAAFVREAARVVRPGGLVAVIDNVVPEDPEAAAAINA
                     WEKARDPSHDRALPASEWRQLFQRAGLAIEHDEAFRKARDFEAWIGRMPVDAATKERV
                     RAMLTDSRGGMREFLRPEGEDGALRFYLEEILVIGRRS"
     gene            complement(20297..20911)
                     /locus_tag="VFM00_06585"
     CDS             complement(20297..20911)
                     /locus_tag="VFM00_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7497948.1"
                     /translation="MPNVMGVLRAEIRRLARKETKEAVRDLKRQVAALRRRMAQAKRQ
                     LAAVERAAMRAASGRAAAAPAAAASEEDGTQIRFSPRWVKVHRSRLGMSRRVYAKLVG
                     VSPQTILLWESGKARPRRSALASWRAIRGKGIRELKAAAGGETRGRRGRRAAAKSEAP
                     ATGRKPRGKKARGGGRRKARRAGRTVRATKKAVRTPRRARAKKK"
     gene            complement(20937..23135)
                     /locus_tag="VFM00_06590"
     CDS             complement(20937..23135)
                     /locus_tag="VFM00_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET7497949.1"
                     /translation="MNAADTPGSFGPFRLEARASGVAVLWFDDPARKVNLLDSNALAS
                     LRRALDALKKRADASFPRALVLTSAKEGNFIAGADVNEFDTIASTADAEAKVKEAQTL
                     FQEWSELPFPTVAAINGACLGGGTELALAMRYRIAADKGVTIGLPEVQLGILPGFGGT
                     TRLPRLVGLIPAMSLLLTGRSLDPRRARKMGLLDDVLPAARFSERAVAWTEALLSSPS
                     PPRRAAPALPNRIMQSVPPFRSYALGQARQTVLRETKGHYPAPLEIVRVVGASWGKPI
                     LEALKIERKAVAKLLFTPESESLRRIFQMNERAKRVPPADKARRVETAAVLGAGTMGG
                     EIAYLMSMRDMRVRLRDIKPEPILKSLAHARSLFKREVSRYRLTRSGMEMAMGRIEPT
                     LDLQGFRRVDLVLEAVVEDLNIKQALFRELETQVGPACVFATNTSSLSVSAMARGLKH
                     PERVVGMHFFNPATRMPLVEVIRADDTDAASLDTVVALTRRLKKTPVIVADKPGFLVN
                     RILMPYLAEAVGFVERGQSIPAIDKAARDFGMPMGPLELLDEIGMDIARKVAHVLLDA
                     FGDRIPSLALIDKMVSEGALGKKANLGFYRYERGRRSGVNPALASLATSREPVPAAEI
                     ADRMVDAMVNEAALALDDRVVEDPADVDLSMILGTGFPPFRGGLLRHADAVGIGTIVE
                     RLARRQQAGAPYGPCGRLQRMALAGERFHEPRPQSRTGQPPSEAAAAGRR"
     gene            complement(23132..24418)
                     /locus_tag="VFM00_06595"
     CDS             complement(23132..24418)
                     /locus_tag="VFM00_06595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET7497950.1"
                     /translation="MRDVVLIDGFRTPFAKSGTALAKVHARELGRVAIAELLARHGVP
                     PSEVDEVILGNVAQPSDSANIARVIALLAGIPERVPAVTVHRNCASGMESLVQAHERI
                     AAGNADLIVAGGTENMSQIPLIVSEGMTRAFERSSRAKSLGQRIGAFAGIKPKDFAPR
                     VAIMEGLTDPISGLNMGETAEVIAKDFGISREAQDDFALMSHRRASAATESGRLAEEI
                     VPVFAPPYEQAVIEDVGPRKNQSLEALAKLRPFFDRRYGTVTPGNSCPITDGAAAMIV
                     ASAEKARELGLTPAGRIKSYAFVGLDPVRMGLGPAYAIPPALERAGARFTDVGLIEIN
                     EAFAAQVIGCETAMASARWCREELGLERPVGEIDRAILNVNGGAIALGHPVGVSGTRL
                     VLTLLREMAHRNVPLGLAALCVGGGQGAALVLERAA"
     gene            24505..26181
                     /locus_tag="VFM00_06600"
     CDS             24505..26181
                     /locus_tag="VFM00_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET7497951.1"
                     /translation="MDVQTIPELLRHARETHRKPNAFLVRRAGVWVPVSIETYAAQAA
                     SAGAWLRAQGVRPGDRVAILSESRYEWVVADMAIVSMGAVSVPFYPTLPAGQIAPLVA
                     DSGIVGAFVSSNEQEEKLKLVQSERAGSSEALRFVWNFDASGLPEAPAGAGDVPLPRP
                     DDLATIIYTSGTTGTPKGVMLTHSNIVSEVVISLTTWKLNQDDVYLSFLPLSHVLERA
                     SGFFTMLYAGVTIAYAESFDKLPRDLREVRPTVLIAVPRLYEKVIARAVETAAGAGQF
                     TRSIFQNAYRVAVEVGRLKNEGKAVPPWTILERAIGFALVYGKIARGLGGRTRLRVSG
                     GAPLNRDVALFYRGAGLAVHEGYGLTETSSAISVNTSERQRVGTVGPLFPGIEVRTAE
                     DGELLVRGPVVMKGYWQKPEDTREALEGGWLHTGDIGALDADGFLRITDRKKDLLVTS
                     VGKKVAPQPIEAALKASPRITEALVLGDGQKFVAALIVPAAGATREAIAEDVDRVNGT
                     LAQFERIKRFELIPDDMTVEKGLMTPSLKLKRKAVVEHHREAVEKLFRGA"
BASE COUNT         4078 a         9142 c         9259 g         3818 t
ORIGIN      
        1 ccccacgcga tccgcgcggc ttcttccgcg tcgtgcgcca cgcggagcag cccgcgcagg
       61 tcggggccga actcgaagcg gcgatcgagg aagtcgagga gctcgtccca gccttcgccc
      121 aggagcacga gcgcgtcggg atcctcgtcg cggtcatgcg tgagcagcgt ccagacccag
      181 aagagctcgt tcagcgttcc cacgccgccc ggcaggacga ccgcggcacg cgcgcccgcg
      241 agcagattcc ggatccgctc gtagaggtcg gccgcttcga cgacctcgct cagctccgag
      301 gagggcacgc gcgtctcctg gtaccaggcc agcgtgcagc cgacgacctt gccgcccgcg
      361 gacgtggcgc cctcggtcac cgcctccatc accccgccgt acccgccgca gcgcacgacc
      421 gcgccgcgcc gcgccagggc ggcgcccagc tcgcgcgcca gccggtggtg gggcgaatcc
      481 cgcgctgcct tgctgctgcc gaagaccgcg accgaagtct ttgtcatcgt gccgaatata
      541 ccacctgaag ggtgcctgac cgaccggggc ggccgagggg ccggaccggt cacaaaccgc
      601 gcgcgtcatg gtagcatcgg cccatgaccc ctcaacgcgc cgcgcgggcg cacacgtccc
      661 gatcccgcgc gagccggatc gtccgcatcg cgctcgccca ggtcaatccc acggtcggga
      721 atctccccgg caacacgcac aaggtcatcg agttcatcgg ccgagcgcgg gagatgggcg
      781 ccgagctggt cgccttcccc gagctggtgc tgaccggcta cccgcccgaa gacctcctgc
      841 tccgccccga gttcatcgac cagaacatgg cggcgctgga tgaggtgacg cgcgcgacgc
      901 gcgggatcac ggcggtcgtc ggcttcgcgc agcgcgccga ggacgtctac aacgccgcgg
      961 cgatcgcgca cgacggcgag atcgcgggca tctaccacaa gagccatctc cccaactact
     1021 cggtcttcga cgaggtgcgc tacttccgcc cggggcggaa ccccttcgtc tgcaggcgcg
     1081 gcgcgctcac gttcggcgtg aacatctgcg aggacatctg gctgccctcc agcctgaacc
     1141 agctggccgt ctacggcgac gccgacctgg tgatcaacct ctcctcctcg ccctatcaca
     1201 tgggcaagtc gatcgcgcgc gaccgcatgc tcgccacgcg cgcgcaggac gcggtctcgg
     1261 tgctcgcctt cgtgaacacc gtgggcgggc aggacgagct ggtcttcgac ggaaacagcc
     1321 gcgtcttcgg cccgcggggg acgacgctcg ccgcggcgaa ggcgttcgag gaggacctcg
     1381 tcgtgctcga catcgatctc gacgaggtct tctcggcccg gctcaaggat ccccggagcc
     1441 gcgagctggc ccaccgcgac gagccggagg agccgatccc ggtcgtggag ctgcgccccg
     1501 tgcccgaccc gccgcggccg ccgatccagg cgcgcgccac ggtgacgccg gcgggagagg
     1561 ccgaggtcta cgaggcgctg atcctgggga cgcgggacta cgtgcggaag aacggcttcg
     1621 agcacgtggt gctggggcta tcgggtggga tcgattccgc gctcaccgct tcggtggccg
     1681 tggacgcgct gggtgctaag aacgtgaccg gcgcctacat gccctcgggc ttcagcgcga
     1741 aggagagcga ggccgacgcg acggcgctcg cgaagaacct gggcttcccg atgctgacgc
     1801 tgccgatcgc cgccgagatg gaggcctacc ggaagtcgct cgcgccggtg ttcgagggac
     1861 gcccggagga cgtgacggag gagaacatcc agtcgcgcat ccggggcaac ttcctgatgg
     1921 cgctctcgaa caagttcggc tggctggtgc tcaccacggg caacaagagc gagtacgcca
     1981 ccggctacac gacgctctac ggggacatgg ccggcgggtt cgcggtgttg aaggacgtgc
     2041 cgaagaccct ggtctacgcg ctcgcgcgcc atcggaacgc gcgcgccgga aagggagcat
     2101 ggattccgga gcgcacgatc gagcgcccac ccaccgcgga gctgcgcgcg gggcagctcg
     2161 acaccgattc cctgcctccc tacgacgtgc tcgatccgat cctgcgcgcc tacgtcgagg
     2221 aggactggga ggtggatcgc atcgtgggga tgggctacga ccgcgcgctg gtacagcgca
     2281 tcatccgcat ggtggacaag gccgagtaca agcgccgcca ggccccgcca ggaatccgca
     2341 tcacgccgcg cgcgctgggc aaggatcgca ggcttccgat taccaacggg catcagagct
     2401 ggcgggactg aacggcgggg ccgagcagac tcaccgaagc acacgcgcca atcgcaggtg
     2461 agaatcacga cagctgtcgc gggattcaca ctcgccagcg gcggtgaaat catggacagc
     2521 tgtcgccaga ttcacacgcg ccagcgcggg ggagatcgaa gacagatgtc gccacgttca
     2581 cacgcgccca gcgcgggtga aagcgaagat cgatgtcgct gagttcacac gcgctcggcg
     2641 cgggtgattc ggacgtcaaa ggtcgcagag ttcgcgacct atcgccccgc cgtagcctcg
     2701 ttgttcagcc aagccctcgc caggtcgatg tcttcctgcc ccagtgcgtg gcccgtatca
     2761 ctccactgca acgtcacgtc ggcgcccgct ccgcgtaaga gctcggccca cttctcgctc
     2821 gcgtagggac gcaccaacgg atcgttgcga ccggcgccca ggtagacgcg ctttcccgta
     2881 agcgcgggcg gcacttccgg cgtgaacggc gtcatgcccc gcaggagaat cgcccgctcg
     2941 agcagatcgg gatggagcag cagcagcgcg cccgcgatgt tcgccccatt cgagaatccc
     3001 accgcgacca tcggttgcgc ggcgattccg tagcggtcgc gggcggcatg gacgaactgc
     3061 gccagctcgt cggcgcggtg cttcaggtct tcctcgtcga agacgccttc ggcgatccgg
     3121 cggaagaacc gcggcgctcc gttctccaat acccggccgc gcggcgagag gatagccgcg
     3181 ccgggcgcga gataggggcc gagcgggagc atgtcgtcct cgtctcctcc cgtgccgtgg
     3241 aggagaagga ggaggagcgg cgatccgttc ggctcgggtg gcacgaagcg gtgatgaaag
     3301 agcgacagcg ggtcgacggc gtgcatgggt tcgagcggcc tgtggtcgag gttcggggtt
     3361 gtggaaccga acgggcgtga tccctcggtc cagggtcggg gcgattcgac gcggcgaacg
     3421 gccatcaccg cccgtcgcgc ttcgcgtgaa gccgacgcgg cgaacggcta gcggagaatt
     3481 gtaatcgatg cggcgggcgt gcgcacgttt ccggggacga gcaaccgcgc ccagtaccgt
     3541 cccgaggcgg cgggacggcc ccggtcgtcg cggccgtccc agcggacgcg ggtcgcgtcg
     3601 ccctcggcgc gcagcgtgcg gaccaggcgg ccgcgcacgt cggtgatcgc gatgcggatc
     3661 gcaccgccgg tcgagccggc cccgccggcc gagccaaccg agccagccgc acccgcaccg
     3721 cctgaccgcg cctccgcgag cgcgacggcg cttcgcgacg cctccagcca gacggcgccc
     3781 gaagaaggat tcggcgcgac acggagcgcg ggaacggccg gcgccggcga ggccgccacc
     3841 gcgacgcccg agatcgcggc cgtgccgcta cgcagcacga gaagccgcgc gccgtcgctc
     3901 acgaccagat cgggcaggaa gcccggcatg tcgcggaacc ggagcgacgt ggaggacgtg
     3961 gcccagctca cgtgcagggc gcgctcgagc cctcccgacg caccccagga ttgcactcgg
     4021 gagagactga ccggctgcgt gtagggcgcc ggcgcgcccg gctggatctt gtccagctcc
     4081 gaggctcccg gaacgcgaag caacgagacg ccgccgccct ccatcgcgac gccaagcgtc
     4141 cccgtccacg cgctggcgag ccccaccgcc ggcgccacgg tctgctggac gagcacgagc
     4201 gccgggggct gtcccccgcg aggagcgcgc acgagctgca ccccacccgc gcccgtggcc
     4261 acggccacgt aggcgctgtc gccggtgttg gccacccagg ccagatcgcg cgcgagcgcc
     4321 gagccccaca tcccggagag ggcgacgttc ccccgcggcg tgaaggtgac ggacgccgga
     4381 gcgcccggag cgaacgtcag tccatagaac gccaccccgc cgcttccgtc cgcgagcgcc
     4441 atctcgggag ccgagcctcc tccgcgcagc tccaggtcga gcgcgctccc cggcgtcggc
     4501 agggtccccg ccgcgtaagg cacgccgccc agcgagtcga tggccgcctc gaggtccacg
     4561 gccaccacgc ccgcgccggt tcccgagatc accagggcgg ggtcgtagga cgccgcgacg
     4621 ggaatggacc cggtgtcggc atcggcgacc agcccgacgt ggcgtcccgt cgccgtgcgc
     4681 acccaggcgg cgtcggtcgc gaagccagaa gtcgggatca cgcgcaggat ccggtcgtcg
     4741 accccgtttc ggtccacgtc gaagaagtcg ctccagtccc cgaagagcgg gtcttcgatg
     4801 gtgcgcaggt cgagcagcat caacccgccg cgccccgcgg ccagcaggcc gaggtccagg
     4861 gtgtccccgc tggtggtgac gaatccggga aggatcgaga gcgcggccgc gtacgaggga
     4921 atccggatcg tgccgacgac gcggtcgtcg ccgccgtcgc cgttgccgtc gatcatcccg
     4981 atcgagtccg tcgccgtcgt gcggatcacc cgcacgccgt cctctccgga ggggaccagc
     5041 cagtagccgc gcgaggccag cagcgcggct ccgtcccgcc cggcggcggc ggccgacaac
     5101 ggggtgagcc ccatcgggac gagggcggtc ggaagcgaat cgggcagcga agcgctggac
     5161 aaggcgaagc ctgacgcgtt ggagactccg cccgagttgg tgacgctgag ctggaccgcg
     5221 ttcccgttgc ccgcgaagga gcgcgcggga atcgtggtgg gcacgttcac cgtaatggtg
     5281 cccccgcccg gaagcaaagc gctggcggga ggcgggctgc ccgcgaacag gccgcccgcg
     5341 ttgttggggt cgggattgaa ccgcccgccc gtcaagagga ctgtctcgct cgtgccggtc
     5401 atggagtgga cctggaacga gaccgaacca gacgtgcccg acgcggccga ccaggtcgag
     5461 ggcgagggcc acacttcggg acggccgtcg ctggcggcag tgatcgaggc gccgaccttg
     5521 gccagcctcg cgccgccggg atcggcgagg atgtccaccg agatcgacga gctccccacc
     5581 gccagcgccg gcaccgtgac gttgatcgtc accgaggcga tgctgttcgc gggaacggac
     5641 agcacgccgc tcgatggcga gagggtcgcg acgaacgcgc cgccgctcgt cgccgcccag
     5701 gcgacgttcg cgagcgatcc gttcgcgcgc agcgtcaggg tgagggggcg cgtgaccccg
     5761 ggagcgatca cggcgctcgg aaggagcgcg gtgatccgcg ccggcgggat ccaatcggtc
     5821 gctcccgccg gacgcgggac cccagccagg gcgagcgtga cggccgcgca ggaaatcgcc
     5881 cgcgcggccg cgcgcgcggc cctacgaaga agggggccgg cgatcggcgc cggccccccg
     5941 gtgcagcgac gaatcacgag actgccgttt ccttcacgcg atcacttgag aatcgtgacg
     6001 cgcgcgtcct gatgccacga ccccgactcc atccgggcga agtacgtgcc cgaggcgacg
     6061 gtgcggccgg ccacatcccg cccatcccac agcgtcgtgt gcggcccggc ttccatcacc
     6121 ttgccgtcca ccagggtccg cacgaggcgg cccgttacat cgaagatgtt caggcggacc
     6181 gccgcattcc cggcgttccg cggaatcacg aagaggaact tcgcgaccgg gttggccggg
     6241 ttggggccgc tgttggcgaa gaagaactga tcgggatcga tggtccggcc cgcgatccgc
     6301 gccgccgcgg caggcggcgt cgtgaccgaa gcgctcaaca cgtacgtctg cggcacgcgc
     6361 acctgcggca ccggattcgt gagcgtcgag ctgcggaagt cggtcacctg gacggtgaac
     6421 ttccgcgcgt tcttcgaatt ccccgggttc ggcggcgccg tgaagacgac gcgcgggttc
     6481 agccccgtga agtcgtcgtt gaacgcgacc acgtcgccgt tcgaatcgta gaggaacatg
     6541 accaggtcgg tgttgttgcg gccgtcctgg cccgtcgccg tggcgctcgc gtcgatgatg
     6601 tcacccggct tggccaggac cgagtagaag tcctgatcgc cggcgggcga gtaggtctcg
     6661 gcccggttgg tgacgttccc cggaagcacc ttggcctcgt tcaagcggcc gttcggctcg
     6721 tccgcgtcct cgttcagggc gaggatcgtg accgccgact ggaggctgcg gtgtccgttc
     6781 ttgtcgagcg actgggacga ccagatcgtc gctccacccg tgctcggcgg cgacggcgag
     6841 aggtggaacg cggccgcgcc cgcgccggcc gtgtcgcggt cggcgtaggc gaagggactg
     6901 cgctgaatca ggaaggtctg atccaccgaa ttctgcggca ggtcgaagcc gtcggcgcgc
     6961 ctgttggccg atccgacgta ggtgaactcg gtctgcgtgg cgcggcggtt ggcgccgagg
     7021 ccggtcgggg ccacgatcgg caccgcgttc ggatcgggaa gctggcacgt catgtcgacc
     7081 gcgagattgc ggtcgatcag cacgcccgac gccccgctga gctggaccga gtacgagttg
     7141 gcgaacagcc ccttctccag cgtcgtgaaa tcggcgcgga tgaacgggaa gttgttcggg
     7201 aagcccggcg tcgccgcctg gctcgggaac cggatcaccc agaagaaggt ctggttcggg
     7261 gtgttcaccg gcatgttgag cggaacgacg ttgtttccgt tcggattccc cgcgtagagg
     7321 gtctggacga agagcgggtt ggcgagatca ggaccgccgg ttaccaggct cattgggcag
     7381 agcagaatct gcgggaagag cgccgggacg ccgttgaacg tgaagctcgg gaagctgacg
     7441 ttcgcgatcg tgtagggacc ctcgaacgga ggatcgaagc gaaccgcgag ataggcgtta
     7501 ttcgcgaacg tgacgatgtt ctcgaaggcg ccgtgctgcg cgatgatcac gcgacccggg
     7561 aaggtgttcg agatgaagtt cacgcccggg gtattcgtac cggcgttcac cgtcaccgga
     7621 tcgaagctgt tcgcgaggtc ggtgaagccg ttcccgctct cattggcgcc gttccagaac
     7681 tccggctcga agccgatgtc gaggccgttg tacggaccgc cgatgttggc gtcggccgcg
     7741 atggtgttca ccccgtcgag cggcacgatc cgcacggcgt aggagcccgg aggcaggccg
     7801 tgaatcgtgt actcgccctg gccgttccgg agcgtcagct cacccgagat ggcactgatc
     7861 gagtgggccg cgtccggcac cgcgaggttg taggcctcga cgtgcgcgcc gaagagggcc
     7921 aggttgttga agccgttcag gaccgtgccg ctgatcgtcc cggtcgtggc gttgaagtcc
     7981 acgccgtcgg ccgagagtcc gcgatccgcc gactccggaa agatcgtgga gattccggcc
     8041 cggtcgtcca gctccagggt gcgctggctg atgtcgttcg agatgaacgg caccatggtc
     8101 gcgcccgcga cgctggtgtg atcgaggccg aagtagtgcc cgtattcgtg ggtcacgacc
     8161 gcctggagat cgacgctcgt ctgcccggcc ggagccggcg tcagacccgg cgaggagatc
     8221 gaggagaaga actcgtggtc gttgaacacg aggtccgcgt ccagcgtgtg gccgttggaa
     8281 aggtcgacca cggagcggac gaacgcgatg acgccgctgc cgaccgcgaa attcgcgccc
     8341 ggcgtgtcga agaacatgct gttctgcccg tcgaacgcga gcttgggaag cgaatcggtc
     8401 gccccgaact cgtgcgggtt caccgtgaag tacgaggtgt tgacgttctc ccagttcttc
     8461 gccgactgga tcaccgtcgc cagggcgtcg gtcgaggaga tgtcggcgtt cgcgggattg
     8521 atgacgctgt tcagcggaag ctcggaatcg aaccagtgcg cctggacgac gggcgaggta
     8581 gcgtccgcct ggcgcgccag acggatgaac gccgcggccg gattcgccag cgtgagcgcg
     8641 agcgctccga agacgagcac ggcggcggcg atgcgaaatc gaatcgaatt catggtcgtc
     8701 gctcctccgt tactcgttgg ggaccgtggg gtcgatctgg atcttgccgg cttcgtcgag
     8761 attggtgcgg acgccgtcga gcagcgcctg gagcggcatg tcggccgtct tgccaggctc
     8821 gaacgccttg ccggacgcgt cgatcagcga cgtgccttcc agctcgttgc ggacgcgctt
     8881 ggttccgttc tgatcctgcg acaccgtgta cttgccctgc tggagtccca tgatccaggg
     8941 atacccggcc ttgtcgttct gcgacagaaa tacgacgacc tcctccccct tcttgaaggt
     9001 cggcatgccg gggacgatcg acgcgatgtt gtccaccacg ccgcccagct ggcggatggt
     9061 gatcacgtcg tccttgcggg atcccttcac cgggtcgagc acgcgaaggg tgacgtaggt
     9121 gtagatctcc ttgttcgcgt catagcgggc gacctggtcc tcgacgcgcg cgacgaccac
     9181 ggcgttggac ttgagggcca gatccttgag ggacatcttg gtgacggtgg tggcgtgcgc
     9241 ggtgctcgtg cccacgaggg cgagagcggc gaacgcgacc atggccaaac ggcttgcgcg
     9301 attcatcgct tgtctcctgc ggtgactcct tgcggcgccg ggacggatgc gatccccgcg
     9361 cgtcgcgtga gatgaagcgc atgggaactg cggacggaac aatgcgggta acggtgtctg
     9421 tgccggctct caccccccta gcatccggcc caggcgggcc ggcatcggct gcgaaccccg
     9481 ttgcggaggc ggaggctccc gggcggcagg gcgccgcgcg tggaagcgcg ccgaatattc
     9541 gacgagaagg cgtaattata ccattttgaa tgctttatgt caacagggtg atttggcccg
     9601 acgccgccgt cagccccctg aggagcttag aggggccagg ccgcgattcg gatggaacgc
     9661 cctcccgaag ggtcggacag ggagaagtcg gcggtcccat cggcgtccag ccggtcggtc
     9721 gacaggtagc ccatcccaag gccctcgccg gtgccgggct cgggcgccgc gctggtgagg
     9781 aatccggccg gcgaaccccc ggccaggatc ggagtcccct tggcgggggc cgagcccggc
     9841 cagggaaagc ggaagccggc gcggcggcgg cggacctggc cgcggttcga cgttcgcgcc
     9901 acgacctcct gcccgacgta gcaccccttg gtgaaggaga tcgcgccggg caggtcgagc
     9961 tcggtggcga tgcggtccag gtcgaagtcg gcgcccgcga cggggattcc cgccgcgatc
    10021 cgccacgcgt ccgccgcctc gcgcgcggcg aaggccggca gcgccgtagc ggcgggcaga
    10081 aacaggaacg aaagcggcgc accggcaata gccgtcgcgc ccgccgcccg ccccgccagc
    10141 gaagccagga gaccgctccg gttcggaccg gcgacgccga cgaggcgcgg cgttgtggcc
    10201 ggaccctcga attccacatc ctccatgatc cggaaccgct ccaggtgcgg gcgcagtgct
    10261 tcggccgagg cagcgtccgc caggacccac gcgtcggcac ccgaggccgc cacggcgacc
    10321 catgcgtgcg cgaccggacg tcccttctcc gtcaggaaca gggtctcggc cgcgcgctcg
    10381 gggcccgcgg cgtcgaggtc ctgggacgcg gtgccttgga ggaactcgcg cgtgtcggcg
    10441 ccggagagcc gaaggacgcg ccagtcgccc aggtccagcg cggccgcatc ctctcggagg
    10501 gctcggtaat cgtcggcggg ggatggcatg gcacgaagaa tagcaccccc gaaagaaggt
    10561 ccttgacgcg ggcgccacta aatcgtatat ttacgattaa cgatatgcga gaggaggccc
    10621 gatgaagtcc ataacactca cgccggccgg aaggcgcccg ttgccccgct cgcgccgcac
    10681 gggagctccc ctggacgtgg agctggcgcg cctggcgaaa gcgctgggcc acccggcccg
    10741 ggtcgccatc gtgcggctgt tgctgggaaa gaaggagtgc gtctgcggcg acatcgtcga
    10801 ccggctgccg ctcgcccagg ccacggtgtc gcagcacctc aaggttctca aggatgcggg
    10861 ctggattcag ggaagcatcg acggtccgcg cgtctgctac tgcgcccgtc ccgagacggg
    10921 ccgcaaattc ctggagctgg cgcaagcgct ggcggcccgg gaggaggaag gagaaacgtc
    10981 atgagcgcca cgaacgacat tcgcgacacg gtgcgggaac gctacggagc gctggccgag
    11041 ggagccgact gcggctgcgg atgctcctcg ggaacggtct cctgctgctc caccgattcc
    11101 caggccatgg ccacgctggg ctacaccgcc gaacagctcg ccgcaatccc ggagggcgcg
    11161 aacctcggac tgggatgcgg caatccgctg gcgcacgccg ccgtgaagcc gggcgagacg
    11221 gtgctcgatc tgggatcggg tgccggcatc gacgccttcc tcgccgcgcg cgaagtaggg
    11281 gcggagggcc gcgtgatcgg cgtcgacatg acgccctcca tgatctcccg cgcgcgggag
    11341 aacgcggcga agggcgacta tcggaacgtg gagttccgcc tgggcgagat cgagaacctg
    11401 cccgtggccg acggctcggt ggacgtgatc atctcgaact gcgtcatcaa cctgtccccc
    11461 gacaagccgc gcgtcttcgc cgaggcgttc cgcgcgctca agccgggcgg ccgtctcgtc
    11521 gtgagcgacc tcgtgctgac gcggccgatt cccgacgcgg tccggaaatc gatcgaggcc
    11581 tacgtcgggt gcatcgccgg cgcctcctcg cgcgaggact acctccggct ggtgcgggag
    11641 ggcggattcg acgacgtgac cgtgctcgag gagcggggct acggacagga cggccctctc
    11701 ccgaccgtgg tgatcgaaga gagggcctgg gacgccgtcg cgtccgtgaa ggtgcgtggg
    11761 gtgaagccgc cgaaccgtac ctagctacgc ggtggtcttg tgcaggacgg cgtgctggcc
    11821 cgcgtccccc tcgccgcgcg agcggatcca gcgctccagg cgctcgggcg tgccgagatc
    11881 gacccagccg cacggaggca cggcgaggac gcgtagatgg gcggcggagc cctggagcac
    11941 gtcgcgcgag aagtcgacgt ggggcagcgc ctcataggcg cgctccaggg ccgcgcgatc
    12001 gggcaccgct ccgggctccc gctcttcggt gacgcgggcc agtgacgcca gcaacgccgg
    12061 cgccgcggtc tgaaagagcg cgaggagcgc gcgtccgcgg gccacgacga tgaagctgct
    12121 gactgacgcg ccggagcggt acagctcccg cgccatgcgc ggggtcggct tttcgacgaa
    12181 ggagacgatc gggcgggtcc cgtcctcttc cgcctcgcct ccgacgatcc atccgtaatc
    12241 gggatcggga ccttgggggg ccatgcccag gaccacgacg cggcggccgc gctccacgac
    12301 ggagacggcg cgcgcgagcg aggacgccag cgtcgactcg tcggcgacgt actgatcggc
    12361 gggaagaacg accaccaccg cgtcgcggct cttgcggagg acgcgcagca gcgggagcag
    12421 gatcccagcc ccggtgcccc ggttcgccgg ctgcaccatc atgccgggga tcatcgagcc
    12481 cgggaccagg tccccccacc attcgcgatg gtgcgcggcg acgacggtga cgatgtgagc
    12541 gggccgggtg agccgcttgg cccgatcgaa cgcccatcga agcatcggcc ccgcgtctcc
    12601 gggcgcccag tattgcttgg gaagcggccg tccacggcca tccatggtca ggcggcggac
    12661 gcgttctcca tcaccggcag ccaggatgat cgcccatcga cgcgaggcca tattccgcga
    12721 caccccctac accacgaagt tgacgctcgg ggagaatgca gaagccatgc cgcaaggggt
    12781 gggcccatgg cgtgggacgg aagcggggtg cggcaacggg cttagagtgt gtcgcggtcg
    12841 ctccgcgcgc gcgctgcgcg cgcgcggacg actgccagac tgtcagggaa aacgggccgt
    12901 tatggcaggg attcgcgcac ggaagcgcgt ccgtccgcgt cgtaggcgat gacgctgtgc
    12961 acggggatcg cgcggtcgat cttggaccgg ccgtcgagga aggagagctc gacgagaaac
    13021 gcgaagcccg ccggctcgca gcggatcgcc cgcaccaggt cgagcgcggc gcgcgcggtg
    13081 ccgcccgtgg ccatcacgtc gtcggcgatc acgacgcgcg tgcccgcggg caccaggtcg
    13141 cgatgggtct ccaggcaggc gcggccgtac tcgaggtcgt attcctcgcg atgggccgag
    13201 gccgggagct tcccttcctt gcgcgcgagc agaagcccgg cgttccagcg cgcggcgagg
    13261 gcggcggcga tcaggaaccc gcgcgcttcg atcccgagca ccatcgcggg aggcgccgca
    13321 tcgaacggcg ccgccagcgc tcgcaccgcc cgcgcccatg cgtgaccgtc ggcccagagc
    13381 ggcgtcaggt cgcggaagag gatgcccttc ttcggccagt cgggcacgtc gcgcacccag
    13441 gcggtgagcg gcgcctcggc ggtatcgggc ggagtccagt tcacgggcgc caacgtagcg
    13501 ccccagtgcc cgcgcggcaa cacctacaat cggcggcatg cacgtcctgg tctcgggctc
    13561 gagcggattc ctgggaagcg cgctgatgcc gcgcctggcc gcggccgggc atcgcgtgac
    13621 gcggctcgtt cgatcgcgcg gcggcactga aggcggcgcg agcggtcgcg gcatggcgga
    13681 ggtccgcgcg tgggcgcccg acgccgacgc gctggacccg cgcgtgctcg aggggatcga
    13741 cgccgtgatc cacctgggcg gagccagcat cggctcggtc tggacgagcc ggcggaagga
    13801 gctgctccgg accagccggg tccgaacgac gcgcctactg gcggagcgga tcgcctcggc
    13861 gtcgccgcgc cccgccgtct tcgcgcacgc ctcggccgtc ggctactacg gagaccgggg
    13921 cgacgaagtt ctcaccgagg ccagcgcgcc cgggcggggc ttcctggccg atctctgccg
    13981 cgactgggaa gcggcctcga tgccggcgca ggacgccggc gcgcgcgtcg tccgcgtccg
    14041 cgccgggctg gtgctgagcc cgcgcggagg cgtcctgccg gtgatgctgc gccccttccg
    14101 gctcggcctg ggaggacgcc tgggcagcgg acgccagtgg atgccctgga tcgcgctgga
    14161 ggatgcggtc gccgtctacc tgcgcgcgct ggaggacggg gccctggtag gggctgtgaa
    14221 cgcggtcgct cccgaggcgg tcaccaacgc cgagttcacg cggtcgctcg cgcgcgccgt
    14281 cagacggccc tccttcgtca cggtgccggc gttcgctcta gggctgctgc cgggcgggat
    14341 gggaaaggag acgctgctcg cgagcgagcg cgtcgttccc aaagcactcc agaccgcggg
    14401 attccggttc gaatccccta ccctcgagac ggccctctcg gcgattcgcg gagggggccc
    14461 atgagcacga ccggcccgct cggggccgtc ttatcgggcg agccgccctt cggctccagc
    14521 gagaccgcga acgcggtcag cgtggtcggc tcgccggcgc tctccagccg gagcaccgca
    14581 gcgccgtgct cgtccgggtg gatcaccccc atgctcgtgg tcgagagtcc gcggaccgcc
    14641 cagagctgaa agctctggct gtccggcgcg gtggcgttcg tgagcacgac cacggcccgg
    14701 cgcgtggcgg gatcgtagac cgcgcgcccg cggatcccgg aggtctcccc cgcgagcggt
    14761 gccgccggcg ccagatcggc gacgcgcgcg atcggcgagg tgagcacgcc gccccactgc
    14821 cgctccaccg cgaggtcgcg cgcggtgccg ttcaggtccg cctcgcgcgc gcggagaatg
    14881 gcgcgaagcc gaccggtcga ttcgtagaag gtgcccgagg agatgatcat gaccgcggcc
    14941 acgatccagc ccgtccaggc gaaccacccg gggcggccgg tgcgcgggag gcgcaggacc
    15001 ttggcggcgc ccggtgcgcc ggagccgccg gggacggccg cgccgcgatc gcgcgtgcca
    15061 tgctcggccg cgccctgctc ggaggtcacc gcggccagcg cccgctcgcg caacccgggc
    15121 aggggaagca cgcggggggc cgaagccgcg agcagcgtgg cgccatagcc gaactcccgg
    15181 agcgccgtct cgcactccgg gcacccctcg gcgaggtgcg cctccagccg cttccggtcc
    15241 ttctcctcga tcgtgccgag cgcgtacgcc gcgcacagct cgatcatctc ctccttgtgc
    15301 tccttgtgtg agctcacgtc cattcctcgc tgggcaacag cgcgcgcagc cgcgccagcg
    15361 cctgccgcgt ccaggatcgg accatgacgg cgggagcgtt catgcggccc gcgatctcca
    15421 gcgtggaaag gccgtcgaag aaggacgatt ccaggacgcg ccggtgcttc ggctccaggg
    15481 cgccgagggc gcgccgcacc cgctcggcca gctggcggcg ccccggcgag tcgtcgggat
    15541 cgtcggcgag agccgcgccc gcgtcgaaat cgccggcgcc tcggcggccg gaaaccgcgc
    15601 ctgccggaac gcggctcagc gcgcacgagc gcgcgatccc cagcagccac gcggccaccg
    15661 tgccccggtt caggtcgtag cgggcggact cgcgccaggc ccgcgtccac acttcgacca
    15721 gcgcctcgtc cgcctcgttc cggtcccccg tgatccggac catcaagggg tagaggagcg
    15781 gcgcatagcg gtcgtacagc gacccgaccg cgtcgcgatc cccttcttga agcctccgga
    15841 tcagctcgaa atcgctctcg ctcatggcgt cgcaccttac ccgatgcccc cctcccggac
    15901 aacgttccgc gcgcgtgcta ggcttttctc atggcaggca agcgtctcct catcctcggc
    15961 ggcggcttcg cggggctgga cgtggcccgt gcgatcggac gctcgcgggc cgcgcgcgag
    16021 tactgggaca cgctcctcat cgacaaggag aacttcttcc agttcaaccc gctcctgccc
    16081 gccgtggcgg tcggctccgt agagacgcgc cacatcgtct acccgctccg ggagatggcg
    16141 cgccaccggc acatccgctt ccacaagaac aaggccgtcc ggatcgatct ggatacgagg
    16201 caggtggtcc tgcacaacag cctggccgag ccctacgaca cgctggtcat cgcgatgggc
    16261 agcgtgacca actactacgg cgtcccgggc gcggagcaat acacccgccc cttcaagacg
    16321 atcgccgacg ccatgaccct ccgcgcgcgc gtcgtggagc tgttcgagat ggccgagcag
    16381 gccgagacgc tggagaagcg ccggcgcctg ctctccttcg cgctggtcgg cggcggcgtc
    16441 acgggggtgg aggtcgcggc cgagctgatc gagatggcgc gcgagacgct gctcccgcgc
    16501 taccccagca tccgcgccgc cgacatctcg gtcaccatcg tcgaaggggg agaccgcgtc
    16561 gtccccacgg cgcgccccga gcactcgcgg tacgtccacc gcttcctcga gcggcgcggc
    16621 gtgaaggtgc tgctccacac accggtgcgg cgcgtcgagc cgtaccgcgt cctcgccggc
    16681 gaggaccgcc gggtcgacgc cttcaccatc atctggacgg ccggcgtgca cccgcccgac
    16741 ctggtgcgcg acctccccgt ccggcaccat cgggacggcc gggtgctggt ggacgaccgc
    16801 ctccacccca cgcgcgagga cggcacgccg ctgcccgacg tctacgtgct cggcgattgc
    16861 gcggcttcgc cccgcccgga cggcacgatg cagcccgcgc tctcgcagac gggcatcgcg
    16921 atgggagcct tcacggggag gcagctggtg cgggtggctg aggggaagcc gttcgagcgc
    16981 ttcgcgttca aggatgccgg ctacatcatc tccctgggga agcactcctc cgtgctggag
    17041 ctgttcggga tcccgctctc cggcaagatc gcctggctcc tctgggctgg ggcctacctg
    17101 gtcaagatgg tcgggttccg gaagcaggtg gaggtcggaa tcgaccatct ctcccacctc
    17161 ttcttcgagc acgacacgtc gcagatcctg gcgcgccggg ccgtcctttc cgacgacgag
    17221 ctgaacctgt cgctggccga acccggccgg gacggctcgg aggcgcccgg tccgggagcc
    17281 gcatcggagg ggggctccca ggcatccaat ccacgcggag agctggtcac aacccccggc
    17341 gaaacccgta ggcgtacata ggggtaccac ctactatgtc cggatcgcat cacggtttcg
    17401 gacccatcat catggatgag gaggaggaat ccgaatgaag cgaacgactc tgattctcac
    17461 ggtggcgatc gcggcggcat gtctggcgtt cgcactcggc accgccctgg ccgccgacgt
    17521 gcacgccggg ggcaagccga ccacgctgag cggcgagctc gtggacatgg gctgctacat
    17581 cggccacatg gccaagggcg agaagcacgc cgactgcgcg gcgaagtgcg tttcgggcgg
    17641 gatgccgatg ggcgtcctga cctccagcgg aaagctctac ctgctcacga tggatcacgc
    17701 gaatccggac gcctacaaca aggccaagga cttcgcgggc aagcaggtca agatcacggg
    17761 cccgacccac gtccggaacg gcgtcaacac gatcgacgtc gtctccgccg aaacggtggc
    17821 gatggccaag tagagcagcg ccctgtcgcg cgggcggatc ccggtccgcc cgcgcacgga
    17881 ttcccccaga ttaacgcgta caccccacct tccgagctcg tccaggtagc ctgaggtggt
    17941 attccagccg gtccccccgg accggatgcg cgtcgccgct tcatccgcta ctcttccctt
    18001 catgaaacca ctcccccgga aacacctcat cgcggtagcg gcactgttga ccctggtggc
    18061 ggcggccccg ccgcccgccc cgaagggacc gaagaagcag gtcaccgtga cgggagagat
    18121 cctggacatg ggatgcttca tctcgcgcgg actgcgcgac gagctccacc gcgaatgcgc
    18181 gaccaagtgc ctcgcgatgg gcgtcccgat ggggctgttg ggagccgaca gcacggtcta
    18241 catcctgacg cagaatcacg accgcgccat ggaaccgtcg gcgttccagc ctccggaccc
    18301 gtttgcccag tgcaagggat gggcggcgaa acaggtcgcg gtcaccggat ttgtgtggga
    18361 gcgaaagggg tacaaggaaa tcgaagtgaa gacggctagg ctggcaccgg cgccgtctcc
    18421 gaaggtcccg tgagcctctc gactcgcggc cggattcctc tctccacgct ccccgcttcg
    18481 tggcggcgcc tcctcgtcgg gggcgccgcg ctgcttctgg tgaccgtggc gccgatcgcg
    18541 cgcgcccagg aaaccagcca gcaatgcctc gaccactcca aggaggccgc ggccgaagcc
    18601 aagaaggcga tggccgcttc caagggcaag acgacgctcg ccaaggcccc ggccgtcgcg
    18661 gcggcgcccg ctccccgggg tgccgcgccg gccaacgcag gcgcgcccgc ggccgcgaaa
    18721 gcgaaccccg cgggcaagcc gatgaagcgc acgctcacca ccctgcgcgg cgagctggtc
    18781 gactactact gctatatcga gaagggcgca cgcgggccgg agcacaagga atgcgccctg
    18841 cgctgcgtcg ccggcgacat ctgcatgggt ctgctgaccc ccgacgacca gctgatgatg
    18901 ctctcggtgc agcatctccg cgtgatggag cccctggcct ggcgcggcat ccccgatccg
    18961 ttcacgcagt gccgcggcct cttgaacgag accgtggatc tcaccggcta cgtgatgcag
    19021 cggaagggtc agaagatcat cgaagtgacg gccgtgaagg tcgtgaaggc cccgcccaat
    19081 cccaagtccg cggcctacaa gttcacgatt ccgacgccgc cgaagccggg accgaacgtg
    19141 gtccagcacc acgaaaagcg cgttccgacg accctccgcg gcgagctggt cgactactac
    19201 tgctacatcg agaagggcgc ccgcggcccc gagcaccgcg attgcgcggt gcgctgcgtg
    19261 gccggcgacg tgtgcatggg acttctcacc aaggacgaga agacgctcat gatgctctcg
    19321 gtgaaccatc tccgagcgat ggacccgcgc gcctggcgcg gcgtccccga tcccttcacc
    19381 acctgccgca gcctgttggc ggagaccgtg gacctcacgg gatacatcat cgagcggcac
    19441 gggcagaaga tcatcgaagt gacggacgtc aaggtagtgc cgaagagcta aagtcccggc
    19501 catcaggccg ggacaacttg taccgccggt caggatcggc ggccgataac caggatctct
    19561 tcgaggtaga accgcaaagc accgtcctca ccttccggac ggaggaactc ccgcatccca
    19621 ccccgcgaat cggtcagcat cgcccgcacg cgctccttcg tcgcggcatc caccggcatc
    19681 cggccgatcc acgcctcgaa atcccgggcc tttcggaacg cttcgtcgtg ctcgatcgct
    19741 agcccggccc gctgaaataa ctgccgccac tccgacgcgg ggagcgcacg gtcgtggctg
    19801 gggtcgcgtg ccttctccca cgcgttgatc gcggccgcgg cttcggggtc ctcggggacg
    19861 acgttgtcga tcaccgcgac caggccgccc gggcggacga cgcgcgcggc ttcacgtacg
    19921 aacgcggcga tgtcgggaaa gtggtgggcg gcgatgcggc aggtggcgag gtcgaaggag
    19981 gcgtcctcga acggaagcgc gccggcgtcg gcttcctgga agcggacgtt cgcgacaccc
    20041 ttctcgcgga tgaagcgctc agcggcggcc agcatcgggg cggtgaggtc ggtcgctacc
    20101 acctcgcgca ccagagggga cagtgcgagc gcggtgtgac cgccgcccgt cgcgacgtcc
    20161 agcgcgcgcc attcggaacg cggcgccgtg acctcgatca ggcgatcgag gctctcaccc
    20221 tgcgcgtggt ccttcgaccg gacgtagttc tcggcggaag cgccgaagcg gcgcttcacc
    20281 tcgtcgcggg tgtcgctcac ttcttcttgg cgcgcgctct ccgcggggtg cggacggcct
    20341 tcttcgtggc tcgaacggtg cgccccgcgc ggcgggcctt gcgccggccg cctccgcgcg
    20401 ctttcttgcc gcgcggcttc cggcccgtcg ccggagcctc gctcttcgcc gcggcgcgcc
    20461 gacctcgacg cccgcgcgtt tcgccgcccg cggccgcctt cagctcgcgg attcccttcc
    20521 cccggatcgc gcgccacgac gcgagcgccg agcggcgcgg gcgcgccttg cccgactccc
    20581 agagaaggat cgtctgaggg gagactccga ccagcttggc gtagacgcgg cggctcatgc
    20641 ccaggcgcga gcggtgcacc ttgacccagc gcggcgagaa gcggatctgc gtcccgtcct
    20701 cctcggatgc ggctgcggcc ggcgcggcgg cggcgcggcc cgacgcggcg cgcattgcgg
    20761 cgcgctccac ggcggccagc tgccgtttgg cctgtgccat gcggcggcgc agcgcggcca
    20821 cctggcgttt cagatcgcga accgcttcct tggtttcttt acgcgcgagg cgccggatct
    20881 cggcgcgcag cacgcccatc acgttcggca cgttgcctcc tgtcggtagc tggggtctat
    20941 cgacgccctg cggccgccgc ctcgctgggc ggctgtcccg ttctggattg gggtctgggt
    21001 tcgtggaagc gctcgcccgc cagcgccatc cgctgcaggc gcccgcaggg cccgtacggc
    21061 gcgccggcct gctggcgccg cgcgagccgc tccacgatgg tgccgatgcc cacggcgtcg
    21121 gcgtgacgca ggagcccgcc gcggaagggc gggaatcccg tgccgagaat catcgaaagg
    21181 tccacgtcgg cgggatcttc gaccacgcgg tcgtccagcg cgagcgccgc ctcgttcacc
    21241 atcgcgtcca ccatgcgatc ggcgatctcg gccgcgggca ccggctcgcg cgaggtggcg
    21301 agcgacgcga gcgccgggtt cacgccggag cgtcggccgc gctcgtagcg gtagaagccc
    21361 aggttcgcct tcttgcccag cgcgccctcg gagaccatct tgtcgatcag cgcgagcgaa
    21421 gggatccggt cgccgaaggc gtccagcagc acgtgcgcca ccttccgcgc gatatccatg
    21481 ccgatctcgt ccagcagctc cagcggcccc atcggcatgc cgaaatcgcg cgccgccttg
    21541 tcgatcgcgg gaatgctctg gccgcgctcc acgaagccga ccgcctcggc aaggtagggc
    21601 atcaggatcc ggttcacgag gaagcccggc ttgtcggcca cgatcacggg cgtcttcttg
    21661 agccgccgcg tcagcgccac cacggtgtcg agcgacgccg cgtccgtatc gtccgcgcgg
    21721 atcacttcga ccagcggcat gcgcgtggcc ggattgaaga agtgcatccc cacgacgcgc
    21781 tcggggtgct tcagcccgcg cgccatcgcg ctcacggaga gcgatgaggt gttcgtcgcg
    21841 aagacgcacg ccggcccgac ctgcgtctcc agctcgcgga agagcgcctg cttgatgttc
    21901 aggtcctcga ccaccgcctc cagcaccagg tccacgcggc ggaatccctg gagatccagc
    21961 gtcggctcga tccggcccat cgccatctcc atccccgacc gggtcaggcg atagcgcgag
    22021 acctcgcgct tgaagagcga ccgcgcgtgc gcgagcgact tcaagatcgg ctccggcttg
    22081 atgtcgcgca gccgcacgcg catgtcgcgc atgctcatga ggtaggcgat ctccccgccc
    22141 atggtgcccg cgcccagaac ggccgcggtc tcgacgcgcc gcgccttgtc ggcgggtggc
    22201 acgcgcttcg cgcgctcgtt catctggaag atgcggcgca gactctccga ttccggcgtg
    22261 aagaggagct tggcgaccgc cttgcgctcg atcttcagcg cctccagaat cggcttcccc
    22321 cacgaggcgc ccaccacgcg cacgatctcg agcggcgcgg ggtagtgccc cttcgtttcg
    22381 cggaggacgg tctggcgcgc ctgcccgagc gcgtaggaac ggaagggcgg aaccgactgc
    22441 atgatccggt tgggcagggc cggcgcggca cggcgcggcg ggctggggct ggagaggagc
    22501 gcctcggtcc acgcgaccgc gcgctcggag aagcgggccg cgggcagcac gtcgtcgagc
    22561 aggcccatct tccgcgcgcg gcgcgggtcc agcgagcgcc ccgtgagcaa gaggctcatc
    22621 gccggaatca agccgaccag gcgcggcagc cgcgtggtgc cgccgaaccc gggaaggatc
    22681 ccgagctgca cctcgggcag tccgatcgtc acgcccttgt cggccgcgat ccggtagcgc
    22741 atcgcgagcg ccagctcggt gccgccgccc aggcacgcgc cgttgatcgc ggccaccgtc
    22801 gggaagggaa gctccgacca ctcctggaac agcgtctgcg cttccttcac cttggcttcg
    22861 gcgtcggccg tgctcgcgat cgtgtcgaac tcgttcacgt cggcgccggc gatgaaattg
    22921 ccttccttgg cgctggtcag gaccagcgcg cgcgggaagg acgcgtcggc gcgcttcttc
    22981 agcgcgtcca gcgcgcggcg cagcgaggcg agcgcgttgc tgtcgaggag gttgaccttg
    23041 cgcgcgggat cgtcgaacca gagcaccgcg acgcccgagg cgcgcgcttc caggcggaag
    23101 gggccgaacg agccgggtgt gtcggcggcg ttcacgccgc gcgctccagc acgagggccg
    23161 cgccttggcc gccgcccacg cacagcgcgg ccagccccag cggcacgttg cggtgggcca
    23221 tctcgcggag cagcgtgagc acgagtcgcg tgcccgagac gccgaccgga tgtcccagcg
    23281 cgatcgcgcc gccgttcacg ttgaggatgg cgcggtcgat ctcgccgacc ggccgctcca
    23341 gccccagctc ttcgcggcac caccgcgccg aggccatggc cgtctcgcac ccgatcacct
    23401 gtgccgcgaa cgcctcgttg atctcgatca ggccgacgtc ggtgaagcgc gctcccgcgc
    23461 gttccagcgc gggcggaatc gcgtaggcgg ggcccagccc catgcgaacg ggatccagcc
    23521 ccacgaacgc gtagctcttg atgcgacctg ccggcgtgag gcccagctcg cgcgccttct
    23581 cggcggaagc gacgatcatc gcggcggcgc cgtcggtgat cgggcaggag ttccccggcg
    23641 tcacggtgcc gtagcggcga tcgaagaagg ggcgcagctt ggcgagcgcc tccagcgact
    23701 ggttcttgcg cggacccacg tcctcgatca ccgcctgctc gtagggcggc gcgaacaccg
    23761 gaacgatctc ctcggccaga cggcccgact cggtggccgc ggacgcccgg cggtggctca
    23821 tcagcgcgaa gtcgtcctgc gcctcgcggg aaatgccgaa gtccttggcg atgacctcgg
    23881 cggtctcgcc catgttgagc cccgagatcg gatcggtcag cccttccatg atggcgaccc
    23941 gcggcgcgaa gtccttcggc ttgatgcccg cgaacgcgcc gatccgctgg ccgagcgact
    24001 tggcgcgcga cgagcgctcg aacgcgcgcg tcatcccctc gctcacgatc agcgggatct
    24061 ggctcatgtt ctcggtgccg cccgccacga tcaggtcggc gttccccgcg gcgatgcgct
    24121 cgtgcgcctg caccagcgat tccatccccg aagcgcaatt gcgatgcacg gtcacggcgg
    24181 gaacgcgttc gggaattccg gcgagaagtg cgatgacgcg ggcgatgttg gcggaatcgg
    24241 agggctgcgc gacgttgccc aggatcactt cgtccacttc cgagggcggg acgccgtgac
    24301 gcgcgagaag ctcggcgatg gccacgcggc ccagctcgcg ggcgtgaacc ttggcgagcg
    24361 cggtgccgga cttggcgaag ggagttcgga atccgtcgat caggacgacg tcgcgcattt
    24421 cagaacaccc attgtgcggc cgatactcga taccggttgg ccatgcgagg gcagtgtgtc
    24481 acagtgcttc cgggaggtca atgcatggac gtgcaaacca ttccggaact gcttcgccac
    24541 gcgcgcgaaa cgcatcgtaa gccgaacgcg ttcttggtac gccgggcggg cgtctgggtt
    24601 ccggtttcca tcgagaccta tgcggcgcag gccgcctcgg ccggcgcctg gctgcgcgcc
    24661 cagggcgttc gtccgggcga tcgcgtggcg atcctgtcgg agagccgcta cgaatgggtc
    24721 gtcgccgaca tggcgatcgt ctccatggga gcggtctcgg ttcccttcta tccgacgctc
    24781 cccgccggcc agatcgcgcc gctggtggcc gactccggaa tcgtcggcgc gttcgtgtcg
    24841 agcaacgagc aggaggagaa gctcaagctg gtgcagtccg agcgcgccgg ctcctcggaa
    24901 gccctgcgct tcgtgtggaa tttcgatgcg tccggccttc cggaggcgcc tgccggcgct
    24961 ggcgatgtcc cgcttccccg ccccgacgac ctcgccacga tcatctacac gtcgggaacc
    25021 accggcacgc ccaagggcgt gatgctcacc cattcgaaca tcgtttccga agtcgtgatc
    25081 tccctgacca cctggaagct gaaccaggac gacgtctatc tctcctttct tcccttgagt
    25141 cacgtgctgg agcgcgccag cggtttcttc acgatgctgt acgcgggcgt gaccatcgcc
    25201 tacgcggaat cgttcgacaa gttgccgcgc gatttgcgcg aggtgcgccc caccgtgctg
    25261 atcgcggtgc cgcgcctgta cgaaaaggtg atcgcgcgcg ccgtcgagac cgcggctggc
    25321 gcgggccaat tcacgcgaag cattttccag aatgcgtacc gcgttgcggt ggaagtgggg
    25381 cgcctgaaga acgaggggaa ggccgttcca ccctggacga ttctcgagcg cgcgatcggc
    25441 ttcgccctgg tgtacggcaa gatcgcgcgc ggactgggcg gccgcacgcg gctccgcgtg
    25501 tcgggaggag cgccgttgaa tcgcgatgtc gccctgttct atcgcggcgc cggcctcgcg
    25561 gtgcacgagg gctacggcct gaccgagacc tcgtcggcga tcagcgtgaa cacgtccgag
    25621 cgccagcgcg tcggaacagt gggaccgctc ttccccggca tcgaggtgcg caccgccgaa
    25681 gacggcgagc tgttggtacg cggtccggtc gtcatgaagg gctactggca gaagccggag
    25741 gacacccgcg aggcgctgga gggcggctgg ctgcataccg gcgacatcgg agcgctggat
    25801 gccgacggtt ttctccgcat caccgaccga aagaaggacc ttctggtcac gtccgtgggt
    25861 aagaaggtgg cgccccagcc gatcgaggcg gcattgaagg cctcgccccg gatcacggag
    25921 gcgctggtcc tgggcgacgg ccagaagttc gtggccgcgc tgatcgtccc ggcagcgggc
    25981 gccacgcgcg aggcgatcgc cgaggacgtg gaccgcgtga acggcacgct ggcgcagttc
    26041 gagcggatca agcgcttcga gctgatcccc gacgacatga ccgtggagaa gggattgatg
    26101 acgccctcgc tcaagctcaa acgaaaggcc gtcgtggagc accatcgcga ggccgtggag
    26161 aagttgttcc ggggagcttg agctgatccg cgtccgtgtt ctcgccgttc tcctgtcgct
    26221 ctccgtggtg tcgctgggcg cggcgccgtc gcatcgaacc gcgcggccct cctcgcgcga
    26281 cgcgcgcccc ctgtccg
//
LOCUS       DASSLZ010000169         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_944293, whole genome shotgun sequence.
ACCESSION   DASSLZ010000169 DASSLZ010000000
VERSION     DASSLZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944293"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFM00_06605"
     CDS             <1..894
                     /locus_tag="VFM00_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497952.1"
                     /translation="TPEAGHLIHMPSHIYLRVGRYDDAADLNARAIRVDRDYLKRYDV
                     QTIYRMMYYPHNIHMRWSALCSAGRSAAAAAAAKELLDAVPWEMVQQMQPMEFFRAVE
                     YYTPARFGKWNEILALPAPPAGMLVTTGIWHYARGLALAATGRGTDAGIENDSLAAIA
                     ARLPADAAFGLNPARPLLDFASVLLQGEMAARAGSTEDAVRLLSQAAGMQDSLRYDEP
                     PPWYATARQALGAVLLGAGRTREAADVYREDLKRYPENGWSLYGLAQSVKASGEASGP
                     EMQRFKKAWEKADVEISESRY"
     gene            1391..2245
                     /locus_tag="VFM00_06610"
     CDS             1391..2245
                     /locus_tag="VFM00_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497953.1"
                     /translation="MTDLPARSFALPDLRVVPVDCLVPHERHDEHRMGPLTARLRSEA
                     VLRNPPIVTPVPASGEERYVVLDGANRSEAARAIGLPHFAVQVVPYEEPDVRLLTWNH
                     ALAGADAASLFQILSSVPGIACSRESQAHARALLARREVIAYAASTGGAIALSAPGDF
                     AEQNRALNRVVEAYREKTRVYRVGSDSVEAALERIPDAEAIVVFPRYAPAEVLELAAT
                     GERVPAGITRHLVRWRALRVNLPLDLLADRETSREEKQAWLRSWMRERVEQGHVRFYE
                     ESTVLFDE"
     gene            complement(2247..>3034)
                     /locus_tag="VFM00_06615"
     CDS             complement(2247..>3034)
                     /locus_tag="VFM00_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7497954.1"
                     /translation="VAGPIYPPAGGRPDIHWPPDGTAANAAEITKKAREYLGSGCDWV
                     KIYETGGTYDDTSGTPFYTSEEIRAAVEAAHPRGRWVAAHAMGLEGARRAVEAGVRSL
                     EHGSRLDAKTAREMARRGTYLVPTLYHLQWYSDHGAALGYAPGYAERLAALQKEQFAS
                     LALARRAGVRVGSGSDAVYSMHGENARELVWMVRAGLTPVEALRAATSTNAALLGLEH
                     EIGRIETGYAADLVAVPGDPSRDIQAVLAPSFVMKAGRVVLSP"
BASE COUNT          445 a         1090 c         1067 g          432 t
ORIGIN      
        1 acccccgagg ccggccatct gatccacatg ccgtcgcaca tctacctgcg cgtgggccgc
       61 tacgacgacg ccgcggacct caatgcccgc gcgatccgcg tcgaccgcga ctacctgaag
      121 cgctacgacg tccagaccat ctaccggatg atgtactacc cgcacaacat ccacatgcga
      181 tggtcggcgc tctgctccgc cgggaggagc gcggccgcgg cggcggccgc caaggagctg
      241 ctggacgcgg ttccctggga gatggtgcag cagatgcagc cgatggagtt cttccgcgcg
      301 gtggagtact acacgcccgc gcgcttcgga aagtggaacg agatcctggc cctgccggcg
      361 cctcccgcag gcatgctggt cacgaccggc atctggcact acgcgcgcgg cctggcgctg
      421 gccgcgaccg gccgtggcac cgatgccggc atcgagaacg acagcctggc cgcgatcgcg
      481 gcgcggcttc cggccgacgc cgcgttcggg ctgaaccccg cgcgcccgct tctcgacttc
      541 gcgtcggtgc tgcttcaggg ggagatggcg gcccgggccg gatcgaccga agacgccgtg
      601 cgcctgctga gccaggcggc cgggatgcag gactcgctcc gctacgacga gccgccgccc
      661 tggtacgcca ccgcgcgcca ggcgctcggc gcggtcctcc tcggagcggg aaggacgcgc
      721 gaagcggcgg acgtctatcg cgaggatctg aagcgctatc ccgagaacgg ctggtcgctg
      781 tatgggctgg ctcaatcggt caaggcttcc ggagaagcca gcggacccga gatgcagcgc
      841 ttcaagaaag cctgggagaa ggcggacgtc gagatcagcg agtcgcgcta ctaggccgac
      901 agatcgtcag gccggtcctg ccagggtgac agaaggaatg ggagcgacga atccggagcg
      961 gccatgggcc gctcgtaagt gccgcgcgcc caagggtccg gtccggcccg gacgggaccg
     1021 gcgccgggca tgccgattgc agtaggagat gagtggcggc gcgcagcggc agacggttcg
     1081 cgggagagcg cggcagggca gcagttggcc ggcgcggata catcctgcga tgcgcgtagt
     1141 ccaggagtca taccgaactt agcaagcgtc gatccgagcg gttgatgcct tgccggccgc
     1201 gtctggcggg ctgagactgc tccaccttgg cgtcgattag gatcgcgtat gatccggata
     1261 cggcaggctg ccccgcgcgc cggaattcat agagcgagag cccgcgacgt gcagtcgtcg
     1321 cgggcttttg cgttccggca tgctctggtc gcgcctcgcg cgaaccggga catggtagag
     1381 tcccgggccc atgaccgacc tcccggcccg aagcttcgcg cttcccgacc tgcgcgtggt
     1441 gcccgtggac tgcctggtcc cccacgaacg ccacgacgag catcgcatgg ggccgctcac
     1501 ggcgcggctt cggagcgagg cggtgctccg caatccgccg atcgtcaccc cggtcccggc
     1561 gagcggcgag gagcgctatg tggtcctcga cggcgccaac cggagcgagg ccgcgcgcgc
     1621 gatcggcctt ccgcacttcg cggtgcaggt ggtgccctac gaggaacccg acgtgcgcct
     1681 gctcacctgg aatcacgcgc tcgccggcgc ggacgcggcc tccctcttcc agatcctctc
     1741 gtcagtgccg gggatcgcct gttcccgtga atcgcaggcc cacgcgcgcg cgctgctcgc
     1801 gcgccgcgag gtcatcgcct acgccgcctc gaccggcgga gcgatcgcgc tctccgctcc
     1861 cggcgacttc gcggagcaga accgcgcgct caaccgggtg gtcgaggcct atcgcgagaa
     1921 gacccgcgtc taccgggtgg gctccgattc ggtcgaggcc gcgctggagc ggattcccga
     1981 cgccgaggcg atcgtagtct ttccgcgcta cgcgcccgcc gaggtgctcg agctggccgc
     2041 gaccggggag cgcgtgcccg cgggaatcac gcgccatctc gtccggtggc gcgccctgcg
     2101 cgtgaacctg ccgctcgatc tcctcgccga ccgagagacg tcgcgcgagg agaaacaggc
     2161 gtggcttcga tcgtggatgc gggagcgggt ggagcagggg cacgtccggt tctacgagga
     2221 gagcacggtc ctcttcgacg agtagctcag ggcgaaagga cgacccgccc ggccttcatc
     2281 acgaacgacg gcgcgagcac cgcctggatg tcgcgcgacg gatcgcccgg caccgccacg
     2341 aggtcggccg cgtagccggt ctcgatccgg ccgatctcgt gctccaggcc cagcagcgcg
     2401 gcgttcgtcg acgtcgccgc gcgaagcgct tcgaccggcg tcaggcccgc ccgcaccatc
     2461 cagaccagct cccgggcgtt ctcgccgtgc atcgagtaca cggcgtccga cccgctgccg
     2521 acccggaccc ccgcccgccg cgcgagcgcc agcgaggcga actgctcctt ctgcagcgcc
     2581 gccagccgct cggcgtaccc gggggcgtac ccgagtgccg cgccgtgatc cgaataccac
     2641 tgcaggtggt agagcgtggg cacgaggtag gtgccgcgcc gcgccatctc gcgtgccgtc
     2701 ttcgcgtcca gccgcgagcc gtgctccagg gagcgaacgc ccgcctccac cgcgcgccgc
     2761 gcgccctcca gccccatcgc gtgggccgcc acccagcgcc cgcgcggatg ggccgcctcc
     2821 accgcggcgc ggatctcctc gctcgtgtag aagggggttc cggacgtgtc gtcataggtg
     2881 ccgccggtct cgtagatctt gacccagtcg cagccgctcc ccaggtactc gcgcgccttc
     2941 ttcgtgatct ccgcggcgtt cgcggcagtg ccgtcggggg gccagtggat gtcggggcgg
     3001 ccgcccgccg gcggatagat gggccccgcc acga
//
LOCUS       DASSLZ010000170         9381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_174272, whole genome shotgun sequence.
ACCESSION   DASSLZ010000170 DASSLZ010000000
VERSION     DASSLZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9381
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174272"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2558
                     /gene="acnA"
                     /locus_tag="VFM00_06620"
     CDS             <1..2558
                     /gene="acnA"
                     /locus_tag="VFM00_06620"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019591241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HET7497955.1"
                     /translation="NPAAEPDREIAFAPARVLLQDFTGVPAVVDLAAMRDAMAALGGD
                     PSKINPLQPAELVIDHSVQVDEYGTKAALLLNAEKEFERNRERYLFLRWGQRAFRNFR
                     VVPPATGIVHQVNLEFLARVVMTTEGADPEAYPDTLIGTDSHTTMINGLGVLGWGVGG
                     IEAEAAMLGQPVSMLVPQVVGFRLSGSLKEGATATDLVLTVTEMLRKKGVVGKFVEFY
                     GPGLRTLPVADRATIANMAPEYGATCGIFPVDEETLRYLRFTGRAEAHVRLIEAYAKE
                     QGLFHREDSPEAEYTDRLALDLADVVPSMAGPRRPQDRVSLGGVKRNFSDALGSMKGS
                     PPKAPAPAQAGRWEAEGGSGTAATLAPPVHEVSLAGERFHLEDGAVVIAAITSCTNTS
                     NPAVMVGAGLLAKRAVERGLKTRPWVKTSLAPGSKAVTEYLNRAGLTPYLEALGFNTV
                     GYGCTTCIGNSGPLHEAISTAIEQGNLVVAAVLSGNRNFEGRIHPEVRANYLASPPLV
                     VAYALSGRMDRDLTTEPLGTDTSGTSVYLRDLWPTQREIDETIHGALHSETFRETYKD
                     VYTGDETWRNLPVPAGDRYAWEEASTYVKRPPYFDGMGRAPSPVTELRGARVLAYLGD
                     SVTTDHISPAGSIKRGSPAGIYLESHGVAPADYNSYGSRRGNHEVMVRGTFANVRIRN
                     RMVPGLEGGFTAHQPTGETMTIYDAAMRYAAEGIPTVVIAGKEYGSGSSRDWAAKGPK
                     LLGVRAVIAESYERIHRSNLIGMGILPLQFRPGDTAESLGLTGSETFDVDGVAGLAKA
                     SSRGGEAAVRARGEGGERTFRAIVRIDTPQEWRYFEHGGILPYVLRRLSGGPA"
     gene            2572..4524
                     /locus_tag="VFM00_06625"
     CDS             2572..4524
                     /locus_tag="VFM00_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7497956.1"
                     /translation="MSRGVSSFLRSGGLQLGLTALCAAAGIAGALVGRAHPHSTLHYV
                     LYGVAYLSGGFGPGRELIANLLARQLDINLLMVAAAAGSAALGHWGEGAVLLFLFSLS
                     GALERITMERTARSIESLVQLRPDTALVLRDGVEARVPVEAIAVGETVRTLPGERFAV
                     DGIVVEGSSSADESTLTGEAEPVEKEPGGYVYAGTVNLTGAPLVRVEKRPEETTLSRI
                     VRAVQEAGRAKSTAERIVDRWQRPYVIGVFAAAAIAGFVPWLLGASGLEAVYRGLVLL
                     VAASPCAVVIATPAAMLSAITHAAHHGVLFKGSVHLERLAGVTALAVDKTGTVTHGRP
                     RVVAVSEAAGAGDDKEILRLAASVEARSEHHHGRAVVAEAERRGLALAPVTEFESHAG
                     QGVHGHVEGFWVGVGREVLFQQHETPLPGEAASLLSGWREQGRTALLVLACEHPDGAA
                     NGNGRQPRWGAIAVADTIRPNARAAMAACRKLGIRHVALLTGDHEGVARAIGKEIGAD
                     EVRAGLHPEQKVSAVHELARRWGGLAMVGDGVNDAPALATATVGVAMGGAGTDVALDT
                     ADVVLMRDDLEGIPFAIWLARKARRVTAQSLSLAFGVIGILVASTLAGFLPLWLAVVC
                     HEGSTLLVIANGVRLLGIRHPEFDLA"
     gene            4521..5051
                     /locus_tag="VFM00_06630"
     CDS             4521..5051
                     /locus_tag="VFM00_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7497957.1"
                     /translation="MMPVPHGLVSLALPALLLAVVALSAPGCGRKAPQPPAATAPSSP
                     YDTGPRAGEFPVAERLAEKGRDLFKQKACTACHAFGRLLTGPDLRGVSMRRSARWMEM
                     QILHPDVMVTEDPITIDLYNTYLVRMANQGLTPDEARCVIEYLKWVDEDSTKAPVIPP
                     STNPPAAPPVTPTPGG"
     gene            complement(5048..5752)
                     /locus_tag="VFM00_06635"
     CDS             complement(5048..5752)
                     /locus_tag="VFM00_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7497958.1"
                     /translation="MALISESERVLQAIRATPLFRGLSSEDQKRLAALSALRNYDRGD
                     FLWRAGDPAEQLTIVVKGRVKIVTHGDTGDTILELFEEGEPVGAIAVYNYIPYPASAV
                     CMEPVVLLTLPRRDYFELLDRNPDFARAIIRELTKLVVAMARRLQELRGQKVDARIAQ
                     LFLTLAERLGKEVKGGIEIPIQLTRQEIADLVGTTVESSIRVLSRWGRERIVVSGENR
                     FLIPSREKLREIAEGS"
     gene            5954..6490
                     /locus_tag="VFM00_06640"
     CDS             5954..6490
                     /locus_tag="VFM00_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET7497959.1"
                     /translation="MEATTLIERLKRDHERVLRDVTELEGALARPAARVDGPAADDRI
                     ERVLAMLERQFQTHMAAEDQLLYPAIARAIPASAPSLEPLHDEHAELRQMLHRLQATL
                     RESASADRDEQIPIQVHDLADLLRLHIRKEESLVFHVAARLLAPEEIAAVLHRMNAEP
                     RGETAGEKRSLMKKGSSR"
     gene            6487..6963
                     /locus_tag="VFM00_06645"
     CDS             6487..6963
                     /locus_tag="VFM00_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7497960.1"
                     /translation="MNRSIAFASFLVLLLAAAGCGSKSEPQGAGPGSGATAVSPYDGG
                     PRAGDSPVEPAMATKGEALFKTKGCTACHAYDKRITGPALKGVTQRRTADWMEHQILH
                     PEIMTKEDPISKQLLATYMVQMSNQGLKPEEAKAVIEYLKKLDEESSETQQAGVGK"
     gene            6987..8888
                     /gene="nosZ"
                     /locus_tag="VFM00_06650"
     CDS             6987..8888
                     /gene="nosZ"
                     /locus_tag="VFM00_06650"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HET7497961.1"
                     /translation="MKRWMLAATVLVVLVGGAAMMQGCTKSLRGGGTAAARTYVKPGE
                     YDTYYAFLSGGHSGGVFVYGMPSCRYITTIPVFTPESAKGWGYDEESKAMLKGMNWGD
                     AHHPGLSETDGDYDGRWLFINDMPHARIARIDLSDFTTKEIYGPIPNISAAHACPFPT
                     PNTEYVFAASRFSIPLPYPTVAETKDYATKFKGIIAGVKVSPEGHMSLGFEILMPPFD
                     WDLADAGKGPSNGWEFFTCYNSEEAHDSLEIKASQNEMDYLAAVDWRAAQKAADSGEG
                     TMIGGVKVLDPKVTKGIVYLVPVPKSPHGVDVDPSGQWICASGKLQAEVTVHSFAKLM
                     AGIQAKKFEKEIDGIPVLAYNDVLEAKVPVGLGPLHTQFDGRGYAYTSLFLDSQIAKW
                     KIGPPWNVTDKIDVYYSIGHLMASEGDTRHPTGDYVVALDKLSKDRYLPVGPSHPEAA
                     QLIDIRGPKMELLYDFPTYPEPHYAQMIRAEKLKPKKIYTLSENTNPYAIKSADQARV
                     ERHGKRVDAYLLAIRTHYTPDLVQVAQGDTVYFHVTNLEQDADITHGFGILFSNCDMQ
                     VEPGETKTMKWVADKAGVTPFYCSNFCSALHQEMQGYIEVMPAGSRIASVSRPNREQL
                     AQVAALLRR"
     gene            8948..>9381
                     /locus_tag="VFM00_06655"
     CDS             8948..>9381
                     /locus_tag="VFM00_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497962.1"
                     /translation="MVRYLTGSFRSTERRLIVLAALVLLPVFFLPVLPIWEMHLRAPQ
                     YPEGLTLTIYSNTIQGDVDKINTLNHYVGMKAITPDDFAEFRYLPQLLTGFGLLALLA
                     ALLNRRWLAALGWVLFSAFAVYMFRDYVTWLYRYGHDLNPHAA"
BASE COUNT         1504 a         3296 c         3190 g         1391 t
ORIGIN      
        1 ggaatccggc ggcggagccc gatcgcgaga tcgcgttcgc gcccgcgcgg gtgctgctgc
       61 aggacttcac gggagtgccc gcggtcgtgg acctcgcggc gatgcgcgac gcgatggccg
      121 cgctgggagg cgatccgtcc aagatcaatc cgctccagcc cgccgagctc gtgatcgacc
      181 actcggtcca ggtggacgag tacgggacga aggccgcgct tctcctgaac gccgagaagg
      241 agttcgagcg aaaccgcgag cgctatctct tcctccgctg gggccagcgc gcgttccgga
      301 acttccgcgt ggtgcccccg gcgaccggga tcgtgcacca ggtgaatctc gagttcctgg
      361 cgcgcgtcgt catgacgacc gagggcgccg accccgaagc ctatcccgac acgctgatcg
      421 ggaccgattc ccacacgacg atgatcaacg gcctgggcgt gctgggctgg ggcgtgggcg
      481 ggatcgaggc cgaggccgcg atgctcggcc agccggtctc gatgctcgtg ccccaggtgg
      541 tcgggttccg tctttccggg tccctgaagg agggcgcgac cgctaccgac ctcgtgctca
      601 ccgtcaccga gatgctccgg aagaagggcg tcgtcgggaa gttcgtggag ttctacggtc
      661 cggggctccg cacgcttccg gtcgcggacc gggcgactat cgccaacatg gcccccgagt
      721 acggcgcgac ctgcggcatc ttccccgtcg acgaggaaac gctccgctac ctgcgcttca
      781 cggggcgcgc cgaggcgcac gtacggctga tcgaggccta cgccaaggag caggggctct
      841 tccaccgcga ggactccccc gaggcggagt acactgaccg cctcgcgctc gatctcgccg
      901 acgtggtgcc gagcatggca ggaccccgga gaccccaaga tcgcgtgtcc ctgggcggcg
      961 tgaagcgcaa cttctccgac gcgctcggct cgatgaaggg atccccgccc aaggcgccgg
     1021 ctccggcgca ggcgggacgt tgggaagcgg agggcggaag cggcacggca gcgaccctcg
     1081 cccctccggt ccacgaagtg tcgctggccg gcgagcgctt ccacctcgag gacggcgccg
     1141 tggtcatcgc ggcgatcacc tcctgcacga acacctcgaa tcccgcggtg atggtcgggg
     1201 ccggactcct ggccaagcgg gcggtggaac gcggcctgaa gacgcgcccc tgggtcaaga
     1261 ccagcctggc gcccggatcg aaggcggtca ccgagtacct gaaccgggcc ggcctcaccc
     1321 cgtatctcga agcgctcggc ttcaacaccg tgggctacgg ctgcaccacc tgcatcggta
     1381 acagcggacc tctccacgag gcgatctcga cggcgatcga gcagggaaac ctggtcgtgg
     1441 ccgcggtgct cagcgggaat cgcaacttcg aggggcggat ccaccccgag gtgcgcgcca
     1501 attatctcgc ctccccgccc ctcgtcgtgg cctacgcgct ttccgggcgg atggaccgcg
     1561 atctgaccac ggagcctctg ggcaccgata cgagcggaac gtccgtgtac ctgcgcgatc
     1621 tctggccgac gcagcgggag atcgacgaga cgatccacgg ggcgctccac tcggagacgt
     1681 tccgcgagac ctacaaggac gtgtacacgg gcgacgagac ctggcggaac ctccccgttc
     1741 ccgccgggga ccgctacgcc tgggaggagg cctcgaccta cgtcaagcgc ccgccctact
     1801 tcgacggcat ggggcgcgcg ccgtcgccgg tcacggagct ccgcggcgcg cgcgtcctcg
     1861 cctatctcgg cgacagcgtc acgaccgatc acatctcgcc ggcgggatcg atcaagcgcg
     1921 gaagcccggc gggaatctat ctggagtccc acggcgtcgc tcccgccgac tacaactcct
     1981 acggctcgcg ccgcggcaac cacgaggtga tggtgcgcgg caccttcgcc aacgtccgga
     2041 tccggaatcg catggtgccc ggcctcgagg gcggcttcac cgcgcaccag ccgacgggcg
     2101 agacgatgac gatctacgac gccgcgatgc gctacgccgc ggaggggatc cccacggtcg
     2161 tgatcgccgg caaggagtac ggctccggct cgtcgcgcga ctgggcggcc aagggaccca
     2221 agctcctcgg cgtgcgcgcg gtgatcgccg agtcgtacga gcggatccac cggagcaacc
     2281 tgatcgggat gggcatcctg ccgctccagt tccgcccggg cgacaccgcc gagagcttgg
     2341 gcctcaccgg ctccgagacc ttcgacgtgg acggagtcgc cggcctggcg aaggcgtcgt
     2401 cgcgaggggg cgaggcggcc gtgcgcgccc ggggcgaggg cggcgagcgg acgttccgcg
     2461 cgatcgtgcg catcgacacg ccccaggagt ggcgctactt cgagcacggc ggaatcctcc
     2521 cctacgtgct ccggaggctt tcgggaggtc cggcgtgagg cggccgaccg cttgagccgc
     2581 ggcgtctcct cgttcctccg atccggcggg ctccagctgg gtctcacggc gctctgcgcc
     2641 gccgcgggca tcgcgggggc cctggtcggt cgcgcccacc cccactccac gctccactac
     2701 gtcctgtacg gcgtcgccta tctgtccggc ggcttcggac cggggcgcga gctgatcgcg
     2761 aacctcctcg cgcgccagct cgacatcaac ctgctcatgg tggccgccgc ggcgggctcc
     2821 gccgcgctcg gccactgggg cgagggcgcg gttctcctct tcctcttctc cctgagcggc
     2881 gcgctcgagc ggatcacgat ggagcggacg gcgcgctcga tcgagtcctt agtccagctc
     2941 cggcccgaca cggccctggt gctccgcgac ggcgtcgagg cgcgcgttcc cgtcgaggcg
     3001 atcgcggtcg gcgagaccgt gcgcacgctt cccggcgagc ggttcgccgt ggacggcatc
     3061 gtcgtggagg gctcctcctc ggctgacgag tcgacgctga ccggcgaggc ggagccggtc
     3121 gagaaggaac ccggcgggta cgtctacgcc ggaacggtga acctgaccgg agcgccgctc
     3181 gtccgcgtcg agaagcgccc cgaagagacg accctgtcgc gcatcgtccg cgccgtgcag
     3241 gaggcgggaa gggccaagtc caccgcggag cgcatcgtgg atcgctggca gcgtccctac
     3301 gtcatcggcg tcttcgcggc ggcggcgatc gccggcttcg tcccctggct cctcggcgcg
     3361 tccggcctgg aggcagtcta tcgcgggctc gttctcctcg tcgcggcctc gccctgcgcg
     3421 gtggtcatcg ccacgccggc cgccatgctc tcggcgatca cccacgcggc ccatcacggc
     3481 gtcctgttca agggaagcgt ccacctcgaa cgcctggccg gggtcacggc gttggccgtg
     3541 gacaagaccg gcacggtgac gcacggccgt ccccgcgtcg tggcggtctc ggaggcggcg
     3601 ggcgccggcg acgacaagga gatcctccgg ctcgccgcgt cggtcgaggc gcgcagcgag
     3661 caccaccatg gccgcgcggt ggtggccgag gccgagcggc gtggcctggc cctggccccc
     3721 gtcaccgaat tcgagagcca cgccgggcag ggggtgcacg gccacgtcga ggggttctgg
     3781 gtcggcgtgg gccgcgaggt gctcttccag cagcacgaga cgccgctccc gggcgaggcc
     3841 gcgtcgctcc tctcgggatg gcgcgagcag gggcgcaccg ccctcctcgt gctcgcgtgc
     3901 gagcatcccg acggagcggc caatggaaac ggccgtcagc cgcgctgggg cgccatcgcc
     3961 gtcgccgaca cgatccgccc gaacgcgcgc gcggcgatgg cggcctgccg gaagctgggc
     4021 atccgccacg tcgccctcct gaccggcgac cacgagggcg tggcgcgcgc gatcggcaag
     4081 gagatcggag ccgacgaggt gcgcgccggc cttcatcccg agcagaaagt gagcgcggtg
     4141 cacgagctgg cgcgacggtg gggcgggctc gcgatggtcg gcgacggcgt gaacgacgcg
     4201 ccggcgctcg ctaccgcgac cgtgggcgtg gcgatgggcg gcgcgggcac cgacgtggcg
     4261 ctggacacgg cggacgtcgt gctgatgcgc gacgacctgg aggggatccc tttcgcgatc
     4321 tggctggcgc ggaaggcgcg ccgcgtgacc gcgcagagcc tgtccctcgc gttcggcgtc
     4381 atcgggatcc tggtcgcgag caccctggcc gggttcctgc ccctgtggct cgcggtcgtc
     4441 tgccacgagg ggagcacgct gctcgtgatc gcgaacggcg tgcggctcct cggcatccgg
     4501 cacccggagt tcgacctggc atgatgccgg ttccccacgg actcgtgtcc ctcgcgctcc
     4561 cggccctgct cctcgccgtg gtcgccctgt ccgcgcccgg atgcggccgc aaggcgcccc
     4621 agccgccggc ggcgacggcg ccctcctccc cctacgacac cggaccgcgc gcgggcgaat
     4681 ttccggtcgc ggagcgtctg gcggaaaagg ggcgggatct cttcaagcag aaggcgtgca
     4741 ccgcctgcca tgccttcggc cggctcctca ccggccccga cctgcgcggc gtgtcgatgc
     4801 ggcggagtgc ccgatggatg gagatgcaga tcctccatcc ggacgtgatg gtgacggagg
     4861 acccgatcac gatcgatctc tacaacacct acctcgtgcg gatggcgaac cagggactca
     4921 cgccggacga ggcgcggtgc gtgatcgaat atctgaagtg ggtggacgag gactcgacga
     4981 aggcacccgt catcccgccc tcgacgaacc cgcccgcggc gccgcccgtg acgccgacgc
     5041 ctggcggcta gctgccctcc gcgatctccc gcagcttctc gcgcgagggg atcaggaagc
     5101 ggttctctcc cgagaccacg atccgctcgc gcccccagcg cgacagcacc cggatcgacg
     5161 attccacggt ggtccccacg agatccgcga tctcctgccg cgtgagctgg atcgggatct
     5221 cgattccccc cttcacctcc ttgcccagcc gctcggcgag ggtcaggaag agctgcgcga
     5281 tccgcgcgtc cactttctgg ccgcgcaatt cttgaagccg tcgggccatg gccacgacca
     5341 gcttcgtcag ctcgcggatg atcgcgcgcg cgaaatcggg attgcggtcc aggagctcga
     5401 aatagtcgcg gcgcgggagc gtgagcagca ccaccggctc catgcagacg gcgctcgcgg
     5461 gataggggat gtagttgtag accgcgatcg cgcccaccgg ctccccctcc tcgaacagct
     5521 cgagaatcgt gtcgccggtg tcgccgtgcg tcacgatctt cacccgcccc ttgacgacga
     5581 tggtgagctg ctcggcggga tcgcccgcgc gccagagaaa atcgccgcga tcgtagttcc
     5641 ggagggcgga caaagcggcc agccgcttct ggtcctcgga ggacagcccc cggaagagcg
     5701 gcgtggcccg gatcgcctgc aacacgcgct cggactcgga gatcagcgcc atgcggcgcc
     5761 tcctggtatg acgggcgtca tacccacgag cttataccac gcgcaagccc ttgggagcag
     5821 ccgcgtttcg cccgggcggc ggtgcggaca atcttcgtcc ggtatgaccc ccgtcatcgc
     5881 ggcacggtgg cctccgggcc gaagattcct atcgttggta gtgaacgatg tcgcctcgcc
     5941 acggaggaca gtgatggaag ccacgacctt gatcgagcgc ctgaaacgcg accacgagcg
     6001 cgtgctccgc gacgtcacgg agttggaggg cgcgctcgcc cgtcccgcgg cacgggtgga
     6061 tggccccgcc gccgacgacc ggatcgagcg cgtgctcgcg atgctcgagc gccagttcca
     6121 gacacacatg gctgccgagg accagcttct ctaccccgcg atcgcgcggg ccatcccggc
     6181 gtcggcgccc agcctggagc cgctgcacga cgagcacgcg gagctccggc agatgctcca
     6241 tcgcctccag gccaccctcc gcgagagcgc gagcgccgat cgcgacgagc agattccgat
     6301 ccaggtgcac gatctggccg acctgctccg gctgcacatc cgcaaggaag agtccctggt
     6361 gttccacgtg gccgcgcgcc tcctcgctcc cgaagagatc gccgcggtcc tgcaccgcat
     6421 gaatgccgag ccccgcggcg agaccgcggg ggagaagcgt tccctcatga agaaaggaag
     6481 ctcccgatga accgctcgat cgccttcgcc tcgttcctcg tcctccttct ggccgccgcg
     6541 ggctgcggca gcaagtccga gccgcaaggc gccgggccgg gctcgggcgc gaccgcggtg
     6601 tcgccgtacg acggcggtcc gcgcgccggc gattctcccg tcgagcccgc catggccacc
     6661 aagggcgaag cgctgttcaa gaccaagggc tgcacggcct gtcatgccta cgacaagcgc
     6721 atcaccggtc cggcgctcaa gggcgtcacg cagcgccgca cggccgactg gatggagcac
     6781 cagatcctcc accccgaaat catgacgaag gaagacccca tctcgaagca gctgctcgcg
     6841 acctacatgg tgcagatgtc gaaccagggc ttgaagcccg aggaggccaa ggcggtcatc
     6901 gagtatctga agaagctgga tgaggaatcg tccgagacgc agcaggctgg cgtcggcaag
     6961 tgaccatgaa cccaaggggg ttgtccatga aacgctggat gctggcggcg acggtgctgg
     7021 tggtcctcgt cggcggcgcc gcgatgatgc agggctgcac gaagtcgctt cgtggagggg
     7081 gaacggccgc cgcgaggacg tacgtgaagc cgggtgaata cgacacgtac tacgcgttcc
     7141 tgtcgggcgg ccactcgggg ggtgtcttcg tctacggcat gccctcctgc cgctacatca
     7201 ccacgattcc ggtcttcacc cccgaatccg cgaagggctg gggctacgac gaggaatcga
     7261 aggcgatgct gaaggggatg aactggggcg acgcccatca cccgggtctc tccgagaccg
     7321 acggcgacta cgacgggcgc tggctcttca tcaacgacat gccgcacgcg cgcatcgcgc
     7381 ggatcgacct gagcgatttc acgacgaagg agatctacgg gccgattccg aacatctcgg
     7441 ccgcgcacgc ctgccccttc ccgaccccga acaccgagta cgtgttcgcg gcgtcgcgct
     7501 tctcgatccc gctcccctat ccgaccgtgg ccgagaccaa ggactacgcg accaagttca
     7561 aggggatcat cgcgggcgtg aaggtctccc ccgaaggaca catgtcgctg ggcttcgaaa
     7621 tcctcatgcc tccgttcgac tgggatctgg ccgacgcggg caaggggccg agcaacggct
     7681 gggagttctt cacctgctac aacagcgagg aagcgcacga ctcgctggag atcaaggcct
     7741 cgcagaacga gatggactac ctcgccgcgg tcgactggag agcggcccag aaggcggccg
     7801 actccggcga aggcaccatg atcggcggcg tgaaggtgct tgatcccaag gtcaccaagg
     7861 ggatcgtcta cctggttccc gtgcccaaga gcccccacgg cgtggacgtg gacccgagcg
     7921 gccagtggat ctgcgcctcg ggcaagctcc aggccgaggt caccgtgcac tcgttcgcca
     7981 agctgatggc cgggatccag gccaagaagt tcgagaagga gatcgacggc atcccggtgc
     8041 tggcctacaa cgacgtgctc gaggcgaagg tgccggtggg gctgggcccc ctgcacacgc
     8101 agttcgacgg ccgcgggtac gcctacacgt ccctcttcct cgattcgcag atcgcgaagt
     8161 ggaagattgg cccgccctgg aacgtgaccg acaagatcga cgtctactac tcgatcggcc
     8221 acctgatggc gtccgagggc gacacgcgcc acccgaccgg cgactacgtc gtggcgctcg
     8281 acaagctctc caaggatcgc tacctgccgg tgggcccctc gcatcccgag gcggcgcagc
     8341 tgattgacat ccgcgggccg aagatggagc ttctctacga cttcccgacc tatcccgagc
     8401 cgcactacgc gcagatgatc cgggccgaga agctcaagcc gaagaagatc tacaccctct
     8461 ccgagaacac gaacccgtac gcgatcaaga gcgccgacca ggcgcgcgtg gagcgccacg
     8521 gtaagcgcgt ggacgcctat ctcctggcga tccggaccca ctacaccccg gacctggtgc
     8581 aggtggcgca gggggacacg gtctacttcc acgtcaccaa cctggagcag gacgccgaca
     8641 tcacccacgg ctttggaatc ctcttctcga actgcgacat gcaggtcgag ccgggcgaga
     8701 ccaagacgat gaagtgggtc gccgacaagg cgggggtcac cccgttctac tgctccaact
     8761 tctgcagcgc gctgcaccag gaaatgcagg gatacatcga ggtgatgccg gcaggttcgc
     8821 ggatcgcctc ggtttccagg ccgaaccgtg agcagctggc ccaggtggcc gcgctgcttc
     8881 gccgctgaga ctcgaatgcg gggggccggc ggctgccggt tcccccgccc cgaaagggga
     8941 tgaagccgtg gtccgctacc tgaccggcag tttccgaagc accgagcggc gtctgatcgt
     9001 cctcgccgcg ctcgtgctcc tgcccgtctt cttcctgccg gtgctgccga tctgggagat
     9061 gcatctccgg gcgccgcagt atcccgaagg gctcacgctg acgatctact ccaacaccat
     9121 ccagggggac gtcgacaaga tcaacacgct gaaccactat gtcgggatga aagcgatcac
     9181 gcccgacgac ttcgcggaat tccgatacct gccgcagctc ctcaccggat tcggcctgct
     9241 ggcgctgctc gcggcacttc tcaatcgcag gtggctggcc gcgctgggat gggtgctctt
     9301 ctccgccttc gcggtctaca tgttccggga ctacgtgacg tggctctatc gctacggaca
     9361 cgacctgaac ccgcacgcgg c
//
LOCUS       DASSLZ010000171         7909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1317824, whole genome shotgun sequence.
ACCESSION   DASSLZ010000171 DASSLZ010000000
VERSION     DASSLZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317824"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="VFM00_06660"
     CDS             <1..1483
                     /locus_tag="VFM00_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497963.1"
                     /translation="IGVSVVAALAGLATWKGQDLLGVLPHAPRFEAVAVLPLQNLSGD
                     AAQDYFAEGMTDELTTQLARLGSLRVISRSSSAAARAQGGGTRVMAQKLHVNAMVEGS
                     VARSGDRVRISAKLIDAERDRYLWAQSYEREMKDVLALQGEVALAIAQQIGGRLSPGE
                     RERFTSDKRVLPEAFEAYLRGRYFWNRRTKGSLEQALAQFRRAAALDTAYAPAYAGIA
                     DVYVILDAYAGAPPGETFPRAREAALRALRLDDGLAEAHTALARVRLHYDRDLPGAQS
                     EFRRAIECNPGYATAHHAYSIYLRDVGRHEDAIAEAMRARALDPIAPIINANLGDTYF
                     YARRYDKAIAQHRETLAMDPEFGPSHLYLGSALERSGRFREALGEIERARSLVGDSTY
                     GLGPLGFTAARAGETGRAQAALRELTALQASGRASSADIALIEAGLGHRDAAIRWLTR
                     AYERRDPLNNLGVDPRFDTLRGDSRFHALLAGLGLEANVVAHP"
     gene            complement(1454..3361)
                     /locus_tag="VFM00_06665"
     CDS             complement(1454..3361)
                     /locus_tag="VFM00_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497964.1"
                     /translation="MARVSRLRVSVLIALLAGTGCASTHDRRASYLQLIHPDQRPPIG
                     EASAAGDSLFGPRGASERGDGITESRRPHLEALARRFAPTLVLPPGDAAKRGGRTHQL
                     LPTDPRLFGRVLQVDRIAAAPYGLSNSLSIPLTTLGTDSLTRLVESAREYRIRSDEIE
                     VWYFDFPGENPRAWWDAYERLRTGPDSAAWSVPTVWAHPFVEGGGRVVVQYWYFYPFN
                     DYLGNHEGDWEHVNVVLDAGSDSIAEVHYFFHERSVRLPQGRYAPSVQNGTHPVVFVG
                     GRANAVVNGPMRLLNGDHNSGSHGNFPFAGEWEAVAGLGHTESVYRADAESARVIPYD
                     RFRVVLVPDPGQLEYRSHPEVLRDWIWLLLPARWGYPSAPSLAGSFRLTDVGNRAPFG
                     PAYNSGWNRTAPGIGYASYNLRRVPRVQSYLEDLAQPWYYLYAFRHPRFVHDPRGLLE
                     RHELERLGLAPRGGRAERGLGSPVLGVQTGFPRGSFANNYGSSVGVSIWRNLWLKARF
                     GYLEFMGGYQRFRKEPIGSGTLYLYPLTAGLIVHGPEARVRPYASAGVGGYGWESRRR
                     LPASDTQYVNSGWGLGGNLGVGLEYYLRPKLALDVSARVHEAVSPGHEAGIRERRLRF
                     FTLWVGHYVRF"
     gene            complement(3808..7524)
                     /locus_tag="VFM00_06670"
     CDS             complement(3808..7524)
                     /locus_tag="VFM00_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7497965.1"
                     /translation="MKRRAKTDSATGDLFAATEPDLFAPAEEVVAPEPVALEPAAPEP
                     GVEIMTPKPQPPAPAMVPEPQAPAKPASRPRPRPTGPALADQDQRELIRGALDDTLVV
                     EAAAGTGKTTELVRRMIAILEGGRAELDRMVAVTFTDPAAGELKLRLRTAIEVARLDE
                     SRPVAARERLAAALPKLEEARIGTIHSFCADLLRERPVEAKVDPRFEVAPDDAARPLF
                     ERAFQRWFEGQLAHPSPGVSRILCRWKREPAFLYWGKDEGPRGLLRRAAWSLIGRRDF
                     PTPWRHAEGFERDRAIDAILEEMEALAAWGPRGNENDWFVKSLVEIGKYVDEVRRMER
                     VRDRDYDALEAKLTEWSKVSKNKHWTHKGWATSRDPEFDQQGLRSRRNALKAALDRFV
                     SDAGADLAPRLRDELWPVVEAYEKAKETAGCLDFEDLLLRARDLVRGNAAVRVELQRR
                     FTHYFVDEFQDTDPLQAEVLMLLAADDPELTAWRQARPVPGKLFIVGDPKQSIYRFRR
                     ADVALYREVQRQLVTHGALPVNLTVSFRSVPEIQTAVNAAFEARLGGASGGEYVPLEP
                     FRDAFAEQPPVIALPIPAPYSPWGKIVNWRIDESLPDVVAAWIDWLVKESGWTVTERE
                     KPDERVKVEPRHVCVLFRRFRSGPVDVTRPYVTALEARMLPHLLVGGSSFHEREEVEA
                     IRNALTAIERPDDELAVFATLRGPLFAVSDAALLTWKERVGGLHPFRTVPPAVAGAGE
                     APSALTEVADGLAILRDLHRTRNHRPVADTIANLLEATRAHAALAIWPTGMQALANVG
                     RLMDLARRAEQQGLVSFRAFVDRLEEDAERGEAAEAPLLEEGIQGVRLMTVHKAKGLE
                     FPIVILADMTAKETFEEPGRWSDPARGLFVQKLAGCSPPELIEHAGEEMEREREEAVR
                     LVYVAATRARDALVVPVVGDEPFDGWLKTLNPVVYPDVSRSKRPESLQAPGCPLFGGD
                     SVPGRPDAMQPPIISVTTGVHVPAVGDHRAVWWDPLTLRLGVRPTLGLAQSEILKADE
                     EGRSAEALANWEAWRWERDETRAEGRKPTRVVRAATEWVRATDTKVEGADMVVVMDSP
                     WRGPRPKGPRFGTLVHAVLSVVDLADSSGVGAHAAVQARILGATEEEAEAAARVVEAT
                     LAHPVLRRAAAAASRGECRRETPLVLRLEDGTLLECVADLAFPEEGRWTVVDFKTDTD
                     LGTMAESYKRQVALYAQGIAQATQKEARGIVLRV"
     gene            complement(7521..>7909)
                     /locus_tag="VFM00_06675"
     CDS             complement(7521..>7909)
                     /locus_tag="VFM00_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET7497966.1"
                     /translation="TDHKTGKARVDAGAVVWGGRTLQPLLYALAVEELLQGRVLSGRL
                     YYCTSDGGFEERDIRLDEDNRAAARAAIGVIGRALEQGFLPAAPRPDACRWCDYRPVC
                     GPNEEVRTARKTRERLEDLDYLREMQ"
BASE COUNT         1205 a         2752 c         2758 g         1194 t
ORIGIN      
        1 gatcggcgtc tctgtggtcg ctgccttggc ggggctcgcc acgtggaagg gccaggacct
       61 cctcggcgtg ctgccacacg cgccccgatt cgaggcggtc gcggttcttc cgctccagaa
      121 tctctccgga gacgcggccc aggactattt tgccgaggga atgacggacg agctgaccac
      181 gcagctcgcg cgcctgggca gcctgcgtgt catctcacga agctccagcg cggcggcgcg
      241 cgcacaggga ggcggaacgc gcgtcatggc gcagaagctg cacgtgaacg cgatggtcga
      301 gggatccgtc gctcgctccg gagatcgtgt ccgcatctcg gccaagctca tcgacgccga
      361 gcgggaccgc tatctctggg ctcaaagcta tgagcgcgag atgaaggacg tcctggcgct
      421 ccagggagaa gtcgcgctgg ccatcgcgca acagatcgga gggcgcctct caccggggga
      481 gcgggagcga ttcacatcgg acaagcgtgt cctccccgaa gccttcgagg cgtacctacg
      541 gggacggtac ttctggaatc gaaggaccaa gggttcgttg gagcaagctc tcgcgcaatt
      601 ccggagagcg gccgcgctcg atacggcata cgcccccgcg tacgccggga tcgccgacgt
      661 ctacgtgatc ctcgacgcgt atgcgggggc gcctcccggc gaaacgttcc cgcgagcccg
      721 cgaggcggcc cttcgcgcgc tgcgcctcga cgacggactg gccgaggccc ataccgcgct
      781 ggcgcgggtg cgtctccact acgatcgcga tcttccaggc gcccagtccg agtttcggcg
      841 cgcgatcgaa tgcaacccgg gctatgcgac ggcgcaccac gcctactcga tctatctgcg
      901 cgacgtgggg cgccacgagg atgcgatcgc cgaggccatg cgggcgcgcg cgctggatcc
      961 catcgctccc atcatcaacg cgaatctcgg cgacacgtac ttctatgcgc ggcgctacga
     1021 caaggccatc gcccagcatc gcgagaccct ggcgatggac cccgagttcg gtccgagtca
     1081 tctctacctc ggctccgcgc tcgagcggtc ggggcgcttt cgggaagcgc ttggggagat
     1141 tgagcgggcg cgttctctgg tgggggacag cacgtatggg ctcggcccgc tcggctttac
     1201 ggccgcgcgg gccggcgaga ccggacgggc gcaggccgcc ttgcgtgagc tcacggcgct
     1261 ccaggcctcc gggcgagcct cttccgccga catcgctctg atcgaggcgg gcctcggcca
     1321 tcgcgacgcc gcgatccggt ggctgacccg ggcctacgag cggcgggatc cgctcaacaa
     1381 tctcggcgtg gacccgcggt tcgacacgct ccggggcgat tcgcggttcc acgcgctgct
     1441 cgccggtctt ggactagaag cgaacgtagt ggcccaccca tagcgtgaag aaccgtagcc
     1501 gccgctcgcg gattcccgct tcgtggcccg ggctcacggc ctcatgcacc cgcgccgaca
     1561 catcgagagc cagcttcgga cgcaggtagt attcgagtcc gacgccgagg ttgccgccca
     1621 atccccatcc tgagttcacg tactgcgtgt cgcttgccgg gagccgccgg cgcgactccc
     1681 agccatagcc tcccacgccc gccgacgcat acggtcgcac gcgcgcctcg ggtccgtgga
     1741 cgatcaggcc ggccgtgagg ggatagaggt agagcgttcc ggacccgatc ggctcctttc
     1801 ggaagcgctg atagcctccc atgaactcca ggtagccgaa ccgggccttg agccagaggt
     1861 tcctccagat cgaaactccc accgaggacc catagttgtt cgcaaacgat cctcgaggga
     1921 agcccgtctg cacgccgaga acggggcttc ccaacccccg ctccgcgcgg ccgccgcggg
     1981 gcgccaggcc gagccgctcc agctcgtggc gttccagaag acctcgggga tcgtggacga
     2041 aacgcgggtg gcggaacgcg tagaggtagt accacggttg cgcgaggtct tcgaggtagg
     2101 actggacccg cggtacgcgg cggaggttat agctcgcgta cccgatgccg ggcgcggtgc
     2161 gattccagcc cgagttgtac gcagggccga agggagcccg attgccgacg tcggtcaacc
     2221 ggaacgaacc ggcgagggag ggagcgctgg ggtagcccca gcgcgctgga aggagcagcc
     2281 agatccagtc gcgaagcacc tcgggatggc tccggtactc gagctggccc gggtcgggaa
     2341 cgaggacgac gcgaaagcga tcgtagggga tgacgcgcgc cgattcggca tcggcgcgat
     2401 agacgctctc cgtgtggccg aggcccgcca ccgcctccca ctccccggcg aaggggaaat
     2461 tgccgtggga tcccgagttg tggtcgccat tcaagaggcg catcggaccg ttgacgaccg
     2521 cgttggcgcg tccgccgacg aagacgacgg ggtgcgtccc gttctggacg ctgggcgcgt
     2581 agcggccctg cggaaggcgg acgctgcgct catggaagaa gtagtgcact tccgcgatgg
     2641 aatcggagcc ggcatcgagg acgacgttga cgtgctccca gtccccctcg tggtttccca
     2701 ggtagtcgtt gaacgggtag aagtaccagt actgcacgac gacccgcccg ccaccctcga
     2761 cgaaggggtg cgcccacacg gtcggaacgc tccaggccgc gctgtcgggg ccggtgcgca
     2821 atcgctcgta ggcatcccac cacgcccggg ggttctctcc cgggaaatcg aagtaccaga
     2881 cctcgatttc gtcggagcgg atccgatact ccctggcgga ctcgacgagg cgcgtgagcg
     2941 agtccgtgcc gagggtggtg agaggaatcg acagggaatt gctcagcccg tagggagccg
     3001 ccgcgatacg gtcgacctgg agaacgcggc cgaagagccg gggatcggtg ggcaggagct
     3061 gatgggtccg gcctccccgc ttcgccgcgt caccgggcgg gagcacgagc gtgggcgcga
     3121 agcgccgggc gagcgcctcg aggtggggcc gacgggattc cgtgatcccg tctccgcgct
     3181 cactggcgcc ccgtggcccg aacagcgagt cgccggccgc ggacgcctcg ccgatcggtg
     3241 gacgctggtc gggatggatc agctggagat acgaggcgcg ccggtcgtgc gtggaagcgc
     3301 acccggtgcc ggcgaggagt gcgatgagga cggaaacccg gaggcgcgag acgcgagcca
     3361 tgacgttacc ccctgcttcg gaggaacggg cgcgccgcgg ctgcgtgtgc ggcgataggg
     3421 atggtgcggg agtatagcgc gaacgctaac ggggattcgc cacgttccgc tacccgttcg
     3481 cctcgggcgc acgacgtcgc ttgcttcggc ggaagcccgg ctggcgagtc agttccttac
     3541 gtactgtcgc gatcggtagt cggtgatccc caagcccgta ccgagtggtc ttcgcgatcc
     3601 ggcaaccact gccggtgaaa ggcctcagca tcttcttggc gtttgaaaac gcacttcatc
     3661 acccagccac ttgtaaccct atacccgaca gcgcgaatga ccatggtttc ggggaagctc
     3721 gcttcaatcc agtcgagcac cggctgaggg tccaaggtga ataggtccac acggtagcaa
     3781 gccatgagtc ctcatccggt ctagccgcta gacccgcaac acgatgcctc gcgcttcctt
     3841 ctgcgtcgcc tgggcgattc cctgcgcgta cagggcgacc tggcgcttgt agctctcggc
     3901 catcgtgccc aggtcggtat cggtcttgaa gtccaccacc gtccagcggc cctcttcggg
     3961 gaacgcgagg tcggcgacgc actcgagcag cgtgccgtcc tccaggcgca gcacgagcgg
     4021 ggtctcgcgg cggcactccc cgcgcgaagc ggccgccgcg gcgcggcgga gcacgggatg
     4081 ggcgagcgtc gcttccacga cgcgcgcggc ggcttcggcc tcctcctccg tagcgccgag
     4141 aatcctggcc tggacggcag cgtgcgcgcc gacgccggag gaatcggcga gatccacgac
     4201 cgagagcacg gcgtggacca gggttccaaa acgcgggccc ttgggcctcg ggccccgcca
     4261 cggcgaatcc atgaccacca ccatgtcggc gccctcgacc ttcgtgtcgg tggcccgcac
     4321 ccactccgtg gccgcgcgca cgacgcgcgt gggcttcctt ccctcggcgc gcgtctcgtc
     4381 gcgctcccac cgccatgctt cccaattcgc gagcgcttcc gccgaacggc cctcttcgtc
     4441 cgctttcaag atctccgatt gcgccaggcc cagcgtggga cggaccccga ggcgcagcgt
     4501 cagcggatcc caccagacgg cccgatggtc gcccacggcc ggcacatgca cgcccgttgt
     4561 caccgagatg atcgggggct gcatggcgtc ggggcgaccg gggacactgt ccccgccgaa
     4621 caacggacag cccggcgcct gcagcgactc gggacgcttg gagcgcgaca cgtcggggta
     4681 gacgacgggg ttcagcgtct tcagccagcc gtcgaagggc tcgtcgccga cgaccggaac
     4741 gaccagggcg tcgcgcgcgc gcgtcgcggc cacgtagacg agccgcaccg cttcctcgcg
     4801 ctcccgctcc atctcttcgc cggcgtgctc gatcagctcc ggaggagagc agcccgccag
     4861 cttctgcacg aagaggccgc gcgcgggatc gctccagcgg cccggctcct cgaaggtctc
     4921 cttcgcggtc atgtccgcga ggatcacgat cggaaactcc agccccttcg ccttgtggac
     4981 ggtcatgagg cgcacgccct ggatcccttc ctcgagcagc ggggcctcgg ccgcctcgcc
     5041 gcgctcggcg tcttcctcca gccggtccac gaaggcgcgg aaggagacca gcccctgctg
     5101 ttcggcgcgc cgtgccaggt ccatcagccg gccgacgttc gcgagcgcct gcatccccgt
     5161 cggccagatc gcgagcgctg cgtgggcacg cgtggcttcg agcaggttcg cgatcgtgtc
     5221 ggccacgggg cgatggttgc gggtgcggtg caggtcgcgc aggatcgcga ggccatcggc
     5281 cacctcggtc agcgcggacg gcgcctcgcc ggcgccggcg accgcgggag ggaccgtgcg
     5341 gaagggatgc agccctccca cgcgttcctt ccaggtgagc agcgccgcgt cgctcacggc
     5401 gaagagcggc ccgcggagcg tcgcgaacac cgcgagctcg tcgtcgggcc tctcgatcgc
     5461 ggtcagcgcg ttccggatcg cctcgacctc ctcgcgctcg tggaacgagc tgccgccgac
     5521 caggagatgc ggaagcatgc gcgcttcgag cgcggtcacg tacggccgcg tcacgtccac
     5581 cgggccggag cggaagcggc ggaacagcac gcagacgtgc cgcggctcga ccttcacccg
     5641 ctcgtcgggc ttctcgcgct cggtgacggt ccagccgctt tccttgacga gccagtcgat
     5701 ccaggccgcc accacgtcgg ggagcgactc gtcgatccgc cagttcacga tcttgcccca
     5761 ggggctgtag ggagccggga tgggcagcgc gatcacggga ggctgctctg cgaacgcgtc
     5821 gcggaacggc tccaggggca cgtactcgcc gcccgacgcg ccacccagcc gcgcctcgaa
     5881 cgcggcgttc accgccgtct ggatctcagg caccgagcgg aagctgaccg tgagattcac
     5941 gggcaatgcg ccgtgggtga ccagctgccg ctgcacctcg cgatagagcg cgacgtcggc
     6001 acggcggaag cggtagatcg actgcttggg atcgccgacg atgaagagct tcccggggac
     6061 ggggcgcgct tgtctccagg cggtcagctc gggatcgtcc gcggcgagga gcatgagcac
     6121 ttccgcctgg agcggatcgg tgtcctggaa ctcgtccacg aagtagtggg tgaagcggcg
     6181 ctgcagctcc acgcggacgg cggcgttccc ccggaccagg tcgcgggcgc gcaggagcag
     6241 gtcctcgaag tcgaggcatc ccgccgtctc cttcgccttc tcgtacgcct cgaccaccgg
     6301 ccacagctcg tcccggagcc ggggcgcgag gtcggctccc gcgtcggaga cgaaacggtc
     6361 gagggcggct ttcagggcgt tgcgccggga ccgcaatccc tgctgatcga attcgggatc
     6421 gcgcgacgtc gcccagccct tgtgggtcca gtgcttgttc ttggagacct tcgaccactc
     6481 ggtgagcttg gcctcgagcg cgtcgtagtc gcgatcgcgc acgcgctcca tccggcggac
     6541 ctcgtcgacg tacttgccga tctcgacgag cgacttcacg aaccaatcgt tctcgttgcc
     6601 gcgcggcccc cacgccgcca gcgcttccat ttcttccagg atcgcgtcga tggcccggtc
     6661 ccgctcgaac ccttcggcat ggcgccacgg ggtggggaag tcgcgccggc cgatcaggct
     6721 ccaggccgcg cggcgcagga gcccgcgcgg cccctcgtcc ttgccccagt aaaggaaggc
     6781 cggctcgcgt ttccagcggc agaggatccg gctcacgccg ggcgatggat gggccagctg
     6841 gccctcgaac cagcgctgga aggcgcgctc gaagaggggc cgggccgcgt cgtcgggagc
     6901 gacctcgaag cgcggatcca ctttggcttc gacgggccgc tcgcgcagga gatcggcgca
     6961 gaaggagtgg atcgtcccga tgcgcgcctc ctccagcttg gggagcgcgg ccgcgagacg
     7021 ctcgcgggcg gcgaccgggc gcgactcgtc gagccgcgcc acctcgatcg ccgtgcggag
     7081 ccggagcttc agctcgcccg cggccggatc ggtgaaggtc acggcgacca tgcggtccag
     7141 ctcggcgcgg ccgccttcca gaatcgcgat catgcgccgc accagctcgg tggtcttgcc
     7201 ggtgcccgcg gcggcctcga cgacgagcgt gtcgtcgagg gcgccgcgaa tgagttcgcg
     7261 ctggtcctga tcggcgagcg cgggtcccgt gggcctgggg cggggtcgcg aggcgggctt
     7321 tgccggagcc tgcggctcgg gcaccatcgc cggtgccgga ggctggggct tgggcgtcat
     7381 gatctcaacc cccggctccg gggccgccgg ctcaagcgcc accggctccg gggccacgac
     7441 ctcctccgcc ggcgcgaaga gatcgggctc ggtcgccgcg aacaggtcgc cggtcgccga
     7501 atcggtcttg gcgcgccgtt tcactgcatc tccctcaggt agtccaggtc ctcgagccgc
     7561 tcgcgcgtct tccgcgccgt gcggacctcc tcgttcgggc cgcagacagg acggtagtcg
     7621 caccagcggc acgcgtccgg gcgcggcgcg gcgggaagga atccctgctc cagcgcgcgt
     7681 ccgatcacgc cgatcgcggc ccttgccgcg gcacgattgt cctcgtcgag ccggatgtcc
     7741 cgctcctcga atccgccgtc ggacgtgcag tagtacaacc gccccgagag gacgcggccc
     7801 tggagcaact cctcgacggc gagcgcgtag aggagcggct gcagggtccg tccgccccag
     7861 acgacggcgc ccgcgtccac gcgcgccttt cccgtcttat ggtcggtga
//
LOCUS       DASSLZ010000172         8686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_174484, whole genome shotgun sequence.
ACCESSION   DASSLZ010000172 DASSLZ010000000
VERSION     DASSLZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8686
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174484"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFM00_06680"
     CDS             complement(<1..586)
                     /locus_tag="VFM00_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497967.1"
                     /translation="MAIPRIDRAARPRDNASRAPRVAARRRKFLRAFPGGFRDETYLE
                     WERNYKWEAHQRWMELLPATVWRGLILQGAFHEVASRAVTIESRTNLLFSFEKMALRD
                     AVKPVAGARAFAEGLYEFLHGRGAIERRFERWRDVVADLPRKQTRVLTWPIVTVFGFL
                     ADPDTHIFLKPNVTRVAAERYGFPFRYESRPSWPV"
     gene            complement(735..1019)
                     /locus_tag="VFM00_06685"
     CDS             complement(735..1019)
                     /locus_tag="VFM00_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497968.1"
                     /translation="MLIVRESFVAKPGMASKLAKHMKEVMAAQPKWKTRIMTDAVGTF
                     NTVVMETEVEDLAALTARMKEYEERTDLREKMKGYTEMWVSGKREIFQTV"
     gene            complement(1239..3089)
                     /locus_tag="VFM00_06690"
     CDS             complement(1239..3089)
                     /locus_tag="VFM00_06690"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HET7497969.1"
                     /translation="MKPLLVYDGDCGFCRLWIERWRATTGDRVEYATYQEVADRFPEV
                     PRERFAAAVQLREPDGKWSGGAEAVFRAQATAPGRRWPLWLYRRVPGVRPLTEAAYRL
                     VAANRGPLYRVTRWIWGDHVAPPGETLTAWIFLRLLAVVYLVAFVSMWVQVQGIAGPD
                     GILPARDFLAALHAKYGRLAFWIAPTLGWLGTSAGFLNAMCAAGVALSVGLVLGAAPI
                     ACLAGLWLTYLSLSILGQDFFWFQWDSLLLETGLLAILIAPWRGWSRPWNDAPPRRSG
                     LWLMRWLLFRLSFSSAAVKLLSGDPSWHARTALQYHFETQPLPPWTAWFAHHLSAGLL
                     RASSTGTLILEGIVPFLVFLPRRIRFVGVGLIASLQILILVTGNYGFFNWLTLALCVL
                     CLDDGVWPRGWRERTARWSANRRGSWFGWAIRPLAVVFFLLSLVPFLSTLHAPTAWLG
                     PVEYVSDLVWPVRSFNRYGLFAVMTTRRLEIVLEGSADGVRWLPYELRWKPGDPARRP
                     EFVAPHQPRLDWQMWFAALSNFQNQPWFLQLCRRLLEGSQPVAALFATDPFPRAPPKY
                     LRAVVYDYHFTTAEERKRTGAWWRREQLGLYCPVLALQNGQLVAVGSTSP"
     gene            3252..4205
                     /locus_tag="VFM00_06695"
     CDS             3252..4205
                     /locus_tag="VFM00_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7497970.1"
                     /translation="MLSTRGLVKVYPGPVTALNGVDLDVPPGMFGLLGPNGAGKSTFM
                     KILAGLLEPTSGTATLDGIDIVKDPDSVSPRLGYLPQEFGFYPHLTGEAMLGHLLDLK
                     GVSAPQGKKKLVAELLDRVNLSFAARRAVKGYSGGMRQRLGIAQAIAGNPRLIIVDEP
                     TAGLDPEERLRFYHLLSELAADRIVLLSTHIVEDVAVLCPRFAVIRTGRLIALTTPAE
                     ARAAIAGRMFEGLVSVEDLPRLRAERMVTQALLVEGKNRARIFEPAGTPPPGFVPAPA
                     TLEDAYLVLMRQGELPNGNGSSPAPASGRVAGTNAAIEAIG"
     gene            4202..7900
                     /locus_tag="VFM00_06700"
     CDS             4202..7900
                     /locus_tag="VFM00_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET7497971.1"
                     /translation="MSPRRLFAVFLVEFGHSFKRPLFYIMAFLIALISFGLSSGQMQI
                     SSGDSSVGGTKAWITSEFAQTQTMTYLTILIFAGFIAAAAGMTLLHDRETKVDVILHS
                     TPLKAGEYVWGRLLAVVGMFAVLLAWLALTAAFFNHLVPNGNALEIRGPFAWASYFRP
                     VLFVGLPFVVFFAGLSMWIGERTRSAVIVYLLPALAILVSGFFLMTWSPSWLSYGMNR
                     VLQVVEPSGYRWLNETLLKVDRGVKYYNTQPVPYDAIFWLNRLWMLFVGFGGILLTQR
                     SIARSRRGVESARARTKSAAARPEALGAGWDETAVRPSIQGIGMTSRVPGALATVAAV
                     ARTETKELLRQPALYLFMLIILVQVLGNSLLAVGAFDTPVLLTPGTSAVALANQSVTF
                     ICLLLMYYTVVSLEREHTTGLAPVLYAAPVRTGALLFGKALANGVVGAIVLVASLVGS
                     WLAISIQHTVPFSLGPYLLVWGLLLVPTFMFWTALVTAVYALVGNRYTTIAIALGILA
                     WTGWRALTKQISWAGNWALWGALRWSDLGPFQHDRLVLVLNRAMVLSAAFLFIALAVR
                     LFRRRSPDAVRTIHRLSPRSLRRSAFRLAPYALIPAALWITLLFQVSQGTEGGAAKKA
                     RKDYWAKNLKTWMDAPLPDIARADIAVKVDPQGNRLESRGALTFVNGLDTTLTQIPLT
                     GGTEWKNVTWTMNGRAVTPEDSKRLYVFTPPRPLAKGDSVVIGWKFDGALPDGASKNG
                     GNMEEFVLPAGIVLTGFTPSFMPVVGFIEDVGETKDNKTEPRRYPRDYWKGITPAGYG
                     ATAWFPARIAVTGPADYTLNSVGVCTSNTVHNGWRTQVWETDHPVKILNVICGHWKER
                     RGHGTTIYYAPVHPYNIDAMSATLDAARKYYSEWFLPYPWKELKLSEFPGMAGYAQGF
                     GTNITFSENIGFLTRNDAKTNATFLVTAHESAHQWWGNIVTPARGPGGDFLSEAMAHF
                     STMLLFDKVLGPRERMEFSKGLEARYGDRRRVDDERPMYDVDGKRESDETVIYDRGGW
                     VFWMLYDYLGHDRALEGYRAFCRTWSVSRDHPALQDIIAAMRPYAADPVKYDAFVKEW
                     FEDKAMPEYRLANASKRKIGSGYDITAVVKNVGTGTMPVTVAATAGERWKSATPKTAT
                     GPGTAQAAPLDDKASGALGDAAKAMSGAGASSEQDPRYREARNTVTLGAGESRTVTIH
                     CDFAPEKLVVDPDVQVLQLRRKQAVAKL"
     gene            complement(7964..>8686)
                     /locus_tag="VFM00_06705"
     CDS             complement(7964..>8686)
                     /locus_tag="VFM00_06705"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET7497972.1"
                     /translation="NATCASCHGLAGPRLTNAHKGFSLAGKNCVSCHDPHVQPVGKKG
                     LLQPALHAPFAKGDCAKCHGTATTGALAQRVPDLCYSCHGNTKAWSSKPVVHAPLKAD
                     ASCVSCHAPHGGPATPVLKAEQSKLCFQCHDRKMVTGKFKHAALDEGCTACHDPHSSD
                     RKKLLVNDVNTLCRGCHEDMSKHFHPTTGGKDPRTGDPLSCIGCHNPHSSAEDHLLTH
                     EPKRALCIQCHDPSMQKMLEKK"
BASE COUNT         1434 a         2949 c         2938 g         1365 t
ORIGIN      
        1 agacgggcca ggagggccgg gattcgtagc ggaaggggaa gccatagcgc tcggcggcga
       61 cgcgcgtgac gttcggcttg aggaagatgt gcgtgtcggg atcggcgagg aagccgaaca
      121 cggtcacgat cggccaggtg aggacgcgcg tctgcttgcg gggaagatcg gcgaccacgt
      181 cgcgccatcg ctcgaaacgc cgctcgatgg ccccacggcc gtgcaggaat tcgtacagac
      241 cctccgcgaa ggcccgggcg ccggccacag gcttcacggc atcgcgcagc gccatctttt
      301 cgaacgagaa gaggagattc gttcgcgact cgatggtcac ggcgcgggat gccacctcat
      361 ggaacgcccc ctgcaggatc aacccgcgcc agacggtggc gggcagaagc tccatccagc
      421 gctggtgcgc ctcccacttg tagttccgct cccactcgag atacgtctcg tcccggaacc
      481 cgcccggaaa cgcgcgcagg aacttgcggc ggcgtgccgc gacccggggc gcgcgtgagg
      541 cgttgtccct gggacgggcg gcacggtcaa tgcgcggaat tgccatggtc cactcctctc
      601 tcggagcgaa cccagcaatg tccgggccgg attggggcgg gtagcttggg aaatggagcc
      661 ctggcccgct gccatggcgg ggctccgagg aggtcgacgg aatcaaccgc cctgtccacc
      721 ttgccctcag gaaatcagac cgtctggaag atctcccgct tgccgctgac ccacatctcc
      781 gtgtagccct tcatcttttc gcgcaggtcc gtgcgctcct catattcctt catgcgcgcg
      841 gtgagagcgg caaggtcctc gacctcggtt tccatgacca ccgtgttgaa cgtccccacg
      901 gcgtcggtca tgattcgcgt cttccacttg ggctgcgcgg ccatgacttc cttcatgtgc
      961 ttggccagct tcgaggccat tccgggcttt gccacgaacg attcacgcac gatcagcatg
     1021 gtcggtctcc ttggcgaggg aaggtacggc aactcgcccc ggggcgagtt gcccggggca
     1081 aacgtgcagc gtgacgcggc gcgacgatcg cgcaggcgtc aatcgcgcgc gacatccgtc
     1141 attcaatttc accggcgctg gcgcaggtaa cataaatgtt aagtgcgccg tgatcctgcc
     1201 ccagtacgca tccccaggca gggtgtgaca gcggttcgct acggagaagt tgaccccacc
     1261 gcgacgagct gtccgttctg cagcgcgagc acggggcagt agagcccgag ctgctcccgg
     1321 cgccaccatg cgccggtccg cttcctttcc tccgcggtcg tgaagtgata gtcgtagacc
     1381 accgcccgca ggtacttcgg aggcgcgcgc gggaacggat cggtcgcgaa gagcgcggcg
     1441 acgggctggg aaccctccag cagacgccgg cacagctgca ggaaccacgg ctggttctgg
     1501 aaattgctga gcgccgcgaa ccacatctgc cagtccagcc gcggctgatg aggcgcgacg
     1561 aactcggggc gacgcgccgg atcgcccggc ttccagcgaa gctcgtacgg caaccatcgg
     1621 accccatcgg cgctcccctc gagcacgatc tccagcctcc gcgtggtcat gaccgcgaag
     1681 agcccgtagc gattgaacga tcgaacgggc cagaccaggt cggacacata ctccaccggg
     1741 ccgagccacg ccgtgggcgc gtgcagcgtg ctcaagaacg gcaccaggct gagcaggaag
     1801 aaaacgacgg ccagcggccg gatcgcccag ccgaaccagc tcccacggcg gttcgcgctc
     1861 catcgcgcgg tccgctcgcg ccatcccctc ggccagacgc cgtcgtccag gcacagcacg
     1921 cagagcgcca gcgtgagcca gttgaagaag ccgtagtttc cggtgaccaa gatcaggatc
     1981 tgaagcgatg cgatgagacc gacgcccacg aagcggatcc ggcgcggcag gaagaccagg
     2041 aatggaacga tcccctcgag gatcagcgtt cccgtgctgg aagcccgcag cagtcccgcg
     2101 gacaggtgat gggcgaacca tgcggtccag ggcggaagcg gctgcgtctc gaagtgatat
     2161 tgcagcgcgg tgcgggcatg ccatgagggg tcgccgctca agagcttcac ggccgccgag
     2221 gagaaggaaa gtcggaagag gagccatcgc atcagccaga ggcccgatcg gcgcggcggg
     2281 gcatcgttcc aaggccgcga ccatccccgc catggcgcga tcaggatcgc gaggagcccg
     2341 gtctcgagca gcaggctgtc ccactggaac cagaagaagt cctgccccag gatcgagagc
     2401 gacagatacg tgagccagag acccgccagg caggcgatcg gtgcggcgcc gagcacgagc
     2461 cccacggaga gcgcaacgcc cgcggcgcac atcgcgttca ggaatcccgc gctcgtcccc
     2521 agccagccaa gcgtcggtgc gatccagaag gccagccgtc cgtacttggc atgaagcgcg
     2581 gccaggaagt cgcgcgcggg gaggatcccg tccgggccgg cgatcccctg cacctggacc
     2641 cacatcgaga cgaacgcgac gagatacacg acggcgagga ggcggaggaa gatccaggcc
     2701 gtgagcgtct cgccgggggg ggcgacgtga tcgccccaga tccagcgggt gacacggtac
     2761 agcggcccgc ggttcgcggc gacgagccga tacgcggctt cggtgagggg ccggacgccc
     2821 ggaacccgcc ggtagagcca taacggccat cgccggccgg gcgccgtcgc ctgggcgcgg
     2881 aaaaccgcct cggcgcctcc ggaccacttc ccatccggct cccgaagctg gacggccgcg
     2941 gcgaaccgct cacggggaac ctcggggaag cggtcggcga cttcctgata cgtcgcgtac
     3001 tcgacacggt cgcccgtcgt ggcccgccac cgctcgatcc aaagccggca gaaaccacag
     3061 tcgccgtcgt agaccagaag gggtttcatt cccctcattt ccccctcatt ccccctcatt
     3121 ccaaagccct ccagagcccg cccgtgacca gcccggggga gtatcctaga atctccggcg
     3181 ggaaacccta tccagaatca aggcgtagag aacgcgtcgc cgatccgcca gcacactccc
     3241 aggaggcccc gatgctcagc acgcgcggtc tggtcaaggt gtacccgggc ccggtcaccg
     3301 ccttgaacgg cgtggatctg gacgtgccgc ccgggatgtt cggcctcctc ggtcccaacg
     3361 gggcggggaa gtcgaccttc atgaagatcc tcgccgggct cctcgagccc acctccggca
     3421 ccgccacact cgacggcatc gatatcgtga aggaccccga ctcggtctcg ccgcggctgg
     3481 gctacctgcc gcaggaattc ggcttctatc cccacctgac gggcgaggcg atgctcgggc
     3541 atcttctgga tctcaagggg gtgagcgcgc cgcaggggaa gaagaagctc gtcgccgagc
     3601 tgctcgaccg cgtgaacctg tcgttcgcgg ccaggcgcgc ggtgaagggt tactcgggcg
     3661 gcatgcggca gcgcctcggg atcgcgcagg cgatcgcggg gaatccgcgg ctgatcatcg
     3721 tggacgagcc gacggcggga ctcgaccccg aagagcggct ccgcttctat catctcctct
     3781 cggagctcgc ggcggaccgc atcgtgctcc tctcgacgca catcgtcgag gacgttgccg
     3841 tcctctgccc gcgcttcgcc gtgatccgca cggggcggct gatcgccctg accacgcccg
     3901 ccgaagcgcg ggccgccatc gcggggcgca tgttcgaagg gctggtctcg gtggaggatc
     3961 tgccgcggct tcgcgcggag cggatggtga ctcaggcgct cctcgtggag gggaagaacc
     4021 gcgcccgcat cttcgagccc gcgggcacgc cgcccccggg gttcgtaccg gctccggcca
     4081 cgctcgagga tgcctatctc gtcctgatgc gccaggggga gctgccgaac ggaaacggct
     4141 cgagtccggc accggcttcc ggccgcgtcg cgggaacgaa cgccgccatc gaggcgattg
     4201 gatgagcccg cgccgcctgt tcgccgtctt cttagtcgag ttcggccatt ccttcaagcg
     4261 gccgctcttc tacatcatgg cgttcctcat cgccctcatc tccttcgggc tctcgagcgg
     4321 ccagatgcag atctcctccg gcgacagctc ggtgggaggg accaaggcct ggatcacctc
     4381 cgagttcgcc cagacgcaga cgatgaccta cctgacgatc ctcatcttcg cggggttcat
     4441 cgcggccgcg gcgggcatga cgctgctcca cgatcgcgag accaaggtgg acgtgatcct
     4501 ccactccacg ccgctcaagg cgggagagta cgtctggggg cggctcctgg ccgtggtcgg
     4561 gatgttcgcg gtcctgctcg cctggctggc gctgaccgcc gccttcttca accacctcgt
     4621 gccgaatgga aacgcgctgg agatccgcgg ccccttcgcg tgggccagct acttccggcc
     4681 ggtcctgttc gtggggcttc cgttcgtggt gttcttcgcg gggctttcga tgtggatcgg
     4741 cgagcggacc cggagcgccg tgatcgtcta tcttcttccc gccctcgcca tcctggtcag
     4801 cggcttcttt ctcatgacgt ggtccccatc gtggctttcc tacgggatga accgcgtgct
     4861 gcaggtggtc gagccgtcgg gctatcgctg gctcaacgag acgctgctca aggtggatcg
     4921 cggcgtgaag tactacaaca cgcaaccggt cccctacgac gcgatcttct ggctgaaccg
     4981 gctctggatg ctgttcgtgg ggtttggcgg catcctcctc acgcagcgct cgatcgcgag
     5041 gtcgcggcgg ggcgtcgaga gcgcacgcgc gcgcacgaag agcgccgcgg cgcggccgga
     5101 agcgctcggc gccggctggg acgagacggc cgttcgtccc tcgatccagg ggatcgggat
     5161 gacgagccgc gttccggggg cgctcgccac ggtggcggcc gtggcgcgga ccgagacgaa
     5221 ggagctcttg cgccagcccg cgctctacct cttcatgctg atcatcctgg tccaggtgct
     5281 ggggaactcg ctgctcgcgg tgggcgcgtt cgacactccg gtcctcctga cgccgggcac
     5341 gagcgcggtg gccctcgcca atcagagcgt caccttcatc tgcctcctcc tcatgtacta
     5401 caccgtggta tcgctggaac gggagcacac gaccggcctg gcgccggttc tctacgcggc
     5461 tccggtgcgg accggagcgc tcctcttcgg aaaagcgctg gcgaacggcg tggtcggggc
     5521 gatcgtcctt gtggcctccc tcgtcggaag ctggctcgcc atctcgatcc agcacaccgt
     5581 tccgttctcg ctgggcccgt acctcctggt ctgggggctg ctcctggtgc cgaccttcat
     5641 gttctggacg gccctcgtca cggcggtcta cgccctcgtc gggaaccgct acacgacgat
     5701 cgcgatcgcc ctgggcatcc tggcctggac cggctggcgc gcgctgacca agcagatcag
     5761 ctgggcgggc aactgggcgc tctggggcgc gctccgctgg agcgacctcg gccccttcca
     5821 gcacgaccgc ctggtgctcg tcttgaaccg cgcgatggtg ctgagcgcgg ccttcctctt
     5881 catcgccctc gccgtgcggc tcttccgccg ccggagcccc gacgcggtgc gcacgatcca
     5941 ccgcctgtcg cccaggagcc tccgtcgctc cgcgttccgt ctcgcgccct acgcgttgat
     6001 cccggcggcc ctctggatca ccctcctctt ccaggtgagc cagggcacgg aagggggggc
     6061 cgcgaagaag gcgcgcaagg actactgggc gaagaatctc aagacctgga tggacgcgcc
     6121 ccttccggac atcgcgcgcg ccgatatcgc cgtgaaggtc gatccccagg ggaaccggct
     6181 cgagagccgc ggggccctga cgttcgtgaa cggtctggac acgacgctga cccagatccc
     6241 gttgaccggc gggaccgaat ggaagaacgt gacctggacc atgaacggac gcgccgtcac
     6301 gccggaggac tcgaagcggc tctacgtctt cacgccgccg cgtccgctcg ccaaggggga
     6361 cagcgtcgtc atcgggtgga agttcgacgg ggcgctgccg gacggcgcca gcaagaacgg
     6421 cgggaacatg gaggagttcg tgcttcctgc cggcatcgtg ctgaccggct tcacgccgag
     6481 cttcatgccg gtggtcggct tcatcgagga cgtcggcgag accaaggaca acaagactga
     6541 gccgcgccgc tacccgcgcg actactggaa ggggatcacc cccgcgggct acggcgcgac
     6601 ggcctggttc ccggcgcgca tcgccgtgac cggcccggcc gactacacgc tcaattcggt
     6661 cggcgtctgc acgagcaaca cggttcacaa cgggtggcgc acgcaggtct gggagaccga
     6721 ccacccggtc aagatcctga acgtgatctg cgggcactgg aaggagcggc gcggccacgg
     6781 cacgacgatc tactacgcgc cggtgcaccc ctacaacatc gacgcgatgt cggccacgct
     6841 ggacgccgcg cggaagtact actccgagtg gttcctgccc tatccctgga aggagctcaa
     6901 actctccgag ttcccgggca tggccggcta cgcgcaggga ttcggcacga acatcacctt
     6961 cagcgagaac atcggcttcc tcacgcggaa cgacgccaag accaacgcga cgttcctggt
     7021 cacggcgcac gagtcggcgc accaatggtg gggaaacatc gtgacgcccg cccgggggcc
     7081 cggaggggac ttcctgagcg aggcgatggc gcacttctcc acgatgctcc tcttcgacaa
     7141 ggtgctcggc ccgcgcgagc ggatggagtt ctcgaagggg ctggaagcgc gctacggcga
     7201 ccgccggcgc gtggacgacg agcggccgat gtacgacgtg gacggcaagc gcgagtcgga
     7261 cgagacggtg atctacgacc gcggcggctg ggtcttctgg atgctgtatg actacctggg
     7321 gcacgatcgc gcgctcgaag gataccgggc cttctgccgc acctggagcg tgagccgcga
     7381 ccatcctgcg ctccaggaca tcatcgccgc gatgcggccc tacgcggccg atcccgtgaa
     7441 gtacgacgcc ttcgtgaagg aatggttcga ggacaaggcg atgccggagt accgcctggc
     7501 caacgcctcg aagcgcaaaa tcggctcggg ctacgacatc acggcggtgg tgaagaacgt
     7561 cggcacggga acgatgccgg tgacggtcgc ggcgacggcg ggcgagcggt ggaagagcgc
     7621 gactcccaag acggccaccg ggccgggtac ggcccaggcc gccccgctcg acgacaaggc
     7681 gtcgggagcg ctgggcgacg cggcgaaggc gatgtcggga gcgggcgcca gctcggagca
     7741 ggacccccgc taccgcgagg cgcggaacac ggtcacgctc ggggcggggg agtcgcggac
     7801 ggtgaccatc cattgcgact tcgcgcccga gaagctggtc gtggatcccg acgtccaggt
     7861 gctccagctc cgccggaagc aggcggtcgc gaagctgtag cgcctcccgc ccaaaacgaa
     7921 gccgcggagc cggctctcgc cggatccgcg gccacattcc tcgctacttc ttctccagca
     7981 tcttctgcat ggacgggtcg tggcactgga tgcacagcgc ccgcttcggc tcgtgcgtca
     8041 ggagatggtc ctcggcgctt gagtgcgggt tgtggcatcc gatgcagctc agcggatcgc
     8101 cggtccgcgg atccttgccg cccgtggtcg gatggaagtg cttgctcatg tcctcgtggc
     8161 acccgcggca gagcgtgttc acgtcgttca cgagcagctt cttccggtcc gaagagtgcg
     8221 gatcgtggca ggcggtgcac ccctcgtcga gcgccgcgtg cttgaacttg cccgtgacca
     8281 tcttccggtc atggcactgg aagcagagct tgctctgctc ggccttcagc accggggtgg
     8341 ccggcccgcc gtgcggcgcg tggcaggaga cgcacgaggc gtcggccttg agcggggcat
     8401 ggacgaccgg cttgctcgac cacgctttcg tgttcccgtg gcaggagtag cagagatccg
     8461 gaacgcgctg cgcgagcgcg ccggttgtgg ccgtgccgtg acacttggcg cagtctccct
     8521 tggcgaaggg ggcgtggagc gccggctgca gcagcccctt cttcccgacc ggctgcacgt
     8581 gcggatcgtg gcaggagacg cagttcttcc cggcgagcga gaagcccttg tgcgcgttgg
     8641 tcagccgcgg cccggccagc ccatggcatg acgcgcaggt cgcgtt
//
LOCUS       DASSLZ010000173         5652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1063024, whole genome shotgun sequence.
ACCESSION   DASSLZ010000173 DASSLZ010000000
VERSION     DASSLZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063024"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..710
                     /locus_tag="VFM00_06710"
     CDS             153..710
                     /locus_tag="VFM00_06710"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET7497973.1"
                     /translation="MVSRPGGTLGLNVALIGVSGAGKTTVAPLAAARLGFPWVDLDAA
                     IVAASGRTAASFFEAGEEAAFRVLEREALERALAGRRPGVILACGGGLVTAPETRALL
                     RARAFVVWLKVSHARALARLGSGGIAERPLLAGDAPEALERLLRERAPLYEGAADAVI
                     DTDGLTPDDVAERVAALCRPDPTWP"
     gene            739..1302
                     /gene="rsmD"
                     /locus_tag="VFM00_06715"
     CDS             739..1302
                     /gene="rsmD"
                     /locus_tag="VFM00_06715"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET7497974.1"
                     /translation="MGLIRVVGGALRGRRIAVPDRGVRPTSERTREAIFDLLGPDRVP
                     GARVLDLYAGTGALGIEALSRGAASADFVEKDPAAARRLTETLAKLGLSDVARVHRAD
                     LDGGELPEGAVGPWEIVFLDPPYAGGAGAKWLAALAEGGWEAGEGLVIHERARATPAA
                     APEGLSLWKERTYGDTTVAIYRRGGGV"
     gene            1299..1784
                     /gene="coaD"
                     /locus_tag="VFM00_06720"
     CDS             1299..1784
                     /gene="coaD"
                     /locus_tag="VFM00_06720"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET7497975.1"
                     /translation="MKSAIFAGTFDPVTNGHVDLIRRALRLVDRLTVAVAARPEKKVL
                     FTQEERVLMIREAVPDEPRLAVEPFTGLLVQYAEARGIGTIIRGLRFVSDFEYEFQMA
                     LMNRRLSGDIETLFLMPSEAYTFVNSSLVKEIARHGGDVSPFVPDSVRSRLIAALGGK
                     R"
     gene            1781..3001
                     /locus_tag="VFM00_06725"
     CDS             1781..3001
                     /locus_tag="VFM00_06725"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7497976.1"
                     /translation="MSRGPTAETGTARHRVSARVRALKPSETLALSARARELRARGER
                     VVSFAAGEPDFDTPVHIQDAAVEAIRAGHHRYTDVGGVPALRNAIAEKLRKDNGVTYG
                     PREIVVSNGAKHSIWNALFSLLEPGDEVLIPVPYWVSFPEMVRLSGGEPVFVTPARGG
                     LKVSAEDLDRAATPRSRALILNSPNNPSGAVYAREEIEAIADLVRRRDLMVVSDEIYE
                     RLVYGAARHVSIASFGEEARARTVLVNGVSKTWAMTGWRIGYAAAPAEIAEAMERLQG
                     QSTSNPSSVSQQAALKAITGDDAPAQAMKARFEARRDLAVERIGRMKGVRLAPPEGAF
                     YVFPDFSERIAAARNGVATSAQLCDYLIDEAKVVCVPGAAFGMEGHVRISYAVSEKDL
                     EEGLDRIAAALERM"
     gene            3029..3340
                     /locus_tag="VFM00_06730"
     CDS             3029..3340
                     /locus_tag="VFM00_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HET7497977.1"
                     /translation="MPTYEYECEKCGHRFEVFQSMSDPPKKRCPKCRSKVRRLIGTGA
                     GMIFKGSGFYVTDYRSDSYKEQKKKESGDSSHAKDQPKPKSDAGSKAKKKPKAGGTSD
                     S"
     gene            3510..4595
                     /locus_tag="VFM00_06735"
     CDS             3510..4595
                     /locus_tag="VFM00_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HET7497978.1"
                     /translation="MAYRVTLIPGDGTGPELAAATRRVLEATGVRFDWDIQDAGIDVH
                     KKEGTPLPARVLDSLRKNGVGLKGPITTPVGTGFRSVNVALRKELDLYACERPCKTYP
                     GVRSRYDNVDIVIIRENTEDLYAGIEFEMGTPGALALRAVVKLQLQREISEDAGISIK
                     PISAHGSARIVRHAFEYARKNGRKQVTAVHKANIMKFSDGLFLAEARRIAGEYPDIEF
                     NDRIVDNMCMQLVQVPENYDVLVLPNLYGDIISDLAAGLVGGLGVAPGANIGDKAALF
                     EPTHGSAPKYTGKDKVNPLAMILSGMLMLRYLGEKEAGERLERAIARVIHDGQRVTYD
                     MKADRMDPTAVGTQAAADAVIQALDAD"
     gene            4613..>5652
                     /gene="obgE"
                     /locus_tag="VFM00_06740"
     CDS             4613..>5652
                     /gene="obgE"
                     /locus_tag="VFM00_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET7497979.1"
                     /translation="MLADRARIEVIAGRGGNGSVSFRREKYVPKGGPDGGDGGHGGSV
                     ILRANPHLRTLLDFQSRTRFEAQNGAHGSGNQKSGRSGEDLIVEVPTGTLVVDDGTGD
                     RLADLVEAGAEFVAARGGRGGRGNARFATPTRQAPREAQPGREGEERRLRLELRLIAD
                     VGLVGFPNVGKSTLLARVTAARPKIADYPFTTLEPHLGLVRVGEERSFVMADLPGLIE
                     GAHAGKGLGHEFLRHIWRTRVLAILVDSLSPDPARELAALRHELSSYHEDLARKPAVV
                     VLSRSDLSPSASTAEQPAPFPLHGARWGGSISGVTGAGTRDLLEIIWTMLAGGDTLPP
                     EGRERDDTVAKR"
BASE COUNT          867 a         1901 c         2098 g          786 t
ORIGIN      
        1 acgacgaacg ccaagcgcga gctcctgatc ttcgtgacgc ccaagatcct gggcgaccag
       61 accgcttcga cgaagtagcc gatccgcgcc tcttccgcgc gcggcgggac gccggtatac
      121 tgcggggcct tcctcggaag gccccgtttt tcatggtgtc ccgtccggga ggaaccctgg
      181 gtctcaacgt ggccttgatc ggagtctcgg gagcgggcaa gaccacggtg gcgcccctcg
      241 cggccgcgcg gctcggattc ccctgggtgg acctggacgc cgcgatcgtg gccgcgagcg
      301 gccggaccgc cgcctccttc ttcgaagcgg gggaagaggc ggcgttccgc gtgctcgagc
      361 gcgaagcgct ggagcgcgcg ctggcgggcc ggaggccggg cgtgatcctg gcctgcggcg
      421 gcggcctcgt gacggcgccg gagacgcgcg cgctgctccg ggcgcgggcg ttcgtcgtgt
      481 ggctcaaggt cagccacgcg cgcgcgctgg cgcgcctggg aagcggcggc atcgcggagc
      541 ggcccctcct tgcgggagac gctcccgaag ccctggagcg gctcctccgg gagcgcgcgc
      601 cgctctacga gggcgcggcg gacgcggtga tcgacaccga cgggctcacg cccgacgacg
      661 tggccgagcg ggtggcggcc ctctgccggc ccgatcccac gtggccgtga gccggcgggc
      721 cctgagcgag cggacggcgt ggggctgatc cgcgtcgtcg gcggggcgct ccggggacgc
      781 cggatcgccg tgcccgaccg cggcgtgcgg ccgacgtcgg aacggacccg cgaggcgatc
      841 ttcgacctcc tgggaccgga tcgggtaccc ggcgcgcgcg tgctcgacct ctacgcgggg
      901 acgggcgcgc tcgggatcga ggccctctcg cgaggggcgg cttcggcgga cttcgtcgag
      961 aaggaccccg cggcggcgcg gcggcttacc gagacgctcg ccaagctcgg gctctccgac
     1021 gtggcgcgcg tccatcgcgc cgacctcgac ggcggggagc tgcccgaggg cgcggtgggg
     1081 ccctgggaga tcgtcttcct ggatccgccc tacgcgggcg gggccggggc gaaatggctc
     1141 gcggcgctcg cggagggggg ctgggaggcc ggcgaggggc tggtgatcca cgagcgcgcc
     1201 cgcgcgaccc cggccgcggc gcccgagggg ctctcgctct ggaaggagcg gacctacggc
     1261 gacacgacgg tcgcgatcta ccggagggga ggaggcgtat gaagagcgcg attttcgcgg
     1321 ggacgttcga ccccgtcacg aacgggcacg tggacctgat ccggcgggcg cttcgcctgg
     1381 tggaccgcct gaccgtggcg gtggcggcgc gtccggagaa gaaggtgctc ttcacccagg
     1441 aggagcgggt cctcatgatc cgcgaggccg tgccggacga gccgcgcctg gcggtggagc
     1501 cgttcaccgg gctgctcgtc cagtacgccg aggcgcgcgg catcggcacc atcatccgcg
     1561 ggctccgctt cgtctccgat ttcgagtatg agttccagat ggccctgatg aaccggcggc
     1621 tgagcgggga catcgagacc cttttcctga tgccgagcga ggcctacacg ttcgtgaatt
     1681 ccagcctggt gaaggagatc gcccgccacg gcggcgacgt ctcgccgttc gtccccgact
     1741 ccgtccgctc gcgcctgatc gcggcgctgg gagggaagcg gtgagccgcg gcccgacggc
     1801 cgagaccggc acggcccgcc atcgcgtgag cgcgcgcgtg cgcgccctga agccctcgga
     1861 gacgctcgcc ctctcggcgc gcgcccgcga gctccgggcg cgcggcgagc gcgtggtctc
     1921 gttcgccgcg ggcgagcccg acttcgacac cccggtccac atccaggatg ccgccgtgga
     1981 ggcgatccgc gcggggcacc accgctacac cgacgtcgga ggcgtccccg cgctccggaa
     2041 cgcgatcgcg gagaagctcc ggaaggacaa cggcgtgacg tacggaccgc gcgagatcgt
     2101 ggtgtcgaac ggggcgaagc actcgatctg gaacgcgctc ttctcgctgc tcgagccggg
     2161 agacgaggtg ctgatccccg tgccgtactg ggtcagcttc ccggagatgg tccgcctgtc
     2221 gggcggcgag ccggtgttcg tcacgccggc gcgggggggc ctcaaggtat ccgccgagga
     2281 tctcgaccgg gccgcgacgc cgcggtcccg cgcgctcatc ctgaacagtc cgaacaatcc
     2341 ctcgggcgcg gtgtacgccc gggaggagat cgaggcgatc gccgacctcg tccggaggcg
     2401 cgacctcatg gtcgtctccg acgagatcta cgagcggctc gtctacggcg cggcgcgcca
     2461 cgtgtcgatc gcatcgttcg gggaggaggc gcgggcgcgc accgtgctcg tgaacggagt
     2521 ctccaagacg tgggccatga ccggctggcg gatcggctac gccgcggcgc cggccgagat
     2581 cgccgaggcg atggagcggc tccaggggca gtccacctcg aacccctcgt cggtctcgca
     2641 gcaggccgcg ctcaaggcga tcaccggcga tgacgctccc gcccaggcga tgaaggcgcg
     2701 cttcgaggcg cgtcgcgacc tggcggtcga gcggatcggc cggatgaagg gggtccgtct
     2761 ggctccgccg gaaggggcgt tctacgtctt tcccgatttc tcggagcgga tcgccgccgc
     2821 gcggaacggg gtcgcgacct cggcgcagct ctgcgattac ctgatcgacg aggccaaggt
     2881 ggtctgcgtt ccgggcgcgg cgttcggcat ggagggacac gtccggatct cgtacgcggt
     2941 ctccgagaag gatctcgagg aagggctcga ccggatcgcc gccgcgctgg agcggatgta
     3001 ggcaccccac gggagggaag ggtcttcgat gccgacctac gaatacgagt gtgagaagtg
     3061 cgggcaccgg ttcgaggtgt tccagtcgat gtcggacccg cccaagaagc ggtgcccgaa
     3121 atgccgcagc aaggtccgcc gtctgatcgg aaccggcgcg gggatgatct tcaagggctc
     3181 cgggttctac gtcaccgatt accgctccga ctcctataag gaacagaaga agaaggagtc
     3241 cggcgattcg tctcacgcga aggatcaacc caagccgaag agcgacgcgg gctcgaaggc
     3301 caagaagaag cccaaggcgg gcggaacttc cgattcctga gccgtttccc tttgacgcgc
     3361 ccgggccccg gtgctagcgt gccggtatcg cgcccggcca ctcgattccc cctcggacgg
     3421 ccgccgctgt gttcggttca cctcgacgca agtaaaagaa cgacaatctg tttcggcgtg
     3481 aacctccacc tttttgcaag agggagcgca tggcttaccg cgtgacgctg attccggggg
     3541 acgggaccgg tcccgagctg gccgcggcga cgcggcgggt gctcgaggcg accggcgtcc
     3601 gattcgattg ggacatccag gacgccggca tcgacgtcca caagaaggaa ggcacgccgc
     3661 ttccggcccg cgtgctcgac tcgctccgca agaacggcgt cggactcaag ggaccgatca
     3721 ccacacccgt cgggaccggg ttccgcagcg tgaacgtcgc gctccggaag gagctcgatc
     3781 tctacgcgtg cgagcgcccc tgcaagacct atccgggcgt gcggtcgcgg tacgacaacg
     3841 tggacatcgt catcatccgc gagaacaccg aagacctcta cgccgggatc gagttcgaga
     3901 tggggacgcc cggcgccctg gcactgcgcg ccgtcgtgaa gctgcagctg cagcgcgaga
     3961 tctcggagga cgcggggatc tcgatcaagc cgatcagcgc gcacggatcg gcgcggatcg
     4021 tgcgccacgc cttcgagtac gcgcggaaga acggacgcaa gcaggtcacc gcggtgcaca
     4081 aggcgaacat catgaagttc tccgacggcc tcttcctcgc ggaggcgcgc cgcatcgcgg
     4141 gggaataccc cgacatcgag ttcaacgacc gcatcgtcga caacatgtgc atgcagctcg
     4201 tgcaggtccc cgagaactac gacgtcctcg tactgcccaa tctctacggt gacatcatct
     4261 ccgacctggc cgcgggcctg gtgggcggat tgggcgtggc ccccggcgcc aacatcggcg
     4321 acaaggcggc gctcttcgag ccgacgcacg gcagcgcgcc caagtacacc ggcaaggaca
     4381 aggtgaatcc gctcgccatg atcctgagcg ggatgctgat gctgcgctac ctgggcgaga
     4441 aggaagcggg cgagcgtctg gagcgcgcca tcgcgcgcgt catccacgac gggcagcgcg
     4501 tgacctacga catgaaggcc gaccgcatgg atccgacggc cgtcgggacg caggccgcgg
     4561 cggacgcggt gatccaagcg ctcgacgcgg actaagggaa cgaacggacg cgatgctcgc
     4621 ggatcgcgcc cgcatcgagg tgatcgccgg ccggggcggc aacggctcgg tcagctttcg
     4681 gcgcgagaag tacgtgccga aggggggacc ggacggcggg gacggcgggc atggcggcag
     4741 cgtgatcctg cgcgcgaacc cgcatctgcg cacgctgctc gatttccagt cgcgaacgcg
     4801 gttcgaggcg cagaacggcg cgcacgggag cggcaaccag aagtcgggac gctccggcga
     4861 ggacctgatc gtcgaggtgc ccacgggaac gctcgtggtg gacgacggga ccggagaccg
     4921 gctcgccgac ctggtcgaag cgggcgcgga gttcgtcgcg gcgcggggcg gacgcggcgg
     4981 ccgcggcaac gcgcggttcg cgacgcccac gcggcaggcg ccgcgggagg cgcagcccgg
     5041 gcgggagggc gaggagcgcc ggctccgctt ggagctgcgc ctcatcgcgg acgtcgggct
     5101 ggtcgggttt ccgaacgtcg gcaagtcgac gctcttggcg cgcgtcaccg cggcgcgccc
     5161 aaagatcgcc gactatccgt tcacgacgct cgagccgcac ctggggctcg tacgcgtcgg
     5221 cgaggagcgc agcttcgtga tggccgatct gccgggcctg atcgaagggg cgcacgcagg
     5281 gaagggtttg gggcacgagt tcctccgcca catctggcgg acgcgcgtgc tcgcgattct
     5341 ggtcgacagt ctttcgcccg atccggcccg cgaactggcc gcgctccggc acgagctctc
     5401 ctcgtatcac gaggacctcg cccggaagcc ggcggtggtc gtgctcagcc ggtcggacct
     5461 cagtccgtcg gcgtcgacgg cggagcagcc ggcgccgttt ccgctccacg gagcgcgttg
     5521 gggagggtcg atttccggcg tcacgggcgc cggcacgcgg gatctgctcg agatcatctg
     5581 gacgatgctc gccggcggcg acaccctgcc gcccgaggga cgggaacgcg acgacaccgt
     5641 cgcgaagcgc aa
//
LOCUS       DASSLZ010000174         7787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1580287, whole genome shotgun sequence.
ACCESSION   DASSLZ010000174 DASSLZ010000000
VERSION     DASSLZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580287"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..803)
                     /locus_tag="VFM00_06745"
     CDS             complement(93..803)
                     /locus_tag="VFM00_06745"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET7497980.1"
                     /translation="MPSPSRLDELMREGEEALDAGEFDAALERFEAVLAGDPEHWGAA
                     LDRAETLHLLWRTREALRAVTALRPPEDEEDDADRADLEARIREVTGDFGDADRLFAE
                     AHRLDPEHFPEPVRMNAEDFRSVLDEVLAALPEPIRNAVEEVPVVVQPKPTPAMAEYA
                     PHITPEILGLFVGTSLGEKVSAGSGYADVVLLFQKNLEHAGTTAEEVAEEIRITLLHE
                     YGHYLGFDEDGIEDLGLA"
     gene            complement(813..1262)
                     /locus_tag="VFM00_06750"
     CDS             complement(813..1262)
                     /locus_tag="VFM00_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497981.1"
                     /translation="MRRAPFTVLVAALAALAIGAVSCQTLAGKGDAGADFAAYRTALS
                     EAKGRMDRAAQDSDVQGVSKAMKEIGDQFNAIESKTSSMNLMDNETMKIQIATGRNTI
                     TQTGPWVQNNDAEAVRAQVAQLDPVLSEIDVLLDRAVKSSAPASGTP"
     gene            complement(1259..2035)
                     /locus_tag="VFM00_06755"
     CDS             complement(1259..2035)
                     /locus_tag="VFM00_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET7497982.1"
                     /translation="MAIAMEHHSHDPVPSKWPIITALGAGLIPIGIIASTRGVKAGIA
                     ILLFGLLVTILGAGRWWAELLRDSFVGRDLLDADARLRTAMILFILSEAAIFASFFAA
                     IIYARTHAKSWPPEGMPHFGRLLPAVNTGILLSSSVTCHLAHVAIERKNREGVVSALL
                     LTIFLGAIFLCGQAYEYGVLNGEAFTIKSGIFGSTFFMLTGFHGLHVLMGIVFLNVVY
                     GAVMRGRVTHEQQFPLKAASWYWHFVDAIWIGVFTIVYLL"
     gene            complement(2178..2891)
                     /locus_tag="VFM00_06760"
     CDS             complement(2178..2891)
                     /locus_tag="VFM00_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7497983.1"
                     /translation="MPRLLGRVRAALCLALCFTVAPIAASTSHAQTAGLAVTLLWTAT
                     GDNGLVGQAAKYDLRYTASTITGTDTLSWWNKAAVVNMTGHVPPPSGARDSVVIAGLV
                     LGKKYFALLRVADAAGNWSGFSNMATIDLTKNISGVDVAELGAPKLVVGAPYPSPTAG
                     TAQIALTLARSGPLSAEVFDARGRLVRSLHTGAMEAGPHTLRWDGSAESGQQASAGVY
                     WIRVAAEGMQKSVKLVVIH"
     gene            complement(3155..6364)
                     /locus_tag="VFM00_06765"
     CDS             complement(3155..6364)
                     /locus_tag="VFM00_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7497984.1"
                     /translation="MKLSEISIQRPVLATVMSLAIILFGVLSFTRLPVREYPDVDPPI
                     VSITTFYRGASPSVVETEITDVLEEQLATLEGVKTIESSSKEQGSSISVEFELSRNIE
                     EAANDVRDKVSRVRGQLPSEADDPIVEKVDVNAQPIVWLALSSKRFSNLELSDVADRV
                     LKERLQRLPGVGNIFIGGERRYAMRVWLDPARMAAHGLTTQDVEAAVRRENAEIPAGR
                     VEGTEREFAVRTRGDLVTAEEFGAIIVKQDGTETVRLDDVADVKVGAEDERTAVRWNS
                     QPAVGLGVVKQSKASTIDVADAVRAALPQLQASVPEGMKLDVAYDSSTFIKDSIHEVS
                     QTIFIAIILVILVILFFLKTVRATLIPGVAIPISIVGAFAAAYFMGFTINILTLLALV
                     LAIGLVVDDAIVMLENVYRHMEMGKGRMRAAIDGSKEIGFAILATTITLVAVFVPVSF
                     LTGTVGRLFSEFGLSVAVAVLISGFVALTLTPMLCSRILKSSHDKDQGRVASRIDRFM
                     DGVNTRFAGSLRWFLEHRGVALGIAALLLVLIGGMFFLLPRELVPTEDRSIGFGVVIA
                     PEGSTLEYTDRYMRQVEGILMPLPERRGLFTATGLGFGGPGQVTNGFLFLGLKSPGER
                     PKSWVKTPLPIFPMIQVPVYRARSQQEIVQQLFPQLISIPGVLAFVINPPSIGGRFSS
                     SPVEYVLQGEDYDQLAQGVGAMMGEASKLGYLLNLDTDLRLNKPQLDVSIDRERAAQL
                     GVSVTDIGSTLETLLGGRTVSEFKRGTKQYDVIAQVSPQNRSTPGTIDDIYIRGTGGL
                     VQLANVVTIKETVAPKELNHYNRVRSATITANLPPGLPLGMALDALDKIAAEKLPAGM
                     KHELAGQSREFRESSTSLYFLFGLAVVFIYLVLAAQFESFVHPLTILFAVPLAVAGAL
                     VSLFIFRQSLNIYSQIGLIMLIGLVTKNSILIVEYANQLRARGLSIVDAVAQAAQIRL
                     RPILMTSLATIFGILPIAIGLGAGAEARRPLGIAVVGGMIFSTFLTLVLVPVVYTYLA
                     RFTKVATHGTPEGAAPEEAPAPAHEEEPVPAMATSRTRV"
     gene            complement(6361..7548)
                     /locus_tag="VFM00_06770"
     CDS             complement(6361..7548)
                     /locus_tag="VFM00_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7497985.1"
                     /translation="MTRRSASDGSRALATAAALLILTAAGCQKGPKGGGFQMPPMPVE
                     TAPVSQGAVADRFEAVGSVEAGEAIQVVSEIDAVVKRLPFAEGQPVRAGALLMQLDDA
                     QLKADVSRTEALRDQAKASYSRVKAVVGQGAGAPQDLDDAAAALKVAEANLSLAESRL
                     RKTRIMAPFAGVTGPKRVSPGAYVRAGTPITDLASIGVIKVTFSVPERYLGRLHRGAP
                     VTLSAPAFPGYTISGAIDVLDPVLDPQTRSSRVIARVRNPGGRFRPGMSVNVAAVLAQ
                     RPEAVTVPSEAVFSEGGENFVYVVKKDSTVTKSSIQLGTRLADVVEVVKGLAPGMIVV
                     RTGHQKLFENAKVIPIPPGGPGGPGGPGGASGPGGASGPAGAGAGGKTTAGKVPASKK
                     GTS"
     gene            complement(7545..>7787)
                     /locus_tag="VFM00_06775"
     CDS             complement(7545..>7787)
                     /locus_tag="VFM00_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497986.1"
                     /translation="RLDAARRGVAASIEQVRIGVLEYNYGRTTAFELSRLSADLAAAQ
                     QRYSQALVRTARAAAQLRYLTSGGAPPATPRGEGTE"
BASE COUNT         1272 a         2590 c         2718 g         1207 t
ORIGIN      
        1 atggccagca ggggttgcat gaaagagaag tatagcggga ccgggcgcct ccggcagccc
       61 gcggcgaggg agaaaccccc gcgccttcgg cgctacgcca ggcccaggtc ctcgatgccg
      121 tcctcgtcga agccgaggta atggccgtac tcgtggagga gcgtgatgcg gatctcctcc
      181 gcgacctcct cggcggtcgt cccggcatgc tcgaggttct tctggaagag gagcacgacg
      241 tcggcgtatc ccgatcccgc gctcaccttc tcgccgagcg aagtccccac gaagagtccc
      301 aggatctcgg gcgtgatgtg gggcgcgtat tcggccatgg cgggcgtggg cttgggctgc
      361 acgacgacgg gcacttcttc caccgcgttc cggatcggct ccggaagcgc ggcgagcacc
      421 tcgtccagga cggagcggaa gtcctcggcg ttcatgcgca ccggctcggg aaagtgctcg
      481 ggatccagac ggtgcgcctc cgcgaagaga cggtccgcgt cgccgaagtc tccggtgacc
      541 tcgcgaatcc gcgcctcgag atcggcgcgg tcggcgtcgt cttcctcgtc ctcgggagga
      601 cggagcgccg tgacggcgcg cagagcctcg cgcgtgcgcc agagaaggtg aagcgtttcg
      661 gcgcggtcca gggccgcgcc ccagtgctcg gggtcgccgg cgaggacggc ctcgaagcgt
      721 tccagcgcgg cgtcgaactc acccgcgtcg agcgcctctt ccccctcgcg catcaactcg
      781 tccagacgcg agggagaggg catggcgtca gcctatggcg ttcccgaggc gggcgcggag
      841 gactttacgg cgcggtcgag caggacgtcg atctcggaga ggacgggatc gagctgggcc
      901 acctgggcac ggaccgcctc ggcgtcattg ttctgcaccc aggggccggt ctgcgtgatg
      961 gtgttgcgtc ccgtggcgat ctggatcttc atcgtctcgt tgtccatcag gttcatcgac
     1021 gaggtcttgg actcgatggc gttgaactga tcgccgatct ccttcatcgc cttggaaacg
     1081 ccctggacgt cggagtcctg cgccgcgcgg tccatgcgcc ccttcgcctc gctcagcgcc
     1141 gtgcggtagg cggcgaagtc cgcgcccgcg tcccccttcc ccgccagcgt ctggcaggag
     1201 acggccccga tggcgagagc cgcgagagcc gcgacgagaa ccgtgaaagg agcgcgcctc
     1261 acaggaggta cacgatcgtg aagacgccga tccagatcgc gtccacgaag tgccagtacc
     1321 aggaggccgc tttcagcggg aactgctgct cgtgcgtcac gcggccgcgc atcaccgcgc
     1381 cgtagaccac gttcaagaag acgatcccca tcagtacgtg cagcccgtgg aagccggtga
     1441 gcatgaagaa ggtcgagccg aagatgccgc tcttgatcgt gaacgcctcg ccgttcagca
     1501 cgccgtactc ataggcctgt ccgcagagga agatggcgcc caggaagatc gtgagcaaga
     1561 gcgccgagac caccccctcg cggttcttcc gctcgatcgc gacgtgcgcc agatggcagg
     1621 tgaccgacga ggagagaagg atgccggtgt tcacggccgg cagcagtcgg ccgaagtgcg
     1681 gcatgccctc cggcggccag ctcttcgcgt gggtgcgcgc gtagatgatc gcggcgaaga
     1741 acgaggcgaa gatcgccgcc tccgagagga tgaagaggat catcgccgtc cggagccgcg
     1801 cgtcggcgtc cagcaggtcg cgccccacga agctgtcccg gagcagctcg gcccaccagc
     1861 gccccgcgcc caggatcgtg acgaggagtc cgaacaggag gatcgcgatc cccgccttca
     1921 cccctcgcgt cgaggcgatg atgccgatcg ggatgagccc cgccccgagg gccgtgatga
     1981 tcggccactt gctggggacc gggtcgtggg aatggtgctc catcgcgatc gccatgcgcg
     2041 ctcctccgga atcgttacgg gtcggaattc agcgggcgag tatgccttcg cggacgggcc
     2101 atcgcaagcg ccttgtgtcc cgcgaacgga gggcgacgct cgaaaggaaa cctgctggag
     2161 gggggaaccc ggggggatca gtggatgacg acgagcttca cgctcttctg catgccctcg
     2221 gcggcgacgc ggatccagta gacgccggcg gaggcctgct ggccgctctc ggcggagccg
     2281 tcccagcgca gcgtgtgcgg gcccgcttcc atggcgccgg tgtggagcga gcgaacgagc
     2341 cggccccgcg cgtcgaagac ctccgccgac aggggcccgg aacgggccag cgtgagcgcg
     2401 atctgcgcgg ttccggcggt gggagacgga tagggcgcgc cgaccacgag tttcggcgcg
     2461 cccagctccg cgacatccac cccgctgatg ttcttggtga gatcgatggt ggccatgttg
     2521 gagaagccgc tccagttccc cgcggcgtcg gcgacgcgga gcagcgcgaa gtacttcttt
     2581 cccagcacga ggcccgcgat cacgacggag tcccgggcgc ccgacggagg cgggacgtgt
     2641 ccggtcatgt tcacgaccgc cgccttgttc caccacgaga gcgtgtcggt gccggtgatc
     2701 gtgctggccg tatagcggag atcgtacttg gcggcctggc cgacgaggcc gttgtctccg
     2761 gtcgcggtcc agagaagggt gacagcgaga cccgcggtct gcgcgtgcga cgtggatgcg
     2821 gcgatgggcg ctacggtgaa gcagagggcg agacacaacg cggcacggac gcgtccgagc
     2881 aggcggggca tgatccttcc ctgggtgcga gttcgagatg tgacggatcg gatgtcgggt
     2941 tcggaactgc ggtctcaaga ggcaggaaac gtgccgcgaa gacgcgaccg acggggaggc
     3001 gcgcagcgag ggaagatacg cgggtcgaaa cggctcacga gtcgacgagg tggacgcggc
     3061 gtgtaccctc gcgtgcgcgc ggcgtcccga gaaggaacgg ccctatctgc cgatactata
     3121 aagacgtttt agagtcgccc tctccgcggc gccgtcagac gcgcgtgcgg ctcgtggcca
     3181 tggccgggac cggctcttcc tcatgggcgg gagcgggcgc ctcttccggc gcggcaccct
     3241 ccggagtccc gtgggtggcc accttcgtga agcgggccag gtaggtgtag accaccggca
     3301 ccagcaccag cgtgaggaac gtggagaaga tcatcccgcc caccaccgcg atcccgagcg
     3361 ggcgccgggc ctccgcgccc gcgccgagcc cgatcgcgat cgggaggatg ccgaagatcg
     3421 tcgccagcga cgtcatgagg atggggcgaa gccggatctg cgccgcctgc gcgaccgcgt
     3481 ccacgatcga gagcccgcgc gcccgaagct ggttggcgta ctccacgatc aggatcgagt
     3541 tcttggtcac gagaccgatc agcatgatga gcccgatctg ggagtagatg ttgaggctct
     3601 gccggaagat gaagagcgac accagggccc ccgcgacggc gagcggcacg gcgaacagga
     3661 tcgtcagcgg atggacgaag ctctcgaact gcgccgcgag caccaggtag atgaacacga
     3721 ccgcgagccc gaacaggaag tagaggctgg tgctggactc gcggaactcg cgggactgcc
     3781 ccgccagctc gtgcttcatg ccggccggca gcttctcggc ggcgatcttg tcgagcgcgt
     3841 ccagtgccat gcccagcggc agcccggggg gcaagttggc cgtgatcgtg gccgagcgga
     3901 cccggttgta gtggttgagc tccttgggcg cgaccgtctc cttgatcgtg accacgttcg
     3961 caagctgcac caggccgccg gtcccgcgga tgtagatgtc gtcaatggtg cccggggtgg
     4021 atcggttctg cggcgacacc tgcgcgatca cgtcgtactg cttcgtcccg cgcttgaact
     4081 cgctgaccgt gcgaccgccc aagagcgtct cgagcgtgct tccgatgtcc gtgaccgaga
     4141 cgccgagctg ggccgcgcgc tcgcgatcga tcgagacgtc gagctgcggc ttgttcaggc
     4201 gcaggtcggt gtccaggttc aggagatacc cgagcttgga cgcctccccc atcatggccc
     4261 cgacgccctg cgcgagctga tcgtagtctt ctccctggag cacgtactcg acgggcgatg
     4321 aggagaagcg tcccccgatc gagggcgggt tgatgacgaa cgcgagcacg cccgggatgg
     4381 agatgagctg ggggaagagc tgctgcacga tctcctgctg cgaccgcgcg cgatacacgg
     4441 ggacctggat catgggaaaa atcggaagcg gcgtcttgac ccacgacttc ggccgctcgc
     4501 cgggcgattt gagccccagg aacaggaatc cgttggtgac ctgccccggt ccgccgaacc
     4561 cgagccccgt cgccgtgaac agcccgcgcc gctccggcaa gggcatgagg atgccttcca
     4621 cctggcgcat gtagcggtcg gtgtattcga gcgtggatcc ctcgggggcg atcacgaccc
     4681 cgaatccgat gctccgatcc tcggtgggga ccagctcgcg cgggaggagg aagaacatcc
     4741 ctccgatcag aacgagcaga agcgccgcga tgcccagcgc cacgccgcgg tgctccagga
     4801 accagcggag cgacccggcg aaccgcgtgt tcacgccatc catgaagcgg tcgatgcggg
     4861 aagccacgcg tccctgatcc ttgtcgtggg aggacttgag gatccgggag cagagcatcg
     4921 gcgtgagcgt gagcgccacg aatcccgaga tgagcacggc gaccgcgacc gagaggccga
     4981 attcgctgaa cagccgccct accgttccgg tgaggaatga gaccgggacg aacaccgcga
     5041 ccagcgtgat cgtggtggcg agaatggcga agccgatctc cttcgagccg tcgatcgcgg
     5101 cgcgcatgcg cccctttccc atctccatgt gccggtagac gttctccagc atcacgatcg
     5161 cgtcgtccac gacgagcccg atcgcgagaa ccagtgcgag cagcgtcagg atgttgatcg
     5221 tgaatcccat gaagtaggcg gccgcgaagg cgcccacgat cgagattggg atcgcgacgc
     5281 ccgggatcag cgtcgcccgc acggtcttca ggaagaaaag gatcaccagg atgacgagga
     5341 tgatcgcgat gaagatcgtc tgcgacacct cgtggatgga atccttgatg aacgtcgagg
     5401 agtcgtaggc gacgtccagc ttcatcccct cgggcaccga ggcctggagc tgcggcagcg
     5461 cggcgcgcac ggcgtcggcg acgtcgatgg tgctcgcctt ggactgcttc accacgccga
     5521 ggcccacggc gggctggctg ttccagcgca cggcggtccg ctcgtcttcg gcgccgacct
     5581 tgacatcggc cacgtcgtcc agccgtaccg tctcggtgcc gtcctgcttc acgatgatcg
     5641 cgccgaactc ctcggccgtg accagatcgc cgcgggtccg caccgcgaac tcgcgctcgg
     5701 taccctcgac gcgtcccgcc gggatctcgg cgttctcacg gcgcacggcg gcctcgacgt
     5761 cctgggtcgt gagcccgtgg gcggccatgc gagcgggatc gagccagacg cgcatggcgt
     5821 agcggcgctc gccgccgatg aagatgttgc ccacgccggg aaggcgctgg aggcgctcct
     5881 tgagaacccg gtcggcgaca tcggacagct ccagattact gaatcgcttg ctggacaggg
     5941 cgagccacac gatcggctgc gcgttcacgt ccaccttctc gacgatggga tcgtccgcct
     6001 cgctcggcag ctgcccgcgt acgcgggaga ccttgtcgcg aacgtcgttc gcggcctcct
     6061 cgatgttgcg ggagagctcg aactcgaccg agatcgagga gccctgctcc ttgctggagg
     6121 actcgatcgt cttcacccct tccagggtgg cgagctgctc ttcgagcacg tcggtgatct
     6181 cggtctcgac cacgctcggg ctggcgccgc gatagaacgt ggtgatcgag acgatcggcg
     6241 ggtcaacgtc cggatattcg cgcaccggga gccgtgtgaa ggagagcacc ccgaagagga
     6301 tgatcgccag gctcatgacc gtggcgagga ccggccgctg gatggagatt tcgctcagct
     6361 tcatgaggtt ccctttttgg atgccggaac cttgccggcc gtcgtcttgc cgccggcgcc
     6421 cgctccggcg gggccgctcg ctccgcccgg accgctcgct ccgcccggac cgcccggacc
     6481 gcccggacca cccggcggga tcggtatcac tttagcgttt tcgaacagct tctggtgccc
     6541 ggtgcgcacg acgatcatcc ccggcgccaa tcccttgacc acctcgacga cgtcggcgag
     6601 ccgcgttccc agctggatcg agctcttggt gaccgtgctg tccttcttca cgacgtagac
     6661 gaagttctcg cccccttccg agaagaccgc ctcgctcggc acggtgacgg cctcggggcg
     6721 ctgcgccaac acggcggcca cgttgaccga catccctggg cggaagcgcc cgcccgggtt
     6781 ccggacccgg gcgatcacgc ggctcgagcg cgtctgcgga tcgagcaccg gatccagcac
     6841 gtcgatggct cccgagatgg tgtaccccgg aaacgccggc gccgagaggg tcaccggcgc
     6901 gccgcggtgc aggcgaccca ggtagcgctc gggcacggag aaggtcactt tgatcactcc
     6961 gatcgaggcc aggtcggtga tcggggttcc cgcgcgcacg taggcgcccg ggctcacgcg
     7021 cttcgggccc gtcacgcccg cgaagggcgc catgatgcgc gtcttccgga gtcgcgactc
     7081 ggccagggag aggttcgcct cggccacctt gagcgccgcg gcggcgtcgt ccagatcctg
     7141 cggcgcgccg gcgccctgcc ccacgaccgc cttcacgcgc gagtaactcg ccttcgcctg
     7201 gtcgcggagt gcctccgtgc gggagacgtc cgccttgagc tgtgcgtcgt ccagttgcat
     7261 gaggagcgct ccggcgcgga ccggctgccc ctcggcgaag gggagccgct tcacgacggc
     7321 gtcgatttcc gagaccacct ggatcgcctc gcccgcctcc accgacccca cggcttcgaa
     7381 acggtcggcc acggccccct gcgacaccgg ggcggtctcg accggcatcg gcggcatctg
     7441 gaagccgcct cccttcgggc ccttctggca gccggcggcg gtcaggatga gaagcgcggc
     7501 cgcggtcgcg agcgcgcgcg aaccatcgga agcggagcgt cgggtcattc ggttccttcc
     7561 ccccgtggcg tcgccggcgg ggctccgccg gacgtgagat aacgaagctg tgccgcggcc
     7621 cgggccgtac gcaccagggc ctgagagtag cgctgctgcg cggcggccag atcggcggag
     7681 aggcgcgaca gctcgaaggc cgtcgtcctt ccgtagttgt attcgaggac gccgatccgc
     7741 acctgctcga tggaagcggc cacgccgcgg cgcgcggcat cgagccg
//
LOCUS       DASSLZ010000175         7312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1830517, whole genome shotgun sequence.
ACCESSION   DASSLZ010000175 DASSLZ010000000
VERSION     DASSLZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830517"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VFM00_06780"
     CDS             complement(<1..997)
                     /locus_tag="VFM00_06780"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HET7497987.1"
                     /translation="MTKKETLVLEPIQGGTVVPRSESGNALVIALLVLMVLTSAGVAY
                     VAVTKSEKQIAGNTMTSSQAMYAAEAGIAEGLHRMAFPAESLNYIGPPTLPYAGWGKY
                     IVLTNGASLLDPNRAALASDGLDNDGDGFVDESGEAYPEVLTKQTVNANALVYPYVRV
                     EYKVQGNQLVRFGDADEDPTTPPVENLQYGAPVLRITASGRRGNAAKILEAEAVRFPI
                     ISAKGPLWAGGLMNFNGNAFLIDGHDHYAASPNDTIPGAAPAPAVLTEGGTSDATLAV
                     NQQDNVTGDGGDNSVQHSTYTYDFNQIWTVLAGMADYSLTGPLTFGSSSPSYGSYA"
     gene            complement(994..3222)
                     /locus_tag="VFM00_06785"
     CDS             complement(994..3222)
                     /locus_tag="VFM00_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET7497988.1"
                     /translation="MNHKRDQADAGFTLIEITIAVLLFALVIGSIYQVYVRGERSQQV
                     GLELAEANQNARSGVDLIARELRSAGYGINPAVQPSIVVGSQYRVTFALDVNGNRQID
                     VGEVITYFLDPSSSDPLVASSPNPYDFVLRRKVGTAADSLATPVSGEGEIVAYGLTQR
                     SADNITAKNVPLFSYRDNGGVALELKAGTSNDAAGVFYGKTVTNTDLGIPPGPGTSSR
                     VKTVLVNMVTETKQKNVQTGGYDRVSIGTSVEPRNFPFLAQANLAYAPANTGGTGGTG
                     YGTGTGSGSGTGSGSGTGSGSGTGTGTGTGTGLPPSQPPIHIPTDRVLSLALADLDEY
                     DRLEGSATTQNDQHDLDIIVGTRASGTANIRVWWNGQPGRYTGNVYYQATESYLGNAS
                     YDIQALAAADLDGSGNNTRDVVSGVLTGTNVGRFQVWLNQCFGGASEIRGRVGTTTSP
                     VTPNTQYYDNPGTGEVKGLALGDLNSDGRIDVVIGTKTGSNVGKVEVWWGAGNGTFTH
                     SALLDVYTASGEVRSLSIRDMNLDGYPDIVAGTKTNTSDTQGNVDIYFNNALSIVRFT
                     TVYTVAVGGAVYAITTNRMDADSYPDVVTAVKNGTTTGKIEFWHNNGTMAGALTRQDE
                     QATPGPALSLAVGRLDWGNVSNDIVVGTAGAGGATPPAIQAFFCDPSASGGAVIPNVY
                     SWADANAGGAVNALAIGKLECSQDTPDNDPLADIVAGTATSASTGDLVIYLNPYASTV
                     LP"
     gene            complement(3256..3633)
                     /locus_tag="VFM00_06790"
     CDS             complement(3256..3633)
                     /locus_tag="VFM00_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497989.1"
                     /translation="MSRRPVRAGAAPGFSLIEVLFALTFLAVGILAVASMIPAGTKGV
                     TQSRVITSSLMAAQIKMEQLRGTPYQSLTSGTFTDTTSVFTRTWVVTDSIPMAGCKRI
                     QVTSGWINSQGAQSTVLTSYVTR"
     gene            complement(3630..4130)
                     /locus_tag="VFM00_06795"
     CDS             complement(3630..4130)
                     /locus_tag="VFM00_06795"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HET7497990.1"
                     /translation="MKGGLAAPAARDAGAGFTLVELAIVLVIFGIVSAIAIPGLNKFL
                     RSVEMNGEVQQFATTMRVVRQRAITENNNYVLYWDATDRKLKWWDDDNNNGVQDGPEK
                     FGETSAMANWITVTNSSTNPFPSDTVLFVPNGSASVSGSVIYSNPEGYTRSLSVVRPT
                     GMVTVQ"
     gene            complement(4328..5101)
                     /locus_tag="VFM00_06800"
     CDS             complement(4328..5101)
                     /locus_tag="VFM00_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET7497991.1"
                     /translation="MTAHALLSTAAFLLGLVFGSFLNVVIARLPRGQSIVSPPSSCPR
                     CKARIRPWDNIPVLSFLLLRGRCRSCRKPISWRYPAVELTAGILLGQLAQRAEHPAVL
                     VAQAVFVLALVAVAWIDFDTRTIPDVVTIPGVGVGLAASLFGPPGLGSALMGAAAGGL
                     SLWLVGAAYERATGVPGMGGGDVKLAAMMGAFLGAGGVFGAIFLASFAGSLFGVLLIA
                     RGKGDKRSAIPFGTFLAPSAVALLLYGDALFRWYRSLLP"
     gene            complement(5098..5775)
                     /locus_tag="VFM00_06805"
     CDS             complement(5098..5775)
                     /locus_tag="VFM00_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497992.1"
                     /translation="MTAGLPTPLRAAFPFAAAAALLAGSALLYAASWSRIGPAPGEEL
                     LYFPSGVFVRQASLGFETAAADAAWLRAIQYYGEHRLTDQKFQRIDHVMAVVTDLDPA
                     FESAYVFGAFVLAQELRRPEEGLALLTKGRHANPESWRLAFETGFLHYVCRHDYAAAS
                     SCFVQASRLPGHPDYVERFAAFASQRSGNRDLAVLLWKRVLATGNRYMQEVAKRELAR
                     LEAPEAL"
     gene            complement(5772..6539)
                     /locus_tag="VFM00_06810"
     CDS             complement(5772..6539)
                     /locus_tag="VFM00_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497993.1"
                     /translation="MTRVAAIAKNTLREVARERVFLVLVLFGLGLAGVSLVLAPLALG
                     EGSKVVLDFGLAGASALSTLVVIVLGSSLLHKELDRRTIYAVMAKPIRRSEFLFGKYA
                     GLWIGAAALLATMIALTAVLLLLSGGAFPWTVGGALLLSLAELMIVTGVVVFFSSFTT
                     PLLTAFFTAATLLAGHFAEDLLYFGTHGASRLLAALTQGAYWLLPHLEVFNARGLVVH
                     GVAVGQDRLLFAAAYAALYGGALLAAAAAVFERRDFK"
     gene            complement(6536..>7312)
                     /locus_tag="VFM00_06815"
     CDS             complement(6536..>7312)
                     /locus_tag="VFM00_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7497994.1"
                     /translation="ILLGLSRPTAGVVRIEGRDPRDPEARRRLGYLPENPCFYDHLTA
                     TEYLDLVGSLFGMPREERRARGMELLERLGLGQHASKALRTFSKGMTQRLGLAQALLN
                     RPSLLVLDEPMSGLDPVGRAEVKRLLRDERGRGATVLLSSHVLAETEALCDRVGILAS
                     GRLVETGSVPGLLAGGVREWEIAAERIPERVAVTLRAAGHAAETVGSRWVVRVADAAA
                     LQEVLRTLLAHDVTVHAVEPRRPTLEEHFMAALGRAEASR"
BASE COUNT         1160 a         2492 c         2553 g         1107 t
ORIGIN      
        1 tggcgtacga cccgtaggac ggggagctgg atccgaacgt cagcggcccc gtaagggagt
       61 agtcggccat cccggcgagt accgtccaga tctggttgaa gtcgtacgta taggtggaat
      121 gctggacgct gttgtcgccg ccgtcgcccg ttacgttgtc ctgctggttc accgccagcg
      181 tcgcgtcgct cgtgcctccc tcggtgagca ccgcgggggc cggagccgct ccggggatcg
      241 tatcgttcgg cgacgccgcg taatgatcat gcccgtcgat caggaacgcg ttgccgttga
      301 aattcatgag gccaccggcc cacagcggac ccttggccga gatgatcggg aacctcaccg
      361 cctcggcctc gaggatcttc gccgcgttgc cgcgccgtcc cgaagcggtg atccggagga
      421 ccggcgcgcc atattgcaga ttttcgactg gcggcgtcgt cggatcctcg tcggcatccc
      481 caaaccgcac gagctgattg ccctgcacct tgtactctac ccgcacgtag gggtagacca
      541 gggcgttcgc gttcaccgtc tgcttggtca gcacttcggg gtaggcctct cccgactcgt
      601 ccacgaaacc gtcgccgtcg ttgtccagcc cgtccgacgc aagcgccgcg cgattggggt
      661 ccagaaggct cgcaccgttg gtgagcacga tgtacttgcc ccagccagcg tagggcaggg
      721 tcggcggtcc gatgtagttg agcgactcgg ccggaaaggc catgcgatgg agtccctcgg
      781 cgattcccgc ctccgcggca tacatcgcct ggctcgacgt catcgtgttc cccgcgatct
      841 gtttctccga cttggtcacc gccacgtagg cgacgccggc cgaagtcagg accatgagca
      901 cgaggagcgc gatgacgagc gcgttccccg actcggagcg aggcacgacc gttcctccct
      961 ggatcggctc gagcacgagg gtctccttct tcgtcacgga agcaccgtgg acgcgtaggg
     1021 gttcaggtag atgaccaggt cgcccgtgct cgcgctggtc gcggttccgg ccacgatatc
     1081 ggcgagcgga tcgttgtccg gggtgtcctg cgagcactcc agcttaccga tcgcaagggc
     1141 gttcaccgcc ccgccggcgt tggcgtcggc ccacgagtac acgttgggaa tgaccgctcc
     1201 gcccgaggcg ctggggtcgc agaagaaggc ctggatggcc gggggcgtcg caccgcccgc
     1261 gcccgcggtc ccgaccacga tgtcgttgct cacattgccc cagtcgagcc ggccgaccgc
     1321 gagcgagagc gccgggccgg gcgtggcctg ttcgtcctgg cgcgtgaggg caccggccat
     1381 cgtcccgttg ttgtgccaga actcaatctt gcctgtggtc gtgccgtttt tcaccgccgt
     1441 cacgacgtca ggataggagt ccgcgtccat gcggttcgtc gtaatcgcgt acaccgcgcc
     1501 gccgacggcc acggtgtaga cggtcgtgaa gcggacgatc gagagggcat tgttgaagta
     1561 gatgtcgacg ttcccctgcg tgtccgacgt gttcgtcttg gtgcccgcca cgatgtcggg
     1621 gtagccgtcc aggttcatgt cgcggatcga aaggctccgc acctccccgc tcgccgtgta
     1681 gacgtcgagc aaggcgctgt gcgtgaacgt cccgttgccg gcgccccacc agacctcgac
     1741 cttgcccacg ttggagccgg tcttggtgcc gatgacgacg tcgatgcgcc cgtcgctgtt
     1801 caggtcgccc agcgccagcc ccttcacctc gcccgtgccg ggattgtcgt agtactgcgt
     1861 gttgggcgtg accgggctgg tcgtcgttcc cacacgaccg cggatctcac tggcaccccc
     1921 gaaacactgg ttcagccaca cctgaaagcg gcccacgttg gtgccggtga ggacgccgga
     1981 gacgacgtcc cgggtgttgt tccccgatcc gtcgaggtct gctgcggcaa gggcctggat
     2041 gtcgtagctc gcgttgccca ggtagctctc cgtggcctgg tagtagacgt tgccggtata
     2101 acggccgggc tgtccgttcc accacacgcg gatgttcgcc gttccgctcg cgcgggttcc
     2161 gacgatgatg tccaggtcgt gctgatcgtt ctgggtggtc gccgagccct cgagccggtc
     2221 atattcgtcc agatcggcca gcgccagcga gagcacgcgg tcggttggga tgtggatcgg
     2281 cggctggctg ggcggaagac ccgtgccggt gccggtcccc gtgccggtgc cgctgccgct
     2341 ccccgtgccg gagccgctgc ccgtgccgga gccggagccg gtgccggtgc cgtatccagt
     2401 tccgcccgtg ccgccggtgt tggccggcgc gtaggccagg ttcgcctggg cgaggaacgg
     2461 gaagttgcgc ggctcgaccg aggtgccgat gctcacgcga tcgtagccgc cggtctgcac
     2521 gttcttctgc ttggtttccg tcaccatgtt gaccaggacg gtcttcacgc ggctggacgt
     2581 gccgggcccc ggcggaatac ccaggtcggt gttggtcacg gtcttcccgt agaagacgcc
     2641 cgcggcgtcg ttcgacgtgc ccgccttgag ctccagcgcc acgccgccgt tgtcccggta
     2701 ggagaagagg ggaacgttct tggccgtgat gttgtccgcc gagcgctggg tcaggccgta
     2761 ggccacgatc tcgccctcac ccgagaccgg cgtggccagc gagtcggccg cggtgcccac
     2821 cttgcggcgg agcacgaagt cgtaggggtt cgggctggac gccacgagcg ggtcgctcga
     2881 gctgggatcc aggaagtagg tgatcacctc gcccacgtcg atctgccggt tgccgttcac
     2941 gtcgagcgcg aaggtgaccc gatactggga gcccaccacg atcgacggct gcacggcggg
     3001 attgatgccg taccccgccg agcgcagctc gcgcgcgatc agatccacgc cgctgcgcgc
     3061 gttctggttc gcctccgcca gctcgagccc gacctgctgg gagcgctcgc cgcggacata
     3121 gacctgatag atcgacccga tcaccagcgc gaacagcagc acggcgatcg tgatctcgat
     3181 gagggtgaag cccgcgtcgg cctgatctcg tttgtggttc acggtatcac ctcgggtgcg
     3241 gggctccctg cccggtcaac gggtcacgta gctggtcagg acggtgctct gtgcgccctg
     3301 gctgttgatc cagcccgacg tgacctggat gcgcttgcat cccgccatgg ggatggagtc
     3361 cgtcacgacc caggtccgcg tgaagacgga ggtcgtgtcc gtgaaggttc cggacgtgag
     3421 actctggtac ggcgtgccgc gaagctgctc catcttgatc tgggccgcca tgagcgagct
     3481 cgtgatgacg cggctctggg tgacgccctt cgtcccggcc gggatcatcg acgcgacggc
     3541 gaggatgccg accgcgagga acgtcagcgc gaagagcacc tcgatcaagc tgaagccggg
     3601 cgcggcgccg gcgcggacgg ggcggcggct cactggaccg tcaccattcc ggtggggcga
     3661 acgacggaga gcgaccgggt gtacccctcg ggattcgagt agatgacgct ccccgaaacg
     3721 ctggcgctcc cgttgggcac gaacaggacc gtgtccgacg ggaacggatt tgtggaggaa
     3781 ttggtcaccg tgatccagtt cgccatggcc gacgtctctc cgaacttctc gggaccgtcc
     3841 tggactccgt tgttgttgtc gtcgtcccac catttgagct tccgatccgt cgcgtcccag
     3901 tagaggacgt agttgttgtt ctccgtgatc gcgcgctgcc gcaccacccg catcgtggtc
     3961 gcgaactgct gtacttcgcc gttcatctcc acggagcgca ggaacttgtt gaggccggga
     4021 atcgcgatcg ccgacacgat gccgaagatc acgagcacga tcgccagctc gaccagcgtg
     4081 aagccggcgc cggcgtcgcg cgcggcaggg gccgcaaggc cgcctttcat cgtccgttcc
     4141 tccttggaat gcgtgtcgtg ccggtgctca acaacgcaac gaccatgcca gcgcccaaac
     4201 cgcgttccgg ccgtcgtctg gccagcgctt cgaggctcga tgcggcggat ttgccccgtc
     4261 gctggcgcga tcgagccatg accgcgcgac ggcgccgggc gcgccttccg tgcaatccgc
     4321 acgcgtctca agggaggagc gagcggtacc agcggaagag cgcgtccccg tagagaagga
     4381 gcgccacggc cgagggcgcg aggaaggttc cgaacggaat ggcgctgcgc ttgtcgccct
     4441 tcccgcgcgc gatcaggagc accccgaaca acgacccggc gaacgacgcc aggaagatcg
     4501 cgccgaacac cccgccggcg cccaggaacg cgcccatcat cgcggcgagc ttcacgtcgc
     4561 cgccccccat gcccggcaca ccggtcgcgc gctcgtaggc cgcgcccacc agccagaggc
     4621 tcaagcctcc cgccgccgcg cccatcaagg cggagcccag tcccggcgga ccgaagaggc
     4681 tcgcggccag gccgaccccg acgcccggaa tggtcacgac gtcggggatc gtgcgcgtgt
     4741 cgaagtcgat ccacgcgacc gcgaccagcg cgagaacgaa caccgcctgg gcgacgagca
     4801 ccgcgggatg ctccgcgcgc tgcgccagct gcccgagaag gatccccgcc gtgagctcca
     4861 ccgccgggta gcgccaggag atcggcttcc ggcaggaacg gcaccggccc cgaagaagga
     4921 ggaacgatag gacggggatg ttgtcccagg gccggatccg ggccttgcag cgcggacacg
     4981 acgacggcgg cgaaacgatc gactggccgc gcggaaggcg cgcgatgacg acgttcagga
     5041 aggagccgaa caccaggccc agcaggaacg ctgcggtcga gaggagcgcg tgcgcggtca
     5101 cagggcctcc ggcgcttcga ggcgggcgag ctcgcgcttc gccacctcct gcatgtagcg
     5161 gttccccgtc gcgagcacgc gcttccagag gagcaccgcg aggtcgcggt tcccggagcg
     5221 ctgcgaggcg aacgccgcga agcgctcgac gtagtcgggg tgacccggca gccgcgacgc
     5281 ctgcacgaag cagctcgacg cggcggcata gtcatggcgg cagacgtagt ggaggaagcc
     5341 ggtctcgaac gcgaggcgcc acgactccgg gttggcgtgc cgccccttcg tgaggagggc
     5401 cagcccctct tccgggcggc gcagctcctg cgccagcacg aaggcgccga agacgtacgc
     5461 cgactcgaac gcggggtcca ggtcggtgac gaccgccatc acgtgatcga tgcgctggaa
     5521 cttctgatcg gtcaggcgat gctcgccgta gtactggatc gcgcgcagcc aggccgcgtc
     5581 ggccgcggcg gtctcgaacc cgagcgaggc ctgcctcacg aacacaccgg aagggaaata
     5641 gagcagctct tccccgggcg ccggtccgat gcggctccac gacgcggcgt agaggagcgc
     5701 ggacccggcg agcagcgcgg ccgccgccgc gaaggggaac gccgcccgca ggggggtcgg
     5761 caggcccgcg gtcacttgaa gtcccgccgc tcgaagaccg cggccgccgc ggcgaggagc
     5821 gcgccgccgt agagggccgc gtacgccgcc gcgaagagga gccgatcctg cccgaccgcg
     5881 acgccgtgaa cgaccaggcc gcgcgcgttg aacacttcca ggtgcgggag cagccagtac
     5941 gcgccctggg tcagggcggc gagcaggcgc gaggcgccgt gcgtcccgaa gtagaggaga
     6001 tcctcggcga aatgaccggc gaggagcgtg gccgcggtga agaaggccgt gagcagcggc
     6061 gtcgtgaacg aggagaagaa gacgacgact cccgtcacga tcatcagctc ggccagggac
     6121 aggaggagcg cgccgccgac cgtccaggga aaggcgccgc ccgacagaag gaggagcacc
     6181 gcggtgaggg cgatcatcgt ggcgagcagc gccgcggcgc cgatccagag cccggcatac
     6241 ttgccgaaga ggaattccga acggcggatc ggcttggcca tgacggcgta gatcgtccgg
     6301 cggtccagct ccttgtggag gaggctcgag cccagcacga tcacgacgag cgtggagagc
     6361 gccgaggcgc cggcgagccc gaagtcgaga accaccttgc tcccctcgcc cagggcgagc
     6421 ggcgcgagca cgagcgagac cccggcgagg cccagcccga agaggaccag cacgaggaag
     6481 acccgctccc gcgccacctc gcgcagggtg ttcttggcga tcgcggcgac gcgggtcatc
     6541 gggaggcctc cgcgcggccc agggcggcca tgaaatgctc ctccagggtg gggcgacgcg
     6601 gctccacggc gtgcaccgtc acgtcgtgcg ccagcagcgt ccgcaggacc tcctggaggg
     6661 cggccgcgtc ggcgacgcgc acgacccacc gcgagcccac cgtctccgcc gcgtgcccgg
     6721 cggcgcgcag cgtcacggcg acccgttccg ggatccgttc cgccgcgatc tcccactccc
     6781 gaacccctcc ggcaaggagt cccggcaccg agccggtctc gacgagacgc cccgaggcca
     6841 ggatgcccac gcggtcacag agcgcctcgg tctcggccag cacgtggctg gagaggagca
     6901 ccgtggcgcc gcggccgcgc tcgtcgcgca gaagccgctt cacctcggcg cgacccaccg
     6961 gatcgaggcc cgacatcggc tcgtccagga ccaggaggga gggccggttc aggagcgcct
     7021 gggccaggcc cagccgctgc gtcatgccct tggagaaggt gcggagcgct ttcgaggcgt
     7081 gctggcccag tcccagccgc tcgagcagct ccatcccgcg ggcgcgccgc tcctcgcgcg
     7141 gcatgccgaa gagcgaaccc accaggtcga gatactccgt cgcggtgaga tggtcgtaga
     7201 aacacggatt ctcgggcagg taacccagcc ggcggcgcgc ctcgggatcg cggggatcgc
     7261 gtccttcgat ccggaccacg cccgcggtcg ggcgcgagag gccgaggagg at
//
LOCUS       DASSLZ010000176        25851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_567998, whole genome shotgun sequence.
ACCESSION   DASSLZ010000176 DASSLZ010000000
VERSION     DASSLZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 25851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25851
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567998"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VFM00_06820"
     CDS             <1..962
                     /locus_tag="VFM00_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7497995.1"
                     /translation="PPMHSVSLALDSLDQPIVAFSDFASNTLNVARKTSAGWDLETVP
                     GTTFGKDTNLSLGAQDAPHIMYSDGSLRYVTRPGSEWVVETVADGASGACMKLDASGA
                     PHVAYVGRGGIQYSHAIQCNLVFGPNGISSQRHGTWITAYLESADFAIASVNPATIRL
                     EGTVACESKFATIGDHDADGIPDLMVKFRTEDALPLLAAGATSLAMTGRLATGEEFKV
                     DGAIRLTDTPTATAGLSLRILSPPGAAQVEYVLESPQGRSARLQVFDVRGRLVRSWDD
                     APSQAVHGWDGRGSGGSRVGSGVYFLLAEADGQRVSKRLVVSR"
     gene            1172..1537
                     /locus_tag="VFM00_06825"
     CDS             1172..1537
                     /locus_tag="VFM00_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7497996.1"
                     /translation="MALIVRKEDGITILTPAGMLLGGKETDEFEEKITELDKAGDRML
                     ALDMGQTTFMTSIAIAAVVRAHISYSKRGAQVKICALDKHIRHIFEITKLTCVFKENL
                     HATLEEGLASFRGTTSVAA"
     gene            complement(1538..3202)
                     /locus_tag="VFM00_06830"
     CDS             complement(1538..3202)
                     /locus_tag="VFM00_06830"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HET7497997.1"
                     /translation="MRPQAQGWAILGSRAAAAGPTAATMLTAAVMLVAAALALAPGTA
                     SARYLPQERVPVSSPVYRDLERLMASYDRAPLSFSTRPLRVQEILAYLNSLVVEYPSS
                     AGDPAMIRARRLVDPDAPGARAALITVRDDSTEVATFSPYVSVAYEDDPKNRPDINRD
                     YRVGAQVVAALDSTTIFVFDGYEGTASQGGRGTPNFGRLNSVIEGVDLNSWMDEAYAE
                     LKLARLRVLVGHASLRWGPGRDGTLALSDAAPPLDMIRLEAGFARKWRGQSFVALLDP
                     GPQTYLAGHRLEWNLRDMRLGVTELARFDGTSQALLYSIPIVPYSFWEKRPKSAPDGA
                     VPGDTTGLALSKNNVLLAVDLSWNVRRGYRLWGEFLVDDISFSSDYKPDMIGWQAGME
                     ERWLLGGGGGGAGEPQPLRAATLSLEYNRVNNYTYSAWHGHNFDFQGFPVGFVYGPDV
                     MQFAAEATYEHGANWELRVRGELRRKGEGFIGDAWTREQGKVDAGAISGIVERDARIA
                     TRLTWTPSRMLRVEGTVGYASFKNEDHRNVGVENETPFRLAARLEW"
     gene            3346..3921
                     /locus_tag="VFM00_06835"
     CDS             3346..3921
                     /locus_tag="VFM00_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7497998.1"
                     /translation="MPTLETYRSGDRAALLAIFDGNTPRFFDPHERELFAAFLDRPRG
                     DFRVLRDDERTIIGCGGVMVDEDGTASMVWGMIAPPHQGTGFGWWMALERLSWIAAMP
                     EARRVIMDTSQETAGFYVKLGFRTVSVRPDGYGPGLHRHDLEMPVDDAFRKRFGAGAS
                     GTGATGTGVTGGRLERPFPEATPPEPRSTHA"
     gene            complement(3887..5476)
                     /locus_tag="VFM00_06840"
     CDS             complement(3887..5476)
                     /locus_tag="VFM00_06840"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HET7497999.1"
                     /translation="MIEREIKFRLPEGVGADRVRQAVESAGFRLTPGLSLAHEDRYLD
                     TDDWALYRAGIALRLRAGARGVRLEAKTIRSTSDKALVRTEWSQEAPEGEPPWAMLAT
                     GHVAALLQPLHGLHVLERLRVRARLRNDRECFGWLRGDTPLGSLTVDHVNAMNGSDTA
                     PVAFDEVEIELLEEEPALERLRESPEERAARPNPDAALDEARRAVESALGVEANVASK
                     LATALAAAGERAPERDERAYAIHPADRLTDVAHKTFAKQFERMIWNEPGTRLGIDPEY
                     LHDMRVATRRLRTAMDLFETAIPDHPRRAFADDLRWVGRGLGRVRDRDVALQHVAELA
                     ADAPDLEIPAWRIFRHSLELERSRMRVRLIERLDSERYAALVERARAWIAAPAPAGEE
                     VPAGGSPAYTVAPRLIAERMGALAAAYDEAERLVDQPSLHAFRIAAKRARYAHEYFGD
                     TGGPRSSRRAKRLAGLQDFLGEHQDAVMLLRRLRKYAKSVPARDRELTLSVGSAMGHL
                     ERAARMRRGDLRRAWSEAREE"
     gene            complement(5582..6088)
                     /gene="sixA"
                     /locus_tag="VFM00_06845"
     CDS             complement(5582..6088)
                     /gene="sixA"
                     /locus_tag="VFM00_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HET7498000.1"
                     /translation="MDLYIVRHAIAVDRGDPAFPDDDERPLSPEGIHKFRLASRGLSE
                     LGLKLKRIASSPLLRARQTAEILRDAVAKNVEITIIEELRPETPFEQTANAIASQGDG
                     GIAVVGHEPHLSGLTSYLLVGGGDLAAIEFKKGAVARLAFPDRPAAGAGTLEWLIQPG
                     ALRLLAKD"
     gene            6169..6915
                     /locus_tag="VFM00_06850"
     CDS             6169..6915
                     /locus_tag="VFM00_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498001.1"
                     /translation="MRLWIGIGPAVAVSAALLAGCAKKHQPTAPTPGSPMPQTQLTGV
                     FVGGSENGLLDVTIDLGSLAMPGDTVVSALGVMSKDGGGVVNLTGTYDTATDSLRLSG
                     QGFELAGLYLEGGAPIRIEGRFVGPADSGSGLFTALPGRRPSAVHAFCATYENSAATM
                     WGTLNFAVAGNGVAGFEVVDGETAVVAVQGTAAGTTGTTRSLAFAGSDFIGNGSWNMS
                     TGHVAGVWASPRGSGVWSGDLCIAGTTGSE"
     gene            complement(6920..7879)
                     /locus_tag="VFM00_06855"
     CDS             complement(6920..7879)
                     /locus_tag="VFM00_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498002.1"
                     /translation="MSEQDEERQEAEALRLPPEAERARAALRAAPPLRADPAFRERLK
                     QEFVSGAIEVTGSRVRRLRANGESGPSRLAGAPRLKRGEFPGGHRRGVPTLAWVVASL
                     AAAAALVVVFGSLNRGPTWWVTAARGDGTVRVDGQTVALDDRDAMRRLLVPGAEIELT
                     GNAELDICSNGVLAVQLAPGTRMTLPPPPGRWLGRRTELYARSGELRVTTGKNFPGAQ
                     LAVMSPTAAVEIKGTTVAIIIEDTGTCVCVYEGSAMVGRLRGGEFADMHAVPGGMRRY
                     VYKDERAAVKDPDLHDMRAEERGKLASFRDSQRPWLMGEGTAD"
     gene            complement(7876..8409)
                     /locus_tag="VFM00_06860"
     CDS             complement(7876..8409)
                     /locus_tag="VFM00_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7498003.1"
                     /translation="MRRRDPRALGDFFEAYFDRVFSLLKRLLGDPVAAQDAAQEVFLK
                     IHRGAHQLDIARDPGPWVMAIATNVCRDLWRSGAYRMTATAASLEATPGLAETLASSA
                     RDPEADAVSAERARLVQEALRHLREPLREVVVLREYEGLGYDQIAAITVLNEAAVRKR
                     YSRALSELGKALEKAGL"
     gene            8662..10203
                     /locus_tag="VFM00_06865"
     CDS             8662..10203
                     /locus_tag="VFM00_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET7498004.1"
                     /translation="MDVGSNAMRLIIAEFKSPTRYKVVERVRMPVRLGASVFKTNRID
                     SETLEAAIEAFRQFRKTMEGHGVVVHRAVATSATREAKNRAAFLERVHTETGIDLELI
                     RGTEEARLVALGVRSKLSLDRGISMILDVGGGSSEIALVGHGEILMVESHDVGTVRLL
                     ERTGHAKHDKTLHLIHAILKSSRFPVLDSFKRRKLARLVGTGGNIETIATLNLSAVAS
                     RNGKNAKNGKDTKSEERPPARLTIARLRQTLHRLARVSPDERVKRYDLRPDRADVIVP
                     AGAIFEYVASRVGAKEIWVPFTGLVDGVLIDVARTAGAEGMKQLEVSQTRNAALALLK
                     KYEADPKHAEHVSELACDLFDQLKSLHKLSKRDRLLLDLAALLHEVGNFIGAPGHHRH
                     AYYIISESPILGLTDEELQIVANVARYHRKAMPDADHECYAELDEDAQDRVRALAAIL
                     RVADALDHDHKQKVLRVKAKAGDSKLRLKIRARGDVLLDEWALENKGDLFHEEFGLEP
                     VVENG"
     gene            10196..11836
                     /locus_tag="VFM00_06870"
     CDS             10196..11836
                     /locus_tag="VFM00_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET7498005.1"
                     /translation="MAERVPDLPEKPDAGAKPARSGPTPVPTAAAPERPPILSVIDVG
                     ASGIRMLIAELSPDGSVRTLEELERPIALGRDTFQTGSLSMASIRKAVNVLRQYRAVM
                     DTYGVQHTRAVATSAVRGALNRDTFVDRVFLATGIELEVIEGSQESLLTFAAVQRYME
                     AHPEYREGEALLLSLGGGATEWALVRNGQVAASITHDMGTLRMREVLRADAGDRRARA
                     RLLQHNAREMMNVVKRALPFERVTRLVAVGSEARFAARVIAGKESESLDLTAIAAKDL
                     KKLADQILPLTPEQIAQAYSVPPNETELLAPALFAYAELTRLNQVDQLLVARASMREG
                     LVLHMVRSIRSGSTVLFPDQTIAAAVNLARKYAADEKHGLQTAALARAIFQATFPQHQ
                     MGEREQLLLEVASIVHDIGEFVASRGHHRHTYYLLVHSEVFGLSQMDLEIVANIARYH
                     RRGVPQADHPSYASLPRPARLTVNRLSAILRVADALDKSHSQRVQDPKITVSADELRI
                     QVDSTEDLGLERLSLDAKSGLFEEVFGLKPVIVEGPTP"
     gene            12049..15495
                     /locus_tag="VFM00_06875"
     CDS             12049..15495
                     /locus_tag="VFM00_06875"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET7498006.1"
                     /translation="MLLAVVASASLISVRPAAAQYLWIDTNGDGVNTSADVVAPSGAT
                     TIDVWLATDHNANGDPAVCASGDAALTMAAYYVVLRATNGTMNWGSATNLIAAFTTQV
                     PSTSDATEREFGFYGGAGLAPGSYHVATLSSTVASGTPSIAFAPQGALDPGFRTGFGS
                     ACSGTDFDNLIKLGVDFTDAAGIAYGGAANHPPVLAAIADVSMGEGEIHDVPVSATDA
                     DGDVVTLALTSSPSWASLLSSPGAGTASGTVHLAPGFEDAGDASLTVKAADAVSNDQK
                     TFAVHVANTNRAPEIAPIAAMNLAEGVLLDTPIQASDADGDACTFFVASGPLFLTVST
                     VSSSFGTTFGNLHAAPGFADAGTYEVIIGARDTFGAADSTTYLILVREQDRPPVVTFP
                     GPISGTEGEPITFTISATDPDGDMVMYQTDGLPAGAQLTDQMNGTALFRWTPGFDQAG
                     SYDLMITADDMNGSAIHGDVSITVADVTDPVAIARPDDMTVIEGDVQEQPLLGFSASG
                     GALTFEKVSGPAFVSVTTTDPGTGTAHGVVHLAPGAADGGDATVTLAVTDGTNRDETS
                     FVIHVLDASTLPGQAPFQPPFIHLGVGLIPHTVIVADMNEDGIPDLVTANVGANTLSI
                     FPGQGSLAFGTRINLAAAVRPHTVVAKDLNHDGHLDLVVSHIGSNSFGVWLGGGNGTF
                     SARHDYQLSGSPVYLGVYDFNEDGNLDVVATDQTHGGIGIALGLGDGTFGAATFIPTG
                     QNTHGLVGADLNHDGHMDIAATNSTTPGTVSVLLGKGDGTFQPKIDFSTSSPHTVSAG
                     DLDQDGDIDLVVCDFDTGRITIALGHGDGTFATLPEIPTGTNPHASAVGDVDGDGKLD
                     IVVANQASNTLSMLLGRGGPSWAPKIDYPVGAGAHNVAIADLDGDHLPEVSCSNIVAN
                     TVTILKNRGHVTHPARVFTAGPRATLMLRASGPDYALHVESADGAFAATDIDLASVEL
                     RSPGTGSVDHIEADVAKGMTTADADQNGVVEAVIGFTRADLRNLFSGVVGRKSMTVTL
                     QGRLADASSFSGTATIDLVGAGAPGKPVANASVAPNPLNPVGVLTFTLTRSEAVTVRL
                     YDIRGRLVRRSMESRPLDAGIYRIAVDGTTDQGGPLASGVYFFRITTRDGDVSGRFAV
                     LK"
     gene            complement(15629..17590)
                     /locus_tag="VFM00_06880"
     CDS             complement(15629..17590)
                     /locus_tag="VFM00_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HET7498007.1"
                     /translation="MPSERVAIVGSPNSGKSSLFNRLTGLRQKVANYPGVTVEKKVGS
                     CRLPSGKHVDIVDLPGTYSLAPRSPDEAIVRDVLDGHCAPEERADLVVAVVDATNLER
                     QLYLALQILATGRPAVLVLNLIDAAQAQGIAIDVPAMERMLGVPVLAVSAKTGYGVDR
                     LRAALDARAAVNGNGNGNGNGNGHVEGHANGYVNGHGNGNGHRLTLEQALDPALGYAS
                     VEAIIKAAVRRVPGRQAPRAGLDRILTHKVLGPIILVLFMGAVFQSIYAWASPFMDLI
                     ASGFAALAGAAQATLPAGPIRSLLVDGVLAGAGTVASFIPQLAILFFFIALMEDTGYM
                     ARAAFIMDRVMGAVGLPGRAFLPLLSSFACAIPGIMATRTIENRNDRLTTIMIAPLMT
                     CSARLPVYALLIGAFIPDRFVLGILNLRGLTLLSLYLFGILSALAVAAVLKRTVLRGP
                     KPLYVMELPHYRMPSLRSIAMTIRNRCLLFLQKAGTVIVAVSLVLWFLASYPVSKPGT
                     PPSEALRHSFAGQLGRAIEPVIRPLGFDWKMGVGLISSVAAREVMISTMATVYSLDGS
                     GDPSMSLKQTLPKVRNDRTGALAYTPLVAVSLMVFFALACQCMSTVAVARRETNSWRW
                     PLFMLLFMNAIAWAASFMVFQGGRLLGWG"
     gene            complement(17580..17813)
                     /locus_tag="VFM00_06885"
     CDS             complement(17580..17813)
                     /locus_tag="VFM00_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HET7498008.1"
                     /translation="MTSRTLALLAPGDRAVVRGILAEKEFAMRLMELGLTPGTEVALI
                     RRAPFGDPLELAVRGSHFSIRRSEAERIHVDAL"
     gene            complement(17810..18373)
                     /locus_tag="VFM00_06890"
     CDS             complement(17810..18373)
                     /locus_tag="VFM00_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET7498009.1"
                     /translation="METWKRFHEKQLTHSMAHYLQAIASLTRGGGTSVSAIAERLGVS
                     KAGVTSMLRSLTTRGLAKHEPYGDVTLTVEGQRLAARTERSRDVLTEFFGEILGLDPA
                     TAEEDACMIEHLVSPDSMVRFLRLTAFLRSDHPAAERFRDEFREYQNRCESGTESAHC
                     PVCHGRCLRVALEALIDPVDGGKESLS"
     gene            18493..20625
                     /gene="ppk1"
                     /locus_tag="VFM00_06895"
     CDS             18493..20625
                     /gene="ppk1"
                     /locus_tag="VFM00_06895"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HET7498010.1"
                     /translation="MTEPKDTQGRYLDRAMTWLAFNGRVLEEAQDPQSAILERARFLG
                     IFASNLDEFFMIRVASIRDRVRTGADQRSAAGQTQAERLTSVQARARELTAAAYTLWE
                     QGIVPGLRDAGIRFLRESELEPNERAFVDRLFMRELLPVLTPAAVAEDTPFPRLFHLA
                     LHLAVLLAKPGEEERLAVVQLPRTGTRWVQVSGGARADGQTPAQRFILIEEAARAHLG
                     ELFAGYEVREAVAFRVTRDADFATDDQEEENLVDAVRQVLRQRMRGDPVRLEIAREAS
                     DAVVERLRIALELEEREVYRVSGPLDFRFLMDFARQPSLKAPRAPAWPPQPVPGVPPL
                     DGIWDAIQERDILLFHPYETFEPVLRLLVLAAEDPSVLAIKQVLYRTSSTSEVVNALE
                     RAAESGKQVTVLVELQARFDEERNVNWARRLEDAGAQVLYGLAGLKTHAKALLIVRRG
                     PGGIERYAHVGTGNYNERTAAEYSDVGLLSADEDLCADLTGFFNVVTGYSEPLPWRRI
                     EMAPLGLRSRLLALIHREIAKATTEEPGLIRAKMNALLDIPLIEALYEASNAGVRIDL
                     NVRGSCLLRPGVKGMSENVRVVSLVDRFLEHARIFEFRNGGDVETFIASADWMPRNLD
                     RRVELMTPIVDPEHRARLGRILDAMFADNVKARELRSDGSYVKRDGRKKATRAQELFW
                     EEARAAASRTEDPDRSVFQPLRRAPNAG"
     gene            20695..21249
                     /locus_tag="VFM00_06900"
     CDS             20695..21249
                     /locus_tag="VFM00_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498011.1"
                     /translation="MASWVNRVIGAAQLRSATYEEVEADKSANLQALGVVVLSAIAAG
                     FSMAQGGGRAIGVGILAALVAWVLWSALTWLIGTKFLSTPETHADIGELLRTTGFAAA
                     PGMLRIFGGIPVVGFVFNLVAGLWMLAAFVVAVRQALDYRSTGRALLVCGIGWLVWFA
                     VIAWTFLLLGASVAGFQALRGNIQ"
     gene            complement(21257..22837)
                     /locus_tag="VFM00_06905"
     CDS             complement(21257..22837)
                     /locus_tag="VFM00_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498012.1"
                     /translation="MSERTKRDTRAGLLAVAALAAALGGIAPGLRAEPAEFLPVRHFA
                     YEEIEALAARGRLDSIAVYSRPLARIDVARALLRAERLDSTIAADLHFQRLERELARE
                     LTDLGAPPAEPETGPLVDAGPREERFRLAAAGHLRGDYQERRDPHYRFRDESSASARM
                     SLQLWPAFAAFEELGVTRVRSARDWIDAVIAHTDLETAVLRAEITARAGALTVAAGYD
                     DFRWGPGRRGTLLLSDAAGPMGFLYHSVRLGGRVTASAVTGQLSRVDRRYLAAHRIEA
                     NVSRALTVGLGEAVRYRSDNVDLVYALGILPYAIVERIHIRDVSSDSLRGLERANVMA
                     SADLAWRPTRDVSLTGELLVDDFTTEDRTMPDRFAWQLGLRSDRPFGARAIRLLGEYT
                     RVRNFTYSVDYGQNFIYRDRPLGFALGPDVENVFVEAVYDWSRDWQFRWSGDFTNHGE
                     GRLGDAFLEGTGPVSNASLTGIVERRREVFGDARWMPRDNVDLSLGAGYGRVRNENHV
                     AGRERNEWLARLAFDVRY"
     gene            23082..23927
                     /locus_tag="VFM00_06910"
     CDS             23082..23927
                     /locus_tag="VFM00_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7498013.1"
                     /translation="MQRTTIITAAIFAGALATAPWSASAGPLDYQGLAFQSQGSGANS
                     VLAGVETGNGDAVPVFASTSSSSFSERPIYRRRPYRDRYRDRYDDNDQPRYFGAFGAG
                     TFDPDDQPGNGLWLNGELGSEVGDALDLGVRVNWYHRQSDNSQVVSTYTDEAGNVHER
                     VLDTNNIETNLVPLMAILRVRFPVSRDFQPYVGGGIGWEWLTVSGTDSTGFDFQDDYD
                     GFGGQVFAGLNVGVSPTLNLYGEALWNKSTVSARFFDPFVGATIKDEIDMDGLGLHGG
                     LRFSF"
     gene            24088..24678
                     /locus_tag="VFM00_06915"
     CDS             24088..24678
                     /locus_tag="VFM00_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7498014.1"
                     /translation="MRVAALGVALVAITGLAATAPRAWAQTSSTSARTEDVSLGFKGM
                     GARIGLVDPEGASSTVDLGVHVDAGEFARNIHLQPIVEYWSVGQDVTILSSTYSVNSK
                     DFSVGADINIDFPLQDSRVTPYAGGGLGLHWLKETNDAPGGAEFSDTKLGLNLQGGVR
                     TDAMPNLALFGELRYNFVSDANQLKILGGFTYRFIY"
     gene            24704..25513
                     /locus_tag="VFM00_06920"
     CDS             24704..25513
                     /locus_tag="VFM00_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498015.1"
                     /translation="MRRLGIVAFSLAVVVGVSACQKKQTETSTSASDTTMSAESPYPV
                     EETPPAATTTPPAENPPATSGTRTTTHTTTPKTTHSSGTTTASKVTVHIPVGTTMDLV
                     MVTDASTKTANIGDPIEAKLAAPLVVDGKVVAEEGSVVRGKISDLKRASHAKDQEGRA
                     FLKYDFTSIETVGGTKSLNATVTSSEGKELEAKSTSTRDKLLIGGGTVAGAVIGKVAG
                     GSTKSTIIGAIGGAAVGAGAVAASKGHELEIPAGSKVVLKTEEPITVMMAK"
BASE COUNT         4061 a         8882 c         9031 g         3877 t
ORIGIN      
        1 cgccaccgat gcactccgtg tcgctcgcgc tggactcgct ggaccagccg atcgtcgcgt
       61 tctccgactt cgcatccaac acgctgaatg tcgcgcgaaa gacgtccgcg ggctgggacc
      121 tcgagacggt gcccggcacg acgttcggca aggacacgaa cctctccctc ggtgcgcagg
      181 acgcgccgca catcatgtac agcgacggct ctcttcgcta cgtcacgcgg ccgggttccg
      241 agtgggtggt cgagacggtg gccgacggcg caagcggcgc gtgcatgaag ctggacgcct
      301 cgggcgcgcc gcacgtggct tatgtggggc gcggcggcat ccagtactcg cacgcgatcc
      361 agtgcaacct ggtcttcggc ccgaatggca tcagctccca gcgccatggg acctggatca
      421 ccgcctatct cgagtcggcg gacttcgcga tcgcgtcggt gaacccggcc acgatacggc
      481 tggagggcac cgtcgcctgt gaatcgaagt tcgcgacgat cggcgaccac gatgccgacg
      541 gaatcccgga tctcatggtc aagttccgga cggaggatgc gctgcctctg ctggcggccg
      601 gagccacgtc gctggccatg accggacggc tcgcgacggg cgaggagttc aaggtggacg
      661 gggcgatccg gctgaccgac acgccgaccg cgaccgccgg cctatcgctc cggatcctct
      721 cgccgccggg cgcggcacag gtcgaatacg tgctggaaag cccccaggga cggagcgcgc
      781 gacttcaggt gttcgacgta cgcggacgat tggtgcggag ctgggacgac gctccatccc
      841 aggcggtcca tgggtgggac ggcaggggat ccggaggaag ccgcgtgggc tccggcgtct
      901 acttcctgct cgccgaagcg gacgggcagc gggtttcgaa gcggctcgtc gtgagccggt
      961 agtcgcggtc gccgagacct cgccggcgcg aggcaggaca aattaatatc tatattaatt
     1021 ttaacacatc ctgggacgtg ttccttgtgc cggattggcc caccggctga ctggctccct
     1081 ccgaaacaat tccggttcct tttccgtcat taagacgttc tctgtgtata gaaaatgggg
     1141 agcggtgctc cctgtgggga aggagctggt catggcactc atcgttcgaa aagaggacgg
     1201 aatcacgatc ctgaccccgg ccgggatgct tctggggggc aaggagaccg acgagttcga
     1261 ggaaaagatc accgagctgg acaaggccgg ggaccggatg ctggcgctgg acatgggcca
     1321 gacgaccttc atgaccagca tcgcgatcgc cgcggtcgtc cgcgcgcaca tcagctattc
     1381 caagcgcggg gcccaggtca agatctgcgc gctggacaag cacatccggc acatcttcga
     1441 gatcaccaag ctcacctgcg tgttcaagga aaacctccac gccacgctgg aggaggggtt
     1501 ggcgagcttc cggggaacga cgtcggtggc ggcttagtca ccactccagg cgcgccgcca
     1561 accggaacgg ggtttcgttc tccacgccca cattccggtg atcctcgttc ttgaagctcg
     1621 cgtagcccac ggtaccttcc acgcggagca tgcggctcgg cgtccacgtg agccgggtcg
     1681 cgatccgcgc gtcgcgctcc acgatccctg agatggcgcc ggcgtccacc ttgccctgtt
     1741 cgcgcgtcca ggcgtcgccg atgaagccct cgcccttccg ccgcagctcg ccgcgcacgc
     1801 gcaattccca gttcgcgccg tgctcgtacg tggcctcggc cgcgaactgc atgacgtcgg
     1861 ggccgtagac gaacccgacc gggaagccct ggaaatcgaa gttgtgaccg tgccacgcgg
     1921 agtaggtgta gttgttcacc cgattgtatt cgagcgataa cgtggcggcc cgcaggggct
     1981 ggggttctcc cgcgccgcct ccaccgcccc cgagcagcca ccgctcctcc atgcccgcct
     2041 gccagccgat catgtccggc ttgtagtcac tcgagaacga gatgtcgtcc accaggaact
     2101 cgccccagag ccggtagccg cgccgcacgt tccaggacag gtccacggcc aggagcacgt
     2161 tgttcttgga gagcgcgagg ccggtcgtgt cgcccgggac cgcgccgtcg ggcgcggact
     2221 tgggccgctt ctcccagaag gagtagggca cgatcgggat gctgtagagg agcgcctggc
     2281 tcgtgccgtc gaaccgcgcc agctcggtga ctccaagccg catgtcgcgg aggttccact
     2341 ccagccggtg cccggccagg taggtctgcg ggccggggtc caggagtgcg acgaacgact
     2401 gaccgcgcca cttccgcgca aaccccgcct ccaggcggat catgtccagc gggggcgccg
     2461 cgtcggagag cgcgagcgtt ccgtcccgtc ccgggcccca ccgcagcgag gcgtgcccga
     2521 cgaggacgcg caagcgcgcg agcttcagct cggcgtaggc ctcgtccatc caggagttca
     2581 ggtccacccc ctcgatgacc gagttcaggc ggccgaagtt gggcgtgccg cggcccccct
     2641 gcgaggcggt gccctcgtag ccgtcgaaga cgaagatcgt cgtggaatcg agcgcggcca
     2701 cgacctgggc gccgacgcgg tagtcccggt tgatgtcggg acggttcttg ggatcgtcct
     2761 cgtacgccac cgagacgtag ggggagaagg tggccacttc cgtcgagtcg tcgcggacgg
     2821 tgatcagcgc cgcgcgcgcg cccggggcgt ccgggtcgac caagcgccgc gcgcggatca
     2881 tcgcgggatc gccggccgaa gaggggtact ccacgaccag cgagttcagg tacgccagga
     2941 tctcctgcac gcggagaggg cgtgtcgaga aggagagtgg ggcgcggtcg tagctcgcca
     3001 tgagccgctc gagatcgcga tagacggggc tcgagaccgg cacccgttcc tggggaaggt
     3061 accgggcgga cgccgttccg ggggcgaggg ccagcgccgc agcgaccagc atcaccgccg
     3121 cggtcagcat ggtggcggcg gtcggtcctg ccgccgcggc cctcgaaccg aggattgccc
     3181 atccctgggc ctggggtctc atggcgggac agggtagccg gggctcctcc gggtgtccac
     3241 gtgggagcgc ctacccgatc cggcgagggc ggcgggaagg gggttgcggg cgctccggag
     3301 gggtgtagac tcccgattat ccagccgaca caagggaagc cgaccatgcc aacgctcgaa
     3361 acgtatcgtt ccggcgaccg cgccgcattg ctcgcgatct tcgacgggaa cacgccccga
     3421 ttcttcgatc cgcacgaacg ggaattgttc gcggcgttcc tggaccgccc tcgcggcgat
     3481 ttccgcgtgc tgcgcgatga cgagcggacg atcatcggct gcggcggcgt catggtcgac
     3541 gaggacggaa cggccagcat ggtctggggg atgatcgcgc ccccgcacca ggggaccggg
     3601 ttcggctggt ggatggcgct ggagcggctc tcctggatcg cggcgatgcc ggaggcgcgg
     3661 cgcgtgatca tggacacgag ccaggagacg gccgggttct acgtcaagct ggggttccgg
     3721 acggtgtcgg tgcgccccga cggctacgga ccgggtctcc accggcacga tctcgagatg
     3781 ccggtggacg atgcgttccg gaagcggttc ggcgcgggcg ccagcggcac gggcgccacc
     3841 ggcacgggcg tcaccggcgg ccgcctcgag cggcccttcc ccgaagctac tcctcccgag
     3901 cctcgctcca cgcacgcctg agatcgccgc ggcgcatccg cgccgcgcgc tccaggtggc
     3961 ccatcgcact tcccaccgag agcgtcagct cccgatcgcg cgccggcacc gacttcgcgt
     4021 acttccgcag gcggcgcagg agcatgaccg cgtcctggtg ctctccgaga aaatcttgga
     4081 gtccggcgag ccgcttggcg cgccgcgagg accgggggcc gccggtgtcg ccgaaatact
     4141 cgtgcgcgta gcgcgcgcgc ttggcggcga tgcggaacgc gtgcagcgag ggctggtcca
     4201 ccagccgctc ggcctcatcg tacgccgcgg ccagcgcgcc catgcgctcg gcgatcagcc
     4261 gcggcgcgac ggtgtaggcg ggggagccgc cggccgggac ctcttcgccc gcgggcgccg
     4321 gcgccgcgat ccaggcgcgc gcccgctcga ccaaggccgc gtagcgctcg gagtccagcc
     4381 gctcgatcag gcgcacgcgc atccgggaac gctccagctc gagcgagtgg cggaagatgc
     4441 gccaagccgg gatctcgagg tccggggcgt cggcggccag ctccgccacg tgctggagcg
     4501 ccacgtcgcg gtcgcgcacg cgccccagcc cgcgtccgac ccagcggaga tcgtcggcga
     4561 acgcgcgccg cggatgatcg gggattgccg tctcgaagag gtccatcgcc gtgcgcaggc
     4621 ggcgggtcgc cacgcgcatg tcgtggagat attcgggatc gatccccagg cgcgtcccgg
     4681 gctcgttcca gatcatgcgc tcgaactgtt tggcgaacgt cttgtgcgcg acgtcggtga
     4741 ggcggtcggc gggatggatc gcgtaggcgc gctcgtcgcg ctcgggcgcc cgctcgcccg
     4801 cggcggcgag ggcggtggcg agcttggacg cgacgttcgc ctcgacgccg agcgcggact
     4861 cgacggcgcg acgcgcctcg tcgagggcgg cgtccggatt cggccgggcg gcgcgctctt
     4921 ccgggctctc gcggagccgc tcgagcgcgg gctcctcttc gaggagctcg atctccacct
     4981 cgtcgaacgc gaccggcgcc gtgtcggacc cgttcatcgc gttcacgtgg tccacggtca
     5041 aggagccgag cggcgtgtcg ccgcgcagcc acccgaagca ctcccggtcg ttgcgcagcc
     5101 gggcccgcac gcggaggcgc tccagaacgt ggagcccatg gagcggctga agaagcgccg
     5161 cgacgtgccc ggtcgcgagc atcgcccagg gcggctcgcc ctcgggcgct tcctgactcc
     5221 attcggtgcg gacgagcgcc ttatcgctgg tggatcggat cgtcttggct tccagccgca
     5281 ccccgcgcgc gcccgcgcgc aagcgcagcg cgatccccgc gcgatagagc gcccagtcgt
     5341 cggtatcgag gtagcggtct tcgtgggcga gggaaaggcc cggcgtcagg cggaatccgg
     5401 ccgactccac ggcttgccgc acgcggtcgg cgccgactcc ttccgggagc cggaatttga
     5461 tttcgcgctc gatcatgggc acggtgtggt ccttgcgcgg tcgcgtccgt gcggcgcgtc
     5521 gttgaggcgc tctcgaggcg ggccggcgct cgatgcgccg gcgggcttca gtcccggggc
     5581 gtcagtcctt ggcgaggagg cgaagcgcgc ccggctggat cagccactcg agcgttcccg
     5641 ccccggccgc cggccggtcg gggaacgcga ggcgcgccac ggctcccttc ttgaattcga
     5701 tcgctgccag atcgccgcca ccgacgagca ggtaggacgt gagtccgctc aggtgcggct
     5761 catggcccac caccgcgatg ccgccgtcgc cctgcgacgc gatggcgttg gcggtctgct
     5821 cgaagggcgt ctccggccgc agctcttcga tgatggtgat ctccacgttc ttggcgacgg
     5881 catcgcgcag gatttcggcc gtctgccgcg cgcgaaggag cgggctggac gcgatccgct
     5941 tcagcttgag ccccagctcc gagagccccc gcgacgccag gcggaacttg tggatcccct
     6001 ccggcgagag gggccgctcg tcgtcgtcgg gaaaggccgg gtctccccgg tcgaccgcga
     6061 tggcatggcg cacgatgtag agatccataa gtgtaagtgt gacaggggtt gaggggcggg
     6121 tcaacgtctc tcggatcgcc tgttctggcc gggtgttaga gtggttacat gagactctgg
     6181 atcggtatcg gcccggcggt cgccgtgtcc gccgctctgc tcgcgggatg cgcgaagaag
     6241 catcagccca cggcacccac gcctggctcg cccatgcccc agacccagct gaccggggta
     6301 ttcgtgggag gctccgagaa cgggctcctc gacgtgacca tcgatttggg atctctcgcc
     6361 atgcccggag acactgtcgt gtccgcactg ggcgtgatga gcaaggatgg cgggggcgtc
     6421 gtgaatctga ccgggaccta tgacaccgcg accgattcgc tgcggctcag cggccaaggc
     6481 tttgagctgg ccggcctcta cctcgagggc ggagcgccca tccggatcga ggggcgattc
     6541 gtcggacccg cggacagcgg cagcggcctg ttcactgcgc tgccaggtcg caggccctcc
     6601 gcggtccacg ccttctgcgc cacctacgag aactcagccg cgaccatgtg gggcacgctg
     6661 aatttcgcgg tggccgggaa cggcgtggcc ggtttcgaag tcgtcgacgg agaaaccgcc
     6721 gtcgtcgccg tgcagggtac cgccgcggga accaccggga cgacccggtc gctcgccttc
     6781 gccggcagcg atttcatcgg caacggcagc tggaacatgt cgacgggcca tgtcgcgggc
     6841 gtatgggcca gcccgcgcgg ctccggcgtt tggagcggcg atctctgcat cgccggaacg
     6901 acgggctcgg agtaacgacc taatccgccg tgccttctcc catcagccag gggcgctggg
     6961 aatcgcggaa cgacgcgagc ttgcctcgct cctcggcgcg catgtcgtgc agatcggggt
     7021 ccttgaccgc ggcgcgctcg tccttgtaga cgtagcggcg catgccgccc gggacggcgt
     7081 gcatgtcggc gaactcgccg ccgcggaggc gtcccaccat ggcggagcct tcgtagacgc
     7141 agacgcaggt gccggtgtcc tcgatgatga tcgcgaccgt cgtgcccttg atctcgacgg
     7201 cggcggtcgg gctcatcacc gcgagctggg cgccggggaa attctttccc gtggtcacgc
     7261 gcagctcgcc gcttcgcgcg tacagctcgg tccggcgtcc cagccagcga cccggcggcg
     7321 gcggaagggt catccgcgtg ccgggggcga gctgcaccgc cagcacgccg ttgctgcaga
     7381 tatcgagctc ggcgtttccg gtcagctcga tctccgcccc ggggacgagc aggcgtcgca
     7441 tcgcgtcgcg gtcgtcgagg gccacggtct ggccgtcgac gcgcaccgtg ccgtcgccgc
     7501 gcgcggcggt gacccaccac gtggggccgc ggttcaggga gccgaagacg accacgagcg
     7561 ccgccgccgc cgcgagcgag gccacgaccc acgccagcgt cggcacgccg cggcgatggc
     7621 cgcccgggaa ttcgccgcgc ttcaggcgcg gggcgcccgc cagacgcgac ggtcccgact
     7681 ctccgttggc gcggaggcgc cgcacgcgcg atccggtcac ctcgatcgcg cccgagacga
     7741 actcctgctt caggcgctcg cgaaacgcgg gatcggcgcg gagcggcggc gccgcgcgaa
     7801 gggcggcgcg ggcgcgctcg gcctcggggg gcaaccgaag cgcctcggct tcctgccgct
     7861 cttcgtcctg ttcgctcata gtcccgcctt ctcgagggct ttgcccagct ccgacagcgc
     7921 gcgcgagtag cgcttgcgca ccgcggcttc gttcaaaacg gtgatcgcgg cgatctggtc
     7981 atagcccagc ccctcgtatt cgcgcagcac cacgacctcg cgcaacggct cgcgcagatg
     8041 ccgcagcgcc tcctggacca ggcgcgcgcg ctcggccgac accgcgtccg cctcggggtc
     8101 ccgcgcgctc gacgccagcg tctcggccag acccggcgtc gcctccagcg aggccgcggt
     8161 cgccgtcatc cggtacgcgc ccgagcgcca gagatcccgg cacacgttgg tggcgatcgc
     8221 catgacccag ggtccggggt cgcgcgcgat gtccagctgg tgcgcgccgc gatggatctt
     8281 caagaacact tcctgtgcgg cgtcctgtgc cgcgacgggg tcgccgagga ggcgcttcag
     8341 gagggagaac acgcgatcga agtaggcctc gaagaagtct ccgagagccc gtggatcgcg
     8401 ccgcctcacg ccctccaggt ccaacccgga cgtccggtcg ctcacaaccc gaccggccgc
     8461 cgcggcactc gcgtccgatt cgtatacgga cgaagcgctc ttttgtcccg gggtccggga
     8521 ggaggcatcc acgcggggaa gcctaccaca gggggtggcc ggcccgaacc gccgcgaggc
     8581 ttgacccctg gggcccgggc ccccaccatg ggggtaatgc ctacggcctc aagggcttcc
     8641 ttcccgatcc gctgcgccgg catggacgtc ggctccaacg ccatgcggct catcatcgcc
     8701 gagttcaaga gccccacccg ttataaggtg gtggagcggg tccggatgcc ggtccgcctg
     8761 ggcgccagcg tcttcaagac gaaccggatc gactccgaga ccctggaagc ggcgatcgag
     8821 gccttccgcc agttccggaa gacgatggag gggcatggcg tggtcgtgca ccgggcggtc
     8881 gcgacaagcg cgacccgaga ggccaagaac cgggccgcgt tcctggagcg ggtgcacacc
     8941 gagaccggga tcgacctgga gctgatccgc ggcaccgaag aggcgcgcct ggtggcgctg
     9001 ggggtgcgga gcaagctctc gctggatcgt gggatctcca tgatcctgga cgtcgggggc
     9061 ggctccagcg agatcgcgct ggtcggccac ggagagatcc tcatggtcga atcgcacgac
     9121 gtggggacgg tgcggctcct ggagcgcacc ggccacgcca agcacgacaa gacgctccac
     9181 ctcatccacg ccattctcaa gtcctcgcgc tttccggtcc tggattcctt caagcggcgg
     9241 aagctggcgc ggctggtcgg gacgggcggg aacatcgaga cgatcgcaac gctcaacctg
     9301 tccgccgtgg cttcccgaaa cggaaagaac gccaagaacg gcaaggacac gaagagcgag
     9361 gagcgcccgc ccgcgcggct cacgatcgcg cggctgcgcc agacgctgca ccggctggcc
     9421 cgggtgtccc ccgacgagcg cgtgaagcgc tacgacctcc gtcccgaccg cgccgacgtc
     9481 atcgttcccg cgggagcgat cttcgagtac gtcgcctcgc gcgtgggcgc caaggagatc
     9541 tgggtgccct tcaccgggct cgtggacggc gtgctgatcg acgtggcgcg caccgcgggc
     9601 gccgaaggga tgaagcagct ggaggtgagc cagacgcgga acgcggcgct cgcgctcctc
     9661 aagaaatacg aggccgatcc caagcacgcc gagcacgtga gcgagctggc ctgcgacctc
     9721 ttcgatcagc tcaagtcgct ccacaaactc tcgaaacgcg accgcctgct cctggacctg
     9781 gccgcgctgc tccacgaggt ggggaacttc atcggggcgc cggggcacca tcgccacgcc
     9841 tactacatca tctccgagtc cccgatcctg ggcctgaccg acgaggagct gcagatcgtg
     9901 gcgaacgtgg cccggtacca ccggaaggcg atgcccgacg ccgaccacga gtgctacgcg
     9961 gagctggacg aggacgcgca ggaccgcgtg cgcgcgctgg ccgccatcct gcgcgtggcc
    10021 gacgcgctgg accacgacca caagcagaag gtgctgcggg tcaaggccaa ggcgggcgac
    10081 tccaagctgc gactgaagat tcgtgcccgg ggcgacgtgt tgctggacga gtgggcgctg
    10141 gagaacaaag gcgacctctt ccacgaagag ttcgggctgg agccggtggt ggaaaatggc
    10201 tgagcgcgtt cccgatctcc ccgagaagcc cgacgccggc gcgaagccgg cccgctcggg
    10261 cccgacgccg gtccccaccg ctgcggctcc cgagcgcccg ccgatcctct ccgtcatcga
    10321 cgtcggcgcg agcgggatcc gcatgctgat cgcggagctt tcccccgacg gctcggtgcg
    10381 cacgctggaa gagctggagc ggccgatcgc gctgggacgc gacaccttcc agaccggcag
    10441 cctctccatg gcgagcatcc ggaaggcggt gaacgtcctc cgccagtacc gcgcggtcat
    10501 ggacacctac ggcgtgcagc acacgcgggc ggtggcgacc agcgccgtgc gcggggcgtt
    10561 gaaccgcgac accttcgtgg accgcgtgtt cctggccacc gggatcgagc tggaggtgat
    10621 cgagggttcg caggagagcc tgctcacctt cgcggccgtg cagcgctaca tggaagcgca
    10681 ccccgaatac cgcgaagggg aggcgctgct tctctccctg gggggcggtg ccacggaatg
    10741 ggctctggtg cggaatggtc aggtggcggc ctcgatcacg cacgacatgg ggacgctacg
    10801 catgcgcgag gtgctgcgcg cggacgccgg cgaccgacgg gcgcgggcgc gcctgttgca
    10861 gcacaacgcg cgcgagatga tgaacgtggt caagcgggcg cttccgttcg agcgcgtcac
    10921 gcgcctggtc gcggtgggat cggaggcgcg cttcgccgcg cgggtgatcg cggggaagga
    10981 gtcggagtcg ctggatctga ccgcgatcgc cgcgaaggac ttgaagaagc tcgccgacca
    11041 gatcctcccg ctcacccccg agcagatcgc gcaggcctac tcggtgccgc ccaacgagac
    11101 ggagcttctg gcgccggcgc tcttcgccta tgcggagctg acccggctga accaggtgga
    11161 ccagctcctg gtggcacgcg ccagcatgcg cgagggattg gtcctccaca tggtgcggtc
    11221 gatccggagc gggagcacgg tgctcttccc ggaccagacg atcgccgccg cggtgaacct
    11281 ggcccgcaag tacgcggccg acgagaagca cgggctgcag accgcggccc tcgcgcgcgc
    11341 gatcttccag gccacgtttc cgcagcatca gatgggggag cgggagcagc tccttctgga
    11401 ggtggcgtcg atcgtccacg acatcggcga gttcgtcgcc tcgcggggac atcaccggca
    11461 cacctactac ctgctggtgc actccgaggt gttcgggctc tcgcagatgg acctggaaat
    11521 cgtcgccaac atcgcgcgct accaccgccg cggggtgccg caggccgacc acccgtcgta
    11581 cgcctcgctc ccgcgcccgg cccgcctcac cgtgaaccgc ctctccgcca tccttcgcgt
    11641 ggccgatgcc ctggacaaga gccacagcca gcgcgtgcag gatccgaaga tcaccgtttc
    11701 ggccgacgag ctccgcatcc aggtcgacag caccgaggat ctcggtctgg aacggttgtc
    11761 cctcgacgcg aagtcggggc tgttcgaaga ggtgttcggg ctgaagcccg tgatcgtgga
    11821 aggtccgacc ccgtaacgcg ctgccgcgcc gctcccgacg gcgcctgccc cgcgataccc
    11881 tgcccgcgcc gccgccccgc gcttgtaact ctcgttttcc cttgcaattc cacgataaac
    11941 tgcgtatcat ttcgaacgac ctcatcgatc cctgcatcct ggaggtgtga cgtgcctacc
    12001 ccgcccgcgt cccctgactc tgcgtttcgc ctttcgcgaa tcgcgctgtt gcttctcgct
    12061 gtcgtcgcct cggcatcgct gatctcggta cgtcccgcgg ctgcccagta tctctggatc
    12121 gacacgaacg gtgacggcgt gaacacgagc gccgacgtcg tggcgcccag cggcgccacc
    12181 accatcgacg tctggctcgc caccgaccac aacgccaacg gcgatcccgc cgtgtgcgcg
    12241 agcggcgacg ccgccctcac gatggcggcc tattacgtcg tgctccgggc caccaacgga
    12301 accatgaact ggggatcggc cacgaacctg atcgccgcgt tcacgacgca ggtgccgtcc
    12361 acgtccgacg cgaccgagcg cgagttcggc ttctacggcg gcgcggggct ggcgccgggg
    12421 tcctatcacg tggccacgct ctcctccacc gtggcttcgg gaacgccgag catcgccttc
    12481 gcgccccagg gagccctgga tcccggcttc cggaccggat tcggctccgc ctgctccggg
    12541 accgacttcg acaacctgat caagctgggc gtcgacttca ccgacgcggc tggcatcgcc
    12601 tacggcggcg ccgcgaatca tccgcccgtt ctggcggcga tcgccgacgt gagcatgggc
    12661 gagggggaga ttcacgacgt gccggtctcg gccacggacg ccgacggcga cgtggtcacg
    12721 ctcgcgctca cgtcgtcgcc ctcctgggcc tcgctcctga gcagtcccgg cgcgggtacg
    12781 gcctcgggca ccgtccatct cgcgcccggc ttcgaggacg cgggggacgc ctcgctcacg
    12841 gtgaaggcgg ccgacgccgt atcgaacgac cagaagacct ttgcggtgca cgtcgcgaac
    12901 acgaaccgcg cgccggagat cgccccgatc gccgcgatga acctggccga aggggtgttg
    12961 ctggatacgc cgatccaggc gagcgacgcc gacggcgacg cctgcacttt cttcgtcgcc
    13021 tcggggcccc tgttcctcac ggtgagcacg gtctcctcct cgttcgggac cacgtttggc
    13081 aacctccacg ccgcgcccgg cttcgccgac gcgggaacct atgaagtgat catcggcgcg
    13141 cgggacacct tcggcgcggc cgactcgacg acgtacctga tcctggtgcg cgagcaggac
    13201 cggccgcccg tggtcacctt cccgggcccg atctccggca cggaagggga gccgatcacg
    13261 ttcacgatct cggccaccga tccggacggc gacatggtga tgtaccagac cgacgggctg
    13321 cccgcggggg cgcagctcac cgaccagatg aacggcacgg cgctcttccg gtggacgccc
    13381 ggcttcgatc aggcgggctc gtacgatctc atgatcacgg ccgacgacat gaacggctcc
    13441 gcgatccacg gcgacgtctc gatcacggtg gccgacgtga ccgatccggt ggcgatcgcg
    13501 cggccggacg acatgacggt gatcgaggga gacgtgcagg aacagccgct gctcggcttc
    13561 agcgcgtcgg gcggcgccct caccttcgag aaggtctccg gacccgcctt cgtctccgtc
    13621 accaccacgg atccgggaac gggcaccgcg cacggcgtgg tccacctggc accgggcgca
    13681 gccgatggcg gggacgccac cgtgacgctc gccgtgaccg acgggacgaa ccgcgacgag
    13741 acctcgttcg tcatccatgt cctggatgcc tcgacgcttc ccgggcaggc gcccttccag
    13801 ccgcccttca tccatctcgg cgtcggcctc atcccgcaca cggtgatcgt cgcggacatg
    13861 aatgaggacg ggattcccga tctcgtgacg gcgaacgtcg gcgcgaacac gctttccatc
    13921 ttccccggcc agggctcgct cgcgttcggt acgcggatca atctggccgc ggccgttcgg
    13981 ccgcacaccg tcgtcgccaa ggatttgaac cacgacggcc acctggacct ggtggtctcg
    14041 cacatcggat ccaactcctt cggcgtgtgg ctgggcggcg gcaacggcac cttctcggcg
    14101 cggcacgact accagctcag cggctcgccg gtatacctgg gcgtctacga cttcaatgag
    14161 gatggaaacc tggacgtcgt ggcgaccgat cagacgcacg gcggcatcgg catcgcgctc
    14221 gggctcggcg acgggacgtt cggcgcggcg acgttcattc ccaccggaca gaacacgcac
    14281 gggctcgtgg gcgccgacct gaaccacgac gggcacatgg acatcgcggc caccaactcc
    14341 acgacgccgg gaaccgtgtc ggtgctcctg ggcaagggcg acggcacgtt ccagcccaag
    14401 atcgacttct ccacgagcag cccgcacacc gtgtccgccg gcgacctgga ccaggacggc
    14461 gacatcgacc tcgtcgtctg cgacttcgac acggggagga tcacgatcgc gctgggccac
    14521 ggggacggta cgttcgccac gctgccggag atcccgaccg gcacgaaccc gcacgcttcg
    14581 gcggtgggcg acgtggatgg cgacgggaag ctggacatcg tggtggcgaa ccaggcctcg
    14641 aacacgctct cgatgctgct cggccggggg gggccctcgt gggcgcccaa gatcgactac
    14701 cccgtgggcg ccggcgccca caacgtggcg atcgccgacc tggatgggga ccacctgccc
    14761 gaggtctcct gctccaacat cgtcgcgaac acggtcacga tcttgaagaa ccgcgggcac
    14821 gtgacgcatc cggcccgcgt gttcaccgcc ggaccgcgcg ccacgctgat gctccgcgcg
    14881 tccggtcccg actacgcgct ccacgtggag tcggcggacg gagccttcgc cgccaccgac
    14941 atcgacctcg cctcggtcga gctgcgctcc ccgggcacgg gcagcgtgga tcacattgag
    15001 gcggatgtcg cgaaggggat gacgaccgcg gatgccgatc agaacggcgt ggtggaggcg
    15061 gtgatcggct tcacgcgcgc cgatcttcgg aatcttttct ccggcgtggt cgggcgaaag
    15121 tcgatgacgg tgacgctgca gggacggctg gccgacgcca gctccttctc gggcacggcg
    15181 acgatcgatc tggtcggagc cggcgccccg ggcaagccgg tggcgaacgc gtcggtcgcg
    15241 ccgaacccgc tcaatcccgt cggcgtgctc accttcacgc tgacgcgctc cgaggcggtc
    15301 acggtgcgcc tgtacgacat ccgggggcgg ctggtgcggc ggtcgatgga gtcgaggccg
    15361 ctggatgcgg ggatctaccg gatcgcggtg gacggaacga cggaccaggg cggtccgctc
    15421 gcttcggggg tctacttctt cagaatcacg acccgggacg gagacgtctc gggacgcttc
    15481 gccgttctca aatgagcggc ggcggcgcag ctcgcgcacg gaccctcgcc cttgcgtttg
    15541 gggtcgcggg tccgcgcgaa gagccaggcg gcggcggcga gtgccaggcc caccgcgatc
    15601 acgtgctgca ggaccatgcc caggctcatc agccccatcc caggagccgc ccgccctgga
    15661 agaccatgaa cgaggcggcc cacgcgatcg cgttcatgaa gagcagcatg aagagaggcc
    15721 agcgccagga gttggtctcg cgccgcgcca cggcgaccgt ggacatgcac tggcaggcca
    15781 gcgcgaagaa aaccatgagg ctcaccgcga cgagcggcgt gtaggccagc gcgcccgtgc
    15841 ggtcgttgcg caccttgggc agcgtctgct tgagcgacat cgacgggtcg cccgacccgt
    15901 ccaggctgta gaccgtcgcc atcgtggaga tcatcacctc gcgcgccgcg accgaggaga
    15961 tcagccccac ccccatcttc cagtcgaagc cgagcggccg gatcaccggc tcgatcgcgc
    16021 ggcccagctg tcccgcgaag gaatggcgca gcgcctcgct cggcggcgtg cccggcttgc
    16081 tcaccggata ggaggcgagg aaccagagca ccagggagac cgcgacgatg acggtgcccg
    16141 ccttctggag gaagaggagg cagcggttcc ggatggtcat cgcgatcgat cgcagggacg
    16201 gcatgcggta gtgcggcagc tccatcacgt agagcggctt cggcccgcgg agcacggtgc
    16261 gcttcaagac cgcggccacg gcgagcgccg agaggatccc gaagagatag agcgagagca
    16321 gcgtcagccc cctcagattc aggatgccga gtacgaagcg gtcggggatg aacgcgccga
    16381 tcaggagtgc atagacggga agccgcgcgc tgcaggtcat gagcggcgcg atcatgatcg
    16441 tcgtgagccg gtcgttccgg ttctcgatgg tgcgcgtggc catgatcccc gggatcgcac
    16501 aggcgaagga cgagagaagc ggcaagaacg cccgtcccgg cagccccacc gcgcccatga
    16561 cgcggtccat gatgaaggcg gcgcgcgcca tgtagcccgt gtcctccatc agggcgatga
    16621 agaagaagag gatcgcgagc tgggggatga aggaggcgac cgtgcccgct cccgccagca
    16681 cgccgtccac caggagggag cggatcggcc cggccggcag cgtcgcctgc gccgcgcccg
    16741 cgagcgcggc gaagccggag gcgatcaggt ccatgaaggg ggaggcccag gcgtagatgc
    16801 tctggaacac ggcgcccatg aagagcacga ggatgatcgg tcccagcacc ttgtgcgtga
    16861 gtatccggtc gaggccggcg cgcggcgcct ggcgtccggg gacgcggcgg accgcggcct
    16921 tgatgatcgc ctcgacgctg gcgtagccca gggcgggatc cagcgcctgc tccagcgtga
    16981 ggcgatggcc gttgccgttg ccatggccgt tcacgtaccc gttcgcgtgc ccttccacgt
    17041 gcccgttgcc attcccgttc ccgttgccat tcccgttcac ggccgcgcgc gcgtccagcg
    17101 cggcgcgcag ccggtcgacg ccgtatcccg tcttggccga cacggcgagc accggaacgc
    17161 ccagcatccg ctccatcgcg gggacgtcga tcgcgatccc ctgggcctgg gcggcgtcga
    17221 tcaggttcag cacgaggacc gcgggacggc cggtcgcgag aatctgcagc gccaggtaca
    17281 gctggcgctc cagattcgtc gcgtccacga ccgcgaccac cagatcggcc cgctcctcgg
    17341 gggcgcaatg cccgtcgagc acgtcgcgca cgatcgcctc gtcgggggag cgcggcgcca
    17401 ggctgtaggt gcccgggaga tccacgatgt ccacgtgctt gcccgaggga agccggcagg
    17461 agccgacctt cttctcgacg gtgacgccgg ggtagttcgc gaccttctgg cgaaggcccg
    17521 tcaggcggtt gaagagggac gatttccccg agttggggct gccgacgatg gcgacgcgct
    17581 cagagggcat cgacatgaat ccgttccgcc tccgatcgcc ggatggagaa gtgcgatccg
    17641 cgcacggcca gctcgagcgg atcgccgaac ggggcgcgcc ggatcagcgc gacctcggtg
    17701 ccgggggtca gccccagctc catgagccgc atcgcgaatt ccttttccgc caggatgccc
    17761 cggaccacgg cgcgatcgcc gggggcgagc agcgcgagcg tgcgcgacgt catgacagcg
    17821 actcctttcc tccatccacg gggtcgatca gcgcttctaa agccacgcgc aggcagcggc
    17881 cgtggcagac ggggcagtgg gccgattccg ttccgctctc gcagcggttc tggtactcgc
    17941 ggaattcgtc gcggaaccgc tccgccgccg gatggtcgga gcgcaggaac gcggtgaggc
    18001 ggaggaagcg caccatcgaa tcggggctca ccagatgctc gatcatgcac gcgtcttcct
    18061 cggcggtcgc gggatcgagt cccaggatct cgccgaagaa ctcggtcagc acgtcgcggc
    18121 tccgctcggt gcgcgccgcg aggcgctgtc cttccacggt cagcgtcacg tcgccgtagg
    18181 gctcgtgctt ggccagcccg cgcgtcgtga gcgagcgcag catcgacgtc acgccggcct
    18241 tgctcacgcc gagccgctcc gcgatcgcgc tgaccgacgt gccgcccccg cgcgtcaagg
    18301 acgcgatcgc ctggagatag tgggccatcg agtgggtgag ctgcttttcg tgaaatcgct
    18361 tccaggtttc catgggcacg gcctccgggg ccggagcgac aaggcatttc ggtctgcgtg
    18421 gttaagtatg cttaactttc gtcgaaaagc ggctccaagt caacggtacc aaaggagtat
    18481 tctcccggac ccatgacgga acccaaggat acccagggcc ggtacctgga tcgggccatg
    18541 acctggctcg cattcaacgg gcgcgtgctg gaggaggcgc aggaccccca gagcgctatc
    18601 ttggagagag cgcgcttctt aggcatcttc gcgtccaatc tggacgagtt cttcatgatc
    18661 cgtgtggcct ccatccggga ccgggttcgc accggcgccg accagcgctc cgcggccggg
    18721 cagacgcagg ccgagcggct cacctccgtg caggcgcgcg cgcgcgagct gaccgccgcg
    18781 gcgtacacgc tctgggagca ggggatcgtt cccggacttc gggacgccgg catccggttc
    18841 ctgcgcgaat cggagctcga gcccaacgag cgcgcgttcg tggaccggct cttcatgcgg
    18901 gagctcctcc ccgtgctgac gccggcggcc gtcgccgagg acacgccctt tccgcgcctt
    18961 ttccacctgg cgctccatct cgcggtcctg ctcgccaagc ccggcgaaga ggagcggctg
    19021 gccgtggtgc agctgccgcg caccggcacg cggtgggtcc aggtgagcgg cggagcgcgc
    19081 gccgacggac agacgccggc gcagcgcttc atcctgatcg aggaggcggc gcgcgcgcac
    19141 ctgggggagc tcttcgccgg gtacgaggtg cgcgaggcgg tggccttccg cgtgacgcgc
    19201 gacgccgact tcgccaccga cgaccaggag gaggagaacc tggtggacgc ggtgcgccag
    19261 gtgctgcgcc agcggatgcg cggcgatccg gtgcggctgg agatcgcgcg cgaggcgagc
    19321 gacgccgtcg tggagcgcct ccggatcgcg ctggagctgg aggagcgcga ggtctaccgc
    19381 gtgtccgggc cgctcgactt ccgcttcctg atggatttcg cgcgccagcc ctcgctcaag
    19441 gcgccgcgcg ccccggcgtg gccgccgcag cccgtgcccg gcgtgccgcc cctggacgga
    19501 atctgggacg cgatccagga gcgggacatc ctcctcttcc atccctacga gacgttcgag
    19561 ccggtgctgc ggttgttggt gctcgcggcc gaggacccca gcgtgctggc gatcaagcag
    19621 gtgctctatc gcacgtcgtc cacgtcggaa gtggtgaacg cgctggagcg cgcggcggag
    19681 agcgggaagc aggtgacggt gctggtcgag ctgcaggcgc gcttcgacga ggagcggaac
    19741 gtgaactggg cgcgccgtct ggaggacgcc ggcgcgcagg tgctctacgg gctggcgggc
    19801 ttgaagacac acgccaaggc gcttctcatc gtccgccgcg gcccgggggg gatcgagcgc
    19861 tacgcccacg tcggcaccgg caactacaac gagcggacgg ccgccgagta ctccgacgtg
    19921 ggactcctct cggccgacga ggatctctgc gccgacctga ccggcttctt caacgtggtc
    19981 accggctact cggagccctt gccgtggcgc cggatcgaga tggcgcctct cgggctccgc
    20041 tcgcggctcc tcgcgttgat ccatcgcgag atcgcgaagg cgacgacgga agagccgggg
    20101 ctgatccgcg cgaagatgaa cgcgctgctg gacattccgc tcatcgaggc gctgtatgag
    20161 gcgtcgaacg cgggggtgcg gatcgacctc aacgtacgcg gctcgtgcct tctccgtccc
    20221 ggcgtgaagg ggatgtcgga gaacgtccgc gtcgtgagcc tggtggaccg gttcctggag
    20281 cacgcacgca tcttcgagtt ccgaaacggc ggcgacgtcg agaccttcat cgccagcgcc
    20341 gactggatgc cgcgcaacct ggaccggcgc gtcgagctca tgacgccgat cgtggatccg
    20401 gaacaccgcg cccggctggg ccgtattctg gacgcgatgt tcgccgacaa cgtgaaggcg
    20461 cgtgaattga ggtcggacgg atcgtacgtc aagcgcgacg ggaggaagaa ggcgacccgc
    20521 gcgcaggagc tgttctggga ggaggcccgc gcggccgctt cccggaccga ggatccggat
    20581 cgcagcgtgt tccagccctt gcggcgggct ccgaacgcgg gctgatccgg cgcgcggagc
    20641 gggcgcatcc ggcccgcctc cgctcatcgt gtcccactcg agggggaggg tcgcatggcg
    20701 agctgggtga accgcgtgat cggagcggcg cagctgcgct ccgcgacgta cgaggaagtc
    20761 gaggcggaca agtccgcgaa cctccaggcg ctcggggtcg tggtgctctc cgcgatcgcc
    20821 gcggggtttt ccatggcgca gggcggcgga cgcgcgatcg gcgtcgggat cctggccgcc
    20881 ctggtcgcgt gggtgctctg gtcggcgctc acctggctca tcgggacgaa gttcctgtcc
    20941 acgccggaga cccacgccga catcggcgag ctgctgcgca ccaccggctt cgcggcggct
    21001 ccggggatgc tccgcatctt cggcggcatc ccggtcgtcg gcttcgtctt caatctggtc
    21061 gcggggctct ggatgctggc ggccttcgtg gtcgcggtgc ggcaggcact ggactaccgg
    21121 agcacggggc gggccctctt ggtatgcggg atcggctggc tcgtctggtt cgccgtgatc
    21181 gcctggacgt tcctcctgct gggcgcttcg gtggccggat tccaggcgct gcggggaaac
    21241 atccaataga cgaaggtcag tagcgcacgt cgaacgccag ccgcgcgagc cattcgttcc
    21301 gctcgcgtcc ggcgacgtga ttctcgttcc gcacccgccc gtatcccgcg cccagcgaga
    21361 gatccacgtt gtcgcgcggc atccagcgcg cgtcgccgaa cacttcgcga cggcgctcca
    21421 cgatcccggt gagcgaggcg ttgctcaccg ggcccgtccc ctcgagaaag gcgtcgccca
    21481 gccttccctc gccgtggttg gtgaagtccc cgctccagcg gaactgccag tcgcggctcc
    21541 agtcgtagac cgcttcgacg aagacgttct ccacgtcggg acccagcgca aagcccagcg
    21601 ggcggtcccg gtagatgaag ttctgcccgt agtcgaccga gtaggtgaag ttccggacgc
    21661 gcgtgtactc gccgagcagg cggatcgcgc gcgcgccgaa cggccggtcg cttcgcagcc
    21721 cgagctgcca ggcgaagcgg tcgggcatgg tccggtcctc ggtggtgaag tcgtcgacga
    21781 gcagctcgcc cgtgagggag acgtcgcgcg tggggcgcca ggcgaggtcg gccgaggcca
    21841 tgacgttcgc gcgctccagc ccgcgcagcg agtcgctgga gacgtcgcgg atgtggatgc
    21901 gctccacgat ggcgtaggga aggatgccca gcgcgtagac caggtcgacg ttgtcgctcc
    21961 ggtaccgcac cgcctcgccc agtcccaccg tgagcgcgcg cgagacgttg gcctcgatcc
    22021 gatgcgcggc cagatagcgc cggtccacgc gcgatagctg ccccgtgacg gcggacgcgg
    22081 tcacgcgccc gcccaggcgc accgagtggt agaggaatcc catcggtccg gccgcgtccg
    22141 agaggagcag cgtcccgcgc cggcccggcc cccatcggaa gtcgtcgtag cccgccgcca
    22201 ccgtcagggc gcccgcgcgc gccgtgatct cggcgcggag gacggccgtc tccagatcgg
    22261 tgtgcgcgat gaccgcgtcg atccagtcgc gcgccgagcg gacccgcgtg acgcccagct
    22321 cctcgaaggc cgcgaacgcg ggccagagct ggagcgacat gcgcgcgctg gccgaggact
    22381 cgtcgcggaa gcggtagtgc gggtcgcgcc gctcctgata gtcgccgcgc aggtgcccgg
    22441 cggcggcgag gcggaaccgc tcttcgcgag ggcccgcgtc gaccaagggg cccgtctccg
    22501 gctcggccgg gggcgcgccc aggtccgtca gctcccgcgc cagctcgcgc tcgagccgct
    22561 ggaagtgaag gtccgcggcg atcgtggagt ccaggcgctc tgcccgcagc agcgcgcgcg
    22621 ccacgtcgat ccgcgcgagg ggccgcgaat acaccgcgat ggaatcgagc cgtcctcgcg
    22681 cggcgagcgc ctcgatctcc tcgtaggcga agtggcggac ggggaggaac tcggcgggct
    22741 ccgcccttaa cccgggcgcg atgccgccca gggcggcggc cagagcggcg accgccagca
    22801 atcccgcccg ggtgtcccgt ttcgttcgct cgctcatcgc cgtcccctcc cggtcaacgt
    22861 gccgccccgt tcccgcatcc aaccgaaggg gccgcgccag ggtagtggag cgggggcggc
    22921 gtatgtagaa agttcgacat agtgtgacat gaacgatgca gccgcgacac gggaccgcgc
    22981 tcctaaacgg cgcggcggca aagggttctt gaatggcaca tcgcctgctc ggaatgccgg
    23041 acgtctcacg gttctcacct ttcgcgaaag ggaggagtcc catgcagcgc accaccatca
    23101 tcaccgccgc catcttcgcg ggggccctgg ctactgcccc gtggagcgcc tccgcgggtc
    23161 ccctcgacta ccaggggctc gcgtttcaaa gccagggtag cggcgccaac tccgtcctgg
    23221 ccggagtgga gaccgggaac ggcgacgctg ttcccgtctt cgcctcgacc tcgtcgtcct
    23281 cgttcagcga gcgtcccatc taccggcgcc ggccctatcg cgatcgctat cgcgaccgct
    23341 acgacgacaa cgaccagccc cgctacttcg gcgcgttcgg cgccggcacg ttcgatccgg
    23401 atgaccagcc gggcaacggc ctctggctga acggcgagct gggcagcgaa gtcggcgacg
    23461 ccctcgatct cggcgttcgc gtgaactggt atcaccgcca gtcggacaat tcacaggtcg
    23521 tttccaccta caccgacgag gccggcaacg tccacgagcg ggtcctcgac acgaacaaca
    23581 tcgagacgaa cctcgttccg ctgatggcca ttctccgcgt ccgcttcccg gtttcccgcg
    23641 acttccagcc ctatgtgggc ggcggtatcg gatgggagtg gctcacggtg agcggcaccg
    23701 actcgaccgg cttcgacttc caggacgact acgatggatt cggcgggcag gtcttcgccg
    23761 ggctgaacgt cggcgtgtcg ccgaccttga acctctacgg cgaggcgctc tggaacaaga
    23821 gcaccgtgtc ggcgcgcttc ttcgatccgt tcgtgggcgc caccatcaag gacgagatcg
    23881 acatggacgg cctgggattg cacggaggac ttcgcttcag cttctgagac cacgctccga
    23941 tcttccggtt ctagtggccc ccgcgggcgc ggtgcggtac gtttccggct ggattccgcg
    24001 ggacgaaacg cgccgccccc gacgggggcg tcgtttggaa gcgaaccagg aggtgggatg
    24061 atggatcgga gccttacccg gagaacggtg cgggtcgccg cgctcggggt cgcgctggtc
    24121 gcgatcacgg gtctcgcggc gaccgcgccg cgcgcttggg cgcagacgtc cagcaccagc
    24181 gcgcgcacgg aagacgtttc gctcggcttc aagggaatgg gcgcccggat cggattggtc
    24241 gaccccgagg gcgcgagcag cacggtggac ctcggggttc acgtcgacgc cggcgagttc
    24301 gcccgcaata tccatctgca accgatcgtc gagtactgga gcgtggggca ggacgttacg
    24361 attctgagca gcacctattc cgtaaactct aaggatttct ccgtaggcgc agacatcaac
    24421 atcgattttc cgctgcagga cagccgggta actccctatg cgggtggcgg cctggggctg
    24481 cactggctga aggagaccaa tgacgctccc ggcggggcgg aattctcgga caccaagctc
    24541 ggtctgaatc tccagggcgg cgtccggacg gacgcgatgc cgaatctggc tctcttcggt
    24601 gaactgcgct ataacttcgt ctcggatgcc aatcagttga agatcctggg cgggtttacg
    24661 taccggttca tctactaggg acggcggagg agccatggac agcttgagac ggcttggaat
    24721 cgttgcattt tcgctggcgg tggtcgtggg cgtttcggcc tgtcagaaga agcagaccga
    24781 gacctcgacg tccgcgagcg acaccaccat gagcgcggaa agcccctatc ccgtcgagga
    24841 gacgccgccg gccgcgacca cgacgcctcc ggcggagaac ccgcccgcca ccagcgggac
    24901 caggaccacg acgcacacca ccacgccgaa gacgacccac tcgagcggca cgaccacggc
    24961 gtcgaaggtc acggtgcaca tccccgtggg cacgacgatg gacctcgtga tggtgaccga
    25021 cgcgagcacc aagacggcga acatcggcga tccgatcgaa gccaagctcg ccgccccgct
    25081 cgtcgtcgac ggcaaggtcg tggccgaaga gggctccgtg gtgcgcggca agatctcgga
    25141 cctgaagcgc gcgagccacg cgaaggacca ggaaggccgt gccttcctca agtacgactt
    25201 cacctcgatc gagaccgtgg gcggcacgaa gtcgctcaac gccaccgtga cgagcagcga
    25261 gggcaaggag ctggaggcca agtccacgag cacgcgtgac aagcttctca tcggcggcgg
    25321 cacggtcgcc ggcgcggtca tcggcaaggt cgccggcgga tccaccaaga gcacgatcat
    25381 cggcgccatc ggcggcgcgg ccgtcggagc cggtgccgtg gccgcctcga agggccacga
    25441 gctggagatc ccggcgggca gcaaggtcgt gctgaagacc gaggagccga tcacggtcat
    25501 gatggcgaag tagcgaacac cgcctcatcc ggaacgcaga cgcccccgtg ggaagcctcc
    25561 cgcgggggcg ttttgcctgg gatctccggc ccgggcgccg gccccggccg ccttgacaca
    25621 ccccgccccc agaccgatac tcgcgcccga cccacactta cagccgcgga tccaggagcg
    25681 acgaatggac gccagccagg cattgctgaa ggaattgtgc gagggcttcg gccctccggg
    25741 ccacgagacc gagatcgcgg ggatgatgaa gaagcacctg cgcggtctcg gggaagtgac
    25801 gcaggatggg ctcggaagca tcatctgcaa gaaaaaggga acggccgatc a
//
LOCUS       DASSLZ010000177        14502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_818038, whole genome shotgun sequence.
ACCESSION   DASSLZ010000177 DASSLZ010000000
VERSION     DASSLZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14502
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818038"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2198)
                     /gene="carB"
                     /locus_tag="VFM00_06925"
     CDS             complement(<1..2198)
                     /gene="carB"
                     /locus_tag="VFM00_06925"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET7498016.1"
                     /translation="MPRDPSIQSVLVIGSGPIVIGQACEFDYSGTQAVKALRGEGIRV
                     SLVNSNPATIMTDPEYADRTYVEPLTPEVLEAILERERPDAVLPTVGGQTALNLAVTL
                     SERGVFERLGIRLIGASRRAVEVGEDRRLFKEAMARIGLDVPKSRFARTLEEARSALA
                     VTGLPAVIRPSFTLGGTGGGIAYNEEEFEETATRGLDLSPVHEVLIEESVIGWKEYEL
                     EVMRDRADNFVVVCSIENFDPMGIHTGDSVTVAPAQTLTDREYQVMRDAARAIIREVG
                     VETGGSNIQFAVDPATGRLVVIEMNPRVSRSSALASKATGFPIAKIAALLAIGYRLDE
                     IKNDITRVTPASFEPVLDYVVVKIPRWAFEKFRDASPRLGTQMKSVGEVMAIGRTFKE
                     ALQKGLRGLETGSGGFGGRELDDARIREKLLTPDPDRILYVKRALEQGWTHEEIHRMT
                     GIDTWFLDQIQQIADLKEHLAGPLDSAALREAKRMGFSDARIASLSGTTEAGVRALRE
                     ELGIRPVYKRVDTCAAEFESHTPYLYSTYEEECEAAPTDRPKVMILGSGPNRIGQGIE
                     FDYCCCHGSFAFREEGFETIMVNCNPETVSTDYDTSDRLYFEPLTFEDVMNVIEREKP
                     EGVVIQFGGQTPLRLALPLHRAGVPILGTSPDAIDLAEDRRRFSTLLDELGIPQPESG
                     TATSLEEAKEVAARVGYPVLVRPSYVLGGRAMAIVYAEAHLEQYVREAVRT"
     gene            complement(2200..3369)
                     /gene="carA"
                     /locus_tag="VFM00_06930"
     CDS             complement(2200..3369)
                     /gene="carA"
                     /locus_tag="VFM00_06930"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET7498017.1"
                     /translation="MTHARRPAVLALEDGIVFFGNSFGRIGETSGEVVFNTALCGYQE
                     VLTDPSYRGQIVTMTYPHIGNYGINHEDVESARPQVAGFVVREISTAASSWRASGELH
                     AYLDEAGIVGIAEIDTRKLTRHIRSRGACRGVISALDLDPASLVKKAQSAAAMIGADL
                     AREVTCAEPYRYEPARDGGAAKDGVERPFHVVAYDLGMKRNILRLLTDAGCTVTVVPA
                     ATTAAEALALDPEGIFLSNGPGDPEACDYAVEASRGLIASGRPVFGICLGHQILGLAC
                     GGKTFKLKYGHRGGNHPVKNVATGQVEITAQNHGFAVDMSLFEDRAYLLTHVNLNDGT
                     VEGFRHRDLPVFSVQYHPEASPGPHDSHYLFRDFTRAMAERRAVGATGPLLQGGA"
     gene            3559..4149
                     /gene="msrB"
                     /locus_tag="VFM00_06935"
     CDS             3559..4149
                     /gene="msrB"
                     /locus_tag="VFM00_06935"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7498018.1"
                     /translation="MRSRNPIPFLAATILAATMSIPIAASSAKAGSAGSPKPVAAKQA
                     VARRDTGAIRVYSASKGGFVMTEKVTKSDAEWKKQLTPEQYQVTRKAGTERAFANAYW
                     NNHDKGIYRCVACGNDLFTSETKFDSGTGWPSFWKPIALENIEVGTDMTLGIARDEVK
                     CARCGSHLGHVFDDGPKPTGLRYCMNSAALQFVKKG"
     gene            complement(4172..4810)
                     /locus_tag="VFM00_06940"
     CDS             complement(4172..4810)
                     /locus_tag="VFM00_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016855764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET7498019.1"
                     /translation="MFRDREEAARLLAAKLSKYRGSRPLVLGSPRGAVVMAAIIADRL
                     GGDLDVALVRKLGAPGQPELAVGAVDETGRVTLDGTPDELGLDAAYVERERERQAAVL
                     RSRRARYTPARAPVDPSGRTVIVVDDGIATGATLAAALRLVRSRGPARLVAAAAVAPP
                     PAAARLAREADEVVVLATPEPFVAVGAFFDDFRQVSDDEVVELLERFGSDEG"
     gene            4903..5340
                     /locus_tag="VFM00_06945"
     CDS             4903..5340
                     /locus_tag="VFM00_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7498020.1"
                     /translation="MNVLVAVDDSPFSNAALEYVKRFTWPPDTEFLVVSACPPVWLGP
                     GEAVAADAIVELNRHQESYHRETADKGAAQLQAAKLRAKAKLLQGDPRHALLDEARAS
                     KADLIVVGSHGRSGVAKLLLGSVASHVVSHAPCSVLVVRSGAK"
     gene            complement(5355..6551)
                     /locus_tag="VFM00_06950"
     CDS             complement(5355..6551)
                     /locus_tag="VFM00_06950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7498021.1"
                     /translation="MSDRRILYAAAFLRALATGMIGVLIGIYLARQGFDPAGIGAVVT
                     AGLAGAATAALLVTVGGDRAGRKRTLLWLAILGAAGGAVVAVAAKPLVIAAAAFFGML
                     NGMGRDRGASMILDQAILPATAAPENRTAVFAWYNVVQDAGHALGALAAGVPALFRAG
                     HLSADGSMRAAVGIYALLLAVTALLYTRLGREVEAEAPAARVRLSPETRRIVTRVSAL
                     FSLDALGGGFLTSALLSYFFYARFGVREEGIGALFFGARLLNAASHVGAAWLAKRIGL
                     VNTMVFTHIPSSLLLLTVPFAPTFTIAAVLFLLREGLVEMDVPTRQSYVMAVVRPEER
                     TAASGVTHLVRLAMWAVAPAFAGLFMRGGSLGAPLMIGAGMKIAYDGLLYAAFRHVRP
                     PEERKA"
     gene            complement(6583..7593)
                     /locus_tag="VFM00_06955"
     CDS             complement(6583..7593)
                     /locus_tag="VFM00_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET7498022.1"
                     /translation="MSAPFGLDSLSTYALVIIGLAIVFDFLNGFHDAANSIATVVSTR
                     VLTPGFAVIWAAFFNFVAVFVLGEAVAKTVGKGIVTAESVNNHVLLSALVGAIVWNLL
                     TWWLGLPTSSSHALIGGLVGAALCFGGWRAIVIPGLTKTAIFIVIAPLLGLVLGLFNM
                     TATSWLFRRSAPAKVDRLFRRLQLVSAAAYSLGHGGNDAQKTMGVIALLLFTTGATDH
                     FYIPNWVVIVCGLAIALGTLSGGWRIVHTLGARVTKLHPFGGFAAETAGAISLFLATH
                     LGIPVSTTHTITGAIVGVGATRRLSAVRWGIAGQIVWAWVLTIPAAAAVAAFTYFVTS
                     RF"
     gene            complement(7601..8212)
                     /locus_tag="VFM00_06960"
     CDS             complement(7601..8212)
                     /locus_tag="VFM00_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HET7498023.1"
                     /translation="MRLLPTTPKFFELFERQSATLLLGARALRDLAENFDDVEAKAHR
                     IKEIESDGDQITHDLIDSLNRTFITPFDREDIHALASALDDVLDNIEGVASRFALFRV
                     RELTPETRELILIIERACVAIHEAVKCLRNRKEVQKNLVEINKLENDADMISRDMTAK
                     LFETAADFRELIKWKELYGRLEATTDDCEDVANIIEGIFVKNT"
     gene            8338..9399
                     /locus_tag="VFM00_06965"
     CDS             8338..9399
                     /locus_tag="VFM00_06965"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET7498024.1"
                     /translation="MPRRRLKSLLRWLPGTLILAGLAVVALRKVEWTHFSEIVKRAEP
                     AWLVAAVLMQLGTYLCAAATLQRGIKASGVSRNLFGLVPLGLAKLFVDQVVPTGGLGG
                     TVLVVRALERRRVGRGVATAAVVVNTLAFYASYAIAVGGALAVLWMRHGISRAILFLL
                     TLFAIYAVAMPVLILWIVYGGRRSHPRWLAKIPGLEKAISAMEDAPTSTLRHPMLFLE
                     SILLQLGIIVLDAWTLDALLRSIGHPAPFLVLFTSFVLASIAATLSLLPGGVGSFEAG
                     SVASMRYLGIPLEVALTGTLLFRGLTLWLPMLPGIWFARHEMMGEAETNTRSGREREE
                     PKDVAARGRQRRTAGVKRR"
     gene            9464..11671
                     /locus_tag="VFM00_06970"
     CDS             9464..11671
                     /locus_tag="VFM00_06970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET7498025.1"
                     /translation="MTAQGARGVPRPLRPFPQHPDETTRSDRIIEEGFMYRRPHRRHH
                     LTALAALAMAALLPALAPAPAAAAKKSAVPAFFYSDKPSVATYKAESEAELKQAQAAL
                     DRMVGVKGKRTLENTLGPYNEMMMHAENVAYQANLMESVHPDSVFRAEAENQTRVSAK
                     FLDDVSLNRQVYDALKAVDVSKADAATRYFYDRQIQDFRRSGVDKDDATRKQIADLLQ
                     HLTQVGQEFDKNIRNDSRFILLDKADDMAGLPEDFVKSHQPGPDGKIKISIEYPDLFP
                     VIRYAKSGDVRRRLMFEDQNRGYPANIAVLDSMIADRYQLAKILGYPNWAEYITENKM
                     IGNAQNASDFIQRLNDLTMKRASDEYAMYLKRKQEDDPTATAVNRWEVSYYGRLIRKR
                     DYDFDPQAMRPYFAFANVEKGVLDVTSKMFGVTYKKANVPVWDPSVEAYEVYDGSKLL
                     GRFFFDLHPRPGKYNHAAKFGIRQGVAGVQVPEAALVCNFPGGTPGDPGLMEQSDVET
                     FFHEFGHLIHGIFGGQGRWEPVSGTATERDFVEAPSQLLEEWPQDAKVLQTFAKHYQT
                     GQPIPADMVEKMRRADAFGRAMSYATQTFYSAVSLNIYNRPASEVNTDAIVAAAEPKY
                     TPVPSMPGTHMQCSFGHLDGYSAVYYTYEWSLVIAKDMFSAFNKSNLLDPTIARRYRD
                     EVLRPGGSRPARESVKAFLGRDYDFKAFDKWLAGEETAQAPAAESSAPASAGH"
     gene            complement(11693..13183)
                     /locus_tag="VFM00_06975"
     CDS             complement(11693..13183)
                     /locus_tag="VFM00_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7498026.1"
                     /translation="MTAPESGGSAGAAPMTAAPGPRRSLFWTIAGMFFLTTVGGSLLQ
                     IFVAVGVLRPLETREARSRAELGAASAATEIAALPEPLTDAKVHVVLASARDRMDFRG
                     SSIVYRGADGMYTSEPLTPPRFRAPGSDSLAFGGARGRDRDRGRPRFDVLAERPVRRG
                     ASVIGTVQVRRWQPRSGWGYWEMRTALLFVPFAVLASGLAGLVIVRLLVRRLRAIDAL
                     ATRVAQGDLSVRIQDRSGDEIGRIAEQLDRMTERIAEARASLERNEKQRRQLFADITH
                     ELATPLTSIRGFAETLLDPAVGVSADERAKYTRGLLEEAARLDRLISDLFDLARLEAG
                     ASALHLERLDLAALAGHTVERYQKRCGDSGLRLEWEPPAGPAWIEADGRRMEQVLENL
                     LNNAIRYVPRGGSVRVSVDPSGSRLGATHSLRVEDDGPGVPRGEMGQVFERFYRAESA
                     GHGSDGKGSGLGLAIVKEIVERHQGTVRAEPIDPHGLSIVVELPPA"
     gene            complement(13180..13866)
                     /locus_tag="VFM00_06980"
     CDS             complement(13180..13866)
                     /locus_tag="VFM00_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019055311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7498027.1"
                     /translation="MSQKILIVEDDSRIASLIVKNLEAAGYECEVSADGGHAVAAFAR
                     QKPALVVLDLGLAGLDGLEVARRIRKESDTPILMVTARSGESDKLLGFEIGADDYVTK
                     PFSTAELVARVRVLLRRSSGAVGDRLLEVGGLKIDPARRTVERDGVAVPLTTLEFDLL
                     YLMASRPGRVFSREALMEQVWGTDRVVDDRSIDSLVSRLRRKLEADASNPRYLQTVWG
                     AGYRIAEPAS"
     gene            14173..>14502
                     /locus_tag="VFM00_06985"
     CDS             14173..>14502
                     /locus_tag="VFM00_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7498028.1"
                     /translation="MRTLRWLLPLLLAAAPAGAMAEPRPITLQEAVRLARENAPAVVQ
                     ARGQERSADAAKRSAYAAFLPSLSLSAGATRRYPAGGTRVDPQTQQVISSGDPWGYST
                     GLTASVDL"
BASE COUNT         2290 a         5148 c         4935 g         2129 t
ORIGIN      
        1 gtgcgcaccg cctcgcggac gtactgctcg agatgcgcct cggcgtagac gatcgccatg
       61 gcgcggccgc ccaggacgta cgaggggcgc accagcacgg gatagcccac gcgggcggcg
      121 acctccttcg cctcttccag cgacgtggcc gtgccgctct cgggctgcgg aatgccgagc
      181 tcgtcgagaa gggtgctgaa gcggcgccgg tcctcggcca ggtcgatcgc gtcggggctg
      241 gtgcccagga tcggcacgcc ggcgcgatgg agcggcagcg ccagccggag cggcgtctgc
      301 ccaccgaact ggatcaccac cccctcgggc ttctcgcgct cgatcacgtt catcacgtcc
      361 tcgaacgtga gcggctcgaa gtagaggcgg tccgacgtgt cgtagtcggt ggagacggtc
      421 tcggggttgc agttcaccat gatcgtctcg aacccttcct cgcggaaggc gaacgagccg
      481 tggcagcagc agtagtcgaa ctcgatcccc tgcccgatcc ggttgggccc cgagcccagg
      541 atcatcacct tgggccggtc ggtgggcgcg gcctcgcact cctcttcgta ggtcgagtac
      601 aggtagggcg tgtgtgactc gaactcggcg gcacaggtat ccacgcgctt gtagacgggg
      661 cggatgccca gctcctcgcg cagggcgcgc accccagcct ccgtggttcc cgacagcgag
      721 gcgatcctcg catccgaaaa gcccattcgc ttcgcctcgc gaagcgccgc cgaatccaaa
      781 ggcccggcca ggtgctcttt caggtccgcg atctgctgga tctggtcgag gaaccacgtg
      841 tcgatccccg tcatccggtg gatctcctcg tgggtccacc cctgctccag ggcgcgcttc
      901 acgtagagga tccggtcggg atcgggcgtc agcagcttct cgcggatgcg cgcgtcgtcc
      961 agctcgcggc cgccgaagcc gccgcttccg gtctccagcc cgcgcagccc cttctgcagc
     1021 gcctccttga aggtgcggcc gatcgccatc acctcgccca ccgacttcat ctgcgtcccc
     1081 aggcgcgggc tcgcgtcgcg gaacttctcg aacgcccagc gcgggatctt caccacgacg
     1141 tagtcgagca ccggctcgaa ggaggccggg gtcacgcggg tgatgtcgtt cttgatctcg
     1201 tccagccggt agccgatcgc gagcagtgcc gcgatcttgg cgatcgggaa gcccgtcgcc
     1261 ttggacgcca gcgccgagga gcgcgacacg cgcgggttca tctcgatcac caccagccgc
     1321 ccggtcgcgg ggtcgaccgc gaactggatg ttcgagccgc cggtctcgac cccgacctcg
     1381 cggatgatcg cccgcgcggc gtcgcgcatg acctggtact cgcggtcggt cagggtctgc
     1441 gccggcgcca cggtcaccga atcgccggtg tggatcccca tcggatcgaa gttctcgatg
     1501 gagcagacga ccacgaagtt gtccgcgcgg tcgcgcatca cctcgagctc atattccttc
     1561 cagccgatga ccgattcctc gatcagcacc tcgtgcaccg gggagagatc caggccgcgc
     1621 gtcgcggtct cctcgaactc ctcctcgttg taggcgatcc cgcccccggt gccccccagg
     1681 gtgaaggagg ggcggatcac ggccgggagc ccggtcacgg ccagcgccga tcgcgcctcc
     1741 tccagcgtgc gcgcgaagcg gctcttcggc acgtccagac cgatgcgcgc catcgcctcc
     1801 ttgaagaggc gccggtcctc gcccacctcg accgcgcggc gactcgcgcc gatcaggcgg
     1861 atgccgaggc gctcgaagac gccgcgctcg gacagcgtca ccgccaggtt cagcgcggtc
     1921 tgccccccca ccgtgggcag cacggcgtcg gggcgctcgc gctcgaggat cgcttccagc
     1981 acctcggggg tgagcggctc gacgtacgtg cggtcggcgt actcggggtc ggtcatgatc
     2041 gtggcggggt tggaattcac cagcgagacg cggatcccct cgccgcgaag cgccttcacc
     2101 gcctgggtgc cggagtagtc gaactcgcac gcctgcccga tgacgatcgg acccgagccg
     2161 atcaccagca ccgactggat cgagggatcg cgcggcatgc taggcgcccc cctggagcag
     2221 cggtcccgtc gcgccgacgg cgcgccgctc ggccatcgcc ctcgtgaaat cgcggaagag
     2281 gtagtggctg tcgtgcggcc cggggctggc ctcggggtgg tactggaccg agaagacggg
     2341 gagatcgcgg tggcggaagc cctcgacggt gccgtcgttc aggttcacgt gcgtgagcag
     2401 gtaggcgcga tcctcgaaca gagacatgtc cacggcgaag ccgtggttct gcgcggtgat
     2461 ctccacctgc cccgtggcga cgttcttgac cggatggttc ccgccccggt gcccgtactt
     2521 gagcttgaag gtcttcccgc cgcaggcgag gcccaggatc tggtgcccca ggcagatgcc
     2581 gaacaccggg cgccccgacg cgatcagccc gcgcgacgcc tccacggcgt agtcgcacgc
     2641 ctcgggatcg ccgggcccgt tggagaggaa gatcccttcc gggtcgagcg ccagcgcctc
     2701 ggcggcggtc gtcgccgccg ggaccacggt gaccgtgcac cccgcgtcgg tgagcaggcg
     2761 gaggatgttc cgcttcatgc cgaggtcgta ggccacgaca tggaaggggc gctcgacgcc
     2821 gtcctttgcc gcgccgccgt cgcgagccgg ctcgtagcga tagggctccg cgcaggtgac
     2881 ttcgcgcgcg agatcggcgc cgatcatggc cgccgcgctc tgcgccttct tcaccagtga
     2941 cgccggatcg aggtcgagcg ccgagatcac gccgcggcag gcgccgcggc ttcggatgtg
     3001 gcgcgtcagc ttgcgcgtgt cgatctccgc gatgccgacg attcccgcct cgtcgagata
     3061 ggcgtgcagc tcgcccgagg cgcgccagga gctggccgcg gtggagatct cgcgcacgac
     3121 gaatcccgcg acctgcgggc gcgccgactc gacgtcctcg tggttgatgc cgtagttgcc
     3181 gatgtgcggg taggtcatcg tgacgatctg cccgcgatag gaaggatcgg tgaggacttc
     3241 ctggtaaccg cagagcgcgg tgttgaaaac gacttcaccc gacgtctcac cgatccgccc
     3301 gaagctgttg ccgaaaaaga cgatgccgtc ctcgagggcg agcacagccg gacggcgggc
     3361 gtgggtcatc gggtgccgat ggtctccttt cgggagcgga tcgcgcggga gcgggatccg
     3421 cgctgcgcgg aaatctatcg cacgttggat gcgcccgcaa tggcgcagga gcggccggcg
     3481 ccgatttttc gttccggcgg cctccagcct gtcaccgcgc ggcggcgcgg acgtatcctt
     3541 ggcgaaagga ggagcacgat gcgaagccga aacccgatcc cgttcctcgc cgccacgatc
     3601 ctggcggcca cgatgtcgat cccgatcgcc gcctcgtccg caaaggccgg gtcggcgggt
     3661 tctcccaagc ccgtcgcggc caaacaggcc gtcgcgcggc gcgacacggg agccatccgc
     3721 gtctattccg cctccaaggg agggttcgtc atgaccgaga aggtgaccaa gtcggacgcc
     3781 gagtggaaga agcaactcac gcccgagcag taccaggtca cgcgcaaggc cggcaccgag
     3841 cgcgccttcg cgaacgccta ctggaacaac cacgacaagg ggatctaccg ctgcgtcgcc
     3901 tgcggcaacg acctcttcac gtcggagacc aagttcgact cgggcaccgg ctggccctcg
     3961 ttctggaagc cgatcgccct ggagaacatc gaggtgggaa cggacatgac cctgggcatc
     4021 gcccgcgacg aggtgaagtg cgcgcggtgc ggctcgcacc tgggacatgt gttcgacgac
     4081 ggccccaagc cgaccgggct ccgctactgc atgaactcgg cggcactgca gttcgtgaag
     4141 aaggggtagc cccggaaggg gcttccccgc ttcacccctc atcactcccg aaacgctcga
     4201 gcagctcgac cacctcgtcg tcgctcacct gccggaagtc gtcgaagaag gctccgaccg
     4261 ccacgaaggg ctcgggcgtg gcgagcacca ccacctcgtc cgcctcgcgc gcgagccggg
     4321 ccgccgccgg cggcggcgcg accgcggccg ccgccaccag ccgcgcgggc ccgcggcttc
     4381 gcaccaggcg aagcgccgcc gcgagcgtgg cccctgtcgc gatcccatcg tccaccacga
     4441 tcacggtgcg cccggaggga tccaccggcg cgcgggccgg cgtgtaacgc gctcgccgcg
     4501 accggagcac cgcggcctgc cgttcccgct cccgctccac gtaggccgcg tccagcccga
     4561 gctcatcggg ggtaccgtcc agggtcacgc ggcccgtctc gtccaccgcg cccaccgcga
     4621 gctcgggctg ccccggcgcg cccagcttcc gcaccagcgc gacgtccaga tcgcctccca
     4681 gccggtcggc gatgatggcg gccatgacca cggcgccccg gggactgccc aaaaccagcg
     4741 gtctcgatcc ccggtacttc gagagcttcg cggcaagcag ccgcgccgcc tcctcccggt
     4801 cacggaacat gcctccaagg taccccttcc ggagggctcc cggacaaggt accgtcgcct
     4861 cgtcttatca agcgaaccgg agcctggctc gcaggaggtc cgatgaacgt tctcgttgcg
     4921 gtcgacgact cgcccttttc caacgcggcg ctggagtacg tgaaacgctt cacgtggccc
     4981 cccgatacgg agttcctcgt ggtctcggcc tgcccccccg tctggctcgg gccgggcgag
     5041 gcggtggcag ccgacgcgat cgtggagctg aaccggcatc aagaaagcta tcaccgggag
     5101 acggccgaca agggcgcggc ccagctccag gccgcgaagc tccgcgccaa ggccaagctg
     5161 ctccagggcg atccgcggca tgccctgctg gacgaggcgc gcgcgtcgaa ggcggacctg
     5221 atcgtggtgg gatcgcacgg gcgctccggc gtggcgaagc tcctcctggg aagcgtcgcg
     5281 tcgcacgtgg tgtcgcacgc gccctgcagc gtgctcgtgg tccggtccgg agccaagtaa
     5341 gccgcgactc gccgctacgc ctttcgctcc tccggcggac gcacgtggcg gaacgccgcg
     5401 tagagcaggc cgtcgtaggc gatcttcatc cccgccccga tcatgagcgg cgctccgagt
     5461 gagcctccgc gcatgaaaag tcccgcgaac gccggcgcca cggcccacat cgccagccga
     5521 accagatgcg tcacccccga cgccgccgtc cgctcctccg gccgcacgac cgccatcacg
     5581 tacgactgcc gggtgggaac gtccatctcc acgagcccct cgcgcagcag gaagagaacg
     5641 gccgcgatcg tgaacgtggg ggcgaacggc accgtgagga gaaggaggct cgaggggatg
     5701 tgcgtgaaga ccatcgtgtt caccagcccg atccgcttcg cgagccaggc cgcgcccacg
     5761 tgcgaggccg cgttcaggag tcgcgccccg aagaagagcg cgccgatccc ctcttcccga
     5821 acgccgaagc gggcgtagaa gaagtaggag aggagcgcgc tcgtcaagaa tcccccgccg
     5881 agcgcatcca gcgagaagag cgccgagacc cgcgtgacga tgcgccgcgt ctcgggcgag
     5941 aggcggaccc gcgccgcggg agcctccgcc tcgacctcgc gtcccagccg cgtgtagagg
     6001 agcgccgtga ccgcgaggag gagcgcgtag attccgaccg cggcgcgcat cgaaccgtcc
     6061 gcggagaggt ggccggcgcg aaagagggcc ggaacccccg cggccaaggc acccagcgcg
     6121 tgtcccgcgt cctgaacgac gttgtaccag gcgaacaccg ccgtgcggtt ctcgggagcg
     6181 gccgtggcgg gaaggatcgc ctggtccagg atcatcgagg cgccgcggtc gcggcccatc
     6241 ccgttcagca ttccgaagaa ggccgcggcg gcgatcacga gcggcttcgc ggccacggcc
     6301 acgacggcgc ccccggcggc gccgaggatg gcgagccaaa ggagcgtgcg cttgcggccg
     6361 gcgcgatcgc ctcccacggt gacgaggagc gccgcggtcg ccgcgccggc gagcccggcg
     6421 gtcacgaccg cgccgatccc ggccggatcg aagccttggc gcgcgaggta gatcccgatc
     6481 agcacaccga tcatccccgt cgcgagggca cgaaggaagg cggcggcgta aaggattcgg
     6541 cggtcggaca cggctagagc cccgcggccg gaggcgccac ggctagaagc gcgaggtgac
     6601 gaagtaggtg aaggcggcga ccgccgcggc ggccgggatg gtgagcaccc aggcccagac
     6661 gatctgcccc gcgatgcccc agcggaccgc cgagagccgg cgcgtcgcgc cgacgccgac
     6721 gatggcgccg gtgatcgtgt gcgtcgtgct gaccggaatg cccaggtggg tcgcgaggaa
     6781 gaggctgatc gcgcccgccg tctcggccgc gaacccgccg aaggggtgca gcttggtcac
     6841 ccgcgctccc agcgtgtgga cgatgcgcca gccgcccgaa agggtgccga gcgcgatcgc
     6901 cagcccgcag acgatcacca cccagttggg gatgtagaag tggtcggtgg cgccggtcgt
     6961 gaagaggagc agggcgatca cccccatcgt cttctgagcg tcgttccccc cgtggcccag
     7021 cgagtaggcg gccgcggaga ccagctggag gcgccggaac aatcggtcca ctttcgcggg
     7081 agcgcttcgt cggaagagcc atgacgtggc cgtcatgttg aaaagcccga ggaccaggcc
     7141 caggagcgga gcgatgacga tgaagatcgc cgtcttggtg aggcctggga tcacgatggc
     7201 gcgccagccg ccgaaacaga gggccgcgcc caccaggccg ccgatcagcg cgtgcgagga
     7261 ggaggtcggg agcccgagcc accaggtgag gaggttccag acgatcgcgc ccaccagggc
     7321 cgagaggagc acgtggttgt tcaccgactc ggcggtgacg atccccttcc ccaccgtctt
     7381 cgccacggcc tcgcccagca cgaacaccgc cacgaaattg aagaaggccg cccagatcac
     7441 ggcgaatccc ggcgtgagga cgcgcgtgga gacgacggtg gcgatggagt tggcggcgtc
     7501 gtggaagccg ttcaggaagt cgaagacgat ggccagtccg atgatgacca gcgcgtaggt
     7561 ggagagcgaa tcgaggccga acggtgcgct cacggtccgt tcaggtgttc ttgacgaaga
     7621 tgccttcgat gatgttggcc acgtcttcgc agtcgtccgt ggtggcctcc aggcgcccgt
     7681 acagctcctt ccacttgatc agctcccgga aatccgcggc cgtctcgaat agtttggccg
     7741 tcatgtcgcg ggagatcatg tcggcgtcgt tctccagctt gttgatctcg accaggttct
     7801 tctgaacttc cttgcggttc cggagacact tcacggcctc gtggatggcc acgcacgccc
     7861 gctcgatgat caggatcagc tcgcgcgtct ccggcgtcag ctcgcgcacg cggaagagcg
     7921 cgaaccggct ggcgacccct tcgatgttgt cgagcacgtc gtcgagcgca ctcgccaggg
     7981 cgtggatgtc ttcccggtcg aagggggtga tgaaggtccg gttcaggctg tcgatgaggt
     8041 cgtgcgtgat ctggtccccg tcgctctcga tctccttgat ccggtgcgcc ttcgcctcca
     8101 cgtcgtcgaa gttctccgcc aggtcccgga gcgcgcgcgc gcccaggagg agcgtcgcgg
     8161 actggcgctc gaagagctcg aagaacttgg gcgtggtcgg aagcaggcgc atggcgactg
     8221 ggtatacaac gtccgggcgc cgcctgtcca cgacccagcg ctcccccccc ggccgggggc
     8281 ctccccaccg gcgttccggc ttggacccgg ccgcccctgc ctgtaggatc gccctccatg
     8341 ccgcgacgcc gcctcaaatc gctccttcgc tggcttccgg gcaccctgat cctcgccggg
     8401 ctcgccgtcg tcgcgcttcg aaaggtcgag tggacccact tttccgagat cgtgaagcgg
     8461 gcggaacccg cctggctggt ggcggccgtg ctgatgcagc tgggcaccta tctctgcgcc
     8521 gcggccaccc tgcagcgggg gatcaaggcc tccggcgtga gccggaacct gttcgggctg
     8581 gttccgctcg gcctggccaa gctcttcgtg gatcaggtcg tccccacggg cggactgggg
     8641 ggaaccgtgc tcgtcgtgcg cgcgctggag cgccgccgcg tggggcgcgg cgtggcgacc
     8701 gccgccgtcg tcgtgaacac cctcgcgttc tacgcctcct acgcgatcgc cgtgggcgga
     8761 gcgctcgcgg tcctctggat gcggcacggc atctcgcgcg cgatcctctt cctgctgacg
     8821 ctcttcgcga tctacgccgt ggcgatgccg gtcctgatcc tgtggatcgt ctacggcgga
     8881 cggcgctcgc atccgcgctg gctggcgaag atcccgggcc tggaaaaggc gatctccgcc
     8941 atggaggacg cgccgacgtc caccctgcgc cacccgatgc tcttcctcga atcgatcctc
     9001 ctgcagctgg gcatcatcgt gctcgacgcc tggacgctcg acgcgctcct tcgctccatc
     9061 gggcaccccg cccccttcct ggtcctcttc acgagcttcg tgctcgcctc gatcgccgcc
     9121 acgctgagcc tgcttccggg cggcgtcggc tcgttcgagg ccggatcggt ggcgtcgatg
     9181 cgctacctgg ggatcccgct ggaggtggcc ctgacgggga cgctcctctt ccgtgggctg
     9241 accctctggc tgcccatgct ccccggcatc tggttcgccc gtcacgagat gatgggcgag
     9301 gccgagacga acacccggag cggccgggag cgggaggagc ccaaagacgt ggccgcccgg
     9361 gggcggcagc ggcggaccgc gggggtcaag cgacgctgag accttgacga cgcatcgact
     9421 tccaacgaac cggaagcgcg ccccatctgt actagggtat aaggtgacgg ctcagggcgc
     9481 ccgcggcgtg ccgcggccgc tccgcccctt cccgcagcac cccgacgaaa cgacacgaag
     9541 cgaccggatc atcgaggagg gtttcatgta ccgacgtccg caccgacggc accacctgac
     9601 cgcgctcgcc gcgctcgcga tggcggcgct tctcccggct ctggcgccgg cccccgcggc
     9661 cgcggccaag aaatccgccg tgccggcctt cttctacagc gacaagccct cggtggccac
     9721 gtacaaggcc gagtcggagg cggagctcaa gcaggcccag gccgcgctgg acaggatggt
     9781 cggcgtgaag ggcaagcgca ccctcgagaa cacgctcggt ccctacaacg agatgatgat
     9841 gcacgccgag aacgtcgcct atcaggcgaa cctcatggag tcggtccatc ccgactcggt
     9901 gttccgcgcc gaggcggaga atcagacccg ggtctcggcc aagttcctcg acgacgtcag
     9961 cttgaaccgg caggtctacg acgcgctgaa ggcggtggac gtatccaagg ccgacgcggc
    10021 gacgagatat ttctacgacc gccagatcca ggacttccgc cgctcgggcg tggacaagga
    10081 cgacgcgacc cgcaagcaga tcgccgatct cctccagcac ctcacccagg tcgggcagga
    10141 gttcgacaag aacatccgga acgactcccg cttcatcctc ctcgacaagg cggacgacat
    10201 ggccggcctg cccgaggact tcgtgaagtc gcaccagccc ggcccggacg ggaagatcaa
    10261 gatctcgatc gagtaccccg acctcttccc cgtgatccgc tacgccaaga gcggcgacgt
    10321 ccgccgccgc ctcatgttcg aggaccagaa ccgcgggtat ccggcgaaca tcgcggtgct
    10381 cgactcgatg atcgccgacc gctaccaact cgcgaagatc ctcggctatc ccaactgggc
    10441 cgagtacatc accgagaaca agatgatcgg caacgcccag aacgcgtccg atttcatcca
    10501 gcgcctgaac gacctcacga tgaagcgggc gagcgacgag tacgcgatgt acctgaagcg
    10561 gaagcaggag gacgatccca cggcgaccgc ggtgaaccgc tgggaggttt cctactacgg
    10621 caggctgatc cggaagcgcg actacgattt cgacccgcag gcgatgcgcc cctacttcgc
    10681 gttcgccaac gtcgagaagg gcgtcctgga cgtgacgtcc aagatgttcg gcgtcaccta
    10741 caagaaggcg aacgtgccgg tgtgggatcc ttccgtcgag gcgtacgagg tctatgacgg
    10801 ctcgaagctc ctgggccgct tcttcttcga tctgcatccg cggcccggaa agtacaacca
    10861 cgccgcgaag ttcggcatcc gccagggagt ggccggcgtc caggtgccgg aggcagcgct
    10921 ggtctgcaac ttccccggcg ggacccccgg cgatccgggt ctcatggagc agtccgacgt
    10981 ggagaccttc ttccacgagt tcggccacct gatccatggc atcttcggcg gccagggacg
    11041 gtgggagccg gtgtccggca cggcgacgga gcgcgatttc gtcgaggccc cctcgcagct
    11101 gctggaggaa tggccgcagg acgcgaaggt tctccagacc ttcgcgaagc actaccagac
    11161 gggccagccg attccggccg acatggtcga gaagatgcgc cgcgccgacg ccttcgggcg
    11221 ggcgatgtcc tacgccaccc agaccttcta ctcggcggtc tcgctcaaca tctacaaccg
    11281 cccggcgtcc gaggtgaaca ccgatgcgat cgtcgcggcg gccgagccca agtacacgcc
    11341 cgtcccctcg atgccgggga cgcacatgca gtgctcgttc gggcatctcg acggctattc
    11401 ggcggtctac tacacgtacg agtggtcgct cgtgatcgcc aaggacatgt tcagcgcgtt
    11461 caacaagtcg aacctgctcg atcccacgat cgcccggcgc tatcgcgacg aggtgctgcg
    11521 ccccggcggc agccggcccg cgcgggagtc ggtgaaggcg ttcctgggcc gggactacga
    11581 tttcaaggcg ttcgacaagt ggctggcggg cgaggagacg gctcaggcgc cggcggccga
    11641 atcctcggct cccgcgagcg cgggacacta gaagggtgaa gagcccggcc gatcaggccg
    11701 ggggcagctc gacgacgatg ctgagaccgt gcgggtcgat cggttcggcc cgcacggttc
    11761 cttgatggcg ctccacgatc tccttcacga tggcgaggcc cagccccgaa cccttgccgt
    11821 cgctcccgtg cccggccgac tccgcgcggt agaaccgctc gaagacctgc cccatctcgc
    11881 cccgggggac gccgggcccg tcgtcctcca cccgaagcga atgcgtggca ccgaggcgcg
    11941 atccggacgg gtccacggag acccggaccg atccgccgcg cggcacgtag cggatcgcgt
    12001 tgttgagcag gttctccagc acctgctcca tgcggcgtcc gtccgcttcg atccacgccg
    12061 gaccggccgg cggctcccat tccaggcgca gcccggagtc gccgcagcgc ttctggtacc
    12121 gctccaccgt gtggccggcc agcgcggcga gatcgaggcg ctcgaggtgc agggccgaag
    12181 cgccggcttc cagccgggcc aggtcgaaga gatcgctgat cagccggtcg agccgcgccg
    12241 cctcctccag gaggccgcgc gtgtacttcg cgcgctcatc ggcggagacg cccacggccg
    12301 gatcgagcag cgtctcggcg aagccgcgga tcgacgtgag cggcgtcgcc agctcgtgcg
    12361 tgatgtcggc gaagagctgg cggcgctgct tctcgttccg ctccaggctg gcgcgcgcct
    12421 cggcgatacg ctcggtcatg cggtccagct gctcggcgat ccggccgatc tcgtcgccgc
    12481 ttctgtcctg gatccgcacc gacagatccc cctgggccac gcgcgtcgcg agcgcgtcga
    12541 tcgcgcgcag gcgccggacc agaagccgca cgatcaccag acccgcgagc cccgaggcga
    12601 gcacggcgaa ggggacgaag aggagcgccg tgcgcatctc ccagtagccc cagccggagc
    12661 gcggctgcca gcggcgcacc tgcaccgtac cgatcaccga ggcgccgcga cgcaccggcc
    12721 gctccgcgag gacgtcgaag cggggacggc cccgatcacg atcgcggccg cgcgcgcccc
    12781 cgaaggcgag ggagtcggag ccgggcgcgc ggaagcgggg cggggtgagc ggctccgagg
    12841 tgtacatgcc gtccgcgccg cggtagacga tcgagcttcc ccggaaatcc atgcggtccc
    12901 gcgcgctcgc cagcacgacg tggaccttgg cgtcggtcag cggttccggc agcgcggcga
    12961 tctcggtggc cgcgctcgcg gcgcccagct ccgcgcggga tcgcgcctcc cgggtctcga
    13021 gcggacggag gacgccgacc gcgacgaaga tctggagcag cgacccgccc acggtcgtga
    13081 ggaagaacat cccggcgatg gtccagaaga ggctgcgccg cggaccgggg gccgcggtca
    13141 tgggcgctgc gccggcgctg ccgccgcttt cgggcgcggt cacgacgcgg gctcggcgat
    13201 ccggtagccc gcgccccaga cggtctggag gtagcgcggg ttcgacgcgt ccgcctcgag
    13261 ctttcggcgg agccggctca ccaggctgtc gatcgaccgg tcgtccacga cacgatcggt
    13321 tccccacacc tgctccatca acgcctcgcg gctgaacacg cgcccggggc gcgacgccat
    13381 caggtagagg agatcgaatt cgagggtcgt gagcggcacg gccacgccgt cgcgctcgac
    13441 ggtgcgccgc gccgggtcga tcttgaggcc gccgacttcc aggagccggt cccccaccgc
    13501 gccgctggag cgccggagca gcacccgcac ccgcgccacc agctcggccg tgctgaaggg
    13561 cttggtcacg tagtcgtccg cgccgatctc gaagccgagg agcttgtcgc tctctcccga
    13621 gcgcgcggtg accatgagga tcggcgtgtc gctctccttc cggatgcggc gcgccacctc
    13681 gaggccgtcg agacccgcga gccccaggtc cagaaccacc agcgccggct tctgacgcgc
    13741 gaacgctgcg accgcgtgcc caccgtccgc cgacacctcg cactcgtagc ccgcggcctc
    13801 gaggttcttc acgatgaggc tcgcgatccg cgaatcgtct tctacgatca ggatcttctg
    13861 gctcatgacc tctcccgccg caagtgaacc gcccctggga cgccccattc tacccggacc
    13921 cgcgtggcag aagtgtggcg ccccggtgat gagtcggtgg cttcccggcg gcgcgcctcc
    13981 ctgtcccaat ttgccagtcc gccgccatct ctctgccaca ttcgacaggt accttcttca
    14041 tgaacgcgta ggagatcggt cgagggcccg tgccgctcct cgcgatgcag gaattacaat
    14101 aaagctcgga ggaacgatgt cgctgccgca ccccggcgcc gcgtccgacg ccaccgacag
    14161 gagaaccgcg acatgagaac gctccgctgg ctcctgccgc tgctcctcgc cgccgcgccc
    14221 gcgggggcga tggcggagcc gcggccgatc acgctgcagg aggccgtccg gctcgcgcgc
    14281 gagaacgcgc ccgccgtggt ccaggcgcgt gggcaggaac gctccgccga cgccgccaag
    14341 cgttcggcgt acgcggcgtt tctgcccagc ttgagcctct cggccggcgc cacgcggcgc
    14401 taccccgcgg ggggaacccg cgtggatccc cagacgcagc aggtgatctc gtcgggagac
    14461 ccctggggct acagcaccgg gctgacggcg tccgtggacc tg
//
LOCUS       DASSLZ010000178         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_569074, whole genome shotgun sequence.
ACCESSION   DASSLZ010000178 DASSLZ010000000
VERSION     DASSLZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569074"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VFM00_06990"
     CDS             complement(<1..1169)
                     /locus_tag="VFM00_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498029.1"
                     /translation="MFPDALRRCLLCLVALVAVLGAAVPAHAARPTGSTNPSGVPDSI
                     LERPVTLRRGIGNSHEPVTTTSPEAQAFYDQGLDYLESYVWVEAARSFHQALRLDPRL
                     AMAHVGLSRAYSGLEDAPAAERELAAARAAQSGASPRERRRIGIRAKQLDAMDDPKND
                     GNFRAYRKALDEALASDPDDIGLLLLRGNAEEPSPAGRGQLGTEASIAYYRHVLRLIP
                     DHASAHHFLTHTFEGMGMADSALVHGERYARLAPAIPHAAHMWGHALRRAGRIAEAVA
                     QFRRADAMERAYYESQAIEPRYDWHHSHNLNLMAAVAQDEGRRTVPPRARPDAPGDFV
                     PELLARESLELPSDLILAGRYAEALRLARAMTRDPSSATRVAGHVGAARALRGFRR"
     gene            complement(1290..2411)
                     /locus_tag="VFM00_06995"
     CDS             complement(1290..2411)
                     /locus_tag="VFM00_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HET7498030.1"
                     /translation="MASDDSGKAAGPGGAPAPRVVKGDQKFDLDRETFRARILARAYD
                     PAFEKIPGELERVIEVAWDAYHEYRKSPRTQPAGTEFADPTFPLAVEWLDARRRILEA
                     KKRHDDPHAPSRILVINASARTDQSCPGEISKTYRLTKIAREAMAGEPGVEVDFLDLS
                     RLTGEAHLVIYPCKACVSTAQPLCHWPCSCYPNHALGQVNDWMADIYPKWIAAHGVMI
                     VTPVNWYQSPSVLKLMIDRLVCADGGNPDPTTTRGKDAAMAKAIELAGWPYPKHLAGR
                     AFSVVVHADAAGAENLRRILVDWMTDLEMIPAGPVAGIDRYVGYYEPYATSHDDLDRD
                     AAFQEETRHAAKELVSMVRQIRSGQYRRPDEGLEEPRKK"
BASE COUNT          344 a          848 c          889 g          392 t
ORIGIN      
        1 cgccggaagc cgcgtagagc gcgagcggcg ccgacgtgtc ccgcgaccct ggtggccgag
       61 gatggatcgc gcgtcatcgc gcgcgccagc cggagcgctt ccgcgtacct gccggcaagg
      121 atcaggtcgc tgggcagctc cagcgattcg cgcgcgagaa gctccgggac gaaatcgccc
      181 ggcgcgtcgg gccgggctcg cggcggcacc gttcgacgcc cctcgtcctg ggccacggcg
      241 gccatcagat tcaggttgtg gctgtggtgc cagtcgtagc ggggctcgat cgcctggctc
      301 tcgtaatagg cgcgctccat ggcgtcggcc ctccggaact gcgcaacggc ttcggcaatc
      361 cgtcccgcgc ggcggagcgc gtgcccccac atgtgcgcgg cgtgcggtat cgccggcgcg
      421 aggcgcgcgt acctctcgcc gtgaaccagc gcgctgtcgg ccatccccat cccctcgaag
      481 gtgtgcgtca ggaaatggtg ggccgacgca tggtcgggga tcagccgcag cacgtgccgg
      541 tagtaggcga tcgaggcctc ggtgcccagc tggccccgcc ccgcggggct cggctcctcg
      601 gcgttgccac gcaagaggag aaggccgatg tcgtcgggat cggacgcgag cgcttcatcc
      661 agggcctttc tgtaggcgcg gaagttccca tcgttcttgg ggtcgtccat ggcgtcgagc
      721 tgcttcgcgc ggatcccgat gcgccgccgc tcgcgcgggc tcgcgcccga ctgcgccgcg
      781 cgggcggccg ccagctcccg ctccgcggcc ggggcgtctt ccagccccga gtaggcgcgg
      841 ctcaggccga cgtgcgccat ggcgaggcga ggatcgagcc ggagcgcctg atggaacgat
      901 cgcgcggctt ccacccagac gtaggactcc aggtagtcca ggccctggtc gtagaaggcc
      961 tgcgcctcgg gcgacgtcgt ggtgaccggc tcgtgcgagt tgccgatgcc gcggcgcagg
     1021 gtgaccggac gctcgagaat ggaatccggg acgcctgacg ggttggtcga gcccgtcggc
     1081 ctagcggcat gggccggcac cgcggcgccg agcacggcga ccagggccac gaggcagaga
     1141 aggcatcggc ggagcgcatc cgggaacatg aggaaccacc gggtggaaaa ttaggaacgg
     1201 cgcgcgggcg agaagcaccc aacctctctt ggatggattg gcgcgcgggc gcgattcagc
     1261 gattcggcgg cgctaaaccg atgccgggac tacttctttc gaggttcttc caggccctcg
     1321 tccgggcggc ggtactgccc gctccgaatc tggcgcacca tcgagaccaa ctccttcgcg
     1381 gcgtgccgcg tctcttcctg gaacgccgcg tcccggtcca ggtcgtcgtg gctggtcgcg
     1441 tagggctcgt agtagccgac gtagcggtcg atcccggcga ccggcccggc cgggatcatc
     1501 tcgaggtcgg tcatccagtc cacgaggatc cggcgcaggt tctccgcgcc ggccgcgtcc
     1561 gcgtggacga ccaccgagaa ggcgcggccc gcgaggtgct tcgggtaggg ccatccggcc
     1621 agctcgatcg ctttcgccat ggcggcgtcc ttcccgcgcg tcgtcgtcgg atcgggattc
     1681 cctccgtcgg cgcagaccag gcggtcgatc atcagcttga gcacgctcgg cgactggtac
     1741 cagttcacgg gcgtcacgat catcacgccg tgcgcggcga tccacttggg atagatgtcg
     1801 gccatccagt cgttcacctg accgagcgcg tggttcgggt agcaggagca cggccagtgg
     1861 cagagcggct gcgccgtcga gacgcacgcc ttgcagggat agatcacgag atgcgcttcg
     1921 ccggtgaggc ggctgagatc caggaagtcc acctccacgc ccggctcccc ggccatcgcc
     1981 tcgcgggcga tcttggtcag gcgataggtc ttcgagatct cgccgggaca gctctgatcg
     2041 gtgcgcgccg aggcgttgat cacgaggatg cgcgacggcg catgcgggtc gtcgtgccgc
     2101 ttcttcgcct cgaggatgcg ccggcgcgcg tccagccact ccaccgcgag cgggaaggtg
     2161 gggtcggcga actccgtgcc ggccggttgg gtgcgcgggc tcttccggta ttcgtgataa
     2221 gcgtcccacg ccacctcgat cacgcgctcc agctcgcccg gtatcttctc gaacgcgggg
     2281 tcgtaggcgc gcgccaggat gcgcgcccgg aaggtttcgc gatccagatc gaacttctgg
     2341 tcgcccttca cgaccctcgg cgccggggcg cctccggggc cggccgcctt gccgctgtcg
     2401 tcgctcgcca tggaatcagc cctcctgagg tgtcgggaac gtcgaggccg ggacgcgctc
     2461 cgcctcggaa ccg
//
LOCUS       DASSLZ010000179         7815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1584231, whole genome shotgun sequence.
ACCESSION   DASSLZ010000179 DASSLZ010000000
VERSION     DASSLZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584231"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..2241
                     /locus_tag="VFM00_07000"
     CDS             178..2241
                     /locus_tag="VFM00_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7498031.1"
                     /translation="MPTQSASTRVVDRWGDGPAREADDLDQLVYLSNLVGQEPRLVQP
                     GGGNTSIKVGDALLVKGSGTDLRTVGREGFTRLSLSALNALHMAETMLDADMMRFMVR
                     AMLAEGPTPSVETPLHSLLPARVIAHTHDVATMSLTNVSDAMAEQLVMELFAGAIVYV
                     PYVRPGFPLAQAVGGIVDRLPAGAIGLALAHHGIVVWGDEPRACYARLIGAVNAIEDY
                     LSAKRRSRPVPNAAPAPIPDAGVRRRSAELVLPVVRGVLGSANRVILHFDDADDVLAA
                     LADPRLPALARRGVATPEHILRAGAYPAWLDLDLSAPAESMAESVRAQLVRQRQDYEG
                     YHARHAAAGEPPLDDWAKVVLAPGLGMITAFRDKRGAVTANLCYRAVLETIANAESID
                     AFQFLSEADVFEFEHWPLERRKVDEQDAKERASLLLPRHVAIVIGAGSGIGKAAALRF
                     AKEGAHVVAADLDRSAAEAVAKEIGPGRAIGVELDVRDDESLARLIRQAVFEFGGLDS
                     LFYTAGRAPRFADITKLRREDLQRQMEVHYMGAVLAIGGAASVMRLQRLGGSIVASVS
                     KAALAPGRDATAYGGSKAALLQALRVAAVELGGDGIRVNAINADQIETPLFLEFVSQR
                     AAARGVSVEDQLETYRRRNVLGVSLIPAENVAGLAALLASEQFRYTTGDILTVDGGLP
                     EAFPR"
     gene            complement(2249..2623)
                     /locus_tag="VFM00_07005"
     CDS             complement(2249..2623)
                     /locus_tag="VFM00_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498032.1"
                     /translation="MAKLEMSAHMTVRPGCLEGFKRQAAELVRITREKDTNTLRYDWF
                     ISLDGTKCEVREMYTGPEGLIEHNRNILEARTKLFQDYADDHFMTVYGEVSPPLLELL
                     KTHQVGHTWFTFVQGIESKVGV"
     gene            complement(2774..3667)
                     /locus_tag="VFM00_07010"
     CDS             complement(2774..3667)
                     /locus_tag="VFM00_07010"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase/choline
                     kinase family protein"
                     /protein_id="HET7498033.1"
                     /translation="MPTNVQEDDSAVAVDAIRRELSLGTGIVRYSLGSVPVFAVGDGY
                     VVKLFPQAQRSYFEIERAVLPHIENLLPIPTPGLIAAGEHGAWLYLVMTRLSGRCLAE
                     AWPEIETGERAALVREVGEALAALHAIPAEEVASLAIDWPEFMAAQRASCRDRQLATH
                     LTSPWVDAADGFLARWMPEDDGSRVLLHTEVMREHLLVDRREGAWHIGGLLDFEDALR
                     GAREYEWACAGIFLTCGEPGLLGALLDGYGVRPDDELPMRCMAYALLHRYSNLRWYLE
                     RLPARGTEGDLESLARLWFAL"
     gene            3755..4357
                     /locus_tag="VFM00_07015"
     CDS             3755..4357
                     /locus_tag="VFM00_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498034.1"
                     /translation="MVTDRRPSRGKLRQEYDESSRLTAIEVPRSDGLAPLFGLLESAR
                     DAEARAPMSQACEELLRGFAEFYKIPPPSVKLLGVRPHRTREGRLAYELLGDYDLALT
                     RVRLWTRTPMIKKWTSSRTILSTLCHEFMHHLDVVQLGFPKSYHTTGFFERTHRLYLG
                     VTGQAYYPLAWQAPQRDGSRSIDWPATNRRKAAADRADRG"
     gene            complement(4368..5078)
                     /locus_tag="VFM00_07020"
     CDS             complement(4368..5078)
                     /locus_tag="VFM00_07020"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET7498035.1"
                     /translation="MVTRNATARRAGVLYFLFMIVAIVGEFLMPAFTVPGDAAATARN
                     ITTSEFMYRIDILLGFGTLVLFLFLVVNLYQLLRDADRGQAMLMVVLVSVGVAVALAN
                     TLLRLAPLVLLNGTDYLSAFTGPQREALALGFLRLHGAGAAISMGFWGLWLFPFGILV
                     LRSGFLPRVLGVLLMIAGSAYLVSSVTTLAMPAYKAAVTRVMMPLYFGEVPIIFWLML
                     KGAKVPQAALGATGAHPC"
     gene            complement(5080..5793)
                     /locus_tag="VFM00_07025"
     CDS             complement(5080..5793)
                     /locus_tag="VFM00_07025"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET7498036.1"
                     /translation="MTRTMNARIAGFTFLFYIGAGILSMVLSGWATHGEGTAARLASM
                     AQHPATVGVVVVLVLLQGFSALVLAATLYAITREEDADLAMLGLICRVVEGVLAAVVV
                     SRTLGLLWLSTAAGTQGLDTGPAQALAGFLFQAGTWSPVLGGAFFAVGSAVFSYLLLR
                     GRMIPVPLAVLGVVASALLVVGLPLRLAGFLHGPVTWFMWLPMAAFEVPLAFWLLVKG
                     VRVSSVHAAPPHGPQFTEA"
     gene            complement(5947..6780)
                     /locus_tag="VFM00_07030"
     CDS             complement(5947..6780)
                     /locus_tag="VFM00_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET7498037.1"
                     /translation="MRRAGSPRARGVTLKRAGPTALIADDEPLLREALARQLARAWPE
                     LEVVAQARNGREVVAQFEALRPDVCFLDVHMPGLSGVEAARQIGRRAHLVFVTAYDRY
                     AVEAFAQGALDYLVKPVEAGRLAETVARIKERLRAAEPAGNTEALLQQLAEEMARRRG
                     GAAPEPLRWIRAQAGEVLRLIPVEDVDYLRSDTKYTRVAWRGDAGRPAEALVRVPLKQ
                     LAAQLDPAQFAQVHRSVVVNLKSIDHVVRGDNESAGIHLKGRKEVLPVSRSYLHLFRQ
                     M"
     gene            complement(6738..>7815)
                     /locus_tag="VFM00_07035"
     CDS             complement(6738..>7815)
                     /locus_tag="VFM00_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7498038.1"
                     /translation="TMGAAPVARVDYSHLMHAPALALKPRGRALVFGWRRVRFTLLLS
                     LLLGVLFSFRWGPLGIVRISVLGLFGLLAFGLLEQWPKRLPSRLARWVLQVIGVALAM
                     PIGTLLFPVFGVEPSWEVQGLGLLVAPWAALAALVRQKDALARHQALAFDLERSELER
                     EALDARLRLLQAQVAPHFLFNTLANVQALVDAGSPRASEVLQSLIAYLRAAVPRLDEP
                     AATVDQELQLVRAYLELMHMRMPDRLQFSLHVDETALALQCPPMTLLTLVENAVRHGI
                     DPSEEGGRIDIHVHRRDNRCVVRVSDTGMGLQQGGNGLGTGLSTLRERLRLAFGGDAE
                     LRVSAQRPRGVCAELDLPAREASP"
BASE COUNT         1314 a         2705 c         2655 g         1141 t
ORIGIN      
        1 ccccccgacc ccagggagga aatgatctcg taggagccca gtcgggttcc ggaggcgagg
       61 cgcatgctgt cgaatctatc ccggaatcgc ccgccgcgcc atgtggacat cgggccgggg
      121 cccgccacct cacatgttgt cccgtctcac gacgcgagtg gggtagactc ctccgccatg
      181 cctacccaga gcgcatccac ccgggtcgtc gaccgctggg gcgacggtcc cgcccgcgag
      241 gcggacgacc ttgaccagct cgtctacctc tcgaatctcg tcggacagga accgcgcctc
      301 gtacagcccg ggggcggcaa cacctcgatc aaggtcggag atgctctgct cgtcaaggga
      361 agcggcaccg atctgcgcac ggtcgggcgg gaggggttca cacgcctttc tctctccgcc
      421 ctgaacgcgc tccacatggc cgaaacgatg ctggatgccg acatgatgcg attcatggtg
      481 cgcgcgatgc tggcggaagg ccccacgccc agcgtcgaga cgccgctcca ctcgctcctt
      541 cccgcccggg tcatcgcgca cacgcacgac gtcgccacga tgagcctgac caacgtgagc
      601 gacgccatgg cggaacaact cgtcatggag ctgttcgccg gagcgatcgt gtacgtcccg
      661 tacgtgcggc cgggcttccc gctggctcag gcggtgggcg gcatcgtgga ccggctgccg
      721 gcgggcgcca tcgggctggc cctggcgcat cacggcatcg tggtgtgggg ggatgagccg
      781 cgggcgtgct acgcgcgatt gatcggcgcc gtgaacgcga tcgaggatta tctgtccgcc
      841 aagcggcgga gtcgcccggt accgaatgcc gcgccggccc cgatcccgga tgccggggtt
      901 cgcaggcgct cggccgagct cgtgctcccc gtagtccgcg gcgtcctcgg gtcggcgaat
      961 cgcgtgatcc tgcacttcga cgacgccgac gacgtcctgg ccgcgctcgc ggatcctcgt
     1021 ctgccggcgc tcgcccgtcg cggagtggcg acgcccgagc acatcctgcg cgccggtgcc
     1081 tatcccgcct ggctcgacct cgatctatcc gcgccggccg aatccatggc cgagtcggtc
     1141 cgcgcccagc tcgtgcggca gcgccaggac tacgagggct accacgcccg ccacgccgcc
     1201 gcgggcgagc ctccgctcga tgactgggcg aaggtcgtcc tcgctcccgg gctgggcatg
     1261 atcaccgcgt tccgggacaa gcggggcgcg gtcacggcca acctgtgcta ccgcgccgtg
     1321 ctggagacga tcgcgaacgc ggagtcgatc gacgcgttcc agttcctgtc cgaagcggat
     1381 gtgttcgagt tcgagcactg gccgctcgag cggcgcaagg tggacgagca ggacgccaag
     1441 gaacgcgcct ccctgctcct tccccgccac gtcgcgatcg tgatcggcgc cggcagcggc
     1501 atcggcaagg ccgccgccct gcgcttcgcc aaggaaggcg cgcacgtcgt cgcggccgac
     1561 ctcgaccgct cggccgcgga ggcggtcgcc aaggagatcg gaccgggccg cgccatcggt
     1621 gtcgagctgg acgttcggga cgacgagagc ctcgcgcgcc tgattcggca ggcggtgttc
     1681 gagttcggcg gtctcgattc cctgttctac acggccggac gggcgccccg cttcgcggac
     1741 atcacgaagc ttcgtcgcga agacctgcag cggcagatgg aggtccacta catgggcgcc
     1801 gttctggcca tcggcggcgc ggcgtcggtc atgcggctcc agcgcctggg tggctcgatc
     1861 gtcgcgtcgg tgtccaaggc ggcactggca ccggggcggg acgccaccgc ctatggcggg
     1921 agcaaggcgg cgttgctcca ggcgctgcgc gtggccgcgg tcgagctggg cggcgatggg
     1981 attcgcgtca acgccatcaa cgccgaccag atcgagacgc cgctcttcct ggagttcgtg
     2041 agccagcgcg cggccgctcg cggcgtgtcg gtcgaggatc agctggagac gtaccggcgg
     2101 cgcaacgtcc tgggcgtctc gctcataccc gccgagaacg tggcgggcct ggcggcattg
     2161 ctggcgagcg agcagttccg ctataccacc ggggatatcc tcaccgtgga cggcggcctc
     2221 cccgaggcgt tcccgcgctg acgtcgcgct acacgcctac ctttgactcg atcccctgaa
     2281 caaaggtgaa ccacgtgtgc ccgacctgat gcgtcttgag cagctcgagc agcggcgggg
     2341 atacctctcc gtacacggtc atgaagtggt cgtcggcgta gtcctgaaac agcttcgtcc
     2401 gggcctcgag gatattgcgg ttgtgctcga tcagcccttc cggacctgtg tacatctcac
     2461 gcacttcgca cttggtccca tcgagactga tgaaccaatc gtagcgcagc gtgttcgtgt
     2521 ctttctcccg ggtgatccgg accagctccg cagcttgcct cttgaacccc tcgaggcacc
     2581 cggggcgcac ggtcatgtgc gcgctcatct ccaacttcgc catggctcct cctctcgctt
     2641 cggtcgcgcg ggtagattcc ctgaggatta cacgctagcg gggtaaggcg gcccggacca
     2701 tgggactgag gtataccggg cggggcctca gcgctgtcgg ttggatttca tcgccaccac
     2761 ggtagcgcgg gccttacagc gcaaaccaga ggcgcgcgag cgactccaga tcgccctccg
     2821 tcccacgggc aggcaggcgc tccagatacc agcgcaggtt gctgtaccgg tggaggagcg
     2881 cgtaggccat gcagcgcatc gggagctcgt cgtcgggtcg tacgccatag ccgtcgagca
     2941 aggcgccaag aagtcccggc tcgccgcacg tcaggaagat cccggcgcag gcccattcgt
     3001 actcccgcgc gccgcgcagc gcatcctcga aatccaagag accgccgatg tgccaggcac
     3061 cctcccggcg atcgaccagg aggtgctccc gcatcacctc ggtgtggagc agaacgcgcg
     3121 atccatcgtc ctccggcatc catcgcgcca ggaagccatc cgcggcgtcc acccacgggg
     3181 acgtcaggtg cgtggccagc tgccggtcgc ggcacgaggc gcgttgtgcc gccatgaact
     3241 ccggccagtc gatcgccagg gacgcaactt cctcagccgg tatcgcgtgc agggccgcga
     3301 gcgcctcccc cacttcgcgg accagggccg ctcgctcgcc ggtttcgatc tccggccacg
     3361 cctcggccag gcagcgaccg gacagacgcg tcatgacgag gtacagccag gcgccgtgct
     3421 cgccggcggc gatcaggcct ggcgtcggga tggggagaag gttctcgata tgcgggagca
     3481 ccgcgcgttc gatttcgaag tacgatcgtt gtgcctgcgg gaacagcttc acgacgtatc
     3541 cgtctccgac ggcgaagacc gggacggaac ccaaggagta gcggacgatg ccggttccga
     3601 gcgagagctc gcgccggatg gcgtcgacgg cgactgcgct gtcgtcttcc tgaacgttcg
     3661 tcggcatcgg gcactccgca cgaaggcgtc gttccacaag cgagccgatc ataccctctt
     3721 ccgcccgcct cgccgccgtt ctagattgcg aatcatggtg accgatcgca gaccttcccg
     3781 cggaaagctg cgtcaggaat acgacgagag cagccgcctc acggccatcg aggttccccg
     3841 gtccgacggt ctggcgccgc tgtttggcct gctcgagtcc gctcgggatg ccgaggcgcg
     3901 tgcgccgatg agtcaggcgt gcgaggagtt gctgcgtggg ttcgcggagt tctacaagat
     3961 tccgccgccg agcgtgaagc tcctcggggt gcggccgcac cgcacccggg agggccgtct
     4021 ggcctacgag ctattggggg actacgatct cgcgctcaca cgagtccgcc tgtggacgcg
     4081 gacgccgatg atcaagaagt ggacgtcgag ccggacgatc ctgtccacgc tgtgccacga
     4141 gttcatgcat cacctggacg tcgtccagct gggatttccg aagagctacc acacgacggg
     4201 cttcttcgag cggacccatc gtctctacct cggggtcacg ggccaggcgt actacccgct
     4261 ggcgtggcaa gcgccgcagc gcgacggttc ccgctccatc gactggccgg cgaccaaccg
     4321 ccggaaggcc gctgccgacc gcgccgaccg cggttgatcc cagccggtca acacggatgc
     4381 gcgcccgtcg cgccgagcgc tgcctgcggc accttcgctc ccttcaacat cagccagaag
     4441 atgatcggca cctctccgaa gtagagcggc atcatgacgc gggtgaccgc cgccttgtag
     4501 gccggcatcg ccagcgtcgt gacactgctg acgaggtagg ccgaacccgc gatcatcaac
     4561 aggacgccca ggacccgcgg caggaagccg gatcgtagga ccaggatgcc gaacggaaag
     4621 agccagaggc cccagaagcc catggagatg gcggcgccgg cgccgtgaag ccgcagaaat
     4681 cccagggcga gcgcctcccg ctgcggtccc gtgaaggcgg acaggtagtc ggtcccgttc
     4741 aacagcacga ggggcgccaa cctcaagagc gtgttggcga gagcgacggc gacaccgacc
     4801 gacaccagca ccaccatcag catcgcctga ccccgatcgg cgtcccggag cagctggtag
     4861 aggttcacga ccaggaacag gaagaggacg agggtgccga agccgagcag gatgtcgatg
     4921 cggtacatga actcgctggt ggtgatgttg cgggccgtgg ccgcggcatc cccggggacc
     4981 gtgaaggccg gcatcaggaa ctcgccgacg atcgccacga tcatgaagag aaagtagagc
     5041 actcccgccc gacgtgctgt cgcattccgg gtaaccatgt catgcctccg tgaattgggg
     5101 tccatggggc ggcgccgcgt gaacggaaga aacgcgaacg cccttgacga gcagccagaa
     5161 ggcgagcggc acctcgaagg cggccatcgg cagccacatg aaccaggtga ccggcccgtg
     5221 gaggaatccc gcgagacgca ggggaagccc cacgacgagc agtgccgagg caaccacgcc
     5281 gagtacggcc agcgggacgg ggatcatgcg gccgcgcagg aggaggtacg aaaacacggc
     5341 gctgcccacg gcaaagaacg ctccgcccag aaccgggctc caggttccgg cctgaaacag
     5401 aaacccggcc agcgcctgtg ccggcccggt gtccaagccc tgggttcctg ccgccgtcga
     5461 aagccagagc agccccagcg ttcgcgacac gaccacggcc gcgagcaccc cttcgacgac
     5521 gcgacagatc agccccagca tcgcgaggtc cgcgtcctcc tcgcgcgtga tggcgtacag
     5581 ggtcgcggcg agcaccaggg ccgagaagcc ctgcagcagg acaaggacga cgacgacacc
     5641 cacggtcgcc ggatgctggg ccatgctcgc gagcctggcg gcggtaccct ccccgtgggt
     5701 ggcccagccg gacaggacca tgctcaggat cccggcgccg atgtagaaca gaaacgtgaa
     5761 gccggcgatc cgtgcgttca tcgtgcgagt catgggcgtg tctcccaagg gtatccacag
     5821 cgctgacgct acccgcggag tgtgcgcctg ggaaaaggtg cggcggagcc gtttgcgacg
     5881 aatggcggga ttcggggatc gaaacgaggc gggcgcggac gaatcgcaac cgctggccga
     5941 cggtgcttac atctgccgga acagatggag gtagctgcgg ctgaccggca ggacctcctt
     6001 gcgtcccttc aggtgaatcc cggcggattc gttgtcgccg cgcacgacgt ggtcgatgga
     6061 cttgagattg accacgaccg atcggtgcac ctgggcgaac tgcgcggggt ccagctgcgc
     6121 ggccagctgt ttgagcggga cgcggacgag cgcttccgcg ggacggcccg cgtcgccgcg
     6181 ccaggcgact cgcgtgtact tggtgtcgga tcgcaggtaa tcgacgtcct cgacggggat
     6241 caggcgcagg acctcgcccg cctgggcgcg aatccagcgc agcggctccg gagccgcgcc
     6301 accgcgccgt cgcgccatct cctccgcgag ctgctgcagc aacgcctcgg tgttccccgc
     6361 cggctcggcc gcccgaaggc gttccttaat ccgcgccacg gtctccgcaa ggcgccccgc
     6421 ctccaccggc ttcaccaggt aatccaaggc tccctgggcg aaggcttcca ccgcgtagcg
     6481 gtcgtacgcc gtgacgaaca cgagatgggc gcggcggccg atctggcgtg ccgcctcgac
     6541 gcccgacagg cccggcatgt gcacgtcgag aaagcagacg tcgggtcgca gggcttcgaa
     6601 ttgcgcgacg acctcgcggc cgttgcgcgc ctgcgcgacg acctccaatt cgggccaggc
     6661 ccgcgccagc tgtcgcgcca gcgcctcgcg cagcagcggc tcgtcgtcgg cgatcagcgc
     6721 cgtcggtccc gcgcgcttca gggtgacgcc tcgcgcgcgg ggagatccag ctcggcgcag
     6781 acgccccggg gccgctgcgc gctcacgcga agctcggcgt cgccgccaaa ggccagccga
     6841 aggcgctcgc gcagggtcga gagtcctgtc cccaagccat tcccaccctg ctgcagaccc
     6901 ataccggtgt cgctgacgcg aaccacgcat cggttgtcgc gccggtgcac gtggatgtcg
     6961 atgcggcccc cctcttcgct gggatcgatg ccgtggcgca cggcgttctc caccagcgtc
     7021 agcagcgtca tcggcggaca ctgcagcgcc agcgcggtct cgtcgacgtg cagcgagaac
     7081 tgcaggcggt cgggcatccg catatgcatc agctcgagat aggcccggac cagctgcagc
     7141 tcctgatcga cggtggcggc cggctcgtcc aggcgcggca cggccgcgcg caggtacgcg
     7201 atcaggctct gcagcacctc ggaggcccgc ggcgacccgg cgtcgaccag tgcctgcacg
     7261 ttcgccagtg tattgaacag gaagtgtgga gcgacctgcg cctgcaaaag ccgcaggcgc
     7321 gcgtccagcg cctcgcgctc caattcgctc cgttccagat cgaaagccag ggcctgatga
     7381 cgcgccagcg cgtccttctg acgcaccagc gccgccagcg cggcccaggg cgcgaccagc
     7441 aggccgagcc cctgcacctc ccaggacggc tcgacgccga agacgggaaa gaggagcgtg
     7501 ccgatcggca tggcgagcgc gacgccgatg acttgcagga cccagcgcgc gagccgcgat
     7561 ggaagccgct tcggccactg ctcgagcagg ccgaacgcga gcagcccgaa gaggcccaga
     7621 accgagatgc gcacgatgcc aaggggtccc cagcggaagc tgaagaggac cccgaggagc
     7681 agcgagagga gcagcgtgaa ccgcacgcgt cgccagccga acaccagcgc gcgtcctcgg
     7741 ggtttcaggg ccagggcggg ggcgtgcatg aggtgagaat agtccacccg ggcgacgggc
     7801 gccgccccca tggtt
//
LOCUS       DASSLZ010000180        12019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1065685, whole genome shotgun sequence.
ACCESSION   DASSLZ010000180 DASSLZ010000000
VERSION     DASSLZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12019
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065685"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..2300
                     /locus_tag="VFM00_07040"
     CDS             318..2300
                     /locus_tag="VFM00_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7498039.1"
                     /translation="MDPVSKKRKISSPPRRRPTVPADSRYGPWWAWLTGILVLTFAVY
                     LPSLDNGFTNWDDAKYVTENLTLAHPSLHDILVRPLEGNYHPLTVLSLAWNYRLSQLH
                     PASYHGLNLLLHLANTALVFFFIRKLAGGLWAPVVTSLFFGIHPMHVESVAWIAERKD
                     VLFVFFYLAGLIAYLEYRDSKRGGWLAATLAACVLSLASKPAAVTFPVALMLVDAYRG
                     RRFDTAAIVEKTPFFALAIVAGLLTLHAQKAVGAIVDTPVFPLFSRVLFAAYGTVSYV
                     VKLLVPLGLSAIYPYPVGGRVGPEFYVAFAALVVLLPAAVYLFRHNRAVLFGLAFFLL
                     NIALVLQFVTVGHALMADRYTYLPYVGLFFALSYWMDAGPPPWAPGSSVKTLTAGLLL
                     LLVPVCAVQTWKRCGVWKDSATLWTDTIRHYPNRIYDAYFLRGTYYHHAVGDPAAALA
                     DYDEALKLNPRAAFALANKGTLLFDLGRADSAYVYLDRALQLKPDLAGALNNRGAIKG
                     QRGDLPGAVADFSRAIASEPLFRDPYENRAVGYYMAGQFDKAIADTRRALELDPANPE
                     NHALWDAIGQYEQKLHRYQEAVTAHDEAIRRAPYGDPRIPGYYLNRSFAYEGLGNREA
                     AIRDARQAEQLGATLAPGYLKKLGIAAAPAAPPAAP"
     gene            2373..3239
                     /locus_tag="VFM00_07045"
     CDS             2373..3239
                     /locus_tag="VFM00_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET7498040.1"
                     /translation="MKLVACPQCHAQYDAGLATGPTIPCRCGATFPSEPPRAKDAAVT
                     RCAACGALVGERERVCSYCGAEVVRRPAGSGPVCPECYAQNPESARYCVACGVALLPQ
                     PVRAAAGALPCPVCTGSTLAARNLGGIWVEECPSCLGLWTPGDVIDRLIDRIRERLAQ
                     DGASVMARHERRSKWRPEFTYRKCPECGVMMQRRNFGRRSGVVLDWCASHGTWLDAHE
                     LEDVASFVIDGGLRGADPAQGDVSLAADPKRVAAVAAAETILAKERMKAERRERPISL
                     GDLFTLLLRQRL"
     gene            3288..4376
                     /locus_tag="VFM00_07050"
     CDS             3288..4376
                     /locus_tag="VFM00_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7498041.1"
                     /translation="MGIVEKLRGELVDIIEWVDDSRNTLVWRFPRYHNQIKNGARLIV
                     RPGQSAVVVEQGRVADVFGPGTYELTMKNLPLLSTLRGWKYGFDSPFKAEVYFVATRQ
                     ITELKWGTPNPVILRDPSLGPLRVRAFGTYTLRAAKPEALLAELVGTDGSFQADEIDV
                     LLRSLIATAFADLIATTQIAIADLSASYLRLSEELRKRVLAKIDQEYGLDIPQLYIVN
                     VSVPDSVEQAMDARSGMEVIGDVERYQRYQVGAATPLAAANPSGGLAGAGVGLGMGLS
                     YAGAAASRPVNAPVIPPVPLADAVWHVATAGTASGPFTLEQIRQGMAEGKVTPQSHVW
                     TAGMSAWAPIASVPALAALLTPPPPPRG"
     gene            4461..4940
                     /locus_tag="VFM00_07055"
     CDS             4461..4940
                     /locus_tag="VFM00_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003192632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HET7498042.1"
                     /translation="MTDPLRFRVTPLPAEEFAPLFRLSDSELASRGMLRMVADSKPGF
                     PCRVSLEDAEVGESVILLPYVHHRTESPYRASGPIFVREGAATAAPAVGEVPEAVRTR
                     LLSVRAYDAEGMMVRSEVAEGSALADCARRHFADPSVATLHVHNAKAGCYSCRIERA"
     gene            complement(4945..7347)
                     /locus_tag="VFM00_07060"
     CDS             complement(4945..7347)
                     /locus_tag="VFM00_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7498043.1"
                     /translation="MPIATGTRLGSYEILSPLGAGGMGEVYRARDTRLGRAVAVKVLP
                     EAVASSADRLARFEREARTVAGLNHPNIVTLYSVEDERGVRFLTMELVEGRTLDHLIA
                     PGGLPLARVLDLAIPLTSALAAAHERGVIHRDLKPGNVMVTHDGWVKVLDFGLAKMSQ
                     GDLGGEGATIGATAESPISGHGQVLGTVPYMAPEQVRAEAVDARSDLFSLGIILYELA
                     TGRRPFTGPTPADVSSAILRDVPVPVLSLRGDVPRDLDRIITRCLEKNARDRIQTARD
                     VYNELRYVQREAERALGLAAPGSAPAGPATPPPLPSPPPAPPSSRSFTTPSSSVSAPP
                     RDAPSIAVLPFVNRSRGEEDEYFSDGLADELLNVLTKVRGLRVAARASSFQFKGKNED
                     LAVIGQKLNVATLLDGSVRKAGNRVRISVQLIHASDRVQLWSETYDRSLDDIFAVQDD
                     IAQSVVKELRTTLLGEEPDSKASGLAQADVAAAAKGHGDNPEAHRLYLQGKYLIDRMN
                     REDNSKAIEYLNEALSKDPKHALAWTELSRAHALSGGYGWEPVNDAYRKAREAASKAL
                     ELAPDLPEAHVRLSAIQRMYDWDFRAAEESSRRALQLAPGSPAALGAAGALAGILGRL
                     DAAEAMHLRALQQDPLSAASYSSLAFVYRNMGRLPDAERYLRKALELSPNRVGGRHGL
                     GIVLAEMGRFEEALAEAQREGAEWARLTAIAYVHQLAGRKAESDEALRQLEERHAVDS
                     PYQVAAIHAVQEDVDGTFAWLERAYQERDTGMASIGSEPLFRRLHGDPRWKPLMKKIG
                     LA"
     gene            complement(7418..8314)
                     /locus_tag="VFM00_07065"
     CDS             complement(7418..8314)
                     /locus_tag="VFM00_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7498044.1"
                     /translation="MTLTGLHLLLTYGCTYECDHCFVWSSPEQPGTITIPQVDEILRQ
                     TRALGTVEWFYFEGGEPFLYHGLLRWSALRARKLGFRVGIVSNAYWATSEEDATEWLR
                     DLSGVVDDLSLSCDAFHGGADQERRVDRARRAALRLGMPVDTIQVAPVEDSAAGRVGQ
                     LPAGESAVMFRGRAAAVLAPHAPHVPWASFDRCPHEDLREPGRVHVDPLGWVHVCQGI
                     AIGNLFRTPLVEIVARYDPEAHPITGPLLAGGPAELARRHGVGHGEAYADACHLCDRT
                     RAALRVRFPEELGPAQMYGPVR"
     gene            complement(8311..10893)
                     /locus_tag="VFM00_07070"
     CDS             complement(8311..10893)
                     /locus_tag="VFM00_07070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7498045.1"
                     /translation="MTERWAQVGELFEAALRQPREQRAAFVKESCAGDRELETEVRSL
                     LASHDAAGGFLEPEGGGISLPFPEGPAPGQSIGSWRVVRPLATGGMGVVYLVEREDGQ
                     FHQRGALKLIRHGLATQEMVQRFLRERQILATLDHPNMARLLDGGTTPEGLPWLVMEY
                     VEGVPLYEWCSEKAPTLRERLRLFLALCGSVEAAHRRLVLHRDIKPANVLVTAEGTPR
                     LLDFGVAKIFSAEGAPVSELTTARAPLTPEYASPEQLRGGDITTASDVYSLGVLLFEL
                     ATGARPYPTRAEGATQLLRTVLEKDPLRPSTAAATRLGTAAERTRSLPSPPTGGPGAL
                     SRALAGDLDNIILKSLAKEPERRYGSVEELGADLRRYLDGRPVEARPSTWRYRTSKFV
                     RRNRVAVGMATVAALAIVAGAAFSLWSAREARRERAVAERRLRDVATMANTVLWDVNE
                     GLASMPGTTPLREKIVDSATKYLNGIAAEGVQDTALIRTLADGFDKLGTVQGYVWSAN
                     VGRSEEGYRSLKKAYELRDRLVRVNPAQEEYKLDLASSATKLCNFDREHGRAVEATAM
                     SRRALELMTELRTMRPDVRRYRINIPRLLHNDGLTLIEAGKIDEGVAQIRRGVDSFAA
                     LVVWEPKEPSHRRTLAQASTGLAEALVLQPGGSDSALAVLGRARTLLDDLIRENPDDA
                     DLLRRRAAVHYNTGRIYLLNGNDPVSALGEAEACAAATRSAAQKDPGNDDAVVSDLVA
                     RTFLGHVHAAAGHAALAEDLLGGVIPDLVRRAMTDTTDTRFAQELIEAKLAMGLVELD
                     RAKKGAGAASAAHRQAARQWLVGAREARDRLAEQTGPWSFSREETAAIETSLAACEQG
                     PVTP"
     gene            complement(10890..11492)
                     /locus_tag="VFM00_07075"
     CDS             complement(10890..11492)
                     /locus_tag="VFM00_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7498046.1"
                     /translation="MDVPESQGAPSPSEVTQTFLRWRQGDASALHALLPLVYEEMRRL
                     AGAYLRDESAGHTLQPTALAHEAYLRLLDQRHVSWQNRAHFMGLAAQAMRRILIDHAR
                     RRGAQKRGGDAVHVTLEDSDAIAAGDEPLGVAAEDLNDALDRLAALDERQARVVELRF
                     FTGLSVEETAEVLGVSAATVKRDWTLARAWLHRELKGRIA"
     gene            complement(11540..>12019)
                     /locus_tag="VFM00_07080"
     CDS             complement(11540..>12019)
                     /locus_tag="VFM00_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HET7498047.1"
                     /translation="ATFPGETIEGHFGLAVSDYTHSTAPNRRYPDLITQRLLRAALEG
                     IAIPYAPDALRSLAGHCTEREDDVNKIERLLDKAAAACLLSSRLGQEFDGIVTGASDK
                     GTWVRLLDPAVEGRVEQGQAGLDVGDRVRVKLVHTDCERGFIDFARTGHAPVGRRTR"
BASE COUNT         1716 a         4266 c         4153 g         1884 t
ORIGIN      
        1 cgttgagcgc gaagtggaac gacgggccgt gcggcaggcc cagagcaccg gcgagcgccg
       61 gcgagatgcc cagctcggcc tcgtagaccg ccgctccagg gtcgtgggtc agaacgcccg
      121 tggcggggtc gtacgctccc ggcctacgcc gggggcaacg ttggtgtccg gcaatggctt
      181 ggcatcggcg cggcgggggg agcctgtcca gggccggccc ggccggctgc caccctgtca
      241 gtgtcatgac agagaggcag ctacgaccgg tatcgcgtga ttcctgagag gctctcgcag
      301 tatgatcgat gacttccatg gatccggtct cgaagaagcg caagatctcg tccccgccga
      361 ggcggcgacc caccgtgccg gcggactcgc gctacgggcc atggtgggcc tggctcaccg
      421 gcatcctggt gctcaccttc gccgtctacc tccccagtct cgacaatggc ttcacgaact
      481 gggatgacgc caagtacgtc accgaaaacc tgacgctcgc gcacccgtcc ctgcacgaca
      541 tcctggttcg acccctggaa gggaactacc acccgctgac cgtcctgtcg ctcgcgtgga
      601 actaccggct ctcgcagctg caccccgcgt cctatcacgg gctcaacctt cttctccacc
      661 tggcgaacac cgccctggtc ttcttcttca tccggaagct ggccggaggg ctctgggccc
      721 cggtggtgac gagcctcttc ttcggaatcc atccgatgca cgtcgaatcg gtggcctgga
      781 tcgccgagcg caaggacgtg ctcttcgtct tcttctacct ggccgggctg atcgcgtatc
      841 tcgaataccg ggattccaag cgcgggggat ggctcgcggc gacgctggcc gcgtgcgtgc
      901 tgtcgctcgc gtccaagccc gccgcggtca ccttcccggt ggcgctgatg ctggtcgacg
      961 cgtaccgcgg ccgccgcttc gacaccgccg cgatcgtcga gaaaaccccc ttcttcgccc
     1021 tggcgatcgt cgcggggctt ctcaccctgc acgcccagaa agccgtgggc gccatcgtcg
     1081 acacgcccgt gttcccgctc ttctcgagag tgctcttcgc ggcgtacgga accgtgtcgt
     1141 acgtggtgaa gcttctcgtg cccttgggtc tctcggcgat ctatccctac ccggtcggtg
     1201 gacgggtggg ccctgagttc tacgtcgcgt tcgccgcgct cgtcgtcctt ctgccggccg
     1261 cggtctacct gttccgccac aaccgggccg tcctgttcgg cctggcgttc ttcctgctga
     1321 acatcgccct cgtgctccag ttcgtcaccg tgggccacgc cctgatggcg gatcgctata
     1381 cctatctgcc ctacgtcggg ctcttcttcg ccctctccta ctggatggac gcgggccccc
     1441 cgccctgggc tccgggctct tccgtgaaga cgctcaccgc gggccttctc ctgctgctcg
     1501 ttccggtctg cgccgttcag acctggaagc gctgcggcgt ctggaaggat tcggccaccc
     1561 tctggaccga cacgatccgc cattatccga accggatcta cgacgcctac ttcctgcgcg
     1621 gtacctacta ccaccatgcg gtgggcgatc ccgccgccgc gctggccgac tacgacgaag
     1681 ccctgaaatt gaatccccgg gcggcgttcg ccctggccaa caaggggacg ctcctcttcg
     1741 acctcgggcg cgcggactcg gcgtacgtct acctggatcg cgcgctccag ttgaagcccg
     1801 acctggcggg cgcgctgaac aaccgcggcg ccatcaaggg gcagcgcggc gatctcccgg
     1861 gggcggtcgc cgatttttcg cgggcgatcg cgtcggagcc gctattccgg gatccgtacg
     1921 agaaccgggc ggtgggctac tacatggcgg ggcagttcga caaagcgatc gccgatacgc
     1981 gccgcgcgct ggagctggat ccggcgaacc cggagaacca cgcgctctgg gacgcgatcg
     2041 gccagtacga gcagaagctg caccgctacc aggaggccgt gaccgcgcac gacgaggcga
     2101 tccggcgcgc gccgtacggc gatccgcgca ttcccggcta ctacctgaac cggagctttg
     2161 cctacgaggg cctggggaat cgcgaggccg cgatccgcga cgcgcgccag gcggagcagc
     2221 tcggcgcgac cctggcgccg gggtatctga agaagctggg aatcgccgcg gccccggcgg
     2281 ccccgcccgc cgcgccctaa gtctcaagtt ccgcccgccg ccgccgatcc tttccgggac
     2341 ctttcgtccg taccggcccg gagcgcgccg ccttgaagct cgtcgcctgt ccccagtgcc
     2401 atgcccagta cgacgccggc ctcgcaacag gcccgacgat cccgtgccgc tgtggcgcga
     2461 cgtttccgtc ggagccgccg cgggccaagg acgccgcggt cacgcgctgc gcggcatgcg
     2521 gcgccctggt aggcgagagg gaacgggtct gctcctattg cggcgccgaa gtcgtccggc
     2581 gtccggcggg ctcggggccg gtctgtcccg agtgctacgc gcagaatccg gagtcggcgc
     2641 gctactgcgt cgcctgcggg gtggccctcc tcccgcagcc ggtgcgggcc gccgccggcg
     2701 cgctgccctg tccggtctgc acggggtcga cgctcgcggc gcgaaacctg ggcgggatct
     2761 gggtcgagga atgcccctcc tgcctgggcc tctggacgcc gggcgacgtg atcgaccggc
     2821 tgatcgaccg gatccgcgag cggctcgcac aggacggcgc ctccgtgatg gcgcgccacg
     2881 agcggcgctc gaagtggcgg cccgagttca cctatcggaa gtgcccggag tgcggcgtga
     2941 tgatgcagcg gcggaatttc ggccgccgct ccggcgtggt tctcgactgg tgcgccagcc
     3001 acggcacctg gctcgacgcc catgagctgg aggacgtcgc gtcgttcgtg atcgatgggg
     3061 ggcttcgcgg cgcggatccg gcgcagggcg acgtgtcgct cgccgcggat cccaagcgcg
     3121 tcgcggccgt cgccgccgcg gagacgatcc tcgcgaagga gcggatgaag gccgagcgcc
     3181 gggagcgtcc gatcagcctt ggagacctgt tcacgctgct cttgcgccag cgcctgtaga
     3241 acgccgggcg ccggcgtggc gccggcggcc atgaacgagg aggaacgatg gggatcgtgg
     3301 agaagctgcg cggcgagctg gtcgacatca tcgagtgggt cgacgactcg cggaacacgc
     3361 tggtctggag atttccgcgg taccacaacc agatcaagaa cggcgcccgc ctgatcgttc
     3421 ggcccggcca gtcggccgtc gtcgtcgagc aggggcgcgt cgccgacgtg ttcggccccg
     3481 gcacctacga gctcacgatg aagaatctcc cgctcctgag cacgctgcgc ggctggaagt
     3541 acggtttcga cagccccttc aaggcggagg tctacttcgt cgccacgcgc cagatcacgg
     3601 agctcaagtg ggggaccccc aacccggtca tcctgcgcga tccatcgctc gggccgcttc
     3661 gcgtgcgggc cttcggcacc tacaccctcc gggcggccaa gcccgaggcg ctgctcgcgg
     3721 agctggtcgg caccgacggg tcgttccagg ccgacgagat cgacgtgctc ctccgctccc
     3781 tcatcgcgac cgccttcgcc gatctgatcg cgaccaccca gatcgcgatc gcggatctct
     3841 cggccagcta cctgcgcctc tccgaggagc tccggaagcg cgtgctcgcg aagatcgatc
     3901 aggaatacgg cctggatatc ccgcagctct acatcgtgaa cgtttccgtt cccgactcgg
     3961 tggagcaggc aatggacgcg cgttcgggca tggaggtgat cggcgacgtg gagcggtatc
     4021 agcgctacca ggtcggcgcc gccacgccgc tcgcggccgc gaacccttcg ggcgggctgg
     4081 ccggagcggg cgtgggactg ggaatgggcc tctcctacgc cggcgccgcg gcgtcacggc
     4141 ctgtgaacgc cccggtcatt ccccccgtgc cgctggccga cgccgtctgg cacgtcgcga
     4201 ccgccggaac ggcgtccggc cccttcacgc tcgagcagat ccgccaggga atggccgagg
     4261 gaaaggtgac gccgcagagc catgtctgga cggcggggat gagcgcgtgg gcgccgatcg
     4321 cgtcggtgcc ggcattggcc gcgctcttga cgccgccacc gccgccgcgg ggatgagttc
     4381 cgaagcaagc atcggatagc cgggtcggtc gccggacctc acgatcgtgg tcgacgttca
     4441 cccgatcgaa ggaggatccg atgaccgacc cgctgcgctt ccgcgtcacg ccgcttcccg
     4501 ccgaggagtt cgcgcccctc ttccgcttga gcgattccga gctcgcgtcc cgaggcatgc
     4561 tccggatggt cgccgattcg aaaccgggct ttccctgccg cgtgagcctg gaggatgccg
     4621 aggtggggga gagcgtgatc ctgctcccgt atgtgcatca tcgaacggag agtccctacc
     4681 gggcatcggg tcccatcttc gtccgggaag gggccgcgac ggcagcgccg gccgttgggg
     4741 aagtccccga agccgtgcga accaggcttc tatcggtgcg cgcctacgac gcggaaggga
     4801 tgatggtgcg gtcggaggtc gcggagggca gcgccctggc cgattgtgcg aggcgccact
     4861 tcgccgaccc gtcggtggcc accctccacg tccacaacgc gaaggcgggg tgctacagct
     4921 gccggatcga gcgggcctga tccttcacgc gaggccgatc ttcttcatga gcggtttcca
     4981 ccgggggtcc ccgtgcaggc gccggaacag gggctcgctt ccgatcgagg ccatcccggt
     5041 gtcccgctcc tgataggcac gctccagcca ggcgaacgtg ccgtccacgt cctcctgcac
     5101 cgcgtggatc gcggcgacct ggtaggggga gtccacggcg tgcctttcct cgagctgccg
     5161 gagcgcctcg tccgactccg ctttccggcc ggcgagctgg tgcacgtagg caatcgccgt
     5221 gagtcgggcc cactccgcgc cctctcgctg ggcctccgcc agcgcctcct cgaaccggcc
     5281 catttcggcc aagacgatgc ccagcccgtg gcggccgccg acgcgattcg gagaaagctc
     5341 caatgccttg cgcaggtagc gctcggcatc gggcaaccgc cccatgttgc ggtagacgaa
     5401 cgcaagggag ctgtacgagg cggcgctcag cgggtcctgc tgaagggctc gaaggtgcat
     5461 ggcctccgcc gcgtcgagcc gtccgagaat cccggccagg gcccctgccg cgccgagcgc
     5521 ggcgggactg ccgggagcaa gctgcagcgc gcgccggctc gattcttccg ccgcccggaa
     5581 atcccagtcg tacatccgct ggatggcgct gagccgcacg tgggcctcgg gtaggtcggg
     5641 tgccagctcc agggccttgc tcgccgcctc tcgggccttg cgataggcgt cgttgacggg
     5701 ctcccagcca tagccgccgc tcagcgcgtg ggctcgtgaa agctccgtcc aggccagcgc
     5761 atgcttgggg tccttcgaca gcgcttcatt caggtactcg atcgccttgc tgttgtcctc
     5821 ccggttcatc cgatcgatca ggtactttcc ttgaaggtag agacgatgcg cctcgggatt
     5881 gtccccgtgc cctttcgccg cggcggccac gtcggcctgg gccaggccgc tcgccttgga
     5941 atcgggctcc tcgccgagca gggtggtgcg cagctccttg acgaccgact gagcgatgtc
     6001 gtcctgcacc gcgaagatgt catcgagcga gcggtcgtag gtctccgacc agagctgcac
     6061 gcggtccgag gcgtggatca gctgcaccga gatccgcacc cggttgcccg ccttgcgcac
     6121 gctcccgtcg aggagcgtcg ccacgttcag cttctggccg atcaccgcga ggtcctcgtt
     6181 cttccccttg aactgaaagg acgaggcgcg cgcggccacg cgaagccccc gaaccttggt
     6241 gagcacgttg agcagctcgt cggcgagacc gtccgagaaa tactcgtcct cttcgccccg
     6301 gctccggttc acgaagggga gcacggcgat cgacggagca tcccgaggcg gcgccgacac
     6361 cgaagaggaa ggggtcgtga aggaccggga cgaaggcggc gccggcggcg gcgagggtag
     6421 cggcggcggg gtcgcgggac cggccggcgc gcttcccgga gccgccaggc ccagcgcgcg
     6481 ctcggcctcg cgctggacgt agcgaagctc gttgtacacg tcgcgcgcgg tctggatgcg
     6541 atcccgcgcg ttcttctcca ggcaccgcgt gatgatccgg tcgagatccc gaggcacgtc
     6601 gccccgcaac gagagcaccg ggaccggaac gtcgcggagg atggccgagc tcacgtccgc
     6661 cggcgtcggg ccggtgaacg gcctccgtcc cgtcgcgagc tcgtagagga tgatgccgag
     6721 cgagaagagg tccgaccggg cgtccacggc ctcggcgcgg acctgctcgg gcgccatgta
     6781 cgggacggtg ccgaggacct ggccgtggcc cgagatcggc gactcggccg tcgcgccgat
     6841 ggtggcgccc tcgccgccca ggtccccctg gctcatcttg gcgaggccga agtccagcac
     6901 cttgacccac ccgtcgtgcg tcaccatcac gttccccggc ttcaggtcgc ggtggatcac
     6961 gccgcgctcg tgcgcggcgg ccagcgcgct cgtgagcgga atcgcgaggt ccaggacgcg
     7021 cgcgagcgga agaccgcccg gggcgatcag gtggtccagg gtgcgtccct cgaccagctc
     7081 catggtgagg aagcggacgc cgcgctcgtc ttccacggag tagagcgtca cgatattcgg
     7141 atgattgagc cccgcgacgg ttcgggcctc gcgctcgaag cgcgcgaggc gatccgcgga
     7201 ggaggcgacc gcttcgggaa gtaccttcac ggcgaccgcg cgccccagcc gcgtgtcgcg
     7261 cgcgcggtag acctcgccca tgcccccggc gccgaggggc gaaaggatct cgtacgatcc
     7321 gaggcgggtt ccggtggcga tgggcatggc ggaagagtct agcccttcgc cgcgggattg
     7381 gaaacgggcg cagcgcgcca cggcacgacg gtcgccttca gcgcaccggg ccgtacatct
     7441 gggcggggcc cagctcctcg gggaagcgga cgcgaagcgc ggctcgcgtt cgatcgcaga
     7501 ggtggcaggc gtcggcgtac gcctcgccgt ggcccacccc atgccggcgc gccagctcgg
     7561 cgggtccgcc cgcgagcagc gggcccgtga tcggatgcgc ttcggggtca taccgcgcca
     7621 cgatctcgac aaggggcgtg cggaacaggt tcccgatcgc gattccctgg cagacgtgaa
     7681 cccagccgag cggatcgacg tggacgcgcc ctggctcacg gaggtcttcg tgcgggcagc
     7741 ggtcgaagga cgcccacggc acatggggcg cgtgcggggc gagaacggcc gccgcgcgtc
     7801 cgcgaaacat gacggccgat tcgccggccg gaagctgccc cacgcgaccc gccgcgctgt
     7861 cctccaccgg cgccacctgg atcgtgtcca cgggcatgcc gagccgcagg gcggcgcggc
     7921 gcgcgcggtc cacccggcgc tcctggtccg cgccgccatg gaacgcgtcg cagctcaggg
     7981 agaggtcatc gaccactccc gagagatccc gcagccactc ggtcgcatcc tcctcgctcg
     8041 tcgcccagta agcgttggat acgatcccca cgcggaatcc aagcttgcgg gcccggagtg
     8101 cgctccaacg gaggagcccg tggtagagaa agggctcgcc cccttcgaag tagaaccact
     8161 ccacggtgcc tagcgcgcgc gtctgacgaa ggatctcgtc tacctgcggg atcgtgatcg
     8221 ttcccggctg ttcggggctc gaccacacga agcagtggtc gcactcgtac gtgcagccgt
     8281 aggtgaggag caggtgaaga cctgtcagcg tcatggcgtc accggaccct gctcgcacgc
     8341 agcgagcgat gtttcgatcg ccgcggtctc ttcgcgggag aacgaccatg gccccgtctg
     8401 ctccgccagc cggtcgcggg cctcgcgcgc gccgaccagc cattgccgcg cggcctgccg
     8461 gtgcgccgcg gacgcggcgc ccgcgccctt cttcgcccgg tccagctcca ccagccccat
     8521 ggccagtttg gcctcgatca gctcctgggc gaaccgggtg tcggtggtgt cggtcatcgc
     8581 gcggcgcacc aggtcgggga tgacgccgcc gagcagatcc tcggcgagcg ccgcgtggcc
     8641 cgcggccgca tgcacgtgcc ccaggaaggt gcgggcgacc agatcgctga ccaccgcgtc
     8701 gtcgttgccg ggatccttct gcgccgccga gcgcgtggcc gcggcgcatg cttcggcctc
     8761 gccgagcgcg ctcacgggat cgttcccgtt caggaggtag atgcggccgg tgttgtagtg
     8821 gaccgccgcg cggcgccgca ggagatcggc gtcgtcggga ttttcgcgga tcagatcgtc
     8881 gaggagcgtc cgcgcgcgcc ccagtacggc cagcgcgctg tccgatccgc cgggctgcag
     8941 cacgagcgcc tcggccaatc cggtggaggc ctgcgccagc gtgcggcggt gcgacggttc
     9001 cttcggctcc cagaccacca gcgccgcgaa cgagtccacg ccgcgccgga tctgagccac
     9061 cccttcgtcg atcttccccg cctcgatcag cgtgaggccg tcgttgtgaa gcagccgcgg
     9121 gatgttgatg cgatagcgcc gcacgtccgg gcgcatcgtc cgaagctcgg tcatcagctc
     9181 cagcgcgcgg cgcgacatcg ccgtcgcctc gaccgcgcgt ccgtgctcgc gatcgaagtt
     9241 gcagagcttg gtcgccgagg acgccaggtc cagcttgtat tcttcctgcg ccggattgac
     9301 gcgcaccagc cggtcgcgca gctcgtaggc tttcttcagc gagcgatagc cctcttcgct
     9361 ccggccgacg ttggcgctcc agacataccc ctggacggtc cccagcttgt cgaacccgtc
     9421 ggccagggtg cgaatcaacg ccgtgtcctg gaccccctcg gcggcgatcc cgttcaggta
     9481 cttcgtcgcc gaatccacga tcttctcgcg gagcggggtg gtcccgggca tggaggccag
     9541 gccctcgttc acgtcccaga gcacggtgtt cgccatcgtc gccacgtcgc gcaggcggcg
     9601 ttccgcgacg gcgcgctcgc gccgcgcctc gcgggcgctc cagagcgaga aggcggcgcc
     9661 ggcgacgatc gccagcgcgg cgaccgtcgc catgccgacg gcgacccggt tccggcgcac
     9721 gaacttcgac gtgcgatacc gccacgtgga gggacgggcc tcgaccggcc gaccgtcgag
     9781 atagcgcctc aggtcggcgc ccaattcctc caccgacccg tagcgccgct cgggctcctt
     9841 cgcgagcgat ttcaggatga tgttgtcgag atctcccgcc agggcgcggc tcagggcgcc
     9901 gggaccgccg gtcggcgggg agggaaggct gcgcgtccgc tccgcggccg tgccgagccg
     9961 cgtcgccgcg gccgtgctgg gccgcagcgg gtccttctcg agaacggtcc ggaggagctg
    10021 ggtcgcgccc tcggcgcgcg tgggataggg gcgcgcgccc gtcgccagct cgaacaggag
    10081 aacgcccagc gaatagacgt cggaggcggt cgtgatgtcg ccgccgcgaa gctgctcggg
    10141 gcttgcgtac tccggcgtga gcggcgcgcg cgccgtcgtc agttcggaga ccggcgcgcc
    10201 ctcggcggag aagatcttcg cgacgccgaa gtccaggagg cgcggcgttc cctcggcggt
    10261 tacgaggacg ttcgcgggct tgatgtcgcg atggagcacc agccgccggt gcgccgcctc
    10321 cacgctcccg cagagcgcca ggaacaggcg cagccgctcg cgcagggtcg gggccttctc
    10381 cgagcaccac tcgtagaggg gcaccccttc cacgtactcc atgaccagcc acggcagccc
    10441 ctcgggcgtg gtgccgccgt ccaagagccg cgccatgttg ggatgatcca gggtggccag
    10501 gatctgccgc tcccgcagga agcgctggac catctcctgc gtggccagcc cgtgccggat
    10561 gagcttgagc gcgccgcgct ggtggaactg cccgtcctcg cgctccacga gatagaccac
    10621 gcccatcccg ccggtggcga gcggacgcac cactctccac gaaccgatgc tctgtcccgg
    10681 tgcgggacct tcggggaagg ggagcgagat gccgcccccc tccggctcca ggaagccgcc
    10741 cgcggcgtcg tggctcgcga gcagcgagcg cacttcggtc tcgagctcgc gatcgccggc
    10801 gcacgactcc ttgacgaacg cggctcgctg ctcgcgcggc tgccgtagcg ccgcctcgaa
    10861 gagctcaccg acctgcgccc aacgctcggt cacgcgatcc tccctttgag ctcgcgatgg
    10921 agccacgccc gcgcgagggt ccagtcgcgt ttgacggtcg cggcagagac gccgagcacc
    10981 tcggccgtct cctccacgga aagcccggtg aagaagcgga gctcgacgac gcgcgcctgg
    11041 cgctcgtcga gcgcggccag ccggtcgagc gcgtcgttca gatcctccgc cgccacgccc
    11101 aagggctcgt cgcccgcggc gatcgcgtca ctgtcttcga gcgtgacgtg cacggcgtcg
    11161 ccgccgcgct tctgcgcccc gcggcggcgg gcatgatcga tcaggatacg ccgcatcgcc
    11221 tgcgccgcga gccccatgaa gtgggcgcgg ttctgccagg agacgtgccg ctggtcgagc
    11281 aggcggaggt aggcctcgtg ggcgagcgcg gtgggctgca gcgtgtgtcc ggcgctctca
    11341 tctcgtaagt aggcgcccgc aagacggcgc atctcttcgt aaacgagcgg gagcagcgca
    11401 tggagcgcgc ttgcatctcc ttgccgccag cgaagaaagg tttgggtgac ttcagaaggg
    11461 gacggagccc cctgactctc cgggacatcc atcggcccgg ggagtataga ggcgagccgt
    11521 ttcgtggaac aatcgcccgt tatctcgtgc ggcgacccac gggcgcgtgc ccggtgcgcg
    11581 cgaaatcgat gaagccgcgc tcgcagtccg tgtggaccag cttgacccgc acccggtcgc
    11641 ccacgtccag gcccgcctgg ccctgttcga cgcgaccctc gacggccgga tcgagcagcc
    11701 gcacccacgt ccccttgtcg gacgcgccgg tcacgatgcc gtcgaactcc tgccccaggc
    11761 ggctgctcag caggcaagcc gcggccgcct tgtccaggag ccgctcgatc ttgttcacgt
    11821 cgtcttccct ttcggtgcag tggccggcca gcgatcgcag cgcatcgggc gcatagggaa
    11881 ttgcaattcc ctcgagcgcg gcacggagaa gccgttgggt gatcaggtcg gggtagcgcc
    11941 ggttcggagc ggtggagtgc gtgtagtcgc tcacggcgag cccgaagtgc ccctcgatgg
    12001 tctcgccggg aaaggtcgc
//
LOCUS       DASSLZ010000181         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_444759, whole genome shotgun sequence.
ACCESSION   DASSLZ010000181 DASSLZ010000000
VERSION     DASSLZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444759"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFM00_07085"
     CDS             complement(<1..205)
                     /locus_tag="VFM00_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HET7498048.1"
                     /translation="MTGEIQPGPGRYIAVEGVIGVGKTSLARLLAERLQARLLLEEPE
                     ENPFLEDFYKDPRRHAFATQMFFL"
     gene            complement(202..702)
                     /gene="folK"
                     /locus_tag="VFM00_07090"
     CDS             complement(202..702)
                     /gene="folK"
                     /locus_tag="VFM00_07090"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7498049.1"
                     /translation="MMRRVYVGIGSNLGDREFLVRKAVESLRQLPQTNVSGVSSLYDT
                     EPVGETEQPPFLNAVAWIETELLPRELLWQMLLIEKRMGRVRSKRWGPRSIDLDLLFY
                     DDELIDEPDLQVPHPEAHRRAFVLYPLLELDPNFRHPVTGETVRRMIQKLPPNPPVRK
                     LGRFWY"
     gene            complement(699..1067)
                     /locus_tag="VFM00_07095"
     CDS             complement(699..1067)
                     /locus_tag="VFM00_07095"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:NF014233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET7498050.1"
                     /translation="MASGFDWLRLRGIQAYGHLGVTQKERDLGQRLEADVEIAYARET
                     RRRPDSLDAFVDYEELSRLVRGQIAMARCRLLETLAEEVALALLAEFDPPRVRVRLRK
                     MHIPVADFSGCPEVEVERAR"
     gene            complement(1072..2277)
                     /locus_tag="VFM00_07100"
     CDS             complement(1072..2277)
                     /locus_tag="VFM00_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7498051.1"
                     /translation="MPHTEPSERLAALPRYLFAELERKRAEAEAAGREVIDLSIGDPD
                     LPTPAPILEKMAEAARDPRNHRYPTSAGMPAARAEIAAWFGARFGVTLDPRRELGMLL
                     GSKEGIGHLPLALLNPGDEALVPDPGYPVYAAGAVFAGATPVRYPLRESAGFLPEIRE
                     LDRRVTERTRVVWVNYPNNPTGATAPHSFYEELVAWAVKRDLIVASDAAYSELYYDEP
                     PPSILQFPGARERAIEFHSFSKTFNMTGWRIGFAAGAPALIDLLARFKANVDSGAPQA
                     TQIAAAWGLSALPAHGPALRTVYRERRDAALDGLRALGCPVPTPGGAFFVWGRVPEGE
                     SAMDFAARVFEGTGVLLTPGTGFGEGGEGYFRVALTSPVDVIRRAIAKLEALTPWRTR
                     DVSPSAAIR"
     gene            complement(2357..>3020)
                     /gene="murB"
                     /locus_tag="VFM00_07105"
     CDS             complement(2357..>3020)
                     /gene="murB"
                     /locus_tag="VFM00_07105"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET7498052.1"
                     /translation="VAGNRITAGAGCDLGEVVEAAREHSLSGLEFAIDIPGTVGGAIR
                     GNAGAFGRAVGDIVTRIRLLEGTTLVDRTPAELGFAYRHSNLKTNSNIVVDCDFALQP
                     GDRAEMDRIMGQHAAQRARRKEKGLHTAGCFFKNPVLPDGTKIAAGQLLEAAGAKEIK
                     DGGAGVHAYHANYIVNKGGASAQEVLRVAQEMKRRVEEANGITLEEEVMVVMDPPAPG
                     QV"
BASE COUNT          453 a         1040 c         1069 g          458 t
ORIGIN      
        1 ccaggaagaa catctgcgtg gcgaaggcgt gccggcgcgg atccttgtag aagtcctcca
       61 ggaacgggtt ctcctcgggc tcttccagga gaagccgcgc ctggagccgc tcggccagga
      121 gccgggcgag cgaggtcttc cccactccga tcacgccttc gaccgcgatg tagcgccccg
      181 ggccgggctg gatctcgccg gtcagtacca gaagcgaccc agctttcgca cggggggatt
      241 gggcgggagc ttctggatca tgcggcgcac cgtctcgccc gtgaccggat ggcggaaatt
      301 gggatccagc tccagcagcg gatagaggac gaacgcgcgg cggtgggcct cgggatgagg
      361 cacttgcaga tcgggctcgt cgatcagctc atcgtcgtag aagaggaggt cgaggtcgat
      421 ggatcgcggg ccccagcgct tggagcgcac gcgccccatg cgcttctcga tcaggagcat
      481 ctgccagagg agctccctcg gcaggagctc ggtctcgatc caggccaccg cgttcaggaa
      541 ggggggctgt tcggtttcgc ccaccggctc ggtatcgtag agcgaggaga cgcccgagac
      601 gttggtctgc ggcagctgcc gcaatgattc caccgctttc cggaccagga actcgcggtc
      661 gcccaggttc gacccgatgc cgacatagac gcggcgcatc atcgggcgcg ctccacttcc
      721 acttccgggc acccggagaa gtcggccacg gggatgtgca tcttccggag ccgcacgcgc
      781 acacgcggcg ggtcgaattc ggccaggagc gcgagggcca cctcctcggc cagcgtctcg
      841 agcaggcggc agcgcgccat cgcgatctgc ccgcgcacca ggcgcgagag ttcctcgtag
      901 tccacgaacg cgtccagcga atcgggacgc cgccgggtct cgcgcgcgta ggcgatctcg
      961 acgtccgcct ccaggcgctg tcccaggtcg cgctccttct gcgtcacgcc caggtggccg
     1021 taggcctgga tgccgcgcag gcggagccag tcgaacccgg aggccatggc gctagcggat
     1081 cgcggccgac ggggagacat cccgggtcct ccagggcgtg agcgcctcca gcttggcgat
     1141 cgcgcgccga atcacgtcca cgggcgacgt gagcgccacg cggaaatagc cctcgcctcc
     1201 ctcgccgaat cccgtgcccg gcgtgagcag cacgccggtt ccctcgaaga cgcgtgcggc
     1261 gaagtccatc gcggactccc cttccgggac gcgcccccag acgaagaagg cgccgcccgg
     1321 cgtcgggacg gggcaaccca gcgcgcgcag tccatcgagc gccgcgtcgc gccgctcgcg
     1381 atacaccgtg cggagcgccg ggccatgggc cggcagggcc gacagccccc aggccgcggc
     1441 gatctgggtc gcctgcggcg caccggaatc cacgttggcc ttgaaccgcg ccagcaggtc
     1501 gatgagcgcg ggtgctccgg cggcgaagcc gatccgccac cccgtcatgt tgaaggtctt
     1561 ggagaaggag tggaactcga tcgcgcgctc cctggcgccg gggaactgga ggatgctggg
     1621 cggcggctcg tcgtagtaga gctcgctgta ggcggcgtcg ctcgcgacga tcaggtcgcg
     1681 cttcacggcc caggccacga gctcctcgta gaaggagtgg ggtgctgtgg cgccggtcgg
     1741 gttgttcgga taattcaccc acacgacccg cgtgcgctcg gtgacgcggc ggtccagctc
     1801 gcggatctcg ggcaggaagc ccgccgactc ccggagcgga tagcggaccg gggtggctcc
     1861 ggcgaacacc gcccccgcgg cgtagaccgg atacccggga tcgggcacca gcgcctcgtc
     1921 ccctgggttt aggagcgcga gcggaaggtg cccgatcccc tccttggagc ccagcagcat
     1981 ccccagctcg cgccggggat cgagcgtgac gccgaagcgc gcgccgaacc acgccgcgat
     2041 ctccgcgcgc gcagcgggca tcccggcgct ggtcggatag cggtggttcc tcggatcacg
     2101 cgcggcctcg gccatcttct cgaggatcgg cgcgggcgtc ggcaggtcgg gatctccgat
     2161 cgagaggtcg atgacctccc ttccggcggc ctcggcctcg gcgcgcttcc gttccagctc
     2221 ggcgaagaga tagcgcggaa gcgcggcaag gcgctcgctc ggttccgtgt ggggcatgga
     2281 gtgacggtat cggggatcgg ggggagaatc aaggggccgt ccggttcgga agcgaagacg
     2341 gcgacgacgc gcgccgctag acctggccgg gagccggcgg gtccatcacg accatcacct
     2401 cttcttcgag ggtgatgccg ttcgcctctt ccacgcgccg cttcatctcc tgcgcgacac
     2461 gaagcacttc ctgggcggag gcgccgccct tgttcacgat gtagttggcg tggtaggcgt
     2521 gcacgccggc gccgccgtcc ttgatctcct tggcgcccgc ggcttcgagg agctgccccg
     2581 cggcgatctt ggtgccgtcg ggaagcacgg ggttcttgaa gaagcagccg gccgtgtgga
     2641 ggcccttttc cttgcggcgg gcgcgctgcg cggcgtgctg ccccatgatc cggtccatct
     2701 cggcccggtc gcccggttgg agcgcgaaat cgcaatcgac gacgatgttc gagttcgtct
     2761 tgagattgct gtgccggtaa gcgaatccca gctccgcggg ggtgcggtcg acgagggtgg
     2821 ttccctcgag cagccggatc cgcgtcacga tgtcccccac cgcgcggccg aacgcgcccg
     2881 cattaccacg aatagcgcct cccaccgtgc cggggatgtc gatcgcgaac tccagtcccg
     2941 agagggagtg ctcgcgagcc gcctccacga cctcgcccag gtcgcacccc gctccggccg
     3001 tgatgcggtt cccggccacg
//
LOCUS       DASSLZ010000182        11555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_65563, whole genome shotgun sequence.
ACCESSION   DASSLZ010000182 DASSLZ010000000
VERSION     DASSLZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65563"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="VFM00_07110"
     CDS             <1..1590
                     /locus_tag="VFM00_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7498053.1"
                     /translation="DPDGQGMQTEVIRSGKARLFADVRARVRRPGRYFDVDPEGRVHD
                     LSRPGAGPPGAQCAMMAPLKLEGRVVGIVQVMSDMAGTYRRGHLASLEAIVAPIAVAL
                     QNAELYARAKREIAERLRVERALTLSEERLLEADRRKDEFLATLAHELRNPLAPIRNA
                     VGLLRGSGSDPATIAWSGEVIQRQVGHMARLLDDLLDVSRVSRGRLRLRTEPVDLGEV
                     VRHAVEASHPLIEAGQHRLVVEFPPQPIRLDADATRLAQVFSNLLNNAARYSEPGTTI
                     QVSASREEGEAVVRVTDHGIGIMPSMLPRIFEAFLQIDRSLERSQGGLGIGLTLVKQI
                     VELHGGHVEAQSDGPGKGSVFTVRLPLPDPAKAGAGAAEGGPGSARRGDALDAPLAAG
                     VPGSLVAPPPEPRGPAPRRRILVADDLEDSATSLAMLLKMQGHEVRTARDGDEAVGVA
                     RSFLPEIVLLDIAMPKLNGYEAARRILAEHPDKRPVLIALTGWGHEENRMRTREAGFD
                     HHLVKPVEPEVLNRLLATVET"
     gene            complement(1634..2308)
                     /locus_tag="VFM00_07115"
     CDS             complement(1634..2308)
                     /locus_tag="VFM00_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HET7498054.1"
                     /translation="MATPRLAPPRSATHRRTTALVPFAIVIALLWAAWPTPAQAHCDG
                     LDGPVVTAARQALQAGDPNLVLIWVQKKDEPEIRRAFERTTKLRKLGADAKDVADTWF
                     FETLVRVHRAGEGAPYDGLKPAGRDLGPAIPAADKAIATGDVAPLMKLLQRGVEEGLR
                     EQFEKVAALKGYAPGDVEAGRKYVGAYVTYLHYAERLYAASAHPAHGHFAEEEAAADG
                     PHNEEE"
     gene            2484..3083
                     /locus_tag="VFM00_07120"
     CDS             2484..3083
                     /locus_tag="VFM00_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7498055.1"
                     /translation="MSRTIRGGFLVACLLAALALSACGKKTENRGTQNEPLQITDSLA
                     GTPGAAGRPQGSASIPTDLDSMAFRPKLEQVRADIDSGNAQYIQAWKVGSADLFANVF
                     AKEGTLMRRDGNLLQGRGRITAVMGGVFSKIRMRQGEITTRSLRVVGDRAYETGTYLF
                     ETVPVRGGPASVDSGPFVQVWKYESGQWKMWRNDARPRG"
     gene            complement(3090..3740)
                     /locus_tag="VFM00_07125"
     CDS             complement(3090..3740)
                     /locus_tag="VFM00_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7498056.1"
                     /translation="MKIGILGSGSVGKAIGTGFAALGHDVMMGSREPDREDVKEWRKG
                     AKGKTSSGTFADAAAHAELAVLATAWSGTENAITLAKPENLKGKVVIDTTNPLDYSVP
                     GRPPVLAVAGGDSAGENVQRWMPQARVVKAFNIVGNPDMFRPTYPDGPPTMFIAGNDD
                     AAKNEVRQVLEAFGWPDTVDLGGIENARYLEPLAMVWIVNYFRSGKGGSAFKLIRK"
     gene            3909..4652
                     /locus_tag="VFM00_07130"
     CDS             3909..4652
                     /locus_tag="VFM00_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498057.1"
                     /translation="MRSGALSWGWAARLALALLSIVTWTAGSSMAAGAGAGDWNRGSL
                     PPSIRDRVHFAASDVPAFPMDDARLSRAKQWPKTLTIGGVPCALRIHAWDDDDAIAVA
                     SLEDVEEHSRVMLFYDTTRSRWDAANAGWGPRYLWNEKGKLIQRIWYEPDSTRLVTHD
                     YTYYKDGKLLGYSRREEKRRSSRNGASPYQFLSEFFSKDGRLLAVAYENMSARSRDSV
                     YAWKGTTVPYDEFRMKTHVLYSSAHPGSR"
     gene            4723..6342
                     /locus_tag="VFM00_07135"
     CDS             4723..6342
                     /locus_tag="VFM00_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET7498058.1"
                     /translation="MLSLRNASIPALFVLLILATANYAPAPPGVAERVQVRLPGLEQG
                     SLTLCGERLWQPKALARFYKARQARPAWGGRDAEDIVQAIRGVTADGLNPGDYHLKTI
                     EQMVAERENGSTPELEGDLDILLSDAVAAIADHVRYGRVKPVTLNAAWNVDPREGAPP
                     LEQQLAEIRSAPDLSRAIQARRPDHFIYRGLVRELASLRDIEDQGGWPRVASGRPVAP
                     GGRDERIAGVRRRLEASGDYRGGAGADPSHYDASLAKAVRQFQELHRIPPDGVIGPTT
                     VAAMNVPVQARINQVRANLERARWVLNGLHDDFLLVNIPAFKAYLIRDQRRIWEARTQ
                     VGDEYMKTPTFRSMMQTVVFNPDWTVPASIAKYEVLDAMRNGDNVLLKWGLRVYDRRG
                     REVHPASVNWDAPPERFPYTLRQLPGSRNPLGRVKFLFPNKYSIYLHDTPNKRLFAAR
                     DRTFSHGCIRVENAMELADLLLRDQIGTGGVRNAVAEGATRNVALEHPIPVLIVYWTV
                     SVGAKGDVHYANDIYGYDAPLAAALNAGRRG"
     gene            6463..7128
                     /locus_tag="VFM00_07140"
     CDS             6463..7128
                     /locus_tag="VFM00_07140"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7498059.1"
                     /translation="MSRVPLKQLIGIPLNVVLFLFLMVMAWGSWDGFFAHPVRVGVVA
                     IHLLMMPVMTLCTAGRSRGVKHAPDWRPFFPLLMAHSLFTAWVMPYMDARNIGVLPGG
                     DALRWAGLAVLASGALLRVWPMMALGGRFASVAAIQKGHSLQTSGVYGFVRHPSYVGI
                     LLMDIGFAGVFRSSVAVALLPIVFWMFKRRMDVEEQLLVEEFGETYRGYMGRTARLLP
                     GVY"
     gene            7269..7667
                     /locus_tag="VFM00_07145"
     CDS             7269..7667
                     /locus_tag="VFM00_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498060.1"
                     /translation="MKTRMLPLLFLASLVILSFAPTFGQAAEPDHIVSGPEIQARIDQ
                     KIHSEADDRQAIRDLLSRSDVKRVAGQAGLDLRRADAAIGTLSGPELTRIASQARDAN
                     SQLAGGKTITMTWTMVIIVVVALVVLIAVL"
     gene            7682..9823
                     /locus_tag="VFM00_07150"
     CDS             7682..9823
                     /locus_tag="VFM00_07150"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET7498061.1"
                     /translation="MMRLAAAGAILASAALLGIPAFAAEPAPATSPSAGTAVRLLDVP
                     YVPQSERLCGGAALAMVLRYWGDPRARAEDFTSLVLPGENGIRGDSLVAAARARGCPV
                     FPMQATAADLRDHLARGRPVIALLDTGTPVRHYVVLLAATPTGVILHDPAVGPFQTRS
                     AQDFDKAWGAAGRWALLVLPPQGRESLADPSRVALEQFRAGTPAPPPPDTTAFDTPVI
                     PEVATSGPTISGCDGMVSEGVQKAKSGADAEAAGLLNAAAALCPTSAAPVRELAGLRF
                     REKRYADAGSLARRAIELEPNDAYTWRLLAGSRYMDGDVHEALDAWNHVSEPRSDLLK
                     VEGLRRTRYEAVSDQVDLPPGYLITWDEYRRAQRRLNEVPAASRARMEVRPSADGAAQ
                     VRVAVLERPLLFDGPLDLGIAAGRAVASSEVALRLSSPLRLGELWTARYRFQQNRPRG
                     MLMMEAPALGGRPGLFHLEGVWERQAYALQPSVEGARVREDRRRGVVSYGDWISPDWR
                     MEFGGGLERFVVVDAGGHNWATVQGALEAHSVGDHFAFTVSGAGWATGGEHAFRAGDF
                     LVRYGSDDFNHGDWLMRAGYSAASARAPLALWPGASNGSGRDPLLRAHPLLEEGVVNG
                     LVFGKRLAVANFERRIFPWEVKTVRIGLAAFLDTGKAWEPLVPRDIPWQFDVGGGLRI
                     ASVGGRGELRVDFGYGLQDQETAISAGLTAR"
     gene            9837..10289
                     /locus_tag="VFM00_07155"
     CDS             9837..10289
                     /locus_tag="VFM00_07155"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET7498062.1"
                     /translation="MTATLYTIGYEKRTQEEFFTILSQAGIDVLMDVRDVAWSHKPGF
                     SKGTLSETAQTAGLEYVHAQFFGNPKKLREKETETAGLIRAYERHLEEHPEILEEFSR
                     LIRDYAKAEKRVCIMCFERDPADCHRGVLASRWKRRKRGAVEHLGTES"
     gene            complement(10388..11230)
                     /locus_tag="VFM00_07160"
     CDS             complement(10388..11230)
                     /locus_tag="VFM00_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7498063.1"
                     /translation="MFEPLIPPDQIRLLERYEMTCWRTWFMAPGPALIDQLGLRFENL
                     YGAQLAISSKLDHLLFNRVLGLGNEEPVSPAIVDRIIQTYIGSGVPRFFMQVSPVAEP
                     RSLTGWLKDRGFEMIEPWAKLRRGLGPAREIEFAPRIVQIPSSDRDLAKVWGEIVQTA
                     FEYPPETAEWFSELVDLGRWRTYLAYEGDKPVGAAALFVNGEWASFEIGVVLPEAQGR
                     GIHSALIARRIQDAEAAGCKVMTMETSAEKGQGRAQSFRNARRMGFELAYFRENWEWN
                     GAVV"
     gene            complement(11339..>11555)
                     /locus_tag="VFM00_07165"
     CDS             complement(11339..>11555)
                     /locus_tag="VFM00_07165"
                     /inference="COORDINATES: protein motif:HMM:NF041539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor R domain-containing protein"
                     /protein_id="HET7498064.1"
                     /translation="YTLQADSTYWIVVYNAGSGSVGWTTGSPSLTPTGLATFAGARLD
                     SLIAPAPPTSNTNAIGQFSVIGTRINP"
BASE COUNT         1710 a         3951 c         4098 g         1796 t
ORIGIN      
        1 gatcccgatg gccaggggat gcagaccgag gtgatccgaa gcggcaaggc gcggctcttc
       61 gccgacgtcc gcgcgcgcgt ccgccggccg gggcgctact tcgacgtgga ccccgaggga
      121 cgggtgcacg acctgtcgcg accgggagcc gggcctccgg gagcgcagtg cgccatgatg
      181 gcgccgctca agctggaggg gcgcgtcgtg ggcatcgtgc aggtgatgag cgacatggcc
      241 ggcacctatc gccgcggtca tctggcctcg ctcgaggcga tcgtggcgcc gatcgcggtg
      301 gcgctccaga acgccgagct ctacgcgcgg gccaagcgcg agatcgccga aaggctccgc
      361 gtggagcgcg cgctgacgtt gagcgaggag cggctgctgg aggcggaccg ccgcaaggac
      421 gaattcctgg ccacgctggc gcacgagctg cgcaatccgc tcgcgccgat ccggaacgcg
      481 gtcggtctgc ttcgaggaag cgggtccgat cccgcgacga tcgcctggtc gggagaggtg
      541 atccagcgcc aggtgggaca catggcccgg ctgctcgacg atctcctgga cgtgagccgg
      601 gtgagccgcg ggcggctgcg gctgcggacc gagccggtgg acctggggga agtggtgcgg
      661 cacgccgtag aagcgagcca tcccctgatc gaggcgggac agcaccgcct ggtggtcgag
      721 ttccctcccc aaccgatccg gctcgacgcc gatgcgacgc ggctggcgca ggtcttctcg
      781 aacctcctga acaacgcggc ccgctacagc gagccgggca cgaccatcca ggtgagcgct
      841 tcgcgcgagg agggggaggc ggtcgtgcgc gtcaccgacc acgggatcgg catcatgccc
      901 tcgatgctgc ctcgaatctt cgaggcgttc ctccagatcg accgctcgct ggagcgatcg
      961 cagggtggat tggggatcgg gctcacgctg gtgaagcaga tcgtggagct gcacggcgga
     1021 cacgtcgagg cgcagagcga cggcccgggg aaggggagcg tgtttacggt gcggctgccg
     1081 ctgcccgatc cggcgaaggc cggcgcgggt gccgccgagg gcgggccggg gtccgcgcgg
     1141 cgcggcgacg cgctcgacgc ccccttggcc gcgggagttc ccggaagtct ggtcgcgcct
     1201 ccgcccgagc cgcgtggacc cgcgccccgg cgccgcatcc tggtcgccga cgacctcgag
     1261 gacagcgcga ccagcctcgc gatgttattg aagatgcagg ggcacgaagt gcgcaccgcg
     1321 cgcgacggcg acgaggcggt gggcgtggcc cgctccttcc tgcccgagat cgtgctgctc
     1381 gacatcgcca tgccgaagct gaacggctac gaggcggcgc ggcggatcct ggccgagcac
     1441 cccgacaagc ggccggtgct gatcgccctg accggatggg ggcacgaaga gaaccgcatg
     1501 cgcacccgcg aggcgggctt cgaccatcac ctggtcaagc cggtcgagcc ggaagtcctg
     1561 aatcgtctgc tcgcgacggt cgagacgtaa accgccgaag gtccccgagc acgggcaacg
     1621 aaggccggcc ctttcactcc tcctcgttgt gaggaccgtc ggcagccgct tcctcctccg
     1681 caaagtggcc gtgcgcgggg tgcgccgacg ccgcgtagag ccgctcggcg tagtgcaagt
     1741 aggtcacgta ggccccgacg tacttcctgc cggcctcgac gtccccgggc gcgtagccct
     1801 tgagcgcggc gaccttctcg aactgctcgc gaagcccttc ctcgacacca cgctgcagca
     1861 gcttcatcag cggagcgacg tcaccggtcg cgattgcttt atccgcggcc gggatcgccg
     1921 ggccgagatc gcgtcccgcg ggcttcagcc cgtcgtaggg cgcgccttcg ccggcgcggt
     1981 ggacgcggac cagcgtctcg aagaaccagg tatcggcgac gtccttcgcg tcggcgccga
     2041 gcttgcggag cttcgtcgtg cgctcgaacg cgcggcggat ctcgggctcg tccttcttct
     2101 gcacccagat cagtacgagg ttggggtcgc cggcctggag cgcctggcgg gcggcggtca
     2161 cgacgggacc gtcgaggccg tcgcagtggg cttgggccgg ggtcggccag gcggcccaga
     2221 gcagggcgat gacgatggcg aagggaacaa gggcggtggt gcgacggtgc gtcgccgagc
     2281 gcggcggcgc aagacggggc gtggccatgg gatactcctc gacacggacg gcgccgcgtc
     2341 gccgccctgg ttgtgcgcgc gcacttcatt tatacacccg gccacccgcg gcgtcgatgg
     2401 ccgggaagtc ttgaatcgcc cccgcgcgac ggtggagact tggagcggga cgaggcggcg
     2461 aggggccatc gtggaggtgg cacatgagcc gaacgattcg aggcgggttc ctggttgcgt
     2521 gtcttctggc cgcgctggcc ctttccgcat gcggcaagaa gacggagaac cgcggcaccc
     2581 agaacgagcc tctacagatc accgacagtc ttgccggcac gcccggcgcg gccgggcgcc
     2641 ctcagggctc cgcgagcatt cccaccgacc tggacagcat ggccttccgc cccaagctgg
     2701 agcaggtgcg cgccgacatc gattcgggaa acgcccagta catccaggca tggaaggtgg
     2761 ggagcgccga tctcttcgcg aacgtcttcg cgaaggaggg caccctgatg cgccgtgatg
     2821 gaaaccttct ccaggggcgc ggacggatca ccgcggtcat ggggggcgtc ttctccaaga
     2881 tccggatgcg ccagggggag atcacgaccc gctcacttcg ggtcgtgggc gatcgcgcct
     2941 atgagaccgg cacctacctg ttcgagaccg ttcccgtgcg cggcggtccc gcctcggtcg
     3001 actcgggtcc gttcgtgcag gtctggaagt acgagtcggg gcagtggaag atgtggcgca
     3061 acgacgcgcg accgcgcggg tgactcgccc tatttccgga tcagcttgaa cgccgagccg
     3121 cccttcccgc tccggaagta attcacgatc cagaccatcg cgagcggctc gaggtagcgg
     3181 gcgttctcga tcccgccgag atccacggta tccggccatc cgaacgcttc cagtacctgc
     3241 cgcacttcgt tcttggcggc gtcgtcgttg ccggcgatga acatcgtggg cggtccgtca
     3301 gggtaggtcg gccggaacat gtcgggattg ccgacgatgt tgaaggcctt gaccacgcgg
     3361 gcctggggca tccagcgctg gacgttctcc cccgcggagt cgccgcccgc cacggcgagc
     3421 accggaggcc tgccgggcac gctgtagtcg agcgggttcg tggtatcgat gacgaccttg
     3481 cccttcaagt tctcgggctt ggccagcgtg atcgcgttct cggtgccgct ccaggccgtc
     3541 gcgagcaccg cgagctccgc gtgcgctgcc gcgtcggcga aggttcccga ggaggtcttt
     3601 cccttggcgc ccttccgcca ctccttcacg tcctcgcgat ccggctcgcg gcttcccatc
     3661 atcacgtcgt ggccgagcgc cgcgaagccg gtgccgatgg ccttgcccac actacccgat
     3721 cccaggattc cgatcttcat cgctcgccct tcctcaagcg cggcgctgag gccgcgcggt
     3781 tcgtaacggt tcgtcccgga gggacgctgc cgccctcgcg ggcgtggccc ggacgccaac
     3841 tcccgaccga cgctctcctt agacggggag ggcacgtggg ccgattcggg ggctgctatc
     3901 ctttttccat gagatccggt gctctctcat ggggctgggc ggcgcggctg gccctcgcct
     3961 tgctctcgat cgtcacctgg acggcgggat cttcaatggc cgcgggggcg ggcgcggggg
     4021 actggaatcg cggttctctc cccccctcca tccgcgaccg cgtccacttc gccgcgagcg
     4081 acgtccccgc gtttcccatg gacgatgcgc ggctgtcgcg ggcgaagcag tggccgaaga
     4141 ccctgacgat cggcggggtg ccctgcgcgc tccgcattca cgcgtgggac gacgacgacg
     4201 cgatcgcggt cgcgtcgctc gaggacgtcg aggagcatag ccgggtgatg ctgttctacg
     4261 acacgacacg atcgcgatgg gacgcggcca acgccggctg gggtcctcgc tacctctgga
     4321 acgagaaggg gaagctgatc cagcggatct ggtacgagcc ggacagcacc cgtctcgtga
     4381 cccacgacta cacctactac aaggatggga agctcctggg ctattcgcgg cgcgaggaga
     4441 agcgcaggtc ttcgcggaac ggcgcgagcc cctaccagtt cctgtccgag ttcttctcca
     4501 aggatggccg gctgctcgcg gtcgcctacg agaacatgag cgcccgttcc cgcgattcgg
     4561 tctacgcctg gaaggggacg acagtccctt atgacgagtt ccgcatgaag acccacgttc
     4621 tctactcgag cgcgcacccc gggagtcgct gaccgcgtat tcggcgctgt tcggattccg
     4681 gacggccgcg ccgcgcgccg ctccccgcgc gaggagctat cgatgctttc gctacggaac
     4741 gcctccattc ccgcgctatt cgtcctgctc atcctggcca ccgccaacta cgcgccggcc
     4801 cctcccggcg tggccgagcg ggtgcaggtg cgcctgcccg gactggagca gggctcgctc
     4861 accctgtgcg gggagcggct ctggcagccg aaggcgctcg cgcgattcta caaggcgcgc
     4921 caggcccgcc ccgcctgggg cggacgcgac gccgaggaca tcgtgcaggc gatccgcggc
     4981 gtgacggccg acggcttgaa tcccggcgat taccacctga agacgatcga gcagatggtg
     5041 gccgagcggg agaacggctc gacccccgag ctggaggggg acctggacat ccttctctcc
     5101 gacgcggtgg ccgcgatcgc ggatcacgtg cgctacgggc gcgtgaagcc ggtgacgctg
     5161 aacgccgcct ggaacgtgga tccgcgcgag ggggcgcccc cgctggagca gcagctggcc
     5221 gagatccgtt cggcgcccga tctgtcccgc gcgatccagg cgcggcggcc cgatcacttc
     5281 atctaccggg ggctggtgcg cgagctggcc tcgctgcgtg acatcgagga ccagggcgga
     5341 tggccgcgcg tcgcgtcggg acgacccgtc gcccccggcg gtcgtgacga gcggatcgcc
     5401 ggcgtgcggc gccgtctgga ggcgagcggg gactatcgcg gcggggccgg agccgatccg
     5461 tcgcactacg acgcttcgct cgccaaggcg gtccgccagt tccaggagct gcatcggatt
     5521 cccccggacg gcgtgatcgg gccgacgacg gtcgcggcga tgaacgtgcc cgtgcaggcg
     5581 cggatcaacc aggtccgcgc gaatctcgag cgcgcccgct gggtgctgaa cgggcttcac
     5641 gacgacttcc tgctcgtgaa catccccgcc ttcaaggcct acctgatccg cgatcagcgc
     5701 cggatctggg aggcgcggac gcaggtgggc gacgagtaca tgaagacccc gaccttccgc
     5761 tcgatgatgc agacggtggt cttcaacccc gattggacgg tgccggcgtc gatcgccaag
     5821 tacgaggtgc tggacgccat gcggaacggc gacaacgtgc tcttgaagtg ggggctccgc
     5881 gtgtacgacc ggcgcgggcg cgaggtccat cccgcttcgg tcaactggga cgccccgccc
     5941 gagcgtttcc cctacacgct gcggcagctg ccgggctcgc gcaatccgct cggccgcgtc
     6001 aagttcctct tcccgaacaa gtactcgatc tacctccatg acacgccgaa caagcggctc
     6061 ttcgcggcgc gggaccggac cttcagccac ggatgcatcc gcgtggagaa cgccatggag
     6121 ctcgcggacc tgttgctccg cgatcagatc ggaaccggcg gcgtgcggaa cgccgtggcg
     6181 gagggagcga cgcgaaacgt ggcgctggag catccgatcc cggtgctgat cgtctactgg
     6241 acggtgagcg tgggcgccaa gggcgacgtg cactacgcaa acgacatcta tgggtacgac
     6301 gcgccgctgg ccgcggcgct gaacgcgggg cggagggggt agcggcgggc gggcgagcga
     6361 gggccgtagc cccgctcaag atcgggacgg tccggccgat tttcctgggg acccgtcgcg
     6421 ccgggccttt gtgggcccgc gccgtcctcc ggagggctac ccatgtcccg agtcccgctg
     6481 aagcagctca tcggtattcc gctgaacgtc gtgctgttcc tgttcctcat ggtgatggcg
     6541 tggggaagct gggacggctt cttcgcgcat cccgtccggg tgggcgtcgt cgcgatccac
     6601 ctcctcatga tgccggtcat gaccctgtgc accgcgggac ggagccgcgg cgtgaagcac
     6661 gcccccgact ggcgcccctt cttcccgctc ctgatggcgc actcgctctt caccgcgtgg
     6721 gtcatgccgt acatggacgc gcgaaacatc ggggtccttc cgggcggcga tgccctccga
     6781 tgggccggac tggcggtgct cgcctcgggt gccttgcttc gcgtctggcc gatgatggcg
     6841 ctggggggcc gcttcgcgtc ggtcgcggcc atccagaagg ggcactcgct gcagacctcc
     6901 ggcgtgtacg gcttcgtgcg gcatccttcc tacgtaggaa tactgctcat ggacattggg
     6961 ttcgccggcg tcttccgcag ctcggtcgcg gtggcgctcc tgccgatcgt cttctggatg
     7021 ttcaagcgcc ggatggacgt ggaggagcag ctcctggtcg aggagttcgg cgaaacgtat
     7081 cgcgggtaca tgggacggac cgcgcggctc ctgccgggag tgtattgatt ctcgaaacgt
     7141 ttgttccttg acgggggcaa atcctccaca atggtgcggg ggctggaccg tgggtctagc
     7201 ccccgttttg ttcgcggctc acccgcggcg cggccggaca cagagccggc ccgcccagga
     7261 ggatatacat gaagacccga atgctgcccc tgctcttcct cgcttcgctc gtcattctca
     7321 gcttcgcccc gaccttcggc caggcggccg agccggacca catcgtctcc ggccccgaga
     7381 tccaggcccg gatcgaccag aagatccact ccgaagcgga cgaccgtcag gcgatccgcg
     7441 acctgctctc ccggtccgac gtgaagcgcg tcgcgggcca ggccgggctc gacctccggc
     7501 gcgcggatgc cgccatcggc accctctccg gtcccgagct cacgcgcatc gcgtcgcagg
     7561 cgcgcgacgc caattcccag ctcgcgggcg gcaagacgat caccatgaca tggaccatgg
     7621 tgatcatcgt cgtggtcgcg ctcgtggtgc tgatcgcggt tctctgatcc aagccgtgaa
     7681 gatgatgagg ttggctgcgg ccggcgcgat cctcgcgtcg gccgcccttc tcggcattcc
     7741 ggcgttcgcg gcggagcccg cgcccgcgac gtccccgtcc gcggggactg cggtccgcct
     7801 cctggacgtt ccctacgttc cccagagcga acggctctgc ggcggggcgg cgctggccat
     7861 ggtgctccgc tactggggcg atccccgcgc gcgcgccgag gacttcacgt cgctcgtgct
     7921 cccgggggag aacgggatcc gcggcgactc gctcgtcgcg gcggcgcgcg cccgaggctg
     7981 cccggtcttt ccgatgcagg cgaccgcggc cgacctgcgc gatcatctcg cgcgcggccg
     8041 gccggtgatc gcgctcctgg ataccggcac tccggtgcgc cactacgtcg tgctcctcgc
     8101 ggcgacgccg accggcgtga tcctccacga tcccgccgtg ggccccttcc agacgcgatc
     8161 cgcccaggac ttcgacaagg cctggggcgc ggcggggcgc tgggcgctgc tcgtgcttcc
     8221 gccgcagggg cgtgagagcc tcgccgatcc ctcccgcgtg gcgctggagc agttccgcgc
     8281 ggggactccg gctccgccgc cgcccgacac gacggcgttc gacacgcccg tgatcccgga
     8341 agtcgccacg agcggtccca cgatctcggg gtgcgacggc atggtgagcg agggggtcca
     8401 gaaagcgaag agcggcgccg acgcggaggc cgccgggctc ctcaacgccg ccgccgcgct
     8461 ctgcccgacc tcggccgctc cggtgcgcga gctcgcgggg cttcgattcc gcgagaagcg
     8521 ctacgcggac gcgggctctc tggcaaggcg ggcgatcgag ttggagccga acgacgccta
     8581 cacgtggagg ctcctcgccg ggagccggta catggacggc gacgtgcacg aagcgctcga
     8641 cgcctggaac cacgtctccg agccgcgcag cgatcttctc aaggtcgagg gactccggcg
     8701 cacgcgctac gaggcggtgt ccgaccaggt ggaccttccg cccgggtacc tcatcacgtg
     8761 ggacgagtac cgccgcgcgc agcgccgcct gaacgaagtc cccgcggcct cccgcgcgcg
     8821 catggaggtg cgcccctcgg cggacggcgc cgcccaggtg cgcgtcgccg tgctggagcg
     8881 gccgctcctg ttcgacgggc cgctcgatct cggcatcgcg gcgggccgcg cggtggcgag
     8941 cagcgaagtg gcgctgcggc tgtccagtcc gcttcgcctg ggcgagctct ggacggcgcg
     9001 ctatcggttc cagcagaacc ggccgcgcgg gatgctgatg atggaggcgc ccgcgctcgg
     9061 cgggcgtccg gggctcttcc acctggaggg cgtctgggag cggcaggcct acgcgctcca
     9121 gccttcggtg gagggcgcgc gggtgcgcga ggatcgccgg cgcggcgtgg tgagctacgg
     9181 cgactggatc tcgcccgact ggcggatgga attcgggggc ggcctggagc gcttcgtggt
     9241 cgtggacgcc ggagggcaca actgggccac ggtccagggc gcgctcgagg cgcacagcgt
     9301 cggcgaccat ttcgcgttca ccgtgtcggg agccggatgg gccaccggtg gcgagcatgc
     9361 cttccgcgcg ggagacttcc tcgtccgcta cggctcggac gatttcaatc acggggactg
     9421 gctgatgcgc gccggctact cggccgcctc cgcgcgggcg ccgctcgcgc tctggcccgg
     9481 cgcgagcaac ggctcggggc gcgacccgct ccttcgcgcg cacccgctcc tggaggaggg
     9541 cgtcgtgaac gggctcgtct tcggaaagcg gctcgccgtc gcgaacttcg agcggcgcat
     9601 ctttccctgg gaagtgaaga ccgtgcgcat cggcctcgcc gccttcctcg acacgggaaa
     9661 ggcgtgggag cctctggtcc cgcgggacat cccctggcag ttcgacgtgg gcggcgggtt
     9721 gcggatcgcg agcgtcggcg gtcggggcga gcttcgggtc gatttcggat acgggctcca
     9781 ggatcaggag accgcgatct cggcgggact cacggcgcgg tgacggcgac cctgcgatga
     9841 cggcgaccct ctacacgatc ggctacgaga agcgcacgca ggaggagttc ttcaccatcc
     9901 tgagccaagc cggcatcgac gtgctcatgg acgtacgcga cgtcgcctgg agccacaagc
     9961 cgggcttttc caaggggacg ctgtcggaga ccgcgcagac cgccgggctc gaatacgtcc
    10021 acgcccagtt ctttgggaat ccgaagaagc tgcgggaaaa ggagacggaa accgccgggc
    10081 tgatccgcgc ctacgagcgg cacttggagg agcatcccga gatcctcgag gagttctcca
    10141 ggttgatccg ggactacgcc aaggcggaga agcgcgtctg catcatgtgc ttcgagcgcg
    10201 accccgccga ttgccatcgg ggggtgctcg cctcgcgctg gaagcgccgt aagcggggag
    10261 cggtggagca cctgggcacc gagtcatgaa tcggtctcga ggccacgaac cgctgcgcga
    10321 ccagccgccg cacgactagc cgctgcgcaa ccagccgctg cacgactagc cgctgcgcga
    10381 ctagccgcta caccaccgct ccgttccact cccagttctc ccggaagtac gccagctcga
    10441 agcccatcct gcgcgcgttg cggaacgact gcgcgcggcc ctgccccttc tcggccgagg
    10501 tctccatcgt catcaccttg cagccggccg cctcggcgtc ctgaatccgg cgagcgatca
    10561 gggcgctgtg gatgccgcgc ccctgcgcct cggggagcac gaccccgatc tcgaacgagg
    10621 cccactcgcc gttcacgaac agcgcggccg ccccgaccgg cttgtctccc tcgtaggcga
    10681 gataggtgcg ccagcggccg agatccacga gctcggagaa ccactcggcg gtctcgggcg
    10741 gatattcgaa cgcggtctgc acgatctcgc cccacacctt ggccaggtca cggtcggagg
    10801 aggggatctg cacgatgcgc ggggcgaact cgatctcgcg cgcggggccc aggccgcggc
    10861 ggagcttggc ccagggctcg atcatctcga aaccgcgatc cttgagccat cccgtgaggg
    10921 acctgggctc ggcaacgggg ctgacctgca tgaagaagcg gggcacgccc gagccgatgt
    10981 aggtctggat gatccggtcc acgatggcgg gactcaccgg ctcctcgttc cccaggccga
    11041 gcacgcggtt gaagaggaga tggtccagct tggaggagat ggcgagctgg gcgccgtaca
    11101 ggttctcgaa acggaggccc agctggtcga tcagcgccgg tcccggagcc atgaaccagg
    11161 tgcgccagca ggtcatctcg taacgctcga ggaggcggat ctgatcgggg ggaatcaaag
    11221 gctcgaacat ggtcaggcgg ctccttggat ccaacgaatc gtgaagacga gccccgtgag
    11281 gagcagcgga gcgtgcacgc ggcgcgaagc gtacaccgcc ccgcgccgcg cggcatcatc
    11341 aaggattgat tcgcgtgccg atcaccgaga actggccgat cgcgttcgtg ttgctcgtcg
    11401 gcggcgccgg cgcgatcagg ctgtccagcc tggcccccgc gaaggtcgcc agcccggtgg
    11461 gcgtcaagga tgggctgccg gtggtccacc ccaccgatcc cgatcccgcg ttgtagacca
    11521 cgatccagta ggtggaatcc gcctggagcg tgtag
//
LOCUS       DASSLZ010000183        13112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1322502, whole genome shotgun sequence.
ACCESSION   DASSLZ010000183 DASSLZ010000000
VERSION     DASSLZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13112
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322502"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VFM00_07170"
     CDS             <1..1217
                     /locus_tag="VFM00_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HET7498065.1"
                     /translation="DYTVTGTGFSGTMPNVSRPIRRLTFAVSGTRTTATVNLLEAVVG
                     SSTANVTGTMSLGTVRSGNFDVKIDRLVADEWTGGSGTGLSLDAPGGPDQAKPALGVM
                     VANMKIGELRNRGLLIHDVTVPVRFAQGKLVADPIEGTIGKGSVKGALDVTGVGSHPA
                     FTLHLNLNEVPVEDLVSGLLPVRLPVSGSLTDVVDLRGQGYPGPEAAATMTGTVAGKV
                     ERGLLRQNPALKQLREALGAVSPSEVPFQTIAHSARIQGGKLMLDKVAGDLGKDLFSI
                     TGAVGFDQTLDLNALLRLAPSRVEGSTFLAQLASYGKDAEGRVPIEVKITGSALSPKV
                     SVQPAKFIETAGKGFVQEELGKLLTPKPSRTAKTDSAHAGADSAVVTPRRAARDSAAS
                     AVKGALRKLFGK"
     gene            1214..2236
                     /locus_tag="VFM00_07175"
     CDS             1214..2236
                     /locus_tag="VFM00_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7498066.1"
                     /translation="MRAAREGPAIHAADLSREFRAKKAAPVLALDHVDLVVERGECFG
                     LLGPNGAGKSTLIKILTTLLLPSSGRAQVAGYDVERDPIAVRRRINLVSGGDTSGYGI
                     LTVRETLHLFARFYGVPGPVARARADELMAITGLHEKADTRLSSLSTGTKQKLNFARG
                     FMSDPEIIFLDEPTLGLDVEASRQVRSFIARWVRERPHRTVLLTTHYMVEADELCGRV
                     AIIDRGRILALDTPRALKRMVPEEPVFSLSIGAGATDFDALGRIGGVRSLSHHAHPAT
                     GAVEVRVIVEEDQVIGEVLGRLKSSGQPVLHLTKMEPTLETVFIHMVGRGLDDEADGN
                     APAEPR"
     gene            2233..3063
                     /locus_tag="VFM00_07180"
     CDS             2233..3063
                     /locus_tag="VFM00_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7498067.1"
                     /translation="MTRTALAESLRVNAAAILARARVRVVASWRETSWVVGETVFPFL
                     TMSAFVLVYRGLHAPKVYEAFVVLGAAMTAYWNNVLWSMASQFFWEKEQGQLQLYLIT
                     PVSRMSILLGMALGGMAMTTTRAIVILLGGMLLFGIALPADRLLPAFGLFLLTLAAVY
                     ALGMILSSVFLLWGRSAWHTVTLFQEPVYLLSGFYFPVRFLGAAVGALAALLPITLGL
                     DGIRQIFYGKAAHGFVPVVWIAPIEVALFALFLFLAHRALLLMETLGKREGRLTLRGQ
                     "
     gene            3060..3902
                     /locus_tag="VFM00_07185"
     CDS             3060..3902
                     /locus_tag="VFM00_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7498068.1"
                     /translation="MKRSWNVTRAAAWLGWQVEANWADPVLFLAYSFAKPLATTLILF
                     FMVRVVAHGAATRETFLFLFLGNTFFLYVSEVLVGLSWVIFRDREDFETLKYVYLAPI
                     RFLPYLFGRALTKIATATLGTVVALLFGRFALGLPIAASPAALLALLGVVVLGLGGIV
                     FLGVALAGAALIMARHSMNLNEGLTGTFYLLCGAVFPIEVLPRWAERLSLLLPFTYWL
                     ELIRRILTGHAYAAPLRAISDGGLVGILALSTLGLAAVSLLWFRWCEHTARARGLIDW
                     KTNY"
     gene            complement(3906..4406)
                     /locus_tag="VFM00_07190"
     CDS             complement(3906..4406)
                     /locus_tag="VFM00_07190"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7498069.1"
                     /translation="MGTLKQRRQKQLAMVGAIGHEHFMTGVRMLYACRYQDALQHFQE
                     ASMLEPNARGYLSYLGLATAHAQRKYSDAEQLCRRAIEAEYHRPEHYFNLAEVFSLAG
                     RRGDAIKAYNQALSWNPNYEPAQDALRKMGVRRPPILGSLPRSHPLNVFLGKTFRRRS
                     VPKSAR"
     gene            4665..5159
                     /locus_tag="VFM00_07195"
     CDS             4665..5159
                     /locus_tag="VFM00_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7498070.1"
                     /translation="MASVVAPITAPPAVELHLRSDLFISDLRTRKKASVLEEIAGALA
                     ATKVARTPEAILDALRRREALGSTGLGKGIAVPHARSTMVSERALLVARSSKGIEFDA
                     VDGQPVHLCFLIVAPPLERDPIYLKLVAEIVRATRLARTRQKLIDAPDFAALRTILVE
                     AARD"
     gene            5152..5502
                     /locus_tag="VFM00_07200"
     CDS             5152..5502
                     /locus_tag="VFM00_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498071.1"
                     /translation="MTDLLDALVELQDIDVLLREVKDPEIASQEERMGFSLEHVENLE
                     KARRKVASQIDEKLLQTYERMSRRFARVVVPVEGIVCAGCRMSLPTASARRHSSVAVG
                     IENCENCGRILYRR"
     gene            5535..5957
                     /locus_tag="VFM00_07205"
     CDS             5535..5957
                     /locus_tag="VFM00_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498072.1"
                     /translation="MPIRHTFLALAAFAALALAGAGPTLTARAEPPTQFVGPTQILFV
                     DADSVYWLRSAEWNSAYVRNQEKLLLHSLRMKYWRDHLPADMRLVFETLGYPVGRVIH
                     TPEGHVEEWWTYRLLEPPLRFRDGVLIDTDRFEALRSR"
     gene            6052..6594
                     /locus_tag="VFM00_07210"
     CDS             6052..6594
                     /locus_tag="VFM00_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498073.1"
                     /translation="MTCSRIPSDSRVPPGRTVAAAWMLAAVVALFAAPSALSAGAEPP
                     QGGWAVIAQADTSGNPNGTQGDEGDEEEDNGVKIPPNLLEPEPGTVPDTTGAPGQNVL
                     PMSPAPSDSGRAIVPVNPALPETIRYVQPTDPAVKGKAPAPNLVPVQPKRKGGIFGLG
                     PAVVILSLAVVHFFVVKAVK"
     gene            6594..7349
                     /locus_tag="VFM00_07215"
     CDS             6594..7349
                     /locus_tag="VFM00_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498074.1"
                     /translation="MALRRHPGSGTLDALRVRWHPRDFPLRGAAETVLRAAVRELSLE
                     HVVRDLHVRVDLDNRDDHAYIEWNTHDHRAARLSFAIGNFVTRARRRAWSRTWGKRPG
                     TPPLTPRQFSAKSFSEACLHELSHLRDDHQSGVDLTGAPASDREGLNELWNVWIDGRL
                     NRRGLPAMTRGERRRVYVRTLRGTPRFRARGERVFQALWNADHLGPRELRAYLAELTT
                     PLRGAVTPAARPRRPKTTRPKARVKITRSRRTR"
     gene            7346..9127
                     /locus_tag="VFM00_07220"
     CDS             7346..9127
                     /locus_tag="VFM00_07220"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7498075.1"
                     /translation="MSEETLPGAAGLLTEEERLRFLVRASRELALLKRDALARRARDL
                     FLGAGSFSEFSLWVLDPRTELLYPWTLCNTPVVGRRPRLTPGQGLAGQACADEAPAFH
                     AELPEALYTPFEWGGRPRAQLRGVLTVPLRQGAEPLGVVVMLGDAASAGVAPASEEIA
                     FFGELGAQLSIAVGNARIYADAMREKVQNQLLLELGTRISGSLDTERLMEQILDLVFQ
                     VVRYDAAGIYLVDKKTQWITRQTIRGYDPARDDAVRLKVGSGLIGWVAKAGQGVIVAD
                     VRNDPRYRNARDETRSEMVAPIRIGSEVIGAFNLESDEPDAYEVEDMELLMSFASQAA
                     VAIERTRLHEEVLEKRRLDEEITIGQRIQRSFLPARNPEVRNFDIAGANYSSDRVGGD
                     YYDFIRIADEHLGVVVGDVSGKGIAAALIMAAFRASLIAEIRNNYAIRTTMAKVNRLL
                     WESVEPDRFVTSLFGVLDTPARRFTYVNAGHNPALLYRAATGQFDTLEPTGPLLGTFE
                     AATYKERVVEIRPGDVLVMYSDGVTEAMAPSGEQFGEERLRKEIEARKTEPATVQLRG
                     IWEAVQAFQGGEQDDDVTIVVVRGMAA"
     gene            9562..10392
                     /locus_tag="VFM00_07225"
     CDS             9562..10392
                     /locus_tag="VFM00_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7498076.1"
                     /translation="MTQKIEILQVNGARIAYQVEGSGEAVVLVHAGVANMSMWDDQVA
                     GLRDRYRVIRYDCRGYGRTSTEKVPFSNRDDIVAVMDHLGESSAHVVGLSRGGQIALD
                     FAIEKPARVRSLVFAAGGLSGYESPAWKPEEFEEPQRLEEAKDWEGLARWETQHWVNG
                     PGQPADRVDPALRSRVHDWILSNYRAEKEPGEPQPLRPPAVGRLSELQAPLLVMVGTL
                     DEPETVDTCRYLAAHVPGARLEIFEGAAHMLNLEQPERFTRLIREFFASAARVSPARR
                     "
     gene            10441..10527
                     /locus_tag="VFM00_07230"
     tRNA            10441..10527
                     /locus_tag="VFM00_07230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10475..10477,aa:Leu,seq:caa)
     gene            complement(10563..12185)
                     /locus_tag="VFM00_07235"
     CDS             complement(10563..12185)
                     /locus_tag="VFM00_07235"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7498077.1"
                     /translation="MPRITHPIKTTLPTLDDARIFPLPGDPEPAPAEARPEAGYRSLL
                     RERRTRDVILDQLPPHRALEELQQILSGTADPEVRAWCYLRAGQLRERAGSLAAAQSH
                     YEEAVALEPEDPEVAYFAWNNVAYCLNRAGRNHEAEPAARRARDLDPKRWNAHKNLGV
                     ALEGLGRNVEAAESHLAAAKMAPEHPVPLRRLRMLLARDAAIAREHPGLAQDVERLVH
                     NTWGDRRPSAPPDSDILERELRDRLGRIEVDEAVVHGDFQIFGLRWMVAQPLAYRTLD
                     EALSARALQITEVSEQGSVPRIRVRNAGTDRIFLMAGEQFVGAKQNRVLNASILVDAH
                     ADLQLPVSCVEQGRWAYRDVRFTSKGSSHYALRHLMSKKAHAHYKVSGTPDSDQREVW
                     REVARKLGDHDTASPSGALDDVYTKLGRNLDQAVEPIQSGEDWCGAAFAFGGRIVGVD
                     LFDKPATLRLLWPKLTRAYALDVMGKTRVSDLSKTWVESWIHDAAHSLIDFFPSPGAG
                     IDCRFESNHHAGAVLIVDGCPVHLEMFHEEEM"
     gene            12401..>13112
                     /locus_tag="VFM00_07240"
     CDS             12401..>13112
                     /locus_tag="VFM00_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7498078.1"
                     /translation="MLFASAGRSMGSVVLNRLRVTVACVFLGTALFLLRGSPWPFWAT
                     PLQIALLAVSGLIGFVFGDRYFFKSLVILGPSRATLLVSFGPIFTALIAWPVLHEHPG
                     PYALLGMALTLSGVAFVLSGRARRKMVHVEGSAFAGVVAGLLGTLGQAGGYVISKAAL
                     RKGLDPLSATVIRVSAAMIGIWILAASTGKVAQTVSALRDRRASAFMVGGAFLGPFLG
                     VTLSLTALKYIQAGVAASI"
BASE COUNT         2001 a         4487 c         4449 g         2175 t
ORIGIN      
        1 tggactacac ggtgaccgga accggcttca gcgggacgat gccgaacgtc tcccgtccga
       61 tccgaaggct gacgttcgcg gtctccggga cgcggacgac cgccaccgtg aatttgctgg
      121 aggcggtggt cggctccagc accgccaacg tgacggggac gatgtccctg ggcacggtgc
      181 ggagcggcaa cttcgacgtg aagatcgacc ggctggtggc ggacgagtgg accggcggaa
      241 gcggcaccgg gctgagcctc gacgcgcccg ggggcccgga ccaggccaag cccgcgctgg
      301 gcgtgatggt cgcgaacatg aagatcggcg agctgcgcaa tcgcggcctc ctgatccatg
      361 acgtgaccgt tccggtccgc ttcgcccagg gaaagctggt ggccgacccg atcgaaggga
      421 cgatcgggaa gggctccgtg aagggcgcgc tggacgtgac gggcgtgggc agccaccccg
      481 ccttcacgct gcacctgaac ctgaacgagg tgccggtgga ggacctggtc tcgggacttc
      541 tccccgtgcg gctccccgtc tcgggcagcc tgacggacgt cgtggacctg cgcggccagg
      601 gctacccggg tcccgaggcg gcggcgacca tgacgggcac ggtggccggc aaggtcgagc
      661 ggggcctctt gcggcagaac cccgccctga agcagcttcg cgaggcgctg ggcgcggtct
      721 ccccgagcga ggttccgttc cagacgatcg cgcacagcgc gcgcatccag ggcgggaagc
      781 tgatgctgga caaggtggcg ggcgacctcg ggaaggacct cttctcgatc accggagcgg
      841 tcggcttcga tcagacgctg gatctcaacg cgctgctccg gctcgcgccc tcgcgcgtgg
      901 aggggagcac cttcctcgcg cagctcgcca gctacggcaa ggacgcggag ggacgggtgc
      961 cgatcgaggt gaagatcacc ggcagcgccc tctcgcccaa ggtgagcgtg cagcccgcga
     1021 agttcatcga gacggcgggg aaggggttcg tccaggagga gctcgggaag cttctcacgc
     1081 ccaagccctc ccgcaccgcg aagaccgaca gcgcgcacgc gggcgcggac agcgccgtcg
     1141 tcaccccgcg gcgcgcggcg cgcgactcgg ccgcgagcgc cgtcaagggc gccctccgga
     1201 agctgttcgg gaagtgaggg ccgcccggga aggcccggcc atccacgcgg ccgacctctc
     1261 gcgggagttc cgcgccaaga aggcagcccc cgtgctggcg ctcgatcacg tggacctcgt
     1321 ggtcgagcgc ggcgagtgct tcggcctcct cggccccaac ggggcgggga agagcaccct
     1381 catcaagatc ctgaccacgc tcctgctccc gtcctcgggg cgcgcccagg tagccggcta
     1441 cgacgtggag cgcgatccga tcgcggtccg ccgccggatc aacctggtct ccggcgggga
     1501 cacgtcgggc tacggcatcc tgacggtgcg ggagacgctc cacctcttcg cccgcttcta
     1561 cggcgtcccg gggcccgtcg cgcgcgcccg cgccgacgag ctgatggcga tcacgggcct
     1621 gcacgaaaag gcggacacgc ggctctccag cctctccacc gggacgaagc agaagctcaa
     1681 cttcgcgcgc ggcttcatga gcgatcccga gatcatcttc ctggacgagc cgaccctggg
     1741 cctggacgtg gaggcctcgc gccaggtgcg ctccttcatc gcccgctggg tacgcgagcg
     1801 cccgcatcgc acggtgctcc tcacgacgca ctacatggtc gaggcggacg agctgtgcgg
     1861 acgggtggcg atcatcgacc gcgggcgcat cctcgccctg gacacgccgc gcgcactgaa
     1921 gcggatggtg ccggaggagc cggtcttctc gctctcgatc ggcgcggggg cgaccgactt
     1981 cgacgcgctg ggccggatcg gcggcgtgcg atcgctctcg catcacgccc accccgcgac
     2041 gggtgcggtg gaggtccggg tgatcgtgga ggaggaccag gtgatcggcg aggttctcgg
     2101 ccgcttgaag tcctcggggc agccggtgct tcacctcacc aagatggagc ccacgctgga
     2161 gaccgtcttc atccacatgg tggggcgcgg gctggatgac gaggcggacg ggaacgcccc
     2221 ggcggagccc cgatgacgcg cacagcactc gccgaatcgc tccgcgtgaa cgcggccgcc
     2281 atcctggccc gcgcccgcgt gcgcgtggtc gcctcgtggc gcgagacctc gtgggtggtc
     2341 ggagagacgg tgtttccgtt cctcacgatg tcggcgttcg tgctcgtcta ccgcggcctg
     2401 catgcgccca aggtgtacga ggcgttcgtc gtgctgggag ccgcgatgac ggcctactgg
     2461 aacaacgtcc tctggagcat ggcgagccag ttcttctggg agaaggagca gggacagctg
     2521 cagctgtacc tgatcacgcc ggtgtcgcgg atgagcatcc tgctggggat ggcgctgggg
     2581 ggaatggcga tgaccacgac ccgcgcgatc gtgatcctcc tcggcggcat gctcctcttc
     2641 ggcatcgcgc tcccggccga ccgcctcctg ccggcgttcg gcctgttcct gctcacgctg
     2701 gccgcggtct acgcgctggg gatgatcctg agcagcgtct tcctgttgtg ggggcgctcc
     2761 gcgtggcaca cggtgacgct cttccaggag ccggtctatc ttctctcggg gttctacttc
     2821 ccggtgcgtt ttttgggtgc ggcggtgggc gcgctcgcgg cactcctgcc gatcacgctc
     2881 gggctggacg ggatccgcca gatcttctac ggcaaggcgg cgcacggctt cgtcccggtg
     2941 gtgtggatcg cgccgatcga ggtcgcgctc ttcgccctct tccttttcct cgcgcaccgg
     3001 gcgctcctgc tgatggagac gctggggaag cgggaagggc ggctcacgct gcgcggccaa
     3061 tgaagcgctc ctggaacgtg acgagggcgg ccgcctggct gggatggcag gtggaagcca
     3121 actgggccga cccggtcctg ttcctcgcct attccttcgc caagccgctc gccacgacgc
     3181 tgatcctctt cttcatggtg cgcgtcgtgg cgcacggcgc ggccacgcgc gagacctttc
     3241 tcttcctctt cctcgggaac acgttcttcc tctacgtgag cgaagtgctg gtcggtctct
     3301 cgtgggtgat cttccgggac cgggaggact tcgagacgct gaagtacgtc tacctcgcgc
     3361 cgatccgctt cctgccgtat ctcttcgggc gcgcgctcac gaagatcgcc accgcgacgc
     3421 tcggcaccgt cgtggccctc ctcttcggcc gcttcgcgct cggacttccg atcgcggcct
     3481 cgccggcggc gctgctcgcg ctcctcggcg tcgtcgtgct ggggttgggc gggatcgtct
     3541 tcctcggcgt ggcgctcgcg ggcgccgcgc tgatcatggc gcggcacagc atgaacctga
     3601 acgaggggct gacgggaacg ttctacctgc tctgcggcgc cgtcttcccg atcgaggtcc
     3661 tgccgcgatg ggcggagcgc ctctcgctcc tgcttccgtt cacctactgg ctcgagctga
     3721 tccgccggat cctgacgggg cacgcctacg ccgcgccgct gcgcgcgatc tccgacggag
     3781 gactggtcgg catcctcgcg ctctccacgc tggggcttgc ggccgtgtcg cttctctggt
     3841 ttcggtggtg tgagcacacc gcgcgggcgc gcgggctgat cgactggaag acgaactact
     3901 gagggtcagc gggcgctctt gggtaccgat ctccggcgga acgtttttcc caggaagacg
     3961 ttcaggggat gcgaacgggg cagcgagccc aggatcggcg gccgccgcac gcccatcttg
     4021 cgaagcgcgt cctgcgccgg ctcgtaattg ggattccagg agagggcctg gttgtaggcc
     4081 ttgatcgcgt cgccgcgccg tcccgccagc gagaagactt cggccaggtt gaagtagtgc
     4141 tcgggacggt ggtactcggc ctcgatcgcg cggcggcaga gctgctcggc gtcggagtac
     4201 ttccgctggg cgtgggcggt cgcgagcccc aggtagctca ggtagccgcg ggcgttcggc
     4261 tccagcatgg acgcttcctg gaagtgctgg agcgcgtcct ggtagcggca ggcatagagc
     4321 atgcgaaccc cggtcatgaa gtgctcatga ccgatcgcgc cgaccatcgc cagctgcttt
     4381 tgccgccgct gcttcagtgt ccccatccgg ccctcctcgc ctcgtgagga ggcaaggcaa
     4441 gccgcgtgcc cgaaccgacc acttcatgac gattgcgaca tggctggaac cagtaccgcc
     4501 gcatggtgtt aaacgattcg tgtgatgcac tgcgcgaggc gaaatgcacg aagccgccgc
     4561 gcgcgccccg caaacaacga tcttgccatg gacggattgc gtcttgtaat gtcctggggc
     4621 ggggaaccct gaacctccca ccccgaggag tcgccaagag ccgcatggct tccgtcgtcg
     4681 caccgatcac cgcaccgccc gcagtcgaac tgcatcttcg atccgaccta ttcatctcgg
     4741 accttcgcac tcggaaaaag gcttcggttc tcgaagagat cgccggcgcg ctcgcggcca
     4801 ccaaggtggc ccgcacgccc gaggcgatcc tggacgctct ccggcgccgc gaggcgctcg
     4861 ggagcaccgg gctcggcaag gggatcgcgg tgccgcacgc gcgctccacg atggtctccg
     4921 agcgcgcgct tctcgtggcg cgctcctcca agggaatcga attcgacgcg gtggacgggc
     4981 agcccgtgca cctttgcttc ctgatcgtcg cccctcctct ggagcgcgat cccatctacc
     5041 tcaagctggt ggccgagatc gtgcgtgcga cccgccttgc ccggacccgc cagaagctga
     5101 tcgacgcccc cgatttcgcc gcgcttcgca ccattctcgt ggaggctgcc cgtgactgat
     5161 ctcctggacg ccctggtgga gctgcaggac atcgacgtgc tgctccgcga ggtcaaggat
     5221 cccgagatcg cctcccagga agagcggatg ggcttctccc tggagcacgt ggagaacctc
     5281 gagaaggcgc gccgcaaggt ggcctcccag atcgacgaaa agctcctcca gacctacgag
     5341 cgcatgagcc gccggttcgc ccgggtcgtg gttccggtgg agggcattgt ctgcgcggga
     5401 tgccggatga gcctcccgac ggcctccgcc cgcaggcatt cttcggtcgc cgtaggtatt
     5461 gaaaactgcg agaattgcgg acgaatcctc taccgtcgct agaagtctcc cgccgttcgc
     5521 ggtataatca cttcgtgccg atccgacaca cattcctcgc gctcgcggcg ttcgccgccc
     5581 tggctctggc cggtgccggt ccgaccctga ccgcgcgagc cgagccgccc acccaattcg
     5641 tcgggcccac ccagatcctg ttcgtggacg ccgactcggt ctactggctc cgctcggcgg
     5701 aatggaactc ggcctacgtc cggaaccagg agaagctgct cctccattcc ctccggatga
     5761 agtactggcg ggaccatctg ccggccgata tgcgcctggt cttcgagacg ctcggatatc
     5821 cggtcggacg ggtgatccac accccggaag ggcatgtcga ggagtggtgg acctatcgcc
     5881 tgctcgagcc cccgcttcgc ttccgcgacg gggtcctgat cgacaccgac cgcttcgaag
     5941 cgctccgctc ccgctgagct ccggcgcgcc gcccgcgccg ttcccgacga ctccgatttt
     6001 ttcggccccg cccgcgcccg cgtgcgccct attttcgtgt aggatcgctt cgtgacctgt
     6061 agtcggattc cctcggattc gcgcgtgccg cccggccgga cggtcgccgc ggcgtggatg
     6121 ctggcggcgg tcgtggcgct gttcgccgca ccttcggcgc tgtccgcggg cgccgagcct
     6181 ccgcagggcg gatgggccgt gatcgcgcag gccgatacga gcggcaatcc gaacgggacg
     6241 caaggggacg agggtgacga ggaggaggac aacggcgtga agattccccc gaacctgctg
     6301 gagcccgagc cgggcaccgt gcccgatacc acgggcgcgc ccggacagaa cgtcctcccc
     6361 atgagcccgg ctccgtccga ctcgggaaga gcgatcgtgc ccgtcaatcc ggccttgccg
     6421 gagacgatcc ggtacgtcca gccgaccgat cccgccgtga aggggaaagc cccggcgccc
     6481 aacctggtgc cggtccaacc caagcgcaag ggcggcatct tcggactcgg accggccgtg
     6541 gtcattctga gcctcgccgt ggtccatttc ttcgtcgtca aggcggtgaa gtgatggccc
     6601 ttcgccgcca tcccggaagc ggaacgctcg acgcgctgcg cgtgcgctgg catccgcgcg
     6661 acttcccgct ccgcggcgcc gccgagacgg tgctccgcgc ggcggtgcgc gagctctccc
     6721 tggagcacgt ggtgcgcgac ctgcacgtcc gcgtggacct ggacaaccgc gacgaccacg
     6781 cctacatcga gtggaacacg cacgaccatc gggccgcgcg gctctcgttc gcgatcggga
     6841 atttcgtgac gcgggcgcgc cggcgcgcct ggtcgcgcac gtggggcaag cggccgggaa
     6901 cgccgccgct caccccgcgg cagttctccg ccaagagctt ctccgaggcc tgcctgcatg
     6961 agctctcgca ccttcgcgac gaccaccagt cgggcgtcga cctgaccggc gctcccgcgt
     7021 cggaccgcga ggggttgaac gagctctgga acgtctggat cgacggccgg ctgaaccgcc
     7081 gcggccttcc ggccatgacg cggggcgagc gccgccgcgt ctatgtccgg accctgcgcg
     7141 gcacgccgcg cttccgcgcg cgcggcgagc gcgtcttcca ggcgctctgg aacgccgatc
     7201 atttggggcc gcgcgagctg cgcgcctatc tcgccgagct cacgacgccg ctccgcggcg
     7261 ccgtgacgcc cgccgcgcgc ccgcgccgcc cgaagacgac ccgaccgaaa gcccgcgtca
     7321 aaatcacccg gagccgccgg acccgatgag cgaagagacg ctccccgggg ccgccgggct
     7381 cctcaccgaa gaggagcgcc tgcggttcct cgtgcgcgcc tcgcgcgaac tcgcgctgtt
     7441 gaagcgcgac gccctggcgc ggcgggcgcg cgacctcttt ctgggtgcgg ggtcgttctc
     7501 cgagttctcc ctctgggtcc tcgatccgcg caccgagctg ctctatccct ggacactctg
     7561 caacacgccc gtcgtcggtc gccgcccacg gctcacgccg gggcaggggc tggcggggca
     7621 ggcctgcgcc gacgaggcac ccgcgttcca cgccgagctg cccgaggccc tctacacgcc
     7681 cttcgagtgg ggcggccgcc cgcgcgcgca gctccgcggg gtgctcaccg tgcccctccg
     7741 ccagggcgcc gagccgctcg gcgtggtcgt gatgctgggt gacgccgcca gcgcgggcgt
     7801 ggctcccgcc tccgaggaga tcgcgttctt cggcgagctg ggggcgcagc tttcgatcgc
     7861 ggtcggcaac gcgcgcatct acgccgacgc catgcgcgag aaggtgcaga accagctctt
     7921 gctcgagctc gggacgcgca tctcgggaag cctggacacc gagcgcctca tggagcagat
     7981 cctggatctc gtgttccagg tcgtgcgcta cgacgccgcg ggcatctacc tcgtcgacaa
     8041 gaagacccag tggatcacgc gccagaccat ccgaggctac gatcccgcgc gcgacgacgc
     8101 cgtgcggctc aaggtgggaa gcggcctgat tggctgggtg gccaaggcag ggcagggcgt
     8161 gatcgtggcc gacgtgcgga acgacccgcg ctatcgaaac gcgcgcgacg agacgcgaag
     8221 cgagatggta gctcccatcc ggatcgggag cgaggtgatc ggcgccttca atctcgagtc
     8281 cgatgagccc gacgcctacg aggtcgagga catggagctc ttgatgtcct tcgccagcca
     8341 ggccgcggtg gccatcgagc gcacgcgcct ccacgaagag gtgctcgaga agcggaggct
     8401 ggacgaggag atcacgatcg gccagcggat ccagcgctcc ttcctcccgg cccggaaccc
     8461 cgaggtgcgg aacttcgaca tcgcgggcgc caactactcc tccgaccgcg tcgggggcga
     8521 ctactacgac ttcatccgga tcgccgacga gcacttaggc gtcgtggtcg gcgacgtctc
     8581 gggcaagggg atcgcggcgg ccctgatcat ggccgcgttc cgcgcgtcgc tcatcgccga
     8641 gatccggaac aactacgcca tccgcaccac catggccaag gtgaaccggc tcctctggga
     8701 gagcgtcgag cccgaccgct tcgtgacctc gctcttcggc gtgctcgaca cgccagcgcg
     8761 gcggttcacg tacgtgaacg ccggccacaa cccggcgctg ctctaccggg cggccacggg
     8821 acagttcgac accctggagc cgaccgggcc cctcctcggc acgttcgagg ccgcgaccta
     8881 caaggagcgg gtggtggaga tccggccggg ggacgtcctg gtgatgtact ccgacggggt
     8941 caccgaggcg atggccccga gcggggagca gttcggcgag gagcgacttc ggaaagagat
     9001 cgaggcccga aagaccgagc ccgccaccgt ccagctccgt ggcatctggg aggccgttca
     9061 ggcgttccag ggcggggagc aggacgacga cgtgacgatc gtggtggtgc gggggatggc
     9121 ggcctagaac gcggccgaaa acaagaacgg cgggctggtc aggcccgccg atctatttaa
     9181 ttggaagttc cccgataaaa ccgtgcctaa aagacttgtt gaggtcttgg gcaacccacc
     9241 ggcgaacgcc gggtcaagcc acttaggaaa cgcgaatccc agggaacttc acaacccgcg
     9301 ttcgtcactt tagactcagc gagccccgaa aagttccagg ggggaccgaa gctccgaaca
     9361 acatgaggaa ttgtagctaa acacttgtgc cgctgtcaat tgaaatgtct caatctagag
     9421 tggagcgccg accgatttca ggctgtgccg cttagtgtaa ctactacaat agcttagggc
     9481 ttcgcacgga tcgcgccgct cgcggacgtt ctgccgcgag gacgcgattc atctgcggcc
     9541 ccctacgggg aaaccaacac aatgacccag aaaatcgaga ttcttcaggt gaacggcgcg
     9601 cggatcgcct accaggtcga gggaagcggc gaggcggtgg tcctcgtcca cgccggtgtc
     9661 gcgaacatga gcatgtggga cgaccaggtc gcgggtcttc gggatcggta tcgcgtgatc
     9721 cgctacgact gccgaggcta tggccgtacc tcgacggaga aggtgccgtt ctcgaaccgc
     9781 gacgacatcg tcgccgtcat ggatcacctg ggcgagtcct cggcgcacgt cgtggggctc
     9841 tcccgcggcg gccagatcgc cctcgacttc gccatcgaga agcccgcccg cgtccggtcg
     9901 ctggtcttcg cggcgggcgg gctgagcgga tacgaatcgc ccgcgtggaa gcccgaggag
     9961 ttcgaggagc cgcagcgcct cgaagaggcc aaggactggg aagggctcgc caggtgggag
    10021 acccagcact gggtgaacgg ccccggccag ccggcggacc gcgtcgaccc cgcgctgcgg
    10081 tccagggtcc acgactggat cctgagcaac taccgggcgg agaaggagcc gggtgagccg
    10141 cagccactcc gtccccccgc cgtcgggcgc ctctccgagc tccaagcgcc gctcctcgtc
    10201 atggtcggta ccctggacga gcccgagacg gtggacacgt gccgctacct cgcggcccac
    10261 gtgcctggcg cgcggctcga gatcttcgag ggggcggccc acatgctgaa cttggagcag
    10321 cccgagcgtt tcacgcgcct gatccgcgag ttcttcgcct ccgccgcgcg cgtgtcgccg
    10381 gccaggcgat aggtctgcta ccctagccga ttacatcccg caagcagacg gttcgcggtc
    10441 gccggagtgg tgaaattggc agacgcgccg gactcaaaat ccggtcctcg caagagggtg
    10501 ggggttcgat tcccccctcc ggcaccatga ctcagcccgc tctgggccgt gtgccccagt
    10561 tcctacattt cctcctcgtg gaacatctcc aggtgcaccg gacagccgtc gacgatcagc
    10621 accgcgcccg cgtggtgatt gctctcaaag cggcaatcga tgccggcacc gggggacggg
    10681 aagaaatcga tgagcgaatg ggctgcgtcg tggatccatg attcgaccca agtcttcgag
    10741 agatcggata cgcgcgtctt gcccatgacg tcgagcgcat acgctcgagt cagcttcggc
    10801 cagagcagcc ggagcgtggc gggcttgtcg aataggtcca caccgacgat gcgacctccg
    10861 aatgcgaacg cggccccgca ccaatcctcg cccgactgga tcggttcgac cgcctgatcg
    10921 aggttccggc ccagcttggt gtagacatcg tccagggcgc cggaagggga cgccgtatcg
    10981 tggtcgccca gtttccgcgc cacctctcgc cagacttcac gctggtcgga gtcgggcgtg
    11041 ccgctcacct tgtagtgggc atgcgccttc ttcgacatca ggtggcggag cgcatagtgc
    11101 gaggagccct tgctcgtgaa ccggacatcg cgataggccc atcgcccctg ctcgacgcag
    11161 ctgaccggca gttgaaggtc ggcatgagca tcgacgagga tgctcgcgtt gagtacccgg
    11221 ttctgcttgg cccccacgaa ctgctcaccc gccatcagga agatccggtc cgtgccggca
    11281 ttgcggaccc ggatccgagg gactgagcct tgttcgctca cctcggtaat ctggagggcg
    11341 cgggcggaga gcgcctcgtc cagagttcga taggcgagag gctgcgccac catccaccgc
    11401 agtccgaaga tttgaaaatc tccatgcacg acggcctcgt ccacctcgat gcgaccgagc
    11461 cgatcccgca gctcccgttc gaggatgtcg gaatcgggcg gcgccgatgg ccgtcgatcc
    11521 ccccacgtat tgtggaccag gcgttcgacg tcctgggcga gacccgggtg ctcgcgcgcg
    11581 atcgcggcat ccctcgccag gagcatccga agccgccgaa ggggaaccgg atgttccgga
    11641 gccatcttcg cggccgcgag atgggactcc gcggcttcga cgttccttcc caggccttcc
    11701 agggccacac ccaggttttt gtgcgcgttc caccgctttg gatccaggtc acgcgcacga
    11761 cgcgcggccg gctccgcctc gtggttgcgt cccgcgcggt tcaggcaata ggcaacgttg
    11821 ttccaggcga agtacgccac ctcgggatcc tcgggctcca gcgcgaccgc ctcttcataa
    11881 tgggactgag cggccgccag actcccagcc cgctcccgaa gctgtccggc ccgcaggtaa
    11941 caccaggcgc gcacctccgg gtcggcggtg cccgacaaga tctgctggag ttcctccaac
    12001 gcgcgatgag gcggcaactg gtcgagaatc acatcgcggg tgcgccgttc tcgaagaaga
    12061 ctgcgatagc cggcctctgg ccgggcttcc gcgggagccg gttccggatc accagggagg
    12121 ggaaaaatgc gtgcgtcatc gagtgtgggg agcgtcgttt tgatggggtg ggtgatccgt
    12181 ggcatggcgg gactccttcc tggagacgca cgttcgtctc cggaggcctt ggaactgagg
    12241 ggagtatgat cgaccgcgag aaccacggta cacccggctg ggctggtttc gccagaccgc
    12301 ctcgccacga gttaggctgc atggaggatt ctctctgctc cattcgctga ccggtcaatt
    12361 cgcggcgctc ggcacggccc tttgctgggc ttcgggctcg atgctctttg cgtcggccgg
    12421 gcggtcgatg gggtcggtcg tcctcaatcg cctccgcgtc accgtggcgt gcgtctttct
    12481 cggcaccgcc ctgttcctcc ttcgaggctc accgtggccg ttctgggcga cgccgcttca
    12541 gatcgcgctg ctcgcggtga gcggcttgat cgggttcgtg ttcggcgacc gctacttctt
    12601 caagtcgctc gtcatcctgg gtccgagccg cgccacgctc ctcgtctcct tcggcccgat
    12661 cttcaccgcc ctcatcgcct ggcccgtgct gcacgagcat cccgggccct acgcccttct
    12721 tgggatggcc ctgacgctct cgggggtggc cttcgtgctg agcggcaggg cgagaaggaa
    12781 gatggtccac gtcgagggat ccgcgttcgc cggggtcgtg gctggacttc tgggcacgct
    12841 gggtcaggcg ggcgggtacg tcatctccaa ggcggccctt cggaagggac tcgatccgct
    12901 ttccgccacc gtgatccgcg tgagcgccgc gatgatcggg atctggatcc tcgcggcttc
    12961 gacggggaag gtcgcgcaga cggtgtcggc gctccgggac cgcagggctt ccgcgttcat
    13021 ggtgggaggc gcgttcctgg ggccgttcct cggagtcacc ctctcgctga cggccctcaa
    13081 gtacatccag gcgggggtcg ccgcctcgat ca
//
LOCUS       DASSLZ010000184        17242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1458991, whole genome shotgun sequence.
ACCESSION   DASSLZ010000184 DASSLZ010000000
VERSION     DASSLZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 17242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17242
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458991"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VFM00_07245"
     CDS             complement(<1..1187)
                     /locus_tag="VFM00_07245"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET7498079.1"
                     /translation="MPIRDPLFHPAFLGRRAAMETPPFEPFPPTGRSPWSGPDHPRPD
                     LEFHVRHYRTALEVDLERKELRGRVALTLESLREGLREAALDAAELRIASVRAGRKSL
                     AYVLEGERVRITLPRALGVGERVAVEIAYATNPRKGFTFVGPTEAEPERTLGGWSQGQ
                     ADDTHWWMPCLESTESRATHEQIVTAPAGYRVIGNGRLIGKKPNRRARTVTWHWKLDT
                     THPAYLTSLVVGKYVELRDRAGSTPLFYYVPRGKERAARAAFRKTPRMIANFTRVFGH
                     PYAFPKYAQTLVADFTFGGMENTSATTLFDRMLEMPNDSLDEGYDMLVAHELAHQWWG
                     DLVTCRDWSEGWLNEGFATYSEIVTREADAGQEDADFGRLEQMCSYLVEDGCEYRRAL
                     VERR"
     gene            1265..2248
                     /locus_tag="VFM00_07250"
     CDS             1265..2248
                     /locus_tag="VFM00_07250"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET7498080.1"
                     /translation="MDLSHALIAMGRVFADAAVEIVPLFLLAILIGAWIEEYVSERTI
                     TRFLTGRHPATMLLASMTGAVIPLCTCGMVPLAVSLRRRGSDLKHTFAFLTAGASVSV
                     PVLLLTGTVLGARWALVRFVASVLFGLAVGYAAVRALRSVADRSAEQADRVQHGDHTH
                     GPGGITATRPGPEPDGEEFDMAEIRGRSRFASVLRRFRGQVVEYGPWVLVSLAVAAVV
                     DVLVPRHWVDVLYGERTFAGSLLAALSGLPFYFCSGAELPLVRELLLKGMGPGPAAAM
                     LLSVPIVNLLTFGVVSRWIGTRGAFVYLALCVVFSAVLGEITGLAWQAFAT"
     gene            complement(2245..2913)
                     /locus_tag="VFM00_07255"
     CDS             complement(2245..2913)
                     /locus_tag="VFM00_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498081.1"
                     /translation="MQEIHWIDRESGTLVQQQRGQIMRGMAIDPRGDQLVATQLLRVP
                     LRWRPPLADHHEEIPVGNALWEAAAFTRDAQRLILSRRPLTAGSVEDTILVLNAESFE
                     LERSISIPQWITRLRLAADDRVPLVLGQRGITALDFESGRELWSCSKAGFGIDLTPDG
                     RHAITLDRGEWVAFGALTGQETRWPGRTTQDHAWSVSVAPDGTAVSFLDERLIITTIP
                     PLAG"
     gene            complement(3007..3732)
                     /locus_tag="VFM00_07260"
     CDS             complement(3007..3732)
                     /locus_tag="VFM00_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7498082.1"
                     /translation="MSDSLRDKVAIVTGSTKGIGLSIAKALLAEGARVAVSARNRAEV
                     DAVVKDLERQHPGRVLGQTCDVRREDDVRALFDAVDAKWKGLDVLVNNAGIGLFKNVE
                     EIALEEWNAVIETNLTGVFLCTRAAIPRMRARGGGYIFNLSSLAGKTSFPSAAAYNTS
                     KFGLNGFSEAVMQEIRYDGIRVSYLMPGSVNTYFNGHEPDPSQGWKIQPEDIGEIVVD
                     LLRFPGRSLQSRVEIRPSQPPRK"
     gene            complement(3729..4391)
                     /locus_tag="VFM00_07265"
     CDS             complement(3729..4391)
                     /locus_tag="VFM00_07265"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7498083.1"
                     /translation="MKSAPSRFVKPVHFEGPEGRLEGLWDDPAQGLAPAVICHPHPAH
                     GGSMHSKVVYTVFRVLHDAGHATLRFNFRGVGASQGSYSGWDGEVGDVAAAAAFARRE
                     TGITPLWIAGFSFGSWVGSKWAIADPNIERMILLGAPVDRNVDDRSFDHLTRLPAPLL
                     IIQGERDQYGSPEGVARLVERLRPLGSVEAKIVPGADHFFTGHLAPLEAALRQGLGME
                     ET"
     gene            4462..6570
                     /locus_tag="VFM00_07270"
     CDS             4462..6570
                     /locus_tag="VFM00_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HET7498084.1"
                     /translation="MALRADTATFYGLNRSDLVDIYRVMLLSRRMDDEEIKLKKQNLI
                     YFQISGAGHEAILTAAGKALRPGHDWFYPYYRDRALMLCLGMTPLEMLYEAVGAKDDP
                     NSGGRQMPCHWGLKRANVVSQSSPTGTQFLQAVGCAEGGRLLATIQEIAEKRPYHEDE
                     LVYVSAGDGTTSEGEFWEAINSAAVAQLPVLFLITDNQYAISVPVEAQTPGGDIGRCM
                     SSIPGLRVMKCDGTDPLESFKTVTEAAHHLRAGRGPVLLHALVIRPYGHSMSDDEVSY
                     KPQSEREAEAKRDPVHTYPEFLIKEGLATKEQIEKIQKDVEAEIAEAEAAALAAPKPA
                     ADSLYEHLYSPTVDPTSAAFQTEPRFEGQPETMVTLINRCLKDEMSRDARIVLFGEDV
                     ADATREQALNSVPGKGGVFKVTYGLQKQFGKRRVFNSPLAEANIVGRAVGMATRGIKP
                     VVEIQFFDYIWPAMMQIRNELAMLRWRSNGDFACPLVIRVAIGGYLQGGSIYHSQSGE
                     SIFAHMPGLRIVYPASAVDANGLLRTSIRCDDPVLFLEHKNIYRQPYAKGPYPGPDYM
                     IPFGKARVAREGKDVSVITWGATVNRAMQAAQKLEVEGISVEVVDLRTLLPFDREAIA
                     ATVRKTARALVLHEDVLTGGFGGEIAAVIAQDHFESLDAPVRRVGAADTPVGYAPVLE
                     DAILPQVDGITRAIRELARY"
     gene            complement(6608..9082)
                     /locus_tag="VFM00_07275"
     CDS             complement(6608..9082)
                     /locus_tag="VFM00_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7498085.1"
                     /translation="MRFPLGTNVGPYQVVAFVGAGGMGEVYRARDPRLGRDVAVKTIG
                     SGVALEPERLRRFEQEVRAAGTLNHPNVVTVYDVGESEGTPYLVSEFLDGRSLRERIA
                     GSGFTVRQAIDCIAQIARGLEAAHARGIVHRDLKPENVILTSEGRAKILDFGIAKLVR
                     DGTDGPGVTRTRATEGTEAGALIGTTRYMAPEQLRGEAVDARTDLFALGAMLYELITG
                     SHPFARETPTETAAAILTQEAPALPEDVSAQMPGLARLIRKCLEKRPADRFHCAADLA
                     FFLEHLAEDMRAGSAAASEEPAIHSLRRLTFRRGIVSSARFVSDGNSVVYTAAWEGGG
                     KELFWVHTSNPDSRPLRLERADVQAISATGEMALVLNRRFYGGYIYDGTLARLPMAGG
                     TPRPLLDNVASADWAPDGRTLAIVRHKEGKAILEYPVGTVLHETLGWIGSPRVSPDGA
                     RVAFIDHDYRADDAGRICIAERGKEIRVISTDFASAQGLAWGRDGKSLWCTAAPMGAN
                     RSLMRFSLDGSSRTILRVPGSLTLQDLSPRGDVLVVHGHERAGIIAKAPGEDRERDLS
                     WYDWSLMRAITADGSTVLFDESGEGGGQHRLVCIRGTDGSPAIAIGEGSAQDITPDGT
                     WVLSVNIADPHCFTVIPTGIGEPRTISLGDLMFHAGAFFPDGSRAAIIAADAQGSMRP
                     YLVDLVTGERRLFSETDVGAGIPQVSPDGSWIAMSGQDFIVRLHPAAGGDPVVIPGMA
                     RGERVMGWGADSRQVYAFLPGPPPVQVYRIDVKTGQREPHLVAQPPDPTGIEGVMGVR
                     QARDGDGYAYTYVVIMTELYLLEGAA"
     gene            complement(9110..9403)
                     /locus_tag="VFM00_07280"
     CDS             complement(9110..9403)
                     /locus_tag="VFM00_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HET7498086.1"
                     /translation="MPKYVIERDIPKIGGATAKDLMAISQKSCGVLSDMGPKIQWIHS
                     YVTADKIYCVYHAENAEMVREHAKRGGFPANRIEEVKAIIDPASSEQSAMVRL"
     gene            complement(9450..9974)
                     /locus_tag="VFM00_07285"
     CDS             complement(9450..9974)
                     /locus_tag="VFM00_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7498087.1"
                     /translation="MGLEYTPELSIPLLERTPAALRALLSGLPDAWVRATDGPGTWSA
                     FDVVGHLLHGEKTDWIPRVKHILQKGDSEPFPPFDREAMFEATRGRSLASLLEEFTAL
                     RAQNVTTLRGLAMTPADLDRRGLHPELGPVTLRQHLATWTAHDLGHLRQIAQTLGRQY
                     REAVGPWRAYLSMM"
     gene            complement(10007..11509)
                     /locus_tag="VFM00_07290"
     CDS             complement(10007..11509)
                     /locus_tag="VFM00_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7498088.1"
                     /translation="MSATPAPESAPSAAPPLRPAASTILISREVGLKLLWVRRSEANP
                     FLGGFHSFPGGRYAHEDGPLGEDPDENLRTMARCAAREAFEETGLLLGFHGSAPQLEE
                     QRRHRRDVLDGKSAFWETVERRWGLRFDPARYVPCGRWITPHFSRARFNTNFFLIDMP
                     EPSEPDVWPGELESGAWVDPAVALKMWDEDRIVLAMPTLYVIQRLAQGGHGLPGRLHD
                     IPEANGVPSRYVHVRPAITMIPLKTETLPPATHTNAVVVGDSDVVIVDPGSDNPDELE
                     ALYQVVEEAEGSRGRVLAILLTHRHRDHFAGVEAVRARYACPVWAHADVAERIPIDRA
                     LAEGDRIELSGRHPRRLCVLATPGHSRSHLAFFEETSRTLIAGDVVSGLGTVIIAPPD
                     GNLRDYLATLTRLRRIGITALIPGHGAPNRGVARMLDALIEHRRLREGKILRVLEAGP
                     LGEEDLRLRVYDDTPTADPALSARTLLAHLEKLEEEGRVARENGLVRATG"
     gene            complement(11506..12414)
                     /locus_tag="VFM00_07295"
     CDS             complement(11506..12414)
                     /locus_tag="VFM00_07295"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET7498089.1"
                     /translation="MAPHPIHQRLERGELVLLDGALGTELERRGVATPLPLWSAQAIL
                     DAPDTLRQVHEDYARAGADVLAADTFRTTPRVMAKVGRPAGDADRLTEIALRLAAEGR
                     DRGAAGRTVWIGGSLGPLEDCYRPEDAPPQEAMEREHDDQAKRLARSGAEILLLETFN
                     RIDEATAAARAARATGLPFTVSFVCRGGARLFSGEPLADAVRAVEPLLPAAVLVNCTP
                     LDETAACLDVMSRATHLPFGGYPNAGVVGPTGAWSFDPSVTPERFAALAKDWMRLGAQ
                     VLGGCCGTSPEHIRALRESLPLVLVE"
     gene            complement(12449..13630)
                     /locus_tag="VFM00_07300"
     CDS             complement(12449..13630)
                     /locus_tag="VFM00_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7498090.1"
                     /translation="MTNSHPDFGSSAEARAAWRRSTSGATAATRRKRIALSLSGGGLA
                     GAYYEIGCLAALDDFLGFDFSSTGFDIYIGVSAGATVAGLLAKGIPIRKTYWALLTNS
                     DSSLHFKRGDMYDVNRWAILFSGIKMGLSMPGYFVECLRQRVRPSPFEFMHYVEERLP
                     AGLFALDNYQRFFAKVLEAHGKTDSFADIERELYIPATDLDLGTRRVFGAVDAENVPL
                     SHAVAASSAIPLFFRPFKVEGRDYIDGSTGKVAHLDVAIKAGASTILIINPLVNISND
                     QEAVCIPRRDGKCARIKEKGMSFIHDQASRIETRVRLDGEIRRYQAEFPEVKMYRIEP
                     SSSEVILFLHNTMSVQEREAILRYGYTSMTSILKRRRAVIQELMQFHGLNPADEALAG
                     G"
     gene            complement(13627..14424)
                     /locus_tag="VFM00_07305"
     CDS             complement(13627..14424)
                     /locus_tag="VFM00_07305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7498091.1"
                     /translation="MELKGRTVLLTGASGGIGRPAALRLAAAGARVALFARSEAPLAA
                     LAAEVAAAGGEALAVSGDVTRAEDCARAVAATTARFGALDALVNIAGIGYLRGLTEAT
                     DAEIGQMVEVNLLGTVRMTRAALPTLLGRPGAAIVNIGSMAGRVAPVHYGYYSATKFA
                     ISGLTESWRRELGPRGVRVTLLLPAAVETPFVDRAGRERALGSGPAGTILRPEQVAAA
                     LVHALHRNPPDHYVPSWNRAFAWVNLMFPGISDRVMNALFRYPDRER"
     gene            complement(14465..16588)
                     /locus_tag="VFM00_07310"
     CDS             complement(14465..16588)
                     /locus_tag="VFM00_07310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET7498092.1"
                     /translation="MPKRPVTPETLFDIQFLDAVSPSPDGTRAVYEIKTIDREKDEYQ
                     SHLWLIPTGGGAPRRLTQGEHKNFGAVWDPDGTTIAFVSTRRDKKPQIYRLPLDGGEA
                     ERLTDLDGEPSSLEWSPDGASILFVFRASDPPELGHLPGSAPEKKAREAKADKKELPE
                     PTFRHITRMLYKLDGSGWLPRSRSHLHVLDVATRAVRALTSGNFDHGPGAWSPDGKQI
                     AFTANRQEDEEYSSYVVSDVFVVPASGGEPRNLTPGPGVAGGLSWSPDGSTIAFLGHD
                     QPDDWWGIPNYHLWAVPAAGGAPRDLTAGWDRYASDLVGSDLRDFHAPGNPVWSPDGS
                     TLYFQGSEFGSTHIYRVPARGGTPETLTQGGVQILDMKRGRRAEEMIVIRCDHQDAGT
                     VARFHPANGRFTDLVTPNRELFDSLALSAPEERWTEAPGGHRVQSWLIPPSAAAKKTA
                     KHPMILEIHGGPRVQYGACFFLEFQMFASAGFYVLFSNPRGSQGYGEAFTKAIVHDWA
                     APAFDDLMLVVDDALARHPDIDVERLGVTGGSYGGYMTNWVVGHTERFRAAITQRCVT
                     TIPTLLLGDDLCPVATPEFGAEPWEDAEILRRQSPLTYVEKIRTPLLITHSLMDQRCD
                     VTEAEMLYKALKALRREVEMVLFPGESHGLSRKGTPSRRVARMHLMRDWFVRYLAPEG
                     APAPVSGVATSAEPAMAGARQNAAG"
     gene            complement(16598..>17242)
                     /locus_tag="VFM00_07315"
     CDS             complement(16598..>17242)
                     /locus_tag="VFM00_07315"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET7498093.1"
                     /translation="GEETVAALARTFAETAGQPLAGRLIDCLAVAQAAGGDKRGQQSA
                     AILVVRPSDEFPEYRERYVDLRVEDAKEPIKELVRVFGILEAQRLAEAHLRFAEMYDK
                     KGKKELARIERERVGEALQRALDRGENDASNLNGLAWTCATNDVYLDQALQAAQRAVD
                     LEPKNTGILDTLAEVHFRMGHADKAVEVESRALAMEPGNNYLTDQLRRFQSGQK"
BASE COUNT         2536 a         5968 c         6060 g         2678 t
ORIGIN      
        1 cgccgctcca ccagcgcgcg ccggtactcg cacccgtcct cgacgagata agagcacatc
       61 tgctccaggc gcccgaagtc ggcgtcctcc tgccccgcgt ccgcctcgcg cgtgacgatc
      121 tcggagtagg tggcgaagcc ctcgttcagc catccctccg accagtcgcg gcaggtgacg
      181 aggtcgcccc accactggtg cgccagctcg tgggcgacca gcatgtcgta gccctcgtcc
      241 agcgagtcgt tcggcatctc gagcatgcgg tcgaagaggg tggtggcgct ggtgttctcc
      301 atccccccga atgtgaagtc ggcgaccaag gtctgcgcgt acttcggaaa cgcgtagggg
      361 tggccgaaca cccgcgtgaa gttcgcgatc atgcgcggcg tcttccggaa cgccgcgcgc
      421 gcggcccgct ccttgccgcg cggcacgtaa tagaagagcg gcgtggatcc cgcgcggtcg
      481 cgcagctcca cgtacttgcc gacgacgagc gaggtgaggt aggcggggtg cgtggtgtcc
      541 agcttccagt gccaggtcac cgtgcgggcg cggcgatttg gcttctttcc gatcagccgg
      601 ccgttgccga tgacgcggta ccccgccggc gccgtgacga tctgctcgtg cgtggctcgg
      661 ctctcggtgc tctccaggca gggcatccac cagtgggtgt cgtccgcctg gccctggctc
      721 cagccgccca gggtgcgctc cggttcggcc tcggtcggac cgacgaaggt gaaccccttc
      781 cggggattcg tggcgtacgc gatctcgacg gcgacccgct cgccgacgcc cagagccctg
      841 ggaagcgtga tccggaccct ctccccttcg agaacgtagg cgaggctctt cctgccggcg
      901 cgcaccgagg cgatccgcag ctccgccgca tcgagcgctg cctcgcgaag gccctcgcgc
      961 agcgactcca gggtcagcgc gacgcggccc cgcagctcct tccgctccag atccacttcg
     1021 agcgccgtcc ggtagtgacg gacgtggaat tccaggtcgg ggcgcggatg gtcgggaccg
     1081 gaccacgggg agcgcccggt gggcggaaag ggctcgaacg ggggcgtttc catggccgcc
     1141 cggcggccca gaaatgcggg gtggaacagc ggatcacgga tcggcatcag tcccctccac
     1201 gttggagccg catcgtggcc gaggggggcg caacctgtca agcaagctgg ggtagaatcc
     1261 cgggatggac ctctcccatg ccctgatcgc gatggggcgc gtcttcgccg acgccgccgt
     1321 cgagatcgtt cctctcttcc tcctcgccat cctgatcggc gcctggatcg aggagtacgt
     1381 ctcggagcgg acgatcacgc gatttttgac cggccgccac cccgcgacga tgctcctcgc
     1441 ctccatgacc ggcgccgtga tcccgctctg cacgtgcggc atggtgccgc tggcggtgtc
     1501 cctgcgccgg agagggagcg acctcaagca tacgttcgcc tttctgaccg cgggcgcctc
     1561 ggtcagcgtg cccgtgctgc ttctcacggg gaccgtgctc ggggcgcgct gggcgctggt
     1621 gcggttcgtc gcgtcggtgc tgttcgggct ggccgtgggc tacgcggcgg tgcgggcgct
     1681 tcgaagcgtg gcggaccgct cggcggagca ggcggaccgg gtgcagcatg gggaccacac
     1741 acatgggccc ggcgggatca cggcgacgcg gcccggaccg gagcccgacg gcgaggagtt
     1801 cgacatggcc gagatccgcg gacgttcgcg cttcgcctcg gtgcttcgcc gcttccgcgg
     1861 ccaggtcgtc gagtacggac cctgggtgct ggtgagcctc gcggtcgcgg cggtcgtgga
     1921 cgtgctggtg cctcgccact gggtggacgt gctctacgga gagcggacgt tcgcgggatc
     1981 tttgctcgcg gcgctgagcg gcctcccctt ctacttctgc tccggagcgg agctgccgct
     2041 ggtccgggaa ctcttgctga aagggatggg acccggcccc gccgcggcca tgctgctctc
     2101 cgtgccgatc gtgaacctcc tcacgttcgg cgtggtgtcg cggtggatcg gcacccgcgg
     2161 cgcgttcgtg tacctggcgc tctgcgtggt gttctcggcg gtcctggggg agatcacggg
     2221 gctcgcctgg caggccttcg cgacttaacc cgcgaggggc gggatcgtcg tgatgatgag
     2281 ccgctcgtcg aggaaggaca cagcggtccc gtccggagcc acggacacgc tccacgcgtg
     2341 gtcctgcgtc gtgcgccccg gccagcgcgt ctcctgcccc gtaagcgcgc cgaacgcgac
     2401 ccattcgccg cggtccaacg tgatcgcgtg acggccgtcc ggcgtgaggt cgatgccaaa
     2461 tcccgccttg gagcacgacc acaactcgcg acccgattcg aagtccaacg cggtgatccc
     2521 acgttgccct aagacgagcg ggacgcgatc gtcggccgcg aggcggaggc gcgtgatcca
     2581 ttgcgggatg gagatcgagc gctccagctc gaacgactcc gcgttcagga ccaggatcgt
     2641 gtcctcgacc gagcctgcgg tcaacgggcg ccggctcaga atgagacgct gggcatcccg
     2701 ggtgaaggca gccgcttccc acagggcgtt accgaccgga atctcctcgt ggtgatccgc
     2761 gagtggcggc cgccagcgga gcggcaccct caataactgg gtcgccacga gttgatcgcc
     2821 cctggggtcg atggccatcc ctcgcatgat ctgcccgcgc tgctgttgga ccagggtccc
     2881 ggactcccgg tcgatccagt ggatctcctg catgcttcgc tctcgaccgc acggggcccg
     2941 gaggtcggat ggaggccgca tcgccgaggt ccgcctcgcg cagatcctcc aggtcctcgg
     3001 gactcgtcac ttcctcggcg gctggctcgg gcggatctcc acgcggctct ggagcgaccg
     3061 ccccgggaag cgcagcaggt cgaccacgat ctcgccgatg tcctcgggct ggatcttcca
     3121 gccctgcgag ggatcgggct cgtggccgtt gaagtaggtg ttcacgctgc cgggcatcag
     3181 gtagctcacg cggatcccgt cgtagcggat ctcctgcatc accgcctcgc tgaatccatt
     3241 gagcccgaac ttggaggtgt tgtaggcggc cgcgctgggg aaggacgtct tccccgccag
     3301 gctcgagaga ttgaagatgt agccgccgcc ccgcgcgcgc atccggggaa tcgccgcccg
     3361 cgtgcagagg aagacgccgg tgaggttggt ctcgatcacg gcgttccact cctccagcgc
     3421 gatctcctcg acgttcttga aaaggccgat ccctgcgttg ttcacgagaa cgtccagccc
     3481 cttccacttc gcgtccaccg cgtcgaacag cgcgcgcacg tcgtcctcgc gacggacgtc
     3541 gcaggtctgt ccgagcacgc ggccggggtg ctggcgctcc aggtccttca cgaccgcgtc
     3601 cacttccgcg cgattccgcg ccgagaccgc cacgcgcgcc ccctcggcga ggagcgcctt
     3661 ggcgatcgag agcccgatcc ccttggtgct gccggtcacg atcgcaacct tgtcccgcaa
     3721 cgagtcgctc acgtctcctc cattcccaac ccctggcgca gcgcggcctc caggggagcc
     3781 aggtgtcccg tgaagaaatg atcggccccg gggacgatct tcgcctcgac cgagccgagc
     3841 gggcggagcc gctcgaccag gcgcgcgacc ccctccgggc tgccgtactg gtcccgctcc
     3901 ccttggatga tcaggagcgg cgccgggagc cgggtcaaat ggtcgaagga gcggtcgtcc
     3961 acgtttcggt ccacgggagc gcccaggagg atcatccgct cgatgttcgg atccgcgatc
     4021 gcccacttgg agccgaccca cgacccgaac gagaatccgg cgatccagag gggggtgatc
     4081 ccggtctccc gccgcgcgaa cgccgcggcc gcggccacgt cccccacctc gccgtcccat
     4141 ccggagtagc tcccctggct ggccccgacc ccgcggaagt tgaagcgcag cgtggcgtgc
     4201 cccgcgtcat gcagcacgcg gaacaccgtg tagaccacct tgctatgcat ggagccgccg
     4261 tgggccggat ggggatggca gatcacggcg ggagcgagcc cctgagccgg gtcgtcccac
     4321 agcccttcca ggcggccctc cggcccctcg aaatggaccg gcttgacgaa gcgcgacggg
     4381 gcggacttca cgctcgggag tctctccgct atacttgtgg ggctcaagtc gtaattcccc
     4441 ccgtcgagga ggttctcttc cgtggcgctc cgggccgata ccgctacgtt ttacgggctc
     4501 aaccgatccg acctggtcga catctaccgc gtgatgctcc tctcgcgccg catggacgac
     4561 gaggagatca agctcaagaa gcagaacctc atctatttcc agatctcggg cgcgggccac
     4621 gaggcgatcc tcacggccgc cggcaaggcg ctgcgcccgg gccacgactg gttctatccc
     4681 tactaccgcg atcgggcgct gatgctctgc ctgggcatga ccccgctgga gatgctgtac
     4741 gaggcggtgg gcgccaagga cgatcccaac tcgggcggcc gccagatgcc ctgccattgg
     4801 ggactcaagc gggcgaacgt cgtttcccaa tccagcccca ccgggaccca gttcctccag
     4861 gccgtcggct gcgccgaggg agggcggctc ctcgccacga tccaggagat cgcggagaag
     4921 cgcccctacc acgaagacga gctggtctac gtttcggccg gcgacggcac cacgagcgag
     4981 ggggagttct gggaagcgat caacagcgcc gcggtggctc agcttcccgt gctcttcctg
     5041 atcaccgaca accagtacgc gatctcggtt ccggtcgagg cgcagacgcc gggcggcgac
     5101 atcgggcgct gcatgagctc gatccccggt ctgcgcgtga tgaagtgcga cggcaccgac
     5161 ccgctcgagt cgttcaagac ggtgaccgag gcggcgcacc acctgcgcgc cgggcgtggt
     5221 cccgtcctcc ttcacgcgct ggtgatccga ccctacggcc actccatgtc ggacgacgag
     5281 gtctcgtaca agccgcagtc ggagcgcgag gcggaagcca agcgcgaccc ggtccacacc
     5341 tatcccgagt tcctgatcaa ggaggggctc gcgaccaagg agcagatcga gaagatccag
     5401 aaggacgtgg aggcggagat cgccgaggcc gaggcggccg cgctcgccgc gcccaagccg
     5461 gccgcggatt ccctctacga gcacctctac tcgcctacgg tggatcccac gagcgccgcg
     5521 ttccagaccg agccgcgctt cgaggggcag ccggagacga tggtcacgct gatcaaccgc
     5581 tgcctcaagg acgagatgtc ccgcgacgcg cgcatcgtgc tgttcggcga ggacgtggcc
     5641 gacgccaccc gggagcaggc gctgaattcg gtccccggca aggggggcgt gttcaaggtg
     5701 acctacggtc tccagaagca gttcgggaag cggcgcgtct tcaattcgcc gctcgccgag
     5761 gcgaatatcg tgggccgcgc ggtcggcatg gccacgcggg ggatcaagcc agtggtggag
     5821 atccagttct tcgactacat ctggcccgcc atgatgcaga tccgaaacga gctggcgatg
     5881 ctccggtggc gctcgaacgg cgacttcgcc tgcccgctcg tcatccgtgt ggcgatcggc
     5941 ggctatctcc agggcggctc gatctatcac agccagagcg gcgagagcat cttcgcgcac
     6001 atgccggggc tacgaatcgt ctaccccgcg agcgccgtgg acgcgaacgg gctcctgcgc
     6061 acgtccatcc gctgcgacga tccggtgctc ttcctggagc acaagaacat ctaccgacag
     6121 ccctacgcca agggccccta ccccggaccc gactacatga tccccttcgg gaaggcgcgc
     6181 gtcgcgcgcg aggggaagga cgtctcggtc atcacctggg gcgccacggt caaccgggcg
     6241 atgcaggccg cgcagaagct cgaggtcgaa gggatctccg tggaggtggt cgatctcagg
     6301 acgctgctcc ccttcgaccg ggaggcgatc gcggccaccg tccggaagac ggcccgcgcg
     6361 ctggtgctgc acgaggacgt gctgacggga ggcttcggag gagagatcgc cgcggtgatc
     6421 gcccaggacc acttcgagtc gctggacgct ccggtgcgcc gcgtcggcgc cgccgacacg
     6481 ccggtcggct acgcgcccgt tctggaagac gcgatcctgc cccaggtgga tgggatcact
     6541 cgggcgatcc gggagctggc ccggtactga agcggcgagg cggcggtgcc gccacgagtc
     6601 gaaccctcta cgcggcccct tccagaaggt acagctccgt catgatgacg acgtaggtgt
     6661 aggcgtagcc gtcgccgtcg cgagcctgcc gcacgcccat caccccctcg atcccggtcg
     6721 gatcgggagg ctgcgcgacg aggtggggtt cgcgctgccc tgtcttcaca tcgatccggt
     6781 agacctgcac cggcggcgga cccggcaaga acgcatagac ctgccggctg tcggctcccc
     6841 atcccatcac gcgctcccct cgggccatgc cggggatcac gacgggatcg cccccggccg
     6901 cgggatggag ccgcacgatg aaatcctgcc cgctcatcgc gatccaggag ccgtcggggg
     6961 agacctgggg gatccccgcg cccacgtcgg tctccgagaa aagcctgcgc tcgccggtga
     7021 cgaggtcgac aagatacggt ctcatgctcc cctgcgcgtc ggccgcgatg atcgccgcgc
     7081 gggatccgtc gggaaagaag gcgccggcat ggaacatgag gtccccgagc gaaatcgtgc
     7141 gcggctcgcc gatccccgtg ggaatcaccg tgaagcagtg cgggtcggcg atgttgacgc
     7201 tgaggaccca cgtgccgtcg ggcgtgatgt cctgagcgct tccctcgccg atcgcgatcg
     7261 cgggggatcc gtcggttccg cggatgcaga ccaggcggtg ctgcccgcct ccctcgccgc
     7321 tctcgtcgaa gaggacggtg ctcccgtcgg ccgtgatcgc gcgcatcagg gaccagtcgt
     7381 accagctgag atcccgctcc cgatcctcgc cgggcgcctt ggcgatgatg ccggcgcgct
     7441 cgtgtccgtg gaccacgagg acgtcgcccc gcggcgacag gtcctggagc gtgagcgagc
     7501 cggggacccg gagaatggtc cggctcgagc cgtcgaggga gaagcgcatc aagctgcggt
     7561 tcgcacccat cggcgccgcc gtgcaccaga gactctttcc gtcccggccc caggcgagtc
     7621 cctgggccga ggcgaagtcg gtggagatca cgcgaatctc cttcccgcgc tcggcgatgc
     7681 agatccgccc cgcgtcgtcg gctcggtagt cgtggtcgat gaaggccacg cgcgcgccgt
     7741 cgggagagac ccgggggctg ccgatccatc ccaacgtctc gtgcagcacc gtgcccaccg
     7801 ggtactcgag aatcgccttc ccttccttgt gccgcacgat cgcgagcgtc cgtccgtccg
     7861 gggcccagtc cgcggaagca acgttgtcga ggagcggccg tggcgtgcct cccgccatcg
     7921 ggagccgcgc cagcgtgccg tcgtagatgt agccgccata gaagcgccga ttcaggacga
     7981 gcgccatctc tcccgtggcg gagatggcct ggacgtcggc ccgctccaga cgcagcggcc
     8041 gtgaatcggg attcgacgtg tgcacccaga agagctcctt tcccccgccc tcccaggccg
     8101 cggtgtacac gaccgagttc ccgtccgaga cgaatcgcgc gctggacacg atcccgcggc
     8161 gaaaggtgag ccggcggagg gaatggatgg cgggctcctc ggacgcggcc gcggagcccg
     8221 ctcgcatgtc ctcggcaagg tgctcgagga agaaggcgag atccgcggcg caatggaacc
     8281 gatcggcggg gcgcttctcc agacatttgc ggatgaggcg ggccaacccc ggcatctgcg
     8341 cggagacgtc ctcgggcagg gcgggcgcct cctgcgtgag gatggccgcc gcagtctcgg
     8401 tcggagtctc gcgcgcgaag gggtggctgc cggtgatcag ctcgtagagc atcgcaccca
     8461 gcgcgaacag atcggtgcgc gcgtcgaccg cctcgccgcg gagctgctcg ggggccatgt
     8521 agcgggtggt tccgatgagc gcgccggcct cggtcccctc ggtggcccgg gtccgcgtca
     8581 cgccgggccc gtccgtgcca tcgcgaacga gcttggcgat cccgaagtcg aggatcttgg
     8641 cgcggccctc cgacgtcagg atcacgttct cgggcttcag atcccggtgg acgatccccc
     8701 gcgcgtgggc cgcttcgagt ccacgggcga tctgggcgat gcagtcgatc gcctggcgca
     8761 ccgtgaaccc ggagcccgcg atccgctcgc gcaggcttcg gccgtcgagg aactcggaga
     8821 cgagataggg agtgccttcg gattcgccga cgtcgtagac ggtcacgacg ttggggtgat
     8881 tgagcgtccc cgccgcacgg acctcctgct cgaaccggcg cagacgctca ggctcgagcg
     8941 cgacgcccga gccgatcgtc ttcacggcga cgtcgcgccc cagccgcgga tcgcgcgcgc
     9001 gatagacctc ccccatgccg ccggccccta cgaaggccac gacctggtag ggaccgacgt
     9061 tcgttcccaa cggaaaccgc atgagaaagg acttccctct tgctgtggcc tacaggcgca
     9121 ccatcgcgct ctgctcgctg ctcgccgggt cgatgatcgc ctttacctct tcgatccggt
     9181 tcgccgggaa tcctccacgc ttggcgtgct cgcggaccat ctcggcgttc tccgcgtggt
     9241 acacgcagta gatcttgtcc gccgtgacgt agctgtggat ccactggatc ttcggaccca
     9301 tgtcgctcag cacgccgcag gacttctggg aaatggccat caggtccttg gcggtggcgc
     9361 cgccgatctt ggggatatcg cgctcgatca cgtacttggg catggctccc tcctgggcat
     9421 gggctgtcgg tgcgtccggg gaggaaagtc tacatcatgg agagataggc ccgccaggga
     9481 ccgaccgcct cgcggtactg ccgccccagg gtctgtgcga tctgccggag gtgccccagg
     9541 tcgtgcgccg tccacgtggc cagatgctgc cgcagggtca cggggcccag ctcgggatga
     9601 agcccccggc ggtcgagatc ggcgggcgtc atggccagcc cgcggagcgt cgtcacgttc
     9661 tgggcgcgga gcgcggtgaa ctcttccagg agcgacgcga gcgagcgtcc gcgcgtcgcc
     9721 tcgaacatcg cctcgcggtc gaacggcggg aagggttcgg agtcgccctt ctgaaggatg
     9781 tgcttcacgc gggggatcca gtcggtcttc tccccgtgga gcaggtgccc caccacgtcg
     9841 aacgcgctcc aggtccccgg cccgtcggtc gcacggaccc aggcgtccgg cagcccgctg
     9901 agcagcgcgc ggagcgccgc cggagtccgc tcgaggagcg ggatcgagag ctcgggggtg
     9961 tactcgaggc ccatggccgg agcatagacc tctgcgcgcc gttccgctat cccgtcgcgc
    10021 gaacgaggcc gttctcgcgc gccacgcgtc cctcttcctc cagcttctcg agatgcgcga
    10081 gcaaggtccg cgcggagagc gcgggatcgg ccgtcggcgt gtcgtcgtag acgcggaggc
    10141 ggagatcctc ctcgcccagc ggccccgctt cgagcacgcg caggatcttc ccctcgcgca
    10201 gccgccggtg ctcgatcagg gcgtcgagca tccgcgccac cccgcggttg ggcgcgccgt
    10261 gaccgggaat gagggcggtg atgccgatgc ggcggagccg cgtcagggtg gccaggtagt
    10321 cgcggaggtt tccgtcgggc ggcgcgatga tcacggtgcc gagccccgag accacgtcgc
    10381 cggcgatcag cgtgcgcgat gtctcctcga agaaggccag gtgggaacgc gagtgacccg
    10441 gcgtggccag gacgcagagc cggcgcgggt ggcggccgga gagctcgatc cggtccccct
    10501 cggccagcgc gcgatcgatc gggatccgct cggcgacgtc ggcgtgggcc cacacgggac
    10561 aggcgtaccg cgcgcgcact gcctcgacgc cggcgaagtg gtcgcggtgg cgatgcgtga
    10621 ggaggatggc gaggacccgg ccgcgcgagc cctcggcttc ctcgacgacc tggtacaggg
    10681 cctccagctc atccgggttg tcgctccccg ggtctacgat caccacgtcc gagtccccca
    10741 cgaccacggc gttcgtgtgc gtggccggcg gtagggtctc ggtcttcagc ggaatcatcg
    10801 tgatcgcggg gcgcacgtgg acgtagcggg agggaacgcc gttcgcctcg ggaatgtcgt
    10861 gcagccgtcc gggcagcccg tggcctccct gggcgagccg ctggatgacg tagagcgtgg
    10921 gcatcgcgag cacgatgcgg tcctcgtccc acatcttgag cgctaccgcg ggatccaccc
    10981 acgcgcccga ttccagctcg ccaggccaga cgtcgggctc gctcggctcc ggcatgtcga
    11041 tcaggaagaa gttggtgttg aagcgcgcgc ggctgaagtg cggcgtgatc caccgcccgc
    11101 acggcacgta ccgcgccggg tcgaagcgga gtccccaccg gcgctcgacc gtctcccaga
    11161 aggccgactt gccgtcgagc acgtcccgcc ggtggcgccg ctgctcctcc agctggggcg
    11221 ccgacccgtg gaatcctagg aggagccccg tctcctcgaa cgcctcgcgc gcggcgcagc
    11281 gcgccatggt gcgcagattt tcgtccggat cctcgcccag gggtccgtcc tcgtgcgcgt
    11341 accggccgcc cgggaaggaa tggaagcccc cgagaaacgg attcgcctcg gagcggcgca
    11401 cccataggag cttcagccct acctcgcgcg agatcaggat cgtgctcgcc gcggggcgga
    11461 gcggcggcgc ggccgagggg gcgctctcgg gcgccggcgt cgcgctcatt cgacgagcac
    11521 cagggggagc gactcgcgca aggcgcggat gtgctccggc gaggtgccgc agcacccgcc
    11581 gagcacctgc gctccgaggc gcatccagtc cttcgcgagg gccgcgaagc gctcgggagt
    11641 gacggacggg tcgaagctcc aggcgccggt gggtcccacc acgccggcgt tgggatagcc
    11701 gccgaagggg agatgggtgg cgcggctcat gacgtcgagg caggcggcgg tctcgtcgag
    11761 cggcgtgcag ttcacgagca cggccgcggg caaaagcggc tcgaccgcgc ggacggcgtc
    11821 ggcgagcggc tcgcccgaga agagccgggc gccgccgcgg cagacgaagc tcaccgtgaa
    11881 cgggagcccg gtcgcgcggg cggcgcgcgc ggcggccgtc gcctcgtcga tccggttgaa
    11941 ggtctcgagc aggagaatct cggctccgga gcgcgccagg cgcttcgcct ggtcgtcgtg
    12001 ctcgcgctcc atcgcttcct gggggggcgc gtcttccggc cggtaacagt cctcgagcgg
    12061 ccccagcgag ccgccgatcc agacggtccg tcccgcggca ccgcgatcgc gcccctcagc
    12121 cgccagtcga agcgcgatct cggtcaagcg gtccgcgtcc cccgccggcc ggcccacttt
    12181 cgccatcacc cgcggcgtgg tccggaacgt gtcggccgcg agcacgtcgg cgccggcgcg
    12241 cgcgtagtcc tcgtgcacct ggcgcagcgt gtcgggcgcg tccaggatcg cctgcgcgct
    12301 ccagagcgga agcggcgtgg cgacgccgcg gcgctccagc tcggtgccga gtgcgccgtc
    12361 cagaaggacc agctcgccgc gctcgaggcg ctgatggatc gggtgcggag ccatgcgaga
    12421 acgcgcgggt gcgggggtgc tgctccgctc agcccccggc gagcgcctcg tcggcgggat
    12481 tcaagccgtg gaactgcatc agctcctgga tcacggcgcg gcgtctcttg agaatggacg
    12541 tcatcgaggt atagccgtag cggaggatcg cttcccgctc ctggacgctc atggtgttgt
    12601 gcaggaagag gatcacctcg ctggaggagg gctcgatccg atacatcttc acctccggaa
    12661 actcggcctg gtagcggcgg atctcgccgt ccagacgcac tctcgtttcg atgcgcgaag
    12721 cctggtcgtg gatgaaggac attcccttct ccttgatccg cgcgcacttt ccgtcccgcc
    12781 gggggatgca gaccgcctcc tggtcgttcg agatgttgac cagggggttg atgatgagga
    12841 tggtcgaggc tccggccttg atggccacgt ccaggtgcgc caccttcccc gtcgatccgt
    12901 cgatgtagtc gcgcccttcc accttgaagg ggcggaagaa gagcgggatc gccgaggaag
    12961 cggcgacggc gtgcgagagc gggacgttct cggcgtcgac ggcgccgaag acgcgccggg
    13021 ttcccaggtc gagatcggtc gccgggatgt agagctcacg ctcgatgtcg gcgaagctgt
    13081 cggtcttgcc gtgcgcttcc agcaccttcg cgaagaaccg ctggtagttg tccagcgcga
    13141 agagccccgc cggcaggcgc tcctccacgt agtgcatgaa ctcgaacggc gaggggcgca
    13201 cccgctggcg caggcactcc acgaagtagc ccggcatgga cagccccatc ttgatccccg
    13261 agaacaggat cgcccaccgg ttcacgtcgt acatgtcgcc gcgcttgaag tggagcgacg
    13321 aatcggagtt ggtgaggagc gcccagtagg tcttccggat cggaatccct ttggcgagga
    13381 gaccggccac ggtagccccc gcgctcaccc cgatgtagat gtcgaagccg gtgctggaga
    13441 aatcgaagcc gaggaagtcg tcgagggcgg ccaggcagcc gatctcgtag taggccccgg
    13501 ccaggccgcc gccggagagg ctgagcgcga tgcgcttccg gcgcgtggcc gccgtggcac
    13561 cggaagtcga tcgccgccag gcggccctgg cttcggccga gcttccgaaa tccggatggg
    13621 agttggtcat cgttccctat caggataacg gaagagcgcg ttcatcacgc ggtccgaaat
    13681 cccggggaac atgagattca cccaggcgaa cgcccgattc cacgagggga cgtagtggtc
    13741 gggaggattc cggtggagcg cgtgaacgag ggccgcggcc acctgctcgg ggcgcaggat
    13801 cgtgcccgcg ggccccgagc ccagggcgcg ctcgcgcccg gcgcgatcca cgaacggggt
    13861 ctcgaccgcg gccggaagga gcagcgtgac gcggaccccg cgcgggccaa gctcccgccg
    13921 ccaggattcc gtgagcccgg agatcgcgaa cttggtggcg ctgtagtagc cgtagtggac
    13981 cggcgcgacc cgcccggcca tcgagccgat gttcacgatg gcggcgccgg ggcggccgag
    14041 cagcgtcggc agcgccgcgc gcgtcatgcg cacggtcccg aggagattca cctcgaccat
    14101 ctggccgatc tcggcgtcgg tggcctcggt gaggccgcgg agatagccga tgcccgcgat
    14161 gttgaccagg gcgtccagcg cgccgaagcg cgccgtggtc gcggcgacgg cgcgggcgca
    14221 gtcttcagcg cgggtcacgt cgccggagac ggcgagggct tcccctccgg ccgcggccac
    14281 ctcggccgcc agcgcggcga gcggagcctc gctccgggcg aagagcgcca cgcgcgcgcc
    14341 cgccgcggcc agccggagcg ccgccggcct gccgatgccg ccgctcgccc cggtgaggag
    14401 gaccgtcctc cccttaagct ccacgcgtac gcttcatcgt gaccccgccc ctcccgtcca
    14461 ccgcctaccc ggcggcgttc tgccgcgcgc ccgccatcgc cggctcggcc gacgtggcga
    14521 cgccgctgac cggtgccggc gcgccctccg gcgcgagata ccggacgaac cagtcgcgca
    14581 tgaggtgcat gcgcgccacg cggcgcgacg gcgtaccctt ccgcgagagg ccgtggctct
    14641 ctcccgggaa gagcaccatc tccacctcgc gccggagcgc cttcagcgcc ttgtacagca
    14701 tctccgcctc ggtgacgtcg cagcgctggt ccatgaggct gtgcgtgatg aggagcggcg
    14761 tgcggatctt ctcgacgtag gtgagcggcg actggcggcg caggatctcg gcgtcctccc
    14821 acggctcggc gccgaactcg ggcgtggcga ccgggcagag atcgtcgccc aggaggagcg
    14881 tggggatggt cgtgacgcac cgctgcgtga tcgcggcccg gaaacgctcc gtatggccga
    14941 ccacccagtt cgtcatgtag ccgccgtagg agccccccgt gacgccgaga cgctccacgt
    15001 cgatgtcggg atgccgcgcg agggcgtcgt ccacgacgag catgagatcg tcgaacgcgg
    15061 gcgccgccca gtcgtgcacg atcgccttgg tgaacgcctc gccgtagccc tgcgagccgc
    15121 gcggattgga gaagagcacg tagaagccgg cgctcgcgaa catctggaac tcgaggaaga
    15181 agcaggcgcc gtactggacg cgcggcccgc cgtggatctc gaggatcatc gggtgcttcg
    15241 cggtcttctt cgcggccgcg ctcggcggaa tcagccagga ctgcacgcgg tgcccgcccg
    15301 gggcctcggt ccagcgctcc tcgggcgccg agagcgcgag cgaatcgaag agctcgcgat
    15361 tgggcgtgac caggtcggtg aagcggccgt tcgcgggatg gaagcgcgcg acggtgccgg
    15421 cgtcctggtg atcgcagcgg atcacgatca tctcttcggc gcggcggccg cgcttcatat
    15481 cgaggatctg gaccccaccc tgggtcagcg tctccggggt cccgccccgg gcgggaactc
    15541 gatagatgtg ggtcgagccg aactcgctcc cctggaaata gagggtcgat ccgtccgggg
    15601 accagaccgg gtttccgggg gcgtggaagt cgcgcaggtc gcttccgacg agatcggagg
    15661 cgtagcggtc ccagcccgcg gtcaggtcgc gcggggcgcc gcccgcggcg gggacggccc
    15721 agaggtggta gttcgggatg ccccaccagt cgtcgggctg atcgtgtccg aggaacgcga
    15781 tcgtcgagcc gtcgggcgac caggagagcc cgcccgccac gcccggcccg ggcgtgagat
    15841 tgcgcggctc cccgcccgag gcgggcacca cgaacacgtc gctcacgacg taggaggagt
    15901 actcctcgtc ctcctggcgg ttggcggtga aggcgatctg cttcccgtcc ggcgaccagg
    15961 caccggggcc gtgatcgaag ttccccgagg tgagcgcgcg cacggcacgc gtcgcgacgt
    16021 ccagcacgtg caggtgcgag cgcgagcggg gaagccaccc cgagccgtcc agcttgtaga
    16081 gcatgcgcgt gatgtgtcgg aaggtgggct cgggaagctc cttcttgtcc gctttcgcct
    16141 cgcgcgcttt cttctccggc gccgagccgg gcaggtgccc cagctccggc gggtcgctcg
    16201 cgcggaagac gaagaggatc gacgcgccgt cgggcgacca ctcgagcgag ctgggctcgc
    16261 cgtcgaggtc ggtgagccgc tcggcctcgc cgccgtccag cggcaggcgg tagatctgcg
    16321 gcttcttgtc gcggcgggtc gagacgaacg cgatcgtcgt cccgtccggg tcccagaccg
    16381 ccccgaagtt cttgtgctct ccctgcgtca gccggcgcgg cgcgccgcct cccgtcggga
    16441 tcagccacag gtgcgactgg tactcgtcct tctcccggtc gatcgtcttg atctcgtaca
    16501 cggcgcgcgt tccgtcgggc gagggcgaga ccgcgtcgag gaactggatg tcgaacaggg
    16561 tttcgggggt cacggggcgc ttcggcatga atggctccta tttctgcccg gactggaagc
    16621 ggcgcagctg gtcggtcagg tagttgtttc ccggctccat cgcgagggcg cgcgactcca
    16681 cctccaccgc cttgtcggcg tgtcccatcc ggaaatgcac ctcggccagg gtatcgagga
    16741 tgcccgtgtt cttcggctcc aggtcgacgg cgcgttgggc cgcctggagc gcctgatcca
    16801 ggtagacgtc gttggtggcg caggtccagg cgagtccgtt caggttcgac gcgtcgttct
    16861 cgccgcggtc cagggcgcgc tggagcgctt ccccgacgcg ttcgcgctcg atccgggcca
    16921 gctccttctt ccctttcttg tcgtacatct cggcgaagcg aaggtgcgcc tcggccagcc
    16981 gctgcgcctc gaggatgcca aagacccgca ccagctcctt gatcggctcc ttcgcgtcct
    17041 cgacgcgcag gtccacgtag cgctcgcggt actcggggaa ctcgtcgctg gggcggacga
    17101 ccaggatcgc ggcggactgc tggccgcgct tgtcgccccc ggcggcctgc gccacggcga
    17161 ggcagtcgat gaggcgccct gcgagcggct ggcctgccgt ctcggcgaac gtccgtgcga
    17221 gcgcggcgac cgtctcctcg cc
//
LOCUS       DASSLZ010000185         6064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_573273, whole genome shotgun sequence.
ACCESSION   DASSLZ010000185 DASSLZ010000000
VERSION     DASSLZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573273"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VFM00_07320"
     CDS             complement(<1..975)
                     /locus_tag="VFM00_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7498094.1"
                     /translation="MSTTSTTRPGGFPGGRQGPGGPVLELRDITKEYGGNRVLKGISL
                     SVGVGEIHALVGENGAGKSTLMNILFGMPVIHETGGYGGDVLLDGAPVRLTTPAEAMA
                     AGLGMVHQEFMLLPGFRVAENIKLNREPTRRNLLSRIAGRGLERLDIAAMRADARRAL
                     DRVGLSIDEWLPVAGLPVGHMQFLEIAREVDKQALRLLIFDEPTAVLTETEAARLLQS
                     MRAFADAGIAVLFITHRLDEVKAVADRMTVLRDGEVVGTPDPHSTSVVRIAELMVGRP
                     AMAVAHRRGPVEGAAVTLALEGLRVAMPGEPVEDVTLSVRDGEIFGIGG"
     gene            complement(953..2131)
                     /locus_tag="VFM00_07325"
     CDS             complement(953..2131)
                     /locus_tag="VFM00_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HET7498095.1"
                     /translation="MPHRSFTPLRTALSLASGLLLFGLVGCQKSGSTPAVSAVPAVAT
                     PQVAIPFKIGIMTGTVSQGEDEFRAGQMVVAKYGADHVKHVTYPDNFMQEQETVIAQL
                     VGLASDPDVKVIVMGQAVPGAIPGLRKIREKRPDIKVAFVTPQDDPAIVNNEVDICVQ
                     PDELARGRTIVGVAKKMGVTDFVHYSFPRHMSQELIARRFDIMKTTCAQNGIKMHFVT
                     APDPMGEGGLNATQQFVMEDVPRELKQYGQKTAFFSTNCGMMDPMIKSVVMNGGYIPE
                     QCCPSPTHGYPTALGIAIPANKAGDMEYISAQNRKIIAEHHMTGHFSTWPVPEVMLSI
                     RAMTDLLVDAQMGKADYKDPATVKKYMESLAAGHAITLSKYDEKKGNSYLFLVDNIYY
                     "
     gene            complement(2157..3101)
                     /locus_tag="VFM00_07330"
     CDS             complement(2157..3101)
                     /locus_tag="VFM00_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HET7498096.1"
                     /translation="MERVHGLHHITAIAGPAQENLDFYAGVLGMRLVKRSVNQDDPGT
                     YHLFYADAAGHPGSDLTFFPWSEMAPPRMGHGLAVEVSLEAPRGSLDWWAERLERYGV
                     AADPIEVRFGQRALPLSDPHGLRVALIEDPAPRDFEAWAESSVPGERQIRGLHGARLW
                     EREAASTAAFLTEAMGLRALATENGWTRYGFADAAGSVDVRETPEAKRGMWGVGSVHH
                     LAWRVPDDERQMAMRSQVEEAGAQATPVIDRFWFRSVYFREPGGVLFELATDGPGFAV
                     DEDPGHLGETLVLPPWLEQARGQIESRLPELTMPRVEA"
     gene            3178..4350
                     /locus_tag="VFM00_07335"
     CDS             3178..4350
                     /locus_tag="VFM00_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7498097.1"
                     /translation="MPFFLLTAAFLEGFAVTLIQGFLPLYLRQSLGERSFAVLSLVLA
                     IPALGTAVASNFWGGLSDVTGRMKPIILVGAAGYVIALLGIPAVRHGLGVVAWIGLAS
                     LLYGTLAPTAKAYATLVLPERREQGITYVLLAYSSGWLAGSFGGGGLVEGGLGPGLRA
                     AMWTCAGLTGLNALLASRFLPDMHRPRAPAPERRGWAAGVAADLAALYGNPRLFQLCV
                     IAFFCVAGNYLMWGFFTVFLVERLHASLHVVRYALAISSVLGVAGLPFIPPLVRRFGG
                     ARMLAVGITLYLLMYSAMGVTRDPIVAAILYAAPLYGLVHVSANTLAAEVASVSQRGG
                     GLGVLQGAYSIATIVGPLTGGLIADRKGLASIPWVAFAFILAACPLAWLATRRNAR"
     gene            4561..5025
                     /locus_tag="VFM00_07340"
     CDS             4561..5025
                     /locus_tag="VFM00_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HET7498098.1"
                     /translation="MADKPGESKAVKKLEWLAFDAAADKAARQNKHLIVDVYTTWCGW
                     CRVMERETYGNPEVANYLTDNFVLAKVNGESPNKLHWHGEELSERQFARKVGVTGYPA
                     TYFMKPDAEMLGGVPGFIRPPDFMVYSRYVATRWYQRGGIKDYVDSLRTAAQ"
     gene            5179..5997
                     /locus_tag="VFM00_07345"
     CDS             5179..5997
                     /locus_tag="VFM00_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498099.1"
                     /translation="MRSHRIRMPLAAVACAGVLFLAGCSGNNPVNPNGFTLADADDIA
                     AQAGVAAAAGVVTNMSAADGASGGVSGSAAPRFTPLRAGSFAQAETTFTVGAITYTLG
                     VTFYDADGNALSGYGPLAHRMLVTTRATGAVASQQFNASLGHAGSLDVSGLEASLDTL
                     VMNGACSDSVDASFTSLDGTRTRYFIWRSGAVLTDAALLKNRQANPYPLSGTLSYVVH
                     AVRYRSNQFAEVEKTWDATVVIVFDGTATPELTVNGAFHYRLNLVTGAVVRAVA"
BASE COUNT          861 a         2156 c         2028 g         1019 t
ORIGIN      
        1 tccgccgatt ccaaagatct ctccgtcgcg aaccgagagc gtgacgtcct cgaccggctc
       61 gccgggcatc gcgacgcgca gcccctccag ggcgagcgtc acggccgcgc cctcgacggg
      121 accccgccgg tgtgccacgg ccatcgcggg gcggcccacc atcagctccg cgatccgcac
      181 gaccgacgtc gaatgcggat cgggcgttcc gaccacctcg ccgtcgcgca gcaccgtcat
      241 ccggtccgcc accgccttca cctcgtccag acggtgcgtg atgaagagca cggcgatccc
      301 cgcgtcggcg aaggcgcgca tcgattggag cagacgcgcg gcctcggtct cggtcagcac
      361 ggcggtcggc tcgtcgaaga tcaggaggcg gagcgcctgc ttgtccacct cgcgcgcgat
      421 ctcgaggaac tgcatgtgtc cgaccggaag gccggcgaca ggcagccact cgtcgatcga
      481 aaggccgacc cggtccagcg cgcgccgcgc gtcggcgcgc atcgccgcga tgtccagccg
      541 ttccaggccg cgccccgcga tgcgcgagag gaggttcctg cgcgtgggct cccggttcag
      601 cttgatgttc tcggccacgc ggaaccccgg gaggagcatg aactcctggt gcaccatccc
      661 cagtcccgcc gccatcgcct cggcgggcgt ggtcaagcga acgggggcgc cgtccagaag
      721 aacgtcgccc ccgtagccgc cggtctcgtg gatcaccggc atgccgaaca ggatgttcat
      781 cagggtggac ttgcccgcgc cgttttcgcc gaccagcgcg tggatctccc cgaccccgac
      841 cgacagggag atccccttca ggacccggtt tcccccgtac tccttggtga tgtcgcggag
      901 ctcgaggacc ggtccgcccg gcccctggcg gccgccgggg aagccgccgg gcctagtagt
      961 agatgttgtc gacaaggaag aggtaggagt tgcccttctt ctcgtcgtac ttggacagcg
     1021 tgatcgcgtg tcccgcggcc aggctctcca tgtacttctt caccgtcgcg gggtccttgt
     1081 agtccgcctt gcccatctgg gcgtcgacca ggagatcggt catggcgcgg atcgagagca
     1141 ttacttcggg aacgggccac gtggagaagt ggccggtcat gtggtgctcg gcgatgatct
     1201 tccggttctg ggcgctgatg tactccatgt cccccgcctt gttcgcgggg atcgcgattc
     1261 cgagcgccgt ggggtagccg tgcgtcgggc tggggcagca ctgctcgggg atgtagccgc
     1321 cgttcatgac gaccgacttg atcatcgggt ccatcatgcc gcagttcgtg gagaagaacg
     1381 ccgtcttttg cccgtactgc ttcagctcgc ggggaacgtc ctccatcacg aactgctggg
     1441 tggcgttcag gcccccttcg cccatcgggt cgggcgccgt gacgaagtgc atcttgatgc
     1501 cgttctgggc gcaggtcgtc ttcatgatgt cgaagcggcg cgcgatcagc tcctgcgaca
     1561 tgtggcgggg gaaggagtag tggacgaagt cggtcacgcc catcttcttc gcgacgccga
     1621 cgatggtgcg gccgcgggcc agctcgtcgg gctggacgca gatgtccacc tcgttgttca
     1681 cgatggccgg atcgtcctgc ggcgtgacga acgcgacctt gatgtccggc cgcttctccc
     1741 ggatcttgcg cagccccggg atggcgcccg gcacggcctg gcccatgacg atcaccttca
     1801 cgtcgggatc ggaggccagc cccacgagct gcgcgatgac cgtctcctgc tcctgcatga
     1861 agttgtccgg ataggtcaca tgcttcacat gatcggcgcc gtacttggcc acgaccatct
     1921 gacccgcgcg gaactcgtcc tcaccctggg agacggtgcc cgtcatgatc ccgatcttga
     1981 acggaatggc cacctggggc gtcgcgacgg cgggcacggc cgagacggcc ggcgtgctcc
     2041 cgctcttctg acatccgaca agcccgaaaa gaaggagccc ggaggccagg gagagcgcgg
     2101 tcctcagggg agtgaacgag cggtgcggca tgatggcagc ctccgtgtga tgggacctac
     2161 gcttcgaccc gcggcatcgt gagctccggg agcctggatt cgatctgccc cctggcttgc
     2221 tccagccagg gtgggagcac gagcgtctcg ccgaggtgtc ccgggtcctc gtccacggcg
     2281 aacccgggcc cgtccgtcgc cagctcgaac agcactccgc ccggctcgcg gaagtagacc
     2341 gaccggaacc agaagcggtc gatgaccggc gtcgcctgcg cgccggcctc ttcgacctgg
     2401 gaacgcatgg ccatctgccg ctcgtcgtcg ggtacgcgcc acgccaggtg gtgcacgctg
     2461 ccgacgcccc acatcccgcg cttcgcctcg ggcgtctcgc gcacgtccac cgagccggcc
     2521 gcgtcggcaa agccgtagcg cgtccagccg ttctcggtcg cgagcgcccg aagccccatc
     2581 gcttccgtca ggaacgcggc ggtcgaggcc gcttcgcgct cccagagccg cgcgccgtgg
     2641 aggccgcgga tctgccgctc gccggggacc gagctctccg cccacgcctc gaaatcgcgc
     2701 ggcgcggggt cctcgatgag cgcgacgcgc aggccgtgcg ggtcgctcag gggaagcgcg
     2761 cgctgcccga agcggacttc gatcggatcg gccgcgacgc catagcgctc caggcgctcc
     2821 gcccaccagt ccaggcttcc gcgcggcgcc tccagcgaca cctccaccgc caggccgtgg
     2881 cccatgcgcg ggggcgccat ctccgaccag gggaagaacg tgaggtcgct ccccgggtgt
     2941 cccgcggcat cggcgtagaa gaggtggtag gtgccgggat cgtcctggtt cacgctccgc
     3001 ttcaccaggc gcatcccgag cacgcccgcg tagaagtcga ggttctcctg cgccggcccg
     3061 gcgatggccg tgatgtggtg cagcccatgg acccgctcca tgggttgatg gtgagggcgc
     3121 gcgcggcgcg gcgcaagcgt ttcggagggc cgcgcgtctt cgggtactat gcggccgatg
     3181 cccttcttcc tcctgacggc ggccttcctc gagggcttcg cggtcaccct gatccagggc
     3241 ttcctcccgc tctacctccg gcagtccctg ggggagcgga gcttcgcggt gctgagcctg
     3301 gtgctcgcga tcccggccct cggcaccgcg gtcgcgagca acttctgggg cggcctctcc
     3361 gacgtcacgg ggcgcatgaa gccgatcatc ctggtcggcg cggcgggata cgtcatcgct
     3421 ctcctcggaa tccccgcggt ccgccatggg ctgggcgtcg tcgcctggat cggcctcgcg
     3481 tcgctcctct acgggacgct cgcgcccacg gccaaggcgt acgcgaccct cgtcctcccg
     3541 gagcgccggg agcagggaat cacctacgtg ctcctcgcct attccagcgg ctggctcgcc
     3601 gggtccttcg gcggcggcgg cctggtcgag ggcggcctcg gtccggggct tcgggcggcg
     3661 atgtggacct gcgccggcct caccggcttg aacgcgctgc ttgcgtcccg tttccttccc
     3721 gacatgcacc gtcctcgggc gcccgcgccc gagcggcgcg gatgggccgc gggcgtggcc
     3781 gccgacctcg cagcgctcta cggcaatccg cgcctcttcc agctctgcgt catcgcgttc
     3841 ttctgcgtgg cggggaacta cctcatgtgg ggcttcttca cggtgttcct ggtcgagcgc
     3901 ctccacgctt cgctccacgt ggtccgctac gcgctggcca tttccagcgt gctcggcgtg
     3961 gccgggctcc cgttcattcc gcccctcgtg cggcgcttcg gcggcgcgcg catgctggcc
     4021 gtggggatca cgctctacct gctcatgtat tcggcgatgg gggtgacccg ggatccgatc
     4081 gtggcggcga tcctctacgc ggcgccgctc tacggcctgg tgcacgtgag cgcgaacaca
     4141 ctggccgccg aggtcgcgag cgtgtcgcag cggggcggcg gcctcggggt gctgcagggc
     4201 gcctactcca tcgccacgat cgtggggccg ctgacgggag gtctgatcgc ggaccggaag
     4261 gggctggcct cgatcccctg ggtcgccttc gccttcattc tggccgcctg ccccctcgca
     4321 tggctcgcaa cgcgaagaaa tgcgcgttga tccggtgtaa gagcgggtgc tatgcttttc
     4381 cttcggctgg tcggagcggc ccggctgtag cggccgaacc ggccaacacc cccaggagat
     4441 aaggacttag catcgatgct tccgcgccgc gcggtgatcg ccgcgctcac cctggctctt
     4501 ctcgcatttc ccctcgcggg ctccgcccgt accgaacttc cccagccccc aaaagctccc
     4561 gtggccgaca agcccgggga gtcgaaggcc gtgaagaagc tggaatggct ggcgttcgac
     4621 gccgccgccg acaaggcggc ccggcagaac aagcacctga tcgtcgacgt ctacaccacc
     4681 tggtgcggat ggtgccgcgt gatggagcgg gagacctacg ggaacccgga agtcgcgaac
     4741 tatctcaccg acaatttcgt cctcgccaag gtgaacggcg aatcgccgaa caagctccac
     4801 tggcacggtg aggagctctc cgagcgccag ttcgcgcgca aggtcggcgt gaccggatat
     4861 ccggccacct acttcatgaa gcctgacgcc gagatgctcg gcggcgttcc cggctttatc
     4921 cgccctcccg acttcatggt gtactcccgc tacgtcgcga cccgctggta tcagaggggc
     4981 gggatcaagg attacgtgga ttcgctgcgc accgcggcgc agtagaccgg cctccgacac
     5041 acccccgatc catcgcaaat tgcacggccc gccgccgggc cgatgcggcc ggacctcgtg
     5101 ttccggcccg gcacgtcgct tgcctcatcc aggcgggttc cccgccatga ctccgtcgtt
     5161 caacgcagga ggttctcgat gaggtctcac cgcatccgga tgccgctcgc agccgtcgcg
     5221 tgcgccggcg tcctgttcct cgccggctgc tccggcaaca accccgtgaa tccgaacgga
     5281 ttcaccctgg ctgacgccga cgacatcgcc gcccaggcgg gagtggccgc cgcggccggc
     5341 gtggtgacca acatgagcgc ggccgacggc gcctcgggcg gcgtctcggg gtccgccgca
     5401 ccgcggttca cgcccctgcg agccggctcg ttcgcgcagg cggaaacgac gttcacggtg
     5461 ggagccatca cgtacacgct cggcgtcacg ttctacgacg ccgacgggaa cgcgctctcc
     5521 ggctacggtc cgctcgcgca ccgcatgctg gtcaccacgc gcgcgaccgg cgcggtcgca
     5581 agccagcagt tcaacgcttc gctggggcac gcgggatcgc tggacgtgag cggccttgaa
     5641 gcctccctgg ataccctggt catgaacggc gcgtgcagcg actcggtgga cgcttcgttc
     5701 acgtcgctgg atggcacgcg gacgcggtac ttcatctggc ggagcggggc ggtgctcacc
     5761 gacgcggcgc tgctcaagaa ccgccaggcg aacccctatc cgctgagcgg cacgctctcc
     5821 tacgtggtcc atgccgtccg ctaccggtcg aaccagttcg cggaggtcga gaagacctgg
     5881 gatgccaccg tcgtcatcgt cttcgacggc accgcgacgc cggagctgac cgtgaatggc
     5941 gcgttccatt atcggctgaa cctcgtgacc ggtgcggtgg tgcgcgcggt cgcctgaccc
     6001 tcaagcaaaa cacgaaacgg ccggcagagc acttcgctcc agccggccgt ctgtgtttga
     6061 gccc
//
LOCUS       DASSLZ010000186         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_447457, whole genome shotgun sequence.
ACCESSION   DASSLZ010000186 DASSLZ010000000
VERSION     DASSLZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447457"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..761
                     /locus_tag="VFM00_07350"
     CDS             300..761
                     /locus_tag="VFM00_07350"
                     /inference="COORDINATES: protein motif:HMM:NF019238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HET7498100.1"
                     /translation="MRTLGNPADTSSLLHRLHNLRPDSRRRWGRMRVHQMICHLADST
                     RFALGQLTVSAHTPWWARTILKWVALAIPVPWPPGIPTIPELDQVRGAGTKPTDFARD
                     VTELQALVERMAAPDADIEGRVHPLFGRLSRAEWLRWGYLHVDHHLRQFGA"
     gene            complement(772..>2262)
                     /locus_tag="VFM00_07355"
     CDS             complement(772..>2262)
                     /locus_tag="VFM00_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498101.1"
                     /translation="ADAMAEELRQVDAAAGDETPRLARTLMRLVVLPFRVLRPDPETD
                     FLAFSLPDAIATSLTAFSSLVVRSTTTAARFATDAPDLKDIATAAGVDRVVMGTLFRS
                     GEQLRVTVQLVEAPGGTLLTSHAVISSLGDLFLLQDSIARRVVDALALPLHGQAPTPD
                     APQDARAYSLYLQANAIAGDIQKLPEALKLYERCVALDPRFAPAWAQLGRAYRVVGKF
                     LGGPEESEERADEALRRALELNPRLSVAHKYYAALEADDGRSLAALSRLLDEANRHGN
                     DPELFAGLVHAFRYCGLYEQSVLAHLEARRLDPNIVTSLPGTLMMDGTMERDLAAGRW
                     VDVERGQEVTRVMILGIVGRRDEALRELRAIPRENQVPVFHVYADFIRAWLERRPAEM
                     ADWRARVQGLKVLEDPEAMFQEGLLFCDAGDPGIGLDLLRRSVAKGYFPPERLARGAA
                     FDAVRDTPEFQKILADAEAGRAQALEVFRQRGGERLLGHVAAPVAA"
BASE COUNT          387 a          751 c          774 g          350 t
ORIGIN      
        1 gtcaatccat tgcagcagca caggcttaag cagggagtca ttggttcgag tccaacacgg
       61 cccaccatct gtgtcctgag accgcgtagc tcccgacatc cgggtcgaga tggatcccga
      121 atcctggcgt gcaggaaagg acccgcagct ggaggcggcg gttcgagctg cgctcgcagc
      181 tttgagatcg ttcaagaagc ggccctggaa gcgtccgccg tatcccgttc acccgtagcg
      241 gtcgagcggc cccatgctac tgtgctgtga tgacgcagcg ccgcatggtt cgcttgcaaa
      301 tgcgaaccct cggcaaccct gccgacacgt cctcgctgct ccatcgtctc cacaatctcc
      361 gcccagacag tcgccggcgt tggggccgca tgagagtgca tcagatgatt tgccatcttg
      421 ccgactcgac gcgcttcgcg ctgggccagc tcacggtcag cgcccatacg ccgtggtggg
      481 cgcggacgat cctcaagtgg gtcgcactgg cgataccggt gccttggccc cctggaatcc
      541 cgacaatacc tgagctggat caggtgcgtg gggcgggaac gaagccgacg gatttcgcgc
      601 gggacgtgac ggagctgcaa gcgctggtgg agcgtatggc cgcgcccgat gccgatatcg
      661 aggggcgcgt gcaccctcta ttcggacgcc tctccagagc ggagtggttg cggtggggat
      721 acctccacgt ggatcaccac ctgagacagt tcggagccta gaggccgggg gctacgccgc
      781 gaccggtgcg gcgacgtgac cgagaagacg ctcgccgccg cgctggcgaa agacctccag
      841 cgcctgcgcc cgtcccgctt cggcgtccgc gaggatcttc tggaactcgg gggtgtcgcg
      901 cacggcatcg aacgccgcgc ccctcgccag ccgctccggc gggaagtacc ccttggcgac
      961 cgagcgtcga agaagatcga gcccgatccc gggatctccc gcatcgcaga acaggagccc
     1021 ctcctggaac atcgcttcgg ggtcctccag aaccttcagc ccttgcaccc tggcgcgcca
     1081 gtccgccatc tcggcgggtc gccgctcgag ccacgctcgg atgaaatcgg cgtacacgtg
     1141 gaagaccggg acctggtttt ccctggggat cgcgcgcagc tcccggagcg cctcgtcccg
     1201 gcggcccacg atcccaagaa tcatcacgcg cgtcacctct tgtccgcgtt ccacgtcaac
     1261 ccaacggccc gcggcaaggt cgcgctccat cgtgccgtcc atcatgagcg tgcccgggag
     1321 gctcgtcaca atgttcgggt cgagccgccg cgcctcgagg tgcgcgagca ccgactgctc
     1381 gtaaagacca caataacgga aggcatggac caggccggcg aacagctcgg ggtcgttccc
     1441 gtgacggttc gcctcgtcca ggagacgtga gagcgcggcg agggagcggc catcgtccgc
     1501 ctccagggcc gcgtagtact tgtgcgccac cgaaagccgc gggttcagct cgagcgcgcg
     1561 gcgaagcgct tcgtcggcac gctcttcgct ctcttcgggg cctcccagaa acttgccgac
     1621 gacgcgatac gcgcggccga gctgggccca ggccggggcg aagcgcggat ccagcgccac
     1681 gcacctctcg tacagtttca gggcctcagg gagcttctgg atatcgccgg cgatggcatt
     1741 ggcctggagg tagagcgaat acgcgcgggc atcctggggc gcgtccggcg tcggtgcctg
     1801 gccgtggagc ggcaacgcga gcgcgtccac gacgcgccgg gcgatactat cctgtaaaag
     1861 gaagagatcg cccagggagg agatcacggc gtgcgaggtg aggagcgtgc cgcccggagc
     1921 ctcgacgagc tggacggtga cgcggagctg ctcgccggag cggaaaaggg tccccatcac
     1981 cacccgatcc acgccggcgg cggtcgcgat gtccttgaga tcgggcgcat cggtggcgaa
     2041 gcgggccgcg gtcgtggtgg atcgcacgac gagggagctg aacgcggtga gcgacgtcgc
     2101 gatcgcgtcc ggcaggctga acgcgaggaa gtcggtttcc ggatcgggtc tcaagacccg
     2161 gaaaggaaga accacgaggc gcatgagggt ccgcgccagg cggggcgtct cgtcgccagc
     2221 ggcggcgtcg acctgacgaa gctcctcggc catggcgtcg gc
//
LOCUS       DASSLZ010000187         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_67152, whole genome shotgun sequence.
ACCESSION   DASSLZ010000187 DASSLZ010000000
VERSION     DASSLZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67152"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFM00_07360"
     CDS             complement(<1..608)
                     /locus_tag="VFM00_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7498102.1"
                     /translation="MKISTRGRDAQASPIRRLAPYAEEAARQGVKVYQLNIGQPDLPT
                     PEIVMERIRNYPGKYVPYAPSPGIPEFREGMSRYYKSVGLDVSPAEVLTTVGGSEALQ
                     FAFWALFEPGEEVIIFEPFYTNYATMALVAGVGVKPIPCDGRTGYHLPPVEAIERAIG
                     PKTRGILLCAPSNPTGTAYTAAEIDAVVELAMKHDLWIITDEA"
     gene            complement(605..1738)
                     /locus_tag="VFM00_07365"
     CDS             complement(605..1738)
                     /locus_tag="VFM00_07365"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498103.1"
                     /translation="MSPASPRSARATKATTAMDSLLRPLLEAYGPPGREAAVRAVLRR
                     QLRGAGTWEEDATGNLHLHRAGEGPRLLLAAHMDAPGIIVTRLDGETGQARLSILGGR
                     RPAELVGAPLRFEDGRAAIVSWDRKEGADPEMDQLLVETGVGRSASRRATRANGSRPL
                     DVGDVAAIDDRPSRLGDYWCSANLDDRAGCAALVHALTRARRVRYDVHAVFTAQSDLG
                     ARGATTGTFGIDPEMAIVVDVAHLGEPKETSGFSLGKGPCVALKEQGFLAHPEMLERV
                     RKAARAARIPLQYLIREGEGSDARAVRAARSGVPTAVLAIPARRTGGVGSLVHVSDLE
                     QTATLIAALLQGSGGAAGEGSKSRRSQPKARAGARQTKRGGRK"
     gene            complement(1735..2886)
                     /locus_tag="VFM00_07370"
     CDS             complement(1735..2886)
                     /locus_tag="VFM00_07370"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7498104.1"
                     /translation="MSASASRRARGAARGGSRGGAPAFREDLLESLSNAAGVSGDEHR
                     VREIVAAAVRERVDTLQVDAMGSLVATIGLEGPGARNGNARPNGTARKGRPPHVMLCA
                     HMDEVGFMITAIEKEGRLRFRRAGGIDPRILVGQVFRVGSSGVRGVTGILPPHLVSHN
                     EEEKVIAADDLYIDIGASSREDALRHVAIGDTAVFDTTYEAWGSVRKGKAFDDRAGCA
                     VLASLVQERPPVPVTAVWSVQEEVGLRGATAAARRVAPDLAIVLEGTASGEMPGAGPE
                     ESAPRMGGGPTLTVHDRSLMADARLVDLLRRTAAERRIPTQWKRPGVGGTDAGRIALS
                     GAGVPSAVVSVPCRYIHAPAAYLDVRDARRTVELVWHALRAIGKEWSES"
     gene            complement(2883..>3821)
                     /locus_tag="VFM00_07375"
     CDS             complement(2883..>3821)
                     /locus_tag="VFM00_07375"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HET7498105.1"
                     /translation="PIGNLYGWIDGSGQAPRPRAMLAAHMDRIGFLVKRIEKGGFLRV
                     TSVGGFDPRTLPGKEVMVHAKPPMPALFGTKPPHLQARGESEKVVPLHELYLDTGRPE
                     AEVRRRVPIGTMVTLRQPPVDLLNGRIASPGMDDRAGVVVVIRTLELLRDRRPEWDVV
                     GVATIEEEFGTVFLGARTATERVDPKLGIAIDVSHGDMLGAREADTIPLGGGPGLTVG
                     SNVHPRVFEGLRATARSLKIPYHVEAYPASSGTDAMDIQIAREGIPTAVLGIPCRYMH
                     SGVETVEPRDVDACARLLAGYLAGLPADWSVTEVLK"
BASE COUNT          500 a         1408 c         1352 g          561 t
ORIGIN      
        1 gcctcgtcgg tgatgatcca gaggtcgtgc ttcatcgcca gctcgaccac ggcgtcgatc
       61 tcggccgcgg tgtaggcggt gccggtggga ttggagggcg cgcagagcag gatgccgcgc
      121 gtcttgggac cgatcgcccg ctcgatcgct tcgaccggcg gcaggtgata gccggtgcgc
      181 ccgtcgcagg ggatcggctt cacgccgacg cccgccacga gggccatggt cgcgtagttc
      241 gtgtagaacg gctcgaagat gatgacctct tcgcccggct cgaagagcgc ccagaacgcg
      301 aactggagcg cctcgctccc gcctacggtc gtgagcacct cggcgggaga gacgtcgagc
      361 ccgacgctct tgtagtagcg cgacatcccc tcgcggaact cggggatgcc gggcgaaggg
      421 gcgtacggca cgtacttgcc cggatagttc cggatccgct ccatcacgat ctccggcgtc
      481 gggagatcgg gctgcccgat attgagctga tagaccttca cgccctggcg cgccgcctcc
      541 tccgcgtacg gcgcgagccg gcggatgggg gaagcctgcg cgtcgcggcc ccgtgtcgag
      601 atcttcattt ccttcctcct cgtttcgtct gccgagcgcc cgcgcgcgcc ttgggttggc
      661 ttcgcctgct cttcgaccct tcgccggccg cgccgccgct cccctggagg agcgcagcga
      721 tcagcgttgc cgtctgttcc agatcgctca cgtgcaccag cgatccgacg cctcccgtcc
      781 ggcgcgcggg aatcgcgagg accgcggtcg ggacgccgct tcgggccgct cgcaccgcgc
      841 gcgcgtcgct tccctcaccc tcgcggatca ggtactggag cgggatccgc gcggcgcgcg
      901 ccgccttgcg gacgcgctcg agcatctccg ggtgggcgag gaatccctgt tccttgagcg
      961 cgacgcaggg ccccttgccg agcgagaagc cgcttgtctc cttgggctcg ccgaggtgcg
     1021 ccacgtccac cacgatcgcc atctcgggat cgatcccgaa cgtgccggtc gtggcgccgc
     1081 gggcgcccag gtccgattgc gccgtgaaga ccgcatgcac atcgtagcgc acccgccggg
     1141 cgcgcgtgag ggcgtggacc agcgcggcgc agccggcacg gtcgtcgaga ttggcggagc
     1201 accagtagtc cccgaggcgc gaggggcggt cgtcgatcgc ggcgacgtcg cccacgtcga
     1261 gcgggcggct cccgttcgcg cgcgtcgcgc ggcgcgaggc ggaccggccg acgccggtct
     1321 cgaccaggag ctgatccatc tcgggatcgg cgccctcctt ccggtcccag ctcacgatcg
     1381 cggcgcggcc gtcctcgaaa cggagcggcg cgcccaccag ctcggccggc ctccggcccc
     1441 ccaggatcga caggcgcgcc tgtcccgtct cgccgtccag ccgcgtgacg atgatgccgg
     1501 gggcgtccat gtgcgcggcg agcaaaagcc gcggcccctc gccggcgcga tggagatgca
     1561 ggtttccggt cgcgtcctcc tcccaggttc cggcgccgcg caactggcgc cgcaacacgg
     1621 cgcgcaccgc ggcttcccgg cccgggggtc cataggcttc gagaagcggc cggagcaggg
     1681 aatccatagc cgtggtggcc ttggtggccc tggcggatct cggggaagcg gggctcatga
     1741 ttccgaccac tcctttccaa tcgcgcgcag cgcgtgccag acgagctcca cggtgcgccg
     1801 ggcgtcgcgc acgtcgaggt atgccgcggg ggcgtggatg taccggcagg gaaccgacac
     1861 caccgccgag ggaacgccgg cgcccgagag cgcgattctc cccgcgtcgg tgccgccgac
     1921 gccgggccgt ttccactggg tggggatgcg ccgttccgcc gccgtccggc ggagcaggtc
     1981 gacgagccgc gcgtcggcca tgaggctccg gtcgtgcacc gtcagcgtgg gacctccccc
     2041 catgcgcggg gcgctctcct ccgggccggc gccgggcatc tcgccggagg ccgttccttc
     2101 gaggacgatc gccagatcgg gggcgacgcg gcgcgcggcg gcggtggcgc cgcgcaggcc
     2161 gacctcctcc tggaccgacc agaccgccgt gaccggcacc ggcgggcgct cctggaccag
     2221 actcgccagc accgcgcacc ccgcgcgatc gtcgaacgcc tttcccttgc gtaccgagcc
     2281 ccaggcttcg taggtcgtgt cgaacacggc ggtgtcgccg atcgcgacgt ggcgcagcgc
     2341 gtcctcgcgc gaggaggcgc cgatgtcgat gtagaggtcg tcggcggcga tgaccttctc
     2401 ctcctcgttg tgcgacacga ggtggggcgg caggatcccg gtcacgccgc gcacgcccga
     2461 gctgcccacg cggaagacct gtcctacgag gatgcgaggg tcgatcccgc ccgcgcggcg
     2521 gaagcggagc cgtccctcct tctcgatcgc ggtgatcatg aagcccactt catccatgtg
     2581 ggcgcagagc atcacgtggg gcggccgtcc cttgcgggcc gtgccgttgg gccgcgcgtt
     2641 cccgttccgg gcgcccggtc cctcgagccc gatggtcgcg accaggctcc ccatcgcgtc
     2701 cacctggagc gtgtccaccc gctcgcgcac ggcggccgcc acgatctcgc gcacgcgatg
     2761 ctcgtcgccg ctcacgccgg cggcgttgga gagcgattcc agcaggtctt cacggaaggc
     2821 cggggcaccg ccgcgggagc ccccgcgcgc cgcgccccgg gcgcgccgcg aagcgctcgc
     2881 gctcacttga gcacctcggt cacgctccag tccgccggca gcccggcgag gtagccggcc
     2941 aggagccgcg cgcacgcgtc cacgtcgcga ggctccaccg tctccacgcc gctgtgcatg
     3001 tagcggcagg gaatgccgag caccgcggtg ggaattccct cccgcgcgat ctggatgtcc
     3061 atggcgtcgg tgcccgacga cgcggggtag gcctcgacgt gataggggat cttgagcgag
     3121 cgggccgtgg cgcggagccc ctcgaaaacg cgcggatgca cgttcgaccc gacggtgagt
     3181 ccgggcccgc ccccgagggg aatcgtgtcg gcctcccgcg cgcccagcat gtcgccgtgg
     3241 ctcacgtcga tcgcgatccc cagcttcggg tccacgcgct cggtcgcggt gcgcgcgccc
     3301 aggaacacgg tgccgaactc ctcttcgatc gtggccacgc ccaccacgtc ccactccggg
     3361 cggcggtcgc gcaggagctc gagcgtccgg atcacgacga cgacgcccgc gcggtcatcc
     3421 atgccgggag aggcgatcct gccgttcagc agatccaccg gcggctggcg cagcgtgacc
     3481 atggtgccga tcgggacgcg ccgccgtacc tcggcctcgg ggcgtcccgt gtcgaggtag
     3541 agctcgtgca ggggcaccac cttctccgac tcaccccgcg cctggagatg gggcggcttg
     3601 gttccgaaga gcgccggcat gggcggcttc gcgtgcacca tcacttcctt gccggggagc
     3661 gtgcgcggat cgaagccccc caccgacgtg acgcgaagga agcccccctt ctcgatccgc
     3721 ttcacgagga agccgatccg gtccatgtgc gccgcgagca tcgcgcgcgg ccggggggct
     3781 tggcccgaac cgtcgatcca cccgtagagg ttcccgatcg g
//
LOCUS       DASSLZ010000188         3210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1835178, whole genome shotgun sequence.
ACCESSION   DASSLZ010000188 DASSLZ010000000
VERSION     DASSLZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835178"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFM00_07380"
     CDS             complement(<1..621)
                     /locus_tag="VFM00_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498106.1"
                     /translation="MIRFRWISNGLSIGALALAALSVLSPSPARAQSWLWEIPVGLEP
                     GFRQPGYVPGELLVQFKGSVTAAQRKAAAASEGAQVAGDVTPDGLVKIKLAPGQTVED
                     MIDHWSARSDVDYAAPNLYARGFFTPNDTTIAHFDLAWNLRAVHAYDAWDVVTGHPDV
                     VLAIIDTGVAFEDREIPEYERPFVKPGVTMYRRSPELPGPFLPGWDF"
     gene            complement(760..1827)
                     /locus_tag="VFM00_07385"
     CDS             complement(760..1827)
                     /locus_tag="VFM00_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017641306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7498107.1"
                     /translation="MASLTLAALTLTYPNGTRAVDGVDLALDDGAFLAILGPSGCGKS
                     SLLRLIAGLETPTSGSIRMDGEDVTGKEPKDRDVAMVFQGLALYPHMTVAENMGLGLR
                     ARKTPQGETDRRVKEAAETLGLTRYLQKRPRELSGGERQRVAVGRALVRRPRLYLFDE
                     PLSSLDAQLRQELREELARLHRLTRTTSIYVTHDQKEALSLGDQVAVMRAGQVRQIGA
                     PQSVYRDPRDLFVARFVGEPPINLIEGEVVSGAGGAAFQASGWRLELGAAARPRIGRA
                     LLGLRPEGVALSGGDMPARVERVEHLGGESLLHASGDWGAVVIRIPPSEAAPAPGASV
                     RLRPDPSRAVLFDPESGERLA"
     gene            1904..2320
                     /locus_tag="VFM00_07390"
     CDS             1904..2320
                     /locus_tag="VFM00_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498108.1"
                     /translation="MNRFPIAFAQTVAFAAATGSMAWQILRILIHRGAILSPVVIALL
                     IAGAFAAGGLLFALVLIPLRKRLAAHGEPGRYPTPLLLLQIGISTVWALAWTFPGFQL
                     VGRGGTDVFWGLLCFSMGGFTGVLFYLPAGWHRTPV"
     gene            complement(2346..2819)
                     /locus_tag="VFM00_07395"
     CDS             complement(2346..2819)
                     /locus_tag="VFM00_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498109.1"
                     /translation="MQRGYVYRRTEPMGRHFHPDFRPELTPKQLLRLGVFGGRYMTDC
                     REEFPADWFRFAKLCPERHDPSLNYFGVNASQPLSVWRKKGWIYREDPRGWFQWYCRY
                     YSGRRCPDDERQIQRWRAVRRHVAQIEKNCRRGDISCRPRQRQALLHWAYDSRKI"
     gene            complement(2857..>3210)
                     /locus_tag="VFM00_07400"
     CDS             complement(2857..>3210)
                     /locus_tag="VFM00_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498110.1"
                     /translation="AARWPAAKSPAVPEASGYVTIPNAVVPPEKTHIYKAIYDATRGA
                     DKPTQILPALDMAGSELNALAASGIPLRNARCGQNLAFEDRDPRAIAREVKIASDALI
                     VLMKYQNGGYALLSF"
BASE COUNT          458 a         1088 c         1160 g          504 t
ORIGIN      
        1 gaagtcccag ccgggaagga aggggccggg aagctcgggc gagcggcggt acatggtcac
       61 gcccggcttg acgaaggggc gctcatattc gggaatctcg cgatcctcga acgccacgcc
      121 cgtgtcgatg atggcgagca cgacgtcggg gtgtccggtc accacgtccc aggcgtcata
      181 ggcgtgcacc gcgcgcagat tccaggcgag atcgaaatgc gcgatggtcg tgtcgttggg
      241 cgtgaagaag ccccgggcgt acaggttcgg cgccgcgtag tccacgtcgg agcgcgcgct
      301 ccagtggtcg atcatgtcct cgacggtctg gccgggggcg agcttgatct tgacgaggcc
      361 gtccggcgtc acgtcgccgg ccacctgggc gccctcggac gcggccgccg ccttccgctg
      421 cgcggccgtg acggagccct tgaattggac gagcagctcg ccggggacgt atccgggctg
      481 gcgaaatccg ggctccagcc ctaccgggat ctcccagagc cacgattgcg cgcgagcggg
      541 gctcggggag agcacggaga gcgccgcgag cgccagggcg ccgatcgaaa ggccgttcga
      601 aatccaccgg aaccggatca tgcgtcctcc gcggaaggtc ggggcgcgtt gcgggcccgc
      661 tgcggtgtgc gatccggggg gacggcggca ctcgtggcgg tgcgagatgt cctataagtc
      721 tagcatacgc acgaggccgg acggcgggac cggaaaaggc taggcgagcc gttcccccga
      781 ctcgggatcg aagagaacgg cgcgggaggg atcggggcgt agccgtacgg acgcccccgg
      841 cgccggggcg gcttcggacg gggggatccg gatcaccacc gctccccagt ccccgctcgc
      901 gtggagcagc gactcgccgc ccaggtgctc cacccgctcg acacgcgccg gcatgtcccc
      961 gcccgagagc gcgacccctt cggggcgaag gccgagcagg gcgcgcccga tccgcgggcg
     1021 cgcggccgcg cccagctcca ggcgccagcc gctcgcctgg aacgcggcgc cgccggcgcc
     1081 gctcaccacc tcgccctcga tcaggttgat cggcggctct cctacgaagc gcgcgacgaa
     1141 gaggtcgcgc gggtcgcgat agaccgactg cggcgctccg atctgccgga cctgccccgc
     1201 ccgcatgacg gcgacctgat cgcccaagga cagcgcctcc ttctgatcgt gcgtgacgta
     1261 gatcgacgtg gtgcgcgtga gccggtggag gcgcgccagc tcttcgcgga gctcctggcg
     1321 gagctgggcg tccaggctgg agagcggctc gtcgaagagg tagagccggg ggcggcgcac
     1381 cagcgcgcgg cccaccgcga cccgctggcg ctcgccgccg gagagctcgc gggggcgctt
     1441 ctggagatag cgcgtgagcc ccagcgtctc cgcggcctcc ttcacccggc gatcggtctc
     1501 gccctgcggc gtcttccgcg cgcggagtcc cagccccatg ttctcggcga ccgtcatgtg
     1561 cgggtagagc gcgagcccct ggaacaccat cgccacgtcg cgatccttgg gctccttgcc
     1621 cgtgacgtcc tccccgtcca tgcggatcga gccggaggta ggcgtttcga gcccggcgat
     1681 gaggcggagg aggctggatt tgccgcaccc ggagggaccg agaatcgcga ggaacgcgcc
     1741 gtcgtcgagc gcgagatcga cgccgtccac ggcgcgcgtg ccgttgggat aggtcagcgt
     1801 gagcgcggcg agcgtgaggg aagccatgcg cggctacggt agcaggcgat gggggccggt
     1861 gctaggatgt ccgcattccc ccggtctctt cgaggaggct ttcgtgaatc ggttcccgat
     1921 cgcgtttgcc cagaccgtgg cgttcgccgc ggccaccggc tccatggcct ggcagatcct
     1981 ccgtatcctc atccatcgcg gcgccatcct gagtcccgtc gtcatcgcgc tgctcatcgc
     2041 gggggcattc gcggcgggcg ggctgctttt cgccctcgtg ctgattcccc tacggaagcg
     2101 ccttgcggcc catggcgagc cggggcgcta tcccacaccg cttctcctat tgcagatcgg
     2161 gatctccacg gtttgggccc tggcatggac gttcccggga ttccagctcg tcggccgtgg
     2221 cggcacggat gtgttctggg gattgctctg tttttcgatg ggagggttta ccggggtgct
     2281 cttctacctg ccggcaggat ggcaccgcac ccctgtctga tcgcgctcca gcggtcctct
     2341 cacggctaga tcttccgcga gtcgtacgcc cagtggagca gcgcctggcg ctgccgggga
     2401 cggcacgaga tgtccccgcg gcggcagttc ttctcgatct gcgccacgtg ccggcgcacc
     2461 gcgcgccagc gctggatctg ccgctcgtcg tcgggacacc ggcgcccgga gtagtagcgg
     2521 cagtaccact ggaaccagcc gcgcggatcc tcgcggtaga tccatccctt cttccgccac
     2581 accgagagcg gctgcgacgc gttcacgccg aagtaattga gcgaggggtc gtgccgctcg
     2641 ggacagagct tcgcgaaccg gaaccagtcg gcgggaaatt cctcgcggca gtcggtcatg
     2701 tagcgcccgc cgaagacgcc gagccgaaga agctgcttgg gggtcagctc cgggcggaag
     2761 tcgggatgaa agtgccgccc catcggctcg gtgcggcggt agacgtagcc gcgctgcatc
     2821 cggtcgttca cgacgatccg cgcgtcgagg ctcacgtcag aagctgagga gcgcgtagcc
     2881 gccgttctgg tacttcatca ggacgatcag cgcgtcgctc gcgatcttca cctcgcgcgc
     2941 gatcgcgcgc gggtcgcggt cctcgaacgc gagattctgc ccgcaccggg cgttccggag
     3001 cggaatgccg gaggccgcga gcgcattcag ctcggatccc gccatgtcga gcgcggggag
     3061 gatctgcgtc ggcttgtccg cgccgcgcgt agcgtcgtag atcgccttgt agatgtgggt
     3121 cttctccggc ggaacgacgg cgttcgggat cgtgacgtag ccgctcgcct cgggcacggc
     3181 gggggacttg gcggccggcc agcgcgccgc
//
LOCUS       DASSLZ010000189        10022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_70899, whole genome shotgun sequence.
ACCESSION   DASSLZ010000189 DASSLZ010000000
VERSION     DASSLZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70899"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFM00_07405"
     CDS             complement(<1..977)
                     /locus_tag="VFM00_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498111.1"
                     /translation="MNRSAIWTLGVTLLVSLPAISLSGCSSTRSDVAYRDDYEAGYSN
                     YHFSSQPRMRRVGDTDVYYIRDASDYDVYQFEGSWYLNDQGRWYRAGSWRGPWASMDV
                     AVLPYEISTVPSEYRRNWVSVRVDRRDPRYRNLPEGYWASGRTFSTKPAMGVIPNSSV
                     TYARQAADLDLYRLHGTWYLVDGGVWYRSTSWRGPFLSIRASAVPREVLTIPSRYRHD
                     WSVSSTDDDDRDSDRYRDSDRYRDRDRYQTVRYWNSGRTFTAEPNYDMIPGTDIDYVR
                     GNASYDLYRYNDSWYLADQTGWYRADTWRGPFISIQLGSVPRAVISVPMT"
     gene            1200..1760
                     /locus_tag="VFM00_07410"
     CDS             1200..1760
                     /locus_tag="VFM00_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498112.1"
                     /translation="MVPIPSLWLPVLLSAVIVFLASWIAHAVLPHHKNDVKHVPAEDA
                     FLEAVRKLDLPPGDYMAPRPSSMAAMKNPEFVEKRKRGPVVIMTVIPGGPPSMGKELG
                     QWFLFTIVVGIFAAYITGHAVEVGAPYLRVMRFAGATAFFCYSIAQFPESIWYKRSWA
                     TTFKNMFDGLIYGLLTGGVFGWLWPR"
     gene            complement(1865..2131)
                     /locus_tag="VFM00_07415"
     CDS             complement(1865..2131)
                     /locus_tag="VFM00_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7498113.1"
                     /translation="MNILWTIIIGLVIGALAKLIMPGRDGGGILMTIILGIAGSLLAG
                     FIGRSMGLYDRGEGAGIIASIIGAVLLLAIYRAITHNRHRSLPV"
     gene            complement(2413..3012)
                     /locus_tag="VFM00_07420"
     CDS             complement(2413..3012)
                     /locus_tag="VFM00_07420"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET7498114.1"
                     /translation="MKPPASAIAAALLFITAPSAGRAATFLVHPGGENKVSFTSRAAM
                     ERFEGKTSRLEGSIVVDPATVGDSATVHFEVDLASLDTGIAKRNQHMREAHLETATYP
                     KAVFDGVAIRPGSGALSSGKPVTLDVEGTFTLHGVAHRIQLTVVAAYKQSGKEDRIEF
                     QTAFPVALADYRISRPQFLFLKLADVQQVRVRGVAVASP"
     gene            complement(3021..4118)
                     /locus_tag="VFM00_07425"
     CDS             complement(3021..4118)
                     /locus_tag="VFM00_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498115.1"
                     /translation="MIRLLVVGLALLGLGLLAARPGVALPRYSARYQQTCALCHVNPT
                     GGGLRTSYATEKIIPEEIAWRRGGLPALEDVAPSIARHVQVGTDFREVYVGADLPQDH
                     LNFFQMQADLYFSFQLDSTVALYYDRGESGSYELFGLDYLRPELYVKAGRFVPSYGWK
                     FDDHTMFVRAELGFMPPANSDVGLEAGYARGPLDLQAAVVNGNRGLVMDSDTKVAGVV
                     NAVFRHRLGPVGASLGGAAYHQPGFARDYDTWGLYGYLTWNRLTWLAEGDFFQNRPAG
                     GPATIGAVTSHEVTVLVRQGLELKGTYDFFDPDRDKASGAKSRYGGGVAVMPRPYAAC
                     EALVRRTAYEEGVAYSGRDLIETVIMLHLLY"
     gene            complement(4129..5106)
                     /locus_tag="VFM00_07430"
     CDS             complement(4129..5106)
                     /locus_tag="VFM00_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HET7498116.1"
                     /translation="MRPSRHLAIVVIATALACSGCSDLGRPLRPLPQPLLSAETLDFG
                     AVTAADSAFREVTVTNAGTGALLGNAQVSCPEYAIVSGGGPFSIPPGGSLTIRLRFHP
                     AAEGAFSCDLDLGFPSGRVALSGSGALQTPGARWIVLPDSLDFGVLAAGETATRTFTI
                     FSVGTAPLNVNVSGSCGAFQILSGDGAAAIPPGGSVTVSVQFAPTTGRGQSCSVSLGP
                     GIPDVPISGFATTVSFANDVQPILQNCDGCHAFSDPGSGYRILTQDRIFTYPPGVRVI
                     PFDLTNSVLYGKITNSGQYGQLMPQGGPLLPQVDRDTIRDWILEGARNN"
     gene            5306..5812
                     /locus_tag="VFM00_07435"
     CDS             5306..5812
                     /locus_tag="VFM00_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET7498117.1"
                     /translation="MRLLVLATRLLLLSSFLAAGCAVSRPVARLDSTPVSAELAILPT
                     PVPDLGGADISTGPEDAPAPAPRPHRALTRGQVGTASFYDASFNGQTTANGDTYDDAK
                     LTAAHRTLGFGTRVRVTNLENHRSVVVTVNDRGPFVGGRVIDLSRRAARALDFVEDGT
                     TRVRVQPL"
     gene            5829..7016
                     /locus_tag="VFM00_07440"
     CDS             5829..7016
                     /locus_tag="VFM00_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET7498118.1"
                     /translation="MIASRRSTLSKAFALAPAVVMALAASAPSCFALTLHVGPGKSYA
                     TVRAAAEAALPGDVVLIDAGVYSGDVATWKSDNVTVRAVGGRAHMKADGAQEGGKAIW
                     VVQGRNFTAEGIEFSGAKVPDQNGAGIRSETPGTLILRDCYFHDNEEGILGGADSMVI
                     ENCVFDRNGQGDGRTHNIYASGRSFTLRGSTVKRAVIGHNVKTRAKNNYILYNRILDE
                     ASGTASYEVDVPDCGRTYLIGNVIEQGPKSENATIISYGAESASNVQDLYVVNNTIVN
                     DRQDGTFLQIRDGTKALVLNNIFYGPGTPWAGGRVVESRNYVEPSMHNGPGFADPRTF
                     DYRLTASSPPEIVDAGGAVGLSITGFDLTPTGQCGDDARVRARARAGALDIGAFEREP
                     AKP"
     gene            complement(7013..7504)
                     /locus_tag="VFM00_07445"
     CDS             complement(7013..7504)
                     /locus_tag="VFM00_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7498119.1"
                     /translation="MIRAMDKDTPLREQIARLLAWEDAHAGFDAAVGGIAPRHRGAVP
                     QGMPHSPWQLLEHLRLAQRDILEFCTAPKYEEMKWPEDYWPKSPAPESDAAWDRSVAA
                     FHADLEALAALARDPSVDLFGRVPNGTGQTYLRELLVVADHNAYHIGQLVLVRRALGI
                     WGS"
     gene            7607..8428
                     /locus_tag="VFM00_07450"
     CDS             7607..8428
                     /locus_tag="VFM00_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET7498120.1"
                     /translation="MTTSPEQPGAGGDDPLLAYRDPAFLDSEAARPIRIISEYLAPLD
                     AFCRECVSDTIVFFGSARIAPDGPMGRYYAEARELARLITEWSRSLKSPGHRYIVCSG
                     GGPGIMEAANRGAQEAGGRTIGLNIGLPHEQRPNPYITPGLSFEFHYFFMRKLWFAHL
                     ARAMVVFPGGFGTLDETFEMLTLSQTRKLDRSLPVLLYGSSYWKELIQFEALVRHGMI
                     DRSDLDLFVFVDEPREALDILRQRLGEEKKPPAPAFADSTTPESPMPTPPRGPRG"
     gene            8425..>10022
                     /locus_tag="VFM00_07455"
     CDS             8425..>10022
                     /locus_tag="VFM00_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7498121.1"
                     /translation="MRRVPRNGPRRRDRSQRQAAPRARAGQPSSLWPFAALAAALLVI
                     VLAVYAGAFHHDFVRWDDPTYVEENPLVLGRQAGPLLTAVVARNFHPVTMLSLALNAS
                     EPLSPRPFLVTNVVLHALNTGLVFWLALLLLRRRLLPAFFVALLFAVHPMHVESVAWV
                     SERKDVLYALFFLSAAAAYWKYLETRRRAWLAATFGLFLLSCFSKAVAVVFPAVMVLL
                     DYWKRRKLLERPALLEKVPFLAASVLFGLIAIDVQAGGDFHGMLNPVGERMRALPATS
                     PFPLLQRVAFPAYGHLQYLWKLFVPLRLSAFYRYPASVAESSGPEYVAGIVVLLATVA
                     LFLVSVRRWRIVAFGLGWYFATLLPVMQWLPVGEAIMADRYTYLPYFGLFLILVAGVA
                     AAAERLPALRVPLWSVCGLFALFLMARTLHQVETWKDTDALWSNVIRNDPRTDKAYAA
                     RANFRGRAGRVAEALSDLQIARNLNPRRGEVYEDLGNAYGSLGRPDSAVLFFGEALRI
                     DPTLGHTYYNRAIAYLRLGRPREA"
BASE COUNT         1628 a         3388 c         3349 g         1657 t
ORIGIN      
        1 gtcatcggga ccgagatgac ggcccgaggc acgctgccca gctggatcga gatgaacggc
       61 ccgcgccacg tgtcggcccg gtaccagccg gtctgatcgg cgagatacca cgagtcgttg
      121 tagcggtaca ggtcgtagct cgcgttgccg cggacgtagt cgatgtcggt gcccgggatc
      181 atgtcgtagt tcggctcggc agtgaaggtc cgtcccgagt tccagtagcg gaccgtctga
      241 tagcgatcgc ggtcgcggta gcggtcgctg tcccggtatc ggtcgctgtc gcgatcgtcg
      301 tcgtcggtag acgagacgct ccagtcgtgg cgatagcgcg acggaatggt gaggacctca
      361 cgcggaacgg cggatgcccg gatcgagagg aacggcccgc gccacgacgt ggagcgatac
      421 caaacaccgc cgtccaccag gtaccaggtg ccgtgcaggc gatagaggtc gaggtcggcg
      481 gcctggcgcg cgtacgtgac cgagctgttg ggaatgacgc ccatcgccgg cttggtcgag
      541 aacgtgcgtc ccgaagccca gtagccctcg ggcagattcc ggtaccgcgg atcgcggcgg
      601 tccacccgga ccgacaccca gttgcgccgg tactccgatg ggacggtcga gatctcatag
      661 ggaagaacgg caacgtccat cgaggcccag ggcccacgcc acgagccggc ccggtaccag
      721 cggccctgat cgttcaggta ccacgaacct tcgaactggt agacgtcgta gtcggacgcg
      781 tcgcggatgt aatacacgtc ggtgtcgccg acgcgccgca tccggggctg ggacgagaaa
      841 tggtagttgc tgtagccggc ttcatagtcg tcgcggtagg cgacatcgga acgcgtcgag
      901 gagcaccccg agagcgagat ggccggaagc gacaccagga gcgtcacccc cagagtccag
      961 attgcagatc ggttcatggg agtatttcct ccccgcgagg ccttccccgg caggggtgcg
     1021 gcgcgcggtt gtaggtatgc cgggtgcggc cggcgatatc tacaatcgcc gagcgcaagt
     1081 ggactctcat cctggcggtt ttcacgcccg cccaaggcgt aacgagatgc tttcaattcg
     1141 cgtgccaggg aggctagact gccgcgtccg ccgacgcaca acccaggcag gagggtccca
     1201 tggtgccgat cccgtccttg tggctgccgg tgctcctttc cgcagtgatc gtcttcctcg
     1261 cgagctggat cgcacacgcc gtgcttcccc atcacaagaa cgacgtcaaa cacgttcccg
     1321 cagaggacgc gttcctcgaa gcggtccgca agctggacct tccccccggg gactacatgg
     1381 cgccgcggcc ctccagcatg gcggccatga agaaccccga gttcgtcgag aagcggaagc
     1441 ggggtccggt ggtcatcatg acggtgatcc ccgggggccc tcccagcatg ggcaaggagc
     1501 tggggcagtg gttcctcttc acgatcgtgg tcggcatctt cgccgcctac atcaccggcc
     1561 atgccgtcga ggtgggcgct ccctacctgc gcgtgatgcg cttcgcgggc gcgaccgcct
     1621 tcttctgcta ctcgatcgcg cagttccccg aatcgatctg gtacaagcgg agctgggcga
     1681 ccacgttcaa gaacatgttc gacgggctga tctacgggct gctgacggga ggcgtcttcg
     1741 gctggctctg gccgcgctag cgacacactg cggcgcagag aaaaccccgg tcgcggggcg
     1801 ggcccgagag tccgccgccg cgccggggtt tcgtttttga cgcgatcgat cgaagggcca
     1861 cccgttacac agggaggctc cgatgccggt tgtgcgtgat cgcgcggtag atcgcgagca
     1921 gcaggacggc gccgatgatc gaggcgatga tcccggctcc ttcgccgcgg tcatagaggc
     1981 ccatggagcg gccgatgaat cccgcgagaa gggatccggc gataccgagg atgatcgtca
     2041 tcaagatgcc gccgccatcg cggcccggca tgatcagctt ggcgagtgcg ccaatgacca
     2101 atccgatgat gatggtccag agaatgttca tgattggctc ctgtccaccg gtccctcacg
     2161 gctggggacg gggactctgt cttgccctcg ttcaccgctt tgagcacccg ggcgatctcc
     2221 ctgatcgccg ggaccgcggc acccccgcgg ctgtgacctc atgccactgc aagctgcgga
     2281 ccacgcggtc cgatcaatcg cattcgacca ccccgcaagg gcttaggcat gcagctcccg
     2341 ggttgtcctt cctgcgccgg cggcgcgtgg cggtgtgctt tgtactcttg gagggtcggg
     2401 agggcgcccg cttcaagggg acgcgacggc gactcctcga acgcggacct gctgcacgtc
     2461 ggccagcttg aggaagagga actgcgggcg actgatccga tagtcggcca gggccaccgg
     2521 gaacgccgtc tggaattcga tccgatcttc cttgccgctt tgcttgtacg ccgcgaccac
     2581 ggtcaactgg atgcggtggg cgaccccgtg aagggtgaac gttccttcga cgtcgagggt
     2641 cacgggcttg ccggagctca acgcgccgct cccgggtcga atcgcgacgc cgtcgaagac
     2701 cgccttgggg taggtggcgg tttccagatg ggcctcgcgc atgtgctggt ttcgtttcgc
     2761 gattcccgtg tccaggctgg cgaggtccac ctcgaagtgt accgtggccg agtcgccgac
     2821 cgtcgcgggg tcgaccacga tgctgccctc cagccgactg gtctttccct cgaagcgctc
     2881 catggcggcc ctggacgtga acgagacctt gttctcgccg ccggggtgaa ccaggaacgt
     2941 cgccgcccgc cccgcgctgg gagcggtgat gaagagaagc gctgctgcga tcgctgatgc
     3001 cggtggtttc atgaggcgcc tcaatagagc agatggagca tgatgacggt ctcgatgagg
     3061 tcacggccgc tgtaggccac gccctcctcg taggcggtac gccggacgag cgcctcgcag
     3121 gccgcgtagg ggcgcggcat gaccgccacg ccgcctccgt atcgggactt ggcgccggat
     3181 gccttgtcgc ggtcgggatc gaagaaatcg taggtgcctt tgagctcgag gccctggcgg
     3241 acgagcacgg tcacctcgtg cgacgtgacc gcgccgatcg tggcggggcc gccggcgggc
     3301 cggttctgga agaagtcccc ctcggccagc cacgtgaggc gattccacgt gagatagccg
     3361 tacagccccc acgtgtcgta gtcgcgcgcg aaacccggct ggtgataggc ggcgccgccc
     3421 agcgacgcgc cgaccggacc gaggcgatgg cggaagacgg cgttcaccac gcccgccacc
     3481 ttggtatcgc tgtccatgac gaggccgcgg tttccgttca cgaccgccgc ctggaggtcg
     3541 agcgggcccc gcgcgtaccc agcctccagg cccacgtcgc tgttggcggg cggcatgaac
     3601 cccagctccg cgcgcacgaa catcgtgtgg tcgtcgaact tccatccata ggaggggacg
     3661 aagcggcccg ccttcacgta cagctcgggg cgcaggtaat ccaggccgaa cagctcgtag
     3721 ctgccggact cgccgcggtc gtagtagagc gccacggtgg agtcgagctg gaacgagaag
     3781 tacagatccg cctgcatctg gaagaagttc aggtggtcct ggggaaggtc cgcccccacg
     3841 tagacctcgc ggaaatcggt cccgacctgg acgtgccggg cgatcgaggg agccacatcc
     3901 tcgagcgccg gaagaccacc gcgccgccag gcgatctcct cgggaatgat cttctcggtc
     3961 gcataggagg tgcgcagccc tccgccggtc ggattgacgt ggcacagcgc acacgtctgc
     4021 tgatagcggg ccgagtagcg cggaagcgcc acgcccggcc gagccgcgag aagacccagg
     4081 ccgagcagcg cgagaccgac cacaagaagc cggatcatgt ccctcctctc aattgttgcg
     4141 cgccccttcc aggatccagt cccggatcgt gtcgcggtcc acctgaggca ggagaggccc
     4201 tccctggggc atcagctgcc cgtactggcc cgagttggtg atctttccgt acagaaccga
     4261 attcgtgagg tcgaacggga tgacgcgcac gccgggcgga tacgtgaaaa tgcgatcctg
     4321 ggtcaggatc cggtagcccg agcccggatc gctgaacgcg tggcaaccgt cgcaattctg
     4381 gaggatcggc tgcacgtcgt tcgcgaacga gacggtcgtc gcgaagccgg agatcggaac
     4441 gtcggggatt cccggaccca gagaaaccga gcacgactgc ccccgcccgg tcgtcggcgc
     4501 gaactgaacc gagaccgtga ccgagccccc cggcgggatc gccgccgcgc cgtcccccga
     4561 caggatctgg aaggcgccgc acgagcccga cacgttcacg ttgagcggcg cggtgcccac
     4621 gctgaagatc gtgaacgttc gggtcgcggt ctcccccgcc gcgaggacgc cgaagtcgag
     4681 cgaatcggga agcacgatcc accgggcccc gggtgtctga agcgcgcccg atccggacag
     4741 cgccacgcgg ccggacggaa agccgagatc gaggtcgcag gagaacgcgc cctcggccgc
     4801 cggatgaaag cgcagtcgaa tcgtgagcga tccgccggga gggatgctga agggaccgcc
     4861 ccccgacacg atggcgtatt ccggacacga aacctgggcg tttccgagga gcgcgcccgt
     4921 tccggcgttg gtcacggtca cttcccggaa cgcggaatcg gccgcggtga cggcgccgaa
     4981 gtcgagggtt tccgcggaaa ggagaggctg gggtaggggt cgcagcgggc gccccaggtc
     5041 ggagcacccg gaacaggcca gtgcggtggc tatgacgacg atagctaaat ggcgcgaggg
     5101 tcgcatgatc ccgggaattc ctcgtttctc gtcagctttc ggtggggacg cacgagtatg
     5161 caggagccta ggcagcgcct gctaggggat gcagtgacct ggatcaccga gaaaacaggg
     5221 ttcccatcgg cctcgcctgc atctaagata ggttcgctca cacgagccaa tcccgtcgtc
     5281 catactcgaa acgcgcaggt ctcccatgcg gctcctcgtc ctggccacac gactgctcct
     5341 gctttcctcc ttcctcgcgg ccggctgcgc cgtatcccgg cccgtcgccc gcctggactc
     5401 cacgccggtc tccgccgagc tcgcgatcct gcccaccccc gtgccggatc tggggggagc
     5461 cgacatctcg acgggcccgg aggacgcccc tgcccccgcc ccaaggcctc atcgggcgct
     5521 cacgcgggga caggtcggaa ccgcttcctt ctacgacgcc tcgttcaacg ggcagaccac
     5581 ggccaacggg gacacctacg acgacgccaa gctcaccgcc gcccaccgca cgctggggtt
     5641 cgggacgcgg gttcgcgtca cgaacctcga gaaccaccgc tcggtggtgg tgacggtgaa
     5701 cgaccgcggt cccttcgtcg gaggtcgtgt catcgacctc tcgcgacggg ccgcgcgcgc
     5761 gctggacttc gtcgaggatg gcacgacccg ggtccgggtc cagcctctgt agctgctata
     5821 atcgtggggt gatcgcctcg cgccgaagca ccctctccaa ggctttcgcg ctcgcgccgg
     5881 ccgtcgtcat ggccctggcc gcgagcgctc catcctgctt cgccctcacc ctgcacgtcg
     5941 gtcccggaaa gtcctacgcc acagtccgcg ccgcggccga agcggcgctc cccggagacg
     6001 tggtcctgat cgacgcgggg gtctacagcg gcgacgtggc cacctggaaa tccgacaacg
     6061 tcaccgtccg ggccgtcggc gggcgggctc atatgaaggc cgacggagcg caggaggggg
     6121 gcaaggcgat ctgggtggtg caggggcgga acttcaccgc cgaagggatc gagttctccg
     6181 gagccaaggt tcccgaccag aacggcgccg ggatccgctc cgaaacgccg ggaacgctga
     6241 tcctgcgcga ctgctatttc cacgacaacg aagagggcat cctgggcggc gccgattcca
     6301 tggtcatcga gaactgcgtc ttcgaccgga acggccaggg cgacggacgc acccacaaca
     6361 tctacgcctc gggacgctcc ttcacgcttc gaggctcgac cgtgaagcgc gcggtgatcg
     6421 ggcacaacgt gaagacccgc gccaagaaca actacatcct ctacaaccgc attctcgacg
     6481 aggccagcgg cacggccagc tacgaggtgg acgtgcccga ctgcgggcgc acctatctca
     6541 tcggcaacgt catcgagcag gggcccaagt cggagaacgc cacgatcatc tcatacgggg
     6601 ccgagagcgc gtcgaacgtg caggacctct acgtcgtgaa caacacgatc gtgaacgatc
     6661 gccaggacgg gacgtttctc cagatccgcg acggcacgaa ggctctcgtg ttgaacaaca
     6721 tcttctatgg gcccgggacg ccctgggcgg gcgggcgcgt ggtggagagc cggaactacg
     6781 tggagccttc catgcataac ggccccggct tcgccgatcc gcgcaccttc gattaccggc
     6841 tcaccgcttc ctcgcctccg gagatcgtgg acgcgggcgg tgcggtcggt ctctccatca
     6901 ccggcttcga cctgaccccc accggccagt gcggcgacga cgcgcgcgtg cgcgcgcgtg
     6961 cccgggccgg cgcgctggac atcggcgcgt tcgagcggga acccgcgaag ccctagctac
     7021 cccagattcc cagcgcgcgc cgcaccagga cgagctgccc gatgtggtag gcgttgtggt
     7081 ccgccacgac gaggagctcg cgcaggtagg tctggccggt tccgttggga acgcggccga
     7141 agaggtcgac ggaggggtcg cgggccagcg ccgcgagcgc ctcgaggtcg gcatggaacg
     7201 cggcgacgct ccggtcccac gcggcgtccg actcgggagc gggcgatttc ggccagtagt
     7261 cctccggcca cttcatctcc tcgtacttcg gcgccgtgca gaactcgagg atgtcgcgct
     7321 gcgcgagccg gagatgctcc aggagctgcc acggcgaatg gggcattccc tgcggtacgg
     7381 cgccccgatg ccggggtgcg attcctccga cggcggcatc gaacccggcg tgagcgtctt
     7441 cccaggcgag aagccgcgcg atctgctcgc gaagcggcgt gtccttgtcc atggcgcgga
     7501 tcatagcacc cggctggaac gggcggcgag gacggggtac catcccgcgt cgcgccccga
     7561 cccgggggcc ggcccgcggc ggacaagccg cagcatcgga ggcgacgtga cgacctcacc
     7621 ggagcaaccg ggcgccggcg gagacgaccc gctgctcgcc taccgcgacc ccgcctttct
     7681 cgacagcgag gcggcccggc cgatccggat catctcggaa tacctggccc cgctggacgc
     7741 gttctgccgg gagtgcgtga gcgacaccat cgtcttcttc ggctcggcgc ggatcgcccc
     7801 ggacggcccc atgggccgct attacgccga ggcgcgggag ctcgcgcgtc tcatcaccga
     7861 gtggtcgcgg agcctgaaat ccccggggca ccgctacatc gtctgctccg gcgggggtcc
     7921 cggaatcatg gaggcggcca accgcggcgc ccaggaggcc ggcgggcgaa ccatcggcct
     7981 caacatcggg ctcccccacg agcagcgccc gaacccttac atcaccccgg ggctcagctt
     8041 cgaattccac tacttcttca tgcgcaagct ctggttcgcc cacctggccc gggccatggt
     8101 cgtctttccc gggggcttcg gcaccctgga cgaaacgttc gagatgctca cgctgtctca
     8161 aacacggaag ctggaccgct cgctgccggt gctgttgtac ggctcgagct actggaagga
     8221 gctgatccag ttcgaggcgc tcgtccggca cgggatgatc gaccggagcg atctcgacct
     8281 gttcgtgttc gtggacgagc cgcgggaagc actcgacatc ctgcgccagc ggctgggcga
     8341 ggagaagaag ccccccgcgc cggccttcgc cgactcgacg actccggaga gccccatgcc
     8401 gacaccgcct cgaggcccgc gcgggtgagg cgagtgccga ggaacggtcc ccgccgccgg
     8461 gaccggtcgc agcgccaagc ggcaccgcgt gctcgagcgg gacagccctc gagcctgtgg
     8521 ccgttcgcgg ccctcgcggc cgcccttctc gtcatcgtcc tggccgtcta cgcgggtgcc
     8581 ttccatcacg atttcgtgcg atgggacgac ccgacctacg tggaggagaa cccgctcgtc
     8641 ctcggccggc aggcgggacc gctcctgacc gcggtcgtgg cccggaactt tcatccggtg
     8701 acgatgcttt cgctggcgct caacgcgtcc gagcctctct cgccgcgtcc gtttctggtg
     8761 acgaacgtcg tcctgcacgc tttgaacacg ggactggtct tctggctcgc gctgctcctc
     8821 cttcgccgga ggctgctccc cgcgttcttc gtcgcgcttc tctttgccgt ccatccgatg
     8881 cacgtcgaat cggtggcctg ggtatccgag cgcaaggacg tgctctacgc gctcttcttt
     8941 ctctccgcgg cggccgccta ctggaagtac ctcgaaacgc ggcgcagggc ctggctggcc
     9001 gccaccttcg gcctcttcct gctctcctgt ttctccaagg ccgttgccgt cgtattcccc
     9061 gcggtgatgg tcctcctcga ttactggaag cggcggaaac tcctcgagcg gcccgcgctg
     9121 ctcgagaaag tcccctttct cgcggcctcc gtcctcttcg gcctgatcgc gatcgacgtg
     9181 caggcgggcg gcgatttcca tgggatgttg aacccggtgg gcgagcggat gcgggccctg
     9241 cccgcgacca gtcccttccc gctcctccag cgcgtggcgt ttcccgccta tgggcatctc
     9301 cagtacctct ggaagctgtt cgtgcctctc cggctcagcg cgttctatcg ctaccccgca
     9361 tcggttgcgg agtcctccgg accggagtac gtcgccggaa tcgtggtgct tctggcgacc
     9421 gtcgcgctgt tcctcgtgag cgtccgtcga tggcgaatcg tcgccttcgg tttgggctgg
     9481 tacttcgcga ccctcctgcc cgtgatgcaa tggctcccgg tcggcgaggc gatcatggcg
     9541 gatcgataca cctatcttcc ctatttcggt ctcttcctta tactggtcgc cggcgtcgcc
     9601 gcagcggccg agcggctccc ggcactccgc gttcccctgt ggtcggtgtg cggtctcttc
     9661 gcgttgttcc tgatggcgcg gaccctgcac caggtagaaa cctggaagga tacggatgcc
     9721 ctttggagca acgtgatccg gaacgacccg cgcaccgaca aggcctacgc cgctcgagcg
     9781 aacttccgcg gacgcgcggg gcgggtggcc gaggcgctga gcgatcttca gatcgcgcgg
     9841 aatctgaatc cacggcgggg cgaggtctac gaggatcttg gaaacgcgta tggctcgctg
     9901 ggacggcccg actccgccgt gctgttcttc ggagaagcgc tgcgcatcga cccgacgctg
     9961 ggtcatacct actacaaccg cgccattgcg tacctgcgtc tgggcaggcc ccgggaagcg
    10021 tt
//
LOCUS       DASSLZ010000190        11469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1836728, whole genome shotgun sequence.
ACCESSION   DASSLZ010000190 DASSLZ010000000
VERSION     DASSLZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836728"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VFM00_07460"
     CDS             <1..844
                     /locus_tag="VFM00_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7498122.1"
                     /translation="KAAELAFFRGDLTKVRTLLTSLLTAIPNHEAALRRLVDITSQAE
                     DRPAEAKARFDLAEVYLAHEEWPLARAEYMRVLELNPGNDRARARVQRLDAMNGDAPA
                     GIDLDAIDSPKPTASVRVRDEQPASMDNLVDLEEIIDEFKAGVSNTISGEDHESHYDL
                     GMAYMEMGLHDEAIQEFQIASKGGPMELKCLEMIALCYLEKNEPAAAARELDRALQLP
                     GHGPEETISIRYNLGLANERLGNLDKALQHFEEVYLLNVDFLKVAAKVREVKARLAGA
                     QDPT"
     gene            841..1689
                     /locus_tag="VFM00_07465"
     CDS             841..1689
                     /locus_tag="VFM00_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET7498123.1"
                     /translation="MTGSTIDLHMHSTASDGSDAPEAVAALAERNGLRVIALTDHDCL
                     DGIPAAAARAEAAGIRLIPGAELSVHEEGTDVHLLAYGFDPLDVALVEAIGRYRDARR
                     ERARKILARLKGLGIRVSLETVEEIAKGGALGRPHVAEALLRAGHVETFNEAFQRFLG
                     THAPAYVAKAVVRLEEATRVVREAGGVTVLAHPGTLNRDHLIPGWARRGLDGIEVWHS
                     KHDANAVARYQGYAKIHGLLMTGGSDYHGERTPSVTIGSVPVPESVLPPLDEAFRARR
                     PAASGL"
     gene            1766..2311
                     /locus_tag="VFM00_07470"
     CDS             1766..2311
                     /locus_tag="VFM00_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498124.1"
                     /translation="MRTAVRSAALIAFSVACGYAKLILFPYLFFVELFSVAVFLSGVI
                     VGVGWGMWVAAAARLIFSVANPYGPPHPWVLAAQVVGAALLGALGGLARRALAASWLQ
                     AAPARAAFLGTCGLLGTTVYDLLTNLAQGIAFGAPRATLALAALPSLQHIASNTAIFV
                     IIGGLAASWLRRDPRLLSHAA"
     gene            2301..4466
                     /locus_tag="VFM00_07475"
     CDS             2301..4466
                     /locus_tag="VFM00_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498125.1"
                     /translation="MRPRPLLAALLVAVLAAPSFARAQPAAPAESTVTRPMPLVDSTS
                     VVPGGPVPVLDSTVVIPAMARNAPGKAPPESLPRPDTLGLTLRADRLRKRGDVSLTQL
                     LQGRRPVWIETAPGMAPLFGAPRLLDSGDPVSPDPAPIAADRATDRTAIADFTHLDRS
                     GYVLGVPGFATAWEIPETRGIDAFDEVAIDSSLAPQAFLGAGDLFARPGSVPFAALAM
                     PDPPAPYRVRSALLYRKGAGNLLDTGARFSSPLFAHGIAGSYVRHASEALTPFLSSIS
                     TRYTLAAGLTRGGPLRSWVEGRLFKMRMETDYPGGYGNPDLGFTLPTHARAEWASREA
                     ALHALWTGGGMVASAALRAGKSQATQIRYDLGRERWAFPEIAAEARLSGGGPRATEGG
                     VDSLANVPAPSWSWSLEGRGSRRRVDYRSDDSAFLPRIRAGRAAATLRRAGPEGGAEA
                     TAALDFREGDPSLVDGRLSLWGARGHARFRLDAESAHERPTLVDLYTPARIDTMPSFV
                     APTLLLVRGGDRALGARSLRGVLGVAAFRANPGVELVAFGSARRVTGDFGWTAERSVF
                     GDTILIVDAARERGDGWAFQAAAGGDAAFGPFTARGLGWVRGGTDGISPQAGAPPRAG
                     LDASLGLRATFFQGDLPLELELAAHATGPRHGILTAPALATWDARVHADFGSAGLFVT
                     ITNLFDASVPSAVYLIDENRGAPLPGRALTAGVVWHIQD"
     gene            4491..4937
                     /locus_tag="VFM00_07480"
     CDS             4491..4937
                     /locus_tag="VFM00_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7498126.1"
                     /translation="MRAGLIFVLVSVTAGGAFRGWQREHRERFDEIVASLVEADARDA
                     SSRTPLRATASDSVGSTAVSRIPAAGSNRPARRAAAAPLRPASIDVDRASEAELLRLP
                     GIGPALAGRIVAERASGGAFGGPDGLLRVHGIGPKTLEKIRPYLTP"
     gene            5265..6962
                     /gene="pilB"
                     /locus_tag="VFM00_07485"
     CDS             5265..6962
                     /gene="pilB"
                     /locus_tag="VFM00_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HET7498127.1"
                     /translation="MQQENVGLKLLEARLISPEHLQKAQQQVKTGGGNLTAALVKLGA
                     IAEEDLIKFLAELYGVPSVDLSNFQPDPAVVKLLPSDVASKFQVVPIARAGRRLTVAM
                     SNPSNIFAIDDIKFITGCEVQPVVAPEAAVKKAIDKAYDSAASFETVMKGLDDEIEVI
                     DEGPDDVPDVALLGEAEQAPVIKLVNSLVSDAVRKGASDIHIEPYEKSLRVRFRIDGT
                     LYEMMSPPFRMKAAITSRLKIMAELDIAERRVPQDGRIKLRLLGRTIDLRVSSLPTIF
                     GEKVVMRILDKGNLNIDLSKLGFQEQAYTDFSRAIAQPYGMVLVTGPTGSGKTTTLYS
                     ALSKINVPEVNIMTAEDPVEYNLEGINQVLVNDDVGLSFAAALRAFLRQDPNIIMVGE
                     IRDIDTGSIAVKAALTGHLVLSTLHTNDAPSTINRMVDMGIEPFLVSSSTNLIMAQRL
                     LRKVCNSCKTDVKLHPEALRELGLSDEDARKESFKEGKGCVDCSNTGYKGRVGVYEVM
                     PMSARIRDQILDRASTADIKKQAVAEGMLTLRMDALIKLKKGITTAEEVLKETASDR"
     gene            6980..8059
                     /locus_tag="VFM00_07490"
     CDS             6980..8059
                     /locus_tag="VFM00_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET7498128.1"
                     /translation="MVSLRQLLDEMTKMRASDLHISAGVPPQVRVDGSIVPMSHPPLT
                     PQMTQQLAYSVLTEEQRKRFETTKELDLSFGVPGLSRFRANVYLQRGVTSMAIRQIPY
                     EILTFEQLGLSKTVRDFTTKQKGLVLVTGPTGSGKSTTLAAMIDLINSNRAGHIITIE
                     DPIEYVHHHKKCIVHQREINSDTTTFVTALKYVLRQDPDVILIGEMRDLETIAAALTI
                     AETGHLVLATLHTNSAYESINRIVDAFPSNQQQQILSQLAFVLEGVMTQQLIPRAKGP
                     GRVMVSELLICTPAVRAVIRERKIHQIYSLMQAGTKHGMQTMNQALFQAYVNKLISLD
                     EALGRSTDVKELEGMLEKVGVHVAA"
     gene            8089..9312
                     /locus_tag="VFM00_07495"
     CDS             8089..9312
                     /locus_tag="VFM00_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7498129.1"
                     /translation="MPVYVWKGRTTGGEIQTGELTLDSQEEALAALRKKRIIITSVRE
                     KKGDVKLTLPKFGTGVSTRDLAIFTRQFATMINAGLPLVQCLDILSKQTEKENFRAII
                     AQVMREVEAGNTLAESLAKKENNRVFDELFVNMVEAGEAGGILDDILQRLATFIEKAE
                     ALKRKIQGAMVYPAVVMTVAILATAFMLIFIIPTFAKMFTGFGAELPLPTKIVMGLSS
                     FLRSYWWVMLLGVVGTFVGIQRYYRTERGHIAIDRFLLRIPVLGDVIRKGAVARFTRT
                     LGTLISSGVPILTGLEITARTSGNRVIQEAIMAARASIREGETIAAPLRVSNVFPPMV
                     VQMISVGEETGALDDMLTRIADFYDDEVDTAVEALTSLIEPIMIVFMGSVVGGMVIAM
                     YLPMFKLINVVASGH"
     gene            9320..11038
                     /locus_tag="VFM00_07500"
     CDS             9320..11038
                     /locus_tag="VFM00_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7498130.1"
                     /translation="MELARPPLAPGPDEALRREVRAVIVGRIALITLVTAMAAAFSDR
                     IAEPAQNVPLGFLVALSAGLAVAYHRAAKSGAPLRSLLAMQLIVDVAVISYLLLFTGG
                     ASSPFVPLYLLAPLLGGVFLSVQGGMLLAVAGAVAYAVLYAAERGGMLPPVSYGLTER
                     LSESALKLRLVLYLPLLFVVGAIGGALGRTLREGKKALDEARAELSRALFDTETILEN
                     LSSGLASVDAHGVVRHWNRAAGEILGRPASEVRGRHYETALGEGFEEFHARIRETLAS
                     GVPAARHEARVTTRGGRTVPLGISTSVLVDAEGGRRGVIALFQDLSDVKALEDRIRRG
                     ETLAAIGELSAGIAHEIRNCLNPIAGSVEVLQRELQVTGENARLLELIVRESERLDNF
                     IRELLDYARERPLKTERVDLAALTRETAEVARRHPSMGAGKTVTVEAPEKPVWAQVDF
                     EQTKQVLLNLVINALEAIEGAGSVTARVDPANGSRSRGRAGFAAIEVRDTGAGIRADD
                     LRQVFEPFFSTKLGGTGLGLAIANRIVERHGGAVEIESRVGVGTTLRLWLPRAERPAK
                     TVARAA"
     gene            11065..>11469
                     /locus_tag="VFM00_07505"
     CDS             11065..>11469
                     /locus_tag="VFM00_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7498131.1"
                     /translation="MAGEKILIVDDEESMVQFLSILLTKEGYDVHVARSGKDALEQAE
                     GENFDVVVTDIKMPGEIDGLGVLSGVKKIDPSVPVVIRTAFASQKSAIEALNRGAYQY
                     LEKSAKNDEIRLVIKNALAMRRVQSENVLLKRQ"
BASE COUNT         1764 a         3932 c         4026 g         1747 t
ORIGIN      
        1 gaaagcggcg gagctcgcgt tcttccgcgg cgacctcacg aaggtgcgca cgctgctcac
       61 gtcgctcctc accgccattc cgaaccacga ggccgcgctg cggcgcctgg tggacatcac
      121 gtcgcaggcg gaggaccggc ccgccgaggc caaggcgcgg ttcgacctgg ccgaggtcta
      181 cctggcgcac gaggagtggc cgctcgcgcg cgccgagtac atgcgcgtgc tggagctgaa
      241 tcccggcaac gaccgcgccc gcgcccgcgt gcagcgtctg gacgccatga acggcgacgc
      301 cccggcgggg atcgacctgg acgcgatcga ctcgccgaag ccgaccgcgt cggtgcgcgt
      361 gcgggacgag cagcccgcca gcatggacaa cctcgtggac ctcgaggaga tcatcgacga
      421 gttcaaggcg ggcgtatcga acaccatctc cggggaagac catgagagcc attacgatct
      481 cggcatggcc tacatggaga tgggcctcca cgacgaggcg atccaggagt tccagatcgc
      541 ctcgaagggg ggaccgatgg agctcaagtg cctggagatg atcgccctct gctatctcga
      601 gaagaacgag cccgccgccg cggcgcggga actggaccgg gcgctgcagc ttcccggaca
      661 tggaccggaa gaaaccatca gcatccggta caatctgggc ctggccaacg agcgcctcgg
      721 caacctcgac aaggcgctcc agcatttcga ggaggtgtac ctgctcaacg tggatttcct
      781 gaaggtggcg gccaaggtgc gcgaggtgaa ggcgcgtctc gcgggcgccc aggatccgac
      841 atgaccggat cgacgatcga cctgcacatg cactcgaccg cctccgacgg ctcggacgcc
      901 ccggaggcgg ttgccgcttt ggccgagcgc aacggcctgc gcgtgatcgc gctgaccgac
      961 cacgactgcc tggacggaat cccggccgcg gcggcgcgcg cggaggccgc cggcatccgg
     1021 ctgatccccg gcgcggagct ctcggttcac gaagagggaa ccgacgtgca cctcctggcc
     1081 tacggcttcg atccgctcga cgtggcgctg gtcgaggcga tcgggcgcta ccgcgacgcg
     1141 cggcgggagc gcgcgcggaa gatcctggcg cggctgaagg ggctggggat ccgcgtgtcg
     1201 ctggagacgg tggaggagat cgccaagggc ggcgcgcttg gacgccccca cgtggcggag
     1261 gcgcttctgc gcgccgggca cgtcgagacc ttcaacgaag cgttccagcg cttcctgggg
     1321 acccacgccc cggcgtacgt ggccaaggcg gtcgtccggc tggaagaggc gacgcgcgtg
     1381 gtgcgcgaag cggggggtgt gacggtgctg gcccacccgg gcacgctcaa tcgcgaccac
     1441 ctgatccccg gctgggcgcg ccgcgggctc gacgggatcg aagtctggca ctccaagcac
     1501 gacgcgaacg cggtcgcgcg ctaccagggt tacgccaaga tccacgggct gctcatgacc
     1561 ggaggatcgg actatcacgg cgagcgcacg ccctccgtga cgatcggctc ggtgccggtc
     1621 cccgaaagcg tcctgccgcc cctggacgaa gcgtttcgcg cccgccgtcc cgccgcctcc
     1681 ggcctctagc cgccttcgcc gccgccgcgg caccgcgtcc ttgcgcccga cggcgcgcct
     1741 ctggtagcgt ccctgtcccc ggcccatgcg caccgccgtc cgtagcgccg ccctcatcgc
     1801 cttttccgtc gcgtgcggct acgcgaagct gatcctgttc ccgtatctct tcttcgtgga
     1861 gctcttctcg gtggcggttt ttctgtcggg ggtgatcgtg ggcgtggggt gggggatgtg
     1921 ggtggcggcc gcggcgcggc tcatcttcag cgtcgccaac ccctacggtc cgccgcatcc
     1981 ctgggtcctc gcggcccagg tcgtgggcgc cgccctgctc ggggccctgg gcgggctggc
     2041 ccggcgggcg ctcgccgcgt cgtggctcca ggccgctccg gcccgcgcgg ccttcctggg
     2101 gacgtgcggc ctcctcggga cgacggtcta cgatctgctc accaatctcg cccaggggat
     2161 tgccttcggc gcgccccgcg cgacgctggc gctggccgcc cttccttcgc tgcagcacat
     2221 cgcatcgaac accgcgatct tcgtgatcat cggcggcctc gccgcttcct ggctgcgccg
     2281 cgatccgcgg ctcctgagcc atgcggccta gaccgctcct cgcggcgctg ctcgtggcgg
     2341 tgctggccgc gccgtcgttc gcccgggcgc agcccgcggc gccggcggag agcacggtca
     2401 cgcgcccgat gccgctcgtc gactcgacct ccgtggtgcc ggggggcccg gtcccggtgc
     2461 tcgattccac cgtcgtcatc cccgcgatgg cgcggaacgc tcccggcaaa gcaccgcccg
     2521 agtccttgcc gcggcccgat accctgggcc tgacgctccg cgccgaccgg ttgcggaagc
     2581 gcggcgacgt gtcgctgaca cagctcctcc aggggcgccg tccggtctgg atcgaaaccg
     2641 ccccggggat ggcaccgctc ttcggcgcgc cgcgtctcct cgactccggc gatcccgtgt
     2701 cgcccgatcc ggcaccgatc gccgcggatc gcgcgaccga tcgcaccgcg atcgccgatt
     2761 tcacccatct ggaccgatcc gggtatgtgc tgggcgtgcc cggcttcgcg acggcgtggg
     2821 agatacccga gacgcgcggg atcgacgcgt tcgatgaggt cgcgatcgat tcctcgctgg
     2881 cgccccaggc gtttctcggg gcgggcgatc tcttcgcgcg accgggatcg gtcccgttcg
     2941 cggcgctcgc gatgcccgat ccgccggcgc cctaccgcgt gcggagcgcg ctcctctatc
     3001 gaaagggcgc cgggaacctg ctcgacacag gggcgcgctt ctcgtcgccg ctcttcgcgc
     3061 acggcatcgc gggctcgtac gtccggcacg cttctgaggc gctgacgccg ttcctgagct
     3121 ccatctccac gcgctacacc ctggcggccg ggctgacgcg cggcggcccg ctgcgctcct
     3181 gggtggaagg acgcctcttc aagatgcgca tggagacgga ctacccgggc gggtacggga
     3241 accccgacct cggcttcacg ctgccgaccc acgcgcgcgc cgagtgggcg tcgcgcgagg
     3301 cggcgctgca cgcgctctgg accggcggtg gcatggtcgc atcggcggcg ctgcgcgcgg
     3361 ggaagtcgca ggcgacgcag atccgctatg acctgggacg cgagcgctgg gctttccccg
     3421 agatcgcggc cgaggcgcgg ctctccggcg gcgggccgcg cgccaccgag ggcggcgttg
     3481 attcgctggc gaacgtcccc gcgccctcgt ggtcctggtc gctcgaaggg cgcgggtcgc
     3541 ggcgccgcgt ggactaccgc tccgacgaca gcgccttcct cccgcgcatc cgcgctggac
     3601 gcgcggcggc gacgctgcgg cgggcgggcc cggaaggagg cgccgaggcc acggcggcgt
     3661 tggacttccg ggaaggcgac ccctcgctcg tcgatggacg gctctccctc tggggggcgc
     3721 gcgggcacgc gcgcttccgg ctggacgccg agtccgcgca cgagcgcccc acgctcgtgg
     3781 atctctacac ccccgcccgg atcgacacga tgccgagctt cgtggccccc accctcctgc
     3841 tcgtgcgcgg cggagacagg gctttgggcg cgcgatcgct ccgcggcgtg ctcggcgtcg
     3901 cggcgttccg cgcgaatccc ggcgtcgagc tcgtcgcctt cggctcggcg cgccgcgtga
     3961 ccggcgattt cggctggacg gccgagcgct cggtgttcgg cgacaccatc ctcatcgtgg
     4021 acgcggcgcg ggagcgtggg gacggctggg cgttccaggc cgcggcggga ggcgacgcgg
     4081 ccttcggccc cttcacggcg cgcggcctgg gctgggtccg ggggggcacc gacggaatct
     4141 cgccgcaggc cggggcgccg ccgcgggcgg gactcgacgc gtccctgggg cttcgcgcga
     4201 ccttcttcca gggggacctg ccgctggagc tggagctggc cgcgcacgcg acaggtcccc
     4261 ggcatggaat tctcaccgct cccgcgctcg cgacgtggga tgcgcgcgtc cacgccgatt
     4321 tcggctcggc cggccttttc gtcaccatca cgaacctgtt cgacgcgtcg gtcccgagcg
     4381 cggtctacct catcgacgag aatcgtgggg cgccgcttcc cgggcgcgcg ctcaccgcgg
     4441 gagtggtctg gcatatccag gattaggagg cggggatgtg gggccgggag atgcgcgcgg
     4501 ggctaatctt cgtgctggtg tcggtgacgg cgggcggcgc ctttcgtgga tggcagcggg
     4561 agcatcggga gcgattcgac gagatcgtgg cgtcgctggt ggaggcggat gcgcgggatg
     4621 cctcttctag gacgcctctc cgagcgacgg cgagcgattc ggtggggagc acagcggttt
     4681 cccgcatacc ggccgctggc tccaaccgtc cggcacgacg agcagcggcc gcgcctcttc
     4741 gtccggcatc catcgacgtg gaccgggcta gcgaggcgga actgctccgg ttgcctggga
     4801 ttggcccggc tctggcgggg cggatcgtgg cggagcgggc ttcgggcggg gcgtttgggg
     4861 gacctgatgg tttgctacgg gttcatggga ttgggccgaa gacgctcgag aagattcgac
     4921 cctacttgac gccttagcct ggttccgcgc cggtgggtgg ctcggccagc ctgcggctga
     4981 cggtgccggc gcggtacatg ggcattttga acgatgctct tcgcacggcg cacggcggat
     5041 tgcgccttgg cgggcgcgcc gcagcgtccg cacggtccat ctaacctcgt ccgccccgcc
     5101 cccttcgccc gcctcccgcg cctctgccgt ccgtggcacc cctcttgcga atccgaaatc
     5161 ggcgcgtttc cgcgcgttcc gcgaaagatc gcgggccggg gcgccgaaga ctccctccgt
     5221 acacgaacgc tcgtctcgtc tcgacacaac cgagggctac gtctgtgcag caggagaacg
     5281 tcggtctcaa gctgctggaa gcgcgtctca tttcgcccga gcacctccag aaggcgcagc
     5341 agcaggtgaa gacggggggc ggcaatctca cggccgccct cgtcaagctg ggcgccatcg
     5401 ccgaggaaga cctgatcaag ttcctcgccg agctctatgg cgtcccctcg gtggatctct
     5461 ccaatttcca gcccgatccg gccgtcgtga agctgctccc ctcggacgtc gcgagcaagt
     5521 tccaggtggt cccgatcgcg cgcgccggac gccgcctcac ggtggccatg tccaatccgt
     5581 cgaacatctt cgccatcgac gacatcaagt tcatcaccgg ctgcgaggtg cagccggtgg
     5641 tcgcgcccga ggcggccgtc aagaaggcga tcgacaaggc gtacgactcc gccgcttcct
     5701 tcgaaacggt catgaagggg ctggacgacg agatcgaggt catcgacgaa gggccggacg
     5761 acgttccgga cgtcgcgctc ctgggggaag ccgagcaggc gccggtcatc aagctggtca
     5821 actcgctggt ctccgacgcc gtgcgcaagg gggcgagcga catccacatc gagccctacg
     5881 agaagagcct gcgcgtgcgc ttccgcatcg acggcacgct ctacgagatg atgtcgccgc
     5941 ccttccgcat gaaggccgcg atcacctccc gcttgaagat catggccgag ctggacatcg
     6001 cggagcggcg cgtgccgcag gacgggcgca tcaagctgcg cctcctgggc cgcacgatcg
     6061 atctccgcgt ctccagccta ccgaccatct tcggcgagaa ggtcgtcatg cgaatcctgg
     6121 acaagggcaa cctgaacatc gacctgagca agctcgggtt ccaggagcag gcctacaccg
     6181 acttctcccg cgcgatcgcg cagccctacg gcatggtgct cgtgaccggt ccgaccggtt
     6241 ccggcaagac gaccaccctg tactcggcgc tctccaagat caacgtgccc gaggtcaaca
     6301 tcatgacggc cgaggacccg gtcgagtaca acctcgaggg aatcaaccag gtcctcgtga
     6361 acgacgacgt ggggctctcc ttcgccgcgg cgctccgcgc ctttctgcgc caggacccca
     6421 acatcatcat ggtgggcgag atccgcgaca tcgacacggg ttccatcgcc gtgaaggcgg
     6481 cgctcaccgg ccacctcgtc ctctccacgc tccacaccaa cgacgcgccc agcacgatca
     6541 accgaatggt cgacatgggg atcgagccgt tcctggtctc ttcctcgacc aacctgatca
     6601 tggcgcagcg gctcttgagg aaggtgtgca actcctgcaa gaccgacgtc aagctccacc
     6661 cggaggcgct ccgcgagctg ggcctgtccg acgaggacgc gcgcaaggag agcttcaagg
     6721 aggggaaggg ctgcgtcgac tgcagcaaca ccggctacaa ggggcgcgtc ggcgtctacg
     6781 aggtgatgcc catgagcgcc cggatccgcg atcagattct cgaccgggcc tccaccgccg
     6841 acatcaagaa gcaggcggtg gccgaaggaa tgctcacgct gcgcatggac gcgctgatca
     6901 agctcaagaa aggaattacg acggccgaag aggtcttgaa agagacggcg tccgaccgct
     6961 aggccacccg ggaggggaga tggtttcgct tcggcaactg ctggatgaga tgacgaagat
     7021 gcgggcgtcg gacctgcaca tttcggccgg cgtgccgccg caggtgcgcg tggacggatc
     7081 gatcgttccg atgtcgcacc cgccgctcac cccgcagatg acgcagcagc tggcctacag
     7141 cgtcctcacg gaagagcagc gcaagcgttt cgagaccacc aaggagctcg acctctcgtt
     7201 cggcgttccg ggactctcgc ggttccgcgc caacgtctac ctgcagcgcg gcgtgacctc
     7261 catggcgatc cgccagatcc cctacgagat cctcacgttc gagcagctgg gactgtccaa
     7321 gacggtccgc gacttcacga cgaagcagaa ggggctggtg ctggtcacgg ggccgaccgg
     7381 gagcggcaag tcgacgacgc tcgccgccat gatcgacctg atcaacagca accgggccgg
     7441 gcacatcatc acgatcgaag acccgatcga gtacgtccat caccacaaga agtgcatcgt
     7501 ccaccagcgc gagatcaatt ccgacacgac cacgttcgtc acggcgctga agtacgtgct
     7561 gcgccaggac ccggacgtga tcctgatcgg agaaatgcgc gacctcgaaa cgatcgccgc
     7621 ggcgctcacg atcgccgaga cgggccacct cgtgctcgcg acgctccaca cgaactcggc
     7681 gtacgaatcg atcaaccgca tcgtggacgc gttcccgtcg aaccagcagc agcagatcct
     7741 gtcgcagctc gcgttcgtgc tcgagggcgt catgacgcag cagctcatcc cccgggccaa
     7801 ggggccgggg cgcgtcatgg tctccgagct gctcatctgc acccccgccg tccgggccgt
     7861 gatccgggag cggaagatcc accagatcta cagcctgatg caggcgggga cgaagcacgg
     7921 gatgcagacg atgaaccagg cgctgttcca ggcctacgtg aacaagctga tctccttgga
     7981 cgaggcgctc ggccggagca ccgacgtcaa ggagctcgaa gggatgctcg agaaggtcgg
     8041 cgtccacgtg gccgcctagg ccgcccgggg acgaggggga acgtcgccat gcccgtctac
     8101 gtttggaaag gccggaccac cggcggggag atccagaccg gagagctcac gctcgacagt
     8161 caggaagagg ctctcgccgc gctccggaag aagcggatca tcatcacgtc ggtccgcgag
     8221 aagaagggcg acgtcaagct caccctcccg aagttcggga ccggcgtctc cacccgcgac
     8281 ctggccatct tcacgcgcca gttcgccacg atgatcaacg cgggcctccc gctcgtgcag
     8341 tgcctggaca tcctctcgaa gcagacggag aaggagaact tccgcgccat catcgcgcag
     8401 gtcatgcgcg aggtggaagc gggcaacacc ctggccgagt cgctcgcgaa gaaggagaac
     8461 aaccgggtct tcgacgagct cttcgtcaac atggtcgagg ccggcgaggc gggcggtatc
     8521 ctggacgaca tcctccagcg gctggccacc ttcatcgaga aggccgaggc gctgaagcgg
     8581 aagatccagg gcgccatggt ctatccggcc gtggtcatga ccgtggccat cctggccacc
     8641 gccttcatgc tcatcttcat catccccacg ttcgccaaga tgttcaccgg gttcggcgcc
     8701 gagcttccgc tgccgaccaa gatcgtcatg gggctctcct cgttcctgcg ctcctactgg
     8761 tgggtgatgc tgctcggcgt cgtggggacg ttcgtgggca tccagcgcta ctaccgcacg
     8821 gagcggggcc acatcgcgat cgaccgcttc ctgttgcgga tcccggtgct cggggacgtg
     8881 atccggaagg gcgccgtggc gcgcttcacg cgcacgctgg gcaccctgat ctcctcgggc
     8941 gtgccgatcc tcaccggact cgagatcacg gcgcgcacgt cgggaaaccg ggtcatccag
     9001 gaggcgatca tggcggcccg cgcgagcatc cgcgagggcg agacgatcgc ggcacccctc
     9061 cgcgtctcga acgtcttccc gccgatggtc gtgcagatga tctcggtggg cgaggagacg
     9121 ggcgccctgg acgacatgct cacgcgcatc gccgacttct acgacgatga ggtggatacc
     9181 gccgtcgagg cgctcacctc gctgatcgag ccgatcatga tcgtgttcat gggcagcgtc
     9241 gtgggcggca tggtcatcgc catgtacctg ccgatgttca agctcatcaa cgtggtcgcg
     9301 agcgggcact aggagaccga tggagctcgc gcgccctccg ctcgcaccag ggccggacga
     9361 ggcgctgcgg cgcgaggtcc gcgccgtcat cgtcggccgg atcgccctga tcacgctggt
     9421 cacggccatg gcggccgcgt tctcggaccg gatcgccgag cccgcccaga acgtgccgct
     9481 cgggttcctg gtggcgctct cggcgggact ggcggtcgcg taccaccggg ccgcgaagag
     9541 cggggcgccg ctccggtctt tgctcgcgat gcagctgatc gtggacgtgg cggtgatcag
     9601 ctatctcctc ctcttcacgg gaggggcgtc gagcccgttc gtgccgctct atctcctggc
     9661 accgctcctg ggcggggtgt tcctgagcgt gcagggaggg atgctgctcg cggtcgcggg
     9721 ggcggtcgcg tacgcggtgc tctacgccgc cgagcgcggg gggatgctcc cgcccgtctc
     9781 gtacgggctg acggagcggc tcagcgaatc ggccctgaag cttcggctgg tcctgtactt
     9841 gccgctcctg ttcgtcgtgg gggcgatcgg cggggccctg gggcggaccc ttcgcgaggg
     9901 caagaaggcg ctggacgagg cccgagccga gctctcgcgg gcgctgttcg acaccgagac
     9961 gatcctcgag aacctctcga gcgggctcgc gagcgtggac gcccacggcg tggtgcggca
    10021 ctggaaccgg gccgcgggcg agatcctggg gcggccggcc tcggaggtgc gggggcgcca
    10081 ctacgagacg gcgctgggcg aggggttcga ggaattccac gcccgcatcc gcgagacgct
    10141 cgcgagcggc gtgcccgcgg cgcggcacga ggcgcgggtg acgacgcgcg gcgggcgcac
    10201 cgtcccgctc ggcatctcga cgagcgtgct cgtggacgcc gaaggcggcc ggcgcggcgt
    10261 catcgcgctc ttccaggacc tctcggatgt gaaggcgctg gaggaccgga tccgccgcgg
    10321 ggagaccctc gccgcgatcg gcgagctctc ggccgggatc gcgcacgaga tccggaactg
    10381 tctgaacccg atcgcgggat cggtcgaggt gctgcagcgg gagctgcagg tgacggggga
    10441 gaacgcgagg ctgctcgagc tgatcgtccg cgagtcggag cgcctcgaca acttcatccg
    10501 ggagctcctc gactacgcgc gcgagcgccc gctcaagacc gagcgggtcg acctggcggc
    10561 gctcacgcgg gagacggcgg aggttgcgcg gcggcacccg agcatggggg cggggaagac
    10621 agtgacggtg gaggcgccgg agaagccggt gtgggcccag gtggacttcg agcagacgaa
    10681 gcaggtgctg ctgaacctgg tcatcaacgc cctcgaggcc atcgagggcg cgggcagcgt
    10741 cacggcgcgc gtcgacccgg ccaacggatc gcgcagccgc gggcgcgcgg gcttcgcggc
    10801 gatcgaggtg cgggacacgg gcgcggggat ccgcgccgac gacctgcgcc aggtgttcga
    10861 gccgttcttt tcgaccaagc tgggcggcac cgggctcggg ctcgcgatcg cgaaccggat
    10921 cgtggagcgg cacggcggtg cggtggagat cgagagccgg gtcggcgtgg gcaccacgct
    10981 ccggctgtgg ctaccccgag cggagcgccc ggcgaagacc gtggcgcggg ccgcctgatc
    11041 aacccctaga gcgaggaagg tcctgtggcc ggcgagaaga tcctcatcgt agacgacgaa
    11101 gagagcatgg tgcagttcct ctcgatcctc ctcaccaagg aagggtacga cgtccacgtg
    11161 gctcgctcgg ggaaggatgc cctggaacag gcggagggcg agaacttcga cgtggtcgtg
    11221 accgacatca agatgccggg cgagatcgac gggctcggcg tgctgtcggg ggtcaagaag
    11281 atcgatccct cggtcccggt cgtgatccgc accgccttcg cctcgcagaa gtcggcgatc
    11341 gaggcgctga accgcggcgc ctaccagtat ctggagaaga gcgccaagaa cgacgagatc
    11401 cggctggtca tcaagaacgc gctggccatg cggcgggtcc agtcggagaa cgtgctgctg
    11461 aagcggcag
//
LOCUS       DASSLZ010000191         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_72161, whole genome shotgun sequence.
ACCESSION   DASSLZ010000191 DASSLZ010000000
VERSION     DASSLZ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72161"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3305)
                     /locus_tag="VFM00_07510"
     CDS             complement(<1..3305)
                     /locus_tag="VFM00_07510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7498132.1"
                     /translation="MFPRITETFVLNEVLALRRRGVPLRIYSLLPPVRDVRIHPEAEA
                     LLDEVWVVSPLSWRSAVELGSALKRLFQTHPFRTASYLLGGIIHLSPGGLRNGIRGLL
                     LADRLLRDDVAHVHAAWAHTPATVTEAASRLTGIPWSMAAHAKDIFTSRPESLARRMG
                     SARFTLACSTAHAEHLRTLAARHGPPGRVPTVILAHHGVDADFFAPGASPVARNGVPR
                     IVSIGRLVPKKGMDQLVLAAARLRDRGVSFHLDIVGEGPLRGILEREVRERGLTNEVR
                     LTGLLVREEVREALRGATCFALACRVDESGDRDGVPNAIAEAMACGTPVVATRLPGIE
                     ELVADGCGTLVAPGDVEALGDALESMIREPGARDLVGARARARILEVFDARRCEAERT
                     RILAQSLSVRRILYVSADRGVPVGGVKGASVHVRSLVRALHAKEVESAVVTTNAGRGG
                     SGGSPIPFRVVEARAQGTWASAARAMAALTPFRSRDANARAALERAFLRLADNVALFR
                     SASDVTGAWSPDAVYERYSLCAVAGSVLARRLAVPHVLEMNAPLADEEARHRGLALPG
                     LTRAVERWILRRADRVLVVSEALRVHAEQLGAAPDRIRVVPNGVDPELFRPDRPGTTV
                     RNGSSPEDAVWVGFSGSLKPWHGTEHLVRALATASREDPRLRLLVLGEGPERGRLREL
                     ACSLAIEDRIEFAGAVAHERVPEYLASCDILSAPYGPTDDFYFSPLKIAEYRAAGKPV
                     VASRVGDLARTLDEAAGVVLLEPGDEQALARTLVDLARDPETRARLGRAAAAQSWTWS
                     HVADATLEAIDAARPACWRWATRRPLTIGYVVKMFPRFSETFIVNEILEVERLGVNVV
                     VFSMKENDETIRQPAVARVRAPVRVLPAAVSRFDQFGTHAAMLLRHPIRYGRTFLYVR
                     SRGSESAWSKFRIAPWLAREAHARGVHHLHAHFASGPARQAKFASMLSGIPFSFTAHA
                     KDLYWSGHRHEDRHKLKKRLQLASFVVAISHQNRRFLENRGFHVPRRKVVTIHNGIDL
                     AIWTFRRPQGRPVSPCPGDPVMARDETPTVLAVGRLVEKKGFGVLIDAMAVLLHRGMR
                     ARAWIVGDGPERGR"
BASE COUNT          568 a         1138 c         1189 g          466 t
ORIGIN      
        1 cgcccgcgct ccggtccgtc gccgacgatc cacgcgcgcg cccgcattcc gcgatgaagc
       61 aggaccgcca tcgcgtcgat cagcacgccg aaccctttct tctccaccag ccgcccgacg
      121 gcgagcacgg tcggagtctc atcacgggcc atgacggggt cacccgggca aggcgagacc
      181 ggccggcctt gggggcgtcg gaacgtccag atcgcgaggt cgattccgtt gtgaatggtg
      241 accactttcc ggcgcgggac gtggaatccg cgattctcca ggaatcggcg gttctgatgg
      301 ctgatcgcga cgacgaagga ggcgagttga aggcgtttct tcagcttgtg ccggtcctcg
      361 tgtcgatggc ccgaccagta gagatccttg gcgtgcgccg tgaagctgaa cgggatcccg
      421 gagagcatcg acgcgaactt ggcctggcgc gccggcccgc tcgcaaaatg ggcatgaagg
      481 tgatgcacac cccgggcgtg agcttcccgt gccaaccagg gcgcgatgcg gaatttgctc
      541 caggcgctct cgctcccgcg actccgcacg tagagaaacg tacgcccgta gcgaatcggg
      601 tgacggagga gcatggctgc gtgggtgcca aactgatcga agcgagaaac ggccgcgggc
      661 agcacgcgca ccggcgcgcg cacgcgcgcg acggcgggct gccggatcgt ctcgtcgttc
      721 tccttcatgg agaacaccac gacgttcacg ccgagccgct cgacctcgag aatctcgttc
      781 acgatgaacg tttccgaaaa gcgggggaac atcttcacca catagccgat ggtaagcggc
      841 cgtctcgttg cccatcgcca gcacgcggga cgcgcggcgt cgatcgcctc caaagtcgcg
      901 tcggcgacgt gggaccaggt ccaggattgt gcggcggccg cgcggccgag cctggcacgc
      961 gtttcgggat cacgggcgag gtccacgagc gttcgcgcca acgcttgctc gtcgccgggc
     1021 tccaggagca cgaccccagc cgcctcgtcc aaagtccggg cgagatcgcc tacgcgcgaa
     1081 gcgaccaccg gcttgccggc ggcccggtac tcggcaatct tgagcggtga gaagtagaag
     1141 tcgtccgtcg gaccgtacgg cgcggagagg atgtcgcagg aggccagata ctcgggcacg
     1201 cgttcgtgcg cgacggcgcc tgcgaattcg atacggtcct cgatcgcgag ggagcaggcg
     1261 agctcgcgca gacgccctcg ttccggaccc tcgccgagca cgaggaggcg aagtctggga
     1321 tcctcgcgtg aggcggtcgc gagcgcgcgc acgagatgct cggtaccgtg ccagggcttg
     1381 agactgccgg agaacccgac ccatacggcg tcttcgggag aagacccgtt ccgcacggtc
     1441 gtgccggggc ggtccgggcg gaagagctcc ggatcgacgc cgttcgggac gacccggatc
     1501 cggtcgggcg ccgcgccgag ctgctcggcg tgaacccgaa gcgcttccga cacgacgagc
     1561 acgcggtcgg cgcgtcggag gatccatcgt tccacagcgc gcgtgagccc cgggagagcg
     1621 agaccccggt ggcgcgcctc ctcgtccgcc aagggggcgt tcatctcgag gacgtgcggc
     1681 acggcgaggc ggcgcgcgag gaccgagccc gcgaccgcgc acaggctgta gcgctcgtag
     1741 acggcgtcgg gcgaccaggc gcccgtgaca tcggatgcag agcggaagag cgcgacattg
     1801 tcagcgaggc gcaggaacgc gcgctcgagc gcggcgcgcg cgttcgcgtc gcgggaccga
     1861 aacggcgtga gggccgccat cgcgcgcgcg gccgaagccc atgtcccctg cgcgcgcgcc
     1921 tcgacgaccc ggaaggggat gggcgatccg cccgatcctc cgcgtccggc attcgtcgtc
     1981 acgaccgctg actcgacctc tttggcgtgc agagcccgga ccagggatcg gacgtggacc
     2041 gaggctccct tcactccgcc caccgggacg ccacggtccg cggaaacgta gaggatccgt
     2101 cgaaccgaaa ggctctgggc gaggattcga gttcgctcgg cctcgcaccg tcgtgcgtcg
     2161 aagacctcga ggatgcgcgc gcgggcgcga gcgcccacga ggtcacgggc gccgggctcg
     2221 cggatcatcg actcgagggc gtcccccagc gcctccacat cgccgggcgc aaccaacgtg
     2281 ccgcagccgt ccgccacgag ctcttcgatc ccaggaagac gtgtcgccac gaccggcgtg
     2341 ccgcacgcca tcgcctcggc gatggcattg gggacgccgt cccgatcgcc gctctcgtcc
     2401 acacgacagg cgagcgcgaa gcaggtggcg ccgcgcagcg cctcgcgcac ctcttcccga
     2461 accaggaggc cggtgaggcg gacctcgttc gtcaggcctc gctcgcggac ctcgcgctcc
     2521 aggatcccgc gaaggggccc ctcgccgacg atgtcgagat ggaacgagac gccgcggtcc
     2581 cgcagccgtg ccgccgcgag cacgagctgg tccatgccct tcttcggcac cagccggccg
     2641 atgctgacga tgcggggaac gccatttcga gccacgggag aagcaccggg agcaaagaaa
     2701 tcggcgtcga cgccatggtg cgccaggatc acggtaggga cgcgccctgg cggcccatgg
     2761 cgcgcggcga gggtccggag atgctcggcg tgagcggtac tgcaggcgag cgtgaatcgc
     2821 gcgcttccca tgcgacgcgc gagtgattcc ggccgggacg tgaagatgtc cttcgcgtgc
     2881 gccgccatgc tccaagggat tccggtcagc cgggaggctg cctccgtcac agtggccggc
     2941 gtatgcgccc aggcggcgtg gacgtgtgcc acgtcgtcgc ggaggagccg gtcggccaga
     3001 aggagccccc ggatcccatt tcgaagcccg ccaggagaca gatggatgat gcccccgagc
     3061 agataggagg cggtgcggaa cgggtgggtc tggaacagac gcttcagggc gctcccgagc
     3121 tcgacggcgc tgcgccagga aagaggggaa acgacccaca cctcgtcgag cagcgcctcc
     3181 gcctcggggt gtattcgcac gtcccggacg ggaggcagca aggagtagat ccgtagcggc
     3241 acgccccggc gccgcagggc caggacctcg ttcagcacga aggtctcggt aatgcgcggg
     3301 aacatcttcg ccacataggc cagccttcct tcagtatggc tggcggctgc gcatcgatcg
     3361 t
//
LOCUS       DASSLZ010000192         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_704884, whole genome shotgun sequence.
ACCESSION   DASSLZ010000192 DASSLZ010000000
VERSION     DASSLZ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704884"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFM00_07515"
     CDS             complement(<1..706)
                     /locus_tag="VFM00_07515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7498133.1"
                     /translation="MSGPGTAAGREGARLRIVSYTTLFPNPAEPTRGIFVRNRLAGVA
                     AHADLVVIAPVNAGRNPRVLRTPLRRKDPAGFDVLHPRFAVLPGLLKGWDAALLHAET
                     LPQIRGALDAALGGARPDLVDAHYAYPDGAAAARLARHLGAPFVLTVRGSDLEVLAQS
                     ASQRGPIQRTLREARAVVAVSRSLERRAIELGAARDHVHVVPNGVDVGRFRPLDRSAS
                     REGLGLPAEGRVLLAVA"
     gene            complement(703..2592)
                     /gene="asnB"
                     /locus_tag="VFM00_07520"
     CDS             complement(703..2592)
                     /gene="asnB"
                     /locus_tag="VFM00_07520"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET7498134.1"
                     /translation="MCGICGLFAPGAGAVDRAVVERMTSSIVHRGPDDVGVHVKGPIG
                     LGFRRLSIIGIETGHQPMFTEDGRLAIIFNGEIYNYRELRRELEGLGHRFRTETDTET
                     ILLAYRQWGPDSVRRLRGMFGYAIWDEAEGALFLARDRLGIKPLYYAWDGTTLRFGSE
                     IKAILEDRSFKPTLDPLALDEYLTYLYVPAPRSIFREVRKLRPGHTLTATARGIEERE
                     YWDLAIRPGPARSEEEVVEGLRDALRDSVACHLMSEVPLGAFLSGGIDSSAVVAVMAE
                     LDDRPVRTESIGFREDAYDELPFARQVARAFGTDAHEKVVEAHAADILDALAWHYDEP
                     FADSSMVPTWYVSKAARERVTVCLSGDGGDENFAGYRRMRFDYLENRIRSRIPRWVRR
                     GVLAPVAAAYPKADRLPQVFRGKTLLTNLTLSPERAYHNTMRWFTPAMKARLYRPALR
                     AQVGDHDAFGVQEEFFRRSEGWHPLSRIQYVDFKTYLPDDILAKVDRASMAHSLEVRV
                     PLLDHRFVEHVATIPADLKIRNGSGKYVFKRALEGLVPSDVLTRPKMGFSVPLDLWFR
                     GELRAPFEERVLAPGSFTESLFEPGAMRSLWDEHQKGTRDRSYPLWALLVLEHWGRRF
                     APAAA"
     gene            complement(2611..>3266)
                     /locus_tag="VFM00_07525"
     CDS             complement(2611..>3266)
                     /locus_tag="VFM00_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HET7498135.1"
                     /translation="YAAAHHSVLTGGYDLGLYPYIKSWLYAPAMRAEVGNGGGNGGAT
                     LPAGLTRADLEPLDLVSRLQWIDTVAYLPDDILTKVDRASMAHSLEARAPLLDYTLVD
                     FMARVPTGLKLREGVSKYLFRKMLAKRLPEAVFTKRKQGFAVPKGEWFRRDLKAVARE
                     RLLDPRTLARGYFRPERIREVLQLHEAGQRDYSDWIWCLLILEEWHRTFMDPATRRI"
BASE COUNT          496 a         1131 c         1189 g          450 t
ORIGIN      
        1 gcgccaccgc gaggaggacc cgcccctcgg cgggaagtcc cagcccctcg cgggaggcgc
       61 tccgatcgag cggacggaag cggccgacgt ccacgccgtt cggcacgacg tggacgtgat
      121 cgcgcgcggc gcccagctcg atcgcgcggc gctcgaggga ccgggagacg gcgaccacgg
      181 cccgtgcctc gcgcagggtg cgctggatcg gcccgcgctg cgatgcgctc tgggcgagca
      241 cctcgaggtc ggagccgcgc acggtgagca cgaacggggc gccgaggtgg cgcgccaggc
      301 gcgcggccgc cgcgccgtcg gggtaggcgt agtgcgcgtc cacgagatcg gggcgcgcac
      361 cgcccagcgc cgcgtcgagg gccccgcgga tctgcggcag cgtctcggcg tggagcaggg
      421 ccgcgtccca gcccttgagg aggccgggca gcaccgcgaa gcggggatgg agcacgtcga
      481 atcccgccgg gtccttacgg cggagcggcg tgcgcaggac gcgggggttt cgccccgcgt
      541 tcaccggcgc gatgacgacg agatcggcat gcgccgccac gcctgcgagc cggttccgca
      601 cgaagattcc ccgcgtcggc tcggccgggt tcggaaagag cgtcgtgtag gagacgatgc
      661 ggaggcgtgc gccctcgcgc cccgcggcgg tccccgggcc gctcacgccg cggccggcgc
      721 gaagcgccgc ccccagtgct cgagcaccag gagcgcccag agcggataac tgcggtcgcg
      781 ggtccccttc tggtgctcgt cccagagcga gcgcatcgcg cccggctcga agagcgactc
      841 ggtgaacgac ccgggggcga gcacgcgctc ctcgaacggc gcgcgcagct cgccgcggaa
      901 ccagagatcg agcggcaccg aaaaacccat cttcggccgc gtcagcacgt cggaggggac
      961 cagcccctcc agcgcgcgct tgaacacgta tttccccgac ccgttccgga tcttgaggtc
     1021 ggcggggatc gtcgcgacgt gctcgacgaa ccggtggtcg aggagcggca cccgcacctc
     1081 cagggagtgg gccatgctcg cgcggtccac cttggccagg atgtcgtccg gcaggtacgt
     1141 cttgaagtcg acgtactgga tgcgcgagag cggatgccac ccttccgagc ggcggaagaa
     1201 ctcctcctgc acgccgaagg cgtcgtgatc ccccacctgc gcccgcagcg cggggcggta
     1261 gaggcgcgcc ttcatcgccg gcgtgaacca gcgcatcgtg ttgtgatagg cgcgctcggg
     1321 cgagagcgtc aggttggtga ggagcgtctt gccgcggaag acctgcggca gccggtcggc
     1381 cttgggatag gcggccgcca ccggcgccag gacgccgcgc cgcacccagc gcgggatgcg
     1441 cgagcggatc cggttctcga ggtagtcgaa gcgcatgcgc cggtaccccg cgaagttctc
     1501 gtcccctccg tcgccggaga ggcagaccgt cacgcgctcc cgcgccgctt tcgagacgta
     1561 ccacgtgggg accatggagg agtcggcgaa cggctcgtcg tagtgccacg ccagcgcgtc
     1621 caggatgtcg gccgcgtgcg cctccacgac cttctcgtgg gcatcggtgc cgaaggcgcg
     1681 ggccacctgc cgcgcgaagg gcagctcgtc gtaggcgtcc tcgcggaagc cgatcgactc
     1741 cgtgcgcacg gggcggtcgt ccagctccgc catcacggcg acgaccgcgc tggaatcgat
     1801 cccaccggag aggaacgcgc cgagagggac ctcgctcatc aggtggcacg cgaccgaatc
     1861 gcgcagcgcg tcgcgcagtc cctcgacgac ctcctcttcg gaccgcgccg ggccggggcg
     1921 gatcgcgaga tcccagtact cgcgctcctc gatcccccgc gccgtcgccg tcagcgtgtg
     1981 cccggggcgg agcttccgca cttcgcggaa gatggagcgc ggcgccggga cgtacaggta
     2041 ggtcaggtac tcgtccagcg cgagcggatc cagcgtcggc ttgaaggacc gatcctcgag
     2101 gatcgccttg atctccgagc cgaagcggag cgtggttccg tcccaggcgt agtacagcgg
     2161 cttgatgccg agacggtcgc gcgcgaggaa gagggcgccc tcggcctcgt cccagatcgc
     2221 gtagccgaac atgccgcgca ggcgccgaac ggagtcgggg ccccactggc gataggcgag
     2281 gaggatcgtc tcggtgtcgg tctcggtgcg gaaccggtgc cccaagccct ccagctcgcg
     2341 ccgcagctcc cggtagttgt agatctcgcc gttgaagatg atcgccagcc ggccgtcctc
     2401 ggtgaacatc ggctggtgcc ccgtttcgat cccgatgatc gagaggcggc ggaagccgag
     2461 gccgatgggc cccttcacgt ggacgccgac gtcgtcggga ccccggtgga cgatcgacga
     2521 ggtcatgcgc tcgacgaccg cgcggtccac cgcgccggcg ccgggtgcga agaggccgca
     2581 gataccgcac atctcaggca gtctccacgt tcagatccgc ctcgtcgcgg ggtccatgaa
     2641 ggtccggtgc cactcctcga ggatcaggag gcaccagatc caatcgctgt agtccctttg
     2701 acctgcctcg tgcagttgaa gcacttcgcg aatccgttcg gggcggaaat agccgcgcgc
     2761 gagcgtgcgg gggtcgagga ggcgctcgcg ggcgaccgcc ttcagatcgc gccggaacca
     2821 ctcccccttg gggacggcga agccctgttt ccgcttggtg aagacggcct ccgggagccg
     2881 cttggcgagc atcttccgga acaggtactt ggagacgccc tctcgaagct tgagcccggt
     2941 gggcacgcgg gccatgaaat ccaccagcgt gtagtcgagg agtggcgcgc gcgcttccag
     3001 cgagtgcgcc atgctcgcgc ggtccacctt ggtcaggatg tcgtcgggga ggtaggcgac
     3061 cgtgtcgatc cactggagcc ggctcaccag atcgagcggc tcgaggtccg cccgggtgag
     3121 cccggccgga agggtcgcgc ccccgttccc gccaccgttc cccacttcgg cgcgcatcgc
     3181 cggcgcgtag agccaggact tgatgtaggg gtagagcccg aggtcgtaac cgcccgtcag
     3241 cacggaatga tgcgcggcgg cgtaga
//
LOCUS       DASSLZ010000193         7045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_327032, whole genome shotgun sequence.
ACCESSION   DASSLZ010000193 DASSLZ010000000
VERSION     DASSLZ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327032"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFM00_07530"
     CDS             <1..214
                     /locus_tag="VFM00_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498136.1"
                     /translation="DAPAAWTASLAPSGAETLVSLAGSGIAASGRAWVRMGGFRLEAL
                     VSDAAAPEGGGSSAGSLRIEWGREDQ"
     gene            269..1429
                     /locus_tag="VFM00_07535"
     CDS             269..1429
                     /locus_tag="VFM00_07535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7498137.1"
                     /translation="MSQLGTETAFEVLAKARALEAKGRSVVHLEIGEPDFDTPAPIRA
                     AAVRALEEGFTHYGPSAGLPEVREAIAAYIARDRKLTVAADQVVVTPGGKPVLTFAVL
                     ACVNPGDEVIVPNPGFPIYESAVRFAGGTPVSLALREERGFRFGAADVEALITPRTRM
                     LILNSPHNPTGSVLGPSDLDQIADLVKGKDIWVLSDEIYSKILYDGVHESIATRPGMQ
                     ERTILLDGFSKTYAMTGWRLGYGVMNATLAKHVARLATNVYSCATSFVQRVAPTALGG
                     PQDEVKAMVAEFRRRRDAIVTGLNEIPGVTCAKPSGAFYVFPNVKGLGMKSAEVEQAL
                     LEEAGVAALSGTAFGSFGEGYLRFSYANSIENIREGLKRFGAFVESHSKAAR"
     gene            1469..2425
                     /gene="arcC"
                     /locus_tag="VFM00_07540"
     CDS             1469..2425
                     /gene="arcC"
                     /locus_tag="VFM00_07540"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET7498138.1"
                     /translation="MSAPARRVFITLGGNAILPARGTGTFEEQCAITRATMQPIAALI
                     RDGARVVLSHGNGPIVGNILIRNEASRDQIPPMPLDVCGADSQGGIGYMMQQLLQNEL
                     KRLGLGDRTVVTAVTQVLVDERDPAFRRPTKPIGPFYTQERARLLAREKGWTVVEDSG
                     RGYRRVVASPRPLEVIEIGAIRELVDSGAVVIAAGGGGIPVTRQWDGALHGIEAVIDK
                     DLAGSLLARLLGCEQLVIITGIDRVALHYGRPEQRDLAEATADEMARWMDEGHFPPGS
                     MGPKIQSAIEFLRGGGREVVITSPGNLIEALAGRQGTRIRRD"
     gene            2489..4018
                     /locus_tag="VFM00_07545"
     CDS             2489..4018
                     /locus_tag="VFM00_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7498139.1"
                     /translation="MGGKEATLAGRDAGIAVRDLSLPSDGRWAIRALTFAARAGALHL
                     ILGERDTGKTALLETIAGLRAPAKGAVLVGGDESSPAARRRALALIPRETPVRQLRVL
                     ERLLLHDTPRAAKVGIQWKQGREEARALAARVGLEALLEVPYEALRPVERKLIEVAAA
                     VRQHPAALLLDEPTADLGPHEARHFLATLHRILGEEKIPGVLATTWPRDAFPEGSAVT
                     ILWRNTDPATLPLEGMTESALVERWTGGLDIRRAPSGPHAPGDVLLRVEGLILHGRGR
                     ETSLAGVDFDAAAGEVLAIVGAPADGLDLLHDALLGNRRPDQGSIHFLGKEIARAPRR
                     VRVESGMTFVNPPIARDQSVDEFTVEENLILGQTHVPPFARKGFLRPESVRGNCLRAL
                     EDFEIVGATPRSRLRDLSLGQRQRLIVAREVIHNPRLLVVRSPGQGLSLEAQEYVRRT
                     LILQCERGSGLLWLTDEPEEAMRVADRLAVLTLGKFVWLPVTETLTREAIIAEMSGAA
                     A"
     gene            4015..5067
                     /locus_tag="VFM00_07550"
     CDS             4015..5067
                     /locus_tag="VFM00_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498140.1"
                     /translation="MIPGRCRAGKREILANALVLLALIGGTHALGVGVLDLNPSRWTA
                     PWTGDVVLVGAGLLFLGVAFLMPLHGGMINLGIHAEFLAGYSVGAIIVRGGVFGPGLQ
                     AGLGLVAGAAVGALVGFGIAALRQKFAVHEILSGLLGAALFVPLARALSVDRMAPPAL
                     AIDLPALTQPLKWAPGLSLAPGVVLAWGILLLALSMVVAVLFAHFLRATVSGFEVRAS
                     GANPLAAAAIGTDVEGIQRSMMALGGACAGLTGALQLWTSPAVALERWPFPLGFAGIA
                     VALLGLGSLRGAAIAAFVLAAWLNTPYVAQVLDHPGYGAGAAALLLLPALWILPRTFP
                     DFGAPRAVWRTRFREE"
     gene            5403..6065
                     /locus_tag="VFM00_07555"
     CDS             5403..6065
                     /locus_tag="VFM00_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET7498141.1"
                     /translation="MLRTWVLLLWLLAATIAASLVVRLVPFARAEIGGDGLRLSEILA
                     GPARDWDGDGVYDSRGDEWVEVLNHGAAPVDLAPYRIADADSTIRYELSGTLLPGEAR
                     LVTGSAAVAQQRSLGRTASGLSLNNSGDTVILFRIGATDTTLVDAHRYGSIEGASDRS
                     TGWIDDPAAWVLFDGLNKYSGAGEPQGTGCSPTPGARNGCPLPVTETTWGKIKSIYSS
                     GR"
     gene            complement(6295..6870)
                     /locus_tag="VFM00_07560"
     CDS             complement(6295..6870)
                     /locus_tag="VFM00_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498142.1"
                     /translation="MNGRDRHRQLAFGTRRAALGVALALLTLAAPGCGRYDIQGDGQG
                     RIVRLDRLTGAVDYLQGSRFVRIENPGDHRREAEELARVHDWGALTYDNKYTLRLKTA
                     FRNDRLYYQIGIVPVPPANVFNFRIALNDADGFQVASLSLNTPDGVPTADSGVAYSGF
                     TQSDAEVYRSVRSWTPRIELYNGRIAPAELP"
BASE COUNT          973 a         2392 c         2587 g         1093 t
ORIGIN      
        1 cgacgcgccg gccgcctgga ccgcgtcgct tgcgccctcc ggggccgaga cgctcgtgtc
       61 gctcgcgggt tccggcatcg ccgcttcggg ccgggcctgg gttcggatgg gtggatttcg
      121 actggaagcg ctcgtctccg acgccgcggc gccggaggga gggggatcgt ccgcgggctc
      181 gctccgcatc gagtgggggc gggaagacca atagaatcgt ccagagagct ctggaggatc
      241 gggggaccgt atgcgttacg cggatcggat gagccagctg ggaacggaga ccgctttcga
      301 ggtgctcgcc aaagcgcggg cgctcgaggc caaggggcgc tccgtcgtgc atctcgagat
      361 cggagagccg gatttcgaca cgccggcgcc gatccgcgcg gcggcggtgc gcgcgctcga
      421 ggaggggttc acccactacg gcccttcggc cggcctgccc gaggtgcgcg aggcgatcgc
      481 ggcctacatc gcgcgcgacc gcaagctgac ggtggccgcg gaccaggtcg tggtgacgcc
      541 gggcggaaag ccggtgctca cgttcgcggt gctcgcctgc gtcaatccgg gcgatgaggt
      601 gatcgttccc aatccgggct tcccgatcta cgagtcggcg gtgcgcttcg ccggcggcac
      661 gcccgtgtca ctcgcgctgc gcgaggagcg cggcttccgc ttcggcgcgg ccgacgtgga
      721 ggcgctgatc acgccgcgca ccaggatgct gatcctgaac tcgccccaca atccgacggg
      781 atcggttctc ggcccgtccg acctggatca gatcgcggac ctcgtgaagg gcaaggacat
      841 ctgggtcctc tcggacgaga tctactcgaa gatcctctac gacggcgtgc acgagtcgat
      901 cgcgacgcgc cccggcatgc aggagcgcac gatcctcctc gacggcttct ccaagaccta
      961 tgccatgacc ggctggcggc tgggctacgg cgtcatgaac gccacgctcg ccaagcacgt
     1021 cgcgcggctg gccacgaacg tctattcgtg cgccacgtcg ttcgtccagc gcgtggcgcc
     1081 gacggcgctg ggcgggccgc aggacgaggt gaaggcgatg gtcgcggagt tccgccgccg
     1141 gcgcgacgcg atcgtgaccg ggctgaacga gattccgggc gtcacctgcg ccaagccctc
     1201 gggggcgttc tacgtgttcc cgaacgtgaa ggggctgggc atgaaatccg ccgaggtgga
     1261 gcaggcgctc ctggaggagg cgggtgtcgc cgcgctctcg ggaacggcct tcggctcgtt
     1321 cggcgagggg tacctgcgct tctcctacgc caactccatc gagaacatcc gggaaggatt
     1381 gaagcgcttc ggcgccttcg tcgagagcca cagcaaggcc gcgcgatgaa tcgatgagcg
     1441 cgacctcgtc cggcggctcg gcgattccat gagcgctccg gcgcggcgcg tcttcatcac
     1501 gctcggcggc aacgcgatcc tccccgcccg cgggacggga acgttcgagg agcagtgcgc
     1561 gatcacgcgc gccacgatgc agccgatcgc ggcgctgatc cgggacggcg cgcgcgtggt
     1621 gctctcgcac ggcaacggac ccatcgtcgg gaacatcctg atccggaacg aggcttcgcg
     1681 cgaccagatc cccccgatgc cgctcgacgt gtgcggcgcc gactcgcagg gcggcatcgg
     1741 ctacatgatg cagcagctgc tccagaacga gctgaagcgg ctcggcctgg gggatcggac
     1801 cgtcgtcacc gcggtcacgc aggtgctggt ggacgagcgc gatccggcgt tccgccgccc
     1861 caccaagccg atcggtccct tctacaccca ggagcgggcc cgcctgttgg cgcgcgagaa
     1921 ggggtggacg gtggtggagg actcgggtcg cggctaccgc cgcgtcgtcg cctcgccgcg
     1981 gcccctcgag gtgatcgaga tcggcgccat ccgcgagctg gtcgacagcg gcgccgtggt
     2041 gatcgcggcc gggggcggcg ggatcccggt cacccggcag tgggatgggg ccctccacgg
     2101 catcgaggcc gtgatcgaca aggatcttgc gggcagcctg ctcgcgcgct tgctcggctg
     2161 cgagcaattg gtgattatca ccggcatcga ccgggtggcg ctgcactacg gccggccgga
     2221 gcagcgcgat ctcgccgagg cgaccgccga cgagatggcc cgctggatgg acgaggggca
     2281 ttttcctccg ggaagcatgg ggccgaagat ccagagcgcg atcgaatttc tccgcggcgg
     2341 cggtcgtgag gtggtgatca cgtcgccggg gaacctgatc gaggcgctgg cgggaagaca
     2401 ggggacgcgc atccggcggg attgaaggca cagccaagcg ccggtcggaa cggcggccgt
     2461 acgggaagac ggccgagggg gagggaggat gggcggcaag gaggcgacgc tcgcgggacg
     2521 cgatgcgggg attgcggtgc gcgatctctc gctgccgtcg gacgggcgct gggcgatccg
     2581 cgcgttgacc tttgccgcgc gcgcgggggc gctccacctg atcctgggcg agcgcgacac
     2641 cggaaagacc gcgctcctcg agacgatcgc gggccttcgc gccccggcga agggcgcggt
     2701 gctcgtgggc ggggacgaga gctccccggc cgcgcggcgc cgcgcgctgg ccctgattcc
     2761 gcgcgaaacc ccggtccgcc agcttcgggt cctcgaacgc ctactcctcc acgacacccc
     2821 gcgggccgcg aaggtcggaa ttcagtggaa gcaggggcgt gaggaagcgc gtgccctggc
     2881 cgcgcgcgtg ggcctggaag cgctcctcga ggtgccctac gaagcgctcc gccccgtgga
     2941 gcggaagctg atcgaggtgg cggccgccgt gcgccagcac ccggccgcgc tgctcctcga
     3001 cgagccgacc gcggacctgg gcccgcacga agcccgccac tttctcgcca ccctccatcg
     3061 aatcctgggc gaagagaaga tcccgggcgt gctcgccacg acctggcccc gcgacgcctt
     3121 tcccgagggc tcggccgtca ccatcctgtg gcggaacacc gatcccgcga cgctgccgct
     3181 ggaagggatg accgaatcgg cgctggtgga gcgctggacc ggcggcctgg acatccgccg
     3241 cgcgcccagc gggccgcacg cgcccggcga cgtgctgctt cgggtcgagg ggttgatcct
     3301 gcacggccgc ggccgcgaga cgtcgctcgc cggcgtggac ttcgacgcgg cggcgggcga
     3361 ggtgctcgcg atcgtgggcg ctccggccga cggactcgac ctcctccacg acgcgctcct
     3421 cggaaaccgg cgccccgacc agggctcgat ccatttcctc gggaaggaga tcgccagagc
     3481 gccgcgccgc gtgcgcgtcg agtcggggat gaccttcgtg aacccgccga tcgcgcggga
     3541 tcagtcggtg gacgagttca ccgtcgagga gaacctcatc ctgggccaga cgcacgtgcc
     3601 ccccttcgcg cggaagggat tcctccggcc ggagtcggtc cgggggaact gcctccgcgc
     3661 gctcgaggac ttcgagatcg tgggcgccac gccccggtcg cgcctgcgcg acctgagcct
     3721 ggggcagcgc cagcggctga tcgtcgcgcg cgaggtgatc cacaacccgc ggctcctggt
     3781 cgtgcggtcg ccggggcagg gtctctcgct ggaggcgcag gaatacgtgc gccgcacgct
     3841 cattctccaa tgcgagcggg ggagcggcct gttgtggctg accgacgagc ccgaggaggc
     3901 gatgcgcgtg gccgaccggc tcgccgtcct cacgctgggc aagttcgtgt ggctgccggt
     3961 gacggagacg ctcacgcgcg aagcgatcat cgcggagatg tcgggagcgg cggcgtgatt
     4021 ccgggccgct gccgcgcggg caaacgcgag atcctggcca acgcgctcgt cctgctcgcc
     4081 ttgatcggcg ggacgcacgc gctcggcgtc ggcgtgctcg acctgaaccc ctcgcgctgg
     4141 accgcgccct ggaccggcga cgtcgtgctc gtgggcgcgg gtctcctctt cctgggtgtc
     4201 gccttcctca tgccgctgca cggcggcatg atcaatctcg gcatccacgc cgagttcctc
     4261 gcgggctact cggtgggggc gatcatcgtc cgcggcggcg tgttcggtcc cgggctgcag
     4321 gcgggactgg gcctggtggc cggcgcggcc gtgggggcgc tggtcggctt cgggatcgcg
     4381 gcgctgcgcc agaagttcgc cgtgcacgag atcctgagcg gactcctggg ggcggcgctc
     4441 ttcgtgccgc tggcccgggc gctgtcggtg gaccggatgg caccgcccgc gctggcgatc
     4501 gacctgcccg cgctgaccca gccgctgaag tgggcgccgg ggctttcgct cgctcccggg
     4561 gtggtgctcg cctggggcat cctgctgctc gccctctcca tggtcgtggc ggtgctcttc
     4621 gcgcatttcc tgcgcgccac ggtcagcggc ttcgaggtgc gcgcctccgg agccaatccg
     4681 ctcgccgccg ctgccatcgg caccgacgtc gaggggatcc agcgctcgat gatggcgctg
     4741 ggcggcgcgt gcgcgggttt gacgggcgcg ctccagctct ggacctctcc cgcggtggcg
     4801 ctcgagcgct ggccgttccc gctgggcttc gcgggcatcg ccgtcgcgct gctcgggctg
     4861 ggctctctgc gcggggccgc catcgccgcg ttcgtgctcg cggcgtggct caacacgccc
     4921 tacgtggccc aggtgttgga ccatcccggc tacggcgccg gcgcggcggc gctcctgctc
     4981 ctccccgcgc tgtggattct cccgcggacg ttccccgact tcggcgcgcc gcgcgccgtg
     5041 tggcggacga ggttccgcga ggagtagcct cccatgcgcg tcgcgacggc catgggtctc
     5101 gcctggatgc tggcgctcgc gaccgcctcg tgttcggcgc cgatggcgtc gtgagcgatc
     5161 ccgcgaggtg gcgctcgatt gccgcggatc gaattcgcga cggagcgcgc cggctgctcc
     5221 gggagaacga gaagaaactg cgacggctgg gcgccgcgac cacggaacgc ttccttccgg
     5281 agtgaacgcc ccttgccgcg gttcgcggcg ggggtgtatc gtacctctct cctcgtcgaa
     5341 cgcgtagaat cccccggttc catccccgac gccaccgcgc catcggcgca cggaggatgg
     5401 tcatgcttcg aacgtgggta ctcctgctgt ggcttctggc cgcgacgatc gcggcgtccc
     5461 tggtcgttcg cctggtcccg ttcgcgaggg cagagatcgg tggggacggg cttcgtctct
     5521 ccgagatcct ggccggcccg gcgcgcgact gggacggcga cggcgtctac gactctcgcg
     5581 gcgacgagtg ggtcgaggtg ctcaaccacg gcgcggctcc ggtggacctg gcaccgtatc
     5641 ggatcgccga cgccgactcc accatccgct acgagctctc gggaacgctc ctgccgggcg
     5701 aagcgcggct ggtgacggga agcgcggccg tggcgcaaca gcgctccctg ggccggacgg
     5761 cgagcgggct ctcgctcaac aactcgggcg acacggtgat cctgtttcgg atcggcgcca
     5821 ccgacaccac gctggtggac gcgcaccgct acggaagcat cgagggcgcg agcgaccgct
     5881 ccacggggtg gatcgacgat cccgcggcgt gggtcctgtt cgacgggctc aacaaatact
     5941 cgggcgcggg cgagccgcag gggacggggt gcagtcccac gccgggggcg cggaacggat
     6001 gccctcttcc cgtgacggag accacgtggg ggaagatcaa gtccatctac tcgagtggcc
     6061 ggtgatgcta cttaggtata cctctccagg gtactaggaa actggtgttt cccaggaagt
     6121 ttgtgaaata gttcacaatg ttcgggcgga aagcgcggcg cggccgacgg cctttgggcg
     6181 atgcgcccca gcgtccgcgc taccgcccga acacctccta gggtttctga ctcgaaaccg
     6241 aaagcggcgg ccggaaggga cagccggccg cctcttcttt tggggcggga tggttcaggg
     6301 gagctcggcg ggagcgatgc ggccgttgta cagctcgatg cgcggggtcc acgaccggac
     6361 cgagcggtag acctcggcgt cggactgcgt gaatccggag taggcgactc cgctgtcggc
     6421 ggtcggcacg ccgtctggcg tattgagcga gaggctcgcg acctggaagc cgtccgcgtc
     6481 gttgagcgcg atccggaaat tgaagacgtt ggcgggtggg acgggaacga tcccgatctg
     6541 gtagtagagg cggtcgtttc gaaaggccgt cttcagccgg agcgtgtact tgttgtcgta
     6601 cgtcagcgct ccccagtcgt gcacgcgggc cagctcctcg gcctcgcgcc tgtggtcgcc
     6661 cgggttctcg atgcggacga agcgcgaccc ctgcaggtag tccactgcgc cggtcaagcg
     6721 atccagccgg acgatgcgcc cctggccgtc cccctggatg tcgtagcggc cgcacccggg
     6781 agccgcgagc gtgagcagcg cgagcgcgac cccaagcgcg gctcgccgcg tcccgaaggc
     6841 gagttgccgg tgcctgtcgc gtccgttcac gcggacgagt ttcgcatgga aacaggtact
     6901 taggtagcag tacctgtgta ccattccgtc agggccggca ccgtgttacg cttccgcctc
     6961 ggaagtcgcc cacctccccg aatcctcatc gtcccgagag cggtcgtacc gacaggagtc
     7021 tccgccatgt cgcgagtcgc gtcgc
//
LOCUS       DASSLZ010000194         6492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1069802, whole genome shotgun sequence.
ACCESSION   DASSLZ010000194 DASSLZ010000000
VERSION     DASSLZ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069802"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(531..1085)
                     /locus_tag="VFM00_07565"
     CDS             complement(531..1085)
                     /locus_tag="VFM00_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498143.1"
                     /translation="MTEYEGRPCLYLDGAEATVRDLEMRDGVIDVDVTTPASRGFFGI
                     ECRMASGDQNAEWVYLRQHRSEYPDAIQYTPVLNTGLNWQLYNGPGFTAAVEIPKNQW
                     FHLRLELTGAQAKFYVSDMTKPALVMDDLKSGVEKGRVGLAALTGATYFSNFDVRASP
                     DAPWGRHLPPMPPGTLTQWSLSPS"
     gene            complement(1357..3672)
                     /locus_tag="VFM00_07570"
     CDS             complement(1357..3672)
                     /locus_tag="VFM00_07570"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HET7498144.1"
                     /translation="MTFSTGALFSLLAGASWGDLLSLLVRVTLIAAAGGCLCALLRHA
                     SAAKRHLAATTTLTALIALPIAWALLPSVSLPILPAKPVPTSTVERPAPAPEVAGGSS
                     SVGFPRPHIGTVTGITAPASLHVSGSQAITLAILITSVVTAALLLHLFLSLAAAWITA
                     RRARRIEDASLRHDLDEARERLGVSRPVDLRESANVGVPVVWGFLRPVLLLPLGARHW
                     TREQLRVVFLHEVAHVARRDGMGLLLGRVVTSLFWFHPLVWMLARIARRECERCCDDL
                     VLAAGERPTDYAERLLAIVRSMTRPGTLAGVAPALAQRSNLEGRLVSILSAKQRRGPA
                     SRAGIVATIGSAAFLLVVTATVHVVAAHPVGRLVDPASETASIPAGDPSAAVHAILDR
                     EPTTPQSPEQSKVGEQSFRAGEEAFREGSYVRAVSSYLAAVAAGHRCPESLYRAAGAL
                     AKAGANEDAMRTLEAAIQAGFDGTEMASDPNLKDLRADPRFEALASPQPLPTPMAASV
                     PMPVVASVPPTVYRVAGSLPNDPSGIDLLRAGQYDRAVAAFEEEVRQTGSTNAMYNIA
                     CAYALHGDKRRAFDALERAIENGFDNSQVMIQDDDLRLLQGDPHFYQLVRLTKDLQLY
                     GSMPFGFRGPEDWRASLSRFERVTKEHPNLGRAWANLGFARLEAGDPKGGAAAYERAL
                     DLGYQSPTVMYNLACCAARSGDVDQAFQWLDRADKAGFEIGEHVGSDSDLDALRGDRR
                     YDDLLERWDQKMAKEHREKKQEKDKEGDKTY"
     gene            complement(3669..4061)
                     /locus_tag="VFM00_07575"
     CDS             complement(3669..4061)
                     /locus_tag="VFM00_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HET7498145.1"
                     /translation="MKRLKTLSRREREIMDIVYASEGASATAGEIQSRLSSPPSYSAV
                     RATLRILEQKGFLKHEEDGTRYVYKPVLTRDKARQGAIEHLLTTFFNGSAAGAVLTLL
                     EQPGTELTPQELDRMAKLIEQARNEERK"
     gene            4206..6059
                     /locus_tag="VFM00_07580"
     CDS             4206..6059
                     /locus_tag="VFM00_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498146.1"
                     /translation="MASLPRLKGGLQILLWIAIVVLGTYAFTRLRWRLEERYPLVKFE
                     QTREAQQFVSLRQYAAREQLGSFLPSHDAQIAIGERLLRDVLTRSLPIRQSFEHGRYE
                     ARLDRARLELEDGLASIRMFGQVRRVDPKRTIQADVELQTHIDVVEFRPDVGTIRAGL
                     SVTGAHVIRAGHGSAKTFMNPAARFFGGLKVEDWNRERLSLELPIHLDQEITIPDING
                     EVSLRARRIPLRVRVSALTVLQNRLVLSLFLERDADAGEPPPAEPARVVLTPRAREQM
                     EETARRLFQQGHGVAARDSILRRVQALAARDSLWQGILSSDRDVVVLVPRSLCQVLCN
                     RVARSYLQGVQLNLDPKVSVHLDEQIRVRALGGNVGAGKIVGDLRVTSLRGRLRVSED
                     PRLALVPPDGFEITAPVRVQEGQGRVRLDMRWDPSFLVSIVCRGFGFGETLSGEVLPF
                     SHVLRTRVRFQTRGSRFVGRPLVRSDRVSVPCEFTPASYTKVRTALLEQDRLLRCGLV
                     MDADTVLEKVRGLVRHSVRFTMPSTLFKPFGFPLFLEEEYTAGEFRIAALARKPEVIV
                     RPSYLRLGFNAELAVRSQEMIRPGGEAVPKLRATPPSTKAPRASRFPKGGP"
     gene            complement(6043..6318)
                     /locus_tag="VFM00_07585"
     CDS             complement(6043..6318)
                     /locus_tag="VFM00_07585"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498147.1"
                     /translation="MNDGDLGALHDPVRIRIYFLAALPLETRKRFLAEARAGLLRTLA
                     LMRMDLAEYERENSRLSALATRGAIELTRAQLRWLSTCGRVLVKDLP"
BASE COUNT         1047 a         2166 c         2194 g         1085 t
ORIGIN      
        1 tgaagtggga tccggtctcg agcaggagct tcaccgcccg ggcgcacgcg atcatctccg
       61 cctcgtgggg aacttcgact aggaatcgtt gcatcggtca ccctcctcgg aagtgcacgc
      121 tacgggtcac gatagcatcg cccgacaggc gtcggcaatc ggcagccgac acgccacgga
      181 cgacctcacg ctcatggccc caggctctcc agccggattc tgggcgctcc tcccccggta
      241 caggatgtgc ccgttcagga agacgctgac ttcgtcgcta tagccgatgc agagcttctt
      301 cacctcgtcg cggggcgacg ggatggtggt ccgggcatac acgacggccg tgccgggctg
      361 ggatccaggc gcttcgagaa gtcgttggcg aacgagaccc ggggatgcgg gctgtcgcga
      421 taccgattga tcgcgacgat gccaggcggc tcggcctgga cctcctgcca gcgcatcgtg
      481 tcgctctcgg ctcgcgacag cggtcgctcc agaccgcgtt ggagcgcgtc ctaggagggc
      541 gacaggctcc actgcgtcag ggtgccgggc ggcatcggcg ggaggtgccg cccccatggc
      601 gcgtccggcg aggcgcgaac gtcgaagttc gagaaatacg tcgcgccggt cagggcggcg
      661 aggcccacac gacccttctc cacgccgctc ttcaaatcgt ccatgacgag cgcgggcttg
      721 gtcatgtccg agacgtagaa tttcgcctgc gcgcccgtca gctccagacg gaggtgaaac
      781 cattggttct tcgggatctc gacagcggcc gtgaagccgg ggccgttgta caactgccag
      841 ttcaggccgg tgttgaggac cggagtgtac tggatggcat ccggatattc ggacctgtgc
      901 tgtcgaagat agacccactc ggcattctga tcgccgctcg ccatgcgaca ctcgatgccg
      961 aagaagccgc gactcgccgg agtcgtgaca tcgacgtcga tgaccccgtc gcgcatctcc
     1021 agatcccgca cggttgcttc cgctccgtcc aggtagaggc aagggcgccc ttcgtattcg
     1081 gtcacctcgg cctcgcctgc caacgaccac cggatggtgt ctggaggaac cgaaagcacc
     1141 cctgtcgtct cggaacatgc ggtgcgggct acgagaaggg ccatcctgct ggcgagcagg
     1201 aatcgaagca tgactgtcct ccgaagtgac cgatgtcggt gctggagcgg ccggcagacg
     1261 gcgtgatggg ggacaggatg gctagagcgt agcgacctgg cgttggttga agcgcctggg
     1321 ggctcccgtt tggaacctca aaggcttgac gcggaatcag tacgtcttgt ccccctcctt
     1381 atccttctcc tgcttcttct cccggtgctc cttcgccatc ttctggtccc accgctccag
     1441 caggtcgtcg taccgccggt cgcctcggag ggcgtccaga tcggagtccg agcccacgtg
     1501 ctcgccgatc tcgaagccgg ccttgtccgc acgatcgagc cactggaacg cctggtcgac
     1561 atcgcccgat cgagccgcgc agcaggcgag gttgtacatg accgtcgggc tttgatagcc
     1621 caggtcgagc gctcgctcat aggcggcggc gcctcccttg ggatcccccg cttccaggcg
     1681 ggcgaatccc agattggccc acgcgcgccc caggttggga tgctctttgg tgacacgctc
     1741 gaaccgcgac agcgaggcgc gccaatcctc cgggcctctg aacccgaacg gcatggaccc
     1801 gtagagctgc aggtccttgg tcaggcgaac gagctgatag aagtggggat cgccctggag
     1861 caggcgcaga tcgtcgtcct ggatcatgac ctggctgttg tcgaagccgt tctcgatggc
     1921 gcgttccagg gcgtcgaacg cgcgccgctt atcgccatgc agcgcgtagg cgcaggcgat
     1981 gttgtacatc gcgttcgtgc ttccggtctg tcgcacctct tcctcgaacg cggcgaccgc
     2041 ccggtcgtac tgacctgctc ggaggaggtc gatgcccgac ggatcgttcg gcaatgagcc
     2101 cgcgactcgg tacacggtgg gcggcacgct cgcgaccaca ggcataggca cgctcgccgc
     2161 catcggcgtc gggagcggct gcggggacgc gagtgcctcg aaccggggat cggctcggag
     2221 atccttgagg ttcgggtccg acgccatctc ggttccatcg aagcccgctt ggatggccgc
     2281 ctccagcgtc ctcatcgcgt cctcgttcgc gccggctttg gccaaggcgc cggcggcccg
     2341 gtacagcgat tccggacacc gatgacccgc cgcgaccgcg gccaggtacg acgagacggc
     2401 ccggacgtag cttccctccc ggaacgcctc ctcgcccgcg cggaagctct gctcgccgac
     2461 tttcgactgc tcgggcgact gcggcgtggt cggctctcgg tcgaggatcg cgtggaccgc
     2521 ggcgctcggg tcccctgccg gtatcgacgc cgtctccgac gccggatcga cgagccggcc
     2581 gacgggatga gccgccacga cgtggaccgt ggcggtcacg accaagagga atgccgcgga
     2641 accgatcgtc gcgacgatgc cggcacggga agcaggcccg cgacgttgct tcgccgaaag
     2701 gatcgagacc aggcggcctt cgagattcga ccgctgcgcc agggccggcg cgacccccgc
     2761 cagggtgccg gggcgcgtca tggagcgcac gatggcgagc aggcgctcgg cgtaatcggt
     2821 gggccgttcg ccggcggcga gcaccaggtc gtcacagcag cgctcgcact cgcgccgggc
     2881 gatcctcgcc agcatccaca cgagcgggtg gaaccagaac agcgaggtga cgacccggcc
     2941 gaggaggagc cccatgccgt cgcgccgggc tacgtgggcc acctcatgga gaaacacgac
     3001 gcgcagctgt tcccgcgtcc aatggcgggc tcccagcggc agaagcagca ccgggcgaag
     3061 gaatccccag acgacgggaa cgccaacgtt cgcgctttcg cggaggtcca ccgggcggga
     3121 cacgcccagc cgctcacgcg cctcgtccag gtcgtgtcgc agcgaggcgt cttcgatccg
     3181 tcgcgcgcgc cgagccgtga tccatgcggc agcgagcgaa aggaagagat gcaggagcag
     3241 cgccgccgtc acgaccgatg tgatcagaat cgcaagagtg atggcctggc ttccggagac
     3301 gtgcagcgag gcgggcgcgg tgattcccgt cacggttccg atgtggggcc gagggaagcc
     3361 gacgctgctc gagccccctg ccacctcggg cgccggagcc gggcgctcca cggtagacgt
     3421 cggcaccggc ttcgccggca ggatggggag ggaaacgctc gggaggagcg cccaggcgat
     3481 gggcagcgcg atcaaggcgg tcagcgtggt cgtcgcggcg agatggcgct tggccgcgga
     3541 ggcgtgtcgg aggagtgcgc agaggcagcc tccagcggcc gcgatcaggg tgactcgaac
     3601 gagcagggac agcagatcgc cccagctcgc gcccgcgaga agcgaaaaga gggctcccgt
     3661 cgaaaaggtc atttgcgttc ctcgtttctg gcctgctcga tcagcttggc catgcggtcc
     3721 agctcctggg gggtcagctc ggtgccgggc tgctcgagca gcgtcaggac ggcgccggcg
     3781 gcggaaccgt tgaagaaggt cgtgagcaga tgctcgatcg cgccctgcct ggccttgtcc
     3841 cgggtcagga cgggcttgta gacgtaacgg gtgccatcct cctcgtgttt cagaaatccc
     3901 ttctgctcca ggatgcgcag cgtggcgcgc accgcggagt agctgggagg cgacgagagg
     3961 cgagactgaa tctcgccggc ggtcgcgctc gctccctcgg acgcatagac gatgtccatg
     4021 atctcccgct cgcgacggct caaggtcttc aatcgcttca cgtgcccctc ccggtgctgg
     4081 gtttttagca gtgctgaatt attagcactg cgcgccgcgg gctgtcaata cgcaaagcga
     4141 tcgtgccgcc tcgagcggcg ccttccggtg caaccaccaa caagtcaaac gattacaatt
     4201 gcgggatggc gtccctgccg cggctgaaag gcggtctcca gattctcctg tggatcgcga
     4261 tcgtggtgct gggcacctac gcgttcacac gcctgcgctg gcggctcgaa gagcgctacc
     4321 ccctggtcaa gttcgagcag acgcgcgagg cgcagcagtt cgtcagcctg cgccagtacg
     4381 cggcgcgcga gcagctgggg tcgtttctcc cctcccacga tgcccagatc gcgatcgggg
     4441 aacgcctcct gcgcgacgtg ctcacgcgct ccctgccgat ccggcagtcg ttcgagcacg
     4501 gccgctacga agcgcggctc gaccgcgcgc gtctcgagct ggaggacggc ctggcgtcga
     4561 tccggatgtt cggtcaggtg cgtagggtcg atcccaagcg cacgatccag gccgacgtcg
     4621 agctgcagac ccacatcgac gtcgtggagt tccgccccga cgtggggacg attcgggcgg
     4681 ggctgagcgt cacgggcgcg cacgtgatcc gggcgggcca tggctccgca aagaccttca
     4741 tgaatcccgc ggcgcgattc ttcgggggac tcaaggtcga ggattggaat cgggagcgcc
     4801 tcagcctgga gctgccgatc catctcgacc aggagatcac gattcctgac attaacggcg
     4861 aagtgtcctt gagagcgcgc cgcatcccgc ttcgcgtccg ggtctcggcg ctgaccgtgc
     4921 tccagaacag gctcgtcctc agcctcttcc tggagcgcga cgcggacgcc ggcgaacccc
     4981 cacctgcgga gcccgcgcga gtcgtgctga cgccccgcgc ccgcgaacag atggaggaga
     5041 cggcccggcg tctcttccag caaggccatg gggtcgccgc tcgcgacagc atcctgcggc
     5101 gcgtgcaggc cctggcggcg cgcgactccc tgtggcaggg gatcctctcg tccgatcggg
     5161 acgtggtggt tctcgtgccc cgatcgctgt gccaggtgct ctgcaaccgc gtcgcccgca
     5221 gttatctcca aggcgtccag ctcaacctgg atccgaaggt ctccgtgcac ctggacgagc
     5281 agattcgcgt gcgggcactc ggggggaacg tcggcgcggg caagatcgtc ggcgacttgc
     5341 gggtcaccag tctgcggggg cgcctgcgcg tcagcgaaga tcctcgactc gcgctggtcc
     5401 cgcccgacgg gttcgagatc acagcgccgg ttcgtgttca ggagggacag ggccgggtgc
     5461 gcctcgacat gcgttgggat ccgagctttc tggtgagcat cgtctgccgc gggttcggtt
     5521 tcggagaaac cctttccggt gaagttctcc ccttctcgca cgttcttcgg acccgggtgc
     5581 gcttccagac ccgaggctcg cgattcgtcg gccgcccgct cgttcgctcc gatcgagtgt
     5641 cggtgccctg cgaattcaca ccggcgtcct acacgaaagt ccgcaccgcg ctcctcgagc
     5701 aggaccgatt gcttcgctgc ggcctggtca tggatgccga caccgtgctc gagaaagtgc
     5761 gcgggctcgt ccggcacagc gtgcgcttca cgatgccgag cacgctgttc aagccgttcg
     5821 gatttccgtt gttcctggaa gaggagtaca cggcgggaga gttccgcatc gccgccctcg
     5881 cccggaagcc ggaggtcatc gtgcggccgt cctacctgcg cctcggcttc aacgccgagc
     5941 tcgccgtccg cagccaggag atgatccgcc ccgggggtga ggcggttccc aagctgcggg
     6001 ccacgccgcc ttcgacgaaa gctccgcgcg ccagccgatt tcctaaggga ggtccttgac
     6061 caggacgcgc ccgcacgtgg aaagccagcg cagctgggct cgcgtcagtt cgatggcacc
     6121 tcgggtcgcg agtgcgctca ggcgggagtt ctctcgctca tactcggcca gatccattcg
     6181 catcagcgcg agcgtccgga gcagcccggc ccgggcttcc gcgagaaacc gtttccgggt
     6241 ctccagcggg agcgccgcga ggaagtagat ccggattcgg acgggatcgt gcagcgctcc
     6301 gagatcccca tcgttcaagc cgcgaagcca tccttgtagc gcgcgcctgc cgagggacgt
     6361 cacctcatac agactctttc gctgccgacc gttggcgtcg cgacgagaat gaagaagccc
     6421 gccagcattc aggcgggcga ccaacgggta aatcgcccct gcgctgccgc tgaatcgcac
     6481 gcttcgtgac ct
//
LOCUS       DASSLZ010000195         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_453776, whole genome shotgun sequence.
ACCESSION   DASSLZ010000195 DASSLZ010000000
VERSION     DASSLZ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453776"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFM00_07590"
     CDS             <1..237
                     /locus_tag="VFM00_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498148.1"
                     /translation="NALWAVAERALAALEPQSFGYRRAAPRVALTPSAARAALDAVYA
                     ADRALKRGEVRDAELRDLIERRLMEGEGATGRHG"
     gene            230..2803
                     /gene="leuS"
                     /locus_tag="VFM00_07595"
     CDS             230..2803
                     /gene="leuS"
                     /locus_tag="VFM00_07595"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET7498149.1"
                     /translation="MAEPRPTGPDTAHPAEESRGEERGRSYPFRELEAAARKRWVEER
                     LFELRDPMKAERRFYCLNMFPYPSGDLHVGHGRNYILGDAVARVRKMQRFTVLQPMGW
                     DAFGLPAENAAIKNKTHPAVWTRKNIARMKAQLFAWGVMFDWNREIASCDPSYYRWTQ
                     WIFLKLYEQGLAYRAMGSVNWCPSCQTVLANEQVVDGKCERCGTVVETRELEQWYFKI
                     TDYAEPLLRDLALLEHWPEKVRVMQSNWIGRSEGASVRFPIAGREALLEIFTTRPDTL
                     MGATFMVVAAEHPLVVTLASEGKLSPDAIRFVDRLKRARVENRFAVETEKEGVALGVE
                     AVHPLTGARLPVWVANYVLMGYGTGAIMAVPAHDQRDFEFATRYHLPIVEVIRPAEGP
                     AFDGTAAYEGDGALVHSAPFDGLPAEEGKRAIVSALEKAGKGEARVNWRLRDWLISRQ
                     RYWGAPIPMVTCPSCGMQPVPESELPVLLPEQVEFEPTGKSPLLSNKAFLEAKCPQCG
                     GPARRETDTMDTFVDSSWYFLRFLSPKAEDRAFDTDAVNRWLPVNQYIGGVEHAILHL
                     LYSRFIVKVFQRMGLVSFAEPFEHLFTQGMITKDGAKMSKSHGNVVPPDALIERYGAD
                     TVRLYTLFIGPPEKEAEWNDRGVEGAYRFLTRYWKMIEDFTEANEVVPGSKRPQTAGG
                     GAGGTAYAASGPARDLRRRVHQLVHQITGDVERFHFNTAVSGLMQVVNAVQEYQAQEG
                     DLRAPEAAEAVDLATRLLAPLAPHTAEGAWDRLGHPESVVLAPWPSADPEAQQSASQT
                     LAVQVNGKLRAQIQVPAAASKAEIEQAAQADPKVQKFTEGKQVVQVIVVPGKLVNVVV
                     R"
BASE COUNT          557 a         1080 c         1073 g          459 t
ORIGIN      
        1 aacgcgctct gggccgtggc cgaacgcgcg ctggcggcgc tcgagccgca gtcgttcggc
       61 taccgccgcg ccgcgccgcg cgtcgcgctc acgccgtcgg cggcccgggc ggcgctcgac
      121 gccgtctacg cagccgaccg cgcgctcaag cggggcgagg tgcgggacgc ggagctgcgc
      181 gatctcatcg aacggagact catggaaggg gaaggagcga cgggaagaca tggctgagcc
      241 gcgaccgacc gggccggaca ccgcccaccc ggctgaggaa tcgcgcgggg aggagcgcgg
      301 acggagctat ccgttccgcg agctggaggc cgccgcccgg aagcgctggg tggaggagcg
      361 cctcttcgag ctgcgcgatc cgatgaaggc ggagcgccgc ttctactgcc tcaacatgtt
      421 cccgtatccc tcgggcgatc tccacgtcgg ccacggacgc aactacatcc tcggcgacgc
      481 cgtggcgcgc gtgcggaaga tgcagcgctt cacggtgctc cagccgatgg gctgggacgc
      541 cttcgggctg cccgccgaga acgcggcgat caagaacaag acgcaccccg cggtatggac
      601 gcggaagaac atcgcgcgga tgaaggcgca gctcttcgcc tggggcgtga tgttcgactg
      661 gaaccgcgag atcgcctcct gcgatccgtc ctactaccgg tggacgcagt ggatcttcct
      721 caagctctac gagcaggggc tggcctaccg cgccatgggc tccgtgaact ggtgcccgtc
      781 gtgtcagacc gtgctcgcca acgagcaggt cgtggacggc aagtgcgagc ggtgcggcac
      841 ggtcgtcgag acccgcgagc tggagcagtg gtacttcaag atcaccgact atgccgagcc
      901 gctgctgcgc gacctggcgc tgctcgagca ctggcccgag aaggttcgcg tgatgcaatc
      961 gaactggatc ggccggagcg agggcgcgtc cgttcgcttt ccgatcgcgg ggcgcgaggc
     1021 gctgctcgag atcttcacga cgcgccccga cacgctgatg ggcgcgacgt tcatggtcgt
     1081 ggccgccgag cacccgctcg tcgtgacgct ggcgtcagag ggaaagctct cgccggatgc
     1141 gatccgcttc gtggaccgtc tgaagcgcgc gcgcgtcgag aaccgcttcg ccgtggagac
     1201 cgagaaggag ggggtcgcgc tgggggtgga agccgtgcac ccgctgacgg gcgcgcgcct
     1261 gccggtctgg gtggccaact acgtcctgat gggctacggc acgggcgcga tcatggccgt
     1321 gcccgcgcac gatcagcgcg acttcgaatt cgcgacgcgg taccacctgc cgatcgtcga
     1381 ggtgatccgt cccgccgagg gcccggcatt cgacggaacg gcggcatacg aaggggacgg
     1441 cgctctggtc cactccgcgc cgttcgacgg gctccccgcc gaagagggaa agcgcgcgat
     1501 cgtgagcgcg ctggagaagg cagggaaggg cgaggcgcgc gtgaactggc gcctgcggga
     1561 ctggctgatc tcccggcagc gctactgggg agcgccgatc ccgatggtga cctgcccctc
     1621 gtgcggcatg cagcccgtgc cggagtccga gctgccggtg ctcctgccgg agcaggtcga
     1681 gttcgagccg accgggaagt cgccgctcct ttccaacaag gcgttcctcg aggccaagtg
     1741 tccccagtgc ggcggccccg cccggcgcga gaccgacacc atggacacct tcgtggactc
     1801 gtcgtggtac ttcctgcgtt ttctgagccc aaaggcggaa gaccgtgcgt tcgatacgga
     1861 tgccgtgaac cgctggctcc ccgtcaacca gtacatcggc ggcgtggagc acgccatcct
     1921 gcatcttctc tactcgcgct tcatcgtgaa ggtcttccag cggatggggc tggtctcctt
     1981 cgccgagccc ttcgaacacc tcttcaccca ggggatgatc accaaggacg gcgcgaagat
     2041 gtccaagtcg cacggcaacg tcgtgcctcc ggatgcgctc atcgagcgct acggcgccga
     2101 caccgtccgc ctttacacgc tcttcatcgg cccgcccgaa aaagaggccg aatggaacga
     2161 ccgcggcgtg gaaggtgcct accgattcct cacgcggtac tggaagatga tcgaggattt
     2221 cacggaagcg aacgaggttg tcccgggcag caagcgaccc cagacggcgg gcggcggagc
     2281 cggcgggacc gcttatgccg cgtccggacc ggcccgggat ctgcgccgca gggtccacca
     2341 gctcgtccac cagatcaccg gcgacgtgga gcgcttccac ttcaacaccg ccgtgagcgg
     2401 cctcatgcag gtcgtgaacg cggtccagga ataccaagcc caggaaggag atctgagggc
     2461 ccccgaagcc gccgaagcgg tcgatctggc caccaggctc ctggcccccc tggccccaca
     2521 taccgcggaa ggcgcctggg accgcctggg acaccccgaa tcggtcgtcc tggccccatg
     2581 gccctcggcc gaccccgaag cccagcagag cgcctcccag acgctcgccg tccaggtcaa
     2641 cggcaagctg agggcccaga tccaggtccc ggcagccgcc tccaaggccg aaatcgagca
     2701 agccgcccag gccgacccca aggtccagaa gttcaccgag ggcaagcagg tcgtccaggt
     2761 catcgtagtc ccaggcaagc tggtcaacgt agtcgtccgg tagcggcctg ggagtcaatg
     2821 gcggccgaac gcggaagacc gaaggccgac cgcttcgacc aagcaccaac cccgtctgcg
     2881 atcgtcagag gggaaccggg gagtaggggc gccaacggta ttcatgtcaa aacttcacgc
     2941 ccaagacccg gcaggccgag ccgatctggg ccctggcgtg aagttgacat aatacccatt
     3001 atgcgacatg cgcccttcga atggctgccc agacccaacc tcgcttggga tcccaggcca
     3061 ttcaacgtga agccgccccc aagactccaa ctcgcttggg atccccggca catgacgtga
     3121 aagccgttcg atcgcgcctt cgggaacggc ggccgccgcg cctccggac
//
LOCUS       DASSLZ010000196         5752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1466453, whole genome shotgun sequence.
ACCESSION   DASSLZ010000196 DASSLZ010000000
VERSION     DASSLZ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466453"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VFM00_07600"
     CDS             <1..786
                     /locus_tag="VFM00_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7498150.1"
                     /translation="RELLEAARESATIPVVSRLTMSEEELVAADGEPLPVPWPAGADE
                     MPPAPPAIEPPTARAPDEPEGASAPQRTEAADAAARAGILKELNALIAGLGELATGLD
                     APASSSGDGGGRTPAQLVDAVVHTAADGIEDLAAGLMRANELAGVAERVTNKATLLAL
                     NAALEATRSGSEAFAAIAEETRRLAEFAREATDTIARLSSEIEYKVGETITAIHATSE
                     DAKSVVATFQASPGSSSPIPTAAVEALLRRAQELKRRLLAEAA"
     gene            783..2432
                     /locus_tag="VFM00_07605"
     CDS             783..2432
                     /locus_tag="VFM00_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498151.1"
                     /translation="MRTSASEHLSQDQVMARLAATFSALVKEQIDAVERYLEHDDPDH
                     DSSDIAGLVELAVRLVERSAEWLGVSEMASLALELREALLQLGQLRPEHREEMVAHCR
                     VALAAEARLAEKLKTEGFGALVHHAGIVGDAVEQLRAGLSSAKSEALSASRGVIDETV
                     PSVGPSENLLALTFEIKNALAHQNDRIASMSETVGETLRSAQTVSAEWEAIVKKVERR
                     RMQAGAKAGDDPLDGKALVVHQELQETASGLRTLSQELGQLLSLQYALERRARDLDEN
                     LLWEFLDPLDRFVDDLYAAVTRRDGETRRTVLTLQTGGVGFEPEIGSLLLPLLIRLLE
                     SAKPCDEGCEEEEIRLTAAREGLEARIALEGTCCFDEEAERALMEALAGLGGFATIER
                     LMPAGTSIHLQFPMARSLRSFLIVEAAGQRIALPWSAVERIHASPEDMGWDGPANGPV
                     HALASLFSSGGDPAGAADAPPGTAVPGRPVAVIRSGSASAVIGFDRIVWRENARLTPL
                     PPRLYPVDEVLGGIVAPDNSVTLVLNPAGVIRRLVGGEASA"
     gene            2429..4093
                     /locus_tag="VFM00_07610"
     CDS             2429..4093
                     /locus_tag="VFM00_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7498152.1"
                     /translation="MKSLRIERLGSRDEFRFALQGEVDDAGARSLDRLLFECQARGAA
                     KVELDFGGVSRLCTLATSVLVRQGKVYRETERCITVRGLRGEARAAVDEAGAVLFEVN
                     GNDAPATAVACADAVTPAPSLPSSVVSASSAVDLPVLQTKLRRKIIEFRNLFEITRAL
                     NLALDLEEVLNLLGLSVMGQFGVERLAIFLSDADRGGVLVPRQVRGFAQGHFKDLRIP
                     PEAVERGAGVETFFLVDARPGEASPLAELKRSGLEWGLALRVRSELIGVLLIGGRSGR
                     SGFQEDERDLLTTLAHQGAVAIANARFHRVQEERNLGLVRGMMALLESRDPFARGGTE
                     RVVRYVTAMARVLHYPREQVKSLIYGAVLRDIGMITVSGLILKNPAHLSEEEWALIKQ
                     HPVRGAQILEEMTFPKEVLDVVRNHHERWGGEGYPAGIRGQEIPLGARIVSLVDAYVA
                     MTMERPYRRALPYEKARQVIAENWGVPFDPELVLVFLSVLDKVESRSRLNRAGGAPPP
                     NGTEEEAPPLKAELDPLASGAVIPTSHAAVNDDPEPSDPVSRETSS"
     gene            4090..5730
                     /locus_tag="VFM00_07615"
     CDS             4090..5730
                     /locus_tag="VFM00_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7498153.1"
                     /translation="MSIDSLRPVSDPASQKAKRTILVVDDDRRVIDLLQISLTQNGYK
                     VNTALTGEEGLESVRREAPDLIILDLRLPKKTGFEVCAALKSSKDTAHIPIVMVSASA
                     EVDARLQGLMHGADDYLTKPFSPKELLIKVRRIFERIEKTELLSTKNKELETEVARNR
                     EDLLVRNKELRFQVYSLETLMELTHQLNSSLDLDHTLNTLILSVVGQLRVNSACLFLT
                     DDREDPTRLDPCTFKGLKEEQARGISFEYSGPFVRALAPLDGDEIQPHRLADLEHDTD
                     LDGEVGALFAAGLTLVCPVVMKQRLTAILAVGEKVSGQEFQAHDLEMLRVLSESAGMA
                     IENARLFKDLQEAYVSTVRLLVSRIEEKDPYTHGHTERVAAYAVGIAKEMGFVPEEVQ
                     RIQFGAFLHDIGKVHTEDHVLHKPGALTEEEWRIVKMHPLRGAEMVKGVRFLERVTDM
                     IRHHHERVDGRGYPDGLKGDQISTAAKIVNVADAFDAMTTDRPYRAGLTMEQAIGQMT
                     EKAGTQFAAEIVEVFVRGLESGSIKLIQNSSPYVAPQR"
BASE COUNT          913 a         1974 c         2051 g          814 t
ORIGIN      
        1 cgcgagctgc tcgaggcggc gcgcgagagc gcgacgatcc ccgtggtgag ccgcctcacg
       61 atgagcgagg aggagctggt cgccgccgac ggcgagccgc tccccgttcc gtggccggcc
      121 ggagcggacg aaatgccgcc ggccccgccc gcgatcgagc cgcctacggc gcgcgcgccc
      181 gatgaacccg agggggcttc cgcgccgcag cgcaccgaag ccgccgacgc ggccgcgcgc
      241 gcgggaatcc tgaaggagct gaacgcgctg atcgccgggc tgggcgagct ggccaccggg
      301 cttgacgccc cggcgtcgtc gtcgggcgac ggcgggggcc gcacgccggc gcagctggtg
      361 gacgcggtcg tgcacaccgc cgccgacggc atcgaggatc tcgccgcggg gctcatgcgc
      421 gcgaacgagc tcgcgggcgt ggcggagcgc gtcacgaaca aggccaccct gctcgccctg
      481 aacgccgcgc tcgaggccac gcgttccggg agcgaggcct tcgcggccat cgccgaggag
      541 acccgccgcc tggccgaatt cgcgcgcgag gcgaccgaca ccatcgcccg cctctcgagc
      601 gagatcgagt acaaggtggg cgagaccatc accgcgatcc acgcgaccag cgaggatgcc
      661 aagtcggtgg tggccacgtt ccaggcttcc ccggggagct cgtcgcccat cccgaccgcc
      721 gcggtcgagg cgctccttcg ccgcgcccag gagctgaagc gccgcctcct ggccgaagcc
      781 gcatgaggac ctccgcctcc gaacacctga gccaggacca ggtgatggcc cggctcgcgg
      841 ccaccttcag cgccctcgtg aaggagcaga tcgacgccgt cgagcgatac ctcgaacacg
      901 acgatcccga tcatgattcc agcgacatcg ccgggctcgt ggagctggcg gtgcggctcg
      961 tggagcggag cgccgaatgg ctgggcgtga gcgagatggc gagcctcgcg ctggagcttc
     1021 gggaggcgct gctccagctg gggcagctgc gccccgagca ccgcgaggag atggtcgcgc
     1081 actgccgcgt ggcgctggcc gccgaggcgc ggctggcgga gaagctgaag acggagggat
     1141 tcggcgcgct ggtccaccac gccggcatcg tcggcgacgc ggtcgagcag cttcgcgccg
     1201 ggctctcctc ggccaagtcg gaggccctct ccgcctcgcg cggcgtgatc gacgagacgg
     1261 tgcccagcgt ggggccgagc gagaacctgc tcgcgctcac gttcgagatc aagaacgcgc
     1321 tggcgcacca gaacgaccgc atcgcttcga tgagcgagac ggtgggcgag acgctccgct
     1381 cggcgcagac ggtgtcggcc gagtgggagg cgatcgtgaa gaaggtggag cggcggcgca
     1441 tgcaggcggg agccaaggcg ggcgacgacc cgctcgacgg gaaagcgctc gtcgtgcatc
     1501 aggagctcca ggagacggcg tcgggccttc ggaccctctc gcaggagctg ggacagctcc
     1561 tgagcctcca gtacgcactg gagcggcgcg cccgcgatct ggacgagaac cttctctggg
     1621 agttcctcga cccactcgac cgcttcgtgg acgatctcta cgcggccgtc acccggcgcg
     1681 acggcgagac ccgccgtacc gtgctcaccc tgcagaccgg cggcgtcggc ttcgagccgg
     1741 agatcgggtc gctgcttctt ccgctcctga tccgcctgct cgagagcgcc aagccgtgcg
     1801 acgaagggtg cgaggaagag gagatccggc tcaccgccgc ccgcgagggg ctggaggcgc
     1861 ggatcgccct cgagggaacc tgctgcttcg acgaagaggc cgagcgcgcg ctcatggagg
     1921 cgctcgcggg tctcggcggc ttcgcgacga tcgagcggct catgcccgcc ggcacgtcga
     1981 ttcatctcca gttcccgatg gcgcgatcgc tgcgctcctt cctgatcgtc gaggcggccg
     2041 gacagcggat cgcccttccc tggagcgcgg tcgagcggat ccacgcctcc cccgaggaca
     2101 tgggatggga cggaccggcg aacggccccg tacacgcgct cgcgtcactc ttcagctccg
     2161 gcggggaccc cgcgggggcg gccgacgccc cgcccggcac ggccgtcccg ggccgccccg
     2221 tcgcggtgat ccgctcgggg agcgcctcgg cggtgatcgg cttcgaccgg atcgtctggc
     2281 gtgagaacgc gcgcctgacg ccgcttccgc cgcggctcta tccggtcgac gaagtgctcg
     2341 gcggcatcgt cgcgccggac aacagcgtca ccctcgtgct gaacccggcc ggagtcatcc
     2401 ggcggcttgt cggcggcgag gcgtcggcgt gaaatcgctc cgcatcgagc gcctcgggtc
     2461 gcgcgacgag ttccggttcg cattgcaggg agaggtggac gacgccgggg cccgttcgct
     2521 cgaccggctc ctcttcgagt gccaggcccg gggcgccgcc aaggtcgagc tggacttcgg
     2581 cggcgtgagc cgcctctgca cgctcgcgac gtcggtgctc gtccgccagg ggaaggtcta
     2641 ccgggaaacg gagcgctgca tcacggtccg gggtctgcgc ggcgaggccc gcgcggcggt
     2701 ggacgaagcg ggcgccgttc tgttcgaggt caatggcaac gacgctccgg ccacggcggt
     2761 cgcctgcgcc gatgcggtca cgcccgcgcc gagcctcccg tcgtccgtgg tctccgcctc
     2821 gagcgcggtg gatctcccgg tcctccagac gaagctccgc cgcaagatca tcgagttccg
     2881 gaacctcttc gagatcacgc gcgcgctgaa tctcgcgctc gatctcgaag aggtactgaa
     2941 cctgctcggg ctgagcgtca tggggcagtt cggcgtggag cggctcgcga tcttcctgag
     3001 cgacgccgat cgcggcgggg tcctcgtccc gcgccaggtg cgcggcttcg cgcaggggca
     3061 tttcaaggat ctccggattc cacccgaggc cgtggagcgc ggcgccggcg tcgagacgtt
     3121 cttcctcgtg gacgcacgcc cgggcgaagc gtcgccgctc gccgagctga agcgctccgg
     3181 gttggagtgg gggctggcgc ttcgcgtccg ctcggagctg atcggcgtgc tcctgatcgg
     3241 tggccggagc ggccggagcg gcttccagga ggacgagcgg gatcttctca ccaccctcgc
     3301 gcaccagggc gccgtggcga tcgccaacgc acgcttccac cgcgtccagg aggagcggaa
     3361 cctgggattg gtgcggggga tgatggcgct cctcgagagc cgcgatccgt tcgcgcgagg
     3421 cggcaccgag cgggtcgtgc gctacgtcac cgccatggcc cgcgtgctcc actatccgcg
     3481 ggagcaggtg aagtcgctca tctacggcgc cgtgctgcgc gacatcggca tgatcaccgt
     3541 gagcgggctg atcctcaaga atccggcgca tctctccgag gaggagtggg cgctcatcaa
     3601 gcagcatccc gtgcgcggcg ctcagatcct cgaggagatg accttcccga aggaagtgct
     3661 cgacgtggtt cgaaaccatc acgagcgatg gggcggcgag gggtacccgg ccgggatccg
     3721 gggccaggag atcccgctcg gcgcgcgcat cgtctccttg gtcgacgcct acgtggcgat
     3781 gaccatggag cgcccctacc ggcgcgcgct cccctacgag aaggcgcggc aggtgatcgc
     3841 ggagaactgg ggcgtgccgt tcgatccgga gctggtcctg gtcttcctga gcgtcctcga
     3901 caaggtggag tcccgctcgc ggctgaaccg ggcgggaggg gcgccgccgc ccaacggaac
     3961 ggaagaagaa gcccccccgc tgaaggcgga gctggatccg ctcgcttccg gagcggtcat
     4021 tcccacgagc cacgccgccg tgaacgacga ccccgagccg tccgaccccg tttccaggga
     4081 gacctcctcg tgagcatcga cagcctccgt cccgtgtccg atccggcctc gcagaaggcc
     4141 aagcggacca tcctggtcgt cgacgacgac cggcgcgtga tcgacctgct ccagatctcg
     4201 ctcacgcaga acggctacaa ggtgaacacc gccttgaccg gagaagaggg gctggagtcg
     4261 gtgcgccggg aagcgcccga tctgatcatc ctggacctgc gcctccccaa gaagaccggc
     4321 ttcgaggtct gcgccgcgct gaagagcagc aaggacacgg cgcacatccc gatcgtcatg
     4381 gtctccgcga gcgccgaggt ggacgcgcgc ctccagggcc tgatgcatgg ggccgacgac
     4441 tacctgacca agcccttctc gccgaaggag ctgttgatca aggtgcggcg catcttcgag
     4501 cggatcgaga aaaccgagct cctctccacg aagaacaagg agctggagac cgaggtggcg
     4561 cgcaatcgcg aggatctcct ggtccgcaac aaggagctcc gtttccaggt ctacagtctc
     4621 gagacgctga tggagctgac gcaccagctc aactcctccc tcgacctgga ccacacgctg
     4681 aacacgctga tcctgagcgt cgtggggcag ctccgggtca attcggcgtg cctcttcctc
     4741 accgacgacc gcgaggaccc gacccgcctg gacccctgca cgttcaaggg gctgaaagag
     4801 gagcaggcgc gcggcatctc cttcgagtac agcggcccgt tcgtccgcgc gctggcaccg
     4861 ctggacggcg acgagatcca gccgcaccgc ctggccgatc tggagcacga caccgatctg
     4921 gacggggagg tgggggcgct gttcgccgcc gggctcacgc tggtctgccc ggtcgtcatg
     4981 aagcagcggc tgacggcgat cctcgcggtg ggggagaagg tgagcgggca ggaattccag
     5041 gcccacgacc tcgagatgct gcgcgttctc tcggaatcgg ccggcatggc gatcgagaac
     5101 gcgcgcctct tcaaggatct tcaggaggcg tacgtctcca ccgtccgtct cctcgtgtcc
     5161 cggatcgaag agaaggatcc ctacacgcac ggccatacgg agcgggtggc ggcctacgcc
     5221 gtggggatcg cgaaggagat gggcttcgtg ccggaggagg tgcagcggat ccagttcggc
     5281 gccttcctgc acgacatcgg aaaggtccac accgaggacc acgtgctcca caagccgggg
     5341 gccctgaccg aagaagagtg gcgcatcgtg aagatgcatc ctctgcgcgg cgccgagatg
     5401 gtcaaggggg tccgtttcct cgagcgcgtg accgacatga tccgccacca ccacgagcgc
     5461 gtggacggcc gggggtatcc ggacgggctg aagggagacc agatctcgac cgcggccaag
     5521 atcgtgaacg tggccgacgc cttcgacgcc atgaccacgg accgtcccta ccgcgcgggc
     5581 ctcaccatgg agcaggcgat cggccagatg accgagaagg cgggcacgca gttcgcggcg
     5641 gagatcgtcg aggtcttcgt gaggggattg gagtcgggga gcatcaagct gatccagaac
     5701 agctcgccgt acgtcgctcc gcagcgctga gccggctcag cgcgccgcgc tg
//
LOCUS       DASSLZ010000197         7990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1071416, whole genome shotgun sequence.
ACCESSION   DASSLZ010000197 DASSLZ010000000
VERSION     DASSLZ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071416"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2784)
                     /locus_tag="VFM00_07620"
     CDS             complement(<1..2784)
                     /locus_tag="VFM00_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7498154.1"
                     /translation="MTRSTAAGEGLLRGSLLFLLLVLALAPCPRSAAAEKASTGVLAG
                     VVLDQEDRQPLPNARVALYRMSPGDSVWTMVKEGLTGADGSFRYEVPAGTYRLILYAQ
                     SYTVNVRDDLVITPGATVEISATLTPRPLEIQGVEVQGEQKHDTETSALTKRKKSEAV
                     TDGISAEQIAKSTDANAAEALQRVTGLSLVGGRYVYVRGMGERYSSTQVNGASLGTPE
                     PNKRVVPLDLFPAGTLDEIVVQKTYSPDQEGEFAGGVIQLHTRDAVEGRRLAQSLSTG
                     YSSPTLGGKRLTYSGGSLDFLGYDDGTRDLPDAFRRVAGSGRLVPGGVFGGGLTPAQI
                     QAAGRSFNKTWSPERDDSRPNYGYSGTYSQALRVLGKPLSFVGSLSLSNNIATRDRDN
                     NAYAGTSTRLTPLYLYKVEETTRNTLGGALANWNLRLSSGHSIGLRALYTRSSEDNAR
                     IMQGPNFNFGTDLVRISSLNYIERGLFSGVLSGEHTVGPLGGLRLDWRTGYSEAMRDE
                     PDRRESVYESNGVGGVYLSHRVQDPLTRVFGDMNEYDRSAAADLTKSFKLSGARALRL
                     KTGGFLRHRNRISGFRRLAFVMGAAARDSLDTTLPPEELLADENIKPRWFSLIEETRE
                     NDQYRARQHILAGYGMAELEVVPGVTLQGGVRNERSDQVVEAKSPFVTNATETDARLI
                     DNDFLPAANATWRLTERMNARLGYSVTVSRPELREMSPFDMYDYETGYSEVGNPEIRS
                     TIIQNYDARWELYPGPRELVSVSGFRKVLFQPIESIVLGSSGGYVLMPRNGRDGRVRG
                     MELEARVGLSRLWDAAARVLPFGGHGAGLDHWALSMNYARVESSVRVQTRLDGNGAPF
                     YREGPLQGQSTYSLNAGLYYGSNAMDGSLLLSAFGKRLAQVGAGATQSSLPDIYEHPP
                     LSLDMTVSRSLFGS"
     gene            complement(2814..4559)
                     /locus_tag="VFM00_07625"
     CDS             complement(2814..4559)
                     /locus_tag="VFM00_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7498155.1"
                     /translation="MTKRMFGILALAGSLIVAAAAPTVHGASYPERLVFDGNVLWNNM
                     QAFPAQLPWAGSGPCTTSVATTLTTADLGTVYFTHNKTNADPKLVDPFNLTSPRWDPQ
                     QMSPLNCKYSSDAVVMNVTSLNPWFEQTDYVGAVPYRLADPSRDWTTGWIYTNTAGGM
                     GRTDINYAKPLVILQGAYATMTLSASNNYLLRGKVEIAPGFQWTIPAGTYLFGEKATT
                     GYLVVDRGAKIFVNGTAAAPVVLTSDQDPTLGLMAPSDNGGLVIHGRAVANCANTVAG
                     DSCVSEGGAGAYGGNNDHDNSGSIKYMRIEYSGKTISPDNELNSLTMNAVGDGTVIDY
                     VNACLGSDDAFEWFGGNGRAKHLVAWGEDDDGLDWQLGWRGMVQFAVVQQHPGRGDKG
                     IEADNSEFDFQAQPRSNPTLANLTLVGTNPPTAGAGTTNIGVHWRRGTAGTIVNSVVL
                     GFRGPGLQLSDPETFANCPGTGPAVYCTPAVSAVETSEATLPKGIFMTASPNPLRSST
                     DILFGLPADQRNVRVQIFDARGRLVETLATGPMARGVHHVSWAPRGSMPAGQYFFRVA
                     GDQGGTTSGKLVLVR"
     gene            complement(4738..6933)
                     /locus_tag="VFM00_07630"
     CDS             complement(4738..6933)
                     /locus_tag="VFM00_07630"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HET7498156.1"
                     /translation="MSHRIRTATLILLGVALFAPRGVWAARAELAGEAAARAVAVSAP
                     TLDAPASVSTATDQPLSIQAVASDADAGDVLTITAMGYPAGLSLAGVPSVTPATATLS
                     GTLGLADAGSYSIEWSVSDGTGGTAAAATALTVVENQPPVLNAPATASGAETVPLSFS
                     VTVSDPDGDTVTSLNASGLPSGATFTPNALKTIGLFEWTPPLGSAGAHTVGFTASSGS
                     PVRTSSVSTTVTVGPKDNPPVITSPSTVNAVATYPTSFVVSVSDPDGNPITKLEIEGT
                     QNTALPAGAIVTINASNTSATFSWTPTAAQVGNYGIDEIAASGFLQVVKVTRVVVRDD
                     RAPVVTAPASIVGAENVAVSFAVSASDPDGHPIASLTAAPLPLGASFTANAANTSGTF
                     QWTPGFSQAGSYPVTFTASNALAGSATATLSISDQNRAPVANAGGPYSGIVGLPVSFD
                     GSASSDPDGNVLTYAWDYGDGATGTGAMPSHAYLAGGAYTVSLTVTDNGTPALSTTAS
                     ATATIAASFDARVYTTNANRSIRLNSGKPRWCAQVEPIGGDFSIADVNVATIVLKYGA
                     SQIAVEAGKGASTGDLDGNGVDELTVCFSKAALRTLFAGLPIGQNHVTLTVEASLMSG
                     ARVVGAIEVDVFGSGASLAATVTPNPLNPEAVLSFATRSVGALRVLLYDVNGRLVRVL
                     LDETTASAGYHDVRVDGHGAHGEPLASGTYFFRIVSADGVSAGRLTILK"
     gene            complement(7138..7857)
                     /locus_tag="VFM00_07635"
     CDS             complement(7138..7857)
                     /locus_tag="VFM00_07635"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET7498157.1"
                     /translation="MTCALSVNVDHVATLREARGGTEPDPARAAALALDGGAAGITVH
                     LREDRRHIQDDDVRRLRAMRRGELNLEMALTEEMLAIALDLRPERSTLVPERREELTT
                     EGGLDLMRHAPAVRRAAPRLREREITLSLFLDPEPALAPVARDCGAAMVEIHTGAYAN
                     ARGEAALAAELDRIALAARAFHAVGLEVHAGHGITTANVGPLLRAFPFTELSIGHHIV
                     SLAIEVGMRAAVEAMLAAMKI"
BASE COUNT         1182 a         2669 c         2856 g         1283 t
ORIGIN      
        1 cgatccgaag aggcttcggc tcaccgtcat gtccaacgag agcggggggt gctcgtagat
       61 gtcgggcaag ctggactgcg tcgcccccgc tcccacctgc gccaaccgct tcccgaacgc
      121 gctcaggagc agcgagccgt ccatcgcgtt cgatccgtaa tagagccccg cgttgagcga
      181 gtaggttgac tgcccctgga gcgggccctc ccggtagaag ggtgccccgt tcccgtcgag
      241 ccgcgtctgc acgcggacgc tcgactccac ccgcgcgtag ttcatgctga gcgcccagtg
      301 atcgaggccg gcgccgtgcc cgccgaacgg gagcacccgg gcggccgcgt cccagaggcg
      361 ggagaggccg acccgagcct ccagctccat gccgcggacg cggccgtcac ggccgttgcg
      421 gggcatcagg acgtagcctc ctgaggagcc gagcacgatg ctctcgatcg gctgaaagag
      481 caccttgcgg aagccgctca ccgagaccag ctcccgcggc ccgggataga gctcccaccg
      541 ggcgtcgtag ttctggatga tggtggaccg gatctcggga ttgccgacct ccgagtagcc
      601 ggtctcgtag tcgtacatgt cgaagggact catctcccga agctcgggac gggacaccgt
      661 gacgctgtag ccgagccgcg cgttcatccg ctcggtgagg cgccacgtgg cgttcgcggc
      721 cgggaggaag tcgttatcga tgagccgcgc gtcggtctcc gtggcgttcg tcacgaaggg
      781 actcttcgcc tcgaccacct ggtcggatcg ctcgttccgc acaccaccct gcagggtcac
      841 gccgggcacc acctcgagct cggccatccc atagcccgcc aggatgtgct gcctggcgcg
      901 gtactgatcg ttctcgcgcg tctcctcgat cagcgagaac cagcgcggct tgatgttctc
      961 atcggcgagg agctcctcgg ggggcagggt cgtgtccagc gagtcgcgcg cggccgcgcc
     1021 catcacgaac gcgagccggc ggaaaccgga gatgcggttt cggtggcgca ggaatccccc
     1081 ggtcttgagg cgcagggcgc gcgcgccgga gagcttgaag ctcttcgtga ggtcggcggc
     1141 ggcggagcgg tcgtactcgt tcatgtcgcc gaacacccgg gtgagcgggt cctggacccg
     1201 atgggagaga tagacgccgc ccaccccgtt cgattcgtag acgctctcgc ggcgatcggg
     1261 ctcgtcgcgc atcgcctcgg agtagccggt gcgccagtcg agccgaaggc cgcccagcgg
     1321 gccaacggtg tgctcgccgc tcaggacgcc ggagaagagt ccacgctcga tgtagttcag
     1381 gctggagatc cgcacgagat cggttccgaa attgaaattg gggccctgca tgatccgggc
     1441 gttgtcctcg gagctgcgcg tgtagagtgc gcgcaacccg atggaatgcc cgcttgagag
     1501 ccgcaggttc cagttcgcga gcgcgccgcc cagcgtgttc cgcgtggtct cttccacctt
     1561 gtagaggtag aggggggtga gccgcgtgga cgtgccggcg taggcgttgt tgtcgcgatc
     1621 ccgggtcgcg atgttgttcg acaacgagag cgacccgacg aacgagagcg gctttcccaa
     1681 gacgcgaagc gcctggctgt aggtgccgga atagccgtaa ttgggccgcg aatcgtcccg
     1741 ctccgggctc cacgtcttgt tgaacgaacg gcccgccgcc tggatctgcg ccggggtcag
     1801 cccgccgccg aagaccccgc cgggtaccag ccggccgctt ccggcgactc tccggaacgc
     1861 gtcgggaagg tctcgcgtgc cgtcgtcgta gcccaggaag tcgagcgaac cgccggagta
     1921 ggtgagacgc tttccgccga gggtcgggct ggagtaaccc gtcgagaggc tctgcgccag
     1981 gcgccttccc tccacggcat cccgcgtgtg cagctggatc accccgccgg cgaactcccc
     2041 ttcctggtcg ggcgaatagg tcttctggac gacgatctcg tccagcgtcc ccgccggaaa
     2101 gaggtcgagc gggacgaccc gcttgttcgg ctcgggcgtg ccgaggctgg ctccgttcac
     2161 ctgggtcgag ctgtaccgct cgcccatccc gcgtacgtag acgtatcgcc cccccaccag
     2221 cgacagcccc gtcacgcgct ggagcgcctc ggccgcgttg gcgtcggtgc tcttcgcgat
     2281 ctgctcggcg ctgatcccat ccgtaaccgc ctccgacttc ttccgcttcg tcagcgccga
     2341 tgtctcggtg tcgtgcttct gctctccctg gacctcgacg ccctggatct ccagcggccg
     2401 tggcgtgagc gtcgcactga tctcgaccgt tgcgcccggc gtgatgacga ggtcgtcgcg
     2461 aacgttcacg gtgtacgact gcgcgtagag gatcaggcgg tacgtgcccg ccggcacctc
     2521 gtagcggaac gagccgtcgg cgccggtgag cccctccttc accatggtcc agacggaatc
     2581 gcccggactc atccgataga gcgcgacgcg cgcgttcggc agcggctggc ggtcttcctg
     2641 gtcgagaacg actccggcca gaacgccggt ggaagccttc tcagccgcgg cggaacgcgg
     2701 gcacggcgcg agggcgagaa cgaggagaag gaacaggagg gacccccgaa ggagtccctc
     2761 ccctgcggcg gtgcttcggg tcacgtgtcg aatcgggttc agggtcgcgc tccctagcgc
     2821 accagcacca gcttgccgga cgtggtcccg ccctggtcgc cggcgacgcg gaagaagtac
     2881 tgccccgcgg gcatgctccc tctcggggcc cacgagacgt ggtggacgcc gcgggccatg
     2941 gggcccgtgg cgagcgtctc gaccaggcgg ccgcgcgcgt cgaagatctg cacgcgcacg
     3001 ttccgctgat cggccggtag cccgaacagg atgtcggtgc tggagcggag cggattcggg
     3061 cttgccgtca tgaagatgcc cttgggaagg gtggcctcgg aggtctccac cgccgaaacg
     3121 gccggcgtgc agtagaccgc cggtccggtg ccagggcagt tcgcgaacgt ttcagggtcg
     3181 ctgagctgga ggccggggcc gcggaagccc aagaccaccg agttcacgat cgtgcccgcg
     3241 gtgccgcgcc gccagtgcac gccgatgttc gtggtgccgg cgcccgccgt cggcgggttc
     3301 gtgccgacca gcgtgagatt cgccagggtc gggttggagc gcggctgcgc ctggaagtcg
     3361 aactcggagt tgtccgcctc gatccccttg tcgccgcgac cggggtgctg ctgcacgacc
     3421 gcgaactgca ccatgccccg ccatccgagc tgccagtcga ggccatcgtc gtcctcgccc
     3481 cacgcgacca ggtgcttggc gcggccgttg ccgccgaacc actcgaacgc gtcgtcgctg
     3541 cccaggcacg cgttgacgta atcgatcacc gtcccgtcgc cgacggcgtt catcgtgagg
     3601 gagttcagct cgttgtccgg ggagatcgtc ttgccggagt actcgatccg catgtatttg
     3661 atcgagccgc tgttgtcgtg gtcgttgttg ccgccgtagg cgcccgcgcc tccctccgac
     3721 acgcaggagt cgccggccac ggtgttggcg cagttggcga ccgcgcggcc atggatcacc
     3781 agcccgccgt tgtcgctggg agccatgaga cccagcgtcg gatcctggtc gctcgtcaag
     3841 accaccgggg cggccgccgt tccgttcacg aagatcttgg cgccgcggtc cacgaccagg
     3901 tagccggtcg tggccttctc cccgaagaga tacgtgcccg ccgggatcgt ccactggaac
     3961 ccgggagcga tctccacctt gccgcggagg agatagttgt tcgaagcgct cagcgtcatg
     4021 gtggcgtagg cgccctgcag gatgaccagc ggcttcgcgt agttgatgtc ggtccgcccc
     4081 atgccgccgg cggtgttcgt atagatccac ccggtggtcc agtcccggga cgggtcggcg
     4141 agccgatacg gcaccgcgcc cacgtagtcc gtctgctcga accacggatt cagcgaggtc
     4201 acgttcatca cgaccgcgtc gctcgaatac ttgcagttga gagggctcat ctgctgcgga
     4261 tcccagcgcg ggctggtcag gttgaaggga tccaccagct tgggatcggc gttcgtcttg
     4321 ttgtgggtga agtagaccgt gccgagatcg gccgtcgtga gcgtggtcgc cacactggtc
     4381 gtgcagggac ccgatcccgc ccacgggagc tgcgccggga aggcctgcat gttgttccag
     4441 agcacgttgc cgtcgaagac cagtcgctcc gggtagctcg ccccgtgcac cgtcggtgcg
     4501 gcggcggcga cgatgagcga tccggccagc gcgagaatcc cgaacatccg cttcgtcatg
     4561 acgagtctcc tgtgggtcat ccgccgcgtg attccgcttg tcgtggtgtt tcgccgcccg
     4621 gctcgatgac ggggcgaagc taccccggtc cgcgtaacga tgtcgtttcg tcgctcggtc
     4681 ttccgtgtta cgcggtccag catcgctccc gccatcggcc ggacgttgat tcccgcgtta
     4741 cttcaagatc gtgaggcggc cggcggacac cccgtcggcg ctcacgatgc ggaagaagta
     4801 cgttcctgag gccagcggct cgccgtgcgc gccatggccg tcgacccgga cgtcgtggta
     4861 cccggccgag gcggtggttt cgtcgaggag cacgcgcacc agccggccgt tcacgtcgta
     4921 gaggagcacg cggagcgcgc ccacgctccg ggtggcgaag ctcagcaccg cctccggatt
     4981 gagcggattc ggcgtcacgg tcgcggccag ggaagcaccg ctcccgaaca cgtccacctc
     5041 gatggccccg acgaccctgg ctccgctcat gagcgaagcc tcgaccgtga gcgtcacgtg
     5101 gttctgcccg atgggaagac cggcgaacag cgtgcgaagc gcggccttcg agaagcagac
     5161 cgtgagctcg tcgacgccat tgccgtcgag gtcgcccgtg ctcgctccct tgccggcctc
     5221 gacggcgatc tgagacgcgc cgtacttgag gacgatcgtc gccacattca cgtcggcgat
     5281 cgagaagtcg ccgccgatcg gctcgacctg cgcgcaccag cgcggcttgc cggaattcaa
     5341 ccggatcgac ctgttcgcgt tggtcgtgta cacccgcgcg tcgaagctcg cggcgatggt
     5401 cgccgttgcg ctcgcggtag tggagagcgc gggcgtcccg ttgtcggtca ccgtgagcga
     5461 aacggtgtaa gcgccgccgg ccaggtacgc gtggctgggc atggcgcccg tgcccgtcgc
     5521 cccgtcaccg taatcccagg cgtaggtcag gacgtttccg tcgggatcgg acgaggcgga
     5581 tccgtcgaag gagacgggaa gcccgacgat gccgctgtag gggccgccgg cgttcgccac
     5641 cggcgctcga ttctggtccg agatcgaaag ggtcgcggtc gccgatccgg cgagggcatt
     5701 cgaggcggta aacgtcaccg ggtaggagcc ggcctgggag aagccgggcg tccactggaa
     5761 cgtccccgag gtgttggcgg cgttcgcggt gaagctcgcg cccaggggga gcggagccgc
     5821 ggtcagggaa gcgatcggat gcccgtcggg atcggaggcc gagaccgcga agctcacggc
     5881 gacgttctcc gcgccgacga tcgaggccgg agccgtgacc accggagcgc gatcgtcgcg
     5941 gaccaccacg cgcgtgacct tgacgacctg aaggaatccg gatgccgcga tctcgtcgat
     6001 gccgtagttg cccacctgcg ccgccgtcgg cgtccaggag aaggtcgccg acgtattgct
     6061 tgcgttgatc gtcacgatcg ccccggcggg gagcgccgtg ttctgcgtcc cctcgatctc
     6121 cagcttcgtg atcggattgc cgtcgggatc ggacacgctt acgacgaacg aggtcggata
     6181 ggtggcgacc gcgttgaccg tactcggcga ggtgatgacc ggcgggttgt ccttcgggcc
     6241 gaccgtcacc gtcgtggaga ccgaggaggt ccgaaccggc gagccggacg acgcggtgaa
     6301 gccgaccgtg tgggcgcccg cgctgccgag cggcggcgtc cactcgaaga ggccgatcgt
     6361 cttgagcgcg ttcggcgtga aggtcgcgcc ggagggtagg ccggaagcgt tcaggcttgt
     6421 gaccgtgtcg ccgtccggat cggagaccgt gaccgagaag gaaaggggca ccgtctcggc
     6481 gccgctcgcg gtggccgggg cgttcaggac gggaggctgg ttctccacga cggtgagcgc
     6541 ggtcgcggcc gcggccgttc cgccggttcc gtcggacacc gaccactcga tcgagtacga
     6601 acccgcgtcc gcaagtccga gggttcccga gagggtcgcg gtcgccggag tgaccgaggg
     6661 cactcccgcg agggagaggc cggcgggata gcccatcgcc gtgatggtga ggacatcgcc
     6721 ggcgtcggcg tcgctcgcga ccgcctggat gctgagcggc tggtcggtcg cggtcgaaac
     6781 cgaggcgggc gcgtcgagcg tgggcgcgct gaccgcgacg gcgcgggcgg cggcttcgcc
     6841 ggcgagctcc gccctggcgg cccagactcc gcgcggcgcg aacagggcca cgccgaggag
     6901 gatgagtgtt gcggtgcgga tgcgatgcga catgaccagg ctcctccagg tgaaggggcg
     6961 cgggtgcgtc gcccgcgcga aactccagcg actccgaatc gtttgcgagt cgggcggaat
     7021 tctagggttc gggcgtgacg gagcgattgc ggcggcggga cgagttcgtt acgtcgggat
     7081 ctgaggcgga ggagcggctc gcggcaggcg cgagcggacg gcaaggcgct ttgggagcta
     7141 gatcttcatc gcggcgagca tcgcttcgac cgccgcgcgc atgcccacct cgatcgcgag
     7201 cgagacgatg tgatgcccga tggagagctc cgtgaagggg aaggcgcgca gcagggggcc
     7261 gacgttcgcg gtggtgatgc cgtggccggc gtggacctcg agcccgaccg cgtggaaggc
     7321 gcgcgcggcg agcgcgatgc gatcgagctc ggcggcgagc gccgcttctc cccgcgcgtt
     7381 cgcgtaggcg ccagtgtgga tctcgaccat cgcggccccg cagtcgcgcg cgaccggcgc
     7441 cagcgcgggc tcggggtcga ggaaaagact cagcgtgatc tcccgctccc gcagtcgcgg
     7501 cgcggcgcgg cgcaccgcgg gcgcgtggcg catcaggtcc agcccgccct cggtggtcag
     7561 ctcctcgcgc cgttcgggca ccagggtgga gcgctcggga cggagatcga gcgcgatcgc
     7621 gagcatctcc tcggtcagcg ccatctcgag attgagctcg ccgcggcgca tggcgcgcag
     7681 gcgccgcacg tcgtcgtcct ggatgtggcg acggtcttcg cgcaggtgca ccgtgatccc
     7741 cgcggcgccg ccatccaatg ccagcgcggc ggcgcgggcg ggatcgggct cggtcccgcc
     7801 gcgcgcctcg cgaagcgtgg cgacgtggtc cacgttgacc gagagcgcgc aggtcacagc
     7861 acgatctccg atgccggaac caggtccacg ccctgggccc gcagcgcgtc gcgctccttc
     7921 tccagcacgc cgaggatcac cgagctggga tgcagcgtga cgaccagcgc cccgcgctgc
     7981 acggccgtgc
//
LOCUS       DASSLZ010000198        12410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1594689, whole genome shotgun sequence.
ACCESSION   DASSLZ010000198 DASSLZ010000000
VERSION     DASSLZ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12410
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594689"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..615)
                     /locus_tag="VFM00_07640"
     CDS             complement(10..615)
                     /locus_tag="VFM00_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498158.1"
                     /translation="MNENRNTQDSQHDDRLFFLKAGLIAAPLLIALAALGCGEQKEKA
                     TAELAHRDAAVVPVEQGASAGQGAAATTTALTSGGVVTGVAETTPNADETLPPDIVAT
                     GPESVVIPGSVVTIAATGSTDVTSVMLTDRAGEKTPFTYDSESNLWQASYRVPLRVGT
                     EKLALAVTATTEANRYKRVWVFLRLRDEKATPAEAAPDSAQ"
     gene            919..2019
                     /locus_tag="VFM00_07645"
     CDS             919..2019
                     /locus_tag="VFM00_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498159.1"
                     /translation="MGDTETCDTPRFGAAGAALAVIIGLATSCAASNGHRVLWTRADH
                     VYVALADGAAVVEGDSARFEERGKPLGRGRVAEVVRGEVAVVAVPPGAFERVNKLDRI
                     RVAFTRAGASRPPSLRVALPAGGRRNLAVPCAAGSDGLPVLPQDLRGYVLNERERSER
                     PLRLRPDPHAVPPEGWPDTLIVSFFESVTDEEIALERGDVDVAVFWPGELSRRMREDA
                     RWKGAPRGLRSRGVIAITGADIVPAGLDSATVRALDDELFRGDLAWTGAFVPASERGP
                     GRLGIDESLPGFAAIREWLTRRLGTPRGGIVGRIAYVDAPSDSPPAPGFQPLYRMTCP
                     VVSGPAWRPVIERIGSGVFADMIPCTAAAPPR"
     gene            2016..3725
                     /locus_tag="VFM00_07650"
     CDS             2016..3725
                     /locus_tag="VFM00_07650"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7498160.1"
                     /translation="MNSLRVRLIVGFALVALVPLAIAIVLLTDRIERMVRDQAADRVT
                     GALQAVTSQLVEDERRIVEKAQLLAQDPELRRLYLVRGAGARDLADFLEERRYLLGLD
                     FLRVTDVSGALRADAADAVSRSASPARPARYGPPPAGRRGISVRRLESDSGLVMVSAA
                     PIQYLGATTGHVECGLALDGTFLDRWRNRSGVEFALYDAQGRAAGGTSLLAMTSWGAG
                     EGRYRMVGTTGADLYETVPIAIGDPPYARLLGAAATSEAEAAITALQRASAILGLVAL
                     GVAVVLGFAWSRQISRPVERLAGYSEKLARGEWDQPLELKSVRELETLVVALDRMRRD
                     LLSYRERLVVTERQAAWSQMARKVAHEVKNPLTPIAISIADLKRSYEAKRPDFAAILD
                     QAVRTIGEEVETLKRLLQEFSDFGRFPPPRIEPCRWSGLAADLETLYGDEIRTGRLAV
                     SPEGRGLVFPADAGQVRQALVNLVRNGLDATAGGGRVLVGAAAEDTTAVITVADDGPG
                     LSQEQRARLFTPGFTTKPEGSGLGLVIVERIVNDHGGNIDVRTAPGQGTTFTLRLPLK
                     REA"
     gene            3728..5086
                     /locus_tag="VFM00_07655"
     CDS             3728..5086
                     /locus_tag="VFM00_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7498161.1"
                     /translation="MPGVLIVDDEANIRSSLEGALGREGYQVECAATAAEARAKLRDS
                     FDYILLDVWLPDGSGLDLLRESLAADPDAVVIMMSGHATIDAAVQATRLGAFDFLEKP
                     LSLERLLVLLRNAGAAVALQSENRRLTRGWAKPILGRSPAIRRLLGEIERAGAGAARV
                     LIQGENGTGKELVARALHAAGPREGRPFVAVNCAAIPEELIESELFGHERGAFTGATQ
                     ARKGRFEEAHGGTLFLDEVGDLSARAQTKLLRVLQEGELTRVGGNRAIRVDVRVIAAT
                     NQDLAARVRAGRFREDLYFRLAVVPILVPPLRERAEDVPLLVERFVAETAAEAGVKPR
                     SFAPAAVLALTRYTFPGNVRELRNIVERLLIMAPGERVEADAVRSILPREPEEPGPPD
                     GAGAGRLADRVRDFERSAIQAALAAEGGSMTRAAARLGLERSHLYKKMKSLGWTPDED
                     AR"
     gene            5210..5719
                     /locus_tag="VFM00_07660"
     CDS             5210..5719
                     /locus_tag="VFM00_07660"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET7498162.1"
                     /translation="MKRGSPLLAAAFLALLLLTLAPIAHAAAPARGAVAPSFTLPTAT
                     GSVSLDSLQGNVVLVDFWASWCVPCKSSFPWMTAMHQRYGPKGLAIVAINVDKSRKSA
                     QEFLEKHPAPFIVAYDPDGKTAKAYHVWGMPTSYLVGHDRAILFSRSGFDPKHAKDVE
                     AMIAQACAR"
     gene            5716..5952
                     /locus_tag="VFM00_07665"
     CDS             5716..5952
                     /locus_tag="VFM00_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HET7498163.1"
                     /translation="MSGRVRRLLARTFLAAAAAASAGCAAMKVEPWDRDLLAEKKMSF
                     TPAPLESAIDDHVYFSKEGSTGGQGVGGGGCGCN"
     gene            5975..7354
                     /locus_tag="VFM00_07670"
     CDS             5975..7354
                     /locus_tag="VFM00_07670"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HET7498164.1"
                     /translation="MTPPPGGTPRRVSALRARLGAAACLLVAAGARSAAAQIPDSTTT
                     PDTSWNPDSTRAPVAASSPSYRWQFDGAGLLYSEKGRAKVIEPQARITRLFPDGQTLS
                     ATLGLDVITGASPTGAIPTTTVQTTTTPSGNVQTATANEVPTHPFNDLREGLDLEWTK
                     PFGGWLTPSLGTHFSREKDYRSVGGTAKLSVAMMRKLTTVTVGAGYNEDRVDPVGGTR
                     APLADGSVLLGTGANPKRVRSALAGVSRVLTRRWLFGVTGSRSTEHGYLTDPYKVISV
                     LDTTGAPTSDVVTESRPTSRTRYDVLASSVYHLTQNILYASYRYYWDDWGVTSHTADA
                     RYRVELSNQSFFQPHIRYYFQTRANFFQFGLPQGAPLPEFATSDARLGDLRTITLGAT
                     YGFHPHAGPGEVTLRAEYLRQWGKGSGGDQDGGEFEGEGAPAASSGRIDFIPPESIGS
                     IVVGYSIPF"
     gene            7362..8285
                     /locus_tag="VFM00_07675"
     CDS             7362..8285
                     /locus_tag="VFM00_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET7498165.1"
                     /translation="MSPAPTSRPGAPPIVERAGDVFIGRFTAMASPCEVLVDTDDRAE
                     AGSLAERAADEARRVEAKFSRYREDSVVGRLNRAQGAATAVDEETARLLDYAAACWEE
                     SNGLFDVTTGALRRAWTFDGSGRIPAEAAVRAALRDVGWARVRWDGSSLMMPAGMEID
                     LGGIGKEYAVDGAAALLMERTEAPLLVNFGGDLFASGPRRRGAPWIVGVDDPARTGAS
                     ALYRVDLLGGGLATTGDARRFVIRDGKRLGHILDPRTGWPVEDPPASVTVLARSCLEA
                     GTLSTLAYLHGRGAEAFLRGQGVEFRVVPGA"
     gene            8367..9605
                     /gene="purT"
                     /locus_tag="VFM00_07680"
     CDS             8367..9605
                     /gene="purT"
                     /locus_tag="VFM00_07680"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HET7498166.1"
                     /translation="MRIGTPLSPGAVKVMLLGSGELGKEVVIALQRYGAEVIAVDRYP
                     HAPAHHLAHRWHAIDMADPRALRDVIARERPHLVVPEIEALATDELARVEADGLAEVI
                     PTARAVQLTMNREGIRRLAAETLSLPTSRYRFAQSAAEVREAAAALGYPVVVKPVMSS
                     SGKGQSVVHDAGEVDAAWEYAESAGRVKHGRVIVEEKIEFEYEITLLTVRALGPDGQV
                     ATSFCEPVGHVQVKGDYVESWQPQPMHPAALERAREVARAVTGALGGRGVFGVELFVR
                     GDQVWFSEVSPRPHDTGLVTMATQAQSEFELHARAILGLPVSTELQAPGASAVIYGGM
                     DAAGIAFEGLDEALRVPRTAVRLFGKPESFLRRRMGVATAWGGDVADARERAKRCAGL
                     VRPVRAPRDASVEESSAAPS"
     gene            complement(9637..10320)
                     /locus_tag="VFM00_07685"
     CDS             complement(9637..10320)
                     /locus_tag="VFM00_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7498167.1"
                     /translation="MIRSMRKVATLALAGALVLGGVPGCSSLSKEQKGAIIGAAAGAA
                     GGAAVGKATGSTARGAIIGAVVGGAAGAVIGHKMDQQARELEQNIPGAIVERVGEGIQ
                     ITFESGLLFDFDSDAIRGTARRNLDELARSLEKYDETNLLIVGHTDAVGTDSYNLDLS
                     DRRAEAAARYLRSQGVDRDIETVGRGEREPVASNDTESGRSRNRRVEVAIYASPEMVD
                     RARREARAE"
     gene            complement(10384..10995)
                     /locus_tag="VFM00_07690"
     CDS             complement(10384..10995)
                     /locus_tag="VFM00_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET7498168.1"
                     /translation="MATRALNAQPTAPRPLATPTDLGPDDVRAVVEAVNPLIADSLAL
                     YVKTKNFHWHVSGPHFRDYHLLFDEQAEVVLESVDILAERLRKIGGSTIRSIGHVASL
                     QNIADDEEEYVTPQEMVRRLLDDNRAMAERQRAAIETCDDHHDTPTGNLLQDILDQTE
                     RRVWFLFEVSQTGEPASESEESQTAGPRATARTRPPGGRSGRR"
     gene            complement(11097..>12410)
                     /locus_tag="VFM00_07695"
     CDS             complement(11097..>12410)
                     /locus_tag="VFM00_07695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7498169.1"
                     /translation="SPRLRLPDLLATRRGRLAAFFLLYFTEGIPAGFTGTAVATQMRR
                     QGLDPAAIGGFIAALYLPWAIKWAFGPFVDVLTVERWGRRRFWILLTQAMMAVSLLAA
                     APVEFRGHLGLFTAIILVHNIFAATQDVAIDSLAVNTLHGGERGLANGLMFAGQYLGN
                     AAGGAGVLLLTPLVGFKTTFLIVAGTIALVTLLVPVPMREAAGPPRAPRPGRLLAAVA
                     REIARFARDAARAFTGSRPAFLAIFLALLPMGAYALSLSLQSNLAVELGLTDTQIGIL
                     GLWSTALSAGFCVLGGWLSDRFGRRRTLALFVAGTTLPTFAMALAMRHYGWILPVDPS
                     MPGRPPVPHELVRLFWVLTLGYAVFQGLMYGVGTAIYMDVTTPAVAATQFTAYMALCN
                     LVYSYTSGWQGHALVRWGYPATLALDGAFGLLSLVLLPFMGRPRR"
BASE COUNT         1740 a         4456 c         4420 g         1794 t
ORIGIN      
        1 cgctgcgctt cactgcgccg aatcgggagc cgcctccgcc ggcgtagcct tctcatcgcg
       61 caggcgcagg aagacccaca cccgcttgta gcggttcgcc tccgtcgtcg cggtgaccgc
      121 gagcgcgagc ttctcggtgc ccacgcgcag cggcacgcga taggacgcct gccagaggtt
      181 cgactcggag tcgtacgtga acggcgtctt ctcgccggct cggtcggtca acatcaccga
      241 ggtcacgtcc gtggatccgg tcgccgcgat cgtcaccacg ctgccgggaa tcacgaccga
      301 ttccggtcca gtggcgacga tgtccggagg cagcgtctcg tccgcgttcg gggtggtctc
      361 ggcgacaccg gtcacgacgc cgcccgacgt gagcgcggtc gttgtggcgg cggcgccctg
      421 acccgcggag gcaccctgct ccacgggaac gacggccgca tcgcgatgcg cgagctcggc
      481 ggtcgccttc tccttctgct ccccgcagcc cagggccgcg agggcgatca gaagaggcgc
      541 cgcgatcagt cccgccttca ggaagaagag cctgtcgtcg tgctgcgaat cctgggtatt
      601 cctgttctcg ttcatggtcc tgtctccaca tccggcttct gatgcggcgt cgaggagcgg
      661 cgccggggcc gcggctcgat cgcctggcgt gggcggctcc ccgggccagg accgtgagcg
      721 gcgcgcgggg cgccgctcga aagggttcta ttcaaccgcc gtgccgcgcg cgtaagtgac
      781 tggccagttg ccagatacct gaagagcagg gtcccggtgg cggggttcgc tgtgtcagcc
      841 ttgacacgca cctggagcgg gagcagtctg cggatgtccc caaccatcaa cgaggcccgg
      901 acttgggagg cgcgtgtcat gggtgacacg gaaacgtgtg acacgccgcg cttcggcgcg
      961 gccggcgcgg cgctggcggt catcatcggc ctggcgacct cgtgcgcggc gtcgaacgga
     1021 catcgcgtcc tttggacccg cgccgaccac gtttacgtgg cgctggccga cggcgcggcg
     1081 gtcgtggaag gcgactcggc tcgtttcgag gagcgcggga agccgctcgg acggggacgc
     1141 gtggccgaag tcgtgcgcgg cgaggtcgcg gtcgtggccg ttccaccggg cgcgttcgag
     1201 cgcgtgaaca agctcgatcg gatccgcgtc gccttcacgc gcgccggggc ctcccggccg
     1261 ccgtcgttgc gcgtcgccct gcccgcgggc gggcgccgca atctggccgt cccctgcgcg
     1321 gccgggtccg acggtctgcc ggtcctgccc caggaccttc gcggctacgt gttgaacgag
     1381 cgagagcggt cggagcggcc gcttcgcctc cgtcctgatc cccacgcggt gccgccggag
     1441 ggctggcccg acaccttgat cgtctccttc ttcgagagcg tcaccgacga agagatcgcg
     1501 ctcgagcgcg gcgacgtgga cgtcgccgtc ttctggccgg gcgagctgtc gcgtcggatg
     1561 cgcgaggacg cgcgctggaa gggcgcgcct cgcggtctgc gaagccgcgg ggtgatcgcg
     1621 atcacgggcg ccgacatcgt ccccgccggg ctcgactcgg cgaccgtgcg cgccctggac
     1681 gacgagttgt tccgcggcga tctggcttgg accggcgctt tcgtcccggc ttcggagcgg
     1741 ggcccgggcc gcctggggat cgacgagagc cttcccggtt tcgccgcgat ccgggagtgg
     1801 ctgacgcgcc gcctggggac tcctcgaggc ggcatcgtgg gtcggatcgc gtacgtcgac
     1861 gcgccgtccg attcgccccc ggctccggga ttccagccgc tctaccggat gacctgcccc
     1921 gtggtgagcg ggcccgcgtg gcgtccggtc atcgagcgga tcggttccgg cgtcttcgcc
     1981 gacatgatcc cctgcacggc cgcggcgccg ccgcggtgaa ctccctccgc gtccgcctga
     2041 tcgtggggtt cgccctcgtg gcgctggtcc ccctcgcgat cgcgatcgtg ctgctcaccg
     2101 accggatcga gcgcatggtg cgcgatcagg cggcggatcg cgtcacgggc gcgctccagg
     2161 ccgtcacgtc gcagctggtc gaggacgagc gccgcatcgt cgagaaggcc cagctcctgg
     2221 cgcaggaccc ggagctgcga cgtctctacc tggtccgcgg cgcgggcgct cgcgatcttg
     2281 ccgacttcct ggaagagcgc cgctaccttc tggggctcga cttcctgcgc gtcaccgacg
     2341 tgtcgggggc tctccgcgcc gatgccgcgg acgcggtctc gcgctccgcg tcccccgcgc
     2401 gcccggcacg ctacggcccg ccgcctgccg gacgccgcgg catctcggta cggcgcctgg
     2461 agagcgattc cggcctcgtg atggtgtccg cggctccgat ccagtacctc ggcgcgacca
     2521 cggggcacgt cgaatgcggc cttgccctcg acggcacctt tctcgaccgg tggcgcaacc
     2581 gtagcggggt ggagttcgcc ctgtacgacg cgcagggacg ggcggccggg gggacctccc
     2641 tgctcgcgat gacctcctgg ggcgccggcg agggccggta ccggatggtc ggcaccaccg
     2701 gcgccgatct ctacgaaacg gtaccgatcg ccatcggcga tcccccctat gctcgcctcc
     2761 tcggcgcggc ggcgacgagc gaggccgagg cggcgatcac ggcactgcag cgcgcctcgg
     2821 ccatcctcgg cctcgtcgcg ctcggcgtgg cggtcgtcct cggcttcgca tggtcgcggc
     2881 agatctcacg ccccgtcgag cggctcgccg gatactccga gaagctcgcg cgcggcgaat
     2941 gggaccagcc gctcgagctg aagagcgtcc gcgagctgga gacgctggtc gtggcgctgg
     3001 accggatgcg gcgcgatctc ctgtcctacc gggagcgcct cgtcgtgacc gagcggcagg
     3061 cggcgtggag ccagatggcg cgcaaggtgg cgcacgaggt gaagaatccg ctcaccccga
     3121 tcgcgatctc gatcgcggac ctgaagcgca gctacgaggc caagcgcccc gattttgccg
     3181 cgatcctgga ccaggcggtg cgcacgatcg gcgaagaggt ggagaccctg aagcgcctgc
     3241 tccaggagtt ctccgacttc ggccgcttcc ccccgccgcg catcgagccc tgccgctggt
     3301 cgggtctcgc ggccgacctc gagaccctct acggcgacga gatccgcacc ggaagactcg
     3361 cggtgtcgcc cgaggggcgc gggctcgtct tccccgccga cgcggggcag gtccgccagg
     3421 cgctcgtgaa tctggtccgg aacgggctgg acgcgacggc cggcggtgga cgcgtcctgg
     3481 tcggcgcggc cgccgaggat acgaccgcgg tgatcacggt cgccgacgac ggtcccggcc
     3541 tgagccagga gcagcgggcg cgcctcttca cccccggctt caccaccaag ccggagggaa
     3601 gcggcctggg acttgtgatc gtcgagcgga tcgtgaacga ccacggcggg aacatcgacg
     3661 tgcgcacagc accgggccag ggcaccacct tcaccctccg tctccccttg aagcgcgagg
     3721 cctgacgatg cccggcgtgc tgatcgtcga cgacgaagcg aacatccgtt ccagcctcga
     3781 gggggcgctg ggccgcgagg gctaccaggt ggagtgcgcg gcaaccgcgg cggaggcgcg
     3841 ggccaagctc cgcgattcct tcgactacat cctcctcgac gtctggctgc ccgacgggag
     3901 cggtctcgat cttctccgcg agtcgctcgc cgccgacccg gacgcggtcg tgatcatgat
     3961 gtcggggcac gccacgatcg acgccgccgt gcaggcgacg aggctgggcg cgttcgattt
     4021 cctcgagaag ccgctctccc tggagcgcct cctggtcctg ctccggaacg ccggcgccgc
     4081 ggtcgcgctc cagtcggaga accgccggct cacgagggga tgggccaagc cgatcctcgg
     4141 acgctccccc gcgatccgcc ggctcctcgg cgagatcgag cgcgccggcg cgggggcggc
     4201 gcgcgtgttg atccaggggg agaacggcac gggcaaggag ctggtcgcgc gcgcgctcca
     4261 cgccgcggga ccgcgggagg gccgtccttt cgtcgccgtg aactgcgccg ccattcccga
     4321 ggagctgatc gagtcggagc tgttcgggca cgagcgcggc gccttcaccg gcgcgacgca
     4381 ggcgcggaag ggacgcttcg aggaggcgca cggcggcacg ctcttcctcg acgaggtcgg
     4441 cgatctcagc gcgcgcgcgc agaccaagct cctccgcgtg ctccaggaag gggagctgac
     4501 gcgcgtcgga ggcaaccgcg ccatccgcgt ggacgtgcgg gtgatcgccg cgacgaacca
     4561 ggatctcgcg gcgcgggttc gcgccggccg cttccgcgag gacctctatt tccgtctcgc
     4621 ggtggttccg atcctggtgc cgccgctgcg cgagcgcgcc gaggacgttc cgctcctcgt
     4681 ggagcgcttc gtcgccgaga ccgcggcgga agcgggcgtg aagccgcgct cgttcgcccc
     4741 ggccgccgtc ctggcgctca cacgctacac gtttcccggg aacgtgcgcg agttgcggaa
     4801 catcgtggag cgcctcctca tcatggcccc gggcgagcgc gtcgaggccg acgcggtgcg
     4861 ctcgatcctt ccgcgcgagc cggaggaacc cggcccgccc gacggcgcgg gcgcgggaag
     4921 gctggccgac cgcgtgcgcg actttgagcg aagcgccatc caggcggcgc tcgcggccga
     4981 gggcggaagc atgacgcgcg ccgccgcgcg attgggattg gagcgcagcc atctctacaa
     5041 gaagatgaaa tcgttgggat ggacgcccga cgaggacgcg cgctgaacgc gggaaccgcc
     5101 gcccggcccg aggggtaccg agggcaggac agccggacgc ccggaccatc cgggtagagc
     5161 gaaggtcccc cccctttcgc aaggctcgac ccaacggagg gatgcggctt tgaaacgtgg
     5221 ttcacccctt ctggcagccg ctttcctggc cctgcttctg ctcacgctcg cacccatcgc
     5281 ccacgccgcc gcgcccgcgc gcggcgcggt cgctccgtcg ttcacccttc cgaccgccac
     5341 cggctcggtc tcgctcgact cgctccaggg aaacgtcgtg ctggtcgact tctgggcgtc
     5401 gtggtgcgtt ccctgcaaga gctcctttcc gtggatgacc gccatgcacc agcgctacgg
     5461 cccgaagggg ctcgccatcg tcgcgatcaa cgtggacaag tcgcggaagt cggcgcagga
     5521 attcctcgag aaacacccgg ctccgttcat cgtggcgtac gaccccgacg gaaagaccgc
     5581 gaaggcatac cacgtctggg gcatgcccac gagctacctc gtcggccacg accgcgcgat
     5641 cctcttctcg cgttccggct tcgatcccaa gcacgcgaag gacgtcgagg cgatgatcgc
     5701 ccaggcgtgc gcccgatgag cgggcgcgtt cggcggctcc tcgcccgaac gttcctcgcc
     5761 gccgccgccg ccgcgtcggc cggctgcgcg gcgatgaagg tggagccgtg ggaccgcgac
     5821 ctcctcgccg agaagaagat gagcttcacg cccgcgcctc tcgagtccgc catcgacgac
     5881 cacgtctact tcagcaagga aggatcgacc ggcggccagg gggtcggcgg aggcggctgc
     5941 ggatgcaatt gagcgacgtg cccgggtgcg cgcgatgacg ccacctccgg gagggacccc
     6001 ccggcgcgtc tcggcactcc gcgcgcgcct cggcgccgcg gcgtgtcttc tcgtggccgc
     6061 gggcgcgcga agcgctgcgg cgcagatccc cgattcgacc accacgcccg acaccagctg
     6121 gaatcccgat tcgacgcggg cgccggtcgc ggcgtcatca ccctcctacc gctggcagtt
     6181 cgacggcgcg ggccttctct acagcgagaa ggggcgcgcc aaggtgatcg agccgcaggc
     6241 gcgcatcacg cgcctcttcc ccgacgggca gaccctctcg gccacgctcg gactcgacgt
     6301 catcaccggg gcctcgccca cgggcgcgat ccccacgacc accgtccaga cgacgaccac
     6361 gccctccggc aacgtccaga cggcgacggc gaacgaggtc ccgacgcacc ccttcaacga
     6421 cctgcgcgag gggctggacc tcgagtggac gaagcccttc ggcggctggc tcaccccgtc
     6481 gctcggaacg cacttctcgc gggagaagga ctaccgctcg gtgggcggca ccgcgaagct
     6541 ctcggtcgcg atgatgcgca agctcacgac ggtcacggtc ggtgcgggct acaacgaaga
     6601 tcgcgtggac ccggtgggcg ggacgcgcgc gcccctcgcc gacggcagcg tcctcctcgg
     6661 cacgggagcg aacccgaagc gggttcgcag cgcgctcgcg ggggtctcgc gcgtgctcac
     6721 gcgccgctgg ctcttcgggg tgaccgggtc gcgctcgacc gagcacggct acctcacgga
     6781 tccgtacaag gtgatcagcg tgctcgacac gacgggcgcg ccgacgagcg acgtcgtgac
     6841 ggaatcgcgc cccacgtcgc ggacgcgcta cgacgtgctc gccagctcgg tctatcacct
     6901 gacgcagaac atcctgtacg cctcctatcg ctactactgg gacgactggg gcgtcacgtc
     6961 gcacacggcc gacgcgcgct atcgcgtgga gctgtcgaac cagagcttct tccagccgca
     7021 catccgctac tacttccaga cccgcgccaa cttcttccaa ttcgggcttc cccagggcgc
     7081 accgcttccg gagttcgcga cgagcgacgc gcgcctgggc gacctccgga cgatcacgct
     7141 cggcgccacg tacggattcc acccgcacgc gggcccgggc gaggtgaccc tgcgggccga
     7201 gtatctccgg cagtggggca agggatcggg tggcgatcag gacggcggcg agttcgaggg
     7261 cgaaggggcc ccggccgctt cttccggacg gatcgacttc atcccgccgg agagcatcgg
     7321 ctccatcgtg gtcgggtact cgattccctt ctaggcatcg catgagcccg gccccaacgt
     7381 cacgacccgg cgcgcctccc atcgtggagc gcgccggaga cgttttcatc ggacgcttca
     7441 ccgccatggc gagtccgtgc gaggtcctgg tcgataccga cgaccgggcc gaggcgggct
     7501 cgctcgccga acgggccgcg gacgaggcgc gccgcgtcga ggccaagttc agccgctatc
     7561 gcgaggacag cgtggtcggg cgcttgaacc gcgcccaggg ggcggcgacg gcggtggacg
     7621 aggagacggc gcgcctcctc gactacgccg ccgcgtgctg ggaggaatcg aacggcctct
     7681 tcgacgtgac gaccggggcc ctgcgccggg cctggacgtt cgacgggagc ggccggatcc
     7741 ctgccgaagc cgccgtccgc gcggcactcc gcgacgtggg atgggcgcgc gtccgttggg
     7801 atgggtcgtc gctcatgatg ccggccggga tggagatcga cctcgggggg atcggcaagg
     7861 aatacgccgt cgacggcgcc gcggccctcc tcatggagcg gaccgaagcc ccgctcctcg
     7921 tgaacttcgg aggcgatctc ttcgcgagcg ggccgaggcg ccgcggcgcc ccgtggatcg
     7981 tcggcgtgga cgaccccgcg cggaccggcg cctcggcgct ctatcgcgtc gacctccttg
     8041 gcggcggcct ggccacgacg ggcgacgccc ggcgcttcgt gatccgggac gggaagcgcc
     8101 tggggcacat cctcgatccg cgcacgggat ggcccgtcga ggaccctccc gcctcggtga
     8161 ccgtgctggc gcgcagctgc ctggaggcgg ggaccctctc gacgctcgcc tatctgcacg
     8221 gccgcggcgc cgaggcgttc ctccgcggcc agggagtgga gttccgcgtc gttcccggcg
     8281 cttgaacgcg gggccgcgcc ccgctaggct ccgcgcctcg aatcgccgca tcccgccgtc
     8341 acgcacccgg tcccggaggc acgccgatgc gcatcggaac tcccctttcg cccggagccg
     8401 tcaaggtgat gctcctcggc tcgggcgagc tgggcaagga agtcgtcatc gccctgcagc
     8461 gctacggcgc cgaagtgatc gcggtcgacc gctacccgca cgcccccgcc catcacctgg
     8521 cgcaccgatg gcatgcgatc gacatggccg acccgcgcgc gctccgcgac gtgatcgcgc
     8581 gggaacgccc ccacctcgtc gttcccgaga tcgaagcgct ggccacggac gagctcgcgc
     8641 gcgtcgaggc cgacgggctc gcggaggtga ttcccaccgc gcgcgccgtc cagctcacga
     8701 tgaaccgcga agggatccgc cgcctcgccg ccgagacgct ttccctgccc acctcgcgtt
     8761 accgcttcgc ccagagcgcg gcggaggtcc gggaggccgc ggcggctctc ggctatccgg
     8821 tcgtcgtgaa gccggtcatg tcctcgtcgg gcaaggggca gtcggtggtg cacgacgccg
     8881 gcgaggtgga cgccgcgtgg gaatacgccg agagcgcggg gcgcgtgaag cacggccgcg
     8941 tgatcgtgga agaaaagatc gagttcgagt acgagatcac cctgctcacc gtgcgcgcgc
     9001 tcggccccga cggccaggtc gcgaccagct tctgcgagcc agtcgggcac gtgcaggtga
     9061 agggcgatta cgtggagagc tggcagccgc agccgatgca cccggccgcc ctggagcggg
     9121 cccgcgaggt cgcccgtgcc gtcaccgggg ccctgggagg gcgcggcgtg ttcggcgtcg
     9181 agctgttcgt ccgcggcgac caggtctggt tcagcgaagt gagccccagg ccccacgaca
     9241 cggggctcgt caccatggcg acccaggcgc agagcgagtt cgagctccac gcgcgcgcca
     9301 tcctcgggct gcccgtgagc acggagctcc aggcacccgg cgcgagcgcc gtgatctacg
     9361 gcggcatgga cgcggccggg atcgcgttcg aggggctgga cgaggcgctg cgcgtgcccc
     9421 gcaccgcggt gcgtctcttt ggaaagccgg agtcgttcct ccgccgccgc atgggcgtgg
     9481 cgacggcctg gggcggcgac gtcgccgacg cgcgcgagcg cgcgaagcgc tgcgccggcc
     9541 tggtccggcc ggtgcgcgct ccgcgcgatg cttccgtcga ggaatcctcg gcggcgcctt
     9601 cctgacgcgc gccgcccctg ccgcgcccgg cggtggctac tcggcccggg cctcgcgccg
     9661 cgcccggtcc accatctccg ggctcgcgta gatcgccacc tcgacgcggc gattccggct
     9721 ccgccccgat tccgtgtcgt tcgacgccac cggctcccgc tcgccgcgac ccaccgtctc
     9781 gatgtcccgg tcgacgccct gcgagcgcag gtagcgcgcc gcggcttcgg cccgccggtc
     9841 cgagagatcc aggttgtagc tgtcggtgcc gacggcgtcg gtgtggccga cgatcaggag
     9901 attggtctcg tcgtacttct ccaggctgcg cgctagctcg tccaggttgc ggcgcgccgt
     9961 gccgcgaatg gcgtcggaat cgaagtcgaa gagcaggccg gattcgaacg tgatctgaat
    10021 gccctctccg acgcgctcca cgatggcgcc cgggatgttc tgctccagct ccctggcctg
    10081 ctggtccatc ttgtggccga tgaccgcgcc ggccgcaccg ccgacgaccg cgccgatgat
    10141 ggcgccgcgc gccgtcgagc cggtcgcctt cccgaccgcc gcaccgcccg cggcacccgc
    10201 cgcggcgccg atgatcgcgc ccttctgctc tttgctgagc gacgagcagc cggggacgcc
    10261 gcccagaacg agcgcgccgg ccagcgccaa cgtcgccact ttccgcatgg aacgaatcat
    10321 gaagtgcctc cactccggcc cgcgaccctc gccgcgcgcc ggattcagtt tgggttcagg
    10381 gaattatcgg cggccggatc gtccgcccgg cggacgggtc cgcgccgtcg cgcgcgggcc
    10441 cgctgtctgg gattcctccg actcggaggc gggctcgccg gtctggctca cttcgaacag
    10501 gaaccatacg cggcgctcgg tctggtccag gatgtcctgg agcaggttgc cggtcggcgt
    10561 gtcgtgatgg tcgtcgcagg tctcgatggc cgcgcgctgg cgctcggcca tcgcgcggtt
    10621 gtcgtcgagc agccgccgga ccatttcctg cggcgtgacg tactcctcct cgtcgtcggc
    10681 gatgttctgc agggaggcga cgtggccgat gctccggatg gtgctgccgc cgatcttccg
    10741 caggcgctcc gccaggatgt ccaccgattc cagcacgact tcggcctgct cgtcgaagag
    10801 gagatggtag tcacggaaat gcggcccgct cacgtgccag tggaagttct tggtcttcac
    10861 gtacagcgcg agcgagtcgg cgatgagcgg attgacggcc tccactacgg cgcggacgtc
    10921 gtccggtccg aggtcggtgg gcgtcgccag cggccggggg gccgtgggct gggcgttcaa
    10981 ggcgcgggtg gccatggatt ctccttccgg tgatgaaccg ggattcgttt cgattcgggg
    11041 tcccctgctc cgggaccgcg gggcgcggcc ccatcggccg cgcagcgcgc ccctgattat
    11101 cgtcgcgggc gtcccatgaa gggaagcagg acgagcgaga gcagcccgaa agcgccgtcc
    11161 agcgccaggg tagcgggata gccccaccgc accagcgcgt gcccctgcca ccccgaggtg
    11221 taagagtaga cgaggttgca tagggccatg tacgcagtaa attgcgtcgc ggcgaccgcc
    11281 ggcgtggtca cgtccatgta gatcgcggtg ccgaccccgt acatgagtcc ctggaagacg
    11341 gcgtacccca gcgtcagcac ccagaagagc cggaccagct cgtgcggcac cggaggcctt
    11401 cccggcatcg agggatctac ggggaggatc cagccgtagt ggcgcatcgc cagggccatc
    11461 gcgaaggtcg ggagcgtggt gccggccacg aacagagcga gggtgcgccg ccgcccgaag
    11521 cgatccgaga gccaccctcc cagcacacag aaacccgccg agagcgccgt gctccagagg
    11581 ccgaggatcc cgatctgcgt gtccgtcagc cccagctcca ccgcgagatt ggactgcagc
    11641 gacagggaga gggcgtacgc gcccatgggc aggagcgcca ggaagatcgc gaggaacgcg
    11701 ggccggctcc cggtgaaggc acgggccgcg tcccgcgcga agcgcgcgat ctcgcgggcg
    11761 acggcggcca ggaggcgccc cgggcggggc gcgcgcggcg gccccgccgc ttcccgcatc
    11821 ggcaccggca ccagcaacgt cacgagcgcg atcgttccgg ccacgatcag gaaggtggtc
    11881 ttgaacccca cgagcggcgt gagcaggagg acgccggcgc cgccggcggc gtttcccagg
    11941 tactgccccg cgaacatgag gccgttcgcc agcccgcgct cgccgccgtg aagcgtgttc
    12001 accgccaacg agtcgatcgc gacgtcctgc gtggccgcga agatgttgtg aaccaggatg
    12061 atcgccgtga agagcccgag atggccccgg aactctacgg gggccgcggc cagaaggctc
    12121 accgccatca tcgcctgggt gaggaggatc cagaaccggc gccgccccca ccgctccacg
    12181 gtgaggacgt ccacgaaggg gccgaacgcc cacttgatcg cccacggaag gtagagggcg
    12241 gcgatgaacc cgccgatcgc cgccggatcc agcccctgcc ggcgcatctg cgtcgcgacg
    12301 gcggtcccgg tgaatcccgc ggggattccc tcggtgaagt agaggaggaa gaaggcggcc
    12361 agccgcccgc gccgcgtggc gaggagatcg ggaagtctca agcgggggga
//
LOCUS       DASSLZ010000199        14157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1595004, whole genome shotgun sequence.
ACCESSION   DASSLZ010000199 DASSLZ010000000
VERSION     DASSLZ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14157
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595004"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VFM00_07700"
     CDS             <1..520
                     /locus_tag="VFM00_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HET7498170.1"
                     /translation="IAPGDVVLGLPSAGLHTNGYSLARKILLEKLRLRVGDRFPGAAE
                     TVGEVLLAPHLYYGPDVRIAREAGTIRGMAHITGGGIPGNLVRILPEGVRAEIRMGSW
                     PVPHVFRTLAEAGGVPDAEAYEVWNMGLGLLLVTPAGDADRIEAALAGAGRACFRAGA
                     IAAGPREVRLIP"
     gene            557..1615
                     /locus_tag="VFM00_07705"
     CDS             557..1615
                     /locus_tag="VFM00_07705"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7498171.1"
                     /translation="MERIEAQADRPVPVPALAPGGPGPYAGALGLVGLSAPLRALCER
                     IAAHARSSATVLIRGETGSGKELVARALHRLSPRAGRAFLPHNFASIPDSLVESELFG
                     HARGAFTGAHSDRPGLFELADGGTLFLDEIGDASPSVQSRLLRVLQEGEVRRVGDGRA
                     RRVDVRIVAATHRDLAGEARAGRFRADLYYRLHVLALDVPPLRARRADVPVLAAHILE
                     SLARRDALAVRGVHPAALQLLLAHDWPGNVRELEAVLTRAAHSAGAGGVIEPEALGPE
                     VLGERPFLVRERHATLRGRTLAYEAELIRCALDVHRGNRSLAARSLGLSRQGLWKKMR
                     RLGVRAERDPIEAAEAAD"
     gene            1721..2734
                     /locus_tag="VFM00_07710"
     CDS             1721..2734
                     /locus_tag="VFM00_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7498172.1"
                     /translation="MAKRPLRIGVIGAGLVTQINHLPALKKRRDAEVVAVCDDDGEKA
                     RMVARHFGIARTLTDYETLLRSDEVVAVIVATPNHLHAPMVLAAMGYGKHVLCEKPPA
                     RNAAEAEQMAEAARRSGCTLMYAMNNRFRTDVQLLRGYMERREFGTIFYAKTGWLRRR
                     TAQRGPAWYQNKRSSGGGVLMDLGVQMLDLSLWLLGNPQVASVTATKYVTDPREDVED
                     TVAAFLVLDSGAALTLEVSWALLLEKNFPYLNLFGTHGAALLNPFRVHKELGGNLLDV
                     TPPLESKNVYKQSYEAELDHFIRCITQGERPVGTPEEGLQLMRVIDAIYQSAESRREV
                     RLH"
     gene            2739..4289
                     /gene="lnt"
                     /locus_tag="VFM00_07715"
     CDS             2739..4289
                     /gene="lnt"
                     /locus_tag="VFM00_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET7498173.1"
                     /translation="MRAPLAAAASGILASLAFPSASLWPLIFVALVPLLLVLASGSPG
                     PRDPEPASRGVRWAPWLMGTVFYALTFWWIVRLPVAAMTHPWLIYPGLLALGLYLGLY
                     VALFGWLVRFVRRRLGWPVLALAPFAWGVAEWLKSSGELGCPWGNLGTALAEHPAWIQ
                     GASLAGATGLSFWVVSVNALVAAILLARRWSSRIGLAMILAAAVGVPVVWGTARLKAP
                     APAPLVRVAIVQPNVGSHEKWVPALQDSVTARVERLTREAGMADPRPTVILWPETALP
                     YYVRVEPLKLQRLIALAKEVNRPILAGYPDAKLNAQGDVLTYNAAGLVLPAGAIVGQY
                     EKIHLVPFGERIPFQSLFPFLGSIDLGQAEWTPGTRPYVFRRAGPAFGVLICFESIFP
                     DHTRRYALEGAQYLVNITNDEWFGRTAGPIQHADLAILRSVELGMGIARAANTGVSML
                     IDPYGRVEARTPLFAEAVLVGTVKTGIGPTPFARWGDWISMLSLAVTLILVAVGWFRG
                     VDSRAKLR"
     gene            4366..5112
                     /locus_tag="VFM00_07720"
     CDS             4366..5112
                     /locus_tag="VFM00_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498174.1"
                     /translation="MPGSPESDATKPKSTSRARRPLHPGAWLFVCAALGVGFSLTQSP
                     ALLAAGALLLLIVTLRTERRPLRAEAPLLLLALVLFAAHLFLAGQPPRAALLPSGRIA
                     LRLLALVYLTRWAARAVLPRAARWLFGRGGIARPRPLALLVESGRMTLALLPLALAEA
                     ERQHLALRARAIRPGRGLGGRARYLAAWLLPYLGTMLRVGDSYGEALHARGYALGAPR
                     RGSASYRWAAPEAAGVLLSAAAAAWLVRAR"
     gene            5102..5899
                     /gene="truA"
                     /locus_tag="VFM00_07725"
     CDS             5102..5899
                     /gene="truA"
                     /locus_tag="VFM00_07725"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7498175.1"
                     /translation="MLDERKEGEETFRIGVEYDGTAFSGWQRQPRARTVQGELEKAVE
                     RIVGAKVTVNGAGRTDAGCHAAGQVASFTAVTRLAPERIRMAMNAHLPPDVRVVESAF
                     ASRGFHARFRAMRRSYRYLIVPEPAAIRRRLAWARRMRAGAAELNEAVAPLLGEHDFT
                     TFSRQGGDETSPVCRVTRAVWTGAKGALRFDIEADRFLYTMVRRIVSTAIAAAEQGGG
                     PTAVRTALESKRRRLGAPPAPAHGLYLMRVRYPGLGWLPKERMHVVA"
     gene            5886..7154
                     /locus_tag="VFM00_07730"
     CDS             5886..7154
                     /locus_tag="VFM00_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7498176.1"
                     /translation="MSSLDAAPRAAKARGRWPISTLGAGVLFGALLLAALVLATQRPK
                     DRATETDGKPLYGGPGNDVNLAAAAGADSVGAAPASLDTSRRTAIVAAAEKVGPSVVT
                     LSVVQTQVVQTSPIPFGDDFFEPFFRDMIPQYRYREQIPSMGSGFIISSNGYVLTNEH
                     VVRNAEKITVILPDGRRFEGKVLGSHPQYDLAVVKIEGKDLPTAKLGTSSDLMVGEWA
                     LAIGNPFGFLLNDTQPTVTAGVVSATHRDIKSQASSAGIYKNMIQTDAAINPGNSGGP
                     LVNAKGEVVGINTFIFTKSGGSEGIGFAIPIDAAKKVVDELLHYGKVRNVWIGVRTWE
                     LTPYVAERLGTKDRNGLYVSVIEKGSPADKAGIKVGDIIRKVNGTPIRDVDEAYRALF
                     GANVGDTISLTVERDGKLLTMSLALTELPE"
     gene            7151..8464
                     /gene="purB"
                     /locus_tag="VFM00_07735"
     CDS             7151..8464
                     /gene="purB"
                     /locus_tag="VFM00_07735"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET7498177.1"
                     /translation="MIARYSRPAMAGLFDDRARYATWLDVELAVTRALERRGEAPAGA
                     AARIAAAARIDPARIDELEATLRHDVIAFLTQVGETAGADARYLHLGMTSSDLVDTAL
                     ALTLVRATDLLLPEVDGLREETRRLALRHKDTVMVGRTHGIHAEPITFGLKALVWYEE
                     LGRARERLQRARATIAVGKLSGAVGTLAHLTPDLEEEVLASLGLSPEPAATQVVQRDR
                     HAELLQMLALLGASLEKIAMEIRHLQRTEVLEAEEPFREGQKGSSAMPHKRNPVNCER
                     VCGLARILRANAHAATEDVALWHERDISHSSVERVILPDSFLLADFMTAQMREILAGL
                     HVYPDRMRSNLEATRGLVYSQRALLALTGAGLSREDAYALVQRHAMAAWKEPPDLLAR
                     LQADPAATKVLDRDALARCFDPSYFLRHVNQIFDRVLGATVAAAR"
     gene            8448..10814
                     /gene="purL"
                     /locus_tag="VFM00_07740"
     CDS             8448..10814
                     /gene="purL"
                     /locus_tag="VFM00_07740"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET7498178.1"
                     /translation="MQRLAEAPTIPLMAADPARRKALLEAEGLRLTADEAARMLELLG
                     RDPTRVEATIFDTMWSEHCSYKSSRRVLQAHLPTKSPDVILGPGEDAGVVRFGTWKGV
                     EYALVFAHESHNHPSQVVPVEGAATGIGGIVRDVNCMGAEVIGVMDALRFGDPEGPRA
                     SSVREIVRGVVDGIWQYANALGVPNLGGDVFFSPRFDENCLVNVVALGLVRADRIVRS
                     RVPETASREPYVLVLVGKPTDETGFGGASFASAILEENTEGQRGHVQVPDPFLKRVLF
                     EANRAALDWLNAQRAAFGFKDLGAGGIACASSELAAAGGFGMDVDLDQIPTAPGDYPA
                     EVIACSETQERFALVVPERLAEGLLKIYNEDYALPLVYHGARAAVAGRVRADQRYRIL
                     RGGQPVAEAPVEVITAGIEHDRPRRPRPAEPPETPLPPVKDHGAFFRSMVTSPNVASR
                     APILRYYDTEVQARVVIRAGEADASVLAPVPGAPLGCALTVDGNPWYVAADPYWGTAH
                     VVLEALRNLACVGARPIALTDCLNFGNPEDPEVFDEFVRSVRGLGDAARELAPEGPSG
                     PPVAVVSGNVSFYNESATGRSIEPSPVVAALGALEDYSVAVSSALRAHPGVLVLSGPR
                     GAATGASQLRHLLTGETGGTLPPLDFDLERRRLNAVREAVRRGLVRSCHDISEGGLAV
                     AALEMALGGFASGGFGLQIPISALGDTPAEVRLYAEAPGFLMEASNDALGALLDLFAK
                     EGVDATMIGRTLPEPKLRFLDGGTTLIEADLAELRELYGGALRPYVEA"
     gene            10817..11659
                     /gene="purQ"
                     /locus_tag="VFM00_07745"
     CDS             10817..11659
                     /gene="purQ"
                     /locus_tag="VFM00_07745"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HET7498179.1"
                     /translation="MARAAVIQIPGVNCEYESARALERVGIEASILRFNEPASVLSEF
                     DAYLLPGGFAFQDRIRAGAVAAKLPAVERIAAESERGKIVLGICNGAQVLVEAGLVPG
                     IRPGRVELALAPNRAPRRQGYLCRWIWVRAEEGPGRDAFTAALEPGAVVPLPMAHGEG
                     RFETADPEVRRAIESEGLALLRYVAPDGSPARGWPDDPNGAMLHAAGITNRGGNVLAF
                     MPHPERANALKHVPLDLPGPWGQARRQAVGSVEALEGDGPGAFLFRSLAARLGAALPA
                     GRAG"
     gene            11656..12276
                     /locus_tag="VFM00_07750"
     CDS             11656..12276
                     /locus_tag="VFM00_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498180.1"
                     /translation="MSAVLLVRLKIPDLTALTALDAGRALLPAGHELARLVREEVVLF
                     EPAGKARTPGSAAARELDGALLNAVETSNFFVNPNKERYRLIAASERGEAWAPPEGSW
                     GILTRPRGDTRDAGLLERLQREHPLAGLSAIRRGRVWWLWTRGSDGSLGREGALEALG
                     PVEHAGRGLLVNPHSGSWLVLPGPVPWARIETFLAAPEPAAAATTT"
     gene            12288..13718
                     /gene="purF"
                     /locus_tag="VFM00_07755"
     CDS             12288..13718
                     /gene="purF"
                     /locus_tag="VFM00_07755"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET7498181.1"
                     /translation="MDEAQDDRLREECGVFGIWGAENAARLAYAGLYALQHRGQESAG
                     IVASDGQEFHAHRGLGLVSDVFAGGVLDRLTGHVAIGHNRYSTTGRNHPQNAQPLVVN
                     YREGDLAIGHNGNLVNALEMRREMEEQGSIFQTTSDTEVILHLIARSKATELERMIPE
                     ALARCQGAYSLVILAGETLVGVRDPRGFRPLCLGRRGTAHVLASETCALDIVGADFVR
                     EIDPGEMVVIDRNGVRSFRILTPEPQRACVFELIYFSRPDSVVFGASVDSVRRKLGRR
                     LAEEHPADADIVISVPDSSNSAALGFAEASGLPFELGLIRNHYVGRTFIAPEQITRSF
                     GVNMKFNAVRRILDGKRVVVVDDSIVRGTTSRSLVMMIRAAGAKEVHFRVSSPPIGWS
                     CYYGIDTPNRRELIASSHTIEEIRKYLHVDSLGYLSMDGLRSCVEKPDSHCYACFNGD
                     YSVRFGEEMDKLALERPTVKNGGAKA"
BASE COUNT         1915 a         5062 c         5081 g         2099 t
ORIGIN      
        1 gatcgccccg ggggacgtcg tgctcggcct gcccagcgcg ggcctccata cgaacggcta
       61 ctcgctcgcg cggaagatcc ttctcgagaa gctgcgcctg cgcgtcggcg atcgctttcc
      121 gggcgcggcc gagacggtgg gagaggtgct gctcgcgccg cacctctact acggtcccga
      181 cgtccggatc gcccgcgaag cgggaaccat ccgcggcatg gcgcacatca ccggcggtgg
      241 gatccccggc aacctggtcc ggatcctccc cgagggcgtg cgcgccgaga tccggatggg
      301 gtcctggccg gtgccgcacg tcttccggac cctcgccgag gcggggggcg tgcccgacgc
      361 ggaggcctac gaggtgtgga acatgggact cggcctcctg ctcgtgacgc ccgccggcga
      421 cgcggaccgg atcgaagcgg cgctcgccgg tgccggccgc gcctgcttcc gcgccggagc
      481 catcgccgcc ggcccgcgtg aggtgcgtct gattccgtag gcttcggatc cgaacgggag
      541 tggggagggg agcgtggtgg aacgaatcga agcccaggcg gaccgtcccg tgccggtccc
      601 ggcgctcgct cccggcggtc cgggaccgta cgccggagcc ctcgggctgg tcgggctgag
      661 cgccccgctc cgcgccctct gcgagcggat cgccgcgcac gcgcggagca gcgcgaccgt
      721 gctcatccgc ggcgagaccg gctcgggcaa ggagctggtg gcgcgagcgc tccaccgctt
      781 gagcccgcgc gccgggcgcg ccttcctgcc ccacaacttc gcctccattc ccgattcgct
      841 cgtggagagc gagctcttcg ggcacgcccg cggggcgttc accggcgcgc attccgaccg
      901 tcccgggctc ttcgagctgg cggacggcgg caccctcttc ctcgacgaga tcggcgacgc
      961 gagcccttcg gtccagagcc ggctgctccg cgtcctccag gagggggaag tgcggcgggt
     1021 gggggatggg cgggcgcggc gcgtggacgt ccgtatcgtc gccgccacgc accgcgatct
     1081 cgcgggcgaa gcgcgcgccg gccgctttcg cgccgacctc tactaccggc tgcacgtgct
     1141 cgcgctggac gtaccgccgc tccgcgcccg tcgcgcggac gtgccggtcc tggcggcgca
     1201 catcttggaa tcgctcgcgc gccgcgacgc gctcgccgta cgcggcgtcc atccggccgc
     1261 gctccagctc ctgctggccc acgactggcc gggcaacgtg cgcgagctgg aggcggtgct
     1321 gacccgcgcc gcccacagcg ccggcgccgg cggcgtgatc gagccggagg cgctgggccc
     1381 cgaggtcctg ggcgagcgcc cgttcctcgt gcgcgagcgg cacgccacgc tgcgcggccg
     1441 cacgctcgcc tacgaggcgg agctgatccg gtgcgcgctc gacgtgcacc gggggaaccg
     1501 ctcgctggcc gcgcgctcgc tcgggctctc gcggcaggga ctgtggaaga agatgcggcg
     1561 cctcggggtg cgcgcggaaa gggacccgat cgaagcggcc gaagcggccg attgaccttg
     1621 atttcgccgg tcgcatttct atactcgcgc gacatcgtcg tttcccattc gtccgccgcc
     1681 cgctcgcacc gcgcggcgcg cgctcctcgg gaggaactcc ttggccaagc gtcccctgcg
     1741 gatcggcgtg atcggggccg gcctcgtcac gcagatcaat catctccccg cgctcaagaa
     1801 gcgccgcgac gccgaagtgg tcgcggtgtg cgacgacgac ggggaaaagg cgcgcatggt
     1861 cgcgcggcac ttcggcatcg cccgcacgct caccgactac gagaccctct tgcgcagcga
     1921 cgaggtcgtg gcggtgatcg tcgcgacgcc caaccatctg cacgccccga tggtgctggc
     1981 cgcgatgggt tacggcaagc acgtgctctg cgagaagcct ccggcaagga acgccgccga
     2041 ggccgagcag atggcggagg ccgcgcggcg ctccggctgc acgctgatgt acgccatgaa
     2101 caaccggttc cggaccgacg tgcagctctt gcgcggctac atggagcggc gcgaattcgg
     2161 taccatcttc tacgccaaga cggggtggct gcggcggcgc acggcgcagc gcggtcccgc
     2221 ctggtaccag aacaagcgca gctccggggg cggcgtcctc atggacctcg gcgtccagat
     2281 gctcgacctc tccctctggc tcctgggcaa cccccaagtg gcgtcggtga cggcgaccaa
     2341 atatgtgacc gatccgcgcg aggacgtcga ggacaccgtc gcggcgttcc tggtgctgga
     2401 cagcggggcg gcgctgacgc tggaggtgag ctgggcgctg ctgctcgaaa agaacttccc
     2461 gtatctgaac ctgtttggca cgcacggggc ggccctgctg aaccccttcc gggtccacaa
     2521 ggagctgggg gggaacctcc tcgacgtgac gccgcccctg gagtcgaaga acgtctacaa
     2581 gcagtcgtac gaagcggagc tcgaccattt catccgatgc atcacgcagg gcgagcggcc
     2641 cgtgggcacg cccgaagagg ggctccagct catgagggtc atcgacgcca tctaccagtc
     2701 cgctgagagc cgccgcgagg tccgcctcca ttagatcggt gcgcgcgccc ctggccgcgg
     2761 ccgcgtcggg catcctcgcc agcctcgcct tcccctcggc atcgctctgg ccgctgatct
     2821 tcgtcgcgct cgtgccgctg ctcctggttc tggcctccgg ctcgcccggg ccgcgcgatc
     2881 ccgagccggc ctcccgcggc gtgcgctggg cgccgtggct catggggacg gtgttctacg
     2941 ccctcacgtt ctggtggatc gtccgcctgc cggtcgcggc gatgacccac ccctggctca
     3001 tctatccggg tctgctcgcg ctcgggctct atcttggcct ctacgtcgcg ctcttcggct
     3061 ggctcgtgcg cttcgtgcgg cgccggctcg gctggcccgt gctggcgctc gcgcccttcg
     3121 cgtggggcgt ggcggaatgg ctcaagtcct cgggagagct ggggtgcccc tgggggaatc
     3181 tgggcaccgc gctcgccgag catccggcct ggatccaggg cgcgtcgctc gccggcgcca
     3241 cggggctctc cttctgggtc gtgtcggtga acgcgctggt cgccgccatc cttctcgcgc
     3301 gccgctggtc ctcgcggatc gggctcgcga tgatcctggc cgccgccgtc ggcgtccccg
     3361 tggtctgggg aacggcgcgg ctcaaggctc ccgcccccgc gccgctggtg cgcgtcgcca
     3421 tcgtgcagcc gaacgtgggg agccacgaga agtgggtccc ggcgcttcaa gattcggtca
     3481 ccgcgcgcgt ggagcggctc acccgggaag cggggatggc cgacccgcgc ccgacggtca
     3541 tcctctggcc cgagacggcg cttccgtact acgtgcgggt ggagccgctc aagctgcagc
     3601 ggctgatcgc gctcgcgaag gaggtgaacc gcccgatcct ggccggctat cccgacgcga
     3661 agctgaacgc gcagggagac gtgctcacct acaacgcggc ggggctcgtc ctgcccgcgg
     3721 gggcgatcgt cggccagtac gaaaaaatcc acctcgtccc cttcggagag cgcatcccgt
     3781 tccagagcct ctttccgttc ctgggctcga tcgacctggg ccaggcggag tggactccgg
     3841 gaacgcgacc gtacgtcttc cgccgcgcgg gaccggcgtt cggcgtcctc atctgcttcg
     3901 agtcgatctt cccggatcac acgcgccgct acgccctcga gggggcgcag tacctggtca
     3961 acatcaccaa cgacgagtgg ttcgggcgga cggcgggacc gatccagcac gccgacctgg
     4021 cgatccttcg ctcggtcgag ctgggcatgg gcatcgcgcg cgcggcgaac accggcgtct
     4081 cgatgctcat cgatccctat gggcgggtcg aggcgcgcac gccgctcttc gccgaggcgg
     4141 tcctggtcgg cacggtgaag accgggatcg ggcccacccc gttcgcgcgc tggggcgact
     4201 ggatctcgat gctctccctc gcggtgacgc tgatcctcgt cgcggtgggg tggttccggg
     4261 gcgttgacag ccgggccaag cttcgctagg gtggggcttc aggtggagtg cgtcagcgac
     4321 ttcacctccg acggcacgcc aagtccagag gaacggcaag cgacagtgcc cggctcaccc
     4381 gagtccgacg ccacgaaacc gaaatcgacg tcccgcgcgc ggcgcccgct gcatcccggc
     4441 gcgtggctct tcgtctgcgc ggccctgggc gtgggcttct cgctcacgca gtcgcccgcc
     4501 ctgctcgccg cgggagcgct gctccttctg atcgtcacgc tccgcaccga gcgccgtccg
     4561 ctccgcgccg aggcacccct tcttctcctc gcgctcgtcc tgttcgcggc acacctcttc
     4621 ctggccgggc agccgccgcg cgcggcgctt ctcccctcgg gccggatcgc cctgcgcctc
     4681 ctggccctgg tctacctgac gcgatgggcg gcccgcgccg tcctgccgcg ggcggcccgc
     4741 tggctcttcg gccgcggcgg catcgcgcgg ccgcgcccgc tggcgctcct cgtcgagagc
     4801 ggacggatga cgctggcgct cctgccgctc gcgctggccg aggccgagcg gcagcacttg
     4861 gcgctccggg cgcgcgcgat ccgtccggga cgcggcctcg gcggccgggc gcgctacctc
     4921 gccgcctggc tccttccata cctgggcacg atgcttcgcg tcggcgacag ctatggcgaa
     4981 gcgctgcacg cccgcggcta cgcactcggg gctccacgcc ggggctccgc ttcgtaccgg
     5041 tgggccgcac ccgaagcggc gggtgtcctc ctctccgcgg cggcggccgc atggctcgtc
     5101 cgtgctcgat gaacgaaagg agggggagga aaccttccgg atcggggtgg aatacgacgg
     5161 gaccgcgttc agcggctggc agcggcagcc ccgggcgcgc acggtgcagg gggagctgga
     5221 aaaggccgtg gagcggatcg tcggcgccaa ggtcacggtg aacggagcgg ggaggacgga
     5281 cgccgggtgc cacgccgcgg gacaggtggc gtcctttacg gcggtcacgc ggctcgcgcc
     5341 ggagcggatc cgcatggcga tgaacgcgca cctgccgccc gacgtccggg tggtcgagtc
     5401 ggcgttcgcg tcgcgcggct tccacgcccg cttccgcgcg atgcgccgga gctaccgcta
     5461 cctcatcgtt ccggagccgg cggccatccg gaggcggctg gcctgggcgc gccggatgcg
     5521 cgccggggcc gcggagctga acgaggcggt cgcgccgctc ctcggcgagc acgacttcac
     5581 gaccttctcg cggcaggggg gcgatgagac gtcgccggtc tgccgcgtca cccgcgccgt
     5641 gtggacgggc gccaagggcg ccctccgctt cgacatcgag gcggaccggt tcctgtacac
     5701 tatggtgcgc cgcatcgtat cgaccgcgat cgccgccgcc gagcaggggg gcgggccgac
     5761 cgccgtccgg acggcgctcg aatcgaagcg ccggcgcctg ggagcgccgc cggccccggc
     5821 gcacggcctc tatctcatgc gggtccgcta tcccgggctc ggatggcttc cgaaggagcg
     5881 aatgcatgtc gtcgcttgac gcggcgccgc gggccgcgaa agcccggggc cgctggccga
     5941 tttccacgct gggcgcgggc gtgctcttcg gcgccctcct cctcgccgcg ctcgtgctcg
     6001 cgacgcagcg gcccaaggac cgcgcgaccg agacggacgg caagccgctc tacggcggcc
     6061 ctgggaacga cgtgaacctg gccgcggcgg cgggcgccga ttcggtcggc gcggcgccgg
     6121 cttctctcga tacctcccgg cgcacggcga tcgtcgcggc cgccgagaag gtgggcccct
     6181 ccgtggtgac gctgagcgtg gtccagaccc aggtggtcca gacctcgccc atccccttcg
     6241 gcgacgattt cttcgagccc ttcttccggg acatgatccc gcagtaccgc taccgcgagc
     6301 agatcccgag catgggctcg ggcttcatca tctcgtcgaa cggctacgtg ctcacgaacg
     6361 agcacgtggt gcgcaacgcc gagaagatca ccgtgatcct ccccgacgga cgccggttcg
     6421 aggggaaggt gctcggcagc catccgcagt acgacctggc cgtcgtgaag atcgagggca
     6481 aggacctgcc cacggcgaag ctcgggacct cgagcgacct catggtgggc gagtgggcgc
     6541 tggcgatcgg gaaccccttc gggttcctgc tgaacgacac ccagccgacc gtgaccgcgg
     6601 gcgtcgtgag cgcgacccac cgcgacatca agtcgcaggc gtccagcgcg gggatctaca
     6661 agaacatgat ccagaccgac gcggccatca acccgggcaa ctccggcggc cccctcgtga
     6721 acgcgaaggg cgaggtcgtc ggcatcaaca ccttcatctt cacgaagagc ggcggctcgg
     6781 aggggatcgg cttcgccatc ccgatcgacg ccgccaagaa ggtcgtggac gagctgctcc
     6841 attacggcaa ggtgcggaac gtctggatcg gcgttcgcac ctgggagctc accccctacg
     6901 tcgccgagcg gctcggaacc aaggaccgga acgggctcta cgtctcggtg atcgagaagg
     6961 gaagcccggc cgacaaggcc ggcatcaagg tgggcgacat cattcgcaag gtgaacggga
     7021 cgccgatccg ggacgtggac gaggcctacc gcgcgctctt cggcgcgaac gtcggcgaca
     7081 cgatctccct taccgtcgag cgcgacggca agctgctcac gatgagcctc gcgctcaccg
     7141 agctgcccga gtgatcgcgc gctacagccg tcccgcgatg gcgggtctct tcgacgaccg
     7201 ggcgcgctac gcgacctggc tcgacgtcga gctggccgtg acgcgcgcgc tggagcggcg
     7261 cggggaagcg cccgcgggcg cggccgcgcg gatcgcggcc gcggcgcgca tcgatcccgc
     7321 gcggatcgac gagttggaag ccacgcttcg ccacgacgtg atcgccttcc tgacgcaggt
     7381 gggggagacc gccggcgccg acgcgcgcta cctgcacctg ggcatgacct cctccgatct
     7441 cgtggacacc gcgctcgcgc tcacgctggt gcgcgcgacc gacctgctcc tgcccgaggt
     7501 ggatggcctg cgcgaggaga cgcgccgcct ggcgctccgc cacaaggaca cggtgatggt
     7561 cgggcgaacg cacggcatcc acgccgagcc gatcaccttc gggctcaagg cgctcgtgtg
     7621 gtacgaggag ttggggcgcg cgcgggaacg gctccagcgc gcgcgcgcga ccatcgcggt
     7681 ggggaagctc tcgggcgcgg tcggcacgct ggcccacctg acgccggatc tcgaagagga
     7741 ggtgctcgcc tcgctgggct tgagccccga gccggccgcc acgcaggtgg tgcagcgcga
     7801 ccgccacgcg gagctcctcc agatgctggc cctcctcggc gcctcgctcg agaagatcgc
     7861 gatggagatc cgccacctgc agcgcaccga ggtgctcgag gcggaggagc cgttccgcga
     7921 ggggcagaag ggctcttcgg cgatgcccca caagcggaat ccggtgaact gcgagcgggt
     7981 atgcggcctc gcgcgcattc tgcgcgcgaa cgcgcacgcg gcgaccgagg acgtggcgct
     8041 ctggcacgag cgggacatca gccattccag cgtggagcgc gtgatcctcc ccgactcctt
     8101 cctgctcgcc gacttcatga cggcgcagat gcgggagatc ctggcggggc tgcacgtcta
     8161 tcccgaccgc atgcgctcga acctggaagc gacgcgcggg ctggtctact cccagcgcgc
     8221 gctgctcgcg ctgaccgggg cggggctctc gcgcgaggac gcctacgccc tggtgcagcg
     8281 ccacgcgatg gcggcgtgga aggagccgcc cgatctcctg gcgcggctcc aggccgatcc
     8341 cgcagcgacc aaggtgctgg accgggatgc gctggcccgc tgctttgatc cgtcctactt
     8401 cctgcggcac gtgaaccaaa tcttcgatcg ggttctggga gctaccgttg cagcggctcg
     8461 ctgaggcacc gaccatcccg ctcatggcgg cggatccagc gcggcggaag gcgctgctcg
     8521 aagccgaggg gctccggctc acggcggacg aagcggcgcg catgctcgag ctgttgggcc
     8581 gcgaccccac ccgcgtcgag gcgaccatct tcgacacgat gtggagcgag cactgctcct
     8641 acaagagcag ccgccgcgtg ctccaggcgc accttcccac gaagagtccc gatgtgatcc
     8701 tgggcccggg ggaagacgcc ggcgtcgtgc gcttcggcac ttggaagggc gtcgaatacg
     8761 ccctcgtctt cgcgcacgag agccacaacc atccctccca ggtcgtgccg gtcgaagggg
     8821 cggccaccgg catcggcggc atcgtgcgcg acgtgaactg catgggggcc gaggtgatcg
     8881 gcgtcatgga cgccctccgc ttcggcgatc ccgaagggcc gcgcgcctcc tcggtgcgcg
     8941 agatcgtgcg cggcgtggtg gacgggatct ggcagtacgc caacgcgctg ggcgtcccca
     9001 atctcggtgg ggacgtcttc ttctcgccgc gattcgacga gaactgcctc gtgaacgtcg
     9061 tcgcgctggg actggtgcgc gccgaccgca tcgtgcggag ccgcgttccc gaaacggcct
     9121 cgcgcgagcc ctacgtcctc gtgctggtgg ggaagccgac cgacgagacc gggttcggag
     9181 gcgcctcctt cgcgtcggcc atcttggaag agaacaccga agggcagcgc ggccacgtgc
     9241 aggtgcccga ccccttcctg aagcgggtgc tcttcgaagc caaccgggcc gcgctcgatt
     9301 ggctgaacgc gcagcgggcg gcgttcggct tcaaggacct cggcgccggc gggatcgcgt
     9361 gcgcctcgag cgagctggcc gcggccggcg ggttcgggat ggacgtggac ctggaccaga
     9421 tccccaccgc gcccggcgac tatccggccg aggtgatcgc ctgctccgag acgcaggagc
     9481 gcttcgcgct cgtggtgccg gagcggctgg ccgaggggct cctcaagatc tacaacgagg
     9541 actacgccct cccgctcgtc tatcacggcg cgcgcgccgc ggtggcgggt cgggtgcgcg
     9601 cggaccagcg ctaccggatc ctccgcggcg gccagccggt ggccgaggcg ccggtcgagg
     9661 tgatcaccgc ggggatcgag cacgaccgcc cgcggcggcc tcgtcccgcc gagccgcccg
     9721 agacgccgct gccgccggtg aaggaccacg gcgccttctt ccgctccatg gtcacgtcgc
     9781 cgaacgtcgc ctcgcgcgcg ccgatcctcc gctactacga caccgaggtg caggcgcgcg
     9841 tcgtgatccg cgccggagag gcggacgcgt cggtgctcgc gccggttccc ggcgctccgc
     9901 tcggctgcgc cctcaccgtg gatggaaacc cgtggtacgt cgcggccgat ccctactggg
     9961 ggacggcgca cgtcgtgctc gaggcgctcc ggaatctcgc ctgcgtcggg gcccgcccga
    10021 tcgcgctcac cgactgcctc aacttcggaa accccgaaga ccccgaggtc ttcgacgagt
    10081 tcgtccggag tgtccgcggt ttgggcgacg ccgcgcgcga gctcgcgccc gaggggccct
    10141 cggggccgcc ggtcgccgtg gtctcgggca acgtctcctt ctacaacgag tcggcgaccg
    10201 ggcgctccat cgagccctcg ccggtcgtgg ccgcgctcgg cgccctggag gactacagcg
    10261 tcgccgtctc gtcggcgctc cgcgcccatc cgggcgtgct cgtgctgagc gggccgcgcg
    10321 gcgcggccac cggcgcctcg cagctccgcc accttctgac gggggagacc ggcggcacgc
    10381 ttccgccgct cgatttcgac ctggagcggc ggcggttgaa cgccgtgcgc gaggcggtgc
    10441 ggcgcggcct ggtccgctcc tgccacgaca tctccgaagg cggcctggcg gtcgccgcgc
    10501 tggagatggc gctcggcggc ttcgcctcgg ggggcttcgg actccagatc ccgatctccg
    10561 cgctcggcga cacgccggcc gaggtcaggc tctatgccga agcgcccggg ttcctgatgg
    10621 aagcgtcgaa cgacgcgctg ggcgcgctgc tcgacctctt cgccaaggaa ggggtggacg
    10681 cgacgatgat cggccgcacg cttcccgagc cgaagctccg cttcctggat gggggcacga
    10741 ccctgatcga ggcggacctg gccgagcttc gcgagctgta cggcggcgca ctccgtccct
    10801 acgtggaggc gtgacgatgg cgcgcgcggc ggtgattcag attccgggcg tcaactgcga
    10861 gtacgagagc gcgcgggcgc tggagcgcgt gggcatcgaa gcgtcgatcc tccgcttcaa
    10921 cgagcccgct tcggtcctct cggagttcga cgcctatctc ctgccgggcg gcttcgcgtt
    10981 ccaggaccgg atccgcgcgg gggcggtcgc ggccaagctg cccgccgtcg agcggatcgc
    11041 ggccgagtcg gagcggggga agatcgtgct gggaatctgc aacggagcgc aggtgctggt
    11101 cgaggcgggg ctcgtgcccg ggatccgccc cggccgcgtg gagctcgcgc tcgcgcccaa
    11161 ccgggcgccg cggcgccagg ggtatctctg ccgctggatc tgggtgcgtg ccgaagaggg
    11221 cccgggccgc gacgccttca ccgcggcgct cgaacccggc gcggtggtgc cgcttccgat
    11281 ggcgcacgga gagggacgct tcgagaccgc cgatcccgag gtgcgccgcg cgatcgagtc
    11341 ggaggggttg gcgctcctgc gctacgtcgc gccggacggt tcgccggcgc gcggctggcc
    11401 ggacgacccc aacggggcca tgctgcatgc ggccggcatc acgaaccggg gcggaaacgt
    11461 cctcgcgttc atgccccacc ccgagcgcgc gaacgccctg aagcacgtcc cgctggacct
    11521 gcccggcccc tggggccagg cgcggcgcca ggccgtgggg agcgtggagg cgctggaggg
    11581 agatgggccc ggggcgttcc tcttccggtc gctggccgcc cgattgggcg cggcactgcc
    11641 ggcggggaga gcggggtgag cgcggtcctc ctggtacggc tcaagatccc cgacctcacc
    11701 gcgttgaccg cgctcgacgc cggccgggcg ctcctgcccg cgggccatga gctcgcgcgg
    11761 ctggtgcggg aagaggtggt cctgttcgag ccggcgggga aggccaggac cccggggtcg
    11821 gccgcggccc gggagctgga cggggccctc ctcaatgcgg tggagaccag caacttcttc
    11881 gtgaatccaa acaaggagcg ctaccgcctg atcgccgcct ccgagcgggg ggaggcctgg
    11941 gcgccgcccg agggaagctg gggcatcctg acgcgcccgc gcggagatac gcgggacgcc
    12001 gggcttctcg agcgcctgca gcgggagcac cctttggcgg ggctttcggc catccggcgg
    12061 ggacgggtct ggtggctctg gacccgaggc tccgacggaa gtctggggcg ggagggggcg
    12121 ctcgaggcgc tggggccggt ggagcacgcc ggccgggggc tcctggtgaa cccccattcg
    12181 gggtcgtggc tcgttcttcc gggtcccgtg ccctgggcgc gcatcgaaac gtttctcgcg
    12241 gcgccggagc cggcggccgc ggccaccacc acctgagagg agccatcgtg gacgaagcgc
    12301 aggacgaccg gctgcgggag gaatgcggcg tgttcggcat ctggggcgcg gagaacgcgg
    12361 cgcgcctcgc ctacgccgga ctctacgcgc tccagcatcg cgggcaggag agcgccggga
    12421 tcgtggccag cgacgggcag gagttccacg cccaccgcgg cctgggcctc gtctccgacg
    12481 tctttgccgg cggcgtcctc gaccgcctga cgggccacgt cgcgatcgga cacaaccgct
    12541 actcgaccac gggccgcaac cacccccaga acgcgcagcc gctcgtcgtg aactaccgcg
    12601 agggggacct cgcgatcggg cataacggaa atctcgtgaa cgcgctcgag atgcggcgcg
    12661 agatggagga gcagggctcc atcttccaga ccaccagcga caccgaggtg atcctccacc
    12721 tgatcgcgcg ctccaaggcg acggagctgg agcggatgat tcccgaagcg ctggcgcgct
    12781 gccagggcgc ctactcgctc gtgatcctcg cgggggagac cctggtcggg gtgcgcgacc
    12841 cgcgcggctt ccgcccgctc tgcctgggac ggcgcggcac ggcgcacgtg ctcgcgagcg
    12901 agacctgcgc gctcgacatc gtgggcgccg acttcgtccg cgagatcgac ccgggggaga
    12961 tggtggtcat cgaccggaac ggggtgcgct cgttccggat cctcacgccc gagccgcagc
    13021 gcgcctgcgt cttcgagctg atctacttct cccgccccga cagcgtcgtc ttcggcgcga
    13081 gcgtggactc ggtgcggcgg aagctgggac ggcgcctggc cgaagagcat ccggccgacg
    13141 ccgacatcgt catctcggtg cccgactcga gcaactccgc cgctctggga ttcgccgagg
    13201 cgtcggggct tcccttcgag ctgggcctga tccgcaacca ctacgtcggg cgcacgttca
    13261 tcgcccccga gcagatcacc cgctccttcg gcgtgaacat gaagttcaac gcggtgcgcc
    13321 gcatcctgga cggcaagcgc gtcgtggtcg tggacgactc catcgtgcgg ggcacgacca
    13381 gccggagcct cgtgatgatg atccgcgccg ccggcgcgaa ggaagtccac ttccgggtct
    13441 ccagcccgcc gatcggctgg tcctgctact acggcatcga cacgcccaac cgccgcgagc
    13501 tgatcgcttc ctcgcacacg atcgaggaga tccggaaata cctgcacgtc gactccctgg
    13561 gctatctctc catggatggg ctgcgctcct gcgtggagaa gccggactcc cattgctatg
    13621 cgtgcttcaa tggcgactac tcggtgcggt ttggcgagga gatggataag ctggcgctcg
    13681 agcggcccac tgtgaagaac ggcggggcca aagcgtagag cactcgacgg ccgtgcccac
    13741 aggcggccgg gcgctacgcg ttggcgcgag gggaggcagc ccttcgggcg cgccgccctc
    13801 cggcccgact ctcatcgctc gtttggtagc ctcctcggcg ggcctccggg agggcgccct
    13861 ccgcggctgt ctcccctcgc gtccaaggat tgcgtctcga cctgccggcg cgtgctcgtc
    13921 atacgacttc aatcgaggcg agttccgagg cgggaccggc gaccaaagtt ttaccgccgc
    13981 cagcggcggt gaatcgatgg acaagtgtct tcgtggagtg acgcgacaga acgtcgcgct
    14041 ccctttctcg gtcgatctca tcaagtgcct tccgtcggtg aagcgcaccc agcttctcgg
    14101 ccctttggct actggcaagc tctcgaccgt cccctcacca agatcgcctg cttttgg
//
LOCUS       DASSLZ010000200         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_832594, whole genome shotgun sequence.
ACCESSION   DASSLZ010000200 DASSLZ010000000
VERSION     DASSLZ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832594"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFM00_07760"
     CDS             <1..329
                     /locus_tag="VFM00_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7498182.1"
                     /translation="LEGVNFITDSAELTPESKATLDRTAASLARWPEVRVEVGGHTDS
                     MADDAYNMQLSQRRAETVRAYLIRAGISARRLEAKGYGETRPIAPNDTEAGRAKNRRV
                     ELTRID"
     gene            complement(326..871)
                     /locus_tag="VFM00_07765"
     CDS             complement(326..871)
                     /locus_tag="VFM00_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HET7498183.1"
                     /translation="MAPPGPVMAIMSTASTQGRPREAIRTALGAMTADATWLALVTLG
                     FLAVLRRHPRIVGALGAAGGAMLLWMAWDGIRTLRRGAAASSARGSYRLGLMTVLASP
                     FSFGWWMASGPIVISSLGATGIAGLFLSILVYAVAISYALAWLGARVAHTATVFAWIG
                     ILMLAVFGVLFIAEGARLVRG"
     gene            complement(921..1583)
                     /locus_tag="VFM00_07770"
     CDS             complement(921..1583)
                     /locus_tag="VFM00_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498184.1"
                     /translation="MTGLRTALRRPVLAAALALALFPGCLTYEKSQTKVAFDPKTGVA
                     HVQTTYWNIASTEPGRREQWDDFEQLDSLRRSDAYFATSYLRSPAGGQVGKRRVWIEK
                     GQVHASAAVTTRDLNELAEGWSADSTGYRFTSNLEITATNGRRTDDEKPTVFWPRSAR
                     LLSVAERDPHFGEAVPFRPEIRDSLAKRLPPVLKAAHEKRVAKHRTVRRGRAGSKRRR
                     SH"
     gene            complement(1580..>2072)
                     /locus_tag="VFM00_07775"
     CDS             complement(1580..>2072)
                     /locus_tag="VFM00_07775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET7498185.1"
                     /translation="YGSLAAYASAQLDPSLTWESIEWFRKRTRLPIVLKGIVRADDAR
                     RAVDCGADGVWVSNHGGRQLDGCEAAVLALPAVADAVGSGAEVYVDGGIRRGSEVVKA
                     LALGARAVFVGRPVLWGLAVGGERGVLRVLDLLREEVALSLALAGCADLRSADRSLVV
                     SCA"
BASE COUNT          327 a          747 c          745 g          253 t
ORIGIN      
        1 tcctggaagg ggtgaacttc atcaccgaca gcgccgagct cacgcccgag tccaaggcca
       61 cgctggaccg caccgcggcc tcgctcgcgc ggtggcccga ggtgcgcgtg gaagtcggcg
      121 ggcacaccga ctccatggcc gacgacgcct acaacatgca gctctcgcag cggcgcgccg
      181 agaccgtgcg cgcctatctg atccgcgcgg gaatcagcgc gaggcggctg gaggcgaagg
      241 gttacggcga gacgcggccg atcgcgccga acgacaccga agcgggacgc gccaagaacc
      301 gccgcgtgga gctgacccgg atcgactagc cccgtacgag gcgggcgccc tcggcgatga
      361 agagcacgcc gaacaccgcc agcatgagga tgccgatcca ggcgaacacc gtggcggtgt
      421 gcgccacgcg ggcgcccagc caggcgaggg catagctgat cgcgacggcg tagaccagga
      481 tcgacaggaa gaggccggcg atgcccgtcg ccccgagcga cgagatcacg atcggaccgc
      541 tggccatcca ccagccgaag gaaaaggggc tcgcgagcac ggtcatgagc cccagacgat
      601 aggagccgcg cgccgaggag gcggcggctc ctcgtcgtag ggtccggatt ccatcccagg
      661 ccatccacaa gagcatcgct ccgccggcgg cgccgagggc gcccacgatg cgcgggtggc
      721 ggcggagcac cgccaggaag ccgagggtta ccagcgcgag ccacgtcgcg tcggcggtca
      781 tggccccgag cgccgtccgg atcgcctcgc gcggccgtcc ctgggtcgag gcggtggaca
      841 tgatcgccat caccgggccc ggcggcgcca cgagcgagag gccgagcagg aaacccgctc
      901 cgagacgggc gagcggatcg ttaatgcgac cgtctccgct tggagccggc gcggccgcgg
      961 cgcacggtcc ggtgcttcgc gacgcgcttc tcgtgcgccg ccttcagcac cggggggagc
     1021 cgcttcgcca ggctgtcccg gatctccggc cggaagggaa ccgcttcgcc gaagtgcgga
     1081 tcccgctcgg cgaccgagag caggcgcgcc gatcgcggcc agaacacggt cggcttctcg
     1141 tcgtcggtcc gccgtccatt cgtggcggtg atctccagat tggaggtgaa gcgatatccg
     1201 gtggagtccg cgctccatcc ctcggcgagc tcgttcaagt cccgcgtcgt gaccgcggcc
     1261 gacgcgtgca cctggccctt ctcgatccag acccggcgct tcccgacctg cccgccggcc
     1321 gggctccgca gatagctcgt ggcgaagtag gcgtcgctgc ggcgaaggga gtcgagctgc
     1381 tcgaagtcgt cccactgctc ccttcgcccc ggctcggtcg aggcgatgtt ccagtaggtg
     1441 gtctgcacgt gggcgacgcc ggtcttgggg tcgaacgcga ccttggtctg gctcttttcg
     1501 taggtgaggc agccggggaa gagtgccagg gcgagcgccg cggccaggac ggggcggcga
     1561 agcgcggtcc ggaggccggt catgcgcagg agacgaccag gcttcggtcc gcggagcgga
     1621 ggtcggcgca gccggcgagg gccagcgaga gcgcgacctc ctcgcgcagg agatccagca
     1681 cgcggagcac gccccgctcg ccgcccaccg cgagccccca gaggacggga cgtcccacga
     1741 acaccgcgcg cgcccccagt gccagggcct tcacgacctc gctgccgcgc cggatcccgc
     1801 cgtccacgta cacctcggcg ccgctcccca ccgcgtcggc caccgccggc agcgcgagca
     1861 ccgcggcctc gcagccgtcc agttgccgtc ccccgtggtt cgacacccag acgccgtcgg
     1921 cgccgcagtc caccgcgcgc cgcgcgtcgt cggcgcggac gatccccttg agcacgatcg
     1981 gaagccgggt ccgcttccgg aaccactcga tcgattccca ggtgagcgag ggatcgagct
     2041 gggccgaggc gtaggcggcg aggcttccgt ac
//
LOCUS       DASSLZ010000201         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1838931, whole genome shotgun sequence.
ACCESSION   DASSLZ010000201 DASSLZ010000000
VERSION     DASSLZ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1838931"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFM00_07780"
     CDS             complement(<1..569)
                     /locus_tag="VFM00_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HET7498186.1"
                     /translation="MTHAPSDLEPRVGADPRWRAVLARDARQDGRFVFGVRSTGIYCR
                     PSCPARKPRKNQVVFFPDADAAEHAGFRSCKRCRPRDTAPHPHAELVRRVCAHIEERA
                     AEERVTLEALARVARMSPHHLQRTFLSATGISPRQYADAVRLASLKEGLRRKEPVTMA
                     MTEAGYGSSSRLYERSPEALGMTPGDYRAG"
     gene            complement(646..1080)
                     /locus_tag="VFM00_07785"
     CDS             complement(646..1080)
                     /locus_tag="VFM00_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET7498187.1"
                     /translation="MSLVVTDEDALAAIVRGAKRVAVIGMVDEQRSDRPAYTIPEMCR
                     ETGMEVVPVNPRIESSQGLKAYPDLASVPGTFDLVNVFRRSEDVPPHADEVLALPPER
                     RPKVFWMQSGIRNDAAAAKLTAAGIQVVQDRCLGVYAARYRH"
     gene            complement(1207..>2112)
                     /locus_tag="VFM00_07790"
     CDS             complement(1207..>2112)
                     /locus_tag="VFM00_07790"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HET7498188.1"
                     /translation="WLRRAGAPSGGANHRISARPLGAEVHSAREEKPASTVLPAGGAV
                     VPMAWAGHLASVEVTLNGQGPFRFAIDTGAAGCLRIDEAVARKLGLPVVGEARVGDPS
                     GRNMKTAEIVAVDSLAAGDARFAGLTATTGDFAGRLPGEKVDGILGFGLFSDCLLTLD
                     FPALRMTLAQGSLPLDGTGIVPYRDERGIPSVTMRVAGVDVDTDVDAGAMGGFSLPES
                     FAAKLPLAEPPRVVGHARTVSNEFDIKAAGLEGDVAVGPTTFAKPMVEFQPVFDVGNI
                     GARVLRDFAVTFDPKNHRMRLSRSA"
BASE COUNT          317 a          738 c          739 g          318 t
ORIGIN      
        1 ccggcgcggt agtcgccggg ggtcatcccc agcgcctcgg gagaacgctc gtagaggcgg
       61 ctgctcgatc cgtatcccgc ttccgtcatg gccatcgtga ccggctcctt tcgacgcagc
      121 ccttccttga gcgacgccag ccgcaccgcg tccgcgtact ggcgcggcga gatgcccgtg
      181 gcgctcagga aggtgcgctg cagatgatgg ggcgacatgc gcgcgacccg cgcgagcgcc
      241 tccagggtga cgcgctcctc ggccgcgcgc tcctcgatgt gggcgcagac gcgacggacc
      301 agctccgcgt ggggatgggg ggcggtgtcc cggggacggc atcgcttgca ggagcggaat
      361 cccgcgtgtt ccgccgcgtc agcgtcgggg aagaacacga cctggttctt ccgcggcttt
      421 cgcgccgggc aggaggggcg gcaatagatg ccggtggacc ggacgccgaa gacgaagcgt
      481 ccgtcctggc gcgcgtcgcg cgcgaggacg gcgcgccagc gggggtcggc gccgacgcgg
      541 ggctcgagat cggagggtgc gtgggtcatc gcgagttcgt tcatgtggga tacgataggt
      601 ccgcgcgccg gagcgcacta tccgaaggtt gcgggcgtac ctcgctcagt gccgatagcg
      661 ggccgcgtac acccccaggc accggtcctg cacgacctgt atcccggccg ctgtcagctt
      721 tgctgcggcc gcgtcgttcc ggatcccgct ctgcatccag aacaccttcg gccgacgctc
      781 gggcgggagc gccagaacct cgtcggcgtg cggcgggaca tcttcagagc ggcggaacac
      841 gttcaccaga tcgaacgtgc ccggcacgga ggccaggtcg gggtaggcct tgagcccctg
      901 cgaggactcg atccgcgggt tcacggggac gacctccatc cccgtctcac ggcacatttc
      961 gggaatggtg tacgccggac ggtccgagcg ctgctcgtcc accatgccga tcacggccac
     1021 gcgcttcgcc ccccgcacga tcgcggcgag ggcgtcttcg tcggtcacca caaggctcat
     1081 ccgaagtacc tccggtcgcg tcgagcaggc gaggcagccc attgatcgta accggaaagg
     1141 gctcgactca taccatcttc tggaagatgt tctttgtgcc gtgctgacac gctctcgaca
     1201 gccaaattag gcgctccggc tcagcctcat gcggtggttc ttcggatcga acgtgaccgc
     1261 gaaatcccga agcacccgcg cgccgatgtt tcccacgtcg aagacgggct ggaactcgac
     1321 catcggcttt gcgaaggtcg ttggtcccac ggcaacatcg ccttccagcc ccgcggcctt
     1381 gatgtcgaat tcgttggaga cggtccgggc gtgcccgacc acacggggcg gctcggccag
     1441 ggggagcttc gccgcgaatg attcggggag gctgaagccg cccatggcgc ccgcgtccac
     1501 gtccgtgtcc acgtccacgc ccgcgacgcg catggtcacc gaggggatgc cgcgctcgtc
     1561 acgatagggc acgatgccgg tcccgtccaa cggtagggag ccctgggcca gggtcatccg
     1621 gagcgcgggg aagtccagcg tgagcagaca gtcgctgaag agtccgaaac ccaggatccc
     1681 gtccaccttc tcgccgggca ggcggcccgc gaagtcgccg gtcgtggccg tgagccccgc
     1741 gaagcgggcg tcgccggccg cgagcgagtc gaccgcgacg atctcggcgg tcttcatgtt
     1801 cctgccgctg ggatcgccca cgcgcgcctc gccgacgacg ggaaggccca gcttgcgcgc
     1861 caccgcctcg tcgatgcgga gacaccccgc cgcgcccgtg tcgatcgcga agcggaacgg
     1921 cccctgtccg ttgagcgtca cctccacgga ggccaggtgt ccggcccacg ccatggggac
     1981 caccgcgccg cctgccggaa gcacggtcga ggcgggcttc tcctcgcgcg ccgaatgcac
     2041 ctcggccccg aggggacggg ccgagatcct atggttggcg ccgcccgagg gggcgccggc
     2101 ccgccggagc ca
//
LOCUS       DASSLZ010000202         8971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_707020, whole genome shotgun sequence.
ACCESSION   DASSLZ010000202 DASSLZ010000000
VERSION     DASSLZ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8971
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707020"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2068)
                     /locus_tag="VFM00_07795"
     CDS             complement(<1..2068)
                     /locus_tag="VFM00_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7498189.1"
                     /translation="MALAPGTRLAAYEIMAPLGAGGMGEVYRARDTRLHREVAIKALP
                     TEFARDPQRLARFRREAQTLAGLNHPNIAAIYGLEEEGTTPHLVLELVEGETLAARLK
                     RGPLPSREALAVGIQIASAVEAAHERGIVHRDLKPGNVMLRPDGRAKVLDFGIAKSDS
                     VLEGGDSPHTQTVAERLDATGAGIVLGTAAYMSPEQARGNPVDRRSDVWSFGCVLYEC
                     FSGRPAFAGETTSDLVARILERDPEWRALPDNAPARIREILRRCLRKDAAERPRDIRD
                     VRLELEDVASRGGASGAPETSIAVLPFENLSGVDDEFFADGITEEILNALAQVDGLRV
                     AARASCFAFKGRREDLRSIGERLDVTTVLEGTVRRAGPRLRITVQLANAADGYQLWSE
                     RYDREMTDVFAIQDEIASAIASRLRLALQGDALKGRGTKNLEAYELVLRGRALQARRG
                     RFMAQAARCLERAVALDPEYAEALALLSDSYRLMGSFGVTSMSEAMSRGKSLAERALK
                     IDPELGEAWATLGAFAEQFDREFADSDALYERALRFEPRNARTRAQWALWRLLRGAMA
                     DETARAELRRAVQDDPLNAWVGGMNSFVLGILGSHEEAIVEAERSLQLDPESFFAQWS
                     LMRSHALAGHYDRVVEQAAALLGESGRHPWALGLLAWAHQQSGRRERARACYDELEAR
                     SRHDFMS"
     gene            2296..2682
                     /locus_tag="VFM00_07800"
     CDS             2296..2682
                     /locus_tag="VFM00_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7498190.1"
                     /translation="MDITRSGSQPSAKGSAEYFTGTVRIEPRFGAKDPARVAAVTVTF
                     EPGARTAWHTHPLGQHLIVTSGSGRAQRWGGPVEEIRPGDVVWFPPGEKHWHGAGPTT
                     AMTHLAVQEQLGGKTVDWLEQVSDDA"
     gene            complement(2683..3330)
                     /locus_tag="VFM00_07805"
     CDS             complement(2683..3330)
                     /locus_tag="VFM00_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET7498191.1"
                     /translation="MSKLRVSSFGLSLDGFGAGPDQDLQNPLGVRGPEIMEWFMETRF
                     WRQMHGLEGGETGVDNRMAERGFANLGAWILGRNMFGPVRGPWPDESWKGWWGEEPPY
                     HVPVFVLTHYPRASFEMKGGTVFHFVTEGIQVALDRAQAAAGGRDVRLGGGVSTIRQY
                     LQAGLVDELTLGLRPVLLGKGEHLMQGLDLRALGYQCEESIAGERATHVVLRRRK"
     gene            3515..5884
                     /locus_tag="VFM00_07810"
     CDS             3515..5884
                     /locus_tag="VFM00_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family V/XII"
                     /protein_id="HET7498192.1"
                     /translation="MSSPRMYRFALVALLAAAPSLAAAADLDWPRQFDTPSGSFVIYQ
                     PQPEDLQGDELTGRAAFSLKKTDGTERTYGVLWYTERISIDRDSSTVSARSLDVTKVR
                     LPGITPEEASRYESLVESQARGWDLSLSVEELQAGLAAAEKERASVDDIDNTPPKILF
                     HDRRAILVVYDGDPLLEPIQGSSLQRVVNTPYAVVLDPRSHTYYLNGGNLWYRAKDPL
                     GPWTDISNPPAAVRAVVPPDTLSSDVVEGPPPEVLTATEPTELIATDGPPEYASLVQD
                     ELLYVTNTESDVVLEVETQDLYVLISGRWFRAPSTDGPWTFVRGDELPASFKRVPPDS
                     PKGNILASVAGTDLAEDAMADAEIPQTSAIRRDDQSLQVTYDGDPDFEPIEGTELEYA
                     VNTDAEVILADGRYYACDQGVWYIADDPDGPWSVSDTRPLGLEEIPPSCPVYDVRYVY
                     VYEATPEVVYVGYLPGYLGWYPYYGTVVYGTGYQYNGWRGRHHFYPRRFTWGIHARYN
                     PWLGRWSFGFSYSTGFLRVGSRWRSDASVPRTRTRSAWFGPGGFRRPLVSQDMALLRT
                     RPQRRVRAAALEGLPSNLYNRTENVTRVDRVRNRAIARPIVRPSPVPNDVFGGKDGRV
                     YRREGDGTWQVRTGSEWKPTNVPVLPPATTTQREREETPLPPAKPMPPRESRRRGEPA
                     VERPAPPRNIPTERPVPSRERPEPPRERPMPQEERTTPPRERPAPGNLEREYRARERS
                     NREDKPEAPPPAVRPTLPSEEPKDKKSEEPKKERTTQRLREDVKRDRGR"
     gene            complement(5894..6733)
                     /locus_tag="VFM00_07815"
     CDS             complement(5894..6733)
                     /locus_tag="VFM00_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7498193.1"
                     /translation="MRLGGVLTVAHLTIHEAARRKILLAALIAGAAFLTLFGVGLHFI
                     LRDVSAHETSILERRLFLNIMTLAGLYASSLLTVMTAVLLPVDTLSGEIASGVIQTVA
                     VRPIRRRDIVLGKWLGHWVVMSAYLLLLAGGVLTLTRVLAHFTPPGVLQGLPLMLLEG
                     TVLLTLSIAGGARLSTVTNGILAFGLFGLAFIGNWVEQIGTYADNAAAAQVGTVASLI
                     MPSESLWQLAATQMQPTMLRELGGSPFSPVSVPSAAMVWWAVGYVLLALALGMRAFHR
                     RAL"
     gene            complement(6730..7668)
                     /locus_tag="VFM00_07820"
     CDS             complement(6730..7668)
                     /locus_tag="VFM00_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7498194.1"
                     /translation="MTEHVIETRGLRKQFGAKTAVEDLSLTVRRGEVFGFLGPNGAGK
                     TTSVKMLLGLVAPTSGSASVLGAPLGDRRTRARLGFLPEHFRFQEWLTGRELLHLHAR
                     LLGLRASDGRTRAEALLARVDLLDAAQRPIRTYSKGMMQRVGLAQALLGQPELVFLDE
                     PTSGLDPLGRLLVRDVIHELRERGTTVFLNSHLLGEVEATCSRVSFVKEGRTIHDMAM
                     GEARGRILVELRTGGMASEARETAARFGRVLDGAAPDGEGPQAKLRVEVPGEERLPEL
                     ARALVERGVPLVEMRVAHKSLESWFLEVMGEDQRPG"
     gene            complement(7673..8653)
                     /locus_tag="VFM00_07825"
     CDS             complement(7673..8653)
                     /locus_tag="VFM00_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498195.1"
                     /translation="MDDRMLHEYRRDPDPRFARDLRERLRRQEGRRSPVAGRVVRLAA
                     LAAGVALVAALFTIPSVRVSAQSFLELFRVRRFAAVPFSESRFETLRSLEQGKDHGLL
                     VLDKEETVLDPGPMRYVPSREAASPEAGFAVSAPSYLPEGLVADSVFVEGAGAMRLSV
                     SEAKLRSVLQRLDLNDVEVPTGLDGRWIEVKKPPVVFQKFRSPRNGRAMLVQARSPEV
                     SVPAGWDVERLAEIGLRVLGLDTGEARRIARSTDWRSTLLVPVPMNVSTFRQVTVRGQ
                     SGLLITTSTRGQDGRRREGAMLMWTQGERVFCLQGNLSGRDLMQMAESVS"
     gene            complement(8656..>8971)
                     /locus_tag="VFM00_07830"
     CDS             complement(8656..>8971)
                     /locus_tag="VFM00_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7498196.1"
                     /translation="FAVARNVAIDHFRSARASVPLEEAAGLPGGPTPEEIAEKRSDEA
                     RLGLLLARRPDRERELLALKYGAEHTNRDIARMTGLSESNVGTILHRAVQALRADWDE
                     GA"
BASE COUNT         1500 a         3228 c         2964 g         1279 t
ORIGIN      
        1 gggacatgaa atcatggcgg gagcgcgcct ccagctcgtc gtagcatgcg cgcgcgcgct
       61 cacggcggcc cgactgctgg tgcgcccagg cgagcagacc cagcgcccag ggatgccgtc
      121 cggattcgcc aaggagcgcc gcagcctgct cgaccacgcg atcgtaatga ccggcgagcg
      181 catggctccg catcaggctc cactgagcga agaacgattc gggatccagc tgcagcgacc
      241 gctcggcttc gacgatcgct tcctcatggg atcccaggat tccaaggacg aaggagttca
      301 tgccgccgac ccaggcgttc aacggatcgt cctggacggc gcgccgaagc tccgcgcgcg
      361 ccgtttcgtc cgccatcgcc ccgcggagca acctccagag cgcccactgg gcccgcgtgc
      421 gcgcgttgcg cggctcgaag cgaagcgccc gctcgtacag cgcatcggaa tccgcgaact
      481 cacgatcgaa ctgttcggca aaggccccca gcgtcgccca cgcctcgccc agctccggat
      541 cgattttcag cgcccgctcg gcgagagatt ttccccgcga catcgcttca gacatcgaag
      601 tgacgccgaa cgaacccatc agccggtacg aatccgatag gagtgccaag gcctcggcat
      661 actcgggatc gagcgcgacg gcccgctcga ggcagcgagc cgcctgggcc atgaagcgcc
      721 cgcgccgcgc ctgcagcgcg cgcccgcgca gcaccagctc gtaggcctcg aggttcttcg
      781 tcccgcgccc cttcagcgcg tctccttgaa gggccagccg gaggcgcgag gcgatcgcgc
      841 tggcaatctc gtcctggatc gcgaacacgt ccgtcatctc ccggtcgtaa cgctccgacc
      901 agagctggta accgtcggcg gcgttggcga gctgcaccgt gatccggagg cgcggaccgg
      961 cgcgccgcac cgtcccttcc agcaccgtgg tgacgtccag cctctctccg atcgaccgca
     1021 gatcctcgcg gcggcccttg aacgcgaagc acgacgcccg agccgccacg cgcagaccgt
     1081 ccacctgcgc cagggcgttc aggatctctt cggtgatgcc gtcggcgaag aactcgtcat
     1141 cgacgccgct cagattctcg aacggcagga ccgcgatcga cgtttcggga gcgccggagg
     1201 cgccgcccct cgaggccacg tcttccagct ccagccggac gtcgcggata tcgcggggac
     1261 gctcggcggc gtccttgcgc aggcagcggc gcaggatctc gcggatgcgc gccggagcgt
     1321 tgtcgggaag cgcgcgccat tccggatcgc gctccagaat gcgcgcgacc aggtccgagg
     1381 tggtctctcc cgcgaaggcg gggcgacccg agaagcactc atagaggacg caaccaaagg
     1441 accatacgtc gctccttcgg tcgaccggat tccctcgcgc ctgttccgga ctcatgtacg
     1501 ccgcggtccc gaggacgatg cccgctcccg tcgcgtcgag gcgttccgcc acggtctggg
     1561 tgtgcgggga gtccccgccc tccaggacgg aatcgctctt cgcgatgccg aagtcgagca
     1621 ccttggctct tccatcgggg cggagcatga cgttgccggg cttcaggtcg cgatggacga
     1681 tccctcgctc gtgcgccgcc tcgacggcgc tcgcgatctg gatcccgacc gccagggctt
     1741 cgcgcgacgg gagcggaccc cgcttcaggc gcgccgcgag cgtctccccc tcgaccagct
     1801 ccagcaccag atgcggcgtc gtcccctcct cctcgaggcc atagatcgcg gcgatattgg
     1861 ggtggttgag tccggcgagc gtctgagctt cacggcggaa gcgcgcgagg cgctgggggt
     1921 cgcgggcgaa ctcggtgggc aaggctttga tcgccacctc gcgatgcaat cgcgtgtcgc
     1981 gggcgcggta cacctctccc atgccacccg ccccgagcgg tgccatgatc tcgtacgcgg
     2041 cgagtcgggt gccgggtgca agggccattt gtgggagtcc tgacgccaag ggtcgagcga
     2101 tcggggtccg agagaaggtt acgttaccac aagcgccgaa ggccggccgc gcactcccgc
     2161 gggcgaacgt gcttgtgaaa gccggaacag acaatacgct ttcacgattg cggggcgcgc
     2221 gggattgcta tatccgcaca aagacgggat acaggctaag cgaaacggaa ggcgccttca
     2281 ccaaggagat cgagcgtgga catcacgaga agcggctcgc agccttcagc caagggatcc
     2341 gccgagtact tcaccgggac ggtgcggatc gagccacggt tcggcgccaa ggacccggcg
     2401 cgggtggccg ccgtcaccgt cacgttcgag ccgggcgccc gcacggcgtg gcacacgcac
     2461 ccgctgggcc agcacctgat cgtgacctcc ggtagcggac gcgcgcagcg ctggggcggg
     2521 ccggtcgaag agatccggcc cggcgacgtc gtctggttcc cgccgggcga gaagcactgg
     2581 cacggcgccg ggccgaccac cgccatgacg cacctcgccg tccaggaaca gctcggcggg
     2641 aagacggtcg actggctgga acaggtcagc gacgacgcgt aatcacttcc gccggcgcag
     2701 cacgacgtgc gtcgcgcgct ctcccgcgat cgattcctcg cactggtagc ccagcgcgcg
     2761 cagatcgagg ccctgcatca agtgttctcc cttgcccagg agcaccgggc ggaggcccag
     2821 ggtcagctcg tcgaccagcc cggcctggag gtactggcgg atggtggaca cgccgccgcc
     2881 caggcgcacg tcgcgcccgc ccgcggcggc ctgggcccga tccagcgcga cctgaatgcc
     2941 ctccgtgacg aaatggaaga cggtgcctcc cttcatctcg aaggaagccc gcgggtaatg
     3001 ggtcagcacg aagacaggca cgtgatacgg cggctcctcg ccccaccatc ccttccagct
     3061 ctcgtccggc cacggccccc gcaccgggcc gaacatgttc cgtcccagga tccacgcgcc
     3121 gagattcgcg aagccccgct cggccatgcg attgtcgacg ccggtctcgc cgccctccag
     3181 gccgtgcatc tgccgccaga agcgggtctc catgaaccat tccatgatct cgggcccgcg
     3241 cacgccgagc ggattctgga ggtcctggtc cggcccggcg ccgaagccgt cgagggaaag
     3301 gccgaagctg ctgacgcgaa gtttggacat gccggttctc cgatacgatc gcggtcgatg
     3361 tgttgtcgtc aggccacatt atcacggtca cactcgcccg tggacttcga gacaccatcg
     3421 gaaattcgca agcgcgccac attcccacca catgtcggga gtaccttgta cgtgatagga
     3481 acgagcgtat tcgatcgttc gcctggaggc gtccatgtcc tcgccgcgga tgtaccgctt
     3541 cgcccttgtc gccctgctcg ctgccgcccc ttcgcttgcg gccgccgcgg acctcgactg
     3601 gccgcgccag ttcgacacac cgagcggctc cttcgtcatc tatcagccgc agccggagga
     3661 tctccagggc gacgagctca ccggccgcgc cgccttctcg ctgaagaaga ccgacggcac
     3721 cgagcgtacg tacggggtgc tctggtacac ggagcgcatc tcgatcgacc gcgacagcag
     3781 cacggtgtcc gcgcgcagcc tggacgtcac gaaggtgcgc ttgccgggga tcacgcccga
     3841 agaggcttcg cgctatgaga gcctggtcga atcccaggcc aggggctggg atctatccct
     3901 ttccgtcgaa gagcttcagg ccggcctggc cgccgccgag aaggagcggg ccagcgtgga
     3961 cgacatcgac aacacgcctc ccaagatcct cttccacgac cggcgcgcga tcctggtcgt
     4021 ctacgacggc gatccgctcc tcgagccgat ccagggctcg tcgctccagc gcgtggtgaa
     4081 cacgccctac gccgtggtgc tcgatccgag gagccacacc tactatctga acggcgggaa
     4141 tctctggtat cgggcgaagg acccgctcgg gccgtggacg gatatctcca atcccccggc
     4201 ggccgttcgc gccgtggttc ccccggacac cttgtcgtcc gacgtggtgg agggtccgcc
     4261 gccggaggtg ctgaccgcga ccgagcctac ggagctgatc gccaccgatg gcccgcccga
     4321 gtacgcctcg ctggtccagg acgagctgct ctacgtgacc aataccgaga gcgacgtcgt
     4381 cctggaggtc gagacgcagg acctgtacgt cctgatttcc ggccggtggt tccgggctcc
     4441 ctccaccgac ggcccatgga ccttcgttcg cggcgacgag ctgccggcca gcttcaagcg
     4501 ggtgccgccc gactcgccca aggggaacat cctggcgagc gtcgcgggca cggacctggc
     4561 cgaagacgcg atggccgacg ccgagatccc ccagaccagc gcgatccggc gcgacgacca
     4621 gagcctgcag gtgacgtacg acggcgatcc cgatttcgag ccgatcgagg gcaccgaact
     4681 cgaatacgcc gtgaacaccg acgccgaggt gattctcgcc gacggccgct actacgcgtg
     4741 cgaccagggc gtctggtaca tcgcggacga ccccgacgga ccctggagcg tcagcgacac
     4801 ccggccgctc ggcctggagg agatcccgcc cagctgcccc gtgtacgacg tgcgctacgt
     4861 ctacgtctac gaagcgacgc ccgaagtcgt gtacgtcggc tacctgcccg ggtacctggg
     4921 ctggtatccc tactacggca ccgtcgtcta cgggaccggg taccagtaca acgggtggcg
     4981 gggccggcac catttctatc cgcgtcgctt cacctggggg attcatgccc gctacaaccc
     5041 ctggctaggc cggtggagct tcggcttcag ctacagcacg ggcttcctcc gcgtcggcag
     5101 ccgctggcgc tcggatgcct cggtcccacg cacccgcacc cgatcggcat ggtttggtcc
     5161 gggaggattc cgccgcccgc tggtgtctca ggacatggcg ctgttgcgga cccggcctca
     5221 gcgtcgcgtg cgggccgccg cgttggaagg gctgccgtcc aacctctaca accggacgga
     5281 gaacgtcacg cgcgtcgacc gcgtccgaaa ccgtgcgatc gcgcggccga tcgttcgacc
     5341 ctcgcccgtt cccaacgacg tgttcggggg caaggacggc cgcgtctatc gccgcgaggg
     5401 ggatggaacg tggcaggtcc ggaccggaag cgagtggaag ccgacgaacg tcccggtcct
     5461 tccgccggcc acgacgactc aaagggagcg cgaggaaacg ccgcttcctc cggccaagcc
     5521 catgccgccg agggagagca ggcggcgcgg cgagccggcg gtcgagcgcc cggcgccgcc
     5581 gcgcaacatc ccgacggagc ggcccgtgcc ctcgcgcgag cggcccgagc cgccacggga
     5641 gcggccaatg ccgcaagagg agcgaaccac gcccccacgg gaacgccccg cgcccggcaa
     5701 cctggagcgc gagtaccgcg cgcgcgagcg gtcgaatcgc gaggacaagc cggaggcgcc
     5761 gccgccggcg gtgcgtccga ccctgccatc ggaggagccc aaggacaaga aatcggaaga
     5821 gcccaagaag gaacggacga cgcagcgatt gagggaggat gtgaagcgcg accgcggtcg
     5881 ctgagatcgc gggctacagg gcccgccggt gaaacgccct catgcccagc gcgagcgcca
     5941 ggaggacgta gccgaccgcc caccacacca tcgccgcgct cggaaccgac accggcgaga
     6001 agggcgagcc gcccagctcg cgcaacatcg tgggttgcat ctgcgtcgcg gcaagctgcc
     6061 agagcgattc gctcggcatg atgaggctgg ccacggttcc cacctgcgcc gcggcggcgt
     6121 tgtcggcgta cgtgccgatc tgctccaccc aattcccgat gaaggccagg ccgaagagcc
     6181 cgaaggcgag gatgccgttg gtcacggtgc tcaagcgcgc gccccccgcg atcgaaaggg
     6241 tgaggagcac ggttccctcg agaagcatga gcggcagccc ttggagcacc cccggcggcg
     6301 tgaagtgggc caggactcgc gtgagggtca gaacgcctcc cgcgagcagg agaagatagg
     6361 cgctcatgac gacccagtgg ccgagccact tgcccaggac gatgtcgcgg cggcggatgg
     6421 gccgcaccgc gacggtctgg atgacccccg aagcgatctc gccggagagc gtatcgaccg
     6481 gaaggagcac ggcggtcatg accgtaagga gactggacgc gtagaggccg gccagcgtca
     6541 tgatgttgag gaagaggcgg cgctcgagga tcgacgtctc gtgcgccgac acgtcccgga
     6601 ggatgaaatg aagtcccacg ccgaacagcg tgaggaacgc cgcgccggca atcagggccg
     6661 cgagcaggat tttccgtcgc gcggcctcgt ggatcgtcag atgcgcgacc gtcagcacgc
     6721 cgcccagtct catccaggcc tctgatcttc gcccatcacc tcgaggaacc acgactcgag
     6781 ggacttgtgg gccacgcgca tctcaaccag cggaacgccg cgctcgacga gcgcgcgcgc
     6841 cagctccggc agccgctctt cgccgggtac ctcgacgcgg agcttggcct gcggcccttc
     6901 cccgtcggga gcggcgccgt ccaggacgcg cccgaagcgg gccgccgtct cgcgcgcttc
     6961 cgaggccatc ccacccgtgc gaagctcgac caggatccgc ccccgcgcct cgcccatcgc
     7021 catgtcgtgg atcgtgcgcc cctccttcac gaacgacacg cggctgcagg tggcctcgac
     7081 ctcgccgagc aggtgcgagt tcaggaacac ggtcgttccg cgttcccgta gctcgtggat
     7141 cacgtcccgc accaagagcc gccccaaggg atcgagcccc gacgtcggct cgtccaggaa
     7201 gaccagctcc ggctggccca gcagcgcctg cgccaggccc acccgctgca tcattccctt
     7261 cgagtaggtc cggatgggcc gctgggccgc gtccagcaga tccacgcggg cgagcagcgc
     7321 ctcggcgcgc gtgcggccgt ccgaggcgcg aagcccgagg aggcgcgcat gcaggtgcaa
     7381 cagctcgcgt cccgtcagcc actcctgaaa tcggaagtgt tcggggagaa agcccaaccg
     7441 cgcgcgcgtg cggcgatcgc cgagcggtgc ccccaggacg ctcgccgagc cggacgtggg
     7501 cgcgacgagc cccaggagca tcttgaccga ggtcgtcttc cccgccccgt tcgggccgag
     7561 gaaaccgaag acctctcccc gccgaacggt cagggagagg tcttccaccg cggtcttcgc
     7621 gccgaactgc ttccgcagcc cgcgcgtctc gatgacgtgt tccgtcacca ggctaggaga
     7681 ccgactccgc catctgcatc agatcgcgtc cggagaggtt gccctggagg cagaacaccc
     7741 gctcgccttg cgtccacatc aacatcgctc cctcgcgacg gcgtccgtcc tgtccgcgcg
     7801 tgctcgtcgt gatcagaagg cccgattgtc cgcgcacggt cacctggcgg aacgtgctca
     7861 cgttcatcgg caccggcacg agcaacgtgc tccgccaatc ggtcgagcgc gcgatccgcc
     7921 gtgcctcgcc cgtgtccaaa cccagcaccc gcaacccgat ctcggccagc cgctccacgt
     7981 cccagcccgc cggcaccgag acctcggggc tccgcgcctg caccagcatc gctcttccgt
     8041 tccgcggcga gcggaacttc tggaacacca ccgggggctt cttcacttcg atccagcgcc
     8101 cgtccagccc ggtgggaacc tcgacgtcgt tcaggtccag ccgctggagc accgagcgca
     8161 gtttcgcctc gctgacggac agcctcatgg cccccgcccc ctcgacgaag acgctgtccg
     8221 cgaccagtcc ctcgggcaga tacgaaggcg cgctcaccgc gaacccagcc tccggcgaag
     8281 ccgcctcccg cgagggaacg tagcgcatgg gacccgggtc gagcaccgtc tcctctttgt
     8341 cgagaacgag gagcccgtgg tccttcccct gttccaggga gcgcagcgtc tcgaaccgcg
     8401 attcgctgaa cgggacggcg gcgaaccgac gcacccggaa cagctcgagg aacgactggg
     8461 cggagacgcg caccgagggg atggtgaaga gcgcggccac gagcgcgacg ccggcggcga
     8521 gggcggccag gcgcaccacg cgccctgcga cgggcgagcg ccgaccctcc tgccgccgca
     8581 gccgctcgcg gagatcgcgc gcaaagcgcg gatcgggatc gcggcggtat tcgtgaagca
     8641 tccgatcgtc catcgtcagg ctccttcatc ccagtcggcg cgcagggcct gcaccgcccg
     8701 gtgcaggatg gttccgacgt tcgattcgct caggcccgtc atgcgggcga tgtcccggtt
     8761 cgtgtgctcc gcgccgtact tcaacgccag gagctcgcgc tcgcgatcgg gccgccgcgc
     8821 cagaagcagg ccaaggcgtg cttcgtccga gcgcttctcg gcgatctctt cgggcgtggg
     8881 tcctcccgga agcccggccg cctcctcgag aggaacagaa gcgcgcgcac tccggaagtg
     8941 gtcgatcgcc acgttccggg ccaccgcgaa g
//
LOCUS       DASSLZ010000203         4521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1332692, whole genome shotgun sequence.
ACCESSION   DASSLZ010000203 DASSLZ010000000
VERSION     DASSLZ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332692"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..2578
                     /locus_tag="VFM00_07835"
     CDS             164..2578
                     /locus_tag="VFM00_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HET7498197.1"
                     /translation="MRPSTAPYAATPEPRADRSDSTRERYRRYRRAVDYIAAAQIYLR
                     DNPLLQEPLRPEHIKDRLLGHWGTAPGINTVYAHLNRLVVERDANLMLVTGPGHGAAA
                     NMANLWLEGTLAEFYPRLSRDEAGLRELFREFSWPYGFPSHLSPSIPGVIHEGGELGY
                     ALATSFGAVLDSPGLVVACIVGDGEAETGPTAGAWHSAKFINPESDGAVLPILLQNGF
                     KISNPTIVATMTEEEETSLFRGYGYEPRYVDDGPELDPDMSAAMDWAHDEIARIQRLA
                     RGGTPEARPRWPMLIYRMPKGRGCPPELDGERIAGTFRAHQVPVKDPKNNPKHLDALE
                     RWLRSYRPEELFDAQGRPDPGLIALCPHGSRRIALNSASFGGDVRQPLALPPLERFAV
                     KVERRGTPMVSHMGPFGDYLAEVLVENESARNFRIVCPDELESNRLSAVLRVTDRQYC
                     WPVPEGSAHIARYGRVLEVLSEHNCQGWLEGYLLTGRHGLFPCYEAFIPIVDGMMNQY
                     AKFLKASLEVPWRKPVASLNYLLTSEAWRQDHNGFSHQGPGFINNLLTKKGETYHIFL
                     PPDANTLLVTMEHCFQSTQHINLVVAGKQPMPQWLTLEEAREEFRVGASVWRWASSNE
                     GEDPQVVLAASGDNLTLESIAAAQILREETPDWRVRVVNVMNLLALGIPQKYPHGLDE
                     QRFQRIFPITAPVIYNFHGYTAAIKQLCWERPMNERFDVNGYREEGSTTTPFDMHLRN
                     RTSRWHVVIQAAQKIADRYPSAAARAEELIRRYERKIAEHRDYVVEHGVDPPEIAATR
                     WQDMKL"
     gene            2593..3714
                     /locus_tag="VFM00_07840"
     CDS             2593..3714
                     /locus_tag="VFM00_07840"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HET7498198.1"
                     /translation="MAGAFILCVNAGSSTLKGSLLEASADGGVAEVKGRSVTIDRVPS
                     AEALDEALAALGVSGPEALCAVGHRVVHGGTEFREPVRVDESVKAAIARLSVLAPLHN
                     PPALAAIQAAERRFPNVPHVAAFDTAFFADLPPERVVYSLPYEWYERWGVRRFGFHGL
                     SHQYASGRAQAMLGGGRQRVVVLHLGNGCSASAVVEGNPVATTMGFTPMEGLMMGTRS
                     GSVDPGILLYATREKGLTVEALDDALNHRSGLLGVSGVSGDFRAVAAAAGEGHERACL
                     ALAIYADRARAVVGSLATAMGGIDALVFTAGVGEHAASMRADICRGLEFLGLAIDLYR
                     NAPAVPDADVSLAGGTGRILVIQAREDVMIARAARAAIG"
     gene            3810..4409
                     /locus_tag="VFM00_07845"
     CDS             3810..4409
                     /locus_tag="VFM00_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7498199.1"
                     /translation="MRSSSRALIADRAQLRAIASPARQEILDLLVRTGAASAAELGRL
                     VGRPADGLYYHLRALERVGLIRTDGFEAGAGRKGARYRAVHKEPAIRHDAAPGGNSRD
                     VTAIVASMLRLGVRDFRRASALPGVRTREPGRELWALRVTGRLTPAQVSTANRGIRAL
                     RDTVGQPGAKGKLYAITILLTPLEHRARKRQRRPRKKRS"
BASE COUNT          700 a         1551 c         1593 g          677 t
ORIGIN      
        1 gtggtcgata cgcgaaaggt gctgattcgt tagagacagg cacgaggggt cgggccgggc
       61 gccgtgtgct ccggcccgcc tcgcgcgcgc tcaggccgcg ggaccatttg attttcggga
      121 tcgggaggca gagcatggag cggttgcaga aaggagccgt ctcatgcgcc catccaccgc
      181 accgtatgcc gcaacgcccg aaccgcgcgc cgaccgttcg gactcaaccc gggagcgcta
      241 ccgccgttac cgccgcgccg tcgactacat cgccgcggcg cagatctatc ttcgcgacaa
      301 tccactgctc caagaaccgc tccggcccga gcacatcaag gatcgcctgc tcggccactg
      361 gggcaccgcg cccggcatca acacggtcta cgcccacctg aaccggctgg tcgtggagcg
      421 cgacgccaac ctcatgctgg tcacgggacc gggacatggg gcagccgcca acatggccaa
      481 cctctggctc gaggggacgc tcgccgagtt ctaccccagg ctctcgcgcg acgaggcggg
      541 gcttcgcgag ctgttccgcg agttctcctg gccgtacgga tttcccagcc acctgagccc
      601 cagcatcccg ggcgtgattc acgaaggagg cgagctggga tacgcgctcg cgacctcctt
      661 cggggcggtg ctggacagcc ccgggctggt cgtcgcctgc atcgtcggcg acggcgaggc
      721 ggagaccggc ccgaccgccg gagcgtggca cagcgccaag ttcatcaatc ccgagtcgga
      781 cggggcggtg ctcccgatcc ttctgcagaa cgggttcaag atctcgaatc cgacgatcgt
      841 ggccacgatg accgaagagg aggagacatc gctctttcga ggctacggct acgagccgcg
      901 ctacgtggac gacgggccgg agctggaccc ggacatgagc gccgcgatgg actgggcgca
      961 cgacgagatc gcgcggatcc agcggctcgc gcgcggcggc acgcccgagg cgcgcccgcg
     1021 ctggccgatg ctgatctatc gcatgccgaa gggacgcggc tgccctccgg agctggacgg
     1081 cgagcggatc gcggggacct tccgcgcgca ccaggtgccg gtgaaggatc ccaagaacaa
     1141 tccgaagcac ctggacgcgc tggagcgttg gctccgctcc taccggccgg aagagctgtt
     1201 cgacgcgcag ggacgccccg atcccggcct gatcgccctc tgcccgcacg gatcgcggcg
     1261 gatcgcgctg aactccgctt ccttcggagg ggacgtgcgg cagccgctcg cgctgccccc
     1321 gctcgagcgc ttcgcggtca aggtggagcg gcgcggaacg ccgatggtga gccacatggg
     1381 cccgttcggc gactacctgg ccgaggtgct ggtcgagaac gagagcgcgc gcaactttcg
     1441 gatcgtctgt cccgacgagc tggaatcgaa ccggctctcg gcggtgctgc gcgtgaccga
     1501 ccggcagtac tgctggccgg tcccggaggg ctcggcgcac atcgcccgct acggccgcgt
     1561 actcgaggtg ctttccgagc acaactgcca ggggtggctg gagggctatc tcctgacggg
     1621 ccggcatggg ttgttcccat gctacgaagc cttcatcccg atcgtggacg ggatgatgaa
     1681 ccagtacgcc aagttcctga aggcatcgct ggaggtgccg tggcggaagc cggtcgcctc
     1741 cctcaactat ctgctcacct ccgaggcgtg gcgccaggat cacaacggct tctcgcacca
     1801 ggggcccggc ttcatcaaca acctgctcac gaagaaggga gagacctacc acatcttcct
     1861 cccgcccgac gcgaacacgc tgctcgtcac catggagcac tgcttccagt cgacgcagca
     1921 catcaacctc gtggtcgcgg gcaagcagcc gatgccgcag tggctcacac tggaggaggc
     1981 gcgggaggag ttccgggtgg gcgcgagcgt gtggcggtgg gccagctcga acgagggaga
     2041 agacccgcag gtggtgctcg cggccagcgg cgacaatctc accctggaat cgatcgcggc
     2101 cgcgcagatc ctgcgcgagg agacgcccga ctggcgggtg cgggtcgtca acgtcatgaa
     2161 cctgctcgcg ctggggattc cccagaagta tccgcacggc ctggacgagc agcgcttcca
     2221 gcggatcttc ccgatcacgg cgccggtcat ctacaacttc cacggctata cggccgccat
     2281 caagcagctc tgctgggaac ggccgatgaa cgagcgcttc gacgtgaacg ggtatcgcga
     2341 ggagggctcg accacgacgc ccttcgacat gcatctccgg aatcgcacga gccgctggca
     2401 cgtggtgatc caggcggccc agaagatcgc cgaccgctat ccgtcggccg cggcccgcgc
     2461 ggaggagctg atccgccgct atgagcggaa gatcgcggag caccgcgact acgtcgtgga
     2521 gcacggggtg gacccgcccg agatcgccgc gacgcgctgg caggacatga agctctagcc
     2581 gagggcgcgg tcgtggccgg cgcattcatc ctctgcgtca acgcgggatc gagcacgctc
     2641 aagggctctt tgctcgaggc ctccgcggac ggcggcgtcg cggaggtcaa gggacgaagc
     2701 gtcacgatcg atcgcgtccc gagcgcggaa gcgctggacg aggcgctggc cgccctaggg
     2761 gtgtcgggtc cggaggccct atgcgcggtc gggcaccgcg tggtgcatgg cgggacggag
     2821 tttcgcgagc cggtgcgcgt cgatgaaagc gtcaaggcgg cgatcgcgcg gctctcggtg
     2881 ctcgcgcccc tccacaatcc ccccgcgctc gccgcgatcc aggccgcgga gcggcggttt
     2941 ccgaacgtcc cgcacgtcgc cgcgttcgat acggcgttct tcgccgatct ccccccggaa
     3001 cgagtcgtct attccctccc ctacgaatgg tacgaacgct ggggcgtccg gcggttcggt
     3061 ttccacggcc tgagccacca gtacgcctcc ggccgcgccc aggccatgct ggggggcggg
     3121 cggcagcggg tcgtggtgct tcaccttggc aacggctgct cggcgagcgc cgtggtggag
     3181 ggaaaccccg tcgcgaccac catgggcttc acgccgatgg aggggctgat gatgggcacc
     3241 cgctccggct cggtggaccc ggggatcctc ctctatgcga cgcgtgagaa aggcctcacg
     3301 gtcgaagcgc tggacgacgc cttgaaccac cggtcggggc tgctcggtgt ttccggcgtc
     3361 tccggcgatt tccgcgcggt cgcggcggcc gcgggcgagg gtcacgagag ggcgtgcctc
     3421 gcgctggcga tctacgccga ccgggcgcgc gcggtcgtgg ggagcctcgc caccgccatg
     3481 gggggaatcg acgcgctggt cttcacggcc ggggtcgggg agcatgccgc gtcgatgcgg
     3541 gccgacatct gccgcgggct cgagttcctg ggactggcga tcgacctgta ccgaaacgca
     3601 cccgccgtgc ccgacgcgga cgtctcgctc gcgggtggta cgggccgcat cctcgtgatc
     3661 caggctcgcg aggacgtgat gatcgcgcgg gcggcgcgcg ccgcgatcgg ctagagaaga
     3721 agcccgcggc ggatggcggc agggcggggt ccggctgata ttgaaaattg ctattgtttt
     3781 tcattaatta gtggcgcacg cttcttctca tgcgctcttc ctcccgcgcc ctcatcgccg
     3841 atcgcgccca gctccgggcg atcgcttccc cggcccgcca ggagatcctc gacctcctcg
     3901 tccggacggg ggccgcgtcg gcggcggagc tgggccggct ggtcggccgt cccgccgacg
     3961 ggctgtacta ccacctccgc gcgctcgagc gggtcgggct gatccgcacc gacggcttcg
     4021 aagcgggggc gggccggaag ggggcgcgct accgcgccgt ccacaaagag cccgcgatcc
     4081 gccacgacgc cgcaccggga ggcaactcgc gggacgtcac cgccatcgtc gcgtccatgc
     4141 tccgcctggg ggtccgcgac ttccggcgtg cctcggcgct ccccggggtg cgcacgcggg
     4201 agccgggtcg cgagctatgg gcgcttcgtg tcacggggcg gctcacgccg gcccaggtga
     4261 gcacggccaa tcgaggcatc cgcgcgctgc gcgatacggt cggacagccc ggcgccaagg
     4321 ggaagctcta cgcgatcacg atcctgctca cgccgctcga gcaccgcgcg cggaagcggc
     4381 agcgccggcc gcggaagaag cgctcatgaa gctcgcttcg ttcgctctgg ccgtttcgct
     4441 gggagccggc ctggcacccg ccgcgccgtc gcgggcgctt gccgcggggg ctgccaagca
     4501 gggcacggca tttctgacgt a
//
LOCUS       DASSLZ010000204         6220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1596830, whole genome shotgun sequence.
ACCESSION   DASSLZ010000204 DASSLZ010000000
VERSION     DASSLZ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596830"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VFM00_07850"
     CDS             <1..1161
                     /locus_tag="VFM00_07850"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HET7498200.1"
                     /translation="EILAGMHDGQFVVDAYQAGAGTSFHMNVNEVIANRAAELLGGER
                     GDTKLVSPNDHVNMAQSTNDVIPTAIRLAAYVLTGPVVEEMQALARAFGERARAFSGV
                     LKSGRTHLQDAVPITLGQEFSGYEVAIDGWAESLKRSRAGLLPLGLGGNAAGTGINAH
                     PKYRERAVAQLASMMNAPFVPAPNLFEAMQSMAPLVRLSNDLRGFALDLTRIANDLRL
                     LASGPTTGFNEIVLPAVQPGSSIMPGKVNPVMLEMTNMVCFQVLGYDATVAYAAQAGQ
                     LELNVMMPVIAHNLLRSLHILAPAMKVLREKCVDGITANEEVCRGYFDRSISVATALN
                     PYIGYLAAAEVAKESAKTGKTVVQIVRERKLLTEEQIAKAFSPEGMTHPGGN"
     gene            1161..1706
                     /locus_tag="VFM00_07855"
     CDS             1161..1706
                     /locus_tag="VFM00_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498201.1"
                     /translation="MGADLGSTVRAARALRAVFVLLLAALALPACSKRDGRVPESSAA
                     PVDTARIKAAEVASWPYRREISADLDGDKKLDRVVIAADVPTGPTGPLWQDAQRWAVY
                     VEPEKGDRTLLYGALVSHGFAEAAVLDPDDKDHRLVLIQERTPEQVRAMEVEYRKGKA
                     KLVSAAAYRIGEWLPGAASMR"
     gene            complement(1794..2399)
                     /locus_tag="VFM00_07860"
     CDS             complement(1794..2399)
                     /locus_tag="VFM00_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7498202.1"
                     /translation="MKRNLVLVLMAVVLMAVASTAGAATRPHHESSSNPGTLELNLGY
                     AKSSEDMGGGDTMGGGLAFGAAYWLHPSAAVTWGPEFSYDGLGSVSYDNGFTTNNEAS
                     MHLWRVHPTIRYTFTKGVGTQFFGQAGAGLYNVTAKIDDSVLGNASASDSKFGFNLGA
                     GVDFQVSEKTRMNVTGLYHDVATSGSSLNYMQFRAGVAFNL"
     gene            complement(2494..3105)
                     /locus_tag="VFM00_07865"
     CDS             complement(2494..3105)
                     /locus_tag="VFM00_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7498203.1"
                     /translation="MKRNLALALATAVVGLALAPLASASPVPSLGTVELNAGYSKSST
                     DVTSGVDIGGAENAMGGGLSFGAGYWRSASPMISWGLEAGYDNLGTANYDNGTTTDNE
                     IKTNAFRVNPALRFNFGAGVGPSFYAQGGAGLYSVSADIKDSVAGDVSDSQSKFGFNV
                     GAGVSFPVGPKSRMNLTGLYHTVSTEGQSLNYIQFRAGVGFGL"
     gene            complement(3286..4608)
                     /locus_tag="VFM00_07870"
     CDS             complement(3286..4608)
                     /locus_tag="VFM00_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7498204.1"
                     /translation="MHRALGSVVREYVEQVRHSGRNARLYLAGLFLIGFGQSIFSLLF
                     NLYLRALGFSDAGIGQIISKISLGAAVAAIPAAFLFRSIPARVILVGAGALCAAISAM
                     QAALTEPELMLVVAFISGMALTVYRLSIAPVVMGEVAEPARPFLFSAAFTVLVFAAIL
                     GSAIGGFLPHLFHLITPSDRLALRASLFVSAAATLLCAIPFHRMKDPGRIASGPEPPA
                     PLAPGVFVERRLRGAWRQVRDLAELDWGLNLKLVAPAMLIGLGAGLIIPFLNLYFRDR
                     FGLSEAKIGLLFSAMQAFMVAGNLFGPAVSRRMGLVRGVVVTQLVSVPFMVALAVSSW
                     FPLVAASFFLRSALMNMNQPLSSHFAMEVVPKRDHAITNSLLSLSWYLAWSVSADVGG
                     VLIERKGYTEPLLIAAALYVAASALYWYFFRNVEEGRLPRAEVEIPSA"
     gene            complement(4612..5460)
                     /locus_tag="VFM00_07875"
     CDS             complement(4612..5460)
                     /locus_tag="VFM00_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7498205.1"
                     /translation="MPELSAGGDVPELSAGGRLHVRSVEGALAVLGGVYANHRALRAV
                     LEDARDRGAVRIFCLGDLGGFGPNPDRVFPILEEYGVETVAGNYDRSLAARLADCGCG
                     YAHPRDNAYARRSYEYTNRCTTDPQRRWLAALPSEIRFTRGGKRWLLCHGSPRRVNEF
                     LWDSATSDRFLDRLMRESGADQILCTHTGLHWQRTLPGGGRVVNVGAIGRPANDGRTE
                     VWYAFFEPGAEAACFVPVAYDHEALAREMEDEALPEEFVETIRTGWWTTCLEILPAKE
                     RARGRF"
     gene            complement(5448..>6220)
                     /locus_tag="VFM00_07880"
     CDS             complement(5448..>6220)
                     /locus_tag="VFM00_07880"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7498206.1"
                     /translation="FIACGPANHDHELMTRDEIRPYLLEAESLGVNDLYFTGGEPFLN
                     RELLAILEDALQIGPATVLTNGTLITEERARALAEAHEASRYSLEVRVSLDGTTPERN
                     DPIRGAGSFDQAMEGVERLAAAGLNPILTVARTWGDEEDDAIRERFHDLLRARGIARP
                     RVKVLPLFLIGREPERTRGYGAEELLTEEHMRGHDPWSLQCSTSRMVTSRGVMVCPIL
                     IDAPDALMGKTLRESMRAHPLAHGACHTCWATGATCRN"
BASE COUNT         1036 a         2131 c         2147 g          906 t
ORIGIN      
        1 gagatcctgg ccggcatgca cgacgggcag ttcgtcgtgg acgcctacca ggcgggcgcc
       61 ggcacgtcgt tccacatgaa cgtgaacgaa gtgatcgcca atcgcgccgc cgagctcctg
      121 ggcggcgagc ggggcgacac caagctcgtg agccccaacg atcacgtgaa catggcgcag
      181 tccacgaacg acgtgatccc caccgccatc cgcctcgccg cctacgtgct gaccggcccc
      241 gtcgtcgagg agatgcaggc gctggctcgc gcgttcggcg agcgggcgcg cgccttctcg
      301 ggcgtcctga aatcgggacg cacgcacctt caggacgcgg tgccgatcac gctcggccag
      361 gagttcagcg gctacgaggt cgcgatcgac ggctgggccg agtcgttgaa gcggagccgc
      421 gcggggctgc tcccgctggg tctcggcggg aacgccgcgg gcaccgggat caacgcgcac
      481 ccgaagtacc gcgagcgggc cgtggcccag ctcgcctcca tgatgaacgc tccctttgtt
      541 ccggcgccga atctgttcga ggcgatgcaa tcgatggcgc ccctcgtccg gctctcgaac
      601 gaccttcgcg gcttcgcgct cgacctgacg cgaatcgcga acgacctgcg gctcctcgcg
      661 agcgggccga cgaccggctt caacgagatc gtccttcccg ccgtgcagcc gggatcctcg
      721 atcatgccgg gcaaggtgaa tccggtgatg ctcgagatga cgaacatggt ctgcttccag
      781 gtgctgggct acgacgccac ggtggcctat gccgcccagg cgggccagct ggagctgaac
      841 gtcatgatgc cggtgatcgc ccacaacttg ctccgctcgc tccacatcct cgccccggcg
      901 atgaaggtcc tgcgcgagaa gtgcgtcgac gggatcaccg ccaacgaaga ggtctgccgc
      961 ggctacttcg accgctcgat ctcggtcgcg acggcgctga acccctacat cggctacctc
     1021 gcggccgccg aggtcgcgaa ggagtcggcg aagacgggaa aaaccgtcgt ccagatcgtg
     1081 cgggagcgga agctcctcac cgaggagcag atcgcgaagg cattctcccc cgaagggatg
     1141 acgcacccgg gcggcaactg atgggcgccg acctcggttc gacggtgagg gcggcgcggg
     1201 ctctccgcgc cgtcttcgtt ctgctcctcg cggcgctcgc ccttcccgcc tgctccaagc
     1261 gcgacgggcg cgtcccggaa tcgtcggcgg cccccgtgga cacggcgcgc atcaaggccg
     1321 ccgaggtcgc gtcctggccc taccgccgcg agatctcggc cgacttggac ggcgacaaga
     1381 agctggaccg cgtcgtgatc gcggcggatg tgccgacggg cccgacgggg ccgttgtggc
     1441 aggacgccca gcgctgggcg gtctacgtgg agcccgagaa gggggaccgg acgctcctgt
     1501 acggcgccct ggtgtcccac ggcttcgccg aggcggccgt acttgatccc gacgacaagg
     1561 accaccggct ggtgctgatc caggagcgca cgccggagca ggtgcgggcg atggaagtgg
     1621 agtaccgcaa ggggaaggcc aagctcgtct ccgccgccgc ctaccggatc ggggagtggc
     1681 tccccggcgc ggcgtcgatg cgatagcgcc cgcgcgcgcc gaaggcgcgg caccaacgaa
     1741 atcggggcgg cgcctcgcgg cggccgcccc gtttcatgaa acccggctgg ggattacagg
     1801 ttgaaggcga cccccgcccg gaactgcatg tagttcaggc tcgagccgct cgtcgcgacg
     1861 tcgtggtaga ggccggtcac gttcatgcgc gtcttctcgc tgacctggaa gtccacgccc
     1921 gcgcccaggt tgaatccgaa cttggaatcg gaggccgacg cgttgcccag cacgctgtcg
     1981 tcgatcttgg ccgtcacgtt gtacagccca gcgcccgcct gcccgaagaa ctgcgtgccg
     2041 acgcccttcg tgaacgtgta gcggatcgtc gggtgcacgc gccacaggtg catcgacgcc
     2101 tcgttgttcg tggtgaagcc gttgtcatag gaaacgctgc ccaggccgtc gtaggagaac
     2161 tccggacccc acgtgaccgc cgcggacgga tgcagccagt acgccgcgcc gaaggccagc
     2221 ccgccgccca tcgtgtctcc accacccatg tcttccgacg acttcgcgta gcccaggttc
     2281 agctcgagcg tgcccgggtt cgagctcgac tcgtggtgcg gacgcgtcgc ggcgcccgcc
     2341 gtggaggcga cggccatcag gaccaccgcc atcaggacca gaacgagatt gcgcttcatc
     2401 gtgtgctcca tgggttcgag agtagagccg ttcatgagaa tggggcggcg ccgacgggcg
     2461 ccgccccgac ttcctgctgc cacgaaactg cgattacagg ccgaagccga cgccggcgcg
     2521 gaactggatg tagttcaggc tctggccttc ggtcgacacc gtgtgataga gacccgtcag
     2581 gttcatgcgc gacttcgggc cgaccgggaa gctgacgccc gcgccgacgt tgaagccgaa
     2641 cttggactgg ctgtcgctga cgtcgccggc gacgctgtcc ttgatgtccg ccgacacgct
     2701 gtacagaccg gcgccgccct gggcgtagaa cgaggggccg acgcccgcgc cgaagttgaa
     2761 ccggagggcc ggattcacac ggaacgcgtt cgtcttgatc tcgttgtcgg tcgtcgtgcc
     2821 gttgtcgtag ttcgccgtgc cgaggttgtc atacccggcc tcgaggcccc acgaaatcat
     2881 cggcgacgcg ctgcgccagt aacccgcacc gaaggacaga ccgccgccca tcgcgttctc
     2941 cgcgccgccg atatcgacgc cggaagtcac gtccgtcgag gacttggagt agcccgcgtt
     3001 gagctcgacc gttcccaggc tgggcaccgg agacgcgcta gcgagcggcg cgagcgccag
     3061 accgacaacc gcagtcgcaa gagccagagc cagattccgc ttcattgctt cccccttctg
     3121 tttgtagacg gacttccgat tccacccact gggcggccgt tggatcggca tccatgccgc
     3181 ggcgcccacc accccgaccg acattgaatc ggcatgtgaa ggcgggaact tgagggggtg
     3241 caccgaaggg gacggaacgt gggtaaaccg attcactact gaagtttaag cgcttggaat
     3301 ttcgacctcg gcccttggca gacggccctc ttcgacgttc cggaagaagt accaatagag
     3361 ggcggaggcg gccacgtaga gcgcggccgc gatcaggagc ggctcggtgt agcccttccg
     3421 ctcgatcagg acgccgccca cgtcggcgct cacgctccag gccaggtacc aggacaggga
     3481 gagcaggctg ttcgtgatgg cgtggtcgcg cttgggcacc acttccatcg cgaagtggga
     3541 ggacagcggc tggttcatgt tcatgagcgc gctccggagg aagaaggagg cggccacgag
     3601 cggaaaccag ctcgacaccg ccagcgccac catgaacggc accgacacga gctgggtcac
     3661 gaccaccccg cgcaccagcc ccatgcgccg cgagaccgcg ggcccgaaga ggttccccgc
     3721 caccatgaag gcctgcatcg cgctgaagag aagtccgatc ttggcctccg acaggccgaa
     3781 gcggtcccgg aagtagaggt tcaggaacgg aatgatgagc cccgcgccca gcccgatcag
     3841 catcgccggg gcgaccagtt tgagattcag gccccagtcc agctccgcga gatcgcgcac
     3901 ctggcgccag gcgccgcgga ggcgccgctc gacgaagaca ccgggcgcga ggggggccgg
     3961 aggttccggg ccggacgcga tccggcccgg gtccttcatc cggtggaacg ggatcgcgca
     4021 gagcagcgtg gcggcggccg agacgaagag cgatgcgcgc agggccagcc ggtcggaggg
     4081 cgtgatcagg tggaagaggt gcggcaggaa tcccccgatc gccgagccga ggatggccgc
     4141 gaacacgagc acggtgaagg cggcgctgaa gaggaagggg cgggcgggct cggccacctc
     4201 gcccatcacg accggcgcga tcgaaagacg atagaccgtg agcgccatgc ccgagatgaa
     4261 cgccacgacc agcatcagct cgggttcggt cagggccgcc tgcatggcgc tgatcgcggc
     4321 gcagagcgcg ccggcgccga ccaggatcac gcgggccggg atcgagcgga agaggaacgc
     4381 ggccggaatc gccgcgacgg ccgcgccgag cgagatcttg gagatgatct gcccgatccc
     4441 cgcgtcggag aagcccagcg cgcgcaggta gaggttgaag aggagggaga agatcgactg
     4501 cccgaagccg atcaggaaga gcccggccag atagagccgg gcgttccggc cggaatgccg
     4561 gacctgctcg acgtactcgc gaacgacgct cccgagcgcg cggtgcatcc gctagaagcg
     4621 tccccgggcg cgctccttcg cgggcaggat ctccaggcag gtcgtccacc agcccgtgcg
     4681 gatcgtttcc acgaactcct ccgggagcgc ctcgtcctcc atctcgcgcg ccagcgcttc
     4741 gtgatcgtac gcgaccggta cgaagcatgc ggcctcggcg ccgggctcga agaaggcgta
     4801 ccacacctcg gtgcgcccgt cgttggcggg acggccgatc gcgcccacgt tcacgacgcg
     4861 gccgccgccc gggagggtgc gttgccagtg gagcccggtg tgggtgcaga ggatctggtc
     4921 ggcgcccgat tcccgcatca gccggtcgag gaagcggtcc gaggtggccg agtcccacag
     4981 gaactcgttc acgcgccgcg gcgagccgtg gcagaggagc cagcgcttcc cgcctcgcgt
     5041 gaagcggatc tccgagggca gcgccgccag ccatcggcgc tgcgggtcgg tggtgcagcg
     5101 attcgtgtat tcataggaac gccgcgcgta ggcgttgtcg cggggatggg cgtagccgca
     5161 gccgcagtcg gcgaggcgcg ccgcgagcga gcggtcgtag ttccccgcca cggtctcgac
     5221 cccgtactcc tcgaggatcg ggaatacccg atcggggttg ggcccgaagc cgcccaggtc
     5281 tcccagacag aagatgcgga cggcgccgcg atcccgcgcg tcctccagca cggcccggag
     5341 cgcccggtga ttggcataga cgccgccgag cacggcgagc gccccttcga cgctccgtac
     5401 gtggaggcgg cccccggcgc tcagttccgg cacgtcgccc ccggcgctca gttccggcac
     5461 gtcgcccccg tcgcccagca ggtgtggcag gctccgtgcg ccagaggatg cgcgcgcatc
     5521 gattcccgca gcgtcttccc catgagggcg tcgggcgcgt cgatcaggat cgggcagacc
     5581 atgacgcccc ggctggtcac catgcggctc gtggagcact ggagggacca gggatcgtgt
     5641 ccgcgcatat gctcctcggt caggagctcc tcggcgccat agccgcgcgt ccgttcgggc
     5701 tcccttccga tcaagaagag cgggagcact ttcacgcgcg gcctcgcgat cccgcgcgcg
     5761 cgcaggagat cgtggaagcg ctcccggatc gcgtcgtcct cctcgtcccc ccacgtgcgc
     5821 gccacggtga ggatcgggtt cagcccggcc gccgccagcc gttccacgcc ctccatcgcc
     5881 tggtcgaacg agcccgcgcc gcgaatcgga tcgttccgct cgggcgtggt cccgtccagg
     5941 ctcacgcgca cctccagcga atagcgcgag gcttcgtgtg cctcggcaag cgcgcgcgcc
     6001 cgctcctcgg tgatcagcgt gccgttggtc aacacggtcg cgggcccgat ctggagcgcg
     6061 tcttccagga tcgccagcag ctcgcggttc aagaacggct ccccgccggt gaaatagagg
     6121 tcgttcacgc ccagggactc cgcctcgagc agatagggac ggatctcgtc gcgcgtcatc
     6181 agctcgtgat cgtgattggc gggcccgcac gcgatgaagc
//
LOCUS       DASSLZ010000205         6317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1839129, whole genome shotgun sequence.
ACCESSION   DASSLZ010000205 DASSLZ010000000
VERSION     DASSLZ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839129"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFM00_07885"
     CDS             complement(<1..498)
                     /locus_tag="VFM00_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET7498207.1"
                     /translation="MPHAAFRAFVDAHDDFLILSHIDPDGDAVGSSLGLSWALRPIGK
                     RVTVANDSPLPASLRFLPGWEEVRLASELPGPFGAVFVLDCSSLDRVGRSGIGAIAPG
                     AAIACVDHHAGTDGFGDPRLVNVSASATAELIVEILQACELPIGPEAAACLYAGIASD
                     TGAFRF"
     gene            complement(505..876)
                     /gene="rbfA"
                     /locus_tag="VFM00_07890"
     CDS             complement(505..876)
                     /gene="rbfA"
                     /locus_tag="VFM00_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET7498208.1"
                     /translation="MPARSESPRTRRVAERIQVELAEILSKETEDPRLRSLSVTSARV
                     SRDLASARVWVSGRMTPDEEKSVLAALEHAAPFFRTLLAPRLGLRIVPTLRFEVDRTI
                     EAGARIEELLREIKDQDETKE"
     gene            complement(941..1207)
                     /locus_tag="VFM00_07895"
     CDS             complement(941..1207)
                     /locus_tag="VFM00_07895"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET7498209.1"
                     /translation="MRLELHLPASDSLKAKRSVLNRVKERVRSGFNASVAEVEYQDLW
                     QRAALGIAIVGLAPGALDNVLRDILRAVEREDRLQVLDFQIRII"
     gene            complement(1224..3395)
                     /gene="infB"
                     /locus_tag="VFM00_07900"
     CDS             complement(1224..3395)
                     /gene="infB"
                     /locus_tag="VFM00_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET7498210.1"
                     /translation="MKRRVYEAAKDMGMSSEALVSILKSMSVDVKSHMSSIDDNVVEQ
                     VRLKIARQKEAVKEEEARRKEKAVQAAKAAAEAQRRSAPAGAGLLKGGKGVKKKKRIV
                     DEKLVRASVRRTLAEMDGSKRRHHRRDEKEDAAIGVEEEPRVIRVSEFISVAELAAQL
                     EVKPQEVIAACMRLGIMANINRRLDKDSIQAVADEFDYGVEFVDELGEETVVETAVEE
                     HLVPRAPVVTIMGHVDHGKTSLLDHIRKANVIAGEAGGITQHIGAYEVSLPAGQKITF
                     LDTPGHEAFTAMRARGAQVTDIVVLVVAADDQVMPQTIEAIDHARAAQVPIIVAINKI
                     DLPDANVEKVRQDLAQQNLLVEEWGGKTIAVPISAKKGTNVEKLLEMILLQAEVLELK
                     ADPTKHARGVVVESRVEQGRGIVATVLVQKGTLKVGDAFVAGATSGKVRALLNERGQR
                     IAQAGPSAPAEVLGFSTAPQAGDTFAAVDDEREARELAAKRGQLLREQEFRLHKHVTL
                     TDLYSQMQRGMGELLVVLKGDVDGSVEALQDSLQKLSTDEVKLRVIHRGVGQITESDV
                     LLAAASNAIIIGFHVRPDPRAAELASKEKVDIRLYEIIYEAVENVKDAMSGLLRPEIR
                     ESIAGTAEVRKVFRTTKSGAIAGCMVLSGTITRNARARLLRGGEAIYDGKIESLKRFK
                     DDVREVASGYECGIGLEDRDDIREGDVIEAYVLEEVARKLA"
     gene            complement(3425..5062)
                     /gene="nusA"
                     /locus_tag="VFM00_07905"
     CDS             complement(3425..5062)
                     /gene="nusA"
                     /locus_tag="VFM00_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET7498211.1"
                     /translation="MNYELMDAMAQIAREKSVDKSILIETLVAGLLQAGKKRFGPEAD
                     VDVKFDEQSGKIVMALRRTVVEDADDLGREVDLAEAQSQDPDAKIGQVLVQELSLEDL
                     GRNAIATAKQVLVQRVREAERERIYEDFHDRVGEMVRGTVQQVDRGNIYVKLDRSEAI
                     LPPREQIARDRFHQGDHIKAYIIAVDKLARGPQVILSRTHPEFLRRLFESEVPEVAEK
                     IIEIKGIAREPGSRAKVSVVSNDDKIDAVGACVGIKGSRVQAIVRELGGEKIDIVPWS
                     VDPIVFVTRALSPAKVLEAHVVEMEQKILVTVADDQLSLAIGKGGQNARLAAKLTGWK
                     IDLISKSERERQKEYERRSRVDIEEFEAAGPKLREKLLREGIETVQDLLKTPLEELLA
                     IQGVGEKTAEKLLEEARELLARRGEELEAEAARAVDEAEKQKAAEAAAIARLQEMEAA
                     DRVEEAEAETVREASMETEVDEQMRGDDSIGENEAVQVGDVETMTIDEGEEDHLRPVE
                     DEAASDEAAADETDQDEDETREERATGAESEGPPPRA"
     gene            complement(5092..5601)
                     /gene="rimP"
                     /locus_tag="VFM00_07910"
     CDS             complement(5092..5601)
                     /gene="rimP"
                     /locus_tag="VFM00_07910"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET7498212.1"
                     /translation="MDRNQLRDDLSRRLRALLEEEAFDLWDLEVTFQSGRTVVQVTVE
                     RPGGGVTLDECAYWNRKLGRYLEAENVLPGSYVLEVGSPGIERTLTRPEHYARYVGSS
                     LEVKLHDPREGRRTYRGELRAAGPESIVVEDSEAGTVSLPHAAIRKSHLIVDPWEGMR
                     GKDRHKKEH"
     gene            complement(5840..>6317)
                     /locus_tag="VFM00_07915"
     CDS             complement(5840..>6317)
                     /locus_tag="VFM00_07915"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET7498213.1"
                     /translation="LHRHAPGIGVDIALFDGEDLGTDTNPAGFFRGSRRYLEWRATEP
                     APLFVVVIDMVGRKDAAFYWEGNSYNRASNIVLLLWNRAKDMGLRQFRNGVKFTIADD
                     HIPFLEADIPAIDVIDFDFPAWHTHKDDLSAVDPGTLEAMGRVLLSLVTNVDYLQN"
BASE COUNT          878 a         2277 c         2055 g         1107 t
ORIGIN      
        1 gaaccggaac gcgccggtgt ccgaggcgat gccggcgtag aggcaggccg ccgcctcggg
       61 gccgatcggt agctcgcagg cctggaggat ctccacgatc agctcggcgg tggcgctcgc
      121 gctcacgttc accaggcgcg gatcgccgaa cccgtcggtg ccggcgtggt ggtccacgca
      181 ggcgatggcg gctcccggcg cgatcgcccc gatgcccgaa cggcccacgc ggtcgagcga
      241 cgagcagtcg agcacgaaga cggcgccgaa cggtccgggg agctccgacg cgaggcggac
      301 ctcctcccag cccggcagga agcgcaggct ggccgggagc ggcgaatcgt tcgccacggt
      361 gacgcgcttt ccgatcggcc ggagcgccca ggaaagtccg agcgacgagc ccacggcgtc
      421 gccgtcggga tcgatgtggg agaggatcag gaaatcgtcg tgcgcgtcca cgaaggcgcg
      481 aaaggcggca tgcggcaagc ggcgttactc cttggtctcg tcctggtcct tgatttcgcg
      541 gagcagctcc tcgatccgcg cgcccgcctc gatggtgcgg tccacctcga agcgcagcgt
      601 gggcacgatg cgcagcccca gccgcggcgc gagcagcgtg cggaagaagg gcgcggcgtg
      661 ctccagcgcg gcgagcacgg acttctcctc gtcgggcgtc atccgcccgc tcacccagac
      721 ccgcgccgaa gcgaggtcgc gggagacgcg cgccgaggtc acgctcagcg agcgcaggcg
      781 cggatcctcc gtctccttgg acaggatctc ggccagttcg acctggatgc gctcggcgac
      841 gcggcgcgtg cgcggactct cggagcgtgc gggcaacgat ctctcctctg ggactcggga
      901 ccgggccgtg cgggccgcgg aatcccggga gccccgggct tcaaatgatc cggatctgga
      961 agtcgaggac ttgcagacgg tcctcgcgct cgacggcgcg gaggatgtcc cgcagcacgt
     1021 tgtcgagggc gccgggtgcc aaccccacga tggcgatccc cagggccgcc cgctgccaga
     1081 ggtcctggta ctccacctcg gcgaccgaag cgttgaagcc cgagcgcacg cgctccttca
     1141 cccggttcag cacggagcgc ttggccttca gggaatcgga ggcgggcagg tggagctcga
     1201 gccgcatcgt cccgaccacc atgtcacgcc agcttgcgcg cgacttcctc gagcacgtac
     1261 gcctcgatga cgtccccttc ccggatgtcg tcgcgatcct cgagcccgat gccgcactcg
     1321 tagccgctcg ccacctcgcg aacgtcgtcc ttgaagcgct tcagcgactc gatcttcccg
     1381 tcgtaaatcg cctcgccccc gcgcaggagc cgggcgcgcg cgttccgggt gatcgtgccc
     1441 gagagcacca tgcagccggc gatggcgccc gacttcgtgg tgcggaagac cttccggacc
     1501 tcggccgtac ccgcgatcga ttcgcggatc tccggacgca ggagccccga catggcgtcc
     1561 ttcacgttct ccaccgcctc gtagatgatc tcgtagaggc ggatgtccac cttctccttc
     1621 gacgccagct cggcggcgcg gggatcgggg cgcacgtgga agccgatgat gatcgcgttc
     1681 gacgccgcgg ccagcagcac gtccgactcg gtgatctgcc cgacgccgcg atggatcacg
     1741 cggagcttca cctcgtccgt cgagagcttc tgcagcgagt cctggagcgc ctcgaccgag
     1801 ccgtccacgt cgcccttcag cacgacgagc agctcgccca tgccgcgctg catctggctg
     1861 tacaggtcgg tcagggtcac gtgcttgtgg aggcggaact cctgctcgcg cagcagctgg
     1921 ccgcgcttgg ccgcgagctc gcgcgcctcg cgctcgtcgt ccacggccgc gaacgtgtcg
     1981 ccggcctgcg gcgccgtcga gaagcccagc acctccgccg gcgcgctcgg gcccgcctgg
     2041 gcgatccgct gaccgcgctc gttcaagagc gcgcgcacct tgccgctcgt ggcgcccgcg
     2101 acgaaggcgt cgccgacctt gagcgttccc ttctgcacga gcaccgtcgc gacgatgccg
     2161 cgcccctgct ccacgcgcga ctccacgacc acgccgcggg cgtgcttggt gggatcggcc
     2221 ttcagctcga ggacctcggc ctgcaggagg atcatctcga gcagcttctc gacgttggtg
     2281 cccttcttgg cggagatcgg aacggcgatg gtcttgccgc cccattcctc gaccaggagg
     2341 ttctgctgcg ccaggtcctg gcgcaccttc tcgacgttgg cgtcgggaag gtcgatcttg
     2401 ttgatcgcga cgatgatcgg gacctgcgcg gcgcgggcgt ggtcgatcgc ctcgatggtc
     2461 tggggcatga cctggtcgtc cgcggccacc accagcacca cgatgtcggt gacctgggcg
     2521 ccgcgggcgc gcatcgccgt gaaggcctcg tggcccggcg tgtcgaggaa cgtgatcttc
     2581 tgcccggcgg gcagcgagac ctcgtaggcg ccgatgtgct gcgtgatgcc gcccgcctcg
     2641 cccgcgatga cgttcgcctt ccggatgtgg tcgaggagcg acgtcttgcc gtggtccacg
     2701 tgccccatga tcgtgacgac cggggcgcgc ggaacgagat gctcttcgac ggccgtctcg
     2761 acgaccgttt cctcgcccag ctcgtccacg aactccacgc cgtaatcgaa ctcgtcggcg
     2821 accgcctgga tcgagtcctt gtcgaggcgg cggttgatgt tcgccatgat gccgaggcgc
     2881 atgcacgcgg cgatcacctc ctgcggcttc acttcgagct gggcggccag ctcggccacg
     2941 gagatgaact cgctcacgcg gatgacccgc ggctcttcct cgacgccgat cgcggcgtcc
     3001 tccttctcgt cgcggcggtg atggcggcgc ttggaaccgt ccatctcggc gagcgtgcgc
     3061 cggacggagg cgcggaccag cttctcgtcc acgatccgct tcttcttctt gacgcccttg
     3121 ccgcccttca agagacccgc gccggcgggc gcggaacggc gctgcgcctc ggcggcggcc
     3181 ttggccgcct gaacggcctt ctccttgcgg cgcgcctcct cctccttcac ggcctccttc
     3241 tggcgggcga tcttcagccg cacctgctcc acgacgttgt cgtcgatcga gctcatgtgg
     3301 ctcttcacgt ccaccgacat ggacttgagg atggagacga gcgcctcgct cgacatcccc
     3361 atgtccttgg ccgcttcgta cacccgccgc ttcacgcctg ccatacgtct cctcgttctc
     3421 gcccctacgc ccggggcggg ggtccttccg attccgcgcc cgtggcgcgt tcctcgcgcg
     3481 tctcgtcctc gtcctgatcg gtctcgtccg cggccgcctc gtccgacgcc gcctcatcct
     3541 cgacggggcg gaggtggtcc tcctccccct cgtcgatcgt catcgtctcg acgtcgccga
     3601 cctggacggc ctcgttctcg ccgatggagt cgtcgccgcg catctgctcg tcgacctcgg
     3661 tctccatcga cgcctcgcgg accgtctcgg cttcggcctc ctcgacgcgg tcggccgctt
     3721 ccatctcttg gagccgcgcg atcgccgcgg cctcggccgc cttctgcttc tcggcctcgt
     3781 ccaccgcacg cgccgcttcg gcctcgagct cctcgccccg gcgcgccaac agctcgcgcg
     3841 cctcctcgag cagcttctcc gccgtcttct cgcccacgcc ctggatggcg agcagctcct
     3901 ccagcggggt cttcagcaga tcctgcaccg tctcgatccc ctcgcgcagc agcttctcgc
     3961 gcagcttggg accggcggcc tcgaactcct cgatgtccac gcgcgagcgg cgctcgtatt
     4021 ccttctgccg ctcccgctcg ctcttggaga tgaggtcgat cttccaaccc gtcagcttgg
     4081 ccgcgagccg cgcgttctgg ccccctttgc cgatcgccag cgacagctgg tcgtcggcca
     4141 cggtgaccag gatcttctgc tccatctcga ccacgtgcgc ctcgagcacc ttggcgggcg
     4201 agagcgcacg cgtgacgaac acgatcgggt ccacgctcca gggcacgatg tcgatcttct
     4261 cgccccccag ctcgcgcacg atcgcctgca cgcgggatcc cttgatcccg acgcaggcgc
     4321 cgaccgcgtc gatcttgtcg tcgttggaga ccacggagac cttggcccgc gagccgggct
     4381 cgcgcgcgat ccccttgatc tcgatgatct tctccgccac ctccgggacc tcggactcga
     4441 acagccggcg caggaactcc ggatgggtcc gcgacaggat gacctgcggc ccgcgcgcca
     4501 gcttgtccac ggcgatgatg tacgccttga tgtggtcgcc ctggtggaat cggtcgcgcg
     4561 cgatctgctc gcgcggcggg aggatcgcct ccgaccggtc gagcttcacg tagatgttgc
     4621 cgcggtccac ctgctgcacc gtcccgcgca ccatctcgcc cacgcggtcg tgaaagtcct
     4681 cgtagatccg ctcccgctcg gcctcgcgca cgcgctgcac cagcacctgc ttggcggtgg
     4741 cgatggcgtt ccggcccagg tcctccagcg agagctcctg gaccagcacc tggccgatct
     4801 tcgcgtcggg gtcctgcgac tgggcctcgg ccaggtccac ctcgcggccg aggtcgtccg
     4861 cgtcctcgac gaccgtccgg cggagcgcca tgacgatctt gccgctctgc tcgtcgaact
     4921 tgacgtcgac gtcggcctcg gggccgaagc gcttcttccc ggcctggagg agccccgcca
     4981 cgagcgtctc gatgaggatg gacttgtcga cgctcttttc gcgcgcgatc tgagccatcg
     5041 catccatcag ctcgtagttc atgttcttca cttgaggctc gcgagccacg atcaatgttc
     5101 ctttttatgg cggtccttgc cgcgcattcc ttcccaagga tcgacgatca ggtgcgactt
     5161 gcggatcgcc gcgtgcggca gcgacaccgt tcccgcttcc gaatcctcga cgacgatcga
     5221 ttccgggccc gccgcgcgaa gctcgccgcg gtaggttcgc cggccctcgc gggggtcgtg
     5281 cagcttgact tcgagcgagc tgccgacgta ccgcgcgtag tgctcagggc gcgtcagcgt
     5341 ccgctcgatg ccggggctgc ccacctccag cacgtacgag ccgggcagga cgttctcggc
     5401 ctcgagatag cgcccgagct tccggttcca ataggcgcat tcgtccagcg tcacgccgcc
     5461 cccggggcgt tccaccgtga cctgcaccac cgtccgtccc gactggaatg tgacttccag
     5521 gtcccacagg tcgaacgcct cctcctccag aagagcgcgg aggcgccggg agaggtcatc
     5581 tcgaagctgg tttcggtcca tttctagccc ctgggcgctc ccgagcccgg gcacggcccg
     5641 aaaaaggacc gagtggacgg gtgggtccac tcggacctct gaagctaagg tgggtgaggt
     5701 ttggggtcaa gcggcaatgt ggggagtgct ctatataagg atgcaggtag ggggtctggc
     5761 gatgtcccgc cgcccgcagg cggcggaacc ccggtgcctt gtacgcggcc gcgcggaacc
     5821 gagaagcagt tacatctaac taattttgaa gataatccac gttcgtcacg aggctaagca
     5881 gcaccctgcc catcgcttcc agggtccccg gatccacggc cgataggtcg tccttgtggg
     5941 tgtgccaggc cgggaagtcg aagtcgatca cgtcgatggc ggggatgtcg gcctccagga
     6001 agggaatgtg gtcgtcggcg atcgtgaact tgacgccgtt ccggaactga cgaagtccca
     6061 tgtccttggc ccgattccag agcagcagaa cgatgttgga cgcccggttg tacgagtttc
     6121 cctcccagta gaaggcggcg tccttccgcc ccaccatgtc gatcaccacc acgaagaggg
     6181 gcgccggctc ggtggcgcgc cactcgagat agcgccggga gccgcggaag aacccggccg
     6241 gattggtgtc ggtgcccagg tcttccccgt cgaagagcgc tatgtccacg ccgatcccgg
     6301 gggcgtgacg gtgcagc
//
LOCUS       DASSLZ010000206         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1075471, whole genome shotgun sequence.
ACCESSION   DASSLZ010000206 DASSLZ010000000
VERSION     DASSLZ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075471"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFM00_07920"
     CDS             complement(<1..224)
                     /locus_tag="VFM00_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498214.1"
                     /translation="MTRRRIGWSGWWGLAGIAASLVLGAVPAGAVSVSPRVLEAARQD
                     PKAAAALKERLGRWEASKSGGVDRAYPQFAL"
     gene            complement(250..1827)
                     /gene="guaA"
                     /locus_tag="VFM00_07925"
     CDS             complement(250..1827)
                     /gene="guaA"
                     /locus_tag="VFM00_07925"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET7498215.1"
                     /translation="MNPATQKPAPRTTHERLVILDFGSQFTQLIARRAREMGVFAEIY
                     GPHIAPEELKRLAPKAIVLSGGPSSVLERGAPQIPKGALDLGAPVLGVCYGMQLLARD
                     LGGDVRPSARREYGMATLEVAKECALLKGVPRESRVWASHGDTVARLPEGFERLASTE
                     TVVEAAAQDVARSLYVVQFHPEVAHTAHGSQILRNFLFDICGFRGDWTMGQVLEEQIG
                     KIRAQVGSGRVICALSGGVDSSVVAALLHRAIPGQVRAVFVDHGLLRHREAEQVVESM
                     RAVIGEDLVPTDAKARFYEALRDVEDPEEKRKIIGRIFIEIFEETAARLGPPDFDFLA
                     QGTLYPDVIESTSTRGPSAKIKSHHNVGGLPERMKMELVEPLRELFKDEVRELGALLG
                     LPKAVLGRHPFPGPGLAVRILGPVSPEAVETLTQADDIFIHELRSSGEYERVWQAFAV
                     LLPVRTVGVMGDGRTYDQVVALRAVTSSDGMTADWARLPDALLARVSSRIVNEVKGVN
                     RVVYDISTKPPATIEWE"
BASE COUNT          385 a          798 c          760 g          352 t
ORIGIN      
        1 agagcgaact gcggataggc gcggtcgacg ccgccgctct tggacgcctc ccaccggccc
       61 aggcgctcct tcaacgcggc cgcggccttg ggatcctggc gcgcggcttc gagcacgcgc
      121 ggcgagacgc tgaccgcgcc cgcgggcacg gcgccaagaa ccaacgacgc ggcgatcccg
      181 gcaagccccc accaccccga ccaaccgatt cgacgacggg tcactgacga ctccttcctg
      241 gtccgccggt cattcccact cgatcgtggc cggaggcttg gtgctgatgt catagacgac
      301 gcgattcacg cccttcacct cgttcacgat gcgcgaggag acgcgggcca gcagggcatc
      361 gggaagccgc gcccagtccg ccgtcatgcc gtcgctcgag gtcacggcgc ggagcgccac
      421 cacttggtcg taggtccggc catctcccat cacacccacg gtccggaccg gcaggagcac
      481 ggcgaaggcc tgccagacgc gctcgtactc ccccgaggac cgaagctcgt ggatgaagat
      541 atcgtccgct tgggtcaggg tctccaccgc ttccggcgat accggcccca gaatccggac
      601 cgcgaggccg ggccccggga aggggtggcg gcccagaacg gccttgggaa gccccagaag
      661 cgcccccagc tctcgaacct cgtccttgaa taactcacga aggggctcca ccagttccat
      721 tttcatgcgc tcggggaggc cgcccacgtt gtgatgggac ttgatcttgg ccgaagggcc
      781 acgggttgag gtgctctcga tcacgtcggg atagagggtg ccctgggcca ggaaatcgaa
      841 atccggaggt cccaggcgtg cggccgtctc ttcgaagatc tctataaaga tgcggccgat
      901 gatctttcgt ttctcctcgg gatcttccac gtcgcgaagg gcctcgtaga accgcgcctt
      961 cgcgtccgtg gggacgaggt cctcgccgat gaccgcgcgc atcgattcca ccacctgctc
     1021 ggcctcgcga tggcgcagga ggccgtgatc cacgaagacc gcccggacct gccccgggat
     1081 cgcccggtgg aggagggccg ccacgaccga cgaatccacg ccgcccgaga gcgcgcagat
     1141 gacgcggccc gatccgacct gggcccggat cttcccgatc tgctcctcca gcacctgccc
     1201 catggtccag tcgccgcgga acccgcagat gtcgaagagg aaattgcgga ggatctggct
     1261 cccatgggcg gtgtgggcca cctcgggatg gaactggacg acgtagaggc tccgcgcgac
     1321 gtcctgggcg gccgcctcga ccaccgtctc cgtgctcgcg agccgctcga acccctcggg
     1381 aagcctagcc accgtgtcgc catggctcgc ccagacccgg ctctcgcgcg ggaccccctt
     1441 caggagcgcg cactccttgg ccacctcgag ggtggccatg ccgtactccc gccgcgcgct
     1501 cggccggacg tccccgccca ggtcgcgagc caggagctgc atgccgtagc agaccccgag
     1561 caccggggcg ccgaggtcga gcgcgccctt cggaatctgc ggcgcccccc gctccagcac
     1621 gctggagggg ccgcccgaga ggacgatcgc cttcggcgcc agtcgcttca gctcctcggg
     1681 cgcgatgtgc gggccgtaga tctcggcgaa gacccccatc tcccgcgcgc ggcgcgcgat
     1741 gagctgcgtg aactgggagc cgaagtcgag gatgacgagg cgttcgtggg tcgtgcgcgg
     1801 ggccggtttc tgcgtggcgg ggttcatctc gagaaggttg gaagcggtgg gatcattcag
     1861 ggggcgatcc tccttcgatg aaatgggctg tcgatggcga gactcggagc atatcgcacg
     1921 ggacaccgag ccgacaagct accgaacggg atcggggatc gcttcgcctg aaaaccagct
     1981 ctcgtcctta tcggaggagg tatatgccgt ccaatggccc cgatggtagg cgccatccgg
     2041 gcggtcggag agaatcgaaa agccgggtag cgcacggccg ggtgcgatcc cgcgattcgg
     2101 atcggtggcg atccattcga agaatccgcg gtcttccccg gccatcacga acttcacggt
     2161 ccacccgcgc ggggacctgc aggaggcctc aacggccttc cgccccggga ggcgacggat
     2221 atacgccaac ctcaggtcct ggtcgtagcc gaccgcgaat ttggagatcg gcgccttcga
     2281 gccattgaag atccg
//
LOCUS       DASSLZ010000207        12647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_964272, whole genome shotgun sequence.
ACCESSION   DASSLZ010000207 DASSLZ010000000
VERSION     DASSLZ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12647
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964272"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2960
                     /locus_tag="VFM00_07930"
     CDS             <1..2960
                     /locus_tag="VFM00_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7498216.1"
                     /translation="VEQLRAQEFVTPSEEPAAPSRREFLKLAGAGAAFLAAGCARKPV
                     EKILPYGKAPEDMQPGNPVYYASTCGECPAGCGLLVKTREGRPIKLEGNPDHPANRGA
                     LCARGQASVINLYDPDRLRGPVTVDRATGKTSAAGWGPVDKRVTMALRKARDGGGRVV
                     LLTGTVASPTTRALIHDFVAAFPAAEHVAYDAVSNDAAARAGEATYGDRSLPRYRLDR
                     ADVLVTLGADPLGTFGDTVEQGRDFAKRRRPESGAMSRVIAVESILTLTGTNADRRVR
                     VRPDQLYPFAMALASRLGAGGAGYTPEAVEAAADLPSGTIASIADELAQARGRSLVMA
                     DPNAAPAAHAVALQIAVNLINSALGNDGVTVDASRASGAVHGSGEEMLRLVERMRAGE
                     IQALIVYGTNPAYSLPTAVGFADALKRVPFVASMADRADETARLADVVAPSTHYLESW
                     GDHEPRQGVLSLMQPAIAPLYDVRSFQDSLLAWARYLGRGPLAETAGSYHEYLRERWR
                     TQVYPGAGAAAPFDLFWEGALRTGVIAREENADRTASGRSPRAGGLGGSLPVAATPEN
                     GALTLVLYPSATLGDGRSGNNAWLQELPDPVSKICWDNFAAISPHRARQLGVRDYEMK
                     ADVVTVDVGHGRFDIPVHVQPGLHDDVVAIMLGYGRTGAGTVGNRVGQNAFALAEATG
                     GQVGLAGIPVRVSRAGTVVPIACVQGHQYTEGRPIIYETTLSEYQRDPSAGNEGGELL
                     PSMWSRHKYPGHKWGMAIDLNACTGCSACMLACMVENNVPAVGKAQVLRGREMHWLRI
                     DRYYSGPAEDPETVHQPMLCQHCENAPCETVCPVLATVHSSEGLNLQVYNRCVGTRYC
                     SNNCPYKVRRFNWFDYTDKPKSLRLVLNPDVTVRTKGIMEKCTFCVQRIRDAREHSKV
                     LGVPIQEGDLQTACQQTCPSQAITFGDQNDPKSRVADMARNKRSYHVLADLNTLPSIA
                     YMTKVRNREEESA"
     gene            2993..4402
                     /gene="nrfD"
                     /locus_tag="VFM00_07935"
     CDS             2993..4402
                     /gene="nrfD"
                     /locus_tag="VFM00_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7498217.1"
                     /translation="MASHAPATLPLAEPVLINPTKGFDDVTREVARPIGEKPSRLWWT
                     FFGISVLLTAQFAAEITYQIVKGIGIWGLNHPVGWAVDITSFVFWIGIGHAGTLISAI
                     LHLFRQRWRTAINRSAEAMTIFAVMTAGLFPLIHTGRPWFAYWLIPYPNWRHLWVNFR
                     SPLVWDVFAVSTYLTISLCFWYTGLIPDFATLRDRTSGWKRGIYSLLSLGWRGSERHW
                     QHYERAYMMLAGLSTPLVLSVHSVVSFDFATSILPGWHTTIFPPYFVAGAIFSGFAMV
                     VTLLVILRKVFHLEEYVTLHHLENMNKIILATGSMVGYAYATEFFVAMYSGNQFESFT
                     FMNRATGPFAWAYWTMITCNVIIPQLFWFRKLRRSLPVMFVISLFVNVGMWFERFNII
                     VTSLHRDFLPVNWSYYKPTIFDYSVIVGSFGFFFMWFLLFCRFFPTISIAEVKSVLWS
                     AKKATGNVPAAGHAERTHG"
     gene            4395..4934
                     /locus_tag="VFM00_07940"
     CDS             4395..4934
                     /locus_tag="VFM00_07940"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HET7498218.1"
                     /translation="MVSLFGKPKPLHGVLALYDNPDTLLNAAAQVREHGFTKADAYTP
                     YPVHGLSEALGIRKSWVPYVTLVMGLSGAILGLAFEIWTSAVDWPINVGGKPFVSLPA
                     FIPITFECGVLLGGTMTLAAMLVACGLPNFRDPILDRSLTDDRFAIFIADSGRGWNES
                     RAIQILQGSGAVDVRVIRA"
     gene            4960..5586
                     /locus_tag="VFM00_07945"
     CDS             4960..5586
                     /locus_tag="VFM00_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7498219.1"
                     /translation="MAAVLAAGALIASASGCQKRQPALEYMPNMAYGPRVAAQAEDPL
                     RPGYSVERPPVEGTVPRDLTPYRFAQADTTQAMAALVNPLPRTAEVIGRGQRVYMNTC
                     VVCHGPRGDGQGYIEPLYPQPPLLFSDKVRNWPDARIFHIITRGQNLMPSYASQILPE
                     DRWAVVYYVRALQRAARPLPQDLPAKARAAGADSTGAAANTTGRSASR"
     gene            5583..6797
                     /locus_tag="VFM00_07950"
     CDS             5583..6797
                     /locus_tag="VFM00_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HET7498220.1"
                     /translation="MTAPASAHAAPVRAAAPLSIPGGIRAMFGAFILIGIATFLIEYH
                     GGGDQTRAYAAFLLGYVYFLFIAVAGVFFTALHYLVGATWSVVVRRVAEAFTSFLPYA
                     LLLFVVLLVGIPKLYVWSNPTVAGEVAAEHVTKGGYLSVPFFTARNLVILAVWTLFGW
                     YFVRNSTRQDATRDPVLTKKSLRVAPAFIVIFAITLTLLGFDLLMSLEPLWYSTMFGV
                     YCFAGAWQSCLALLTIAIVLLRRQGALQGIVSRFHYHDLGKYVFAFSIFWMYIAFSQF
                     MLIWYANLPEETEWMIPRTYTGWGIIGIALMVCKFGIPFFALMPVKVKESEAGLLTVC
                     AIVLVGQWLDLFWVILPVFSPERVVFGWTEIGVTLGFLGLFGWIILGFLSRHPVSPSG
                     DPHYESSVRFHG"
     gene            6794..7885
                     /locus_tag="VFM00_07955"
     CDS             6794..7885
                     /locus_tag="VFM00_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7498221.1"
                     /translation="MTEHATPQEPAQGSAGSVVGTSEHGSGAGHGPPRHTAAPDRDKL
                     SSTVAVAQFVFTGVILAVVAGLMYTAGFHSGKGGGEGGAPGAGGGAAPATSVDVQALL
                     KPTPELVNQGKTLFSVNCASCHGTNGKGDGPASSALNPKPRDFTSGYWRYGGGVARIV
                     QTITNGSPGTAMAPFPGIPIQDRFALAQYVRTFNPSKQEADKPEDLAWLDQFGGGAKK
                     DTTGGAAVAAGPAPGDTIPIARAMQLIAERPAPALPAMAGAPADSSEGGMLYAARCAS
                     CHGAWGQGGVRTRMIGSAPYVYVTTRSLAATGPLAGDAAGFERLMVYGLTGGMHPSHG
                     DLTSEQIRVLYDYTQSLRARLSSAARPRS"
     gene            7887..8597
                     /gene="coxB"
                     /locus_tag="VFM00_07960"
     CDS             7887..8597
                     /gene="coxB"
                     /locus_tag="VFM00_07960"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7498222.1"
                     /translation="MISMPTRRTWLAILGLAVGLLAAGTAGAWLLHPATPVAHEVRTL
                     WYKAVAVTSFFLILAEGLLLIVVLRFRAKPGVKAATFHENFRLEMLWTAIPALALVYL
                     AGPSFSTLKYLETVPKSDLTVEIIGHQWFWEYRYPGYGVVFANEPLVIPAGKIVAADV
                     TSIDVVHSWFLPDFGVKMDANPGRVNHTWFQVAKPGTYNGQCAELCGVLHAEMFITVN
                     VVPPEEFARWIEQKKRGA"
     gene            8602..10215
                     /gene="ctaD"
                     /locus_tag="VFM00_07965"
     CDS             8602..10215
                     /gene="ctaD"
                     /locus_tag="VFM00_07965"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET7498223.1"
                     /translation="MEAGTLTRPVGTELVHVGPKPRTGIMKYLLTTDHKLIGVMYIWL
                     SFTFFFIAGIFAMVMRAQLSKAGLHLLHPETYNQIMSLHGTFMVFFWTIPVLAGISNY
                     VVPIQIGARDMAFPRLNAISFWLLIPAGGFMLTSLLLQGGAAAAGWTNYPPLSDRQFS
                     PGPGVDLWILGLHLAGLSSILGGINFVVTILNLRAPGMSIMKMPMFCWNVLITQAVIL
                     IATPVLAGGLTMLLTDRLFGTGFFHASAGGDPVLWQHLFWFYSHPAVYIMVLPAMGMV
                     SQVLPAFSDKRIFGYTGMVIATAGIGFIGFLVWGHHMFVTGIDQRLRFFFSFASMVIA
                     VPTGIKIWSWLATVWGGAIRFRTPMLFGLGFICLFIIGGITGVWLALVPFDVQVHDTY
                     FVVAHLHYVLFGGSIMGIFAGFYYWFPKISGRLYNEFWGKVHFWLVFVGMNVVFMPMH
                     VMGMLGMPRRIYTYKPQYEGLNQISTIGAGILFVAMSIFLINVLMTLWRGKRAPVDPW
                     DHAEYNKTLEWTISSPPPAENFARIPVIR"
     gene            10250..11173
                     /locus_tag="VFM00_07970"
     CDS             10250..11173
                     /locus_tag="VFM00_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET7498224.1"
                     /translation="MSAANDRLVPLLRLTLLLVFVLVVWGGIVRLSGSGLAIPDWPLA
                     HGKLIPKPQHNVLIEYIHRAIAGLVSLGTLGIAFLVFRSARWRARLLPLTLAALITLA
                     LQVFMGARVVLEELPVDRVVAHLVIAFLFFAILIRMALVARDAEDEAGASPAAAAGEG
                     GAADPGKRRGIALWARIAAGAVLAQVALGGWVSSSGAALACPDFPTCQGMWLPPMHGL
                     LGIHYAHRLGAYAVTLIILAMLFRSAPLALPPRARWPLRISGILLTIQFLLGVANVLL
                     RVPLPVSASHLGTALILFGTLNVASYELSRA"
     gene            11166..12035
                     /locus_tag="VFM00_07975"
     CDS             11166..12035
                     /locus_tag="VFM00_07975"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET7498225.1"
                     /translation="MRDYIELTKPRIVALVMITALAGVVAERSLLSDPLRLLVVLGAI
                     MLTAGSANAFNQYFERDLDAQMERTRARRPLPRHRVAPGRALAFAIAIGVLSVVALAQ
                     YGSLLAAWLAFGTIAFYGFFYTLWLKPRTVHNIVIGGAAGAMGPVIAWAAAAGHLALA
                     PVLMFLVIFLWTPPHFWALAICVQDDYRRVSIPMLPIVKGEAETYRQIELYTLVLVLV
                     TLLMPFANAGGLVLGVTALFLGAWFVVKAVRARRAPTVAAARGVFAYSIVYLFVLFLG
                     MIADALWRIPLRS"
     gene            12037..>12647
                     /locus_tag="VFM00_07980"
     CDS             12037..>12647
                     /locus_tag="VFM00_07980"
                     /inference="COORDINATES: protein motif:HMM:NF016337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET7498226.1"
                     /translation="MEPIADAPDPPIRRRIPNWVMGVALGTVVLLMFSFAYANVPLFK
                     LFCARFGIGGSGKAAFQSVYGEGPTLSSSTGPIQSRDIQVKFMGVSATGLPVRFGPSV
                     PLVHVIPGRPVHLDYTFTNMSDDSVFFRAVHSIAPIEAAREFQLIQCFCFEDQALGPR
                     ETRVLPVYFALSPRTPSNVNEVQLNYTLFPRDENDKSAPLTGKR"
BASE COUNT         1782 a         4463 c         4189 g         2213 t
ORIGIN      
        1 gcgtggagca gctccgcgcg caggagttcg tgacgccctc ggaagagccg gccgcgccct
       61 cccgccgcga attcctgaag ctcgccggcg ccggagcggc cttcctggcc gccgggtgcg
      121 cgcgcaagcc ggtggagaag atcctcccct acggcaaggc gcccgaggac atgcagccgg
      181 ggaaccccgt ctactacgcc tcgacgtgcg gcgagtgccc ggcggggtgc ggcctcctcg
      241 tgaagacccg cgaggggcgg ccgatcaagc tggagggcaa cccggaccac cccgcgaacc
      301 gtggcgcgct ctgcgcccgc gggcaggcgt cggtcatcaa cctctacgat cccgaccggc
      361 tgcggggtcc cgtcacggtg gatcgcgcca ccgggaagac gtcggcggcg ggctggggcc
      421 ccgtggacaa gcgcgtcacg atggcgctcc gcaaggcgcg cgacggcggc ggccgcgtgg
      481 tgctcctgac cggcaccgtc gccagcccca cgacgcgcgc cctgatccac gacttcgtcg
      541 ccgcgtttcc ggccgcggaa cacgtggcct acgacgcggt ctcgaacgac gcggccgcgc
      601 gggccggcga ggccacgtac ggcgatcgct cgctcccgcg ctaccgcctc gaccgggccg
      661 acgtgctcgt cacgctcggc gccgatccgc tcggcacctt cggcgacacg gtggagcagg
      721 gccgcgactt cgccaagcgg cgccgccccg agtcgggcgc gatgtcgcgc gtcatcgcgg
      781 tggagtcgat cctgaccttg accggcacga acgccgaccg ccgcgtgcgc gtccggcccg
      841 accagctcta tcccttcgcg atggcgctgg cctcgcgcct gggggccggg ggcgccgggt
      901 acacgcccga agccgtcgag gcggccgccg accttccgtc gggcaccatc gcctcgatcg
      961 ccgacgagct ggcccaggcg cgcggacgga gcctggtgat ggccgatccg aacgcggcgc
     1021 cggcggccca cgcggtggct ctgcagatcg ccgtcaacct gatcaacagc gcccttggaa
     1081 acgatggggt cacggtggac gcttcccgcg cctccggcgc ggtgcatggc tccggcgagg
     1141 agatgctccg gctggtcgag cggatgcgcg ccggcgagat ccaggcgctg atcgtctatg
     1201 ggacgaaccc ggcgtactcg cttccgaccg cggtgggctt cgccgacgcc ctgaagcgcg
     1261 tcccgttcgt ggcgagcatg gccgaccgcg ccgacgaaac cgcgcgcctc gccgacgtcg
     1321 tcgccccgag cacgcattac ctcgagtcgt ggggcgacca cgagccgcgc cagggcgtcc
     1381 tctccctgat gcagcccgca atcgcgccgc tctacgacgt gcgctccttc caggattcgc
     1441 tgctcgcctg ggcgcgctac ctggggcggg ggccgctcgc cgagaccgcg gggtcctacc
     1501 acgagtacct gcgcgagcgc tggcgcaccc aggtgtatcc cggcgcgggc gccgcggctc
     1561 ccttcgatct cttctgggag ggcgcgctgc gcaccggcgt gatcgcgcgc gaggagaacg
     1621 ccgatcgcac ggcatcgggg cgctcgccgc gggccggcgg cctgggcgga tcgctccccg
     1681 tcgcggcgac gccggagaac ggcgcgctga cgctggtcct ctatccctcg gccacgctgg
     1741 gtgacggacg gagcggcaac aacgcgtggc tccaggaact gcccgatccg gtctccaaga
     1801 tctgctggga caacttcgcc gcgatctcgc cgcaccgcgc ccgccagctc ggcgtgcgcg
     1861 actacgagat gaaggcggac gtggtcaccg tggacgtggg ccacgggcgg ttcgacatcc
     1921 cggtgcacgt gcagccgggc ctgcacgacg acgtcgtcgc gatcatgctc gggtacggcc
     1981 gcaccggcgc gggcacggtc gggaaccggg tggggcagaa cgcgttcgcc ctcgccgaag
     2041 cgacgggcgg ccaggtggga ctggcgggga tcccggtgcg cgtttcgcgc gctggaacgg
     2101 tcgtgccgat cgcctgcgtc caggggcatc agtacacgga agggcgcccg atcatctacg
     2161 agacgaccct gtccgagtat cagcgcgatc ccagcgcggg caacgaggga ggcgagctcc
     2221 taccctcgat gtggtcgcgg cacaagtatc cggggcacaa gtggggcatg gcgatcgacc
     2281 tgaacgcctg caccggctgc agcgcctgca tgctcgcctg catggtcgag aacaacgtcc
     2341 ccgcggtggg gaaggcgcag gtcctgcgcg ggcgcgaaat gcactggctg cggatcgacc
     2401 gctactactc cggcccggcc gaagatcccg agacggtgca ccagccgatg ctctgccagc
     2461 attgcgagaa cgccccctgc gagacggtct gcccggtgct ggccaccgtg cattccagcg
     2521 aggggctgaa cctccaggtc tacaaccggt gcgtcgggac gcggtactgc tccaacaact
     2581 gcccctacaa ggtgcgccgg ttcaactggt tcgactacac cgacaagccg aagtcgctcc
     2641 gcctggtctt gaaccccgac gtcacggtgc gcaccaaggg aatcatggag aagtgcacgt
     2701 tctgcgtgca gcggatccgc gacgctcgcg agcactcgaa ggttctcggc gtgccgatcc
     2761 aggaaggcga cctccagacc gcctgccagc agacctgtcc gtcgcaggcg atcaccttcg
     2821 gcgatcagaa cgatccgaag agccgggtgg cggacatggc tcgcaacaag cggagctatc
     2881 acgtcctcgc ggacctgaac acgctgccgt cgatcgccta catgaccaag gtccgaaacc
     2941 gcgaggagga gtccgcgtga acccgatggc cgtcggggaa gagcagcggg ccatggcctc
     3001 gcatgcgccg gccaccctac cgctggccga gccggtcctg atcaacccga ccaaggggtt
     3061 cgacgacgtc acgcgggaag tcgcgcgtcc catcggggag aagccgagca ggctgtggtg
     3121 gaccttcttc ggcatctcgg tcctcctcac cgcgcagttc gcggcggaga tcacctacca
     3181 gatcgtgaag ggaatcggga tctggggctt gaaccatccc gtcggatggg ccgtggacat
     3241 cacctccttc gtgttctgga tcggcatcgg gcacgccggc acgctgatct cggccattct
     3301 ccatctcttc cggcagcgct ggcggacggc gatcaaccgc tccgccgaag cgatgacgat
     3361 tttcgcggtg atgaccgcgg gactcttccc gttgatccac accggacggc cctggttcgc
     3421 gtactggctg atcccgtatc ccaattggcg gcatctctgg gtcaacttcc gctcgccgct
     3481 cgtatgggac gtgttcgccg tcagcaccta cctcacgatc tcgctctgct tctggtacac
     3541 ggggctgatc cccgacttcg cgacgctgcg cgaccggacc tcgggctgga agcgggggat
     3601 ctacagcctg ctctccctgg gctggcgcgg gtcggagcgg cactggcagc actacgagcg
     3661 cgcctacatg atgctggccg ggctgtcgac gccgctcgtg ctctcggtgc acagcgtcgt
     3721 ctccttcgat ttcgcgacct cgatcctgcc ggggtggcac acgacgatct tcccgcccta
     3781 cttcgtggcg ggggcgatct tctccgggtt cgcgatggtg gtgaccctgc tcgtcatcct
     3841 ccggaaggtg ttccatctcg aggagtacgt cacgctccac cacctcgaga acatgaacaa
     3901 gatcatcctg gcgaccgggt cgatggtggg ctacgcgtac gccacggagt tcttcgtggc
     3961 gatgtacagc ggcaaccagt tcgaatcgtt caccttcatg aaccgcgcga ccgggccctt
     4021 cgcctgggcc tactggacga tgatcacctg caacgtgatc attccgcagc tcttctggtt
     4081 ccggaagctg cggcgcagcc tgccggtcat gttcgtgatc tcgctcttcg tcaacgtggg
     4141 gatgtggttc gagcggttca acatcatcgt gacgtcgctc caccgcgact tcctgcccgt
     4201 caactggtcc tattacaagc ccacgatctt cgattactcg gtcatcgtcg gctcgttcgg
     4261 gttcttcttc atgtggttcc ttctcttctg ccgcttcttc ccgacgatct cgatcgccga
     4321 ggtgaagtcg gttctctgga gcgcgaagaa ggcgacgggg aacgtccccg ccgccgggca
     4381 cgcggagcgg acgcatggtt agcctcttcg gaaagcccaa gccgctgcac ggcgtgctgg
     4441 cgctgtacga caacccggac acgctcctga acgccgcggc ccaggtgcgg gagcacgggt
     4501 tcacgaaagc cgacgcctat accccctatc ccgtgcacgg cctgtccgag gccctcggca
     4561 tccggaagtc ctgggtgccg tacgtgaccc tggtgatggg gctgagcggt gccatcctgg
     4621 ggctggcgtt cgagatctgg acctcggccg tggactggcc gatcaacgtg ggcggcaagc
     4681 cgttcgtctc attgccggcg ttcatcccga tcacgttcga gtgcggcgtg ctgctgggcg
     4741 gcaccatgac gctggccgcg atgctcgtgg cgtgcggcct tcccaacttc cgcgacccga
     4801 tcctggaccg atccctgacc gacgaccggt tcgcgatctt catcgccgac tcggggcggg
     4861 gctggaacga gagccgcgcc atccagattc tccaggggag cggtgccgtg gacgtgcggg
     4921 tgatccgtgc gtagccgcgc gcgcggggcc gcggacgcga tggcggccgt cttggcggca
     4981 ggcgccctga tcgcttcggc gagcggttgc cagaagcggc agcccgcgct ggagtacatg
     5041 ccgaacatgg cctacggccc gcgcgtcgcc gcacaggccg aggacccgct ccgtcccggc
     5101 tattccgtgg agcggccgcc ggtggagggg accgtgccgc gtgacctcac cccctatcgc
     5161 ttcgcccagg ccgacacgac ccaggcgatg gcggcgctcg tcaatccctt gccgcgcacg
     5221 gccgaggtga tcggccgcgg ccagcgcgtc tacatgaaca cctgcgtcgt ctgccacggg
     5281 ccccgcggcg acgggcaggg gtacatcgag ccgctctatc cgcagccgcc gctcctcttc
     5341 tcggacaagg tgcggaactg gcccgacgcc cggatcttcc acatcatcac gcgggggcag
     5401 aatctgatgc cgagctacgc gagccagatc ctcccggagg atcgctgggc cgtggtgtac
     5461 tacgtgcgcg cgctgcagcg cgccgcgcgg cctctgcccc aggacctgcc cgcgaaggcc
     5521 cgcgccgccg gcgcggattc gacgggcgcg gccgccaaca cgaccggaag gagcgcttcc
     5581 cgatgaccgc cccggcctcc gcgcacgccg cgcccgtacg cgccgcggcg ccgctttcga
     5641 tcccgggcgg gatccgcgcc atgttcggcg ccttcatcct gatcggcatc gcgacgttcc
     5701 tgatcgagta ccacgggggc ggggatcaga cgcgcgccta cgcggcgttc ctgcttggat
     5761 acgtctactt cctgttcatc gcggtcgcgg gggtcttctt caccgcgctc cactacctgg
     5821 tcggcgctac ctggagcgtc gtcgtgcggc gcgtggccga ggcgttcacg tcctttctgc
     5881 cctacgcgct cctgctcttc gtggtgctgc tcgtcgggat cccgaagctc tacgtctggt
     5941 ccaacccgac cgtggccggg gaagtggcgg ccgagcacgt gaccaagggg ggctatctct
     6001 ccgtgccctt cttcacggcc cggaacctcg tcatcctcgc ggtgtggacg ctcttcggtt
     6061 ggtacttcgt gcggaactcc acgcggcagg acgcgacgcg cgatcccgtg ctgacgaaga
     6121 agagccttcg cgtggcgccg gccttcatcg tgatcttcgc catcacgctc acgctcctcg
     6181 gattcgatct gctcatgtcg ctcgagccgc tctggtacag caccatgttc ggcgtctact
     6241 gcttcgcggg cgcgtggcag agctgcctgg cgctcctgac gatcgcgatc gtgctcctcc
     6301 ggcgccaggg cgcgctccag ggcatcgtat cccggttcca ctatcacgac ctgggcaagt
     6361 acgtgttcgc gttctcgatc ttctggatgt acatcgcctt ctcgcagttc atgctgatct
     6421 ggtacgcgaa ccttcccgag gagacggagt ggatgatccc ccggacctat accgggtggg
     6481 ggatcatcgg gatcgcgctg atggtgtgca agttcgggat ccccttcttc gcgctgatgc
     6541 cggtgaaggt gaaggagagc gaggcgggac tcctcacggt ctgcgccatc gtgctggtgg
     6601 gacagtggct ggatctcttc tgggtgatcc tgcccgtgtt ctcccccgag cgcgtcgtgt
     6661 tcggctggac cgagatcggc gtcacgctcg gcttcctcgg gctcttcggc tggatcatcc
     6721 tcggattcct ctcgcggcac ccggtctcgc cgtcgggcga cccgcattac gaatcgagcg
     6781 tgaggttcca cggatgaccg agcacgccac cccgcaggaa ccggcccagg gatcggccgg
     6841 cagcgtcgtc ggcacctcgg agcacggctc cggagctggg cacggcccgc cgcggcacac
     6901 ggccgcgccc gaccgggaca agctctcctc cacggtcgcg gtcgcgcagt tcgtcttcac
     6961 gggcgtcatc ctcgcggtgg tggccgggct catgtacacg gccgggttcc actcgggaaa
     7021 gggcggcggc gagggcggcg ctccgggcgc cgggggcggt gccgcgccgg ccacgtcggt
     7081 ggacgtgcag gcgctcctca agcccacgcc cgagctggtg aaccagggaa agacgctgtt
     7141 ctcggtgaac tgcgcctcct gccacggcac gaacggcaag ggcgacggtc ccgcgtcctc
     7201 ggccctgaat cccaagccgc gcgatttcac gtccggctac tggcgctatg gcggtggcgt
     7261 cgcgcgcatc gtccagacca tcacgaacgg ctcgccgggc acggcgatgg cgcccttccc
     7321 gggcattccg atccaggacc gcttcgcgct cgcccagtac gtccgcacct tcaatccgtc
     7381 gaagcaggaa gccgacaagc ccgaggacct ggcctggctg gatcagttcg gcggcggcgc
     7441 gaagaaggac acgaccggag gcgccgccgt cgcggcgggt cctgcgcccg gggacaccat
     7501 cccgatcgcg cgcgccatgc agctgatcgc ggagcgcccc gccccggccc tcccggccat
     7561 ggccggcgcg cccgccgact ccagcgaggg cgggatgctc tacgccgcgc gctgcgcctc
     7621 ctgccacggc gcctgggggc agggcggcgt gcgcacgcgc atgatcggct cggcgcccta
     7681 cgtgtacgtc accacccgga gcctcgcggc cacggggccg ctggcggggg atgccgcggg
     7741 tttcgagcgg ctcatggtct acggtctcac gggcgggatg cacccctcgc acggggatct
     7801 cacgtcggag cagattcgcg tcctctacga ctacacccaa tcgttgcgcg cgcggctgtc
     7861 gagcgcggcg cgccccagga gttagaatga tctccatgcc gacgcgccgg acatggctcg
     7921 cgatcctggg ccttgcggtg ggactcctcg ccgcggggac ggccggagcc tggctcctcc
     7981 atcccgccac gcccgtcgcg cacgaagtgc ggaccctctg gtacaaggcc gtggcggtga
     8041 cctccttctt cctgatcctg gccgaggggc tgctcctgat cgtggtgctc cgcttccgcg
     8101 cgaagccggg ggtgaaggcc gcgaccttcc acgagaactt ccggctggag atgctctgga
     8161 ccgccattcc cgcgctcgcg ctggtctatc tcgcgggacc gtcgttctcg acgctcaagt
     8221 atctggagac cgttcccaag tcggacctga ccgtcgagat catcggacac cagtggttct
     8281 gggaatatcg ctacccgggg tacggcgtgg tcttcgccaa cgagccgctg gtcatcccgg
     8341 cggggaagat cgtggccgcc gacgtgacct ccatcgacgt cgtgcactcc tggttcctgc
     8401 ccgatttcgg cgtcaagatg gatgcgaacc ctggccgcgt gaaccacacc tggttccagg
     8461 tggccaagcc gggcacctac aacggacagt gcgccgagct gtgcggcgtc ctccatgcgg
     8521 agatgttcat cacggtgaac gtggtcccgc ccgaggaatt cgcccgctgg atcgagcaga
     8581 agaagagggg agcctagtcg catggaagca ggcacgctga cccggcccgt cgggaccgag
     8641 ctggtgcacg tcgggccgaa gccgcgcacc ggcatcatga agtacctcct gaccaccgat
     8701 cacaaactga tcggggtcat gtacatctgg ctctccttca cgttcttctt catcgccggc
     8761 atcttcgcga tggtcatgcg cgcccagctt tcgaaggccg gcctccacct gctccatccc
     8821 gagacgtaca accagatcat gtcgctccac ggcacgttca tggtcttctt ctggaccatc
     8881 ccggtgctgg cgggcatctc gaactacgtc gtgccgatcc agatcggcgc gcgcgacatg
     8941 gccttcccgc gcttgaacgc catctcgttc tggctcctga tccccgcggg cgggttcatg
     9001 ctcaccagcc ttctcctcca gggaggcgcc gcggcggccg gctggaccaa ttatccgccg
     9061 ctctccgacc ggcagttctc cccgggaccg ggcgtggatc tctggatcct ggggctccat
     9121 ctggcggggc tctcctccat cctcggcggc atcaacttcg tcgtgacgat cctgaacctc
     9181 cgcgcgcccg gcatgagcat catgaagatg ccgatgttct gctggaacgt gctgatcacg
     9241 caggcggtca tcctgatcgc gacccccgtg ctggcgggcg ggctcacgat gctcctcacc
     9301 gaccggctgt tcggcaccgg cttcttccac gcctcggcgg gcggcgatcc ggtcctctgg
     9361 cagcatctct tctggttcta ctcgcacccc gcggtctaca tcatggtcct gcccgcgatg
     9421 gggatggtgt cgcaggtgct gcccgccttc tccgacaagc ggatcttcgg ctacacgggg
     9481 atggtgatcg ccacggccgg gatcggcttc atcggcttcc tggtctgggg ccaccacatg
     9541 ttcgtgaccg gcatcgacca gcggctgcgc ttcttcttct cgttcgcctc gatggtcatc
     9601 gcggtgccca ccggcatcaa gatctggtcc tggctcgcga cggtctgggg cggcgcgatc
     9661 cgcttccgga cgccgatgct cttcggcctg ggcttcatct gcctcttcat catcggcggg
     9721 atcaccggcg tgtggctcgc gctcgtgccc ttcgacgtcc aggtgcacga cacctacttc
     9781 gtcgtcgcgc acctccatta cgtcctcttc ggcggctcga tcatggggat cttcgccggg
     9841 ttctattact ggttccccaa gatctcggga cgtctctaca acgaattctg gggcaaggtg
     9901 cacttctggc tggtgttcgt cggaatgaac gtggtgttca tgccgatgca cgtgatggga
     9961 atgctcggaa tgccccggcg catctacacc tacaagccgc agtacgaggg gctgaaccag
    10021 atctcgacca tcggcgccgg gatccttttc gtcgccatga gcatcttcct gatcaacgtc
    10081 ctcatgaccc tctggcgcgg caagcgcgcg ccggtcgacc cgtgggacca cgcggaatac
    10141 aacaagacgc tcgagtggac gatctcgtcg ccgccgcccg ccgagaactt cgcgcggatc
    10201 ccggtgatcc gataacagcg aggcgcccgt cagggcgccg aagctcatca tgagcgctgc
    10261 gaacgaccgc ctggttccgc tcctccgtct cacgctgctc ctggtgttcg tgctggtcgt
    10321 ctggggcggc atcgtccgac tctccgggtc ggggctggcc attcccgact ggccgctggc
    10381 gcacggcaag ctgatcccca agccgcagca caacgtcctg atcgaataca tccaccgggc
    10441 gatcgcgggc ctggtgagcc tgggaaccct cggcatcgcg ttcctggtct tccggagcgc
    10501 ccggtggcgc gcgcggctcc tgccgctcac tctcgccgcg ctgatcaccc tggcgctgca
    10561 ggtcttcatg ggtgcgcgcg tcgtgctgga agagctgccg gtggaccgcg tcgtggccca
    10621 tctcgtgatc gcgttcctct tcttcgccat cctgatccgg atggcgctgg tcgcgcgcga
    10681 cgccgaagac gaggcgggcg cctcgccggc ggccgccgcc ggggaagggg gggcggcgga
    10741 tccggggaag cggcggggga tcgcgctctg ggccaggatc gccgcggggg ccgtcctggc
    10801 ccaggtggcg ctggggggat gggtctcctc gtccggggcc gcgctcgcct gtcccgattt
    10861 tccgacctgc cagggaatgt ggctcccccc catgcacggg ctcctgggga tccactacgc
    10921 ccaccggctg ggcgcctacg ccgtcacgct gatcatcctg gcgatgctct tccgctcggc
    10981 cccgctggcg ctgccgccgc gcgcccggtg gccgctcagg atttcgggta tactcctcac
    11041 cattcagttt ctgctgggcg tggccaacgt gctcctccgc gtgcccctcc cggtcagcgc
    11101 gagccacctg ggcacggcgc tgatcctgtt cggaaccctc aacgtggcga gctacgagct
    11161 gtcccgtgcg tgattatatc gagctgacga agccccgcat cgtggccctg gtcatgatca
    11221 ccgccctggc cggcgtcgtg gcggagcgtt cgctgctttc ggacccgctc cggcttttgg
    11281 tcgtgctcgg cgccatcatg ctgaccgccg gctcggcgaa cgccttcaac cagtacttcg
    11341 aacgcgatct ggacgcgcag atggagcgca cccgggcgcg ccgcccgctg ccgcgccatc
    11401 gcgtggcgcc ggggcgcgcg ctggccttcg ccatcgcgat cggcgttctc tccgtcgtcg
    11461 ccctggcgca gtacggtagc ctcctcgccg cctggctcgc gttcgggacg atcgccttct
    11521 acggcttctt ctacacgctc tggctgaagc cgcgcacggt gcacaacatc gtgatcggcg
    11581 gggcggcggg agccatgggc cccgtgatcg cctgggccgc cgcggcgggg cacctggcac
    11641 tcgcgccggt gctgatgttc ctggtgatct tcctctggac cccgccccac ttctgggcgc
    11701 tcgccatctg cgtgcaggac gactatcgcc gggtgagcat cccgatgctg ccgatcgtga
    11761 agggggaggc ggagacctac cggcagatcg agctctacac gctcgtgctc gtcctggtca
    11821 cgctgctcat gccgttcgcg aacgccggcg ggctggtgct cggcgtcacg gcgctgttcc
    11881 tgggtgcgtg gttcgtggtg aaggcggttc gcgcccggcg cgcgcccacc gtggcggcgg
    11941 cgcgcggcgt cttcgcctac tccatcgtgt atctgttcgt gctcttcctg ggaatgatcg
    12001 ccgacgcgct ctggcgcatt ccgctccgct cctgacatgg aaccgatcgc cgacgccccc
    12061 gatcctccga tccggcgccg gattcccaat tgggtgatgg gcgtcgccct cgggaccgtg
    12121 gtgctcctga tgttctcctt cgcctacgcg aacgtcccgc tcttcaagct gttctgcgcg
    12181 cgattcggga tcggcgggtc ggggaaggcg gcgttccagt cggtctacgg cgaggggccg
    12241 accctttctt cgtcgacggg gccgatccag agccgggata tccaggtgaa gttcatgggg
    12301 gtctcggcga cggggctgcc ggtccggttc ggaccgtcgg tgccgctcgt gcacgtgatc
    12361 cccggacgcc cggtgcatct ggactacacg ttcacgaaca tgagcgacga ctcggtcttc
    12421 ttccgggccg tgcactcgat cgcgccgatc gaggccgcgc gcgagttcca gctcatccag
    12481 tgcttctgct tcgaagacca ggcgctgggc ccgcgcgaga cgcgggtgct gccggtctac
    12541 ttcgccctct cgccgcgaac gccgagcaac gtgaacgagg tgcagctcaa ctacaccctc
    12601 ttcccgcgcg acgaaaacga caaatccgcc ccgctcaccg ggaagcg
//
LOCUS       DASSLZ010000208         6095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_332889, whole genome shotgun sequence.
ACCESSION   DASSLZ010000208 DASSLZ010000000
VERSION     DASSLZ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332889"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2341
                     /locus_tag="VFM00_07985"
     CDS             <1..2341
                     /locus_tag="VFM00_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET7498227.1"
                     /translation="AEAILGAAALVAAGAALAAEVDAASPPLPVHPRAARVRVVGRVL
                     DTTTLEANPASVLFEARRVAVGGHEAACDARVVLRFGDEAGVPRWAFPGLWLEASGDF
                     RPPEDARNPGVEAPGRWLERSGVAGTIDADPLGVAAASDPSDPGAAETWVWRDRIARA
                     FDRALTAPVAGLARGMILGDRSLIAPATRNDFRDGGTIHILSISGLHVCILGGFVALG
                     AAALRLALVPALALELLAVWGYTFLVGAPASAARAALLWTAVRGGRAAGREVRPIAAW
                     GSAGLLLHLFDPRSVHDPGFQLSFAAVLGLLAAGGLGGESDEPDAAPVRVVKRWVRGA
                     AAIALQSAGATAGTTGLSARLFGALPVAGFVLNLAVVPLCGVFMGEAFFYLAADATRI
                     PFVRDAAAGALDASGLLLLAVNAWGARAIHPWPLRRAPTVAALAASAALLVVGAALRE
                     GSRHESPRAARALRRVALVATVAAGILPLAASFLPGVRRAAPAVLVAIDVGQGDALFA
                     RGSEGTTLLVDAGPATESRDMGKAAVEPLLRAEGLARLDRALLSHAHGDHFGGLEWLA
                     RRGWIPSLLENGSDPRAAWRRAIDADLRARGGRRFVVTRDTVIVETPTETLSIWAPPA
                     DSLVRARGNAIENNRSLVATLTLDGARTFLPGDAEDEAERADLARVAPVEILKAPHHG
                     SRTSSTAEWVRALAPRIVLVSCGEKNRFGHPSRATMGRYRLAAARVFRTDQEGAIRVT
                     IARGGAWVSTRAHPEPEWVSWRRPRSEGLAPKHSTGAEN"
     gene            2758..3318
                     /gene="purN"
                     /locus_tag="VFM00_07990"
                     /pseudo
     CDS             2758..3318
                     /gene="purN"
                     /locus_tag="VFM00_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
     gene            3355..4257
                     /locus_tag="VFM00_07995"
     CDS             3355..4257
                     /locus_tag="VFM00_07995"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET7498228.1"
                     /translation="MKATERAQRDVVVPGRAPDYRGKVRDIYDLGDRLLLVATDRLSA
                     YDVILNEGIPGKGRVLTQISRFWFETLRSIVPNHYVTTDVGRFPKPFSEHPELLEGRS
                     MLGLKAQRWDVECVVRGYLAGSGWKEYQQSGEVCGVKLPPGLRLSSKLPEPIFTPATK
                     EESGHDENITFDRMASIVGGETAERLRDVSIAIYKAAAAHAADRGLILADTKFEFGER
                     DGEPLWIDEALSPDSSRYWSIATYEEGVAQDSFDKQVIRDYLDSVKWDRTPPPPALPE
                     EVVEKAARRYEEALTLITGGRAAR"
     gene            4254..5921
                     /gene="purH"
                     /locus_tag="VFM00_08000"
     CDS             4254..5921
                     /gene="purH"
                     /locus_tag="VFM00_08000"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET7498229.1"
                     /translation="MNAIRISRALISVSDKTGLLELAHGLGRHGVKLLSTGGTATALR
                     DAGFGVRDVSEVTGFPELFGGRVKTLHPAVHGGILYRRGHTTDEEERHRHGIVSIDLV
                     VVNLYPFESTVARPGVRDEEAVEKIDIGGPALIRAAAKNHRHVVVVVEPAQYGAVIDA
                     LDQNEGAVPEALAREFAGRAYARTAEYDASIARYLSSRAGVPAAAGAGEARSATGVPG
                     FAVPSNGQGSEASGGERAGTLPDRWAGAYRKRQTLRYGENPGQEGALYAAEAGGGAVE
                     RLTQVRGKTLSYNNLLDIEAAVSLLREFGGRGGAVVVKHRNPSGAALAESAADAIAAA
                     RDGDSLSAFGGILGLNRPLDDAALDVVGKFFFEVIATPEIRASEARMAELRKNLVVLE
                     LGALLGQPAPAVVVRGLLDGLLAETALPAPRFDQWKSAAKRAPTEAETRDLRFAWSVT
                     RHVVSNAIVIAKGERTLGIGAGQSSRVDAVRLALWKAERSGHDVRGAVLCSDAFFPFP
                     DSVELAAKAGIAAVAHPGGSVRDGESTAAADAAGMALVLTGERCFLH"
BASE COUNT          807 a         2115 c         2263 g          910 t
ORIGIN      
        1 cgccgaagcg atcctcggcg ccgccgccct cgtggcggcc ggtgcggcgc tcgccgcgga
       61 agtggacgcc gcctcgccgc cgcttccggt ccatccgcgc gccgcgcgcg tgcgcgtcgt
      121 gggacgggtc ctcgacacga cgacgctcga agcgaatccg gcgtccgttc tcttcgaggc
      181 gcgccgggtc gcggtgggcg ggcacgaggc cgcctgcgac gcgcgcgtgg tgctgcgctt
      241 cggggacgag gccggcgtcc cgcgctgggc gtttccggga ctctggctcg aggcctcggg
      301 ggatttccgc cctcccgagg acgcgcgcaa tccgggcgtg gaggcgccgg ggcgctggct
      361 ggagcgctcg ggcgtggcgg ggacgatcga cgccgatccc ctcggcgtcg cggccgcttc
      421 cgatcccagc gatcccggcg ctgcggagac gtgggtctgg cgcgaccgga tcgcacgcgc
      481 gttcgaccgg gcgctgaccg cgcccgtcgc ggggctggcg cgcggcatga tcctgggcga
      541 ccgctccctg atcgcgcccg cgacgcggaa cgacttccgc gacggcggca cgattcacat
      601 cctctcgatc tcgggcctcc acgtgtgcat tctgggcggc ttcgtcgcgc tcggcgccgc
      661 ggcgctgcgg ctcgcgctcg tgccggcgct cgcgctggag cttctcgcgg tctggggcta
      721 cacgttcctg gtcggcgccc ccgcgtcggc ggcgcgcgcg gcgctcctct ggactgcggt
      781 tcggggcgga cgcgcggcgg ggcgcgaggt gcgtccgatc gccgcctggg gatcggccgg
      841 tctcctcctt catctcttcg atccgcggag cgttcacgac ccgggcttcc agctttcctt
      901 cgcggcggtg ctcgggctgc tcgcggcggg gggcctgggc ggcgagagcg acgagccgga
      961 cgcggctccc gtccgggtcg tgaagaggtg ggttcgaggc gcggccgcga tcgcgctcca
     1021 gagcgcgggc gcgacggccg gcacgacggg gctttccgcg cggctcttcg gcgcgctgcc
     1081 cgtcgcggga ttcgtgctga atctggccgt ggtgccgctg tgcggcgtct tcatgggcga
     1141 ggcgttcttc tacctggctg ccgacgcgac ccggatccca tttgttcggg acgccgcggc
     1201 cggagcgctg gacgcctcgg ggctcctcct tctcgcggtg aacgcctggg gggcgcgcgc
     1261 gatccatccg tggccgctcc gccgcgcgcc cacggtggcg gcgctggccg cgtcggccgc
     1321 gctgctcgtc gtgggcgccg ccctgcgcga gggctcgcgg cacgaatcgc cgcgcgccgc
     1381 gcgcgccctt cggcgtgtcg cgctcgtcgc gacggtcgcg gcggggatcc tcccgctcgc
     1441 cgcttcgttc ctgcccggcg tccggcgggc tgccccggca gttctcgtcg cgatcgacgt
     1501 ggggcagggg gacgcgctct tcgcgcgcgg ctcggaggga acgacgctcc tcgtggacgc
     1561 ggggcccgcg accgagtcgc gcgacatggg gaaggccgcc gtggagccgc tgctgcgcgc
     1621 ggaggggctc gcgcggctcg accgggcgct gctgtcccac gcccacggcg accacttcgg
     1681 cggcctggaa tggttagccc gccgcggctg gatcccttcg ctcctcgaga acggcagcga
     1741 tccgcgtgcg gcgtggcgcc gcgcgatcga cgccgatctc cgggctcgcg gcgggcggcg
     1801 cttcgtcgtc acgcgggata cggtgatcgt tgagacgccg acggagacgc tatccatctg
     1861 ggcgcctccc gctgactcgc tcgttcgcgc gcgggggaac gcgatcgaga acaatcgctc
     1921 gctcgtggcg acgctcacgc tggatggcgc gcggacgttc ctgccgggag acgccgaaga
     1981 cgaagcggag agggcggatc tcgcgcgcgt ggctcccgtc gagatcctca aggcgccgca
     2041 ccatgggagt cgcacctcca gcacagcgga gtgggtgcga gcgctcgcgc cgcgcatcgt
     2101 gcttgtctcg tgcggcgaga agaaccggtt cggccatcct tcgcgcgcga cgatggggcg
     2161 ttatcgattg gcggcggctc gcgtcttccg gaccgaccag gagggagcga tccgggtcac
     2221 gatcgcgcgc ggcggcgcct gggtctcgac ccgcgcgcat cccgagccgg aatgggtctc
     2281 gtggcggcgt cccaggtctg agggtctggc gccgaaacac tctacgggtg ccgagaacta
     2341 gaaggtaccg aagcggggcc gcggctcgat tgaagagcta gcgtgggggc ccgctgcatt
     2401 tgcggccggc gtttcttgga gcgcgtcaag cgtcgcggct ctcactttcg ccagcggcgg
     2461 tgaaatgaac gtcagttgtc gcggctccca cacgcgccag ccagggtgaa atgcatgaca
     2521 ggtgtcgctg atttcaccgc cgccagcggc ggtgaaatcg cattacagtt gtcgctggct
     2581 gatgatccct gcttgcccgc acggccggca tgccaaggcc ctatggtgcg gagaagagtc
     2641 ggtaggacaa gttacatata cattaacatg tgcggaaaca cgtccttgtg ccgtcttgac
     2701 tcgagaggcg ggcgtcgcct gtttgcgccc cttcgtcgcg tatgatagaa gtcgccgatg
     2761 aagccgaccc ggctcctgat cctggcctcg ggccggggga gccacgccgt aaacctcatc
     2821 cgggcgaccc gggatggccg gatcgagggg gaggtggtcc gcgtcgtgtc ggaccggtgg
     2881 gacgcggcgg cgatccgcga ggcggcggcg ctcggggttc cgacctcggt tctggagccg
     2941 gaacggccgg gcgcccggct cgcacccgag gcggaggcgg ggctgctcgc gatcgtgagg
     3001 gcggagcggc ccgatctgat cgcgctctgc ggattcatgc gcctgctccg ggcttcgttc
     3061 ctcgagcagg cgggcgtgcc ggtggtgaac gtgcatccgt cgctgctgcc cgcgttccgc
     3121 ggcctcgagc cgcagcgccg cgcgatcgag gccggcgcga cggtcaccgg cgccacggtg
     3181 cactacgtcg acgcgggcat ggacaccgga gcgatcgtgg cgcagaagga gctgccgatc
     3241 cggccggggg agacccccga ggagctggcg gcgcgcctcc tgccgctcga gcacgatctg
     3301 tacgtcgcgg ccattcgcag catccaacga accagcgaat ccaggagaga ccgcatgaaa
     3361 gccaccgaac gcgcgcagcg ggacgtcgtc gtccccggcc gcgctcccga ttaccgcggc
     3421 aaggtccgcg acatctacga cctcggcgac cggctgctgc tcgtcgccac cgaccgcctc
     3481 tcggcctacg acgtgatcct gaacgagggg atcccgggca aggggcgcgt gctgacgcag
     3541 atctcgcgct tctggttcga gacgctccgc tcgatcgtgc ccaaccacta cgtcaccacc
     3601 gacgtgggcc gctttcccaa gcccttctcc gaacatccgg agctcctcga aggccgctcg
     3661 atgctcggac tcaaggcgca gcgctgggac gtggagtgcg tggtacgggg atacctggcg
     3721 ggatcgggat ggaaggagta ccagcagtcg ggcgaggtct gcggcgtgaa gcttccgccg
     3781 ggactgcgcc tctcctcgaa gctgcccgag ccgatcttca cgccggccac caaggaggaa
     3841 tcggggcacg acgagaacat cacgttcgac cgcatggcct cgatcgtggg cggtgagacg
     3901 gccgagcgcc tgcgcgacgt ctcgatcgcc atctacaagg cggccgcggc gcacgcggcc
     3961 gaccgcgggc tgatcctggc cgacaccaag ttcgagttcg gcgagcggga cggcgagccg
     4021 ctctggatcg acgaggcgct ctcgcccgac tcctcgcgct actggtcgat cgcgacctac
     4081 gaagagggcg tggcgcagga ctccttcgac aagcaggtga tccgcgacta cctcgactcg
     4141 gtgaagtggg atcgcacgcc gccgcccccg gcgctgccgg aggaggtggt cgagaaagcg
     4201 gcccgccgtt acgaggaagc gctcacgctg atcaccggag gacgggcggc gcggtgaacg
     4261 ccattcggat ctcccgcgcg ctgatctcgg tctccgacaa gaccggcctc ctggagctgg
     4321 cgcacggtct ggggcggcat ggggtgaagc tcctgtcgac cggagggacc gcgaccgcgc
     4381 tgcgcgacgc cggcttcggc gtgcgcgacg tgtccgaggt gacgggattt ccggagctct
     4441 tcggcgggcg cgtcaagacg ctccacccgg cggtgcacgg gggaatcctc taccggcgcg
     4501 ggcatacgac ggacgaggag gagcgccacc ggcacggtat cgtctccatc gatctcgtcg
     4561 tcgtgaatct ctatcccttc gagtccaccg tcgcgcggcc cggcgtgcgc gacgaggagg
     4621 ccgtggagaa gatcgacatc ggcggcccgg cgttgattcg cgccgcggcc aagaaccatc
     4681 gccacgtcgt cgtggtggtc gagcccgcgc agtacggcgc cgtgatcgac gcgctcgacc
     4741 agaatgaggg ggccgtgccg gaggcgctcg cgcgcgagtt cgcggggcgt gcctacgcgc
     4801 gaaccgccga gtacgacgcg tcgatcgcgc gctacctgtc gtcgcgcgcg ggcgtgccgg
     4861 ccgcggcggg agcgggggag gcgcgctcgg ccaccggcgt gccgggcttc gccgtgccgt
     4921 cgaacgggca gggctccgag gcgagcggcg gcgagcgtgc agggactctc ccggatcgtt
     4981 gggccggcgc ctatcgcaag cgtcagaccc tacgctacgg cgagaacccc ggacaggaag
     5041 gggccctcta cgccgcggaa gcgggaggcg gcgcggtgga gcggctgacg caagtccgcg
     5101 gaaagacgct ctcctacaac aacctcctcg acatcgaggc ggcggtgtcg ctgctgcgcg
     5161 agttcggcgg gcggggcgga gccgtcgtcg tgaagcaccg gaacccctcg ggcgccgcgc
     5221 tggccgagag cgcggccgac gcgatcgcgg cggcgcgcga cggcgactcg ctctcggcgt
     5281 tcggcgggat cctgggactg aaccgcccgc tcgacgacgc cgcgctcgac gtggtgggga
     5341 agttcttctt cgaggtgatc gccacccccg aaatccgggc gagcgaggcg cggatggcgg
     5401 agcttcgaaa gaatctcgtc gtgctcgagc taggtgcctt gctgggccag cccgcgccgg
     5461 ccgtggtggt ccggggactc ctcgacgggc tcctggccga gaccgcgctc cctgcgcccc
     5521 gcttcgacca gtggaagtcc gccgcaaagc gcgcaccgac cgaggccgag acgcgcgacc
     5581 tccgcttcgc gtggagcgtg acgcgccacg tggtctcgaa cgcgatcgta atcgccaaag
     5641 gggagcgcac gctcgggatc ggggcggggc agtcctcgcg cgtggacgcc gtgcggctcg
     5701 cgctgtggaa ggcggagcga tcgggccacg acgtgcgcgg cgccgtgctc tgctccgacg
     5761 ccttcttccc gttcccggac tcggtggagc tcgccgccaa ggcgggaatc gccgcggtgg
     5821 ctcatcccgg cggctcggtg cgcgacggcg aaagcaccgc ggcggcggac gcggcgggca
     5881 tggcgctggt cctcaccggc gagcggtgct tcctgcactg atctgcggta ttctcacgga
     5941 atcccgtccg tgcactgaac tcgtaccgag gaggaagacc ccctgaatga tcccaccgct
     6001 tccaaccttc tcgagatgaa ccccgccacg cagaaaccgg ccccgcgcac gacccacgaa
     6061 cgcctcgtca tcctcgactt cggctcccag ttcac
//
LOCUS       DASSLZ010000209        13022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_459293, whole genome shotgun sequence.
ACCESSION   DASSLZ010000209 DASSLZ010000000
VERSION     DASSLZ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13022
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459293"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFM00_08005"
     CDS             <1..622
                     /locus_tag="VFM00_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET7498230.1"
                     /translation="RIERFLQLAARDNIQVAYPTTPAQYFHLLRRQVKRPWRKPLIVM
                     TPKSLLRHPDAVSPMTELATGKFERALPDAAVPAAKAKRVLLCSGKIYYELLRTREDL
                     GEKTTAILRLEQLYPLAEETLELLFAGLPKDLAITWVQEEPRNMGAWPFLRARFGDRL
                     LGRHPFHVVSRAESATPASGAASSHKIEQERLLLAAFDKEPSAHGG"
     gene            612..1958
                     /gene="odhB"
                     /locus_tag="VFM00_08010"
     CDS             612..1958
                     /gene="odhB"
                     /locus_tag="VFM00_08010"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET7498231.1"
                     /translation="MAVELRVPEVGESIKEVQIGAWLKGEGDRVERDEAVVEIESDKA
                     TLELPAPVSGTIEKLLKKQGDVVKIGELIATVTEGASGNGAKPKKAAGPAPAAKSAAA
                     AQPAGTAQPATPTPAPAPPPAQAKPASKATPAPGATPMPAPPSAAPPRMPSAERLMGQ
                     HGIAPSDVPASGPGGRVLKEDVLEHMARKPEPGRPRTEPGAPAEAAPIEAAPGEPSAT
                     EEAVPMSPLRLRVAERLKEAQNTAALLTTFNEIDMSGVVALREELQQPFQERYGVKLG
                     FMSFFVKAAIDALMRFPQVNAEIRDKEIIYKNYYDIGIAVGGGRGLVVPVLRGADRLS
                     FAEIEIAIADFAQRARDNKLSLDELKGGTFTISNGGVYGSLLSTPIVNPPQSGILGLH
                     AIHKRPVAIDDEVVIRPMMYVALTYDHRIIDGRESVTFLRHVKELIERPTRMLLGL"
     gene            1972..3363
                     /gene="lpdA"
                     /locus_tag="VFM00_08015"
     CDS             1972..3363
                     /gene="lpdA"
                     /locus_tag="VFM00_08015"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7498232.1"
                     /translation="MTNAKHDLVVIGSGPGGYVAAIRAAQLGLNTACVEFEPALGGTC
                     LRVGCIPSKAMLESSERYHETKEALAPHGIQVQGVSFDLGAMMKRKDDIVKGLTGGVD
                     MLFKKNKVTRYSGRGKITGPGRVSVEGGAEPAMLEAKNILIATGSKSAPLRGVTVDGD
                     RIGTSTEALSWPEVPARLAVIGAGYIGLEMGSVWLRLGSKVTVLEYLDRILPGMDAEI
                     ATEAFRLFKRQGMEFRLSSKVTGAKVEGKEVVVTCEGQEPLRVDRVLLAVGRIPYTEG
                     LGLESVGVKPDDRGRLPVDEHYRTSAEGVYAIGDVIGGAMLAHKASEEGVACVEWIAT
                     GYGHVNYEAIPGVVYTQPEIASVGRTEEELTAAGVAFKKGVFFFRGIGRSRALGQIDG
                     RVKMLADAKTDRILGVHIIGPRAGDLIAEMVAAIEFSASSEDIARICHAHPTLAEAVK
                     EAALAVDGRPIHA"
     gene            complement(3427..3873)
                     /locus_tag="VFM00_08020"
     CDS             complement(3427..3873)
                     /locus_tag="VFM00_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498233.1"
                     /translation="MFHFGPLQFLLYLALVPLALIPSIFYALTLHRALARCAPESRAM
                     EPGMVWLMFIPLFNLFWNFVVVNRVSDSLEREFHRRGAAGPVDTGRSVGIATCALICV
                     SLIPLLGILTSFIALVCWILYWAKVANLSGQLLPPLGSAQPSAPVA"
     gene            complement(4264..7044)
                     /locus_tag="VFM00_08025"
     CDS             complement(4264..7044)
                     /locus_tag="VFM00_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7498234.1"
                     /translation="MPAFAATTSSGPTRPSDRGVIREEYPVPTTRPSTTVPLSKKLPL
                     RTPPPLEETLVFQDSLNALSIGNQGNWTHVDNSAKPTAWHIDTFYGCQGNAWWCGRID
                     STWIYDTNRGGYENSWTQYLQNGVWLDSLPNNQLIKLSFRHHFNAEQGFDFGKVEVFD
                     SSQNDWLTLASYTGKVPTNGTCDTTTVIIPDSIATVFYSTVDRSQHQLVQFRFSFTSD
                     IAYSSQDGLYDGDGWSIDNITVKVGTSLRFFDNCEGGQGTWAPSIFPPVGDLYAISNN
                     VFTEDICTTNRTNVWSDWDAATLSVVPRQDNLLRTPSVFINKPGEGFVLFDVYRNLPA
                     AACFYYHLRYRTRNAGEPVWTGWVDPTRVLYYGASKDWARQKVVLPGAANRDSLQVEF
                     GLTDYGSIYCDGAGNGNGVYTFFDNVAVGVVTVAPPIFIQRDIDLFNDTFSTTAFFKD
                     DNFNTALGDSAVVEVSTSRGYKSGFMYYRFNGGSWSSTPLGKAAAALPNLRYADVPAV
                     AYPANTNLEYYFGVTDSTDATTYLPAGAITDGTYFSAAILPLKTATNPANACFDSLAT
                     VLFINNYSGREPKNLWADALKAQGYKFDTWDVNAPTSALGNTPGGAATSDPYDWPPTP
                     VSALTQYSTIIWHAGSLTAFTLSQQDQALFQSWIQQSGKSRNLWIAGDNIAYDLTVNS
                     RDYNSFMSFTCGMKYLRDMWENAPQDTLHPLVQGFAGSPTAGRFMHVNDDCPILDKND
                     LIATSTQGAANGKVGVALKYPNNFAAATRYATKYVSFGSDSARVLFQGFNFSNIEEGG
                     ERLNYAKNIMQGYFGVPNCYSPTGVDEEPGAGAPSIRNTLGQNAPNPFNPSTVIRYTI
                     AETGPVTIRIFNAGGALVRTLVDGQHAPGEYTVRWDGKDANGQRLSSGVYFYRIETGA
                     GFRDSKKLILLK"
     gene            complement(7252..9534)
                     /locus_tag="VFM00_08030"
     CDS             complement(7252..9534)
                     /locus_tag="VFM00_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7498235.1"
                     /translation="MIVFLIVLALLVLILVVPPIRRNLITRPLMPLVAGALPRMGDTE
                     RIALEAGSVWWEAELFSGRPEWKRLLAFRAKALTEEERAFLAGPVEELCRMLNEWEVM
                     KGHDLPPEVWSFLKKHRFFGMIVPKEYDGLGFSANAHSAVITKISSRSVTASVTAMVP
                     NSLGPAELLLHYGTEEQKRHYLPRLARGEDIPCFALTEPEAGSDATSQKSVGIVTRGI
                     YQGREVLGMRLNWSKRYITLSPVATVIGLAFVLRDPDRLLGDKEEIGITCALLPANLP
                     GIMIGPRHDPLGVPFLNGPTEGHDVFAPLDAIIGGSANAGIGWRMLMETLASGRGISL
                     PSLAVGCAQLATRTVTAYANVREQFGLPIGRFEGIEEPIARIGGRTYLMDAARRLSLG
                     AIDEGQKPAVISAIVKRYLTEGMRAVVNDAMDVQAGAAISRGPRNVMAPAYAAVPVGI
                     TVEGANILTRSLIIYGQGAIRCHPYVQREMAAIARRDLAAFDRAFFGHLGHVVSTVGR
                     AFLDGLTGGAFEGVPVSGPARPLFGRLSHLSAAFALTSEAAMATLGGTLKRRERLSGR
                     LADALAYLYLSSAALKRFVDEGQLRRDLPLVRWSCAFAFHEIETAISGVLDNMPSRPT
                     AWLVRLAAFPLGFRAKPPADRLSAAVARGLLDDRALRLAMTSDVYIPPASEGSLGRLE
                     AAYAKVVAAQPIDRKLKDAVRTRRIPAHLDAEAQLAAAVTAGVIDEDERRIWREADVA
                     RRDAIQVDAFPLEEFAELAV"
     gene            9637..10737
                     /locus_tag="VFM00_08035"
     CDS             9637..10737
                     /locus_tag="VFM00_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET7498236.1"
                     /translation="MAPFRSFSIAEDIRVASTLPAWVYSDPGVFADCRERVFVPSWQL
                     AGDTDRVKIPGQVCPVPLLEGMIDEPVLLTRDAEDRVHCLSNVCTHRGNLVVESEGVE
                     HVLRCRYHGRRFALDGRFHSMPEFDRTEGFPSAADDLPRVPFGTWGKFLFASLRPAVP
                     LETLVGPMDARCGWLPIAEAALDPSRTREYIVRANWALYCDNYLEGFHIPYVHAALSD
                     ALDYGQYTTETHGRSVLQVGVAKEGEDAFDLPRSSPDHGRRIAAYYWWLFPNLMFNFY
                     PWGISVNVVRPLDVDRTKVLFLAYVWDPSRLDRGAGAALDRVEREDEVIVESVQRGVR
                     SRLYERGRYSPTREQGVHRFHQLLAEVLGSER"
     gene            10913..>13022
                     /locus_tag="VFM00_08040"
     CDS             10913..>13022
                     /locus_tag="VFM00_08040"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HET7498237.1"
                     /translation="MSRAGARTIPACVMLALSLVLALLALAAPRAEALPGFSPTDEGP
                     SYADSADGRKSASPTIQAYRLDNQEIRLDGHLDDAAWQAAPAGRGFTVWEPDRGVTPA
                     EQTVFKVAYDEGAVYFGIACLKSDPKAKIAATLTRRDHITNSDIVAIYLDPYHDHTSG
                     YAFKVNPLGVQLDSYIYNNGDGNDDDWDAVWEAETSRDDQGWYAEIRIPFSAIRYRSA
                     EDMTWGLNVWRYMKGLGQDTAWHTWDRKQAGFVSQFGQLTGLRGIRAPRQIEVLPYVV
                     QRETDHAMISSKTRPDRLDGFQDAGADFKYGVTSDLTLNATVQPDFGQVEADPAVLNL
                     SPFETSYQEKRPFFVEGSRFFQHPTFNLFYSRRIGTGDENARIRYAGKLTGKTVGGIS
                     VAALAASTDLTHDGQAHNIFKSGDRLSRFFVGRFGKEFAQGRHQINFMQTGVLNTADR
                     STWGDRASREAYTSGLDFALTSLHRSWRASGSFVGSIVNPEPAPGVSDAPRYGTGGTF
                     QVDRMGGKWRGNFGGRWESGKLDINDMGYLQAPDEIDSYAWAQRRFNPEGKSRVYNSA
                     NLNFNFNRSWMYAGRTGYDVGTGLPAWHYEPGHPQYLTSNINGWMQFKNFREAWFGML
                     YNDWGHHRYETRGGPIISEPLTYGGWVGASTDRRKNLNASSEVSWNKDVAQNVSLDLT
                     TDINWNQSSAVNHTLSLGYHN"
BASE COUNT         2213 a         4325 c         4430 g         2054 t
ORIGIN      
        1 ccggatcgag cgcttcctcc agctcgccgc gcgggacaac atccaggttg cgtatccgac
       61 cacccccgcg cagtattttc acctgctgcg ccgccaggtg aagcggccgt ggcggaagcc
      121 gctgatcgtg atgacgccga agagcctgct ccggcatccc gacgcggtgt cgccgatgac
      181 ggagctcgcg accgggaagt tcgagcgcgc gctgccggac gcggcggtgc cggcggcgaa
      241 agccaaacgc gtgctgttgt gctcggggaa gatctactac gagctcttgc ggacgcgcga
      301 ggatctgggc gagaagacga cggcgatcct ccggctggag cagctctacc cgctcgccga
      361 ggagacgctg gagcttctgt tcgcggggct ccccaaggat ctcgcgatca cctgggtgca
      421 ggaggagccg cgcaacatgg gcgcgtggcc gtttttgcgc gcgcggttcg gagaccggct
      481 gttgggccgg caccccttcc acgtcgtgag tcgcgcggaa tcggcgacgc ccgcaagtgg
      541 tgccgcgagc agccacaaga tcgagcagga gcggctcctg ctcgcggcct tcgacaagga
      601 gccttcggcg catggcggtt gagctcagag ttcccgaagt cggcgagtcc atcaaagagg
      661 tccagatcgg cgcctggctg aagggcgagg gcgatcgggt ggagcgcgac gaggcggtgg
      721 tcgagatcga gtccgacaag gcgacgctgg agctgcccgc gccggtgtcg ggcacgatcg
      781 agaagctgct caagaagcag ggcgacgtcg tgaagatcgg cgagctgatc gcgaccgtga
      841 cggaaggcgc gagcggaaac ggcgccaagc cgaagaaggc ggcagggccc gcgccagcgg
      901 ccaagagcgc ggcggcggct caacccgccg gaacggctca gcccgccacg ccaacaccgg
      961 ctccggcccc gccgccggcc caagccaagc ccgcctcgaa ggcaacgccc gcgccgggag
     1021 cgacgccgat gccggcaccg ccctccgccg cgcccccgcg catgccctcc gccgaacggc
     1081 tgatgggcca gcacggcatc gcgccgtccg acgtgcccgc gagcggtccg ggcggccgcg
     1141 tcctcaagga ggacgtgctg gagcacatgg cgcgtaagcc cgagccaggg cggccgcgca
     1201 ccgagcccgg cgctccggcc gaggcagcac cgatcgaggc ggcgccgggc gagccctccg
     1261 ccaccgagga agcggtgccg atgtcgccgc ttcgcctgcg cgtggccgaa cggctcaagg
     1321 aagcccagaa caccgccgcg ctcctcacca cgttcaacga gatcgacatg tcgggcgtgg
     1381 tggcgctgcg cgaggagctc cagcagccct tccaggaacg ctacggcgtg aagctcggct
     1441 tcatgtcgtt cttcgtaaag gcggccatcg acgccttgat gcgcttcccg caagtgaacg
     1501 ccgagatccg cgacaaggag atcatctaca agaactacta cgacatcggc atcgcggtgg
     1561 gcggcgggcg cgggctggtc gtgccggtgc tgcgcggggc cgaccgcctc tccttcgccg
     1621 agatcgagat cgcgatcgcc gacttcgccc agcgcgcccg ggacaacaag ctgagcctgg
     1681 acgagttgaa gggcggcacg ttcaccatct ccaacggcgg cgtctacggc tcgctcctgt
     1741 cgacgccgat cgtcaacccg ccccagagcg ggatcctcgg gctgcacgcc atccacaagc
     1801 gcccggtcgc gatcgacgac gaagtcgtca tccggcccat gatgtacgtc gccctcacct
     1861 acgatcaccg catcatcgac ggccgtgaat cggtcacttt cttgcgtcat gtgaaggagc
     1921 tgatcgagcg gcctacccgg atgctgctcg gactctgacc tcggaacgga aatgacaaac
     1981 gcaaagcacg acctggtcgt catcggatcc ggccccggcg gctacgtggc cgccatccgc
     2041 gcggcacagc tgggactgaa taccgcctgc gtggaattcg agcccgcgct ggggggcacc
     2101 tgcctccgcg tcggctgcat cccgagcaag gcgatgctcg aatcgagcga gcgctatcac
     2161 gagacgaagg aggcgctggc cccgcacggc atccaagtgc agggggtctc gttcgacctc
     2221 ggcgccatga tgaagcggaa ggacgacatc gtgaagggcc tgaccggcgg cgtggacatg
     2281 ctcttcaaga agaacaaggt cacgcgctac tccggccgcg gcaagatcac cgggccgggc
     2341 cgcgtctcgg tcgaaggcgg cgccgagccc gcgatgctcg aggcgaagaa catcctgatc
     2401 gcgaccggca gcaagtcggc cccgctccga ggcgtcaccg tggacggcga ccggatcggc
     2461 accagcaccg aggcgctctc ctggcccgag gtgccggcgc gcctggccgt gatcggagcg
     2521 ggctacatcg gtctcgagat ggggtcggtc tggctccgcc tgggctccaa ggtcaccgtg
     2581 ctggaatacc tcgaccgcat cctccccggc atggacgcgg agatcgcgac cgaggcgttc
     2641 cgcctgttca agcgccaggg gatggagttc cggctgagtt ccaaggtcac gggcgccaag
     2701 gtcgagggga aggaagtcgt cgtcacgtgc gaagggcagg agccgctccg cgtggaccgc
     2761 gtgctcctcg ccgtcggccg cattccgtac accgaggggc tggggctgga atcggtcggg
     2821 gtgaaaccgg acgaccgcgg ccggctcccc gtggacgagc actaccgcac gtccgccgag
     2881 ggcgtgtacg ccatcggcga cgtgatcggg ggcgcgatgc tcgcgcacaa agcttccgaa
     2941 gagggggtcg cgtgcgtcga atggatcgcg accggctacg gccacgtgaa ctacgaggcg
     3001 atcccggggg tggtctacac gcagcccgag atcgcgtcgg tcggccgcac cgaagaggag
     3061 ctgaccgcgg ccggcgtcgc cttcaagaaa ggggtcttct tcttccgcgg catcggccgc
     3121 tcgcgcgccc tcggccagat cgacggccgc gtgaagatgc tggcggacgc gaagaccgac
     3181 cgcatcctgg gcgtgcacat catcggcccc cgcgccggcg acctcatcgc cgagatggta
     3241 gcggccatcg aattcagcgc gagcagcgag gacatcgcgc ggatctgcca cgcgcatccc
     3301 acgctggcgg aggcggtgaa ggaagcggcg ctggcggtgg atggccgtcc catccacgcg
     3361 tagggttcgc ttccgaggca acttcgaaaa cgaccaacat gccccagggc atgttgccgt
     3421 ccgaagctac gcgacgggag ccgagggctg cgcggacccc aacggcggca gcaactgccc
     3481 ggacagattc gccaccttgg cccagtagag aatccagcag accagcgcga tgaacgacgt
     3541 gaggattccc agcagaggga tcaagctcac gcagatcagc gcgcaggtcg cgatccccac
     3601 gctgcgcccc gtgtccacgg gccccgctgc cccgcgacga tggaactccc gctccaggga
     3661 gtccgagacc cggttcacca cgacgaaatt ccagaacagg ttgaacaggg ggatgaacat
     3721 cagccagacc atcccgggct ccatcgcgcg actctcgggc gcgcagcggg cgagtgcgcg
     3781 atgcagggtg agggcgtaga agatcgacgg gatgagcgcg agcgggacga gcgccaggta
     3841 gagcaggaat tgcaatggcc cgaaatggaa catggtccct ccgagattag ggacacgaca
     3901 gcatcggctg acatgcttca aagcatgcaa gttaactcag atgttactgg cccgcctggg
     3961 caaagtatct tccgaagcgt tcctatgcca gattggcacg ttgaacgtac tctggagtat
     4021 gttatcgact aacgaccgat ccgtgctcaa ccacattgtt ccagaacatg ttcttgtgcc
     4081 gtcctgacac gctcctgaca tccggtgcgg gttcattcat acatcgattc acggccaaca
     4141 gggtcattcc aggaactgac cgcagggtct ggacgaggga cagaaggagc agccgaagcc
     4201 cggggaagcg acgagcagca cggcttcagc agaatcagct tcctcgggaa acgacgagct
     4261 ggactacttc agcaggatca gcttcttcga atcgcggaag cccgcgccgg tttcgatgcg
     4321 gtagaagtag acgccgctgc tcagacgctg gccgttggcg tccttgccgt cccagcgcac
     4381 ggtgtactcg cccggggcgt gctggccgtc caccagcgtg cgcaccaggg cgccgccggc
     4441 gttgaagatc cggatcgtga cggggccggt ctcggcgatc gtgtagcgga tgacggtgct
     4501 cggattgaac gggttcggcg cgttctggcc gagggtgttg cggatcgagg gcgcgccggc
     4561 gccaggctcc tcgtcgacgc ccgtcgggct gtagcagttg ggcaccccga agtagccctg
     4621 catgatgttc ttcgcgtagt tcaggcgctc cccgccctcc tcgatgttgc tgaaattgaa
     4681 cccctggaag agcacgcgcg cgctgtccga tccgaacgag acgtacttcg tcgcgtaccg
     4741 ggtcgcggcg gcgaagttgt tcgggtactt gagcgcgacg ccgactttcc cgttcgcggc
     4801 cccctgcgtc gaggtcgcga tgagatcgtt cttgtccagg atcggacagt cgtcgttcac
     4861 gtgcatgaag cgtccggccg tcggcgatcc ggcgaagccc tggaccagcg ggtgcagcgt
     4921 atcctggggc gcgttctccc acatgtcgcg caggtacttc atcccgcagg tgaacgacat
     4981 gaaggagttg taatccctgc tgttcaccgt caggtcgtag gcgatgttgt cgccggcgat
     5041 ccagagattt cggctcttgc ccgactgctg gatccaggat tggaacagcg cctgatcctg
     5101 ttgcgacagc gtgaacgcgg tcaggctgcc cgcgtgccag atgatggtgg agtactgcgt
     5161 cagagcgctc accggcgtcg gcggccagtc gtagggatcg gacgtggccg cgcctcccgg
     5221 ggtgttcccc aacgcgctgg tgggagcgtt cacgtcccag gtgtcgaact tgtatccctg
     5281 ggccttcagc gcgtcggccc acaggttctt gggctcgcgt ccggagtagt tgttgatgaa
     5341 gagcaccgtc gcgaggctgt caaagcaggc gttcgccgga ttggtcgccg tcttcagcgg
     5401 caggatcgcg gccgagaagt aggtgccgtc ggtgatcgct ccggcgggaa gatacgtcgt
     5461 cgcgtcggtg ctgtcggtga cgccgaagta gtactcgaga ttggtgttcg ccgggtaggc
     5521 gacggccgga acgtcggcgt agcgcagatt cgggagcgcc gcggccgctt tcccgagcgg
     5581 cgtggagctc cacgagccgc cgttgaaccg gtagtacatg aagcccgact tgtatcctcg
     5641 tgaagtgctc acttcgacca cggcgctgtc gcccagggcc gtgttgaagt tgtcgtcctt
     5701 gaagaaagcg gtcgtgctga aggtgtcgtt gaagaggtca atgtcgcgct ggatgaagat
     5761 gggcggcgcc acggtgacga cgccgaccgc gacgttgtcg aagaaggtgt agacgccgtt
     5821 cccattgccg gccccgtcac agtagatgct gccgtagtcg gtcagcccga actcgacctg
     5881 cagcgaatcc cggttcgccg ctcccgggag caccaccttc tgccgcgccc agtccttcga
     5941 cgcgccgtaa tacaggacgc gcgtcggatc cacccaccca gtccataccg gctcgccggc
     6001 attccgggtg cggtagcgca ggtggtagta gaagcatgcg gcggctggga ggttccggta
     6061 gacgtcgaag aggacgaatc cctcgccggg tttgttgatg aagacggacg gagttcggag
     6121 gaggttgtcc tggcgcggta ccaccgagag ggtggccgcg tcccaatccg accatacgtt
     6181 ggtgcggttg gtggtgcaga tatcctcggt gaacacgttg ttactgatcg cgtacaggtc
     6241 gcccacgggc gggaagatcg atggagccca cgtgccctgg ccgccctcgc agttgtcgaa
     6301 gaaccggagc gaggtgccta ccttgaccgt gatgttatcg atgctccagc cgtcgccgtc
     6361 atagaggccg tcctgggacg agtaggcaat gtcgctggtg aaggagaatc ggaactggac
     6421 caactggtgc tgcgaacggt ccacggtcga gtagaacacg gtggcgatcg agtccggaat
     6481 gatcacggtg gtcgtgtcgc aggttccgtt ggtgggcacc ttcccggtgt agctggcgag
     6541 cgtcagccag tcgttctgac tcgagtcgaa cacctcgacc ttcccgaagt cgaagccctg
     6601 ctcggcgttg aaatgatgtc ggaaggacag cttgatcaat tggttgttgg ggaggctgtc
     6661 gagccagacg ccgttctgca gatactgcgt ccagctgttc tcgtagccgc cccggttcgt
     6721 gtcatagatc caggtcgagt cgattcgccc gcaccaccag gcgttcccct ggcagccgta
     6781 gaaggtatcg atgtgccagg ccgtcggctt ggccgagttg tcgacgtggg tccagttccc
     6841 ctggttgccg atcgagagcg cgttcaggct gtcctggaat acgagcgtct cttccagggg
     6901 aggcggcgtg cggagcggca gcttcttgga gagcgggacc gtcgtggacg gccgcgtggt
     6961 gggaacgggg tactcttcgc gaatgacgcc gcgatcggac ggacgggtag gacccgacga
     7021 ggtcgtggca gcgaacgcgg gcacggcgga agccagtgcc acggcgaagc cgatcaggat
     7081 gcgtaaaacg cgcaggtgca agcggttcgc ctaaggctta ggggagttgc ctctaccgtg
     7141 tctgcctagt ataccagacg atgcttcgaa tggccacgat tctacgtccg cgttggggca
     7201 ggagcgcaat ctctttttct gcttttcgcc aatcagggtc ttttcgtgcc cctagaccgc
     7261 caactccgcg aattcctcga gcggaaacgc gtccacctgg atcgcgtccc gccgcgccac
     7321 gtccgcctcc cgccagatcc gccgctcgtc ctcgtcgatc acccccgcgg tcacggccgc
     7381 ggcaagctgt gcctccgcat cgagatgggc ggggatccgt cgagtccgga cggcgtcctt
     7441 gagcttccgg tcgatgggct gggccgccac caccttggcg taggccgcct ccagccggcc
     7501 gaggctcccc tccgatgcgg gggggatgta cacgtccgaa gtcatggcca gccggagggc
     7561 ccggtcgtcg agcagtccgc gggcgaccgc cgcgctcaac cggtccgcgg gcggcttggc
     7621 ccggaagccg agcgggaacg ccgcgagccg gaccagccag gccgtgggcc ggctcggcat
     7681 gttgtccagg acgccggaga tcgcggtctc gatctcgtgg aaggcgaaag cgcaggacca
     7741 gcgcacgagc ggcaggtcgc ggcggagctg tccctcgtcc acgaagcgct tgagcgccgc
     7801 ggaggaaaga tagaggtacg ccagcgcgtc cgccaggcgc ccgctcagcc gctcccgccg
     7861 cttgagcgtc ccgcccagcg tcgccatggc cgcttcggag gtgagcgcga acgccgcgct
     7921 caggtgggag agccttccga acagggggcg cgccggaccg ctgacgggga ctccctcgaa
     7981 ggccccaccg gtcagtccgt ccaggaacgc gcgacccacg gtggagacga cgtgtcccag
     8041 gtggccgaag aaggcgcgat cgaacgccgc caggtcgcgc cgggcgatgg cggccatctc
     8101 gcgctgcacg taggggtggc agcgaatcgc cccctgaccg tagatgatga gcgaccgggt
     8161 gaggatgttc gcgccttcca cggtgatccc gaccggcacc gcggcatagg cgggggccat
     8221 gacgttgcgc gggccgcgcg agatcgcggc acccgcctgg acgtccatcg cgtcgttcac
     8281 cacggcgcgc atcccctcgg tcaggtagcg cttcacgatg gccgagatga ccgcgggctt
     8341 ctgaccctcg tcgatcgcgc ccaaggagag ccggcgcgcc gcgtccatga ggtaggtgcg
     8401 cccgccgatc cgcgcgatcg gctcctcgat tccctcgaag cgtccgatcg gaaggccgaa
     8461 ctgctcccgc acgttcgcgt aggcggtgac cgttcgggtc gccagctggg cgcacccgac
     8521 cgcgagcgac ggaagcgaga tgccgcggcc ggacgccagc gtctccatca acatccgcca
     8581 tccgatgccc gcgttcgcgc tcccgccgat gatggcgtcg agcggggcga agacgtcgtg
     8641 cccctcggtc ggtccgttca ggaacggaac cccgagcgga tcgtggcgcg gtccgatcat
     8701 gatccctggg agattcgcgg gaaggagcgc gcaggtgatc ccgatctcct ccttgtcgcc
     8761 caggagccgg tcggggtcgc gcagcacgaa cgcgaggccg atcacggtcg cgacggggct
     8821 cagcgtgatg tagcgcttgg accagttgag acgcatgccg agcacttcgc gtccctggta
     8881 gatcccgcgc gtcacgatgc cgacgctctt ctgcgaggtc gcgtcgctcc ccgcctcggg
     8941 ctcggtcagc gcgaagcagg ggatgtcctc gccgcgcgcc agacgcggca ggtagtgccg
     9001 cttctgctct tcggtcccgt agtggaggag cagctcggcc gggcccagcg agttcggcac
     9061 catggcggtg accgacgcgg tgacgctccg gctcgagatc ttggtgatca cggccgagtg
     9121 cgcgttcgcc gagaagccga gtccgtcata ttccttggga acgatcatgc cgaagaagcg
     9181 gtgcttcttg aggaagctcc agacctccgg agggagatcg tgccccttca tgacctccca
     9241 ctcgttcagc atccggcaga gctcctcgac cgggcccgcc aggaaggcgc gctcctcctc
     9301 ggtgagcgcc ttggcgcgga acgcgaggag ccgcttccat tcgggacggc cggagaagag
     9361 ctcggcctcc caccagacgc tccccgcctc gagcgcgatg cgctcggtgt cgcccatgcg
     9421 ggggagcgcc ccggcgacca gcggcatcag gggccgcgtg atgaggttgc ggcggatcgg
     9481 gggcacgacg aggatcagca ccagcagggc gaggacgatc aggaacacga tcacgggtcg
     9541 gactcctgtc cttggggtac ggcgcgcgcg cgcccatcat aacccagcga atcccgtggc
     9601 ggggcgtcgt ttgaatccct attctcatcg gcctccatgg cgccgttccg aagcttttcc
     9661 atcgcggagg atatccgggt cgcatccacg cttcccgcgt gggtctactc cgaccccggg
     9721 gtcttcgcgg actgccgcga gcgggtcttc gtcccctcct ggcagctggc gggcgacacc
     9781 gaccgggtca agatccccgg gcaggtctgc cccgtgcccc tgctggaggg gatgatcgac
     9841 gagccggtcc tgctcacccg cgacgccgaa gaccgggtcc attgcctctc caacgtgtgc
     9901 acccatcggg gcaacctggt ggttgagagc gagggggtgg agcacgtcct gcgctgccgc
     9961 taccacggcc ggcgcttcgc cctggacggc cggttccact cgatgccgga gttcgaccgg
    10021 acggagggtt tcccctcggc ggcggacgat ctcccgcggg tccccttcgg aacgtggggg
    10081 aagttcctct tcgcctcgct gcgccccgcc gtgccgctcg agacgctcgt cgggccgatg
    10141 gacgcgcggt gcggctggct gccgatcgcc gaggcggccc tcgatccctc gcgcacgcgc
    10201 gagtacatcg tgcgcgccaa ctgggcgttg tactgcgaca actatctcga ggggttccac
    10261 atcccctacg tgcacgccgc gctctcggac gcgctcgact acggccagta cacgaccgag
    10321 acgcatgggc gctcggtgct gcaggtgggg gtggccaagg agggggagga cgccttcgac
    10381 ctgccgcgct cctcgcccga ccacggccgg aggatcgccg cctactactg gtggctcttc
    10441 cccaatctga tgttcaactt ctacccgtgg ggcatctccg tgaacgtggt ccgcccgctg
    10501 gacgtggacc gcacgaaggt gctcttcctc gcgtacgtgt gggatccctc gcgcctggac
    10561 cggggcgccg gcgcggccct ggatcgcgtg gagcgcgagg acgaagtcat cgtcgagagc
    10621 gtgcagcgcg gcgttcggtc ccgcctgtac gagcggggac gctactcgcc cacccgcgag
    10681 cagggggtgc accggttcca ccagctcctg gccgaggttc tgggctccga gcgctgaccg
    10741 gctccaacct aaccgctcca cggcctgtcg aaaccctacg caacgcacga ccggtcgaaa
    10801 cctcgagacg accgaagcgg tagtgagcgt ggggcccggc gacaggtccg agccttccgc
    10861 ctcgaactcc cccccgagtc cctggccatc gagccgcgta aggagatcgc acatgtctcg
    10921 cgcaggagcc cgaaccattc cagcctgcgt catgctggct ctctcgctcg tcctcgccct
    10981 cctggcgctc gcggcgccgc gggccgaggc actccccggc ttctcgccga ccgacgaggg
    11041 gccgtcgtac gccgactccg cggacggcag gaagtcggcg agcccgacga tccaggcgta
    11101 ccgtctcgac aatcaggaga tccgtctcga cggtcatctc gacgacgcgg cgtggcaggc
    11161 cgcgcccgcg gggcgcggat tcaccgtgtg ggagcccgac cgcggcgtga cgcccgcgga
    11221 gcagacggtc ttcaaggtcg cgtacgacga gggcgccgtc tacttcggga tcgcctgttt
    11281 gaagagcgat ccgaaggcga agatcgcggc cacgctgacg cgccgtgacc acatcacgaa
    11341 ctccgacatc gtggcgatct acctcgatcc ctaccacgac cacacgagcg gctacgcctt
    11401 caaggtgaac ccgctcgggg tgcagctcga ctcctacatc tataacaacg gcgacggcaa
    11461 cgacgacgac tgggacgcgg tgtgggaggc ggagacctcg cgcgacgacc aggggtggta
    11521 cgccgagatc cggatcccct tctcggcgat ccgctaccgc tcggccgagg acatgacctg
    11581 ggggctcaac gtctggcgct acatgaaggg gctgggtcag gacacggcct ggcacacctg
    11641 ggaccggaag caggccggct tcgtcagcca gttcggccag ctcacggggc tgcgcgggat
    11701 ccgcgcgccg cgccagatcg aggtgctccc gtacgtggtg cagcgcgaga ccgaccacgc
    11761 catgatctcc tccaagaccc ggcccgaccg cctggacggg ttccaggacg ccggagccga
    11821 cttcaagtac ggcgtcacgt cggacctgac gctgaacgcg acggtgcagc ccgatttcgg
    11881 ccaggtggag gccgatcccg ccgtgctcaa cctgtcgccg ttcgagacct cctaccagga
    11941 gaagcgcccc ttcttcgtcg aggggagccg cttcttccag catcccacgt tcaacctctt
    12001 ctactcgcgg cgcatcggca ccggcgacga gaacgcgcgg atccgctacg ccggcaagct
    12061 gaccggcaag accgtgggcg gaatctccgt cgccgcgctc gccgcctcca ccgacctcac
    12121 gcacgacggc caggcgcaca acatcttcaa gagcggcgac cggctctcgc gcttcttcgt
    12181 ggggcgcttc ggcaaggagt tcgcgcaggg gcgccaccag atcaacttca tgcagaccgg
    12241 cgttctcaac acggccgatc gctcgacctg gggcgaccgc gcctcgcgcg aggcctacac
    12301 cagcgggctc gatttcgccc tcacctcgct gcaccggtcg tggcgcgcca gcggctcctt
    12361 cgtgggctcg atcgtcaacc ccgagcccgc gcccggggtg agcgacgcgc cgcgctacgg
    12421 caccggtggc acgttccagg tcgaccgcat gggcgggaaa tggcgcggga acttcggcgg
    12481 ccggtgggaa tcgggcaagc tggacatcaa cgacatgggc tatctccagg ccccggacga
    12541 gatcgacagc tacgcgtggg cgcagcggcg gttcaatccc gaaggaaaga gccgcgtgta
    12601 caacagcgcc aatctcaact tcaacttcaa ccgctcctgg atgtacgcgg gacggaccgg
    12661 atacgacgtg ggcacggggc tgcccgcctg gcactatgaa ccggggcatc cgcagtacct
    12721 gacgtcgaac atcaacggct ggatgcagtt caagaatttc cgcgaggcct ggttcggcat
    12781 gctctacaac gactggggcc accatcgtta cgagacgcgc ggcggcccca tcatcagcga
    12841 gcccctcacg tacggcggct gggtgggcgc ctccaccgat cgccggaaga acctgaacgc
    12901 ctcgtccgag gtgagctgga acaaggacgt tgcgcagaac gtctcgctcg acctgaccac
    12961 cgacatcaac tggaaccagt cgagcgcggt caaccacacg ctcagcctgg gctatcacaa
    13021 cc
//
LOCUS       DASSLZ010000210         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1217865, whole genome shotgun sequence.
ACCESSION   DASSLZ010000210 DASSLZ010000000
VERSION     DASSLZ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217865"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFM00_08045"
     CDS             <1..306
                     /locus_tag="VFM00_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498238.1"
                     /translation="ALEATTAGMDDVPGGRVALPAGPGQAAAALTIIRIVIADPSTER
                     AFRAAAGSGSLTARPRRAPARAGSVETLVIELYGGRRDVEGVARRIPALALRRLLDR"
     gene            461..1213
                     /locus_tag="VFM00_08050"
     CDS             461..1213
                     /locus_tag="VFM00_08050"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HET7498239.1"
                     /translation="MRSRYRLVPHLAIGLLLLCGVALLGANASRTTPTSMNWAMNATI
                     IEACSCPMFCQCYFNTKPSEHMVMNGDAHSMEHFCKFNNAFRVNKGSYAGTKLDGAKF
                     WVAGDLGGEFSTGEMNWAVLTFDPSVTPEQREGIKAIMGHVYPVKWKAFTVAEDAPME
                     WNYTKDVATAKLDGGKAAEVSLKRYPGNSEQPITINNLKYWGTKRNNGFVLMPNEVEA
                     YRLGDKAFEYKGTNGFMITFDINNKDVVAAAN"
     gene            1287..1814
                     /locus_tag="VFM00_08055"
     CDS             1287..1814
                     /locus_tag="VFM00_08055"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7498240.1"
                     /translation="MNLAYTRTLWEEFRLVNGITLRVIDAIPADKIDAHPIPNMRTPK
                     ELVVHMAETMRGCSTGAIQGKITNFEEHEPVKVAAITTKDAMIAAMRSAWNEADAAVK
                     SMTESQATANVETPWGFNPWGWLCIQIIFDEHLHHRGQLYAYARALGVPEVPFMWDFA
                     HNAPEFRPNPNLQPA"
     gene            1848..>2767
                     /locus_tag="VFM00_08060"
     CDS             1848..>2767
                     /locus_tag="VFM00_08060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7498241.1"
                     /translation="MTSGRTRTGSSPFAFLWAAQFLSQLGDSIFQVAFIWLVLDITGS
                     KTLTGLAAAMAYLPSLLFGIAAGFLIDRWNRRRVLAGADLARAVLLVIGSVLLLQGRL
                     NAAGLAGVAFGMATASVLFNPARDSLLPELVPASRLPRANAWVQLSQQAAWLAGPLAA
                     GAVIAAAGVRSAFPFCAVLFGASFGFLALLGGAGRAHRGDTPLPGLARDFLDGMRWVL
                     ADRTLVVLLLLTALDNLFIMGPGLMTAVLVKDTLHLDAKAFAIAESAYGVGMILGSLL
                     VGRVLGPARQGWVLLVAIALDGFTYVPLYF"
BASE COUNT          434 a          975 c          905 g          453 t
ORIGIN      
        1 gccctcgaag ccacgacggc cgggatggac gacgtcccgg gtgggcgcgt ggcgcttcca
       61 gcgggccccg gtcaggccgc ggccgcgctc accatcatcc ggatcgtgat cgccgacccg
      121 tccacggagc gggcgttccg ggccgccgcc ggctcgggtt cccttacggc gcgtccccgg
      181 agggcgcctg cccgcgcggg ctcggtcgag acgcttgtga tcgagctgta cgggggccgg
      241 cgggatgtcg agggggtggc ccggcgcatc cccgcgctcg ccctccggcg cctcctggac
      301 cgctaggacc gcccccggga ggacggcgga agaaccgccc ccctcagtac ccccttccca
      361 aggggtatac caaagtagta ttaccttcct gggcaaacgg acggcacaac gccgctccac
      421 ccgcgttacc atccccactt catcgttcga aggaggtccc atgcgctccc ggtaccgtct
      481 tgtgccgcat ctcgccatcg gattgctgct tctctgcggc gtcgcccttc tcggcgccaa
      541 cgccagccgc accacgccga cgtccatgaa ctgggcgatg aacgccacca tcatcgaggc
      601 ttgctcctgc ccgatgttct gccagtgcta cttcaacacg aagccgtccg agcacatggt
      661 catgaacggc gacgcgcatt ccatggagca cttctgcaag ttcaacaacg ccttccgcgt
      721 gaacaagggc agctacgccg gcaccaagct cgacggcgcc aagttctggg tggccggcga
      781 cctgggcggc gagttcagca cgggcgagat gaactgggcc gtgctgacgt tcgacccgtc
      841 ggtgacgccg gagcagcgcg agggaatcaa ggcgatcatg ggccacgtct atcccgtgaa
      901 gtggaaggcg ttcacggtgg cggaagacgc tcccatggag tggaactata cgaaggacgt
      961 cgccaccgcc aagctggacg gcggcaaggc ggccgaggtc tcgctcaagc gctaccccgg
     1021 caacagcgag cagccgatca cgatcaacaa cctcaagtac tgggggacca agcggaacaa
     1081 cggcttcgtc ctcatgccga atgaagtgga ggcctaccgt ctcggagaca aggcgttcga
     1141 gtacaagggc acgaacggct tcatgatcac gttcgacatc aacaacaagg acgtggtcgc
     1201 cgcggcgaac tgacctcgcg ttgtgacggc gggcgccctc cgcgggcgcc cgccgctctc
     1261 tctcgccgga agaacaggag tccgcgatga acctcgctta tacccgcacc ctctgggaag
     1321 aattccgcct cgtgaacggc atcacgcttc gcgtcatcga cgcgatcccg gccgacaaga
     1381 tcgacgccca ccccatcccg aacatgcgca cgcccaagga gctggtcgtg cacatggccg
     1441 agaccatgcg cgggtgctcg acgggggcga tccaggggaa gatcacgaac ttcgaagagc
     1501 atgagccggt gaaggtcgcc gcgatcacga ccaaggacgc gatgatcgcc gcgatgcgct
     1561 cggcctggaa cgaagcggat gccgcggtca aatcgatgac cgagtcgcag gcgacggcga
     1621 acgtcgagac tccgtggggc ttcaatccgt ggggatggct ctgcatccag atcatcttcg
     1681 acgaacacct gcatcaccgc ggccagctct acgcgtacgc gcgcgcgctg ggcgtccccg
     1741 aggtcccctt catgtgggac ttcgcgcaca acgcccccga attccggccg aatccgaatc
     1801 tccagccggc ctaggcgcgc gcctcgcgcg aaggcgtgaa ggcgtccgtg acatcgggga
     1861 ggacccgcac cgggtcctcc ccgttcgcgt ttctctgggc cgcgcagttc ctctcccagc
     1921 tcggcgactc gatcttccag gtcgcgttca tctggctcgt cctcgacatc accggatcga
     1981 agacgctgac gggcctggcg gcggcgatgg cctatcttcc ctcgctgctc ttcggcatcg
     2041 cggccgggtt cctgatcgac cggtggaatc gccggcgggt gctcgccggc gccgatctcg
     2101 cccgcgcggt gctcctcgtg atcgggagcg tcctgctgct ccagggacgg ctgaacgcgg
     2161 cggggctcgc cggggtcgcc ttcggcatgg ccaccgcctc ggtgctcttc aacccggcgc
     2221 gcgattcgct gctgcccgag ctggtgcccg cgtcgcgcct tccgcgcgcc aacgcctggg
     2281 tgcaactctc gcagcaggcg gcatggctcg cgggaccgct cgccgcgggc gccgtgatcg
     2341 cggcggccgg cgtgcgctcg gcgtttccct tctgcgcggt tctcttcgga gcgtccttcg
     2401 gcttcctggc gctcctgggc ggcgcgggcc gcgcgcaccg gggggatacc cccctcccgg
     2461 gactcgcgcg cgacttcctg gacggaatgc gctgggttct cgccgaccgc accctggtcg
     2521 tgctcctgct gctcaccgcc ctcgacaacc tgttcatcat ggggcccggc ctgatgacgg
     2581 cggtgctcgt caaggacacg ctgcacctgg acgccaaggc gttcgccatc gcggaatcgg
     2641 cctacggcgt ggggatgatc ctcgggagtc tgctcgtggg gcgggtgctg ggtccggcgc
     2701 gccagggatg ggtgctcctc gtggcgatcg cgctcgacgg attcacctac gtgccgctct
     2761 acttctg
//
LOCUS       DASSLZ010000211         7991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1471481, whole genome shotgun sequence.
ACCESSION   DASSLZ010000211 DASSLZ010000000
VERSION     DASSLZ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471481"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="VFM00_08065"
     CDS             complement(<1..1517)
                     /locus_tag="VFM00_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET7498242.1"
                     /translation="MTPRTAVVLGAGPTGLSAALTLGDEAVLVEREPTVGGCCRSIVS
                     GGFTFDHAGHIMFSNDPYVHDLYRLLLGDNVHWQEREAWIYSKGVHTRYPFQGALYGL
                     PNQVIKECLIGAIEARFGPLKAPDPKTLKPMACAAVGGNGNGHAANGHGNGHANGHAN
                     GHANGHANGHSNGHANANGNGHLPVGDVKDCCADGILESEVPLAAPATLAEGDGAPWS
                     EPRNFEEFIHRVWGAGIAKHFAIPYNRKLWKVPLSEMETSWLGGRVPLPDLEEMIDGA
                     LSTVPKPMGPNARFGYPLRGGFQALMDGFLPRLRADLRLETEAVRLCPSKRMAWLSDG
                     STVEYETLISTIPLPRLVRLLGPEAPAAVRDAAAGLRHLSVRCVNLGVGRPDLTEKHW
                     IYYPEDTVFHRIFVQGNASPHCNPPGGFGLTCEITYSGDHPLPLDGDALTERCIADCR
                     RVGLVGDDDPIWTACQADLPYAYVIYDHERRERVEHIRAWLLERDIFLAGRFSEW"
     gene            complement(1514..2674)
                     /locus_tag="VFM00_08070"
     CDS             complement(1514..2674)
                     /locus_tag="VFM00_08070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7498243.1"
                     /translation="MEYTPVVVFSHLRWNSVFQRPHHIMTRLAERRDVLFVEEPVTSS
                     AVAGLEVMRVAPRVRVVQPPLPVEGPGFGPRQERILSAQLSRFLEREGYERFVAWLYT
                     PMAVSVAQGLNPAAIVYDCMDELSHFLHAPAGIQEREHELFECADAVFTGGPSLYRAK
                     QRLHPYVHCFPSSVDVKHFASAGSIPDAREQARLPHPRFGFFGVIDERMDLDILRHLA
                     AERPQWTFVMVGPVAKIERSALPWGANLRYMGQQPYHDLPGYLAGWDVCIMPFAKNDA
                     TRFISPTKVLEYMAADRPIVSTSIEDVAEPYRDIVFLGDGPEGFLQACERALSQTEAE
                     RASRRALAREVLARTSWDETVDRMDAILRRVAGDSAYREAPAYPAASVRSIA"
     gene            complement(2895..3641)
                     /locus_tag="VFM00_08075"
     CDS             complement(2895..3641)
                     /locus_tag="VFM00_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498244.1"
                     /translation="MKSIILTVSLLAAVVVIAAPALGKEPSPATPSPAPALFLSRVDQ
                     FMISTEEFSDWMAHHKTRLELRDANKAIEQMCDRLQDAERRMSTIASDPGIKPGDAQG
                     IAIQQFGDEIGTVARELTTLHASVRRIAEATPAGADSVVSTERQRLEGRQGELVAALD
                     LADKHAREAHAWMIEKPAARGLDEMSRDMDLSRDELRGIVTALGRLAADPVLSSDRVR
                     DLVQVQDCSRALLQALSEAQDRIEALARTP"
     gene            3961..5358
                     /locus_tag="VFM00_08080"
     CDS             3961..5358
                     /locus_tag="VFM00_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HET7498245.1"
                     /translation="MRFPAEPFRIKVVEPLRYVSREEREELLEKAGLNLFAVPADSIY
                     IDLLTDSGTSAMSDAQWAGLMVGDESYAGSKNFYRLERTVRELTGYKHVIPTHQGRMA
                     EHLLFSSVLKPGQIVPNNSHFDTTRANVEAQGAEALDLVIPEGQDPDADHPFKGNMDV
                     ARLERLLNEEGRAKVPLVMLTVTNNSGGGQPVSLANVRAVREVCDRHGVPLFLDACRF
                     AENAWFIQEREPGCAGKSVRQIAREMFDSAAGCTMSAKKDGLVNIGGFLAFQADALES
                     TVTNLLLRVEGFPTYGGLAGRDLEGMARGLVEVLEEDYLGFRIHQVRELGAMLMEGGV
                     PIVKPVGGHAVYIDARRFLPHVPQSRFPGQALVVALYRDYGIRGVEVGSLMFGHKDPT
                     TGEDVFPRLDLVRLAVPRRVYTYEQMRYVAQSVVEIYRKRETLHGLRLVYEAPVLRHF
                     TARLAPVQPEPAPTR"
     gene            5412..6566
                     /locus_tag="VFM00_08085"
     CDS             5412..6566
                     /locus_tag="VFM00_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HET7498246.1"
                     /translation="MRLALMLALAIPVLAPLSGVSCAAAAKTSKDPLASGALRATAVG
                     RYDFESADSRMDTPDPSADPTLTPEELSGLVRIEGDRYFAIGDAHATVHAMTITVDPR
                     TGAVRKASFDAPILLRDIPGSRLPEPAMAEDREGITYDPVTKEILIANEQTGADKRWP
                     SIERYGMDGKTVAVLRVDADPALAPFLHVRLNRGFESISRAEDGSYWTANEDALLVDG
                     PAASDSAGAEVRLLHLDAGLHPVAQFVYPLDPWSRKIRNPSIVSGKEISGLSELVALP
                     GGRLLALERAFGGDIGGYASLRSRLYVVDLAGATDVSTPEYRDGLAGKRYRPARKTLL
                     WEEGWGLTNSNFEGMALGPELADGSRLLLLIADNNGGSAQALFTLRLRKR"
     gene            complement(6603..7313)
                     /locus_tag="VFM00_08090"
     CDS             complement(6603..7313)
                     /locus_tag="VFM00_08090"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7498247.1"
                     /translation="MNDRNETDEARLEEIRQRAREMRVDEKIERSEPRSRRADRGPHV
                     SAWRSPAQGGHRDYGRYAYGDPGMVGYSGPVPVAYPMGGYPGYAGVPTPVGMPFEAGR
                     PGGSGFASDPARRWSSNERERSTRETWFPRARVFERDGALIVRLELPGLERKDVEVRV
                     EDQMLVVEGERPDDRPADGGVDPRSRWGYGPFRSEVALPHPVDADDVKARFRNGLLEV
                     AVPQPRREERRSRIKLET"
     gene            7531..>7991
                     /locus_tag="VFM00_08095"
     CDS             7531..>7991
                     /locus_tag="VFM00_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7498248.1"
                     /translation="MGAMTWRHMMQVREIMTREPACCSPDTKVQEVAKMMLEHDCGVI
                     PVVESSGSKRPLGVVTDRDIAARVVAEGRDPARTTARECMTSPVLTVTPDTEVARCCD
                     ILEQHQIRRVPVVDERGNVCGMIAQADIATFASERDTAEVVRSISQPSGSSP"
BASE COUNT         1206 a         2798 c         2757 g         1230 t
ORIGIN      
        1 tcccattcgc tgaagcgtcc ggcgaggaag atgtcgcgtt ccaggagcca ggcgcggatg
       61 tgctccaccc gctccctccg ctcgtggtcg tagatcacgt aggcgtacgg caggtcggcc
      121 tggcacgccg tccagatcgg atcgtcgtcg ccgaccagac ccacccggcg gcagtccgcg
      181 atgcagcgct cggtcagcgc gtcgccgtcc agcgggagcg gatgatcgcc cgaataggtg
      241 atctcgcagg tgaggccgaa gcctcccggc gggttgcagt ggggactggc gtttccctgc
      301 acgaagatcc ggtggaagac cgtgtcctcg ggatagtaga tccaatgctt ctcggtcagg
      361 tcgggccggc cgacgccgag attcacgcag cgcacggaga gatggcgaag gccggcggcc
      421 gcgtcgcgca ccgccgccgg cgcctcgggc ccgaggagcc gcaccaggcg cggcagtgga
      481 atcgtgctga tcagggtctc gtactccacc gtggaaccgt cgctgagcca ggccatgcgc
      541 ttcgaggggc agagacgcac ggcctcggtc tcgaggcgca ggtcggcgcg cagccgcggc
      601 aggaatccgt ccatcagggc ctggaacccg ccgcgcagcg gatagccgaa ccgcgcgttc
      661 ggacccatcg gcttgggcac cgtggagagc gccccgtcga tcatctcctc gaggtcgggg
      721 agcggcacgc ggccgcccag ccacgacgtc tccatctcac tcagcggcac cttccagagc
      781 ttccggttgt aggggatcgc gaagtgcttg gcgatgcccg cgccccacac gcgatggatg
      841 aactcctcga aatttcgcgg ctcgctccat ggcgcgccat cgccctcggc gagcgtcgcg
      901 ggcgcggcga gcggcacctc ggattccaga attccgtcgg cgcagcagtc cttcacgtcg
      961 ccgaccggca ggtgcccgtt gccgttcgcg ttggcgtgac cattggagtg gccgttcgcg
     1021 tggccgttcg catgaccgtt cgcgtggccg ttcgcatgac cgttcccgtg gccgttcgca
     1081 gcatggccgt ttccgttccc tccgaccgcc gcgcacgcca tcggcttcag cgtcttgggg
     1141 tcgggggcct tcagagggcc gaagcgcgcc tcgatcgcgc cgatcaggca ttccttgatg
     1201 acctggttcg gcaggccgta cagcgcgccc tggaacggat agcgcgtgtg gacgcccttg
     1261 ctgtagatcc acgcctcccg ctcctgccag tggacgttgt cccccaggag gaggcggtag
     1321 agatcgtgaa cgtagggatc gttggagaac atgatgtggc cggcgtgatc gaaggtgaag
     1381 ccgccggaga cgatcgagcg gcagcagccg cccaccgtcg gttcgcgctc caccaggacg
     1441 gcctcgtcgc caagcgtcag ggcggccgag agccccgtcg gtccggctcc caggacgacc
     1501 gcggtgcggg gcgtcacgcg atcgacctca ccgaggcggc cggataggcc ggcgcctcgc
     1561 ggtaggccga atcgccggcg acgcggcgca ggatggcgtc catccggtcc accgtctcgt
     1621 cccacgacgt ccgggcgagc acctcgcggg ccagcgcgcg ccgggacgcc cgctcggcct
     1681 cggtctgaga gagcgcgcgc tcgcacgcct ggagaaatcc ttccgggccg tcgcccagga
     1741 aaacgatgtc gcggtacggc tcggccacgt cctcgatcga ggtgctgacg atgggacggt
     1801 cggcggccat gtactcgagc accttcgtcg ggctgatgaa ccgcgtcgcg tcgttcttgg
     1861 cgaacggcat gatgcagacg tcccaccccg ccaggtagcc gggcagatcg tgatagggct
     1921 gctgtcccat gtagcggaga ttcgctcccc acgggagcgc ggagcgctcg atcttcgcca
     1981 ccgggcccac catgacgaac gtccactggg gccgctcggc cgcgaggtgg cgcaggatgt
     2041 cgagatccat gcgctcatcg atgaccccga agaagccgaa gcgcggatgc gggaggcgcg
     2101 cctgctcgcg cgcgtcggga atcgatccgg cgctcgcgaa gtgcttcacg tcgacgctgc
     2161 tcgggaagca atggacgtac gggtggagcc gctgcttggc gcggtagagg ctgggcccgc
     2221 ccgtgaacac ggcatcggcg cactcgaaca gctcgtgctc gcgctcctgg atcccggcgg
     2281 gggcatgcag gaagtgcgag agctcgtcca tgcagtcgta gacgatcgcc gcggggttga
     2341 gcccctgcgc caccgacacc gccatcgggg tgtagagcca ggccacgaag cgctcgtagc
     2401 cttcgcgctc gaggaaccgc gaaagctgcg cgctcaggat ccgctcctgg cgcgggccga
     2461 agcccgggcc ctcgaccgga agaggaggct ggacgacgcg gacgcgggga gcgacgcgca
     2521 tgacttcgag tcccgcgacg gcgctgctcg tcaccggctc ctccacgaag aggacgtccc
     2581 ggcgctcggc gaggcgcgtc atgatgtggt gtggccgctg gaacaccgag ttccagcgca
     2641 ggtgcgagaa gacgacgacg ggggtgtatt ccatgggagc gatctccggc gaaacgagtc
     2701 cgattgcggc gtggcccggt gcagatgcgg cgactgggca gcagcttcca tgccacgctg
     2761 aaaaccggag aatcgcgctg tcttcgtgag ttccgggagg gacgacgacg cccgaagcgc
     2821 gccggaccga agggcttaca aaaccgtggt cctctttaca cgccggcgcg cttcgcggta
     2881 cttcccttgc tgcgctacgg ggtcctggcg agcgcctcga tgcggtcctg cgcttcgctc
     2941 agcgcctgga gcagcgcgcg ggaacaatcc tggacctgga ccaagtcgcg gacgcggtcg
     3001 ctcgagagca cgggatcggc ggccagccgt cccagggccg tcacgatgcc gcgcagctcg
     3061 tcgcgcgaga gatccatgtc gcgggacatc tcgtccagcc cgcgcgccgc gggcttctcg
     3121 atcatccacg cgtgcgcctc acgggcgtgc ttgtccgcga gatcgagagc ggcgaccagc
     3181 tcgccctggc gcccctccag ccgctgccgc tccgtcgaca cgaccgagtc cgccccggcc
     3241 ggcgtcgcct ccgcgatccg ccgcaccgac gcgtgcagcg tcgtgagctc gcgcgcgacg
     3301 gtaccgatct cgtcgccgaa ctgctggatc gctatcccct gggcgtcgcc gggcttgatc
     3361 cccgggtcgc tcgcgatggt gctcatccgg cgctccgcgt cctgaagacg gtcgcacatc
     3421 tgctcgatgg ccttgttcgc gtcgcgcagc tcgaggcgcg tcttgtggtg cgccatccaa
     3481 tccgagaact cctcggtcga gatcatgaac tggtcgacgc ggctcaggaa cagagccggc
     3541 gcgggcgacg gcgtcgcggg cgacggttcc ttccccagcg cgggcgccgc gatcacgaca
     3601 acggccgcga ggagcgatac cgtgaggatg atcgatttca tggctccccc tttcgggccg
     3661 cgtgcgcggc cccttgcggc aggatggaac tgcaaccgcc gtgccgcctc ccgactggcg
     3721 ggacggggtc tccgtggttc cggcaacgcg cgtcccgcga agcgcttggg gaaggcgccg
     3781 ctccgtgagg tggcgccggt gccgctgccg cgggtgcctc ggtcctgaca cgctggcagg
     3841 ccggccacga gcgacctgcc accaccttcc gcgcgtgcag gtcagcggac cgaggccgga
     3901 gccgcgccgc taccaaggga ccatgtcgca cgggcgtccg gctccgttat ggtggagggg
     3961 atgcgattcc cagccgagcc gttccggatc aaggtggtcg agccgctccg ctacgtatcg
     4021 cgcgaggagc gcgaggagct cctcgagaag gcgggcctca acctgttcgc ggtcccggcc
     4081 gactcgatct acatcgacct gctcaccgat tcgggcacgt ccgccatgag cgacgcccag
     4141 tgggcggggc tcatggtggg cgacgagtcg tatgccggga gcaagaactt ctaccgcctc
     4201 gagcgcaccg tgcgcgagct caccggctac aagcacgtga ttccgacgca ccagggccgt
     4261 atggccgaac acctcctctt ctcatcggtg ctgaaacccg gccagatcgt tcccaacaac
     4321 agccatttcg acacgacccg cgcgaacgtc gaggcgcagg gcgccgaggc gctcgacctg
     4381 gtcatcccgg aaggacagga ccccgacgcc gaccacccct tcaaggggaa catggacgtc
     4441 gcccggctcg agcgcctgct gaacgaggaa ggccgcgcga aggtccccct ggtgatgctc
     4501 accgtcacga acaactcggg gggcggacag ccggtctcgc tcgcgaacgt gcgcgcggtg
     4561 cgcgaggtct gcgaccgcca cggcgtgccg ctcttcctgg acgcctgccg cttcgccgag
     4621 aacgcgtggt tcatccagga gcgcgagccg gggtgcgccg gcaagtcggt ccgccagatc
     4681 gcgcgggaga tgttcgacag cgccgcgggc tgcacgatga gcgcgaagaa ggacgggctc
     4741 gtgaacatcg gcggcttcct ggcgttccag gcggacgcgc tcgaaagcac cgtcacgaac
     4801 ctgctcctgc gcgtcgaggg gttccccacg tacggggggc tggccgggcg cgacctggag
     4861 gggatggcac gagggctggt cgaagtgctc gaggaggact acctgggctt tcgcatccac
     4921 caggtgcgcg agctgggagc gatgctgatg gaaggcggcg tgccgatcgt caagccggtc
     4981 ggcggccacg cggtctacat cgacgcgcgg cgcttcctcc cccacgtgcc gcaatcgcgc
     5041 tttccggggc aggccctggt cgtcgccctc tatcgcgact acgggatccg cggcgtggag
     5101 gtgggaagcc tcatgttcgg ccacaaggat cccacgacgg gcgaagacgt cttccccagg
     5161 ctggacctgg tccggctggc cgtgccgcgg cgggtctaca cctacgagca gatgcgctac
     5221 gtcgcccagt cggtggtgga gatctaccgg aagcgggaga cgctgcacgg cctgcgcctc
     5281 gtctacgagg ccccggtgct ccggcacttc accgcgcgcc tcgcccccgt ccagccggag
     5341 cccgccccta cccgatagga cgtcgcttct cggctggatt ccggggcgcc gcggccccat
     5401 gctcatcctc catgcgtctc gctctcatgc tcgccctcgc gatccccgtt ctcgccccgc
     5461 tctccggcgt ctcctgcgcc gccgccgcca agacgtcgaa ggatccgctg gcctcgggcg
     5521 ccctccgcgc caccgccgtc ggccggtacg acttcgagtc cgccgactcc cggatggaca
     5581 cgcccgatcc ctcggccgat ccgaccctaa ctcccgagga gctgagcggc ctggtgcgga
     5641 tcgaggggga ccgctacttc gcgatcggcg acgcgcacgc caccgtgcac gcgatgacga
     5701 tcacggtgga cccgcgcacc ggagcggtgc ggaaggcttc cttcgacgcg ccgatcctcc
     5761 tgcgggacat ccccggctcg cgcctgcccg agcccgcgat ggccgaggat cgcgagggaa
     5821 tcacgtacga ccccgtcacg aaagagatcc tgatcgcaaa cgagcagacg ggagcggaca
     5881 agcggtggcc gtcgatcgag cggtacggga tggacggaaa gaccgtggcg gtgctgcgcg
     5941 tggatgcgga ccccgcgctc gcccccttcc tgcacgtgcg gctcaaccgg ggattcgagt
     6001 cgatctcgcg cgccgaggac gggagctact ggacggcgaa cgaggatgcc ctcctcgtgg
     6061 acggtccggc cgcgtccgat tccgccggcg ccgaggtccg gctgctgcac ctggacgcgg
     6121 gcctccatcc ggtggcgcag ttcgtctatc ccctcgatcc ctggtcccgg aagatccgga
     6181 atccctcgat cgtctcgggc aaggagatca gcggcttgag cgagctggtg gcgctgcccg
     6241 gcgggcggct gctcgccctc gagcgggcgt tcggcggcga catcggcggc tatgcgagct
     6301 tgcgaagccg gctctatgtc gtcgacctcg ccggcgcgac cgatgtgtcg acgcccgagt
     6361 accgggacgg gctcgccgga aaacgctacc gcccggcgcg gaagacgctc ctctgggagg
     6421 agggatgggg tctcaccaat tccaatttcg aggggatggc gctgggcccg gagctcgcgg
     6481 acggaagccg cctgctcctc ctgatcgcgg acaacaacgg cggctccgcc caggcgctct
     6541 tcacccttcg gcttcgaaag cgctgatgcg cccgcggcgc acgcgccgcg agcgctaccg
     6601 gttcacgtct ccagcttgat gcggctccgc cgctcctcgc gccggggctg cggcaccgcc
     6661 acctcgagca ggccgttccg gaaccgcgcc ttcacgtcgt cggcgtccac cggatgcggc
     6721 agggcgacct cgctgcggaa cgggccgtaa ccccagcggc tccgcggatc gacgccgccg
     6781 tccgcgggcc ggtcgtcggg ccgctcgcct tccacgacca gcatctgatc ctcgacccgg
     6841 acctccacgt ccttccgctc gagccccgga agctcgaggc ggacgatgag cgcgccgtcc
     6901 ctctcgaaga cccgcgcgcg cgggaaccac gtctcgcgcg tcgagcgttc gcgctcgttc
     6961 gagctccacc ggcgcgccgg atccgaagcg aagcccgagc caccgggacg ccccgcctcg
     7021 aacggcattc ccacaggggt cggcacaccc gcgtatcccg ggtagccgcc catcgggtag
     7081 gccaccggca ccgggccgga gtagccgacc atgcccggat cgccgtacgc gtagcggccg
     7141 tagtcccgat gtcctccctg agccggcgac cgccacgccg aaacatgcgg gcccctgtcc
     7201 gcacgccgcg atctcggctc gcttcgctcg atcttctcgt ccacccgcat ctcgcgggcg
     7261 cgttgccgga tctcttccag cctggcttcg tcggtctcgt tgcgatcgtt catggtgtcc
     7321 cttctttctt atcctctttc ctatgaaccg cgcgtcatcc tatgaactgc gcagcttcga
     7381 tctttgcaag aggcgcgccg aattgcgcga tgtcgagtgg ggcgtgcggc gtggtccttg
     7441 cgagggggcg gtgacggcat acgacggcgc taagaaacgc gctgttgcgg atacgaccta
     7501 gggttatggc gccctgctgg gcgccatgac atgggcgcca tgacatggag gcacatgatg
     7561 caggtccgtg aaatcatgac ccgggagccg gcatgctgct cccccgatac caaggttcag
     7621 gaagttgcca agatgatgtt ggagcacgac tgcggcgtga tcccggtcgt cgagtcgtca
     7681 ggctccaagc gtcccctcgg cgtcgtgacc gaccgcgaca tcgccgcccg cgtggtcgcc
     7741 gaaggacgcg atcccgcgcg gacgacggcg cgggagtgca tgacctcgcc cgtgctcacc
     7801 gtgacgcccg ataccgaggt cgcccggtgc tgcgacattc tggagcagca tcagatccgc
     7861 cgcgtgccgg tggtggacga gcgtggaaat gtgtgcggga tgatcgcgca ggcggacatc
     7921 gccaccttcg cgtccgagag ggacacggcc gaggtggtgc ggagcatttc gcagccctcg
     7981 gggagctcgc c
//
LOCUS       DASSLZ010000212        17773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1077159, whole genome shotgun sequence.
ACCESSION   DASSLZ010000212 DASSLZ010000000
VERSION     DASSLZ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 17773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17773
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077159"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFM00_08100"
     CDS             complement(<1..371)
                     /locus_tag="VFM00_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498249.1"
                     /translation="MKPTRLSGRSLALVFVLALAIPAPPASAQYMYMDSNGDGIHSDA
                     DEIHPTGTTTIDFYVRTDQNRDGSPAACPVAGASFTINSYVVNLEATNGTVAWGAFVN
                     HQPTMVVYVTGGTSATQFSQT"
     gene            634..708
                     /locus_tag="VFM00_08105"
     tRNA            634..708
                     /locus_tag="VFM00_08105"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:666..668,aa:His,seq:gtg)
     gene            875..2758
                     /locus_tag="VFM00_08110"
     CDS             875..2758
                     /locus_tag="VFM00_08110"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7498250.1"
                     /translation="MKRLPGLVAMVVLVTSMMPGIGYAGFRGAFDPIGRADIAAADGL
                     RFGLGLANGEQTPTARPMPVGEGNGIPLLERWITFTTRDQVVGFEGSREIWQMLNRTQ
                     PGTLVATIAEARSKVLGDPTGVVSYVDPKWSPNGKYLAYVLTNTRVTQSQILVQEFTV
                     STDMFTSITPVGAPITVTPMVAGVRDRHPDWSPDGDSLVFDSNRSGFSADVYTVSVFP
                     TVGIPVRHTFLNERAEQNPVWAPDGSNRVAFDTNAFGPNVIEIVDLNTDAVSLAETNL
                     VDVSHSHPGWSSDGASIYYDAPEEEDPQRNPDIWKLDLASQAKCPIHMDGAGDVNVSV
                     SRYQNVTPDGKKYNNIYFESQAFAGSLGTPNLIVWRANPIQSCEAPLPIAIEFSPSTF
                     NFDAGNANHGQTEKITATLSFPPETQAAGYQALSFNGPREGLRLRVSGLFSSPLLFGS
                     LRAMDEDPADVFPDGNGPGGNFKDSPSHGKVSMYFSARDIQSRLVSLGLVNQDIPVQV
                     NAYSNIVGRTFQGFGIFHVSSSSLAGSAVKLEQNAPNPFNPVTTIRFAVSRDSRVALR
                     VYNVRGQLVKTLADERMAQGMHEVNWDGRDTSGSHVASGVYYARVASEGGATDVIKMV
                     MAK"
     gene            2769..3851
                     /locus_tag="VFM00_08115"
     CDS             2769..3851
                     /locus_tag="VFM00_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7498251.1"
                     /translation="MTEDLGSDPRRDLYFIVRAIAFLGLILFLLWLAGDVLLLLFAGV
                     LVAIFIDGIANWIRIHTRVGRGASIAIALLGLVLVFSVVGIYSAPDIADQVGQLGQEL
                     PRAVDAVRQQLDKHAWTKLIIDQLPSAKELRSPGTGLLRRATGALSSTAIALARMVLI
                     AAIGVFLTLQPGLYRRGFLRLIPIRKRGRAADVLDAVTNNLRGWLVGKVLSAIAIGVA
                     TWTGLQLLGIPLALILSVLAGVFSFIPNVGPVLAVVPAMLLGLLQSPSLALYVFLLYL
                     GIQIVETYLLTPWLAHRTADLPPALTLVTQILLGVLFGGMGVIMAAPVTAAGMVLMRM
                     VYVEDVLGDKGAQENLPPRDRVGSSG"
     gene            complement(3848..5065)
                     /locus_tag="VFM00_08120"
     CDS             complement(3848..5065)
                     /locus_tag="VFM00_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7498252.1"
                     /translation="MSAKPTVAGAAAARAPAIPSCPVPDPLAPGAVVALLADLVRIPS
                     TNPAIAPDEAHGEGAIAEFACGWLLANGIRAWKEDAAPGRPNVAAEIGEGGPALILYG
                     HLDTVGTAGMTIPPFEPRVEAGRLYGRGATDMKGGVAACMAAAAALARERLPGRVLLA
                     LVADEEYESVGAADFVRRHRADGCIVSESSEGDLILAHKGFVWADITTRGRAAHGSRF
                     DLGVSAIARMGRIIAALDRFDADTLRTRTAPLVGPASMHCALVRGGVGLSTYAPDCSL
                     SIERRTLPGETGDQVEEELKRVIQEAGEEAEVSIRTVRRASHTAADSPLAVSVRDAVR
                     AVTGKVPRDKGAPYWMDSALFAEAGIPTVNYGPTGASPHEAVEWVDVASVVGCAAIYV
                     EAAHRFWGARQED"
     gene            complement(5062..5739)
                     /locus_tag="VFM00_08125"
     CDS             complement(5062..5739)
                     /locus_tag="VFM00_08125"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7498253.1"
                     /translation="MSRAAYQVRPPITRTPLRSEVRRLLLGEIVRGDLAPSEGINEAE
                     LSTRLGVSRTPLREAMLGLTQEGFLTATPGRGFSVRALRRSDAEEIYPILWTLEGAAL
                     RASWPLPPSRVRQLDQANRRLEEALAEASVALARDREWHALLLAGCPNRMLLSMIENL
                     KDHAARYEEAFMRNSGQVITSVTQHREIQTAIRRGDLERALALLEKNWRVSLDFLGPW
                     LEATASS"
     gene            complement(5736..6197)
                     /locus_tag="VFM00_08130"
     CDS             complement(5736..6197)
                     /locus_tag="VFM00_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HET7498254.1"
                     /translation="MKWITRENAKVDRIACPWLIRRFVDPEAVFLFVPADQVTAVAAA
                     EAAIPYDVPGAELGHVEGRCSFESILLKYGLTGDPGLEGVAAIVHAADVADDIDRAPE
                     ARGLRAIAEGFALVHGRDDRTKLEREAPLYDALYAWCRQRAAAGSPGASVS"
     gene            complement(6301..7068)
                     /locus_tag="VFM00_08135"
     CDS             complement(6301..7068)
                     /locus_tag="VFM00_08135"
                     /inference="COORDINATES: protein motif:HMM:NF004836.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET7498255.1"
                     /translation="MAAPIRIAIVGDYNPDFVSHQTTGAAIEHAAAKLGVPVEVEWIG
                     TASIAPDNPESVLADWDGLWINAGSPYASRPGALAAIRYGREGKRPMLATCAGFQYGL
                     LEFAHNVLGRTDLEHEEDAPEAENLLIHQVSCPVPGREDDAPKLSGPAQRVHIREDSR
                     AREILGTDRIEEEYFCNFELNPDYDELFEEHGLEFTGFGDRGDSRIYEVPEHPFYIAT
                     LFQPQRKSRPNAPHPLVVAFVRASARELAGSGEPAVR"
     gene            complement(7255..7857)
                     /gene="rdgB"
                     /locus_tag="VFM00_08140"
     CDS             complement(7255..7857)
                     /gene="rdgB"
                     /locus_tag="VFM00_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET7498256.1"
                     /translation="MIERVVLATRNAGKARELQTLLQGIAARVESLDAYPGVTLPPEG
                     HESFAENARAKARAVSEALAVAAIGDDSGLEVDALGGEPGIRSARYAGEAAGDAANNA
                     LLLERLVGVPPERRTARFRCVLALEAPGAPGALVEGVCEGLIAEAPRGTGGFGYDPLF
                     VPNGETRTFAELPGGTKDAISHRARAAAALREALGAGKGF"
     gene            complement(7854..8681)
                     /gene="rph"
                     /locus_tag="VFM00_08145"
     CDS             complement(7854..8681)
                     /gene="rph"
                     /locus_tag="VFM00_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET7498257.1"
                     /translation="MVRTPGELGAMSPARQESRQATRQAPRPGRRVDGRRAAELRPVS
                     IRRGYLKNAEGSALIEMGGTRVLCAATVEERVPPWLRGGGRGWITAEYSMLPRSSHDR
                     IQREVNRGHVGGRTQEIQRLIGRSLRAVANLEALGERQILIDCDVIEADGGTRTASIT
                     GAFIALADAIRWLSARVKLPGPPLRDFVAAVSVGLVNGTPSLDLCYAEDSKAQVDMNL
                     VMTGGGRLVEIQGTAEGEAFSERELAQLIGLGKAGIRRLIAEQKKVLGIRTLPKTWA"
     gene            complement(8648..9544)
                     /gene="murI"
                     /locus_tag="VFM00_08150"
     CDS             complement(8648..9544)
                     /gene="murI"
                     /locus_tag="VFM00_08150"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET7498258.1"
                     /translation="MRLGVFDSGIGGLTVVRHLRRLLPSWEIVYFGDTARVPYGAKSD
                     ATVRRFASEAVRFLSQFHVSRIVVACNTVSAVALRHLEREFQGLPVTGVIVPGADAAV
                     RATKNGRIGVIGTRATVASAAYQHAIAAASRRAGVRTRVWAEPCPLLVPFAEEGLQNS
                     KGAREVLREYLAPLARRRIDTLILGCTHYPVFKRAIRAVLGGRVRLIDSGEATARRLA
                     AAVRAEARAAGRSARGGKAARALARVARADGRGSLECFVSDIPQQFELVGRRFLGKAM
                     GRVRNVPQDDLPWFERPASWAR"
     gene            complement(9546..10136)
                     /locus_tag="VFM00_08155"
     CDS             complement(9546..10136)
                     /locus_tag="VFM00_08155"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HET7498259.1"
                     /translation="MSETFGSPGPGAVARRRWIVGGIAALVGIAIALLIWKAPWRRNE
                     LGLSRYSPVAAELEGVRGVYLYYALPNSDSLVAEYRDVVVKDHPVDQVRAIFRELIAG
                     PSGNRVSPFPDGTELLNTYWTQRGTLYLDWNRALVAGFRGGTGRERQLLGSIVKSAVE
                     NLPEVQRVTLLVEGNPIETIGGHFDTLQPLEAEDWR"
     gene            complement(10133..11602)
                     /locus_tag="VFM00_08160"
     CDS             complement(10133..11602)
                     /locus_tag="VFM00_08160"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7498260.1"
                     /translation="MRDVPTRVSLLLLLLLLLAALVPALTRAECSAAPITVIYPEPRN
                     PERFVPIVLDGVRYVSTNDLARVFGATKYWRPEIQKLSLRIGEHTLRFSIGAPVVLVD
                     EAAKNLIRPATLVRGVVYAPEDIVERIFDWGLASDAAWDETGRTIRFRGVVHSVRQAQ
                     LFVRGRVTEVSATLGRSLPARLLYAMPGEVRVLFEGGTLDTAHVFTGGAVARGEIREI
                     PGGVDLRLVLGDSAVGYQVSSAPNRMKVAVTDDPGLVEAGLFTPLEPLRVAGKANAVR
                     TIVIDPGHGGTDLGAQLPGGMAEKDATLEIARSLRTALASRLNAKIVLTRESDVLLSP
                     ARRAELANEAGADLFISIHLEGDGTIKGGGFRLLAESPVASPNEGGGVPVVLPGDGDG
                     IELKPWRSAQSAVVGASIAFAQTVADALARSFPQAPVLVRTGGMSVLEPVLCPAIVVE
                     SAPAARSGPESMSMRGYTTYDYTQTVARAIEDFVRKSRA"
     gene            complement(11602..12111)
                     /gene="smpB"
                     /locus_tag="VFM00_08165"
     CDS             complement(11602..12111)
                     /gene="smpB"
                     /locus_tag="VFM00_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET7498261.1"
                     /translation="MARDHDSKPKQDKPKREDERRVIATNRKARHDYWVIETYETGIE
                     LRGSEVKSLRDAKAQLQDAYATIEGGQLFLRGAHINPYDPAAHENHDPTRARRLLMHR
                     AEIRRLTTKLEESGLTLVPLSLYFKNGKVKVELALVRGRREFDKRAKLDERRSRRELR
                     GVMRGERPD"
     gene            12249..12335
                     /locus_tag="VFM00_08170"
     tRNA            12249..12335
                     /locus_tag="VFM00_08170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12283..12285,aa:Leu,seq:cag)
     gene            12798..14582
                     /locus_tag="VFM00_08175"
     CDS             12798..14582
                     /locus_tag="VFM00_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498262.1"
                     /translation="MTISRDGAFLGCHFSDEYLAQVQSWIRDKIGPVPDTTLLKQVLE
                     LQRSPEMRAKLEAKSGEVWNAWVGHWIESTLDPGMQVDARMPLPWISGTIESDVQFQN
                     HGPTRTGGRLDRLVWVTRTTGEEARLAYERAVRETLSKMPDLGTPPRTPLLGVIRTTS
                     LEIVLDPVSMQPNYVHEETKLDLQDSVAVHSSQEAKSYFFEWDPPLAAALDPVPPEGI
                     SAFLENRLPDAYFGLRTDVENGTRNPDTYAYLAETCRRLGQFGEGVLFARRALALNSR
                     HAFAHEVLGNCYNGQYGPWPLASRDSTWTHLLLAVESDSTDGNAWLGVFLESSRRGRN
                     DLEGRSLSKLLETGFLTRPILARARWMLQDLPDSAVLVTNGDMDSYPLWALQMRDRLR
                     PDVAVVNRSLLEAPWYARLVRDRLRVPLPFGDSVLDSLTPEGADSNVVYVSDRIIRGW
                     LDQQASGIFQRPLAFAFTIPEGWFSPSCAARSALSGPFYLCRATPAPSPLDTTAIRIS
                     LGHARASDFAGPYFSERDRSPVRRAAVSPAEDVVEMWFRYADALGRAGRRRDCLRALE
                     KTSAFAREAGTSPAWRAMMEKMRDRYSH"
     gene            14611..15174
                     /locus_tag="VFM00_08180"
     CDS             14611..15174
                     /locus_tag="VFM00_08180"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7498263.1"
                     /translation="MDNTTLTLDQLRDAGRNVSAADHPGTIRAYYPFWDTQYRPFLLA
                     AVAALPREKFDFKPRPEMFTAHEMIVHIAECERHWIHVVVEGGAYEEWVAPAEDPKQG
                     WMNVLDLPDHASLFAALEKWHRNTQQQLERPASDLSRRITWRAPSGREHTYTLHWILD
                     HLQEHEIHHRGQLNMYLRMMGIEPPSI"
     gene            complement(15198..15377)
                     /locus_tag="VFM00_08185"
     CDS             complement(15198..15377)
                     /locus_tag="VFM00_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498264.1"
                     /translation="MEGKDAIWLLVMICMLLAVMTLAGKIEPKIAGGAFAAALAILGV
                     LSRGFRKRSHKPLHS"
     gene            complement(15515..>17773)
                     /locus_tag="VFM00_08190"
     CDS             complement(15515..>17773)
                     /locus_tag="VFM00_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7498265.1"
                     /translation="RIWNAARTQSDIQGTINAQISTPAAGLVARWALDEGSGTAVGGS
                     AGTSVNGTITGTAYAWVSGAPFNLAFNLPPSAPALVGPADQATDVPLSPTLQVSAADP
                     DGQSLTVQFYGRSAVSSPGTDFTIIGMPDTQYYTGELNGGTNAILLSQTNWITANRAS
                     RNIGYVATLGDCVEHGDNGGNDIEWQHADAGYSLIENPLTTGLPEGLPYGITVGNHDQ
                     SPNGNPDGATTVFYNQYFGANRFTGRTYYGGHYGSNNDNWFNLFRAGGMDFVVISLEY
                     DTTPDAAILDWADGLLTTYANRRAIVLSHNFIGTGNPGAWSAQGQATYDRLKTHSNLF
                     LMLCGHVPGEGRRSDTFNGNTVYTLMSDYQGRTNGGNGWLRIMQFSPANNVIHVSTYS
                     PWLNQTENDADSQFDVPYSMSAAEPYALLGTSAGVASGGTASLVWSGLAGGMPYQWYT
                     TVSDGVATVTGPTWGFTTQVVPTHTLAVVSAPAAGGAVTPNPNRAAYDEGSVVQLTAS
                     PASGWEFRSWCGDAAGSANPISLTMSRDKRVTAIFNEIGAPLVAVSAPNGGEALAIGS
                     LYEIRWSAEDAEGVELVDVLLSRNGPSGPFETLGEGIENTGSFLWNVSYPGTANGYVK
                     VVAHNPAVLSPSLSGFDLSDAAFTIPDLVTGVDDQPVTAFSMRMTSSNPTSNGASLAF
                     ELPRTERVRVELFDAAGRLVETLADAAYPMGRHSVRWGGRTPHGQAPSGVYFVRFRAP
                     GHTITRRFVLVR"
BASE COUNT         2744 a         6134 c         5983 g         2912 t
ORIGIN      
        1 tgcgtctggc tgaactgggt ggcgctggtt ccgccggtga cgtatacaac catcgtgggt
       61 tgatggttca cgaaggctcc ccaggccacg gtgccattcg tggcctcgag gttcacgacg
      121 tacgagttga tggtgaacga cgccccggcg accggacacg cggccgggct gccgtcacgg
      181 ttctggtcgg tccggacgta gaaatcgatc gtcgtggtgc cggtcggatg gatctcgtcg
      241 gcgtcgctgt gaatgccgtc gccgttggag tccatataca tgtactgcgc ggatgctggg
      301 ggtgcgggga ttgcgagagc gaggacgaag acgagagcca gggatcgccc cgaaaggcgt
      361 gtgggtttca cggttgtctc ctgtcgcgcg cgccggacta cgaggggggt aggaggggcg
      421 gaaaccatcc taggcctcct cgaaccgcaa agcgaccatg aatcctcagg gcctggaacg
      481 ccgtcctgaa gtgcttggag gtgatatgct tacaggtgga ttcgaccccc tcctcgcgcg
      541 gggggcggtg tggtaccatc gcgtgctcgt ctcacccaga tgttccgcgg tgagcgttcg
      601 cagacgtatc gactccgcga acattcgacg gtggtggatg tagctcaatg gtagagcact
      661 ggactgtggc tccagtggtt gcgggttcga tccccgtcat ccaccccatt tcccccatcg
      721 catcaacggg aggccgcggg agcgcaccgc ggccccttcg tttgtagggt ggcttggacg
      781 ggcgtagtat cccatctcgt cggaccggat cccggtgcga ccttatcctc tcggggcgtc
      841 ttctcgctta cccgcattgg aagaggagat cgagatgaag cgattgccag gtctcgtcgc
      901 catggtggtc ctcgtcacct ccatgatgcc cggcatcggt tacgcggggt tccgcggcgc
      961 gttcgacccg atcggtcgag ccgacatcgc cgccgcggat gggctccgat ttgggctcgg
     1021 tctcgccaat ggcgagcaga cgccgaccgc gcggccgatg ccggtcggcg agggtaacgg
     1081 cattccgctg ctcgagcggt ggatcacatt caccacgcgt gaccaggtgg tgggtttcga
     1141 gggttcccgt gagatctggc aaatgctcaa ccgcacccag cccggaacgc tggtggccac
     1201 gatcgccgag gcccgctcga aggtgctggg cgacccgacg ggcgtcgtct cctacgtcga
     1261 tcccaagtgg agcccgaacg ggaaatacct cgcgtacgtg ctcacgaaca cccgcgtcac
     1321 gcagagccag atcctcgtcc aggagttcac ggtcagtacc gatatgttca cttcgatcac
     1381 gccggtgggc gctccgatca cggtcacgcc gatggtggcg ggcgttcggg atcgtcaccc
     1441 cgactggagc ccggacggcg actcgctcgt gttcgattcc aaccgctcgg gcttctcggc
     1501 ggacgtctac accgtttcgg tgtttccgac ggtgggaatc ccggtccgcc acacgttcct
     1561 gaacgaacgc gcggagcaga acccggtgtg ggctccggac ggcagcaatc gcgtcgcctt
     1621 cgacacgaac gcgttcggcc cgaacgtcat cgagatcgtc gatctgaaca cggacgcggt
     1681 cagcctggcc gagacgaacc tcgtcgacgt ctcccactcg catccgggtt ggagcagtga
     1741 tggcgcctcg atctactacg acgcgccgga ggaggaggat ccccagcgga acccggacat
     1801 ctggaagctg gacctggcct cgcaggccaa atgcccgatc cacatggacg gcgccggcga
     1861 cgtgaacgtc agcgtttcgc gctaccagaa cgtcacgccg gacgggaaga agtacaacaa
     1921 catctacttc gaatcccaag ccttcgcggg ctccctgggc accccgaacc tcatcgtgtg
     1981 gcgcgccaat ccgatccaga gctgcgaggc gccgctcccg atcgcgatcg agttcagccc
     2041 gtcgaccttt aacttcgacg ccggcaatgc gaaccatggg cagaccgaga agatcacggc
     2101 gaccctgtcc ttcccgcccg agacgcaggc tgccggctac caggctcttt cgttcaacgg
     2161 tccccgtgag ggcctccggc tccgtgtgag cggactcttc tccagtccac tcctgtttgg
     2221 ctccttgcgc gccatggacg aagatcccgc cgacgtgttt ccggacggca acggcccggg
     2281 cggcaatttc aaggacagcc cttcgcacgg caaggtctcc atgtacttca gcgcgcggga
     2341 cattcaatcg cgcctggtgt cgctcggtct cgtgaaccag gacataccgg tgcaggtgaa
     2401 cgcctactcg aacatcgtcg gccgcacgtt ccagggcttc ggcattttcc acgtttcgtc
     2461 gagcagcctg gccggctcgg ccgtgaagct ggagcagaac gcgccgaacc cgttcaaccc
     2521 ggtgaccacg atccgcttcg cggtgagcag ggattccagg gtggcgctgc gcgtgtacaa
     2581 cgtgcgcggc cagctggtga agacgctcgc cgacgagcgg atggcgcagg gcatgcacga
     2641 agtgaactgg gacggccgtg ataccagcgg cagccacgtc gcttcgggcg tctattacgc
     2701 ccgcgtcgcg tccgagggtg gagcgaccga cgtgatcaag atggtgatgg cgaagtaatc
     2761 cgcctcacat gaccgaagac ctcggctccg atccacggcg cgatctctac ttcattgtcc
     2821 gagcgatcgc gtttctcgga ctcatcctgt tcctcctgtg gctcgccggg gatgtgctgc
     2881 tcctcctgtt cgcgggcgtg ctcgtcgcga tcttcatcga tggcatcgcg aactggatcc
     2941 gaatccacac ccgcgtcggg cgtggggctt ccatcgcgat cgcgctcctc ggtctcgtgc
     3001 tggtattcag cgtggtgggg atctactccg cgccggacat cgccgatcag gtgggccagc
     3061 tggggcagga gcttccgcgc gcggtggatg cggtccgcca gcaactcgac aagcacgctt
     3121 ggaccaagtt gatcatcgac cagctaccca gcgcgaaaga gctgcggtcc cccggaaccg
     3181 gtctcctgcg acgggcgacc ggggctcttt ccagcacggc gatcgccctc gcgcgcatgg
     3241 tcctgatcgc ggcaatcggc gtcttcctca cgctccagcc gggcttgtat cgccgcggct
     3301 tccttcggct cattcccatt cgaaagcgtg ggcgcgcggc cgacgtgctc gacgcggtca
     3361 cgaacaatct gcgcggctgg ctcgtgggaa aggtgctttc ggcgatcgcg atcggcgtcg
     3421 cgacgtggac cggactccag ctcctcggga ttccgctcgc gctcattctc tccgtgctcg
     3481 ccggagtgtt cagcttcatt cccaacgtcg gtccggtgct cgctgtggtc ccggccatgc
     3541 ttctcggact cctgcagagt ccgtcgctcg ctctctacgt cttcctcctt tacctcggca
     3601 tccagatcgt cgagacctac ctgctgaccc catggctggc acatcgcacc gcggaccttc
     3661 cgcccgcgtt gactctggtg acccagatcc tgctcggcgt gcttttcggg ggcatggggg
     3721 tgatcatggc ggcccccgtc accgcggcgg gcatggtgct gatgcggatg gtctacgtgg
     3781 aggacgtgct cggcgacaag ggggctcagg agaacctgcc cccgcgggat cgagtgggta
     3841 gctcgggcta gtcctcttgg cgggcgcccc agaagcggtg ggccgcctcc acgtagatcg
     3901 cggcgcaacc gaccaccgaa gcgacgtcca cccactccac ggcctcgtgc gggctggcgc
     3961 ccgtgggacc gtagttgacg gtggggatcc cggcctccgc aaagagcgcc gagtccatcc
     4021 agtagggcgc gcccttgtcc cgaggcacct tccccgtcac cgcgcgcaca gcgtcccgca
     4081 ccgagaccgc cagtgggctg tcggcggcgg tgtggctggc tcgacgcacc gtgcggatcg
     4141 agacctcggc ctcctccccc gcttcctgga tcacgcgctt cagctcctct tcgacctgat
     4201 cgccggtctc gccggggagc gttcggcgtt cgatcgagag cgagcagtcg ggagcgtagg
     4261 tcgagagacc gacgccgccg cgcacgagcg cgcagtgcat cgacgccggt cccacgagcg
     4321 gcgcggttcg tgtgcggagc gtgtcggcat cgaagcggtc gagcgcagcg atgatccgcc
     4381 ccatgcgggc aatggcgctc acgcccaggt cgaagcggct tccgtgcgcc gcgcggcctc
     4441 gcgtcgtgat gtcggcccag acgaatccct tgtgcgccag gatcaggtcg ccctcgctgg
     4501 attcggagac gatgcagccg tcggcgcgat ggcgccggac gaagtccgcg gccccgacgc
     4561 tctcgtactc ctcgtccgcg acgagcgcga gcaggacgcg tcccgggagt cgctcgcgcg
     4621 cgagcgcggc ggccgccgcc atgcaggccg ccacccctcc cttcatgtcg gtcgcgccgc
     4681 ggccgtacag acgtcccgcc tcgacgcgcg gctcgaaagg gggaatcgtc atccccgccg
     4741 tcccgaccgt gtcgaggtgt ccgtacagga tcagcgccgg accgccttcg ccgatctcgg
     4801 cggccacgtt gggacgtccc ggggccgcgt cctccttcca ggcgcgaatg ccgttggcca
     4861 ggagccagcc acacgcgaat tcggcgatgg cgccctcgcc gtgcgcttcg tcgggagcga
     4921 tcgcgggatt ggtcgagggg atgcgcacca ggtcggccag cagcgcgacg accgcgccgg
     4981 gagcgagcgg gtcgggaacg gggcagctcg ggatggcagg cgcccgggcc gcggcggcgc
     5041 cggcgacggt cggtttcgcg ctcacgacga cgccgtcgct tccagccagg gacccaggaa
     5101 gtcgagactg acccgccagt tcttctcgag gagcgccaga gcgcgctcca gatcgccccg
     5161 ccggatggcc gtctggatct cgcgatgctg cgtcaccgac gtgatgactt gccccgagtt
     5221 ccgcatgaac gcttcctcgt agcgggccgc gtggtccttc aggttctcga tcatcgaaag
     5281 aagcatccgg ttgggacagc cggcgaggag cagggcgtgc cattcgcggt cgcgcgcgag
     5341 cgccaccgac gcctccgcca gggcctcctc gagacggcgg ttcgcctggt ccagctgccg
     5401 cacgcgcgag ggaggaaggg gccacgacgc gcgcagggca gctccctcca acgtccagag
     5461 aatcgggtag atctcctccg catcgctccg gcggagcgcc cgcacggaga atccccggcc
     5521 cggcgtcgcg gtaagaaagc cctcctgtgt caggccgagc atagcctcgc ggagcggggt
     5581 gcggctcacg ccgagccgcg tcgagagctc ggcttcgttg atcccctccg agggagcgag
     5641 gtcgccgcgc acgatctcgc ccaggagaag ccggcgcacc tcgctcctca ggggggtgcg
     5701 cgtgatggga gggcgtacct gataggcggc gcggctcacg agacggaggc tcccgggctg
     5761 cccgccgcgg cccgctggcg gcaccaggcg tagagagcgt cgtagagcgg cgcctcccgc
     5821 tccagcttcg tgcgatcgtc gcgtccgtgg accagggcaa acccttccgc gatggcccgc
     5881 agtccccgcg cctccggcgc gcgatcgatg tcgtccgcga catcggcggc gtggacgatc
     5941 gccgccacgc cttccaggcc cggatccccc gtgagcccgt acttgagaag gatgctctcg
     6001 aaggagcaac gtccctccac atgcccgagc tcggctcccg gcacgtcgta ggggatcgcc
     6061 gcttcggcgg cggccacggc ggtgacctga tcggcgggca cgaacaggaa gacggcctcg
     6121 ggatcgacga agcggcggat cagccaggga caggcgatcc ggtcgacctt ggcgttttca
     6181 cgggtgatcc atttcatggc ggcctcctgg taacgcgtga gtttcgtatt ttgtatacaa
     6241 aatgctctga ggtcaagacg tcccgtgtcg tgactcaggg accgcgtctt ccctgccctc
     6301 tcagcgaacg gcgggttctc cgctgccggc cagctcccgc gccgaggcgc gcacgaacgc
     6361 gacgaccagc ggatggggcg cgttgggacg cgacttccgc tgaggctgga agagggtggc
     6421 gatgtagaag gggtgttccg gcacttcgta gatccgcgaa tcgccgcggt cgccgaagcc
     6481 cgtgaactcc aggccatgct cttcgaagag ctcgtcgtaa tcggggttca gctcgaagtt
     6541 gcagaagtac tcttcttcga tccggtcggt gccgagaatc tcgcgggcgc gggaatcctc
     6601 ccggatgtgc acgcgctgcg ccggcccgga aagcttgggc gcgtcgtcct cgcggccggg
     6661 aacgggacag gacacctgat ggatcaggag gttctcggct tcgggcgcgt cctcctcgtg
     6721 ttccagatcg gtccgcccaa ggacgttgtg cgcgaactcg agcaggccgt actggaaccc
     6781 ggcgcaggtg gcgagcatcg gccgctttcc ttcgcgcccg taccggatgg cagccagcgc
     6841 gccgggccgg ctcgcgtagg gactgccggc gttgatccac aacccatccc agtcggccag
     6901 caccgattcc ggattgtcgg gagcgatcga cgcggtgccg atccactcga cctcgaccgg
     6961 aacgccgagc ttcgcggccg cgtgctcgat ggccgcgccg gtcgtctggt gcgacacgaa
     7021 gtcggggttg tagtccccga cgatggcgat tcgaatcgga gcagccacgg cgttcctcct
     7081 ctatgacgag acacgtgtca cggtgtcggg ggtcgcgatg cggccgaggc gatgacctcg
     7141 ggtccggaac cggaccagtg tacgacgcgg aaggcgaaaa ggatcgggtt tatcgcggga
     7201 gggatcgggt ttatcgcggg tccccgccgg gggatgcggg cggcccagac ccggttaaaa
     7261 gcccttcccg gccccgagcg cctcgcgcag cgccgccgcc gcgcgcgccc gatggctgat
     7321 cgcgtccttg gtcccgccgg gcagctcggc gaacgttcgc gtctccccgt tcggaacgaa
     7381 cagggggtcg tacccgaacc ctccggtccc ccgcggcgct tccgcgatca gcccttcgca
     7441 caccccttcg accagcgcgc ccggcgcgcc gggagcctcg agcgccagga cgcagcggaa
     7501 ccgcgccgtg cgccgctccg gcggcacgcc gaccaggcgc tcgagcagga gcgcgttgtt
     7561 cgccgcgtcg cccgcggcct cccccgcgta gcgcgccgac cggatcccgg gctccccgcc
     7621 cagcgcgtcg acctcgagcc ccgagtcgtc gccgatcgcg gccacagcca gcgcttcgct
     7681 cacggcccgc gccttggcgc gcgcgttctc ggcaaacgac tcgtgtcctt cgggaggaag
     7741 ggtgacgccg gggtacgcat ccagcgactc cacgcgcgcg gcgatcccct ggagcagggt
     7801 ctggagctcg cgcgccttgc cggcatttcg ggtcgcgagg acgacccgct cgatcacgcc
     7861 caggtcttcg gcagggtccg gatgccgagc accttcttct gctcggcaat gagccggcgg
     7921 atgccggcct tgcccaggcc gatcagctgc gccagctccc gctcgctgaa cgcttcgccc
     7981 tcggccgtcc cctggatctc cacgagacga cccccgccgg tcatgaccag gttcatgtcg
     8041 acctgcgctt tggagtcttc ggcgtagcag aggtcgagcg agggcgtgcc gttcacgagc
     8101 ccgaccgaga cggcggccac gaagtcgcgc agcggcggtc ccggaagctt cacccgcgcg
     8161 gagagccacc ggatcgcgtc ggccagcgcg atgaaagctc cggtgatcga ggcggtccgc
     8221 gtgccgccgt ccgcctcgat gacatcgcag tcgatcagga tctgccgttc gcccagcgcc
     8281 tcgagattcg cgaccgcacg gagcgaacgt ccgatgagcc gctggatctc ctgcgtgcgg
     8341 ccgcccacgt ggccccggtt cacttcccgc tggatccggt catgggagga gcgcggcagc
     8401 atgctgtact cggcggtgat ccacccccgg cccccgcctc gcagccaggg gggcacgcgt
     8461 tcttcgacgg tcgccgcgca gagaacgcgc gtcccgccca tctcgatcag cgccgatcct
     8521 tcggcgttct tgagatagcc gcgccggatc gacacggggc ggagctcggc ggcgcggcga
     8581 ccatcgacgc ggcgacctgg acgcggcgct tgccgcgtgg cctggcgcga ctcctgacgc
     8641 gcggggctca tcgcgcccag ctcgccgggc gttcgaacca cggcagatca tcttgaggca
     8701 cgttgcgcac gcggcccatc gccttgccga gaaaccgccg gcccaccagc tcgaactgct
     8761 gcggaatgtc ggagacgaag cattcgagcg agccgcgccc atccgcgcgc gccacccgcg
     8821 ccaacgcccg cgccgccttc ccgccgcgcg ccgagcgtcc ggccgcgcgc gcttcggccc
     8881 gcaccgcggc cgcaaggcgc cgagccgtcg cctctcccga atcgatgagc cggacgcgcc
     8941 cgcccagcac cgcgcggatc gcgcgcttga acacggggta gtgcgtgcac ccgaggatca
     9001 gcgtgtcgat gcggcgacgc gcgagcgggg ccaggtattc gcgcagcact tcgcgcgcgc
     9061 ccttcgagtt ctgcaatccc tcctcggcga acggcacgag cagcggacag ggctcggccc
     9121 agacgcgcgt gcgcacgccg gcgcggcggg aggccgccgc gatcgcgtgc tgataggccg
     9181 cgctcgccac cgtggcgcgc gttccaatca cgccgatccg tccgttcttc gtggcgcgca
     9241 cggcggcatc ggcaccgggc acgatgactc cggtcacggg aagcccctgg aactcgcgct
     9301 ccaggtgacg cagcgcgacg gccgacacgg tattgcacgc gaccacgatg cggctcacgt
     9361 gaaactggga gaggaagcgc accgcctcgg aggcgaagcg gcggacggtc gcgtccgact
     9421 tggcgccgta ggggacacgc gcggtgtcgc cgaagtagac gatctcccat gacggaagga
     9481 gccggcgcag atggcgcacg acggtgaggc cgccgatgcc ggagtcgaac acgccgagcc
     9541 gcacgctagc gccagtcctc ggcttcgagc ggctgcaggg tgtcgaagtg cccgccgatg
     9601 gtctcgatcg ggttcccctc gacgagaagg gtgacgcgct gcacctcggg caggttctcc
     9661 acggcgctct tcacgatcga gcccaggagc tggcgctcgc gtccggtgcc gccgcggaat
     9721 cccgcgacca gcgcccggtt ccagtccagg tagagcgtcc cgcgctgcgt ccagtaggta
     9781 ttgagaagct cggtgccgtc ggggaagggc gagacgcggt ttccggatgg gccggcgatc
     9841 agctcgcgga agatggcgcg cacctggtcc acggggtggt ccttcacgac gacgtcgcga
     9901 tactcggcga cgagactgtc cgaattggga agcgcgtaat agaggtagac gccgcgcacg
     9961 ccctccagct ccgcggcgac gggcgaatag cgggagagcc ccagctcgtt ccgccgccag
    10021 ggcgctttcc agatcaggag ggcgatggcg atccccacca aagcggcgat cccgcccacg
    10081 atccatcggc ggcgcgcgac ggcgcccggc cccgggcttc cgaacgtttc gctcatgctc
    10141 tcgattttcg cacgaagtcc tcgatcgcgc gcgcgacggt ctgggtgtag tcgtaggtcg
    10201 tgtagccgcg catgctcatc gactcgggac cggaccgggc ggcgggggcc gactccacca
    10261 cgatcgccgg gcagagtacc ggctccaaca cgctcatccc gccggtccgg acgagcaccg
    10321 gagcctgcgg gaaggagcgc gcaagcgcgt cggccacggt ctgggcgaag gcgatgctcg
    10381 cccccaccac cgccgactgc gccgaccgcc acggcttgag ctcgatcccg tcgccgtccc
    10441 ccggcaggac gaccggaacg ccgccccctt cgttcggcga agcgaccggg gactcggcga
    10501 ggagccggaa gccgcctccc ttgatcgttc cgtcgccctc caggtggatg ctgatgaaga
    10561 gatccgcccc ggcctcgttc gccagctcgg cgcgccgcgc cggcgagagg aggacgtcgc
    10621 tttcgcgcgt gagcacgatc ttcgcgttca agcgcgacgc gagcgcggtg cgcagcgagc
    10681 gcgcgatctc gagcgtggcg tccttctccg ccatcccgcc cgggagctgg gcgccgaggt
    10741 cggtgccgcc gtggcccgga tcgatcacga tcgtccgcac ggcgttcgcc ttgcccgcga
    10801 cccgcagcgg ctcgagcggc gtgaagagcc cggcctcgac cagcccgggg tcgtcggtca
    10861 ccgccacctt catgcggttg ggagcgctcg aaacctgata gccgaccgcg ctgtcgccga
    10921 ggaccaggcg caggtccacg ccgcccggga tctcgcggat ctctccgcgc gcgaccgcgc
    10981 ctccggtgaa gacgtgcgcc gtgtcgaggg tccccccttc gaagagcacc cgcacctcgc
    11041 ccggcatcgc gtagaggaga cgcgcgggaa ggcttcggcc caacgtcgcc gagacttcgg
    11101 tcacccggcc gcgcacgaac agctgcgcct ggcgcacgga gtgcacgacg ccccggaagc
    11161 ggatcgttcg cccggtctcg tcccaggcgg cgtcgctagc caggccccag tcgaagatgc
    11221 gctcgacgat gtcctccggg gcgtagacca cgccgcgcac gagggtcgcg ggccggatca
    11281 ggttcttcgc cgcctcgtcc acgagcacca ccggcgcccc gatcgagaac cgcagcgtgt
    11341 gctcgccgat ccggagcgag agcttctgga tctccggccg ccagtacttg gtcgcgccga
    11401 agacgcgggc caggtcgttc gtcgagacgt agcggacgcc gtccaggacg atcgggacga
    11461 agcgctcggg attgcgcggc tcgggataga tgaccgtgat cggcgccgcc gagcattcgg
    11521 cgcgggtcag cgcgggaacg agcgccgcga gaagaagcag gaggagcagg aggagggaga
    11581 cccgggtcgg aacgtcgcgc atcagtccgg gcgctccccg cgcatgaccc cgcgcaattc
    11641 ccggcgcgag cggcgctcgt ccagcttcgc ccgcttgtcg aattcgcgcc gtccgcgcac
    11701 cagggccagc tccaccttca cctttccgtt cttgaaatag agcgagagcg gcaccagcgt
    11761 gagtcccgac tcctcgagct tcgtcgtcag ccggcggatc tccgcccgat gcatgaggag
    11821 ccgacgcgcg cgcgtcgggt cgtgattttc gtgcgccgcg ggatcgtacg gattgatgtg
    11881 ggcgccgcgc aggaagagct gcccgccttc gatggtggcg taggcgtcct ggagctgggc
    11941 cttggcgtcg cgcagcgact tgacctcgct cccccggagc tcgatgccgg tctcgtaggt
    12001 ctcgatgacc cagtagtcgt gacgagcctt ccggttcgtc gcgatcacgc gccgctcgtc
    12061 ctcgcgcttg ggcttgtcct gtttgggctt ggaatcgtgg tcgcgcgcca tcccgtcgtc
    12121 tctccccctc ggtctgttgc ttgtaccatg cgggggtcgg tggcgcaatg agggcgggga
    12181 agcctcgatt gacgggccga agcgtagctc gttatactgt cccgctctac aacgcccgca
    12241 tcttcggtgc ggaagtggcg gaactggcag acgcgctggg ttcagggtcc agtgccttca
    12301 cgggcgtggg ggttcaaatc cccccttccg caccatgttt tcgacaaggc cgctcccttc
    12361 ccgccaccag gggccggctc cccctgatgc tcccgcgatt gtgccacgct cctgcttgcg
    12421 ctagtgtcgc tctactcccc gcaaccatcg aggcttccgt gatcgaatcc atccgaagca
    12481 cgcggcggtg gaagcgatgt ggcctcgcgg tcacctctat cgcggggatg ctgctgcatt
    12541 ccgctttctc gttcgccgcc ccgcctctca ccttccgttg gccggtaccc tgccgcgtgg
    12601 gagtgaccac gatcgcccag accgacacta cgaccatggt gatccatgag gtagagcacc
    12661 tgtacgcgac acctcatccg ggagagctca agctgcagag cgaagagccc tttgtcgccg
    12721 attttgtgat cggatcgtcg cggcccccgc gttcgatcat cgacgacctc atggcgttgg
    12781 cgagcaatga tcctcccatg accatcagtc gtgacggcgc gttcctggga tgtcatttca
    12841 gcgacgagta ccttgctcag gtccaatcct ggatccgcga caagatcggc cccgttccgg
    12901 acacgacact gctgaagcag gtcctcgagc ttcagcgaag tccggagatg cgcgccaagc
    12961 tggaggcgaa gagcggcgag gtctggaatg cctgggtggg ccattggatc gaatcaacgc
    13021 tcgacccggg gatgcaggtc gatgcccgga tgccgttgcc ttggatctcg ggcacgatcg
    13081 agagcgacgt tcaattccag aaccacggcc ccacccggac cggagggagg ctggatcgcc
    13141 ttgtctgggt gacccgtacc acgggggagg aggcgcgcct agcctacgag cgcgccgtgc
    13201 gcgagaccct gtcgaaaatg cccgaccttg gcactccgcc ccggacaccc ctgctcggcg
    13261 tgatccgaac cacctccctc gaaatcgtcc tggaccccgt gtcgatgcag ccgaactacg
    13321 tgcacgagga gacgaagctc gatctgcagg actctgtggc cgtgcacagc agccaggaag
    13381 cgaaatccta cttcttcgag tgggacccgc cactcgccgc ggccctggac ccggtccctc
    13441 cggaggggat ttccgccttt ctcgaaaacc gcctcccgga cgcctatttc gggctccgca
    13501 cggacgtgga aaatggaacg cgcaatcccg acacctacgc ctacctggcg gagacctgcc
    13561 gcaggctggg tcagttcggg gaaggtgttc tcttcgcccg aagggccctc gccctgaatt
    13621 cgcgccatgc cttcgcgcac gaagttctcg ggaactgcta caacggccag tacggaccgt
    13681 ggccgctcgc gagccgggat tccacatgga cgcatctcct gctcgcggtc gagtcggact
    13741 cgaccgacgg caacgcgtgg ctcggcgtat ttctcgaatc cagccggcgg ggtcgcaacg
    13801 acctggaggg gcgctcgctg tcgaaactcc tcgaaacggg gttcctgacg cgccccattc
    13861 tcgctcgcgc ccggtggatg ctgcaggatc tgcccgacag cgccgtgctc gtgacgaacg
    13921 gagatatgga cagctatccg ctctgggcgc tccagatgag ggaccgcctg cggccggacg
    13981 tggcggtggt caaccgatcg cttctcgagg cgccgtggta cgcgcgcttg gtgcgggacc
    14041 ggctgcgcgt cccgcttccc tttggggaca gtgtgctgga ctctctcacc cctgagggcg
    14101 ccgactcgaa cgtcgtgtac gtctccgatc ggatcatccg gggatggctg gaccagcagg
    14161 cctcggggat cttccaaagg ccgcttgcct ttgccttcac catacccgaa gggtggttct
    14221 cgccgagttg cgccgcgcga tcggcgctat cggggccgtt ctatctctgt cgggcgacgc
    14281 cggcgccctc ccccctcgac acgaccgcga tccgaatcag cctcgggcat gcgcgggcat
    14341 ccgatttcgc cggcccgtac ttctccgagc gggatcggag ccccgttcga agggccgccg
    14401 tgtcgcccgc ggaagacgtc gtcgagatgt ggttccgtta cgctgacgcg ctgggtcgtg
    14461 ccggacgccg gagggactgc cttcgggctc tcgagaagac ctcggccttc gcccgcgagg
    14521 cgggcacgtc acccgcgtgg cgtgcgatga tggagaagat gcgggaccgg tacagccact
    14581 gaggacgcca aaccatcgag gaagttaccc atggacaaca cgacgctcac gctggaccag
    14641 ctccgcgacg cggggcggaa cgtctcggcc gccgatcacc cgggaacgat ccgcgcctac
    14701 taccccttct gggacaccca gtaccggccg tttctcctcg cggcggtcgc ggcgctgccc
    14761 cgcgagaagt ttgacttcaa gccgcgcccg gagatgttca cggcgcacga gatgatcgtc
    14821 cacatcgccg agtgcgagcg gcattggatc cacgtcgtgg tcgagggcgg agcgtacgag
    14881 gagtgggtcg ccccggccga ggacccgaag caggggtgga tgaacgtcct tgatctgccg
    14941 gatcacgcgt cgctcttcgc ggcgctggag aagtggcacc ggaacacgca gcagcagctc
    15001 gaacgcccgg cgtcggatct gtcccggcgg atcacttggc gcgctccctc gggccgggag
    15061 cacacctata ccctgcactg gatcctggac catctgcagg agcacgagat ccatcatcgc
    15121 gggcagctca acatgtatct ccggatgatg gggatcgagc cgccctcgat ctgaaacggc
    15181 gcggacgccg cgcgaatcta cgagtgcagc ggcttgtgcg accgcttgcg aaatccccgc
    15241 gagagcacgc ccaggatcgc cagcgccgcc gcgaacgcgc cgcccgcgat cttaggctcg
    15301 atcttgcccg cgagcgtcat cacggcgaga agcatgcaga tcatcaccag cagccatatc
    15361 gcgtccttac cttccatcga actctcctcc gcacggatac cggccgaggg ccggccgaac
    15421 gtcaggccgg tcgagagggg gtggggaaac ccctgctttt cacactagcc gccccacccc
    15481 gctctcgaaa tggtccgctg gtacacctgc cgccctaccg cacgagcacg aagcgccgcg
    15541 tgatggtgtg ccccggcgcg cggaaccgca cgaaatagac ccctgaggga gcctggccgt
    15601 gcggggtgcg gccgccccag cggacggagt gacgccccat gggatacgcg gcgtccgcca
    15661 gcgtctcgac gaggcggccg gccgcgtcga acagctccac gcgaacccgc tccgtgcgag
    15721 gcagctcgaa cgcgagcgag gccccgttcg acgtcggatt ggaggatgtc atgcgcatcg
    15781 agaaggccgt gaccggctgg tcgtccacgc ccgtgaccag atcggggatc gtgaacgcag
    15841 cgtcgctcag gtcgaagccc gagagcgacg gactcagcac ggccgggttg tgcgccacca
    15901 ccttgacgta cccattggcc gttcccggat aggagacgtt ccagaggaac gatccggtgt
    15961 tctcgatccc ctccccgagc gtctcgaagg gacccgaagg cccgttccgg gacaggagca
    16021 cgtcgaccag ctccacgccc tcggcgtcct cggccgacca gcggatctcg tagaggctcc
    16081 cgatggccag cgcctcgccg ccgttcggcg cgctcaccgc gaccagcggc gcgccgatct
    16141 cgttgaagat cgcggtcacc cgcttgtccc ggctcatggt gagcgatatc gggttggccg
    16201 acccggcggc gtcgccgcac cagctccgga actcccatcc cgaggccggg gaggccgtga
    16261 gctggacgac cgatccctcg tcgtaggccg cgcggttggg attcggcgtc accgccccgc
    16321 ctgccgcggg cgccgagacg accgcgagcg tgtgcgtcgg cacgacctgc gtcgtgaatc
    16381 cccaggtcgg tccggtcacg gtcgccacgc cgtcgctcac cgtcgtgtac cactgatacg
    16441 gcatccctcc ggcgagtccg ctccacacca ggctggccgt gccgcccgac gcgacgcccg
    16501 ccgacgtgcc caggagggcg tacggctcgg cggcgctcat cgagtacgga acgtcgaact
    16561 ggctgtcggc gtcgttctcg gtctggttca gccagggcga ataggtgctg acgtggatca
    16621 cgttgttcgc gggcgagaac tgcatgatgc ggagccaccc gtttccgccg ttggtgcgcc
    16681 cctggtaatc ggacatcagc gtgtacaccg tgttgccgtt gaaggtgtcg gagcgccgtc
    16741 cttcgcccgg cacgtgcccg cagagcatca ggaacagatt ggaatgggtc ttcagacggt
    16801 cgtacgtggc ctgtccctgc gcgctccagg cgcccggatt gccggtcccg atgaagttgt
    16861 gcgagaggac gatcgcgcgc cggttcgcgt aggtcgtgag gagaccgtcg gcccagtcga
    16921 ggatcgccgc gtcgggcgtc gtgtcgtatt cgagcgagat caccacgaag tccatcccgc
    16981 cggcgcggaa caggttgaac cagttgtcgt tgttcgaccc gtagtgcccg ccgtagtagg
    17041 tccttcccgt gaagcggttc gcgccgaagt actggttgta gaagaccgtc gtggccccgt
    17101 ccgggttgcc gttcggagac tgatcgtggt tccccaccgt gatcccgtac ggaagtccct
    17161 ccgggagtcc cgtggtcagc ggattctcga tcagggagta gccggcgtcg gcgtgctgcc
    17221 actcgatgtc gttgccgccg ttgtcgccgt gttccacgca gtcgccaagc gtcgcgacgt
    17281 atccgatgtt ccgcgacgcg cggttcgccg tgatccagtt cgtctgcgag aggaggatgg
    17341 cgttggtgcc gccgttcagc tcgcccgtgt agtactgcgt gtcgggcatg ccgatgatcg
    17401 tgaagtcggt gcccggcgag gagaccgccg agcgtccata gaactggacc gtcaggctct
    17461 ggccgtcggg atcggcggcc gagacctgga gcgtgggcga cagcggcacg tcggtcgcct
    17521 ggtccgccgg tccgacgagc gccggagcgc taggcggcag gttgaacgcc aggttgaacg
    17581 gagcgccgga aacccacgcg tacgcggtgc cggtgatcgt cccattcacc gaggtgcccg
    17641 ccgagccgcc cacggccgtg ccggagcctt cgtccagcgc ccagcgcgcg accagaccgg
    17701 ccgccggagt ggagatctgc gcgttgatgg tcccctggat atccgactgg gtccgcgccg
    17761 cgttccagat ccg
//
LOCUS       DASSLZ010000213        19751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_836417, whole genome shotgun sequence.
ACCESSION   DASSLZ010000213 DASSLZ010000000
VERSION     DASSLZ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 19751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19751
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836417"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /gene="yidC"
                     /locus_tag="VFM00_08195"
     CDS             <1..1235
                     /gene="yidC"
                     /locus_tag="VFM00_08195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET7498266.1"
                     /translation="SYDFTGAPFRKTGGSAREGWLAYVAEDSSGVRVTKTYRLSPDAH
                     LLDVEVRIAAPAALGPIHYQMGWGNPLPLTELNARPLERTAVAYLGAKLETVDANRIA
                     KDVTKRIAGNVRWAGERSKYFIATVIPDSATIPEVLFQQGPDKLPIVWLSGDAAPGTE
                     IVRHGRIYAGPIHFETLVQIGAGLEEVANLGWKWIVPVSAVLLKMLIAIHHAIPNYGI
                     AIIIVALAAKLVFYPLTQSSLRTMKVMHRLQPEVNALREKHASDPAKMNTAMMNLYRE
                     HKVNPMGGCLPMLLQLPVFLALYQVLLHAIELRSAGFGWWIKDLSAPDVVAYLGGFPL
                     HVLPLIMTGSTFLLQSQTPVDPRQQFMMYLMPVMMLYIMYNLPSGVIIYWTVNNLVSA
                     LQQYLVNVAEDRRMAAHT"
     gene            1271..2101
                     /locus_tag="VFM00_08200"
     CDS             1271..2101
                     /locus_tag="VFM00_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HET7498267.1"
                     /translation="MNDSDDIRVEGETFQDAVAAAAAQLGLKTTELGIEIIDPGASAD
                     SSTGYRPVRIRAWRRDPESSADGLRKPAPGARRAPSDYDDGGRRSHHGPPRRPAPVNY
                     GPPPPPMDPEKITPELVTMVRELADGIMKHMEFPSTVEAEKTKHGIRVAIQAGDWDQY
                     LIGPDGETLAALQHIIGRMIRAKMPDEAPPRLEVDVAGYRDRQIEALREMARGLIAEA
                     KDSGEEVTTDPLPASERRIVHLEVAEVAGMETVTIGDGFYKRVVIRRASADAAAKGDR
                     "
     gene            2108..3511
                     /gene="mnmE"
                     /locus_tag="VFM00_08205"
     CDS             2108..3511
                     /gene="mnmE"
                     /locus_tag="VFM00_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET7498268.1"
                     /translation="MADDPGDTIVAPATAPGEAAVALIRVSGPAALGLAARHFRGSRS
                     PEESPSHHILFGQFVDGRGDPVDTVLVSIFRAPRSYTGEDVVEISSHGGAVVPAAILD
                     TLIRSGARPARPGEFTERAFRNGKLDLAQAEAVAALVRSRSDSAARAAGATLGGALSR
                     RVDRLDAALVALLAEVEARIDFPADAGEPLDAVALARRCGALASEIEEWVQGLPGARR
                     RESGLSVVLAGPPNVGKSSLLNALVGYDRAIVSEIPGTTRDTVESSIWIEGIELRLTD
                     TAGLRESHDPVERLGIERTASALERTDLAVVVLDRSDPEASVTALGSSGSPSRPVVVA
                     WNKADLARGRAAMGGRWAGTPLEGRTVLAEVETVAIAPAGAAPLRNALRDSLPSILGG
                     AAGDEVPATSARQEALLADALAALRRAEAGLAADESYDLIAVDLTEARRSLGEMVGRG
                     VDQGVIAAVFARFCIGK"
     gene            3508..5355
                     /gene="mnmG"
                     /locus_tag="VFM00_08210"
     CDS             3508..5355
                     /gene="mnmG"
                     /locus_tag="VFM00_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET7498269.1"
                     /translation="MSGGAAVEHWEGSGAGGNGAGARTRFPILVIGAGHAGCEAACIA
                     AKMGVEVGLVTMSLDSIAHMPCNPAIGGLAKGHLVREIDALGGIMGRMADRAGIQFKM
                     LNRGRGPAVWSPRAQEDKALYRAIVREHLGRTPAITLVQAQVVGLLVADGMVRGALTA
                     DGRTLEAQAIVVTPGTFLNGLLHIGDQSMPGGRVGEAPARGISDCLVDLGFSLVRLKT
                     GTPPRIHRDSIDFSRCVEQPGDDPPRPFSHFTGALQVDQVLCHLTETNAATHDLIRAN
                     LHRSPLYTGKIRGIGPRYCPSVEDKVVRFAEKPAHQIFLEPEGRSTHEYYVNGLSTSL
                     PEDVQRAMLKTIPGLEDAELLRTGYAVEYDFVPPTQLRATLETRPVRGLYLAGQINGT
                     SGYEEAAAQGLMAGINAASRLVGRDPFVLRRDEAYIGVLIDDLVTKGTEEPYRMFTSS
                     AEHRLLLRQDNAPERLAHHARALGTLASDELDALDARVVARRDAVSRLRAVRGSGSIT
                     NRPHETLPVMDEIQESALIEIRYEGYIERQRRTVERASRNESLGIPEDMIGQELKGLS
                     REAHEKIARFKPSTVGQASRLPGISPSDVAVLTIYAERERRRGPRATPR"
     gene            5309..6004
                     /gene="rsmG"
                     /locus_tag="VFM00_08215"
     CDS             5309..6004
                     /gene="rsmG"
                     /locus_tag="VFM00_08215"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET7498270.1"
                     /translation="MPNESAGADPAPLPAELIPDFERFRSELSAVSREAAWAGLFGDP
                     RAEAGWREYGRLLHERSAALALIAKGDRGEVFTRHILDSLNPVALFDTPPASLLDVGS
                     GGGLPGIPLAIAWPDARALLLESRERKAGFLELASRQLGLRHVRVACTRLEDLESQWR
                     EERVEAVFVRALGDLDRVLGLAAAVARPGARWVYFLGMRASGEALPSGSPFDPRIVEG
                     VFGGRLLTGVFPG"
     gene            7020..7781
                     /locus_tag="VFM00_08220"
     CDS             7020..7781
                     /locus_tag="VFM00_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET7498271.1"
                     /translation="MPRTVAVANQKGGVGKTTTAVNLAAALAMAQRRVLLIDLDPQGN
                     AGSGLGYPGVKVTRGIYDVLTSHATLREVLMETEVPGLSLVPSGQRLVGAEVELVGVD
                     RREYRLKDALREIEGDFDFVLIDAPPSLGFLTINALTAARSVLVPVQCEYYALEGLSH
                     LLGAIRLVQQGLNPELQIEGVLLTMYDNRLNLSQQVLEETRRFFAERVYRTVIPRNVR
                     LSEAPSFGKPVVIYDPQCVGATSYVNLAREVLERG"
     gene            7837..8700
                     /locus_tag="VFM00_08225"
     CDS             7837..8700
                     /locus_tag="VFM00_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET7498272.1"
                     /translation="MPIIPESRPPEARAAEARPGDVQHIPLAKIVRNPSQPRTQFDPS
                     AIQELAASIRERGVLQPVLLRPAGDGYQLVAGERRFLAAREAGFASIPAIIRPLTDRE
                     SLLIALIENIQRENLNPIDEARAYYRLATEYGLQHEEIGQRVGKDRSTVSNAIRFNNL
                     PADVQSLMESGALSAGHGRALLSLSTSKQQSDLGASAASHAWSVRDLEERIRRSGAPR
                     AAKARRSPRRAFSAELMALEDELMRVMGAKVRLTRRRGGSGSILIEYHNEEELERILG
                     MLRDISPAGHA"
     gene            8982..9998
                     /locus_tag="VFM00_08230"
     CDS             8982..9998
                     /locus_tag="VFM00_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7498273.1"
                     /translation="MTHSTASGDVRLLEELSTARGEILTELRKVIVGQDVVVEQLLTV
                     LFANGHCLIVGVPGLAKTLLVSTLATILDLQFSRIQFTPDLMPSDITGTEILEEEAGT
                     GHRSFKFIHGPIFANVVLADEINRTPPKTQAALLQAMQEYRVTAGGTTYPLSLPFFVL
                     ATQNPIEFEGTYPLPEAQLDRFMFHISVGYPSDVEEERIVTNTTSAYEAHPKKVLSVE
                     RIIDLQRLVRRIPVSEHVVRYAVRLARASRPGENAAPFVKDWVSWGAGPRAAQYLVLG
                     AKARAALAGRPTPSADDVKAVAPAVLRHRIIPNFTAEADGISTLDIIAKLLEATPSVD
                     GKHA"
     gene            10068..10976
                     /locus_tag="VFM00_08235"
     CDS             10068..10976
                     /locus_tag="VFM00_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7498274.1"
                     /translation="MSPERARERTRLLDPHFVSKLARLDLKARLVVEGFLTGLHRSPY
                     HGFSVEFAEHRQYMPGDPLRHLDWKVLAKSDRKYLKQYEEETNLRASLLVDTSASMGY
                     GSHGITKLDYARQLAAALAYLMLTQNDAVGMFAFAAGRGEHIPARSTMGHLRPLLVLL
                     DRLQPGGATDFAASLHSLAERLTRRGLVVILSDLLDEPERIAQAIHHFRHRKHEVLVF
                     HILDPMEVSFDFQREAVYVDLESGERVTTRPRELRSDYRQRVSEWRETLRQACIEKRA
                     DYVPLTTDQPYDRALLEYLSKRARLL"
     gene            10990..13098
                     /locus_tag="VFM00_08240"
     CDS             10990..13098
                     /locus_tag="VFM00_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HET7498275.1"
                     /translation="MPTLSFLNPAFLWALPAASLPILIHLLSRQRLPEVRFPTTQFLR
                     QLEPREIRRLRLREILLLILRTLALLLSVLAFARPSLAPRGAVTHAAAAVGILLDDSE
                     SMAALDDRARPRMEGAVARALAIADAGRAGDELYLARATRPGEAEIGRARDRVRLRRS
                     IERIEAEPLPARLESSLHSLRLALGRSPLKARELYVISDLQRASLTPEARREITEAAA
                     AGIRVILLPVSEGRTPNHAFTAVDPVTRPGPEGRGLEVRARLVNYADGPSDRLALRVR
                     RGEALVGGGDAALKGGESRWVSMPLEPGAVNDPAIPVVAESDEDALPLDDRWYAVLGA
                     PRRLRVLRIAEPRGAAPAPRFAALALDPSGDGTSGFTVETAGPSALLGLSKSRADVVV
                     LEDVASLSADAEARLKGYVKEGGGLVVALGPHADPEYYTKRLFPGLVDLTLAGPDRSA
                     EGTSFELRARLPGHPILEGLALGVGSPLTQARLSGMMRSRTASARAEVVVQTTGGLPL
                     VVAAPSVAVFLSSFADDWGDLPYSGAFVPLVRGLVSYAARASESEPGSEPRVGERPFA
                     RLEAAPSGAVVARGPAGFTSPAAVENEGAAFRAVADAPAMAAGFYTFEAGGKALATVA
                     LNPDPVESDLAAVPADSLKADARGPNGAPVLSIDNRSALATRLGDTRRGRELWLPLLV
                     LAGVFLIGEILLGSARVLDR"
     gene            13103..16588
                     /gene="mfd"
                     /locus_tag="VFM00_08245"
     CDS             13103..16588
                     /gene="mfd"
                     /locus_tag="VFM00_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET7498276.1"
                     /translation="MNAVREPSRETNPGDPSPVAARLLEAGGTHPRIHELETRLDAAA
                     PGASIRLRGVAGSAGALVAALLARRPGTRLAVIAPDLDRAEGWHDDLCFFLGPDRVVF
                     IPPHDTVSWSAQVATAPVREDRIHAFLRLGDPDPPVAVIPALALHRLAPAPDRIRARA
                     ITVRAGLSIAPEALLRRLVLAGYRSVPEIGESGEVSRRGGIVDVFGPGLDHPVRLEFD
                     GDTVASLRSFDVTSQRSTGPIEEARLAPARELLYDEDVDARLRPLDAPEAPRTAETDR
                     IRDLVAEGIYFEGMEWAAPLLGLPLGSVLDHLAPNTILWVDEPAAVERELEDAERETE
                     RLEEDARVKTPHLPPREALFDSPERAAGRLATRRRVEAALTTREGASRGEAIALDARP
                     QPSFGRKLDLLRTELKRLSDLGMTRMILCDNRGQAERLEELLGGDLGGIDIGDLSAGF
                     VLPGAKLAVFTDHEIFARYRRRRARKLRPSAAALRELLTLTPGDYVVHLDHGIGVFRG
                     LKPLTLDGQETECMQIDYAGGDRLFVPIGQLGMVERYSAEEGRAPAVHRLGGTTWARV
                     KAKTRKAIADMAETLLRHYAVRKARPGRAFGPDTPWQRELESSFIYEETPDQLRAIEE
                     VKKDMEAPRPMDRLICGDVGYGKTEVAIRAAFKAIQDGTQVAVLVPTTVLAQQHFTTF
                     SERYADYPVRVDMLSRFRSPKEQKETVAKIASGEVDLVIGTHRLLSKDVVFKNLGLVV
                     IDEEQRFGVAQKERIRTLVETVDVLTLTATPIPRTLHMSLLGARDMSVMTTPPRGRYP
                     IRTEIVEMSQDVVREALLRESDRGGQSFFVHNRVESIDRIAHYVTSVVPQVKVVVAHG
                     QMKDTQLEKVMVEFLERRADVLVSTMIIESGLDIPTVNTMLVHRADALGLAQLYQLRG
                     RIGRSHHQAFCHLLVPAGKVLTEEAEKRLRVIAEHEELGAGLMIAMRDLEIRGAGNLL
                     GPEQHGHMLSVGFDLYCRMVDEVVHELQGEPVDRRPEPEVSSPLPAFVPDEYVEDRDE
                     KLDVYRRMAALSTLEALEALRAELRDRFGPAPVEVEHLLALKGLRILGKEAGADRLRI
                     GRDRIEIELREPLPRERIMALVPEVPVPIEFASGARTLRIRKPGDPVPLATKVLRALA
                     PVDSVARLPLPAAGR"
     gene            16659..18485
                     /locus_tag="VFM00_08250"
     CDS             16659..18485
                     /locus_tag="VFM00_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET7498277.1"
                     /translation="MIPETEKSTPRRSLGPSRTAAARAAAALLALIALAVPAPGPARA
                     AAPPSARGPIATVGDRTVDALDIQSAALVLGQDPLRKTNPALWRRMLLDRCVDRELLA
                     MEAEKRGIGRDPAIRQRIAEREYAALFQAVYQRVLVPTLDVTPAQMDSLQKTGLYRMV
                     DLYYILLRDDAAQGRRKEAETIVERLRAGGRFDSVAVTKSGHPSRGNGGHFGAVLARD
                     LDPSSYAASRTAKPGDILGPYSGPYGHEIYKVGGFQTLSPDSIRAIVRVERTRNVIRD
                     YQARLLAQYHFLLEPEVVRPILFAMASETPDSLLASMRPDGTRPRMGARPEIGVIARV
                     DGDSLTLPDLLRETHPAPGANGRIRIRDGDALRDLASEALFRRLLVRDAKERGLASDP
                     RVARELRLIREGTAVTAMIAAERPADPGTPELRAWFEAHAARYRRPAARRARVAVFAS
                     QDSAMAALRSWNGVGISDSSLQALRFEEQPRATESTLYPQHAATLPFFDADSDPLGIA
                     VRHLDPGMTSPVVRTAQGYAIAHVLAREPARPMTLDEAIDRVRRDWREEKENEWVLSQ
                     LERMRAQTPVRVVPSRLQAVKLSPAPAATGSLGAVAGNEGGR"
     gene            18482..>19751
                     /locus_tag="VFM00_08255"
     CDS             18482..>19751
                     /locus_tag="VFM00_08255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7498278.1"
                     /translation="MIRRSLIPALAFAALAAAALAGGPTGAAERIAAIVNKSVILSSD
                     VDDQTQQAAARFNVDPSDSASMKKLRKEVLDQLVEKQVILAEATRLGVVVPPKDVNEA
                     VNQEIEQLKERLGGAQAFQAALTQEKTTEAALRKRYEPDVKDQLVIMRTVGREVQNKV
                     TVTDAEVRAYYQANRDSIAKKPESLKLAHILVAFEPDSQQVRRARVRADSIRNVLAKP
                     GKAATFADMAAQLSDDPSGRRGGDLGTFGKGDMVPEFEEVAFALKPMEISNPVRTRFG
                     YHVIQVLEHFPATDSTEERVHARHIMIATKPSPADEERARTRALALRDSLTHGADFGT
                     LARKYSADVATKDSSGYLGEIPVPSLPPNMREVLTALREGEISTPFKRDAGYHIFKVL
                     GRSPETEYKYEDIKDDLKKVVTNRKLEEAYR"
BASE COUNT         3032 a         6964 c         6790 g         2965 t
ORIGIN      
        1 tttcctacga cttcaccggc gcgccgttcc ggaagacggg agggagcgcg cgggagggct
       61 ggctggccta cgtcgccgag gactcgagcg gcgtgcgcgt gacgaagacc taccgcctct
      121 ctccggacgc gcacctgctc gacgtggaag tgcgcatcgc ggcgccggcc gcgctgggac
      181 cgatccacta ccagatggga tggggcaatc cgctcccgct caccgagctg aacgcgaggc
      241 cgctggagcg caccgcggtg gcctatctgg gcgcgaagct cgagacggtg gacgcgaacc
      301 ggatcgccaa ggacgtgacg aagcggatcg cggggaacgt ccgatgggcg ggggagcgca
      361 gcaagtactt catcgcgacc gtgattcccg actccgccac gatccccgag gtgctcttcc
      421 agcaggggcc ggacaagctg ccgatcgtct ggctgtcggg ggatgcggcg cccgggaccg
      481 agatcgtgcg ccacggacgg atctacgcgg ggccgatcca cttcgagacg ctggtgcaga
      541 tcggcgccgg actcgaggaa gtggcgaacc tcgggtggaa gtggatcgtg cccgtgagcg
      601 cggtgctcct caagatgctc atcgcgatcc accacgcgat ccccaactac ggcatcgcca
      661 tcatcatcgt ggcgctcgcg gcgaagctgg tcttctaccc gctcacgcag tccagcctgc
      721 gcacgatgaa agtgatgcat cgcctgcagc cggaggtgaa cgcgctccgc gagaagcacg
      781 ccagcgaccc cgccaagatg aacacggcga tgatgaacct gtaccgggag cacaaagtga
      841 acccgatggg ggggtgcctg ccgatgctcc tccagctccc ggtgttcctc gcgctctacc
      901 aggtgctcct gcacgcgatc gagctccggt cggcgggatt cggctggtgg atcaaggatc
      961 tctcggcgcc ggacgtggtc gcctacctgg gcggcttccc cctccacgtc ctcccgctga
     1021 tcatgaccgg gagcacgttc ctgctccagt cccagacgcc ggtcgatccg cggcagcagt
     1081 tcatgatgta cctgatgccg gtcatgatgc tgtacatcat gtataatttg ccctcgggcg
     1141 tgatcatcta ctggacggtc aacaacctcg tttctgcgct ccagcagtac ctggtcaacg
     1201 tggccgaaga tcgccgcatg gcggcgcata cctgatcccg cgcgggccgc gggacggatc
     1261 ggacggaccg atgaacgaca gcgatgacat tcgagtcgaa ggcgagacgt ttcaggacgc
     1321 cgtggccgcg gccgcggccc agctggggct gaagaccacg gagctgggca tcgagatcat
     1381 cgatcccggc gcgtcggcgg actccagcac ggggtatcgc ccggtgcgca tccgcgcctg
     1441 gcgccgcgat cccgagtcct cggccgacgg gctgcgcaag cccgctccgg gcgcgcgccg
     1501 cgcgccatcc gattacgacg acggcggacg ccggagccac cacggcccgc cgcgccgccc
     1561 cgctccggtc aactacggcc cgccgccgcc gcccatggat cctgagaaga tcacgcccga
     1621 gctggtcacc atggtgcgcg agctcgccga cgggatcatg aagcacatgg agtttccctc
     1681 gacggtcgaa gcggagaaga ccaagcacgg cattcgcgtc gccattcagg ctggggactg
     1741 ggaccagtac ctgatcggcc ccgacggcga gacgctcgcc gcgctgcagc acatcatcgg
     1801 ccgcatgatc cgcgcgaaga tgccggacga ggcgccgccc cggctggagg tggacgtcgc
     1861 cggctatcgc gaccgccaga tcgaggcgct tcgcgaaatg gcgcggggac tgatcgccga
     1921 ggcgaaggat tcgggcgagg aagttacgac cgacccgctc cccgcgagcg agcggcggat
     1981 cgtccacctc gaagtggccg aggtcgcggg catggaaacg gtcacgatcg gagacggctt
     2041 ctacaagcgc gtcgtgatcc ggcgcgcctc cgcggacgcc gccgccaagg gcgaccggtg
     2101 acgaccggtg gcagacgacc cgggcgacac cattgtcgcg ccggcgacgg cccctggcga
     2161 agcggccgtc gccctgatcc gcgtgagcgg gccggccgcc ctggggctgg ccgcccgcca
     2221 cttccgcggt agccgctcgc cggaagagtc tccctcccac cacatcctct tcgggcaatt
     2281 cgtggacggc cggggcgatc cggttgacac ggtgctcgtc tcgatcttcc gcgcgccgcg
     2341 ctcctacacg ggcgaggacg tcgtcgagat ctcttcccac ggcggcgccg tcgttcccgc
     2401 cgctatcctc gacacgttga tccgctccgg cgcgcgtccg gcgcggcccg gcgagttcac
     2461 cgagcgcgcg ttccgaaatg gaaagctcga tctggcccag gcggaagcgg tcgcggcgct
     2521 cgtgcgctcg cgttccgatt cggcggcccg cgccgccggc gccacgctgg ggggcgcgct
     2581 ctcgcggcgc gtggaccgtt tggatgccgc gctggtcgcg ctgctcgccg aggtggaggc
     2641 gcgcatcgat tttcccgccg acgcggggga gccgctggac gcggtggcgc tggcccggcg
     2701 ctgcggcgcg ctcgcgtcgg agatcgagga gtgggtgcag gggcttcccg gcgcgcgccg
     2761 gcgcgaatcg ggcctctcgg tcgtcttggc ggggccgccc aacgtgggca agtcgagcct
     2821 cttgaacgcg ttggtgggct acgaccgcgc gatcgtgagc gagatcccgg gaacgacgcg
     2881 cgacacggtg gaaagctcca tctggatcga aggcatcgag ctgcggctga ccgacaccgc
     2941 cggactgcgc gagagccacg atccggtcga gcggctcggc atcgagcgca ccgcgtcggc
     3001 gctcgagcgg accgacctcg cggtcgtggt gctggaccgc tccgatccgg aagcgagcgt
     3061 gaccgcgctg ggttcgagcg ggtcgccgtc gcggccggtc gtcgtcgcgt ggaacaaggc
     3121 ggatctggct cgcggccggg ccgccatggg cggccgctgg gcgggaacgc cgctcgaagg
     3181 gcgcacggtc ctcgccgaag tcgagacggt cgcgatcgcg ccggccggtg ccgcgccgct
     3241 tcgaaacgcg cttcgcgact cattgccctc gattctcggc ggcgcggcgg gcgacgaggt
     3301 acccgcgacg agcgcgcggc aggaagccct gctcgccgat gcgctcgcgg cactccgccg
     3361 cgcggaagcg ggcctggccg cggacgaatc gtacgacctg atcgcggtgg atctgaccga
     3421 agcgcgccgg agcctgggcg agatggtcgg ccgcggcgtg gaccagggcg tgatcgcggc
     3481 ggtgttcgcg cgattctgca tcggcaaatg agcggcgggg ccgccgtcga gcattgggaa
     3541 gggagcggcg ccggcggaaa cggcgccggc gcccgaacgc gattcccgat cctcgtcatc
     3601 ggcgcgggtc acgccggttg cgaggcggcc tgcatcgccg cgaagatggg cgtcgaggtg
     3661 ggactcgtca cgatgagcct cgattcgatc gcccacatgc cgtgcaatcc cgccatcggc
     3721 ggactcgcca aaggccacct ggttcgcgag atcgatgcgc tcggcggaat catgggacgc
     3781 atggccgacc gcgcgggcat ccagttcaag atgctgaacc gcggccgcgg cccggcggtc
     3841 tggtctccgc gcgctcagga agacaaggcg ctctatcgcg cgatcgtccg ggagcatctg
     3901 gggcgcacgc cggcgatcac gctcgtccaa gcgcaggtgg tcgggctgct ggtggcggac
     3961 gggatggtgc gtggcgcgct taccgcggac ggtcgaacgc tggaagccca agccattgtg
     4021 gtgactccag gcacattcct gaatggattg ctgcacatcg gcgatcaaag catgccgggc
     4081 ggacgggtgg gcgaagcgcc cgcgcgaggg atctccgatt gcctcgtgga cctcggcttc
     4141 tccctggtcc ggctgaagac gggaacgccg ccgcgaattc atcgcgactc gatcgacttc
     4201 tcgcgctgcg tcgagcagcc gggcgacgat ccgccgcgcc ccttctcgca cttcaccggc
     4261 gcgctccagg tcgaccaggt gctctgccac ctcaccgaga cgaacgcggc gacgcacgat
     4321 ctgatccggg cgaacctgca ccgctcgccg ctctacacgg ggaagatccg cggcatcggc
     4381 ccgcgctact gtccgtcggt ggaagacaag gtcgttcggt ttgcggagaa gcccgcgcac
     4441 cagatcttcc tggagcctga gggacggagc acgcacgagt attatgtgaa cggcctctcc
     4501 accagcctgc cggaagacgt gcagcgggcg atgttgaaga cgatcccggg gctggaagat
     4561 gcggagctac tgcgcacggg ctacgccgtc gagtacgact tcgtcccgcc cacccagctt
     4621 cgcgcgacgc tcgaaacccg tcctgtacgc gggctctacc tcgcgggaca gatcaacggc
     4681 acgtcgggct acgaagaagc cgcggcccag ggattgatgg ccggcatcaa cgccgcctcg
     4741 cgactcgtgg gcagggaccc gttcgtgctg cggcgcgacg aggcctacat cggcgtcctg
     4801 atcgacgacc tcgtgacgaa ggggaccgag gagccgtatc ggatgttcac ctcgagcgcc
     4861 gaacaccggc tgctccttcg ccaggacaac gcgccggagc ggctcgcgca tcacgcgcgc
     4921 gctctcggga cgctcgcgag cgatgagctc gacgcgctcg acgctcgagt ggtggcacgg
     4981 cgcgacgccg tctcgaggct gcgtgccgtg cgcggctccg gctccatcac caatcgacca
     5041 cacgagacgt tgcccgtgat ggacgagatc caggaatcgg cgctgatcga gatccgctac
     5101 gagggctaca tcgagcggca gcgccgtacg gtggaacgcg cgtccaggaa cgagagtctc
     5161 ggtattcccg aggacatgat cggacaggaa ctgaagggac tctcgcgcga agcccatgag
     5221 aagatcgccc gcttcaagcc ctccaccgtg ggccaggcct cacggctccc gggcatctct
     5281 ccctcggacg tcgccgttct gacgatctat gccgaacgag agcgccggcg cggaccccgc
     5341 gccactcccc gctgagctga ttccggactt cgagcgcttc cggtcagagc tgtccgctgt
     5401 ttcccgggag gcggcttggg ccggcctctt tggggatcct cgagccgaag ccggatggcg
     5461 cgagtacggc cgccttcttc atgagcggtc ggcggccctg gctttgatcg ccaaaggcga
     5521 ccgcggcgaa gtgttcacga gacacatcct ggactccctg aatcccgtcg cgctgttcga
     5581 cacgccgcca gccagcctgc tcgacgtggg gagcggaggc ggattgccgg ggattccgct
     5641 cgccatcgcc tggcccgacg ctcgcgcgct gctgctggag tcgcgcgagc ggaaggcagg
     5701 tttcctggag ctcgcgtccc gccagctggg gctgcgccac gtgcgtgtcg cgtgcacccg
     5761 gctcgaggac ctcgagagcc aatggcgcga ggagcgggtg gaggccgtgt tcgtccgcgc
     5821 gctgggtgac ctggatcgtg tgctgggcct ggccgcggcc gtcgcccgcc ccggcgcgcg
     5881 gtgggtctac tttttgggca tgagagccag cggggaggcg cttccctctg ggagcccgtt
     5941 cgacccgcgg atcgtcgagg gtgtgttcgg gggaaggctg ctgacgggag tcttcccggg
     6001 ctaggtttct ccgcaggccg gatccctcgc agcctaggtc tccccgcagg ctaggtctcc
     6061 ccgcagccta ggtctccccg cagcctaggt ctccccgcag gctgctccgc cctgtctgtc
     6121 tcccgctgac tttgtggaag gcccttcagg acgagcacct tttcgatggt caaccgccaa
     6181 ccggtcggcc gcttgtgcgc atgtttcacg tgaaacatgg ccgcaatcta ctgcaaacat
     6241 gtccgttgtc ccgggggcgc cgaaactaac cggccagttt cacggtgcct gggctcgccc
     6301 gcgggtcgag tgaacatgct ttaaagcatg tcgccgggga tcaagccaac aagctttgaa
     6361 gcatgcccta tatgactcac ctggggtttg cccgggccgg cgctacctgg tctctgtctc
     6421 agcgatcaaa cgaacatgcc ccggggcatg ttgtcgtagg ggcgtcccgc tgccacggac
     6481 agccgaacgt gcttcagggc aggctaggca tgtactcggt caacatgctt tgaagcatgt
     6541 tcgctgggtt tgggaatcga tcctcgcccg ctccggagtg gcgcgctcgc cggctctcca
     6601 aacatgcttt gaagcatgtt cgctggactc gccatgaggc gtgccctggc gggtccatca
     6661 tgctccgcgg ttgaacgaac atgccctggg gcatgctgtc ctgggcctcc gggtgaacat
     6721 gctttaagca tgttggtctg atcctcgcgg gacgcccatc tggagtcaac tcgccccggg
     6781 gtgagttcaa atctcattcg acggcgggcc ccgagcaccc ggcgccccaa cacgcccccc
     6841 acccgtgaaa cacccactta gtttcgggct cgccggggca acgcacaatt tcacagccag
     6901 atatggccat gtttcacgtg aaacatggaa tcgctggcgc tccgccgggt tctcccctac
     6961 actgcgatct ccccgatttg gacaacaaaa cttcggttcg tggacagggt ggtggtgcga
     7021 tgccaaggac ggtagcggtg gcgaaccaga agggtggcgt cgggaagacg acgaccgccg
     7081 tgaatctggc ggcggcgctc gcgatggcgc agcgtcgggt tctcctcatc gatctcgatc
     7141 cccaaggaaa cgcaggcagc ggtctcggct atcccggcgt gaaggtgacg cgcgggatct
     7201 atgacgtcct gacctcacac gccacgctgc gcgaggtcct gatggaaacg gaagtgcccg
     7261 gcctcagcct ggtgccttcg ggccagcggc tcgtgggggc ggaggtggag ctggtcggcg
     7321 tggaccgccg ggagtaccgg ctcaaggacg cgctgcgcga gatcgaggga gatttcgatt
     7381 tcgtactcat cgacgcgccg ccctctctcg gcttcctcac gatcaacgcg ctgacagccg
     7441 cacgctcggt gctggtgccg gtccagtgcg agtactacgc cctggaagga ttgagccacc
     7501 tgctcggggc gatccgcctc gtccagcagg ggctgaaccc cgagcttcag atcgagggcg
     7561 tactgctcac catgtacgac aaccgcctga atctgtcgca gcaggtgctg gaggagaccc
     7621 ggcgtttctt cgcggagcgc gtctaccgaa ccgtgatccc caggaacgtc cgcctcagtg
     7681 aggcgccgag cttcgggaag cccgtcgtca tctatgatcc gcagtgtgtc ggagccacga
     7741 gttacgtgaa cttggcgagg gaggtgttgg aacgtggtta gaaaagcgct tggtcgaggg
     7801 ctcgaggctc tgattcccgc agcggtcgag cgcgaggtgc cgatcatccc ggagtcgcgg
     7861 ccgccggaag ccagggccgc cgaggcacgg cccggggacg ttcagcacat cccgctcgcg
     7921 aagatcgttc gaaatccctc gcagccgcga acgcagttcg atccgtctgc gatccaggag
     7981 ctcgccgcct ccattcgcga gcgcggggtc ctccagccgg tgctcctccg ccccgcgggc
     8041 gacggctacc aattggtcgc cggcgagcgc cgcttcctcg cggcgcgcga ggcgggcttc
     8101 gcctccatac ccgcgatcat ccgtcccctc accgatcgcg agtcgctgct catcgcgctg
     8161 atcgaaaaca tccaacgcga gaatctcaac cccatcgatg aagcgcgcgc atactaccga
     8221 ctcgcaacgg aatacggcct tcaacacgaa gagattggac agcgcgtcgg caaggatcgt
     8281 tccaccgttt ccaacgcgat tcgattcaac aacttgcctg ccgatgtcca gtccctcatg
     8341 gagtcgggcg cgctcagtgc cggccacgga cgcgcgcttc tctccctctc tacgtcgaag
     8401 cagcagagcg acctcggagc gtctgccgcg tcgcacgcct ggtccgtgcg cgatctcgag
     8461 gagcggatcc gccgctcggg cgcccctcgc gccgccaagg cgcgccgctc tccccgccgg
     8521 gctttttccg ccgagctcat ggccctcgag gacgagctca tgcgggtgat gggcgccaag
     8581 gtcaggctca cgcggcgccg cggcggctcg ggcagcatcc tgatcgagta ccataatgag
     8641 gaagagctcg agcggatcct cggcatgctc cgggacatca gcccggctgg ccacgcctga
     8701 gcgggccacg cctgagaagg atgcaacctg gccccgcctg cgcgtgtcct accgtggacc
     8761 gggacggcac atgagaaacg ccgctccgaa agcgcccgct cccgggtttg tggataactc
     8821 gccaaaaccg gtggaaaacg tgataatcgg cgccggcgca agggctttgc aaacagtaga
     8881 cgatgagaac ggggcaggtt atgcacagag gcccaaagta ggcatacggg cgcggctttt
     8941 cccgcgaccg ggccatttcg acgcacactg aggaacccta catgacgcat tcgaccgcat
     9001 ccggcgacgt ccggctgctc gaggagctga gtaccgcccg gggcgagatc ctgaccgagc
     9061 tcaggaaagt gatcgtcggg caggacgtgg ttgtggagca gctcctgacc gtgctcttcg
     9121 ccaacgggca ctgcctcatc gtgggggttc ccggcctggc gaagaccctg ctcgtctcca
     9181 cgctggccac catcctggat ctgcagttca gccgcatcca gttcacgccc gatctcatgc
     9241 cctcggacat cacgggcacc gaaatcctcg aggaggaggc gggcaccggg caccgttcct
     9301 tcaagttcat ccatgggccg atcttcgcga acgtggtcct ggccgacgag atcaaccgca
     9361 cgccgcccaa gacgcaggcc gcgcttctcc aggcgatgca ggagtaccgg gtcacggcgg
     9421 gcggcacgac ctacccgctc tcgctcccgt tcttcgtgct ggcgacgcag aacccgatcg
     9481 agttcgaggg cacctatccg cttcccgaag cgcagctcga ccgcttcatg ttccacatct
     9541 cggtcggata cccgagcgac gtcgaagagg agcggatcgt caccaacacc acgagcgcct
     9601 acgaagcgca tcccaagaag gtgctctcgg tggagcgcat catcgacctg cagcgcctgg
     9661 tgcggcgcat tccggtctcc gagcacgtcg tgcgctacgc cgtccggctg gcgcgcgcat
     9721 cgaggcccgg cgagaacgcc gcgcccttcg tcaaggactg ggtctcgtgg ggcgcgggcc
     9781 ctcgcgcggc gcagtatctg gtgctgggcg ccaaggcgcg cgccgcgctc gcgggacgtc
     9841 cgacgcccag cgccgacgac gtgaaggccg tagcacccgc ggtgctccgc caccgcatca
     9901 ttcccaactt caccgccgag gcggacggca tctccacgct cgacatcatc gccaagctcc
     9961 tcgaagccac gccgagcgtc gacgggaagc acgcgtaggc ggggcggcgc cccgcccgtg
    10021 tgccgttcca tgcgtgagcc cagcccttcg ccgcacgagg tgcatccttg agcccggagc
    10081 gcgcccggga gcgcacccgc ctgctcgatc cgcatttcgt gtcgaagctc gctcgcctcg
    10141 acctgaaggc gcggctcgtc gtggagggct tcctgacggg gcttcaccga agcccgtacc
    10201 acggcttcag cgtggaattc gccgagcacc ggcagtacat gccgggcgat cctctccgtc
    10261 atctcgactg gaaggtgctc gcgaagtcgg atcgcaagta cctgaagcaa tacgaagagg
    10321 aaaccaatct gcgcgcgtcc ctgctcgtgg acacgagcgc gagcatgggc tacggatcgc
    10381 acgggatcac caagctcgac tacgcgcgcc agctggccgc cgcgctggcc tacctgatgc
    10441 tcacgcagaa cgacgcggtg gggatgttcg cgttcgcggc cgggcgcggc gagcacatcc
    10501 ccgcccgctc cacgatgggg catctccgtc cgctcctggt cctcctggac cgtctccagc
    10561 cggggggcgc gaccgacttc gccgcttcgc tccattctct cgcggagcgc ctgacgcgcc
    10621 gcggcctcgt cgtgatcctc tccgatcttc tggacgaacc ggagcggatc gcgcaggcga
    10681 tccaccattt ccgccaccgg aagcacgagg tgctcgtctt ccacatcctc gacccgatgg
    10741 aggtctcgtt cgatttccag cgcgaggccg tctacgtgga tctggagagc ggcgagcggg
    10801 tcacgacccg gccgcgcgag ctgcgctccg actatcgcca gcgcgtgagc gaatggcgcg
    10861 agacgctgcg gcaggcatgc atcgagaagc gcgccgacta cgttcccctc acgacggacc
    10921 agccctacga tcgcgcgctc ctcgagtatc tgtcgaagcg cgcccgtctc ctgtgagcgg
    10981 ccggcgctga tgccgaccct ctcgtttctg aatcccgcgt tcctctgggc gctgcccgcg
    11041 gcgtcgctcc cgatcctgat ccacctgctg agccgccagc gcctgccgga agtgcgcttc
    11101 cccacgacgc agttcctgcg ccagctggag ccgcgcgaga ttcgccgcct ccggctgcgc
    11161 gagatcctgc tcctcatcct gcgcacgctg gcgctcctcc tctccgtgct cgcgttcgcg
    11221 cggccgtcgc tggcgccgcg cggcgccgtg acgcacgcgg ccgcggccgt ggggatcctc
    11281 ctcgacgatt cggagagcat ggccgcgctg gacgaccgcg cccgtccccg catggagggg
    11341 gccgtggcgc gcgcgctcgc gatcgccgat gccggccgcg ccggcgacga gctctacctg
    11401 gcgcgcgcga cgcggcctgg ggaggcggag atcggccgcg cgcgcgaccg cgtgcgcctg
    11461 cggcgctcca tcgagcggat cgaggccgag ccgctgccgg cgcgcctgga aagctcgctg
    11521 cattccctgc gcctggctct cgggcgaagc cctctcaagg cccgagagct ctacgtgatc
    11581 agcgatcttc agcgggccag tctcacgccg gaagcgcggc gcgagatcac cgaggcggcc
    11641 gcggcgggaa tccgggtcat tctcctcccc gtgtccgagg gccgcacgcc caaccacgcg
    11701 ttcaccgccg tcgatccggt cacgcggccc gggcccgagg ggcgcgggct cgaggtccgg
    11761 gcgcggctcg tgaattacgc cgacggcccc tccgatcgcc tcgcgctccg ggttcgccgc
    11821 ggcgaggcgc tggtcggagg cggcgacgcc gcgctcaagg gcggggaatc gcgatgggtg
    11881 tcgatgccgc tcgagcccgg cgcggtgaac gatccggcga ttcccgtcgt cgccgagtcg
    11941 gacgaagacg cgctgccgct cgacgatcgc tggtatgccg tgctcggggc gccccggcgg
    12001 ctccgcgtgc tccggatcgc cgagccgcgc ggtgccgcac ccgcgccgcg attcgccgcg
    12061 ctcgccctgg acccctcggg cgacggcacg tcgggcttca ccgtcgagac cgccgggccc
    12121 agcgctctcc tcggactctc caaatcgcgc gccgacgtcg tcgtgctcga ggacgtggcc
    12181 tcgctctcgg ccgacgccga ggcgaggctc aaaggctacg tgaaggaagg gggcggactg
    12241 gtcgtggccc tgggcccgca cgccgatccc gagtactaca cgaagcggct cttccccggg
    12301 ctcgtggacc tgaccctggc gggacccgac cgctcggcgg aggggacctc gttcgagctg
    12361 cgcgcgcgcc ttccgggcca cccgatcctc gagggactcg ccctcggcgt gggttcgccg
    12421 ctcacgcagg cgcggctctc ggggatgatg cgaagccgca cggcgtcggc gcgcgccgag
    12481 gtggtcgtgc agaccacggg cggccttccg ctcgtcgtgg cggcgccctc ggtcgcggtc
    12541 ttcctctcct ccttcgccga cgactggggc gacctgccgt attccggcgc gttcgttccc
    12601 ctggtgcgcg ggctggtttc ctacgccgcg cgcgcctcgg agagcgagcc cggctcggag
    12661 ccgcgcgtgg gggagcgccc gttcgcgcgg ctcgaggcag ccccttcggg agcggtcgtc
    12721 gcgcgcgggc ccgcgggctt cacctcgccg gcagccgtgg agaacgaggg ggcggcgttc
    12781 cgcgccgtcg ccgacgcccc ggccatggcc gcggggttct acacgttcga ggcgggtggc
    12841 aaggcgctcg cgaccgtcgc tctgaacccc gacccggtgg agagcgatct ggccgcggtg
    12901 ccggccgact cgctgaaagc cgacgcccgg gggccgaacg gagcgcccgt gctctccatc
    12961 gacaaccgct cggcgctcgc gacccggctg ggcgatacgc ggcgcgggcg cgagctctgg
    13021 ctgccgctcc tggttctcgc cggggtcttt ctcatcggcg agatccttct cggcagcgcc
    13081 cgcgtcctcg accgttagag ccatgaacgc cgtccgcgag ccctcccgag aaacgaatcc
    13141 cggcgatccg tcaccggtgg cggcccgcct gctcgaggcg ggcggcacgc acccgcgcat
    13201 ccacgagctg gagacgcgcc tggacgcggc cgcgcccggc gcctcgatcc ggcttcgcgg
    13261 cgtcgccggg agcgccggcg cgctggtcgc ggcccttctc gcgcggcgcc cgggcacgcg
    13321 cctcgcggtg atcgcccccg acctcgaccg tgccgaggga tggcacgacg acctctgctt
    13381 cttcctgggc cccgaccgcg tggtcttcat tccgccgcac gacaccgtct cctggagcgc
    13441 gcaggtcgca accgccccgg tgcgcgagga ccggatccac gccttcctgc gcctgggcga
    13501 tcccgatccg cccgtcgcgg tgattccggc gctcgcgctc cacaggcttg cgcccgcgcc
    13561 cgaccgcatc cgcgcccgcg cgatcacggt gcgcgcgggg ctttcgatcg ctcccgaggc
    13621 gctgctccgt cgactggtgc tcgccggcta tcgctccgtt ccggagatcg gcgagtcggg
    13681 ggaggtgagc cgccggggcg gcatcgtgga cgtgttcggt ccggggctgg accatcccgt
    13741 gcgtctcgag ttcgacggcg acacggtggc ctcgctccgc tccttcgacg tcacgtcgca
    13801 gcgctcgacc ggcccgatcg aggaggcgcg gttggcgccc gcgcgcgagc tgctctacga
    13861 cgaggacgtg gacgcgcggc tccgccccct cgacgcgccc gaggcgccgc gcacggccga
    13921 gaccgaccgg atccgcgacc tcgtggccga gggaatctac ttcgagggca tggaatgggc
    13981 ggcgcccctc ctcggccttc cgcttggctc ggtgctcgac cacctcgccc cgaacaccat
    14041 cctctgggtg gacgagccgg cggcggtgga gcgcgagctg gaagatgccg agcgcgagac
    14101 cgagcggctg gaggaggacg cccgcgtgaa gacgccgcac ctgccgccgc gggaagcgct
    14161 cttcgattct cccgaacgcg cggccgggcg gctggccacg cgccgccggg tggaagcggc
    14221 gctcaccacg cgcgagggag cctcgcgcgg cgaggcgatc gccctggacg cgcgcccgca
    14281 gccctcgttc gggcgaaagc tcgatctcct gcgcaccgag ctgaagcgcc tgagcgacct
    14341 gggcatgacc cgcatgatcc tctgcgacaa ccggggccag gccgagcggc tcgaggagct
    14401 cctcggcggg gacctgggcg gcatcgacat cggcgacctg agcgccggct tcgtgctccc
    14461 gggcgcgaag ctcgccgtct tcaccgacca cgagatcttc gcgcgctatc ggcggcgccg
    14521 cgcgcggaag ctccgcccct cggcggccgc gctccgcgag ctcctgaccc tcacgcccgg
    14581 cgactacgtg gtccacctcg atcacggcat cggcgtcttc cgcggcctca agccgctcac
    14641 gctggatggc caggagaccg agtgcatgca gatcgactac gcgggaggcg accgcctctt
    14701 cgtgccgatc gggcagctcg ggatggtgga gcgctattcc gccgaggagg gacgcgcccc
    14761 cgcggtccat cgcctcggag gcaccacgtg ggcgcgcgtc aaggcgaaga cgcggaaagc
    14821 gatcgccgac atggccgaga cgctgctccg ccactacgcg gtgcggaagg ctcgcccggg
    14881 gcgcgccttc ggtcccgata cgccgtggca gcgggagctg gaatcttcct tcatctatga
    14941 agagactccc gatcagctcc gcgccatcga agaggtgaag aaggacatgg aggcgccgcg
    15001 ccccatggac cgcctcatct gcggcgacgt gggctacggc aagaccgagg tggcgatccg
    15061 cgccgccttc aaagcgatcc aggacggcac gcaggtggcc gtgctcgtgc ccaccaccgt
    15121 gctcgcgcag cagcacttca ccacgttcag cgagcgctac gccgactatc ccgtccgcgt
    15181 cgacatgttg agccgcttcc gctcgcccaa ggagcagaag gagacggtcg cgaagatcgc
    15241 aagcggcgag gtggatctcg tgatcggcac ccaccgcctc ctctccaagg acgtcgtctt
    15301 caagaacctg ggtctcgtgg tgatcgacga ggagcagcgg ttcggcgtcg cgcagaagga
    15361 gcggatccgc accctcgtcg agacggtgga cgtgctcacc ctcaccgcca cgccgatccc
    15421 gcgcacgctg cacatgagcc ttctgggcgc gcgggacatg tcggtcatga ccacgccgcc
    15481 gcgcggacgc tatccgatcc ggaccgagat cgtggagatg agccaggacg tggtgcgcga
    15541 ggccctgctc cgcgaatccg accgcggcgg gcagtccttc ttcgtgcaca accgcgtgga
    15601 atcgatcgac cggatcgcgc actacgtgac cagcgtcgtt ccccaggtca aggtggtggt
    15661 cgcgcacggg cagatgaagg acacgcagct ggagaaggtg atggtggagt tcctggagcg
    15721 gcgcgccgac gtgctcgtct ccacgatgat catcgaatcg gggctcgaca tccccaccgt
    15781 gaacacgatg ctggtgcatc gcgccgacgc gctgggcctg gcccagctct accagctgcg
    15841 cgggcggatc ggccgctcgc accaccaggc gttctgccat ctgctcgtcc ccgcggggaa
    15901 agtgctgacc gaagaggcgg agaagcggct ccgcgtgatc gccgagcacg aagagctggg
    15961 tgccggcctc atgatcgcga tgcgcgacct cgagatccgc ggtgccggga acctcctcgg
    16021 tcccgagcag cacgggcaca tgctcagcgt cggattcgac ctctactgcc gcatggtgga
    16081 cgaagtcgtg cacgagctgc agggcgaacc ggtggaccgc cgccccgagc ccgaggtctc
    16141 gagcccgctt cccgccttcg tgcccgacga gtacgtcgag gatcgggacg agaagctgga
    16201 cgtctaccgc cgcatggccg cgctctccac gctcgaggcc ctggaggcgc tgcgcgccga
    16261 gcttcgggac cgcttcgggc cggcgcccgt ggaggtggag cacctgctgg ccctgaaggg
    16321 gcttcggatc ctgggcaagg aggccggggc ggaccgcctc cggatcggcc gcgaccgcat
    16381 cgaaatcgag ctgcgcgagc cgcttccccg cgagcggatc atggcgctcg tgcccgaggt
    16441 gccggtcccc atcgagttcg cctccggggc caggacgctg aggatccgga agcccggcga
    16501 ccccgtgccc ttggcgacaa aggtgttgcg ggccctggca cccgttgata gcgttgcgcg
    16561 cctgcctctt ccggcggccg gaaggtgaac tccggggcgc cctccggggt acagagggag
    16621 agcagcgcca ccgaggagac tcgccgccac acgtccctat gattcctgag accgaaaaat
    16681 ccacgccccg gcggtcgctt gggcccagcc ggacggctgc cgcgcgtgcc gcggccgccc
    16741 tgttggccct gatcgcgctg gcggtcccgg cgcccggacc ggcccgcgcg gctgcgccgc
    16801 cgtccgcgcg cggcccgatc gcgacggtgg gagaccggac cgtggacgcc ctggacatcc
    16861 agagcgcggc gctggtcctg ggacaggacc ccctgcgcaa gacgaacccc gctctctggc
    16921 ggcgcatgct gctcgaccgc tgcgtcgacc gcgagctgct ggccatggag gccgagaagc
    16981 gcgggatcgg gcgcgacccg gcgatccggc agcggatcgc ggagcgggaa tacgcggcgc
    17041 tcttccaggc ggtctaccag cgcgtcctcg tgccgacgct cgacgtcacc cccgcccaga
    17101 tggacagcct ccagaagacc ggcctctacc ggatggtgga cctctactac atcctcctcc
    17161 gcgacgacgc cgcccaaggg aggcgcaagg aagcggagac gatcgtggag cgcctccggg
    17221 ccggcggacg gttcgattcg gtcgcggtca cgaagagcgg acacccctcg cgcggaaacg
    17281 gcgggcattt cggcgccgtg ctcgcgcgcg acctcgaccc ctcgtcctac gcggcgtccc
    17341 gcacggcgaa gccgggagac atcctcggcc cctattcggg cccctacgga cacgagatct
    17401 ataaggtagg gggcttccag acgctctccc ccgactcgat ccgcgcgatc gtgcgcgtcg
    17461 agcgcacgcg gaacgtgatc cgcgactatc aggcgcggct tctggcccaa taccacttcc
    17521 tgctcgagcc cgaggtggtg cggccgatcc tcttcgcgat ggcgagcgag acgcccgatt
    17581 cgctcctggc ctccatgagg ccggacggga cgcggccgcg gatgggcgcc cgccccgaga
    17641 tcggcgtgat cgcgcgggtg gacggggatt cgctgacgct ccccgacctg ctgcgcgaaa
    17701 cgcatcccgc gcccggcgcg aacggccgga tccggatccg cgacggcgat gcgctgcgcg
    17761 acctggcctc cgaggcgctc ttccggcgcc ttctggtgcg cgacgccaag gagcgcggcc
    17821 tggccagcga tccgcgcgtg gcgcgcgagc tgcgcctgat ccgcgagggc accgccgtga
    17881 ccgccatgat cgcggccgag cgccccgccg atccgggaac gccggagctt cgggcctggt
    17941 tcgaggcgca cgccgcgcgc taccggcgtc ccgcggcgcg acgcgcccgt gtggccgtgt
    18001 tcgccagcca ggacagcgcc atggcggcgc tccgctcctg gaacggggtc gggatctccg
    18061 actcgtcgct ccaggcgctt cggttcgagg agcagccccg cgcgacggag tcgacgctct
    18121 acccgcagca cgcggcgacg ctgccctttt tcgacgccga ttccgatccg ctcggcatcg
    18181 ccgtacggca tctcgatccc ggcatgacgt cgccggtggt gcggaccgcg caggggtacg
    18241 cgatcgcgca cgttctcgcg cgcgaacccg cgcgccccat gaccctggac gaagcgatcg
    18301 accgcgtgcg ccgcgattgg cgcgaggaga aggaaaacga atgggtgctg agtcagttgg
    18361 agcgcatgcg ggcgcaaacg ccggtgcggg tcgtgccgtc ccggctgcag gcggtgaagc
    18421 tctcgccggc cccggcagcc acgggaagct tgggagcggt ggccggaaac gaggggggcc
    18481 gatgatccgg cgctccctga tccccgcgct cgcgttcgcc gcgttggccg cggcggcgct
    18541 cgccggcgga ccgacgggag ccgccgagcg gatcgccgcg atcgtgaaca agtcggtcat
    18601 cctctcctcg gacgtcgacg accagacgca gcaggccgcg gcgcgcttca acgtcgatcc
    18661 ctccgacagc gcgtcgatga agaagctgcg gaaagaggtc ctggaccagc tggtcgagaa
    18721 gcaggtgatc ctggccgagg ccacgcgcct gggggtcgtc gtgccgccga aggacgtgaa
    18781 cgaggcggtg aaccaggaga tcgagcagct caaggagcgc ctcggcggcg ctcaggcctt
    18841 ccaggcggcg ctcacgcagg aaaagacgac cgaggccgcg ctccggaagc gctacgagcc
    18901 ggacgtgaag gaccagctcg tcatcatgcg caccgtgggc cgcgaagtgc agaacaaggt
    18961 cacggtgacc gacgccgagg tgcgcgccta ctaccaggcg aaccgcgact ccatcgcgaa
    19021 gaagccggag tcgctcaagc tggcccacat cctcgtggcg ttcgagcccg attcccagca
    19081 ggtgcgccgc gcgcgcgtgc gcgccgacag catccgcaac gtcctcgcca agcccgggaa
    19141 agcggccacg ttcgccgaca tggcggcgca gctctccgac gacccgagcg gccggcgcgg
    19201 gggcgatctg ggcaccttcg gcaaagggga catggtcccc gagttcgagg aggtcgcgtt
    19261 cgccttgaag ccgatggaga tctccaatcc ggtccggacc cgcttcggct atcacgtgat
    19321 ccaggtgctc gagcacttcc ccgccaccga cagcaccgag gagcgggtgc acgcgcgcca
    19381 catcatgatc gcgaccaagc cgtcgccggc cgacgaggag cgcgcccgca cgcgcgcgct
    19441 cgcgcttcgc gactccctca cgcacggcgc cgatttcggg acgctcgcgc gcaagtattc
    19501 ggccgacgtg gccaccaagg actcctcggg ctatctgggc gagatccccg tgcccagcct
    19561 gccccccaac atgcgcgagg tgctgacggc gctcagggaa ggggagatct ccacgccctt
    19621 caagcgcgac gccggctacc acatcttcaa agtgctgggc cgcagtcccg agacggagta
    19681 caagtacgag gacatcaagg acgacctgaa gaaggtcgtg acgaaccgga agctcgaaga
    19741 ggcgtaccgg c
//
LOCUS       DASSLZ010000214         9247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1335907, whole genome shotgun sequence.
ACCESSION   DASSLZ010000214 DASSLZ010000000
VERSION     DASSLZ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335907"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFM00_08260"
     CDS             complement(<1..500)
                     /locus_tag="VFM00_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498279.1"
                     /translation="MTAEAKGRRAAAQETHPPFKPAPHEESSAAPTPRVTSLHETAPP
                     ASHPAPAASAPPFSIAAFIAALVAGLVGHFLPGAMGFAAAVVAAIFGAVVVALLSGSV
                     ETRRAASEELVHERERASLAADQLEKSRRHADLATVQFREGKDPLTEVPELRVGPLRE
                     LLEAAR"
     gene            complement(503..997)
                     /locus_tag="VFM00_08265"
     CDS             complement(503..997)
                     /locus_tag="VFM00_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7498280.1"
                     /translation="MRRESGTALGTRGLIAFPVGEILFGVPVEDVAGLIEADHIAPLP
                     RRSGAVSGVTAFRGTMVPVVDLCEWLGVSGPTGDAPRYGVVLSRGTERFALLIPSMPR
                     LWPGREIKESDESIADGELAAVIGSVYLAGAERIHCLRCWSIFDAVMPPVAAGSDSDS
                     IEER"
     gene            1528..2238
                     /locus_tag="VFM00_08270"
     CDS             1528..2238
                     /locus_tag="VFM00_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498281.1"
                     /translation="MNCLLLTKAEGTPQNGDPLPQEDVDGYLAALKDQLEAQVPLPPE
                     RSRLLSRYDVAAWRRLASAHRGSLRYLIYRTPEDLLLISVGSFEQTGPYVPNRREGLL
                     PGEEADVGRSPNWYHFSYTYGELLENPGQTLSAVLEKLTVSLERYNKMLSHMRGEHWS
                     LAKRFKKAQVTRLSRTVDEDARDESLRVMQDRLLDAYGEWKRTGERRHLDEIRGLARE
                     IRLLAPDFQFSLPSGGDA"
     gene            complement(2395..2850)
                     /locus_tag="VFM00_08275"
     CDS             complement(2395..2850)
                     /locus_tag="VFM00_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7498282.1"
                     /translation="MAPTIRPARPNESDAIANVFLHSWKTLMPDIPIGHTDDQVRAWI
                     AKSVMVQKRVFVATENDAVIAMMAISEDGEGGWIDHLYVAPESVGRGVGTALLEHALA
                     ILRAPVWLYAFQSNERAREFYEARGFSVAKFGDGTGNQVGLPDVLYRLD"
     gene            complement(2918..4372)
                     /gene="guaB"
                     /locus_tag="VFM00_08280"
     CDS             complement(2918..4372)
                     /gene="guaB"
                     /locus_tag="VFM00_08280"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7498283.1"
                     /translation="MPVDYALTFDDVLLLPGYSEVHPRDVDTSTLLTREITLNTPVVS
                     AAMDTVTESALAIAIAREGGLGIIHKNLPVLEQVEEVDKVKRSESGMIVNPITLPPDV
                     PIARALELMEKFRISGVPITEGKRLVGILTNRDLRFVKSPDLKVRDVMTKENLVTAPV
                     GTTLEEAERILHTHKIEKLPVVDDQYSLRGLITVKDIQKRIRYPRSCKDHLGRLRVGA
                     AIGVGQDANERVAELVRAGVDVVVIDSAHGHSKAVIETTRAVKNAHPNLQLIVGNVAT
                     ADGARDLIRAGADCVKVGMGPGSACTTRVVAGVGVPQISAVLECANEAAKAGIPIIAD
                     GGIKYSGDLVKALAAGAHSVMLGNLLAGTEESPGETLLLEGRTYKVYRGMGSLSAMSS
                     GRGDRYFQEGVRDLKKLVAEGIEARVPYKGPVANVIYQLIGGLRAGMGYCGVGTIEGL
                     RTQTRFVRITQSGLRESHPHDLTITKEAPNYEIR"
     gene            complement(4445..5125)
                     /locus_tag="VFM00_08285"
     CDS             complement(4445..5125)
                     /locus_tag="VFM00_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7498284.1"
                     /translation="MSRTVVGGLLALLVIALPLTATADDLTGFGGIGFRGGLSKFGSE
                     ANTPLSLTNNQPLSDGTKPRFSGDLIFSYVFGSHVWGDVTVGYAWNRLDTDDNRFWLE
                     SAVPITGGLRYLLHEGRTMRPYVGAGGGLYVWSIQSKDLGAAKDPSTFERFRRADPGF
                     YGMVGVERTMSKHISTTADAVYHHIFAQNTADFPSGYNGNKSYAQIRLGVTFYFTLTE
                     RIDTGLPE"
     gene            5336..5410
                     /locus_tag="VFM00_08290"
     tRNA            5336..5410
                     /locus_tag="VFM00_08290"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5368..5370,aa:Met,seq:cat)
     gene            5804..7918
                     /locus_tag="VFM00_08295"
     CDS             5804..7918
                     /locus_tag="VFM00_08295"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET7498285.1"
                     /translation="MRLHPCIKPVLRWSGVALTAAIILVLGASAGAQSVLDPLSLAKY
                     VDPLPIPAAIDATMPGSSFEIGAYQIQQKLHRDLPLTTVYGYGLTEATATYPGPTIVA
                     RHNVPIQIRWTNHLPMTHILDYAIDPTLMRAETSTGVPITTHVHGAEVEPQSDGGPNT
                     WFTKDFAETGPDFTSQVHTYVNTQLASTIWYHDHAFGYTRHNVYAGLAGYYLITDPGN
                     EPPGLPEAPYDMGLAIQDRMFTTDGQLWYPNEGVTSVHPKWIPEFFGNVILVNGKVWP
                     YLNVEPRKYRFRMLNGSQARFYSLALLERVGSTPGPAFYQIGTDGGYLAEPVLLNDPA
                     NVHSPRLVIAPGERADVVIDFSAYAPGTEFLLRNTAKAPYPMGDAPDGQTTGQIMLFR
                     VVPSTGPDNSVIPPALATVNRLTNPTVTRTMTLNERLLGDDPLGALLNGMPYDAPATE
                     YPVLGTTEMWEIANLTGDTHPIHLHLVQFQLLNRQKLNARRYQMAFDAANPTLPSQTY
                     VPVPVGPYLKGKPTPADANERGWKDTFRMNPGEVTRVLVRWAPQDESPSFAFDATADP
                     GYVWHCHILEHEENDMMRPFHVVAPGAATAGGASVNAAPAFAPASLPGGTVAFRASNP
                     ALEGSNLRFSLTRAGSVALDLFSVAGRHVATLASGWYEAGDHDVSWTARATSGEPLAN
                     GVYVVRFRGDGVERAQKMVLVR"
     gene            7983..>9247
                     /locus_tag="VFM00_08300"
     CDS             7983..>9247
                     /locus_tag="VFM00_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HET7498286.1"
                     /translation="MNPRFARTSALFLSAAAVLVAAPASAQFTALTKAPLGDRGSAFG
                     VAWADYDLDGDLDLYLTEDGPNLLLRNDGGDNFTDVTSPPLDEGGNGSAAVWGDYDGD
                     GDPDLYLVNFNSPNHLYRNDRAAGFTDVTAGPLGTNGPGQSAAWADVDGDGDLDLYVV
                     NYGTPNKLFRNDGPSGFVDATAGPLADAGWGLAACWGDYDNDGDPDLYLTNDGPNRLL
                     RNDGNGVFTRIAGVYIEDGGPGQGAAWGDYDNDGDLDLYVANYGTPNKLIRNDGGNVF
                     TWITAGPLGDTSNSTGVAWGDYDNDGDLDLYVANYGQPGKLLRNDGGGVFTALTGGPL
                     GDAGNATGTAWADYDGDGDLDLYVANDATGSRLLRNDLASSNHWLELTLVGRISSRDA
                     IGARVRVATGSLKQIREVSGGSGYMSQDSP"
BASE COUNT         1539 a         3167 c         3083 g         1458 t
ORIGIN      
        1 tcgcgcgccg cctcgagcag ctcgcggagc ggccccaccc gcagttccgg cacctcggtc
       61 agcgggtcct tcccctcgcg gaactgcacg gtggccaggt cggcgtggcg gcgcgacttc
      121 tcgagctgat cggcggcgag cgaggcgcgc tcgcgctcgt gcaccagctc ttccgaggcg
      181 gcgcggcgtg tctcgacgct gcccgagagg agggcaacca cgacggcccc gaagatcgcg
      241 gccaccacgg cggcggcaaa acccatcgcg ccgggcagga agtgtccgac cagtcccgcc
      301 accagggcgg cgatgaacgc ggcgatcgag aacggcggcg cggaggcggc cggcgcgggg
      361 tgggaggccg gcggcgcggt ctcatgcagg gacgtcacgc gcggtgtcgg agctgcggac
      421 gattcctcgt gcggcgccgg cttgaaaggc ggatgcgtct cctgcgctgc agcgcggcgg
      481 cctttggcct cagcggtcat cgtcacctct cctcgatcga atcggaatcg gaacccgccg
      541 cgaccggagg catcacggcg tcgaaaatgg accagcagcg gaggcagtgg atccgctccg
      601 cgccggcgag atacaccgag ccgatgaccg cggccagctc tccgtcggcg atcgactcgt
      661 cggactcctt gatctcacgc cctggccaca gccggggcat cgacggaatc aggagggcga
      721 agcgttcggt cccgcgcgac agcaccacgc cgtagcgcgg cgcatcgccc gtgggtcccg
      781 acacgccgag ccattcgcag agatccacga ccggcaccat cgtgccccga aacgccgtca
      841 cgcccgagac cgcgccgctt cggcgcggta gcggcgcgat gtgatcggct tcgatcaaac
      901 cagccacgtc ctcgaccgga acgccgaaga gaatctcgcc gaccggaaac gcgatgaggc
      961 cgcgcgtgcc cagggccgtg cccgattcgc gtcgcaatcc acctccgtat gtaggacggc
     1021 tgtcactgct gcgaaaaggc aaagagcgtg ccgcggtgat ggctcggcgt cgcgtcgcaa
     1081 gtcgctgccg cgcttcgcgt cggagcgccc gtccggagcg tcgcgtaaag ccgagaccaa
     1141 tttccgtgcg cctcgcttcg tgcgtgtcgc aattccttcc gcaattcgca acccgcccac
     1201 tcatcgtcgc agcgggtggt tacacccgac ccttcggctg aaagaggcca catctggagt
     1261 caaaacgtcg cgcctcgggg aggtgtcgtc tttccttacg gtttacgaat cgattgcggc
     1321 ggagagggtt gcggccgtat gaccctcccg cctcctattc ggcacggagg gtgcataaca
     1381 catcgtcgtc acgcggcgag gccgcgcggc aggaagtgac caaagagtgg ggtggtccgg
     1441 agagggaggc gagccatgac ggcagcccgt aagattctag cgagtaagac gaatcccagg
     1501 aaggttcggc cgaccgcgta tgtggtgatg aactgcctcc tcctgacgaa ggcggaaggc
     1561 acgccgcaga acggcgaccc gcttcctcag gaggacgtcg acgggtatct cgccgcgctc
     1621 aaggatcagc tcgaggcgca ggtgccgttg ccgccggagc gtagccggct cctttcacgg
     1681 tacgacgtgg cggcgtggcg gcggctggcg agcgcgcatc gcggctctct tcgctacctc
     1741 atctatcgaa cgccggagga tctcctgctc atctcggtag gatccttcga gcagaccggg
     1801 ccgtacgtcc cgaaccggcg cgaggggttg ctgccggggg aagaggcgga cgtcggccgg
     1861 agcccgaact ggtaccactt ctcatacacg tacggtgagc tcctcgagaa ccccgggcag
     1921 acgctgagcg cggtgctcga gaagctgacg gtgagcctgg agcgctacaa caagatgctc
     1981 tcgcacatgc gcggcgaaca ctggagtctc gccaagcggt tcaagaaggc gcaggtgacg
     2041 aggctctcgc gaacggtgga cgaggacgcg cgcgacgaat cgctgcgcgt gatgcaggac
     2101 cggctcctcg acgcctacgg cgagtggaag cgcaccggcg agcgccggca cctggacgag
     2161 atccggggcc tggcccgcga gatccggctc ctcgcacccg acttccagtt ctcgctcccg
     2221 agcggaggcg acgcctaagc gccccgacag agagcggcaa gatcccccgc gcgcccttcg
     2281 gttcccccga cccgaacact ccggagggcg cgcatcccca tgtcagcccg gcccgcaggc
     2341 cgggctgact cctgttcagt agaaatgaag cctcgcagct tcaggtgagc aacctcagtc
     2401 gagtcgatac aacacgtcgg gtagtcccac ctgattcccc gtcccatcac caaacttggc
     2461 taccgagaac ccccgcgcct catagaactc gcgagcccgc tcattgcttt gaaacgcata
     2521 caaccacacc ggcgcccgca gaatcgcaag cgcatgctct aacaacgcgg tcccgactcc
     2581 gcgtcccaca gactccggcg ccacgtacag atggtcgatc catcctccct cgccgtcctc
     2641 ggagatggcc atcatcgcga tcaccgcatc attctcggtc gcgacgaaga cgcgcttctg
     2701 caccatgacg cttttcgcga tccaggcgcg gacctggtcg tcggtgtggc cgatggggat
     2761 gtcgggcatc agggttttcc aggagtggag gaagacgttg gcaatggcgt cggattcgtt
     2821 ggggcgggcg gggcggatgg tgggggccat gaattggaaa ctgcgggcgg gttagacgaa
     2881 agaacaggag ttagtccggc tgcggccgga ctgacatcta acgtatttcg taattcggcg
     2941 cttccttcgt aatcgtcaaa tcatgcggat ggctctcgcg gaggccggac tgcgtgatgc
     3001 ggacgaagcg ggtctgggtg cggagtccct cgatggtgcc gacgccacaa tagcccatcc
     3061 cggcacgcag gccgccgatg agctggtaga tcacgttggc gaccggaccc ttgtagggca
     3121 cgcgggcctc gattccctcg gcgaccagct tcttgagatc gcggacgccc tcctggaagt
     3181 agcgatcgcc gcggccggag gacatcgcgg acagggagcc catacctcga tagaccttat
     3241 aggtcctgcc ttccaggagc agcgtctccc cgggactctc ctcggtgccg gcgagcaggt
     3301 ttccgagcat caccgagtgc gcccccgcgg ccagcgcctt caccaggtcg cccgagtact
     3361 tgatgccgcc gtcggcgatg atcgggatgc cggccttcgc cgcttcgttg gcgcactcga
     3421 gcaccgcgct gatctgcggc acgcccacgc cagccactac gcgcgtcgtg cacgcggagc
     3481 cgggacccat ccccaccttc acgcagtcgg cgcccgcccg gatcaggtcg cgcgccccgt
     3541 cggcggtggc cacgttcccc acgatgagct ggagattggg atgggcgttc ttcacggcgc
     3601 gcgtcgtctc gatcacggcc ttggagtggc cgtgggcgct gtcgatcacg accacgtcca
     3661 cgcccgcgcg aaccagctcg gccacccgct cgttcgcgtc ctggccgacg ccgatcgcgg
     3721 cgcccacgcg gagccggccc agatggtcct tgcaggagcg cgggtagcgg attcgcttct
     3781 ggatgtcctt cacggtgatc aggccgcgga ggctgtactg gtcgtccacc accgggagct
     3841 tctcgatctt gtgcgtgtgc aggatccgct cggcttcctc gagggtcgtg cccacgggcg
     3901 ccgtgacgag gttctccttg gtcatcacgt cgcgcacctt gagatcgggg ctcttcacga
     3961 agcggagatc gcggttggtg aggatgccga cgagccgctt cccttcggtg atcgggacgc
     4021 cggagatccg gaatttctcc atcagctcga gcgcgcgggc gatcggcacg tcgggcggca
     4081 acgtgatcgg attcacgatc atgccgctct ccgagcgctt gaccttgtcc acttcctcca
     4141 cctgctcgag caccgggagg ttcttgtgga tgatccccag gccgccctcg cgcgcgatgg
     4201 cgatggcgag cgcgctctcg gtcacggtgt ccatcgccgc gctgaccacg ggggtgttga
     4261 gcgtgatctc gcgggtgagg agggtgctgg tgtcgacgtc gcgggggtgg acttcggagt
     4321 agccgggaag caggaggacg tcgtcgaacg tgagcgcgta gtccaccggc atgcgctccg
     4381 gcgcggtctc cggccgcacg ctgggtgcga acttctcgcc gggcgccgcg aaggtcctca
     4441 tacgttactc cggtagtccg gtatcgatcc gctccgtgag cgtgaagtag aacgtgacgc
     4501 ccaggcggat ctgcgcgtag ctcttgttcc cattataacc gctcggaaag tcggcggtgt
     4561 tctgggcaaa gatgtggtgg tacacggcgt cggcggtcgt gctgatgtgc ttggacatcg
     4621 tccgctccac cccgaccatc ccgtagaagc cgggatcggc ccgccggaac cgctcgaacg
     4681 tgctcgggtc cttggcggcc ccgaggtcct tggactggat cgaccagacg tagagcccgc
     4741 ctccggctcc cacgtacgga cgcatcgtcc tcccttcgtg aagcaggtag cggagcccgc
     4801 ccgtgatcgg caccgcgctc tccagccaga accgattgtc gtcggtatcg agccggttcc
     4861 acgcgtagcc caccgtcacg tcgccccaga cgtggctgcc gaagacgtag gagaagatca
     4921 ggtcccccga gaagcgcggc ttggtgccgt cggagagcgg ctggttgttg gtcaggctga
     4981 gcggcgtgtt cgcctcgctg ccgaacttgg acagcccgcc acggaagccg atcccgccga
     5041 agccggtgag atcgtccgcg gttgccgtca gaggtaacgc gatgaccagg agggccagca
     5101 gccctcccac gacagttcga gacatgagtg ttggaagctc ccttgggtgg cgggcggggt
     5161 cacccggcca gccggcatgt ggtcgtggtg acggcagttt aggggagccc acgagacgtg
     5221 tcaatcgcgc cccccgccgc gtcgaagcgc gtgcgcgggc ttcgaacggt cgcggcgcgg
     5281 cgccggccgc cttgacagaa accggggccc ccgcctactg ttgctgcccc gaggtggcgg
     5341 tgtagctcag cggtagagca ggggaatcat aatcccttgg tccggggttc gaatccctgc
     5401 gccgccacca gtttgcccgc agtgccccaa tgacactctg acagtgacac ccgccatgct
     5461 gtcaggagac ctccgcgtca cagttcccgt gttaagggcc tcttaaccga atccccaacg
     5521 acttagccca gcttcggtgc tggcacgcct cgtgccgtcc atcccgtctg aatcccgatc
     5581 gcaaatcccc ccgcgggctc gcgcgaacgt tccgcgagag gtgtatgggg gcggaacaat
     5641 cgtgcggcct gacaggtcgc ggaagggatt cctgtttgag gggacgtgca gccggctgcc
     5701 cccggtcgtc tgcgcgtgca gggctccgag gtgcggcgac gggagccctc gcgtgctcaa
     5761 ccgccgcggg aacatgcgcc tggcgcatgg cagtggaggt ctcgtgaggc tgcatccatg
     5821 catcaagccg gtgttgcgct ggagcggggt cgccttgacg gcggcgatca tcctggtcct
     5881 cggagcgtcg gctggagccc aatccgttct cgatccgctg tccctggcca agtacgtcga
     5941 ccctctgccg attccggccg cgatcgacgc gaccatgccg ggcagctcct tcgagatcgg
     6001 cgcctaccag atccagcaga agcttcaccg cgacctgccg ctcaccacgg tgtacggcta
     6061 cggcctgacc gaagcgacgg cgacctatcc gggcccgacg atcgtggcgc ggcacaacgt
     6121 gccgatccag atccggtgga cgaaccatct cccgatgacg cacatcctgg actacgcgat
     6181 cgacccgacc ctcatgaggg ccgagacctc gaccggcgtg ccgatcacga cgcacgttca
     6241 cggcgccgag gtcgagccgc agagtgacgg cggtccgaac acctggttca cgaaagattt
     6301 cgcggagacg gggcccgact tcacgagcca agtgcacacg tacgtcaaca cgcagctcgc
     6361 gtcgacgatc tggtaccacg accacgcgtt cggctacacg cggcacaacg tgtacgcggg
     6421 actcgcgggc tactacctga tcaccgatcc cggcaatgag ccgccggggc tgcccgaggc
     6481 tccctacgac atgggcctcg cgatccagga ccgcatgttc accaccgacg gccagctgtg
     6541 gtatccgaac gagggggtga cctccgttca tccgaagtgg attcccgagt tcttcgggaa
     6601 cgtcatcctg gtcaacggaa aggtgtggcc ctacctgaac gtcgagccga ggaagtaccg
     6661 cttccgcatg ctcaacggct cgcaggcgcg cttctacagc ctggctctgc tggagcgggt
     6721 gggcagcaca ccgggaccgg ccttctacca gatcgggacg gacggcggct atctcgccga
     6781 gccggtgctc ctgaacgacc cggccaacgt ccactcgccg cggctcgtga tcgcgccggg
     6841 cgagcgcgcc gacgtggtga tcgacttctc ggcctacgcg ccgggaaccg agttcctcct
     6901 gcgcaacacg gcgaaagctc cctatccgat gggtgacgcg ccggacggac agaccaccgg
     6961 acagatcatg ctcttccggg tggttccctc cacgggtccg gacaacagcg tgatcccgcc
     7021 ggcgctcgcc acggtgaacc gcctcaccaa tccgacggtg acgcgcacga tgacgctgaa
     7081 cgagaggctg ctgggcgatg atccgctcgg cgcgctcctg aacgggatgc cctacgacgc
     7141 gcccgcgacc gagtatccgg tgctgggcac gaccgagatg tgggagatcg cgaacctgac
     7201 cggcgacacg catccgatcc acctccacct ggtgcagttc cagctgctga accgccagaa
     7261 gctgaacgcc cgccgctacc agatggcgtt cgacgcggcg aatccaacac ttccctccca
     7321 aacgtacgtc cccgtgccgg tggggcccta tctcaagggc aagccgacgc cggccgacgc
     7381 gaacgagcgc gggtggaagg acacgttccg gatgaacccg ggcgaggtca cgcgcgtgct
     7441 ggtgcgctgg gcgccgcagg atgaatcgcc gtcgttcgcg ttcgacgcga ccgcggaccc
     7501 gggatacgtc tggcactgcc acattctcga gcacgaagag aacgacatga tgcggccctt
     7561 ccacgtcgtc gctccgggcg cggcgacggc gggcggcgct tcggtcaacg cggctccggc
     7621 gttcgcgccg gcttccctgc ccggcggcac ggtcgcgttc cgcgcttcga accccgcgct
     7681 ggaaggctcg aatctccggt tcagcctgac gcgcgcggga tcggtggccc tggatctctt
     7741 ctcggtcgcg ggacggcacg tcgccacgct ggcctccggc tggtacgagg cgggcgacca
     7801 cgacgtctcc tggacggcgc gcgccacgtc gggcgagccg ctcgcgaacg gcgtctatgt
     7861 cgtgcggttc cgcggcgacg gcgtcgagcg cgcgcagaag atggtgctgg tgcggtgatt
     7921 ctcatgggga ggccccgccg gcgcggggcc tccgcttctc cccgacgcac gagcgcacga
     7981 cgatgaaccc acgcttcgcc cggacttccg ctctcttcct ctccgcggcc gccgttctcg
     8041 tcgcggcgcc ggcctcggct caattcaccg cgctcacgaa ggcaccgctc ggcgaccggg
     8101 gctccgcgtt cggcgtggcc tgggccgact acgacctgga tggcgacctc gatctctacc
     8161 tcacggagga cggcccgaac ctcctgctcc ggaacgacgg cggcgacaac ttcacggacg
     8221 tgacctcgcc tccgctggac gagggaggaa atgggagcgc cgcggtgtgg ggcgactacg
     8281 acggcgacgg cgaccccgac ctctacctcg tgaacttcaa ctcgccgaac catctctatc
     8341 gaaacgaccg cgccgccggg ttcaccgacg tcacggccgg gccgctcggg acgaacggcc
     8401 ccggccagag cgccgcctgg gccgacgtcg atggcgacgg cgacctcgac ctgtacgtcg
     8461 tgaactacgg aacgccgaac aagctgttcc ggaacgacgg cccgtcgggt ttcgtggacg
     8521 cgaccgcggg cccgctggcg gacgccgggt gggggctcgc cgcatgctgg ggcgactacg
     8581 acaacgacgg cgatccggac ctctacctca ccaacgacgg tccgaaccgg ctcctgcgca
     8641 acgacggcaa cggggtcttc acccggatcg ccggcgtcta tatcgaggac ggcggcccgg
     8701 gtcagggcgc ggcgtggggg gactacgaca acgacggcga cctcgacctc tacgtcgcca
     8761 actacggcac cccgaacaag ctcattcgca acgacggcgg gaacgtgttc acctggatca
     8821 cggcggggcc cctgggcgac acgagcaaca gcaccggcgt ggcgtggggg gactacgaca
     8881 acgacggcga cctggatctc tacgtcgcca actacggcca gcccgggaaa ctgctgcgca
     8941 acgacggcgg cggcgtcttc acggcgctca cgggcgggcc gctgggcgac gccgggaacg
     9001 cgaccggaac ggcctgggcc gactacgacg gagacggcga cctcgatctc tacgtcgcca
     9061 acgacgccac ggggagccgc ctcctccgga acgacctcgc ctcctcgaat cactggctgg
     9121 agctgaccct ggtcggtcgc atctccagcc gggacgcgat cggcgctcgc gtgcgcgtcg
     9181 ccacgggctc gctgaagcag atccgcgagg tctccggagg atcgggctac atgtcgcagg
     9241 actctcc
//
LOCUS       DASSLZ010000215         4037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1706200, whole genome shotgun sequence.
ACCESSION   DASSLZ010000215 DASSLZ010000000
VERSION     DASSLZ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706200"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1250
                     /gene="mnmA"
                     /locus_tag="VFM00_08305"
     CDS             144..1250
                     /gene="mnmA"
                     /locus_tag="VFM00_08305"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET7498287.1"
                     /translation="MKPDLPASKRTVLVAMSGGVDSCVAAALLHRAGHTVIGVTMKLW
                     CYAEGPSPARGCCTLDAIDDCRAVARKIGFPHYVLDLEDDFREHVIEDFVGEYLAGRT
                     PNPCVQCNNWLKFGELMRRADLLECDLVATGHYARRGLDADGRATLLRAVDGTKDQSY
                     VLWGLTQAAISRALFPLGTMTKEEVRKAARELGLSVADKKESQDICFVEGKRYVDFLR
                     QRYPERLAASPAGEVRDAAGRALGRHDGVHGFTVGQRRGVNVAAGRRLYVTRIEAGTG
                     TVVVEEEAALYHREARIGRVNYLSGTVPESTIEGAAKIRYLHAPAPASLTPSDPDRAE
                     VRFQEPQRAMTPGQSLVLYEGDRVVAGGIIQDVS"
     gene            1240..3369
                     /locus_tag="VFM00_08310"
     CDS             1240..3369
                     /locus_tag="VFM00_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7498288.1"
                     /translation="MSPDPEAPAGAPGTAPRHTNRLAAEKSPYLLQHAHNPVDWFPWG
                     DEAFAKARAEDRPIFLSIGYATCHWCHVMERESFESVEIASILNSSYVPVKVDREERP
                     DVDHVYMTVCQALTGSGGWPLTVILTPDGRPFLAGTYFPPESRYGRPGLKDVLYQVLS
                     AWEGQRHRVEDVAGQIVEAVRGEFAGMPGERPEPGLLTKAFEHLHQRFDEEFGGFGTA
                     PKFPTPHQLTFLLRYWKRTGDGRALDMVERTLRWIRRGGVYDQLGFGAHRYSTDREWL
                     VPHFEKMLYDQALLLMAYAEAHRATGDPLYASVAREIAAYVARDLTSPEGAFYSAEDA
                     DSEGEEGKFYVWTMEELVAALGPEEAALFARVYGAETKGNWVDPAGGHLTGTNILHLT
                     EDPGAVARETGLDAAAMARRLEADRALLLEARSKRPRPLRDDKVLTAWNGLMIAALAK
                     ASVALEEPRFAEAAERAIDFLERHLLRADGRLFARWREGEAAHPGYLDDYAFLAWGSL
                     ELYEATFDPRHLERALALAGDMERLFADEKEGGFFFTGSDAAPLLSRTKEIYDGAIPS
                     GNSVAVLVLLRLSRMTGRSGLERRAEETVRAFGGSVARQPGAHTQLLSALDFAFGPTR
                     EVVIAGPAEAPGTVALVRTARSAYFPRTVFLLREPGEAGEALVRLAPFTKAQLPVNGV
                     PAAYVCENFACAAPVSDPSALASALAT"
     gene            complement(3329..>4037)
                     /locus_tag="VFM00_08315"
     CDS             complement(3329..>4037)
                     /locus_tag="VFM00_08315"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7498289.1"
                     /translation="LASLKEGLRRKEPVTMAMTEAGYGSSSRLYERSPEALGMTPGDY
                     RAGGPGAKIAYTVAKSPIGALLVAATERGVCSLKIGGEDASLARSLREEFPAAELKRD
                     ETALGRWVRAIVNHLSGKETRIDLPLDIRATAFQQRVWEALRAIPYGETRTYQEIARI
                     VGAPKAARAVGHACATNPVAILIPCHRVVRGDGTLGGYAYGLHVKRKLIDTELARRER
                     AKGRVTSREPKRARSGR"
BASE COUNT          565 a         1434 c         1441 g          597 t
ORIGIN      
        1 cgtcgcccag gcggccgtgc gctggagcgt cggccgcgcc acgaccgaag ccgagatcga
       61 ctacgttctc gagacggtcc ccgccgtcgt ccagaagctc cgcgccgcct cgcccacgtt
      121 cgcggcctga ggcggccctc cggatgaagc ccgacctccc cgcgtcgaag cgcaccgtcc
      181 tcgtcgccat gagcggcggg gtggactcgt gcgtggccgc ggccctcctc caccgggcgg
      241 ggcacaccgt catcggcgtc accatgaagc tctggtgcta cgccgagggg ccttcccccg
      301 cgcgcggctg ctgcacgctg gacgcgatcg acgactgccg cgcggtcgcg cggaagatcg
      361 gcttccccca ctacgtgctc gacctggagg acgatttccg ggagcacgtg atcgaggact
      421 tcgtggggga gtacctggcg gggcgaacgc ccaatccctg cgtgcagtgc aacaactggc
      481 tcaagttcgg cgagctgatg cgtcgtgcgg acctgctcga atgcgatctc gtggctacgg
      541 ggcactacgc gcgccgcggc ctggacgccg acgggcgcgc cacgctcctg cgcgcggtcg
      601 acggcaccaa ggaccagtcg tacgtgctct ggggcctcac gcaggcggcg atctcccgtg
      661 cgctctttcc cctggggacg atgaccaagg aagaagtgcg caaggccgcg cgcgagctcg
      721 ggctttcggt cgccgacaag aaggagagcc aggacatctg cttcgtcgag gggaagcgct
      781 acgtggactt cctgcggcag cgctatcccg agcgcctggc cgcctccccg gcgggcgagg
      841 tgagggatgc cgcgggccgc gcgctcggcc gccacgacgg cgtgcacggg ttcacggtcg
      901 ggcagcgccg cggcgtgaac gtcgccgcgg ggcgccgtct ctacgtcaca cgcatcgagg
      961 ccggcacggg gaccgtcgtc gtggaggaag aggcggccct ctaccatcgc gaggcgcgga
     1021 tcggccgggt gaactatctc tccggcacgg tcccggagag cacgatcgag ggcgcggcca
     1081 agatccgcta cctccacgcg cccgctccgg cctcgctcac gccgtcggac ccggaccgcg
     1141 cggaagtccg tttccaggag ccgcaacggg ccatgacccc ggggcagtcg ctcgtgctct
     1201 acgaaggcga tcgcgtcgtg gcgggaggga tcatccagga tgtctcctga tccggaagcg
     1261 ccggcgggcg cgcccggcac ggcgccgcgg catacgaacc ggctcgccgc cgagaagagc
     1321 ccctacctgc tgcagcacgc ccacaacccc gtcgactggt ttccctgggg cgacgaggcg
     1381 ttcgcgaaag cgcgcgccga ggaccggccg atcttcctct cgatcggcta cgcgacctgc
     1441 cactggtgcc acgtgatgga gcgcgagtcg ttcgagagcg tggagatcgc gtcgatcctg
     1501 aattccagct acgtgccggt caaggtggac cgcgaggagc gtcccgacgt ggaccacgtc
     1561 tacatgaccg tctgccaggc gctcacgggc tcggggggat ggcccctgac cgtcatcctc
     1621 acgccggacg gccgtccgtt cctcgcaggg acctattttc cgcccgagtc acgctacgga
     1681 cggcccgggc tgaaagacgt gctctaccag gtgctgtcgg cgtgggaagg gcagcggcat
     1741 cgcgtcgagg acgtcgcggg gcagatcgtg gaggcggtgc gcggcgagtt cgccggaatg
     1801 ccgggtgagc gtcccgagcc gggcctgctc accaaggcct tcgagcacct gcaccagcgc
     1861 ttcgacgagg agttcggcgg cttcggcacc gcgcccaagt tcccgacgcc gcaccagctc
     1921 acgttcctgc tgcggtactg gaagcgcacg ggcgacggac gcgcgctgga catggtggag
     1981 cggacgctcc gctggatccg ccgcggcggc gtctacgacc agctgggctt cggcgcgcac
     2041 cgctactcga ccgaccggga atggctggtg ccgcacttcg agaagatgct ctatgaccag
     2101 gcgcttctcc tgatggcgta cgccgaggcg catcgggcca cgggcgatcc cctctacgcg
     2161 tcggtcgcgc gggagatcgc ggcctacgtg gcgcgcgatc tcacgtcccc cgagggcgct
     2221 ttctactccg ccgaggacgc cgacagcgaa ggggaagagg ggaagttcta cgtctggacg
     2281 atggaggagc tggtggcggc gctgggcccc gaggaggcgg cgctcttcgc gcgcgtctac
     2341 ggggccgaga cgaaggggaa ctgggtcgat cccgcgggag ggcacctgac cgggacgaac
     2401 atcctccacc tcaccgagga tccgggcgcg gtggcgcggg agacgggact ggatgccgcc
     2461 gccatggcgc ggcgtctgga agccgaccgc gccctgctcc tcgaggcgcg ctccaagcgc
     2521 ccgcggccgc tccgcgacga caaggtgctc accgcctgga acgggctcat gatcgcggcg
     2581 ctcgcgaagg cttcggtcgc gctcgaggag ccgcgtttcg ccgaggcggc cgagcgcgcg
     2641 atcgatttcc tggagcggca cctcctgcgc gccgacggcc ggctcttcgc gcgctggcgc
     2701 gaaggggagg cggcgcatcc cggatatctc gacgactacg ccttcctcgc ctggggatcg
     2761 ctggagctgt acgaggcgac cttcgatccg cgccacctgg agcgcgcgct ggcgctggcg
     2821 ggcgacatgg agcgcctgtt cgcggacgag aaggaggggg gcttcttctt cacggggtcg
     2881 gacgcggcgc cgctgctttc gcgcaccaag gagatctacg acggggcgat cccttcgggg
     2941 aactcggtcg cggttctcgt gctgctccgc ctctcccgca tgaccggccg ctccgggctg
     3001 gagcggcgcg ccgaggagac ggtgcgcgcg ttcgggggct cggtcgcgcg ccaaccggga
     3061 gcgcacacgc agctcctctc ggcgctcgat ttcgcgttcg gcccgacgcg tgaggtggtg
     3121 atcgcgggcc ccgcagaggc gcccgggacc gtggcgctcg tgcgcaccgc gcggtcggca
     3181 tacttcccgc gcaccgtgtt cctgctccgc gagccgggag aagcagggga ggcgctggtg
     3241 cggctcgccc ccttcacgaa agcccagctt ccggtgaacg gggttccggc cgcctacgtc
     3301 tgcgagaact tcgcctgcgc cgcgccggtc agcgacccga gcgcgctcgc ttcggctctc
     3361 gcgacgtaac ccgccctttc gcgcgctcgc gccgcgcgag ctccgtatcg atcaacttcc
     3421 gcttcacatg gagcccgtag gcgtagcccc ccagcgtgcc gtccccgcgc accacccgat
     3481 ggcaggggat caggatcgcg acgggattgg tggcgcaggc atgcccgacg gcgcgcgccg
     3541 ccttgggcgc tccgacgatc cgcgcgatct cctggtaggt ccgggtctcg ccgtacggga
     3601 tggcgcgcag cgcctcccac acgcgctgct ggaacgccgt ggcgcgaatg tccagcggca
     3661 ggtcgatccg ggtctccttg cccgagaggt gattcacgat cgcgcgcacc cagcgcccga
     3721 gtgctgtctc gtcgcgcttg agctcggcgg ccgggaattc ctcgcgcagc gatcgcgcga
     3781 gcgaggcgtc ctcgccgccg atcttgaggc tgcacacgcc gcgctcggtg gccgccacca
     3841 acagcgctcc gatcggcgac ttggccaccg tgtaggcgat cttcgccccg gggccgccgg
     3901 cgcggtagtc gccgggggtc atccccagcg cctcgggaga acgctcgtag aggcggctgc
     3961 tcgatccgta tcccgcttcc gtcatggcca tcgtgaccgg ctcctttcga cgcagccctt
     4021 ccttgagcga cgccagc
//
LOCUS       DASSLZ010000216         7580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1840774, whole genome shotgun sequence.
ACCESSION   DASSLZ010000216 DASSLZ010000000
VERSION     DASSLZ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840774"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1478)
                     /gene="gltX"
                     /locus_tag="VFM00_08320"
     CDS             complement(6..1478)
                     /gene="gltX"
                     /locus_tag="VFM00_08320"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006721318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET7498290.1"
                     /translation="MTAPVRVRFAPSPTGYLHVGGARTALFNFLFARRHGGVFILRIE
                     DTDRERSTKEAVDAIFEGMRWLGLTWDEGPDKGGDFGPYFQSQRLALYAAAAEKLLVE
                     EKAYRCFCDPEALKARREVALARSEPPKYDRTCHRLTREESAARAAKGEPHALRFFMP
                     EGMTTWDDSVRGTVTFQNATLDDLVILRTDRHPTYNFAAVVDDSAMRISHVLRGDDHI
                     SNTPRQIQIYKALGLETPVFGHVPMILGPDGTRLSKRHGATSVTEYRDAGFLPEAMVN
                     FLALLGWAYDGEREIFTLKELTEVFSLERISKNPAIFNHEKLEWMNGEYFRALPIATR
                     ADLVAEHLRATGGLTESALRDRPQLERAVAAVGERMRRPADFLTYAAFAFVDELEPEP
                     AAWADLVAKTGAGERLKRLADVLATVEPFEHDPIEQAVRGLAAELGVKAGEVIMPARI
                     ALTGKKVTPGIFDVILLLGRDRAVARLRRAAERLESPAPA"
     gene            complement(1540..5706)
                     /locus_tag="VFM00_08325"
     CDS             complement(1540..5706)
                     /locus_tag="VFM00_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HET7498291.1"
                     /translation="MTAVSVSSFTNCRTLLLLLLLIVASAFTAVGAEGALPPRTTRLF
                     GQPGRAAPFAPTLLPSTSGSPVDCVIVTPDSLADIFQRLADYQTRIGRPTVVRGISTI
                     RAADPRSNDLPQAIRSFLRSARDLWGIRWAILAGDHDTVPLRMVRVTFATVEDIPTDA
                     YYADLDGTWDANGNGIYGETGDGLDMRPDIAVGRLSAATRADAKALVDKALRYAGAPF
                     LPMLAKQLVLAEVLLPQNWTPGAPVQVDGAAEGQALTAGAPGCAVVDRYYENDTAYPG
                     AAHLNRANALSALSRGYNAVFQIGHGARSQLSVGSELLTLGDLAAITNGDSAALWIAT
                     NCASAGVDYDCVAERLTRRPDGGAFAYVGATRDAWPSPAAAFTEDLAARLFHDPVPAS
                     GITLGEAMEDARAALLPAASTETPDRWTYFESVLIGVPSLPIWRCPPSSFAVTAPASM
                     PLSGRSIAVTVMAGGTPAESALVVAWKAGEDYVTALTNAAGQATLPFHPSVTGRFSLA
                     VTKDGVRPYLDSLNVTADAPAHFAALGVAPTDAVVGDGNGLIEAGESFRVAGAVKNTG
                     GAASSGPVTLEIKAASGPVVVDKATAVVGALAAGAQLTIPDSLLAHALASPNAPGIAR
                     LRFIARDAARADTADVDVEVAAPSLLFTKATMTDTNGNGVLEAGENATFTWTLGNEGD
                     GRARAPSLLAINPGSGISFVTPSGSAPSLAPGGTAATTAIVVHAATAPPARLFDLRIT
                     DAYGHVWTLPVERVVPPAPTGLRAPASGVAGVTLSWDAVAAPDLLGYVVLRSGADTTA
                     APVEASPLPVRRTSAFENAGLAPLTAYWFAVAAVDSSGNRSARTPWLLVSTTPASVSG
                     WPAPLAAATSASVCLADLDGDGRVEVITGADQLYAFHADGSEWRDGDANPVTLGVFST
                     LLHNIASTPAAADVNLDGTPEIIAASWNDSLVAVFRANGTLLPGWPKKGAAPFWSSPA
                     VGDLDGDSAPDIVIGSNTSRLYAWRADGTELRDGDGNPATDGVYFVPVGTVISSPAIA
                     DLDADGRREVVFGTSAGRVYALRDGVPLSGWPFVANGLMSSSPAIGDVIAGGGLETAM
                     ACANDSLYLITAAGQRAPGWPRPLELTPGNGRVPSPVLAPLRRHLGDPSLDVIVCGAD
                     GRVRAYDGAGAILPGWSVVQLGAGSEASPAVADLNGDGALEVLIGAEDRRLYAFHFDG
                     TPVSGFPIEIGAEVRSTPAVWDLDGDGACEIALTGWDGAVHVWRYPGSFASVGMAWPM
                     FHHDNWHTGVASFPILTSADPQPVPDPAPSPEARASLAQNRPNPFNPVTVIGFRVPGP
                     AAADVTLRIYAADGRLVRTLVSRRFDPGYHEARWDGAGDSGAPLASGVYFYRAEIGGS
                     AFTRKMALLR"
     gene            complement(5764..7356)
                     /locus_tag="VFM00_08330"
     CDS             complement(5764..7356)
                     /locus_tag="VFM00_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498292.1"
                     /translation="MSDGPASPWRARHHAALGLLALLALALLLPFRGYLTDDTFIHLQ
                     FAKHLIRGEGFAFNAGEPTYGATSPLWVLLLAGTGAFLHGAGATPASADSMPPLAAAA
                     KVWGTLFTAASVLLMAAIARALRWEPRWALAASAMVALDAWSVRWAISGMETPLALAL
                     ALLACLLLAHALLHGRSPAWAGLALGAAFLARPEFLLLFMLSLAAVAWAAEPRARVGR
                     VAALAGGAALTAGPWLALAWMWFHRLLPNTGAAKAAAWLDPGRAASALRESIRALLAA
                     DLVPVAIAVLALAWLHPWSAMGKARGRRAWWGLVLAWPVLLVLAFAAEGVQVVSRYLV
                     PAMPAVLLIGLAALRAASRVWAPRRRAAALAAALLLFAAQNLYVTLAVSAPHARRHTQ
                     GLRDSLASLGLWARAHTAPGTLFAVPDIGAFGYYSDRPVLDLYGLVTPGMARASVRDG
                     YDAVVWNLLYEPVGRPAYLIDRSTREGRLAPPDDPATPYRFLFSRSIPDLGITRPTPY
                     VYSVYAIDWTIYDRLHPRMAEE"
     gene            complement(7384..>7580)
                     /locus_tag="VFM00_08335"
     CDS             complement(7384..>7580)
                     /locus_tag="VFM00_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498293.1"
                     /translation="PDGVATVVIEAARTEPWAAIVGKDALRIGLSRYGASAPAEVIAK
                     ELGFTPDAVAARVARWLERT"
BASE COUNT         1131 a         2678 c         2819 g          952 t
ORIGIN      
        1 ttccgtcacg ccggagcggg cgactcgagg cgctcggcgg cgcggcggag ccgcgccacg
       61 gcgcggtccc gtccgaggag gaggatcacg tcgaagatcc ccggagtcac cttcttcccc
      121 gtgagcgcga tccgcgcggg catgatcacc tcgcccgcct tcacgccgag ctcggccgcg
      181 agcccgcgca ccgcctgctc gatcggatcg tgctcgaagg gctccaccgt cgcgagcacg
      241 tcggccagtc tcttgagccg ctcccccgcg cccgtcttgg cgacgaggtc ggcccaggcc
      301 gccggctccg gctccagctc gtcgacgaac gcgaacgccg cgtaggtcag gaagtcggcg
      361 ggacgccgca tccgctcgcc gacggccgcg accgcgcgct cgagctgcgg gcggtcccgg
      421 agcgccgatt ccgtcagtcc gcccgtcgcg cgcaggtgct cggccacgag gtcggctcgc
      481 gtggcgatgg gaagcgcgcg gaagtactcg ccgttcatcc actccagctt ctcgtggttg
      541 aagatggcgg ggttcttgga gatccgctcc agggagaaga cctccgtcag ctccttcagc
      601 gtgaagatct cgcgctcgcc gtcgtaggcc cagcccagga gcgccaggaa gttgaccatg
      661 gcctcgggga ggaaccccgc gtcgcgatac tcggtgaccg aggtcgcgcc gtgccgcttg
      721 gagagccgcg tgccgtcggg ccccaggatc atcgggacat ggccgaacac cggcgtctcg
      781 aggccgaggg ctttgtagat ctggatctgc cgcggcgtgt tcgagatgtg gtcgtccccg
      841 cggagcacgt gcgagatccg catcgccgaa tcgtccacga cggcggcgaa gttgtacgtc
      901 gggtgccggt cggtgcgcag gatcaccagg tcgtccagcg tcgcgttctg gaaggtcacc
      961 gtgccgcgca ccgagtcgtc ccaggtcgtc atcccctcgg gcatgaagaa acggagcgcg
     1021 tgcggctccc ctttcgcggc gcgcgccgcg gactcctcgc gcgtcagccg gtggcaggtg
     1081 cgatcgtact tggggggctc gctccgcgcg agcgccacct cgcggcgggc cttgagcgcc
     1141 tccggatcgc agaagcagcg gtaggccttc tcttcgacca gcagcttctc cgccgcggcg
     1201 gcgtagagcg caagccgctg gctctggaag tagggtccga aatcgccccc cttgtccggc
     1261 ccctcgtccc aggtaagccc cagccaacgc atcccctcga agatggcgtc caccgcctcc
     1321 ttcgtggagc gctcccggtc ggtgtcctcg atgcggagga tgaagacgcc gccgtggcgg
     1381 cgcgcgaaga ggaagttgaa cagcgcggtg cgcgcgccgc cgacgtggag gtatcccgtc
     1441 ggactcggcg cgaagcgcac gcgaaccggc gcggtcattc ggtctcctgg ttggggtcga
     1501 atcgaaacgt tcgggacgga gcgccgcggg cggctgcgct cagcgcagca gcgccatctt
     1561 ccgcgtaaag gccgagccgc cgatctccgc gcggtagaaa tagacgcccg atgcgagcgg
     1621 cgcgccggaa tccccggctc cgtcccagcg ggcctcatga tagccgggat cgaaccggcg
     1681 cgaaaccagg gtgcgcacca gccgcccgtc cgccgcgtag atccgcaggg tcacgtccgc
     1741 cgccgcgggg cccggcaccc ggaagccgat cacggtgacg ggattgaagg ggttcgggcg
     1801 gttctgcgcc agggaggcgc gcgcctcggg ggatggagcc ggatcgggga ccggctgcgg
     1861 gtcggccgac gtgaggatcg ggaaggacgc gacgccggtg tgccagttgt cgtgatggaa
     1921 catcggccag gccatcccca ccgaagcgaa gctcccgggg tagcgccaga cgtgcaccgc
     1981 gccgtcccat ccggtgagcg cgatctcgca ggcgccgtcg ccgtccaggt cccacacggc
     2041 cggcgtggag cgcacctccg ctccgatctc gatcgggaac ccgctcaccg gcgtcccgtc
     2101 gaagtggaag gcgtagaggc ggcggtcctc cgccccgatc agcacttcga gcgctccgtc
     2161 gccgtttaag tcagcgacgg cgggcgacgc ctcgctgccg gcccccagct ggaccacgct
     2221 ccaccccggc aggatcgctc cggcaccgtc gtaggcgcgc acccgtccgt ccgcgccgca
     2281 gacgatcacg tcgagcgagg ggtcgccgag atgccggcgc agcggcgcca gcaccggcga
     2341 gggaacgcgc ccgttccccg gcgtcagctc caggggacgc ggccatcccg gcgcgcgctg
     2401 ccccgcggcg gtgatgaggt agaggctgtc gttcgcgcag gccatcgcgg tctccaggcc
     2461 gccgccggcg atgacgtcgc cgatcgcggg ggagctgctc atgagcccgt tggcgacgaa
     2521 cggccagccg gagaggggca cgccgtcgcg cagcgcgtag acgcggcccg ccgaggtgcc
     2581 gaagaccacc tcacggcggc cgtcggcatc caggtcggcg atcgccggcg aggagatcac
     2641 ggtgcccacg ggcacgaagt agaccccgtc ggtggcggga ttgccgtctc cgtcgcgaag
     2701 ctcggtcccg tccgcgcgcc aggcgtagag ccggctggtg ttggaaccga tcacgatgtc
     2761 gggcgcgctg tccccatcca gatcgcccac ggcgggcgac gaccagaacg gcgccgcgcc
     2821 cttcttgggc cacccgggaa ggagcgttcc attggcgcga aagaccgcca cgagcgagtc
     2881 gttccaggaa gcggcgatga tctccggcgt cccgtccagg ttgacgtcgg ccgcggcggg
     2941 agtcgaagcg atgttgtgga ggagcgtcga gaagacgccg agcgtgaccg ggttcgcgtc
     3001 gccgtcccgc cactccgatc cgtcggcatg gaacgcatag agctgatcgg cgcccgtgat
     3061 cacctccacg cgaccgtcgc cgtcgaggtc ggcgaggcat acggaggccg acgtcgcggc
     3121 ggccagcggc gccggccagc cggagacgga ggccggtgtc gtgctcacga ggagccaggg
     3181 ggttcgggcg ctccggtttc cgctcgagtc cacggcggcg accgcgaacc agtaggcggt
     3241 gagcggcgcc agccccgcgt tctcgaaggc ggaggtgcgc cgcaccggca gcggcgacgc
     3301 ctcgaccgga gccgcggtgg tgtccgctcc ggagcgcagc acgacgtagc cgagaagatc
     3361 cggcgcggcc accgcatccc aggagagcgt gacgccggcg acgcccgagg ccggggcgcg
     3421 aagccccgtc ggcgcgggag gcacaacgcg ttccacgggc agcgtccaga cgtggccgta
     3481 ggcgtccgtg atccggagat cgaacaggcg cgcgggcggc gcggtcgcgg cgtgaacgac
     3541 gatcgccgtg gtcgccgccg tccctccggg cgcgagggac ggcgcgctgc cgctcggagt
     3601 gacgaaggag atcccggagc cgggattgat ggcgagcagg gacggcgccc gtgcccggcc
     3661 gtccccttcg ttcccgagcg tccaggtgaa ggtggcgttc tcgccggcct ccagcactcc
     3721 gtttccgttc gtatcggtca tcgtggcttt cgtgaagagc agcgagggcg cggccacctc
     3781 gacgtccacg tcggccgtgt cggcccgggc ggcgtcgcgc gcgatgaagc gcaggcgcgc
     3841 gatgcccggc gcgttgggcg acgccagcgc gtgcgcgagc agcgagtcgg ggatcgtgag
     3901 ctgcgccccc gcggcgagcg cgccgaccac cgcggtggcc ttgtccacga ccaccggccc
     3961 gctcgccgcc ttgatctcga gcgtgaccgg cccgctgctc gccgcgccgc cggtgttctt
     4021 cacggcgccc gcgacgcgga aggactcccc cgcctcgatg agcccgtttc cgtcgccgac
     4081 gaccgcgtcg gtgggcgcca cgccgagcgc cgcgaaatgc gcgggcgcgt ccgcggtcac
     4141 gttcagcgag tccaggtagg ggcgcacccc gtccttcgtg acggcgagcg agaacctccc
     4201 cgtgaccgag ggatggaacg gaagcgtcgc ctgccccgcc gcgttggtga gcgccgtgac
     4261 gtagtcctcg cccgctttcc acgccacgac gagcgccgat tcggcgggag ttcccccggc
     4321 catcaccgtc acggcgatgc tccggcccga gagcggcatc gaggcgggcg cggtcacggc
     4381 gaacgaggaa ggagggcacc gccagatcgg aaggctgggc acgccgatca ggacgctctc
     4441 gaagtaggtc caccggtcgg gcgtctcggt cgaggcggcg ggaaggagcg ccgcgcgcgc
     4501 gtcctccatc gcctcgccga gcgtgatgcc cgaagcggga acgggatcgt ggaagaggcg
     4561 cgccgcgaga tcctccgtga acgccgccgc cggagagggc caggcgtccc gggtcgcgcc
     4621 gacgtaggcg aacgctcctc cgtcgggccg acgggtgagc cgctcggcga cgcagtcgta
     4681 gtccacgccg gccgaagcgc agttcgtcgc gatccagagc gccgccgaat cgccgttcgt
     4741 gatcgccgcg aggtccccga gcgtgaggag ctccgatccc acggagagct gcgaccgcgc
     4801 cccgtgaccg atctggaaca cggcgttgta gccgcgcgag agggcggaga gcgcgttggc
     4861 ccgattcagg tgcgcggccc cgggataggc cgtgtcgttc tcgtagtagc ggtccacgac
     4921 cgcgcagccc ggcgcgccgg ccgtcagcgc ctgtccctcc gcggcgccgt ccacctggac
     4981 gggcgcgccg ggggtccagt tctgcggcag gagcacctcg gccaggacga gctgcttcgc
     5041 gagcatcggg aggaacggcg cgccggcgta gcggagcgcc ttgtccacga gagccttcgc
     5101 gtcggcgcgg gtggccgcgg agagcctccc cacggcgatg tcgggccgca tgtcgaggcc
     5161 gtcgccggtc tcgccgtaga tcccgtttcc gttcgcgtcc caggtgccgt cgaggtcggc
     5221 gtagtaggcg tcggtgggga tgtcctccac ggtcgcgaag gtcacgcgga ccatccggag
     5281 cggcacggta tcgtggtccc ccgccaggat cgcccaccgg atcccccaga ggtcgcgcgc
     5341 ggatcgcaga aaggaacgga tcgcctgcgg caggtcgttc gagcgagggt cggccgcccg
     5401 gatcgtggag atcccccgaa cgacggtggg cctgccgatc cgcgtctggt agtcggccag
     5461 ccgctggaag atgtccgcca acgaatcggg ggtcacgatg acgcagtcga ccggcgagcc
     5521 ggatgtggac gggaggagcg tcggcgcaaa gggcgccgct ctgcccggct ggccgaagag
     5581 gcgcgtcgtc cggggcggca gggcaccctc ggccccaacc gcggtgaagg cgctcgccac
     5641 gatcaggagg aggagcagga ggagcgtgcg gcaattcgtg aaggaagaaa cggaaacggc
     5701 tgtcatcaac tcacttggac ggggtcttgg gatggaccgg ctcgcgcgcc gcttcccccg
     5761 atgctactcc tccgccatac gtggatgcaa tcggtcgtag atcgtccagt cgatcgcgta
     5821 gaccgaatag acgtaggggg tcggccgcgt gatccccagg tcggggatcg accgcgagaa
     5881 gaggaaccga tagggcgtcg ccggatcgtc gggaggggcc aggcgcccct ccctggtgct
     5941 ccgatcgatg aggtaggcgg gccggcccac cggctcgtac aacaggttcc agaccaccgc
     6001 gtcgtagccg tcgcgcacgg aggcccgggc catgccgggc gtgaccagtc cgtacaggtc
     6061 gagcaccggc cggtccgagt agtagccgaa ggcgccgata tcggggaccg cgaagagcgt
     6121 gcccggcgcg gtgtgcgccc tcgcccacag tccgagcgac gcgagcgagt cgcggagacc
     6181 ctgcgtgtgg cgccgcgcgt gcggcgcgct gaccgcgagg gtcacgtaga ggttctgcgc
     6241 cgcgaagagg aggagcgccg ccgcgagcgc ggccgcgcgc cgccggggcg cccagacgcg
     6301 agaggcggcg cgaagcgcgg ccagcccgat caggaggact gcgggcatcg cgggcaccag
     6361 gtagcgcgag accacctgca ccccttcggc cgcgaacgcg agcacgagga gcacgggcca
     6421 agccaggacc agtccccacc aggcgcgccg gccgcgcgcc ttgcccatcg cgctccaggg
     6481 gtgaagccac gccagggcga gcacggcgat cgccaccggc acgagatcgg ccgcgagcag
     6541 cgcgcggatc gactcgcgca gggccgaggc ggcccggccg ggatcgagcc atgccgccgc
     6601 cttggcggcg cccgtgttgg gcagcaggcg atggaaccac atccacgcca gggccagcca
     6661 gggcccggcc gtgagcgcgg ctcctcccgc gagggcggcg acccggccga cccgcgcgcg
     6721 gggctcggcg gcccatgcca cggcggccag cgagagcatg aacaggagca ggaactcggg
     6781 cctcgccagg aacgcggccc ccagcgcgag ccccgcccac gcgggactgc gcccatggag
     6841 caaggcgtgc gcgaggagca ggcacgcgag cagggcgagc gccagcgcga gcggggtttc
     6901 catcccggag atggcccatc gcaccgacca ggcgtccagc gcgaccatgg ccgatgccgc
     6961 gagcgcccat cgcggctccc agcggagcgc gcgcgcgatc gccgccatga ggagcaccga
     7021 ggccgccgtg aagagagttc cccagacctt cgcggcggcc gcgagcggcg gcatcgagtc
     7081 cgccgaggcg ggcgtcgcgc ccgcgccgtg gaggaacgcg cccgttccgg ccaggaggag
     7141 gacccagagc gggctggtgg cgccgtacgt gggctcgccc gcgttgaacg cgaaaccctc
     7201 gccccggatc aggtgcttcg cgaactggag atggatgaac gtgtcgtcgg taaggtagcc
     7261 gcggaacggg agaaggagcg ccagcgcgag gagagcgaga agtccgagcg ccgcgtgatg
     7321 gcgcgcccgc catggggagg cgggcccgtc gctcaccggg tcacgtccga tggacccacc
     7381 gggtcaagtc ctctcgagcc atcgcgccac ccgcgccgcc accgcgtcgg gggtgaaacc
     7441 cagctccttc gcgatcactt cggcgggcgc cgaggcgccg tagcgcgaga ggccgatccg
     7501 gagcgcgtcc tttcccacga tcgccgccca cggttccgtc cgtgcggcct cgatcaccac
     7561 cgtcgccacc ccgtcgggaa
//
LOCUS       DASSLZ010000217         4822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_837551, whole genome shotgun sequence.
ACCESSION   DASSLZ010000217 DASSLZ010000000
VERSION     DASSLZ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837551"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VFM00_08340"
     CDS             complement(<1..1050)
                     /locus_tag="VFM00_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498294.1"
                     /translation="MWLPREAVELEAPDRLEERDPGPGMGARADVRNGRWKPKHVWAA
                     TAWRPLGSMRAEWVALAEGAGWGTLRIQRQPRVALVAGAIVVRRPPSLLGEALGLTRP
                     LRAPRGPEGGVPRMEGPRGATSLAVRGAASEATVGPVATWGLVGRDGDDRVLGAGGLA
                     SRRGALTAAIAAGAIRGGRRSGSLSVGWTAGEDRFAGEALLSPGRGPELLAEAVRSAG
                     PLAVEARWRRRPGEARPVAGELVARWGLGPMRGRLCWRPWSARAPGDDGRVELETTVR
                     DAGRGPIRMRLGARAAEDALPYSGGSRGERYAIADIGIARERGRAFSLLVSVRDSRRA
                     AGGAIASAIGGRLALS"
     gene            1436..4246
                     /locus_tag="VFM00_08345"
     CDS             1436..4246
                     /locus_tag="VFM00_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7498295.1"
                     /translation="MRPLRSRIVFLTLVLAAAIPATAHAARTAQAGGEPPPAGLLRNQ
                     ASSVEMLPAGMKSARIQRAPAAGETVYVFRDSLETRSSPSNEGGWTHYDQSLKPTAWH
                     VDSLLSCQGAAWWCGIVDSTWIYDTNRAGYDNDWTQYLSNIVDLSGIPAGTPVTLGFR
                     HRFNAEPLYDFGSVEVFDPDDGDWNKLASFTGAVPNNQNNCDTVTVVIPDSTRLKYNP
                     VSFRFKFTSDVGYSSADGLYNGDGWILDNITIKAGTDVRLFDDGHGNAGHWSVTTNPG
                     VGDLFWLSGNLFTEDVCSSNTSKVWTAWDPVVQSLVPRMDDLLNLPPVAINRASDAFV
                     EFDVYRNLPLQACFYYHLNFRTKNVGGNWSAWQDPTFFVYYGGNKDWARQRIPLPGAG
                     EHDSLQVQLGLRDYSATFCDGASSPNGVYAFFDNVAIGVIGTAPPSFVVRDIDLFNDT
                     FQTTPFYKDDNFNSAVGDTTVVNVNTSLGYKNGFMFYRTASSGAFSSTPLVNVAPAIP
                     TRFYADVPAASYPAGTTLQYYFAATDSLNNTSTYPTDAVSAQHYLTASILPLKTATNP
                     ANGCFDSLATILFVNHFSGRETTPRIADALKALGYKFDTWDVNGPSSGVGNCLGGSDP
                     NDIQYHWPPTDVASLTQYKTIIWHSGDLSSFTITPQDEAVLQSWIQQSGKDRNLWITG
                     DDVGYELTASGADYNSFMAFTTGARYLRDSWETQPADTLHPAVSGVAGGPAAGRLFHL
                     NGDCPIINKFDLITPSTQAQAGGKWGILLRYPNTQPAATRYATKYVTFGADSARAVFM
                     GFSFNSIEEGGERLQLAKAITQNYFLENPCYTPTAVLEDPASQAPPARTQLFQNAPNP
                     FNPATTIRFSVAQPGPVRIDIFDVGGRHVRTLVEKSYEPGVYTARWDGVDDAGRPLAS
                     GAYFYRMTAPGASDAKKLILLR"
     gene            4622..>4822
                     /locus_tag="VFM00_08350"
     CDS             4622..>4822
                     /locus_tag="VFM00_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498296.1"
                     /translation="MDDPVGVENDVHAPISAHEPRGDPRASGPPRGFRIAGEPERDPL
                     RARAEPDRARAALFEREGLDRLG"
BASE COUNT          772 a         1844 c         1524 g          682 t
ORIGIN      
        1 ggagagtgcc aggcgccccc cgatcgcgga cgcgatcgcg ccccccgccg cccggcggga
       61 atcccgcacc gagacgagga gcgagaacgc gcgcccgcgc tcgcgcgcga tcccgatgtc
      121 ggcgatcgcg tagcgctcgc cccggcttcc gccggagtag gggagcgcgt cttcggcggc
      181 acgcgccccc agccgcatcc ggatcggacc tcggccggcg tcccgtaccg tcgtctccag
      241 ctcgacgcgc ccatcgtcgc ccggcgccct cgcggaccag ggacgccagc acagccgtcc
      301 ccgcatcggg cccaggcccc agcgcgccac gagctctccg gcgaccggcc tcgcttcacc
      361 cggcctgcgg cgccaacgcg cctcgacggc aagcggcccg gcggagcgga ccgcctcggc
      421 caggagctcc gggccccgcc cgggcgagag cagcgcttcg ccggcgaagc gatcctcgcc
      481 ggcggtccag ccgaccgaca gggaaccgga gcgcctcccg ccgcgaatcg cgcccgcggc
      541 gatcgccgcg gtcaaggctc ctcgtcgcga cgcaagtccc ccggctccaa ggacgcgatc
      601 gtcgccgtcc cttccgacca gcccccaggt cgcgacgggg cccaccgtcg cctccgacgc
      661 ggcgccgcgc acggcgaggc tcgtcgctcc acggggcccc tccatccgcg gcacgccgcc
      721 ctcgggtcct cgcggggcgc ggagcggacg cgtgagcccg agcgcttcac ccaagagcga
      781 cggcggacga cgcaccacga tggcgcccgc gaccaaggcc acgcgcggct ggcgttgaat
      841 ccggagcgtt ccccagcccg cgccttccgc cagcgcgacc cactccgctc gcatcgagcc
      901 cagcggccgc cacgccgtcg ccgcccagac gtgcttcggc ttccagcgac cgtttcgaac
      961 gtcggcccgc gctcccatgc ccggaccggg atccctctct tcgagacggt cgggcgcctc
     1021 gagttcgacg gcctcacggg ggagccacac tccggcggac tcggcgtctt gcgccgccga
     1081 tgcgcgcggc gccgcgacga gggccgcgat gagtcccgaa atcgctagtt gccttttccg
     1141 tcgcatgata gcaaagcgga agagggccaa caacgcgacc ggaccggacc atggaacgca
     1201 gagcgtctgg aggccgcaag ctgaacgcgc gacacgattt ccctcgactt cggcggcgcg
     1261 atcggttatc atgccgcctt cagcgtagcc acgctcggtt tcccgtcgct tgcctctgtc
     1321 gcgacacctc ggactccaag ggaacacgaa ggcgacatgg cacgttttcc catccccaat
     1381 caccgtttcc ccccgtttcc aggccgcgcc ccgtatcgtc cggaggactc cccccatgcg
     1441 tccgcttcgt agccgcattg tcttcctcac cctcgttctc gccgccgcga ttccggcgac
     1501 agctcacgcg gcccgcacgg cgcaggccgg aggggagccg cccccggcgg gactcctgag
     1561 aaaccaggcc tcgtccgtgg agatgctgcc cgcggggatg aagtccgcgc gcatccagcg
     1621 cgcgcccgcg gccggagaaa cggtctatgt gtttcgcgac agcctggaga cgcgctccag
     1681 tcccagcaac gaggggggtt ggacgcacta cgaccaatcg ctcaagccga cggcgtggca
     1741 cgtggacagc ctgctgagct gccagggagc ggcctggtgg tgcgggatcg tcgattcgac
     1801 ctggatctac gacacgaatc gggcgggcta cgacaacgac tggacgcagt acctgtccaa
     1861 tatcgtcgac ctgagcggga tccccgccgg cacgccggtg acgctcggat tccggcaccg
     1921 gttcaacgcc gagccgttgt acgacttcgg cagcgtcgag gtcttcgacc cggatgacgg
     1981 cgactggaac aagctcgcga gcttcacggg tgccgtcccg aacaaccaga acaactgcga
     2041 cacggtgacg gtcgtcatcc ccgactcgac gcggctcaag tacaacccgg tctcgttccg
     2101 gttcaagttc acgtcggacg tcgggtactc ctcggccgac gggctctaca acggcgacgg
     2161 ctggatcctc gacaacatca cgatcaaggc gggcaccgac gtccggctgt tcgacgacgg
     2221 ccatggcaac gcgggccact ggtcggtgac gacgaatccc ggcgtgggcg acctgttctg
     2281 gttgagcggc aacctcttca ccgaggacgt atgctccagc aacacctcga aggtctggac
     2341 cgcgtgggat cccgtcgtcc agtccctggt cccgcgcatg gacgacctcc tgaacctccc
     2401 tcccgtcgcg atcaaccggg cgtcggacgc cttcgtagaa ttcgacgtct accggaacct
     2461 gcccctccag gcgtgcttct actaccacct gaacttccgc accaagaacg tgggcgggaa
     2521 ctggagcgcc tggcaggatc cgaccttctt cgtctactac ggcggcaaca aggactgggc
     2581 gcgtcagcgg attccgctcc ccggcgccgg cgagcacgat tcgctgcagg tccagctggg
     2641 tctgcgcgac tactcggcca ccttctgcga cggcgcctcg tccccgaacg gcgtctacgc
     2701 gttcttcgac aacgtcgcca tcggcgtgat cggcaccgct cctccctcgt tcgtggtgcg
     2761 tgacatcgat ctcttcaacg acaccttcca gaccacgccg ttctacaagg acgacaactt
     2821 caacagcgcg gtcggcgaca cgacggtggt caacgtgaac acgtcgctcg gatacaagaa
     2881 cggtttcatg ttctaccgga cggcttcgag cggcgccttc agctccacgc cgctcgtcaa
     2941 cgtcgccccg gcgatcccca cccgcttcta tgccgacgtg cccgcggcca gctatccggc
     3001 cggaaccacg ctgcagtact acttcgccgc cacggacagc ctgaacaaca cgtcgacgta
     3061 cccgaccgat gccgtctcgg cgcagcacta cctgaccgcg tcgatcctgc cgctcaagac
     3121 ggccaccaac ccggcgaacg ggtgcttcga cagcctggcc acgatcctgt tcgtgaacca
     3181 cttctccgga cgcgagacga cgccccggat cgccgacgcg ctgaaggcgc tggggtacaa
     3241 gttcgacacc tgggacgtga acggacccag cagcggcgtg gggaactgcc tgggaggctc
     3301 cgaccccaac gacatccagt accactggcc cccgaccgac gtcgcctcgc tgacgcagta
     3361 caagacgatc atctggcact cgggcgatct ctcgtccttc acgatcacgc cgcaggacga
     3421 ggccgtattg cagtcgtgga tccagcaatc cggaaaggac cggaatctct ggatcaccgg
     3481 cgacgacgtg ggctacgagc tgacggccag cggcgccgat tacaacagct tcatggcatt
     3541 caccaccgga gcccgttacc tgcgcgacag ctgggagacg cagccggcgg atacgctcca
     3601 tccggcggtc tcgggggtgg cgggcggtcc ggccgccggg cggctcttcc acctgaacgg
     3661 cgactgcccg atcatcaaca agttcgatct catcaccccg tccacgcagg cgcaggcggg
     3721 cggcaagtgg ggcatcctgc tccgctatcc gaacacgcag cccgccgcga cccgctacgc
     3781 cacgaagtac gtcacgttcg gcgccgacag cgctcgcgcc gtcttcatgg ggttcagctt
     3841 caactcgatc gaggagggcg gcgagcggct gcagctggcc aaggcgatca cgcagaacta
     3901 tttcctggag aatccctgct acacgccgac ggcggtgctc gaggatcccg cgagtcaggc
     3961 cccgccggcc cgcacgcagc tcttccagaa cgcgcccaat cccttcaacc cggccaccac
     4021 gatccgcttc tcggtggcgc agccgggtcc cgtccggatc gacatcttcg acgtcggcgg
     4081 gcgccacgtg cgcacgctcg tcgagaagtc ctacgagccg ggcgtctaca ccgcgcgctg
     4141 ggacggcgtg gacgacgccg gacggccgct cgcgagcggc gcctacttct atcgcatgac
     4201 ggccccgggc gcgtccgacg cgaagaagct catcctgctc cgctgacctc gccgcgcgat
     4261 cgcgcgcgag gcgctcctcc gggggacggt ccgctctcga cgggggccgt cccctttcgt
     4321 cacgggcgag cccgatgctc atggcggcga tgccgccgcc ggccgcgccg tccaggcgtc
     4381 ccgccgcgat cgcgacgcgc ccccatcgcg ccgcgagcga gaacccgatc cgtcccggac
     4441 cctcgccgcg cgcggcacga agggtgagaa gcgacgcgac cggcagctcg atcgcgagcc
     4501 gggggctcct ccggcctgtc cgttcccagc ggtcggccag cgccaccgtc accccgcccg
     4561 attggaatcg cacggccgcc gccacggagg ggtcggcacc gaggcgctcg gcgccgcggc
     4621 tttggacgat ccggtcggcg tcgagaacga cgtccacgcc cccatatccg cccacgagcc
     4681 gcgcggcgat ccccgcgcct ccggtccacc ccgcggcttc cggatcgccg gcgaaccgga
     4741 gcgcgacccg ctccgcgcgc gcgcggagcc ggaccgcgcg cgtgccgcgc tcttcgagcg
     4801 cgagggattg gaccgactcg ga
//
LOCUS       DASSLZ010000218         6390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_966726, whole genome shotgun sequence.
ACCESSION   DASSLZ010000218 DASSLZ010000000
VERSION     DASSLZ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966726"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VFM00_08355"
     CDS             complement(<1..672)
                     /locus_tag="VFM00_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HET7498297.1"
                     /translation="MTFVVRPTVLPPAVWVPLFAILLSAPAHAQSPVPIATHVGTEQS
                     ERLGLTTAIVGDLDRDGCADYLIGAPGSSPGGVSFAGSAFLYSGASGAVLRQIDGTQA
                     AEQRGFVAALGDLDGDSIPDYAVAAPLVPNGATPLAGSIWVVSGKTHQTLYDLRGPTE
                     RAKFGTVMTGIGDADGDGLADMAIGSWPIDLNGLVEVGVVYVVRGVDGALIRSFNGQF
                     EGQRFG"
     gene            complement(738..2171)
                     /locus_tag="VFM00_08360"
     CDS             complement(738..2171)
                     /locus_tag="VFM00_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HET7498298.1"
                     /translation="MATGLGKRIRALRAKEGLSQAAMAAKLGISASYLNLVEHDRRPL
                     SANLLLALAQRFEVDLRAFSGGEDSQISADLLEAFGDPLFESQTVSERDVREFVATTP
                     DLARAVLHLHHAYTAARGSAEMLAAEVVDRQDLAGIDRSGPSSEQVSDLIQRHQNHFP
                     ELEAEAERLWKDGKLDTEDLFGALAEYLAKRLKVRVKVLTVSEMGGAVRRFDPERREL
                     WISEVLRRGSRNFQLAHQVGLLTCSDTLDLMSRDPSLTSDEARSLCRVALANYFAAAV
                     LTPYEEFQRAAESLRYDLDLLGHRFRVSYEQVCHRLTTLARKGAKGVAFDMVRIDLAG
                     NISKKFSATGIRLPRFGGLCPLWNVHTAFLQPGVIHTQLSRLPDGNAFFSVARTVRKH
                     RGGYRAPDVLYSLQLSCDVDSARKLIYADGYDLVNLAASTPVGITCRLCERIDCRARA
                     FPSVHERLRVDENVRGVSFYAPVKEEE"
     gene            2364..3980
                     /gene="aceB"
                     /locus_tag="VFM00_08365"
     CDS             2364..3980
                     /gene="aceB"
                     /locus_tag="VFM00_08365"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HET7498299.1"
                     /translation="MEATTITRERAAGIEVRGPVRPLYDEILTAEALSFLARLTREFR
                     PRIDEALARRKERQARLDAGEKLEFMNLHPEDWTVAPIPADLMKRAVEITGPVDRKMV
                     INALNSGADCFMADFEDSNAPTWDNNVQGQLNLRDAVRRKIAFSDPASGKSYALNERI
                     ATLLVRPRGLHLPERHLLVDGTPMPGSLFDFGLYFFHNARELMARGTGPYFYLPKMES
                     HREAAIWNDVFLASQDALGVPRGSIRATVLIETLPAAFEMDEILWELREHSGGLNCGR
                     WDYIFSFIKTRAHEAGAVLPDRSQVTMDQPFLRAYTQLAVKTCHRRGIHAMGGMAAQI
                     PIKSDPEANEKALAKVRADKLREVKDGHDGTWVAHPGLVPIAREVFEAHMKGPNQIDR
                     KRDDVEASAPALLQVPEGTRTEEGLRLNARVGIQYLASWLCGNGCVPLYNLMEDAATA
                     EISRTQVWQWIRHGATLEDGRPVTPDLFRRVVDDEMTRIAGDVGPERFRDGRFDEARA
                     LFVRLSTAEALEPFLTLPAYDLLDDRVDRD"
     gene            4013..5353
                     /gene="aceA"
                     /locus_tag="VFM00_08370"
     CDS             4013..5353
                     /gene="aceA"
                     /locus_tag="VFM00_08370"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HET7498300.1"
                     /translation="MKSRIRQTGPFEKRDRWAGIVRPYSDRDVAALRGTVQVEHTLAR
                     RGAERLWALLHDEPHVSALGALTGNQAVQQVKAGLQAIYLSGWQVAADANLAGQTYPD
                     QSLYPANSVPSVVRRINQALLRADQIENAERIRAAEKNGHANGNGNGNGHGPAKAPRF
                     WLAPIVADAEAGFGGPLNAFELMKGMIEAGAAGVHFEDQLSSEKKCGHLGGKVLIPTG
                     HFIRTLVAARLAADVMDVPTLVVARTDADSARLVTSDIDPRDHEFLTGERTPEGFFRM
                     RGGLDCAIARAKAYAPYADLLWCETSTPDLDEARTFAEGVHREFPGKLLAYNCSPSFN
                     WKKNLDEGTIAKFQRELGAMGYKFQFVTLAGFHALNHSMFELARAYKEKGMTGYVELQ
                     EAEFASEKDGYSATRHQREVGTGYFDEVMQVIAAGSDCTTLALKESTEAAQFKA"
     gene            5388..5636
                     /locus_tag="VFM00_08375"
     CDS             5388..5636
                     /locus_tag="VFM00_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498301.1"
                     /translation="MRHPSARGVGVRYLIAVMLPISGAVLVVYTAVQLAIAHVTHNVP
                     PPRRIAEATIPADDLPALTGHRSEPLPAHEVVPAPGTR"
     gene            5670..6359
                     /locus_tag="VFM00_08380"
     CDS             5670..6359
                     /locus_tag="VFM00_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498302.1"
                     /translation="MFRRIHVVVRLAAAALAAACLAASPVPPPPGRTETGFDTVPGAV
                     RALLHPTTALLPAFPNESPAALDPSRWAPSVRDSDRVCVLELRTYVRGTPVTLSDIRQ
                     RKAGQSLSARYLCGSRTWRGPRYAGPFYVWDGQNALIERSYRVTDGARYREDLYQYRG
                     NGLVWAYRHRERNEDQSGPFFMLDEYYDPDGRLAGFSVERTAPDSLALRWRRGAVVEE
                     EAFRKWTGEFR"
BASE COUNT         1055 a         2186 c         2201 g          948 t
ORIGIN      
        1 tccgaagcgc tggccctcga actggccgtt gaagctgcgg atcaaggctc cgtcgacccc
       61 tcgcaccacg tagaccacgc ccacttcgac gagcccgttc aagtcgatcg gccaggaccc
      121 gatcgccata tcggcgaggc cgtctccgtc ggcatccccg atgcccgtca tcaccgtgcc
      181 gaacttggcg cgctcggtcg ggccgcggag gtcgtagagg gtctggtgcg tctttcccga
      241 gacgacccag atggaccccg cgagtggagt cgccccgttc ggtacgagcg gtgccgcgac
      301 cgcgtagtcg gggatcgagt cgccgtccaa gtcacccagg gccgcgacga atccgcgctg
      361 ctccgcggcc tgggtgccat cgatttggcg gagtacggct cccgaagcgc cggaatagag
      421 gaaggccgag cccgcgaacg acacgccgcc ggggctcgac ccgggcgccc cgatcaggta
      481 gtcggcgcag ccatcccggt cgaggtcgcc cacgatggct gtcgtgaggc cgagccgctc
      541 gctctgttcc gtaccgacgt gggtggcgat gggcaccggc gactgagcgt gcgcgggtgc
      601 ggaaagaagg atggcgaaga gtggaaccca gacggccggg gggaggaccg tggggcggac
      661 gacaaaggtc atgacgcgcc tcccggttag ggccgcagca tagcaccggc accggcgcgg
      721 tccagttctc ccttggtcta ctcctcttcc ttcaccggcg cgtagaagga caccccccgc
      781 acgttctcgt ccacccggag ccgctcgtgc acggacggga aggcgcgcgc gcggcagtcg
      841 atgcgctcgc agagccggca ggtgatcccg accggcgtgg aggcggccag gttcacgaga
      901 tcgtagccgt cggcgtagat cagcttccgg gccgagtcca cgtcgcagga gagctgcagg
      961 gagtagagca cgtcgggggc gcggtagccg ccgcggtgct tgcgcacggt gcgcgccacg
     1021 gagaagaagg cgttgccatc gggcagccgg gatagctgcg tgtggatgac gccgggctgc
     1081 aggaaggcgg tgtgcacgtt ccagagcggg cagagcccgc cgaagcgcgg aagccggatg
     1141 ccggtcgcgc tgaacttctt cgagatgttt cccgccaggt cgatccgcac catgtcgaag
     1201 gcgaccccct tcgccccctt ccgggccagc gtggtgagcc ggtggcagac ctgctcgtat
     1261 gagacgcgga agcggtgccc caggagatcc aggtcgtagc gcaggctctc ggcggccctc
     1321 tggaactcct cgtagggggt gagcacggcc gcggcgaagt agttggccag cgccacgcgg
     1381 cagagcgagc gcgcctcgtc ggacgtgagc gagggatcgc gcgacatgag atcgagcgtg
     1441 tcggagcagg tgaggagccc gacctggtgg gcgagctgga agttgcggga gccgcgtcga
     1501 agcacctcgg agatccagag ctcgcgccgc tcggggtcga agcgccgcac cgcgcctccc
     1561 atctcgctca cggtcagcac cttcacgcgc accttgagcc gcttggccag gtactcggcc
     1621 agggcgccga agagatcctc ggtgtccagc ttcccatctt tccagagccg ctcggcctcg
     1681 gcctccagct cgggaaagtg attctggtgg cgctggatca gatcggagac ctgctccgag
     1741 gaggggccgc tccggtcgat cccagcgaga tcctggcgat ccacgacctc ggccgccagc
     1801 atctccgccg agccgcgcgc cgcggtgtag gcgtggtgca ggtgcagcac ggcgcgcgcg
     1861 agatcgggcg tcgtggccac gaactcgcgc acgtcgcgct ccgagacggt ctgcgattcg
     1921 aagaggggat cgccgaaggc ctcgaggaga tcggccgaga tctgggagtc ctcgcctccg
     1981 gagaaggcgc ggaggtccac ctcgaagcgc tgcgccaagg cgaggaggag attggcgctc
     2041 agggggcgcc ggtcgtgctc gaccaggttc aggtaggagg cggagatccc cagcttggcg
     2101 gccatcgccg cctgcgagag gccctccttg gcgcggagcg cccgaatccg cttgcccagc
     2161 ccggtcgcca ttcatgaagt ttacatgatt tacaacttta cagtaaagag cacactttca
     2221 ttatcacgtt aactttaaat atatcaaata cttgttgact tcggactcgg gtacctttac
     2281 tatcgtgaat gtcgtcaacg aggggtggga cgagctgacg gcgcgtcctg cccctttgct
     2341 gttcgaagta caggaggtga accgtggaag ccacgacgat tacccgcgag cgcgcggccg
     2401 gcatcgaggt acgcggcccc gtgcgcccgc tttacgacga gatcctgacg gccgaggcgc
     2461 tgtccttcct cgcccgcctg actcgggagt tccgcccccg catcgatgag gcccttgccc
     2521 gccgcaagga gcggcaggcg cggctggacg cgggagagaa gctcgagttc atgaacctgc
     2581 accccgaaga ttggacggtc gcgccgatcc cggccgacct catgaagcgc gcggtcgaga
     2641 tcaccggacc ggtcgaccga aagatggtga tcaacgccct gaactcgggc gccgactgct
     2701 tcatggcgga cttcgaggat tccaatgcgc cgacctggga caacaacgtc caggggcagt
     2761 tgaaccttcg cgacgccgtg cgccggaaga tcgccttcag cgatccggcg agcggaaagt
     2821 cctacgcgct gaacgagcgc atcgcgacgc tcctggtccg tccccgcggg ctccatcttc
     2881 ccgagcggca tctcctcgtg gacggtaccc ccatgccggg ctccctgttc gatttcggcc
     2941 tctacttctt ccacaacgcc cgcgagctca tggcgcgcgg caccggcccc tacttctacc
     3001 tccccaagat ggagtcgcac cgcgaggcgg cgatctggaa cgacgtgttc ctggcgtccc
     3061 aggatgcgct gggggtgccg cgcggatcga tccgcgcgac ggtgctgatc gagaccctgc
     3121 ccgccgcttt cgagatggac gagatcctct gggagctgcg cgagcactcg ggcggactca
     3181 actgcggccg ctgggactac atcttctcct tcatcaagac gcgcgcgcac gaggccggcg
     3241 cggtccttcc cgaccgctcg caggtcacga tggatcagcc gttcctgcgc gcctacacgc
     3301 agctcgccgt gaagacctgc caccgccgcg gcatccacgc gatggggggg atggcggcgc
     3361 agatcccgat caagagcgac cccgaggcga acgagaaggc gctggccaag gtgcgcgccg
     3421 acaagctgcg cgaggtgaag gacggccacg acggaacctg ggtcgcccat ccgggcctgg
     3481 tcccgatcgc gcgcgaggtg ttcgaggcgc acatgaaggg accgaaccag atcgaccgca
     3541 agcgcgacga cgtggaagcc tccgcgccgg cgctgctcca agtgccggaa ggcacgcgca
     3601 ccgaagaggg gctccgcctg aacgcgcggg tcggcatcca gtacctggcg tcgtggctct
     3661 gcggcaacgg atgcgtgccg ctctacaacc tgatggaaga cgcggcgacc gcggagatct
     3721 cgcggacgca ggtctggcag tggatccggc acggcgcgac gctcgaggat gggcggcccg
     3781 tgaccccgga tctcttccgg cgcgtcgtgg acgatgagat gacgcggatc gccggcgatg
     3841 tggggccgga gcggttccgc gatggacgtt tcgacgaagc gcgggcgctg ttcgtgcggc
     3901 ttagcacggc cgaggcgctc gagcccttct tgacccttcc cgcctacgat ctgctggacg
     3961 atcgggtgga tcgggactga ccgcgcgacc tgcgctacgg gagaacacac caatgaaatc
     4021 gcgaattcgt cagaccgggc cgttcgagaa gcgggatcgc tgggcgggga tcgtccgccc
     4081 ctactccgat cgggacgtcg ccgcgctgcg cggcacggtc caggtcgagc acacgctcgc
     4141 ccgccgcggc gccgagaggc tctgggcgtt gctgcacgac gagccgcacg tgtcggcgct
     4201 gggcgcgctc accggcaacc aggccgtgca gcaggtcaag gcggggctcc aggcgatcta
     4261 cctgagcggc tggcaggtgg ccgccgacgc caatctggcg gggcagacct accccgacca
     4321 gagcctctat cccgcgaaca gcgttcccag cgtggtgcgc cggatcaacc aggcgctcct
     4381 gcgcgccgac cagatcgaga acgccgagcg gatccgcgcc gccgagaaga acggccacgc
     4441 gaacggcaac ggcaatggaa acgggcacgg acccgccaag gcgccgcgct tctggctggc
     4501 tccgatcgtg gccgacgccg aggccggctt cggcggcccg ctgaacgcct tcgagctgat
     4561 gaagggcatg atcgaagcgg gcgccgcggg cgtccatttc gaggatcagc tctcgagcga
     4621 gaagaagtgc ggacacctgg gagggaaggt gctcatcccc accgggcatt tcatccgcac
     4681 cctggtcgcg gcgcgactgg ccgccgacgt catggacgtc cccaccctgg tggtggcgcg
     4741 caccgacgcc gacagcgccc gtctcgtgac aagcgacatc gacccgcgcg accacgagtt
     4801 cctgaccggc gagcgcaccc ccgagggatt cttccggatg cggggcggcc tggactgcgc
     4861 catcgcgcgg gcgaaggcct acgcgcccta cgccgacctc ctctggtgcg agacctcgac
     4921 gcccgacctg gacgaggccc gcacgttcgc cgagggcgtg caccgggagt tcccggggaa
     4981 gctcctggcc tacaactgct cgccctcgtt caactggaag aagaacctgg acgaggggac
     5041 catcgccaag ttccagcgcg agctgggcgc gatgggctac aagttccagt tcgtcaccct
     5101 ggccggcttc cacgcgctca accactcgat gttcgagctg gcgcgggcct acaaggagaa
     5161 gggcatgacg ggctacgtgg agctgcagga ggccgagttc gcctcggaga aggacggcta
     5221 ctccgccacg cgccaccagc gcgaggtggg caccggctat ttcgacgagg tgatgcaggt
     5281 gatcgcggcc ggcagcgact gcacgaccct ggcgctcaag gaatccacgg aagcggccca
     5341 gttcaaggcc tgatcaagat tgggaggcgc atgccgatga ttccggcatg cgccatccct
     5401 ccgcccgcgg cgtcggcgtt cgctacctga tcgccgtcat gctgcccatc tccggggccg
     5461 tcctcgtcgt ctacaccgcg gtgcagcttg cgatcgccca cgtgacgcac aacgtgccgc
     5521 cgccgcggcg catcgccgag gccacgatcc ccgccgacga ccttcccgcg ctgacggggc
     5581 atcgttccga gccccttccc gcgcacgagg tcgtccccgc gcccggcacg cgctagaatc
     5641 acctcccgga caccctggga gggagcacct tgttccgccg aatccacgtc gtcgttcgcc
     5701 tggccgccgc ggccctggcc gccgcatgcc tcgccgcctc gcccgtaccg ccgccgccgg
     5761 ggcgcaccga gacggggttc gacaccgtgc ccggagccgt tcgcgcgctc ctgcacccca
     5821 cgaccgccct cctcccggcc tttccgaatg agagccccgc ggccctcgat cccagccgct
     5881 gggctcccag cgtccgcgac agcgaccggg tctgcgtcct ggagctgcgc acctacgtgc
     5941 gcggcactcc ggtaacgttg tcggacatcc ggcagcggaa ggccggacag tccctctcgg
     6001 cgcgctatct gtgcggctcc cgcacctggc gcggaccccg ctatgccggc cccttctacg
     6061 tctgggacgg ccagaatgcc ttgatcgaac ggagctaccg cgtgacggac ggggctcgct
     6121 accgcgagga tctctatcag taccggggga acgggctcgt gtgggcctat cgccaccgcg
     6181 agcgcaacga ggaccagtcg ggtcccttct tcatgctgga cgagtactac gacccggacg
     6241 ggaggctggc gggattctcg gtcgagcgga cggcgcccga ctcgctggcg ctccgctggc
     6301 ggcgcggggc cgtggtcgag gaagaggcgt tccggaagtg gacgggggag ttccgctagg
     6361 aaggaaacgg ggccgtcccg ccaggtgaag
//
LOCUS       DASSLZ010000219         5388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_837978, whole genome shotgun sequence.
ACCESSION   DASSLZ010000219 DASSLZ010000000
VERSION     DASSLZ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837978"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFM00_08385"
     CDS             complement(<1..668)
                     /locus_tag="VFM00_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7498303.1"
                     /translation="MRLPRFMRPVWSLAALGLALTLLTAPVAARADDDDDDNGRQGTV
                     KIYGGDDAQGGYLGVQVQDVTRALQRARGLTTDEGALVSRVEDESPADQAGIRRGDVI
                     TEVDREKIDSSQDLIKVVRGLSPGSRARVTLWRSGSLRTVTVQLGERPAGMMPGMGMP
                     DIRFRTGDDDHPMPPPSQMNDRDVQQQIRDLRDQLRELRQEIQSLRREMQRSNGRDDD
                     HDNDR"
     gene            complement(772..2118)
                     /locus_tag="VFM00_08390"
     CDS             complement(772..2118)
                     /locus_tag="VFM00_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET7498304.1"
                     /translation="MVPRARPTNPVRRHGGAANTAALAALILLALVLTAAAPRGARTK
                     RRAVPRPPRALQALQARIDAAARGDTIRVAPGVYPGPFRITKPLVLLAEGGAEKTILD
                     GRDSVRVLHIEGTNAVSVLGFTIRRGHSNSGGGIYCLRDSTVQIVDCVFTGNWESAVS
                     IWDSQGIVIGGCTLRENKGSAIDANNSVVAIYESKFYDNEGHEGGAISLSRTRLLVPL
                     RNLVFERNRAVGSVGGAVCAEDSSQGTVVNCSFKENSSDVAGGAIGISGGSAFNISRS
                     TFEKNQAGAGGAVQCDHSRMNMGLCLFNQNSSLAFGAAMGIGGRGMSNINPTLQSNTF
                     FENHVKGDGATLFFTDVSPEVRKNIFVVDHGQRAVTGLQTSPRYDCNLIWDPSGGAIG
                     ALPSVNTWVGDPLFCDAEHGNFALRDLSPALRAPCGPIGAFIGKAGCSSFRLQPAN"
     gene            complement(2108..4846)
                     /locus_tag="VFM00_08395"
     CDS             complement(2108..4846)
                     /locus_tag="VFM00_08395"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7498305.1"
                     /translation="MTAPAAKSPVLETTLDNGLTVLIQEVHTAPVAAFMVWYKVGSRN
                     ESAGITGISHLLEHMMFKGTPTYGKGEISRLLQKNGATFNAGTSLDYTTYFEVLASDR
                     LEMAMKIEADRMVHATIPDDEHRLEMTVVRSELERNEDNPHRALYLEAMAQAFKAHPY
                     HWPTIGWRTDVEQIRTSQIRDYYASHYMPNNATVVVVGDVNTQAALAMVKRYFGAVPK
                     GTEPPPVTTVEPPQMGERRFKIRKPGDTRYLLAAWRNPAVTHPDTYALDVLGIILGHG
                     QTSRLYQALVEKKLAAEVDASNDNGRDPLLFNAQATAAPGVGLDRLEAALLDEVERLK
                     SEPVTAKELERAKKQVEASFIYSKDSIRSLAQQLGYYNTVASWRYLDTYVERVRAVTT
                     ADLQRVARTYLTENSRTVGWYDPTPEGQADAGDGAPAEAAGAATPSRWVDHYRPGDAA
                     DALPGLPASVSEAMPDERAMNAAAPGAAAAAPAGVMHGAAPDRTVLPNGLTLIVRNNP
                     ANPTISLQGLVKAGGIYDPPERSGLAGFTAAMLDQGTAKRTALQTAETIEGAGAHLSF
                     DGGSETVSISATMLSADLPTVLDVLADALQHPAFPADKMEKIRAERITDYQVAENSTA
                     SVAARRANGLLYPKGHPYHVNPMGTDTTLRAITREDLAAFHRSHYGPNNTILVLVGDV
                     DTKRAVELVRAAFGGWAKLEPAPPFEVPKAQAPATTLELRVPMKGKSQTDVVYAVPGI
                     PRTAPDYDAAMVMNYILGGGSLSSRLMNSLRDEQGLVYGVYSSFAAGIGAGPLSIRAG
                     TNPANADRAVASILEQVRRMAEQGPTDAEMEDAKDYLTGVFPVRLETNAGVASQLVAA
                     EVYGLGLDYIERYTSIVRGVTREAAAAAARAYLRPASGYALVVAGSVAAPGSAVGP"
     gene            complement(5044..>5388)
                     /locus_tag="VFM00_08400"
     CDS             complement(5044..>5388)
                     /locus_tag="VFM00_08400"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HET7498306.1"
                     /translation="SESAVRDAQRNADRNRIENVRFIRAEAAEALREERGPADAVLVD
                     PPRAGLHPVVARRLIEIGAPRLAYVSCNPATLGRDLALFCESRYRLSWVRPVDMFPHT
                     AHIECVAALARV"
BASE COUNT          760 a         1859 c         1881 g          888 t
ORIGIN      
        1 cggtcgttgt catgatcgtc atcgcgcccg ttcgaacgct gcatttcccg ccgcagcgac
       61 tggatctcct gccggagctc ccgcagctga tccctgaggt cccggatctg ctgctggacg
      121 tcccggtcgt tcatctggga cggtggcggc atcgggtggt cgtcgtcgcc ggtgcggaac
      181 cggatatccg gcatgcccat cccgggcatc atccccgcgg ggcgctcgcc aagctgcacc
      241 gtaaccgtac ggagagagcc cgagcgccag agcgtgaccc gtgcccggct gcccggcgaa
      301 agcccgcgca cgaccttgat caggtcctgg gagctgtcga tcttctcgcg gtccacctcc
      361 gtgatcacat cgccgcgccg gatccccgcc tgatccgcgg ggctctcgtc ctcgacgcgg
      421 ctgaccaggg cgccttcgtc ggtcgtcagg cctcgcgcgc gctggagcgc gcgggtgacg
      481 tcctggacct gcacgcccag gtagccgccc tgcgcgtcgt cgcccccgta gatcttgacg
      541 gtgccctggc ggccattgtc gtcgtcgtcg tcatccgccc tcgccgccac tggggcggtc
      601 aggagcgtga gcgccaggcc gagagccgcc aggctccaga ccggccgcat gaatcgaggc
      661 aatctcatcg tgttcctccg tatcgaaaaa aagggatggg gttgcgccgc gcctcactcc
      721 cgtctaccgc gaggaccggc cgaagttccc gcccttcctt ggacggtgga actagttggc
      781 cggttgaagc cggaatgacg agcagcccgc ctttccgatg aacgcgccga tcggaccaca
      841 cggcgcacgg agcgcgggcg acagatcgcg gagggcgaag ttgccgtgct ccgcgtcgca
      901 gaagagcggg tccccgaccc acgtgttgac ggaggggagc gcgccaatgg cccctccgga
      961 cggatcccag atgaggttgc agtcatatcg aggagacgtc tgcagccccg tcacggcgcg
     1021 ctgaccatga tccaccacga agatgttctt gcgcacctcg ggagagacgt cggtgaagaa
     1081 gagggtggcg ccgtctccct tgacatggtt ctcgaagaac gtgttgctct ggagcgtggg
     1141 attgatgttg ctcatgccgc gtcccccgat tcccatggcc gcgccgaacg cgagggacga
     1201 attctggttg aacaggcaga gccccatgtt catgcgcgag tgatcgcact gcaccgcgcc
     1261 gcccgctccg gcctgattct tctcgaaagt cgagcgggag atgttgaagg ccgagccgcc
     1321 tgagatgccg atcgcgccgc cggcgacgtc ggagctgttc tccttgaagg agcagttcac
     1381 gacggttccc tggctggaat cctcggcgca gacggcgccg ccgacggaac cgacggcccg
     1441 gttccgctcg aacacgaggt tgcggagggg gacgaggagg cgcgtccgac tgagcgagat
     1501 ggcgccgcct tcgtgccctt cgttgtcgta gaacttcgac tcgtagatcg ccaccaccga
     1561 gttgttggcg tcgatggcgc tccccttgtt ctcccgcagc gtgcagccgc cgatgacgat
     1621 cccctgtgag tcccagatcg agaccgccga ttcccagttg ccggtgaaga cgcagtcgac
     1681 gatctggacg gtgctgtccc gcaggcagta gatgccgcct ccgctgttcg aatggccgcg
     1741 ccggatcgtg aagcccagga cgctcacggc gttcgtcccc tcgatgtgca ggacccgaac
     1801 gctgtcccgt ccgtcgagga tcgtcttctc ggcgcccccc tccgcaagga gaacgagggg
     1861 cttcgtgatc cggaacggcc ccgggtagac gccgggggcg acccggatcg tgtcgccccg
     1921 cgccgcggcg tcgattctcg cctgcagcgc ctgcagcgcc ctgggcggtc ggggaacggc
     1981 gcgtcgcttg gtcctggctc cgcgcggcgc cgcggccgtc aggacgagcg cgagcaggat
     2041 cagcgcggcg agcgcggccg tgttcgccgc gccgccgtgg cgccggaccg gattcgtcgg
     2101 acgggcccta gggaccaacg gcgctccccg gggccgcgac cgatcccgcc acgaccaggg
     2161 cgtagccgga ggcggggcgc aggtaggcgc gggccgccgc ggcggccgcc tcgcgcgtca
     2221 cgccgcgcac gatcgaggtg tagcgctcga tgtagtccag ccccagtcca tagacctccg
     2281 ccgccacgag ctgcgacgcg acgccggcgt tggtctcgag acgcaccggg aacacgccgg
     2341 tcagatagtc cttggcatcc tccatctccg cgtccgtcgg accctgctcc gccatgcggc
     2401 gcacctgctc caggatgctc gcgaccgccc gatcggcgtt cgcgggattc gtcccggccc
     2461 ggatcgagag cgggcccgcg ccgatcccgg ccgcgaacga ggagtagacg ccgtacacca
     2521 gcccctgctc gtcgcgcagg ctgttcatga gccggctgga gagcgagccg ccgccgagga
     2581 tgtagttcat caccatcgcc gcgtcatagt cgggcgccgt gcgggggatg ccgggcaccg
     2641 cgtagacgac gtcggtctgt gacttgccct tcatcggcac ccgcagctcc agcgtcgtcg
     2701 ccggcgcttg cgccttggga acctcgaagg gcggggcggg ctcgagcttc gcccatccgc
     2761 cgaaggcggc gcgaaccagc tccaccgcgc gcttcgtgtc gacgtcgccg accaggacga
     2821 ggatcgtgtt gttgggcccg tagtggctcc ggtggaacgc cgccaggtcc tcgcgggtga
     2881 tcgcgcgcag cgtggtgtcg gtgcccatcg ggttcacgtg gtagggatgc cccttgggat
     2941 agaggaggcc gttcgcgcgg cgcgccgcca cgctggccgt cgagttctcg gcgacctggt
     3001 agtcggtgat ccgttccgcg cggatcttct ccatcttgtc cgcggggaag gcgggatgct
     3061 ggagggcgtc ggccagcacg tcgagcaccg tcggcagatc ggcggagagc atcgtggccg
     3121 agatcgaaac cgtctcggac ccaccgtcga acgagaggtg cgcgcccgcg ccctcgatcg
     3181 tctcggcggt ctggagcgcg gtgcgcttcg ccgtcccctg atcgagcatc gcggcggtga
     3241 atcccgccag cccgctccgc tccggcggat cgtagatgcc gcccgccttg acgagcccct
     3301 ggagggagat ggtgggattg gccgggttgt tccggacgat cagcgtgagc ccgttgggga
     3361 gaaccgtgcg atcgggggcc gccccatgca tgacgccggc cggggcggcg gccgccgcgc
     3421 ccggagcggc cgcgttcatc gcgcgctcgt cgggcatcgc ctcggagacg gaggcgggaa
     3481 ggccggggag cgcgtcggcc gcgtccccgg gccggtaatg atccacccac cgcgaggggg
     3541 tcgccgctcc ggccgcctcg gcgggagcgc cgtcccccgc gtcggcctgc ccttcgggcg
     3601 tcgggtcata ccagcccacc gtgcgcgagt tctccgtgag ataggtgcgc gccacccgct
     3661 gcaggtccgc ggtggtgacc gcgcggaccc gctccacgta ggtgtccaga tagcgccagg
     3721 aagctacggt gttgtagtac cccagctgct gcgccaggct ccggatcgag tccttggaat
     3781 agatgaacga ggcctcgacc tgcttcttgg cccgctccag ctccttggcg gtgaccggct
     3841 cgctcttgag ccgctccacc tcgtccagga gcgccgcctc caggcggtcc agccccaccc
     3901 ccggcgccgc cgtcgcctgc gcgttgaaca ggagcggatc gcggccgttg tcgttgctcg
     3961 cgtccacctc ggccgcgagc ttcttctcga ccagcgcctg gtagaggcgc gaggtctggc
     4021 cgtggcccag gatgataccc agcacatcga gggcgtaggt gtcggggtgc gtcaccgcgg
     4081 ggtttctcca ggccgcgagc aggtagcggg tgtcgccggg cttccggatc ttgaagcggc
     4141 gctcgcccat ctgcggcggc tccaccgtcg tgaccggcgg cggctcggtt cccttcggaa
     4201 ccgcgccgaa ataccgcttc accatggcga gcgccgcctg cgtgttcacg tcgccgacca
     4261 ccacgacggt cgcgttgttc ggcatgtagt ggctcgcgta gtagtcgcgg atctggctgg
     4321 tccggatctg ctcgacgtcg gttcgccagc cgatcgtggg ccagtggtag gggtgcgcct
     4381 tgaaggcctg cgccatcgct tcgagataga gggcgcgatg cgggttgtcc tcgttccgct
     4441 ccagctcgga gcggaccacc gtcatctcca gccggtgctc gtcgtcgggg atcgtcgcgt
     4501 ggaccatgcg gtccgcctcg atcttcatcg ccatctccag gcgatcgctg gcgagaacct
     4561 cgaagtaggt cgtgtagtcc agcgacgtgc ccgcgttgaa cgtggctccg ttcttctgga
     4621 gcaggcggga gatctccccc tttccgtacg tgggggtgcc cttgaacatc atgtgctcga
     4681 gcaggtggga gatgccggtg atgccggcgc tctcgttccg ggagcccacc ttgtaccaga
     4741 ccatgaaggc ggccaccggc gccgtgtgaa cctcctggat caacaccgtg agcccgttgt
     4801 cgagggtcgt ctcgagcacg ggcgacttcg ccgctggggc tgtcatggaa cgtttccctt
     4861 tagaagtggc gccggcggaa gccggcggtg cctggatcgc cgtgagtgcg agcagggaga
     4921 ggagaagtcc cgctccgcaa agccgcggtg tcgcgctcgg tcgaacgttc gaaacctgcc
     4981 gcattccgtc ctcccgggcg ccgcagcgcc caagcgtagc atgggacagc gtcgccgcgg
     5041 gtatcagacc cgcgccaacg ccgcgacgca ctcgatgtgc gcggtgtgcg gaaacatgtc
     5101 gaccggccgg acccaggaga gccggtaccg cgattcgcag aagagcgcca ggtcgcgccc
     5161 cagggtcgcc gggttgcacg agacgtaggc gaggcgtggc gcgccgatct cgatcagccg
     5221 ccgcgccacg acgggatgga gccccgcgcg cggcggatcg acgagaaccg cgtccgcagg
     5281 gcctcgctcc tcgcggagcg cctcggcggc ttccgcgcgt atgaaccgga cgttctcgat
     5341 ccggttccgg tcggcgtttc gctgcgcgtc gcgcaccgcc gactcgga
//
LOCUS       DASSLZ010000220         6597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1473859, whole genome shotgun sequence.
ACCESSION   DASSLZ010000220 DASSLZ010000000
VERSION     DASSLZ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473859"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..515
                     /locus_tag="VFM00_08405"
     CDS             240..515
                     /locus_tag="VFM00_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498307.1"
                     /translation="MQRFLVEVPHEAEMIACARAVKLLLETGSHFMTHADFGCRDGDH
                     RAWIIVEAENKDQARNMIPPLYRGPARVIGLNKFSIDEIDDLITRHS"
     gene            complement(529..1170)
                     /locus_tag="VFM00_08410"
     CDS             complement(529..1170)
                     /locus_tag="VFM00_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498308.1"
                     /translation="MSRLLWSFLSAAVILAIAPPPAAAGHEPANRRTHPSRDEGELGW
                     VLVSDGITSMSDSNDLESVDALRARYGDRFLLLRDGNDRYLIRDEGLIDRAEHATRSI
                     KLYGKEIGLLARYRAGDAFGKKSSRKIAAMAQRGPRDASDARLEREVEELIRDMERFG
                     AASRPADAARSRDLERRSDVVSKQLHEAVRQGQEEMRQILEDAKTRDLAKQAD"
     gene            complement(1260..1718)
                     /locus_tag="VFM00_08415"
     CDS             complement(1260..1718)
                     /locus_tag="VFM00_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498309.1"
                     /translation="MTGCTTKRERVEQRQQTSDKSPITTPPDGASRGISPTPDRAVVD
                     TVSSVAEIFSRIHEYESTLSQIIAAGRLDELGADASRISELLRTAGRLVQVSPDQRSN
                     FESHVSAAERAADALGEAGTAGNLEESKARNADLQRELGIVERLAHPGTS"
     gene            complement(1817..2257)
                     /locus_tag="VFM00_08420"
     CDS             complement(1817..2257)
                     /locus_tag="VFM00_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7498310.1"
                     /translation="MGTIAHVLTAKGPQVHTIRPTDTVFEAVKKMVELNSGSLLVTEG
                     DAIHGIITERDYLRNIVLAGRTSKTTQVREIMTSQIVVVKPDTLVEEAMAIMTDRRIR
                     HLPVVDQGKLGGIVSIGDLVKQISQDRQFEVQYLTDYITGRYPG"
     gene            2471..2674
                     /locus_tag="VFM00_08425"
     CDS             2471..2674
                     /locus_tag="VFM00_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009807287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HET7498311.1"
                     /translation="MTVARVTEITAESPKSFEDAIRIGIERANKTLENVKGAWIQEQK
                     LKIEGGKITAYRVNMKVTFILNA"
     gene            complement(2737..3249)
                     /locus_tag="VFM00_08430"
     CDS             complement(2737..3249)
                     /locus_tag="VFM00_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7498312.1"
                     /translation="MLTAAQRVLVLRGADLLRGVAQRRLLELAEVAREVELSKGGTIY
                     REEDAADALYMMVEGRVRLSVGDRIIADVGPGEAFGTWAIVDDAERGQRAECIEDGLA
                     LAVHRDDFYDVAAGDLRVLQEVVRVLARRLRALVSERPEEARVEGEGIEKPEALVKTE
                     TLESDAAASP"
     gene            complement(3243..5861)
                     /locus_tag="VFM00_08435"
     CDS             complement(3243..5861)
                     /locus_tag="VFM00_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HET7498313.1"
                     /translation="MAALVALLLCAYTIAKVLRDALFLAEFGALALPYAYLAVAVASV
                     GFVWVESHVARRFQRMGAARFNQYLAIACSIAAALVFPHARRLTTALFYVWTGSQAMM
                     LLPHFWGLALDVWDSRRARLLFPLLAGSGLIGGLAGGTLAGWSLPLVQRIGLMWTLPA
                     LLVLAHALTRAVERHRSRKPGPEVVPSTDSPWKILRRSPYIRVFVAALTLSVVVGTLV
                     DFQFKYYVLRLYPDPHDLTQFLGKFYVVLNALSLLFQFGMAGWLLQRLGLGISTGLQP
                     ATVLLFASWITFGPGLWVVAAMRSIQGVLSGTIGKSTGEIYYTPIRPSERRRIKPAID
                     TLVERWSDAAVGLLLIVAFHALHLPVSTIAILTAVLCGSWVVVLYGLDRQYGMEFRQV
                     LSTRWIEPDTPSEALRLPAARRALLEALRAEDEDRIVLALSLSGEIHDPAILRAVRQC
                     LGHRWPGVRAAAVSAMAAMHVADRENRIVTFLDDPHEGLRRAAVDYLLRRGPNRLAFA
                     RRILDGEDVALRRFAIDALYEHPCEARAALTLPWIDARIESGDPDSLVLAARALGVMR
                     GAGLVPRLRALLTLPDIDVRDAALHSAARRPAHALLDALLPLLFEPDLHYAARAAIAA
                     IGDRAVPALLRMLDEERGPRAQSLATRVLAQIDTRGAVDALMTMVRSRNLRLRHLGLM
                     GLTRMRARRGRPVLPRGMAHRLFLRELRDYRQCLDPARALEAHPAPEVRLLADSYRES
                     AEMALERALQALSCWYDPRPLAGAFDRLKSRDRATVSPALEFLEHVLPRASFQPVRQI
                     FEDAPAPPAEDAEGSDPLAVWIEGAWKSEDDWLRACAVRASRSLGAFDPTLFKGDPGA
                     PMVRDELALLRSSGPC"
     gene            6251..>6597
                     /locus_tag="VFM00_08440"
     CDS             6251..>6597
                     /locus_tag="VFM00_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498314.1"
                     /translation="MNVPMKSRLLAPVLAMSMVLVLAASAGAQDRRYERSQSPGVPLQ
                     IDFGSSPHWSRVPGTQVRAIRQGDRTDYDIFQYGRSYYAYNNANERWYMSRRWRGQFR
                     LIDDRSVPFELRRV"
BASE COUNT         1186 a         2186 c         2211 g         1014 t
ORIGIN      
        1 gatggctaag gagcatcgcg agaagaagaa ggagaaggac aaggatcagg aggcggacaa
       61 gacgtactga ttccgcgtcg aaatctcttg aggttccgga tgagagcccc ggccgcgaga
      121 cctgtggagc ttgtcgacca cgtacgacgc gctgcaatcg gcctgtccgc tgccgattgc
      181 cgatgcccgt tgggcgatgc tatcgtgacc cgaagagtcc acttccgagg agggtgacga
      241 tgcaacgatt cctagtcgaa gttccccacg aggcggagat gatcgcgtgc gcccgggcgg
      301 tgaagctcct gctcgagacc ggatcccact tcatgacgca tgccgacttc ggatgccggg
      361 acggcgatca ccgcgcctgg atcatcgtcg aggccgagaa caaggaccag gcccgcaata
      421 tgattccgcc gctgtaccgc ggccccgcac gggtcatcgg cctgaacaag ttctcgatcg
      481 acgagatcga cgacctgatc acgcgccaca gttgagggtg cggcgccgct agtcggcttg
      541 cttcgccaga tcccgcgttt tcgcgtcctc gaggatctgg cgcatctcct cttgcccctg
      601 gcgaacggct tcgtgcagct gtttcgagac gacgtcgctg cgccgttcca ggtcgcgtga
      661 tcgcgcggca tccgcagggc gcgaggcggc cccaaacctc tccatgtcgc gaatcagttc
      721 ctcgacctcc cgttcgagcc gcgcatcgga cgcgtcgcga ggtccccgtt gggccatggc
      781 ggcaatcttc cgcgagctct tcttgccgaa tgcatcccca gctcggtaac gcgccaggag
      841 accgatctcc ttcccataaa gcttgatcga gcgggttgca tgctcggcgc gatcgatcag
      901 cccctcgtcc cggatgagat atcggtcgtt cccgtcacga agcagcagga aacgatcccc
      961 gtatcgggcc cgaagcgcgt cgaccgattc gagatcgttc gaatcggaca tgcttgtgat
     1021 cccgtcggac acgaggaccc acccaagctc gccctcatca cgggagggat gggtccgccg
     1081 attcgcgggc tcatgaccgg cggctgcagg cgggggagcg atggcgagaa tgaccgcggc
     1141 ggacaggaaa ctccagagca ggcggctcat gggacaactc cagcgagggg tacgaagcgc
     1201 ggcctcagga ctcgccaggt tggatggtgg agagggccgg tgcgttgaaa ggcggtcaat
     1261 cagctggtcc cggggtgcgc gagccgctcc acgataccca gctcgcgctg gaggtcggcg
     1321 ttccgggctt tggactcctc caggttgccg gctgttccgg cctccccgag cgcatctgcg
     1381 gctcgctcgg ccgcggaaac atgactctcg aaattgctcc tctggtcagg agaaacctgc
     1441 accagacgtc cggccgtcct caggagctcg ctgattctcg acgcgtccgc acccagctcg
     1501 tccaggcggc cggcggcgat gatctggctc agcgtcgact cgtattcgtg aatccggctg
     1561 aagatctcgg ccaccgagct caccgtgtcc actaccgcgc ggtccggcgt cggggaaatg
     1621 ccgcgcgacg caccgtccgg cggggtggtg atcggagact tgtccgacgt ctgttgtctt
     1681 tgctccacgc gctcccgctt ggtggtgcaa ccggtcatgg ctccagcggc tacgagagga
     1741 acgcagaccc acgccaagaa atgctttcga aagctcacgg gtctctcctg aagttcgaca
     1801 ggagcccggc gcgatgctag ccgggatacc tgccggtgat gtaatcggtg aggtactgga
     1861 cttcgaactg tcggtcctgc gagatctgtt tcacgagatc gccgatcgac acgatccccc
     1921 cgagcttccc ctgatcgacg acggggagat gccggatgcg gcggtcggtc atgatcgcca
     1981 tcgcctcttc caccagggtg tcgggcttca ccacgacgat ctggctcgtc atgatctccc
     2041 gcacctgggt cgtcttggag gtgcggccgg cgagcacgat gttgcgaagg tagtcccgct
     2101 ccgtgatgat gccgtggatc gcgtctccct ccgtgacgag aagcgacccc gagttgagct
     2161 ccaccatctt cttcacggcc tcgaacaccg tgtcggtcgg gcggatcgtg tggacctgcg
     2221 gacctttcgc cgtgagaacg tgggcgatcg ttcccatcgt ggacctcctt ggcggggatg
     2281 gacgaacgtt gaacttctcc ctcccagggc caattcgacg gtactccttg gcggaccggc
     2341 tagccagtgg gggattggtg ccgtggaaag cccgcgtagg actacgcagt ggactcgacc
     2401 ccctgctttg cgtttcggcg gcgcccctgt agtgtggagt cttgttgcca ctcactcaag
     2461 gagcgacgcc atgacggttg cgagagtcac tgagatcacg gccgaatccc ctaagagctt
     2521 cgaagacgcc atccggatcg gaatcgagag ggcgaacaag accctggaga acgtgaaggg
     2581 agcctggatt caggagcaga agctgaagat cgaggggggc aagatcacgg cataccgtgt
     2641 gaacatgaag gtcacgttca ttctgaacgc gtaggctgga accgaagtcc gagcaggccg
     2701 agccggcccc ggtggcgaaa gctgccgggg ccgtcgctag ggggacgcag ccgcgtcgga
     2761 ctcgagcgtc tccgtcttca ccagggcctc gggcttttcg atcccctctc cttcgacgcg
     2821 cgcctcctcc ggacgttcgg acacgagcgc gcggagccgg cgcgccagca cgcgcacgac
     2881 ctcctgcagt accctgaggt cccccgccgc cacgtcgtag aaatcgtcgc gatgcacggc
     2941 gagcgcgagc ccgtcttcga tgcactcggc acgctggccc cgctcggcgt catccacgat
     3001 ggcccacgtc ccgaacgcct cgcccgggcc cacatcggcg atgatccgat cgccgacgga
     3061 aaggcgcacg cggccctcca ccatcatgta gagggcgtcg gccgcgtcct cctcgcggta
     3121 gatcgtccct cccttcgaga gctcgacctc acgcgccacc tcggccagct caagcaggcg
     3181 gcgctgggcc acgccgcgca ggaggtccgc gcccctcagg accaggacgc gttgggcggc
     3241 ggtcagcatg gcccgctcga gcgcagaagt gcgagctcgt cgcgaaccat cggcgcgccg
     3301 gggtcgccct tgaagagggt cgggtcgaac gcgcccaggg atcgcgaggc gcgtacggcg
     3361 caggctcgaa gccaatcgtc ctccgacttc cacgccccct cgatccagac ggcgagcggg
     3421 tcgctgccct ccgcatcttc tgcgggcgga gccggggcat cctcgaagat ctggcgcacc
     3481 ggctggaacg aggcgcgcgg gagcacgtgc tcgaggaact cgagcgcggg ggagacggtc
     3541 gcgcgatcgc gggacttgag ccgatcgaac gcgccggcaa ggggacgcgg atcgtaccag
     3601 cacgacagcg cctgcagcgc gcgctccagc gccatctccg ccgattcccg atagctgtcg
     3661 gccagaagcc gtacctcggg cgcgggatgg gcctcgaggg ccctggccgg atccaggcac
     3721 tggcgatagt cgcgaagctc gcggaggaac aggcggtgcg ccatgccgcg cgggagcacc
     3781 gggcgccccc tgcgcgcgcg catgcgggtc agacccatca ggccgaggtg gcgcagcctg
     3841 agattcctgc tccgcaccat cgtcatcagc gcatcgaccg cgccgcgcgt gtcgatctgc
     3901 gccagcactc tcgtggcgag cgattgcgcg cgcggtccac gctcctcatc gagcattcga
     3961 aggagcgcgg gcaccgcgcg gtcgccgatc gcggcgatcg ccgctcgcgc cgcgtaatgc
     4021 aggtccggct cgaacagaag agggagaagg gcgtccagga gcgcatgggc cggtcgccgc
     4081 gccgcggaat ggagcgccgc gtcgcgcacg tcgatatccg ggagggtcaa gagcgcgcgc
     4141 agcctcggga ccaggcccgc gcccctcatc acgccgagcg cccgcgccgc cagaacgagg
     4201 ctgtccggat cgcccgattc gatccgcgcg tcgatccagg gaagggtcag cgcggcccgc
     4261 gcctcacagg gatgctcgta gagcgcgtcg atggcgaatc gccgcaacgc gacatcctcg
     4321 ccgtccagga tccgccgcgc gaacgcgagg cggttgggtc cgcgccgaag gagatagtcg
     4381 acggccgcgc gccggaggcc ctcatgggga tcgtcgagga acgtgacgat ccgattctcg
     4441 cggtcggcga cgtgcatggc cgccatcgcc gagaccgcgg ccgcccgcac gcccggccac
     4501 cggtgcccga gacactggcg cacggcccgc aggatcgcgg ggtcgtgaat ctcgccgctc
     4561 aagctcagcg cgagcacgat acggtcctcg tcctcggcgc gcagcgcttc cagcaatgcc
     4621 cggcgcgcgg cgggaaggcg cagagcctcg gagggggtgt ccggctcgat ccagcgcgtg
     4681 ctcaacacct gccggaactc catgccgtac tgccggtcca acccgtagag cacgacgacc
     4741 caggagccgc aaaggaccgc ggtcaggatc gcgatcgtcg acaccggaag atgcagcgcg
     4801 tgaaacgcga cgatcaagag aagaccgacc gcggcgtcgg accaccgctc cacgagagtg
     4861 tcgatggcag gcttgatgcg gcgccgctcg ctggggcgga tgggcgtgta gtagatctca
     4921 ccggtcgatt tcccgatcgt tccggacagg acgccttgga tggaccgcat cgcagccacg
     4981 acccaaagcc ccgggccaaa ggtgatccac gatgcgaaga ggaggaccgt tgcgggctgg
     5041 agccccgtgg agatgccgag gccgagcctc tggagcagcc atcccgccat cccgaattgg
     5101 aacaggagcg agagcgcgtt caggacgacg tagaacttac ccaggaattg cgtgaggtcg
     5161 tggggatcgg ggtagagccg caggacgtag tacttgaact ggaagtcgac gagcgtcccc
     5221 accaccaccg agagcgtgag cgcggccacg aagactcgga tgtagggtga gcgccggagg
     5281 atcttccagg gcgagtcggt ggaaggcacc acctccggcc ccggcttgcg cgagcgatgt
     5341 cgctcgacgg cgcgggtgag cgcatgggca agaaccagaa gtgcggggag cgtccacatc
     5401 aacccgatgc gctggacgag cggcaaggac cagccggcaa gggtgccgcc ggcgagtccg
     5461 ccgatcaggc cgctgccggc gaggagcgga aacaacagcc gcgccctccg ggaatcccag
     5521 acgtccaggg ccaggcccca gaagtgcggg aggagcatca tcgcctggct cccggtccag
     5581 acgtagaaca gcgccgtcgt caggcggcgc gcgtggggaa acaccagggc ggctgcgatg
     5641 ctgcacgcga tggccaggta ctgattgaaa cgcgccgcgc ccatgcgctg gaaccggcgc
     5701 gcgacgtggc tttcaaccca gacgaatccg accgatgcga cggcgacggc aaggtaggcg
     5761 tagggcagcg cgagcgcccc gaactccgcc agaaagagcg cgtcccggag aacctttgcg
     5821 atcgtgtacg cgcagagcag cagcgcgacc agcgcggcca tgaggaggaa tcgtcggccc
     5881 tcctcgggct cgaggctcag ccttcgcgcg atggcccccc atccgcgagc gcgaatcaag
     5941 gccacaagcg ttgccctcac gggttcgtga tcgcaagcgc cgggcgagag cacgacgctc
     6001 gaattctcca gggtagacca taacggcggg cccgacgtga ccttgcgtcg agacctttac
     6061 ggaatcgaac gcgcaaccgc gtagacctac ggatgcacag atagcgcttg cgcttttcgc
     6121 ggtgtaagct aacatagctt acatgagcgc gaatacacct cgtccaacga cccggagtca
     6181 ccggcgaacg cacgaggccc gaccgtcgct ttcggtaggc tcgagcgctt tatcacgaag
     6241 aggagatccg atgaacgttc ctatgaagtc ccgcctcctg gccccggttc tggccatgag
     6301 catggtcctg gtcctggccg cgtcggccgg cgcgcaggat cgccggtacg agagatcgca
     6361 gagtccgggc gtccccctgc agatcgactt tgggagctca ccgcattggt cccgcgtccc
     6421 cggcacgcag gtccgggcga tccgtcaggg agaccgcacg gactacgaca tcttccagta
     6481 cggccgcagc tactacgcct acaacaacgc caatgagcgg tggtacatgt cgcgccgctg
     6541 gcgcggacag ttccgcctga tcgacgaccg ctccgttccg ttcgagcttc gtcgcgt
//
LOCUS       DASSLZ010000221         4321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1602317, whole genome shotgun sequence.
ACCESSION   DASSLZ010000221 DASSLZ010000000
VERSION     DASSLZ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602317"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..212)
                     /locus_tag="VFM00_08445"
     CDS             complement(60..212)
                     /locus_tag="VFM00_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498315.1"
                     /translation="MSSGSNAEPEGELADEAEANDEDTVAKDDVPGADAMEGDGPDGG
                     EGRVGE"
     gene            222..1859
                     /locus_tag="VFM00_08450"
     CDS             222..1859
                     /locus_tag="VFM00_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7498316.1"
                     /translation="MDPRRDRVDLALQSGAERADTALDSSTAQQILDWSSGRGADVVL
                     ITAGGADNQPLVLAGAAARDRAKVVVVGAVDLDVPREPFYEKELSLTVSRSYGPGRYD
                     PSFEEKGMAYPVGFVPWTERRNMEEFLTLLANRRLTLEGLKPVTIPFERAPEGYELLS
                     GKNGPSPISVVLEYAPAPSANGDAPSEMAAVPSLPGFLADAPVAPVEPPAAPRARRRF
                     KPRALQVSFLGLGNFASTYLLPPVKASGASLERVITATPLKAETAKRRAHFRVAGTVA
                     DDAIDDPRTEVVIIATRHDVHAEYAVKALRKDRAVFVEKPLALTTEELQAVKAALHET
                     QGRLMVGFNRRFAPATTWALDALGPNSAGLRVLIRVNAGVLPHRHWLLDHESGGGRLL
                     GEACHFIDYACYVAGSAPTQIEARALDAPQEETGHQSYRVDLLFENGATATIDYLANG
                     DSSLPKERIEIHRSGTSLVIDDFRSASIHRAGKRRTKSWGARDKGHKTEVKAFLEAVR
                     TGAPTPIPEEESILSTALTLAAARSIREARPIRREEW"
     gene            1861..3069
                     /locus_tag="VFM00_08455"
     CDS             1861..3069
                     /locus_tag="VFM00_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498317.1"
                     /translation="MTSADALHRILERAELDGWAGSDPYDALLSPLGRAVTRFGGLPR
                     FVLSQLVLRVPAARTFTNPPATVNTKGLALFLGAVTRGRHLLGPKRPRELARELIAEI
                     ESRATPAGGGLGWGYPFPWQSRSFWAPAGTPNAVVTVTVGWHALYAAEVFEDANARAL
                     GLGAARFLASGLNVTPVAPDAAAISYTAADRTRVVNVSALAGRLLLRAARVGGEPELA
                     VLAGKLLRFVLQAQRPDGSWPYATEKGGAWMDSFHTGYVLESLVHAREMGYPVPDGAL
                     SRGIEAYGRFFAPNGAARLTLPESSPFDAHSAAQGMVTYGAVAHSEAASDSRRHSARA
                     AAFAIADWSLRALWIPGESHFAYRIARGWRDEREFLRWVQAWMALGMATVESLRTVEA
                     TAPSMPVGVA"
     gene            3066..4127
                     /locus_tag="VFM00_08460"
     CDS             3066..4127
                     /locus_tag="VFM00_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HET7498318.1"
                     /translation="MRVWIDVDNAPHVQIFRPIIGRLREKGADVSVTARGRTFVPELL
                     QAASIEHTIIGRGQPRGAAAKATALAGRAAALARYASGERFDVAVGHGSRSLPPAARM
                     AGVPNLTMFDYEHVSTWLFRRFCDRILIPSAVATSVVGARPGDPWRTFEGFKEEIYLA
                     DFAPDRAIRRKLEIPEEEVLVVVRPPSRTAHYHDAASEAILDAVARRIGQGQGVRAIW
                     LRRDATEVVPDSARAANILVPDAPLDGLSLLAAADLAISGGGTMNREAALLGTPAYSI
                     FTGPAGALDQELIRTGRLTAVRDPEAVAAIPFVRKPEAPPFHYAGDLREHVVDQIWDL
                     GTHRTRAGAMGRKRWAFPS"
     gene            4109..>4321
                     /locus_tag="VFM00_08465"
     CDS             4109..>4321
                     /locus_tag="VFM00_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET7498319.1"
                     /translation="MGVPFVDLKAQYQSIQEEIDAAIREVVTSCAFVLGPAVERFERD
                     FAGFLGVEDVVGVNNGTAALQLTLLAL"
BASE COUNT          601 a         1533 c         1515 g          672 t
ORIGIN      
        1 accatgccga cctggttagc gtcactaaga atctcaccgt tcctgtccct tcgggcgtct
       61 cactctccga cgcggccttc accaccatcg ggtccatcgc cctccatggc atccgcaccg
      121 ggaacgtcgt ccttggcgac cgtgtcctca tcattggcct cggcctcatc ggccagctca
      181 ccctccggct ctgcgttgct gccggagctc atgttttcgg cgtggatcct cggcgtgatc
      241 gggtggatct ggcgctacaa tcgggcgccg agcgggcgga cactgccctg gacagctcca
      301 ccgctcagca gatcctcgat tggtcttcgg ggcgcggagc ggacgtcgtg ctcatcacgg
      361 cgggcggggc ggacaaccag cctctggtgc tggcgggggc cgcggcgcgg gaccgggcca
      421 aggtcgtggt cgtcggcgcg gtggacctgg atgttccccg cgagccgttc tacgagaagg
      481 agctgagctt gacggtatcc cgctcctacg ggcccggacg ctacgatccc tccttcgagg
      541 agaaggggat ggcctatccg gtcgggttcg tcccgtggac ggagcggcgc aatatggagg
      601 agttcctcac ccttctggcc aaccgccggc tcacgctcga ggggttgaag ccggtcacga
      661 tccccttcga gcgcgcgccg gagggctacg agctgctctc ggggaagaac gggccttcgc
      721 cgatctcggt cgtgctggaa tacgcaccgg ccccatccgc caacggcgac gctccgtcgg
      781 agatggccgc ggtgccgtcg ctgccggggt tcctcgccga cgcaccggtc gcccccgtcg
      841 agcctcccgc cgcgccgcgc gcacgccggc gcttcaaacc tcgcgcgctc caggtgtcat
      901 tcctggggct tgggaatttc gcttccacct acctgctgcc cccggtcaag gcctcgggcg
      961 ccagcctgga gcgggtcatc accgccacgc cgctcaaggc ggagaccgcg aagcggcgcg
     1021 ctcatttccg cgtggcgggc acggtggcgg acgacgcgat cgacgatccc cggacggagg
     1081 tcgtcatcat cgccacccgg catgacgtcc acgccgagta cgcggtcaag gcgcttcgga
     1141 aggaccgggc cgtctttgtg gagaagccgc tcgccctcac gaccgaagag ctgcaggccg
     1201 tgaaggcggc gctgcacgag acccagggcc gcctgatggt cggcttcaac cgccgcttcg
     1261 ctccggccac gacctgggcc ctggacgccc tgggcccgaa cagcgcgggg ctgcgcgttt
     1321 tgatccgcgt gaacgcgggg gtgctcccgc accggcactg gctgctggac cacgagtccg
     1381 gcggcgggcg gcttctgggc gaagcgtgcc acttcatcga ctacgcctgt tacgtcgcgg
     1441 gctccgcccc gacgcagatc gaagcgcggg cgctggacgc gcctcaggaa gaaacgggcc
     1501 atcagagcta tcgcgtcgat ctcctgttcg aaaacggcgc cacggccacg atcgactatc
     1561 tggccaacgg cgattcctcg cttcccaagg agcggatcga gattcatcgc tccggcacat
     1621 cgctcgtgat cgacgacttc cgttcagcct ccatccatcg cgcgggcaag cgccgcacca
     1681 agagctgggg cgcgcgcgac aaggggcaca agacggaggt caaagccttc ctcgaagcgg
     1741 tccggacggg ggccccgacc ccgattccgg aagaagagag catcctcagc acggcgctga
     1801 cgctggccgc cgcccgctcg attcgcgagg cgcggccgat tcggcgcgag gagtggtgac
     1861 atgacatcag cggacgcgct ccatcgaatc cttgaacgcg ccgagctgga cggctgggcg
     1921 ggatcggacc cctacgacgc gctcctctcc cctttgggcc gcgcggtcac gcgcttcggc
     1981 ggcctgcccc gcttcgtgct ctcgcagctg gtgctgcgcg tgccggccgc gcgcaccttc
     2041 acgaatccgc cggccacggt gaacacgaag ggacttgcct tgttcctggg cgcggtcacg
     2101 cgcgggcggc acctcctggg acccaagcgc ccccgcgagc tggcgcgcga gctgatcgcc
     2161 gagatcgaat cgcgggcgac gcccgccggc gggggactgg gctggggcta tcccttcccc
     2221 tggcagtcgc ggtccttctg ggcccccgcg gggacgccga acgcggtggt gacggtcacg
     2281 gtcggatggc acgcgctgta tgccgccgag gtgttcgaag acgcgaacgc gcgcgcgctg
     2341 gggctgggcg ccgcgcgctt cctggcctcg gggctcaacg tcacgccggt cgcgcccgac
     2401 gcggccgcca tctcctacac cgcggccgac cgcacgcggg tcgtgaacgt gtcggcgctc
     2461 gcggggcggc tgctgcttcg cgcggcccgc gtgggcggcg agcccgagct ggccgtcctc
     2521 gccggaaagc tcctccgctt cgtgctccag gcgcagcggc ccgacggatc gtggccctac
     2581 gcgacggaga agggcggggc gtggatggac tccttccaca cgggatacgt gctggaatcc
     2641 ctggtgcacg cgcgcgagat gggctacccg gtgcccgacg gcgcgctctc gcgcgggatc
     2701 gaagcctacg gccgcttctt cgctccgaac ggcgcggcgc ggctcacgct gcccgagtcc
     2761 tcgccgttcg acgcgcactc ggccgcgcag ggcatggtca cgtacggcgc cgtcgcgcac
     2821 tccgaggccg cgtccgactc gcgccggcac agcgctcgcg ccgccgcgtt cgcgatcgcg
     2881 gactggtcgc tgcgcgcgct ctggatcccg ggcgagtcgc atttcgctta tcgcatcgcc
     2941 cgcgggtggc gcgacgagcg cgagttcctg cgctgggtgc aggcgtggat ggcgctgggc
     3001 atggcgaccg tggagtcgct ccgcaccgtg gaagcgacgg ctccctcgat gcccgtgggc
     3061 gtcgcgtgag ggtctggatc gacgtcgaca acgcgccgca cgtccagatc ttccgtccca
     3121 tcatcggacg gcttcgcgag aagggcgcgg acgtctccgt gaccgctcgc ggccggacgt
     3181 tcgttcccga gctgcttcag gccgcgtcga tcgagcacac catcatcgga cggggacagc
     3241 cgcgaggcgc cgccgccaag gccaccgcgc tcgcgggacg cgccgcggcg ctcgcgcgct
     3301 atgcctcggg cgagcgcttc gacgtggccg tggggcacgg ctcccggtcg ctgccgccgg
     3361 cggcccgcat ggcgggcgtt cccaacctca cgatgttcga ctacgagcac gtctccacct
     3421 ggctcttccg gcgcttctgc gaccgcatcc tgattccgag cgcggtggcg accagcgtcg
     3481 tcggcgcgag gcccggcgat ccctggcgca ccttcgaagg attcaaggag gagatctatc
     3541 tcgccgattt cgctcccgat cgcgccatcc ggcgcaagct ggaaatcccc gaggaggagg
     3601 tgctggtggt cgtacgccct cccagccgaa cggcccacta tcacgacgcc gccagcgagg
     3661 cgattctcga cgcggtggcg cggcggatcg ggcaggggca aggggtacgg gcgatctggc
     3721 ttcgtcgcga cgcaacggag gtggtgcccg actccgcccg cgccgccaac atcctcgttc
     3781 ccgatgcgcc gctggatggg ctctcgctcc tggcggcggc ggacctcgcc atctcggggg
     3841 gcggcacgat gaaccgggaa gcggcgctcc tcggcacgcc cgcctacagc atcttcacgg
     3901 gtccggcggg ggcgctggac caggagctga tccgtactgg acgcctgacc gccgtgcggg
     3961 atccggaggc ggtcgcggcg attccgttcg tcaggaagcc ggaagcgccg ccgtttcact
     4021 atgccggcga ccttcgcgag cacgtggtgg accagatctg ggacctcggg acgcaccgga
     4081 cgcgggctgg ggcaatgggg aggaaacggt gggcgttccc ttcgtagacc tgaaagcgca
     4141 ataccagagc atccaggaag agatcgacgc ggccattcgc gaggtcgtga cctcgtgcgc
     4201 gttcgtgctg gggccggcgg tggagcggtt cgagcgcgac ttcgccgggt tcctcggcgt
     4261 cgaggacgtg gtgggcgtga acaacggcac cgccgcactc cagctgacgc tgctcgcgct
     4321 g
//
LOCUS       DASSLZ010000222         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_463341, whole genome shotgun sequence.
ACCESSION   DASSLZ010000222 DASSLZ010000000
VERSION     DASSLZ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463341"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFM00_08470"
     CDS             complement(<1..300)
                     /locus_tag="VFM00_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498320.1"
                     /translation="MFPRDLIQRSRLRPRRIVASVFLLTSVLFLAVPAAASVSTVRGV
                     TVSPAAPTACDSVTLTMQGTLTDTCQPIVGDQRIGPDPIPEWAGPLPAYKTILILN"
     gene            complement(451..1278)
                     /locus_tag="VFM00_08475"
     CDS             complement(451..1278)
                     /locus_tag="VFM00_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498321.1"
                     /translation="MKLHHLRTQLRYARSIPAALLVGGLAVIGLGGCGKDNVAAPHRV
                     PLTLEAQGSGAPAQRFSTAAMLSAASAQDTIPVTFTRAVITVRDIRFVLPEGEDHADT
                     LGLGDNDSTDAEGSDTGGQVRFHGPFVIDLLAGDAQSLGTQMVPAGVYSMIMGHLQAL
                     AAGGPLATQYPDLVGATILLEGDIAGDGGGHFVYSARIDDEFIIHGSFNVTGDKPVTT
                     FLVFDLSKLLMDRDGHFLDPRNLDNDQAIRQAIRHAIKMGVDRNRDGEPDDAMSEAD"
     gene            1434..>2660
                     /locus_tag="VFM00_08480"
     CDS             1434..>2660
                     /locus_tag="VFM00_08480"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET7498322.1"
                     /translation="MNPRIPRRPHVLLAAIAIALAAASIARADEARLPADVQPRFEAV
                     QLQLDPSKPDYTGTVRVDLTVRQPTETIRFHAQALDLRRVELAGANGKTLLAYGPDDH
                     GIVTAKATATITPGDYVLTIDFMNDFDRTAASLFRLETGGRWYAYTQFEAVDARKAFP
                     CWDEPAFKFPWQVTLSIPDGQEAVSNTPVEKDTRAGGVRTLVFAKTKPLPSYLIAMAV
                     GPLEFVPIPGLRVPGRVVVPRGSKALASTAAAMTPPLFDALERWFGIPYPYEKLDLIA
                     VPEFSPGAMENPGAITYGDQFLLFDEKSISASQKRTFAVFTAHEMAHMWFGDYVTMKW
                     WDDLWLNESFAEWMGDKIADQVYPAYRIPLHELSDGERAFTSDAQLATHAVRRPVKTM
                     DNLYEAADDLAYHKGQA"
BASE COUNT          434 a          867 c          933 g          426 t
ORIGIN      
        1 gttgaggatg aggatcgtct tgtaggcggg cagaggcccc gcccactcgg gaatcgggtc
       61 gggtccgatc ctctggtcgc ccacgatcgg ctggcaggtg tcggtgaggg tcccctgcat
      121 cgtgagcgtg accgagtcgc aggccgtggg tgcggcgggc gagaccgtaa cgccgcgcac
      181 ggtggacacg gaggccgccg ccggaacggc gaggaacagc acggaggtga gcaggaagac
      241 ggacgcgacg atgcggcggg gtcgaagccg ggaacgctgg atcaggtcac gagggaacat
      301 ggctgcaaag ccctcatgtc cggcggcacg gggaggccgg gggcggcgcc tccggctcgt
      361 ggttcgagac tctcgacgag tgtcctcctc gccgggaaaa aaatccagcc cgctccgaca
      421 gggtcgtcgc gggctggatc cctgtgctgg ctagtcggct tcgctcatcg cgtcgtccgg
      481 ctcgccgtcc cggttccgat cgacccccat cttgatcgca tgacggatcg cctggcggat
      541 ggcctggtcg ttgtccagat tcctcggatc gaggaagtgg ccgtcgcggt ccatgagcag
      601 cttggagaga tcgaaaacca ggaacgtggt caccggcttg tcgcccgtca cgttgaacga
      661 gccgtggatg atgaactcgt cgtcgatgcg cgccgagtac acgaagtggc cgccgccgtc
      721 tccggcgatg tccccctcga gaaggatcgt ggccccgacc agatccgggt attgggtcgc
      781 gagcggaccg ccggcggcga gcgcctggag gtggcccatg atcatggaat agacgcccgc
      841 cgggaccatc tgcgtcccga gcgactgggc atcgccggcc aagagatcga tgacgaacgg
      901 gccgtggaag cggacctggc ctcccgtgtc gctcccttcg gcgtcggtcg agtcgttgtc
      961 gccgagcccg agcgtgtcgg cgtggtcctc gccttcgggg aggacgaacc ggatatcgcg
     1021 aaccgtgatg accgcccgcg tgaaggtcac cgggatcgtg tcttgggcgg aggcggcgct
     1081 cagcatcgcg gccgtggaga agcgctgggc cggggcgccg gaaccctggg cttccagggt
     1141 gagcggaaca cggtggggcg cggcgacgtt gtccttacca cagcccccga ggccgatgac
     1201 ggcaaggccg ccgaccagaa gggccgccgg aatggagcgg gcgtagcgaa gctgggtgcg
     1261 gagatggtgg agtttcatgg tctgggttct cctggctgga tgtcgagcgt ggaccaatga
     1321 cccctgcgac tccccttgag tgtgccatta tagcacacct ctttgggctg gccggttccc
     1381 gggtgctcca tgcgatactc gcgttccatg ccgccgatag ggaggaatcc tcgatgaatc
     1441 cacggatccc gcgccgtccg cacgtcctgc ttgccgcgat cgcgatcgct cttgccgccg
     1501 cttcgatcgc gcgagcggac gaggcgaggc tgccggccga cgtccagccc cggttcgagg
     1561 cggtgcagct ccagctggac ccctcgaagc cggactacac cgggacggtc cgcgtggatc
     1621 tgacggtgcg gcaacccacc gagacgatcc gcttccacgc gcaggcgctg gacctgcgcc
     1681 gcgtggagct ggccggagcg aacggcaaga cgctcctggc gtacggcccc gacgaccacg
     1741 gcatcgtgac cgcgaaggcg accgcgacca tcacgccggg ggactacgtc ctcacgatcg
     1801 atttcatgaa cgacttcgac cggacggccg cttcgctctt ccggctggag accggcggcc
     1861 gctggtacgc ctacacgcag ttcgaggcgg tggacgcgcg gaaggcgttc ccatgctggg
     1921 acgagcccgc cttcaagttc ccctggcagg tcaccctctc catccccgat ggccaggagg
     1981 cggtctcgaa cacgcccgtg gagaaggaca cccgcgcggg gggcgtccgc acgctggtct
     2041 tcgcgaagac caagccgctt ccttcctacc tgatcgcgat ggcggtcggt cccctggagt
     2101 tcgttccgat tccggggctc cgcgtgcccg gtcgcgtcgt ggtgccgcgg ggatcgaaag
     2161 cgctcgcgag cacggccgcg gccatgaccc cgcccctgtt cgacgctctg gagcgatggt
     2221 tcgggattcc ctatccctac gagaagctgg atctcatcgc ggtgccggag ttctctcccg
     2281 gcgccatgga gaacccgggc gcgatcacct acggagacca gttcctgctc ttcgacgaga
     2341 agtcgatcag cgcctcccag aaacggacct tcgccgtctt caccgcgcac gagatggcgc
     2401 acatgtggtt cggagactac gtgacgatga agtggtggga cgacctctgg ctgaacgaat
     2461 cgttcgccga gtggatgggg gacaagatcg ccgaccaggt ctatcccgcc taccggatcc
     2521 cgctgcacga gctgagcgac ggcgagcgcg cgttcacgtc cgacgcgcag ctcgcgaccc
     2581 acgccgtgcg ccggccggtg aagacgatgg acaacctcta cgaggcggcc gacgacctcg
     2641 cttaccacaa ggggcaggcg
//
LOCUS       DASSLZ010000223         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_590093, whole genome shotgun sequence.
ACCESSION   DASSLZ010000223 DASSLZ010000000
VERSION     DASSLZ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590093"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFM00_08485"
     CDS             <1..864
                     /locus_tag="VFM00_08485"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET7498323.1"
                     /translation="SAHWNVSPEWIAVGSGSNDLIDVLCRIYLGPGDEAIMSHPAFVM
                     FAVAVKVAGGTLKRVPGRDLFHDPDAMLAGITSRTKLVYFSNPDNPTGTIVRRRAMDD
                     YFRRVPDHVLTIIDEAYFEYVTDPEFPNALDYLRQGKRVAVLRTFSKIYALAGLRVGY
                     GFFPEDLAAVVHRVRLPFNVTTVGQAAARASLADPEQVIRSRHANAEGISFLERALPG
                     RGIRVTPSWANFVLAKFPGSAVEATRTLERLGVIIRPMTSFGLPAEYARISVGTRLEN
                     DRLIEALAKAT"
     gene            861..1742
                     /locus_tag="VFM00_08490"
     CDS             861..1742
                     /locus_tag="VFM00_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET7498324.1"
                     /translation="MTERPETPDAPPRRVAILGLGLMGGSLGLALRAADPAARVLGFD
                     EDDAAARRALERGAATERLRDPGAVPAETEVVALAMPPDAAAGWVRGLADSVPPGAIV
                     MDLSSIMLPALAASLETPSLADRFVPAHPLAGSESSGIESARADLYRGAAVLIGLPLD
                     AGTPGGRAAALWRALGARPASIAPALHDALVALTSHLPYAASVALVRTLRRTGSMTRA
                     LAETAGPGLRDTTRVAGSPPALWAEILERNGPKLLPALELLEREVRALRHAIQEGGPA
                     LREHLEEARSFREEIVR"
     gene            1739..>2389
                     /locus_tag="VFM00_08495"
     CDS             1739..>2389
                     /locus_tag="VFM00_08495"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET7498325.1"
                     /translation="MSRAATRPGKPLRGETTVPGDKSVTQRAVLLGALAPGTTRIRGA
                     NPGADARAALGLARALGARVRTTRAEWILEGGTLAESETVLDAKNSGTALRLATGLLA
                     AQPFLSVLTGDALLRRRPVDRVIAPLRLLGADVHARSRDRLPPVVVRGTALHGAHVDT
                     KVTSAQVKSALLFAAIQASGTTIVEEPVATRDHTERMLPLFGARVVREGRAARVTGP"
BASE COUNT          300 a          890 c          862 g          337 t
ORIGIN      
        1 agtgcgcact ggaacgtgtc gcccgagtgg atcgcggtcg gctccggatc gaacgacctg
       61 atcgacgtcc tctgccgcat ctatctcggc ccgggggacg aggcgatcat gtcgcatccg
      121 gcgttcgtga tgttcgccgt cgccgtgaag gtggcgggcg ggacgctgaa gcgcgtgccc
      181 gggcgggatc tgttccacga tcccgacgcc atgctcgccg gcatcaccag ccgcaccaag
      241 ctcgtctact tctcgaaccc cgacaatccc accggcacca tcgtccggcg ccgcgcgatg
      301 gacgactact tccgccgcgt gcccgatcac gtgctcacga tcatcgacga ggcgtatttc
      361 gaatacgtca ccgatccgga gtttccgaac gcactggact acctgcgcca ggggaagcgg
      421 gtcgccgtgc tccgcacctt ctcgaagatc tacgccctgg ccggactccg cgtgggctac
      481 ggcttcttcc cggaggacct cgcggcggtg gtccatcgcg tccggctgcc cttcaatgtc
      541 accacggtgg ggcaggcggc cgcgcgggcc agcctggccg atccggagca ggtgatccgc
      601 agccgccacg cgaacgccga gggcatctcc ttcctcgagc gcgcgcttcc gggacggggg
      661 atccgcgtga cgccgtcgtg ggcgaacttc gtcctcgcga agtttccggg ctcggcggtg
      721 gaagccaccc gcacgctgga gcggctgggg gtgatcatcc gcccgatgac ctccttcggg
      781 ctcccggccg agtacgcgcg gatcagcgtc ggcacccggc tcgagaacga ccggctgatc
      841 gaggcgctcg ccaaggcgac gtgacggagc gcccggaaac cccggacgcg ccgccgcggc
      901 gggtcgcgat cctcggactc ggcctgatgg gcggctcgct gggcttggcc cttcgcgcgg
      961 ccgatcccgc cgcccgcgtc ctgggcttcg acgaggacga cgccgcggcc cgccgcgccc
     1021 tggaacgtgg tgccgccacg gaacgcctgc gcgatcccgg ggccgttccc gccgagaccg
     1081 aggtcgtggc gctcgcgatg cccccggacg ccgccgccgg ctgggtgcgc ggactcgccg
     1141 attccgttcc tcccggagcc atcgtcatgg atctctcgag catcatgctt cccgcgctcg
     1201 cggcgtcgtt ggagacgccg tcgctcgccg accgcttcgt gccggcgcat ccgctcgcgg
     1261 gctcggagtc gtccgggatc gaatccgccc gcgccgacct ctatcgcggc gcggccgtgc
     1321 tgatcgggct tcccctcgac gcgggaaccc ctggaggacg cgcggcggcg ctctggcgcg
     1381 cgctgggcgc ccggcccgcg tcgatcgctc cggcgctgca cgacgcgctg gtcgcgctca
     1441 cgagccatct gccgtacgcc gcgtcggtcg ccctggtgcg cacgctccga cgcacgggat
     1501 cgatgacgcg cgcgctggcc gagacggcgg ggccggggct gcgcgacacg acgcgcgtgg
     1561 cgggctcacc gccggccttg tgggcggaga tcctcgagcg gaacggtccg aagctccttc
     1621 ccgcgctcga gctcctggag cgcgaggtgc gcgccctgcg ccacgccatc caggaggggg
     1681 ggcccgcgct gcgcgagcat ctggaagagg cccggtcctt ccgggaggag atcgtgcgat
     1741 gagccgcgcc gccacgcgac ccggaaagcc gctccgcggc gagaccacgg ttccggggga
     1801 caagtcggtc acgcagcgcg cggtgctcct gggcgcgctg gcgccgggaa cgacgcggat
     1861 ccgcggcgcc aatcccggcg ccgacgcgcg cgccgcgctg ggcctcgcgc gggcgctggg
     1921 cgcacgcgtc cggaccaccc gcgccgaatg gatcctcgaa ggaggaacgc tcgccgagag
     1981 cgagacggtg ctcgacgcga agaactcggg gaccgcgctc cggctggcga cgggcctgct
     2041 cgccgcgcag ccgtttcttt ccgtgctcac gggtgacgcc ttgctgcgcc gccggccggt
     2101 ggaccgcgtc atcgcgccgc tccggctgct gggcgccgat gtgcacgcgc gctcgcgcga
     2161 ccgcctgcct cccgtcgtcg tccggggcac ggcgctccac ggcgcgcacg tggacaccaa
     2221 ggtgacgagc gcgcaggtga agagcgcgct tctgttcgcc gcgatccagg cttcggggac
     2281 gacgatcgtg gaggagccgg tcgcgacgcg cgaccacacc gagcggatgc tcccgctctt
     2341 cggcgcgcgc gtcgtccggg agggacgggc cgcgcgcgtg accgggccc
//
LOCUS       DASSLZ010000224         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1222111, whole genome shotgun sequence.
ACCESSION   DASSLZ010000224 DASSLZ010000000
VERSION     DASSLZ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222111"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1141
                     /gene="prfA"
                     /locus_tag="VFM00_08500"
     CDS             35..1141
                     /gene="prfA"
                     /locus_tag="VFM00_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET7498326.1"
                     /translation="MREALEKAKARFDELTRLLEDPAVHSNPSELKRVSKERSALDPL
                     IRAIRRYDRVLERIHDDSTLLRTEKDPELAAMAKAEIDELTVEKEQIEAELPRLLLPP
                     DPFDQKNVIVEIRAGTGGDEAALFAGEVLRMYTKYAERHGWRVEVLSISQSGGGGIKE
                     VSLLIAGDGVYRRFKYESGVHRVQRVPETEASGRIHTSAVTVAVLPEAEEVDVELKPS
                     DLQIDVFRSSGPGGQSVNTADSAVRIRHLPTGIVVQCQDERSQLKNKAKALKVLRARL
                     LDMEIQEQEKKLAASRKSQVSSGDRSAKIRTYNFPQGRVTDHRIGLTLYRLQDVMEGD
                     LTELTDALFAAEQAERLASSGEASGPSPRAGAGA"
     gene            1138..2046
                     /gene="prmC"
                     /locus_tag="VFM00_08505"
     CDS             1138..2046
                     /gene="prmC"
                     /locus_tag="VFM00_08505"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET7498327.1"
                     /translation="MRAGIRLSRAARTARRAGTSRAEALASLTRRFERAGIDSPEHDA
                     EAILLRALQVSRADLWTEPNAPLTEEEAEDLTSLAERRSAREPLQLLLGTTPFCSATL
                     DLERGVFLPRPETEGLVEAVLRALHADRGRLLELGAGTGAIAIAILHALPEWTGEAVD
                     RSPQAVRLARKNAARNGIARRLHVLEGDFRDPGALRVPGHDYDLVVSNPPYVRSGEIP
                     GLMPEVRDHDPIEALDGGPDGLDALRDLARGIPLWLRPGGLLALEIGADQADDSQELM
                     RPHLEEMRVLPDLAGRPRVLLGTRRR"
     gene            2049..>2693
                     /locus_tag="VFM00_08510"
     CDS             2049..>2693
                     /locus_tag="VFM00_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET7498328.1"
                     /translation="MEAKAAADTKATKGTATGMDEIWVRGGKRLSGSVAVSGSKNATL
                     PVLAAALLAPGVYRFTNVPALKDVATMLEMLGRFGVKSRRTGPSAVEVDTRGQVNAEA
                     PYELVKTMRASIYVLGPLLARYGEARVSLPGGCAWGPRPVDLHIRGMEALGADVAIEH
                     GYIVARSERLRGSNISFDISSVGATGNLLMAAATASGETVIENAACEPEITALAD"
BASE COUNT          422 a          944 c          977 g          350 t
ORIGIN      
        1 cgctccgcgc ggtcctctag ctccacccac ccccatgcgc gaagccctgg aaaaggcgaa
       61 agcccgcttt gacgagctga cgaggctcct cgaggatccg gccgtccact ccaatccttc
      121 cgagctgaag cgggtgagca aggagcggag cgcgctcgat cccttgatcc gggcgatccg
      181 gcgctacgac cgggtgctcg agcggatcca cgacgactcc accctgctgc gcaccgagaa
      241 ggaccccgag ctggccgcga tggccaaggc cgagatcgac gagctgaccg tcgagaaaga
      301 gcagatcgag gcggagctgc cgcggctgtt gctccccccc gatccgttcg accagaagaa
      361 cgtgatcgtc gagatccgcg ccggaacggg cggcgacgag gcggcgctct tcgcggggga
      421 ggtgctccgc atgtacacca agtacgccga gcgccacggc tggagggtcg aggtgctgtc
      481 catcagccag tcgggcgggg gaggcatcaa ggaagtctcg cttctcatcg cgggcgatgg
      541 ggtctaccgt cgcttcaagt acgagagcgg ggtccaccgc gtgcagcgcg tgcccgagac
      601 cgaagcgagc gggcggatcc acacctcggc ggtgaccgtg gcggtgctcc cggaggcgga
      661 ggaggtggac gtggagctga agccgtcgga tctccagatc gacgtcttcc ggagctcggg
      721 ccccggcggc cagagcgtga acacggccga ttcggccgtg cgcatccggc accttccgac
      781 cgggatcgtc gtgcagtgcc aggacgagcg ctcgcagctc aagaacaagg cgaaggcgct
      841 caaggtgctt cgcgcccggc tgctcgacat ggagatccag gagcaggaga agaagctcgc
      901 cgcctcgcgc aagtcgcagg tctcctccgg ggaccggagc gccaagatcc gcacctacaa
      961 cttcccccag ggacgcgtga ccgaccaccg gatcgggctc acgctctacc ggctgcagga
     1021 cgtgatggag ggggatctga ccgagctgac cgacgccctc ttcgccgccg agcaggccga
     1081 gcgcctcgcc tcctcgggcg aagcgagcgg gccgtcgccc cgcgccggag cgggcgcgtg
     1141 agggcgggaa tccggctgtc gcgcgcggcc aggacggcgc gccgcgcggg caccagccgc
     1201 gccgaggccc tcgcgtcgct cacgcggcgg ttcgagcgcg ccggcatcga ctcgcccgag
     1261 cacgacgccg aagcgatcct gctccgcgcg ctgcaggtct cccgcgccga cctctggacc
     1321 gagcccaacg cgccgctcac cgaggaggag gcggaggacc tgacctcgct cgccgagcgg
     1381 cggagcgcgc gcgagccgct ccagctcctt ttaggaacca cgcccttctg ctcggcgacg
     1441 ctcgatctcg agcgcggcgt cttcctgccg cggccggaga ccgagggcct ggtcgaggcc
     1501 gtcctgcgcg cgctccacgc cgaccggggc cggctcctcg agctcggcgc ggggacgggc
     1561 gcgatcgcga tcgcgatcct gcacgcgctg cccgagtgga ccggcgaagc cgtggaccgc
     1621 tcgccccagg cggtgcgtct ggcgcgaaag aacgcggccc ggaacggcat tgcccggcgc
     1681 ctgcacgtgc tggaggggga ctttcgcgat cccggggcgc tgcgggtccc gggacatgac
     1741 tacgacctgg tcgtatccaa cccgccctac gtccgctcgg gggagatccc ggggctcatg
     1801 cccgaggtcc gggaccacga cccgatcgag gcgctggacg gcggccccga cgggctcgac
     1861 gcgctgcgcg acctggcccg cggaattccg ctctggctgc gcccgggagg gctgctggcg
     1921 ctggagatcg gggccgatca ggccgatgat tcccaggaac tgatgcggcc gcatctcgag
     1981 gagatgcgcg tcctgccgga cctggcaggg aggccgcggg tgctcctggg aacgcggagg
     2041 cgatagcgat ggaagcgaaa gcggcagccg acacgaaagc gacgaagggg acggcgacgg
     2101 gaatggacga gatctgggtt cgcggcggga agcggctgtc gggttccgtg gcggtgagcg
     2161 gctccaagaa cgccacgctt ccggtgctgg cggccgcgct cctggccccc ggcgtctacc
     2221 gcttcaccaa cgtgcccgcg ctcaaggacg tggcgaccat gctcgagatg ctcggacgct
     2281 tcggcgtgaa gagccgccgc accggtccga gcgccgtcga ggtggacacc cgcggccagg
     2341 tgaacgccga agccccctac gagctggtga agaccatgcg cgcttcgatc tacgtgctcg
     2401 gaccgcttct cgcccgctac ggcgaggcgc gcgtctccct gccgggcggc tgcgcctggg
     2461 gcccgcgccc ggtggacctg cacatccgcg gcatggaggc gctgggcgcc gacgtggcga
     2521 tcgagcacgg ctatatcgtg gcgcgctccg agcggctccg cggctcgaac atctccttcg
     2581 acatttccag cgtgggcgcc accgggaacc tcctgatggc cgccgcgacc gcctcgggcg
     2641 aaacggtcat cgagaacgcg gcgtgcgagc cggagatcac ggcgctcgcc gac
//
LOCUS       DASSLZ010000225         6507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_969358, whole genome shotgun sequence.
ACCESSION   DASSLZ010000225 DASSLZ010000000
VERSION     DASSLZ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969358"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VFM00_08515"
     CDS             <1..1434
                     /locus_tag="VFM00_08515"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7498329.1"
                     /translation="QGTAFLTYESGALVGVDWIDRAGSEVHSRSVLMQSSVIDATITM
                     REDGTAIRSSTVLQVAGDVPRDPVTRTFPEGTLYWSDMTPASIEEAVQRARVLGREAV
                     RIPASSLFRESHDEVEVARVDSTDWIVRCHGKRYEVLTDPGGRMLSATLPEYGVTIER
                     REGFGADQYPLWSPNGPPPDGAYRATDVSIRAPQGHVLAGTLTMPPGSGPFPAAVLIT
                     GLSPNNRNEGDPPWMALRDIADALTRSGIAVLRVDDRGVGASTGDRASSTTLDEAADV
                     RTEVAWLRSQPKIRRERVALVGYSEGGLIAPVVAADDPTVAAVVTLAGPGVSGADVAR
                     YQITAAVARDTTIAPTDREAAIRRELNDTLTVREKSYLAIDPLAYARRVRCPALIVQG
                     GNDQHIPVRSAERLAWAMRASGNQDVTVRIVPGVSHSLLPDVNGLSSGWVYLPAFGTS
                     PEILRTVSGWLSVRLTSSGGSSRSANR"
     gene            complement(1480..2931)
                     /gene="malQ"
                     /locus_tag="VFM00_08520"
     CDS             complement(1480..2931)
                     /gene="malQ"
                     /locus_tag="VFM00_08520"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET7498330.1"
                     /translation="MTRRAAGLILHPTSLPGPGPIGTLGSEVDRFLDWAAAAGQSIWQ
                     VLPLGPTGPGNSPYSGISTRAGNPLLIDVAEPGPSGNPGHGRVDYGAAARRHDDLRAS
                     WDSARGDPRAMDELAAFRAAPSQADWLDDWTLFASLRRTHGGAPWTEWEPALRARHPA
                     ALEEARRALRDEIDYQAYLQWLFFRQWDRVRDAARARGIAILGDIPIYVALDSADVWS
                     APEWFRLDEAFRPTEVAGVPPDYFSATGQLWGNPLYRWDRLEADGFAWWIRRLRAELA
                     KFDLVRLDHFRGFESYWAIPARAETAVEGHWESGPGERFFDAARAALGGLPFIAEDLG
                     IVGEEVRALLAATGLPGMRVMQFGFDSADSLHHPARYPEHCVAYTGTHDNDTTRGWFD
                     KAPAWERARALETVGGDGGDIAWRMMLALYRSAAERAIVPVQDVLGLGSEARMNTPSV
                     PEGNWEFRLESGALTPELAERLGTEVHATRRLP"
     gene            3054..5225
                     /gene="glgP"
                     /locus_tag="VFM00_08525"
     CDS             3054..5225
                     /gene="glgP"
                     /locus_tag="VFM00_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET7498331.1"
                     /translation="MPPIPPRLGRLVELAQNLWWSWNPEARELFRRIDTPLWRQTHHN
                     PVRLLQEITPERLSLAAADPENARLYDQVMTAFDQALAGKPSWCSQRYPELSDKLVGF
                     FSAEYGVHQSLPFYAGGLGVLAGDIAKEASDLGIPMVGVGFMYPQGYFRQRVNAEGRQ
                     EEIYEQIDRNKVPVEHVLAPDGARLTIPLSLPDRTVLVEAWRVRLGRTMLYLLDTDLD
                     ENAPWDRELTARLYGGDQTVRLLQEIVLGVGGVRLLRALGLKPAIWHANEGHTAFMML
                     ERAREALAEGRGFDEAVAEVRAASLFTTHTPVAAGHDAFPMDMVEAQLSHVEGFGGPL
                     DPLRGRLLALAEYYGGFNMTVLAMRLGGRVNAVSRRHGEVTRRMWASLWPGTPEAQIP
                     IAAITNGVHVPTWIGSETNRLLRKRLGADWMTRHDDPKLWATASDVLPDEALWQTRCA
                     LKQALLEYMRDRVRSRWSQDRVEPGQTVAFGSMLDPEAFTIGFARRFATYKRADLILH
                     DMERLKRLLTDPRRPVQIVFAGKAHPADENGKQVLQSVYRAARDPEIAGRMAFLEDYD
                     MHAAHWLTQGVDLWLNNPRAPLEASGTSGMKAGLNGIPSASILDGWWVEGYEGGNGWA
                     FGPKPGSDAADDADPGAADARDAESLYRALEEKIVPLFYERNGDGLPHGWLRVVRRAM
                     QTITPAFSARRMMKEYAERMYLPAVTSPEEEQRRPEGVPAP"
     gene            5261..6010
                     /locus_tag="VFM00_08530"
     CDS             5261..6010
                     /locus_tag="VFM00_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498332.1"
                     /translation="MHGIEAADFLTVGLLVLLEAALSADNALVLAVLVLPLPREQQTH
                     ALRYGIIGAFAFRIVATLFAVHLIAWSWIKLIGGLYLLYLPWKHFSHHESSSMPRAAT
                     AILGLSVFWSTVVRVELTDVVFAIDSILVAVGMSRKLWVVISGGLLGILAMRVLIGQV
                     LGLVRRYPAIVDGAYVIVAWVGVKLLVEYAHAMHWIQFEFPRAVAIGVVLALFVLSAI
                     YAVRKERKRPPEKAPAEERPPEKATAEEGAE"
     gene            6007..>6507
                     /locus_tag="VFM00_08535"
     CDS             6007..>6507
                     /locus_tag="VFM00_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498333.1"
                     /translation="MMLMPSGTGPRRAKTRNGIQSGLAALIYGVASLVLTSVFAAPGS
                     AALLPYRDASDLVATTPGTDDGALGATINPAQWGLMERPELSGFWYDRQAPGGFRPPG
                     SWGIAAGSGLGFSMLRTDRHEDAALAGPFVPGKDHVTDYQVGLGFGSPAHANGIALGF
                     SGPGKGA"
BASE COUNT          985 a         2312 c         2245 g          965 t
ORIGIN      
        1 cagggcacgg catttctgac gtacgaatcg ggcgcgctcg tcggcgtcga ctggatcgac
       61 cgcgctggat cggaggtcca ctcccgaagc gtcctcatgc aatccagcgt catcgacgcc
      121 accatcacga tgcgcgagga cgggaccgcg atccgctcct ccaccgtgct gcaggtggcg
      181 ggagacgttc cgcgcgatcc ggtgacccgg accttccccg aggggacgct ctactggtcg
      241 gacatgactc ctgcctccat cgaggaggcg gtccagcggg cgcgcgtcct ggggcgggag
      301 gcggtccgga ttccggccag ctcgctcttc cgcgagagcc acgacgaagt cgaggtggcg
      361 cgcgtcgatt ccaccgactg gatcgtgcgc tgccacggca agcgctacga agtgctgacc
      421 gaccccggcg gccggatgct ctcggcgacg ctccccgaat acggcgtgac catcgagcga
      481 cgcgagggct ttggcgccga tcagtatccg ctctggagcc cgaacggacc gccccccgac
      541 ggcgcctatc gcgcgaccga cgtctcgatc cgggcgccgc aggggcacgt gctcgcggga
      601 actctcacga tgccgcccgg aagcggcccc ttccccgcag cggtgctgat cacgggactc
      661 tcccccaaca atcggaacga gggggatccg ccctggatgg cgctccggga tatcgcggac
      721 gctctgacgc ggagcggcat cgccgttctg cgcgtggacg accgcggcgt gggcgcctcg
      781 acgggagacc gcgcgtcctc caccacgctc gacgaggccg ctgacgtgcg caccgaggtc
      841 gcatggcttc gctcccagcc gaaaatccgc cgcgagcgcg tggccctggt cggctacagc
      901 gagggaggtc tgatcgcgcc ggtggtcgcc gccgatgacc ccacggtcgc ggccgtcgtg
      961 acgctcgccg gcccgggcgt ttccggggcc gacgtcgccc gctaccagat cactgccgcg
     1021 gtggcgcgcg acacgaccat cgcgccgacg gaccgggagg ctgccatccg gcgcgagctg
     1081 aacgacaccc tgaccgtgcg cgagaagagc tatctcgcga tcgaccccct cgcgtacgcg
     1141 cggcgcgtcc gctgccccgc gctcatcgtg cagggcggaa acgaccagca cattccggtg
     1201 cgctcggcgg agcgtctcgc atgggcgatg cgcgcatcgg gaaaccaaga cgtgacggtt
     1261 cggatcgttc cgggcgtgag ccactcgctc ttgcccgacg tgaacggctt gagcagcggc
     1321 tgggtctacc tgcccgcgtt cgggacgtcg cctgaaatcc tgcgcaccgt gagcgggtgg
     1381 ctctcggtcc ggctcacttc ttccggcgga tcgtcacgat cggcgaaccg atgaagagga
     1441 gcatcagggc ggcgaagggg atgtagggaa ggtatcggct cacggcagcc tcctcgtggc
     1501 atggacctcc gtgccaagac gctccgcaag ctccggggtg agcgccccgc tctccaggcg
     1561 aaactcccag tttccctccg gcacggaggg cgtgttcatc cgcgcttcgc tccccagccc
     1621 gagcacgtcc tgtacgggga cgatcgcgcg ctcggccgcg gagcggtaca acgcgagcat
     1681 catccgccac gcgatgtcgc cgccgtcgcc gccgaccgtc tcgagcgccc gggctcgctc
     1741 ccacgccggc gccttgtcga accacccccg cgtcgtgtcg ttgtcgtgcg ttcccgtgta
     1801 ggcgacgcag tgctcgggat agcgggcggg atggtgcagg ctgtcggcgg aatcgaatcc
     1861 gaactgcatc acccgcatgc cggggagccc ggtcgccgcc aatagcgcgc gcacttcctc
     1921 gccgacgatg ccgagatcct cggcgatgaa cgggagtcct ccgagcgcgg cgcgcgccgc
     1981 gtcgaagaag cgctcgcccg gtcccgactc ccaatggccc tcgacagccg tctcggctcg
     2041 ggccggaatc gcccagtacg attcgaagcc ccggaagtga tcgagccgca cgagatcgaa
     2101 cttcgcaagc tccgcgcgca gtctccggat ccaccacgcg aatccgtccg cttccagccg
     2161 gtcccagcga tacagcggat tcccccagag ctgtccggtc gcgctgaagt agtcgggcgg
     2221 tacgccggcg acctccgtgg gacgaaacgc ctcgtccagg cggaaccact ccggcgcgga
     2281 ccagacgtcg gcgctgtcga gcgcgacgta gatcggaatg tccccgagga tcgcgatccc
     2341 cctggcgcgc gcggcgtccc gaacccgatc ccactggcgg aagaagagcc attgaaggta
     2401 cgcttgatag tcgatttcgt cccgcagggc gcggcgggcc tcctcgagcg cggcgggatg
     2461 gcgggcgcgg agcgcgggct cccactcggt ccagggcgct ccgccatggg ttcgtctcag
     2521 ggacgcaaaa agcgtccagt cgtcgagcca gtcggcctgg gacggggccg cacggaacgc
     2581 ggccagctcg tccatcgcgc gcgggtctcc gcgcgcggaa tcccacgagg cacggagatc
     2641 gtcatggcgc cgcgcggcgg cgccataatc gacgcgcccg tgtcccgggt tcccggatgg
     2701 gcccggctcg gccacatcga tcaggagcgg attgccggcc cgcgtggaga tgcccgagta
     2761 gggagagttg ccggggccgg taggtcccag cgggagcacc tgccagatcg actggcccgc
     2821 cgcggcggcc caatcgagaa agcggtccac ctcggagccg agggtcccga tgggccccgg
     2881 accgggcagc gacgtgggat ggagaatcag ccccgccgcg cggcgcgtca tgcgcgcggc
     2941 gagacgcggc gccggaaaag gatcgacgcg gacatcgcga tagtctacaa gggaaccctt
     3001 caccgtgacg agacgacttc gaccatgacg acgcaacgcc gcgcatgacg ccgatgccgc
     3061 ccattccgcc gcggctcggc cgcctggtcg agctggcgca gaacctctgg tggagctgga
     3121 atcccgaggc gcgcgagctg ttccgccgca tcgacacgcc gctctggcgc cagacgcatc
     3181 acaatcctgt ccgccttctc caagagatca cccccgagcg gctctccctg gccgccgccg
     3241 atcccgagaa cgcgcgcctg tacgaccagg tcatgacggc gttcgatcag gcgctcgccg
     3301 gaaagccgtc gtggtgctcg cagcgctacc ccgaactgag cgacaagctg gtcggcttct
     3361 tctccgcgga gtacggcgtg catcagtcgc tccccttcta cgcgggcggc ctcggcgtgc
     3421 tcgcggggga catcgcgaag gaggcgagcg acctcggcat cccgatggtc ggcgtcggct
     3481 tcatgtaccc gcaggggtac ttccggcagc gcgtgaacgc cgaggggcgg caggaggaga
     3541 tctacgagca gatcgaccgg aataaggtcc cggtggagca cgtgctggcg ccggacggcg
     3601 cccgcctcac cattcccctg tcgctgcccg accgcaccgt cctcgtcgaa gcctggcggg
     3661 tgcgtctggg acggacgatg ctctacctgc tcgacaccga cctggacgag aacgccccct
     3721 gggaccgcga gctgacggcg cgcctgtacg gcggcgacca gaccgttcgc ctcttacagg
     3781 agatcgtgct tggcgtcgga ggcgtgcggc tcctccgcgc gctcgggttg aagcccgcca
     3841 tctggcacgc caacgaaggg cacaccgcgt tcatgatgct cgagcgcgcg cgcgaggcgc
     3901 tggccgaggg ccgcggcttc gacgaagcgg tcgccgaagt tcgcgccgcg tcgctcttca
     3961 ccacgcacac gccggtggcc gcggggcatg acgccttccc gatggacatg gtcgaagcgc
     4021 agctctcgca cgtggagggt ttcggcggcc ccctggatcc gctgcgcggg cgcctcctcg
     4081 cgctcgccga gtactatggc ggcttcaaca tgacggtgct cgcgatgcgg ctgggcggac
     4141 gcgtcaacgc ggtgagccgg cggcacggcg aggtgacccg cagaatgtgg gcgtcgctct
     4201 ggcccggcac gcccgaggcg cagatcccga tcgccgcgat cacgaacggc gtgcacgttc
     4261 ccacctggat cgggtcggag acgaaccggc tgctccgaaa gcgcctgggg gcggactgga
     4321 tgacccgtca cgacgatccc aagctctggg cgacggcgtc cgacgtgctc cccgacgagg
     4381 cgctctggca gacacgctgc gcgctgaagc aggcgctgct cgagtacatg cgcgatcgcg
     4441 tgcgttcccg ctggtcgcag gaccgggtcg agccggggca gaccgtggcg ttcggctcga
     4501 tgctcgatcc cgaggcgttc acgatcggct tcgcgcgccg cttcgccacc tacaagcgcg
     4561 cggacctcat cctgcacgac atggagcgcc tgaagcgcct gctcaccgat ccgcgccgtc
     4621 cggtacagat cgttttcgcc ggaaaagcgc atcccgccga cgagaacggc aaacaggtgc
     4681 tgcagtcagt ctaccgggcc gcgcgcgacc cggaaatcgc ggggcgcatg gcgttcctcg
     4741 aggactacga catgcacgcg gcccattggc tgacgcaggg cgtggacctg tggctcaaca
     4801 acccgcgcgc cccgctggag gcgagcggga cgagcggcat gaaggccggg ctgaacggca
     4861 tccccagcgc gagcatcctc gacggctggt gggtcgaggg gtacgagggg gggaacggct
     4921 gggccttcgg tcccaagccc ggctccgatg cggcggacga cgccgatccc ggcgcggccg
     4981 acgcgcgcga cgccgagtcg ctctaccgcg cgctcgagga aaagatcgtg cccctcttct
     5041 acgagcggaa cggcgacggc ctgccccatg gctggctgcg cgtggtgcgc cgcgcgatgc
     5101 agaccatcac gcccgcgttc tcggcccgcc gcatgatgaa ggaatacgcc gagcgaatgt
     5161 acctccccgc cgtcacctcg cccgaggaag agcagcgccg tcccgagggc gtccccgcgc
     5221 cctgacgccc ctccccgttt tggggtagag tcgtccctcc atgcacggta tcgaagcggc
     5281 cgatttcctg accgtcgggc tcctggtcct cctggaagcg gccttgagcg cggacaacgc
     5341 gctggtcctg gccgtcctgg tcctgccgct cccgcgggag cagcagacgc acgcgctgcg
     5401 ctacggcatc atcggggcct tcgcgttccg gatcgtcgcg acgcttttcg cggtgcacct
     5461 gatcgcgtgg tcgtggatca agctgatcgg cggcctctac ctcctctacc tcccctggaa
     5521 gcacttctcc catcacgagt cgagcagcat gccgagggcg gccaccgcca ttctcggact
     5581 ctcggtgttc tggagcaccg tggtgcgggt ggagctcacc gacgtggtgt tcgcgatcga
     5641 ttccatcctg gtcgcggtcg gcatgtcgcg gaagctctgg gtcgtgatct cgggtggcct
     5701 tctcggcatc ctcgcgatgc gcgtcctgat cggacaggtg ctgggcctgg tgcggcgcta
     5761 ccccgccatc gtggacggcg cgtacgtcat cgtggcctgg gtcggcgtga agcttctcgt
     5821 ggaatacgcg cacgcgatgc actggatcca gttcgaattc ccgcgcgcgg tcgcgatcgg
     5881 ggtcgtcctg gcgctgttcg tgctcagcgc catctacgcg gtgcggaagg agcggaagcg
     5941 cccccccgag aaggcgccgg ccgaggagcg ccccccggag aaggccacgg ccgaggaggg
     6001 cgcggagtga tgctcatgcc gtcgggtaca gggccccggc gagccaagac gcggaacggg
     6061 atccaatccg gcctcgccgc tctcatctac ggtgtcgcct ccctcgtcct gacatcggtc
     6121 ttcgcagcgc cggggtcggc cgcgctcctc ccctaccggg acgcctccga cctcgtggcg
     6181 accactccgg gcaccgacga cggagccctg ggcgcgacga tcaaccccgc gcagtggggc
     6241 ctcatggagc gccccgagct ctcgggcttc tggtacgacc gccaggctcc aggcggcttc
     6301 cggcctcccg gctcctgggg catcgcggcc gggagcggcc tgggattctc catgctgcga
     6361 accgaccgcc atgaggacgc ggcgctggcc ggccctttcg tgcctggaaa ggaccacgtc
     6421 accgactacc aggtcggcct ggggttcggg tctccagcgc acgcgaacgg gatcgcgctc
     6481 ggcttctcgg gacccggtaa gggcgcg
//
LOCUS       DASSLZ010000226         3265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_464476, whole genome shotgun sequence.
ACCESSION   DASSLZ010000226 DASSLZ010000000
VERSION     DASSLZ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464476"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFM00_08540"
     CDS             <1..372
                     /locus_tag="VFM00_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7498334.1"
                     /translation="FFTNPVSLQVILSSPPTPPTSDVAEGVSGSPKSRLAQNSPNPFN
                     PSTTIRFALQNRDHVRLSVYSASGRLVSVLENSDLDAGSHSISWHGLDREGHPVGSGV
                     YVYRLVTGSGFEDSKRMVLVR"
     gene            complement(413..1537)
                     /locus_tag="VFM00_08545"
     CDS             complement(413..1537)
                     /locus_tag="VFM00_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7498335.1"
                     /translation="MSAAPMRTGPESAGVATGRGRGVGTRIANQAVPILFAVLCAFGI
                     WVAKITPPFLVGELLVRVGRNTVLVLSLLIPILAGLGLNFGIVIGAMAGQLAAILAVH
                     WGGHGIAGFGAAWLLATPLAVLFGVATGMLFNRAKGREMVTGLIAGFFANGLYQLLAL
                     FALGSLIPFHDPTMVLPQGYGLRNTLDLTPIQYAVDDLIRLRVSLEGVETRVPVVSYL
                     VVLGFCLFTVFFFRTKMGQQFRAMGQDPHVASIAGIPVDRNRLIAIVLSTVFASWGQL
                     LFLQNIGTMNTYSSHEQVGMFAIAALLVSGATVTRATVGQAIIGTILFHTLFIVSPLA
                     GKALAGDAQIGEYFRVFVAYAVITVALVLHAWQVAREARE"
     gene            complement(1534..2619)
                     /locus_tag="VFM00_08550"
     CDS             complement(1534..2619)
                     /locus_tag="VFM00_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7498336.1"
                     /translation="MSAGSSGRTARSGGIGERARGFVAEMGVPRLLIAGFVVALFGTA
                     VATHMDLGSLVTDSIVRVARNGVLVLALLPAIQGGVGLNFGLPLGVICGLIGCVFTLN
                     AGIGGVAGLAAATGIGVALAVPAGLGYGWLLNRTRGQEMMVGTYLGFAVVSAMSIFWL
                     LAPFTNPTLVWAIGGKGLRTTLAMPLDSTKVLDRWLQFTVAGVTIPTGAILVFAALCL
                     LVSWFFRTQMGITIAAARSNPRFARAAGISDERTRVQATVLSTVLAAVGIVIYSQSYG
                     FVQLYTAPLLMAFPAIACILIGGASVTRATVAHVIIGTILFQAILTTALPVTSQVIEG
                     DISETARLIIQNGMILYALTRMGGTRE"
     gene            complement(2616..>3265)
                     /locus_tag="VFM00_08555"
     CDS             complement(2616..>3265)
                     /locus_tag="VFM00_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7498337.1"
                     /translation="KLGIANGVMGLYPATGRVLLRGEPLPLNDPRGALRRGLAFVSED
                     RRGVGLLPDESIELNIALSALEAQGRFLHRFPIKALRPLDQKAMREHALEMIRRLDVR
                     TTGAGQQVRRLSGGNQQKVCLARAMTLRPRVLFVNEPTRGIDVGAKERVLEILAGLNR
                     DDGVTLVVTSSELAELRAICDRIAIVYHGQVAAILPPDAPDARFGLAMAGETAAA"
BASE COUNT          560 a         1145 c         1106 g          454 t
ORIGIN      
        1 ttcttcacga atccggtttc ccttcaggtc attctcagct cgccgcccac gccccccacc
       61 agcgacgtcg cggaaggggt ttcgggatct cccaagtcac ggctcgccca gaactcgccc
      121 aacccgttca atccatcgac cacgatccgc ttcgcacttc agaaccgcga ccatgtccgg
      181 ctctcggtct acagtgcgag cggacgtctc gtctcggtgc tggagaactc ggacctggac
      241 gcggggtctc actcgatctc gtggcatggc ctggaccgag aaggccatcc cgttggctcc
      301 ggagtctacg tttatcggct cgtcacgggg tccgggtttg aggattccaa gcggatggtc
      361 ctcgtgaggt gattcttcga taaagggggg tggccgaggt gaggtgcgaa gactactctc
      421 gcgcctcacg cgccacctgc cacgcgtgca gcaccagcgc caccgtgatc acggcgtagg
      481 ccacgaacac ccggaagtac tcgccgatct gcgcgtcccc ggccagggcc ttcccggcga
      541 gcggcgagac gatgaacagc gtgtggaaga ggatcgtgcc gatgatggcc tggcccacgg
      601 tggcgcgcgt caccgtcgcg cccgaaacca gtaacgcggc gatcgcgaac atgcctacct
      661 gctcgtggga gctgtaggtg ttcatcgttc cgatgttctg aaggaagagg agctgccccc
      721 acgaggcgaa cacggtcgac aggacgatcg cgatcagccg gtttcgatcc acggggattc
      781 cggcgatcga cgcgacgtgc gggtcctggc ccatcgcgcg gaactgctgg cccatcttgg
      841 tgcggaagaa gaagacggtg aacaggcaga acccgagcac gaccaggtac gacaccacgg
      901 ggacgcgcgt ctccaccccc tccagggaca cgcgcaggcg gatcaggtca tccacggcgt
      961 actggatcgg cgtgaggtcg agggtgttcc gcaggccgta tccctgcggc agcaccatcg
     1021 tgggatcgtg gaagggaatc agcgatccga gggcgaacag cgcgagcagc tgatagagcc
     1081 cattcgcgaa gaagccggcg atcagcccgg ttaccatctc ccggcccttc gcgcgattga
     1141 acagcatccc ggtcgcgacc ccgaacagca cggcgagcgg ggtggccagg agccacgcgg
     1201 cgccgaagcc ggcgatgccg tggccgcccc agtgcacggc gaggatcgcg gccagctgcc
     1261 ccgccatcgc gccgatcacg atcccgaagt tcaggcccag ccccgcgagg atcgggatca
     1321 acaaggacag cacgagcacc gtattccgcc ccacgcgcac gagcagctcg ccgacgagga
     1381 aggggggcgt gatcttcgcg acccagatgc cgaaggcgca gagcaccgcg aacaggatcg
     1441 ggaccgcctg gttcgcgatc ctcgtgccga cgccgcgccc gcgccccgtc gcaacccccg
     1501 ccgactccgg gcccgtccgc atcggtgccg cgctcattcg cgcgtcccac ccatccgggt
     1561 cagagcgtag aggatcatcc cgttctggat gatcagccgg gccgtctcgg agatgtcccc
     1621 ctcgatcacc tggctcgtga cggggagggc cgtcgtgagg atcgcctgga agaggatggt
     1681 cccgatgatc acgtgcgcca ccgtggcgcg cgtcaccgag gcgcccccga tcaggatgca
     1741 ggcgatcgcg gggaaggcca tcaggagcgg cgcggtgtag agctgcacga atccgtacga
     1801 ctggctgtag atcacgatcc cgacggcggc cagcacggtg ctgagcacgg tggcctgcac
     1861 ccgcgtgcgc tcgtcggaga tccccgcggc ccgcgcgaac ctcggattgc tgcgcgccgc
     1921 ggcgatcgtg atccccatct gcgtgcggaa gaaccaggac acgagcaggc agagcgccgc
     1981 gaagacgagg atggcgccgg tcgggatcgt gaccccggcg accgtgaact gcagccagcg
     2041 gtcgagcacc ttcgtcgagt cgagcggcat cgccagcgtg gtgcggagcc ccttcccacc
     2101 gatggcccag accagcgtgg gattcgtgaa gggcgcgagc agccagaaga tcgacatggc
     2161 ggagacgacg gcgaatccca ggtaggtgcc gaccatcatc tcctggccgc gcgtgcggtt
     2221 gaggagccat ccgtagccga ggccggccgg aacggccagc gcgacgccga tcccggtcgc
     2281 ggccgccagc ccggcgaccc cgccgatccc ggcgttcagg gtgaacacgc agccgatcag
     2341 gccgcagatc accccgagcg gaagcccgaa gttgagcccg acgcccccct ggatggccgg
     2401 gaggagtgcc agcaccagga cgccgttccg cgcgacgcgc acgatggagt cggtcaccag
     2461 cgagccgagg tccatgtgcg tggcgaccgc ggtgccgaag agggcgacca cgaatcccgc
     2521 gatcaggagc cgcggcacgc ccatctcggc cacgaaccct cgcgcgcgct cgccgatgcc
     2581 gccgctccgc gccgtcctgc cgctcgagcc ggcgctcacg cggccgcggt ctccccggcc
     2641 atcgcgaggc cgaaacgggc gtcgggtgcg tcgggcggca ggatcgcggc cacctggccg
     2701 tgatagacga tcgcgatccg gtcgcagatc gcgcgcagct cggccagctc cgacgaggtg
     2761 accaccagcg tcactccgtc atcccgattc aaccccgcca ggatctcgag cacgcgctcc
     2821 ttggcgccca cgtcgatgcc gcgcgtcggc tcgttcacga acaggacccg cggccgcagc
     2881 gtcatggcgc gcgcgaggca gaccttctgc tgattgcctc cggaaagacg gcgcacctgc
     2941 tgccccgcgc ccgtcgtgcg cacgtcgagc ctccggatca tctccagggc gtgctcgcgc
     3001 atggccttct ggtccagcgg ccggagtgct ttgatcggga agcggtgcag gaaccgcccc
     3061 tgcgcctcca gcgccgagag cgcgatgttc agctcgatcg attcgtcggg caggaggccc
     3121 accccccggc ggtcctccga gacgaaggcg agcccgcggc ggagcgcccc gcgcgggtcg
     3181 ttcaagggga gcggctcgcc gcggaggagg acgcggcctg tggcggggta gaggcccatc
     3241 accccgttgg cgatgcccag cttcc
//
LOCUS       DASSLZ010000227         3781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1709985, whole genome shotgun sequence.
ACCESSION   DASSLZ010000227 DASSLZ010000000
VERSION     DASSLZ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709985"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFM00_08560"
     CDS             complement(<1..531)
                     /locus_tag="VFM00_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498338.1"
                     /translation="MKRAVLACLLCLLALSRPASALVNYDKGSRVILGVQLLQDSDDP
                     SLYYYLPQFPRLAMKEDSTFEFLCVKYVDQAGGTNGGLFHALVEFTLPDPVVKKVEAA
                     LREKVAGAKLMGPVPLMQSVKDGEEGTGSFEVVSAVLADKGENGFTRSVVTSGRAPLM
                     PGSKAVVAALLRQEGAT"
     gene            complement(548..1744)
                     /locus_tag="VFM00_08565"
     CDS             complement(548..1744)
                     /locus_tag="VFM00_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498339.1"
                     /translation="MTRRSYTHLLAVALYVAAATGMPRGVAAAPSWRTQRVMGSLILY
                     QDDARPTRWYYAPAELTVATDADGRPDFHFLDMRYMGSAVSRDQGTFTTRSLVTFRVK
                     LPRASAEELTRAARELAGSGAPVEIRPFPIRRIETALIYASIGSPDTTALPPGRFESS
                     EDGASGPTGESYWTERVYTMGLDDLTAQAFRAALEKGQVYLSLGYAFVGPALASDWGM
                     GELTGSSVLVSELRKSLQAAGSRDSAAADSVRSHVVRAGAIEIALDTKRYPDLMRRVD
                     LNDRAPPGYAALDVYCYDFNNELRPDLAEKEVEIDAEGVAGRRVKLSASFTSTQPDLY
                     SSGIRFPVAVRLDRPYRYRVHEIKNDGSEHAGPWRESASWSRILDVTSPPLTKATGVR
                     SVLGQP"
     gene            complement(1741..>3781)
                     /locus_tag="VFM00_08570"
     CDS             complement(1741..>3781)
                     /locus_tag="VFM00_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498340.1"
                     /translation="VKREEATKIVVTVSYTGFDGGKLWGEVTTSDRKTHRQIKGTSPA
                     PIASTPGEMDLAFELDPNAPDGTLVKTSYLKISVARGDKAAAAYVKAFEMPKTWQNGT
                     RPENVVVTVTPKPAGPTTASLGATPGVPLPPKVIHPSLNRAALLTPTRALTIQPRTAA
                     MSSPTSPAASPAPPPAAQPSGAAVRSINLATTRQMSVSPAMTASPATAKMMAPNTAVE
                     TKTAAASTMIARTDMIAIRNFKFGLPAPDPGSTTAPKGPGDRPVEPLAELRSEDIDLD
                     PSHVLGVFPAFYPDQNPQSGIFYFLPYSYSLRWNEDSGYDLRMIYSATGSGGAGEVAM
                     AARLDAGLGIKERQIATDLIHAYADTHDLTFNALRALPIDSISVSIADDLRRYNIPSD
                     KIAVTGLADFLGQVDVSWITDPVTKENLQQALVEDVGISGRVTLYPSGGKLAPVDVPI
                     QIRLADFVTFGGFRWNRADAWTNRTPYPIKLKYLNALMLDSSSRPIVYSWDLSSARVP
                     PAGRAQWAAASVPGWIDGQAKRIWLEYAVEATCQSCDDAVIRSITGGVSSAGPSMITF
                     HTLTPLADAGVGTGDITVQVRSRYFDPQSRDLQTKSLVLNADGKDFTVGPIYLGSRQP
                     GESVPGDPLFEYSLTVTMGSGQTYPGNSWVSSNDLRQAIGRFQLEQTVGTLPAPK"
BASE COUNT          554 a         1240 c         1380 g          607 t
ORIGIN      
        1 ggtcgccccc tcctggcgaa gcagtgccgc gactaccgcc ttggaccccg gcatgagcgg
       61 cgcgcgtccc gacgtaacga ccgagcgcgt gaaaccgttc tcccccttgt ccgccagcac
      121 cgccgagacc acctcgaagg agccggtccc ttcctcgccg tccttgaccg actgcatcag
      181 gggcaccggc cccatgagct tcgcgccggc caccttctcc cgaagcgccg cctccacctt
      241 cttcacgacc ggatcgggca aagtgaactc gaccagggcg tggaacagcc cgccgttcgt
      301 gccgcccgcc tggtccacgt acttgacgca gaggaactcg aacgtggagt cctccttcat
      361 cgccagacgg gggaactgcg gcaggtagta gtagaggctg gggtcgtcac tatcctgcaa
      421 gagctggacg cccaggatca cgcgcgatcc cttgtcgtag ttcaccagcg cggacgcggg
      481 ccgggacagg gccagaaggc agagcaggca ggcgagtacg gcacgcttca tcagggatac
      541 ctcgcggcta gggttggcca aggaccgatc ggacgcccgt cgccttcgtc aggggcgggc
      601 tcgtgacatc caggatgcgg gaccagctcg cgctctcgcg ccagggaccc gcgtgctcgg
      661 agccgtcgtt cttgatctcg tggacgcgat agcgataggg gcggtccagg cggaccgcga
      721 ccgggaatcg aatgcccgag gagtagaggt cgggttgggt gctggtgaac gaggcggaga
      781 gcttcacgcg cctgcccgcg acgccctcgg cgtcgatctc gacctccttc tcggccaggt
      841 cggggcgcag ctcgttgttg aagtcgtagc agtagacgtc gagggcggcg tagcccggtg
      901 gcgcgcggtc attcaggtcc acccgccgca tcagatcggg gtagcgcttc gtgtcgaggg
      961 cgatctcgat cgcccccgcg cgcacgacgt gagagcgcac cgaatcggcg gcggcgctgt
     1021 ccctggagcc ggcggcctgg agcgacttcc gcaactcgga gaccagcacc gaggagccgg
     1081 tcagctcgcc catcccccag tcggaagcca gagccggtcc gacgaaggcg tagcccaggc
     1141 tcaggtagac ctggcccttc tccagcgcgg cgcggaaggc ctgcgccgtc aggtcgtcca
     1201 gccccatcgt gtagacccgc tcggtccagt agctctcgcc cgtgggtccc gacgctccgt
     1261 cctccgagga ctcgaagcgt cccggtggga gcgcggtcgt gtcggggctt ccgatcgagg
     1321 cgtagatcag cgcggtctcg atgcggcgga tggggaaggg gcggatctcg accggggctc
     1381 cggagcccgc cagctcgcgg gcggcgcgcg tcagctcttc ggcggaagcc cgcgggagct
     1441 tcactcggaa cgtgacgagg cttcgcgtcg tgaaggtgcc ctggtcgcgc gagaccgcgc
     1501 tccccatgta gcgcatgtcg aggaaatgga agtcgggcct gccgtcggcg tcggtcgcga
     1561 ccgtgagctc ggcgggagcg tagtaccagc gggtggggcg cgcgtcgtcc tggtagagga
     1621 tcagcgatcc catgacgcgc tgcgttctcc agctgggcgc cgccgcgacg ccgcgaggca
     1681 tgccggtggc cgccgctacg tacagtgcga ccgcgaggag gtgcgtgtag ctgcgtcgcg
     1741 tcacttgggc gccgggaggg tccccacggt ctgctccagc tggaaccggc cgatcgcctg
     1801 ccggaggtcg ttgctcgata cccaactgtt tcccgggtac gtctggccgc tccccatcgt
     1861 caccgtgagc gagtactcga acaggggatc gccgggcacc gattcgcccg gctgccgcga
     1921 acccagatag atcggcccca cggtgaagtc cttgccgtcg gcgttcagga cgagcgactt
     1981 ggtctggaga tcgcgcgact gcgggtcgaa gtagcgcgac cgcacctgga ccgtgatgtc
     2041 gcccgtgccc acgcccgcgt cggcgagcgg cgtgagcgtg tggaacgtga tcatcgaggg
     2101 gcccgcgctc gacacgccgc cggtgatcga gcggatcacc gcgtcgtcgc agctctggca
     2161 cgtggcctcg accgcgtact ccagccagat ccgcttcgcc tgaccgtcga tccatccggg
     2221 caccgaggcc gccgcccact gggcgcggcc ggccggcgga acccgggcgc tcgagaggtc
     2281 ccacgagtag acgatcggac gcgacgagga atcgagcatc agcgcgttca ggtacttgag
     2341 cttgatggga tagggggtgc ggttggtcca cgcgtcggcg cggttccagc ggaagccgcc
     2401 gaaggtgacg aagtcggcca ggcggatctg gatcggcacg tccaccggcg cgagcttccc
     2461 gccgctggga tagagcgtga cgcggccgct gatcccgacg tcctcgacca gggcctgctg
     2521 gaggttctcc ttcgtgacgg gatcggtgat ccaggagacg tcgacctgtc cgaggaaatc
     2581 ggccaggccg gtgaccgcga tcttgtcgga ggggatgttg tagcgccgca ggtcgtcggc
     2641 gatcgagacc gagatgctgt cgatcgggag cgcgcggagc gcattgaacg tgaggtcgtg
     2701 cgtgtcggca taggcgtgga tcaggtcggt ggcgatctgc cgctccttga tgccgagccc
     2761 ggcgtcgagc cgcgcggcca tcgccacctc gcccgcgccg ccgctgccgg tggccgagta
     2821 gatcatgcgc aggtcgtagc ccgaatcctc gttccagcgg agcgaatacg aatagggcag
     2881 gaagtagaag atccccgatt gcggattctg gtcggggtag aaggcgggga agacgccgag
     2941 cacgtggctc gggtccagat cgatgtcctc ggatcgaagc tcggccagcg gctcgaccgg
     3001 cctgtcgccg ggacccttgg gggccgtggt cgagccgggg tcgggagccg gcaggccgaa
     3061 cttgaagttc cggatcgcga tcatgtcggt gcgggcgatc atcgtcgacg cggccgcggt
     3121 cttcgtctcg acggccgtgt tgggcgccat catcttcgcg gtcgctgggc ttgccgtcat
     3181 cgcggggctc acggacatct ggcgcgtggt cgcgaggttg atcgaacgca cggcggcgcc
     3241 ggagggctgc gccgcgggcg gcggcgcggg cgacgcggcc ggcgaggtcg gcgaggacat
     3301 cgccgccgtc ctcggctgga tcgtgagggc gcgcgtgggc gtgagcagtg cggcgcgatt
     3361 caggctgggg tggatcacct tgggcggcag cggcactccg ggggtggcgc cgaggctggc
     3421 cgtcgtgggg ccggcgggct tcggcgtcac cgtgaccacg acgttctcgg ggcgcgtgcc
     3481 gttctgccag gtcttcggca tctcgaaggc cttcacgtag gccgcggccg ccttgtcgcc
     3541 gcgggccacg ctgattttca gatacgacgt cttcaccagc gtgccgtcgg gcgcgttcgg
     3601 atccagctcg aacgcgaggt ccatctcgcc gggggtgctc gcgatcgggg cgggcgacgt
     3661 gcccttgatc tgccggtggg tcttgcgatc ggaggtcgtc acctcgcccc acagcttgcc
     3721 cccgtcgaag ccggtgtagg agacggtgac gacgatcttc gtcgcctcct cgcgcttcac
     3781 t
//
LOCUS       DASSLZ010000228         6869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1604141, whole genome shotgun sequence.
ACCESSION   DASSLZ010000228 DASSLZ010000000
VERSION     DASSLZ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604141"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFM00_08575"
     CDS             <1..372
                     /locus_tag="VFM00_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498341.1"
                     /translation="LLLLLGVIRGVGGVLLATSGPGLVDSGRIDEGTARLLGGGLLLV
                     GLIAAAASLGCMLRRAWGWTLGLAAVVLFVVDGLINGTMLFGKPGEGGTIVNTVAAVL
                     ILACLVRGRAALRRAPAVAIP"
     gene            complement(403..1686)
                     /locus_tag="VFM00_08580"
     CDS             complement(403..1686)
                     /locus_tag="VFM00_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7498342.1"
                     /translation="MPQPSRRTSLIPFSPIRTMFRLADEMERASGGPVFRLHVGDPDF
                     APPEGVIEATAAALRGGKTHYAPTAGVHELRVALAEKARQKNGIAATPDHIVIAPGST
                     QGLYAAMETIFGVGEEFLLPEIYWPNYIQETLLLGGRPVFYPLGAGYQPDLSALRRLI
                     TPRTRGILINSPSNPTGAVFPEATLRGIWEMARERDLWILSDEAYEDFVFRGAHVSTA
                     SFERDLPEAERRVFSLFTFSKSYAMTGLRLGYLIAPSLYTATLLRKCQEPLVASAAMP
                     TQWGALPAAREPDLGVAAMRDAYRRRRDLALAILKPAGLADYEPDGAFYIMADVSSTG
                     MSGDAFAEALLREERVAVAPGVGFAIQPDVGPDGIPSAMPHASGAPAYPSNPKANQRV
                     RIAFCVSDEELREGLTRMVRFVDRVRANRGVKAGA"
     gene            1920..2492
                     /locus_tag="VFM00_08585"
     CDS             1920..2492
                     /locus_tag="VFM00_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498343.1"
                     /translation="MLASRTAVAAIALSTLVFAGTSSAQGTAAPAKIAPVAAKVAPVS
                     AKPAAPAKAVMPSVSATVKPVSATPAAAAAAKPAKSAAPAGGPRTIVGEVIDPSCWII
                     NGAGGKGHAECAAACAKAGQTLAILEKKTNKVYILAGDKPGEDPNKGVAEYVGQPVLV
                     KGRVYARGGAIGLRVESIEPYNAKLAAETP"
     gene            2528..3073
                     /locus_tag="VFM00_08590"
     CDS             2528..3073
                     /locus_tag="VFM00_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498344.1"
                     /translation="MNRSRRIIVSVLAAAALGTAALGASTPAPAPKGAAKSAPAKQPA
                     AMTGHDHHDMSKMDGAQAKGAPEKGKFTTIVGEVMDPACFLEAGSKSIGPGHFQCAVD
                     CSRSGQTLAIYDRAADRIYFIAGELPGKNPNDPVMRYIHQKVDVSGTVYHRADAWGIV
                     ITKVVPHEGAAAPASAPGAGK"
     gene            3147..5021
                     /locus_tag="VFM00_08595"
     CDS             3147..5021
                     /locus_tag="VFM00_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HET7498345.1"
                     /translation="MNSLVLPLTAIVVFAFAYRYYGAFIAHKVARVDPSRPTPAVTMA
                     DGRDYVRTNKYVLFGHHFAAISASGPLIGPVLAAQFGYLPGALWILIGAALGGAVHDF
                     IILFASVRHRASSLSEIARREIGPVAHGAATAAILFITLLLLAGLGIVVVNALAHSPW
                     GTFTVAMTVPAALLFGLYMYRIRPGRVVEGSLIGVALILAAVLLGPWLVASPYAKWFT
                     LSPRALGVALPVYGFIAATLPVWLLLCPRDYLSTYMKIGTIALLVIGVAIVHPNLRMP
                     AITPFVNGTGPVLPGMKVFPFCFIVIACGAISGFHALIGSGTTPRMVPDEGSIRFIGY
                     GAMLTEGVVAIMALVAATALVPNDYFLINGVPKAVAALGIHPVQLPELTRLVGEETLQ
                     GRTGGAVSLAVGMANILRQIPGMGHLMAYWYHFAIMFEAVFILTALDTGTRVARYLFQ
                     DVLGRVWAPLGSTTNVAAVAGVGALVCAAWGFLTVTGSIETIWPLFGVANQLLAVIAL
                     AVGTTFLLRQRPSRYAWVTLGPLCFLTVSTMSAGVLNTFRYLSPAYVADKGPLVAYLD
                     GILSIVLLLLVATVLLDSVRRWARILALKRSGLVEVPLAVEPAPTPAPSGLAAPEPTL
                     "
     gene            complement(5156..5689)
                     /locus_tag="VFM00_08600"
     CDS             complement(5156..5689)
                     /locus_tag="VFM00_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498346.1"
                     /translation="MKTLRLLALAALAGIALLAIGGCTDTSTRSFLTVVSVNDGNVFY
                     SDLVSDSGTVVADEAKITLGNIPADGSAPLAPGSPYSEIVVTGYSVTYDNGIYPPYSG
                     GLTLRVPSGGTADGSIALSDLGAKASVPTTTATATTARIKVYGFVMANGSNNGDKVEA
                     LASVPVQVANFKDSQLP"
     gene            complement(5922..6533)
                     /locus_tag="VFM00_08605"
     CDS             complement(5922..6533)
                     /locus_tag="VFM00_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004397046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HET7498347.1"
                     /translation="MSKHPVQPLPYANDALKGIGSKTMEIHHDKLYAGYVNKRNEIEE
                     ALQKVDRSKANQIYSEMRGLKAEETFAANGQILHEIYFSIMGGDGQPKGEVVDKIKED
                     FGTFAAWEEDFKASAMCARGWVVLAYDPTDRRLHNFIGDAQNQGGVWGTIPVLNCDTY
                     EHSYFIDYGSDRKAYVEAFMRNINWDEVNRRFTNAASMVMAKK"
     gene            complement(6689..>6869)
                     /locus_tag="VFM00_08610"
     CDS             complement(6689..>6869)
                     /locus_tag="VFM00_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET7498348.1"
                     /translation="WILGEGDGNIAPGVLSYRAPLARNLLGKEVGAEVELTLEEVPRR
                     YRVESIRRRLPGQEN"
BASE COUNT          985 a         2354 c         2387 g         1143 t
ORIGIN      
        1 ctgcttctgc tcctgggggt gattcgcggc gtcggcggcg tcttgctcgc gacgtcgggg
       61 cccggcctcg tggattccgg ccgcatcgac gaggggacgg cgcgcctgct gggcggcggc
      121 ctgctcctgg tcggcctgat cgcggccgcg gcgagcctgg gatgtatgct gcggcgggcg
      181 tggggatgga cgctgggtct cgccgcggtc gtgctcttcg tggtggacgg gctgatcaac
      241 ggcacgatgc tcttcggaaa gcccggcgag ggaggaacga tcgtcaacac cgtcgccgcg
      301 gtgctgatcc tcgcgtgcct cgtgcgcggg cgcgccgcgc tccgtcgggc tccggcggtc
      361 gcgattccct gaacgaagcc gggccgcgat ccggtcgcgg ccttacgctc cggctttgac
      421 cccccgattc gcccgcaccc ggtcgacgaa ccgcaccatg cgcgtcagcc cctcccgcag
      481 ttcttcgtcc gagacgcaga aggcgatccg cacgcgctgg ttcgccttgg gattcgaggg
      541 ataggccggc gcgccgctcg cgtgcggcat ggcgctcgga atcccgtcgg ggcccacgtc
      601 gggctggatc gcgaacccga cgccgggggc gaccgcgacg cgctcttcac gcagcagcgc
      661 ttctgcgaag gcgtccccgc tcatccccgt cgacgacacg tccgccatga tgtagaaggc
      721 gccgtccggc tcgtaatcgg cgagccccgc cggcttcagg atcgcgagcg cgaggtcgcg
      781 ccggcgccga taggcgtccc gcatcgcggc cacgcccagg tccggctcgc gcgcggccgg
      841 gagcgcgccc cactgggtcg gcatcgccgc gctcgcgacc agcggctcct ggcatttcct
      901 caagagcgtc gccgtgtaga gcgaaggggc gatgagatag ccgagacgga gcccggtcat
      961 cgcgtaggat ttcgagaagg tgaagagcga gaacacgcgc cgctcggcct cggggaggtc
     1021 ccgctcgaag gaggctgtcg agacgtgcgc tccgcggaag acgaagtcct cgtacgcctc
     1081 gtcgctcagg atccagagat cccgctcgcg cgccatctcc cagatgccgc ggagggtcgc
     1141 ttcgggaaag accgctccgg tcgggttgct ggggctgttg atcaggatgc cgcgcgtgcg
     1201 cggcgtgatc agccggcgca gggcggacag gtcgggctgg tagccggcgc cgagcggata
     1261 gaagacgggg cgtccgccca ggagcagcgt ctcctggatg tagttgggcc agtagatctc
     1321 gggcagcagg aactcctcgc ccacgccgaa gatcgtctcc atggccgcgt agagcccctg
     1381 ggtcgagccc ggcgcgatca cgatgtggtc cggcgtcgcc gcgatcccgt tcttctggcg
     1441 ggccttctcg gcgagcgcga cccggagctc atgcaccccg gcggtcggcg cgtagtgcgt
     1501 cttgcccccg cgcagggcgg ccgcggtcgc ttcgatcacc ccttccggcg gcgcgaagtc
     1561 gggatcgccg acgtgcaggc ggaaaacggg gcctccgctc gcgcgctcca tctcgtcggc
     1621 caggcggaac atggttcgga tgggggagaa ggggatcagg ctggtgcgcc gcgatggctg
     1681 aggcatgcgt tctcctcggt tttgggtcgg tttcgccccc gcagcatagc atcggcgggg
     1741 cgggcgcgcg cggcgccgcg cccctgccgc ggggtggtac ctccgtccac ttgcgcgtga
     1801 ggggagggcc tgctccggga accgcctggc ttgccgaggg tacggcacgt ttgttacgct
     1861 cgcggcacac cgattccacg gttccagcag tccgcaccgc cctcagagga ggttagggaa
     1921 tgctcgcgtc gagaacggct gtcgcggcga tcgcgctgtc cacgctggtg ttcgccggaa
     1981 cctcatcggc tcagggaacg gccgctccgg ccaagatcgc tccggtggcc gccaaggtgg
     2041 ccccggtgtc cgccaagccc gcggcgccgg ccaaggccgt gatgccgtcg gtctcggcca
     2101 cggtgaagcc ggtctccgcg acccctgccg ccgcggcggc ggccaagccg gcgaagtccg
     2161 cggccccggc gggaggaccg cgcaccatcg tgggcgaggt gatcgatccg tcgtgctgga
     2221 tcatcaacgg cgcgggcgga aagggtcatg ccgagtgcgc cgcggcctgc gccaaggcgg
     2281 ggcagaccct cgcaatcctc gagaagaaga cgaacaaggt ctacatcctg gcgggcgaca
     2341 agcccggcga agatccgaac aagggcgtcg cggagtacgt gggacagccg gtcctcgtga
     2401 aggggcgggt ctacgcccgg ggcggtgcca tcggactccg ggtcgaatcg atcgagccgt
     2461 acaacgcgaa gctcgcggcg gaaacgccat aaggcggaaa cccggggcgc ggaaggagtc
     2521 acatcgcatg aatcgcagca ggcgcatcat cgtttccgtc ctggccgcgg ccgctctcgg
     2581 caccgcggcg ctgggcgctt ccacgcccgc acccgctccg aagggggcgg cgaaatcggc
     2641 tccggccaag cagcccgccg ccatgaccgg gcacgatcac cacgatatgt ccaagatgga
     2701 cggcgcgcag gccaagggcg cgcccgagaa ggggaagttc acgaccatcg tcggcgaggt
     2761 catggacccg gcgtgcttcc tcgaggccgg atccaagtcg atcggcccgg gccatttcca
     2821 gtgcgccgtc gactgctccc gctcggggca gacgctggcc atctacgatc gggcggccga
     2881 ccggatctat ttcatcgccg gcgagcttcc gggcaagaat ccgaacgatc ccgtcatgcg
     2941 ctacatccac cagaaggtgg acgtctccgg cacggtctac caccgcgccg acgcgtgggg
     3001 gatcgtgatc accaaggtcg tgccgcacga gggcgccgcc gctccggcgt cggctccggg
     3061 cgcggggaag tagcggcacc gcccgcggga agtagcgacc cgcccgtcct cgtcgctccg
     3121 agtcacccgg agggccttca aaccgcgtga actcgctggt cctgccgctc acggcgatcg
     3181 tcgtcttcgc gttcgcctac cgctattacg gtgccttcat cgcgcacaag gtcgcgcgcg
     3241 tggatccgtc gcgtcccacg cccgccgtga ccatggcgga cggtcgcgat tacgtccgca
     3301 ccaacaagta cgtcctcttc ggccaccact tcgcggcgat ctcggcctcg ggcccgctca
     3361 tcgggccggt gctggccgcg cagttcggct acctcccggg cgccctctgg atcctgatcg
     3421 gggccgcgct cgggggcgcc gtgcacgatt tcatcatcct cttcgcctcg gtgcgccacc
     3481 gcgcatcctc gctctcggag atcgcgaggc gggagatcgg ccccgtcgcg cacggcgcgg
     3541 ccaccgcggc catcctcttc atcacgctcc tcctgctcgc gggtctcggc atcgtcgtgg
     3601 tgaacgcgct cgcgcactcg ccctggggca ccttcaccgt cgcgatgacg gtcccggccg
     3661 cgctcctctt cggcctctac atgtaccgga tccgccccgg gcgcgtggtg gaggggagcc
     3721 tgatcggcgt ggcgctgatc ctggccgcgg tgctgctcgg gccctggctg gtggcatcgc
     3781 cctacgcgaa gtggttcacg ctctcgccgc gcgcgctggg ggtggcgctg ccggtctacg
     3841 ggttcatcgc cgcgacgctg ccggtgtggc tcctgctctg tcctcgcgac tatctctcga
     3901 cgtacatgaa aatcgggacc atcgcgctgc tcgtgatcgg cgtggcgatc gtccatccca
     3961 acctccgcat gcccgcgatc acgccgttcg tgaacggcac cggaccggtg ctgccgggga
     4021 tgaaggtctt tcccttctgc ttcatcgtga tcgcgtgcgg cgccatcagc ggatttcacg
     4081 cgctgatcgg ctcgggcacg acgccgcgca tggtgcccga cgaagggagc atccggttca
     4141 tcggctacgg cgcgatgctc accgaggggg tggtggcgat catggccctt gtcgcggcga
     4201 ccgcgctcgt gccgaacgac tacttcctga tcaacggcgt gcccaaggcg gtggcggcgc
     4261 tcggcatcca tccggtgcag ctgcccgagc tgacgcgcct ggtgggggag gagacgctgc
     4321 aggggcgcac cggcggcgcg gtgtcgctcg cggtcggcat ggcgaacatc ctgcgccaga
     4381 tcccgggcat ggggcacctg atggcgtact ggtaccactt cgccatcatg ttcgaggcgg
     4441 tgttcatcct gaccgcgctg gacaccggaa cgcgcgtggc gcggtacctc ttccaggacg
     4501 tgctcggccg ggtgtgggcg ccgctgggct cgacgacgaa cgtcgcggca gtggcgggcg
     4561 tcggcgccct ggtctgcgcg gcgtggggct ttctcaccgt gacggggagc atcgagacga
     4621 tctggcccct cttcggcgtg gcgaaccagc tcctggccgt gatcgcgctc gccgtgggga
     4681 cgacgttcct tctgcggcag cgcccctcgc gctatgcgtg ggtcacgctc ggccccctgt
     4741 gcttcctgac cgtcagcacg atgtcggcgg gagtcctgaa cacgttccgc tatctctcac
     4801 ccgcgtacgt cgccgacaaa gggccgctcg tcgcctacct cgacggaatc ctgagcatcg
     4861 tgctcctgct cctcgtcgcg accgtgctcc tcgactccgt gcggcgctgg gcgcggatcc
     4921 tggccctcaa gcggtcgggg ctcgtcgaag tgccgctggc cgtcgagccg gcgcccacgc
     4981 cggcgccgtc cggtctggcc gctcccgaac cgacgctgta agggccaccg ctcaccgagc
     5041 ggacggtggt ggccgccctc gcacgccggg cgccccgctc ggccgccgac ctcaaagaag
     5101 cggcccgagc cgccggagcg gatcgggccg tggggatcgc gcgctgaagg gcgcgtcagg
     5161 gcagctggga gtccttgaag ttcgccacct gtaccgggac gctggccagc gcttccacct
     5221 tgtccccgtt gttcgagccg ttggccatca cgaatccata gaccttgatg cgtgccgtcg
     5281 tggcggtcgc cgtcgtcgtc ggcaccgagg ccttcgcgcc caggtcgctc aacgcgatcg
     5341 agccgtccgc cgtcccgccc gagggaacgc gaagggtgag gccgcccgag tacggaggat
     5401 agatgccgtt gtcgtaggtg accgagtagc cggtcacgac gatctcggag tacgggctgc
     5461 ccggagcgag cggcgccgag ccgtcggccg ggatgttgcc cagcgtgatc ttcgcttcgt
     5521 ccgccacgac cgtgccggaa tccgaaacca ggtcgctgta gaagacgttg ccgtcgttca
     5581 ccgagaccac cgtgaggaag gagcgggtgg acgtgtcggt gcagcctccg atcgcgagca
     5641 gcgcgatccc tgcgagcgcc gcgagcgcga gcaacctgag cgtcttcata tcggtctcct
     5701 tggcctacgc gatccccatc gcgcatgaag cgggtaggaa cggcatacgt aaccccgatg
     5761 ccgggaacgc aaagggcatg ccacaggaag gctcgaaggg cagggttgta agtggccaga
     5821 tttcttgggg ttgccagggt ggaccaactt tgtccgaaga aagcgaaacg ggcgccatgc
     5881 ataacgcatg ggcgcccgcc ggactgcaat gagccgtgga tctatttctt cgccatcacc
     5941 atggacgccg cgttggtgaa acggcggttc acctcgtccc agttgatgtt ccgcatgaac
     6001 gcctcgacgt aggctttccg gtccgagccg tagtcgatga agtaggagtg ctcgtacgtg
     6061 tcgcagttga gcacggggat cgtgccccag acgccgccct ggttctgagc gtcgccgatg
     6121 aagttgtgga gccggcggtc ggtgggatca taggcgagaa cgacccagcc ccgcgcgcac
     6181 atcgccgagg ccttgaagtc ctcttcccac gcggcgaacg ttccgaagtc ttccttgatc
     6241 ttgtcgacga cctcgccctt gggctgcccg tcgccgccca tgatcgagaa atagatttca
     6301 tgaaggatct ggccgttggc ggcgaaggtc tcctcggcct tcaggccacg catctcgctg
     6361 tagatctggt tggccttgct gcggtcgacc ttctggaggg cctcctcgat ctcgttccgc
     6421 ttgttcacgt agccggcgta cagcttgtcg tggtggatct ccatcgtctt cgacccaatc
     6481 cccttgaggg cgtcgttggc gtaggggagg ggttgaacgg gatgcttgga catggggtgt
     6541 tctccttatg tcccgccgcg aagcggcgga atcgatgtcc caccgcgagg cggcggaaat
     6601 gggacgttgg aaatgaggcc gctactatat cagatgcgcc gcggagtcgc ggggaaccgg
     6661 gcggtagccc gcccgcccgg agacaagctc aattctcctg ccccggaagc cgccgccgaa
     6721 tcgattccac ccggtagcgc cgcggcactt cctccagggt cagctccacc tcggccccga
     6781 cctctttccc cagcagattc cgcgcgagtg gcgcccggta ggagagcacc ccgggggcga
     6841 tgtttccgtc cccctcgccc aggatccag
//
LOCUS       DASSLZ010000229         4224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1477570, whole genome shotgun sequence.
ACCESSION   DASSLZ010000229 DASSLZ010000000
VERSION     DASSLZ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477570"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VFM00_08615"
     CDS             <1..829
                     /locus_tag="VFM00_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7498349.1"
                     /translation="PEGWLGKCHALALGEQAAAGDWILFTDGDVTIAPDAARRGVSLA
                     IHERADHVAVAADLEVGGLGEATFLAYFVYAFNLSQRPWDAKDPRSGAHIGIGAFNLV
                     RREAYERAGGHRRLRMELLDDMGLGWIVKQSGGVSVFAGHDGLVRARWQEGLGGLIVG
                     VEKNAFAALRYDVPVTIGAVALQLALSWLPVAGLFAADPRTLAAAILAWSGVVLVYAT
                     TARVVETRTWHALTMPIGATLFGYSILASMVQALRRGGIVWRGTFYSLKELRAGRVR"
     gene            complement(1235..2164)
                     /locus_tag="VFM00_08620"
     CDS             complement(1235..2164)
                     /locus_tag="VFM00_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498350.1"
                     /translation="MSPAAVDGAERLALGTAVAAAGTTTPIAFIVAPSASAGSRGLDE
                     ALRAHRDRLAAGAAYWIRVTPPETSALRRRRVFLGARGRVVIALRGGDGNPYRARDAV
                     VQALRDEAYGPRPLDFELLRKLASGPEPFALIGATGEEAGGGDGGARGSGGGRRSAEE
                     RLRAALFDPRGDVVIPAVPHPDRPRAWLMFETAEAMEAEAIGSHVEALSGGGRAEVVE
                     NFPWDRGNIHHPAIHALIELSREVAEGAEIWPSAPWATPSGVFTRALGVGLAEWGIPL
                     ASGAQVRFPKPEEFEAMAAEAGALLAKPSAEGT"
     gene            2833..3519
                     /locus_tag="VFM00_08625"
     CDS             2833..3519
                     /locus_tag="VFM00_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7498351.1"
                     /translation="MIQAIIFDLDNTLTDFMKMKRAAIDAAVDGMIDAGLKLTHAEIS
                     EKIYNVYDREGIEYQQVFDLFLKEELDRIDYKILTSAIVAYRRARDSYLVLYPHVNLT
                     LMELARRGLRLAVVSDAPRFQAWLRLAHLQLQHVFEPVVAFEDTGERKPSPKPFQRAL
                     EILGIGPDEAIMIGDWPERDVVGAKKAGIRTAFARYGDTFNTRESGADYDLNDIHELV
                     EIVDRLNGAD"
     gene            3527..3886
                     /gene="acpS"
                     /locus_tag="VFM00_08630"
     CDS             3527..3886
                     /gene="acpS"
                     /locus_tag="VFM00_08630"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET7498352.1"
                     /translation="MVSVGVDIVEVERIRRAIGRWGPLFLEKVFTPAEIAYCRGKARE
                     AESFAVRFAAKEAFAKALKVGKASVWQEVEVVRSESPRPALALTGTALALAGSRRVDV
                     SLSHIPTYAVAVVLVED"
     gene            3886..>4224
                     /locus_tag="VFM00_08635"
     CDS             3886..>4224
                     /locus_tag="VFM00_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HET7498353.1"
                     /translation="MRLPPLESRAVTAEQMAALDRATIQGRGIPSLALMERAGREAAK
                     RIAAWWRSEGGTLYGTASRAAGGTQVAGAPARGKAGARKPAARRAPAGGNALILCGRG
                     NNGGDGFVAAR"
BASE COUNT          674 a         1432 c         1457 g          661 t
ORIGIN      
        1 ccccgagggt tggctcggca agtgccacgc gctcgccctg ggcgagcagg ccgccgcggg
       61 ggactggatc ctctttaccg atggcgacgt gacgatcgcg cccgacgcgg cgcggcgcgg
      121 cgtgtcgctc gcgattcacg agcgggccga tcacgtggcg gttgcggccg atctggaggt
      181 cggcgggctg ggggaggcga ccttcctcgc ctacttcgtc tacgccttca acctgagcca
      241 gcggccctgg gacgcgaagg acccgcgctc cggcgctcac attggaatcg gcgctttcaa
      301 cctggtgcgg cgcgaggcct acgagagagc cggagggcac cggcggctcc ggatggagct
      361 tctcgacgac atgggcctgg gttggatcgt gaagcaaagc ggcggggttt cggtgttcgc
      421 gggccacgat gggctggtgc gtgcccgctg gcaggaggga ctgggcgggt tgatcgtggg
      481 ggtggagaag aacgccttcg ccgcgcttcg ctacgacgtg ccggtcacga ttggagccgt
      541 ggcgctgcag ctcgcgctct cctggctgcc cgtcgcgggg ctcttcgcgg ctgatccgcg
      601 cacgctggcc gcggcgatcc tggcctggtc gggcgtcgtt ctcgtctacg cgacgacggc
      661 ccgcgtcgtg gagacgcgca cgtggcacgc gctcacgatg ccgatcggcg ccaccctctt
      721 cggctactcc atcctggcgt ccatggtcca ggcgctgcgc cgtggcggca tcgtctggcg
      781 cggaacgttc tattcgttga aggagctgcg ggcaggtcga gtgcgctagc gcgcctcagc
      841 gcaaaaaaat cccccgcgtg cagggcacgc gggggttgtt catcgcgcgc cacgtggggg
      901 gtcagggcaa acagaatttg ccgacgcgcg actcacatat ggctcgccgc aacgacggcg
      961 aggaagagag ggcgaccagg tgcagatcgg ggagtggtgt cgcgcccctg gtagttccag
     1021 tgtgtcctac cagggaccgg tcgcctcctc ttcgatgtca caccttggca gagcgcgtgc
     1081 cactaccgag cttatcgccg cccctctcta agagacttgg tttcaacaag aaccgaacct
     1141 tccaaaatca acggtctgcg tttgtgtcca aaagatgccg gtggaggtac aacctcgtac
     1201 tgcatcgtac gagtccggcc ctaaatccga gcagttaggt gccttccgca gagggcttcg
     1261 cgagcagagc gcccgcctcc gcggccatgg cctcgaactc ctccggtttg gggaagcgga
     1321 cctgcgcgcc ggaggcgagc ggaatgcccc actcggccag gccgacgccc aacgcgcgcg
     1381 tgaacactcc cgagggggtc gcccagggag ccgagggcca gatttcggct ccctccgcga
     1441 cctcgcgcga gagctcgatc agcgcgtgga tcgcggggtg gtggatgttg ccgcgatccc
     1501 acgggaaatt ctccacgact tccgctcgac ctccgccgga gagcgcctcc acgtggctcc
     1561 cgatcgcctc cgcctccatc gcctccgcgg tttcgaacat gagccaggcg cgaggacggt
     1621 ccgggtgcgg cactgcgggg atcaccacgt ctccgcgcgg gtcgaacagc gcggcccgca
     1681 ggcgttcttc ggccgagcgc cggcccccgc ccgagcctct ggcccccccg tccccacctc
     1741 cggcttcctc gcccgtcgcc ccaatcagag cgaacggctc cgggccggaa gccagctttc
     1801 ggagcagctc aaagtccagc ggacgtgggc cgtaggcttc gtcccggagc gcctggacga
     1861 ccgcgtcccg agcacggtag ggattgccgt cgccgcctcg aagcgcgatc acgacgcgcc
     1921 ctctcgcgcc gaggaagacc ctgcgccgcc gcagcgccga cgtctctggg ggcgtcaccc
     1981 ggatccagta cgccgcaccg gccgccagac gatcccggtg ggcgcgcagg gcctcgtcca
     2041 gcccgcgtga gcccgcgctg gcggaggggg ccacgatgaa cgcgattggc gtcgtggtcc
     2101 cggcggcggc cacggcggtg ccgagagcca gccgttcggc tccatccacg gccgcgggtg
     2161 acacgcccgc cggctccgca tgccatgtct gaaagagaat ggtcgcgggc ggcgcgccgc
     2221 cgcgtgggag cagcagaaag atcggggcaa ctcccgtcga ttcaatgaat tccagcccaa
     2281 gacgatcggc cgcgcctcgc aggtagcgtc ccgcttcccg cgccgtcagc ccggggagcg
     2341 tctcccgggg catcgtcaga aaggactgca attgaatcag ttgggacttc gcatccaggc
     2401 gccttccgga cgtttcgctc gattccaccc ggcccctctc ttcctgttcg aggaaacggc
     2461 atcatagcgg gtccccattc ccttgaaaca atgggatttg tacttgacag ccccgaggcc
     2521 cggtcggagg atcgagtgcg gtcggcgact tggcccccgc cagtgccgtc gggctacaac
     2581 gtggggaggg gaggaatgcg ggagatggaa gtcaactttc ccgaatgccg caagtgttcg
     2641 accggagtcc tgatcccact ctcggactac ggacgggatg gcgccccgat cacctacaag
     2701 gcttgggtct gcaccaatcc cacctgcggc ttcaacgtcc gcatcgacaa tggtgagatc
     2761 agcctcggcc gaaccgtagg tccgtccacc aagtaaacgc gctgcttccc ggctttgcga
     2821 cggtcgaggg ctatgatcca ggccatcatc ttcgatctgg acaacaccct gaccgacttc
     2881 atgaagatga agcgcgccgc gatcgacgcg gccgtggacg ggatgatcga cgccgggctc
     2941 aagctgaccc acgccgagat ctccgagaag atctacaacg tctacgaccg cgaggggatc
     3001 gagtaccagc aggtcttcga tctcttcctc aaggaagagt tggaccggat cgattacaag
     3061 atcctcacca gcgccatcgt ggcctaccgg cgcgcccgcg attcctatct ggtcctctac
     3121 ccgcacgtaa acctgacgct gatggaattg gcgaggcggg gcctccggct ggccgtggtc
     3181 tccgacgcgc cgcgcttcca ggcctggctg cgcctggccc atctccagct ccagcacgtc
     3241 ttcgaaccgg tggtcgcgtt cgaggacacc ggcgagcgga agccgtcgcc caagccgttc
     3301 cagcgcgcgc tcgagatcct cggcatcgga cccgacgagg cgatcatgat cggggactgg
     3361 cccgagcgag acgtggtcgg agccaagaag gcgggaatcc gcaccgcctt cgcccgatac
     3421 ggggatacct tcaatacccg cgaatcgggg gccgactacg acctgaacga catccacgag
     3481 ctggtggaga tcgtggaccg tctgaacgga gccgactagc cgccgcatgg tgagcgtcgg
     3541 ggtggacatc gtggaagtgg agcggatccg ccgggcgatc ggccggtggg gcccgctgtt
     3601 cctggaaaaa gtattcaccc cggccgagat cgcttactgc cgcggaaagg cgcgcgaagc
     3661 cgaatcgttc gccgtgcgct tcgccgcgaa agaggccttc gccaaggcgc tcaaggtggg
     3721 gaaggcgtct gtgtggcagg aggtggaggt ggtccggagc gaatcgccgc gcccggcgct
     3781 ggccctgacc ggcaccgcgc tcgctctcgc cggctcccgc cgggtcgacg tctccctctc
     3841 ccatatcccg acctacgccg tggccgtcgt tctggtggag gactgatgcg ccttccgccg
     3901 ctggaatcgc gcgccgtcac ggccgagcag atggcggccc tggaccgggc cacgatccag
     3961 gggcgcggga tcccctcgct cgcgctgatg gagcgcgcag gacgcgaggc ggcgaagcgg
     4021 atcgcggcat ggtggcggag cgagggcgga acgctctatg ggaccgcttc gcgcgccgcc
     4081 ggcgggacgc aggtcgcagg cgccccggcg cgcggcaagg caggggcgcg gaagcccgcg
     4141 gcgcggcggg cgccggcggg cggcaacgcc ctgatcctct gcgggcgcgg caacaatggc
     4201 ggcgacggct tcgtggcggc ccgg
//
LOCUS       DASSLZ010000230        16514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_202841, whole genome shotgun sequence.
ACCESSION   DASSLZ010000230 DASSLZ010000000
VERSION     DASSLZ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 16514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16514
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202841"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFM00_08640"
     CDS             complement(<1..903)
                     /locus_tag="VFM00_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498354.1"
                     /translation="MAKSGNYTATLSGTIVKQASATTTWFLYPGACQDRAGGTWAPKT
                     AIQADSLDSYTNGTQGGYTAVDLSLKEILWHKVTTAVPSTQRPTNNITGTAALWAGKY
                     DPNWVVKVGYPNLTFQLLYIDTGDHSGATSQYTMTFTQYQSSEQNYDYVYLIGGGNND
                     TDPIGNSRPKLDNLLANGSDGFSRLLCTWTGSILNSTPGATSINGVAGGVAVTGSGSG
                     SPQVVPVSVTMAKADRAVYVLFTADCLFSNEDGLWPFGQGQVWDNISVQNDNAGSATV
                     IWADGTAPVGTDPVTPSSAGQVIQG"
     gene            complement(1497..3629)
                     /gene="glyS"
                     /locus_tag="VFM00_08645"
     CDS             complement(1497..3629)
                     /gene="glyS"
                     /locus_tag="VFM00_08645"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET7498355.1"
                     /translation="MAEPRKEPLLLEIGCEELPASFVGPALEELRALAVAGLREARLG
                     HADARTYGTPRRLALVVEEVAPRQEDRTLEVFGPPAAVAFDANGKPTPAAQGFAKRVG
                     KPVESLERRPGPKGEVLYASVPEPGRASVEVLPELMSGWIHGLHFPKTMHWSGSDRFA
                     RPVRWLVALLGGDVLPVEAFGIRAGRRTQGHRIHASGWADLPRPADYVGTLRERVVMV
                     VPDERRAAIAEGLIRSSGSIGGAPVPDPELLEEVVQLVEWPEPVVGSFDPVYLELPRP
                     VVVTAMRAHQRYFAIEGKDGALLPNFVMIRSGRGEGHDEIRRGTQAVLRARLEDARFY
                     WRNDMKDSLDAKVAALKGIVWHEKLGTVHDRTLRIVQTAEDLARALAPSARGTASRAA
                     FLSKADLASEMVRSGKEFATLQGIIGAEYAAASGEPREVVEAIREHYLPQGPSDPLPS
                     TTAGMLVSLADRLEAVVGGFRAGLEVTGSQDPYGLRRAGNAIVRILLEKKLRLDVLAA
                     ASRTDALFASAGLGTERTADFRAFWALRVESALEEAGITHDTAVAALAVRPGDPLDVL
                     ARAKAVDAVRQSPDFEGLLIGYRRAANLVRTANPEDVPAQDRPMAERAENFGDKVEAD
                     LHLETKMARQAIETYLQGESPDYGAALKHLLGLRPAIDAFFEGVMVMVDDATIRRRRL
                     GLLDAVRQTFLRIADFSALPSAPGQKSV"
     gene            complement(3622..4593)
                     /gene="glyQ"
                     /locus_tag="VFM00_08650"
     CDS             complement(3622..4593)
                     /gene="glyQ"
                     /locus_tag="VFM00_08650"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET7498356.1"
                     /translation="MTEGKKPQTFQEILLRLEQFWSDQGCTLVQPYAGEVGAGTFNPA
                     TFFRVLGPEPWRAAYVEPSRRPKDGRYGENPNRLQNFYQYQVALKPAPADVLDVYLQS
                     LRHIGIDLAKHDVRFVEDDWESPTLGAWGLGWEVWLDGQEITQFTYFQQSGGYDLEPV
                     TAEITYGIERIACYLQGVERIMDLQWSREHTWNDLFLQGEIEWCRFNFEKSDPALLFE
                     LFEKFEREAMRLLNEGLVLPGYDYVVKCSHAFNLLEARGAISVTERVGTIARVRKMAR
                     AAAGAYLKQREELGYPLLRGQGVAASDAPQGAPAGPKKKPLAKAARG"
     gene            complement(4590..5369)
                     /gene="recO"
                     /locus_tag="VFM00_08655"
     CDS             complement(4590..5369)
                     /gene="recO"
                     /locus_tag="VFM00_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET7498357.1"
                     /translation="MAIVQTDALVLRSHRLGETSLIVTLFTRDFGLLRCVAKGARGPK
                     SRFGASLEPGVSIAAVVYRKLTRDLQLLSKADIVAYWPSLWEDPERFARAGSVLEFLE
                     RAAYGEAGDPELIDLAVETLGAMSTAPPPALEAILRAFEVQACRRLGYAPELSSCLEC
                     RAPLAQGGLFHPQKGGLLCGEPCGNDGGAFRLSEKARRTLVALAEHPLESMAGWAMER
                     YPTVEIARAIERFLTSHLERFEGLRAQRVGEAMARYAEGAR"
     gene            complement(5371..6030)
                     /locus_tag="VFM00_08660"
     CDS             complement(5371..6030)
                     /locus_tag="VFM00_08660"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET7498358.1"
                     /translation="MISRLRTHFLTGLLVLAPVAITGYVVWRLFTWVDHLLGTTLRGG
                     YIRPGGVPGLGFLTVIVIIMFAGALAENFVGRRLGKLVDTGLLRIPLLRGLYSTVKDI
                     GTAVLGDRKIAFNQVVLVPFPIPGVYSIGLATSNAPASLSDAAGKPLRGIFLPTPPNP
                     TTGPLLYYPEEMVIPTTMRVDQAIKIVVSAGAVVPLDAIPGVEIPRAEPDARTTVEKG
                     R"
     gene            complement(6094..6537)
                     /gene="ybeY"
                     /locus_tag="VFM00_08665"
     CDS             complement(6094..6537)
                     /gene="ybeY"
                     /locus_tag="VFM00_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET7498359.1"
                     /translation="MSCTERSPGLRSTTLRAIAKETLRLLGRPRASVSIVLTGDEAVR
                     ALNRDFREVDKPTDVLSFPLADPEALRDPETPVFLGEIYISLDTARAQARGARRTFAR
                     EVAHLMVHGVLHLLGYDHHRTGDRKRMWAEERRVMRSVSTLIASL"
     gene            complement(6491..8767)
                     /locus_tag="VFM00_08670"
     CDS             complement(6491..8767)
                     /locus_tag="VFM00_08670"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET7498360.1"
                     /translation="MPDRLQILEPKRKERRSPSSRWAKRFGPKIRRAALFAGFLAFAC
                     FLVPRDPAFEVMTLREGFPAKRDLIAPFQFYLLKDRAVLRHEQTLAARGVAPVYSVDP
                     AAARGTTDLLDSLRAAQGGAGERSGSGWRFQSLGLSAEGARALSGPDGKRILVLARGI
                     AETVYGNGILPDLESAKAATDANGIALERDGMRFEKPWSSLADPTTLRDRAWEEGQRA
                     FPSRPEATQALQELLDVAVRPNLRYEASTTERLRAEARDAVNPYDGLVRKDEKIIGSH
                     ELATADDIRKLESLRAWRSRMHAENNWREGVATVMGRVVLLAFLVIGFLGYVKLHHPA
                     VYRETPHLFLLAILTALVMSVGWVVVNQLRGYEMLIPITILSLTAALLWGNALAFAAT
                     FTASLFTGVVFGLGLPFLTGSALAGIAAIGSATGVRRRRDFYRPMLVVAVAYMVAIGA
                     MGLVDGARWDVLLRRSLWGVVSGFGSVLVVTILLPLLESLFSATTDITLLELADLNRP
                     ILRRLMLEAPGTYHHSLVVGSLAEAGAAAIGGNPLLARVSAYYHDIGKIDKAEYYVEN
                     QSSARNRHEKLSPTMSCLIIESHVREGAEIARKEKLPRAIVDGILEHHGTTLMSFFYQ
                     KALNAPKAPNAEREIEERDFRYPGPKPRSKETAVLMLADSVEAAARSLTEPTPSRIRG
                     VVTRILDARVKDGQLDESPITFEDLANIRESFIPILTALFHARVHYPGSPANEERRRP
                     ADFGVVHGEKSRLKIDHS"
     gene            complement(8794..9798)
                     /locus_tag="VFM00_08675"
     CDS             complement(8794..9798)
                     /locus_tag="VFM00_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7498361.1"
                     /translation="MTNPAKDETTRSVDIEGVDPLSLFGRNDANLRLVERSYPVHVTY
                     REGKLAVKGDPAAVDEVSAILTTLSDLVRRGAVLEEHDVHNFIGLTDAGDREESRTLY
                     ERPVLFSFDKRTVRVKTLGQARYVAALQRHDIVFAIGPAGTGKTYLAVAAAVSALKSR
                     QVERIVLVRPAVEAGESLGFLPGDMKEKVDPYLRPLYDALNDLLSYEKIRRFLDLGVI
                     EVAPLAYMRGRTLNSAFVILDEAQNTTLGQMKMFLTRLGYGSKAVITGDVTQIDLGAS
                     MVSGLVEVQNILKGVHDLSFVYLNERDVVRHHLVREIIRAFEEHEENGREQKRGAEGG
                     "
     gene            complement(9795..10331)
                     /locus_tag="VFM00_08680"
     CDS             complement(9795..10331)
                     /locus_tag="VFM00_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7498362.1"
                     /translation="MKRALPWVVAGLLAGGAAGCGKPVLRVADASLGDYYSDKEFSRL
                     SREQRDDYCNELAMQDSLYQDEIRESEASLAEAKARRAALGGEVDSLLAEAGRREQNI
                     RTLLASRGGADARPGAAPREGTSRWSVRPGDSLWRISSREEIYGNGAEWRRIYESNRG
                     ALSDPDRIFPGQELSIPR"
     gene            complement(10913..12706)
                     /gene="aspS"
                     /locus_tag="VFM00_08685"
     CDS             complement(10913..12706)
                     /gene="aspS"
                     /locus_tag="VFM00_08685"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET7498363.1"
                     /translation="MIEPLAGRRRTHTCGDLRPEDAGKRALLMGWAHRVRDHGGVLFL
                     DLRDRYGITQVVFRPQALNEATMLRAREIGSEWVVLVEGTVLKRPKGSENADLSTGGI
                     ELEADTLAVLNTSKTPPFPIDDAVTTASEDLRLKYRYLDLRRAALRDSIVFRHRLTRS
                     VREYLSDQNFLEIETPLLVKPTPEGARDYVVPARLHPGKFYALPQSPQLYKQILMVAG
                     FDRYFQIARCLRDEDLRADRQPEFTQIDLEMSFPTEDDVFAVVEGMLESVFRRDLARP
                     LPVPFPRITYREAMERYGTDKPDLRIPFEIKDVTAAAGASAFEVFRATAAAAPRCMTA
                     ALAVPGQSGASRKEIDGWEAAAKQMGVAGLAWTRVKGGALDGGVGKHFPGDLATALIH
                     AADAADGSLLLLVGGVRAVAQRALGFLRTQIGNQKFTLDPNDFRFVWVHQFPLFEWDE
                     AHQGWAPAHHIFTMPLDEHLAHLDSDPGRVHGQLYDLAANGNELASGSIRIHRKDIQE
                     RVMRVIGMQPEELAAKFGFLLDSFEYGAPPHGGIALGLDRLVVLFHGGDSIRDAIAFP
                     KTASATSLMDGAPAPVDPADLRELKIRPVLD"
     gene            complement(12703..14013)
                     /gene="hisS"
                     /locus_tag="VFM00_08690"
     CDS             complement(12703..14013)
                     /gene="hisS"
                     /locus_tag="VFM00_08690"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007069143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET7498364.1"
                     /translation="MSFQAPKGTHDILPGESERWNWLEARLRDILERYGYREVRTPMF
                     ESTDLFVRSVGESTDIVRKELYTFEDKGGRSLTLRPEGTAPLVRAYLEHSMGHGQPMA
                     RLYYIGAMFRYERPQAGRFRQFAQVGAELLGPGNPAADGEMIDLFVAVMRGVGLEDPS
                     VLLNSLGDSVCRPIYREKIREYFAGRADELCGDCKERLRTNPLRILDCKVPSCQPVLA
                     GAPRMLDFLCDPCREHFEGVQAALRALSIPFEIAPRLVRGLDYYTRTVFEVHSGSLGA
                     QSAVGGGGRYDLLVEEFGGPSVPALGFSIGLERLLIASGAAAGNAGEPEVVIVSRDPG
                     AALEALALARTLRGVPAPGAPATPQGDAGFREGGLTVVTDVTGKSAGAQMKWASKLGA
                     RYAVFVPADGDGYSLRNMKQGSDEPKQKDAAALRTFLETHAPRP"
     gene            complement(14010..14426)
                     /locus_tag="VFM00_08695"
     CDS             complement(14010..14426)
                     /locus_tag="VFM00_08695"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET7498365.1"
                     /translation="MTGGFARYWLPVIAYVAMIFSLSSIHGSSVPNFFPGVDKLEHLL
                     EYSLFGLLAGRAIRFTLGPTKRRLAAALGTMALGAMVGALDELYQRGVPGRSSDVRDW
                     ITDVGAVAISVLVTQLIHTRPIIRPASNKKVEKTTP"
     gene            complement(14423..15622)
                     /locus_tag="VFM00_08700"
     CDS             complement(14423..15622)
                     /locus_tag="VFM00_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7498366.1"
                     /translation="MSAPDPSRLSFNTRCVHGFGGLDPSTGAVSVPIYQSSTFAFHNA
                     AEGAAIFAGERQGYFYTRLGNPTQAAFECEMASLEGGECALATSSGMAATTTAVFALV
                     EAGDNLIASGTLYGGTHKLFHDLLPRYGVQTRWVDGNDPCAIAKAVDAKTKLFFVETP
                     ANPNLNIIDLRRAAAEAKRHGVPLVVDNTFATPYYQRPLTLGADVVIHSATKYIGGHG
                     DTVAGVIVGTKAFVDRCRNEILRDLGGVISPFNAWLLLRGLKTLPVRMERHSANALQI
                     AQFLDFHPQVERVWYPGLHTHPQRAIAASQMSGFGGMIAFELRGGHDAGVRLMDSVRL
                     ITLAVSLGDCDTLIEHPASMTHSTYTPEERAAFGISDGLVRLSVGIEGVEDLIADLDQ
                     ALETVPV"
     gene            complement(15686..>16514)
                     /locus_tag="VFM00_08705"
     CDS             complement(15686..>16514)
                     /locus_tag="VFM00_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498367.1"
                     /translation="LGPAIQLTTDFFSVASPTANAGRTDIAFTARKNQSDSISIYLLP
                     CAGGDTSMTRLFELRRPSQTFLASPRFAPVGTRLAYTSDSTNGGTGANTAVYFRDGSD
                     LNVGPVAMIDPNVAGSTYLFNPSWGPDKNGDGTPDSLMMAAVDFPGTFFETRHGFYIA
                     ALTPPDGAQTYSQWLDGQFLETPDWSPDGQYVTFVKRNAGTNDRDIWIINRSAADISE
                     AVRVTSGPADDSQPRFSADGNSIFFISNRVDRYGVTGVYGTERRGTNIWSVAHFDRP"
BASE COUNT         2561 a         5599 c         5770 g         2584 t
ORIGIN      
        1 gccctgaatg acctggcccg cgctcgaagg cgtgaccggg tcggtgccca ccggggccgt
       61 gccgtccgcc cagatgaccg tagccgagcc cgcgttgtcg ttctgaacgc tgatgttgtc
      121 ccacacctgg ccctgaccga aaggccacag accgtcctcg ttggagaaga ggcagtccgc
      181 cgtgaacagg acgtacaccg cgcgatcagc cttcgccatg gtgaccgaaa ccggaaccac
      241 ctgcggcgaa cccgagccgg agcccgtgac cgccacgccg ccggccacgc cgttgatcga
      301 cgtcgcgccc ggggtactgt tcaggatcga ccccgtccac gtgcagagca gacgcgagaa
      361 gccgtcggat ccgttcgcga ggaggttgtc cagcttcgga cgggagttgc cgatggggtc
      421 ggtgtcgttg ttgccgccac cgatcaggta cacgtagtcg tagttctgct ccgacgactg
      481 gtactgcgtg aacgtcatcg tgtactgcga ggtggcaccg gagtgatcgc cggtgtcgat
      541 gtacagaagc tggaacgtga ggttcggata gccgaccttg acgacccagt tcggatcgta
      601 cttgcccgcc cacagcgccg ccgtaccggt gatgttgttc gtcggccgct gggtcgaggg
      661 aaccgccgtg gtcaccttgt gccacaggat ttccttgagg gacaggtcga ctgcagtgta
      721 gccgccctgc gttccgttcg tgtagctgtc gaggctgtcg gcctggatcg ccgtcttcgg
      781 cgcccacgtt ccgccggccc ggtcctggca ggcgcccggg tagaggaacc acgtggtggt
      841 cgccgacgcc tgcttcacga tcgttccgct gagcgtcgcc gtgtagttcc cactcttggc
      901 caatgctcca atggctccgg cgcgcccatc aaggggcacc cactgctgag ccttgggcgg
      961 gtcactcgca acccctgcaa gtgcaggcaa tgcccacacc acaagagccg caagccccag
     1021 catggcaatt agggttttcc gcattttgac atcctcctta gcgtttcctg actcggagtc
     1081 ctagttcggg aggactaact ctggaaccga tttgacacgg gttggtacat ccggttgagc
     1141 ttggagcgct gcaaggggga tccgcccgct cgctgggaca gtgctcgagc attttgggcg
     1201 accccgttcg cggcttcgac cgacctcctt tctctcgttt ttttggtggt aagtagttgc
     1261 agaatatttc gatagtagtg tagttgccgc ttggccctct cacccccttc gagctgcctg
     1321 cctatcgctg tatcttctta tttttcagtc cattaaaacg caatgagccg cacccgtagc
     1381 ccacgcgttg tcgcgcatct gatgtaaaat caatcggttc tagcggttgc ggctgttagg
     1441 agacaagtta ttttgcctcc tttaccgacc cagtataggt caaaaacccg ccaaagtcaa
     1501 acggattttt gacctggagc ggatgggagc gccgagaaat cggcgattcg gaggaaggtt
     1561 tgccgcacgg cgtccagcag cccgagtcgg cgtctgcgga ttgtagcatc atccaccatc
     1621 accatgacac cttcgaagaa ggcatcgatg gcgggccgga gtcccagaag gtgcttcagg
     1681 gcggctccgt agtcgggact ctctccctgg aggtaggtct cgatggcctg ccgcgccatc
     1741 ttcgtctcca agtgtaaatc tgcctcgacc ttatcgccga agttctcggc ccgctcggcc
     1801 atcgggcggt cttgcgcggg cacgtcttcg gggttggccg tacgcaccag gttggcggcg
     1861 cggcggtagc cgatcaggag cccctcgaag tcggggctct gccggaccgc gtccacggct
     1921 ttggcccggg ccagcacatc gaggggatcg cccgggcgga cggcgagcgc ggccacggcc
     1981 gtatcgtgcg taattccagc ctcttcgagc gcagactcca cccgcaaggc ccagaatgcg
     2041 cgaaaatccg ccgtcctttc ggtgcccagt cccgcgctcg cgaagagggc gtccgtccgg
     2101 gatgcggcgg ccagcacgtc gaggcggagt ttcttttcca ggaggatcct gacgatcgcg
     2161 ttccctgccc gccggaggcc gtaggggtcc tgggagccgg tgacctcgag ccccgcccgg
     2221 aagcccccga ccaccgcctc caggcggtcg gccaggctga ccagcatccc ggcggtcgtc
     2281 gaggggagcg ggtcggacgg gccctggggc aggtagtgct cccggatcgc ttcgaccacc
     2341 tcccggggct cgccggaggc cgcggcgtac tccgccccga tgatcccctg caaggtcgcg
     2401 aactccttcc cggaccggac catctccgag gccagatcgg ccttggagag gaaggccgcg
     2461 cgcgaggcgg tcccccgggc ggacggcgcg agcgcccgcg ccaggtcttc cgccgtctgg
     2521 acgatgcgga gcgtccggtc gtggaccgtg cccagcttct cgtgccacac gatccccttc
     2581 agggcggcca ccttggcgtc gaggctatcc ttcatatcgt tgcgccagta aaaccgggcg
     2641 tcctcgagac gcgcccggag caccgcctgg gttccgcggc ggatctcgtc gtgcccttcg
     2701 ccgcgcccgc tccggatcat cacgaagttc ggaaggagcg cgccgtcctt cccttcgatc
     2761 gcgaagtagc gctggtgggc gcgcatcgcg gtcaccacga ccgggcgcgg aagctcgagg
     2821 tagaccgggt cgaaggagcc cacgaccggc tcgggccact cgacgagctg gacgacctcc
     2881 tcgagaagct ccgggtcggg aacgggcgcc ccgccgatgc tgccgctgga gcggatcagc
     2941 ccctcggcga tggcggcacg gcgctcgtcc ggcacgacca tgaccacccg ctcgcgcagc
     3001 gtcccgacgt agtcggccgg acgcggcagg tcggcccagc cggacgcgtg aatccggtgg
     3061 ccctgcgtgc ggcggccagc gcgaattccg aaggcctcga ccggaagcac gtcgccgccc
     3121 aggagcgcca ccagccatcg caccggacgg gcgaagcggt cggagccgct ccagtgcatc
     3181 gtcttcggga aatggaggcc gtggatccag ccgctcatga gctcgggaag gacctccacc
     3241 gacgcgcgac ccggctcggg cacgctggcg tacaggacct ctcccttggg gccgggacgc
     3301 cgctcgagcg actcgaccgg ctttcccacc cgtttggcga atccctgcgc ggcgggcgtc
     3361 ggcttcccgt tcgcgtcgaa ggccacggcg gccggcgggc cgaagacctc cagggtgcgg
     3421 tcctcctgcc ggggggccac ctcttcgacg accagcgcga ggcggcgcgg cgtcccgtag
     3481 gtgcgggcat cggcgtggcc gagccgcgcc tcgcgaagcc ccgcgaccgc cagcgcgcga
     3541 agctcttcga gggcagggcc gacgaacgac gcgggcagct cctcgcagcc gatctccagg
     3601 aggagcggtt ccttacgcgg ctcagccacg ggccgcctta gcgaggggct tcttcttcgg
     3661 cccggcaggc gcgccctggg gcgcatcgga cgccgcgacg ccctggccgc gcaggagcgg
     3721 ataccccagc tcctcgcgct gcttcagata ggcgccggcc gccgcgcgcg ccatcttgcg
     3781 cacgcgcgcg atcgttccga cgcgctcggt cacgctgatc gcgccgcgag cctcgagcag
     3841 gttgaacgca tgggagcact tcacgacgta gtcgtagccc ggcagcacca gcccctcgtt
     3901 caagagccgc atcgcttccc gctcgaactt ctcgaacagc tcgaagagga gcgcggggtc
     3961 ggacttctcg aagttgaacc ggcaccactc gatctccccc tggaggaaga gatcgttcca
     4021 ggtgtgctcg cgcgaccact ggaggtccat gatccgttcg accccctgga ggtagcaggc
     4081 gatccgctcg atgccgtagg tgatctcggc cgtgaccggc tcgaggtcgt aaccgcccga
     4141 ctgctggaaa taggtaaact gggtgatctc ctgcccgtcc agccagacct cccagcccag
     4201 accccacgcg cccagcgtgg gcgattccca gtcgtcctcg acgaagcgca cgtcgtgctt
     4261 cgcgaggtcg atgccgatgt ggcgcaggct ctggagatag acgtcgagca cgtcggccgg
     4321 cgccggcttc agcgccacct ggtactggta gaagttctgg agccggttgg gattctcgcc
     4381 gtagcggccg tccttggggc ggcgcgaggg ctcgacgtag gccgccctcc acggctcggg
     4441 gccgagcacg cggaaaaagg tcgcgggatt gaacgtgccc gcgcccacct cgcccgcgta
     4501 cggctggacg agggtgcacc cctggtccga ccagaattgc tccagccgga gcaggatctc
     4561 ctggaacgtc tgcggcttct tcccttcggt caccgggccc cctccgcgta gcgcgccatc
     4621 gcctcgccga cgcgctgcgc gcgcagcccc tcgaagcgct cgagatgcga ggtgaggaac
     4681 cgctcgatcg cgcgggcgat ctccacggtc ggatagcgct ccatggccca gcccgccatc
     4741 gactccagcg gatgttcggc cagggcgacc agggtccgcc gggccttctc ggacaggcgg
     4801 aacgcgccgc cgtcgttccc acacggctcg ccgcagagaa ggccaccctt ctgcggatgg
     4861 aacaggcccc cctgcgcgag cggggcgcgg cactccaggc aggaggagag ctccggcgcg
     4921 tatccgagac ggcggcaggc ctggacctcg aacgcgcgga ggatcgcctc cagcgcgggc
     4981 ggcggcgccg tgctcatcgc gccgagcgtc tcgacagcca ggtcgatcag ctcggggtcc
     5041 cccgcctcgc cgtaggccgc gcgctccagg aactcgagca ccgaccccgc gcgggcgaag
     5101 cgttcggggt cctcccagag cgagggccaa taggcgacga tgtcggcctt ggagaggagc
     5161 tggaggtcgc gcgtgagctt gcgatagacg accgcggcga tcgatacccc cggctccagc
     5221 gaggcgccga agcggctctt gggcccgcga gcccccttgg cgacgcagcg gagcagcccg
     5281 aaatcgcgcg tgaacagggt cacgatcagg ctggtctccc ccaggcggtg ggagcgcagg
     5341 acgagcgcgt cggtctggac gatggccacc ctagcgccct ttctccaccg tggtgcgggc
     5401 gtccggctcc gcacgaggaa tctcgacgcc gggaatcgcg tccagcggca cgaccgcccc
     5461 cgccgagacc acgatcttga tcgcctggtc cacccgcatc gtcgtcggaa tgaccatctc
     5521 ctccgggtaa tagaggagag gccccgtcgt gggattgggc ggcgtgggga ggaagatgcc
     5581 ccgaagcggc ttccccgccg cgtccgacag ggacgccggc gcgttggacg tggccagccc
     5641 gatggagtac acgccgggga tcgggaaggg cacgagcacg acctggttga aggcgatctt
     5701 gcggtcgccc aggacggcgg tgccgatgtc cttgaccgtc gagtagaggc cgcgcaggag
     5761 gggaatccgg agaagccccg tgtcgaccag cttcccgagc cgccgcccca cgaagttctc
     5821 cgccagggcg cccgcgaaca tgatgatcac gatgaccgtg aggaagccca gtcccggcac
     5881 gccgcccggc cggatgtagc cgccgcgcag cgtcgttccg agcaggtggt ccacccaggt
     5941 gaacaggcgc catacgacgt agcccgtgat cgcgaccggc gcgagcacga ggaggcccgt
     6001 gaggaaatgc gtgcgcaagc gggagatcat tggccggcca tcatatcacg ggggctcaaa
     6061 cgcgagcagc gactccaaac gcggccgcct acttcaaagc gatgcgatca acgtcgacac
     6121 gctccgcatc acccgccgtt cttccgccca catccgtttc cggtcccccg tacggtgatg
     6181 gtcgtaccca agaaggtgaa ggaccccatg gaccataagg tgggcgactt cgcgcgcgaa
     6241 cgtgcggcgc gcacccctgg cttgcgctcg cgcggtatcg agcgagatgt agatctcgcc
     6301 aagaaagacg ggggtttccg gatcgcgcag cgcctcggga tcggccagcg ggaaggacag
     6361 cacgtcggtg ggcttgtcca cctcgcggaa gtcgcggttc agcgcgcgga cggcctcgtc
     6421 gccggtcagc acgatcgaga cggacgcgcg gggacgcccc aagagccgga gcgtctcctt
     6481 cgcgatcgcc ctaagagtgg tcgatcttaa gccgggactt ctctccgtgc acgacaccga
     6541 agtcggcggg acggcggcgt tcttcgttcg ccgggctgcc cgggtagtgg acgcgcgcgt
     6601 ggaagagcgc ggtgaggatg gggatgaagc tctcgcggat gttcgcgaga tcttcgaacg
     6661 tgatcgggct ttcatcgagc tggccgtcct tgaccctcgc gtcgaggatc cgggtcacga
     6721 cgccgcggat ccggctgggc gtgggctcgg tgagcgaacg cgccgccgcc tcgacggagt
     6781 cggcgagcat gagcaccgcg gtctccttgg accggggctt ggggccggga tagcggaagt
     6841 cgcgctcctc gatctcgcgc tcggcgttgg gcgccttggg cgcgttcagc gccttctgat
     6901 agaagaagct catcaacgtc gtgccgtggt gctccaggat gccgtccacg atcgcccgcg
     6961 ggagtttctc cttgcgcgcg atctcggcgc cttcgcgcac gtgcgactcg atgatgaggc
     7021 acgacatggt cggcgagagc ttctcgtggc ggtttcgcgc gctcgactgg ttctcgacgt
     7081 agtactccgc cttgtcgatc ttgccgatgt cgtgatagta ggcgctcacg cgcgcgagca
     7141 agggattgcc gccgatcgcc gccgcgcccg cctcagccag gcttcccacc accaggctgt
     7201 gatggtacgt gccgggcgct tccagcatga gccggcgcag gatcggccgg ttcaggtcgg
     7261 ccagctcgag cagcgtgatg tccgtggtcg ccgagaagag gctttcgagg agcggcagga
     7321 ggatggtcac gacgagcacc gagccgaacc ccgagaccac gccccagagc gaccggcgca
     7381 gcaggacgtc ccagcgcgcg ccgtccacca gccccatggc gccgatcgcg accatgtagg
     7441 ccacggcaac gacgagcatc ggccggtaga agtcgcgccg ccggcgcacg ccggtggccg
     7501 atccgatcgc cgcgatgccc gcgagcgcgc tccccgtcag gaacgggagt cccaggccga
     7561 acacgacgcc ggtgaagagc gacgcggtga aggtcgcggc gaacgcgagc gcgttgcccc
     7621 agaggagcgc cgccgtgagc gagaggatgg tgatcgggat cagcatctcg tagccgcgga
     7681 gctggttcac cacgacccac cccacgctca tcaccagcgc ggtcaggatc gccagcagga
     7741 agaggtgggg cgtctcgcgg tagaccgccg ggtggtgcag cttcacgtag cccaggaagc
     7801 cgatcaccag gaacgcgagc agcaccacgc ggcccatcac cgtggcgacg ccctcgcgcc
     7861 agttgttctc cgcgtgcata cggcttcgcc aggcgcgcag cgattccagc ttccggatat
     7921 cgtcggcggt ggcgagctcg tgactgccga tgatcttctc gtccttgcgc acgagcccgt
     7981 cgtacgggtt caccgcgtcg cgcgcttcgg cccggagacg ctcggtcgtg gaggcttcgt
     8041 agcggagatt gggccgcacc gcgacgtcca gcaattcttg aagcgcttgc gtcgcctcgg
     8101 ggcgactggg gaacgcccgc tgcccttcct cccaggcgcg gtcgcgcagc gtcgtcggat
     8161 cggccaggga agaccagggc ttctcgaagc gcatgccgtc gcgctcgagc gcgatgccgt
     8221 tggcgtcggt cgcggccttc gcgctttcca gatcgggcag gatgccgttt ccgtagacgg
     8281 tctcggcgat gccgcgcgcg agcaccagga tccgcttgcc gtccggcccc gagagcgcgc
     8341 gggcgccctc ggccgagagg cccagggact ggaaccgcca gccgcttccg ctccgctcgc
     8401 ccgccccgcc ctgggccgcg cgcagcgaat cgaggaggtc ggtcgtcccc cgcgcggcgg
     8461 cgggatccac ggaatagacc ggggccacgc cccgcgccgc gagcgtctgc tcgtggcgga
     8521 gcacggcgcg gtccttgagg agatagaact ggaacggcgc gatcaggtcc cgcttggcgg
     8581 gaaagccctc gcgcagcgtc atgacctcga aggccgggtc ccgcggcacc aggaagcagg
     8641 cgaacgcgag gaagcccgcg aagagcgcgg cgcgccggat cttgggcccg aagcgcttcg
     8701 cccagcgcga ggagggcgag cgccgctcct tgcgtttcgg ctcgaggatc tgtaatcggt
     8761 ctggcatagc ttgcgcccgg aacggcggcg cgatcagcct ccttcagcgc cgcgcttctg
     8821 ctcgcggccg ttctcttcgt gctcctcgaa ggcgcggatg atctcgcgca ccagatggtg
     8881 gcgcaccacg tcgcgctcgt tcaggtagac gaacgagagg tcgtgcacgc ccttcaggat
     8941 gttctgcact tcgacaagcc ccgagaccat gctcgcgccc aggtcgatct gcgtcacgtc
     9001 gccggtgatg accgccttgg agccgtagcc caggcgcgtc aggaacatct tcatctggcc
     9061 gagcgtcgtg ttctgggctt cgtccaggat gacgaaggcg ctgttcagcg tgcggccccg
     9121 catgtaggcg agcggcgcga cctcgatcac ccccaggtcc aggaaccggc ggatcttctc
     9181 gtaggagagg agatcgttca aggcgtcgta cagcggccgg agataggggt ccaccttctc
     9241 cttcatgtcg ccgggaagga accccaggct ctcccccgct tcgaccgccg gacgcacgag
     9301 cacgatccgc tcgacctgcc gcgacttgag cgcgctcacc gccgccgcga ccgccaggta
     9361 ggtcttgccg gtccccgccg ggccgatcgc gaacacgatg tcgtgccgct ggagggcggc
     9421 gacgtagcgc gcctgcccca gcgtcttcac gcgcacggtg cgcttgtcga aggagaagag
     9481 cacgggccgc tcgtacaggg tgcgcgactc ctcgcggtct ccggcgtcgg tcagtccgat
     9541 gaagttgtgg acgtcgtgct cttcgagcac ggcgccgcgc cgcacgagat cggagagcgt
     9601 ggtcaggatc gcgctcacct cgtccaccgc ggccgggtcc cccttcaccg cgagcttgcc
     9661 ctcgcgatag gtcacgtgca ccggatagct ccgctccacc agccggaggt ttgcgtcgtt
     9721 gcgtccaaaa agggaaagcg ggtcgacgcc ttcgatatcc acggagcgcg tcgtttcgtc
     9781 cttggcggga ttcgtcatcg aggaatgctc agctcctgtc cgggaaagat gcgatcgggg
     9841 tcgctcaggg cgccccggtt cgattcgtag atgcggcgcc attcggcgcc gttgccgtag
     9901 atctcttcgc ggctgctgat gcgccagagg gaatcgcctg ggcgcacgct ccagcggctg
     9961 gttccctccc ggggagccgc ccccggccgc gcatccgccc cgccgcgcga ggcgagcagc
    10021 gtccggatat tctgctcgcg ccgcccggcc tccgccagaa gcgaatccac ctcgccgccg
    10081 agcgcggcgc ggcgcgcctt ggcctccgcc agcgacgctt ccgactcgcg gatctcgtcc
    10141 tggtacagcg agtcctgcat ggcgagctcg ttgcagtagt cgtcgcgctg ctcgcggctc
    10201 agtctggaga attccttgtc cgagtagtag tcgcccagcg aggcgtccgc gacgcgaagc
    10261 accggtttgc cgcaaccggc cgcaccaccg gcgagaaggc ccgccaccac ccaggggagc
    10321 gcgcgcttca tcgacgggtc ttccgggaac ggggcgcggg ccgcgaggcg gcgcgggtca
    10381 gcgaatcttc gagggccttc gctgcttgga tgcgggcagc cgcctcttcg cgtcgggctt
    10441 ccagcgcgga ggcctcctcc gaggcctggc cgacatcccg ttgcagcgct tcggattgct
    10501 gccgatgggc gtccaggatc gaggccggcg tcgggcaggg ggcgttggcg tggccgcatc
    10561 ccgcgacgaa agccccggcc aggaccgtca ggaggccggc caaggcccac gggcgggcgg
    10621 acgaaggccg ggcgggcggc caatcgctgc cggccaaggg ggctccggaa gcgagggccg
    10681 ccgcgtgcgg caggctgtcg ctccggaccg gtttcgctga aagtaaccgc ttgcgtatca
    10741 ataaaataag ttggtcgcga gccctggaag ggggcgcgcg ctgcctccag tcacggtagg
    10801 gacgcgcggg cggcaagtca agggggccgc ccgcccgagg gggtcaatgc aggaggggtg
    10861 ccatacgggg gcgcggccaa ggaggcgggg gggcgccccg gcttaggccc cctcagtcca
    10921 ggacgggccg gatcttcagc tcgcgcaggt ccgccggatc caccggggcc ggggcgccgt
    10981 ccatgagcga cgtggcgctc gccgtcttgg gaaaggcgat cgcgtcgcgg atcgagtcgc
    11041 cgccgtggaa caggaccacc agccggtcca gcccgagcgc gatccccccg tgcggcgggg
    11101 cgccgtactc gaaggaatcg agcaggaagc cgaacttggc ggccagctcc tcgggctgca
    11161 tcccgatcac ccgcatgacg cgctcctgga tgtcctttcg atggattcgg atgctccccg
    11221 aagccagctc gttcccgttc gcggccaggt cgtagagctg gccgtgcacg cggccgggat
    11281 cggagtcgag gtgcgccagg tgctcgtcga gcggcatcgt gaagatgtgg tgcgcgggcg
    11341 cccacccctg gtgcgcctcg tcccattcga agagcgggaa ctggtgcacc catacgaagc
    11401 ggaagtcgtt ggggtcgagg gtgaacttct ggtttccgat ctgcgtgcgc aaaaacccga
    11461 gcgcgcgctg cgccacggcg cggacgccgc cgaccaggag gagaagcgat ccgtccgccg
    11521 cgtccgcggc gtggatcagc gccgtcgcca gatcgcccgg aaagtgcttc cccacgccgc
    11581 cgtccaaggc gccgcccttg acgcgcgtcc aggcgaggcc ggccacgccc atctgctttg
    11641 ccgccgcctc ccagccgtcg atctccttcc gcgacgcgcc cgactgtccc ggcacggcca
    11701 gcgccgccgt catgcaccgc ggcgcggcgg cggcggtcgc gcggaagacc tcgaacgcgc
    11761 tcgcgcccgc ggcggcggtg acgtccttga tctcgaaggg aatccggaga tcgggcttgt
    11821 cggtgccgta gcgctccatg gcctcgcgat aggtgatccg cggaaacgga accgggagcg
    11881 ggcgggccag gtcgcgccgg aagacgctct cgagcatccc ttccaccacc gcgaagacgt
    11941 cgtcctcggt cgggaaggac atctcgaggt cgatctgcgt gaactcgggc tggcggtcgg
    12001 cgcgcaggtc ttcgtcgcgg aggcagcgcg cgatctggaa gtagcggtcg aagcccgcga
    12061 ccatgaggat ctgcttgtag agctgcggcg actgcggaag cgcgtagaac ttcccgggat
    12121 ggagccgcgc gggcacgacg tagtcgcgcg cgccttccgg ggtcggcttc acgaggagcg
    12181 gcgtctcgat ctccaggaag ttctgatccg agaggtactc gcgcacgctc cgggtcagcc
    12241 ggtgacggaa gacgatcgag tcgcggagcg ccgcgcggcg caggtcgagg tagcggtact
    12301 tgagccgcag gtcctcggac gccgtggtga ccgcgtcgtc gatcggaaac ggaggcgtct
    12361 tgctggtgtt caggaccgcc agcgtgtcgg cctcgagctc gatgccgccg gtggaaagat
    12421 cggcgttctc gctccccttc gggcgcttca gcaccgtgcc ctcgaccagc accacccatt
    12481 cgcttccgat ctcgcgcgcg cggagcatcg tggcctcgtt cagcgcctgc gggcggaaca
    12541 ccacctgcgt gatcccgtag cggtcgcgca ggtccaggaa gagcacgccg ccgtggtcgc
    12601 gcacgcggtg cgcccatccc atcaggagcg cgcgcttgcc ggcgtcctcg gggcggaggt
    12661 cgccgcacgt gtgggtgcgc cggcggccgg cgagcggttc gatcatggcc ggggagcgtg
    12721 cgtctcgagg aaggtccgga gcgccgccgc gtccttctgc ttgggctcgt cggatccctg
    12781 cttcatgttc cgcagcgaat atccgtcgcc gtcggccggc acgaagacgg cgtagcgggc
    12841 gccgagcttg gaggcccact tcatctgcgc gcccgcgctc ttgccggtga cgtccgtcac
    12901 gaccgtgagc ccgccttccc gaaagcccgc atcgccctgc ggcgtggccg gagcgcccgg
    12961 cgcgggaacg ccccgcaagg tgcgcgcgag ggccagggcc tcgagcgccg cgccgggatc
    13021 gcgcgagacg atcacgacct ccggttcgcc ggcgttcccg gccgcggcgc cggaggcgat
    13081 gagcagccgc tcgagaccga tcgagaagcc gagcgccgga accgaggggc cgccgaactc
    13141 ctcgacgagg aggtcgtagc gtccgccgcc gcccacggcg ctctgcgcgc cgagcgaccc
    13201 cgagtgcacc tcgaagacgg tccgcgtata gtagtccagt ccgcgcacca gccggggcgc
    13261 gatctcgaag ggaatggaga gcgcgcggag cgcggcctgc accccctcga agtgctcgcg
    13321 gcagggatcg cacaggaaat cgagcatgcg cggcgcgccc gccagcaccg gctggcacga
    13381 gggcaccttg cagtcgagga tccggagcgg gttcgtccgg aggcgctcct tgcagtcgcc
    13441 gcacagctcg tccgcgcgcc ccgcgaagta ctcccggatc ttctcccggt agatggggcg
    13501 gcagacggaa tcgccgagcg agttcaggag cacgctcggg tcctcgagcc cgacgccgcg
    13561 catgaccgcg acgaagaggt cgatcatctc gccgtccgcc gccgggttgc ccgggcccag
    13621 gagctcggcg ccgacctgcg cgaactggcg gaagcgcccg gcctgcgggc gctcgtagcg
    13681 gaacatcgcc ccgatgtagt agagccgcgc catcggctgg ccgtgcccca tcgagtgctc
    13741 gagataggcg cgcacgagtg gcgcggtccc ctcggggcgc agcgtgaggg agcggccccc
    13801 tttgtcctcg aaggtgtaga gctccttccg tacgatgtcg gtcgactccc cgacgctccg
    13861 gacgaagagg tcggtggact cgaacatcgg cgtccgcacc tcgcgatagc cgtagcgctc
    13921 gaggatgtcc cgaaggcgcg cctcgagcca gttccagcgc tcggactcgc cggggaggat
    13981 gtcgtgcgtc cctttgggtg cctgaaagct cacggcgtcg tcttctccac cttcttgttg
    14041 gatgcgggcc ggatgatcgg ccgcgtgtga atgagctggg tgacgagcac cgagatggcg
    14101 acggcgccga cgtcggtgat ccagtcgcgg acgtccgagc tgcggcccgg cacgccccgc
    14161 tggtacagct cgtccagcgc tccgaccatg gcgcccaggg ccatcgtgcc gagcgcggcc
    14221 gccaggcgac gcttcgtcgg gcccagggtg aagcggatgg cacgcccggc caggaggccg
    14281 aacagcgaat attccaacaa gtgctccagc ttgtccacgc ccgggaagaa gttcggcacc
    14341 gacgagccgt gaatggacga gagggaaaag atcatcgcca cgtaggcgat gacggggagc
    14401 cagtagcggg cgaaacctcc ggtcaaaccg gtaccgtctc gagcgcctgg tccaggtcgg
    14461 cgatgaggtc ttcgacgcct tcgatcccca cggagagccg caccagcccg tcggagatgc
    14521 cgaacgcggc ccgctcctcg ggcgtgtagg tcgagtgggt catcgaggcg gggtgctcga
    14581 tcagcgtgtc gcagtccccc aggctcacgg ccagcgtgat caggcggacg gagtccatca
    14641 gcctgacgcc ggcgtcatgg ccgccgcgca gctcgaaggc gatcatcccg ccgaagccgg
    14701 acatctggct ggcggcgatg gcgcgctggg gatgcgtgtg cagccccgga taccacacgc
    14761 gctccacctg ggggtggaag tcgaggaatt gggcgatctg gagcgcgttg gccgagtggc
    14821 gctccatccg cacgggaagc gtcttcagcc cgcgcaggag cagccaggcg ttgaacgggc
    14881 tgatcacgcc gcccaggtcg cgcaggatct cgttccggca gcggtccacg aacgccttcg
    14941 tgccgacgat cacgccggcc acggtatcgc cgtgccctcc gatgtacttg gtcgcgctgt
    15001 ggatcacgac gtcggcgccc agcgtgaggg ggcgctggta atagggggtg gcgaaggtgt
    15061 tgtccaccac gagcgggacg ccgtgccgct tggcctcggc cgccgcccgc cggagatcga
    15121 tgatgttcag gttcggattg gcgggggtct cgacgaagaa gagcttcgtc ttcgcgtcca
    15181 cggccttggc gatcgcgcag gggtcgttgc cgtccaccca gcgggtctgg acgccgtagc
    15241 gcggcaggag gtcgtggaag agcttgtgcg tcccgccgta gagcgtgccg gaggcgatga
    15301 ggttgtcgcc cgcctcgacc agcgcgaaga cggcggtggt ggtggccgcc atgccgctcg
    15361 aggtggcgag cgcgcattcg ccgccttcca gcgaggccat ctcgcactcg aacgcggcct
    15421 gcgtcgggtt gccgaggcgg gtgtagaagt agccctggcg ctcgccggcg aagatggccg
    15481 cgccctcggc cgcgttgtgg aacgcaaagg tgctgctctg atagatgggg accgagacgg
    15541 cgccggtgga ggggtccagc ccgccaaagc catgcacgca acgcgtgttg aagctgaggc
    15601 gagacgggtc cggcgcgctc atcttcctgg ctttgggtgt ggttcggcgg gggcgcgccg
    15661 aagcgcgccg ccgccgcaaa ccggcctacg gccggtcaaa atgagcgacg ctccagatgt
    15721 tcgtgccgcg tcgctcggtt ccatagactc cggtcactcc ataccggtcg acccggttgg
    15781 agatgaagaa gatcgaattc ccgtccgcgc tgaaccgggg ttgcgagtca tccgcgggtc
    15841 ccgaggtcac gcgcaccgcc tcgctgatgt cggccgccga gcggttgatg atccagatgt
    15901 cgcggtcatt ggtgcccgcg ttccgtttca cgaaggtcac gtactgcccg tccggcgacc
    15961 agtccggagt ttccaggaac tgcccatcga gccattgcga ataggtctgc gcgccgtcgg
    16021 gcggcgtgag cgcggcgatg tagaagccgt gccgcgtctc gaagaaggtc ccggggaaat
    16081 cgacggccgc catcatgagg gaatcgggcg ttccgtcgcc gttcttgtcc gggccccagc
    16141 tcggattgaa caggtaggtg ctcccggcca cgttggggtc gatcatggcg accggcccga
    16201 cgttcaggtc gctgccatcg cggaaataga ccgccgtgtt ggctccggtt ccgccgttgg
    16261 tcgagtccga cgtatacgcg agccgggtgc cgaccggggc gaaccgcggg ctcgcgagga
    16321 acgtttggga ggggcggcgc aattcgaaca gacgcgtcat cgacgtgtcc cctccggcgc
    16381 aggggaggag atagatcgag atggaatcgg actggttctt tcgggccgtg aaggcgatgt
    16441 cggtccgacc cgcgttcgcc gtgggagacg ccacgctgaa gaagtccgtc gtcagctgga
    16501 tcgccggacc gagc
//
LOCUS       DASSLZ010000231         6612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_203133, whole genome shotgun sequence.
ACCESSION   DASSLZ010000231 DASSLZ010000000
VERSION     DASSLZ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203133"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VFM00_08710"
     CDS             complement(<1..487)
                     /locus_tag="VFM00_08710"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET7498368.1"
                     /translation="MRRFRFQQGCFVAAGAAALLYALTLSRSVGAGDSGELILAARGL
                     GIAHPPGYALWLLLARLAAAVPAGALAVRVNAVSALATAVATGLFWLVARRAGLRPPA
                     AAVATVLFATAPVLWASAVEAEVYGLAIAGFLALALLAARARAARGTGRDEALFFFAA
                     GL"
     gene            complement(589..969)
                     /locus_tag="VFM00_08715"
     CDS             complement(589..969)
                     /locus_tag="VFM00_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7498369.1"
                     /translation="MFRSKSESGFTLIELVIVIVILGILAAVAIPKYEDMREQARVAT
                     IKGQLGSIRSAIAIQYARNALNGSAVFPVLDGTIFADGRVPKEPVNNVNTVKTTPGVD
                     DTGGWQYTAATGIVKINISTYSSY"
     gene            complement(1137..2354)
                     /locus_tag="VFM00_08720"
     CDS             complement(1137..2354)
                     /locus_tag="VFM00_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7498370.1"
                     /translation="MPVYHYRATSAAGAAQSGVVEAPDLDGAIDTVHRMGLTPIRLEA
                     SAASRPEAARWVLIPKRVATRDLILFVRQLETMLEAGLPILSALEILHQQTTHPVLKA
                     AIDRVRSDVEQGSTLTEALRRHPRCFPAVFTSLIHAGEEGGLLASMLDRVGGLLEYEE
                     ETEQRIRSATFYPTLIVAELCVAFIVLVKFVLPRFASLFRNLDAQLPLPTRILIGASD
                     FFDRWWYLLIMAGIVAAAAAIAWSRTAGGRRRIDGWILRAPVFGPIFQKTIMSRFARV
                     LAALVAAGIPILQALAVARGVAGNRIVEEEVERMRDGVTAGMGLAEAVQGRDVFPPLV
                     VKMLAVGQETGAIDKMLVRVARYFDRDVDYAVKNLSTAIEPVLLAVLGAAVLFTALAV
                     FLPLWNLMNAFRH"
     gene            complement(2358..4067)
                     /locus_tag="VFM00_08725"
     CDS             complement(2358..4067)
                     /locus_tag="VFM00_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET7498371.1"
                     /translation="MRGGRKPLGQSLVEQGRLSPEALTRALEEQRRAGTSLREAILRL
                     SLAPESAILDYYEEQLGIPRMDLSTYVLEPEIVRLLPDKVARQYTVLPLFKIGSTLTV
                     AMADPLDVVALDEVKQSTGLQVEVVVSPEAQIKEAIDRLHPVGSGLDQLAREQALRQV
                     AAEVALRPEEDGPIIRFVNAVVQQAVREGASDLHLEPDENSFRIRLRVDGVLREVSIQ
                     SKALYAPVVSRVKVMAALDIAERRLPQDGRIRMNVSGRDLDLRVSTFPTIHGENLVLR
                     VLDRSASLIGLPELGLAPGPLATVERMVRKPNGIVLVTGPTGSGKTTTLYACIHKINS
                     VERNIVTLEDPVEYHLGSIRQTQVDPDAGLTFARGLRALLRQDPDVIMVGEIRDSETA
                     EIAVRSALTGHLVLSTLHTNDAAGALPRLLDMRIEPFLLSSAVLGVVGQRLVRRVCEK
                     CKRPITPPDDVALALGIDPAQGEFHHGAGCRACSGTGYRGRAGIFEVLEVDEEIQALI
                     HQRAPAEELARAARRAGMASLREDAVRKAASGLTTLEEVLRVTKRDWAQTEDEATAMR
                     AGS"
     gene            complement(4164..5162)
                     /locus_tag="VFM00_08730"
     CDS             complement(4164..5162)
                     /locus_tag="VFM00_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498372.1"
                     /translation="MMRRLPQTSVLVLLAAAALALPASAPAQTLKLASLRVFHSDVVG
                     PWVSYRVRTQSGRTPVREFTQRVAIVSREKVGKGEGYWVELKTVDRAGTRIERGLFAP
                     GPPPSATEARAESEEGSSMDGADSESVADAGTPLRLVRYQMLAPGGRLYEYPIASAMS
                     QRAGGEVSSYELFEFDPTVRPVRRFLGPDTVRVGRYVVPSVIEWTSRAGTDDWPMMED
                     STSTYKLLLTQTYWRNPAISITGFARSLFRVTTKKVPIPQGSTKPVYVGVDTTLAFAP
                     SDTTTGPTSMEPGDGRLLSWTELTLEGVGADAVAEVTQTPEPAPVSDHGPPPGLIR"
     gene            complement(5167..6216)
                     /locus_tag="VFM00_08735"
     CDS             complement(5167..6216)
                     /locus_tag="VFM00_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET7498373.1"
                     /translation="MVQMMDKLGYDVATLGERELSFGQAFLLDAFKKTKIDLVSANVV
                     YADTKKPFLKPYVIRKAGTVRVAFTGLMQKDLITRQHPGDKPLEILDPTETAKKLIPE
                     LRKKADVVVLLSHLGLTDGQKLTIEVPGIDVMVFGHLPGLSKQVVQTQGVINARGGER
                     GQYIPSIHLVVQDGKIASYDGEVVTLDDKIPADETTNKLVDNFQDDLNRRFEAQQKKS
                     VEQQTAQSSQALSADHYLGEKNCRRCHETEYELYSKQQHAQAFATLVKNQRDSTPECL
                     PCHVTGMGQAGGFVSKESTPDLVNVQCENCHGMGTTHGSGNVVGPEVCVTCHNPAQDP
                     SFNFDEKVQKIVHWK"
BASE COUNT         1016 a         2228 c         2365 g         1003 t
ORIGIN      
        1 ccagtcccgc ggcgaagaag aagagcgcct cgtcccggcc cgtgccgcgc gccgctcgag
       61 cgcgtgccgc gaggagcgcc agcgcgagaa agcccgcgat cgccagaccg tagacttcgg
      121 cctcgacggc ggacgcccag aggaccggcg ccgtggcgaa caggaccgtc gcgaccgccg
      181 cggccggagg gcgaaggccc gcccgccgcg ccaccagcca gaagaggccc gtcgcgacgg
      241 ccgtggcgag cgccgagacg gcattcacgc ggacggcgag agcgccggcc ggcacggcgg
      301 cggcgaggcg ggcgaggaga agccagagcg cgtagcccgg gggatgcgcg atcccgaggc
      361 cccgggcggc gaggatcagc tcgccgctgt ctcccgcccc gaccgagcgc gagagcgtga
      421 gggcgtagag gagcgccgcg gcgcctgccg ccacgaagca tccctgctgg aaccggaagc
      481 gccgcatggt tcgcacccgc gaaaagcggg tctccccctg atggtggaac gaggtgggag
      541 cgatcgcccc cacctcgccg actgccgaac cgcccttccc tgggcggatc agtacgatga
      601 gtaggtcgag atgttgatct tcacgatccc cgtggccgcc gtgtactgcc agccgcccgt
      661 gtcgtcgacg cccggcgtcg tcttcaccgt gttcacgttg ttcaccggct ccttcggaac
      721 gcgcccgtca gcgaagatcg tcccgtccag gaccgggaag accgcgctgc cgttcagggc
      781 gttgcgggcg tactggatcg cgatcgccga tcggatcgag ccgagctggc ccttgatcgt
      841 ggccacgcgg gcctgctccc gcatgtcttc gtacttggga atcgccactg cggcgagaat
      901 cccgaggatc acgatgacga tgaccagctc gatcagcgtg aacccgcttt ccgacttgga
      961 ccggaacatg ccgagacctc cttgtgctcg ctcaccaacc cgccctcccc cctgcggggg
     1021 cgggcatgca attccacggg catggatcgg aagacgggcg tgcgatcttg agcgcgttct
     1081 gcaacgcggt ccccggcggg acgggcggcg gcgcaggggc aggaaccggc caacccctag
     1141 tggcggaagg cgttcatcag gttccagagc gggaggaaga cggccagagc ggtgaacagc
     1201 accgcggccc cgagcacggc gaggagaacc ggctcgatgg ccgtggagag attcttgacc
     1261 gcgtagtcga cgtcgcggtc gaaatagcgg gcgacgcgga cgagcatctt gtcgatggca
     1321 cccgtctcct gccccacggc gagcatcttc acgacgagcg gggggaagac gtcgcgcccc
     1381 tgcacggcct cggccagccc catgcccgcc gtgacgccgt cgcgcatccg ctccacctcc
     1441 tcctcaacga tccggttgcc ggccacgccg cgcgccacgg cgagcgcctg gaggatcggg
     1501 atgccggcgg ccaccagcgc cgcgagcacg cgggcgaagc gggacatgat ggtcttctgg
     1561 aagatcggcc cgaacaccgg cgcccggagg atccagccgt cgatgcggcg ccggcccccc
     1621 gcggtgcgcg accaggcgat cgcggccgcc gcggccacga tgccggccat gatcaggagg
     1681 taccaccagc ggtcgaagaa gtcgctcgcg ccgatcagga tccgggtcgg gagcggaagc
     1741 tgcgcgtcca ggttacggaa gagcgacgcg aagcgcggca gcacgaactt gacgaggacg
     1801 atgaaggcga cgcacagctc ggccacgatc agcgtcgggt agaaggtggc ggagcggatc
     1861 cgctgctccg tctcctcctc gtattccagg agcccgccga cgcggtcgag catggaggcg
     1921 aggagccctc cctcctcgcc ggcatggatc aggctcgtga acaccgcggg aaagcagcgc
     1981 ggatggcgcc ggagcgcttc cgtcagcgtg ctcccctgct ccacgtcgga gcggacccgg
     2041 tcgatcgccg ccttgagcac cggatgcgtg gtctgctggt ggaggatctc gagcgcggac
     2101 aggatcggga ggcccgcctc gagcatcgtc tcgagctgcc gcacgaagag gatcaggtcg
     2161 cgcgtggcga cgcgcttggg aatgaggacc caccgcgccg cctcggggcg gctcgccgcc
     2221 gacgcctcca gccggatcgg cgtgagtccc atccggtgca cggtgtcgat cgcgccgtcc
     2281 aggtcggggg cttcgacgac gccggactgc gcggccccgg ccgcgctcgt ggcgcggtag
     2341 tggtagaccg gcacgcgcta cgacccggcg cgcatcgccg tggcctcgtc ctcggtctgc
     2401 gcccagtcgc gcttggtgac ccggaggacc tcctcgaggg tcgtgagccc cgaggcggcc
     2461 ttgcgcaccg cgtcctcgcg gagcgaggcc atcccggcgc gccgcgccgc gcgcgccagc
     2521 tcctccgccg gggcgcgctg atggatcagc gcctggatct cctcgtccac ttcgagcacc
     2581 tcgaagatgc ccgcgcggcc gcgataaccc gttccggagc aggcgcggca gccggcgccg
     2641 tggtggaact ccccctgcgc gggatcgatc ccgagggcca aggccacgtc gtcgggcggc
     2701 gtgatcggcc gcttgcactt ctcgcagacg cggcgcacca ggcgctgccc caccacgccg
     2761 agcacggccg aggagagcag gaacggctcg atccgcatgt cgagcaggcg cggcagcgcg
     2821 cccgcggcgt cgttggtgtg cagcgtggag agaacgagat gcccggtcag cgccgaccgg
     2881 accgcgatct cggcggtctc ggagtcgcgg atctccccga ccatgatcac gtcggggtcc
     2941 tggcgcagca gcgcgcggag gccgcgcgcg aaggtgagcc ccgcgtccgg gtccacctgc
     3001 gtctgccgga tcgagccgag gtgatactcg accggatcct ccagcgtcac gatgttccgc
     3061 tcgaccgagt tgatcttgtg gatgcaggcg tagagcgtcg tggtcttgcc gctccccgtg
     3121 ggtcccgtca cgagcacgat gccgttcggc ttccgcacca tccgctcgac cgtggcgagc
     3181 ggccccggtg ccaggcccag ctcgggcagg ccgatgagcg aggccgagcg gtccagcacg
     3241 cgcagcacca ggttctcgcc atggatcgtg gggaaggtgg agacgcggag gtccaggtcg
     3301 cgcccggaga cgttcatccg gatgcggccg tcctggggca gcctccgctc ggcgatgtcg
     3361 agcgcggcca tcaccttcac gcgcgacacg acgggggcgt agagcgcttt cgactggatc
     3421 gagacctcgc gcagcacgcc gtccacgcgg agccggatgc ggaaggagtt ctcgtcgggc
     3481 tcgaggtgga ggtcggaagc gccctcgcgg acggcctgct gcacgacggc gttgacgaag
     3541 cggatgatcg gtccgtcctc ctcggggcgg agcgcgacct cggcggccac ctggcggagc
     3601 gcctgctcgc gcgcgagctg gtccagcccg gagccgaccg ggtgcagccg gtcgatcgct
     3661 tccttgatct gcgcctcggg agagaccacg acctcgacct ggagtccggt cgactgcttg
     3721 acctcgtcca gggccaccac gtcgagcgga tcggccatcg ccaccgtcag ggtcgagccg
     3781 atcttgaaca gagggagcac ggtgtactgg cgcgcgacct tgtcggggag gagccggacg
     3841 atctcgggct cgaggacgta ggtgctcagg tccatgcggg gaatgccgag ctgctcctcg
     3901 tagtagtcga ggatcgcgga ctcgggggcc aggctcagtc ggagaatcgc ctcccggagc
     3961 gacgtccccg cgcgacgctg ctcttccagc gcgcgcgtga gcgcctcggg cgacaggcgc
     4021 ccctgctcga ccaggctctg accgagcggt tttcgacctc cacgcatggc ggatcccccg
     4081 aacggagtcg cgcgggcgcg tccatgcccc gggcggttcg tctcagagat cggcgcgaaa
     4141 ggggcggatc tggagcggtt tagctaccgg atgagccctg ggggcggtcc gtggtcggag
     4201 accggcgcgg gctccggggt ctgcgtcacc tcggcgacgg cgtcggcgcc gacgccttcc
     4261 agggtcagct cggtccagga gagcaggcgg ccatcccccg gctccatcga ggtggggccg
     4321 gtcgtcgtgt cggagggcgc gaacgccagc gtcgtgtcga ccccgacgta gaccggcttc
     4381 gtggagccct gcgggatggg gactttcttg gtcgtgacgc ggaagaggga tcgggcgaat
     4441 ccggtgatgg agatcgccgg attgcgccag taggtctgcg tcagaaggag cttataggtg
     4501 gaggtgctgt cctccatcat cggccagtcg tcggtgcccg cgcgcgaggt ccactcgatc
     4561 accgagggaa ccacgtagcg ccccacgcga accgtgtcgg ggcccaggaa gcggcgcacc
     4621 gggcgcacgg tgggatcgaa ctcgaacagc tcgtagctgg agacctcgcc gcccgcgcgc
     4681 tgcgacatgg cggaggcgat cgggtactcg tacagcctcc cgcccggtgc gagcatctgg
     4741 tagcggacca gacggagcgg cgttcccgcg tcggccacgc tctcggagtc ggcgccgtcc
     4801 atggaactcc cctcctcgga ttccgcccgc gcctcggtgg cgctgggcgg cgggcccggg
     4861 gcgaagagcc cgcgctcgat ccgcgtgccc gcccggtcga ccgttttcag ctccacccag
     4921 tagccctccc ccttccccac cttctcgcgg gagacgatgg cgacgcgctg cgtgaactcg
     4981 cggacgggag tgcggccgct ctgggtgcgg acgcggtagg agacccaggg accgacgacg
     5041 tcggaatgga agacgcgaag ggaggccagc ttcagggtct gcgccggggc gctcgccggg
     5101 agggcgagcg ccgcggccgc gaggagaacg agaaccgatg tctgcgggag tcgtcgcatc
     5161 atcggcctac ttccagtgga cgatcttctg aaccttttcg tcgaagttga acgacggatc
     5221 ctgtgccggg ttgtggcagg tgacgcagac ctccggtccc acgacgttcc cggagccgtg
     5281 cgtggtgccc atgccgtggc agttctcgca ctgcacgttc acgaggtcgg gcgtgctttc
     5341 cttggagacg aagccgccgg cctgtcccat gccggtgacg tggcagggga ggcactccgg
     5401 cgtggagtcg cgctggttct tgaccagggt cgcgaacgcc tgcgcgtgct gctgcttcga
     5461 gtacagctcg tactccgtct cgtggcagcg gcggcagttc ttctcgccca ggtagtggtc
     5521 ggcgctcagg gcctgcgagc tctgcgcggt ctgctgctcc accgacttct tctgctgggc
     5581 ctcgaaacgg cggttcaggt cgtcctggaa gttgtccacc agcttgttgg tcgtctcgtc
     5641 cgccggaatc ttgtcgtcca gcgtcacgac ctcgccgtca taggaggcga tcttgccgtc
     5701 ctgcacgacg agatggatcg acgggatgta ctggccccgc tccccgccgc gcgcgttgat
     5761 gacgccctgg gtctgcacca cctgcttgga gaggccgggg agatggccga agaccatgac
     5821 gtcgatgccc gggacctcga tcgtgagctt ctgtccgtcg gtgagcccga ggtgggagag
     5881 gagcacgacc acgtcggcct tcttccggag ctcggggatc agcttcttcg ccgtctcggt
     5941 cggatcgagg atctcgagcg gcttgtcgcc ggggtgctgc cgggtgatca ggtccttctg
     6001 catgaggccg gtgaacgcca cgcgcaccgt ccccgctttc cggatgacgt agggcttcag
     6061 gaagggcttc ttggtgtccg cgtagaccac gttcgcggag accaggtcga tcttggtctt
     6121 cttgaaggcg tccagcagga acgcctggcc gaacgaaagc tcccgctcgc cgagcgtcgc
     6181 cacgtcgtag cccagcttgt ccatcatctg gaccatgaac gcggacttca gcgaatcctg
     6241 ctcgccggcg acgccgaaga agtctccggt atcgaccgtg aagtggttcg ggaacttgag
     6301 gctgcctacc ttgagttggc tcgccctccg ggcgaggccg ccgagcgggt gcgcgtgtca
     6361 gccacagggc gcgagctcgt tgaacacgtc gttcgtgctc tggatcagga tgtccgtctg
     6421 ggagccctta ccctggagcg acgaggcggc aaacagaagg ccggcaatga gaaacaggag
     6481 atagcggtag cgaagcagcg cggatcacct ccctcgaatc gatgttcgga aagaacgtct
     6541 gaaaactata ccccgtactc cgtacgtggt tcaacacaag gcgccttttt gtggggggat
     6601 gggctgcttc ca
//
LOCUS       DASSLZ010000232         6137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_973139, whole genome shotgun sequence.
ACCESSION   DASSLZ010000232 DASSLZ010000000
VERSION     DASSLZ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973139"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..725)
                     /locus_tag="VFM00_08740"
     CDS             complement(3..725)
                     /locus_tag="VFM00_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7498374.1"
                     /translation="MRQGLFVTIVAVVALGISIAIYNFLPEYIKQGGPLVALLIMLSI
                     MSVTFTLERLFTLMRAKGKEPAPTFLRKVRQKIDALDVDGAAELCSKQRGSLANVLRA
                     GLDRYAIVRDAPLDRQAKIQEVQKAMEEANMLEVPLLERNLIALATIASIATMVGLLG
                     TVIGMIRSFAALGHTGSVDAVKLAVGISEALINTAGGLFVAIFSIVLYNVFVTMIDNF
                     NYMMDEATLEVIEVLSIKEASK"
     gene            complement(827..1165)
                     /locus_tag="VFM00_08745"
     CDS             complement(827..1165)
                     /locus_tag="VFM00_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7498375.1"
                     /translation="MSLDVRTSGDVTILAPKGMLLGGKETDELSGKIKELSEAGNKKL
                     LINLGQTTFMNSVSLGVLIAGHSSYAKRGAQLKLCAVDKKIQNIFVVTRLSLVFETYE
                     TEEEAIKSFK"
     gene            complement(1203..3071)
                     /locus_tag="VFM00_08750"
     CDS             complement(1203..3071)
                     /locus_tag="VFM00_08750"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7498376.1"
                     /translation="MNSVPRRIGLVLLVLGLSAFAAAAWALNGSDLIKQGVTALKDGK
                     AQQALDIFSRAQRLEPNSPKPHYYIASALERMNNADSARAEYETAIRMDPKYTEALTG
                     LGNLLRKQGKIPEGTEKLEAAVKFDPKDPAALYSLGTAYLKDKKYDEAEKVFRKGTLL
                     KTGRAQFLAGTALALEGKGQMKEAEEIFIRARETDPNNLRVRLELGGFYERKKIPFLA
                     VPEYKKAKELDPKNAEYHYLYGRASVGMNEFNEGLRAFVEANNVDSTYAPAYLESGRL
                     FYRAKRYPEAVEKFSKYVELMPDDAQGSLELGRALSYSRDPAERAMAIGNLEKALEKR
                     PKDKEILGTLCKLYSEQGDEGKDNAMKACTQYADVADTLAPDERIRIGTIFASLGDTT
                     RAVPMLTSAAQEDTSLARDSYFQLGFLYFKNQDYAGSVPYFEKTLAVDPEFVPALLNL
                     GLAKMQLKENPEAVGYLRRAVALRPNDRANAVRAHVWIGQILMQMPADSLPSALQSFQ
                     EAAAADSVNGDAIRGAGLSYLLMDNCPNAIQWLSRAAAVDPEHVQGHIWLAQGYLKCK
                     NIPLAKVEFNKAIDLDPTNRPASDGLNLIRKYEASQQQVQQKRAAQSSPSSSGSKP"
     gene            complement(3068..3997)
                     /locus_tag="VFM00_08755"
     CDS             complement(3068..3997)
                     /locus_tag="VFM00_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7498377.1"
                     /translation="MRGVDRALRVRALAVALVLAALVSGCGPRSSPPRDEPPSTPIVL
                     AGAEYVGPLLRAEILAFRERYPASDSIRVVTNGSAEGMEQLVNGDVSMSVLLRDLTDP
                     EAEAAVRREGLQTFPFAWDAVAVIVNPGSPLEQLSRSELGWIYSGSTSSWADLGWKAG
                     GPIIALTSGTRLGMYAYVEQTLLNGGTYAKTVYAPPTEEEAVDVVATRRNAIACVSRP
                     YAEAAGDRVRVLRIAQAKGLPYIPLTRETVLQRTYPLLRSIALATPAKVRETASELIT
                     FISGVDGQAIVARYGYAPATVPIRIVRTAEEAE"
     gene            complement(4208..>6137)
                     /locus_tag="VFM00_08760"
     CDS             complement(4208..>6137)
                     /locus_tag="VFM00_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HET7498378.1"
                     /translation="DTLGLSLEGRPLVAIEISDNPGIDEGEPEVLVDGCLHARELMSV
                     EVPLFAMRRLLDGYGTDPFLTQVVDTRRIWILPVANPDGYVYVEQHSGGQSTGWWRKN
                     RRPNADGSFGVDLNRNYGFEWAYDDVGSSPTTTAETYRGTAAFSEPETQVLRDFIGSR
                     RFTVSLSFHSYGDLVLYPWGYDRLDVPDNAVFSALGDSIGSYNGYRVGNPKSNAIYLT
                     NGGIDDWLYGDASDKPVVYGYTIELNTEEEGGFDPPDALIGPTCEANWGGVLTVLRYA
                     DEPKRVLGPARPESPAVVVGPGGTAQMRWSYPVPDPANPAARHDVRRIAQVLRGLDDA
                     EGGTADWDTLRFAWSTARKADGSHSYGSGSSDNRESILSSRASFDVGMGDSLVAWAWW
                     DLESGFDYWYAQASVDGGGTWHSLAGTHTTNLNPSGQNEGFGVTGSSGGVFVRTAFSL
                     LPYVGKQALVRFRCVTDAANHGEGLYLDDINPDPRYTGVETSDTGSPDTAFDVVPLPT
                     RPTWFQVRGRDGEGQSSNWSARVLFSPNVAGVAAAAPPRVDRLAGIAPNPANPRADVR
                     FSLSPGAPSTWRLDCFDASGRWMGRIAEGRDDGSGGDRRAAWSARDRTGKDLPSGIYF
                     IRLIHGAARRTAKVAIAR"
BASE COUNT          965 a         2059 c         2053 g         1060 t
ORIGIN      
        1 tgttacttcg acgcttcctt gatcgagagc acctcgatca cttccagcgt cgcctcgtcc
       61 atcatgtagt tgaagttgtc gatcatcgtg acgaacacgt tgtagagcac gatcgagaag
      121 atcgccacga agaggccgcc cgcggtgttg atgagcgcct cagaaatccc gacggcgagc
      181 ttcacggcgt ccaccgagcc cgtgtggccg agggccgcga acgaccggat catcccgatc
      241 accgttccga gaagtccgac catggtggcg atcgaggcga tcgtggcgag cgcgatcagg
      301 ttccgctcga gcagcggcac ctcgagcatg ttcgcctctt ccatcgcctt ctgcacttcc
      361 tggatcttgg cctggcggtc gagcggagca tcgcgcacga tggcgtagcg atcgaggccc
      421 gcgcgcagca cgttcgccag cgagccgcgc tgcttggagc agagctccgc cgcgccgtcg
      481 acgtcgaggg cgtcgatctt ctgacgcacc ttccgtagga acgtgggggc cggctcttta
      541 cccttcgcgc gcatgagcgt gaagaggcgc tccagcgtga aggtgacgga catgatggag
      601 agcatgatca acaaggccac gaggggaccg ccctgcttga tgtactccgg caggaaattg
      661 tagatggcga tcgaaatccc cagcgcgacc accgcgacga tggtgacgaa caaaccctgt
      721 ctcactgcga ttcctcctta gatgccctgg caggcggatc gggtggatcc cgacgacgaa
      781 ggagtatgcc ccaggacccg cgcccggaca acccctgccc ttcgtgtcac ttgaagctct
      841 tgatggcttc ctcttcggtc tcgtaggtct cgaacaccaa ggagagccgg gtcacgacga
      901 aaatgttctg gatcttcttg tccacggcgc acagcttgag ctgcgcgccg cgcttcgcgt
      961 agctcgaatg ccccgcgatc agcacgccca gcgaaacgct gttcatgaac gtggtctgcc
     1021 ccaggttgat caggagcttc ttgttccccg cctctgaaag ctccttgatc ttccccgaca
     1081 actcgtcggt ttcctttccc cccaggagca tccccttggg cgccaggatc gttacgtccc
     1141 ccgacgtacg gacatcgaga ctcattctgc ctccctgtcg gcggcttctg gccgcacggt
     1201 cgctatggtt tcgacccgga tgacgatgga gatgactggg ccgcgcgctt ctgctgcacc
     1261 tgctgctgtg acgcctcgta tttgcggatc aggttcagac cgtcggacgc cggccggttg
     1321 gtcgggtcga ggtcgatcgc cttgttgaac tccaccttgg ccagcgggat gttcttgcac
     1381 ttgaggtagc cctgcgccag ccagatgtgc ccctgcacgt gctcggggtc gaccgcggcc
     1441 gcgcgcgaga gccactggat ggcgttggga cagttgtcca tgaggaggta cgagagcccg
     1501 gcgccgcgga tggcatcgcc gttgacggag tcggccgccg cggcctcctg gaacgactgg
     1561 agcgccgagg gcagcgagtc ggccggcatc tgcatcagga tctggccgat ccagacgtgc
     1621 gcccgcaccg cgttcgcgcg gtcgttcggc cgcagcgcca cggcgcgccg cagatagccg
     1681 accgcttccg gattctcctt cagctgcatc ttggccagtc ccaggttcag gagcgccggc
     1741 acgaattccg gatccaccgc gagcgtcttc tcgaagtacg ggaccgatcc cgcgtagtcc
     1801 tggttcttga aatagaggaa gccgagctgg aagtaggaat cccgcgcgag cgaggtgtcc
     1861 tcctgcgcgg cgctcgtcag catcggcacc gcgcgggtcg tgtcgcccag cgaggcgaag
     1921 atcgtcccga tccggatccg ctcgtcgggc gccagcgtgt cggcgacgtc ggcgtactgg
     1981 gtgcacgcct tcatcgcgtt gtccttcccc tcgtccccct gctcggagta gagcttgcag
     2041 agcgttccca ggatctcctt gtccttcggc cgcttctcga gagccttctc gagatttccg
     2101 atcgccatcg cccgctccgc cggatcgcgg ctgtaggaca gcgcgcgccc gagctccagc
     2161 gagccctgcg cgtcgtcggg catcagctcg acgtacttcg agaatttctc caccgcctcg
     2221 ggatagcgct tcgcgcgata gaacaggcgg cccgattcga ggtacgccgg cgcgtacgtg
     2281 gaatccacgt tgttcgcctc gacgaaggcg cggagccctt cgttgaactc gttcatgccg
     2341 accgaggcgc ggccgtagag gtagtggtat tcggcgttct tcggatccag ctccttggcc
     2401 ttcttgtact ccggaaccgc gaggaagggg atcttcttcc gctcgtagaa tccgcccagc
     2461 tcgagccgga cgcgcaggtt gttcggatcg gtctcccgcg cgcggatgaa gatctcctcc
     2521 gcctccttca tctgcccctt gccctcgagg gcaagggcgg tgccggcgag gaactgcgcg
     2581 cggccggtct tcaagagcgt ccccttgcgg aagactttct ccgcctcgtc gtacttcttg
     2641 tccttcaggt acgcggtccc gagcgagtag agggcggcgg ggtccttggg gtcgaacttg
     2701 accgcggctt cgagcttctc ggtcccctcg gggatcttcc cctgcttgcg gagcaggttc
     2761 ccgagaccgg tcagcgcctc cgtgtatttc ggatccatgc ggatcgcggt ctcgtattcc
     2821 gcgcgcgcgc tgtccgcgtt gttcatccgc tcgagcgccg aggcgatgta gtagtgcggc
     2881 ttggggctgt tgggctccag cctctgggcc cgggagaaga tgtcgagcgc ctgctgggcc
     2941 ttgccgtcct tcagggcggt gacgccctgc ttgatcaggt cggatccgtt gagggcccag
     3001 gccgcggcgg cgaaggcgga gaggccgaga acgaggagca ccaaaccaat acgacgtggg
     3061 acggaattca ctctgcctcc tccgcggtac ggacgatccg aatgggcacg gtagcgggag
     3121 cgtagccgta gcgcgcgacg atcgcctggc cgtcgactcc cgaaatgaac gtgatcagct
     3181 ccgacgcggt ctcccgaacc ttggcgggcg tcgccagggc gatgctccgg agcagcgggt
     3241 aggtgcgttg gagcacggtc tcccgcgtga gcggaatata cggcagcccc ttggcctgtg
     3301 cgatccgcag cacgcgcacg cgatcccccg cggcttcggc gtagggacgc gagacgcacg
     3361 cgatcgcgtt ccggcgcgtg gcgacgacgt ccaccgcctc ctcctccgtg ggcggcgcgt
     3421 agacggtctt ggcgtaggtc ccgccgttca gaagggtctg ctccacgtag gcgtacatgc
     3481 cgagccgcgt gccgctggtc agcgcgatga tcgggcctcc cgccttccat ccgagatcgg
     3541 cccacgacga ggtcgagccc gaatagatcc agccgagctc gctgcgggag agctgttcca
     3601 gcggcgagcc gggattcacg atgacggcga ccgcgtccca ggcgaacggg aaggtctgga
     3661 gtccctcgcg gcgaaccgcg gcctccgcct ccggatcggt cagatcgcgc aacaacaccg
     3721 acatgctcac gtctccgttg accagctgtt ccattccttc ggcagaaccg ttcgtcacga
     3781 cgcggatcga gtccgacgcc ggatagcgct cgcgaaaggc caggatttca gcccgcaaga
     3841 gcggtccgac gtactcggcg ccggcgagaa cgatcggtgt ggaggggggt tcgtcgcgcg
     3901 gcggggatga cctcgggccg catccgctga ccagggcggc gagcacgagg gccaccgcga
     3961 gagcccggac gcggagcgcc cggtcgacac cacgcacgag gtcagagtat ccgtccgggg
     4021 aacccctgtc aacgccgaga cttcctgccg acgcggtccg cgccgtcgcg gggctgtcca
     4081 ctgatccgtg taccccgacg ggaccctgtt gattccgcgc gaaatgagac gggaacgtag
     4141 ggcgcggggt cggcgagtgt caacccaaaa aggcacttgg gaggcgccaa gccacccggg
     4201 agtgctctca gcgggcgatc gccaccttgg cggtgcgccg ggcggcgccg tgaatgaggc
     4261 ggatgaagta gataccgctc gggaggtcct tcccggttcg gtcgcgggcg ctccaggcgg
     4321 cccttcggtc gccccccgag ccgtcgtccc gcccttcggc gatccggccc atccatcgcc
     4381 ccgacgcatc gaagcagtcg agacgccagg tcgacggcgc tcccggcgac aggctgaaac
     4441 ggacgtcggc cctcggattc gcgggattgg gggcgatgcc agcgagccgg tccacccgag
     4501 gcggggccgc cgccgccacg ccggcgacgt tcggcgagaa gaggacccgc gcgctccagt
     4561 tggaggattg cccctccccg tcgcgtcccc ggacctggaa ccaggtgggc cgcgtgggga
     4621 gcggcaccac atcgaacgcc gtgtcggggc tgccggtgtc ggacgtctcg acccccgtgt
     4681 agcgcgggtc ggggttgatg tcgtccaggt agaggccctc gccgtgattc gcggcgtcgg
     4741 tcacgcagcg aaaccgcacg agcgcctgct tccccacgta cgggagcagg ctgaaggcgg
     4801 tgcgcacgaa cacgccgccg ctcgaaccgg tcacgccgaa tccctcgttc tggcccgagg
     4861 gattcaagtt cgtggtgtgc gtgccggcga gcgagtgcca cgttccgcct ccgtccacgg
     4921 aggcctgcgc gtaccaatag tcgaaccccg actcgagatc ccaccaggcc caggccacca
     4981 ggctgtcccc catgcccacg tcgaacgacg cacgggagct gagaatgctc tcccggttgt
     5041 ccgaagaccc cgacccgtag ctgtggctac cgtccgcctt gcgcgcggtg gaccaggcga
     5101 agcggagggt gtcccagtcg gccgtccccc cttcggcgtc gtcgaggccg cggagcacct
     5161 gcgcgatccg ccggacgtcg tgccgcgccg ccggattcgc cgggtcggga acggggtagc
     5221 tccagcgcat ctgggccgtt ccgcccgggc cgaccaccac ggccggagac tcgggccggg
     5281 cgggaccgag gacgcgcttc ggttcgtcgg cgtagcggag gaccgtcagc acgccgcccc
     5341 agttggcctc gcacgtgggc ccgatcaaag cgtcgggcgg gtcgaagccg ccctcttcct
     5401 cggtgttcag ctcgatggtg tatccgtaga cgaccggctt gtcggacgcg tccccgtaca
     5461 gccagtcgtc gatgccgccg ttcgtgaggt agatcgcgtt cgacttcgga ttccccacgc
     5521 ggtagccgtt gtaggatccg atcgaatcgc ccagggccga gaagacggcg ttgtcgggca
     5581 cgtcgagccg gtcgtagccc cacgggtaga gcaccaggtc gccgtaggag tggaacgaga
     5641 gcgacaccgt gaaacgccgg gaaccgatga aatcccggag cacctgggtc tcgggctccg
     5701 agaaggcggc ggtgccgcga taggtttcgg cggtggtcgt ggggctggag ccgacgtcgt
     5761 cgtaggccca ctcgaaccca tagttccggt tcagatccac accgaacgaa ccgtcggcgt
     5821 tgggccgccg gttcttccgc caccagccgg tggactggcc gcccgagtgc tgctccacgt
     5881 acacgtagcc gtcgggattg gccaccggca ggatccagat gcgccgcgtg tcgacgacct
     5941 gggtgaggaa cgggtcggtg ccgtacccgt cgagcagccg ccgcatcgcg aacagcggca
     6001 cctcgacgct catcagctcg cgggcgtgca ggcagccgtc cacgagcacc tcgggctctc
     6061 cctcgtcgat gccagggttg tcggagatct cgatcgcgac gagcggcctg ccctcgaggc
     6121 tcaggcccag cgtgtcg
//
LOCUS       DASSLZ010000233         8611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1084898, whole genome shotgun sequence.
ACCESSION   DASSLZ010000233 DASSLZ010000000
VERSION     DASSLZ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084898"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VFM00_08765"
     CDS             <1..1023
                     /locus_tag="VFM00_08765"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7498379.1"
                     /translation="ERRVLSRDVRWLKGTTGADDADLLRAFVALYYAGSESVPSTLIA
                     EAADGDRELLESYLARRADAPVRVRPPRGTGEQALARMARRNAAFLLAKEKGEATKGL
                     EGASGEEALDLQRALGLPAMPRRIRCFDVSNLFGTHVVASMVTFLDGAPYKSEYRRYR
                     IRAVAGQDDFASMAEVVSRHAQRVAIQDLSAADLIVIDGGVGQLESARRAASGTPLET
                     VPIVGLAKRLEEIVVPGRRDPLRLSRRAPGLKLLMRVRDEAHRFAVTYHRTLRGRAAS
                     ESALDQVKGLGPKRRAMLLARFGSVTRLRNEPLETIASVPGIGKRMAERILETVGAGS
                     EGGSGS"
     gene            1020..1652
                     /locus_tag="VFM00_08770"
     CDS             1020..1652
                     /locus_tag="VFM00_08770"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7498380.1"
                     /translation="MKAKKRAAKSERRGQLQLEPLAVASVRTPAGTITVVCSPSGLRE
                     VHLGAAETRRREARARGIRFVRRPGWTAGAKAAVERFVRGGPIPAGLPLDLAQGTAFQ
                     RRVWDATRAVPWGEVVSYAEIARRAGAPRAVRAVGNALGDNPVPIVVPCHRVVHSGGS
                     IGGFTSGLDWKRYLLALEGGQLEMELRKPRRGVLGLLRAARRAAGRAGGR"
     gene            1649..2593
                     /gene="xerD"
                     /locus_tag="VFM00_08775"
     CDS             1649..2593
                     /gene="xerD"
                     /locus_tag="VFM00_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET7498381.1"
                     /translation="MTPAPKKNPGGAEPEAKRAKSVLGPLLERFRDHLAEERRLSVNT
                     VRAYGRDLAQYDRYLAGQGRRDASAVSSADIERYVAAGGWSASTVARKIAALRSFHEF
                     LRRRGLAGDNPALPVRPPRRSRPLPDVLAVEQVEALLKAPRGESPQAVRDRALLETAY
                     ATGLRASELVKLALEEVDLEERLVRCVGKRQRERIVPFGSQARAALVRYLDGARPLLT
                     RHRAERSVFVTRLGRPFTRMGYWKLLRGYARAAGIDRPVSPHTLRHSCATHLLEGGCD
                     LRVVQEFLGHRSIETTQIYTHLDRNYLREVHAKFHPRA"
     gene            2634..3407
                     /locus_tag="VFM00_08780"
     CDS             2634..3407
                     /locus_tag="VFM00_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498382.1"
                     /translation="MRDRVVGALALGPLVLIAVAWAPSAHASDLNAEASARYAAAARE
                     GGTLLLRDGEALFVSASSEAGRAARRQPQPVPEKPARILERRQRPNTISVGIQGQYGG
                     IRGNSRLADGFDNGPGYAFRFRYMVSPSSAVGFSFEHQRFGSINPPINVQGDFADSHV
                     VATTVAIEGVFYVHRERMAMPYFVGGFGYASPDIIFSEDQSSRVNEGAFAVLGAGFER
                     FIRPRFSADVSIRGYAMVSNSQFTSMGQLSLGIHLYPGD"
     gene            3413..4801
                     /locus_tag="VFM00_08785"
     CDS             3413..4801
                     /locus_tag="VFM00_08785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7498383.1"
                     /translation="MRVTFWGAARTVTGSKHLLAGRQGTVLLDCGLFQGRRAESTARN
                     QSLPFQPGSVDALVLSHAHIDHSGAIPLLVKSGFEGPIHATAATADLCRAMLLDAAHI
                     QVKDADFMNRRRGGRHGRERVIPLYDTTDVERALEQFRPHAYGETFEVRPDLSVAFHE
                     AGHILGSAHVEARWKENGRSGSLVYTGDLGRLHRPIIRDPAPLPHADHLITEGTYGGK
                     THPDEPSLLAALERVVREAVDRGGRIVIPAFAVGRTQEVVHALNQLEAEKRIPTLPVY
                     LDSPLANEVQAIVARHTEVFDEETCAALARGEDPLGMRRVIAVREPAESMKLNDKPGT
                     FVTIAASGMCEAGRILHHLHHTVEDPRHTILIVGYQAEGTLGKRLVDRLPEVRIFGDP
                     HTVRARVEVLNGFSAHADHPALVAQAEHCRPSAGCAIVHADLERAEALRAALPSGWNS
                     RIPLEGETWDLG"
     gene            4798..6792
                     /locus_tag="VFM00_08790"
     CDS             4798..6792
                     /locus_tag="VFM00_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HET7498384.1"
                     /translation="MSRDGGHPRSPGFRVGDRVVHRAFGEGLIVEVRERDGFEVLEIA
                     FANGIRRLTSLYPLEPARGAGRATGPPSVASAPAAASVRLGLDLPTRGQFRIAPGAED
                     LVQRLLTGPYDAAREHELRLRVERYSLHRGFDRLLCLDRLHDVQHYGHQIQACATALR
                     RMRGRALLADEVGLGKTIEAGLVLKEYLLRGLVQRALVLVPASLVEQWDQELRRKFDL
                     EFAILKHGTPWWEEPLLLASMDTAKLLRHREKVAAASFDLVIVDEAHRLKNQKTLAWK
                     FLDRLAPRYLLLLTATPVQNDLHELYNLVQLLRPGLLGTYRTFGREFMRRGDRRMPQN
                     TQRLANLLSETMIRTTRASTSIRFPKREVTNVLFDQSPAERALYENVTRLVRRLVQSQ
                     RENRRGAIKLTLLTLQKEIGSSTFAAAPTLAKLLARETPGQARNELEALLHGARAIET
                     NAKFEGIQKLVTSSRDKFVVFTQFRATLEFLARSLRAMGVKTAVFHGALSLREKEAAI
                     AQFKDRARVLVSTEAGGEGRNLQFCHLLVNYDLPWNPMRIEQRIGRLHRLGQTHDVHV
                     FNFTARNTVENYVLEIVHEKINLFRAVIGDLDMVLGPYAEAGSFEDAVFTIWANAKGD
                     RDLEREFGKLGAALLVGRKRYEAVEDLDRKLLDGEDNGGT"
     gene            6779..7585
                     /locus_tag="VFM00_08795"
     CDS             6779..7585
                     /locus_tag="VFM00_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498385.1"
                     /translation="MAGRKRSGEGPRAELPEILAAILETRGAEVARRGGGVWEAALPE
                     PLAKELGTDRVRLVASPAGRAARGAEADAAMTERILLIGRSMGTVTRLVARAPLPKGI
                     EAPPQWVRLHWRIRYGSDDLPEELLAQTLPIAATSGVRAPRDGVLRAPTEKEADLLDT
                     PDPERLTRAWHRALRQLELRIRTRLRPHEDRVRRELHREMRTLSVHFRSLIAEERAGR
                     RRRPEDGEAGRMLQLKEDWERKLAAAVRQRAFDTEARLVAAALITLLPPV"
     gene            7654..8358
                     /locus_tag="VFM00_08800"
     CDS             7654..8358
                     /locus_tag="VFM00_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7498386.1"
                     /translation="MSTHLVKHQKLTKRQMKEDPLVTAAFRATEVWERFGRTILIAAG
                     GILVLALLVFFVLRTRAQAEQRAMGDLFRAQVALTQGDAQSAAPMLKELVDNSPGTRA
                     ARDAMLLLGDAMMTQRNPKEAATWYQKFVDKAGGDKALERAGYLGLGAALEDGGQFVP
                     AADAYAKSAERAVSDNDRGRAMLSEARSLLRAGQTRKAMEIYQKTTTLPGAEVPIKDA
                     ANTHLGELSVTAPAAP"
     gene            8369..>8611
                     /locus_tag="VFM00_08805"
     CDS             8369..>8611
                     /locus_tag="VFM00_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET7498387.1"
                     /translation="MPARPILLALVWHMHQPSYRDPVSGRSLLPWTRLHATKDYRDMV
                     DLLRAYPRVHCTFNLTPVLLDQLERIAAGDEDEALRL"
BASE COUNT         1240 a         3046 c         3144 g         1181 t
ORIGIN      
        1 gagcgccggg tgctctcgcg ggacgtgcgc tggctcaagg gcaccacggg cgccgacgac
       61 gccgacctcc tccgcgcctt cgtcgcgctc tactacgcgg gctcggagag cgttccctcg
      121 acgctcatcg ccgaggccgc ggacggcgat cgcgagctct tggaatcgta tctcgcgcgg
      181 cgcgccgacg cgccggtgcg ggtccggccg ccgcgcggca cgggagaaca ggcgctcgcg
      241 cgcatggcgc ggcggaacgc ggcgttcctg ctcgccaagg agaagggcga ggcgacgaag
      301 ggcctcgagg gcgcgtccgg cgaggaagcg ctggatctgc agcgcgcgct gggcctgccg
      361 gcgatgccgc ggcgcatccg ctgcttcgac gtctcgaacc tcttcggaac gcacgtcgtc
      421 gcttcgatgg tgacgtttct cgacggggcg ccgtacaagt cggaatatcg ccggtatcgc
      481 atccgggccg tcgccggcca ggacgacttc gcctccatgg ccgaggtggt gtcgcgccac
      541 gcgcagcggg tcgcgatcca ggatctctcg gcggccgatc tgatcgtgat cgacggcggc
      601 gtcgggcagc tggaatcggc gcgccgcgcc gcgagcggga cgccgctcga gacggtcccg
      661 atcgtggggc tggcgaagcg gctggaggag atcgtggtcc cgggccggcg tgatccgctc
      721 cgtctgtcgc gccgcgcgcc cgggttgaag ctgctgatgc gggtgcgcga cgaggcgcac
      781 cggttcgcgg tgacctatca ccgcacgctg cgcgggcgtg ccgcgagcga gtcggccctg
      841 gatcaggtga aggggctcgg gcccaagcgc cgcgcgatgc tgctcgcgcg cttcggctcg
      901 gtgacgcggc tccgcaacga gccgctcgag acgatcgcct ccgtgcccgg aatcggaaaa
      961 cggatggcgg aacggattct cgagacggtc ggggcgggga gcgaaggggg gagtgggtca
     1021 tgaaggcgaa gaagcgcgcg gcgaagtcgg aacggcgcgg gcagctccag ctggagccgc
     1081 tcgcggtcgc atcggtccgg acgcccgcgg gtacgatcac cgtcgtctgc agcccatcgg
     1141 ggctgcgcga ggttcacctg ggcgccgccg agacgcggcg gcgggaagcg cgcgcgcggg
     1201 ggatccgctt cgtgcggcgg ccgggctgga ccgccggcgc caaggccgcg gtggagcgct
     1261 tcgtgcgcgg cggtccgatc cccgccggcc tgccgctcga cctggcccag ggcacggcgt
     1321 tccagcgccg cgtgtgggac gcgacccgcg ccgtgccgtg gggcgaggtc gtctcgtacg
     1381 cggagatcgc ccggcgcgcc ggagcgccgc gcgcggtgcg cgcggtgggg aacgccctcg
     1441 gcgacaaccc ggtgccgatc gtcgtgccgt gccaccgcgt ggtgcactcc ggcggctcga
     1501 tcggcgggtt cacgtcgggc ctcgactgga agcgctacct gctcgccctg gaggggggac
     1561 agctggagat ggagctgcgg aagccgcgcc ggggcgtgct ggggctgttg cgcgccgcgc
     1621 ggcgcgcggc cggccgggcg ggaggtcgtt gacccccgcg ccgaagaaga accccggcgg
     1681 cgcggagccg gaggccaagc gtgcgaagag cgtgctgggg ccgctcctcg agcgtttccg
     1741 cgaccacctg gccgaggagc gaaggctcag cgtgaacacc gtgcgcgcct acggccgcga
     1801 tctggcccag tatgaccgct acctcgcggg ccagggacgg cgggatgcgt cggccgtctc
     1861 ctcggccgac atcgagcgct acgtcgcggc gggcggctgg tccgcctcca ccgtggcgcg
     1921 gaagatcgcg gcgctccgat ccttccacga gttcttgcgc cggcggggtc ttgcgggaga
     1981 caatcccgcg cttccggtcc gcccgccgag acggtcgcgt cctttgcccg acgtgctcgc
     2041 ggtggagcag gtggaggcgc tcctgaaggc gccgcgcggc gagtctccgc aggcggtgcg
     2101 cgaccgggcg ctcctcgaga ccgcgtacgc gaccggcctc cgcgcgagcg agctggtgaa
     2161 gctcgcgctg gaagaggtcg acctggagga gaggctggtt cgctgcgtgg ggaagcggca
     2221 gcgggagcgc atcgtcccct tcggtagcca ggcgcgcgcg gcgctcgtcc gctacctgga
     2281 cggggcgcgg ccgctcctga cgcggcaccg cgcggagcgc tccgtcttcg tgacgcgcct
     2341 gggacggccg ttcacacgca tgggctactg gaagctcctt cgcggctacg cgcgcgccgc
     2401 ggggatcgac cggccggttt cgccgcacac gctccggcat tcgtgcgcca cgcacctcct
     2461 cgagggggga tgcgacctgc gcgtcgtgca ggagttcctg gggcaccggt cgatcgagac
     2521 gacgcagatc tacacccatc tggaccggaa ctacctgcgc gaggtccacg cgaagtttca
     2581 cccgagagcc tgaacgcacg ccggacgaac gccggagccg gggagggatg gagatgagag
     2641 atcgagtcgt cggggcgctg gcgctgggcc cgctggtcct gatcgcggtc gcatgggcgc
     2701 cgtccgcgca cgcatcggac ctgaacgccg aggcatcggc gcggtacgcg gcggccgcgc
     2761 gcgaaggggg cacgctgctc cttcgcgacg gggaggcgct gttcgtctcc gcgtcgtccg
     2821 aggcggggcg cgccgcgcgg cggcagcctc agccggttcc cgagaagccg gcgcgcattc
     2881 tcgagcgccg gcagcgcccg aacacgatca gcgtcggcat ccaggggcag tacggcggca
     2941 tccgggggaa ctcccgcctc gccgacggat tcgacaacgg tcccggctac gcgttccgct
     3001 tccgttacat ggtctcgccc agctcggcgg tcggattctc gttcgagcac cagcgcttcg
     3061 ggagcatcaa ccctccgatc aacgtccagg gggacttcgc cgacagccac gtcgtggcga
     3121 cgaccgtcgc gatcgagggc gtcttctacg tccaccgcga acgcatggcc atgccctatt
     3181 tcgtcggcgg attcggctac gcctcgcccg acatcatctt ctcggaagac cagtccagcc
     3241 gcgtcaacga aggggcgttc gccgtcctgg gagcgggatt cgagcgcttc atacgccccc
     3301 gcttctcggc cgacgtctcg atccgcgggt acgcgatggt ctccaactcc cagttcacgt
     3361 cgatgggcca attgagtctc gggatccatc tctaccccgg agactagccc gcgtgcgcgt
     3421 caccttctgg ggcgcggcac gcaccgtgac cggatcgaag cacctcctgg cgggacgcca
     3481 gggaacggtg ctgctggact gcggcctctt ccaggggcgc cgcgcggagt cgacggcgcg
     3541 gaatcagtcg ctcccgttcc agccgggctc ggtggacgcg ctcgtcctct cgcacgcgca
     3601 catcgaccac tcgggcgcga tcccgctgct cgtgaagagc ggtttcgaag ggccgatcca
     3661 cgccaccgcc gcgacggcgg acctctgccg cgcgatgctc ctcgacgcgg cgcacatcca
     3721 ggtgaaggac gccgacttca tgaaccgccg ccgcggaggc cgccacggac gcgagcgggt
     3781 gatcccgctg tacgacacga ccgacgtcga gcgcgcgctg gagcagttcc gcccgcacgc
     3841 ctacggggaa acgttcgagg tccggcccga cctgtcggtc gcgttccacg aggcggggca
     3901 catcctgggc tcggcgcacg tggaggcgcg ctggaaggag aacgggcgct ccggatcgct
     3961 ggtctacacg ggagacctgg ggcggctgca ccggcccatc atccgcgacc cggccccgct
     4021 cccgcacgcc gaccacctga tcaccgaggg gacctacggc gggaagaccc accccgacga
     4081 gccctcgctc ctggccgcgc tcgagcgtgt ggtgcgcgag gccgtggatc gaggcggccg
     4141 gatcgtgatc cccgccttcg ccgtcggacg gacccaggaa gtggtgcacg cgctgaacca
     4201 gctcgaggcc gagaagcgga tccccacgct ccccgtctac ctggacagcc ccctcgccaa
     4261 cgaggtgcag gcgatcgtgg cgcggcacac ggaggtcttc gacgaggaga cctgcgcggc
     4321 gctcgcgcgc ggcgaggatc cgctcggcat gcggcgcgtg atcgccgtgc gggagccggc
     4381 ggagtcgatg aagctgaacg acaagcccgg tacgttcgtg acgatcgcgg cgtcggggat
     4441 gtgtgaagcg gggcggatcc tccatcatct ccaccacacg gtggaggatc cgcgccacac
     4501 catcctgatc gtcggctacc aggccgaggg gacgctgggg aagcgcctgg tggaccggct
     4561 gcccgaagtc cggatcttcg gggacccgca caccgtgcgc gcgcgggtag aggtgctgaa
     4621 cggcttcagc gcccacgccg accatccggc cctggtggcg caggccgagc attgccgccc
     4681 ctcggccggg tgcgccatcg tgcacgccga cctcgagcgg gccgaggcgc ttcgcgccgc
     4741 gctcccctcg gggtggaaca gccgcatccc gctcgagggg gaaacgtggg acctcggatg
     4801 agccgcgacg gcggacaccc gcgctcgccc ggattccggg tgggggaccg cgtcgtgcat
     4861 cgcgcgttcg gcgaagggct gatcgtcgag gtgcgggagc gggacggctt cgaggtgctg
     4921 gagatcgcgt tcgcgaacgg catccgccgc ctgacgtcgc tctatccgct ggagcccgcc
     4981 cgcggcgcgg ggcgcgcgac cggcccgccc tccgtcgcgt cggctccggc cgccgcctcg
     5041 gtgcgcctcg gtctcgacct gccgaccagg ggacagttcc ggatcgcgcc gggcgccgag
     5101 gacctggtcc agcgcctcct caccggcccc tacgacgcgg cgcgcgaaca cgagctgcgc
     5161 ctgcgcgtcg agcgctactc gctccaccgc ggcttcgacc gcctgctctg cctggaccgc
     5221 ctgcacgacg tccagcacta cggccaccag atccaggcgt gcgccacggc gcttcgccgg
     5281 atgcgggggc gcgcgctcct cgccgacgaa gtggggctgg ggaagacgat cgaggcgggg
     5341 ctcgtgctca aggagtacct gctgcgcggc ctcgtccagc gcgcgctggt cctggtgccg
     5401 gcgtcgctcg tcgagcagtg ggaccaggag ctgcggcgga agttcgacct cgagttcgcc
     5461 atcctgaagc acggcacccc ctggtgggag gagccgctcc tgctcgcctc gatggacacg
     5521 gcgaagctcc tgcgccaccg ggagaaggtg gccgcggcct cgttcgacct cgtgatcgtg
     5581 gacgaggcgc accggctgaa gaaccagaag acgcttgcct ggaagttcct cgaccggctg
     5641 gcgccgcgct atctcctgct cctcaccgcc acaccggttc agaacgacct gcacgagctg
     5701 tacaacctgg tccagctgct ccgccccggg ctcctcggta cctaccggac gttcggccgg
     5761 gagttcatgc ggcgcggcga ccggcgcatg ccgcagaaca cccagcggct cgcgaacctc
     5821 ctctccgaga cgatgatccg cacgacgcgc gcgagcacct cgatccgctt cccgaagcgc
     5881 gaggtcacga acgtcctgtt cgaccagagc cccgccgagc gggcgctgta cgagaacgtg
     5941 acgcgcctcg tgcgccgcct ggtgcagtcg cagcgggaga accgtcgtgg ggcgatcaag
     6001 ctcacgctcc tcacgctcca gaaggagatc ggctcgagca cgttcgccgc ggcgcccacc
     6061 ctggccaagc tgctcgcgcg cgagacgccc gggcaggcgc gcaacgagct ggaggcgctg
     6121 ctccacggcg cgcgcgccat cgagaccaac gcgaagttcg aggggatcca gaagctcgtg
     6181 acctcctcgc gcgacaagtt cgtcgtcttc acccagttcc gcgccacgct cgagttcctg
     6241 gcgcggtcgc tgcgcgcgat gggggtgaag acggcggtct tccacggggc gctctccctg
     6301 cgcgagaagg aggccgccat cgcgcaattc aaggatcgcg cccgcgtgct cgtgagcacg
     6361 gaggcgggcg gcgaagggcg gaacctccag ttctgccacc tgctcgtgaa ctacgacctc
     6421 ccctggaacc cgatgcggat cgaacagcga atcgggcgtc tccaccggct cgggcagacg
     6481 cacgacgtgc acgtcttcaa cttcacggcg aggaacaccg tcgagaacta cgtgctcgag
     6541 atcgtgcacg agaagatcaa cctcttccgg gccgtgatcg gggacctgga catggtgctc
     6601 ggcccctatg ccgaggccgg gtcgttcgag gacgccgtct tcacgatctg ggcgaacgcg
     6661 aaaggggacc gggacctgga gcgcgagttc ggcaagctgg gcgcggcgct cctggtggga
     6721 cgcaagcgct acgaggcggt cgaggacctc gaccggaagc tcctcgacgg agaggacaat
     6781 ggcgggacgt aagcgatccg gcgagggccc gcgcgccgag ctgcccgaga tcctggcggc
     6841 catcctcgag acccggggcg ccgaggtggc gcggcgcggg ggaggcgtgt gggaagcggc
     6901 acttcccgag ccgctcgcca aggagctcgg caccgaccgg gtgcggctgg tggcctcgcc
     6961 cgccgggcgc gccgcccgcg gcgccgaggc ggacgccgcg atgaccgagc ggatcctcct
     7021 gatcggacgc tcgatgggca cggtgacgcg gctggtcgcg cgcgcccccc ttcccaaggg
     7081 aatcgaggcg cctccgcaat gggtgcggct ccactggcgg atccggtatg gcagcgacga
     7141 cctccccgag gagctgctcg cgcagaccct gccgatcgcg gccacgagcg gggtccgcgc
     7201 gccgcgtgac ggggtgctgc gcgccccgac cgagaaggag gctgaccttc tcgacacgcc
     7261 cgatcccgag cggctcacgc gcgcctggca tcgcgcgctc cggcagctcg agcttcggat
     7321 ccggacgagg ctccgcccgc acgaggaccg cgtgcggcgc gagctgcatc gcgagatgcg
     7381 cacgctgagc gtgcacttcc ggtcgctgat cgccgaggag cgcgcggggc ggcgccggcg
     7441 ccccgaggac ggggaggcgg ggcggatgct gcagctgaag gaggactggg agcggaagct
     7501 cgccgcggcc gtgcggcagc gcgccttcga caccgaggcg cggctcgtgg ccgccgcgct
     7561 gatcaccctg ctcccgcccg tttgacactc cccggtcgcc ggggtagact cgacgactcg
     7621 ttcttcgacg ctcatccctt ggagtctcgc gccatgtcga cccatctcgt gaagcaccag
     7681 aagctcacca agcggcagat gaaggaggat ccgctcgtca ccgcggcgtt ccgggccacc
     7741 gaggtctggg agcgcttcgg acggaccatc ctgatcgccg ccggcgggat tcttgttctc
     7801 gccctgctcg tcttcttcgt cctccgcacc cgggcgcagg ccgagcagcg cgcgatgggc
     7861 gacctcttcc gcgcgcaggt ggcgctgacg cagggagacg cgcagtcggc ggcgccgatg
     7921 ctgaaggagc tggtcgacaa ctctccgggg acccgggcgg ctcgtgacgc gatgctcctc
     7981 ctcggcgacg cgatgatgac gcagcgcaat ccgaaggaag cggccacctg gtaccagaag
     8041 ttcgtggaca aggcgggcgg cgacaaggcg ctcgagcgcg ccggctacct cggactgggc
     8101 gcggcgctcg aggacggggg acagttcgtg cccgcggccg acgcctacgc caagtcggcg
     8161 gagcgcgccg tcagcgacaa cgatcgcggg cgcgccatgc tgtccgaagc gcggagcctc
     8221 ctgcgggccg gccagacccg gaaggccatg gagatctacc agaagaccac gaccctcccg
     8281 ggcgcggagg tgccgatcaa ggacgcggcc aacacgcact tgggagagct ctcggtcacc
     8341 gcacccgcgg cgccctgaac cggcgccgat gccggcgcgt cccatcctgc tggcgctcgt
     8401 ctggcacatg caccagccga gctaccgcga ccccgtgtcc gggcgttcct tgcttccctg
     8461 gacgcggctc cacgcgacga aggactaccg cgacatggtg gaccttctgc gcgcctaccc
     8521 ccgggttcac tgcacgttca acctgacccc cgtcctgctc gatcagctcg agcggatcgc
     8581 ggcgggggac gaagacgagg cgctgcgcct c
//
LOCUS       DASSLZ010000234         5291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1343605, whole genome shotgun sequence.
ACCESSION   DASSLZ010000234 DASSLZ010000000
VERSION     DASSLZ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343605"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1948)
                     /locus_tag="VFM00_08810"
     CDS             complement(<1..1948)
                     /locus_tag="VFM00_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET7498388.1"
                     /translation="MSRRAMLRRVVGGTLAWATAAAALAVAAALFFGAHAGRAVARQG
                     EHRLLDLAAALAGSIAPEEADIAVQSAPEARFAREIRSQLVALRRNDASIRRILLLEA
                     GSKGDPRVLLEVDGGRAIEPAGAGAGAAPEPRPDPRYHAPAGISLEEADRKPVVSPAA
                     GAAVIAAFAPVKDERGRTVAVAGIEMDGGAIAAAAKARRAQTAAAAGVLVLGIASFAA
                     WRARRGLAALDRLRGLRAQVAIYRVGDALARAESDEELVRLALDAIASGSGIDRWTMY
                     LRDAPGARFRRFAERGAEAGGKAPAEPRPAGPGVVSIPLVDGAETVGVLQCLLPGGRE
                     PESDELALFRWMATQVLVGLKRIRMERRDQMLALFTMGTGEILLGLDLGGLVTYANAA
                     AERALGAAPGGLTGRPLESMAMLGAGEEPFGLVGALDGEREFSGEVCFVRADGSRFPA
                     EVRLSPAVDRQGKLSALVLLGHDVTERREREADLENRTRELALLNEQLQRAVAELEEA
                     RQAQSEFVANTSHELRTPLNAVIGFASLIEQGNHESEAEARDFAQRIRTSAEHLLGLL
                     NDILDLAKVEAGRFQLSMTDGDVRGPVREALDAVASLAASRGLAIAAELPEGPLPARL
                     DPVRARQVLLNLLGNALKYTDKGGV"
     gene            2143..3300
                     /locus_tag="VFM00_08815"
     CDS             2143..3300
                     /locus_tag="VFM00_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498389.1"
                     /translation="MRSIPVAALMLALAATLLGGCSERASRITGNERLLRGDQGLGTT
                     IQSDTLVDRDTYVTPATTAVRGPALLVGILGTYESRALFRPSTWTLPDTLVTTVIDTI
                     RFRVDFDPRVDRYLTGNRVLALNRAAAWDTTNVGWPGPALGPLLGTSPDAQAPFTIEL
                     GASAISQVRAWAADPTFPGFVLSIDSGTGVRGFLAGTGRIEIVYHTTLAPTTHVTKVT
                     RLPTDLSIQTPAAPASGSESTLLLGGLFQSEVILRAPVAPPPAGFSINAARFVAHLLG
                     PVSPDTVEIKAYRIRNAWTEGVSVDSTLGLDAKPLETLTGVRVVAGDSLTIPIPISLA
                     REWSADSTSNKGILIRVTNSFYASEIEIGSRESATPPVLRVNTTSPPPGRF"
     gene            3302..4540
                     /locus_tag="VFM00_08820"
     CDS             3302..4540
                     /locus_tag="VFM00_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498390.1"
                     /translation="MSPLRKSWMARLRPAAPFLAAALIAIPASVRASSAYSAGGLGEP
                     SLEEPARLRALGGAGAAEHGPRAFSLVNPASVAEVDHLILEGTVMPSMRRVSALSFPG
                     ETARETVVPSLRALVRVPGRFTLGGAYLVGTNGRFGIDRPESSGAPSTLRIDGAGGID
                     LIRITLARRLSPSVNLGLDYEVIVGSFREDWARDFVDPALITTRDTLETTWERQGRWR
                     FGAQVHRGTWSLGGVYELARRLPLATTQRTAGTFVRQEHRNLTIPSGFAVGGMAPITD
                     RLRAVGQYRRADWSRESLLSDRVDFRAEQRWSFGLELLPAADATRFLTRLPLRVGASV
                     LTWPDLLVRADATDISGGTAGVKEVAFSLGTGLRSQDRGGAVDLSLEAGSRGDKDALG
                     VSEKFLRLAISLQVSDDTWK"
     gene            complement(4511..>5291)
                     /locus_tag="VFM00_08825"
     CDS             complement(4511..>5291)
                     /locus_tag="VFM00_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498391.1"
                     /translation="ADRWSNPWGDERWGAHAATAAAVRGGLRAASRMAALFGETARAR
                     AWLAASDMLGRAVSRALYDPERGRFLRSVVMEEGERRLDATLDATLLWLGLFDDLEPE
                     DPRVRATCAAVRGALWVRSGVAGLARHEADAAGPGPDRDRAAGRPSVAPTLWLAQHMI
                     RVARKSQDLEEARILLLWAAARAEGTGLLPERLDPASGGADGICPSLDATAAFVTTVL
                     DYGERARLLARCDRCGEPAPARRARRVADRVASLPGVVADL"
BASE COUNT          701 a         1997 c         1879 g          714 t
ORIGIN      
        1 gcacccctcc cttatcggtg tacttgaggg cgttcccaag caggttcagc aggacctgcc
       61 gggcccgaac cgggtccagc cgggcgggga gcgggccctc cggaagctcg gcggcgatcg
      121 cgagcccccg gctcgccgcg agcgaggcga cggcgtcgag cgcttcccgc accgggccgc
      181 ggacgtcccc gtcggtcatg gagagctgga agcgccccgc ctcgaccttc gccaggtcca
      241 ggatgtcgtt caggagcccg aggaggtgct cggcgctcgt gcggatccgc tgcgcgaagt
      301 cgcgggcctc agcctcggac tcgtggttgc cctgctcgat caggctcgcg aagccgatga
      361 cggcgttgag cggcgtgcga agctcgtgcg aggtgttcgc gacgaactcg ctctgggcct
      421 gacgcgcctc ctccagctcg gcgaccgcgc gttggagctg ctcgttcagg agcgcgagct
      481 cgcgcgtgcg gttttcgaga tcggcctcgc gctcgcggcg ctcggtcacg tcgtggccca
      541 ggagcacgag ggcgctcagc ttgccctgcc ggtccaccgc tggggagagg cgcacctcgg
      601 cggggaagcg ggagccgtcg gcgcgcacga agcagacctc gcccgagaat tcgcgctccc
      661 cgtccagcgc gccgaccagc ccgaagggct cctcgccggc cccgagcatc gccatcgatt
      721 cgagcggccg tccggtgagg ccgccgggag cggcgccgag cgcgcgctcg gcggccgcgt
      781 tcgcgtaggt caccaagccg ccgaggtcca gtccgagcag gatctcgccc gtgcccatcg
      841 tgaacagcgc gagcatctga tcgcgccgct ccatccggat ccgcttgagt ccgaccagga
      901 cctgcgtggc catccagcgg aagagcgcga gctcgtccga ttccggctcg cgtccgccgg
      961 gaagcaggca ctggagcacg ccgaccgtct ccgcgccgtc cacgagcggg atcgacacga
     1021 cgcccggccc cgcgggccgg ggctcggcgg gagccttacc gccggcctcg gccccgcgct
     1081 cggcgaatcg gcggaagcgc gccccgggag cgtcgcgcag gtacatggtc cagcggtcga
     1141 tcccgctccc gctcgcgatg gcgtccaggg ccagccgcac cagttcctcg tccgactccg
     1201 cgcgggcgag cgcgtcgccg acgcggtaga tcgcgacctg ggcgcgcagg ccgcgcaggc
     1261 ggtcgagcgc ggccagcccg cgccgcgcgc gccaggcggc gaaggaggca atgcccagca
     1321 ccagcacacc ggccgcggcc gccgtctgag cgcggcgggc cttagcggcc gcggcgatcg
     1381 cgccgccgtc catctcgatc ccggcgaccg cgaccgtgcg gccccgctcg tccttaacgg
     1441 gcgcgaacgc cgcgatcacc gcggcgccgg ccgcggggga gaccaccggc ttccggtccg
     1501 cctcctccag cgatatgccg gcgggggcgt ggtagcgcgg atcagggcgc ggctcgggcg
     1561 ccgcgccggc gccggcgccg gccggttcga tcgcgcggcc gccgtccacc tcgagcagga
     1621 ccctcggatc ccccttcgac cccgcctcca ggaggaggat ccggcgtatc gaggcgtcgt
     1681 ttcgacgcag cgcgacgagc tgcgaccgga tctcgcgcgc gaagcgggcc tcgggggcgc
     1741 tttgaacggc gatgtccgct tcctcgggag cgatggagcc ggcgagcgcc gccgcgaggt
     1801 cgagcaggcg atgctccccc tggcgcgcga cggcgcgccc ggcgtgagct ccgaaaaaga
     1861 gcgccgccgc cacggccagc gccgccgcgg cggtcgccca ggccagcgtt ccgcccacga
     1921 cccggcgcaa catcgcgcgg cggctcatgc cgcccgccga ttcgacggac cttggagcgg
     1981 gcaaccgtgg ttccaccatg cgattcactc tcccggggca agacggaaag gggctcttgg
     2041 cctatcggca tcaagcgtcg gaaactggag cgggaagggg aggcgaggat tccgcgcggt
     2101 cgttctagcg ttcgaagcgc caacgggata ctatctgccc ccgtgcgttc gatccccgtc
     2161 gccgccttga tgctcgcgct cgccgccacc ctcttggggg ggtgctcgga gcgcgcgtcg
     2221 cgcatcaccg gcaacgagcg gctcctccgc ggcgaccagg ggctggggac cacgatccag
     2281 tcggatacgc tcgtggaccg cgacacgtac gtgacgccgg ccacgaccgc cgtgcggggc
     2341 ccggccctcc tcgtgggaat cctgggcacg tacgaatcgc gggccctttt ccgcccgtcg
     2401 acctggaccc ttcccgacac gctcgttacg accgtcatcg acacgatccg cttccgcgtg
     2461 gatttcgatc cccgcgtgga ccgctacctg accgggaacc gggttcttgc gctcaacagg
     2521 gcggcggcat gggacacgac gaacgtgggg tggcccggcc ctgccctggg cccgctgctc
     2581 gggaccagcc ccgacgccca ggcgcccttc acgatcgagc tcggagcttc cgcgatcagc
     2641 caggtccgag cctgggcggc ggacccgacg ttcccagggt tcgtcctgag catcgacagc
     2701 ggcaccggcg tgcgcggatt cctcgcgggg accggccgga tcgagatcgt ctaccacacg
     2761 actctcgcgc cgacgacgca cgtcaccaag gtgacgcgac tgcccaccga tctctcgatc
     2821 cagaccccgg ccgcgcccgc ctccggatcg gagagcacgc tcctcctggg cggcctcttc
     2881 cagtccgaag tgatcctccg cgctcccgtg gcgccccctc cggcgggatt ctcgatcaac
     2941 gcggcccgct tcgtggcgca tctgctgggc cccgtctcgc ccgacacggt ggagatcaag
     3001 gcctaccgca tccggaacgc atggaccgag ggggtctccg tcgacagcac cctggggctg
     3061 gacgccaagc cgctcgagac cttgaccggc gtccgcgtcg tcgcgggcga ttcgctcacg
     3121 atcccgatcc cgatctcgct cgcccgggaa tggtcggccg attccacgtc caacaagggg
     3181 atcctgatcc gcgtgaccaa cagcttctat gcctcggaga tcgagatcgg ctcgcgggag
     3241 tccgccacgc cgccggtgct ccgggtcaat accacgagcc cgccgcccgg gagattctga
     3301 gatgagccct ctccggaagt cctggatggc gcggcttcga cccgcggcgc ctttcctcgc
     3361 ggcggcgctg atcgcgatcc cggcatccgt ccgcgcctcg tcggcgtact cggcgggtgg
     3421 actcggcgag ccctcgctgg aagagccggc gcgcctgcgc gcgctgggcg gcgcgggcgc
     3481 ggccgagcac ggaccgcgcg cgttctccct cgtgaacccg gcttccgtcg ccgaggtgga
     3541 ccatctgatc ctcgagggaa ccgtgatgcc ctcgatgcgc cgcgtcagcg cgctctcctt
     3601 tccgggcgaa accgcgcgcg agacggtcgt cccgtcgctg cgagccctcg tccgggtccc
     3661 ggggcgcttc accctgggcg gcgcctatct cgtcggcacg aacggacgct tcgggatcga
     3721 ccgtccggag agctcgggcg cgccctccac gctgcgcatc gacggcgcgg gcggcatcga
     3781 cctgatccgg atcaccctcg cgcggcgact cagcccctcg gtcaacctgg gcctcgatta
     3841 cgaagtgatc gtgggcagct tccgcgagga ctgggcgcgc gatttcgtgg atccggcgct
     3901 gatcaccacg cgcgatacgc tcgagacgac gtgggagcgg caggggcggt ggcgtttcgg
     3961 cgcccaggtg caccgcggga cctggtcgct cggcggcgtc tacgagctgg cgcgccggct
     4021 gcccctggcg acgacgcagc gcaccgcggg caccttcgtg cggcaggagc accgcaatct
     4081 caccatcccc tcgggattcg cggtgggggg gatggcgccg atcaccgatc gcctgcgcgc
     4141 ggtggggcag taccggcgcg ccgattggag ccgggagtcc ctgttgagcg accgggtcga
     4201 cttccgcgcc gagcagcgct ggagcttcgg cctggagctg ctcccggcgg cggacgcgac
     4261 gcgtttcctg acccgcctgc cgctccgggt gggcgcctcc gttctcacct ggcccgatct
     4321 tttggtccgc gccgacgcga ccgacatctc cggcggcacg gcgggggtca aggaagtcgc
     4381 gttttccctg gggacgggac tccggagtca ggatcgaggc ggtgcggtgg atctctccct
     4441 ggaagcaggg tcgcgcgggg acaaggacgc gctcggggtc agcgagaagt tcctgcggct
     4501 ggcgatcagc ctacaggtca gcgacgacac ctggaagtga cgcgacccgg tcggcgaccc
     4561 gccgcgcgcg gcgcgccggg gccggctcgc cgcagcggtc gcaccgggcg aggagcctcg
     4621 cccgctctcc gtaatccagc accgtagtca cgaacgccgc cgtcgcgtcc agcgaggggc
     4681 agattccgtc ggcgcctccg ctcgcggggt cgagccgctc gggcaggagt cccgtgccct
     4741 cggcgcgcgc cgccgcccag agcagaagaa tccgcgcctc ctccaggtcc tgcgatttcc
     4801 gcgccacccg gatcatgtgc tgcgccagcc agagcgtcgg cgcgaccgag gggcggcccg
     4861 ccgcgcggtc gcgatcggga cccggccccg ccgcgtccgc ctcgtgccgc gcgagccccg
     4921 ccacgccgga gcgcacccac aaggcgccgc gcaccgccgc gcacgtggcg cggactcgcg
     4981 gatcctcggg ctcgaggtcg tcgaagagcc cgagccagag gagcgtcgcg tccagggtgg
     5041 cgtccagccg ccgctctccc tcttccatca ccaccgagcg caggaaccgc ccccgctccg
     5101 gatcgtagag cgcgcgcgag accgcgcggc cgagcatgtc ggaggcggcg agccacgcgc
     5161 gggcgcgggc cgtctcgccg aagagcgccg ccatgcgcga cgcggcccga agcccgccgc
     5221 gcaccgcagc ggccgtcgcg gcgtgagccc cccagcgctc gtccccccag gggttgctcc
     5281 aacggtcggc g
//
LOCUS       DASSLZ010000235         6040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_973775, whole genome shotgun sequence.
ACCESSION   DASSLZ010000235 DASSLZ010000000
VERSION     DASSLZ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973775"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VFM00_08830"
     CDS             complement(<1..506)
                     /locus_tag="VFM00_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498392.1"
                     /translation="MSWIFSRGGAGTRAGAGGAVVGCALGLVPAVRVLLGHPSESLRL
                     PWSLPLASFSLEIDALSAFFLTAIYGLSALAAVYGAGYGRVGAGRRGAAAWLAFNLLV
                     ASMALVVAARNGVLFLAAWEMMTIASWFLVTLRHDRAEARRAGRLYLVAAHVGTAFLL
                     VFFLGLAGS"
     gene            complement(691..2562)
                     /locus_tag="VFM00_08835"
     CDS             complement(691..2562)
                     /locus_tag="VFM00_08835"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7498393.1"
                     /translation="MRPRLTLFLAITVLAHAPSALAVPLPGLARPTEHSASLFDHDDH
                     MAVNQLDMLVSNHGSLAYDIATGNGGLVYPTGTVKTALFAAGIWIGAKVNGAVRVALG
                     EYAQEYWPGPMSGGSYQADQPAFHNFAFDAANQLSPGDLADYASQGGPMEGSGHPLLF
                     GDATIWSVFNDANPLVHHNTAGSTNPLGVEVQQTVCAYSGPAALGKTIFVKWKVTAKG
                     PDTLDSAYVSIWCDPDLGGAIDDLVGCDTTLALGYCYNATNADPVYGSSPPAVGITLI
                     RGAIVSPSVGVSDTLGMTSFDKYIGGTDPANAGESYAYMRGVRADGTPWHVLDDPLQP
                     ETRYPVSGLDPSLPSSATNWLDSNPADRRLMLSTGPFSMAPGESQEIIFAILVGQGAN
                     RFASISALKSMVPLVRPFAPAPSPGTSVGIVSVDVALIPETIDLASRAPWVDALIEPS
                     GARADAIDAGSVRLAGVAPDGGVAEVADHDANGRADLLLRFPRASLDPRLMPGAVSLD
                     VSGRLRTGDSFEGTATLRVVDGRHAPLAASLAPLPMRPAGNLRFVTTRAGSARVSLFD
                     ARGRRVRTLLMDRIGTGAHTVAIDGRDDHGRPLGSGLYWFRIEAAEGIATGRVVLLR"
     gene            2773..3471
                     /locus_tag="VFM00_08840"
     CDS             2773..3471
                     /locus_tag="VFM00_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7498394.1"
                     /translation="MPNTIGDRSEEKGFYFLTSSRLRWEPAACGPGEVRRLYVDPTDR
                     IEARLVRFPAGAVTAGAPELLGPEVYVVSGEFEADGERLREGDFHRTVGAAVDGRTEA
                     GCILFTLRECAPSAGTGEAPDVGSMCGADTIRVVERRWTDTGPGMRYKRLMRDPAHHI
                     EITLVQLDPGAAYPGHRYVGAEEIFILRGDCLCEGRKLGYGDYHRSVEGAVHRPAATD
                     GGCEFILVRHGMPD"
     gene            3564..4151
                     /locus_tag="VFM00_08845"
     CDS             3564..4151
                     /locus_tag="VFM00_08845"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7498395.1"
                     /translation="MSRPRSRSTPFAILGLLGFEPMSGYDIRKEVAASIGYFWSESYG
                     QIYPALRELSRDGLIRVKPGTPEGGRERRVYEITAKGRRTLAAWRAEPPRPAPVRNEL
                     LLKLFFGAPGGSGGEIAWLEALATKEAAQRREFRRIRRQLLEERRDHPSLPYWLAVVA
                     YGEHHSRAALRWARETSSALRGLGTARRKRGPLHE"
     gene            4144..4866
                     /locus_tag="VFM00_08850"
     CDS             4144..4866
                     /locus_tag="VFM00_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET7498396.1"
                     /translation="MNETLGANEAAPSDRLIAPVRHTVILVAILLAIAAYGVYGQHMA
                     AKASPADTRGSMLPLYLTLLVAEWALVRYVMVGLRKGATPFRELLGRRWTGWRDVLRD
                     VAIALAVFAIWTAGSSFVDQLTHAETPKAVENMLPRGGAEIAVWVLLSLSAGFCEEVV
                     YRGYLQRQLQALTGSGLAAVALQAVIFGISHGYQGARNVLFITVYGALFGALALWRRS
                     LKPGMILHAWTDIFGGIFQRSF"
     gene            complement(4882..5406)
                     /locus_tag="VFM00_08855"
     CDS             complement(4882..5406)
                     /locus_tag="VFM00_08855"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7498397.1"
                     /translation="MPPRVLLEPPRPGDEAALLQMNRASLEFHRGRAFTPTTHEHYAR
                     YLAACRQPNFLGLLVKRRADGAVVGAVELSQIVLGVFRSAYLGYYLGAAYAGQGLMSE
                     AIALTLNHAFTTMGLHRVEANIQPDNAASLALVAKLGFVREGYSRRYLKIRGRWRDHE
                     RWAILAEDWRIRRR"
     gene            complement(5452..5991)
                     /locus_tag="VFM00_08860"
     CDS             complement(5452..5991)
                     /locus_tag="VFM00_08860"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498398.1"
                     /translation="MKALVLALACVLLAPATVLAAPDVPPWGLNLDYLDRSGNPGDDF
                     YVYANGSWLKTATIPPDRPGSGVGLEMGLRNEDRMKAVIADLHARPNLTDEEKKLRDL
                     YDAFVDRKGIDAAGMKPAAGDLQRIAHADTPEAIVALMSDPSLRLGGRNDDGWYAAFP
                     DVGPGSKYYLPPEKRVHLW"
BASE COUNT          941 a         2047 c         2178 g          874 t
ORIGIN      
        1 gatccggcga ggccgaggaa gaagacgagc aggaacgccg tcccgacgtg cgcggcgacc
       61 aggtagaggc gccccgcgcg ccgcgcctcg gcccggtcat ggcgcagcgt cacgaggaac
      121 caggaggcga tcgtcatcat ctcccacgcc gccaggaaga gcacgccgtt ccgcgcggcg
      181 accaccagcg ccatgctcgc cacgaggagg ttgaacgcga gccaggcggc ggcgccgcgc
      241 cgccccgccc caacgcgtcc atagcctgcc ccgtagaccg ccgcgagcgc ggacaggccg
      301 tagatcgccg tgaggaagaa ggccgagagc gcgtcgatct ccaggctgaa ggacgcgagg
      361 ggaaggctcc aggggagacg gagcgattcc gaggggtggc cgagcagcac gcggacggcg
      421 ggaaccagtc cgagcgcgca tccgacgacc gctccgcccg cgccggcccg cgtcccggcc
      481 ccgccgcggg agaagatcca tgacaccagg cccgatagga caagcgttcc tacggacgcc
      541 agaaaccagg agatcggacg gccccttccc tggtgtggga cggctctcga atcgacgcgc
      601 accggaaaaa aggacaagcg gccgtcggga ccctacctca tgcggcggcg cgcggcaagg
      661 tccggggcgt cgcgaccgcg ccccacgtgc ctaacgcagg agcacgacgc gtcccgtggc
      721 gatgccctcg gcggcttcga tccggaacca gtagaggccg gagcccagtg gtcgtccgtg
      781 atcgtcgcgg ccgtcgatcg cgacggtgtg cgcgcccgtg ccgatccgat ccatgaggag
      841 cgtgcgcacc ctccttcccc gggcgtcgaa cagcgaaacg cgcgcggatc cggcccgggt
      901 cgtgacgaag cgcagattgc cggccggacg catgggtaac ggcgccagcg acgcggccag
      961 gggcgcatgc cgcccgtcga cgacgcgcag cgtggccgtc ccctcgaacg aatccccggt
     1021 tcggagccgt ccgctcacat cgagcgacac cgcgcccggc atgaggcgcg gatcgagaga
     1081 cgctcgcggg aagcggagca ggaggtcggc cctgccgttg gcgtcgtgat cggccacctc
     1141 ggccacgccg ccatcgggcg caacgccggc gaggcgcacg ctcccggcgt cgatcgcgtc
     1201 cgcgcgcgcg cccgagggct cgatcagcgc atccacccat ggagcgcggc tggcgagatc
     1261 gatcgtctcc ggaatcaggg ccacgtccac gctgacgatg ccgacggaag tgcccggcga
     1321 gggcgcggga gcgaacggcc ggaccagcgg gaccatgctc ttcagcgccg agatcgaagc
     1381 gaagcggttg gccccctggc cgacgaggat cgcgaagatg atctcctggg actcgccggg
     1441 cgccatcgag aagggccccg tggaaagcat caggcgccgg tcggcgggat tggaatcgag
     1501 ccagttcgtg gcgctgctcg gcaagctggg atccagtccc gagacggggt accgggtctc
     1561 cggctggagc ggatcatcga ggacgtgcca cggcgttcca tccgctcgga ctcctcgcat
     1621 gtacgcgtac gattcgccag cgttggcggg gtccgttcca ccgatgtact tgtcgaacga
     1681 ggtcatcccg agggtgtcgg acacgcccac ggacggcgat acgatcgccc cgcggatcaa
     1741 ggtgatcccc acggcgggcg ggctgcttcc gtaaacggga tcggcgttcg tcgcgttgta
     1801 gcagtagccc agagccagcg tcgtgtcgca ccccacgaga tcatcgatgg ccccgcccag
     1861 gtccggatcg caccagatcg acacgtaggc cgaatcgagc gtgtccggtc ccttcgcggt
     1921 caccttccat ttcacgaaga tcgtcttccc gagcgctgcc gggccgctgt aggcgcatac
     1981 ggtctgctgg acctcgacgc cgagagggtt ggtggacccg gcggtgttgt ggtgaacgag
     2041 cggattggcg tcgttgaaca cgctccagat cgtcgcgtcc ccgaagagga ggggatggcc
     2101 cgagccctcc atgggcccgc cctgcgacgc gtagtcggcc aggtctcccg gcgacagttg
     2161 attggccgcg tcgaaggcga agttgtgaaa cgcgggctgg tccgcttgat agctcccgcc
     2221 cgacatcggt ccgggccagt attcctgggc gtattcgccc agcgccacgc gaacggcgcc
     2281 gttgaccttg gcgccgatcc agatccccgc ggcgaacagg gccgtcttga ccgtgccggt
     2341 cgggtagacc agcccgccgt tgcccgtagc gatatcgtag gcgagggagc cgtggttgga
     2401 gaccaacatg tccagctggt tcacggccat gtggtcgtcg tgatcgaaga gagacgcgga
     2461 atgctccgta ggccgggcca gaccgggcaa cggaacggcg agggccgatg gcgcatgggc
     2521 caggaccgtg atggcgagga aaagcgtcag gcgagggcgc atcgagtatc tccggcggaa
     2581 cggtggaagc acgcggggag acagcttcga gtttgctccc gccggcgcgc gccgtccagc
     2641 cgtctccatc ccgaacggcg gatccagggg agcgggcgcc cccgcgccat cctactttgg
     2701 taccgcttgc gcgggccccc gcggcgggcg gaccctcatc ctcgaggtgg tcgagccgag
     2761 gaggggcgag ccatgccgaa cacgatcggt gatcgaagcg aagagaaggg cttctatttc
     2821 ctgacgtcca gccggctccg ctgggagccg gccgcatgcg ggccgggcga agtgcgccgg
     2881 ctgtacgtgg atcccacgga ccggatcgaa gcgcgcctgg tgcgctttcc cgccggggcg
     2941 gtgacggcgg gcgcgcccga gctgctcggc ccggaagtgt acgtcgtctc gggcgagttc
     3001 gaggccgacg gggagcgcct gagggagggc gatttccacc ggaccgtggg cgccgcggtc
     3061 gacggccgca ccgaggcggg ctgcatcctg ttcaccctgc gcgagtgcgc gccctcggcg
     3121 gggaccggcg aagccccgga cgtcggctcg atgtgcgggg ccgacacgat ccgcgtcgtg
     3181 gagcgccgct ggaccgacac gggacccggc atgcggtaca agcggctgat gcgcgacccg
     3241 gcccaccaca tcgagatcac gctggtgcag ctggatccgg gcgccgccta ccccggccat
     3301 cgctatgtcg gcgccgagga gatcttcatc ctgcggggcg actgcctctg cgagggtcga
     3361 aagctgggtt acggcgatta ccaccggtcg gtggagggcg cggtgcaccg gcccgccgcc
     3421 accgacggag gctgcgagtt catcctggtt cgccacggta tgcccgacta gcgcgcgcgg
     3481 cgtccgtggc aggaggggcc ccgttccggg gcccttttcg tttgggggtg gacgcccgct
     3541 cggccgtgat atatctttta gatatgtcac gaccccgatc gcgctccacc cccttcgcca
     3601 tcctcggcct cctcggattc gagccgatgt cgggctacga catccggaag gaggtcgccg
     3661 cgagcatcgg ctacttctgg agcgagagtt acggacagat ctatccggcc ctgcgggagc
     3721 tctcccggga cggactgatc cgggtgaagc cgggaacgcc cgaagggggc cgcgagcgcc
     3781 gcgtctacga gatcacggcg aagggccgcc ggacgctcgc ggcctggagg gcggagccgc
     3841 cgcggccggc gcccgttcga aacgagctcc ttctcaagct gttcttcggg gctcccggcg
     3901 gcagcggcgg cgagatcgcg tggctcgaag cgctcgcgac gaaggaagcc gcgcaacggc
     3961 gcgaattccg acgcatccgc cggcagctcc tggaggagcg tcgcgatcat cccagccttc
     4021 cctactggct cgccgtggtg gcttacggcg agcatcactc ccgggccgcc ctccgctggg
     4081 cgcgcgagac gtcgagcgcg ctccgcggcc tcggtaccgc gcgccggaaa cgggggcctc
     4141 tccatgaatg aaaccctggg ggcgaacgaa gcggcccctt cggaccggct gatcgcgccg
     4201 gtccgccaca ccgtcatcct ggtcgcgatc ctgctcgcga tcgcagcata cggcgtctac
     4261 gggcagcaca tggccgccaa ggcatcgccg gccgacaccc gcgggtcgat gctgccgctc
     4321 tacctgaccc tactcgtcgc cgagtgggcc ctggtccgct acgtcatggt gggtcttcgg
     4381 aagggagcga cgccgttccg cgagctcctg ggacggcggt ggaccggatg gagagacgtg
     4441 ttgcgggacg tcgcgatcgc gctcgcggtc ttcgcgatct ggaccgcggg ctccagcttc
     4501 gtggatcagc tcacccacgc cgagaccccg aaggccgtcg agaacatgct gcctcgcggg
     4561 ggtgccgaga tcgccgtctg ggttctgctc tcgctgtccg cgggcttctg cgaagaggtg
     4621 gtctaccgcg gctacctcca gcgccagctt caggccctga ccgggagcgg cctcgccgcg
     4681 gtggccctcc aggccgtgat cttcggcatc tcgcacgggt accagggcgc gcggaacgtg
     4741 ctcttcatca cggtctacgg cgcgctcttc ggcgcgctcg ccctctggcg gcggagcctc
     4801 aagcccggga tgatcctgca cgcgtggacg gacatcttcg gagggatctt ccagaggagc
     4861 ttctagcccg cggagggcga cctatctccg gcggatccgc cagtcctcgg ccaggatcgc
     4921 ccaccgctcg tgatcgcgcc agcggccgcg gatcttgagg tagcggcgcg agtacccttc
     4981 ccgcacgaaa cccagcttcg cgaccagcgc gagcgaggcc gcgttgtccg gctggatgtt
     5041 ggcctccacg cggtgtaggc ccatcgtcgt gaacgcgtgg ttcagcgtga gcgcgatcgc
     5101 ctcgctcatg agcccctgcc ccgcgtaggc cgcgccgagg tagtagccga gatacgcgct
     5161 gcgaaagacg cccagcacga tctggctcag ctcgaccgcg cccaccacgg cgccgtcggc
     5221 gcgccgcttg accaggaggc ccaggaaatt cggctggcgg caggcggcga gatagcgtgc
     5281 gtagtgctcg tgcgtggtcg gggtgaaggc gcgcccccgg tggaactcga ggctcgcgcg
     5341 gttcatctgc aggagcgcgg cttcgtcccc ggggcgcggc ggctccagga gtacgcgtgg
     5401 cggcacgatc agcggctcgg gacgttcagg gaatcgagac ccgggaggcg ctcaccagag
     5461 gtggacgcgc ttctccggag gaaggtagta cttggagccg ggcccgacgt cggggaaggc
     5521 ggcgtaccat ccatcgtcgt ttcgcccgcc cagccggagc gagggatcgc tcatcagcgc
     5581 gacgatcgct tcgggcgtgt ccgcgtgcgc gatgcgctgg aggtcgccgg ccgcgggctt
     5641 catgccggcc gcgtcgatcc ccttgcggtc cacgaacgcg tcgtagaggt cgcggagctt
     5701 cttctcctcg tccgtgaggt tcggccgcgc gtgcaggtcg gcgatgacgg ccttcatccg
     5761 gtcttcgttc ctgaggccca tctccagccc cacgccggac ccggggcgat ccggcggaat
     5821 ggtggcggtc ttgagccagc tgccgttggc gtacacgtag aagtcgtcgc ccggattgcc
     5881 ggagcggtcc aggtaatcca gattcagtcc ccagggaggc acgtcgggag cggcgaggac
     5941 tgtcgcgggc gcgagcagga cgcaggcaag ggcgagaacg agggctttca cggcgggtct
     6001 ccgggatccg aggacgctgg gaccgggatc ggatgacgaa
//
LOCUS       DASSLZ010000236         4487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1345735, whole genome shotgun sequence.
ACCESSION   DASSLZ010000236 DASSLZ010000000
VERSION     DASSLZ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345735"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFM00_08865"
     CDS             complement(<1..345)
                     /locus_tag="VFM00_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498399.1"
                     /translation="MGFIRRFAYLVIALILALVFIWGRAEREHKRADEALRQARAALD
                     SAKAMRAGGTGERGVFLSADEIAALRRAGLTDPVNQLRSDLEAHGSLIPVKGVLGGKM
                     GFYDRDGVVLLPG"
     gene            complement(385..3609)
                     /locus_tag="VFM00_08870"
     CDS             complement(385..3609)
                     /locus_tag="VFM00_08870"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET7498400.1"
                     /translation="MISRSSLLALLCGALLVCGPIVAPTRAALAPDDDDDTTTGAPSE
                     ASEPPPLSPASADTLPHPDPARTRAFHDAVYALAADSMEGRGIGTAGIAKAATWIERR
                     MKAIKLEPAFAGSYRQRFPIKIGVTLKAGNRIEGLDSTDWTPLGFSSSGDFSGPLVFC
                     GYGIDAPAIGYQEFEGIDLKGKVALMLRYEPQEKDDGSPFDGKRPSRWSALRYKAMQA
                     RERGATAVLFVTGPLQDEGTDKVPALRNDGPESPAGLPVAQVKLSAASRWLAKAGIDL
                     LAFQKDVDRDLTPRSRVIGAVTIHGKIAVEDKYVDSDNLAGIIPGKGRLKNEYVVVGA
                     HYDHLGWGGESSMRPNDHAIHNGADDNASGDAAVLVAADGLRKDLARQVSHRTVLVCL
                     FSGEEVGLAGSAWLVDHPPIPVDRIAAMVNLDMVGRLRGNTLIALGLESAPEWAPLLR
                     DAAVDTKLDVAARGDGYGPSDQTSFYAKGIPVIHLFTGTHEQYHTPDDKPATINAEGG
                     GIVARFTQVLVERAAVRDKRLTYARSNAAPTMTGDSRGYGAYLGTVPDFKAMESANGG
                     VLLADVRGGGPAEIAGIKGGDLVVSMAGTRIENLYDMTYALQDHRPGQTVEVTVVRGG
                     DTLTFRVTLGDRARMGMAGPAVAKAEAPKMPQDVSHVAHASGEPADSGAVTVAAVGPG
                     AEVGPSAAPSPAPAAPGPAPAAPPMPGNPHAGMGGGPTGPPGAEIGAAAVDSFYLGRP
                     GDDFVVKAGKPYGPAAPGERHLSDIRQLTFGGENAEAYFSPDGKKLIMQSTPRGAKCD
                     QEYVLDLSTGDMKRVSSGKGRTTCGYFDYPEADRIVYSSTQGASDSCPPSPDMSQGYV
                     WAIYDGYDIWEANPDGSNAKNLTNSPGYDAEATWCHRGGKLVFTSTRDGDLELYLMDE
                     AGQVKRLTHTPGYDGGAFFSPDCSQIVFRASRPTGEKLTDYQNLLKKGLIRPGELEIY
                     VMRADGTGVKQLTQNGAANFCPTFYPDGKRIIWASNSGAAGPREFDLWLLDTRGGKAE
                     QVTTAPGFDGFPHFSPDGRFLVWASNRADVASHETNIFIARWKD"
     gene            3753..4298
                     /locus_tag="VFM00_08875"
     CDS             3753..4298
                     /locus_tag="VFM00_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7498401.1"
                     /translation="MTLSFRLGLIGLLALGTALLPANRTLAGDDSSAVSATAAAAPAP
                     AAGAPAANAKDTTPTKADLVQPAEVAKDLALPEWRRPVLLHVGFSVLYKSGHIPGSKY
                     VGSGSTARGRAELKKALKAIPKDKAVVLYCGCCPWEHCPNVNPAFRVARGLGYKNVKI
                     LYIVQDLQHDWVDKGYPALES"
BASE COUNT          642 a         1572 c         1576 g          697 t
ORIGIN      
        1 tcccggcagc agcaccacgc cgtcgcgatc gtagaacccc atcttcccgc ccaggacgcc
       61 cttcacggga atcagcgagc cgtgcgcttc gaggtcggag cggagctgat tcacgggatc
      121 ggtcaggccg gcgcggcgga gcgccgcgat ctcgtcggcc gataagaaca cgccgcgctc
      181 gccggtgcct cccgcacgca tcgccttcgc cgaatcgagc gccgcgcgcg cctgcctcag
      241 cgcctcatcg gcgcgcttgt gctcccgctc ggcgcgtccc cagatgaaga cgagcgcgag
      301 gatgagggcg atgacgaggt aggcgaaccg tcgaatgaag cccatggcta gatccctgcc
      361 gaccgcggcc gaacgtccgc ggaactagtc cttccagcgc gcgatgaaga tgttcgtttc
      421 gtgcgaggcg acgtcggcgc ggttggaggc ccagaccagg aagcggccgt ccgggctgaa
      481 gtgggggaag ccgtcgaagc ccggagccgt ggtgacctgc tccgccttgc cgccgcgcgt
      541 gtcgagcagc cagagatcga actcgcgcgg tcccgcggcg ccggagttcg aggcccagat
      601 gatccgcttc ccgtcgggat agaacgtggg gcagaaattc gcggcgccgt tctgggtcag
      661 ctgcttcacc ccggtgccgt cggcccgcat gacgtagatc tccagctcgc ccgggcggat
      721 cagccccttc ttcagcaaat tctgatagtc ggtgagcttc tctccggtcg gccggctggc
      781 gcggaagacg atctgcgagc agtcggggct gaagaaggcg ccgccgtcgt agcccggcgt
      841 gtgcgtcagc cgcttcacct gccccgcctc gtccatcagg tagagctcca ggtcgccgtc
      901 gcgcgtcgag gtgaagacca gcttcccgcc gcggtggcac caggtggcct cggcgtcgta
      961 gccgggcgag ttcgtcaggt tcttcgcgtt cgacccgtcc ggattcgctt cccagatgtc
     1021 gtagccatcg tagatcgccc atacgtagcc ctgcgacatg tcgggcgagg gcgggcagga
     1081 atcggacgcg ccctgcgtcg aggagtagac gatccggtcg gcctcggggt agtcgaagta
     1141 gccgcacgtc gtgcgcccct tgccgctcga cacgcgcttc atgtccccgg tcgaaaggtc
     1201 cagcacgtac tcctggtcgc acttcgcgcc gcgcggcgtc gattgcatga tcagcttctt
     1261 tccgtcgggg ctgaagtacg cctcggcgtt ctcgccgccg aacgtgagct ggcggatgtc
     1321 gctcaggtgg cgctctcccg gggccgcggg gccgtacggc ttccccgcct tcaccacgaa
     1381 gtcatcgccc gggcgtccga gatagaagga gtccaccgcc gccgcgccga tctccgcacc
     1441 gggcgggccc gtcgggcccc cgcccattcc ggcgtgcggg ttgccgggca tgggaggcgc
     1501 cgccggcgcg gggccaggcg ccgccggcgc ggggctcgga gccgcggagg gaccgacctc
     1561 ggcccccggg cccacggcgg ccacggtcac ggcaccgctg tcggccggct cgccgctcgc
     1621 gtgcgcgacg tgggacacgt cctgcggcat cttgggcgct tccgccttgg ccaccgcggg
     1681 gccggccatc cccatccggg cccggtcgcc cagcgtgacg cggaaggtca aagtgtcgcc
     1741 gccgcgcacg accgtcacct cgaccgtctg tccggggcga tggtcttgaa gggcgtaggt
     1801 catgtcgtag aggttctcga tgcgcgtgcc cgccatggag acgacgaggt cgcctccctt
     1861 gatgcccgcg atctcggcgg gtccgccccc gcgcacgtcg gcgagcagca cgcccccgtt
     1921 cgcgctctcc atcgccttga agtcgggcac ggtgccgaga taggcgccgt agccgcggct
     1981 gtcgcccgtc atcgtcggcg ccgcgttgga ccgcgcgtag gtgagccgct tgtcgcgcac
     2041 ggccgcgcgc tcgacgagga cctgcgtgaa gcgcgcgacg atgcccccgc cctcggcgtt
     2101 gatcgtcgcg ggcttgtcgt cgggcgtgtg gtactgctcg tgcgtgccgg tgaagaggtg
     2161 gatcaccggg atgcccttgg cgtagaagct ggtctgatcg gaggggccat agccgtcgcc
     2221 gcgcgccgcc acgtccagct tggtgtcgac cgcagcgtcg cgaaggagcg gcgcccactc
     2281 cggcgccgac tccaggccca gcgcgatcag cgtattcccg cgcaggcggc ccaccatgtc
     2341 cagattcacc atcgccgcga tccggtcgac cgggatgggg ggatggtcca ccagccaggc
     2401 cgacccggcc aggcccacct cctcgcccga gaagaggcag acgaggacgg tgcgatggct
     2461 gacctggcgc gcgaggtcct tgcgaaggcc gtcggcggcg acgagcacgg ccgcgtcgcc
     2521 cgaggcgttg tcgtcggcgc cattgtggat cgcgtggtcg ttggggcgca tcgagctctc
     2581 gccgccccag ccgagatggt cgtagtgcgc gccgaccacc acgtactcgt tcttgaggcg
     2641 ccccttcccg gggatgatcc cggcgaggtt gtcggagtcg acgtacttgt cctcgaccgc
     2701 gatcttcccg tggatcgtga ccgcgccgat cacgcgcgac cggggcgtga ggtcgcggtc
     2761 cacgtccttc tggaacgcga gcaggtcgat tccggccttg gccagccagc gcgacgccgc
     2821 gctcagcttg acctgcgcga cgggaagccc cgcggggctc tcgggtccgt cgttccgaag
     2881 cgccgggacc ttgtcggtgc cctcgtcctg gagcgggccg gtcacgaaga gcacggcggt
     2941 cgcgccgcgc tcccgcgcct gcatcgcctt gtagcggagc gccgaccagc ggctggggcg
     3001 cttgccgtcg aagggagagc cgtcgtcctt ctcctgtggc tcgtagcgga gcatcagcgc
     3061 gaccttcccc ttcaagtcga tcccttcgaa ctcctggtac ccgatcgccg gcgcgtcgat
     3121 gccgtagccg cagaacacca gcggtcccga gaagtcgccg ctggaggaga acccgagcgg
     3181 cgtccagtcc gtggagtcga gcccctcgat gcggttgccg gccttgagcg tcaccccgat
     3241 cttgatcggg aagcgctggc gataagagcc ggcgaacgcg ggctcgagct tgatcgcctt
     3301 catgcgccgc tcgatccagg tcgccgcctt ggcgattccg gcggtcccga tcccgcggcc
     3361 ttccatggaa tcggcggcga gggcgtagac ggcgtcgtgg aacgctcgcg ttcgcgccgg
     3421 atcgggatgg ggaagggtgt cggcggaagc gggcgagagc ggcggcggct cggaagcctc
     3481 cgacggcgcg cctgtggtcg tatcgtcgtc gtcgtccggc gccagggcgg cgcgcgtggg
     3541 cgcgacgatg gggccgcaga cgaggagggc gccgcacaga agtgccagga gcgatgaccg
     3601 ggagatcacg tcctcatcct agcatccccg gcgcccaccg cgctctcgac atctcgcgcg
     3661 cgccgggtgc agactcctgg ggaatcgcca tcgcgccgca cggtaccaaa gtcgcattgt
     3721 gcggctctct cattccgcga aaggctcatc ccatgacgct cagttttcgg ctcggtctga
     3781 tcggacttct ggccctgggc acagctctcc tcccggcgaa ccgcacgctc gcgggggacg
     3841 actcctcggc ggtctcggcc actgccgctg cggcacctgc accggcggcc ggcgcccccg
     3901 cggcgaacgc caaggacacc acgcccacca aggcggacct ggtccagccg gcggaggtgg
     3961 ccaaggacct ggcgctgccc gagtggcggc gccccgtgct gctccacgtc ggcttcagcg
     4021 tgctctacaa gagcgggcac atcccgggtt cgaagtacgt ggggtcgggc tcgaccgcgc
     4081 gggggcgtgc cgagctgaag aaggcgctga aggcgatccc caaggacaag gcggtggtcc
     4141 tctactgcgg ctgctgcccg tgggagcact gccccaatgt gaatcccgcc ttccgcgtgg
     4201 cgcgggggct gggctacaag aacgtgaaga tcctctacat cgttcaggac ctgcagcacg
     4261 actgggtcga caagggatat cccgcgctgg agtcctgagc gcgcgtcagc gcttcaccag
     4321 ggaccggaac catcggacga tgctcgaacc gagcacctgt gccgagagtc agtgtccgag
     4381 cgccgccgcc gcggtcatgg ccgagccaac cgtgaccatg gtgaccgcga ccaaggcgtc
     4441 gcggcgcagg aagaaccccg tgacgacgaa tcccgtgccg atcagca
//
LOCUS       DASSLZ010000237         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1087505, whole genome shotgun sequence.
ACCESSION   DASSLZ010000237 DASSLZ010000000
VERSION     DASSLZ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087505"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VFM00_08880"
     CDS             <1..861
                     /locus_tag="VFM00_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014712155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7498402.1"
                     /translation="FLELFTTVIVAYIFVLLGVVHERVALTLIYLDFILYSIGGVIGT
                     MHHMYFNGTPVEAMALGATFSAAEVVPLTFLTVEAWSFLQLGAVQVGEGKRAFPHRWA
                     VMFLAAVGFWNFLGAGIFGFLINLPIVSYYEIGTALTANHGHTAFMGVYGMLAIGIAL
                     FCLRYLMPADRWSDRAAKFSFWSLNIGLAWMSFATLFPLGVLQLYHSVKAGYVEARSL
                     EFLTSGMNVWIEWVRLPGDLLFIAGTVPILWLAWNAVRGARRIPESIGEAAPLFMDAA
                     TRTDRAGAES"
     gene            858..1397
                     /locus_tag="VFM00_08885"
     CDS             858..1397
                     /locus_tag="VFM00_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498403.1"
                     /translation="MTSFAGLTPLQEAAFVGGYALFLVGVAAALGWAARRSHARVLRV
                     KAIGFRYHQAFDAWQCTEGTYLWRIERDEKARLVRYRAKAEMCNACPLKNACTDSNDG
                     RELLRPMDAWPHTEMARFQGAISLALILLAGVLVAVEAGRQADPRLQVTFGGAALPCF
                     FLVRREIARLRAVRTALPR"
     gene            1493..2395
                     /locus_tag="VFM00_08890"
     CDS             1493..2395
                     /locus_tag="VFM00_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498404.1"
                     /translation="MAAPPPATSFESWWQDGRAELAGYRYHVTRYGQRRTGRCAMITV
                     TEPFSAAKRVKVDDPSKNPADTFEALKLNLSRSFQTGIYDYHTMISLFTRSRDFAPVK
                     VAFSATEWCGQVYEEQAFGPRAISGFVRSYFEDESRALNLPAKPGGIAEDDLFLVLRG
                     LRGDFLPPGRSRSLPLLASPFITRLAHRAPDWVVANVARAREPVQVHVPAGSFPAILY
                     TAALDDGRTGRFWIEAAWPHRVLKWTWTATAAPRGRMGRDGLDEGELIGSTRLPYWQL
                     NQPGGERYLRELGLAPLTPSVTGR"
BASE COUNT          313 a          927 c          847 g          417 t
ORIGIN      
        1 ttcctcgagc tcttcaccac ggtgatcgtg gcctacatct tcgtcctcct gggcgtcgtg
       61 cacgagcggg tcgcgctcac gctgatctac ctggacttca tcctctactc catcggcggc
      121 gtgatcggga cgatgcacca catgtacttc aacggcacgc cggtcgaggc gatggcgctg
      181 ggcgccacct tctccgcggc cgaggtggtg ccgctcacgt tcctgacggt cgaggcatgg
      241 agcttcctgc agctcggcgc ggtccaggtg ggcgagggga agcgggcgtt cccccatcgc
      301 tgggcggtca tgttcctcgc ggcggtcggc ttctggaact tcctcggcgc cgggatcttc
      361 ggcttcctga tcaacctccc gatcgtctcc tactacgaga tcggcaccgc gctcacggcg
      421 aaccatgggc acaccgcgtt catgggcgtc tacggcatgc tcgcgatcgg gatcgcgctc
      481 ttctgcctgc gctatctcat gcccgccgac cgctggtcgg accgggcggc caagttctcg
      541 ttctggtcgc tcaacatcgg gctcgcgtgg atgtccttcg cgaccctctt cccgctcggc
      601 gtgctgcagc tctaccactc ggtcaaggcg ggctacgtcg aggcgcgctc gctcgagttc
      661 ctcacctccg gcatgaacgt ctggatcgag tgggtgcggc tgccgggtga cctcctcttc
      721 atcgcgggca ccgtccccat cctctggctc gcctggaacg cggtgcgcgg cgcgcggcgg
      781 atcccggagt cgatcggcga ggcggcgccg ctcttcatgg acgcggccac gcgcaccgac
      841 cgggccggag ccgagtcgtg acctcgttcg cgggcctgac gccgctccag gaagccgcct
      901 tcgtcggcgg ctacgccctg ttcctcgtgg gcgtggccgc cgcgctggga tgggcggcgc
      961 gccggtcgca cgcccgcgtg ctgcgcgtga aggcgatcgg cttccgctat catcaggcct
     1021 tcgacgcgtg gcagtgcacg gaggggacct acctgtggcg gatcgagcgg gacgagaagg
     1081 cgcggctggt ccgttaccgg gccaaggcgg agatgtgcaa cgcctgcccg ctcaagaacg
     1141 cctgcaccga ctcgaacgac ggccgggagc tcctgcggcc gatggacgcc tggccgcaca
     1201 ccgagatggc gcgcttccag ggagcgatct cgctcgccct gatcctgctc gcgggggtgc
     1261 tcgtcgcggt ggaagcgggg cggcaggccg acccgcgcct ccaggtgacg ttcggcggcg
     1321 ccgccctccc ctgcttcttc ctcgtgcgtc gcgagatcgc gcggcttcgg gcggtgcgca
     1381 ccgcgctccc gcgctgaagc gcgccgcgct cgtcgcgttg ctggcgtgcg ggctcgtcgt
     1441 ggccgttgcc agcgtggtag gggctgcccc gccacgcccc gcccagcggg cgatggctgc
     1501 gccaccgccc gcgacatcct tcgaatcctg gtggcaggac ggacgggccg agctggccgg
     1561 ctaccgctac cacgtcacgc gctacggcca gcgccggacg ggacgctgcg ccatgatcac
     1621 cgtcaccgag cccttcagcg cggcgaagcg cgtcaaggtg gacgatccct cgaagaaccc
     1681 cgccgacacc ttcgaggcgc tcaagctgaa cctgtcgcgg agcttccaga ccgggatcta
     1741 cgactatcac acgatgatct ccctcttcac gcgaagccgc gacttcgccc cggtcaaggt
     1801 ggcgttcagc gccaccgaat ggtgcggcca ggtctacgag gagcaggcct tcggcccccg
     1861 cgcgatcagc gggttcgtgc gctcctactt cgaggacgaa tcgcgcgcgc tgaacctgcc
     1921 ggcgaagccg ggcggcatcg ccgaggacga cctcttcctg gtgctccgcg ggctgcgcgg
     1981 cgacttcctt ccgcccgggc gctcgcgctc ccttcccctg ctcgcgagcc ccttcatcac
     2041 gcgcctcgcc caccgcgcgc ccgactgggt cgtcgcgaac gtcgcgcgcg cgcgcgagcc
     2101 ggtccaggtc cacgtccccg ccggctcgtt cccggcgatc ctctacaccg cggcgctgga
     2161 cgacgggcgc accggccggt tctggatcga ggcggcctgg ccgcaccgcg tcctcaagtg
     2221 gacgtggacc gcgaccgcgg cgccgcgcgg ccgcatggga cgcgacgggc tggacgaggg
     2281 ggagctgatc ggctccacgc ggcttcccta ctggcagctg aaccagccgg gcggcgagcg
     2341 ctacctccgg gagctgggcc tcgcgccatt gacgccctcc gtcaccgggc gataggcttt
     2401 tccggacagc ggttccgccg ccgcgtcccg tgcggcgcgc gtctccaccg ggagccctcc
     2461 acgtgcccaa ggccctcctc cgtcatgtga atctcggatt cggc
//
LOCUS       DASSLZ010000238         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1228746, whole genome shotgun sequence.
ACCESSION   DASSLZ010000238 DASSLZ010000000
VERSION     DASSLZ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228746"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFM00_08895"
     CDS             <1..316
                     /locus_tag="VFM00_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HET7498405.1"
                     /translation="SPRWSPKGDRITYAARHDGIFDVVVMDANGQRPFQITSGAGHNE
                     NPQWSADGRKIYFSSTRSGRRQVYMMNADGSDVVQLTRGDECFNPAAGPRPKRPGVSR
                     AG"
     gene            401..931
                     /gene="pal"
                     /locus_tag="VFM00_08900"
     CDS             401..931
                     /gene="pal"
                     /locus_tag="VFM00_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HET7498406.1"
                     /translation="MSRSSRTPVLLLTALLAMILMAPGCSSRKKVSGGAEPPPPPSTS
                     DNTPPTETPPPPPTTGGGEETTASNLRDAYFDFDDAGLREDAKAALDGNAKYMESHAS
                     MNVVIEGHCDERGSVEYNLALGEKRARAAKDYLVAYGITATRMTTISYGKERPFDTGH
                     TEEAWAQNRRAHFVSK"
     gene            928..1698
                     /gene="ybgF"
                     /locus_tag="VFM00_08905"
     CDS             928..1698
                     /gene="ybgF"
                     /locus_tag="VFM00_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HET7498407.1"
                     /translation="MRTPASRHAAPLALALLLAGAAAGCAPGNYYRETQAQLDSLLTT
                     QADLVRRVDRLDRKLEETRGGVSSTRASTETNLAKLSERLDVVVSQLERNQERMQDFG
                     LKIDTVRQRMNTADSVRVAQGMAPRDTSGILDPEQAYQAAYSDYAAGRYKLAGEAFRE
                     FLRHYPNTEVSDNAQYWIGESLYAQGDFPSAIIEFRAVVDNYPKGDKVPAALLKIGIA
                     NARLNNTAEAKKSFDQVIRKYPRSPEAALAKERLAQLR"
     gene            1744..>3194
                     /locus_tag="VFM00_08910"
     CDS             1744..>3194
                     /locus_tag="VFM00_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET7498408.1"
                     /translation="MRFDKLTTKSQEALADAESRADRANHPEVRPEHLLEALLAQKEG
                     AVPAVIEAAHVDRAGLDRSLTRALASLPTVSGSRTIVLSTTLGRVLEQSEKEAEQLQD
                     EYVSTEHLLLAMLDPSIASPAGEALKQAGATREGIYHALLSVRGGEQVTTPDPEDKYQ
                     ALAKYARDLTETARKGKLDPVIGRDEEIRRVVQVLSRRTKNNPVLIGEPGVGKTAIAE
                     GLAQRIVSGDVPEGLKNRRLLALDLGALLAGAQFRGQFEERLKSVLKEVQRSEGQVIL
                     FIDELHTLVGAGAAEGALDASNLLKPALARGELHCIGATTLDEYRKRIEKDAALERRF
                     QPVFVGEPTVEQTIAILRGLRERYETFHKVKIQDAALVAAAVLSDRYIADRQLPDKAI
                     DLVDEAASRLRIEIDSLPAEIDTLERKRRQLEIERLALKREKDEGSKARRRAIEEEME
                     ETGAELARLRAHWEKERARIQKINEIKGRIEELR"
BASE COUNT          577 a         1107 c         1101 g          409 t
ORIGIN      
        1 ctcgccgcgc tggtccccca agggggaccg gatcacctac gccgcgcgcc acgacggcat
       61 cttcgacgtg gtggtgatgg acgcgaacgg ccagcgcccc ttccagatca cctccggggc
      121 cgggcacaac gagaaccccc agtggtctgc ggatggccgg aagatctact tctcgagcac
      181 gcgctcgggg cgccggcagg tctacatgat gaacgcggac gggagcgacg tggtccagct
      241 gacccgcggc gacgagtgct tcaaccccgc ggccggaccc cgtcccaagc gtccgggcgt
      301 gagccgggcg ggttgacgct cccgaccccc tgtgctaggt tgggcttcag actggaagtc
      361 gttgcggcat aatgaacttg aacccaacgg aggtccgcat atgagccgat cctctcgcac
      421 gccggtgctc ctgctgacgg cgctgctcgc gatgatcctg atggcgcccg gatgctcctc
      481 gcggaagaag gtttcgggtg gcgccgagcc gcctcccccg cccagcacgt ccgacaacac
      541 gcccccgacc gagaccccgc cacccccgcc gacgaccggt ggaggggagg agacgaccgc
      601 gtcgaatctc cgcgacgcct acttcgactt cgacgacgcc gggctccgtg aggacgccaa
      661 ggcggccctg gacgggaacg cgaaatacat ggagtcgcac gccagcatga acgtcgtgat
      721 cgaggggcac tgcgacgagc gcgggtcggt ggaatacaac ctggccctgg gcgagaagcg
      781 ggcgcgcgcc gccaaggact acctcgtcgc ctacggcatc acggccacgc gcatgacgac
      841 gatctcctac ggcaaggagc ggccgttcga caccgggcac acggaagagg cctgggccca
      901 gaaccgcagg gcccacttcg tctccaagtg agaacgcccg cttccaggca cgccgcgccg
      961 ctcgccctcg cgctccttct cgcgggggcc gcggccggct gcgcgcccgg gaactactac
     1021 cgcgagaccc aggcccagct ggacagcctc ctcacgacgc aggccgacct ggtccgccgc
     1081 gtcgataggc tggaccggaa gctggaggag acgcgcggcg gcgtgtcctc gacccgcgcg
     1141 tccaccgaga ccaacctggc caagctgagc gagcgcctcg acgtcgtggt gagccagctg
     1201 gagcggaatc aggaacggat gcaggacttc ggactgaaga tcgacacggt ccgccagcgg
     1261 atgaacacgg ccgacagcgt tcgggtcgcg cagggcatgg cgccgcgcga caccagcggg
     1321 atcctcgatc ccgagcaggc ctaccaggcc gcctactccg actacgcggc gggccgctac
     1381 aagctggcgg gggaggcgtt ccgcgaattc ctgcgccact accccaacac cgaggtctcc
     1441 gacaacgcgc agtactggat cggcgagtcg ttgtacgctc agggagattt tccgtcggcg
     1501 atcatcgaat tccgcgcggt ggtggacaac taccccaagg gggacaaggt cccggccgcg
     1561 ctcctcaaga tcgggatcgc gaacgcgcgg ctcaacaaca ccgccgaagc caagaagagc
     1621 ttcgatcagg tcatccggaa gtatccgaga agtcccgagg ccgcgctggc caaggagcgc
     1681 ctggcccagc ttcgctagcg ctgaacgacc gattctcccg ggccgcaatg aagggtcgcg
     1741 ggcatgcgtt tcgataagct gaccaccaag agccaggagg ccctcgccga cgccgagtcg
     1801 cgggccgacc gggcgaacca ccctgaagtg cgccccgagc acctgctcga agcgctcctc
     1861 gcccagaagg aaggcgcggt cccggcggtg atcgaggccg cgcacgtcga ccgcgcgggc
     1921 ctggaccgct cgctcacgcg ggcgctggct tcgctcccca ccgtatcggg ttcccgcacc
     1981 atcgtgctgt ccaccacgct cggccgcgtg ctcgaacagt ccgagaagga agccgaacag
     2041 ctgcaggacg agtacgtgag cacggaacac ctgctgctcg cgatgctcga cccctcgatc
     2101 gcttcgcccg cgggcgaggc gctgaagcag gcgggcgcga cgcgcgaggg gatctaccac
     2161 gcgctcctct ccgtccgcgg cggcgagcag gtcaccacgc ccgatcccga ggacaagtac
     2221 caggcgctgg cgaagtacgc gcgcgatctg accgagaccg cgcggaaggg gaagctggac
     2281 ccggtgatcg ggcgcgacga ggagatccgc cgcgtcgtgc aggtgctctc ccggcgcacc
     2341 aagaacaatc cggtcctgat cggggagccg ggtgtgggga agacggccat cgccgagggc
     2401 ctcgcgcagc ggatcgtgag cggcgacgtg cccgagggat tgaagaaccg ccggctcttg
     2461 gccctcgacc tgggcgccct cctcgccggc gcgcagttcc gcggccagtt cgaggagcgg
     2521 ctcaagtcgg tgctcaagga ggtgcagcgc tccgaggggc aggtgatcct gttcatcgac
     2581 gagctccaca cgctggtggg cgcgggggcc gcggaggggg cgctggacgc ctcgaatctc
     2641 ctgaagcccg cgctcgcgcg cggcgagctc cactgcatcg gcgcgaccac cctcgacgaa
     2701 taccggaagc ggatcgagaa ggacgccgcc ctggagcggc gcttccagcc ggtcttcgtc
     2761 ggcgagccga ccgtggagca gacgatcgcg atcctgcgcg gcttgcgcga gcgctacgag
     2821 acgttccaca aggtgaagat ccaggacgcg gcgctggtcg ccgcggcggt gctctccgac
     2881 cgctatatcg cagaccggca gcttcccgac aaggcgatcg acctggtgga cgaagccgcc
     2941 tcgcgccttc ggatcgagat cgacagcctc cccgccgaga tcgacaccct ggagcggaag
     3001 cgccgccagc tggagatcga gcgtctcgcc ctgaagcgcg agaaggacga gggctccaag
     3061 gcgcggcgtc gcgcgatcga agaggagatg gaggagaccg gagccgagtt ggcccgcctg
     3121 cgcgcgcact gggagaagga gcgcgcgcgc atccagaaga tcaacgagat caaggggcgg
     3181 atcgaggagc tccg
//
LOCUS       DASSLZ010000239         5960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_713342, whole genome shotgun sequence.
ACCESSION   DASSLZ010000239 DASSLZ010000000
VERSION     DASSLZ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713342"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1373)
                     /locus_tag="VFM00_08915"
     CDS             complement(6..1373)
                     /locus_tag="VFM00_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008984418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7498409.1"
                     /translation="MNRAKALLDRRLAAIFVLGMASGYPFVLWGSAMTAWLKESGLTR
                     SAIGVFGTVAAVYSLHFLWSPLVDRVRVPVLGRMGQRRGWILGMQIPLALCTLALALT
                     HPANSLRWTGILALAIAFFSATQDVAIDAYRVEIIPRDETAKIAHASAVTTAGWWSGY
                     ALLGAIPFLLADRPGWTWNRIYVLLAAMWLPLMAAVFAVRESAQRRDRFQEAERRYAE
                     ALARKASPGRWTRFTAWLGVTLLEPLREFFERTGARLGISVMLFLLLFKLGEAFLGRM
                     AIVFYKEVGYTDTQIGYFSKMGTGVVTIAFSMLGGFLNARIGLIRGLLVGGLAMASSN
                     LMFSWIALDAARVGHPSQSLFAAAIVVDGFTSAMSTVAFVAFISYLTSHTYTATQYAL
                     LAALGTLGRTVVSGASGFVVDALGGNWSLFFVITAVMVLPSLILLLFVGKLLRERARQ
                     WSAEA"
     gene            1478..1681
                     /locus_tag="VFM00_08920"
     CDS             1478..1681
                     /locus_tag="VFM00_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498410.1"
                     /translation="MRRIGLLLALLVVLDGGLVVLLAILKTVSLYAGAALVLLVSLAF
                     YAAVLGIMRLRRRRRVERSIEIS"
     gene            complement(1711..1793)
                     /locus_tag="VFM00_08925"
     tRNA            complement(1711..1793)
                     /locus_tag="VFM00_08925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1758..1760),aa:Leu,seq:taa)
     gene            complement(1828..2229)
                     /locus_tag="VFM00_08930"
     CDS             complement(1828..2229)
                     /locus_tag="VFM00_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7498411.1"
                     /translation="MRDAMLPLAKAVMLEYRMKPQQILYALQDSVHTRFPVYDGEPTR
                     VVGIVNAKDLFAIYATSGLVNLEDAMYPVTWAPADRSTADQLKEFRRARRQMAVVIDE
                     AGIPIGIITLEDIVEELVGDIEDEHDVSGVR"
     gene            complement(2272..2904)
                     /locus_tag="VFM00_08935"
     CDS             complement(2272..2904)
                     /locus_tag="VFM00_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HET7498412.1"
                     /translation="MSGFWGHFLTWVQVFAIPLLVLGNAVFVAAEFSLVTVRRTRLEE
                     LAANKVPGTHVAQEAVRRVTDMIAATQVGITMTSLGLGWVGEPGLARVLKPWFHFMPE
                     NWGIFAAHGVATAFAFLIITFLHVVVGELAPKGIALRKPDTVAIILAGPLLAFERIFR
                     PFIRVVNWSGNGLLRLLGFKPAAHTGHVHSVDELQLIVEDVSEAGKMSPE"
     gene            complement(2922..4805)
                     /locus_tag="VFM00_08940"
     CDS             complement(2922..4805)
                     /locus_tag="VFM00_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HET7498413.1"
                     /translation="MSANPRAPELPADFAWLNTDRPLTFARELRGQVVLLDFWTYCCI
                     NCMHVLPDLAYLEDKYAREPFLVIGIHSAKFENEGHRPTVRAAINRYEIRHPVIVDQE
                     MRLWSEYTVKSWPTLVLVGADGRIVGAVAGEGNRESLDQAIGQALADARAKGILADAP
                     LQLEREQSVRAATGLAFPGKVHADLLGQRLFIADSNHNRIVVTTLPDDEGRSRLLCTV
                     GSGRVGREDGAAETAAFHHPQGLALMNGDRLLVADTENHLLRSVDLKTWRVSTIAGTG
                     EQSYDRSGGKRGTAQGLNSPWDLAVEGSTCYVAMAGHHQIWRVDLPMALCRAWAGTGR
                     ENIVDGPAEAAALAQPSGLALMGNYLYFADSEVSAVRRVDMAAEKVETLVGRGLFHFG
                     DQDGAVEQAKLQHPLGVAACNKKILVADTYNHKIKELDPDARTIRTLLGDGKPGSERG
                     GKDLALFEPGGLHATDDTLYVADTNNHRVVRVALPSLAWAEVVVEGLEPPSVEPRSRA
                     LGAALSAVPATLRAGAATGWRVLVQLPEGAHVSDEAPASVRVQRDGQVLLQKTMLGAT
                     WPLAFELPAQPPGRVDLHVQVSFAYCHEGQGICIPASPAWSVPVRFEDRGEAEAQLTA
                     SLA"
     gene            4861..5601
                     /gene="ribA"
                     /locus_tag="VFM00_08945"
     CDS             4861..5601
                     /gene="ribA"
                     /locus_tag="VFM00_08945"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET7498414.1"
                     /translation="MRLTQAEIEAILRSEADHCCGGIGEQKICVKIVAIAELPTRFGR
                     FQIAAFWNNRDGKEHVAVMSGRPYGQESVPVRIHSECLTGDALGSLRCDCRDQLEVSL
                     RTIAQLPMGVVLYLRQEGRGIGLLNKIRAYGLQDRGLDTVEANEALGFRDDEREYAIA
                     AHMLQSLKIRSVQLMTNNPRKVSELARYGIVVEGRQPLVIPPNEHNRFYLETKAFKSG
                     HYIDFSGKPHLPEQGETVRVEGMNEGVN"
     gene            5660..>5960
                     /locus_tag="VFM00_08950"
     CDS             5660..>5960
                     /locus_tag="VFM00_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498415.1"
                     /translation="MVPHAGSGRIAKFLLIVVAALALAMALATLLGGPRRVAAQDADG
                     TEEVRIGLPSYVVTDPPGRERIMYADSSVSVNVICPVRKARVDPNRNPVYVNGKAI"
BASE COUNT          975 a         2066 c         1995 g          924 t
ORIGIN      
        1 tcgcttcagg cttcggcact ccactgacgc gcccgctccc ggagcagctt ccccacgaac
       61 agcagcagga tcaggctggg caggaccatc accgcggtga tcacgaagaa gagcgaccag
      121 ttccctccga gcgcgtccac gacgaacccg ctggccccgg agaccaccgt gcgccccagg
      181 gttccgaggg cggccagcaa ggcatactgc gtagcggtgt aggtgtggct ggtcaggtag
      241 gagatgaagg ccacgaaggc caccgtggac atcgcggacg tgaagccgtc caccacgatc
      301 gccgcggcga acaggctctg gctgggatgc cccacccggg cggcgtccag ggcgatccac
      361 gagaacatca gattggacga cgccatcgcc agcccgccga cgagaagtcc cctgatgagc
      421 ccgatccgcg cgttgaggaa tccgcccaac atcgagaagg cgatcgtcac cacgcccgtc
      481 cccatcttgg agaagtagcc gatctgcgtg tcggtgtagc cgacctcttt gtagaagacg
      541 atggccatcc tgcccaggaa cgcctccccg agcttgaaga ggaggaggaa cagcatcacg
      601 ctgatcccca gccgcgcgcc cgtgcgctcg aagaactcgc ggagcggctc cagcagggtc
      661 acgccgagcc acgcggtgaa ccgggtccat cgcccgggcg acgccttgcg cgccagcgcc
      721 tccgcgtacc gccgctcggc ttcctggaaa cgatcgcggc gctgcgcgct ctcccgcacc
      781 gcgaacaccg cggccatcag cggcagccac atcgcggcga gcagcacgta gatccggttc
      841 caggtccaac ccggccggtc ggcgagcagg aagggaatcg ccccgagcag cgcatagccg
      901 ctccaccacc cggccgtcgt gacggccgag gcgtgggcga tcttcgccgt ctcgtcgcgc
      961 gggatgatct ccacacgata ggcatcgatc gccacgtcct gcgtcgcgga gaagaaggcg
     1021 atggcgagtg ccagaattcc ggtccatcgg aggctgttcg ccggatgggt cagcgccagg
     1081 gccagcgtgc atagggcgag cgggatctgc atccccagga tccagccgcg gcgctgtccc
     1141 atgcgcccga gcaccgggac tcgtacgcga tccaccagcg gcgaccagag gaagtgcagc
     1201 gagtagacgg cggccaccgt gccgaacacg ccgatcgccg agcgcgtgag ccccgactcc
     1261 ttgagccacg ccgtcatggc ggagccccag aggacgaagg ggtagccgct cgccatgccg
     1321 aggacaaaga tggcggcgag gcggcggtcg aggagcgcct tcgcgcgatt caacgtgcta
     1381 ccatcggcaa gtaccgctca tccattatgg aaccggccct gggcccgttc cgtagccggc
     1441 ggttgtcccc gatccgagcc cattggaagg tgctgccatg aggcgcatcg gacttttgct
     1501 cgcgctcctc gtcgtgctcg acggcggcct cgtcgtgctc ctggcgatcc tgaaaacggt
     1561 ttccttgtat gccggcgcgg cgctggtact cctcgtttcg ctcgcattct acgcggccgt
     1621 attgggcatc atgcggctca ggcgacggcg gagggtggag cgcagcatcg agatctcgtg
     1681 aaggcgcggc tgaggccgca cgtgtcgttc tgcccgggga aggaatcgaa ccttcacggg
     1741 gtttccccca ggggatttta agtcccctgc gtctgccgtt tcgccacccg ggccctaact
     1801 ccaacgaccg tgccgccgac gcggcgccta ccgcaccccc gacacgtcgt gctcgtcctc
     1861 gatgtccccg accagctctt cgacgatgtc ctccagcgtg atgatcccga tcgggattcc
     1921 cgcttcgtcg atgaccaccg ccatctgccg ccgcgcgcgg cggaattcct tgagctggtc
     1981 ggccgtgctc cggtcggcgg gcgcccacgt gacgggatac atggcgtcct cgagattcac
     2041 gagaccgctc gtggcgtaga tcgcgaacag atccttcgcg ttcacgatcc ccacgacgcg
     2101 cgtcggctcg ccgtcgtaca ccgggaagcg cgtgtgaacg gaatcctgta acgcgtacag
     2161 gatctgctgc ggcttcatcc tgtactcgag catgaccgcc ttggccagcg ggagcatggc
     2221 gtcgcgcacc ttcttctcgg gaagctggaa ggcgcgcgtc aggatctcgg cctactcggg
     2281 cgacatcttt cccgcttcgc tcacgtcctc gacgatcagc tgcagctcgt ccaccgaatg
     2341 gacgtggccg gtgtgcgccg cgggcttgaa ccccaacagc cggagcaggc cgttgccgct
     2401 ccagttcacc acccggatga agggacggaa gatccgctcg aacgcgagca gcgggccggc
     2461 caggatgatc gcgaccgtgt cgggcttccg cagcgcgatc cccttcggcg ccagctcccc
     2521 cacgacgacg tggaggaagg tgatgatcag gaacgcgaac gcggtggcga cgccgtgcgc
     2581 cgcgaagatt ccccagttct cgggcatgaa gtggaaccag ggcttgagca cgcgcgccag
     2641 ccccggctcg cccacccacc ccaggcccag gctggtcatc gtgatcccga cctgggtcgc
     2701 cgcgatcatg tccgtgaccc ggcggaccgc ttcctgcgcc acgtgggtgc cgggcacctt
     2761 gttcgcggcc agctcctcca gccgcgtcct gcgcacggtg accagcgaga actcggcggc
     2821 cacgaagacg gcgttgccca gcaccaggag cgggatggcg aagacttgga cccaggtgag
     2881 gaagtgtccc cagaagccac tcatgcgagc gcccgggggc cctaggctaa cgacgcggtg
     2941 agctgggctt ccgcttcacc ccggtcctcg aagcggacgg gaacgctcca ggcggggctg
     3001 gccgggatgc agatgccctg cccttcgtgg cagtaggcga aggagacctg gacgtggagg
     3061 tcgacgcggc cgggcggctg cgccggaagc tcgaaggcga gcggccacgt ggcgccgagc
     3121 atcgtcttct ggagcaggac ctggccgtcg cgctgcaccc gcacgctggc cggcgcctcg
     3181 tcgctcacgt gggccccctc ggggagctgc accaggacgc gccatccggt cgcggcgccg
     3241 gcgcgcagcg tcgcgggaac cgccgagagc gccgcgccca gggcgcggga tcgcggctcc
     3301 accgagggcg gctccagccc ctccacgacc acctccgccc aggccaggga cgggagcgcg
     3361 acccgcacca cgcggtggtt gttcgtgtcc gccacgtaca gcgtgtcgtc ggtcgcatga
     3421 aggccgcccg gctcgaagag cgcgaggtcc tttccgccgc gttcgctccc gggcttgccg
     3481 tccccgagaa gggtacggat agtccgcgcg tcggggtcca gttccttgat cttgtggttg
     3541 taggtgtcgg cgaccaggat cttcttgttg cacgcggcca cgccgagcgg gtgctgcagc
     3601 ttcgcctgct cgacggcgcc gtcctggtcg ccgaagtgga agaggccgcg gccgaccaac
     3661 gtctccacct tctcggcggc catgtccacg cggcggaccg cgctgacctc ggagtccgcg
     3721 aagtagaggt agttccccat cagcgcgagt cccgagggct gcgccagcgc ggcggcctcg
     3781 gcgggcccgt ccacgatgtt ctcgcgcccg gtgcccgccc aggcgcggca gagcgccatc
     3841 ggcaggtcca cgcgccagat ctggtggtgg cccgccatcg cgacgtagca cgtgctcccc
     3901 tcgaccgcga gatcccaggg cgaattgagc ccttgcgccg tgccgcgctt gccgccggag
     3961 cggtcgtacg actgctcccc ggtgcccgcg atggtgctca ccctccaggt cttgagatcg
     4021 accgaccgaa gcaggtggtt ctcggtgtcg gccacgagga gccggtcccc gttcatcagg
     4081 gccagtccct gcggatggtg gaacgccgcg gtctcggcgg cgccgtcctc gcggcccacc
     4141 cgtcccgatc ccacggtgca caggagacgt gaccgtccct cgtcgtccgg gagggtcgtg
     4201 actacgatcc ggttgtggtt cgaatcggcg atgaagagac gctgtccgag cagatcggcg
     4261 tggacctttc ccgggaaggc gagacccgtc gcggcgcgca ccgactgctc gcgctccagc
     4321 tggagcggcg catccgccag gatccccttc gcgcgggcgt cggccagtgc ttgaccgatc
     4381 gcctggtcca gggactcccg gttgccctcc ccggcgaccg cgccaacgat ccgcccgtcc
     4441 gcgccgacca ggaccagggt gggccaggac ttgacggtgt actcgctcca gagccgcatc
     4501 tcctggtcca cgatcacggg atggcggatc tcgtagcggt tgatcgccgc ccggactgtg
     4561 gggcggtgcc cctcgttctc gaacttggcg ctgtggatgc cgatcaccag gaagggctcc
     4621 cgggcgtact tgtcctcgag ataggcgaga tcggggagga cgtgcatgca gttgatgcag
     4681 cagtacgtcc agaaatcgag caggaccacc tgaccgcgca gctcccgcgc gaacgtcagg
     4741 gggcggtcgg tgttgagcca ggcgaagtcc gccggcagct cgggggcccg gggattggcg
     4801 ctcacggggc tacgatagca cgtttcgatt cgaaaccggg ccggaggcat ctaatccacc
     4861 gtgcgactga cccaggctga aatcgaggcc atcctccgct cggaagcgga ccactgctgc
     4921 ggggggatcg gcgagcagaa gatctgcgtc aagatcgtgg ccatcgcgga gcttcccacc
     4981 cggttcgggc ggttccagat cgccgcgttc tggaacaatc gcgacggcaa ggagcacgtg
     5041 gcggtcatgt ccggccgccc ctacggccag gagagcgtcc ccgtccggat ccattcggaa
     5101 tgcctgaccg gagatgccct ggggtcgctc cgctgcgact gccgcgacca gctcgaggtt
     5161 tcccttcgga cgatcgccca gctcccgatg ggggtcgtcc tctacctgcg ccaggaaggg
     5221 cgcgggatcg gcctcctgaa caagatccgg gcgtacggcc ttcaggaccg cggcctggac
     5281 acggtcgagg ccaacgaggc gctcggcttc cgggacgacg agcgggagta cgccatcgcc
     5341 gcccacatgc tccagagcct gaaaatccgg tcggtccagc tcatgaccaa caatccgcgc
     5401 aaggtgagcg aactggcccg gtacgggatc gtcgtcgagg ggcgccagcc cctcgtcatc
     5461 cccccgaacg agcacaaccg gttctacctc gagaccaagg ccttcaagtc gggtcattac
     5521 atcgactttt ccggaaagcc tcatcttccc gagcagggag aaaccgtccg ggtggagggt
     5581 atgaacgagg gagtgaattg aagtcgaagg gagtggccga cttcggttac gatctcgggt
     5641 cctggctctg ggggggcact tggttccaca cgctggctct ggacgcatcg ccaaattcct
     5701 gctgatcgtt gtcgccgcgc tcgccctggc catggccctg gcgacgctcc tgggcgggcc
     5761 gcgccgcgtg gcggcccagg acgctgacgg aaccgaagag gtccggatcg gcctgccctc
     5821 ctacgtcgtc accgatcccc cgggcaggga gcggatcatg tacgcggact cgagcgtctc
     5881 cgtcaacgtc atctgcccgg tccgcaaagc ccgcgtcgat ccgaaccgca atcccgtcta
     5941 cgtgaacgga aaggcgatcg
//
LOCUS       DASSLZ010000240         4594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_206388, whole genome shotgun sequence.
ACCESSION   DASSLZ010000240 DASSLZ010000000
VERSION     DASSLZ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206388"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFM00_08955"
     CDS             complement(<1..191)
                     /locus_tag="VFM00_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498416.1"
                     /translation="MSATFPGIGIWISALLTLFAFSFLYKDNPFFRFAESLFAGVSLG
                     YYIGIVLNQTLVPNLYDPL"
     gene            complement(226..1104)
                     /locus_tag="VFM00_08960"
     CDS             complement(226..1104)
                     /locus_tag="VFM00_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498417.1"
                     /translation="MQRFFHWMLALDRRWIYLFVLVSLITPIVVPIGLPVTTTGSTRK
                     AFEFIERLKPGDVVWISYDYGPSSAPENDPMAEAFLRQCFLKKLRVIVCAFYPLGGLG
                     LANNSVARIVAETPGLRYGVDYINLGYKDGAAAVMRRLGDNIADAFPTDVNGRKIEEF
                     PIMKGIHDIHHVSLVFTVATGIIGEYWITQVHAQFGTPIIIGPTAVSAPKYYAYLNAG
                     QLEGMLGGMKGAAEYEKLLMAKYPSLNRYYHETKYFTATKGMDGQTVLHTVILMFIFL
                     GNVAFLVTRGKERQAA"
     gene            complement(1119..1922)
                     /locus_tag="VFM00_08965"
     CDS             complement(1119..1922)
                     /locus_tag="VFM00_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498418.1"
                     /translation="MNLEFGRVALVLCFFVLPAIGIALGLGSPAMRSRIPLIVAFIAG
                     IVPILSLYVPKNFLSGETGMSPRLDQWLIIVAGFALLLGIVNVIQNSSRKVARREAGW
                     FFSAVLLTGLFVMGSFSIWGAVSGQGIGFAPDGSSTPFKWMEDRFFQPLQSTIFSLLA
                     FFMASAAFRAFRARNTEATILLFAAGLVMAGRVPLLEFLAVPFPPLQPAAATASQALG
                     RLTEWIMDTPNGAAQSGIIIGAALGAASMAVRVILGIERGYLGLGSSES"
     gene            complement(1954..3186)
                     /locus_tag="VFM00_08970"
     CDS             complement(1954..3186)
                     /locus_tag="VFM00_08970"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HET7498419.1"
                     /translation="MRWAVGSFLLAVLFTPAAVSAQGAAPPSPPASTVPTQALPAPTN
                     FVAQDNPGDSGGSILLTWTDPDSAALPAGSVIEIRRAEAPAFDWKALAEVPLGTQTYK
                     DASATEKHTYRYELRARAPAAPADTTRAAAAAASAAAAGTAVVSEPASAHDNWFRKDR
                     TNSLIATIIFLIALLGSIQMAHSGKPIFIRRIAGLNAVDEAIGRATETGRKVLYVPGI
                     QSMDDIQTVASMSILGHVARATARYGTDLDVANKDPLTFASARETVRGAYLAEGRPDL
                     YREEMVNYVTYDQFAFTAAVSARMVRERPATIFLVGYFFAESLILAETGQSVGAIQIA
                     GQADPTQLPFFVATCDYTLIGEELYAASAYLSREPVLLGSIRGQDIFKALCIVVGLAG
                     IAFATFGSTWVAKLLQTQ"
     gene            complement(3219..4277)
                     /locus_tag="VFM00_08975"
     CDS             complement(3219..4277)
                     /locus_tag="VFM00_08975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7498420.1"
                     /translation="MTPEATARGPASPAAHGPASGTVARRLDSLRAILMQSDAALREK
                     LPEYFLVYYKHLNILRNPARIEHVLQKGRAMYDYLEAEGKDVLDLGAGFGIEALLVAI
                     YGARRVLATEIDRDMVAVGAYLAQAVDPPLSPEHFESRYGDGIAMELPSASFDGVMAN
                     CVISHVRDLEGFLREANRLLRPGGIFFLSDENNSLYLPARGRRRRGWRDMEAEPNGPY
                     FAARRALIGEHFPSLARNQLFEATRATRGLVGADVIEGTRELLETGAVRRRPEFLYRD
                     PRDGQYPERELNPFWMMGKFRDAGLEPELLPAFFSRKIETHPRQWVKDVLRIFCTRWP
                     VLSVYLWPIMRIRGRKVR"
     gene            complement(4274..>4594)
                     /locus_tag="VFM00_08980"
     CDS             complement(4274..>4594)
                     /locus_tag="VFM00_08980"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7498421.1"
                     /translation="YPWPGNVRELENAMARAVALADEGAPLEPSLFGLGAPVSRRWDG
                     QHTLRETLDAVEAETIREALRQCESNVSRAARALGVSRQHLHNRMNFHGIRRARILSE
                     SNGQ"
BASE COUNT          753 a         1519 c         1617 g          705 t
ORIGIN      
        1 ttcagcggat catacaggtt cggcaccagc gtctggttca gcacgatgcc gatgtagtag
       61 ccgagcgaga cgcccgcgaa gaggctctcc gcgaagcgga agaagggatt gtccttgtag
      121 aggaacgaga acgcgaagag cgtgaggagc gccgaaatcc agatcccgat tccggggaag
      181 gtcgcgctca cgcccgggtc tccttcgatc cgccgcccat ccggctcatg ccgcctgtcg
      241 ctccttcccg cgcgtgacca ggaacgccac gttccccagg aagatgaaca tcaagatcac
      301 ggtgtggagc acggtctgcc cgtccattcc cttcgtggcc gtgaagtact tggtctcgtg
      361 gtagtagcgg ttcagcgacg ggtacttggc catgaggagc ttctcgtact ccgccgctcc
      421 cttcatgccg ccgagcattc cctcgagctg ccccgcgttc aggtaggcgt agtacttggg
      481 cgcgctcacc gcggtcggcc cgatgatgat cggcgtgccg aactgggcat ggacctgcgt
      541 gatccagtac tccccgatga tcccggtggc cacggtgaag accagagaga cgtgatgaat
      601 gtcgtggatc cccttcatga tcgggaactc ctcgatcttg cgcccgttca cgtcggtcgg
      661 gaaggcgtcg gcgatgttgt cgcccaggcg ccgcatcacc gcggccgcgc cgtccttgta
      721 gccgagattg atgtagtcga cgccgtagcg gagtcccggc gtctcggcca cgatgcgcgc
      781 gaccgagttg ttcgcgagcc ccagcccgcc gaggggatag aaggcgcata cgatgacgcg
      841 cagcttcttg aggaagcact ggcgcaggaa cgcctccgcc atcggatcgt tctcgggcgc
      901 cgacgagggg ccgtagtcgt aggagatcca gaccacgtcg ccgggcttca gccgctcgat
      961 gaactcgaac gccttgcgcg tggacccggt ggtggtgacc ggcaggccga tcggcacgac
     1021 gatcggcgtg atgaggctca ccagcacgaa gagatagatc caccgccggt cgagagccag
     1081 catccagtgg aagaagcgct gcatctagcg gctcccgctc atgattcgct gctccccaga
     1141 ccgagatagc cgcgctcgat ccccaggatg acgcggaccg ccatcgaggc cgcgccgagt
     1201 gccgcgccga tgatgatccc cgactgcgcg gcgccgttgg gcgtgtccat gatccattcg
     1261 gtgagccgcc cgagggcctg gctggccgta gccgcggcgg gctggagcgg tgggaatggg
     1321 accgccagga actcgaggag cggcacgcgc cccgccatca cgagccccgc cgcgaacagg
     1381 aggatggtcg cctccgtgtt ccgcgcgcgg aacgcgcgga acgcggccga ggccatgaag
     1441 aaggccagca gcgagaagat cgtcgactgg agcggctgga agaaccggtc ctccatccat
     1501 ttgaacggcg tggacgaccc gtccggggcg aagccgattc cctgccccga gaccgcgccc
     1561 cagatggaga aggagcccat cacgaaaagc ccggtcagga gcacggcgga gaagaaccac
     1621 cccgcctcgc ggcgcgcgac cttccggctg ctgttctgga tcacgttgac gatgccgagg
     1681 aggagcgcga agccggccac gatgatgagc cactggtcca ggcgggggct catccccgtc
     1741 tcgccggaga ggaagttctt gggaacgtag agcgagagga tcgggacgat cccggcgatg
     1801 aacgcgacga tcagcggaat ccggctccgc atcgccgggc tccccagccc cagcgcgatg
     1861 ccgatcgcgg ggagcacgaa gaagcagaga accagcgcga cgcgtccgaa ctcgaggttc
     1921 acgctgcggt ctcctccggg cgcgtggtcg ttctcattgg gtctggagaa gcttcgcgac
     1981 ccacgtgctg ccgaacgtgg cgaatgcgat tccggccagg ccgaccacga tacagagcgc
     2041 cttgaagatg tcctgcccgc ggatcgagcc caggagcacc ggctcgcgcg agagataggc
     2101 gctggcggcg tacagctcct cgccgatcag cgtgtagtcg caggtcgcga cgaagaaggg
     2161 gagctgggtg ggatcggcct gccccgcgat ctggatcgcg ccgaccgact gccccgtctc
     2221 cgccaggatc agcgattccg cgaagaagta cccgaccagg aagatcgtgg ccggcctctc
     2281 gcgcaccatg cgcgccgaga ccgcggcggt gaaggcgaac tggtcgtacg tcacgtagtt
     2341 caccatctcc tcgcggtaga ggtcgggacg cccctcggcg agataggcgc cgcgcaccgt
     2401 ctcgcgcgcg gaggcgaacg tgagcggatc cttgttcgcg acgtcgaggt cggtgccgta
     2461 gcgcgcggtg gcgcgcgcga cgtggcccag gatcgacatc gaggccaccg tctggatgtc
     2521 gtccatgctc tggatgcccg ggacgtagag caccttcctc cccgtctcgg tcgcgcggcc
     2581 gatcgcctca tccacggcgt tcaggccggc gatgcgccgg atgaagatcg gcttgccgga
     2641 gtgggccatc tggatcgagc cgaggagggc gatgaggaag atgatggtgg cgatgaggga
     2701 gttggtgcgg tccttccgga accagttgtc gtgcgccgac gctggctcgc tcacgacggc
     2761 ggtccccgcc gcggcggccg aggcggcggc ggcggccgcg cgggtcgtat cggccggggc
     2821 ggccggggcg cgtgcgcgaa gctcgtaccg atacgtatgc ttctccgtcg cgctcgcgtc
     2881 cttgtaggtc tgcgttccga gcggcacctc ggcgagcgcc ttccaatcga acgccggcgc
     2941 ctcggcgcgc cggatctcga tcactgatcc cgcgggaagc gcggcgctgt cgggatcggt
     3001 ccacgtgagg aggatcgagc cgccggaatc gccgggattg tcctgggcga cgaagttcgt
     3061 gggagcgggg agtgcctggg tcggtacggt cgacgcgggg ggcgacggcg gcgcggcgcc
     3121 ttgggccgat accgccgcgg gcgtgaacag gacggccagg aggaacgacc ctacggccca
     3181 tcgcacgggc gcagcctatc ggcgcgccgc gcggggtgtc aacgtacttt tcgcccccgg
     3241 atccgcatga ttggccacag gtagacggac agcaccggcc agcgggtgca gaaaatgcga
     3301 agaacgtcct tcacccactg cctgggatgc gtctcgatct tccgggagaa gaaggcggga
     3361 aggagttcgg gttccagccc ggcgtccctg aacttcccca tcatccagaa gggattcaac
     3421 tcgcgctcgg ggtactgccc gtcgcgcggg tctcgataga ggaattccgg gcggcgccgg
     3481 acggcgcccg tttcgagaag ctcgcgcgtc ccctcgatca catcggcgcc cacgaggccc
     3541 cgcgtcgcgc gggtcgcctc gaagagctgg tttcgcgcga gcgacgggaa gtgctcgccg
     3601 atcagggcgc ggcgcgccgc gaagtagggg ccgttcggct ccgcttccat gtcgcgccac
     3661 ccgcgccggc ggcgtccccg cgccggcagg tagagggagt tgttctcgtc gctcaagaag
     3721 aagatgccgc ccggccggag cagccggttg gcctcacgca ggaatccctc gaggtcgcgg
     3781 acgtgggaga tcacgcagtt ggccatcacc ccgtcgaatg acgccgacgg gagctccatc
     3841 gcgatcccgt ctccatatcg ggactcgaag tgctcgggcg aaagcggcgg atccaccgct
     3901 tgcgccaggt aggcgcccac cgcgaccatg tcgcggtcga tctccgtggc cagcacccgc
     3961 cgggcgccgt agatcgcgac gaggagcgcc tcgatcccga accccgcccc caggtcgagc
     4021 acgtcctttc cctcggcttc caggtagtcg tacatcgcgc gccccttctg gaggacgtgc
     4081 tcgatccgcg ccggattccg gaggatgttc agatgcttgt agtagacgag gaagtactcg
     4141 gggagcttct cgcgcagcgc ggcgtcgctc tgcatgagga tcgcgcggag cgagtcgaga
     4201 cggcgggcga cggttccgct cgcgggcccg tgcgccgccg gcgaggcggg cccgcgcgcg
     4261 gtcgcttcag gggtcactgc ccgttggatt cgctgaggat ccgcgcgcgg cggatcccgt
     4321 ggaagttcat ccgattgtgg aggtgctgcc ggctcacgcc cagggcgcgc gccgcgcgcg
     4381 agacgttcga ctcgcactgg cggagcgcct cgcggatcgt ctcggcctcg acggcgtcca
     4441 gcgtctcccg cagcgtgtgc tggccgtccc agcgccggga caccggcgcc cccagtccga
     4501 acagagaggg ctcgagcggc gccccttcgt cggccagcgc gaccgcgcgg gccatcgcgt
     4561 tctccagctc gcggacgttc ccgggccacg ggta
//
LOCUS       DASSLZ010000241        20674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1346455, whole genome shotgun sequence.
ACCESSION   DASSLZ010000241 DASSLZ010000000
VERSION     DASSLZ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 20674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20674
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346455"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VFM00_08985"
     CDS             <1..611
                     /locus_tag="VFM00_08985"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7498422.1"
                     /translation="MQVRTPVRYLANGVDIVNGVFVQNGSTLRGEVISPIPLTSDSLS
                     LRIDGIPVAATITKLDAPGRQWALEYVASDLFTGTHTIQLFVGAQGFEQRTFQTTSEF
                     TLRGVAVVDPRMQGTGCGGSIFQYELSAAARKVELQLFTVAGRRVASIDLPGQAGFNL
                     FCWDGRDSRTHDTAQGVYLYRLRATDPNGKTVTRDGRMIRAR"
     gene            659..1594
                     /gene="murQ"
                     /locus_tag="VFM00_08990"
     CDS             659..1594
                     /gene="murQ"
                     /locus_tag="VFM00_08990"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HET7498423.1"
                     /translation="MARGADRRSGKAIFRELSKLVTEQRNPRSRRLDRLSTAQTLRLM
                     NAEDRRVPLAVGREIPKIARAVDLMVERLERGGRIFYVGAGTSGRLGVLDAAECPPTF
                     GTPRSLVQGIIAGGRRALVRSIEGAEDDAGAAVAALKKKRVGPKDVVVGIMASRRTPY
                     AIGAVEHGRKVGAATIVVTANPSSDARFDVDVVIAPRVGPEVVTGSTRMKAGTAQKLV
                     LNMLSTATMVRMGKVYENLMVDLKTASRKLEERTKRVFMYATGARYEEAEPALKQAGG
                     SLKVAIVMRRAKVGRSEAERRLKRAQGWVRQAIEN"
     gene            1645..2871
                     /locus_tag="VFM00_08995"
     CDS             1645..2871
                     /locus_tag="VFM00_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7498424.1"
                     /translation="MAATLIASMLVVACGAGCAKSRSGKTTVVFWQFSPLATIQPILD
                     RYRAENPGVDLQVEQLTWQSGREKIVAAIAAGRPPDLCELGSTFLPGLVADSTLVDLS
                     DSIPDLRAELVGWDAVSYRGRAFALPWMLGTRALFINEDLFRKAGLDPAKPPATWAEL
                     LDDARRVTQRVPDAKGFGMNSGEREILIKKFMPFAWGNGGDILDSTLTRSVFNSPQNV
                     EALKFYLSLKPYSLLDRQEMHEQAFAKGRLGIVISGPWLLRTLAKTAPDAHVQVGLMP
                     RPAANRGKSASFAGAEVLGIFRGSKHRAEALRLARFLVQPRNSMPLYVATGNAFPAAT
                     SAAADSYFTTHPMDRLFLEQLHTAVSPPLHPRWVEIEEIVNAELEQAIYGTKTPAAAL
                     AEGDAKIAQVLAGPKP"
     gene            2909..3790
                     /locus_tag="VFM00_09000"
     CDS             2909..3790
                     /locus_tag="VFM00_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7498425.1"
                     /translation="MRGSRPSIGLLPWGLAFLVFGLFPLLYALVLSFQQMNPLRPDLT
                     HFVGVSNYARALTSASFWHSLRTTVFFVVGTIPVTLTLAYLVAGQLATIRRGQGFFRA
                     AIFFPATVSMVVIALVFKSLYAEDGLLNGWLAASGVSRVHWLQDVRTALPSIMLMDIW
                     GSVGYYAILILAGRKTLPPEQLEAARLEGLSAWEVERRIVFPHLRPVLLFVVLLNTIR
                     SFQIFTEVFVLTRGGPLESTLTLVYHLYERAFYHFEMGYASAIAYLLLALVGVVLLVQ
                     RRVLMSRREAPLSGGVG"
     gene            3787..4602
                     /locus_tag="VFM00_09005"
     CDS             3787..4602
                     /locus_tag="VFM00_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7498426.1"
                     /translation="MKPRPLGAYVILSAIALLSFAPLLYMARVSLGQGGDLPISPSDW
                     WGRPFTLDHYRALAAGGPMARFAINSIVVSCVALPLQLLLAASAGHALARRRFLGKNV
                     TLALATAFLVLPRQVTLVPLYLLMAKLGLMDTYAALILPHLADPFGVLFMAHYYQTMS
                     AELEDAARTDGLSAGAIFRRIVLPLSGPGLAVVGVNGFLTTWNAFYQPLVLTTSESMR
                     TLPVGLALFAQSEYSVDWGALLAGSSIATLPVLVAFALFQRQIVEGVLQGSGR"
     gene            4599..5822
                     /locus_tag="VFM00_09010"
     CDS             4599..5822
                     /locus_tag="VFM00_09010"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HET7498427.1"
                     /translation="MTADKRGAWRPEPALIRKTTATAGTVRAIGLMSGTSADGMDAAA
                     VLIEGGDAPRRATKLVALRSVPFERELRAEILFAQEGELPLRRLFALAVALGEVGADA
                     ARQAHAAAAWNEPPDVIGFHGQTVFHDPRGERSGRPLTTQIGEPAVIAHALGAPVVSG
                     FRMADVLEGGEGAPLVPRFDYNQFGSDTADRVLLNLGGIANLTRLPNGAPIGAITAFD
                     CGPANMLLDGIVAALRPEGPAYDAGGALAAGGRVDEAVVREFLGHPFFARKPPKSAGR
                     EEFGAEFRARFLSRTEGRSLEDRLRTAVEITAAGVARGVEQSGPRAPDEIIVSGGGAK
                     NVVLLDAIRARLPKSVVRTSDELGVPSEAKEAMAFAFLAAETMAGRPGNVPAATGARK
                     EVVLGSVTMPPDPKR"
     gene            6103..7302
                     /locus_tag="VFM00_09015"
     CDS             6103..7302
                     /locus_tag="VFM00_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HET7498428.1"
                     /translation="MSDEWRTRDVVRIGLKVGAPDLLLAGSSAWKLETPDGNDLAHAG
                     PGEVLRIRQRGGSIDVTKQGGGDALASTDPDDTLLLVPERGYAGWSGRWYRGTFRVYA
                     TPPGSDGTSSGLTLVNEVPLEDYLRGVLPEEIGNPPATDFEAVKAQAVAARTYTLSYL
                     SRRADLGFDLWATVEDQVYGGTARENAQASRAVQETRGEILLSDGVPIRALYSSACGG
                     RTSNVEDVWPWPWTPYLRSVRDAEDEKLTGYCSLSANYRWREEWPVDQFVATLRKYAP
                     AEGASVAALDGQILDVRIEKRSRSGRVAELVVETTEGECVLHGDRTRWALRRPGTEAI
                     LRSSFFKIGIQRAADGAPLKVIASGGGNGHGIGMCQWGAMGMARAGKNYREILRHYYK
                     DTQLAHL"
     gene            complement(7780..8790)
                     /locus_tag="VFM00_09020"
     CDS             complement(7780..8790)
                     /locus_tag="VFM00_09020"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7498429.1"
                     /translation="MSGFKEIALRESCFRPGSTLCPGCMESIALQNVGRVSDNGMKTV
                     FTLGTSCAEVSSLAFPNVVAWGRGDTPPDDFHKSFGIIHNVFESAPTLAEAARDVADL
                     LADHGAFAHPVQVIAASGDGGAFAIGLRSFLHTIYRRSRVTLMVLVNEIFANTGFQYS
                     PATTPFAETSTTPVGEASLGNPRPPLDYIHLAIAAGAGLVAQVSPAHPKLFVKTMEKA
                     LACKETAVIFVPAPCISGWKFEDGETVRLATLGAQCGVFPVFIWEKGAGGSVKDCPQE
                     ITERPAVEEFLGGQRRFHHLVQKGPDGKFAPRPGKEEAVDRLRTWTQSNVDRLYSLAT
                     LH"
     gene            complement(8787..10166)
                     /locus_tag="VFM00_09025"
     CDS             complement(8787..10166)
                     /locus_tag="VFM00_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498430.1"
                     /translation="MSKVLEKNGKAPEKSVSKASEKTAALTHCATPSGEIYPDAIDLA
                     KVRAAHHALYTKRETITADGNTAAAIAALQMWRCILFAGFPITPSTKWIETVAARVAA
                     DVGGKKRVKLLEAEHAVADYLVGAAAACRDLIFPTATSSVGLDHMTETTRSLGASGLG
                     NVMLVNVYRATANFPLCIEGDPSDTLAHRDDGFIQLACRGKQQIYDTVLQAPCLGMQP
                     DIMTPTMPGYYGIKDSHRNARLTVEPDAEIHSFQDHWIRSCPLPGLLNGDSAFGNCVT
                     SRHFQGFKVAQRRRMDRVIDILPIIGADFERKFGRPGLAYYDAVGFQNGTPPDIAVVS
                     MGPDFGTFEAIREEYQRLAWTRVAGIAMRLLTPFPREAVRARLAGAKVVLVVNQAHHQ
                     GRGHLTLDVADALSGIEKPPRIVSVFTGLGGADVSEPTWERILDHARSALDGKALPPV
                     AFFHEGTQL"
     gene            complement(10163..11131)
                     /locus_tag="VFM00_09030"
     CDS             complement(10163..11131)
                     /locus_tag="VFM00_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET7498431.1"
                     /translation="MPPIDLRERIRTSVLDVRGDGKAGGGLVLAFQSLANLMLRDSGL
                     HVQEWPFFSSARRGANIRSFLRVSAKPILAACEVTRPALSVLMDEGAARSADFTQGVP
                     HGGTFVLNTPHTPEECALHYGLTGRVVTIAGDAIGSEHLKHAIGNVSIYVAVAHAIGG
                     FEEKAIVESFLETLKKRHVPDAVVERNRGALHASLEAVREGVFEEESTRRSSAPWLGY
                     GYLPVGAQTALRLSAANQTANYAPSGLRLRFDDPKTLCNGCAHCITNCPEGIIRFVPD
                     AERGLLVTGVDVSTFCKLCGECIAVCPEHLFLEVPYEEPWMEVSRS"
     gene            complement(11140..11349)
                     /locus_tag="VFM00_09035"
     CDS             complement(11140..11349)
                     /locus_tag="VFM00_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET7498432.1"
                     /translation="MANVPEFYIDEDLCTECGDCIKAMPLAFRKVEDEEVAEVYNTAV
                     DESQKPKLDKIIAECPGHAILWRKK"
     gene            11569..12615
                     /locus_tag="VFM00_09040"
     CDS             11569..12615
                     /locus_tag="VFM00_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET7498433.1"
                     /translation="MADPTKPTKRSGTEDPQFQAGRRRFLGLAALLSGGAALAVVRPN
                     SIPGLGSLTGTPARVRRLESSRPALGTWMRVVVNDHDPKRAERAIEGAFRAVRLVDGQ
                     MSIHRPESQVSRVNAAAGRGSVRVDRALLDVVDRATAMARRTNGLYDPTVLPLMKLYG
                     FYRSGRTAWPSDREIAATLDRTGWRHVRLNRAAGTLGLARDGVALDLGSIGKGWALDR
                     AVDAIRAEGVTSALVDLGGNIYALGAPDDDAAGWSVGIPHPESGKIERVLTLRDRAVA
                     TSNDREQHHRLGRFEIGHHLDARRGRPSGGHTSATVVAKTGLESDVHSSVAYLLGPSG
                     FHGFSEIQESFFLG"
     gene            12619..13962
                     /gene="rsxC"
                     /locus_tag="VFM00_09045"
     CDS             12619..13962
                     /gene="rsxC"
                     /locus_tag="VFM00_09045"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HET7498434.1"
                     /translation="MPELKTFGRGGIHPDPHKRDTAERGIEPIDPPPNEVWLPLLQHL
                     GDPSVPIVKKNDRVVRGQKIAEAGPSGVPLHATISGKVKPIDKRPHPTLVSSEAIVIV
                     RESDEELEFPEDPGWRELGPGAALERIRDAGIVGLGGAAFPTYRKLTLPEGVNVDTFV
                     LNGAECEPYLTSDYRLMLSEAEAIVEGALAMARVVGVHRVLAGVEADKMPAVLALREA
                     AKRVPHEGCSFDVVPCEVRYPQGAERQLVAALTGRAIPPRALPLAVGVVVQNVATAHA
                     VYQALRYRKPLLDRIVTVTGSGIVYPRNVRVPLGTLLERVVDFCGGLRVDTTRLIAGG
                     PMMGRALPRLDLPITKGMNGLLALTGRGPFEMGYGPCIQCGRCVDACPLGLEPDQVSV
                     RVEAGKVLDTEPFGARECYECGCCTYVCPSGRPLLQFMQVAKSSLRRATSVRVTA"
     gene            13959..14954
                     /locus_tag="VFM00_09050"
     CDS             13959..14954
                     /locus_tag="VFM00_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HET7498435.1"
                     /translation="MSEPAIATAQGPFVLAPGPHVRTSESTASIMWTVNAALVPAAAW
                     GALMFGFPALAVIGASILGAVAAEWGAGRVMRARTSVRDGSAVCTGLLLAMTLPPRLP
                     APAAFLGAVFAILFGKMIFGGLGYNLFNPALIGRAFLMATFPLPMTAGWSPPAKWISH
                     VDAVTTATPLAALRERGIDAALKLAGGPESPLAGLFLGIRPGSIGEVSVLLLLVGGAV
                     LLLRRIISLTIPLAVVAGVALVTAPTGHVDLHLLSGGLWLGALFMATDYVTSPTTRNG
                     QIVFGLVIGGLTGVIRIYGGYPEGICYAILLANALVPALNLWFKPARPVFAGAPS"
     gene            14984..15862
                     /locus_tag="VFM00_09055"
     CDS             14984..15862
                     /locus_tag="VFM00_09055"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HET7498436.1"
                     /translation="MAPGGGSGGPGKAPSPAGEVVRIASSMAAVCAVGALILGGVYLA
                     TERYAEAARKTSEREAVTQMLGLDARATVLEVSQYLSPNGDEVVYRTASEGAVPGREL
                     VFSLDGALLKNGSAPLAPAPRELRPLGRVFAARRNGAPAGFLVEGEARGYKNRIRFFV
                     ALDTAWTVQGVRVVEHEEDPGLGAEVATPWFQGQYAGRTLADVTGLDVTKDPMPEDWR
                     GALRDLGRLTPTAWQEAHGAQRERERKHPIYAVTGATISSRAITLGVRATILHFRKRW
                     ERIAPYLETDVREASR"
     gene            15859..16533
                     /gene="rsxE"
                     /locus_tag="VFM00_09060"
     CDS             15859..16533
                     /gene="rsxE"
                     /locus_tag="VFM00_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HET7498437.1"
                     /translation="MSEQPLLFTQRPPGAVSLLKNGIFGENPVFRLALSLCPAVAVTT
                     TVANGIMLGIAVLLVQVLSSVTVALTRRWIHPRVRIPVYTIIIAVWVSAIDMVLAGFF
                     PLLYAQVGLYVKLIVAFAIIISRLEVFASRMPLIPTFWDGLGMGLGFLFALMAIGTFR
                     EVLGNGTLLGFRVVAEKPLLFFALPAGGFFSIALLMAFFNVLERRASGGKTAAPSTGG
                     SHGQAA"
     gene            16517..17476
                     /locus_tag="VFM00_09065"
     CDS             16517..17476
                     /locus_tag="VFM00_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HET7498438.1"
                     /translation="MAKPRSLVLAGTGALALCFAIGLALAAAAGRPAAPHAPSGVITR
                     ARVLAPDHVRLTVAAPVADLRPSNFRVTDERAPDVPMDVTAVETLPDGAILLTVADRL
                     DPAAGYRISIQSPAGTREAKPAPWALLFTVVISSALINNFVFTRYLGLCIFFGVSKKR
                     DTSVGMGFTFAAVMLLSAAISWGLYTFVMRPLELKFLQVIVFIGVVAFIVQALDTILR
                     KTHASLHQRFGVYLVLITTNCIILAVPLLNAQAESGVGESLALALGSGAGFALALFLM
                     SCARERLELARVPVAFQGLPIAFALAGLFALAFMGFSGLTFLR"
     gene            17481..18347
                     /locus_tag="VFM00_09070"
     CDS             17481..18347
                     /locus_tag="VFM00_09070"
                     /inference="COORDINATES: protein motif:HMM:NF005503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HET7498439.1"
                     /translation="MTDLLHVALWGFAVFTAIGVVFGLTLAGVALRFQVPVNPTVEKV
                     RAFLPSANCGACGFAGCQAYAEAVVLRNEVSPNLCVPGRAAVATALARLTGKELGSVE
                     DRIVVMRCQGTSAYARKEAEYAGLNTCAAAALVFGGPRACKNGCLGLGDCVKACPFDA
                     LSIGIDGIAVVNADRCTGCGLCVPVCPKELFQFYPRTRRIELSCVAKEKQSVVRATCM
                     VGCTLCRKCVAKCPAQAIEWDGRTIIIHHEPCLAYGPSCGEACVDICPSTILHRVGQL
                     AQPEPAEPAMEA"
     gene            18344..20128
                     /locus_tag="VFM00_09075"
     CDS             18344..20128
                     /locus_tag="VFM00_09075"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET7498440.1"
                     /translation="MTEMRAPTTPDTHPSTRSEARSGAPPRPRSLVLALVGSGGDGVA
                     LLGDLLLGMAAREGLYGMMVQSYGPQIRGGESAAILRLADHEVQYEGDQVDILLCFRL
                     RDLARFQSSLRVHPGSIVVIDQGETAEVPAWLGATADKPYRFPFARYEEGVEVDGPPK
                     NMLGLGLLCRMLGWPDTLAQDILRQRFSARRERLQSNLESLASGYAVVDPPSRPAPEG
                     KGLGLFAETGNEAVARGSIAAGLGFFAGYPITPSSEVMETLIDELPAAGGRVVQAEDE
                     MAAMGMVLGASFGGVHAMTATSGPGLSLMTEMIGLSSMAELPAVVVDCQRAGPATGMP
                     SRTEQSDLFHAIYGGHGDFPRAALGIFDVVHGRDVMPRAFQIAEKYQLPVIVLSDAYI
                     AQRRQIRDPAVARRERPERERWTPENGLPARFDVTGEHGVNPFRVPGTPGGSYLAAGI
                     EHNQEGYPTADTSMHQRMNEKRFRKIAGIAAETRDWYRTLGDGKAPKGIVAWGSTYGL
                     LREWVIARPEWRVFLPEIIHPFPMEAFESWRRGLSSLAVVELNYQGQFHRYLSGLTDL
                     RGAKSVARSGGTPMTAAELDRLLEEAGS"
     gene            20125..>20674
                     /locus_tag="VFM00_09080"
     CDS             20125..>20674
                     /locus_tag="VFM00_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7498441.1"
                     /translation="MSTTKAKDYRSGLEPVWCTGCGDYGVLKGLTQALADLEIPSERL
                     AVISGIGCSSRLPGYTKSYAFNSIHGRALPIASGLKLSRPEITTVVVGGDGDGFSIGL
                     GHIPHAIRRNVDLTYAVLDNGIYGLTKGQASPTTAQEFKRDRGLAGVEERPLNPVLLV
                     LSSGCGFVARTHAGNLPHLRQMF"
BASE COUNT         2888 a         7185 c         7274 g         3327 t
ORIGIN      
        1 cgatgcaggt gcgcaccccg gtgcgctacc tggccaacgg cgtcgacatc gtaaacggcg
       61 tgttcgtcca gaacggctcc accctgcgtg gcgaagtgat ttcgccgatc ccgctgacca
      121 gcgactcgct gagcctgcgg atcgacggga tcccggtggc ggccacgatc accaagctgg
      181 acgcgccggg gcggcagtgg gcgctggagt acgtcgcgtc ggatctcttc acggggaccc
      241 acacgatcca gctcttcgtc ggagcccagg gattcgagca gcgcacgttc cagaccacca
      301 gcgagttcac gctccgcggc gtcgcggtgg tcgacccgcg gatgcagggg acgggatgcg
      361 gcggctcgat cttccagtac gagctgagcg cggcggcccg caaggtggag ctgcagctct
      421 tcaccgtcgc ggggcgccgc gtggcgtcga tcgatctgcc gggtcaggcc ggcttcaacc
      481 tcttctgctg ggacgggcgc gactcacgca cccacgacac cgcccagggc gtctacctct
      541 accgcctgcg cgccaccgac ccgaacggga agacggtgac gcgcgacggc cggatgatcc
      601 gagcccggtg accgaccgcc ggaacgggga tcgccggagt ggggggggcg ggggacgcgt
      661 ggcgcggggg gccgatcgcc ggagcggcaa ggccatcttc cgcgagctct cgaagctggt
      721 gacggagcag cgaaatccgc gctcgcgacg gctggaccgt ctcagcaccg cccagaccct
      781 tcgattgatg aacgccgagg accgacgcgt gccgctcgcg gtggggcgcg agatcccgaa
      841 gatcgcccga gccgtggacc tcatggtgga acgcctggag cggggcggcc gcatcttcta
      901 cgtgggcgcg gggaccagcg ggcggctcgg cgtgctcgac gccgccgaat gcccgcccac
      961 gttcggcacg ccgcgctcgc tcgtgcaggg gatcatcgcc ggcgggcgcc gcgcgctggt
     1021 gcgctcgatc gagggcgccg aggacgatgc cggcgccgcc gtggccgcgc tcaagaagaa
     1081 gcgcgtcgga cccaaggacg tcgtggtcgg catcatggcc agccggcgca cgccgtatgc
     1141 gatcggcgcc gtcgagcatg ggcgcaaggt cggcgccgcg acgatcgtgg tcaccgcgaa
     1201 tccctcgtcg gacgcgcggt tcgacgtgga cgtggtgatc gcgccgcgcg tgggaccgga
     1261 ggtcgtgacc ggctcgacgc gcatgaaggc gggcacggcg cagaagctgg tcttgaatat
     1321 gctctccacc gcgacgatgg tgcggatggg caaggtgtac gagaatctga tggtcgactt
     1381 gaagaccgcg agccggaagc tggaggagcg cacgaagcgc gtcttcatgt acgcgaccgg
     1441 cgcgcgctac gaggaagccg agcccgcgct gaagcaggcg ggaggctcgc tcaaggtggc
     1501 gatcgtcatg cgacgcgcca aggtcggccg ctccgaggcg gagcggcggt tgaagagggc
     1561 ccagggatgg gtgcgccagg cgatcgaaaa ttaatgtccc ggcggacgga tatccgccgg
     1621 aagacgcgaa gtaccgttca gcgcgtggcc gcgaccctca tcgcttcgat gcttgtcgtc
     1681 gcgtgcggcg cgggttgcgc gaaatcacgc tccggcaaga cgacggtcgt cttctggcag
     1741 ttctctcccc tcgcgacgat ccagccgatc ctggaccggt accgcgccga gaatcccggc
     1801 gtggacctgc aggtggagca gctcacctgg cagagcggcc gcgagaagat cgtggcggcg
     1861 atcgcggccg ggcgcccgcc cgatctctgc gagctgggct cgacgttcct gcccgggctg
     1921 gtcgccgaca gcaccctcgt cgatctgagc gattcgatcc ccgacctccg cgccgagctg
     1981 gtcggctggg acgccgtctc ctaccgcggc cgcgcgttcg cgctcccctg gatgctgggg
     2041 acgcgcgcgc tcttcatcaa cgaggatctc ttccggaagg cggggctgga tcccgcaaag
     2101 cctccggcga cgtgggcgga gctgctcgac gacgcgcgcc gcgtcacgca gcgcgtgccg
     2161 gatgccaagg gcttcggcat gaactccggc gagcgggaga tcctgatcaa gaagttcatg
     2221 cccttcgcct ggggaaacgg cggcgacatc ctcgactcga cgctcacgcg gtcggtcttc
     2281 aattcccccc agaacgtgga agcgctgaag ttctacctgt cgctcaagcc ctactcgctt
     2341 ctcgaccgcc aggagatgca cgagcaggcg ttcgccaagg ggcggctcgg gatcgtgatc
     2401 tcggggccgt ggctgttgcg cacgctggcc aagaccgcgc ccgacgccca cgtccaggtg
     2461 gggctgatgc cgcgtccggc cgcgaaccgc gggaagtccg cgtcgttcgc gggcgccgag
     2521 gtgctcggga tcttccgcgg gtccaagcat cgcgccgagg cgttgcgcct ggcccggttc
     2581 ctggttcagc cccgaaactc gatgccgctc tacgtcgcga cggggaacgc gttccccgcg
     2641 gccacgagcg ccgcggccga ttcctacttc acgacgcacc cgatggaccg gctctttctg
     2701 gaacagctcc acaccgcggt ctcgccgcct ttgcacccgc gatgggtgga gatcgaggag
     2761 atcgtgaacg ccgagctgga gcaggcgatc tacgggacca agacgcccgc ggccgcgctg
     2821 gccgaggggg acgcgaagat cgcgcaggtg ctggccgggc ccaagccgtg accggcgcgc
     2881 cgccggccgc gcccggggcg cgcggcgcgt gaggggaagc cgtccctcga tcgggctcct
     2941 cccctggggg cttgcgtttc tcgtcttcgg cctcttcccg cttctctacg cgctcgttct
     3001 ctccttccag cagatgaacc cgctgcgccc cgatctcacc catttcgtgg gcgtgtccaa
     3061 ttacgcccgc gcgctgacct cggcttcctt ctggcattcg ctccgaacga ccgtgttctt
     3121 cgtggtcggg acgattccgg tgacgctgac gctggcctat ctcgtcgcgg gccagctcgc
     3181 gacgatccgc cgcggccagg gcttcttccg cgcggcgatc ttctttcccg cgaccgtttc
     3241 gatggtggtc atcgcgttgg tgttcaagtc gctctacgcc gaggacgggc tcttgaatgg
     3301 ctggctggcg gcgtcggggg tgtcgcgcgt gcactggctg caggacgtgc gcaccgcgct
     3361 tccctcgatc atgctcatgg acatctgggg gagcgtcggc tactacgcca tcctgatcct
     3421 cgcgggacgg aagacgctgc cgcccgagca gctggaggcc gcgcgcctcg agggtctgtc
     3481 tgcctgggag gtggagcggc ggatcgtgtt cccccatttg cgcccggtgc tgttgttcgt
     3541 cgtgctgctc aacacgatcc gctccttcca gatcttcacc gaggtcttcg tgctcacgcg
     3601 cggcgggccg ctcgagagca cgctgaccct ggtctatcac ctgtacgagc gcgccttcta
     3661 tcacttcgag atgggctacg cgtcggcgat cgcgtacctg ctgctcgcgc tggtgggggt
     3721 ggtgctgctg gtgcagcggc gggtgcttat gtcgcggcgc gaagcgccgc tgagtggggg
     3781 ggtggggtga agcctcgccc gctcggcgcc tatgtgattc tctccgcgat cgcgcttctc
     3841 tcgttcgccc ccctgctgta catggcgcgc gtgtcgctag gacagggagg cgacctcccg
     3901 atctccccgt cggactggtg gggccggccg ttcactttgg atcactatcg cgcgctcgcc
     3961 gccgggggac cgatggcgcg gttcgcgatc aactcgatcg tcgtctcgtg cgtcgcgctg
     4021 ccgctccagc tcctcctggc cgcgtcggcg ggacatgcgc tcgcgcgccg ccgcttcctg
     4081 ggcaagaacg tgacgctcgc gctcgcgacc gccttcctgg tgctgccgcg ccaggtcacg
     4141 ctggtgccgc tctatctgtt gatggccaag ctggggctga tggacaccta tgccgcgctg
     4201 atcctgccgc acctggccga tcctttcggc gtgctcttca tggcgcacta ctatcagacc
     4261 atgtccgccg agctggagga cgccgcgcgc accgacgggc tctcggcggg ggcgatcttt
     4321 cgccgcatcg tgctgccgct ctccggcccc gggctcgcgg tcgtgggggt gaacggcttc
     4381 ctcacgacct ggaacgcttt ctaccagccg ctcgtgctca ccacgtccga gtcgatgcgc
     4441 acgctgccgg tggggctcgc gctgttcgcc cagtccgagt actcggtgga ctggggtgct
     4501 ttgctcgcgg gctcgtcgat cgccaccctt cccgtgctgg tcgcgttcgc gctcttccag
     4561 cggcagatcg tcgagggcgt cctgcagggg agcgggagat gaccgccgac aagcgcgggg
     4621 cgtggcggcc ggaaccggcc ctgatccgga aaacgacggc gaccgccggc acggtgcgcg
     4681 ccatcgggct catgagcggc acctcggccg acggcatgga cgcggccgcc gtgttgatcg
     4741 aaggaggcga cgcgccgcgc cgcgcgacga agctcgtcgc gctccggtcg gtgccgttcg
     4801 agcgggagct gcgcgccgag atcctcttcg cccaggaggg agagctgccg ctgcggcgcc
     4861 tgttcgcgct ggcggtcgcc ctcggcgagg tgggcgccga cgccgcccgc caggcgcacg
     4921 ctgccgccgc atggaacgag cctcccgacg tgatcggctt ccacggccag accgtctttc
     4981 acgatccgcg cggcgagcgc tcgggacgcc ccctcacgac gcagatcggg gagcccgcgg
     5041 tgatcgcgca cgcgctgggc gcgccggtcg tgtcgggctt ccggatggcc gacgtgctcg
     5101 aagggggcga gggcgctccg ctggtgccgc gattcgacta caaccagttc ggatccgaca
     5161 ccgccgatcg cgtgctcctg aacctgggcg gaatcgcgaa tctcacgcgc cttcccaacg
     5221 gcgctccgat cggcgccatc acggcgttcg attgcggacc ggcgaacatg ctcctcgacg
     5281 gcatcgtggc cgcgttgcgg cccgaggggc cggcctacga tgccggcggc gcgctcgcgg
     5341 ccgggggccg cgtggacgag gccgtggtcc gcgagttcct gggccatccc ttcttcgcgc
     5401 ggaagcctcc gaagagcgcg ggacgggagg agttcggcgc cgagttccgc gcgcgcttct
     5461 tgagccggac cgagggccgg tcgctggagg accggctgcg cacggcggtc gagatcacgg
     5521 ctgccggggt cgcgcgcggc gtggagcagt cgggaccgcg cgctccggac gagatcatcg
     5581 tgtccggagg gggtgccaag aacgttgtgt tgctcgatgc catccgcgcg cggttgccga
     5641 agagcgtcgt gcgcaccagc gacgagctcg gggtgccctc ggaggcgaag gaggcgatgg
     5701 cgttcgcgtt cctggccgcg gagacgatgg cgggacggcc gggcaacgtg cccgccgcga
     5761 ccggcgctcg gaaggaggtc gtgcttggca gcgtcacgat gccgcccgat cccaagcgct
     5821 agggcgccgc gccgctcgct cccaagcgct agcccgtcgc gccgcccgct cgggttgctc
     5881 gctgctgttt tcgtcgccgt cgcgctcgtc tcgggctgcg ccgcaaaccg cccccgcgag
     5941 gagtccgcgc cggttccatc ccgaggcaga cctgcgccca gcgaaccttc atcgggtggg
     6001 accagtccgg aacgaggcac cgcgagccgt gggacgccgt cgtcaccgcc cggtcccgcc
     6061 agccagggta tccccggagg cggaccgccg gcgccggggc ccgtgagcga cgagtggcgc
     6121 acgcgcgacg tcgtgcgcat cgggctcaag gtcggcgcgc cggacctgct tcttgccgga
     6181 tcgagcgcat ggaagctcga gacccccgac ggcaacgacc tcgcccacgc gggccccgga
     6241 gaagtgctcc ggatccgcca gcgaggcggc tcgatcgacg tcacgaagca gggcggcggc
     6301 gacgcgctcg ccagcacgga cccggacgac acgctcctgc tcgtgccgga gcgaggctac
     6361 gccggatgga gcggacgctg gtaccgcggc acgttccgcg tgtacgccac gccgcccggc
     6421 tcggacggca cgtcctccgg actgacgctc gtcaacgagg tgccgctcga ggactatctg
     6481 cgcggcgtcc tccccgagga gatcggaaac ccgcccgcga ccgacttcga ggcggtgaag
     6541 gcgcaggcgg tggcggcgcg cacgtacacg ctctcctacc tgagccggcg cgccgacctc
     6601 ggcttcgacc tctgggccac cgtcgaggac caggtctacg gcggcaccgc gcgcgagaac
     6661 gcgcaggcga gccgtgccgt ccaggagacc cggggcgaga tcctcctctc ggacggagtg
     6721 ccgatccgcg cgctctattc ctcggcttgc ggggggagga cgtcgaacgt ggaggacgtg
     6781 tggccctggc cgtggacgcc ctacttgagg agcgtacgcg acgccgagga cgagaagctg
     6841 acgggctact gctccctctc ggcgaactat cgctggagag aggagtggcc ggtggatcag
     6901 ttcgtggcga cgctccggaa gtacgcgccg gccgagggag cctcggtggc cgcgctcgat
     6961 ggtcagatcc tcgacgtgcg catcgagaag cgctcgcgca gcggccgggt ggccgagctg
     7021 gtggtggaga ccacggaagg tgaatgcgtg ctccacggcg accgtacgcg ctgggcgctg
     7081 cgccgccccg gcaccgaggc gatcctccgc agctccttct tcaagatcgg catccagcgc
     7141 gcggccgacg gcgcgccgct caaggtgatc gcgtcaggcg gcgggaacgg ccacgggatc
     7201 gggatgtgcc agtggggcgc catgggcatg gcccgcgcgg ggaagaacta ccgggagatc
     7261 ctgcggcact actacaagga cacgcagctc gcgcatctat aggaatcaca cgcgcccgat
     7321 cgtgaagttt tcacgtcggg cttcgccgtg gtaaacgaac gccgagcaac ttgcgccagc
     7381 gcaggttaat ctatatatgt aactaacacg cccgcaatag gttcgccccg cgctgatgac
     7441 atgttccaga acatgcgaca cgtgccgact tggctcaccg accccggtcg ggacttccga
     7501 catactctgg agtatgtcgc ttgtgtcagt tcgtctcgtg actggttagc tgcgtcctgg
     7561 cttatcaggt tgcattcgcg gtcgcgcgag tcgcacggga ttcctcggag ggctgccacc
     7621 gccagaaaaa gaggcggcgc ggctaccggc aacggcgtcc ttcccccaag gatcgccgcc
     7681 cggccggtgc cgcgccgctt tacctcgact tgcgctggcg caagttaatt tatatattaa
     7741 ctacttccgg cccccggcat caccgggccg tcaccgctct caatgcagcg tggccaggct
     7801 gtacagccgg tccacgttcg actgcgtcca ggtgcggagc cggtccacgg cctcctcctt
     7861 gccggggcgc ggcgcgaact tcccgtcggg tcccttctgg acgaggtggt ggaaccgccg
     7921 ctgaccgccg aggaactcct cgacggcggg gcgttccgtg atctcctggg gacaatcctt
     7981 caccgaaccg cccgcgccct tctcccagat gaaaacgggg aagacgccgc actgcgcgcc
     8041 cagcgtggcc agccggacgg tctcgccatc ctcgaacttc cacccgctga tgcagggggc
     8101 ggggacgaag atgacggcgg tttccttgca cgccagcgct ttctccatcg tcttcacgaa
     8161 gagcttgggg tgcgcggggc ttacctgcgc caccaggccc gcgcccgcgg cgatggccag
     8221 gtggatgtag tcgaggggag gcctcgggtt gcccaaggag gcctccccga ccggtgtcgt
     8281 gctggtctcc gcgaaagggg tcgtagccgg gctgtattgg aagcccgtgt tcgcgaagat
     8341 ctcattcacg agcaccatca acgtgacccg gctgcgccgg tagatcgtat gcaggaagga
     8401 gcggagcccg atggcgaagg cgccgccgtc gccgctggcg gcgatcacct ggaccggatg
     8461 ggcgaacgcg ccgtggtcgg ccagcaagtc ggccacgtcg cgcgcggcct cggccagcgt
     8521 gggcgcgctc tcgaagacgt tgtggatgat gccgaacgac ttgtggaaat cgtcgggcgg
     8581 cgtgtcgccg cggccccacg cgaccacgtt cgggaacgcg aggctggaga cctccgcgca
     8641 cgacgtgccc agcgtgaaga ccgtcttcat gccgttgtcc gagacgcgtc cgacgttctg
     8701 gagcgcgatc gactccatgc acccggggca gagcgtcgag ccggggcgga agcacgattc
     8761 gcgtagcgcg atttccttga acccgctcat agctgcgtcc cttcgtggaa gaaggcgacc
     8821 ggcggaaggg ccttgccgtc cagcgccgac cgcgcgtgat ccagaatccg ctcccaggtc
     8881 ggctcggaca cgtccgcgcc gcccagtccc gtgaacacgc tcacgatccg cggcggtttc
     8941 tcgatgccgg agagggcgtc ggcgacgtcc agcgtgaggt gtccgcgtcc ctggtggtgc
     9001 gcctggttca cgaccagcac caccttggcg ccggccagcc gcgcgcgcac cgcctcgcgg
     9061 gggaaaggcg tcaggagccg catcgcgatc cccgcgacgc gcgtccacgc cagcctctgg
     9121 tattcctcgc ggatcgcctc gaacgtgccg aagtcggggc ccatgctcac caccgcgatg
     9181 tccggcggcg tgccgttctg gaatccgacc gcatcgtagt aggcgagccc cggacgtccg
     9241 aactttcgct cgaaatccgc cccgatgatc gggaggatgt cgatcacgcg atccatgcgg
     9301 cgccgctgcg ccaccttgaa gccctggaaa tggcgcgacg tgacgcaatt gccgaacgcc
     9361 gagtcgccgt tcaggaggcc gggcaaagga cagctgcgga tccagtgatc ctggaacgag
     9421 tggatctccg cgtccggctc cacggtgagg cgcgcgttcc gatggctgtc cttgatgccg
     9481 tagtagccgg gcatcgtcgg cgtcatgatg tcgggctgca tgcccaggca gggcgcctgg
     9541 agcaccgtgt cgtagatctg ctgcttgccg cggcaggcca gctggatgaa tccgtcgtcg
     9601 cgatgggcca gcgtgtcgct ggggtcgcct tcgatgcaga gcgggaagtt cgccgtcgcg
     9661 cgatagacgt tcaccagcat cacgttccct agtccgctcg cgcccagcga gcgcgtggtc
     9721 tcggtcatgt ggtccagtcc cacgctgctc gtcgcggtgg ggaagatcag gtcgcggcag
     9781 gcggccgcgg cgccgaccaa gtagtcggcc acggcgtgct cggcctcgag cagcttcacc
     9841 cgcttcttcc cgcccacgtc ggccgcgacg cgcgcggcca cggtctcgat ccacttggtc
     9901 gagggggtga tcgggaatcc cgcgaagagg atgcagcgcc acatctggag cgcggcgatc
     9961 gcggcggcgg tgttgccgtc ggcggtgatc gtctcgcgct tggtgtagag cgcgtgatgc
    10021 gcggcccgca ccttggcgag gtcgatcgcg tcggggtaga tctccccgga cggggtcgcg
    10081 cagtgggtca gggcggcggt cttctcggag gccttgctca cgctcttctc cggggccttg
    10141 ccgttcttct cgaggacttt gctcatgacc ggctcacctc catccagggc tcttcataag
    10201 ggacttccag gaagaggtgc tcggggcaga cggcgatgca ttcgccgcac agcttgcaga
    10261 acgtgctcac gtccacgccg gtcacgagca gcccgcgctc cgcgtccgga acgaagcgga
    10321 tgatcccctc ggggcagttc gtgatgcagt gggcgcagcc gttgcacagc gtcttgggat
    10381 cgtcgaagcg aagtcggaga ccgctcggcg cgtagttggc cgtctggttt gcggccgaca
    10441 gccggagcgc cgtttgcgcc cccaccggaa ggtagccgta ccccagccag ggcgcactgc
    10501 tgcggcgcgt ggattcctcc tcgaacacgc cctcgcgaac cgcctccagc gaggcgtgga
    10561 gcgcgccgcg gttccgttcg accaccgcgt cggggacgtg ccgcttcttc agggtctcga
    10621 ggaaggattc gacgatggct ttttcctcga atcctccaat cgcgtgcgcg acggcgacgt
    10681 agatcgagac gttgccgatg gcgtgcttca ggtgctcgga gccgatggcg tcgccggcga
    10741 tcgtcacgac ccggccggtc agcccgtaat gcagcgcgca ctcttcgggg gtgtgcggcg
    10801 tgttgagcac gaacgttccc ccgtggggca ccccctgcgt gaagtcggcc gaccgggccg
    10861 caccctcgtc catcaggacc gagagcgccg gtcgggtcac ctcacaggcc gccagaatcg
    10921 gcttggcgct gacccggagg aaggagcgga tgttcgcccc ccgccgggcg ctgctgaaga
    10981 agggccactc ctggacgtgc agccccgagt cccggagcat gaggttggcg aggctctgga
    11041 aggcgaggac gaggccgccc ccggccttgc cgtccccccg aacgtccagc accgaagtcc
    11101 ggattcgctc cctcagatcg atggggggca ttcgtatacc tactttttcc gccacagaat
    11161 ggcgtgcccc gggcactcgg cgatgatctt gtcgagcttg ggcttttggg actcatccac
    11221 ggccgtgttg tagacctcgg ccacttcctc gtcctcgacc ttgcggaatg ccaagggcat
    11281 cgccttgata cagtccccgc attcggtgca caggtcttcg tcgatgtaga actccggtac
    11341 gttcgccatg ggggtcaccc tccttcaggg atggcagctg cattttcgtc tgttccaaat
    11401 gtcgcacgtt gcccccggat tctccctggt actttggcac cgctaccttg gtagcggggc
    11461 accgctactt tggtagcagg ggatttatgc gggcaaaatc gtagactccg gtcgataggt
    11521 cttcctgaca aaaggtcagg ttgcactcca accgaactgg agcaagtcat ggccgaccct
    11581 acgaagccca ccaagcgctc gggaaccgag gatccccagt tccaggccgg ccgccggcgc
    11641 ttcctgggcc tggcggcgct cctgtcgggc ggcgcggccc tggccgtcgt gcgtccgaac
    11701 tcgatcccgg ggctgggaag cctcacggga accccggcgc gcgtccgccg cctcgagtcc
    11761 tcccgcccgg cgctggggac ctggatgcga gtggtcgtga acgaccacga cccgaagcgg
    11821 gccgagcgcg ccatcgaggg cgccttccgc gcggtgcgcc tcgtggatgg gcagatgagc
    11881 atccaccgcc ccgagagcca ggtgagccgc gtgaacgccg ccgccggacg cggcagcgtc
    11941 cgcgtggacc gggcgctcct ggacgtggtc gaccgcgcga ccgccatggc gcgccgcacg
    12001 aacggcctct acgaccccac cgtgctcccc ctgatgaagc tctacggctt ctatcgctcg
    12061 gggcggacgg cctggcccag cgatcgcgag atcgcggcca ccctcgaccg gaccggatgg
    12121 cgccatgtgc gtctgaaccg cgcggcgggg accttgggcc tggcccgcga tggcgtcgcc
    12181 ctggatctgg gctcgatcgg gaagggctgg gcgctcgacc gggccgtgga cgcgatccgc
    12241 gccgaggggg tcacgtccgc gctggtggat ctgggcggca acatctacgc gctcggtgcg
    12301 cccgacgacg acgcggcggg ctggtcggtg gggatcccgc acccggagtc gggcaagatc
    12361 gaacgcgtgc tcacccttcg cgatcgcgcc gtcgcgacga gcaacgaccg ggaacagcat
    12421 caccgcctcg gcaggttcga gatcggccat cacctggacg cgcgccgcgg ccgcccctcg
    12481 ggaggccaca cgagcgcgac cgtggtcgcg aagaccggcc tcgaatcgga cgtccactcc
    12541 agcgtcgcct atctcctggg cccctcggga ttccacggat tctccgagat ccaggagagc
    12601 ttcttcctgg gatagctcat gcccgagctc aagacattcg gccgcggggg catccacccc
    12661 gatccccaca agcgggacac cgccgagcgc gggatcgagc cgatcgatcc gccgccgaac
    12721 gaggtctggc ttccgctgct gcagcacctg ggcgatccct ccgttccgat cgtgaagaag
    12781 aacgatcgcg tcgtgcgcgg gcagaagatc gccgaggccg gaccgagcgg cgttccgctg
    12841 cacgcgacga tctcgggaaa ggtgaagccg atcgacaagc gcccgcaccc cacgctggtc
    12901 tcctcggagg cgatcgtgat cgtgcgcgag agcgacgagg agctggagtt ccccgaggat
    12961 ccgggctggc gcgagctggg cccgggagcc gcgctcgagc gcatccgcga cgccggcatc
    13021 gtcggcctgg ggggtgcggc cttcccgacc tatcgcaagc tcacgctgcc cgaaggagtg
    13081 aacgtcgaca cgttcgtgct gaacggcgcg gagtgcgagc cctacctgac ctccgactac
    13141 cgcctcatgc tgtccgaggc ggaggcgatc gtcgagggcg cgctcgcgat ggcgcgcgtc
    13201 gtcggcgtgc accgcgtgct cgcgggcgtc gaggcggaca agatgccggc ggtcctggcc
    13261 ctgcgcgagg ccgcgaagcg cgtcccgcac gagggctgct ccttcgacgt ggtgccgtgc
    13321 gaagtccgct atccgcaggg ggcggagcgc cagctggtgg ccgcgctcac cggccgcgcc
    13381 atcccgccgc gcgccctccc cctcgcggtc ggcgtcgtcg tccagaacgt cgccacggcc
    13441 catgccgtct atcaggcgct gcgctaccgg aagccgctcc tcgaccggat cgtcacggtg
    13501 acgggatccg ggatcgtcta cccgcggaac gtccgcgtgc cgctggggac gctcctcgaa
    13561 cgggtggtcg atttctgcgg cgggctccgc gtggatacca cgcgcctgat cgcgggcggg
    13621 ccgatgatgg gacgcgcgct cccgcggctc gatcttccga tcacgaaggg gatgaacgga
    13681 cttttggccc tcaccgggcg cgggcccttc gagatgggct atggcccctg catccagtgc
    13741 ggccgctgcg tggacgcctg cccgctgggc ctcgagcccg accaggtctc ggtgcgggtc
    13801 gaggcgggga aggtgctcga caccgagccc ttcggcgcgc gcgaatgtta cgagtgcggc
    13861 tgctgcacgt acgtctgccc ctcggggcgg ccgctcctcc agttcatgca ggttgcgaag
    13921 tcgtccctgc ggcgcgcgac cagcgtgcgg gtgaccgcat gagcgaaccg gcgatcgcga
    13981 ccgctcaggg acccttcgtc ctcgccccgg gtccgcacgt ccggacctcg gaatcgacgg
    14041 cgtcgatcat gtggaccgtg aacgcggcgc tcgttcccgc ggcggcgtgg ggtgctttga
    14101 tgttcggctt cccggcgctg gcggtgatcg gggcctcgat cctcggcgcg gtggccgccg
    14161 agtggggtgc cggccgggtc atgcgcgcgc gcacgtcggt ccgcgacggg agcgcggtct
    14221 gcaccggtct tctcctcgcg atgacgctgc cgccccggct tcccgcgccg gccgctttcc
    14281 tcggcgccgt cttcgcgatc ctcttcggga agatgatctt cggcgggctg ggctacaacc
    14341 tgttcaaccc ggcgctcatc gggcgcgcgt tcctcatggc gacgttcccg cttccgatga
    14401 cggcgggctg gtcgcccccc gcgaagtgga tctcgcacgt ggacgcggtg acgacggcga
    14461 cgccgctcgc cgcgctgcgc gagcgcggga tcgacgccgc gctcaagctg gcgggcgggc
    14521 ccgaatcgcc gctcgcggga ctcttcctgg ggatccgccc cggatcgatc ggggaagtct
    14581 cggtgcttct cctcctggtg gggggcgcgg tgctgctcct gcgccggatc atctcgctca
    14641 ccatcccgct cgcggtcgtc gccggcgtcg ccctggtgac cgccccgacc gggcacgtgg
    14701 atctgcatct tctctcgggc ggactctggc tcggcgcgct cttcatggcg accgactacg
    14761 tgacctcgcc cacgacgcga aacggccaga tcgtgttcgg cctggtgatc ggcggcctga
    14821 ccggcgtgat ccggatctat ggcggctatc ccgaggggat ctgctacgcg atcctcctcg
    14881 cgaacgcgct cgtgcccgcg ctgaatctct ggttcaagcc ggcgcggccg gtgttcgcgg
    14941 gggcgccgtc gtgagcggcc cggacgtgaa tgctggcggc cccatggccc ccgggggcgg
    15001 ctccggtgga ccgggcaagg ccccgagccc ggccggcgag gtggtccgga tcgcgtcttc
    15061 gatggcggcg gtgtgcgccg tgggcgcgct gatcctgggc ggcgtctatc tcgcgaccga
    15121 acgctacgcc gaggcggcgc ggaagacgag cgagcgcgaa gcggtgacgc agatgctggg
    15181 gctggacgcg cgcgccaccg tgctcgaggt gagccagtat ctcagtccga acggggacga
    15241 ggtcgtgtac cgcacggcgt ccgagggtgc cgtgcccggc cgcgagctgg tcttttcgct
    15301 cgacggcgcg ctgctcaaga acggcagcgc gccgctcgcc cccgcgccgc gcgagctccg
    15361 cccgttgggc cgcgtcttcg ccgcgcggcg gaacggcgcc cccgcgggct tcctcgtcga
    15421 gggggaagcc cgggggtaca agaaccggat ccgcttcttc gtcgcgctgg acacggcgtg
    15481 gacggtgcag ggcgtccgcg tggtggagca cgaggaagat cccgggctgg gcgccgaggt
    15541 ggccacgccc tggttccagg ggcagtacgc cgggcgcacg ctcgccgacg taaccgggct
    15601 ggacgtgacc aaggacccga tgcccgagga ctggcgcggc gcgctccgcg acctgggccg
    15661 gctgaccccg accgcctggc aggaggcgca cggcgcccag cgcgagcggg agcggaagca
    15721 tccgatctac gccgtcaccg gggccacgat ctcgagccgc gcgatcacgc tgggcgtgcg
    15781 cgcgaccatc ctccacttcc ggaagcgctg ggagcggatc gctccctatc tcgaaaccga
    15841 cgtccgcgag gcctcgcgat gagcgaacaa ccccttctct tcacccagcg cccgcccggc
    15901 gcggtctcgc tcttgaagaa cggcatcttc ggcgagaacc cggtcttccg cctggcgctc
    15961 tccctctgtc cggccgtggc cgtcacgacc accgtcgcga acggcatcat gctcggaatc
    16021 gcggtgctcc tcgtgcaggt gctctccagc gtgaccgtgg cgctcacgcg gcgctggatc
    16081 cacccccggg tgcgcatccc ggtctacacg atcatcatcg cggtctgggt aagcgcgatc
    16141 gacatggtgc tcgccggctt cttcccgctc ctctacgcgc aggtcggcct ctacgtgaag
    16201 ctgatcgtgg cgttcgcgat catcatctcg cggctcgagg tgttcgcctc ccgcatgccg
    16261 ctcatcccca ccttctggga cgggctgggg atggggctcg gcttcctctt cgcgctgatg
    16321 gcgatcggga ccttccggga agtgctcggc aacggaacgc tgctcgggtt ccgcgtcgtg
    16381 gcggaaaagc cgcttctctt cttcgcgctg cccgcgggag ggttcttctc gatcgcgctc
    16441 ctcatggctt tcttcaacgt gctggagcgc cgcgcgtcgg gcggtaagac cgcggcgccg
    16501 tccacgggag ggagccatgg ccaagccgcg tagcctcgtc ctcgccggga ccggcgccct
    16561 ggccctctgc ttcgcgatcg ggctcgcgct ggccgcggcg gccgggcgcc cggccgcgcc
    16621 ccacgcgccc tcgggtgtca tcacccgtgc tcgcgtcctc gcgcccgatc acgtgcgcct
    16681 caccgtcgcc gcgcccgtgg ccgacctccg gccgtccaat ttccgcgtga ccgacgagcg
    16741 cgcccccgac gtgccgatgg acgtcaccgc ggtcgagacg cttcccgacg gggcgatcct
    16801 cctcacggtg gcggaccggc tggacccggc ggcgggctac cggatctcga tccagagccc
    16861 ggccgggaca cgcgaggcga agcccgcgcc gtgggcgctc ctcttcacgg tggtgatctc
    16921 ctcggcgctc atcaacaact tcgtcttcac gcgctacctc gggctctgca tcttcttcgg
    16981 cgtctcgaag aagcgcgaca cctcggtggg catgggcttc acgttcgcgg cggtcatgct
    17041 cctctccgcg gcgatctcgt ggggcctcta caccttcgtg atgcgccccc tcgagctcaa
    17101 gtttctccag gtgatcgtgt tcatcggcgt cgtggcgttc atcgtgcagg cgctcgacac
    17161 gatcttgcgg aagacgcacg cgtcgctgca ccagcgcttc ggcgtctacc tggtgctgat
    17221 cacgacgaac tgcatcatcc tggcggtgcc gctgctgaac gcccaggcgg aatcgggcgt
    17281 cggcgagtcg ctcgcgctgg ccctgggctc gggcgccggg tttgcgctcg cgctcttcct
    17341 catgtcctgc gcccgcgagc ggctggagct ggcgcgcgtc ccggtcgctt tccaggggct
    17401 cccgatcgcc ttcgcgctgg cgggtctctt cgcgctcgcg ttcatgggct tctcgggact
    17461 caccttcctc cggtgacgcc atgaccgacc tcctccacgt cgcgctctgg ggcttcgcgg
    17521 tcttcacggc gatcggcgtc gtgttcggcc tgacgctggc cggcgtggcg ctccgcttcc
    17581 aggtgccggt gaacccgacc gtggagaagg tgcgcgcgtt cctgccctcc gccaactgcg
    17641 gcgcctgcgg ctttgccggc tgccaggcct acgccgaggc ggtcgtgctg cggaacgagg
    17701 tctcgccgaa cctctgcgtt cccggccgcg ccgccgtggc caccgcgctc gcccgcctca
    17761 cggggaagga gctgggctcg gtcgaggatc gcatcgtcgt gatgcggtgc caggggacca
    17821 gcgcctacgc gcgaaaagag gcggagtacg ccgggctcaa cacctgcgcg gcggccgcgc
    17881 tggtcttcgg cggcccgcgc gcctgcaaga acggctgcct gggcctgggg gactgcgtga
    17941 aggcgtgccc cttcgacgcg ctctcgatcg gcatcgacgg aatcgccgtg gtgaacgccg
    18001 accgctgcac cggctgcggg ctctgcgtgc ccgtctgccc gaaggagctc ttccagttct
    18061 atccgcggac gcgacggatc gagctctcct gcgtcgccaa ggagaagcag tcggtcgtcc
    18121 gggccacctg catggtgggc tgcaccctct gccgcaagtg cgtggccaag tgccccgccc
    18181 aggcgatcga gtgggatggc cgcaccatca tcattcacca cgaaccctgc ctcgcctacg
    18241 gaccgtcctg cggcgaggcc tgcgtcgaca tctgcccgag cacgatcctg catcgcgtgg
    18301 gacagctcgc gcagcccgag ccggccgagc cggcgatgga ggcatgaccg agatgcgcgc
    18361 tccgacgacc cccgacacgc acccttccac gcgctcggag gcccgctccg gcgcgccgcc
    18421 gcgaccgcgg tcgctcgtcc ttgctctcgt ggggagcggc ggcgacggcg tggcgctcct
    18481 gggcgatctc ctgctcggca tggcggcgcg cgaggggctg tacggcatga tggtgcagtc
    18541 gtacgggccg cagatccggg gcggcgagtc ggccgccatc ctgcgcctcg ccgaccatga
    18601 ggtgcagtac gagggggacc aggtggacat cctcctctgc ttccgcctcc gcgacctggc
    18661 ccgcttccag agctcgctcc gcgtgcatcc ggggagcatc gtggtgatcg atcagggaga
    18721 gacggcggag gtgccggcct ggctgggcgc gacagccgac aaaccctacc gcttcccctt
    18781 cgcgcgctac gaagagggcg tcgaggtcga cggtccgccc aagaacatgc tcgggctggg
    18841 gctcctctgc cgcatgctcg gctggcccga caccctggcg caggacatcc tccgccagcg
    18901 cttctccgcg cgccgcgagc gcctgcagtc gaatctcgag tcgctcgcga gcggctacgc
    18961 ggtggtggac ccgccgtcgc gtccggcccc cgaagggaag gggctcgggt tgttcgccga
    19021 gaccggaaac gaggcggtgg cccgcggatc gatcgcggcg ggcctcggct tcttcgcagg
    19081 ctacccgatc accccttcat cggaggtgat ggagacgctg atcgacgagc tgccggccgc
    19141 cggcggacgc gtggtgcagg cggaggacga gatggcggcg atggggatgg tgctgggcgc
    19201 ctcgttcggc ggcgtgcacg ccatgacggc gacgagcggt cccgggctct ccctgatgac
    19261 cgagatgatc ggtctctcga gcatggccga gctgcccgcg gtggtcgtgg actgccagcg
    19321 cgccggcccc gcgaccggga tgccctcgcg caccgagcag tcggacctgt tccacgccat
    19381 ctacggcggc catggcgact tcccgcgcgc ggcgctcggc atcttcgacg tcgtgcacgg
    19441 gcgggacgtc atgccgcgcg cgtttcagat cgccgagaag taccagctcc cggtgatcgt
    19501 gctctccgat gcctacatcg cgcagcgccg ccagatccgc gatccggcgg tggcgcggcg
    19561 cgagcgtccc gagcgcgagc ggtggacgcc cgagaacggc ctgccggcgc gcttcgacgt
    19621 gaccggcgag cacggcgtga atcccttccg cgtgccggga acgccgggcg gcagctatct
    19681 cgccgccggc atcgagcaca accaggaagg ctatcccacc gcggacacgt cgatgcacca
    19741 gcggatgaac gagaagcgct tccggaagat cgcgggaatc gccgccgaga cgcgcgactg
    19801 gtatcgcacg ctcggcgatg ggaaggcgcc caaggggatc gtggcatggg gcagcacgta
    19861 cggcctcctg cgcgagtggg tgatcgcgcg gcccgagtgg cgcgtgttcc tgcccgagat
    19921 catccatccc ttcccgatgg aggcgttcga gtcgtggcgc cgggggctct cgagcctcgc
    19981 cgtcgtggag ctgaactacc aggggcaatt ccaccgctac ctgtcggggc tgaccgacct
    20041 gcgcggcgcc aagtcggtgg cgcggagcgg cggcacgccc atgacggcgg cggagctgga
    20101 tcggctcctc gaggaggcgg gatcatgagc acgacgaaag caaaggacta tcgaagcggc
    20161 ctcgagccgg tgtggtgcac ggggtgcggt gactacggcg tgctgaaggg gctgacgcag
    20221 gcgctggccg atctcgagat cccgtccgaa cggctcgccg tgatctcggg aatcggctgc
    20281 tccagccggc ttcccggcta cacgaagagc tacgccttca actccatcca cgggcgcgcg
    20341 ctcccgatcg cgagcgggct caagctgtcg cggccggaga tcacgaccgt ggtggtcgga
    20401 ggggacggcg acgggttctc catcgggctc ggccacatcc cccacgcgat ccggcgcaac
    20461 gtggacctga cgtacgccgt gctcgacaac gggatctacg gattgaccaa ggggcaggcc
    20521 tcgcccacga cggcgcagga gttcaagcgc gaccgcggtc tggcaggcgt ggaggagcgg
    20581 cccctgaatc ccgtgctcct ggtgctgagc agcgggtgcg ggttcgtggc gcgcacgcat
    20641 gccggaaacc tcccgcacct gcgccagatg ttcc
//
LOCUS       DASSLZ010000242         8806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_847980, whole genome shotgun sequence.
ACCESSION   DASSLZ010000242 DASSLZ010000000
VERSION     DASSLZ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8806
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847980"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFM00_09085"
     CDS             complement(<1..191)
                     /locus_tag="VFM00_09085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7498442.1"
                     /translation="MIPEPVALIVTVRNEEASIDALLDSLLGGSRAPDEIVVADGGST
                     DRTVERLRHRAASDPRVRFL"
     gene            complement(194..2962)
                     /locus_tag="VFM00_09090"
     CDS             complement(194..2962)
                     /locus_tag="VFM00_09090"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET7498443.1"
                     /translation="MRFAPIALTAAAAALIAAAVLGPAGPAAAAPNLIRTLPNKMTVI
                     VRENRSRPLATVQVWIDAGSRDETRSERGAASALSYLPYEETSTKQRGEVRKEAESFG
                     GTLGSESGYSSIIYNMTVPARYVNRALGILSDAVLHPSFNQYTLEQSVAKARGESRGA
                     LSAAGGVSLNALRESLYGDTPPGTPFHAPELEVAALTLPIVQRYYKENFVADRITVVV
                     VGDVEADAVADQIAAAFKDVPAGKSRPHPRVQAPKELKDPIVRSVPSPPDADGSVVAA
                     GFRAPLWGTADATALDVLLAVLVDAPGSRLERRLREGGNDFGAAAAQRSFGPDPGLVA
                     LVLSADPGRMADAEGILAQEIEKVRSQPVTAEECQVAIHSVLARELMSRSELWGLGRA
                     TALAYYQGHPGADEVYAQRVSAVRPEDLSGVARKYLDWKNGAVIEMMPARTADSLGTA
                     KDFPKRFQEKLSLYQGTYRSGPLATASTDEARRARIDGPLASIASTPFDAGRGRVERA
                     KLAGGIRLLTGPDHSAPGVTVAVYLNGGVRWENDKNNGITSLVRETLLNSNDPRRNGL
                     AYRQSLSLLGSFTPYQDRDMWGVSLAVPSSDWKEALNRLGAMMSHPDLDSVTVDATRI
                     LLLTALDKWQDDDAAQRQRLIFATKYAVSGYRLPGVGNKVNLISLPTSAIGAYYKKFV
                     VKPNLYVAVFGDVNPSEVGPAVEEAFRDVSAGPFHPGEPPVDLPFPGFREKWELGAGS
                     MTTIQLAFNGPPADSPEMPVFYVVNSLLSGPYGWFQKYVMAEPTAQSANSIVAQAIDE
                     SPIIASLTIGGPVQEEAMVKLLFRQFKKVAGMELIGPDLDQDLRNAKTHAIGTFYATF
                     TTNTSRAFQWARADLFGLPPDALLALPSKMDAVTPGDLLRVGTRYFQRGDWEKAPYAI
                     AETRPGGW"
     gene            complement(3104..3316)
                     /locus_tag="VFM00_09095"
     CDS             complement(3104..3316)
                     /locus_tag="VFM00_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET7498444.1"
                     /translation="MRHRGSVKWFSESKGYGFIQEDSGEEFFVHHSAIVADGFRTLNE
                     GEVVEFDPMEDQNRGKQATNVVRVQH"
     gene            complement(3387..3713)
                     /locus_tag="VFM00_09100"
     CDS             complement(3387..3713)
                     /locus_tag="VFM00_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HET7498445.1"
                     /translation="MADVDKVIKCRDCGEEFVFTVGEQNFYKDRGFQHEPTRCRRCRD
                     EKKRASGGPVSAAPISNAAPREFHEAVCSSCGQMTQVPFRPTPGKPVYCRDCFQNVRS
                     TRGMNV"
     gene            3991..5625
                     /locus_tag="VFM00_09105"
     CDS             3991..5625
                     /locus_tag="VFM00_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HET7498446.1"
                     /translation="MFALAAAALIGPARPGAAAPEPPPRPSADSLVVLHSNDVHSHLL
                     PFHRSEATLVGGAAARAALIERERERDPDLLLLDAGDIVQGTPAYNLFRGVPDTRAMS
                     AMRYDAVALGNHDLDDGPAAWLALRPLAEYPVLSANVFAAADSSWASGLGDAPSAEVR
                     RGARWIGGASVSSSASLRFLAKPYLIRSWHGKRIAILGLTTGSLVNIVRISANRGVAV
                     SDPVLVARWFVPILRQQADYVIALTHLGVDADQDLARRVPGIDLIVGGHSHTRLDEPV
                     FAGNGTAPGLAGVPIVQTGAWGDRMGRSVLYLANGRPSGFSYELLRVRPQDGEDPAIA
                     ALLRPVADSVSVAMGATVYESTGRVPTPQRDDVETAIGNFAAEVLRETAGAEVGIMNT
                     GGIRAPIPRGPVTVADVYSTFPFDNTIVVVPMRGDDLRRLLDFAARRIGKSDFAQVSG
                     VRFSISGDYASDIRVNGHPLDEDRIYRVATIDFLYEGGAGYTQFRKAGPAEPTGVFQN
                     EAAVSFLRRHPQYQFRTDGRIAWEGAARGLRSLQTR"
     gene            5997..6200
                     /locus_tag="VFM00_09110"
     CDS             5997..6200
                     /locus_tag="VFM00_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7498447.1"
                     /translation="MRKRGTVKWFNEAKGFGFIQQAGGEDVFVHYSAIQGEGFKTLSE
                     GQVVEFEIVEGPKGKQAANVTPV"
     gene            6294..8363
                     /locus_tag="VFM00_09115"
     CDS             6294..8363
                     /locus_tag="VFM00_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7498448.1"
                     /translation="MPEQPHQEEPDPGHQIPALPPDPPAGVIVDPAPIRFAKRLFAAV
                     IGPARAFTDPALFHKISLAAFLAWVGLGADGLSSSAYGPEAAWLALEGNVFLAIPLAL
                     LTAFTVFVIAASYQRIIERFPAGGGGYVVATKLLGPGAGLASGSALLVDYVLTVAISL
                     ASCSEQVFSVLPTQYQFVKLPFTFLLLGVLTLLNLRGVRESVAIISPIFLLFIVTHAA
                     GLVTGIVSNWGQVPLEWTHLHEQSSVAAQNIGLVGLVMILFRAFTLGGGTYTGIEAVS
                     NGVPMLREPKVETAKITMRYMAISLALIAGGILFCYLLYQVRPVPGRTLNAVLFSAIA
                     GTVFPPGSGLGHALVAITLISSAALLFVAAQTGFLGGPRVLVYMAFDRWVPSRFSSLS
                     ERLVTSNGVLFMAISAAIVLAATQGAVHILVVLYSINVFLTFSLAQLGMCRDALNLKR
                     EGQPWKRAFFISSLGFVVTGLLLIGTVYFKFTEGGWATLLVTAIIAMVCVMIRGHYVR
                     TNQSLHRLDQALTTVPLPASEPTQAPLKKNANTAVLTVTSFGGLGIHTLLNLFRVFPG
                     QFKQVIFISVGAIDSGQFKGAEEIEALRASTEAELRKYVEFARKLGIPAESRLALGTD
                     IVDELEQLCILVQEQFPRSVTFAGQLVFQKEGSIVRWLHNQTCVALQRRLAFHGLPMV
                     ILPVRVY"
     gene            8395..8721
                     /locus_tag="VFM00_09120"
     CDS             8395..8721
                     /locus_tag="VFM00_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017870066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HET7498449.1"
                     /translation="MSRSWKERAAAVRLEGHALLLAARDPRTPRLARALVWSLAAYAL
                     SPIDLIPDFIPILGHLDDVVIVGAGLWIARRMIPEAVLSECRERARSKAAEGKDPGKD
                     RGGAQG"
BASE COUNT         1320 a         3028 c         2999 g         1459 t
ORIGIN      
        1 aggaagcgta cgcgcggatc cgatgccgcg cggtgccgga gacgctccac cgtccggtcg
       61 gtcgagccgc cgtcggcgac cacgatctcg tcgggagcgc gtgatccgcc cagaagcgaa
      121 tcgaggagcg cgtcgatcga cgcctcctcg ttccgcaccg tgacgatcag ggcgacgggc
      181 tctggaatca accctaccag ccgccgggcc gcgtctccgc gatggcgtac ggcgccttct
      241 cccagtctcc gcgctggaag tagcgcgtcc cgacccgcag gagatcgccc ggagtcaccg
      301 cgtccatctt ggaagggagc gccagcagcg cgtcgggcgg aagcccgaac aggtcggcgc
      361 gagcccactg gaaggcgcgg gacgtgttcg tggtgaacgt cgcgtagaag gtcccgatgg
      421 catgcgtctt cgcgttccgg agatcctgat cgagatccgg cccgatcagc tccatccccg
      481 cgaccttctt gaactgccgg aagagcagct tgaccatggc ctcttcctga accggaccgc
      541 cgatcgtgag cgaggcgatg atcggcgact cgtcgatcgc ctgcgccacg atcgagttcg
      601 cgctctgggc ggtaggctcg gccatgacgt acttctggaa ccagccgtac ggtcccgaga
      661 gaagcgagtt cacgacgtag aagaccggca tctccgggct gtccgcgggc gggccgttga
      721 aggcgagctg gatggtcgtc atcgaacccg cgcccagctc ccacttttcg cggaagccgg
      781 ggaacggcag gtcgaccggc ggctcgcccg ggtggaacgg acccgcgctc acgtcgcgga
      841 acgcctcctc caccgcgggc ccgacctcgg aggggttcac gtcgccgaag acggcaacgt
      901 acaggttcgg cttcacgacg aacttcttgt aataggctcc gatcgcggag gtcgggagcg
      961 agatgaggtt gaccttgttc cctacgccgg ggagccggta gccgctcacc gcgtacttgg
     1021 tcgcgaagat gagacgctgc cgctgcgccg cgtcgtcgtc ctgccacttg tcgagcgccg
     1081 tgaggagcag gatccgcgtg gcgtccaccg tcaccgagtc caggtccgga tgggacatca
     1141 tcgcgcccag ccggttcagc gcttccttcc aatcggacga aggcaccgcg agggagaccc
     1201 cccacatgtc gcgatcctgg tagggcgtga acgatcccag gagcgagagc gactgccggt
     1261 aggccaggcc gttccgcctg gggtcgttcg aattgaggag cgtctcgcgg accagcgacg
     1321 tgatcccgtt gttcttgtcg ttctcccagc gcacgccgcc gttcaggtag acggccaccg
     1381 tcacgcccgg ggcgctgtga tccggcccgg tcaggagccg gatcccgccg gcgagcttgg
     1441 cgcgctccac gcggccgcgc ccggcgtcga aaggcgtcga agcgatcgac gcgagcggcc
     1501 cgtcgatgcg cgcgcggcgc gcctcgtcgg tgctggcggt cgcgagcggg ccgctccggt
     1561 acgtgccctg gtacagcgag agcttctcct ggaaccgctt cgggaagtct ttcgcggtgc
     1621 ccaggctgtc ggcggtgcgc gccggcatca tctcgatgac cgcgccgttc ttccagtcga
     1681 ggtacttgcg cgcgaccccg gagaggtcct cggggcgcac cgcgctcacc cgctgcgcgt
     1741 acacctcgtc ggcgccgggg tgtccctggt aataggcgag agcggtggcg cgccccagtc
     1801 cccagagctc cgagcgcgac atcagctcgc gggcgaggac ggagtggatc gcgacctggc
     1861 actcctcagc cgtgaccggc tgcgagcgga ccttctcgat ctcctgggcc aggatcccct
     1921 ccgcatcggc catccgcccg gggtccgccg agaggaccag agccacaagc cccggatcgg
     1981 ggccgaacga gcgctgggcc gcggccgctc cgaaatcgtt ccctccctcg cgcaaccggc
     2041 gctccagccg cgagccggga gcgtcgacta agaccgcgag caggacgtcg agcgcggtcg
     2101 cgtcggcggt gccccagagc ggcgcgcgga aaccggcggc cacgacggag ccgtcggcgt
     2161 cgggcggact gggaacggaa cgcacgatcg ggtccttgag ctccttgggc gcctggacgc
     2221 gcggatgggg gcgcgacttg ccggcgggca catccttgaa ggccgccgcg atctgatcgg
     2281 ccaccgcgtc ggcctcgacg tcgccgacca cgaccaccgt gatcctgtcg gcgacgaagt
     2341 tctccttgta gtagcgctgg acgataggaa gcgtgagcgc cgccacctcg agctcgggcg
     2401 cgtggaaggg cgttcccgga ggggtgtcgc catagagcga ttcccgcagc gcgttcaggc
     2461 tgacgccgcc ggccgcgctc agagcgccgc gggactcgcc gcgcgccttg gccaccgact
     2521 gctccagcgt gtactggttg aaggaaggat gcaggacggc gtcgctcagg atgccgagcg
     2581 cccggttcac gtaccgcgcg ggcaccgtca tgttgtagat gatgctggag tatcccgatt
     2641 cgcttcccag cgtgccgccg aaggactccg cttccttgcg gacctcgccg cgctgcttgg
     2701 tggaggtctc ctcgtacggg aggtaggaga gcgccgacgc ggcgccccgc tcgctccggg
     2761 tctcgtcgcg gcttcccgcg tcgatccaga cctggaccgt ggcgagcgga cgcgagcggt
     2821 tctcccggac gatgaccgtc atcttgttgg gaagggtgcg gatcaggttg ggtgcggcgg
     2881 cggcgggacc ggcgggtccc aggacggcgg ccgcgatcag ggcggcagcc gcggcggtca
     2941 gggcaatggg agcgaagcgc attcgggtcg aatcctccgg cgagatggta tggggtaccc
     3001 cgaaaactct actccattcg cgccgaaaaa cgaaccccgg cgggagcgcc gccggggtcc
     3061 ggaagcatcg tcatgtcgcg gcggggacgt tcccgccggg gttctagtgc tgcacgcgga
     3121 ccacgttggt ggcctgcttg cctcggttct gatcctccat cgggtcgaac tcgacgacct
     3181 ccccttcgtt caacgtgcgg aagccgtcgg cgacgatcgc ggaatgatgg acgaagaact
     3241 cttctcccga atcctcctga atgaacccgt agcccttgga ctcgctgaac catttgaccg
     3301 agccgcggtg tctcaagatc ggggcccctc ctttcggcgt gaaagcgatc gggatgaggg
     3361 tatgtcgtgg gcgaaagctc gctcgtctag acgttcatgc ctcgcgtgga ccggacgttc
     3421 tggaaacagt ccctgcagta taccggcttt cccggcgtgg gacggaacgg aacctgggtc
     3481 atctggccgc aggacgagca gacggcctcg tggaactccc gaggagccgc gttcgagatc
     3541 ggcgcggccg aaaccgggcc gccggaggcg cgcttcttct cgtcccggca gcggcggcac
     3601 cgcgtgggct cgtgctgaaa accgcggtcc ttgtagaaat tctgctcccc aaccgtgaag
     3661 acgaactctt caccacagtc gcgacatttg attaccttgt ccacgtcagc catgaagcct
     3721 ccgagacgca ctcacttctg gatgaaacaa aaacgccccc accgggtgag taccgtgggg
     3781 gcctcctgcg ggaaccgtgg gcttttggcg tacggaagca tgacgggagt tggggagatg
     3841 actgcattgt cactcgtagc tgccgatact ccgccgagca cggaccgact gagccttgcc
     3901 aacatctaac tatagggaga ggcgcgactt gtgtcaagcc atttgaggcg tttttccatc
     3961 gccggaactg cgtatcgctg cgcgattttc gtgttcgcgc tggccgccgc ggccctgatc
     4021 ggcccggccc ggccgggggc ggccgctccg gaacctcccc cccgcccctc cgcggactcg
     4081 ctcgtcgtgc tccactccaa cgacgtgcac tcccaccttc tccccttcca ccggagcgaa
     4141 gccaccctgg tcggcggagc ggccgcgcgg gcggcgctga tcgaacgcga gcgggaacgc
     4201 gacccggacc tgctcctcct ggacgcgggc gacatcgtgc aggggacgcc cgcctataac
     4261 ctgttccgcg gggtccccga cacccgcgcg atgtccgcga tgcggtacga cgccgtcgcc
     4321 ctgggcaatc acgacctcga cgacggcccc gcggcgtggc tcgcgctgcg cccgctcgcc
     4381 gagtatccgg tcctgagcgc gaacgtgttc gccgctgcgg acagcagctg ggcatcgggg
     4441 ctcggcgacg caccttccgc cgaggtccgg cgcggcgcgc gctggatcgg gggcgcgtcc
     4501 gtgtccagct cggcatccct gcggttcctg gccaagccct atctcatccg gtcgtggcac
     4561 gggaagcgga tcgcgatcct gggcctcacc acgggaagcc tcgtgaacat cgtgcgcatc
     4621 agcgccaacc ggggcgtcgc cgtctcggat ccggtccttg tcgcgcggtg gttcgtgccc
     4681 atccttcgcc aacaggcgga ctacgtgatc gccctcacgc acctcggtgt cgacgccgac
     4741 caggatctcg cgcgccgcgt ccccggcatc gatctcatcg tgggggggca cagccatacg
     4801 cgcctcgacg agccggtgtt cgccgggaac ggcacggctc ccggcctcgc gggcgtgccg
     4861 atcgtgcaga ccggggcgtg gggcgaccgc atgggccgaa gtgtcctcta cctcgcgaac
     4921 ggccgcccct ccgggttctc ctacgagctg ttgcgggtgc gcccccagga tggcgaggat
     4981 cccgcgatcg cggcccttct ccggccggtg gccgactccg tgtccgtggc gatgggggcc
     5041 accgtgtacg aatcgaccgg acgggtgccg accccccagc gcgacgacgt ggagacggcg
     5101 atcgggaatt tcgcggccga ggtcctccgc gagaccgcgg gcgcggaggt cggcatcatg
     5161 aacacgggcg ggatccgcgc tccgattccg cgcggaccgg tcaccgtggc ggacgtctat
     5221 tctacgttcc ccttcgacaa cacgatcgtc gtcgtgccga tgcgcggcga tgacctccgc
     5281 cggctcctgg acttcgcggc gcggcggatc ggcaagagcg acttcgccca ggtgagcggc
     5341 gtgcggttct cgatcagcgg cgactacgcc tcggacatcc gggtgaacgg ccatccgctg
     5401 gacgaggacc gcatctaccg ggtcgccacg atcgacttcc tctacgaggg cggcgccggc
     5461 tacacccagt tccggaaagc gggccccgcc gagcccaccg gtgttttcca gaacgaggcc
     5521 gcggtctctt tcctgcggcg ccatccccag tatcagttcc gcaccgacgg gcgcatcgcg
     5581 tgggagggcg cagcccgcgg gctgcgctcc ctgcagacgc gctgaagacg ccaagcgata
     5641 cggccctggc cggacgcccg ggcccggaat cgaaactttc ggtttgacag ccccggaggg
     5701 gcggggctac ggtccctcgc gtccgggctc ttggcgcggc ccggcaggac aacgctttga
     5761 aatcctgcat cagtgttgcc tcgcactcgt catccggctt cagcgttggc acgcggcacg
     5821 attcaaagca cgacccggtg ctcgaacgct gaagcgaaac ccgaaggacg caccgaagac
     5881 gacgaagctg gccgacggtc cggagccgtc gcgggatacg actgtccccc gcgacggctc
     5941 cgggcatttc ggcacaaggg gcgctcccgc gtccctcgga gggaggtgaa cgctcgatgc
     6001 gcaagagggg aacggtgaag tggttcaacg aggccaaggg attcggattc atccagcagg
     6061 ccggcggtga ggacgtcttc gtccactact cggcgatcca gggtgagggc ttcaagacgc
     6121 tgtccgaggg acaggtggtg gagttcgaga tcgtggaggg gccgaaggga aagcaagccg
     6181 ccaacgtgac tccggtctga cccggacgct ccttgtaacc tgaccgcccc gacgcgggta
     6241 gcgccggggc gtgtttttat aggcccggcg ggtgtaggat tcgccgctta tatatgcccg
     6301 agcaacccca ccaggaagaa ccggacccgg gacaccagat ccccgcgctg ccgcccgacc
     6361 ccccggccgg cgtgatcgtc gacccggcgc cgatccgttt cgccaagcgc ctcttcgcgg
     6421 ccgtcatcgg gccggcgcgc gcctttaccg atcccgcgct gttccacaag atctcgctcg
     6481 ccgccttcct cgcgtgggtc ggactgggcg cggacggcct ctcgtcttcg gcgtacggcc
     6541 ccgaggcggc gtggctcgcc ctggagggga acgtcttcct ggcgatcccg ctggcgctgc
     6601 tcaccgcgtt caccgtcttc gtgatcgcgg ccagctacca gcggatcatc gagcgcttcc
     6661 ccgccggcgg cggcggttac gtggtcgcga ccaagctgct cggaccggga gcggggctcg
     6721 cgtccggaag cgccctcctc gtggattacg tgctcacggt cgcgatctcg ctggcgagct
     6781 gcagcgagca ggtgttcagc gtgctcccga cgcaatacca gttcgtgaag cttcccttca
     6841 cgttcctcct gctcggggtg ctgacgctcc tgaatctgcg cggcgtgcgc gagtcagtcg
     6901 ccatcatctc gccgatcttc cttctgttca tcgtgacgca cgcggcgggt ctcgtgacgg
     6961 gcatcgtgtc gaactggggc caggtgccgc tcgagtggac ccatcttcac gagcagtctt
     7021 cggtggccgc ccagaacatc gggctggttg gcctcgtgat gatcctcttc cgcgccttca
     7081 cgctgggcgg cggcacctat acggggatcg aggcggtctc gaacggcgtg ccgatgctgc
     7141 gcgagcccaa ggtggagacc gccaagatca cgatgcgcta catggccatt tcgctggccc
     7201 tgatcgcggg cggcattctg ttctgctacc tgctctacca ggtccgcccg gttccgggac
     7261 ggacgctcaa cgcggtcctc ttctccgcga tcgcgggaac ggtatttcct cccgggagcg
     7321 gcctggggca cgcgctcgtc gcgatcacgc tcatctcttc cgccgccctc ctgttcgtgg
     7381 cggcccagac cggctttctc gggggcccgc gcgtgcttgt ctacatggcg ttcgaccggt
     7441 gggtgccgtc ccgcttctcc agcctctccg agcggctggt cacgtcgaac ggcgtcctgt
     7501 tcatggcgat ctccgcggcc atcgtcctgg ccgccaccca gggcgcggtg cacatcctgg
     7561 tcgtcctcta ctcgatcaac gtgttcctca ccttctcgct cgcccagctc ggcatgtgcc
     7621 gcgacgcgct gaacctcaag cgggagggac aaccgtggaa gcgggccttc ttcatcagtt
     7681 cgctcggctt cgtcgtgacc gggctcctcc tgatcgggac cgtctacttc aagttcaccg
     7741 agggcggctg ggccacgctc ctcgtgacgg cgatcatcgc catggtctgc gtcatgatcc
     7801 ggggccatta cgtgcggacc aaccagagcc tgcaccggct ggatcaggcg ctcacgaccg
     7861 tgccgctccc cgcgagcgag ccgacgcagg cgccgctcaa gaagaacgcg aacaccgcgg
     7921 tgctcaccgt gacctcgttc ggcgggctgg gaatccacac gctcctgaac ctgttccggg
     7981 tcttccccgg gcagttcaag caggtgatct tcatctcggt cggcgcgatc gactccggcc
     8041 agttcaaggg ggccgaagag atcgaggcgc tccgcgccag caccgaggcg gagctccgga
     8101 agtacgtgga gttcgcgcgg aagctgggga ttcccgccga gagccgcctg gcgctcggca
     8161 ccgacatcgt ggacgagctg gagcagctgt gcatcctggt tcaggagcag ttcccgcgca
     8221 gcgtcacctt cgccggccag ctggtgttcc agaaggaagg gagcatcgtc cgctggctcc
     8281 acaaccagac ctgcgtcgcg ctccagcgcc gcctcgcgtt ccacgggttg ccgatggtga
     8341 tcctgccggt gcgcgtgtat tgatgtcccg cggccggagg cctcggaagc cggcgtgagc
     8401 cgctcgtgga aggagcgcgc cgcggcggtt cgcctcgaag ggcacgccct gctgctcgcc
     8461 gcgcgcgatc cgcggacgcc gcgcttggcg agggccctcg tctggagcct cgccgcctac
     8521 gccctgtcac cgatcgatct catccccgac ttcatcccga tcctgggaca tctcgacgac
     8581 gtggtgatcg tgggggccgg tttgtggatc gcgcggcgca tgattcccga ggccgtgttg
     8641 tcggaatgcc gcgagcgggc gcggagcaag gcggcggagg gcaaggaccc gggaaaggat
     8701 cgcggcgggg cccaggggtg atggctggtg gaaggtggga ccggacccgc gccgcgtgct
     8761 gcccccgcgc ggactccctc ggcgcaaggc ggctgggcaa accctt
//
LOCUS       DASSLZ010000243        16887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1483763, whole genome shotgun sequence.
ACCESSION   DASSLZ010000243 DASSLZ010000000
VERSION     DASSLZ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 16887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16887
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483763"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VFM00_09125"
     CDS             <1..914
                     /locus_tag="VFM00_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7498450.1"
                     /translation="MQRIFRLIAKVAPTESTVLITGESGTGKEMVATAIHLQSRRAHR
                     PFIAVNAAAVPESLFESELFGHVRGAFTGAVGDRPGLLRQADGGTLFLDEVAEMPLAV
                     QAKLLRALQSGEVRPVGGAEATHVDVRVIAATNRDLPRALAEGRLREDLYYRLSVFTI
                     EMPALRERREDIPLLATFFRQRYARRIGKRVDRFTDRAQYYLMRYDYPGNVRELENAI
                     ERAVTLSEHGEITHMDLPPTFREAPVRLLREGDAFPYRADMSLADLEAEHIRRALVHF
                     AGNTTKAARSLGVSRSTLWRKMKQYGL"
     gene            1117..1542
                     /locus_tag="VFM00_09130"
     CDS             1117..1542
                     /locus_tag="VFM00_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7498451.1"
                     /translation="MSVTRWNPVRDEMTRFVDEFLGRRFESEGPTVWQPRVDIEESDE
                     TYVVRAELPGMKLEDIKITVEDNQLTIRGEKRREEEKKGTTYHRVERVYGMFERSFTL
                     GHLVNSDRIEAFYRDGVLQVSIPKAEQAKAREIAIKVEK"
     gene            1649..3592
                     /gene="dnaK"
                     /locus_tag="VFM00_09135"
     CDS             1649..3592
                     /gene="dnaK"
                     /locus_tag="VFM00_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET7498452.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGEPVVIPNAEGARTTPSVVAVTK
                     TGERLVGQVAKRQAVTNPKNTVYSIKRFMGRKYSEVVEEIKNVPYEVKEGPGGQVQIV
                     LGDKSYTPPEISAMILQKMKQTAEDYLGEKVTEAVVTVPAYFNDAQRQATKDAGRIAG
                     LDVKRIINEPTAAALAYGLDKKKDEKIAVYDLGGGTFDISILELGDGVFEVKSTNGDT
                     HLGGDDFDKRVIDWLAQEFQKQEGIDLRKDAMALQRLKEAAEKAKIELSTVMQTDVNL
                     PFITADANGPKHLNVTLTRAKYEQLVDDLIQRTKPPVERALSDAGLSPSQIDEVILVG
                     GATRTPKVQELVQKHFGKEPHKGVNPDEVVAIGAAIQGGVLAGDVKDVLLLDVTPLSL
                     GIETLGGVMTKLIERNTTIPTKKSEIFSTAADSQTTVEIHVLQGERDMAQDNKTIGRF
                     QLTGIPPAPRGIPQIEVTFDIDANGILHVTARDKATGKEQKIRIEAGGGLTEQEIKKM
                     VTDAESHAAEDKQKREQIETKNRGDALAYETEKNLKELSEKMDGADRAKVENALADLK
                     RALEAGHSEEIRTATDTLNRVWGEVSQRMYAGAAQGPGGPGEGPGPEGQGPVGGEPQG
                     GPQQGGKKEGDDGAVDADFEVVK"
     gene            3687..4190
                     /gene="msrA"
                     /locus_tag="VFM00_09140"
     CDS             3687..4190
                     /gene="msrA"
                     /locus_tag="VFM00_09140"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET7498453.1"
                     /translation="MNATVKEKATFAAGCFWGVESMFRAVPGVLDVAVGYTGGHTENP
                     TYKEVCTGRTGHAEAVEVIYDPARVTYDTLLGVFFENHDPTTPNRQGPDVGEQYRSAI
                     FVHSPEQEREAQAKVAQLEGAKRFRRPIVTQIVPETPFWRAEEYHQRYFEKKGIAPTC
                     HMPAPRP"
     gene            4333..5778
                     /locus_tag="VFM00_09145"
     CDS             4333..5778
                     /locus_tag="VFM00_09145"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7498454.1"
                     /translation="MNLNVAVIMPPGAEREALLAYLERRHAAVVLADTVDQFLATRVE
                     EACSLGVLDFGSRAQAESERSYPVDRRDAIRRLKERWPRMQLVLWLDREDAADAADMN
                     ASGIRNIFLKPIRTEALDGLLQAAGRSVVQHAREQRETARVEQEFRFERIVGESPAIR
                     RAVELAQSVSLSSATSVLILGESGVGKELFARAIHGESPRAHGPFMEINCAAIPRELL
                     ESELFGHEKGAFTDATQQRIGLFEAAEHGSVFLDEVGELPLTLQAKLLKFLDMKVVRR
                     VGGSRDIPVDVRILAATNRALSDEVRSGRFREDLYYRLNVVPIEVPPLRERNGDIVLL
                     GRLFTERVSRKLGRPVRLNAAGEKALLHYPWPGNVRELMNAVERAVLLSRAEEMGPAE
                     LALPDLQPRPIGDADLGSLGIRIPAEGISLLALEKAAIEGALEKSGGNVVEAARLLHI
                     GRGSLRCKMRRHGIGRDREEEKAAPRVGQLA"
     gene            5997..6599
                     /locus_tag="VFM00_09150"
     CDS             5997..6599
                     /locus_tag="VFM00_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7498455.1"
                     /translation="MNPTATDEELVRKSQEDDERAFGELVTRYESKVYSLAMKMLRNP
                     EDAEDVLQETFLRAYRGIKSFKGNSTFSTWIYRITANSALMRLRKKQLPQVSIEESDE
                     REAPISIADWAPGPVEQLLNQEMQKVMDEAIDALPAEFRQVFILRDVEEMSNADVAEI
                     LDLSVAAVKSRLHRARLKVRNRLAQYFSETKADRTRAKAS"
     gene            6596..6922
                     /locus_tag="VFM00_09155"
     CDS             6596..6922
                     /locus_tag="VFM00_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7498456.1"
                     /translation="MMTCREVLDSISSFIDEDLKQEVLHEMRKHIHVCSDCRAQFDSM
                     TMTIKLYRHEPKPSVPAECHDRLVKVLELERMKPPSPQDVADASGTPSPQSPPKSASG
                     TPETKS"
     gene            6942..7907
                     /locus_tag="VFM00_09160"
     CDS             6942..7907
                     /locus_tag="VFM00_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7498457.1"
                     /translation="MNSDAPRRIHLYRPQNQLGDLLLNVPAIRAIRDRYPEAKLTLIV
                     GPQNAPAVLGQLWADEVRVLRGRGGWAALREALRVAPRPDLSIYFTTVSYSVSGALLA
                     ARSGARQRIGFAPARYGRRDWAGLTRAVPYPEGKRHQSEVSMALAHAAGATVAPPPPH
                     YVPDPRLLSRVPSGVAYLHPGAGKIKNRWPVERFAEVAAGLARRGLEVRVLEGPQDSG
                     TAAALSARVGRELLVVRGETVPMLAARFARAAVYVGNDTGPLHLAGAVGCPTVGVYGW
                     TDPDEWKPVGARVRAARAADAKLESVTAAMVLEAVTAALEEGVRV"
     gene            7904..9874
                     /locus_tag="VFM00_09165"
     CDS             7904..9874
                     /locus_tag="VFM00_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET7498458.1"
                     /translation="MREATRRAWHGTAVAAILLVPVFAVAAPPAGPARSAAPSARPAS
                     LNAAALSAREAHRDTTGLAHVLLRGSSPSLRASAARALGRIQNRGSVTALSAALRDPV
                     APVRREAAFALGLVGDSTAAPALAARLGVEADAEARVSMLTALGYVGARRGGPALATA
                     LRSARAVERHAAALAAGRARDSSLVEPLALAAKGEDADTRWRAAYALGRIGDRRAGPA
                     LEPLLADPNPLVRSMAARAAGDVADSAAAPRLVALLADPAWRVRVNAAHALGALKAPA
                     GASGLRAALQDSSPHVRWESALSLGALRDTAAAAPLRVALADSATGVVQGAAIALLQI
                     QGEAAVPTVAPALDLLPPFLRSGLIDALGDLAGPASLEILLARVRDDSDPAEAAGAAS
                     ALSRRAADRRAAIPALRAALRSKDFTVVASAAEGLGTLGDSTSVPALAQLLVARAKPD
                     DADVAASAATALAALKTSDALAALRSARNDPERRIRETATRALGLPDDSVAATPAPPL
                     RVDPLPARSATRATVVTERGTVLIELRPHEAPRTVENFARLARSGYFNGLEFHRVVPN
                     FVIQDGCPRGDGWGGPGYSIPCEYNDLPYGIGAVGMALAGKDTGGSQWFITLSPQPRL
                     EGRYTVFGEVTKGMEVVERIMPGDRIVKIEVK"
     gene            10021..10587
                     /locus_tag="VFM00_09170"
     CDS             10021..10587
                     /locus_tag="VFM00_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498459.1"
                     /translation="MGNRRELSQQLLLTIALLLAAFGTAQAKSFESALDPPRWAVKSL
                     LASGIEVWPGDLGGAAYTVSRHLSPNYVEGDFDGDGKSDIAILIRRKADDKFGMAILL
                     RAQAKSGTKATILGAGSPFGTGGNDFRRMDAWSVQSQESFPPKIRSAWKGPQPKTKRD
                     GLVLQKRGSTEALVTYDGAKFVWTPYGG"
     gene            complement(10594..11478)
                     /locus_tag="VFM00_09175"
     CDS             complement(10594..11478)
                     /locus_tag="VFM00_09175"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET7498460.1"
                     /translation="MSPLPRLKPKPAEPEGPEPFDAEVAIRVIRKRTPITPRTGLVLG
                     SGLGVVADGVAWDAAFPTTDIPGLPRPTVAGHPGRILIGRWAGRPVAVAQGRSHLYEG
                     HSAEETTRTVRLFAALGIKSLVLTNAAGGIATRMTPGTLMLVEDQVNLQWRSPMRVPV
                     EREPPLELPRGPRGLSSRTGYSEELMAKAARAAVVAGVRLERGVLGVVLGPSYETAAE
                     IAMLERMGADAVCMSTGAEAAIAAGLGIPAVAISCVTNWATGRSRARLSHAEVTAAVS
                     AAAAPMKSMMERLILALA"
     gene            complement(11546..12433)
                     /locus_tag="VFM00_09180"
     CDS             complement(11546..12433)
                     /locus_tag="VFM00_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498461.1"
                     /translation="MTLDWTALLRFPLEWAALALFVASCLYWERAGASGLGVEGCVAS
                     AMIGLILGYEYSESWGVALAAGVGSAALFAVVAGLLLHLLRSDPAVGSFCLSLVPAAA
                     LGLLTRAGPYRLLEVKPLPGIVHGTVLGGTPSEDLLMNPILWAAPLVVALAAWVLWQT
                     PFGLRLRAFGEAPSLRLPGARSGLYRLTGLVLGALWAVPGAALLLGAHRPSPPVGVGF
                     LALACVVAARWSFAFAILLALGPALLRAAQPAVAGTPLAVPVEIAPYVLALVYLVFLS
                     RRALRMPETRRTRVDPDVL"
     gene            complement(12430..13065)
                     /gene="udk"
                     /locus_tag="VFM00_09185"
     CDS             complement(12430..13065)
                     /gene="udk"
                     /locus_tag="VFM00_09185"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET7498462.1"
                     /translation="MTDGRRGILIGIAGGTGAGKTLVAQTIAEDLGNDEVLILEQDMY
                     YKDLQAIPLGERENRNFDHPDAFDSHLLRAQLETLLAGGSVDIPIYDMRTHTRLAQGR
                     RAAGRRVIILDGLLILEDPSIRQLMDIKLYVDADPDIRFIRRMKRDLTERGRTLDSII
                     RQYESSVRPMHLQFVEPSKRYADLIVPEGGYNFVAIDLLKTKIRALLSETR"
     gene            complement(13066..13620)
                     /gene="hpt"
                     /locus_tag="VFM00_09190"
     CDS             complement(13066..13620)
                     /gene="hpt"
                     /locus_tag="VFM00_09190"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET7498463.1"
                     /translation="MEVLISAADIQRRVCEVAKEIARDHPDTSPLLIGVLNGCVMFVA
                     DLMKAIPIPHEIDFVSVSSYRQASESNRSPRLLKDIEKEILGRDVVLVEDIVDSGHTL
                     AFLRETLLQRGPRKLRIATLLDKPGRRERQVPLDYVGFTVPDRFVVGYGLDYAERYRH
                     LPYVAALTAAEIETDPSRRQVGSR"
     gene            13756..14142
                     /locus_tag="VFM00_09195"
     CDS             13756..14142
                     /locus_tag="VFM00_09195"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HET7498464.1"
                     /translation="MERPKTERRGLQRRRRDRRAEGRMERQLFWMLWGVAFMNYLDAG
                     QTVYLLNVKLMVEANRLMAWILDHSPYAFWLYKTLVPSLGCFLLWKFRKRVRWMYGAV
                     LTVFAVYFTVVLRSFLYMLLPIGPPV"
     gene            14423..15229
                     /locus_tag="VFM00_09200"
     CDS             14423..15229
                     /locus_tag="VFM00_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498465.1"
                     /translation="MGGTYGGSYLRLAIDPAARGPVAREDVGYFFINSFLLSRIDSDL
                     PSDLAEFDEDVNIYTAALLAGVVSGREDLLRADLVSTRDADVFARVQDSKDQRLRYQV
                     YKANADFLYVSLGVFTEAVAADSGTVEPDAVRLTGRGKSYYQFASAYAERLYGSRAGI
                     AEVMDKLSRNFEAYTRVMSWMSGEYLNLVRRLSEGEVFHIERDAQVRFEDAHKSHTWD
                     DLLDSYVEWQKTHAIEARRRLMRAARELKRVDPDFSLRAFRDKWGSPRRS"
     gene            15318..16628
                     /locus_tag="VFM00_09205"
     CDS             15318..16628
                     /locus_tag="VFM00_09205"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET7498466.1"
                     /translation="MSARIRIGLLGCGVVGAGFLRLLASRSRAVESAAGAPLRVTRLA
                     VRDPNRRREVDLSGVEVGTDPLAVASADDVDLLVELMGGAETARPAVERALERGIPVV
                     TANKHLISVHGAALEAAAAASGAPLLYEASVAGVIPVLQALDHGLRTERFTLLVGILN
                     GTTNYILSTMEREGRSFEEALAAARELGYAEADPTLDLSGADTAQKLTVLARRAFGAP
                     VPHDSIPTEGIVGLELEDLRQADRFGYVVKLLGIGIRSAEGLDLRVHPAFIPKRYLLA
                     SVRDEFNGVYLRGDASGSMLLYGKGAGALPTAQAVLGDVIAVARELLLRGGAPAGARR
                     PARAASQAPEDERLPLDEVRTKYYLRLMVEDQPGVLAQIAHRLGASRVSVAQMHQAQS
                     VRGQASITMLTHETRDKDAREAVAAIASIPSVLKGPRAVRIFDL"
     gene            16667..>16887
                     /locus_tag="VFM00_09210"
     CDS             16667..>16887
                     /locus_tag="VFM00_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7498467.1"
                     /translation="MARPWRGIVREFRDILPPIPEEAVVTLLEGNTPLVPAPRLAARI
                     APGLELYVKVEGQNPTGSFKDRGMTVAVSR"
BASE COUNT         2707 a         5829 c         5906 g         2445 t
ORIGIN      
        1 ggatgcagcg catcttccgg ctcatcgcca aggtcgcgcc caccgagagc accgtgctca
       61 tcaccggcga gagcggcacc ggaaaggaga tggtcgcaac cgcgatccac ctccagagcc
      121 gccgcgccca ccgtcccttc atcgccgtga acgccgcggc ggtccccgag tccctgttcg
      181 agagcgagct gttcgggcac gtgcgcgggg ccttcaccgg cgcggtgggg gaccgcccgg
      241 ggctgttgcg ccaggcggac ggcggcacgc tctttctcga cgaagtggcc gagatgccgc
      301 tcgccgtgca agccaagctg ctccgcgcgc tccagagcgg ggaggtgcgg ccggtcggcg
      361 gcgccgaggc gacgcacgtc gacgtgcgcg tcatcgccgc cacgaatcgc gacctgcctc
      421 gcgccctcgc cgaggggcgc ctgcgcgaag acctctacta ccgcctcagc gtgttcacga
      481 tcgagatgcc ggcgctccgg gagcgccgcg aggacatccc gctcctggcc acgttcttcc
      541 gccagcgcta cgcccgccgg atcggaaagc gcgtggaccg cttcaccgac cgcgcccagt
      601 actacctgat gcgctacgac tacccgggca acgtgcgcga gttggagaac gccatcgagc
      661 gcgcggtgac gctctccgag cacggcgaga tcacgcacat ggacctgcct cccacgttcc
      721 gggaggcgcc ggtccggctg cttcgcgagg gcgacgcgtt cccctaccgg gccgacatgt
      781 cgctcgcgga tctggaggcc gagcacatcc ggcgggccct ggtccacttc gccgggaaca
      841 ccaccaaggc agcccgctcg ctcggcgttt cccggtcgac gctctggcgg aagatgaagc
      901 agtatggttt atagttgctc tataatggat caataagcgt tcgataatgg aacgtaacgg
      961 tggcgggtcg gcgcacccct ttccattaaa tggcgtattt gtagggactt acgataactg
     1021 tgggatttgg cacatcggct gcacagctct cctgtcgagc gaagccgcct gggaggcggc
     1081 gagttcaaca aaacaagaat gaggaggaga gccaaaatgt cagtgacccg ttggaatccc
     1141 gttcgtgatg agatgacccg cttcgtcgac gagttcctcg gccgccgctt cgagtcggag
     1201 ggaccgaccg tgtggcagcc ccgcgtcgac atcgaagagt cggacgagac ctacgtcgtg
     1261 cgcgccgagc tcccggggat gaagctggag gacatcaaga tcaccgtcga ggataatcaa
     1321 ctgaccatcc ggggtgagaa gcgccgggag gaagagaaga agggcaccac ctatcaccgc
     1381 gtggagcgcg tgtacggcat gttcgagcgc tcgttcacgc tgggccacct ggtgaacagc
     1441 gaccggatcg aggccttcta ccgcgacggc gtgctccagg tgagcattcc caaggccgag
     1501 caggccaagg cgcgggagat cgcgatcaag gtcgagaagt agcgagcagc atccaaacca
     1561 tcgaacgttg cggcgggcgg ggtccggcca aagccgggcc cgcccgtatt cgcaggagcc
     1621 gtgaagcggc cccgaaaccg gagggatcat gggaaaaatc ataggaatcg acctcggcac
     1681 gacgaactcg tgtgtcgccg tgatggaagg cggtgagccg gtcgtcattc cgaatgccga
     1741 gggagcccgg acgaccccct cggtcgtggc tgtcaccaag accggcgagc gcctcgtcgg
     1801 tcaggtcgcc aagcggcagg cggtcaccaa ccccaagaac accgtctatt cgatcaagcg
     1861 attcatgggg cggaagtact ccgaggtggt cgaggagatc aagaacgtcc cctacgaggt
     1921 gaaggaagga ccgggcggcc aggtgcagat cgtcctgggc gacaagtcgt acacgccccc
     1981 cgagatctcc gcgatgattc tccagaagat gaagcagacc gcggaagact atctcggcga
     2041 gaaggtgacg gaggcggtcg tcacggtgcc cgcgtacttc aacgacgcgc agcgccaggc
     2101 caccaaggac gccgggcgga tcgcgggtct cgacgtgaag cgcatcatca acgagccgac
     2161 ggcggccgcg ctggcgtacg gcctcgacaa gaagaaggac gagaagatcg ccgtctacga
     2221 cctgggcggc ggcacgttcg acatttccat cctcgagctg ggcgacggcg tgttcgaggt
     2281 caagtccacg aacggggaca cccacctggg cggcgacgac ttcgacaagc gcgtgatcga
     2341 ctggctggcg caggagttcc agaagcagga agggatcgat ctccggaagg acgccatggc
     2401 gctccagcgg ctgaaggaag ccgcggagaa ggccaagatc gagctctcca cggtgatgca
     2461 gaccgacgtg aacttgccct tcatcacggc ggacgcgaac ggtcccaagc acctgaacgt
     2521 cacgctgacg cgggccaagt acgagcagct ggtggacgac ctgatccaac ggaccaagcc
     2581 gccggtggag cgcgcgctct ccgacgcggg gctctctccg tcgcagatcg acgaggtgat
     2641 cctggtcggc ggcgccacgc gtacgcccaa ggttcaggaa ctggtccaga agcatttcgg
     2701 caaggagccg cacaagggcg tgaacccgga cgaggtcgtc gcgatcggcg ccgcgatcca
     2761 gggcggcgtg ctcgccggcg acgtgaagga cgtgctgctg ctcgacgtga ccccgctctc
     2821 cctggggatc gagaccctgg gcggcgtcat gaccaagctg atcgagcgga acaccacgat
     2881 cccgaccaag aagtcggaga tcttctcgac cgcggcggac agccagacca ccgtcgagat
     2941 ccacgtgctc cagggcgagc gcgacatggc ccaggacaac aagacgatcg ggcgcttcca
     3001 gctcaccggg atcccgcccg cgccgcgcgg cattccgcag atcgaggtga cgttcgatat
     3061 cgacgccaac ggcatcctgc acgtcacggc ccgcgacaag gcgaccggga aggagcagaa
     3121 gatccggatc gaggccggcg gcggtttgac cgagcaggag atcaagaaga tggtgaccga
     3181 cgccgagtcg cacgcggcgg aggacaagca gaagcgcgag cagatcgaga ccaagaaccg
     3241 cggcgacgcg ctcgcgtacg agaccgagaa aaatctcaag gagctttcgg agaagatgga
     3301 cggcgcggat cgagcgaagg tcgagaacgc gctcgccgat ctgaagcgag cgctcgaggc
     3361 gggtcactcg gaggagatca ggaccgccac ggacacgctg aaccgggtct ggggagaggt
     3421 ctcgcagcgg atgtacgccg gcgcggcgca ggggccggga ggcccgggcg aaggtccggg
     3481 gccggaaggc cagggcccgg tcggcggcga gccgcagggc ggaccgcagc agggcggcaa
     3541 gaaggaaggc gacgacggcg cggtggacgc ggacttcgag gtcgtgaagt agcggcggcg
     3601 tcgcagccca gcccgtcgta cgtactgatg gcgccccggg aggaagtccc ttccggggcg
     3661 ttcgttcccc aacccgagga gtttccatga acgcgacggt gaaggagaag gccacgttcg
     3721 ccgccggctg cttctggggc gtggagtcga tgttccgcgc cgtgccgggc gtgctggacg
     3781 tcgccgtggg ctacacgggc ggtcacaccg agaaccccac ctacaaggag gtctgcacgg
     3841 gccgcacggg ccacgccgag gcggtggagg tgatctacga ccccgcgcgg gtgacctacg
     3901 acacgctgct cggcgtcttc ttcgaaaacc acgacccgac cacgccgaac cgccagggtc
     3961 ccgacgtggg cgagcagtac cgctcggcga tcttcgtcca ttcgccggag caggagcggg
     4021 aagcccaggc caaggtggcc cagctggaag gcgcgaagcg cttccgccgg ccgatcgtca
     4081 cccagatcgt ccccgagacg cccttctggc gcgcggagga gtaccaccag cgctatttcg
     4141 agaagaaggg gatcgctccg acatgccaca tgccggcgcc gcggccctga ggtcccgggg
     4201 ccgcgccccg ggccgactcc cggcctcgcg tcagccgacc cgaatccgct aagcatttcc
     4261 gtccccctgc cgataactgt tccgtcacgg gctcagtgcg tttgcagcgg cccgatgggg
     4321 gaatccgact ccttgaatct gaacgtcgcc gtcatcatgc cgccgggagc cgagcgggag
     4381 gcgctgctcg cctacctgga gcggcgccat gccgcggtcg tcctcgccga cacggtggac
     4441 cagttcctgg ccacgcgcgt ggaagaggcg tgctcgctgg gcgtgctcga cttcgggtcg
     4501 cgcgcgcagg cggagagcga gcggagctac cccgtggacc ggcgggacgc catccgacgg
     4561 ctcaaggagc gctggccgcg catgcagctc gtgctctggc tggaccgcga ggacgccgcc
     4621 gacgccgccg acatgaacgc gagcggcatc cggaacatct tcctgaagcc gatccgcacc
     4681 gaggcgctgg acggcctgct ccaggcggcc ggccggagcg tggtgcagca cgcccgcgag
     4741 cagcgggaga ccgcgcgcgt cgagcaggaa ttccggttcg agcggatcgt gggggagagc
     4801 cccgccatcc ggcgcgcggt cgagctggcg cagagcgtct cgttgagcag cgcgacgtcc
     4861 gtgctgatcc tgggggagag cggcgtgggc aaggagctct tcgcccgggc gatccacggc
     4921 gagagccccc gcgcacacgg ccccttcatg gagatcaact gcgccgcgat cccgcgcgag
     4981 ctcttggaga gcgagctgtt cggccacgag aagggcgcgt tcacggacgc gacccagcag
     5041 cggatcggtc tcttcgaggc ggccgagcac ggctcggtct ttctcgacga agtcggcgag
     5101 ctgccgctta cgctgcaggc caagctcctc aagttcctcg acatgaaagt cgtgcggcgc
     5161 gtgggcggct cgcgcgacat cccggtggac gtgcgcatcc tggcggcgac caaccgcgcg
     5221 ctgtcggacg aggtgcgctc cgggcgcttc cgcgaggacc tctactaccg cctcaacgtc
     5281 gtgccgatcg aggtgccgcc gctgcgcgag cggaacggcg acatcgtcct gctcgggcgc
     5341 ctcttcaccg agcgcgtctc gcggaagctg gggcgcccgg tgcggctgaa cgcggcgggc
     5401 gagaaggcgc tgctccacta tccgtggccc ggcaacgtcc gcgagctgat gaacgcggtc
     5461 gagcgcgccg tgctcctgag ccgcgccgag gagatgggcc ccgcggagct cgcgctgccc
     5521 gatctccagc cgcgaccgat cggcgacgcc gatctcgggt cgctcggcat ccggatcccc
     5581 gccgagggga tctcgctcct cgcgctggag aaggcggcca tcgagggcgc cctcgagaag
     5641 agcgggggca acgtggtcga ggcggcccgg ctcctccaca tcggccgggg cagcctgcga
     5701 tgcaagatgc gccggcatgg catcgggcgc gaccgcgagg aagaaaaggc cgccccccgg
     5761 gtcggccagc tggcctgatc cggcctccgg gccgccccgt ttcgaaacac ccctccgtcg
     5821 aggattcttc gccgcgcccg ttccgtcgcg aaccccgctc ccaggccccg catcctaagt
     5881 cgctgtgccc ctgcgtctgg aacggccctc cttgacaccc cccggtttgc tccgtagcct
     5941 cgatggaggt tttcggaacc tgttgtccgg taccggcatc catgactgag gagttcgtga
     6001 atccaacagc gaccgacgag gaactcgtcc gcaaatcgca ggaggatgat gagcgcgcct
     6061 tcggcgagct cgtcacgcgg tacgagtcca aggtctacag cctggcgatg aaaatgctgc
     6121 ggaatccgga ggacgcggaa gacgtccttc aggaaacgtt cctccgggcg tatcgcggca
     6181 tcaaatcgtt caaggggaac tcgacgttct ccacgtggat ctaccgcatc accgcgaact
     6241 cggcgctgat gcggcttcgg aagaagcagc ttccccaggt ctcgatcgag gaatcggacg
     6301 agcgggaagc gccgatctcc atcgccgact gggcgcctgg ccccgtcgag cagctgttga
     6361 accaggaaat gcagaaagtc atggacgagg cgatcgatgc gctcccagcc gaatttcgac
     6421 aagtcttcat cctgcgggac gtggaagaga tgtcgaatgc ggacgtggcg gagatcctcg
     6481 atctctcggt ggcggccgtg aaatcacgcc tccaccgggc acgcctcaag gtccgtaacc
     6541 gtctcgcaca gtacttcagc gagaccaaag ccgatcggac gagggccaaa gcatcatgat
     6601 gacgtgccgc gaggtgctgg attcgatttc atcgttcatc gacgaggacc tgaagcagga
     6661 agttcttcac gaaatgcgga agcacatcca cgtgtgcagc gattgccgcg cccagttcga
     6721 cagcatgacg atgacgatca agctctaccg ccacgagccc aagcccagcg ttcccgccga
     6781 gtgccacgac cggctggtga aggtcctcga gctcgagcgg atgaagccgc cgagtcctca
     6841 ggacgtcgcg gacgcctcgg gcactccaag tccccagagt ccgcccaaga gcgcgtccgg
     6901 cacccccgaa acgaagagct gacgggcccg ccaccgggtc catgaattcc gacgccccgc
     6961 gtcgaatcca tctctaccgc cctcagaacc agctcggcga tctcctcctc aacgtcccgg
     7021 ccatccgggc catccgcgac cggtaccccg aagccaagct gacgctgatc gtcgggcctc
     7081 agaacgcgcc cgccgtcctg gggcagctct gggccgacga ggtgcgcgtg ctgcgcggcc
     7141 gcggcgggtg ggccgccctt cgcgaagccc tccgcgtcgc gccgcgcccc gatctctcga
     7201 tctacttcac caccgtttcc tattccgtga gcggcgccct gctcgcggcg cggtccgggg
     7261 cgcgccagcg gatcgggttc gcgccggcgc ggtacggccg ccgcgactgg gcgggtttga
     7321 cgcgggcggt accctatccc gaagggaagc ggcatcagtc ggaggtctcg atggcgctgg
     7381 cccacgccgc cggggccacg gtcgcgccgc ctccgccgca ctacgtcccc gatccccgcc
     7441 tgttgtcgcg tgttccctcc ggggtggcgt atcttcaccc cggcgccggc aagatcaaga
     7501 accgatggcc cgtggaacgc ttcgcggagg tggcggcggg gctcgcgcgc cgcggtctgg
     7561 aggtgcgcgt gctcgaaggg ccgcaggatt cgggaacggc ggcggcgttg agcgcacgcg
     7621 tcggtcgcga gctactggtc gtgcgcggcg agaccgtgcc gatgctggcc gcccgcttcg
     7681 cgcgcgcggc ggtctacgtg gggaacgaca cgggaccgct tcatctcgcc ggggcggtgg
     7741 gatgtcccac cgtgggagtg tatggctgga ccgatcccga cgagtggaag cccgtgggcg
     7801 cgcgggtacg cgcggcgcgc gcggccgatg cgaaactgga gtcggtgacg gcggcgatgg
     7861 tcctggaagc cgtgacggcg gcgctggagg agggggtgcg ggtttgaggg aagcgacgag
     7921 acgcgcgtgg catgggaccg cggtggccgc gatcctcctc gtgccggttt tcgccgtggc
     7981 agcgccgccg gcgggccccg cccgctctgc cgcaccgtcc gcgcgcccgg cgtccctgaa
     8041 cgccgcggcc ctctccgcgc gcgaggccca tcgggatacg acggggctcg cccacgtcct
     8101 cctccgcggc tcctcgcctt cgctgcgcgc ctcggccgct cgagccctgg ggcggatcca
     8161 gaaccgcggg tcggtgacgg cgctctccgc cgcgctgcgc gatccggtcg cgcccgtacg
     8221 ccgtgaagcg gcgttcgcgc tcggcctggt cggcgactcg acggccgcgc cggccctggc
     8281 ggcccgcctg ggcgtcgagg ccgatgccga ggcgcgggtc tcgatgctca ccgcgctcgg
     8341 ctacgtcggc gcgcgccgcg gcggccccgc gctggcgacc gccttgcgct ccgcgcgggc
     8401 ggtcgaacgg cacgccgcgg cgcttgccgc cggccgcgcg cgggactcct cgctggtcga
     8461 gccgctcgcc ctcgccgcca agggcgagga tgccgacact cgctggcgcg cggcctatgc
     8521 cctgggccgg atcggggacc ggcgggccgg ccccgcgctg gagccgctgc tcgccgatcc
     8581 gaacccgctc gtccgctcca tggccgcccg cgcggcgggg gacgtcgcgg actcggcggc
     8641 cgcgccgcgc ctggtcgcgc tcctcgccga tccggcatgg cgcgtccgcg tgaacgcggc
     8701 ccacgcgttg ggagcgctga aggcgccggc gggcgcgtcc gggctccgcg cggcgctcca
     8761 ggattcgagc ccccacgtgc ggtgggaatc ggcgctctcc ctgggcgccc tgcgcgacac
     8821 cgcggccgcg gcgccgcttc gggtggcgct cgccgacagc gcgaccggcg tcgtccaggg
     8881 agccgcgatc gcgctgctcc agatccaggg cgaggccgcc gttcccacgg tcgcgcccgc
     8941 cctcgacctg ctgcctccgt tcctgcgatc gggactcatc gacgcgcttg gcgatctcgc
     9001 cggccccgct tcgctcgaga tcctgctcgc gcgcgtgcgc gacgattccg atcccgcgga
     9061 ggcggcgggc gcggcgtcgg cgctctcgcg ccgcgcggcc gatcgccgcg ccgcgatccc
     9121 cgcccttcgc gcggcgctca ggtcgaagga tttcaccgtc gtcgcctccg cggcggaggg
     9181 gctgggcacg ctcggcgatt ccacttcggt gcccgcgctg gcgcagctcc tcgtcgctcg
     9241 cgccaagccc gacgacgccg acgtggccgc ctccgccgcg acggcgctcg cggcgctcaa
     9301 gacttcggat gcgctcgccg cgttgcgctc cgcgcgaaac gaccccgagc ggcgtatccg
     9361 cgagaccgcg acgcgagcgc tggggcttcc cgacgactcg gtcgcggcca cgcccgcgcc
     9421 tccgctccgc gtggatccgc ttccggcgag gtccgccacg cgggccaccg tcgtgaccga
     9481 gcgggggacg gtgctgatcg agcttcgccc ccacgaggcg ccgcgcaccg tcgagaactt
     9541 cgcgcggctc gcgcgctcgg gatacttcaa cgggctcgag ttccatcgtg tcgtaccgaa
     9601 cttcgtcatc caggacggct gcccgcgcgg cgacggctgg ggcggccccg gatattcgat
     9661 tccgtgcgag tacaacgacc ttccctacgg catcggcgcg gtcggcatgg cgctcgcggg
     9721 caaggacacc ggcggcagcc agtggttcat cacgctgtcc ccgcagcccc ggctggaagg
     9781 gcgctacacc gtgttcggcg aggtgacgaa ggggatggag gtggtggagc ggatcatgcc
     9841 gggggatcgg atcgtgaaga tcgaagtgaa gtgagcgaca ccacgatggg atgaaccggc
     9901 catttggcga tcacgaaagg ctgctctcgc gccgatgaat gagagggtcc ttgtcgcgga
     9961 tcaacatgct ccagagcatg ttgattgtgc cgttttgact cgcatgaatg aggtgcggtg
    10021 atgggaaacc gtcgtgagct gagccagcag cttcttctga cgatcgccct gcttctcgcc
    10081 gcattcggga ccgcgcaggc caagagcttc gagtcggcgc tcgatccgcc gcggtgggcc
    10141 gtgaagtccc tgctcgcgag cggcatcgag gtctggcccg gcgatctggg aggcgccgcc
    10201 tacaccgtgt cgcgacatct cagccccaac tacgtcgagg gcgacttcga cggcgacggg
    10261 aagagcgaca tcgccatcct cattcggaga aaagccgacg acaagttcgg catggcgatc
    10321 ctcctccgcg cgcaggccaa atcgggcacg aaagccacca tcctcggggc aggctccccg
    10381 ttcgggaccg gagggaacga cttccgccgg atggacgcgt ggtcggtcca gagccaggag
    10441 agcttcccac cgaagatccg gagcgcctgg aaagggccgc agccgaaaac caagcgcgac
    10501 ggcctggtcc tccagaagcg gggatcgacg gaagcgctgg tgacctacga cggcgccaag
    10561 ttcgtgtgga ctccatacgg cggctgagac ggatcaggcc agcgccagga ttaaccgctc
    10621 catcatgctc ttcatcggcg cggcggccgc gctcaccgcg gcggtgacct ccgcatgcga
    10681 caaccgcgcg cggctccgtc ccgtggccca gttggtgacg caggaaatcg cgaccgcggg
    10741 aatgccgagc ccggcggcga tggccgcctc ggcgcccgtg gacatgcaca ccgcgtcggc
    10801 gcccatccgc tcgagcatgg cgatctccgc cgccgtttca tacgaagggc cgagcaccac
    10861 gccgagaacg ccgcgctcca gccgcactcc cgcgaccacg gccgcgcgag cggccttcgc
    10921 catcagctcc tcggagtagc ccgtccgcga cgacaacccg cgcggaccgc gaggcagctc
    10981 cagcggcggc tcccgctcca ccggcacccg catcggcgag cgccactgca gattcacctg
    11041 atcctcgacc agcatcagcg tgccgggcgt cattcgcgtg gcgatgccgc ccgccgcgtt
    11101 cgtgagcacg agcgacttga tcccgagcgc cgcgaagagc cgtacggtgc gcgtcgtctc
    11161 ctcggcgctg tgtccctcgt acaggtgcga gcgtccctgc gccacggcga ccgggcgtcc
    11221 cgcccagcgg ccgatcagga tccggcccgg gtggccggcc acggtggggc gcggaagtcc
    11281 cgggatgtcg gtcgtgggga acgcggcgtc ccatgccacg ccgtcggcca cgacgcccag
    11341 gccgcttccc agcacgagtc cggtgcgcgg cgtgatcggc gtgcgcttcc ggatgacgcg
    11401 gatggcgacc tcggcgtcga acggctcggg gccttcgggc tcggcgggct tgggcttgag
    11461 gcgggggagc gggctcatcg gggcacgggt ctcacgacga aggccaagcg aaccgcacga
    11521 cgcgcggcgg ccggcacaac gaccctcaca gcacgtccgg gtccacgcgc gtccggcgcg
    11581 tctccggcat ccggagcgcg cggcgcgaca ggaagacgag atagaccagc gcgagcacgt
    11641 agggcgcgat ctcgaccggc acggccagcg gcgttccagc gacggcgggc tgcgcggcgc
    11701 gcagcagcgc gggtcccagc gcgagcagga tcgcgaacgc gaacgaccac cgcgcggcca
    11761 cgacgcacgc cagcgccagg aagccgacgc ccaccggcgg gctcgggcga tgggccccca
    11821 ggagcagcgc cgcgccgggc acggcccaga gcgcgcccag cacgagcccg gtcagccgat
    11881 acaaccccga ccgcgcgccc ggaagccgga gcgagggcgc ctcgccgaaa gcgcggaggc
    11941 ggagcccgaa gggcgtctgc cagaggaccc aggccgccaa ggcgaccacg agcggtgccg
    12001 cccagaggat cggattcatg aggagatcct ccgagggggt cccgccgagc accgtgccgt
    12061 gcacgattcc cgggagcggc ttcacctcga ggagccggta gggaccggcg cgcgtcagga
    12121 ggcccagcgc cgcggcgggc acgaggctga ggcagaagga gccgaccgcc gggtcggagc
    12181 gaagcaggtg gagcagcagt cccgccacga ccgcgaagag ggccgccgat cccacgcccg
    12241 ccgccagcgc gacgccccag ctttcgctgt actcgtagcc caggatcagc ccgatcatgg
    12301 ccgaggccac gcacccctcg acgcccaggc ccgaagcgcc cgcgcgctcc cagtagagac
    12361 aggaggccac gaagagcgcg agtgccgccc attcgagggg aaagcggagg agcgccgtcc
    12421 agtccaacgt catcgcgttt cgctgagcag cgcgcggatc ttcgtcttca ggaggtcgat
    12481 cgcgacgaag ttgtagccac cctcgggaac gatgaggtcg gcgtagcgct tcgagggctc
    12541 gacgaactgc aggtgcatcg ggcggacgct cgattcgtac tggcggatga tggagtcgag
    12601 cgtgcggccg cgctcggtca ggtcgcgctt catgcgccgg atgaaacgga tgtcgggatc
    12661 ggcgtcgacg tacagcttga tgtccatgag ctggcggatc gagggatcct ccaggatcag
    12721 gaggccatcc aggatgatca cgcggcgccc ggcggcgcgg cgcccctgcg cgaggcgcgt
    12781 gtgcgtgcgc atgtcgtaga tcgggatgtc gaccgagccc cccgcgagga gcgtctcgag
    12841 ctgcgcgcgg aggaggtggc tgtcgaaggc gtcggggtgg tcgaagttac ggttctcgcg
    12901 ctcgcccagc gggatcgcct ggaggtcctt gtagtacatg tcctgctcga ggatcaggac
    12961 ctcgtcgttg cccaagtcct cggcgatcgt ctgggcgacg agggtcttgc cggcgcccgt
    13021 gccgccggcg atgccgatga ggatcccgcg gcgcccgtcg gtcacctagc ggctgcccac
    13081 ctgtcgcctg gaggggtccg tttcgatctc ggccgcggtg agcgcggcga cgtaggggag
    13141 atggcggtag cgctccgcgt agtcgagccc gtagccgacg acgaaccgat cgggcacggt
    13201 gaatccgacg taatccagcg ggacctggcg ctcgcggcgc cccggtttgt cgagcagggt
    13261 ggcgatgcgc agcttgcgcg gtccgcgctg gagcagggtc tcgcgcagga aggccagggt
    13321 gtgcccgctg tccacgatgt cctcgaccag cacgacgtcc ctgcccagga tctccttctc
    13381 gatgtccttc aggagcctcg gggagcggtt ggactcggac gcctgccgat aggacgagac
    13441 gctcacgaag tcgatctcgt gcgggatggg gatcgctttc atcagatcgg cgacgaacat
    13501 gacgcagccg ttcaggacgc cgatgagcag cgggctggtg tcgggatgat cgcgcgcgat
    13561 ctccttggcc acctcgcaga ctcgtcgctg gatgtccgcc gcgctgatca acacttccat
    13621 ggggtgggcc tccggtgcgg tttcgggcgc actctagcat cgcccccggg gggtgtcaaa
    13681 tcgggcgcaa gttccgactc ccgttgccga taatgggcgg aagcggggag tcgtccaggg
    13741 aggaaaggga gggtcatgga gcgaccgaag acggagcgcc gcggcctgca gcgccggcgc
    13801 cgggaccgcc gggccgaagg gcgcatggag cggcagctct tctggatgct ctggggcgtc
    13861 gccttcatga actacctgga cgccgggcag accgtctacc tccttaacgt gaagctgatg
    13921 gtcgaggcca accggctgat ggcctggatc ctggaccact ctccctacgc cttctggctc
    13981 tacaagacgc tggttccctc gctgggctgc ttcctcctct ggaaattccg caagcgcgtg
    14041 cgctggatgt atggcgcggt gctcaccgtc ttcgcggtct atttcacggt cgtgctccgc
    14101 agctttctct acatgctgct gccgatcgga ccccccgtct aagcggcaag aaacctcccg
    14161 ccgcgcccga tcgggcataa cgcaacgcca cccaggtcct gaggcctctt gccacagctg
    14221 aggcccccgc gtccccgcgg aaccccctcc cccctggctg ttgataagtg gctaagtatt
    14281 ataataccag aaacttaact aacaaccgcc gcccgccgcg cattgtaccg attcgtgcat
    14341 tttccaatgg catgttcggt gcatgggcgt atttcccggt agcccgccgt ggtgccgagg
    14401 gcgggtggaa cggaggaagc aaatgggcgg aacgtacggc ggttcgtacc tgcgtctggc
    14461 catcgatccg gcggcgcgcg gcccggtggc gcgtgaagac gtcggctatt ttttcatcaa
    14521 ctcctttctc ctttcgcgca tcgattccga tcttccttcc gacctcgccg agttcgacga
    14581 ggacgtgaac atctatacgg cggcgctcct cgccggcgtc gtgtcgggcc gtgaggacct
    14641 ccttcgcgcg gacctggttt caactcggga tgcggatgtc ttcgctcgcg tgcaggacag
    14701 caaggaccag cggctccggt atcaggtgta caaggcgaac gccgacttcc tgtacgtgtc
    14761 gctcggcgtg ttcaccgagg cggtcgcggc ggattcgggt acggttgagc ccgacgccgt
    14821 ccgcctgacg gggcgtggca aatcctacta tcagttcgcg tcggcgtacg ccgagcgcct
    14881 ctacggctcg cgcgcgggca tcgccgaggt gatggacaag ctctcgcgca acttcgaggc
    14941 ctatacccgc gtgatgtcgt ggatgagcgg ggagtacctg aacctggtcc gccggctgag
    15001 cgagggcgaa gtcttccaca tcgagcgcga cgcgcaggtc cgcttcgagg acgcccacaa
    15061 gagccacacg tgggatgacc tcctggattc gtacgtggaa tggcagaaga cgcacgcgat
    15121 cgaagcgcgc cgccgcctca tgcgcgccgc gcgcgagctg aagcgcgtgg atcccgactt
    15181 ctcgctccgc gccttccgcg acaagtgggg ctcgccgcgc cgctcgtagc cccaggcgcc
    15241 gcgccgctcc cagcaatcgc ccccgaccgc gctcccgttt gacagccccg gagcggtcca
    15301 gtaggttggg ccgcctcatg tcggcgcgca tccgcatcgg tctcttaggc tgcggcgtgg
    15361 tcggcgcggg gttcctgcgt ctgctcgcct cgcgctcgcg cgcggtggag tcggccgccg
    15421 gcgcgccgct ccgcgtgacg cggctggctg tacgcgatcc taaccggcgg cgcgaggtgg
    15481 acctctccgg tgtggaggtc ggcaccgacc ccctcgccgt cgcttccgcc gacgacgtgg
    15541 acctgctcgt cgagctgatg ggcggggccg agacggctcg ccccgccgtg gagcgggcgc
    15601 tggagcgcgg catccccgtc gtcaccgcga acaagcacct catctccgtt cacggcgcgg
    15661 cgctcgaggc ggccgcggcc gcatccggcg ccccgctcct ctacgaagcc tccgtcgcgg
    15721 gcgtcatccc ggtcctccag gcgctggacc acgggcttcg caccgagcgc ttcacgctcc
    15781 tcgtcggaat cctgaacggc accacgaact acatcctctc gacgatggag cgcgaagggc
    15841 gctcgttcga agaggcgctc gccgccgcgc gcgagctggg atacgccgag gcggatccga
    15901 cgctcgatct ctccggcgcc gacaccgccc agaagctcac ggtcctcgcg cggcgcgcgt
    15961 tcggcgcgcc ggtgccgcac gactccatcc ccaccgaggg gatcgtcggc ctcgagctgg
    16021 aagacctgcg ccaggcggac cgcttcggtt acgtcgtgaa gctcctgggc atcggcatcc
    16081 gctcggccga ggggctcgac ctgcgcgtgc accccgcctt cattcccaag cgctacctgc
    16141 tggcctcggt gcgcgacgag ttcaacgggg tctatctgag gggggacgcc tcgggctcga
    16201 tgctcctcta cgggaaaggg gccggcgcgc tccccacggc gcaggcggtg ctgggcgacg
    16261 tgatcgcggt cgcgcgcgag ctcctcctcc gtggcggcgc acccgcggga gcgcggcgcc
    16321 cggctcgcgc cgcctcccag gcgcccgagg acgagcggct gccgctggac gaggtgcgga
    16381 ccaagtacta cctgcgcctg atggtcgagg atcagcccgg cgtcctggcg cagatcgccc
    16441 accggctcgg cgcctctcgc gtgagcgtgg cccagatgca ccaggcgcaa tccgtgcgcg
    16501 ggcaggcctc gatcaccatg ctcacgcacg agacgcggga caaggacgcg cgcgaggcgg
    16561 tggcggcgat cgcctcgatc ccgtccgtcc tcaagggccc gcgcgccgtc cggatcttcg
    16621 acctgtgagc cggggcgccg cttccaccgg ccccgcgggc ccagccgtgg cgcgtccgtg
    16681 gcgcgggatc gtgcgcgagt tccgcgacat cctgccgccg atccccgagg aggccgtggt
    16741 cacgctcctc gaggggaaca cgccgctcgt gcccgcgccg cgcctggccg cgcgcatcgc
    16801 tccggggctc gagctgtacg tcaaggtgga ggggcagaat cccaccggct cgttcaagga
    16861 ccgcggcatg accgtcgccg tctcgcg
//
LOCUS       DASSLZ010000244         6325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1610679, whole genome shotgun sequence.
ACCESSION   DASSLZ010000244 DASSLZ010000000
VERSION     DASSLZ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610679"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="VFM00_09215"
     CDS             <1..1887
                     /locus_tag="VFM00_09215"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET7498468.1"
                     /translation="ASGTVSLIRTRRSHAMLLAARAQGGPVVLVNGFDARVTTPRGTF
                     DLASHRYVPDVVHPDGHRRIESFTTDPWPTWVFHLEDGTRIEQQLFTVSGAAGVILTW
                     SVVDGTAAGVQLMVRPLISGREARALHHENPHFRAEAVCCDTSVEWRPYDSVPAILAG
                     SNGHYGHHPFWYKNFLYEADRAAGRDHVEDLHSPGAFRFDLGRGEAVLVLTTAEGLRA
                     MEAHAAARAAGLPGFVGPTSGDPEGALEALRESERAARADAPTPLLRAAESYVVRSGS
                     AAGVAAGYPRTRASSRETFASVRGLCIATGRLDDAKRILLPWVPTLAQGLFPTTFPEG
                     AAQPRYDSVDAPLWFIIAAHELLCASAEGRRRVSAAERNTLRGAIEAILPWYVEGTKH
                     RIRMDEDGLLAAGEPDARVTWMDKACGAIAPRIGKAVEVQALWLNALRAGAEISNRWE
                     ALYHRGRGSFERRFWNEGAASLHDVVDLNHERGVADASFRPNQILAIGGLPWPVFEGK
                     RARQVVDLVEARLFTPMGLRAGPAVDPAAPRLAWPWMLGPFVEAWVRVRDNMPEARAE
                     ARERFLAPLLARLGEGGVGQGGLGHLPEAVDADPPQAERGAPFYALSVAEAIRLDQVV
                     LPRL"
     gene            1905..3647
                     /locus_tag="VFM00_09220"
     CDS             1905..3647
                     /locus_tag="VFM00_09220"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HET7498469.1"
                     /translation="MSTSGGSPPLATPPPRHYPWQRTALALAVATQGVINLFSALLSH
                     PPERLLALRHLVPTDVMDQSRTFTFVAGALLIATAWGLRRGKRRAFVVALFLAALSVP
                     MNLLKAIDFEEATAASVLLFALGISGDAFRVKSRELTFGAIGQRTLWTGAFFLVYAVA
                     GCYWLEAHYGGGASLGRAVSEAAYRLFGIGEPHLETIAPLHTQASRMARWFTHSLAPI
                     GALILVGTALALLRPVAHRERHRTERERVARLLRHYGESSVGAFALEPDVDYFFSPTG
                     RAVIAYRFESNVLLVLGDPIGPDDEIPPLLQQFATYCAEHDWTFALFQARPEKLAIYR
                     EAGWRAVHIGVDPMLRLDRFTLEGSAMGDVRRSRNKLEAAGLEARLYPPDSHPFDPSR
                     ESAMRDQLAAVSSEWLRSRKGAEKGFAMGRFDTNRLADQWLAAAYNPERQRVEAFITL
                     VPIWARRGWALDLMRRRDDAPSGVIEFLIVHCAEAAKERGDELISLSLSALAKTEADA
                     ADAGDRARAFLMEHLRRFYDFEGLFRWKSKFAPDFEPRYLVYADPLALPRVVFALARA
                     QSPGGFRSYFRKAA"
     gene            4375..4935
                     /locus_tag="VFM00_09225"
     CDS             4375..4935
                     /locus_tag="VFM00_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7498470.1"
                     /translation="MKRSLITSSLAALSVAFLLPMGAHAATTTTTNATAAKHTTAAKS
                     ATAAKTTTAAKSASTTPATNATASKPTRRHHTMAKNASATKAKATKIDINTATREELM
                     TLPGIDGDEADKIIAGRPYKSMAQLKTKGGLTNAEYRKISRKVTAKQAAAMHSEAPAN
                     GTPSTDSGAQSSSGSSASGSSDQTTK"
     gene            5135..5770
                     /locus_tag="VFM00_09230"
     CDS             5135..5770
                     /locus_tag="VFM00_09230"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HET7498471.1"
                     /translation="MSPTIRHALWCAALMAGIGLAAPASATDKAPAKDAGKPAAGQPS
                     QQEMMAAMMKMAAPGPEHAALNPLAGSWKTVTKMWNSPSGEPVTSEGTCERSWIMGGR
                     YLVANYKGDFAGMAFEGMEVLGYDNMKKQYVSSWVDNMGTGIMMTQGPSMDPATHSYT
                     LSGTMIGPSGQETTAREVTNIVDGNTYTMTMFASDHGQEQKMMEITYSRKK"
     gene            5784..>6325
                     /locus_tag="VFM00_09235"
     CDS             5784..>6325
                     /locus_tag="VFM00_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7498472.1"
                     /translation="MNSEATTTGAPRSGSRSRAPLVLAIAITLVTWSSAYAAIRHAVR
                     GFPPASLALIRFAIASVTLALLTVPTRRVSLRGISRRDLAGFALMGFVAIAIYHTALN
                     AGERTVHAGAASLLINTGPIWTALIAMVFLGERLGTRGWIGTALAFLGAVIISVGAAG
                     GIRFEGDVGFILIASLCQAV"
BASE COUNT          934 a         2318 c         2160 g          913 t
ORIGIN      
        1 gcctcgggca cggtttccct catccgcacc cgccggtcgc acgcgatgct gctcgcggcg
       61 cgcgcgcagg gcggccccgt ggtcctggtc aacggtttcg acgcgcgcgt caccacgccg
      121 cgcggcacgt tcgacctggc ctcgcaccgc tacgtgcccg acgtcgtcca tcccgacgga
      181 catcgccgca tcgaatcgtt caccaccgac ccctggccca cctgggtctt tcacctggag
      241 gacggcaccc gcatcgagca gcagctcttc accgtgagcg gcgccgcggg agtcatcctc
      301 acctggagcg tcgtggacgg caccgcggcc ggcgtgcagc tgatggtccg gccgctgatc
      361 tccgggcgcg aggcgcgcgc gctccaccac gaaaacccgc acttccgggc cgaggcggtc
      421 tgctgcgaca cctcggtcga gtggcgcccc tacgacagcg tccccgcgat cctcgccgga
      481 tccaacggtc actacggaca ccaccccttc tggtacaaga acttcctcta cgaggccgac
      541 cgcgccgccg gccgcgacca cgtggaggac ctgcactccc ccggcgcgtt ccgcttcgac
      601 ctgggccgcg gcgaagcggt gctcgtgctg acgaccgccg aggggcttcg cgccatggag
      661 gcgcacgccg ccgcgcgggc ggcgggactg ccgggattcg tcgggcccac gagcggcgat
      721 cccgagggcg cgctggaggc gctgcgcgag agcgagcgcg ccgcccgcgc cgacgccccc
      781 acgccgctcc tgcgcgcggc cgagtcgtac gtggtccgga gcgggagcgc ggcgggcgtc
      841 gcggcgggct atccgcgaac gcgcgcctcc agccgcgaga cgttcgcttc ggtccgcggg
      901 ctctgcatcg cgacgggacg cctcgacgat gcgaagcgga tcctgctccc ctgggtgccg
      961 acgctggccc agggactgtt ccccaccacg ttccccgagg gcgccgccca gccccggtac
     1021 gactcggtgg acgcgcccct ctggttcatc atcgccgcgc acgagctcct gtgcgcctcg
     1081 gccgagggcc ggcgccgcgt ctcggcggcg gagcgcaaca cgctccgcgg cgcgatcgag
     1141 gcgatcctcc cctggtacgt cgagggaacg aagcaccgga tccgcatgga cgaggacggg
     1201 ctgttggccg cgggcgagcc ggacgcgcgc gtgacgtgga tggacaaggc gtgcggggcg
     1261 atcgcgccgc gcatcgggaa agcggtcgag gtgcaggcgc tctggctgaa cgcgctccgc
     1321 gcgggggcgg agatctcgaa ccggtgggag gcgctctacc atcgcgggcg cggctcgttc
     1381 gagcggcgct tctggaacga gggcgccgct tcgctccacg acgtcgtgga cctgaaccac
     1441 gagcgcggcg tggccgacgc ttcgttccgc cccaaccaga tcctcgcgat cgggggcctt
     1501 ccatggcccg tgttcgaggg gaagcgcgcg cgccaggtgg tggacctggt cgaggcgcgc
     1561 ctcttcacgc cgatggggct tcgcgcggga cccgccgtcg acccggctgc gccccgtctg
     1621 gcctggccct ggatgctggg gcccttcgtc gaggcgtggg tgcgcgtgcg cgacaacatg
     1681 cccgaggccc gcgccgaggc gcgcgagcgc tttctcgcgc cgcttctggc gcggctgggc
     1741 gagggcggcg tcggccaggg cgggctgggc cacctgcccg aagccgtgga cgccgatccg
     1801 ccgcaggccg agcggggggc acccttctac gcgctctccg tcgccgaggc gattcgcctg
     1861 gaccaggtgg tgctgcctcg tttgtgaaga cgcttccgat ggacatgagc acttccggcg
     1921 gttcgccgcc gctcgccacg ccgccgccgc gccactatcc ctggcagcgg accgccctcg
     1981 cgctcgccgt cgcgacgcag ggcgtgatca acctgttctc ggcgctgctc tcgcacccgc
     2041 ccgagcgcct cctcgcgctt cgacatttgg tcccgaccga cgtcatggac cagagccgca
     2101 cgttcacgtt cgtggcgggc gcgctcctga tcgcgacggc ctggggtctg cgccgcggga
     2161 agcgccgcgc gttcgtggtc gcgctcttcc tggccgcgct ctccgtgccg atgaacctgc
     2221 tcaaggcgat cgacttcgag gaagcgaccg ccgcctcggt gctcctcttc gctctgggga
     2281 tcagcggcga cgccttccgc gtgaagagcc gcgagctgac cttcggcgcg atcggccagc
     2341 gcacgctctg gaccggcgcc ttcttcctgg tctacgcggt ggccggctgc tactggctgg
     2401 aggcccacta cggaggcggc gcctcgctgg gccgcgcggt gtcggaggcc gcctaccgcc
     2461 tcttcggcat cggcgaaccc cacttggaga cgatcgcgcc gctgcacacc caggcctcgc
     2521 ggatggcccg gtggttcacc cattcgctcg cgccgatcgg cgccctgatc ctcgtgggca
     2581 cggcgctggc gctcctgcgt ccggtcgcgc accgcgagcg ccaccgcacg gagcgggagc
     2641 gggtggcgcg gctgttgcgc cactacggcg aatcctcggt cggcgccttc gcgctcgagc
     2701 ccgacgtgga ctacttcttc agcccgaccg gccgcgccgt gatcgcctac cgcttcgagt
     2761 cgaacgtgct cctggtgctg ggcgatccca tcggcccgga cgacgagatc ccgcccctgc
     2821 tccagcagtt cgcgacctat tgcgccgagc acgactggac cttcgcgctc ttccaggcgc
     2881 gccccgagaa actggcgatc taccgcgaag cgggatggcg cgcggtgcac atcggcgtgg
     2941 accccatgct ccggctggac cgcttcacgc tggaggggtc ggcgatgggc gacgtgcggc
     3001 gctcgcggaa caagctggag gcggcggggc tcgaggcgcg gctctatccg cccgactcgc
     3061 acccgttcga cccctcgcgc gaatcggcga tgcgcgacca gctggcagcc gtgtcgtcgg
     3121 agtggctccg ttcgcgcaag ggggcggaaa agggattcgc gatgggccgt ttcgacacga
     3181 accgtctcgc cgaccagtgg ctggcggcgg cctacaaccc ggagcgccag cgagtggagg
     3241 cgttcatcac gctcgtgccg atctgggcgc ggcgcggctg ggcgctggac ctgatgcgcc
     3301 gccgcgacga cgccccctcc ggcgtgatcg agttcctcat cgtgcactgc gccgaggcgg
     3361 ccaaggagcg cggggacgag ctgatctccc tgtccctctc ggcgctggcc aagaccgagg
     3421 cggacgccgc cgacgccggc gaccgggcgc gcgcgttcct gatggagcac ctccggcgtt
     3481 tctacgactt cgaggggctc ttccgctgga aatccaagtt cgcgcccgac ttcgagccgc
     3541 gctacctggt ctatgccgac ccgctcgccc tcccccgggt ggtcttcgcc ctggcccggg
     3601 ctcagagccc cggcggattt cgttcctatt tccggaaggc ggcatgatag cgtcgtcgca
     3661 cgacaaattg gcagcccccg gaaacggaac cggcgccaac ggtcggggta tgcgtgcgac
     3721 agacgccgtt ctgctgttcg cggcatccaa ggagaatatg agaccgcttc gctggttcgt
     3781 catcgcgctg gtggcagggt gcctgggtct gatggcccag acggcatccg ccgccacgca
     3841 ttccgttgcg agacaccatc gctccgcgac gatgctgcgc cagaccagcc gcgccgccgc
     3901 gaaccgcacg gtgccggcgt cccgtcaggg acggcgcctt ccctcgaatc cagcgaaccg
     3961 gtcaaaaacg cgccactcga ccgcccctac cggccgcggc cgcggacatt cgacctatgg
     4021 cgttcttccc cacgcggcga acgcgacgcc ggatgaccgc tatggggcgt ttcgtcttgc
     4081 cgccgtggac gatcccggcg cgcgactgaa tcggatgctg gagagccgtg gacctccgcg
     4141 ggcgggtccc cattggatcc ccgcgtctcg cgggttctac tcccctctcc cgcgcacctc
     4201 tccagctccg tacatctcgc agtccaactc caggcagcac cgatcgttcc gctcaccccg
     4261 acaaaccgat gactcaccca ggtccggctc tttggccaag gaccccctct tgccgtccgt
     4321 tttcgagggc gcggcggcgg gacctgacat gccctcaacc gaaggagtcc cttcatgaag
     4381 cgcagcctga tcacctcatc tctggccgca ctcagcgtgg cgttcctgct gccgatgggc
     4441 gcgcacgcgg ccaccaccac cacgaccaac gcgacggcgg ccaagcacac gacggccgcg
     4501 aagtccgcga cggcggccaa gaccacgacc gcggcgaagt cggccagcac cacgccggcc
     4561 accaacgcga ccgcgagcaa gccgacccgg cgccatcaca cgatggccaa gaacgcctcg
     4621 gccacgaagg ccaaggccac caagatcgac atcaacacgg cgacgcgcga ggagctgatg
     4681 acgctccccg gcatcgacgg cgacgaggcg gacaagatca tcgccggtcg tccgtacaag
     4741 agcatggcgc agctgaagac caagggcggc ttgaccaacg ccgagtaccg gaagatcagc
     4801 cgcaaggtca ccgcgaagca ggcggcggcg atgcattcgg aggcgccggc aaacgggacg
     4861 ccgagcacgg acagcggggc ccagtcgagc agcggtagca gcgcgagcgg gtcaagcgac
     4921 cagaccacga agtaaggcga ccgttttctc catgccgtaa tccggcagcc ggtggccggc
     4981 ccccggcacc ctcggacccg cgcgtgcggt cgcaaggccg cacgtcgggt cgccgggcgg
     5041 gtccgaactc cgcccgaggg gaagccggct ccgcgttttc tgctatcctc ccgaaccacc
     5101 cccaagcccc accgcgaaca gggaggacgc aggaatgagc ccgacgattc gacacgcatt
     5161 gtggtgcgcc gcgctgatgg cgggcatcgg cctcgcggcg ccggcctcgg cgaccgacaa
     5221 ggcgccggcc aaggatgccg gcaagcccgc cgcgggacag ccgtcccagc aggagatgat
     5281 ggcggcgatg atgaagatgg cggctcccgg ccccgagcac gccgcgttga accccctcgc
     5341 cggttcctgg aagaccgtga ccaagatgtg gaacagcccc tcgggcgagc cggtcaccag
     5401 cgagggcacc tgcgagcgca gctggatcat gggcggccgc tacctggtgg cgaactacaa
     5461 gggcgacttc gccggcatgg ccttcgaggg gatggaagtc ctgggctacg acaacatgaa
     5521 gaagcagtac gtcagctcgt gggtcgacaa catgggcacc gggatcatga tgacccaggg
     5581 accctcgatg gatcccgcca cccactccta tacgctgagc ggcacgatga tcggcccgtc
     5641 aggccaggaa acgacggcgc gcgaggtcac gaacatcgtc gacggcaaca cctacacgat
     5701 gacgatgttc gcgagcgacc acgggcagga acagaagatg atggagatca cctacagccg
     5761 taagaagtga gccgcggctc gcggtgaact ccgaagccac gacgacaggg gcgcctcgat
     5821 cgggatcgcg gtcgagagcg ccccttgtcc tggccatcgc gatcacgctg gtcacctggt
     5881 cctcggcgta cgccgcgatc cgccacgccg tgcgcgggtt ccctcccgcc tccctcgccc
     5941 tgatccgctt cgcgatcgcg tcggtcacgc tcgcgctcct gaccgtgccg acgaggcggg
     6001 tctcgctgcg cgggatctcc cggcgcgacc tggcgggctt cgccctcatg ggattcgtgg
     6061 cgatcgcgat ctaccacacc gcgctgaacg ccggcgagcg cacggtgcac gccggcgcgg
     6121 cgagcctgct catcaacacg ggaccgatct ggaccgccct gatcgcgatg gtgttcctgg
     6181 gcgagcggct gggcacgcgc ggctggatcg ggaccgcgct cgccttcctc ggcgcggtga
     6241 tcatctcggt gggcgcggcg ggcgggatcc gcttcgaggg ggatgtcggg ttcatcctga
     6301 tcgcctcgct ctgccaggcg gtcta
//
LOCUS       DASSLZ010000245         6508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1347709, whole genome shotgun sequence.
ACCESSION   DASSLZ010000245 DASSLZ010000000
VERSION     DASSLZ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347709"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFM00_09240"
     CDS             <1..502
                     /locus_tag="VFM00_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HET7498473.1"
                     /translation="HPEVKRAEAEVEARTQRLLSIRGGRTVTSFHRELGKIMWEYCGM
                     ARSKESLTKALGMIPPLREEFWKNAAVLGDEKELNQALEHAGRVADFMELAELLCLDA
                     LEREESCGGHFRVEHQTPDGEALRDDETFCHAAVWEYQGQDRKPLRHVEPLVFETVHL
                     AQRSYK"
     gene            499..1278
                     /locus_tag="VFM00_09245"
     CDS             499..1278
                     /locus_tag="VFM00_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET7498474.1"
                     /translation="MNLTLHVWRQKNASERGRFVTYEARDISPDMSFLEMLDVVNEGL
                     TLKGEEPVAFDHDCREGICGTCGMVINGRAHGPRARTTACQLHMRSFEDGDTITIEPW
                     RAAAFPVIRDLVVDRSPFDRIIAAGGFITAPTGSAPEANSLPVPKENAELAMDAAACI
                     GCGACVAACPNASAMLFTAAKVAHLGLLPQGQPERERRVRAMVTQMDTEGFGGCTNHR
                     ECEAVCPKGITIDNIARLNADYIRASAGYGKRMAADEGGAG"
     gene            complement(1310..4021)
                     /locus_tag="VFM00_09250"
     CDS             complement(1310..4021)
                     /locus_tag="VFM00_09250"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HET7498475.1"
                     /translation="MAPYVPSHEPHKIKTVRLISFPTLEERKRRLAEARFNVYRLPPA
                     HVTFDMLSTGTSARSQEQLAGELLGDEAYAGARNFEALTAAVNQVLGHTYVCPAHNLL
                     GCVKLVATALLPQGGTVASNARGRAGVYAARQVRLVDVRDAREPVFTGNVDLPALRAA
                     LAEEEVSLIDLQAFADGMHPFSLANLEAVRAAADKRGVRLVLDASRVIENAWYIQRHE
                     PGQDTRKIADIVKQISKTAHIVAIDGAQDPMGPIGGVLATDNPADHERFQNEVVVYEG
                     LHTYGGMSGRAMESMARGLLEMGDEAEAQWAMRETEHFTERLRTAGVPLERGCDGAYL
                     EAARFLPAHRQYAAHALAAGLYLSSGVRAVVPGPSTEGDYLPVQCPRLAMTTWQLDQV
                     ADAIVNVYRQRDKVARLELMTGGAWNDELEFRSVFSDLDPYEFDCYPFVVHTIERIPS
                     TTREQREGAVREAGYNTFLLRSADVTIDLLTDSGTSAMSIDQWAAYEAARATAATSDA
                     YLRFVQTIRETYGYQHVVPTHQGRAAEHILSEILIRPGQLVPGNMYFTTTKLHQELAG
                     GVFADVIVDEAHDPASTFPWKGNIDLEKLKGLVARHGAEQIAYVSFEQSVNMAGGQPV
                     SMRNMQEVYALCSELGIPVFFDATRAVENAYFIQKRDPHYHATHIRDILREMMVYGDG
                     FTISGKKDFLVNIGGVLAFKDNAEWKRKADALLRVYEGGVADGGLCPGDLAAMERGVI
                     EMLDDRYIRTRVEQAAFLGRLLTEGGVPIVTPAGSHAIFIDARRFLPHVDQDQYPAQR
                     LAAEIYIETGVRVMERGNVSKGRDPDTGKNYRPALELVRLTIPRRVYTNDHMRAVAEG
                     IIRVWKRRESIGGLAFVYEPPHLRFFRGRFAPAEEVAVPRETAASVG"
     gene            complement(4234..5004)
                     /locus_tag="VFM00_09255"
     CDS             complement(4234..5004)
                     /locus_tag="VFM00_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7498476.1"
                     /translation="MQLYPKFLKGIALAALASVLLYGAALAVPSPSYPSYHPGSDAAL
                     ASATPAATQSASDARILFRTSDRLLARGTFQRGGGSLAGNVASIGTQSSRSKTRYMLS
                     GMVLAALTPLAIHHFGEPGDKSVAVQTQTPGSSSLANLGDAISSGNGHGRGGDKGNGG
                     DKGGGGGGEDCNNDNGDNGGDNGDDDGGVVCDTGDHGDNGGSGDNGGDCDNGGDHDSG
                     GTGGDHGGGGTSLPEPGTVPLVGAGLMMALAFRSRRAR"
     gene            5345..>6508
                     /locus_tag="VFM00_09260"
     CDS             5345..>6508
                     /locus_tag="VFM00_09260"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HET7498477.1"
                     /translation="MSRWNYPVVIVGGGAAGLMAALFASEAGARVLLVERTRDGGRKI
                     VMSGGGRCNVLPSTLDPSRFVTESSPNTMRKLLLAWPLDEQRRFFETVVGIPLALEAE
                     TGKLFPVSNRSRDVRDALVALAARRGVERRFDTRVTGAAPASDGGWRLTVEDGEPIPA
                     STVVLATGGLSVPATGSDGAGLAIAQRLGHAMHDTYPALTPLVAQPHRFAALSGISLP
                     VALEAPLGPGKRFRSEGGFLFTHKGYSGPAVLNLSHLCVRARLEGREQPVFARWTPLD
                     EAAWEAELREGEGTLLAALRARLPARLAEMLLEESGVDGSIALGQLRREDRRRVVLAL
                     TRFPLFWTGDEGYGKAEVTGGGVSLAEVEPRTLESRRCPGLFLCGEMLDAFGPI"
BASE COUNT          948 a         2220 c         2321 g         1019 t
ORIGIN      
        1 gcaccccgag gtgaagcgcg ccgaggccga ggtcgaggcg cgaacccagc ggctgctctc
       61 gatccggggc ggccgcacgg tcacctcgtt ccaccgcgag ctggggaaga tcatgtggga
      121 gtactgcggc atggcgcgct ccaaggagag cctcacgaag gcgctcggga tgattccgcc
      181 gctgcgcgag gagttctgga agaacgccgc ggtgctcggc gacgaaaagg agctcaatca
      241 ggcgctggag cacgcggggc gggtggcgga cttcatggag ctggccgagc tcctctgcct
      301 ggatgctctg gagcgcgagg aatcgtgcgg cggacacttc cgcgtggagc accagacgcc
      361 ggacggcgag gcgctgcgcg acgacgagac gttctgccat gccgccgtct gggaatacca
      421 gggtcaggat cggaaaccgc tccgccacgt ggagcctctc gtcttcgaga ccgtccacct
      481 cgcccagcgg agctacaagt gaacctgacc ctccacgtct ggcgccagaa gaacgcttcc
      541 gagcgcgggc gcttcgtgac ctacgaggcg cgcgacatca gccctgacat gtccttcctc
      601 gagatgctgg acgtggtaaa cgaagggctg acgctgaagg gggaggagcc ggtcgcgttc
      661 gatcacgatt gccgcgaggg gatctgcggc acgtgcggga tggtgatcaa cggacgcgcg
      721 cacgggccga gggcccggac caccgcctgc cagctccaca tgcgctcctt cgaggacggc
      781 gacacgatca ccatcgagcc ctggcgcgcg gcagccttcc cggtgatccg ggacctcgtc
      841 gtggaccgct cgccgttcga ccgcatcatc gcggcgggcg gattcatcac cgctccgacc
      901 ggcagcgctc ccgaggcgaa cagcctgccg gtgccgaagg agaacgccga gctcgccatg
      961 gacgccgcgg cctgcatcgg ctgcggggcg tgcgtcgccg cgtgcccgaa cgcctcggcc
     1021 atgctcttca ccgcggccaa ggtggcgcac ctcgggctcc tgccgcaggg gcagcccgag
     1081 cgcgagcggc gcgtgagggc gatggtgacg cagatggaca cggaaggctt cggcgggtgc
     1141 accaatcacc gcgaatgcga ggcggtctgc cccaagggga tcacgatcga caacatcgcg
     1201 cgcctgaacg ccgactacat ccgggcctcg gcgggatacg ggaaacgcat ggctgccgac
     1261 gagggcggcg cgggctagac cgacccggcc ggggctcccg gccctccgat cagcctacgg
     1321 acgcggcggt ttccctgggc accgcgacct cctccgccgg cgcgaaccgc ccgcggaaga
     1381 accgcagatg gggcggctcg tacacgaagg ccagcccgcc gatcgattcc cgccgcttcc
     1441 agacccggat gatcccctcg gccaccgcgc gcatgtggtc gttcgtgtag acgcggcgcg
     1501 gaatcgtaag gcgaaccagc tccagagccg ggcggtagtt cttcccggta tcgggatcgc
     1561 gccccttcga cacgttgccg cgctccatca cgcgcacgcc cgtctcgatg tagatctccg
     1621 cggccagccg ctgcgccgga tactggtcct ggtccacgtg gggcaggaag cgccgcgcgt
     1681 cgatgaagat cgcgtggctt cccgccggcg tgacgatggg gacgcccccc tcggtcagca
     1741 gacgtcccag gaacgcggcc tgctccaccc gcgtgcggat gtagcggtcg tcgagcatct
     1801 cgatcacccc gcgctccatg gcggccagat cgccggggca cagcccgccg tcggcaacgc
     1861 ccccctcgta cacccggagc agcgcgtcgg ccttccgctt ccactccgcg ttgtccttga
     1921 acgcgagcac gcctccgatg ttcacgagga agtccttctt cccggagatc gtgaagccgt
     1981 cgccgtagac catcatctcg cgcaggatgt cccggatgtg cgtggcgtgg tagtgcgggt
     2041 cgcgcttctg gatgaagtag gcgttctcca cggcgcgcgt cgcgtcgaag aacacgggga
     2101 tgccgagctc gctgcagagc gcatagacct cctgcatgtt ccgcatgctg accggctggc
     2161 cgccggccat gttcacgctc tgctcgaacg agacgtaggc gatctgctcc gcgccgtgcc
     2221 gcgcgaccaa ccctttcagc ttctcgagat cgatgttccc tttccaggga aaggtcgagg
     2281 ccgggtcgtg cgcttcgtcc acgatcacgt cggcgaacac gccgcccgcc agctcctggt
     2341 gcagcttcgt ggtcgtgaag tacatgttgc cggggaccag ctgcccgggg cggatcagga
     2401 tctcgctcag gatgtgctcg gccgcgcgcc cctggtgcgt gggaacgacg tgctgatagc
     2461 cgtaggtctc gcggatcgtc tggacgaagc gcaggtaggc gtcgctggtc gccgccgtcg
     2521 cgcgcgccgc ctcgtaggcg gcccactggt cgatgctcat cgcggaggtg ccggagtcgg
     2581 tgaggaggtc gatcgtgacg tccgccgagc ggagcaggaa cgtgttgtag ccggcttcgc
     2641 gcacggcccc ctcgcgctgc tcccgcgtcg tcgaggggat ccgctcgatc gtgtgcacga
     2701 cgaacgggta gcaatcgaat tcgtagggat cgaggtcgga gaagacggag cggaactcca
     2761 gctcgtcgtt ccaggcgccg cccgtcatca gctccaggcg cgccaccttg tcgcgctggc
     2821 ggtagacgtt cacgatcgcg tcggcgacct ggtccagctg ccacgtggtc atcgcgagcc
     2881 gggggcattg gaccggcagg tagtcgccct ccgtcgaagg tccggggacc accgcgcgca
     2941 cgccggagga gagatagagc ccggccgcga gcgcgtgcgc cgcgtactgc cggtgcgcgg
     3001 ggaggaagcg cgccgcctcg agatacgcgc cgtcgcagcc acgctccagc ggcacccccg
     3061 cggtgcgcag ccgctcggtg aagtgctcgg tctcgcgcat ggcccactgc gcctcggcct
     3121 cgtcgcccat ttccaggagc ccgcgcgcca tcgattccat cgcgcgaccc gacatgccgc
     3181 cgtaggtgtg cagcccctcg tagacgacga cctcgttctg gaaacgctcg tgatccgcgg
     3241 ggttgtcggt ggcgaggacg cccccgatgg gacccatcgg atcctgcgcg ccgtcgatcg
     3301 ccacgatgtg cgccgtcttc gagatctgct tgacgatgtc ggcgatcttc cgcgtgtcct
     3361 ggcccggttc gtggcgctgg atgtaccacg cgttctcgat cacgcgcgag gcgtccagca
     3421 cgaggcgcac gccgcgcttg tcggcggcgg cgcgcacggc ctcgagattg gccaggctga
     3481 aggggtgcat tccgtcggcg aacgcctgga ggtcgatcag ggagacctcc tcctcggcca
     3541 gcgccgcgcg aagcgcgggg agatcgacgt tccccgtgaa caccggctcg cgcgcgtcgc
     3601 gcacgtccac gagccgcacc tgccgggccg cgtagactcc ggcccttcca cgggcgttgc
     3661 tggccacggt gcccccctgc ggcaacagcg ccgtggcgac cagcttcacg cacccgagga
     3721 ggttgtgggc ggggcagacg tacgtgtgtc ccaggacctg gttcacggcg gcggtcagcg
     3781 cctcgaaatt cctcgcgccc gcgtacgcct cgtcgcccag gagctcgccg gccagctgtt
     3841 cctggctccg cgcgctcgtg cccgtggaga gcatgtcgaa cgtgacgtgg gcgggaggga
     3901 gccggtagac gttgaagcgg gcctcggcca gacggcgctt ccgctcctcg agcgtgggaa
     3961 aggagatcag ccggacggtc ttgatcttgt ggggctcgtg cgacgggacg tatggcgcca
     4021 ccgatggctc ctcgcgcggg gaatgggacg atgagggtgt cccatccagg ctagcgcatg
     4081 gaggcgcgaa gggaagtggt acctcggtcc actcgggggc tggaaggagg ttggcgcgcg
     4141 ccgcctcgcg gggaggcgct attggcgcgg gaccttacgg cgcgccgcgg gggaacgaac
     4201 ccccccgcgg ccgcctccgg tcaggaagcc gtgctaacga gcccggcgcg aacgaaacgc
     4261 gagggccatc atgagtcccg cgccgacgag cggaacggtt cccggctcgg ggaggctggt
     4321 gccgccgcca ccgtggtcac cgccggtgcc gccactgtcg tggtcgccgc cgttgtcgca
     4381 gtcaccgccg ttgtcgccgc tgccgccatt gtcgccgtgg tcgccggtat cgcataccac
     4441 gcccccgtcg tcatcgccgt tgtcgccgcc attgtcgccg ttatcgttgt tgcagtcttc
     4501 tccaccgccg ccgccgccct tgtcgccgcc attgcctttg tcgccgccgc gtccatggcc
     4561 gttgccggag gagatcgcgt cgccaaggtt ggccagggac gagctccctg gagtctgggt
     4621 ctgcaccgcc accgacttgt ctcccggctc accgaaatgg tggatcgcca ggggcgtcag
     4681 ggcggccagg accattcccg acagcatgta gcgggtcttg gaacgggagc tctgggtgcc
     4741 gatactcgcg acgtttcccg cgagggagcc gccgccgcgt tggaaggttc cgcgggcgag
     4801 caagcgatcg ctcgtgcgga acaggatcct ggcgtccgag gccgattgcg tggccgcggg
     4861 ggtcgcgctc gcgagcgccg cgtcggatcc cggatggtag ctgggatacg agggactcgg
     4921 caccgcgagc gcggctccgt acaggagcac ggaggccagc gcagcgagcg cgattccctt
     4981 cagaaacttc gggtaaagct gcatatgcag aacctctaag gccgccacat ggaagcagcc
     5041 tgaacggggg tccctggggt cgagactcgg aggggtcctg taattgcttc cctaggaccc
     5101 ttggaccggt gtaaatcgag gccatcacct gtcttcagct tccgaatccc ggcggcaccc
     5161 cggcccccac gtcgggccgg gctccggccg atcctttggc catcgttcgg ggcggtaggt
     5221 acttacccgg ctgaccgcgc ccgccatgcc gtctctcctc tcgttcccgg aggagcgttc
     5281 gagcgtcact gcatgagccg tgccccggac gggttccggt tttctcgaaa tcgcgggagg
     5341 aagggtgagt cggtggaact acccggtcgt gatcgtgggg ggaggagccg cggggctgat
     5401 ggccgcgctc tttgcgtccg aggcgggcgc gcgcgtcctg ctcgtggagc ggacgcgcga
     5461 cggcgggcgg aagatcgtga tgagcggcgg ggggcgctgc aacgtgctgc cctcgacgct
     5521 cgatccctcg cggttcgtga cggagtcctc cccgaacacg atgcgaaagc tcctcctcgc
     5581 ctggccgctc gacgagcagc ggcgcttctt cgaaacggtc gtggggatcc cgctcgccct
     5641 ggaagcggaa accgggaagc tgttcccggt gtcgaaccgt tcccgcgacg ttcgggacgc
     5701 gctggtggcg ctcgcggcac gccgcggcgt ggagcgccgg ttcgacacgc gcgtgaccgg
     5761 cgcggcgcct gcctccgatg gggggtggcg cctcactgtc gaagacggtg agccgattcc
     5821 cgcgtccacg gtcgtgctgg cgaccggggg cctgtcggtg ccggcgaccg gtagcgacgg
     5881 ggcggggctg gcgatcgcac agcggctggg acacgcgatg cacgacacct atcccgcgct
     5941 cacgccgctc gtcgcgcagc cccatcgctt cgccgcgctc tcgggaatct cgctaccggt
     6001 ggcgctggaa gcgccgctcg gtcccggaaa gcgcttccgg tccgagggag gattcctctt
     6061 cacccacaag ggctacagcg gccccgcagt gctgaacctc tcccacctgt gcgtgcgcgc
     6121 gcgcctggag gggcgcgagc agccggtgtt cgcgcgctgg acgccgctcg atgaagccgc
     6181 gtgggaagcc gagctgcgcg agggggaggg taccctgctg gccgccctcc gcgcgcggct
     6241 gcccgcgcgc cttgccgaga tgctcctcga ggaatcgggg gtggacggct cgatcgcgct
     6301 ggggcagctc cggcgggagg accgccgccg tgtcgtcctt gcgctcacgc gcttcccgct
     6361 cttctggacg ggcgacgagg gctacggcaa agcggaggtg acgggagggg gcgtgtcgct
     6421 ggccgaagtc gagccgcgca cgctggagag ccgccggtgc ccgggactct tcctatgcgg
     6481 ggagatgctg gacgcgttcg ggccgatc
//
LOCUS       DASSLZ010000246        31645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1089730, whole genome shotgun sequence.
ACCESSION   DASSLZ010000246 DASSLZ010000000
VERSION     DASSLZ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 31645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31645
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089730"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            516..1514
                     /locus_tag="VFM00_09265"
     CDS             516..1514
                     /locus_tag="VFM00_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HET7498478.1"
                     /translation="MTIIERLQTNGICRTGSPARGYRYRSAKGAVSREDLARIDALKI
                     PPAWTRVAVSPSPKSAVQAVGQDAAGRWQYLYHEAHAARRERNKQERLVRFIAALPRM
                     RRAVARDLRRRGLPREKVLAGILKILGTCFLRPGNQVYTAQNGSYGLATLRRQHVSVA
                     GDTVVFDFMGKSKKRQRRELVDPGIARLVRALLRYPGEVFKFQSEDGSMADVHTGHIN
                     AYIKEVMGEQFSAKDFRTWAGSLLCACALARAGVERAHTQHERKKRIAAALREVADHL
                     GNTPAVCRSSYVFPAVLRKYEQGRVIGTYFEDVAELSNGNARRVERSERALIELLR"
     gene            1511..3382
                     /locus_tag="VFM00_09270"
     CDS             1511..3382
                     /locus_tag="VFM00_09270"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7498479.1"
                     /translation="MSPRPARPGAKLVVIRPGDGVPTRNRRSVRGQARSERDPAEPRP
                     DGSAPTDLLILHIGSGRLTPEAMTAAAKTVFEPRGLTHRTVTVEAGRGAEASVRRHVA
                     RAIREGCTRIVAAGGDGTVSMVAQCLARRRKRDVPVSLGILPGGTTNVLARELGIPLD
                     LEQAMAVIAEAGQVVELDAILVGRRYVFTQVGLGPDALMIRDTTRERQEKLGRLAYVL
                     TFVKRAFGFRPRWFTLRLDGVVVRERAWQIVAANVGTVGAPPFTWGPRIDPTDGILDL
                     CIFNVNRKRDFGKLLWRALTSRHRRDGNARFYRVKDRAVVESSQPVLVQGDGEIIGRT
                     PVTLRLVPAVLRILVAKPVAGVVPTEPAAAAAEAAPLAEDRGVVASEEPAAASATAAA
                     PATASAPAAASTPAVGPNAAPEAAPTVTEDVALMIGERSTTWPLQGIFRHPLAAIEAY
                     DAAIFLHLNNISFGKGVDRGLEALSRFLHYGEGWAVVVLLILFVNLRQGLEAAVVALP
                     ALWATMITVNVVLKPIFRRRRPFIAFVKARVIGTRPRDFSFPSGHAAAGFAGAVLLGM
                     FLPRWAPLFYLFAIAVSFSRIYLGVHYPSDVLMGAFAGSVLAVVYRALIHLLVAAGA"
     gene            3394..4266
                     /locus_tag="VFM00_09275"
     CDS             3394..4266
                     /locus_tag="VFM00_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017310770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7498480.1"
                     /translation="MPQKTSPKKAQRPPQKQRRQPGRERLMKPRPRYDDPARRPCGRL
                     QGKAALITGGDSGIGRAVAVAFAKEGADVAIVYLEEHEDARETQRLVQEQGRRALLMA
                     GDVGDESFCREAVRRTVAELGKIDVLVNNAAEQHPQERLEKITEEQLVRTFRTNVFSM
                     FFLTRAALPHLKSGASIVNTTSVTAYRGSAHLIDYASTKGAIVSFTRSLAQALADRKI
                     RVNAVAPGPIWTPLIPSTFPPDQVKTFGSDVPMGRAGEPVEVAPHYVFLASEEASYTT
                     GQVLHPNGGEIVNG"
     gene            4263..4661
                     /locus_tag="VFM00_09280"
     CDS             4263..4661
                     /locus_tag="VFM00_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7498481.1"
                     /translation="MSADDAPREADRSLSILVVDDDPDSADSLALLLEHWNHAVCVAY
                     DGAHALETFRRQRPSIVLVDIGLPDMDGYDVARALKAEEHPATLVALTGFGQDGDRRS
                     SKEAGFDHHFVKPIDLRSLRELLDAAIAKS"
     gene            4798..5220
                     /locus_tag="VFM00_09285"
     CDS             4798..5220
                     /locus_tag="VFM00_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498482.1"
                     /translation="MSLLLEICFVIVTIAVVAIAIATVRALQRVGKTTEKVNELSEEV
                     RGWIAQLKQVTSGAEELVASAKEVVPPVRRVVDRFEAIGVRTAQLSDAILEEVERPVM
                     TAVAVARGVRTGTAYFFERITNRFHRGRAATNGGYADE"
     gene            5213..5623
                     /locus_tag="VFM00_09290"
     CDS             5213..5623
                     /locus_tag="VFM00_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET7498483.1"
                     /translation="MNENPYLNEESRGGASGMSGFVLGAIVGAGVALLFAPAAGTDTR
                     RKLGETAKRLGSKAQEMGSAARDKMQEVGSAARDKVQEGTDKLHERFGSGTSSTTPGQ
                     GFEAGRREPVPSTGSTTNPGQTGRTGTTPGRSNP"
     gene            5773..8463
                     /locus_tag="VFM00_09295"
     CDS             5773..8463
                     /locus_tag="VFM00_09295"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HET7498484.1"
                     /translation="MQRPSARDLIRAAGVAALYYGAGRLGLALASVNSSATAVWPPTG
                     IAIAALLVFGYRMWPAILAAALLVNVPVSGAGVVSAAIAAGNTLEALAGAWLVHRFAG
                     GAAAFEEPRRIFAFAGMALLATAVSPTVGVTAVAGAGAVPWSGYGPMWLTWWLGDLSG
                     ALVVAPFLILWLSRPRVRWKLRRSGEALALFASLILIASLVFGGASSFPGGAPPLEFL
                     CVPVLVWAAFRFSQREASTATVLLSGIAILGTLRGAGPFARADVNQSLLLLQSYTSVS
                     AVLVLAVTAALTGRRRDEAGVRRLAGELERRVEARTAELKRAVQTLREEARERERAQE
                     ALGTSEARLREAQRVARVGSWEWDIERDSLWWSEELYGIYGVDSASFPATLESFIALV
                     HPEDRPLVRRVIEAAHRNRQPFKIEHRIVRPDQTVLTMSARGRVVTDEQGRAVRMLGT
                     GQDVTDEKRAEEERSHLLVEREARRQAEEANRLKDEFLATLSHELRTPLNAIVGWANL
                     LREGNLDPATTTRAVETISRNAQIQSRLISDILDISRLIAGQLDFKLQMVSLENVIEG
                     ALDTMRPAAEANGVRLEANLAPGIGSVLGSPDRLQQVVWNLLSNAIKFTPKGGRVWIV
                     LAARESSAVIRVEDEGIGIDPDFLPHVFERFRQKNPSSSRRHGGLGLGLAIARHIVEF
                     HGGTITAENRESGRGARFVVTMPLVEATEAARPGEGDAAGRSAAGADRRPAPSGAPRA
                     AAPAEGAEAEPAPNPLAGVRALLVEDEADSRELLARLLTSCGAEVRVAGSAAEALGLL
                     VREHPNLLISDIAMPGETGYDLIERVRALPPEAGGRIPAIAVTAYTGAEDARRALRSG
                     YQAHLSKPVQLRELVRLVADLRDGSSLPLESPGPSGPHGR"
     gene            8453..9289
                     /locus_tag="VFM00_09300"
     CDS             8453..9289
                     /locus_tag="VFM00_09300"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET7498485.1"
                     /translation="MAADGRGRVARTGEPLGLMGVFAHPDDETLGVGGVFARYAAEGV
                     RTSLVTATRGDRGRFRGIRHGEGPEHPGPEALALIREQELRAASRVLGIEDVTLLDYG
                     DGRLDEADPREAVARIAAEIRRVRPHVVLTFAPDGAYGHPDHIAICQLATASVVAAAS
                     GDAPHTVSKLYYMATTQARWDAYQAAFKKLVSLVDGVERQAQPWPEWMVTTTVDTREQ
                     WPTVWRAVVCHDSQVGGYEKLRHLAPEHHEALWGRQQFYRVLSVVNGGRGMETDLFEG
                     LR"
     gene            9286..10836
                     /locus_tag="VFM00_09305"
     CDS             9286..10836
                     /locus_tag="VFM00_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET7498486.1"
                     /translation="MTTKTDGGAASAGANQAARRQAPLAMDAEAFRAAGHRLVDRVSD
                     LLASVPERPVTRGEAPSGVRAALDLNGSLPEEGCDPEALLTTTAGLLFDHSLFNGHPR
                     FFGYITSPPAPVGILGEMLAAAVNANAGAWILAPAATEIETQTIRWIAELIGYPASAG
                     GILVSGGNMANFVGFFAARAAQAPWPVREKGAAGGPPLRAYGSAETHTWIQKAADLAG
                     LGTESVRWIETDGAMRMRVDALRRKIREDRERGDVPFLVVGTAGSVGTGAVDPLPEIA
                     ALCREEKVWFHVDGAYGGLAAAVPEAPEDLRGLALADSVAVDPHKWLYAPIEAGCALV
                     RDPARLRAAFAYHPPYYRFGEEGLNYVEYGPQNSRGFRALKVWLALRHAGARGYRAMI
                     AEDMRLSRVMAERVDAEPDLERFTQSLSITTFRFLPRDLRERRGEADVAEYLDRLNQD
                     LLDRIQNGGECFVSNAVIGGRYVLRACIVNFHTGEADVAAVPVIVARVGREVDAALRP
                     AALRAARS"
     gene            complement(10859..11470)
                     /locus_tag="VFM00_09310"
     CDS             complement(10859..11470)
                     /locus_tag="VFM00_09310"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HET7498487.1"
                     /translation="MSRAQRIWWIAGVTALLAGAGFGYFALRPLPRDQDAANPDAGYL
                     VDSSAASIAESLAAPPPRQPPYGIDSGAVMTGGIPVGGEEPAADSPGEDGATRLGPVL
                     LDTPRPNQIAQSPLWIAGRARGSWFFEAEFPVRLLDARDSVVARGVARATGDWMTPEY
                     VPFSLTLDFEPPDTEEGTLILERSNPSDLPEHAASVRLPVRFR"
     gene            complement(11479..15603)
                     /locus_tag="VFM00_09315"
     CDS             complement(11479..15603)
                     /locus_tag="VFM00_09315"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7498488.1"
                     /translation="MADRNGATPEEARDRDRLGDPLDGFHPTVARWVRERIGVPSPAQ
                     AEGWPRIRSGAHTLIAAPTGSGKTLAAFLWALDGLLRQGGALPDETQVLYVSPLKALS
                     NDVQKNLQAPLAELTLLDPECAAVRVLVRTGDTPASERASMGRRPPHVLVTTPESLYI
                     LLTSAGGRSLLRTVRTVIVDEIHAVAGSKRGAHLALSLERLEALAGGPIQRIGLSATQ
                     KPVTDVAELLVGVGRDCAIVDTGHRRALDLGIELPDAPLATVCSHETWDDITKRMGEL
                     IGEHRTTLVFVNTRKLAERIAARLTKVLGEEQVTSHHGSLSRERRLDAERRLKQGSLR
                     ALVATASLELGIDIGDVDLAIQIGVTPAISVFLQRVGRSGHGLGRVPKGRIFPLTQDE
                     LVTAAALLEAIRSGELDRTPMPRRSLDILAQQIVASCVPETWEEERLYDTLRRAWPYQ
                     DLARAEFDDVVRLHTAGRAALLHRDGVNGKLRATRRASITALTSGGAIPDTGQYRVVV
                     EPEGTFVGTLDEDFAVESNGGDVFQLGNASWQVLRVEPGIVRVADAHGAPPTLPFWLG
                     EAPARTRELAAAVGRVRVRGRDAAWLTNDLGLSAEAATQLSDYLREGERSLGAIPTPE
                     RVVLERFFDESGGMQLVVHAPFGGRINRAWGLALRKRFCRGFGFELQAAANEEAIVIS
                     LGPHHSFPLDDVFQFLHPATARDLLVQALLDQPMFGTRWRWNLTRALLLSRTQGGGKR
                     VPTPLLRMRAEDALIQAFPQVLACPETLPPGELPVPWEHPIVRQTIEDCLTEAMDADG
                     FLELLRGLRSGAIERVAVDTPEPSAFARGILNAAPYAFLDDAPLEERRTQAVSSRRFL
                     DPETADTLGALDPDAVARVREQAWPDPENAEEVHEALLWMGYVTEREAEISGWMPWIE
                     ELASQRRVLRDAPAGEEARWRAVEAPTDPKTVLRGRLEALGPVFVGAGEGFLPPSAEE
                     YLLALEVEGRVLRCRLDGRQAWCDRRLLARIHRETLERLRREIEPVTAAGFWRFLACW
                     QHAAEGYRVEGPRGVVEVARQLAGFEIPAAAWESSVLPARVEGYRQEWLDQATLSGEL
                     AWGRLWGGGRSPIRSTPVALLPREELDLWLSLAAPAAARAPAPADDGEAVGTAEGEID
                     PREISTYADVLLSVLAARGASFAQDLERHSGLLPAHVEMGIAELIGRGLVTCDSWSGL
                     RRLITPPSRRRGALKRARFAPPGRWTVLRVPREAGDDPVAAGRLAAGAPFTLAAEAEI
                     EFLARRLIDRYGVIFRRLLDRERLPVRWRELVRVYRLWELRGDVRGGRFVQRFAGEQY
                     ALPEAVDLLRRLRRSDAGSGAGVRVAAADPLNLDGILTPEPRIPAAARRRVLVA"
     gene            complement(15596..16237)
                     /locus_tag="VFM00_09320"
     CDS             complement(15596..16237)
                     /locus_tag="VFM00_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420238.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HET7498489.1"
                     /translation="MTPRSTTGASALEFLPARLTMASLREAARGCRGCPLYRNATQTV
                     FGEGPRTARVVLVGEVPGNDEDLAGHPFVGPAGRVLDEALEAARIARDETYVTNVVKH
                     FKWEPQGKRRKHKKPSAREIAACLPWLEREMELIRPDVLVCLGATAAQAIVARDFKVT
                     AMRGRIVPTALAPRTVATVHPSSILRQRGSDERRREMARFVADLKIVAGLIHG"
     gene            complement(16332..16796)
                     /locus_tag="VFM00_09325"
     CDS             complement(16332..16796)
                     /locus_tag="VFM00_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498490.1"
                     /translation="MRFDHRAAAVALCLTVAAFTGACQKNGTQTSSTSGTEGTTPSAQ
                     GVQVARVDLGTSLANDNTVATQTNTFLPNQTIYASVVTNGSAPSAELKTRFTYQDGQV
                     VDESTRTIAPSGQAVTEFHVSKPDGFPVGKYKVEVFVNGTSAATKEFEVKST"
     gene            complement(16819..17073)
                     /locus_tag="VFM00_09330"
     CDS             complement(16819..17073)
                     /locus_tag="VFM00_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7498491.1"
                     /translation="MADDKKQSRPDFSNVQSGSSSTAPSPAQDGGAGATYVVKPGDSL
                     SKIARHHYGDAGQWHKIYEANKDRIHDPDHIEPGWTLRLP"
     gene            17234..18025
                     /locus_tag="VFM00_09335"
     CDS             17234..18025
                     /locus_tag="VFM00_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET7498492.1"
                     /translation="MILIVGAHPGDESAGASSVLFSPDDVSVLHATDGAPRDPAEARA
                     NGFETREAYAAAREAEARAALAFAGIPPSRRVTLGQIGREASLHLAALTRAVLATIER
                     LRPEVLLTHAYEGGHPDHDAVAFATHAAVALRRASGSPVPALSEMASYHRGPGGGFES
                     GVFLGGAAPEDIVRRLDAAARARKLQLLARYRTQWSFLRRFRVEEERFRPAPFYDFTR
                     PPHAGRLWYEPFPWGMSAARFGDLVRYALLELGLRQAAEPVSSTS"
     gene            complement(18030..19808)
                     /locus_tag="VFM00_09340"
     CDS             complement(18030..19808)
                     /locus_tag="VFM00_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498493.1"
                     /translation="MIVYGDPSRREPLERCVETLNLMLEQAQTADLRPARVRGLVERA
                     GELEQALFDDSEEVGTLRARSRDLAAARRVTTLAATAFDRSFSGTPGEAARAVGASCR
                     ALAPLRRRCGTVDVKVPEGFAFYGLMPQSYRDQALRWALEHENAPDRRVLVVGIRTIG
                     TTLSAVVMATLRARGFRAKRITVRPSGQPLERRLEVPLPGAPFGIVVDEGPGLSGSSM
                     LAAAEAMRRTGIDDIALFPAHGNGPGGAPAEARSRWRSFRSYPAMDSPQRFGERTLAE
                     ELWTGVRTAFGDLVRVTEVGGGMWRAAHYGGERAWPAVLRPLERPKLLCASQDGGRVL
                     FKFCGHATAPGMERTLAAQIARRSAPLAALGFAPRLLGEMHGFVAWEWVDGRPLGIQD
                     ASLPFLVRMGAYIARAALPPLSPEASHEARERTAAMLRANAGEILGNEAVRAAEAEFR
                     KGRDEPSAPGAGDGHLAPHEWIRSRSGAIVKTDVGGHDVDHTWTGPQPVIWDLAGALE
                     EWDLHGAQAEAMLRGYGDAGGPPPAPATLHAYRTAFAAHRAGQTAFFAEVERGAPERA
                     RLELARERWRAQLTRCLVADPAGAAR"
     gene            complement(19805..20356)
                     /locus_tag="VFM00_09345"
     CDS             complement(19805..20356)
                     /locus_tag="VFM00_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7498494.1"
                     /translation="MTRFYLVRHASHDLLGRVLAGRMPGVHLNARGRREADWLRERFQ
                     SVPVDALVTSPLERSLETAGPIATALGLAPEIEPALGEMSFGEWTGRSFEELEAVPAW
                     RAFNTCRSVTPVPGGETMLQTQVRAVAALERQRERHSDGAVLAADGATLVRLNDAPPV
                     FETLENPASLNGSAAPAAPEVAR"
     gene            20694..21545
                     /locus_tag="VFM00_09350"
     CDS             20694..21545
                     /locus_tag="VFM00_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498495.1"
                     /translation="MLASLPGTDPTDAAQPAVMGPAETRTVPASWLGHSGRLRAVIDT
                     PVSLESQLIADSIQAANLTPGLHDLGIVTPDGDAFYVVSLLPYATKEGKRLNGYYVGD
                     WPQGAPTGRYAPPAGFIEVTPENEATPVSQSFKLQDFLTHDQQGVWPKVLVLKPKLVD
                     KLELISAALAERGLPSHLHVMSGFRTPQYNEQGVGPKGGRASLSRHMYGDASDVFVDE
                     NGDGMMDDLNGDGRTTVADARVLFQVAESVEAAHPDLIGGLSPYPSNGVHGPFVHVDT
                     RGFKARW"
     gene            complement(21594..22682)
                     /locus_tag="VFM00_09355"
     CDS             complement(21594..22682)
                     /locus_tag="VFM00_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7498496.1"
                     /translation="MSHISSSSGFASREPASPPMDTRHGDPILDPSRAARILQNAISD
                     SGWTVQEVRSVDVLSERPDNRRTLRFRVSARRAGSPFSDVTWHGLQYRSGEGDRMLPV
                     LRFLRAAAAPEIRVPTPLGYAREHKLLLQTTMDGRPLAGVLEDPIESRVAASLKRLGR
                     ALASFHAIRSPGPSARSTERPSFGRWDAASEAQNLDDVVTRLRQSGIDARAADRLRDG
                     AGVLKAELADDGGVSRSPSMVHRQLRPTHVIFGSDGISVVDLDDASFAEPELDLGTLI
                     AHMMLSDLKRGGGTKMAPARAEALRAGYLGGGVLRPNRLAAYTSSALLRLAADEKTKN
                     GGVGAPDWTRLTAAVIEEALPELSGARA"
     gene            complement(23099..23737)
                     /locus_tag="VFM00_09360"
     CDS             complement(23099..23737)
                     /locus_tag="VFM00_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7498497.1"
                     /translation="MVRSPNFYRGLVLGAGLMYLLDPSRGRTRRARIRDQITGALGDS
                     RAFLDDAVHDLQNRGNRAGGLVARAAAAAPSGPDDAIVERVRAKLCRYVTHPGAVSVE
                     ARDGSVVLNGPILRGEAESLIGAVSSVRGVREVLNRLEPHDAEDEVSEFRGSRRHRVF
                     DPVPAMPAGLQLLAGVLGATAVAYGAGRIAARVRAARVDELEREMPEYAMLR"
     gene            23967..25439
                     /locus_tag="VFM00_09365"
     CDS             23967..25439
                     /locus_tag="VFM00_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7498498.1"
                     /translation="MSRSDARSFFDGRTFDDFLFRPGKGRVGSRREVRLTGRFTGAIA
                     LELPIVSANMDSVTEGPMARALALEGGIGVVHRALPIGEQAAEIERVKRSHGWVVEEP
                     LCLPRGASLREARDVIRRHGITGILIEEERGSRALAGLLSNRDIPWGPGHEAEPVERF
                     MTPFDRLVTGAPGIALEEAERVLYERRIEKLPLVDATRRIRGLITKSDIQLARERPES
                     TKDAKGRLRVAGAIGARGDFLERAEALLDAGADALVVDIAHGHSEVMEQAVQALRARF
                     GSVPELVCGNVGTAEGARFLRDLGAAAVKVGIGPGRGCRTRLETAAGVPQLQAIHEAR
                     MALGEGTPIIADGGIRHDKDLFLALVCGADAVMLGSMLSGTDEAPGRVIEDPSSREKR
                     KIYRGMTSPQAVFQALYDDTDPVELEQALDTPSEGQEIQVPYRGGVRDVLHRVRGHLR
                     SAVSYAGEESLACVRERVLANPSDNLIALSAASRRESFDR"
     gene            25694..26383
                     /locus_tag="VFM00_09370"
     CDS             25694..26383
                     /locus_tag="VFM00_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7498499.1"
                     /translation="MAQIDRRSETEQEEQRRLDEERRVRQAETGMGPRRETSEEYRER
                     RRRMERDQGAWPYAGPLSATGYRPPGYMGYPGYPSYPWSRFSHGMDPSYEDWQGPGFG
                     THTPGGWEPRVETFRRGDDLIVRVELPGWDKDEVSVFVEEEDLIVEGAREEGAEGDPV
                     RRDEERRKDERRGEWSGSRYAAERFSTRVSLPAGADPGDLKAKFKNGVLEVKIPMPER
                     RTERRTVKIDT"
     gene            26424..29456
                     /locus_tag="VFM00_09375"
     CDS             26424..29456
                     /locus_tag="VFM00_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET7498500.1"
                     /translation="MKIDLNRFRAPLRFQAGVFLDRKLFRGMPAPKAASEPDHGRRLP
                     ARLWAAAGIGLILFAHWEMSTAALESRLFSSLASRMTYRVEPGKSPRITFPKSGPFDT
                     RLGYSRVPEFAKRLESRGFEYQSQARFTKTMALVADLGVPPPYPEPPVAGLVIDGSGG
                     EPLYDAMGTRRTFARFEQIPPDVVKSLLFIENRELDRFDSPYANPAIDWSRSAKAMVI
                     YAASRLGLPTPVEGGSTLAVQMEKYRHSPSGRTSGAVEKLRQMIGASLRVYRQGPRTV
                     EERRRIVLDYVNTVPLGGTPRYGEVFGMDEGLRVWFGTEPEKVYAALAKPRVTKARAR
                     ALKEVLALVCAVRAPSRYLVSDRGALEDRVDKFVKLMASRGLLEPKLADAVVKTRLRF
                     ADVSTQRYRTQTDKATVLVRMRLRNLLGVPNLYDLDRLHVNVKTTVNAGLERDVTAFF
                     HRMNDEGYVDSTGLKAAHLLLDGDPDRVVYSMILRERTPTGDVVRVHADNVAGPFDVN
                     EGTKMELGSTAKLRTLVHYLNVMSDLHQTLSALPDDVVRDRAKASRDPLTQWAAETVR
                     DNPGITLSDFLSKSLDRKYSASPYELFFTGGGLLTFENYDKSENSQILPIREAVVHST
                     NLVFIRLMRDLVRYHEARLPYDADKILGPKDTPERTWMLRQVAAEEGTPEQMAWLFRT
                     RNRSAQDNRLRARIERDAFARMTPYWRRLGFPFSHLVPSYATAIGSSSDQPAALADLM
                     GVLVNDGVRRPPALIQEIAFASDTPYETRLDAPAPQGGDLLVPPEVARAARSVLGAVV
                     ERGTAARLHGAFVDSTGAPIWVGGKTGTGDNRFDTFGRGRRLISSRAVSRTAAFAFCL
                     GDRYYGVVTASVLGPEADQYTFTSVLPVEVLRRLAPVIEKRLRLVGGTAPPGATVAAV
                     QDGAAESDDVSTDSTAVPASPANVGAAPAPDTAPAAPADIKSGTPAKTGEADDARKIH
                     DDAMKNRDAQRAKDRRQNERRRQWDWESPSRTSAGGRL"
     gene            complement(29453..29929)
                     /locus_tag="VFM00_09380"
     CDS             complement(29453..29929)
                     /locus_tag="VFM00_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7498501.1"
                     /translation="MSNRTSAGLLLFRHRGGSLEVLLVHPGGPFWKDKDWGAWSIPKG
                     EFEDDEPALDAARREFEEETGSAAAGPFTPLRPLRQKSGKVIHAWAVEGDLDASAVRS
                     NTFTLEWPPRSGRQRTFPEVDRAAWFAPEEAARRIHPGQAPFLTELIAHIARGGRS"
     gene            30204..31580
                     /locus_tag="VFM00_09385"
     CDS             30204..31580
                     /locus_tag="VFM00_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7498502.1"
                     /translation="MTPQPDRMAAVERAVESRLERNLEELARLVAQPSIAAQNVGMDA
                     CAELAAGMLRERGFSVEFPRVPGFPVIVAERHGASDRTLLIYNHYDVQPPEPLELWTS
                     PPFEATRRGDRLIARGVSDDKGHFTARLLAIDALLAERGDLPCRILWVLEGEEEIGSP
                     NLPAFVRDHKERLAGDACLWEFGYVDPGGVPVLHAGLRGICYVELSVETAARDAHSGV
                     GGSIFPNAAWRLTWALASLKGPDERIRVPGHYDSVRPPSSRDRELMEAMPETAGEYRE
                     LYGVTRFLRGLEGGAELRIAEVFEPTCTICGLDSGYQGAGSKTVLPARASAKVDFRLV
                     PDQTPAEVLQNLRAHLDREGFPDVTIRFLGGNPAGRTDPDHPFLRLVAETAIPAFGVP
                     MRIVPMIGGSGPNESFIRYLGVPVATAGFGYPDTRAHAPDENLLVSLYEKHAKHAARL
                     IDAFASGS"
BASE COUNT         4656 a        11144 c        11273 g         4572 t
ORIGIN      
        1 cgctcggtct cgttggcgct gggttcgctt tggcgctggg tcttggtggc cacggatctc
       61 gagggcacaa aaaaacgggc gggcagtgcg ctcgtttgga tttcatctcg gtgaggcgaa
      121 gggttcactt tcgtgatctt cgatctcacg ttctgaattc cacgagcacc ctgcaccgcc
      181 caacaagcgg ccgcttggcg catctctcta tgtcaacggg cccggtcggg gaccgctgat
      241 cgatcagcgg tcgttctcgc atcaaagcga gaagagtgcc aatcaggggg cgtttccgcg
      301 tggagcgggg cgcgctgcga catcgatggt tacgcggccc gcgcgagtaa gggaatgcgc
      361 ccggcgccgg cgacgcccgg gcgattctac aaatgacaac gacgagtgcg cctcgacggg
      421 agcgagaccg tcgcgcggac tcgccgtctt cgcgttcacc tcgcggtggc acacctgttg
      481 cgccgcgctc ctgctcagtc gagggacctg tgccaatgac catcatcgaa cgactccaga
      541 cgaacggcat atgccggacc ggctcgccag cgcggggata ccgctatcgc tccgccaagg
      601 gcgccgtctc gcgtgaggat ctggcgcgta tcgacgcgct gaagattcct cccgcgtgga
      661 cccgcgtcgc cgtttcgccc tcgccgaaat ccgccgtgca ggcagtgggg caggacgccg
      721 ccggccggtg gcagtacctc taccacgagg cgcacgccgc ccggcgggaa cggaacaagc
      781 aggagcggct ggtccggttc atcgcggcac tgcccaggat gcgccgcgcc gtcgcgcgcg
      841 acctgcgccg ccgggggctt ccgcgcgaga aggtgctggc cggaatcctc aagatcctgg
      901 gcacctgctt cctccgcccg ggcaaccagg tctacaccgc gcagaacggg agctacggcc
      961 tcgcgacgct ccggcgccag cacgtcagcg tggcggggga caccgtcgtg ttcgacttca
     1021 tgggcaagtc caagaagcgc cagcgccgcg agctggtcga tccgggtatc gcgcggctcg
     1081 tgcgagcgct gcttcgttat ccgggagagg tcttcaagtt ccaatccgag gacggctcca
     1141 tggccgacgt gcacacgggg cacatcaacg cctacatcaa ggaggtcatg ggcgaacagt
     1201 tcagcgccaa ggatttccgt acgtgggcgg gatcgctcct ctgcgcatgc gccctggccc
     1261 gcgccggggt ggagcgagcc cacacccagc atgaacggaa gaagcggatc gccgcggccc
     1321 tgcgcgaagt cgccgaccac ctcggcaaca cgccggccgt ctgccggtcc tcgtacgtgt
     1381 tcccggcggt tctccggaag tacgagcagg gtcgagtgat cggaacgtac ttcgaggacg
     1441 tggccgagct ctccaacggc aacgcgcgcc gcgtcgagcg aagcgagcgg gccctgatcg
     1501 agctgctccg atgagccctc gtcccgcccg ccccggcgcc aagctggtcg tgatccgtcc
     1561 cggagacggg gtcccgaccc ggaaccgtcg ctctgtccgg gggcaggcgc gttcggagcg
     1621 cgaccccgcc gagccgcgtc cggacggatc ggctccgacc gacctcctca tcctccacat
     1681 cggctccggt cgcctcacgc cggaggcgat gacggcagcc gccaagacgg tgttcgagcc
     1741 gcgcggcctg acgcatcgga cggtgacggt cgaggccggg cgcggcgccg aagcgtcggt
     1801 gcgtcgccac gtcgcgcgcg cgatccgcga gggctgcacg cggatcgttg ccgcgggagg
     1861 cgacggcacg gtcagcatgg tggcgcagtg cctggcgcgg cggcgcaagc gcgacgtccc
     1921 ggtgtcgctc ggcatccttc ccggaggcac cacgaacgtg ctggcccgcg agctgggtat
     1981 tccgctcgat ctggaacagg cgatggcggt gatcgccgag gccggccagg tcgtggagct
     2041 ggacgccatc ctcgtgggcc ggcggtacgt cttcacgcag gtgggcctcg gcccggacgc
     2101 gctcatgatt cgcgacacga cccgcgagcg ccaggagaag ctgggccgcc tcgcctacgt
     2161 cctcacgttc gtcaaacgcg ctttcggctt ccgcccgcgg tggttcacgc tgcgtctcga
     2221 cggcgtcgtc gtgcgcgagc gcgcctggca gatcgtggcc gccaacgtgg gcacggtggg
     2281 ggcgcctccg ttcacgtggg gtccccgcat cgatcccacc gacggcatcc tcgatctctg
     2341 catcttcaac gtgaaccgga aacgcgactt cggaaagctg ctctggcgcg ccttgaccag
     2401 ccgccaccgg agggacggga acgcgcgttt ctatcgcgtg aaggaccgcg ccgtcgtgga
     2461 gtcgagccag ccggtgctgg tgcagggcga cggcgagatc atcgggcgga ccccggtgac
     2521 gctgcgcctc gtccccgcgg tcctcaggat cctggtcgcg aagccggtgg ccggcgtcgt
     2581 tcccaccgag cccgccgcgg cggcggcgga agcggctcca ttggcggagg accgcggggt
     2641 ggttgcttcg gaagagcccg ctgcggcatc ggcgaccgct gcggcgccgg cgaccgcttc
     2701 cgcgccggcg gccgcctcga cgccggccgt gggcccgaac gccgcacccg aggccgcgcc
     2761 caccgtgacc gaggacgtgg ccctgatgat cggcgagcgc tccaccacgt ggccgctcca
     2821 ggggatcttc cgccacccgc tcgcggcgat cgaggcgtac gacgccgcga tcttcctcca
     2881 cctcaacaac atctcgtttg gcaaaggggt ggaccgcggc ctggaggcgc tctcgcgctt
     2941 ccttcactac ggcgaggggt gggccgtcgt ggtgctgctc atcctcttcg tgaacctgcg
     3001 ccaggggctg gaggccgccg tcgtggcgct tcccgcgctg tgggccacca tgatcacggt
     3061 caacgtcgtc ctgaagccga tcttccggcg ccgccggccc ttcatcgcct tcgtgaaggc
     3121 gcgcgtcatc ggaacgcggc cgcgcgactt ctcctttccc tcggggcacg ccgcggccgg
     3181 gttcgccggc gcggttctgc tcggcatgtt cctgccgcgc tgggcccctc tcttctatct
     3241 cttcgcgatc gcggtgagtt tctcgcggat ctatctcggc gtgcactacc cgagcgacgt
     3301 cctcatgggg gcgttcgccg gaagcgtcct ggccgtggtc tatcgcgcgc tgatccacct
     3361 tctcgtcgcc gccggcgcct gaccggagga acaatgcccc agaagacctc acccaagaag
     3421 gcgcagcgac cgccgcagaa gcagcggcgc cagccggggc gcgagcgcct catgaagccg
     3481 cggccgcggt acgacgatcc cgcgcgccgc ccctgcgggc ggctccaggg caaggcggcc
     3541 ctgatcacgg gaggggacag tggcatcgga cgcgccgtgg cggtggcgtt cgccaaggag
     3601 ggagccgacg tggcgatcgt ctacctggag gagcacgagg acgcgcggga aacccagcgg
     3661 ctggtccagg aacagggccg gcgcgccctc ctgatggcgg gcgacgtggg ggacgagtcg
     3721 ttctgccgcg aggcggtgcg ccggacggtc gccgagctcg ggaagatcga cgtgctcgtc
     3781 aacaacgccg ccgagcagca cccccaggaa cgcctggaga agatcacgga agagcagctc
     3841 gtccgcacct ttcggacgaa cgtcttctcc atgttcttcc tgacgcgcgc cgccctcccc
     3901 catctcaaga gcggcgcctc gatcgtgaac acgacctccg tgaccgccta tcgggggagc
     3961 gcgcatctga tcgactacgc gtccacgaag ggggcgatcg tctccttcac gcgctccctc
     4021 gcccaggcgc tcgccgaccg gaagatccgc gtgaacgccg tggcgccggg gccgatctgg
     4081 acgccgctca tcccctcgac ctttccgccg gaccaggtca agaccttcgg ctcggacgtt
     4141 ccgatgggcc gcgccggcga gccggtcgag gtcgctcccc attacgtctt tctcgcctcg
     4201 gaggaggcct cctacaccac gggacaggtc ctccatccca acggcgggga gatcgtgaac
     4261 ggatgagcgc cgacgatgct ccgcgcgagg cggaccgctc cctctcgatt ctcgtcgtcg
     4321 atgacgatcc cgactcggcg gacagtctgg cgctgctcct cgagcactgg aaccacgcgg
     4381 tctgcgtggc ctacgacgga gcgcacgccc tcgagacctt ccggcggcaa cggccgtcga
     4441 tcgttctggt cgacatcggg cttcccgaca tggacggcta cgacgtggcg cgcgccctca
     4501 aggccgagga gcatccggcc accctcgtgg cgctgaccgg ctttggccag gatggagacc
     4561 ggcgctcttc gaaggaagcg ggcttcgatc accacttcgt caagccgatc gatctccgca
     4621 gccttcgcga gctgctcgat gcagccatcg cgaaatcttg aggccgggag gccttggccg
     4681 cagcgcgccg ttggccttca tattgcacct cacccagaag agaaggggtt gggccaaagg
     4741 agcgacaccg agggtggttc caaccgcaag gccgattggg ccggaggagg atgaaggatg
     4801 tcgctgctac tcgagatctg ctttgtaatt gtaacaattg cggtagtggc catcgccatc
     4861 gccacggtac gcgctctcca gcgggtgggg aaaacgaccg agaaagtcaa cgagctctct
     4921 gaagaagttc gcggctggat cgctcagttg aagcaggtga cgagcggcgc cgaggaactc
     4981 gtggcctccg ccaaggaagt cgtgcccccc gtgcggcgcg tcgtggaccg cttcgaagcg
     5041 atcggcgtac ggaccgccca gctctcagac gccattctgg aagaagtcga gcgtcccgtc
     5101 atgaccgccg tcgccgtcgc gcgcggggtc cgaacgggga cggcgtactt cttcgaacga
     5161 atcaccaatc gttttcatcg cggccgcgcc gcgacgaacg gaggctacgc agatgaatga
     5221 gaatccttat ctgaacgaag agtccagggg cggcgcctcc gggatgtccg gcttcgtgct
     5281 cggcgcgatc gtaggcgccg gagtagcgct gctgttcgcc cccgcggccg gcaccgacac
     5341 gcgccgtaag ctgggcgaga cggccaagcg cctgggcagc aaggctcagg aaatgggcag
     5401 tgcggcgcgc gacaagatgc aggaagtggg cagcgcggcc cgggacaagg tgcaggaggg
     5461 taccgacaag cttcatgagc gttttggcag cggaacgtcg tccacgaccc cggggcaggg
     5521 cttcgaggcg ggccggcggg agccggttcc cagcacgggc agcaccacga atcctgggca
     5581 gacggggcgt accggcacga cgccgggacg ctcgaatccc tagtcgaggt tcgaacatag
     5641 gaaaaccccc cacaaggggg cggcaccgcg gaggtgtagg ccggacggca agggctcagg
     5701 ctcggccgta tgcgaactac acctttcgcg ggcctttttt ttcggtccaa atccgcatgc
     5761 tatcctgatc ggatgcaacg cccctccgca cgcgacctga tccgcgccgc aggcgtcgcg
     5821 gccctgtact acggggcggg gaggctgggc cttgcgctcg cgtcggtcaa ctccagcgcg
     5881 acggcggtct ggcctcccac aggcatcgcg atcgcggccc tcctcgtctt cggttaccgc
     5941 atgtggcccg caattctcgc ggccgccctc ctggtgaacg ttccggtgtc gggagccggc
     6001 gtcgtgtcgg ccgccatcgc cgccgggaat accctcgagg cgctcgccgg agcctggctc
     6061 gtgcatcgct tcgccggagg agccgccgct ttcgaggagc cgcgccggat cttcgccttc
     6121 gccgggatgg cgctcctggc cacggcggtg agccccaccg tcggcgtgac cgcggtggcg
     6181 ggcgccggcg ccgttccgtg gagcgggtac gggccgatgt ggctcacctg gtggctcggc
     6241 gacctctcgg gcgcgctcgt cgtggctccg ttcctgattc tctggctctc gcgtccccgc
     6301 gtccggtgga agctgcgccg ctccggggaa gccctcgctc tgttcgcgtc gctcatcctc
     6361 atcgcgtcgc tcgtgttcgg gggcgcctcc agcttcccgg gaggggcgcc tcccctcgag
     6421 ttcctctgcg ttcccgtgct ggtctgggcg gcgttccgat tctcgcagcg tgaggcgtcg
     6481 acggcgacgg tgctgctgtc ggggatcgcg atcctgggca cccttcgcgg cgccggaccc
     6541 ttcgcccgcg ccgacgtgaa ccagtcgctg ctcctcctgc agtcctacac ctcggtctcc
     6601 gcggtgctcg tgctcgctgt cacggcggcg ctcaccgggc gccggcgcga cgaggcgggc
     6661 gtgcgccggc tggccgggga gctggagcgc cgcgtcgagg cccgcaccgc cgagctgaag
     6721 cgcgcggtgc agaccctgcg cgaggaggcg cgcgagcgcg agcgggccca agaggcgctg
     6781 ggaacgagcg aggcgcggct tcgcgaagcg cagcgggtcg cgcgcgtggg gagctgggag
     6841 tgggacatcg agcgggactc gctctggtgg tcggaagagc tgtacgggat ctacggcgtg
     6901 gattccgcct ccttccccgc caccctggag tccttcatcg cgctcgtgca tcccgaggac
     6961 cggcccctgg tccggcgggt gatcgaggcg gcccaccgga accggcagcc gttcaagatc
     7021 gagcaccgca tcgtgcgccc cgatcagacg gtgctcacca tgagcgcgcg cggccgcgtg
     7081 gtcaccgacg agcaggggcg ggcagtgcgg atgctcggca ccggacagga cgtgactgac
     7141 gagaagcggg ccgaggagga gcgctcgcac ctcctggtgg agcgcgaggc acggcgccag
     7201 gcggaggaag cgaaccggct caaggacgag ttcctggcca ccctctccca cgagctgcgg
     7261 acgccgctca acgcgatcgt gggctgggcc aacctgctcc gcgaggggaa cctggatccc
     7321 gccacgacga cccgcgcggt ggagacgatc agccgcaacg cccagatcca gagccgactc
     7381 atctccgaca tcctcgacat ctcccggctc atcgcgggcc agctggactt caagctccag
     7441 atggtgagcc tcgagaacgt gatcgagggg gcgctcgaca ccatgcgccc cgcggcggag
     7501 gcgaacgggg tgcgcctcga ggcgaatctc gctcccggga tcggctcggt gctcggaagc
     7561 cccgaccgcc tgcagcaggt cgtctggaac cttctctcaa acgcgatcaa gttcacgccg
     7621 aaggggggac gcgtctggat cgttctcgcc gcgcgcgagt cgagcgccgt catccgcgtg
     7681 gaggacgagg ggatcggcat cgatcccgac ttcctgccgc acgtcttcga gcgcttccgg
     7741 cagaagaatc cttccagctc gcggcggcac ggcggcctgg ggctggggct cgcgatcgcg
     7801 cggcacatcg tcgaattcca cggcggcacg atcacggcgg agaaccgcga gagcgggcgg
     7861 ggcgcgcggt tcgtcgtgac gatgccgctc gtggaagcga ccgaggcggc ccgtcccggt
     7921 gaaggcgacg ccgcggggcg gagcgccgcg ggcgccgatc gacgccccgc gccgtccggc
     7981 gcgccgcgcg ccgccgcgcc ggccgaaggc gcggaggcgg agcccgcgcc gaacccgctc
     8041 gcgggggtgc gcgcgctcct cgtcgaagac gaggccgact cgagggagct cctggcgcgg
     8101 ctcctcacga gctgcggcgc cgaggtgcgg gtcgcgggca gcgcggccga ggcgctgggg
     8161 cttctggtcc gcgagcaccc caacctcctg atcagcgaca tcgcgatgcc cggtgagacc
     8221 ggctacgacc tgatcgagcg cgttcgcgcg ctccctcccg aggccggcgg ccggatcccc
     8281 gcgatcgcgg tgaccgccta caccggcgcg gaggacgcgc gccgcgcgct ccgctcgggc
     8341 tatcaggccc atctctcgaa accggtccag ctgcgggagc tggtgcgcct ggtggcggat
     8401 ctcagggacg gctcttcgct cccgctcgaa tcccccggac cctcgggacc gcatggccgc
     8461 tgacggaagg gggcgtgtcg cgcggacggg cgagccgctc ggcctgatgg gcgtgttcgc
     8521 ccatcccgac gacgaaacgc tgggcgtggg cggcgtcttc gcgcgctatg ccgccgaagg
     8581 ggtacggacc tccctggtca cggcgacgcg gggcgaccgc ggccgcttcc gcggcattcg
     8641 ccacggggag ggtccggagc atcccggtcc cgaggcgctc gcgctgatcc gcgagcagga
     8701 gctgcgcgcc gcgtcgcgcg tgctcgggat cgaagacgtc acgcttctcg actacggcga
     8761 cggccgcctg gatgaagccg acccgcggga ggcggtggcg cggatcgcgg cggagatccg
     8821 ccgcgtccgg ccgcacgtgg tgctcacgtt cgctccggac ggagcgtacg gacatccgga
     8881 tcacatcgcg atctgccagc tcgccaccgc atcggtcgtc gcggccgcct cgggggacgc
     8941 gccgcacacc gtctccaagc tctactacat ggcgacgacc caggcgcgct gggacgccta
     9001 ccaggcggcg ttcaagaagc tcgtgtcgct ggtggacggc gtggaacgcc aggcgcagcc
     9061 gtggccggag tggatggtca cgaccaccgt ggacacgcgc gagcagtggc ccaccgtgtg
     9121 gcgcgccgtc gtctgccacg actcgcaggt gggaggctac gagaagctga ggcatctcgc
     9181 gccggagcac cacgaagcgc tatggggccg ccagcagttc tatcgtgttc tgagcgtcgt
     9241 gaacggaggc cgcggcatgg aaaccgatct cttcgagggg ttgcgatgac gacgaagacg
     9301 gatggaggcg ccgcgtccgc cggcgcgaat caggcggcgc gacggcaggc gccgctcgcg
     9361 atggatgccg aggcgttccg cgcggcgggg caccgcctgg tggaccgcgt gtccgatctc
     9421 ctcgcctcgg tgccggagcg gccggtgacg cgcggcgagg cgccctccgg cgtgcgggcc
     9481 gcgctcgacc tgaacgggtc cctgccggag gagggttgcg atccggaggc gctgctcacg
     9541 accacggccg ggctcctctt cgaccactcg ctcttcaacg gccatccgcg gttcttcggc
     9601 tacatcacgt cgcccccggc gccggtgggc atcctgggcg agatgctcgc ggcggcggtg
     9661 aacgcgaacg ccggcgcctg gatcctcgcg ccagcagcca cggagatcga gacccagacg
     9721 atccgctgga tcgcggagct gatcggctac cccgcgtcgg cgggcgggat tctcgtgagc
     9781 ggcggcaaca tggcgaactt cgtcggcttc ttcgccgcgc gcgccgccca ggcgccctgg
     9841 cccgttcgcg agaagggcgc cgcgggcgga ccgccgcttc gcgcctacgg ctcggccgag
     9901 acgcacacct ggatccagaa agcggccgac ctggcgggcc tgggcaccga gtcggtgcgg
     9961 tggatcgaga ccgacggcgc gatgcggatg cgcgtggacg cgctgcgccg gaagatccgc
    10021 gaggaccggg agcggggcga cgtgccgttc ctggtggtcg gcaccgccgg gagcgtgggg
    10081 accggcgcgg tggatccgct ccccgagatc gcggcgctct gccgcgagga gaaggtgtgg
    10141 ttccacgtgg acggcgccta cggcggactc gccgccgcgg tgcccgaggc gcccgaggat
    10201 ctccgcgggc tcgctctggc ggactcggtc gccgtcgatc cgcacaagtg gctctacgcg
    10261 ccgatcgagg cggggtgcgc gctggtgcgc gatcccgcgc ggctgcgggc ggcgttcgcg
    10321 taccacccgc cctactaccg cttcggtgaa gagggactga actacgtcga gtacggtcct
    10381 cagaactcgc gcgggttccg cgcgctcaaa gtgtggctgg ccctgcggca cgccggcgcc
    10441 cgcggctacc gcgccatgat cgccgaggac atgcgcctct cgcgcgtgat ggccgagcgg
    10501 gtggacgccg agcccgacct ggagcgcttc acgcagtcct tgagcatcac gacgtttcgt
    10561 ttcctgcccc gcgatctgcg cgagcggcgg ggggaggcgg atgttgcgga gtatctcgac
    10621 cggctgaacc aggacctgct ggaccgcatc cagaacggcg gagagtgctt cgtgtcgaac
    10681 gccgtgatcg gcggccgcta tgtgctgcgc gcctgcatcg tgaacttcca tacgggggaa
    10741 gcggacgtcg cggcggttcc ggtgatcgtc gcgcgggtgg ggcgcgaagt ggacgcggcg
    10801 ctccgtcccg ccgccctccg cgccgcgcgg agctgaaggg cgccgcggcg gccgcgcgct
    10861 agcggaagcg gacgggcagc cgcaccgacg ccgcgtgctc cggcaggtcg gagggattgc
    10921 tccgctcgag gatgagcgtg ccctcctcgg tgtcgggagg ctcgaagtcc agggtgaggg
    10981 agaagggaac gtactcgggc gtcatccagt cgccggtcgc gcgcgccacg ccccgggcca
    11041 cgaccgaatc gcgcgcgtcc agcagccgca ccgggaactc cgcctcgaag aaccacgatc
    11101 cgcgcgcgcg tccggcgatc cagagcgggc tctgggcgat ctggttcggc cggggcgtgt
    11161 cgaggagcac cgggccgagg cgcgtggcgc cgtcctcgcc cggcgaatcg gcggccggct
    11221 cctcgccgcc caccgggatc ccgcccgtca tcaccgcccc cgagtcgatc ccgtagggcg
    11281 gctgccgcgg aggcggagcg gcgaggctct cggcgatgct cgccgcgctg gaatcgacca
    11341 ggtacccggc gtcgggattc gccgcgtcct gatcccgcgg cagcggtcgc agcgcgaagt
    11401 agccgaagcc ggcgccggcc aggagggccg tgaccccggc aatccaccag atccgctgcg
    11461 cgcggctcat ggagccgctc aggccaccaa cacgcggcgc cgcgccgcgg cggggatgcg
    11521 cggctccggc gtgaggatgc cgtccaggtt cagggggtcg gcggccgcga cacgcacgcc
    11581 ggcgccggag ccggcgtcgg agcggcgcag gcggcgcagg agatccaccg cctccggcag
    11641 cgcgtattgc tcgcccgcga agcgctgcac gaagcgtccg ccgcgcacat ctccgcgcag
    11701 ctcccacaga cggtagacgc gcaccagctc ccgccaccgg accggaaggc gttcccggtc
    11761 gagcaggcgg cggaagatca cgccgtagcg atcgatcagg cggcgggcga ggaactcgat
    11821 ctccgcttcc gccgcgagcg tgaagggcgc acccgcggcg agccgccccg cggcaacggg
    11881 gtcatcgccg gcttcccgag gaacgcgcag cacggtccac cgtcccggcg gcgcgaagcg
    11941 agcgcgcttc aaggcgccgc gccggcggct gggcggcgtg atcaggcggc gaagcccgct
    12001 ccaggaatcg cacgtgacga gcccgcggcc gatcagctcg gcgatcccca tttcgacatg
    12061 ggcagggagg aggccggagt gccgctccag gtcctgcgcg aaggaggccc cacgcgccgc
    12121 cagaaccgag aggagaacgt cggcgtaggt cgagatctcg cgcggatcga tctcgccctc
    12181 cgccgtgccg acggcctcgc catcgtcggc gggggcgggc gcccgcgccg cggcgggtgc
    12241 cgcgagcgag agccagaggt ccagctcctc gcgcggcagg agcgcgaccg gcgtcgagcg
    12301 gatcggcgag cgtccgccgc cccagagccg cccccatgcc agctctcccg acagggtcgc
    12361 ctggtccagc cattcctggc ggtaaccctc cacgcgggcg gggaggacgc tcgattccca
    12421 ggccgcggcc gggatctcga acccggcgag ctgtcgcgca acttccacca cgccgcgcgg
    12481 gccctcgact cggtacccct cggccgcgtg ctgccagcag gcgaggaacc gccagaaccc
    12541 ggccgcggtg accggctcga tctcgcgccg cagccgttcc agggtctcgc gatggatgcg
    12601 cgcgaggagc ctccggtcgc accacgcctg gcgcccgtcg agccggcagc ggagcacgcg
    12661 cccttcgacc tcgagcgcca gtagatattc ttccgccgaa ggcggaagaa agccctcgcc
    12721 cgcgcccacg aacacgggcc caagcgcctc gagccgcccg cgcagcaccg tcttcggatc
    12781 cgtcggcgcc tcgaccgcgc gccaacgggc ctcctccccc gccggcgcgt cgcgcagcac
    12841 gcgccgctgc gacgccagct cctcgatcca cggcatccag ccggaaatct ccgcctcccg
    12901 ctccgtcacg taccccatcc agagaagcgc ctcgtgcacc tcctcggcgt tctcggggtc
    12961 gggccatgcc tgctcgcgca cgcgcgccac ggcgtcgggg tcgagcgcgc ccagcgtgtc
    13021 ggcggtctcg ggatccagga agcggcgcga gctgaccgcc tgcgtgcggc gctcttcgag
    13081 cggcgcgtcg tcgaggaagg cgtagggcgc cgcgttcagg atgccgcgcg cgaacgccga
    13141 gggttcgggc gtatccacgg cgacgcgctc gatcgcgccg gagcggagtc cgcgcaagag
    13201 ctcgaggaag ccgtcggcat ccatcgcctc cgtcaagcag tcctcgatgg tctggcgcac
    13261 gatcgggtgc tcccacggca ccggcagctc gccgggagga agcgtctcgg ggcaggcgag
    13321 aacctggggg aacgcctgga tcagcgcgtc ctcggcccgc atgcgcagga gcggcgtcgg
    13381 gaccctcttg ccgccgccct gcgtgcgcga gaggaggagc gcgcgggtca ggttccaacg
    13441 ccatcgcgtg ccgaacatcg gctggtccag gagcgcctgc accaggaggt cgcgcgcggt
    13501 cgccgggtgc aggaactgga agacgtcgtc gagcgggaag ctgtgatgcg gccccagcga
    13561 gatgacgatc gcctcctcgt tggccgcggc ctgcagctcg aagccgaagc cccggcagaa
    13621 gcgtttgcgg agcgcgagcc cccaggcgcg gttgatccgg ccgccgaagg gcgcgtgcac
    13681 gaccagctgc atcccccccg actcgtcgaa gaagcgctcg agcacgaccc gctccggggt
    13741 ggggatcgcg cccagcgacc gctctccttc gcggagatag tcggagagct gcgtcgccgc
    13801 ctcggccgag agcccgaggt cgttcgtgag ccacgcggcg tcgcgcccgc ggacccgcac
    13861 gcgtccgacc gccgccgcca gctcgcgcgt gcgcgccgga gcctccccca gccagaaggg
    13921 gagggtcggc ggagcgccgt gcgcgtcggc cacgcgcacg atgccgggct ccacgcggag
    13981 cacctgccag gaggcgttgc cgagctggaa gacgtccccg ccgttcgact ccacggcgaa
    14041 gtcctcgtcc agggtgccga cgaacgtgcc ctccggctcg accaccacgc ggtactggcc
    14101 ggtgtcggga atcgcgcccc ccgacgtcag cgccgtgatc gaggcgcgcc gggtggcgcg
    14161 gagcttcccg ttcacgccgt cgcgatggag cagcgcggcg cgccccgcgg tgtggaggcg
    14221 cacgacgtcg tcgaattccg cgcgcgccag gtcctggtag ggccacgcgc gccggagcgt
    14281 gtcgtagagc cgctcctcct cccaggtctc gggaacgcag gaggcgacga tctgctgcgc
    14341 caggatgtcg agcgagcggc gcggcatggg cgtgcggtcc agctcgcccg agcggatcgc
    14401 ttcgagcagc gccgccgccg tgaccagctc gtcctgggtc agcgggaaga tgcgcccctt
    14461 ggggacgcgg cccaggccgt gtccgctccg gcccacgcgc tgcaggaaga ccgagatcgc
    14521 gggcgtcacg ccgatctgga tcgcgaggtc cacgtcgccg atgtcgatcc ccagctcgag
    14581 ggaggccgtg gcgaccagcg cgcggagcga gccctgcttc agccgccgct cggcgtccag
    14641 gcgccgctcg cgcgaaaggc tcccgtggtg gctggtcacc tgctcttcgc cgagaacctt
    14701 ggtgaggcgg gccgcgatgc gctcggccag cttccgcgtg ttcacgaaga cgagcgtcgt
    14761 ccgatgctcg ccgatcagct cgcccatccg cttcgtgatg tcgtcccacg tttcatgcga
    14821 gcagaccgtg gcgagcggcg cgtccggaag ctcgatgccc aggtccagcg cacgccggtg
    14881 gccggtgtcc acgatggcgc agtcgcgccc cacgcccacg aggagctccg ccacgtcggt
    14941 gaccggcttc tgcgtcgccg agagcccgat ccgctggatc ggcccaccgg ccagcgcttc
    15001 cagccgctcc agcgagagcg cgagatgggc gccgcgcttc gagcccgcga cggcgtggat
    15061 ctcgtccacg atcacggtac gcaccgtgcg cagcagggaa cgtcccccgg cgctcgtgag
    15121 gaggatgtag agcgattcgg gggtcgtgac cagcacgtgc ggcgggcgcc gtcccatcga
    15181 ggcgcgctcg ctggcggggg tgtcgccggt gcgcaccagg acgcggacgg ccgcgcactc
    15241 ggggtccagg agcgtgagct cggcgagcgg cgcctggagg ttcttctgca cgtcgttgct
    15301 cagcgccttg agcggcgaga cgtagaggac ctgggtttcg tcaggcagcg cgccgccctg
    15361 gcggaggagg ccgtccaggg cccagaggaa cgcggcgagc gtcttgcccg aaccggtcgg
    15421 cgccgcgatc agggtgtgcg cgccggagcg gatccgcggc cacccctcgg cctgcgccgg
    15481 ggaggggacg ccgatccgct cgcggaccca tcgcgcgacc gtgggatgga agccgtcgag
    15541 gggatcgccc aggcgatcgc gatcgcgggc ctcttcgggc gtggccccgt ttcggtcagc
    15601 catggatcag gcccgccacg atcttcaagt ccgcgacgaa gcgcgccatc tcccgccggc
    15661 gctcgtcgga gccgcgctgg cggaggatgg aggaggggtg cacggtggcg accgtccgcg
    15721 gcgcgagcgc tgtcggcacg atgcgcccgc gcatggcggt caccttgaag tcgcgcgcga
    15781 cgatcgcctg agccgcggtc gcccccaggc agacgagcac gtccggacgg atcagctcca
    15841 tctcgcgctc caaccaggga aggcatgccg cgatctctct cgcgctcggc ttcttgtgct
    15901 tccgccgctt cccctgcggc tcccacttga agtgcttgac cacgttcgtg acgtaggtct
    15961 cgtcgcgcgc gatccgggcc gcttcgagcg cttcgtccag cacgcggccg gccggtccca
    16021 cgaagggatg gcccgcgagg tcctcgtcgt tgcccgggac ctcgccgacc aggacgaccc
    16081 gcgccgtgcg cggcccctcg ccaaagacgg tctgcgtcgc gttccggtag agcggacagc
    16141 cgcggcagcc gcgagccgcc tcgcgcagcg aggccatcgt gagacgcgcg ggaaggaact
    16201 cgagggcgct ggcgccggtg gtggaacgcg gtgtcatggg agaattgtac cccggccggc
    16261 ggggctcgag ccatgcggcg gagcgtgccg tgacacgcgg ccgcgtcgag cggccgcgcg
    16321 cccggcccgc ctcacgtgct cttgacttcg aactccttcg tcgcggcaga ggtgccgttc
    16381 acgaagacct cgaccttgta cttcccgacg gggaagccgt ccggcttcga gacgtggaac
    16441 tcggtcaccg cctgcccgct gggggcgatc gtccgggtcg attcgtccac gacctggccg
    16501 tcctggtacg tgaaccgcgt cttgagctcg gccgaagggg ccgacccgtt ggtcacgacc
    16561 gatgcgtaga tcgtctggtt cggcaggaac gtgttcgtct gggtggccac ggtgttgtca
    16621 ttggcgaggc tcgtcccgag gtcgacgcgc gccacctgaa cgccctgggc cgagggagtg
    16681 gtcccctccg tgcccgaagt gctcgaggtc tgggttccgt tcttctggca ggcgccggtg
    16741 aaggccgcaa ccgtcaggca gagcgcgacg gcggcggcac ggtgatcgaa tcgcatacgt
    16801 cctccttcac gtggggtgct agggaagccg cagggtccag cccggctcga tgtggtccgg
    16861 atcgtggatg cggtccttgt tcgcttcgta aatcttgtgc cactggccgg catcgccgta
    16921 gtgatgccgg gcgatcttgg agagcgagtc tccgggcttg acgacgtacg tggctcccgc
    16981 gccgccgtcc tgagcgggcg atggcgcggt cgacgacgat ccgctctgca cgttggagaa
    17041 atcgggccgg gattgtttct tgtcatccgc catggcggca cctcgaatgg ggataggtcg
    17101 acttccgccc ccgcagacgc gggaccgcga aagcctgcat cccccatgcc acccgggcgc
    17161 cggggcgtcc cttcgggaac cgctgccggg cgggggcggc cctgggcacg catgatgcga
    17221 tcgctcccgg gccatgatcc tgatcgtggg cgcccacccg ggcgacgagt cggccggcgc
    17281 ctcgtccgtt ctcttttctc ccgacgacgt ctcggtcctc cacgcgaccg acggcgcgcc
    17341 gcgcgacccc gccgaggcgc gagccaacgg gttcgagacc cgtgaagcct atgcggcggc
    17401 gcgcgaggcc gaggcgcggg cggcgctcgc gttcgcgggg atcccgcctt cgcgccgcgt
    17461 gacgctggga cagatcgggc gggaagcttc ccttcacctc gcggcgctga cgcgggcggt
    17521 cctggcgacg atcgagcggc tgcgccccga agtccttctc acgcacgcct acgagggggg
    17581 gcacccggac cacgacgccg tcgcgttcgc cacgcatgcc gccgtggcgc tgcggcgcgc
    17641 ctccggctcg ccggtcccag ccctctcgga gatggcctcc tatcatcggg gccccggcgg
    17701 aggcttcgag tcgggggtct tcctgggcgg tgccgcgccc gaagacatcg tgcgccggct
    17761 ggacgccgcc gcgcgcgccc gcaagttgca gctcctcgcg cgctatcgaa ctcagtggag
    17821 cttcctgcga cggttccgcg tcgaggagga gcgcttccgt cccgcccctt tctacgattt
    17881 cacgcggccg ccgcacgccg gccgcctctg gtacgagccc tttccctggg gcatgagcgc
    17941 ggcgcggttc ggagatctgg tgcgctacgc gctgctggag ctggggctcc gccaggcggc
    18001 cgagcccgta tccagcacgt cgtgattcgt catcgcgcgg cacccgccgg atcggcgacg
    18061 aggcagcgag tcagctgagc gcgccagcgc tctcgcgcga gctcgagacg cgcgcgctcg
    18121 ggcgcacccc gttccacctc cgcgaagaac gcggtctggc cggcgcggtg cgcggcgaat
    18181 gcggtgcggt aggcgtgaag cgtggcgggc gccggcggcg gtccaccggc atcgccgtag
    18241 ccgcggagca tcgcctcggc ctgcgcccca tgtagatccc attcctccag cgctccggcg
    18301 agatcccaga tcaccggctg cggccccgtc caggtgtggt ccacgtcgtg tccgccgacg
    18361 tcggtcttca cgatggcccc cgaccgggag cggatccact catggggcgc cagatggccg
    18421 tcgccggctc cgggcgcgct tggctcgtcg cggcccttcc gaaactcggc ctcggccgcg
    18481 cggaccgcct cgttgccgag gatctccccg gcgttggcgc gcagcattgc cgccgtccgc
    18541 tcgcgcgctt cgtgggaggc ttcgggggaa agcggcggaa gggccgcccg cgcgatgtag
    18601 gcgcccatcc ggaccaggaa ggggagcgag gcgtcctgga tgccgagagg acggccgtcc
    18661 acccactccc aggccacgaa gccgtgcatc tcgccgagca ggcgcggagc gaacccgagc
    18721 gccgcgaggg gagcggaccg ccgcgcgatc tgagccgcca gcgtgcgctc catccccggg
    18781 gcggtcgcgt gcccgcagaa cttgaagagc acgcggcccc cgtcctgcga ggcgcagaga
    18841 agcttggggc gctcgagcgg gcgcagcacc gcgggccacg cccgctcccc gccgtaatgg
    18901 gctgcgcgcc acatgccgcc gcccacctcg gtgacgcgga cgagatcgcc gaaggccgtt
    18961 cgcactccgg tccagagctc ctcggccagt gtccgctcgc cgaaccgctg cggcgaatcc
    19021 atcgccgggt aggagcggaa ggatcgccag cgggagcgcg cctccgccgg cgcgcctccc
    19081 gggccgttgc catgcgccgg gaagagggcg atatcatcga tgccggtccg ccgcatggcc
    19141 tcggcggccg cgagcatcga ggagccggac aggccgggac cctcgtccac gacgatcccg
    19201 aagggcgccc cgggtagcgg gacttccaga cggcgctcga ggggctggcc cgacgggcgc
    19261 acggtgatcc gcttggcgcg gaatccacgc gccctcagcg tggccatgac gaccgccgac
    19321 agcgtcgtcc cgatggttcg gatcccaacc acgagaacgc gccggtcggg agcgttctcg
    19381 tgctcgagcg cccagcggag cgcctggtcc cgataggact gcggcatgag gccatagaac
    19441 gcgaaaccct cgggcacctt cacgtcgacg gtgccgcagc gcctgcgcag cggggcgagc
    19501 gcgcggcagg aggcgccgac cgcccgcgcc gcctccccgg gcgtacccga gaaggagcgg
    19561 tcgaaggccg tcgcggccag ggtcgtgaca cggcgagcgg cggccaggtc gcgcgatcga
    19621 gcgcgtagcg ttccgacctc ctcggagtca tcgaagaggg cctgctcgag ctcgcccgcg
    19681 cgctcgacca gtccgcgcac gcgcgccggc cggaggtccg cggtttgggc ctgctcgagc
    19741 atgaggttca gcgtttcgac gcagcgctcg agcggctccc ggcgcgaggg atctccgtag
    19801 acgatcatcg cgcgacttcc ggcgccgccg gcgcggcgct tccgttgagc gaagccgggt
    19861 tctcgagtgt ctcgaaaacc ggaggcgcgt cgttcagacg gaccaaggtg gctccgtccg
    19921 cggccagaac ggcgccgtcg ctgtggcgct cgcgctggcg ctcgagcgcg gccaccgccc
    19981 gcacctgcgt ctggagcatc gtctcgccgc cgggaacggg ggtcacgctg cggcacgtgt
    20041 tgaacgcccg ccaggcggga accgcctcga gctcttcgaa ggagcgcccg gtccactcgc
    20101 cgaaggacat ctcgcccagc gcgggttcga tctcgggcgc cagcccaagc gccgtcgcga
    20161 tcgggccggc ggtctcgagg ctcctttcga gcggactcgt gacgagggcg tccacgggca
    20221 ccgactggaa tcgctcgcgg agccagtcgg cttcgcggcg gccgcgcgcg ttcaggtgca
    20281 ccccgggcat gcggcccgcg agaacgcggc cgagcaggtc gtgcgaggca tggcgaacga
    20341 ggtagaagcg ggtcatccgg cggggtgcag catcggacgt gccccgatcg aggcaccccg
    20401 taagtcataa cttcacaagc acataggttt tgggagggcg ggtcgcccgc gggcaattct
    20461 acaaggtcgt gaaaagcgtt ctcattgaac cgcccagccc tgccgaccgt ctatactagt
    20521 acggcgccac cgcgccgtcc cgcgtcctaa atcccgattt tcgaggtggt tccatggctc
    20581 ttcgcagacg atcgttccat gcactttatc tctcgttgct cctggccgcc gtggctgttt
    20641 tcggcttcgt cgctgccggc cgggccaaca gcggcttgcc ggcatcgtcc aggatgcttg
    20701 catccctgcc tggaaccgac cccaccgacg cggctcagcc cgcggttatg gggccggccg
    20761 agacgcgaac cgttcccgca tcctggctcg gccattcggg ccggctccgc gcggtcatcg
    20821 acacgccggt ctcgttggag tcgcagctga tcgccgattc gatccaggcc gcgaacctga
    20881 cgcccggact gcacgatctc ggcatcgtca cgccggacgg cgacgccttc tacgtggtgt
    20941 cgctgctgcc ctacgcaacc aaggaaggga agcgcctgaa cggctattac gtcggggact
    21001 ggccgcaggg ggccccgacg gggcgctacg cgccgcccgc cgggttcatc gaggtgacgc
    21061 ccgagaacga agcgacgccc gtctcgcaga gcttcaagct tcaagacttc ctcacccacg
    21121 accagcaggg tgtgtggccc aaggtcctgg tgctgaagcc gaagctggtc gacaagctcg
    21181 agctgatttc ggcggctctg gcggagcgcg gactgcccag ccatctccac gtgatgtcgg
    21241 gattccggac gccgcagtac aacgagcagg gtgtggggcc caagggcggc cgcgccagct
    21301 tgagccggca catgtacggc gacgcctcgg acgtgttcgt ggatgagaac ggcgacggga
    21361 tgatggacga cctgaatgga gacggaagga cgacggtcgc ggacgcccgg gttctcttcc
    21421 aggtggccga gagcgtcgaa gcggcccacc ccgacctgat cgggggactc tcgccctatc
    21481 cttccaacgg cgtccacgga cccttcgtgc acgtggacac gcgaggcttc aaggcgcgct
    21541 ggtagtcttc aagcgcgttc ctctccggcc gtacgacttc ccggcgcggg ggctcaggcc
    21601 ctcgcgccgc tcagttccgg aagcgcttcc tcgatcacgg cagcggtgag ccgcgtccag
    21661 tcgggcgcgc ccacgccgcc gttcttcgtt ttctcgtcgg cggccaggcg cagcagggcc
    21721 gaggacgtgt aggcggccag gcggttcggc cggagcacgc cgccgccgag atatccggcg
    21781 cgcagcgcct cggcccgggc cggcgccatc ttcgtccctc cgcctcgctt cagatccgag
    21841 agcatcatgt gcgcgatcag cgtgcccagg tcgagctccg gctccgcgaa cgacgcgtcg
    21901 tcgaggtcca ccaccgagat cccgtcggag ccgaagatga cgtgcgtggg gcgaagctgc
    21961 cggtgaacca tcgaggggct gcgcgacacg cccccatcgt cggccagctc ggccttcagc
    22021 acgccggctc cgtcgcgcag ccggtcagcg gcgcgcgcgt cgatcccgga ctgccggagc
    22081 cgcgtcacga cgtcgtccag gttctgcgct tccgacgccg catcccatcg cccgaacgaa
    22141 gggcgctcgg tgctgcgggc cgagggtccc ggcgaacgga tcgcgtgaaa cgaagccagg
    22201 gcgcggccga gccgcttcag gctggcggcg acgcggctct cgatcggatc ttcgagcacg
    22261 cccgcgagcg gacgcccgtc catggtggtc tggagcagaa gcttgtgctc gcgcgcgtat
    22321 cccagcggcg tgggaacccg gatctcgggc gcggcggcgg cgcggaggaa acggaggacc
    22381 ggaagcatcc ggtcgccctc gccggagcga tactgcaggc catgccacgt gacgtcggag
    22441 aagggagagc ccgcgcgccg cgcgctcacg cggaagcgga gcgtccggcg gttgtcgggc
    22501 cgctcgctca acacgtcgac cgaacggacc tcctgaacgg tccagccgga gtcggaaatc
    22561 gcgttctgga gaatccgcgc cgcgcggctc ggatcgagga tgggatctcc gtgtctcgtg
    22621 tccatcggag gcgacgccgg ctcgcgcgaa gcgaaaccgc tgctgctgct gatgtggctc
    22681 atggtggctc cgatctgtcg agcccgggtc gcgggcgtcg gtcttggtgc gagaagagcg
    22741 aatgggactc ggaagagtgt cacagcaatg cccgtgccat gatgtgtggg tcccccatcc
    22801 ttcgcattgg cctacgcttc cgccgcgccc gcatcgtttc gcgcgatcgc ggctcctccg
    22861 ccttttctac aactctccac gcgaactgtc cccgatcgaa cactcgcgcg atgcgtctcg
    22921 tcatgaacca ggcatttcga attcatcagg agatccgtcg ggagaatcag gcagaggtgg
    22981 cggaggcaag gattctagag ggagtcgctg ccgcgcggac cggacccgcg gggcgtcccg
    23041 tcgcgacccg gacagtcgcc ccggcgcccg cgggccggcc gcgcgaacgc gacgcttctc
    23101 accgaagcat cgcgtattcg ggcatctcgc gttccagctc gtcgacgcgc gccgcgcgca
    23161 cgcgcgccgc gatccgtccc gcgccgtagg ccacggcggt ggcgccgagg actccggcga
    23221 ggagttggag cccggcgggc atcgccggga ccggatcgaa gacgcgatgg cgtcgggaac
    23281 cacggaactc ggagacctcg tcttcggcat cgtgcggctc tagccggttc agcacctcgc
    23341 gcacgccgcg aacggaggag accgcgccga tcagcgattc cgcctctccg cgcaggatcg
    23401 gtccgttcag gaccaccgat ccgtctcgcg cctccacgga caccgccccc gggtgcgtga
    23461 cgtaccggca cagcttggcg cgcacgcgct cgacgatcgc gtcatccggg cccgatggcg
    23521 cggccgcggc ggctcgcgcg acgagaccgc cggcccggtt cccccgattc tgaagatcgt
    23581 gcaccgcgtc atcgaggaat gcgcggctgt ccccgagcgc acccgtgatc tggtcgcgaa
    23641 ttcgcgcgcg gcgcgtccgg ccccgcgagg gatcgaggag gtacatgagc cccgcgccca
    23701 gcacgaggcc gcgatagaag tttggtgacc ggaccatgga tgctccctcc cgtggcgcac
    23761 aaccgcgcta tacttcggcg cagcaacagg catgccgcgt cttccgactc gaggagtcgc
    23821 ggggcggcgc ctcggcttac acccgccgcg gtttcaccac ggcgcgtgct ttcttgtaac
    23881 attggcctgc aactttcggc ccgcagcgag ggcgtcgcat cggcgcggag gtcccgcttg
    23941 attcgaatga cggtgaagga gcgaagctga gccggtccga cgcgcggagc ttcttcgacg
    24001 gccggacctt cgacgacttc ctcttccggc ccggcaaagg gcgcgtcggc tcgcggcgcg
    24061 aggtgcgcct cacgggtcgt ttcacgggcg ccatcgcgct cgagctgccc atcgtctccg
    24121 ccaacatgga cagcgtcacc gagggcccga tggcgcgcgc gctcgcgctg gagggaggca
    24181 tcggtgtcgt gcatcgcgcc ctcccgatcg gagagcaggc ggccgaaatc gagcgcgtca
    24241 agcggagcca cggctgggtg gtcgaagagc cgctctgcct tcctcgaggc gcgagccttc
    24301 gggaagcgcg cgacgtgatc cggcggcacg gcatcaccgg gatcctcatc gaagaggagc
    24361 gggggagccg cgcgctcgcg ggactcctct ccaaccgcga catcccgtgg gggcccgggc
    24421 acgaggccga gccggtcgag cgcttcatga ccccgttcga ccggctggtc actggcgcgc
    24481 cgggcatcgc gctcgaagag gcggagcgcg tgctctacga gcggcggatc gagaagctcc
    24541 cgctggtgga cgcgacgcgg aggatccggg gcctgatcac gaagagcgac atccagctcg
    24601 cgcgcgagcg ccccgagtcc accaaggacg ccaaggggcg tctccgcgtc gcgggcgcga
    24661 tcggagcccg gggcgatttc ctggagcggg cggaggcgct gctcgacgcg ggcgcggacg
    24721 ccctggtcgt ggacatcgcg cacggccatt ccgaggtgat ggagcaggcg gtgcaggccc
    24781 ttcgggcgcg gttcggcagc gtccccgagc tggtctgcgg caacgtaggc acggcggagg
    24841 gcgcgcgctt cctgcgggac ctgggcgcgg cggccgtgaa ggtcgggatc gggcctgggc
    24901 gcggctgccg cacgaggctc gagacggcgg cgggggttcc gcagctccag gcgatccatg
    24961 aagcgcggat ggccctgggc gagggaacgc cgatcatcgc cgacggcggc ataaggcacg
    25021 acaaggatct cttcctcgcg ctcgtctgcg gcgcggacgc ggtcatgctg ggcagcatgc
    25081 tctcgggaac cgacgaagcg cccggacgcg taattgagga cccatcgagc cgagaaaagc
    25141 gtaaaatata ccgtggcatg acatcccccc aggcggtctt ccaggcgctg tacgacgaca
    25201 ccgatccggt cgagctggaa caggcgctcg acactccatc ggaaggacag gaaatccagg
    25261 tgccgtaccg cggaggcgtc cgcgacgtgc ttcatcgcgt ccgcggccac ctgcgctccg
    25321 cggtgagcta cgcgggagag gaatcgctcg cctgcgttcg cgagcgtgtc ctcgccaacc
    25381 cctcggacaa tctgatcgcc ctctccgcgg cgtcccgccg cgaatccttc gaccgctagc
    25441 gaccgctggc aggcaggctc actccttgcg acttacatcc atgggtggcc gggcgcgcgt
    25501 gcccgcgctc ccgcctccag ccggcgacgg cagggaacgg gcaggtaggc tgcacgactc
    25561 caatgtcttc ccgttcacga atcagcgccg ccggggatcg cctcggagcg tacagccgtg
    25621 tcccgcgcgg ggcggttgga acagattaca ggggaccacg gtagagtctt ccatcgcagg
    25681 gaaggaggcg acgatggctc agatcgatcg ccgcagcgag acggagcagg aagaacagcg
    25741 acggctggac gaggaacggc gtgtccgtca ggccgaaacg gggatgggcc cgcgccgcga
    25801 gaccagcgag gaatatcgcg agcgccgccg tcgcatggag agggatcagg gagcctggcc
    25861 gtacgcgggg ccgttgtctg caaccggcta ccgaccgccg ggctatatgg gatatcccgg
    25921 ctacccgagc tatccctgga gccgtttctc ccacgggatg gatccctctt acgaagactg
    25981 gcaggggccc ggcttcggca cgcacacgcc gggcggctgg gagccgcgcg tcgagacctt
    26041 ccgccgcggg gacgacctga tcgtccgtgt ggagctgccg gggtgggaca aggacgaggt
    26101 gagcgtgttc gtcgaggagg aagacctgat cgtggaagga gcgcgcgagg agggcgccga
    26161 aggagacccg gtccgtcggg acgaggagcg ccgcaaggat gagcggcgag gcgagtggag
    26221 cggcagccgc tacgcggccg agcgcttctc cacgcgggtt tcgctgccgg cgggcgccga
    26281 tcctggcgac ctcaaggcga agttcaagaa cggggttctg gaagtgaaga ttccgatgcc
    26341 ggaacgacgc accgagcgtc ggacggtcaa gatcgacacc tgagagacgg gagagccggc
    26401 gccgcatcca agctaaggga tccatgaaaa tcgacctcaa tcgttttcgc gcgccgctcc
    26461 gcttccaggc aggggtgttt ctcgaccgca agctcttccg cggcatgccc gcgcccaagg
    26521 cagcctccga gccggaccac ggccggaggc tgccggctcg gctctgggcc gccgcgggca
    26581 tcggtctcat cctctttgcc cactgggaga tgtcgacggc ggccttggag tcccgcctct
    26641 tctcgtcgct cgcttcccgg atgacctacc gcgtggagcc gggcaagagc ccacggatca
    26701 ccttcccgaa gtcgggcccg ttcgacacgc ggctcggcta ctcgcgcgtg cccgagttcg
    26761 cgaagcggct cgagtcgcgc ggcttcgaat accagtccca ggcccgcttc acgaagacca
    26821 tggcgctggt cgccgatctc ggagtgccgc ccccctatcc cgagcccccg gtcgccgggc
    26881 tggtgatcga cggctcgggc ggcgagcccc tctacgacgc catgggaacc cggcgcacgt
    26941 tcgcgcgctt cgagcagatc ccgccggacg tcgtgaagag cctccttttc atcgaaaacc
    27001 gcgagctgga ccgcttcgac agcccgtacg ccaatccggc catcgactgg agccgctcgg
    27061 ccaaggcgat ggtcatctac gcggccagcc gcctcggcct ccccacgccg gtcgagggcg
    27121 ggagcacgct ggccgtccag atggagaagt accgccattc gccgagcggc cggacctccg
    27181 gggccgtgga gaagctgcgc cagatgatcg gcgcgagcct gcgcgtctat cgccagggac
    27241 cgcggacggt cgaggagcgc cggcgcatcg tgctggacta cgtgaacacg gttccgctgg
    27301 gcggaacgcc ccgctatggc gaggtcttcg gcatggacga gggactgcgg gtctggttcg
    27361 gcaccgagcc ggagaaggtt tacgccgcgc tggccaagcc gcgcgtcacc aaggcgcgcg
    27421 cgcgggcgct caaggaagtg ctcgcgctgg tctgcgcggt gcgcgcgccc tcccggtatc
    27481 tcgtttcgga ccggggggct ctcgaggacc gggtggacaa gttcgtgaag ctgatggcca
    27541 gccgggggct cctcgagccc aagctcgccg acgcggtggt caagacgcgg ctccgcttcg
    27601 ccgacgtgtc gacccagcgc taccgcaccc agaccgacaa agcgacggtg ctcgtgcgca
    27661 tgcgcctccg gaacctgctc ggcgtcccga atctatacga cctcgaccgg ctgcacgtga
    27721 acgtgaagac caccgtgaac gcggggctgg aacgcgacgt caccgccttc ttccaccgga
    27781 tgaacgacga ggggtacgtg gactcgacgg gactcaaggc ggcgcacctc ctcctggacg
    27841 gcgatccgga ccgcgtcgtg tacagcatga tcctccggga gcgcacgccc acgggagacg
    27901 tggtgcgcgt gcacgccgat aacgtggccg ggcccttcga cgtgaacgag gggacgaaga
    27961 tggagctggg gagcaccgcg aagctccgga ccctggtcca ttacctgaac gtgatgtcgg
    28021 atctgcatca gacgctctcg gcgctcccgg acgacgtggt tcgggatcgg gcgaaggcgt
    28081 cccgcgaccc gctgacgcag tgggcggccg agaccgtccg cgacaacccg ggcatcaccc
    28141 tgtcggactt cctctcgaag tcgctcgacc ggaagtattc ggccagcccc tacgagctgt
    28201 tcttcacggg cggcggactg ctcacgttcg agaactacga caagtcggag aacagccaga
    28261 tcctgccgat ccgcgaagcg gtcgtgcatt cgaccaacct cgtcttcatc cggctcatgc
    28321 gcgatctggt ccgctaccac gaggcgcggt tgccgtacga cgcggacaag attctcggac
    28381 ccaaggacac gcccgagcgc acgtggatgc tccgccaggt cgcggccgaa gagggaacgc
    28441 cggagcagat ggcttggctc ttccgcacgc gcaaccggtc ggcccaggac aaccgccttc
    28501 gcgcgcggat cgagcgcgac gccttcgcgc gcatgacccc ctactggcgg cggctcggtt
    28561 tccccttctc gcacctcgtt ccttcgtacg cgacggcgat cggcagctcc tcggaccagc
    28621 cggcggcgct ggccgatctg atgggcgttc tggtgaacga cggcgtgcgc cgtccacccg
    28681 cgttgatcca ggagatcgcg tttgcgagcg acacgccgta cgagacgcgg ctggatgccc
    28741 ccgcgccgca gggcggcgac ctgctggtgc cgcccgaggt ggcgcgggcc gcccgctcgg
    28801 tcctgggcgc ggtggtcgaa cggggcacgg cggcgcgcct gcacggcgcc ttcgtcgact
    28861 cgaccggggc ccccatctgg gtcggcggca aaacgggtac gggagacaac cgcttcgaca
    28921 ccttcgggcg gggtagaagg ctcatctcct cgcgggccgt gagccggacg gcggcgttcg
    28981 ccttctgcct cggcgaccgc tactacggcg tagtcacggc ttcggtcctg ggccccgagg
    29041 ccgaccagta cacctttacg agcgttctgc cggtcgaagt gctgcggcgg ctcgctcccg
    29101 tcatcgagaa gaggctgcgc ctggtggggg gtaccgctcc ccccggcgcg accgtggccg
    29161 cggtccagga tggcgctgcg gagtccgacg acgtgtcgac cgactccacg gccgtccccg
    29221 cgtctccggc caacgtcggc gccgctcccg cacccgacac cgctcccgcc gctcccgcgg
    29281 acatcaagtc cggaacgccg gccaagacag gggaagcgga cgacgcgcgg aagatccatg
    29341 acgacgcgat gaagaatcgc gatgcccagc gcgcgaagga ccggcgccag aacgagcgcc
    29401 gtcgccaatg ggattgggaa tcgccgtcgc gcacttccgc cggagggcgg ctctagctcc
    29461 ggccgccgcg ggcgatgtgc gcgatcagct ccgtcaggaa gggcgcctgg ccgggatgga
    29521 tgcggcgcgc cgcctcctcc ggagcgaacc aggcggcccg gtccacctcc ggaaaggtcc
    29581 gctgccgtcc cgaccgcggc ggccactcca gcgtgaacgt attgctccgt accgccgagg
    29641 cgtccaggtc gccctcgacc gcccaggcgt ggatcacctt gccgctcttc tgccgtagcg
    29701 ggcggagcgg cgtgaaggga cccgccgcgg cggagccggt ctcctcttcg aattcacggc
    29761 gcgcggcgtc gagcgcgggc tcgtcgtcct cgaattctcc cttcggaatc gaccacgcgc
    29821 cccaatcctt gtccttccag aacggtcccc ccggatgcac caacaacact tcgagcgagc
    29881 cgccgcggtg acgaaagagc aggagcccgg cgctcgttct attcgacatg ggagggttgc
    29941 atcatggcca cgatcacctg gctcaccaac gtggacgaag ccctggcgaa agcccgggcg
    30001 ggaaacaagc cgcttcctcc tggattttag cgcggccccg atgcgctcgg cttccatcgg
    30061 ttccacgcca catggacacc cacgcagctg gtgctggagc ccgacggaac ggagcgctcg
    30121 ctgcgcgagc gccatcccgc gagcgagtgg acccggaagg catccgtctg ggccggttag
    30181 ccgatccggt gtagactgcc ggcgtgaccc cccagcccga ccggatggca gccgtcgagc
    30241 gcgccgtcga gagccgcctg gagcggaatc tcgaggagct cgcgcggctc gtcgcgcagc
    30301 cgagcatcgc ggcgcagaac gtcggcatgg acgcgtgcgc cgagctggcg gccggaatgc
    30361 tgcgcgagcg ggggttctcg gtcgagtttc cgcgcgtgcc gggctttccg gtgatcgtgg
    30421 cggaacgcca cggcgcctcc gaccgtacac tcctcatcta caaccactac gacgtccagc
    30481 ctcccgagcc gctcgagctg tggaccagcc ctcccttcga ggcgacgcgg cggggcgacc
    30541 gcctgatcgc gcgcggcgtg agcgacgaca aggggcattt cacggcgcgc ctgctcgcga
    30601 tcgacgcgct gctcgccgag cggggcgacc ttccgtgccg gatcctctgg gttctcgaag
    30661 gggaagagga gatcggaagc cccaacctcc cggcgttcgt ccgcgaccac aaggagcgcc
    30721 tcgcggggga cgcctgcctc tgggagttcg gctacgtgga cccgggcggc gtgccggtgc
    30781 tgcacgcggg actccgcgga atctgctacg tcgagctgtc ggtcgagacc gcggcccgag
    30841 acgcccattc gggcgtcggc ggctccatct tcccaaacgc ggcgtggcgg ctcacctggg
    30901 cgctggccag tctcaagggc cctgacgagc ggattcgggt cccgggccac tacgactcgg
    30961 tgcggccgcc ctcatcgcgc gaccgcgagc tgatggaggc catgcccgag acggcggggg
    31021 agtatcgcga gctctacggc gtgacgcggt tcttgagagg gctcgagggg ggcgccgagc
    31081 tccgcatcgc cgaggtgttc gagccgacct gcacgatctg cggcctggac tccggctatc
    31141 agggagcggg ctccaagacg gtccttcccg cccgggcgtc ggccaaggtc gacttccggc
    31201 tggtacccga ccagacgccg gcggaggtgc ttcagaatct acgcgcgcac ctcgaccgcg
    31261 agggattccc ggacgtgacg atacgcttcc tgggcggcaa tcccgcgggc cgcaccgatc
    31321 cggatcatcc cttcctgcgc ctggtggccg agacggccat tcccgcgttc ggcgttccga
    31381 tgcggatcgt gccgatgatc ggcggctccg gtcccaacga gtcgttcatc cgatatctcg
    31441 gcgtaccggt cgccacggcc ggcttcggct accccgacac gagggcccac gctccggacg
    31501 agaacctcct cgtgtcgttg tacgagaaac acgcgaagca cgccgcccgg ctcatcgacg
    31561 cgttcgcgag cggctcctga gcttctccta gagccggccg ctcgtggagc gctcggtcgc
    31621 gcgcgtgcgc ggcgagctcc ccgag
//
LOCUS       DASSLZ010000247         4006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_599288, whole genome shotgun sequence.
ACCESSION   DASSLZ010000247 DASSLZ010000000
VERSION     DASSLZ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599288"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFM00_09390"
     CDS             <1..325
                     /locus_tag="VFM00_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498503.1"
                     /translation="ANPKVTVVNGNLAINGTWTGGGILMVNGNLAMGGGCQYKGIVVC
                     TGDLTMTGGGPADVARIIGAVIYQGTLVNASSFSGSGRIWYSSEAINSALNVNRYTLA
                     WWRER"
     gene            358..1686
                     /locus_tag="VFM00_09395"
     CDS             358..1686
                     /locus_tag="VFM00_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498504.1"
                     /translation="MNRIVASKRGLPNAEAGNALIIALLILFLLTSLGVSYVAVTKGD
                     KQVAGNQLTSTMAFSNAEAGISEALARMSNPNASGTTYIGENPSFGYTAGWGRYIITD
                     PGNSGLDPQYNATTADGLDNNGNAAIDESSEHYPERSSKQNSGMALSDKLDYPWVKVR
                     YKLNGANQIVLFGDHDNNPTTPPQENLVRGIPKLIITSAGRRGTGQKIVTVEAVKWPL
                     PPVPGSVYTEGPMNFNGAAFYIDGHDHDYTAPYDTVSGATPLPGIATTNAPSTISSQL
                     NGQQSDNVVGSGGDPSVAPASVNLDLQAMATAWGDMADITLNGDQTNPSTSGWGSVAT
                     SPPTLKIVHIAGGDLHISGNTSGAGVLVVDGDFVLSGAFNWNGVVLVLGDVTVTGGGS
                     AKQIVGALMVQGTVSGTSALNGNIKCLYSSAMISQLNALSRYEISSWIDQ"
     gene            complement(1726..3852)
                     /locus_tag="VFM00_09400"
     CDS             complement(1726..3852)
                     /locus_tag="VFM00_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498505.1"
                     /translation="MSRLSPRAVRALLALLLVGIAAFHVGRVLRSERAITGGEICLPL
                     DDSFIYLQYARAIAEGHPFVYTPGNAPTTGATSLAWPILLAPPHLMRLDAALCIAWAL
                     GLGIVGYALSALLLARIGTRLGGVAGGGLAVLLFVMSPFLLWGYLSGMEIALYATVLL
                     ASFLAYLREREAARFPSLRWWLFALAVARPEGAVLAGVFGLLMIADRGRASRRAGAGG
                     PGLGWSALLPFAAAALPFVITLLISGSFEGTSAQAKSVLAEPYRETRLEYLRGTPNVW
                     LSIGKVYLGMMQLEGGLRLPAALTLVNGVGVALFLILAFVPRRRPWASGLALLPLLAA
                     GIVIQSLPVFWYVHLYRYLQGVYPLFLLLAAAGWGRLATLAWDRLPRAAGAPVAALLA
                     IAPLVVWAPRLLPEQGRVIEFYGHNCENILHQQVAIGRWIDRSLPPGAIVGLNDAGAI
                     AYYGRRHTVDLIGLTSADYARVYRSGLGCLFEHVRREPPSRRPTYFAIYPGWFPYWRE
                     SGILGPESFRAHLGFNTICGGPDMMVYPASWVDAAATDRPLDSDSTLAGKRIVDSLDL
                     AWLDDERRHEWRADPEAKDVLRLYSFADRPTRPVADGGRIVYGSQRFRAAVAPGRDLT
                     LILRTDAWYPSRVRVRVDGKDAGRWSIARSDTTWVEPSFRIPGALLTRPRPEFALERE
                     GAAGAPDVGLGSEAGNLAPFHIWLAQ"
BASE COUNT          739 a         1329 c         1414 g          524 t
ORIGIN      
        1 cgccaatccc aaggtgaccg tcgtgaatgg caacctggcg atcaacggaa cctggacggg
       61 cggcggcatt ctcatggtga acggcaatct cgccatgggt ggcggctgtc agtacaaggg
      121 catcgtggtc tgcaccggcg atctcacgat gaccggcggt ggtcccgcgg acgtggcccg
      181 catcatcggc gcggtgatct accagggaac cctggtgaac gcatccagct tcagcggctc
      241 gggacgcatc tggtattcga gcgaggcgat caattcggcg ctcaacgtga accgatacac
      301 gctcgcctgg tggcgcgagc gctaggccag ggacggcgga cggaagggga tcggaccatg
      361 aaccgcatcg tcgcgtcgaa acggggtctc ccgaacgcgg aggccggcaa cgcgctgatc
      421 atcgcgctcc tcatcctgtt tctcctcact tcgctcggcg tctcctacgt cgcggtgacg
      481 aagggggaca agcaggtggc gggaaatcag ctcacgtcga cgatggcgtt ctcgaacgcc
      541 gaggcgggaa tctcggaggc gctcgcgcga atgtccaatc cgaatgcgtc cggcacgacg
      601 tacatcgggg agaacccatc gttcggttat accgcgggct ggggacgata catcatcacg
      661 gacccgggca attccggact cgatccccag tacaacgcga ccacggccga tggcctcgac
      721 aacaatggta acgcggcgat cgacgagtcg agcgagcact atccggaacg cagttcgaaa
      781 cagaattccg gaatggccct atccgacaaa ctcgactatc cctgggtcaa ggtgcgctac
      841 aagctgaacg gagcgaatca gatcgtgctg ttcggcgacc acgacaacaa tcccacgacc
      901 ccgccccagg agaacctggt gcgcggcatc cccaagctca tcatcacaag cgcgggacgg
      961 cgcggaacgg ggcagaagat cgtgacggtg gaagccgtga agtggccgct gccgccagtg
     1021 ccgggctctg tgtacacgga ggggcctatg aacttcaacg gcgccgcgtt ctacatcgat
     1081 gggcacgatc acgattacac ggccccgtac gacaccgttt ccggagccac gcccttgccc
     1141 ggaatcgcga cgaccaacgc gccgtcgacc atctcctccc aactgaacgg acagcagtcg
     1201 gacaacgtcg tcggctcggg aggggatccg agtgtcgcgc ccgcgagcgt caacctggac
     1261 cttcaggcca tggcgacggc gtggggcgat atggccgaca tcacgctgaa tggcgaccag
     1321 accaatccca gcacgtccgg atggggaagc gtggcgacat cgcctccgac cctcaagatc
     1381 gtccacatcg ccgggggcga tctccatatc tcgggcaaca cgagcggcgc cggcgtgctc
     1441 gtcgtcgacg gcgacttcgt cctgagcggc gcgttcaact ggaacggcgt ggtgctggta
     1501 ctgggcgacg tgacggtgac gggcggcggt tccgcgaagc agatcgtggg agccctgatg
     1561 gttcagggca cggtttccgg gacgtcggcg ctgaacggta atatcaagtg tctctactcg
     1621 agcgcgatga tcagccagtt gaatgcgctc agccgttacg aaatctcgtc ctggatcgac
     1681 cagtagtcgc ctgaagctcg ctggcgcgcc gctgaggcgt ccgggctact gagccagcca
     1741 gatgtgaaac ggagcgaggt ttcccgcctc gctccccagc cccacatcgg gagcccccgc
     1801 ggcgccttcg cgttccaggg cgaattcggg acgcggacgg gtgagcaagg ctcccgggat
     1861 cctgaacgac ggttcgaccc acgtcgtgtc cgagcgcgcg atggaccacc gccccgcatc
     1921 ctttccgtcc acccgcacgc gaacccggct cggataccac gcgtcggtgc gcaggatcag
     1981 cgtgaggtcg cggcctggtg ccaccgcggc gcggaagcgc tgcgagccgt agacgatccg
     2041 gccgccgtcg gccaccgggc gggtcggacg gtcggcgaac gagtagaggc ggagcacgtc
     2101 cttcgcctcg ggatccgccc gccactcgtg gcgccgctcg tcgtcgagcc acgcgaggtc
     2161 cagggaatcg acgatccgct tacccgccag cgtggagtcc gagtccagcg gccggtcggt
     2221 ggccgcggcg tccacccacg aggcggggta gaccatcatg tcggggccgc cgcagatggt
     2281 gttgaagccg aggtgcgcgc ggaaggactc ggggccgagg atgccgctct cccgccagta
     2341 ggggaaccag cccgggtaga tcgcgaagta ggtcggacgt cgcgacggcg gctcccgccg
     2401 cacgtgctcg aacaggcagc cgagcccgga gcggtagacg cgcgcgtagt ccgccgaggt
     2461 cagcccgatc aggtccacgg tatggcgccg gccgtagtag gcgatcgccc ccgcgtcgtt
     2521 cagccccacg atcgcgccgg gcggcaggct tcgatcgatc cagcggccga tcgccacctg
     2581 ctgatggagg atgttctcgc agttgtgccc gtagaactcg atgacgcgcc cctgctcggg
     2641 gagaaggcgc ggcgcccaga cgacgagcgg cgcgatcgcg aggagcgctg cgaccggagc
     2701 gccggcggcg cgcggcagcc ggtcccacgc gagggtggcg agccgccccc acccggccgc
     2761 cgccaggagg aggaagaggg gatagacccc ctggagatac cggtagaggt ggacgtacca
     2821 gaaaacggga agggactgga tcacgatccc cgcggcgagc aggggaagaa gggccagccc
     2881 cgaggcccag ggccggcggc gcggaacgaa cgcgaggatc aggaagagag cgaccccgac
     2941 tccgttcacg agcgtgagcg cggcggggag gcgcagacct ccctcgagct gcatcatgcc
     3001 caggtagacc ttcccgatcg agagccagac gttcggcgtg ccgcgcaggt actcgaggcg
     3061 cgtctcgcga tagggctccg ccaggaccga cttggcctgg gcgctcgtcc cctcgaacga
     3121 gccggagatg aggagcgtga tgacgaacgg caacgcggcg gcggcgaagg ggaggagcgc
     3181 gctccagccc agccccggcc cgccggcccc ggcgcgccgc gaggcgcgac cccggtcggc
     3241 gatcatcagg agcccgaaga cgcccgcgag cacggcgccc tccggacgcg cgacggcgag
     3301 cgcgaagagc caccagcgca gcgatgggaa ccgcgccgcc tcgcgctccc gaaggtaggc
     3361 gaggaacgag gcgaggagga ccgtggcgta gagcgcgatc tccataccgc tcagatagcc
     3421 ccagaggagg aacggactca tcacgaagag gagcacggcg agcccgccgc cggcgacgcc
     3481 gcccagccgc gtgccgatcc gcgcgaggag cagcgcgctc agcgcgtagc cgacgatccc
     3541 cagccccagc gcccacgcga tgcagagggc cgcgtccagg cgcatgaggt gcggcggagc
     3601 gaggaggatc ggccacgcga ggctggtcgc ccccgtcgtc ggcgcgtttc ccggcgtgta
     3661 gacgaaggga tgcccctcgg cgatcgcgcg cgcgtactgg aggtagatga agctgtcgtc
     3721 gagcgggagg cagatctcgc cgccggtgat cgcgcgctcc gatcggagga cccgccccac
     3781 gtgaaacgcc gcgatcccga cgagaaggag ggcgaggagc gcgcgaacgg cgcgcgggga
     3841 gagacggctc atcgcgtcct tcccggccgc gccacggggg gagcgtgcgg cgccttcggg
     3901 cccggcgacg cctcgctcga atccacccac gcggccaggg ccaccttgta cagaggatcc
     3961 ggaggatatc cggccggctg gagcgtgaag atcgagagca gcgccg
//
LOCUS       DASSLZ010000248         9454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_472756, whole genome shotgun sequence.
ACCESSION   DASSLZ010000248 DASSLZ010000000
VERSION     DASSLZ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9454
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472756"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1077
                     /locus_tag="VFM00_09405"
     CDS             67..1077
                     /locus_tag="VFM00_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7498506.1"
                     /translation="MRAVTLRRPGPPTSLRVEEAPEPHAGPGEVLIQVQAAGVNFTDV
                     LSRQGLNPEAPPRPYVLGHEASGDVIALGEGVDRFRVGQRVLSYHMTGGYAERIAVPA
                     EQVIAIPDSLAYQCAATLPLNYGTAYVALYRTGPVERGMRIFIHAAAGGVGMAAVDLA
                     RRAGLEMTGAAGTHFKRARLLSEGVKHVVQSRHLHIARLGQRIYGGRAYDIVLDSVGG
                     PSIRDGLKSLRPGGRVVSLGVSGLSGKGVLGALSYLLRAPRFTFIDLLRPSLGLHGVN
                     LRELIRNQALMRRVLEELVSLADLGEITPQPGRVMGLAEAGEAHRLLQSRANVGKIVL
                     RM"
     gene            complement(1078..1929)
                     /locus_tag="VFM00_09410"
     CDS             complement(1078..1929)
                     /locus_tag="VFM00_09410"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7498507.1"
                     /translation="MPPTDVAVFRSFRDPVLAVWPYWSRPELLDRWLGTTDVELEAGG
                     SFQATLWNGDVVRATILSAAPPSRLDLAWRGEGPDAAQTVRVRLEHQGPGCRVAVEEE
                     DPGSDLERAHACAWWHAALDALRAAVGGIPDAHRWGDALPIVLRAPLGREASDIWPLL
                     STASGLAKWLASAERFDPAPGGAFRFVSRFKGSEIVEEGRVEALEPERLVALSWEWLG
                     QGWQAPTRVELRLESDPTGTALVIHHSGFEALEPEQRLAARVNYAAAWRDVMDDLKRL
                     VAPGPTG"
     gene            2115..2783
                     /gene="lexA"
                     /locus_tag="VFM00_09415"
     CDS             2115..2783
                     /gene="lexA"
                     /locus_tag="VFM00_09415"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET7498508.1"
                     /translation="MLTPRQRATYDFVIRFSRRNGYAPSYDEIRRHLGLASLNGVAKL
                     IAQLRRRGYLVAAPPNAKRWLAAAAPGREWAADRTAPPLETRVRRSTRPEAATLPLLG
                     TIAAGRPIEAIENPEAIEVPASLLGAGERYALRVRGDSMVDDGIQDGDVVVVRSARRA
                     ENGQTVVAIVDGEATLKRFHGPGRGGRWVELRPANAAMKPLRVAADRLEIRGVLVGLL
                     RRYT"
     gene            2802..4070
                     /locus_tag="VFM00_09420"
     CDS             2802..4070
                     /locus_tag="VFM00_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498509.1"
                     /translation="MERILVHLAIDSFPIQAERLRCPKLMGRPVALTPPDSSRPRVLT
                     ASREAAAHGVYPGMPLLAARRVCRDLVPIPPDADLYAGVSDSIRDRLLPLAPLGGAPR
                     PGRFVLDLTGLARTHGDACDRASRAGQEVERWFGLHPTLGVASTRLVSAVAAGVLAPD
                     GELLDVPSGSEAAFLGPLAVRVLPSARGAFASERLDLLAVRLVRDAQALALRQLEAAV
                     GRATAFSLWREARGLDGAPERAGLPPTAVAEETLAAETNDRRVLGARMQRLALEVGFG
                     IRRRGTAANRLEVGAIYADGRHGRARRVIREGISGERALGAAALALLDRALTRRVRVR
                     RIRLAAWEESASAEQLVLWPALGGNRPSGGSEDSASMGEVVAPAGGASRRAADFDAAL
                     DRLRRRLGTDALVPASWMALGVALRPSARP"
     gene            4039..7041
                     /locus_tag="VFM00_09425"
     CDS             4039..7041
                     /locus_tag="VFM00_09425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET7498510.1"
                     /translation="MSRFVHLHVHSHYSMMRGPEPIEALITAARAHGMDRFALTDTNA
                     LYGFVFYRQMCAETGLTPIAGAEILEKEASHAAPGGRGRAVLLARGRSGYASLCHVIT
                     ARHLDPEFSIERSVRSHAADLVLLSEDRKTLESLRDSIQVYAELVPGRGDRPLRAWAR
                     ARGIPCVATNDVHFTRPESRRLHQILRAIDRNTTLEQVPPDDTAEIGRVFASAAAMEA
                     RLAHAPEALENAARLGEECAVDWEMGRTVFPSYPLDKGEAFAILRARCEAGIVHRYGK
                     GPAPGVRARLDRELAIIREKGFSDYFLIVEAITSRTPRICGRGSGAASIVAYLLGITH
                     VDPVRHNLFFERFLSPERKDPPDIDVDFAWDERDQIQLDVLAENGAPVRAAMVANHVG
                     FQQRGAIHEIAKVYGLPEVEIMNVTRRLQGHWGQEDPIDGMQRRPRFRDVDFEPPWPE
                     ILAQAQALEGHPRHLAVHSGGIVLVPDELSNHVPVEIAPKGVPIVQWEKDQAEDFGLV
                     KMDLLGNRSLAVIRDAVAAVERNTGLKIDERTWNPIDDPATQRLMAEGDTIGVFYAES
                     PSMRQLQRKAARGDFDHLVIHSSMIRPAANSYINEYLRRLHGGAYEPLHPALRHTLDE
                     TYGIMCYQEDVTKVSMELAGLPLAQAEQIRKALGHKRPVKPLRAHMADFYAGAASRGV
                     AREVIDKVWDMILSFAGYSFCKPHSASYAMVSFRSGWLRAHYPAEFIAAVISNEGGYY
                     DAFAYVSEARRMGLRVLPVDVNASRREYTGRARDVRVGLMQVKGLRAEATQALLEARE
                     RGGAFASFDNLRRRTRLHVADLERLVKSGACDSIAGGRTRAELLWEVYLDDAASVAAT
                     GTDDLFAPAAPTVPRAEPYDRPTMLRHEIETLGFLVSAHPLEPYERAMRGRGITAGKD
                     LDRHVGRRVKLLGWHVTSKLVHDKHERLMEFVGFEDTTALYDATFFPKVYERVCHLLA
                     GNQPFLITGQVQQEYGVTSVVVEDLRRL"
     gene            7125..7808
                     /locus_tag="VFM00_09430"
     CDS             7125..7808
                     /locus_tag="VFM00_09430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET7498511.1"
                     /translation="MPTETPTQSSWSAVDTYLSGVLLPDDPALEEAVRSSERAGLPAI
                     QVSPLQGKMLHLLALMRGARSILEIGTLGGYSTIWMAKALRPGGRLITLEIDPKHAEV
                     ARANLLRAGLERVVDVRVGRALDTLERLAAENAGPFDLVFIDADKPSGAEYFKRTLGL
                     SRTGTIIVVDNVVRDGEVADASSSDANVVGSRRVLEAMAAEPRVSATAIQTVGVKGYD
                     GFAMALVVS"
     gene            7805..8845
                     /locus_tag="VFM00_09435"
     CDS             7805..8845
                     /locus_tag="VFM00_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7498512.1"
                     /translation="MTRSAEPASGRAAAGAAAEANPFESLRQIYSGYCLARGLHVVAD
                     LGVADALGDTPRSAAELAGSVGADPDALDRLLRLLSAHGIFQSEKGRYHHSAASRLLR
                     SDHPQSARPLARMFGLSLNWSAYGAMEHSIRTGRPAVEMTLPGGFWSHFAAHPEDSAV
                     FNAAMAAKAIAQVAAIVPAYDFSKCERIGDIGGGRGHLLQAVLRSAPRARGILFDLPH
                     VIADASDIASDRLALQGGDFFKDPLPECDTYLVMEIIHDWPDAESAAILGAIRRSARP
                     GATLLLLEEIIPDDPGPHWSKVLDIHMLTLLGGRQRTLPEYEHLLREAGFALRREIET
                     AAGISILEAEAV"
     gene            complement(8893..>9454)
                     /locus_tag="VFM00_09440"
     CDS             complement(8893..>9454)
                     /locus_tag="VFM00_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HET7498513.1"
                     /translation="RVTDIGPSITWVRTLGAEAVADGIRLEWFLAARNATDLTIERQD
                     RMGWIPIGTVRVTFGTVGFTDTQVRPGSTYRYRLSVHVAGMASSFGEMAVTVPLPSLQ
                     VRGAPANPVRGELAAEVQLPHTAPAILELFDARGRLIERQEIAGGAPRVERFRLGAGR
                     RLASGVYFFRVSQEGQSAAKRVVFIR"
BASE COUNT         1340 a         3386 c         3336 g         1392 t
ORIGIN      
        1 cacgcgcgat gtggcaggag gtgttcgaca cggcgtcgag gctgcgcgat cgggtatagt
       61 gccgcgatgc gcgccgtcac ccttcgaaga cccggtcctc cgacatcgct ccgcgtggag
      121 gaagctcccg agccgcatgc cggccccggc gaggtcctga tccaggtcca ggccgcgggc
      181 gtcaacttca ccgacgtgct atcgcggcag ggattgaatc ccgaggcgcc gccccgcccc
      241 tacgttctgg ggcatgaggc cagcggcgac gtgatcgcgc tcggcgaagg ggtggatcgg
      301 ttccgggtcg gccagcgggt gctttcctac catatgaccg gcggctacgc cgagcggatc
      361 gccgtccccg ccgagcaggt gatcgcgatc cccgattccc tcgcctatca gtgcgcggcg
      421 acgctcccgc tgaactacgg cacggcgtac gtggcgctct atcgcaccgg acccgtcgag
      481 cgggggatgc gcatcttcat ccacgccgcg gctgggggcg tgggcatggc ggcggtggac
      541 ctcgcgcgcc gcgcgggtct ggagatgacg ggcgcggcgg ggacccactt caagcgcgcg
      601 cggctcctgt ccgagggggt gaagcacgtg gtgcagtcgc ggcacctgca catcgcgcgc
      661 ctggggcagc ggatctatgg cggccgcgcc tacgacatcg tgctcgactc cgtcggaggt
      721 ccgtccatcc gcgacgggct gaagtcgctt cgccccggcg gacgggtggt gtcgctgggg
      781 gtgagcggcc tctcggggaa gggcgtgctg ggcgcgctga gctacctgct ccgcgcgccg
      841 cggttcacgt tcatcgatct gctccggccc agcctgggcc tccacggcgt gaacctgcgc
      901 gagctgatcc ggaaccaggc gctcatgcgg agggtgctgg aagagctggt ttcgctggcg
      961 gaccttggcg agatcacgcc gcagccgggt cgcgtgatgg ggctggccga agcgggcgag
     1021 gcgcaccggc tgctgcagtc gagggcgaac gtggggaaga tcgtgctgcg gatgtagtca
     1081 gcccgtcggg ccgggtgcca ccagccgttt caggtcatcc atgacgtccc gccacgccgc
     1141 cgcgtagttc acgcgcgccg ccaggcgctg ctcgggctcg agggcctcga agcccgagtg
     1201 atggatcacg agcgcggtcc cggtcggatc ggactccagc cgcagctcca cgcgggtcgg
     1261 cgcctgccac ccctgcccca gccactccca cgagagcgcg accagccgct ccggctcgag
     1321 cgcctccacg cgcccctcct cgacgatctc gctccccttg aaacgcgaca cgaagcggaa
     1381 ggccccgccc ggcgcgggat cgaagcgctc cgcgctcgcg agccacttgg cgagacccga
     1441 tgccgtggag agaagcggcc agatgtcgga tgcctcgcgt cccagggggg cgcgaagcac
     1501 gatcggcaac gcgtcgcccc agcgatgggc gtcggggatc ccgcccaccg cggcccgcag
     1561 cgcgtccagc gcggcatgcc accacgcaca ggcgtgggcg cgttccagat cgctcccggg
     1621 gtcctcttcc tcgaccgcga cccggcaccc cggcccctgg tgctccaggc gaacgcggac
     1681 cgtctgcgcg gcgtccggtc cctcgccgcg ccaggcgaga tccaaccgac tgggcggagc
     1741 cgcggacagg atcgtcgcgc gcaccacgtc gccattccac agcgtggcct gaaacgagcc
     1801 gcccgcctcc aactcgacgt ccgtcgtccc gagccatcgg tccaacagct ccgggcgcga
     1861 ccaatacggc cacacggcga gcaccgggtc ccggaaggac cggaacacgg ccacgtctgt
     1921 cgggggcatg cgggcatcct agcacgcttg actttcacct tttattcacc tatcttcatt
     1981 catgtccctt cgggcgtctt cctaccgcct gggggagcgg gtcccggacg gctgctgaca
     2041 gggcgccacg cggccgcctc gggagcccgc agggaactgc ccgcctcgcg cgggctccac
     2101 gacgagagga agggatgctc acgccacggc agcgcgcgac atacgacttc gtgatccgct
     2161 tctcgcggcg gaacggctat gcgccgtcct acgacgagat ccggaggcac ctgggcctgg
     2221 cctcgctcaa cggggtggcc aagctcatcg cgcagctccg gcggcggggc tatctcgtcg
     2281 ccgcgccgcc gaacgcgaag cgctggctcg cggcggcggc tccggggcgg gaatgggcgg
     2341 cggacaggac ggcgccgcct ctcgagacgc gcgtcaggcg ctcgacccgc cccgaagccg
     2401 ccacgctccc gctcctcggg acgatcgcgg ccgggcggcc gatcgaggcc atcgaaaacc
     2461 ccgaggcgat cgaggtcccc gcctcgctgc tcggcgcggg ggagcgctac gcgctccggg
     2521 tccggggcga ctccatggtg gacgacggca tccaggacgg cgacgtggtg gtcgtgcgga
     2581 gcgcgcggcg cgccgagaac ggacagaccg tcgtcgccat cgtggacggc gaggcgacgc
     2641 tcaagcggtt ccatgggccg gggcgcggcg ggcgctgggt ggagctgcgg ccggctaatg
     2701 ccgccatgaa acccctgcgc gtcgccgccg accgcttgga gattcgcggc gtgctcgtcg
     2761 gacttctgcg ccgctacacc tgatcctcca cgagccgtgt catggagcgg atcctcgtcc
     2821 atctcgccat cgactccttc cccatccagg ccgagcggct gcgctgtccc aagctcatgg
     2881 gacgcccggt cgcgctcacc ccgcccgact cgtcgcggcc gcgcgtgctc accgcctcgc
     2941 gcgaggcggc cgcgcacggg gtctacccgg gcatgccgct cctggccgcg cggcgcgtct
     3001 gtcgcgacct ggtgccgatc ccgcccgacg ccgacctcta cgcgggcgtc tccgattcca
     3061 tccgtgaccg cctcctgccg ctggcgccgc tcggcggcgc gccgaggccg ggacgcttcg
     3121 tcctcgacct gacgggcctg gcgcgcacgc acggcgacgc gtgcgaccgc gcgtcgcggg
     3181 cgggccagga agtcgagcgg tggttcgggc tccatcccac gctgggtgtc gcctcgacgc
     3241 ggctcgtgag cgcggtggcg gcgggcgtgc tcgcgcccga cggcgagctg ctcgacgttc
     3301 cctcggggag cgaggccgcc ttcctcggtc ccttggcggt gcgggttctc ccctccgcac
     3361 gcggggcgtt cgcgtcggag cggctcgatc tcctcgcggt gcggctcgtg cgcgacgccc
     3421 aggcgctcgc gctgcgccag ctcgaggcgg cggtcgggcg cgccaccgcc ttctcgctgt
     3481 ggcgcgaggc gcgcggcctc gacggcgcgc cggagcgcgc cggcctgccg cccaccgcgg
     3541 tggccgagga gacgctcgcg gccgagacga atgaccgccg cgtgctcggg gcgcgcatgc
     3601 agcggctcgc cttggaagtg gggtttggga tccgccggcg cggcacggcc gcgaaccgtc
     3661 tggaggtcgg ggcgatctac gctgacggcc ggcacggccg cgcccggcgg gtgattcgag
     3721 aggggatttc gggagagcgc gccctcggcg ccgccgcgct cgcgctgctc gaccgcgccc
     3781 tcacgcggcg cgtgcgcgtg cggcgcatcc ggctcgcggc gtgggaggaa tccgcaagcg
     3841 ccgagcagct ggtgctgtgg ccggccctgg gtggcaatcg accttccggc gggtcggaag
     3901 actcggccag catgggagag gtcgtggctc cggccggcgg cgcttcgcgt cgcgcggccg
     3961 acttcgatgc cgcgctcgat cggctccgcc gccgactggg cacggacgcg ctcgtgcccg
     4021 cttcatggat ggccctcggt gtcgcgcttc gtccatctgc acgtccatag ccactactcg
     4081 atgatgcgcg ggccggagcc gatcgaggct ctgatcaccg ccgcacgcgc gcacggcatg
     4141 gaccgcttcg cgctgaccga cacgaacgcg ctgtatgggt tcgtgttcta tcggcagatg
     4201 tgcgccgaga cggggctcac gccgatcgcc ggcgccgaga tcctcgagaa ggaggcttcg
     4261 cacgccgcgc cgggggggcg cggccgggcg gtgctgctcg cgcgaggccg atcgggctac
     4321 gcgtcgctct gccatgtgat cacggcgcgg catctggatc ccgagttctc gatcgagcgt
     4381 tcggtccgca gccatgcggc cgatctcgtg cttctctccg aggaccgcaa gaccctcgag
     4441 tcgcttcgcg actcgataca ggtgtacgcc gagctggttc cgggacgcgg cgaccgaccg
     4501 ctacgcgcct gggcgcgcgc gcggggtatt ccctgcgtgg ccacgaacga cgtgcacttc
     4561 acgcgccccg agtcgcgccg actgcaccag atcctgcgcg ccatcgaccg caacacgacg
     4621 ctcgagcagg taccgcccga cgacacggcc gagatcggcc gcgtgttcgc ctcggccgcc
     4681 gccatggagg cgcggctcgc ccacgcgccc gaggcgctgg agaatgccgc ccgcctcgga
     4741 gaggagtgcg ccgtggactg ggagatgggg cgcaccgtct ttccgtcgta cccgctcgac
     4801 aaaggggagg cgttcgcgat cctgcgcgcc cgctgcgagg cggggatcgt gcaccgctac
     4861 ggcaagggcc cggcgcccgg ggtgcgcgcg cggctggacc gggagctcgc gatcatccgc
     4921 gagaagggct tctccgatta tttcctcatc gtggaggcga tcacgtcgcg cacgccgcgc
     4981 atctgcggcc gcgggtcggg ggcggcgagc atcgtggcct acctgctcgg catcacccac
     5041 gtcgatccgg tgcggcacaa cctgttcttc gagcgcttcc tctcgcccga gcgcaaggac
     5101 ccgcccgaca tcgacgtgga cttcgcctgg gacgagcgcg accagatcca gctcgacgtg
     5161 ctcgcggaaa acggcgcccc cgtgcgcgcg gcgatggtcg cgaaccacgt cggcttccag
     5221 cagcgcggcg cgatccacga gatcgccaag gtctacggcc tgcccgaggt cgagatcatg
     5281 aacgtgaccc gccgcctgca ggggcactgg gggcaggagg acccgatcga cggaatgcag
     5341 cggcggcccc gctttcgcga cgtggacttc gagcccccgt ggcccgaaat cctggcgcag
     5401 gcacaggcgc tggaagggca cccccggcac ctggccgtac acagcggcgg gatcgtgctc
     5461 gtccccgacg agctgtcgaa ccacgtgccg gtcgagatcg cgcccaaggg ggtccccatc
     5521 gtgcagtggg agaaggacca ggccgaggac ttcggcctgg tgaagatgga cctcctgggg
     5581 aaccggtcgc tggccgtcat ccgcgacgcc gtggccgcgg tggagcggaa cacgggcctg
     5641 aagatcgacg aacggacctg gaatccgatc gacgatcccg ctacgcagcg gctcatggcc
     5701 gagggggaca cgatcggcgt cttctacgcc gagtccccct cgatgcgcca gctccagcgg
     5761 aaggcggcgc gcggcgactt cgatcatctc gtgatccact cctcgatgat ccgccccgcc
     5821 gcgaactcgt atatcaacga gtacctccgc cgcctgcacg gcggcgccta cgagccgctc
     5881 catcccgcgt tgcgccacac gctcgacgag acctacggga tcatgtgcta ccaggaggac
     5941 gtgacgaagg tctccatgga gctggccggg cttccgctcg cgcaggcgga gcagatccgg
     6001 aaagcgttgg gccacaagcg ccccgtgaaa ccgttgcggg cgcacatggc cgacttctac
     6061 gccggcgccg cgtcccgcgg cgtcgcgcgc gaggtgatcg acaaggtctg ggacatgatc
     6121 ctctccttcg cggggtattc cttctgcaag ccgcacagcg cctcctacgc gatggtctcc
     6181 ttccgctcgg gatggctccg cgcgcactac cccgccgagt tcatcgcggc cgtgatctcg
     6241 aacgaggggg gctactacga cgccttcgcc tacgtgtccg aggcgcggcg catgggactc
     6301 cgcgtgctgc cggtggacgt gaacgcgagc cggcgggagt acaccggccg cgcgcgcgac
     6361 gtgcgcgtgg ggctgatgca ggtgaagggg ctgcgcgccg aggcgaccca ggcactgctc
     6421 gaggcgcgcg agcggggggg cgcgttcgcc tcgttcgaca atctccgccg ccgcacgcgg
     6481 ctgcacgtgg cggatctgga gcggctggtc aagtcgggcg cgtgcgactc gatcgcgggg
     6541 ggccgcacgc gcgcggagct cctgtgggag gtctacctgg acgacgccgc ttcggtcgcg
     6601 gccaccggca ccgacgatct cttcgcgccg gcggctccca ccgtgccgcg cgcggagccc
     6661 tatgatcgac ccacgatgct ccgccacgag atcgagacgc tcggcttcct cgtgagcgcg
     6721 catccgctgg agccctacga gcgggccatg cgggggcgcg gcatcacggc cggcaaggac
     6781 ctcgaccgcc acgtcggccg ccgcgtgaag ctcctgggat ggcacgtcac gtccaagctg
     6841 gtccacgaca agcacgagcg gctcatggag ttcgtcggct tcgaggacac gaccgcgctc
     6901 tacgacgcca ccttcttccc caaggtgtat gagcgggtct gccacctgct cgccgggaat
     6961 cagccgttcc tgatcacggg ccaggtgcag caggaatacg gagtcacgtc ggtggtggtg
     7021 gaggatttgc ggcggctctg acgggtcgaa cgcctctgtt ggcggaacgc cgtttccgcg
     7081 ctattcttac gccctcaaaa caggcgttca ggggggagac tgccatgccg accgagacac
     7141 cgacgcaatc atcctggtcc gccgtcgaca cctatctcag cggagtcctc ctgccggacg
     7201 atccggcgct cgaggaagcc gtccgctcca gcgagcgcgc gggccttccg gccattcagg
     7261 tctcgccgct ccaggggaag atgctccatc tgctcgcgct gatgcggggc gcgcggtcga
     7321 tcctcgagat cggcacgctc ggcggctaca gcacgatctg gatggccaag gcgctccgtc
     7381 ccggaggccg cctgatcacg ctggagatcg atccgaagca cgccgaagtc gcgcgcgcga
     7441 atcttctgcg cgccggactc gagcgcgtgg tggacgtgcg tgtcggccgc gcgctcgaca
     7501 ccctggaacg gctcgcggcg gagaacgcgg gtccgttcga tctcgtcttc atcgacgcgg
     7561 acaagccgag cggcgccgag tacttcaagc gcacgctcgg gctctcgcgc accggcacca
     7621 tcatcgtcgt ggacaacgtg gtccgggacg gcgaggtggc ggacgcgtcc agctccgacg
     7681 cgaacgtggt cggctcgcgg cgcgtgctcg aggcgatggc cgccgaaccg cgcgtcagcg
     7741 ccacggcgat ccagacggtc ggcgtgaagg gctacgacgg cttcgcgatg gcgctggtcg
     7801 tttcgtgacc cggtcggccg agccggcatc ggggcgagcc gctgcgggcg ccgccgctga
     7861 agccaatccc ttcgagtcgc tccgccagat ctacagcggg tactgcctgg cgcgcggcct
     7921 gcacgtcgtc gcggacctgg gggtcgccga cgctctgggc gatacgcccc gctcggcggc
     7981 cgagctggcc ggatcggtcg gcgcggatcc ggacgccctc gaccgcctcc tccgactcct
     8041 ctcggcgcac gggatcttcc agtccgagaa gggccgatac catcactccg cggcgtcccg
     8101 gctcctgcgc tccgatcacc cgcaatcggc gaggccgttg gcgcgcatgt tcggactctc
     8161 gctgaactgg tcggcgtatg gggccatgga gcactcgatc cgaaccggaa gaccggccgt
     8221 ggagatgacg ctgccgggag ggttctggag ccatttcgcg gcgcatcccg aggacagcgc
     8281 ggtcttcaac gcggccatgg ccgcgaaggc gatcgcccaa gtggccgcga tcgtccccgc
     8341 ctacgatttc tcgaagtgcg agcgcatcgg agacatcggc ggcggccggg gacaccttct
     8401 ccaagccgtg ctgcggtcgg ccccgcgcgc cagggggatt ctcttcgacc tgccccacgt
     8461 gatcgcggac gcgtccgaca tcgcatcgga ccggctggcg ctgcaaggcg gcgatttctt
     8521 caaggatccg ctgcccgagt gcgacacgta tctcgtcatg gagatcattc atgactggcc
     8581 cgacgccgaa tccgccgcca tcctgggtgc gatacggcgc tccgcgcgcc cgggtgcgac
     8641 gctcctcctc ctcgaggaga tcattccgga cgacccgggc ccgcactggt ccaaggtgct
     8701 ggatatccac atgctcacgc tcctcggagg gcgccagcgc acgctcccgg agtatgaaca
     8761 tcttcttcga gaggcaggat tcgcgctgcg ccgcgagatc gaaacggcgg ccggtatttc
     8821 aatcctcgag gcggaagcgg tctagcgcgg caagcagcac gatgcccgag agttcttttg
     8881 cgggtttttt cgttaccgga tgaagacgac ccgcttcgcg gcgctttgcc cctcctggct
     8941 cacccggaag aagtacaccc ccgacgcgag acggcgcccg gcgcccagcc ggaatcgctc
     9001 gacacgcggc gcgccccccg caatctcctg ccgctcgatg agccgcccgc gcgcgtcgaa
     9061 gagctcgaga atcgcgggcg ccgtgtgcgg cagctgcacc tcggccgcga gctcgccgcg
     9121 cacggggttc gctggcgccc ctcgcacctg cagcgagggg agcggaaccg ttacggccat
     9181 ctcgccgaag ctcgaggcca tgcccgcgac gtgcaccgag aggcgatagc ggtaggtcga
     9241 gcccgggcgg acctgagtgt ccgtgaatcc gacggtcccg aacgtcacgc gaaccgttcc
     9301 gatcgggatc caacccatgc ggtcctggcg ttcgatcgtg agatcggtgg cgttccgcgc
     9361 ggccaggaac cattccagcc ggatgccgtc cgcgaccgcc tcggcgccga gagtccgaac
     9421 ccatgtgatc gacggcccga tgtcggtgac gcga
//
LOCUS       DASSLZ010000249         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1611804, whole genome shotgun sequence.
ACCESSION   DASSLZ010000249 DASSLZ010000000
VERSION     DASSLZ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611804"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFM00_09445"
     CDS             complement(<1..828)
                     /locus_tag="VFM00_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7498514.1"
                     /translation="MKALLTIARKDLKLLTRNRASFFWVLGFPVLVALLFGAAFSGGG
                     GRPSLPVAVVDLDRTDYSRALVRRLHQSEALRVRETTLDSALTAVRHGDLTAYIALRP
                     GMSENFGFGGDSTSGIEIGIDPRQRPTADMLRGLVTQAVFMGMRQSFTTGGAGREMIA
                     QQLEKLAADTTAATDRRLRGQRVLRSLESFMTVLDSMETPGRTAADSAGPPDSASIAA
                     GLGDGAGSSPSDSSSSAMAGPRIHMVEVTEQDNGPRTAYEVTFPSGLAWALIGTCMAF
                     "
     gene            complement(890..1783)
                     /locus_tag="VFM00_09450"
     CDS             complement(890..1783)
                     /locus_tag="VFM00_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010148407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7498515.1"
                     /translation="MSAIRVENLRKSYGDLLAVDGVTFDVRQGETFGLLGPNGAGKTT
                     TIGMIAGTLRPDGGSVQIDGQDDPTRSSVRRRIGVAPQALALYGDLTGDENLAFFGRL
                     YGLSGTHLHERVEWALAFSGLADRRKDRVAKYSGGMQRRLNLVCGLLHDPPVVVMDEP
                     TVGVDPQSRNLIFENIDELKLQGRTILYTTHYMEEAERLCDRVAIMDHGKILALDTVN
                     ALLRQHGRLSTVEIEFADRPRSLEGLGGAWDKGTWRVQTEEPVQVLQRASAAGHLANV
                     SIRRANLEHVFLQLTGRSLRD"
BASE COUNT          333 a          697 c          742 g          322 t
ORIGIN      
        1 gaaggccatg caggtcccga tcagcgccca ggccagcccc gaggggaagg tcacttcgta
       61 cgcggtgcgc gggccgttgt cctgctcggt cacctcgacc atgtggatgc gcgggccggc
      121 catggcgctg ctcgaggaat cggacgggct cgagcccgcc ccgtcgccga ggccggccgc
      181 gatcgacgcg ctgtccgggg gccccgcgct gtcggcggcg gtccgccccg gagtctccat
      241 cgagtcgagc acggtcatga acgattcgag cgagcggagc acgcgctggc cgcggagccg
      301 gcggtcggtc gcggcggtcg tgtcggccgc gagcttctcg agctgttggg cgatcatctc
      361 ccgccccgcc ccgcccgtcg tgaacgattg gcgcatcccc atgaagaccg cctgcgtcac
      421 gaggccgcgg agcatgtcgg cggtgggacg ctggcgcgga tcgatgccga tctcgatccc
      481 ggaggtgctg tcgccgccga agccgaagtt ctcgctcatg cccgggcgga gcgcgatgta
      541 ggcggtcagg tcgccgtggc gcacggcggt gagcgcggag tcgagcgtgg tctctcgcac
      601 gcggagcgcc tcggattggt ggaggcgtcg caccagcgcg cgggagtagt cggtgcggtc
      661 taagtccacg acggcgacgg gcaggctggg ccgcccgccg ccgccggaga aggcggcccc
      721 gaagagcagg gccaccagta cgggaaagcc cagcacccag aagaacgaag cccggttccg
      781 ggtgaggagc ttcagatctt tacgggcgat cgtgaggaga gccttcaccg gcggctcacc
      841 cctatgaccc gatcacgtcc accaccccac gcaccacacc cggtgaccct caatccctca
      901 acgatcgccc tgtcaactgc aagaacacat gctccaggtt cgcgcggcgg atcgagacgt
      961 tggccagatg tcccgcggcg ctcgcgcgct ggagcacctg caccggctct tccgtctgca
     1021 cgcgccacgt ccccttgtcc cacgcgcccc ccagaccctc cagcgacctg ggccggtcgg
     1081 cgaactcgat ctccaccgtg gagaggcgcc cgtgctgccg caggagcgcg ttcacggtgt
     1141 cgagggccag gatcttgccg tgatccatga tcgcgacgcg gtcgcagagc cgctcggcct
     1201 cttccatgta gtgggtggtg tagaggatgg tacgtccctg gagcttcaat tcgtcgatgt
     1261 tctcgaaaat gaggttccgc gactgcgggt ccacgcccac ggtcggctcg tccatgacga
     1321 ccaccggggg atcgtggagc aggccgcaga cgaggttcag gcggcgctgc atgccgccgc
     1381 tgtacttagc gacccggtcc ttccggcggt cggcgagacc ggagaaagcg agcgcccact
     1441 ccacgcgctc gtgcaggtgc gtgcccgaga gtccgtagag ccgtccgaag aaggcgaggt
     1501 tctcgtcgcc ggtgaggtcg ccgtagaggg ccagcgcctg gggcgcgacg ccgatccggc
     1561 gccgcacgga ggaacgggtg gggtcgtcct ggccatcgat ctgaaccgaa ccgccgtcgg
     1621 ggcgcagagt tcccgcgatc atcccgatgg tggtggtctt gccggcgccg ttggggccga
     1681 ggaggccgaa ggtttcgccc tggcggacgt cgaaggtgac gccatcgacg gcgagcaggt
     1741 cgccgtacga tttccgcaag ttctccacgc ggatggcgct cacgaaatcc ccccggcaag
     1801 gtcatggcgc gctcgatacg tctgtgcgcg cgaagtattg cgcagacgcg cgcgcggagc
     1861 caaatcaaat tacgggcgcg tcgcacgcga aaaaaaatcc tgttgcatcg cgagaagtgc
     1921 gatggcgacg cgacgatcag gcgcccgtta ccccgaggta gttgtcacgg tagtaggcga
     1981 cgcggaagcc caggcgcgtc acgttgtgga aggaaggcgc gggcttgtcg ggtttgtcct
     2041 cggcggtctc cacggagaga tgccggcagc ccattgtccg ggcgtcctcg atcc
//
LOCUS       DASSLZ010000250         2902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_93023, whole genome shotgun sequence.
ACCESSION   DASSLZ010000250 DASSLZ010000000
VERSION     DASSLZ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93023"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..213
                     /locus_tag="VFM00_09455"
     tRNA            124..213
                     /locus_tag="VFM00_09455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:158..160,aa:Ser,seq:gga)
     gene            251..326
                     /locus_tag="VFM00_09460"
     tRNA            251..326
                     /locus_tag="VFM00_09460"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:284..286,aa:Ala,seq:cgc)
     gene            381..569
                     /gene="ffs"
                     /locus_tag="VFM00_09465"
     ncRNA           381..569
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFM00_09465"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            596..2503
                     /gene="dnaX"
                     /locus_tag="VFM00_09470"
     CDS             596..2503
                     /gene="dnaX"
                     /locus_tag="VFM00_09470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET7498516.1"
                     /translation="MTHLALARKYRPQRFADLVGQDPVRTTLERAVATNRVAHAYLFA
                     GPRGSGKTTTARLVAKALNCEHRKDGESEPCNQCRSCVEITSGTSLDVLEIDGASNRG
                     IEEIRNLRENVKYAPSGGKSKVYIIDEAHQLTDFAWNALLKTLEEPPPHVRFVFATTE
                     PLDVPDTIASRCQVFEFRRLRSEELVAHLETIAAAEKVKLDPEAAALIARASEGSVRD
                     ALSRLDQALSVAPEGVTADAVAQALGLGGLDPFFELGDAIAARSPKQALETMDRLYDG
                     GMDVEEIADGLAHHLRQLMLIAVDRSLEKLVDAAPSDRERYAAQAARFHANDLSAMLA
                     LLLESRGDLRRAETPRALLEVALLELCTLPSASSIDELLRRLAALESRLGGEGGGSGP
                     GGVASQGAPAAAPRRAAPAPPAAAAKPVPPRPTEAHAAGVPPFAGAMAAPDRPSPAAP
                     ARETRAAPAREISAPATPVAPAADVAPISAGTPAGHDAERWKRIVDGVKERKLLLGTC
                     LEEGTFLGLTGTAVRVALAPEHSFHKAMLEMKENREILNQEFERQFGRGATLACVVGD
                     PFAATAAAERVRGELQADASAETPEGAAAASVPRPPAAAPSIVQRIVEIFDGEILDLS
                     GETGAPGNGVA"
     gene            2500..2811
                     /locus_tag="VFM00_09475"
     CDS             2500..2811
                     /locus_tag="VFM00_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET7498517.1"
                     /translation="MNIQAMLKKAQALQAKMTQVQEELKTREVIGSSGGGKVKVTMNG
                     AQEVTAIAIDPEVVKPDEADLLEDLVLAAFRDARGKVTKMVEEEMGKVTAGAGLPPGL
                     L"
BASE COUNT          450 a         1006 c         1031 g          415 t
ORIGIN      
        1 tgaatcgggc ggtctcgaaa accgctatac gggtaaccgt atcgagggtt cgaatccctc
       61 tctctccgcc attttgacgc ccgtcgcgcg agggagcgcg cgtgcgcctt atagaagctc
      121 cccggagaga tgtccgagtg gctgaaggag cacggttgga aaccgtgtaa gcatgaaagt
      181 gcttcgaggg ttcgaatccc tctctctccg ccatctgtgc cgtttggtgt tcgggaaacc
      241 gtcgtatcgt ggggatgtag ctcagttggg agagcgcctc gttcgcaacg aggaggtcac
      301 cggttcgatc ccggtcatct ccaccatgat tcacgcagga tccatgtggg aagcaccccc
      361 gcaccggcgc gatgcgccgc gcgcgggggc cccgagttcg gaagtggcct cctcgcgact
      421 cggcgttgac gatcagttcc cgcgcaattc agagtcacga accccgccag gaccggaagg
      481 tagcagcggt cagtgatcga ctggatgtgc cgcggatccg actggtccga gccgggaccg
      541 ggggggccgt ttccctatcc cgtccgcgcg gccgcgggcc gctgaggagc gatcggtgac
      601 tcacctggca ctcgcccgca agtatcgccc gcagcgcttc gccgacctcg tcggccagga
      661 ccccgtgcgc accacgctcg agcgcgcggt cgccaccaac cgcgtggccc acgcctatct
      721 cttcgccggc ccgcgcggca gcggcaagac caccaccgcg cgcctggtcg ccaaggcgct
      781 caactgcgag caccggaagg acggcgaatc ggagccgtgc aaccagtgcc gctcctgcgt
      841 cgagatcacg tcggggacgt cgctcgacgt gctcgagatc gacggcgcct ccaaccgggg
      901 gatcgaggag atccggaatc tccgcgagaa cgtgaagtac gccccctcgg gcgggaagtc
      961 caaggtctac atcatcgacg aagcgcacca gctgaccgac ttcgcctgga acgccctctt
     1021 gaagacgctg gaagagccgc ctccgcacgt gcggttcgtc ttcgccacca ccgagccgct
     1081 cgacgtgccc gacaccatcg cctcccgctg ccaggtcttc gagttccgga ggctccggag
     1141 cgaggagctg gtggctcatc tggagacgat cgccgccgcc gagaaggtga agctggatcc
     1201 ggaagccgcc gcgctcatcg cgcgcgcgtc ggagggctcg gtgcgggatg cgctctcgcg
     1261 gctggaccag gcgctctcgg tcgcgcccga gggggtgacc gccgacgccg tggcgcaggc
     1321 gctgggcttg ggcgggctgg atcccttctt cgagctgggt gacgcgatcg ccgcgcgcag
     1381 cccgaagcag gcgctggaga cgatggaccg actgtacgac ggcgggatgg acgtggagga
     1441 gatcgccgac ggtctcgccc atcacctgcg ccagctcatg ctgatcgcgg tggaccgctc
     1501 gctggagaag ctggtggacg cggccccgtc cgatcgcgag cgctacgccg cgcaggccgc
     1561 ccgcttccac gccaacgacc tgagcgccat gctcgcgctc ctgctcgaga gccggggtga
     1621 ccttcgccgc gccgaaacgc cgcgcgcgct gctcgaggtc gcgctcctcg agctgtgcac
     1681 gctcccgtcg gcctcctcga tcgacgagct gctccggcgc ctcgccgcgc tggagagccg
     1741 tctgggcggg gaaggcggcg gaagcggtcc cggcggcgtc gcgtcgcagg gtgcgccggc
     1801 cgcggccccg cgccgcgcgg caccggcccc gccggcagcg gccgccaagc ccgtcccgcc
     1861 gcggcccacc gaggcgcatg cggccggcgt gcctccgttc gccggtgcga tggcggctcc
     1921 ggaccgcccc tccccggcag ctccggcgcg cgagacccgt gcggctccgg cgcgcgagat
     1981 atccgctccg gccacgcctg tggctccggc cgcagacgtg gctccgattt ccgccggcac
     2041 gccggcgggg catgacgccg agcgctggaa gcggatcgtg gacggcgtga aagagcgtaa
     2101 actcctcctc ggcacctgcc tcgaggaggg gacgttcctc ggcctgacgg gcaccgcggt
     2161 ccgggtggcc ctcgctccgg agcactcctt ccacaaggcg atgctcgaga tgaaggagaa
     2221 tcgggagatc ctgaaccagg aattcgagcg gcagttcgga cgaggcgcca cgctcgcctg
     2281 cgtcgtgggc gatccattcg cggcgaccgc agcggccgag cgggttcgcg gcgagctgca
     2341 ggccgacgcg tcggcggaga cgccggaagg agccgcggcg gcttccgtcc cgcgcccgcc
     2401 ggcggcggcg ccgtcgatcg tgcagcggat cgtcgagatc ttcgacgggg agattctcga
     2461 cctgagcggc gagacgggcg cgccggggaa cggagtggca tgaacatcca ggcgatgctg
     2521 aagaaggcgc aggcgctcca ggccaagatg acgcaggtcc aggaagagct gaagacccgc
     2581 gaggtgatcg gctcctcggg cggcggcaag gtcaaggtca ccatgaacgg cgcgcaggag
     2641 gtgaccgcga tcgcgatcga tcccgaggtg gtgaagccgg acgaggcgga cctcctcgag
     2701 gacctggtcc tggccgcttt tcgcgatgcc cgcggcaagg tgaccaagat ggtcgaggag
     2761 gagatgggca aggtcaccgc gggggcgggc ctgccgccgg gactcctgta gcccgggatg
     2821 cagtactctt ccgctctcct cgaagccctc atcgccgagc tcacccgcct ccccgggctc
     2881 ggccggaagt cggcgcagcg ga
//
LOCUS       DASSLZ010000251        12452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_208648, whole genome shotgun sequence.
ACCESSION   DASSLZ010000251 DASSLZ010000000
VERSION     DASSLZ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208648"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VFM00_09480"
     CDS             <1..674
                     /locus_tag="VFM00_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HET7498518.1"
                     /translation="MRGLRFSLRHEEIFRTQVRAILRATACGNVRIMFPMVVGLEDFR
                     RARAIVTRVRRQLAHEGVPMAEQVPLGVMVETPASVFAIDLIAREADFVSIGTNDLIQ
                     YTLAVDRGNESLAEVYEPLHPAVLRAIRTVIEAGQRFGIRVGICGEMAGEPLYAVLLL
                     GLGVADLSVSPYLLPEIKTILRASMYEEAVSLAQRCLSLSTPSEVRTIVTDFMSRRFP
                     QYFSA"
     gene            713..1756
                     /locus_tag="VFM00_09485"
     CDS             713..1756
                     /locus_tag="VFM00_09485"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HET7498519.1"
                     /translation="MKRRARLDPQGMAARIESFPEQIRAGASIAAPALRGITPGVPRR
                     LVVFGMGGSAIAGDLLRAIADREGVAPVQVVRHYLPPAWLSPEDFLVFSSYSGETEET
                     LSAFEATRSIGARGCVLSTGGTLAAQAGAAGIPAAFLPPGHPPRAALGYSFATLAAVA
                     SHVGVVPGLGDRMEGAAQAVERVVSDCGTRVLQSRNLAKKLAIRLAGRAVLVIGAERG
                     LAAVAQRWKGQLNENAKQLAWASVLPEMNHNEVDGFAGPKAAVGRLAVVLLREPDEHP
                     RIASRFAWLTAYLKRRGVVAVPVLVPGEDAAARLLEASALGDFVSYYLALANGVDPSA
                     LPGVESLKEALRR"
     gene            1753..2736
                     /locus_tag="VFM00_09490"
     CDS             1753..2736
                     /locus_tag="VFM00_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET7498520.1"
                     /translation="MRPVAVIPVAGVGTRLKPHTITTPKALLTVAGKPILGHILDQIA
                     LVSPERVVLVIGPGPHGERIRAYGASRTDLAVSCVMQEEPLGLGHAVSRAAEAVGKAP
                     SLILLGDTIIRADLRGLVTGGSKVGVREVEDPRRFGVAEIENGRIVSLVEKPEHPKSN
                     LALVGLYYLEDSPLLFSCLEQVERSGKRTKGEIQLTDGLAEMLARGADLRPFPVEGWY
                     DCGKTDALLETNRALLDVAGNHVERRGVIILPPVALDPTAEVTHSVIGPHASIGARAK
                     VSRSVIKNSIVNDGAVVEDVLLDLSVIGENALVRGGYHRLNVGDSSEVEVS"
     gene            2796..3929
                     /gene="metK"
                     /locus_tag="VFM00_09495"
     CDS             2796..3929
                     /gene="metK"
                     /locus_tag="VFM00_09495"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET7498521.1"
                     /translation="MLFTSESVTEGHPDKVADQISDAVLDGILGQDPFGRVACETLVT
                     TGLALVAGEITTKCYVDIPRIVRETIRSIGYDDPQYGFDWETCSVLTAIDEQSRDIAQ
                     GVDRRGAGDQGLMFGYAVRETPELMPLPIMLAHRLTKALADARRSGKFPEIRPDGKSQ
                     VTVEYEDDVPKRIDTVVLSTQHAEMGDAEIARLRDRIIDEIIRPALPKGLVDKDPVYH
                     INPTGRFVLGGPRADTGLTGRKIIVDSYGGFGGHGGGAFSGKDPTKVDRSGSYAARHV
                     AKNLVGSGLADRVTVQVAYAIGVEEPVSVMVDSHGTGKVPDARLGELVRKHFDLRPQA
                     IIHALDLRKPIYRRTAAYGHFGRDNEGFKWEILDRVEELKRDA"
     gene            3943..5202
                     /gene="ahcY"
                     /locus_tag="VFM00_09500"
     CDS             3943..5202
                     /gene="ahcY"
                     /locus_tag="VFM00_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7498522.1"
                     /translation="MTPQGHVANPKLAEEGLRRIEWSEREMPVLRQVRERFLKEKPFR
                     GRRVAACLHVTTETANLMTTLKAGGAEIALCASNPLSTQDDVAAALAHRLEIPTFAIK
                     GEDAETYYKHIAACLDVRPEFTMDDGADLVTVLHTQRAADAKGVIAGTEETTTGVIRL
                     RAMAKDGALRYPILAVNDAKTKHLFDNRYGTGQSTIDGVLRATNRLLAGSKFVVLGYG
                     WCGRGLASRARGMGAHVIVTEIDPLPALEAVMDGFPVMPASEAAKVGDVFVTVTGNLN
                     VIRMEHFRLMKDGAIVANSGHFNAEIDLEALAKEASATREVRPFVQEFTLGAKRVFVL
                     GEGRLINLAAAEGHPASVMDMSFANQALGLEYLQTHAKELKPNVYAIPAPVDAEIARL
                     KLEALGVRIDTLTPEQVAYLASWQQGT"
     gene            5219..7573
                     /locus_tag="VFM00_09505"
     CDS             5219..7573
                     /locus_tag="VFM00_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET7498523.1"
                     /translation="MWLLGRVPVRRKYQILGLCLLSVALFVALALFTRDARDTTADLL
                     STGAVRNQGGVVGAFAASGLVAFLGLAGAWLAPMVLLAWGWNRIRLRPAGDLALRTGI
                     GLCGAVVALGLLDLLSGGNRGLAGRLGEWIGLVATRLLGRVGSELALGTALVVIGVVA
                     FELGASSPLRRAAAALLARILPAPAPKKRAAAAPAEETPAPELPRRGRKAPIVEEEEA
                     LASAFAPPAGEAAARRESKPRIVSRNEARAESMSIPFPPPSPAAPEKRRTAPATVGGL
                     GSGPGVPGSAGAPEAGLTLAPAPAMDAALPPVDLLDRHESKTHVVDEAELLELSRVLE
                     RTLADFDVAGKVSEVHPGPVITLFEYEPAPGVKVNQILNRQDDLALSLKAQRIRIVAP
                     IPGKAAVGIEIPNRKKSLVSFREIVTSKTFQESGDALPFALGKDVAGEPFTTSLEKMP
                     HLLIAGATGSGKSVCINTLIMSLLMTRTPAELRFIMIDPKMLELTPYNKIPHLAMPVV
                     TEPKKAAQALRYCVKQMETRYQLLAKHGARNIESYNRLGLDPAVAGADAKLPYLVVVV
                     DELADLMALLPAEIEEPIGRLAQMARAVGIHLILATQRPSVDVITGMIKANFPSRIAF
                     QVASRTDSRVILDMNGAESLLGHGDSLFLPAGKPEPYRIHGSYVSGEEIERVVGFLKA
                     QGGQVVSDDSALEQVRALEDSESDDELFEEAMRLVVLHQQASTSMLQRRLKVGYSRAA
                     RLLDLLEERGVVGPSEGAKGREVLVTEHELAERRAAHASLDEDA"
     gene            7570..8994
                     /gene="rimO"
                     /locus_tag="VFM00_09510"
     CDS             7570..8994
                     /gene="rimO"
                     /locus_tag="VFM00_09510"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET7498524.1"
                     /translation="MSVPIELTAAGDSVRAHAAPRVDDGAATSRVALVTLGCAKNLVD
                     SEIMAGLLASGGFTTTASPEEADVAVVNTCAFIGPAQKESIDRILELAALKREGRLRG
                     LIVAGCLAQRFQGEILREIPEVDAVVGTGQVEAIVRVAHRVRRGGATPLLEVGPAGTA
                     VDLAAHRAVSTPRHLAYLRIADGCDFRCTFCIIPTLRGDLRSRPLESVVEEARRLAEA
                     GTKELLLIAQDSTSYGADRYGTSRLPDLLRALAGVPGIEWIRVHYTHPKTWNEELIRA
                     FEEEPKICRYVDIPLQHVTDAMLRRMRREVRRADTERLMETLRSRLPGVAIRTHFIVG
                     FPGEADEDFEELLSTVERAAYDHVGCFAYSREEGTPAGRMKEQVPERVKLERRRRLLT
                     AQRGVAARVWGAWVGREVTARVDGPGAKDGVWEGRVEQQGYEVDGKTLIRTRSGAAPL
                     LPGDRVTVRIRAVRGYDLDGEVLT"
     gene            8991..9710
                     /locus_tag="VFM00_09515"
     CDS             8991..9710
                     /locus_tag="VFM00_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498525.1"
                     /translation="MKGRVLGWKPRAALVTLGAFALLGLGWGPAPARAYVPTHGMMLD
                     SLAATVAADLLRGQPTDRAVVVLIPLEGDTSGLLGQRLLEKLRSVTSDVRVRERPLAQ
                     PGAQSPTMPPGMPPPSVPVSDSGSAPLELHARLDGAGVSYVRRIRSFPFGVKGYERLV
                     TMRASATMQDPATGRVVWAQSASGSLQDVVRKRDLAYVTGGSTGWNPPLPQGTGPRIL
                     EPLIVVGVVAGLVVLFYSNRN"
     gene            9725..10468
                     /locus_tag="VFM00_09520"
     CDS             9725..10468
                     /locus_tag="VFM00_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7498526.1"
                     /translation="MNAGRVGMSLAARQHQVLGRDYFDRGQYADAIREIEEALRLVPD
                     FPDLHNQLGLALGMSGERERSVAAFRRALELNPQYLEARLNLAIVYNELGRYEEALEE
                     FAVERPRDPDHENLSPDVRTFLADSHVLLGDTYRDLGMPVDATQEYRKALKLAPQFLD
                     VKNKLGTVYCGMGLYADAEAELRSALAQNPRYVDARTTLGLVYYRSGKRHRAREEWER
                     CLMERDGDVRARAYLEMLEREEGADPLPS"
     gene            10465..11238
                     /gene="bamD"
                     /locus_tag="VFM00_09525"
     CDS             10465..11238
                     /gene="bamD"
                     /locus_tag="VFM00_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET7498527.1"
                     /translation="MIRRARFQLRRAAPFLALLLSVFGCGAAALQERSGGTASFESGK
                     SAYDRGDWIQAQLDLKAYVEQYPGTDRTDDALYYLGLAYFKSKDYALASTQFDRLSRD
                     FPQSPFQPDALFYLARCDDLDSRPAPLDQTETQRAIDRYKQFLDAYPDHAHAAEAKQR
                     TQALRDRLAEKRFRNGRLYAKLKQPQAAAIYLRELIDDYPESRWSGDAAVLLADVLVK
                     LGRKQEAIEALRRVPPSATDEVKSRAAGELRELGSSATP"
     gene            11235..11849
                     /gene="nadD"
                     /locus_tag="VFM00_09530"
     CDS             11235..11849
                     /gene="nadD"
                     /locus_tag="VFM00_09530"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET7498528.1"
                     /translation="MRRRGRRRVGILGGSFDPVHVGHLILAEAALEHLRLDRLLLLPA
                     GRPAHKRRRALAPAEDRVAMLRLAARANPRLEVSTVEAARAGVTFTVRTLEALAAAEP
                     ADYFFLMGEDSLREFGTWREPGRILELAKLAVVRREGTPHADVRGALRRRVVFVPMPG
                     VGISSSAIRRRVARGASVRYWVPDAVLTYMDRHGLYRSRRTRRA"
     gene            11812..>12452
                     /locus_tag="VFM00_09535"
     CDS             11812..>12452
                     /locus_tag="VFM00_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET7498529.1"
                     /translation="MDSTEAGERAGRSLLLVDGSALLYRSHFAFLRNPLKNSRGEVTT
                     VTFGMLQALLPLLDKQKPDRMAVVFDTVAPTFRHRVYPEYKAHRPKMPDDLACQIPRV
                     RDAIRFLGVPIVEQEGVEADDLIGSLACEAAREGAMTLILTSDKDFFQLVNDRIVLLA
                     PRGRGGGEAITVDRAGVRERYGVDPGQIVDLLALMGDASDNVPGVPGVGEKTAA"
BASE COUNT         1822 a         4346 c         4452 g         1832 t
ORIGIN      
        1 gcatgcgcgg gctccgcttc tcgctccggc acgaggagat cttccgcacc caggtgcggg
       61 cgatcctgcg cgccaccgcc tgcgggaacg tgcggatcat gttcccgatg gtcgtgggtc
      121 tcgaggattt ccgccgcgcg cgcgccatcg tcacgcgcgt gcggcggcag ctggcgcacg
      181 agggcgtgcc gatggccgag caggtgccgc tgggcgtgat ggtggagacg cccgcctcgg
      241 tcttcgcgat cgacctgatc gcgcgcgagg cggacttcgt cagcatcggc accaacgacc
      301 tgatccagta caccctggcc gtcgaccggg ggaacgaatc gctggccgaa gtctacgagc
      361 cgctccatcc cgcggtgctc cgcgccatcc gcaccgtgat cgaggcgggg cagcggttcg
      421 gcatccgcgt ggggatctgc ggcgagatgg cgggcgagcc gctctacgcg gtgctccttc
      481 tgggcctggg cgtcgccgac ctgagcgtga gcccctacct cctgccggag atcaagacga
      541 tcctccgcgc ctcgatgtac gaagaggcgg tgagcctggc gcagcgctgc ctcagccttt
      601 ccaccccctc ggaggtgcgc acgatcgtga ccgacttcat gagccgccgc ttcccgcagt
      661 acttctcggc atgacgcgcg atgcgcgaaa gcgcgtcgcg ccactcgatg acgtgaagcg
      721 acgcgcccgc ctcgacccgc aaggaatggc ggcgcgcatc gagtccttcc cggaacagat
      781 tcgcgccggc gcatcgatcg cggccccggc cttgcgcggc atcacgcccg gcgtgccgcg
      841 gcggctggtc gttttcggca tgggcggctc cgcgatcgcc ggggacctgc tgcgcgccat
      901 tgccgaccgc gagggggtgg caccggtgca ggtggtccgc cactatctgc cgccggcgtg
      961 gctttcgccg gaggatttcc tcgtcttctc ctcctactcc ggagagaccg aagagaccct
     1021 ctccgccttc gaggcgacgc gctcgatcgg agcgcgggga tgcgtccttt cgaccggcgg
     1081 caccctggcc gcgcaggccg gcgcggccgg aattccggcg gcgttcctgc cgccggggca
     1141 cccgccccgc gcggcgctcg ggtattcctt cgccaccctg gccgcggtgg cttcccacgt
     1201 gggcgtggtc cccggcctcg gcgaccggat ggaaggcgcg gcccaggccg tggagcgggt
     1261 ggtttccgac tgcgggacgc gtgtgctaca atcgcgcaac ttagccaaaa aactggcgat
     1321 acgcttggcg ggacgcgccg tcctggtgat cggggcagag cgcgggctgg ccgcggtcgc
     1381 acagcgatgg aagggccagc tcaacgagaa cgccaagcag ctcgcgtggg cttcggtcct
     1441 tcccgagatg aatcacaacg aggtggacgg cttcgccggg ccgaaggccg cggtggggcg
     1501 cctcgcggtc gtgctgctcc gggagccgga cgagcacccg cggatcgcct cgcgtttcgc
     1561 ctggctcacc gcgtacctga agcggagggg tgtcgtggcg gtccccgtcc tcgtcccggg
     1621 cgaggacgcg gcggcgcggc tcctcgaagc gtccgccctg ggagatttcg tgagctatta
     1681 cctcgcgctc gccaatggag tggatccgag cgcccttccg ggcgtggagt cgctgaagga
     1741 agcgctccgc cggtgaggcc ggtcgccgtc attccggtcg cgggcgtggg aacgcgcctc
     1801 aagccgcata cgatcaccac ccccaaggcg ctgctcacgg tcgcggggaa gccgatcctg
     1861 gggcacatcc tggatcagat cgcgctcgtg agtccggagc gcgtcgtgct cgtcatcggg
     1921 ccgggcccgc acggcgagcg catccgggcc tacggcgcgt cgcgcacgga cctcgcggtc
     1981 tcctgcgtca tgcaggagga gccgctcggc ctggggcacg ccgtgtcccg cgcggccgaa
     2041 gcggtgggga aggcgccgag cctgatcctg ctcggcgaca cgatcatccg cgccgatctg
     2101 cgggggctcg tgaccggggg atcgaaagtg ggggtgcgcg aggtggaaga cccgcggcgc
     2161 tttggcgtgg ccgagatcga gaacgggagg atcgtgtcgc tggtggagaa gccagagcac
     2221 ccgaaatcga acctggccct ggtcggcctc tactacctcg aggactcgcc cctcctcttc
     2281 tcgtgcctgg aacaggtgga gcgctcgggc aagcgcacca agggggagat tcagctcacc
     2341 gacggcctcg cggagatgct ggcgcgcggc gcggaccttc gccccttccc ggtggagggc
     2401 tggtatgatt gcggcaagac cgacgccctg ctcgagacca atcgcgcgct tctcgacgtg
     2461 gccgggaacc atgtcgagcg gcggggcgtg atcatcctgc ccccggtcgc gctggacccg
     2521 accgcggagg tgacgcactc ggtcatcggc ccccacgcat ccatcggcgc ccgggcgaag
     2581 gtgtcgcgct cggtgatcaa gaactcgatc gtcaatgatg gcgccgtcgt cgaggacgtg
     2641 ctgctggatc tctcggtgat cggagagaac gcgctcgtcc gcggcggcta ccatcgcctc
     2701 aacgttggag actcttcgga ggtcgaagtc tcgtgagcgc tcccgctacg cagaccgcac
     2761 cggtggcgcg gcagaacccg cgcctgcgcc attccatgct gttcacgtcc gaatccgtta
     2821 ccgagggcca tcccgacaag gtggccgatc agatctccga cgcggtcctc gacggaatcc
     2881 tgggtcagga tcccttcgga cgggtcgcct gcgagaccct cgtgacgacc ggcctggcgc
     2941 tggtcgccgg ggagatcacc acgaagtgct acgtcgacat cccgcgcatc gtgcgggaga
     3001 cgatccgctc gatcggctac gacgacccgc agtacggatt cgactgggag acctgctcgg
     3061 tcctgacggc gatcgacgag cagtcgcgcg acatcgcgca gggcgtggac cgccgcggcg
     3121 cgggggacca gggattgatg ttcggctacg ccgtgcggga gacgccggag ctgatgccgc
     3181 ttccgatcat gctggcgcac cggctgacga aggcgctcgc cgacgcgcgc cggtcgggga
     3241 agttcccgga gatccggccc gacggcaagt cgcaggtcac ggtcgagtac gaagacgacg
     3301 tgccgaagcg gatcgacacg gtcgtgctct cgacccagca cgcggagatg ggggacgccg
     3361 agatcgcccg gctccgcgat cgcatcatcg acgagatcat ccggccggcc ctccccaagg
     3421 gactggtcga caaggatccg gtctatcaca tcaaccccac gggccggttc gtcttgggcg
     3481 gcccgcgcgc ggacacggga ctgaccggcc ggaagatcat cgtcgacagc tacggcggct
     3541 tcggcgggca cggcgggggc gccttcagcg ggaaggatcc gacgaaggtg gaccgttcgg
     3601 gctcgtacgc ggcgcggcac gtggcgaaga acctggtggg ctccgggctg gccgaccgcg
     3661 tgacggtgca ggtggcctac gccatcggcg tcgaggagcc ggtctcggtg atggtggaca
     3721 gccacggcac cggcaaggtg ccggacgccc ggctcggcga gctggtgcgg aaacatttcg
     3781 acttgaggcc gcaggcgatc atccatgcgc tcgatttgcg gaagccgatc taccggcgca
     3841 ccgcggccta cggacatttc ggacgcgaca acgaggggtt caagtgggag atcctcgacc
     3901 gcgtggagga attgaagcgt gacgcctgag acgatggtcg ccatgacgcc gcagggacat
     3961 gtggcgaacc ccaagctggc cgaagaagga ctccgtcgca tcgagtggtc ggagcgggag
     4021 atgccggtcc tgcgccaggt gcgcgagcgc ttcctgaagg aaaagccgtt ccgcggacgg
     4081 cgcgtcgccg cctgtctgca cgtcaccacc gagaccgcaa atctcatgac gacgctcaag
     4141 gccggcggcg cggagatcgc gctctgcgcc tcgaatccgc tctcgacgca ggacgacgtg
     4201 gccgccgcgc tcgcgcaccg gctggagatc ccgacgttcg cgatcaaggg cgaggacgcc
     4261 gagacctact acaagcacat cgcggcctgc ctcgacgtgc gtccggaatt caccatggat
     4321 gacggcgccg acctggtcac cgtgctccac acccagcgcg cggccgacgc caagggcgtc
     4381 atcgccggga ccgaggagac caccaccggc gtcatccgcc tccgcgccat ggccaaggac
     4441 ggggcgctcc gctacccgat cctcgccgtg aacgatgcca agacgaagca cctcttcgac
     4501 aaccgctacg ggaccgggca gagcaccatc gacggcgtgc tccgcgccac gaaccggctc
     4561 ctggccggct ccaagttcgt ggtgctgggc tacggatggt gcggccgggg cctcgcgagc
     4621 cgcgcccggg gcatgggcgc gcacgtgatc gtgaccgaga tcgatccgct ccccgcgctc
     4681 gaagcggtga tggacggctt tccggtcatg cccgcctccg aggccgcgaa ggtcggcgac
     4741 gtgttcgtca ccgtcaccgg gaacctgaac gtgatccgca tggagcactt ccgcctgatg
     4801 aaggacggcg ccatcgtcgc caactccgga cacttcaacg cggagatcga cctcgaggcg
     4861 ctggcgaagg aagcctccgc cacgcgggag gtgcggccct tcgtgcagga gttcacgctc
     4921 ggcgccaagc gggtgttcgt cctgggcgaa ggaaggctca tcaacctcgc cgccgccgag
     4981 gggcatcccg cctccgtcat ggacatgagc ttcgccaacc aggcgctcgg gctggaatat
     5041 ctgcagacgc acgcgaagga gctgaagccg aacgtctacg ccattcccgc tccggtcgac
     5101 gccgagatcg cgcgcctgaa gctggaagcg ctcggagtcc gcatcgacac gctgacgccc
     5161 gagcaggtcg cgtacctcgc gtcctggcag cagggaacgt aggagcgacc acaccggcat
     5221 gtggctcttg ggacgggtgc ccgtccgccg caagtaccag atcctggggc tctgccttct
     5281 ttcggtcgcc ctcttcgtgg cgctcgccct cttcacgcgc gacgcccgcg acaccacggc
     5341 cgatctcctc tccacgggcg ccgtccggaa ccagggcggg gtcgtcggcg cgttcgccgc
     5401 ctcgggcctg gtcgccttcc tcggtctcgc gggagcctgg ctcgcgccga tggtcctcct
     5461 cgcctggggg tggaaccgga tccggctgcg ccccgcgggc gatctcgcgc tgcgcaccgg
     5521 gatcgggctc tgcggcgccg tggtcgcgct gggactcctc gacctcctct cgggcggaaa
     5581 ccgcggtctg gccggacggc tcggcgaatg gatcgggctc gtcgccacgc ggcttctcgg
     5641 gcgcgtgggg agcgagctcg cgctcgggac ggcgctggtg gtgatcggcg tggtcgcctt
     5701 cgagctgggg gcttcctcgc ccctccgccg cgcggccgcg gcactgctcg cccgcatcct
     5761 gcccgcgccg gctcccaaga agcgcgccgc ggcagcgccg gccgaggaga cgcccgcgcc
     5821 cgagctgccg cgccgcggcc ggaaggcccc gatcgtcgaa gaggaagagg cgctcgcttc
     5881 ggcgttcgcg cctcccgccg gcgaggccgc cgcgcggcgc gagtccaagc cgcgcatcgt
     5941 gtcgcggaac gaggcgcgcg cggagtcgat gtcgatcccg ttcccgcccc cctcgcccgc
     6001 ggcgccggag aagcggcgaa ccgcgcccgc cacggtgggc ggactgggat ccggccccgg
     6061 cgttcccggc tccgccggtg ctcccgaggc gggcctcacg ctggcgcccg cgcccgcgat
     6121 ggacgcggcg ctccctccgg tggatctcct cgaccgccac gagtcgaaga cgcacgtggt
     6181 ggacgaggcc gagctcctcg agctctcgcg ggtgctcgag cgcacgctgg ccgacttcga
     6241 cgtggcgggg aaggtttccg aggtgcaccc cggccccgtg atcacgctgt tcgagtacga
     6301 gcccgcgccc ggcgtgaagg tgaaccagat cctcaaccgt caggacgatc tcgcgctctc
     6361 gctcaaggcg cagcggatcc gcatcgtggc gccgatcccg gggaaggcgg cggtggggat
     6421 cgagatcccc aatcgcaaga agtcgctcgt ctccttccgc gagatcgtga cgtcgaagac
     6481 gttccaggag agcggggacg cgctgccgtt cgccctcggc aaggacgtgg cgggagagcc
     6541 cttcacgacc tcgctggaga agatgccgca cctgttgatc gcgggcgcga ccggctcggg
     6601 gaagagcgtc tgcatcaaca cgctgatcat gagcctgctc atgacgcgca cgccggccga
     6661 gctccgcttc atcatgatcg atcccaagat gctcgagctc acgccctaca acaagatccc
     6721 gcacctcgcg atgccggtcg tgaccgagcc gaagaaggcg gcgcaggcgc tccgctactg
     6781 cgtgaagcag atggagaccc gctaccagct cctcgccaag cacggggcgc ggaacatcga
     6841 gtcgtacaac cggctcggtc tcgacccggc ggtggccggc gccgacgcga agctcccgta
     6901 cctcgtcgtg gtggtggacg agctggccga tctcatggcg ctcctccccg cggagatcga
     6961 ggagccgatc ggccggctgg cgcagatggc gcgcgcggtg ggcatccacc tgatcctcgc
     7021 gacgcagcgc ccctcggtgg acgtgatcac cgggatgatc aaagcgaact tcccctcgcg
     7081 gatcgccttc caggtcgcga gccgcaccga ctcgcgcgtg atcctggaca tgaacggcgc
     7141 ggagagcctc ctgggccacg gcgactcgct cttcctcccg gccggcaagc ccgagcccta
     7201 ccggatccac ggctcctacg tgtcgggcga ggagatcgag cgcgtcgtcg gcttcctgaa
     7261 ggcgcagggg gggcaggtcg tgtccgacga ctccgcgctg gaacaggtgc gcgccctgga
     7321 ggattcggag agcgacgacg agctgttcga agaagcgatg cgcctggtcg tgctgcatca
     7381 gcaggcctcc acctcgatgc tccagcgccg tctcaaggtc gggtactcgc gcgcggcgcg
     7441 gctgctcgat ctcctcgagg agcgcggggt ggtcgggccg tccgaggggg cgaaggggcg
     7501 cgaagtgctc gtcaccgagc atgagctggc cgagcggcgc gcggcgcacg cgtcgctgga
     7561 cgaggacgcg tgagcgttcc gatcgagctg accgcggccg gcgactccgt gcgcgcccat
     7621 gccgcgccgc gcgtggatga cggcgccgcc acgtcccggg tggccctggt cacgctgggc
     7681 tgcgccaaga acctcgtgga ctcggagatc atggcgggcc tgctcgcctc cggcggattc
     7741 acgacgacgg cgtcgcccga agaggccgac gtcgcggtcg tgaacacctg cgccttcatc
     7801 ggccccgccc agaaggagtc gatcgaccgg atcctcgagc tggccgcgct gaagcgcgaa
     7861 gggcgcctgc gcggcctgat cgtggcggga tgcctggccc agcgcttcca gggggagatc
     7921 ctccgcgaga tccccgaggt ggacgccgtc gtcgggaccg ggcaggtgga ggcgatcgtg
     7981 cgcgtggcgc accgggtgcg ccgcggcggc gcgacgccgc tcctcgaagt ggggcccgcc
     8041 ggcaccgcgg tcgatctcgc cgcccatcgc gccgtctcga cgccgcggca tctggcctat
     8101 ctccgcatcg ccgacggctg cgacttccgc tgcaccttct gcatcatccc gacgctccgc
     8161 ggggatctcc ggagccggcc gctggagtcg gtggtggagg aggcgcggcg gctggccgag
     8221 gcgggaacga aggagctgct tctgatcgcg caggattcca catcctacgg cgccgaccgc
     8281 tacgggacct cgcgccttcc cgatctcctc cgcgctctcg cgggcgttcc ggggatcgag
     8341 tggatccggg tgcactacac ccatccgaag acctggaacg aggagctgat ccgcgcgttc
     8401 gaagaggagc cgaagatctg ccgctatgtc gacattccgc tgcagcacgt caccgacgcg
     8461 atgctccggc gcatgcgccg cgaggtgcgg cgcgccgaca ccgagcgcct gatggagacg
     8521 ctccggagcc ggctgcccgg cgtggcgatc cgcacgcact tcatcgtggg ctttcccggc
     8581 gaggccgacg aggacttcga ggaactcttg agcaccgtgg agcgcgccgc ctacgaccac
     8641 gtcggctgct tcgcctattc gcgcgaagag gggacgccgg cagggcggat gaaggagcag
     8701 gttcccgagc gggtgaagct cgagcggcgg cggcggctcc tcaccgcgca gcgcggcgtg
     8761 gccgcgcgcg tgtggggcgc ctgggtgggg cgcgaggtga cggctcgggt ggacgggccc
     8821 ggcgcgaagg acggcgtctg ggagggccgt gtggaacagc agggttacga ggtggacggg
     8881 aagacgctga tccggacgcg cagtggggcc gccccgctcc tgccgggcga ccgggtgacg
     8941 gtccggatcc gcgccgtgcg ggggtacgac ctggatgggg aggttctcac gtgaagggac
     9001 gcgtccttgg atggaagcct cgggcggcac tcgtgacgct cggggcgttc gcgctgcttg
     9061 ggctgggatg gggaccggcg cccgcgcgcg cgtatgtccc gacccatggg atgatgctgg
     9121 actccctggc cgccacggtg gccgcggacc tgctgcgcgg ccagccgacc gaccgcgcgg
     9181 tggtggtcct gatcccgctg gagggggaca cctcgggtct cctggggcag cgacttctgg
     9241 agaagctccg ctccgtgacc agcgacgtcc gcgtgcggga gcggccgctg gcgcagcccg
     9301 gcgcccagtc cccgacgatg ccgcccggca tgccgccgcc cagcgtcccg gtctccgact
     9361 cgggatcggc gccgctcgag ctgcatgcgc gcctggatgg cgccggggtc agctacgtgc
     9421 ggcggatccg ttccttcccc ttcggggtga aaggatacga gcggctcgtg accatgcgcg
     9481 cgagcgccac gatgcaggat ccggcgacgg ggcgggtggt ctgggcccag agcgcctcgg
     9541 gctcgctcca ggacgtcgtt cgaaagcgcg atctcgcgta cgtcaccggc ggctcgaccg
     9601 gatggaaccc gccgctgccg cagggcacgg ggccccgcat cttggagccc ctgatcgtgg
     9661 tcggggtggt ggccggtctg gtcgttctct tctactccaa ccggaactga ggagcgggtc
     9721 caccatgaac gcagggaggg tcggcatgag tctggcggcc aggcagcacc aggtgctcgg
     9781 gcgggactac ttcgaccgcg gccagtacgc cgatgcgatc cgcgagatcg aggaggcgct
     9841 ccggctggtg cccgattttc cggatctgca caatcagctc gggctggcgc tcggcatgag
     9901 cggcgagcgg gagcgatcgg tggcggcgtt ccgccgcgcg ctcgagctca atccccaata
     9961 cctggaggcg cggctgaatc tggcgatcgt ctataacgag ctggggcgct acgaagaggc
    10021 gctcgaggag ttcgcggtgg agcggccgcg cgatcccgac cacgagaacc tctcgcccga
    10081 cgtgcgcacc ttcctggccg attcgcacgt gctgctgggg gacacctacc gcgatctcgg
    10141 gatgcccgtc gacgcgacgc aggagtaccg gaaggcgctc aagctggcgc cgcagttcct
    10201 ggacgtgaag aacaagctgg gcacggtcta ctgcggcatg gggctctacg ccgacgccga
    10261 ggccgagctc cgctccgcgc tcgcccagaa cccgcgctac gtcgacgcgc gcaccacgct
    10321 cggcctggtc tactaccgct cgggcaagcg ccaccgcgcc cgcgaggagt gggagcgctg
    10381 tctcatggag cgcgacgggg acgtccgcgc gcgcgcctat ctcgagatgc tggagcgcga
    10441 agagggcgcg gacccgctcc cttcatgatc cggcgcgcac gcttccaact ccgccgcgcg
    10501 gcgccgttcc tggcactcct cttgtccgtg ttcggctgcg gtgccgccgc gcttcaggag
    10561 cgaagcgggg gcaccgccag cttcgaatcg gggaagtccg cgtacgaccg gggggactgg
    10621 atccaggcgc agctcgacct caaggcctat gtcgagcagt accccggaac cgaccgcacc
    10681 gacgacgcgc tctactacct gggcctggcg tacttcaagt ccaaggacta cgcgctggct
    10741 tcgacgcagt tcgaccggct gtcgcgcgac ttcccgcagt caccgttcca gcccgacgcg
    10801 ctgttctacc tggcccggtg cgacgacctg gattcccggc ccgcgccgct cgaccagacc
    10861 gagacgcagc gcgcgatcga ccgctacaag cagttcctgg acgcctatcc cgaccacgcg
    10921 cacgccgccg aggcgaagca gcgcacgcag gcgctgcgcg accgcctggc cgagaagcgg
    10981 ttccggaacg gccgcctcta cgccaagctc aagcagcctc aggccgccgc catctatctc
    11041 cgcgagctga tcgacgacta tcccgagtcg cgctggtcgg gcgacgcggc cgtgctcctg
    11101 gcggacgtcc tcgtgaagct gggccggaaa caggaggcga tcgaggcgct gcggagggtc
    11161 ccgccgtccg cgaccgacga ggtcaagtcg cgcgcggccg gcgagctccg ggagctgggc
    11221 tccagcgcca ccccgtgagg cgccgcggac ggcgtcgcgt cggcatcctc ggcggctcct
    11281 tcgatcccgt ccacgtcgga cacctgatcc tggccgaggc cgcgttggag caccttcggc
    11341 tcgaccgcct cctgctcctg cccgccggcc gccccgcgca caagcgccgc cgcgcgctcg
    11401 ctccggccga agaccgggtc gcgatgctcc gtctggccgc gcgcgccaat ccgcgcctcg
    11461 aagtctcgac cgtggaagcg gcccgcgccg gcgtgacctt caccgtgcgc acgctggagg
    11521 cgctggcggc cgccgagccc gccgactact tcttcctgat gggggaggac agcctgcgcg
    11581 agttcggcac ctggcgcgag cccggccgca ttctcgagct cgccaagctg gcggtggtgc
    11641 gccgggaggg aacgccgcac gccgacgtcc gcggggcgct ccggcggcgc gtggtcttcg
    11701 tgccgatgcc cggcgtcggg atctcctcgt ccgcgatccg gcggcgcgtc gcgcggggtg
    11761 cctccgtgcg atactgggtg ccggacgcgg tgctgaccta catggaccga catggactct
    11821 accgaagccg gcgaacgcgc cgggcgtagc cttctcctcg tcgacggctc ggcgctcctc
    11881 taccgcagcc atttcgcgtt cctccggaac ccgctcaaga attcgcgcgg cgaggtgacg
    11941 accgtcacct tcggcatgct ccaggctctg cttccgctgc tggacaagca gaagccggat
    12001 cgcatggccg tggtcttcga cacggtcgcg cccacgttcc gccaccgggt ctaccccgag
    12061 tacaaggcgc accgcccgaa gatgccggac gacctggcct gccagatccc gcgcgttcgg
    12121 gacgcgatcc gcttcctggg ggtgccgatc gtggagcagg agggggtgga ggccgacgat
    12181 ctcatcggaa gcctcgcctg cgaggcggcg cgcgagggcg cgatgacgct catcctgacc
    12241 tcggacaagg acttctttca gctggtgaac gatcgcatcg tgctcttggc gccgcgcggg
    12301 cgcggaggcg gcgaagcgat cacggtggac cgtgccggcg tgcgggagcg ctacggcgtc
    12361 gatccggggc agatcgtgga tctgctcgcg ctcatgggcg acgcgtccga caacgtcccc
    12421 ggcgtgccgg gcgtggggga gaagaccgcg gc
//
LOCUS       DASSLZ010000252         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_600017, whole genome shotgun sequence.
ACCESSION   DASSLZ010000252 DASSLZ010000000
VERSION     DASSLZ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600017"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1409
                     /locus_tag="VFM00_09540"
     CDS             150..1409
                     /locus_tag="VFM00_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498530.1"
                     /translation="MKRLAILFALAVALGAAVMGADPGPADAAARGRARAAAAKGQAH
                     DSATAKGSLSQLLAPAYERTARTWPKVGGGFEGRRIVTYLERRDTRKMDLAAARFVPR
                     ADSLAARHREASFDPRDAIYSTFRVGADTLRFTWGHMAEEFGGVPRVILAGPGAAPRA
                     FLLAPLAHWNVDALWVVDEYIVFGCASESEGGGIAEQIVVWNLATGRWFGTPSESALA
                     HRGFKLRDFFPAWRTAQAAVVQGSVVLQGSDRALALDPKAGSWSLVSIAGLAIGPNVH
                     QIARRLIPVTEAMRSRLLPQLLGAFQERNREVDSVQVLEVMQDPCAAQRGRAAVLAMG
                     LAPSKATAAQSTDIATTLNRQLFGIFLADSTASTVMAKIDMFPTVAYLDYVVYFDLEA
                     ADDAIVIWGQGARYGGDEAQRGYRCGN"
     gene            1397..>2262
                     /locus_tag="VFM00_09545"
     CDS             1397..>2262
                     /locus_tag="VFM00_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498531.1"
                     /translation="MRELSDSIRDHIARADWTPTGDAQARRTEELMIRSFRAQIESIP
                     PYGAFVRHLGVDPARVERWQDIPPVPCSAFKSHELCSATPDETAGVTFETSGTTISQP
                     GRVRLADTTLYETSLLRNFERHLLPDGPRLKAIVFGPSAADAPHSSLWFMVDRVAREY
                     GLEPLYVVQHGDPRWKAADDAIRLAMEKREPLLLLGTTLLFMAYFERLRILNAAFQLP
                     PESRAMDTGGAKGQIAEFTRAEVEEAFERHLALPPSHVVNEYGMAEMGSQFYEDSLLA
                     EHQRREAIPGTHL"
BASE COUNT          343 a          786 c          776 g          357 t
ORIGIN      
        1 tcccgacgcg ctcgtgctcc tgggcgaagg ctgggacgag ctcctcgact tcctcgatcg
       61 ccgcttcgag ttcggccccg acctgcgcgg gctgctccgc gtggcgcacg acgcggaaga
      121 agccgcgcgg atcgcgtggg gagtatgagg tgaaacggct cgcgatcctc ttcgccctcg
      181 cggtcgctct cggagcagcg gtcatgggcg ccgacccggg tcccgcggat gccgccgcta
      241 ggggtcgcgc ccgtgccgcc gccgccaagg gtcaagccca cgactccgcc accgccaagg
      301 gctccctttc ccagttgctg gcccccgcct acgagcgcac ggcacgaacc tggcccaaag
      361 tcgggggagg gttcgaaggc cgccggatcg tgacctatct ggagcgccgc gatacgcgga
      421 agatggatct cgccgcagcg cgcttcgttc cccgcgccga cagcctcgcc gcgcgacatc
      481 gcgaggcatc gttcgatccc cgggacgcga tctacagcac gtttcgcgtg ggggccgaca
      541 cgcttcgctt cacatggggc cacatggcgg aggaattcgg cggcgtgcct cgggtgatcc
      601 tggctggccc aggcgcggcg ccacgcgctt tccttcttgc gcccctagcg cattggaacg
      661 tggacgcact ctgggtcgtg gacgaataca tcgtattcgg atgcgccagc gaatccgaag
      721 gcggggggat cgccgagcag atcgtggttt ggaatctagc caccggccgt tggttcggaa
      781 ctccgagcga atcggccctc gcgcaccgcg ggttcaagct tcgggacttc ttcccggcat
      841 ggcgtacggc ccaggcggcg gtcgtgcagg gctcggtcgt gctccagggg agcgaccgcg
      901 cgttggcgct cgatcccaaa gcgggctcct ggtcgctggt gagcatcgcc ggcctcgcga
      961 tcgggccgaa cgtccaccag atcgcgcgtc gtctgatacc cgtcaccgaa gccatgcgct
     1021 cgcgcctgct cccgcagctg ctcggcgcct tccaggaacg gaatcgcgag gtcgacagcg
     1081 tccaggtcct cgaggtgatg caggacccgt gtgccgcgca gcggggacgg gccgccgtgc
     1141 tcgcgatggg gttggcgccg tcgaaggcga cggcggccca atccaccgat atcgcgacga
     1201 cactgaatcg ccagctcttc gggatcttcc tggccgacag cacggcctcc acggtcatgg
     1261 cgaagatcga catgttcccg accgtcgcct atctcgacta cgtcgtgtac ttcgacctgg
     1321 aggccgcgga cgacgcgatc gtcatctggg gccagggggc gaggtacggc ggggatgagg
     1381 cccagcgagg ataccgatgc gggaactgag cgattccatc cgcgaccaca tcgcgcgcgc
     1441 ggactggacc ccgaccggcg acgcgcaggc ccggcggacc gaggagttga tgatccgctc
     1501 gttccgcgcg cagatcgaat cgatcccgcc gtacggcgcg ttcgtgcgcc acctgggcgt
     1561 ggatcccgcg cgggtcgagc gctggcagga cattcccccc gtgccctgct ccgcgttcaa
     1621 gagccacgag ctttgctccg cgactcccga cgagaccgcc ggcgtcacgt tcgagaccag
     1681 cggcacgacg atctcccagc cggggcgcgt gcgcctcgcg gacacgacgc tctacgagac
     1741 ttcgctgctc cggaatttcg agcggcacct cttgcccgac ggcccgcgcc tgaaagcgat
     1801 cgtcttcggc ccgagcgcgg ccgacgcgcc gcattcctcc ctctggttca tggtggaccg
     1861 cgtggcgcgc gagtacgggt tggagccgct ctacgtggtg cagcacggcg atccgcggtg
     1921 gaaggccgcg gacgacgcga tccggctcgc gatggagaag cgcgagccgc ttctccttct
     1981 ggggacgacg ctcctcttca tggcctactt cgagcgcctc cggatcctga acgccgcgtt
     2041 ccagctcccg cccgaaagcc gcgccatgga tacgggcgga gcgaaggggc agatcgccga
     2101 gttcacgcgc gccgaggtgg aagaggcgtt cgagcggcac ctggcgctcc ccccctcgca
     2161 cgtcgtgaac gagtacggca tggcggagat ggggagtcag ttctacgagg actcgctgct
     2221 tgcggagcac cagcggcggg aagcgattcc cgggacacac ct
//
LOCUS       DASSLZ010000253         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1613218, whole genome shotgun sequence.
ACCESSION   DASSLZ010000253 DASSLZ010000000
VERSION     DASSLZ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613218"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1123..1587
                     /locus_tag="VFM00_09550"
     CDS             1123..1587
                     /locus_tag="VFM00_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498532.1"
                     /translation="MPGDYRYLDESTLVASTYWGTISLVDILETITHRAGDLAEHHPK
                     ASVVDLSAAKWHETPPKFMHHQIERLRPALAPPKVRTILIAPSDFFYGFARMYAIMHN
                     VYGAANVEVARSWADAAHILGMDLSSAENWSRERVAQEEAAQAADTTRMPKP"
     gene            1584..2009
                     /locus_tag="VFM00_09555"
     CDS             1584..2009
                     /locus_tag="VFM00_09555"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET7498533.1"
                     /translation="MSAGQSVRARATRRFDAPAERVYAAWLDPAKIRDWFGPGLGPMV
                     RIDVEPRVGGRFSWVQRRGDKDVEHVGAFLAMDPPRRLAFTFAVPPDPESRVFVDLAQ
                     RGEGCEVTVTHEIPAEWAAFIPKSIEAWSKMLDAMAGTV"
     gene            2025..>4036
                     /locus_tag="VFM00_09560"
     CDS             2025..>4036
                     /locus_tag="VFM00_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498534.1"
                     /translation="MAPNSSKVALGPAVFATAAGMFASQVGGRATRDALFLSHFPVTA
                     LPVMIMATAVLALALAYVSTRAMVRWGPAVVMPAAFALSAALLVVEWGVSYVAPGVAA
                     VLVYLHFGGLGALLISGFWSFLNERLDPRSAKRQLGQVNAAGTLGGLGGGLLAAELAR
                     HIPVAAMLPVLAGIQVVCAAGMVQLRARGEREARAVGDAQRGAGAAPGGSGRAPDEAK
                     PQSPRQAGASTAPATSTSPRQAGASTAPATSTANLRALLSMPYIRNLIALVVLLTVAD
                     VMIDLVLKGRAALVWPHGAGLLRFFAAFYTGVSLLIFLVQAFVSRFVLERVGPARAAA
                     ILPAGTAIGAAGAFVAPGLTAAAIARGTQSVLMASLFNAGYEVLFTPVPARDKRAIKS
                     LVDVGASRAGDLLGAAIAQAVVFMPMALASRGNVLLALAVALSGVAFFLTLRLQRGYV
                     LALARGLVTRAVHLELSEATDSITRSTLLKTLQISRPVERARVEGGVQRAARVEGGAP
                     RAARTEGGVQEAATARGTTSSSHTELRYILTGQNQPLEDESPEAQRARALRSRDLETV
                     RGALRQGPIPESLLPQAVSLLAWDPVAQDAISTLRHTGASAIDALVEPILDPDSDFTI
                     RRRVPLVLGTIADPRSVQALLLSLTDRRFEVRYRSGRALAHLHSLDP"
BASE COUNT          579 a         1391 c         1430 g          636 t
ORIGIN      
        1 cgccggttcg actcgcgggc ggtgcgaccc aacgggcccg cgtccacgat tcgccccatc
       61 ccccaacttg cgccagcgca agttcggacg gctctgcaag agggacaact tgcgctggcg
      121 caagtcgaga tcagctctgg attgaatgga gccacgcccg gactgaatgg agccgtgccc
      181 ggattgaatg gagccacgcc cggactgaat ggagccgtgc ccggattgat cggagccacg
      241 cccgggttga atggagccgt gcccgggttg aatggagccg ggtcagggtc ttcgagcaat
      301 caccctgatc cggctcccgt tgtgcctggc ttggccgttg gaacgccggg cctccagcag
      361 cccagtcccg gcccaagcca gcgcttcctg gtcaagctca cgatcgacaa ggccacccac
      421 gacaagctgc gccgcgcgca agctcttctc agccacgcca ttcccgccgg agacgtggtc
      481 caggtgctcg accgcgccct ggatgctctg atcacgcagc tggagcggcg gaaggccggg
      541 gccgagcgcg ccggccgatc ccgtccgacc atgcggccgc ggtatatccc ggcacggatc
      601 cgtcgcgccg tatgggagcg ggacggcggg cagtgcacgt tcgtcggcgc cgatgggcac
      661 cgatgcaccg cgcgagggtt cctcgagttc gatcacacga aacctgttgc ccggggaggg
      721 gaggcgtccg tggaagggat tcggctccgt tgtcggaccc ataatcaata tgaagccgag
      781 cgcgtgtttg gcgtggcgtt catgaggaga aagcagcggg aggcgcggga ggctgcgccg
      841 gggcaggagg atgagcgggg gctgggggcc gaaccggggc aggcggctgc gccgaggcag
      901 gaggctgcgc cgagacaggc ggatgcgccg agccgaggta ttgcgccgga ccagggcaat
      961 gggccacgcc cattgccgcg agacgctacg gagacgccgc ccgaaccact cccctgatcc
     1021 agccgacatc atctaggcgc gcgcgctggt aagggctact attgggatct cctcggggtt
     1081 tcgagagacc ccgaacccac tctatccagc gggggccgac tcatgcccgg cgactatcgc
     1141 tatctggacg agagcaccct cgtcgcatcc acctactggg gcacgatttc cctggtcgac
     1201 atcctcgaga cgatcacgca tcgggcgggg gaccttgcgg agcaccaccc gaaggcgagc
     1261 gtggtcgatc tttccgcggc caagtggcac gagacgccgc ccaagttcat gcatcatcag
     1321 atcgaaaggc ttcgcccggc gttggcgccg cccaaggtgc gcacgatcct gatcgcgcca
     1381 agcgacttct tctacggctt cgcgcggatg tacgccatca tgcacaacgt ctacggtgcc
     1441 gcgaacgtgg aggtcgcgcg gtcctgggcg gacgcggccc acattctcgg catggatctc
     1501 tcctcggcgg agaattggtc gcgcgagcgg gtcgcgcagg aagaggctgc ccaggcggcg
     1561 gacacgaccc ggatgccgaa gccgtgagcg ccgggcagtc ggttcgcgcg cgcgcgacgc
     1621 ggcggttcga cgccccggcc gaacgcgtct atgcggcgtg gctcgatccc gcgaagatcc
     1681 gcgactggtt cgggccgggc ctgggaccca tggtgcgcat cgacgtggag ccacgcgtgg
     1741 gcggccggtt ctcctgggtg cagcgccgcg gcgacaagga cgtcgagcac gtgggagcgt
     1801 tcctcgcgat ggacccgccc cggcggctgg cgttcacctt cgcggtcccg cccgatccgg
     1861 agagccgcgt tttcgtggac ctggcccagc gcggcgaagg gtgcgaggtg acggtcacgc
     1921 acgagatccc cgccgagtgg gcggccttca ttccgaagag catcgaggcg tggagcaaga
     1981 tgctggacgc catggcgggg acagtctgag gctgcggccc gaccgtggcc cccaactcca
     2041 gtaaagtcgc tctaggcccc gccgtcttcg cgacggccgc gggcatgttc gcctcgcagg
     2101 tcggcgggcg cgcgacgcgc gacgccctct tcctctccca cttcccggtc accgcgctcc
     2161 ccgtgatgat catggccacg gcggtgctcg cgctcgcatt ggcctacgtt tcgacgcggg
     2221 cgatggtccg ctgggggccg gccgtcgtga tgccggcggc ctttgccctg agtgccgcgc
     2281 tcctggtcgt cgagtggggc gtgtcgtacg tggcgccggg cgtcgccgcc gtcctggtct
     2341 atctccattt cggtgggctg ggcgcgctcc tgatctcggg attctggtcg ttcctgaacg
     2401 agcggctcga tccgcggagc gccaagcggc agctgggcca ggtgaacgcc gcggggacgc
     2461 tgggcggcct gggagggggt ctgctcgcgg cagagctggc gcggcacatc ccggtggcgg
     2521 cgatgcttcc ggtgctcgcc gggatccagg tggtgtgtgc ggcggggatg gtgcagttgc
     2581 gggcgcgggg ggagcgagag gccagggcgg ttggtgacgc gcaacggggg gccggcgcgg
     2641 ctccaggcgg ctccggccga gctcccgatg aagcgaagcc ccaatcgccc cgccaggccg
     2701 gcgcctccac agcgcctgcc acctccacat cgccccgcca ggccggcgcc tccacagcgc
     2761 ctgccacctc caccgccaac ctgcgcgcgc tcctctccat gccctacatc cggaatctca
     2821 tcgcactcgt cgtgctgctc accgtcgccg acgtgatgat cgacctggtg ctgaaggggc
     2881 gggctgcact ggtgtggccg cacggggcgg ggctcctgcg cttcttcgcc gcgttctaca
     2941 cgggcgtttc gctcctgatc ttcctggtcc aggccttcgt gagccgcttc gttctggaac
     3001 gggtcggacc cgcgcgggcg gccgcgatcc tcccggcggg gaccgcgatc ggggcggccg
     3061 gggcgttcgt ggcgcccggt ctgacggccg cggccatcgc gcgcgggacg cagtcggtgc
     3121 tcatggcctc gctcttcaac gccggctacg aggtgctctt cacgccggtg ccggcgcgcg
     3181 acaagcgcgc catcaagtcg ctcgtggacg tgggcgcctc gcgcgcgggg gacctcctgg
     3241 gtgcggcgat cgcgcaggcc gtggtcttca tgccgatggc gctggccagc cgggggaacg
     3301 tgctgctcgc cctcgcggtc gcgctctcag gggttgcctt cttcctgacg ctccggttgc
     3361 agcgcggata cgtgctggcg ctggcacggg gcctggtcac gcgcgcggtg catctggagc
     3421 tgtcggaggc caccgattcg atcacgcgat cgaccctgtt gaagacattg caaatctctc
     3481 gccccgttga gcgggcgagg gtcgagggtg gcgtccagag ggcggcgcgt gtcgaggggg
     3541 gcgccccaag ggcagcgagg accgagggtg gcgtccagga ggcggcaaca gcacgaggca
     3601 ccacatccag ctcccacacc gagttaagat atatattaac aggacagaac caacccctcg
     3661 aggacgagtc gcccgaagcc caacgagctc gcgcgctccg gtctcgcgat ctcgagaccg
     3721 tccgcggcgc tctacggcaa ggccccattc cggaatcgct ccttccgcag gcggtctcgc
     3781 tcctcgcctg ggaccccgtg gcgcaggacg ccatctcgac gctgcgccac accggcgcct
     3841 cggccatcga cgctctcgtc gagccgatcc tggatcccga ttccgacttc acgatccgcc
     3901 ggcgcgtgcc gctggtcctg ggcaccatcg ccgacccgcg ctcggtccag gcgctcctgc
     3961 tctccctgac cgaccggcgc ttcgaagtgc gctaccgctc gggccgggcg ctggcgcatc
     4021 tccactcgct cgatcc
//
LOCUS       DASSLZ010000254        24606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_474645, whole genome shotgun sequence.
ACCESSION   DASSLZ010000254 DASSLZ010000000
VERSION     DASSLZ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 24606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24606
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474645"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFM00_09565"
     CDS             complement(<1..304)
                     /locus_tag="VFM00_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498535.1"
                     /translation="MVRAAPERRGASWLAPLSTSRDLLRASLHRPAPEAHVRPLASFR
                     LPASALAWMLLAAGHAAGWAAPVPAALRMHALAACAGLGAVSLLAGAAGSRRAGVAR"
     gene            complement(321..2384)
                     /locus_tag="VFM00_09570"
     CDS             complement(321..2384)
                     /locus_tag="VFM00_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET7498536.1"
                     /translation="MALVAGSDPSETPLGRIITALRSIHAELVRRPARDRPWVSVLAA
                     VFHAGDGFVVSAGDCACFRFRDGLLSRLERPGDAPGLVPPRGALGSEQQVRLDVVPLR
                     PRPGDLYVASTRQLREGELAALARDLASARDGAELLRAGVEGSSDRGRFTVRVLEGEE
                     DAASLPARAESLWRAPTAAEAAPEAKAGEPTLLEITPLIPESALVDAGAPVEETTASA
                     PERAGAAAPEPPVAPSIEDDEEERLGAPKFLLDPDAISDELAPSGVPGALRSAPVVED
                     DAAVAIPAVEGLEAGTIEAESVEAANAAPPPPRRLAQIDDAPPWYEPIALWVGGALAI
                     IAIALLVRAILPGLTGRHDKSAPLPPVVAAKGIVDFFSDPPGAAVRVDGDWLDGKTPL
                     VGVELETGAHRVELDWGPAGTWRDTVRVTGGERLAVHPAIYGTMALRASDPARTLDVY
                     LDGSFAGTTPLRLDRVPVGRHLVRFGGPGATGSTQEIDVLRDAPVEVIGSAGPVPTTG
                     KLTIRSALLTDAGFESGKGDPVWVDGVVRGVTPLSVSLKPGNHSVRVVRRNFPAQVTV
                     IDVKAGGDHYVTAEFGARSEQPLQLAPPEGISLSNPLPVTVTIPEDAWDPSVEVWLYA
                     ASPEGAFQAKRMTKLDSGERVFAGVVPTEVAQNGEHRVRIYCKASGTDGRELFTEIVT
                     LPVRP"
     gene            2852..4327
                     /locus_tag="VFM00_09575"
     CDS             2852..4327
                     /locus_tag="VFM00_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET7498537.1"
                     /translation="MIRFRSSRSFLAAIAFVALIAAYPVAPAFAGLASPDAPSWATAR
                     ADASDGPRVDDAKIAASDPLDVVRDRAAAAQDAVNSSLPAEALALVESSLRDLNAIVP
                     APQGAEALRDQLEGTRKRAQEQLDKAKEVVKQEDAKDGKPAADAPAAAVAKDPHKLNP
                     VVPETNERVEKWMTYYTGRGRDVFQKWLSRSGNYMDLLTQNLRAEGVPEELANLVFVE
                     SGFNMHARSVARAVGPWQFIRGTAKLFGLEITPYVDERRDPELATRAAAKYLRRLYEM
                     FDGSWPLALAAYNSGEGTVLRAIKRQKTNDFWSLNLPRETREYVPQFMAAMEIASDPE
                     RYGFTVPENSPWSVDAITVRGPIDLQLLSKVTEVPLDELERLNPAFVRHRSPADKEGT
                     MVRVPHGSGDQVQQIVDTKYHPRPLTRAEIRSAARAHRLELKHSKRYRYRRSGNLHVV
                     RRGETLSRIASRYGTSTTRLASLNGMPRNARIHAGQRIRVR"
     gene            complement(4367..5665)
                     /locus_tag="VFM00_09580"
     CDS             complement(4367..5665)
                     /locus_tag="VFM00_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase family
                     protein"
                     /protein_id="HET7498538.1"
                     /translation="MTAAVTYAMILAGGLGSRLCLLSEKRAKPAVPFGGKYRIIDFTL
                     SNAVNSGIFDIGILTQYRPLSLRDHIATGRPWDLDRKRGGVELLQPFQGWRENDWYRG
                     TADAVWQNWLEVEERGAEHLLVLSGDHVYRMDYRPLVEAHLARGAEATVATFEVPREQ
                     ASAFGVVRVDGAGWITGFLEKPKDPPSGKISMGVYVFHCGVLREALEKDAGRTSAHDF
                     GKDILPALVERGRVLAYPVPGYWQDIGTLDSYYQANLDLLSPEPAFPLEDPRWPIFTP
                     STENPPARLGPRAQVRASIVTHGTIVNGTVERSILFPGVVVEEGAVVRDSIVMGDSWI
                     GPRAVLDRAILDKRVHVGRGAVVGRGDVAPPNRVCPEHLSSGITIVGKHARVPEGVAI
                     GRNARIAPDVTEEDFPGGDVPSGEALERPGGVPAHAASGA"
     gene            complement(5662..6927)
                     /locus_tag="VFM00_09585"
     CDS             complement(5662..6927)
                     /locus_tag="VFM00_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET7498539.1"
                     /translation="MNPPILVLILAGARSDRLHALGRLRTASALPYGGKYRVIDFALS
                     NCVHSGLYRIGVLTQYAPLSLHGHIGIGRAWDLDRRDGGVRLLPAYARQRGTNWYRGT
                     ADALVQNRNVIEDAQARHILVLSGDLVAKINFGPILDRHEQRGAALTIVTVPAPSGAG
                     EWRRPVLSEPGGRVTAIPDRAADVGAPVSSSVFLFRARELRARLDRPDVGPDLMRDVV
                     EPMLAEGQPVYAHPFDGYWRQIASIDAYYEASMELLAPYPALNLHDPDWLIFTPSEDR
                     APAIVAGDGDPAGCLMAHGCRIEGTVRRSILFPGVHVAAGAVVEDSIVMHDARVLGGA
                     RVTRAIVDKGVRIGEGASVGGGEGPPNRDYPEDLAAGLALIGKGAEVPAGGRIGANTL
                     VDIGARESDFPARELGAGSVVRARGGRRA"
     gene            complement(6986..8017)
                     /locus_tag="VFM00_09590"
     CDS             complement(6986..8017)
                     /locus_tag="VFM00_09590"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET7498540.1"
                     /translation="MIRSPIRRPAFAAALLLLAAGLGAPAPARATRYAGEFLRIGVGA
                     RALGMGSAFMGLSDDGTAAYWNPAGLATLEHREITAMHAEQFGSIVQYDFLSYVMPIG
                     DPGRARQALGFSLVRLGVDQIPDTRGLEIIDQNGNGKFDYPEDRLVVDESRFVFDSDN
                     DVAFLLSYARELKRGFSVGGNFKVIRQWLGDSLRSTGFGLDGGALWVGPGGWSAGARL
                     SDATTTRILWNTGTNEFIAPALRIGAAKTKTFASRRHIVTAALDVQIGFSDEQLSSQA
                     HLGGVTFEFHPGVEYWIERRVALRAGFEARNFSAGAGLRIRRFGLDYAYLDHADLDAS
                     HRISGSYSF"
     gene            complement(8169..8591)
                     /locus_tag="VFM00_09595"
     CDS             complement(8169..8591)
                     /locus_tag="VFM00_09595"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7498541.1"
                     /translation="MTKPTRERRKSPRIAAKLAMQVSGGDESSVLTTESINLSASGIQ
                     FQSRAFLSPLTKVSLTILLPPFGRRLRRERIVKCDGIVVRCEEAEQAKRRPRYELACC
                     FLEVPDEERELLEQYVAWRAIRRVTLAEDAPRRRTAAS"
     gene            complement(8675..10666)
                     /locus_tag="VFM00_09600"
     CDS             complement(8675..10666)
                     /locus_tag="VFM00_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HET7498542.1"
                     /translation="MRRTLVGRLARTRFGYGFVRVDEEPEDLFIPPFAMGGALHGDRV
                     RAGYLETREQGDAHEVLEVLERTPYGIVGRMESRGRETALYPERPEYPHEIFLTLQGR
                     KSVPRGARVLVRLDPSPPDPITGRVVSVFEEDDPREDSLLVALEEGLKVEFEPEALEE
                     ASAHDEHSVARAARGREDFRDDLVVTIDPEDAKDHDDALSLRGDPERGPCHVGIHIAD
                     VTHYVRPGTALDREAQERGFSVYLADRTLPMLPDALSGGLCSLGDGVDRLTVSVVATL
                     APDGTLQGARIVEGVIRSAASLSYEEAGRLLREPGHGIPEALRGLDRLARALRKRRLE
                     EGGLEFDLPEIRPVLDASGEPVAFRERKQLPTHSLIEEFMLLANQVVGRRAAARDLPF
                     LYRVHERPRYDKLRSFVEAARYLGRSGPMPIVTEAKQLRHWASGGQTPRDRLVNLYLL
                     RALEKARYDLVDVGHFGLGMRGYAHFTSPIRRYPDLANHRIVKRYLADAAQGKTAPDP
                     WVFAAGWLNARTAAHSSEAEIAADEAERAVSRRKAVRFARARIGEEVGGYVSGLTPAG
                     VFVTLPEWNIDGFLPKRAMGDPSLALAEHGFSFRSKRSRRRFGLGDALRVVVARADLA
                     RREVELALAPPTGARARRTKHERKSGRMEAVGRRRKRRR"
     gene            complement(10926..11207)
                     /locus_tag="VFM00_09605"
     CDS             complement(10926..11207)
                     /locus_tag="VFM00_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7498543.1"
                     /translation="MTKADLVDEISGRTKISKSETATIVDQLLNAISRALSEGKHIEI
                     RGFGTFKVRERKARKARNPRSGAEVMVPAKLVPVFKASNELKGMVRGGD"
     gene            complement(11286..12173)
                     /gene="sppA"
                     /locus_tag="VFM00_09610"
     CDS             complement(11286..12173)
                     /gene="sppA"
                     /locus_tag="VFM00_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET7498544.1"
                     /translation="MARNRWVLGCALLLAGTVIIIGLAFMVVNLALTGDKMTLSPLSG
                     RVGLVEIVGEIDDAEPIVDQLDRMQRDSSVRAVVVRLDSPGGGVAASQEIYEAVKRVH
                     DEGKKPVIASMGGVAASGAYYVACAADSIVANAGTLTGSIGVIMSFPNTEDLFRKVGI
                     RFETIKTGKFKDIGSMWRPMTDDERKLLEGVLSNVYDQFVDAIVEGRSLAREDVLPYA
                     DGRIFSGDQAQEYGFVDRLGDLDDAVQLAGQMAGISGQPVVVRKERRRVSFWDFLDNK
                     MNQVTGLSSRGPRLEYRLR"
     gene            complement(12725..14269)
                     /gene="gpmI"
                     /locus_tag="VFM00_09615"
     CDS             complement(12725..14269)
                     /gene="gpmI"
                     /locus_tag="VFM00_09615"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET7498545.1"
                     /translation="MATKRPLTGLIVLDGWGLNPRTEGNAVRLARTPVMDRLLAEYPS
                     ATLTTCGEAVGLPKGQMGNSEVGHLNLGAGRIVYQDLTRIDRAMEDGSLAKNAVLQGA
                     FAHAREGDRSLHFIGLLSPGGVHSHERHLHAMIRAAAEARVPRIVVHAFLDGRDTAPR
                     SALPSVEATEALLRSLGPHGIATVAGRYYAMDRDKRWDRIERAYRALVAGNGERTATP
                     EEAVARAYERGEGDEFVAPSVVGDPNARRIARGDAVVGFNFRPDRMREISRALGDPAF
                     DAFPRPEWPLQLHYVCMTEYDETFPYPVLFKDEPLRKTLGEVVSAAGIRQLRIAETEK
                     YAHVTYFLNGSEEKPFPGEDRALIPSPKVATYDLKPEMSAVEVTDTLVRKLSDETYGF
                     FVLNYANADMVGHTGVIPAAVRAVETVDTGLGRVLEAVRARGGFALVTADHGNAEMMI
                     DPETGGPHTAHTLNPVPVLIAGDPRHALRDGILADVAPTLLHLLGIDAPAEMTGESLI
                     RTGQMD"
     gene            complement(14284..14727)
                     /locus_tag="VFM00_09620"
     CDS             complement(14284..14727)
                     /locus_tag="VFM00_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498546.1"
                     /translation="MRRAGFTAITVYALAMALVEAACVVSLKRLYFPEGWKPPFHAIP
                     VEGLLLEQWREVATLVMIAAVAFVGRPPWRIALARGLWIFGVWDIAYYGLLRWLTGFP
                     RTLGDFDVVFLVPRAWIAPVWLAVAGSAACLVAALALARGRRRTM"
     gene            complement(14724..15488)
                     /locus_tag="VFM00_09625"
     CDS             complement(14724..15488)
                     /locus_tag="VFM00_09625"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498547.1"
                     /translation="MPRPSGLLLPHLVLSGLEQMRLDESMLRWAALAPSRFAARTYAW
                     ARPTLSLGRAEPYPEGWNPEAIAAAGVDVVRRPTGGDAVLHDEEVTFAVAASIPGPWA
                     ERPRDFARLVAESLADALRGLGLPAAVVAPGEERLPAAAPGARLCFARAAAGEVRVAG
                     FKAAGIASRFTHGAALSHASVPLTARHRDAARFRRDAAADEAQVREHARSIGETLGRA
                     PEEGAVRDRLLQALAARFGVSLASGSLDDLAAGLAR"
     gene            complement(15496..16143)
                     /locus_tag="VFM00_09630"
     CDS             complement(15496..16143)
                     /locus_tag="VFM00_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498548.1"
                     /translation="MHLSTLPGVWISALLTLGIFSFLYKDNPVYKFCEHLFVGISAGY
                     WVVLTYYGTIKPNLVDPLFGVPFTFDRHLLLVMPLLLSLFLFSRFFPRGDWLSRWPIA
                     MVLGVYPALRITGFAQSDLVQQINGSILPFWVAGNAYASFNNILLVLGLLATLVFFFF
                     STEHKGAIGGIAKVGIYFLMVSFGASYGYTVMARVSLLIGRVMFLLDSWLGVVRF"
     gene            complement(16146..16976)
                     /locus_tag="VFM00_09635"
     CDS             complement(16146..16976)
                     /locus_tag="VFM00_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498549.1"
                     /translation="MSGLTALRKLDRRWIFLAVGLLVVVPLAVGIHIAPVQPSARARG
                     FYDAIEKVPAGSTVLLAGDYDPSTVAENYPMHLAAARHLMRRNIKIVGLALYPGGPPL
                     TDQVLRIAGGEYGKKEGVDYVNLGYKAGNELVMSQMGSSIPRTFPVDRRGIPVSQIPV
                     MRGVQNYANFPLLVNISAGYPGTKEWVQQVVSRYHLPMVAGVTAVSAPEYYPYLQAGQ
                     LKGLLGGMAGGAEYEVLIQKPALATRGMDAQSLAHLFIAFMILLGNLAFLGEDKERRR
                     "
     gene            complement(16973..17617)
                     /locus_tag="VFM00_09640"
     CDS             complement(16973..17617)
                     /locus_tag="VFM00_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498550.1"
                     /translation="MKRQVPFLITLLAGIFCLIGFFVPHPAIRTFYDEIQQWVIVIVG
                     ATYVLGVANLLRINLKQVSGRERDWPYKIVLLLGMVAMMIVGFTEGSHYLDPTSGFSW
                     MYNTFYSAMSATMFSLLAFFIASAAFRAFRIRTLEALLLAIAAFILMIGRVPIGNAIH
                     PFIPAAADWLMEVPQNAAKRGILIGAAIGVMATGIRLILGMERAYGSSSGGGGA"
     gene            complement(17628..18743)
                     /locus_tag="VFM00_09645"
     CDS             complement(17628..18743)
                     /locus_tag="VFM00_09645"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HET7498551.1"
                     /translation="MALAGAALALVVFAGFAAAFPLNAINTPNDRGQSVTLTWTSPTP
                     APAHWILLRRERGGTFAPVDTLDGAMKTHTDEGEGAQGVQDGTTYEYRLIADPPNPAL
                     NSDISAAATPGPEWIDSTKVNVLVVTLLFFIVLFYFINQAHRGVRWKFRTIPGLIAIE
                     EAIGRATEMGKGVLYIPGVQDIDDIQTIASLIILRNVARTTAKYETRLTVPTNAPAVY
                     TVADEVVKSAYQDVGRADAYQSDQVRYVTSEQFAYVASVNGMMLRERPAANLLLGAFF
                     AESLLLAETGHAIGAIQIAGTANVHQMPFFVVACDYTLIGEEYYAASALLSNEPLLLG
                     SLKSADIVKLVLIVVIIIGCAMATFGNPALGLWFGTE"
     gene            complement(18949..20079)
                     /locus_tag="VFM00_09650"
     CDS             complement(18949..20079)
                     /locus_tag="VFM00_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498552.1"
                     /translation="MAHSYTPGLKVTAYAVVRRERKLPLAGTVLVKVGDAVKAETVVA
                     RTELPGNVQTVNAANILGVLPEDVTECLVKPVGSAVAKGEPFAESRSFFGLFRSKVPS
                     PIDGTLESVSTVTGQALLREAPIPVEVDAYVDGTIVEVHPNQGVTVETRGTFIQGIFG
                     IGGETSGVLRVVAGSPGETLKSAAITEAHRGCVVVGGAHVTTATLRHAVAMGVKAIVI
                     GGFDDQDLREFLGYDLGVAITGSEEKGLTLVVTEGFGEIPMANRTFELLKQCEGRKVS
                     VSGATQIRAGVLRPEIIAPRLESAAPQPGSGDGAHEGGLEVGSLIRVIRQPYFGRLGK
                     VTALPAELTALETEAKVRVLEVDFGNGSRTMLPRANVEMIES"
     gene            complement(20131..21990)
                     /locus_tag="VFM00_09655"
     CDS             complement(20131..21990)
                     /locus_tag="VFM00_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HET7498553.1"
                     /translation="MPVPAESLRSILATDCGSTTTKAILIERRGDEYRLIVRGEAPTT
                     VEAPFEDVTRGVLNAIMEVEELAGRKILDGERIITPQQGNEGVDLYISTSSAGGGLQM
                     MVSGVVKSMTGESAQRAALGAGAIVMDVIASNDGRLPHEKIKRIRQLRPDMILLSGGV
                     DGGTTSHVAEMAELLAAAEPKARLGAGYELPVIYAGNKDAAQIVRDRLEDKTALVITD
                     NLRPVLERENLTPARDKIHDLFMEHVMAHAPGYKKLMTWSPIPIMPTPGAVGLIIETI
                     ARDEKINVIGVDIGGATTDVFSVFQGVFNRTVSANLGMSYSVSNVLAEAGLPNIMRWV
                     PFAIDELDLRNRIKNKMIRPTTIPQSLEELVIEQAISREALRLAFEQHKQLAVGLKGV
                     QQERTISDTFEQTASGETLVRLDHLDLLVGSGGVLSHAPRRSQAALMMLDAFLPEHVT
                     ELAVDSIFMAPQLGVLSTVHPVAARQVFEKDCLIRLGTSVAPVGPGKEGQTMLRVSLE
                     LPGGKKEEFPVPYGYLKLVPLADGERAKATLRPEKSFDVGEGKGRERTVTLRGGVVGL
                     IFDCRGRRPFGLPEDRARRIAKLAEWNQALDIYPASKAALAGAGAPAAVGAGA"
     gene            complement(22026..22418)
                     /locus_tag="VFM00_09660"
     CDS             complement(22026..22418)
                     /locus_tag="VFM00_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498554.1"
                     /translation="MGWWRSFKHASRAPGAGEALTDEDRRLLDRLAETTVRYRMTVPA
                     ILFLESMKPLSFVGSQAMYFFEPMVRALFAVPEYERFAALMERRESVEALLVAIERRD
                     DEERRSEKEKKQRERDERAALRKRKESE"
     gene            complement(22366..22881)
                     /locus_tag="VFM00_09665"
     CDS             complement(22366..22881)
                     /locus_tag="VFM00_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498555.1"
                     /translation="MARERPAAAARLGNAGAEEAEAVSEHRGTAPEADGAELSWSVWP
                     ARQKPLTAVVIVAGAVVLGVLIARGTGDRLLGIAAPIFVLLSLSSYLLPTRYRLTPDA
                     VEVRSLGVVRARPWSEMKRFEEDEAGLFLSPFEKRSWLDAYRGVRLPLGGNRDGVVAF
                     VQARVARAGRR"
     gene            complement(22871..24337)
                     /gene="gcvPB"
                     /locus_tag="VFM00_09670"
     CDS             complement(22871..24337)
                     /gene="gcvPB"
                     /locus_tag="VFM00_09670"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET7498556.1"
                     /translation="MSELTLHEGSVKGHRGPRLAPLDVPEQGAARLLPGVRLRPAAPD
                     LPQISEPAVVRHFVNLSVMNHHIDRAIYPLGSCTMKYNPKLNDEMARIPGFSAIHPLQ
                     PEETAQGALAVIGELHEWIRAIMEMPGVTTQPAAGAQCELTAILMTRAYHAERGDARE
                     EVIVPDSAHGTNPSSVAQVGYKVVTVPSGPDGCVDLEALRAAVSPRTAALMLTNPNTL
                     GIFEHNVGEIARIVHDAGGLLYLDGANLNAMVGLASPGRMGFDFAHINLHKTFSTPHG
                     GGGPGGGVLCVRAELEPYLPAPVLVRQADCWHWDEDRPRSIGRVHSFYGNFGILVRAL
                     TYMRSLGASGIAEVSREAILNANYLKARLIEAYEPSHAGYCMHEFVLSASRQKARGAR
                     ALDVAKRLLDFGVHAPTTYFPLIVDEALMVEPTETETKASLDAFAEAMLAIDRESIEA
                     PQKLKDAPTRTPVRRLDEARAARQLVLSWQDAEAAGGA"
     gene            complement(24325..>24606)
                     /locus_tag="VFM00_09675"
     CDS             complement(24325..>24606)
                     /locus_tag="VFM00_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498557.1"
                     /translation="AVPGVTLAHGGAPFFREFVVRLPGSAERFVHAALGSRILAGVPL
                     SRFDADRPADLLVAATEKRSKEEMDRYVETLARWARAGRPAPEEAVCPS"
BASE COUNT         3750 a         8663 c         8608 g         3585 t
ORIGIN      
        1 gacgcgcgac gccggcgcgg cgcgagcccg cggcgccggc gagcagcgag accgcaccga
       61 gcccggcaca cgcggcgagc gcgtgcatgc gcagggccgc gggcacgggc gcggcccagc
      121 cggccgcgtg tccggcggca agcagcatcc aggcgagcgc ggaagcggga agacggaacg
      181 aagcgagcgg gcgcacgtgc gcctccggcg cggggcggtg gagcgacgcg cggaggaggt
      241 cgcgcgatgt cgagagcggc gccaaccacg aggcgccgcg gcgttcggga gcggcgcgaa
      301 ccacggcgcc gcggttcttc ctaggggcgg acggggaggg tgacgatctc cgtgaagagc
      361 tcgcgtccgt cggtgccgga cgccttgcag tagatacgga cgcggtgctc gccgttctgc
      421 gccacttcgg tcggcacgac cccggcgaac acgcgttcgc cgctgtcgag cttggtcatg
      481 cgcttcgcct ggaaggctcc ctcgggggaa gcggcgtaga gccagacctc gaccgaggga
      541 tcccaagcat cctcggggat cgtcacggta acggggagcg gattggatag cgagatgcct
      601 tccggcggcg ccagctggag cggctgctcg gagcgggcgc cgaactccgc ggtgacgtag
      661 tgatcgccac ccgccttcac gtcgatcacg gtgacctggg cgggaaagtt gcgccggacg
      721 acccgcacgc tgtgattgcc cggcttgagc gagacggaga gcggcgtcac gccacgcacc
      781 acgccgtcga cccagacggg atcacccttc cccgactcga acccggcgtc ggtgagaagg
      841 gccgagcgga tggtgagctt tccggtcgtg ggcacggggc ccgcgcttcc gatcacctcc
      901 acgggcgcgt cgcgcagaac atcgatctcc tgcgtcgagc cggtcgcgcc ggggccgccg
      961 aagcgcacca ggtggcgccc cacgggcacg cggtccaggc ggagcggtgt cgttcccgcg
     1021 aacgatccgt cgagatagac gtccagcgtg cgcgccggat cggaggcgcg gagcgccatg
     1081 gttccgtaga tcgcgggatg gacggcgagc cgctcgcccc cggtcacccg aaccgtgtcg
     1141 cgccacgtgc cggcggggcc ccagtcgagc tcgacgcgat gcgcgcccgt ctccagctcc
     1201 acgccgacga gcggcgtctt cccgtcgagc caatcgccgt ccacgcgcac cgcggctccg
     1261 ggagggtcgg agaagaagtc gacgatcccc ttcgcggcca cgacgggcgg aagcggcgcg
     1321 ctcttgtcgt gtcggccggt gaggcccgga aggatggcgc gcacgaggag tgctatggcg
     1381 atgatcgcga gcgctccacc gacccagagc gcgatcggct cgtaccaggg aggcgcgtcg
     1441 tcgatctgcg cgagccggcg cggcggcgga ggcgcggcgt ttgccgcttc gaccgattcg
     1501 gcctcgatcg tccccgcttc gagaccttcc acggcaggga tggccacggc agcgtcatcc
     1561 tccaccaccg gcgccgagcg cagcgcgccc ggaacgcccg atggcgcgag ctcgtcggag
     1621 atggcgtcgg ggtcgagcag gaatttcggc gcgccaaggc gctcttcctc atcgtcctcg
     1681 atcgaaggcg cgacgggtgg ctccggtgcc gcggcgcccg cgcgctcggg cgcggatgcc
     1741 gtcgtctcct cgacgggagc gccggcgtcg accagggcgc tttcggggat caggggcgtg
     1801 atctcgagca gcgtgggctc gcccgccttc gcctcaggcg ccgcctcggc cgcggtcggg
     1861 gcgcgccaga gcgattccgc tcgcgccgga agcgacgccg cgtcctcttc accttccagg
     1921 acgcggacgg tgaagcggcc gcggtcgctc gatccctcga cgcccgcccg gagcagctcg
     1981 gcgccgtcgc gcgcggaggc gaggtcccgg gccagggcgg ccagctcccc ttcgcgcagc
     2041 tgtcgcgtgg aggccacata gaggtccccg ggccgcgggc gcagcggaac cacgtccagg
     2101 cgcacctgct gctcggaacc gagggcgccc cggggcggga cgagtcccgg ggcgtctccg
     2161 gggcgctcca ggcgcgacag gaggccgtcc cggaagcgga aacaggcgca atcccccgcc
     2221 gagaccacga agccgtcccc ggcgtggaag acggcggcca ggaccgacac ccaagggcgg
     2281 tcccgggccg ggcggcggac caattcagcg tggatcgacc ggagggcggt gatgatgcgc
     2341 cccagggggg tctccgaggg gtcggatcca gccaccaggg ccacggatag cttctctttg
     2401 acccagatgg ttgggtcaac gacatcagtc agggaggcgg ccccccgggc caccagggcg
     2461 aagccccggt ccagatcgag gtgggccaga gccccttcgt ggtcagggct ccgcaccagt
     2521 cccgcgactc gaagctgggt gccgagttcc gcgttcatcg aatccgcaca ataggggcgg
     2581 catccgaaca aagtcaatga tgggcttgtc ccattgctgg cacgtcagtt gcagtgttcc
     2641 aactcaggca aggaccaccg ccaaaaccaa gaggattcgg gctggaaaac ggcacgaccg
     2701 ggcagacgcg acttgcgttc tgcacgggga gcggctaacg tgtcgaggtt cctgccggca
     2761 ctaaatcgaa ccctcggatg tttgttgacc aacgatcgga ccgctcgacc aacgtggcct
     2821 cgagtccggt cgcaccctac gggaggcccc gttgattcga tttcgatcga gtcggtcgtt
     2881 cctcgcggca atcgccttcg tcgccctgat cgcggcgtat ccggtcgcgc ccgccttcgc
     2941 cgggctcgcc agccctgacg ccccgagctg ggccacggcc cgtgccgacg cctcggacgg
     3001 cccccgggtc gacgacgcca agatcgccgc ttccgacccc ctcgacgtcg tccgcgaccg
     3061 ggctgccgcc gcccaggacg ccgtgaactc cagcctcccg gccgaggcgc tcgccctcgt
     3121 cgaatcctcc ctccgcgacc tgaacgcgat cgtccccgcc ccccagggcg ccgaggcgct
     3181 gcgcgaccag ctcgagggga cgcgcaagcg cgcgcaggag cagctcgaca aggccaagga
     3241 agtggtgaag caggaggacg cgaaggacgg caagcccgcc gccgacgccc ccgccgcagc
     3301 cgtcgcgaag gatccccaca agctgaatcc ggtcgtcccg gagacgaacg agcgggtcga
     3361 gaagtggatg acctactaca ccggccgcgg ccgtgacgtc ttccagaagt ggctctcgcg
     3421 ctccggcaac tacatggacc tcctcacgca gaacctgcgc gccgagggcg tccccgagga
     3481 gctcgcgaac ctcgtcttcg tcgagagcgg cttcaacatg cacgcccgct ccgtggcgcg
     3541 cgccgtgggt ccctggcagt tcatccgcgg caccgcgaag ctcttcggcc tggagatcac
     3601 gccctacgtc gacgagcggc gcgaccccga actggcgacg cgcgcggccg cgaagtacct
     3661 gcgccgcctc tacgagatgt tcgacggctc ctggccgctg gcgctggccg cctacaacag
     3721 cggcgagggg acggtgctcc gcgcgatcaa gcgccagaag acgaacgatt tctggtcgct
     3781 caacctgccg cgcgagacgc gcgagtacgt gccgcagttc atggccgcca tggagatcgc
     3841 ctccgatccc gagcgctacg gcttcacggt gcccgagaat tcgccctgga gcgtcgacgc
     3901 gatcaccgtg cgcgggccga tcgacctgca gctcctctcc aaggtcacgg aagtcccgct
     3961 cgatgagctg gagcgcctga acccggcgtt cgtccgccac cgctcccccg ccgacaagga
     4021 ggggacgatg gtccgcgtgc cgcacgggag cggcgaccag gtccagcaga tcgtggacac
     4081 gaagtaccac ccgcggccgc tcacgcgcgc cgagatccgc tccgcggcgc gcgcgcaccg
     4141 gctggagctg aagcactcga agcggtaccg ctaccgccgc tccggaaacc tgcacgtggt
     4201 ccgccgcggc gagacgctgt cgcgcatcgc ttcgcgctac ggcaccagca ccacccggct
     4261 cgcgagcctg aacggcatgc cgcgcaacgc gcggatccac gccgggcagc gcatccgcgt
     4321 ccgctgacct tccccgtacc ggaatcgagg gtgattcgcg cctgagttag gcgcctgatg
     4381 ccgcgtgcgc cgggacgccg cccggccgct ccagcgcttc tcccgaggga acgtcgcctc
     4441 ccggaaaatc ctcctcggtc acgtccgggg cgatccgcgc gttccggccg atcgcgaccc
     4501 cttcggggac ccgcgcgtgc ttccccacga tcgtgatgcc cgaggagagg tgctccggac
     4561 agacgcgatt gggcggagcg acgtcgccgc gcccgaccac ggctcccctt cccacgtgga
     4621 cgcgcttgtc gaggatggcg cgatccagca cggcgcgcgg cccgatccag gaatctccca
     4681 tcacgatcga atcgcgcacg acggccccct cctccacgac aacgccggga aagaggatgg
     4741 agcgctcgac cgtgccgttc acgatcgtcc cgtgcgtcac gatggaggcg cgcacctgcg
     4801 cgcggggtcc gagccgcgct ggcggattct cggtgctcgg cgtgaagatc ggccagcgcg
     4861 ggtcctcgag cggaaacgcg ggctcgggcg agagcaggtc gaggttggcc tggtaataag
     4921 aatccagcgt gccgatgtcc tgccagtagc ccggcacggg ataggcgagg acccggccgc
     4981 gctcgaccag ggcgggaagg atgtccttgc cgaagtcgtg cgcgctggtc cggccggcgt
     5041 ccttctccag cgcctcgcgc agcacgccgc agtggaagac gtagaccccc atcgagatct
     5101 ttcccgacgg gggatccttg ggcttctcga gaaagccggt gatccatccg gctccgtcga
     5161 cgcgaacgac gccgaaggcg gaggcctgct cgcgcggcac ctcgaacgtg gcgaccgtcg
     5221 cctcggcgcc gcgggcgaga tgcgcctcga cgagggggcg atagtccatg cgatagacat
     5281 ggtcgccgga gaggaccagg agatgctccg cgccgcgctc ctcgacctcc agccagttct
     5341 gccagaccgc gtcggcggta ccccggtacc agtcgttctc gcgccagccc tggaagggct
     5401 gcaggagctc gaccccgccg cgcttgcggt cgagatccca cggccggccc gtcgcgatgt
     5461 ggtcgcgcag ggagagcggg cgatactgcg tgaggattcc gatgtcgaag atcccggagt
     5521 tcaccgcgtt ggagagggtg aagtcgatga tccggtactt gccgccgaac ggcaccgcgg
     5581 gcttcgcgcg cttctcggag aggaggcaga gccggctccc caggcctccc gcgaggatca
     5641 tggcgtaggt gacggccgcg gtcacgcgcg cctcccgccg cgcgcgcgga ccaccgagcc
     5701 ggcgcccagc tcgcgcgccg ggaaatcgga ctcccgcgcc ccgatgtcca cgagcgtgtt
     5761 cgcgccgatg cgcccgccgg cggggacctc ggcgcccttg ccgatcaggg cgaggcccgc
     5821 cgcgagatcc tcggggtagt cccggttggg cggtccttcg ccgccgccca ccgaagcgcc
     5881 ctcgccgatc cgcaccccct tgtccacgat ggcgcgcgtc acacgcgccc ctccgagcac
     5941 gcgcgcgtcg tgcatcacga tcgagtcctc cacgacggcc cccgcagcca cgtgcacgcc
     6001 cggaaagagg atcgagcggc gcacggtccc ctcgatcctg cagccgtgcg ccatgaggca
     6061 ccccgccgga tcgccgtccc cggccacgat ggcgggcgcg cggtcctcgc tcggcgtgaa
     6121 gatcagccag tcggggtcgt ggaggttcag cgccgggtag ggcgccagga gctccatgct
     6181 cgcctcgtaa taggcgtcga tggaggcgat ctggcgccag tagccgtcga agggatgcgc
     6241 gtagaccggc tgtccctcgg cgagcatcgg ctcgacgacg tcgcgcatca ggtcggggcc
     6301 gacgtcgggg cggtcgaggc gcgcgcgcag ctcgcgcgcg cgaaagagga agacggagga
     6361 ggaaacgggt gcgcccacgt ccgccgcgcg gtcggggatg gccgtcacgc ggccccccgg
     6421 ctccgacagc acgggccggc gccactcgcc ggcgccgctc ggcgccggca cggtcacgat
     6481 cgtgagcgcg gcaccccgct gctcgtgccg gtcgaggatc ggcccgaagt tgatcttcgc
     6541 gacgagatcg ccggagagga ccaggatgtg gcgcgcctgg gcgtcttcga tgacgttgcg
     6601 gttctgcacg agcgcgtcgg cggtgccgcg gtaccagttg gtcccccgct gccgcgcgta
     6661 ggcgggcagg aggcgcacgc cgccgtcgcg ccggtcgaga tcccacgcgc ggccgatgcc
     6721 gatgtggcca tggagggaga gcggcgcgta ctgcgtgagc acgccgatgc ggtagagccc
     6781 cgagtggacg cagttggaga gggcgaagtc gatcacgcgg tacttcccgc cgtaggggag
     6841 cgcggaggcg gtccggaggc ggcccagcgc gtgcagccgg tcgctgcgcg cgcccgcgag
     6901 gatcaggacg aggatgggag ggttcaccac gtcgcgacct ccggggttcc agagagagcc
     6961 cgggcgtcaa cccccggcgc ggccgtcaga aggagtagga gcccgagatc cggtggctgg
     7021 cgtcgaggtc ggcgtgatcc agatacgcgt aatccagccc gaagcggcgg atgcggagcc
     7081 ccgccccggc cgagaaattc cgcgcctcga acccggcgcg gagggccacg cgccgctcga
     7141 tccaatactc cacgcccgga tggaattcga aggtgacgcc gccgagatgc gcctggctgg
     7201 agagttgttc gtccgagaag ccgatctgca cgtcgagcgc ggcggtcacg atgtgacgcc
     7261 gcgacgcgaa cgtcttcgtc ttggccgcgc cgatccgcag cgccggcgcg atgaattcgt
     7321 tggtgccggt gttccagagg atgcgcgtgg tcgtcgcgtc ggagagacgc gcgcccgcgg
     7381 accatccgcc ggggcccacc cagagcgcgc cgccgtcgag gccgaatccg gtcgagcgga
     7441 ggctgtcccc cagccactgc cggatgacct tgaagttgcc tcccacggag aatccgcgct
     7501 tcagctcccg cgcgtacgag agcaggaagg cgacgtcgtt gtcgctgtcg aagacgaacc
     7561 ggctctcgtc caccacgaga cggtcctcgg ggtagtcgaa cttgccgttt ccgttctggt
     7621 cgatgatctc gagtccgcgc gtgtcgggga tctggtccac ccccagccgg acgagcgaga
     7681 agccgagcgc ctgccgggcg cgcccgggat cgccgatcgg catcacgtag gagaggaagt
     7741 cgtactgcac gatggagccg aactgctcgg cgtgcatcgc ggtgatctcg cggtgctcca
     7801 gcgtggcgag cccggcggga ttccagtagg ccgcggtccc gtcgtcggag agtcccatga
     7861 aggcgctccc catcccgagg gcgcgcgcgc cgacgccgat gcggaggaac tccccggcat
     7921 agcgcgtggc ccgcgccggg gcgggcgcgc cgaggccggc cgcgagcagg aggagggcgg
     7981 cggcgaacgc cgggcgacgg atcgggcttc gaatcatcgg tcctcgtatc gggtgcgctg
     8041 ggaactcccc ggagcctcgg gccaggggcg ttcagatgcc tccggaccgg gagggtcacc
     8101 gggtgctacc ccccagccga tgccagggtt ccccttcggg acaccggcag ggccgtggcc
     8161 gcggaagatc aggaggccgc cgtccggcgc cgcggggcgt cctcggccag cgtgaccctc
     8221 cggatcgccc tccaggccac gtactgctcc aggagctccc gttcctcgtc ggggacctcg
     8281 aggaagcagc aggccagctc gtagcggggc cgccgcttgg cctgctccgc ctcttcgcag
     8341 cggacgacga tcccgtcgca cttcacgatc cgttcccgcc gcagccggcg cccgaacggc
     8401 gggagcagga tggtgagcga gaccttggtg aggggcgaca ggaacgcgcg gctctggaac
     8461 tggatgccgc tcgcggaaag attgatgctc tccgtggtca ggaccgagct ctcgtctccg
     8521 cccgagacct gcatggcgag cttcgcggcg atgcgcggcg acttccgtcg ctcgcgggtc
     8581 ggcttggtca tcggcactcc tcgcgtggcg cttctcccac ttcccccggc gccccctcga
     8641 ggcgcccctc ccgcgggccc gaaggcgccg cacctcaccg gcgccgcttc cggcgccgcc
     8701 ccaccgcttc catccgcccg gacttccgct cgtgcttggt ccggcgcgct cgcgcccccg
     8761 tcgggggcgc gagcgcgagc tccacttcgc gccgcgcgag gtcggcgcgg gccacgacga
     8821 cgcgcagcgc gtcccccagc ccgaaccgcc ggcgcgaccg tttcgagcgg aacgagaatc
     8881 cgtgctcggc gagcgcgagg ctcggatcgc ccatcgcgcg cttcggcagg aatccgtcga
     8941 tgttccactc cgggagcgtg acgaacaccc ccgccggcgt gagcccggag acgtaccctc
     9001 cgacctcctc gccgatccgc gcccgcgcga agcggaccgc cttccggcgg ctcaccgcgc
     9061 gctccgcctc gtccgcggcg atctccgcct ctgacgagtg cgctgccgtc ctggcgttga
     9121 gccacccggc cgcgaacacc cacgggtcgg gcgccgtctt gccctgcgcc gcgtcggcca
     9181 gatagcgctt cacgatccga tgattcgcga gatcgggata gcgccggatg ggcgacgtga
     9241 aatgggcgta gccgcgcatg cccagcccga aatggccgac gtccacgagg tcgtaacgcg
     9301 ctttctcgag cgcgcgcagc aggtacagat tcacgaggcg gtcccgcggc gtttgtccgc
     9361 cgctcgccca gtgacggagc tgcttggcct cggtgacgat gggcatcggg ccggaacgcc
     9421 ccagatagcg cgcggcctcg acgaacgagc gcagcttatc atagcggggt cgctcgtgga
     9481 cccggtaaag gaacgggaga tcgcgagccg cggcgcgccg ccccaccacc tggttcgcga
     9541 gcagcatgaa ctcctcgatg agcgagtgcg tcggcagctg tttccgttcg cggaaagcga
     9601 ccggctcccc cgaagcgtcg agcacgggac ggatctccgg cagatcgaac tccagtcccc
     9661 cctcttccag acgccgcttc cgaagcgcgc gcgccaggcg gtcgaggccg cgcaacgctt
     9721 cgggaattcc atgcccgggc tcgcgcagga gccgccccgc ctcttcgtag gagagcgacg
     9781 ccgcgctccg gatcaccccc tccacgatgc gcgcgccctg gagcgtgccg tccggagcga
     9841 gcgtggcgac gacgctcacc gtgaggcggt cgaccccgtc tcccaggctg cagaggccgc
     9901 ccgagagcgc gtccgggagc atgggcaggg tgcggtcggc gaggtacacg ctgaagccgc
     9961 gctcctgcgc ctcgcggtcg agggccgtgc cgggacgcac gtagtgggtc acgtccgcga
    10021 tgtggatgcc gacatggcag gggccgcgct ccgggtcgcc gcgcagcgaa agggcgtcgt
    10081 cgtggtcctt ggcgtcttcc gggtcgatcg tgaccaccag gtcgtcccgg aagtcctccc
    10141 ggccgcgcgc ggcgcgcgcc acggagtgct cgtcgtgcgc cgaggcctcc tccagcgcct
    10201 cgggctcgaa ctccaccttg agcccctctt ccagcgccac gagcaggctg tcttcgcggg
    10261 gatcgtcctc ctcgaagacc gagacgacgc ggccggtgat cggatcggga ggcgacggat
    10321 ccaggcggac caggacccgc gcgccgcgcg ggacgctctt ccggccctga agggtgagga
    10381 agatctcgtg cgggtattcg ggtcgttcgg ggtagagggc cgtttcgcgt ccccgggact
    10441 ccatccggcc gacgatgccg tagggggtcc gctcgagaac ctcgagcacc tcgtgcgcgt
    10501 ccccctgctc gcgggtctcc aggtagccgg cccgcacccg gtccccatgg agcgccccgc
    10561 ccatggcgaa ggggggaatg aagaggtcct cgggctcctc gtcgacgcgc acgaacccgt
    10621 agccgaagcg cgtgcgcgca aggcgcccga ccagcgtgcg gcgcatcagc gatccggagt
    10681 gctcatgacc ctgtcggcca agggggcgtc atagaaccgg cccgggcatc ggggaccgat
    10741 gcgcgccggg ccgggtaccg atagtgcggc gcgggagcgg caccgcgatc ctgagcgacc
    10801 tgcgctcagc gaagacgttt cttgtgccga tcgcggcgaa gacgcttttt ccgtttgtgc
    10861 gtggcgatct tctttcgttt ccgcttcttt ccgcttccca tcggtctcct tcggccggcg
    10921 cggggtcagt ccccgccccg aaccatccct ttgagctcgt tggacgcctt gaagacgggc
    10981 accagcttcg ccggtaccat gacctcggcg ccgcttcgtg ggttgcgcgc ttttcgcgcc
    11041 ttgcgttcgc gcaccttgaa ggtgccgaaa ccgcgaatct cgatgtgctt tccttcgctc
    11101 agggcccgcg agatcgcgtt cagcagctga tcgacgatcg tggccgtctc gctcttggaa
    11161 atcttcgttc gacccgaaat ctcgtcgacc aggtcggctt tggtcatggg aaatcctccc
    11221 tcgaggcgcc ggcccactgg ccggccagcg cgtcgcaatc tagcatctgc ccttcggcgc
    11281 gtcaattacc ggaggcggta ctccaggcga ggtccgcggg aggagagccc cgtgacctgg
    11341 ttcatcttgt tgtcgaggaa gtcccagaac gagacgcggc ggcgctcctt gcggaccacg
    11401 accggctggc cgctgatccc ggccatctgg ccggccagct ggacggcatc gtccaggtcc
    11461 cccagccggt ccacgaatcc gtattcctgc gcctggtcgc cgctgaagat gcgtccgtcc
    11521 gcgtacggca gcacatcctc gcgcgcgagg ctccgtccct ccacgatcgc gtccacgaac
    11581 tggtcgtaga cgttggagag aacgccctcg agaagcttcc gctcgtcgtc ggtcatcggc
    11641 cgccacatgg agccgatatc cttgaacttg ccggtcttga tcgtctcgaa gcggatgccg
    11701 accttccgga acagatcctc cgtgttcggg aagctcatga tcacgccgat gctgccggtc
    11761 aaggtcccgg cgttggccac gatcgagtcg gcggcacagg cgacgtagta ggcgccggag
    11821 gcggccacgc cgcccatcga cgcgatgacc ggcttcttcc cttcgtcatg cacccgcttt
    11881 accgcctcgt agatctcctg cgaggcggcc acgccgccgc ccgggctgtc gaggcgcacg
    11941 accacggcgc gcacgctgga gtccctctgc atccggtcca gctggtccac gatcggttcg
    12001 gcgtcgtcga tctcgccgac gatctccacg agcccgaccc ggccggagag cggcgagagc
    12061 gtcatcttgt ccccggtcag cgccaggttc accaccatga acgcgaggcc gatgatgatg
    12121 acggttccgg caagcaggag ggcacaaccg aggacccagc ggtttcgcgc cacgacgttc
    12181 tcccgggtga gcggcggatg taaaccgtgt caccctaaca gaatcaccga cgcgattcca
    12241 atgcaggaag gcgcgcggcc tgttccgccg cgcgcctcct tcggcctttc ggccttggtt
    12301 attgctcccc gcctgtgccg tgcacccagt gccggtgaaa ctgcgtcgtc tcgtcgaggg
    12361 gatcagctca ggaccgggcg attctcgcga gcacggcagg gaacatcggg tttgtctggt
    12421 tcgttcctcc aaggttcgct tcgtttccgt tcgctgcgtc cgacgctatg gactaaggca
    12481 atcggtgtgc cgcgcgggtt ggatggggct tggtggaggg gttggtggtg gctcgatcga
    12541 gccaatggta agctgatgca ctacagcgta ttaggggcgg tactttaggc tatttcgcag
    12601 gggatctgga gaggctagga ggtcatgtac tcagaggagg gcaagtctca gatttctggg
    12661 gtgccgcccg cagaacctgg tgcgaaaaga cccagagttc cgcggcctgc gggccgcggt
    12721 acatctaatc catctgcccc gttcgtatca ggctctcacc cgtcatctct gccggcgcgt
    12781 cgatgcccag caggtgcagc agcgtcgggg cgacgtcggc caggatgccg tccctcagcg
    12841 cgtggcgcgg atcgccggcg atgagcaccg ggacgggatt cagggtgtgc gcggtatggg
    12901 ggccgcccgt ttcggggtcg atcatcatct cggcgttgcc gtggtccgcc gtgaccaggg
    12961 cgaatccacc gcgcgcgcgg accgcctcca gcacccggcc cagacccgtg tccaccgtct
    13021 ccacggcgcg gaccgcggcc ggaatgacgc ccgtatgccc caccatgtcg gcattcgcgt
    13081 agttgaggac gaagaagccg taggtctcgt ccgagagctt gcgcaccagg gtatcggtca
    13141 cctcgaccgc gctcatctcc ggcttcagat cgtaggtggc caccttgggt gaggggatca
    13201 gggcgcggtc ctcccctggg aacggcttct cctcgctccc gttcaggaaa taggtcacgt
    13261 gcgcgtactt ttccgtctcc gcgatccgga gctggcggat cccggcggcg ctcaccacct
    13321 cgcccagggt cttccggagc ggctcatcct tgaagagcac cggatacgga aacgtctcgt
    13381 cgtactcagt catgcagacg taatggagct ggagcggcca ctccggccgc gggaaggcgt
    13441 cgaaggcggg atcgcccagg gcccgggaga tctcgcgcat gcggtccgga cggaagttga
    13501 agccgaccac ggcgtctccc cgcgcgatgc gccgcgcgtt tggatcgccg acgaccgagg
    13561 gcgccacgaa ctcgtccccc tcgccgcgct cgtacgcgcg cgcgaccgcc tcctcggggg
    13621 tcgccgtccg ctccccgttc cccgccacga gcgcgcggta ggcccgctcg atccggtccc
    13681 accgcttgtc gcgatccatg gcgtagtagc ggccggccac cgtggcgatc ccgtgcgggc
    13741 cgagcgagcg gagaagcgcc tcggtcgctt cgaccgaagg gagcgccgag cggggcgccg
    13801 tatcgcggcc gtcgaggaag gcgtgcacga cgatccgcgg cactcgggct tcggcggcgg
    13861 cgcggatcat cgcgtgcagg tgccgctcgt ggctgtggac gccgcccgga gaaaggagcc
    13921 cgatgaagtg gagtgagcgg tccccttccc gggcgtgcgc gaacgctccc tggaggacgg
    13981 cgttcttcgc gagcgatccg tcctccatgg cgcggtcgat gcgcgtgagg tcctggtaga
    14041 cgatccgccc ggcgcccagg ttcaggtgcc ccacctcgga gttccccatc tgtcccttcg
    14101 ggaggccgac ggcttcgccg caggtggtga gcgtggcgct ggggtattcg gcgaggaggc
    14161 ggtccatgac cggagtgcgc gccaggcgca cggcgttccc ctcggtcctg gggttcagtc
    14221 cccagccgtc gagcacgatc agtccggtga gcggccgctt cgtcgccatg gccgaacagc
    14281 ctatcacatc gtgcggcggc ggccccgggc gagcgccagt gcggccacga ggcaggcggc
    14341 cgagcctgcc acggccagcc agaccggagc aatccacgcg cgcggcacca ggaacacgac
    14401 gtcgaaatcg ccgagcgtac gggggaaacc ggtcagccat cggaggagcc cgtagtacgc
    14461 gatatcccag acgccgaaga tccagagccc ccgcgccagc gcgatcctcc acggcggtct
    14521 ccccacgaac gccacggccg cgatcatcac gagggtcgcg acctcgcgcc actgctccag
    14581 caggaggccc tcgaccggga tggcgtggaa cggcggcttc cacccctcgg gaaagtagag
    14641 gcgcttcagg gagacgacgc acgccgcctc gaccagcgcc atcgcgagcg cgtagacggt
    14701 gatcgcggtg aagccggccc gcctcaccgc gccaaccccg cggccaggtc atccaggctc
    14761 cccgacgcga gcgaaacgcc gaagcgcgcc gccagcgcct ggaggaggcg gtcccggacg
    14821 gccccctcct ccggcgccct gccgagcgtc tcgccgatcg agcgggcgtg ctcgcgcacc
    14881 tgcgcctcgt ccgccgcggc atcgcggcgg aaccgcgccg cgtcccggtg ccgtgccgtg
    14941 agcgggaccg aagcgtggct cagggcggcg ccgtgcgtga agcgggaggc gatccccgcc
    15001 gccttgaacc ccgccacccg cacctcgccc gccgccgccc gcgcgaagca gagacgggct
    15061 ccgggggccg cggcgggaag gcgctcctcg cccggagcga caacggccgc cggaagcccc
    15121 agcccgcgca gggcgtcggc cagcgactcg gcgacgagcc gcgcgaaatc gcgggggcgc
    15181 tcggcccagg gccccgggat gctggccgcg accgcgaagg tgacctcttc gtcgtggagc
    15241 acggcgtcgc ctcccgtcgg gcggcgcacc acgtccacgc ccgcggccgc gatcgcctcc
    15301 ggattccacc cctcgggata gggctcggcg cggcccaggc tgagtgtggg gcgcgcccaa
    15361 gcgtaggtcc gggcggcgaa gcgggacggg gccagggcgg cccagcggag catcgattcg
    15421 tccaggcgca tctgttccaa gccggagagg acgagatgcg ggaggaggag cccggacggg
    15481 cgcggcacgg ccgtcctaga agcggacgac gccgagccag ctgtcgagca ggaacatcac
    15541 gcggccgatg aggagcgaga cgcgcgccat cacggtgtac ccgtacgagg cgccgaagct
    15601 caccatcagg aagtagatcc ccactttcgc gatgccgccg atggcgccct tgtgctcggt
    15661 ggagaagaag aagaagacca gcgtcgccaa gagccccagc acgagcagga tgttgttgaa
    15721 cgacgcgtac gcgttccccg ccacccagaa cgggaggatg ctcccgttga tctgctgtac
    15781 caggtcgctc tgggcgaagc cggtgatccg gagcgccggg tagacgccga ggaccatcgc
    15841 gatcggccag cgcgacagcc agtcgccgcg cgggaagaag cgcgagaaga ggaagagcga
    15901 caggaggagc ggcatcacga gcaggagatg gcggtcgaac gtgaagggaa cgccgaagag
    15961 cggatcgacc aggttcggct tgatcgtgcc gtagtaggtg agcacgaccc agtagccggc
    16021 cgagatcccc acgaagaggt gctcgcagaa cttgtagacc gggttgtcct tgtagaggaa
    16081 ggagaagatc cccagcgtca gaagcgccga gatccagacg cccggcagcg tgctcaggtg
    16141 catgcctagc gcctccgttc cttgtcctcg cccaggaacg ccaggttccc gaggaggatc
    16201 atgaacgcga tgaagagatg cgcgagcgac tgcgcgtcca tgccgcgcgt cgcaagcgcg
    16261 ggcttctgga tcaggacctc gtactctgcg ccgcccgcca tccctccgag caggcccttc
    16321 agctgccccg cctggaggta ggggtagtac tcgggcgcgc tcacggcggt gacgcccgcc
    16381 accatcggca ggtggtagcg cgacacgacc tgctgcaccc actccttcgt gccgggatag
    16441 cccgcgctga tgttcacgag cagcgggaag ttcgcatagt tctggacccc gcgcatcacg
    16501 gggatctggc tcaccgggat cccccggcga tccaccggga acgtgcgcgg aatgctggag
    16561 cccatctgcg acatgaccag ctcattccca gccttgtagc cgagattgac gtaatcgacg
    16621 ccttctttct tgccgtactc gcctccggcg atgcgcagca cctggtcggt cagcgggggg
    16681 ccgccgggat agagcgcgag acccacgatc ttgatgttcc ggcgcatcag gtgccgcgcc
    16741 gcggccaggt gcatcgggta gttctcggcg accgtcgagg gatcgtagtc gccggcgagg
    16801 agcaccgtgg agcccgccgg caccttctcg atcgcgtcgt agaagccgcg cgcgcgcgcg
    16861 ctgggctgga cgggggcgat gtggattccc accgccagcg gcacgacgac gagcaggccg
    16921 accgcgagga agatccagcg ccggtccagc ttccggaggg cggtgaggcc gctcatgcgc
    16981 cgcctccgcc gctgctcgat ccgtacgcgc gctccatgcc caggatcagc cggatgccgg
    17041 tcgccatgac gccgatcgcc gctccgatca ggatcccccg cttggccgcg ttctgcggga
    17101 cttccatcag ccagtcggcc gcggccggga tgaagggatg gatcgcgttt ccgatgggca
    17161 cgcggccgat catgaggatg aaggccgcga tcgcgagcag gagggcctcg agcgtgcgga
    17221 tccggaacgc ccggaacgcc gccgacgcaa tgaagaaggc gagcagcgag aacatcgtcg
    17281 ccgacatcgc cgagtagaag gtgttgtaca tccagctgaa gccggaggtc ggatcgaggt
    17341 agtgcgaccc ctcggtgaag ccgacgatca tcatcgcgac cataccgaga agaaggacga
    17401 tcttgtaggg ccagtcgcgc tcccgccccg acacctgctt caggttgatc cggaggagat
    17461 tggccacgcc caggacgtag gtggcgccca cgatcacgat cacccactgc tggatctcgt
    17521 cgtagaacgt gcggatggcg ggatgcggaa cgaagaagcc gatcaggcag aagatgcccg
    17581 cgaggagggt gatcaggaac ggaacctgcc gcttcacgcg cgccccctca ttccgttccg
    17641 aaccagaggc cgagggccgg gttgccgaac gtggccatgg cgcagccgat gatgatcacc
    17701 acgatcagga ccagcttcac gatgtcggcg gacttgaggc tgcccaacag cagcggctcg
    17761 ttcgagagga gcgcgctcgc cgcgtagtac tcctcgccga tcagggtgta gtcgcacgcg
    17821 accacgaaga agggcatctg gtgcacgttc gcggtcccgg cgatctggat cgcgccgatc
    17881 gcatgtcccg tctccgcgag gaggagcgat tcggcgaaga aggccccgag gaggaggttc
    17941 gcggccggtc gctcgcgcag catcatcccg ttcaccgagg cgacgtaggc gaactgctcc
    18001 gaggtgacgt agcgcacctg atccgactgg taggcatcgg cgcgaccgac gtcctgatag
    18061 gcggacttca cgacctcatc ggcgacggtg tagacggccg gagcgttggt gggcaccgtg
    18121 agccgcgtct cgtacttggc cgtcgtccgc gccacgttgc gcaagataat caggctggcg
    18181 atcgtctgga tgtcgtcgat gtcctggacg ccggggatgt agaggacgcc cttccccatc
    18241 tccgtcgcgc ggccgatcgc ctcttcgatg gcgatgagcc cggggatggt ccggaacttc
    18301 cagcggacgc cgcgatgcgc ctggttgatg aagtagaaga ggacgatgaa gaagaggagc
    18361 gtgacgacga ggacgttcac cttggtgctg tcgatccact cgggaccggg cgtggccgcc
    18421 gcggaaatgt ccgagttcag cgccggattc ggcggatcgg cgatgagccg gtattcgtag
    18481 gtcgtgccgt cctggacgcc ctgggcgcct tccccctcgt cggtgtgcgt cttcatcgcg
    18541 ccgtccagcg tgtccaccgg cgcgaacgtg ccgccgcgct cgcggcgcag caggatccag
    18601 tgcgcgggcg cgggggtggg cgaggtccag gtgagggtga cgctctggcc gcggtcgttg
    18661 ggcgtgttga tggcgttcag gggaaacgcg gcggcaaagc cggcgaagac gacgagggca
    18721 agggcggccc ccgcgagcgc cacggcggaa cggccagcgc gaccgaggga gcggatcagc
    18781 ggcatcccct ggcggagacg accgcctccc ggcctgcgtc accacggaaa tccatcacgc
    18841 gcgcgcgttc ctcgatgaac atgaccggcc ggctgaggtc tctaccctaa gtcgaagcgg
    18901 ccccggtgtc aagccgagag ccgcgcggcc gcgccgggcg gaccgggtct aggactcgat
    18961 catctcgacg ttggcgcgcg gcagcatcgt ccgcgagccg ttcccgaagt ccacctccag
    19021 gacccgcacc ttggcctcgg tctcgagcgc cgtgagctcc gccggaagcg ccgtcacctt
    19081 gccgagccgc ccgaagtagg gctgccggat gacgcggatc aggctgccga cctcgagccc
    19141 gccctcgtgc gcgccgtcgc cggagcccgg ctgaggcgcg gccgactcca ggcgcggcgc
    19201 gatgatctcg gggcgcagca cgcccgcgcg gatctgcgtc gcgccgctga ccgagacctt
    19261 gcggccctcg cactgcttca ggagctcgaa cgtccggttc gccatcggaa tctcgccgaa
    19321 gccctccgtc acgaccagcg tgagcccctt ctcttcggag ccggtgatcg cgacccccag
    19381 gtcgtagccc aggaactcgc ggaggtcctg gtcgtcgaaa ccgccgatca cgatcgcctt
    19441 gacccccatc gccacggcgt gccggagcgt cgccgtcgtc acgtgcgcgc ctccgacgac
    19501 cacgcacccg cggtgcgcct cggtgatcgc ggcgctcttc agagtctcgc ccggcgagcc
    19561 ggcgacgacg cgcagcacgc cgctcgtctc cccgccgatg ccgaagatgc cctggatgaa
    19621 cgtgccccgg gtctccacgg tcacgccctg gttgggatgg acctcgacga tcgtcccgtc
    19681 cacgtaggcg tccacctcga cgggaatcgg agcctcgcgc agcagcgcct gccccgtgac
    19741 ggtggagacg ctctcgagcg tcccgtcgat gggagacggg accttcgagc ggaagagccc
    19801 gaagaaggag cggctctcgg cgaacggctc ccccttggcc accgcggagc cgacgggctt
    19861 cacgaggcat tccgtcacgt cctccgggag caccccgagg atgttcgccg cgttgaccgt
    19921 ctgcacgttg ccggggagct cggtgcgggc gacgaccgtc tccgccttga ccgcgtcgcc
    19981 gaccttcacg agcaccgttc ccgccagcgg cagcttgcgc tcgcgccgga ccacggcata
    20041 ggcggtgacc ttgaggccgg gagtatagga atgcgccacg ttgtctcctg atgccgtgcc
    20101 gagtccctcg ggggatcggc tccggaaggt ttaggctccc gcgcccacgg cggcgggtgc
    20161 gcctgcgccc gctagggccg ccttggaggc cggatagatg tcgagcgcct ggttccactc
    20221 ggcgagcttc gcgatgcggc gcgcgcgatc ctcgggcagg ccgaacggac gccgcccacg
    20281 gcagtcgaag atgaggccga ccacgccgcc gcgcagcgtc accgtccgct cccgtccctt
    20341 cccctcgccc acgtcgaacg acttctcggg gcgaagcgtc gccttggcgc gctccccgtc
    20401 ggcgagcggc acgagcttca gatagccgta cgggaccggg aactcctcct tcttcccgcc
    20461 cggcagctcg agggagaccc ggagcatcgt ctgcccctcc ttccccgggc cgaccggcgc
    20521 cacgctggtg cccaggcgga tcaggcagtc cttctcgaag acctgccgtg ccgccaccgg
    20581 gtgcaccgtc gagagcacgc ccagctgcgg cgccatgaag atcgaatcca ccgcgagctc
    20641 cgtcacgtgc tcgggaagga aggcgtcgag catcatcagc gcggcctgcg agcggcgggg
    20701 cgcgtgcgac agcacgccgc cgctgcccac gagcagatcg agatggtcga gacgcaccag
    20761 ggtctcgccg gaggccgtct gctcgaacgt gtcggagatg gtgcgctcct gctgcacgcc
    20821 cttcagtccg acggcgagct gcttgtgctg ctcgaacgcc agccggagcg cctcgcgcga
    20881 gattgcctgc tcgatcacca gctcctcgag cgactgcgga atggtcgtgg ggcggatcat
    20941 cttgttcttg atccgattgc gcagatcgag ctcgtcgatg gcgaacggca cccagcgcat
    21001 gatgttcggg agccccgcct cggccagcac gttcgacacg ctgtagctca tcccgagatt
    21061 ggcgctcacg gtgcggttga agacaccctg gaacacggaa aagacgtcgg tggtcgcgcc
    21121 gccgatgtcg acgccgatca cgttgatctt ctcgtcgcgc gcgatcgtct cgatgatgag
    21181 cccgaccgcg ccgggggtcg gcatgatcgg gatcggcgac caggtcatca gcttcttgta
    21241 cccgggcgcg tgcgccatca cgtgctccat gaagagatcg tggatcttgt cgcgcgccgg
    21301 ggtcaggttc tcccgctcca gcacgggacg caggttgtcg gtgatcacca gcgccgtctt
    21361 gtcctccagc cggtcccgca cgatctgcgc cgcgtccttg ttgccggcgt agatcaccgg
    21421 gagctcgtag cccgcgccga ggcgcgcctt gggctccgcg gccgccagga gctcggccat
    21481 ctcggcgacg tgggaggtgg tgccgccgtc cacgccaccc gagaggagga tcatgtcggg
    21541 gcggagctgg cggatccgct tgatcttctc gtgcggaagc cggccgtcgt tggaggcgat
    21601 gacgtccatc acgatcgcgc ccgcgcccag cgcggcgcgc tgcgcgctct cccccgtcat
    21661 gctcttcacc acgccggaga ccatcatctg gagtccgccg cccgcgctgg acgtggagat
    21721 gtagaggtcc acgccttcat tcccctgctg cggcgtgatg atccgctcgc cgtccaggat
    21781 cttccggccc gccagctcct ccacctccat gatcgcgttc agcacgcccc gggtgacgtc
    21841 ctcgaagggc gcctcgaccg tggtcggcgc ctcgccgcgc acgatcaggc ggtactcgtc
    21901 cccacggcgc tcgatcagga tcgccttggt ggtagtgctg ccgcagtcgg tcgccaggat
    21961 cgaccggagg ctctcggccg gcacgggcag ctgggcggcc gcatgaatct gatgttcggt
    22021 ctgggtcact cggattcctt tcgtttccgg agcgcggcgc gttcgtcgcg ctcgcgctgc
    22081 ttcttttcct tctcgctgcg gcgctcttcg tcgtcccggc gctcgatcgc gaccaggagc
    22141 gcctcgacgc tctcgcggcg ctccatgagg gccgcgaagc gctcgtactc gggcaccgcg
    22201 aacagcgccc gcaccatcgg ctcgaagaag tacatcgcct ggctgcccac gaacgagagc
    22261 ggcttcatgc tctcgaggaa caggatcgcg gggaccgtca tgcggtagcg cacggtggtc
    22321 tcggcgaggc ggtccaggag acgccggtcc tcgtcggtca gcgcctcacc ggcgcccggc
    22381 gcgcgcgacg cgtgcttgaa cgaacgccac caccccatcg cggttccctc ccaacgggag
    22441 ccgcacgccc cggtaggcgt ccagccagct ccgcttctcg aaggggctca ggaagagccc
    22501 cgcctcgtct tcttcgaagc gcttcatctc gctccagggg cgcgcccgca cgacgccgag
    22561 cgagcgcacc tccacggcgt ccggcgtcag ccggtagcgc gtggggagga ggtacgacga
    22621 cagggaaagg agcacgaaga tcggcgcggc gatgccgagc agccggtccc ccgtgccacg
    22681 ggcgatcaat accccgagca cgacggcgcc ggccacgatc accacggccg tcagcggctt
    22741 ctgccgggcg ggccagacgc tccaactgag ctccgcgccg tcggcctccg gggcggtgcc
    22801 ccggtgctcc gagaccgctt ccgcctcctc ggctccggcg ttccccaggc gcgcggccgc
    22861 ggccgggcgc tcacgcgcca cccgcggcct ccgcgtcctg ccaggaaagg acgagctgcc
    22921 gggcggctcg cgcctcgtcc aggcggcgca ccggcgtgcg cgtgggagcg tctttcagct
    22981 tctgcggcgc ctcgatcgac tcccggtcga tcgcgagcat cgcctcggcg aacgcgtcca
    23041 gcgaggcctt ggtctcggtc tcggtcggct cgaccatcag cgcctcgtcc acgatcagcg
    23101 ggaagtacgt cgtgggcgcg tggacgccga aatcgaggag ccgcttcgcc acgtcgagcg
    23161 cgcgcgcccc ccgggccttc tggcggctgg ccgagagcac gaactcgtgc atgcagtagc
    23221 cggcgtggga cggctcgtac gcctcgatca gccgggcctt gaggtagttc gcgttcagga
    23281 tcgcctcgcg gctcacctcc gcgatgcccg aagccccgag cgagcgcatg taggtgaggg
    23341 cgcgaaccag gatgccgaag tttccgtaga acgagtggac ccgtccgatc gagcgcggcc
    23401 ggtcctcgtc ccagtgccag cagtcggcct ggcggacgag caccggcgcg gggagatagg
    23461 gctcgagctc ggcccggacg cagagaacgc cgccgccggg gccgccgccg ccgtgcggcg
    23521 tgctgaaggt cttgtggagg ttgatgtgcg cgaagtcgaa gcccatccgg cccgggctgg
    23581 ccagaccgac catggcgttc aggttcgcgc catcgaggta caggagcccg cccgcgtcgt
    23641 ggacgatgcg cgcgatctcc cccacgttgt gctcgaagat cccgagcgtg ttcggattcg
    23701 tcagcatgag cgcggcggtc cgcggcgata ccgcggcgcg gagcgcttcg aggtccacgc
    23761 agccgtccgg ccccgagggc acggtgacga ccttgtagcc gacctgggcc accgacgagg
    23821 gattcgtgcc gtgcgccgag tcggggacga tcacctcttc gcgcgcgtcg ccgcgctcgg
    23881 cgtggtaggc gcgcgtcatg aggatcgccg tcagctcgca ctgggcgccg gccgccggct
    23941 gcgtcgtgac gccgggcatc tccatgatcg cgcggatcca ctcgtgcagc tcgccgatca
    24001 ccgcgagcgc tccctgcgcc gtctcctcgg gctgcagcgg atggatcgcc gaaaatcccg
    24061 ggatgcgggc catctcgtcg ttgagcttcg ggttgtactt catcgtgcag gagccgagcg
    24121 gatagatggc gcgatcgatg tgatggttca tgaccgagag gttcacgaag tggcgtacca
    24181 ccgccggctc ggagatctgc ggcagatcgg gagccgccgg acggagccgg acgccgggca
    24241 acagccgcgc cgcgccctgc tccgggacgt cgagcggtgc caggcgcgga ccgcggtggc
    24301 ccttgacgga gccttcgtgc agggtcagct cggacatacg gcctcctcgg gcgccggccg
    24361 ccccgcccgc gcccatcgcg cgagcgtctc cacgtagcga tccatctctt ccttgctccg
    24421 cttctcggtc gccgccacga ggagatccgc cgggcgatcg gcgtcgaagc gcgagagcgg
    24481 aacgcccgcg aggatgcgcg agccgagcgc ggcgtgcacg aagcgctcgg ccgagcccgg
    24541 caggcggacg acgaactccc ggaagaaggg cgcgccgccg tgcgcgagcg tcacgcccgg
    24601 gacggc
//
LOCUS       DASSLZ010000255         4369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1092267, whole genome shotgun sequence.
ACCESSION   DASSLZ010000255 DASSLZ010000000
VERSION     DASSLZ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092267"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFM00_09680"
     CDS             complement(<1..200)
                     /locus_tag="VFM00_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498558.1"
                     /translation="MGHAWDAALFRAVHLGWHQAWLDPIMRALSSPGVFKIPLLALLG
                     AAFLLRGRRGRLGILVLALAIA"
     gene            complement(210..3413)
                     /locus_tag="VFM00_09685"
     CDS             complement(210..3413)
                     /locus_tag="VFM00_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498559.1"
                     /translation="MTRHHDRRAGSVARGGALPRAALTLLLLLALAPAARAQTFGQNK
                     VQYKDFHWKVLRTEHFDVYHYQGTERAVQDAAVMAERGYQRLSRVLQHQIKARVPLVL
                     YASHTDFEQTNITPELLGIGTGGVTEFLKRRVFLPFTGSYAELDHVLTHELVHAFQVD
                     ILFGDRPGIVGNPFASVPPGWFMEGMAEYLSVGEIDTNTQSWLRDASLEGYLIPISTL
                     AYVGDIRVYRFGQSIFEFIADTYGIQKIGDILKRTRRMGSMERALEASTGLTVDVLSK
                     KWQESVRKQYLPQIADYDKADAIASKLTDAQHDRSNFNVAVGVSPSGMQMVYISDKSM
                     YNNVYLASALDGKNFKKLVEGERTPSFETLRFFNTSIAWSPDEKMIALPAKVGGEDAI
                     YLIEIPSGKVKRKLKFGLDAIYSPTWSPDGKQIAFVGIDHGWSHLIVSDTDGKNRREL
                     ISGPYSVREPAWSPDGTKIAYATDQGEGTDVSRLIFGKLRLATFDVATRQVTVLPRQS
                     GSNFSPQWLPDGKSLLFVSDRAGIPNIYRFDLVTGDSFRLSNLLTGVSGVVADAPCIS
                     LSADGKRLLFTSFTRGGWDIYSVRDVRKFLAPATGVVAEQSMPLAPLGTRVEVAAGVT
                     EASGTDPDGDSDPQAAPPVRTAPADSASARAAALRAASQAGSASTLSGSVLRDSVTTG
                     GVPAAVALRDTSTVADTTLAMYVQQVYREPLTDSTTFLRLPYKARFSRDYISGGALFA
                     SNVGFAGSSVISFSDVLGNHNVLAVLNLYGDVTQSDIYLAYTNLAKRTNYGFAVFQYR
                     NDLLLLSAPSSDDVESQIYRGGAVFFSRPFNRFRRIEYGVEAAVLNERVLRFNYDLGQ
                     VSTVANNGDFFYVAPNIGLVADNALYGTTGPINGGRSRYTVETALGDVHYTTGLMDWR
                     RYTNIHQNFSIAQRFIGGASFGRDPQLFRFGGAFTYRGVDYGDLIGTRVLLGNLEFRF
                     PLIEQLRLGWPLAINLGGINGVLFTDAATTWEDGHQPVFFSNVNGLRTRDLHLAFGFG
                     ARVNLGYFILRYDYAREHKLDGGIGKPRHFVTFGAEF"
     gene            complement(3418..>4369)
                     /locus_tag="VFM00_09690"
     CDS             complement(3418..>4369)
                     /locus_tag="VFM00_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET7498560.1"
                     /translation="GRLDWARRFDHMQQHTGQHILSRAFVEVARADTRSFHLGEAVVT
                     IDVDHPGPDAGLLRKVEDRANAVVWEDRPVATHVVSLDRAREFPLRKAPDVEGEVRVV
                     EVEGFDWSACGGTHVARSGQVGMIALLGTEKYKGGTRVAFACGGRALARLREASDVLR
                     GLCLEFTSGEADLPRAIARLKGEVRRLESRLKPLLAESLVREAETLLAAAEPGGSGPV
                     VARYFPDRDPAELAAIAAHVAGRGAIALLAAGRDIARAHFSAPKGTISVGALFAGLAS
                     AYGAKGGGRPEAAQGAIPADRAEEAIDAARRAALAGTNQG"
BASE COUNT          702 a         1440 c         1556 g          671 t
ORIGIN      
        1 caggcgatcg cgagcgccag gaccaggatc cccagccttc cgcggcgtcc gcgcagcagg
       61 aacgccgcgc cgaggagggc gagcagcggg atcttgaaga cgcccgggct ggagagggcc
      121 cgcatgatcg ggtcgagcca ggcctgatgc cacccgagat ggaccgcgcg gaagagcgcg
      181 gcgtcccatg cgtgccccac ggcgcggggc tagaactcgg cgccgaacgt cacgaagtgc
      241 cggggcttgc cgatcccgcc gtccagcttg tgctcccgcg cgtagtcgta gcggaggatg
      301 aagtagccca ggttgacgcg cgcgccgaac ccgaacgcca ggtgcaggtc cctggtgcgg
      361 agcccgttca cgttcgagaa gaagaccggc tggtgcccgt cctcccaggt ggtcgcggcg
      421 tcggtgaaga ggacgccgtt gatgccgccc aggttgatcg cgagcggcca tccgaggcgg
      481 agctgctcga tcagagggaa gcggaattcg agattcccca ggagcacgcg cgtgccgatg
      541 aggtcgccgt agtccacgcc gcggtaggtg aacgccccgc cgaagcggaa gagctgcggg
      601 tcgcgcccga acgaggcgcc gccgatgaag cgctgggcga tcgagaagtt ctggtggatg
      661 ttggtgtagc gccgccagtc catgaggccg gtcgtgtagt gcacgtcgcc gagcgccgtt
      721 tccacggtgt agcgggagcg cccgccgttg atcggcccgg tggtgccata gagcgcgttg
      781 tccgccacca gcccgatgtt cggcgcgacg tagaagaaat ccccgttgtt cgccaccgtg
      841 gagacctgcc ccaggtcgta gttgaagcgg agcacccgct cgttcagcac cgccgcctcg
      901 accccgtact cgatgcgacg gaaccggttg aacggccgcg agaagaagac cgcgcccccg
      961 cggtagatct ggctctccac gtcgtcactg gacggcgccg agaggagcag caggtcgttc
     1021 cggtactgga agaccgcgaa gccgtagttc gtgcgcttcg cgagattggt gtaggccagg
     1081 tagatgtccg actgcgtcac gtcgccgtac aggttcagca cggccaggac gttgtgattc
     1141 ccgagcacgt cgctgaagga gatgacgctc gaccccgcga agccgacgtt cgaggcgaag
     1201 agcgccccgc ccgaaatgta gtcgcgcgag aaccgggcct tgtacggcag gcgcaggaag
     1261 gtcgtgctgt cggtcagggg ctcgcggtag acctgctgca cgtacatggc gagggtcgtg
     1321 tcggcgacgg tcgaggtgtc ccgcagcgcc acggccgcgg ggacgccgcc cgtcgtcact
     1381 gaatcccgaa gaaccgaacc gctaagagtg gaagccgacc ctgcctgtga cgccgcgcgc
     1441 aaggcggcgg cgcgtgcgga ggccgagtcg gccggcgcgg tgcgcaccgg aggcgctgcc
     1501 tgaggatcgg aatcgccgtc gggatcggtg cccgacgctt cggtcacccc cgccgcgact
     1561 tcgacccgcg tgccgagcgg tgccagcggc atcgactgct cggccacgac gccggtcgcc
     1621 ggcgccagga acttgcgcac gtcgcgcacc gaatagatgt cccatccgcc gcgcgtgaag
     1681 gaggtgaaga ggagacgctt gccgtcggcc gagagcgaga tgcagggggc gtccgcgacg
     1741 acgcccgaga ccccggtcag caggttcgag agccggaacg aatcgcccgt gacgagatcg
     1801 aaccgataga tgttcggaat tcccgcgcgg tcggacacga agaggagcga cttgccgtcg
     1861 ggcagccact gcggcgagaa attggacccg ctctgccgcg ggagcacggt gacctggcgc
     1921 gtcgccacgt cgaaggtggc caggcggagc ttgccgaaga tgagccgcga gacgtcggtc
     1981 ccctccccct ggtcggtggc gtaggcgatc ttcgtcccgt cgggagacca cgccggctcg
     2041 cgaaccgagt acggtccgct gatcagctcc cggcggttct tgccgtcggt gtccgaaacg
     2101 atcaggtgcg accagccgtg gtcgatcccc acgaaggcga tctgcttgcc gtccggcgac
     2161 caggtcggcg agtagatggc gtccaggccg aacttcagct tccgctttac ctttccggaa
     2221 gggatctcga tcaggtagat cgcgtcctcg ccccccactt tggcgggaag cgcgatcatc
     2281 ttttcgtcgg gcgaccaggc gatcgacgtg ttgaagaagc ggagcgtctc gaaggagggc
     2341 gtgcgttccc cctcgaccag cttcttgaag ttcttcccgt cgagcgccga ggcgagatag
     2401 acgttgttgt acatgctctt gtcggagatg tagaccatct gcatcccgga cggcgagacg
     2461 cccaccgcga cgttgaagtt ggagcggtcg tgctgcgcgt cggtcagctt cgaggcgatc
     2521 gcgtccgcct tgtcgtagtc ggcgatctgc ggcaggtact gcttgcggac cgactcctgc
     2581 cacttcttgg agagaacgtc gacggtgagg ccggtagagg cttccagcgc ccgctccatg
     2641 ctccccatgc gccgcgtgcg tttcaggatg tcgccgatct tctggatccc gtaggtgtcg
     2701 gcgatgaact cgaagatcga ctgtccgaag cggtagacgc ggatgtcgcc gacgtaggcc
     2761 agcgtgctga tcgggatgag atagccctcc agcgaggcgt cccgcagcca ggactgcgta
     2821 ttcgtgtcga tctcgcccac cgagaggtac tcggccatcc cctccatgaa ccagccggga
     2881 ggcacgcttg cgaaggggtt ccccacgatg cccggccggt cgccgaagag gatgtccacc
     2941 tggaacgcgt gcaccagctc gtgcgtgagg acgtggtcca gctcggcgta ggagccggtg
     3001 aagggaagaa agacgcgccg cttcaggaac tcggtcacgc cgccggtgcc gatgccgagc
     3061 agctccggcg tgatgttggt ctgctcgaag tcggtgtgcg aggcgtagag gacgagcgga
     3121 acgcgcgcct tgatctggtg ctggaggacc cggctgagcc gctggtagcc gcgctcggcc
     3181 atgacggcgg cgtcctgcac cgcgcgctcg gtcccctggt agtggtagac gtcgaagtgc
     3241 tcggtccgga gcaccttcca atggaagtct ttgtactgga ccttgttctg gccgaaggtc
     3301 tgcgcccgcg ccgcgggggc cagggcgagg agaaggagca gcgtcagcgc ggcgcgcgga
     3361 agcgctccgc cgcgagcgac ggatccggcg cgccgatcgt gatgtcgggt catccattca
     3421 cccctggttc gttccggcca atgcggcccg gcgcgccgcg tcgatcgcct cttccgcgcg
     3481 gtcggcggga atcgctccct gtgccgcttc ggggcgcccg ccccccttcg cgccataggc
     3541 gctggcgagg cctgcgaaga gggcgcctac ggatattgta cctttcggag ccgaaaagtg
     3601 cgcccgcgcg atgtcccgcc ccgccgcgag gagcgcgatt gcgccccgcc cggcgacgtg
     3661 cgccgcgatg gcggcgagct cggcgggatc ccggtcgggg aaataccggg cgaccaccgg
     3721 gcccgagccg cccggctccg ccgccgccag cagggtctcg gcctcgcgga ccagggactc
     3781 ggcgaggagc ggcttcaggc gggattcgag gcgccggacc tcgcctttga gacgcgcgat
     3841 ggcgcgaggg agatccgctt ctcccgacgt gaattcgaga cagaggccgc gcaggacgtc
     3901 cgaggcctcc cgcagccgcg ccagcgcgcg gccgccgcag gcgaaggcga cgcgcgtccc
     3961 gcccttgtac ttctccgtgc cgagaagggc gatcatcccg acctgccccg aacgcgcgac
     4021 gtgggtcccc ccgcacgccg accagtcgaa tccctccacc tcgacgacgc ggacctctcc
     4081 ttccacgtcc ggcgccttgc gcagcgggaa ttcccgcgcg cgatcgagcg agacgacgtg
     4141 ggtcgcgacc ggccggtcct cccacacgac ggcgttggcg cgatcctcga ccttgcggag
     4201 gagtccggcg tccggtcccg gatggtccac gtcgatggtc acgaccgctt cgcccaggtg
     4261 gaaggagcgc gtgtcggccc gggccacctc gacgaaggcg cgggagagga tgtgctgtcc
     4321 cgtgtgctgc tgcatgtggt cgaagcggcg cgcccagtcc aggcgtcct
//
LOCUS       DASSLZ010000256        22721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1350241, whole genome shotgun sequence.
ACCESSION   DASSLZ010000256 DASSLZ010000000
VERSION     DASSLZ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 22721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22721
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350241"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="VFM00_09695"
     CDS             <1..1521
                     /locus_tag="VFM00_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7498561.1"
                     /translation="YRVLVESSPQGLLIVQGGKAVFANRAIAQMADRLESELLHPGFD
                     PLIIFDGEAQESLREALEGANAAAVQRTTEIVQPVGRRIPVEVQTAPLVFQNHPAAMV
                     LVSDVSALRDLQDRLVRGEKLRALGELAAGVAHDFNNNLGIILGRTQLLLSRTGDPAL
                     VSGLNVIRQAAMDGGEAVRRIQQFSRVREDRAHEPLDLTAIAEEVVEITRGKWKNDAE
                     RRGVKVEVRIEAADAPSILGSRAEIREAMTNLIFNAVDALPRGGRIDVRCRAEAGQAV
                     IEVADDGVGMDEDVRSRMFEPFFTTKGLSGTGLGLSMVYGIVSRHQGTIEVETGVGQG
                     TVVRMRFPVTDQLERPASPARPEPSRVAARVLVVDDELELLHVLEEALSAAGHVVTAA
                     SSGLEGIERFREGAFDVVLTDLGMADVSGWELARTVRQEGSASVVLGLVTGWGATISQ
                     EMVVGHGVDFVVAKPFDVEDLIARTNRAIESKQGLAAAADPAIPPTDASPSGRRRT"
     gene            complement(1475..1984)
                     /locus_tag="VFM00_09700"
     CDS             complement(1475..1984)
                     /locus_tag="VFM00_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7498562.1"
                     /translation="MSGSTTTRIDGPLVTLEVPGPEHLPAIWKVYLDAPDYFEALTGS
                     SDFDPTDVEMMYEEAIADESRFYFAVRRTDTKSLIGTIEIESGSDEHPAALRGLVIAQ
                     GERGKGYGRAALEMAEQFLGDELGVPSIAAEVPEGYDDGERFLEAMGYRQRRLRSGDV
                     RWVKRLSEE"
     gene            complement(1981..4941)
                     /locus_tag="VFM00_09705"
     CDS             complement(1981..4941)
                     /locus_tag="VFM00_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HET7498563.1"
                     /translation="MRAVFGIALACLLMPAGRVSAAPPLYPSAPGRRATAFQAPRIPL
                     RQPGLNAPSQVRTTVGSKYKVLVILLQFSDHPADTLNHTPADFADLLFSKGTRPGGSF
                     RDYYNEVSGGAFDVDGVVTRWYTAPRPYTDYVGTDGGQGPGPLNAQVMALDAVRLAEP
                     DVDFTQFDNDGPDGIPHDGDDDGEVDGIFIVHAGPGAEETGDPTSDIWSHKWNVPGPR
                     GGTSADGVIVYDYTTEPEEWFLTTPWTTKGALMTIGVFCHEFGHVLGLPDLYDTSGLP
                     GANEGLGEWDLMASGLFNHLPDQTVGSCPAHLSAWSKEALGWLQPTWVTRDSAGVTIP
                     PVETSGRAFRLWTNGVEAGEYFLVENRQPIGFDAALVKSTMERDSTAAHGLMIYHVDT
                     GIIGNNTASHKQVDVEEGGGIEAASGFTGVQNLDLARGSAASEPACEGSVIVVGNRGD
                     AYDPWPGAGARAAFDANSCPNSDSYCGGISQVAIRNIAETGAEPRDVTADFFVSGTSV
                     RRNAVTVDDSPFLGNNNNGNGLVEPGETVRIHIPLQNLDTTPTGVLTARVAVTEPYAG
                     LLADSVYYGVLGGAASDTGSVIFAVINPTPDPRGCNLTIAVSDAAGLVLADSVQILIG
                     QRSGICEDFENATARRWVSVPLGCGGVSEWHREAGVNHTPGGSWAWRLGPFGFIGHYA
                     PSQDARLVSQPVRLTGTSDTLSFWQRYDSEFAFDGLTVEISTNSGATWTPLEPVGGYN
                     TGDRFSGTQTTFTRVDVPLTGYSGIVQFAFRFRSEPPNEGLGWWIDDVLVGGTSACAT
                     VAIAIASFDAAAGPAGGPAAVRLSWSVADGAASSVGIDREGDDGTRRRIATVPASDGG
                     YLDTDVAPGAYRYWITASREGEPAAEAGPISVTVGEAPRVFALSSVRPNPFRAGASVA
                     VSLDRSGPFMVRVFTADGRLVRTLARGAGRPSDLRLTWDGTDGRGRPAGAGIYFFELR
                     SGNRTRVQKAVLLR"
     gene            complement(4982..6433)
                     /locus_tag="VFM00_09710"
     CDS             complement(4982..6433)
                     /locus_tag="VFM00_09710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7498564.1"
                     /translation="MNAIDAPTWLFPALNVVSIIVLVYFTALQLVLFTLLIVSFFDMR
                     RRLAQNFYADYEAVAKSHFTFPISVLVPAYNEETTVVEAVRSVLSLDYPQHEVIVIND
                     GSTDGTLERLTREFGLVRREAIVRRSIPTDAILRGIYRSPTIPNLVVVDQAHAGKSAA
                     LNAGINVSRYPLFCTIDADSVFQENALLRVVRPFLEDPDRVIAVGGQVRVANGCTVER
                     GRVVDPHLPQNVLAAFQVVEYLRAFVASRLGLSAMNNLLILSGVFSLFRKDVVVEVGG
                     YRSDVVTEDMELVLRLHHAMREQGRPYSVVFLPDPICWTEVPQGLRTLARQRGRWHRG
                     LIHSLWLHRSIFWEKKSGSLRWLGMPYYFLFELLGPIVEVIGYIVLPIAYFMGFLSPL
                     YFWAFLFLSVTSGAFLSISAVFLEELSFGLYRSWGDFALMIAIGVIENFTYRILTLFF
                     RLGAMVDILLGRGGWGKQERAGFGRAEVASVKP"
     gene            complement(6435..7508)
                     /locus_tag="VFM00_09715"
     CDS             complement(6435..7508)
                     /locus_tag="VFM00_09715"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET7498565.1"
                     /translation="MIDYQALVITLGLLLGILALTTMAAVVNKIHSNREDDSRRRFLD
                     RLRQSFLLLAGGEQRDAAYREIANGLTGRWSQLAAEEVSQLELSLRLDVIRSLEQQGV
                     IAHYLRETKSPLKWSRAHALRLLGELKVPASVPALLAALEDRDPDVRNVAARALGKMK
                     LQAAEEALVGLLGKHEQSVSARIAAICIEMGARTAPLLIRALRDGTPKARFWAARILG
                     EIKDGRAARSLGDALLDPEPDVRSSAAWALGAIADRSTAAMVEAALRDPVWFVRAHAA
                     EALGKIGDPTMAGALGRMLEDRSWWVRKNALDALVLLGEPAKPALLSALQSNDRFARD
                     CATEGLATLGVPAVPPQRAATGG"
     gene            complement(7528..9210)
                     /locus_tag="VFM00_09720"
     CDS             complement(7528..9210)
                     /locus_tag="VFM00_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498566.1"
                     /translation="MSRKPTGTRTRSARPRLAAAWAAFLAASALASLSVSPALADPDP
                     NALSPAKTLERVAAEDSSRSESALAEARALADAKKFDEAARILRARLAQAPEDADAQS
                     LLARVLAWGRHYDESIAEYRKILAAHPDDAFDRAGYARVLAWSGRSEASLREFRRAVR
                     ADSTDLESRVGYARALSWAGDLPGASTEYRGILSSNPAYGDAWLGYATVARWRGAPSS
                     SDRFLERAAHGSADTEAMGQERRAVRDALGPSIGAGLTTAHERQYVAAAPDFAIESTG
                     PFAQARATLARAATVTVRVSRLAQFERRLGPPVLGTTLNYDLDMTVLRADAALTRGYP
                     FLLSAGVESRAIQAGSPNVIYPLRGDDRFFGWNARAWGFAGRWTPSASVRRDYVPIKD
                     DTGLPEIRAGRQTVVAGELAYQWNARATLSAGAEHGSYSDENDRSTLRAGAAYRLRLA
                     RPGLSADYGISFTDFDSSSASYFTPLKSTRHAAGLALTGYSERASLDWGARYQFSAVL
                     SSNFDNIYTSTWSGWVNLVAGGSFPLGLEGSYSRDNNAYETWFVGASGAVRW"
     gene            complement(9170..11596)
                     /locus_tag="VFM00_09725"
     CDS             complement(9170..11596)
                     /locus_tag="VFM00_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498567.1"
                     /translation="MPLLIDAFVWLWVTAASWAAPPAISAHASAHAVPGRTAPAISAA
                     RGPAAAYVRAAGDRFEIVRNGSARPVFFRGVNLGAGMPGHFPGEFAFTKDDYRRYLRF
                     ARELHANAIRVYAVHPPAFYQALREENAAHPDRPIWLFQEVWTELPEGDDFWEASFTG
                     AFDAAIRSAIDAVHGNALVAPRPGHASGRYDADVSPWIAGWLLGREWEPYAVRATERK
                     HPGMTSFRGAFFAVEAGTPMETWLARACETSASYEASRYGTAHAVAFVNWPTLDVMRH
                     PTETERGGREAEHDEDAYSVDPLRIRPLKNAAVDPSFRGYFANYHVYPYYPDFMNTDP
                     GYAAVRDRHGACSYAGYLRDLKAHTRGIPLMIGEYGVPTSRGIAHQQPQGIDHGGASE
                     REQGERDVRLLEDIADAGCSGSLLFALFDEWFKVNWLVARMEAPRDRDPLWHNLLDPE
                     ENYGLIAFDPPPRIRVDGATSDWRGIAPYASDPKGRWLKAFYATSDANRLYLRFDVDP
                     TKETPAALGASIDVLDPARGDTRLPAPLRAVWSRGAEFQLVIEPASALQRNLRDRPGA
                     ELFIDRSMNWSGWSRLLEDGVFVRNRAPFRPVANADGAYVPLLIETNRERVARNGTVY
                     PARHLDWGSLGYGAERSPAGGARDDSSVAYDPHSEWCADPVRGVIEVAIPWGLLNVSD
                     PSSRSVLDDIPETSAVEATPTAGIGFLAWATSARGFSADSYGPSGPKTRPAGPGDSQF
                     LGPPGTTQAETGQEVRVATPAHVSLLWNGWEQPITRERAKESAALVRQAFEGMESRES
                     EANRNQDALR"
     gene            11744..12181
                     /locus_tag="VFM00_09730"
     CDS             11744..12181
                     /locus_tag="VFM00_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7498568.1"
                     /translation="MTLKRMDNIGIVVESLDAVISFFSELGLKLEGRAMIEGEWAGRV
                     TGLGSQRVEIAMLVTPDGHGRLELSRFLSPSVVADHRNAPVNALGYLRVMFTVDDIDE
                     TLARLRKRGAQLVGEVVQYESAYRLCYIRGPEGLLIGLAEELR"
     gene            12272..12711
                     /locus_tag="VFM00_09735"
                     /pseudo
     CDS             12272..12711
                     /locus_tag="VFM00_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
     gene            complement(12728..13468)
                     /locus_tag="VFM00_09740"
     CDS             complement(12728..13468)
                     /locus_tag="VFM00_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET7498569.1"
                     /translation="MSAIETDKRTRFQRRSRSMRLVLFDIDGTLLREGLAPKIAFARA
                     LRETYATTGPINDFKFAGMTDPQCVTEIMRLAGVHDDVIHSRRKECLRRYVEHLHVEM
                     PNHDGARLFPGVRELLERLNKLDDVMVGLLTGNILRGAELKLRRWNLEPYFRFGAYGD
                     DHEDRSVLAQIALEKARALTGRVFSGADTTVVGDTPRDVSCARAIGARAVVVATGQVS
                     RSELAESAPDAILDSFENHEAALRAIFP"
     gene            complement(13539..14642)
                     /locus_tag="VFM00_09745"
     CDS             complement(13539..14642)
                     /locus_tag="VFM00_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET7498570.1"
                     /translation="MPLDVKTLLQATVESGASDLHISSGMPPMIRLRGDMVRSQSPAL
                     SDPDARALLYTLMNDGQRKLFEGQREIDFAFEIEGLSRFRANIFEQRRGIGGVFRVIA
                     TKIRTLEELGTPKILRELALRERGLVLVTGPTGSGKSTTLAAMIDFVNASRAGHIITI
                     EDPIEFVHESKSCLINQREIGAHTDSFAAALRSALREDPDILLVGELRDLETTALAIT
                     AAETGHLVFGTLHTNSAAKTIDRLIDIFPADRQSQVRTMVSESLEGVIAQTLIPTADG
                     KGRVAALEILVGVPALRNLVREDKTTQIQSVMQVGAQHGMQTLDQALKDLVIAGKITR
                     EAAAERSTNPKLFEGPHAGAPGAPAGAAAGARA"
     gene            complement(14642..16075)
                     /locus_tag="VFM00_09750"
     CDS             complement(14642..16075)
                     /locus_tag="VFM00_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET7498571.1"
                     /translation="MAPFPHSTRTLTSILVECGIVTDEQVAQALARQRDTGLLIGETL
                     VELGFTTEENIGWALSRQLGFPYADVHPSSVDPEMVKRFPEGLLRRIQAVPLFGTEEE
                     ITFAMADPTDVDAVAELKAAAGIPASLVIGGPASIRRVLDSVYGPPESGSPHAAPPPT
                     SRRDIVWDRAGTNFLAYHLHAAVQARASEIHFIPAGSDLTVAYRTDAGLVTQTPEQPE
                     TSLYLRARLGVLGIPDVDDGRASSWGAAIVELGPDRVAVSACHSRTDAGVATVLRLAP
                     APVEAPDLSLLGLSPIGEAEIREFIDGPEGLIVVTGPPRAGGSLVLASLAALAARADR
                     RTLVFEPAHLLPYPPGTIRIPATDAARWEQLAVGLGADVVALDGVVDGEQVETLLHGA
                     AVGRLVFVRTDWLDPKALIAHLTRSRAGRAALRDRPFALIALPAARREGSAVWVDPSR
                     AEIQAGSLQVTILSDEKRDALAKEGGR"
     gene            16394..18043
                     /locus_tag="VFM00_09755"
     CDS             16394..18043
                     /locus_tag="VFM00_09755"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HET7498572.1"
                     /translation="MAQALKKDSKKAASTPAVRKPAPLKVPKGVKKIVHLTAADLAEK
                     QREISVSEFFTKNRHLLGFDSPAKALLTTVKEAVDNALDACEEAGILPDILIEIQEIS
                     ETRYRIAVQDNGPGIVKAQVPRIFGQLLYGSKFHRLRQSRGQQGIGISAAGMYGQLTT
                     GKPITVISRPGQGKPAHKFDIQIDTRANRPVVVADHEVEWMPAHGTRVELEIEATYKK
                     GRHSVDGYVEQTAMANPHARMTYVAPNGERLELERVAEEMPKAPLEIKPHPHGVELGI
                     LLKMLHETKAKNLGAFLSGEFSRVGPTVADEIARAAGLPVSYSPHKAARDQAEALYNA
                     IQATKLMAPPSNVLSPIGEELLLEGLKKQIDARFYAATSRSPSVYRGNPFLVEAALAY
                     GAESLPADELITMLRFANRVPLLYQQSACAVTKAVVGTNWKAYGLQQSKGALPAAPMI
                     LMVHIASVWVPFTSESKESVASYPEIAKEIRLALQDVGRKLGIYIRRGLREADAEKKR
                     SYIEKYIPHIGIALQEILAISDKERDKVCDTLKDTLERSRQ"
     gene            18040..19125
                     /locus_tag="VFM00_09760"
     CDS             18040..19125
                     /locus_tag="VFM00_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013798991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET7498573.1"
                     /translation="MSDKVVPKIKKEAKAVEESILKKKKPVLKFPVRSLANVRYLEKE
                     GYFEIRGKKKERTLTVNTVKSFAQTLRMMALSKSLVETDDIATKREAYYVSKNWGDAR
                     FLEQTESDTVMDDVEALFEVNREQMGFVPEEKGGDVSGALTVVDKDPDTGKSLRIDCT
                     KFGSGAYSIPISVEDLEFETKAQFILVIETAGMFQRLVKHGYAKKANCILVSMGGVPT
                     RACRRFIRRLADAKKIPVYVFVDGDPYGITNIYRTFKVGSGNAAHINEFFCVPQARFL
                     GITPQDILDYKLPTHPLKEVDVKRAKDAIKNDPFFHHHKAWATAMEKLISMGVRAEQQ
                     ALAAHGLNYVMDTYLPEKIAHPERFLP"
     gene            19129..20256
                     /gene="pheA"
                     /locus_tag="VFM00_09765"
     CDS             19129..20256
                     /gene="pheA"
                     /locus_tag="VFM00_09765"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET7498574.1"
                     /translation="MGSDLQAVREAIEAVDREILAALKRRMDLVEQVARAKIESATPF
                     RDRQREEQIIGRIRQAAAELGLDAHEIERLYRDIMEMSVAHQQALVRSLEGVPLRVAY
                     QGVEGSFSHLTAQRRYAGREGGVLLTGHDTFRGAVGAVRDGSADFALMPIENTTAGSV
                     HETYDLLAEGGVTITAEVVSRVEHCLLALPGAKLESLRTVISHPQALAQCEAFLRTIP
                     WAATRTEFDTAGSARKVREGNDPTVAAIASESAARLYGLEVLRQGIQTQEGNYTRFVE
                     IAREAVACPPGAACKTSLQIALAHKPGALGEVLARFGARGVNLTRIESRPVLGSPWQY
                     RFYLDLEGHAASEPVRAALEELKAVTTEVRVLGTYPRASEP"
     gene            complement(20270..21040)
                     /locus_tag="VFM00_09770"
     CDS             complement(20270..21040)
                     /locus_tag="VFM00_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498575.1"
                     /translation="MIRLSESHLGTFRDLAKLGSSVDLSLIGALALACALPRFRRQTN
                     DVDITIALPLDEANAALARLPGWTQTGRTEFEWRSASGTLVHAIPLDASSRKQGFLVW
                     PASGRRMTLVGMRHAFEHTNPLNVARGVTIRSASVPAVILMKSVAYQDRPRERERDVD
                     DFATVMDVYLDDAGTRRWDEAQGVTREDAGSYLLGKDVGRIADEVEMKSIEGFVRLLL
                     GGAGLATLVRVSPPLLRANPRDVLSRLEAFRQGLLAGA"
     gene            complement(21037..22092)
                     /locus_tag="VFM00_09775"
     CDS             complement(21037..22092)
                     /locus_tag="VFM00_09775"
                     /inference="COORDINATES: protein motif:HMM:NF021469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HET7498576.1"
                     /translation="MKQHPDLGRFLDRLAELPILTKPREVRRPAMTQPDTCIEITVGG
                     RKRILAVELRRSDPLSRIALDHWMDQRKGSEQDWILLANHVGPKLSRYLREHRINFVD
                     ASGNCHLSIDEGHVAYIEGRKRSTPAWESQGTGRAGTRIQFALLARPELVTRPVREIA
                     SASGASKTAASSVLNRLEREGVIGPTGRDRILLRPDALLQRWLTGYVDRLRPRLVLGR
                     YEARFEDPDELDRFLGAQLSTHGEDRFVEGSKTIRWAWGGAAAEHRLLHYYRGTATVI
                     HFDAPPSTAAKRLGLAPAREGSITFLGVPGPLAFQGTQPNVVHPLLIYSELLATGEER
                     ARDAASRVRERYLQHLS"
     gene            22262..>22721
                     /locus_tag="VFM00_09780"
     CDS             22262..>22721
                     /locus_tag="VFM00_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498577.1"
                     /translation="MRPVPGIAAALLAATLLSTPIAASTSFAAGKIPITKLADLPPHS
                     YKIPDKPSALVTNKAAILELAGAVEKDMKADLDKYDIQDPTAVRRMYGTLLTIAMLKE
                     DYPSAKQLVAQIRDLEDKPAQKLTSGLLAEAIMKAHESGAADLHAAIRSNL"
BASE COUNT         3636 a         7621 c         8092 g         3372 t
ORIGIN      
        1 taccgcgtgc tcgtggagtc cagtccgcag gggctcctca tcgtgcaggg gggaaaggcg
       61 gtcttcgcga accgggccat cgcccagatg gcggaccggc tcgagtcgga gctgctccat
      121 ccggggttcg atccgctcat catcttcgac ggcgaggcgc aggaatcgct gcgggaggct
      181 ctcgagggcg cgaacgccgc ggccgtccag cgcacgaccg agatcgtcca gccggtcggc
      241 cggcggattc ctgtcgaggt gcagacggcg ccgctcgtct ttcagaatca cccggcggcg
      301 atggttctgg tctccgacgt ctccgcgctc cgcgatcttc aagatcgcct ggtgcgcggc
      361 gagaagctgc gcgccctggg cgagctggcg gccggcgtgg cgcacgactt caacaacaac
      421 ctcggcatca tcctgggccg cacgcagctc ctcctgtcgc gcaccggcga cccggcgctc
      481 gtctccgggc tcaacgtgat ccggcaagcc gccatggacg gcggcgaggc ggtgcgccgc
      541 atccagcagt tcagccgcgt gcgcgaggac cgcgcccacg agccgctcga cctgaccgcg
      601 atcgccgagg aggtggtcga gatcacgcgc ggcaagtgga agaacgacgc ggagcggcgc
      661 ggggtcaagg tggaggtccg gatcgaggcg gccgacgcgc cctcgatcct gggttcgcgc
      721 gccgagatcc gggaagcgat gacgaacctg atcttcaacg ccgtcgacgc ccttccgcgc
      781 ggcgggcgga tcgacgtgcg ctgccgcgcc gaggcggggc aggccgtgat cgaggtggcg
      841 gacgacggcg tcggcatgga cgaggacgtc cgaagccgca tgttcgagcc cttcttcacg
      901 acgaagggtc tctcgggaac gggcctgggt ctctcgatgg tctacggcat cgtgtcgcgc
      961 caccagggga cgatcgaggt ggagaccggg gtgggacagg ggacggtggt gcgcatgcgc
     1021 ttccccgtga ccgatcagct ggagcggccg gcctcgccgg cgcgccccga gcccagccgg
     1081 gtggccgcgc gcgtgctggt cgtggacgac gagctggagc tgctccacgt tctcgaggag
     1141 gcgctttcgg ccgcgggaca cgtcgtgacc gccgcctcct ccgggctcga agggatcgag
     1201 cgcttccggg agggagcctt cgacgtggtg ctgaccgacc tgggtatggc ggacgtgagc
     1261 gggtgggagc tggcgcggac ggtgcgccag gaggggagcg cgtcggtcgt tctggggctg
     1321 gtgaccggat ggggggccac gatcagccag gagatggtgg tggggcacgg cgtcgacttc
     1381 gtggtggcga agccgttcga cgtggaggac ctgatcgcac gcaccaaccg cgcgatcgag
     1441 tcgaagcaag gcttggccgc ggccgcggac cccgctattc ctccgacaga cgcttcaccc
     1501 agcggacgtc gccggacctg agccgccgct ggcggtagcc catcgcctcg aggaaacgct
     1561 cgccgtcgtc gtatccctcc ggcacctccg ccgcgatcga aggcacgccc agctcgtcgc
     1621 ccaggaactg ctcggccatc tccagcgcgg cgcggccgta gcccttgccc cgctcgccct
     1681 gggcgatcac gaggccccgg agggcggccg gatgctcgtc ggagcccgac tcgatctcga
     1741 tcgtgccgat caggctcttc gtgtcggtcc gccggaccgc gaagtagaag cggctctcgt
     1801 cggcgatcgc ctcctcgtac atcatctcca cgtcggtcgg gtcgaagtcg gaagagccgg
     1861 tcagcgcctc gaagtaatcg ggagcgtcca ggtagacctt ccagatggcc gggagatgct
     1921 cgggccccgg cacctcgagg gtgaccaggg ggccgtcgat ccgggtggtg gtggagccgc
     1981 tcatcgcagg agcaccgcct tttgcacgcg cgtccggttt ccggagcgca gctcgaagaa
     2041 gtagatcccc gcgcccgccg ggcgtccccg cccgtccgtt ccgtcccagg tgagccgaag
     2101 atcggagggc cggcccgctc cgcgggccag cgtgcggacg agccgtccgt ccgcggtgaa
     2161 cacgcgtacc ataaaggggc cgctccggtc cagggagacg gccaccgagg cccccgcgcg
     2221 gaaggggttc ggccgcaccg acgagagggc gaacacccgg ggcgcttcgc cgaccgtcac
     2281 cgagatcggg cccgcctcgg cggccggctc tccctcgcgg gaggccgtga tccagtagcg
     2341 gtaggcgccc ggcgccacgt ccgtgtccag gtagccgccg tcggaggcgg gcaccgtggc
     2401 gatccgccgt cgcgtgccgt cgtcgccctc gcggtcaatc cccaccgagc tggcggcgcc
     2461 gtcggcgacg ctccacgaca gccggaccgc ggcggggccg ccggccggcc ccgccgccgc
     2521 gtcgaagctc gcgatcgcga tcgccaccgt cgcgcaggcc gaggtgcccc ccacgagcac
     2581 gtcgtcgatc caccagccca gaccctcgtt ggggggctcg gagcggaacc ggaacgcgaa
     2641 ctggacgatt cccgaatagc ccgtgagcgg cacgtcgacg cgcgtgaacg tggtctgggt
     2701 cccgctgaac cggtcccccg tgttgtagcc gcccaccggc tcgagcggcg tccacgtggc
     2761 gccggaattg gtggagatct cgaccgtcag gccgtcgaag gcgaactcgc tgtcgtagcg
     2821 ctgccagaag gagagcgtat cgctggtgcc cgtgagccgg accggctggc tcacgagccg
     2881 cgcgtcctgc gacggcgcgt agtgtccgat gaagccgaac ggccccagtc tccaggccca
     2941 gctcccgccg ggggtgtggt tgacgcccgc ttcgcggtgc cattcgctga cgcccccgca
     3001 gccgagcggc accgagaccc accggcgcgc ggtggcgttc tcgaagtcct cgcagatgcc
     3061 gctgcgctgt ccgatcagga tctgcacgct gtcggcgaga acgaggcccg cggcgtcgga
     3121 gaccgcgatg gtgagattgc agccgcgcgg atcgggggtg gggttgatga cggcgaagat
     3181 caccgaaccc gtgtccgagg ctgccccgcc cagaaccccg tagtagaccg agtcggccag
     3241 gagccccgcg tagggctcgg tgacggccac ccgcgccgtg aggacgccgg tcggcgtggt
     3301 gtccaggttc tgcaggggga tgtggatccg gacggtctcc cccggttcga cgagcccgtt
     3361 accgttgtta ttgttcccga ggaagggaga gtcgtccacc gtcacggcgt tccggcgcac
     3421 cgacgtcccg ctcacgaaga aatcggccgt cacgtcccgg ggctccgctc ccgtttcggc
     3481 gatgttccgg atcgccacct gcgagatgcc gccgcagtag gagtcgctgt tgggacagct
     3541 gttcgcgtcg aacgcggcgc gcgcgcccgc cccgggccag ggatcgtagg cgtcgccccg
     3601 gtttccgacc acgatcaccg acccctcgca cgccggctcg ctggccgccg acccgcgcgc
     3661 cagatccagg ttctggaccc cggtgaagcc cgacgcggcc tcgatgccgc ccccttcctc
     3721 cacgtccacc tgcttatggg acgccgtgtt gttcccgatg atcccggtgt ccacgtggta
     3781 gatcatgagg ccgtgcgccg cggtcgaatc ccgctccatc gtggatttca cgagcgcggc
     3841 gtcgaagccg atcggctggc ggttctccac gaggaagtac tcgccggctt cgaccccgtt
     3901 ggtccagagg cggaacgcgc gtcccgaggt ttccaccgga ggaatcgtca cgccggcgct
     3961 gtcgcgggtc acccaggtgg gctggagcca gccgagcgct tccttggacc aggccgagag
     4021 gtgcgcgggg cagctcccca ccgtctggtc cggaaggtgg ttgaagagtc ccgaggccat
     4081 cagatcccac tcgccgagcc cctcgttggc tcccggaagg ccgctcgtgt cgtagaggtc
     4141 gggaagtccg agcacgtgtc cgaactcatg gcagaagacg ccgatcgtca tcaacgcgcc
     4201 cttcgtggtc cagggcgtcg tgaggaacca ctcctccggc tccgtcgtgt agtcgtagac
     4261 gatcacgccg tccgccgacg tccctccgcg aggccccggc acgttccact tgtgggacca
     4321 gatgtccgag gtggggtcgc ccgtttcctc ggcgcccgga cccgcgtgga cgatgaagat
     4381 tccgtccacc tcgccgtcgt cgtcgccgtc gtgcgggatg ccgtcgggcc cgtcattgtc
     4441 gaactgggtg aagtccacgt cgggctccgc gagacggacg gcgtcgagcg ccatcacctg
     4501 cgcgttcagg gggcccgggc cctggcctcc gtccgtgccc acgtagtcgg tataggggcg
     4561 cggggccgtg taccaccggg tcacgacgcc gtcgacgtcg aacgcgccgc ccgacacctc
     4621 gttgtagtag tcgcgaaagc tcccgccggg acgggttccc ttggagaaga ggagatcggc
     4681 gaagtcggcc ggcgtgtggt tcagcgtgtc ggcggggtga tcggagaact ggagcaggat
     4741 gacgaggacc ttgtacttgc tgcccacggt cgtccggacc tgggacgggg cgttcagccc
     4801 gggctgcctc agggggatcc gcggggcctg gaacgccgtg gcgcgccggc ctggagccga
     4861 agggtagagc ggtggagcgg cggatacccg gccggccggc atgagcaggc aggccagcgc
     4921 gatcccgaag acggcgcgca aacgacgaac tccctccggc agggatgggt cgtggcgccg
     4981 atcagggttt gacggaagca acctccgcgc gcccgaagcc ggcgcgctcc tgcttccccc
     5041 agccgccacg tcccaagagg atatcaacca tggcgcccag gcggaagaag agggtgagga
     5101 tgcggtaggt gaagttctcg atcaccccga tggcgatcat cagggcgaag tcgccccagg
     5161 agcggtagag cccgaacgac agttcctcca ggaagaccgc ggagatcgac aggaaggccc
     5221 ccgaggtcac cgacaggaag agaaacgccc agaagtagag gggggacagg aaacccatga
     5281 agtaggcgat cgggagcacg atgtagccga tcacctcgac gatcggcccg agcagctcga
     5341 acaggaagta gtagggcatt ccgagccagc ggagcgaccc cgacttcttc tcccagaaga
     5401 tcgagcggtg gagccagagc gagtggatca gcccccggtg ccagcggccc ctctggcgcg
     5461 ccagcgtgcg caggccctgc ggcacctcgg tccagcagat cgggtcgggc aggaagacga
     5521 ccgaataggg ccgcccctgc tcgcgcatcg cgtggtggag gcgcaggacc agctccatgt
     5581 cctcggtcac gacgtccgag cggtagcccc ccacttcgac gacgacgtcc ttccggaaca
     5641 ggctgaacac gcccgaaagg atgaggaggt tgttcatggc cgaaagcccg aggcggctcg
     5701 cgacgaaagc gcgcaggtat tcgaccacct ggaacgcggc caggacgttc tgcgggaggt
     5761 gcggatccac cacgcggccg cgttccaccg tgcagccgtt ggcgacgcgg acctggccgc
     5821 ccaccgcgat cacgcgatcg ggatcttcca ggaacggacg caccacgcgc aggagcgcgt
     5881 tctcctggaa cacggagtcg gcgtcgatgg tgcagaagag cggatatcgt gagacgttga
     5941 tgcccgcgtt cagggcggcg ctcttgcccg cgtgcgcctg gtccaccacg accaggttcg
     6001 ggatcgtcgg ggagcggtag atgcctcgca ggatcgcgtc ggtcgggatg ctccggcgga
     6061 cgatcgcctc gcggcgcacc agcccgaatt cgcgggtcag ccgctccagc gttccgtcgg
     6121 tggatccgtc gttgatgacg atcacctcgt gctgcggata gtcgagggag agcaccgatc
     6181 gcaccgcttc gaccaccgtg gtctcctcgt tgtaggcggg gacgagcacg ctgatcggga
     6241 aggtgaagtg cgacttggcc accgcttcgt aatcggcgta gaagttctgg gccagccgcc
     6301 ggcgcatgtc gaagaaggag acgatgagca gcgtgaagag cacgagctgg agcgccgtga
     6361 agtagacgag cacgatgatg ctcacgacgt tgagcgccgg gaagagccag gtcggagcgt
     6421 cgatcgcgtt catgtcagcc tcccgtcgcg gcccgctggg gcgggaccgc gggcacgccc
     6481 agggtggcca gtccttccgt ggcgcagtcg cgcgcgaagc ggtcgttcga ttgcagggcc
     6541 gagaggagcg ccggcttggc cggctcgccc agcagcacca gggcgtccag cgcgttcttg
     6601 cgcacccacc aggatcggtc ctcgagcatc cggcccagcg cccccgccat cgtggggtcg
     6661 ccgatcttgc ccagcgcctc ggcggcgtgc gcgcgcacga accacaccgg gtcgcggagc
     6721 gcggcttcga ccatcgcggc cgtggagcga tcggcgatcg cgcccaaggc ccaggccgcg
     6781 gacgaacgga cgtccggctc gggatcgagg agcgcgtcgc ccagcgagcg ggccgcgcgt
     6841 ccgtccttga tctcgcccag gatgcgggcg gcccagaagc gggccttcgg ggtgccgtcg
     6901 cggagcgcgc gaatcagcag cggtgcggtg cgggcgccca tctcgatgca gatcgcggca
     6961 atgcgcgccg agaccgactg ctcgtgcttg cccagaagcc cgaccagcgc ctcctcggcg
     7021 gcttgcagct tcatcttgcc gagggcgcgg gcggcgacgt tgcgcacgtc gggatccctg
     7081 tcctccagcg cggccagcag ggcgggcacc gatgccggca ccttgagctc gcccaggagc
     7141 cggagcgcat gggcgcgcga ccacttgagg gggctcttcg tctcgcggag gtaatgggcg
     7201 attaccccct gctgctcgag ggagcggatc acgtcgaggc ggagggatag ctccagctgg
     7261 ctcacctcct ccgcggcgag ctgcgaccag cggccggtga gcccgttggc gatctcgcga
     7321 taggccgcat cgcgctgctc tccgccggcc agcaagagga agctctggcg gagccggtcg
     7381 aggaagcggc gccgcgaatc gtcctcgcgg ttggagtgga tcttgttcac gacggccgcc
     7441 atggtcgtga gcgccaggat cccgagcagg agtcccagcg tgatcaccag cgcctggtag
     7501 tcgatcatgc gatccccctc gccgcgctca ccatcggacg gctcccgagg ccccgacgaa
     7561 ccaggtctcg taggcgttgt tgtccctcga gtaggagccc tcgaggccga gcgggaacga
     7621 cccgcccgcg accaggttca cccagcccga ccacgtgctc gtgtagatgt tgtcgaagtt
     7681 ggacgagagc accgccgaga actggtagcg cgccccccag tcgagtgatg ccctctcgga
     7741 gtatcccgtg agggccaggc ccgcggcgtg gcgcgtgctc ttcaacggcg tgaagtagga
     7801 cgccgacgac gagtcgaaat cggtgaacga gatgccgtag tcggcgctca ggcccgggcg
     7861 ggccaggcgc agccggtacg cggcgccggc gcgcagcgtg ctccggtcgt tctcgtccga
     7921 ataggatccg tgctcggcgc ccgcgctcag cgtggcgcgc gcgttccact ggtaggccag
     7981 ttcgcccgcc accacggtct gccgccccgc ccggatctcc ggcagtccgg tgtcgtcctt
     8041 gatcggcacg tagtcgcggc ggaccgaagc gctgggcgtc cagcggcccg cgaatcccca
     8101 cgcgcgcgcg ttccagccaa agaagcggtc gtcgccgcgg agggggtaga tcacgttggg
     8161 gctcccggcc tggatcgcgc ggctctccac gccggcgctc agcaggaagg gatagccgcg
     8221 ggtgagcgcg gcatcggcgc gcagcacggt catgtccaga tcgtagttga gcgtcgtccc
     8281 caggaccggg gggcccaggc ggcgctcgaa ctgggccagg cgcgacacgc gcaccgtgac
     8341 ggtggccgcg cgcgccaggg tcgcgcgcgc ctgcgcgaag gggcccgtgc tctcgatcgc
     8401 gaaatcggga gcggcggcca cgtactggcg ctcgtgcgcc gtggtgaggc cggcgccgat
     8461 cgaggggccg agcgcgtccc gtacggccct gcgctcttgc cccatcgctt cggtgtcggc
     8521 gctcccgtgc gccgcgcgct cgaggaagcg gtccgacgac gatggggcgc cgcgccagcg
     8581 ggcgacggtg gcatagccga gccacgcgtc gccgtacgcg ggattcgagc tcaggatgcc
     8641 gcggtattcc gtggaggcgc cggggaggtc tccggcccag gagagagcgc gcgcgtagcc
     8701 cacgcggctt tcgagatcgg tggagtcggc gcggaccgcg cggcggaact cacggagcga
     8761 agcctcggag cgccccgacc aggcgagcac ccgcgcgtat cccgcgcggt cgaacgcatc
     8821 gtcgggatgc gcggcgagga tcttccggta ttcggcgatc gattcgtcgt aatgccggcc
     8881 ccaggcgagc acgcgggcga gcagggactg cgcgtcggcg tcctccggag cctgggccag
     8941 gcgcgcgcgc aggatgcgcg cggcctcgtc gaacttcttc gcgtcggcca gggcgcgggc
     9001 ttcggccagg gccgactcgg aacgggacga atcctccgcc gcgacgcgct ccagcgtctt
     9061 cgcgggagag agggcattgg ggtcgggatc ggcgagcgcg ggcgagacgg acagtgaagc
     9121 gagggcggac gcggccagga atgccgccca cgccgcggcg aggcgcggcc tagcggagcg
     9181 cgtcctggtt ccggttggct tccgactcac gggattccat tccctcgaag gcttgacgga
     9241 cgagggccgc cgattccttg gccctttccc gggttatcgg ctgctcccag ccgttccaaa
     9301 gtagagatac gtgggcgggc gtggcgaccc gaacctcctg accggtctcg gcttgggtcg
     9361 ttcccggcgg tcccaggaac tggctgtcgc ccggtcccgc gggtcgtgtc ttgggtccgg
     9421 aagggccata ggagtccgcc gagaaaccgc gcgccgaggt agcccaggcc aggaatccga
     9481 tccccgccgt gggcgtggcc tcgacggcgc tcgtttcagg gatgtcgtcc aggacggagc
     9541 ggctggaagg gtcggagacg ttgagaagac cccagggaat ggcgacttcg atcacgccgc
     9601 gcacgggatc ggcgcaccac tccgagtgcg gatcgtacgc cacgctcgag tcgtctcggg
     9661 cgccccccgc cggggagcgc tccgcgccat agccgagact cccccagtcg aggtgtctcg
     9721 cgggatagac cgtgccattt cgcgccacgc gctcgcggtt cgtctcgatc aggagcggaa
     9781 cgtacgctcc gtccgcgttc gccacgggtc ggaacggagc gcgattccga acgaagacgc
     9841 cgtcctcgag aagacgcgac catcccgacc agttcatgct tcgatcgatg aacagctccg
     9901 cgccgggccg gtcgcgcagg ttgcgctgca gtgccgacgc gggttcgatc accagctgga
     9961 actctgcgcc gcgcgaccag acggcgcgca ggggggcggg gagccgcgtg tcgccccgcg
    10021 cgggatcgag cacgtcgatc gaggcgccga gggccgccgg agtttccttg gtcggatcca
    10081 cgtcgaatcg gagatagagg cggttcgcgt cgctcgtcgc gtagaacgcc ttgagccatc
    10141 ggcccttcgg gtcgctcgcg tagggagcga tgccccgcca gtccgacgtc gcgccgtcca
    10201 cccggatccg gggcggcgga tcgaacgcga tcaggccgta gttctcctcg ggatcgagca
    10261 ggttgtgcca gagcgggtcg cggtcgcggg gcgcttccat tctcgcgacc agccagttca
    10321 ccttgaacca ctcgtcgaag agcgcgaaga gaagcgaacc ggagcacccc gcgtcggcga
    10381 tgtcctcgag cagccggacg tcgcgctccc cctgctcgcg ctccgaggcg ccgccatggt
    10441 cgattccctg cggctgctgg tgcgcgatcc cccggctcgt cggcacgccg tactccccga
    10501 tcatcagcgg aatgccgcgc gtgtgcgcct tgagatcccg gaggtagccg gcgtaggagc
    10561 aggcgccgtg gcggtcgcgc acggccgcgt agccagggtc ggtgttcatg aagtccgggt
    10621 agtagggata gacgtggtag ttcgcgaagt agccgcggaa cgacgggtcg accgccgcgt
    10681 tcttcagggg gcggatgcgc aggggatcga ccgaataggc gtcttcgtcg tgctcggcct
    10741 cgcgcccccc gcgctcggtc tccgtcgggt ggcgcatcac gtcgagcgtc ggccagttca
    10801 cgaacgcgac ggcgtgcgcc gtgccgtagc gcgaggcctc gtacgaggcg gaggtctcgc
    10861 acgcgcgcgc gagccaggtc tccatgggcg tgcccgcctc cacggcgaag aaggcgccgc
    10921 ggaacgaggt catcccggga tgcttccgct cggtggcacg cacggcgtag ggctcccatt
    10981 cgcgtcccag cagccagccc gcgatccatg gcgagacgtc ggcgtcataa cggcccgagg
    11041 catgccccgg ccgcggcgcg acgagcgcgt ttccgtgcac ggcgtcgatg gcggaccgga
    11101 tcgcggcgtc gaacgcgccc gtgaaggaag cctcccagaa atcgtccccc tcgggcagct
    11161 cggtccagac ctcctggaag agccagatcg gacggtcggg atgcgccgcg ttctcctcgc
    11221 gcagcgcctg gtagaacgcg ggcggatgga ccgcgtacac ccggatcgcg ttcgcgtgca
    11281 gctcgcgcgc gaatcgaagg tagcgacgat agtcgtcctt ggtgaaggcg aactcgccgg
    11341 ggaagtgccc cggcatcccc gcgcccaggt tcacgccgcg gaaaaagacc ggacgcgccg
    11401 agccgttgcg gacgatctcg aaccgatcgc cggccgcgcg cacgtaggcc gcggccggac
    11461 cgcgcgcggc ggaaatcgcc ggagcggttc gccccggcac ggcgtgcgcc gacgcgtgcg
    11521 ccgatatcgc gggcggagcg gcccacgacg cggccgtgac ccacagccag acgaaggcgt
    11581 cgatgagaag gggcatgcgt gtcctccttc cgcgcgttat cggcaggaag aaggttcggc
    11641 tggaggatgc ttcagcgagg cgcccggggc cagcgaaacg ttgcgtccca ggcggggggc
    11701 tgttaggaat tctgtgaaaa cggccctacc caaggcagac gatatgactc tcaagcggat
    11761 ggacaacata ggcatcgtgg ttgaatccct cgatgccgtc atctcattct tctccgagct
    11821 aggcctgaag ctcgaagggc gggccatgat cgaaggagag tgggccgggc gcgtcaccgg
    11881 actgggatcc caacgcgtcg agatcgccat gctggtcacc ccggacgggc acggtcggct
    11941 cgagctttcg cgattcctaa gcccgtccgt cgtcgccgac caccggaatg cgccggtgaa
    12001 cgctctcggc tacctgcgcg tcatgtttac ggtcgacgac atcgacgaaa cgctcgccag
    12061 gctccgcaag cgtggcgcgc agctcgtcgg ggaagtggtt cagtacgaat cggcgtatcg
    12121 actttgctac atccgcggcc ccgaagggct tctcatcggg ctcgccgagg agctcaggta
    12181 gggcgactcc ccaaaacgat cggttcggca acatcccgtg gcgcggcgcc gatcgcgtga
    12241 tgccgacatc gcggcgcgtt tcggagtggg cgtcggggcg tggatcatgg gcgcaacatg
    12301 ttcggcccgg ttcgtggtcc ctggccggac gacgcctggc gcggctggtg ggggaagaac
    12361 ccgccgtatc acggaccggt attcgtgctg acgcatcacg cccgcccgcc cgtcccgatg
    12421 gaaggcggca cggtgttcca tttcgtcacg ggcggcattc gcgaggcgct ggaacgggcg
    12481 atggcggcag ccgagggcaa ggacgtggag atcggcggcg gggtggcgac ggtccgccag
    12541 tatctcgaag cgaggctcat cgacgagctg cacctggcgg tcgcgcccag gctgctcggg
    12601 tccggcgagc atctcctgtg cggcctcgat ctcacggctc tgggctacga gtgcacgacc
    12661 cacgtgccga ccgccgccgc gacgcacgtc gtactcacga gacgccggtg acgcttccgg
    12721 gcgagggcta cggaaagatc gcgcggagcg cggcttcgtg gttctcgaag gaatcgagga
    12781 tggcgtccgg cgcggactcg gccagctcgg atcgcgagac ctgtccggtg gccaccacga
    12841 ccgctcgggc gccgatcgcg cgcgcgcagg agacgtcgcg cggcgtatcg cccacgaccg
    12901 tcgtgtccgc gccgctgaag acgcgtccgg tcagggcgcg cgccttctcc agggcgatct
    12961 gggccagcac ggagcggtcc tcgtggtcgt cgccgtaggc gccgaaacgg aaatagggct
    13021 ccaggttcca gcggcgcagc ttgagctcgg cgccgcggag gatgttcccg gtcaggaggc
    13081 ccaccatcac gtcgtccagc ttgttcagac gctcgagcag ctcgcgcacg ccggggaaga
    13141 ggcgggcccc gtcgtggttg ggcatctcca cgtgcaggtg ttcgacgtag cggcggaggc
    13201 attccttgcg ccgggagtgg atcacgtcgt cgtggacccc ggcgagccgc atgatctcgg
    13261 tgacgcactg cggatcggtc atgcccgcga acttgaagtc gttgatcggg ccggtggtgg
    13321 cgtaggtctc ccggagggcg cgcgcgaacg cgatcttggg cgcgagtccc tcgcggagca
    13381 gggtgccgtc gatgtcgaac aggacgaggc gcatggatcg ggaccggcgc tggaagcggg
    13441 tgcgtttgtc ggtttcgatg gcggacacgt tcgatcggag agaccgatcg gacctctcgc
    13501 ggcgattgta ccacaggccg cgggcggtgc tcccggacct aggcccgcgc ccccgccgcc
    13561 gctccggcgg gcgctcccgg cgcgcccgcg tgcggtccct cgaagagctt cggattggtc
    13621 gagcgctcgg cggccgcttc gcgcgtgatc ttccccgcga tcacgaggtc cttgagcgcc
    13681 tggtccagag tctgcatccc gtgctgcgcc ccgacctgca tgacgctctg gatctgcgtc
    13741 gtcttgtcct cccgcaccag gttccggagg gcgggaacgc cgaccaggat ctcgagcgcg
    13801 gccacccgtc ccttgccgtc cgccgtcggg atcagggtct gcgcgatgac gccttccagg
    13861 gactcgctca ccatcgtccg cacctgggac tggcggtcgg cgggaaagat gtcgatgagc
    13921 cggtcgatcg tcttcgcggc ggagttcgtg tggagggtgc cgaagacgag atgccccgtc
    13981 tcggcggccg tgatcgcgag cgccgtggtc tcgaggtcgc ggagctcgcc cacgagcagg
    14041 atgtccggat cctcgcgcag ggcgcttcgg agcgcggccg cgaaggagtc ggtgtgggcg
    14101 ccgatctccc gctggttgat gaggcagctc ttggactcgt gcacgaactc gatcgggtct
    14161 tcgatcgtga tgatgtgccc cgcgcggctc gcgttcacga agtcgatcat cgcggcgagc
    14221 gtcgtcgact tgccgctgcc ggtcggtccg gtcaccagga ccagtccccg ctcccggagc
    14281 gccagctcgc ggaggatctt gggcgtgccc agctcctcca gggtgcggat cttggtcgcg
    14341 atcacccgga agacgccgcc gatcccgcgt cgctgctcga agatgttggc gcgaaagcgt
    14401 gagagcccct cgatctcgaa cgcgaagtcg atctcgcgct gcccctcgaa cagcttccgc
    14461 tggccgtcgt tcatcagggt gtacaggagc gcgcgcgcgt cggggtcgga gagcgcgggt
    14521 gactgcgagc ggaccatgtc gccgcgcagg cggatcatcg ggggcatgcc ggaggagata
    14581 tggaggtccg aggcgccgga ctcgaccgtc gcctggagca gggtcttcac gtcgagcggc
    14641 atcagcggcc tccctccttc gcgagcgcat cccgtttctc atcggagagg atcgtgacct
    14701 gcaggcttcc cgcctggatt tcggcgcgcg acggatccac ccatacggcg gaaccctcgc
    14761 ggcgggcggc gggaagggcg atcaaagcga aggggcggtc gcgcagggcg gcgcggccgg
    14821 cccggcttcg ggtcagatgg gcgatgagcg ccttggggtc gagccagtcg gtgcgaacga
    14881 agacgaggcg tcccacggcg gccccgtgga gcagcgtctc gacctgctcg ccgtccacca
    14941 cgccgtccag cgcgacgacg tcggcgccca gcccgacggc aagctgctcc cagcgcgccg
    15001 cgtcggtcgc ggggatgcgg atcgtcccgg gagggtaggg gagcagatgg gccggctcga
    15061 acaccagggt ccggcggtcg gcgcgcgcgg ccagcgcggc gagcgaggcc agcaccaggg
    15121 atccaccggc gcgcggaggc ccggtcacga cgatcagccc ctcgggaccg tcgatgaatt
    15181 cgcggatctc ggcctcgccg atcggggaga gcccgagcag cgagaggtct ggcgcttcca
    15241 ccggtgcggg ggccaggcgg aggaccgtgg cgacccccgc gtcggtccgg ctgtggcagg
    15301 cggagaccgc aacgcggtcg gggcccagct ccacgatggc ggcgccccag gacgaggcgc
    15361 gcccgtcgtc cacgtcggga atgcccagca cgccgagccg cgcgcgcagg tagagcgagg
    15421 tttccggctg ctccggagtc tgcgtcacga gccccgcgtc ggtgcggtac gccaccgtga
    15481 ggtcggagcc cgcggggatg aaatggattt cggaggcgcg cgcctgcacg gccgcgtgga
    15541 gatggtaggc caggaagttg gtgcccgcgc ggtcccagac gatgtcgcgg cggctggtgg
    15601 ggggcggcgc cgcgtgcggc gatccggatt cgggcggtcc atagaccgag tcgagcacgc
    15661 gccggatgga ggccggaccg ccgatcacga gcgaggcggg gatgcccgcg gccgccttca
    15721 gttcggccac ggcgtccacg tcggtcgggt cggccatcgc gaacgtgatc tcctcttcgg
    15781 tcccgaagag aggcacggcc tgaatgcgcc ggaggagccc ctcggggaag cgcttcacca
    15841 tctccggatc caccgaggag gggtgcacgt ccgcatacgg gaagccgagc tgccgcgaga
    15901 gcgcccagcc gatgttctcc tcggtcgtga agcccagctc gacgagcgtc tcgccgatca
    15961 ggaggccggt gtcgcgctgg cgcgcgagcg cctgggccac ctgctcgtcg gtgacgatcc
    16021 cgcactcgac caggatcgag gtcagcgtgc gcgtggaatg cggaaaagga gccacggagg
    16081 gacacctcgc tttcggtcga agagagcgtt cgggaaaccg atcggaggtg gatagggcaa
    16141 tccatgtgcc gcgtgcgcct ccggcgcacg cggaaggcgg cagggggtgg tccagcggcg
    16201 ctcagccgag gcaaagtgac tgtactggat ggggttactc tccgattccg tcctgcgagg
    16261 gcctcggggc atctaaaccg cggactctcc cccgccggac tccccgcggc tcaatccttg
    16321 ccgcccgggg agcctccggt tagattctcg gcgaacttgc gttcctgagg gcagggggga
    16381 ggagagggag gccatggcgc aagcattgaa gaaagactcg aaaaaggccg cgtccacgcc
    16441 ggcggtccgc aaaccggcgc cgctcaaggt gccgaagggc gtgaagaaga tcgtccacct
    16501 cacggccgcc gatctcgccg agaagcagcg cgagatctcc gtcagcgaat tcttcaccaa
    16561 gaaccggcac ctcctcggtt tcgacagccc cgccaaagcg ctcctcacca ccgtgaagga
    16621 agccgtcgac aacgcgctcg acgcatgcga ggaggcgggg atccttcccg acatcctgat
    16681 cgagatccag gagatctccg agacgcgcta ccgcatcgcc gtccaggaca acggcccggg
    16741 catcgtgaag gcgcaggtgc cgcgcatctt cggccagctc ctgtacgggt cgaagttcca
    16801 ccggctgcgc cagtcccgcg gacagcaggg gatcggcatc tcggcggccg ggatgtacgg
    16861 ccagctcacg accggaaagc cgatcaccgt gatctcgcgg ccgggccagg gaaagccggc
    16921 gcacaagttc gacatccaga tcgacacgcg cgcgaaccgc cccgtcgtgg tggccgatca
    16981 cgaagtggaa tggatgccgg cgcacggcac gcgcgtcgag ctggagatcg aggcgaccta
    17041 caagaagggg cgccactcgg tggacggata cgtggagcag accgcgatgg cgaacccgca
    17101 cgcgcggatg acctacgtcg cgcccaatgg cgagcggctg gagctggagc gcgtcgccga
    17161 ggagatgccc aaggcgccgc tcgagatcaa gccccacccg cacggcgtgg agctgggcat
    17221 tctcctcaag atgctgcacg agaccaaggc caagaacctc ggagccttcc tgagcgggga
    17281 gttctcgcgc gtcggcccga ccgtcgccga cgagatcgcg cgcgcggcgg gtctcccggt
    17341 cagctattcg ccgcacaagg cggcgcgcga ccaggccgag gcgctctaca acgcgatcca
    17401 ggcgaccaag ctgatggcgc cgccctcgaa cgtgctctcg ccgatcggcg aggagctcct
    17461 gctcgaaggg ctgaagaagc agatcgacgc ccgcttctac gcggccacgt cgcggtcgcc
    17521 gtccgtctac cgcggaaacc ccttcctcgt ggaggccgcg ctcgcctacg gcgcggagag
    17581 tttgcccgcc gacgagctga tcacgatgct ccgcttcgcc aaccgcgtgc cgctcctcta
    17641 ccagcagtcg gcgtgcgcgg tcacgaaggc ggtcgtcggg acgaactgga aggcgtacgg
    17701 gctgcagcag tccaagggcg cgcttcccgc ggcgccgatg atcctcatgg tgcacatcgc
    17761 gtcggtctgg gtgcccttca cgtcggagag caaggagtcg gtcgcgtcct atccggaaat
    17821 cgcgaaggag atccggctcg cgctgcagga cgtcgggcgg aagctcggga tctacatccg
    17881 gcgcggactg cgcgaggcgg acgccgagaa gaagcgctcc tacatcgaga aatacattcc
    17941 ccacatcggc atcgcgctcc aggagatcct cgcgatctcc gacaaggagc gcgacaaggt
    18001 gtgcgacacc ttgaaggaca ccctcgaacg gagccgacag tgagcgacaa ggtcgtcccc
    18061 aagatcaaga aggaagccaa ggcggtcgag gagtcgatcc tcaagaagaa gaagccggtc
    18121 ttgaagttcc ccgtgcgctc gctcgccaac gtgcgctatt tggagaagga ggggtacttc
    18181 gagatccggg ggaagaagaa ggagcgcacg ctcaccgtga acacggtgaa gtcgttcgcg
    18241 cagaccctcc ggatgatggc gctctccaag tcgctcgtcg agaccgacga catcgcgacc
    18301 aagcgggaag cgtactacgt gtcgaagaac tggggcgacg cgcgcttcct ggagcagacc
    18361 gagtcggaca cggtgatgga cgacgttgag gcgcttttcg aggtgaatcg cgagcagatg
    18421 ggcttcgtcc ccgaggagaa ggggggcgac gtctccggcg cactgaccgt ggtggacaag
    18481 gatccggata cgggaaagtc gctgcgcatc gactgcacca agttcgggag cggcgcctac
    18541 tccatcccga tctccgtcga agacctggaa ttcgagacca aggcccagtt catcctggtg
    18601 atcgagaccg cgggcatgtt ccagcggctg gtgaagcacg gctacgccaa gaaggcgaac
    18661 tgcatcctgg tctcgatggg gggcgtgccg acgcgcgcct gccgccgctt catccgccgc
    18721 ctggccgacg cgaagaagat cccggtctac gtgttcgtgg acggcgatcc ctacgggatc
    18781 acgaacatct accgcacgtt caaggtgggc tcgggaaacg cggcgcacat caacgagttc
    18841 ttctgcgtac cgcaggcccg gttcctgggc atcacgccgc aggacatcct ggactacaag
    18901 ctgcccacgc acccgctgaa ggaagtggac gtgaagcgcg ccaaggacgc catcaagaac
    18961 gacccgttct tccatcacca caaggcgtgg gccacggcca tggagaagct gatctccatg
    19021 ggcgtacgcg ccgagcagca ggcgctggcc gcgcacggcc tgaactacgt gatggacacc
    19081 taccttccgg agaagattgc gcaccccgag cggttcctgc cgtagccgtt gggctccgac
    19141 ctccaggccg tccgtgaagc gatcgaggcg gtcgaccgcg agatcctcgc cgcgctgaag
    19201 cggcgcatgg atctggtcga gcaggtcgcg cgcgccaaga tcgagagcgc gacccccttc
    19261 cgcgaccgcc agcgcgaaga gcagatcatc ggccggatcc gccaggccgc ggccgagctg
    19321 ggcctggacg cgcacgagat cgagcggctc taccgggaca tcatggagat gtcggtggcc
    19381 caccagcagg cgctggtgcg ctcgctggaa ggggtgcccc tgcgtgtcgc gtaccagggc
    19441 gtggagggct cgttcagcca cctgacggcg cagcgccgct acgcggggcg cgagggcggc
    19501 gtgctgttga cgggccacga caccttccgc ggcgccgtgg gcgccgtgcg cgacggctcc
    19561 gccgacttcg ccctgatgcc gatcgagaac acgacggccg gcagcgtgca cgagacctac
    19621 gacctgctcg ccgaaggggg cgtgacgatc accgccgagg tggtgagccg cgtggagcac
    19681 tgcctgctcg cgcttcccgg cgcgaagctg gagtcgctgc gcaccgtcat ctcccacccg
    19741 caggcgctcg cgcagtgcga ggccttcctc cgtacgattc cctgggccgc gacccggacc
    19801 gagttcgata ccgcgggctc cgcgcggaag gtgcgcgagg ggaacgatcc gacggtcgcc
    19861 gccatcgcga gcgagtcggc ggcccgcctc tacggattgg aagtgctgcg gcagggcatc
    19921 cagacgcagg aggggaacta cacccgcttc gtcgagatcg cgcgcgaggc cgtggcgtgt
    19981 ccgcccgggg cggcgtgcaa gacgtccctg cagatcgcgc tggcgcacaa gcccggtgct
    20041 ttgggggaag tgctcgcgcg gttcggggcg cgcggcgtca atctcacgcg gatcgagtcg
    20101 cgtccggtgc tgggatcccc ctggcagtat cgcttctacc tcgacctcga aggccacgcg
    20161 gcgtcggagc cggtgcgcgc ggcgctcgag gagctgaaag cggtcacgac cgaggtgcgg
    20221 gtgctgggga cctatccccg agcctcggag ccctgactcc cggccgggct cacgcgcccg
    20281 cgaggagccc ctgtcgaaac gcctccagcc gggacaggac gtctcgcgga ttcgcgcgca
    20341 ggagcggcgg cgagactcga accagcgtcg ccagtcccgc cccgccgagc agcaaccgaa
    20401 cgaagccctc gatgctcttc atttcgactt catccgcgat ccttccgaca tcttttccca
    20461 gcaagtagga gccggcgtcc tcacgggtga cgccttgggc ttcgtcccag cggcgggtac
    20521 cggcgtcgtc gaggtagacg tccatcaccg tcgcgaagtc gtccacgtcc cgctcccgtt
    20581 ctcgaggccg gtcctggtat gcgaccgact tcatgaggat caccgccggc accgatgccg
    20641 accgtatcgt gacgccgcga gccacgttca gcggattcgt atgttcgaac gcgtgcctca
    20701 ttcccacgag ggtcatccgt cgcccgctgg cgggccagac caggaacccc tgttttcgag
    20761 aggacgcatc gaggggaatg gcgtgcacca gggttccact ggcgctgcgc cactcgaatt
    20821 cggtcctgcc ggtctgggtc cacccgggca atcgcgccag cgcggcgttg gcctcgtcca
    20881 gagggagagc gatcgtgata tcgacatcgt tcgtctgccg gcggaatcgg ggcagggcgc
    20941 acgcgagcgc cagcgcgccg atgaggctca gatcgaccga agatcccagc ttggcgagat
    21001 ctcggaacgt ccccagatga ctctccgaaa ggcggatcat gacaggtgct ggaggtatcg
    21061 ctcccggaca cggctggccg cgtcccgggc ccgttcctcc cccgtggcca ggagctccga
    21121 atagatgagg agcgggtgca ccacgttcgg ctgggtgcct tggaatgcga gtggacccgg
    21181 aacgccgagg aacgtgatcg agccctctcg tgccggagcc aggcccaatc gcttcgccgc
    21241 ggtcgagggc ggggcgtcga agtggatgac cgttgccgtt ccacgatagt agtgaaggag
    21301 gcgatgctcg gccgcggccc cgccccaggc ccatcggatc gtcttcgacc cctccacgaa
    21361 ccgatcctcg ccgtgtgtgg aaagctgggc gcccaggaac cggtcgagct cgtccgggtc
    21421 ttcgaacctc gcctcgtacc gtccgagcac gagccggggg cggagccgat cgacgtagcc
    21481 ggtcagccac cgctggagca gcgcgtccgg gcgcagcagg atgcgatcgc ggcccgtggg
    21541 gccgatgacg ccttcacgct cgagccggtt caggaccgat gaagccgccg tcttgctggc
    21601 accggatgcc gatgcgatct cccgcaccgg ccgcgtcacc agctccggac gcgccagcaa
    21661 ggcgaactgg atcctggtcc ccgcgcgccc cgttccctga gactcccacg ccggcgtcga
    21721 tcgttttcgg ccttcgatgt aggccacgtg cccctcatcg atcgagagat ggcagttgcc
    21781 gctcgcgtcg acgaaattga ttcgatgctc gcgcaggtat cggctgagct tgggtccgac
    21841 gtggttggcg agaaggatcc aatcctgctc cgagcctttc cgctggtcca tccagtggtc
    21901 gagggcgatt cggctgaggg gatcggaccg cctgagctcg accgcgagaa tccgctttcg
    21961 tccaccgacg gtgatctcaa tacaggtatc gggctgggtc atggcggggc gccggacctc
    22021 tcgcggcttc gtgaggatag ggagctcggc gagtcggtcc aggaagcggc ccaggtcggg
    22081 atgttgcttc atggcccaca ttgtggcatg tcccaaaaac taggacaaac aaaaaagccg
    22141 gacaaacggc actcggaaga actgaggggc tgttcggtgt aggacctgac gggctccctg
    22201 tttttggtac attggtccga ctcgcgatcc accttccgac accgcacctt gaggccatcc
    22261 catgagacct gttccaggca tcgccgcggc gctgctcgcg gccacgctcc tctccactcc
    22321 gatcgcggct tcgacctcct tcgcggcggg gaagatcccc atcacgaagc tcgccgacct
    22381 gccgccgcac agctacaaga tccccgacaa gcccagcgcg ctcgtgacga acaaggccgc
    22441 gatcctcgag ctggccggcg ccgtcgagaa ggacatgaag gccgacctgg acaagtacga
    22501 cattcaggat ccgaccgcgg tacggcgcat gtacggcacg ctcctcacga tcgcgatgct
    22561 gaaggaggac tatccctcgg cgaagcagct ggtcgcccag attcgcgacc tcgaggacaa
    22621 gccggcccag aagctcacgt ccgggctcct ggccgaggcg atcatgaagg cgcacgaatc
    22681 gggtgccgcc gacctccacg cggccattcg ttcgaatctc g
//
LOCUS       DASSLZ010000257         3828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_349492, whole genome shotgun sequence.
ACCESSION   DASSLZ010000257 DASSLZ010000000
VERSION     DASSLZ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349492"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFM00_09785"
     CDS             <1..221
                     /locus_tag="VFM00_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498578.1"
                     /translation="YLVKVRKQFFPFAFLDWGAAWFGRENLGRQQPGFDGGAGFLVGE
                     NGLALTVARDLRRSDAPFRFGVRLGASF"
     gene            228..914
                     /locus_tag="VFM00_09790"
     CDS             228..914
                     /locus_tag="VFM00_09790"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HET7498579.1"
                     /translation="MRSRALPFLLALAAIGTTAAAPARADNDLGLAVDTVEVSSGAVL
                     VSYRVENPFTPRLEETLLQGMPATIEYEVGLWKRRVFWFDKLIVAMKNEHRVMYDPWR
                     ERFRVRSGIGIEARTRSFPTIDSLEAVLFDVRRLPVSLLTALEPEGSYYVTVRVLIRP
                     VNEEDLGAIEDWLAGEGKEPEGSHPGLPRYLFGITANLSGLGDRTAVARSVRFTPARR
                     FAAAGVRQEP"
     gene            complement(915..2315)
                     /locus_tag="VFM00_09795"
     CDS             complement(915..2315)
                     /locus_tag="VFM00_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498580.1"
                     /translation="MRAWRSDSGARTASSLRTPRRFLLALLALLIAGLAPAPEALAAP
                     AKTAVTKSAPRPPADTTSDESGFRVRVSETKSGKRAPALPSKPDEVVAPDVPEPPDVP
                     EPPDVNSDEDDNGQNDLVRFGEDITIPADKVVEGNVVAIGGNVTVFGRVKGDVTSVGG
                     TVEVKGAGVVEGDAVSLGGGVNTTDSASVAGSNVSIGNGRFWRGPGMLPMLGLMQPAL
                     FGEWLVGTIVKLALALFFAWIALLLWREGMIRAASVLRDRFGRSFLVGLLTVAGIVVA
                     IPVGIISLVLLAAIAIVILCITIIGIPVAVLLVIALVLAIIGLVVGAIFLMFLGYVNG
                     VHYLGRRVLGERWRDKSPIYAIGVGMLLILALKLMGKIAGFAGIMLFHPLSIAFGIAA
                     GALAFILTVAGLGALWLSFAQGGGLAWRSYNWGPLRRGAPSAEAPPVAPPPGVATPPP
                     PTAEAPRPVDGTSDAP"
     gene            complement(2296..2931)
                     /locus_tag="VFM00_09800"
     CDS             complement(2296..2931)
                     /locus_tag="VFM00_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498581.1"
                     /translation="MKTNLTCAACRSELTALVDGAIHPAVARAILSHVESCAGCRARL
                     EAHRLIAEHARRLPSIEAPAWLEGRVVAAVTRPARVRRLARRAGALALAASFVITAGL
                     LAIYPKLAGQYGLPMPGTAFSRAIGGVIDLLVAVPKQVAAEISFYGPFARQVWAAFHT
                     LGALPRAALVVLQSPESQIAGAFLLTLGVAIFWILRPSRNERGIGHACLAL"
     gene            complement(2928..3509)
                     /locus_tag="VFM00_09805"
     CDS             complement(2928..3509)
                     /locus_tag="VFM00_09805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7498582.1"
                     /translation="MAADERELVRRCLARDDRAYRELVRAYQTPVVNLAWRITGNPDD
                     AAEVAQETFIRVLRSLHTYDPDRPLRSWLFKIASNLALDLIRRRKRRPIALEDLSDED
                     GPAVEAVDPGPGPEEGLRLEWAEERFQALVAELPEHYRAILHLRYKEEFSYEEIAETL
                     GIPLGTVKVRLHRAHENLRRKLTVREPRRGTMP"
     gene            3713..>3828
                     /locus_tag="VFM00_09810"
     CDS             3713..>3828
                     /locus_tag="VFM00_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018451326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498583.1"
                     /translation="MTEIESVFAREILDSRGQPTVEVEVALSGGAIGRAAVPS"
BASE COUNT          578 a         1349 c         1345 g          556 t
ORIGIN      
        1 agtacttggt gaaagtgcgc aagcagttct tcccgttcgc cttcctcgac tggggcgcgg
       61 cgtggttcgg ccgggagaac ctgggccgcc agcagccggg attcgacggc ggcgcgggct
      121 ttctcgtcgg cgagaacggc ctcgccctga ccgtcgcgcg cgacctccgc cgctcggacg
      181 cgccgtttcg cttcggcgtg cggctcggcg cgagcttctg aggcgccatg cgaagccgcg
      241 cgctgccctt cctgctcgcg ttggccgcga tcgggacgac cgccgccgcg ccggcgcgcg
      301 cggataacga cctcggcctc gcggtggaca cggtcgaggt ctcgtccggc gccgtgctcg
      361 tctcctaccg cgtggagaac ccgttcacgc cgcggctgga agagaccctg ctccagggca
      421 tgcccgccac gatcgagtac gaggtcgggc tctggaagcg gcgggtcttc tggttcgaca
      481 agctgatcgt ggcgatgaag aacgagcacc gcgtcatgta cgacccctgg cgcgagcgat
      541 tccgcgtccg ctcggggatc ggcatcgagg cccggacacg ctccttcccc acgatcgact
      601 cgctggaggc ggtgctcttc gacgtccggc gcctccccgt ctcgctcctc accgcgctgg
      661 agccggaggg ctcgtactac gtcaccgtgc gggtgctgat ccggccggtg aacgaggagg
      721 atctgggagc gatcgaggac tggctggcgg gcgaggggaa ggagccggag ggatcgcatc
      781 cgggactgcc gcgctatctc tttggaatca ccgccaacct gagcggcctg ggcgaccgca
      841 ccgcggtggc gcgaagcgtc cgcttcacgc cggcgcggcg ttttgccgcg gccggcgtga
      901 ggcaggaacc ctagctaagg agcgtccgag gtgccgtcca ccggccgggg cgcttcggcc
      961 gtgggcggag ggggcgtcgc gaccccgggc ggcggcgcga ccggaggcgc ctccgcgctg
     1021 ggagcgccgc ggcgcagcgg accccagttg tagctgcgcc aggccagacc gcctccctgc
     1081 gcgaagctga gccacagagc gcccaggccc gccaccgtca ggatgaaggc gagcgcgccg
     1141 gccgcgatcc cgaacgcgat gctgagcgga tggaagagca tgattcccgc gaagcccgcg
     1201 atcttgccca tcagcttgag ggccaggatc aggagcatcc ccaccccgat cgcatagatc
     1261 ggcgatttgt cgcgccaccg ctcgcccagc acgcggcgtc cgaggtagtg gacgccgttc
     1321 acgtagccca ggaacatcag gaagatcgcg cccacgacca gcccgatgat cgcgagcacg
     1381 agcgcgatca cgagcagcac ggcgaccggg attccgatga tcgtgatgca aaggatcacg
     1441 atcgcgatcg ccgcgagcag cacgagggag atgatcccga ccgggatcgc gaccacgatc
     1501 cccgccacgg tgagcaatcc caccaggaac gacctgccga agcggtcccg cagcacgctg
     1561 gcggcccgga tcatgccctc cctccagagg aggagcgcga tccacgcgaa gaagagggcg
     1621 agcgccagct tcacgatggt tccgaccaac cactcgccga acagggcggg ctgcatgagt
     1681 cccagcatcg gcagcattcc gggtccgcgc cagaagcggc cgttcccgat ggagacgttg
     1741 ctccccgcga ccgaggcgct gtcggtggtg ttcacgccgc cgcccaggct gaccgcgtcc
     1801 ccttcgacga cgccggctcc cttcacttcc accgtcccgc cgaccgaggt cacgtctccc
     1861 tttacgcggc cgaagaccgt cacgttgccg ccgatcgcga ccacgttccc ctcgaccacc
     1921 ttgtcggccg ggatcgtgat gtcctcgccg aatcggacca ggtcgttctg gccgttatcg
     1981 tcctcgtcgc tgttgacgtc gggaggctcg ggtacgtccg gcggctccgg aacgtccgga
     2041 gcgaccacct cgtccggctt ggacgggagc gcgggcgccc gtttgccgct cttggtctcg
     2101 ctgacgcgca cgcgaaagcc ggactcgtcc gacgtggtgt cggcgggcgg gcgcggcgcg
     2161 gatttcgtga cggccgtctt ggcgggggcg gcgagcgcct ccggagccgg agccagtccc
     2221 gcgatcaaca gagcgaggag cgcgagcagg aagcggcgcg gagtgcgaag gctcgatgca
     2281 gtccgcgccc cggaatcaga gcgccaggca cgcatggccg ataccccttt cgttgcggga
     2341 cggtcgcagg atccagaaga tggcgacccc cagggtgaga aggaacgcgc cggcaatttg
     2401 cgactccggc gattggagca cgacgagcgc cgcgcgcggc aaggcgccca gcgtatggaa
     2461 cgccgcccag acctggcgcg cgaacggtcc gtagaacgag atctccgccg cgacctgctt
     2521 tggaacggcg acgagcaggt cgatgacgcc gccgatcgcg cgcgagaagg ccgttcccgg
     2581 catcgggagg ccgtactgcc ccgccagctt cggatagatc gcgaggagtc ccgccgtgat
     2641 gacgaaggaa gccgcgagcg ccagggcgcc ggcgcggcgc gcgagccggc ggacgcgcgc
     2701 ggggcgcgtg accgcggcca cgacgcgacc ctcgagccag gccggcgcct cgatcgaggg
     2761 gaggcgccgg gcgtgctcgg cgatcagccg gtgcgcctcg aggcgggcgc ggcatccggc
     2821 gcacgactcg acgtggctca ggatggcccg ggccacggcc ggatgaatgg cgccgtccac
     2881 gagggcggtg agctcgctcc ggcaggcggc gcaggtcagg ttggtcttca cggcatcgtt
     2941 cctcgtcgtg gttctcggac cgtcagtttc cgtcgcaggt tctcgtgcgc ccggtgcagc
     3001 ctcaccttca cggtccccag cggaattccc agcgtttccg cgatctcctc gtaggagaac
     3061 tcctccttgt agcggaggtg gaggatcgcc cggtagtgct cgggcagctc tgccaccagg
     3121 gcttggaacc gctcctcggc ccactccagc cggagccctt cttcgggccc ggggcccgga
     3181 tccaccgcct ccaccgcggg gccgtcctcg tcggagaggt cctccagcgc gatcggccgg
     3241 cgcttccggc gccggatcag atccagcgcc aggttggacg cgatcttgaa gagccaggag
     3301 cggagcggcc ggtccggatc gtacgtatgc agggaccgga gcacccggat gaacgtctcc
     3361 tgcgccacct ccgccgcgtc gtcaggattg ccggtgatgc gccaggccag gttcacgacc
     3421 ggcgtctggt aggctcgcac cagctcgcga taggcccggt cgtcccgggc caggcaccgc
     3481 cggaccagct cccgctcatc cgccgccact agcgccggcc cctcctcggg accgtcacgt
     3541 tcgttacgag tccggagtcc gccaagtttc gtccaaatcg tgtcatcggg gattcgggag
     3601 ggctctccgg cgcatcgccg ggacctgggc gcggaccgta ggcgccccgt ggaaggagcg
     3661 tcaaggcttt tcccttgatc gccgcgcccc gagcgcctag cgttcgggcg ccatgaccga
     3721 gatcgaatcc gtattcgccc gcgaaatcct ggattcccgc ggccagccga ccgtggaagt
     3781 cgaggtcgcc ctctccggcg gcgcgatcgg ccgcgcggcg gtaccctc
//
LOCUS       DASSLZ010000258         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1092830, whole genome shotgun sequence.
ACCESSION   DASSLZ010000258 DASSLZ010000000
VERSION     DASSLZ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092830"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="VFM00_09815"
     CDS             <1..1477
                     /locus_tag="VFM00_09815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7498584.1"
                     /translation="VGLYGALRRRAARAARTIDLKGGTLTPGFTDAHIHLITWRRALD
                     EPWLDAQTPEAIERAVAARLAAAPEREWILVRGWVPREWPLGLRRRDLLDRLAVGRPL
                     LLQAIDGHSVWASAAALERAGITERTPDPSGGRIERDASGGLTGVLIETADRPARAAV
                     VHGEDPARTLRRALARARALGITSAHDFDRSATWRAAQDLEREGALGFRLLLSIPLVA
                     LDDALTLGLRSGFGTDRLRLGAVKMFADGTLGSATALLESPYEGSSDAGMEVTPAAAL
                     ADACRRSVEGGLSVAIHAIGDRAVRHALDAIEVPLSAGLRYPLPPRVEHVQLARREDL
                     ARFRSLGVLASVQPIHQVTDRAVAGAKWGERTGRSYAYRSLLKAGASLLFGSDAPFDR
                     AGPLLGIQAALLRRRAEEPDTMTYHREQRLTLPQALRAHLESPHQAAGWTTPLGRIEA
                     GYGADLVRFDQDLWSVPTDSWHQARVTGVWVGGRLEPIEKM"
     gene            1589..>3478
                     /locus_tag="VFM00_09820"
     CDS             1589..>3478
                     /locus_tag="VFM00_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498585.1"
                     /translation="MKTERLVGLIAGLLLVVVAARPTAAQYYAFGKNKVQYESFQWHT
                     LQGAHVEIYYYPEEEPLARLALVLAEQSYEDLTKKFQHEVDRPVPMIVFASHHHFEQN
                     NVSPFYVPEGVQGFTEYLKGRVVLPYTGSYAEFRHVIHHEMVHVFQFSILDEVYKKHR
                     KSTYLVPPLWFSEGLAEYWSNEWDAQGTMVLSDMVLEGQLPTINDLWRYNGTFTIYKL
                     GQSICQYIGETYGQDALRRLYTDAYKDDRFEKLIGRVTGVPANKVSEDWIYAMKRRYY
                     PEVKDHVSLTSAAEERAVSQGVNLKPVAVPDTTILGGYRYLFMSARSGYTNIYSASWK
                     GRERDVKSVVRGQRTAQFESFHPFQSRIDANRKGELAFVSKWNESDALFVMDLATKKV
                     TFKKHFDGMTTLASPAWSPDGTAIAFAAITPAGQSDLYLFHPATGELRRLTNDDYADA
                     DPTWSPDGKTIAFASDRTRYGKDGHQNLFLLDVASGEVRYLTCGPWIDQSPRWSPDGK
                     RIAFASDRDGGFNLYAVDPQGNGARLTKLLGTALDPAWTPDGRSLLFTGFRKQGFGIY
                     QLPVQPDEIPAATAATATAHGSSAGTSASLAPGALAGADPARPASPGTSVSAPADPKP
                     ADPKPE"
BASE COUNT          550 a         1217 c         1163 g          548 t
ORIGIN      
        1 ggtcggcctc tatggggcgc tgcgccgccg ggcggcgcgc gcggcacgga cgatcgatct
       61 gaagggcggc accctgacgc ccggattcac ggacgcccac atccacctga tcacctggcg
      121 gcgcgcgctg gacgagccgt ggctggacgc gcagacgccc gaggcgatcg agcgcgccgt
      181 ggccgcgcgc ctggcggcgg cgcccgagcg ggaatggatc ctggtgcgcg ggtgggtgcc
      241 tcgcgaatgg cccctggggc tccgccggag agacctcctc gaccggctcg ccgtgggccg
      301 tccgctcctg cttcaggcga tcgacgggca ctcggtctgg gcgagcgcgg ccgcgctgga
      361 gcgggcgggg atcaccgagc gcacgcccga tccctccggc ggccggatcg agcgtgacgc
      421 ctcaggcggg ctcaccggcg tcctgatcga gacggcggat cggcccgcgc gagccgcggt
      481 cgtgcacggc gaggacccgg ccaggaccct gcgtcgggcg ctggcgcgcg cccgcgccct
      541 gggaatcacg agtgcgcacg acttcgaccg ttccgccacg tggcgcgcgg cgcaggacct
      601 ggagcgggaa ggggccctgg gattccgact cctcctctcg attccactcg tcgctctgga
      661 cgacgcgctg accctggggc ttcggagtgg attcggaacc gatcgtctcc gtctcggcgc
      721 ggtgaagatg ttcgcggacg gaacgctcgg gtcggcgacc gcgctgctcg agtcgcccta
      781 cgagggatcg tccgatgcgg gaatggaggt caccccggcc gccgcgctcg cggacgcctg
      841 ccggcggtcc gtcgaggggg gcttgtccgt ggcgatccac gcgatcggag atcgcgcggt
      901 ccgccacgcg ctggacgcga tcgaggtccc gctgagcgct gggcttcgct acccgctgcc
      961 gccccgtgtg gagcacgtgc agcttgcgcg aagggaagac cttgcccggt tccgttcgct
     1021 tggcgtctta gcctcggtcc agcccatcca ccaggtcacc gaccgagcgg tggccggggc
     1081 gaaatggggg gagcggaccg gccgctcgta cgcctaccgc tcgctcctca aggcgggcgc
     1141 ttcgctactc ttcggatcgg acgccccgtt cgaccgggcc ggtccgctgc tcggcatcca
     1201 ggccgccctg cttcgaagac gcgcggagga gcccgacacc atgacgtatc atcgagagca
     1261 gcgcctgacg ctgccccagg cgctccgcgc ccacctcgag tcgccgcacc aagccgccgg
     1321 gtggacgacg ccgctcggga ggatcgaagc gggctacgga gccgatctgg tccgtttcga
     1381 ccaggatttg tggtccgttc cgaccgattc ctggcaccag gcgagggtca ccggtgtttg
     1441 ggtgggcgga aggctggaac ctatcgaaaa aatgtagtct tagacgtctc tcttcttgtc
     1501 ccggtacatc gtgcgctggt agaatgtttc ttgcgtctcc caccagtcgt cgtctcgccg
     1561 ggcggactca tccgaaggcc gctcttgaat gaaaactgaa cgtctcgtcg ggctcatcgc
     1621 agggctgctt ctcgtcgttg tcgccgcacg tcccactgcc gcgcagtact acgcctttgg
     1681 caagaacaag gtgcagtacg aatcattcca gtggcacacg ctccagggcg cccacgtcga
     1741 gatctattac tacccggagg aagagcccct cgcgcggctc gctctggtgc tggccgagca
     1801 gagctacgag gacctgacga agaagttcca gcacgaggtc gaccggcccg tgccgatgat
     1861 cgttttcgcc tcccaccacc acttcgaaca gaacaacgta agtcctttct acgtacctga
     1921 aggcgtccag ggcttcacgg aatacctgaa gggtcgcgtg gtcctgccct acaccggctc
     1981 gtacgccgaa ttccggcacg tcatccacca cgagatggtg cacgtcttcc agttctccat
     2041 cctcgacgag gtctacaaga agcaccggaa gagcacctac ctcgtgccgc ccctctggtt
     2101 ctccgagggg ctggccgagt actggtcgaa cgagtgggac gcgcagggga cgatggtcct
     2161 ctccgacatg gtcctcgaag ggcagctgcc gacgatcaac gacctctggc gctacaacgg
     2221 gacgttcacc atctacaagc tggggcagtc gatctgccag tacatcgggg agacgtacgg
     2281 acaggacgcg ctgcgacgcc tgtacaccga cgcctacaag gacgaccgct tcgagaagct
     2341 gatcggccgg gtcacgggcg ttccggcgaa caaggtctcg gaagactgga tctacgcgat
     2401 gaagcggcgc tactaccccg aggtgaagga tcacgtctcg ctcacctcgg ccgccgagga
     2461 gcgcgccgtc tcgcagggtg tcaatctcaa gccggtggcg gtgcccgaca ccaccatcct
     2521 gggcggctac cgctacctct tcatgtccgc ccggagcggg tacacgaaca tctactccgc
     2581 ctcgtggaag gggcgcgagc gcgacgtgaa gtcggtggtg cgcggccagc gcacggcgca
     2641 gttcgagtcg ttccacccct tccagagccg catcgacgcc aaccggaagg gtgagctggc
     2701 gttcgtctcc aagtggaacg agagcgacgc cctcttcgtg atggacctcg ccaccaagaa
     2761 ggtgacgttc aagaagcact tcgacgggat gacgacgctg gcctcgcccg cgtggtcgcc
     2821 cgacgggacc gcgatcgcgt tcgccgcgat cacgcccgcg ggacaatccg atctctatct
     2881 cttccatccc gccacgggcg agctgcggcg gctgaccaac gacgattacg ccgacgccga
     2941 ccccacctgg tcgcccgacg ggaagacgat cgcgttcgcc tcggaccgca cgcgctacgg
     3001 caaggacggg caccagaacc tgttcctcct cgacgtggcg agcggcgagg tccgctatct
     3061 gacgtgcggc ccctggatcg atcagtcgcc gcggtggtcg cccgacggga agcggatcgc
     3121 gttcgcgtcc gaccgggacg gcggcttcaa cctgtacgcc gtggatccgc agggcaacgg
     3181 cgcgcgtttg acgaagcttc tcggcaccgc gctcgacccg gcgtggacgc ccgacggccg
     3241 ctcgctcctc ttcaccgggt tccggaagca gggattcggc atctaccagc tcccggtcca
     3301 gccggacgag attccggccg cgaccgccgc caccgcgacg gcgcacgggt cctccgcggg
     3361 gacgtcggcg agcctcgctc cgggcgccct tgcgggcgcc gatcccgccc gcccggcgtc
     3421 tcccggcacg agcgtatcgg ctccggccga tccgaagcct gccgacccca agcccgag
//
LOCUS       DASSLZ010000259         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1721257, whole genome shotgun sequence.
ACCESSION   DASSLZ010000259 DASSLZ010000000
VERSION     DASSLZ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721257"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VFM00_09825"
     CDS             <1..768
                     /locus_tag="VFM00_09825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7498586.1"
                     /translation="SVPSVSIVILTRNRPAYLPRAIRSAGAQTHAELELVLVRDGGEP
                     LDPESRAAIDALDFPALLVERDGPAEGVARSRNEGIGSARADAVAILDDDDTWDPPHV
                     AHLAALFDRDPSVDVAYSDARIRLEDGGGERVIARDFDLAVFSRDDYAPPSTMLIRRA
                     AFERFGVFDPDFTCSEDWEWMLRVARRGGVITRSPGVTATILIHKGAHSALQLARLEE
                     RKRCLELLSKRHDLPPLTPKTYWEVAETVCPGSGTAR"
     gene            741..1718
                     /locus_tag="VFM00_09830"
     CDS             741..1718
                     /locus_tag="VFM00_09830"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7498587.1"
                     /translation="MPRKRHGALTDVLIVGAGPAGLSAALWLRDFGLSCLLLERERQP
                     GGQLHQIHAPIPNYLTAYGWDGARFAGVALDDARAASLEILVGEPVRRIVVERPTRSG
                     PRRGGPVLRVDRGRERLRGRALILATGLRRRALGVPGEKELAAHGVSHSANRDRMRWA
                     QRPVAVIGGGTAAVEDAILCAEVGCDVLLLHHSARFRARNDFLERARAHPKIRIVTNA
                     EVRAIVGKERVEAVRFREQGKRRDEEASVDAVYVRIGWEPATEAARGVVRLDRAGYVR
                     VGPSGRTSARGIYAAGDVCSPSCPSIAGAVGQGAAVAWEIARALSRLPR"
     gene            complement(1726..>2039)
                     /locus_tag="VFM00_09835"
     CDS             complement(1726..>2039)
                     /locus_tag="VFM00_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498588.1"
                     /translation="RWLGHQTSLEFSPGVVVTGDGPFQGPGFVGQAAFNAGDLVSLVF
                     EGEVGRFSWPDSWNRPAREVTGSSLRVGIRGGSYVGAAAGVILGMVVGAASIPDWGSG
                     W"
BASE COUNT          288 a          761 c          717 g          273 t
ORIGIN      
        1 tccgtgccct ccgtctcgat cgtcatcctg acccgaaatc ggcccgcgta cctcccgcgc
       61 gcgatccgtt cggcgggcgc gcagacccat gccgagctgg agctggtgct ggtccgggac
      121 ggcggcgagc cgctcgatcc cgagtcgcgc gccgccatcg acgcgctgga cttccccgcg
      181 ctcctggtcg agcgggacgg tccggcggaa ggcgtggccc gctcgcggaa cgaggggatc
      241 gggagcgcgc gcgccgacgc ggtcgcgatc ctggacgatg acgacacctg ggacccgccg
      301 cacgtggcgc acctcgccgc gctcttcgat cgcgatccca gcgtggacgt ggcctactcc
      361 gacgcgcgca tccgcctcga ggacggcggc ggcgagcggg tgatcgcccg cgatttcgac
      421 ctcgccgtct tctcgcgaga cgattacgcg ccgcccagca cgatgctgat ccgccgcgcg
      481 gccttcgagc gcttcggcgt gttcgacccc gacttcacct gctccgagga ctgggagtgg
      541 atgctgcgcg tcgcccgccg tggcggcgtc atcacccgct ctcccggcgt gaccgcgacg
      601 atcctgatcc acaagggagc gcacagcgcc cttcagctcg cgcgcctcga ggagcggaag
      661 cgctgcctcg agctcttgag caaacggcac gacctgccgc cgctcacccc gaagacctac
      721 tgggaagtgg ccgagaccgt atgccccgga agcggcacgg cgcgctgacc gacgtcctga
      781 tcgtgggcgc cgggcccgcc ggactctcgg ccgcgctatg gctccgcgat ttcgggctct
      841 cctgcctgct cctcgagcgg gagcggcagc ccggtggcca gctccaccag atccacgccc
      901 ccattcccaa ctacctcacc gcctacggct gggacggcgc gcgcttcgcc ggcgtggcgc
      961 tggacgacgc gcgcgccgcg tcgctggaga tcctcgtggg cgagccagtg cggcggatcg
     1021 tcgtggaacg ccccacgcgg agcggcccac gccgcggggg gcccgtgctt cgcgtggacc
     1081 ggggccgcga gcgcctgcgg gggcgagccc tgatcctcgc gaccggtctg cgccgccgcg
     1141 cgctcggcgt gcccggcgag aaggagctgg ccgcgcacgg cgtcagccac tccgccaacc
     1201 gcgaccggat gcgctgggcc cagcgccccg tcgccgtgat cggcgggggc acggccgccg
     1261 tggaggatgc gatcctgtgc gcggaggtcg gctgcgacgt cctgctgctc caccactcgg
     1321 cccggttccg ggctcgcaat gactttctgg agcgcgcgcg ggcccatccg aagatccgga
     1381 tcgtgacgaa cgccgaagtg cgcgcgatcg tcgggaagga gcgcgtcgag gcggtgcggt
     1441 ttcgggagca aggcaagcgg cgcgacgaag aggcgagcgt ggacgccgtc tacgtgcgga
     1501 tcggctggga gcccgcgacg gaggccgccc gcggggtcgt ccggctggac cgcgccggct
     1561 acgtgcgcgt cggacccagc ggtcgaacga gtgcgagagg gatctacgcc gccggagacg
     1621 tctgctcgcc gagctgtccg tcgatcgccg gcgcggtggg ccagggcgct gccgtagcct
     1681 gggagatcgc ccgggcgtta agccggctgc ctcggtaagc gctccttacc agccgcttcc
     1741 ccaatcgggg atcgacgccg cgcccacgac catcccgagg atgactcccg cggcggcccc
     1801 aacgtacgag ccaccccgaa tcccgacccg aagggagcta ccggtcactt cgcgagccgg
     1861 tcgattccag gaatctggcc aggagaagcg gcccacctcc ccctcgaaga ccaagctcac
     1921 caggtcgccc gcattgaacg cagcctgtcc cacgaacccc ggcccctgaa acggaccatc
     1981 tccggtcaca acgaccccgg gggagaactc gagggatgtc tggtgcccga gccaacggc
//
LOCUS       DASSLZ010000260         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1488509, whole genome shotgun sequence.
ACCESSION   DASSLZ010000260 DASSLZ010000000
VERSION     DASSLZ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488509"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFM00_09840"
     CDS             complement(<1..289)
                     /locus_tag="VFM00_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HET7498589.1"
                     /translation="MTRRLYYEDSRRDQFEAVVLSARPDEGGTRVVLDATAFYPGGGG
                     QPFDRGVLGGRAVLEVGLEGDELWHRVLGALESGARVEGRLDWARRFDHMQQ"
     gene            complement(410..907)
                     /locus_tag="VFM00_09845"
     CDS             complement(410..907)
                     /locus_tag="VFM00_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498590.1"
                     /translation="MPRTRQKLATLDAVEEIARRVPGYKGYQEMSQRREDDRRFRSSI
                     AEHLAGEAHRLERIESHQFRDDFSDLLEEVDAGARKLEFLADAIAMPSPAQKGTFSDE
                     AAEGAGTIDRQILDELDRLHRLVHELEKAYVHDERFEMNLAELRGLTESIADLIEQRN
                     VALTR"
     gene            complement(1123..2418)
                     /locus_tag="VFM00_09850"
     CDS             complement(1123..2418)
                     /locus_tag="VFM00_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498591.1"
                     /translation="MSRSRIPRAALAFALLAAAALGAGGGCALRDRSNPLDPKNRGVT
                     HGLLGGFNAVAGDNVVLLRWTPVALAGVESYRVDRWHPGEAPRPLSGALYPPYTSSAE
                     DLSARNDSVYVYRVVARFGSGDSVASPPDTATPGTRRIMVLVADLPGVAGLSPDMRDI
                     VYGDVASEPYDEIDLDSTRGVFWLSQYERGVIASRRFETLNGGVEFQSYHPTDIAITT
                     QRPTIWAAQPELSQVSRLLAGDTTGSAIAGVGPARVVESNSANATLWIGADDGGLYHA
                     SAATLDTLRSWHFSGRVGPIAVDVASDEAWVAVRASDLFDLYLVAAGNPVPLRLRTGL
                     LNVTDIKVEGATRTAWVSERGAPLAGNGRLSRVDRDGTVLATLVGIEPYALSVEPGSS
                     EVWVTDIRSDRLIEVSPAGAIVRRSPRMGVPYGVRVYRP"
     gene            complement(2415..3116)
                     /locus_tag="VFM00_09855"
     CDS             complement(2415..3116)
                     /locus_tag="VFM00_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498592.1"
                     /translation="MLVDGVRVGRSPVGPLWVPAKAVLVQALSDDPRRFEPGRDTQLV
                     TPRAGDALVVTIDLRPSVLLRTEPEPADLFLREAAPGGDSLLGSTPLRLLPSRLEGRD
                     FRIAAPEHADTLLAGDALLALAPSGGPASVRMRRVAPHILPAAPKAPPLYRRRWFQLA
                     MIGAGAALTGTSAALRHQADQWYDRYLESSDSRAIPGLYDRTVHYDRLAGGTLASGQV
                     LLTAGIFLLVTSGRR"
BASE COUNT          529 a         1209 c         1262 g          476 t
ORIGIN      
        1 gctgctgcat gtggtcgaag cggcgcgccc agtccaggcg tccttcgacg cgcgcgcccg
       61 attccagcgc gcccaggacc cgatgccaga gctcgtcccc ttcgagcccg acctccagga
      121 cggcacggcc gcccagcacg ccacggtcga aaggctggcc gccgcccccg gggtagaagg
      181 cggtcgcgtc caggaccacg cgcgttcctc cctcgtcggg acgcgcgctc aggacgacgg
      241 cttcgaactg gtcgcggcgg gaatcttcgt aatagaggcg acgggtcaag aaggagcgtt
      301 cctcggaccg ggcgatggga gacggtggtt ctacaataca aggaggctcc ccgtacggca
      361 atcggggagc ctcgaatcgc gttaagggcg gcggccgcgc cgcgggaagc tagcgggtca
      421 gggctacgtt ccgctgctcg atcaggtcgg cgatcgactc cgtgagtccg cggagctccg
      481 ccaggttcat ctcgaagcgc tcgtcgtgga cgtaggcctt ttccagctcg tgcaccagcc
      541 ggtggaggcg gtccagctcg tccaggatct gacggtcgat cgtgcccgcg ccttccgcgg
      601 cctcgtcgga gaaggtcccc ttctgcgcgg gcgagggcat cgcgatggcg tcggccagga
      661 actccagctt gcgagcgccg gcgtccacct cttccagcag gtcggagaag tcgtcacgga
      721 actgatggct ctcgatccgc tccagccggt gcgcttcacc ggcaaggtgc tcggcgatgc
      781 tgctccggaa tcgccggtcg tcctcgcggc gctgcgacat ctcctggtat cccttgtagc
      841 cggggacccg ccgcgcgatc tcttcgacgg cgtccagcgt ggcgagcttc tggcgggtgc
      901 gtggcattct cctcatcctc ctgaatgatg cagagtgcac gattcgccac cagtgcatgg
      961 ccggaagcga catcgagcga cggctcagat cccgtactcg cggcgcgtgc cgtcatcgct
     1021 atccggttga gcgaccggta gatgcttccc atttccgagg ccggcccgat cccttccgcg
     1081 ccggcgtcgt gggagccacg ctgcaaccgg tgtgccacgg cgttacgggc ggtagacgcg
     1141 cacgccgtag ggcacgccca tccgtggcga ccgccgcacg atcgctccgg ccggggacac
     1201 ttcgatcagg cggtccgatc ggatgtcggt cacccagacc tcgctcgatc cgggctccac
     1261 cgagagcgcg tagggctcga tgccgacgag cgtcgcgagc accgtgccat cgcgatcgac
     1321 gcgtgagagc cgtccgtttc cggcgagcgg cgcgccccgc tcgctgaccc acgcggttcg
     1381 ggtggccccc tccactttga tgtcggtcac gttcagcaga cccgtgcgca gtcgcagcgg
     1441 cacgggattc ccggcggcga cgaggtagag atcgaagaga tcgctcgcgc ggaccgcgac
     1501 ccacgcctcg tccgaggcga cgtccaccgc gatcggtccc acccgcccgg agaaatgcca
     1561 ggatcgcagc gtgtcgagcg tggctgcgct cgcatggtag aggccgccgt cgtcggcgcc
     1621 gatccagagc gtcgcgttcg cgctgttcga ctccacgacg cgggccggcc ccaccccggc
     1681 gatcgcgctc cccgtggtgt cccccgcgag caggcgggag acctgggaca gctcgggctg
     1741 ggcggcccag atcgtgggac gctgcgtcgt gatcgcgatg tcggtcggat ggtaggactg
     1801 aaactcgacg ccgccgttca gcgtctcgaa gcggcggctg gcgatgacgc cccgctcgta
     1861 ctggctgagc cagaagacgc cccgcgtgga gtcgagatcg atctcgtcgt agggctcgga
     1921 ggcgacgtcg ccgtagacga tgtcgcgcat gtccggcgag agccccgcca cgccggggag
     1981 atcggccacg aggaccatga tgcggcgcgt gcccggcgtc gcggtgtcgg gcgggctcgc
     2041 gacgctgtcc cccgagccga accgggcgac gacccgatag acatagacgg agtcgttccg
     2101 ggccgagagg tcttccgccg agctggtgta gggcggatag agcgctcccg aaagcggccg
     2161 gggcgcctcg ccggggtgcc aacggtcgac gcgatagctc tcgacgccgg ccagcgccac
     2221 cggcgtccag cggaggagga ccacgttgtc ccccgccacc gcgttgaagc cgccgagcag
     2281 gccgtgcgtg acgccccggt tcttcgggtc gagcgggttg gagcggtccc gcagcgcgca
     2341 tccgccgccg gcgccgagcg ccgcggcggc gaggagcgcg aacgcgagcg ccgcgcgcgg
     2401 gatgcgggaa cggctcatcg ccggccgctc gtcacgagca ggaagatccc cgcggtgagc
     2461 agcacctgac cggacgccag cgtccctccc gcgagacgat cgtagtgcac ggtccggtcg
     2521 tagaggcccg ggatcgcgcg cgagtcggac gattcgaggt agcggtcgta ccactggtcc
     2581 gcctgatgcc ggagcgcggc cgacgtgccg gtcaacgccg cccccgcccc gatcatcgcg
     2641 agctggaacc accgccgccg gtagaggggc ggggccttgg gcgccgcggg aagaatatgg
     2701 ggggcgaccc gcctcatgcg gacgctcgcg ggcccgccgg agggagccag ggcgagcagc
     2761 gcgtcgccgg cgaggagggt gtcggcgtgc tcgggcgcgg cgatccggaa atcacgcccc
     2821 tcgaggcgcg acggcagcag gcggagcggc gtggatccca ggagcgagtc cccgccgggc
     2881 gcggcctcgc gcaggaagag gtccgccggc tcgggttcgg tgcgcagcag caccgagggg
     2941 cggagatcga tcgtgacgac gagggcgtcc ccggcccggg gcgtcacgag ctgggtatcg
     3001 cggcccggct cgaagcggcg cggatcgtcg gagagggcct ggacgagaac cgccttggcc
     3061 ggcacccaga ggggccccac gggggagcgc cccacccgca cgccgtccac gagcacgacg
     3121 agacccgcgg gcttcgtcgt gacgcggagc gacgcgaccg cggaatcggg gcgtgcgctc
     3181 tcgggaacca gggtggaccc ggcgcgcgcg ctccccgccg cggcaagcgc gacggggagc
     3241 gtcaggaaga cgagagcgag ccggagccgg gacgcccttc cctgggccgc gcgcgggtta
     3301 gaagtcgaac ccgagataga actccttctc ccaacccgac tggacgctct tgaaatcggt
     3361 gcggcgggcg aggtcatacc gaagaaccat atacccgccc aggctcatgc gaaggctcaa
     3421 acccatggag cccttcggac gcgggaacga ctcgaacttc tcccacgcgt tccccg
//
LOCUS       DASSLZ010000261         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1235308, whole genome shotgun sequence.
ACCESSION   DASSLZ010000261 DASSLZ010000000
VERSION     DASSLZ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235308"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFM00_09860"
     CDS             complement(<1..334)
                     /locus_tag="VFM00_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498593.1"
                     /translation="MIPEKRFWLLRRLAKAMTIGAWLVFALIVVVTPVGIARSVMSGD
                     KLELIEWLKYGASGGLIFVNLLWIAQSIQVILAIEENTKQSTYVLEKLTTLMQQVRDR
                     VGEVVERDE"
     gene            complement(340..756)
                     /locus_tag="VFM00_09865"
     CDS             complement(340..756)
                     /locus_tag="VFM00_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498594.1"
                     /translation="MLDGYRAACYPSPVATRPPYEAISEREIATGRALAAVAYLPGLF
                     LIGLLDAPRNRFVRFHARQGLVLFLAEAAALVAIAIVDGSLGRIPVLGVVVGAVLRLA
                     LGLLFLAVALYGIAKALSGETVRIPYLGDVADRMEV"
     gene            complement(749..>2603)
                     /locus_tag="VFM00_09870"
     CDS             complement(749..>2603)
                     /locus_tag="VFM00_09870"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HET7498595.1"
                     /translation="APAALTPEDRAALLRDFFSVHRERMLEPRPRYRDLLRRAGEERS
                     GGAPLTEAEVRDLQVWFHLAWTDPSYAAEEPLRSLLAKGEGYTEAEKQALLDWGRALA
                     GTVIPAYREASTAGQVEIAASAHYHPILPLLADSDAPREAGAAFPLPDPPFRAPEEAE
                     AQLKSARASHARRFGAPPRGTWPPEGAVNDAALGLIRAQGFTWAASDETVLRRALAKE
                     DGIDPPDWPAARCRPYRVETAAGPLAMVFRDRALSDRIGFVYQRWEAVDAAEDFIEQI
                     RSSAAHAKGSGPALVTVILDGENCWEGYEEDGRPFLEALYERLEAHPSIEAVTVSQAL
                     ERMPPTETLAHVPVGSWIRDDLAIWIGHPEKNAAWHALRDARALVAARGADVPADVHE
                     ALQAAEGSDWFWWYGDDHESDHKDVFDALFRGLLLGVYASLGAEPPATLRQSLRLAAG
                     VEEAGEHGPEWAGAARYEEDGPEWAGAARYEAAGASGSMHRVSGLLERIRWRMEGNQL
                     LVRIRLVPGASRDGAVLRLLAGEARSARIPLDGADRGGLAWDDASSGGSYERGEAVDV
                     RIPLALLPEGTRSWRLEVGRGEAAEERAPHEGWFDAAGPGGATGEAGTPHA"
BASE COUNT          392 a          988 c          874 g          349 t
ORIGIN      
        1 cctcgtcccg ctctacgacc tcaccgaccc gatcccgcac ctgctgcatc agcgtcgtca
       61 gcttttcgag aacgtacgtc gactgcttgg tattctcctc gatcgccaag atcacctgga
      121 tcgattgcgc gatccagagc aggttcacga agatcagccc gcccgaggcg ccgtacttga
      181 gccattcgat cagctccagc ttgtcgccgc tcatgacgct ccgggcgatc ccgaccggcg
      241 tgaccaccac gatcagcgcg aacacgagcc aggcgccgat cgtcatcgcc ttggccagcc
      301 ggcggagcag ccagaagcgc ttctcgggga tcatcgacat caaacctcca tgcggtcggc
      361 cacgtcgccg agatatggga tccggaccgt ctcgcccgac aacgccttcg cgatgccgta
      421 cagggccacg gccaggaaca acagtcccag ggccagccgc agcacggccc ccacgaccac
      481 gcccagcacc gggatgcgcc cgagggatcc gtccacgatg gcgatcgcga cgagcgccgc
      541 cgcttccgcc aggaagagca ccagcccctg gcgcgcgtgg aagcgcacga agcggttccg
      601 cggcgcgtcg aggagtccga tcaggaagag tccggggaga taggcgaccg cggcgagcgc
      661 ccggccggtc gcgatctcgc gctcggatat cgcttcgtac ggcggccgtg tcgccacggg
      721 ggacggatag cacgcggccc ggtagccgtc aagcatgggg cgtcccggct tctccggtcg
      781 ctccgcccgg acccgccgcg tcgaaccatc cctcgtgggg cgcgcgctcc tccgcggctt
      841 ccccgcgtcc gacctccaga cgccacgagc gcgttccctc cggcaagagc gcgagcggaa
      901 tccggacgtc caccgcttcc ccgcgctcgt aggagccgcc ggaggaggcg tcgtcccacg
      961 cgagcccgcc acgatcggcg ccatccaggg gtatccgcgc cgaccgcgcc tctccggcaa
     1021 gcagcctcag cacggcgccg tcccgcgagg cgcccggaac cagtcggatc cgcacgagca
     1081 gctgattgcc ttccatgcgc caccggatcc gctcgagaag acccgagaca cggtgcatcg
     1141 agccggatgc gcccgcggcc tcgtagcgcg ccgcgccggc ccactccggc ccgtcctcct
     1201 cgtagcgcgc cgcgccggcc cactccggcc cgtgctcgcc ggcctcctcc acgcccgcgg
     1261 ccaggcggag actctgacgc aaggtcgccg gaggctcggc gcccagggac gcgtacacgc
     1321 cgagcagaag cccccggaac agcgcgtcga agacgtcctt gtgatcgctc tcgtggtcgt
     1381 cgccgtacca ccagaaccag tcgctcccct ccgccgcttg cagcgcctcg tgaacgtcgg
     1441 ccggcacgtc cgcgccgcgc gcggcgacga gcgctcgcgc gtcgcgcagc gcgtgccagg
     1501 cggcgttctt ttcgggatgc ccgatccaga tcgcgaggtc gtcccggatc caggatccca
     1561 cgggtacgtg cgccaatgtc tcggtcggcg gcatccgctc caaagcctgc gagaccgtca
     1621 cggcctcgat cgagggatga gcctcgaggc gctcgtagag cgcttcgaga aagggacgcc
     1681 catcctcctc gtaaccctcc cagcagttct cgccgtccag gatcaccgtc acgagcgcag
     1741 gtcccgatcc cttcgcgtgc gccgccgagg agcggatctg ctcgatgaaa tcctccgccg
     1801 cgtccacggc ctcccagcgt tgatagacga acccgatccg gtcggagagc gcgcggtcgc
     1861 gaaagaccat cgcgaggggg ccggccgccg tctcgacgcg atagggccgg cagcgcgcgg
     1921 cgggccagtc gggcggatcg atgccgtcct ccttcgccag cgcgcggcgg agcaccgttt
     1981 cgtccgaggc ggcccaggtg aacccctgcg cgcggatcaa cccgagcgcc gcgtcgttca
     2041 ccgcgccctc cgggggccag gtgccgcgcg gcggagcgcc gaagcgccgc gcatggctcg
     2101 ctcgggcgct cttcagctgc gcctcggctt cttccggcgc gcgaaacggc ggatcgggaa
     2161 gcggaaacgc ggcgcccgct tcgcgcggcg cgtcgctgtc ggcgaggagc ggcaggatcg
     2221 gatggtagtg ggcggaggcg gcgatctcga cctgccccgc cgtggacgcc tcgcggtagg
     2281 ccgggatcac cgtccccgcg agcgcgcggc cccagtcgag gagcgcctgc ttctccgctt
     2341 ccgtataccc ctcccctttc gcgaggaggg aacggagcgg ctcttcggcg gcgtaggagg
     2401 ggtcggtcca ggccaggtgg aaccagactt gcaagtcgcg cacctcggcc tcggtcagcg
     2461 gcgcgccgcc ggagcgctcc tcccccgcgc ggcggagaag gtcacgatag cgcggccgcg
     2521 gctcgagcat ccgctcgcga tgcaccgaga agaagtcgcg gagcagcgcc gcgcggtctt
     2581 cgggggtgag cgcggcgggc gcc
//
LOCUS       DASSLZ010000262         9782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_603509, whole genome shotgun sequence.
ACCESSION   DASSLZ010000262 DASSLZ010000000
VERSION     DASSLZ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603509"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="VFM00_09875"
     CDS             complement(<1..1519)
                     /locus_tag="VFM00_09875"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7498596.1"
                     /translation="MPRQTMHLTATGGATAAPPAKPAADPGPAREGDVVLTVHGMTCA
                     GCVATVERALRGEAGVASAEVNLATEEARVRVVPGTKPSVAALVSAVERAGYRAAPRA
                     STSSSEREKVRLAEERALRGRLLLSVALGVPIMVVHLFVHDQDRARWLALLLATPVQW
                     IAAWPFHARAVKALRHRTADMNTLITLGTFSAYLYSAAVTLAPGRFRASGLGTHVYFD
                     TAVMILVFILLGRLLESRARRRTGDALKALLTRRPPTARRLTAEAAGGEPGFDAPRES
                     VPVESVARGDLLEILPGEVIPVDGLIAEGRTEVDESMLTGEPFPVAREPGDPVAAGTV
                     NRIAPFILRATRVGAETTLARIARLVEEAQGGKAPMQELADRVAARFVPAVIAIAIAA
                     AAIWLFVGPEPRARYALTVFVSVLIVACPCAMGLATPTAILAGTGAGARRGILIRGGE
                     ALERAGSLTTVVFDKTGTLTRGEPVVESLAAAPGIDPARMLAAAAAVEAGSEHPLA"
     gene            1645..2727
                     /locus_tag="VFM00_09880"
     CDS             1645..2727
                     /locus_tag="VFM00_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET7498597.1"
                     /translation="MAQAPVSEAQVLDALRSVMDPDLHRDIVSLGFIRNMKIEGGTVA
                     FDVNLTTPACPVKDRLRDQARQAVLAIPGVSDARVNMTAEVRRPQAPSTAALQNVKNI
                     VAVGSGKGGVGKSTVAANLAASLARSGARVGLLDADIYGPSIPIMMRGSRPAEPRADN
                     TIEPGDAHGTKVMSMGYLTPGDQPLIWRGPMAHKAVQQCLLGVNWGELDYLIVDLPPG
                     TGDVHLTLVQTVPVTGGVIVSTPQDVGLQISMKTLRMFQQTKVPILGIVENMSYYVCP
                     HCGGRDDLFGHGGAEEAAKALDVPFLGSIPLDPAIRRAADEGTPVVVAEPESRSGKAM
                     ADVAGALARQVSIRAFRNLTLSIVEE"
     gene            2727..3068
                     /locus_tag="VFM00_09885"
     CDS             2727..3068
                     /locus_tag="VFM00_09885"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET7498598.1"
                     /translation="MARVLLPEETTPVALKKKGGERFTIRWEDGHESVYSARTLRGRC
                     PCAQCVSETTGERMVFEEHVDPGVSIAAARTVGNYALHFDWSDGHTTGIYAFDYLRRI
                     CPCAACTGQAG"
     gene            3144..4346
                     /locus_tag="VFM00_09890"
     CDS             3144..4346
                     /locus_tag="VFM00_09890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7498599.1"
                     /translation="MWTAQFFTMLGMSMFLPFLPLYLRQLGVTEPGKLEFVSGLLFAA
                     PFFAATVATPVWGVLGDKHGRKLMVVRASLGLSLATLLMGFARTVPQLFVLRVAQGAV
                     SGFIAAALALMASKAPRERMGYALGTLQTAIPTGTILGPLIGGALADAIGYRQIFFVT
                     AAMNLTAGIAVILLVPRDLPEPREAGFGQVFENYRTVFGTPQFRLLFFVLFGSQFALM
                     SIQPVMALYVEYLGTSGRLLATTTGVIFAITGVASAIGAPRWGRRGDRKGYRPVLGRA
                     LLGAALFALPQALVARAWQLFLLRIGYGAFIGGIVPSVQAMIGIRAPAERRAGIMGVT
                     STALMMGNLTGPLIGGAVAGSFGLRSVFVLATAVFVALLAALYPRMAEPARPETVRPE
                     PAAQVVTV"
     gene            complement(4372..4752)
                     /locus_tag="VFM00_09895"
     CDS             complement(4372..4752)
                     /locus_tag="VFM00_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7498600.1"
                     /translation="MLRESRAFSGFSVDDIPKAKAFYGETLGLDVREENGMLHLHLPG
                     GAVVLAYPKPNHQPATFTILNFPVASVEEMVDELTARGVPFEQYGGEIQTDAKGIHRG
                     KGPVIAWFKDPAGNILSILETTKR"
     gene            4969..5952
                     /locus_tag="VFM00_09900"
     CDS             4969..5952
                     /locus_tag="VFM00_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498601.1"
                     /translation="MQFRTTLAGSYPKLPTRSGDVNLRVTLNRRDQGKATDQDVADAI
                     RATTRRTLAMEEQAGLDLLMDGGAGWEDAQTYVARGLEGMRIAGLIRYLDTNTYYRQP
                     EIVGPVAWKGPITVGDYLAATAMTKKPVKAFLPGPYSLYRFSKDLHYGDAVAAGAAIG
                     KALAREAAALEEAGASWIHFEEPWLGRARAEDASAIRAALLPALEGRTARTVIHVPFR
                     APNPIFAAIRDLPWHTLGLDLVEAPAGWELIPLFPEGRGVALGLIDARNTRLEDGDTI
                     AKLAERAARLRPDLDYQITPTASLEYLPADKAEAKIAVLVEAARLAGEGTS"
     gene            5949..6947
                     /locus_tag="VFM00_09905"
     CDS             5949..6947
                     /locus_tag="VFM00_09905"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET7498602.1"
                     /translation="MMPRGLWTTTVGSFPKPPHLERARQQHARGEISADRLFAMEREE
                     TAKVIRQQEEVGLDILVDGELYRGDMTTYFAERMPGFAISNPVRSYGNRYYRKPIAVG
                     PIRRDQELTVSWWTYAQSLTRKPIKGMLTGPYTMMDWSFNEHYPSREAMAMDLAIAVN
                     EEALALQEAGARFIQIDEPAVSVRPEELPLAVKALGRAVQGLTAKTITHICYGDFDVI
                     YPALLDLPVDMIDLEMANSRYDLLERFRVNPFTKEIGYGVLDVHSHRVETKEEVKEGI
                     RRGLEVLRPEQMYVDPDCGLKTRTVEEAFAKLRVLVDAVREVRAELGIEAEQGVTA"
     gene            6944..8221
                     /locus_tag="VFM00_09910"
     CDS             6944..8221
                     /locus_tag="VFM00_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7498603.1"
                     /translation="MTDSRSADLSGLRIDRSAKRPQGGNRARTLALLAALAGILTVVI
                     LFGVRRSRATEVRVAPVEAVGGAPGAAAAEVLTANGYVVARRRASVSTEVAGRLEALY
                     VAEGSRVREGQMLGVLRNDEQRAAVRSAQADLSTARAARAEAEASAYEAGLARKRLQA
                     LLTKGLVSQSDFDEADARAKVADARVQSAAASMENARARLSAAQVDYARTFIRAPFSG
                     AVLRKEAEVGEIVSPIPSSGGLTRGAIVTMADLSTLEVDVDVNEGYVARTHEGMRAEI
                     ALDAYPGDRYPGRVRQIVPTADRQKATVLVKVTFDSLDARVLPEMGAKVTFLADQEPA
                     AAGTHVVTAAVTIPKGAVADRGGRAVVFVVEKGRAALRSVGPRPYGADRVVVSGGLAP
                     GELVVLDPPAALADGSRIKPAGGKDVNWGEHGW"
     gene            8211..8906
                     /locus_tag="VFM00_09915"
     CDS             8211..8906
                     /locus_tag="VFM00_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7498604.1"
                     /translation="MAGDATQDGALIRLRNVHKRYRREQNVLDVLAGIDLDVKAGEFL
                     ALMGPSGSGKSTLLNLIGGIDRPSEGEVIVAGESLAALSSNQLAAWRARNVGFIFQLY
                     NLIPVLTAQQNVELPLLLTRLSAKERRERVALALRLVGLEERSGHFPRQLSGGEEQRV
                     AIARAIVADPTLLVADEPTGDLDARNADEILTLLQRLNQEMGKTIIMVTHDPHAASRA
                     HLTRHLEKGVLQA"
     gene            8903..>9782
                     /locus_tag="VFM00_09920"
     CDS             8903..>9782
                     /locus_tag="VFM00_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7498605.1"
                     /translation="MKFLRYVLAHMARAKRRTFLTVSSIALALFLFCTLRTVMTSFNA
                     AIRASDDTRLVVRHAASLVFPLPLAYRDRLTQVPGVQEVGFGNWFGGFYQDPKNQFAQ
                     FAVDTDHFFGIYPEVLVPPDQREAFLRERQGCLAGADIVKKYGWKLGQTIPITGTIYP
                     GDWRLTLRGIYTGRTEDIDQNTIFFHWDYLNESMPDVRKNQVGIFWLRLQSASEAPAV
                     SDRVDAMFENSPQPTKTETEKAFQAGFISMLGNVSMLLTILGSAIVFAIVLVTINTMM
                     MSARERTTEIAVLKTLG"
BASE COUNT         1456 a         3412 c         3484 g         1430 t
ORIGIN      
        1 gcgccagcgg atgctcggag cccgcctcga cggccgccgc cgccgcgagc atgcgcgcgg
       61 ggtcgatccc gggcgccgcg gccaggctct ccaccaccgg ctcgccgcgc gtgagcgtgc
      121 cggtcttgtc gaagacgacc gtggtgagcg agcccgcgcg ctccagcgcc tcgccgcccc
      181 ggatcaggat cccgcgccgc gctccggcgc ccgttccggc gaggatggcg gtgggcgtgg
      241 cgagccccat ggcgcagggg caggccacga tgaggacgga gacgaagacg gtgagcgcgt
      301 agcgcgcacg gggctcggga cccacgaaga gccagatcgc cgcggccgcg atcgcgatcg
      361 cgatcacggc ggggacgaac cgcgccgcca cgcggtcggc cagctcctgc atgggcgcct
      421 tcccgccctg cgcctcctcg accagccgcg cgatccgcgc gagcgtggtc tcggcaccca
      481 cccgcgtcgc gcggaggatg aagggagcga tccggttcac cgtgcccgcc gcgaccggat
      541 cccccggctc gcgcgcgacg gggaagggct cgcccgtgag catcgattcg tccacttcgg
      601 tgcgcccctc ggcgatcagc ccgtccaccg ggatgacctc gcccggaagg atctcgagga
      661 gatcgccgcg ggcgaccgac tccacgggaa cggactcgcg cggcgcgtcg aaccccggct
      721 cgcctccggc cgcctcggcc gtgaggcgcc gcgccgtcgg aggccgcctg gtgagcagcg
      781 ctttcagcgc gtctccggtg cggcgccggg cgcgcgactc gaggaggcga ccgagcagga
      841 tgaagacgag gatcatgacc gccgtgtcga agtagacgtg cgtgcccagc cccgatgcgc
      901 ggaagcgtcc gggcgcgagg gtcacggcgg cggaatagag gtacgcggag aaggtgccca
      961 gcgtgatcag ggtgttcatg tcggcggtcc gatggcggag cgccttcacc gcgcgcgcgt
     1021 ggaacggcca ggccgcgatc cactgcacgg gggtggcgag gaggagggcg agccagcgcg
     1081 cgcggtcctg atcgtgcacg aagaggtgga cgaccatgat cggcacgccc agcgcgacgg
     1141 agaggagaag cctcccgcgg agcgcccgct cctcggccag gcggaccttc tcccgctccg
     1201 aagaagaggt cgaggcgcgg ggcgcggcgc ggtagcccgc gcgctccacg gcactcacca
     1261 gcgcggcgac gctgggcttc gtgccgggca ccacgcgcac gcgcgcctct tccgtcgcga
     1321 gattgacctc ggccgacgcc acgccggcct cgccccgaag ggcgcgttcc accgtggcga
     1381 cgcatcccgc gcaggtcatg ccgtgcacgg tgagcacaac gtccccttcg cgcgccggtc
     1441 ccgggtcggc ggccggcttc gcgggcggtg cggcggtcgc accgcccgtc gccgtgaggt
     1501 gcatcgtttg ccgcggcatc gcgtctacct cagagagggg gattcggaaa tgacggaagc
     1561 ggcgtttgga cgcccgtttc ctgtcccgta tggattatga tgtcgagtcc gatcccgccc
     1621 ccacacaccc atcgaggagc gatgatggcc caggcccctg tctcggaagc ccaagtcttg
     1681 gatgcccttc ggtccgtcat ggatccggat ctccaccgcg acatcgtctc cctgggcttc
     1741 atccggaaca tgaagatcga gggggggacg gtggcgttcg acgtgaatct gaccaccccc
     1801 gcctgccccg tcaaggatcg cctgagggac caggcccgtc aggccgtact cgcgatcccc
     1861 ggtgtctccg acgcccgcgt gaacatgacc gccgaggtgc gccgcccgca ggccccgagc
     1921 acggccgcgc tccagaacgt gaagaacatc gtcgcggtcg ggagcggaaa gggaggcgtc
     1981 ggcaagtcca ccgtcgccgc caacctggcc gcttcgctcg cgcggagcgg cgcgcgcgtg
     2041 ggactcctgg acgccgacat ctacggccct tccatcccga tcatgatgcg cgggtcgcgt
     2101 cccgccgagc cgcgcgccga caacaccatc gagccgggcg acgcgcacgg cacgaaggtg
     2161 atgtcgatgg gctatctcac gcccggggac cagccgctca tctggcgcgg cccgatggcg
     2221 cacaaggcgg tccagcagtg cctgctgggg gtgaactggg gggagctgga ctacctgatc
     2281 gtggacctgc cgccgggcac gggggacgtg cacctcacgc tggtacagac ggtgccggtc
     2341 accggcggcg tgatcgtctc gacgccgcag gacgtcggcc tccagatctc gatgaagacg
     2401 ctccgcatgt tccagcagac caaggtgccc atcctcggca tcgtcgagaa catgtcgtac
     2461 tacgtctgcc cgcactgcgg cggacgggac gatctcttcg gccacggcgg cgccgaggaa
     2521 gcggcgaagg cgctggacgt gcccttcctg ggctcgatcc cgctcgaccc cgcgatccgc
     2581 cgcgcggccg acgaggggac gcccgtggtg gtcgccgagc cggagtcgcg ctcggggaag
     2641 gcgatggccg acgtggccgg cgcgctcgcg cgccaggtga gcatccgcgc gttccggaac
     2701 ctcacgctct cgatcgtgga ggagtgatgg cccgcgttct cctgcccgag gagaccaccc
     2761 ccgtcgcgct gaagaagaag gggggcgagc gcttcacgat ccgctgggag gacgggcacg
     2821 agtcggtcta ctccgcgcgc acgctgcgcg gccgctgtcc gtgcgcccag tgcgtcagcg
     2881 agacgacggg ggagcgtatg gtgttcgagg agcacgtgga tcccggcgtc tcgatcgccg
     2941 ccgcgcgcac cgtcgggaac tacgcgctcc acttcgactg gagcgacggc cacacgaccg
     3001 ggatctacgc cttcgactac ctccgccgca tctgcccgtg cgccgcctgc acgggccagg
     3061 cgggctgacc cgccatccgc gcgctcgacg ggcctctcgg gagcttccgg aggctcgacg
     3121 attggaagcg caccctcttc gcgctgtgga cggcccagtt cttcaccatg ctcggcatga
     3181 gcatgttcct gccgttcctt cccctttacc tgcgccagct gggcgtcacc gagccgggga
     3241 agcttgagtt cgtcagtggg ctcttattcg cggccccctt cttcgccgcg acggtcgcga
     3301 cgccggtatg gggcgtgctg ggggacaagc acgggcggaa gctgatggtg gtgcgcgcca
     3361 gcctcgggct ttcgctcgcg accctgctga tgggctttgc gcggacggtg ccgcagctct
     3421 tcgtgctgcg ggtggcgcag ggcgccgtct ccggcttcat cgcggcggcg ctcgcgctga
     3481 tggcgtccaa ggcgccgcgc gagcggatgg ggtatgcgct cggcacgctc cagaccgcga
     3541 ttcccaccgg caccatcctc ggccccttga tcggcggcgc cctcgccgac gcgatcggct
     3601 accggcagat cttcttcgtg accgcggcga tgaatctgac ggcgggcatc gcggtgatcc
     3661 tcctcgtgcc gcgcgatctt cccgagccgc gcgaggccgg gttcggacag gtcttcgaga
     3721 actacaggac ggtgttcggg acgccgcagt tccggctcct cttcttcgtc ctcttcggaa
     3781 gccagttcgc gctgatgtcg atccagccgg tgatggcgct ctacgtggaa taccttggga
     3841 cgagcggacg gctcctcgcc acgacgaccg gggtgatctt cgcgatcacg ggtgtcgcga
     3901 gcgcgatcgg cgcgccacgg tgggggcggc gcggcgaccg gaagggctat cgccccgtgc
     3961 tcggccgcgc cctcctcggc gccgccctgt tcgcgctccc gcaggcgctg gtcgcgcgcg
     4021 cctggcagct cttcctgctc cggatcggct acggcgcctt catcggcggg atcgtcccct
     4081 cggtgcaggc gatgatcggc attcgggcgc cggccgagcg ccgcgccggg atcatggggg
     4141 tcacctcgac cgcgctcatg atggggaatc tgaccggccc cttgatcggc ggcgcggtgg
     4201 cgggaagctt cggtctgcga tcggtcttcg tgctcgccac cgcggtcttc gtcgcgctcc
     4261 tcgcggcgct ctacccgcgc atggcggagc cggcccggcc ggagaccgtg cgacccgagc
     4321 ccgcggccca ggtcgtcacg gtctgagaag ccgggaggcg tacgccctcg atcagcgctt
     4381 cgtagtctcg aggatggaga ggatgttgcc ggccgggtcc ttgaaccagg cgatgaccgg
     4441 acccttgccg cggtggatcc ccttggcgtc cgtctggatc tcgccgccgt actgttcgaa
     4501 cggcacgcct cgggccgtca gctcatcgac catctcctcg acgctcgcga ccgggaaatt
     4561 gaggatcgtg aaggtggccg gctggtggtt cggcttggga taggccagga ccacggcgcc
     4621 cccggggaga tgaaggtgga gcatgccgtt ctcttcccgg acgtcgagac ccagggtctc
     4681 gccgtagaac gccttggcct tagggatgtc gtcgacggaa aagccgctga acgcgcggct
     4741 ctcgcgcagc agagctcgtt cgctggtagc ggcgcgtgat ggtgaggtat cgcgggtcat
     4801 gaccgcctcc ttcgcgtcgc gtgcccggta cgccgggccc gacgtgggtg gaccggcgtg
     4861 gcgctgcaag cagcctgcca tttccggcgc cgccactcct tgtcggctaa cggcttctct
     4921 agtagcatcc tggactcatc gcgaacttcc acccgcccag gaggaaccgt gcaattccgg
     4981 acgacgctcg cggggagcta tcccaagctc ccaacccgct cgggggacgt gaacctgcgc
     5041 gtgacgctga accgccgcga ccaggggaaa gccacggatc aggacgtggc cgacgcgatc
     5101 cgcgcgacga cgcggcgcac gctggcgatg gaggagcaag ccgggctcga cctcctcatg
     5161 gacggaggcg ccgggtggga ggacgcccag acctacgtgg cgcgcggtct cgagggcatg
     5221 cgcatcgcgg ggctgatccg ctatctcgac accaacacct actaccgcca gcccgagatc
     5281 gtcggtcccg tggcgtggaa gggaccgatc acggtcggcg actacctggc cgcgacggcc
     5341 atgacgaaga agccggtcaa ggcgttcctg ccggggccct actcgctcta ccggttctcg
     5401 aaggacctcc actacggcga cgccgtggcg gccggcgccg cgatcggcaa ggccctggcg
     5461 cgcgaagccg ccgcgctcga agaggccggc gcgtcctgga tccacttcga ggagccgtgg
     5521 ctggggcggg cacgcgcgga agacgcttcg gcgatccggg ccgcgcttct gcccgcgctg
     5581 gaggggcgga ccgcccggac ggtgatccac gtgccgttcc gcgcgccgaa tccgatcttc
     5641 gccgcgatcc gcgaccttcc ctggcacacg ctgggcctcg acctggtcga agcccccgcg
     5701 ggctgggagc tgattccgct ctttcccgag gggcgcggcg tcgcgctcgg tctgatcgac
     5761 gcccgcaaca cgcggctgga ggacggcgac accatcgcca agctcgcgga gcgcgccgcc
     5821 cggctgcgcc ccgacctcga ctaccagatc acgccgacgg ccagcctcga gtacctgccg
     5881 gcggacaagg cggaggccaa gatcgccgtg ctcgtcgagg cggcccggct cgccggagag
     5941 gggacgtcat gatgccgcgc ggactctgga ccaccaccgt cggaagcttc cccaagccgc
     6001 cgcatctgga acgcgcgcgc cagcagcacg cgcgcggcga gatctcggcc gaccggctct
     6061 tcgcgatgga gcgcgaggag acggcgaagg tgatccggca gcaggaagaa gtggggctgg
     6121 acatcctggt ggacggcgag ctctaccgcg gcgacatgac gacctacttc gccgagcgga
     6181 tgcccgggtt cgccatctcg aacccggtgc gctcctacgg caaccgctac taccggaagc
     6241 cgatcgcggt cggaccgatc cgccgcgacc aggagctgac cgtctcctgg tggacgtacg
     6301 cgcagtcgct gaccaggaag ccgatcaagg ggatgctcac cggcccctat acgatgatgg
     6361 actggtcgtt caacgagcat tacccctcgc gcgaggcgat ggcgatggac ctggcgatcg
     6421 cggtgaacga ggaggcgctc gcgctgcagg aggccggggc ccgcttcatc cagatcgacg
     6481 agcccgcggt ctccgtgcgc cccgaggagc tcccgctcgc ggtcaaggcg ctggggcgcg
     6541 ccgtccaggg cctcaccgcc aagacgatca cccacatctg ctacggcgac ttcgacgtga
     6601 tctaccccgc gctgctcgac ctcccggtcg acatgatcga cctcgagatg gcgaacagcc
     6661 gctacgacct cctggaacgg ttccgcgtga acccgttcac caaggagatc ggttacggcg
     6721 tgctcgacgt ccacagccac cgcgtcgaga cgaaagagga agtgaaggaa gggatccgcc
     6781 gcgggctcga ggtgctccgc cccgagcaga tgtacgtgga tcccgactgc gggctgaaga
     6841 cccgcaccgt cgaagaggcg ttcgcgaagc tgcgcgtcct ggtggacgcc gtgcgcgagg
     6901 tgcgtgccga gctggggatc gaggcggagc aaggcgtcac ggcgtgaccg attcacgctc
     6961 ggccgatctg tccgggctcc gcattgatcg ttcggcgaag cgtccccagg gggggaaccg
     7021 cgcccggacg ctcgcgctgc tggccgccct ggccggcatc ctcacggtcg tcatcctgtt
     7081 cggcgtgcgt cgttcccgcg cgaccgaagt gcgcgtggct ccggtcgaag cggtcggggg
     7141 cgcacccggc gccgcggctg cggaagtcct caccgccaac ggctacgtcg tcgcccgccg
     7201 ccgcgcctcc gtttccaccg aggtggcggg acggctcgag gcgctctacg tcgccgaggg
     7261 gagccgcgtc cgggaagggc agatgctggg cgtgctccgg aacgacgagc agcgcgccgc
     7321 ggtgcggagt gcccaggccg acctgagcac ggcacgcgcc gcgcgcgctg aggcggaggc
     7381 cagcgcgtac gaggctgggc tggcccgaaa gcgcctccag gctctcctga cgaagggcct
     7441 ggtatcgcaa tccgatttcg acgaggccga cgcgcgcgcc aaggtggcgg acgcgcgcgt
     7501 gcagagcgcg gccgcgtcca tggagaacgc gcgggccagg ctctcggcgg cgcaggtgga
     7561 ctacgcgcgc accttcatcc gggccccttt ctccggcgcc gtgctccgga aggaggccga
     7621 ggtgggggag atcgtgagcc cgatcccctc cagcggaggc ctcacgcgcg gggcgatcgt
     7681 cacgatggcc gacctctcca cgctggaggt cgacgtggac gtgaacgagg gctacgtggc
     7741 gcgcacgcac gaggggatgc gggccgagat cgcgctggac gcctatcccg gcgaccgcta
     7801 ccccggccgc gtgcgccaga tcgtgcccac ggccgaccgg cagaaggcga cggttctcgt
     7861 gaaagtgacg ttcgactcgc tggacgcgcg cgtgctcccg gagatggggg ccaaggtgac
     7921 gttcctggcg gaccaggagc ccgcggccgc cgggacgcac gtcgtcacgg cggcggtgac
     7981 gatccccaag ggcgccgtcg ccgatcgcgg cggccgcgcc gtggtcttcg tggtcgagaa
     8041 agggcgcgcc gcgctccgga gcgtcggccc tcgtccgtac ggggcggacc gcgtggtcgt
     8101 gtcgggcggg ctggcgcccg gcgagctggt ggtgctggac ccgcccgcgg cgctcgcgga
     8161 cggatcgcgg atcaagccgg cgggcggcaa ggacgtgaac tggggggaac atggctggtg
     8221 acgcgaccca agacggcgcc ctgatccggc tccggaacgt gcacaagcgc taccggcgcg
     8281 aacagaacgt gctcgacgtg ctggccggaa tcgatctgga cgtgaaggcg ggggaattcc
     8341 tcgcgctcat ggggccgagc ggctcgggaa aatcgacgct cctgaacctg atcggcggca
     8401 tcgaccgccc gagcgagggg gaggtgatcg tggcggggga atcgctcgcg gcgctctcct
     8461 cgaaccagct cgcggcgtgg cgcgcgcgga acgtcggatt catcttccag ctctacaacc
     8521 tgatcccggt gctcacggct cagcagaacg tggagcttcc gctgcttctc acgcggctct
     8581 ccgccaagga gcggcgggag cgggtggctt tggcgctccg tctggtcggg ctggaggagc
     8641 gctccggcca cttcccgcgc cagctctcgg gaggcgagga gcagcgcgtc gccatcgcgc
     8701 gcgccatcgt ggccgacccg accctcctgg tggcggacga gccgaccggg gacctggacg
     8761 cgcggaacgc cgacgagatc ctgacgctgc tccagcggct gaaccaggag atggggaaga
     8821 cgatcatcat ggtgacgcac gacccgcacg ccgcatcgcg ggcgcacctg acgcggcacc
     8881 tcgagaaggg agtgctgcag gcgtgaagtt cctgcgctac gtcctggcgc acatggcgcg
     8941 cgccaagcgc cgcacgttcc tgaccgtctc ctcgatcgcg ctcgcgctct tcctcttctg
     9001 cacgctccgc accgtgatga cctcgttcaa cgccgcgatc cgcgcgtcgg acgacacgcg
     9061 gctcgtggtg cggcacgccg cctcgctggt ctttccgctg cctttggcct atcgcgaccg
     9121 gctgacgcag gtgcccggcg ttcaggaggt cggcttcggc aactggttcg gcgggttcta
     9181 ccaggacccg aagaaccagt tcgcgcagtt cgccgtggac accgaccact tcttcggaat
     9241 ctatcccgag gtgttggtcc cgccggacca gcgcgaggcg ttcctccgcg agcggcaggg
     9301 gtgcctggcc ggcgccgaca tcgtgaagaa gtacggctgg aagctggggc agacgatccc
     9361 gatcaccggg acgatctatc ccggcgactg gaggctgacg ctgcggggga tctacaccgg
     9421 ccgcaccgag gacatcgacc agaacacgat cttcttccat tgggactacc tgaacgagtc
     9481 gatgcccgac gtgcgcaaga accaggtcgg catcttctgg ctgcgcctcc agtccgcgtc
     9541 cgaagcgccg gccgtgagcg accgcgtgga cgccatgttc gagaacagcc cgcagcccac
     9601 caagaccgag accgagaaag cgttccaggc gggattcatc tccatgctcg gcaacgtgag
     9661 catgctgctc accatcctcg gttcggcgat cgtcttcgcg atcgtgctcg tcacgatcaa
     9721 cacgatgatg atgtcggcgc gcgagcgcac cacggagatc gcggtgctga agacccttgg
     9781 ct
//
LOCUS       DASSLZ010000263         3605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_477032, whole genome shotgun sequence.
ACCESSION   DASSLZ010000263 DASSLZ010000000
VERSION     DASSLZ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477032"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VFM00_09925"
     CDS             complement(<1..786)
                     /locus_tag="VFM00_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET7498606.1"
                     /translation="MAAVMTPNATPFLWGVATSAHQVEGGNDRNDWWDWEQTPGHIRG
                     GARSGSACLGWERYEEDLDLVRALGLNAYRFSLEWSRIEPDPGRYDESAIAHYRAVLE
                     GCRARGITPLLTLHHFTNPRWFAALGGWEERANLPHFERFARLAGERYGDLVDRWVTV
                     NEPEVYGFYGYASGIWPPGVSDRSRALQVIANMLEGHALAARALRDTDRVDADGDGRA
                     TWIGASKHWVLLDPKRPWWPPDRLAASAQHQVFNVAVARALAGG"
     gene            920..2011
                     /locus_tag="VFM00_09930"
     CDS             920..2011
                     /locus_tag="VFM00_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7498607.1"
                     /translation="MPSTAPIRVLIAGSGFGAKVHAPGFAQNPDFRIVGIASGHLETA
                     RAAADAHGIPYATDDWKKMLDEVEADLVSIVTPVDLHYPIARAALERKRHVLCEKPFA
                     MNTAQAKELASLAKAQGVVNVVNHEFRYMPARALMSRMIQEGKLGRLEHIAIQDRLPG
                     WARDPSRRITWLADRQRGGGFLGALGSHHVDAITHWAGPVRRVFCRLRTLAATAPGLP
                     PGHQAITADDCFTLMLELESGATAVVDLFAGSRARRESIEAFGSDDALVIEEARRIGR
                     RTSQGTLEELPIPGDLDIPTTPEMPLLAPFRVMVAKLRDAIRGGAPLGPTFAEAVEIQ
                     KVLDTARLSDQKGGWLSTRSATAARASAG"
     gene            complement(2045..>3605)
                     /locus_tag="VFM00_09935"
     CDS             complement(2045..>3605)
                     /locus_tag="VFM00_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7498608.1"
                     /translation="QLPPGSVCLQVAPTYAHEFPMPLLPIGQHLVTAVERVVDENGAL
                     IDSSTADLSFTVAGADSCWPCVVLGFAAPPPGNPVGCDAAALPGGSACFDISLGNNIP
                     VGAAQLRIQITDDQGQPLSAGSFTPVSVSRLDRAGGMEMEWAADGSATGIILYSAGTG
                     SIGQGHEPILRVCYSVGPDVPQGSYSIVFEKSIVLDVHGRTLGLCPTFRMASGRFCVG
                     ATSGCDVNEDGVADVTDIVAIVRCALAGDACVPAVAARSDCNGDGAVDVRDVICCVRK
                     LLQLQVHAVNAPSDFSAPHTGVAFSGAARWTSPLSGEASIDVTPVPQFGATVFDLIAP
                     DGIRITSLDLGGGMGYRLEWADRGGGTVRAMLLREGSAPVGPARITLGFERTGAGGLE
                     ASSLRIQNASGSTWDAVPTIIETPVDRAPVAAQAIPAPTVYRARPNPFSGATEIAFAI
                     PAAGRVSLRVYDAAGRLVRTLVDGAREAGVARVSWDGRDQAGRGARSGIYFVRLSAAG
                     AERSVRIVKLR"
BASE COUNT          569 a         1292 c         1269 g          475 t
ORIGIN      
        1 ccctcccgcc agcgcgcgcg ccaccgcgac gttgaacacc tggtgctgcg ccgaggccgc
       61 gagccggtca ggcggccacc agggacgctt gggatcgagc agtacccagt gcttcgaggc
      121 gccgatccag gtcgcgcgac cgtcgccgtc ggcgtccacg cgatcggtgt cgcgcagcgc
      181 ccgcgcggcc agagcgtgcc cctccagcat gttcgcgatc acctggagcg cgcgcgagcg
      241 gtcgctcacg cccggaggcc agatgccgct cgcgtagccg tagaagccgt aaacctccgg
      301 ctcgttcacg gtgacccacc gatcgaccag gtcgccgtag cgctctcccg cgaggcgcgc
      361 gaagcgctcg aagtggggca ggttcgcccg ctcttcccat ccgccgagcg ccgcgaacca
      421 gcgcggattg gtgaagtgat gcagcgtgag gagcggcgtg atcccacgcg cccggcagcc
      481 ctcgagcacc gcgcggtagt gcgcgatcgc cgattcgtcg tagcgtcccg ggtccggctc
      541 gatccggctc cactccaggg agaagcggta ggcgttcagc cccagcgcgc gcaccaggtc
      601 gagatcttcc tcgtagcgct cccatcccag gcaggcggat cccgaccggg cgccgccgcg
      661 gatgtgtccg ggcgtctgct cccagtccca ccagtcgttc cggtcgttgc cgccttcgac
      721 ctgatgcgcc gaggtggcaa cgccccagag gaatggagta gcgttcggag tcatgacggc
      781 ggccacgttc catcccagcc cctgccgcgc gcaagcggga atttgcggac gccgatgagc
      841 gatccggcgc accaggcgcg cgcggtcgcc ggaatgtccg agtaccccgg ccggagctga
      901 accgctacaa tccccggcca tgccttcgac cgcccccatc cgcgtcctca tcgccggctc
      961 cggcttcggc gccaaggtgc acgcgccggg cttcgcccag aatcccgatt tccggatcgt
     1021 ggggatcgcg agcggacatc tcgaaaccgc gcgcgccgcg gccgatgcgc acggcatccc
     1081 gtacgcgacc gacgactgga agaagatgct ggacgaggtg gaggcggatc tcgtgagcat
     1141 cgtgacgccg gtggatctgc actatccgat cgcccgcgcg gccctggagc ggaagcgcca
     1201 cgtgctctgc gagaagccct tcgccatgaa caccgcgcag gcgaaggagc tcgcgtcgct
     1261 cgccaaggcg cagggcgtgg tgaacgtcgt gaatcacgag tttcggtaca tgcccgcgcg
     1321 cgccctgatg agccgcatga tccaggaagg gaagctgggc cggttggagc acatcgcgat
     1381 ccaggaccgg cttccggggt gggcacgcga tccgtcccgt cggatcacct ggctcgccga
     1441 ccgccagcgc ggaggagggt tcctgggcgc gctcggctcg catcacgtgg acgcgatcac
     1501 gcattgggcg gggcccgtgc gccgcgtctt ctgccgcctc cgcacgctgg ccgccaccgc
     1561 gccggggctg ccgcccgggc accaggcgat caccgccgac gactgcttca ccctcatgct
     1621 cgagctggag tcgggcgcga ccgcggtggt ggacctcttc gcggggagcc gcgcgcgccg
     1681 ggagagcatc gaggcgttcg gctcggacga cgcgctggtc atcgaggagg cgcgccggat
     1741 cggccggcgc acgtcccagg gaacgctgga agagctgccg atccccggcg atctcgatat
     1801 cccgaccacg ccggagatgc cgctcctcgc cccgttccgc gtgatggtgg cgaagctgcg
     1861 cgacgcgatc cggggagggg cgccgctggg cccgacgttc gccgaggcgg tcgagatcca
     1921 gaaggtcctg gacacggccc gcctcagcga tcagaagggc ggctggctca gcacccgatc
     1981 agccacagcg gcgcgagcat cagcaggatg aagatcagcc actgaacgac cgggttgtgc
     2041 ggactcaacg cagcttgacg atccgaacac tccgctccgc gcccgccgcg ctcagccgga
     2101 cgaagtagat ccccgaacgg gctcctcgcc cggcctgatc cctaccgtcc caggaaaccc
     2161 gcgccacgcc ggcctcgcga gcgccatcga ccagcgtgcg cacgagccgc cccgccgcgt
     2221 cgtagacgcg aagggagaca cgtcccgccg cggggatcgc gaacgcgatc tcggttgctc
     2281 cggagaacgg attcgggcgc gcgcggtaga cggtgggcgc gggaatcgcc tgcgccgcga
     2341 ccggagcgcg gtccaccgga gtctcgatga tcgtcgggac cgcgtcccac gtggagcccg
     2401 aggcgttctg gatccggagc gatgacgcct cgagaccgcc ggcgccggtg cgctcgaagc
     2461 ccagggtgat ccgcgccggt ccgaccggag cggagccctc gcgcagcagc atcgcacgga
     2521 ccgtcccgcc gccgcggtcc gcccactcga ggcgataccc cattcctccg cccaggtcga
     2581 ggctcgtgat gcggattccg tccggcgcga tcaggtcgaa caccgtggcg ccgaactggg
     2641 gcaccggcgt gacgtcgatc gaggcctctc cggacaaggg tgaggtccac cgagccgccc
     2701 ccgagaacgc aacgcccgta tgcggcgcgg agaagtcgga cggggcgttc accgcgtgga
     2761 cctggagctg gagcagcttc cgcacgcagc agatgacgtc ccgaacgtcg acggcgccgt
     2821 cgccgttgca gtcggagcgc gcggcgaccg ccgggacgca ggcatcgcct gccagcgcgc
     2881 atcgcacgat ggcgacgatg tcggtcacgt cggcgacgcc gtcctcgttc acgtcgcagc
     2941 cggacgtggc gccgacgcag aaccgaccgg aggccatgcg gaaggtgggg cagagtccca
     3001 gcgtgcggcc atggacgtcg aggacgatgg acttctcgaa cacgatcgag tagctgccct
     3061 ggggcacgtc cggcccgacc gagtagcaga cccggagaat gggctcgtgt ccctgcccga
     3121 tcgaaccggt gccggccgag tacaggatga tcccggtggc ggacccgtca gcggcccact
     3181 ccatctccat cccgcccgcg cggtcgaggc gcgacaccga gacgggcgtg aaggagcccg
     3241 ccgaaagcgg ctgtccctgg tcgtccgtga tctggatgcg gagctgcgcg gcgccgacgg
     3301 gaatgttgtt gccgagggag atgtcgaagc acgcggaccc gcccggaagc gccgcggcgt
     3361 cgcaacccac ggggttgccc ggcggcgggg cggcgaagcc gagcaccacg catggccagc
     3421 acgaatcggc gccggccacc gtgaaggaaa ggtccgccgt gctcgagtcg atcagggctc
     3481 cgttttcgtc gaccacgcgc tcgaccgccg tgaccaagtg ctgcccgatc gggagcagcg
     3541 gcatcggaaa ctcgtgggcg tatgtcggcg ccacctggag gcacacgctc cccgggggca
     3601 gctgc
//
LOCUS       DASSLZ010000264        17143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1617137, whole genome shotgun sequence.
ACCESSION   DASSLZ010000264 DASSLZ010000000
VERSION     DASSLZ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 17143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17143
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617137"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFM00_09940"
     CDS             <1..238
                     /locus_tag="VFM00_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498609.1"
                     /translation="SAALVLTQPVEHSLAAAAKRPVPPAPESPAMTAATRKEIASQKK
                     SVADMLKVIRAYKLPPGSRPAFLFRAEKRSVSRA"
     gene            231..1646
                     /locus_tag="VFM00_09945"
     CDS             231..1646
                     /locus_tag="VFM00_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7498610.1"
                     /translation="MPDLHADLCFQSVAQLSDLIRRRKLSPVELTEAYLDRIRRYAAE
                     MNAFVTVTDDIAMEQAREAEREIQRGRRRGPLHGIPYGAKDLLATAGIKTTWGAAPTR
                     SQMFDRDATVIRKLREAGAVLLGKLGMVEFAGCLGYRFANASLTGPGRNPWDLNRWTG
                     GSSSGSGAAVASGLVGFAIGTETWGSILCPSAFCGVTGLRPTYGRVSRAGGMVGSFTL
                     DKIGPLARSAGDCRLVLDAIAGPDPDDPSAADEPVRLRRRRLDPRSVRAALVPLDFAK
                     TAGAEPEVGAAFRAAVDELRGAGLRIEEAKLPEFPASEVATTVLYAEALSTFEKFFDD
                     GTVRQLTDPYAPYQREVTEALTGADYVKATRMRTLLQEKMLDFYSRYDVIVTPNFMSV
                     APPVAEDLVKTLPYADPVGAIGNACGLPAIALPAGFGRGHMPASFQVMGSAFEEGLLL
                     DLGELYQSRTSFHEARPPRYA"
     gene            complement(1660..3138)
                     /locus_tag="VFM00_09950"
     CDS             complement(1660..3138)
                     /locus_tag="VFM00_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7498611.1"
                     /translation="MAHDRGAAATAADPLLARLLELERLTAPLHPDAEERESLAEAAF
                     VHAEALADEVDRGVPSVRDHGDRAITEFPIGPEPVDAATVFSLLVRHVDALGMTPHAP
                     GFFGYIPGGNLYHAAIADFLAAVSNRFTGHFLGSPGAVRVENQVLRWLGATVGYPANA
                     SGSLASGGSLANLSAILAAREAANLRSEQVPRAVVYATEHVHHCVPKGLRIAGLGESP
                     LRRVPMDAGWRMDPAALERMIDEDARVGRIPWLIVASAGTTDAGAVDPLDAIADVAGR
                     RRVWLHVDGAYGGMFALAPEGRRILSGIERSDSVVLDPHKGLHIPYGVGAVLVKDARA
                     MRAAHRFDAAYVPDEAHEVEEPSPSELSPELSRPFRGLRIWLPLLLLGTRPFEAALEE
                     KLLLARYAHRRLLEIPGLEVGPEPDLSIVVFRMAPDRADADERNDRLARALEEDGRAF
                     LSTTRLSGRRFLRLAILASRTHKAHVDRAIDAIREKSEALAG"
     gene            complement(3161..3484)
                     /locus_tag="VFM00_09955"
     CDS             complement(3161..3484)
                     /locus_tag="VFM00_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498612.1"
                     /translation="MDKHERGWEIQEGRPDLPLPQWNGSYRGDSRRLPKVVQKLAKGR
                     PLTPEEKAELLWRFYELDMQRRSRSRGLKGVLLVLATLGVMAFMAAYPPPWLRQEIVR
                     FLGPG"
     gene            complement(3608..4129)
                     /locus_tag="VFM00_09960"
     CDS             complement(3608..4129)
                     /locus_tag="VFM00_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7498613.1"
                     /translation="MLLIARPAPGEFLPYYGRYIDLVGDDLLGAMRSGVDSTRGLLGG
                     LPEARGDFRYAPDKWSVKEVLGHVIDCERVFTYRALRFSRNDATPLPGFDENAWTPQS
                     GYASRTLSSLLDEHRAVRAATVSFFENLPEAMWGRCGTANDARMSVRAAAYVIVGHEL
                     HHQNVLRERYLKS"
     gene            4162..5661
                     /locus_tag="VFM00_09965"
     CDS             4162..5661
                     /locus_tag="VFM00_09965"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7498614.1"
                     /translation="MPFATLGLPPAVLKGVRAAGYTDPTPVQNRAIPLVLSGQDVIAA
                     AQTGTGKTAAFILPILSRLIEGPKRIRALVLTPTRELAAQVETNARDYARFTDVRVGV
                     VYGGVPLPPQERQLKAGVELLVATPGRLLDLHDRLALRLDFIEILVLDEADRMVDMGF
                     APDLRRILRLLPTERQTLLFSATMPPELNSVAKDALKNPARVDLAPPSRPAAGITQAV
                     YPVSRDKKTDLLDAMLSKDEASSVIVFARTKHGADRLAKLLVRRGHSVASIHGNRSQS
                     QRERALSDLRRGRVAVLVATDIASRGIDVEGITHVINYDVPHTPEDYVHRIGRTGRVD
                     AVGDAFTLMAPEEGKQMAAIERFLGRAIPRVTLPDFDYKGRPSEGRPREEHRHREDRA
                     GNGAPREHGAGGRERRGASYPGSSRESRHSGGATAGPRRGGGGGRRPGSGASASRSGG
                     SRNGGSGRGAGPESDRRRPDHAPPAGEAERHGRRPRKVTSAERMRRGRH"
     gene            5672..6592
                     /gene="rocF"
                     /locus_tag="VFM00_09970"
     CDS             5672..6592
                     /gene="rocF"
                     /locus_tag="VFM00_09970"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HET7498615.1"
                     /translation="MSFKPASIEIIGAPVDLGAGRRGVDMGPSAIRIADLESRLEALG
                     HSVSDHGDVDVMIPETLEVGAGVLRYKAAILAACESLRIEVERIHGAGRTPLVLGGDH
                     SIAIGSVAATSNHFDSRSQPVGLIWFDAHGDCNTPLTTPSGNIHGMSLAVLLGQGDSE
                     LVRLGGRAPKVKPQHAVVIGVRDLDPGEREMLRDSGVAVFTIRDVDERGMKTVVGEAI
                     RIAGSGTGGIHLSFDMDVLDPEEAPGIGTPVPGGITYREAHLAMELLCESGLVVAADF
                     VEVNPVLDTRNSTGALAVELALSLFGKQIL"
     gene            complement(6596..7600)
                     /locus_tag="VFM00_09975"
     CDS             complement(6596..7600)
                     /locus_tag="VFM00_09975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HET7498616.1"
                     /translation="MTALFGLGAGVLPVLLFLTALLLLDSYKLTARRAVLRSIGWGLI
                     AAGIAYVVNAGLLRAGLDPVLLRRYAAPVVEESLKAALLVVLIRRGRIGFLVDAAIHG
                     FAAGAGFALVENAYYAGAGATGAGVWIARGLGTAVMHGSATAIAGILAKSLTERRGVS
                     LRWFLPGLAAAIVLHSLWNHVVLNPLVSAGLLVATMPLVVAFVFERSERATEAWLGEG
                     LDGEVELLELIASGEFRHTHAGEYLESLRLRFPGPVVADMLCWLQIHLELGLRAKGIL
                     LARAAGIDLPVDPAVRAAFVEMKFLERSIGPTGKLAMLPLWRGSGRDLWQLQLLAERR
                     "
     gene            complement(7611..10751)
                     /locus_tag="VFM00_09980"
     CDS             complement(7611..10751)
                     /locus_tag="VFM00_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498617.1"
                     /translation="MTQAGLETPESIKRGGLVGGASSFVCPHGRPRSARRWWAAAIAA
                     LLLAAGPTPARAQLKVLETDDLRLVYPAPALSFLAPYAARCFENSIRFHERLFDYRPT
                     EKVNVILDDASDFGNAGVGVTPRNIMVVHIAPVNFVYETGPSNERINFTMNHEGVHVA
                     ALDQAAGMDRFFRGVFAGKVRDTSRHPESVLYSFLTTPRRAAPRWYHEGAAVFLETWM
                     AGGLGRAQGPYDEMVFRAMTKDGSRFYDPLGLESEGAKVDFQVGVNSYLYGTRFMTYL
                     AYEYGPESLMTWIARRPGSHRWFAGQFHKVYGRSLNDEWRRWIAWERRFQTANLDSIR
                     LHPVTGYRDLSDRPLGSVSRAFVDPASRSLIAAAYYPGAPASLVAVPLPAAEDAPAAI
                     AGAGPEAGGGTQAPPPSGPPRRLLEVKGPALYFVSSLAFDPETRTIFYTADNNEWRDL
                     CAYDLRTRKSRVLQKDARVGDLAWDAKEGTLWGIRHFNGVSTLVRMLRPYTDYAQVAV
                     FPFGRDLYDIDISPDGKMLAGSVAEISGRQTLRLWKTDSLIAGDTASVQLYDFGASIP
                     AGFVFSPDGRRLYGSSYYTGVSNIFRYDFDGDSMSVVTNAETGFFRPIPLGGDSLIVF
                     RYAGGGFVPAAIEGRPLQDVSAITFLGQALTERRPLVTTWRVPPPSTVPIDSLIRKEG
                     NYRAASSLGLASIYPIVEGYKDYTAAGLQAIVSDPLGLDAIDLSLSYAPARGLPADER
                     WHAALGAARAPWKLEARWNGASFYDLGGPTKVSRKGYGGTLGYARTLMEDAPKSLDLD
                     VAVSGWGGLERLPDYQNVSTSPGFDKLLSGSAGLRFRNVRSSIGSVDYERGWRGSLTA
                     ATDGVRFVRGGHAAWRGFPRAEGTLDLGVPLPAPHASVWLRSAAGLSPGDRDEPFANF
                     YFGGFGNNVIDYHDPKRYREAEAFPGADLNAIAGRNYAKTTLDLNLPPLRFRRAGSLA
                     LYATWLRASLFAGGLMTNLDDASSRTTAADVGIQADLRLQLFTVSPLTLSAGYARAFV
                     RGGPSDDEWMVSLKIL"
     gene            complement(10783..11691)
                     /locus_tag="VFM00_09985"
     CDS             complement(10783..11691)
                     /locus_tag="VFM00_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7498618.1"
                     /translation="MNGKRWIVVAGLAVAAGVGIAAMNGLIPPRSGVEGTIAGAKRYQ
                     VEQISDKDVGLSDPAVQAFLQSDLFHKISTDPNFGKTILVPAENGKLVLVPTENGKLT
                     LVPTENGKAVLVAVENGKTVRVPTENGKLILVPTENGKAILVPAENGKTVKVPTENGK
                     TILVPTENGKTILVPTENGKTVLVPTEFGKTILVPTENGKTILVPAENGKTILVPTEN
                     GKTVRVPTENGKLVLVPTENGKAVLVPTENGKTVRVPTENGKFVLVPTENGKTILVPT
                     ENGKTVSVPAPQELEKTASQSAQSGQ"
     gene            complement(11798..12535)
                     /locus_tag="VFM00_09990"
     CDS             complement(11798..12535)
                     /locus_tag="VFM00_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498619.1"
                     /translation="MKPRAEGENESDEAVARILDRVTLEDPPADLRQNVIRAIGTRPE
                     PARQGWFETFRTEFRQRLFPRVIPFAAGAAAGVIVFALASGSGLWRGTGSMPSDGAMA
                     PLVGGTGSVAGGKAGTKVDDQSFELGRANVRFEVFRSDDRAVVAVQADAADPVLVTVQ
                     LPPGPSRVERLVTVPSSRGGVEYGPGWVRIRQSGRDRAEIGLVVGSGAGDAPIRIAVE
                     ASGGTVRGALRASATPPTDGLAGPKTN"
     gene            complement(12532..13077)
                     /locus_tag="VFM00_09995"
     CDS             complement(12532..13077)
                     /locus_tag="VFM00_09995"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7498620.1"
                     /translation="MDGAREAELIRRSLDGDADAYGELVAAHQRVLFNVALRMTGNRE
                     DAQDVTQVAFIKAYQKLATFDRSRKFFSWVYRILWNETLNLVQRRPRHEALDEETPAA
                     QPSPERESEERDTSARIHEAIEELPPDYRQVILLRHFAQLSYQEMSRVIELPEKTVKS
                     RLFTARRLLGSILERKGVALT"
     gene            complement(13127..13411)
                     /locus_tag="VFM00_10000"
     CDS             complement(13127..13411)
                     /locus_tag="VFM00_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7498621.1"
                     /translation="MFEVRFEGEDRVRLVGRLDAAQADAALAALGRLQGASVADFSEL
                     EYISSAGIGVILETYKRLHQGGHTFLLTNLSPRIRSIFGYAGLDRVLRIE"
     gene            complement(13411..13758)
                     /locus_tag="VFM00_10005"
     CDS             complement(13411..13758)
                     /locus_tag="VFM00_10005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7498622.1"
                     /translation="MIAEFLRAEGLDAAHAFDLDLMAEELFTNMVKYGNGAEPVAIGL
                     ARDGDAVVLVLRDVGAPPWDVTRPRALPDAPLEERRAGGLGLHLVQRLADRLEYHHDG
                     HTGTVTVRKRVAT"
     gene            complement(13850..16516)
                     /locus_tag="VFM00_10010"
     CDS             complement(13850..16516)
                     /locus_tag="VFM00_10010"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HET7498623.1"
                     /translation="MFRSRFALLLSLALLGIRAAAFASTAAPGDTMSTVTLFANFSTD
                     VVGAAPNLALPGAPAGDFLTLNEASGTVRVNATIDGLTMAAQMKQQSGVGGVSLWGWP
                     AAPPAGTERVSLGWRSVAQDDNPINIVACTARGSNGNVIASVEYGSHGTLTWNGIAGA
                     PRTLPVVYQQNRNNAFRLDVNLLTSTVSLSIDGVAIAGFQDVPFAQPATDVARLGFEA
                     GGAAPQTFAVDDLYAVAFARTPDAAPVVTAPATASGAETASLTVTATAADPDGQPIAS
                     FTASPLPAGATFTANASNTSGTLAWTPDFTQAGSYAVTFTATNALSGSATTAITIADT
                     DRVPVVSAPPSAAVEELGHIDVPVSASDPDGNAIDALTADLSALPAGPAASFTASADN
                     TSGTLSWTPGLGQAGEYPVTFSATANGATSSATTEMFVAALGTSVTGRFTWTPQAGEE
                     GNYTVTFNATDLGGTSSLPVSLTVLPAGAALAGPQRAAAAPGALAPQAPMKGPIISYS
                     GSTSTSTGTTLTGTTTATSGTTLLAGRLQRASSTTASSQAAQQGIITLTADLSGLPTG
                     NDAQFVVDRDPIVSAPALKQGDAGLPLTVNVGADDPDADPILTLTADWSALPSGNNAT
                     FTTNASHTLGTLAWTPTLADSGSYTVTFQATNRLVGSASTVIHVRGAAATRVFTVGAR
                     KLNLGSGKPYYQLAIEPIDGSFDLMDVDLTSIVLISEGTGTVSQIGTAAKPAPSVGDA
                     DNNKIEDMTLYFTKTDLRQLFSLLRGSVSVPVAIQGNLLTGGRFRGTASIDVNAGGGK
                     LAATVIPNPLNPEAVLRFVTSRSGPVSVRLYDVSGRMVRELLPRGPLASGEHQITIRG
                     EDSSGRRLASGVYFYRIEAAEGTTEGRLAVVK"
     gene            complement(16657..>17143)
                     /locus_tag="VFM00_10015"
     CDS             complement(16657..>17143)
                     /locus_tag="VFM00_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7498624.1"
                     /translation="LNGLGGVAFYRGDLESSGRYHEEALPVQRELGDTRGTAFSLREL
                     GEVATGRGDLGAAADRLREALTIYRDLEDRQGIASTLEGFAVLAAAHGRGERAFRLLG
                     AAGAIREAIQTPISGPDESRMERFLAPAREALGERVAAELSEGSKLALEPAMKLAMEA
                     I"
BASE COUNT         2539 a         6117 c         6009 g         2478 t
ORIGIN      
        1 gagcgccgcg ctcgtcctga cccagcccgt ggagcactcc ctcgccgctg cggcgaagcg
       61 cccggttccc cccgcgccgg aatcgccggc gatgacggcc gcgacccgga aggagatcgc
      121 gagccagaag aagtcggtgg ccgacatgct caaggtaatc cgtgcctaca agcttccgcc
      181 gggcagccgg cccgcgttcc tgttccgggc cgagaagcgg agcgtctccc gtgcctgacc
      241 tccacgccga tctctgtttc caatcggtcg cgcagctctc cgatctcatc cgccggcgca
      301 agctctctcc cgtcgagctg accgaggcct atctcgaccg gatccggcgc tacgcggcgg
      361 agatgaacgc tttcgtcacc gtgaccgacg atatcgcgat ggagcaggcg cgcgaggccg
      421 agcgcgagat ccagcgcggc cggcggcgcg gcccgctgca tggcattccc tacggcgcga
      481 aggatctcct cgccacggcg gggatcaaga ccacgtgggg ggccgcgccg acccggagcc
      541 agatgttcga ccgggacgcg accgtgatcc ggaagctgcg cgaggcgggc gcggtgctcc
      601 tcgggaagct ggggatggtg gagttcgcgg gatgcctcgg ctaccggttc gccaacgcgt
      661 cgctgaccgg cccgggacga aatccgtggg acctgaaccg ctggacggga ggatcctcgt
      721 cgggatcggg cgccgccgtg gcctcgggct tggtcggctt cgccatcggc accgagacat
      781 ggggctcgat cctctgccct tccgcgttct gcggcgtgac ggggcttcgc cccacctacg
      841 gccgggtgag ccgcgccgga ggcatggtcg gctcgttcac cctcgacaag attgggccgc
      901 tcgcgcgaag cgcgggcgac tgccgcctgg tgctggacgc catagcggga cccgatcccg
      961 acgatccgag cgcggccgac gagccagtcc gcctgcggcg gcgccggctg gacccgcgga
     1021 gcgtgcgcgc ggccctcgtg ccgctcgatt tcgcgaagac cgccggggcc gagcccgagg
     1081 tcggcgcggc gttccgcgcg gcggtggacg agctgcgcgg agcggggctc cggatcgagg
     1141 aggcgaagct gcccgagttc cccgcctcgg aggtcgcgac cacggtgctg tacgccgagg
     1201 cgctctccac gttcgagaag ttcttcgacg acggcacggt gcgccagctg accgacccgt
     1261 acgcgcccta ccagcgcgag gtcaccgagg ccctcacggg agccgactac gtgaaggcga
     1321 cgcgcatgcg aacgttactt caggaaaaga tgctcgactt ctactcgcgc tacgacgtca
     1381 tcgtcacgcc caacttcatg tcggtcgcgc cgcccgtggc ggaagacctg gtgaagaccc
     1441 tcccctacgc cgaccccgtc ggcgcgatcg ggaatgcgtg cggcctcccc gcgatcgcgc
     1501 tgcccgcggg cttcgggcgc gggcacatgc cggcctcgtt ccaggtcatg ggatcggcgt
     1561 tcgaggaagg attgctgctc gacctgggcg agttgtacca gtcgcgcacg tcgttccacg
     1621 aggcgcgccc accgaggtac gcgtagtcgc gtgaccccgc taccccgcta gcgcttcgct
     1681 cttttcccgg atcgcgtcga tcgcgcggtc cacgtgcgcc ttgtgcgtgc gcgaggccag
     1741 gatcgccaga cgcaggaagc gccgccccga gagccgcgtg gtcgagagga aggcgcggcc
     1801 gtcctcctcc agcgcgcggg ccagccggtc gttccgctcg tcggcgtcgg cgcggtcggg
     1861 cgccatccgg aagaccacga tcgagagatc gggctccggt cccacctcca gccccggaat
     1921 ctccaacagc cgccggtgcg cgtagcgcgc caggagcagc ttctcctcga gcgcggcctc
     1981 gaacggccgc gtcccgagga gcaggagcgg aagccagatg cgaagcccgc ggaacgggcg
     2041 gctcagctcg ggcgagagct ccgaggggga cggttcttcc acctcgtgcg cctcgtcggg
     2101 gacgtacgcg gcgtcgaagc ggtgcgccgc gcgcatcgcg cgcgcgtcct tcaccagcac
     2161 ggcgcccacg ccgtacggaa tatgcagccc cttgtgggga tcgagcacca ccgagtcgga
     2221 gcgctcgatg ccggacagga tccgccgccc ttccggggcc agcgcgaaca tgccgccgta
     2281 ggcgccgtcc acgtgcagcc agacgcgccg gcgccccgcc acgtcggcga tggcgtccag
     2341 cggatccacc gcgccggcgt ccgtggtgcc cgcgctcgcg acgatcagcc agggaatccg
     2401 tcccacccgg gcgtcctcgt cgatcatccg ctccagagcg gcgggatcca tgcgccatcc
     2461 cgcgtccatc gggacgcggc ggagcgggga ttccccgagc cccgcgatgc ggagcccctt
     2521 cggaacgcag tgatggacgt gttcggtggc gtagaccacg gcgcgcggca cctgctccga
     2581 gcggaggttc gccgcctcgc gcgcggcgag gatcgcgctc aggttggcga gactgccccc
     2641 cgaggcgagc gagccggagg cgttcgccgg atagccgacc gtggcgccga gccagcggag
     2701 cacctggttc tcgacgcgca ccgcgccggg acttcccagg aaatggccgg tgaagcggtt
     2761 gctcacggcg gcgaggaagt cggcgatggc ggcgtgatag aggtttccac ccgggatgta
     2821 gccgaagaag ccgggcgcgt gcggcgtcat gcccagggcg tccacgtgcc ggacgaggag
     2881 ggagaagacg gtcgcggcgt cgaccggctc ggggccgatc gggaactcgg tgatcgcgcg
     2941 gtcgccgtgg tcgcgcacgc tgggaacgcc ccggtcgact tcgtcggcga gcgcctcggc
     3001 gtggacgaac gcggcttcgg ccagggactc ccgctcttcc gcgtcggggt gaagcggggc
     3061 ggtcagccgt tccagctcga ggagccgggc gaggaggggg tcggccgcgg tggccgcggc
     3121 gccccggtcg tgggccacgc cggggactgt agcacgcggc tcagcccgga cccaggaacc
     3181 tgacgatctc ctgccgcagc cagggcggcg gataggcggc catgaaggcc atgacgccca
     3241 gcgtcgcgag caccaggagg acccccttga gcccgcggct ccgggagcgg cgctgcatgt
     3301 ccagctcgta gaaccgccag aggagctcgg ccttttcctc aggcgtcagc ggccgtccct
     3361 tggccagctt ctgcaccact ttcggcaggc gccggctgtc cccccggtag ctgccgttcc
     3421 actgcgggag cggcagatcc ggccgcccct cctggatttc ccatccccgt tcgtgcttgt
     3481 ccatggctcc cccgaacccg gttgcggagg cagtcccccg aatcccagga atcggcagcc
     3541 tgggcctccg gcttgaggag aaatcgtggc cgttggggcg gccggcggga cgcgcgcgga
     3601 agccgggtca ggatttcaga taccgctcgc gcaggacgtt ctggtggtgc agctcgtggc
     3661 ccacgatcac gtacgccgcc gcgcgaacgc tcatccgcgc gtcgttcgcc gtgccgcagc
     3721 gcccccacat cgcttcggga agattctcga agaaggagac cgtggcggcg cggacggcgc
     3781 gatgctcgtc cagcaggctc gacagcgtgc gcgacgcgta ccccgattgc ggcgtccacg
     3841 cgttctcgtc gaacccggga agcggcgtgg cgtcgttgcg ggagaagcgg agcgcgcggt
     3901 aggtgaacac tctctcgcag tcgatcacgt gccccagcac ttccttcacg ctccacttgt
     3961 cgggcgcgta gcggaagtcg ccgcgcgctt ccggcaggcc tcccaagagc ccgcgcgtgg
     4021 aatccacccc cgaccgcatc gcgccgagga ggtcgtcgcc gacgaggtcg atgtaacggc
     4081 cgtaatacgg gaggaattcg ccgggggccg gccgagcaat cagcagcata cgtggaggct
     4141 cctttcgtgt atggtcttcc gatgccattc gccacactgg gcttaccgcc cgccgtcctc
     4201 aaaggggtac gggccgccgg ttataccgat ccgacgccgg tccagaaccg cgccatcccg
     4261 ctcgtcttgt cgggacagga tgtgatcgcc gcggcgcaga ccggaacggg gaagacggcg
     4321 gcgttcatcc tccccatctt gagccgtctc atcgaggggc ccaagcgcat ccgcgcgctg
     4381 gtgttgacgc cgacgcgcga gctggccgcc caggtggaga cgaatgcccg cgattatgcc
     4441 cggttcaccg acgtccgggt gggggtcgtt tacgggggcg tgccgctccc gccccaggag
     4501 cgccagctga aggccggggt ggagctgctc gtggccacgc cgggccgcct cctcgacctg
     4561 cacgaccgcc tcgcgctgcg cctcgacttc atcgagatcc tggtgctgga cgaggccgac
     4621 cgcatggtgg acatgggctt cgcgcccgac ctgcggcgca tccttcgcct cctgccgacg
     4681 gagcgccaga cgctcctctt ctcggcgacg atgccgcccg aactgaattc ggtggccaag
     4741 gacgcgctca agaatccggc tcgggtcgac ctggccccgc cctcgcggcc ggcggccggg
     4801 atcacccagg cggtctaccc cgtgagccgc gacaagaaga ccgaccttct cgatgccatg
     4861 ctctccaagg acgaggcgtc cagcgtgatc gtcttcgcgc gcaccaagca cggcgccgac
     4921 cgcctcgcca agctgctcgt gcgccgcggc cactcggtgg cctcgatcca cgggaaccgg
     4981 agccagagcc agcgcgagcg cgccctgtcc gacctccgcc gcggacgcgt ggccgtgctg
     5041 gtcgccaccg acatcgcctc gcgcggcatc gacgtcgagg ggatcaccca cgtgatcaac
     5101 tacgacgtgc cgcacacgcc cgaggactac gtgcaccgca tcggccgtac cggccgcgtc
     5161 gacgccgtgg gcgacgcctt cacgctgatg gctcccgaag aagggaagca gatggccgcc
     5221 atcgagcgct tcctcggccg cgccatcccg cgcgtcacgc tgcccgactt cgactacaag
     5281 ggccgcccct cggagggacg cccccgcgag gagcatcgtc accgcgagga tcgggccgga
     5341 aacggcgcgc ctcgcgagca tggcgccggc ggccgggaac gccgcggcgc gagttaccct
     5401 gggtcctcgc gcgagagccg ccacagcggc ggcgcgaccg ccggcccgcg gcgcggcggc
     5461 ggcggcgggc gccgacccgg ctcgggcgca tccgcctccc ggtcgggcgg atcgcggaac
     5521 ggcggctccg gccgcggcgc gggccccgag agcgaccggc ggcgccccga ccatgcccct
     5581 cccgcgggag aggccgaacg ccacggacgt cgtccccgca aggtgacctc ggccgagcgc
     5641 atgcggcgag gacgccacta gcccgttcgt catgagcttc aagccggcat ccatcgagat
     5701 catcggcgcg cccgtggacc tgggcgcggg acgccgcggc gtggacatgg gcccctcggc
     5761 catccgcatc gccgacctcg agtcgcgcct cgaagcgctg ggacacagcg tctccgacca
     5821 cggcgacgtg gacgtgatga tccccgagac gctggaggtg ggcgccggag tcctccgcta
     5881 caaggcggcc atcctggccg cctgcgaatc gctccggatc gaggtggagc ggatccacgg
     5941 cgccgggcgc acgccgctcg tgctgggcgg cgaccactcg atcgccatcg gatcggtcgc
     6001 cgcgacctcg aaccatttcg acagccgctc ccagccggtc gggctgatct ggttcgacgc
     6061 ccacggcgac tgcaataccc cgctcacgac cccttcggga aacatccacg gcatgtcgct
     6121 cgcggtcctc ctgggtcagg gcgactcgga gctggtgcgc ctgggggggc gcgcgccgaa
     6181 agtgaagccg cagcacgccg tggtgatcgg cgtgcgcgac ctcgaccccg gcgagcggga
     6241 gatgctccgc gactccggcg tcgcggtctt cacgatccgc gacgtggacg agcgcggcat
     6301 gaagaccgtg gtgggcgagg cgatccggat cgccggctcg ggaacggggg gaatccatct
     6361 ctcgttcgac atggacgtgc tcgatcccga ggaggcgccc gggatcggca cgccggtgcc
     6421 cggcggcatc acctaccgcg aagcgcacct cgccatggag ctcctctgcg agagcgggct
     6481 cgtcgtggcc gccgatttcg tggaggtgaa cccggtgctc gacacccgga actcgaccgg
     6541 tgctctggcc gtcgagctgg cgctcagcct cttcggcaag cagatcctct agcgcctagc
     6601 gccgctccgc cagaagctgc agctgccaga ggtcccggcc gctcccccgc cagagcggca
     6661 gcatcgcgag cttccccgtc ggtccgatcg accgctcgag gaatttcatc tcgacgaacg
     6721 cggcgcgcac cgcgggatcc accgggagat cgatccccgc cgcgcgcgcg agcaggatcc
     6781 ctttcgcgcg gaggcccagc tccaggtgga tctggagcca gcagagcatg tcggccacca
     6841 ccggccccgg gaagcggagg cgcagcgatt cgaggtattc ccccgcgtgc gtatggcgga
     6901 actccccgct cgcgatcagc tccaggagct ccacctcgcc atccagtccc tctcccagcc
     6961 aggcctcggt cgcgcgctcg ctccgctcga agacgaacgc cacgaccagc ggcatcgtcg
     7021 cgaccaggag cccggccgag accagagggt tcagcaccac gtggttccac agggaatgca
     7081 gcacgatcgc cgcggccagc cccgggagga accaccggag cgagacgccg cgccgctccg
     7141 tgaggctttt cgccaggata cccgcgatcg cggtcgccga gccgtgcatc acggcggtgc
     7201 ccagcccgcg cgcgatccag acgccggcgc cggtggcgcc cgcgccggcg tagtaggcgt
     7261 tctcgaccag cgcgaacccg gcgccggcgg caaagccatg gatcgccgca tccaccagga
     7321 agccgatgcg cccgcggcgg atcagcacga cgagcagcgc cgccttgagc gactcctcga
     7381 cgaccggtgc ggcgtagcgg cgcaggagga ccggatcgag ccccgcgcgc aggagccccg
     7441 cgttgaccac gtaggcgatc ccggcggcga tcagtcccca gccgatcgag cgcagaacgg
     7501 cgcggcgcgc ggtcagcttg tagctgtcga ggaggaggag cgcggtaaga aagagcagga
     7561 ccgggaggac gcccgcgccc aggccgaaga gcgccgtcac cggatccttc tcacaggatc
     7621 ttcagcgaga ccatccactc gtcgtcgctg ggcccgccgc gcacgaacgc ccgcgcgtac
     7681 cctgccgaca gcgtgagcgg cgacacggtg aagagctgga gccggaggtc ggcctggatc
     7741 cccacgtccg cggccgtcgt gcgggaggag gcgtcgtcca ggttcgtcat gagccccccg
     7801 gcgaacagcg acgcgcggag ccaggtcgcg tagagcgcga gcgagcccgc ccgccggaag
     7861 cgcagcggcg ggaggttcag gtcgagcgtg gtcttcgcgt agttccgccc cgcgatcgcg
     7921 ttcaggtcgg cgcccgggaa cgcctcggcc tcccggtagc gcttggggtc gtggtagtcg
     7981 atcacgttgt tcccgaaccc gccgaaatag aagttcgcga acggctcgtc ccgatccccc
     8041 ggcgagagcc cggccgccga ccggagccag accgaggcgt gcggcgcggg aagcggcacc
     8101 cccagatcga gcgttccctc cgcgcgcggg aatccgcgcc aggcggcgtg tcccccgcgg
     8161 acgaaccgga cgccgtcggt cgcggccgtc agcgatccgc gccatccgcg ctcgtagtcg
     8221 accgagccga tcgagctgcg cacgttccgg aaccggagtc ccgcggagcc ggagaggagc
     8281 ttgtcgaatc ccggcgacgt cgagacgttc tggtagtccg gcaggcgctc cagcccgccc
     8341 cacccgctca cggccacgtc gagatccagg gacttcggcg cgtcctccat cagcgtgcgc
     8401 gcgtacccca gcgtgccgcc gtatcccttc cggctcacct tcgtcggccc gcccagatcg
     8461 tagaaggaag cgccgttcca gcgcgcctcc agcttccagg gggcgcgcgc ggcgcccagc
     8521 gccgcatgcc agcgctcgtc ggccggcagc ccgcgcgccg gcgcgtagga caacgagagg
     8581 tcgatcgcgt ccaggccgag cggatcggaa acgatggcct ggagtcccgc cgccgtgtag
     8641 tccttgtagc cctcgacgat cgggtagatc gaggccagcc ccagggaaga cgccgcgcgg
     8701 tagttcccct ccttgcggat gagcgagtcg atcggcacgg tcgacggagg cggcacgcgc
     8761 caggtcgtca cgagcgggcg ccgttccgtg agcgcctgcc ccaggaacgt gatcgcgctg
     8821 acgtcctgga gggggcgccc ctcgatcgcg gcggggacga accccccgcc ggcgtagcgg
     8881 aacacgatga gcgaatcgcc gccgagcgga atcgggcgga agaagccggt ctcggcattg
     8941 gtcaccacgc tcatcgaatc gccgtcgaag tcgtagcgga agatgttcga cacgcccgtg
     9001 tagtaggagc tgccgtacag gcgccggccg tccggagaaa agacgaaccc ggcggggatc
     9061 gacgcgccga agtcgtagag ctggaccgac gccgtgtcgc cggcgatcag cgaatcggtc
     9121 ttccagaggc ggagcgtctg ccgcccgctg atctcggcca cggagccggc gagcatcttc
     9181 ccgtcgggcg agatgtcgat gtcgtagaga tcgcggccga acggaaagac ggcgacctgg
     9241 gcgtagtcgg tgtacgggcg cagcatgcgc accagggtcg agacgccgtt gaagtgccgg
     9301 atcccccaga gggtgccctc cttcgcgtcc cacgccaggt ctcccacccg cgcgtccttc
     9361 tggaggacgc ggctcttcct cgtgcgcagg tcgtaggcgc agaggtcccg ccattcgttg
     9421 ttgtcggccg tgtagaagat cgtgcgggtc tcgggatcga acgccagtga ggagacgaag
     9481 tagagcgcgg gacccttcac ctcgagaagc cggcgcggcg gccccgacgg cggcggggct
     9541 tgcgtccccc cgccggcctc cgggccggcg cccgcgatgg ccgcgggggc gtcctcggcg
     9601 gcgggcaggg gcaccgccac gagcgacgcg ggcgcgcccg ggtagtacgc ggcggcgatc
     9661 agggagcgcg acgcggggtc gacgaaggcg cgcgagacgg agccgagcgg ccggtcggag
     9721 agatcgcgat accccgtgac cggatggagc cggatcgagt cgaggttcgc ggtctggaag
     9781 cgtcgctccc aggcgatcca tcgccgccac tcgtcgttca aggaccgccc gtacaccttg
     9841 tggaactgcc ccgcgaacca gcggtgcgat ccggggcggc gcgcgatcca cgtcatcagc
     9901 gattccgggc cgtactcgta cgcgaggtag gtcatgaacc gcgtgccgta caggtaggag
     9961 ttcacgccca cctggaagtc caccttcgcc ccttccgact ccagccccag cggatcgtag
    10021 aagcggcttc cgtccttggt catcgcgcgg aagaccatct cgtcgtacgg accctgcgcc
    10081 cgtcccagcc cgccggccat ccaggtctcc aggaagaccg cggccccttc gtggtaccag
    10141 cgcggcgccg cgcggcgcgg cgtcgtgagg aacgagtaga gcaccgactc ggggtggcgc
    10201 gacgtgtcgc gcaccttgcc ggcgaacacg ccgcggaaga agcggtccat ccccgccgcc
    10261 tggtccagcg cggcgacgtg gaccccctcg tggttcatcg tgaagttgat ccgctcgttc
    10321 gagggccccg tctcgtagac gaagttcacg ggcgcgatgt ggacgaccat gatgttccgc
    10381 ggcgtgaccc cgaccccggc gttcccgaaa tcggaagcgt cgtccaggat cacgttcacc
    10441 ttctcggtgg gccggtaatc gaagaggcgc tcgtggaagc ggatcgagtt ctcgaagcag
    10501 cgcgcggcgt agggggcgag gaaggagagg gcgggcgcgg gatagaccag cctcaggtcg
    10561 tcggtctcga ggaccttgag ctgggcccgc gcgggcgtgg gcccggccgc gaggagaagg
    10621 gccgcgatgg ccgcggccca ccaccgccgc gcgcttcgcg gacgcccatg cgggcaaacg
    10681 aacgaggagg ccccccccac gaggcctccc cgcttgatgc tctcgggcgt ttccagcccg
    10741 gcttgcgtca cgagattcgg gaaccctgct ttcttggacg gtctactggc cgctctgagc
    10801 cgactgcgac gcggtctttt ccagctcctg cggcgccggg acgctgaccg tcttgccgtt
    10861 ctcggtcggg accaggatcg tcttgccgtt ctcggtcggg accaggacga acttgccgtt
    10921 ctcggtcggg acccgcaccg tcttgccgtt ctccgtcggc accagcacgg ccttgccatt
    10981 ctcggtcggc accagcacca gcttgccgtt ctccgtcggc acccgcaccg tcttgccgtt
    11041 ctcggtcggc accaggatcg tcttgccgtt ctcggccggc accaggatcg tcttgccgtt
    11101 ctccgtcggc accaggatcg tcttgccgaa ctcggtcggc accagcaccg tcttgccgtt
    11161 ctcggtcggg acgaggatcg tcttgccgtt ctccgtcggg accaggatgg tcttgccgtt
    11221 ctcggtcggc accttcacgg tcttgccgtt ctcggccggc accaggatcg ccttgccgtt
    11281 ctcggtcggg accaggatga gcttgccgtt ctcggtcggc acccgcacgg tcttgccgtt
    11341 ctcaacggcc accaggaccg ccttgccgtt ctccgtcggg accagcgtca gcttcccgtt
    11401 ctcggtcggc accagcacca gcttgccgtt ctcggccggc accaggatgg tcttgccgaa
    11461 attcgggtcc gtcgagatct tgtggaacag gtcgctctgg aggaacgcct gcaccgcggg
    11521 atcggacaat cccacgtcct tgtccgagat ctgctccact tggtaccgct tggctccggc
    11581 gatcgttcct tccacgcccg agcgaggcgg gatcagtccg ttcatggccg cgatgcccac
    11641 cccagccgct accgcgagac ccgcgacgac gatccagcgt ttaccgttca taggacctcc
    11701 ctcctctcgc gaaccgccgt caccttgtcg cgccggccgt tcgcatgcaa gacacccatc
    11761 gcgccgaaaa gttggcgcgt gttgcgaaaa aagcggatca gttcgttttc ggccccgcca
    11821 ggccgtcggt aggaggggtc gcgctcgccc ggagcgcgcc tcgaaccgtc ccccccgaag
    11881 cttcgaccgc gatgcggatc ggggcgtcac cggcacccga gcccacgacg aggccgattt
    11941 cggcgcggtc ccgtccggac tgacgaatgc ggacccagcc gggtccatac tccaccccgc
    12001 cccgggaaga ggggacggtc acgaggcgct cgacccggga cgggcccggg gggagctgga
    12061 ccgtgaccag gaccgggtcg gcggcatcgg cctgcacggc caccaccgcc cgatcgtccg
    12121 accggaacac ttcgaaccgg acgttcgccc ggcccagctc gaagctctgg tcgtccacct
    12181 tggttccggc cttgccgccg gcgacgctgc cggttccccc gacgaggggc gccatcgcgc
    12241 cgtcggacgg catactgccg gttccccgcc acaggccact ccccgaagcc agggcgaaga
    12301 cgatcacgcc cgccgcggcg cccgccgcga acggaatcac tcgtgggaac agtcgttggc
    12361 ggaactccgt ccgaaaggtc tcgaaccagc cctggcgggc cggctccgga cgggtcccga
    12421 tcgcgcggat gacgttctgc ctgaggtccg ccggcggatc ctccaacgtg acacggtcca
    12481 ggatgcgggc gaccgcttcg tcgctttcgt tctcgccttc ggcgcgcggc ttcatgtcag
    12541 cgcgactcct ttccgttcca ggatcgaccc gagaagcctc cgcgccgtga acaggcgcga
    12601 tttcacggtc ttctcgggaa gctcgatcac ccggctcatc tcctgatagg agagctgcgc
    12661 gaagtgccgc agcaggatca cctgccggta gtcgggaggc agctcttcaa tcgcctcgtg
    12721 gatgcgagcg ctggtgtcgc gctcctccga ctcccgctcc ggcgacggct gcgccgccgg
    12781 cgtctcctcg tccagcgcct cgtggcgcgg acgtcgctgc accaggttca aggtctcgtt
    12841 ccacaagatc cggtagaccc agctgaagaa tttccggctc cggtcgaagg tcgcaagctt
    12901 ctggtacgcc ttgatgaaag cgacctgcgt gacatcctgc gcgtcctccc ggtttcccgt
    12961 catgcggagc gccacgttga acaggacccg ttgatgcgcg gcgaccagct ccccgtaggc
    13021 gtcggcgtct ccgtccagcg accgccggat cagttccgcc tcccgcgctc catccatacc
    13081 tacacggccc gaggctggaa gttggcgggg ttccagggcg agtcggctac tcaatgcgaa
    13141 gcacgcggtc gagtcccgcg tagccgaaga tgctgcggat ccgcggactt aggttggtga
    13201 gcaggaaggt gtggcccccc tggtggaggc gcttgtaggt ctcgaggatg acgccgatcc
    13261 cggcgctgga gatgtattcg agctcggaga aatcggccac ggaggcgccc tgcaggcggc
    13321 ccagcgcggc cagcgcggcg tccgcctggg cggcatccaa gcggcccacc agccggaccc
    13381 ggtcctcgcc ttcgaaccgg acctcgaaca tcaggtcgcg acccgcttac ggaccgtcac
    13441 cgttccggta tgcccgtcgt ggtggtactc cagccggtcg gccagccgct gcacgagatg
    13501 cagccccagc ccgccggccc ggcgctcttc cagcggagcg tcgggaagag cgcggggacg
    13561 cgtgacgtcc cagggcggcg cgccgacgtc gcgcagcacc aggacgaccg cgtccccgtc
    13621 gcgcgcgagg ccgatcgcga ccggctccgc gccgttgccg tacttcacca tgttcgtgaa
    13681 gagctcctcc gccatcaggt ccaggtcgaa ggcgtgcgcc gcatccagcc cctcggcccg
    13741 caggaactcg gcgatcatgc cgaagatccc gtccaaggac tcgaaggatc gcgggaaccg
    13801 ccgctcgtcc acgcgcacac gatagccccg cgcggggccg cggtctccct tatttcacga
    13861 ccgcgaggcg cccctcggtc gtcccttccg ccgcctcgat gcggtagaag tagacgcccg
    13921 aagcgagccg ccgccccgag gaatcctccc cccggatcgt gatctggtgc tcgcccgagg
    13981 ccaacgggcc ccgcggcagg agctcgcgca ccatgcgtcc cgacacgtcg tacagccgga
    14041 ccgataccgg gccggagcgc gacgtgacga agcgcagcac ggcttccgga ttcagcgggt
    14101 tcgggatcac cgtcgccgcg agcttgccgc cgcccgcgtt cacgtcgatg gacgccgtcc
    14161 cgcggaagcg tccgccggtg aggaggttcc cctggatcgc gaccggaacc gacacgctcc
    14221 cgcgaagcag gctgaagagc tgccgcaggt cggtcttggt gaagtacagc gtcatgtcct
    14281 cgatcttgtt gttgtcggcg tctccgaccg acggcgccgg cttggcggcg gtcccgatct
    14341 gcgagaccgt tcccgttccc tccgagatca ggacgatcga ggtgaggtcg acgtccatga
    14401 ggtcgaacga cccgtcgatc ggctcgatgg cgagctggta gtagggcttt ccggatccca
    14461 ggttgagctt ccgcgcgccg acggtgaaga cccgcgtcgc ggccgcgccg cgcacgtgga
    14521 tcaccgtgga ggcggacccg acgagccggt tggtagcctg gaacgtgacc gtgtagctcc
    14581 ccgagtcggc cagcgtcggc gtccaggcga gcgtccccag cgtgtgcgag gcgttggtcg
    14641 tgaaggtcgc gttgttcccg gaaggaagcg cgctccagtc ggccgtgagc gtgaggatcg
    14701 gatcggcgtc ggggtcgtcc gcgccgacgt tcaccgtcag cggcaggccc gcgtccccct
    14761 gcttcagagc cggggcgctc acgatcggat cgcgatccac gacgaattgc gcgtcgttcc
    14821 ccgtgggcag cccgctcaga tccgccgtga gggtgatgat gccctgctgc gccgcctgcg
    14881 acgaggccgt ggtggaggag gcgcgctgca gacgcccggc gaggagcgtg gtgcccgacg
    14941 tggccgtggt cgtcccggtg agcgtggtcc cggtgctcgt gctggtcgag ccggagtagc
    15001 tgatgatcgg gcccttcatc ggcgcctgcg gcgcgagggc gccgggggcc gccgccgcgc
    15061 gctggggtcc ggccagcgcc gcgcccgcag ggagcacggt cagcgacacg ggcaggctgg
    15121 acgttccgcc caggtcggtg gcgttgaagg tgacggtata gttcccttcc tcacccgcct
    15181 gcggcgtcca ggtgaagcgt cccgtgaccg acgtgccgag ggcggccacg aacatctccg
    15241 tggtggccga cgacgtggct ccgttcgcgg tcgcgctgaa ggtcaccgga tactcgcccg
    15301 cctgtcccag ccccggcgtc caggagagcg tgccggaggt gttgtccgcg ctggccgtga
    15361 aggaggccgc ggggccggcc ggaagcgccg agaggtcggc ggtgagcgcg tcgatcgcgt
    15421 tgccgtcggg atcggaggcg gacaccggca cgtcgatgtg gcccagctct tcgacggccg
    15481 cggacggcgg ggccgagacg accggaaccc ggtcggtgtc ggcgatcgtg atcgcggtgg
    15541 tcgcggagcc ggagagcgcg ttggtcgcgg tgaaggtcac ggcgtaggaa cccgcctggg
    15601 tgaagtccgg cgtccacgcg agcgtccccg aggtgttcga ggcattcgcg gtgaacgtcg
    15661 cgcccgccgg aaggggcgag gcggtgaagg aagcgatcgg ctggccgtcg ggatcggccg
    15721 cggtcgccgt gaccgtgagc gatgcggtct cggcaccgct cgccgtcgcg ggggcggtga
    15781 cgaccggcgc ggcatcgggg gtccgcgcga acgccacggc gtagagatca tccaccgcga
    15841 aggtctgcgg cgccgccccg cccgcctcga acccgagacg cgcgacgtcc gtcgccggct
    15901 gggcgaacgg gacgtcctgg aagccggcga tcgcgacgcc gtcgatcgag aggctcacgg
    15961 tgctcgtgag cagattcacg tcgaggcgga aggcgttgtt ccggttctgc tgatagacga
    16021 ccgggagcgt ccgcggcgca cccgcgatgc cgttccacgt gagggtgccg tgcgagccgt
    16081 attccaccga cgcgatcacg ttgccgttcg agccgcgcgc ggtgcaggcg acgatgttga
    16141 tgggattgtc gtcctgcgcg acggagcgcc agcccaggga aacccgctcg gtgccggcgg
    16201 gaggcgccgc cggccagccc cacaggctca cgccgcccac gccgctttgc tgcttcatct
    16261 gggcggccat ggtgagtccg tcgatggtcg cgttcacccg cacggtgccc gacgcctcgt
    16321 tcagcgtcag gaaatcaccg gccggcgcgc ctgggagcgc cagattcggc gcggccccga
    16381 ccacgtcggt cgagaagttc gcgaagagcg tgacggtcga catcgtgtcg ccgggcgcgg
    16441 ccgtggacgc aaaagccgcg gctcggatgc cgagcagcgc cagggaaagc aacaaggcaa
    16501 aacgtgagcg gaacatggcc agcctcgctt tcgggtgtcg tgcgcgggtt gtagtggaca
    16561 gtcgatggag ggtggtcctg ccggggagtg gccgcccttc ccccaagggc tgcacagggc
    16621 gtgaggatac gccgtcccag cggcaaattc aagagctcag atggcctcca tggccagctt
    16681 catggccggc tcgagggcga gcttgctgcc ctccgacagc tcggcggcga cccgctcccc
    16741 gagcgcctcc cgcgccgggg ccaggaaccg ctccatgcgg ctctcgtcgg gccccgagat
    16801 cggggtctgg atcgcctcgc ggatcgcccc cgccgcgccc agcaggcgga acgcgcgctc
    16861 cccccgccca tgggccgcgg cgagaaccgc gaacccctcc agggtcgagg cgattccctg
    16921 ccggtcctcc aggtcgcggt agatcgtgag ggcttcccga agacggtcgg ccgcggcgcc
    16981 caggtccccc cttcccgtcg cgacctcgcc cagctcgcgg agggagaagg cggtcccccg
    17041 ggtgtcgccc agctcgcgct gcacgggaag cgcctcctcg tggtagcggc ccgaggactc
    17101 caggtcgccg cgatagaacg ccacgccgcc cagcccgttc agg
//
LOCUS       DASSLZ010000265         5621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_478486, whole genome shotgun sequence.
ACCESSION   DASSLZ010000265 DASSLZ010000000
VERSION     DASSLZ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478486"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3136
                     /locus_tag="VFM00_10020"
     CDS             <1..3136
                     /locus_tag="VFM00_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HET7498625.1"
                     /translation="AADAIYFYGTGESGFGYDLKAGGGTEYQETQRTDLQPHWLTWGP
                     GPLATPPRRIAARDAAPVTAGAPLLTQVTHRVHFESNRFPNFDLFQPGLRWERWFMSV
                     LPQGARLAFPITLPGAVPGGEASARLRMWGQGTSTGHSARLWWDGAIVDTVSWNFLAA
                     RDLTASGLRTNGARDTVQAEVPAVFGRTDPEYLAWFEVSYPRALTASSDTIAFAAPDS
                     VAPGRYRYAIAGVSDTTAAWLLECTDPEAPVRLTGGIWSGASPAFTLTVEDSAGGGYR
                     PRYTLASTSRAPIPALARYAPAASPHAVADLLDAGNAADYLIVTPAAFLAEAESLAID
                     REALLPGVAAPRAAVATTEAIAAQLGGGRSDPVAIRNLFAYAERHWAAGPLYVCLFGD
                     ASDDPKNYQIISAADLVPTYNEYWDTSLLLQFISDDFYALLDGPGDRLVDLAIGRLPA
                     RTTEEARALARGKNAIYERTQDFDWWRARALLTADDAWKWSVPNTKRDPVGADHVTQM
                     ERKDGFHLPFPVRREKVYLNDYPFADSTKQSKPAAREAFLAAVNAGNWLVEFAGHGNE
                     NFLADEQMFRSLDVNRLTNAARPSIFGFFSCTVGKFDDNVQQDGLGELLLRHPGGGSV
                     VSLAASQEVFGLESGRLNDAFVDALFPAFPRVDTLRTAGLAWALAKNAPGNQNDVVRK
                     YGYLGDPAAIPPLPRGRGVFEKGALDSIPRGEVAAIRGHALNPDGSLDTLSTGTVLLE
                     ALGPPSRRIEIADRNGVGIAVPYALPGPVLYRGETALNQGTFTLRFVVPTDGRIVGPG
                     AQIRALLSQAGGRGVGLAADSLRIATSLAPRADATPPTIRLLGPAANDSTVAPGTVLT
                     FAIEDSSGIDLTRLDDAHSVFTIVDDRGTPIDMTPTFRYEPSSFTRGTATLTLPQLAG
                     GAHLLEVHASDTYRNVGIATFTIDVAAAVPPGSALVMTEVFNYPNPFPRETYLHARLN
                     RAARLRVQILTVSGRRVRDFALDGKAGENYIPWDGRDSEGEKVALGVYIVKLTAEVPG
                     GNRATAIARALRAE"
     gene            3248..4231
                     /locus_tag="VFM00_10025"
     CDS             3248..4231
                     /locus_tag="VFM00_10025"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HET7498626.1"
                     /translation="MSRFLTAASRAALALVLLAGFAASAQAQSEAGGISTLFAPGARA
                     DGMGRAFTAVANDANAIWWNPGGLAFIRGHDISLTYSQLVPDLASDVNWSYPVYAQHV
                     EGWGGLGASVGYLSYGKSEATDVDGNVTGEFTSYELVPSIAYGTELADNVGFGVALKL
                     IRVDLAPQAVTLDQKNGRGTTFAADIGGLIKMPAAKVAIGATLANLGPDIAYIDQDQS
                     DPLGRNIRLGLAYTPIENDVHRVLIAADASRFLIPGRVLAVDVWNAGAEYEFNRLIAL
                     RAGYISDPIGDITDFTFGLGLNYHGVRFDYASIPQSSFLDRVNRFSLGYHF"
     gene            4245..>5621
                     /locus_tag="VFM00_10030"
     CDS             4245..>5621
                     /locus_tag="VFM00_10030"
                     /inference="COORDINATES: protein motif:HMM:NF017368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A domain-containing protein"
                     /protein_id="HET7498627.1"
                     /translation="MTLRTGRRTARGAFLGIAVFMGSQALTSGLAPLAFAAGTPPSRL
                     VASRPPAGPAPGRDALLAKRIERAHSFPHRLGGPRRASWVSGGRPPLLAPSLRTGQLE
                     RTVAFAGAPETLRVLGLRVDFATDRLGSQTTTPDGKFDLRDGVSLGIPIDPPPHNRAF
                     FLSHLQALQRYWRFVSYGNLVLEYDVYPKNDTLTYRLGDTGDYGPWTLGQASFASAQR
                     FFKDAVTKADQTDSIPFGDFDVIALFHAGADFQTDLRGDSPRDFPTFQISLEDSVAVN
                     GGAVAVFGGLVMPETENQDGYYSALNGTLAHEFGHTQGLPDLYDINTFFPAVGVWSNM
                     DSGYLLTTSVQDQKTGAISDATGVIPTSLDPWCKNVLWPGKLDLVDPGRDYSASLRAT
                     ELHDSLLYVPLGGDEYYLIENRETDLNGDNTVYLDRDSTTNVILGPGLSSADPTDSLG
                     DREYDFLLP"
BASE COUNT          837 a         2061 c         1848 g          875 t
ORIGIN      
        1 cgcggccgac gccatctact tctacggcac cggggagagc ggcttcggct acgacctgaa
       61 ggcggggggc gggacggagt accaggaaac gcagcggacc gacctgcagc cgcactggct
      121 cacgtgggga ccgggcccgc tggcgacgcc tccgcggcgg atcgcggcgc gcgacgccgc
      181 gcccgtgacg gccggggcgc cgcttctcac gcaggtgacc catcgggtcc atttcgagag
      241 caatcgattc cccaacttcg acctcttcca gcccgggctt cgatgggagc gctggttcat
      301 gagcgtgctg ccgcaggggg cacggctcgc gtttccgatc acccttccgg gagccgtccc
      361 ggggggcgag gcctcggcga ggctccggat gtgggggcag ggcacgtcga ccgggcactc
      421 cgcgcgcctc tggtgggacg gcgcgatcgt ggacacggtg agctggaatt ttctcgcggc
      481 gcgcgacctg accgcgtcgg ggcttcgcac gaacggggcg cgcgacacgg tgcaggccga
      541 ggtgccggcg gttttcggac ggaccgatcc cgaatatctc gcctggttcg aggtctccta
      601 cccgcgggcg ctcaccgcct cgtcggacac catcgctttc gccgcgcccg actcggtcgc
      661 gcccggacgc taccgctacg ccatcgccgg ggtgagcgac accacggcgg cgtggctcct
      721 ggaatgcacc gatcccgaag cgcccgtcag gctcaccggg ggaatctggt ccggcgcctc
      781 gcccgcgttc acgctgacgg tcgaggacag cgcgggcggc ggctaccggc cgcgctacac
      841 gctcgcctcg acgtcgaggg cgccgattcc cgcgctggcc cgctacgcgc ccgccgcgag
      901 tccgcacgcc gtggcggacc tcctcgatgc ggggaacgcg gccgactacc tgatcgtcac
      961 gccggccgcg ttcctcgccg aggcggagtc gctcgccatc gaccgcgagg cgctgctccc
     1021 gggagtcgcc gctccgcgcg cggcggtcgc caccaccgag gcgatcgcgg cccagctcgg
     1081 cggcggccgg tcggacccgg tcgcgatccg gaacctcttc gcctacgccg agcggcactg
     1141 ggccgccgga ccactctatg tctgcctctt cggagacgcc tcggacgatc cgaagaacta
     1201 tcagatcatc tcggcggccg atctcgtgcc gacctacaac gagtactggg acaccagcct
     1261 tctcctccag ttcatctcgg acgacttcta cgctctcctc gacgggcccg gcgaccggct
     1321 cgtcgacctc gcgatcgggc gcctgcccgc gcgcacgacc gaagaggcgc gcgcgctggc
     1381 gcgggggaag aacgcgatct acgaacgcac ccaggacttc gactggtggc gcgcgcgcgc
     1441 gctgctgacc gccgacgacg cctggaagtg gtcggttccg aacacgaagc gcgaccccgt
     1501 cggcgccgac cacgtgaccc agatggagcg gaaggacgga ttccatcttc cgtttcccgt
     1561 gcgccgggag aaggtctacc tgaacgacta ccccttcgcc gattccacga agcagtcgaa
     1621 gccggcggcc cgggaggcct tcctggccgc ggtgaacgcg ggcaactggc tcgtcgagtt
     1681 cgcgggacac ggcaacgaga acttcctcgc cgacgagcag atgttccgct ccctcgacgt
     1741 gaaccgcctc acgaacgccg cgcggccttc gatcttcggc ttcttctcct gcacggtggg
     1801 gaagttcgac gacaacgtgc agcaggacgg gctgggcgag ctcctgctcc gccaccccgg
     1861 cggcggatcg gtggtctccc tggcggcgag ccaggaggtg ttcgggctgg agagcgggag
     1921 gctgaacgac gccttcgtcg acgcgctctt cccggctttc cctcgcgtgg acacgcttcg
     1981 gaccgcgggt ctcgcgtggg ccctggccaa gaacgcgccg ggaaatcaga acgacgtcgt
     2041 gcggaagtac ggctacctgg gcgatcccgc ggccatcccg ccgctcccgc gcggacgcgg
     2101 cgtattcgag aagggcgcgc tcgattcgat tccgcgcggc gaggtcgccg cgatccgggg
     2161 ccacgcgctg aatccggacg gatccctcga cacgctctcg acgggcacgg tcctgctgga
     2221 ggcgctcggc ccgccctcac ggaggatcga gatcgccgac cggaacggag tcgggatcgc
     2281 ggtgccctac gcgctcccgg ggccggtgct ctaccggggc gagaccgccc tgaaccaggg
     2341 cacgttcacg cttcgcttcg tcgtgccgac cgacggccgg atcgtcggcc ccggcgccca
     2401 gatccgggcc ctcctgtcgc aggcgggcgg ccgcggggtg gggctcgcgg ccgactcgct
     2461 ccgcatcgcg acctcgctgg ctccgcgcgc ggacgcgacg cccccgacca tccgcctgct
     2521 ggggccggcg gcgaacgact ccaccgtggc gcccggaacg gtcctgacct tcgcgatcga
     2581 ggattcgagc gggatcgatc tcacgcgtct ggacgacgcg cacagcgtct tcacgatcgt
     2641 ggacgaccgc ggcacgccca tcgacatgac cccgaccttc cgctacgagc cctccagctt
     2701 cacgcgcggg acggcgacgc tcacgctgcc ccagctggcc gggggcgccc acctgctcga
     2761 agtgcacgct tccgacacct accggaacgt ggggatcgcc accttcacga tcgacgtcgc
     2821 cgcggccgtc cctcccggat cggccctggt catgaccgag gtgttcaact atcccaatcc
     2881 cttcccccgg gagacctatc tgcacgcgcg gctgaaccgg gcggcgcggc tccgggtgca
     2941 gatcctgacc gtttcggggc gccgggtgcg cgatttcgcg ctcgacggca aggccgggga
     3001 gaactacatc ccctgggacg gccgcgattc agagggagaa aaagtggccc ttggggttta
     3061 tatcgtgaag ctgacagcag aggtaccggg ggggaatcgc gccacggcca tcgcgcgagc
     3121 gctccgtgcc gagtgagcat ccaccggcgg aaccgcttgt taccatgtcc catcgtgttc
     3181 tagacttagg gtctcgtcgg gggcgcgatc ggcgtccctt cctgcattcc gtaaggaggt
     3241 cttccacatg agtcgcttcc tcaccgccgc ctcgagggct gccttggcgc tcgtcctcct
     3301 ggccggcttc gccgcgtccg cccaggccca gtccgaagcc gggggcatct ccacgctctt
     3361 cgcgccggga gcccgcgccg acggcatggg tcgcgccttc accgccgtcg ccaacgacgc
     3421 gaacgcgatc tggtggaatc ccggcggcct ggccttcatc cgcggccacg acatcagcct
     3481 gacctactcc cagctggtcc ccgatctcgc ctccgacgtg aactggtcct atcccgtcta
     3541 tgcccagcat gtcgaggggt ggggcggcct gggcgcgagc gtcggctacc tgagctacgg
     3601 caagagcgaa gcgaccgacg tggacggcaa cgtgacgggc gagttcacct cgtacgagct
     3661 ggtgccctcc atcgcctacg gcaccgagct ggcggacaac gtcggcttcg gcgtcgccct
     3721 gaagctgatc cgcgtcgact tggcccccca ggcggtcacc ctcgaccaga agaacgggcg
     3781 cgggacgacg ttcgccgccg acatcggcgg tttgatcaag atgcccgcgg ccaaggtcgc
     3841 catcggcgcc accctcgcca acctgggccc ggacatcgcc tacatcgacc aggatcagtc
     3901 ggacccgctg ggccggaaca tccggctcgg cctcgcctac acgccgatcg agaacgacgt
     3961 gcaccgcgtc ctgatcgcgg ccgacgcctc gcgcttcctc atccccggcc gcgtgctcgc
     4021 cgtggacgtc tggaacgccg gcgccgagta cgagttcaac cggctcatcg cgctgcgcgc
     4081 gggctacatc agcgacccga tcggcgacat cacggacttc acgttcggcc tgggattgaa
     4141 ctatcacggc gtgcgcttcg actacgcctc gatcccgcag tcgagcttcc tggatcgcgt
     4201 caaccggttc tcgctcggat accacttcta agaacccttc gaacgtgacc ctgcggacgg
     4261 ggcggcggac cgcccgcggc gcgtttctcg gaatcgcagt cttcatgggg agccaggccc
     4321 tgacctcggg cctggctccc ttggcattcg cggccggcac gcccccgtcc cgcctggtgg
     4381 cgtcgcggcc cccggccggc cccgcgcccg gcagggacgc cctcctcgcc aagcggatcg
     4441 aacgggcgca ctctttcccg catcgcctgg gcggtccgcg gcgcgcctcc tgggtgagcg
     4501 gcggccgtcc accactcctc gcgccctctc tgcggacggg gcagctggag cgcacggtag
     4561 ctttcgcggg cgctccggag acgctccgcg tgctcggcct gcgcgtggat ttcgcgacag
     4621 accgcctggg atcgcagacc acgacgccgg acgggaagtt cgatcttcgc gatggcgtct
     4681 cgctcgggat cccgatcgat ccgccgccgc acaaccgcgc cttcttcctc tcccacctcc
     4741 aggcgctcca gcgctactgg cggttcgtct cctacggcaa tctcgtcctc gagtacgacg
     4801 tctacccgaa gaacgacacc ctgacctacc gtctgggcga caccggggat tacggaccct
     4861 ggacgctggg gcaggcgtcg ttcgcgtcgg cgcagcgctt cttcaaggat gcagtgacga
     4921 aggccgatca gaccgactcg atccccttcg gtgacttcga cgtcatcgcc ctgttccacg
     4981 ccggagccga ctttcagacc gacctccgcg gggattcgcc ccgcgacttt ccgacgttcc
     5041 agatctcgct ggaggactcg gtcgcggtca acggcggggc ggtcgcggtc ttcggcggtc
     5101 tcgtcatgcc cgagaccgag aatcaagatg gatactacag cgccctgaac ggaaccctgg
     5161 cccatgagtt cgggcacacc cagggccttc ccgacctcta cgacatcaac acgtttttcc
     5221 cggcggtggg cgtctggagc aacatggact cggggtacct gctgaccacg tcagtccagg
     5281 accagaagac gggggccatc tccgacgcga cgggggtcat tcccacgagt ctcgatccgt
     5341 ggtgcaagaa cgtcctctgg cccgggaagc tcgacctcgt ggatcccgga cgcgactact
     5401 cggcctcgct ccgcgcgacg gagctgcacg acagcctgct ctacgtcccg ctcggtggcg
     5461 acgagtacta cctgatcgag aaccgcgaga ccgacctgaa cggggacaac accgtctacc
     5521 tggatcgcga ctccacgacg aacgtgatcc tgggaccggg gctatcgagc gccgatccca
     5581 ccgattcgct cggcgaccgg gagtacgact tcctcctgcc g
//
LOCUS       DASSLZ010000266         8322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_478500, whole genome shotgun sequence.
ACCESSION   DASSLZ010000266 DASSLZ010000000
VERSION     DASSLZ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478500"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /gene="ruvX"
                     /locus_tag="VFM00_10035"
     CDS             complement(<1..466)
                     /gene="ruvX"
                     /locus_tag="VFM00_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET7498628.1"
                     /translation="MRVLGVDFGPRRIGLALSDEEARLASPLRAIAISSVREAPGAVA
                     SAAREADAGAIVVGAPLGLEGEEERPETRRVRRFAQALRRATGLPVHLVDESLSSREA
                     AERASAAGRDAATRARSGRGAAKDALHAAAAAVILQRWLDQAPLREAARQARA"
     gene            complement(454..1398)
                     /locus_tag="VFM00_10040"
     CDS             complement(454..1398)
                     /locus_tag="VFM00_10040"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7498629.1"
                     /translation="MPASRAGERLDRYLAEVERSLSRSAVQHLIDAGHVLVNDGPARA
                     SRRVKPGDRVLVTRPRRAPSVLAPEPIPLDVVYEDDALAVVNKPAGMVVHPAVGHRTG
                     TLVHALLHRYTALSSANGAERAGIVHRLDKGTSGLLLIARTEESHRELARQIEAREVK
                     RLYRAIVWGHPRGQEGRIEASLARSRSDRKKIAVVTRGGRSAATRWRVERRFDDLAEL
                     TLSLETGRTHQIRVHLAHLGHPVFGDAEYGGRRGPLLRLAPRRRAQVALILGELDHQA
                     LHAETLAFRHPVTRLPMEFTRPVPPDFAAALEALACAS"
     gene            complement(1376..1888)
                     /gene="lspA"
                     /locus_tag="VFM00_10045"
     CDS             complement(1376..1888)
                     /gene="lspA"
                     /locus_tag="VFM00_10045"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET7498630.1"
                     /translation="MTRRTGVFLACAAAVVGLDQVAKQAASSRLETGESVSVLGDFIR
                     LTLVHNTGAAFGLFPGSRVPFIVVSVVAIAVVLWLFLRETYRSVMNRVLLGCILGGAI
                     GNLVDRVRLGWVVDFIDMGIGTARWPVFNVADSAVTLGVLLLAWNLARSGRVAAPEPE
                     DPSRAGVPGG"
     gene            complement(1885..2274)
                     /locus_tag="VFM00_10050"
     CDS             complement(1885..2274)
                     /locus_tag="VFM00_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET7498631.1"
                     /translation="MLNKKDLKHFEDRLLEERKKLLGQLGYLERTLNQTQRDSAGDLS
                     AYSFHMADMGTDAMEREKTFLFASAEGRLLYNVDEALRRLYRNEYGICQSCGKEIGKA
                     RLDAIPQASLCVACQEKQEKETNTARG"
     gene            complement(2277..5453)
                     /gene="ileS"
                     /locus_tag="VFM00_10055"
     CDS             complement(2277..5453)
                     /gene="ileS"
                     /locus_tag="VFM00_10055"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET7498632.1"
                     /translation="MPAAAERFRPLSTRLDPAESEGRVLALWKEIRAFEDGLKRREGQ
                     PPFIFYEGPPTANGLPGVHHVISRTLKDIVCRYQSMTGHYVPRKGGWDTHGLPVEIEV
                     ERELKISGKEQIETFGIAEFNARCRASVLRYEAEWRRNTERIGYWLDMDHPYVTFHNE
                     YIESVWWLLQQIWDANLLYQGHKIVPYCPRCGTPLSSHEVAQGFDDVTEPSVTVKFAL
                     VDEPDTYVLAWTTTPWTLPGNVALAVNPDIAYVLVRQERDGRAERYYLAESRLEQLEG
                     AYAVERRLRGHELQGKRYRPLFDFIDLAKLTGKDAYYVAEAPFVTTEDGTGVVHTAVM
                     YGEDDYQLGLKLELPQHHTVDPQGRFTAEVTPWAGRFVKDAEAEIRAWLSSHGLLYRE
                     EMTTHAYPFCWRCDSPLLYYAWKTWYIATTRLREKMLEAHRTVAWHPEEIGRNRFGNW
                     IENNVDWALSRNRFWGTPLPIWRCEGCGSDRCVGSVAELRQGKGLPEPLDLHRPYVDH
                     VTFACSGCGGVMRRVPEVIDVWFDSGSMPFAQWHYPFENKEAFRVSFPADFISEGVDQ
                     TRGWFYTLLAISVAVTGKAPYRHVLVNELVLDKLGKKMSKSRGNAVDPNEVLELRGAD
                     ALRFYLIANSQPWSSTRFDVEGVTETAKKLLGTLRNTAQFFVLYANIDGFDPFAKEPV
                     SPSAMDRWILSRLQATILACRASLDGYELTRGARAIQEFVVEELSNWYVRRSRRRFWK
                     SGDPADKRAAYETLFTCLETVSRLLAPYAPFITEELHQHLVRPADPEAPKSVHWTDYP
                     VADAALVDETLERAMSLAERVVVLGRAARNQASLRVRQPLRRIAVAGLADGEQKALLS
                     LADVIQEELNVKELAFAKDRSELLTTAVKPNFPVLGKKAGPAMKSLASAIQAAPIDAA
                     RKALSAEGWEIEAEGQVFRLTAEDVQVQESPRAPWAAQSEGSLTVAVDTTLDDALRDE
                     GLVRELAHRVQALRKSADFEVTDRIRLYGELSPGLKRAFERHEETFKDEVLAVELVPG
                     AAGGEAAESWTFDGEQARVGIERVPKGG"
     gene            complement(5453..6355)
                     /locus_tag="VFM00_10060"
     CDS             complement(5453..6355)
                     /locus_tag="VFM00_10060"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET7498633.1"
                     /translation="MAQAGSTVVSAAPKGADDLPRRIEEARAFVASRSAVRPEVAVIL
                     GTGLGRFAEAMPGADVIPFEEIPGFPTTAVESHAGDLVLGTLGGKPTAVLNGRAHYYE
                     GWTMDQVVFPVRVTRALGARTLIATNAAGGMNPLYRPADIVAIVDHINLMGDNPLIGR
                     NHDSLGPRFPDMSEPYDRSLIALAHEVALEERIALQSGVLVGVAGPNLETRAEYRFLR
                     WAGADLVSMSLVPEAIAAVHGGMRVLAFAVVTDLCLPDALEAVDIPKILANAAKAEPL
                     LTRLVTRVLERMPTAASDAENPER"
     gene            complement(6328..6606)
                     /locus_tag="VFM00_10065"
     CDS             complement(6328..6606)
                     /locus_tag="VFM00_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HET7498634.1"
                     /translation="MPEAVRLQVTVAPKSRADEIQASRAEKTVRIRVTAAPEDGRANR
                     AVLDLLRERLGLPRSAVRIAGGEASRRKWIEIDGMDEEEVWRRLEAPS"
     gene            complement(6599..7354)
                     /locus_tag="VFM00_10070"
     CDS             complement(6599..7354)
                     /locus_tag="VFM00_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET7498635.1"
                     /translation="MFPSAAHLYTVRLTFSGGAGTRDLASLSNRLDDVRERIARAAER
                     SGRPAGSVRLLAVSKGIDAERVREAFRLGAVELGESRVQEAEPKIGLVGPGPRWHLVG
                     HLQTNKAKRAALLFDAIHSIDSERLAEEIGRRAAEAGRALEAYVEVNTSGDPGKHGVA
                     PEGAPALIERVAAIRSLRAAGLMTIGPLEGGEAGARVSFRALRRIRDEAVARGALAPD
                     AGLSMGMTDDFEAAIEEGATIVRVGSAIFGPDA"
     gene            complement(7417..7623)
                     /locus_tag="VFM00_10075"
     CDS             complement(7417..7623)
                     /locus_tag="VFM00_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498636.1"
                     /translation="MDTEYWYRCNRCGYMYTPQQVQALIQLHVSRSGAVPSDPNAIPS
                     RIPCRKRGCEGSVLRTTSVFREGR"
     gene            7849..8202
                     /locus_tag="VFM00_10080"
     CDS             7849..8202
                     /locus_tag="VFM00_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017314111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET7498637.1"
                     /translation="MGKILLHFVIIVLTFLLLANVVPGFAVKSWQAAVVAALVFGLVN
                     AILGPVLTLVSLPLIILTLGLFWFVVNAFLLILVGFIVPGFDFNGFLPALIVSVVLAA
                     VNLVWKAASRERHRS"
BASE COUNT         1202 a         2992 c         2871 g         1257 t
ORIGIN      
        1 ccgcgcgcgc ctggcgggcg gcctcccgga gcggcgcctg atccagccag cgctggagga
       61 tgacggctgc ggcggcggcg tggagcgcgt ccttcgccgc gccccgcccg gaacgagcgc
      121 gggtcgcggc gtcgcgtccc gcggcggagg cgcgctcggc ggcttcgcgg ctgctcagac
      181 tctcgtccac caggtggacc ggaagcccgg tcgcgcgccg gagcgcctgc gcgaagcggc
      241 ggacgcggcg cgtctcggga cgctcctcct ccccttccag gcccagcggc gctcccacca
      301 cgatcgcgcc ggcatcggcc tcgcgcgccg cggaggcgac ggcgccgggc gcctcgcgca
      361 ccgaggagat cgcgatcgcg cggagcgggc tggcgagccg cgcctcttcg tcggaaagcg
      421 cgagaccgat ccgccgcggg ccgaaatcca cgcctaggac gcgcatgcga gcgcttcgag
      481 cgcggccgcg aagtcggggg gaacgggacg cgtgaactcc atcggaagcc gcgtcacggg
      541 gtggcggaac gcgagcgtct cggcatgcag cgcctggtgg tccagctcgc ccaggatcag
      601 cgcgacctgc gcgcgtcgtc gcggcgccag gcgcaggagc ggcccgcggc ggccgccgta
      661 ctcggcgtcc ccgaaaaccg gatgccccag gtgcgcgaga tggacccgga tctggtgcgt
      721 gcgccccgtc tccagggaca gcgtgagctc ggccagatcg tcgaaccgcc gctccacccg
      781 ccagcgcgtc gccgcggagc gcccgccccg cgtcaccacc gcgatcttct tccggtcgct
      841 ccggcttcgg gccagcgagg cctcgatccg tccctcctgc ccgcgcggat gcccccagac
      901 gatcgcgcgg tagaggcgtt tcacctcgcg ggcctcgatc tggcgcgcca gctcgcggtg
      961 cgactcctcg gtgcgcgcga tgagcaggag gcccgacgtg cccttgtcca ggcggtgcac
     1021 gatccccgcg cgctcggcgc cgttcgccga ggagagcgcc gtgtagcgat ggaggagggc
     1081 gtgaaccagg gtgccggtgc ggtgcccgac tgcggggtgg acgaccatgc ccgcgggctt
     1141 gttcaccacg gcgagggcgt cgtcttcgta gacgacatcg agcgggatcg gctcgggggc
     1201 aaggacgctg ggcgcgcggc gcgggcgggt cacgagcacg cggtcgcccg gcttcacgcg
     1261 ccggctcgcg cgcgccggcc cgtcgttgac gaggacgtga ccggcgtcga tcaagtgctg
     1321 cacggcggaa cgggagagac tgcgctcgac ttcggccagg tagcggtcga gccgctcacc
     1381 cgcccgggac gccggcacga gatgggtctt cgggctctgg ggcggcgacg cgcccggatc
     1441 gcgcgagatt ccaggcaagc aggagcactc ccaaggtgac ggcgctgtcg gccacgttga
     1501 agaccggcca gcgggccgtt ccgataccca tgtcgatgaa gtcgaccacc cagccgaggc
     1561 gcacgcgatc caccaagttg ccgatcgcgc cccccaggat acagccgagc agcacgcggt
     1621 tcatgacgct ccggtaggtc tcgcgcagga acagccagag cacgacggcg atcgcgacca
     1681 ccgagacgac gatgaacggc acgcggctgc ccgggaagag cccgaacgcc gcgcccgtat
     1741 tgtgcaccaa agtgaggcgg atgaaatccc cgagaacgga gacggactct ccggtctcca
     1801 gccgcgagga ggcggcctgc ttcgcgacct ggtccaatcc gaccacggcc gccgcgcagg
     1861 cgaggaagac cccggtgcga cgcgtcatcc gcgggccgtg ttcgtctctt tctcctgctt
     1921 ctcctggcat gccacgcaga ggctggcctg cggaatggcg tcgaggcgcg ccttgccgat
     1981 ctccttgccg cacgactggc agatgccgta ctcgttgcgg tacaggcggc ggagtgcctc
     2041 gtccacgttg tacagcaggc ggccttcggc cgaggcgaag aggaacgtct tctcgcgctc
     2101 catcgcgtcg gtgcccatgt ccgccatgtg gaacgagtag gcggagagat cgcccgccga
     2161 atcgcgctgc gtctggttca acgtgcgctc cagatagccg agctgtccca gcagcttctt
     2221 ccgctcctcg agaagccggt cctcgaaatg cttcagatcc ttcttgttga gcaacgtcag
     2281 cctcctttgg gtacccgctc gatcccgacg cgggcctgtt ctccgtcgaa tgtccagctc
     2341 tccgccgctt cgccgcccgc ggcgccgggc accagctcga cggcgaggac ctcgtccttg
     2401 aaggtctcct cgtgccgctc gaaggcccgt ttcaatcccg gcgacagctc cccgtacagg
     2461 cggatccggt ccgtcacttc gaagtcggcg ctcttccgga gcgcctgcac gcgatgcgcc
     2521 agctcgcgca ccagcccctc gtcgcgaaga gcgtcgtcga gcgtggtgtc gaccgcgacc
     2581 gtcagcgagc cctccgactg cgccgcccac ggcgcccggg gggactcctg cacctgcacg
     2641 tcctccgccg tcaggcggaa gacctggccc tcggcctcga tctcccaccc ctccgcgctc
     2701 agcgccttcc gggccgcgtc gatcggagcg gcctggatcg cggaggccag cgacttcatg
     2761 gccgggcccg ctttcttacc cagcacgggg aagttcggtt tcaccgccgt cgtgaggagc
     2821 tcgctccggt ccttcgcgaa ggcgagctct ttcacgttga gctcctcttg gatgacgtcc
     2881 gccagcgaga ggagcgcctt ttgttcgcca tcggcgaggc cggccacggc gatccgccgg
     2941 agcggctggc gcacccgcag cgaagcctga ttccgcgcgg cgcgcccgag cacgaccacg
     3001 cgctccgcga gcgacatcgc ccgctcgagc gtctcgtcca cgagggccgc gtccgcgacc
     3061 gggtaatcgg tccagtggac gctcttcggc gcctccgggt ccgccgggcg gaccaggtgc
     3121 tggtgcagct cctccgtgat gaagggagcg tagggcgcga gcagccgcga caccgtctcg
     3181 aggcaggtga agagcgtctc gtaggccgcg cgcttgtcgg cgggatcccc ggacttccag
     3241 aagcgccggc gcgagcggcg cacgtaccag ttcgagagct cctcgacgac gaactcctgg
     3301 atcgcgcgcg cgccgcgcgt cagctcgtag ccgtccagcg aggcgcggca ggcgaggatc
     3361 gtcgcctgga ggcgcgagag gatccagcgg tccatggccg agggagacac cggctctttg
     3421 gcgaacggat cgaagccgtc gatgttcgcg tagagcacga agaactgggc ggtgttccgg
     3481 agcgttccca acagcttctt cgccgtctcg gtcacgccct ccacgtcgaa gcgcgtcgag
     3541 ctccagggct ggctgttcgc gatgagatag aagcgaaggg cgtcggcgcc gcgcagctcg
     3601 aggacctcgt tgggatcgac ggcgttcccc cgcgacttgc tcatcttctt gcccagcttg
     3661 tccagcacca gctcgttcac gagcacgtgc cggtaggggg ccttccccgt gacggccacg
     3721 ctgatcgcga gcagcgtgta gaaccagccg cgcgtctggt cgacgccctc cgagatgaag
     3781 tcggccggga aggagacccg gaacgcctcc ttgttctcga acgggtagtg ccactgcgcg
     3841 aacggcatcg agccggagtc gaaccagacg tcgatcacct ccgggacgcg tcgcatcacg
     3901 ccgccgcagc cggagcaggc gaaggtcacg tgatccacgt acgggcggtg gagatccagc
     3961 ggctccggga gccccttccc ctggcgcagc tcggccacgc tgccgacgca gcggtcggag
     4021 ccgcatccct cgcagcgcca gatggggagc ggcgtccccc agaagcggtt ccgcgagagc
     4081 gcccagtcca cgttgttctc gatccagttc ccgaatcggt tccggccgat ttcctccggg
     4141 tgccacgcca ccgtgcgatg cgcctccagc atcttctcgc gcagccgcgt ggtcgcgatg
     4201 taccaggtct tccaggcgta gtagaggagc ggcgagtcgc agcgccagca gaagggatag
     4261 gcgtgggtcg tcatctcctc gcgatagagc agcccgtgcg acgagagcca cgcgcggatc
     4321 tcggcctcgg cgtccttgac gaaccgcccg gcccaaggcg tcacctcggc ggtgaaacgg
     4381 ccctgcggat ccaccgtgtg gtgctgcggc agctccaact tcagaccgag ctggtagtcg
     4441 tcctcgccgt acatcaccgc ggtgtgcacg acgccggttc cgtcctcggt ggtgacgaac
     4501 ggggcctcgg ccacgtagta ggcgtccttg cccgtgagct tcgccaggtc gatgaaatcg
     4561 aagagcggcc ggtagcgctt cccctggagc tcgtgccccc ggaggcgtcg ctcgacggcg
     4621 tacgcgccct cgagctgctc cagccgggac tcggccaggt agtagcgttc cgcgcgcccg
     4681 tcccgctcct gccggaccag cacgtaggcg atgtcggggt tcaccgcgag cgccacgttc
     4741 cccggcagcg tccacggcgt cgtggtccac gcgagcacgt acgtgtcggg ctcgtccacc
     4801 agggcgaact tcaccgttac cgacggctcg gtgacgtcgt cgaagccctg cgccacctcg
     4861 tggctggaga gcggcgtgcc gcagcgcggg cagtagggaa cgatcttgtg cccctggtac
     4921 aggagattgg cgtcccagat ctgctgcaac agccaccaga ccgattcgat gtactcgttg
     4981 tggaacgtga cgtacggatg gtccatgtcc agccagtagc cgatccgttc cgtgttccgg
     5041 cgccactccg cttcgtagcg gagcacgctc gcgcggcagc gcgcgttgaa ctcggcgatg
     5101 ccgaacgtct cgatctgttc cttcccgctg atcttgagct cgcgctccac ctcgatctcg
     5161 accggcaggc cgtgcgtgtc ccagcctccc ttgcgcggca cgtagtgccc cgtcatcgac
     5221 tggtagcggc agacgatgtc cttcagggtg cgcgagatca cgtggtggac gccgggcagc
     5281 ccgttggcgg tgggcgggcc ctcgtagaag atgaacggcg gctgcccctc gcggcgcttc
     5341 aggccgtcct cgaacgcgcg gatctccttc cacagcgcga gcaccctgcc ctcggactcg
     5401 gcggggtcga ggcgcgtgga gagcggacgg aagcgttcgg cggcggcggg catcagcgct
     5461 ccgggttctc cgcgtcggaa gcggcggtgg gcatgcgctc gagcacgcgc gtgaccaggc
     5521 gcgtgagtag cggctcggcc ttggcggcgt tggcgaggat cttcggaatg tccaccgcct
     5581 cgagcgcgtc gggcaggcag agatcggtga ccacggcgaa cgcgagcacg cgcatcccac
     5641 cgtgcaccgc cgcgatcgcc tcgggaacga gcgacatgct cacgagatcg gcgcccgccc
     5701 agcggaggaa gcggtactcg gcgcgcgtct ccagattggg gcccgccacg cccaccagga
     5761 cgcccgactg cagcgcgatc cgctcttcca gcgcgacctc gtgcgccagg gcgatcagcg
     5821 agcggtcgta cggctccgac atgtcgggaa agcgcggccc caggctgtcg tgattccttc
     5881 cgatgagcgg gttgtcgccc atgaggttga tgtggtcgac gatcgccacg atgtccgcgg
     5941 gacggtagag gggattcatg ccgccggccg cgttggtggc gatcagcgtc cgcgcgccga
     6001 gcgcgcgcgt gacgcgcacc gggaagacga cctggtccat cgtccagcct tcgtagtagt
     6061 gggcgcgccc gttcagcacg gcggtgggct tgccgcccag cgtgccgagc acgaggtcgc
     6121 ccgcgtggct ctccacggcg gtcgtgggaa agccggggat ctcctcgaag gggatgacgt
     6181 cggcgcccgg catcgcctcg gcgaagcggc cgaggccggt gcccaggatg acggcgacct
     6241 cgggccggac cgcgctccgc gaggcgacga aggcccgcgc ctcttcgatc cggcgcggca
     6301 gatcgtccgc gcctttgggc gcggcgctca cgacggtgct tccagcctgc gccatacttc
     6361 ctcctcgtcc atgccgtcga tctcgatcca cttcctgcgc gaagcttcgc ctccggcgat
     6421 ccgaacggcg ctccgcggca gccccagccg ttcgcgcaac aggtcgagca ccgcgcggtt
     6481 cgcacgcccg tcctccggcg cggccgtcac ccggatccgc accgtcttct ccgcgcgcga
     6541 ggcctggatc tcgtcggcgc ggctcttggg cgccacggtc acctgcagcc gcacggcctc
     6601 aggcatccgg cccgaaaatc gcggacccca cgcggacgat cgtcgccccc tcctcgatcg
     6661 cggcctcgaa gtcgtcggtc atgcccatcg agagccccgc gtcgggagcg agagcgccgc
     6721 gagcgaccgc ctcgtcgcgg atccgtcgca gggcgcgaaa ggacacgcgc gcgcctgcct
     6781 cgcccccctc cagggggccg atggtcatga gccccgcggc gcgcagcgag cggatcgcgg
     6841 cgacgcgctc gatcagcgcc ggcgccccct cgggcgcgac gccgtgcttg cccggatccc
     6901 cggaggtgtt gacctcgacg taggcctcca gggcgcggcc cgcctccgcg gcacgccgcc
     6961 cgatctcctc ggccagacgc tcggagtcga tcgagtggat cgcgtcgaag agaagcgccg
     7021 cccgcttcgc cttgttcgtc tggaggtgcc ccaccagatg ccagcgcggg cccgggccga
     7081 cgagcccgat cttcggctcg gcctcctgga cccggctctc cccgagctcg acggcgccga
     7141 gccggaacgc ctcccggacc cgctcggcgt cgatcccttt cgacaccgcg aggaggcgca
     7201 ccgagccggc tggccggccg gaccgttcgg cggcccgcgc gatgcgctcc cggacgtcgt
     7261 cgagccggtt cgaaagcgaa gcgagatcgc gcgttccagc acccccggaa aacgtaagcc
     7321 ggacagtata gaggtgggcg gccgagggga acaacaggaa agcccccgga cggagcccgg
     7381 gggcgcccag cgaagggcgt cagacggggg aactgtctaa cgcccctcgc ggaataccga
     7441 cgtcgtgcgg aggaccgatc cctcgcagcc ccgcttccgg caggggatgc gcgacggaat
     7501 cgcgttcgga tcggacggca ccgctccgga tcgggaaacg tgcagctgaa tcagggcctg
     7561 cacctgctgc ggggtgtaca tgtacccgca tcggttgcag cgataccagt actccgtgtc
     7621 catgggtccg ctcccgtgct cgatgggcgc aaggaagcct cgaccctgtg ggggccatcg
     7681 cggaccgttc gttccgtgct gcatgccgcc gggcgcgggg cgaacgcgcc ctgccgcgga
     7741 gaccgcggcg gcgacacgct accaccggcc cacccgaaag cgacaccaaa tttgctacga
     7801 tccgcccgtt cccgctggag atccgaaacc cgcagggggg atccggtcat gggtaagatt
     7861 ctcctccact tcgtgatcat cgtgctgacg ttcctgctcc tcgcgaacgt cgttcccggg
     7921 ttcgcggtga agagctggca ggccgccgtg gtcgcggcgc tggtcttcgg gctggtgaac
     7981 gcgatcctgg ggcccgtgct caccctggtg tcgctgccgc tgatcattct gacgctcggg
     8041 ctcttctggt tcgtcgtgaa cgcgtttctc ctgatcctgg tcggcttcat cgtgcccggt
     8101 ttcgacttca acggattcct acccgccctc atcgtctcgg tcgttctcgc cgccgtgaac
     8161 ctggtctgga aggccgcctc gcgcgaacgg catcgctcct gagcgccacc ggcccgcccg
     8221 cgacgcggca caccgtgctt cgatgacctc cgagcgggtc ccgcgcgatc tggcgccgaa
     8281 ccacctgacg cggctcctcg aagcgaagcg cgccggcgcg tc
//
LOCUS       DASSLZ010000267         9420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1096166, whole genome shotgun sequence.
ACCESSION   DASSLZ010000267 DASSLZ010000000
VERSION     DASSLZ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096166"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VFM00_10085"
     CDS             <1..1133
                     /locus_tag="VFM00_10085"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HET7498638.1"
                     /translation="HGFLPLMDLGAPSWRAQIGTAACEFERHLGHPPEGFWLPECGYA
                     PGVEEVLAEFGVRWFAVDAHGILDACPRPVPGTYAPLRTKSGVLAFGRDAQSSRQVWS
                     STEGYPGDAWYREYYRDQGFDLPIEEVREFLPSGGTRTHLGLKYHRITDRRYPHREAY
                     DPARASARVAEHAAHFVGGRLAQARAASDALGQPATLLAPYDAELFGHWWYEGPDWLD
                     QVLDGVAASGELAAADPLEASTALARVPEGRLHPSSWGAGGYAAVWLDPVNDWIYRHL
                     LRAAERMQRLAGEGDRGDALRARALRQAGRELLLAQASDWAFIMKTGTLIPYAVRRTE
                     SHLDRFEQLASALEKGPLDAAAIAALEAADPLFPELDPGLFA"
     gene            1204..2403
                     /locus_tag="VFM00_10090"
     CDS             1204..2403
                     /locus_tag="VFM00_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498639.1"
                     /translation="MLRSNLVRGGILFVSLSLSLVSCGQRTPSEPDLPTRIVAVPTTD
                     VFANSPQLESRELLIDGRATDIEWNVAGIPTVVLLKGDTGRGGDYFASVRAIWTYNPF
                     NQDSVALYMLVQWSDPAPDFLEQPLITSVDWTDDDGNSLIDCETSDPLHNPANWHQNT
                     ALHEDQVEVELYSDASGDYPADRWRWGAGTTDPVTPVNPTEIPTADSTETSGSTLHPT
                     GGWSEDFYNSGSGWVRDAGRVTYEDNFTAGSAVPIYIASKGTRDIRLNRGKPTARLIW
                     RYVATLLGACDSINPVRVDDASQREKTWNPGDYVPSYISGMPSASQADVVSRGGWERG
                     KWSLELRRLLQSRDRDGTTTRGAPHLDDIELHSGRTYGIRIRIYNASKTRSSASPILP
                     LYIKPRS"
     gene            2409..4022
                     /locus_tag="VFM00_10095"
     CDS             2409..4022
                     /locus_tag="VFM00_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498640.1"
                     /translation="MARSTPHRTLPSGRGSAVALVSILLLSLAPLMVARAAAGQVAEA
                     WVHATPIEALGIWQSARLTAMGGLTVSAEDRFSRLNAYEYGMNPAGLLGLRDTSWAEQ
                     GSEYQDFADAYYGQNHSAVIRKSGIRGAVQQQKWALGLDLIYASLDASRHDMIGTPDN
                     SRFIRDFDIPFASSYQPITGDRTIGARVVYPGAALTYARRAPVLSWITLGGRIGYREE
                     SEDRRIADPYNLENTARATEYTAGAVIRLPHVGNAVRLAGFGQYVKDQVKARSQSPLN
                     DDLFDWERPTVAYGAELLIGTGRIRGIVDGRHRSYDGEQVAQVNWAPQFFMNPFPSTT
                     DTRYVFKKRWSSFLAGYRHNEASTRWMAALPWLPAHLGAEWGYYREYEWIRPNPDVLQ
                     MALPMDVRRLGYRAAGGFALDLPDGDGQIAVEVHATRDFRNDFLGVFPDIASSERSYH
                     LGAEYRVLHWLPVRAGVALLRTDPDRRDAIAPYKGIRASVGAGTYWRALGAQIDAAYA
                     HEHFHHEPLDPSLEIGSGDQAVLTISRLF"
     gene            4030..5478
                     /locus_tag="VFM00_10100"
     CDS             4030..5478
                     /locus_tag="VFM00_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET7498641.1"
                     /translation="MDELVREALDTAVARGAGYADARLLETHREDLIVKDGRLAGLEQ
                     TESRGIGVRVLVDGAWGYAATDATSKESVQACAAHAVAIGRASARVLTNPVRLAPEPA
                     HRAVWTSPCVVDPFAVPLDRKLDLLLRIDAALRAVKGVSIAEGFLTFVRRRQRFLSSE
                     GADIDQTVTRSGGGYAATAVSGNEVQRRSYPQAEGHHQTMGWEFIESIPWVEEAPRVA
                     EEAVALLGADPCPAGEWDVVLEGSQLALQIHESVGHPSELDRVLGMEANYAGASFLTL
                     DKLRTLRFGSPKVTLVADATLPHGLATFGFDDEGVAAQRWPIVQEGLFTGYLTSRELA
                     HRVGEARSRGCVRTDGWHHIPMIRMVNLSLMPGAGSLEDLLGDTDRGIYMETNRSWSI
                     DQLRYNFQFKTEIAWEIRRGKRVRMLRNPTYQGITTQFWNSCDFVCGEEAWRPWGVVN
                     CGKGQPGQVADMTHGAAPARFRKVRVGVGYDD"
     gene            5471..6817
                     /locus_tag="VFM00_10105"
     CDS             5471..6817
                     /locus_tag="VFM00_10105"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET7498642.1"
                     /translation="MTRDEAQDVLGRALAAAKGLEMEAVLGGGRSELTRFANNEITQN
                     VSERRYVLSVRVARGRRTGRATGNDLSDDGIARLVRRADDAARLSPEIEDLLPLAEPQ
                     EYQKVDALDPATASLGPDARAAAVERAVARCRDAGLEAAGIYESGAGSIGEYGTIAPL
                     AIANTKGLFAHHDGTAARFTISAMEGAASGWASGESHASRDLDPDAIAGRAVEKAVRS
                     RKPERWEPGRYTVVLEPAAVADLLTDTAWLSFGALPVQEGRSFLSGKIGQPVLGANVT
                     IRDDPYHPLHRGIPFDAEGIPVTPVTIVDKGVANSPVYDRQTAAREGKASTGHGLPYP
                     NPFGPITGHLVLEGGSGSVDDLVARVERGLLVTRVWYTNVVDTMTATLTGMTRDGLFA
                     IEGGRIRGAVQNFRFNQSVVRMLNEVEALSAVERVGGVVCPGLLVRSFHMSSGTEF"
     gene            7152..>9420
                     /locus_tag="VFM00_10110"
     CDS             7152..>9420
                     /locus_tag="VFM00_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7498643.1"
                     /translation="MSSRRRGSARALLPLVAALLFAVAPSVLPAQSLAQTGTISGTVT
                     DGSGGPVSGVSVIILGKQMGGFTNPEGKFTIVRVPVGTYTVRANQIGFTRDEKTVQVD
                     ANRTAVVNLKIVESAIKIGVVDVKGDKKMAINKTSSSTKQVVTSEDLHALPVDSYKDA
                     IALKAGVISQGGALHFRGGREDEVLTIVNGIPSRNPMRAEGVDLGLLAVSSSEQVLGG
                     MDAQYGNALSGVIALTTREGGDKFGGEARYFTDRYGEQDKSFNDFERLSVGFGGPFVF
                     PKTNYYVSFEGTYSDTYLRNTSEHKEHRFLDFIRIGNKQSNATNFSSKLTWKVTPNQK
                     MNLEVIRNASLSSRFHNRWNRKGFVQLVADSTAPTDGTITQRYGTWAYYQVDSSFVPM
                     NTADHLPITTEDYQQVALTWRSVMNNGAIYNLRASRQEWQSKQDVLGRQLWEYQAQPN
                     AYYDPFNRLNGAYYVTNGDYPFYENRQTTTYTMNGDLSKKIGQHNFMVGGDFNYNDLQ
                     YLLTQFPNVLDANGNYGATRDQFRNFNPEGSFYAQDRWQYEGMVLNAGVRYDNFSVGS
                     QVDRNLVTHPVKTQISPRIGIAYPISDRDVMSFHYGRLFQVPDRLFIYQGKNISAEAR
                     GNPNLEPQTTIAYQLGVQHLFSKEIYGQFGVYFKDIFGLLTTVQQEIPGFSVTVPTYV
                     NGDYASSRGIEVTLIKQHSHGFSGELNYTYSNSSGTASDPNRALASSGNLRDQYKPTS
                     EQPLDWDQRHSISATL"
BASE COUNT         1570 a         3320 c         3126 g         1404 t
ORIGIN      
        1 cgcacggctt cctgccgctc atggatctgg gcgcgccctc gtggcgggcg cagatcggaa
       61 cggccgcgtg cgagttcgag cggcatctcg ggcatcctcc ggagggcttc tggcttccgg
      121 agtgcggcta cgcgccgggc gtggaggaag tgctcgcgga gttcggcgtg cgctggttcg
      181 ccgtggacgc gcacggcatc ctcgacgcct gcccgcgccc cgtcccggga acctacgcgc
      241 cgctccggac gaagtcggga gtgctcgcct tcggccgcga cgcccagtcc tcgcgccagg
      301 tctggagcag caccgagggc tatccgggcg acgcctggta tcgcgagtac taccgcgacc
      361 agggcttcga tcttccgatc gaggaagtcc gggagttcct gccatcgggc ggcacgcgca
      421 cccacctcgg gctcaagtac caccgcatca ccgaccgccg ctacccgcac cgcgaggcgt
      481 acgatcccgc gcgcgcgtcg gcgcgcgtgg ccgaacacgc ggcgcacttc gtcggcgggc
      541 ggctcgcgca ggcgcgggcg gcctcggacg cgctgggcca gccggcgacg ctgctcgcgc
      601 cttacgacgc ggagctgttc ggccactggt ggtacgaggg acccgattgg ctcgaccagg
      661 tgctcgacgg cgtggccgcg agcggggaac tggccgcggc cgacccgctc gaggcctcga
      721 cggcgctcgc acgcgtgccc gaaggaaggc tccatccctc ttcctggggc gcgggcggat
      781 acgcggcggt gtggctggac ccggtgaacg actggatcta ccggcacctg ctccgcgccg
      841 ccgagcggat gcagcgcctc gccggggaag gggatcgcgg cgacgcgctg cgcgcgcggg
      901 cgctccggca ggccgggcgc gagctgctcc tggcgcaggc gagtgattgg gccttcatca
      961 tgaagaccgg gacgctgatc ccgtacgccg tccgccgcac cgagtcgcat ctcgaccgct
     1021 tcgagcagct ggcctcggcg ctggagaagg ggccgctcga cgcggcggcg atcgcggccc
     1081 tggaagcggc cgatcccctg ttcccggaac tcgatccggg gcttttcgct tgacacatgg
     1141 cgcggcccgt tcgtaggcta cgaacgttcc ccccctgaac gtcgttgatc aatcgaggac
     1201 tacatgctcc gcagtaacct cgtacgtggt gggattcttt ttgtctcgtt gtccttgtca
     1261 ctcgtttcct gcggccagcg cacgcccagc gagccggatc ttccgacccg catcgtcgcc
     1321 gtgccgacca ccgacgtctt cgccaactcg ccccagctcg aatcgcgaga gctcctgatc
     1381 gacggccgcg ccaccgacat cgagtggaac gtggccggga tacccaccgt ggtgctcctg
     1441 aagggcgaca ccgggcgggg aggcgactac ttcgcgtcgg tgcgcgcgat ctggacctac
     1501 aatccgttca atcaggattc ggtcgcgctc tacatgctgg tccagtggtc cgatcccgcg
     1561 cccgacttcc tcgagcagcc cctgatcacg tcggtcgact ggaccgacga cgacgggaat
     1621 tcgctcatcg attgcgagac cagcgatccg ctccacaacc cggccaactg gcatcagaac
     1681 accgcccttc acgaagacca ggtcgaggtg gagctctaca gcgacgcgag cggggactac
     1741 ccggccgacc gctggcgatg gggcgcgggc acgaccgatc ccgtcacgcc cgtgaacccg
     1801 accgagattc cgacggcgga ctccaccgaa acctccggtt cgacgctcca tcccacgggt
     1861 ggatggtcgg aggacttcta caacagcggg tcggggtggg tgcgcgacgc cgggcgggtc
     1921 acctatgagg acaacttcac cgccggcagc gccgtcccga tctatatcgc gagcaagggg
     1981 acgcgcgaca tccgtctgaa ccggggcaag ccgacggccc ggctcatctg gcggtacgtc
     2041 gcgacgctgc tcggcgcctg cgacagcatc aacccggtcc gcgtcgacga cgcgtcgcag
     2101 cgggagaaga cctggaaccc gggcgattac gtgccctcgt acatcagcgg catgcccagc
     2161 gccagccagg cggacgtggt ctcgcggggc gggtgggagc gcggcaagtg gtcgctcgaa
     2221 ctgcggcgcc tgctgcagtc gcgcgaccgc gacggcacga ccacgcgcgg cgcgccccat
     2281 ctggacgaca tcgagcttca cagcggccgc acgtacggga ttcggatccg catctacaac
     2341 gcgtccaaga cgcggagcag cgcgtccccg attctccccc tctacatcaa gccgagaagc
     2401 tgagcgccat ggcccgatcg acgccacacc gaacgcttcc gagcggccgc ggctccgccg
     2461 tggccctggt ttccatcctt ctcctttcgc tcgcgccgct gatggtcgcg cgcgcggcgg
     2521 ccggccaggt ggccgaggcc tgggtgcacg cgaccccgat cgaagcgctg ggcatctggc
     2581 agtcggcgcg cctcacggcc atgggcggac tcacggtctc ggccgaggac cgcttcagcc
     2641 ggctgaacgc ctacgagtac ggcatgaacc cggccgggct cctggggctc cgcgacacct
     2701 cctgggcgga gcagggatcc gagtaccagg atttcgccga cgcctattac ggccagaacc
     2761 acagcgccgt catccggaag agcgggatcc ggggcgcggt gcagcagcag aaatgggccc
     2821 tcgggctgga cctgatctac gcgtcgctgg acgcgagccg ccacgacatg atcggaacgc
     2881 ccgacaacag ccgcttcatc cgcgacttcg acattccctt cgccagctcg taccagccga
     2941 tcaccggcga ccggacgatc ggcgctcggg tcgtctatcc cggcgcggcg ctcacctacg
     3001 cccggagagc tccggtcctc tcgtggatca cgctgggtgg acggatcgga taccgcgaag
     3061 agagcgagga ccggcgcatc gccgatccct acaatctcga gaacacggcc cgcgccacgg
     3121 agtacaccgc gggcgccgtc atccgcctcc cgcatgtcgg aaacgccgtt cgcctggcag
     3181 gcttcggcca gtacgtgaag gaccaggtga aggcccgcag ccagtcgccg ctcaacgacg
     3241 acctcttcga ctgggagcgc cccaccgttg cctatggggc cgagctcctc atcggaaccg
     3301 gccgcatccg cggcatcgtc gatggacggc atcgctccta cgacggggag caggtggccc
     3361 aggtgaactg ggcgccgcag ttcttcatga atccgttccc ctcgaccacc gacacgcggt
     3421 acgtcttcaa gaagcggtgg tcctcgttcc tcgcgggata ccgtcacaac gaggcgagca
     3481 cgcggtggat ggcggcgctc ccgtggcttc ccgcgcacct gggcgccgag tggggctact
     3541 accgcgaata cgagtggatc cggccgaacc ccgacgtgct gcagatggcg ctcccgatgg
     3601 acgtgcgccg gctgggctac cgggccgcgg gcggcttcgc gctcgacctt ccggacggcg
     3661 acggccagat cgcggtcgag gtacacgcca cgcgcgactt ccggaacgac ttcctcggcg
     3721 tgttcccgga catcgcctcg agcgagcgga gctatcacct cggcgccgag taccgcgtcc
     3781 tgcactggct gccggtccgg gccggcgtcg cgctcctgcg caccgatccc gaccgccgcg
     3841 acgccatcgc gccgtacaag ggcatccgcg cctcggtggg cgcggggacc tactggcggg
     3901 cgctgggcgc ccagatcgac gcggcctacg cccacgagca tttccatcac gagccgctgg
     3961 atccttcgct ggaaatcggc tcgggcgacc aggccgtcct gaccatctcc cgactcttct
     4021 agacgcttca tggacgagct cgtccgcgag gcgctcgaca ccgcggtcgc gcgcggcgcc
     4081 ggctatgccg acgcgcgcct gctcgagacc caccgcgaag acctgatcgt caaggacggc
     4141 cgcctggccg gcctggagca gaccgaatcc cgcggcatcg gcgtgcgcgt cctggtggat
     4201 ggagcgtggg gctacgccgc caccgacgcc accagcaagg agagcgtgca ggcgtgcgcc
     4261 gcgcacgcgg tggcgatcgg ccgggcgagc gcccgcgtgc tgacgaatcc ggtgcggctc
     4321 gcgcccgagc ccgcccatcg cgccgtctgg acctctccct gcgtcgtcga tcccttcgcc
     4381 gtgccgctcg accggaagct cgatctcctg ctccgcatcg acgcggcgct ccgcgccgtg
     4441 aaaggcgtct ccatcgccga ggggttcctc acgttcgtcc gccgccgcca gcgcttcctc
     4501 tcctccgagg gcgccgacat cgaccagacg gtcacgcgct ccggcggcgg ctacgcggcc
     4561 accgcggtct cgggcaacga agtgcagcgc cgctcctatc cccaggccga aggacaccac
     4621 cagacgatgg ggtgggagtt catcgagtcg atcccctggg tcgaagaagc gccgcgcgtg
     4681 gcggaagagg cggtcgcgct cctcggggcc gatccctgcc cggcggggga gtgggacgtc
     4741 gtgctggagg gttcccagct cgcgctgcag atccacgagt cggtcgggca tccttcggag
     4801 ctcgaccggg tgctggggat ggaggcgaac tacgcgggcg cctccttcct cacgctcgac
     4861 aagctgcgca cgctccgctt cggatcgccg aaggtgacgc tcgtcgccga cgccaccctg
     4921 ccgcacgggc tcgccacgtt cggcttcgac gacgaggggg tggcggcgca gcgatggccg
     4981 atcgtccagg aggggctctt caccgggtac ctcacctcgc gcgagctggc gcatcgggtg
     5041 ggggaggcac gcagccgcgg ctgcgtgcgc accgacggct ggcaccacat cccgatgatc
     5101 cggatggtga acctgagcct catgcccggc gcgggctcgc tcgaggatct gctcggcgac
     5161 accgaccgtg ggatctacat ggagaccaac cgcagctgga gcatcgacca gctccgctac
     5221 aacttccagt tcaagaccga gatcgcgtgg gagatccgcc gcgggaagcg cgtgcgcatg
     5281 ctgcggaatc ccacctacca ggggatcacg acgcagttct ggaattcctg cgacttcgtc
     5341 tgcggggagg aggcgtggcg cccctggggc gtcgtgaact gcgggaaggg acagccgggc
     5401 caggtggccg acatgaccca cggcgcggcc cccgcccgct tccggaaggt gcgcgtcgga
     5461 gtcggctacg atgactaggg acgaggccca ggacgtgctc ggccgggccc tcgccgccgc
     5521 gaaagggctc gagatggagg cggtgctcgg cggaggccgc tccgagctca cccggttcgc
     5581 caacaacgag atcacccaga acgtctccga gcgccgctac gtgctctccg tgcgcgtggc
     5641 gcgcggccgg cgcacgggac gcgcgaccgg aaacgacctg agcgacgacg gcatcgcgcg
     5701 gctggtgcgc cgcgcggacg acgcggcgcg cctctctccc gagatcgagg atctgctccc
     5761 cctcgcggag ccgcaagaat accagaaggt cgacgcgctc gacccggcga ccgcctccct
     5821 cggtcccgac gcgcgcgccg cggcggtgga gcgcgccgtg gcgcggtgcc gggacgcggg
     5881 gctggaagcc gccggcatct acgaaagcgg ggcgggctcg atcggggagt acggaaccat
     5941 cgcgccgctg gccatcgcga acacgaaggg cctcttcgcg caccacgacg gcaccgcggc
     6001 gcgcttcacg atctcggcga tggagggggc cgcctccggc tgggcgtccg gcgagtcgca
     6061 cgcgagccgc gacctggatc ccgacgccat cgcggggcgc gccgtcgaga aagccgtccg
     6121 ttcgcggaag ccggagcggt gggagccggg gcgctacacc gtggtgctcg agccggccgc
     6181 cgtcgcggac cttctcaccg acaccgcctg gctctccttc ggcgcgctgc cggtccagga
     6241 agggcgaagc tttctgtcgg ggaagatcgg ccagccggtg ctgggcgcga acgtcacgat
     6301 ccgcgacgac ccctaccatc cgctccaccg cggcattcct ttcgacgccg aggggattcc
     6361 ggtcaccccc gtgaccatcg tggacaaggg ggtggcgaac agcccggtct acgatcggca
     6421 gacggcggcc cgggagggga aggcgtccac ggggcacgga ctcccatatc ccaatccgtt
     6481 cggcccgatc accggccacc tggtgctgga gggaggctcg ggaagcgtgg acgatctcgt
     6541 ggcgcgggtg gaacgcggcc tcctggtcac ccgggtctgg tacacgaacg tcgtggacac
     6601 catgacggcg accctgaccg gcatgacccg cgacgggctc ttcgcgatcg agggggggag
     6661 gatccggggt gcggtgcaga actttcgctt caatcagagc gtggtccgga tgctgaacga
     6721 ggtggaggcg ctctccgcgg tggagcgggt gggcggcgtg gtctgtccgg gactcctcgt
     6781 ccggtcgttc cacatgtcca gcgggacgga attttgagct cggggccgtc cggcagcgcg
     6841 gaaatgtgaa attacccctt gcgggccgca cgaacctgaa tataatcctg gcttagagac
     6901 gagttccttc ctgccggacg tctctggaaa tccccccccg caaccatcgg cttcgttcct
     6961 tgccgcccca tgcgccggcc gtttcggctt tctgggcgga gggggccgcc ggatgggctt
     7021 cccgaagccc cgaacactcg aaatccgaag ggttttgcct tgacgagaac cgctcaggac
     7081 ggcctagaat cagccacttt gtcacgattt cgaacccgcc atggaaagga gcgcatgaag
     7141 ccatgccgag catgagttcc cgacgacgcg ggagcgcccg ggcgcttctg cccctggtcg
     7201 ccgcgttgct gttcgccgtc gcgccgtccg tccttcccgc ccaatcgctg gcgcagaccg
     7261 gaaccatcag cggaaccgtt accgatggca gcggcggtcc cgtatccggc gtctccgtca
     7321 tcatcctcgg gaagcagatg ggtggcttca cgaatcccga agggaagttc accatcgttc
     7381 gcgttcccgt gggcacgtac accgtccgcg cgaatcagat cggattcacg cgcgacgaaa
     7441 agaccgtcca ggtggacgcc aaccgcacgg cggtcgtgaa tctgaagatc gtcgagtccg
     7501 ccatcaagat cggcgtcgtc gatgtgaagg gcgacaagaa gatggcgatc aacaagacca
     7561 gctcctcgac caagcaggtc gtcacctccg aggatctgca cgccctcccc gtggacagct
     7621 acaaggacgc gatcgccctg aaggccggag tcatcagcca gggcggcgcg ctgcacttcc
     7681 gcggcggccg tgaggacgag gtgctcacca tcgtcaacgg catcccgagc cggaacccga
     7741 tgcgcgccga gggcgtggac ctcgggctcc tcgccgtctc ttccagcgag caggtgctcg
     7801 gcgggatgga cgcgcagtac gggaacgccc tctccggcgt catcgcgctc accacgcgcg
     7861 agggaggcga caagttcggc ggcgaggcgc gctacttcac cgaccgctac ggcgagcagg
     7921 acaagtcgtt caacgacttc gagcggctca gcgtgggctt cggcggaccg ttcgtcttcc
     7981 ccaagaccaa ctactacgtc tccttcgagg gcacctacag cgacacgtat ctccggaata
     8041 cttcggagca caaggagcac cgcttcctcg acttcatccg gatcggcaac aagcagtcca
     8101 acgcgaccaa cttctcgagc aagctgacct ggaaggtgac cccgaaccag aagatgaatc
     8161 tcgaggtgat ccgcaacgcc tcgctcagca gccgcttcca caatcggtgg aatcggaagg
     8221 gattcgtgca gctggtcgcg gacagcacgg cgccgacgga tggaacgatc acgcagcggt
     8281 acggcacgtg ggcgtactac caggtcgact cctcgttcgt gccgatgaac accgcggacc
     8341 acctgccgat cacgaccgag gactatcagc aggtcgcgct gacctggcgc agcgtcatga
     8401 acaacggcgc gatctacaac ctgcgggcct cgcggcagga atggcagtcg aagcaggacg
     8461 tgctggggcg gcagctctgg gagtaccagg cgcagcccaa cgcctactac gacccgttca
     8521 accgactgaa cggcgcctac tacgtcacga acggcgacta ccccttctac gagaaccgcc
     8581 agaccacgac ctacaccatg aacggcgacc tctcgaagaa gatcggccag cacaacttca
     8641 tggtcggcgg cgatttcaac tacaacgacc tgcagtatct gctgacgcag ttcccgaacg
     8701 tgctcgacgc caacggcaat tacggcgcga cgcgcgacca gttccggaac ttcaaccccg
     8761 agggctcctt ctacgcccag gatcgctggc agtacgaggg gatggtcctg aacgcgggag
     8821 tgcgctacga caacttctcg gtggggagcc aggtcgatcg caatctcgtg acccacccgg
     8881 tcaagaccca gatcagcccg cgcatcggaa tcgcctatcc gatctccgac cgcgacgtga
     8941 tgagcttcca ctacggccgc ctcttccagg tgcccgaccg gctcttcatc tatcagggca
     9001 agaacatctc ggccgaggcg cgcgggaatc cgaatctcga gccgcagacc accatcgcct
     9061 accagctggg cgtgcagcac ctcttcagca aggagatcta cgggcagttc ggcgtctatt
     9121 tcaaagacat cttcggcctc ctgaccacgg tgcagcagga gattcccggc ttctcggtca
     9181 cggtgcccac ctacgtgaac ggcgactacg cctcctcgcg cggcatcgag gtgaccctga
     9241 tcaagcagca cagccacggc ttctcgggcg agctcaacta cacctactcg aactcgtcgg
     9301 gcacggcgtc ggaccccaac cgggcgctcg cgtcgtccgg aaacctccgc gaccagtaca
     9361 agccgacctc cgagcagccg ctcgactggg accagcgcca cagcatctcg gccacgctcc
//
LOCUS       DASSLZ010000268         6940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_479466, whole genome shotgun sequence.
ACCESSION   DASSLZ010000268 DASSLZ010000000
VERSION     DASSLZ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479466"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..866)
                     /locus_tag="VFM00_10115"
     CDS             complement(114..866)
                     /locus_tag="VFM00_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7498644.1"
                     /translation="MRPLRTRHPSRVSLLFVVLLVSLAAGPFAPAPAKASFLTRDVKS
                     LTSRRTADILAGAAVLGAASLLVENPDAEEHALGRGAVDAPADVGNIYGSGLVLAGVT
                     LGLTGTGLVEKDPDWVRAGSEMARSLAYAGIAVTALKVAVRRTRPNGGAYSFPSGHTA
                     AAFAVAPVLARRFGWLAALPAYALATSTALGRMEDRKHYLSDVVVGAGIGTAIGLAVA
                     SKRPPSAAEPESVKPSLGLGIAPTGLSLVAKF"
     gene            989..1951
                     /locus_tag="VFM00_10120"
     CDS             989..1951
                     /locus_tag="VFM00_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498645.1"
                     /translation="MSDPVSSPSYRPIGEEPSGASALLRHVLPLLLVAVAAVGFLAPQ
                     VLRRLLPGVEPFAARTAAIVGLFLLVAITGIRDGRRRLAARRSAFASFAASVGGRVTM
                     RPLVAGAPAWEAAEHVEYDAQGRPAMLSALRGKEASFAFRLAADTALRRDFQFQVLPG
                     GRAMRFVLSKSFLLPVLSVAVKTSGSARVSVRRAAAGGASGSLTDPAQLIERMRYLAD
                     DAVTTGDREFDRAYLVKASDRDAGRAVASDPGFRAAIAALAGRASTFQAGLESESAEG
                     PARIVIAATADASPELFLAMDGALRATAVALARAGIVDGARGVA"
     gene            1979..3088
                     /locus_tag="VFM00_10125"
     CDS             1979..3088
                     /locus_tag="VFM00_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498646.1"
                     /translation="MTSDSAAPRAIRRLPLVVRDAEAGRNAERFFVAAIATLLGLRAL
                     LAATGYPQIGGRHLHIAHMLWGGLVLTAALLLVLVSIGRRTRPAAAFLGGVGFGLFID
                     ELGKFLTRDSDYFYRPAISIIYFVFLTLFLGMRALLARRQLTEDECLANAITLIKDMA
                     LEDLDPDEKAQAATLLGRCDPQDARVIAVRGFLDRLNTVPAPPPGLLERARHRLQPRV
                     ERILTQPRFPRALAGILFALAVSGLAWAFSTARGLPMTGRPAAQTHLDIGFASWGEIA
                     SNGIACVLMMAGIVRLFGNRNAAYRLFRRSVRVTVFVTQFFVFYHAQMAGVVWLGLNV
                     VALGVLDYLIAREEKAPAVLAARRQAQASTATDPA"
     gene            complement(3058..3198)
                     /locus_tag="VFM00_10130"
     CDS             complement(3058..3198)
                     /locus_tag="VFM00_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498647.1"
                     /translation="MAVFPCVPARSAFLLVAILSLAAVPLAFLLRGHKSGDQAGSVAV
                     EA"
     gene            3221..3589
                     /locus_tag="VFM00_10135"
     CDS             3221..3589
                     /locus_tag="VFM00_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET7498648.1"
                     /translation="MEAGVRVLARVEGLTPGKHGFHIHEKGDCTAPDASSAGGHFNPN
                     NGPHGAPTADRSARHAGDLGNLEAGPDGKARYDRVDTVLDFAQLAGLSVLVHAGEDDL
                     TSQPAGNSGARVACGVFRMK"
     gene            complement(3628..4317)
                     /locus_tag="VFM00_10140"
     CDS             complement(3628..4317)
                     /locus_tag="VFM00_10140"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HET7498649.1"
                     /translation="MHPKLIRFAAAILFGVAALAPSARAADPVENDEAERLDRARVAF
                     QEIRDMKDENIPRPLMERARCVAVFPGMVKGALGWGARVGKGVMSCRDASGHWGPPIF
                     LTVKGGSFGFQIGVEKADVVLFFMTDKSARSLMESKFTLGGKAGLAAGPVGRTAEAST
                     DLKLDAEIYSYARSKGLFAGISLEGARVAPDEKATRRFYGPSADSRKILREHAAPANP
                     PAARAFVEALP"
     gene            4432..5127
                     /locus_tag="VFM00_10145"
     CDS             4432..5127
                     /locus_tag="VFM00_10145"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7498650.1"
                     /translation="MTDPQATRLYLARHGATPLTAEDRFSGDEGVELSDEGRRQASLL
                     GERLRGEGIAAVYASPLSRAMDTARLACAACGEAGLRPLARDGLREIRHGHWEGKSRT
                     EVEARFGSEYAAWEADPFTFAPEGGESGVQVLARALPVIREIVTRHEGARVLVVSHKA
                     TIRLVLSSLLGIDARGYRDRLDQAPAALNVLDFKGPVRARLMLFNDTSHYASRPRVPE
                     RNLSKWWDRERAE"
     gene            complement(5129..5503)
                     /locus_tag="VFM00_10150"
     CDS             complement(5129..5503)
                     /locus_tag="VFM00_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HET7498651.1"
                     /translation="MKSPALFGVLGAVFGFLGVAAGAFGAHALRDTLPPDRLQVFETG
                     ARYQLIHALALIAVALALDRAASGALTAAGWLLVLGQVIFAGSLYALALSGVRLWGAV
                     TPLGGLCYLVGWILLAIAFAKR"
     gene            complement(5500..6423)
                     /locus_tag="VFM00_10155"
     CDS             complement(5500..6423)
                     /locus_tag="VFM00_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7498652.1"
                     /translation="MTRSAPLPDIDSVWDFDHPDASEAAFAALLPLARASGDPDYLAQ
                     LLTQVARAEGLQMKFDHASKTLDEAEAMITAETPAARVRLLLERGRVLNSSKRGEESK
                     PFFLEAWDLARETGAGGLAVDAAHMLGIVERGDASVAWNEKAIAYAEASNDPKARRWL
                     GSLYNNLGWTWHDAGDAERALGLFQRALAAREAEGKPGLIRVGRWCVARALRSLGRLD
                     EALAIQRRLLAENEAHAPDGYVHEEIGEILLARGDESGAGPHFAAAWKVLSEDPWLRR
                     DEADRLERMWRLGGLPGATSGAADPPSSGAR"
     gene            complement(6420..>6940)
                     /locus_tag="VFM00_10160"
     CDS             complement(6420..>6940)
                     /locus_tag="VFM00_10160"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HET7498653.1"
                     /translation="GVERGPEDQGPGTFASSAEAMRGVVERLERLDPERARYIARFAY
                     VLGRVANADLEVTEMETRAMERLVQSLGGLEEDEAVLVVEIAKVQHRLFGATDSYSIT
                     RDFAKTATAEQKREVLECLFAVSAADENVSGVEENEIRRIVKELDLAHEDFIAARASY
                     REHVARLREETP"
BASE COUNT          969 a         2492 c         2477 g         1002 t
ORIGIN      
        1 ggagcgtctg ggccatccgg aatcggaagc gcacgatccc gctccggacc tggcgcggtt
       61 cgactcgatc gcggagtggg tggcggaggt cacggagcgc taccgggagt aactcagaac
      121 ttcgcgacga gggaaaggcc ggtgggagcg atgccgagcc cgagcgacgg cttgacgctc
      181 tcgggctccg cggccgaagg cgggcgcttc gaggccacgg ccagcccgat cgccgtgccg
      241 atgcccgcgc ccacgaccac gtccgacagg tagtgcttcc gatcttccat ccgtccgagc
      301 gccgtgctcg tggcgagcgc gtaggcgggc agggccgcca gccacccgaa ccgccgcgcc
      361 agcacgggcg ccaccgcgaa ggcggccgcc gtgtggccgg aagggaagga gtaggcgccg
      421 ccgttgggac gcgtccggcg cacggcgacc ttgagcgcgg tcaccgcgat cccggcatag
      481 gcgagcgagc gcgccatctc cgacccggcg cggacccagt cggggtcctt ctccaccagt
      541 cccgttcccg tgagcccgag ggtgacgccg gcgagcacga ggccgctccc gtagatgttg
      601 ccgacatcgg cgggcgcgtc cacggcgccc cggccgaggg catgctcctc ggcgtcggga
      661 ttctcgacga ggagcgaggc ggcccccagc accgcggcgc ccgccaggat gtcggcggtc
      721 cggcggctgg tcaggctctt cacgtcccgc gtcaggaatg aggccttcgc cggagcgggg
      781 gcgaagggac ccgccgccag ggaaacgaga agaacgacaa agaggaggga gactcgggac
      841 gggtgcctcg tgcggagggg gcgcatgaag tgccctgtcc taccccgtgg agggagcgcc
      901 ggttccggtc cagaagcgcc gcgcggatgc cgattcctgt cgtcgaaggc cgcgggctcc
      961 ctccgcgcgc cgccggaggg aggaatcgat gagcgatccc gtatcgagcc cgtcctatcg
     1021 cccgatcggc gaggagccga gcggcgcctc ggcactgctg cgccacgtcc tgcccctcct
     1081 gctcgtggcc gtcgccgccg tcggcttcct cgcgccgcag gtcctgcggc gccttctgcc
     1141 cggagtggag ccgttcgcgg cgcgcacggc cgcgatcgtc gggctcttcc tcctcgtcgc
     1201 gatcaccggc attcgcgacg gccgccggcg gctcgcggcg cgccgctccg cgttcgcctc
     1261 cttcgccgcg agcgtcgggg ggcgcgtcac gatgcgcccg cttgtcgccg gcgccccggc
     1321 atgggaagcc gccgagcacg tggagtacga cgcccagggt cgcccggcca tgctcagcgc
     1381 cctccgcggc aaggaagcct cgttcgcgtt ccgcctcgcc gccgacacag cactccgccg
     1441 cgatttccag tttcaagtcc tgccgggcgg gagagcgatg cgcttcgtgc tctccaagtc
     1501 gttcctcctt ccggtgcttt cggtggcggt gaagacttcg ggaagcgccc gcgtgagcgt
     1561 acggcgcgcg gcggccgggg gagcttccgg atccctgacg gatccggcgc agctcatcga
     1621 gcgcatgcgc tacctggcgg acgatgccgt cacgaccgga gacagggagt tcgaccgcgc
     1681 gtatctcgtc aaggcgagcg accgggacgc gggacgcgcg gtcgcgtccg atcccgggtt
     1741 ccgcgccgcg atcgcggcgc tcgcgggccg ggcgtccacc ttccaggccg ggcttgagtc
     1801 ggagtcggcg gaagggccgg cccggatcgt gatcgccgcg acggccgacg cctcgccgga
     1861 gctcttcctc gccatggacg gagcgctgcg cgcgacggcc gtcgcgctcg cgcgggccgg
     1921 gatcgtcgat ggcgcgcgcg gcgtggccta gcggggactc ctccgctacc atccccccat
     1981 gacttcggac agcgccgcgc cgcgcgcgat ccgccgcctt ccgctcgtcg tccgggacgc
     2041 cgaagcggga cggaacgcgg agcggttctt cgtcgcggcc atcgcgaccc tcctcggatt
     2101 gcgggcgctg ctcgccgcca ccggctaccc gcagatcggc gggcgccacc tccacatcgc
     2161 gcacatgctg tggggcggcc tggtgctcac cgccgcactc ctcctcgttc tggtctcgat
     2221 cggacggcgc acgcgtccgg ccgccgcctt cctgggcggc gtgggattcg ggctcttcat
     2281 cgacgagctg gggaagttcc tgacgcgcga cagtgactat ttctatcgcc ctgccatatc
     2341 aatcatttac ttcgtatttc tcacgttatt tctagggatg cgcgcgctgc tggcgcggcg
     2401 ccagctgacc gaggacgaat gtctggccaa cgccatcacg ctgatcaagg acatggcgct
     2461 ggaagatctc gatcccgacg agaaggcgca ggccgcgacg ctgctcggcc ggtgtgatcc
     2521 ccaagacgcg cgcgtgatcg ccgtccgtgg cttcctcgac cggctgaaca cggttcccgc
     2581 cccgccgccc ggcctgctcg agcgggcgcg gcatcggctc cagcctcggg tggagcggat
     2641 tctcacgcag ccgcgctttc cccgcgccct cgcggggatc ctgttcgcgc tcgcggtctc
     2701 gggattggcg tgggctttct ccaccgcgcg cgggctgccc atgaccggcc ggcccgcggc
     2761 gcagacccat ctcgacatcg ggttcgcgag ctggggggag atcgcctcga acgggatcgc
     2821 gtgcgtgctg atgatggcgg ggatcgtgcg gctcttcgga aaccggaacg cggcgtaccg
     2881 gctctttcga cgctcggtcc gcgtgaccgt gttcgtgacg cagttcttcg tcttctacca
     2941 cgcgcagatg gcgggcgtgg tgtggctggg gctcaacgtc gtcgcgctcg gtgtgctgga
     3001 ctacctgatc gcgcgcgagg agaaggcgcc cgccgtcctc gcggcgcggc ggcaggctca
     3061 ggcttcgacg gcgaccgacc cggcctgatc gccgctcttg tgcccccgca ggaggaacgc
     3121 gagcggcacg gcggcaaggg agagaatcgc gacgagcagg aacgccgatc tcgcgggaac
     3181 gcaggggaac accgccaagg gtgaagtccg attctacaag gtggaggcag gcgttcgggt
     3241 cctcgcccgc gtggagggac tgacgcccgg caagcacggc ttccacatcc acgagaaagg
     3301 ggactgcacc gcgcccgacg cgtcgtcggc gggggggcac ttcaacccca acaacgggcc
     3361 gcatggcgca cccaccgccg accgatcagc gcgtcacgcc ggcgaccttg gcaacctcga
     3421 ggccgggccc gacggcaagg cgcgctacga ccgcgtcgac accgttctcg atttcgctca
     3481 actcgccggc ctctccgtgc tggttcacgc cggtgaggac gacctcacgt cccagcccgc
     3541 tggcaattcg ggcgcgcgcg tcgcgtgcgg tgtgttccgg atgaagtgat cgcgccgtgg
     3601 tcgggcgatc gctccgggga cgaagcgcta cggaagcgcc tccacgaacg cacgggccgc
     3661 gggcggattc gcgggcgccg cgtgctcccg gaggatcttc ctcgagtccg ccgaggggcc
     3721 gtagaaccgg cgcgtggcct tctcgtcggg ggcgacccgc gccccttcca gcgagatccc
     3781 cgcgaagagc cctttcgatc gcgcgtagga atagatctcg gcgtccagct tgagatcggt
     3841 ggaggcctcg gcggtccggc ccaccggccc cgccgccagc cccgccttgc cgccgagcgt
     3901 gaacttgctc tccatcaagg agcgggcgct cttgtcggtc atgaagaaga gcacgacgtc
     3961 ggccttctcc accccgatct ggaacccgaa gctgcccccc ttcaccgtga ggaagatcgg
     4021 gggaccccag tgtcccgagg cgtcccggca gctcatcacc cccttgccga cgcgcgcgcc
     4081 ccagcccagc gctcccttca ccatgccggg gaagacggcg acgcagcgtg cgcgctccat
     4141 cagcgggcgg gggatgttct cgtccttcat gtcgcggatc tcctggaagg cgacgcgggc
     4201 cctgtcgagg cgctcggctt cgtcgttctc gacgggatcg gcggcgcgcg cggagggcgc
     4261 aagagcggcg acgccgaaca ggattgcggc ggcgaatcgg atcagcttgg ggtgcatcgt
     4321 ggcctccatg gctcggggta tcggtcgctt catgctgccc attaactgta gtccacgccg
     4381 gtcccgatcg cgctacccta cgtgcgtgcg acgagcccga gaacagcgac cgtgaccgac
     4441 cctcaggcga cgcgcctcta tctcgcccgg cacggcgcca cgccgctgac ggccgaggac
     4501 cgcttctccg gagacgaggg cgtcgagctc tcggacgagg gccgccggca ggcgtcgctc
     4561 ctgggcgagc gccttcgcgg cgaggggatc gcggccgtct acgcgagtcc gctgtcgcgc
     4621 gccatggaca cggcgcgcct cgcctgcgcc gcgtgcggcg aagcgggcct ccggccgctg
     4681 gcgcgcgacg gcctgcggga gatccggcac ggacactggg aagggaaaag ccgcaccgag
     4741 gtggaggcgc gcttcgggag cgagtatgcg gcctgggagg ccgatccctt caccttcgcg
     4801 ccggagggag gggagtcggg cgtccaggtg ctcgcgcggg cgctgccggt gatccgggag
     4861 atcgtcacgc gccacgaagg agcgcgcgtc ctcgtggtgt cgcacaaggc gaccatccgc
     4921 ctcgtgctct ccagcctgct cgggatcgac gcgcgcggat accgcgaccg cctggatcag
     4981 gcgccggccg cgctcaacgt gctcgacttc aagggccccg tccgcgcccg cctcatgctc
     5041 ttcaacgaca cgtcccacta cgccagccgg ccgcgcgtgc cggagcggaa cctctcgaag
     5101 tggtgggacc gggagcgggc ggagtaggtc agcgcttcgc gaaggcgatc gcgagcagga
     5161 tccatcccac gagatagcag aggccgccca gcggcgtgac ggcgccccag aggcggacgc
     5221 cgctcagcgc gagcgcatag agacttcccg cgaagatcac ctggcccagc accaagagcc
     5281 agccggccgc ggtcagagcg ccggacgcgg cgcggtccag cgcgagcgcg accgcgatca
     5341 gggccagcgc gtggatcagc tggtagcgcg cgcccgtctc gaagacctgg agccgatccg
     5401 gcggaagcgt gtcgcggagg gcgtgcgcgc cgaaggcgcc ggccgccacg cccaggaagc
     5461 cgaagacggc gcccaggacg ccgaagagcg cgggagactt catcgcgcgc cggaagaagg
     5521 cggatcggcc gcgccggagg tcgctcctgg gaggcctccc agccgccaca tccgctccag
     5581 ccggtccgcc tcgtcgcggc ggagccaggg atcctcggag aggaccttcc aggccgcggc
     5641 gaagtgcgga cccgcccccg attcgtcgcc gcgcgcgagg aggatttcgc cgatctcctc
     5701 gtggacgtag ccgtcgggcg cgtgggcttc gttctcggcg agcagccgcc gctggatcgc
     5761 gagcgcctcg tccagccttc ctagcgatcg gagcgcccgc gccacgcacc accgcccgac
     5821 gcggatgagt cccggcttcc cctcggcctc gcgcgcggcg agagccctct ggaagagccc
     5881 gagcgcgcgc tccgcgtcgc ccgcgtcgtg ccaggtccag ccgaggttgt tgtagaggga
     5941 gccgagccag cggcgcgcct tggggtcgtt cgacgcctcg gcgtaggcga tcgctttctc
     6001 gttccacgcg accgaggcgt cgccgcgttc gacgatgccg agcatgtggg cggcgtccac
     6061 cgccagcccg ccggctcccg tttcacgcgc cagatcccag gcctcgagga agaagggctt
     6121 cgattcctcg ccccgcttgg acgagttgag gacgcgtccg cgttcgagga gcaggcgcac
     6181 gcgcgccgcc ggggtctcgg cggtgatcat cgcctcggcc tcgtccaggg tcttcgaggc
     6241 gtgatcgaac ttcatctgca gtccttcggc ccgcgccacc tgggtgagca gctgagccag
     6301 gtagtcggga tcgcccgagg cgcgcgccag gggcaggagc gccgcgaacg cggcttcgga
     6361 cgcgtcggga tggtcgaagt cccagaccga gtcgatatcg ggcaacggcg cggaacgggt
     6421 catggcgtct cctcgcgcag ccgcgccacg tgctcgcggt agctcgcgcg cgcggcgatg
     6481 aagtcctcgt gggccaggtc cagctccttc acgatgcggc ggatctcgtt ctcctcgacg
     6541 cccgagacgt tctcgtccgc cgccgagacc gcgaaaaggc actcgagcac ttcgcgcttc
     6601 tgctcggcgg tggcggtctt cgcgaaatcg cgggtgatcg agtagctgtc ggtggcgccg
     6661 aacaggcggt gctgcacctt ggcgatctcg acgacgagca ccgcctcgtc ctcctccagt
     6721 ccgcccagcg actggaccag gcgctccatc gcgcgcgtct ccatctcggt cacttcgaga
     6781 tcggcgttcg cgacgcggcc cagcacgtac gcgaaccggg cgatgtagcg cgcccgctcg
     6841 gggtcgaggc gctcgagccg ctcgacgacg ccgcgcatcg cctcggccga ggaggcgaac
     6901 gtgccggggc cttgatcctc cggcccgcgc tccactccga
//
LOCUS       DASSLZ010000269         4101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1355822, whole genome shotgun sequence.
ACCESSION   DASSLZ010000269 DASSLZ010000000
VERSION     DASSLZ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355822"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..1804)
                     /locus_tag="VFM00_10165"
     CDS             complement(365..1804)
                     /locus_tag="VFM00_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7498654.1"
                     /translation="MTTTAHELRIHPFLAAGELRSGLNTVEIRNPFSGAPIARAALAG
                     RGDVMAALDAAVAAAPAAATLPSHARAAALERIRAGLLEQRDDLAALLAAEAGKPVAL
                     ARAELDRAAFVFQQGAEEAKRMGGEVIPMDLQPHGEHRWGLTRRFPLAPITAIIPFNF
                     PVLLAAHKLAPAIACGATMVLKVPPQDPLAALRLGEIVRDCGYPAGALAVLLCTNDDA
                     APLIDDPRVRMISFTGSARAGWAIRARAPTRRVVLELGGNAAVIVEPDADLAHAVARC
                     VAGGYTYAGQSCISTQRILVHASIYAQFVERFVTAVGALRTGDPLSPTTDVGPMIDAA
                     SAERAAGWVAQAVAAGARVAVGGQREGTMFSPAVLLDTTDAMLVNREEIFAPVTTVRP
                     YTDLDAAIARVNDSAYGIQAGLFTHDMRSILRAYERVEVGGLVVNDVPGFRMDHAPYG
                     GVKASGQGREGVRWAIEEMTELRLLVLGG"
     gene            complement(1809..2270)
                     /locus_tag="VFM00_10170"
     CDS             complement(1809..2270)
                     /locus_tag="VFM00_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HET7498655.1"
                     /translation="MVIWIDADAAPRDVKELVFRASKRLAIDTVLVANQRLQTPPGNP
                     LVRAVWVDGGADVADQYIATHARPGDLVVTQDVPLAAELVPRGIAVLDPRGEEHTAST
                     VGERLSVRDFMEQARLAGMVTGGPPPFDARAKQAFAAALDRALTRLRRLPG"
     gene            complement(2292..2624)
                     /locus_tag="VFM00_10175"
     CDS             complement(2292..2624)
                     /locus_tag="VFM00_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498656.1"
                     /translation="MTFKPAIWRPIAIVLSGLNLVGVGLAAGAAEPWHAGIHAALGLA
                     FGLWAQRLHSGTDASTGALEGDARLSALEADMGDVRRELSETQERVDFAERLLAQAQE
                     ARRVDPPR"
     gene            complement(2732..>4101)
                     /locus_tag="VFM00_10180"
     CDS             complement(2732..>4101)
                     /locus_tag="VFM00_10180"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET7498657.1"
                     /translation="RNKDRLKQWRYSRGVMLPHLGQAREAPQAGELFVQRDLAATLRK
                     LVAAERAALRAGKSRREAIYAAYDRFYRGDIARELVRGVKEEGGILALEDLARWKVRI
                     ETPLSTTYKGITVYKLPIWQQGPAMLQALNILENADLKAMGYNSPRYIHTLYQTMSLA
                     FADRDFYYGDPTFPPEEPVQGLLSKEYARARFGTIDPARNDPAIKPGDPYPYQGGSNP
                     FADLLARWSVVPAPDTMKRSGQQDRPATPSSSLLSDSAFSAQFHAGTTSIEAADEAGW
                     VVSITPSGGWVPAVIAGRTGVGLSQRAQSFVTREEDGPYNVIAPGKQPRVTLTPTLAL
                     KDGAPYLAFAVQGGDTQDQNLLQFFLNIVEFGMTPQQAAEAPNINSEQMRSSFGEHEA
                     RPGRLLVATSTPDRVRTALQGMGYTLDFEERTSGPINGILFDRLHQTMWGASSNHGDD
                     YGIAW"
BASE COUNT          605 a         1404 c         1459 g          633 t
ORIGIN      
        1 tcctgcgtca gcagcgaccg gaagtggtcc caatagtcgg gccccgtcag tgtcttgtag
       61 cgctcgccct cgttgccggc gatgacctcg tggatgtcga ccacgctccc catcccctcc
      121 atgtccggga cgaggaacac ccggaatccc ggctcctgcg ggatggtctt gtcgcgaaag
      181 ggggcggacg acggtcgtgt ctgggcgtga agggcggtcg cgagaccgag gagagcacac
      241 gcggcggcaa gtcgcatcgt ggaccttggg cagtgggcgt cgatggggag gtcggtacat
      301 cctagagtaa cgaagtgtcg gcacagactc taggcgcacg tcagggggcc gacggcgtgc
      361 ccggtcagcc ccccaggacc agcaagcgga gctcggtcat ctcttcgatc gcccagcgca
      421 ccccttcccg tccctggcca gaagctttga cgccgccata aggtgcgtgg tccatccgga
      481 agcccggcac gtcgttcacc accaggcccc cgacctcgac gcgctcgtat gcccggagga
      541 tcgatcgcat gtcgtgggtg aacaggccgg cctggattcc gtacgcggag tcgttcacgc
      601 gggcgatggc ggcgtcgaga tcggtgtagg gccgcaccgt ggtaaccggg gcgaagatct
      661 cctcgcggtt gaccagcatg gcgtccgtcg tatccaggag cacggcgggc gagaacatgg
      721 tgccttcgcg ttggccgccg accgccaccc gcgccccggc cgcaaccgcc tgggcgaccc
      781 accccgccgc ccgctcggcg ctcgcggcat cgatcatcgg ccccacgtcc gtggtcggcg
      841 agagcggatc gccggtgcgg agcgcgccga ccgcggtgac aaagcgctcg acgaattgcg
      901 catagatcga tgcgtgcacc agaatccgct gagtggagat gcacgactgc ccggcgtagg
      961 tgtatccgcc ggccacgcaa cgcgccacgg cgtgcgccag gtcggcgtcg ggttcgacga
     1021 tcaccgcggc gttgccgccg agctccagca ccacgcgccg ggtgggcgcg cgcgcccgga
     1081 tggcccaccc cgctcgagcc gagccggtga agctgatcat ccgcacgcgc gggtcgtcga
     1141 tcagcggtgc ggcatcgtcg ttggtgcaga gcaagaccgc gagcgcgcct gcgggatagc
     1201 cgcagtcccg aacgatctcg ccgagccgga gcgccgcgag tggatcctgc ggcggcacct
     1261 tgagcaccat cgtcgccccg caggcgatcg ctggcgcgag tttatgggcc gcgagcagca
     1321 cggggaagtt gaacgggatg atggcggtga tcggggcgag cgggaaccgg cgggtcaggc
     1381 cccagcgatg ctcaccgtgc ggctggaggt ccatcgggat gacctcgccg cccatccgtt
     1441 tcgcctcctc ggcgccctgt tgaaacacaa acgcggctcg gtccagctcg gcgcgcgcga
     1501 gcgccaccgg cttgccggcc tcggcggcga ggagggctgc cagatcgtcg cgctgctcga
     1561 gcaggcccgc tcgtatgcgc tcgagcgcgg cggcgcgggc gtgtgagggg agcgtcgccg
     1621 cggccggggc cgcggcgacg gcggcatcca gggcggccat gacgtcgcca cggcccgcga
     1681 gtgcggcccg ggcgatcggg gcgccggaaa agggatttcg gatctcgacg gtgttcaggc
     1741 cggagcggag ctcgccggcc gcgaggaagg gatggatccg gagctcgtga gccgtggtcg
     1801 tcatggcctc agccgggaag gcgccggaga cgcgtgagcg cccggtcgag cgcggccgca
     1861 aacgcctgtt tcgcccgggc gtcgaacggg ggcggtccgc cggtgaccat cccggccagg
     1921 cgggcctgct ccatgaaatc gcgcaccgac agccgttcgc cgaccgtcga cgccgtgtgc
     1981 tcctcacccc gagggtcgag caccgcgatg cccctgggca cgagctcggc tgcgagcggg
     2041 acgtcctggg tcacgacgag atcacccggg cgcgcatgag ttgcgatgta ctgatcggcc
     2101 acgtctgcgc ctccgtccac ccacacggcg cggacgagcg ggttgccggg cggcgtctgg
     2161 agccgctggt tggcgacgag cacggtgtcg atcgcgaggc gcttggaggc ccggaacacg
     2221 agctccttca cgtctcgcgg cgccgcatcg gcgtcgatcc agatgaccac cgcgcctcct
     2281 ccgaagaact cctagcgcgg cggatcgacc cggcgcgcct cctgcgcctg cgccagcagg
     2341 cgctcggcga aatcgacgcg ctcctgcgtc tcgctcagct cccgccgcac gtcgcccatg
     2401 tccgcttcga gcgccgacaa ccgggcgtct ccctccagcg ctccggtgct ggcgtccgtc
     2461 cccgaatgca ggcgctgggc ccagaggccg aaggcaaggc caagcgcggc gtgaatgccg
     2521 gcgtgccacg gctcggcggc tccggcggcc aggccgacgc ccaccaggtt gaggccgctc
     2581 aggacgatgg caatcgggcg ccagatcgcg ggtttgaacg tcatcgcgag ctccggtcga
     2641 gggaaacgta gtgcttgaag agagccacgc cagcgtgtgt gtcgtacgtc ggatcggccg
     2701 caccggtgtc aggccgagct ggaaggggcc ttcaccaggc gatgccgtag tcgtctccgt
     2761 ggttgctcga cgcgccccac atggtctggt gcaggcgatc gaacaggatg ccgtttatcg
     2821 gtccggacgt gcgctcctca aagtccaagg tatagcccat gccctggagc gcggtccgca
     2881 cgcggtcggg cgtcgaggtc gcgaccaaca gtcgcccggg ccgggcttcg tgctcgccga
     2941 aggacgatcg catctgctcg ctgttgatgt tcggcgcctc cgcggcctgc tgtggcgtca
     3001 tgccgaactc gacgatgttc aggaagaact gaagcaggtt ctggtcctgg gtgtcgccgc
     3061 cctgcaccgc gaacgccagg tagggagcgc cgtccttgag tgccagggtc ggcgtgagcg
     3121 tcacccgtgg ctgcttgccc ggcgcgatga cattgtacgg gccatcctcc tcacgcgtga
     3181 cgaagctctg cgcccgctgg ctcagcccca cgccggtgcg gcccgcgatc acggccggca
     3241 cccagccgcc gctcggcgtg atcgacacga cccacccggc ctcgtcggcg gcctcgatgg
     3301 acgtggtgcc ggcgtggaac tgcgcggaaa acgccgagtc ggacagtagc gatgagctcg
     3361 gcgtggccgg ccggtcctgc tggccgcttc gcttcatcgt gtcgggcgcg ggcacgacgc
     3421 tccagcgggc gagcagatcg gcgaaaggat tgctgccgcc ctggtacgga taggggtccc
     3481 cgggcttgat ggccgggtcg ttccgggccg gatcgatcgt gccgaaccgc gcgcgggcgt
     3541 attccttgga cagcagtccc tgaaccggtt cctccggcgg gaacgtcgga tcgccgtagt
     3601 agaaatcgcg gtcggcgaag gcgaggctca tcgtctgata cagggtgtga atgtatcggg
     3661 gcgagttgta gcccattgcc ttgaggtcgg cgttctcgag aatgttgagc gcctgcagca
     3721 tcgccggccc ctgctgccag atggggagct tgtagaccgt gatccccttg taggtcgtgc
     3781 tgaggggggt ctcgatgcga accttccagc gcgccagatc ctcgagcgcc aggattcccc
     3841 cctcttcctt cacgcctcgc acgagctccc gcgcgatgtc gccgcgatag aaccggtcgt
     3901 acgcggcgta gatcgcctcc cgcctgctct tccccgccct gagcgccgcg cgctcggctg
     3961 ccacgagctt ccgcagcgtc gccgccaggt cgcgctggac gaacagctct ccggcctgag
     4021 gcgcctcacg cgcctgcccg aggtgcggga gcatcactcc gcgggagtag cgccactgtt
     4081 tgagacgatc cttgttgcgc t
//
LOCUS       DASSLZ010000270         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1240580, whole genome shotgun sequence.
ACCESSION   DASSLZ010000270 DASSLZ010000000
VERSION     DASSLZ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240580"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1044
                     /locus_tag="VFM00_10185"
     CDS             70..1044
                     /locus_tag="VFM00_10185"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HET7498658.1"
                     /translation="MNKPSPASPRKPHPLSGHGDLLIGAHMSVAGGLLTGIERALKLR
                     IRAMQIFTKNASRWQADPLHEENALAFRNAWQASGLAPVAAHGSYLINLASPNPELRR
                     KSVLAYADELRRAESLGVRWLVTHPGAHMGSGEAAGCARIAESLDAARTLAPAPSVAT
                     LLETVAGQGTTLGRRFEELARIRSAVARPDLVQVCFDTCHVHAAGYDLVTERGCAATF
                     AEFNAVLGLESLRLFHVNDSKNERGSRVDRHEHLGRGRLGPEPFARILRDPRFHGTPK
                     VLETPKGKDGVVMDRRNLAFLRRLAGEPDAKGSNGRTGAEPPRGVGDS"
     gene            complement(1041..1304)
                     /locus_tag="VFM00_10190"
     CDS             complement(1041..1304)
                     /locus_tag="VFM00_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498659.1"
                     /translation="MSANDQDKDIVERLIAETNLSARMDALIGEICDKLREYGVASPH
                     KVFNDLVDPFREYFMLEYEEERGLTGSDEEEEEEDEEEEDRSY"
     gene            1496..3163
                     /gene="murJ"
                     /locus_tag="VFM00_10195"
     CDS             1496..3163
                     /gene="murJ"
                     /locus_tag="VFM00_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET7498660.1"
                     /translation="MTDASEPPPAPAAAAPAIPPASGAASGTRDRGMARAAGVISAAT
                     FLSRILGLVREQVFAAQFGAGFAVDAFQVAFRVPNLLRDLFAEGAMSAAFVPTLTRAE
                     KEGGTEAAMRLANLVVNFLLVVISALCLIGILAAPWIVKTMAPGFAAVPGKLELTTLM
                     TRIMTPFLLLVSLAAAVMGVLNTRRVFFLPAVAPTMLNLALILAGFIIAPYMPRFGLE
                     PIVGMAFGAVLGGLGQLLIQVPALRAHGYRWKPQISFRDPGVLRMVTLMAPASIGIAA
                     AQINIFTSTFLASMLVQGSVSWLNYAYRLMQLPIGLFGVAIATVTLAEVSRHAAAGNM
                     RDLKETLSFSLRLVLLLTLPATLLLIALARPIIALLYQHGRFDAADTVETARALWAYA
                     VGLAAFSAVRVMVPAFYSLGMARIPVMISMSTIGTTVLLYFPLMHFFQHTGLALAVSI
                     GSILNFTALFWMLRRKIGGLGGRKMLASGLRILAAAVGSAAAAAATARGLERLVGLHG
                     VAARVIVVGGALAVATGIYVGLCLLLRVEELKPLLGFASRLAGKRSA"
     gene            3160..3927
                     /locus_tag="VFM00_10200"
     CDS             3160..3927
                     /locus_tag="VFM00_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET7498661.1"
                     /translation="MTPRVVCLDVDGTLTDGVRGAVLPGAVEAVRRLRGRLPVRLVTN
                     TTSVPHAVLAETLMGQDLLDAPEHLWTPVLVARQELPARGHDAGILIADPWQRGEYSW
                     FREDAAGPAVLLATEAHEWTVGALQPAFRRLLEGAAFYALTRNRYFRKEGVLVTDVGG
                     VSALLAYTAGRPAETLGKPSRLLFEAVARHAGATLPEMVMVGDDAEFDVAMPIRLGLS
                     GILLRTGKYRVGDEARVDPRPTAVIASIADLPERLQL"
     gene            complement(3924..>4222)
                     /locus_tag="VFM00_10205"
     CDS             complement(3924..>4222)
                     /locus_tag="VFM00_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7498662.1"
                     /translation="ALSIANQERPRVVLLDIGLPGMDGYEVCRELRRTGHENALIVAL
                     TGYGQEEDRQRSRQAGFDGHLVKPGDPGELIKMVSAAPRLTRTAPESGLTPESA"
BASE COUNT          569 a         1512 c         1421 g          720 t
ORIGIN      
        1 tgttacttcg tcctgatccc ggcattctcc ccgtccctcg aacatcggcc gaatccgacc
       61 atttctacag tgaataaacc gagtcccgcc tccccccgaa aaccgcatcc cctttccggc
      121 catggtgatc tcctgatcgg agcccacatg agcgtggccg gcggcctgct gacgggaatc
      181 gagcgcgcgc tcaagctacg gattcgggcg atgcaaatct tcaccaaaaa cgcaagccgc
      241 tggcaggccg acccgctgca cgaagagaac gcgctcgcgt tccggaacgc ctggcaggcg
      301 agcgggctgg cgccggtcgc ggcgcacggc tcctatctca tcaacctggc ctctccaaat
      361 cccgagctcc gccggaagtc ggtcctggcc tatgcggacg agctgcggcg cgccgaatcg
      421 ctcggcgtgc gctggctggt cacccatccc ggggcccaca tgggtagcgg cgaggccgcg
      481 ggatgcgcgc ggatcgccga atcgctggac gccgcccgga ctctggcccc cgcgccctcg
      541 gtcgcgacgc ttctcgaaac ggtggcgggc caggggacga ccctcggccg gcgcttcgag
      601 gagctggccc ggatccggag cgccgtcgcg cgccccgacc tcgtccaggt ctgcttcgac
      661 acctgccacg tgcacgccgc cggctacgac ctggtcaccg aacgcggctg cgcggccacc
      721 ttcgccgaat tcaacgcagt gctgggattg gaatcgctgc gcctcttcca cgtcaacgat
      781 tcgaagaacg agcggggaag ccgggtggac cggcacgagc atctggggcg cggacggctg
      841 ggacccgagc cgttcgcgcg gatcctgcgc gatccgcgct ttcacgggac gccgaaagtg
      901 ctggagacgc ccaaggggaa ggatggcgtc gtgatggatc gaaggaatct ggcgttcctg
      961 cgccggctgg ctggagaacc ggacgcgaag ggatcgaatg gaagaaccgg agcggaaccg
     1021 ccgcggggag tcggcgattc ttagtagctc cggtcctctt cttcctcgtc ctcttcttct
     1081 tcctcttcgt cggagcccgt caggccgcgc tcttcttcat attccagcat gaagtattcg
     1141 cggaacggat cgacgagatc gttgaacacc ttgtggggcg aggcgacacc gtactcccgc
     1201 agcttgtcgc agatctcccc gatcagggca tccatgcgag ccgagagatt ggtctccgcg
     1261 atgagacgtt cgacgatgtc cttgtcctgg tcgttcgcgc tcatggctgt cgcccccttg
     1321 tttcgagaat ggggtcaccg gggagcatgg aaggtggtcc cggatcggcc tcgattatat
     1381 caaaccctgt gccgtcgcgc aggttctcga atcggctccc caggccgatt tttcgggtgc
     1441 cgcctaaccc cttcccgctt gacagggtag cgggcagccc ggagcatccg acgccgtgac
     1501 cgacgcctcc gagccgcctc ccgcccccgc cgccgcagca ccggccattc cccccgcgtc
     1561 cggtgccgct tccggcaccc gggaccgcgg catggcccgc gcggccgggg tgatctccgc
     1621 cgccaccttc ctgagccgca tcctgggcct ggtgcgcgag caggtcttcg cggcgcagtt
     1681 cggggccggg ttcgcggtgg acgccttcca ggtggccttc cgggtgccca acctcctccg
     1741 ggacctcttt gccgaggggg cgatgagcgc cgccttcgtc cccaccctga cccgcgcgga
     1801 gaaggagggg ggtaccgagg ccgcgatgcg gctcgcgaac ctggtcgtca acttcctcct
     1861 ggtcgtcatc tccgccctct gcctgatcgg gatcctggcg gccccctgga tcgtgaagac
     1921 gatggcgccg ggcttcgcgg ccgtgcccgg gaagctcgag ctgaccaccc tcatgacccg
     1981 gatcatgacc ccgttcctgc tgctcgtctc gctggccgcg gcggtgatgg gcgtgctgaa
     2041 cacgcgcagg gtcttcttcc tcccggccgt ggcgccgacg atgctgaacc tggcgctgat
     2101 cctggccggc ttcatcatcg ccccctacat gccgcgcttc ggcctggagc cgatcgtcgg
     2161 gatggcgttc ggcgccgtgc tgggcgggtt ggggcaactg ctgatccagg tgcccgcgct
     2221 gcgggcgcac ggataccggt ggaagccgca gatctccttc cgcgaccccg gcgtgctccg
     2281 catggtgacc ctgatggccc ccgcttccat cgggatcgcg gccgcccaga tcaacatctt
     2341 cacgagcacc ttcctcgcct cgatgctggt ccagggaagc gtctcctggc tcaactacgc
     2401 ctaccgtctc atgcagctcc cgatcggcct cttcggcgtg gcgatcgcca cggtcacgct
     2461 ggccgaggtc tcgcggcacg ccgcggccgg gaacatgcgc gacctgaaag agaccctctc
     2521 cttctcgctc cggctggtgc tcctgctcac gctgcccgcg acgctcctcc tgatcgcgct
     2581 cgcgcggccg atcatcgcac tcctctacca gcacggccgc ttcgacgccg cggacaccgt
     2641 cgagaccgcg cgcgccctct gggcctacgc ggtggggctg gccgccttct ccgccgtgcg
     2701 cgtgatggtg ccggccttct attcgctggg catggcgcgc atcccggtca tgatctccat
     2761 gagcacgatc ggcacgacgg tgctcctcta cttcccgctc atgcacttct tccagcacac
     2821 ggggctcgcg ctggcggtgt cgatcggctc gatcctcaac ttcaccgccc tcttctggat
     2881 gctgcggcgg aagatcggcg ggctgggcgg gcggaagatg ctggcctcgg gcctgcgcat
     2941 cctggccgcg gccgtcggct cggccgcggc ggccgccgcg acggcgcgcg gcctggagag
     3001 gctcgtggga ctgcacggcg tggcggcgcg agtgatcgtc gtgggcggcg cgctggcggt
     3061 ggcgaccggg atctacgtgg gactctgcct cctccttcgg gtcgaggaat tgaagccgct
     3121 cctgggcttc gcgtcgcggc tggccggcaa gcggagcgcg tgacgccccg ggtcgtctgc
     3181 ctggacgtgg acggcacgct caccgacggc gtgcggggag ccgtgcttcc cggagcggtc
     3241 gaggcggtgc gccggctgcg agggcgcctc ccggtgcggc tggtcacgaa cacgaccagc
     3301 gtgccccacg ccgtgctggc cgagaccctc atgggccagg atctcctcga tgcacccgag
     3361 catctctgga ctccggttct cgtcgcccgc caggagctcc cggcacgcgg ccatgacgcg
     3421 gggatcctga tcgccgatcc ctggcagcga ggggagtact cctggttccg tgaggacgcc
     3481 gccggtcccg ccgtgctcct cgccaccgag gcgcacgaat ggaccgtggg cgcgctccag
     3541 ccggcgttcc gccgcctgct cgagggagcc gcgttctatg ccctcacccg gaaccggtat
     3601 ttccggaagg agggcgtact cgtgacggac gtcgggggcg tgtcggcgct gctcgcctac
     3661 accgccggcc gtccggcgga gacgctgggc aagccctcgc ggctcctgtt cgaggcggtg
     3721 gcccgccacg cgggcgcgac cctgcccgag atggtcatgg tgggcgacga tgccgagttc
     3781 gacgtggcga tgcccatccg gctgggtctc tcgggcattc tgctgcgcac cgggaaatac
     3841 cgcgtgggcg acgaggcgcg cgtcgacccg cgaccgacag ccgtgatcgc ctcgatcgcc
     3901 gatctgccgg agcggctcca gctctaggcc gactcggggg tgaggcccga ctcgggggct
     3961 gtccgcgtga ggcgcggcgc cgccgagacc atcttgatca gctcgccggg gtcgccgggc
     4021 ttgaccaagt ggccgtcgaa gccggcctgg cgcgaacgct gccggtcctc ctcctgcccg
     4081 taccccgtga gcgccacgat caaggcgttc tcgtgcccgg tgcgccggag ctcgcggcag
     4141 acttcgtagc cgtccattcc cggaagtccg atgtccagaa gcacgacgcg gggccgctcc
     4201 tggttcgcga tggagagcgc gg
//
LOCUS       DASSLZ010000271        10166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_355753, whole genome shotgun sequence.
ACCESSION   DASSLZ010000271 DASSLZ010000000
VERSION     DASSLZ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355753"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFM00_10210"
     CDS             complement(<1..484)
                     /locus_tag="VFM00_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498663.1"
                     /translation="MRATVRNLPRAKRPERRHGDGARAGRFRRLIFTMRPEEVMTMIL
                     FVPVAVALAGVSANLAAYPVDPAAAGYPSAAARLLALVATVALTLWVVRTKPRWELAR
                     DSLPFLFCGSIYASLHDLIRFYGFPDITRALYRGDVALFGVEPTVWAQQFTAPFWTDF
                     L"
     gene            complement(481..2652)
                     /locus_tag="VFM00_10215"
     CDS             complement(481..2652)
                     /locus_tag="VFM00_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET7498664.1"
                     /translation="MGLSLKPLLCPRSIAVIGASRRRDSIGGAVLHNLLQRPFQGPVY
                     PVNPNATHIQSVPAYASIEDVPGDVDLAIIVVPAAAVLEAAEACGKKGVKALVVISAG
                     FKEIGGAGIALEQELRALVRRHGMRLVGPNCLGILNTDPNVNMNATFAPVQPPPGRIA
                     FSSQSGALGLAILDYARALNLGISHFVSVGNKADVSGNDLLEFWEQDPGTDLVLFYLE
                     SFGNPGKFTQIARRVARTKPIVAVKSGRTVSGSRAASSHTGALAGSDAAVEALFRQSG
                     VIRTDTIEELFDTALLLGSQPIPTGPRVAILTNAGGPGIMAADACESAGLQLPALDAK
                     TVKGLKAFLPPEASTRNPVDMIASAVAESFEKALRLLVADKNVDAVIVIFVPPLVTGA
                     QDVARAILAGAAGSPKPVLSCFMGSHGVPESLVSLNEGHIPSYSFPEAAARTLARVAR
                     YGQWRRARAGVVPTLSGVDRAQARAAIEASDAGTWMSAPLLADLLQAYGIRTNPLRVA
                     ATRGEAAVMAKAVGFPAALKLRSPDVVHKSDVGGVKLRIQSEEDAARAFDDIAKALRN
                     HDPKARFEGVTVERMVDGGVETIVGMTRDPSFGPVVLFGLGGVTVELLRDVSLRVAPL
                     TDTDADEMLREIRGFQLLEGYRGAPPVNQASLRDVLHRVSLLALENPEIGELDLNPVL
                     AFPGEAPAVALDARLKLAPATAAGEAGPKAEERAPAPAGTA"
     gene            complement(2821..3126)
                     /locus_tag="VFM00_10220"
     CDS             complement(2821..3126)
                     /locus_tag="VFM00_10220"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007532552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET7498665.1"
                     /translation="MPTAKLSDAGITAALAGLPGWKLEAGMLQKTYKHETFPEAIVFV
                     NAVAHLAELANHHPDVDIRYSNITLRLTTHDSGGITAKDVELAKEVEAVRKKMGVPT"
     gene            complement(3166..3453)
                     /locus_tag="VFM00_10225"
     CDS             complement(3166..3453)
                     /locus_tag="VFM00_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET7498666.1"
                     /translation="MASDEEIRTALEEVVDPEIHLSIIDLGLVREIDQSVEPHVVRML
                     LTTPFCPYAPQIVAQVKEVTTRVTGKETEVEILPDPWSPEMMSDPGLLGRW"
     gene            complement(3466..3795)
                     /locus_tag="VFM00_10230"
     CDS             complement(3466..3795)
                     /locus_tag="VFM00_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET7498667.1"
                     /translation="MIEKAARFVKVGKVADIPEGRPETFEVEDRHIAIYRLNDGYYAI
                     DDICTHDGGPLAEGEVDDDVVICPRHGARFSIRTGAALSFPAVTSVDTYPVRVEGDDL
                     LVGLPED"
     gene            complement(3818..4216)
                     /locus_tag="VFM00_10235"
     CDS             complement(3818..4216)
                     /locus_tag="VFM00_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET7498668.1"
                     /translation="MSLDDLYRQDILDHSQNPRNFGTLEHPEISAEDSNPLCGDKIRM
                     DLRVKDGTIEDVKFTGVGCSISRAAASMLTEEIKGKTLEEVKRIGRDDVLELLGIELG
                     PVRLKCALLALKTLKVGIYGLQSWKDEEEE"
     gene            complement(4221..5477)
                     /locus_tag="VFM00_10240"
     CDS             complement(4221..5477)
                     /locus_tag="VFM00_10240"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET7498669.1"
                     /translation="MRGGAAVAPERRLDVAAIRKDFPILAREIYGKRLAYLDNTATTQ
                     KPIQVLRAIEEFYRTSNANVHRGVYLMAEESTEKYEAARGIVARFLNARSPKEIVFTR
                     GTTESINLVSNSWGRKNVGPGDVIVATEMEHHSNMVPWQLLAEEKGATLRYIPVDGSG
                     RLDLSNLDAILDGPVKLLAVAHMANAIGTINPVDELIRRAHAAGAVVLVDGAQSVPHL
                     GTDVRALDCDFLAFSGHKMLAPTGIGALYAKRDLLEAMPPFFGGGEMIREVKLSGSTW
                     NEIPFKFEAGTMNIGDAIALGVAIEYLEGLGMDAVRRHDAELTVQALEGLGAIPGVTI
                     YGPPAPERGGIVSFTVEGIHAHDVAAVLDREGIAVRAGHHCTMPLHAKFGIAASTRAS
                     FYVYNLPEEIDRLVAGVRKAKQVFNS"
     gene            complement(5510..6880)
                     /gene="sufD"
                     /locus_tag="VFM00_10245"
     CDS             complement(5510..6880)
                     /gene="sufD"
                     /locus_tag="VFM00_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET7498670.1"
                     /translation="MARTENPATTLEPASALPGRLGGLTDAGVSALAARAEEPAFLAE
                     RRARAWAFSQKTPFPTRLEELWRRTDISGLKWDALLIDRESHPVARSVADLPAELRAE
                     IGPADERSALVVVLDADVVLLDRDGSLARAGVTVLSIAQAAREKPELVQRLVGASVRY
                     DESRFTALNAALRSEGVLIHVPAGAVVEKPIRVLIARGTPDLAIFPHLVVALEKGAKA
                     TVIEEYVSSGEPGVAVVVPVTEITVGEAADLHLATLNHWAGNVYHFGVERARIGRDAR
                     FHWTFAALGGKLVKSDMELHLEGEGAEGKFSGCYFGNESQHFDFHTFQNHVVGNTTSD
                     LLFKGALRGRARTIYQGLIRVHKNAQRSDAYQANRNLILSDKARADSIPSLEIEANDV
                     RCTHGATVGQVDDEQLFYLMARGLAKPDAERLIISGFFEPVLERIPSPTLRALVTEAM
                     DQKAGR"
     gene            complement(6925..8331)
                     /gene="sufB"
                     /locus_tag="VFM00_10250"
     CDS             complement(6925..8331)
                     /gene="sufB"
                     /locus_tag="VFM00_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET7498671.1"
                     /translation="MTEQSLDIREGYKEKYGFHDDIAPVFKSRRGLDKDVVSEISAMK
                     GEPAWMRDFRLKALEQFEKKPLPNWGGNVGEIDFQNIFYYLKPTDEQGKNWDDVPADI
                     KRTFDKLGIPEAEQKFLSGVGAQYDSETVYHKIKEDLANKGVIFLDTGSALEQHPELF
                     KQYFGTIIPPSDNKFAALNSAVWSGGSFIYVPKGVKVDLPLQAYFRINAQNMGQFERT
                     LIIVDEGAFVHYVEGCTAPIYASDSLHAAVVEIIVKKGARCRYTTIQNWSNNVFNLVT
                     KRAAAYQDATMEWVDANLGSKLTMNYPSVYMMEPGAHAEILSIAFAGKGQHQDAGGKC
                     VHAASNTTSNVVSKSISKDGGRAGYRGLVKVAKGAINCKSTVRCDALLLDEDSRSDTY
                     PYMEIEEDKVSIGHEATVSKIGDEQLFYLMSRGIPEAEAAGMIVSGFIEPITKELPME
                     YAVEMNRLIQLQMEGSVG"
     gene            complement(8357..9130)
                     /gene="sufC"
                     /locus_tag="VFM00_10255"
     CDS             complement(8357..9130)
                     /gene="sufC"
                     /locus_tag="VFM00_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET7498672.1"
                     /translation="MKSGTPTLQIDNLHVAVEGKPILKGLTLTVNQGEIHAIMGPNGS
                     GKSTLANALMGHPKYQVTGGSVLFKGQDIAEWSADERGRRGLFLAVQYPTAIPGVSVA
                     NFLRMSLQARRKELGEEKPLPPKEFRALVKEKMALLKMDDAFAGRYINDGFSGGEKKR
                     AEILQMAVLRPEIAVLDETDSGLDIDALRIVAEGVNALAGPDLGVLLITHYQRILNYI
                     KPQFVHVLVDGRIVKSGGAELAHELEAQGYIGVGDPQAA"
     gene            complement(9213..9590)
                     /locus_tag="VFM00_10260"
     CDS             complement(9213..9590)
                     /locus_tag="VFM00_10260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET7498673.1"
                     /translation="MSETTAENRADSTGAPKGTISLTPAAIEWVLRVRTKEGKEGQDL
                     RLGVKAGGCSGYSYFMGFTTARRPNDLVLDYEGITVLVDPRSLEILDGTVVDYQRGML
                     GTGIQFKNPRVKKSCGCGDSFSL"
     gene            complement(9587..>10166)
                     /locus_tag="VFM00_10265"
     CDS             complement(9587..>10166)
                     /locus_tag="VFM00_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7498674.1"
                     /translation="IRKGMMPSKADPGDSMDHHDSSKLLKVGDMARRTGKSVRALRLY
                     EELGLLTPTARSIGGFRLYDEGALTRIRWIELLQESGFTLHQIQDLLKAWQATHYGPD
                     AMKVVRETFEKRLAETRAALERFAALEKELEGSLQYLNTCETCRPPRTTQEDCPQCPE
                     DHGMQEPPALVAGFQVGARTGITLRVLTTESA"
BASE COUNT         1509 a         3498 c         3396 g         1763 t
ORIGIN      
        1 tgaggaaatc ggtccagaac ggcgccgtga actgctgcgc ccacacggtg ggctcgacgc
       61 cgaagagcgc cacgtccccg cggtagagcg cccgggtgat gtcgggaaag ccgtagaagc
      121 ggatcaggtc gtgaagactc gcgtagatgc tgccgcagaa gagaaacggc agggagtcgc
      181 gcgcgagctc ccagcgcggc ttggtccgca cgacccacag cgtgagcgcc accgtcgcga
      241 ccagcgcgag cagccgcgcg gcggcgctcg ggtagcccgc cgccgccggg tcgaccggat
      301 acgccgcgag gttcgcggac acgccggcca gcgcgaccgc gaccggaacg aagaggatca
      361 tcgtcatcac ctcttcgggc cgcatcgtga agatcaggcg ccggaaccgc ccggcccgcg
      421 ccccgtcgcc atggcggcgc tccggccgct tggcgcgcgg caggttccgg acggtggccc
      481 tcacgccgtg cccgccggtg ccggcgcgcg ctcctccgct ttcggtcccg cctcgccggc
      541 ggccgtggcc ggggccagct tgagccgcgc atccagcgcc accgcgggcg cctcgcccgg
      601 aaaggcgagc accgggttca agtccagctc tccgatctcg gggttctcca gggcgagcag
      661 cgagacccgg tgcaggacat cccgcagcga ggcctggttc acgggcggag cgccgcgata
      721 gccctcgagc agctggaagc cgcggatctc gcgcagcatc tcatcggcgt cggtgtcggt
      781 cagcggggcc acccgcagcg acacgtcgcg cagcagctcg accgtgacgc cgcccaggcc
      841 gaacaggacg acggggccga aggaggggtc gcgcgtcatg ccgacgatcg tctccacgcc
      901 gccgtccacc atccgctcca cggtgacgcc ttcgaagcgc gccttggggt cgtggttgcg
      961 aagcgccttg gcgatgtcgt cgaacgcgcg ggccgcgtct tcctcgctct ggatccggag
     1021 cttcacgccc cccacgtcgc tcttgtggac cacgtccggc gagcggagct tgagcgccgc
     1081 cgggaagccg accgccttcg ccatcaccgc ggcctcgccg cgcgtcgcgg cgacgcggag
     1141 cgggttcgtg cggatcccgt aggcctggag caggtcggcc aggaggggcg cggacatcca
     1201 ggtgccggcg tcggacgcct cgatcgcggc gcgcgcctgg gcgcgatcca cccccgagag
     1261 cgtgggcacg actccggccc gcgcgcggcg ccactgcccg tagcgtgcga cgcgcgccag
     1321 cgtgcgcgcc gccgcctcgg ggaagctgta cgacgggatg tggccttcgt tcaacgagac
     1381 gaggctctcc ggcacgccgt gggatcccat gaagcaggag agcaccggct tcgggcttcc
     1441 cgccgctccc gcgaggatcg cgcgggccac gtcctgcgcg ccggtcacga ggggcgggac
     1501 gaagatcacg atcaccgcgt ccacgttctt gtccgccacc agaagccgga gcgctttctc
     1561 gaacgactcg gcgacggccg aagcgatcat gtcgaccggg tttcgcgtgc tcgcctcggg
     1621 aggcaggaac gccttcagcc ccttcaccgt tttggcatcg agcgcgggaa gctggaggcc
     1681 cgcgctctcg caggcgtcgg cggccatgat ccccggaccg cccgcgttcg tgaggatggc
     1741 cacgcgcggt cccgtcggga tcggctgcga gcccaggagg agtgccgtgt cgaacagctc
     1801 ctcgatcgta tccgtgcgga tcacgccgct ctggcggaag agcgcctcga cggcggcgtc
     1861 cgaccccgcg agcgcgccgg tgtgcgacga cgcggcgcgc gatccgctga cggtgcgtcc
     1921 gctcttcacg gcgacgatcg gcttggtgcg cgcgacacgt cgggcgatct gcgtgaactt
     1981 gccgggattg ccgaacgact ccagatagaa gaggacgagg tcggtgccgg gatcctgctc
     2041 ccagaattcg agcaggtcgt tccccgagac gtcggccttg ttccccacgc tcacgaagtg
     2101 ggagatgccc aggttcagcg cgcgcgcgta gtcgaggatc gcgagcccca aggcaccgct
     2161 ctgcgacgag aaagcgatcc gccccggcgg cggctgcacc ggcgcgaagg tggcgttcat
     2221 gttcacgttc ggatcggtgt tcaggatccc caggcagttc ggccccacga gccgcatgcc
     2281 gtggcgccgc accagcgcgc gcagctcctg ttcgagcgcg atccccgcgc cgccgatctc
     2341 cttgaagccc gcggagatca cgaccagcgc ctttaccccc ttcttcccgc acgcctccgc
     2401 cgcctcgagc accgcggccg cggggaccac gatgatcgcg aggtccacgt cgcccggaac
     2461 gtcctcgatc gacgcgtagg ccggtaccga ctggatatgc gtggcgttgg gattgacggg
     2521 atagaccggc ccctggaagg gccgctgcag caggttatgg agtaccgccc cgccgatcga
     2581 atcgcgccgg cgcgaagcgc cgatcacggc gatggagcgg ggacacagaa gtggcttgag
     2641 tgagagtccc atgggtcggg attctagcag atcggagcgc gcgtcttgcc gggctcaggc
     2701 atgcggccat cgtcaacgcc tgttaccctc gccagcggcg gtaacgtccg ctgacgcggg
     2761 gttgacataa tcacttgcgc tggcgcaagt ttatatttat gttaattccc cgcggggata
     2821 ttacgtcggg acgcccatct tcttccgcac ggcctccact tccttcgcca gctccacgtc
     2881 cttcgcggtg attcccccgg aatcgtgcgt cgtgaggcgc agcgtgatgt tcgagtagcg
     2941 gatgtcgacg tcggggtggt ggttcgccag ctcggccaga tgggcgacgg cgttcacgaa
     3001 cacgatcgcc tcggggaagg tctcgtgctt gtaggttttc tggagcatgc cggcctcgag
     3061 cttccagccg ggcagccccg cgagcgcggc cgtgatgccg gcgtcgctca gctttgcggt
     3121 gggcatcgtc tcaactcctc gcggccccat ttcggggact gccgactacc accggccgag
     3181 aagacctgga tcggacatca tttccggaga ccagggatcc gggaggatct ccacctcggt
     3241 ctccttcccg gtcacgcgcg tggtcacttc cttcacctgc gcgacgatct gcggcgcgta
     3301 ggggcagaag ggcgtcgtga gcagcatgcg cacaacgtgc ggctcgacgc tctggtcgat
     3361 ctcgcggacc aggcccaggt cgatgatgct caggtggatc tcgggatcca cgacttcctc
     3421 cagcgcggtg cggatttctt cgtcgcttgc catatcgagt cctctctagt cttcgggaag
     3481 tccgaccagc aggtcgtccc cttccacccg caccgggtag gtgtcgaccg acgttacggc
     3541 tgggaacgaa agcgcagcac cggtccgaat gctgaatcgc gcaccgtggc gcgggcagat
     3601 cacgacgtcg tcgtcgacct ccccttcggc cagcggcccg ccgtcatggg tgcagatgtc
     3661 gtcgatcgcg tagtagccgt cgttcaggcg gtagatggcg atgtgccgat cctccacctc
     3721 gaacgtctcg gggcggcctt ccgggatgtc cgcgaccttc cccaccttca cgaaccgagc
     3781 cgccttctcg atcacgatcg ctccctcccg cacacgctca ctcctcctcc tcgtccttcc
     3841 agctctgcag tccgtagatc cccaccttga gcgtcttcag ggcgaggagc gcgcacttga
     3901 gccgcaccgg acccagctcg atgcccagga gctcgagcac gtcgtcgcgc ccgatccgct
     3961 tcacctcttc cagcgtcttc cccttgatct cctcggtcag catcgaggcc gccgcccggc
     4021 tgatcgagca gccgacgccg gtgaacttca cgtcctcgat ggtaccgtcc ttcacccgga
     4081 gatccatgcg gatcttgtcc ccgcagagcg gattggaatc ctccgcgctg atctccggat
     4141 gctcgagggt gccgaaattc cggggattct gggagtggtc gaggatgtcc tgccggtaga
     4201 ggtcgtccag gctcatgggt tcaggagttg aagacctgct tcgccttgcg gacgccggcg
     4261 accagccggt cgatctcttc gggaaggttg tagacgtaga aggaggcgcg cgtggaggcg
     4321 gcgatcccga acttggcgtg gagcggcatc gtacagtggt gccccgcgcg caccgcgatc
     4381 ccctcgcggt cgagcacggc cgccacgtcg tgggcgtgaa tcccctccac ggtgaaggag
     4441 acgatcccgc cccgctcggg cgcgggcggg ccgtagatcg tcacgccggg gatcgcgccc
     4501 agcccttcga gcgcctggac cgtgagctcc gcgtcgtggc gacgaacggc gtccataccc
     4561 agcccttcga gatattcgat cgccacgccc agcgcgatcg cgtcgccgat gttcatggtg
     4621 cccgcctcga acttgaacgg aatctcgttc caggtcgagc cggacagctt gacctcgcgg
     4681 atcatctcgc cgcccccgaa gaaggggggc atcgcctcga gaaggtcgcg cttggcgtac
     4741 aacgccccga tgccggtcgg cgcgagcatc ttgtggccgg agaaagccag gaagtcgcag
     4801 tcgagcgcgc gcacgtcggt gcccaggtgc ggcacgctct gggcgccgtc cacgagcacg
     4861 acagcgcccg cggcgtgtgc ccgtcggatc agctcgtcga cgggattgat cgttccgatc
     4921 gcgttcgcca tgtgcgcgac ggccaggagc ttcacggggc cgtccaggat cgcgtccagg
     4981 ttggagaggt ccagccgtcc gctcccgtcc acggggatgt agcggagcgt ggcgcccttc
     5041 tcctcggcca acagctgcca gggcaccatg ttcgagtggt gctccatctc ggtggcgacg
     5101 atgacgtcgc cggggccgac gttcttccgt ccccaggagt tcgagacgag attgatcgat
     5161 tcggtcgtgc cgcgcgtgaa gacgatctcc ttgggcgagc gcgcgttcaa gaaccgcgcg
     5221 acgatgccgc gcgccgcctc gtacttctcg gtggactcct cggccatgag gtagacgccg
     5281 cggtgcacgt tggcgttgct ggtgcgatag aactcctcga tcgcgcgcag cacctggatc
     5341 ggcttttggg tcgtggccgt gttgtcgagg taggcgagcc gcttgccgta gatctcgcgc
     5401 gcgaggatcg ggaagtcctt ccggatcgcg gcgacgtcca gccggcgctc gggcgccacc
     5461 gcggcgccgc ccctcatcgc tccggctcct tccgccgcgc gcgtcatcgt caccgtcccg
     5521 ccttctggtc catcgcctcg gtcaccaggg cccgcaacgt cggcgaggga atccgctcca
     5581 gcaccggctc gaagaagccg gagatgatca gccgctcggc gtccggcttg gccagcccgc
     5641 gcgccatgag atagaagagc tgctcgtcgt ccacctgccc cacggtggct ccgtgcgtgc
     5701 agcgcacgtc gttcgcctcg atctcgaggc tgggaatgga gtcggcgcgc gccttgtcgg
     5761 agaggatcag gttccggttc gcctggtagg cgtcggagcg ctgggcgttc ttgtgcacgc
     5821 ggatcagccc ctgatagatc gtgcgggccc gtccgcgcag cgcccccttg aagagcagat
     5881 cgctggtcgt gttccccacc acgtggttct ggaacgtgtg gaaatcgaaa tgctgcgact
     5941 cgttgccgaa gtagcagccg gagaacttac cctcggcgcc ctccccctcc aggtgcagct
     6001 ccatgtccga cttgaccagc ttcccgccga gtgccgcgaa agtccagtgg aagcgggcat
     6061 cgcgcccgat gcgcgcgcgc tcgacgccga agtgatagac gttccccgcc cagtggttca
     6121 gcgtggccag gtgaaggtcg gcggcctcgc ccaccgtgat ctcggtgacc ggcaccacga
     6181 ccgccacgcc cggctcgccc gaggagacgt actcctcgat gacggtcgcc ttggcgccct
     6241 tctccagcgc gacgaccagg tgcggaaaga tcgcgaggtc gggcgttccc cgcgcgatca
     6301 gcacgcggat cggcttctcg acgaccgcgc ccgcgggaac gtggatcagc acgccctcgg
     6361 agcgtagcgc ggcgttcaaa gcggtgaagc ggctctcgtc gtagcgcacc gaagcgccca
     6421 ccagccgctg gaccagctcc ggcttctccc gcgcggcctg cgcgatggag agcaccgtga
     6481 cccccgcgcg cgccagcgag ccgtcgcgat cgaggagcac gacgtcggcg tccagcacga
     6541 ccacgagcgc cgagcgctcg tcggcgggcc cgatctcggc gcgcagctcg gccggcagat
     6601 cggccacgga gcgcgcgacc ggatgcgatt cccggtcgat caggagcgcg tcccacttga
     6661 ggccggagat gtcggtccgg cgccacagct cctcgagccg cgtcgggaag ggcgtcttct
     6721 ggctgaaggc ccaggcgcgg gcgcggcgct cggcgagaaa cgccggctcc tccgcgcgcg
     6781 cggcgagggc ggagacgccc gcgtcggtca ggccgccgag ccggccgggg agggccgacg
     6841 cgggctcgag cgtggtggct gggttttcgg tacgggccac gttttctcct ctcatgtcta
     6901 tggcgtttcg gggcgggccg gcgatcagcc gaccgagccc tccatctgaa gctgaatcag
     6961 gcggttcatc tccacggcgt actccatcgg cagctccttc gtgatcggct cgatgaatcc
     7021 gctcacgatc atcccggccg cttccgcctc gggaatgccg cggctcatga gatagaagag
     7081 ctgctcgtcg ccgatcttcg agacggtcgc ctcgtggccg atcgagacct tgtcttcctc
     7141 gatctccatg tagggatagg tgtcggagcg cgagtcctcg tccaggagga gcgcgtcgca
     7201 gcgcaccgtc gacttgcagt tgatcgcgcc cttcgccacc ttgaccaggc cgcgataccc
     7261 cgcgcgcccg ccgtccttgc tgatcgactt ggagacgacg ttcgacgtgg tgttggaggc
     7321 cgcgtgcacg cacttgccgc cggcgtcctg gtgctgcccc ttgccggcga aggcgatcga
     7381 caggatctcg gcgtgcgcgc ccggctccat catgtagacg ctcgggtagt tcatcgtgag
     7441 cttggagcca aggttcgcgt ccacccactc catcgtggcg tcctggtagg ccgccgcgcg
     7501 cttggtgacc aggttgaaca cgttgttcga ccagttctgg atggtggtgt agcggcagcg
     7561 cgcgcccttc ttgacgatga tctcgaccac cgcggcgtgc agcgagtcgc tcgcgtagat
     7621 cggcgcggtg cagccctcga cgtagtgcac gaaggcgccc tcgtccacga tgatcagcgt
     7681 ccgctcgaac tgccccatgt tctgggcgtt gatccggaaa taggcctgca ggggcaggtc
     7741 gaccttcacc cccttgggca cgtagatgaa gctgccgccg ctccagaccg cgctgttcag
     7801 cgcggcgaac ttgttgtccg agggcggaat gatcgtgccg aagtactgct tgaagagctc
     7861 ggggtgctgc tccaaggccg agcccgtgtc gaggaagatg acccccttgt tcgcgaggtc
     7921 ctccttgatc ttgtggtaga cggtctcgga gtcgtactgg gcgcccacgc cggagaggaa
     7981 cttctgctcc gcctcgggga tgcccagctt gtcgaacgtg cgcttgatgt ccgcgggcac
     8041 gtcgtcccag ttcttcccct gctcgtcggt cggcttcagg tagtagaaga tgttctggaa
     8101 gtcgatctcc ccgacgttgc cgccccagtt ggggagcggc ttcttctcga actgctcgag
     8161 ggccttcagg cggaagtcgc gcatccaggc cggctccccc ttcatcgccg agatctcgga
     8221 gaccacgtcc ttgtccagcc cgcggcggga cttgaagacg ggggcgatgt cgtcgtggaa
     8281 gccgtacttc tccttgtagc cttcgcgaat gtcgagcgac tgttcggtca tcacggctcc
     8341 tttatcgtcc gaggcgctac gcggcctggg gatcgcccac gccgatgtat ccctgcgcct
     8401 ccagctcgtg cgccagctcc gccccgccgg acttgacgat ccggccgtcg acgagcacat
     8461 ggacgaactg cggcttgatg tagttgagaa tgcgctggta gtgcgtgatc aacagcacgc
     8521 ccaggtcggg tccggccagc gcgttgaccc cttccgccac gatgcggagc gcgtcgatgt
     8581 cgagaccgga atcggtctcg tccaggaccg cgatctccgg ccggagcacc gccatctgga
     8641 ggatctcggc gcgcttcttc tccccgcccg agaagccgtc gttgatgtag cggcccgcga
     8701 aggcgtcgtc catcttgaga agcgccatct tctccttcac cagcgcgcgg aattccttgg
     8761 gcggaagcgg cttctcctcg cccaactcct tgcgccgcgc ctgcagcgac atgcgcagga
     8821 agttcgccac gctgacgccc ggaatcgcgg tcgggtactg gaccgcgagg aagagcccgc
     8881 ggcgcccacg ctcgtcggcg ctccactcgg cgatgtcctg ccccttgaag aggaccgagc
     8941 cgccggtcac ctggtacttc gggtggccca tgagggcgtt ggcgagggtg ctcttgcccg
     9001 agccgttcgg tcccatgatg gcgtggatct cgccctggtt cacggtgagg gtcagcccct
     9061 tgaggatcgg cttcccttcc accgcgacgt ggagattgtc gatctgaagc gtcggcgtgc
     9121 cgctcttcat gtgctccctt tcttcgatgg ttcgtgcgcg ccgcgcgcgc ggtggccggg
     9181 cccgatcccc ggttcgggga ccgcggagcc cgctacaggc tgaagctgtc gccgcacccg
     9241 cacgacttct tcacgcgcgg gttcttgaac tggatgcccg tcccgagcat gccccgctgg
     9301 tagtccacga cggttccgtc gaggatctcc agcgagcgcg ggtccacgag caccgtgatc
     9361 ccctcgtagt ccagcacgag gtcgttcggc cggcgcgccg tggtgaagcc catgaaatag
     9421 gaatagccgg agcaaccgcc cgccttgacg cccaggcgga ggtcctgccc ttccttgcct
     9481 tccttggtcc ggacccggag cacccactcg atggcggcgg gggtcaggct gatcgttccc
     9541 ttcggagcgc cggtggaatc ggcgcggttt tcggcggtcg tctcgctcat gcgctctccg
     9601 tcgtcagcac ccgcagggtg atccccgtgc gagcccccac ctggaatccc gcgaccaggg
     9661 ccggcggctc ctgcatgccg tgatcctcgg ggcactgcgg gcaatcctcc tgcgtggtcc
     9721 gcggcgggcg gcacgtctcg caggtgttca ggtactggag cgatccctcc agctcctttt
     9781 cgagcgccgc gaagcgctcg agcgcggccc gcgtttccgc gagccgcttc tcgaaggtct
     9841 cgcgcaccac tttcatcgcg tcgggaccgt aatgggtggc ttgccaagct ttcagaagat
     9901 cttgaatctg atgaagcgtg aatccgcttt cctgaagcag ctcgatccac cggatgcgcg
     9961 tgagcgcgcc ctcgtcgtag agccgaaagc cgccgatcga tcgggctgtc ggagtcagca
    10021 ggcccagttc ctcatagagc cgcagcgcgc ggacggactt tcccgtgcgc cgagccatgt
    10081 cgccaacttt gagcagcttg gaggaatcgt ggtggtccat ggaatctccg ggatcggctt
    10141 tcgagggcat cataccctta cgtata
//
LOCUS       DASSLZ010000272        16562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1727239, whole genome shotgun sequence.
ACCESSION   DASSLZ010000272 DASSLZ010000000
VERSION     DASSLZ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 16562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16562
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727239"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1214)
                     /locus_tag="VFM00_10270"
     CDS             complement(141..1214)
                     /locus_tag="VFM00_10270"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET7498675.1"
                     /translation="MTDGEREEIRRAIDRLDRDLLRLLEDRAELARRIGALKQASGEA
                     TLDAARERDLLERIEREAKGAFPRSGVRAIFREIVSASRAAQGAFRVAYLGAEGGFAH
                     QAAVRRFGASSTYAATASPQHLLEAIGSERAEHGVFALEGDAEDPPFDAYDLLLGAEA
                     AITAEFVDRGGYEALGRARTPKRLIAHPAALALCQRWRAALGPGVEVVAAAGSLEAGR
                     RAAADPDAFCLAPAIVADLAGLPEVEPEAEDAPRRTRRFLVLGRSAGAASGRDKTALL
                     LALENTPGALLEALRALAAHGVNLLWIESRTHRWRPGEHLFLLEVAGHQDADPLRSAL
                     AEMRARTRLFRVLGSFPAAESGW"
     gene            complement(1211..1939)
                     /locus_tag="VFM00_10275"
     CDS             complement(1211..1939)
                     /locus_tag="VFM00_10275"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET7498676.1"
                     /translation="MRLNLFLARAGGGSRREADQWIREGRVTVNGLPPHGMGAPVVPG
                     RDQVALDGAVLLLPDAERYAAYHKPPGVLVSRRSQGGKPTIFDRLGEEARGMMPVGRL
                     DYQSEGLLLLTTDGALAEALLHPRSALIRSYRVWVRPVPDPAIVRLLREGAVVEGVPV
                     RPAAVVLEGAERGRGIVRFDLAEGKKREVRVLAKRAGLEVERLLRTRFGPIRLGVQRP
                     GSLRPLTGREIEALRRAALGREER"
     gene            complement(1929..2597)
                     /gene="scpB"
                     /locus_tag="VFM00_10280"
     CDS             complement(1929..2597)
                     /gene="scpB"
                     /locus_tag="VFM00_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET7498677.1"
                     /translation="MTTTKQILESLLFASDAPVGLSTLVEVLDGPDAATVAEELAQMA
                     RDYEESERGVTLAEIAGGYQLLSKKECAPWIDRMLRSRRKVRLSRAGLETLAIVAYKQ
                     PVTKVEIDSIRGVDSSGSLHTLLERGLVAIKGRSKGVGRPLVYATSTEFLSYIGINDL
                     SDLPELKELGPVLEERERLEMEPDATAEAVAEGSSTASPDAEPAAATESADTEPEPAP
                     EGAA"
     gene            complement(2594..3463)
                     /locus_tag="VFM00_10285"
     CDS             complement(2594..3463)
                     /locus_tag="VFM00_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET7498678.1"
                     /translation="MTEERVDVTAAAPASETGIRVQVPAFEGPLDLLLHLIQQNEIEI
                     TDIPIAKITRQYLETLELMRELDLEVAGEFLVMAATLMRIKARMLLPPPITAEEEEED
                     PREGLVRQLLEYRRFKDASKELERLEGDRRLRFDRGAPAQLEDPEASELLPVSLFRLL
                     DALKGVLSRQAPVPVHTIVAEPIRLEEAISLLANNLRERSRMLFEELLEPFVSRIEKI
                     TAFLGLLEMLKAGNVHVAQEELFGPIWVEWRGEAPPEDAMSDEPAAGEGSAETAEAQS
                     SELPMPDDGEGAR"
     gene            complement(3460..4455)
                     /gene="trpS"
                     /locus_tag="VFM00_10290"
     CDS             complement(3460..4455)
                     /gene="trpS"
                     /locus_tag="VFM00_10290"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET7498679.1"
                     /translation="MSAAPKRPRILSGMRPTGRLHLGNYLGALQNWVALQDSSENFHM
                     VADWHALTTDYEHTDQILPNTLEMVADWLAAGIDPEKSPVFVQSRVKEHAELHLLFSM
                     LVTTSRLERNPTVKDQARDLQLEDHISYGHLGYPVLQAADILLYKANLVPVGEDQVPH
                     VEMTREIARKFNSLYGEVFPVPEPKLTRFARVPGLDGRRMSKSLGNTILLSDTPEEIA
                     AKVKTAYTDPKKIRLTDPGNPDGCVVFAYHRAFNAANAPGIDLACREGRLGCVADKRD
                     LSSVLAEQLAGIRERRKELEAHPERVVEALRDGEARARAVAQETMREVREAMKIG"
     gene            complement(4452..5096)
                     /locus_tag="VFM00_10295"
     CDS             complement(4452..5096)
                     /locus_tag="VFM00_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7498680.1"
                     /translation="MDGVAGMLLLIPPILFALTVHEFSHAYVASRMGDPTARLLGRLT
                     LNPLAHLDPIGTILLLIAGFGWAKPVPVDARYLAHPRRDMMWIALAGPASNVILAALF
                     GTLLRGVDGNAYLSGTIMGAALVDMVRYSVYLNLMLAVFNLIPIFPLDGSRILTGLLP
                     ARAAERYRALEPIGPMLLLGLILIGNLTRVSIIGMLILPVVAVLTRLFTGGLVG"
     gene            complement(5129..6640)
                     /locus_tag="VFM00_10300"
     CDS             complement(5129..6640)
                     /locus_tag="VFM00_10300"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7498681.1"
                     /translation="MPKRLSGVASGCLAAVSGLAAVLILGLPSLAPAGQIPGTAVAQA
                     DPAAAQALPSDTASAPADSSQPVEEWHERVMPVLPERPRVPMYDPGTPVFRKVLKNGV
                     VLMVQEQRTSADVAGMVALRMGTLYEDDQTAGLSQVLMRALTSGTKKLPPADLQLRLL
                     AHKVTLESGASADYGQVGFQTFREEASDAVDLLAQIVLSPSFPDTAVENSRSYFLARA
                     ADDVENPLLATYTYFLGAMYQGSPLARPPHGSVQTLSEARRADVLALYKKLFVGGNMA
                     VSVVGNVDAKKTMAQLEKLFAAAPAGPPPAPPAVAPQAPGADTVIVAERPILARSLVY
                     GYPAPGYADRDYPAFMVIDSYLRSADRSPITFWMPQREQAAAVGVMYPSYPHRSSVAV
                     HLAAKPENYVAARDTVAAVFQQIKTTPFDKGEWGVQVKRVQNGFFWKQNEPVIRARNL
                     SRWEVQGVTTEFPHQFETALLQLKPEDVRDAAARWFTHSAEVSLKPSGRDGQP"
     gene            6831..7331
                     /locus_tag="VFM00_10305"
     CDS             6831..7331
                     /locus_tag="VFM00_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498682.1"
                     /translation="MGRLGMDRVRLRRGNCVLAAGMAISLLAAMPRLAPAASTPSSAD
                     FPSLANAAGVPKAALEQFRGICSDLEAARARLASGRLDESAFADTLLALFSRADTLAQ
                     SLAAGARGNPAWITLQRGVGYLIESLRDNWVGIAAKNGMSFAEADNALKAAVAWRTDV
                     AEASTP"
     gene            complement(7355..8104)
                     /locus_tag="VFM00_10310"
     CDS             complement(7355..8104)
                     /locus_tag="VFM00_10310"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET7498683.1"
                     /translation="MIRRAGRAIVGTRWEAPAKRLYAALTGEKSYLYDYQTIAIMRRV
                     LTPSANAIDAGAFEGGMLRHLCRIAPRGRHLAFEPNPEKFRKLKAAFPGVRVLPYALG
                     ERPGEVPFHRAIEHPALSGLRHRAKYWGDKEPTEEIRVPMETLDRVVPPDDPIAFVKV
                     DVEGGELGLLQGGLGLLRRWRPVIVFESGLGGADYFQTRPEAIYDLLAEATGLKVSLL
                     GAWLDRGAPLSRQGFVDQFEEGRNYYFVAHP"
     gene            complement(8233..8308)
                     /locus_tag="VFM00_10315"
     tRNA            complement(8233..8308)
                     /locus_tag="VFM00_10315"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8273..8275),aa:Ala,seq:ggc)
     gene            8431..9858
                     /locus_tag="VFM00_10320"
     CDS             8431..9858
                     /locus_tag="VFM00_10320"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET7498684.1"
                     /translation="MPPLTGHRYHFSGVGGSGMAPLAMLTVALGADVTGSDRNHDRGV
                     AMHVFEDLRRSGVHLLPQDGSGVTEALTALVHSTAVETSNPDFARAQALGIACVRRGS
                     FLARLAASRRAVAIAGTSGKSTVTAMTAHILVAAGLDPSFLGGGAAVGLAGATPPGGL
                     RVGGSEWFVVETDESDGSVAEFAPAVSVLTNLSRDHKEIEETARNFESLLAGTRERAV
                     IHVSDPELERVRVPETVDVLRVAMPGATWAEPALRAGPIELTPDGVRFEINGVPVRVP
                     FPGILTVENALLACGAAVASGVLLETAAPALASFGGVRRRLERIGGHHGVDVFDDFAH
                     NPVKIRAALRALRPTGSLRVYYQPHGYGPTRFFAEEMTQAFKDELRSGDRLYLAPIYD
                     AGGTADRSIRSEDLVRSMGDRGVPVALAPTREEASRVMTEELRPGDRLLVMGARDDTL
                     PAFARSVYAALVTRDDSPAAQRERA"
     gene            complement(9811..10659)
                     /locus_tag="VFM00_10325"
     CDS             complement(9811..10659)
                     /locus_tag="VFM00_10325"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7498685.1"
                     /translation="MSHRRLLFPFLAVAFLLGPGSAAAGLISLTLVGGATATDGSTTV
                     ADPLAQESLSGTTLTQLGTPLLFDPSGALGLTVRVQNTSDQEIAFPDVLPGVSVLTGV
                     NGITGYSSKLNVAAGGAAGKSGAAGAEGAVSRTVIDLTDSVYAVSSPASGSNGGGGGA
                     LTGVADNWIGLTGASGADLAAYLANVHLAPGQWIDIPDFVRISAFQRMAEGARLGFGF
                     DIPTFSFGGISVTCGAWTGSFSEAGPIAPPPPDPVSLPEPGSGSLWLAGLGAFALCGR
                     RIVARD"
     gene            complement(10855..12405)
                     /gene="pruA"
                     /locus_tag="VFM00_10330"
     CDS             complement(10855..12405)
                     /gene="pruA"
                     /locus_tag="VFM00_10330"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HET7498686.1"
                     /translation="MLPEFQNAALLDFSRPENEKGMQCGLETIRGQLGKTHPLVIGGR
                     RVESSDTFQSVNPADPSQCVGNFAKATPDHVNQAVLAAERAFESWRWVPPAERANVLL
                     RVAGIMRRRRNELSAAMIYEVGKSWPEADGDTAEAIDFCEFYAREMLRWGGDQPVTPY
                     PGEANEFLYIPLGVGAVIPPWNFPLAITTGMTVAAVVTGNTVVLKPSSDAPFIAWKLF
                     EILEEAGVPPGVVNFLPGPGGSMGDALVEHPRVRFVAFTGSKEVGIRIHERAARVPTG
                     QIWLKRMVLEMGGKDFTIVDEGADLDAAAAGVAAGAFGFQGQKCSACSRAIIHRSFYG
                     EFVQRLKARVDAITVGATTSRENYLGPVVSARAEKTILDYIEVGKTEGKTLNGGGKHS
                     GPGYFIKPTVFTDVRPDARIAQEEIFGPVLAVIPAGSFEEEIAIANGTIYGLTGAYYS
                     RDRAKVVEAKRRLHVGNLYINRKCTGALVGVHPFGGFNMSGTDSKAGGRDYLGLFLQG
                     KSITELVG"
     gene            12455..13204
                     /locus_tag="VFM00_10335"
     CDS             12455..13204
                     /locus_tag="VFM00_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498687.1"
                     /translation="MISALATAVIPALLLPWALLPVRMPILFLLLPVVPVAIVYVRAI
                     AARDGGRAVTLALAWAVAFSVSTVAAATARPDAAVHGIWRAGAFRDEMVRWIATGVGQ
                     EGDIRIFLPRVLLEFALVLALSAASMGVAGLCLGAALLGYMNGYVGWVASNADARVGP
                     LAAALIAWPPWSVVRVVAFVCAGTAAALWGHPRFVARAAPRGRWARLLIVAVLLLLLD
                     IFLKWWLAPIWRDWLRSILGASAGIEAGGSA"
     gene            complement(13262..15178)
                     /locus_tag="VFM00_10340"
     CDS             complement(13262..15178)
                     /locus_tag="VFM00_10340"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7498688.1"
                     /translation="MRRFAGFLHTTVAALFVAGGLFTSAPAAAAPPVLSLPGPQTVSE
                     GTDLTFTVSATDPDNQTVFLFAANLPTGATFTDHFNNTGTFDWTPNTDQAGSYVVSFL
                     ADDDFGGTDQGSVSIEVTNANTAPVLFAIGDRTVEQGTTQFVSLTGYDPDGDAVTFSS
                     GPLPAYATLSDYGGGSGNITLAPSLSTPLGTTSITVRLSDGQATVEQSFNVTVTASQV
                     QNRPPVLAAIGNQTVAEGQVRHVAVSASDPDGNNLTWSVTLPSFAVLTPVSSGPGSLA
                     ATLDLSPGYCQSGSYSATISVWDGTALDWETFTITVTNTNRAPAWDQPSYSASLGEGA
                     VTAVAVSASDPDESCTPGAPNLSIKSSDAGAALQASLTDAGDGTGNLTLTAATNGVGT
                     YHVILTAADWADPGLTRDVVVTVTVTHVETGPVARAWSEADPIKLNIGKPRERVYLEP
                     VTGFAVADVDPASLRLTAWTGSGTVEAIAPLVESVDGTHDRDGNGALELRMDFTKDDL
                     RALFANLTTPVAADMTLHATLLNGTAVTAHVSTSVVPERGVIKKLGPNPLNPEAVVTV
                     LTPQDGGLRIRVFDARGRMVRLLMDESNVPAGTHLVRFDGKDDRGKTLASGRYYVQAE
                     TPYTKDVSPVTILK"
     gene            15511..16170
                     /gene="lepB"
                     /locus_tag="VFM00_10345"
     CDS             15511..16170
                     /gene="lepB"
                     /locus_tag="VFM00_10345"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7498689.1"
                     /translation="MAKASPKRQAPKKGETLQDWIKTLVIVLAVVITFRGVVAQAYQI
                     PTGSMERTLLIGDYLYINKMLYGSEIDIGFHGHRYFYHRFPAFRHPEPGDIIVFRYPV
                     NPQQDFIKRCVAVAGQTVEVRDKVLYVDGKRQNEPYAVHDDARVFPREITVRDNFGPI
                     KVPPHCLFMMGDNRDNSLDSRFWGPLPENMVKGKAMFTYFSWDPGEHMVRFNRMFRGI
                     S"
     gene            16217..16516
                     /locus_tag="VFM00_10350"
     CDS             16217..16516
                     /locus_tag="VFM00_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498690.1"
                     /translation="MTQSQVLKRLQRTHWRSLERLTKKAYQAGFEAGLARARGGSRRG
                     RSIRADATVEGLLRRIDRHFGLERYGFEVRVVHPVSGRRVPSRDLLAHHIASNGG"
BASE COUNT         2453 a         5842 c         5742 g         2525 t
ORIGIN      
        1 gatgccgagc tcgcgctcca gctcttcgac gggttttccg ggcacgtagg gcgtgaggct
       61 tcgaatctca ggacggattg gatccatcgc tttgagcgtc atggcgaagc actccttccg
      121 gtcgtgatcc ggagcgcagt ctaccacccc gattcggccg cggggaaact tccgagcacg
      181 cgaaacaggc gcgtccgggc gcgcatttcc gcgagcgccg agcggagcgg atcggcgtcc
      241 tggtgccccg cgacctccag caggaagagg tgctctccgg gcctccagcg atgcgtccgg
      301 gattcgatcc agaggagatt cacgccgtgc gcggccagcg cgcggagcgc ttcgaggagc
      361 gcgcccgggg tgttctccag cgcgagcaga agcgccgtct tgtcgcgccc actcgccgcg
      421 ccggccgagc ggccgagcac caggaaccgc cgcgtgcgac gcggcgcgtc ctcggcctcg
      481 ggctcgacct ccgggaggcc ggccagatcg gccacgatcg ccggcgcgag acagaaagcg
      541 tcggggtcgg ccgccgcgcg ccgtcccgcc tccaggctcc ccgccgcggc aaccacctcc
      601 acgccggggc cgagcgcggc gcgccagcgc tggcagagag cgagcgcggc cggatgggcg
      661 atcaggcgct tcggcgtgcg cgcccggccc agcgcttcgt agccgccccg gtcgacgaac
      721 tcggccgtga tcgccgcctc ggcgcccagg aggagatcgt aggcgtcgaa cggcgggtcc
      781 tcggcgtcgc cctcgagcgc gaagactccg tgctccgcgc gctcggagcc gatcgcttcc
      841 aagaggtgct gcggcgaggc ggtggcggca taggtcgagg aagcgccgaa gcggcgcacg
      901 gcggcctggt gcgcgaagcc tccctcggcg ccgagatagg cgacgcgaaa ggctccctgc
      961 gcggcgcgcg aggcggagac gatctcgcga aagatggcgc gcacgcccga ccggggaaag
     1021 gcgcccttcg cctcgcgctc gatccgctcc agcagatcgc gctcgcgcgc cgcgtccagg
     1081 gtcgcttccc cggaggcctg cttcagcgcc ccgatccgcc gcgccagctc ggcccggtcc
     1141 tcgagcaggc gcagcaggtc gcggtccagc cggtcgatcg cgcggcggat ctcctcgcgc
     1201 tcgccatccg tcatcgctcc tccctcccga gcgccgcccg ccgcaacgcc tcgatctccc
     1261 ttccggtgag cggccggagc gaccccggac gctgcacgcc gaggcggatc ggcccgaacc
     1321 gcgtccggag gagccgctcc acctccagcc ccgcccgctt cgcgagcacg cgcacttcgc
     1381 gcttcttccc ttccgcgaga tcgaagcgga cgatgccacg gccgcgctcg gctccctcga
     1441 ggacgacggc ggcaggacgt accggcaccc cctcgacgac ggctccctcg cgcaggaggc
     1501 ggacgatcgc cggatcgggc acggggcgca cccaaacgcg atagctccgg atgagggcac
     1561 tccgcgggtg cagcagggcc tcggcaagcg cgccgtcggt ggtcaggagg aggagtccct
     1621 ccgattggta atcgaggcga cccacgggca tcatcccgcg cgcctcttcg ccgagccggt
     1681 cgaagatcgt cggctttccc ccctgggagc ggcggctgac gagaacgccg ggcggcttgt
     1741 ggtaggcggc gtagcgttcg gcgtcgggaa ggaggagcac ggcgccgtcg agggcgacct
     1801 ggtcgcgccc cgggaccacc ggagcgccca tgccgtgggg cggaaggccg ttcacggtca
     1861 cgcgcccctc gcggatccac tggtcggcct cgcgccgcga tccgccgccg gcgcgcgcga
     1921 gaaagaggtt aagccgcacc ctcgggcgcg ggctccggtt ccgtgtcggc gctctcggtc
     1981 gccgcggcgg gctccgcatc gggcgaggcc gtcgacgacc cctccgccac ggcttccgcc
     2041 gtcgcgtccg gctccatctc cagccgctcg cgctcctcca ggaccggccc cagctccttc
     2101 agctccggca gatcggagag gtcgttgatc ccgatgtagg agaggaattc cgtcgacgtg
     2161 gcgtagacca gcggccggcc cacccccttc gagcggccct tgatcgcgac caggccgcgc
     2221 tcgagcagcg tgtggagcga gccgctcgag tccacgccgc ggatggaatc gatctccacc
     2281 ttcgtcaccg gctgcttgta ggcgacgatg gcgagcgtct cgagccccgc gcgcgacaga
     2341 cgcaccttgc gccgcgagcg gagcatccgg tcgatccagg gggcgcactc cttcttggac
     2401 aggagctggt agccgcccgc gatctcggcc agcgtcacgc cccgctcgct ctcctcgtag
     2461 tcgcgcgcca tctgggcgag ctcctcggcc acggtggcgg catcgggccc gtcgaggacc
     2521 tcgaccaggg tcgagaggcc caccggcgcg tcgctcgcaa agaggaggga ctctagaatt
     2581 tgcttcgtgg tggtcatcgc gctccctccc cgtcatcggg catcggtaac tcggacgatt
     2641 gggcctcggc cgtctcggcg gagccctcgc cggccgcggg ctcgtcgctc atcgcatcct
     2701 ccggcggcgc ctcgccgcgc cactcgaccc agatcgggcc gaacagctct tcctgggcga
     2761 cgtgcacgtt ccccgccttc agcatctcga gaagtccgag gaacgccgtg atcttctcga
     2821 tccggctcac gaacggctcg agcagctctt cgaacagcat gcgcgagcgc tcgcggagat
     2881 tgttcgcgag gagcgagatc gcctcctcca gccggatcgg ctcggcgacg atcgtgtgga
     2941 ccggcaccgg cgcctggcgc gagaggacgc ccttcagcgc gtcgagcagg cgaaagaggc
     3001 tgaccggcag gagctcgctc gcctcggggt cttcgagctg cgcgggggcg ccgcggtcga
     3061 agcgcaggcg gcgatccccc tccaaccgct ccagctcctt cgaggcatcc ttgaagcgtc
     3121 ggtactcgag gagctggcgg accagccctt cgcgcgggtc ctcttcctct tcctcggcgg
     3181 tgatcggcgg cggcaggagc atgcgcgcct tgatgcgcat gagggtcgcc gccatgacca
     3241 ggaactcccc cgcgacctcc agatccagct cgcgcatcag ctccagcgtc tccagatact
     3301 ggcgcgtgat cttcgcgatc gggatatcgg tgatctcgat ctcgttctgc tggatcagat
     3361 ggagcaggag gtcgagcggg ccctcgaacg cggggacctg aacgcggatc cccgtctccg
     3421 aagccggcgc cgccgccgtc acgtccacgc gctcttccgt catccgatct tcatcgcctc
     3481 gcgcacctcg cgcatcgttt cctgggccac cgcgcgcgct ctcgcttcgc cgtcccgcag
     3541 cgcctcgacc acccgctcgg ggtgggcctc cagctccttc cggcgctcgc ggattccggc
     3601 gagctgctcg gcgaggacgg aggataggtc ccgcttgtcg gcgacgcagc cgagccgccc
     3661 ctcgcggcag gcgaggtcga tcccgggagc gttcgccgcg ttgaaggcgc gatgatacgc
     3721 gaagacgacg catccgtccg ggtttcccgg atcggtcagc cggatcttct tggggtcggt
     3781 gtaggcggtc ttcaccttgg ccgcgatctc ctcgggcgtg tcggagagga ggatcgtgtt
     3841 gccgagcgac ttgctcatcc gccgcccgtc caaccccggc acccgcgcga agcgggtcag
     3901 cttgggctcg ggcaccggga acacctcgcc gtagagggaa ttgaacttcc gcgcgatctc
     3961 ccgcgtcatc tccacgtgag gcacctggtc ctcgcccacc ggaaccaggt tcgccttgta
     4021 gagcaggatg tccgccgcct gcagcaccgg atagcccagg tgcccatagg agatgtggtc
     4081 ttccagctgc aggtcgcgcg cctggtcctt caccgtcggg ttccgctcca ggcggctggt
     4141 cgtcaccagc atcgagaaca agaggtggag ctcggcgtgc tccttcaccc gcgactgcac
     4201 gaacaccggg ctcttctccg gatcgatccc cgcggccagc cagtcggcca ccatctccag
     4261 cgtgttcgga aggatctggt cggtgtgctc gtagtcggtg gtgagcgcgt gccagtcggc
     4321 caccatgtgg aagttctcgc tggaatcctg gagcgccacc cagttctgga gcgcgcccag
     4381 gtagttcccc aggtgcagcc tcccggtcgg gcgcatgccg ctcaggatgc gcgggcgctt
     4441 cggcgcagca ctcatccgac gagccctccc gtgaagagcc gggtcaggac cgcgacgacc
     4501 ggaaggatca gcatgccgat gatcgatacg cgcgtcaggt tgccgatcag gatcagcccg
     4561 agcagcagca tggggccgat cggctcgagc gcgcggtagc gctcggccgc gcgcgcgggc
     4621 aagagcccgg tcaggatgcg cgagccgtcc agcgggaaga tcgggatcaa gttgaagacc
     4681 gccagcatga ggttgagata gacgctgtac cggaccatgt ccacgagggc cgcgcccatg
     4741 atggtcccgg agagatacgc gtttccgtcc accccgcgca agagggttcc gaagagcgcc
     4801 gcgagaatca cgttcgaggc cgggcccgcg agcgcgatcc acatcatgtc gcgccgggga
     4861 tgcgccaggt agcgggcgtc caccgggacc ggtttggccc atccgaagcc ggcgatgagg
     4921 agcaggatgg tgccgatcgg gtcgaggtgc gcgagcgggt tcagcgtgag ccggccgagc
     4981 agcctggccg tgggatcgcc catccgcgag gcgacgtagg cgtggctgaa ctcgtggacg
     5041 gtaagcgcga agagaatggg gggaatgagc agcagcatcc ccgcgacgcc gtccacccgg
     5101 tcctccctgc cccgatgccc gaaggagcct agggctgtcc gtcgcgcccg gagggcttca
     5161 gcgacacttc cgccgagtgc gtgaaccagc gcgcggcggc gtcgcgaacg tcctccggct
     5221 tcagctgcaa gagtgcggtc tcgaactggt ggggaaattc ggtggtgacg ccctggacct
     5281 cccaccggga gaggttccgc gcccggatca ccggctcgtt ctgcttccag aagaacccgt
     5341 tctgaacgcg cttcacctgg acgccccact cgcccttgtc gaagggcgtg gtcttgatct
     5401 gctggaagac ggcggcgacc gtatcgcgcg ccgcgacgta gttctccggc ttggccgcga
     5461 gatggaccgc cacgctcgag cgatgcggat agctcgggta catcacgccg acggcggccg
     5521 cctgctcccg ctgcggcatc cagaaggtga tcggcgagcg gtcggccgag cgcaggtagg
     5581 aatcgatcac catgaaggcg gggtagtcgc ggtcggcgta gccgggggcc gggtagccgt
     5641 agaccagcga gcgcgccagg atcggccgct ccgccacgat cacggtgtcg gcgccgggcg
     5701 cctgcggcgc cacggccggg ggcgccggag gcgggccggc gggtgccgcg gcgaagagct
     5761 tctccagctg ggccatcgtc ttcttggcgt cgacgttccc cacgaccgat acggccatgt
     5821 tcccgccgac gaagagcttc ttgtagagcg cgagcacgtc ggcgcgccgt gcctcggaga
     5881 gcgtctgcac gctgccgtgc gggggccgcg cgagcgggct gccctgatac atcgccccga
     5941 ggaaatacgt gtaggtcgcc agcagcggat tctcgacgtc gtcggcggcc cgcgccagga
     6001 aatagctcct ggaattctcc accgcggtat ccgggaacga cggcgaaagg acgatctgcg
     6061 cgagcagatc caccgcgtcg gaggcctcct cgcggaaggt ctggaatccg acctggccgt
     6121 agtcggcgct cgcgcccgat tccaacgtga ccttgtgcgc gaggaggcgg agctgcagat
     6181 cggccggggg cagcttcttc gttcccgacg tcagcgcgcg catcagcacc tgcgacaggc
     6241 cggccgtctg gtcgtcctcg tacagggtcc ccatgcgcag ggccaccatc cccgccacgt
     6301 cggcgctggt ccgctgctct tggaccatga gcacgacgcc gttcttgagc accttgcgga
     6361 agacgggggt gccagggtcg tacatcggga cgcggggccg ctcgggcagc acgggcatga
     6421 cgcgctcgtg ccactcctcg acgggctggc tggaatcggc gggggccgag gccgtgtcgg
     6481 agggcaaagc ctgggcggcg gccggatccg cctgggcgac ggccgtgccg gggatctgcc
     6541 cggcgggggc caggctcggc aggcccagga tcaggaccgc ggcgaggccg gaaacggccg
     6601 cgaggcagcc tgaggcaacc cccgaaagtc gtttgggcaa aagaagattc gagatcaggg
     6661 acattttccc gaggatagca gacccaccgc gctccgtcaa cgcggcgggc gattcgcacg
     6721 ccgtgtgtac cttgcaaaca ccgttgccgg ggacatttcg cccgttaagt tcgtcccttc
     6781 tggagccgat ttttgaatga ggccgggggg ccttacgaga ggaaggcgaa atgggacgtc
     6841 tcggcatgga ccgcgtgcgt cttcgccgcg gaaattgcgt cctggccgcc ggcatggcga
     6901 tcagcctgct ggccgcgatg ccgcgcctcg ctcccgccgc ctcgacaccc tcttccgcgg
     6961 acttcccgtc cctggccaac gccgccggag ttcccaaggc ggccctcgag caattccgtg
     7021 ggatctgctc cgatctggag gccgcccgcg cgcgtctcgc cagcgggcgc ctggacgagt
     7081 cggcgttcgc cgacacgctg ctcgcgctgt tctcgcgggc cgacaccctc gcccagagcc
     7141 tcgccgcggg cgcccgcggc aatccggcgt ggatcacgct gcagcgggga gtgggctacc
     7201 tgatcgaatc cctgcgggac aactgggtcg ggatcgcggc caagaatggg atgagcttcg
     7261 ccgaggccga caatgcgttg aaagcggccg tggcgtggcg gaccgacgtg gcggaggcat
     7321 cgaccccctg acgtcggttc gcgggcggac gccgtcacgg gtgcgccacg aagtagtagt
     7381 tccttccctc ctcgaactga tccacgaagc cctgccgcga gagcggcgct ccccggtcga
     7441 gccacgcgcc gagcagcgac accttgagcc cggtcgcctc cgcgagcagg tcgtagatcg
     7501 cctcgggccg cgtctggaaa tagtcggcgc cgccgagccc cgattcgaac acgatcaccg
     7561 ggcgccagcg ccggagaaga ccgagcccac cctgcaggag ccccagctcg cccccttcaa
     7621 cgtcgacctt cacgaacgcg atgggatcgt cgggcggaac gacgcgatcc agggtctcca
     7681 tcgggacgcg gatctcctcg gtcggttctt tatcgcccca gtacttcgcg cgatgccgga
     7741 gtccgctcag ggcgggatgc tcgatcgcgc gatggaacgg gacctcgccc ggccgctccc
     7801 ccagcgcata gggcagcacc cggacgccgg ggaacgcggc cttcagcttc cggaatttct
     7861 cgggattggg ctcgaaggcc aggtggcggc cccgcggcgc gatgcggcag aggtgccgca
     7921 gcatgccgcc ctcgaacgcc ccggcgtcga tcgcgttggc gctgggcgtc aggacgcggc
     7981 gcatgatggc gatcgtctgg tagtcgtaga gatagctctt ttcacccgtg agcgcggcat
     8041 agagccgctt ggcgggagct tcccaccggg tgccgacgat cgcgcggcca gctcgtcgga
     8101 tcatgggatc atgggatcat ggggatcggg tcctccgccc ggggtcttcc cgctcgcttg
     8161 gggctactgg gagctacagg aaggggcgaa aggtgccgac agccttggtg ccggaagatt
     8221 tgcgagtaaa gatggtggag ctggcggggt tcgaacccgc tacctcttga ctgccagtca
     8281 agcgctctcc caactgagct acagccccat cctgtcgcag cggaacgaga ttacccttgg
     8341 ctccggagcc tcgtcaacga ccatggactg gaacatccgc cccccggtgc cgcccttgac
     8401 caggcacttc cctgcggcga gtatgcgacc gtgcccccgc tgaccggcca tcgctaccac
     8461 ttcagcggcg tcggcgggag cggcatggcc cctctcgcga tgctcaccgt cgccttgggc
     8521 gccgacgtga ccggctccga ccggaatcac gaccgcggcg tcgccatgca cgtcttcgag
     8581 gacctgcgcc gctccggcgt ccacctgctg ccgcaggacg gatcgggcgt gaccgaagcg
     8641 ctgaccgccc tcgtccattc caccgccgtc gagacgtcca accccgattt cgcgcgggcg
     8701 caggcgctgg ggatcgcctg cgtccggcgc ggatcgttcc tggcccgcct tgccgcctcg
     8761 cggcgcgcgg tggcgatcgc agggacgagc ggcaagtcca ccgtgaccgc gatgacggcg
     8821 cacatcctgg tcgcggcggg cctcgacccc agtttcctcg ggggaggcgc ggccgtcggc
     8881 ctcgcgggcg ccacgcctcc gggcggcctg cgtgtcggcg gctccgagtg gttcgtggtc
     8941 gagaccgacg agagcgacgg cagcgtcgcg gagttcgctc ccgccgtctc ggtgctcacg
     9001 aacctgagcc gcgatcacaa ggagatcgag gagacggccc ggaacttcga gtcgctcctg
     9061 gcgggaacgc gggagcgcgc ggtgatccac gtgagcgatc cggagctgga gcgcgtgcgc
     9121 gtgcccgaga ccgtggacgt cctgcgcgtg gcgatgccgg gggcgacgtg ggccgagccg
     9181 gcgctccgcg cgggccccat cgagctcacc cccgacggcg tccgcttcga gatcaacggc
     9241 gtcccggtgc gcgtcccctt ccccggcatc ctcaccgtcg agaacgcgct gctcgcctgc
     9301 ggcgcggccg tggcttcggg cgtactgctc gagaccgccg ccccggcgct cgccagcttc
     9361 ggcggcgtcc gccgccggct cgagcggatc ggcggccatc acggcgtcga cgttttcgac
     9421 gacttcgcgc acaaccccgt gaagatccgc gccgcgctgc gcgcgctccg ccccacggga
     9481 tcgctccgcg tctactacca gccgcacggc tacggcccca cgcgcttctt cgccgaggag
     9541 atgacccagg ccttcaagga cgagcttcgc tccggcgacc gcctgtacct ggcacccatc
     9601 tacgacgccg gcggcaccgc cgaccgctcg atccgctccg aggatctcgt ccgcagcatg
     9661 ggcgaccgcg gcgttcccgt ggccctggct ccgacgcgag aagaggcatc gcgggtcatg
     9721 accgaggagc ttcggcccgg cgaccggctc ctggtcatgg gcgcccgcga cgacaccctt
     9781 ccggcgttcg cccggtcggt ctacgccgcg ctagtcacgc gcgacgattc gccggccgca
     9841 cagcgcgaac gcgcctagcc ccgccagcca gagcgatcca gaccccggct ccggcagcga
     9901 gaccggatcg ggaggcggag gcgcgatcgg gccggcctcg ctgaaactcc cggtccacgc
     9961 gccgcacgtc acgctgatgc ccccgaagga aaaggtcggg atgtcgaacc cgaaccccag
    10021 ccgcgcgccc tcggccatcc gctggaacgc cgagatgcgc acgaaatcgg gaatgtcgat
    10081 ccattggccc ggcgccaggt gcacgttggc gaggtacgcc gcaaggtcgg cgcccgaggc
    10141 gccggtcagc ccgatccagt tgtcggcgac gcccgtgagc gcgcccccgc cgccgccgtt
    10201 cgatccggac gcgggtgagc tgaccgcgta gaccgagtcg gtgaggtcga tcaccgtccg
    10261 gctcacggct ccctccgccc ccgcggcccc cgacttcccc gcggcgcctc ccgcggcgac
    10321 gttcaacttg gacgaatagc ccgtgatccc gttcaccccg gtgagcacgg agacgccggg
    10381 caggacgtcg gggaacgcga tctcctggtc ggaggtgttc tggacgcgca ccgtcaaccc
    10441 cagcgcgccg gaggggtcga agagcagcgg cgtgccgagc tgcgtgagcg tggtgccgct
    10501 cagcgattcc tgcgcgaggg gatccgcgac cgtggtcgat ccgtccgtcg cggtcgcgcc
    10561 accgaccagc gtcagcgaga tcaacccggc cgcggcggat ccgggcccga gcaggaatgc
    10621 gacagcgaga aacgggaaca gcaggcgacg atgggacacg gctggcctcc gggaatgggt
    10681 ggtgtcggga aggaggccta ttgcgagaga cgtgccgcga ccacgcccag cggcgcgcac
    10741 ccgcgaacgc cgcgtgcggg cgcgacaaaa aggccccgcg ccgttcgaca cggcgcgagg
    10801 ccaaaaccgc gaaacgtcaa cgcacggtga cgcgcgcgcc gacgagcggc gacgtcagcc
    10861 gaccagctcg gtgatcgatt tcccctgcag gaagagtccg aggtaatccc gtccgccggc
    10921 cttggagtcg gtgccgctca tgttgaagcc gccgaaagga tgaacgccga ccaacgcgcc
    10981 ggtgcacttc cgattgatgt acaggttccc gacgtggagg cggcgcttgg cctcgacgac
    11041 cttggcgcga tcgcgcgagt agtaggcgcc ggtcaggccg tagatcgttc cgttggcgat
    11101 ggcgatctcc tcctcgaacg acccggcggg gatgaccgcg agcaccgggc cgaagatctc
    11161 ctcctgggcg atacgggcgt cgggccggac gtcggtgaac acggtgggct tgatgaagta
    11221 cccggggccc gagtgcttgc cccctccgtt cagcgtcttc ccttccgtct ttccgacctc
    11281 gatgtagtcg aggatcgtct tctccgcgcg cgcgctcacg acgggtccga ggtagttctc
    11341 ccggctggtc gtggcgccaa ccgtgatcgc gtccacgcgc gccttcaggc gctgcacgaa
    11401 ctcgccgtag aacgagcggt ggatgatcgc ccgcgagcag gcggagcact tctgcccctg
    11461 gaagccgaac gctcccgcgg cgacgccggc ggcggccgcg tccagatccg ccccctcgtc
    11521 gacgatggtg aagtccttgc cgcccatctc cagcaccatc cgcttcagcc agatctgccc
    11581 cgtgggaacg cgcgccgcgc gctcgtggat ccggatgccg acctctttcg agccggtgaa
    11641 ggcgacgaag cggacgcgcg ggtgctcgac gagggcgtcg cccatcgagc cacccggtcc
    11701 cggcaggaag ttcacgacgc cgggcggcac gccggcctcc tcgaggatct cgaagagctt
    11761 ccaggcgatg aagggcgcgt cgctcgaggg cttgagcacg acggtgttcc ccgtcacgac
    11821 cgcggccacg gtcatcccgg tggtgatcgc gagcgggaag ttccagggcg ggatcaccgc
    11881 ccccacgccg agcgggatgt agaggaactc gttcgcctcg ccgggatagg gcgtgacggg
    11941 ttgatcgcca ccccagcgga gcatctcgcg ggcgtagaac tcgcagaagt cgatcgcctc
    12001 ggccgtgtcg ccgtccgcct ccggccagct ctttcccacc tcgtagatca tcgcggccga
    12061 aagctcgttc cggcggcgcc tcatgatccc cgccacgcgg agcagcacgt tggcgcgctc
    12121 cgccgggggc acccagcgcc aggactcgaa ggcgcgctcg gccgcgagga cggcctggtt
    12181 cacgtggtcc ggcgtggcct tggcgaagtt gccgacgcac tgggagggat cggcgggatt
    12241 gacgctctgg aacgtgtcgc tcgactcgac ccgtcgcccg ccgatcacga gggggtgggt
    12301 cttgccgagc tggccccgga tggtctcgag gccgcactgc atgcccttct cgttctcggg
    12361 acgcgagaaa tcgaggagcg ccgcgttctg gaattcggga agcatgacgg ctcctttacg
    12421 cattcaggtg aatgggggat atactgcggc gcccatgata tccgctctcg ccacggctgt
    12481 catcccggcc ctcctcctgc cctgggcgct cctcccggtc cggatgccga tcctcttcct
    12541 gctgcttccg gtcgttccgg tcgcgatcgt ctacgtccgc gccatcgccg cgcgcgacgg
    12601 aggccgcgcc gtcaccctgg cgctcgcctg ggcggtggcc ttcagcgtct ccaccgtcgc
    12661 cgcggccacc gcgcggccgg acgcggccgt ccacgggatc tggcgcgcgg gcgcgttccg
    12721 cgacgagatg gtgcgctgga tcgcgaccgg tgtggggcag gagggggaca tccggatctt
    12781 ccttccccgc gtgctcctcg agttcgcgct cgtgctcgcg ctctcggccg cgtcgatggg
    12841 cgtggcgggg ctctgcctgg gggcggcgct cctcggctac atgaacggct acgtgggctg
    12901 ggtcgcctcg aatgccgacg cgcgggtggg gccgctcgcg gccgcgctca tcgcgtggcc
    12961 cccctggtcg gtcgtccgcg tcgtggcgtt cgtctgcgcc ggcaccgccg cggcgctctg
    13021 ggggcatcca cggttcgtgg cgcgcgccgc gccgagaggg cgctgggcgc ggctcctgat
    13081 cgtggccgtc ctgctcctcc tcctggacat ctttctcaag tggtggctcg cgccgatatg
    13141 gcgcgactgg ctgcgctcga tcctcggcgc cagcgccgga atcgaagcgg gcgggagcgc
    13201 gtgacgctgg cgcgtccggc tcccgcccag gccgcggggg cgcggcctca ttccgtcggg
    13261 gttacttcaa gatcgtgacc gggctcacgt ccttcgtgta cggcgtctcc gcctggacgt
    13321 agtagcgacc cgacgcgagg gtcttgccgc gatcgtcctt gccgtcgaac cggaccaggt
    13381 gcgttccggc cggcacgttc gactcgtcca tcaggagccg caccatccgg ccgcgggcgt
    13441 cgaacacccg gatgcggagc ccgccgtcct gcggcgtcag caccgtcacg accgcttcgg
    13501 ggttcagcgg gttcgggccc agcttcttga tcacgccgcg ctccggcacg accgacgtgc
    13561 tgacgtgcgc cgtcacggcg gttccgttca gcagcgtcgc atgaagcgtc atgtccgccg
    13621 cgaccggggt ggtcaggttg gcgaagagcg cgcgcaggtc gtccttcgtg aagtccatgc
    13681 ggagctccag ggcgccgttt ccgtcccggt cgtgcgtgcc gtccacgctc tcgacgagcg
    13741 gggcgatcgc ctcgacggtt cccgatcccg tccacgcggt caggcgcagg gaggccgggt
    13801 cgacgtcggc gaccgcgaag ccggtgaccg gctccaggta gacgcgctca cggggcttgc
    13861 cgatgttgag cttgatcggg tccgcctcgg accaggcgcg cgccaccggc ccggtctcga
    13921 catgcgtcac cgtgaccgtc acgacgacgt cgcgcgtcag gcccggatcc gcccagtcgg
    13981 cggcggtcag gatgacgtga tacgtcccca cgccgttcgt cgcggcggtg agcgtcaggt
    14041 tgcccgtgcc gtcaccggcg tcggtcaacg aggcctgcag ggccgcgccg gcgtcggagc
    14101 tcttgatgct caagttcgga gccccgggcg tgcacgattc atccgggtcg ctggcgctca
    14161 cggcgacggc cgtgacggcc ccttcgccga gcgaagccga gtacgacggc tggtcccacg
    14221 ccggagctcg gttcgtgttc gtgaccgtga tcgtgaacgt ctcccagtcc agggccgttc
    14281 cgtcccagac ggagatcgtc gccgagtagc tgcccgactg gcagtagccc ggagacaggt
    14341 cgagcgtcgc cgccagggag cccggaccgc tgctcaccgg cgtcagcacc gcgaagctcg
    14401 gaagcgtgac gctccacgtc aggttgttgc cgtcgggatc gctggccgag accgcgacgt
    14461 ggcgcacctg cccctcggcg accgtctggt ttccaatcgc cgccaggacc ggcggacggt
    14521 tctgcacctg gctcgccgtg accgtcacgt tgaacgactg ctccacggtg gcctgcccgt
    14581 cggagaggcg gaccgtgatc gacgtcgttc cgagcggcgt ggacaaggac ggcgccagcg
    14641 tgatgttgcc cgacccgccg ccgtagtcgc tcagcgtcgc gtacgccggc agagggccgc
    14701 tcgagaaggt gaccgcgtcg ccgtccgggt catagccggt cagcgacacg aactgcgtgg
    14761 ttccctgctc caccgtgcgg tcgccgatgg cgaacaggac cggcgccgtg ttggcgttcg
    14821 tcacctcgat cgagacggag ccctggtcgg tgcctccgaa atcgtcgtcg gcgaggaacg
    14881 agacgacgta gctccccgcc tggtcggtgt tcggcgtcca gtcgaacgtg ccggtgttgt
    14941 tgaagtggtc ggtgaacgtc gcccccgtcg gcaggttcgc cgcgaagagg aagaccgtct
    15001 ggttgtcggg atcagtggcg ctcacggtga acgtgagatc cgtcccctcc gacacggtct
    15061 gcggaccggg cagggagaga accggaggcg ccgcggcggc cggcgcgctc gtgaagagcc
    15121 cgcccgccac gaagagcgcg gcgaccgtcg tgtgcaggaa acctgcgaag cggcgcatac
    15181 gaacccccag tcattcggac gaaacggaac ttcgcgcccc atccgtgggg tccgctcgac
    15241 tcccgtgaag taatggttcg gtccgtgagg atgcatagcg tgggccattg ccggaaaatg
    15301 gcacagggac gctgtaagac cgatacttac ctcggagttg gcggtagcgg acgcgttccc
    15361 gggagttgcg ttccgcgtgg ctcgatccgg ccggcacagc cacatccggt cggatccggc
    15421 caccattcac gtggaaccgc acgaggcctc ccgccatact aaagggagac aacgtcgtcc
    15481 cgttttccat cccatccagg aggcagcaac gtggcgaaag cgagccccaa gcgtcaggcc
    15541 ccgaagaaag gcgaaacgct tcaggactgg atcaagaccc tggtgatcgt cctggccgtg
    15601 gtcatcacct tccgcggcgt ggtggcgcag gcctaccaga tcccgaccgg ctcgatggag
    15661 cggacgctcc tgatcggcga ctacctctac atcaacaaga tgctctacgg gtcggagatc
    15721 gacatcggct tccacgggca tcgctacttc tatcaccgct tccccgcgtt ccggcacccg
    15781 gagcccggcg acatcatcgt gttccggtat ccggtcaacc cgcagcagga cttcatcaag
    15841 cgctgcgtgg cggtcgcggg acagacggtg gaggtccgcg acaaggtcct ctacgtggac
    15901 gggaagcggc agaacgagcc gtacgccgtt cacgacgacg cgcgggtctt cccgcgcgag
    15961 atcacggtgc gcgacaactt cggaccgatc aaggtcccgc cccattgtct cttcatgatg
    16021 ggcgacaacc gcgacaacag cctcgacagc cgcttctggg gtcccctgcc ggagaacatg
    16081 gtcaagggaa aggcgatgtt cacctacttc tcgtgggatc ccggcgagca catggtgcgc
    16141 ttcaatcgga tgttccgggg gatcagctag agggaggcgg atccccgccg gacgccggaa
    16201 caggaggaat cgtctcatga cccaatccca ggtcttgaag aggctccagc gaacccactg
    16261 gaggagcctc gagcgactca ccaagaaggc gtaccaggcc ggcttcgagg ccggccttgc
    16321 ccgcgcgcgc ggcggatcgc gccgcggacg cagcatccgc gccgacgcga cggtcgaagg
    16381 actcctgcgt cgaatcgacc gccacttcgg gctggagcgc tacgggttcg aggtgcgcgt
    16441 cgtgcacccc gtgtcgggcc gccgggtgcc ctcccgggac ctcctggccc atcacatcgc
    16501 ctcgaacgga ggctgagttt tcagcggcgc cgcccgtgcg ccgcagctga accgcgctcc
    16561 cg
//
LOCUS       DASSLZ010000273         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_857623, whole genome shotgun sequence.
ACCESSION   DASSLZ010000273 DASSLZ010000000
VERSION     DASSLZ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857623"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="VFM00_10355"
     CDS             complement(<1..1288)
                     /locus_tag="VFM00_10355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET7498691.1"
                     /translation="MTIRAFLPLGNGGSAPLADVPCLQSDPFREAVVTTPAARLVSLF
                     GFPDEDAVRVVAILERPEDRNLLVTSMRAGEAFPALTPDMPQAHLFEREIAETWGLVP
                     EGHPWLKPVRFVRPRRAPAGAATTALEAPRPGVMGYFAVAGEEVHEVAVGPVHAGVIE
                     PGHFRFQCHGERVIHLEISLGYQYRGIERAMEGGPDARTVHRVDAVAGDMSAGHASAY
                     AQIVEALTDTPVPQRGAALRAIALELERMANHAGDLGALSGDIGYLPAAAFCGRIRGD
                     LLNLTLAACGNRMGVGWIRPGGVAADLSDEGAHAMAAAVDRAVAELGRSFLLILKNST
                     ARSRLEETGVLPRETAVELGMVGPAARASGVAVDVRKDHPLGPYALAPLPVALRESGD
                     VFARAAVRSLECAHSAEYLAKLLRSLPAGPARSGLPA"
     gene            complement(1285..2775)
                     /locus_tag="VFM00_10360"
     CDS             complement(1285..2775)
                     /locus_tag="VFM00_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006008034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET7498692.1"
                     /translation="MLSALVLIPAAAGLLAFFIRPAPWRRALLLLTAGAHAGLTASLW
                     VRAPGPGWGGVLALDAIGQLFLTITSGLFLVAAVYAVGYLGREFPGAREDFVEGFRFT
                     NAPEAVFTGCLLFFLATMTLVVVSDHLGLLWVAIEATTLASAPLIYFHRHHRSLEATW
                     KYLLICSVGIGLALLGNFFLVVAATAPGGASLPLSAKLLTEQAAALQTPWLKGAFLFL
                     LIGYGTKMGLAPLHTWLPDAHSEAPSVVSALLSGALLNCAFVGILRVLQICGAAGLGT
                     YTGGMLIVFGLLSIALAAAFLVAQADYKRLLAYSSVEHMGILALGVGVGGAATFGAML
                     HAVNHSVVKAMLFLLAGNILAAYRTKTARDVRGLFRRVPVTGALWVAGLFAIAGSPPF
                     GTFASELLILRGALEQGRFAIAALYLALLTVAFVGMAATMTRMAQGEGPSPAMPGAAG
                     ESRLAVLPPLALLAASLILGVYLPAGLSAALRSAAALVGAGVPAIR"
     gene            complement(2778..>3392)
                     /locus_tag="VFM00_10365"
     CDS             complement(2778..>3392)
                     /locus_tag="VFM00_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HET7498693.1"
                     /translation="LLVFTNLRLLGLSRLNASIRTVAVQGVLLGVTAILANAGHLTPP
                     FVAIGAAGILLKGVVFPWLLFRALRGAKIRREVEPFVGYVPSVLAGVVAFLIALWVAR
                     RLPLPAAAASDLLVPTSLSILFVGLFLVVSRKKAVSQALGYLVFENGIYAFGVGIAYQ
                     APVLVEMGILLDVFVAVFVMGITVFHISREFDSIDTTRLAALKE"
BASE COUNT          592 a         1186 c         1246 g          368 t
ORIGIN      
        1 cggcaggaag gccgctccgc gccggaccgg caggcagcga gcgcagcagc ttcgccaggt
       61 actcggccga gtgcgcgcac tccagcgagc gcacggcggc gcgcgcgaac acgtcccccg
      121 actcacggag ggcgacggga aggggtgcta acgcatacgg gccgagcgga tggtccttcc
      181 ggacgtccac cgcgacgccc gacgcgcgcg cggcgggtcc caccatgccg agctccactg
      241 cggtctcgcg gggcagcacg ccggtctcct ccaggcgcga ccgggccgtg gagttcttga
      301 ggatcaagag gaaggaacgc cccagctcgg cgaccgcccg atccacggcg gcagccatcg
      361 cgtgcgcgcc ctcgtcggaa agatcggcgg ccacgcctcc gggccggatc catcccacgc
      421 ccatccggtt gccgcacgcg gccagcgtga ggttcaggag atccccgcgg atccgcccgc
      481 agaacgcggc ggccggcagg tagccgatgt cgccggagag cgctccgagg tctccggcgt
      541 gattggccat ccgctccagc tcgagcgcga ttgcgcggag cgccgctccc cgctggggga
      601 cgggcgtgtc ggtcagcgcc tccacgatct gcgcgtaggc ggaggcgtgt cccgcggaca
      661 tgtcccccgc gacggcgtcc acgcgatgga cggtgcgggc gtcgggacct ccctccatcg
      721 cgcgctcgat gccccggtac tggtagccca gggagatctc cagatggatc acgcgctcgc
      781 catggcactg gaagcggaag tggccgggct cgatcacgcc ggcatggacc ggaccgaccg
      841 ccacctcgtg aacctcctcg cccgccacgg cgaaataccc catgacgccg ggccgcggcg
      901 cttccagggc cgtcgtggcc gcgccggcgg gtgcgcggcg gggccggacg aaccgtaccg
      961 gcttgagcca cgggtgccct tccggcacca ggccccacgt ctccgcgatc tcccgttcga
     1021 agaggtgcgc ctggggcatg tccggggtga gcgccgggaa ggcctcgccg gcgcgcatcg
     1081 aggtcacgag caggttccga tcctcggggc gctcgaggat cgccacgacc cggaccgcgt
     1141 cctcgtccgg aaatccgaag agggagacca gccgcgccgc gggcgtcgtg acgaccgctt
     1201 cgcgaaacgg gtccgactga aggcagggca cgtcggcgag cggcgccgat ccgccatttc
     1261 cgagaggaag aaacgcgcgg atggtcatcg gatggcgggg acgccggcgc caacgagcgc
     1321 cgccgccgag cggagcgcag ccgagagccc cgccggaagg tagacgccga ggatgagcga
     1381 cgccgccagg agggcgagcg gcggcagcac ggcgagacgg ctctccccgg cggccccggg
     1441 catcgccggc gacggcccct cgccctgggc catgcgggtc atcgtggccg ccatcccgac
     1501 gaacgccacc gtcaggagcg ccaggtagag cgcggcgatc gcgaagcgcc cctgttccag
     1561 ggcgccgcga aggatcagga gctcgctcgc gaaggtcccg aagggcggcg agcccgcgat
     1621 cgcgaagagt cccgccaccc agagcgcgcc cgtcaccgga acgcggcgga agagcccgcg
     1681 gacgtcgcgg gcggtcttgg tgcgataggc cgcgaggatg ttccccgcga ggaggaagag
     1741 catcgccttg acgacggagt ggttcaccgc gtgcagcatc gcgccgaagg tggcggcccc
     1801 gcccacgccc acgccgagcg cgaggatgcc catgtgttcc acgctggagt aggcgaggag
     1861 ccgcttgtag tcggcctgcg ccacgagaaa cgccgccgcg agcgcgatgg agaggaggcc
     1921 gaacacgatg agcatccccc ccgtgtaggt gccgagcccg gccgcgccgc agatctgcag
     1981 cacgcgcagg atccccacga aggcgcagtt caagagcgcg cccgagagga gcgccgaaac
     2041 caccgagggc gcttcgctgt gcgcgtcagg gagccaggtg tggagcggtg ccaagcccat
     2101 cttcgtgccg tagccgatca gaaggaacag gaacgcgccc ttgagccagg gggtctgcag
     2161 cgcggccgcc tgctcggtca ggagcttggc actcagaggc aggctggcgc cgcccggcgc
     2221 ggtcgcggcc accaccagaa agaagttccc gagcaaggcg agaccgatcc ccaccgagca
     2281 gatgaggaga tacttccagg tcgcctcgag cgagcggtga tggcggtgga agtagatgag
     2341 cggcgcgctg gccagcgtgg tcgcctcgat ggcgacccag aggagcccga gatggtcgct
     2401 cacgaccacg agcgtcatcg tggcgaggaa gaagagcagg cacccggtga acaccgcctc
     2461 cggggcgttc gtgaagcgga acccctcgac gaaatcctcc cgcgcccccg ggaactcgcg
     2521 gccgaggtat ccgacggcgt agaccgccgc gaccaggaag agcccgctcg tgatcgtcag
     2581 gaagagctgg ccgatcgcgt ccagggcgag cacgccaccc cagccgggac cgggtgcccg
     2641 gacccagagc gaagcggtga gccccgcgtg cgcgcccgcc gtgaggagca gaagcgcgcg
     2701 gcgccacggc gccggccgga tgaagaaggc gaggaggccg gccgcggccg ggatcaggac
     2761 cagcgcggag agcatcgcta ctccttgagc gcggcgagcc gggtcgtgtc gatgctgtcg
     2821 aactcgcggc tgatgtggaa caccgtgatg cccatgacga acaccgccac gaacacgtcg
     2881 agcaggatcc ccatctcgac cagcacgggc gcctggtagg cgatccccac gccgaaggcg
     2941 tagatcccgt tctcgaagac caggtagccg agcgcctggc tcaccgcctt cttccggctc
     3001 accacgagga agagccccac gaagaggatc gagagcgacg tggggacgag gagatcggag
     3061 gccgccgcgg ccggaagcgg caggcggcgc gccacccaca gggcgatcag gaaggccacg
     3121 actccggcga gcaccgacgg gacgtagccg acgaagggtt ccacctcgcg gcggatcttc
     3181 gccccgcgaa gcgcgcggaa gaggagccag gggaagacga cccccttgag caggatgccg
     3241 gcggcgccga tcgcgacgaa cgggggcgtc aggtggcccg cgttcgccag gatggcggtc
     3301 acgccgagca gcaccccctg caccgccacc gtccggatgc tcgcgttcag gcggctcagc
     3361 ccgagcaggc ggagattcgt gaagaccagg ag
//
LOCUS       DASSLZ010000274        12453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_355995, whole genome shotgun sequence.
ACCESSION   DASSLZ010000274 DASSLZ010000000
VERSION     DASSLZ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12453
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355995"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /gene="carB"
                     /locus_tag="VFM00_10370"
     CDS             <1..1538
                     /gene="carB"
                     /locus_tag="VFM00_10370"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET7498694.1"
                     /translation="CCCHGSFAFREEGFETIMVNCNPETVSTDYDTSDRLYFEPLTFE
                     DVMNVIEREKPEGVVIQFGGQTPLRLALPLHRAGVPILGTSPDAIDLAEDRRRFSTLL
                     DELGIPQPESGTATSLEGAKEVAARVGYPVLVRPSYVLGGRAMAIVYAEAHLEQYVRE
                     AVRTSPEHPILIDRFLEHAQELDVDAVADGERVVIGGILQHIEEAGIHSGDSCAVLPT
                     FDVPERHLETIRDATRRLGLRLGVRGLMNVQFAIQRDRVYVLEVNPRASRTVPFISKA
                     TGVPLAKVAARVMAGRSLAEQGITTDLTVDRFFIKAPVFPFLKFPGADILLGPEMKST
                     GEVMGISENFGIAFAKAMSAAGSEIPTSGTAFVSVNDHDKEPVLPIAAALHEMGFALI
                     ATRGTAAFLESRGVPCAMIYKVNEGRPHVVDRIKSRGIDLIVNTPLGEASFYDDGAIR
                     KNAILYGVLCLTTITAAAATVEAIRALRERGASEVVSLQEIHAPAVEKTQEDTSTAVV
                     SQR"
     gene            1713..2444
                     /locus_tag="VFM00_10375"
     CDS             1713..2444
                     /locus_tag="VFM00_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498695.1"
                     /translation="MILVVPVDPQFLPETMLLIAAVFSGVAAIRFLVLGHRANAARAA
                     AGALGCIVFAFVLAKTMHASPETLGLVPAAKSDTAAPTATTTLVLGGVLVRVASADQY
                     VLSVEGEPFLTLDLQRSKLRVTCTVQSEQGAAAKIVQNSVPVRAGFLDTRRDPHTLLV
                     LAGGKNVLRVHYATPARVEIEGDFFERKREDDTQVSIHLISFRKGIEWAGGRIAEGTT
                     VDLTAQGPGRVDFGSTGAVRVVPGK"
     gene            complement(2452..3117)
                     /locus_tag="VFM00_10380"
     CDS             complement(2452..3117)
                     /locus_tag="VFM00_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET7498696.1"
                     /translation="MPKPKRATSPDYIDHAVRHLRDADPTLAKLMDRVGDCGFHATRS
                     TKSPFLALAESIAYQQLNGKAAATIWGRFDALFPGTGPTPEGVLAASDEAMRGAGLSR
                     AKMLAIRDLAAKTLDGTVPTSARLRGLDDEEIVERLTSIRGIGRWTAEMLLMFRLGRP
                     DVLPATDYGVRKGFSIAYRKRSLPTPAKLLAHGERWRPYRSVASWYLWRAVDLGGEKK
                     GRS"
     gene            3174..4562
                     /locus_tag="VFM00_10385"
     CDS             3174..4562
                     /locus_tag="VFM00_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7498697.1"
                     /translation="MARRLRTDLPFKRIAVVLSGGGALAAYETGVLRTLERIGLRPAI
                     VSGASAGALNAVAWVAHDFKTAALERVWRHVDAAEIGMRWTTVALRAAGGLLLVLGLM
                     QALVSVTGSSDLSFLALFHHGAPVGLWPTSSFLDIMAWLLVAAGGYALLRSSREAEKF
                     LARFGDPDSERRVRLWGAIILLLWSIAHFTTWLLAWPWPHRFSATLLAVTAGVWFVTR
                     SQRAGAIARRVLFLLMPETDGRGIWGSAARRRLLRRLIGVRRLERLWPGETHLILSAL
                     ALDNGRIAHFVNWPATKARFHARVETALGEVIPLVGPEEVVEAAIASSAIPVLFEPAR
                     IGGRDFVDAVAFSMHPLRAAIYDDADAAVVIVVAPSGAPPAPRPPRNPFEVWGRYLDL
                     ANWRDLQQELVSLPGDWHSKEAPHALCLVEPEAALPGGTLAYSPKIAARLIRRGELDA
                     LRALDRAGWLET"
     gene            4563..5309
                     /locus_tag="VFM00_10390"
     CDS             4563..5309
                     /locus_tag="VFM00_10390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HET7498698.1"
                     /translation="MPLLTREWPRLPEARRGYVDAIFRRIAPRYDFLTRVLSFGQDRR
                     WKEAVIARLPAPRENGVILDLATGTGELPLLARKRGRPERVVAVDRSRSMLERGRVKV
                     AAAGADRIHFVAGDLNTVPVAEKAFDAVLIGYGLRYPQDLRRVLAEILRSLRPGGVFV
                     SLDFGVPRNGFLRRVMFGYLLGMGTVWGALLHGKPGTYWHIVESLRAYPGQARLAELM
                     EEVGYERVRVEERLFGSSAIVTGVRGPVTA"
     gene            complement(5299..5892)
                     /locus_tag="VFM00_10395"
     CDS             complement(5299..5892)
                     /locus_tag="VFM00_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498699.1"
                     /translation="MAGSRLPDPPPAKGPRPDAIFVFAGGQARKECAAGLWQVLMHAS
                     DAPEMALVVSVARFEWRRYALLGLPGVDALQEAVDVVPPEQRHFFVILERNNVEVRHV
                     TPRRWGTRNEARALAGLVAERGWRNLLVITSKFHLARVRWVVRRALAGRGVAVVYATP
                     SRGADPHGPGRWFRTRRGIRLVASEQVKRLVYRFLKP"
     gene            complement(5895..7148)
                     /locus_tag="VFM00_10400"
     CDS             complement(5895..7148)
                     /locus_tag="VFM00_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HET7498700.1"
                     /translation="MSPEAEAALHVAIAGLGGAAIGIERQWSGHATGPRARFGGIRTF
                     TLLGLLAGAAGLCWALQAPELGAVLAAGGAAVVAGGYWKLHREDVEATTEVAALVVIA
                     AGVLAGAGRLVLASGMIAVTALLLVEKSRLHHAVSHLDSREVRAAAQFLVMAVVILPL
                     LPQGPYGPLGGIRPRQLWILVLFFSGLSYLGFIARRVIGEKHGYLVAGLLGGLISSTS
                     TTLTFSRQSREHPEAGPPLAFGILAACALLYVRVVVATAVLNTDLMTQTLPYLAAPFA
                     VTVLVTLIGIRRARDNHASMPEPRNPLQLKSALQMAAIFQVVLFAVHAVRTLWGGAGL
                     LASGAVLGLTDVDALTISMARTAEDPSMLLIAAQAIAVGVLSNTVFKMGIALAVGRGA
                     VRTIAAAGLLLGAVASAASLYLMLR"
     gene            complement(7293..8462)
                     /locus_tag="VFM00_10405"
     CDS             complement(7293..8462)
                     /locus_tag="VFM00_10405"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HET7498701.1"
                     /translation="MLFPLGVLYGVVGALLWPLHAAGLIPYPAVTHWTLMIQGFQHAF
                     ILGFLLTSMPAWMHSLPSRPWELATATVLMLGVGTAALLGSILAVQLFYVATLALVVV
                     MALRRVSKSTSPPPEEFLLVAFGLLLGFAGAIMAGGAALGAWAEPSPRFGLRLIWMGM
                     ILSIVLGVGGLLVPTFTSMRDPLVIPGIAKPHVRKPRRILYALIALAMLGSLLLEASG
                     RLQAGALVRAVTGSLVLLLVWKLFRLPGRHDRVSYFLWGSGWMIFAGLWLMALAPHLP
                     LLGAHTLFIGGFGTLSAGIATRVVIRHGSYPIVQEGFVLRPAMALLLATAVVTRLLAE
                     LLPSARMAMLSVSGSAWILAWLVWIAGALPRVLRRAPAEKVAFQRSPSSNPPSPF"
     gene            complement(8555..9436)
                     /locus_tag="VFM00_10410"
     CDS             complement(8555..9436)
                     /locus_tag="VFM00_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7498702.1"
                     /translation="MNAAPIAAVAPVATPSVPGRPRRIVVIAREEYRRALETRWLFAF
                     TALFAALVLGVSFFGLVQSGEVGFQGFSRVTLSLLNLVLFTVPLTGLVLGVGSVSGSA
                     ETLPLLLAQPVGRGEVLAGKYLGLTAALGAAQAIGFGAGGLIVALQAGPEQIRGFAAL
                     TAVSLLLGSLSLAAALALASILADRVKALAAAVFLWLLLVIAYDLAVLGATALLRGVP
                     LQSVLFPALLLNPVDLARVLVTLAVGSGALFGPTSAVLVKLLGGGGGALLGLAVLLVE
                     TAVPLAVAARVFGRRDW"
     gene            complement(9433..9846)
                     /locus_tag="VFM00_10415"
     CDS             complement(9433..9846)
                     /locus_tag="VFM00_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7498703.1"
                     /translation="MKRYGLLALVVLAGAALILAAARPRSAAVAPRAVPANARAERTL
                     SLVVQGGAVLPARIEVPKGAAVLVRVENRDPSAHQFSLLGYESAVSPAALSPGGAATV
                     RFTADRPGSDFAWLLDGKPAGVFLVQGSHLVEGHR"
     gene            complement(9843..10775)
                     /locus_tag="VFM00_10420"
     CDS             complement(9843..10775)
                     /locus_tag="VFM00_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7498704.1"
                     /translation="MIQLHDVTKRYGAVTAVDGLSFSVEPGETFALIGPNGAGKTTTL
                     KLILGLARPTSGSIAVGLAGLPPHAAETRRGMGYVPQRVDLPAGRTAAEVLGFFAALR
                     GLDGYAVSRALDRVGMTALAGRRASELSGGYAQRLLLAQALLGDPSLLVLDEPTASLD
                     PEATWEFRSLVEGLRQDGKTILLCSHLLAEVERVADRVLILAGGRRVALESLADLRAR
                     QASSARLEIEARNTEAAGAALARRGIAFTPWGERRVAIEGANGRGVAALEALREAGVE
                     IRTFELTRPTLEEIFLAAVRKERDARNAPPESNP"
     gene            complement(10772..12247)
                     /gene="nosD"
                     /locus_tag="VFM00_10425"
     CDS             complement(10772..12247)
                     /gene="nosD"
                     /locus_tag="VFM00_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HET7498705.1"
                     /translation="MNPRASERLASALQIALLVALLAAVALTLLLVMGAASPHAGAST
                     IRVAPGQAAARLLGAGDGDTLLLEPGVHPGPLVVTRRVALIGVPGAVVDGGGRGSIIT
                     VLAPGVRIERLALRRSGDRFEDLDAGVRLSSAPGARLAGLRIEDVRYGVAAERSDDLD
                     VEDCSLTGRVVPSVDAAPEMDVAAGNGVHVWYSRGARIRRTEIRRFMDGIYLSFADSI
                     RIEGCGSSGNGRYGLHTMYCQDNRIVGNRFTGNIAGCAIMFSNHLELARNDFMHNRGP
                     RTYGILLRDCSDGFFHENRLVDNTIAVFMDGSNRNRIHGNLVQDDGWGVILFSSCDGN
                     EFAGNDFVNIDYPVALDMRRTDNRFDDGARGNYWSASAPYDLNADGIGDVPYAPVSAF
                     AFLSKQYPDLAIFGESPAAGALSLAERTIPALRPSEAVDHYPLVRPAGAGVGSRSAAG
                     VSPAASSSAGPVHREVHPAAAGFLLLAGAGVIGIARRKAAR"
     gene            complement(12244..>12453)
                     /locus_tag="VFM00_10430"
     CDS             complement(12244..>12453)
                     /locus_tag="VFM00_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498706.1"
                     /translation="LNPHAAITMKAFMPPVIGYSKMANFHVLSLPGPGTLVLGIAWLL
                     GPLALWMERRADRRAHAPAAAAVAA"
BASE COUNT         1969 a         4229 c         4557 g         1698 t
ORIGIN      
        1 actgctgctg ccacggctcg ttcgccttcc gcgaggaagg gttcgagacg atcatggtga
       61 actgcaaccc cgagaccgtc tccaccgact acgacacgtc ggaccgcctc tacttcgagc
      121 cgctcacctt cgaggacgtg atgaacgtga tcgagcgcga gaagcccgaa ggggtggtga
      181 tccagttcgg cgggcagacg ccgctccggc tggcgctgcc gctccatcgc gcgggcgtgc
      241 cgatccttgg caccagcccc gacgcgatcg acctggccga ggatcggcgc cgcttcagca
      301 cccttctcga cgagctgggc attccgcagc ccgagagcgg cacggccacc tcgctggaag
      361 gcgcgaagga ggtcgccgcg cgcgtcggct atccggtgct ggtgcgcccc tcgtacgtcc
      421 tgggcggccg cgccatggcg atcgtctacg ccgaggcgca tctcgagcag tacgtccgcg
      481 aggcggtgcg cacctcgccc gagcacccga tcctgatcga ccgcttcctc gagcacgccc
      541 aggagctgga cgtggacgcg gtggcggacg gggagcgggt cgtgatcggc gggattctgc
      601 agcacatcga ggaggcgggc atccacagcg gcgactcgtg cgccgtgctg ccgacgttcg
      661 acgtgccgga gcggcatctc gagacgatcc gcgacgccac gcggcggctg gggctccggc
      721 tgggcgtgcg cgggctgatg aacgtccagt tcgcgatcca gagggaccgc gtctacgtcc
      781 tggaggtgaa tccccgcgcc tcgcggacgg tgccgttcat cagcaaggcg accggcgtgc
      841 cgctggccaa ggtcgcggcg cgggtcatgg cgggacggtc cctggcggag caggggatca
      901 ccaccgatct cacggtggat cggttcttca tcaaggcccc cgtgtttccc ttcttgaagt
      961 tcccgggcgc cgacatcctg ctcggccccg agatgaagtc gaccggggag gtcatgggga
     1021 tctcggagaa cttcggcatc gccttcgcca aggcgatgtc ggccgcggga tcggagatcc
     1081 cgacatccgg gaccgcgttc gtgagcgtga acgaccacga caaggagccg gtgctcccga
     1141 tcgccgccgc gctgcacgag atgggtttcg ccctgatcgc gacgcgcgga accgcggcct
     1201 tcttggagag tcgcggcgtt ccctgcgcga tgatctacaa ggtgaacgaa ggccgcccgc
     1261 acgtggtgga ccggatcaag agccgcggaa tcgatttgat cgtgaacacg ccgctcggcg
     1321 aggcgtcgtt ctacgacgac ggcgccatcc ggaagaacgc catcctctac ggcgtcctat
     1381 gcctcacgac gatcaccgca gccgcggcga cggtggaagc gatccgcgcc ctgcgtgagc
     1441 gcggcgcgtc ggaggtggtc agtctgcagg agatccacgc gcctgcagtc gagaagacgc
     1501 aggaagatac gagcaccgcg gtggtgagtc agcgctagtc gacaacatct gctgcaccgg
     1561 acccccgttt cgtatacacg cgccggggaa gaccgacccc aaggcgcgag gcgcttactc
     1621 cgctgttgcg tgctgtgaga ccgagtgaca cacgcaacat actccagagt atgtcagcag
     1681 cctgaccggc ggatggagcc aagcagggcg ccatgatcct cgtcgttccc gtcgacccgc
     1741 agttccttcc ggaaacgatg ctcctgatcg cggccgtatt ttccggcgtg gcggcgattc
     1801 gctttctcgt gctcggacac cgcgcgaacg cggcaagggc agccgccggg gcgctgggct
     1861 gcatcgtctt cgccttcgtg ctcgccaaga cgatgcatgc ttcaccggaa acgctggggc
     1921 tggtaccggc tgcgaagagc gacacggcgg cccccaccgc aacgacgacc ctcgtgctgg
     1981 gaggcgttct ggtgcgcgtc gcgtcggccg accagtacgt cctgtccgtc gagggggagc
     2041 ccttcctgac gctcgatctc cagcgatcga agctgcgcgt gacgtgcacg gtccaatccg
     2101 agcagggagc cgcagcgaag atcgtccaga attccgttcc cgtgcgagcc ggtttcctgg
     2161 acacccgccg ggatccccac acgctgctcg tcctggccgg cggcaagaac gtgttacggg
     2221 ttcactacgc gacccccgca cgcgtggaga tcgaaggcga cttcttcgag cggaagcgcg
     2281 aggacgacac ccaggtctcc attcacctga tctcgttccg gaagggaatc gaatgggcgg
     2341 gaggccggat cgcggaaggg actaccgtgg atttgaccgc tcagggccct ggtcgggtcg
     2401 atttcggttc caccggcgcc gttcgcgtcg tcccggggaa gtgaaccggc gtcagctcct
     2461 accctttttc tccccgccca gatccaccgc tcgccacaga taccagctcg ccaccgaacg
     2521 atagggcctc catcgttcgc cgtgcgcgag cagcttcgcg ggcgtgggga gcgagcgctt
     2581 ccgataggcg atggagaatc ctttccgcac gccgtagtcg gtggcgggga ggacgtcggg
     2641 ccggccgagg cggaacatca ggagcatctc ggcggtccac cgcccgatcc cgcgaatgga
     2701 cgtgaggcgc tcgacgatct cctcatcgtc gagcccgcga agacgggccg acgtggggac
     2761 ggtgccgtcg agggtcttcg cggcgaggtc gcggatggcg agcatcttgg cgcgcgagag
     2821 tccggcgccg cgcatcgctt cgtcggacgc cgcgagcacc ccctcgggcg tggggccggt
     2881 gccggggaag agcgcgtcga agcgtcccca gatcgtagcc gcggccttgc cgttcagctg
     2941 ctggtaggcg atcgactcgg cgagcgccaa aaacggactc ttggtcgagc gggtggcgtg
     3001 gaagccgcag tccccgaccc ggtccatcag cttggcgagc gtgggatcgg cgtcgcgcag
     3061 gtggcgcacg gcgtggtcga tgtagtcggg cgaagtggcg cgcttgggct tgggcatgga
     3121 gcccaagagc ctaccggctc cggtttcccc ggacaatccc gtatgatgcc cccatggcgc
     3181 gccggcttcg taccgacctg ccgttcaagc gaatcgcggt cgtgctctcg ggagggggcg
     3241 cgctggccgc gtatgagacg ggcgttctcc gcaccctgga gcggatcggg ctccgccccg
     3301 cgatcgtctc gggggcttcg gcgggcgcgc tgaacgcggt ggcctgggtg gcgcacgact
     3361 tcaagacggc cgcgctcgag cgcgtctggc gccatgtgga cgcggcggag atcggcatgc
     3421 gctggacgac cgtcgcgctc cgcgcggcgg gcggtcttct cctggtgctc ggcctgatgc
     3481 aggccctcgt gagcgtgacc ggctcctccg acttgagctt cctcgccctg ttccatcacg
     3541 gtgcgccggt ggggctctgg cccacgtcgt ccttcctcga catcatggcg tggctcctgg
     3601 tcgcggccgg cgggtacgcg ctgctccgct cctcccgcga ggcggagaaa tttctcgcgc
     3661 ggttcggaga ccccgactcg gagcgccggg tccggctgtg gggcgcgatc atcctgctcc
     3721 tctggtcgat cgcgcacttc acgacgtggc tcctggcctg gccctggccc catcgcttca
     3781 gcgccacgct gctggcggtg accgcgggcg tgtggttcgt gacccgctcc cagcgcgcag
     3841 gcgccatcgc ccgccgcgtt ctcttccttc tcatgcccga gacggacggc cgcgggatct
     3901 gggggagcgc cgcgcgccgg cgcctgctga ggcgcctcat cggggtccgg cgcctggagc
     3961 ggctctggcc gggcgagacg catctcatcc tgagcgcgct cgcgctggac aacggccgca
     4021 tcgcccattt cgtgaactgg cccgcgacca aggcgcgctt ccatgcgcgg gtcgagaccg
     4081 ccctggggga agtgatcccg ctggtgggtc cggaagaggt ggtggaggcc gcgatcgcct
     4141 cttctgcgat tcccgtgctc ttcgagcccg cgcggatcgg gggacgcgat ttcgtggacg
     4201 ccgtcgcttt ctcgatgcac cccctgcggg ccgccatcta cgacgatgcc gacgccgcgg
     4261 tggtgatcgt cgtggccccg agcggcgctc cgcccgcgcc caggccgcct cgaaatccct
     4321 tcgaagtctg ggggcgctac ctggatctcg ccaactggcg cgacctgcag caggagctgg
     4381 tctccctgcc cggcgactgg catagcaagg aggcgccgca tgcgctctgc ctggtggaac
     4441 cggaagcggc gctcccgggc ggaacgctcg cgtattcgcc caagatcgct gcgcggctga
     4501 tccggcgcgg ggagctggac gcgctgcggg cgctcgatcg cgcggggtgg ctcgagacgt
     4561 agatgccgct cctcactcgg gaatggccgc ggctgcccga ggcgcgccgc ggctacgtgg
     4621 acgccatctt tcgccggatc gctccccgct acgacttcct cacgcgcgtg ctctcgttcg
     4681 gccaggaccg ccgctggaaa gaggccgtga tcgcgcggct gcccgcgccg cgcgagaacg
     4741 gcgtcatcct cgacctggcc acgggcacgg gcgagctgcc gctcctggcg cggaagcggg
     4801 gccgtcccga gcgcgtggtc gcggtggatc gaagccgctc catgctcgag cgggggcgcg
     4861 tcaaggtggc ggcggcggga gcggaccgga tccatttcgt ggcgggagat ctgaacaccg
     4921 tgccggtcgc cgagaaggcg ttcgacgccg tgctcatcgg ctacggcctg cgctatccgc
     4981 aagacctgcg ccgcgtgctc gcggagatcc tccgctcgct ccggccgggc ggcgtcttcg
     5041 tgtcgctcga tttcggggtg ccgcggaacg gcttcctgcg ccgcgtgatg ttcggctacc
     5101 tgctcgggat ggggaccgtc tggggcgcgc tgctgcatgg aaagccgggc acctactggc
     5161 acatcgtgga atcgctgcgg gcctatccgg gacaggcgcg gctggcggag ctgatggagg
     5221 aggtgggcta cgagcgggtg cgcgtggagg agcggctgtt cggctcctcg gcgatcgtga
     5281 ccggcgtgcg cggcccggtc acggcttgag gaagcggtag acgagccgtt tcacctgctc
     5341 actggccacg agccggattc ctctccgggt gcgaaaccac cggccgggcc catgcggatc
     5401 ggcgccccgg gaaggcgtcg cgtagacgac ggcgacgccg cgtccggcga gcgcgcggcg
     5461 gacgacccat cgcacgcgcg ccaggtggaa cttcgaggtg atcacgagga ggttcctcca
     5521 gccccgctcg gcgaccaggc ccgcgagcgc ccgcgcctcg ttcctggtgc cccagcggcg
     5581 cggtgtcacg tggcgcacct cgacgttgtt tcgctccagg atcacgaaga agtggcgttg
     5641 ttccggcggc acgacatcga cggcttcctg cagcgcgtcc acgccgggaa gccccaggag
     5701 cgcatagcgc cgccactcga agcgggccac gctgacgacc agcgccatct ccggcgcgtc
     5761 cgacgcatgc atgagcacct gccagaggcc ggccgcgcat tccttgcgcg cctgcccccc
     5821 cgcgaacacg aagatggcgt cggggcgagg gcctttcgcc ggaggcggat cgggcaggcg
     5881 cgatccggcc atgctcagcg gagcatcagg tagagcgacg ccgccgacgc gacggcgccg
     5941 agcagaagtc ccgccgccgc gatcgttcgc acggcgcccc gcccgacggc cagcgcgatc
     6001 cccattttga ataccgtatt cgaaagaacg cccacggcga tcgcctgcgc ggcgatcagg
     6061 agcatcgagg gatcttccgc ggtgcgcgcc atcgagatcg tgagcgcgtc cacatcggtc
     6121 aagccgagca ccgcgccgga ggcgaggaga cccgcgccgc cccacagcgt gcgcacggcg
     6181 tgcacggcga agagcaccac ctggaagatc gccgccatct ggagcgcgct cttcagctgg
     6241 agcgggttcc tgggctcggg catcgaggcg tggttgtcgc gcgcgcgccg gatcccgatc
     6301 agcgtcacga gcacggtgac cgcgaagggg gcggccaggt agggaagtgt ctgggtcatg
     6361 agatcggtgt tcagcacggc tgtggccacg acgacgcgca cgtagagcag cgcgcaggcg
     6421 gcgaggatgc caaaggcgag cggcggcccc gcttcgggat gctcgcggct ctggcgcgag
     6481 aaggtgagcg tggtgctggt cgaggagatc agcccgccca gcagtcccgc caccaggtag
     6541 ccgtgcttct cgccgatcac gcggcgggcg atgaatccga gataggagag ccccgagaag
     6601 aagagcacca ggatccagag ctggcgcggt cggatgccgc cgagcgggcc gtagggcccc
     6661 tgggggagca gcgggaggat cacgacggcc atgacgagga actgcgcggc cgcgcggacc
     6721 tcgcgggaat ccaggtggct cacggcatgg tgcaggcggg acttctcgac gaggaggagg
     6781 gccgtgaccg cgatcatgcc gctcgcgagc accagccggc cggcgcccgc cagcacgccc
     6841 gccgcgatca cgacgagggc ggcgacctcg gtggtggcct cgacgtcttc gcgatggagc
     6901 ttccagtagc cgcccgccac gaccgcggcg ccgccggccg cgagcacggc acccagctcc
     6961 ggggcttgga gcgcccagca gagccccgcc gcgccggcga ggagcccgag aagggtgaag
     7021 gtgcggatgc cgccgaaccg ggcgcggggg ccggtcgcgt gccccgacca ctggcgttcg
     7081 atcccgatgg cggcgccgcc gagccctgcg atcgcgacgt ggagcgcggc ctccgcctcg
     7141 ggggacatgg accgactcta gccgacaggg gggagcgctg gaagccgtct cgtctcagcg
     7201 tgtctccgtc acaagacagc tgtctttgct ttcacacgcg ccagcgcggg caaatatcga
     7261 tggagactgg aatgccagtc ttcataggga agctagaagg gcgaaggcgg attcgaactt
     7321 ggcgatctct gaaacgccac cttctccgcg ggggcccggc gcagcacgcg cgggagcgcg
     7381 ccggcgatcc agaccagcca ggcgaggatc catgccgatc cgctcaccga gagcatcgcc
     7441 atgcgcgccg agggcaggag ctccgcgagg agccgcgtga ccacggccgt cgcgagaagg
     7501 agcgccatcg caggccgaag cacgaaccct tcctggacga tcgggtagct tccatggcgg
     7561 atcacgaccc gcgtcgcgat gccggccgag agcgtcccga atccgccgat gaagagcgtg
     7621 tgcgctccga gaagcggaag atggggcgcc agggccatga gccagagtcc cgcgaagatc
     7681 atccaccccg atccccacag gaagtaggag acgcgatcgt ggcgtccagg cagacggaac
     7741 agcttccaca ccaagagaag cacgagggaa ccggtgaccg cgcggaccaa cgctccagcc
     7801 tgcagccggc cggaagcctc gagcagcaag gaccccagca tcgcgagcgc gatcagcgca
     7861 tagagaatgc gccgcggctt ccgcacgtgc ggcttcgcga ttccggggat gacgagggga
     7921 tcgcgcatcg acgtgaacgt gggcaccagg agcccgccca cgcccagcac gatggagagg
     7981 atcatcccca tccagatgag gcggagcccg aagcgaggcg agggctcggc ccaggcgccc
     8041 agcgcggcgc cgccggccat gatcgcgccg gcgaagccga ggaggagccc gaacgccacg
     8101 agcaggaact cctcgggcgg aggcgacgtg ctcttgctca cacggcggag cgccatcacg
     8161 acgaccaggg cgagcgtggc cacgtagaag agctgcacgg cgaggatcga accgagcagg
     8221 gccgcggtac cgacgccgag catcaggacg gtcgcggtgg ccagttccca ggggcggctg
     8281 gggagggagt gcatccacgc gggcatcgag gtgagcagga agcccaggat gaaggcgtgc
     8341 tggaagccct ggatcatcag tgtccagtgc gtcacggccg gataggggat caggccggcc
     8401 gcgtggagcg gccagagcag cgcgcccacg accccgtaga gaacgccgag cggaaagagc
     8461 acgcgatagg ggtcgattgc ctttcgagcc gctgttgctc cgggtgcggc cgcccgcggg
     8521 acgggcgccg ggcggagggg tgtcgccacg gccctcacca gtcccggcgg ccgaagacgc
     8581 gggccgccac ggcaagggga accgcggtct cgacgaggag cacggccagg ccgaggagcg
     8641 cgccaccgcc gccgcccagg agctttacga gcaccgcgga ggtcggtccg aagagcgctc
     8701 cggagccgac ggccagggtc acgaggacgc gggcgagatc caccgggttc agcaataggg
     8761 ccggaaagag caccgactgg agcggcacgc cgcgcaggag cgccgtggcg ccgagaacgg
     8821 ccagatcgta ggcgatcacg aggagcagcc agaggaacac cgcggcggcg agcgccttga
     8881 cgcggtcggc caggatcgag gcgagcgcga gcgcggccgc gagcgacagg gatcccagga
     8941 gcagcgacac ggcggtgagc gcggcgaagc cgcggatctg ctcgggtccc gcctggaggg
     9001 ccacgatcag gccgcccgcg ccaaacccga tcgcctgcgc cgcgcccagc gcggcggtca
     9061 ggccgaggta cttgcccgcg aggacctcgc cgcggccgac cggctgcgcc agcaagagtg
     9121 gcagtgtctc cgcgctgccc gacacgctgc ccactccgag caccagtccg gtcaacggca
     9181 cggtgaagag caccagattc aagaggctca gcgtgacgcg ggagaagccc tggaagccga
     9241 cctcgccact ctgcacgaga ccgaagaagg agacgccgag gacgagcgct gcgaagagcg
     9301 cggtgaaggc gaagagccag cgggtttcga gcgcgcgccg gtactcctcg cgcgcgatca
     9361 ctacgatacg gcgcgggcgg cccggaacgg agggcgttgc gacgggggcg acggcggcga
     9421 tgggtgcggc gttcatcgat gtccctccac caggtgggag ccctgtacga ggaagacccc
     9481 ggcgggcttc ccgtcgagga gccatgcgaa gtcgcttccc ggacggtccg cggtgaatcg
     9541 gaccgtcgcg gcgcctcccg gcgagagcgc ggcgggggat accgcgctct catagccgag
     9601 aagcgaaaac tggtgcgcgg acggatcgcg gttttcgacc cgcaccagga ccgcggcgcc
     9661 cttgggcacc tcgatgcggg ccgggagcac ggcgccgccc tggaccacga gcgaaagcgt
     9721 gcgctccgcg cgcgcattgg cggggacggc gcgcggcgcc acggcggcgg agcggggccg
     9781 cgcggccgcg aggatcagcg ccgcgccggc gaggacgacc aatgcaagga ggccatagcg
     9841 cttcatgggt ttgactcggg cggcgcgttc cgcgcgtcgc gctccttccg caccgccgcc
     9901 aggaagatct cttccagcgt gggtcgcgtc agctcgaagg tgcggatctc cacgccggcc
     9961 tcgcgcagcg cttcgagcgc ggcgacgccg cgtccattcg ccccctcgat ggcgacgcgc
    10021 cgctcacccc agggggtgaa cgcgatgccc cggcgggcca gggcggcacc cgcggcctcg
    10081 gtgttccgcg cctcgatctc gagccgcgcg ctggaggcct gccgcgcgcg aaggtcggcc
    10141 agcgattcca gcgcgacccg ccggccgccc gccaggatca gcacgcggtc ggccacgcgc
    10201 tcgacttcgg ccaggagatg cgaacagagg aggatcgtct tgccgtcctg gcgcagccct
    10261 tcgaccaggg agcggaactc ccaggtggcc tcggggtcga ggctggccgt cggctcgtcg
    10321 agcacgagga gcgacgggtc cccgagcagc gcctgcgcga gaaggaggcg ctgcgcgtag
    10381 cctcccgaga gctccgaggc gcggcgtccg gccagggcgg tcatgccgac gcgatcgagc
    10441 gcgcgggaga cggcgtaccc gtccaggccg cggagcgcgg cgaaaaagcc cagcacctcg
    10501 gccgcggtgc gccccgccgg gagatcgacc cgctgcggca cgtagcccat cccgcgccgc
    10561 gtctcggccg catgcggcgg aaggcccgcg aggccgaccg cgatcgagcc ggaggtcggg
    10621 cgtgccagtc ccaggatcag cttcaacgtc gtggtcttgc ccgcgccgtt gggtccgatc
    10681 agggcgaagg tctcgcccgg ctccacggag aaggagagcc cgtccaccgc cgtgacggcg
    10741 ccatagcgct tcgtgacgtc atggagttgg atcatcgggc cgccttgcgc cgggcgatac
    10801 cgatcacgcc cgctcccgcg aggagaagga acccggccgc ggcgggatga acctcgcggt
    10861 ggaccgggcc cgctgaagac gaggcggcgg gcgatacgcc cgccgccgag cgtgacccga
    10921 cgcccgcgcc ggcggggcgg accagggggt aatggtccac cgcttcgctc gggcggagcg
    10981 ccgggatcgt gcgctcggca agggagagcg cccccgcagc ggggctctcg ccgaagatcg
    11041 ccaagtcggg atactgcttg gagaggaagg cgaacgcgct cacgggggcg tagggcacgt
    11101 cgccgatgcc gtcggcgttc agatcgtagg gggcgctcgc gctccagtag ttgccgcgcg
    11161 cgccgtcgtc gaaccggttg tcggtacgcc gcatgtccag cgccaccggg tagtcgatgt
    11221 tcacgaagtc gttccccgcg aactcgtttc cgtcgcagga ggagaagagg atgacgcccc
    11281 agccgtcgtc ctggaccaga ttcccatgga tgcggttccg gttggatcca tccatgaaga
    11341 ccgcgatcgt gttgtcgacc aggcggtttt cgtggaagaa gccgtccgag cagtcgcgca
    11401 gcaggatgcc gtacgtccgc gggccgcggt tgtgcatgaa gtcgtttcgg gcgagctcca
    11461 agtggttcga gaacatgatg gcgcagccgg cgatgttgcc cgtgaagcgg ttcccgacga
    11521 tccgattgtc ctggcagtac atggtgtgga ggccgtagcg gccgttgccc gaggagccgc
    11581 acccctcgat gcggatcgaa tcggcgaacg aaagatagat cccgtccatg aagcgccgga
    11641 tctccgttcg ccggatgcgg gcgccgcggg agtaccagac gtggacgccg ttgccggcgg
    11701 cgacgtccat ctcgggagcg gcgtcgaccg agggaacgac gcggccggtc agcgagcaat
    11761 cctcgacgtc caggtcgtcg gagcgctcgg cggcgacgcc gtagcgcacg tcttcgatcc
    11821 ggagtccggc gaggcgcgcg ccgggggccg aagacagccg gacgccggcg tccaggtcct
    11881 cgaaccggtc gccgctccgc cggagcgcga gccgttcgat ccggacgccg ggggccagga
    11941 ccgtgatgat cgatccgcgg ccgccgccgt ccacgaccgc gcccgggacg ccgatcagcg
    12001 cgacgcggcg cgtgaccacg agcggtcccg ggtggacacc aggctcgaga agaagcgtgt
    12061 cgccgtcgcc ggcgcccagg agccgcgcgg cggcctggcc tggagccacg cggatcgtag
    12121 acgccccggc gtgcggggaa gccgcgccca tgacgaggag gagcgtgagc gccacggccg
    12181 cgaggagggc gacgagcagc gcgatctgaa gtgcggacgc gagccgctcc gaagcgcgcg
    12241 gattcatgcc gccaccgccg ccgccgccgg cgcgtgggcg cgccgatcgg cgcggcgctc
    12301 catccagagc gcgagcggcc ccaggagcca ggcgatcccc agcacgagcg tgcccgggcc
    12361 cgggaggctc agcacgtgga agttggccat cttggaatag ccgatcaccg ggggcatgaa
    12421 cgccttcatg gtgatcgccg cgtgcgggtt cag
//
LOCUS       DASSLZ010000275        18443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1100194, whole genome shotgun sequence.
ACCESSION   DASSLZ010000275 DASSLZ010000000
VERSION     DASSLZ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 18443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18443
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100194"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VFM00_10435"
     CDS             <1..1087
                     /locus_tag="VFM00_10435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7498707.1"
                     /translation="GSRRGGVFRALRRAQSWVLIPDGYVGWARAGARAAGRRIRAGGI
                     DALWTTSSPESAHVAGLSLKRRFRLPWVADFRDPWVGRVTYAPPSRWHDARHRALEAA
                     VVRAADRITVVSEAMAALYRDRYPDLAPERIVVLENGVDPDDWKRADAGGFPSTAEDA
                     ARDRGRFVLLHAGQLAHRPTARTLLEAARRVIAADPALAGSLRVRFLGGNEEIAAGDP
                     LLRALGDVVALDPSRPHLEALAAMRRADALALLGHGGRADAFLYTGKIYEYLTSGRPI
                     LAILDAGPAAALLRESGHRGVFASADVAGVAAWLGAALGSWRAGAHAGPHAGGHRPDV
                     PDGHPGAWDRQFLAARAAEMLSRIVDA"
     gene            1289..1729
                     /gene="mraZ"
                     /locus_tag="VFM00_10440"
     CDS             1289..1729
                     /gene="mraZ"
                     /locus_tag="VFM00_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET7498708.1"
                     /translation="MAAFRGSYTHTIDHKGRVSIPARYRRQLSGDADETFVVLRGLDA
                     CIALYPADEFRRLDERLRSRSFADEANRRYQRMMLVDSRDETLDAQGRVALPPRLIAH
                     AQLEKDVLVAGMLDHLELWNPELFEKYLQSSPRTYEEIAGELLL"
     gene            1777..2178
                     /locus_tag="VFM00_10445"
     CDS             1777..2178
                     /locus_tag="VFM00_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7498709.1"
                     /translation="MLQWEAASGLASATLRLYGSLGRRELALVVETIAERARSPRDVV
                     SIDFENVAHLDFRALSEFAQKIARHRHRGASLWLVGLSPYVRSLFDVAGQGSVVRQLA
                     WQPEERIRREPIRAFARSGVDGASGADRFPR"
     gene            2193..3119
                     /gene="rsmH"
                     /locus_tag="VFM00_10450"
     CDS             2193..3119
                     /gene="rsmH"
                     /locus_tag="VFM00_10450"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET7498710.1"
                     /translation="MPGHVPVLADEAVRLLVTEPRGTYVDATLGGGGHAAKILAALGG
                     EESKVIGIDCDPAAVARAQANPPAVAPRFVAAQGRFSTLEATLKAHGIERVDGILADL
                     GLSSDQLDDPARGLAFAKEGPLDMRLDPSRSETADRLIRRIGEPELARLLSEYGELPR
                     AKTAARVIRRAAETERPLTTGGLSTALAPLYPGPSRPRRLAQAFQALRIAVNDELGEL
                     QALLDAAARIVRPGGALVVIAYHSLEDRMVKRAFSPARPFDPWLPPSEAPASPWEPLT
                     RRPIRPSAEESARNPRAKSALLRAARRRGGME"
     gene            3116..3655
                     /locus_tag="VFM00_10455"
     CDS             3116..3655
                     /locus_tag="VFM00_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498711.1"
                     /translation="MTHVAVVNEAVAADRELSRPAPKSAYLRRVRGAERRAQARPPLP
                     LVIGVAVVTALLFVRVWQVTSAHALAMEKDRLRKEVAALDNRIHLSSDLAIQEALHQG
                     LDYAALEKQGFQSPSPDALVDVDLAQPFPRVVSRQGAMARLSADVGRFVRGILPARRA
                     APRTPDEPRVLPVNAEVAP"
     gene            3652..5742
                     /locus_tag="VFM00_10460"
     CDS             3652..5742
                     /locus_tag="VFM00_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET7498712.1"
                     /translation="MTPRDAGRLRALALAGIFAFAVLLLRLVQIQVVDHGRLAREATA
                     QQTQRVILEPERGLIFDRHLRPLAENVELSQISVRPREVENAAAAAAFIKKAAGSEAV
                     SRFRAGRVRHRMYVRVSQQLRPEQELALTTSALPRGVHVDPVPGRVYPLDDVARSVVG
                     VVGHEGSGLEGLEAVYDRDLKGTAGWATLFQNGRGLAYELPGSMVKLPEAGASLVSTI
                     DLDAQTLTVMKLREAMATSGAKSAMAVFVDPNTGDILAMATVDGPNVDKRTGHRNRIV
                     ADQYEPGSTFKVLAGCAALEEKVFDPEDSIFVDHGQVNLGGFTIHDSHPETGWFTFHR
                     ATAHSSNVCFAQIGMRVGSERLYRYARLFGFGQPTRVTLPGEAPGQIRPPSRWSARSL
                     ATISIGQEVLVTPLQLVMAYAAVANGGTLLRPRLASALVDENGKVVRQVPIERVRRVI
                     SEETARTFRSFLRETVVSGTGTEAALPWCDVAGKTGTAQKFDAGAGGYRGGRYTSSFL
                     GMAPAENPRVVGLVIPDEPRGAYYGGSVAAPVWREIVAAWAAQGHGPIALPAAVLPPV
                     AFAPAPSADPVPDVRLLAADRAADILERAGYAPKILGDGGRVAAQSPAPGAVVPSGAV
                     VELTLASESPAEAVVPDLRGLPIRDAVARLSALAIPVGRVVGTGSVVNQNPEPGRPVR
                     PDTRCSLTLSPRGT"
     gene            5709..7256
                     /locus_tag="VFM00_10465"
     CDS             5709..7256
                     /locus_tag="VFM00_10465"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET7498713.1"
                     /translation="MFPHAVPPGDVIASGIREAAALPRIVGALEMRGAPAGEWTRVQY
                     DSRAVQPGDVFVAIPGEKADGHGFVPEAAKKGALVAIVERFTSEAAWPEVRVKSARRA
                     LAILAGEETDHPSHDLLVVGVTGTNGKTTTTHLIRAALKERGERVGLIGTVGYELEGD
                     HEAAPHTTPEAPELARLFRRWSDRGASGVVMEVSSHALAQDRTYGIAFDVGVFTNLTQ
                     DHLDFHGTMEAYRDAKARLFRAETRGDRTKTMAGVLNMEDEAGRWIRERADGPTIGYG
                     GEGAVGPGDVGAAEVVAEEARLGPAGTRLRIRYPRGSVSVALKLRGRFNVANALAAFA
                     AAYAAGTPPEAIARGLESVASVPGRLEPVDAGQPFQVLVDYAHTPDALERALEAVRAF
                     GPHRILCVFGCGGDRDRGKRPLMGAAAARLADRVFITSDNPRSEDPEAIVREIEGGAH
                     GTASVATILDRAEAIRAAVDEAQAGDALIIAGKGHETYQILSTGTIPFDDRTAAREAL
                     RARGFSG"
     gene            7253..8695
                     /gene="murF"
                     /locus_tag="VFM00_10470"
     CDS             7253..8695
                     /gene="murF"
                     /locus_tag="VFM00_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET7498714.1"
                     /translation="MTAAARGWLTLEGVLRAVDGTLASPAGTTLAAPRDLEFAGCAID
                     SRTLQAGDLFVPLPGTRADGHEFIAAALQGAAAGSLIGRDRPVPPEAAATKKPVIRVA
                     DPLGAMQALGRWCRDRAGIPVVAITGSNGKTTTKEMTAAVLGTSRRVHKNVGNLNNHI
                     GVPLTLTRLRPEHEALVVEMGMSARGEIRDLAAMAQPTIGVLTNASAAHLQQLGSVEE
                     VARAKSELAEALPTQGLLVLNADDPLLYPMNRERIVRKTTYGLDNPDAALRPTRVLVD
                     EDGTTRFTLEDGSEGRLSLLGRHNVRNALAAIAVGDELMVPRDAALAALAALRPAKHR
                     LEILHAGGVAVIDDAYNANPASMREALAILGGVAVEGERRAVLGDMLELGPGSEALHE
                     TVGRAVPAGAWLYVAGSFAAAIERGAKAAGVAAARIRRFEDVDAMAAAVAADARAGDL
                     VLVKGSRGMRLERVVQALQAAGAQAGAGRD"
     gene            8699..9787
                     /gene="mraY"
                     /locus_tag="VFM00_10475"
     CDS             8699..9787
                     /gene="mraY"
                     /locus_tag="VFM00_10475"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET7498715.1"
                     /translation="MFYHLLYPLHEHFAPFNVFRYITFRAAYALATALFIALFLGPPV
                     IRRLRALKIGQKVRDDGPQSHLPKAGTPTMGGVLMVISIAVTTLLWGNLSNRNVWIAL
                     IATIWMGTVGFIDDYLRVVKNFRKGLLGRYKLAGQITLGIGVFAAVFFYPAHGLLDPA
                     ETNIPFLKRTVVDFGWLYLPFVILVITGASNAVNLADGLDGLAAGLAAFGAVALGGMC
                     YITGHAKFSQYLQVPYIAGTGELAVFCAAIIGATLGFLWWNCHPADVFMGDTGSLSLG
                     AALGTVAVLIKREFLLAIVCGVFVIEALSVMAQVLSFKIWGIRVLKMAPLHHHFELSG
                     WKETRVVVRFWIAAALCALLALSTLKLQ"
     gene            9784..11175
                     /gene="murD"
                     /locus_tag="VFM00_10480"
     CDS             9784..11175
                     /gene="murD"
                     /locus_tag="VFM00_10480"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET7498716.1"
                     /translation="MSTTTQPWFAGSKALVVGLARSGIAAARLLRRHGASVRAVDRRR
                     AEELSPDAADLESLGVELRAGGTDPASLEGRDLVVVSPGVPLDLPLFREAERRGIPIA
                     AEIELGFAVAKAPIVAVTGTNGKSTTVELLGAFGRAAGRPTEVLGNIGTALSERAESV
                     PEEGLLVVEVSSFQLETCTRFRPDVGVILNVTPDHLDRHGTLERYAAVKARLFAHQTD
                     RDYRVQPLGDPLLVSLLAPMRSRPLWFGFTDPTADGVWEEGGALRSRFEGREGTIVKR
                     SDAKLEGPHNTENMAAAAAAALAVGISERAIASALRQFRGLPHRLALVAEIDGVRYVN
                     DSKATNVDSMKRALESFGDAPITLIAGGRDKNGDFASIAPLVAEKVRRALYVGEAAPI
                     LERAWPHLSAQRAATLEDAVRLARAETPAGGVVLLSPGCASYDMFRNFEERGARFEAA
                     VHALQRDPARAKP"
     gene            11172..12344
                     /gene="ftsW"
                     /locus_tag="VFM00_10485"
     CDS             11172..12344
                     /gene="ftsW"
                     /locus_tag="VFM00_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET7498717.1"
                     /translation="MNVALDRARRPDFKLLVPVVCLVALGLVMVFSSSASLAAHRYNS
                     EYFFLKRHFVRVLIGVALLVVVARTDYHVLQRLAAWALGGTVLLLGALLALGSAVRGA
                     NRWLSLATVTIQPTEIARVACVVYLAKLLDRKGERMASFKEGVLPACMVLGLMAFLIL
                     LQPNLGSTIALLSTGFVMLHLSGARRAHLALLALAGVLVAVLAVSHNGYMMERVQGWL
                     AQWGHGRSDSLGKNWQVSQSILALGSGGVLGAGPGHGLQKVFFLPDPHTDFIFAVIGE
                     ELGLLGTAGVLAAFSVLFLRGLKVAAGAPDRFGYLLAAGLTVNLSVYVAMNIAVVTGV
                     IPTTGLPLPFVSYGGSALVVNLGVIGVLLNIAHQMETGYRTSAVVRPPGVGRRRVR"
     gene            12250..13470
                     /gene="murG"
                     /locus_tag="VFM00_10490"
     CDS             12250..13470
                     /gene="murG"
                     /locus_tag="VFM00_10490"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET7498718.1"
                     /translation="MFSSTSPIKWRQGIGRPRSSGRPESDAGASDRVAPLRLLIAAGG
                     TGGHVYPGIAVAEEWRRRHPDSEVVFVGTARGVESTAVPQAGFALRTIAARGIPRRLG
                     FGVIRAVAAMIQSFVQAQSLLRDLKPQVVVVTGGYVSGPVGLAAKLRGIPVVVQEQNS
                     VPGATNLWLNLIADEVHISFVESRNYFRRRNNLKVTGNPIRRSLLRQDRTSAYEFLRL
                     DPHRSTLLVFGGSRGATSINRAFQDALPRLTRLQNIQVLWQTGKDEAAAIRDRVKSLS
                     IPVHVLPYLDQMEKAYAVADLAVCRAGAMTIAELTACGVPSILIPYPHATHDHQTVNA
                     RGLMERGAAEVIRDDELEAEDLAKRIVQLFQDEPRLRRMARNARAFSRTNAAERLVTS
                     IESLAGAVPAERMG"
     gene            13473..14867
                     /gene="murC"
                     /locus_tag="VFM00_10495"
     CDS             13473..14867
                     /gene="murC"
                     /locus_tag="VFM00_10495"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET7498719.1"
                     /translation="MYGRIRRIHLVGIGGSGMSGIAEVLLNLGYEVTGSDPKEGEAVA
                     RLRDIGARIASTHDAMHVVGADVVVVSSAIPETNPEIRRARALGIPVIPRAEMLAELM
                     RIKTSVAVAGAHGKTTTTSMVGEILANGGLDPTVIVGGRLKTVGAHARLGQGPFLVAE
                     ADESDGSFLLLAPTIAVITNIDAEHLDYHKSLEGVQLAFTAFANRIPFYGTIILPGDD
                     PNAAPIRARAKRRALTYGFTPGLDFEGKNLDVGPAGVRFDLLVRGESEGSIELSVAGA
                     HNARNALAALAVGWELGVPIAAIRGALRSFAGVGRRLERRGDLLGALWIDDYGHHPTE
                     IEAAIAALRATYERRIVAVFQPHRYTRTHALADRFATAFRGAAELVLLPIYPAGEAPI
                     PGVTAESLAERIRGGSGVTVRYAADYDDAARVARDLVRDGDLFLTIGAGDVFKVGEII
                     LAEGENAPAAAGRA"
     gene            14864..15715
                     /locus_tag="VFM00_10500"
     CDS             14864..15715
                     /locus_tag="VFM00_10500"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET7498720.1"
                     /translation="MRAGYRALPSWKKPAVKRRRRRIVMLLALSALATLTAPLAVKPA
                     ARALGSLQPFQVRSIEVSGLLYLAPEEIRSSVAQAGARVGDNLLLVSPARVEAGIEKN
                     ARVERAQVLRVPGKLIVRVTERRTFVLVSAGSLLEVDADGTVLSPLKRGLVADRPIVT
                     GLKIRPAAPGSKLSGERMQDLLRLVRLLESPEVGLVSEISEIASDAPARAVLRTSRDQ
                     IPIVVDPERVTLAAMRAVAATLRDVRERDRRVLVMDARYRGQVVVRCGPDSSTGARTG
                     VDPRAKV"
     gene            15739..16965
                     /gene="ftsA"
                     /locus_tag="VFM00_10505"
     CDS             15739..16965
                     /gene="ftsA"
                     /locus_tag="VFM00_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET7498721.1"
                     /translation="MEAKIYAGLDIGTTKITAIVAEPDENGESLRIIGVGTAPSDGLK
                     RGVVVNLEKTVRSIQHALEEAERVSGRSIKSVWAGIAGDHIRGLTSRGVIAVSRKDAE
                     IRQNDVARVIEAAKAVAIPADREILHVLPQEFIVDDQEGIRDPVGMSGVRLEAEAHII
                     TGAAAACRNVIRAAERAGVHVEELVLEPLASANAVLAPDEKDLGVALLDIGGGTTDVA
                     VFFEGAIRHTAVIGLGGSNVTNDLAIGLRTPVERAEILKLGSGCALTSMVRPDETVKV
                     PSVGGRPDRDISRHMLAMMIEPRVEEIFAMANREIRKSHVADLLGAGVVLTGGASSLP
                     GMPELAEQVFDLPVRRGIPTGVTGMVEAVSDPRFATGVGLAQHAFWNDASPHVAGEGL
                     FGRISIGIRRWIEELV"
     gene            17042..18337
                     /gene="ftsZ"
                     /locus_tag="VFM00_10510"
     CDS             17042..18337
                     /gene="ftsZ"
                     /locus_tag="VFM00_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET7498722.1"
                     /translation="MFELVDEGGERSGASIKVIGVGGAGGNAVNRMMESGLTGVEFIV
                     ANTDAQVLVQSRCPRKIQLGTAITKGLGSGANPAVGRQSAEADEEVIQEALSGSDMVF
                     VTAGMGGGTGTGAAPVVARIARSLGALTVGVVTKPFEFEGRRRAQSADEGLRELRENV
                     DTLIVIPNQRLLAIVEKNTPLRDAFKTADQVLHHATKGISDLITVPGLVNLDFADVKT
                     VMAERGNALMGAGYATGPTRAYEAAQSAVSSPLLDDISISGAEALLVNVTGGESMTLH
                     EISEAVSVVVDAAGHEANVIFGAVIDDRMGDGLSITVIATGFGRAEAKMRPATIPMGA
                     RTPVARPQAAPVAASAAAPAQIFELEQRPAMKQVRAAMEELEEEENDAVETPEPAPAA
                     SNGRPSFRIPPGTARRPFGGRAITRDNMDVPAFIRKQMD"
BASE COUNT         2541 a         6453 c         6798 g         2651 t
ORIGIN      
        1 gggatcgcgg cggggcggcg tgttccgcgc gctccggcgc gcacagtcgt gggtcctgat
       61 ccccgacggc tacgtcgggt gggcccgcgc gggggcgcgc gccgccgggc ggcgcatccg
      121 ggccggcggg atcgacgcgc tctggaccac gtcaagcccg gagagcgcgc acgtggcggg
      181 cctttccctg aagcgccggt tccgccttcc gtgggtcgcc gatttccgcg atccctgggt
      241 ggggcgcgtc acctatgcgc ccccgtcgcg atggcacgac gcgcgccacc gggcgctcga
      301 ggcggcggtg gtgcgcgccg cggaccggat caccgtcgtg agcgaggcga tggcggccct
      361 ctatcgcgat cgctaccccg atctcgcgcc ggagcggatc gtcgtgctgg aaaacggcgt
      421 cgaccccgac gactggaagc gggcggacgc gggcgggttt ccgtccaccg cagaggacgc
      481 cgcgcgcgac cggggccgct tcgtgctgct gcacgcggga cagctcgcgc atcgtcccac
      541 cgcccgcacc ctgctcgagg cggcgcgacg ggtgatcgcg gccgatccgg cgctcgcggg
      601 gtcgctccgc gtccgcttcc tcggtgggaa cgaggagatc gccgcgggcg atcccctcct
      661 ccgcgccctg ggggacgtcg tggcgctcga tccctcccgc ccgcacctgg aagcgctcgc
      721 cgcgatgcgg cgggccgacg cgctggccct gctggggcac ggcggccgcg ccgacgcgtt
      781 cctctacacg gggaagatct acgaatacct caccagcgga aggccgatcc tggccatcct
      841 ggacgccggc cccgcggcgg ctctgctccg tgaatcgggc catcggggcg tcttcgcctc
      901 cgccgacgtg gccggggtag ccgcctggct cggggccgcc ctcggctcct ggcgtgccgg
      961 cgcgcacgcc ggcccgcacg ccggcgggca ccgtcccgac gtcccggacg ggcatccggg
     1021 cgcctgggac cgccaatttt tggccgccag agccgccgaa atgctgtctc gaatcgtgga
     1081 cgcctgaccc cttccctaat tttttcccca ccctctcccc ccggacttaa gccgaagcgc
     1141 cccaaccgaa tgaattttca atttttctgc gaatccggct tgacactccg gaatcccgag
     1201 gcctaaagtg ccgctcgtgg caagaagtgg gggtaagtgg ggaaacgtgt agctccaggg
     1261 acggagctgg tgggagggtc agggcgggat ggcagcgttc cggggcagct atacgcacac
     1321 gatcgaccac aagggtcgcg tgagcatccc cgcgcgttat cggaggcagc tttcggggga
     1381 cgccgatgag acgttcgtcg tcctgcgggg cctcgacgcg tgcatcgcgc tctatcccgc
     1441 cgacgagttc cgccgtctgg acgagcgcct ccggtcgcgt tccttcgccg acgaagccaa
     1501 ccgccgctac cagcgcatga tgctcgtcga ctcgcgggac gagacgctcg acgcccaggg
     1561 gcgcgtcgcc cttcccccgc ggctcatcgc gcacgcgcag ctcgaaaagg acgtcctcgt
     1621 ggcgggcatg ctcgaccacc tcgagctgtg gaatcccgag ctgttcgaga agtatctgca
     1681 gagctcccct cgcacctacg aagagatcgc cggagaactc ctactgtgat cgcaccgctc
     1741 cattccgcac gacgtccggc cgcgagcgag cggccgttgc tccagtggga ggccgcgtcc
     1801 ggcctcgcct ccgccacgct tcgactctac ggctccctgg gccgccgtga gctcgcgctc
     1861 gtcgtggaga cgatcgcgga gcgcgcgcgg tcgccgcggg acgtcgtctc gatcgatttc
     1921 gagaacgtcg cccatctcga cttccgcgcg ctttcggagt tcgcgcagaa gatcgcgcgg
     1981 caccggcacc ggggcgcttc actctggctg gtcggcctgt cgccgtacgt gcgcagtctg
     2041 ttcgacgtcg cgggccaggg gtcggtggtt cgtcagctgg catggcagcc cgaagagcgg
     2101 atccggcgcg agccgattcg cgccttcgcg aggagcggcg tggacggtgc ttcgggagcg
     2161 gaccgcttcc cgcggtagcg ccccgcgaat cgatgccggg gcacgtcccg gtccttgccg
     2221 atgaggccgt gcgtttgctg gtgacggagc cgcggggcac gtacgtcgat gcgaccctgg
     2281 gtggtggtgg acacgccgcg aagatcctgg cggcgctggg aggggaggag tccaaggtga
     2341 tcggaatcga ctgcgacccc gcggcggtcg cccgcgcgca ggcgaacccg ccggccgtgg
     2401 caccgcgctt cgtcgcggcc caggggcgct tctccacgct cgaggcgacg ctcaaggcgc
     2461 acggcatcga gcgcgtggac ggcatcctgg ccgacctggg gctctcctcg gatcagctcg
     2521 acgatccggc gcgcgggctc gcgttcgcca aggaggggcc gctcgacatg cggctcgatc
     2581 cgtcgcgctc ggagaccgcc gaccgcctga tccgccgcat cggcgagccg gagctggcgc
     2641 ggctcctctc cgagtacgga gagctcccgc gcgcgaagac ggccgcgcgc gtgatccgcc
     2701 gcgccgccga gacggagcgg ccgctcacga cgggcgggct tagcaccgcg ctggcgccgc
     2761 tctaccccgg gccctcgcgt ccgcgccggt tggcgcaggc gttccaggcg ctccgcatcg
     2821 cggtgaacga cgagctgggg gagcttcagg ccctgctcga cgccgccgcg cggatcgtcc
     2881 ggccgggcgg cgccctggtc gtcatcgcct atcactcact cgaggatcgg atggtgaagc
     2941 gcgcgttctc cccggcgcgc cccttcgatc cctggctccc cccgtccgaa gctcccgcgt
     3001 cgccgtggga gccgctcacg cgccgtccga tccgcccctc ggcggaggag tcggcccgaa
     3061 accctcgcgc caagagcgcg ctcctgcgcg cggcgcgacg ccgtggagga atggaatgac
     3121 gcacgtggcc gtggtcaacg aagccgttgc cgccgaccgg gagctctccc gtcccgctcc
     3181 gaagagcgcc tacctccgcc gcgtccgcgg ggccgagcgg cgggcgcagg cgaggccgcc
     3241 gctcccgctg gtgatcggcg tggccgtcgt gacggcgctc ctcttcgtgc gcgtctggca
     3301 ggtgacctcg gcccacgcgc tggccatgga gaaggaccgg cttcggaagg aagtggccgc
     3361 gctggacaac cggatccacc tctcctccga tctcgcgatc caggaagcgc tccaccaggg
     3421 cctcgactac gcggccctcg agaagcaggg cttccagagc cccagccccg acgccctcgt
     3481 ggacgtcgac ctggcccagc cgtttccccg cgtggtgtct cgacagggcg cgatggcgcg
     3541 cctctcggca gatgtcggcc gtttcgtccg aggcatcctc ccggcccggc gcgcagcgcc
     3601 ccgcacgccc gatgaacctc gggtcctgcc cgtgaacgcg gaggtcgccc cttgacccct
     3661 cgcgacgcgg gccgccttcg ggcgctcgcg ctggccggaa tcttcgcctt cgccgtgctg
     3721 ctcctccgcc tggtgcagat ccaggtggtg gaccacggcc ggctcgcccg ggaggcgacc
     3781 gcgcagcaga cgcagcgcgt catcctggag ccggagcgcg gactgatctt cgaccggcac
     3841 ctccggccgc tcgccgagaa cgtcgagctg agccagatct cggtgcgccc ccgcgaagtc
     3901 gagaacgcgg cggccgcggc ggcgttcatc aagaaagcgg cgggatcgga ggcggtcagc
     3961 cgcttccgcg ccggccgcgt gcggcaccgc atgtacgtgc gggtgagcca gcagctccgc
     4021 ccggagcagg agctggcctt gacgaccagc gcgctcccgc gcggcgtgca cgtggatccg
     4081 gtgccgggtc gggtctaccc cctcgacgac gtggcgcgct ccgtggtcgg cgtcgtgggc
     4141 cacgaaggaa gcggcctcga ggggttggag gcggtctacg accgcgactt gaagggaacg
     4201 gccggatggg cgaccctttt ccagaacggg cgcgggctgg cctacgagct gccggggagc
     4261 atggtgaagc tcccggaggc gggggccagc ctggtgagca ccatcgacct cgacgcgcag
     4321 acgttgacgg tgatgaagct gcgcgaggcg atggccacct ccggcgcgaa gagcgcgatg
     4381 gccgtgttcg tcgaccccaa tacgggcgac atcctggcca tggccaccgt ggacggcccc
     4441 aacgtcgaca agcggaccgg ccaccgcaat cgcatcgtcg ccgatcagta cgagcccgga
     4501 tcgacgttca aggtgctcgc gggatgcgcc gccctggaag agaaggtctt cgatcccgag
     4561 gactcgatct ttgtcgacca tgggcaggtg aatctcgggg gcttcacgat tcatgacagc
     4621 catcccgaga cgggatggtt cacgttccac cgcgcgaccg cgcactcgag caacgtctgc
     4681 ttcgcgcaga tcgggatgcg ggtgggctcc gagcggctct atcgctacgc gcgcctcttc
     4741 ggcttcggac agcccacgcg ggtgaccctg ccgggcgagg cgccgggcca gatccgccct
     4801 ccgtcgcgct ggtccgcccg ttcgctggcc accatctcga tcgggcagga ggtcctcgtc
     4861 acgccgcttc agctggtcat ggcctacgcc gccgtggcga acggagggac gctgctccgg
     4921 ccgcggctgg cgagcgcgct cgtggacgaa aacggcaagg tcgtgcgcca ggtccccatc
     4981 gagcgggtgc gccgtgtcat ctccgaggag acggcgcgca ccttccggtc gttcctccgc
     5041 gagaccgtcg tctcggggac gggcaccgag gcggcgctcc cgtggtgtga cgtggcgggc
     5101 aagaccggca cggcgcagaa gttcgacgcc ggcgccgggg gataccgggg aggccgctac
     5161 acgtcgtcct tcctcggcat ggcccccgcg gagaatcccc gcgtggtcgg cctcgtgatc
     5221 ccggacgagc cccgcggcgc ctattacggc ggctcggtcg cggcacccgt ctggcgcgag
     5281 atcgtggccg cgtgggcggc gcaggggcac ggccccatcg cgctccccgc cgcggtgctg
     5341 ccccccgtgg cgttcgcgcc cgctccttcg gccgacccgg tccccgacgt gcggctcctc
     5401 gccgcggacc gcgcggccga cattctggag cgcgccggct acgcgccgaa gatcctgggc
     5461 gacggcgggc gggtggcggc gcagtcgccg gcgccgggcg ccgtggtccc ctcgggcgcg
     5521 gtggtcgagc tcacgctggc ctccgagtcg cccgccgagg ccgtcgtgcc cgacctgcgc
     5581 ggcctgccga tccgggacgc cgtcgcgcgc ctgtccgccc tcgcgattcc cgtgggacgc
     5641 gtcgtcggaa cgggcagcgt ggtgaaccag aatcccgagc ccgggcgccc cgtgcgaccc
     5701 gatacgcggt gttccctcac gctgtccccc cgggggacgt gatcgcctcc ggcatccgcg
     5761 aagccgcggc cctgccgcga atcgtcggcg ccctggagat gcggggggcg ccggccggag
     5821 agtggacgcg cgtgcagtac gactcccggg ccgttcagcc cggcgacgtg ttcgtggcga
     5881 tccccggcga gaaggccgat gggcacgggt tcgtgcccga ggcggcgaag aagggcgcgc
     5941 tggtcgcgat cgtggagcgc ttcacgtccg aggccgcgtg gcccgaggtg cgcgtgaaga
     6001 gcgcccgccg cgcgctcgcg atcctcgcgg gcgaggagac cgatcacccc tcgcacgacc
     6061 tcctcgtcgt cggcgtcacc ggcacgaacg gcaagaccac cacgacgcac ctgatccgcg
     6121 ccgcgctgaa ggagcgcggc gagcgggtcg gcctgatcgg cacggtcggc tacgagctgg
     6181 aaggggatca cgaggcggcg ccgcacacga cgcccgaagc gcccgagctg gcgcggctct
     6241 tccgccgctg gagcgaccgc ggcgcctccg gggtggtgat ggaggtctcg tcccacgcgc
     6301 tcgcccagga ccgcacctac ggaatcgcgt tcgacgtggg cgtgttcacc aacctgacgc
     6361 aggaccatct cgatttccac gggacgatgg aggcgtatcg cgacgccaaa gcgcggctct
     6421 tccgcgccga gacccgcggc gaccgcacga agaccatggc cggcgtcctc aacatggagg
     6481 acgaagcggg gcggtggatt cgcgagcgcg ccgacggccc gacgatcggt tacgggggag
     6541 agggcgccgt aggccctggg gacgtggggg cggcggaggt cgtcgcggaa gaagcgcggc
     6601 tcggtccggc aggcacgaga ctccggatcc gatatccgcg cggctccgtc tccgtcgccc
     6661 tgaagctccg gggacggttc aacgtcgcga acgcgctcgc cgcgttcgcc gcggcctatg
     6721 ccgccgggac gccgcccgag gcgatcgcgc ggggcctgga gtcggtcgcg tcggtgccgg
     6781 ggcgcctcga gccggtggac gcggggcagc cgttccaggt gctggtggac tacgcccaca
     6841 cgcccgacgc gctggagcgc gcgctcgagg ccgtgcgcgc gttcggcccg caccggatcc
     6901 tctgcgtctt cggatgcggc ggcgaccgcg accgcgggaa gcgccccctg atgggcgccg
     6961 cggccgcgcg gctcgccgac cgggtgttca tcacgtcgga caacccccgc agcgaagatc
     7021 ccgaggcgat cgtccgcgag atcgaggggg gggcgcacgg caccgcgtcg gtcgcgacga
     7081 tcctggaccg cgccgaggcg atccgcgccg cggtcgacga ggcgcaggcc ggggacgcgc
     7141 tgatcatcgc gggcaagggc cacgagacct atcagatcct ctccacggga acgatcccct
     7201 tcgacgaccg aacggccgcg cgcgaggcgc tccgggcgcg ggggttttcg gggtgaccgc
     7261 cgccgcccgc ggctggctca cgctggaggg ggtcctccgc gccgtggacg gcaccttggc
     7321 ctcgcctgcc gggacgacgc tcgccgcacc gcgcgacctc gagttcgcgg gatgcgccat
     7381 cgacagccgc accctccagg ccggcgatct cttcgtcccg ctccccggca cgcgcgccga
     7441 cggccacgag ttcatcgcgg cggcgctcca gggtgcggcg gccggcagcc tgatcggccg
     7501 cgaccgcccg gttccgcccg aagcggccgc cacgaagaaa ccggtgatcc gcgtcgcgga
     7561 cccgctcggc gcgatgcagg cgctgggtcg ctggtgccgt gaccgcgctg ggatcccggt
     7621 cgtcgcgatc acgggcagca acgggaagac caccaccaag gagatgaccg cggccgtgct
     7681 gggcacgtcg cgccgcgtgc acaagaacgt cggaaacctg aacaaccaca tcggcgtgcc
     7741 gctcaccctc acccggctcc gccccgagca cgaggccctg gtcgtcgaga tggggatgag
     7801 cgcgcggggc gagatccgcg atctcgcggc gatggcgcag ccgacgatcg gcgtgctcac
     7861 gaatgcctcg gccgcgcacc tccagcagct cggctcggtg gaagaggtgg cgcgggcgaa
     7921 gagcgagctg gccgaggcgc ttccgacgca gggactcctc gtcctgaacg cggacgaccc
     7981 gcttctctat ccgatgaacc gcgagcggat cgtgcggaag accacctacg ggctcgacaa
     8041 tcccgacgcg gcgctgcgcc ccacgcgcgt cctcgtggac gaggacggaa ccacgcgctt
     8101 caccctcgag gacggcagcg aggggcggct ctccctcctc ggacggcaca acgtgcggaa
     8161 cgcgctcgcg gcgatcgcgg tgggggacga gctgatggtc ccgcgcgacg ccgcgctcgc
     8221 cgccctcgcc gcgctccgcc cggcgaagca tcgcctggag atactccacg ccggcggcgt
     8281 cgccgtgatc gacgacgcct acaacgcgaa ccccgcctcg atgcgcgagg cgctggcgat
     8341 cctgggcggc gtggccgtgg aaggcgagcg gcgggcggtg ctgggcgaca tgctcgagct
     8401 gggtccgggg agcgaggcgc tgcacgagac ggtcggccgc gcggtccccg cgggcgcctg
     8461 gctctacgtc gcggggtcct tcgccgcggc catcgagcgg ggcgccaagg ccgcgggcgt
     8521 cgccgcggcg cggatccgcc gcttcgagga cgtggacgcg atggccgccg cggtggcggc
     8581 ggacgcccgc gcgggcgatc tcgtcctggt gaaaggatcg cgcgggatgc gtctcgagcg
     8641 cgtggtgcag gcgctccagg ccgcgggcgc ccaggccggg gcggggaggg actgacgggt
     8701 gttctaccac ctcctctacc cgctgcacga gcacttcgcg cccttcaacg ttttccgcta
     8761 catcacgttc cgcgccgcgt acgcgctcgc gacggcgctc ttcatcgcgc tcttcctggg
     8821 tccgccggtg atccggcggc tgcgcgcgct gaagatcggc cagaaggtgc gcgacgacgg
     8881 tccgcagagc cacctgccga aggcggggac gcccacgatg ggcggcgtcc tcatggtcat
     8941 ctcgatcgcg gtgaccacgc tgctctgggg caatctctcg aatcggaacg tctggatcgc
     9001 gctcatcgcc acgatctgga tggggacggt cggcttcatc gacgactatc tccgcgtcgt
     9061 gaagaacttc cggaaggggc tcctggggcg ctacaagctg gcggggcaga tcacgctggg
     9121 gatcggggtc ttcgcggcgg tcttcttcta cccggcgcac gggctgctcg acccggccga
     9181 aaccaacatt cccttcttga agcgcacggt cgtggatttc ggatggctct atctcccctt
     9241 cgtgatcctg gtcatcaccg gggcgtcgaa cgcggtgaac ctggccgatg gcttggacgg
     9301 cctcgcggcg gggctcgccg ccttcggcgc ggtcgcgctc ggagggatgt gctacatcac
     9361 gggccacgcc aagttctcgc agtacctcca ggtcccctac atcgccggca ccggggagct
     9421 cgcggtcttc tgcgcggcga tcatcggggc cacgctcggc ttcttgtggt ggaactgcca
     9481 cccggccgac gtcttcatgg gggacacggg atcgctctcc ctgggggccg ccctgggaac
     9541 cgtggcggtg ctcatcaagc gggaattcct gctcgccatc gtctgcgggg tgttcgtgat
     9601 cgaggccctt tcggtcatgg cgcaggtcct ttccttcaag atctggggga tacgagttct
     9661 caagatggct ccgttgcatc atcatttcga gctgagcggt tggaaggaga cgcgcgtcgt
     9721 ggtccgcttc tggatcgccg ccgccctctg cgcgctgttg gccctgagca ccctgaagct
     9781 gcaatgagca cgacgacgca accctggttc gccggatcga aggcgctggt cgtgggactg
     9841 gcgcggagcg gcatcgccgc ggcgcggctg ctccggcgcc acggcgcctc cgtgcgcgcc
     9901 gtggaccggc ggcgcgctga ggagctctcc cccgacgcgg ccgatctcga gtccttgggc
     9961 gtcgagctgc gggccggcgg taccgatccc gcgtccctcg aaggccgcga cctggtcgtc
    10021 gtgagcccgg gggtcccgct cgacctgccg ctcttccgcg aggcggagcg gcgcggcatc
    10081 ccgatcgcgg ccgagatcga gctgggattc gcggtggcca aggcgccgat cgtggcggtg
    10141 acgggcacca acggcaagag cacgacggtg gagcttctcg gcgccttcgg ccgcgccgcg
    10201 ggaaggccga ccgaggtgct cggcaacatc gggaccgccc tctccgagcg cgccgagtcg
    10261 gtgccggaag aggggctgtt ggtcgtcgag gtctccagct tccagctcga gacctgcacg
    10321 cgcttccgcc ccgacgtggg cgtgatcctg aacgtgacgc ccgaccacct ggaccgccac
    10381 ggcacgctgg agcgctacgc cgccgtcaag gcgcggctct tcgcgcacca gaccgaccgc
    10441 gactaccggg tgcagccgct cggcgatccg ctcctcgtct cgctgctcgc cccgatgcgc
    10501 tcgcggccgc tctggttcgg cttcaccgat cccaccgccg acggcgtctg ggaggagggg
    10561 ggcgcgctcc gctcccggtt cgaggggcgc gagggcacca tcgtcaagcg gagcgatgcg
    10621 aagctcgagg ggccccacaa caccgagaac atggcggccg ccgcggcggc ggcgctcgcc
    10681 gtcggaatct ccgagcgggc gatcgcgtcg gcgctccgcc agttccgcgg gctcccgcac
    10741 cggctggcgc tggtcgcgga gatcgacggc gtgcggtacg tgaacgactc caaggcgacg
    10801 aacgtggatt ccatgaagcg cgcgctggag tcgttcggcg acgcgccgat cacgctcatc
    10861 gcgggcggac gcgacaagaa cggcgacttc gcctccatcg ccccgctcgt ggccgagaag
    10921 gtgcggcgcg ccctgtacgt cggggaagcg gcgccaattc tcgagcgcgc ctggccgcac
    10981 ctgtccgccc agcgcgcggc gacgctggag gacgcggtcc gcctcgcgcg cgccgagacg
    11041 ccggcgggcg gggtcgtgct gctgtcgccg ggctgcgcgt cgtacgacat gttccgcaat
    11101 ttcgaggagc gcggcgcccg cttcgaagcg gccgtgcacg cgctgcagcg ggaccccgcg
    11161 cgggcgaagc cgtgaacgtg gccctggatc gcgcgcgccg tcccgacttc aagctgctcg
    11221 ttcccgtcgt ctgcctggtg gcgctgggac tggtgatggt cttctcgtcc agcgcgagcc
    11281 tcgcggcgca ccgctacaac tcggagtact tcttcctgaa gcgccacttc gtccgcgtgc
    11341 tgatcggcgt ggcgcttctg gtcgtagtcg cccgcaccga ctaccacgtg ctgcagcggc
    11401 tcgccgcctg ggcgctgggc ggcacggtgc tcctcctcgg cgcgctgctc gccctgggga
    11461 gcgcggtgcg cggcgccaac cgctggctat cgctcgcgac cgtcacgatc cagcccaccg
    11521 agatcgcgcg cgtcgcctgc gtcgtctatc tggccaagct cctcgaccgg aaaggggagc
    11581 ggatggcctc cttcaaggag ggcgtgctcc cggcctgcat ggtgctcggg ctcatggcct
    11641 tcctcatcct cctccagccg aacctgggga gcacgatcgc gctcctctcg accggcttcg
    11701 tcatgctcca cctctcgggg gcgcggcggg cgcatctggc gctcctcgcc ctcgccggcg
    11761 tgctcgtggc cgtgctcgcc gtctcccaca acggctacat gatggagcgg gtccagggat
    11821 ggctcgccca gtgggggcac ggccgctcgg actcgctcgg caagaactgg caggtgtcgc
    11881 agtcgatcct ggccctgggc tccgggggcg tcctcggcgc cgggccgggt catggtctgc
    11941 agaaggtgtt cttcctcccc gacccgcaca ccgacttcat cttcgcggtc atcggggagg
    12001 agctgggtct tctgggaacg gcgggcgtgc tcgccgcctt ctcggttctg ttcctgcgtg
    12061 gactgaaggt cgcggccgga gcgccggacc ggttcggcta tctgctggcc gccggcctca
    12121 cggtcaacct gtcggtctac gtcgccatga acatcgcagt ggtgaccggg gtgattccca
    12181 ccaccggcct gccgctgccg ttcgtcagct atgggggctc ggccctcgtc gtcaacttgg
    12241 gggtaatcgg tgttctcctc aacatcgccc atcaaatgga gacagggtat cggacgtccg
    12301 cggtcgtccg gccgcccgga gtcggacgcc ggcgcgtccg atagggtcgc accgctccgc
    12361 ctgctgatcg cggccggcgg aaccgggggg cacgtctacc ccgggatcgc ggtcgccgag
    12421 gaatggcggc ggcggcatcc cgattcggaa gtggtgttcg tcggcacggc gcgcggcgtc
    12481 gagagcacgg cggtgccgca ggcggggttc gcgctccgca ccatcgccgc gcgcgggatc
    12541 ccgcggcggc tggggttcgg cgtgatacgg gccgtggccg cgatgatcca gagcttcgtg
    12601 caggcgcagt cgctcctgcg cgatctgaag ccgcaggtcg tggtggtgac gggaggctac
    12661 gtgagcggac cggtcgggct ggcggcgaag cttcgcggca tcccggtcgt ggtgcaggag
    12721 cagaactcgg tgccgggggc gacgaacctc tggctcaacc tgatcgccga cgaggtgcac
    12781 atctcgttcg tggaatcgcg gaactacttc cggaggcgga acaacctgaa agtcaccggc
    12841 aatcccatcc gccgctcgct gctccgccag gaccggacca gcgcctacga gttcctgcgc
    12901 ctcgacccgc atcggagcac gctgctcgtg ttcggcggga gccgcggcgc caccagcatc
    12961 aaccgggcgt tccaggacgc gctgccgcgc ctgacgcggc tccagaacat ccaggtgctg
    13021 tggcagacgg ggaaggacga ggcggcggcg atccgcgacc gcgtgaagtc gctctcgatc
    13081 ccggtgcacg tgctgcccta tctcgaccag atggagaagg catacgccgt cgccgacctc
    13141 gcggtgtgcc gcgccggcgc gatgacgatc gccgagctga cggcgtgcgg ggtgccctcc
    13201 atcctgatcc cctatccgca cgccacccac gatcaccaga cggtgaacgc gcgcggcctg
    13261 atggagcggg gcgccgccga ggtgatccgg gacgacgagc tggaggcgga ggacctggcg
    13321 aagcggatcg tccagctctt ccaggacgag ccgcggctcc gccgcatggc gcggaacgcc
    13381 cgggccttct cgcgcacgaa cgcggccgag cgactggtga cctcgatcga gtcgctcgcg
    13441 ggggcggtcc ccgcggagcg gatgggttag acgtgtacgg acgcatccgg aggatccacc
    13501 tggtgggtat cggcgggagc gggatgagcg ggatcgccga ggtgctgctc aacctgggtt
    13561 acgaggtgac cggctccgac ccgaaagagg gggaggcggt cgcgcggctt cgcgacatcg
    13621 gcgcgcggat cgcgtccacg cacgacgcca tgcacgtggt gggcgcggac gtcgtggtgg
    13681 tctcgagcgc cattcccgaa accaatcccg agatccgccg cgcccgcgcg ctcggcatcc
    13741 cggtgatccc gcgcgcggag atgctcgcgg agctgatgcg gatcaagacg tccgtcgccg
    13801 tcgcgggagc gcacggcaag acgacgacga cctccatggt gggggagatc ctcgccaacg
    13861 gcggcctcga cccgacggtg atcgtgggcg gccgcctcaa gaccgtgggc gcgcacgcgc
    13921 gcctggggca gggccccttc ctggtggccg aggccgacga gagcgacggg tccttcctgc
    13981 tcctcgcgcc gacgatcgcc gtgatcacga acatcgacgc cgagcacctg gactatcaca
    14041 agtcgctcga gggggtgcag ctcgcgttca cggcgttcgc gaaccggatc cccttctacg
    14101 gcacgatcat cctgcccggc gacgacccga acgccgcgcc gatccgcgcg cgcgccaagc
    14161 gtcgcgcgct gacctacggc ttcacgccgg gactcgactt cgaggggaag aacctggacg
    14221 tgggacccgc cggcgtgcgg ttcgatctgc tcgtgcgcgg cgagtcggag ggctcgatcg
    14281 agctctcggt cgccggcgcg cacaacgcgc ggaacgcgct cgccgcgctc gccgtcggct
    14341 gggagctggg cgttccgatc gcggcgatcc gcggcgccct ccggagcttc gccggtgtcg
    14401 ggcggcggct ggagcggcgc ggggatctcc tcggggcgct ctggatcgac gactacggcc
    14461 atcaccccac ggagatcgag gccgcgatcg ccgcgctgcg cgccacctac gagcggcgga
    14521 tcgtcgcggt cttccagccg caccgctaca cgcgcacgca cgcgctggcc gaccgcttcg
    14581 cgacggcttt ccgcggcgcg gccgagctgg tgctcctccc gatctatccc gcgggcgagg
    14641 cgccgatccc gggcgtcacc gcggagtcgc tggccgagcg gattcgcgga ggcagcggcg
    14701 tgacggtgcg ctacgccgcc gattacgacg acgcggcgcg cgtcgcgcgc gacctggtgc
    14761 gcgacgggga ccttttcctc acgatcggcg cgggcgacgt cttcaaggtc ggcgagatca
    14821 tcctggcgga gggggaaaac gcccccgccg cggcggggcg cgcgtgagag cgggctaccg
    14881 ggcgctccct tcctggaaga agccggcggt gaagcggcgc cgccggagaa tcgtgatgct
    14941 cctcgctctg agcgcgctcg ccaccctgac ggcgccgctc gccgtgaagc ccgcggcgcg
    15001 cgccctggga tcgctccagc cgttccaggt gcgctcgatc gaggtgtccg gacttctcta
    15061 cctggcgccc gaggagatcc gctcgagcgt cgcccaggcg ggggcgcgcg tcggggacaa
    15121 cctcctcctc gtcagcccgg cgcgcgtgga ggccgggatc gagaagaacg cgcgcgtgga
    15181 gcgcgcgcag gtcctgcgcg tacccggaaa gctgatcgtg cgcgtgaccg agcgacggac
    15241 cttcgtgctg gtgagcgcgg ggtcgctcct cgaggtggac gccgacggca ccgtcctctc
    15301 gccgctgaag cgcgggctgg tcgccgaccg cccgatcgtc acggggctca agatccgtcc
    15361 cgccgccccc ggctccaagc tctcgggcga acggatgcag gacctgcttc gcctggtgcg
    15421 gctcctcgag tcgcccgagg tggggctggt ctccgagatt tccgagatcg cgtccgacgc
    15481 gccggcgcgg gccgtgctcc gcacgtcgcg cgaccagata ccgatcgtcg tcgaccccga
    15541 gcgggtgacg ctggccgcga tgcgcgcggt cgccgccacg ctccgggacg tcagggaacg
    15601 agaccgccgc gtcctcgtca tggatgcgcg gtaccgaggg caggtcgtgg tgcgctgcgg
    15661 cccggacagc tcgacgggcg cgcgcacggg cgtggatccg cgggccaaag tctagccagg
    15721 ggcgccggga ggggaggaat ggaagccaag atctacgcgg ggctggacat cgggacgacc
    15781 aagatcaccg ccatcgtcgc cgagccggac gagaacggcg agtcgctgcg catcatcggc
    15841 gtgggcaccg ccccgtccga cggcttgaag cgcggcgtcg tcgtgaacct cgagaagacg
    15901 gtgcgctcga tccagcacgc gctggaagaa gcggagcgcg tctcgggacg atcgatcaag
    15961 agcgtgtggg ccggcatcgc cggggaccac atccgcggac tcaccagccg gggcgtcatc
    16021 gcggtctccc gcaaggacgc ggagatccgc cagaacgacg tggcgcgcgt catcgaggcc
    16081 gccaaggcgg tcgcgattcc ggccgaccgg gagatcctgc acgtcctgcc gcaagaattc
    16141 atcgtggacg accaggaggg aatccgcgat ccggtcggga tgagcggcgt gcggctcgag
    16201 gccgaggcgc acatcatcac gggcgccgcc gcggcgtgcc ggaacgtgat ccgcgccgcc
    16261 gagcgcgccg gcgtgcacgt cgaggagctg gtgctggagc cgctcgcctc ggcgaacgcg
    16321 gtgctcgctc ccgacgagaa ggacctgggc gtggcgctcc tcgacatcgg cggagggacc
    16381 accgacgtgg cggtcttttt cgagggggcg atccgccaca cggcggtgat cggcctgggc
    16441 ggatcgaacg tgacgaacga cctggccatc ggccttcgca cgccggtgga gcgggcggaa
    16501 atcttgaagc tggggagcgg gtgcgcgctg acctccatgg tgcgtcccga cgagacggtg
    16561 aaggtgccga gcgtgggggg gcggcccgac cgggacatct cccgccacat gctcgcgatg
    16621 atgatcgagc cgcgggtgga ggagattttc gcgatggcca atcgcgagat ccggaagagc
    16681 cacgtcgccg acctcctggg ggccggcgtg gtgctgacgg gaggcgcctc gtcgctcccc
    16741 ggcatgcccg agctcgcgga gcaggtcttc gacctgccgg tgcggcgggg cattccgacc
    16801 ggcgtgacgg gcatggtcga agcggtttcg gatccccggt tcgcgacggg tgtggggctg
    16861 gcgcagcacg cgttctggaa cgacgcgagc ccgcacgtcg ccggagaggg attgttcggg
    16921 cggatctcga tcgggatccg ccgctggatc gaggagctgg tctagacgtg agggttcggg
    16981 ggagctgggc tttggggatg accggaggca ccggcggaca cgcaacaggg aggggatgac
    17041 gatgttcgag ttggtcgatg agggtgggga gcggtccgga gcctcgatca aggtgatcgg
    17101 cgtcgggggc gcgggcggga acgcggtcaa tcgcatgatg gaatccggcc tcacgggcgt
    17161 ggagttcatc gtggcgaaca cggatgcgca ggtgctggtt cagtcgcgct gcccgcggaa
    17221 gatccagctg ggcaccgcga tcacgaaggg cctcggctcg ggagcgaacc ccgcggtcgg
    17281 gcgtcagtcg gcggaagcgg acgaggaagt gatccaggag gcgctctccg gatcggacat
    17341 ggtgttcgtg acggccggca tgggcggcgg cacgggcacc ggcgcggcac cggtggtggc
    17401 gcggatcgcg cgctcgctgg gcgctctgac ggtgggcgtc gtcaccaagc ccttcgagtt
    17461 cgaggggcgg cggcgcgcgc agtcggcgga cgagggcctg cgggagctgc gggagaacgt
    17521 ggacacgctg atcgtgatcc cgaaccagcg gctgctcgcg atcgtggaga agaacacgcc
    17581 gctccgcgac gcgttcaaga cggcggacca ggtgctgcac cacgccacga aggggatctc
    17641 ggacctgatc acggtcccgg gtctggtgaa cctggacttc gccgacgtga agacggtcat
    17701 ggccgagcgg gggaacgcgc tcatgggcgc gggctacgcg accggcccga cgcgcgccta
    17761 cgaagcggcg cagtcggcgg tgtcgagccc gctgctcgac gacatctcga tctccggcgc
    17821 cgaggcgctg ctggtgaacg tcaccggcgg cgagtcgatg acgctgcacg agatcagcga
    17881 ggcggtgtcg gtggtcgtgg acgcggcggg tcacgaagcg aacgtgatct tcggcgccgt
    17941 gatcgacgac cgcatgggcg acggcctctc gatcacggtc atcgcgacgg gcttcgggcg
    18001 ggccgaggcg aagatgcgcc cggcaacgat cccgatgggg gcgcgcacgc cggtcgcgcg
    18061 gccgcaggcc gcgccggtcg ccgcctcggc ggcggcccca gctcagattt tcgagctgga
    18121 gcagcgtccg gcgatgaagc aggtgcgcgc cgcgatggag gagctggaag aagaggaaaa
    18181 tgacgcggtg gagacgccgg agccggcgcc cgccgcatcg aacgggcggc cctcgttccg
    18241 gattccgccg ggcaccgccc gtcggccgtt cgggggacga gcgatcaccc gggacaacat
    18301 ggacgtcccg gcgttcatcc ggaagcaaat ggactagggc cttaggggga gggtgtgatc
    18361 ggcgcagggg cgccgagaga gtgaggtggc ggcgccgctg cgacggcgct aaggtgctgt
    18421 gaaggggagg agccgggtcg gcc
//
LOCUS       DASSLZ010000276         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1242255, whole genome shotgun sequence.
ACCESSION   DASSLZ010000276 DASSLZ010000000
VERSION     DASSLZ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242255"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..931)
                     /locus_tag="VFM00_10515"
     CDS             complement(20..931)
                     /locus_tag="VFM00_10515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HET7498723.1"
                     /translation="MNPEGLAGPALAVLFAPLFLGLIHRTKAFFAGRSGPPLLQLYHD
                     LGKLLRKGAVLSRTTTWVFGLGPVAGVAAVLAALTLLPQAGLPAAFAFPGDFVLLLYL
                     LGTARFLTMAAALDTGSSFEGMGASREATFSALAEPVLLLCLAAAARVSGHTSLNGIV
                     GGLTEADWQRGAPVLVLVGAAVLAVLLAENARMPVDDPGTHLELTMIHEVMVLDHGGP
                     DLAMIHWGAALKLWAMGALLVGLLLPVRTGSLAVDGGTFLLAMALLAVVIGVVESTIA
                     RLRMNRVPQFLVGAGAFAVVAIALILR"
     gene            complement(928..>2412)
                     /locus_tag="VFM00_10520"
     CDS             complement(928..>2412)
                     /locus_tag="VFM00_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET7498724.1"
                     /translation="LGLAGSAPTLDFGVPPAGSASAPAAAPAPAGTFLGSSALFLLAV
                     LGFGTKAGFMPLHMWLPEAHPAAPAHVSAVMSGVMIKMGIYGLLRMLPYLGPPPAWWG
                     WLLIAIGLVSGILGVLYALAQHELKRLLAYHSIENIGIIACGMGLGLLGTAQGTPFLA
                     ALGFAGALLHVLNHAVFKGLLFLGAGSVIRGAGTGEIEHLGGLLRRMPWTGAAFLTGA
                     LAISGLPPLNGFVSEFLIYLGSFQAAAASPGPVALAGMVLIAGLALIGGLAAACFVKA
                     FGIAFLGEPRSPHAAEARESGALLRAPMAILAALCLAIGLLAPRFLALLLPALPYGAA
                     FPASALGAGFGTATAIVMRVTTVGGLFLLLVLAGVVLRRGLLSRRVVRRSGTWDCGYA
                     LPTPRMQYTASSFAQPILEVFTPLLGTRVSSLKPMGLFPARASHATETPDSFRERVVA
                     PIAAEIARRVAPFRRIQEGRVQVYVLYIALTLVAVLVYQFLVNP"
BASE COUNT          442 a          841 c          887 g          242 t
ORIGIN      
        1 caggaggagg cgcatggccc tatcgcagga tcagcgcgat cgcgaccacc gcgaacgccc
       61 cggctccgac caggaactgc ggcacgcggt tcatgcgcag gcgggcgatc gtcgactcga
      121 cgacgccgat caccaccgcg aggagcgcca tcgcgaggag gaaggtgccc ccgtccacgg
      181 ccaggctccc ggtgcgcacc ggaaggagca acccgaccag gagcgccccc atcgcccaga
      241 gcttcaaggc cgcgccccag tggatcatcg cgagatccgg cccgccatgg tccagcacca
      301 tcacctcatg gatcatcgtc agctccagat gcgtgcccgg atcgtccacc ggcatgcgcg
      361 cgttctccgc gagcaggacg gcgaggaccg ccgcgccgac gaggacgagg accggggcgc
      421 cgcgctgcca gtccgcctcg gtgagaccgc cgacgatccc gttcaacgaa gtgtgccccg
      481 agacgcgcgc ggccgcggcc aggcagagca ggaggaccgg ttcggcgagg gccgagaagg
      541 tcgcctcgcg gctcgcgccc atcccctcga agctcgaccc ggtgtccagc gcggcagcca
      601 tcgtcaggaa gcgcgccgtg cccaggaggt agaggagcag gacgaaatcg ccggggaagg
      661 cgaacgccgc cggaaggccg gcctgcggca gcagcgtgag cgcggcgagc accgcggcca
      721 cgcccgcgac cgggccaagt ccgaacaccc aggtcgtggt ccggctcagc acggcaccct
      781 tccggagcag cttcccgagg tcgtggtaga gctggaggag cggagggccg ctccgtcccg
      841 cgaagaaggc cttggtgcga tggatcaggc ccaggaaaag aggcgcgaac agaacggcga
      901 gcgcgggtcc cgcgagaccc tccggattca tgggttcacc aggaactggt agacgagaac
      961 ggcgacgagg gtgagggcga tgtagagcac gtacacctgc acccttccct cctggatcct
     1021 ccggaacgga gcgacgcggc gcgcgatctc ggccgcgatc ggagcgacca cccgctcgcg
     1081 gaacgagtcc ggcgtctcgg tggcgtgcga cgccctggcc ggaaagagtc ccatcggctt
     1141 cagcgaggag acccgcgtgc ccaggagcgg agtgaagacc tcgaggatcg gctgggcgaa
     1201 ggaggacgcc gtgtattgca tgcgcggcgt cggcagggcg tagccgcagt cccacgttcc
     1261 gctccgccgg accacgcggc gcgagagcaa cccccgccgc agcacgaccc ccgcgagaac
     1321 gagcaggagg aagaggccgc ccaccgtcgt gacgcgcatc acgatcgccg tcgccgtgcc
     1381 gaagccggca ccgagcgcgg aggcgggaaa cgccgcaccg tacggaagcg ccggcaggag
     1441 gagggcaagg aaccgcgggg ccaggagccc gatcgccaga cacagcgccg ccaggatcgc
     1501 catcggcgcg cggaggagag cgcccgactc gcgcgcctcg gccgcgtggg ggctcctggg
     1561 ctcgccgagg aacgcgatgc cgaacgcttt cacgaagcac gccgcggcca gcccgccgat
     1621 cagcgcgagc cccgcgatca gcaccatccc cgccagcgcg accggccccg gcgaggcggc
     1681 cgcggcctgg aacgatccca ggtagatcag gaattcgctg acgaagccgt tgagcggcgg
     1741 gagcccggag atcgcgagcg cgccggtcag gaaggccgcc ccggtccagg gcatgcggcg
     1801 gaggagtcct cccagatgct cgatctcacc ggtcccggcc ccgcggatga ccgaacccgc
     1861 tcccaggaag aggagcccct tgaacacggc gtgattgagc acgtggagga gcgcgccggc
     1921 gaagccgagc gccgcgagga acggcgtccc ctgcgccgtg ccgaggagcc caagtcccat
     1981 cccgcacgcg atgatgccga tgttctcgat gctgtgatag gcgaggagcc gcttcagctc
     2041 gtgctgcgcg agcgcgtaga ggacgccgag gattcccgag acgaggccga tggcgatcag
     2101 gagccacccc caccaggccg gcggcggccc caggtagggc agcatgcgga ggaggccgta
     2161 gatccccatc ttgatcatca cgcccgacat cacggcggaa acgtgcgctg gcgccgcggg
     2221 gtgcgcttcg ggaagccaca tgtggagcgg catgaacccg gccttggtgc cgaagccgag
     2281 gacggcgagc aggaagaggg cggaggaacc gaggaaggtt cccgccgggg ccggggccgc
     2341 ggccggggcg gacgccgatc cggcgggcgg caccccgaag tccagcgtgg gcgccgatcc
     2401 ggcgaggccg ag
//
LOCUS       DASSLZ010000277         6820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1621727, whole genome shotgun sequence.
ACCESSION   DASSLZ010000277 DASSLZ010000000
VERSION     DASSLZ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621727"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /gene="atpB"
                     /locus_tag="VFM00_10525"
     CDS             <1..1213
                     /gene="atpB"
                     /locus_tag="VFM00_10525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET7498725.1"
                     /translation="WSRLEIWPLVAGLSLPLAAIFLKAVMLALTPGRSPFGAKRAAGS
                     TLASILLAGALLTAGLAAVATVAPSRALAQRTVTEEETTPAAPPAATAPEGSAAHTVS
                     PPNAPPHDVPAEGGEHGGGGEQHLPNWLTILNDWMPKNPVVHWMHENEYLVFAWIAGL
                     VFLGIVVLFMKRDAMVPGPVQNAIEWVVESMEDVVGGMLGKHTHRYFPFIMAIFFYIL
                     TMNMIGIIPGFKSSTSTLDVTLALALITFLFVQFSGIRNLGILGYLDHLAGSPRDVVG
                     FAMLPVMIPVHILGELVKPVSLSCRLFGNIFGEDTLIVVFVGVAALCLKASLTALAVP
                     PMLGITALFMLLQTLTSIVQALIFSLLTTVYLYMMLPHEAHDHGHAEHAPADVPHVGG
                     SFPTDTKEVKS"
     gene            1210..1443
                     /gene="atpE"
                     /locus_tag="VFM00_10530"
     CDS             1210..1443
                     /gene="atpE"
                     /locus_tag="VFM00_10530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET7498726.1"
                     /translation="MNFAATLGLALPIGIGLAAIGSGIGLGFMGRGAMEAMGRQPEAL
                     PRIQVAMIIGFGFIEALTIYAFVTMFFLQGKIQ"
     gene            1508..1999
                     /gene="atpF"
                     /locus_tag="VFM00_10535"
     CDS             1508..1999
                     /gene="atpF"
                     /locus_tag="VFM00_10535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET7498727.1"
                     /translation="MDQFVHLNQLLAHALSFIVFFFLVRAAFAAIIYPPMRERKERIV
                     AELAGIEKGKAAVEDLKAQYEAHLKRIEAESRDRIQQAVAEGQRVGAEIREIARKEAQ
                     ETITRAREEITLERDKARVELKNEVVELAIEIAGKVVREELSPARHQKLLDEFLAGVG
                     EAR"
     gene            1996..2547
                     /gene="atpH"
                     /locus_tag="VFM00_10540"
     CDS             1996..2547
                     /gene="atpH"
                     /locus_tag="VFM00_10540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET7498728.1"
                     /translation="MISVTLARRYARALLHLAQKHASLNQVEEELREIAGLARRDVRV
                     RRFFETPGIARTEKIAFLEKSWKPRLSRPVYGLLVVLLRRRRLDHLIVIAHEFQKLAE
                     SAKGLSRLLVRTAVPLSEAQAGTLTRALAARTGRTIVLTREVDPSLIGGAVVSLDHHV
                     IDGTLATELWRIRRRLLATRVHG"
     gene            2558..4081
                     /gene="atpA"
                     /locus_tag="VFM00_10545"
     CDS             2558..4081
                     /gene="atpA"
                     /locus_tag="VFM00_10545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET7498729.1"
                     /translation="MAFKPEEVSAVLEQEIERYESRLEMESVGTVLQVGDGIARVWGL
                     DDAMSGEMVLFPGDVNGIVLNLEEDNVGVALFGSEAAIKQGDTVRRTGKLASIPVGEA
                     MVGRVVNAIGIPIDGKGPIATTETRSLEGHAPGVVDRQPVNQPVQTGLKAIDSMIPIG
                     RGQRELIIGDRQTGKTAVAIDTILNQKGGDLFCIYVAVGQKDFTVAKVVKILEDHGAM
                     EYTTVVAAGASETAPLQYIAPYTGCAIGEYFRDKGKHALVIYDDLSKHAAAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDQLGAGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLESDLFFAGIRPAINVGISVSRVGGKAQTKAMKKIAGRLRLDLAQYREL
                     AAFAQFGSDLDKATQAQLTRGERMVELLKQGQYQPLPVERQVAIVYAGTNGYLDDLPV
                     TSIRDFEIGLYEFLDKQYADVLHDLKVKYELTDAIEARLRKAIEAFKAEFATRKVKP"
     gene            4101..4952
                     /gene="atpG"
                     /locus_tag="VFM00_10550"
     CDS             4101..4952
                     /gene="atpG"
                     /locus_tag="VFM00_10550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET7498730.1"
                     /translation="MATLREIRTRIRSVRNTAQITKTMEMVSAAKLRRAQTAVESARP
                     YATLLGEVLQNLAQASGDVLHPAFVRREVKARVIVLITSDRGLAGAFNANLIRTVEAR
                     VREAQRPTRFVLLGKKGYAYFRRRNADILAFRDDMGGVADWKYAEAVSRDLLERFLSG
                     EIDEVDLVTTHFKSALSREIVVTPLLPLGPERAPAGPTRDYIFEPGPEEILARIVPYF
                     LAMRLYTALAESAASEHGARMIAMGSATKNANEMIQNLTLHMNRTRQATITREIVEIV
                     GGAEALK"
     gene            4972..6423
                     /gene="atpD"
                     /locus_tag="VFM00_10555"
     CDS             4972..6423
                     /gene="atpD"
                     /locus_tag="VFM00_10555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET7498731.1"
                     /translation="MANANGKNGNAGGENIGQIIQVIGPTVDVRFEPERLPKILNALT
                     IQDADRGIDLTVEVALHIGDEVVRCVAMSSTDGVTRGMAVRDTGGPITVPVGRQTLGR
                     VFNLLGATIDQGGPVKEPEKRLPIHRAPPSFEEQETVTRIFETGIKVVDLLAPYAKGG
                     KIGLFGGAGLGKTVILQELIRNIATEHGGFSVFSGVGERTREGNDLWLEMKESGVIDK
                     TVMVFGQMNEPPGARLRVALTGVTMAEYFRDQENQDVLFFVDNIFRFTQAGSEVSALL
                     GRMPSAVGYQPTLSTEMGALQERITSTKRGSITSVQAIYVPADDLTDPAPATTFTHLD
                     ATTVLSRRISELGIYPAVDPLDSTSRILDPNIVGEEHYNLARAVQKILQRYKELQDII
                     AILGIDELSDDDKLIVARARKIQRFLSQPFFVAEPFTGTPGKYVKLEDTIRSFQRIVA
                     GDCDHIPEQAFYMMGTIDDVFAKAEQMGVTAGA"
     gene            6439..6705
                     /gene="atpC"
                     /locus_tag="VFM00_10560"
     CDS             6439..6705
                     /gene="atpC"
                     /locus_tag="VFM00_10560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET7498732.1"
                     /translation="MAEFTFRLVTPQRIVFEGPVVSIQAPGSEGYLGVLAHHAPLITM
                     LKDGRLEMRDPSGRVTAWHVTGGFLEVSNNRASALADSAEPITP"
BASE COUNT         1137 a         2354 c         2280 g         1049 t
ORIGIN      
        1 atggagccgg ctcgaaatct ggcctctcgt ggccggtctc tcgctcccgc tcgccgcgat
       61 cttcctgaag gcggtcatgc tcgcgctcac gccgggtcgc tcgccgttcg gcgcgaagag
      121 agccgccgga tcgacgctgg cctcgatcct cctggccggg gcgctcctca cggccgggct
      181 cgcggcggtc gcgacggtgg ccccctcgcg cgcgctggcc cagcggaccg tcacggagga
      241 agagaccacg cccgcggcgc cgccggcggc cacggcgccc gagggctccg ccgcgcatac
      301 ggtgtcgccg cccaacgcgc cgccgcacga cgttcccgcc gaaggcgggg agcacggcgg
      361 cgggggcgag cagcacctgc ccaactggct caccatcctg aacgactgga tgcccaagaa
      421 cccggtcgtc cactggatgc acgagaacga atacctggtc ttcgcgtgga tcgccgggct
      481 cgtcttcctc gggatcgtcg tcttgttcat gaagcgcgac gccatggtgc cgggccccgt
      541 ccagaacgcc atcgagtggg tggtggagag catggaggac gtcgtggggg ggatgctcgg
      601 gaagcacacg caccgctact tccccttcat catggcgatc ttcttctaca tcctgacgat
      661 gaacatgatc ggcatcatcc cgggcttcaa gtcctcgacc tcgacgctcg acgtgacgct
      721 ggcgctcgcg ctcatcacgt tcctcttcgt gcagttctcg ggcatccgga acctgggcat
      781 cctgggctac ctggaccatc tggccgggtc gccgcgggac gtcgtgggct tcgccatgct
      841 cccggtcatg atcccggtgc acatcctggg cgaattggtg aagccggtca gcctctcctg
      901 ccgactcttc ggcaacatct tcggcgagga cacgctgatc gtggtcttcg tcggcgtggc
      961 ggcgctgtgc ctcaaggcgt cgctgaccgc gctcgcggtg ccgcccatgc tgggcatcac
     1021 ggcgctcttc atgctgctcc agacgctgac gagcatcgtg caggcgctga tcttctcgct
     1081 tttgaccacg gtgtatctct acatgatgct gccccacgaa gcgcacgacc acggccacgc
     1141 ggagcacgcg ccggcggacg tgccgcacgt gggcggctcc ttccccacgg acacgaagga
     1201 ggtgaaatca tgaatttcgc tgcgacactc ggcctggcgc tcccgatcgg gatcggcctc
     1261 gccgccatcg gctcgggcat cggcctcggc ttcatgggcc gcggcgcgat ggaggccatg
     1321 ggccgccagc cggaagcgct tccgcgcatc caggtcgcca tgatcatcgg cttcggcttc
     1381 atcgaggcgc tcacgattta cgcgttcgtc acgatgttct tccttcaggg gaagatccag
     1441 taaccggccg gaagcggcgc cggccggtgg tttcggtcgg ccgcccttcc cgagcccgag
     1501 aaggcgcatg gatcaattcg ttcatctgaa tcagttgctc gcgcacgcac tcagcttcat
     1561 cgtcttcttc ttcctcgtgc gcgccgcgtt tgcggcgatc atctatccgc cgatgcgcga
     1621 gcggaaagag cgcatcgtgg ccgagctggc cggaatcgag aaggggaagg ccgcggtcga
     1681 ggatttgaag gcgcagtacg aggcgcatct caagcgcatc gaggccgaga gccgcgaccg
     1741 catccagcag gccgtggccg agggacagcg cgtgggcgcc gagatccggg agatcgcccg
     1801 gaaggaggcg caggagacga tcacgcgcgc gcgcgaggag atcaccctcg agcgcgacaa
     1861 ggcgcgggtc gagctcaaga acgaggtcgt cgagctcgcg atcgagatcg cggggaaggt
     1921 cgtgcgggaa gagctctcgc ccgcgcgcca ccagaagctg ctcgacgagt tcctggccgg
     1981 agtgggcgag gcgcggtgat ctcggtcacg ctggcgcgcc gctacgcgcg cgccctcctg
     2041 cacctggcgc agaagcacgc ctccctgaac caggtcgaag aggaactccg cgagatcgcg
     2101 ggactcgcgc ggcgcgacgt tcgcgtccgc cgcttcttcg agaccccggg catcgcgcgc
     2161 acggagaaga tcgccttcct ggagaaatcc tggaagccgc gtctgtcgcg cccggtgtac
     2221 ggactcctgg tcgtcctgct ccgccgccgc cggctggatc acctgatcgt gatcgcccac
     2281 gaattccaga agctggccga gtcggcgaag gggctcagcc ggctcctggt gcgcaccgcc
     2341 gtcccgctct ccgaggcgca ggccggcacg ctcacgcgcg cgctggccgc ccgcaccgga
     2401 cgcaccatcg tgctcacgcg cgaggtggac ccctccctca tcgggggtgc ggtcgtctcg
     2461 ctggatcatc atgtcatcga cgggaccctc gcgaccgagc tctggcggat ccgccgccgg
     2521 ctcctcgcca cccgggttca cggctagagg ttcgtacatg gcattcaagc ctgaagaagt
     2581 cagcgcggtg ctcgagcagg agatcgaacg gtacgaatcc cggctcgaga tggagagcgt
     2641 gggaacggtg ctccaggtgg gcgacgggat cgcccgcgtc tgggggctgg acgacgccat
     2701 gtccggagag atggtgctgt tcccgggcga cgtgaacggc atcgtcctga acctggaaga
     2761 ggacaacgtc ggcgtcgcgc tcttcggctc cgaagcggcg atcaagcagg gcgacacggt
     2821 gcgccgcacc ggcaagctcg cgtcgattcc cgtcggcgag gcgatggtcg gccgcgtcgt
     2881 gaacgcgatc ggcattccga tcgacggcaa gggcccgatc gcgaccacgg agacgcgctc
     2941 gctcgaaggg cacgcgccgg gcgtcgtcga ccgccagccg gtgaatcagc cggtgcagac
     3001 cggcctcaag gcgatcgact cgatgatccc gatcggccgc ggccagcgcg agctgatcat
     3061 cggcgaccgc cagaccggca agaccgccgt cgcgatcgac acgatcctga accagaaggg
     3121 gggcgacctc ttctgcatct acgtcgccgt cggccagaag gacttcaccg tcgccaaggt
     3181 ggtgaagatc ctcgaggacc acggcgccat ggagtacacc accgtcgtgg ccgcgggcgc
     3241 cagcgagacg gcgccgctcc agtacatcgc gccctacacc ggctgcgcca tcggcgagta
     3301 cttccgcgac aaggggaagc acgcgctggt gatctacgac gatctctcca agcacgccgc
     3361 ggcgtatcgc cagctctcgc tgctcctgcg ccggccgccg ggccgcgaag cctatcccgg
     3421 ggacgtcttc tatctccatt cgcggctcct cgagcgcgcg gccaagctga acgaccagct
     3481 gggcgcgggc agcctcacgg cgctcccgat catcgagact caggcgggcg acgtctcggc
     3541 ctacatcccg accaacgtca tctcgatcac cgacgggcag atcttcctcg agagcgacct
     3601 cttcttcgcg ggcatccggc cggcgatcaa cgtcggcatc tcggtgtcgc gcgtcggtgg
     3661 caaggcgcag accaaggcga tgaagaagat cgccgggcgc ctgcggctgg acctggcgca
     3721 gtaccgcgag ctcgcggcct tcgcgcagtt cggctccgac ctggacaagg cgacgcaggc
     3781 ccagctgacg cgcggcgagc gcatggtcga gctgttgaag caggggcagt atcagcccct
     3841 cccggtggag cggcaggtcg cgatcgtcta cgccggcacg aacggctacc tggacgatct
     3901 tccggtgacg tcgatccggg acttcgagat cggcctgtac gagttcctgg acaagcagta
     3961 cgccgacgtc ctgcacgacc tgaaggtgaa gtacgagctg accgacgcca tcgaggcgcg
     4021 cttgaggaag gcgatcgagg ccttcaaagc cgagttcgcg acgcgcaagg tcaagccttg
     4081 aacgagagcc gggcccggta atggcgaccc ttcgcgaaat ccggacgcgg atccgctccg
     4141 tgcggaacac cgcgcagatc acgaagacca tggagatggt ctccgcggcc aagcttcggc
     4201 gcgcgcagac cgccgtggag tcggcgcgtc cctacgcgac cctcctcggc gaggtgctcc
     4261 agaacctggc ccaggcctcg ggagacgttc tccacccggc cttcgtccgg cgcgaggtga
     4321 aagcgcgcgt gatcgtcctc atcacgtcgg accgcggcct ggccggtgcc ttcaacgcga
     4381 acctgatccg caccgtggag gcgcgggtcc gcgaggccca gcggccgacg cggttcgtgc
     4441 tcctgggcaa gaaggggtac gcctacttcc ggcgccggaa cgccgacatc ctcgcgttcc
     4501 gggacgacat gggcggcgtg gccgactgga agtacgcgga ggcggtgagc cgcgatcttc
     4561 tcgagcggtt tctctccgga gagatcgacg aggtcgatct ggtgaccacg catttcaaga
     4621 gcgcgctgtc gcgcgagatc gtggtcacgc cgctcctgcc cctcggaccg gagcgcgcgc
     4681 cggcgggacc gacgcgcgac tatatcttcg agcccggccc ggaggagatc ctggcccgga
     4741 tcgtcccgta cttcctcgcg atgcgcctct acaccgcgct ggccgagtcg gccgcttccg
     4801 agcacggcgc gcggatgatc gcgatgggct cggccaccaa gaacgcgaac gaaatgatcc
     4861 agaatctcac gctgcacatg aaccggaccc ggcaggccac gatcacccgc gagatcgtcg
     4921 agatcgtcgg cggggccgag gcgctgaagt aggaaggacg aaggaaggac gatggcgaac
     4981 gcgaacggca agaacggaaa cgcaggcggc gagaacatcg ggcagatcat ccaggtcatc
     5041 ggtcccacgg tggacgtgcg gttcgagccc gagcggctgc cgaagatcct gaacgcgctc
     5101 acgatccagg acgccgaccg agggatcgac ctcaccgtcg aggtcgccct ccacatcggc
     5161 gacgaggtcg tgcgctgcgt ggcgatgtcc tccaccgacg gcgtcacgcg cggcatggcc
     5221 gtgcgcgaca cgggcggccc gatcacggtc cccgtcggcc gccagaccct gggccgcgtc
     5281 ttcaacctgc tcggcgccac catcgaccag ggaggccccg tgaaggagcc ggagaagcgg
     5341 ctcccgatcc accgcgctcc gccttccttc gaagagcagg agaccgtgac ccggatcttc
     5401 gagaccggga tcaaggtcgt ggacctgctc gcgccctacg ccaagggcgg gaagatcggt
     5461 ctgttcggcg gcgccggcct gggcaagacc gtcatccttc aagagctgat ccggaacatc
     5521 gcgaccgagc acggcggctt ctccgtcttc tcgggggtgg gggagcggac ccgcgagggg
     5581 aacgacctct ggctggagat gaaggagtcg ggcgtcatcg acaagaccgt gatggtgttc
     5641 ggccagatga acgagccccc cggcgcgcgg ctccgcgtcg cgctcacggg ggtcaccatg
     5701 gccgagtact tccgcgacca ggagaaccag gacgttctct tcttcgtgga caacatcttc
     5761 cgcttcacgc aggcgggctc ggaggtgtcg gcgctcctcg gccgcatgcc gagcgcggtg
     5821 ggctaccagc cgaccctttc gaccgaaatg ggcgccctgc aagagcgcat cacctccacg
     5881 aagcgcggct cgatcacgtc ggtgcaggcg atctacgtgc ccgccgacga cctgaccgat
     5941 ccggcgccgg cgaccacgtt cacgcacctc gacgcgacca cggtgctctc gcggcgcatc
     6001 tcggagctcg ggatctatcc cgccgtggat ccgctcgact ccacgtcgcg gatcctcgac
     6061 ccgaacatcg tgggcgagga gcactacaac ctggcgcgcg cggtgcagaa gatcctccag
     6121 cgctacaagg agctccagga catcatcgcc attcttggca tcgacgagct ctcggacgac
     6181 gacaagctga tcgtggcgcg cgcgcgcaag atccagcgct tcctctcgca gccgttcttc
     6241 gtggccgagc ccttcaccgg gacgcccggc aagtacgtga agctggagga cacgatccgg
     6301 tccttccagc gcatcgtcgc gggcgactgc gaccacattc cggagcaggc cttctacatg
     6361 atgggcacga tcgacgacgt gttcgccaag gccgagcaga tgggagtgac cgcgggggcc
     6421 taggccccga gcgccgctat ggccgagttc acgttccggc tggtcacgcc gcagcgcatc
     6481 gtcttcgagg gcccggtcgt ctcgatccag gcgccgggct ccgaaggcta cctcggcgtg
     6541 ctcgcccacc acgcgccgct gatcaccatg ctgaaggatg gccgtctcga gatgcgcgac
     6601 ccctccgggc gcgtcaccgc ctggcacgtg accggcggct tcctcgaggt ctcgaacaac
     6661 cgcgcgtcgg cgctcgccga ctcggcggag ccgatcacgc cctagtgcga ccgcgcccgt
     6721 cgcggcgcgg ccccgttcca ccatcggagg acagccatgt cctgggacaa gaccatcaag
     6781 tggatcatgc tggtctccgg cgcgctcacc tgcacgatgc
//
LOCUS       DASSLZ010000278         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_610113, whole genome shotgun sequence.
ACCESSION   DASSLZ010000278 DASSLZ010000000
VERSION     DASSLZ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610113"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFM00_10565"
     CDS             complement(<1..838)
                     /locus_tag="VFM00_10565"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET7498733.1"
                     /translation="MPRTALGAASVAALLALWVYVQTLSPTVAWVNQGEDSGDLLAAA
                     ATLGIAHPTGYPLFVLLGRVASLVPFGATAFRINLVAALAGAASVFFLALLVVELASP
                     RRERGAIDSPVDPGAVPSPGASGVPALVAAFAAALAYAFSRGAWSQSVLAEVYTLNAA
                     FLGAILWASARADRTGSARHLVLAAFLLGLGLTNHLLLLAAGAAVFVAAVRLASRRSI
                     GPFEVVLSLLLFVWGATLVLYLPIRALRVPEFSWGAPNTPERLWWVLSGAQYRNHFFG
                     RGP"
     gene            1140..2168
                     /gene="pilM"
                     /locus_tag="VFM00_10570"
     CDS             1140..2168
                     /gene="pilM"
                     /locus_tag="VFM00_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET7498734.1"
                     /translation="MFGRKKSTVGLDIGSSSIKVAEIVQDRGGDRLVNYGISEPLSEA
                     IVDGEIMDRQMVHEAITNLIESRQIKNKNVVTAVSGRAVIVKKILMDRLSEEDAKEAI
                     QWEAEQHVPYDINDVSLDFQIINPNVDQKKMQVLLVAAKKDMIANHAEIIKEAGLTPS
                     IIDVDSFAIQNAIEANYELHADETVALINIGAEITNLNIVRNQTPHFTKDLSVGANSF
                     VEGIQRKYNVSQAEALSALRGRGEGAIDVASIVQATCEDLSVQIDRAVAYLRSAGDAD
                     RVDRILISGGSARVPGLAEFLNSRHQVPVEIVNPLQRLLVDPGLFDGDSPDGVGPVLT
                     VAIGLALR"
     gene            2179..2730
                     /locus_tag="VFM00_10575"
     CDS             2179..2730
                     /locus_tag="VFM00_10575"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET7498735.1"
                     /translation="MIRINLLPKDERQVRRSFQFPKVGAVMPVLVLLLVAALFTAFSV
                     VQAMQVSRLKSDIARATAEADKLRPQLQMISELTTKREELTRRLNVITELDKTRLWRV
                     RLVDELSKCVPDHLWLTTYEETGPNTVQVEGVTFSNLLVADFMSRMEASPLYGNVDLV
                     VAEKGTIDQRSVVKFKITASMTL"
     gene            2751..3362
                     /gene="pilO"
                     /locus_tag="VFM00_10580"
     CDS             2751..3362
                     /gene="pilO"
                     /locus_tag="VFM00_10580"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET7498736.1"
                     /translation="MALDLKDPKTQKMLLVGLGTAALVYLYVFADFVPFNYKARSRQI
                     GTLKAEYTQKANELSKAQQLVNRLPELKKEFALLNQRWAIAQELLPSQKEVASLLRKV
                     TLAGQEAGVKFVLFKPGEPQKQTYFTENPVQVTVTGGFHHAGTFFSEIAELSRLVNVS
                     QLKLHGFDKGDVDDTVQADFIATAYTLAEGTPHEPAKPGAATK"
BASE COUNT          664 a         1142 c         1203 g          487 t
ORIGIN      
        1 ccggaccgcg gccgaagaag tggtttcggt attgcgcgcc gctcagcacc caccagagcc
       61 gctcgggggt gttcggggct ccccaggaga actcgggcac ccgaagggcc cggatcggta
      121 gatagagcac cagggtggcc ccccacacga agagaagaag cgaaagaacg acctcaaagg
      181 ggccgatgct ccggcgtgag gcgagacgga ccgcggcgac gaacaccgcg gctcccgccg
      241 ccagcagcaa gaggtggttc gtcagtccca ggccgagcag gaacgcggcg aggacgagat
      301 ggcgggcgct gccggtgcgg tccgcccggg ccgaggccca gaggatcgcg ccgaggaacg
      361 ccgcgttcag cgtgtagacc tcggccagca cgctctggct ccaggcgccg cgcgaaaagg
      421 cgtaggcgag cgcggcggcg aacgccgcga ccagggccgg gacgccggac gcgccggggc
      481 tcgggacggc gccgggatcg acgggggaat cgatcgcccc gcgctcgcga cgcggcgacg
      541 ccagctcgac cacgaggagc gccaggaaga agacgctggc cgcgcccgcg agggcggcga
      601 cgagattgat gcggaaggcg gtcgcgccga agggaaccag cgacgcgacg cgtccgagca
      661 ggacgaagag gggatagccg gtagggtgcg cgatgcccag cgtggcggcc gcggcgagaa
      721 gatcgccgga gtcctccccc tggttcaccc acgcgaccgt gggcgacagg gtctggacgt
      781 atacccagag cgccagaagg gcggcgaccg atgcggcgcc gagggcggtc cggggcatgc
      841 ggcgcaacgt aacagacgct tcgccggcgg gtcaaacgcg cgccttcggc accggatgaa
      901 gccgggccga ctcgcgattc ggccgaacaa agagtctccg gacgtgcgga ttgcacgggg
      961 cggcgggagg ctcgatgcag agcgcggcgc accaggcagg gccgcgtaaa cgatttgctt
     1021 caaatggctt acattgcggg tgcgggacac cgtggcatgg aagttgctcg atagcagcca
     1081 cggctcttcc cgcagtcggg tgcgggatcg ccacatccca ccacatcgcg gagcacgaca
     1141 tgttcggcag gaagaagagc acggtgggtc tcgacatcgg atcgagctcg atcaaggtgg
     1201 ccgagatcgt gcaggaccgc ggcggcgacc gcctggtgaa ctacggcatc tccgagccgc
     1261 tcagcgaagc gatcgtggat ggcgagatca tggaccgcca gatggtgcac gaggcgatca
     1321 ccaatctcat cgaatcccgc cagatcaaga acaagaacgt cgtgaccgcg gtctccggcc
     1381 gcgccgtcat cgtgaagaag atcctgatgg accggctgag cgaggaagac gccaaggaag
     1441 cgatccagtg ggaggcggag cagcacgtcc cctacgacat caacgacgtc tcgctcgact
     1501 tccagatcat caatcccaac gtcgaccaga agaagatgca ggtgctgctg gtcgccgcca
     1561 agaaggacat gatcgcgaac cacgccgaga tcatcaagga ggccggcctc acgccctcga
     1621 tcatcgacgt ggactcgttc gcgatccaga acgcgatcga ggccaactac gagctccacg
     1681 ccgacgagac cgtcgcgctg atcaacatcg gggccgagat caccaacctg aacatcgtgc
     1741 ggaaccagac gccccacttc acgaaggacc tctcggtggg cgcgaactcc ttcgtcgagg
     1801 ggatccagcg caagtacaac gtgagccagg cggaggcgct gagcgcgctc cgcggccgcg
     1861 gcgagggcgc gatcgacgtc gcgtccatcg tgcaggcgac ctgcgaggat ctctcggtcc
     1921 agatcgaccg ggccgtcgcc tatctccgct ccgccggcga cgccgatcgc gtcgaccgga
     1981 tcctcatctc gggcgggagc gcccgggtgc cgggcctggc cgagttcctg aactcgcgcc
     2041 atcaggtccc ggtggagatc gtgaatccgc tccagcggct gctcgtggat cccggtctct
     2101 tcgacggcga ctcgcccgac ggcgtgggcc ccgtgctcac cgtggcgatc ggcctcgcgc
     2161 tgcgataggg ggacgctcat gatccgcatc aatctgctgc ccaaggacga gcgacaggtc
     2221 cggcggagct tccagttccc gaaggtgggc gccgtcatgc cggtgctcgt cctgctcctg
     2281 gtcgccgccc tcttcaccgc gttctccgtc gtgcaggcga tgcaggtgag ccggctcaag
     2341 tccgacatcg cccgcgccac cgccgaggcc gacaagctcc gtccgcagct ccagatgatc
     2401 tcggagctga cgacgaagcg cgaggagctg acgcgccgcc tgaacgtcat caccgagctg
     2461 gacaagacgc ggctgtggcg cgtccggctc gtggacgagc tgtcgaagtg cgtccccgac
     2521 cacctctggc tgacgacgta cgaggagacg ggaccgaaca cggtgcaggt cgagggggtc
     2581 acgttctcga acctgctcgt ggccgacttc atgtcgcgca tggaggcgtc gcccctctac
     2641 gggaacgtcg acctggtcgt ggccgagaaa ggcacgatcg accagcgcag cgtggtgaag
     2701 ttcaagatca cggcgtcgat gacgctgtaa gcccgcgagg agcgaacgcg atggcattgg
     2761 acctgaaaga tccgaaaacg cagaagatgc tcctggtggg gctgggcacc gccgcgctcg
     2821 tttatctgta cgtcttcgcg gacttcgtgc cgttcaacta caaggcccgc tcgcgccaga
     2881 tcggaacgct caaggccgag tacacgcaga aggccaacga gctgtccaag gcgcagcagc
     2941 tggtgaaccg cctgcccgag ctgaagaagg agttcgcgct cctgaaccag cggtgggcga
     3001 tcgcgcagga gctgttgccg agccagaagg aagtcgcgag cctgctccgc aaggtcaccc
     3061 tcgcggggca ggaggccggc gtcaagttcg tgctcttcaa gccgggcgag ccgcagaagc
     3121 agacgtactt cacggagaat cccgtgcagg tcacggtgac cgggggcttc catcacgccg
     3181 gcaccttctt cagcgagatc gccgagcttt cacggctcgt gaacgtttcg cagctcaagc
     3241 tccatggatt cgacaagggc gatgtcgacg acaccgttca ggccgatttc atcgcgaccg
     3301 cctacacgtt ggccgagggg actcctcatg aacctgccaa gcctggcgct gccacgaagt
     3361 aagttcttcc agagccgact ggggatcctg gtcctggctc tggtcgccgt cctcctgggc
     3421 ggcgcggcct tcttcgcgtt gcgcccgctg gcgccgccga ccttgccgac gccggcaacg
     3481 tcgcccgcgg cccagg
//
LOCUS       DASSLZ010000279         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_483410, whole genome shotgun sequence.
ACCESSION   DASSLZ010000279 DASSLZ010000000
VERSION     DASSLZ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483410"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFM00_10585"
     CDS             complement(<1..324)
                     /locus_tag="VFM00_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7498737.1"
                     /translation="MATTSSPTETSDRALPILERPVPHIIAHDVRGAHTGWTSWFTTT
                     DHKKIGIMYLYTVLVFFVLGGVEALLIRLQLGAPDNTLVTPEVYNQLFTMHGTTMIFL
                     VIVPVW"
     gene            complement(327..1274)
                     /locus_tag="VFM00_10590"
     CDS             complement(327..1274)
                     /locus_tag="VFM00_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7498738.1"
                     /translation="MARARHSSASALHILRRRMLKVLLTRGLTAALAGLALAPAAASA
                     GIFFPEKGGSENADKIWTLYLLIFILAWIVFLGVAGALLWALVNFRARKGMVAAQIHG
                     NTRLEIGWTVGAGVILIFITIFTFIMLPGIKNPPASDIDANGNPVTASNVLYASTDQP
                     NPPKGSPFLNLKVDGQQYVWRYQYPKQGSNPEIFSYVDMVVPVGMTITLDITADDVIH
                     SWWIPKLGGKMDAVPGYVNKSWFKIPESAIPEGEDQVVYNGQCAELCGRNHANMLGRV
                     IGMRYPDYQAWVQRKAEQIKTAREQAAKTREEIDKQNSQ"
     gene            complement(1280..2071)
                     /locus_tag="VFM00_10595"
     CDS             complement(1280..2071)
                     /locus_tag="VFM00_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7498739.1"
                     /translation="MEYETVQVERRGGELRITLDRPEALNAWNKQFGLDLLAAVEQAA
                     GDDEVRAVVITGAGRAFSSGADLKAGFDPTPSGHPDVHTVLTERYHPIITGIRRMPKP
                     VLAAVNGPAVGIGLSLALAADLVIARESAYFLLAFVNIGLVPDGGSSLFVPERVGFTR
                     ASEMAMLGEKVGARQALEWGLINRVAADDEFEAEVDALAQRLATGPTRSYAGTKRQLN
                     AWLYARMDEQLELEASLQQQSAESGDFLEGVQAFLEKRPPAFGGR"
     gene            2091..2630
                     /locus_tag="VFM00_10600"
     CDS             2091..2630
                     /locus_tag="VFM00_10600"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET7498740.1"
                     /translation="MDPAFVFGYGSLLARGEGTPCRLRGHRRRWGVAMDNRRTIPGYK
                     YFLEPRGGRPEVFVAFLDAVPEPGASCAGLAFRVDAAALGELDARERNYRRVEVTALL
                     DADLGGRVWAYLGLEEARERLAVGVRAGTAVVSRAYVEGVQEGFAAFGLDFDTEPELP
                     VRDLTLVRVPGPEAVHPPG"
     gene            2644..>3299
                     /locus_tag="VFM00_10605"
     CDS             2644..>3299
                     /locus_tag="VFM00_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HET7498741.1"
                     /translation="MRLPTAIRGAAGAALVAGIAAPLLRRRLKLPPPVVTGTAALAPF
                     ALCVLVPRSRKRDFAACVLQMWAYIATYEMPNDDPEALERRVRVAYPVRADKAIGLGT
                     TPTLRLQRALATPGSFRAWEKLLVWSHWLWFLFPHGTVAYLLLRRPEAFPRGAAQVYA
                     TFDIGLIGYWAIPTAPPWYAAAQGLMADGRTPELRRMMVEYGEQFWKSGWAPLYGFLG
                     G"
BASE COUNT          435 a         1117 c         1222 g          525 t
ORIGIN      
        1 ccagaccggg acgatgacca ggaagatcat cgtcgtgccg tgcatggtga acagctggtt
       61 gtagacctcg ggggtcacca gcgtgttgtc cggcgcgccc agctgcaggc ggatcagcag
      121 cgcctcgacc ccgcccagca cgaagaagac gaggaccgtg tagaggtaca tgatcccgat
      181 cttcttgtga tcggtggtgg tgaaccacga ggtccagccc gtgtgcgcgc cacggacgtc
      241 gtgggcgatg atgtggggga ccggccgctc gaggatcggc agcgcgcggt ctgaggtctc
      301 ggtgggggag gaagtcgtgg ccatgcctac tgggagttct gcttgtcgat ctcttcgcgg
      361 gtcttcgccg cctgctcgcg cgccgtcttg atctgctccg ccttgcgctg gacccaggcc
      421 tggtagtccg ggtagcgcat gccgatcacg cggccgagca tgttggcgtg gttgcgcccg
      481 cagagctccg cgcactggcc gttgtagacc acctggtcct caccttccgg gatcgcggac
      541 tcggggatct tgaaccagct cttgttgacg tagcccggga cggcgtccat cttgccgccc
      601 agcttcggga tccaccatga gtggatgacg tcgtcggccg tgatgtcgag cgtgatggtc
      661 atgccgaccg ggacgaccat gtcgacgtag ctgaagatct cggggttgct gccctgcttg
      721 gggtactggt agcgccacac gtactgctgg ccgtccacct tgaggttcag gaacgggctg
      781 cccttgggcg ggttcggctg gtccgtggag gcgtagagca cgttggacgc cgtgaccggg
      841 ttgccgttgg cgtcgatgtc cgacgccggc gggttcttga tgcccggcag catgatgaac
      901 gtgaagatcg tgatgaagat caggatcacg ccggcgccga cggtccagcc gatctccagc
      961 cgcgtgttgc cgtggatctg ggccgcgacc atgcccttgc gggcgcggaa gttgaccagc
     1021 gcccacagca gcgccccggc cacgccgagg aacacgatcc aggcgaggat gaagatcagc
     1081 aggtagagcg tccagatctt gtccgcgttc tccgagccgc ccttctcggg gaagaagatc
     1141 cccgcgctcg cggccgcggg ggccagcgca aggccggcca gggccgcggt caggccgcgg
     1201 gtgaggagca ccttcagcat gcggcggcgg agtatatgga gcgcggaggc cgaagagtgg
     1261 cgtgcccgcg ccatcgcgct catcgaccgc cgaacgccgg cggtcgcttc tcgaggaacg
     1321 cctgcacgcc ctccaggaag tccccgctct ccgcggactg ttgctggagg gaggcctcga
     1381 gctcgagctg ctcgtccatg cgcgcgtaca gccaggcgtt gagctggcgc ttggtgccgg
     1441 cgtaggagcg ggtcgggccg gtggcgagcc gctgggccag cgcgtcgacc tcggcctcga
     1501 actcgtcgtc ggccgcgacg cggttgatca ggccccactc gagcgcctgc cgcgcgccga
     1561 ccttctcgcc cagcatcgcc atctcgctcg cgcgcgtgaa gcccacgcgc tcggggacga
     1621 acagcgacga gccgccgtcg gggacgagcc cgatgttcac gaaggccagc aggaagtagg
     1681 cgctctcgcg cgcgatcacg aggtcggcgg cgagggcgag cgagagcccg atgccgaccg
     1741 ccgggccgtt gacggccgcc agcacgggct tgggcatgcg ccggatcccg gtgatgatcg
     1801 ggtggtagcg ctcggtgagg accgtgtgga cgtccgggtg accggacggc gtggggtcga
     1861 agcccgcctt gaggtcggcg cccgacgaga acgccctgcc ggcgcccgtg atcacgaccg
     1921 cgcggacctc gtcgtcgccg gcggcctgct cgaccgcggc cagcaggtcc aggccgaact
     1981 gcttgttcca ggcgttcagg gcttcagggc gatcgagcgt gatgcgcagc tcgccgccgc
     2041 gccgctcgac ctgcacggtc tcgtattcca tggggcgcga tcctacgggc atggacccgg
     2101 ccttcgtctt cggctacggc tcgctgctgg cgcgcgggga gggcacgccc tgccggctgc
     2161 gcgggcaccg ccgccgctgg ggcgtggcga tggacaaccg gcgcacgatc ccgggctaca
     2221 agtacttcct cgagccgcgc ggcgggcgcc cggaggtgtt cgtggccttc ctcgacgccg
     2281 tgccggagcc gggggcctcg tgcgccggcc tggccttccg ggtcgacgcg gcggcgctcg
     2341 gcgagctgga cgcccgcgag cgcaactacc gccgcgtgga agtgacggcg ctgctcgacg
     2401 ccgacctcgg cgggcgcgtg tgggcctacc tggggctgga ggaggcgcgc gagcgcctcg
     2461 cggtgggcgt gcgggcgggg accgcggtcg tctcgcgcgc gtacgtggag ggtgtgcagg
     2521 aaggcttcgc ggcgttcggc ctggacttcg acacggagcc ggagctgccg gtgcgggatc
     2581 tgacgctggt gcgggttccc ggccccgaag ccgtccaccc gccgggctag actcgccagc
     2641 gccgtgcgcc tgcccaccgc catccgcggg gccgcgggtg cggccctcgt cgccgggatc
     2701 gcggcgccgc tcctccgcag gcggctgaag ctgccgcctc ccgtggtgac gggtaccgcg
     2761 gccctggccc cgttcgccct ctgcgtcctg gtcccgcgct cgcgcaagcg cgacttcgcc
     2821 gcgtgcgtgc tgcagatgtg ggcctacatc gccacgtacg agatgcccaa cgacgatccg
     2881 gaggcgctcg agcggcgggt gcgggtcgcc tatccggtgc gcgccgacaa ggcgatcggg
     2941 ctcggcacga cgccgaccct gcggctccag cgcgcgctcg ccaccccggg cagcttccgc
     3001 gcttgggaga agctgctcgt gtggtcgcac tggctgtggt tcctgttccc gcacggcacc
     3061 gtcgcctacc tgctcctgcg ccgccccgag gccttcccgc gcggcgccgc gcaggtctac
     3121 gccacgttcg acatcggact gatcggctac tgggcgatcc ccaccgctcc gccctggtac
     3181 gcggccgccc agggactgat ggcggacggc cggacgccgg agctgcggcg gatgatggtc
     3241 gaatacggtg agcagttctg gaagtccggg tgggcgcctc tgtacggttt cctgggagg
//
LOCUS       DASSLZ010000280        21385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_357661, whole genome shotgun sequence.
ACCESSION   DASSLZ010000280 DASSLZ010000000
VERSION     DASSLZ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 21385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21385
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357661"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VFM00_10610"
     CDS             <1..724
                     /locus_tag="VFM00_10610"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7498742.1"
                     /translation="DAVELLEISLEEGIDTIITSAGNPAVLGPRIKGARATWVHVVPS
                     VKHGKKAQDSGADIVVGEGFEAGGHNGVDEITTLALIPQLVDALSVPVAAAGGIADGR
                     GFLAAMALGAEGISVGTRFACTPESSSHPLYKEAVVAAGDAGTVLFAKRIAPVRAIKN
                     PWVERVRQAEAQGASREALEEVYGRSHSRRGILEGDVDEGELEAGQSSGLIHDVIPAA
                     QVVRRFVDEFEQALSRVRGVGS"
     gene            721..1191
                     /locus_tag="VFM00_10615"
     CDS             721..1191
                     /locus_tag="VFM00_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498743.1"
                     /translation="MSDPKIVEEQLAQKEAPTAETAMVAGRSRYDAAMKKDNDSARCL
                     DLLEAAHQFQRAIQIAPDAPEAYGWLAQTYRMMAAASRAADSDRADLFTRFACAVAWE
                     GTLRSTPTSVTIRTKQEVRTLVAWIRATRHLSSADAEAEMKTIHGQSLEQALSY"
     gene            1436..1888
                     /locus_tag="VFM00_10620"
     CDS             1436..1888
                     /locus_tag="VFM00_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498744.1"
                     /translation="MKTTVRTEVTPSAAWRFHFRFRRPGIPAVDQPVQVLLDSIGHLA
                     PLVLRVGEVLADDPIEGIPPCANDSTLCAPSLTREEAIGIARKNGISEGLGPWKVEFG
                     WNRSRRRFMWILTNTLIDRGPCDRSGKVVFIDASTGEIIWGGAWSQEC"
     gene            complement(1896..2711)
                     /locus_tag="VFM00_10625"
     CDS             complement(1896..2711)
                     /locus_tag="VFM00_10625"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7498745.1"
                     /translation="MSGPFYERELAAAVAIAHEAGALLKENFGRQQEIGYKGRINLVT
                     EMDKRSERLIISRLNAAFPGDDILAEEGGGERRGSERLWIVDPLDGTTNYAHEYPVWS
                     VSIALQEAGDLKVGVVYNPLLDDMYAARRDGGTTLNGTPRRVTEVDDLGKAMLGTGFS
                     YDLSGDFESNNIGPFSRFLLKAQAVRRAGSAALAIAKVGVGRSDGFWEGGLQPWDMAA
                     GMLIVEEGGGKTSNYEGGPAKVTIRQLVATNGRIHDAMLGVVKTRDVHQRPEA"
     gene            complement(2782..3189)
                     /locus_tag="VFM00_10630"
     CDS             complement(2782..3189)
                     /locus_tag="VFM00_10630"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7498746.1"
                     /translation="MSPTDPLHPGRPTGEGEPPPEWAMRTYVLGLLYRGPNQPTDDAE
                     RDELFRSHMSNSARMYEAGKLLIAGPLMDKTDLRGIWIFDSESIEECEKLVSGDRAVT
                     AGMFRVEYHPWYSAKGITIKRPDVADIPPTTAR"
     gene            complement(3186..3677)
                     /locus_tag="VFM00_10635"
     CDS             complement(3186..3677)
                     /locus_tag="VFM00_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7498747.1"
                     /translation="MSEKTREEIEVRVRRMTEEDAPAVNELVSQLGYSSDVGKTETAI
                     RAVLGSEVGDAFVAEDPEGHVIGWAHVFMVPFIESGPNAELGGLVVDEKYRGSGAGRA
                     LVEHVEAWARERGALEVSLRTNIVRDGAHKFYQHLGYEVQKTQHKFHKSLGSGKGTKG
                     GKE"
     gene            complement(3674..5560)
                     /locus_tag="VFM00_10640"
     CDS             complement(3674..5560)
                     /locus_tag="VFM00_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7498748.1"
                     /translation="MAGPWGSASDDIDAQGAIYDRRLAGRLLRFLKPYKKEIGLSIAV
                     LGVLSLLEAAGPFLTKIAIDKYVKPAGGHGALPADAVRGLFFIAIAYVAVLAVAFVLR
                     YIQSYTMAMVGQRAMQDLRLTIFRHLETLTPSYFDTRPVGRILTRVTQDVAVLNELFA
                     QGVVAVIGDIFMLAAIVFAMFWMNWRLALVTMTTVVLLVIATAIFRSKVRVSYRRVRT
                     RLARLNAFLQEHLQGLDVLKFFNAERKQTLKFDEANRSHYDAHVQNLFYYAVFFPAVE
                     IIGAIALALILWYGGSGILAGTVTFGVVVAFIQYSDRFYQPVRDLSEKYNVFQAAMVA
                     SERIFELLDTEPAVVEPKEAPEVGRLRGDVEFDHVWFAYKDEEFVIRDLSFHIAPGEK
                     VAIVGATGAGKSSIANLLTRFYEFQRGSIRVDGRDIRTIEGKDLRRQIGLVLQDVFLF
                     SGTVRENIAFGARDRADGQVDRALMEVGGSRLHARLPHGLDEEVGERGAYFSMGERQI
                     LAFARALHYDPSILILDEATSSVDVETERIVQAALKRLLEGRTSLVIAHRLSTILDAD
                     RILVLHKGELREQGTHAELLGKGGIYARLYELQYSKESAPDVRLDGDGMEREQPVAAD
                     GEQS"
     gene            complement(5592..7571)
                     /locus_tag="VFM00_10645"
     CDS             complement(5592..7571)
                     /locus_tag="VFM00_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7498749.1"
                     /translation="MLPGPDPPRDAASALGRRDHDLRGLWPHLSLQRGQLLLVPAERP
                     VSGGRLAPASVGPASPHPARITVIGSLRHLLPYLRRHKIAIFFGTLSIGLTTVFQVWS
                     PWLVRQAVDHLQAGVTRSALLRDSGLILLAVTLQGVFLFTMRMSLIRASRRMEYELRN
                     DLFDHFTRLPAETYRTRKVGDLLSRATNDLDAVRNFLGPGIMYFGNTIATFVLAVTLM
                     CRIDLRLTLLSLIPLPILSFTVARLGAKLHHHYEAIQASFAAMTAKAQETLAGIRVVK
                     AHVEEEGEYRAFAAIHEDYLEKNRAMIKIWSGMWPMLSLLGGIASAIVLGVGGAAVVR
                     GQITLGELVAFQIYLAMLIWPMISLGWVSNLFQRGAASMGRIQEILALPVEDDVEPGT
                     PWPRPDRAAPDGASHAHVPAVALENVSFHYPGTDRDVLHALNLSIRAGETLAVVGRTG
                     SGKTTLLNLIARLYEPTGGVIRFEGHPVSELPRGPLRRRLGIVPQETFLFSDTIRANV
                     AFGFEPGAVQPDVDEVVRRAGLSSDLTQFPQGLDTMIGERGITLSGGQKQRVALARAL
                     ALERPVLLLDDAFASVDPGTEEVILDNLFRATQRATVVLATHRRSALLRVDRIVVLDE
                     GRIVASGTHEELLARGGLYGDLYHLEEVVEELEAL"
     gene            complement(7457..8173)
                     /locus_tag="VFM00_10650"
     CDS             complement(7457..8173)
                     /locus_tag="VFM00_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET7498750.1"
                     /translation="MLPELEALLELQRRDTLLLDAKRRKEAIPSRRESLRGALAAARA
                     DLERAKKELDAGRGGRRSLEKEVEAFQADVAKLERQLLDVKTNQEYTAMRHQIEGVKQ
                     KRSDVETKILESYDAEEKLVLAMKSAEKRVAEEEGRLKREEASMDQEAAALEQTLAAL
                     MQDRDAVRPRVPAPVLSRYDRLAGARDGVAVAEVKKGACGACFRALTPHAMQQVRLGD
                     AIMICEACGRILVYNEGSSS"
     gene            complement(8350..8425)
                     /locus_tag="VFM00_10655"
     tRNA            complement(8350..8425)
                     /locus_tag="VFM00_10655"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8390..8392),aa:Val,seq:cac)
     gene            complement(8493..8566)
                     /locus_tag="VFM00_10660"
     tRNA            complement(8493..8566)
                     /locus_tag="VFM00_10660"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8530..8532),aa:Ile,seq:cat)
     gene            complement(8675..10447)
                     /gene="rpoD"
                     /locus_tag="VFM00_10665"
     CDS             complement(8675..10447)
                     /gene="rpoD"
                     /locus_tag="VFM00_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET7498751.1"
                     /translation="MKRKAEGLKREKGGDRLVELKSLAGKQHYLLHDQIETFLDESAS
                     VEDMDEVYIALNDLKVDVFDTHEEAQQKLRLKRREEKRPPEKAAALPHPVRYDDPVRM
                     YLREMGRVPLLDREGEVEIAKRIKAGEKQICTALFRSGPILKEIISYGTRLKQGKMRV
                     EDLTQIDFGNWNPEFSPKRERARALKAFDKIRLLHGKFTKLQEKPTGRLSEALRATHQ
                     GKLRTADEDLMCELFTLNLNTRLIERLAERIKDYSHQIDLVESEFTDLERRWGKNGDE
                     IADLLKRAKKSKKEAKQVEKESALTPERFEEYDRASKNARRKVRKIEEEAHMTRDQVK
                     EVAREIRSGEIARDKAKREMIEANVRLVISIAKRYTNRGLEFLDLIQEGNSGLMRAVD
                     KFDYRKGYKFSTYATWWIRQAITRAIADQARTIRVPVHMIEAINKVVRTSRRLVQELG
                     REPTPEEVAAKLALPVDKIKSVLKAAQEPISLDRPIGEDDDSSLGDFIEDTSVVSPAQ
                     AAAFSMLKDEVNEVLETLSKREAKVIRLRFGLTEDGCPRTLEEVGATFNVTRERIRQI
                     EAKALRKLRHPTRSRRLKGYVEMM"
     gene            complement(10466..12322)
                     /gene="dnaG"
                     /locus_tag="VFM00_10670"
     CDS             complement(10466..12322)
                     /gene="dnaG"
                     /locus_tag="VFM00_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET7498752.1"
                     /translation="MTAMLSSEAKERVREAVDLVALVGEKVALRRAGRSWKGLCPFHA
                     ERTPSFTVNPDRQVWHCFGCGKGGDLFAWVTETEKVAFPEALRMLAERAGIELPKTAR
                     GPGDVARDRLYQANALANDYFRASLDSEAGAEAREYMTGRGFDRASLDAFQIGWAPAG
                     WDGLVTTLGKLVPVAVLEEAGLALRRADGSRYDRFRNRITFPVMESAQRTAGFGARAI
                     ALADQPKYLNSPETAVYRKGSILFGLPQARAAIRDTRQALIAEGYLDVMRLHAAGFRN
                     AVATSGTALTYDQARILARLEAEAVLVYDGDDAGVRAADRALEPLMRAGLAVRVLLLP
                     PGEDPDTFIRKEGPSAFQALLADAADAAGFLAGASLGAGGENPSVEARVRRYVEVIGR
                     SEDPIRRRMLVRRGAEAFALEEKVLLEALERKGGRAPKKPAAPAPAPDPGAKAGMASA
                     EPPEAGAPAAGPGEPGWIAPDPVESELAALVLTEEGALGEVVAKGGADCFRHQGLRDL
                     LDAWLAEVRVPGELELLDLMNASGLARAVLTGHPKPEEPLTDSESRRGARALIERLEE
                     RRIRASIQDLDRAIRQAERSHDAKDLGLLGSLVAERRDLASKLHSRSHPAVS"
     gene            complement(12319..14766)
                     /locus_tag="VFM00_10675"
     CDS             complement(12319..14766)
                     /locus_tag="VFM00_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET7498753.1"
                     /translation="MERARDHAHDVLEFPAVLRALAGRTRSVPGRERVLARAPMRRPE
                     DARAAQAETADLLAAHEAGDPPPTAAPPDLGLVLQRLETEGSTLRGEELWQIAVLLEQ
                     AGQILTWYRKRREDAPGLERLLGGLDPAEGLRRDLVRAIDPSGAVKDDASPELSKIRR
                     SIRSLRERLASKLESILRAAGSPESFVTLREERYVIAIPSSNRRSVPGAVLGHSGSGA
                     SVFVEPREAAEGNSELAERASDEAREVERILRSLSVEAHRHHGALMADLAALAGLDAA
                     EAVASWAIEVGAVLPVIADERRLLLRGARHPILVLRHVRGEMGPPVPLDLELDAENPV
                     LLVTGPNMGGKTVAMKTVGLLSLMAMAGLPVTAAHGTTFAWFDRIVCDIGDEQSIQDD
                     VSTFLSHVRRVTQALAAATDRSLVLLDELGSGTDPAEGAALGQAVLEALLQRGAMTLA
                     TTHHGSLKTFAHETAGVRNASMAFDEATLRSLFRLVVGVPGGSRAIQVAERFGMDAAV
                     LARARTLLPEGERDLNRLIEELGRLREEAVAERRRLTETQAKLRERESELRSTRERLE
                     SERKERKQAEIAARRELLRQLESQIDDYRKKVRSERKASAETLQQGRGLAKAMEESIA
                     AEDAAAQPAPAERGAPLRTVREGDKIFVPALRAEGTALSLPDKDGRVRVKIGAATAVL
                     PVSQLRSMQPSAAGAARPGGKRDERSEDEEEKDAAAQAPVRGAAIATPDLEDTGTQVD
                     VRGYEADDAVRAVERFLEDAAVGGQQTVRIIHGKGRGILREHMKRFLAHNQLVKEFRL
                     GEMGEGGTGVTIVTLEP"
     gene            complement(14930..15124)
                     /gene="rpsU"
                     /locus_tag="VFM00_10680"
     CDS             complement(14930..15124)
                     /gene="rpsU"
                     /locus_tag="VFM00_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET7498754.1"
                     /translation="MPGIRVKDGESFESALRRFKKKCEKAGILADLRRHQHFEKPSER
                     KKRKLNAAKRKMMARMKSAE"
     gene            complement(15204..16004)
                     /locus_tag="VFM00_10685"
     CDS             complement(15204..16004)
                     /locus_tag="VFM00_10685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HET7498755.1"
                     /translation="MKGAARTPDTAFWVDRANVDGTRLRLSDSESRHLLRVFRAAPGT
                     PFEAVDGEGTLYRCVLETASGGIAVGTIESRAVEAGELGAAITLLAGLPDLQQIETIV
                     SLAVPLGVTAIDFVATGHSGREPLPEARLQRLARIARSALKQSRRTRLPALRSSPGLE
                     EALQEIPVETFRLFAEPGGEPLRAEVVETMLQISQPHAVLAVGPPGGFLREEGELLTM
                     RSFRPISLGPSRLTTSTACLALLGATRNLMLTAGLGRVDKTGLSGYLQ"
     gene            complement(16015..17172)
                     /gene="dnaJ"
                     /locus_tag="VFM00_10690"
     CDS             complement(16015..17172)
                     /gene="dnaJ"
                     /locus_tag="VFM00_10690"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET7498756.1"
                     /translation="MSTVRDYYEVLGVERSASPEEIKKAYRQLALKFHPDKNPGDKQA
                     EERFKEVAEAYEVLSDPEKRARYDRFGHAPQGFPGGGGGGFEDMRGFDLADALRAFMR
                     DFGGPGGFGDLFEEGGGVGRRPRERRGNTLELRLPLTLEEIATGTEKRVKVRHLRVCK
                     TCKGTGAKEGTSRITCPKCHGSGQIRQVQRSIFGQLINVSTCDRCHGEGTIVENPCPV
                     CHGEGRVREQSEIAIKVPAGVSSGNFIPLPGMGDVGPRGGPAGDLIAHIEEIEHPLFA
                     RDGDDLVVEVPISLSRAALGGKVEVPTLGGGRALVEIPAGTGTGKLLRLRGKGLKSLQ
                     RSGTGDLLVRVVVAVPSKLSERGKKALQEFETLPEAKAPAPRRPAWTGDEG"
     gene            complement(17169..17867)
                     /locus_tag="VFM00_10695"
     CDS             complement(17169..17867)
                     /locus_tag="VFM00_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET7498757.1"
                     /translation="MKPKRAATTEDRDETRADAPDTNAAPDTGALGRDPDAVEAEIKR
                     LRDREEELLRALAEMTNVQKRRKLESEQTVRYAQESLIRELLPVLDDFERALAAMPGT
                     PHDPLHAGVELVRDRMMKILEKEGLAPIQSAGQPFDPNLHDAIGERVAPAGTRPGVVL
                     DVAQPGYMLNDRVLRHAKVVVAGPPADTTTPPSMLGESAPPDTIPPALEDRDRMGATD
                     VDRTEPSGKDRHRA"
     gene            complement(17870..18922)
                     /gene="hrcA"
                     /locus_tag="VFM00_10700"
     CDS             complement(17870..18922)
                     /gene="hrcA"
                     /locus_tag="VFM00_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET7498758.1"
                     /translation="MKPNIPHSPHSPHSRDPRLDPRLSRILTFVVESHVSRAEPVGSQ
                     YVRAAYHLSISPATIRNAMRELEDLGLLLHPHTSAGRVPTEAGYRYYVDHLMRPETVP
                     IGVRRILDEAIGRARTQSRASDLAPILARATRQLAVMAVRSREEAVDQVNLVALDGGH
                     VVVAVGARDGMVATDTWRPELFPCAADLRRAESWIRARLPAAGPKALDALVLEARAGA
                     PLEIASFIEEALTRASRLLSTREEPAVRVDGADNIASQPEFQAPGTLRPLVSLLANQE
                     SLVRTLDAFSGESVPRVAIGREIESPAIRACSIVGMGVAVGGLRGAIGVMGPVRMPYR
                     RVISLVSYVGARLAGA"
     gene            complement(19019..20212)
                     /gene="hemW"
                     /locus_tag="VFM00_10705"
     CDS             complement(19019..20212)
                     /gene="hemW"
                     /locus_tag="VFM00_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET7498759.1"
                     /translation="MDYGLYVHLPYCRSLCPYCAFSKAPLHHAQPERLLRALAREWEL
                     AAAEAPGPWVENRPRTIFVGGGTPTALDEKTLAAFLDWVRSAFPAARIREWTVEANPE
                     GLTAEKLRLLRRAGVDRLSLGVQSFEAEALRALGRIHTAQGALDALARARAAGFTNVS
                     LDLMAGVPGETADGFARGVERVIALSVPHLSVYSLQVEEGTPLAAKAARGAIAIPAED
                     EAAERYDVAERLLSAAGYQRYEVSSWALPGFASRHNQSYWIRRPYLGLGPGAHSFDGS
                     RRWGNEEDVARWFDRVEAGALPRHDVRALSEADAREEVVFLGLRRARGLRRAALDRAA
                     PYEAEGWLRWGEAAGALRSGRGTVRPTERGLLTALESAAELFARAGARAGARAAARAG
                     AGPPG"
     gene            complement(20250..20918)
                     /gene="lepB"
                     /locus_tag="VFM00_10710"
     CDS             complement(20250..20918)
                     /gene="lepB"
                     /locus_tag="VFM00_10710"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7498760.1"
                     /translation="MTNGSSVAAARARARTKSALREYLEAILLAIVLTIVIRGLVLQA
                     FRIPTGSMEDTLQVGDFLFVNKMVYGSEIDIGFGGERFFYYRFPTIREPKPGDVVVFR
                     YPEDPSRDFIKRCVAVAGQTVEIRDKVLYVDGKAPVEPYVVHKDPRIIPRETYPRDNY
                     GPYVVPQGHLFMMGDNRDNSQDSRFWGALPRELVKGKAMFIYWSWDQDRRLPRLNRLF
                     RPIH"
     gene            complement(20946..>21385)
                     /locus_tag="VFM00_10715"
     CDS             complement(20946..>21385)
                     /locus_tag="VFM00_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350019.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET7498761.1"
                     /translation="LDFYDRLKSISRGYASLDYELAGYKPAELVKMDVLLNGDPVDAL
                     SVIVHREKAYDWGRDISVKLKELIPRQMFPIAIQAAIGGQVIARETISALRKNVIAKC
                     YGGDISRKRKLLEKQKEGKQRMKQVGTVRIPQEAFLAVLRVER"
BASE COUNT         3179 a         7572 c         7171 g         3463 t
ORIGIN      
        1 ggacgccgtc gagcttctgg aaatctcgtt ggaagaaggc atcgacacca tcatcaccag
       61 cgcgggaaat cccgccgtgc tgggcccgcg catcaagggc gcgcgggcga catgggtcca
      121 cgtggtgccc agcgtgaagc atgggaagaa agcgcaggat tcgggagccg acatcgtggt
      181 cggggagggc ttcgaggcgg gcggccacaa cggcgtggac gagatcacga cgctggcgct
      241 gatcccccag ctggtcgacg cgctgtccgt gccggtcgcg gcggccggtg gaatcgcaga
      301 cggccgcggg ttcctggccg ccatggcttt aggggcggag ggcatctccg tgggcacacg
      361 ctttgcatgt actccagagt cgtcttccca ccccctttat aaggaagcgg ttgtcgccgc
      421 aggcgatgcc gggaccgtgc tgtttgcaaa gcgcatcgcg ccagttcgcg cgatcaagaa
      481 cccctgggtg gaacgggtcc gccaggccga agcccagggg gcgtcgcgag aggcgctgga
      541 agaggtgtac ggccgatccc attcgcgccg gggcatcctc gaaggggacg tggacgaggg
      601 cgagctggag gccggtcagt cgagcggatt gatccacgac gtgatcccgg ccgcccaggt
      661 cgtgcggcgg ttcgtcgatg aattcgagca ggccctgtcc cgagtgcgag gcgtcggatc
      721 atgagcgacc cgaaaatcgt cgaggagcag ctcgcgcaga aggaagcccc caccgccgag
      781 accgcgatgg ttgcggggcg gagtcgctac gacgccgcga tgaagaagga caacgacagc
      841 gcccgctgtc tcgatctcct ggaagccgcc caccagttcc agcgcgccat ccagatcgcc
      901 cccgacgctc ccgaagccta cggatggctg gctcagacct accggatgat ggccgccgcc
      961 tcgcgcgccg ccgactccga ccgcgccgat ctgttcaccc gattcgcctg cgccgtggcg
     1021 tgggaaggaa ccctgcgaag cacgccgacg tccgtgacga tccggaccaa gcaggaggtc
     1081 cggaccctcg tggcgtggat ccgcgcgacc cggcacctat cgtcggcgga tgcggaagcg
     1141 gagatgaaga cgattcacgg gcagagtctg gagcaggcgc tcagttacta gaaattcagc
     1201 cacggtgaat tgaaatcaga gtagcctagg ggcgttgggc gcccgccggc cccgagcacc
     1261 tatgcccctt cgccctgccg ttctcagtgt agccctgctc tgcgggtctt ccgcatccgg
     1321 cagcgcctcc tcgcaagtac ccccgcctgc ggtcgtcgat agcgcctgga cccacctcgc
     1381 ccgatatgcg ggcccatccc cgatgcggta catcacgctc gagacggttg acagcgtgaa
     1441 gacgacggtc agaacggaag tcacgccatc agcagcatgg cgattccact ttcgctttcg
     1501 acgccccggg ataccggcag tggatcaacc tgttcaggtc ctgctcgact ccatcggcca
     1561 cctggcgccc ttggtactcc gagtcggaga ggtccttgcg gatgatccca tcgaaggcat
     1621 ccctccttgc gcaaacgatt caactctttg cgcgccgtcg ctcacacggg aagaggcgat
     1681 cggaatcgcg cggaagaacg gaatctccga ggggctgggg ccttggaagg tcgagtttgg
     1741 ctggaatcga tcccgcagga gattcatgtg gatcctgacc aacacgctca ttgaccgagg
     1801 gccttgtgat cggtcgggta aggtcgtctt catcgatgcc agcaccggcg agatcatctg
     1861 gggcggtgct tggtctcagg agtgctgagt cggcatcacg cctcgggccg ttggtggacg
     1921 tcccgggtct tgaccacccc gagcatcgcg tcatgaatgc ggccgttggt cgccacgagc
     1981 tggcgaatgg taaccttcgc gggaccgcct tcgtaattgc tcgtcttccc gccgccctcc
     2041 tccacgatca acattccggc cgccatgtcc cagggctgga gtccgccttc ccagaagccg
     2101 tcgctgcgcc cgacgccgac tttcgcgatc gctagcgcgg cggagccggc gcggcgcacg
     2161 gcttgcgctt tcaggagaaa gcgggagaac gggccgatgt tattcgattc gaaatcgccg
     2221 ctcaggtcgt aggagaagcc ggttccgagc atggccttgc ccaggtcgtc gacttcggtg
     2281 acgcggcggg gcgttccgtt cagcgtggtg ccaccgtcac gtcttgcggc gtacatgtcg
     2341 tccaggagcg ggttgtagac gacgcccacc ttgaggtcgc cggcttcctg gagcgcgatg
     2401 gagacggacc agacggggta ttcgtgggcg tagttggtgg tcccgtccag ggggtcgacg
     2461 atccagaggc gctcggagcc gcggcgctcg ccgcctccct cttcggccag gatgtcgtcg
     2521 cctggaaatg ccgcgttcag cctggagatg atcaagcgct cggaacgctt atccatctcg
     2581 gtgacgaggt tgattcggcc cttgtagccg atctcctgct ggcggccgaa gttctccttc
     2641 aagagcgcgc cggcttcgtg cgcgatggcg actgcggccg ccagctctcg ttcatagaac
     2701 gggccgctca tcgatacgct cgggtgatgg agaagagcaa cttgcgctgg cgcaagttaa
     2761 catacatgtt acgtgccaac gtcagcgggc tgtggtcgga ggaatatccg cgacgtcggg
     2821 gcgcttgatg gtgatgccct tggccgagta ccaggggtgg tactccacgc ggaacatgcc
     2881 cgcggtcacc gcgcggtcgc ccgagacgag cttctcgcac tcctcgatgg attcggaatc
     2941 gaagatccag atgccgcgca ggtcggtctt gtccatcaag ggaccggcga tgaggagctt
     3001 tccggcttcg tacatccggg cgctgttgga catgtggctg cggaagagct cgtcccgctc
     3061 ggcgtcgtcc gtcggctggt tcggtccgcg gtacaggagg cccagcacgt aggtgcgcat
     3121 cgcccattcg ggaggcggct cgccttctcc ggtcgggcgt ccgggatgca gcggatccgt
     3181 cggactcatt ccttccctcc cttggtgccc tttcccgacc ccagcgactt gtggaacttg
     3241 tgctgcgtct tctgcacttc gtagccgaga tgctggtaga acttgtgggc gccatcgcgc
     3301 acgatgttcg tgcgcagcga gacctcgagg gcgccgcgct cccgcgccca cgcctcgacg
     3361 tgctcgacca gcgcgcgtcc ggcccccgat ccgcgatact tctcgtccac gaccaggccg
     3421 cccagctcgg cgttcggtcc cgactcgatg aacgggacca tgaacacgtg ggcccagccg
     3481 atgacgtgcc cctcggggtc ctcggccacg aaggcgtccc ccacctcgga gcccagcacg
     3541 gcgcggatcg cggtctcggt cttgcccacg tcgctggagt agccgagctg gctgaccagc
     3601 tcgttcaccg cgggcgcgtc ctcctcggtc atccggcgca cgcgcacctc gatctcctcg
     3661 cgggtcttct cgctcacgac tgctccccgt cggcagccac cggctgctcg cgctccattc
     3721 cgtcgccgtc caaccgcacg tccggcgccg actccttgct gtactgcagc tcgtagagcc
     3781 gggcgtagat accgcccttg ccgagcagct cggcgtgcgt tccctgttcg cgcagctcgc
     3841 ccttgtggag gacgaggatg cgatcggcgt ccaggatggt gctcagccgg tgggcaatca
     3901 cgagcgacgt gcgcccttcc aacagccgct tcagcgcggc ctgcacgatc cgctccgtct
     3961 ccacgtccac gctcgacgtg gcctcgtcca gaatcaggat gctcggatcg tagtggagcg
     4021 cgcgcgcgaa ggcgaggatc tgccgctcgc ccatcgagaa ataggctccg cgctccccca
     4081 cctcttcgtc cagtccgtgc ggcagccgcg cgtgcaggcg cgagccgccc acttccatca
     4141 gggcccggtc cacctgtccg tccgcgcggt cccgcgcgcc gaacgcgatg ttctcgcgca
     4201 cggtgcccga gaagaggaac acgtcctgca gcaccaggcc gatctggcgg cgcaggtcct
     4261 tcccctcgat cgtgcggatg tcgcgcccgt ccacgcggat cgagccgcgc tggaattcgt
     4321 agaaccgggt gagcaggttc gcgatcgagg acttcccggc gccggtcgcc cccacgatcg
     4381 ccaccttctc gcccggcgcg atgtggaagg acaggtcgcg gatcacgaac tcctcgtcct
     4441 tgtaggcgaa ccagacgtga tcgaactcca cgtcgccgcg caggcgcccc acctcgggag
     4501 cctccttcgg ctccaccacg gcgggctcgg tgtcgagcag ctcgaagatc cgctccgagg
     4561 cgaccatcgc cgcctggaag acgttgtact tctcgctcag gtcgcgcacc ggctggtaga
     4621 agcggtcgga gtactggatg aacgcgacca cgacgccgaa cgtcaccgtc ccggcgagga
     4681 tgccgctgcc gccataccac aggatcagcg ccagcgcgat cgcgccgatg atctcgacgg
     4741 ccgggaagaa gaccgcgtag tagaagaggt tctgcacgtg cgcgtcgtag tgcgagcggt
     4801 tggcctcgtc gaacttcaag gtctgcttcc gctcggcgtt gaagaacttg agcacgtcca
     4861 gcccctggag gtgctcctgc aggaaggcgt tcaggcgcgc gaggcgcgtg cgcacgcgcc
     4921 ggtaggacac gcgcaccttg gaccggaaga tcgccgtggc gatcaccagc agcacgaccg
     4981 tggtcatcgt caccagggcg agccgccagt tcatccagaa catggcgaag acgatcgcgg
     5041 cgagcatgaa gatgtcgccg atcacggcga ccacgccctg cgcgaagagc tcgttcagca
     5101 ccgccacgtc ctgcgtgacg cgcgtgagga tgcgccccac cggccgggtg tcgaagtagg
     5161 agggggtcag cgtctcgagg tgccggaaga tggtgagccg gagatcctgc atcgcccgct
     5221 gccccaccat cgccatcgtg taggactgga tgtagcggag cacgaaggcg accgcgagca
     5281 cggccacgta ggcgatcgcg atgaagaaca gcccgcggac cgcgtcggcc ggaagcgccc
     5341 cgtggccgcc ggcgggcttc acgtacttgt cgatcgcgat cttggtcagg aacgggccgg
     5401 ccgcctccag gagcgagagc acgccgagga cggcgatcga gagcccgatc tccttcttgt
     5461 acggcttcag gaagcggagg agacggcccg cgaggcggcg gtcgtagatc gcgccctgcg
     5521 cgtcgatgtc gtccgaggcg gagccccagg gtccggccat cagcggctcc tttccatgcg
     5581 gcgcgcccgg ctcatagggc ctcgagctct tccaccacct cttccaggtg gtagaggtcg
     5641 ccgtacagcc cgccgcgcgc gagcagctct tcatgggttc cggacgcaac gatcctgccc
     5701 tcgtcgagca cgacgatccg atccacgcgg aggagcgccg aacggcggtg cgtcgcgagc
     5761 acgaccgtgg cgcgctgcgt cgcgcggaac agattgtcca ggatcacctc ttcggtgccg
     5821 ggatccacgc tcgcgaaggc gtcgtccagg agcagcaccg ggcgctccag cgcgagcgcg
     5881 cgggccagcg cgaccctctg cttctgtccg cccgagagcg tgatgccgcg ctcgccgatc
     5941 atggtgtcca gcccctgcgg gaactgggtg aggtcggacg agaggccggc gcggcgcacg
     6001 acttcgtcca cgtccggctg aaccgctccc ggctcgaacc cgaacgccac gttcgcacgg
     6061 atggtgtcgg agaagaggaa ggtctcctgc ggcacgatgc ccagcctgcg gcgcaagggg
     6121 ccccgcggca gctccgaaac gggatggccc tcgaagcgga tcacgccccc cgtaggctcg
     6181 tagagccgcg cgatcaggtt caggagcgtc gtcttccccg agccggtccg cccgaccacg
     6241 gcgagcgtct cgccggcgcg gatcgagagg ttcaaggcgt ggagcacgtc gcggtcggtg
     6301 cccggatagt ggaaggagac gttctcgagc gcgaccgcgg gcacgtgggc gtgcgacgcg
     6361 ccgtccggcg cggcacggtc aggacggggc catggagtcc cgggctccac gtcgtcctcc
     6421 accggcagcg ccaggatctc ctggatccgc cccatggagg ccgcgccgcg ctggaacagg
     6481 ttcgacaccc accccagcga gatcatcggc cagatgagca tcgcgaggta gatctggaac
     6541 gcgaccagct cgcccagcgt gatctggccg cgcaccaccg cggcgccgcc cacgcccagg
     6601 acgatcgccg aggcaatccc gcccaggagc gacagcatcg gccacattcc cgaccagatc
     6661 ttgatcatcg cccggttctt ctcgaggtag tcctcgtgga tggcggcgaa cgcgcggtac
     6721 tccccttcct cttccacgtg cgccttcacg acgcgaatcc cggcgagggt ctcctgcgcc
     6781 ttggccgtca tcgcggcgaa cgaggcctgg atcgcctcgt agtggtggtg cagcttggcg
     6841 cccaggcgcg cgaccgtgaa cgagaggatg ggcagcggaa tcagggagag gagcgtgagc
     6901 cggagatcga tccggcacat cagcgtcacc gcgagaacga acgtcgcgat ggtgtttccg
     6961 aagtacatga tgccgggtcc caggaagttg cggaccgcgt ccaggtcgtt ggtcgcgcgc
     7021 gagagcaggt cgcccacctt gcgcgtgcgg taggtctcgg ccggaaggcg cgtgaagtgg
     7081 tcgaacaggt cgttccgcag ctcgtactcc atgcgccgcg acgcccggat gagcgacatg
     7141 cgcatggtga acaggaagac gccctgcagc gtgaccgcga gcaggatcag cccggagtcg
     7201 cgcaggagcg ctgagcgggt gacgccggcc tggagatggt ccacggcctg gcgcaccagc
     7261 cagggcgacc acacctggaa gaccgtggtc aggccgatcg agagcgtgcc gaagaagatc
     7321 gcgatcttgt gccggcgcag gtatggaagc aggtggcgaa gcgatccgat gacggtgatc
     7381 ctcgctggat gtggggaggc gggcccgacg ctcgccgggg cgagccgccc gccggagacg
     7441 ggccgctccg ccgggactag gaggagctgc cctcgttgta gactaagatg cggccacagg
     7501 cctcgcagat catgatcgcg tcgcccaagc gcacttgctg catcgcgtgg ggggtcaggg
     7561 cccggaagca cgccccgcag gcgcccttct tcacctcggc caccgccacc ccatcccgcg
     7621 cccccgccag acgatcgtac cgcgagagca ccggcgcggg cacgcgcgga cgcacggcgt
     7681 cccggtcctg catcagggcc gcgagggtct gctccagcgc cgcggcctcc tggtccatcg
     7741 aagcctcttc ccgcttcagg cgcccttcct cctccgcgac ccgcttctcc gcgctcttca
     7801 tcgcaaggac cagcttctcc tcggcgtcgt agctctcgag gatcttggtc tcgacgtcgg
     7861 agcgcttctg cttcaccccc tcgatctggt gcctcatcgc cgtgtactcc tggttcgtct
     7921 tcacgtccag gagctgccgc tccagcttgg ccacgtcggc ctggaacgcc tccacttcct
     7981 tctccaggga gcggcggccg ccgcggccgg cgtccagctc cttcttggcg cgttccaggt
     8041 cggccctggc cgcggccagg gcgcctcgca gcgactcgcg ccgagacggg atcgcctcct
     8101 tgcggcgttt cgcgtcgagg agaagggtgt cgcggcgctg cagctcgaga agcgcttcca
     8161 gctcagggag cacggcggtc ctttcgcgag gttctgggga aaagaaaacg gggagcgcgc
     8221 acgagcgcgc tccccgtggt cggcgacggg ggccggcgat gagcgccgtg cccccgcgaa
     8281 ggtccggccg gttcgagtcc ggccccaagc cgggccgcgg cggcgaccct ggattccctc
     8341 ggtccaaaat ggtgggcggt gcagggattg aacctgcgac ctctggcttg tgataccagc
     8401 gctctcccaa ctgagctaac cgccccgacg aggaccgagg ccgcgggccc gcgcgatgcg
     8461 tcgcgaggaa tcatggacgg gaaaagggca agtgggccca cttggactcg aaccaaggtc
     8521 cgaccggtta tgagccgggc gctctcacca actgagctat gggcccggtt gcgcttcgat
     8581 ccggcttgga acccgagcgc cggagggtac cagaggcgcg acgggagggt caaggaagcg
     8641 gccgcgccgc ttcccgcccg cgcggcgctt ccgctcacat catctccacg tagcccttga
     8701 gccggcggct gcgcgtcgga tgccgcagct tgcgaagcgc tttcgcctcg atctggcgga
     8761 tgcgctcgcg cgtgacgttg aacgtggcgc ccacttcttc cagcgtgcgc gggcagccgt
     8821 cctcggtcag gccgaagcgg aggcggatga ccttggcctc gcgcttggag agcgtctcca
     8881 gcacctcgtt cacctcgtcc ttcagcatgg agaacgcggc cgcctgcgcg ggcgagacca
     8941 cgctcgtgtc ctcgatgaag tcgcccaggc tggaatcgtc gtcctcgccg atcggacggt
     9001 cgagcgagat cggctcctgc gccgccttga gcacgctctt gatcttgtcc acgggcagcg
     9061 cgagcttcgc cgccacttcc tcgggggtcg gctcgcggcc cagctcctgc accagccggc
     9121 gcgacgtgcg cacgaccttg ttgatcgcct cgatcatgtg caccggaacg cgaatcgtcc
     9181 gcgcctggtc ggcgatggcg cgcgtgatcg cctgccggat ccaccacgtc gcgtaggtgg
     9241 agaacttgta tcccttccgg tagtcgaact tgtccacggc gcgcatcagg ccgctgttcc
     9301 cctcctggat caggtcgagg aattcgaggc cgcggttggt atagcgcttg gcgatggaga
     9361 tcacgaggcg cacgttggcc tcgatcatct cgcgcttcgc cttgtcgcgg gcgatctcgc
     9421 cggagcggat ctcgcgcgcg acctccttca cctggtcgcg cgtcatgtgg gcttcttctt
     9481 cgatcttccg caccttgcgg cgcgcgttct tcgaagcgcg gtcgtactcc tcgaagcgct
     9541 cgggcgtgag ggcgctctcc ttctcgacct gcttcgcctc tttcttgctc ttcttggcgc
     9601 gcttcaggag gtcggcgatc tcgtcgccgt tcttgcccca gcgccgctcg agatcggtga
     9661 actcgctctc gaccaggtcg atctggtgcg agtagtcctt gatccgctcg gcgagccgct
     9721 cgatgaggcg cgtgttcagg ttcagcgtga acagctcgca catgaggtct tcgtccgcgg
     9781 tgcgcagctt cccctggtgg gtggcgcgca gcgcttcgga caggcgtccc gtcggctttt
     9841 cctggagctt ggtgaacttg ccgtgcagga ggcggatctt gtcgaacgcc ttgagggcgc
     9901 gcgcccgctc gcgcttgggg ctgaactcgg gattccagtt gccaaagtcg atctgggtca
     9961 ggtcctcgac ccgcatcttc ccctgcttga gccgcgtgcc gtaggagatg atctccttga
    10021 gaatcggccc cgaacggaac agcgcggtgc agatctgctt ctcgccggcc ttgatccgct
    10081 tcgcgatctc gacttcgccc tcgcggtcca gcagcggcac gcgtcccatc tcgcgcaggt
    10141 acatgcgcac gggatcgtcg tagcggaccg gatgggggag cgccgccgcc ttctcgggcg
    10201 ggcgcttctc ctcgcgccgc ttcagacgga gcttctgctg cgcctcttcg tgcgtgtcga
    10261 agacgtcgac cttcaggtcg ttcagggcga tgtagacctc gtccatgtct tcgaccgagg
    10321 cgctctcgtc caggaaggtc tcgatctggt cgtgaagcag gtagtgctgc ttgccggcga
    10381 gcgacttcaa ctcgaccagg cgatcgccgc ccttctcccg cttcaaccct tcggctttgc
    10441 gtttcatggg cactcgcgtt ctcctctagg aaacggccgg atggctacgg ctgtgtaact
    10501 tagatgccag atcccggcgc tcggccacca ggctccccag cagtcccaaa tccttggcgt
    10561 cgtgggaccg ctccgcctgg cgaatggccc ggtcgagatc ctggatgctg gcgcgaatcc
    10621 gccgctcttc caggcgctcg atcagggctc gcgccccccg gcgcgactcc gaatcggtca
    10681 acggctcttc aggcttggga tggccggtca ggacggcgcg ggccaacccg gacgcgttca
    10741 tgagatccaa gagctccagc tcgcccggca cccgaacctc ggccagccag gcgtccagca
    10801 ggtcccgcaa cccctgatgg cgaaagcagt cggcaccccc cttggcgacg acctcgccca
    10861 gggccccctc ctcggtcaga accagcgccg ccagttcgga ttccacggga tcgggagcga
    10921 tccagcccgg ctctcccggc ccggcggcgg gggctcccgc ctcggggggc tccgcggacg
    10981 ccatgcccgc cttcgccccc ggatcggggg ccggagcggg cgctgccggc ttcttgggtg
    11041 cccgtcctcc cttacgctcc agcgcctcca gcagaacctt ctcttccagg gcgaacgcct
    11101 cggccccccg gcggaccagc atcctccgcc gaatcgggtc ctcggaccgc ccgatcacct
    11161 cgacataccg tcgtacccgc gcctcgaccg aagggttctc cccaccggcc cccagcgagg
    11221 cgcccgccag gaacccggcg gcatcggccg cgtcggccag cagcgcctgg aaggcggacg
    11281 gcccctcctt acggatgaag gtatccggat cctcccccgg cggcaggagc agcacccgga
    11341 ccgccaggcc ggcccgcatc agcggctcga gcgcccggtc ggcggcccgc accccggcgt
    11401 cgtcgccgtc gtagaccagg accgcctcgg cctccagccg ggccaggatc cgcgcctggt
    11461 cgtaggtcag cgcggtccca ctggtcgcca cggcgtttcg gaatccggcg gcgtggagcc
    11521 gcatgacgtc caggtacccc tccgcgatca gcgcctgtct cgtatcgcga atcgccgccc
    11581 gggcctgcgg gagcccgaag aggatgctcc ccttccggta gaccgccgtc tcgggcgagt
    11641 tcaggtactt cggctgatcg gccagcgcga tggcgcgggc gccgaagccc gccgtccgct
    11701 gcgccgactc catcacggga aacgtgatcc ggttgcgaaa ccgatcgtac cggctgccgt
    11761 cggcgcgccg gagcgcgagc cccgcctcct ccagcaccgc cacgggaacg agctttccca
    11821 gggtcgtcac gaggccgtcc caccccgcgg gcgcccagcc gatctggaag gcgtcgagcg
    11881 aggccctgtc gaatccgcgg ccggtcatgt actcgcgcgc ttccgcaccc gcctccgaat
    11941 cgaggctggc gcggaagtag tcgttcgcga gcgcgttcgc ctggtagagc cggtcgcgcg
    12001 cgacgtcgcc gggaccgcgg gccgtcttgg gcagctcgat ccccgcccgc tcggccagca
    12061 tccggagcgc ctcgggaaaa gcgaccttct cggtctcggt gacccaggcg aagaggtcgc
    12121 cgcccttgcc gcagccgaag cagtgccata cctggcggtc gggattgacc gtgaacgaag
    12181 gggtccgctc cgcgtggaaa ggacagagcc ccttccacga gcgccccgcg cggcgaagcg
    12241 cgaccttctc gcccaccagg gcgaccaggt ccaccgcctc gcgcacccgc tctttcgctt
    12301 cgctcgagag cattgcggtc atggctccag cgtcacgatg gtcacgccgg tccctccctc
    12361 gcccatctcg cccaggcgga attccttcac cagctggttg tgcgcgagga aacgcttcat
    12421 gtgctcgcgg aggatccctc tccccttccc gtggatgatg cgcacggtct gctgccctcc
    12481 caccgccgcg tcctcgagaa agcgctcgac ggcgcgcacc gcgtcatcgg cctcgtagcc
    12541 gcgcacatcg acctgggtcc cggtgtcctc caggtccgga gtcgcgatcg ccgcaccacg
    12601 gaccggcgcc tgggcggccg cgtccttctc ctcctcatcc tcggaccgct cgtcgcgctt
    12661 cccgcccggg cgagccgcac ccgcggccga aggctgcatc gagcggagct gcgacaccgg
    12721 cagcaccgcc gtcgccgcgc cgatcttcac gcgcacgcgt ccgtccttgt cgggcaggga
    12781 aagcgccgtc ccctcggccc ggagcgcggg gacgaagatc ttgtctccct cgcgcaccgt
    12841 gcggagcgga gcgccgcgct cggccggcgc cggctgcgcc gcggcgtcct ccgccgcgat
    12901 cgattcttcc atcgccttcg ccagcccgcg cccctgctgc agcgtctcgg cgctcgcctt
    12961 ccgctcggag cggaccttct tccgatagtc gtcgatctgg ctctccagct ggcgcaggag
    13021 ctcgcggcgg gcggcgatct cggcctgctt ccgctccttc cgctccgact ccagccgctc
    13081 gcgcgtggag cgaagctcgc tctcgcgctc gcgcagcttg gcctgcgtct ccgtgagcct
    13141 gcggcgctcg gccacggcct cttcgcgcag gcgccccagc tcttcgatca ggcggttcag
    13201 gtcgcgctcc ccctcgggga gcagcgtccg ggcccgcgcg agcacggccg cgtccatgcc
    13261 gaaccgttcg gccacctgga tggcgcggct tccgccgggc acgccgacga ccaggcggaa
    13321 gagggagcgc agcgtggcct cgtcgaacgc catcgacgcg ttccgcacgc cggccgtctc
    13381 gtgcgcgaag gtcttcaggc tcccgtggtg cgtcgtggcg agcgtcatcg ccccccgctg
    13441 gagcagcgcc tcgagcaccg cctggcccag cgccgcgccc tcggcgggat cggtgcccga
    13501 ccccagctcg tccagcagca cgagactccg atcggtcgcc gcggccagcg cctgcgtcac
    13561 gcgccgcacg tgcgagagga aggtgctcac gtcgtcctgg atcgactgct cgtcaccgat
    13621 gtcgcagacg atgcggtcga accacgcgaa cgtggtgccg tgagcggccg tgaccgggag
    13681 ccccgccatc gccatcagcg agaggagccc gacggtcttc atcgcgacgg tcttcccgcc
    13741 catgttcggg ccggtcacga ggagcaccgg gttctcggcg tccagctcga gatcgagcgg
    13801 caccggcggg cccatctcgc cgcgcacatg ccgcagcacg agaatcggat ggcgcgcgcc
    13861 gcgcaggagc agccggcgct cgtccgcgat caccggaagc accgccccga cctcgatggc
    13921 ccaggacgcc acggcctcgg ccgcgtccag cccggccagc gccgccaggt ccgccatgag
    13981 cgcgccgtga tggcggtgcg cctcgaccga gagggagcgc aagatccgct cgacctcgcg
    14041 ggcttcgtcg ctcgcgcgct cggccagctc gctgttcccc tcggcggctt cgcgcggctc
    14101 gacgaatacg ctcgcgcccg agcccgagtg accgagcacc gctccaggca cggagcgccg
    14161 gttggacgac gggatcgcga tcacgtagcg ctcctcgcgc agcgtgacga agctctccgg
    14221 gcttccggcc gcacggagga tcgactccag cttggaggcg agccgctcgc gcagcgatcg
    14281 gatcgagcgg cggatcttcg agagctcggg cgaagcgtcg tccttcacgg cgcccgaggg
    14341 gtcgatcgcg cgcaccaagt cccggcgcag cccctcggcc gggtctagcc cgcccagaag
    14401 ccgctcgagc cccggggcgt cctcgcggcg cttccggtac caggtgagga tctgccccgc
    14461 ctgttccagg aggaccgcga tctgccagag ctcctcgccg cgcagcgtcg agccctccgt
    14521 ctccagacgc tgaagcacga ggccgaggtc gggcggtgcc gcggtgggcg gcggatcgcc
    14581 ggcctcgtgc gcggcgagaa gatcggcggt ctccgcttgc gcggcgcgcg cgtcttccgg
    14641 gcggcgcatc ggggcgcgcg cgagcacacg ctcgcgtccg gggacggaac gggtccggcc
    14701 cgccagcgcg cgaagcaccg cgggaaattc cagaacgtca tgtgcgtgat cccgcgcgcg
    14761 ctccatttgc atgggctccg cggaaccgtt cggcgccgtg gccgggcgct ggtccaattc
    14821 ggtgtggctt ctttccgggg gttcggggcc ggccggcgtt accggcccag gagacaaacg
    14881 ggaaaccggc tcacaccggt ttcccgcctg gatgttcggt tggacgaccc tattcagccg
    14941 acttcattcg ggccatcatc ttcctcttcg cggcgttgag ctttcgcttc ttccgctcgc
    15001 tcggtttctc gaagtgctga tgtctgcgca ggtcggcgag aattccagct ttctcgcact
    15061 tcttcttgaa ccggcgaagg gcactctcga aggactctcc gtccttgacc ctgatgccgg
    15121 gcatcctatg aatcgcctcc ttcgggacag gttaagggtt ccgaacgggc tcgcccaaga
    15181 cgttgggcaa actgcggcta acgctactga agataccccg atagccctgt tttgtcaact
    15241 cggcccaacc ccgccgtcag catcaggtta cgggtggccc caaggagggc aaggcaggcc
    15301 gtggacgtgg tcaagcggct gggccccaaa gagataggac gaaagcttcg catcgtcaga
    15361 agctccccct cttcgcgcag aaatccgcct ggtggcccaa ctgccaatac ggcatgtggt
    15421 tgcgagattt ggagcatggt ctccacaacc tctgcgcgga gcggctcccc tcccggctcg
    15481 gcgaacaacc ggaatgtttc tacgggaatc tcctgaagcg cctcctcgag acccggcgag
    15541 gagcggaggg ctgggagccg ggtgcggcgc gactgcttga gcgccgaccg ggcgatccgc
    15601 gccagccgct gaaggcgtgc ctccgggagc ggttcccgcc cgctgtggcc ggtcgcgacg
    15661 aagtcgatcg cggtcacgcc caggggtacg gcgagcgaca cgatggtctc gatctgctgg
    15721 agatcgggaa ggccggccag gagcgtgatc gccgccccga gctcgcccgc ctccacggct
    15781 cgcgactcga tcgtcccgac cgcgatcccg ccggaagccg tctcgaggac acagcggtag
    15841 agggttcctt cgccgtccac cgcctcgaac ggggtgcccg gcgcggcgcg aaagacgcgg
    15901 aggagatgcc gagactcgga gtcgctcagg cgaagccgcg tcccgtcgac gttggcgcgg
    15961 tccacccaga acgccgtgtc gggcgtccgc gccgcgcctt tcatccggcg gcgctcagcc
    16021 ttcgtcgccc gtccacgccg ggcggcgcgg cgcgggggcc ttggcctcag ggagcgtctc
    16081 gaattcttga agcgctttct tgccgcgctc ggagagcttg gatggaacgg ccaccacgac
    16141 gcgcacgaga aggtcgcccg tccccgagcg ctgaaggctc ttcagcccct tcccgcgcag
    16201 gcggaggagc ttccccgttc ccgtcccggc ggggatctcc accagcgcgc ggccgccgcc
    16261 cagcgtgggc acctcgacct tcccgcccag cgccgcccgc gagaggctga tcggaacctc
    16321 gaccaccaga tcgtcgccgt cgcgcgcgaa cagcgggtgc tcgatctcct cgatgtgggc
    16381 gatcaggtct cccgcgggac cgccgcgcgg tcccacgtcg cccatgccgg gcagcgggat
    16441 gaagttgccg ctcgagacgc cggccggcac cttgatcgcg atctcgctct gctcccgcac
    16501 gcgcccctcg ccgtgacaga ccgggcaggg attctcgacg atggttccct cgccgtggca
    16561 gcggtcgcag gtggagacgt tgatgagctg gccgaagatg gagcgctgca cctggcggat
    16621 ctgccccgag ccgtggcatt tcggacaggt gatccggctg gtcccttcct tcgcgccggt
    16681 ccccttgcag gtcttgcaga cgcggagatg ccgcaccttg acccgctttt cggtgccggt
    16741 cgcgatctcc tcgagggtga gcgggaggcg aagctccagc gtgttgcctc ggcgctcgcg
    16801 gggacggcgg cccacgccgc cgccttcctc gaacaggtcg ccgaatccgc cgggcccgcc
    16861 gaagtcgcgc atgaaggcgc gcagcgcgtc ggccaggtcg aagccgcgca tgtcctcgaa
    16921 accgccgcct ccgccgccgg gaaatccctg cggggcgtgg ccgaagcggt cgtagcgggc
    16981 gcgcttctcc gggtcggaga gaacctcgta ggcctcggcc acttccttga agcgctcctc
    17041 ggcctgcttg tcccccggat tcttgtcggg atggaatttc agcgcgagct gccggtacgc
    17101 cttcttgatc tcctcaggcg acgcgctccg ctccacgccg agcacttcgt agtagtcgcg
    17161 aaccgtgctc acgcgcgatg ccggtcctta ccggacggct cggtccggtc gacgtcggtg
    17221 gcgcccatgc gatcgcgatc ttcgagtgcg ggtggaatcg tgtcgggtgg cgcgctctcg
    17281 cccagcatcg agggtggtgt cgtcgtgtcg gccggcggcc ccgcgacgac caccttggcg
    17341 tggcgcagga cacgatcgtt cagcatgtag cccggctggg ccacgtcgag caccacgccc
    17401 ggccgggttc ccgcgggcgc gacacgctca ccgatcgcgt cgtgcagatt gggatcgaac
    17461 ggctgccccg cggactggat cggggccaag ccctccttct cgaggatctt catcatgcgg
    17521 tcgcgcacca actccacgcc ggcgtgaagc ggatcgtgcg gcgtgccggg catcgccgcg
    17581 agcgcgcgct cgaagtcgtc gagcaccggc aaaagctcgc ggatcaagga ttcctgcgcg
    17641 tagcggaccg tctgctcgga ctccagcttc cggcgcttct gcacgttggt catctcggcg
    17701 agcgcgcgga gaagctcctc ctcgcgatcg cggaggcgct tgatctcggc ctccaccgcg
    17761 tccggatcgc gcccgagggc tccggtatcg ggagcggcgt tcgtgtccgg ggcgtcggcc
    17821 cgggtttcgt ctcggtcttc cgtcgtggct gcgcgtttcg gtttcatgtc tatgctcccg
    17881 cgaggcgggc gcccacgtag gagacgaggg agatcacgcg ccggtacggc atccgcaccg
    17941 gtcccatgac tccgatcgcg ccccgcaggc cgccgaccgc cacgcccatt ccaacgatcg
    18001 agcacgcgcg aatcgcggga gattcgatct cccggccgat cgccacgcgc ggcacgctct
    18061 cgcccgagaa cgcgtccagc gtccggacga gcgactcctg gttcgccagc aaggatacga
    18121 gcggccggag cgttccgggc gcctgaaact cgggctgcga cgcgatgttg tcggcgccgt
    18181 ccacgcggac ggcgggctcc tcgcgcgtcg agaggagccg cgaggcgcgg gtgagcgcct
    18241 cctcgatgaa gctcgcgatc tccaagggtg cgcccgcgcg cgcctccagc accagcgcgt
    18301 ccagcgcctt cggccccgcc gcgggaagcc gcgcgcggat ccaggactcg gcccggcgaa
    18361 ggtccgcggc gcacgggaag agctcgggcc gccacgtatc cgtggccacc atgccgtcgc
    18421 gtgcgcccac ggcgacgacg acgtgcccac cgtccagggc cacgaggttc acctggtcca
    18481 ccgcctcctc ccgcgaccgc accgccatca cggccagctg ccgcgtggcg cgcgcgagga
    18541 tcggcgcgag gtcggaggcg cgcgactgcg tgcgcgcgcg gccgatcgct tcatcgagga
    18601 tgcgtcgcac gccgatcggg accgtctcgg ggcgcatcag atgatcgacg taatagcggt
    18661 agccggcctc cgtagggacg cgtcccgccg acgtgtgggg atgcagcagc agccccagat
    18721 cctccagctc gcgcatggcg ttccggatcg tggcgggcga gatgctcagg tggtaggcgg
    18781 cgcggacgta ctgggagccc accggctcgg cgcgcgagac gtggctttcc accacgaagg
    18841 tcaggatgcg gctgagccgc ggatccaggc gcggatcccg gctatggggg ctatgggggc
    18901 tatgggggat attgggcttc atcggggctc tctcgggacg tttcccggaa ttccgggcgt
    18961 tagcactttc ggctgtagag tgccaacgcg acgacgctag cagcgaggct gtgggctgtc
    19021 aacccggcgg ccctgcgccc gccctcgcag ccgccctagc ccccgccctc gcccccgccc
    19081 tagcaaagag ctccgctgcc gactcgagcg cggtcagaag cccccgctcc gtcggacgaa
    19141 cggtcccgcg cccggatcgc agcgcaccgg ccgcttcgcc ccagcggagc catccttcgg
    19201 cctcgtacgg cgcggcccgg tccagcgccg cccggcgcag cccgcgcgcg cggcgaagtc
    19261 ccaggaacac gacctcctcg cgggcatccg cctcgctcag ggcgcgcacg tcgtgtcgcg
    19321 gcagcgcccc cgcctcgacg cggtcgaacc accgcgccac gtcctcctcg tttccccagc
    19381 gacgcgaccc gtcgaacgaa tgggcgcccg ggcccagccc gagatagggg cggcggatcc
    19441 agtaggactg gttgtggcgc gacgcgaaac cgggcagcgc ccagctcgag acttcgtagc
    19501 gctggtagcc cgcggccgag agcagccgtt ccgcgacgtc gtagcgttcg gctgcctcgt
    19561 cctccgccgg gatcgcgatc gccccgcgcg cggccttggc cgccagcggc gtcccctcct
    19621 ccacctggag cgaatagacc gagaggtgcg gcacgctcag cgcgatgacg cgctcgacgc
    19681 cgcgtgcgaa gccatccgcg gtttccccgg gaacgccggc catcaggtcc agggatacgt
    19741 tcgtgaagcc cgcggcgcgc gcgcgcgcga gcgcgtccag ggcgccctgg gcggtgtgga
    19801 tcctcccaag cgcgcggagc gcttcggcct cgaaggactg gacgccgagg ctcagacggt
    19861 ccacgccggc ccgccgcagc agacgcagct tctccgccgt caatccctcg ggattcgcct
    19921 ccaccgtcca ctcgcggatg cgcgcggccg gaaacgccga acggacccag tcgaggaagg
    19981 ccgcgagggt cttctcgtcg agcgcggtcg gcgttccgcc gccgacgaag atcgtccggg
    20041 gacggttctc gacccacggc cccggagcct cggccgcggc cagctcccac tcccgcgcca
    20101 gcgcgcgcag gagccgctcc ggctgcgcgt ggtggagcgg cgccttggag aaggcgcaat
    20161 aggggcacag gctccggcag tagggcagat ggacgtagag tccgtagtcc atcacgccgt
    20221 ccgcaaggcg cgttgggaca tgaatcgcct cagtggatcg gccggaagag ccggttcagc
    20281 cgcggcagtc tccggtcctg gtcccacgac cagtagatga acatcgcctt tcccttgacc
    20341 agctcgcgcg gcaaggcgcc ccagaagcgg ctgtcctgac tgttgtcgcg gttgtcgccc
    20401 atcatgaaga gatgcccctg cggcacgacg taggggccgt agttgtcgcg cggatacgtc
    20461 tcgcgcggga tgatgcgggg gtccttgtgc acgacgtagg gctccacggg cgccttgccg
    20521 tccacgtaca gcaccttgtc gcggatctcg acggtctgtc ccgcgacggc cacgcagcgc
    20581 ttgatgaagt cccgcgaggg gtcctccgga tatcggaaga cgaccacgtc ccccggcttc
    20641 ggctcgcgga tcgtcgggaa gcgatagtag aagaagcgct cgccgccgaa gccgatgtcg
    20701 atctccgagc cgtagaccat cttgttcacg aagaggaagt cgccgacctg cagcgtgtcc
    20761 tccatcgagc ccgtcggaat gcggaacgcc tggaggacca gcccgcggat gacgatcgtg
    20821 aggacgatcg ccaggaggat cgcttcgagg tattcgcgaa gggccgactt cgtgcgggcc
    20881 cgcgcgcgcg cggccgccac ggaggatccg ttggtcatgg tctcctttcg cgccatgaac
    20941 cggcgctagc gttccacccg gagcaccgcc aggaacgctt cctgggggat gcggaccgtg
    21001 cccacctgct tcatccgctg cttcccttcc ttctgcttct cgaggagctt tcgcttgcgg
    21061 ctgatgtcgc cgccgtagca cttggcgatg acgttcttcc ggagcgccga gatcgtctcg
    21121 cgcgcgatca cctggccgcc gatggccgcc tggatcgcga tcgggaacat ctggcgcgga
    21181 atcagctcct tgagcttcac gctgatgtcg cgtccccaat cgtacgcttt ctcacggtgg
    21241 acgatcaccg acagcgcgtc caccggatcc ccgttcaaga gcacgtccat cttcaccagc
    21301 tcggccggct tgtaccccgc caactcgtag tcgagcgagg cgtagccgcg gctgatcgat
    21361 ttcaggcggt cgtagaagtc gagga
//
LOCUS       DASSLZ010000281         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_989788, whole genome shotgun sequence.
ACCESSION   DASSLZ010000281 DASSLZ010000000
VERSION     DASSLZ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989788"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="VFM00_10720"
     CDS             complement(<1..1237)
                     /locus_tag="VFM00_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498762.1"
                     /translation="MSAAEQEKGLVVDTRELVWKAKRYKWLALLPIVGALCVAFLYLN
                     VAPSVYESSVVISMEDPTRVSEGMERLVRPSEQQEDLVQKMARVRSRVLNRTFLTAIS
                     DRLALGRDPKLVSISTAAARKYPGITPEEYATRIAVSTLAKRINVLPVGSTYIRLAVK
                     DPEPEQARRLATAVGEGLIEETRKSELERTQARGEFSQDQIAVERENLRRASDAYRAA
                     QERVVGSTITSGSVDPGNVEAIRELADAADKEADQVSERIRSDKQTWVDRMGSARMPD
                     LRTSRTSELETRLSGLEASYGMAAARGSSQGDASGQSLLQQIGAARQELLSEYENAAG
                     SLQGNIPDDAKQIAAGVALDRSILTSLRARKGRLNSMVGSYVSRVRSAPASQMELDGL
                     KAEMQKHQDLLATLEKEAAS"
     gene            complement(1245..2261)
                     /locus_tag="VFM00_10725"
     CDS             complement(1245..2261)
                     /locus_tag="VFM00_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET7498763.1"
                     /translation="MTGICRFLQNSSTGRKILGRTWHTSALGLAIFGLLSAVLAAPGW
                     AQSTRPPAPSGTQEEAYRVGKGDVIRVDVPGHTDMSGSFTVADDGTVSVPNLGTIRVQ
                     GRTANEIRSDLGRRVSLIDRSSPPVSVTVAEFKSRKIFVLGAVLLPGIYAFAEMPTIW
                     DAIAEAGGPLDDANLNAVELIPGDAGGGRKTETIDIASAIRESRTETLPRLRPGDTVR
                     VPRGQNATSVLLTGAVLRPGPLPYDQAPDLVTAVVRSGGVAADADQNQVQVIRQSGER
                     SMRIRVNLNKYFEKGDASSNPHLEPGDTVFIPRKEGTSIWVHLAQMSSVISLVTSIIA
                     ISRH"
     gene            complement(2353..>3108)
                     /locus_tag="VFM00_10730"
     CDS             complement(2353..>3108)
                     /locus_tag="VFM00_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HET7498764.1"
                     /translation="EELNRIVTDALADILFATSADAVENLEREGIEAAKIHLVGNSMI
                     DSLRRHLPAARARRAPERFGLERGRYAVATIHRPSNVDDPAQLARVLEALRTLSSSLP
                     VILPVHPRTAARIAAMEEAGAALHAAPGLSIVPPLGYLDFLGLMDAARLVLTDSGGVQ
                     EETTALGVRCITLRDTTERPITMTEGTNRLVGSDPAAILPAAREALAAGPVEARMPAL
                     WDGRAGERIAAILARQPGLGAGLRREKTATATV"
BASE COUNT          436 a         1054 c         1084 g          534 t
ORIGIN      
        1 acgacgccgc ttccttctcc agcgtggcga gcaggtcttg gtgtttctgc atctcggcct
       61 tgagcccgtc cagctccatc tgcgaggccg gcgcgcttcg aacgcggctg acgtacgagc
      121 cgaccatgga gttgagccgt cccttgcggg cgcggagcga cgtgaggatc gagcggtcga
      181 gcgcgacccc ggcggcgatc tgcttggcgt cgtccgggat gtttccctgg agcgaccccg
      241 cggcgttctc gtactcggag aggagctcct ggcgggcggc accgatctgc tggaggagcg
      301 actggccgga ggcgtcgccc tgcgaggagc cgcgggccgc ggccatgccg tagctggctt
      361 cgaggccgga gagccgtgtt tcgagctccg aggtgcggct ggtgcggagg tcgggcatcc
      421 gcgccgatcc catccggtcg acccacgtct gcttgtcgct ccggatccgc tcggagacct
      481 ggtcggcttc cttgtccgcg gcgtcggcca gctcgcggat cgcctcgacg ttgccggggt
      541 ccaccgatcc ggacgtgatg gtcgatccca ccacccgctc ctgggcggcg cggtaggcgt
      601 ccgaagcccg gcgcaggttt tcacgctcca ccgcgatctg atcctggctg aactccccgc
      661 gggcctgggt ccgttccagc tcgctcttcc gggtctcctc gatcaggccc tcgcccaccg
      721 cggtcgccag acggcgcgcc tgttcaggct cggggtcctt cacggccagc cggatgtagg
      781 tcgagccgac cgggaggacg ttgatccgct tggccagcgt ggagacggcg atgcgggtcg
      841 cgtactcctc gggggtgatg ccggggtact tccgggccgc ggcggtggag atcgacacca
      901 gcttggggtc gcgcccgagt gccaggcggt cgctgatggc cgtgaggaat gtgcggttca
      961 agacgcggct tcggacccgc gccatcttct ggacgaggtc ttcctgctgt tcgctggggc
     1021 gcaccaggcg ctccatccct tccgaaaccc gcgtcgggtc ctccatcgag atcacgacgg
     1081 aggactcgta gacgctgggc gccacgttca ggtagaggaa cgccacgcag agcgcgccga
     1141 cgatcgggag gagggcgagc cacttgtatc gcttcgcctt ccacacgagc tcgcgcgtat
     1201 cgacgacgag tcccttttcc tgctcggctg cgctcatccg cgactcaatg cctggagatg
     1261 gcgatgatcg acgtgaccag ggagatcacg gagctcatct gtgccaggtg tacccagatc
     1321 gaggtgcctt ccttccgtgg gatgaaaacg gtatcgcccg gctccaggtg cggattggac
     1381 gaagcatcgc ccttctcgaa gtacttgttg aggttgacgc gaatccgcat ggaacgctcg
     1441 cccgactgac ggatgacctg cacctgattc tggtccgcgt cggcggccac gccgccgctg
     1501 cgcaccacgg ccgtgacgag gtccggagcc tgatcgtacg gcagcgggcc gggccgcagg
     1561 accgcgccgg tcaggaggac gctggtcgcg ttctggcccc ggggcacgcg cacggtatcc
     1621 cccggccgga gacgcggcag cgtttcggtc cggctctcgc ggatggcgct cgcgatgtcg
     1681 atcgtctcgg tcttccgtcc gccgcccgcg tcaccgggga tgagctccac ggcgttcagg
     1741 ttggcgtcgt ccagagggcc gccggcttcg gcgatggcgt cccatatcgt cggcatctcc
     1801 gcgaacgcgt agatgcccgg caggagcacg gcgcccagga cgaagatctt ccggctcttg
     1861 aactcggcca ccgtcacgct gaccgggggc gagcttcggt cgatcaagga gacgcggcgc
     1921 ccgaggtcgc tgcggatctc gttggcggtc cttccctgga cgcggatcgt acccaagttg
     1981 gggacgctga cggttccatc gtcggcgacg gtgaacgacc cgctcatgtc ggtatggccc
     2041 ggcacgtcga cgcggatgac gtcccctttg cccacgcgat aggcctcttc ctgcgttccc
     2101 gagggcgcgg gcgggcgtgt gctctgggcc catcccggcg cggcgaggac ggccgacagc
     2161 agtccaaaga tcgcaagccc cagggcggac gtgtgccatg tcctgcccag gatcttacgc
     2221 ccggtactcg aattttgtag gaaacggcaa atgccggtca tggggtccct ttcgatcggc
     2281 ggcgcagcgg ccaggttcaa aacaggcaac tgcagtccag ccttagattt taacgtgtta
     2341 ccggccccac tgtcaaaccg tggccgtggc cgttttctcc cggcggaggc cggcacccaa
     2401 gcccggctgc cgcgcgagga tggcggcgat gcgctctccg gcgcggccgt cccagagcgc
     2461 gggcatgcgg gcttcgacag gtccggccgc gagcgcttcc ctcgcggcgg gcagaatggc
     2521 cgccgggtcg cttccaacga gccggttggt gccctcggtc atggtgatcg ggcgctcggt
     2581 ggtgtcgcgc agcgtgatac agcgtacccc aagcgcggtg gtttcctcct ggacgccccc
     2641 gctgtccgtc agcacgaggc gtgccgcatc catcagcccg agaaagtcca gatagccgag
     2701 cggcggcacg atcgagagtc ccggcgcggc atgaagcgcc gctcccgcct cttccatcgc
     2761 cgcgatccgc gccgccgtgc gcggatggac gggcaggatc acgggaagcg acgaggacag
     2821 cgtccggagc gcctccagca cgcgcgccag ctgtgcgggg tcgtccacgt tgctcggccg
     2881 gtggatcgtc gcgaccgcgt acctgccgcg ctccaggccg aagcgttccg gcgcccggcg
     2941 cgcccgcgcc gccggaagat ggcggcgcag cgaatcgatc atcgagtttc ccacgagatg
     3001 gatcttcgcc gcctcgatcc cctcgcgctc gaggttttcg accgcgtcgg ccgacgtggc
     3061 gaagaggatg tcggcgagcg cgtcggtgac gatgcggttc agctcctc
//
LOCUS       DASSLZ010000282        11213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_860726, whole genome shotgun sequence.
ACCESSION   DASSLZ010000282 DASSLZ010000000
VERSION     DASSLZ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11213
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860726"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFM00_10735"
     CDS             complement(<1..557)
                     /locus_tag="VFM00_10735"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7498765.1"
                     /translation="MAQAAAVRMREPAARTRSPRIRAKRERRREEILHAALRAFRHRG
                     YHRTTLDDIARRLGVRKTALYHYFPDKEAILYECHKDSLAELDRILAETRRLPTARER
                     LGHVIREHVRVMTDTLEGSPLAFEVTAFSRGPRAEIIAARDRYERELRRILAAGIRAG
                     AFRKTDPKVAAFAVLGAINWIARWYR"
     gene            complement(706..3369)
                     /locus_tag="VFM00_10740"
     CDS             complement(706..3369)
                     /locus_tag="VFM00_10740"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HET7498766.1"
                     /translation="MIALILCALLLLAPAAGAEPPDSAHAYVHANAPVIQAAPLRDNI
                     RVDGKLDEAAWAAATPVDQFTQRDPEEGKPVSERTEVRILIGDDALYVGAHLFDREPS
                     KIRRRLVRRDEDLASDFFAVLIDSYHDHLTAFRFRVNPAGSFDDATIDARGNADFSWD
                     PVWHVHTIIDSTGWTAEMEIPLSQLRFNPSADGVWGIQIRRWIDRKQELAEFAFTPKK
                     EQSDVSRYGHLSGLGALHARRQIELLPYGLAKAQNTFAPPGDPFHDGSEQSGSFGGDL
                     KWGIKDDLTLNATVHPDFGQVEVDPAVVNLSATETFFPEKRPFFVERSELFNFGQTQS
                     SNYFSVPTTFHARRIGRAPQRSLSPFDYSYLDTPSLTTIAAAAKLTGKTRSGWSIGAL
                     DAVTTEERARYLDTLGMARRLPVEPLTNYAVARVRRELRGGNTRIGAIVTDVSRDLSD
                     PALESMLRSRATVAGIDFMHYWAGRRWSLDGYVQGSRVEGSAEAIAATQRSSAHYFQR
                     PDADHVEFDPTRTHLTGMSDLISLNKLGGKHWKGNVTYQDLTPGFESNDMGYISNVDT
                     WGVSTLVMYKEDKPSPLFRNWYAMAFSNNAWNHGGNLQYQGYEAQGSGTFANYWYGDL
                     RGSWYPKAYDDRLTRGGPLTTIPSGGRVAATVNTDSRTSYFFSLHSDFSWNDAGANAQ
                     RYSPSLTLHPAPSLLVRVEPSIQKIRDMAQYVTTVPDPNATATYGSRYVFATLDEHVV
                     SLDTRVDWTFSPRLSFQLYLQPYVVSALFRELKELRAPSTYDFDVYGGSRGTITRDPS
                     GLYTIDPDGPGPSSSFALGDPNFNFRSLLGNAVLRWEYRPGSVLFLVWQQNRNGAQPF
                     GDFDFSRDVRAIFDESPENIVAIKATYWLGL"
     gene            3573..4706
                     /locus_tag="VFM00_10745"
     CDS             3573..4706
                     /locus_tag="VFM00_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7498767.1"
                     /translation="MARQPARRYRFIVSLTFLIPMLVCLAAPPVLAAPEQGERGRSPA
                     SVVTRERSVTEVGAGIYVIRHPDGADTNPQGNTTVVIGSRDVLVVDSGYLPSSARDDI
                     AQIRRWTKKPVRYLVNTHWHPDHVRGNAAYAAAFPGLAIIAHETTPELERGFDEPNLA
                     RYRARVETLRHELSTGRAPDGKPLSDTERTEAGADLARRLPVLREFEGYEPVYPSVTF
                     ADAMTLDLGGRTVQLRHPGRGHTVGDVIAWLPRERVLIAGDLVTHPVPYFFAGWPYDE
                     IDTLERLAALDARVIVPGHGQVLHDRRYLDQTLELLRSVRAQVVAEVRKRGSLSAKLE
                     DVRRAIDFGAAERRFAGSDSESVEFFRESMDGLVKTFFYQIAE"
     gene            4807..5322
                     /locus_tag="VFM00_10750"
     CDS             4807..5322
                     /locus_tag="VFM00_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498768.1"
                     /translation="MRRAPLPHGPRTPILVFRAALALAAALVLATAAAGRATAAPPCA
                     RTAGQSAWAFLWTPTWLPGVPQAAGTALQGDAATAGLWIALDPVTRRPVRPTPEQRRD
                     AGLAAPEVDEVLPVERIPGGGELLHLNGRHQVFEVARRDATGRFRTSCASDSATATRV
                     LATPVSAREAK"
     gene            5319..7013
                     /locus_tag="VFM00_10755"
     CDS             5319..7013
                     /locus_tag="VFM00_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HET7498769.1"
                     /translation="MIRALHAVTLGVAAAILLLAAPFAEAGSFVLINLDGAGEGFNDP
                     TPVSPIGGNPGTTLGQQRLNVFQTAGKIWGAYIPDNVTIRVEARFDPLTPCDSVSGVL
                     GSAGAITLASDFAGAPLASTWYPIALANKLAGTDLATTVNDITAHFNSSVDNSTCLGT
                     SGWYYGYDHNEGTNIDLLAVVLHELSHGLGFQTFTNLSTGGFQNGKPDVYARHLFDRT
                     LNEHWDQMTSNQRSNSAVNTGNLVWDGSFVTNAAHLFLGPAAIVRIDSPAVIAGEKDY
                     GTADFGAPPPNPPLQGQVVLVNDGVGTTSDACEPIVNGAQIAGKIALIDRGTCTFVAK
                     AAAAQAAGAIAVIIGNNVAGAPPGMTGVDPSVTIPVVSITQADATAIKGQLGVGVFAT
                     IGIDAAHLAGTDTQGRVKLYAPNPLESGSNVSHFDVSATPNLLEEPFINSDLTDSPDL
                     NQYLFADIGWWTGAAAVATAQAAGAPRAFSAPNPFSAGTAIRFSLARSGKTDVAVFDA
                     RGALVKRLASSWRPAGAQRVEWDGTDASGGRAPAGVYYWRVTSEEAPQTGRVVRIP"
     gene            complement(7117..7590)
                     /locus_tag="VFM00_10760"
     CDS             complement(7117..7590)
                     /locus_tag="VFM00_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7498770.1"
                     /translation="MKRSLFGWAAACLVLGAALTMLPSLASAAPRDRAEEVRASERAF
                     AKTMADRDLKAFGSYIAVEGVFFGRNGATRGRKAVVEAWTKFFDGPDAPFSWEPETIE
                     VLKSGKLALSSGPVKDPKGALIGTFSTIWRLESDGKWRVVFDKGCPVCEPEKSGS"
     gene            complement(8115..10646)
                     /locus_tag="VFM00_10765"
     CDS             complement(8115..10646)
                     /locus_tag="VFM00_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7498771.1"
                     /translation="MNADDPLHDSLDELASSVSDGAPIDWDAARTDALSPETDASFRA
                     LQDLARIAAFNRALQRGPEGDGADAEAEAGTGVEQTGGADEPGRWGHFTLLEPIGAGA
                     AGEVWRAWDASLHREVAVKFLQARAAAPGADGASTAPGAGPSAALLGEARALARVRHP
                     SVVAVYGIAELDGRPGMWMELLRGRALASEIETRGALSPAETARIGAQLCAALEAIDQ
                     AGLVHRDIKPANVVLESDGRVVLTDFGLGWRRRLIPADAPRKGSGTPLFMSPDLLDGG
                     PPTPRTDLYALGVTLRWALTGHPPFHARTLEELRQEALRGPARPLSSERPDAPAGLIA
                     AIERAMSSGPERPGPFTASAMRALLAPIADEEPDGQRRGARSRSRRASVAVLPFLNRS
                     DEADEYFSDGLADEMISVLSTIRGLHVAARTSAFQFKGKSEDLAVIARKLGVETVLEG
                     SVRKAGDRVRISVRLVRASDGDQLWAETFDRRLDDIFAVQDDIAQCVVQELRAALLPG
                     GAPGAALEAIRAEVADAVRGRGRDPEAHRLYLQGRFFANRKTPEDLTRAIGLLREAVA
                     RDPSDALAWAELGNAHADEAYFGMRSNEDGYARAREAVGRALTLEPDLASAHARMARI
                     QFVHDRDWARAEASFLRALDRDPNNATALHGAAILLQALGRSDEAVRMNLRAIALDPL
                     SAASQNNLGCVYYESGRLAEAEAALRMALELSPNRYGTRSFLAQVLMEEGRGEEALEE
                     AVREPDDVYRLLTLAIIHHRQDREAESLEAIRELAARHGEEFGLQVAEAHGACGHADE
                     AFAWLERAFERRDSGLMHIKPSPHFRSVRSDPRWGAFLKKLGLED"
     gene            complement(10636..>11213)
                     /locus_tag="VFM00_10770"
     CDS             complement(10636..>11213)
                     /locus_tag="VFM00_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7498772.1"
                     /translation="DSPAPELVITGELLHRAKHGDEAALNALMARYRPRLQRWATGRL
                     PRYARSLFDTGDLVQETLLKAIEGLDLIEVRGPGVFQAYVRQAVLNRIKDQVRWARRR
                     PGPDGVPEDLIDVSPSPLESAIGSDVLRRFEGALARLTDDERQLLHLRIELDFDYEEI
                     AAVTERATRDAARMAVQRALRKLADIMGHER"
BASE COUNT         1702 a         3895 c         3935 g         1681 t
ORIGIN      
        1 cggtaccagc gcgcgatcca gttgatcgcg ccgagcacgg cgaacgcggc gaccttggga
       61 tcggtcttcc ggaacgctcc ggcgcggatg ccggcggcca ggatccgccg gagctcgcgc
      121 tcgtagcggt cgcgggccgc gatgatctcc gcccgcggcc cccgggagaa cgcggtgacc
      181 tcgaacgcga gcggcgatcc ctcgagcgtg tcggtcatga cgcgcacgtg ctcgcggatc
      241 acgtgcccga gacgctcgcg cgcggtcggg agccgccggg tctcggccag gatccggtcc
      301 agctcggcga gcgagtcctt gtggcactcg tagaggatcg cttccttgtc ggggaaatag
      361 tggtagagcg cggtcttccg cacgcccagg cggcgcgcga tgtcgtcgag ggtggtccgg
      421 tggtagccgc gatggcggaa ggcccggagt gccgcgtgga ggatctcctc gcggcggcgc
      481 tcgcgcttgg cgcgaatgcg cggggagcgc gttcgcgcgg cgggctcgcg catgcgtacg
      541 gcggcggctt gggccatcgg tcacctcccg ggaaaatttg aatcgatggt cgaagattga
      601 atccgggttc agcgtctgtc acctgacaag gtgtcaggga taccaaagga ggatgcgggg
      661 cttcggcgac cgcttcgcga gggcgcggcg gaggatcgag cgcggctaca gaccgagcca
      721 gtacgtcgcc ttgatcgcga cgatgttctc cggggattcg tcgaagatgg cgcgcacgtc
      781 gcgcgagaag tcgaagtcgc cgaagggctg cgcgccgttt cggttctgct gccacaccag
      841 gaagaggacc gacccgggac ggtactccca gcggagcacg gcgttgccga gcagcgatcg
      901 gaagttgaag ttgggatcgc cgagggcgaa cgacgacgag ggccccgggc cgtcgggatc
      961 gatggtgtag aggccggagg ggtcccgcgt gatggtgccg cggcttcctc cgtagacgtc
     1021 gaagtcgtac gtgctcgggg cccgcaattc cttgagctcc cggaacaggg cggagaccac
     1081 gtagggctgc aggtagagct ggaacgagag ccgcggcgag aacgtccagt ccacgcgcgt
     1141 gtccaggctg accacgtgtt cgtcgagcgt cgcgaagacg tagcggctcc cgtacgtggc
     1201 ggtcgcgttc ggatcgggca cggtcgtcac gtactgggcc atgtcgcgga tcttctggat
     1261 gctgggctcg acgcgcacca gcagggacgg cgccggatgc agcgtcaagg acggcgagta
     1321 cctctgggcg ttggcaccgg cgtcgttcca tgagaaatcg gagtgcaggc tgaagaagta
     1381 gctcgtgcgc gagtcggtgt tcacggtggc cgcgacgcgc ccgccggaag ggatcgtcgt
     1441 cagcggcccc ccgcgcgtca gccggtcgtc gtacgccttc ggataccacg atccccgcag
     1501 gtcgccgtac cagtagttcg cgaacgtgcc cgagccctgc gcctcgtagc cctggtactg
     1561 gagattcccc ccgtgattcc aggcgttgtt gctgaaggcc atcgcgtacc agttccggaa
     1621 cagcgggctc ggcttgtcct ccttgtacat cacgagcgtc gagacgcccc aggtatccac
     1681 gttgctgatg tagcccatgt cgttgctctc gaacccgggc gtcaggtcct ggtacgtgac
     1741 gttgcccttc cagtgcttcc cgcccagctt gttcagcgag atcaggtccg acatccccgt
     1801 gagatgcgtc cgggtcggat cgaactccac gtggtcggcg tcgggacgct ggaagtagtg
     1861 cgcgctggac cgctgggtgg ccgcgatcgc ttcggcgctc ccctcgacgc ggctcccctg
     1921 cacgtagccg tccagggacc accgccgtcc cgcccagtag tgcatgaagt cgatccccgc
     1981 caccgtggcg cgcgaccgga gcatcgattc cagggccgga tcggacaggt cgcgggagac
     2041 gtcggtgacg atcgcgccga tccgggtgtt ccccccgcgc agctcccggc ggacgcgcgc
     2101 gacggcatag ttcgtcagcg gctccacggg gagccggcgc gccatgccca gcgtgtccag
     2161 atagcgcgcc cgctcctcgg tggtcaccgc gtccagggcg ccgatcgacc agccgctgcg
     2221 cgtcttgccg gtcagcttgg ccgcggcggc gatcgtggtg agcgaggggg tgtccagata
     2281 ggagtagtca aacggggaca gcgaccgctg cggcgcgcgc ccgatgcggc gcgcgtggaa
     2341 cgtggtcggc acgctgaaat agttggagct ctgcgtctgg ccgaagttga acagctccga
     2401 gcgttcgacg aagaacggcc gcttctcagg aaagaaggtc tccgtggccg acaggttcac
     2461 gaccgccgga tccacctcga cctggccgaa gtcgggatgg accgtcgcgt tcagcgtcag
     2521 gtcgtccttg atcccccact tgagatcccc cccgaacgag ccgctctgct ccgagccgtc
     2581 atggaaggga tcgcccggag gcgcgaaggt gttctgcgcc ttggccaggc catagggcaa
     2641 gagctcgatc tggcgccgcg cgtgcaacgc gcccagcccc gacagatggc cgtagcggct
     2701 cacgtcggac tgctccttct tcggcgtgaa ggcgaattcg gcgagctcct gcttgcgatc
     2761 gatccaccgc cggatctgaa tcccccagac gccgtcggcg gaaggattga agcggagctg
     2821 ggagaggggg atctccatct ccgccgtcca tcccgtcgag tcgatgatcg tgtgcacgtg
     2881 ccagacggga tcccaggaga agtcggcgtt tccgcgcgcg tcgatcgtgg cgtcgtcgaa
     2941 ggaacccgcg gggttgaccc ggaaccggaa ggccgtcagg tggtcgtggt aggagtcgat
     3001 gagcacggcg aagaaatcgg aggcgagatc ttcatcgcgg cgcacaagcc gccggcggat
     3061 cttcgagggc tcgcggtcga agaggtgcgc gccgacgtag agcgcgtcgt cgccgatcag
     3121 gatccgcacc tcggtccgtt cggaaacggg cttcccttcc tcgggatcgc gctgggtgaa
     3181 ctggtcgacg ggcgtggcgg cggcccaggc cgcctcgtcc agcttgccgt cgacgcggat
     3241 gttgtcgcgc agcggcgccg cctggatcac gggggcattg gcgtggacgt aggcgtgcgc
     3301 ggagtcgggc ggttccgccc ccgcggccgg agccagcaga agcagggcgc aaagaatgag
     3361 cgcgatcatg aacagccctc ggggtgtcga gacttcctgc gtccggcgtc gacgcgacgc
     3421 actcgtgaga cagcccgccc ggcgccaggg ttggaatccg ccgagccggc atggccgcgt
     3481 ccacacccag ccttacgatc ccgccgcgcg tccgggtttc ggtccgtgta tcctcgacgc
     3541 tccgaccggc acgcccacgg aggcatccga ctatggcgcg gcaacccgcc cgacgctacc
     3601 gcttcatcgt gtccctgacc ttcctgatcc cgatgctcgt ctgcctcgcc gccccgccag
     3661 tcctggccgc ccccgaacag ggcgagcggg gacgctcgcc ggcctcggtc gtcacccgcg
     3721 agcgttccgt caccgaggtg ggcgcaggga tctacgtgat ccgtcatccg gacggagccg
     3781 atacgaatcc ccaaggcaac acgacggtgg tcatcggatc ccgggacgtg ctggtggtgg
     3841 attcgggcta cctgccctcg agcgcccgcg acgatatcgc acagatccgc cgctggacga
     3901 agaaaccggt gcgctacctg gtcaacaccc attggcaccc ggaccacgtg cgcgggaacg
     3961 ccgcttacgc cgcggccttc ccggggctcg cgatcatcgc gcatgaaacc acgcccgagc
     4021 tggagcgggg attcgacgag ccgaaccttg cgcgctaccg ggcgcgcgtg gagacgctcc
     4081 gtcacgagct gagcaccggc cgcgcgccgg acggcaagcc gctgagcgac acggagcgta
     4141 cggaggcggg cgcggacctg gcgcgccgcc tgccggtcct gcgcgagttc gaggggtacg
     4201 agccggtcta tccgtccgtc accttcgccg acgccatgac cctggatctc ggcggccgga
     4261 cggtccagct ccggcacccg ggccggggac atacggtggg cgacgtgatc gcgtggctgc
     4321 cccgcgagcg ggtcctgatc gcgggagatc tcgtgaccca tcccgtcccc tacttcttcg
     4381 cgggatggcc gtacgacgag atcgacacgc tcgagcgcct ggccgccctg gacgcccgcg
     4441 tcatcgtccc cggccatggc caggtcctgc atgaccggcg ctacctcgac cagacgctgg
     4501 agctgttgcg gagcgtccgg gcccaggtcg tcgccgaggt tcgcaagcgg ggatcccttt
     4561 cggcgaagct ggaagacgtg cgccgggcga tcgacttcgg agcggcggag cggcggtttg
     4621 cgggaagcga ttccgagagc gtggagttct tccgggagtc gatggacggg ctggtgaaga
     4681 ccttcttcta ccagatcgcg gagtagacct gtgatgcggt acaatagagg ctcccgcgac
     4741 ctccggcacc atccccgcta tccggctccc gaggccgggg cgtcgtgcgg cgttctggag
     4801 gtccgcatga gacgcgcccc gctaccccac ggtcctcgaa ccccgatcct ggtcttccgc
     4861 gcggccctgg ccctggctgc agccctcgtg ctcgccacgg ccgcggcagg cagggccacg
     4921 gcggcgcccc cctgcgcgcg cacggccggg cagagcgcgt gggcgttcct ttggaccccc
     4981 acctggcttc ccggtgtccc ccaagccgca ggaaccgccc ttcagggcga cgccgcgacc
     5041 gccgggttgt ggatcgcgct cgatcccgta acccgccggc ccgtccgccc caccccggag
     5101 cagcgccgcg acgcggggct cgccgcgccg gaggtggacg aggtcctgcc ggtggagcgg
     5161 attcccggcg ggggcgagct gctccacctg aacgggcggc accaggtgtt cgaggtcgcc
     5221 cggcgcgacg cgacgggccg gttccggacc agctgcgcgt ccgattccgc cacggcaacg
     5281 cgggtgctcg cgacgcccgt atcggcgcgg gaggcgaaat gatccgcgcc ctgcacgccg
     5341 tgaccctcgg cgtggccgcc gcgatcctcc ttctggccgc gccgttcgcc gaagcgggat
     5401 ccttcgtcct catcaacctc gacggcgcgg gcgaggggtt caacgatccg acgcccgtct
     5461 ccccgatcgg tggaaacccg gggacgaccc tggggcagca gcggctcaac gtattccaga
     5521 ccgccgggaa gatctggggg gcgtacatcc ccgacaacgt gacgatccgc gtggaggcgc
     5581 gcttcgaccc gctcacgccg tgcgattcgg tgagcggtgt cctgggcagc gcgggggcga
     5641 tcacgctggc gagcgacttc gccggggcgc ccctggcaag cacctggtat ccgatcgccc
     5701 tcgcgaacaa gctggccgga accgacctgg ccaccaccgt caacgacatc accgctcact
     5761 tcaattccag cgtggacaac tcgacctgcc tgggcacctc cggctggtac tacggctacg
     5821 accacaacga ggggaccaac atcgacctcc tcgctgtcgt gctccacgag ctgagccacg
     5881 ggctggggtt ccagaccttc acgaatctct cgacgggggg attccagaac ggcaagccgg
     5941 acgtctacgc acgccacctc ttcgatcgga cgctgaacga gcactgggac cagatgacca
     6001 gcaaccagcg ctcgaactcg gcggtcaaca ccgggaacct ggtctgggac ggctccttcg
     6061 tcaccaacgc ggcccacctc ttcctggggc ccgcggccat cgtgcggatc gattcgccgg
     6121 ccgtgatcgc cggagagaag gactacggga cggccgattt cggcgccccg cctccgaacc
     6181 cgccgcttca gggccaggtc gtgctggtca acgacggggt gggcacgacc agcgacgcct
     6241 gcgagccgat cgtgaacggg gcgcagatcg ccgggaagat cgcgttgatc gaccggggaa
     6301 cgtgcacctt cgtggccaag gcggcggcgg cgcaggcggc cggcgcgatc gcggtcatca
     6361 tcggcaacaa cgtggccgga gcgcctcccg ggatgacggg ggtggatccg agcgtcacga
     6421 tccccgtcgt gagcatcacg caggccgatg cgaccgcgat caagggacag ctgggggtcg
     6481 gcgtcttcgc gaccatcggc atcgacgccg cgcatctcgc gggcaccgac acgcagggga
     6541 gggtgaagct ctacgcgccc aatccgctcg agtcaggatc gaacgtgtcg cacttcgacg
     6601 tctcggcgac gccgaatctg ctggaggagc cgtttatcaa ctccgacctg accgacagcc
     6661 ccgacctgaa ccagtacctg ttcgccgaca tcggatggtg gaccggcgcc gcggcggtcg
     6721 cgacggccca ggccgccggc gccccgcgcg cattcagcgc tcccaatccc ttctcggcgg
     6781 ggacggcgat ccgcttctcg ctcgcgcgct ccggcaagac cgacgtcgcg gtcttcgacg
     6841 ctcgtggggc attggtcaag cgccttgcct cctcatggcg tccggccggc gcgcagcggg
     6901 tggagtggga cggcaccgac gcgagcggcg ggcgggcccc cgcgggcgtc tattactggc
     6961 gcgtcacgtc ggaggaagcc ccgcagacgg gaagagtcgt tcggattccg tgagtccgca
     7021 agcgatacgc aagtagcgcg tgtcgcactc cggtgacatg cgttaagcgt ttcacacgcg
     7081 ccagcgcggg taaccccatt gcggcgggct acgagactac gaacccgact tctccggttc
     7141 gcacaccgga cagcccttgt cgaacacgac gcgccacttg ccgtcggatt cgagccgcca
     7201 gatcgtgctg aaggtgccga tcagggcgcc cttgggatct ttcaccggtc cactcgagag
     7261 cgcgagcttt cccgacttca agacctcgat cgtctccggc tcccaggaaa agggcgcgtc
     7321 cggaccgtcg aagaacttcg tccaggcttc gaccaccgcc ttgcgtcccc gggtcgcgcc
     7381 gtttcgcccg aagaacacgc cctccacggc gatgtagctg ccgaaggcct tgagatcccg
     7441 gtccgccatc gtcttcgcga acgcgcgctc ggacgcgcgc acctcttccg cgcggtcgcg
     7501 cggcgcggcg gacgcgagcg atgggagcat cgtgagcgcg gcgccgagga ccaggcaggc
     7561 cgcggcccag ccgaaaagag atcgcttcat cgttacctcc gggtgatccg gcgcgttttt
     7621 cggtgggggg atcctagcgt gaggccgcgc gtgccgccgc gagagaaaaa atagcacacg
     7681 gccggccgcc gcgtcgtcgt cgagaccagg ctcagctcga cagagattcc gtacctccct
     7741 acgacgggct cgatccgatc ctgcgcggcc acctcgagga ggactgggag agaatgacga
     7801 caggtgtcgc gggattcact ctcgccagcg gcggtgaaac catggacatc tgtcgcctga
     7861 ttcacacgcg ccagcgcggg tgaacgtgat gaccgatgtc gccgcgttca cactcgctca
     7921 gcgcgggtga tacggacgcc aggtgtcgca gagctctcgc gcaatcgccg cgccgtagcc
     7981 tcgttgttca accacgcgcc gcatcccacg gcgaccatcg atcgggccaa ggtcccatag
     8041 cggtcgcggg ccgcatggac gaactgcgcc agcggtgcat ccgttcggct cgggaggaac
     8101 gaagtttcag acgctcagtc ctcgaggccc agcttcttca agaaagcacc ccaccgcggg
     8161 tccgatcgga ccgaccggaa gtgcgggctc ggcttgatgt gcatgaggcc gctgtcccgc
     8221 cgctcgaagg cccgctcgag ccacgcgaac gcctcgtcgg catggccaca cgctccgtgg
     8281 gcctcggcca cctgcagccc gaactcctcg ccatgcctgg cggcaagctc acggatcgcc
     8341 tccagcgatt ccgcctcgcg atcctgccga tggtggatga tcgcgagcgt gaggaggcgg
     8401 tagacgtcgt cgggctcgcg gaccgcctcc tccaacgcct cctcgccccg cccctcttcc
     8461 atcaggacct gagccaggaa cgagcgcgtg ccatagcggt tcggcgaaag ctccagggcc
     8521 atccggagtg cggcctccgc ctcggccagg cggcccgact cgtagtagac gcaccccagg
     8581 ttgttctgcg acgccgcgct caagggatcc agcgcgatcg cgcgcaggtt catccgcacc
     8641 gcctcgtccg accgccccag cgcttgaagc aggatcgccg cgccatggag cgcggtcgcg
     8701 ttgttggggt cgcgatcgag cgcccgcagg aaggacgcct cggcgcgcgc ccagtcccgg
     8761 tcgtgcacga actggatccg ggccatccgc gcgtgcgccg aggcgagatc gggctcgagc
     8821 gtcagggcgc gcccgaccgc ctcgcgcgcc cgcgcgtagc cgtcctcgtt ggagcgcatg
     8881 ccgaagtagg cctcgtcggc atgggcgttt cccagctcgg cccacgcgag cgcgtcggat
     8941 ggatcgcgcg ccacggcttc gcgcaggagc ccgatcgcgc gcgtgagatc ctcgggcgtc
     9001 ttacggttgg caaagaagcg tccctggaga tagaggcggt gcgcttccgg gtcgcgcccg
     9061 cggccgcgca ccgcgtcggc gacttcggcc cggatcgcct cgagcgccgc gccgggcgca
     9121 ccgcccggga gcagtgcggc gcggagctcc tggaccacgc actgggcgat gtcgtcctgc
     9181 accgcgaaga tgtcgtccaa gcggcggtcg aaggtctcgg cccagagctg gtccccatcc
     9241 gaggcgcgca cgagacggac ggagatccgc acgcggtcac cggccttgcg cacgcttccc
     9301 tcgaggaccg tctcgacgcc cagcttccgg gcgatgaccg cgaggtcctc gctctttccc
     9361 ttgaactgga aggccgaggt gcgcgcggcc acgtggaggc cccggatcgt ggagagcacg
     9421 ctgatcatct cgtcggccaa tccgtccgaa aaatactcgt ccgcctcgtc gctgcggttc
     9481 aggaacggca gcaccgcgac cgacgcgcgg cgcgagcgcg agcgggctcc gcggcgttgg
     9541 ccatccggtt cctcgtccgc gatcggcgcg agcagcgccc gcatcgccga cgcggtgaag
     9601 ggaccggggc gctccggccc cgagctcatc gcgcgctcga tcgcggcgat cagcccggcg
     9661 ggcgcgtcgg gccgctcgct cgagagcggg cgcgcggggc cgcgcagggc ctcctgccgc
     9721 agctcctcca gcgtgcgcgc gtggaatggg ggatgtccgg tcagggccca gcgaagcgtc
     9781 acccccagcg cgtagaggtc ggtccgcggc gtcggcggcc cgccgtcgag gaggtcgggc
     9841 gacatgaaga gcggcgtccc ggagcccttc cgcggcgcgt cggcgggaat cagccgccgc
     9901 ctccacccca ggccgaaatc ggtgagcacg acacgcccgt ccgattcgag cacgacgttg
     9961 gccggcttga tgtcgcgatg gaccagcccg gcctgatcga tcgcctcgag cgcggcgcag
    10021 agctgggccc cgatgcgcgc ggtctccgcc ggcgagagcg cccccctggt ttcgatctcg
    10081 ctcgcgagcg cgcggccgcg gagaagctcc atccacatgc cgggacgtcc gtcgagctcg
    10141 gcgatgccgt agacggcgac gacgctgggg tggcgcacgc gggcgagcgc gcgcgcttcg
    10201 ccgaggagag cggccgaggg tccggcgccc ggagccgtgg acgcgccgtc ggcccctggc
    10261 gcggctgcgc gggcctgcag gaacttgacc gcgacttccc gatggaggga agcgtcccac
    10321 gcgcgccaca cctcgccggc cgcgcccgcg ccgatcggct cgagcaaggt gaagtggccc
    10381 caccgccccg gctcgtcggc gccgccggtc tgttccacgc ccgttcccgc ctcggcttcg
    10441 gcgtccgcac cgtcgccctc ggggcctcgc tggagcgcgc ggttgaacgc ggcgatcctc
    10501 gcgaggtcct ggagcgcgcg gaaggaggca tcggtctcgg gcgagagcgc gtcggtgcgc
    10561 gcggcgtccc aatcgatcgg cgcgccgtcg gagaccgagc tggccagctc gtccaggctg
    10621 tcgtgcagcg gatcgtcagc gttcatggcc catgatgtcg gcgagcttgc gcagggcgcg
    10681 ctggaccgcc atgcgcgcgg cgtcgcgggt cgcccgttcc gtcacggcgg cgatttcctc
    10741 gtagtcgaaa tcgagctcga tgcggaggtg aaggagctgc cgttcgtcgt cggtcagacg
    10801 cgcgagcgcg ccctcgaagc gccggaggac gtccgagccg atggcgctct ccagcggcga
    10861 ggggctcacg tcgatcaggt cctcggggac cccgtccgga cccggccgcc gtcgagccca
    10921 gcgcacctga tccttgatac ggttgagcac cgcctggcga acgtaggcct gaaagactcc
    10981 gggcccgcgc acctcgatca ggtccagacc ttcgatcgcc ttgaggagcg tctcctggac
    11041 caggtccccc gtgtcgaaga gcgatcgcgc gtagcgcgga agccgcccgg tggcccagcg
    11101 ctggaggcgc ggacggtagc gggccatgag cgcgttcaga gcggcctcat ccccatgctt
    11161 ggcgcgatgg aggagctcgc ccgtgatcac gagctcgggc gcgggcgagt cgg
//
LOCUS       DASSLZ010000283         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1496800, whole genome shotgun sequence.
ACCESSION   DASSLZ010000283 DASSLZ010000000
VERSION     DASSLZ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496800"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="VFM00_10775"
     CDS             complement(<1..1470)
                     /locus_tag="VFM00_10775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7498773.1"
                     /translation="MIAIICFAALLRLWGIQDRLPDPTIGINVLEDSAIEETDRTTMG
                     RAWAMWRGGVDRLDLNPHTGGWPALSFYTTLATQELYRGYYGVTHPGSSAAQFSDYVV
                     GGYGARDLFILARLLGALVGIVTVALTYRLGMLVGGRFLAIGAGLLLAVNLPHILVSQ
                     HVSDPNLLGLLFVLIACFPMVRIARGRGRTRDSVLAGAMIGLAGASKYVPLVVGLPYL
                     FAHPRGLKNRALWLGIAAAAVALFIATPYTFLDWRTTLRDLDRQKASLFSSWVGQSTF
                     PISLPTYLIASLPHALGWPAYLLAIAGSAVLWRRGRAERILVGTAGVLIVVNGMLKAA
                     QERYVLVAMPLLVLAAAAGFEWALGLWRARAGARFTGRSALAVPALVATFAVAWPLPE
                     LAQTRATLRLPDTRHIARKWIQANLPTDAPAVVELYGPVFGEPERVFLIWPFFATQAP
                     LVRPAYHPEFLDGIRYYVQSSGISSRFEGDSANYPVEVAF"
     gene            complement(1571..1720)
                     /locus_tag="VFM00_10780"
     CDS             complement(1571..1720)
                     /locus_tag="VFM00_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498774.1"
                     /translation="MIRKLKTGEYRLYSRKADPKTKKRRNLGTFKSRAAAEKHEREVQ
                     YFKKH"
     gene            2045..2341
                     /gene="groES"
                     /locus_tag="VFM00_10785"
     CDS             2045..2341
                     /gene="groES"
                     /locus_tag="VFM00_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET7498775.1"
                     /translation="MNVKPLADRILVRRLEEPEMKRGGIIIPDTAKEKPQQGEVVAVG
                     PGRMTEEGKRVALDVKKGDKILMGKYSGTEVKIDGTDYLIMREEEVLAIVAESK"
     gene            2375..>3206
                     /gene="groEL"
                     /locus_tag="VFM00_10790"
     CDS             2375..>3206
                     /gene="groEL"
                     /locus_tag="VFM00_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET7498776.1"
                     /translation="MAAKEILFDEKARQAILSGVQTLAKAVKVTLGPRGRNVVLDKKW
                     GSPTITKDGVTVAKEIELEDRYENMGAQMVREVATKTSEVAGDGTTTATVLAESIFRE
                     GLRNVTAGSNPMGIKRGIDRAVKIVVEELKKISKPVKDPKEIMQVATISANGDEEIGK
                     IIAEAMEKVGKDGTITVEEAKSMDTTLEVVEGMQFDKGYISPYFVTDKEGMESVLEDA
                     YVLIYEKKLSNMKDLLPVLEKVAQKGKPFLVIAEDVEGEALATLVVNKLKGTIQVLAV
                     K"
BASE COUNT          621 a         1009 c         1130 g          446 t
ORIGIN      
        1 gaaggcgacc tcgacggggt agttcgcgga atcgccctcg aagcggctgg agatgccgct
       61 cgactgcacg tagtagcgga tgccgtccag gaactccggg tggtacgccg gtcgcacgag
      121 gggcgcctgc gtcgcgaaga agggccagat caggaacacc cgctcgggct ccccgaacac
      181 ggggccgtac agctcgacca ccgcgggcgc gtcggtggga aggttcgcct ggatccactt
      241 ccgcgcgatg tggcgcgtgt cgggaagccg gagcgtggcg cgggtctggg ccaactcggg
      301 gagcggccat gcgaccgcga aggtcgcgac cagcgcgggg acggccagcg cggagcgccc
      361 cgtgaagcgc gcgcccgcgc gggcgcgcca gaggcccagc gcccattcga atccggccgc
      421 cgccgccagc accaacaacg gcatcgccac caggacgtag cgctcctgcg ccgccttcag
      481 cataccattc acgacgatca gcacgcccgc ggtgccgacc aggatccgct cggcgcggcc
      541 gcgccgccag aggacggcgg agcccgcgat cgcgagaagg taggccggcc atcccagcgc
      601 gtgcggcagc gaggcgatca ggtaggtcgg aagcgagatc gggaacgtgg actgcccgac
      661 ccagctggag aagaggctcg ctttctggcg gtcgagatcc cggagcgtgg tcctccaatc
      721 gaggaacgtg tacggagtcg cgatgaagag agcgaccgcg gcggccgcga tcccgagcca
      781 cagcgcccgg ttcttcagcc cgcgcggatg ggcgaagaga tagggaagcc ccacgacgag
      841 cgggacgtac ttgctcgccc ccgcgaggcc gatcatcgct cccgccagga ccgagtcgcg
      901 ggtccgtccc ctcccgcggg cgatccgcac catcgggaag caggcgatca gcacgaagag
      961 gagcccgagc aggttgggat cgctcacgtg ctgcgagacc aggatgtgcg gcaggttcac
     1021 ggcgaggagg agccccgccc cgatggccag aaagcggccg ccgacgagca tccccaggcg
     1081 ataggtcagg gccaccgtga cgatgccgac cagggcgccg aggaggcgcg ccaggatgaa
     1141 caggtcgcgc gcgccgtagc cgcccaccac gtagtccgag aactgggcgg cgctcgagcc
     1201 ggggtgggtc accccgtagt agcccctgta gagctcctgg gtcgcgagcg tggtgtagaa
     1261 ggagagcgcg ggccatccgc cggtgtgggg gttcaggtcg agccggtcca ccccgccgcg
     1321 ccacatggcc cacgcccggc ccatcgtcgt gcggtcggtc tcctcgatcg cgctgtcctc
     1381 gaggacgttg atcccgatgg tggggtcggg cagccggtcc tggatgcccc acaggcggag
     1441 cagggccgcg aagcagatga ttgcgatcaa ccagcggtcg agaggagggc gcggcttctt
     1501 ggtcttgggc gcgggtcccc gctggcgggt ggcggcggcc ccggccgcgc gagaccggcg
     1561 ccgcggcccg tcagtgcttc ttgaagtact ggacctcgcg ctcatgcttt tccgccgcgg
     1621 ccctcgattt gaaggtgccc aggttcctgc gcttcttggt cttgggatcg gccttccgtg
     1681 agtagagccg gtactcgccg gtcttcagct ttcggatcat gacacgctcc ttccgcgcct
     1741 gcccgcggtc cacccccagg gtcccgagtg cggaacccct ttcagcatcg tagcttctcc
     1801 gggccgaagg ccgaagcgtc ccgttggcga gcgctgccaa ttggacatat gtagctcgcc
     1861 gaaggcgagc cagtggagga gcgcgatctc atgtaacgtt cataatgaaa agatgttgcg
     1921 gcttcgccgc aagacatcgg cacgccgatt gcgctacatg agaccgtcgt cggggcgcag
     1981 cctcccaggc tgcccatttg tcacaatcag aacatcccaa acgggacaag aaggagtcac
     2041 actcatgaac gtgaagccgc ttgcggatcg gatcctcgtc cgccgtctgg aggagcccga
     2101 aatgaagcgc gggggcatca tcatcccgga caccgccaag gaaaagccgc agcagggcga
     2161 ggtcgtcgcg gtgggaccgg gccgcatgac cgaagagggg aagcgcgtgg ccctggacgt
     2221 caagaagggc gacaagatcc tcatgggcaa gtactcgggc accgaagtca agatcgacgg
     2281 gaccgattac ctcatcatgc gcgaggagga agttctcgcg atcgtcgccg aatcgaagta
     2341 agggcgaggg cctcaaataa aggagtgatc cagaatggct gctaaggaaa ttctcttcga
     2401 cgagaaggcc cgccaggcca tcctctccgg cgttcagacg ctggcgaagg cggtcaaggt
     2461 gacgctcggt ccgcgcggcc ggaacgtcgt gctcgacaag aagtggggat cgccgacgat
     2521 caccaaggac ggcgtgacgg tggcgaagga gatcgagctc gaggaccgct acgagaacat
     2581 gggcgcgcag atggtgcgcg aggtcgccac gaagacctcc gaggtcgcgg gcgacggcac
     2641 gacgacggcg acggtgctcg ccgagtcgat cttccgggaa ggcctccgca acgtgacggc
     2701 cggctcgaac ccgatgggca tcaagcgcgg catcgatcgc gcggtcaaga tcgtcgtgga
     2761 agagctgaag aagatctcca agccggtcaa ggaccccaag gagatcatgc aggtcgcgac
     2821 catctccgcc aacggcgacg aagagatcgg caagatcatc gccgaggcca tggagaaggt
     2881 cggcaaggac ggcacgatca cggtcgagga agccaagtcg atggacacga ccctcgaagt
     2941 cgtcgagggg atgcagttcg acaagggcta catctcgccg tacttcgtga cggacaagga
     3001 agggatggag tcggtcctcg aggacgccta cgtcctgatc tacgagaaga agctctcgaa
     3061 catgaaggac ctgctcccgg tgctcgagaa ggtcgcccag aaggggaagc ccttcctggt
     3121 gatcgccgag gacgtcgagg gtgaggcgct cgcgaccctg gtcgtgaaca agctcaaggg
     3181 caccatccag gtgctggccg tcaagg
//
LOCUS       DASSLZ010000284         8321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_485660, whole genome shotgun sequence.
ACCESSION   DASSLZ010000284 DASSLZ010000000
VERSION     DASSLZ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485660"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1284
                     /locus_tag="VFM00_10795"
     CDS             103..1284
                     /locus_tag="VFM00_10795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7498777.1"
                     /translation="METAATLNPPQPVQTDSTTRRVLILYVRAGVGHERAARAVAQAM
                     RETDPHSEPILHDALDFSTWPLRWLYASSYNRMLSRTPRVWGALYRRAGTRPHGFRAR
                     SRTRVTMLFCKDFLGAVERYKPDSVLCTQFLPSEVYATLRDEGELKVPVYTVVTDFMI
                     HPIWVYRGMDRYFVATQTTKDQLVDTGEVPPERVQVTGIPIDPKFAVPIAKEDARRQL
                     GLDTDPSRPVVLVMSGGFGWGPVEQVLEVVLSLPDRVQAAVVCGRNEGLCRKLSHRVR
                     GLEDRIKISGFTDRVDLYMAAADVMVGKSGGLTMSEAMARGLPMIVFRPIPGQEERNC
                     DFLQETGAGVRVHDYEELHYRLMSYVLHPEHLEVMRGAAARIGKPRSSHDVAQTVLRG
                     P"
     gene            complement(1304..2530)
                     /locus_tag="VFM00_10800"
     CDS             complement(1304..2530)
                     /locus_tag="VFM00_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498778.1"
                     /translation="MLRAPNRCSTAPVLAILFCSIVSTPALAQPLAAISTFFDPSIES
                     SGMGYASVAVPWQDDLNDWANPALLGYQRGIRYSRGKTQLVPDLADDVFFTSDRFAVG
                     ACGLGVSLVGKPIDGLGKLRLDYGLSEATDVNGNVIGTFDSFEEVHQIGVGMNVLQFL
                     ENVTQARGGRHQHLSRYADLSLGHTWKSIVIDLAPAGVTLDGLGGRGEAHEKDRGALL
                     RVTPVGAAGTLADGSGRIKLDVSGAFSQRNYGDATISFIDVDNVDPISEERLVGAATR
                     LSFRVDAAPGGIWDLLSPLVSVGGAWEEARYYSGEIKVGGTIHRTGQVIDILGLVSLR
                     HGFVDDPRGEIQDDSWGYSVGLQYRGIVAARYDWAEIPQSSFLTRIHREGFTVQFDPY
                     RLYRATRASGESVAAN"
     gene            complement(2675..3091)
                     /locus_tag="VFM00_10805"
     CDS             complement(2675..3091)
                     /locus_tag="VFM00_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498779.1"
                     /translation="MDSFESFEQAACKAFDFLCDRFGFQSPEIERLGRESYVHFQKGP
                     RIVSIAWEPGTPPIVELFLPTEGSGLPATPWAERDGVPYSRRFPGRIGSGPGSSNREG
                     PRRGPHAWKQPTAVMFEEYLAEEARNLEDAELIFIS"
     gene            3209..3793
                     /locus_tag="VFM00_10810"
     CDS             3209..3793
                     /locus_tag="VFM00_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET7498780.1"
                     /translation="MPETIATDVITGESGLSSEPRETRKGVLLVVTGTGKGKTTSAFG
                     TALRGLGHGFKVAVVQFMKGRIYGELLVLRDRLGVDVWQFGRNAFVDPKNPDPRDLQL
                     AREGLDKAWEIVRGGQHDVLILDEIIYVASYGLVPEHEVLALARARPRWMDLILTGRG
                     ASAELIELADTVSEVKEIKHHYKKGIESRAGMEY"
     gene            3868..4827
                     /gene="meaB"
                     /locus_tag="VFM00_10815"
     CDS             3868..4827
                     /gene="meaB"
                     /locus_tag="VFM00_10815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET7498781.1"
                     /translation="MPTALGKEILSGDSRALARGMSLVENGAAEATVLLDALYGKKPA
                     APRIGFTGPPGAGKSTLVAEYARLLRAQGRQVGVIAVDPTSPFTGGAILGDRVRMSDH
                     ATDGKVFIRSMATRGSLGGLAARTGELAELLDAWGSETTLIETVGVGQSELDVAETAD
                     TTVVVLTPESGDAIQTLKAGLMEIADVLVLNKADHPGADPMEAALRSSLAFRPKGGFS
                     PPIVRTIATERNGIEAMHAAIQEHRAWAERPEQAEIARRERARARIRLAVREGLLDAW
                     AARQGLESWIEAAADQVLAGGLSPYRAARDVIERVTRHGGNAS"
     gene            4835..6487
                     /locus_tag="VFM00_10820"
     CDS             4835..6487
                     /locus_tag="VFM00_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET7498782.1"
                     /translation="MKSATAPAAGTKPAHGAADGAIPGIPPETLSGIPVKPLYTETDL
                     PGDVEKRLTPPGEFPYLRGLHPGMYRTRLWTMRQFAGYGSAAHTNERFHYLLKHGGTG
                     LSTAFDMPTLMGYDADHPMSLGEVGREGVAVSTLEDMEILFKGIPLDEVTTSMTINAP
                     AIVLLAMYVAVGERHGIPAAKLGGTVQADMLKEYIAQKEWIIPPRPAVKLCVDMIEWC
                     AANMPKWNSISISGYHIREAGATAAQELAFTLADGIGYVEECVKRGMDVDSFAPRLSF
                     FWDVHNDFFEEIAKFRAGRKIWATVMRDRFHAKNARSLMLRTHAQTAGVSLTAQQPLN
                     NVARVAIQALAAVLGGTQSLHTNSMDETLALPTEEAATVALRTQQIIAYETGVTNTPD
                     PLAGSYFVEALTARIEEEALAYIRRIDEMGGIIVAVEKGYPQKEIADSAYQFQQQVER
                     GERVIVGVNKFQAENEEITIPVLKIDPAIERGQIERVRAVRARRAKAPYEQSMAALRD
                     ACMSTKNLMPYVLDAVRAEATLGEICDVFREAYGVYREPAVF"
     gene            6513..6935
                     /locus_tag="VFM00_10825"
     CDS             6513..6935
                     /locus_tag="VFM00_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET7498783.1"
                     /translation="MAKKIRVLVAKAGLDGHDRGAKVVASAMRDAGFEVIYTGLHQTP
                     EMIVQAAVQEDVDAVALSILSGAHMTLFPRVLELMREKGLNDVLLTGGGIIPKEDMET
                     LAKQGVGELFGPGTPTGAIIEYVRGWVAKHRSPAGSAT"
     gene            6893..7648
                     /locus_tag="VFM00_10830"
     CDS             6893..7648
                     /locus_tag="VFM00_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019259884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HET7498784.1"
                     /translation="MGREAPLSRGQRHVNKVVQDAREAIERAGIKDGATIVFGGFGLC
                     GIPENLIVALKDSGVKGLTCVSNNAGVDDWGLGLLLQTRQIKKMISSYVGENATFERQ
                     FLSGELEVELCPQGTLAERIRAGGAGIGGFFTPTGVGTVVAEGKETRTIQGREYVLEM
                     PIRADFAFVRAHIGDRMGNLVYRMSARNFNPMAATSGRVTLAEVENLVEPGAIDPDHV
                     VTPGIFINHIFQGTYGPKRIEKRTNRPASGARA"
     gene            7590..8252
                     /locus_tag="VFM00_10835"
     CDS             7590..8252
                     /locus_tag="VFM00_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HET7498785.1"
                     /translation="MDPSESKRERIVRRAALELKDGFYVNLGIGMPTLVSNYIPQGMN
                     VILQSENGVLGVGPYPEAGQEDADLINAGKETITVLPGAAFFSSADSFAMVRGGHVDL
                     TILGAMEVDQEGNLANWMIPGKMVKGMGGAMDLVAGAKRVVVTMFHTAKGDVSKVLKK
                     CALPLTGRKVVNRIITDLAVLDVTPRGLRLVERAPGVTVDEILKSTEADLVVEEVAEM
                     PV"
BASE COUNT         1440 a         2753 c         2855 g         1273 t
ORIGIN      
        1 agggggcctc cgggccagtc agaagggtcg aaaaatcccc cggcacgcgg tatcgtcgcc
       61 ccgtcgctcg gacctcgctc gtcccggttc gttcggaggc gcatggagac cgcagccacg
      121 ttgaatccgc cccagcccgt gcagaccgac agcaccacac gccgcgttct catcctctac
      181 gtccgcgccg gcgtgggcca cgagcgcgcc gcgcgcgcgg tggcgcaggc catgcgcgag
      241 accgatcccc attccgaacc gatcctccat gacgcgctcg atttctccac gtggccgctg
      301 cgatggctgt acgcctcctc gtacaaccgc atgctctccc ggaccccgcg cgtgtggggc
      361 gcgctgtatc gccgggcggg cacccgcccc cacggattcc gcgcccgctc ccgcacgcgg
      421 gtcacgatgc tcttctgcaa ggacttcctg ggcgcggtgg agcggtacaa gcccgactcc
      481 gttctctgca cgcagttcct gccctccgag gtctacgcca cgctgcgcga cgagggagag
      541 ctgaaggtcc cggtgtacac ggtggtcacc gatttcatga tccatcccat ctgggtctat
      601 cgcgggatgg accgctactt cgtcgcgacg cagacgacca aggatcagct cgtggatacg
      661 ggcgaggtcc cgcccgagcg cgtgcaggtg acgggcatcc ccatcgatcc caagttcgcg
      721 gtgccgatcg cgaaagagga cgcccgccgg cagctcggcc tcgataccga tccgtcacgc
      781 cccgtcgtgc tggtgatgag cggaggcttc ggctggggcc ccgtggagca ggtgctcgag
      841 gtcgtgctgt ccttgcccga ccgcgtgcag gcggccgtcg tgtgcgggcg aaacgagggc
      901 ctgtgccgga agctgtcgca tcgcgtgcgc gggctggagg accggatcaa gatctccggc
      961 ttcaccgatc gcgtggacct ctacatggcc gcggccgacg tgatggtggg gaagtccggc
     1021 ggattgacca tgagcgaggc gatggcccgc gggctaccga tgatcgtgtt ccgccccatt
     1081 cccggccagg aggagcgtaa ctgcgacttc ctgcaagaga ccggagcggg cgtgcgcgtg
     1141 cacgactacg aggagctgca ctaccggctg atgagctacg tattgcaccc ggagcatctg
     1201 gaggtcatgc gcggggcagc ggcgaggatt ggcaagccgc gatcgtcgca tgacgtcgct
     1261 caaaccgtac tgcgcggacc ctagccgatc gcgctctgcg cgatcagttc gcggcgaccg
     1321 attctccgct ggcccgagtc gcccggtaca gacggtaggg atcgaactgg acggtgaacc
     1381 cttcgcggtg aatacgagtc aggaaggacg actgcgggat ctccgcccag tcgtatcgtg
     1441 cggccacgat cccacggtac tgaagtccca cactgtagcc ccagctgtcg tcctggatct
     1501 ctccgcgcgg atcgtcgaca aatccgtgcc ggagcgagac caggcccaga atgtcgatca
     1561 cctggccggt cctgtggatc gttcctccca ccttgatctc tccactgtag tagcgcgctt
     1621 cctcccacgc gcctccgacg ctgacgaggg gggagagcag gtcccagatc cctcccggag
     1681 ccgcgtccac gcggaacgaa agccgtgtcg cggctcccac caggcgctct tcgctgatcg
     1741 gatccacgtt gtcgacgtcg atgaaactga tggtggcgtc gccgtagttc cgttgtgaga
     1801 acgccccgga aacatcgagc ttgatgcgtc ccgatccgtc cgcaagggtt cccgccgctc
     1861 cgacgggcgt gacccggagg agcgcgccac ggtccttctc gtgtgcttcg ccccgtccgc
     1921 ccaggccgtc gagcgtgacg cctgcaggcg ccaggtcgat gacgatcgat ttccaggtat
     1981 gtccgagcga caggtcggcg tatcggctca ggtgctggtg tcggccgccg cgcgcttgcg
     2041 tcacattctc caggaattgg aggacgttca ttccgacgcc gatctgatgg acctcctcga
     2101 aggagtcgaa ggtcccgatg acattcccgt tgacgtcggt cgcttcgctc agcccgtaat
     2161 cgaggcgcag cttcccgagg ccgtcgatcg gcttgcccac gagggaaacg ccaagcccgc
     2221 aggcgcccac ggcgaagcgg tcgctcgtga agaagacgtc atcggccaga tcgggaacga
     2281 gctgggtctt gccgcggctg tatcggatcc cccgctggta gccgaggagc gccgggttcg
     2341 cccaatcgtt caggtcgtcc tgccagggca cggcgacact cgcgtagccc atcccgctcg
     2401 actcgatcga ggggtcgaag aacgtggaga tcgcggctag cggctgcgcg agcgccgggg
     2461 tggagacgat cgagcagaag agaatcgcga gcaccggtgc ggtgctgcaa cggttcggag
     2521 cccggagcat gctggcctcc tcggagtagg cggtggcgcc gcgacgcttg ggggatagtg
     2581 aaccgtagca atgccgcgcg ccccggcata ggtctactga agtacccctc ccgcggggtg
     2641 gcccgcgccg attgcctttg cccggtgtcc gaaactacga aatgaaaatg agttcggcgt
     2701 cctcgaggtt ccgagcctct tctgctagat attcctcgaa catcactgcg gtcggctgct
     2761 tccaggcatg gggacctcgt cgggggcctt cccgattgct cgaccccggc cccgatccaa
     2821 tgcgaccagg aaaccggcgc gagtacggaa cgccatcccg ctccgcccac ggcgtcgccg
     2881 gcaggccgct tccctcggtc gggaggaaca gctccacgat gggcggtgtg cccggctccc
     2941 acgcgatcga cacgatgcga ggtcccttct gaaagtggac atacgattcg cggccgagcc
     3001 gctcgatctc cggcgattga aatccgaagc ggtcgcacag gaagtcgaac gctttgcagg
     3061 cggcttgttc gaacgattcg aaagagtcca tttccgtcca gggtagaggc ttgacgtcgt
     3121 gcgtgccatc ttggaggcgc cctggtcatc gaggcgccga cccggggctc aggttgacgc
     3181 ggcaccggta gcctgctacc atccttcgat gccggaaacc atcgcaactg acgtaatcac
     3241 aggggaatcg gggctctcta gcgaaccccg cgagacccgc aagggggtcc ttctcgtcgt
     3301 cacggggacc ggcaagggga aaaccacctc cgcctttggc acggcgctcc gcggattagg
     3361 ccatggcttc aaggtcgcgg tcgtccagtt catgaagggg cgcatctatg gcgagctgct
     3421 cgtgcttcgc gaccggctcg gcgtggatgt gtggcagttc gggcggaacg ccttcgtcga
     3481 tccgaagaat cccgacccgc gcgaccttca gctggcgcgc gagggcctcg ataaagcctg
     3541 ggaaatcgtc cgcggcggcc agcacgacgt cctcatcctc gatgaaatca tctatgtggc
     3601 gagctacggc ctcgtacccg agcacgaagt gctcgcgctg gcgcgtgcgc ggccacgatg
     3661 gatggatctc atcctgacgg gacgcggcgc gtcggccgag ctcatcgaac tggccgacac
     3721 ggtcagcgaa gtgaaagaga tcaagcacca ctacaagaag ggaatcgaat cgcgcgccgg
     3781 gatggagtat tgaatccagc cccgccgcgc gcctctgggg ccccggccgc ggcggcttcg
     3841 gccggcgggg ctgctacctc cgcagcattg ccaaccgccc tgggcaagga gatcctgagc
     3901 ggcgattccc gggcgctcgc ccgcggcatg tcgctggtcg aaaacggcgc ggcggaagcg
     3961 accgtgctgc tcgacgcgct ctacgggaag aagccggcgg cgccgcgcat cgggttcacg
     4021 ggccctccgg gcgcggggaa gagcacgctg gtcgcggagt acgcgcggct tctgcgcgcg
     4081 cagggacgcc aggtgggcgt gatcgcggtg gatcccacga gccccttcac gggtggcgcg
     4141 atcctcggcg accgggtccg catgagcgac cacgcgacgg acggcaaggt cttcatccgg
     4201 agcatggcga cgcggggcag cttgggcggc ctcgcggcgc gcacgggcga gctggccgag
     4261 ctgctcgacg cgtggggcag cgagaccacg ctgatcgaaa cggtcggcgt ggggcagtcg
     4321 gagctggacg tcgccgagac ggcggacacg acggtggtcg tgctgacgcc ggaatcgggc
     4381 gacgccatcc agacgctcaa ggccggcctc atggagatcg ccgacgtgct ggtcctgaac
     4441 aaagcggacc accccggcgc cgaccccatg gaggcggcgc tccgctcctc gctcgcgttc
     4501 cggccgaagg ggggcttcag cccgccgatc gtgcgtacga tcgcgaccga gcggaacggc
     4561 atcgaagcga tgcacgccgc gatccaggaa caccgggcct gggccgaacg gccggagcag
     4621 gccgaaatag cgcgccggga gcgcgcgagg gcccggatcc ggctggccgt gcgcgaggga
     4681 cttctcgacg cctgggcggc ccgccagggg ctagaatcgt ggatcgaagc cgccgcagac
     4741 caggtcctgg cggggggtct ttcgccctac cgcgcggccc gcgacgtgat cgaaagggtg
     4801 acgcgacatg gcggaaacgc gagctgaatc caaaatgaag agcgcgacgg ccccggccgc
     4861 gggaacgaag cccgcgcacg gcgcggccga cggcgccatc cccggcattc cgcccgagac
     4921 cctctccggc atcccggtga agccgctcta caccgagacc gacctcccgg gggacgtcga
     4981 gaagcggctg acgcctccgg gcgagttccc ctacctgcgc gggttgcatc ccggcatgta
     5041 ccgcacgcgc ctctggacga tgcgccagtt cgcgggctac ggctccgccg cgcacacgaa
     5101 cgagcgcttc cattacctgt tgaagcacgg cggcaccggc ctctccaccg ccttcgacat
     5161 gccgacgctg atgggctacg acgccgacca cccgatgtcg ctgggcgaag tcgggcggga
     5221 aggggtcgcg gtctcgacgc tcgaggacat ggagatcctc ttcaaaggga tcccgctcga
     5281 cgaggtcacg acctccatga cgatcaacgc cccggccatc gtgctgctcg cgatgtacgt
     5341 ggcggtcgga gagcgccatg ggatccccgc ggccaagctg ggcggcacgg tgcaggccga
     5401 catgctcaag gagtacatcg cgcagaagga gtggatcatc ccgccgcgtc cggcggtcaa
     5461 gctctgcgtg gacatgatcg agtggtgcgc agcgaacatg ccgaagtgga attccatctc
     5521 gatcagcggc tatcacatcc gcgaggcggg cgcgacggcg gcccaggagt tggccttcac
     5581 gctggcggat gggatcggct acgtcgagga gtgcgtgaag cgcggcatgg acgtggactc
     5641 gttcgccccg cgcctctcct tcttctggga cgtgcacaac gacttcttcg aagagatcgc
     5701 gaagttccgc gccggccgga agatctgggc cacggtgatg cgcgaccgtt tccacgcgaa
     5761 gaacgcccgc tccctcatgc tccgcacgca cgcccagacc gccggcgtgt cgctcacggc
     5821 gcagcagccg ctgaacaacg tggcgcgcgt cgcgatccag gcgctggccg cggtgctggg
     5881 cggcacgcag tcgctccaca cgaactcgat ggacgagacg ctcgcgcttc ccaccgagga
     5941 agccgcgacc gtcgccctcc gcacccagca gatcatcgcc tacgagaccg gggtcacgaa
     6001 cacgcccgat cccctggcgg gctcctactt cgtcgaggcg ctcacggcca ggatcgaaga
     6061 ggaggcgctg gcgtatatcc gccgcatcga cgagatgggc gggatcatcg tggcggtgga
     6121 gaagggatat ccgcagaagg agatcgccga ctcggcctac cagttccagc agcaggtgga
     6181 acgcggcgag cgggtgatcg tcggcgtgaa caagttccag gccgagaacg aggagatcac
     6241 gatcccggtc ttgaagatcg atcccgcgat cgagcgcggc cagatcgagc gggtgcgcgc
     6301 cgtgcgcgcg cgccgcgcca aggcgcccta cgagcagtcc atggccgcgc tgcgcgacgc
     6361 gtgcatgagc acgaagaacc tcatgcccta cgtgctcgac gccgtgcgcg ccgaggccac
     6421 gctgggcgag atctgcgacg tgttccgcga ggcctacggt gtctatcgcg agcccgccgt
     6481 tttctgaggc ggcccggacc cagaggaacg tcatggcgaa gaagatccga gtgctggtgg
     6541 cgaaagcggg gctggacggg cacgaccgcg gcgcgaaggt ggtcgcgagc gcgatgcgcg
     6601 acgccggctt cgaggtgatc tacacgggcc tccaccagac gcccgagatg atcgtccagg
     6661 ccgcggtcca ggaggacgtg gacgcggtgg cgctctccat cctgagcggc gcccacatga
     6721 ccctcttccc gcgcgtgctc gagctgatgc gcgagaaggg gctgaacgac gtgctcctga
     6781 cgggtggcgg gatcattccc aaggaagaca tggaaacgct cgccaaacag ggcgtgggcg
     6841 agctgttcgg ccccggcacg ccgacgggcg ccatcatcga gtacgtgcgg ggatgggtcg
     6901 cgaagcaccg ctctcccgcg ggcagcgcca cgtgaacaag gtcgtccagg acgcgcgcga
     6961 agccatcgag cgcgccggca tcaaggacgg cgccaccatc gtgttcgggg gcttcggcct
     7021 ctgcggcatc cccgagaacc tgatcgtggc gctcaaggac agcggcgtga aaggcctgac
     7081 ctgcgtctcg aacaacgcgg gcgtggacga ctggggcctg ggccttctcc tccagacgcg
     7141 gcagatcaag aagatgatct cctcttacgt cggggagaac gcgaccttcg agcggcagtt
     7201 cctctccggc gagctggaag tggagctctg cccccagggc acgctcgccg agcggatccg
     7261 cgcgggcggc gccggaatcg gcggcttctt cacgccgacc ggcgtgggca ccgtcgtggc
     7321 cgaggggaag gagacgcgca cgatccaggg gcgcgagtac gtgctcgaga tgccgatccg
     7381 cgccgacttc gcgttcgtgc gcgcccacat cggcgaccgg atggggaatc tcgtctaccg
     7441 gatgtcggcg cgcaacttca acccgatggc cgccacgtcg gggcgcgtga cgctggccga
     7501 ggtggaaaac ctcgtggagc cgggcgcgat cgatccggac cacgtggtga cgcccgggat
     7561 cttcatcaac cacatcttcc aggggacgta tggacccaag cgaatcgaaa agagaacgaa
     7621 tcgtccggcg agcggcgctc gagcttaagg atgggttcta cgtcaacctg ggcatcggca
     7681 tgcccacgct cgtctcgaac tacatcccgc agggcatgaa cgtcattctc cagtcggaga
     7741 acggcgtcct gggggtgggg ccctaccccg aggccggcca ggaagacgcc gacctgatca
     7801 acgccggcaa ggagacgatc accgtccttc ccggcgccgc gttcttctcg agcgcggact
     7861 cgttcgcgat ggtccgcggc ggtcacgtgg acctgaccat cctgggcgcc atggaagtgg
     7921 accaggaggg gaacctcgcg aactggatga tccctggaaa gatggtgaag gggatgggtg
     7981 gcgcgatgga cctggtcgcg ggcgccaagc gcgtcgtggt gaccatgttc cacaccgcca
     8041 agggggacgt gagcaaggtg ctgaagaagt gcgcgctccc gctcacgggg cgcaaggtcg
     8101 tgaatcgcat catcaccgat ctcgccgtac tcgacgtcac cccgcgcggg ctccggctgg
     8161 tggagcgcgc cccgggcgtg accgtggacg aaatcctgaa atcgaccgag gccgacctcg
     8221 tggttgagga agtggccgaa atgccggtat gaccggttcg gggggagcca tgccgacgac
     8281 gaccaccgcc gggacacggc gtgacgacat ctacgagctg a
//
LOCUS       DASSLZ010000285         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1731257, whole genome shotgun sequence.
ACCESSION   DASSLZ010000285 DASSLZ010000000
VERSION     DASSLZ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731257"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFM00_10840"
     CDS             complement(<1..693)
                     /locus_tag="VFM00_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498786.1"
                     /translation="MKRVVLPLFALAVAFAAGPAAAQHQHGASPSSPAAIPMFTGLGE
                     VHHPIGTRSAKAQKLFDQGLRLCYAFNHDEAIRSFREAARLDSACAMPWWGIALALGP
                     NINMPMSPDAEKQALEALARAKALAGAAPEADRAYIEALAARYAEPAGENRAPRDSAY
                     ANAMRSLKSRYPDDPDASVLCAEALMDLRPWDLYDVNGAPKPGAEEIVSILEGVLQKN
                     PRHTGALHFYLHA"
     gene            811..1731
                     /locus_tag="VFM00_10845"
     CDS             811..1731
                     /locus_tag="VFM00_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET7498787.1"
                     /translation="MSVTRSVISWLATQRAFTRTVGGTGMRLGFARRFIAGETLEDAL
                     QAAASLNRCGLQVILNHLGEHVRNQDEARDSYRSYYEILSELSRRKLDATITVKPTQI
                     ALELDYDLCRELTLRLVRDAAAAGNFVEIDMEHSGTTEATVSLFEYIRREHDNVGLAV
                     QSCLRRTRADLERLKPLRPKIRLVKGAYLEPHDVAFVEKREVDESYSQLMRVLFSDGF
                     FPAIGTHDEKLLAEAKVLAKEYGRRPEEWEVQMLLGVRSNLQDELVREGYRMRVYVTY
                     GTEWVPYFMRRLAERPANVGFVLRSLMKGR"
     gene            complement(1728..2369)
                     /locus_tag="VFM00_10850"
     CDS             complement(1728..2369)
                     /locus_tag="VFM00_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7498788.1"
                     /translation="MRPRLITFDIFGTVLDWRRGLADALERLGVALDGDAFDQLIDAQ
                     ADLESGPFRSYADITAESLVRVLGVDAETARAIGRDAGTWPLYPDSRDALRRLRAVAP
                     CAATTNSDREHGEQVQAALGFRLDAWICAEDVGVYKPDARMWHAASSRLGVAPGPWWW
                     HVSAYADYDLATARGLGLTSVYVARPHARPGSPDLAVANLAELAERAERNEAG"
     gene            2434..2889
                     /locus_tag="VFM00_10855"
     CDS             2434..2889
                     /locus_tag="VFM00_10855"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7498789.1"
                     /translation="MREAERIADLHRRAFDGDSWHGPHVFEILEGIDAARAARKPVAG
                     AHSVWEVVLHMRAWEDVVVRRLRGEIVELTDAQDWPRVADPSAAAWKTAIVGLRETHD
                     ELARTIAAIPDERLETTAPGGRTTLYRLAHGAVHHALYHAGQLAILKKG"
BASE COUNT          464 a         1024 c         1060 g          420 t
ORIGIN      
        1 ggcgtgcagg tagaagtgga gggcgccggt gtggcgcggg ttcttctgga ggacgccctc
       61 gaggatcgac acgatctctt cggcgcccgg cttgggcgcg ccgttcacgt cgtagagatc
      121 ccacggccgg agatccatga gcgcctcggc gcacaggacc gaggcgtcgg gatcgtcggg
      181 gtagcggctc ttcagcgagc gcatcgcatt ggcataggcg ctgtcgcgag gcgcccgatt
      241 ctcgccggcg ggctcggcgt agcgggcggc gagcgcctcg atgtaggcgc ggtcggcctc
      301 gggggccgcg ccggcgagcg ccttggcgcg ggccagggcc tccagcgcct gcttctcggc
      361 gtcggggctc atcggcatgt tgatgttcgg cccgagggcc agcgcgatcc cccaccacgg
      421 catcgcgcac gcgctgtcga gccgcgccgc ctcacggaac gagcggatcg cctcgtcgtg
      481 attgaacgcg tagcagagac gcaggccctg gtcgaacagc ttctgggcct tggccgaccg
      541 ggttccgatg gggtggtgga cctcgcccag cccggtgaac atcggaatcg cggcgggcga
      601 ggacggcgag gcgccgtgct ggtgctgggc cgcggccggg cccgcggcga acgcgacggc
      661 gagagcgaac agcggcagaa cgactcgttt catgggcacc tcccatgggc ctaaaggatc
      721 atccgcttcg gatcggagga gtctaccaac gggaaacggc cttcggtata gtcagcccgc
      781 cttgaacccg agccctcgaa cgcaacggcc atgtcggtaa cccggagcgt catatcctgg
      841 ctcgccaccc agcgcgcctt cacgcgcacg gtcggcggca ccgggatgcg cctggggttc
      901 gcccgccgct tcatcgcggg ggagaccctg gaggacgcgc ttcaggcggc ggcttccctg
      961 aaccgctgcg gcctccaggt gatcctgaac cacctgggcg agcacgtccg aaaccaggac
     1021 gaagcccgcg actcctaccg ctcctactac gaaatcctga gcgagttgtc ccggcgaaag
     1081 ctggacgcca cgatcaccgt caagccgacg cagatcgcgc tggagctgga ctacgacctc
     1141 tgccgcgagc tgacgctccg gctggtgcgg gacgccgcgg cggcggggaa cttcgtcgag
     1201 atcgacatgg agcactcggg cacgaccgag gccaccgtgt cgctcttcga gtacatccgg
     1261 cgcgagcacg acaacgtggg gctggcggtc cagtcctgcc tgcgccggac gcgcgccgac
     1321 ctggagcggc tgaagccgct ccgcccgaag atccgcctgg tgaaaggggc ctatctggag
     1381 ccgcacgacg tcgccttcgt cgagaagcgc gaggtcgacg agtcctacag ccagctcatg
     1441 cgcgtgctct tctcggatgg cttcttcccg gcgatcggca cgcacgacga gaagctcctc
     1501 gccgaggcca aggtgctcgc caaggaatac ggccgccgtc ccgaggagtg ggaggtgcag
     1561 atgctcctcg gcgtgcggag caatcttcaa gacgagctgg ttcgcgaggg ataccggatg
     1621 cgggtctacg tcacgtacgg caccgaatgg gtcccatact tcatgcggcg cctggccgag
     1681 cgtcccgcca acgtgggctt cgtgctccga agcctgatga aggggcgcta gcccgcttcg
     1741 ttccgttcgg cccgctcggc caactccgcg agattcgcca ccgcgaggtc gggtgacccc
     1801 ggccgtgcat gcgggcgcgc cacgtacacg ctggtgaggc cgagcccgcg cgccgtggcc
     1861 aggtcgtaat cggcgtacgc cgagacgtgc caccaccatg ggcccggcgc caccccgagc
     1921 cgcgacgacg cggcgtgcca catccgcgcg tccggcttgt agacgccgac gtcctccgcg
     1981 cagatccacg cgtcgagccg gaatccgagc gcggcctgca cctgctcgcc gtgctcacgg
     2041 tcgctgttcg tggtcgcggc gcaaggcgcg accgcgcgca ggcgccggag ggcgtcccgc
     2101 gaatcggggt agagcggcca ggttcccgcg tctctgccga tggcgcgcgc ggtctccgcg
     2161 tcgacgccca gcacgcgaac caggctctcg gcggtgatgt cggcgtagga tcgaaagggg
     2221 cccgactcca ggtccgcttg cgcgtcgatc agctggtcga acgcgtcgcc gtccaaggcg
     2281 acgcccaggc gctcgagcgc gtcggccagg ccgcggcgcc agtcgagcac cgtgccgaag
     2341 atgtcgaagg tgatgagtcg cggccgcatg gccgccagtc tgccacgctc cacgccgcgg
     2401 cgcagccctg aaggcgccgc cggaggaaga acgatgaggg aagccgaacg gatcgcggac
     2461 ctgcaccgcc gggctttcga cggcgactcc tggcatggac cccacgtctt cgagatcctg
     2521 gagggcatag acgcggcccg cgccgcgcgg aagccggtcg ccggggcgca cagcgtctgg
     2581 gaggtcgtgc ttcacatgag agcctgggag gatgtcgtgg tgcgtcggct tcgcggggag
     2641 atcgtcgagc tcaccgacgc ccaggactgg cctcgggtgg ccgatccatc cgcggccgcc
     2701 tggaaaacgg cgatcgtcgg gctgcgcgag acccacgacg agctcgcccg gacgatcgcc
     2761 gcgatcccgg acgagcggct ggagacgacg gcgccgggcg ggaggacgac gctctaccgg
     2821 ctggcccacg gcgccgtgca tcacgccctc tatcacgcgg gacagctcgc catcctgaaa
     2881 aaggggtaac ctctagagcg gcggcgggat cgggccggcc ccgaagtcgc gactccgtca
     2941 agaacggccc gctccctgcc gatccatc
//
LOCUS       DASSLZ010000286         9786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1103484, whole genome shotgun sequence.
ACCESSION   DASSLZ010000286 DASSLZ010000000
VERSION     DASSLZ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9786
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103484"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFM00_10860"
     CDS             complement(<1..671)
                     /locus_tag="VFM00_10860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7498790.1"
                     /translation="MSSTLPSGSRLGPYQIDELIGAGGMGEVYRARDTRLGRQVAVKV
                     LPKAFAADADRLKRFEREARSAGQLNHPNVLAIHDIGVETGVPYIVSELLAGTTLRAR
                     IEEGPIAPRKAIAMAIQIAKGLEVAHAKGIVHRDLKPENLMVMSGDHVKIVDFGLAKL
                     TQAESASDGEGTSPLQSQLTGTGAIMGTASYMAPEQIRQQPVDHRADIFSVGAILYEM
                     LTAQRA"
     gene            complement(668..3202)
                     /locus_tag="VFM00_10865"
     CDS             complement(668..3202)
                     /locus_tag="VFM00_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7498791.1"
                     /translation="MPNTITAGTRLGPYEIHELIGRGGMGEVYRAKDGRLGRQVAVKV
                     LPTSFAADADRLKRFEREARSAGQLNHPNVVAIYDIGTTGDVPYIVSELLDGTDLRAR
                     LSKGAIPPRKAVAMGIQIAKGLAAAHAKGIAHRDLKPENLMVMSGDHLKIVDFGLAKL
                     THADAAADGDVTGVLATQLTGTGTIMGTASYMAPEQIRQQPVDHRADIFSVGAILYEM
                     LTAQRAFEGPTPADRISAILHNTPTELPADIEDAAPGLPALIEHCLEKNVEDRFQCAH
                     DLVFALQQVEAAASSRRGATATAGATGAAAGVPGAIGVLDLKRHTFRRTTYREGTILS
                     ARFASDGQGICYGAAWEGRPAELFWAYPGNPESRALGFPRTDVLAISVTGEMAVSLRR
                     HPRGGFIYSGMLARMPAGGGAPREILDNVYEADWSPDGRQLAIVREEAGMTRVEYPIG
                     NVLYKTAGWVSHMRVAPDGKHVAFIDHPFRGNDAGGPAIVDMSGTSKSLSPGWSSARG
                     LAWSPDGKEIVFTAFRTGVGRSLYRVTPEGVERPILEVPGHMTLQDISKQGAALVALE
                     NERMRMQYARHAHATATDLTWLDWTLVRDLSSDGSRILFDETGVGGGELHAVYLRGTD
                     GSPAIRLGDGIGFTFSPDGQWALGTAGNVSHGLTLLPCGAGEGRFLELQGLEVIYGTW
                     FPDGMSFCALAHEPGKGARLFRVDPVTGKYEAFSEEGLTSFDPLVSPDGRLVSCQGPD
                     RRIKIYPVDGGEPRPLPGVLPNERMIRWSDDGSAGFVFGRGELPAKIFKIDIATGERT
                     LWKELSPPDPTGVEGITSIRMTPVGDAYGYSYAQRLNDLYVVEGLF"
     gene            complement(3415..3978)
                     /locus_tag="VFM00_10870"
     CDS             complement(3415..3978)
                     /locus_tag="VFM00_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7498792.1"
                     /translation="MELNHRFILTGAPGGGKTAILARLGPGIRPVAEPAREVLAEERA
                     AGGRGTPDQDAGRFVELLLARSIEKYEAAGSGEPVLYDRGIPDCVGYAVHLGADPSPG
                     VEAAVRYRYHPQVLVLEPWEAIYTTDDERKMTYAQAVGFHEELLEGYRNAGYELITVP
                     REPIEQRIAFIREFIARATLREPRTAD"
     gene            complement(4169..5380)
                     /locus_tag="VFM00_10875"
     CDS             complement(4169..5380)
                     /locus_tag="VFM00_10875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020415764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7498793.1"
                     /translation="MSYDVIVVGARCAGSPTAMLLARKGYKVLVVDKAAFPSDTLSTH
                     MVHPPGVAALKRWGLLDRVAATGCPPITRYKFDFGRVVLEGNPGTSEMPVSYGPRRTV
                     LDKILVEGAAEAGVDVREEFTVDEILIENGRVTGIRGHGKNGASVTERAKVTVGADGR
                     FSKVAEAVKPEAYNEAPPILAGYYSYWSNLPTEGRFENYALPHRGCALFGTNDGLTLL
                     VGGWPMTEFETNKKDAEANWMKMFDMHPALAERIRAAKRETKLFGTYVPNYFRKPYGD
                     GWALVGDAGYNRDFITAMGITDAFLSADLCAEALDRSLGGAEPYNETMAKYQYQRDER
                     VTPIFHFTCQLATCEPPPPQVQQIFGAMAGNQEQMDAFARMNAACLSPAEFFSEENVG
                     KIFGAASTRQL"
     gene            5583..6065
                     /locus_tag="VFM00_10880"
     CDS             5583..6065
                     /locus_tag="VFM00_10880"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7498794.1"
                     /translation="MSKVALVLVGFLILAAVLIAATPPPANPRAEVTAAVNAYIDAEN
                     RGDVYEMMALVSRRDDIVSISDGQIQRGWQTIRNSNDQIVGHESGPGMTLGPVDVMVL
                     GPTAAVAVAPFTFTVASAQGSVRVPGATSFVFEKSSGKWLVVHEHTSITTQEVARGIE
                     "
     gene            complement(6259..8784)
                     /locus_tag="VFM00_10885"
     CDS             complement(6259..8784)
                     /locus_tag="VFM00_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7498795.1"
                     /translation="MTETIPPGSRLGPYEIQGLLGAGGMGEVYRAKDARLGRDVAVKI
                     LPSSFAADAERQRRFEREARSAAQLNHPNIVAIHDVGVAGAVPYLVSELIEGTTLRAR
                     VAQSPLPPKKAIAFAIQIANGLAAAHARGIVHRDLKPENLMVLKGDHLKIVDFGLAKL
                     MSGEAGAGAAATREDDTASIAPQLTETGAILGTASYMAPEQIRQQTVDHRADLFAVGA
                     ILYEMLTARRAFDGPTPMDRLSAILNTKPEELPVSIEEAAPGISSLVERALEKAPEDR
                     FQTAHDLAFALSLIETASRRSGAARPDVLPGAESTPAPMTALTYKRITYRQGSLLSAR
                     FAPDGQAVCFCASWEGRPAELFWAYPGNPESRALGFPGTDILSIARSGEMAVSLRRRA
                     RGGFIYSGMLARMPSGGGAPKEILDNVREADWTPDGRQLVILRDEGGFERIEYPAGKV
                     LHRTAGWISHMRLSPDGTKIAFLDHPLRGNDAGGPAMVDLSGRYTSLSPGWASTRGLA
                     WSPDGKEILFSAFRTGVGRELYAISPEGTMRLLLQVPGHMTLNDVSREGAVLFTLENE
                     RMRTDFLTPGETVPRDLSWLDWSMPRGMTPDGRELLLEESGVGGGELHSVYLRGTDGS
                     PAIHVGDGQGFDLSPDGQWVVAGIGRSPSVLTLLPAGAGEPKTIPTGAWDVNSARWFP
                     DGTICMIAHEPGHGLRLHRLDPATGKFEPFSEEGLSWHEVMVSPDGRSVAAMGPERTL
                     KIYFTDGAEPRGLPQVTLADRPVGWGADGAALFVFTRGELPARVFQVDHATGERTEWK
                     RLSPLDPAGVEGVTAVRMSADGQAFAYSYMQRLSTLYVLEGLL"
     gene            9470..>9786
                     /locus_tag="VFM00_10890"
     CDS             9470..>9786
                     /locus_tag="VFM00_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HET7498796.1"
                     /translation="MSLPRRGLAAPLLILAATFVAAIALVPTTPAQAAPLFQADFESY
                     DAGATQAFLPGAAQSQPTGVAAGDMNGDGLADLVVACPGSPAGAFTGSTISVLLRRTS
                     AAVP"
BASE COUNT         1475 a         3238 c         3420 g         1653 t
ORIGIN      
        1 gcgcgctgcg cggtgagcat ctcgtagagg atcgccccga ccgagaagat gtcggcgcga
       61 tgatccaccg gctgctggcg gatctgctcg ggcgccatgt agctcgccgt gcccatgatg
      121 gcgccggtgc cggtgagctg cgactggagc ggggacgtgc cctcgccgtc cgacgcgctt
      181 tccgcctgcg tcagcttggc caggccgaag tcgacgatct tcacgtgatc gccgctcatc
      241 accatgaggt tctcgggctt aaggtcgcgg tgcacgatcc ccttggcgtg cgccacctcc
      301 agccccttcg cgatctggat cgccatcgcg atcgccttgc gcggcgcgat cggcccctcc
      361 tcgatgcgcg cgcggagcgt ggtccccgcg agcagctcgc tcacgatgta ggggacgccc
      421 gtctcgacgc cgatgtcgtg gatggcgagg acgttcggat ggttcaactg tcccgccgag
      481 cgcgcctcgc gctcgaagcg cttcaggcgg tcggcgtcgg cggcgaacgc cttggggagc
      541 accttgaccg cgacctggcg gcccagccgc gtgtcgcgcg cgcggtagac ctcgcccatg
      601 ccccccgcgc cgatcagctc gtcgatctgg taggggccga gacgcgatcc ggagggaagc
      661 gtgctgctca aaacagcccc tcgaccacgt agaggtcgtt cagccgctgc gcgtagctgt
      721 agccgtaggc gtcgcccacg ggggtcatgc ggatcgacgt gatcccctcg acgccggtcg
      781 ggtcgggcgg cgagagctcc ttccagagcg tgcgctcgcc ggtggcgatg tcgatcttga
      841 agatctttgc gggcagctcg ccccgtccaa agacgaagcc ggcggaaccg tcgtcgctcc
      901 agcggatcat ccgctcgttg ggaaggacgc cgggcagcgg cctgggctcg cctccgtcga
      961 ccgggtagat cttgatccga cggtcgggtc cctgacagga gaccaggcgg ccgtccggag
     1021 agaccagcgg gtcgaatgac gtgagcccct cctcgctgaa cgcctcgtat ttgccggtca
     1081 cgggatcgac gcggaagagc cgcgcgccct tgccgggctc gtgggcgagc gcgcagaagg
     1141 acatcccgtc ggggaaccag gtgccgtaga tcacttccag cccttgcagc tcgaggaacc
     1201 gcccctcgcc ggcgccgcag ggaagcagcg tgagtccgtg cgacacgttg cccgcggttc
     1261 cgagcgccca ctggccgtcg ggcgagaagg tgaagccgat gccgtcgccc agacggatcg
     1321 ccggagagcc gtcggtgccg cgcaggtaga ccgcgtgcag ctcgccgccg ccgacgccgg
     1381 tctcgtcgaa gaggatgcgg gatccgtcgc tcgagagatc gcgcaccaac gtccagtcca
     1441 gccacgtcag gtcggtggcg gtcgcgtgcg cgtgccgggc gtactgcatc cgcatccgct
     1501 cgttctcgag cgcgacgagg gcggcgccct gcttggagat gtcttgcagg gtcatgtggc
     1561 cgggcacttc gaggatcggc cgctccacgc cttcgggcgt gacgcggtag agcgagcggc
     1621 cgacgccggt gcggaaggcg gtgaagacga tctccttgcc gtcgggagac caggccagcc
     1681 cgcgcgcgct ggaccagccg ggcgacaggc tcttcgacgt cccgctcatg tccacgatcg
     1741 cggggccgcc ggcgtcgttg ccgcggaagg gatgatcgat gaacgccacg tgcttgccgt
     1801 cgggcgcgac gcgcatgtgg ctgacccaac ccgccgtctt gtagagcacg ttgccgatcg
     1861 ggtattcgac gcgcgtcatg cccgcctcct cgcgcacgat ggcgagctgg cggccgtcgg
     1921 gactccagtc ggcctcgtag acgttgtcga ggatctcgcg cggggcgccg ccgcccgcgg
     1981 gcatgcgcgc cagcatgccg ctgtagatga acccgccgcg cggatggcgt cgcaacgaca
     2041 ccgccatctc gccggtgacg gagatggcga gcacgtcggt gcgcgggaag ccgagcgcgc
     2101 gcgactcggg gttcccggga tacgcccaga acagctcggc gggccgtccc tcccaggccg
     2161 cgccgtagca gatcccctgg ccgtcggaag cgaagcgggc ggagaggatg gtgccttcgc
     2221 ggtaggtggt gcggcggaag gtgtggcgct tcaggtcgag aacgccgatg gcgccgggaa
     2281 cgcctgccgc ggcgccggtc gcgccggctg tggcggtcgc gccgcggcgc gaggatgcgg
     2341 ccgcctccac ctgctgcagt gcgaacacca ggtcgtgcgc gcactggaac cgatcctcga
     2401 cgttcttctc caggcagtgc tcgatcaggg ccgggagccc gggtgcggcg tcttcgatgt
     2461 cggcgggcag ctccgtgggc gtgttgtgca ggatcgcgct gatccggtcg gccggcgtcg
     2521 gcccttcgaa cgcgcgctgc gcggtgagca tctcgtagag gatcgccccg accgagaaga
     2581 tatcggcgcg atgatccacc ggctgctggc ggatctgctc gggcgccatg tagctcgccg
     2641 tgcccatgat ggtgccggtg ccggtgagct gcgtggcgag aacgcccgtc acgtcgccgt
     2701 cggcggccgc gtcggcgtgg gtcagcttcg cgagcccgaa gtcgacgatc ttcagatgat
     2761 cgccgctcat caccatcagg ttctcgggct tcaggtcgcg gtgcgcgatc cccttcgcgt
     2821 gcgcggcggc gagccccttc gcgatctgga ttcccatcgc gaccgccttg cgcggcggga
     2881 tcgctccttt tgagaggcgc gcgcggaggt cggtgccgtc gagcaattcg ctcacgatgt
     2941 aggggacgtc gcccgtggtg ccgatgtcgt agatcgcgac cacgttcgga tgattgagct
     3001 gccccgccga gcgcgcctcg cgctcgaagc gcttcaagcg gtcggcgtcc gcggcgaacg
     3061 acgtggggag gactttgacg gcgacctggc ggccgaggcg tccatccttg gcgcgataca
     3121 cctcgcccat tccgccgcgt ccgatcagct catggatttc gtaggggccg aggcgcgtcc
     3181 cggccgtgat ggtgttgggc aatggggtag ctccgagcaa ggcgttcgct tcgagatttc
     3241 gtgggattag tagcggttaa gtatagcagg tttatgtgtt ttggcgggag gcgcgcgcgg
     3301 gcggggcgtc tcggcggggc cggctcgagt gcgcggtggg ttaatattta tattaactta
     3361 cgccagcgca agtgctggct cgcgtcaact tgcgccagct caagttgatc tcacttagtc
     3421 tgccgtgcgg ggttcacgga gggtggccct ggcgatgaac tcccggatga aggcgattcg
     3481 ttgctcgatg ggttcgcggg gcacggtgat cagctcatag cccgcgttcc ggtatccctc
     3541 gagaagctcc tcgtggaagc cgaccgcctg cgcgtaggtc atcttccgct catcgtcggt
     3601 ggtgtaaatc gcttcccagg gctcgagaac gaggacctgg gggtgatagc ggtaacgcac
     3661 ggcggcttcg acgccggggg aagggtcggc gcccaggtgg acggcgtagc cgacacagtc
     3721 cgggatgccg cgatcgtaga gcaccggctc gccagagccg gccgcttcgt atttctcgat
     3781 cgaacgggcg aggagaagct ccacgaaccg cccggcatcc tgatcgggcg tccctcgtcc
     3841 gccggcggct cgctcctcgg caaggacctc gcgcgcgggc tcggccacgg ggcggatgcc
     3901 cggcccgagc cgcgcgagaa tcgcagtctt cccgccgccg ggcgcgccgg taaggatgaa
     3961 gcggtggttc agctccatcg cgccagctgg gatgttgcag gcttcgggat gttcatcggg
     4021 agatgggcca aggtggcaca gtcaacatac tccagagtat gttggtcgca cgcctcaggg
     4081 ccagttactc acgtgaatta atataaatat taacttgcgc cggcgcaagt accgcatcgg
     4141 gcatggaccg gcaacccttc gcttccggct acagctgcct cgtgctggcc gcgccgaaga
     4201 tctttcccac gttctcttcc gagaagaact ccgcgggcga gaggcaggcg gcgttcattc
     4261 tcgcgaaggc gtccatctgc tcctggtttc cggccatcgc gccgaagatc tgctgcacct
     4321 gcggcggggg cggctcgcac gtggcgagct ggcaggtgaa gtggaagatg ggcgtgacgc
     4381 gctcgtcgcg ctggtactgg tattttgcca tggtctcgtt gtagggctcg gcgccgccca
     4441 gtgagcggtc gagcgcctcg gcgcacaggt cggcgctcag gaaggcatcg gtgattccca
     4501 tcgcggtgat gaagtcgcgg ttgtagccgg cgtcgcccac cagggcccag ccgtcgccgt
     4561 agggcttccg gaaatagttc ggaacgtagg tgccgaagag cttggtctcg cgcttggccg
     4621 cgcggatgcg ctcggccagg gccggatgca tgtcgaacat cttcatccag ttcgcctcgg
     4681 cgtccttctt gttcgtctcg aactcggtca tgggccagcc gccgaccaat agcgtgagcc
     4741 cgtcgttggt gccgaagagc gcgcagccgc ggtgggggag ggcgtagttc tcgaaccgtc
     4801 cctccgtggg cagattgctc cagtacgagt agtagccggc caggatcggc ggcgcctcgt
     4861 tgtaggcctc gggcttcacg gcctcggcga ccttggagaa gcggccgtcg gcgccgaccg
     4921 tgaccttggc gcgctccgtc accgaagcgc cgttcttgcc gtggccccgg atccccgtca
     4981 cgcggccgtt ctcgatgaga atctcgtcga cggtgaattc ctcccgcacg tcgacgccgg
     5041 cttccgccgc cccttcgacc aggatcttgt ccagcacggt gcgccgcgga ccgtacgata
     5101 ccggcatctc cgacgtgccg gggttcccct cgagcaccac gcgcccgaag tcgaacttat
     5161 agcgggtgat cggcggacag ccggtggccg cgacccgatc gagcagcccc cagcgcttca
     5221 gcgcggcaac tccgggcgga tggaccatgt gggtcgagag ggtgtcgctg gggaaggccg
     5281 ccttgtcgac gaccaggacc ttgtagccct tccgggcgag gagcatcgcg gtgggggagc
     5341 cggcgcatcg cgcgccgacg acgatgacgt cgtagctcat gaggacctcc ttaggggtcg
     5401 cgctgccccc ccccgtgctg gcgaagaagc gcggacggcg attctagcac tcttcagggt
     5461 ccacgcaatt gaccgtatcc tggctctgtg ctgtagctta cgcgaaatat ttcacctgag
     5521 ttgttgactc cacccccgcc caggcctagc gtggtcgagc cttcccaggc aaggaggtcc
     5581 ccatgtccaa ggttgctctg gtcctggtcg gattcctgat cctcgcggcg gtcttgatcg
     5641 ccgccactcc accgcccgcc aacccccgcg ccgaagtcac ggctgccgtc aacgcgtaca
     5701 tcgatgccga gaaccgcggc gacgtctacg agatgatggc cctggtgagc cggcgcgacg
     5761 acatcgtctc catcagcgac gggcagatcc agcgcggctg gcagacgatc cgaaacagca
     5821 acgatcagat cgtcggccac gagtccgggc ccggcatgac gctggggccg gtcgacgtga
     5881 tggtcctggg cccgacggcc gccgtggcgg tcgcgccctt cacgttcacg gtggcctcgg
     5941 cgcaggggtc ggtccgggtc ccgggcgcga cctccttcgt gttcgagaag agtagcggca
     6001 agtggctcgt ggtgcacgag cacacgagca tcacgacgca ggaggtcgcg cgcgggatcg
     6061 agtgatggaa gtcggggcgc gtcccgcccc cgaaggcggg gcggccacaa ccgcccccga
     6121 agatgccagc gccggtgagg cgcaaacgag gcggagcctg caattgaatg cgggccccgc
     6181 ctcaggcgtg tttgggatct catgcgattg cagatggtcc gggcgaactg gatcttgagc
     6241 cgctgattag ccgatgtcct aaaggagccc ctccagtacg tagagcgtgc tcagccgctg
     6301 catgtagctg tacgcgaagg cctggccgtc ggctgacatt cgaacggcgg tcaccccctc
     6361 gacgcccgcg ggatccagcg gcgagagccg cttccactcg gttcgttccc cggtggcgtg
     6421 gtcgacttgg aagacgcgag ccggcaattc gccgcgggtg aacacgaaga gcgccgcccc
     6481 atccgcgccc cagcccacgg ggcgatcggc cagcgtgacc tgcggcaggc ctcgtggctc
     6541 ggcgccgtcc gtgaagtaaa tcttcagggt ccgctcggga cccatggcgg cgacggatcg
     6601 tccatccggc gacaccatca cttcgtgcca ggaaagtccc tcttcgctga acggctcgaa
     6661 cttcccggtg gcggggtcca gccggtgcag ccgcaagcca tggccgggct cgtgagcgat
     6721 catgcagatc gtgccgtcgg ggaaccagcg cgccgagttc acgtcccagg cgccggtcgg
     6781 aatcgtcttc ggctcgccgg cgcccgcggg gagcagtgtc agcacggagg gtgaccggcc
     6841 gatcccggcg accacccact gaccgtcggg ggaaaggtcg aacccctggc catcgcccac
     6901 gtggatcgcg ggagatccat ccgtcccgcg caggtacacc gagtgcagct cgcctccacc
     6961 cacgcccgat tcttcgagca gcaactcgcg cccgtccggc gtcatcccgc gcggcatcga
     7021 ccagtcgagc caggaaaggt cgcggggcac cgtctcgccg ggcgtgagga agtccgttcg
     7081 catccgctcg ttctcgaggg tgaagaggac cgccccttcc cgcgagacgt cgttcagcgt
     7141 catgtgcccc gggacctgca gcagcaggcg catcgttcct tcgggggaga tggcgtagag
     7201 ctcacggccg acgccggtgc ggaacgcgct gaagaggatc tccttgccgt cgggggacca
     7261 ggcgagtccg cgcgtgctgg cccatccagg ggacagtgag gtgtacctgc cggagagatc
     7321 caccatggcg gggccgccgg cgtcgttgcc gcgaagggga tggtcgagga acgcgatctt
     7381 cgttccatcc ggcgagagcc gcatgtggct gatccagccc gccgttcgat ggagcacctt
     7441 gccggctgga tactcgatcc gctcgaaacc gccctcatcg cgcaggatca cgagctgccg
     7501 gccgtcggga gtccagtcgg cctcgcgcac gttgtcgagg atctccttgg gcgcgccgcc
     7561 tcccgagggc atgcgcgcga gcatcccgct atagatgaag ccaccgcggg cccggcgccg
     7621 gagcgaaacc gccatctcgc ccgagcgcgc gatggagaga atgtccgtgc ccggaaatcc
     7681 aagggcgcgc gactccggat ttccgggata ggcccagaag agctccgcgg gccgcccctc
     7741 ccacgacgcg cagaagcaca ccgcctggcc gtcgggcgcg aagcgtgcgg aaagaaggga
     7801 tccctgccgg taggtgatgc gcttgtaggt cagggcggtc atcggggccg gggtggactc
     7861 tgcgcccggc agtacgtccg gccgcgcggc gccgctccgc cgggacgccg tctcgatcag
     7921 gctcagcgcg aacgcgaggt cgtgtgcggt ctggaaccgg tcctccggtg ccttctccag
     7981 ggcgcgctcc accagcgaag agattcccgg cgccgcttcc tcgatggaga cgggaagctc
     8041 ctccggcttc gtgttcagga tcgcgctcag gcggtccatc ggtgtggggc cgtcgaacgc
     8101 gcggcgtgct gtcagcatct cgtagaggat cgcgcccacc gcaaacaggt cggcccgatg
     8161 gtccacggtc tgctggcgga tctgctcggg ggccatgtag ctcgccgtgc cgaggatggc
     8221 gcccgtttcg gtcagctgcg gcgcgatgga cgcggtgtcg tcttcgcgcg tggccgcggc
     8281 cccggccccg gcttcgccgg acatgagctt cgccaggccg aagtccacga tcttcaagtg
     8341 atcgcccttc agcaccatga ggttctcggg cttgaggtcg cggtggacga tccccctggc
     8401 gtgcgcggcc gcgaggccgt tcgcgatctg gatggcgaac gcgatcgcct tcttcggggg
     8461 aagcgggctc tgcgccacgc gggcgcgcag cgtcgtcccc tcgatcagct cgctcacgag
     8521 gtagggcacg gcgcccgcga cacccacgtc gtggatcgcg acgatgttcg gatggttgag
     8581 ctgcgcggcg gagcgggcct cgcgttcgaa gcgacgctgg cgctcggcgt cggccgcgaa
     8641 ggaagagggg aggatcttga ccgcgacatc ccggccgagc cgtgcgtcct tggcgcggta
     8701 cacctcgccc atgccgcccg cgcccagcaa tccttggatc tcgtagggcc cgagacggga
     8761 accgggcggg atggtttcgg tcatgtcaga gcctcggatg aatgctgggg atcggcaccc
     8821 tcagaacagt aaacgaatcg acggagcgat cgtaacggga gtgtggaaca tactctggag
     8881 tatgcgtttg agcctaaaag gcacattcca tccccgttca gcctgggccg tcagagcggc
     8941 ctgacaagtt ggcagtatct gcggcaaatg gtggctccga gccgtcattc ggcgcttcgc
     9001 gaacaggcag cgaatccttg gcttacggat cccgcccttc gggcggggta tgggtacgaa
     9061 ccctgcagga gatgacgatg tcgggagatt tgggattgcc gacccaacag gcccagcctg
     9121 agggaggtga ccatgaaccg gcttatgaat gtttcaccgg tgcccggcac gtttggcttt
     9181 gtgacgttcc tggtgctgtt gcccgtggcg ggattggtct ttggaatcct gctcctggca
     9241 ttgctcggcg ccatcttcca ttaagaggag aggaacgcac tcccctggca gggggcccaa
     9301 cgtaacgcaa caccggtgag tttgaacggc ccgccgattc gatgttcggt ctctcctgct
     9361 cggacgtagc catggagcag gggggagagt cggcgggctc gttctacacc cgcgcctagc
     9421 cctccggaag cacgtccatc gcggccccga cccttcccgg agagcatcca tgagcctgcc
     9481 gcgccgcggc ctcgccgcac ctcttctgat cctggcggcg accttcgtgg ccgccatcgc
     9541 cctcgtcccg acgacccccg cccaggcggc tcctcttttc caggcggact tcgagtccta
     9601 cgacgcgggc gccacgcagg cgttcctgcc gggcgccgcc cagtcgcagc cgaccggcgt
     9661 cgcggcgggc gacatgaacg gggacggcct ggccgatctc gtggtcgcct gtcctggaag
     9721 tcccgcgggc gccttcacgg ggagcacgat atcggtcctt cttcgccgga cctctgcggc
     9781 cgtgcc
//
LOCUS       DASSLZ010000287         7927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1624827, whole genome shotgun sequence.
ACCESSION   DASSLZ010000287 DASSLZ010000000
VERSION     DASSLZ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7927
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624827"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFM00_10895"
     CDS             <1..279
                     /locus_tag="VFM00_10895"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET7498797.1"
                     /translation="LEHTSLRYRNEGRAGATKAIGKLGIRTDAAEARLVELLGDRWFR
                     VRNAAAWSLWKIKSPRAEAAIAAALEREALDMCRGAMRDALDGVRKGR"
     gene            329..814
                     /locus_tag="VFM00_10900"
     CDS             329..814
                     /locus_tag="VFM00_10900"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7498798.1"
                     /translation="MPLQTGTKLGPYEIVAPLGAGGMGEVYRARDPRLGREVAVKVLP
                     PAFARDRERLQRFEHEARAAGALNHPGITAIFDLGTQDGIPFLVSELLEGESLRTVLA
                     NGPLPPARVADLGIQAAQALAAAHAKGIVHRDLKPENLHLLPESLCRRPSTPRFPASK
                     R"
     gene            811..2535
                     /locus_tag="VFM00_10905"
     CDS             811..2535
                     /locus_tag="VFM00_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498799.1"
                     /translation="MIRRCLEKRVDDRFGTTRDLAFTLRLLTDVQERAVPSAPKGSAA
                     ALPPPRDVKFRQLTFREGLVSGARFASDGRTVVYAATYAGEQPDLYLARVGSHEHRPL
                     HMPGARILSISRADELLVRLRSRDVGGFVNLGVIARVPLMGGIPREVADEVFQAVFSP
                     DGRQIAAIRFVSGAARLEYPLGTVLAETEGWFSSPAALADGEVFYVDHPTRGDNAGYP
                     MVAGPRGQRRLSDERFSSVAGGAAFPDRRHVLMSGQQADGSGGLFFVSLEGGYRPAYR
                     TPSWAFVEDVSDAGDVLLTSRTPRMLMETGTRGHEPPRDLSWLDWTLARDLTADGTTV
                     LFDETGIGAGQATVFVRGTDGSPPVRIAEGDGVGLSPDGRTAIVIDPADPTGFELVPI
                     GIGSAESHRTAPVRVMTGAWFPDGKKLCIGGHEPGKRPRLYRYDLATRAIEPLTEEGT
                     GIGYCSVSPDGAFVFVLGPRGHSIYPVGGGEPQVLSMIGTQHRGIGWSSDGRAVFAFE
                     RGRIPAPVLRIDLASGRAEPWMEVHPRSTGGVNGFNVVWLSADGERYVGGYSRTLSEL
                     FLLKGLAP"
     gene            2532..5048
                     /locus_tag="VFM00_10910"
     CDS             2532..5048
                     /locus_tag="VFM00_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7498800.1"
                     /translation="MSGTARLAPGTRLGPYEIVAPLGAGGMGEVYRARDPRLGREVAV
                     KVLPRAFASDPDRQRRFEAEARAAGALNHPNIVTLFDVGATDGTPYLVTEVLEGETLR
                     ALLSRGPLAPARAAALLGQAALGLAAAHAKGIVHRDLKPENLFVLPDGRVKVLDFGIA
                     KLVAGEASAAAETTPVFASMTASGTILGTVSYMAPEQLRDQTVDHRADLFALGAILYE
                     LVSGRLAFPADTAADRVTAILTADPKTLDPAVEAAMPGLSELIRRCLEKRPETRFESA
                     RDLAYALEILAGASGSSRQPMEAPVGTPTAGLSLKIRQLTFREGYLSEARFAPDGVTV
                     VYRAAWEGQPPRIFLSRTDNPDYQPLQLGNAELLAVSSTAELAVGLNPRDMGGFIQLH
                     TLARVSMLGGVPRELAKDVFMADWGPGGRSLAAIRLVDGRFRLEYPLGTVVHETSGWF
                     SRIRVAPSGKQIAFFEHPVTGDNAGDLKIVEPGRPTRTLVTGLNSAGTLAWRPDGSEI
                     WYSATAEGEGAGLIYAVTLEGRVRVVYQTLGWPVLYDFSQSGDALIGRMKPRLRLQTG
                     TRSAAGADTVELSWLDWSLARDLTEDGSMALFDETGPGVMHGAAVYVRSTDGSAAIRI
                     GEGVGMGLMPDGLHALTIEVIDSERVVGICPLGAGEPRRIRLGGVVTHFGRPFPDGRT
                     AVIVGHEPGQGGGLYRLDLETGGLERITREPITVRHARPTPDGSAVLASRADGTYALF
                     PVDGGPSRAVPGLESDERPSQFTEDGRHVYTFRRGVVPCPVARVDVETGRREPWFEIE
                     PVTRNGTDSFISVLFTPDGERYIASFGQYVNDLFLVSDLR"
     gene            5086..7050
                     /locus_tag="VFM00_10915"
     CDS             5086..7050
                     /locus_tag="VFM00_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET7498801.1"
                     /translation="MHLTTVTRTIRGKALILWDPKRPGKKRDAIDTDQITPAEDCVSE
                     SLATLDERWKAGAFRHVMPDFRARVHGGETFLIAGDRFAIGSSREMSPAGLKGIAEEA
                     GLEMVVVCGANMGDIFRRNALNLGLHVVQSLEAVEDAADGDELEFDVETRALRNVTKG
                     KEYQPKALTPKEDEIRKSGGIFAMGRREVAPARGRVPAVEFPDPITASRMTATEQILW
                     AHRVDKSADVRPGATIQAYADLLPASDGTAPFAIHTFRQIVGDAAAVPRLAAIANDHF
                     VFTGKDADQKQTGISREFAAHHGIKAPYYAPPGTGIFHFYFPEQGLVIPGGFYPGADS
                     HSRAYGAYGAVGIGVGSTTLGFGWATGYVYVTVAKQRRVALRGRLQPWVSGKDIVLKL
                     LSGWGVKQSQGMSVEFQDEGGQLPIPYRNTIANMMAEAEAMNGIFPQDSVTDEWFQDR
                     GFKLRYPRVAPGEDAVYEMDETLDLTGVAPMIAKPFSPGNAYPAEEVATERLTFQKAM
                     IGSCTNGGYDDLLSAALVLRGARAMGIDRVKGKHEFIVFPGSGTVKLQIENPDPRLGG
                     ESVAQVLRAAGALIRESWCGPCFGQGPDALESGERAITSFNRNWQNRMGVGGEGYLAS
                     PAVVASSALAGYMAPPSELGLVWDAEIFGV"
     gene            complement(7057..>7927)
                     /locus_tag="VFM00_10920"
     CDS             complement(7057..>7927)
                     /locus_tag="VFM00_10920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7498802.1"
                     /translation="NPATALIAQDGVHHIEAGTSMSSPHVTGSVAILLAQAAWAGKSP
                     SAIKARLRSTARSDAFTGTVPNNVWGYGKLDIQAAAAPLFLATVTHPAKGAQIPPGKP
                     DSVTVVVGGATADSVEFDLSTDGGATYPVRLGTLYTVIPGPPRSLSYFVEASYSTLQA
                     KVRATAHAGVTTYVASSDSLFLISTPAAVEVDASSPRPRFALAANSPNPFNPVTTIRF
                     ELAESGRTSLRVFSAQGRMVRTLVNERLAAGGYRVRWDGKDDGGHGVASGIYFYELNE
                     GTRSLTRKMSLLK"
BASE COUNT         1164 a         2742 c         2836 g         1185 t
ORIGIN      
        1 ctggagcaca cctcgcttcg ctaccggaac gaggggcgcg ccggcgccac caaggcgatc
       61 ggcaagctcg ggatccgcac cgacgccgcc gaggcgcgcc tggtcgagct gctcggcgac
      121 cgctggttcc gcgtccggaa cgccgccgcc tggtcgctct ggaagatcaa gtcgccgaga
      181 gctgaggccg cgatcgccgc cgcgctggag cgcgaggcgc tcgacatgtg ccgcggggcc
      241 atgcgcgacg ccctcgacgg ggtgcgcaag gggcggtagg ctctccgggt tccatcgcgc
      301 gcaccgatcc ctccgggaat ccaaacgcat gccgcttcag accggaacca agcttggccc
      361 ctacgagatc gtcgcgcccc tgggcgccgg cggcatgggg gaggtctatc gcgcgcggga
      421 tccccgtctc gggcgcgaag tggcggtcaa ggtgctcccc cccgcattcg cgcgggaccg
      481 ggagcggctg cagcgtttcg agcacgaggc tcgggccgcc ggcgcgctga atcacccagg
      541 catcaccgcc atcttcgacc tcggcaccca ggatggaatc ccgttcctgg tcagcgagct
      601 gctcgagggg gagagcctcc ggaccgttct cgcgaacggc ccgcttccgc ccgcgcgcgt
      661 cgccgacctg ggaatccagg ccgcgcaggc gctcgccgcc gcgcacgcga agggtatcgt
      721 ccatcgggac ctgaaaccgg agaacctcca cctcctgccg gaaagcctct gccggcgacc
      781 ctcgacgccg cgattcccgg cctcgaagcg gtgatccggc gctgcctcga gaagcgcgtg
      841 gacgaccggt tcggcaccac gcgggatctc gcgttcacgc tgcgcctcct gacggacgtc
      901 caggaacggg cggttccttc ggctccaaag gggagcgcgg ccgcgctgcc gccgccgcgg
      961 gacgtgaagt ttcgccagct caccttccgg gaggggctcg tctcgggagc gcgcttcgct
     1021 tccgacgggc gcacggtcgt gtatgccgct acctacgccg gcgagcagcc ggacctctat
     1081 ctcgcgcgcg tgggaagcca cgagcatcgt ccgctgcaca tgccgggcgc gcgcatcctc
     1141 tcgatctccc gcgccgacga gctcctggtc cggcttcgtt cccgcgacgt cggaggcttc
     1201 gtgaacctgg gcgtcatcgc ccgcgtgccg ctcatgggcg gcatcccgcg ggaggtcgcc
     1261 gacgaggtct tccaggccgt gttcagcccc gatgggcgcc agatcgccgc gatccgcttc
     1321 gtctcgggag ccgcacgcct cgaatacccg ctcgggaccg tcctggcgga gacggagggg
     1381 tggttcagca gtccggcggc cctcgcggat ggagaggtct tctacgtcga tcatcccacg
     1441 cgcggggaca acgccggcta cccgatggtc gccgggccgc gcggtcagcg gcgcctgagc
     1501 gacgaacgat tcagctcggt cgccggaggc gcggcgtttc cggaccgcag gcacgtgctg
     1561 atgtcgggcc agcaggcgga cggcagcggc ggcctcttct tcgtatcact cgagggcggg
     1621 taccgccccg cctaccgcac cccctcctgg gcgttcgtgg aggacgtctc cgacgcgggc
     1681 gacgtcctcc tgaccagccg gaccccgcgc atgctgatgg agacgggaac gcgcggtcac
     1741 gaaccgccgc gcgacctctc ctggctggac tggacgctgg cgcgtgatct cacggccgat
     1801 ggaacgaccg tgctgttcga cgagacgggg attggggccg gccaggcgac cgtcttcgtt
     1861 cgcggaaccg acggttcccc gccggttcgc atcgcggagg gggatggcgt cgggctgtcg
     1921 ccggacgggc gcaccgcgat cgtcatcgac ccggccgatc caaccggctt cgagctggtg
     1981 ccgatcggaa tcggcagcgc ggaatcgcat cgcacggcac cggtccgcgt catgacgggc
     2041 gcgtggtttc ccgacgggaa gaagctctgc atcggcgggc acgaaccggg aaagcgcccc
     2101 cgcctctatc ggtacgacct ggcgacgcga gccatcgagc cgctgaccga ggagggaacg
     2161 ggcatcggct actgctccgt ctcgccggat ggcgcgttcg tattcgttct gggcccccgg
     2221 ggacattcga tctaccccgt tggcggcggc gagccgcagg tcctgagcat gatcggaacc
     2281 cagcaccgcg gcatcggctg gagctccgac ggccgcgcgg tcttcgcgtt cgagcggggg
     2341 cggattcccg ccccggtgct gcgcatcgac ctcgcgagcg gccgcgccga gccatggatg
     2401 gaagtccatc ctcgcagtac cggcggcgtg aacggattca acgtggtgtg gctctccgcg
     2461 gatggggagc gctacgtggg cggctactcg cgcacgctgt cggagctgtt tctcctgaag
     2521 gggctggccc catgagcgga acggcgaggc tcgcgcccgg gacgcggctc ggtccctacg
     2581 agatcgtcgc gcccttgggc gcgggcggca tgggggaggt ctatcgggcg cgcgatccgc
     2641 ggctgggacg cgaagtggcg gtcaaggtgc tcccgcgcgc gttcgcctcc gaccccgacc
     2701 ggcagcgacg cttcgaggcg gaggcccgcg cggccggcgc gctgaatcat ccgaacatcg
     2761 tcaccctctt cgacgtcggc gcgacggatg gaacgcccta cctcgtcacc gaagtgctcg
     2821 aaggggagac gttgcgcgcg ctcctttccc gcgggccgct ggcgcccgcc cgcgccgcgg
     2881 cgctcctggg ccaggccgca ctcggcctcg cggcggcgca cgcgaagggg atcgtgcatc
     2941 gggatctcaa gcccgagaac ctcttcgtcc tcccggacgg ccgcgtgaag gtcctcgact
     3001 tcgggatcgc gaagctcgtg gccggcgaag cgtcggccgc cgcggagacg acgccggtgt
     3061 tcgcctcgat gaccgcgtcc gggaccatcc tcggcaccgt ctcctacatg gcgcccgagc
     3121 agctccggga ccagacggtg gaccatcgcg ccgacctctt cgcgctgggg gcgatcctct
     3181 acgagctcgt atcggggcga ctggccttcc cggccgacac cgcggccgac cgcgtgacgg
     3241 cgatcctcac cgcggacccc aagacgctgg atccggccgt ggaggcggcg atgccgggcc
     3301 tgtccgagct gatccgccgc tgcctcgaga agcgtcccga gacccggttc gagtcggcgc
     3361 gcgacctggc ctacgcgctg gagatcctcg ccggcgcctc cggctcgtcg cggcagccga
     3421 tggaagcgcc ggtcgggacc ccgaccgcgg gcctgtcgct caagatccgc cagctcacct
     3481 tccgcgaggg ctatctctcg gaggcgcgat tcgcgcccga cggcgtgacg gtggtctacc
     3541 gcgccgcgtg ggaggggcag ccgccccgga tcttcctttc gcgaacggac aatccggact
     3601 atcagccgct tcagctcggc aacgccgagc tcctcgcggt ttcatcgacc gccgagctgg
     3661 ccgtcggtct caatccgcgg gacatgggcg gcttcatcca gctgcacacg ctcgcgcgcg
     3721 tgtccatgct ggggggagtg ccccgtgagc tggccaagga cgtcttcatg gccgactggg
     3781 gacccggcgg ccgatcgctc gccgcgatcc gcctcgtgga cgggcgcttc cgtctggaat
     3841 acccgctggg gaccgtggtg cacgagacgt ccggatggtt cagccggatt cgggtcgctc
     3901 cctccggaaa gcagatcgcc ttcttcgagc atccggtcac cggggacaac gcgggagacc
     3961 tgaagatcgt cgagccgggc cgccccacga ggaccctggt gaccgggctc aacagcgcgg
     4021 gcacgcttgc ctggaggccg gacggcagtg agatctggta ctcggccacc gcggagggag
     4081 agggggccgg gctcatctat gccgtgacgc tcgaggggcg cgtgcgggtg gtctaccaga
     4141 ccctgggctg gcccgtgctc tatgacttct cgcaatccgg cgacgccttg atcgggcgca
     4201 tgaagccgcg cttgagactc cagaccggca cgcgctcggc ggcaggcgcg gacacggtcg
     4261 agctctcctg gctcgactgg agcctggccc gggacctcac cgaggacggc tcgatggccc
     4321 tcttcgacga gaccggcccc ggagtcatgc acggcgcggc cgtttacgtt cggagcacgg
     4381 acggatcggc cgccatccgg atcggcgaag gggtcggaat gggcctcatg cccgacggcc
     4441 tgcatgcgct gacgatcgag gtcatcgact ccgagcgcgt cgtcgggatc tgcccgctgg
     4501 gagccgggga gccgcggcgc atccgtctcg ggggcgtcgt cactcatttc ggccggccgt
     4561 ttcccgacgg gcgtaccgcg gtgatcgtcg gccacgaacc cggtcagggg ggcggcctct
     4621 accggctcga cctggagacg gggggtctgg agcgcatcac gcgcgagccg atcacggtgc
     4681 gtcacgcacg gcccacgccc gacgggagcg ccgttctcgc gagccgggcg gacggcacgt
     4741 acgccctgtt tcccgtggac ggcgggccct cgcgggccgt ccccggcctc gagtcggacg
     4801 agcgcccctc ccagttcacg gaagacggaa ggcacgtcta caccttccgg cgcggcgtcg
     4861 tcccctgtcc ggtcgcgcgc gtcgatgtcg aaacggggcg gcgcgagccc tggttcgaga
     4921 tcgagccggt cacgcggaac gggaccgact cgttcatttc ggtcctgttc actccggacg
     4981 gggagcgata catcgcctcg ttcggccagt acgtcaacga cctgttcctc gtcagcgatc
     5041 ttcgctaggt ggcgcgcccg cgcggccccg cgctacactg accccatgca tctgaccacc
     5101 gtcacccgga cgatccgcgg caaggcgctc atcctctggg accccaagcg tcccgggaag
     5161 aagcgcgacg ccatcgatac cgatcagatc acgcccgccg aggactgcgt gtcggaaagc
     5221 ctcgccacgc tcgacgagcg ctggaaagcg ggcgccttcc gccacgtcat gcccgatttc
     5281 cgcgcgcggg tgcacggggg ggagacgttc ctgatcgcgg gcgaccgctt cgcgatcggt
     5341 tcctcgcgcg agatgtcgcc ggccgggctc aaggggatcg ccgaggaggc ggggctcgag
     5401 atggtcgtag tctgcggcgc gaacatggga gacatcttcc ggcgtaacgc gctcaacctg
     5461 ggcctgcacg tggtccagag cctggaagcg gtggaggacg cggccgacgg cgacgagctc
     5521 gagttcgacg tcgagacgcg cgcgctccgg aacgtgacga aggggaagga gtaccagccg
     5581 aaagcgctga ccccgaagga agacgagatc cggaagagcg gcggcatctt cgccatgggg
     5641 cggcgcgagg tcgcgccggc gcgcgggcgc gtgccggcgg tcgagtttcc cgatccgatc
     5701 accgcgagcc ggatgaccgc gaccgagcag atcctctggg cgcaccgcgt ggacaagagc
     5761 gcggacgtgc gcccgggcgc cacgatccag gcatacgccg atctccttcc cgcgtccgac
     5821 ggcaccgcgc ccttcgccat ccacaccttc cggcagatcg tgggggacgc ggccgcggtt
     5881 ccacgcctgg cggcgatcgc gaacgaccac ttcgtcttca cggggaagga cgccgatcag
     5941 aagcagaccg gcatctcgcg cgagttcgcc gcccaccacg gcatcaaggc tccgtactac
     6001 gcgccgccgg ggaccgggat ctttcatttc tatttcccgg aacaggggct cgtgattcca
     6061 ggtggtttct atccgggggc agactcccac agccgcgcct acggcgccta cggcgccgta
     6121 ggaattggcg tgggaagcac cacgctcggc ttcggctggg cgacgggcta cgtctacgtc
     6181 accgtggcga agcagcggcg cgtcgccctc cgcgggaggc tccagccctg ggtgagcggc
     6241 aaggacatcg tgctcaagct gctctccggt tggggcgtga agcagtcgca ggggatgagc
     6301 gtggagttcc aggacgaggg gggccagctc ccgattccct accgcaacac catcgccaac
     6361 atgatggccg aggcggaggc gatgaacggc atcttcccgc aagattccgt gaccgacgag
     6421 tggttccagg atcgcgggtt caagcttcgc tacccgaggg tcgcgcccgg ggaggacgcc
     6481 gtgtacgaga tggacgagac gctcgatctc accggcgtcg ccccgatgat tgccaagccg
     6541 ttcagtcccg gaaacgccta tcccgccgaa gaggtggcga ccgagcggct cacgttccag
     6601 aaagcgatga tcggaagctg caccaacggc ggctacgacg accttctctc ggccgcgctc
     6661 gtgctgcgcg gcgcgcgggc gatgggaatc gaccgcgtga aggggaagca cgagttcatc
     6721 gtctttccgg gaagcggcac ggtgaagctg cagatcgaaa atcccgatcc gcgcctgggc
     6781 ggggaatcgg tcgcgcaggt cttgcgcgcg gcgggtgctt tgattcgcga gtcgtggtgc
     6841 ggcccctgct tcggccaggg ccccgacgcg ctggaatcgg gggagcgcgc catcacctcg
     6901 ttcaatcgga actggcagaa ccggatgggc gtgggcggcg aaggctacct cgcgagcccg
     6961 gccgtggtcg cctcctcggc gctcgcgggg tacatggcgc ccccctcgga gctggggctg
     7021 gtgtgggacg cggagatctt tggggtctag tctcgcctac ttgaggaggc tcattttccg
     7081 ggtgagggag cgcgttccct cgttcaattc gtagaagtag atccccgacg cgacgccgtg
     7141 gccgccgtcg tccttgccgt cccatcggac ccggtagccg ccggccgcca gccgctcgtt
     7201 caccagcgtt cgaaccatcc gtccctgggc cgagaagacg cgcagcgacg tgcggccgct
     7261 ctcggccagc tcgaagcgga tcgtcgtgac cggattgaac gggttgggcg agttggcggc
     7321 gagcgcgaag cgtgggcgcg gcgaggacgc gtccacctcg accgccgcgg gggtcgagat
     7381 caggaagagg ctgtcggagg acgcgacgta ggtcgtgact cccgcgtgcg cggtcgcgcg
     7441 caccttggcc tgcagcgtcg agtaggaggc ctcgacgaag taggagagcg accgcggcgg
     7501 gccggggatg accgtgtaga gggtgccgag ccgcacggga taggtcgcgc cgccgtcggt
     7561 ggagaggtcg aactccacgg agtcggcggt cgcgcctccc acgaccaccg tcacggagtc
     7621 gggcttgccg ggcgggatct gcgcgccctt ggccgggtgc gtcaccgtcg cgaggaagag
     7681 tggcgcggcc gcggcctgaa tgtccagctt gccgtagccc cacacgttgt tcggcacggt
     7741 accggtgaag gcgtcggaac gcgccgtcga gcgaaggcga gccttgatcg ccgacggcga
     7801 cttcccggcc catgccgcct gggccaggag gatcgcgacc gatccggtga cgtgcggcga
     7861 cgacatgctg gtgcccgcct cgatgtggtg cacgccgtcc tgcgcgatga gcgccgtggc
     7921 cgggttg
//
LOCUS       DASSLZ010000288         5245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_721431, whole genome shotgun sequence.
ACCESSION   DASSLZ010000288 DASSLZ010000000
VERSION     DASSLZ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721431"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="VFM00_10925"
     CDS             complement(<1..1294)
                     /locus_tag="VFM00_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498803.1"
                     /translation="MHLAERPSRTATVLYPARNPEGRRFNAEDLWRIQRVGTPAAAPN
                     GSAIIVPVTSFDLDKNRGRTRLWLIPAEGAPRPLTSEEANAGDAVYSPDGTRLLFPRS
                     VGEERPQVHVMPLDGGEPERLTDMPLGVFDPRWLPGGTRIVFGAMLLEEAPTPEGTKE
                     LLEQRAKDPVKAHVTESRLYRYWDRWLTGGEVPHLFLLDLATRALTDLTPAGTGWFDF
                     MEPSGQYDIAPDGSEIAFVANSSRPPHHLLRWALFTVPVGGGAIRCLTPEGVPQGCAA
                     DMHRPRYAPDRRSIVYGAQKDPYFYADRVRLMRYDREAGTHTPMAEGWDRSPSAWEFT
                     PDGTLIIECEDEGRVSLFSMAPGGGTPRVIVQGGSAAALATGPGGRVTFTLQTITEPA
                     EACSCLPDGSDFRRHTAFNAPLLSEIAWGEVRTMEFEGA"
     gene            complement(1558..3402)
                     /locus_tag="VFM00_10930"
     CDS             complement(1558..3402)
                     /locus_tag="VFM00_10930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7498804.1"
                     /translation="MSETNPREGDPLQLAAAVADGAEIDWKRMESTGADAASVRSFEL
                     IDSVFRDYGKAAEEIRASLHAPLLATDSKWGSLRVLEQIGAGSFGQVFRAFDRSLQRD
                     VALKVLRDPPRSAAGVPEFLEEARRLARVRHPGVLAVYGVGVREGRAGIWTELVRGRT
                     LEELLESDGPFAPEETARIGIELCSALSAIHATGLIHTDVKTENVLREDGGRVVLADF
                     GAMDDRFDEDGSGSGYGSLLSVAPEILRGARPTPASDVYSLGVLLYRLLTGRYPLLAD
                     SPRDLLLAIGRGRTPLAEARPGIPGALAAAVDRALEPDPRRRYAGPNAMAQALKLALD
                     PTLPAKRVPAETYQPAGRDSMAAALGACAVISALVATGISQWGIPWLAHRYQGDATGR
                     AYATSTVVAARVENAPSGVAPSGVVESPAAPASQAPAGLRAMPVLYRISEVGKEPLAD
                     GATIRSGDALSLAVETGPDPVTCYVLDEDGAGKDYVLFPLSGYRARNPIAPATSQLLP
                     DGEGAKARAWVIDSENGRETVALITSLSKLPWLESMLRDVPRAGGDDASPQTASAGEV
                     VTRGIGGTMPVPVTGKMAAILDRLRASEAFRSGRAGLWRIRLKAPPSP"
     gene            complement(3399..3989)
                     /locus_tag="VFM00_10935"
     CDS             complement(3399..3989)
                     /locus_tag="VFM00_10935"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7498805.1"
                     /translation="MLPSSQDGDGQTLNPTRTLLERARAGDQRAFAQLFQRYHMALMR
                     WAHGRLCASARGQNETRDLVHICLSKAFHSLDGFVYRHQGSFLGYLHEILKNEVASAN
                     RRWMTKKPSAELDGDYPDSALRPDEEYEAKERKERYERALASLTPREQELIVMREMGL
                     THAEIAAELDLPTANAARMAVTRAIERLAAAIRENA"
     gene            complement(4129..>5245)
                     /locus_tag="VFM00_10940"
     CDS             complement(4129..>5245)
                     /locus_tag="VFM00_10940"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498806.1"
                     /translation="QQLLSVQGGPCVSIHLATHRRYPDWTQDPIQFKTLLAKAESELA
                     ARPAKDQATLLDPMRRLLDDPGVWEYALDGLAVYAAPTYAAVFRVPMPLPEAVVVADT
                     FHTKPLFRFLRSNSRYYVLAISQNAVTLYHGSSFGAEPVELAALPSDLTAALGASELD
                     EHQRGVVVHGGGFGGRAYHGRGPGKEERKETLLKFFRAVDKGLREYLSHERAPLILAA
                     VKYYRPIYKEANTYPHLLEEGPEGNFDRANGETIHAAAWPIVTRERERRVAEWVDQYR
                     SLASKGLALDGVEPVAAAVVQGRVRSVLAAENGNTWGRLDRKTGEVTLFQGPNGHANP
                     DLLDDVCEEAWKRGAEIYVLSQPELPTERPIAAVLRF"
BASE COUNT          787 a         1818 c         1869 g          771 t
ORIGIN      
        1 cggcgccctc gaattccatc gtccgcactt cgccccaggc gatctccgac aggagcggcg
       61 cgttgaacgc ggtgtggcgg cggaagtcgc tgccgtcggg caggcaggag cacgcctcgg
      121 ccggctcggt gatcgtctgg agcgtgaacg tgacgcggcc gcccggtccc gtggcgagcg
      181 ccgcggccga gcccccctga acgatgaccc gcggcgtgcc acctccgggc gccatcgaaa
      241 agagcgatac gcgcccctcg tcctcgcatt cgatgatgag cgtcccgtcc ggcgtgaact
      301 cccaggccga cggagagcgg tcccaccctt cggccatcgg cgtgtgcgtc cccgcctcac
      361 ggtcgtagcg catgagacgc acgcggtcgg cgtagaagta gggatccttc tgcgcgccgt
      421 agacgatgga gcgcctatcc ggagcgtagc ggggccggtg catgtcggcc gcgcaaccct
      481 ggggcacccc ctcgggcgtg aggcagcgga tcgcgccgcc gcccacgggc accgtgaaga
      541 gcgcccagcg gagcagatgg tgcggcggcc ggctggagtt ggcgacgaag gcgatctccg
      601 agccgtccgg ggcgatgtcg tactgtccgg agggctccat gaagtcgaac cagcccgtcc
      661 ccgcgggcgt caggtcggtg agcgcgcgcg tggccagatc gagcaggaac aggtgcggga
      721 cttccccgcc ggtcagccag cggtcccagt agcggtacag ccggctctcg gtcacgtgcg
      781 ccttgaccgg atccttcgcg cgctgttcca agagctcctt cgtcccctcg ggcgtcggcg
      841 cctcctcgag gagcatcgct ccgaagacga tccgcgtccc gcccgggagc cagcgcgggt
      901 cgaagacccc gagcggcatg tcggtcagcc gctccggctc gccgccgtcc agcggcatga
      961 cgtggacttg cgggcgctct tccccgaccg aacggggaaa caggagccgg gttccgtcgg
     1021 gcgaatagac ggcgtcgccc gcgttcgcct cctccgacgt gagcgggcgg ggcgcgccct
     1081 cggccggaat cagccagagc cgcgtgcggc cgcggttctt gtcgagatcg aacgacgtga
     1141 ccgggacgat gatcgcgctg ccattcggag ccgcggcagg cgtgccgacc cgctggatgc
     1201 gccagaggtc ctcggcgttg aagcgccgtc cctcgggatt tctcgcgggg tagagaacgg
     1261 ttgctgtgcg ggacggacgc tcggcgagat gcaaggcgct cctccgaaga acgagcaggt
     1321 gagcccgaaa cggctgggga gatacgaact tccattctac gtccgccgtt cgccgggggg
     1381 aagggtcggc tgcaacgcaa agaaaggcgg ggccggccgc tccccacggc cgaaccccgc
     1441 ccccggcgac gcccgccagg cgggcgtcga ttcgatcggc ttcgacatct gcgttgctcg
     1501 ttcaggtaaa cgaccggcgg gctgaaagcg cacgcgaaaa tcgcgcggtt tttgcggcta
     1561 agggctcggc ggcgccttca gacggattcg ccagagaccc gcccggcccg accggaaggc
     1621 ctcgctggcg cgaagccggt ccaggatggc ggccatcttc ccggtgaccg ggaccggcat
     1681 cgtgccaccg atgccgcgcg tgacgacttc gccagccgaa gccgtttgag gcgaggcatc
     1741 gtcgccgccg gcgcggggta cgtcgcgaag catggactcg agccagggga gcttcgagag
     1801 cgacgtgatc agcgccaccg tctcacggcc gttctccgag tcgatcaccc aggcgcgagc
     1861 cttggcgcct tccccgtccg gcagcaactg gctcgtcgcc ggggcgatcg ggttccgcgc
     1921 ccgatagccg gagagcggga agagcacgta atccttcccc gccccgtcct cgtcgaggac
     1981 gtagcaggtc acgggatcgg ggccggtctc gacggcgagg gagagcgcgt cgcccgaacg
     2041 gatcgtggcg ccgtcggcga gcggttcctt tccgacctcg gagatgcggt agaggacggg
     2101 catggcgcgg agtcctgccg gtgcctgact tgccggcgcg gccggggact cgaccacacc
     2161 ggacggcgcg accccggacg gggcgttctc gacgcgcgcg gccacgacgg tgctcgtggc
     2221 gtacgctctc cctgtcgcgt cgccctggta acgatgcgcc agccagggga tgccccactg
     2281 cgagataccc gtcgcgacga gcgcggagat cacggcgcag gccccgagcg ccgcggccat
     2341 ggaatcgcgc ccggcaggtt ggtaggtctc cgccggcacg cgcttcgccg gcagcgtggg
     2401 atcgagcgcg agcttgagcg cctgggccat cgcgttcggc cccgcgtagc ggcgcctggg
     2461 atcgggctcg agggcacggt ccaccgcggc ggccagggcg cccgggattc cggggcgcgc
     2521 ttccgccagc ggcgtccgcc cgcgcccgat cgcgagcagg aggtcgcgcg gcgaatcggc
     2581 gaggagagga tagcggccgg tgaggaggcg gtagaggagc acgccgagcg agtagacgtc
     2641 ggaggcgggc gtcggccgtg ccccgcgcag gatctcgggc gcgaccgaaa ggagcgagcc
     2701 gtaccccgag ccggagccgt cctcgtcgaa ccgatcgtcc atcgcgccga agtcggccag
     2761 gaccacccgg cccccatcct cgcgcagcac gttctccgtc ttcacgtccg tatggatcag
     2821 cccggtcgcg tggatcgcgg agagcgcgct gcacagctcg atcccgatcc gcgcggtctc
     2881 ttcgggagcg aaggggccgt ccgactcgag gagctcctcc agcgtgcggc cgcggaccag
     2941 ctcggtccag atccccgcgc gcccttcgcg cacgcccacg ccgtacacgg cgagcacgcc
     3001 cgggtggcgc acgcgggcca gacgccgcgc ctcttcgaga aactcgggca cgccggccgc
     3061 ggaacgcggc ggatcgcgca gcaccttgag cgccacgtcg cgctgcagcg agcggtcgaa
     3121 agctcgaaac acctggccga aggagccggc cccgatctgc tccaggaccc ggagcgatcc
     3181 ccacttggag tcggtcgcca ggagcggcgc gtgcaggctg gcccgtatct cctcggctgc
     3241 ctttccgtag tcgcggaaca ccgagtcgat cagctcgaac gatcggacgc tggccgcgtc
     3301 ggcgcccgtc gattccatcc gcttccagtc gatctcggcc ccgtcggcga cggcggccgc
     3361 cagctgcaac ggatcgccct cgcgggggtt cgtttcgctc atgcgttctc ccggatcgcc
     3421 gccgcgagcc gttcgatcgc ccgcgtcacg gccatccgcg cggcgttggc cgtgggaagg
     3481 tccagctcgg cggcgatttc agcgtgggtg agacccatct cgcgcatcac gatcagctcc
     3541 tgctcacggg gcgtgagcga cgccagcgcc cgctcgtacc gctccttgcg ttccttggct
     3601 tcgtactcct cgtcgggccg gagcgcggaa tcggggtaat ccccatccag ctccgcgctt
     3661 ggcttcttgg tcatccaccg gcggttcgcc gaggcgacct cgttcttgag gatctcgtgc
     3721 agatatccca ggaacgagcc ctggtgcctg tagacgaacc catccaggga gtgaaacgcc
     3781 ttggagaggc aaatgtgcac aaggtcccgc gtctcgttct ggccgcgggc ggaggcgcaa
     3841 agccttccgt gggcccagcg catgagggcc atgtggtacc gctggaagag ctgggcgaag
     3901 gcgcgttgat cgccggcccg cgcgcgctcg aggagcgtgc gcgtgggatt cagggtctgg
     3961 ccgtcgccgt cctggctcga ggggagcacg atgacgactt ttcctcagcg aggagatccg
     4021 agcgtagggg gcttacaagg gactgattat acataactta cagtggactt caagccggaa
     4081 aaagccctgc cgcgctggag ccgggcggcg cggcagggcc gggatgacct agaagcgaag
     4141 gaccgcggcg atcggccgct cggtcggaag ctccggctgg gagaggacgt agatctccgc
     4201 gccgcgcttc cacgcctcct cgcagacgtc gtccagaagg tcgggattgg catggccgtt
     4261 cggcccctgg aacagcgtga cctcgcccgt tttccggtcg agacgccccc aggtgttccc
     4321 attctccgcg gcgaggaccg agcgcacgcg tccctggacc accgccgccg ccaccggctc
     4381 gacgccgtca agggcgaggc ccttcgaggc gagggatcgg tactggtcca cccactcggc
     4441 cacgcggcgc tcccgctcgc gggtcacgat cggccatgcg gcggcgtgga tggtctcccc
     4501 gttcgcgcgg tcgaagttcc cctccggacc ctcctcgagc aggtgcggat aggtgttcgc
     4561 ctccttgtag atcggccggt agtacttcac cgccgcgagg atcagcggcg cccgctcgtg
     4621 gctcaggtac tcgcggaggc ccttgtccac ggcacggaag aacttgagca gggtctcctt
     4681 tcgctcttcc tttccgggac cgcggccgtg atacgcgcgc cccccgaagc cacccccgtg
     4741 cacgacgacg ccccgctgat gctcgtccaa ctcgctcgcc cccagcgcgg ccgtcaggtc
     4801 ggagggcagc gccgcgagct ccaccggctc ggcgccaaag gagctgccgt gatagagggt
     4861 gaccgcgttc tggctgatgg cgagcacgta gtagcgcgag ttcgatcgca gaaaccggaa
     4921 gagcggcttg gtgtggaagg tgtccgcgac gacgaccgcc tcggggagcg gcatcgggac
     4981 gcggaagaca gcggcatagg tgggcgccgc gtagacggcc aggccgtcga gcgcgtattc
     5041 ccagactccc ggatcgtcga gaaggcgccg catgggatcg agcagcgtcg cttgatcctt
     5101 cgcgggcctc gccgcgagct ccgattccgc cttggccagc agggtcttga actggatcgg
     5161 gtcctgcgtc cagtccggat agcggcggtg cgtcgcgaga tggatcgaga cgcagggccc
     5221 gccctgaacc gacaggagct gctgc
//
LOCUS       DASSLZ010000289         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_487868, whole genome shotgun sequence.
ACCESSION   DASSLZ010000289 DASSLZ010000000
VERSION     DASSLZ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487868"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1234
                     /gene="bshA"
                     /locus_tag="VFM00_10945"
     CDS             65..1234
                     /gene="bshA"
                     /locus_tag="VFM00_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HET7498807.1"
                     /translation="MKIGITCYPTHGGSGVVATELGMELAKRGHEVHFISYQVPFRLK
                     PYQDNVYFHEVQITTYPLFQYPPYTLALAAKMAEVADEAELDVLHAHYAIPHAICGYL
                     ARQIAKSTKLRMVTTLHGTDITLVGSDTSFRGLTTFGIEQSDGVTAVSEFLKRKTIEV
                     FSPRRAIRVIPNFVDTERYAPRPPSECRRERYAKKGERILVHISNFRPSKRVSDAVRV
                     FAAVRREVPAVLLMVGDGVERSQSREVAVELGVERYVRYLGQMDAVEDVLGCGDLFLL
                     PSEHESFGLAALEAMSSGVPVIGTTAEGLPELIRPGETGYLLAVGDVEGMSRRAIEIL
                     SDTEKREAMAAASRRIAVEKYDASSIIPLYENFYAETLDLPPRMPAHLAPPEGLA"
     gene            complement(1143..2393)
                     /locus_tag="VFM00_10950"
     CDS             complement(1143..2393)
                     /locus_tag="VFM00_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET7498808.1"
                     /translation="MLRAAAFAALLLLALGLTSCGDTPTAPKAIVPVSGTILDRDGNP
                     IQAVYIYFEDRGPHTAGSDPFIGRAITNVSGQYSASMPEGRYRIYILPPSFTAYAGIV
                     LDDRTIQRPGLRLDHRFQWARLAIHPTASGGQTAADLRAYVFPEADDAYSHYFFQGDT
                     RLSGSEREAFVPAGSYRVQLYLYGIGFGYPVQTRSVSFSADTTLEAAFTGNVVTVHVT
                     GQNGIPMENAHVTAYSPSATVPAITDAAGSATLYMPSATYRMQVASPSSEIMPRSMVS
                     LVVSADATIDFDLSGTEWSGILRRISDDQPIPDVGVEADDINGTRGAFSQTDGSGRFH
                     FIVATGRLYRITAYDDQWRTLGSFEPVASGSDSTFDLRVDPSTAHYSGQTPRFRPTPP
                     VAPGAPASAAVDPAFRRRSFRTGG"
     gene            complement(2393..>2817)
                     /locus_tag="VFM00_10955"
     CDS             complement(2393..>2817)
                     /locus_tag="VFM00_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498809.1"
                     /translation="EAAVGMRFGLGLDRHVSLLMDVTYTTPTRETTGSLARVTSVRSL
                     VQYRYLAGPVHPYLIAGLGGILFDFDDTYDAAGGTLTGGCGIEVRIARQVALFGEASI
                     DWYRSRTTSYAPTGEVLDRGARHTDEARTFMAGLQARF"
BASE COUNT          486 a          914 c          973 g          444 t
ORIGIN      
        1 gcctcagcga gtcgctgggc gcgccgggaa gccacacgct gattccgctg ggggtatcgg
       61 acgaatgaag atcgggatca cctgctatcc gacgcacggc ggctcgggcg tggtcgcgac
      121 cgagctcggg atggagctcg ccaagcgcgg gcacgaggtg cacttcatct cctaccaggt
      181 cccgttccgc ctgaagccct accaggacaa cgtctacttc cacgaagtgc agatcacgac
      241 ctacccgctc ttccagtatc cgccctacac gctggcgctc gcggcgaaga tggcggaggt
      301 ggccgacgaa gcggagctgg acgtgctgca cgcgcactac gccatcccgc acgccatctg
      361 cggctacctc gcgcgccaga tcgcgaaatc caccaagctc cgcatggtga ccacgctcca
      421 cggcaccgac atcacgctgg tgggctcgga cacctcgttc cgcggcctga ccacgttcgg
      481 catcgagcag agcgacggcg tgaccgcggt ctcggagttc ctgaagcgga agacgatcga
      541 ggtcttctcg ccccgccgcg cgatccgcgt gatcccgaac ttcgtggaca ccgagcgcta
      601 cgccccaagg ccccccagcg agtgccgccg cgagcggtac gcgaagaagg gggagcggat
      661 cctggtccac atctcgaact tccgcccgag caagcgcgtc tccgacgccg tgcgcgtctt
      721 cgccgcggtg cgccgggaag tgccggccgt gctgctcatg gttggcgacg gcgtggagcg
      781 ctcccagagc cgcgaggtcg cggtcgagct gggcgtggaa cgctacgtgc ggtatctggg
      841 tcagatggac gcggtcgagg acgtgctggg ctgcggggat ctcttcctgc tcccgagcga
      901 gcacgagagc tttggactgg cggcgctgga ggcgatgtcg agcggcgtgc cggtgatcgg
      961 caccaccgcc gaggggcttc ccgagctgat ccgccccggc gagaccggct acctgctggc
     1021 ggtcggcgac gtggagggga tgtcgcgccg tgcgatcgag atcctgagcg ataccgagaa
     1081 gcgcgaggcg atggccgcgg cgtcgcggcg catcgccgtc gagaagtacg acgcgagcag
     1141 catcatcccc ctgtacgaaa acttctacgc cgaaacgctg gatctaccgc cgcggatgcc
     1201 ggcgcacctg gcgccaccgg aggggttggc ctgaagcggg gcgtttgacc gctgtagtgg
     1261 gccgtcgacg ggtccacccg aaggtcgaac gtggagtcgg agccggacgc caccggttcg
     1321 aaactgccca gcgttcgcca ttgatcatcg taggcggtga tgcggtacag gcgtcccgtc
     1381 gcgacgatga agtgaaaccg gccgctccca tccgtctgcg agaacgcacc ccgggtcccg
     1441 ttgatgtcat cggcttcgac ccccacgtcc gggatcggct ggtcgtcgct gatcctcctg
     1501 agaatgccgc tccactccgt tccggagaga tcgaagtcga tcgtcgcgtc ggcgctcacg
     1561 accaagctga ccatgcttcg cggcatgatt tcggagctcg gggaggcgac ctgcatccgg
     1621 taggtggcgg agggcatgta cagcgtcgcg ctcccagccg cgtccgtgat cgcgggaacg
     1681 gtggctgatg gggagtacgc ggtgacgtga gcgttttcca tgggaatccc gttctgcccc
     1741 gtcacatgga ccgtcacgac gtttcccgtg aacgcagcct cgagcgtggt gtcggccgaa
     1801 aacgataccg agcgtgtctg gaccggatac ccgaatccga tgccgtacag gtaaagctga
     1861 acgcggtagc tgcccgcggg aacgaacgcc tcgcgctcgc tacccgaaag acgcgtatct
     1921 ccctggaaaa agtagtggga gtaggcgtcg tcggcctccg gaaagacata cgcccggagg
     1981 tcggccgcgg tctgtccgcc ggacgcggtg gggtggatgg cgagacgggc ccattggaac
     2041 cggtggtcca ggcgaaggcc gggccgctgg atcgtacgat cgtcgagcac gatcccggca
     2101 tacgccgtga aggacggagg aaggatgtag atccggtaac gtccttcggg catagaggcc
     2161 gagtactgcc cgctcacgtt cgtgatcgcg cgcccgatga aagggtcgct gccggcggtg
     2221 tgcggcccac ggtcctcgaa gtagatgtag accgcctgaa tcgggttgcc gtctcgatcc
     2281 aggatcgtgc ccgaaaccgg gacgatcgcc ttcggcgctg tcggcgtgtc gccgcaggag
     2341 gtcaggccca gtgccaggag caggagagcg gcgaacgccg cggcccggag catcagaacc
     2401 tcgcctgaag cccggccatg aaggttcgag cttcgtcggt atgccgagct ccccgatcca
     2461 gcacctcgcc cgtcggcgca tagctggtcg tacggctgcg ataccagtcg atcgaggcct
     2521 ccccgaagag agcgacctgg cgcgcgatcc tgacctcgat cccgcaaccc ccggtcaggg
     2581 tcccgcccgc cgcgtcgtag gtgtcgtcaa agtcgaagag gatgccgccg agtcccgcga
     2641 tgagataggg gtgaaccggg ccggccaggt agcggtactg caccagcgaa cggaccgaag
     2701 tgacgcgggc gaggctcccg gtggtctcgc gcgtgggtgt cgtgtaggtc acgtccatga
     2761 gcaggctcac gtgccggtcc aggccaaggc caaatcgcat tccgaccgcg gcctcgg
//
LOCUS       DASSLZ010000290         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_615867, whole genome shotgun sequence.
ACCESSION   DASSLZ010000290 DASSLZ010000000
VERSION     DASSLZ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615867"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VFM00_10960"
     CDS             complement(<1..969)
                     /locus_tag="VFM00_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET7498810.1"
                     /translation="MPRIYLDHNASTPVRAEVLDAMLPYFAEHYGNASSAHAFGQEAK
                     GAIEEARAKVAALVNAAPAEIVFTSGGTESDNMGVLGAARARAATGRHVVVSEIEHEA
                     VRNAAGALEAEGFAVTRVPAMADGRVDPDAVAKALKPDTVLVSVMAANNETGVLQPVE
                     AIGALCAGRGIAFHTDAVQAAGKIPIDARAWQATYLTIAAHKFYGPKGVGALYVKRGA
                     KTAPLLFGGEHERGRRPGTENVPGIVGMGAAAELARAELDTAPPRMAALRDRLEQGIL
                     ARVPQVVVHGAAAPRACNTSHLSFVGAEGEHLLLTLDQKGIACSSGS"
     gene            complement(1104..1982)
                     /locus_tag="VFM00_10965"
     CDS             complement(1104..1982)
                     /locus_tag="VFM00_10965"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7498811.1"
                     /translation="MFGTTRLWIGAAAAIGILAAGSAFAAGAAKGKAAAKSVGSPDDV
                     AVMETTKGRMVIEFWPKEAPKTVANFKKLARSGFYNGTGFHRIIAGFMVQGGDPKSKN
                     PKAPDLGTGGPGWTIPDEFNDHKHEKGVLSMAHTAEPNSGGSQFFLMHGKAPFLDHKY
                     TAFGHVIEGMDVVDAIANVRTAPNPMMPGENSKPLEWVTVKSVKIVPRAVAMGGGAKA
                     AVATEKAASAKAAGKAADASNDAAEKAAEKATDAKESADKAAEAAKKAGTKAAEAAQS
                     ANEAAKAAGTDSSAAK"
     gene            complement(2098..>3257)
                     /locus_tag="VFM00_10970"
     CDS             complement(2098..>3257)
                     /locus_tag="VFM00_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7498812.1"
                     /translation="RPADLSGASARAGAPRSDARAHVSAGRDYLSRGLTDEAEREFRL
                     ALESEPGNQEALAGLGQVRVKRGAYAEGAEMLERATRSSTQMAAAFRSLGDAYAGMGQ
                     LDKAADAYRQAVALTPGDLDSRLALAHSLTEIGEYDEAAGLCTAAARGVKNQPAALAQ
                     VYRQLGEVRSRQGSVPEAMSAYYRAAELAPDDPEVVRGTADAAVRGGLYAEAATALDH
                     VLRIAPLDVAAKKQLGWVNFKLERYPVAIKDYEAVGDSLGTADRYYLAQAYAKSSKTD
                     RAVELFREVARQDPQNYKGVYCNMAYAYFDANRYQRAIDTAREGLKSDSGNACLRFCW
                     AQALDKLGRHDEAIPVFEAAVADPAYAEPARRELERQRRIVKLLKTKDRGN"
BASE COUNT          399 a         1217 c         1107 g          534 t
ORIGIN      
        1 ggagccgctg gagcaggcga tccccttctg gtcgagcgtg aggagcaggt gctccccctc
       61 ggcgcccacg aaggagaggt ggctggtgtt gcaggcgcgg ggcgcggcgg cgccgtgcac
      121 cacgacctgc ggcacgcgcg ccaggatccc ctgctccagc cggtcgcgca gcgccgccat
      181 gcgcggcggc gcggtatcca gctccgcccg ggccagctcg gcagccgcgc ccatgccgac
      241 gatcccgggg acgttctccg ttccgggccg gcggccgcgc tcatgctcgc cgccgaagag
      301 aagcggggcc gtcttcgcgc cgcgcttcac gtagagcgcg cccactccct tcggtccgta
      361 gaacttgtgc gccgcgatcg tgagataggt cgcctgccag gcgcgcgcgt cgatcgggat
      421 cttccctgcg gcctgcacgg cgtcggtgtg gaacgcgatc ccccgcccgg cgcagagcgc
      481 gccgatcgcc tcgaccggct gcagcacccc ggtctcgttg ttggcggcca tcaccgagac
      541 gaggacggtg tcgggcttga gcgccttcgc cacggcgtcg ggatcgacgc gcccgtccgc
      601 catcgccggg acgcgcgtca cggcgaaccc ctcggcctcg agcgcgcctg cggcgttgcg
      661 caccgcctcg tgctcgatct cgctcaccac cacgtgccgc cccgtcgcgg cgcgcgcgcg
      721 cgcggccccg aggacgccca tgttgtccga ctcggtgccg ccgctcgtga acacgatctc
      781 ggccggcgcg gcgttcacca gggccgccac cttcgcccga gcctcctcga tcgcgccctt
      841 cgcctcctgg ccgaaggcgt gcgcgctcga ggcgttgccg tagtgctccg cgaagtacgg
      901 gagcatcgcg tcgagaacct cggcgcggac gggcgtgctg gcattgtggt cgagatagat
      961 ccggggcaac ggaagcctcc gatggggacg tgcgctgcgg gaagcgcggc tccttcgagg
     1021 gtaacaaagc gcgcgggccg gacgcaaatc ggacgcccag cccgcgcggg gaggagcccg
     1081 gaggccccta ggattcgctg acgttacttg gccgcgctcg aatcggtgcc cgcggccttg
     1141 gcggcctcgt tcgcggactg cgcggcttcc gcggccttgg tgcccgcctt cttggcggct
     1201 tcggcagcct tgtccgccga ttccttggcg tccgtcgcct tctccgccgc cttctcggcc
     1261 gcgtcgttgc tcgcgtcggc ggccttcccg gccgccttcg ccgacgcggc cttctcggta
     1321 gcgaccgccg ccttagcgcc gccgcccatg gcgaccgcgc gcgggacgat cttcaccgac
     1381 ttcacggtga cccactcgag cggcttggag ttctcgccgg gcatcatcgg attgggagcc
     1441 gtgcgcacgt tcgcgatcgc gtccacgacg tccatcccct cgatcacgtg gccgaaggcc
     1501 gtgtacttgt gatccaggaa cggcgccttg ccgtgcatca ggaagaactg cgatccgccg
     1561 gagttcggct cggcggtgtg cgccatcgag agaacgccct tctcgtgctt gtggtcgttg
     1621 aactcgtcgg ggatcgtcca gcccgggccg ccggtgccga gatccggagc cttcggattc
     1681 ttggacttgg gatcgccgcc ctggaccatg aagccggcga tgatgcggtg gaagccggtg
     1741 ccgttataga agccgctgcg ggcgagcttc ttgaaattcg cgacggtctt gggagcctcc
     1801 ttgggccaga actcgatcac catgcgcccc ttggtcgtct ccatgaccgc gacgtcgtcg
     1861 gggctgccca cggacttggc cgcggccttt cccttggccg cgccggccgc gaacgccgac
     1921 cccgccgcca gaatcccgat ggccgccgcc gcgccaatcc acagtctcgt cgtgccaaac
     1981 atcgaacctc cttgccgttg cgctgtcccg atggccattc ccggccaccc cttgtcgtcc
     2041 ggatccactc tcccgatacg cgttacttaa tgtgcgtctc ccacgctccg gttcccctca
     2101 attgccccgg tccttcgtct tcagaagctt gacgatccgc cgctggcgct ccagctcgcg
     2161 gcgggccggt tcggcgtagg cggggtcggc cacggccgcc tcgaacaccg gaatggcctc
     2221 gtcgtgacgc cccagcttgt ccagggcctg ggcccagcag aagcgcaggc aggcgttgcc
     2281 gctgtcgctc ttcagcccct cgcgggccgt gtcgatcgcg cgctggtacc ggtttgcgtc
     2341 gaagtaggcg tacgccatgt tgcagtagac ccccttgtag ttctgagggt cctgccgcgc
     2401 gacttctcgg aacagctcca ccgcgcggtc ggtcttggac gacttggcgt acgcctgcgc
     2461 caggtagtag cggtcggccg tgcccagcga atcgcccacc gcctcgtagt ccttgatggc
     2521 gaccggatag cgctccagct tgaagttcac ccagccgagc tgcttcttcg ccgccacgtc
     2581 gagcggcgcg atccggagaa cgtggtcgag ggcggtcgcc gcctcggcgt agagcccgcc
     2641 gcgcaccgcg gcgtccgccg tcccgcgcac gacttccgga tcgtccggag ccagctcggc
     2701 cgcgcggtag taggccgaca tcgcctcggg gacgcttccc tgccgcgagc ggacctcgcc
     2761 caactggcgg tagacctgcg cgagcgcggc gggctggttc ttcacgccgc gcgccgcggc
     2821 ggtgcagagc cccgccgcct cgtcgtattc gccgatctcg gtgagcgaat gcgccagcgc
     2881 gagccgcgag tcgagatcgc ccggcgtcag cgccaccgcc tgccggtacg cgtcggccgc
     2941 cttgtccagc tgccccatcc cggcgtacgc gtcccccagc gagcggaagg ccgcggccat
     3001 ctgcgtcgac gagcgggtcg cgcgctcgag catttcggcg ccctcggcgt acgcgccccg
     3061 cttcacccga acctggccca gacccgcgag cgcctcctgg tttccgggct cggactcgag
     3121 agccagccgg aactcgcgct ccgcctcgtc ggtgaggccg cggctcaggt agtcgcgtcc
     3181 ggcgctgacg tgcgcgcgcg cgtccgagcg gggagcgccg gcgcgcgccg acgcaccgga
     3241 gaggtccgcc gggcggg
//
LOCUS       DASSLZ010000291         8704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_363529, whole genome shotgun sequence.
ACCESSION   DASSLZ010000291 DASSLZ010000000
VERSION     DASSLZ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363529"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1008)
                     /locus_tag="VFM00_10975"
     CDS             complement(232..1008)
                     /locus_tag="VFM00_10975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7498813.1"
                     /translation="MKRLIAIAALALLSIAPVTSRAGMITLTQGSTKVKGYLAIPAGE
                     GTHPAIVVIHEWWGLNDWVKEQADRLAKEGYVAFAVDIYHGQVAKDEATAHQLMSGLD
                     ETEALNTLRAATDFLRGRSDVRANAIGVIGWCMGGGYSIRLAAADPGIRACVMYYGAP
                     ISDPAAIRGLQAAVLGNFGGEDKGPSPDQVRTFEKALKQAGKRVDFKIYPGAPHAFAN
                     VHNPWGGYREAAAKDAWLRTKAFLRKELKMGSLPRRSRTG"
     gene            complement(1058..2086)
                     /gene="queG"
                     /locus_tag="VFM00_10980"
     CDS             complement(1058..2086)
                     /gene="queG"
                     /locus_tag="VFM00_10980"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018141997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET7498814.1"
                     /translation="MRERLREAALALGFSAVGFASAEPHEHRARAAAWIAEGRHGTMR
                     WMARQTERRGDPSALLPGAKTVISVAASYYRGGWPAPDGAPRGAVARYAWGRDYHKRM
                     KRRLVRLGRTLPALAPGAGWTTAVDARPFLDRAFAERAGVGWIGKNTNLIRKRAGSWC
                     FLGAIVTDIALPPDAPARNYCGTCVRCIGACPTGAITAPYRLDARRCISYLTIEHDGP
                     IPLEMRPLIGTRIFGCDDCQEVCPWNRFAVPSADPDFAERPDQQAPELIPLLGLDGPA
                     FRKRFEGTALLRAGRDRFVRNVAVALGNAGDPRAIPALERAASHDTNAHVREHAAWAL
                     ACLRGDTR"
     gene            2113..2784
                     /locus_tag="VFM00_10985"
     CDS             2113..2784
                     /locus_tag="VFM00_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET7498815.1"
                     /translation="MAERFELQPLFPLPNVVLFPKAILPLHIFEPRYRTMMADALQGG
                     QTICMAMLRPGWEADYHGSPAVYGVGCVGRVVQHQLLEDGRYNIALHGESKVAIEGFT
                     QEEPYRIARVRTVEEDHAWASSSGVEGEVAELLALFRRVRLGESAAVDLTEMYGPHVS
                     PEAVLNMIAMNLKVEANVKQELLELDSLRSRFRSIVHYLRDSAAKQDLLDKVRHLYPG
                     DARRN"
     gene            2787..3665
                     /gene="nth"
                     /locus_tag="VFM00_10990"
     CDS             2787..3665
                     /gene="nth"
                     /locus_tag="VFM00_10990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET7498816.1"
                     /translation="MVPRGTSPPADVNLLIRRLEEAHPDAACALTHTNPFQLLVATIL
                     SAQCTDARVNQVTPVLFARFPDAEAMSLASQEELEALIRTTGFFRNKSKSIRGSSQRL
                     VEAFGGIVPRSMDELLSLPGVARKTANVVLGVGHGIAEGVVVDTHVYRVSRRLGLTRG
                     KGPVEVEQDLMQTIPRDKWIEFAHLLIFHGRRVCVARKPRCEACAVLDLCPSASYFLA
                     GKVPPWDRGGEAGEPRAAKKGPRSRVAAKVAAGGKATARKRAVPKPAASRKARKSSAP
                     RKLRKSAAARARRRAS"
     gene            3662..4165
                     /locus_tag="VFM00_10995"
     CDS             3662..4165
                     /locus_tag="VFM00_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET7498817.1"
                     /translation="MNPGAGLPGTVAPDFDVRLLEGGYQELRDLVQPGGGIVIFFKTE
                     CETSALLLERIGPLARALEGEERVFLAVAQNPAAEVRAFREQHRIAFPLACEEPPYPA
                     SRAYAVVTVPTLIVIDGTGRIAERVEGFVKSEVLALGPAVEQALALGDIPPVLDRPDE
                     LPELKPG"
     gene            4209..4946
                     /locus_tag="VFM00_11000"
     CDS             4209..4946
                     /locus_tag="VFM00_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET7498818.1"
                     /translation="MNETNGIAITWLGHATVLYRSPRGKQVLVDPWIEGNPASPPEAK
                     KLSGVDVMLITHGHGDHFADAIPLAKKFHPEIVCNYEISLFLESKGVKEKVHGINKGG
                     SVTVAGIRVTMVHAIHSSSIEDGDRIVPAGEPCGFVIEFEDGTRVYHAGDTGFFGDMK
                     WIGDLYKPSIAALPIGDLFTMAPREAAIAARLIGAAHVLPIHHSTFPALTGTPAELKR
                     QLADQSEIHVIEVKPGETVEPELARRS"
     gene            complement(5028..6644)
                     /locus_tag="VFM00_11005"
     CDS             complement(5028..6644)
                     /locus_tag="VFM00_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498819.1"
                     /translation="MKRGRARAARPARRGGGASAKARRASAPRRRASSDIRIFPNDPE
                     ASVGLVPATPIAPLDVDPSYVIDGRRYAPAPYDPGTLAFQYWQGETALRRTIRVWEDL
                     FDRDFAAWHDGHPLRVRLRAGRDLNAFYDRASLQFFYDTDRVTGRAVYACESLDVVAH
                     ETGHAVLDVYQPAFWSSADPEIAAFHEAFADCSSLLVTLTEPAVRRRFLDEAGAAIRK
                     SNLVSRLAEALGRAVYDNYGPRAVSDPTRLRDANNAFRYRPPTTLPAGASDDKLSSEP
                     HSFSRVFTGAFYDLLAWLIARALRETPEEPEPAVERARRLGGRLLARALETLPPGAGH
                     YRAVAQRMREVDQTEGGEVASAGIEEAFAKHGIKLPSPRPKTATVRGRIDTRAGTRGS
                     YRSLRALDPDRAGGAAAIRAALGLPRGARLERRTWSPRVGGGVREQLIHRDHVVVRDR
                     TLGSLSGIAVPVTCGCTLTREPDGRVEGASLRPHPHPEADEIAVWLRAWIARDAIRPK
                     DGTLSAVEHFRARQPFRVTRAGVLERVYFD"
     gene            complement(6641..8353)
                     /gene="polX"
                     /locus_tag="VFM00_11010"
     CDS             complement(6641..8353)
                     /gene="polX"
                     /locus_tag="VFM00_11010"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HET7498820.1"
                     /translation="MDKRDVARTLEQIAAILEIKGENPFKVRAYENAARAVDALSEDL
                     GTLIADKRLSEVRGIGSSIQKNVEELWTTGKMKYFEDLCETVPVGWFDMMRIPGLGPK
                     RIRTLCDLLQIASVEALKEAAEGGRIRGLKGFGEQSEKKILEGIAFLERGGRRELAAV
                     VRPIAEEILAALRDRKDVIRAELGGSLRRWLETVKDVDLLVATKKPDAVAKAFLKLLP
                     QASITAQGDTKVSVRLPTGLAVDLRLVTNEQFPFALHYFTGSVAHNVRVRQRAIERGF
                     SLNEYELSGGEHAPIASEADLFQVLGMAYVEPELREDRGEIEAAEVGRLPGLITARDL
                     KGILHCHTTYSDGKSTLEEMAEGAVAAGAEYLGISDHSQSVRYAGGLSEEDLLRQAKE
                     IDKWNSKSHDLRILKGAEVDIMPDGTLDYPDRVLERLEFVVASVHTNLNMGEDEMTAR
                     VVKALRNKHVSILAHPTGRLLLQREGYRIRFDEVFRVAAQEGVVVEINAHSRRLDLDW
                     REIPAGKALGVKFAIDPDAHHVSGYQDLRLGIGVARKGWLGPGDVVNTLPVEGALELF
                     RARR"
     gene            complement(8391..>8704)
                     /locus_tag="VFM00_11015"
     CDS             complement(8391..>8704)
                     /locus_tag="VFM00_11015"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET7498821.1"
                     /translation="KREIDVLRAIASSGASDLNPDLRSLGEKEDAYETTVERFRSVLA
                     EVSRHGCIVRDLELGLVDFYTMHREQIVCLCWRRGEPRIEHWHPLDEGFSGRRPLDEL
                     R"
BASE COUNT         1285 a         2980 c         3111 g         1328 t
ORIGIN      
        1 cctcctccag cgtgcaggag gcgtccagcc gcgcgccgtt gcagaagagg tcgatttcca
       61 gctcgcaggg attgtggcgg ggcagggaca tgggtagcgg tctcgcggtg atgaaacgcg
      121 ccggggtggc tgccccggcg cgtccgacta acccatgtaa tctagcaggc ttacgaggtc
      181 gattctatgg tacctaggta ccagcaggac acacttctgg ccgatgaggg atcagcccgt
      241 ccgggagcgc ctcggaaggg agcccatctt cagctccttg cggagaaagg ctttggtgcg
      301 aagccacgcg tcctttgccg cggcctcgcg gtagcctccc cagggattgt gcacgttggc
      361 gaaggcatgg ggcgcgccgg ggtagatctt gaagtccacg cgcttgcccg cctgcttcaa
      421 cgccttctcg aacgtccgga cctggtcggg ggacgggcct ttgtcctcac cgccgaagtt
      481 gccgaggacc gccgcctgga gcccgcggat cgcggcggga tcggagatcg gcgcgccgta
      541 gtacatgacg caggcgcgga tgccgggatc ggccgccgcg agccggatcg agtagcctcc
      601 acccatgcac cagccgatca cgccgatcgc gttcgcccgg acatccgagc gtccgcgcag
      661 gaagtcggtc gcggcgcgca gggtgttgag cgcttcggtt tcgtccaggc cgctcatgag
      721 ctgatgcgcg gtcgcttcgt ccttggcgac ctgcccgtgg tagatgtcca cggcgaaggc
      781 gacgtagccc tccttggcga ggcggtcggc ctgctccttc acccagtcgt tgagccccca
      841 ccactcgtgg atgacgacga tcgcgggatg cgtgccctcg ccggcgggga tggcgaggta
      901 gcctttcacc ttggtcgagc cctgcgtcaa ggtgatcatg cccgcgcggg acgtgaccgg
      961 agcgatggat agcagcgcca gtgcggcgat ggcgatcagt cgcttcatgg ggatgtgcct
     1021 ccgggttggc gttcggtcgt tcagcttcga cttggaatta tcgcgtatct ccccggaggc
     1081 acgccagcgc ccaggcggcg tgctcgcgca cgtgcgcgtt cgtatcgtgg gacgccgcgc
     1141 gttccagcgc ggggatggcg cgcgggtcgc cggcgtttcc gagcgcgacg gcgacgttcc
     1201 gcacgaagcg gtcccggccc gcgcggagaa gggccgtgcc ctcgaaacgc ttccggaacg
     1261 cggggccgtc caggcccagc agcgggatca actccggcgc ctgctggtcc gggcgctccg
     1321 cgaagtcggg atcggcgctg gggacggcga agcggttcca ggggcagacc tcctggcaat
     1381 cgtcgcagcc gaagatgcgg gtgccgatca gcggccgcat ctcgagcgga atcggtccgt
     1441 cgtgctcgat cgtgaggtag ctgatgcagc gccgggcgtc cagccggtag ggagcggtga
     1501 tcgcgccggt gggacaggcg ccgatgcagc gaacgcacgt gccgcagtag ttgcgcgccg
     1561 gcgcgtcggg aggaagcgcg atgtcggtca cgattgcccc gaggaagcac caggagcccg
     1621 cgcgcttccg gatcaggttc gtgttctttc cgatccatcc cacgcccgcc cgctcggcga
     1681 acgcgcgatc caggaagggg cgggcgtcca cggcggtcgt ccagcccgct ccgggggcga
     1741 gtgccgggag cgtgcggccc aggcggacca gcctgcgctt catccgcttg tggtagtcgc
     1801 gtccccaagc gtagcgggcc acggcgccgc gtggagcgcc gtccggggcc ggccagccgc
     1861 cgcggtagta gctggcggcc accgagatca cggtcttcgc gcccgggagc agcgccgagg
     1921 gatcgccccg gcgttcggtc tgccgcgcca tccagcgcat cgtgccgtgg cgtccctcgg
     1981 cgatccacgc ggcggcccgc gcccggtgct cgtgcggctc ggccgaagcg aatcccaccg
     2041 cggaaaaccc aagcgccaga gccgcttccc gaagtctctc ccgcagaaaa tcgcttgtgc
     2101 tagcatcggc cggtggccga gcggttcgaa cttcagcctc tgtttccact tccgaatgtc
     2161 gtcctcttcc cgaaggcgat cctgccgctc catatcttcg agccgcgcta ccgcaccatg
     2221 atggccgatg cgctccaggg tggccagacg atctgcatgg ccatgctgcg ccccggctgg
     2281 gaagccgact accacggctc gcccgcggtc tacggcgtcg ggtgcgtcgg acgcgtggtg
     2341 cagcatcagc ttctcgaaga cgggcgctac aacatcgcgc tgcatggcga gtccaaggtg
     2401 gccatcgaag ggttcaccca ggaggagccc taccggatcg cccgggtccg gacggtcgag
     2461 gaggatcacg cctgggcgtc ctccagcggc gtggagggcg aggtcgccga gctgctcgcc
     2521 ctgttccggc gcgtgcggct cggcgagagc gcggcggtgg acctgacgga gatgtacgga
     2581 ccccacgtga gccccgaggc ggtcctgaac atgatcgcga tgaacctgaa ggtggaggcg
     2641 aacgtgaagc aggagctttt ggagctggac tcgctgcgat cgcgcttccg ctccatcgtg
     2701 cactacctgc gcgactcggc ggcgaagcag gacctgctcg acaaggtccg ccacctctat
     2761 cccggcgacg cccggcggaa ctgaccatgg tccctcgagg aacgtcgccg ccggcggacg
     2821 tgaatctcct gatccgccgt ctcgaagagg cgcaccccga cgccgcgtgc gcgctcacgc
     2881 acacgaaccc gttccagctc ctcgtcgcca cgatcctctc cgcgcagtgc accgacgcgc
     2941 gcgtgaacca ggtgaccccg gtgctgttcg cgcgcttccc ggatgccgaa gcgatgagcc
     3001 tggcctcgca ggaggagctg gaggcgctga tccggaccac cggtttcttc cggaacaagt
     3061 cgaagtcgat ccgcggctcg tcgcagcgat tggtggaggc gttcggcggc atcgtgccgc
     3121 gcagcatgga cgagctcctc tccctgcccg gcgtggcgcg gaagaccgcg aacgtcgtcc
     3181 tcggcgtcgg gcacggcatc gccgaggggg tcgtcgtgga cacgcacgta tatcgcgtgt
     3241 cgcgccgcct gggcctgacc cggggcaagg gcccggtgga ggtcgagcag gacctcatgc
     3301 agacgatccc gcgcgacaag tggatcgagt tcgcgcatct cttgatcttc cacgggcgcc
     3361 gcgtctgcgt ggcgcgcaag ccgcgctgcg aggcgtgcgc ggtgctcgac ctgtgtccga
     3421 gcgcgtccta tttcctggcg ggaaaggtgc cgccgtggga tcggggtggc gaggccgggg
     3481 agcctcgcgc ggcgaagaag ggtccgcggt cgcgggtcgc cgccaaggtc gccgcgggcg
     3541 ggaaggccac ggcgcgtaag cgggcggtcc cgaagcccgc ggcgtcacgc aaggcacgga
     3601 agtcctcggc gccccggaag ctccgaaagt ccgccgcggc gcgcgcccgc cggagagcct
     3661 cgtgaacccc ggtgccgggc tccccgggac ggtcgcgccc gacttcgacg tccgcctgct
     3721 ggaagggggc taccaggagc tgcgcgacct ggtgcagccc gggggcggca tcgtcatctt
     3781 cttcaagacg gagtgcgaga ccagcgcgct cctcctggag cgcatcggcc cgctggcccg
     3841 ggcgctcgag ggcgaggagc gcgtcttcct ggccgtggcg cagaatcccg ccgcggaggt
     3901 gcgcgccttc cgcgagcagc accggatcgc gttcccgctc gcgtgcgagg aaccgccgta
     3961 ccccgcttcg cgcgcgtacg ccgtcgtcac cgtgccgaca ctgatcgtca tcgacggcac
     4021 gggaaggatc gccgagcgcg tggagggatt cgtcaaatcc gaagtcctgg ccctcgggcc
     4081 ggccgtcgag caggcgctcg cgctggggga catccccccc gtgctcgacc gccccgacga
     4141 gctgcccgag ctcaagcctg gctgaacctc gaaaacgacg gccgccgggt agcggccgag
     4201 gagaggtcat gaacgaaacg aatggaatcg ccatcacctg gctcggccac gccaccgtgc
     4261 tctatcgctc gcctcgcggc aagcaggtcc tggtggatcc ctggatcgaa gggaatcccg
     4321 cgagcccccc ggaggcgaag aagctctccg gagtcgacgt gatgctgatc acgcacggcc
     4381 acggcgacca cttcgccgac gccatcccgc tcgcgaagaa gttccatccg gagatcgtct
     4441 gcaactacga gatctccctg ttcctggaat ccaagggcgt gaaggagaag gtgcacggca
     4501 tcaacaaagg cggctcggtc accgtcgccg ggatccgggt caccatggtc cacgccatcc
     4561 actccagcag catcgaggat ggcgaccgga tcgtcccggc cggcgagccg tgcgggttcg
     4621 tgatcgaatt cgaggacgga acgcgcgtct accacgcggg ggacaccggc ttcttcggcg
     4681 acatgaagtg gatcggcgac ctttacaagc cctcgatcgc ggcgcttccg atcggagacc
     4741 tcttcacgat ggccccgcgc gaggcggcga tcgcggcccg gctgatcggc gcggcgcacg
     4801 tgctgccgat ccatcactcg acctttccgg ccctcacggg aacgccggcc gagctgaagc
     4861 gccagctggc cgaccagagc gagatccacg tgatcgaggt gaagccggga gagacggtcg
     4921 agccggagct ggcgcggcgc agctagccgg agctggcgcg gcggggctag cgagttaccc
     4981 gcgccagcgc gggtgaagtc gctgaccgcc gtcgttccgg cggacggtca atcgaagtag
     5041 acccgctcca ggacgcccgc tcgcgtgacg cggaagggct ggcgcgcgcg gaagtgctcg
     5101 accgccgaga gcgtgccgtc cttggggcgg atcgcgtcgc gcgcgatcca ggcgcgcagc
     5161 cacacggcga tctcgtccgc ttcgggatgg ggatgcgggc gcagcgaggc cccttccacg
     5221 cggccgtcgg gctcgcgcgt cagcgtgcag ccgcaggtca cgggaacggc gatcccgctc
     5281 agggatccga gggtgcgatc gcgcaccacg acgtgatcgc gatggatgag ctgctcgcgc
     5341 acgccgccgc ctacgcgagg ggaccaggtc cgccgctcga ggcgggctcc gcgcggaagg
     5401 cccagcgccg cccggatggc ggcggcgccc ccggcgcgat cgggatcgag cgcgcgcagc
     5461 gatcgatagc ttccgcgggt cccggcccgc gtgtcgatcc ttccgcgcac ggtcgccgtc
     5521 tttgggcgag gcgacggcag cttgatcccg tgcttcgcga acgcctcctc gatcccggcc
     5581 gacgcgacct cccctccttc cgtctggtcc acctcccgca tccgctgcgc gaccgcccga
     5641 tagtgccccg cgcccggagg cagcgtctcg agggcgcgag cgagcagccg gccacccagg
     5701 cggcgcgccc gctccaccgc gggctccggc tcctcggggg tctcgcgcag cgcgcgggcg
     5761 atcagccagg ccaggagatc gtagaaagcg cccgtgaaga cgcgggagaa ggagtgcggc
     5821 tcggacgaca gcttgtcgtc ggaggcaccg gccgggagcg tcgtgggcgg gcggtagcga
     5881 aacgcgttgt tcgcgtcccg caggcgcgtg gggtcggaga ccgcccgcgg accgtagttg
     5941 tcgtacacgg cgcgtcccag cgcctcggcc aggcgcgaga ccaggttgct cttccggatc
     6001 gccgcgcccg cctcgtcgag gaaccggcgc cgcaccgccg gctcggtcag cgtgacgagg
     6061 agcgacgagc agtcggcgaa cgcctcgtgg aatgccgcga tctcggggtc ggcgctggac
     6121 cagaacgcag gctggtagac gtcgagcacg gcgtgtcccg tctcgtgcgc caccacgtcg
     6181 aggctctcgc aggcgtagac cgcgcgcccg gtcacccggt cggtgtcgta gaagaactgg
     6241 aggctcgcgc ggtcgtagaa agcgttcagg tcgcgccccg cgcgaagccg gacgcggaga
     6301 ggatgcccgt cgtgccacgc cgcgaagtcg cgatcgaaga gatcctccca gacgcggatc
     6361 gtccggcgga gcgcggtctc cccctgccag tactggaagg cgagcgtgcc cggatcgtac
     6421 ggcgcggggg cgtagcggcg gccgtcgatc acgtaggagg gatcgacgtc cagcggcgcg
     6481 atcggcgtcg cggggacgag tccgaccgag gcctcgggat cgttcgggaa gatccggatg
     6541 tcgctggagg cgcggcgccg gggtgccgag gcgcggcggg ccttcgccga ggcgccgccg
     6601 ccgcggcggg ccgggcgcgc cgcgcgagcg cggccgcgct tcatcgccgt gcgcggaaaa
     6661 gctcgagcgc gccctcgacg gggagcgtgt tcacgacgtc gcccggaccc agccatccct
     6721 tgcgcgcgac gccgattccg aggcggagat cctggtagcc gctcacgtgg tgcgcgtcgg
     6781 ggtcgatggc gaacttgacc ccgagcgcct tgccggcggg gatctcgcgc cagtcgaggt
     6841 cgaggcggcg cgaatgggcg ttgatctcga ccacgacgcc ctcctgggcg gcgacgcgga
     6901 agacttcgtc gaagcggatc cggtacccct cccgctggag gagcagccgt ccggtgggat
     6961 gcgccaggat gctcacgtgc ttgttccgca gggccttcac cacgcgcgcg gtcatttcgt
     7021 cctcgcccat gttcagattg gtgtggaccg acgccacgac gaactcgagc cgctcgagca
     7081 cccggtcggg gtagtcgagc gttccgtccg gcatgatgtc gacctccgct cctttcagaa
     7141 tacggaggtc gtgcgacttc gagttccact tgtcgatttc cttggcctgg cggagcaggt
     7201 cctcctcgga aagtcccccc gcgtagcgca cgctctggct gtggtcggag atgcccaggt
     7261 attcggcgcc cgcggccacg gccccctccg ccatctcctc cagcgtggac ttgccgtcgc
     7321 tgtaggtggt gtggcagtgg aggatcccct tgaggtcgcg ggcggtgatc aggccgggaa
     7381 ggcggcccac ctcggccgct tcgatctcgc cgcgatcctc gcgcagctcc ggctccacgt
     7441 aggccatgcc gagaacctga aagagatccg cctcgctcgc gatcggcgcg tgctcgccac
     7501 ccgagagctc gtattcgttg aggctgaagc cgcgctcgat cgcgcgctga cgcacgcgca
     7561 cgttgtgcgc cacgctcccg gtgaagtagt ggagcgcgaa ggggaactgc tcgttcgtga
     7621 ccagccggag gtccacggcg agcccggtgg ggaggcgaac ggacaccttc gtgtctccct
     7681 gggcggtgat cgaggcctgg gggagcagct tgaggaacgc tttcgccacc gcgtccggct
     7741 tcttcgtcgc caccaggaga tccacgtcct tcacggtctc cagccagcgg cgcaggctcc
     7801 cgcccagctc ggcgcggatc acgtccttcc gatccctgag cgcggccagg atctcctcgg
     7861 cgatggggcg gacgaccgcg gccagctcgc gccggccgcc gcgctccagg aaagcgatcc
     7921 cctcgaggat cttcttctcc gactgctccc cgaatccctt caggccgcgg atccgccctc
     7981 cttcggcggc ctccttcagg gcttcgaccg acgcgatctg aaggaggtcg cagagggtgc
     8041 ggatccgctt ggggcccagc cccgggatcc gcatcatgtc gaaccacccg acggggaccg
     8101 tctcgcagag gtcctcgaag tacttcatct tcccggtggt ccagagctct tcgacgttct
     8161 tctggatgga ggagccgatc ccgcgcactt cggagaggcg cttgtcggcg atcagggtcc
     8221 cgaggtcttc ggagagggca tcgacggcgc gggcggcgtt ttcataggcg cgcaccttga
     8281 aggggttctc ccccttgatc tcgaggatcg cggcgatctg ctcgagggtg cgggcgacgt
     8341 cgcgcttgtc caagggctag aacttgcggc ctgggccggg gttttggagg tcagcgcagc
     8401 tcgtcgagcg ggcggcgtcc ggagaagcct tcgtccagcg ggtgccagtg ctcgatccgc
     8461 ggctcccccc ggcgccagca gaggcagacg atctgctccc ggtgcatcgt gtagaagtcg
     8521 accaggccca gctcgaggtc gcggacgatg cagccgtgcc gggagacctc ggccaggacg
     8581 gagcggaagc gctccaccgt ggtttcgtag gcatcctcct tctcccccag cgaccggaga
     8641 tcggggttca gatcgctggc ccccgagctg gcgatggcgc ggaggacgtc gatctcgcgc
     8701 ttca
//
LOCUS       DASSLZ010000292         4132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1501353, whole genome shotgun sequence.
ACCESSION   DASSLZ010000292 DASSLZ010000000
VERSION     DASSLZ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501353"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /gene="ftsH"
                     /locus_tag="VFM00_11020"
     CDS             complement(<1..1758)
                     /gene="ftsH"
                     /locus_tag="VFM00_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7498822.1"
                     /translation="MTARSRDDSRPAQKNSERRERKRPPNPPRQPQRKPTPFGPARPL
                     RTALLWVILVLAVLVFVQVYGEIKAKTHEIPYSEFLGQIDGANIKAVTIVEREVVGEL
                     KQSALTHVDGRPVSYVYFKVYLPAEDKDLGRAILAKDASATVTSKPPGMNWWALVISY
                     IPFVALVVLWMVMIRQIQAGGASAMKFGKSRPKVALESSPKVTFKDVAGADDAKQELE
                     EIIEFLRDPKKFQRLGGRIPKGALLLGPPGTGKTLLAKAVAGEAGVPFFSLSGSDFVE
                     MFVGVGASRVRDLFDQGKRNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVE
                     MDGFESNDGVILIAATNRPDVLDPALLRPGRFDRQIVVDVPDVRGREGILRVHTRNIP
                     VSEEVNLTVLARGTPGMVGADLANLVNEAALLAARRNHKKVNSVDFEDAKDKVMMGAE
                     RRSLVISDEEKKSTSYHEAGHALVAWLQPGSDKVHKVTIIPRGRALGLTAYLPEEERH
                     SFSREYWLRMLTHLMGGRAAEQLVLNHFTTGASDDIRRATMIARRMVCEWGMSERLGP
                     LTFGTREEYVFLGKEISQHR"
     gene            complement(1835..2938)
                     /gene="tilS"
                     /locus_tag="VFM00_11025"
     CDS             complement(1835..2938)
                     /gene="tilS"
                     /locus_tag="VFM00_11025"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET7498823.1"
                     /translation="MPADGTLGKPLNPRDRTLDRIAARRVRADRLVPEDGSVLAAVSG
                     GADSVALLHFLATDRERTGRPASLAVGHVNHGLRGRHSDEDAAFVRDLAARLALPYFE
                     EMVPTGVLRPAGAAASEPGSLASEDLARRVRYEALDRLAALAGAAVVATAHTADDQTE
                     TVLFRMARGAGLRGLSGMRSRARVHGVRVIRPLLDVTREQVLAYLAFHGHAHREDASN
                     ANLGAARNFLRHEILPRLRDGVNAGVRDALLRQADLFRELDGYLEAEARRVLPEVTLS
                     AEEGKIVLDADRLVSYPKLLRSYIFRCALNDLDGVAREVLATHVDVLHFLANDRRGRS
                     ADFPMGVRVRRERGRIILTAGKENRPEATSIQH"
     gene            complement(2938..3711)
                     /locus_tag="VFM00_11030"
     CDS             complement(2938..3711)
                     /locus_tag="VFM00_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498824.1"
                     /translation="MGSDRATSRDHEATSKVPAVTLLFWVIKIAATTLGETGGDAVTM
                     SLHLGYLIGTIVFAALFVVMVMAQIRASRFHASIYWATIIATTTVGTTVADLVDRDLG
                     IGYAGGSTLLLAILLTSLFLWKRTLGTVSVDSVSTPAAEWFYWVTILFSQTLGTALGD
                     WTADTASFGYGGSAIIFGSLLAIVAAGYLWTRISRTALFWAAFILTRPLGAVLGDLLD
                     KPVSKGGLALSRFGASAVLLFVIVTCILAFRQRPARAAH"
     gene            complement(3756..>4132)
                     /locus_tag="VFM00_11035"
     CDS             complement(3756..>4132)
                     /locus_tag="VFM00_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498825.1"
                     /translation="IVETYRNVIPDRGLTLTELDVTAVEENPATPAPPFSLAAPAPNP
                     VRDEAAFRFGLPLAARVRLDLFDIRGRRVATALDAQLPAGRHEAVWRRTDARGRRVAS
                     GVYVARLEALGEVRIRKLVLVR"
BASE COUNT          661 a         1390 c         1459 g          622 t
ORIGIN      
        1 gcggtgctgc gagatctcct tgccgaggaa gacgtactcc tcgcgcgtgc cgaacgtgag
       61 gggacccagg cgctcgctca ttccccactc gcagaccatc cgccgcgcga tcatcgtggc
      121 gcgccggatg tcgtcggagg cgcccgtcgt gaagtggttc aggacgagct gctcggcggc
      181 gcggccgccc atgaggtgcg tcagcatccg gagccagtac tcgcgcgaga aggaatgccg
      241 ctcctcctcc gggagatagg ccgtgaggcc cagcgcgcgg ccgcgcggga tgatcgtgac
      301 cttgtgtacc ttgtcgcttc caggctgcag ccaggccacc agcgcgtgac ccgcctcgtg
      361 ataggaggtg ctcttcttct cctcgtcgga gatcaccagg ctgcgccgct cggcgcccat
      421 catcaccttg tccttggcgt cctcgaagtc gacggagttg accttcttgt gattgcgccg
      481 cgccgccagc agggccgctt cgttcacgag attcgcgaga tcggccccca ccatgccggg
      541 cgtgccgcgc gcgagcacgg tcaggttcac ctcttcggag accgggatgt tccgggtgtg
      601 gacgcggagg atcccctcgc gcccgcggac gtccggaacg tccacgacga tctgccggtc
      661 gaagcgcccg ggccggagca gcgccgggtc gagcacgtcg gggcggttcg tggcggcgat
      721 caggatcacg ccgtcgttcg actcgaagcc gtccatctcg acgaggagct ggttcagcgt
      781 ctgctcccgc tcgtcatgcc cgccgcccag tcccgcgccg cgatggcggc cgacggcgtc
      841 gatctcatcg atgaagatga tgcagggggc gttgcgcttc ccctggtcga agaggtcgcg
      901 cacgcgcgag gcgccgacgc cgacgaacat ctcgacgaag tcggacccgg agagcgagaa
      961 gaagggaacg cccgcctcgc cggccaccgc cttggcgagg agggtcttgc ccgtgcccgg
     1021 aggaccgagc aggagcgcgc ccttcggaat ccgtccgccc aggcgctgga atttcttcgg
     1081 gtcgcgcagg aactcgatga tctcctccag ctcctgtttc gcatcgtccg cgcccgcgac
     1141 gtccttgaac gtgaccttgg ggctggactc gagcgcgacc ttggggcggc tcttcccgaa
     1201 cttcatcgcc gaggcgccgc ccgcctggat ctggcggatc atgaccatcc agagaacgac
     1261 gagcgcgacg aatgggatgt aggagatgac gagcgcccac cagttcatgc ccggcggctt
     1321 ggacgtgacc gtggcggacg cgtccttcgc gaggatcgcg cggcccaggt ccttgtcctc
     1381 cgccggcagg tagaccttga agtagacgta ggagacggga cgtccgtcca cgtgggtcag
     1441 cgcgctctgc ttcagctcgc ccacaacctc gcgctcgacg atcgtcaccg ccttgatgtt
     1501 cgcgccgtcg atctgtccca ggaactcgga atagggaatc tcgtgggtct tggccttgat
     1561 ctctccgtag acctgcacga agacgagcac ggcgaggacc aggatcaccc agagaagggc
     1621 ggtcctgagt ggccgcgccg ggccgaacgg cgtcggcttg cgctgcggct gtcggggagg
     1681 attgggcgga cgcttgcgct cccggcgctc ggagttcttc tgagcggggc gcgaatcgtc
     1741 gcgcgaacgc gcggtcaagg ggctcaattg ctccggattc gggttcactc ggctggactt
     1801 cctttcctgg aatcggcaga gatgccggat gtctctagtg ttggatggaa gttgcttcgg
     1861 gccggttctc ctttccggcc gtcaggatga tccgcccgcg ctccctgcga acccgtacgc
     1921 ccatcgggaa atcggccgag cggccgcggc ggtcgttcgc gaggaaatgc agaacgtcca
     1981 cgtgggtcgc caggacctcc ctggccaccc cgtccagatc gttcagggcg cagcggaaaa
     2041 tgtacgaacg caacagttta gggtacgaaa cgaggcgatc ggcatcaagc acgattttcc
     2101 cctcctcggc gctcagcgtc acctcgggga gcacgcgccg tgcctcggcc tccagataac
     2161 cgtccagctc gcgaaaaaga tccgcctggc ggagcagcgc gtcccgtacc cccgcgttca
     2221 cgccgtcgcg gaggcgcggc aggatctcat gccggagaaa gttgcgtgcc gccccgagat
     2281 ttgcgttgga ggcgtcctcg cgatgcgcgt ggccgtggaa cgccagatag gccaggacct
     2341 gttcccgggt gacgtcgagc agggggcgga tgacgcgcac gccgtggacc cgggcccgcg
     2401 atcgcatccc ggacaggccc cggagaccgg ccccgcgcgc catccggaac agcactgtct
     2461 ccgtctggtc gtcggcggtg tgggcggttg cgactacggc cgctccagcc agggccgcca
     2521 ggcggtcgag cgcctcgtag cgaacccggc gagcgagatc ctcggaggcc aggcttccgg
     2581 gctcggacgc ggcggccccg gcgggccgaa ggaccccggt cgggaccatc tcttcgaaat
     2641 agggcagggc gagccgtgcg gcgaggtccc tcacgaacgc ggcatcctca tcggaatggc
     2701 gcccgcgcaa gccgtggttg acgtgcccca cggcgaggga ggcgggacgg cccgtccgct
     2761 cgcggtccgt ggccaggaaa tggaggagcg ccacggagtc ggcgccgccc gagacggcgg
     2821 caagcacgct tccgtcctcg ggaacgagcc ggtcggcgcg gacgcgccgc gccgcgatcc
     2881 ggtccagcgt gcggtcgcgc ggattgagcg gcttcccgag cgtgccgtcg gcgggcatca
     2941 gtgcgccgcc cgggccgggc gctgccggaa cgcgagaatg cacgtcacga tgacgaagag
     3001 cagcaccgcg gaggccccga agcggctcag cgccagccct cccttgctca ccggcttgtc
     3061 gagcaggtcg cccaggacgg cgcccagcgg gcgcgtcagg atgaacgcgg cccagaagag
     3121 cgccgtgcgc gagatgcgtg tccagagata gcccgcggcg acgatggcga gcagcgagcc
     3181 gaagatgatc gcgctcccgc cgtatccgaa gctcgccgtg tcggccgtcc agtcgcccag
     3241 agcggtcccc aatgtctgcg agaagaggat cgtgacccag tagaaccact cggccgccgg
     3301 cgtgctgacg gaatccaccg agaccgtgcc gagggtgcgc ttccagagga agagcgacgt
     3361 gagaaggatg gccaggagca gggtcgagcc gcccgcgtag ccgatcccga ggtcgcggtc
     3421 gacgaggtcg gccaccgtgg tcccgaccgt ggtggtcgcg atgatggtcg cccagtagat
     3481 ggaggcgtgg aagcgcgagg cccggatctg ggccatgacc atcacgacga agagcgcggc
     3541 gaagacgatg gtcccgatca gatagccgag atgaagcgac atcgtgaccg cgtcgccgcc
     3601 ggtctcgccc agcgtcgtcg ccgcgatctt gatcacccag aagaggaggg tgaccgccgg
     3661 caccttgctg gtcgcctcgt ggtccctcga tgtcgcgcga tccgagccca tcgcaaaatt
     3721 gtacggctcg ggagcaggat gtctagcgaa cccgcctagc gcacgagaac cagtttccgg
     3781 atccggacct ccccgagggc ctcgaggcgc gccacgtaaa cgcccgaggc gacgcgcctt
     3841 cctcgcgcgt cggtgcgccg ccagacggcc tcgtgacgcc ccgcggggag ctgggcgtcc
     3901 agcgcggtcg cgacccgccg gccgcggata tcgaacagat cgagccggac gcgggcggcc
     3961 aggggcagcc cgaagcggaa ggccgcctcg tcgcgcacgg gattgggcgc gggggccgcg
     4021 agggagaagg gaggcgctgg cgtcgccggg ttctcctcca ccgcggtcac gtccagctcg
     4081 gtcagcgtga ggccgcggtc cggaatcacg ttccggtagg tttcgacgat gc
//
LOCUS       DASSLZ010000293         6588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_107926, whole genome shotgun sequence.
ACCESSION   DASSLZ010000293 DASSLZ010000000
VERSION     DASSLZ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107926"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFM00_11040"
     CDS             <1..443
                     /locus_tag="VFM00_11040"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly(A) polymerase"
                     /protein_id="HET7498826.1"
                     /translation="FSRDLTDRVAHLVKNHMFDYRPEWTDATVRRFVRTVGIENVADL
                     FDLRIADNLGNGLKTGFPHYLGELTERIGRVVEAQEALSVRDLKVDGADVMRELGLAP
                     GPDVGRILDRLLEEVTEDPSLNDRKRLLRRLSQGMAIDTAGPPA"
     gene            490..1509
                     /locus_tag="VFM00_11045"
     CDS             490..1509
                     /locus_tag="VFM00_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET7498827.1"
                     /translation="MTRTSQSPARPNPEEPTLASIREPIAEAFERFQERLDDAFLCDV
                     SLVGAVGAHLHRAFGKRFRPTLLLLATRGFGDISDESVLGAVVVELIHTATLIHDDSV
                     DKSLVRRGLPTVNSAWNNDVAILMGDYLYSKAFSILVKNRMYEAMDLLAEATHRMSQG
                     ELRQIEQKNRLDLTEAEYMRVIEEKTACLISAGCEFGAFSGGAAPEARQAMFRYGRAV
                     GLAFQITDDIFDYLGAENVMGKAMGSDLEGGKITLPLITALRNASDPARREMRAMIQN
                     REFRNGHWGDVVRFVTENGGVEVSEERSSALAREAEAELDHVKDDVARTALTDAVRYA
                     VTRRR"
     gene            1506..1898
                     /gene="rsfS"
                     /locus_tag="VFM00_11050"
     CDS             1506..1898
                     /gene="rsfS"
                     /locus_tag="VFM00_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET7498828.1"
                     /translation="MKQPEPEPEPREAQRHSDSQALARAAARLTLTKRAQDVMILDLR
                     ELDGVCDFFVLATGSSEVQVRAIADAVDDGLRAEGMKPWHVEGYEARRWILLDYVDVV
                     VHVFHAKAREYYLLDKLWGDARRELVAD"
     gene            1879..3549
                     /gene="argS"
                     /locus_tag="VFM00_11055"
     CDS             1879..3549
                     /gene="argS"
                     /locus_tag="VFM00_11055"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET7498829.1"
                     /translation="MNSWPIEAYLTERLAAAVGKAGFTLPEGTAVEVEVPREESHGDW
                     ASAVALSLAKAARRPPREVANAIVAALDVEDGVLGKVELAGPGFINFRLASPWLQDAV
                     RRILADPDRYGASESGRGERILIEYVSANPTGPLNIVSARAASFGDSLIRILNAAGYR
                     ADGEFYVNDSGLQAELFGASVRTRYCEARGLPAPEIPEEGYAGSYVAEVAARLDERQA
                     SAWLKLPEREQRAAFGAAAIDLMVERQRAQLEQFGVRMERWFRESELHREGRVQAELE
                     RLRASGHVYEKDGAVWFRSTSFGDQEDRVIVRSNGIPTYFLPDIAYHDDKLSRGYSRA
                     LDLWGPDHHGHIQRMKAALQALGYGPERFDVITLQWVKLLRGGEIVKMSKRAGEFVTM
                     EELVEEVGVDAARFFFLMRRAESPMDFDLELAVKRSEENPVYYVQYAHARIFHVLEYA
                     HHQGVAEPDLAQARLELLCEPETETLLRALASFPSLLAAAARTREPHRIPQYLKDLAA
                     RFHAFYHFHRVVGTDPDTTAARLLLTRATGVVLKRGLALMGVSAPEAM"
     gene            3549..4478
                     /locus_tag="VFM00_11060"
     CDS             3549..4478
                     /locus_tag="VFM00_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7498830.1"
                     /translation="MGIAVVGSLGLDTIETPTGRVEEVLGGSAAYFTLAARHFTPVHV
                     VAVVGSDFPAEARAALTLPDVDLSGLEVAPGRTFRWEGVYSEDLNTRRTLRTELNVFE
                     HFRPELPAAVRRLRDVFLANIDPVLQRDVLAQLVSPRLVLADTMNYWIANKREELLET
                     LKKVRILLINEEEARELTGESLTHKAARAILALGPETVVVKMGEHGAFLLSRNEYFIC
                     PAFPLETVVDPTGAGDTFAGGFMGALTRMGRITDTNLRRAVVYGCVLASFAVEEFSVG
                     ALLRVERGEVLRRAEAITAMTRVSLDNTARVLR"
     gene            4708..6069
                     /locus_tag="VFM00_11065"
     CDS             4708..6069
                     /locus_tag="VFM00_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7498831.1"
                     /translation="MHVTTSLPSSTAFARSRSIAARVVWFVVPSFLAALPVLLALFGG
                     PEVKQTRPDLVGVVVWFVLLVVAESHPVPLPRGGTITIASVLDVGAILLFGPWVAGAL
                     DLLTAIPAQLFLLRERPAAAVLNGGIYASTTILAGSAYLAAGGALGAPRFQDFLPILI
                     AGALYYAVNTGWVSLLVGAHTRESPRTIWRQQFRDGLLQHALAIGFGLLFAQVRVVAG
                     IPGVLLLTVPLFLARYALQLYADLREDLVGFVRALSTALDAVDPYTHRHSVRVAGYSV
                     RVARHLALPESEVEMIHYAALVHDIGKIAQRPDVIRKPTPLTEEERLLMMHHPEAGAK
                     IVEQIRALRAAAEMVRTHHWRPDGEGYPSGLTEAEVPLGARIIHVCDAFDAMTSDRSY
                     RRGLPVACALAELSAKAGTEFDEDIVAALVQLHEAGVLEDVRGAGERADEFFLPESSP
                     EPR"
     gene            6066..>6588
                     /locus_tag="VFM00_11070"
     CDS             6066..>6588
                     /locus_tag="VFM00_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HET7498832.1"
                     /translation="MSRYEDAGVNIRKGEEAVARIRDLCRSTFGPDVLGDVGLFAGAY
                     RVPGMPGQVLLSSIDGVGTKLKVAILCDRHDTVGYDLVCHSANDILVHGATPLFFLDY
                     IAMGALDPVRVERLVEGLARGCREVGCALIGGETAEMPGLYAPGDYDLAGAIVGIAAE
                     RDLIRGEGIAPGDV"
BASE COUNT          945 a         2337 c         2301 g         1005 t
ORIGIN      
        1 gcttctcccg cgacctgacc gaccgggtgg cgcatctcgt gaagaaccac atgttcgact
       61 accgtccgga gtggaccgac gccacggtgc gccgcttcgt ccgcacggtc ggcatcgaga
      121 acgtggccga tctcttcgac ctccggatcg cggacaacct ggggaacggc ctcaagaccg
      181 gcttcccgca ctacctgggc gagctgaccg agcggatcgg acgggtggtc gaggcgcagg
      241 aggccctctc ggtgcgggat ctcaaggtgg acggcgcgga cgtgatgcgc gagcttgggc
      301 ttgcgccggg tcccgacgtg gggcgcatcc tggaccggct gctcgaagag gtgaccgaag
      361 acccctcgct gaacgaccgg aagcggctcc tgcgccgcct gtcccagggc atggcgattg
      421 acacggcggg ccctcccgcc tagactggcg cgtccgcaag gaacgggccc ccatgaaact
      481 aggagtgaat tgacccgaac gagccagagt cccgcgcggc cgaaccccga agaacccacg
      541 ctcgcctcga tccgagagcc gatcgccgag gcgttcgagc gctttcagga gcggctcgac
      601 gacgcctttc tctgcgacgt ctccctcgtg ggcgcggtcg gcgcgcatct ccatcgcgcc
      661 ttcggaaagc gcttccgccc cacgctcctg ctgctcgcca cccgcggctt cggcgacatc
      721 agcgacgagt cggtgctggg cgcggtggtc gtggagctga tccacacggc cacgctgatc
      781 cacgacgaca gcgtcgacaa gagcctggtg cgccgcggcc tgcccacggt gaacagcgcc
      841 tggaacaacg acgtcgcgat cctcatgggc gactacctgt attccaaggc gttctccatc
      901 ctggtcaaga atcgcatgta cgaggcgatg gatctcctgg ccgaggcgac gcaccggatg
      961 agccagggcg agctgcgcca gatcgagcag aagaaccgcc tggacctgac cgaggcggag
     1021 tacatgcgcg tcatcgaaga gaagaccgcc tgcctcatct cggccggctg cgagttcggg
     1081 gccttctcgg gcggggctgc tcccgaagcg cgccaggcga tgttccgcta cggccgcgcc
     1141 gtcggcctcg cgttccagat caccgacgac atcttcgact acctgggcgc cgagaacgtg
     1201 atggggaagg cgatggggag cgacctcgag ggggggaaga tcacgctgcc gctcatcacc
     1261 gcgctccgga acgcctccga tccggcgcgc cgggagatgc gcgcgatgat ccagaaccgc
     1321 gagttccgga atggccactg gggcgacgtg gtccgtttcg tcaccgaaaa cggaggcgtc
     1381 gaagtcagcg aggagcgctc ttccgcgctg gcccgcgagg ccgaggcgga gctggaccac
     1441 gtgaaggacg acgtcgcccg cacggcgctg accgacgcgg tccgctacgc cgtgacgcgc
     1501 cgccggtgaa acagcccgag cccgagcccg agccgcgcga ggcgcagcgg cactccgatt
     1561 cccaggccct ggcgcgcgcg gccgcccgcc tcacgctgac caagcgcgcc caggacgtga
     1621 tgatcctcga cctgcgcgag ctggacggcg tctgcgactt cttcgttctg gcgaccggga
     1681 gctccgaagt ccaggtgcgc gccatcgcgg acgccgtgga cgatggactc cgcgccgagg
     1741 ggatgaagcc ctggcacgtc gagggctacg aggcgcgccg ctggatcctc ctcgactacg
     1801 tggacgtcgt cgtgcacgtc ttccatgcca aggcccgcga gtactacctc ctcgacaagc
     1861 tctggggcga cgcccgacgt gaactcgtgg ccgattgaag cctacctgac cgagcgcctc
     1921 gcggcggcgg tcgggaaggc gggattcacg ctgcccgagg ggaccgcggt cgaggtcgag
     1981 gtgccgcgcg aggagagcca cggcgactgg gcctccgcgg tcgcgctctc cctggccaag
     2041 gccgcgcggc gcccgccgcg cgaggtggcg aacgccatcg tggccgcgct ggacgtcgag
     2101 gacggcgttc tcggcaaggt ggagctcgcc gggcccggct tcatcaattt ccgcctcgcc
     2161 tcgccgtggc ttcaggacgc ggtgcgccgc atcctcgccg acccggaccg ttacggcgcc
     2221 tcggagtcgg ggcgcggcga gcggatcctc atcgaatacg tcagcgccaa ccccaccggc
     2281 ccgctcaaca tcgtgagcgc gcgcgccgcc tccttcggcg actccctgat ccgcatcctg
     2341 aacgcggcgg gctaccgcgc cgacggcgag ttctacgtga acgactcggg cctccaggcc
     2401 gagctcttcg gcgcctcggt gcgcacgcgc tattgcgagg cgcgggggct gcccgcgccg
     2461 gagatccccg aggaagggta cgccggctcc tacgtggccg aggtggccgc gcggcttgat
     2521 gagcgtcagg cgtcggcatg gctgaagctt ccggagcggg agcagcgcgc cgccttcggc
     2581 gcggcggcga tcgatctcat ggtcgagcgg cagcgggcgc agctggagca gttcggcgta
     2641 cggatggagc ggtggttccg cgaatcggag ctccaccggg aggggcgcgt tcaggccgag
     2701 ctggagcggc tgcgcgcgag cggccatgtc tacgagaagg acggcgccgt ctggttccgg
     2761 tccacgtcgt tcggcgatca ggaggatcgg gtgatcgtcc gctcgaacgg catcccgacc
     2821 tacttcctgc ccgacatcgc ctaccatgac gacaagctct cgcgcggcta ctcgcgcgcg
     2881 ctcgacctct gggggcccga ccatcacggc cacatccagc ggatgaaggc ggccctccag
     2941 gcactgggct acggccccga gcgcttcgac gtgatcacgc tgcagtgggt gaagctcctg
     3001 cgcggcgggg agatcgtgaa gatgtccaag cgggccggcg agttcgtcac catggaggag
     3061 ctggtggagg aggtcggcgt cgacgccgcc cgcttcttct tcctcatgcg gcgcgcggaa
     3121 agtccgatgg acttcgacct ggagcttgcc gtcaagcgga gcgaggagaa cccggtctat
     3181 tacgtgcagt acgcgcacgc ccggatcttc catgtgctcg agtacgccca tcaccagggg
     3241 gtggccgagc ccgacctggc tcaggcccgc ctggagctct tgtgcgagcc cgagaccgag
     3301 acgcttctcc gcgcgctcgc ctcgttcccg tcgctcctcg ccgccgcggc acggacccgg
     3361 gagccccatc ggatccccca gtatctcaag gacctggcgg cccgctttca cgccttctat
     3421 cacttccatc gcgtggtcgg gaccgatccc gatacgaccg cggcgcgcct gctcctcacc
     3481 cgggcgacgg gagtcgtgct gaagcgcggt ctcgccttga tgggcgtgag cgcgccggag
     3541 gcgatgtagt gggcatcgcg gtcgtcggtt ccctgggtct cgacaccatc gagaccccga
     3601 ccgggcgcgt cgaggaggtg ctcggcggct cggccgccta cttcacgctg gccgcgcggc
     3661 acttcacgcc cgtccatgtg gtggccgtgg tcggctccga ctttccggcc gaggcgcgcg
     3721 cggccctcac gctgcccgac gtcgaccttt cggggctcga ggtggcgccg ggacgcacct
     3781 tccggtggga gggggtctac agcgaggatc tgaacacgcg ccgcacgctg cgcaccgagc
     3841 tcaacgtctt cgagcacttc cgccccgagc tgcccgccgc cgtgcggcgg ctgcgcgacg
     3901 tctttctggc gaacatcgac ccggtcctcc agcgggacgt gctcgcccag ctggtgagcc
     3961 cgcggctggt cctggcggac accatgaact actggatcgc gaacaagcgg gaggagctcc
     4021 tcgagacgct gaagaaggtc cgcatcctcc tgatcaacga ggaggaggcg cgcgagctga
     4081 cgggcgagtc gctcacgcac aaggcggcgc gcgcgatcct ggcgctcggc cccgagacgg
     4141 tggtcgtgaa gatgggcgag cacggcgcct tcctcctgag ccggaacgag tacttcatct
     4201 gcccggcctt cccgctcgag accgtcgtgg accccacggg cgccggcgac acgttcgcgg
     4261 gcgggttcat gggggcgctg acgcggatgg ggcggatcac cgacacgaat ctccgccgcg
     4321 cggtggtcta tggatgcgtg ctcgcctcgt tcgccgtcga ggagttcagc gtcggcgcgc
     4381 tcctgcgcgt ggagcgcggg gaagtgctgc gccgcgccga ggcgatcacg gcgatgacgc
     4441 gcgtaagcct ggacaacacg gcgcgcgtcc ttcggtgacc gcggcgccgc gcgcgatccg
     4501 gcacgcggcg cggatggcgc cggcgtgcgc gcgctccgac gccgacggcg tcccgacgcc
     4561 tccgacgcgc cgcgcggtcc gccctcacga cacccgatcg cgcgcccggc acgcgttagc
     4621 cctttatccg gacggcgcgt gctggcacac tcgttgcggc tcgccgaacc ctttcacaac
     4681 cgatatccac gaacgcgggg accaccgatg cacgtgacca cgtcgcttcc gtcctccacg
     4741 gcgttcgcgc gctcgcgctc catcgccgcg cgcgtcgtct ggttcgtcgt cccgtcgttc
     4801 ctggccgcgc tcccggtgct gctcgcgctc ttcgggggcc ccgaggtgaa acagacgcgg
     4861 cccgacctcg tgggcgtcgt cgtctggttc gtgctgctcg tggtcgccga gtcgcacccc
     4921 gtcccgctgc cgcgcggcgg caccatcacg atcgcttcgg tgctggacgt gggcgcgatc
     4981 ctcctcttcg gtccgtgggt ggcgggcgcg ctcgatctcc tgaccgccat cccggcgcag
     5041 ctcttcctgc ttcgggaacg gccggcggcc gccgtgctca acggaggcat ctacgcttcc
     5101 acgacgattc tcgccggctc cgcgtatctc gcggccggcg gcgccctggg cgcgccgcga
     5161 ttccaggact tcctcccgat cctgatcgcc ggagcgctct actacgcggt gaacaccggg
     5221 tgggtgagcc tgctcgtcgg cgcgcacacg cgggaatcgc cgcgcaccat ctggcggcag
     5281 cagttccgcg acgggctcct ccagcacgcg ctggcgatcg ggttcgggct cctcttcgcg
     5341 caggtgcggg tcgtggccgg catcccgggc gttctcctcc tcaccgtccc gctcttcctg
     5401 gcgcgctacg cgctccagct ctacgccgac ctgcgcgagg acctcgtcgg gttcgtgcgg
     5461 gcgctctcca cggcgctgga tgcggtcgac ccctatacgc accgccattc ggtgcgggtc
     5521 gcgggctatt cggtgcgcgt cgcgcggcat ctcgcgctcc ccgagtccga agtggagatg
     5581 atccactacg ccgccctggt gcacgacatc gggaagatcg cgcagcgccc ggacgtcatc
     5641 cgcaagccga cgccgctcac cgaggaggag cggctcctca tgatgcatca ccccgaggcg
     5701 ggggcgaaga tcgtggagca gatccgcgcg ctccgcgccg cggccgagat ggtccgcacg
     5761 catcactggc gacccgacgg cgaagggtat ccgtcgggac tcaccgaggc cgaggtgccg
     5821 ctgggagcac gcatcatcca cgtgtgcgac gccttcgacg cgatgacgtc ggaccgctcc
     5881 taccgccgcg gcctgccggt cgcgtgcgcg ctcgccgagc tctcggccaa ggcggggacg
     5941 gagttcgacg aggatatcgt ggccgcgctc gtgcagctgc acgaagccgg cgttctggaa
     6001 gacgtgcgcg gggcggggga gcgggccgac gagttcttcc tgcccgaatc gtcgcccgag
     6061 cctcgatgag ccgctacgag gacgccggcg tcaacatccg caagggtgag gaagcggtcg
     6121 cgcgcatccg cgacctctgc cgctccacgt tcggcccgga cgtcctgggc gacgtggggc
     6181 tcttcgcggg agcgtaccgg gtcccgggaa tgcccgggca ggtgctgctc tccagcatcg
     6241 acggggtcgg caccaagctc aaggtcgcga tcctctgcga ccgccacgat acggtcgggt
     6301 acgacctcgt ctgccattcc gcgaacgaca tcctggtcca cggcgccacg ccgctcttct
     6361 tcctcgatta catcgccatg ggggccctcg atccggtccg cgtcgaacgc ctcgtcgagg
     6421 ggctggcgcg cggatgccgc gaggtgggat gcgccctgat cggcggggag acggccgaga
     6481 tgccgggact ctacgcgccg ggcgactacg acctggccgg cgcgatcgtc gggatcgcgg
     6541 ccgagcggga cctgattcgc ggcgagggga tcgccccggg ggacgtcg
//
LOCUS       DASSLZ010000294         9688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1248766, whole genome shotgun sequence.
ACCESSION   DASSLZ010000294 DASSLZ010000000
VERSION     DASSLZ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9688
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248766"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFM00_11075"
     CDS             complement(<1..530)
                     /locus_tag="VFM00_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498833.1"
                     /translation="MSARGGTRASLALGAAGLAVALLAASRGGGARAADPVTTPAATT
                     AAPAPSTTATPAVAPAASTRHYPPRRAAELKARLLEAPDPEASPGWALEFAASGDVQR
                     LDPMTRAAEQLWAARIGLAGKATGSQRKELRSIVDRLESCPLDSVALKRAAQGIARVG
                     VIVPLSGRYERYGKTFV"
     gene            complement(520..1041)
                     /locus_tag="VFM00_11080"
     CDS             complement(520..1041)
                     /locus_tag="VFM00_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET7498834.1"
                     /translation="MIGPLLGSSPRVPASAFVAPNAVVLGDVLLGEQSSVWYGAVVRG
                     DAGPIRVGARSNIQDLCMLHATTGRSGLTIGDEVTVGHRVILHGAVVRDRCLIGMGSI
                     LLDDCEIGEESLVAAGAVVLEGMRVPPRSFVAGMPARIRGAIPPEALARLRESADVYV
                     ELAREHAKAIRER"
     gene            complement(1067..1582)
                     /locus_tag="VFM00_11085"
     CDS             complement(1067..1582)
                     /locus_tag="VFM00_11085"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET7498835.1"
                     /translation="MSMIPVRVGGLAIDERTKNPVVLLQEIEGNRVLPIWIGPMEASA
                     IHSELMGKKYQRPLTHDLLVTIIESLRGKISRVVITDLKDSTFFASIYLDAGAGAVAI
                     DARPSDSIAIALRSHAPIFIIDKLFEKSMEPQVGGAPAEKSPEEKAEDLRHFLEELRP
                     EDFGKWNPEDL"
     gene            complement(1608..2912)
                     /locus_tag="VFM00_11090"
     CDS             complement(1608..2912)
                     /locus_tag="VFM00_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET7498836.1"
                     /translation="MSRASSRLRSSLGPRAIALAALVATVAASGCGGGSKARPVAGGS
                     GATSTRVWSPGKADTLGTVVAIVGTRRIRAHEVDSLIATAPPNVQSQLRDPQGYKTLV
                     DRMVTEEALYQAARRSGIQKDPAYRAAVAKAARETLMRQYYQSRMANFPPPTDAQIQA
                     YYEEHSSEFAIPARARVRHIQVATKAKAEALRKRLAAGGLWDALAKANSTDKATSDHG
                     GLVGYVTPSSDFVPGIGKAPDIVKEAFALKEGETSPPLKSEKGWHLIRVDNVDPARTQ
                     PLAEVRQGILGHLSTQAQETLSQTFLDSLKNAAGAVVFEDSVKVASTPARSAQDFFKE
                     AQAASSPQQRIQLYRDVVTRYPDDPVSVQAAFMIGFTYAEDLGQYDEAKAEFRKFLDK
                     YPRHELANSAKWMLENMDKPAPELDDAPEGADSTGAPPDTTR"
     gene            complement(2909..3352)
                     /gene="rplI"
                     /locus_tag="VFM00_11095"
     CDS             complement(2909..3352)
                     /gene="rplI"
                     /locus_tag="VFM00_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET7498837.1"
                     /translation="MEVILLEEMEGLGKRGATVRVADGYARNYLLPRKKAIAAVGNSQ
                     AVFRDRERARVALEQKQRQSAQELRERMMQASLQFSVQVGEEDQLYGSVTAVEIHEAL
                     AAQGFPVERKAVRLEEPIKALGVFEVPVHLFQDIEAPVKVWVVRK"
     gene            complement(3367..4341)
                     /locus_tag="VFM00_11100"
     CDS             complement(3367..4341)
                     /locus_tag="VFM00_11100"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HET7498838.1"
                     /translation="MREAASPAEWIRAAGVLLFGAGIVMAANTDIPVPLPWAYGLLPF
                     AVFWLARHVGLLVGALAATATLGAVYAAGVTEPSRLVFVAVLGTAGLLLSASARRGDR
                     ASTAMTLAAAPILAVVVGYLLLGGMDELSRTFAARLAEVRRLESDLRFSETLGVSRAE
                     FEIALGQMERIYSFLLPSLFALKWISVLSVNVWLTSVLFRERGIPAFRDFSTWRVHPA
                     AAWAFAAALALVVTRLPWATLAGANLAFPLLLAYTVQGLAVARFTAIAFEMNALVQGA
                     ILVLVAFMPILFLAFLAIGFLDSWYDFRRRLVPGPGGSVGRRADKSDD"
     gene            complement(4349..4591)
                     /gene="rpsR"
                     /locus_tag="VFM00_11105"
     CDS             complement(4349..4591)
                     /gene="rpsR"
                     /locus_tag="VFM00_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET7498839.1"
                     /translation="MARDGESRSYRRKFCKLCFDKVDRIDYKDDRRLSRFITDRGKIV
                     PRRISGTCARHQSQITEAVKRARILALLPFTSDQYR"
     gene            complement(4648..5031)
                     /gene="rpsF"
                     /locus_tag="VFM00_11110"
     CDS             complement(4648..5031)
                     /gene="rpsF"
                     /locus_tag="VFM00_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET7498840.1"
                     /translation="MKLYETTIIFDPGLEEARINEELDRVTGPIAQAGGEVVEVQRWG
                     KKKLAYNIKKRRDGTYIHVKHKSPATLIAEMDRRFRLNESVLRHLTVLAPKESPRPAE
                     EAKTEGAPRAEGVPAPAEPAPTAAE"
     gene            complement(5040..5624)
                     /gene="pth"
                     /locus_tag="VFM00_11115"
     CDS             complement(5040..5624)
                     /gene="pth"
                     /locus_tag="VFM00_11115"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET7498841.1"
                     /translation="MKVVLGLGNPGPEYAATRHNAGWMAVDALAKRLGRFQGWTKGPG
                     MSRGEGRAGRHTVLLAKPERYMNRSGDAFRALLAEGWTASEILVACDEVYLPLGSLRI
                     RSRGGTAGHQGLESIALSAGTDAFPRLRIGVGPAPTSDRLKEYVLEPFIEEEKAVLPR
                     VIEAAASAAFDAAAEGLTQAMNRWNRFVLDPVEP"
     gene            complement(5638..6303)
                     /locus_tag="VFM00_11120"
     CDS             complement(5638..6303)
                     /locus_tag="VFM00_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET7498842.1"
                     /translation="MAITSLNGEQRTGVGKGIAKKLRAAGRIPAVYYGRGETPISLVV
                     NAKDVDTLLQGASGANVIVDLQVSGAAAADRKALIREIQRDPVQGNILHLDLQHISLT
                     ERITVEVPIELVGVPTGVKDGGGILEHLLREVEVECLPTDIPTHLEVDVSAMNIGDTL
                     HVSDIKAERVVIITEADRGIATVVPPTVLEEAKPAEEAPAEPELVASKKEGEEAAEGG
                     KEG"
     gene            complement(6359..7333)
                     /locus_tag="VFM00_11125"
     CDS             complement(6359..7333)
                     /locus_tag="VFM00_11125"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET7498843.1"
                     /translation="MSKPLSETHDARHVERGLRLFSGNANRPLAEAICAHLGIDLGDA
                     TVEQFADGETLVKINENIRGSDIFVFQPTFPPASNLMELLIMVDAVRRASARRVTAVI
                     PYYGFQRQDRKDQPRVPITAKLVANLITTAGADRVMTMDLHSAQIQGFFDIPFDHLYA
                     APVLVDYFLRRQIPNVVVVAPDIGSVKMARAYAKRLSASLAIVDKRRPRPDAVEMMNV
                     IGEVEGKNVVIFDDIVSTGSTLVEAAKALKDAGARDIYAAATHGVLCGEAADLIAHSS
                     VKELVVTDSIPHAPGSLPKEIRVLSVAPLLAEAIRRIHDEESLSSLFI"
     gene            complement(7616..7690)
                     /locus_tag="VFM00_11130"
     tRNA            complement(7616..7690)
                     /locus_tag="VFM00_11130"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7656..7658),aa:Gln,seq:ttg)
     gene            complement(7750..8040)
                     /gene="spoVG"
                     /locus_tag="VFM00_11135"
     CDS             complement(7750..8040)
                     /gene="spoVG"
                     /locus_tag="VFM00_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HET7498844.1"
                     /translation="MGLEITEVRIALRDDDKLKAFASMTFNDAFVIRGLKIIEGTNGM
                     FVAMPSRKKPDGTFQDIAHPINNESREKIEARILDEYEKERAKQLIESSQAS"
     gene            complement(8146..9081)
                     /gene="ispE"
                     /locus_tag="VFM00_11140"
     CDS             complement(8146..9081)
                     /gene="ispE"
                     /locus_tag="VFM00_11140"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET7498845.1"
                     /translation="MAAPARGAAPRSAAPAAERLRVRCPAKINLGLWILGRRPDGYHE
                     VDTFLQAISLEDEITIERGTAGLSLTTAGLPIRGPDPNIIERAWALLGETGALPAGAG
                     VRARLVKRIPPGSGLGGGSSDAAGFLAGVDRLLGLNLGSETLAALAGRLGSDVPFFLR
                     GGLARATGRGERVRHLCPITPFWVVLATPATAISTTWAYGQMRKGLTPNGEGARVLAS
                     AITRRDLHGVADAMENAFEGVILPHLPGIAELKRVLVSSGAIGALLSGSGSTVFGVSQ
                     SLSEAQAVLNAVGNRGAEFRIARSIEHGAVVSRAG"
     gene            complement(9083..>9688)
                     /locus_tag="VFM00_11145"
     CDS             complement(9083..>9688)
                     /locus_tag="VFM00_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HET7498846.1"
                     /translation="GSFLLGPRFGMSASAYVALTITYSLALKNVVLLDVVAIAIGFVV
                     RAVAGVELLRDRLGSAEAVELSPWLLVCAFFLALFLAIGKRRHELGVLEGEASRHRAA
                     LGRYTVRLLDQLVAVVTSATVLAYSVYTIAPETVAKFHGRPLFLTIPFVLYGIFRYLY
                     LMYAEEKGGNPSEHLLTDRATLVNVLLWCLVVLLILAWPAA"
BASE COUNT         1480 a         3422 c         3270 g         1516 t
ORIGIN      
        1 acgaaggtct tgccgtagcg ctcgtagcgg cccgagaggg gcacgatcac gccgacccgc
       61 gcgatcccct gcgcggcccg cttgagcgcg accgaatcga gggggcagct ctccagccgg
      121 tccacgatcg agcgaagctc cttgcgctgg ctgccggtcg ccttccccgc gagcccgatc
      181 cgcgcggccc agagctgctc ggcggcgcgg gtcatcggat cgaggcgctg cacgtcgccc
      241 gaggcggcga actcgagcgc ccagccgggc gatgcctcgg gatcgggagc ttcgaggagg
      301 cgcgccttga gctccgcggc gcggcgcggc ggatagtgcc gcgtggaggc tgccggcgcg
      361 acggcgggcg tagcggtggt ggagggcgcc ggagcggccg tggtggccgc gggcgtcgtc
      421 acggggtcgg cggctcgcgc cccgccgccg cgcgatgcgg cgagaagcgc gaccgcgagg
      481 cccgcggcgc cgagagcgag gctcgccctg gtaccgcctc tagcgctcac ggatcgcttt
      541 cgcgtgttcg cgcgccagct cgacgtagac gtccgccgat tcgcgcaggc gggcgagcgc
      601 ctccggagga atcgcgccgc ggatgcgcgc cggcatgccg gcgacgaagg accggggcgg
      661 cacgcgcatc ccctcgagca cgaccgcgcc ggcggcgacc agcgattctt cgccgatctc
      721 gcagtcgtcg agcaggatcg agcccatgcc gatgaggcag cggtcccgca cgacggcgcc
      781 gtggaggatc acgcggtggc ccaccgtcac ctcgtcgccg atggtgaggc cagaccgccc
      841 cgtcgtggcg tgcagcatgc agaggtcctg gatgttcgag cgggcgccga cgcgaatcgg
      901 tccggcgtcg ccgcgcacga cggcgccgta ccagacgctg gactgctcgc ccaggagcac
      961 gtcgccgagg acgacggcgt tgggagcgac gaacgcggag gccggcaccc ggggcgagga
     1021 ccccagcagg gggccgatca cgcgggacgc gcgcgccggg gacgggctac aggtcttcgg
     1081 gattccactt gccgaagtct tcggggcgaa gctcctcgag gaagtggcgg aggtcctccg
     1141 ccttctcctc gggcgacttc tcggccggcg cgcctcccac ctggggctcc atcgactttt
     1201 cgaacagctt gtcgatgatg aagatggggg cgtgggagcg gagggcgatc gcgatcgaat
     1261 cgctggggcg ggcgtcgatg gcgaccgcgc ccgctccggc gtcgaggtag atgctggcga
     1321 agaaggtgga gtccttgagg tcggtgatca cgacgcgcga gatcttccct ctcaggctct
     1381 cgatgatcgt gaccaggaga tcgtgcgtca gcgggcgctg atacttcttg cccatcagct
     1441 cgctgtggat cgcgctggcc tccatcgggc cgatccagat gggcaggacg cgattccctt
     1501 cgatctcctg cagcagcacg acggggttct tcgttcgctc gtcgatggcc aggccaccga
     1561 cgcgtaccgg aatcatgctc atggcgagcc gcgctctcgg cggaggccta ccgtgtggta
     1621 tccggtggcg ctcccgtgct gtccgcgccc tcgggcgcgt cgtcgagctc gggggcgggc
     1681 ttgtccatgt tctccagcat ccacttcgcc gaattcgcca gctcgtggcg cggatacttg
     1741 tcgaggaact tccggaactc cgctttggcc tcgtcgtact gaccgagatc ctccgcgtag
     1801 gtgaagccta tcatgaacgc cgcttgcacg gacaccgggt cgtcggggta gcgcgtcacc
     1861 acgtcgcggt agagctggat gcgctgctgc gggctgctcg ccgcctgcgc ttccttgaag
     1921 aagtcctggg cgctgcgggc cggcgtcgac gcgaccttga cggaatcctc gaagacgacc
     1981 gcgcccgcgg cgttcttgag cgagtcgagg aaggtctgcg agagcgtctc ctgggcctgc
     2041 gtcgagagat gtccgaggat gccctgccgc acctcggcca gcggctgcgt ccgcgccgga
     2101 tccacgttgt ccacgcggat caggtgccac cccttctcgc tcttcagggg cgggctggtc
     2161 tcgccctcct tcaacgcgaa cgcttccttg acgatgtccg gcgccttgcc gatccccggg
     2221 acgaaatcgg agctcggggt gacgtatccg acgaggccgc catggtccga ggtggccttg
     2281 tcggtggagt tcgccttggc cagcgcgtcc cagaggccac ccgccgcgag ccgcttccga
     2341 agcgcctcgg ccttggcctt ggtcgcgacc tggatgtgcc gcacgcgcgc gcgcgccgga
     2401 atcgcgaact cggacgaatg ctcctcgtag taggcctgga tctgggcgtc ggtgggaggc
     2461 gggaagttcg ccatccgcga ctggtagtac tgccgcatca gcgtctcgcg cgcggccttg
     2521 gcaaccgccg cccggtacgc gggatccttc tggatccccg accggcgcgc cgcctgatag
     2581 agcgcctcct cggtgaccat ccggtcgacc agggtcttgt agccctgcgg atcccggagc
     2641 tggctctgca cgttgggggg cgccgtcgcg atcagcgaat cgacctcgtg cgcgcggatc
     2701 cgccgcgtgc cgacgatcgc cacgaccgtg ccgagggtgt ccgccttccc gggcgaccag
     2761 acgcgcgtcg acgtggcgcc cgagccgccc gcgacgggac gggccttgga gccgccgccg
     2821 cagccggacg cggccaccgt ggcgacgagg gccgcgagcg cgatcgcgcg cgggcccagg
     2881 gaagaccgga gccgcgacga agcgcggctc acttccggac cacccacacc ttcaccgggg
     2941 cttcgatgtc ctggaagagg tgcaccggga cttcgaacac gccgagcgcc ttgatcggct
     3001 cttcgaggcg gaccgccttg cgctccacgg ggaacccctg cgccgccagc gcctcgtgga
     3061 tctccaccgc ggtgaccgag ccgtagagct ggtcctcttc gcccacctgg acgctgaact
     3121 ggagcgatgc ctgcatcatc cgctcccgga gctcctgcgc cgactgacgc tgcttctgct
     3181 ccagcgccac gcgggcccgc tcgcggtcgc ggaagaccgc ctgcgagttt cccaccgccg
     3241 cgatggcctt cttgcgcggg agcaggtaat tgcgcgcata gccgtccgcc acgcgcaccg
     3301 tggcgccgcg cttgcccaga ccttccatct cctccagcag aatcacttcc atcgaacgat
     3361 accctcctaa tcgtcggact tgtcggcccg ccgcccgacc gaaccgccgg gcccgggcac
     3421 cagccggcgc cgaaagtcgt accaggaatc caggaacccg atcgcgagaa acgcgaggaa
     3481 cagaatcggc atgaacgcca cgaggacgag gatcgcgccc tgcacgagcg cgttcatctc
     3541 gaaggcgatc gcggtgaagc gcgcgaccgc gagcccctgc accgtgtagg cgagcaggag
     3601 gggaaacgcg agattcgcgc cggccaacgt ggcccaggga aggcgcgtca cgaccagagc
     3661 cagcgccgcg gcgaacgccc aggccgcggc cggatgcacg cgccacgtgg agaaatcgcg
     3721 gaacgcggga atcccgcgct cgcggaagag cacgctcgtg agccagacgt tcaccgacag
     3781 cacgctgatc cacttgagcg cgaagagcga ggggaggagg aacgagtaga tgcgctccat
     3841 ctgcccgagc gcgatctcga actccgcgcg cgagactccg agcgtctccg agaagcggag
     3901 gtccgactcc agacggcgca cctcggcgag ccgcgcggcg aacgtgcgcg agagctcgtc
     3961 catgccgccg aggagcagat agccgaccac gacggccagg atcggggccg cggccagcgt
     4021 catcgccgtg gacgcgcggt cgccccggcg cgccgaggcg ctcaggagaa gacccgccgt
     4081 gcccagcacg gcgacgaaca cgaggcgcga gggctcggtg acccccgccg cgtacaccgc
     4141 gcccagcgtc gcggtcgccg cgagcgctcc gaccaggagc cccacgtgcc gcgccagcca
     4201 gaagacggcg aacgggagca ggccgtacgc ccatggcagc ggcacgggaa tatccgtgtt
     4261 cgcggccatg acgatgcccg ccccgaagag cagcaccccc gccgcgcgga tccactccgc
     4321 gggggaggcg gcctccctca ccttcgaatc agcgatattg gtcactggtg aacggcagaa
     4381 gagccaggat gcgcgcccgc ttcacggcct cggtgatctg ggactggtgc cgggcgcagg
     4441 tgccggaaat ccgccgaggg acgatcttac cgcgatcggt gatgaaccgc gacagacgcc
     4501 ggtcgtcctt gtagtcgatc cggtccacct tgtcgaaaca gagtttgcag aacttgcgtc
     4561 gatacgaccg cgattcgcca tcgcgagcca tgggtctcct cctctagggt tgcctgcggg
     4621 acgccgccct cgggcggacg tcccgcgtca ttccgctgcc gtgggcgcgg gctcggccgg
     4681 cgccggaacg ccttcggccc ggggcgcgcc ttccgtcttg gcctcttcgg ccggacgcgg
     4741 cgattccttg ggtgccagga ccgtcagatg ccggagcacg ctctcgttca ggcggaatct
     4801 ccggtccatc tccgcgatca gggtcgccgg gctcttgtgc ttgacgtgga tgtacgtgcc
     4861 gtcccgccgc ttcttgatgt tgtaggcgag cttcttcttg ccccaccgct ggacctcgac
     4921 gacttcgccc cccgcctgcg cgatcgggcc cgtcacgcgg tccagttcct cgttgatccg
     4981 cgcctcttcg aggcccggat cgaagatgat cgtcgtttcg tacagtttca tgggttccct
     5041 catggctcaa ccggatcgag cacgaaccgg ttccaccggt tcatggcctg cgtgagtcct
     5101 tccgcggccg cgtcgaacgc ggccgaggcc gccgcctcga tcacgcgcgg cagcacggcc
     5161 ttttcttcct ctataaacgg ctccagaacg tattccttga gccgatccga cgtgggggcg
     5221 ggcccgacgc cgatgcgcag cctggggaaa gcgtccgtcc ccgccgacag cgcgatcgac
     5281 tccagcccct ggtgccccgc cgttcccccg cgggagcgga tccgaagcga ccccagcggc
     5341 aggtacacct cgtcgcacgc caccaggatc tccgacgccg tccacccctc cgccaggagc
     5401 gcccggaacg cgtcgccgct ccggttcatg tagcgctcgg gcttggcgag aaggaccgtg
     5461 tgccgcccgg ccctgccctc gccccgggac atccccgggc ccttcgtcca cccctggaag
     5521 cgccccagcc gcttcgccag cgcgtccacc gccatccagc ccgcgttgtg gcgcgtcgcc
     5581 gcgtattccg gcccggggtt tcccagtccc aggacgacct tgatcgtgtg cgcctcgtta
     5641 accttccttg cccccttccg ccgcctcttc gccttccttc ttcgaggcca cgagctccgg
     5701 ctccgccggc gcttcctcgg ccggcttcgc ctcctcgagc accgtcggcg ggacgaccgt
     5761 ggcgatgccg cggtccgctt cggtgatgat cacgacccgc tccgccttga tgtcgctgac
     5821 gtgcagcgtg tcaccgatgt tcatcgcgga cacgtcgacc tcgaggtggg tgggaatgtc
     5881 ggtcggaaga cactcgacct ccacctcgcg caggaggtgc tccaggatgc cgccgccgtc
     5941 cttgacgccg gtgggcactc cgacgagctc gatcgggacc tcgacggtga tccgctcggt
     6001 gagggagatg tgctgcagat ccaggtgcag gatgttcccc tgcaccgggt cgcgctggat
     6061 ctcccggatg agcgctttgc ggtcggcggc cgcggccccc gacacctgga ggtccacgat
     6121 gacgttcgcg ccggatgctc cctgcagcag cgtgtccacg tccttcgcgt tcacgacgag
     6181 ggagatcggc gtctcgcccc gtccgtaata gacggcggga atgcgtccgg cggcgcggag
     6241 cttcttggcg atgcccttgc cgacgccggt ccgctgctcg ccgttcaaac tggtgatggc
     6301 catgatcgat gctcctgggt gattcgtgcg cgcgcccccg ggggcgcgcc ggtttccgtc
     6361 agatgaacag cgagctgagc gactcctcgt cgtggatgcg gcggatcgcc tccgccagaa
     6421 gcggcgcgac cgagagcacc cggatctcct tgggcagcga gccgggggcg tgcgggatgc
     6481 tgtccgtgac gaccagctcc ttcacggagg agtgcgcgat cagatccgcg gcctcgccgc
     6541 acagcacgcc gtgcgtcgcc gccgcgtaga tgtcccgggc gcccgcgtcc ttgagcgcct
     6601 tcgccgcctc gaccagggtg gagcccgtgc tcacgatgtc gtcgaagatc acgacgttct
     6661 ttccctcgac ctcgccgatc acgttcatca tctccaccgc gtccggacgg ggccgccgct
     6721 tgtccacgat cgcgaggctg gccgagaggc gcttggcgta tgcccgggcc atcttgacgc
     6781 tcccgatgtc cggcgccacg acgacgacgt tcgggatctg ccggcgaagg aagtagtcca
     6841 ccagcaccgg cgcggcgtac aggtgatcga acggaatgtc gaagaaaccc tggatctggg
     6901 ccgagtgcag atccatcgtc atcacgcggt cggcccccgc cgtcgtgatg agattcgcga
     6961 ccagcttcgc ggtgatcggg acccgcggct gatctttccg atcctgccgc tggaatccgt
     7021 agtacgggat caccgccgtc acgcgccgcg ccgaggcgcg ccgcaccgcg tccaccatga
     7081 tcagcagctc catgagattg gacgccggcg ggaacgtcgg ctggaagacg aagatgtccg
     7141 agccgcggat gttctcgttg atcttgacga gcgtctcccc gtcggcgaac tgctccaccg
     7201 tcgcgtcgcc gaggtcgatc ccgagatgcg cgcagatcgc ttcggcgagg ggacggttcg
     7261 cgttccccga gaagagacgg agtccccgct ccacgtggcg cgcgtcgtgc gtctcggaga
     7321 gcggcttgga catgggcacg gcgcggcgat cggtggccag cttgaccgtc atcggactcc
     7381 ctcctccgga ccgcaacgaa acgaccccaa tgaaaaagac ccaaagcccg attgacgcca
     7441 tcgtcaatcg ggcccgagac gccgcgcggc gctcgatgga ccggaggcat cctactgccg
     7501 ggcccccggg gccctgtgac cgaggtcaga ttcgaacctt gggtctaccc ggcggagccg
     7561 cttcgccccg ccgctgccaa cttcgggtgt gtgacgtcga tgataccgaa aacggtggtt
     7621 ggggcgggag gattcgaacc tccgaatggg agatccaaag tctcctggct taccacttgc
     7681 cgacgcccca tccgccgcag ggccgccggc gaccggtgct gccggcgccc gccgctcgcc
     7741 tgccctatgt cagctggctt gcgagctttc gatcaactgc ttcgcccgct ccttctcgta
     7801 ctcgtcgagg atgcgggctt cgatcttctc acgcgactcg ttgttgatcg ggtgcgcgat
     7861 gtcctgaaag gttccgtccg gcttcttgcg gctcggcatg gccacgaaca tcccgttcgt
     7921 gccttcgatg atcttgagac cccggatcac gaaggcgtcg ttgaacgtca tgctcgcgaa
     7981 agccttcagc ttgtcatcgt ccctgagtgc gatccgtact tctgtgatct cgagacccat
     8041 ccgtctcacc cacctcgagt tcgggacctc gatccttcga aggccctggg accatccccc
     8101 ccggtcccgg tgatacaccc tgcgccccca acgtggacgg tgcggtcagc cagctcggga
     8161 aaccacggcg ccatgttcga tgctccgagc gatacggaat tcggcgccgc ggttgccgac
     8221 cgcattcaga accgcctgcg cctcggatag cgattgggac acgccgaaaa ccgtcgagcc
     8281 actgccggat aggagcgcgc cgatcgcccc tgaggagacg agtacgcgct tcaattcggc
     8341 gataccgggg agatgtggca ggatcacgcc ctcgaacgcg ttctccattg cgtccgccac
     8401 cccatgaaga tcccgccgtg tgatcgccga cgcgaggact ctagccccct ccccatttgg
     8461 tgtcaacccc tttctcatct gcccgtaggc ccaagtcgtt gaaatcgcgg tagctggagt
     8521 cgccagcacc acccagaacg gcgtgatggg acatagatgt ctcaccctct ccccgcggcc
     8581 cgtagcgcgc gcgaggcctc cgcgcaggaa aaaggggacg tccgaaccca gtcttccggc
     8641 gagcgcggcc agggtctcgg aaccgaggtt cagcccgaga agccggtcga cgcccgcgag
     8701 gaagccggcc gcgtcgctgc tccccccgcc gagccccgag cccggcggga tccgcttcac
     8761 gagacgcgcg cgcacgccgg ctccagcggg aagcgcgccc gtctctccca gtagcgccca
     8821 ggcgcgctcg atgatgttgg ggtcgggtcc gcgaatgggc agcccggccg tcgtgagtga
     8881 aagccccgcg gtgccgcgct cgatcgtgat ctcgtcctcg agcgagatcg cctggaggaa
     8941 cgtgtccacc tcgtgatagc cgtcgggacg ccggcccagg atccagaggc ccaggttgat
     9001 cttcgcgggg catcgcacgc gaagccgttc ggcggccggc gcggcgcttc gcggcgcggc
     9061 gcctcgagcg ggcgcggcca cgtcaggccg ccggccaggc gaggatcagg agcaccacca
     9121 ggcaccagag gagcacgttc acgagggtcg cgcgatcggt gagcaggtgc tccgaggggt
     9181 ttcccccctt ctcctccgcg tacatcagat acaggtagcg aaagatcccg tagagcacga
     9241 acgggatcgt gaggaagagc ggccggccgt ggaacttggc cacggtctcg ggcgcgatgg
     9301 tgtagaccga gtaggcgagc accgtcgcgc tcgtgacgac ggccacgagc tgatcgagga
     9361 ggcgaacggt gtagcggccc agcgcggcgc ggtgccgcga cgcttccccc tccaggacgc
     9421 ccagctcgtg gcgccgcttt ccgatcgcga ggaagagcgc caggaagaac gcgcagacga
     9481 ggagccacgg cgagagctcc accgcctcgg ccgagcccag ccggtcgcgc aggagctcca
     9541 cgccggccac ggcgcgcacg acgaagccga tcgcgatcgc caccacgtcc agcagcacca
     9601 cgttcttcag cgcgagcgag taggtgatgg tgagtgcgac gtacgccgag gcgctcatcc
     9661 cgaatcgcgg cccgagcagg aacgagcc
//
LOCUS       DASSLZ010000295        12710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1366588, whole genome shotgun sequence.
ACCESSION   DASSLZ010000295 DASSLZ010000000
VERSION     DASSLZ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12710
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366588"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFM00_11150"
     CDS             <1..649
                     /locus_tag="VFM00_11150"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7498847.1"
                     /translation="LRYPEALKNDPTIDAEVVLDAVSAVKLLQARPEIDAKRVFVIGH
                     SLGAQLAPEIATRAGSVAGVALLAPPGRRPLAIVAAQMGYLGAPAESIAKVESYFETM
                     TSGLFGGNPILLGAPLSYWADWTSRSDFAPAQARKLGRPVLVLHGERDFQIVDEDIAV
                     WKKRLSGVKDVRFVSMPGLNHLFIEGTGKPGPAEYDTPGHVDPRVVDELARFISR"
     gene            complement(639..1622)
                     /locus_tag="VFM00_11155"
     CDS             complement(639..1622)
                     /locus_tag="VFM00_11155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014368896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7498848.1"
                     /translation="MKKRMLGGRLQVSALGLGCMGMSDFFAQRDDAESIATIHRAIDL
                     GITFFDTADVYGPFTNEVLVGKALQGRRDRVVLATKFGNVRGAEGQWLGVNGKPDYVR
                     QACDASLKRLGTDHIDLYYQHRVDRTVPIEDTVGAMADLVRAGKVRHLGLSEASPATI
                     RRAQAVHPITALQTEYSLWTRDPEDEILPTCRELGIGFVPYSPLGRGFLTGRFQKPED
                     LAPDDARRSHPRFQGENFQKNLDLVRRVEEIAREKRCTPSQLALAWLLAQGKEIVPIP
                     GTKRRAYLEENAAAASIALSEEDLRRIEEAAPQGATSGPRYPEGGMLTING"
     gene            complement(1672..2590)
                     /locus_tag="VFM00_11160"
                     /pseudo
     CDS             complement(1672..2590)
                     /locus_tag="VFM00_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682011.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(2776..3585)
                     /locus_tag="VFM00_11165"
     CDS             complement(2776..3585)
                     /locus_tag="VFM00_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498849.1"
                     /translation="MKRSIVWTAGAAALALCLSAAPTSRAYASRHYHPARAITIVEPA
                     ATVAAGSRVYHVTDDPSYDLSGTSHTLFLVDDGTMCRTPAGRSVAFVGAGSVPTEVIS
                     IPASYRQDWMAVAAGDRPVRCLTPVHGAAVEPSTMSMAPASEESEAEYRAAHPARYTA
                     THRRAYRRNHKKLSFASYKPARTTTYQASEPADYEYAATASYAVDEGVVDHELYQLGN
                     SWYMQDGQVWCRAESWRGPYVQVKKGMVPREVREVAKAREDRDDDDKGIED"
     gene            complement(4197..4913)
                     /locus_tag="VFM00_11170"
     CDS             complement(4197..4913)
                     /locus_tag="VFM00_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7498850.1"
                     /translation="MPNPVAGGRSSAATKQEILAKVPFIDEASPELRNALAENAVVVK
                     LRAGEYFLREGDSCAHFAVVASGRMRVFKLGENGHEITLYHVGPGEACPLNVSCILSE
                     TTVPAMACVEDDVEAIVVPAATFRRWIAEHESLRAFVFRMFAGRLTEVMSLVEEVAFR
                     RMDRRLAGRLAELFESDGSDGSVEITHAELAANLGTAREVVSRLLKEFERLDAIRLQR
                     GKIFVRNSALLRTLAKRGEA"
     gene            complement(4927..7155)
                     /locus_tag="VFM00_11175"
     CDS             complement(4927..7155)
                     /locus_tag="VFM00_11175"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET7498851.1"
                     /translation="MLRRAAVLALVLSIPALPYEAPGARAATAPARRVATARHKAPPP
                     LPRAPKRTVFDTYWGVKVPDDYRYMERASDPGVMKWVRGQNAYTRSWLDGHRERAEIQ
                     KRVAALSRSDSPDFYSGSWAGGRYFFLEEKPPKQQPFLVAMRSLTDRAGETTVVDPNA
                     LDASGGTTIDFYVPSLDGKLAAVSLSKNGTEDGTLHVYETDTGRPRDVAIPRVNGGTA
                     GGSATWNGDGTGLYYTRYPAPGERPKADLPFYQQIWFHKLGTPLSSDTYVLGREFPKI
                     AEAALATSRDGRWILADVSNGDGGEHEYWLMAPGGAWSQVSTFQDGLIAAHVGQDGAL
                     YALSRAGAPNKKILRIPLDHPRLDAARVVVPETQNAIESFAPAASRLYVREMVGGPTQ
                     LRVYDLSGKDLGVVALPEPASIGGAVVTDGDDAMVRVQSYTRPPATYVMAGGSTRLSP
                     TALAQTSPADFSDCEVRREFATADDGAKIPVNIVLRKGTPKDGSAPLLLYGYGSYGLS
                     ETPYFRASIRAWIEQGGVYVDASVRGGGEFGDAWHDAARLGTKKRSMDDFAACARYLV
                     QEGYTRRDRLAIEGGSAGGLLVYGTVVHHPDVMGAAVAEVGYGDVLRTEFSPNGEFNT
                     TEFGTVKDSTLFRQMLAYSPYHHVKAGTAYPSVLALTGVNDPRVESWETYKMVARLQA
                     SGTRRPVLMRVSYDSGHIGSSLSEEDRQNADVFQFLFDQLGVTYRPVKEAERPKSARP
                     SL"
     gene            7324..7473
                     /locus_tag="VFM00_11180"
     CDS             7324..7473
                     /locus_tag="VFM00_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HET7498852.1"
                     /translation="MLRILGVLLLVLWLVGMLAFKTLGAIVHLFLVLAIILFLVGLFR
                     GRRAV"
     gene            complement(7518..8372)
                     /locus_tag="VFM00_11185"
     CDS             complement(7518..8372)
                     /locus_tag="VFM00_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HET7498853.1"
                     /translation="MFRHRAADLQPKMRADSMEPLEPTIATAIVRPPGPTFAQGITRA
                     GLGPPDLELALAQHAAYAVALEAAGVCLVRLPADPAHPDSTFVEDTAVILGREAILTR
                     PGAESRRGEVDSIRAALLPLVASLEAIEPPGTLDGGDVLETDRGFLIGISERTNEAGA
                     RRLVKWLAARGRHAHLVAIGGMPGLLHLKTGISCVAGGLAAAVPALAPAARALGLEVI
                     PVEPEESYAANAIRANGRVLLPSGAPVLSRRLRARGCAVVPLEMSEFEKMDGGLSCLS
                     LRLPAGGG"
     gene            8419..10293
                     /gene="nagB"
                     /locus_tag="VFM00_11190"
     CDS             8419..10293
                     /gene="nagB"
                     /locus_tag="VFM00_11190"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET7498854.1"
                     /translation="MLEEPERIPVVVSASYDEVAVRIARRIGEILRARATAGRSAVLG
                     LATGSTPIGVYRELIRMHREEGLDWSHVKTFNLDEYYPMAPDSYHSYHRFMWENLFAH
                     VNIPRENVHIPRGDIPRDQIEAHCREYEHAIAEAGGIDFQILGIGQTGHIGFNEPGSS
                     VASRTRLVVLDTITRRVAAADFFGAENVPTEAVTMGVATILDAREIALIATGEHKAAI
                     VKRSVEGEVDRSVAATYLQRHPNATFHLDPAAAAELTRVKTPWLLHEVEWTPALAIVA
                     VVWLAQETGKSVLKLDDVDYREHRLGTLLTRYGSSGALNGVVFNALGAKVRGRSRLPR
                     GQRIVVFSPHPDDDVISAGGILWKLRQNENDITIAYQTSGNIAVFDHEVRRYLDFARR
                     LVADFHPGAEDFLGFLDDVETSLARKRPGDVDPEAVLQIKRRIREVEAVSAIETLRFR
                     REETRFLNLPFYETGKVRKDPIGERDVEIVLEVLEERRPEILLVAGDLSDPHGTHRMC
                     LDAVDQALLRYSGPAPEVWLYRGAWQEWSVTEADLLVPMSEEELRLKISAIFKHQSQK
                     DRAPFPGVDEREFWERVEERNKATAAVVDQLGLPEYFAMEALVVRPPAPALIPSAIAP
                     "
     gene            complement(10280..>12710)
                     /locus_tag="VFM00_11195"
     CDS             complement(10280..>12710)
                     /locus_tag="VFM00_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET7498855.1"
                     /translation="HQSERLIGESINPSHRLVRSRARPKLLLAKTFEEAWALFLRYQP
                     YLLGLISDVEFPRGGTPSREAGFDLARMARERIADLPIVLQSSRPEFAAGAARVGAAF
                     LRKYSETLLSDLRAFMVENFAFGDFIFRMENGAEVDRARDMKSLEAKLGTAPAESIAF
                     HAQRNHFSNWLSARTEFALARKLRPRRVDDFDTLEELRRDLIASIAEYRREQSETLVA
                     DFDPDTFDPESNFFARIGNGSLGGKARGLAFVRYLLNYQGIGRRLPGVRIAVPPAVVL
                     ATDCFDRFLARRELRDLALNSRDDDRIIAEFLATPLPRDVVRKLRHFVETVRWPLAVR
                     SSSLLEDSQYQPFTGVYETFMLPNNHPDPKVRLRELVGAIRRVYASTFLRQAKDYLHA
                     TPYRLEEEKMAVAIQKVVGAKHGTRFYPDFSGVARSFNYYASEPLKPEDGVAAVALGM
                     GRGVVGGERCLTFCPRHPQAIVQFSDVEEILKNSQREFWAVELDDAGGDGDPERGLRE
                     KRFGLEAAERDGTLYAMGSTYSAENHAIYDGLARPGVRLVTFAPILKHGVFPLADVIT
                     VLLNVGSHGMNRPAEIEFAGCLSSREDRPHEFGFLQMRPLVVARETEDLDLSDAPPEV
                     LICRSTQVLGNGVVDSIRDAVVVDFHRFDRSASRAAAAEVARFNAELSEAGRPYLLIG
                     VGRWGSTDPWLGIPVTWDQIAGARVIVESGFRDFRVTPSQGSHFFQNLTSFQVGYFTV
                     NADLGEGFLDWDWIGAQPAESKLEFVRHLRFDAPAVVKMNGRRNLGLIFKPAAPAVAN
                     GTGEPGAATAR"
BASE COUNT         1939 a         4381 c         4421 g         1969 t
ORIGIN      
        1 cctgcgctac cccgaggcgc tgaagaacga tcccaccatc gacgccgaag tggtcctgga
       61 tgcggtctcc gcggtgaagc tgctgcaggc gcgtccggag atcgatgcga agcgcgtgtt
      121 cgtgatcggg catagcctgg gcgcgcagct cgcgccggag atcgcgacgc gagcgggatc
      181 cgtcgctggg gtcgcgctgc tcgcgccccc gggacggaga cccctagcga tcgtggcggc
      241 tcaaatgggg tacctgggag cacccgccga atcgatcgcg aaggtggaat cgtatttcga
      301 gacgatgact tccgggttgt tcggaggaaa tccgatcttg ctcggagcgc cgctttctta
      361 ttgggcggat tggacctcac gctctgattt cgcacccgcc caggcccgga agctcggtcg
      421 tcccgtgctc gtgctgcacg gcgagcgcga cttccagatc gtggacgagg atatcgcggt
      481 ctggaagaag cggctctctg gggtgaagga cgtgcggttc gtgtccatgc ccggcttgaa
      541 tcacctcttc atcgaaggga ccggcaagcc cggacccgcg gagtacgaca cgccgggcca
      601 cgtggacccg cgcgtggtcg acgagctcgc ccgcttcatc agccgttgat cgtgagcatt
      661 cccccctcgg ggtagcgcgg ccccgaggtc gctccctgcg gcgcggcctc ctcgatccgc
      721 cgtaggtctt cctcgctcag cgcgatcgag gcggcggcgg cgttctcctc gagataggcg
      781 cggcgcttgg tcccggggat gggaacgatc tccttcccct gggcgagcag ccaggcaagc
      841 gcgagctgcg agggcgtgca gcgcttctcc cgcgcgatct cctccacgcg gcgcaccagg
      901 tcgaggttct tctggaagtt ctccccctgg aaccgcgggt gactccggcg cgcgtcgtcg
      961 ggggccaggt cctccggctt ctggaagcgg ccggtcagga agccgcgccc cagcggactg
     1021 taggggacga agccgatgcc caattcacgg caggtcggga ggatttcgtc ctcgggatcg
     1081 cgagtccaga gcgagtactc ggtctggagg gcggtgatcg ggtgcacggc ctgcgcgcgg
     1141 cggatcgtgg ccggcgacgc ctccgacagt cccaggtggc gcaccttgcc ggcgcgaacc
     1201 agatcggcca tcgctccgac ggtatcctcg atcggcacgg tgcggtccac acggtgctgg
     1261 tagtagaggt cgatgtggtc ggtgcccagc cgcttcagcg acgcgtcgca cgcctggcgc
     1321 acgtagtccg gcttcccgtt cacgcccagc cactgcccct cggcgccccg gacgtttccg
     1381 aacttggtcg ccagcaccac gcggtcgcgc cgcccctgga gcgccttgcc caccaggact
     1441 tcgttcgtaa agggtccgta gacgtcggcg gtgtcgaaaa aggtgatccc gagatcgatc
     1501 gcgcggtgga tcgtggcgat cgactccgcg tcgtcgcgct gcgcgaagaa atcggacatg
     1561 cccatgcagc ccagaccgag ggccgagacc tgaagcctcc cgcccagcat tcgcttcttc
     1621 atggctgtcg ctccgcaatg ccagccgggt ggccggcggg atgagttcgg ctcaggaggc
     1681 cggcagcgcg ggttcgattc ccgtcaccgt cgcggcctcg ggggccacag cgcgcttccg
     1741 gcgcttgagc cgcgcctgga acgcgtcgag ataggtatag atcacggggg tcacgtacag
     1801 ggtcacgagc tgcgagaagg ccagtccgcc gaccaccgca aggcccagcg gccggcgcga
     1861 ctccgctccg gcgccgaatc cgatggcgat cggcagggtg cccatgaggg cggccagcgt
     1921 ggtcatcatg atcggacgga agcggatggg gcaggcctcg acgatcgcct ccttcggagg
     1981 cctcccttcg ttgcgctccg cgtccagcgc gaagtcgatc atcatgatcg cgttcttctt
     2041 caccaaaccc acgagcatga tgatccccac gaacgcgtag cgagccgtcc ttcgacttga
     2101 ggtagaggcc gcgcagggcg ttcacgtcct gctgaaactg cgggaggagc tcgaggatga
     2161 ccccgtactg gtcgctcgac gtgaaaatgg tcgagacctg ccgcgagccg aaggaatcgt
     2221 agagcgtctc ctcgatctgg cgcgccgaga tgccgagcgc ggaggcgcgg tcacgatcga
     2281 tctgcaccga gacctgggga ttggcgatct gcaggtcgct ggtcacgtcc tgcagatcgg
     2341 ggagatcgcg catgcggtcg aggagctggt cggccgaggc gtagagcccc gcgatgtcgc
     2401 cgctctgcag cgtgtactgg tactggctct tggacatgcg ccctcccacg ttgatcgtgg
     2461 gtgggttctg catgtagacg cggatccccg gcacctgccc caccttgggc tgcaaccgcc
     2521 ggatcacctc gtcggccgag gcgccccgct cgttccgggg cttcaaccgg atgatcatcc
     2581 gcccctggtc cctcgggcac atcaggggat actaccggac ccctcaccct gttccaaggg
     2641 tggggggaac gcccgcccca gggccgcgga acggcgcccg agcaagcgaa cggggctccc
     2701 gaaatcggag ccccgttcgt tgtagcgggg tagcggggta gcggggaagc ggagaagcgg
     2761 agaagcgagt acgtgctagt cctcgatccc cttgtcgtcg tcgtcgcgat cctcgcgggc
     2821 cttggcgacc tcacgcacct cgcgcggaac catccccttc ttcacctgca cgtagggacc
     2881 gcgccacgat tccgcgcggc accatacctg gccgtcctgc atgtaccagg agttgcccag
     2941 ctggtagagc tcgtggtcga ccacgccctc atcgacggcg tacgaggccg tcgccgcgta
     3001 ctcgtaatcg gccggctcgc tcgcctgata ggtcgtggtc cgcgccggct tgtacgacgc
     3061 gaaggagagc ttcttgtggt ttctccggta ggcacggcga tgcgtcgccg tgtagcgcgc
     3121 gggatgagcc gcgcgatact ccgcctccga ctcctcgctc gcgggcgcca tgctcatcgt
     3181 ggacggctcg acggccgcgc cgtgcaccgg cgtgaggcac cggaccggcc ggtcgcccgc
     3241 ggcgaccgcc atccaatcct gccggtacga cgccggaatc gagatcacct cggtcggcac
     3301 cgagcccgcg cccacgaaag cgacggaccg tccggccgga gtccggcaca tcgtgccgtc
     3361 gtccaccagg aacagcgtgt ggctcgtgcc ggagaggtcg tagctggggt cgtcggtgac
     3421 gtgatagacg cgcgagcccg cggccaccgt cgcggcgggc tcgacgatgg tgatggcgcg
     3481 ggccgggtga tagtgccggc tcgcgtaggc gcgcgatgtg ggcgccgcgg acaggcacaa
     3541 ggcgagcgcc gccgcgcccg cggtccagac aatcgatcgt ttcatagggc ctccgtggca
     3601 gcggccctgg gtcagggtgg ggccgcgagg tccgctcatc gtcgcatgtc gtgtgccgga
     3661 atgcggccgc gggccggagt tgtgagccgg agaccgatat tcgtaggcca ggacggtccc
     3721 ggaggggcgc gggcgaaccg cggagagcgg ccgcaagggc gtcggggccc tagaactgcc
     3781 gacccataac ctattgtaga aatgcggctt gtattgccgt tttcatgttt cttgcgaacc
     3841 gtaccgggac aggcgccccc ttgccaaggg tgcgctggcc tggggcccga ctagggagcc
     3901 gtcttgggtc cggttcgggt cgcggggcgt ccccctccgt tccacagggg gcaccggggc
     3961 gaagagattt caaggcggag ggagctttcg accacggggg ccggatccga aaaaatacgc
     4021 ggcttcgtga cttctgtcac ggagcggccg ccgagcggcc gtcattctgt cctcagccgg
     4081 cggtgctcgg catcaccctc cggctcgacc ctcacccata gccagccagc ttcctgaaga
     4141 gggacgggcc actccccacg ggtcggcgtt gcgccgccgg tcctctgttc tccgccctac
     4201 gcttcgcccc gcttggccag ggtgcgaagg agcgcgctgt tcctcacgaa gatcttcccc
     4261 cgctggagcc ggatcgcgtc cagcctctcg aattccttga gcagccggct caccacctcg
     4321 cgcgccgtgc cgagattcgc ggcgagctcg gcgtgggtga tctccaccga cccgtccgac
     4381 ccatcgctct cgaacagctc ggccagacgc cccgccagcc gccggtccat gcggcggaac
     4441 gcgacctctt cgaccagcga catcacctcg gtgagccgtc ccgcgaacat gcggaagacg
     4501 aaggcgcgga gcgattcgtg ctcggcgatc cagcgccgga acgtggcggc cggaacgacg
     4561 atcgcctcga cgtcgtcctc gacgcacgcc atggccggca ctgtcgtctc ggagaggatg
     4621 caggagacgt tgagcgggca ggcctccccg gggccgacgt gatagagcgt gatctcgtga
     4681 ccgttctcgc ccagcttgaa gacccgcatc ctgccgctcg cgaccacggc gaaatgggcg
     4741 cacgaatccc cttcgcgcag gaagtattcg cccgcgcgca gcttcacgac gaccgcattc
     4801 tcggccaggg cgttccggag ctccggcgag gcctcgtcga tgaacggaac cttggccagg
     4861 atttcctgct tcgtggcggc cgacgagcgg ccgccggcga ccggattcgg catggcgttc
     4921 ctcccgctag agactgggtc tggcgctctt cggccgctcg gcttccttca cgggacggta
     4981 ggtcaccccg agctggtcga agaggaactg gaacacatcg gcgttctgcc ggtcttcctc
     5041 ggacagcgag ctgccgatgt gaccggagtc gtagctcacg cgcatcagca ccggacgccg
     5101 cgtcccggag gcctggagcc gcgcgaccat cttgtaggtc tcccacgact cgacgcgcgg
     5161 atcgttcacc ccggtgagcg cgagcaccga gggataggcg gtgcccgcct tcacgtggtg
     5221 atacggcgag tacgccagca tctggcggaa gagggtcgaa tccttgaccg tcccgaactc
     5281 ggtcgtgttg aactcgccgt tgggcgagaa ctcggtgcgc aggacgtcgc catatccgac
     5341 ctcggccacg gccgccccca tcacgtcggg atggtgaacc accgtgccgt agacgaggag
     5401 cccgcccgcg ctgccgccct cgatcgcgag acggtcgcgg cgggtgtagc cctcctggac
     5461 gaggtagcgc gcgcacgcag cgaaatcgtc catcgagcgc ttcttcgttc cgagacgcgc
     5521 cgcgtcgtgc caggcgtcgc cgaactctcc gcctccgcgg acgctcgcgt cgacgtagac
     5581 cccaccctgt tcgatccagg ctcggatcga cgcgcggaag tacggagtct cgctcaggcc
     5641 gtagctccca tatccgtaca gtaagagcgg ggcgcttccg tccttgggcg ttcccttccg
     5701 gaggacgatg ttgacgggga tcttcgcgcc gtcgtcggcc gtcgcgaact cgcgacggac
     5761 ctcgcagtcg ctgaaatccg ccggagaggt ctgcgcgagc gccgtcggcg aaagccgggt
     5821 cgatccgccg gccatcacat aggtcgccgg ggggcgggta tagctctgca cgcgcaccat
     5881 ggcgtcgtcg ccgtcggtca ccacggcgcc gccgatcgac gcgggctcgg gcaacgcgac
     5941 cacgccgagg tccttcccgc tcagatcgta gacccggagc tgcgtcggac cgccgaccat
     6001 ctcgcgcacg tacaggcggg acgccgcggg cgcgaacgac tcgatcgcgt tctgggtctc
     6061 ggggacgacc acgcgcgcgg cgtcgagtcg gggatggtcc agggggatgc gcaggatctt
     6121 cttgttcggc gcccccgcgc gcgagagcgc gtagagcgcg ccgtcctggc cgacatgcgc
     6181 ggcgatgagc ccgtcctgga acgtcgagac ctggctccac gcgccgcccg gcgccatcag
     6241 ccagtactcg tgctcgccgc cgtcgccgtt gctgacgtcg gccaggatcc agcgtccatc
     6301 ccggctcgtc gccagggccg cctcggcgat cttcggaaac tcgcgcccaa ggacgtaggt
     6361 gtcggacgag agcggcgtcc cgagcttgtg gaaccagatc tgctgataga acggcaggtc
     6421 ggccttgggc cgctcgccgg gagcgggata gcgcgtgtag tagagcccgg tcccgtctcc
     6481 gttccacgtg gcgcttcctc cggccgtccc cccgttcacg cgcgggatcg cgacgtcccg
     6541 cgggcgtccg gtatcggttt cgtagacgtg gagggtgccg tcctcggtgc cgttcttgga
     6601 gagggagacg gcggcgagct tgccatccag cgagggaacg tagaagtcga tcgtcgttcc
     6661 gcccgaggcg tccagcgcgt tcggatcgac caccgtcgtt tccccggcgc ggtcggtaag
     6721 ggatcgcatc gcgaccagga acggctgctg cttcggcggc ttctcttcga ggaagaagta
     6781 gcggcccccg gcccagctcc cgctgtagaa gtccggagag tcggagcggc tgagcgccgc
     6841 cacccgcttc tggatctccg cgcgctcccg gtgcccgtcc agccacgagc gcgtgtaggc
     6901 gttctggccc cggacccact tcatcacgcc gggatcggac gcgcgctcca tgtagcggta
     6961 gtcgtccgga accttcaccc cccagtaggt gtcgaagacg gtccgcttgg gagcccgcgg
     7021 cagcggcgga ggcgccttgt ggcgcgcggt cgcgacgcgc cgcgcgggag ccgtggcggc
     7081 ccgcgcgccc ggcgcctcgt acggaagcgc aggaatcgag agaacgagag cgagtacggc
     7141 ggctcggcga agcatgggtc ggtctcctgg acgatggggg ggcgtggccc cggggggccg
     7201 ttccgatgat agcacccccc cgccgcgcgc acggggcccg gttcctgctg agggagtctg
     7261 ctgacatgcg gtccccggcc gggtcctacc acccggcggc ccgcgcgaat cgggaggaac
     7321 accatgcttc gaatcctggg agtgctgctc ctcgtcctct ggctggtcgg aatgctcgcg
     7381 ttcaagacgc tgggcgccat cgtgcacctg ttcctggtgc tcgcgatcat cctcttcctg
     7441 gtgggcctgt tccggggccg cagggccgtc tgacagggcg ggggggcgcg cccccgccga
     7501 tgcggcgtga gccgcgctca gccgcctccg gcaggcagcc gaagcgagag gcacgagagc
     7561 ccgccgtcca ttttttcaaa ctcggacatt tcgagcggaa cgaccgcgca tcccctcgcg
     7621 cgcaggcgcc gcgagagcac cggcgcgccc gatggaagca gcacgcggcc gttcgcccgg
     7681 atggcgttcg cggcgtagga ctcctcgggc tcgacgggga tcacctcgag gccgagcgcg
     7741 cgggcggcgg gcgcgagcgc tgggaccgcc gcggcaagcc cgccggccac gcaggagatc
     7801 cccgtcttca agtgcagaag tcccggcatt ccgccgatcg ccacgagatg cgcgtgacgg
     7861 ccgcgagccg ccagccattt cacaaggcgg cgagcccccg cttcgttcgt ccgctccgag
     7921 atcccgatga ggaagccccg gtcggtctcg agcacgtccc ctccgtccag cgtgccgggc
     7981 ggctcgatcg cctcgagcga ggcgacgagc ggaaggagtg ctgcgcgaat cgagtccact
     8041 tccccgcgcc ggctctcggc gccggggcgg gtgaggatcg cctcccgccc caggatcacg
     8101 gcggtgtcct ccacgaacgt ggaatcggga tgagccggat cggccggcag gcggaccagg
     8161 cagacgcccg cggcctccag cgccaccgcg taggccgcat gctgcgcgag cgccagctcg
     8221 aggtcggggg gcccgagccc cgcccgggtg ataccctggg cgaacgtcgg tcccggcggc
     8281 cggacgatgg ccgtggcgat ggtgggttcc aggggttcca tggaatcggc ccgcatctta
     8341 ggctggagat cggccgcgcg gtgtcgaaac attctacggc cccgccgctt caccgaacgc
     8401 aggagaccgc gcacgagcat gctcgaagaa cccgaacgga tcccggtcgt cgtctcggcc
     8461 tcctacgacg aggtcgcggt ccgcatcgcc cgccggatcg gcgagatcct gcgcgcgcgc
     8521 gccaccgccg gccggagcgc cgtcctcggc ttggccaccg gttcgacccc catcggcgtc
     8581 taccgcgagc tgatccggat gcatcgcgag gagggactcg actggtccca cgtgaagacc
     8641 ttcaacctcg acgagtacta ccccatggcg ccggacagct atcacagcta ccaccggttc
     8701 atgtgggaga acctcttcgc gcacgtgaac atcccgcggg agaacgtgca cattccgcgc
     8761 ggcgacatcc cccgcgacca gatcgaggcg cactgccgcg aatacgagca cgccatcgcc
     8821 gaggccggcg gcatcgactt ccagatcctc ggtatcggcc agaccggaca catcggcttc
     8881 aacgagccgg gatccagcgt ggcgtcccgc acgcgcctgg tcgtgctcga cacgatcacg
     8941 cggcgcgtgg ccgcggccga cttcttcggc gccgagaacg tgcccaccga ggcggtcacg
     9001 atgggggtgg cgaccatcct cgacgcgcgc gagatcgccc tgatcgccac cggcgagcac
     9061 aaggcggcga tcgtgaagcg ctccgtcgag ggcgaggtgg accggtcggt cgcggccacc
     9121 tatctccagc ggcaccccaa cgccaccttc cacctggatc cggccgccgc cgccgagctc
     9181 acgcgcgtga agaccccctg gctcctccac gaggtggagt ggacgccggc cctcgcgatc
     9241 gtcgcggtcg tgtggctggc gcaggagacc ggcaagtcgg tgctcaagct ggacgacgtg
     9301 gactaccgcg agcaccggct cggcaccctg ctcacccgct acggctcctc cggtgctttg
     9361 aacggcgtgg tgttcaacgc gctcggcgcc aaggtgcgag ggcggagccg cctcccgcgc
     9421 ggccagcgga tcgtggtctt ctcgccccat cccgacgacg acgtgatctc ggcgggtgga
     9481 atcctttgga agctccggca gaatgagaac gacatcacga tcgcctatca gacctcgggg
     9541 aacatcgcgg tcttcgacca tgaggtgcgg cgctacctgg atttcgcgcg ccggctggtg
     9601 gccgacttcc atccgggcgc ggaggacttc ctcggcttcc tggacgacgt cgagacgtcg
     9661 ctggcgcgaa agcgccccgg ggacgtggac cccgaggcgg tgctccagat caagcggcgg
     9721 atccgcgagg tggaagcggt ctcggccatc gagacgctgc gcttccgccg cgaggagacc
     9781 cgctttctga acctcccctt ctacgagacc ggcaaggtcc ggaaggatcc gatcggagag
     9841 cgcgacgtcg agatcgtgct cgaggtgctg gaggagcggc gtccggagat cctcctcgtc
     9901 gccggagacc tctccgatcc gcacgggacg caccggatgt gcctggacgc cgtggaccag
     9961 gcccttctgc gctactccgg cccggcaccc gaggtgtggc tctaccgagg cgcgtggcag
    10021 gagtggtcgg tgaccgaggc ggatctgctc gtgccgatgt cggaggagga actgcggctc
    10081 aagatcagcg cgatcttcaa gcaccagagc cagaaggatc gcgccccctt ccccggggtg
    10141 gacgaacggg agttctggga gcgggtcgag gagcgtaaca aggcgaccgc cgccgtcgtg
    10201 gaccagttgg ggctgcccga atacttcgcg atggaggcgc tggtcgtgcg ccctccggcc
    10261 ccggcgctca ttccctcggc tatcgcgccg tagcggctcc cggctccccg gtcccgttgg
    10321 cgaccgccgg ggccgcgggc ttgaagatca ggcccaggtt cctccggccg ttcatcttca
    10381 cgaccgcggg cgcgtcgaag cgcaggtgcc ggacgaactc cagcttcgac tccgccggct
    10441 gcgccccgat ccagtcccag tcgaggaatc cctcccccag gtccgcgttg accgtgaagt
    10501 accccacctg gaacgacgtc aggttctgga agaagtggct cccctgcgag ggagtcacgc
    10561 ggaagtcgcg gaaccccgac tcgacgatca cgcgcgcgcc cgcgatctgg tcccaggtga
    10621 cgggaatgcc gagccacgga tcggtggatc cccagcgtcc cacgccgatc aggagatagg
    10681 gccgccccgc ctcggagagc tccgcgttga agcgagccac ctcggcggcg gcggcgcggc
    10741 tggcggagcg gtcgaagcgg tggaagtcga cgacgaccgc gtcgcggatc gaatccacga
    10801 ccccgttgcc gaggacctgc gtgctgcggc agatcagcac ctcggggggc gcgtcggaca
    10861 ggtcgaggtc ctcggtctcc cgcgccacca ctaaaggccg catctggagg aagccgaact
    10921 cgtgcggccg gtcctcgcgg gaggagaggc agcccgcgaa ctcgatctcc gccggccggt
    10981 tcatgccgtg gctcccgacg ttcagcagca cggtgatgac gtcggccagc gggaaaacgc
    11041 catgcttcaa gatgggcgcg aaggtcacga ggcggacgcc gggccgggcc agcccgtcgt
    11101 agatcgcgtg gttctcggcc gaataggtcg agcccatggc gtagagcgtg ccgtcccgtt
    11161 cggccgcctc cagcccgaag cgcttctcgc gcaggccccg ctcggggtcg ccgtcgcctc
    11221 ccgcgtcgtc cagctccacc gcccagaact cgcgctggga gttcttcaag atctcctcga
    11281 cgtccgagaa ctgcacgatg gcctgcggat gccgcgggca gaaggtgagg cagcgctccc
    11341 cgcccacgac gccgcgcccc atccccagcg cgacggccgc cacgccgtcc tccggcttca
    11401 ggggctcgct cgcgtagtag ttgaaggagc gcgccacgcc cgagaagtcg gggtagaagc
    11461 gggtgccgtg cttcgccccc acgaccttct ggatggccac cgccatcttc tcctcctcca
    11521 ggcgatacgg ggtcgcgtgg aggtagtcct tcgcctggcg gaggaacgtg gaggcgtaga
    11581 cgcgccggat cgcgccgacc agctcgcgca gccgcacctt ggggtcgggg tggttgttcg
    11641 ggagcatgaa ggtctcatac acgcccgtga acggctggta ctgggagtcc tcgagcaggc
    11701 tggagctccg caccgccagc ggccagcgca ccgtctcgac gaagtggcgg agcttccgca
    11761 cgacgtcgcg cgggagcggc gtcgcgagga actcggcgat gatccggtcg tcgtcccggc
    11821 tgttcagggc cagatcccgg agctcgcggc gggccaggaa gcggtcgaag cagtcggtgg
    11881 ccagcacgac cgcgggcgga acggcaatgc gcacgcccgg gagcctccgg ccgatcccct
    11941 ggtagttgag caggtagcgc acgaacgcca gcccgcgcgc cttgcctccc agggagccgt
    12001 ttccgatccg cgcgaagaaa ttgctctcgg gatcgaacgt gtcggggtcg aagtcggcga
    12061 ccagggtctc gctctgctcg cggcggtact ccgcgatcga ggcgatcagg tcgcggcgca
    12121 gctcctcgag cgtgtcgaaa tcgtccacgc ggcgcgggcg cagctttcgg gccagcgcga
    12181 actcggtccg cgccgagagc cagttcgaga agtggttccg ctgggcgtgg aacgcgatgc
    12241 tctcggcggg cgcggtgccg agcttggcct ccaggctctt catgtcgcgc gcccggtcca
    12301 cctcggcgcc gttctccatc cggaagatga agtcgccgaa cgcgaagttc tcgaccatga
    12361 aggcgcgcag atcggagagg agggtctcgg agtacttgcg caggaaggcg gcgcccaccc
    12421 gcgcggcgcc tgccgcgaac tcggggcggc tcgactgcag cacgatcggc aggtcggcga
    12481 tccgctcgcg cgccatccgc gccaggtcga agcccgcctc gcggctgggc gtccccccgc
    12541 gcgggaactc cacgtccgag atcaatccga ggaggtaggg ctggtagcgc aggaagagcg
    12601 cccaggcctc ctcgaacgtc ttcgcgagga gaagcttcgg gcgggcgcgg gagcggacca
    12661 gccggtgcga ggggttgatc gactccccga tcaggcgctc cgactggtgg
//
LOCUS       DASSLZ010000296         7633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1855725, whole genome shotgun sequence.
ACCESSION   DASSLZ010000296 DASSLZ010000000
VERSION     DASSLZ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855725"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFM00_11200"
     CDS             complement(<1..185)
                     /locus_tag="VFM00_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498856.1"
                     /translation="MALAFAGLLAPSRPYVAASLVAGQLLAALNFLFLERLVKLFTQA
                     SLSKAPADAAGARAGVL"
     gene            complement(252..482)
                     /locus_tag="VFM00_11205"
     CDS             complement(252..482)
                     /locus_tag="VFM00_11205"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET7498857.1"
                     /translation="MTEKDPKKVTAWRQVGLLTTIPFILALAPIVGYLLGQYLDNRFH
                     TRPWLSVVLLALGFVAGVRETVNIIKLSQRED"
     gene            1201..1785
                     /locus_tag="VFM00_11210"
     CDS             1201..1785
                     /locus_tag="VFM00_11210"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7498858.1"
                     /translation="MAESKSAASLWMDEGLESDRVRAAQRGDREAYRQLIRHYERPVY
                     RLAYALTRNHDEATALAVETFARGLTEITRLPEGKRFFPWLLRFARNLSVTRARRRAD
                     AGAKALGAITPESPAAANSETSPVELEQRLLDALGELRPDEQMALALKVGERLSYADI
                     ATLLDHSAGVTLSRLSSARALLLTRTREGIGDLS"
     gene            1782..2774
                     /locus_tag="VFM00_11215"
     CDS             1782..2774
                     /locus_tag="VFM00_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7498859.1"
                     /translation="MIHLTLNQISAYIDAELPEASVELVRLHLSSCLECTERFGHIEE
                     QEGALARLLVNDPGDPFFERFAERVLGPEPVEKAKPVKKPEPVKEAGPVPKAEPVKKP
                     EPPLLVAAAEAPRDPTEPPRPRAAPAPAPKEAPRQRPAPKAAASRPKASGPVTNDGPR
                     KAGAARILLTVAALLIVLSAAAVIAVRPRIPQLSFAPTERQVPDPGAQDARPPRVPSA
                     PSDAVPDPSAGGSDLLERAAARSSEAERAGTAEAHDAAAAAWEEALPQLEKNADELAA
                     GRREIASARYAAWSKDPTPERRDAAMSAVRAYLLCSPAGADRDRAWTWLAELKR"
     gene            2907..5237
                     /locus_tag="VFM00_11220"
     CDS             2907..5237
                     /locus_tag="VFM00_11220"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET7498860.1"
                     /translation="MDEGILAEFLAESFENLAQVEHDLVALERDPEDRKRIGGIFRAV
                     HTIKGTCGFFGFKKLEALAHAGENLLSRLRCGEIAFTPSLADTLLRLVDALHAILSAI
                     ETTRQEGDQEFAPLIEELVHLERAPSGAAAPPDSPSTAEAETGAPPRGGLLDPLIAEG
                     RLDPDAVALAAQQQRLGDPRRIGEILVDNGALKPQDVLDALLARGEAPALAHGESTIR
                     VDVELLDLLMNLVGELVLARNQLLQKVASSDLADLPGTTQRLNLITTELQEGIMKTRM
                     QPIANLCNKLPRLVRDLSIACGKEVRLELEGIDTELDRTIIDAIRDPLTHLIRNAIDH
                     GIETSEARLARGKAAEGRILVRAYHEGGKVHLEVTDDGDGLPIARIREKATRAGLVPA
                     ERIPRMSDRDWAQLIFQPGFSTAERVTNVSGRGVGMDVVKTNVERIGGAIEVDSQPGR
                     GATVRIRIPLTLAIVPALIVAAAGERYAIPQVNLLELIRVEGDLASISITTAVDASLL
                     RYRGGLLPLVDLRDALHVPADDAFPTPAGARPIAVLQADGATFGLVVDAVETSQEIVV
                     KSLGDPLRQIPVFAGATILGDGKVALILDVPGLARHCGITVRGHAAMEEVVDETPAPV
                     VPTQTLILCRAGGYPVAIPQSDIARLEEFPRGTIERLGERRVVQYRGGLLPLVPLAGL
                     LGIGPDAALETAAEAPAGGASLQTIRVVVVEREGHAIGLAVEEIVEILEEPVVPEVSS
                     RAGVRGSAVIRGHATDLLDLEALLASSGQPAMAAGA"
     gene            5253..5687
                     /locus_tag="VFM00_11225"
     CDS             5253..5687
                     /locus_tag="VFM00_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7498861.1"
                     /translation="MEGERVLCTFRVAELFLGLDIASVQEVLRGQEVTRLPLAAAPVR
                     GIINLRGRIVPAVDLRRCLELAPAPESLEPATIVVRGPEAAVSLLVDDVGDVMTVSEE
                     TFARTPETVRASARDLLRGVFPLEDRLLLELDLGRVLRAAYA"
     gene            5746..7485
                     /locus_tag="VFM00_11230"
     CDS             5746..7485
                     /locus_tag="VFM00_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET7498862.1"
                     /translation="MVLTVRRQLVGSALLALVGFLVFGVVAYVTVSELKINGKRYHRI
                     TQGKDLVADVVPPQEYLVESYLAVHQMMGETDRRRLDDLIQQGQELRANFEARHQHWE
                     KTLPPGPLRDALLKDAYEPGAEFLRIKDDEFVPAVRRGDTEGARQIADTQLKPLYEQH
                     RKAVLEVVQLARQATEREEKDALALTQQRTALVPLVGGLLALATVGIGLFVSRRLVSA
                     IEQTRQVAERTADGDLTSRVGYSSSDELGQLATATNRMVESLRSILSHIGDRAVTLAT
                     ASEELSSVSTQMISSAEETSQQASMVSSGSEQISRNVESVSVSVEQMIASIQEISKNT
                     SEAARVATVAAGEADLTNTAVNKLGESSAEIGKVVQVINTIAQQTNLLALNATIEAAR
                     AGEAGRGFAVVANEVKELANETATATKDISRKIEAIQADTSEAIGAIGKISDTIQQIK
                     DISNSIATAIEEQLATSGEIGRNLTEAARGSGEISAGIVKVAEIARDTAGSSENTQKA
                     AEELARLASELRALTQRFRYSEEPVAEDAAAHVAAAAPATVLAMAAAKGRARPAGGAE
                     SVAARPPDVRRAG"
BASE COUNT         1192 a         2667 c         2757 g         1017 t
ORIGIN      
        1 cagaggacgc cggcgcgcgc gccggcggcg tccgcgggag ccttcgagag ggacgcctgt
       61 gtgaagagtt tcacaagccg ttcgagaaag aggaagttga gcgccgccag aagctgcccc
      121 gcgacgagcg acgccgcgac gtagggccgc gagggtgcga ggagtcccgc gaaggccagg
      181 gccatgatcc aggacgcccg gagcgaccgg gagaggagcg tcccttcctg cgtcttcgcg
      241 aagaccagcg gctagtcctc cctctgggag agcttgatga tgttgaccgt ctcgcgcacg
      301 cccgccacga aaccgagcgc gagcagaacc acgctcagcc acgggcgcgt gtgaaaccga
      361 ttgtcgagat attgcccgag taagtaaccg acgatgggcg ccagcgcgag gatgaacgga
      421 atcgtcgtca ggagcccgac ctgccgccag gccgtgacct tctttggatc cttctcggtc
      481 actgcttggc ctccgcgaga cgatcgccgc taagccctgc ggcggaccgc gtctgcagcg
      541 acatcgcgac ggcgacttcg agcccgacag gatagtgggt gagggtacgg tggtcaacgg
      601 aaaatggggg ccgcgggatg ttgcgcggcc ccgcgcgggg gcgacgtccg tcgccagaag
      661 aaaaaggggg cgcgcgagcc cggtcgccgg gcgctcgatg gcgccgcgcg gcttccgttc
      721 gcgcctcccc cgaaatttga ggcgggacgc ctcttcgcct cctcggcttc accgcaccgc
      781 ggccccgact ccgcagtgct ctcccccttg agaggacgcc ccgccagttc ttccttcctc
      841 tatatgcccg atccggcttc gcgccggtga tctcccccat cgcaccctcc gtgccgcagg
      901 ctcgcgttcc ggaaccctca atggaagcga cctgattgca gtaacttatg gggcggggcc
      961 agaaaccagg cggacctgcg tgcctttccc ctgcctcccg ccgttgactg acagccgtcc
     1021 cctcgggcgg ccggaccctc gaaaggcctt gagacgcccc cggttgcgtc cgcgtcaacg
     1081 cgggaccgcg ccgtgaaccg gcgttgccgc ggctccagat gggcctcgct cctgccgata
     1141 tcgagcgcgg gaaagtcgcg gctccaaagc cgccggatcg aaccagcgtt ggaggggacc
     1201 gtggcggaat cgaagagcgc ggcgtcgctc tggatggacg aggggctgga atcggaccgg
     1261 gtgcgcgcgg cgcagcgcgg cgatcgcgag gcgtaccggc agctgatccg ccactacgag
     1321 cgcccggtct atcggctggc gtacgcgctc acgcgcaacc acgacgaggc gacggcactc
     1381 gcggtcgaga ccttcgctcg cgggctgacg gagattaccc gactgcccga ggggaagcgc
     1441 ttctttccgt ggctgctccg cttcgcccgg aacctctcgg tgacccgcgc gcgccggcgc
     1501 gccgacgccg gggcgaaggc tctcggcgcg atcacgccgg agagtccggc cgcggcgaat
     1561 tccgagacgt ccccggtcga gctggagcag cggctgctgg atgcactggg cgagctgcgc
     1621 cccgacgagc agatggcgct ggcgctcaag gtcggagagc gcctctccta cgccgacatc
     1681 gccacgctcc tcgaccactc cgcgggggtt accctctcgc ggctctcctc ggcgcgcgcc
     1741 ctcctcctca cgcgcacgcg cgaggggatc ggcgatcttt cgtgatccac ctcacgctga
     1801 accagatctc ggcctacatc gacgcggagc ttcccgaagc gtcggtcgag ctggtgcggc
     1861 tccatctctc ctcctgcctc gagtgcaccg agcgcttcgg acatatcgag gagcaggagg
     1921 gtgcgctggc gcgcctcctg gtcaacgacc ccggggatcc cttcttcgag cggttcgcgg
     1981 agcgggtgct cggaccggag ccggtggaga aggccaagcc ggtgaagaag ccggagccgg
     2041 tgaaagaggc cgggcctgtg ccaaaggccg agcccgtgaa gaagcccgag cccccgcttc
     2101 tggtcgccgc cgcagaagcg ccgcgtgatc cgacggagcc gccccggccc agggccgctc
     2161 cagcgccggc gccgaaggaa gcgccccggc agcggcccgc gcccaaggcg gccgcctcgc
     2221 gcccgaaagc ctcaggcccc gtgaccaacg acgggccgcg aaaagcggga gccgcgcgaa
     2281 tcctcctcac ggtcgcggca ctcctgatcg ttctgtcggc cgcggcggtc atcgcggtcc
     2341 ggccgcggat tccacagctc tcgttcgcgc caaccgagcg ccaggtcccc gaccccggtg
     2401 ctcaggacgc ccggccgccg cgggtgccat ccgctccctc cgacgccgtg cccgatccgt
     2461 cagccggcgg ctccgacctc ctcgaacgcg cggccgcccg aagctccgag gcggaacgcg
     2521 ccggaacggc cgaggcccac gacgctgccg ccgcggcatg ggaagaggct ctgccccagc
     2581 tggagaagaa cgccgacgag ctcgcggccg ggaggcgcga gatcgcgtcc gcccgctacg
     2641 ccgcctggag caaggaccct actccggagc ggcgcgatgc ggccatgagc gcggtgcgcg
     2701 cctatctcct ctgctcgccc gcgggggccg accgcgaccg cgcctggacc tggctggccg
     2761 agctcaaacg ctagctgccg tccccagggc ccggtaaggc gcgtataact ccgcactttt
     2821 cctcaaggtt gccccacccc cggccgatgc atcgatacgt ggcggagcct gcccggcctc
     2881 gcccgacagg gaggatcatg ggagcgatgg acgagggaat cctcgcggag ttcctggccg
     2941 agagcttcga gaatctcgct caggtcgaac acgatctggt cgcgctcgag cgggatcccg
     3001 aggaccggaa gcggatcggc ggcatcttcc gcgcggtgca cacgatcaaa ggcacctgcg
     3061 gcttcttcgg cttcaagaag ctcgaggcat tggcgcacgc cggggagaat ctgctgagcc
     3121 ggctccgctg cggcgagatc gccttcaccc cgagccttgc cgacacgctg ctccggctgg
     3181 tggacgcgct gcacgcgatc ctgtcggcga tcgagacgac gcggcaggaa ggcgaccagg
     3241 agttcgcgcc gctcatcgaa gagctggtgc acctggagcg ggcgccctcc ggggccgcgg
     3301 cgccgcccga ttccccgtcg accgccgagg ccgagaccgg tgctccgccg cggggcggcc
     3361 ttctcgatcc cctcatcgcc gaggggcgcc tcgaccctga cgcggtggcc ctcgcggcgc
     3421 agcagcagcg cctgggggac ccgcgccgga tcggcgagat cctcgtggac aacggcgccc
     3481 tgaaaccgca ggacgtcctg gacgcgctgc tcgcgcgcgg cgaggcgccg gcgctggcgc
     3541 acggcgagag cacgatccgc gtggacgtgg agctgctcga cctcctgatg aacctggtcg
     3601 gcgagctggt cctcgcccgg aatcagctcc tccagaaggt cgcctcgtcg gatctcgcgg
     3661 accttcccgg cacgacccag cgcctcaacc tgatcacgac cgagctgcag gaggggatca
     3721 tgaagacccg catgcagccg atcgcgaacc tctgcaacaa gctcccccgg ctcgtgcgcg
     3781 atctctcgat cgcgtgcggc aaggaagtgc ggctggagct ggaagggatc gacaccgagc
     3841 tggaccgcac gatcatcgac gccatccgcg acccgctgac ccacctgatc cggaacgcca
     3901 tcgaccacgg gatcgagacg agcgaggccc ggctggcccg cggcaaggcc gccgaaggcc
     3961 ggatcctcgt gcgcgcctac cacgaaggcg gcaaggtgca cctggaggtc acggacgacg
     4021 gcgacggcct gccgatcgcg cgcatccgcg agaaagcgac ccgcgcgggg ctggttccgg
     4081 ccgagcggat cccgcgcatg agcgaccgcg actgggcgca gctcatcttc cagcccgggt
     4141 tctcgacggc ggagcgcgtc acgaacgtgt cgggccgcgg cgtcgggatg gacgtcgtca
     4201 agaccaacgt cgagcggatc ggcggcgcga tcgaggtgga ttcgcagccc gggcgcggcg
     4261 ccacggtccg gatccggatc ccgctcacgc tcgcgatcgt ccccgcgctg atcgtggccg
     4321 cggccggcga gcgctacgcg atcccccagg tgaacctcct cgagctgatc cgcgtggagg
     4381 gggacctggc ctcgatctcg atcacgaccg cggtggacgc gtcgctgctc cgctaccgcg
     4441 gcggcctcct tcctctggtg gacctgcgcg acgcgctcca cgttccggcg gacgacgcgt
     4501 tccccacgcc ggcgggcgca cggccgatcg ccgtgctgca ggccgacggg gccacgtttg
     4561 gcctcgtggt ggacgccgtc gagacctcgc aggagatcgt cgtgaagtcg ctgggcgatc
     4621 cgctgcggca gatcccggtc ttcgccggcg ccacgatcct gggcgacggc aaggtcgcgc
     4681 tgatcctgga cgtgcccggt ctggcccggc actgcggcat caccgtgcgg gggcatgccg
     4741 cgatggagga agtcgtcgac gaaacacccg ctcccgtcgt gccgacccag acgctcatcc
     4801 tctgccgcgc cggcggctac ccggtggcca tcccgcagtc ggacatcgcg cgcctcgagg
     4861 aattcccgcg cggcacgatc gagcggctgg gcgagcggcg cgtggtgcag taccggggcg
     4921 ggctcctccc gctggtcccg ctcgcggggc tgctcgggat cggccccgac gccgcgctcg
     4981 agaccgccgc cgaggccccg gcgggcggcg cctcgctgca gacgatccgc gtggtcgtcg
     5041 tggagcggga gggacacgcg atcggcctcg cggtcgagga gatcgtcgag atcctggaag
     5101 agcccgtcgt gcccgaggtc tcatcgcgcg cgggcgtgcg cggctccgcc gtgatccgcg
     5161 ggcacgcgac cgacctcctg gacctcgagg cgctgctcgc gtcgagcggc cagcccgcca
     5221 tggcggcggg agcctgagcg gaggagcgag ccatggaagg agagcgggtg ctctgcacgt
     5281 tccgcgtggc cgagctcttc ctgggcctcg acatcgcgtc ggtccaggag gtgctgcgcg
     5341 gccaggaggt gacgcgcctt ccgctcgcgg cggcgccggt gcgcggcatc atcaacctgc
     5401 gcgggcggat cgtgcccgcc gtggatctgc ggcgctgcct ggagctggcg ccggcgcccg
     5461 agagcctcga gcccgcgacg atcgtcgtgc gggggccgga ggccgcggtc agcctgctcg
     5521 tggatgacgt cggtgacgtg atgacggtgt cggaggagac gttcgcgcgg acgcccgaga
     5581 cggtgcgggc gagcgcgcgc gatctcctgc gcggggtgtt cccgctggaa gaccggctcc
     5641 tcttggagct ggatttgggt cgcgtgctcc gggcggcgta cgcctagggc tcgaagcgaa
     5701 gggacccggc tcatgggccg ggcgccaggg aaggagcgaa cgacgatggt cctcacggtt
     5761 cggcgccagc tggtcgggag cgcgctcttg gctctcgtcg gcttcctcgt gttcggggtg
     5821 gtcgcctacg tcacggtgag cgagctcaag atcaacggga agcgctacca ccggatcacc
     5881 cagggcaagg atctcgtagc ggacgtggtc ccgccgcagg agtacctggt cgagtcctat
     5941 ctcgccgtgc accagatgat gggagagacc gaccgccggc gtctggacga cctgatccag
     6001 caggggcagg agctgcgggc caacttcgag gcgcgccacc agcactggga gaagacgctt
     6061 cccccgggcc cgctccggga cgcgctcctc aaggacgcct atgagccggg cgcggaattc
     6121 ctccggatca aggacgacga gttcgtcccc gccgtgcgcc gcggcgacac cgagggcgcg
     6181 cgtcagatcg ccgacacgca gctgaagccg ctctacgagc agcaccggaa agcggtgctc
     6241 gaggtggtac agctggcccg ccaggcgacc gagcgggagg agaaggacgc cctcgccctg
     6301 acgcagcagc gcacggccct cgtgccgctc gtgggcgggc tgctcgccct ggcgacggtg
     6361 ggcatcggcc tcttcgtgtc ccgccgcctg gtgagcgcga tcgagcagac gcgccaggtg
     6421 gccgagcgca cggccgacgg cgacctcacc tcacgggttg gatattcctc gagcgacgag
     6481 ctgggccagc tcgcgaccgc cacgaaccgc atggtcgagt cgctccggtc gatcctctcc
     6541 cacatcggcg accgcgcggt gacgctggcc acggcgtccg aggagctctc ctcggtgtcg
     6601 acgcagatga tctcgagcgc cgaggagacg tcgcaacagg cgagcatggt ctcctcggga
     6661 tcggagcaga tcagccgcaa cgtggagagc gtctcggtct ccgtggagca gatgatcgcg
     6721 agcatccagg agatctccaa gaatacgagc gaggccgcgc gcgtcgccac cgtggccgcg
     6781 ggcgaagcgg acctcacgaa caccgccgtg aacaagctgg gcgaatcgag cgccgagatc
     6841 ggcaaggtcg tgcaggtgat caacacgatc gcccagcaga cgaacctgct cgcgctgaac
     6901 gccacgatcg aggcggcacg ggcgggcgag gcgggccgcg gcttcgccgt cgtcgcgaac
     6961 gaggtgaagg agctggcgaa cgagaccgcg acggccacca aggacatcag ccggaagatc
     7021 gaggcgatcc aggcggacac gtcggaggcg atcggcgcga tcggcaagat cagcgacacg
     7081 atccagcaga tcaaggacat ctcgaattcg atcgcgaccg cgatcgagga gcagctggcc
     7141 acctcgggcg agatcgggcg caacctgacc gaggcggcgc gcgggagcgg cgagatcagc
     7201 gccggcatcg tgaaggtcgc ggagatcgcg cgcgacaccg cgggcagctc cgagaacacg
     7261 cagaaggcgg cggaggagct ggcgcggctc gcgtcggagc tgcgggccct gacgcagcgc
     7321 ttccgctact cggaggagcc ggtcgccgag gacgcggcgg cgcacgtggc ggcggccgcg
     7381 cccgccacgg tgctggccat ggcggcggcg aagggccgcg cacggcccgc gggcggagcg
     7441 gagagcgtcg cggcgcggcc gcccgacgtg cgccgggcgg gttgagcgga agcgacacga
     7501 cacggggacg aacggcggca tcaggacgag gaggtcggag atgaaggctc tggtggtgga
     7561 tgactcgcgg tccatgcggg ccatcgtcgg aaagcagctt cgcgagctgg ggttcgcagt
     7621 ggaggaggcc ggg
//
LOCUS       DASSLZ010000297        14421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_492015, whole genome shotgun sequence.
ACCESSION   DASSLZ010000297 DASSLZ010000000
VERSION     DASSLZ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492015"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1035)
                     /locus_tag="VFM00_11235"
     CDS             complement(229..1035)
                     /locus_tag="VFM00_11235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7498863.1"
                     /translation="MRIPGFASIVVTGCIALAGPSALAQTASSGDVFTYKLEKTARFE
                     TGCFAMCDCAIVTHPIQGTFTLQHTGFDPLYDYYAVTDVRWVVSDATTYATIHGSGTY
                     KRGGEFALTHQLTLDLTVDGEAPQHFDSGMIVGGSEWPRIVIDVSMHQNSACVDTLIH
                     VDATDPIATGISSTGGLQAPWVKVAPNPFRGATEFSLAVPRASEVKVVVYDASGRAVR
                     HLRSGWMASGVHPLVWDGRRDSGSSCASGVYFVSARVGTEHLTSRIVKVD"
     gene            complement(1137..1985)
                     /locus_tag="VFM00_11240"
     CDS             complement(1137..1985)
                     /locus_tag="VFM00_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498864.1"
                     /translation="MLGRKALFLAASCLLLTGCFSRRGGEVGPIPKPVVPGTIVFASN
                     RDEGHGEIYSMQDDGTHVTRLTFNSVQDAAPLLSPDGTRITFRRELNPSNVFVMNVDG
                     TSQIGLAPGKRAEWSHDGTKLALVADSIAVMNADGTGRQWLHVGGDYVTWSPDGTKLA
                     YVSNGLNGQATNDIYTIFANGTGAHRVTTDGVAKNSLAWSPDGTKFVYSAAGSVYVMN
                     ADGSGLSSPAAGRDARWSPDGSRIIFITDTYDANEEIYTVRLDGTDPKNLSQNPANES
                     DPDWGP"
     gene            complement(2152..3831)
                     /locus_tag="VFM00_11245"
     CDS             complement(2152..3831)
                     /locus_tag="VFM00_11245"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HET7498865.1"
                     /translation="MIGLLSRRRASARAALRRTRHIARVLGANGLESIAEATGLRRFL
                     PRFGKDGKNGEVRSQAERLRVAFGELGVTFIKLGQMLSTRADLLPPEVIAELSKLQDS
                     APPVPFEEIRRILMEDLPGPKLGSFAWIEQRPLASASIGQVHEALLGDGTPVVIKVRR
                     PGVVEQVDQDLQILRQAVDWVQVHTPIGHDYELRPLMAEFAATLRDELDYVREGQNAT
                     KMRIAFGNDRRVWIPRVYDELTTTRVLTMERVGGVKFTDAGRLDQLGISRRALAETAV
                     RLFLRQVFELGFFHADPHPGNFFVQPSGTLAVVDFGMVGRLSEPTQRHLMAAMLAGVR
                     EDAEALAEELYALGVAGARADRVSFERDLARLVNRYGSSSIRDLSAATVTDELFSIAF
                     RHKLQLPSELAQLLRVINMAEGLGLSLDPDFRYLEFATPVIKKLARRRYAPAALARRA
                     GLAAVEAAELGADFPRRAARLLGRFERGEIAIKVDHRGLESATREFQKMTNRLVLAML
                     LAASIVALGLAAGIRMVPALEPVVDWLFRFALLFSLAFGVSVLWGMWRSGR"
     gene            4110..5498
                     /locus_tag="VFM00_11250"
     CDS             4110..5498
                     /locus_tag="VFM00_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET7498866.1"
                     /translation="MAASLLYTALDRLAQAALNAHFRRVRVAHRERFPESGAALLVAN
                     HPSAWADAVVLLGAFGRKLHFLVQGSQFRPWPRGMLLRLFGALPVYARDAHPRAAARN
                     AETFRRCEALFDRGEVVAVFPEGISETDRRLMPLRTGAARLAIAYAGRPGARPLPLSA
                     VGLHYSDRVAFRSDVSVNVGQPIDVYDLVESWDPEVAAVRLTDRIGAEIRMLALGAVR
                     PRQAAVLSALEPIAERMAEGLEIDGPSEVLGRALEDLEREDPETFRRLERRALAQERT
                     RRALRVSDRALAMRRSRRSPARAALSATATALGALPAAVGALIHALPAALTDLATRRI
                     ATTPAQVAFVRIAAGAIFFIAWYVVLGWAVLFGMGVGPEMTAGVLAVCGLLGACALAY
                     TPRAREQLERWRVGWIAFRHLSLVCRAHREEEWLERRVAALLEYALEVADVDPAYTPV
                     LELGARPSPLQR"
     gene            5434..6102
                     /locus_tag="VFM00_11255"
     CDS             5434..6102
                     /locus_tag="VFM00_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7498867.1"
                     /translation="MSILHTRRFSSWARGHLLCSVDERPDRFAITFDDGPNPNATDAV
                     LDVLARHDARATFFMLGGHVRRRPELARRVVAAGHEAAVHGDTHWPMPLLLPGAMRGQ
                     VRRCAASIEAATGTSPRFYRPPFGFMTPGQARFIRGLGFESVLGDVYPEDPHRPGAGR
                     IATRVLRRLSGGSILILHDGSPFGAADRSQTVEALGAILGEMGRRGLRGVTVAELVGD
                     RKIF"
     gene            6158..6634
                     /locus_tag="VFM00_11260"
     CDS             6158..6634
                     /locus_tag="VFM00_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498868.1"
                     /translation="MSAATRVLAVSALVVGLCPALAFACEDEASAKAEASAKTVKVEI
                     CKASTKCTVKIKTSRAEKSVTVVADDTKCSVSAKAMNTQGAHHAWEPGHLIEPDSMKL
                     GPGDDLNAPNAEQWVRISSKSGSVQIHRGPWEKKTEGRTWMNVYGPYRVFLQSQLP"
     gene            complement(6636..7088)
                     /locus_tag="VFM00_11265"
     CDS             complement(6636..7088)
                     /locus_tag="VFM00_11265"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HET7498869.1"
                     /translation="MCRPAVHAARAQCGPRWGASQPWSDFSHHHYPGFGHSPGLGVRR
                     PLRFLAWKLQLDDAQMKQVAAILDQLKIERAQAEVDDRRAVSLLAEASEGDAFDRAKA
                     DEAAALRTKSADRVQRKVVESIERLHALLQPEQRARLAYLLRTGTLVM"
     gene            complement(7229..7942)
                     /locus_tag="VFM00_11270"
     CDS             complement(7229..7942)
                     /locus_tag="VFM00_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498870.1"
                     /translation="MRVLRRVLLALVVAAGVGALGYAVYVGAAWTRFGRVKPQAPPAR
                     AVVSMDRFLPQYDVSERHETKVKAPAPVTYAAARDVDLQRSPLVRAIFESRKILLRSR
                     GAELARRPFLDQVRGMGWGLLAEVPGRAMVFGAVTRPWESTVKFHPLPPAQFTAFHRP
                     GWAKIVWTLEVDPAGEERSLFRTQTRVATTDATARTRFRKYWATMSPGILLIRQQVLG
                     LVRGDAERRARDARQKPAG"
     gene            complement(7939..8793)
                     /locus_tag="VFM00_11275"
     CDS             complement(7939..8793)
                     /locus_tag="VFM00_11275"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET7498871.1"
                     /translation="MMMLARNLRRTYLRFQEHEGARNAASIAFFVLFSAVPSAALLIL
                     SVGWTIHDPRDQAHLLGHMLELLPVGSPQNRAFLLQSVESIRKASGGISVLGVLGLAW
                     SSLGMISATRWGLNRAWGVSGRRGFVRVRVRDFLTGAGIWGLLVVSAASTAGLHVLLG
                     ERQIPAGGMPSGPDLVWATIQWSMPALLSFAGFFFVYWFVPNVTHRMRDVLPAAMVAT
                     FLFEISKYLFGLYVAIIASRSSLFGALGGILGFMLWVYVCSSILLVGAEFASVYRHRR
                     AEKLAGAA"
     gene            8974..9720
                     /locus_tag="VFM00_11280"
     CDS             8974..9720
                     /locus_tag="VFM00_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7498872.1"
                     /translation="MRRVRIILADDYPMDRAGMVSILRSQPDFEIVGEASSVREAGAM
                     CREMNPSVLILAMRLQATEGRTPIASVHAVSPETPVLAIAERAEGHCLVLNPPGSKRT
                     DTAQLPVTCTQGTDCLQLAVAEGATGTIRRSATPDELFRAVRTVASGNAWYEAGTATA
                     IMRHALAGNGSGGSAPAPLSGRELEVAELIAAGRSNKEIGSILNISAPTVKKHVGRIL
                     EKLGLQDRLQVGLYVARNPLVLQPLDTKRR"
     gene            9843..10460
                     /locus_tag="VFM00_11285"
     CDS             9843..10460
                     /locus_tag="VFM00_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET7498873.1"
                     /translation="MNRLGTTLAGAVCAGVLALGFWGVASAEETAAAAAPAAHQYVGA
                     KKCKMCHNAEKGGAQFTKWTESKHAKALETLGSEEAKKIAAEKKIADPATSAECLSCH
                     QTGYGEAADHLAASYVAEGVTCESCHGAGADYIKMKTMAGIRDKSLKAEDYGLVMPTK
                     ETCEKCHNDKSPSFKSFDFAEFSKKIEHKIPEGYKRGGATEEATK"
     gene            10488..12728
                     /locus_tag="VFM00_11290"
     CDS             10488..12728
                     /locus_tag="VFM00_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET7498874.1"
                     /translation="MKRARRPRQRPWAPRCICFPVSVARAAALSWIAGLAVVVALLAF
                     VPAGAAAPASVRIPDSVCIECHTGANLDSAVVRGRKVKLDIDRAVLQHSAHAGVACTQ
                     CHVGFNPEEQPHRARAVPVDCRRCHMNADSAHVFHAKTTFAKAAGAVGSCQGCHGDHD
                     VPKAGAATTPAGARSLDLACARCHREEVAHFENSAHGRAAARGERAAPTCLSCHRLPV
                     TAARVHDQRDARLKLAQEQLCLHCHLDDPAVRSRVGPQTGFIRAYETSIHGQELRRGN
                     GKAATCIDCHGSHEMARGFEPSAKTEKFHIPDTCGRCHDKIAATYRTSVHGQGMAKGN
                     PDTPVCTDCHGEHTIKKADAPESPVAPENVSAEVCSPCHSSVRLATKYGIKSDRFQTF
                     SDSFHGLAIRGGQVSAANCASCHGAHDILPSSDPRSRISPARLAVTCGKCHSGATAAF
                     ARGKVHLDVTRTKEPLLYWIGLIYTILIVGVVGGMFLHNAVDFFRKAQHKLRVRRGLA
                     AAHEPESRALYVRMTGSERAQHATLLTSFTVLVLTGFMLRYPESWPVEQLRHIWHNLF
                     EARSVIHRVAGVAMVAASLFHLYYLGFTKRGREFFRDMLPRPSDLRDVIGALRYYAGL
                     TRQRPKFGRFSYIEKSEYWALVWGTVVMAGTGMILWFQDPFIALLTKLGWDAARSVHF
                     YEAILATLAILVWHIYFVIFNPDVYPMNLAWLKGTITEEEMEDEHPLELEKIRREREA
                     DKEGRS"
     gene            12725..13216
                     /locus_tag="VFM00_11295"
     CDS             12725..13216
                     /locus_tag="VFM00_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET7498875.1"
                     /translation="MSEKDPGQEKDPGQEKDPGQEKDPERRGALVWLVRGFLALWAPA
                     AAGMAASFLKAPSPENRPGERMLRGGPMSSIAVGDARLVRHGTDPVYVVRVSEEQVVA
                     VSAICTHLRCVVKWNRDSGTFRCPCHDGAFDRGGNVLSGPPKKPLQQFPAEVRADEIV
                     IHT"
     gene            13229..14371
                     /locus_tag="VFM00_11300"
     CDS             13229..14371
                     /locus_tag="VFM00_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET7498876.1"
                     /translation="MERNRGGLLGWLEARVNLTEIVSFLTVFGLLPVELDSRKPLKEA
                     LREAMAQPLPSYARWPQVIGVLSFILFVILAVTGTMLAFYYQPTADVAYPSVTLIARD
                     ISFGNLTHQVHHWAAVLFLILLAARVIRFFFARLYHQGREVVWVVAVLTFVAAAIADL
                     TGRLLPWDARGYWTTVRAREVTDALPILGPFTSFLVGGSGLDSLVLTRYYVLHVVVFP
                     LLLIGLFYLHFSSVRRVGLSRNAPTGSGAAPTPVGPRALHVVTYDLILLIILMIGALL
                     TFAVLMPYPFDVVADPFTTPPNARPPWYLLAPHALLEAFPAWVPRPIRGILPEAMLLA
                     VILVPMIDRGTVTDERRRKLFRNIGYSVLVVWVLLTLIGWMLEVRR"
BASE COUNT         2254 a         4953 c         4998 g         2216 t
ORIGIN      
        1 ctgattcgaa gccggacccc gaacggcttt cttcctatgc gcgcggagat ctcgctcgcc
       61 gcgtcatcca agattggcga gcgctgccct ggaggcgaag cgtgagagat tcagtctacg
      121 cggagcgaag gaggtggctc atcgacggta agccgcccta tgggaccttc agggaggaca
      181 tggagcgctc ccgggaagag ctagagcctt agggctcgtc gctcgagatc agtccacctt
      241 cacgatgcga ctcgtgaggt gctccgttcc tacccgagcg ctgacgaagt agacgcccga
      301 ggcgcaggac gaccctgagt cgcgccggcc gtcccatacg aggggatgca cgccggaggc
      361 catccatccg gaacgaagat gccgcacggc gcggccgctg gcgtcgtaga ccacgacctt
      421 gacctcgctg gccctgggca cggccaggct gaactcggtg gcgccccgga agggattggg
      481 ggcgaccttg acccacggcg cctgcaatcc gccggtggac gagatcccgg tcgcgatggg
      541 atcggtggcg tctacgtgga tcagcgtgtc gacgcacgcg ctgttctgat gcatcgagac
      601 gtcgatcacg atccgtggcc actcgcttcc gcccacgatc atcccgctgt cgaagtgctg
      661 cggcgcctcg ccgtccacgg tgaggtccag cgtgagctgg tgcgtgagcg cgaattcgcc
      721 gccccgcttg taggtgccgg agccgtggat ggtcgcgtac gtggtggcgt cggagacgac
      781 ccagcgcacg tcggtgacgg cgtagtagtc gtacagagga tcgaagccgg tgtgttgcag
      841 ggtgaaggtg ccctggatgg gatgcgtgac gatggcgcaa tcgcacatgg cgaagcagcc
      901 cgtttcgaag cgggccgttt tctcgagctt gtaggtgaag acgtcgccgg atgaggcggt
      961 ctgggcgagg gcgctcgggc cggcgagcgc gatacagccg gtgaccacga tggatgcgaa
     1021 cccgggaatt ctcatggctt cctcccctgt tgcgcggtcg aggaccacgc tacacccggg
     1081 ggaagcttgg ctcaaccacc agaatcacgg cccttcgcgg gcccgaacca agcgcctcaa
     1141 ggaccccagt cgggatcgga ttcgttggcc ggattctggc tcaggttctt gggatcggtc
     1201 ccgtccaggc ggaccgtgta gatctcctcg ttggcgtcgt aggtatcggt gatgaagatg
     1261 atcctcgagc cgtcggggga ccaccgcgcg tcgcgcccgg ccgcgggcga cgacaggccg
     1321 ctgccgtccg cgttcatgac gtacacgctg ccggccgcgc tatagacgaa cttggtcccg
     1381 tccggagacc aggcgaggga gttcttggcc acgccgtcgg tggtcacgcg gtgcgcgccc
     1441 gttccgttcg cgaagatggt gtagatgtcg ttggttgcct ggccgttcaa cccgttcgac
     1501 acgtaggcga gcttggtgcc gtcgggactc caggtgacgt agtcgcctcc cacgtggagc
     1561 cactgccggc ccgttccgtc ggcgttcatg acggcgatgc tgtcggccac cagcgcgagc
     1621 ttggtaccgt cgtgcgacca ctcggcgcgt ttccctggcg cgagcccgat ctgcgacgtg
     1681 ccgtccacgt tcatcacgaa cacgttcgag ggattgagct cgcgccggaa ggtgatccgg
     1741 gtgccgtccg gcgagagaag gggggccgca tcctggacgc tgttgaaggt gaggcgcgtg
     1801 acgtgcgtgc cgtcgtcctg catcgagtag atctcgccgt gcccctcatc ccggttgctc
     1861 gcgaacacga tggtgccggg caccacgggc ttgggaatgg ggccgacctc gcccccgcgg
     1921 cgcgagaaac agccggtcag gagcaggcag ctggcagcca ggaacagagc ctttcttccg
     1981 agcacgttca cccccaaccc gatgacctag cggcggcaat gggactgcgg agaccctcat
     2041 cctgtatcgg cagttttggc cgtgaggaac agtgatttcg cggtcggcgt ctcggttgga
     2101 agcgccgcgt ttacagcggc ttatggggcg ctcttcaagc gttctcgggg gctacctgcc
     2161 ggaccgccac atcccccaga gaacggacac cccgaatgcg agcgagaaga ggagcgcgaa
     2221 ccggaacagc cagtccacga ccggctcgag cgcggggacc atacggatcc cggccgccag
     2281 gcccagcgcc acgatcgatg ccgcgagcag catggcgagc accagccggt tggtcatctt
     2341 ctggaactcg cgcgtcgcgc tctccagtcc gcgatggtcc accttgatcg cgatctcgcc
     2401 gcgctcgaag cggccgagca gccgggccgc gcggcgtggg aagtcggcac ccagctcggc
     2461 ggcctcgacg gccgccagac cggcgcggcg cgccagcgcg gcgggggcgt agcgccgccg
     2521 cgccagcttc ttgatgaccg gcgtcgcgaa ctccaggtag cggaagtcgg gatccagcga
     2581 cagccccagt ccctccgcca tgttgatcac gcgcaacagc tgcgccagct cgcttggaag
     2641 ctgcagcttg tggcggaacg cgatcgagaa gagctcgtcg gtgacggtcg cggcgctcag
     2701 gtcgcgaatg gaagaggagc cgtagcggtt gaccagcctg gccagatccc gctcgaacga
     2761 gacgcgatcg gcccgggcgc cggccacccc cagcgcgtac agctcctccg ccagcgcttc
     2821 ggcgtcctcg cggacgcccg cgagcatcgc ggccatgaga tgccgctgcg tcggctcgct
     2881 gagccgtccg accatgccga aatccaccac ggccagcgtg ccgcttggct gcacgaagaa
     2941 gttgccggga tggggatcgg cgtggaagaa gcccagctcg aacacctggc gcaggaagag
     3001 ccgcaccgcg gtctcggcca gcgccctccg cgagataccg agctggtcca gccgcccggc
     3061 gtcggtgaac ttgacaccgc ccacgcgctc catcgtgagc acgcgggtcg tcgtcagctc
     3121 gtcgtagacg cgcgggatcc agacccggcg gtcgttgccg aaggcgatcc gcatcttggt
     3181 cgcgttctgc ccctcgcgca cgtagtccag ctcgtcgcga agcgtggcgg cgaactccgc
     3241 catcagcggg cgcagctcgt agtcgtgccc gatcggggta tggacctgca cccagtcgac
     3301 ggcctggcgc aggatctgga gatcctggtc cacctgctcg accacgcccg gccggcgcac
     3361 cttgatcacg accggcgtcc cgtcgccgag cagcgcctcg tgcacctgac cgatcgacgc
     3421 cgaagccagc ggccgctgct cgatccaggc gaaggagccg agcttgggcc cgggcaggtc
     3481 ttccatgagg atgcggcgaa tctcctcgaa gggcaccggc ggcgccgaat cttgaagctt
     3541 cgacaactcg gcgatcacct ccggagggag caggtcggcg cgcgtgctca gcatctggcc
     3601 gagcttgatg aacgtgaccc ccaattcgcc gaaggcgacg cgcagccgct cggcctgcga
     3661 tcgcacctcg ccgttcttgc cgtccttgcc gaagcgggga aggaagcgac ggagccccgt
     3721 agcctcggcg atcgactcga gaccgttcgc gccgagaacg cgcgcgatgt ggcgcgtgcg
     3781 ccgcagcgcg gcgcgcgccg aagcccgccg tcgagacaga aggccgatca tgacagggca
     3841 ctcctccagt gggggacgcc aacatcgtta cgggcacgtt ctgtgccact ccactggcag
     3901 ggcttcgacc gcctcgaagc ccgtcaagag ggtgtgaagt cgtgatgaat ggcctgggta
     3961 agcagttcgg tccgggagcg aacgggagcg cgccaggggg gagcgcgttc caggcctgcc
     4021 acgatggcag ccgcgctgac gcgatggcag acacgtgccg tccgcgtcgc cgctcgctcg
     4081 ccgggtccgc tgtggccagg gcggccggcg tggcggcgag tcttctctac accgcgctcg
     4141 accgcctggc ccaggccgct ctgaacgcgc atttcaggcg cgtgcgcgtg gcccatcgcg
     4201 agcgatttcc cgagagtggt gccgcgctcc tggtcgccaa tcacccttcc gcctgggcgg
     4261 acgccgtcgt gctgcttggc gcgttcggca ggaagctcca cttcctggtc cagggtagcc
     4321 agttccggcc ctggccgcga ggaatgctgc tgcggctctt cggcgcgctc ccggtctacg
     4381 cgcgcgacgc ccatccccgc gcggcggccc ggaacgccga gacgttccgc cgctgcgaag
     4441 ccctcttcga ccgcggcgaa gtcgtggcgg tgtttccgga ggggatcagc gagaccgacc
     4501 ggcggctgat gccgcttcgc accggcgccg cgcggctcgc gatcgcctac gcggggcggc
     4561 cgggcgcgcg cccgctcccc ctctcggcgg tcgggctcca ctattccgat cgcgtggcgt
     4621 tccggagcga cgtgtcggtg aacgtggggc agccgatcga cgtctacgac ctggtggagt
     4681 cctgggatcc cgaggtggcc gcggtccggc tcacggaccg gatcggtgcg gagatccgaa
     4741 tgctggccct gggggcggtg cgtccccggc aggccgcggt cctctccgcg ctcgagccga
     4801 tcgcggagcg gatggcggag gggctggaga tcgacggtcc ctccgaagtg ctcggccgcg
     4861 cgctcgaaga cctggagcgc gaggacccgg agacgttccg ccggttggag cgccgggctc
     4921 tggcgcagga gcggacccgg cgggcgctcc gcgtctccga tcgcgcgctc gcgatgcggc
     4981 gctcgcgacg ctcgcccgcg cgcgccgcgc tctccgccac ggccacggct ctgggggcgc
     5041 ttcccgccgc cgtgggcgca ctgatccatg cgcttcccgc ggcgctcacc gacctggcca
     5101 cgcggcgaat cgccacgacg ccggcccagg tcgcgttcgt gcggatcgcg gcgggcgcga
     5161 tcttcttcat cgcgtggtac gtcgtcctgg gttgggcggt tctgttcggg atgggcgttg
     5221 gccccgagat gacggcggga gtgctcgcgg tgtgcgggct cctcggcgcg tgcgcgctcg
     5281 cctacacgcc tcgcgcgcgc gaacagctcg agcggtggcg cgtcgggtgg atcgcgttcc
     5341 gccatctgag ccttgtctgc cgggcccacc gcgaggaaga atggctcgag cgccgcgtcg
     5401 cggcactcct cgaatacgcc ctggaggtcg cggatgtcga tcctgcatac acgccggttc
     5461 tcgagctggg cgcgcggcca tctcctctgc agcgttgacg agaggccgga ccgcttcgcg
     5521 atcaccttcg acgacggacc caatcccaac gccaccgacg ccgtcctgga cgtgctcgcg
     5581 cgccacgatg cgcgcgcgac cttcttcatg ctgggggggc acgtgcgccg ccgcccggag
     5641 ctggcccggc gcgtcgtcgc ggccggccac gaggccgcgg tccacggcga cacgcactgg
     5701 ccgatgccgc tcctccttcc gggggcgatg cgggggcagg tccgccgctg cgccgcctcg
     5761 atcgaggcgg cgaccggaac atccccccgt ttctaccgcc ccccgttcgg gttcatgacc
     5821 cccggccagg cccgcttcat ccggggactg gggttcgagt cggtgctggg ggacgtctat
     5881 cccgaagacc cgcaccggcc cggggccggc cggatcgcga cgcgggtgct tcggcggctc
     5941 tcgggaggct cgatcctgat cctccatgac ggaagcccct ttggggcggc cgaccggagc
     6001 cagacggtcg aggccctggg ggcgatcctg ggggaaatgg ggcggcgggg gctccggggg
     6061 gtcacggtgg cggagctggt gggggaccga aaaattttct gaatcgccgc tttttgggcc
     6121 tccccaagtc cctggggttc aggtctatac ttggtccatg tccgccgcaa cccgggttct
     6181 ggccgtgagc gcgctggtgg tgggtctatg cccggcgctg gcgttcgcgt gcgaggacga
     6241 ggcttcggcc aaggccgaag cttccgcgaa gacggtcaag gtcgaaatct gcaaggcctc
     6301 gaccaagtgc accgtcaaga tcaaaacgtc ccgcgccgag aagtccgtga ccgtcgtggc
     6361 ggacgacacg aagtgttcgg tttccgcgaa ggcgatgaac acgcagggcg cgcaccacgc
     6421 gtgggagccg ggacacctga tcgagcccga ctcgatgaag ctcggtcccg gcgacgatct
     6481 caacgcaccg aacgccgagc agtgggtccg catctccagc aagagcggct cggttcagat
     6541 ccaccgcggc ccctgggaga aaaagacgga gggccgcacg tggatgaatg tgtacggccc
     6601 ctaccgcgtc ttcctgcagt cgcagcttcc ctagatcaca tcaccagcgt tcccgttcgc
     6661 aagagatagg cgagccgcgc ccgctgctcg ggttgcagca gggcgtgcag ccgctcgatc
     6721 gattccacga ccttccgctg gacgcgatcg gcgctcttcg tgcggagcgc cgcggcttcg
     6781 tccgccttgg cgcgatcgaa cgcatcgccc tcggaggctt cggccagcag gctcaccgcg
     6841 cggcggtcgt ccacttcggc ctgggcgcgc tcgatcttca gctggtcgag gatcgccgcg
     6901 acctgcttca tctgcgcgtc gtccagctgt agcttccagg ccaggaagcg cagcggacgg
     6961 cggacgccga ggccgggcga gtgtccgaag ccggggtagt ggtggtggga gaagtcggac
     7021 cagggttggc tcgcgcccca gcggggaccg cactgggcgc gcgccgcgtg tacggcggga
     7081 cggcacatag gatgttcctc cacgcgccgg ggccgtgctc tccgaagtct cgggagggct
     7141 cccggcgggt cgtcacgacg cgggcaccat gcctcgcgtc ggatgaatca tagcacggca
     7201 tccgggaacg gcatccggtg gggcaccgct aacccgcggg cttttgcctc gcgtcccgtg
     7261 cgcgccgctc cgcgtcgccc cgcaccagcc ccaatacctg ttggcggatc aggagaatcc
     7321 ccggcgacat cgtggcccag tacttccgga accgcgtgcg cgccgtggcg tcggtggtgg
     7381 ccacgcgcgt ctgggtgcgg aagagcgatc gctcctcgcc ggcgggatcc acctccagcg
     7441 tccagacgat cttggcccag cccggtctgt ggaaggccgt gaattgcgcc ggcgggaggg
     7501 gatggaactt caccgtggat tcccaggggc gcgtcacggc gccgaacacc atggcgcgcc
     7561 cggggacctc ggccagaagc ccccatccca tgccgcgcac ctgatccagg aacgggcggc
     7621 gggccagctc ggcgccccga ctccgcaaga ggatctttcg cgattcgaag atggcgcgca
     7681 cgagcgggga gcgctggagg tcgacgtcgc gcgcggcggc gtaggtgacc ggcgcgggcg
     7741 ctttgacctt ggtctcgtgg cgttccgaga cgtcgtactg cggaaggaac cggtccatcg
     7801 agacgacggc gcgggcgggc ggcgcctggg gtttgacgcg gccgaaccgg gtccaggcgg
     7861 cgcccacgta cacggcgtat cccagcgcgc ccacgccggc cgcgacgacg agcgcgagca
     7921 gaacccggcg caggaccctc acgccgcacc cgccagcttc tcggcgcgcc ggtgccgata
     7981 caccgaggcg aattccgcgc cgaccaggag gatcgacgag cagacgtaga cccagagcat
     8041 gaatccgagg atacccccca gcgcgccgaa caacgacgaa cggctcgcga tgatcgcgac
     8101 gtacagcccg aacaaatact tcgagatctc gaacagaaag gtcgcgacca tcgcggcggg
     8161 gaggacgtcg cgcatgcggt gggtcacgtt cggcacgaac cagtagacga agaagaaccc
     8221 cgcgaagctg aggagcgcgg gcatgctcca ctggatggtg gcccagacca ggtccggtcc
     8281 cgacggcatg ccgccggccg ggatctgccg ctcgcccagg agcacgtgca gccccgccgt
     8341 gctcgcggcg gagacgacga gcagtcccca gatcccggcc ccggtcagga agtcgcgcac
     8401 ccgcacccgc acgaagccgc ggcggccgct gactccccag gcgcgattca ggccccagcg
     8461 tgtcgcgctg atcatcccga gcgaggacca ggcgagcccc aggaccccca ggaccgagat
     8521 cccaccgctc gctttccgga tcgactcgac cgattgcagc aggaaggcgc ggttctgggg
     8581 cgagccgacg ggcaggagtt cgagcatgtg cccgaggagg tgggcctggt cgcgggggtc
     8641 gtgaatcgtc cagccgaccg agaggatcag aagggcagcc gaaggcacgg ccgaaaagag
     8701 cacgaaaaag gcgatggagg cggcatttcg ggcgccctcg tgctcctgga agcggaggta
     8761 ggtccgacgt agatttcgag ccagcatcat catggccggg agagcccctg gatcgtcacg
     8821 cgttgcgctt ggatgagacc ttgcggccaa tgctaccctc gtataccata tgacgtaatg
     8881 gcagtgtcca aacgacctca gcgcaagacc gccgccaagc ccaagaccaa gaggcggata
     8941 gctacggctg cgcccatcaa gaaggcccag tcgatgcgcc gcgtgcgcat catcctcgcg
     9001 gacgattatc cgatggaccg ggccggaatg gtttccattc tgcgctccca gccggatttc
     9061 gagatcgtgg gcgaggcctc gtcggttcgc gaggccggcg ccatgtgccg cgagatgaat
     9121 ccctccgtac tcatacttgc catgcgtctc caggcgaccg agggccgcac gccgatcgcc
     9181 tcggtccacg cggtgtctcc cgaaacgccg gtgctcgcga tcgccgagcg cgccgaaggg
     9241 cactgcctgg tgctcaatcc gccgggctcc aagcgcaccg ataccgcgca gctccccgtc
     9301 acgtgcacgc agggcaccga ctgcctgcag ctcgcggtgg ccgagggcgc gaccggcacc
     9361 atccggcgca gcgccacccc cgacgagctc ttccgcgccg tgcgcacggt cgcctcgggg
     9421 aacgcctggt acgaggcggg caccgcgacc gcgatcatgc gccacgccct ggccggcaac
     9481 ggctcggggg gcagcgcccc cgcgcccctc tcgggacgcg agctggaagt ggcggagctg
     9541 atcgccgccg gccgctccaa caaggaaatc ggctccatcc tcaacatcag cgcgccgacc
     9601 gtgaagaagc acgtcggccg aatcctcgag aagctcgggc tccaagaccg gcttcaggta
     9661 ggcctgtacg tggcgcgcaa cccgctcgtt cttcaacccc tcgataccaa gcggcgttag
     9721 ccgcccgttg ctacctaggt accactccga ttgcgcctgc gagcgcgtag actgccgcgc
     9781 gcgacacgac gatgattggt gtggtcagag caaacgtggt ggtttcctac agggagggtt
     9841 cgatgaatcg actcggtacg acgctcgcgg gagcggtgtg cgccggcgtg ctggcactgg
     9901 gcttctgggg agtcgcgagc gcggaggaaa ccgccgctgc cgccgctccg gccgcccatc
     9961 agtacgtggg tgcgaagaag tgtaagatgt gccacaacgc cgagaagggc ggggcgcagt
    10021 tcacgaagtg gacggagtcc aagcacgcga aggcgctcga gacgctcggg agcgaggaag
    10081 ccaagaagat cgcggccgag aagaagatcg cggatcccgc aacgtccgcc gagtgcctct
    10141 cctgccacca gacgggctac ggcgaagcgg cagaccacct cgccgcctcc tacgtcgccg
    10201 agggcgtgac ctgtgagtcc tgccacggcg ccggcgcgga ctacatcaag atgaagacga
    10261 tggcgggcat ccgggacaag tcgctcaagg ccgaggacta cggcctggtg atgccgacca
    10321 aggaaacctg cgagaagtgt cacaacgaca agagcccgag cttcaagtcg ttcgacttcg
    10381 ccgagttcag caagaagatc gagcacaaga ttccggaagg ctacaagcgg ggtggcgcga
    10441 cggaagaggc caccaagtag ccggctgagt ccccgagcac ccgccatatg aagcgcgcgc
    10501 ggcgcccaag gcagcgtccc tgggcgccgc gctgtatttg cttccccgtc tcggtggcgc
    10561 gcgcggccgc gctgtcgtgg atcgccggcc tggccgtcgt cgtggccctc ctggccttcg
    10621 tgccggccgg cgcggccgcc cccgcctccg tccgaatccc cgactccgtc tgcatcgaat
    10681 gccacaccgg cgccaacctg gacagcgccg tcgtccgcgg gcggaaggtc aagctcgaca
    10741 tcgaccgcgc cgtgctccag cactcggcgc acgccggcgt ggcctgcacg caatgccacg
    10801 tcggtttcaa ccccgaagag cagccgcatc gcgcgcgcgc cgttccggtc gactgccgcc
    10861 gctgccacat gaacgccgac tccgcgcacg tcttccacgc gaagacgacg ttcgcgaagg
    10921 cggcgggcgc cgtcggctcc tgtcaggggt gccacggcga ccacgacgtt ccgaaggctg
    10981 gcgcggcgac gacgccggcc ggcgcgcggt cgctggacct cgcgtgcgcg cgctgccatc
    11041 gcgaggaagt cgcgcacttc gagaactccg cgcacggccg cgccgccgcg cgcggcgagc
    11101 gggcggcgcc gacgtgcctc tcctgccacc ggcttccggt caccgcggcg cgggttcacg
    11161 accagcgcga cgcgaggctc aagctggcgc aggagcagct ctgcctccac tgccacctgg
    11221 acgatcccgc cgtgcgctcg cgcgtcgggc cgcagacggg gttcatccgc gcctacgaga
    11281 ccagcatcca cgggcaggag ctccggcggg ggaacgggaa ggcggcgacc tgcatcgact
    11341 gccacggcag ccacgagatg gcgcgcggct tcgagccctc cgcgaaaacg gagaagttcc
    11401 acatcccgga cacctgcggc cgctgccacg acaagatcgc ggcgacctac aggacgagcg
    11461 tgcatggaca ggggatggcc aagggcaacc ccgacacgcc ggtctgcacc gactgccacg
    11521 gcgagcacac gatcaagaag gccgacgcgc cggagtcgcc ggtcgcgccg gagaacgtct
    11581 cggccgaggt ctgctcgccc tgccactcgt cggtgcgcct cgcgaccaag tacggcatca
    11641 agagcgaccg cttccagacc ttctccgatt cgttccatgg actcgcgatc cgcggcgggc
    11701 aggtctccgc ggcgaactgc gcctcctgcc acggcgcaca cgacatcctt ccgtccagcg
    11761 acccgcgcag ccggatcagc cccgcgcggc tcgcggtcac atgcggcaag tgccactcgg
    11821 gcgccaccgc ggccttcgcg cgcggcaagg tacatctcga tgtcacccgg acgaaggagc
    11881 cgctcctcta ctggatcggg ctcatctaca cgatcctgat cgtcggggtc gtgggcggga
    11941 tgttcctgca caacgcggtc gacttcttcc ggaaggcgca gcacaagctg cgcgtgcggc
    12001 ggggcctggc ggcggcgcac gagccggaga gccgcgcgct gtacgtgcgc atgaccggaa
    12061 gcgagcgggc ccagcacgcg accctgctca cgagcttcac cgtcctcgtg ctgaccggct
    12121 tcatgctgcg ctatcccgaa tcgtggcccg tggagcagct gcgccacatc tggcacaacc
    12181 tgttcgaggc gcggagcgtc atccatcggg tcgccggggt ggcgatggtg gcggcctccc
    12241 tcttccacct gtactacctg gggttcacga agcgcggccg ggagttcttc cgcgacatgc
    12301 tgccgcgtcc gtcggacctg agagacgtga tcggcgcctt gcgctattat gccggcctca
    12361 cccggcagcg tcccaagttc ggccgcttct cctacatcga gaagtcggag tactgggcgc
    12421 tggtgtgggg cacggtcgtg atggcgggaa ccggcatgat cctctggttc caggatccgt
    12481 tcatcgccct cctgaccaag ctgggctggg acgccgcgcg atcggtccac ttctacgagg
    12541 cgatcctcgc gacgctggcg atcctggtct ggcacatcta cttcgtgatc ttcaacccgg
    12601 acgtctaccc gatgaacctg gcgtggctga aggggacgat caccgaagaa gagatggagg
    12661 acgaacaccc gctggagctg gagaagatcc ggcgcgagcg cgaggcggac aaggagggcc
    12721 gatcgtgagc gagaaggacc ccggacagga gaaggacccc ggacaggaga aggaccccgg
    12781 ccaggagaag gaccccgaac gtcgcggcgc cctcgtgtgg ctggtccgcg gattcctcgc
    12841 cctctgggcc cccgcggcgg caggcatggc cgcctccttc ctgaaagccc cgtctcccga
    12901 gaaccgcccc ggcgagagga tgctccgcgg gggaccgatg tcgtcgatcg ccgtggggga
    12961 cgcccgattg gtgcggcacg gcaccgaccc ggtctacgtg gtgcgcgttt ccgaggagca
    13021 ggtcgtggcc gtctcggcga tctgcacgca tctccgctgc gtggtcaagt ggaaccgcga
    13081 ctccggcacc ttccgctgcc cctgtcatga cggcgcgttc gatcgcggcg gcaacgtgct
    13141 ctccggcccg ccgaagaagc cgctccagca attccccgcc gaggtccggg cggacgagat
    13201 cgtcattcac acctaagcgg agatccttgt ggaacggaat cgcggcggcc ttctcggctg
    13261 gctggaagcc cgtgtcaatc tgacggagat cgtctccttc ctcacggtgt tcggtctgct
    13321 ccccgtggag ctggactcga ggaagccgct gaaggaggcg ctgcgcgagg cgatggcgca
    13381 gccgctccct tcgtacgcgc gctggccgca ggtgatcggc gtcctctcgt tcatcctgtt
    13441 cgtgatcctg gccgtcaccg ggacgatgct cgcgttctac taccagccca cggcggacgt
    13501 ggcctatccc tcggtgacgt tgatcgcgcg cgacatctcg ttcgggaacc tgacgcatca
    13561 ggtccaccac tgggcggcgg tgctcttcct gatcctcctg gcggcgcggg tgattcgatt
    13621 cttcttcgcg cggctctacc accaggggcg cgaggtcgtg tgggtcgtcg ccgtcctgac
    13681 cttcgtggcg gcggccatcg cggacctgac gggacggctc ctgccgtggg acgcccgcgg
    13741 ctactggacc acggtgcggg cgcgcgaggt gaccgacgcg ctgccgatcc tgggtccctt
    13801 cacctcgttc ttggttggcg gaagcggcct cgactcgctg gtgctgacgc gctactacgt
    13861 gctgcacgtc gtggtcttcc cgctgctcct gatcggactc ttctatctcc acttctcgag
    13921 cgtgcggcgg gtggggctct cgcgcaacgc gccgacgggt tcgggcgcgg cgcccacccc
    13981 cgtcggcccg cgagcgctgc acgtggtgac ctacgatctg atcctgctca tcatcctgat
    14041 gatcggggcg ctgctcacct tcgccgtcct gatgccctat cccttcgacg tcgtcgctga
    14101 tcccttcacg acgcctccca acgcgcgccc gccgtggtat ctcctcgcgc cgcacgcgct
    14161 gttggaggcc ttcccggcat gggtgccgcg gccgatccgg ggcatcctgc ccgaggcgat
    14221 gctcctggcc gtgatcctgg tcccgatgat cgatcgcggc acggtcacgg atgagcggcg
    14281 gcggaagctg ttccggaaca tcggctactc ggttctcgtc gtgtgggtgc tcctgacctt
    14341 gatcggctgg atgctggagg tgcggcggtg agcgcgcggt tgggcgtacg ggccgcggcg
    14401 atgagcgcgg ggcgggccgc g
//
LOCUS       DASSLZ010000298         4142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1503879, whole genome shotgun sequence.
ACCESSION   DASSLZ010000298 DASSLZ010000000
VERSION     DASSLZ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503879"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFM00_11305"
     CDS             <1..649
                     /locus_tag="VFM00_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HET7498877.1"
                     /translation="WPGAAASFLGMWTVMMVAMMLPSLVPTLWRYREAVGRRVDVRPD
                     KLTALVAAGYFTVWTALGLIAFVLGVALANVEMQQPALASAVPLLAGGAVMAAGILQL
                     TKWKARQLACCRQATGLIGALRQGRDNDFALAADFSTAWRQGLRLGLRCCYCCAALTT
                     ILLILGVMDVRTMAIVTAAITAERLAPGGERLARIIGIAVVSVGLFLIARALMPA"
     gene            827..2146
                     /locus_tag="VFM00_11310"
     CDS             827..2146
                     /locus_tag="VFM00_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498878.1"
                     /translation="MKRLAFVVFSLVMSLSFLPRASSAQGPCAIDAPSRVGIDQAFTL
                     CGPTGGGYRYEWYGPGLDSDAGTRCVTARVGDSGTYEYLLVIKRNGQELDRCKKVVNV
                     GNSTGGAGSCTITGPTSIQAGQTARLCAPDDGLHIYRWTGPNNFTSTSPCVNVTEEGT
                     YYLTSRNRLTGSSRECTHRLDVVGAGGGGESDCISGPTTIPRNGTVELCAPSRGVASY
                     RWTGPRGFTASERCVTIDEPGTYTVSIRGGFTGREERCSQTVRSGGWGGGGGGGGGGW
                     GGNDNGDENPDDVVWDNCPRPLQFWRNLYDDANGSGDLSSADLRAIARWVDDHSSYFN
                     WRDDLQGMRQALLPGSPMTRSKQLARQYAALLANVAAGELGATPEGRNQISLDLDTQV
                     DFAGASTLRELISMTDRVLSARRGSFSRLNAKLTQINRGQGIGPVCD"
     gene            2580..>4142
                     /locus_tag="VFM00_11315"
     CDS             2580..>4142
                     /locus_tag="VFM00_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7498879.1"
                     /translation="MTDPTPADEYQLIVQTSSMLRKRGTREPEKAMQVYLRGCAELFR
                     AEAACIVSAEVGRPTRVEYSIPQETAWDLSLLDDLARGTAEEIPAGILAARLRRRGRT
                     WGAIALRRDGSDFDVAERRALSRIAAEIDYFVGTLDDASLAEVRARIDMKILRDLPPK
                     DLYYQILDGLHRLTRYDHSASLFLYDSPSGRLELVAEQIAWRKMRSERIGEIRTLPDE
                     LQGLIREDVVLGFHRTENRWAEWTRSGGDGLAAVLDPNVPSRNGSDRAGAVNPEEPEP
                     PLQAMLVAAIGSTRDGFGLLRLGAQRPNAFGDHELGIVRSFVPAVSVALRRAQTSEEV
                     QRKVLDVERRATLAHLARGVAHDVNNAFGSILPLVQQMRVEEEEGRLDPVRLRQDLAQ
                     IESSLDVARRIFTGMLRLAKGSVSPGATCEPGRAIASACGALGGLMSRAGVLLECELP
                     ENLPPVVGKQYELEQLFLSLATNAIEAMPTGGALRITGRVDGERVWIVVADTGHGIPP
                     DLLPEIEKPFVST"
BASE COUNT          691 a         1457 c         1353 g          641 t
ORIGIN      
        1 atggcccggc gccgcagcat cgttcctggg catgtggacc gtgatgatgg tcgcaatgat
       61 gttgccgtca ttggtcccga cgctgtggcg ttatcgcgaa gcagtgggaa ggagggtcga
      121 tgtccgcccg gacaagctga ccgcgctggt ggccgcggga tactttaccg tctggaccgc
      181 actcggcctg atcgccttcg tcctgggggt cgcgctggcg aacgtcgaga tgcaacagcc
      241 cgcgctggct agcgccgtcc cgctcctggc cggcggagcg gtcatggccg cgggaatcct
      301 gcagctaacc aagtggaagg cccgccagct cgcctgctgc aggcaggcta ccgggctcat
      361 tggcgcgctc cgacaaggcc gagacaacga cttcgccctc gccgccgact tcagcacagc
      421 ctggcggcag ggtcttcgcc tcggcctccg ttgctgctac tgctgcgccg ccctgacgac
      481 cattctccta atcctgggag tcatggacgt tcgcaccatg gccatcgtca ccgcagccat
      541 caccgccgaa cgcctcgccc ccggaggcga gcgcctggct cgcatcatcg gtatcgccgt
      601 cgttagtgta ggactgttcc tgatcgcgcg agccctgatg cctgcatgac cgtcagtgta
      661 tgccaaaccg agcggcgggc gccctgtaac aacctccaca gtgagtccta gaactcacga
      721 tccccctggc gactcgaccc gctaaacgat tgcaccgcaa tatcggcctg ccctggcacg
      781 gaccatgccc ttgtggcgca gaagtgccac caggaggaac gaacccatga aacgcctagc
      841 cttcgtggtc ttttccctag tgatgtcgct cagctttctc ccccgcgcct cgagcgcgca
      901 gggcccgtgc gccatcgatg ccccgtcgcg cgtcgggatc gaccaggcct tcacgctctg
      961 cggccccacc gggggcggct accgctacga atggtacggc cccgggctcg attccgatgc
     1021 cgggacgcgc tgcgtgaccg cgcgcgtcgg ggattcgggg acgtacgaat atctgctggt
     1081 catcaagcga aacggccagg agctggatcg ctgcaagaag gtcgtgaacg tgggcaactc
     1141 cacgggcggc gcgggatcct gcacgatcac cggcccgacc tcgattcaag ccggacagac
     1201 agccaggctg tgcgccccgg acgatgggct ccacatctac cgctggacgg gacccaacaa
     1261 cttcaccagc acttcgccct gcgtgaacgt gaccgaagag ggcacgtatt acctcacgag
     1321 ccggaaccgg ctcaccggaa gctcgcgcga gtgcacgcac cgcctggacg tcgtcggcgc
     1381 cggcgggggc ggcgagtccg actgcatctc gggcccgacc accattcccc ggaacggaac
     1441 ggtcgagctg tgcgctccgt cacgaggcgt cgcctcgtac cgctggaccg gcccgcgcgg
     1501 cttcacggcg tcggaacgtt gcgtcaccat cgacgagccc ggcacctaca cggtgtcgat
     1561 ccgaggcggg ttcaccggcc gtgaagagcg gtgcagccag accgtgcgaa gcggcggctg
     1621 gggcggaggc ggcggtggcg gcggaggcgg ctggggcggg aacgacaatg gcgacgaaaa
     1681 cccggacgac gtggtctggg acaactgccc gcgtccgctc cagttctggc gaaacctcta
     1741 tgacgatgcc aacggaagcg gcgacctttc ctccgccgat cttcgcgcca tcgcgcggtg
     1801 ggtcgacgac cattccagct acttcaactg gcgggacgac ctgcagggca tgcggcaggc
     1861 gcttctgccg gggtcgccca tgacgcgcag caagcagttg gcgcgtcagt acgccgcgtt
     1921 gctggccaac gtcgccgcag gagagctggg cgcgacgcct gaaggtcgga accagatcag
     1981 cctcgacctg gacacgcagg tcgatttcgc gggagcctcg acgctgcgcg agctgatctc
     2041 gatgaccgat cgcgtcctca gcgctcgccg cggcagcttc tcgcggctca acgcgaagct
     2101 cacgcagatc aatcgaggcc aggggatcgg ccccgtgtgc gactgagtcg cgtcaagacg
     2161 agctaagaga gagggcccgg accagcaacg gttcgggccc tttccatctc cgcgtcccga
     2221 agattctccg cacctcaaag cagagcgtca attcactgcg acaatcgtca atgatttcac
     2281 cgccgctggc ggaagttctt tatatgttaa gccgatcaca cgaccggccg acagccccca
     2341 caagcgtcgc tgcggagcga caatcgacgt caatttcacc cgcgccagcg cggtacatat
     2401 tattttctcg caagacggcc tcgcgcccgt gcacttccga ccccgcggct gcctcggcct
     2461 cgcgcgcgag atcggggccg accgccgacc aggattccct ggcgacccct cccctcctgt
     2521 tctaagctcg ccgggtccac tcccccatct gacccgagcg cgatcccggc ggagcccacg
     2581 tgaccgaccc gaccccggcg gacgaatacc agctcatcgt gcagacgagc tcgatgcttc
     2641 ggaagcgagg cacgcgcgag cccgagaagg cgatgcaggt ctatttgcgc gggtgcgcgg
     2701 agctctttcg ggcggaggcg gcgtgcatcg tctccgctga ggtcggccgc ccgacgcgag
     2761 tcgagtacag catcccccag gagaccgcgt gggatctgtc gctgctcgac gacctggccc
     2821 ggggaacagc ggaagaaatc ccagccggca tcctcgccgc gcgcctacgg cgtcggggcc
     2881 gcacctgggg cgcgatcgcg ctccgtcgcg acggatcgga cttcgacgtc gcggagcggc
     2941 gggccctctc gaggatcgcg gccgagatcg actacttcgt cggcaccctg gacgacgcca
     3001 gcctggccga ggtgcgggcg cggatcgaca tgaagatcct gcgcgacctg cctcccaagg
     3061 acctctacta ccagatcctc gacgggctgc atcgcctcac ccggtacgat cattcggctt
     3121 cgttgttcct ctacgactcc ccctcggggc gcctggagct ggtcgcggag cagatcgcct
     3181 ggcgcaagat gcgaagcgag cggatcgggg agattcgaac gctccccgac gagctgcagg
     3241 gattgatccg cgaggacgtc gtgctcggct tccatcgcac cgaaaaccgc tgggcggaat
     3301 ggacgcgatc gggcggcgac gggctggcgg cggtgctcga tcccaacgtc ccctcgcgga
     3361 acggctcgga ccgcgccgga gccgtaaacc ccgaagagcc ggagccgccg ctccaggcca
     3421 tgctcgtggc cgccatcgga tccacgcgcg acggcttcgg cctcctgcgc ctgggagcgc
     3481 agcgtcccaa cgccttcggc gaccacgagc tggggatcgt ccgaagcttc gttccggccg
     3541 tctccgtcgc cttgcgccgc gcccagacca gcgaagaggt gcagcggaag gtcctcgacg
     3601 tcgagcggcg cgcgacgctc gcccacctgg cgcgcggcgt cgcgcacgac gtgaacaatg
     3661 ccttcggatc catcctgccg ctggtgcagc agatgcgcgt cgaggaagag gaggggcgcc
     3721 tcgatcccgt ccgcctccgc caggacctgg cccagatcga aagctcgctg gacgtggcgc
     3781 gccgcatctt caccggcatg ctccggctgg cgaagggcag cgtttcaccc ggcgcgacct
     3841 gcgagcccgg gcgcgccatc gccagcgcgt gcggcgcgct cggcggcctc atgtcgcgcg
     3901 ccggcgtcct cctcgaatgc gagctgcccg agaacctccc gccggtggtg ggcaagcaat
     3961 acgagttgga gcagctgttc ctgagcctcg ccaccaacgc aatcgaggcg atgcccaccg
     4021 gaggagcgct gcggatcacc ggccgcgtgg acggcgagcg cgtctggatc gtggtggccg
     4081 acaccgggca cggcattccg cccgacctgc tgcccgagat cgagaagccc ttcgtgtcca
     4141 cc
//
LOCUS       DASSLZ010000299        17892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1631323, whole genome shotgun sequence.
ACCESSION   DASSLZ010000299 DASSLZ010000000
VERSION     DASSLZ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 17892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17892
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631323"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(567..1205)
                     /locus_tag="VFM00_11320"
     CDS             complement(567..1205)
                     /locus_tag="VFM00_11320"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006956724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET7498880.1"
                     /translation="MSRSRLARFDDGYREQWGELVAGVDEAGRGPLAGPVVAGCVVLP
                     ADARGLRGVRDSKEMTAEEREEAVVKIRAVALGVGVGVASVEEVDAFNIRMASLLAMR
                     RAVDALGITPQGFLVDGKDVFSMDLRCAAVVDGDAKSLSVAAASIVAKVARDAMMIEQ
                     ATFHPMYAFESNKGYGTPEHLSALREHGPCAIHRRSFRPVWETLYEQVTLEI"
     gene            complement(1252..1614)
                     /gene="rplS"
                     /locus_tag="VFM00_11325"
     CDS             complement(1252..1614)
                     /gene="rplS"
                     /locus_tag="VFM00_11325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET7498881.1"
                     /translation="MSAIERFETRFTTDRTLDFRPGDTIKVHVRVIEGEKERSQVFQG
                     VVTNIRGSGMRTSFTVRKISAGIGVERTFPLHSPSVAKIEGARKGKVRRAKLYYLRGR
                     KGKAAKVTERESLGGDEA"
     gene            complement(1611..2357)
                     /gene="trmD"
                     /locus_tag="VFM00_11330"
     CDS             complement(1611..2357)
                     /gene="trmD"
                     /locus_tag="VFM00_11330"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET7498882.1"
                     /translation="MRVSILTLNPGFFGGALDEGMIRVAREKGLLEVVLVPIRAFADD
                     RYGTTDDYPYGGGAGMVMKAEPIVAAHESVARSCGGAAPRTLVTSPQGRVLTQVWVRE
                     LAAEKHLAVVCGRYKGIDERVVETLGAEEFSIGDYVLSGGEPAALVLMDAVSRLQPGV
                     LGDAESAEADSFSEALLDAPVYTRPDEFRGRSVPEVLRSGNHEEIRRWRRREAIRRTL
                     ARRPDIVRGRAWSEEDRRLMDEIKKEADQP"
     gene            complement(2364..2915)
                     /gene="rimM"
                     /locus_tag="VFM00_11335"
     CDS             complement(2364..2915)
                     /gene="rimM"
                     /locus_tag="VFM00_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET7498883.1"
                     /translation="MNPGVDEVGDAARVLVGIVAKAHGLRGEVVVKVMSDAPERFAPG
                     SEMTAAAPESLHARPLRVATSRPFQGRLLVRFEGIESREEAETIHGQELTIARSQVTP
                     LPEGKHYQFELVGLTVRTTGGEPLGRVTDIFSTGSNDVLVVDDDENEILIPMLEGVIV
                     SVDLEGKSLVVEPPPGLPGIGGE"
     gene            complement(2957..3193)
                     /locus_tag="VFM00_11340"
     CDS             complement(2957..3193)
                     /locus_tag="VFM00_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET7498884.1"
                     /translation="MKAKALIEYLAGQLVDHPEGVHVDEHDTGETTVLTLRVSPGDLG
                     KVIGREGRTAHALRALLTASVTAQGRRAILEIAD"
     gene            complement(3477..3701)
                     /gene="rpsP"
                     /locus_tag="VFM00_11345"
                     /pseudo
     CDS             complement(3477..3701)
                     /gene="rpsP"
                     /locus_tag="VFM00_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            complement(3745..5073)
                     /gene="ffh"
                     /locus_tag="VFM00_11350"
     CDS             complement(3745..5073)
                     /gene="ffh"
                     /locus_tag="VFM00_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET7498885.1"
                     /translation="MFEDLTERLGGVFRKMLGRGRLSDQDVKDSLREIRRVLLEADVN
                     LNVARDFLKSVEARAVGSDLLQSVQPGQQVVKIVHEELVKLLGGAGPQTGLQFPANRA
                     AVLLLAGLQGSGKTTTAAKLARFWMKREKRVLLAALDLQRPAAVDQLEVLGKQVGAPV
                     HLDRAAKDPVALAHEARRRADKEGFDLLIADTAGRLHVDEELMTEVERVHKKLEPQES
                     YLVLDGMTGQDAVSIAESFTARLPITGFVLTKMDGDARGGAALSLRSVTGKPIRYLGT
                     GEKLDGIEPLHPDRLASRILGMGDVVTLVERVQERIDLKKAEDLEKKLRKQKFTLEDF
                     LGQLQEMKKLGPLEEIMKMIPGMKLPAGAQVDERELKRTEAIIQSMTRGEREEPRVID
                     GSRRRRIAKGSGTTVQDVNRLLKQFEEMQTMLKRMGKMPKGRFPMGMPRR"
     gene            complement(5243..7132)
                     /locus_tag="VFM00_11355"
     CDS             complement(5243..7132)
                     /locus_tag="VFM00_11355"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7498886.1"
                     /translation="MRPGRSPLGVLALLLFSFALHSPPVSAQSFCDFAPRVSTSGIRA
                     AVGVQAVVEVTVLDPNGDGILSLTANTSALPAGNNAVFTTNASNTAGTLTWTPQAGQT
                     GSFSVSFTASNALSATASATINVTDTAGAPFVQCPSTATTGEFRPLSFHVTAGDPDGE
                     PIVSLTAAPLPNHATFTVNATNTAGTFDWTPDFTQAGSYNVGFTATSSASATYNTSIT
                     VSSVDRAPVVNVPPSATGSETVPFSVCAAATDPDGDAIISLTASPLPTGATFTSSASN
                     TSGTLNWTPSPGQAGIYNVTFIASNTLSGSATLPITIKSGDRAPVVTAPAAASGAVGT
                     PIQFTVSAADPDDTPIESLTASNLPQGATFTPNLANTVGAFDWSPSAGQGGTYFVLFV
                     ATSHGLTGSATTAVNATGDLLAATAFTTGGNGTIRLGSGKAAWCANVEPVSGDFAVTD
                     VVPTSIVLISNGTGSVSQIPAIAGKGIVAGDANRNTVADFEVCFAKTDLRQLFSNLTG
                     QSTVTVTIRGDLTGGNRFEAALSVDVIAGGRNAVALLPNPPNPGASILFSTSRPGPVR
                     ARVFDIRGRLVRTLSASREVFAGEHALAFDGRGDSGKPLPSGIYLFRIETPDGKSTTK
                     AAIVR"
     gene            7809..8018
                     /locus_tag="VFM00_11360"
     CDS             7809..8018
                     /locus_tag="VFM00_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498887.1"
                     /translation="MLIAVFDERATRVKVIFDADEPATLSGTFRLPVIPWDFTDAEGH
                     RVPSGTYRLYFKAGSFISTSDVDVP"
     gene            8015..8692
                     /locus_tag="VFM00_11365"
     CDS             8015..8692
                     /locus_tag="VFM00_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498888.1"
                     /translation="MRRLLAAASALACFAWAGGAPAQTASPDTTAQAPPGGATVPSVS
                     SDTTAAPVSAAPADTTTPSPVTAARAARVAVGKDNDRFELGAAVADGPFDLLGTFGYE
                     RYVQRGGPFEHWIHVEVSGGATPFLNEGTLSVAYLLRPLGTIRRTWAIRPILEAGPGA
                     HLVVQVAEIQGFGETAFHSRVYVKTHAIAGLEALLGRRWGVVVRGRFSVPAHHPFDYA
                     QIALFRR"
     gene            complement(8662..11460)
                     /gene="uvrA"
                     /locus_tag="VFM00_11370"
     CDS             complement(8662..11460)
                     /gene="uvrA"
                     /locus_tag="VFM00_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7498889.1"
                     /translation="MPRREFVAPDTHLRLFGARQNNLKGIDVSFPHAALSVITGVSGS
                     GKSSLAFDTIYAEGQRRYVECVSTYAKQFLDRLPRPEIDRIEGLSPAVAIRQGAAAQT
                     GRSTVGTVTEIADYLRLLLARVGETRCGQCGTVVPRYSVDSVLEAALRFEGEDLIVAA
                     ALPARPGERPPQIWARALAAGFVRAQPRGAKSWLRLDEPLSAGSKGPIRVMIDRVRAG
                     AENRMRLREAIELAWREGEGRLDIERPSGEVLTFADGRTCVTCGRTFPDPRPQLFSFN
                     SPYGACPECRGFGNVLTFTLERVVPHPEKTVREGALDPWKNSWRAYFMPKLEALSKER
                     GISLDVPFASLPEEQQRVLLHGAPGFRGVFPFLERLQLKSYKSSARFLVKHYQEAMPC
                     ALCGGTRLKPEAMEVHVGGKNVAQLLGATVGELRAFFAGLRLDPVREAVAASIRTEVE
                     GRLEYLGEVGLDYLTLDRASKTLSGGESQRIELANALGGSLSHALYVLDEPTVGLHPR
                     DTERLIRVIRRLRERSNTVIVVEHDPDVIGAADWIVELGPGAGQRGGALLYAGPVQAW
                     PSRAEATEPPEMAAEGRASYGEAGTAAKGKKPKGTWIEVRGAREHNLRDVSARFPVGA
                     LVGVCGVSGSGKSTLIEDVLWRAAARALDQDAPAPGAHDAVLGLQPFRRVAMVDQSPV
                     ARSARSNPATYVKAFDRIRERYARTPLARQRKYTPGTFSFNVAGGRCEACEGAGVTRV
                     EMYFLADLWIPCEACEGRRFRPEVLEVTVHGLSIDALLDKTVEEAIQLFRDDPDVAAP
                     LWVLERVGLGYLRLGQPFSTLSGGETQRVKLARELSDRAAGETLYLFDEPTVGLHRKD
                     VQTLLRVLRELVRRGGTVIAVEHNLDFLAACDWLVELGPGAGEEGGRITAEGPPAAIA
                     RDERSATGAYLRKSAICA"
     gene            complement(11624..12010)
                     /locus_tag="VFM00_11375"
     CDS             complement(11624..12010)
                     /locus_tag="VFM00_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7498890.1"
                     /translation="MHKSVLIVDDEQLLARTLSTVLREAGYRTVVAGSAEEAERHVFG
                     ESPMDLIVVDVRLPRESGIEMVKRLRERSLRSKVILMTAYETPEVKTEARRLKVDRYL
                     KKPFDLTALVDEVRSLIGPGGNSTPG"
     gene            complement(12373..13776)
                     /locus_tag="VFM00_11380"
     CDS             complement(12373..13776)
                     /locus_tag="VFM00_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7498891.1"
                     /translation="MILRVRIADDEELIRKSLTKLLAAEGYQVDSVATAAEVLDSVRR
                     NPPHVLVLDLRLPDGSGLDLLPRLKSLAPDLKVVVITAFGDLPTAVEAMRRGATDFLK
                     KPYEMEEVLLAMERLKAGVVRETQLDAFRRGELETFLKTRIVSESPAMGRIWDLTGKV
                     AQSESTTVLIEGESGTGKELVARAIHFESGRRDAPFLALNCSSFQEQLLENELFGHER
                     GAYTDAREPKRGLVELADEGTLFLDEVGDLPAATQAKFLRFIEDRTFKRVGGSTDLAV
                     DLRLIAATNRDLEQLVQAGKFRQDLYYRLKVVSILLPPLRERGDDVLLLTRHFLSFYN
                     EKFRKSFVSITPEVEGILRAYRWPGNVRELKNLLERIVLLENDEALREDHLPAEMMAQ
                     VESLPRVLREALAARGEGDAEMPTLAEVEQEHILKVLESTQGNRSHAARILGISRQSL
                     IERLKRIAATRGSLSST"
     gene            complement(13778..14890)
                     /locus_tag="VFM00_11385"
     CDS             complement(13778..14890)
                     /locus_tag="VFM00_11385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7498892.1"
                     /translation="MARLETPEIERELFLQALDALDTGLLALDEDRRIVALNETLARG
                     WGVDRDEVTGKPLSEVFHPETERWYLPERGARAEGRATRETRGAVADREVLMRYTSTP
                     LGDSGALLIRVEDLVDADTEEEVFRNTERLISLGELSARVAHEIRNPLTGVRTTVQFV
                     ASKLRAGDSRRDDLHDVLKELDRIEQIITDLLLFARPQAARPVATDLREVVEKVLDNL
                     ARRLEDASVEVERELDPDPPVPQVFVDPDMAQQVVLNLVINAIQAMPEGGTLRASIGL
                     RRTRYKKAYVDVSITDSGPGIPEDVKEKIFDPFFTTRSMGTGLGLSISLQIAREHGGN
                     LTARNLTQGATFRFSLPVPLPPAEPAAAEEAKEEKA"
     gene            15087..16916
                     /locus_tag="VFM00_11390"
     CDS             15087..16916
                     /locus_tag="VFM00_11390"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET7498893.1"
                     /translation="MMQSLRDNMKLIIWITAIVFLVGFGILQLGGVFGNQPTQQRGPT
                     GIVADINGEPIRYDEYMGTVNQMTRQLEQTRPLQAGEDAYIREQAWQTLVRNKLLQQE
                     AKRRHLGATPDEIKMALRVAPPEFLVQADAFKGANGQFDYRKYLAELDNPNSQMPWGQ
                     VEALVADQLPIQKLQEQIVAGAKVSEGDVRERFLLQNDKVKFKAIQFLPDSFPVDTSR
                     IGGADVESYYKAHPDEFTGPEEVKVAVVLVPRKPDATDFSTEKERLRAVLDMARAQPD
                     SFPSLARSYSEIQSASRGGDPSSEPFFDELRPIFRRGLQNLQAGQITDILQEERSLHI
                     FKVEKRWVDPATKREKIKYREIAVRVEPGTNAVRAARELAQKAEKDAKRSGLEAVATR
                     MGTRTFTSEYFAYGQSGNDVLQRFPEVETWMFRSKAGAVSEIVPTENGYFIFQVVDHR
                     PAGLRPLDRVAADAKAKLVRSLRMQRAKEAADQARAALAGGAAEPEVAARFHGRVADV
                     PEATRNGYLSTVGGREPRVIGAVFAMPAGAPWSAPLVGDNGVYVAQVEAHMAPSEDDF
                     RKQEAQVRDQLLNERRQLIFVEWMQDLRRKAKIKDYREQYFEV"
     gene            complement(16966..17520)
                     /locus_tag="VFM00_11395"
     CDS             complement(16966..17520)
                     /locus_tag="VFM00_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7498894.1"
                     /translation="MQIDSLEKLFVDELKDVYSAEKQILRALPRMSKAASNDELRKAF
                     DNHLRETEKQVDRLDQIFKDLGKSPTGKKCMGMEGLIEEAKELLSEDVEEEVLDAGLI
                     SAAQRVEHYEMAAYGCLKTYAGLLGNQKAARLLEETLDEEKRADELLTQIAERTINVE
                     AMEAGTGAGATAERGGSSKRGSNR"
     gene            complement(17538..>17892)
                     /locus_tag="VFM00_11400"
     CDS             complement(17538..>17892)
                     /locus_tag="VFM00_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7498895.1"
                     /translation="RRVLLRGRAPLERDALFFSTGNDQSCDLAIRLGCRLTLKAAIRT
                     NRRERTNVPGVYVAGDASWDVQFVVVAAAEGAKAAVAINRELQAEERVLAAENRGIRV
                     APRRPSRTGNATRKH"
BASE COUNT         2716 a         6344 c         5865 g         2967 t
ORIGIN      
        1 ggcccgggcg cgccagcacg ggccgcgctc gcggattttg ccggcgccgc cctcgcgatc
       61 ttcaccgccg ccgtgctcac ggacttcaac ggcgccgctc tgagatgccc ccgctcgcgc
      121 accggccttg cggccctcgg accgcttccg catcacccac ttcattccga cctcctcccc
      181 aacacccggt gccccgcagg gggtaaacga acgtttccct cgctggcgag ggtatgtttt
      241 ggccgctgtt cgcaaggagg agactcttgg ggggcatgct tcaattcgcc ccggggcgag
      301 ttcggggtgc atcgttcgcc gtgggaggag ttgggcgagc cggggcaacg aactagccct
      361 ggcgcaagtt ggtgttcagg ccaacttgcg ctggcgcaag tcctgttatg tcaacttgca
      421 ccggggcaag ttaagttaaa tattaactgg ctcctggcga gttgaacgtg cggtgcggcc
      481 gtccggcctc ggctacgttg cccgcaggtc cgggcaatcg gcgccaatct cgtatccgct
      541 gccgctttcc gcagcttcgc tgcggttcat atctccagag tcacctgctc gtacagcgtc
      601 tcccacacgg ggcggaagct tcttcggtga atcgcgcagg ggccgtgctc gcggagggcc
      661 gacaggtgtt ccggcgtgcc gtagcccttg ttgctctcga acgcgtacat gggatggaac
      721 gtcgcctgct cgatcatcat cgcgtcgcgg gcgaccttcg ccacgatgga ggcggcggcc
      781 acgctcaggc tcttcgcatc gccgtccacg accgcggcgc agcgaaggtc catgctgaag
      841 acgtccttcc catccacgag aaagccctgg ggggtgatcc ccagggcatc gacggctcgt
      901 cgcatcgcca gcagggacgc cattcgaata ttgaaggcgt cgacctcctc cacgctcgcc
      961 acacccaccc cgacgcccag cgccaccgct cgtatcttca cgaccgcctc ttcgcgttcc
     1021 tcggcggtca tctctttcga atcccgcacg ccccggagac cgcgggcgtc cgcgggcaat
     1081 acgacgcacc ccgcgaccac cgggcccgcc aaggggcccc gccccgcctc atccaccccc
     1141 gcgacgagct cgccccattg ctcgcgatac ccgtcgtcga atcgagccag cctgcttcgg
     1201 ctcatcgcat ccctgcggct cgggctcctt tcccaacggt acgtctagcg gctacgcctc
     1261 atcgcccccg agagactcgc gctccgtcac cttggccgcc ttccccttgc gtccgcgcag
     1321 atagtacagc ttcgcgcgcc ggaccttccc cttgcgcgct ccctcgatct tggcgaccga
     1381 cggcgaatgg agcgggaacg tgcgctccac gccgatcccc gcggagatct tgcgcaccgt
     1441 gaacgacgtg cgcatgccgc tcccgcggat gttggtgacg acaccctgaa aaacctgcga
     1501 ccgctccttc tcaccctcga tgacgcgaac gtggaccttg atcgtgtcac ccggacggaa
     1561 gtcgagcgtc cggtcggtgg tgaaccgggt ctcgaaccgt tcgatggcgc tcatggctga
     1621 tccgcctcct tcttgatttc gtccatcagt cttcgatcct cctcgctcca ggcacggccc
     1681 cggacgatat cgggccggcg cgcgagggtc cgtcggatcg cttcccgacg tcgccagcgg
     1741 cggatctctt catggttccc gcttcggagg acttcgggga cggaacgccc ccggaattcg
     1801 tccgggcgag tgtacacggg cgcgtccaaa agcgcctcgg aaaaggagtc cgcctctgca
     1861 gactccgcat ctcccaaaac cccaggctgc aaacgactta cggcatccat gagcaccagg
     1921 gcggccggtt cccctcccga caggacgtaa tcgccgatgg agaactcctc ggccccgagc
     1981 gtctcgacca cccgctcgtc gatccccttg tagcggccgc acaccaccgc gaggtgcttt
     2041 tcggcggcca gctcccgcac ccacacctgc gtgagcacgc gtccctgggg cgaggtgacc
     2101 agcgtccgag gagccgcacc cccgcacgag cgcgcgacgc tctcgtgggc agcgacgatc
     2161 ggctcggcct tcatcaccat gcccgccccg ccgccgtagg gatagtcgtc ggtcgtgccg
     2221 tagcggtcgt ccgcgaacgc ccggatcggc acgagcacga cctcgaggag ccccttctcg
     2281 cgggccacgc ggatcatccc ctcgtccagc gccccgccga agaaaccggg gttcagcgtg
     2341 aggatcgaaa cgcgcacggg gggttactcc cccccaatcc ccggcaatcc cggaggcggc
     2401 tcgacgacga gcgacttccc ctcgaggtcc acggagacga tgaccccttc gagcatcgga
     2461 atcaggatct cgttctcgtc gtcgtccacc accagcacgt cgttgctacc ggtgctgaag
     2521 atgtcggtga cgcggcccag gggctcgccg ccggtcgtcc gaaccgtgag accgaccagc
     2581 tcgaactggt agtgctttcc ttccggaaga ggcgtcacct ggctgcgcgc gatcgtcagc
     2641 tcctggccgt ggatcgtctc cgcctcctcg cggctctcga tcccctcgaa ccgcacgagg
     2701 agcctcccct ggaaggggcg cgacgtcgcg acgcggagcg gccgcgcgtg caggctctcc
     2761 ggcgcggcgg cggtcatctc gctgccgggg gcgaagcgct cgggcgcgtc gctcatgact
     2821 ttgacgacta cctcgccgcg gagtccgtgg gctttggcga cgatcccgac gaggacgcgc
     2881 gcagcgtcac cgacttcatc aacccctggg ttcattggct gctgggagtt ccggccgcct
     2941 gtgggcggcc gggatattaa tccgcaattt ccagaatcgc ccggcgcccc tgggccgtga
     3001 ccgacgcggt caacagcgcg cgcagcgcgt gcgccgtgcg gccctcgcgt ccgatcacct
     3061 tgcccagatc gcccggcgag acgcggagcg tgagcaccgt ggtctccccg gtgtcgtgct
     3121 cgtcgacgtg aacgccttcg gggtggtcca cgagctgccc cgcgagatat tcgatgagcg
     3181 ccttggcctt catgtcgcct ccctggcggc gccgtggcgc cgccggtcgc tcagctcttc
     3241 ttcttcttgg ccttcttgac cgcgcgcatc ggcgtaacac cgctcttctt cttcgcgcgg
     3301 gccttggcct tcttcgccac gcccttcgtg gcggccttct tcggcgcctt cttcttcgcg
     3361 gcgcccttct tcttcgcggc cttcttcggc ttcgcggctt cggcctcggc cggagccggc
     3421 atcgcggccg cggtctgcga cacgagcggc gcggggcgga gcgcctcggg agcgtgcttg
     3481 gccatcatca cgccgaccga ctccgacgga gtcgcgccct tgcggatcca ggccgcgaac
     3541 ttctccgcgt cgaccttgaa cgtcgcgggg tcggtgagcg gatcgtagta gccgagctgc
     3601 tcgatgtagc gcccgtcgcg ccggctcctc gagtcggcga ccacgacccg atagaacggg
     3661 cgcttcttcg cgcccgcgcg tttcatgcgg atgacgacca ttctcttcta ccctccgtcg
     3721 gtgttcggcc cgggccgtac ggcctcagcg gcgcggcatc cccatcggga accggccctt
     3781 cggcatcttg cccatgcgct tgagcatcgt ctgcatctct tcgaactgct tgaggagccg
     3841 gttcacgtcc tgcacggtcg tgccgctccc cttggcgatc ctccggcgcc tgctgccgtc
     3901 gatgacccgc ggctcttcgc gttcgccccg cgtcatcgac tggatgatcg cctccgtgcg
     3961 cttcagctcc cgctcgtcga cctgcgcgcc ggcggggagc ttcatgcccg gaatcatctt
     4021 catgatctcc tcgagcggac ccagcttctt catctcctgg agctgcccga ggaaatcctc
     4081 cagggtgaac ttctgcttcc gaagcttctt ctctaggtct tcggctttct tgagatcgat
     4141 gcgctcctga acgcgctcga ccagcgtgac gacgtcgccc atgcccagga tgcgcgaggc
     4201 gagccggtcg ggatgcagcg gctcgatgcc gtccagcttc tcgccggtcc ccaggtaccg
     4261 gatcggcttc cccgtcacgg accggagcga gagcgcggcc ccgccgcgcg cgtcgccgtc
     4321 catcttggtc aggacgaatc ccgtgatcgg aagccgcgcg gtgaacgact ccgcgatcga
     4381 caccgcgtcc tgaccggtca tgccgtccag gacgaggtag ctctcctgag gctcgagctt
     4441 cttgtggacc cgctcgacct cggtcatcag ctcctcgtcc acatggagcc ggccggccgt
     4501 gtcggcgatc aggaggtcga agccttcctt gtccgcgcgg cgccgcgcct cgtgcgcgag
     4561 ggccaccgga tccttcgccg cgcggtccag gtgcacgggc gccccgacct gcttccccag
     4621 cacctcgagc tgatcgacgg ccgcggggcg ctggagatcg agcgccgcga gcagcacgcg
     4681 cttctcgcgc ttcatccaga agcgcgcgag cttcgccgcg gtggtcgtct tcccggagcc
     4741 ctggagtccg gcgaggagca gcacggccgc gcgattggcc gggaactgga gccccgtctg
     4801 cgggcccgcg ccgccgagga gcttcaccag ctcttcgtgg acgatcttca cgacctgctg
     4861 ccccggctgc acgctctgga gcaggtcgga gcccaccgcc cgcgcctcga ccgacttcag
     4921 gaagtcgcgc gcgacgttca ggttgacgtc cgcctcgagc aggacgcggc ggatctcgcg
     4981 gagcgaatcc ttgacgtcct gatcgctcaa gcggccccgg cccagcatct tccggaaaac
     5041 gccgcccagg cgttcggtca gatcttcgaa catggcgctc gcagagaacg ggtacggcgg
     5101 cgcggccaga cgccgcggtc ggcgaggcca agtgccacaa acgggagaag ataccattgc
     5161 ggccgcgcac ccgcaactga ggaagggggc acgagtggta tgccccgcgc cccccagaaa
     5221 ccgtggttcg accgccgcaa gctcagcgca cgatggccgc cttggtcgtc gacttcccgt
     5281 ccggggtctc gatccggaac agatagatcc ccgagggcag cggcttgccg gagtcgccac
     5341 gtccgtcgaa cgccagcgcg tgctcgccgg cgaacacctc gcggctagcg ctcaacgtcc
     5401 gcaccagccg gccccggatg tcgaacacgc gcgcccgaac cggcccgggt cgtgacgtgg
     5461 agaagaggat cgaggcaccc gggttcggcg gattgggcag cagcgccacg gcgttccggc
     5521 cacccgcgat cacgtccacg ctcagcgcgg cttcgaagcg attcccgccc gtcaggtcgc
     5581 cccggatcgt gacggtcacc gtgctctggc ccgtgagatt gctgaacagc tgccggagat
     5641 cggtcttggc gaaacagacc tcgaagtcgg ccaccgtgtt ccggttcgcg tcgcccgcga
     5701 cgatgccctt cccggcgatc gcggggatct gggacaccga tccggttcca ttcgagatca
     5761 gaacgatgga cgtcggcacg acgtcggtga cggcgaagtc tccggataca ggctccacgt
     5821 tcgcgcacca tgcggctttc cccgacccga gacggatcgt cccgttaccc ccggtcgtga
     5881 acgcggtcgc ggccaggagg tcgcccgtcg cgttgaccgc cgtcgtcgcc gaacctgtga
     5941 gtccatggga cgtcgccacg aagagcacga agtaggtccc gccctgtccc gcggagggcg
     6001 accagtcgaa agctcccacg gtgtttgcga gattcggtgt gaacgtcgcc ccttgaggca
     6061 ggttcgaagc ggtcagcgac tcgatcggcg tgtcgtcggg atcggcggcg gagacggtga
     6121 actggatcgg tgtcccgacc gcgcccgacg cggcggccgg agccgtgacc accggcgccc
     6181 ggtcacccga cttgatcgtg atcgggagcg tggcgcttcc gctcagggta ttggacgcga
     6241 tgaaggtcac gttgtagatc cccgcctgcc ccggagaggg cgtccagttc aaggttcccg
     6301 acgtattcga cgcactggag gtgaacgtgg ctccggtggg cagcggcgaa gccgtcaggg
     6361 agatgatggc atcgccgtcg ggatccgtgg cggcggcaca gaccgagaac gggacggttt
     6421 ccgagcccgt ggcgctcgga ggcacgttga ccacgggagc gcgatcgacc gacgagaccg
     6481 tgatggaggt gttgtaggtg gcggacgcgg agctggtcgc cgtgaacccg acgttgtagg
     6541 agccggcctg cgtgaaatcg ggagtccagt cgaacgtccc cgccgtgttc gtggcgttca
     6601 ccgtgaaggt ggcatggttg ggaagcggcg ccgccgtcag cgagacgatc ggctcgccat
     6661 ccggatcccc ggccgtaacg tggaaggaga gcggtcggaa ctcccccgtc gtcgcggtgg
     6721 agggacactg cacgaacggc gcgccagccg tgtcggtcac gttgatggtt gccgatgcgg
     6781 tggccgacaa cgcgttgctc gcggtgaacg aaacggagaa cgaaccggtc tgcccggcct
     6841 gaggggtcca ggtgagcgtc ccggccgtgt tcgaggcatt tgtagtgaac acggcgttat
     6901 tgcctgccgg cagcgccgat gtattggcgg tcagggagag aatcccgtcg ccattcggat
     6961 cgagcaccgt gacctcgacg acggcctgga caccgacggc cgcacggatt ccgctcgtgc
     7021 tcactcgcgg ggcgaagtcg cagaagctct gtgccgatac cggaggggag tgcagcgcga
     7081 aactgaagag gagcaatgcg agaacgccga gaggggagcg accggggcgc atgacgacct
     7141 ccactcgcgc cgccggggac ggcacgggta cgtgaaatca ggccgttccc ggaacctaac
     7201 atatccaata aggaatgttt acaggagatt cgcgagcctc gacccgccgc ggattgtcgc
     7261 gaaacgttta cagcccattt cctcgccttg gaggccgatt tgtgcgtagg tatacgaaag
     7321 gggttgacat gaatttcgag tgtgagataa cttttctgtg agcggagttc cctgccgctt
     7381 cgtgcccaat ccgcggctaa aacctagtcc atcaggccgg tcgtttcttg cggggtttta
     7441 actggcgaga cacgtggatg cgcggggaac ttcctcaaat tttttttgcg ttcgactccc
     7501 ctcctctgcg attctgacgt cgcggtccca gtcaccgttc cccagacatc gtcccccgcg
     7561 gccggaatag gagcaccgca ctgcgccagt ggctcgccgt tgccgcgatc gcgctcgtcg
     7621 gttgcagcgc ggacggcatc cattccaccc agaaattctt ccccggctcc cagcgtacct
     7681 cctgggatga gtgttccaag ttcgggtcga cttcgaacac ggtcgcgatc tgcgcgcaga
     7741 ccggactcct ccccggcgcc ccggccccct cgtacaacat cgatttcttc attccgaccg
     7801 cggaacacgt gctgatcgcc gtcttcgacg agcgcgccac gcgggtgaaa gtgatcttcg
     7861 acgccgacga gcccgcgacg ctctcgggaa ccttccggct gccggtcatc ccctgggact
     7921 tcaccgacgc cgagggccac cgcgtcccgt ccgggaccta ccggctctac ttcaaggcgg
     7981 ggagctttat ctcgaccagc gacgtggacg tcccatgagg cggcttctcg cggcagcgtc
     8041 ggcgctggcc tgtttcgcct gggccggcgg cgctcccgct cagaccgcgt cgccggacac
     8101 cacggcgcag gcacctcccg gcggagcgac ggtcccgagc gtttcctccg acacgaccgc
     8161 ggcgccggtc tcggccgccc ccgcggacac gaccactccc tcgccggtca cggccgcccg
     8221 cgccgcgcgc gtggccgtcg gcaaggacaa cgaccggttc gagctgggcg ccgcggtcgc
     8281 ggacggaccc ttcgacctcc tcggcacgtt cgggtacgag cgctacgtcc agcggggcgg
     8341 gcccttcgag cactggatcc acgtcgaggt gtcgggaggc gccaccccgt tcctgaacga
     8401 ggggacgctc agcgtcgcct acctgctgcg cccgctcggg acgatccgcc gcacgtgggc
     8461 gatccggccg atcctggagg ccggaccggg cgcgcacctg gtcgttcagg tggcggagat
     8521 ccaggggttc ggagagaccg cgttccactc gcgcgtgtac gtgaagacgc acgccatcgc
     8581 ggggctcgag gcgctgctcg gccggcgctg gggggtggtc gtgcgcggcc gcttcagcgt
     8641 cccggcgcac caccccttcg actacgcaca gatcgcgctc ttccggagat aggcccccgt
     8701 ggccgagcgc tcgtcgcgcg cgatcgcggc gggcggcccc tcggccgtga tcctcccgcc
     8761 ctcctcgccc gcgcccggac ccagctcgac cagccagtcg caggcggcca ggaagtccag
     8821 gttgtgctcc accgcgatca ccgtcccgcc gcgccgcacc aactcgcgca gcacgcgaag
     8881 cagcgtctgc acgtccttcc ggtggagtcc caccgtcggc tcgtcgaaga gatagagcgt
     8941 ctcccccgcc gcccgatccg acagctcgcg cgccagcttc acgcgctgcg tctcgccgcc
     9001 gctcagggtg gagaagggct gccccagccg caggtagccc agcccgaccc gctccagcac
     9061 ccagaggggc gccgccacgt cgggatcgtc gcggaagagc tggatcgcct cctccaccgt
     9121 cttgtccagg agcgcgtcga tcgagagccc gtgcacggtc acctcgagca cctcggggcg
     9181 gaagcggcgc ccctcgcagg cctcgcaggg gatccagagg tcggcgagga agtacatctc
     9241 gacgcgcgtc acgccggctc cttcgcaagc ctcgcagcgc ccgcccgcga cgttgaagga
     9301 gaaggtcccc ggggtgtact tccgctggcg cgcgagcggc gtccgggcat agcgctcccg
     9361 gatccggtcg aacgccttca cgtaggtcgc gggattggag cgcgcgctcc gcgccacggg
     9421 cgactggtcc accatggcca cgcgccggaa gggctgcagc ccgagcaccg cgtcgtgcgc
     9481 gccgggagcg ggcgcgtcct ggtcgagcgc gcgggccgcg gcccgccaga gcacgtcctc
     9541 gatcagcgtg gacttccccg acccggagac gccgcacacg ccgacgagcg ccccgaccgg
     9601 aaaccgcgcg ctcacgtcgc gaaggttgtg ctcgcgagcg cccctgactt cgatccaggt
     9661 ccctttcggc ttcttcccct tggccgcggt cccggcctcc ccatacgagg cgcgcccctc
     9721 ggccgccatc tcgggcggct cggtcgcctc ggcccgtgaa ggccacgcct gcaccggtcc
     9781 ggcgtagagc agagcgccgc cgcgctgccc cgcgcccggt cccagctcca cgatccagtc
     9841 ggccgctccg atcacgtcgg ggtcgtgctc caccacgatc accgtgttgc tccgctcccg
     9901 gaggcggcgg atcacgcgga tcagtctctc cgtgtcgcgc ggatggaggc cgaccgtcgg
     9961 ctcgtccagg acgtagaggg cgtgcgagag cgatcccccg agcgcgttcg ccagctcgat
    10021 ccgctgcgac tcgccgcccg aaagggtctt ggacgcgcga tccagcgtca ggtagtcgag
    10081 cccgacttcg ccgagatact ccagccggcc ctcgacctcg gtccggatcg aggcggccac
    10141 cgcctcgcgc acgggatcga ggcggagccc ggcgaagaag gcgcgcagct cgccgacggt
    10201 ggcgccgagg agctgggcga cgttcttccc gccgacgtgc acctccatcg cctcgggctt
    10261 caaccgcgtt ccgccgcaga gcgcgcaggg catcgcctcc tggtagtgct ttaccaggaa
    10321 ccgcgcgctc gatttgtacg acttgagctg gaggcgctcc aggaagggga acacgccccg
    10381 gaaccccggc gcgccgtgga gaaggacacg ctgctgctcc tccgggaggc tcgcgaacgg
    10441 aacgtccagg gagatgccgc gctccttgga gagcgcctcc agcttgggca tgaagtaggc
    10501 gcgccaggag ttcttccacg gatcgagcgc cccctcgcgc accgtcttct ccggatgcgg
    10561 caccacgcgc tccagggtga acgtgagcac gtttccgaag ccgcggcact cggggcaggc
    10621 gccgtagggg ctgttgaagg agaagagctg gggccgggga tcggggaacg tgcggccgca
    10681 cgtgacgcag gtccggccgt cggcaaaggt gaggacctcg cccgagggcc gctcgatatc
    10741 gagccgcccc tccccctcgc gccaggcaag ctcgatcgcc tcgcgcaggc gcatccggtt
    10801 ctcggcgccg gcccgcacgc ggtcgatcat gacccggatc ggccccttcg aaccggccga
    10861 aagaggctcg tccagccgaa gccagctctt ggcgccgcgc ggctgcgcgc gcacgaagcc
    10921 cgcggcgagc gctcgcgccc agatctgggg cggccgctct cccggcctcg ccggaagggc
    10981 cgcggccacg atcagatcct cgccctcgaa ccggagcgcc gcctccagca ccgaatccac
    11041 gctgtagcgc gggaccaccg tcccgcactg gccgcagcgc gtctcgccga cgcgggcgag
    11101 caggagccgg aggtagtcgg cgatctcggt gacggtgccc accgtggacc ggccggtctg
    11161 cgcggccgcc ccctggcgga tcgcgaccgc cggcgagagc ccctcgatcc ggtcgatctc
    11221 gggccggggg agccggtcga ggaactgctt cgcgtacgtg gagacgcact cgacgtagcg
    11281 ccgctgcccc tcggcgtaga tcgtatcgaa ggccagggac gacttccccg aaccgctcac
    11341 ccccgtgatc acggagaggg ctgcgtgcgg aaaggagacg tcaattccct tgaggttgtt
    11401 ctgccgggct ccgaagagcc ggagatgggt gtcgggagca acgaattcgc ggcggggcac
    11461 gggcccattg taatgcacga gggccgcccg gcattgccga gcggcccctc aggacgtgcc
    11521 caatccgcta acttacttct tcttggcggt cttcttcttc gcgctcttct tcttggccgg
    11581 catcaggatt cacctcctcc caggttggat tcgggttccc ggctcatccc ggcgtcgagt
    11641 tcccgccggg tccgatcagg cttcgcactt catccaccag cgcggtcagg tcgaacggct
    11701 tcttgaggta ccggtccacc ttgaggcgcc gggcttcggt cttcacctcg ggcgtctcgt
    11761 acgcggtcat gagaatcact ttcgatcgca gggaccgttc acggagccgc ttcaccatct
    11821 cgatgccgct ctcgcgcggg agccggacat ccaccacgat cagatccatc ggcgactcgc
    11881 cgaagacgtg gcgctcggct tcctccgccg atcccgctac gacggttcgg taccctgctt
    11941 cacgaagcac ggtggagagg gtgcgcgcca agagttgttc gtcgtccacg atgaggacgg
    12001 acttgtgcaa tccacacctc cccctctctg cgctcgtgtc atcgctatta attgattgca
    12061 ggaagcaggc caagttgagt gcgcgagcgc gcggagcgcg cgaagtgaat gcatcgcgcg
    12121 tggcgcgcga cggttagacg ttcaccttca tgcgtgacgc gagcgatgcg cggcgcttcg
    12181 cgcgatcgcg cgactgtcgg ggaaatggac agctgaacgc gcgcgcgagg cgatctacga
    12241 gggaaaatgc acgcgctgtc gtgaaaggcg acatcccaag aaaggctaca gatgtacttc
    12301 gcgatcgcaa agcgatcgcg aagcgggagg ggcggggaag gggtttttct cagcgttcag
    12361 cggaaaaaaa tctcaggtcg agctcaacga ccctcgcgtc gcggcgatcc gtttcaatct
    12421 ttcgatcaag ctctggcggg aaatgccgag aatacgtgcg gcgtgcgagc ggttgccctg
    12481 cgtggactcg agtaccttga ggatgtgctc ctgctccact tcggcgagcg tcggcatctc
    12541 ggcgtcgcct tcgccacgcg cggcgagcgc ttcgcgaagc acgcgcggca gcgattccac
    12601 ctgcgccatc atctccgcgg gcaggtggtc ctcgcgcagc gcctcgtcgt tctccaagag
    12661 cacgatccgc tcgagaaggt tcttcagctc ccggacgttc cccggccagc gataggcgcg
    12721 gaggatcccc tcgacctcag gcgtgatgga aacgaagctc ttccggaact tctcgttgta
    12781 gaaggagagg aagtgccgcg tcaggagcag gacgtcgtcg ccccgctcgc gaagcggcgg
    12841 gagcaggatc gacacgacct tgagccggta gtagaggtcc tgccggaact ttcccgcctg
    12901 gacgagctgc tcgagatcgc ggttcgtggc cgcgatgagg cgcaggtcca cggcgaggtc
    12961 ggtggaaccg ccgacccgct tgaaggtgcg gtcctcgatg aagcggagga acttggcctg
    13021 cgtcgccgca ggcaggtccc ccacctcgtc caagaagagc gtcccctcgt ccgccagctc
    13081 caccaagccg cgcttcggct cccgcgcgtc ggtgtaggcc ccgcgctcgt ggccgaacag
    13141 ctcgttctcc aggagctgct cctggaacga ggagcagttc aaagcgagga agggggcgtc
    13201 gcgccgtccg ctctcgaagt ggatggcccg cgcgaccagc tccttcccgg tgccgctctc
    13261 cccttcgatc agcaccgtgg tgctctcgct ctgcgcgacc ttccccgtca gatcccaaat
    13321 ccggcccatg gcgggcgact cgctcacgat ccgggtcttc aggaaggtct cgagctcgcc
    13381 gcggcggaag gcgtcgagct gggtctcgcg gacgaccccc gccttcaggc gctccatcgc
    13441 caggagcacc tcttccattt cgtacggctt cttcaggaag tcggtggcgc cccggcgcat
    13501 cgcctccacc gcggtcggca ggtcgccgaa cgcggtgatg accaccacct tgaggtcggg
    13561 cgcgagcgac ttgaggcgcg gcaggaggtc gaggccgctc ccgtcgggaa ggcggaggtc
    13621 cagcaccagc acgtggggcg ggttccggcg gaccgaatcg agcacctcgg ccgcggtcgc
    13681 gaccgagtcc acctggtagc cctcggccgc cagcagcttg gtgagcgact ttcggatcag
    13741 ctcttcgtcg tcggcgatcc ggacccgaag gatcacgtca ggccttctcc tctttcgctt
    13801 cttcggcggc ggccggctcg gccggcggca gcggcaccgg caggctgaat cggaacgtcg
    13861 cgccctgggt caggttccgc gcggtcaggt ttccgccgtg ctcgcgcgcg atctggagcg
    13921 agatcgagag cccgagcccg gtccccatgg agcgggtcgt gaagaaggga tcgaagatct
    13981 tctccttcac gtcctccggg atgcccggcc ccgagtcggt gatcgacacg tccacgtacg
    14041 ccttcttgta gcgcgtccgc cggagtccga tgctcgcccg gagcgttccc ccttcgggca
    14101 tcgcctggat ggcgttgatg accaggttca ggacgacctg ctgcgccatg tcgggatcca
    14161 cgaagacctg gggcaccggc gggtcggggt ccagctcgcg ctccacttcg accgaggcgt
    14221 cctcgaggcg ccgcgccagg ttgtcgagca ccttctccac cacctcgcgc aggtcggtcg
    14281 ccaccgggcg cgccgcctgc ggccgggcga acaggaggag gtcggtgatg atctgctcga
    14341 tccggtccag ctccttcagc acgtcgtgca ggtcgtcgcg gcgggaatct cccgcgcgga
    14401 gcttggatgc gacgaactgg accgtggtgc gcacgccggt gagggggttc cggatctcgt
    14461 gcgcgacgcg ggcggagagc tcgcccaggg agatgaggcg ctccgtgttc cggaagacct
    14521 cctcttcggt gtcggcgtcg accaggtcct cgacgcggat caggagggcg ccgctgtccc
    14581 cgagcggggt cgaggtgtag cgcatcagca cctcgcgatc ggcgacggcg ccgcgggtct
    14641 cgcgcgtggc gcggccctcg gcgcgggcgc cgcgctcggg cagataccac cgttccgtct
    14701 cggggtggaa gacttcggag aggggcttcc cggtcacctc atcgcggtcc acgccccacc
    14761 cgcgggccag cgtctcgttc aaggcgacga tgcggcggtc ctcgtcgagg gccaggagtc
    14821 cggtgtcgag ggcgtcgagc gcctggagga acagctcgcg ctcgatctca ggtgtttcca
    14881 accgcgccat tccgcccccc cgcggtgacc atcgaaacca ttgaagtcac gttgcctcgc
    14941 ggcccatcgg ccgtcaaccc aaatcgagcg cgcttgcatg cattcgcgcc ggttgctagg
    15001 cttttacccc gattcccggc atgaccgcgg cgccgccagg gcgccgtccc tgtttccacc
    15061 cctgatccgg atcgaggttt cacgccatga tgcagtccct tcgcgacaat atgaagctga
    15121 tcatctggat cacggccatc gtgttcctgg tcggcttcgg catcctccag ctcggcggcg
    15181 tcttcggcaa ccagccgacg cagcagcgcg ggcccaccgg catcgtcgcc gacatcaacg
    15241 gcgagccgat ccgatacgac gagtacatgg gcacggtgaa ccagatgacg cgccagctcg
    15301 agcagacccg ccccctccag gcgggcgagg acgcctacat tcgcgagcag gcgtggcaga
    15361 ccctggtccg gaacaagctg ctccagcagg aggcgaagcg gcggcacctg ggcgcgacgc
    15421 ccgacgagat caagatggcg ctccgcgtgg caccccccga gttcctggtg caggccgatg
    15481 cgttcaaggg ggcgaacggg cagttcgact accggaagta cctcgccgag ctggacaacc
    15541 cgaactcgca gatgccgtgg ggccaggtgg aggcgctggt cgccgatcag ctcccgatcc
    15601 agaagctcca agaacagatc gtggccggcg ccaaggtctc cgagggcgac gtccgggagc
    15661 gtttcctgct ccagaacgac aaggtgaagt tcaaggcgat ccagttcctc cccgacagct
    15721 tcccggtcga tacgtcgcgc atcggcggcg ccgacgtcga gtcctactac aaagcgcacc
    15781 ccgacgagtt caccggcccc gaagaggtga aggtcgcggt ggtcctggtc ccccggaaac
    15841 ccgacgcgac ggacttctcc acggagaagg agcggctccg cgcggtgctc gacatggcgc
    15901 gggcgcagcc cgactccttc ccctcgctcg cgcgctcgta ctccgagatc cagagcgcca
    15961 gccgcggcgg cgatccgagc tcggagccct tcttcgatga gcttcggccg atcttccgcc
    16021 gcggactcca gaacctccag gccggacaga tcaccgacat cctccaagaa gagcgctccc
    16081 tacacatctt caaggtcgag aagcgctggg tcgatcccgc caccaagcgg gagaagatca
    16141 agtatcgcga gatcgcggtc cgcgtcgagc cgggcacgaa cgcggtgcgc gcggcgcgcg
    16201 agctcgccca gaaggccgaa aaggacgcga agcgctccgg tctggaagcg gtcgcgaccc
    16261 gcatggggac gcggaccttc acgtccgagt acttcgcgta cgggcagagc ggcaacgacg
    16321 tgctccagcg cttccccgag gtcgagacct ggatgttccg ctccaaggcc ggcgccgtct
    16381 cggagatcgt gccgaccgag aacggctact tcatcttcca ggtggtggat caccgccccg
    16441 ccgggctgcg cccgctggac cgcgtggcgg cggacgccaa ggcgaagctg gtccgctcgc
    16501 tccgcatgca gcgcgcgaag gaggcggcgg accaggcccg cgccgccctg gccggcggcg
    16561 ccgccgagcc cgaagtcgcc gcccgcttcc acggccgcgt cgccgacgtg cctgaggcca
    16621 cgcggaacgg ctacctgtcg acggtaggcg ggcgcgagcc ccgcgtgatc ggcgcggtct
    16681 tcgcgatgcc ggcgggcgcg ccctggtcgg cccccctcgt gggcgacaac ggggtctacg
    16741 tggcgcaggt cgaggcgcac atggcgccca gcgaggacga cttccgcaaa caggaggcgc
    16801 aggttcggga ccagctcctg aacgagcgcc ggcagctgat cttcgtggag tggatgcagg
    16861 acctccgccg caaggcgaag atcaaggact accgcgagca gtatttcgag gtttgaggat
    16921 gtcgggggcg gcggagccgg acgctccgcc gctcccggcc ctgcgttacc ggttcgatcc
    16981 ccgcttcgag cttcctcccc gctccgcggt cgcgcccgcg cccgtcccgg cttccatcgc
    17041 ctcgacgttg atcgtgcgct ccgcgatctg ggtcagcagc tcgtcggccc gcttctcctc
    17101 gtccagggtc tcttccagga gccgcgcggc cttctgatta cccagcaggc ccgcgtaggt
    17161 cttgaggcat ccgtaggcgg ccatctcgta gtgctccacc ctctgcgccg cggaaatcag
    17221 gccggcgtcg aggacctcct cctccacgtc ctcgctcaga agctccttcg cctcctcgat
    17281 caaaccttcc atccccatgc acttctttcc cgtggggctc ttgccgaggt ccttgaagat
    17341 ctggtccagg cggtccacct gcttctcggt ttcgcgcagg tggttgtcga aggccttcct
    17401 gagctcgtca ttgcttgcgg ccttggacat gcgcggaagc gcccgcagga tctgcttctc
    17461 ggcgctgtag acgtccttga gctcatccac gaatagtttt tccaggctgt cgatctgcat
    17521 ggttcacctc tccgccgtca gtgtttgcgt gttgcgtttc cagtacgact cgggcggcga
    17581 ggtgcaacac gtatgccacg gttttccgcg gccaggaccc gctcttccgc ctgcagctcc
    17641 cggttgatcg ccacggcggc cttggctccc tcggccgcgg cgaccaccac gaactgcacg
    17701 tcccacgagg cgtcgcccgc cacgtagacc ccaggcacgt tcgtccgctc gcggcggttc
    17761 gtgcggatcg ccgccttgag ggtcagccgg cagcccagcc ggatcgccag atcgcagctc
    17821 tgatcgttcc cggtggaaaa gaagagcgcg tcgcgctcca gaggggcgcg cccgcgcagg
    17881 agcacgcggc gg
//
LOCUS       DASSLZ010000300         5075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1109686, whole genome shotgun sequence.
ACCESSION   DASSLZ010000300 DASSLZ010000000
VERSION     DASSLZ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109686"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VFM00_11405"
     CDS             complement(<1..343)
                     /locus_tag="VFM00_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498896.1"
                     /translation="MNRILAILAAALVLGASAHALAADPPAKSAPDKAKAAAKAEPTR
                     AAVVSKKSLQMGKPPAELQKLAVLLGSWNGETHAFAGADAKSPSKSTYKWTFNGMHLE
                     GDHQYTMNGKPF"
     gene            438..1397
                     /locus_tag="VFM00_11410"
     CDS             438..1397
                     /locus_tag="VFM00_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7498897.1"
                     /translation="MNDARSRHGSPQDASSGEPAIVASDLVKRYDEVHAVRGVSFEVA
                     PGEVVGFLGPNGAGKTTTVRMISCFLPPTSGTARVFGVDVRERPRDVKRMLGICPQED
                     NLDPDFDVLKNLLVYGRYFGIPGAEVKRRALPLLEMMALQDKAKSPVASLSGGMKRRL
                     ILARALLNEPRVLVLDEPTTGLDPQARHAIWTRVRALRATGVTVLLTTHYMEEAAQLC
                     DRVVIIDEGKILLEGTPEELVRREVGKSVIEIWNHGPDVVLSMRQGPGRLEEVGDRLY
                     LYPEDEEQAVHLLESRFPGQDRLLRRATLEDVFLRRTGRALRD"
     gene            1400..2188
                     /locus_tag="VFM00_11415"
     CDS             1400..2188
                     /locus_tag="VFM00_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7498898.1"
                     /translation="MREILRNLSLRSLRVLQRDADVYFTVWKTEFLPPLLEPIIYVFG
                     FGLGLGQLVHDVSYQGRTLSYLAFMAPGVLAVAIMFWAFFETTFSSYVRMYYQKTFDA
                     ILATPLLVEDVILGELLWGTVKSMIASTIMLGVLSALGLVHWPTGLWVVPIAAAGGLL
                     FSGFGLIMTSAVKTISQFNVPMFAFVLPMFTFAGTFFPLDILPKWALAVAWCLPLTHI
                     TILVRSAVLGMGHPMLPVSVAYVLIVGLGLGAWSLVRMKKRLIQ"
     gene            complement(2451..3689)
                     /locus_tag="VFM00_11420"
     CDS             complement(2451..3689)
                     /locus_tag="VFM00_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7498899.1"
                     /translation="MSPSLPDPHHPTSEELFAYRDGELTADRRTLIEAHVLACAQCSE
                     RLEEMSAAEAELRLRPDGAGEEYFERMTESVMAKIGSPGREAVIGVTTRETAASAPGA
                     PEPRTHARRGDYVDVPRVERRRPDSSSEELEPRRRLHLPWIGIGGGVVAAAAVVVVAV
                     VLFQRQSEWVRAPRPAVLGEPGIEPRADSLQTERQESSNGVAGAPSGARSREVAPSRE
                     KTAAKKSDRDRLLAKSEAKALPPSAGAVSGAGDVSLKDQAPEIRTQVAPQALRVPGTE
                     GDRKLMASQNAAPEAAPRGAAVPGDAYTNVLRAYRLPPVWNSGVPGNAVARAETDLRY
                     LYQTGRAGADSARIRLYLAEAERARLTDPSNTAAVESISHHYRRAISLAGGDATLAAT
                     ARRRLAEFEQEMTRARGAQP"
     gene            complement(3686..4282)
                     /locus_tag="VFM00_11425"
     CDS             complement(3686..4282)
                     /locus_tag="VFM00_11425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7498900.1"
                     /translation="MAPARPDRSSLSDGELVRRIRDGEADAYAELVRRSRRSGYRLAR
                     RITRNHEDADDVLQESYVKAFRALDRFAADRPFGPWFLTIVARTALTWIRDAKRRAAE
                     PLDEPGVDGSAPLADRVADPAQDAAALERRVQVERAYAKLSEDHRMILALRVDADLSY
                     AAIAEALAVPVGTVMSRLARARESLIEALHELREAEPR"
BASE COUNT          740 a         1719 c         1741 g          875 t
ORIGIN      
        1 agaagggctt tccgttcatc gtgtactggt ggtcgccttc gaggtgcatg ccgttgaagg
       61 tccacttgta cgtggacttg ctgggactct tcgcgtccgc gccggcgaac gcgtgcgtct
      121 cgccgttcca ggacccgagc agcaccgcca gcttctgcag ctcggccggc ggcttcccca
      181 tctggagact cttcttcgag accaccgcgg cgcgcgtggg ctccgccttg gcggcggctt
      241 tggctttgtc cggcgcggat ttggccggcg ggtcggcggc gagagcgtgg gcggaagcgc
      301 ccagcacgag ggctgcagcg aggatggcaa ggatgcggtt catggtgctc ctcctggatc
      361 tggggtgagg ttgtaatgcg aagggaaaag gctaccgtgg cctcccgccc cacgcaagat
      421 ggtagagtag ccggcccatg aacgacgcac gctcccgcca tggctcgccg caggacgcct
      481 cgtccggcga acccgccatc gtcgcgagcg atctcgtgaa gcgctacgac gaggtccacg
      541 ccgtccgcgg cgtctcgttc gaggtcgctc cgggggaggt cgtgggcttt ctcgggccca
      601 acggcgcggg caagacgacg acggtgcgga tgatctcctg cttcctgccg ccgacctcgg
      661 gaaccgcgcg cgtcttcggc gtggacgtgc gggagcggcc gcgcgacgtg aagcggatgc
      721 tcgggatctg cccgcaggaa gacaatctcg accccgattt cgacgtcctg aagaacctcc
      781 tggtctacgg gcgctacttc gggattcccg gcgccgaggt caagcggcgc gcgctgccgc
      841 tgctcgagat gatggcgctg caggacaagg cgaaatcacc tgtcgcctcg ctctccgggg
      901 ggatgaagcg gaggctgatc ctcgcgcgcg cgctcctgaa cgagccgcgc gtgctggtgc
      961 tggacgagcc caccaccggg ctcgatccgc aggcgcggca cgccatatgg acccgcgtgc
     1021 gcgcgctccg cgcgaccggc gtgaccgtgc tcctgacgac gcactacatg gaagaggcgg
     1081 cgcagctctg cgaccgggtc gtcatcatcg acgaggggaa gatcctgctc gaaggcactc
     1141 ccgaggagct ggtccggcgc gaggtcggaa agagcgtgat cgagatctgg aaccacggcc
     1201 cggacgtggt cctgtcgatg cgccagggtc ccggccgcct ggaagaggtc ggcgaccggc
     1261 tctatctgta ccccgaggac gaggaacagg cggtgcatct cttggagtcg cgtttccccg
     1321 gccaggaccg gctccttcgg cgggccacgc tggaggacgt gttcctgcgg cgcaccgggc
     1381 gggcgctccg cgactgacca tgcgcgagat cctgcgaaac ctctcgctcc gttccctgcg
     1441 ggtcctgcag cgcgacgccg acgtctactt cacggtctgg aagaccgagt tcctgccgcc
     1501 cctgctcgag ccgatcatct acgtgttcgg gttcggtctc ggactggggc agctggtgca
     1561 cgacgtgagc taccagggcc gcacgctctc ctacctggcg ttcatggctc ccggggtgct
     1621 ggcggtggcc atcatgttct gggccttctt cgagaccacg ttctcgtcgt acgtccggat
     1681 gtactaccag aagacgttcg acgcgatcct cgcgacaccc ctcctggtcg aggacgtcat
     1741 cctgggcgag ctcctctggg gcacggtgaa gtcgatgatc gcgagcacca tcatgctcgg
     1801 cgtgctctcg gcgctggggc tggtgcactg gcccacgggg ctgtgggtcg tcccgatcgc
     1861 ggccgcgggc ggactcttgt tttcgggctt cgggctgatc atgacgtccg cggtgaagac
     1921 gatctcgcag ttcaacgtgc cgatgttcgc cttcgtcctc ccgatgttca ccttcgcggg
     1981 gaccttcttc ccgctcgaca tcctcccgaa gtgggcgctc gccgtcgcct ggtgcctgcc
     2041 gctcacccac atcacgatcc tcgtgcgctc ggccgtgctg gggatgggcc atccgatgct
     2101 tccggtgagc gtggcgtacg tgctgatcgt ggggctgggg ctgggcgcgt ggtcgttggt
     2161 gcggatgaag aagcggctga ttcagtagag cttcagcgag cggtgcacgc cgcgagctgc
     2221 tcctcgtcgt gcggttatat taatatataa cttaattttc acacgtccca ggacgtgttc
     2281 tttgtgcctg cctggcatgg tctcgtgtca gagaccgtgt caacacgaca caaagtacat
     2341 cttctggaag atgtaatgcc tgcggccctc gatcagggcc cgcgatcata gggatccgat
     2401 caggggcccg cgatcagggg tccgcgatca ggtgcccaga agctggccgg tcatggctgc
     2461 gctccgcggg cccgcgtcat ctcctgctcg aattccgcga gtctccggcg cgccgtcgcg
     2521 gcgagcgtgg cgtcgcctcc cgcgagcgag atggcgcggc gatagtggtg cgagatgctc
     2581 tccaccgcgg cggtgttgga aggatccgtg agacgcgccc gttcggcttc cgccaggtac
     2641 agccggattc gcgcggaatc ggccccggcc cggccggtct gataaaggta gcgcaggtcc
     2701 gtctccgccc gcgccaccgc gtttccgggg acgccggagt tccacacggg cggtagcctg
     2761 taggcgcgca gcacgttcgt ataggcgtca cccggcaccg ccgcgccgcg cggcgcggct
     2821 tcgggagccg cgttctggga cgccatgagc ttccgatcgc cctcggtccc aggaacccgg
     2881 agcgcttggg gcgccacctg ggtccggatc tcgggggcct ggtccttgag cgagacatcc
     2941 ccggcgccgc tcaccgctcc cgccgagggc ggcagtgcct tggcttcgga cttcgcgagg
     3001 agccggtccc ggtcgctctt cttggccgcg gtcttctcgc gcgacggcgc tacctcccgg
     3061 gatcgggccc cagaaggggc gccggcgaca ccgttcgagg attcctggcg ctcagtctgc
     3121 aaggaatccg cgcgcggttc gatgcccggc tcgcccaaca ccgcggggcg cggcgcccgc
     3181 acccactcgc tctgccgttg gaacagcacg accgccacga ccaccaccgc ggccgccgcg
     3241 accacgccgc cgccgattcc gatccacggc aggtgaaggc ggcggcgggg ctccagttcc
     3301 tcgctcgacg aatcgggccg acgccgctcc actcgcggaa cgtcgacgta gtccccgcgc
     3361 cgtgcgtggg tgcgcggctc gggcgccccg ggcgccgacg ccgcggtctc gcgcgtcgtc
     3421 accccgatca cggcctcgcg ccccggcgat ccgatcttgg ccatcaccga ctccgtcatc
     3481 cgctcgaagt actcttcccc cgcaccgtcg ggacgaaggc gcagctcggc ctccgccgcg
     3541 gacatctcct cgagccgttc gctgcactgg gcgcaggcca ggacgtgcgc ctcgatcagc
     3601 gtgcggcggt cggccgtgag ctcgccgtcg cggtaggcga acagctcctc cgatgtgggg
     3661 tggtgcgggt cgggcaggct tggactcatc ggggctccgc ttccctcaat tcgtgcagcg
     3721 cttcgatcag ggactcccgc gcgcgcgcca ggcgcgacat gacggtgccg acgggcacgg
     3781 ccagcgcctc cgcgatcgcc gcgtaggaga ggtcggcatc cacgcgaagc gccaggatca
     3841 tgcggtggtc ctcggacagc ttcgcgtagg cccgttccac ctgcacgcgc cgctccagcg
     3901 ccgcggcgtc ctgcgccggg tccgccacgc ggtccgcgag cggcgccgat ccgtccacgc
     3961 cgggctcgtc cagcggctcc gccgcgcggc gcttggcatc ccggatccag gtaagcgcgg
     4021 tgcgggccac gatggtgagg aaccaggggc cgaaggggcg gtccgccgcg aagcggtcca
     4081 gggcacggaa ggctttgacg tagctctcct ggagcacgtc gtcggcgtct tcatggttgc
     4141 gggtaatccg tcgcgccaag cgatagccgc tccgccgcga gcggcggacc agctcggcgt
     4201 aggcatccgc ctcaccatcg cgaatccggc gaacgagctc gccgtcgctc agcgaggagc
     4261 ggtcgggtcg ggccggtgcc atcgggcgcc gaatgtacta cgcccgaata cggaaggata
     4321 ttcccgtttc gtgtaatggg gcttgaggct tgggcctacg cgcgcggcgt cgccaacggg
     4381 cagccgggcg ctacgcgttt ttggcgcgag gggaggcaac cctctgggaa gtggtcgcct
     4441 cgcggcggct tcgccttgct cggctctaca cttccctccg ggttcttccc ctcgcgtcca
     4501 ggaatgcgtt gagtccgccg gaggcattgg cgaagacgag catcctgacc acattctctt
     4561 tgagtgtgaa atacatcaac gccgaggtag gtagatcctt ctggcgctga ttccttcacg
     4621 aatcaatttg ggggtgaggt gttgcgatga ggctggcgga cctcaacgtg atttgggacg
     4681 cgaggggagg aacccggagg gaagtgtaga gccgagcaag gcgaagccgc cgcgaggcga
     4741 ccacttccca gagggttgcc tcccctcgcg ccaaaaacgc gtagcgcccg gctgcccttc
     4801 gcaccggacg ctcgcatgct tctcgagacg actaccgccc cgcccgctta ctccggctct
     4861 ggcgacgcgt gagccacagg agacccaacc ccaccaccgc ggcccagacg atgatcttga
     4921 ccagcacgag ctatcacctc ctcgcgtcga gactcactgc gtagcgtttt gagcggcgat
     4981 ggcctgcgcg agctggatcg cggcaggtcc cagaatgacg atcaggagcg ccgggaagat
     5041 caggaacacg agcgggaacg cgagcttgat gcccg
//
LOCUS       DASSLZ010000301         7793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_493529, whole genome shotgun sequence.
ACCESSION   DASSLZ010000301 DASSLZ010000000
VERSION     DASSLZ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493529"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1675)
                     /locus_tag="VFM00_11430"
     CDS             complement(131..1675)
                     /locus_tag="VFM00_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7498901.1"
                     /translation="MIKEHPQYGSRIPAEELEFMRQQAEERARERKNRPPGTRPMGPR
                     PAGTGRYVPRDTGPRGPRPQGTPRYPGTPPRTPYRTGGPPNAGRAPYGKERAGGTRGG
                     APYPPRDRTSYPAPRDRASYPAQRERAPYPSQRERTPYPERRERFQGPPSARPERRPG
                     KPYRTGVQSAGEGPPRGVPIPEEPPLSKVRNILMGYQQSAVLLAAHSLGIFPEIHKRP
                     QVAADLARRLDADPRGMETLLEALVALGLLHRHGATYVLPRELATYLVPSDGGATGMV
                     EAAAELYAAWGDLARAVREGAPRVRLNSDALVEGDPARVRRYIRAVHTVSREAAGRVT
                     ELAPLLPGSTLLDVAGGSGVFAAEYARRTPELRATLFDLPPTIDVAREILREEGYEDA
                     VTLYPGDYRSDPLPGPVDALLLSNVLQTESEENALDLLRKAWEAIRPGGTLLVHGMMP
                     SGPGMPTVPQALFSLRMYLVFDSGRSWSVEQVSEWLARERFAVRATRALGAPFESTLI
                     LASRLE"
     gene            complement(1931..2383)
                     /locus_tag="VFM00_11435"
     CDS             complement(1931..2383)
                     /locus_tag="VFM00_11435"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET7498902.1"
                     /translation="MLEERISDDMKTSLKGGAADRLQTLRMMRSAILLEKKKDVSIQI
                     LPDPQVLQVLTSYAKKLREAAAEFEKLGQKDAAQKIQTELAVVQEYLPAPLTPDETKT
                     LVDAVVAELGASSPKDLGRVMKEVTARAQGRADGKALSDLARRALGMA"
     gene            2528..4102
                     /locus_tag="VFM00_11440"
     CDS             2528..4102
                     /locus_tag="VFM00_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7498903.1"
                     /translation="MIPWVLRPPFVRAARWPLLLLLFLVPAATPRPAHAVEERAWVSL
                     ELSGDFYDPEQALQDHFGYGVRGAGFLNRWVGVEGMFHRSSPKLEAPATGSGTFTHYG
                     AGLILTPDRFRWTLPYVVAGVGSAKVSRDDVAASGSHSAFHFGGGIVVRLGEKLGFRL
                     DGRDVTYKQEGGPGRDTRVNAVIASAGVTAFFAGRARDTDEDGVPDKRDRCNETPHGA
                     VVDASGCPLDTDADKVFDGLDKCPGTPAGAVVDAGGCPVDTDKDGVADGIDACNDTPQ
                     GVLVDAKGCSLDTDQDGVADGPDKCPGTPAGATVDASGCPVDSDGDGIADGIDICPAT
                     PAGAAVNAGGCPLELTRYERQMLQDWMIRLTDLAFVPDSARILPAGMARLDSVAVALR
                     QWPMLKIEIGAHVDDSVAPGYRVPLSTQRAQAVARYLFQQAPNLDPKNFSLTGYGDTE
                     PIVSNRSASNRARNRRIEFKVLNPNVLSQERQRRASFGSATPAPGTAPGTAPETPPPP
                     PPGGEAPSTPPPPGGH"
     gene            4306..6321
                     /locus_tag="VFM00_11445"
     CDS             4306..6321
                     /locus_tag="VFM00_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7498904.1"
                     /translation="MAPGPVAREVTIPPDLSDRQRIETERQHALSLLEATLESTTDGV
                     LIVDRNGKIIRFNERFVHLWRIPADIIRSRDDDRALETVLSQLENPEAFLSRVRQLYA
                     NPGAESHDVLRFKDGRVFERLSRPQRLRGEVVGRVWTFRDVTDRERALAEQRLAAARE
                     ATIARNLDSALFTFTLGEDLHVEEYEYFSLGAERLFGVPLDDLLGDPGFWDARIHPED
                     KEGVVRPALQGLLRLEPAMIEFRYESSKGIFRWHRSRFVPRREADGRVHVDAIETDVT
                     ERVGLEEQLRHAQKLEAVGQLAGGIAHDFNNILTVVIGYADLLLTRMRHDDQNRRAVE
                     EIHKGGDRAASLTRQLLAFSRRSPAQPRLLDLGASIRDLMPMLQRLAGEDIGFDLDVT
                     PGLGPVRVDPSQFEQVIVNLVVNARDEMPDGGILRIAVDRTEVTTAQVLGEPDLVAGS
                     YVRLRVADTGHGIDPAALEHIFEPFFTTKGLGRGTGLGLATVYGIVRQHRGRVSASNR
                     DQGAEFEILLPEVRSEGATSLAPARLAGVPEGRESVLLVEDDAALLTLGREVLSELGY
                     RVQAATSGRDALRLIEEQHPEIDILVTDVVMPEMGGRELAERIAVERPDLPILFVSGY
                     TRDAVLREELSGAAGPGIAFLEKPYTALVLARRVREMLDARKGAPTA"
     gene            complement(6356..7381)
                     /locus_tag="VFM00_11450"
     CDS             complement(6356..7381)
                     /locus_tag="VFM00_11450"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7498905.1"
                     /translation="MRIPYLPERILLTGSTGTVGRLIAPELAGFRAPMRLLARRGALP
                     GEPPPPAGAELRVGDLERPASLRGIADGCDVVVHAAERTGFVRFDRERQRRINVGGTE
                     SLLREAQSAGVKVFVLMGYTGTVQERSVREGDVDEETPPEGAYESDYVRMKTEAEALV
                     LEANGAAGMRTLVVSPGVLVAPGVDTILGGLIALYVAGELPYRILDDVWLAISDGRDL
                     GRFVAAAIGRGHGGRRYFATSGCLRLGDLYGRLAELSGIAPPRRRLPDLLVEELGLLT
                     PVLPQHSFLRQLVLPRELVLHLRRLAPLRNSKTLGDLGVAPMPVETTLADAVRSREAV
                     SREGTAG"
BASE COUNT         1065 a         2836 c         2788 g         1104 t
ORIGIN      
        1 gcggtccgga gcagggccgg ctggcggacc gctatcgact cgatgcggcg accggcgact
       61 ccggccgcca ggttctcggc caggacttcg aggaacggca gctccggcac ggaggcgtgg
      121 cccggagcgc ctactcgagg cgcgaggcga ggatcagcgt gctctcgaag ggagccccga
      181 gcgcacgggt ggcgcgcacc gcgaaacgct cgcgcgcgag ccactccgag acctgctcca
      241 cgctccagct gcggccggaa tcgaatacca ggtacatccg gaggctgaag agcgcctgcg
      301 ggacggtcgg catccccgga ccgctcggca tcatgccgtg cacgagaagg gtgcctccgg
      361 gccggatcgc ctcccaggcc ttgcgcagca gatcgagcgc gttctcctcg ctctcggtct
      421 gcagcacgtt cgaaagaagc agcgcgtcca cggggccggg cagcggatcg ctccggtagt
      481 cgccgggata gagcgtcacc gcgtcctcgt atccctcctc gcggaggatc tcgcgcgcca
      541 cgtcgatcgt gggcggcagg tcgaacaggg tggcccggag ctcgggcgtg cggcgcgcgt
      601 actcggcggc gaagactccc gacccgcccg ccacgtcgag gagcgtcgaa ccgggaagga
      661 gcggcgccag ctcggtgacg cgtcccgccg cctcgcggct cacggtgtgc acggcgcgga
      721 tgtagcgccg cacgcgcgcg ggatcgccct ccaccagcgc gtcgctgttc agccggaccc
      781 ggggcgcgcc ttcgcgcacc gcgcgggcca gatcgcccca tgcggcgtag agctcggcgg
      841 cggcctcgac catccccgtc gcgcccccgt cggacggcac gagataggtc gcgagctcgc
      901 ggggaaggac gtaggtggcg ccgtgacggt ggagcagccc gagcgcgacc agcgcctcga
      961 gcagggtctc catcccgcgc gggtcggcgt ccaggcgccg cgccaggtcg gcggcgacct
     1021 gcggccgctt gtggatctcg gggaagatgc cgagcgaatg cgcggccagg agcaccgccg
     1081 actgctggta gcccatcagg atgttccgca ccttcgaaag cggcggctcc tcgggaatcg
     1141 ggacgccgcg gggcggcccc tcgccggcgc tctgcacgcc ggtgcggtag ggcttgcccg
     1201 gtcgccgctc cggacgcgcg gagggcggtc cctggaaacg ctcccgccgc tcgggatagg
     1261 gagtccgttc gcgctgcgag gggtaggggg cccgttcgcg ctgcgccgga taggacgccc
     1321 ggtcgcgcgg cgctggatag gacgtccggt cgcgcggcgg atagggcgcg cctccgcgcg
     1381 ttcccccggc gcgctccttc ccatagggcg cgcggccggc gttcggcggc ccgcccgtac
     1441 gatacggcgt gcgcggcggg gttcccgggt agcgcggcgt tccctgcggc ctcgggccgc
     1501 gcggtcccgt gtcccgcggg acgtaccgcc ccgttcccgc cggacgcggt cccatcggac
     1561 gcgttccggg cgggcggttc ttgcgctcgc gcgcccgctc ctcggcctgc tggcgcatga
     1621 actcgagctc ttcggcggga atccggcttc cgtactgggg gtgttccttg atcaccggaa
     1681 cgtcgtcgcg gcccggcggt cgcccgcgcg ggggctttcc cggccggctg cctcgcgggg
     1741 cgggcggacg cggagagccg ggcgtggcgg acgagtgcgg gcgttcgcgc ttcttgcgat
     1801 tgggcttcat catattgtgg ggatgttcct catcgcggga ggccgcaatc gagcttgcgt
     1861 ctcgcgcgtg gtcgagtgga cggctcaggg cgtggcgccc gacacggcgc tcacgagccg
     1921 tgcgcgcggg tcacgccatg ccgagggcgc ggcgcgccag atcgctcagc gccttgccgt
     1981 cggcgcggcc ctgggcgcgc gcggtgactt ccttcatgac gcggccgaga tccttgggcg
     2041 aggaggcgcc cagctcggcg acgactgcgt cgaccaacgt cttcgtttcg tccggggtga
     2101 gcggcgcggg caggtattcc tgcaccaccg ccagctcggt ctggatcttc tgcgcggcgt
     2161 ccttctggcc gagcttctcg aactcggcgg ccgcttcgcg cagcttcttc gcgtaggagg
     2221 ttaacacctg caggacctgg gggtcgggaa ggatctggat cgagacgtct ttcttttttt
     2281 cgagaaggat cgcgctgcgc atcatccgca gcgtctggag ccggtccgcc gccccacctt
     2341 tgagtgaggt cttcatgtcg tcgctgatcc gttcttcgag cattcgcgca cccctcgtcc
     2401 accgcgcgcg gcactccgcg ccgctcggcg gcaaagtata acagaatctt gacataggcg
     2461 aggaaaacgg ccacactgcc gcgcgattcc ttccccctat atcgctcggt gattccggag
     2521 gcaagccgtg atcccttggg tgctccgtcc gcccttcgtc cgtgctgcgc gctggccgct
     2581 cctgctcctc ctgtttctcg tcccggccgc gacaccacgc ccggcgcacg cggtcgaaga
     2641 acgcgcgtgg gtctcgctgg agctctccgg agacttctac gaccccgagc aggcgcttca
     2701 agaccacttc ggctacggcg tgcggggcgc gggtttcctg aaccgctggg tcggcgtgga
     2761 ggggatgttc catcggtcct cgcccaagct ggaagcgccg gcgaccggga gcggcacctt
     2821 cacgcattac ggcgcggggc tgatcctcac gcccgatcgc tttcgctgga ccctccccta
     2881 cgtggtcgcc ggcgtcggat ccgccaaggt ctcgcgcgac gacgtcgccg cgagcgggtc
     2941 ccactccgcg ttccacttcg gcggcggcat cgtcgtgcgc ctgggcgaga agctcggttt
     3001 ccggctggat ggccgggacg tgacctacaa gcaggagggc ggcccggggc gtgacacgcg
     3061 cgtgaacgcc gtcatcgcca gcgccggcgt caccgccttc ttcgccggcc gcgcgcggga
     3121 caccgacgaa gacggcgtcc ccgacaagcg cgaccgctgc aacgagacgc cgcacggcgc
     3181 cgtggtggac gcgagcggat gcccgctcga caccgacgcg gacaaggtct tcgacggtct
     3241 cgacaagtgc cccggcactc cggcgggcgc ggtggtggac gcgggcggct gccccgtgga
     3301 cacggacaag gacggcgtgg cggacggcat cgacgcctgc aacgacacgc cgcagggagt
     3361 cctggtcgac gcgaaggggt gcagcctgga caccgaccag gacggcgtgg ccgacggtcc
     3421 ggacaagtgc cccggcacgc ccgcgggcgc gacggtggac gcgtcgggct gcccggtcga
     3481 cagcgacggc gacggcatcg ccgacggcat cgacatctgc ccggcgacgc ccgcgggcgc
     3541 ggcggtgaac gccggcggct gcccgctcga gctcacgcgc tacgagcggc agatgctcca
     3601 agactggatg atccgtctca ccgatctcgc gttcgtgccc gactcggcgc gcatcctccc
     3661 cgcggggatg gcccgtctcg attcggtcgc ggtcgcgctc cgccagtggc cgatgctcaa
     3721 gatcgagatc ggcgcgcacg tggacgattc ggtcgcgccg ggctatcgcg ttccgctctc
     3781 gacgcagcgc gcgcaggcgg tcgcccgcta tctcttccag caggctccga acctcgaccc
     3841 gaagaacttc tccctcacgg gctacggcga caccgagccg atcgtctcga accgctcggc
     3901 ctcaaaccgc gcccggaacc gccggatcga gttcaaggtc ctgaatccga acgtgctatc
     3961 gcaggagcgg cagcgccgcg cctccttcgg gtcggcgacg cccgcgcccg gcacggcgcc
     4021 gggcaccgct cccgagacgc ctccacctcc gcccccgggc ggcgaagcac ccagtacgcc
     4081 gccgcctccg ggcggccact agatcgaccc gcgggccgcg cgcacggagc gcggggcccg
     4141 cccgtgcggg acacgaggga ccgcgccatg ctcttttcct ccgcatggat cccggctgcc
     4201 gccctggccg ctggcgccgg actgcttgcg agcacgctcc tcgcgcgccg tcgtggcacc
     4261 cgacgtcccg tggtccctgc gccggagacg acgccaccgg cccccgtggc cccgggacct
     4321 gtcgcccgcg aggtcacgat cccgcccgac ctgtcggacc gccagcggat cgagaccgag
     4381 cgccagcacg cgctctcgct cctcgaggcg acgctcgagt ccacgaccga cggcgtcctg
     4441 atcgtggacc ggaacgggaa gatcatccgc ttcaacgagc gcttcgtgca tctctggcgc
     4501 atccccgcag acatcatccg aagccgggac gacgaccgcg cgctggagac ggtgctctcc
     4561 cagctggaga acccggaagc gttcctctcg cgggtccgcc agctctacgc caaccccggc
     4621 gccgaaagcc acgacgtgct tcgcttcaag gacggacggg tcttcgagcg cctctcccgg
     4681 ccgcagcggc tgcgcggcga ggtcgtggga cgggtctgga ccttccgcga cgtgaccgac
     4741 cgcgagcggg cgctggccga gcagcggctc gcggccgcgc gcgaggcgac gatcgcgcgc
     4801 aacctggact cggcgctctt caccttcacg ctgggcgaag acctgcacgt ggaggaatac
     4861 gagtacttct cgctgggcgc cgagcggctg ttcggcgtgc cgctggacga cctcctcggc
     4921 gatcccggct tctgggacgc gcgcatccac cccgaggaca aggagggcgt cgtccgtccc
     4981 gcgctccagg gacttctgcg cttggagccg gcgatgatcg agttccgcta cgaatcctcg
     5041 aaggggatct tccgctggca ccggagccgc ttcgtgccgc gccgcgaagc cgacgggcgc
     5101 gtgcacgtgg acgcgatcga gaccgacgtc acggagcgcg tgggcctgga ggagcagctc
     5161 cggcacgccc agaagctgga ggccgtgggt cagctggccg gagggatcgc ccacgacttc
     5221 aacaacatcc tgaccgtggt gatcggatac gccgacctgc tcctcacgcg catgcggcac
     5281 gacgatcaga accgccgcgc ggtggaggag atccacaagg ggggcgaccg cgccgcgtcg
     5341 ctcacgcgtc agctcctcgc cttcagccgg aggagcccgg cccagccgcg gctgctggac
     5401 ctgggcgcct cgatccggga cctcatgccg atgctgcagc ggctcgcggg cgaggacatc
     5461 ggcttcgacc tggacgtgac gccgggactg ggcccggtgc gtgtcgatcc gtcgcagttc
     5521 gagcaggtga tcgtgaacct ggtggtgaac gcgcgcgacg agatgcccga cggcgggatc
     5581 cttcggatcg cggtggaccg gaccgaggtg acgacggcgc aggtgctggg cgagcccgac
     5641 ctggtcgccg gctcctacgt ccgcctgcgg gtcgccgata ccggccacgg aatcgacccc
     5701 gcggcgctcg agcacatctt cgagccgttc ttcacgacga agggactggg ccgcggcacg
     5761 ggcctcgggc tcgccaccgt ctacggcatc gtgcgtcagc atcgaggccg cgtgagcgcc
     5821 tcgaaccggg accagggggc cgagttcgag atcctgctcc ccgaggtccg gtcggagggc
     5881 gcgacctccc tggccccggc gcggctcgcc ggcgttcccg aaggccgtga gtcggtgctc
     5941 ctggtcgagg acgacgcggc gctcctcacg ctcggacgcg aggtgctttc ggagctgggc
     6001 tatcgggtcc aggcggcgac cagcgggcgc gacgcgctcc gcctgatcga agagcagcac
     6061 cccgagatcg acatcctggt caccgatgtc gtgatgccgg aaatgggggg ccgcgagctg
     6121 gcggagcgga tcgcggtcga gcgcccggac cttccgattc tcttcgtttc ggggtatacg
     6181 cgcgacgcgg tgcttcggga ggagctctcc ggggcggcgg gaccggggat cgctttcctc
     6241 gagaaaccct acaccgccct cgtcctggcg cggcgcgtcc gcgagatgct ggacgcgcgc
     6301 aagggcgccc cgacggcgta gcgttcgcaa gggcgccccg acggcgtagc gggcgctacc
     6361 cggccgttcc ctcccggctg accgcttccc tcgaccgcac cgcgtcggcc agggtcgtct
     6421 ccaccggcat cggcgccacg ccgagatcgc cgagcgtctt cgaattccga agcggcgcga
     6481 gccggcgcag gtgcagcacc agctcgcgag gaagcaccag ctggcgcagg aaggagtgct
     6541 gcggcagcac gggggtgagc agcccgagct cctccaccag caggtcggga aggcgccgcc
     6601 gtggcggggc gatgccgctc agctcggcga gccgaccgta gaggtctccg agccgaaggc
     6661 agcccgacgt ggcgaagtag cggcggcctc cgtgcccgcg tccgatcgcc gcggcgacga
     6721 agcgcccgag atcgcgccca tcgctgatcg cgagccagac gtcgtcgagg atccgatacg
     6781 gcagctcccc cgccacgtag agcgcgatca gcccgcccag gatggtgtcc accccgggcg
     6841 cgacgaggac gccgggactc accacgaggg tgcgcatgcc cgcggcaccg ttcgcctcca
     6901 gcaccagcgc ctcggcctcg gtcttcatgc gcacgtaatc cgactcgtag gccccctcgg
     6961 gcggcgtctc ctcgtccacg tccccttccc gaaccgagcg ctcctgcacc gtgccggtgt
     7021 agcccatcaa cacgaacacc ttcacgcccg cgctctgcgc ctcgcgcagg agcgactcgg
     7081 tgccgcccac gttgatgcgg cgctgccgct cgcggtcgaa ccgcacgaaa ccggtgcgct
     7141 cggccgcgtg gacgacgacg tcgcagccgt ccgcgatgcc gcgcagcgac gcggggcgct
     7201 cgagatcgcc gacgcgaagc tcggcgccgg ccggaggcgg cggttcgccc ggaagggccc
     7261 cgcggcgcgc gaggagacgc atgggcgcgc ggaatccggc cagctcgggg gcgatcaggc
     7321 gcccgacggt gccggtcgaa ccggtgagga ggatgcgctc ggggagatag ggaattcgca
     7381 tggcgtcgcc gcgccgcggc gcggatgcat aactctaccg aatccggggc ggcggcggca
     7441 cttccgaatc gtcgccgcgg gctctcgcgc tcccccgcca ctccacttca cgcggcaggc
     7501 gaaacgcaat ttcgcgccct tgcgccccgc gcaggttgcg cggcgcgcgc tggctgaaac
     7561 cagccgctct caggccgagc ggcttggtct tggccggcgg acgcgggcca cgcgcgcgcg
     7621 cggcgctccc gtaagtcgag gccgtgtcgc atcctcgacg aagcgcgccg cgtcctgccc
     7681 ggcgacgcca cgcacccggc acgatggctg cacccgaccc tggcgtcggg taggcgtccg
     7741 ccctggacgc cggcggcccg agtgcacctg tttcttggtg ttctctgcgg cct
//
LOCUS       DASSLZ010000302        10608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1634095, whole genome shotgun sequence.
ACCESSION   DASSLZ010000302 DASSLZ010000000
VERSION     DASSLZ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10608
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1634095"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2425)
                     /locus_tag="VFM00_11455"
     CDS             complement(<1..2425)
                     /locus_tag="VFM00_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7498906.1"
                     /translation="MGEVYRATDLKLGRTVALKILPADPTREPEARQRFLQEARFASV
                     LNHPNIVTIYSVDEADGLDFIAMEYVEGETVRKRARREPFSPEQLIDTGIQCAEALGA
                     AHALGMLHRDIKSANIILTPRNQAKILDFGLAKRMSEAAAAQTDVTAEVDLTTAGIVM
                     GSPGYMSPEQARGETLDARTDIFSLGCTLYEAATGKVPFEGPSTLAVMHATWTQEPTP
                     PSKLRPELTPDIDAILARCMAKDRDDRYASARELADALAALRETDRTGTVALPRAAAA
                     RGPNNLPAALTAFIGRKRETAELKRLLAQTRLITLSGPGGCGKTRLAVQVATDLLTDL
                     PDGAWIVELAPLADPALVPHHVAAALGVREESGRPVAETLAEAVAAKRMLLLLDNCEH
                     LVGACAALASALLQAAPDLRVLATSQEPLGVPGEVTWRIPTLGVPDLAVGARSREAIG
                     RYEAVRLFLDRAAGVQPTFALTDQNAVAVANICRRVDGIPLAIELAAARVKVLAPEQI
                     LARLEDRFKLLTGGSRTALPKQQTLRAAVDWSYDLLTPAEKTLLNRLSVFAGGFTLEA
                     AEAVCADDALDTLDLLDTLTHLVDRSLVVPEEAGEGEVRYRLLETIREYGREKLEAAG
                     ERAAFRVRHAEHFLALAEQAEPELTGPGQATWLRRLSREHDNLRTALVTWLEVEGPPV
                     RGLRLAGALWRFWWMRGNWAEGRSRLAALLLATGDAAPAERTKALHGAAVLARTQGEY
                     RAAQRMLEEALALARARGDRALTGSLVFELGNVANEEADYALARSLYEEALAIRRELG
                     DRRGLSAALH"
     gene            complement(2670..3005)
                     /locus_tag="VFM00_11460"
     CDS             complement(2670..3005)
                     /locus_tag="VFM00_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HET7498907.1"
                     /translation="MGRRSRKERCMPAKKRAAAKPKKKTASKRKPSAAFMKPMQPSAT
                     LSEVVGAKAMPRTEVTKKLWAYIKKNKLQDAKKRTMINADDKLKAIFGGKKQVSMFEM
                     TKLVSKHLK"
     gene            complement(3141..3217)
                     /locus_tag="VFM00_11465"
     tRNA            complement(3141..3217)
                     /locus_tag="VFM00_11465"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3181..3183),aa:Gly,seq:gcc)
     gene            complement(3252..4841)
                     /locus_tag="VFM00_11470"
     CDS             complement(3252..4841)
                     /locus_tag="VFM00_11470"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HET7498908.1"
                     /translation="MAQRFRVAMLSAECVPFAKVGGLGDVTGALSRAVADLGCSVAVV
                     LPRYGDLALPEGATLELVGQVEVPVAGNDETALLYRLHQAGLPAHLHHFFLANEAWFG
                     RDGIYADPKTGTPFADNAERWVFFARAALEALRAAAYAPEVIHANDHQTSLAPAYLKT
                     LLADDPFFQMTATILAIHNLGYQGVYPPETMGAAGFPDDLFYPLSPFEFWGKMNFLKA
                     GIHFADVLVTVSERYAEEIQSGEEFGFGLQGVLNARRTDLVGILNGIDVKTWNPRTDR
                     HLAARYDAESLDAKERCTEVLLEEMGLSRAPRRPVFGMISRLVEQKGLDLIRAAADRL
                     FALPARWVFLGNGEREYEEFLETAARRHPDRVAFRRGFDDPLAHRIEAGADFFLMPSR
                     YEPCGLNQMMSMRYGTVPVVRATGGLADTVEPVARRGEEGTGILFEPYTAEGLIAAAE
                     EAVALFAEPAALARVRRNGMTRDFSWESSARKYIHLYRQANAARRMGSGFNRWLEAME
                     GEGRRAGPPSPPPLTRGKGGV"
     gene            complement(4844..5884)
                     /locus_tag="VFM00_11475"
     CDS             complement(4844..5884)
                     /locus_tag="VFM00_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4921 family protein"
                     /protein_id="HET7498909.1"
                     /translation="METPPDAPELRKDPIAGRWVMIAPDRGRRPAPAPPPSTPAAAGG
                     PCPFCPGNERHTPPEVDARRDPGSRPDAPGWRTRVVPNKFAALSAAGAPERAREGIYE
                     RMDGVGAHEVIIESPEHDAELSELPAERVAEVLEVCRDRARAHASDPRVRYVLPFKNY
                     GAWAGASQEHSHTQLLATPVIPNVAREELAECERRWHETGRCSFCAMIAHELESQARL
                     IARDDRFVALAPYASRFAYETWILPLRHESSFTSAERAELDALAAILSATLRRMRAAL
                     DRPCYNFLIHSAPARETALPWYHWHVEIVPKLSLASGFEWGTGAYINAVPPEEAAERL
                     RAPERAAVPPAS"
     gene            complement(5898..6542)
                     /locus_tag="VFM00_11480"
     CDS             complement(5898..6542)
                     /locus_tag="VFM00_11480"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7498910.1"
                     /translation="MRSSRGPGRARRPIAPAALLLALTAGLALGGCAHAPRHAGGAEP
                     VERDHEAPAPPTRRAELPPEPAPEERPPSGAPARLDPSEQITPEELATLPDPVPRGGA
                     AAPQPAPNAPEPSSGTLPPGSGLGPSGGETTAPAPSEGLWSVQIHASESRTEAERLGR
                     AAARRLGAELFIQREGSLYKVRLGAFATEADAQALRDRAIGAGYPGAFRTRFRP"
     gene            complement(6529..7593)
                     /locus_tag="VFM00_11485"
     CDS             complement(6529..7593)
                     /locus_tag="VFM00_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET7498911.1"
                     /translation="MRLHPVILAGGRGERFWPLSRRARPKQLLPLLSDRPMLADTLAR
                     LQGAASPRDTFVITARDLAGAVRDAVPEIPHHHIVGEPVGKNTAPAVALAAWWLRDAG
                     GDAVVAVLPSDHRVEPPERFRDELIRAGETALERGAIVTFGIPPTRPETGYGYIEVGA
                     PTAPGSPFSTVAAFREKPDLATASRYASDGRHLWNAGMFLFPPRVMLEEIGAHAPEIA
                     ALLPALPARLAEDEAALARYYQSAPSVSIDVAVMERSARALVARAGFAWDDLGSWVAL
                     DGAEATGRANVTRGRTILLDSEGVVAFADGGLVAAVGARDLVIVHTPDATLVCAKDRV
                     QEVRALVARLKAEPGGDAFL"
     gene            complement(7678..8286)
                     /locus_tag="VFM00_11490"
     CDS             complement(7678..8286)
                     /locus_tag="VFM00_11490"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET7498912.1"
                     /translation="MGAYRRFGKRALDLVLGSVLTLVALPVIAVAAVLVKLDSPGPVF
                     HRAVRVGRGGRKFTFLKLRSMQRNAEELRALLLHRNESQWPAFKLTNDPRVTRVGRLL
                     RKTSIDELPQLWHVVQGHMSLVGPRPPFPEEVERYEPWMLRRLAVRPGLTCLWQISGR
                     SDLSFEEWMRLDLEYVDRYSLGLDARILLLTVPAVISGRGAY"
     gene            complement(8399..>10608)
                     /locus_tag="VFM00_11495"
     CDS             complement(8399..>10608)
                     /locus_tag="VFM00_11495"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HET7498913.1"
                     /translation="LPAGATFTANATNTSGTFSWTPTNAQTGTTNVTFTASNALTGSA
                     TTVITVTNGDRAPVVTAPATASGAENTLITFTVSAVDPDGDAITTLSAAPLPAGASFT
                     SNAAHTSGTFSWTPSFAQSGSYNVTFTATNALSGSATTAITVTNVDRAPVVTAPATAS
                     GNEGTLISFTVTASDPDGDAISSLTGAPLPSGATFTANATNTSGTFSWTPSFTQSGSY
                     NVTFTATNALTGSASTSITVNNVDRAPVVTAPPTASGAEGTTITFTVTASDPDGDAIA
                     SLTAAPLPGTATFTANATNTSGTFSWATTLADAGTYNVTFTASNALSGSATTAITVSN
                     QNQAPTVTAPASASGNEGTLITFGVSATDPDGDHVTLTALGLPSGSTFTDNGDNTGTF
                     SWTPSFTQAGSYSVTFHGADGNGGTANATTSITVNNVDRAPIADAGGPYTGITGIAVS
                     FNGSGSSDPDGDALTYAWDFGDGGTSTAQSPTHAYAAAGVYNVTLTVTANGLSDTKAT
                     TATISDTFQAFAFTTGGNKTTSLGAGKPYTCIQIQPVGGSYSNTDVDLSSIRMFYPST
                     STTNSILADPTKTSVDGDKNSDGITEIAACFKKSDMRVLFTGLPAGSTPVTVKLTGNL
                     VGGGRFETTLDMIVKSNGSFLAASISPNPLNPQAKLTFSTTKPGAVRVQMFDPQGRLV
                     KTITDETSVQAGYHDFTIDGRSSTGSKLASGVYFVKVWSQFDGSEVKSITILK"
BASE COUNT         1530 a         3687 c         3837 g         1554 t
ORIGIN      
        1 tgtgcagggc cgccgagagc ccccgccggt cgcccagctc gcggcggatc gcgagcgcct
       61 cttcgtacag ggagcgggcc agggcgtagt ccgcctcttc gttggcgacg ttccccagct
      121 cgaagacgag cgatccggtg agcgcgcggt cgccgcgcgc gcgggccagc gcgagcgcct
      181 cctccagcat gcgctgggcg gcgcggtatt ccccctgggt ccgcgccagg acggccgcgc
      241 cgtgcagcgc cttggtgcgc tcggcgggcg ccgcgtcgcc ggtcgcaagg agcagggcgg
      301 cgaggcggga gcgcccctcg gcccagttcc cgcgcatcca ccagaagcgc cagagcgcgc
      361 ccgccagccg gagcccgcgc accggcgggc cctccacttc cagccaggtc accagggcgg
      421 tgcgcaggtt gtcgtgttcg cgggagaggc ggcgcagcca ggtcgcctgc cccggtccgg
      481 tcagctcggg ctcggcctgc tcggcgaggg ccaggaagtg ctcggcatgg cggacgcgga
      541 acgccgcgcg ctcccccgcc gcctccagct tctccctccc gtattcgcga atggtctcga
      601 ggagccggta gcggacttcc ccctcgcccg cctcctcggg caccaccagc gagcggtcca
      661 ccaggtgcgt gagcgtgtcg aggaggtcca gggtgtcgag cgcgtcgtcc gcgcacacgg
      721 cttcggcggc ctcgagcgtg aagccgcccg cgaagacgct cagccggttc aggagcgtct
      781 tctcggcggg cgtgagcagg tcgtaggacc agtccaccgc cgcgcgcagg gtctgctgct
      841 tggggagcgc cgtgcggctg ccgccggtca gcagcttgaa gcgatcctcc agtcgggcca
      901 ggatctgctc cggcgcgagc accttcacgc gcgccgcggc cagctcgatc gcgagcggaa
      961 tgccgtcgac gcggcggcag atgttcgcca cggcgaccgc gttctgatcg gtgagcgcga
     1021 acgtgggctg gaccccggcc gcgcggtcca ggaagaggcg taccgcctcg taccggccga
     1081 tcgcctcgcg gctccgggcg cccaccgcca gatcgggaac gcccagcgtg ggaatccgcc
     1141 aggtcacctc gccgggcacg ccgagcggct cctggctggt cgccagcacg cgaagatcgg
     1201 gagcggcctg gagcagcgcg ctcgcgagcg cggcgcacgc gccgaccagg tgctcgcagt
     1261 tgtccagcag gagcagcatc cgcttcgcgg cgaccgcctc ggccagcgtc tcggcgaccg
     1321 ggcgcccgga ctcctcgcgc acgcccagcg ccgccgccac gtggtgcggc acgagcgccg
     1381 gatcggcgag cggggccagc tccacgatcc aggccccgtc cggcagatcc gtgaggagat
     1441 cggtcgcgac ctgcacggcg aggcgcgtct tgccgcaccc gccggggccc gaaagcgtga
     1501 tcaggcgggt ctgcgcgagc agccgcttca gctcggcggt ctcccgcttc cggccgatga
     1561 acgcggtgag cgcggccggg aggttgttcg ggccccgcgc cgccgccgcg cgcgggagcg
     1621 cgaccgtgcc cgtccggtcc gtctcgcgga gcgcggccag cgcgtcggcc agctcgcgcg
     1681 cggaggcgta gcggtcgtcg cgatccttgg ccatgcagcg cgccaggatg gcgtcgatgt
     1741 ccggcgtcag ctcgggccgc agcttgctgg gcggcgtggg ctcctgggtc caggtcgcgt
     1801 gcatcacggc gagcgtgctc ggcccctcga agggaacctt gccggtcgcc gcctcgtaca
     1861 gcgtgcaccc gagggagaag atgtccgtgc gcgcgtccag cgtctccccg cgcgcctgct
     1921 ccggcgacat gtatccgggc gatcccatca cgatccccgc cgtcgtgaga tccacctcgg
     1981 ccgtgacgtc ggtctgggcg gcggcggcct ccgacatccg cttggccagc ccgaaatcga
     2041 ggatcttcgc ctgattcctc ggcgtgagga tgatgttcgc cgacttgatg tcgcggtgga
     2101 gcatgcccag cgcgtgggcg gccccgagcg cctcggcgca ctggatgccg gtgtcgatca
     2161 gctgctccgg gctgaagggt tcgcgccgcg cacgcttgcg aacggtctcc ccctccacgt
     2221 actccatggc gatgaagtcg aggccgtccg cctcgtccac ggagtagatg gtgacgatgt
     2281 tgggatggtt cagcacggag gcgaagcgtg cctcctgcag gaatcgctgg cgcgcttcgg
     2341 gctcgcgcgt cgggtccgcg ggcaggatct tgagcgccac cgtccggccc agcttcaggt
     2401 cggtcgcgcg atagacctcg cccatcccgc cgtggcccag cgagccctcg atgcggaaat
     2461 ggcccaggat gcgattggcg tccagcgtgc tcaggcaaaa ccctcgcgaa ggggaagcgg
     2521 tgacgtccgg gaacgtctga gtgtagcgga gcggaaggga ccctgggaac gaggtcgctc
     2581 cgggggcgga cgggggccca atcgaacgcc cccgccgcgg tgcttcgcgg cgggggcgcg
     2641 atacggggcg ccgtaggatc gaaagacgct tacttgagat gcttcgagac gagcttggtc
     2701 atctcgaaca tgctcacttg cttcttgcca ccgaagatcg ctttgagctt gtcgtccgcg
     2761 ttgatcatgg tgcgcttctt cgcgtcctga agcttgttct tcttgatgta cgcccacagc
     2821 ttcttcgtca cctcggtgcg cggcatcgcc ttcgcgccca ccacttcgga gagggtcgcc
     2881 gacggctgca tcggcttcat gaacgcggca ctgggcttcc gcttggaggc cgttttcttc
     2941 ttgggctttg ctgcggcgcg cttcttagct ggcatgcacc tctcctttcg tgaacgtcgg
     3001 ccgatccgaa catgtcggcg ccgaagggaa gtactagcag attcactgcg ggcgtacagg
     3061 gaaaaatggt gattttccga gggaaaaccg atgtttttcc caggagaatc gccgggaggg
     3121 cgggagccgg gagatgaaaa tggagcggaa gacgggattt gaacccgcga caaccacctt
     3181 ggcaaggtgg ggctctaccg ggctgagcta cttccgctca caggcaaatt gtcggtacgg
     3241 cggaggggat tctaaacccc cccttttcct cgcgtcaagg gaggcgggga cggcgggccg
     3301 gcgcgtctcc cctcgccctc catcgcctcc agccagcggt tgaatcccga tcccatcctg
     3361 cgggccgcgt tcgcctggcg gtagagatgg atgtacttcc gcgccgagga ctcccaggag
     3421 aaatcgcgcg tcatgccgtt tcgccggacc cgcgcgagcg cggccggctc ggcgaagagg
     3481 gccacggcct cctcggcggc ggcgatcaac ccctcggccg tatagggctc gaacaggatg
     3541 cccgtcccct cctcgccccg gcgcgccacc ggctcgaccg tgtcggccag cccgcccgtg
     3601 gcgcggacca cggggacggt gccgtagcgc atgctcatca tctggttcag gccgcacggc
     3661 tcgtagcgcg acggcatgag gaagaagtcg gcccccgcct cgatccggtg cgcgagcgga
     3721 tcgtcgaagc cgcgccggaa cgcgacccgg tcggggtggc gccgtgccgc ggtctcgaga
     3781 aactcctcgt actcccgctc cccgttcccg aggaagaccc agcgcgcggg gagcgcgaag
     3841 aggcggtcgg cggcggcgcg gatcaggtcc agccccttct gctccacgag ccgggagatc
     3901 atcccgaaca cggggcgacg cggcgcgcgc gagagcccca tctcctcgag gagcacctcc
     3961 gtgcagcgct ccttggcgtc caggctctcc gcgtcgtagc gcgcggccag gtggcggtcg
     4021 gtgcgcggat tccaggtctt cacgtcaatg ccgttcagga tgccgacgag gtcggtgcgc
     4081 cgcgcgttca gcaccccctg cagtccgaac ccgaactcct cccccgactg aatctcctcg
     4141 gcgtagcgct cgctcaccgt gacgagcacg tcagcgaagt ggatccccgc cttcaggaaa
     4201 ttcatcttcc cccagaactc gaacggcgag agcggataga agagatcgtc cgggaaaccg
     4261 gcggcgccca tcgtctcggg cgggtagacc ccctggtagc cgaggttgtg gatggccagg
     4321 atcgtcgcgg tcatctggaa gaagggatcg tcggcgagga gcgtcttgag ataagccgga
     4381 gcgagcgacg tctggtggtc gttcgcgtgg atcacctcgg gcgcgtaggc cgccgcgcgc
     4441 agcgcctcga gggcggcgcg cgcgaagaag acccagcgct cggcgttgtc ggcgaacggc
     4501 gttcccgtct tcggatcggc gtagatgccg tcccggccga accaggcctc gttcgccagg
     4561 aagaagtggt gcaggtgcgc cgggaggccc gcctggtgca gccgatagag gagcgccgtc
     4621 tcgtcgttcc ccgccacggg gacctcgacc tgtccgacca gctcgagcgt ggccccctcg
     4681 ggcagcgcga ggtcgccgta gcgcggaagg accacggcca ccgagcagcc gagatccgcc
     4741 acggcgcgcg agagcgcgcc ggtcacgtcg cccagcccgc ccaccttggc gaaggggacg
     4801 cactcggcgc tcagcatggc gacgcggaag cgctgggcca tcgctacgag gcgggcggta
     4861 ctgcggcccg ctcgggcgcg cgcagccgct cggccgcctc ttcgggcggc accgcgttga
     4921 tgtaggcgcc ggtcccccat tcgaagccgg aggccagcga gagcttggga acgatctcca
     4981 cgtgccagtg ataccacgga agggccgtct cgcgcgcggg cgccgagtgg atcaggaagt
     5041 tgtagcaggg gcggtcgagc gccgcccgca tgcgccggag cgtggcgctc aggatcgccg
     5101 cgagggcgtc cagctccgcg cgctccgccg aggtgaagga cgattcgtgc cggagcggaa
     5161 ggatccaggt ctcgtaggcg aaccgcgaag cgtagggagc gagcgcgacg aagcggtcgt
     5221 cgcgcgcgat gaggcgggcc tggctctcca gctcgtgggc gatcatggcg cagaaggagc
     5281 agcgccccgt ctcgtgccag cgccgctcgc attcggccag ctcctcccgc gccacgttcg
     5341 ggatcaccgg cgtggcgagg agctgcgtgt ggctgtgctc ctgcgacgct ccggcccagg
     5401 cgccgtagtt cttgaaggga aggacatagc gcacgcgcgg gtcggacgcg tgcgcgcggg
     5461 cgcggtcgcg gcagacctcc agcacctcgg ccacgcgctc cgcgggaagc tcggagagct
     5521 ccgcgtcgtg ctcgggggat tcgatgatca cctcgtgcgc ccccaccccg tccatccgct
     5581 cgtagatccc ctcccgggcg cgctccggcg cgcccgcggc gctcagggcg gcgaacttgt
     5641 tcggaacgac gcgcgtcctc catcccggcg cgtcgggacg cgatcccgga tcccgccgcg
     5701 cgtccacctc gggcggggtg tgccgctcgt tccccgggca gaaggggcaa ggaccgccgg
     5761 ccgcggcggg cgtggaagga ggcggcgcgg gggcgggacg ccggccgcgg tcgggcgcga
     5821 tcatgaccca gcgtcccgcg atgggatcct tgcgcagctc gggcgcgtcc ggcggcgtct
     5881 ccacgaggcg aagtttacta cgggcggaaa cgggtccgga acgcgccggg ataccccgcg
     5941 ccgatcgccc ggtcgcgaag cgcctgcgcg tccgcctcgg tcgcgaaggc gccgagccgg
     6001 accttgtaca gcgacccctc gcgctggatg aagagctccg ccccgaggcg ccgggccgcg
     6061 gcgcgcccca gccgctccgc ctcggtccgg ctctcggagg cgtggatctg cacgctccaa
     6121 agcccctccg agggcgctgg agcggttgtt tcgccgccgg aggggcctaa ccccgagccg
     6181 ggcgggagcg tgccggagga aggctccggc gcattcggag ccggctgggg tgcggccgcg
     6241 cctccccgcg gcaccggatc gggcagggtc gccagctcct cgggggtaat ctgctcggag
     6301 gggtcgaggc gcgcgggagc gccgctcggc gggcgctcct cgggggccgg ctccgggggg
     6361 agctcggcgc ggcgcgtggg aggcgcgggc gcttcgtggt cgcgctccac cggctcggcc
     6421 ccgccggcgt gccgcggggc gtgcgcgcac cccccgaggg cgagcccggc cgtcagcgcc
     6481 agaaggagcg ccgcgggcgc gatcggccgg cgggcgcgcc cggggcccct agaggaacgc
     6541 atccccgccg ggctcggcct tcaaccgggc caccagcgcg cgcacttcct gcacgcgatc
     6601 cttcgcgcag acgagggtgg cgtccggcgt gtgcacgatg acgaggtcgc gcgcgcccac
     6661 ggcggcgacc agcccgccgt cggcaaacgc gacgacgccc tcggagtcga gaaggatcgt
     6721 ccttccccgg gtcacgttcg ccctgcccgt cgcttcggct ccatccagcg cgacccagga
     6781 acccagatca tcccaggcga accccgcccg cgctacgagg gctcgcgcgc tccgctccat
     6841 caccgccacg tcgatcgaga ccgacggggc cgactggtag tagcgcgcga gggccgcctc
     6901 gtcctcagcg agccgcgcgg gaagcgcggg aaggagggcc gcgatctcgg gggcgtgcgc
     6961 gccgatctcc tccagcatga cgcgcggcgg aaagaggaac atccccgcgt tccagagatg
     7021 gcgcccgtcc gaggcgtagc gggacgcggt cgcgagatcg ggcttctcgc ggaacgcggc
     7081 gacggtcgaa aacggcgagc ccggtgccgt gggagcgccc acctcgatgt agccgtagcc
     7141 cgtctcgggt cgcgtgggcg ggatgccgaa ggtcacgatc gcgccgcgct cgagggcggt
     7201 ctcccccgcg cggatcagct cgtcccggaa gcgctccggc ggctccacgc ggtgatcgga
     7261 ggggagcacc gcgacgacgg cgtcgccgcc cgcgtcgcgc agccaccagg ccgcgagcgc
     7321 caccgcgggc gccgtgttct tccccaccgg ttccccgacg atgtggtgat gcgggatctc
     7381 cggcaccgcg tcccgcaccg cgcccgcgag gtcgcgcgcg gtgatgacga acgtgtcgcg
     7441 cggcgaggcg gctccctgaa gccgcgccag cgtgtcggcc agcatggggc gatcggagag
     7501 gagcggcagg agctgcttgg gccgggcgcg gcgcgagagc ggccagaagc gctcgccgcg
     7561 ccctcccgcc aggatcaccg ggtggaggcg catgggggga gccgtatcac accgggagga
     7621 taggggcaac cctagggcct cgggccgggc ccagccccgc cctcggcggg ccgccgctca
     7681 gtacgctccc cgccccgaga tcacggcggg cacggtgagc aggaggatcc gcgcgtccag
     7741 gcccagcgag tagcgatcca cgtactcgag atccaggcgc atccactcct cgaaggagag
     7801 gtcgctccgc ccggagatct gccagaggca ggtgaggccg gggcgcacgg ccaggcgccg
     7861 cagcatccac ggctcgtagc gctccacctc ctccggaaac ggaggacgcg ggcccaccag
     7921 gctcatatgc ccctggacca catgccagag ctgcggcagc tcgtcgatcg aggtcttccg
     7981 gagcaggcgc cccacccgcg tcacgcgcgg atcgttggtc agcttgaacg ccggccattg
     8041 cgattcgttc cggtgcagga gcagggcgcg cagctcctcg gcattccgct gcatggaccg
     8101 gagcttcagg aaggtgaact tgcgcccgcc gcgacccacg cgcacggcgc gatggaacac
     8161 cgggccgggc gagtcgagct tcacgaggac cgcggcgacg gcgatcaccg ggagcgccac
     8221 gagggtaaga acggacccga gaaccaggtc gagcgcccgc ttcccgaacc gacggtaggc
     8281 ccccatgccg cgcaatctac cacgcccgcc gcggcccgcg tccccgggga aaaaggaaca
     8341 gggccgcggc ttgcgccgcg gccctgagtt ccctacgacc gatcctcgaa cagcgggact
     8401 acttgaggat cgtgatgctc ttcacttccg atccgtcgaa ctgcgaccag accttcacga
     8461 agtagacgcc cgaggccagc ttggagccgg tcgaggaacg tccgtcgatc gtgaagtcgt
     8521 ggtacccggc ctggaccgaa gtctcgtccg tgatcgtctt caccagacgg ccctgcgggt
     8581 cgaacatctg gacccggacg gcgcccggct tggtcgtcga gaacgtgagc ttggcctgcg
     8641 ggttgagcgg gttcggcgaa atgctggccg ccaagaacga accgttggac ttcacaatca
     8701 tgtcgagcgt ggtctcgaag cgaccgccgc caacgagatt gccggtaagt ttgacggtga
     8761 cgggcgtcga accggccggg agacccgtga agagcacgcg catgtccgac ttcttgaagc
     8821 atgcggcgat ctccgtgatc ccatcggagt tcttgtcacc gtcgaccgac gtcttggtcg
     8881 ggtcggcgag gatcgagttc gtcgtcgagg tgctcggata gaacatccgg atgctcgaca
     8941 gatccacgtc cgtgttcgag tacgagccgc cgaccggctg gatctggatg cacgtgtacg
     9001 gcttgcccgc gcccagcgac gtggtcttgt tgccacccgt cgtgaatgcg aacgcctgga
     9061 acgtgtccga aatcgtcgcc gtcgtggcct tcgtgtcgga gaggccgttc gccgtcaccg
     9121 tcaaggtgac gttgtagacc ccggccgcgg cgtacgcgtg cgtcgggctc tgggcggtgc
     9181 tggtgccacc gtcaccgaag tcccacgcgt aggtcagcgc gtcgccgtcc ggatcggacg
     9241 aaccgcttcc gttgaagctc accgcgatgc ccgtgatgcc ggtgtacggg ccgcccgcgt
     9301 cggcgatcgg cgcacgatcc acgttgttca ccgtgatcga ggtcgtcgcg ttcgcggtgc
     9361 cgccgttacc gtcggcgcca tggaacgtca cgctgtagct gcccgcctgg gtgaagctcg
     9421 gcgtccagct gaaggtgccc gtgttgtcgc cgttgtccgt gaaggtcgaa ccggacggga
     9481 gaccgagtgc cgtgagggtc acgtggtcgc cgtcaggatc cgtcgccgac acgccaaagg
     9541 tgatcagggt gccttcgttg cccgaggccg aggccggagc cgtgaccgtc ggagcctggt
     9601 tctggttgct gaccgtgatc gccgtggtgg ccgaacccga gagggcgttc gacgccgtga
     9661 acgtcacgtt gtaggtgccc gcgtcggcca gcgtcgttgc ccagctgaac gtccccgagg
     9721 tattcgtggc gttcgccgtg aacgttgccg tgccgggaag cggagccgcg gtgagcgaag
     9781 cgatcgcgtc accgtccgga tcggacgccg tcaccgtgaa ggtgatcgtc gtgccttccg
     9841 cacccgaagc cgtcggcgga gccgtgacca ccggcgcccg gtcgacgttg ttcaccgtga
     9901 tgctcgtgga ggccgaaccc gtcagggcgt tcgtcgccgt gaacgtcacg ttgtaggagc
     9961 ccgactgcgt gaacgacggc gtccagctga acgtgcccga cgtattggtc gcgttcgccg
    10021 tgaacgtcgc gcccgaaggc agcggagcgc ccgtgaggct gctgatggcg tcaccgtccg
    10081 gatcggaggc cgtgaccgtg aagctgatca gcgtgccctc gttgccggag gccgtcgcgg
    10141 gagccgtaac caccggagcc cggtcgacgt tggtgaccgt gatcgccgtc gtcgccgaac
    10201 ccgagagggc gttcgtcgcc gtgaacgtca cgttgtagct gccggactgg gcgaagctgg
    10261 gcgtccagct gaacgtgccc gacgtatggg ccgcgttcga ggtgaacgac gcacccgcgg
    10321 gcagcggagc cgccgacagg gtcgtgatcg catcgccatc cggatccacc gccgacaccg
    10381 tgaaggtgat cagcgtgttc tcagcacccg aagccgtcgc gggagccgtg accaccggag
    10441 cccggtcgcc gttcgtgacc gtgatgaccg tcgtcgcgct gcccgtgagg gcgttcgacg
    10501 ccgtgaacgt cacgttggtc gtgccggtct gcgcgttggt cggggtccag ctgaacgtcc
    10561 ccgaggtgtt cgtcgcgttc gccgtgaacg tcgcgcccgc aggcagcg
//
LOCUS       DASSLZ010000303         4611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1112661, whole genome shotgun sequence.
ACCESSION   DASSLZ010000303 DASSLZ010000000
VERSION     DASSLZ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112661"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFM00_11500"
     CDS             <1..221
                     /locus_tag="VFM00_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET7498914.1"
                     /translation="TRGTVNEFFMGEKNPILITIPPGVWHGMKGIGTEPAMLVNTPTE
                     PYKYDQPDEYRADPHTNDIPYDWSRKDG"
     gene            248..1279
                     /gene="rfbB"
                     /locus_tag="VFM00_11505"
     CDS             248..1279
                     /gene="rfbB"
                     /locus_tag="VFM00_11505"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET7498915.1"
                     /translation="MAKAPDLSRAQFLVTGGAGFIGSNFVHHLRRRFPDARITVLDKL
                     TYAGNLANLGDVKDAPGFRFVKGDICDAATVAGAMEGADVVVNFAAETHVDRSIEDPS
                     HFVLTDTFGVYVLLEEARKRGVARFVQVSTDEVYGEILSGAADERAPLLARNPYAASK
                     IGGDRLAYAYSVTYGVPAVVTRCSNNYGPFQYPEKLIPLFVTNALEDKPLPVYGSGKN
                     TRDWIHVEDHCGALVAILEAADVEGETFNIAGGNERSVLEIAALLLERLKKPANLLQH
                     VTDRPAHDRRYAIDASHLEAKTGFRPQVDFQRGIEATIDWYVANRPWWEAIRSGAFQQ
                     YYERTYGSR"
     gene            1292..2800
                     /locus_tag="VFM00_11510"
     CDS             1292..2800
                     /locus_tag="VFM00_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET7498916.1"
                     /translation="MRRLVRSTAILGLGSVATVVAAIVRAKILAARLGPEGTGVLAQL
                     ASLTAVLVPLATLGIGNGLVAMIAEARAANDFARVHALTRLGSRLAWGVGGGLALLAA
                     LASPWLAAGIYRDSAFTWAILLGAASVPLSAVASVNISMLQGHQAVRSMAGLNVAIAV
                     AQIATIVPLAFLWGVPGAVAQLVVITAIWAWWSGRLLAPHTPRAPRAPSPPGPGAGTV
                     PAAAAAKPLLRPLLRYGISSLLVGLSSTLTLLVLRSMLVDKLGLPANGIYQVCVGVSG
                     LLMPTILNAITANVWPEIAALPYDADVPPVMRSGVRLAFLLTTGFCAAILVGAPIWVP
                     LFYSGRFLPALDLLPIQFLGDYFRAAAWMFGIWLVPRNRLRPWVLFDVVYGVTLLVAF
                     VLLVDRVGLKSVVIGYVLAHMTHAGLHYWLARRSTGFKLGPDNRRLLGASLALLLGLL
                     AWTPKNLVGVGLGGLALVVWAAIVVRRREWVALLEVGRRRLAISERERPSPE"
     gene            complement(2769..3542)
                     /locus_tag="VFM00_11515"
     CDS             complement(2769..3542)
                     /locus_tag="VFM00_11515"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7498917.1"
                     /translation="MKKKSEAGYNERLFSGDLRGQLHRSRFRWLRESARRVNAPCRDV
                     LELGCFDGRSIAHLPHRPSTYLGLDANWEGGVDLGRSLWRDDPGVRFEICSTPLELRA
                     FVAGRRFDTTLSLETLEHVAPDLLDSYVEEMAKVTTGWFIATVPNEKGPVFLAKYAMK
                     RLFGDYFHYRPSEVLAAFLGRMDRVRRDDHKGFDYDTVVRLIGEHFDLVEVSPYPFRR
                     LPKWTGFGIGIVAKARAVSPSAVGSGTAALTPETGAPAR"
     gene            3692..>4611
                     /locus_tag="VFM00_11520"
     CDS             3692..>4611
                     /locus_tag="VFM00_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HET7498918.1"
                     /translation="MAIGTGAFVPDAFGQSGSRSAPSDVPQALPTSDIMPPIAGRVDP
                     ATYRVGPGDEFALRYSDLLEPRIVRVGPAGDLVLPDVGSLQVAGLTLEELEARVRDRM
                     RPYVRGKGFSFSLYRPRRFRMYVTGEVERPGTVTLQAPVRASEAIEAAGGITAGGARR
                     GIQVRRGAETIPVDLVLASRAGALSADPLVFESDVLFVPPAGRHVDVWGSVAHPGRYD
                     FVPGDRLSAILALAGGPLPEAALADAVLDRFDAEGRSERTPAPLDQAIASPGGAPDLP
                     VQEGDRLYVPSRVRWMRGDAVFVLGEVARPG"
BASE COUNT          636 a         1640 c         1589 g          746 t
ORIGIN      
        1 ccacgcgcgg cacggtgaac gagttcttca tgggggagaa gaaccccatt ctgatcacga
       61 tcccgcccgg cgtgtggcac gggatgaagg gcatcggcac cgagcccgcg atgctcgtga
      121 acacgccgac cgagccctac aagtacgacc agcccgacga gtaccgcgcc gatcctcaca
      181 cgaacgacat cccctacgac tggtcgcgca aggatggctg agcgccgccg gacgcgcgga
      241 ggccgccgtg gctaaggctc ccgatctttc gcgcgcgcag ttcctcgtca cggggggcgc
      301 gggcttcatc ggctcgaact tcgtccacca cctgcgccgc cgctttcccg acgcgcggat
      361 caccgtgctg gacaagctga cctacgcggg gaatctcgcg aatctgggcg acgtcaagga
      421 cgcgcccgga ttccgcttcg tgaaagggga catctgcgac gccgcgaccg tggccggcgc
      481 gatggagggc gccgacgtcg tcgtgaactt cgcggccgag acgcacgtcg accgctcgat
      541 cgaggacccc tcgcacttcg tcctgaccga tacctttggc gtctacgtgc tgctggaaga
      601 ggcgcggaag cgtggtgtcg cgcgcttcgt ccaggtctcc accgacgagg tctacggcga
      661 gattctgagc ggcgcggccg acgagagagc accgctcctc gcgcggaatc cctacgcggc
      721 ctccaagatc ggcggcgacc ggctggccta cgcctattcc gtgacctacg gcgtcccggc
      781 cgtcgtgacg cgctgcagca acaactacgg gcccttccag tacccggaga agctgatccc
      841 gctcttcgtg accaacgccc tcgaggacaa gccgctcccg gtctacggca gcgggaagaa
      901 cacgcgcgac tggatccacg tcgaggatca ctgcggcgcg ctggtggcca ttctcgaggc
      961 cgccgacgtg gaaggggaga ccttcaacat cgcgggggga aacgagcgct ccgtgctcga
     1021 gatcgctgcc ctcctgctcg agcggctgaa gaagcccgcc aacctcctcc agcacgtgac
     1081 cgatcgcccc gcgcacgacc ggcggtacgc gatcgacgcg tcccatctcg aagcgaaaac
     1141 cggcttccgc ccccaggtcg atttccagcg cgggatcgag gcgacgatcg actggtacgt
     1201 ggcgaaccgc ccgtggtggg aagcgatccg ctcgggcgcg ttccagcagt actacgaacg
     1261 gacctacggg tcccggtagc cacccggggc cgtgcgccgg ctcgtccgct ccaccgcgat
     1321 cctcggtctc ggatccgtcg cgaccgtcgt ggccgccatc gtccgcgcga agatcctcgc
     1381 cgcgcgactc ggccccgagg ggaccggcgt cctcgcccag ctcgcctccc tgaccgcggt
     1441 cctggtcccg ctcgccacgc tcgggatcgg aaacgggctc gtcgccatga tcgccgaggc
     1501 gcgcgccgcg aacgacttcg cgcgcgtgca cgccctgacg cgcctgggat cccgactcgc
     1561 gtggggggtc ggagggggac tggccctcct cgccgcgctc gcttccccct ggctggccgc
     1621 ggggatctac cgcgattcgg cgttcacgtg ggcgatcctc ctgggcgcgg cgtcggtgcc
     1681 gctctcggcc gtggcctcgg tgaacatctc gatgctgcag gggcaccagg ccgtgcgctc
     1741 catggcggga ctcaacgtgg cgatcgccgt cgcgcagatc gccacgatcg tcccgctcgc
     1801 gttcctatgg ggcgtgccgg gcgcggtggc gcagctcgtc gtgatcacgg cgatctgggc
     1861 gtggtggagc gggcggctgc tcgcgcccca taccccgcgc gcgccgaggg cgccgagccc
     1921 gcccggtccg ggcgcgggaa ccgtgcccgc cgctgcggcg gcgaaaccgc tgctccgtcc
     1981 cctcctgcgc tacggcatct cctcgctgct ggtcggcctc tcctccacgc tgacccttct
     2041 cgtgctgcgc tccatgctgg tggacaagct ggggctgccc gcgaacggca tctaccaggt
     2101 ctgcgtcggc gtgtcggggc tcctcatgcc cacgatcctg aacgcgatca cggccaatgt
     2161 gtggccggag atcgcggcgc ttccctacga cgccgacgtc cccccggtga tgcggagcgg
     2221 cgtgcggctc gcgttcctcc tcacgaccgg cttctgcgcc gcgatcctcg tcggcgcgcc
     2281 gatatgggtc ccgctcttct acagcggccg cttcctcccc gcgctcgacc tcctgccgat
     2341 ccagttcctg ggcgattact tccgcgccgc ggcctggatg ttcggcatct ggctcgtgcc
     2401 gcgaaaccgg cttcgcccct gggtgctttt cgacgtggtc tacggtgtga cgctgctggt
     2461 cgcgttcgtg ctcctggtgg accgcgtggg gctgaagagc gtggtgatcg gctacgtact
     2521 ggctcatatg acgcacgccg ggctccacta ctggctggcg cggcgctcga cggggttcaa
     2581 gctggggcct gacaaccgcc ggctgctcgg cgcctcgctg gcgctcctct tggggctgct
     2641 cgcctggacg cccaagaacc tggtcggcgt cggactgggg ggactcgccc tcgtcgtatg
     2701 ggcggcgatc gtcgtgcgcc ggcgggagtg ggtggcgctg ttggaggtgg ggcgccggcg
     2761 gctcgcgatc agcgagcggg agcgcccgtc tccggagtga gcgccgcggt accggagccc
     2821 accgccgagg gcgagacggc gcgggccttc gcgacgatgc cgattccgaa gccggtccac
     2881 ttgggaaggc ggcggaaggg atacggagag acctcgacga gatcgaaatg ctccccgatg
     2941 agccggacga cggtatcgta atcgaacccc ttgtgatcgt cccggcgaac ccggtccatc
     3001 cgccccagga acgccgccag cacctccgag ggccggtaat ggaagtaatc gccgaacagc
     3061 cgcttcatgg cgtacttggc caggaagacc gggcccttct cattgggaac ggtcgcgatg
     3121 aaccagccgg tggtgacctt ggccatctcc tcgacgtacg aatccaggag atccggcgcg
     3181 acgtgctcca gggtttcgag ggagagggtc gtgtcgaagc gccgccccgc gacgaaggcg
     3241 cgcagctcga gcggggtgga gcagatctcg aagcgcaccc ccgggtcgtc ccgccagaga
     3301 gaccggccga ggtccacgcc cccttcccag ttcgcgtcca gccccaggta ggtcgagggg
     3361 cggtggggga gatgggcaat ggagcggccg tcgaagcatc cgagctccag gacgtcacgg
     3421 cagggggcgt tcaccctccg cgcgctctcg cgcagccatc gaaaccgcga tcgatggagc
     3481 tgtccacgga gatcgccgga gaagaggcgc tcgttgtagc cggcttcgct cttcttcttc
     3541 atcgggaatc tcggttcctg cggtccctgg cgacggaatg aggcccttgg acctgtccac
     3601 ggcccatggc atgattgtcg acttgatccc accctgcgta tcgaacgctc gttgcgggcg
     3661 ccggttcctc ccagcgctgg ccgtggtcct catggccatc ggcaccgggg ccttcgtgcc
     3721 cgacgcgttc gggcaatccg ggtcccggag cgcgccatcg gatgtcccgc aggcgctccc
     3781 cacgtccgac atcatgccac ccatcgcggg gcgcgtcgac ccggccacgt accgcgtcgg
     3841 cccgggcgac gagttcgcgc tccgctactc ggatctgctc gagccccgga tcgtccgcgt
     3901 tggtcccgcc ggcgacctcg tgctccccga cgtgggctcc ctgcaggtgg ccgggctcac
     3961 gctggaggag ttggaagcgc gggttcggga ccggatgcgc ccctacgtgc gcgggaaggg
     4021 gttcagcttt tccctgtacc gcccgagacg ttttcgcatg tacgtgaccg gggaagtcga
     4081 gcgtcccggc accgtcacgc tgcaggcgcc ggttcgcgct tcggaagcga tcgaggcggc
     4141 gggggggatc accgcgggcg gagctcgacg cgggattcag gtgcgccggg gcgccgagac
     4201 gatcccggtg gatctggtcc tcgcctcgcg ggccggcgcg ctttcggccg atccgctcgt
     4261 tttcgagagc gacgtcctct tcgtgccccc cgcgggacgc catgtggatg tgtggggatc
     4321 ggtggcgcac ccgggccgct acgatttcgt tcccggcgac cgcttgagcg ccatcctggc
     4381 cctcgcgggc ggcccgcttc ccgaagcggc gctcgccgac gccgtgctgg accgattcga
     4441 cgccgagggg cgctccgagc gcacgccggc gccgctcgat caggcgatcg cctcgcccgg
     4501 cggcgcgccg gatcttcccg tccaggaagg ggaccgcctc tacgttccct cccgcgtccg
     4561 ctggatgcga ggcgacgccg tgttcgtgct cggcgaagtg gcccggccgg g
//
LOCUS       DASSLZ010000304         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_495940, whole genome shotgun sequence.
ACCESSION   DASSLZ010000304 DASSLZ010000000
VERSION     DASSLZ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495940"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFM00_11525"
     CDS             <1..939
                     /locus_tag="VFM00_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET7498919.1"
                     /translation="DAHLRQIIDRIISRVGRRIDESSPLVDARLPDGSRVNAIIPPSA
                     LDGPILSIRRFRRRALSIENMLELGSMTERMAVMLQGVIRARINALITGGTGSGKTTL
                     LNILSRSIPDGERIVTIEDSAELQLQQPHVVRLETRPPNLEGKGTITQRDLVRNALRM
                     RPDRIVVGEVRGDEVLDMLQAMNTGHDGSLTTLHSNGPRDALHRMENLVLMAGHSLPD
                     KAIREQIASALDCVIHVSRLPDGSRKVLSVQEIVGMEGPVVTMQEIFRFVRTGTKSDG
                     SIEGRFEATGVVPHFAERVELAGTNLGGLFDRGVVK"
     gene            936..1904
                     /locus_tag="VFM00_11530"
     CDS             936..1904
                     /locus_tag="VFM00_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7498920.1"
                     /translation="MIYLFVAVLLFGATAAIVFALNLRKQEQGTRSLRDRIGVQAPSR
                     GRATGGAALAIERDTRMSSMPFLDRFLRKLTVARGLELLLYQAGSSMRVGMFLLIMAA
                     GAMIAYLVGYVVFARLLHALVFMVVGGVAPLLILHHKKSVRMRAFSEEFPDALDLLVS
                     ALRAGISFSSAMQIVADESPEPIRSEFAIVVEEQALGLEMREALTNMANRVDSLDLKF
                     FVTAVVLQRDTGGNLTEVLDNTSRLIRDRFRILGDIQTFTAQGRLTGAILTCLPLGLA
                     IFMVIVAPEYFHKMWDLPSGRAVLGFGALMQLLGSLAIRQIVNIKV"
     gene            1909..>2735
                     /locus_tag="VFM00_11535"
     CDS             1909..>2735
                     /locus_tag="VFM00_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7498921.1"
                     /translation="MFLISTILLAFALAIGTLAIYQVFVRDRDPVAVRLRDLRSRALA
                     ARPNLDPDTPSPIAGLLATLGGFLPTSDSNEALQSGLVRAGIRDPKASLVFLGAKVVG
                     AVVVGIGWIAVNFAMARPIGSIVFSGGIAAAVGFYLPTIWLYMKGSSRQGQIQTALPD
                     ALDLLVVCVEAGLGLNAAIERVGREITLASPALSDELLVVGQEIRTGLSRSDALRRLA
                     RRTGVEDLYALTAMLIQADKLGTSVAQSLRSHAESMRTKRRQRAEQAARKAGIKLAFP
                     "
BASE COUNT          404 a          960 c          920 g          451 t
ORIGIN      
        1 gacgcgcacc tccggcagat catcgaccgc atcatctcgc gggtgggacg ccgcatcgac
       61 gaatcgagcc cgctcgtgga cgcgcgcttg ccggacggct cgcgcgtgaa cgcgatcatc
      121 cccccctcgg cgctggacgg accgatcctg tcgatccgcc gcttccgccg ccgggcgctc
      181 tccatcgaga acatgctcga gctgggctcc atgaccgagc ggatggcggt catgctccag
      241 ggcgtcatcc gcgcccgcat caacgcgctg atcacgggtg gcaccggctc gggcaagacg
      301 acgctcctga acatcctctc gcggtcgatt cccgacggcg agcgcatcgt gacgatcgag
      361 gactcggccg agctccagct ccagcagccg cacgtggtgc gcctggagac ccgcccgccg
      421 aacctggaag ggaagggaac gatcacccag cgcgacctgg tgcggaacgc gctgcgcatg
      481 cggcccgacc ggatcgtggt cggcgaagtc cgcggcgacg aggtgctgga catgctccag
      541 gccatgaaca cgggccacga cgggtcgctg acgacgctcc actccaacgg gccgcgggac
      601 gcgctgcacc gcatggagaa cctggtgctg atggcggggc actcgctgcc cgacaaggcg
      661 atccgggaac agatcgcgtc cgcgctcgac tgcgtgatcc acgtgtcgcg cctcccggac
      721 gggtcgcgaa aggtcctcag cgtgcaggaa atcgtgggca tggagggtcc cgtggtgacg
      781 atgcaggaga tcttccggtt cgtccgcacc gggaccaagt cggacggaag cattgaaggg
      841 cgtttcgagg ccaccggcgt cgtgccgcac ttcgcggagc gcgtcgagct ggccggaacc
      901 aatctcggcg gcctcttcga ccggggggtg gtgaaatgat ctacctgttc gtagcggtac
      961 tcctcttcgg cgccaccgcg gcgatcgtct ttgccttgaa cctgcgcaag caggagcagg
     1021 gcacgcgcag cctgcgggac cgcatcggcg tccaggcccc ctcgcggggg cgggcgacgg
     1081 gcggagccgc gctcgcgatc gagcgcgaca cccggatgag ctcgatgccg ttcctggatc
     1141 gattcctccg gaagcttacc gtcgcccgag ggctcgagct cctgctgtac caggcgggct
     1201 cctcgatgcg ggtgggcatg ttcctgctga tcatggcggc cggcgccatg atcgcgtacc
     1261 tcgtcgggta cgtcgtgttc gcccggctcc tccacgcgct ggtgttcatg gtggtgggcg
     1321 gcgtcgctcc ccttctgatc ctgcatcaca agaagagcgt tcgcatgcgc gcgttctccg
     1381 aagagttccc ggacgcgctc gacctcctgg taagcgcgct gcgcgcgggc atctcgttct
     1441 cctcggccat gcagatcgtg gccgacgagt cgccggagcc gatccggtcc gagtttgcca
     1501 tcgtcgtgga ggagcaggca ttgggtctcg agatgcgcga ggccctgacg aacatggcga
     1561 accgggtcga ctccctcgac ctcaagttct tcgtgacggc ggtggtgctc cagcgggaca
     1621 cgggcggcaa cctgaccgag gtgctcgaca acaccagccg cctcatccgc gaccgcttcc
     1681 ggatcctcgg cgacatccag acgttcacgg cgcaggggcg gcttaccggc gccatcctca
     1741 cctgcctgcc gctcgggctc gcgatcttca tggtgatcgt ggcgcccgag tacttccaca
     1801 agatgtggga ccttccgagc ggtcgggcgg tgctcggatt cggcgcgctc atgcagctcc
     1861 ttggatcact cgcgatccgc caaatcgtca acatcaaggt gtgatgccat gttcctgatc
     1921 tcgacgatcc tgctcgcctt cgctctcgcc atcggcaccc tcgcgatcta tcaggtgttc
     1981 gtgcgggacc gcgacccggt cgccgtccgg ctccgcgacc tgcgctcgcg cgcgctcgcg
     2041 gcacgtccca atctggatcc cgacacgccg tcgccgatcg cggggctcct ggccacgctc
     2101 ggcggcttcc ttccgacctc ggacagcaac gaggcgcttc aatccggcct ggtgcgggcc
     2161 ggcattcggg accccaaggc gagcctggtg ttcctgggcg ccaaggtcgt gggcgccgtg
     2221 gtcgtgggaa tcggctggat cgccgtgaac ttcgccatgg cccggcccat cggctcgatc
     2281 gtgttctcgg gcggcatcgc agccgccgtg ggcttctacc tgccgaccat ctggctctac
     2341 atgaagggca gctcgcgcca gggccagatc cagaccgcgc tccccgacgc cctcgacctg
     2401 ctggtcgtct gcgtggaggc gggccttggc ctgaacgccg cgatcgagcg ggtgggccgc
     2461 gagatcacgc tggcgagccc ggccctgagc gacgagctgc tcgtggtcgg gcaggagatc
     2521 cgcaccggcc tctcgcgctc cgacgcgctc cggcgcctgg cgcgtcgcac cggcgtggag
     2581 gatctgtacg ccctcaccgc catgctcatc caggccgaca agctcggaac gagcgtcgcg
     2641 cagtcgctgc gctcgcacgc cgagtcgatg cgcaccaagc gccgccagcg cgcggagcag
     2701 gccgcgcgga aggcgggcat caagctcgcg ttccc
//
LOCUS       DASSLZ010000305         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_226216, whole genome shotgun sequence.
ACCESSION   DASSLZ010000305 DASSLZ010000000
VERSION     DASSLZ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226216"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFM00_11540"
     CDS             <1..183
                     /locus_tag="VFM00_11540"
                     /inference="COORDINATES: protein motif:HMM:NF036850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7498922.1"
                     /translation="GGVVLDLNYGSRARAAREEARAMGMRYEDGLALLVEQGALSYEF
                     WTGEPAPREAMREALG"
     gene            180..998
                     /locus_tag="VFM00_11545"
     CDS             180..998
                     /locus_tag="VFM00_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET7498923.1"
                     /translation="MISARLRSGSGAGPALSALAADALDFLVGRSCLGCGGALPRGAA
                     VCDACDARVPRTGTVFCLRCMQEGNAPSDATRPGGGCPRHGSERLLLAGPAFEPPLDR
                     VVRAFKYSGAREAHRWLTALLPEPPGRGTPAWREYLLVPAPLHPARRAWRGFDQASLL
                     AATAGAAWGIPVLPALERKRDTPAQARVDGALRRENVRQAFALAADAGRALRARPVLL
                     VDDVATTGSTLLEAASALDAAAPSWVLSLAFAHGGLPGAPEPAFSTRVAAAPPV"
     gene            1098..>2502
                     /locus_tag="VFM00_11550"
     CDS             1098..>2502
                     /locus_tag="VFM00_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HET7498924.1"
                     /translation="MEKLRLLDEAYLPTVTKPSRYTGSFLHALRKDPAKASVRVLLCF
                     PDLYEIGMSYLGLKILYHLINRREDGLAELCFTPWGDMEKLMRAEGIPLYSLESRTPA
                     RAFDLIGFSLQYELAYTNVLTMIDLAGMPLRTKDRREDDPIVIAGGLCTVNPEPIAPF
                     IDAFVVGDGEEVMMELCDIAAERRRTKMPRAEALRRMAALEGVYVPALFAEVKNPWGF
                     IAREPLPGTPVARPRFRFVPSLDPSYVPEKPLISPTEITHDRLGVEVMRGCTRGCRFC
                     LAGYMNRPLREKRADQVAKEVQCGIVHAGWDEVSLLSLSTTDYKQLPVVLHELDRFTN
                     ELGVSVSLPSTRVGTLAPSVADKIGQGRKGSITFAPEAGTQRMRDVINKGIDEAELEY
                     SVKLARDQGWGGIKFYFMIGLPTEMDEDVDGIAETLRKSQEWARGGNKRMHFNVGISP
                     HVPKPHTPFQWEIQDDME"
BASE COUNT          350 a          874 c          900 g          378 t
ORIGIN      
        1 ggcggcgtgg ttctcgacct gaactatggc tcgcgcgcgc gggccgcgcg cgaggaggcg
       61 cgggcgatgg ggatgcggta cgaagacggg ctcgcgctcc tcgtggagca gggggcgctt
      121 tcgtacgagt tctggacggg ggagccggcg ccgcgcgagg cgatgcgcga ggcgctcgga
      181 tgatttcggc gcgcctccgc tccggaagcg gagcgggtcc ggcgctctcg gccctcgcgg
      241 cggatgccct cgacttcctc gtgggccgct cctgcctggg atgcggcggg gcgctcccgc
      301 gcggcgccgc cgtgtgcgac gcctgcgacg cgcgggtgcc gcgcacgggg acggtgttct
      361 gccttcgctg catgcaggag gggaacgcac cgtccgacgc gacgcgaccc ggcggcggct
      421 gcccgcgcca tggatcggag cgcctcctgc tcgccggccc cgccttcgag ccgccgctcg
      481 atcgcgtcgt ccgggccttc aagtactcgg gcgcgcgcga ggcgcatcgc tggctaaccg
      541 cgctcctgcc ggagccgccc gggcgcggca cgcccgcgtg gcgggagtac ctgctcgtgc
      601 cggcgccgct ccatccggcg cggcgcgcgt ggcgcggctt cgaccaggcg tcgcttctcg
      661 cggcgaccgc cggcgcggcc tggggcatcc cggtgcttcc ggccctggaa cggaaacggg
      721 ataccccggc tcaggccaga gtggatggcg cccttcgccg cgagaatgtg cgtcaggcgt
      781 tcgcactcgc cgcggacgcc ggccgggcgc tccgcgcccg cccggtgctc ctcgtggacg
      841 acgtcgccac gacggggagc acgctcctcg aagccgcatc cgcgctcgat gcggcggccc
      901 cgtcctgggt cctgtcgctc gccttcgctc acggagggct tcccggcgcg ccggaacccg
      961 cgttctcgac gcgggttgcg gctgccccgc cggtgtgata gattctgcga cttggcaaat
     1021 tgggccggct gcgcccggtt tgccataaac ttcgttgacc caactgcttg ctgaccggct
     1081 tcctcccgga gggggacatg gaaaaactgc gactgctgga cgaggcctac ctgccgacgg
     1141 tcaccaagcc gagccggtac acgggtagct tcctccatgc gctccggaag gaccccgcca
     1201 aggcatcggt ccgggtgctc ctctgcttcc cggacctcta cgagatcggg atgtcctatc
     1261 tcgggctgaa gatcctctac cacctgatca atcgccgcga ggacgggctg gcggagctct
     1321 gcttcacgcc gtggggcgac atggagaagc tgatgcgcgc cgaggggatt cccctctact
     1381 cgctggaatc ccgcacgccc gcccgcgcgt tcgacctgat cggcttctcc ctgcaatacg
     1441 agctggccta caccaacgtg ctcacgatga tcgacctcgc cgggatgccg ctccgcacga
     1501 aggaccgccg cgaggacgat ccgatcgtga tcgcgggagg cctctgcacg gtgaacccgg
     1561 agccgatcgc gcccttcatc gacgccttcg tcgtggggga cggcgaagag gtcatgatgg
     1621 agctgtgcga catcgccgcc gagcgccgcc ggacgaagat gccgcgcgcc gaggcgctgc
     1681 gccgcatggc cgcgctcgag ggcgtctacg tgccggcgct cttcgccgag gtgaagaatc
     1741 cctggggctt catcgcccgg gagccgctgc cgggaacgcc ggtcgcgcgg ccgcgcttcc
     1801 gcttcgtgcc gtccctggac ccgagctacg tgccggagaa gccgctcatc tccccgaccg
     1861 agatcacgca cgaccgcctc ggcgtcgagg tgatgcgcgg ctgcacgcgc ggctgccgct
     1921 tctgcctggc cggctacatg aaccgcccgc tgcgcgagaa gcgcgcggac caggtggcga
     1981 aggaagtgca gtgcgggatc gtgcacgccg ggtgggacga ggtctcgctc ctctccctct
     2041 cgaccaccga ctacaagcag ctccccgtgg tgctgcacga gctggaccgc ttcacgaacg
     2101 agctgggcgt gtcggtgtcc ttgccctcga cgcgcgtcgg gacgctcgcc ccgtcggtgg
     2161 ccgacaagat cggccagggg cggaagggca gcatcacgtt cgcccccgag gcgggcacgc
     2221 agcggatgcg cgacgtgatc aacaagggga tcgacgaggc ggagctcgag tactcggtca
     2281 agctggcgcg cgaccagggg tggggcggca tcaagttcta cttcatgatc ggccttccga
     2341 cggaaatgga cgaggacgtg gacgggatcg cggagacgct ccggaagtcg caggagtggg
     2401 cgcgcggcgg gaacaagcgc atgcacttca acgtggggat ctcgccgcac gttccgaagc
     2461 cgcacacgcc gttccagtgg gagatccagg acgatatgga ga
//
LOCUS       DASSLZ010000306         9484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1371584, whole genome shotgun sequence.
ACCESSION   DASSLZ010000306 DASSLZ010000000
VERSION     DASSLZ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371584"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFM00_11555"
     CDS             complement(<1..654)
                     /locus_tag="VFM00_11555"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase (NADP+)"
                     /protein_id="HET7498925.1"
                     /translation="MTDAATRLYAVIGHPVAHSLSPLMQNAAFRAAGRNAAYVALDVP
                     PPRLAEALAGLHAAGFAGLNVTLPHKEEALRLAMSATVSARAAGAANTLRRAEKGWDA
                     DATDGPGLLAWLGALGISVSGARVLVVGAGGASRSVAAALASGGASAITVTNRTPERS
                     AAVAAAARQVAGPGALVDAASWGEAGQAAGAFDFLVRAVSVLEVGSEERRWWERVAPG
                     "
     gene            complement(659..1552)
                     /gene="lgt"
                     /locus_tag="VFM00_11560"
     CDS             complement(659..1552)
                     /gene="lgt"
                     /locus_tag="VFM00_11560"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET7498926.1"
                     /translation="MHPTVVKFGSFAIHSYGLLLAIAFLAAIQLFLARGRARGLPEDK
                     LSTLSLWLLVLAIVGGRLLFVATHWDEYKTDPVAIFRLWEGGLILYGGYALAIAGGIL
                     YVRRAGLSVWRVGDAAAPSMALGVGIGRLGCFMNGCCYGLPTKLPWGITFPPESYSSA
                     VFPHEALHPSQLYMSAAGFLLFFALLALDKRHRFDGWLFWSYIAMDAGLRFLIDFTRY
                     YDETAVLGTFGGLTFNINQLLSVALIAVSLAMLRILAVRAKRHPDTGAGGFEAEHDSG
                     FAPESPAVPTAGEPAGTPPPA"
     gene            complement(1566..2795)
                     /gene="rodA"
                     /locus_tag="VFM00_11565"
     CDS             complement(1566..2795)
                     /gene="rodA"
                     /locus_tag="VFM00_11565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HET7498927.1"
                     /translation="MASLRTPGDVDRPLLFATVTLALIGIAFVFSATSMPSAATEHGL
                     YLKQLIWLAVALGGGALAAAVPYRIYMGRSAWLLYAMGLILLVLTLFIGHVGLGAQRW
                     LGWGPFKFQPSELAKVATVVLLANFLSERRLDLTQLRSLVKPCVVAGVPFLLVLKQPD
                     LGTSVTFLTILFTMLYWAGLPILYLFLLVSPLINVAASFYLPAWIVFAAVLSAVLYRS
                     RLRLMPILLVVAINLVVGIVTPQVWNHLQPYQRQRISTFLDPAADPYGAGYQIIQSKI
                     AIGSGQALGKGFLHGSQKALAFLPEQHTDFIFSVVGEETGFLGAGIVTLLYFLLIYRG
                     IKIAHRSRNRFGSLLAIGLTAIFLYHVLVNVCMTVGLAPVTGLPLPLLSYGGTSLLTS
                     FLQIGLIQNIAMRWREY"
     gene            complement(2797..4623)
                     /gene="mrdA"
                     /locus_tag="VFM00_11570"
     CDS             complement(2797..4623)
                     /gene="mrdA"
                     /locus_tag="VFM00_11570"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET7498928.1"
                     /translation="MPFDPESLTGARYRERHFSIAVAVVFALVLLRLFTMQVLQGPKY
                     RELSEENRIRVEVIVAPRGEIRDRNGVLLADNIPSFTVTLDPYDKTYVDTPARLDDTV
                     RRLGEILQVEPALLREKVRKDGHQSFLPVRLKRNVDRTTVAYIAEHESELPGVDVEFE
                     PLRRYPLGEMASHLLGYVGEVSDKELEDPERSDYLSGDLIGKMGIERQYEKILRGISG
                     KRFVEVNAMGRKAALLGERRPVLPKRGAALTLTIDANLQRAAEQAFQPGARGAAVVMD
                     PRTGEVLALASKPNYDPNEFSTGISQARWALLSEGGNFPLFNRAIQAAYPPGSTLKPF
                     VALAGLMTGAIQPGTTFRETCDGAFQFGRRSFHCWDANGHGTLALRDAVARSCDVYFY
                     QLGIRLGLERLSDFMKELHVSDRAGIDLPQERRGLFPDVAWYDKHYGAGRWSRGLVLN
                     LAIGQGEIALTPIKLAQMTALIANGGTLVRPHLVREVDEDGRPVPLNDSTGMHVAIPA
                     GYAATVRAAMEAVVADPHGTGYAAKVDSVRVAGKTGTAQTHGNDHALFICFAPVENPR
                     IAMAVLVENAGHGSTAAAPIAQKIMQAFFHPTADSVRVVASR"
     gene            complement(4625..5119)
                     /gene="mreD"
                     /locus_tag="VFM00_11575"
     CDS             complement(4625..5119)
                     /gene="mreD"
                     /locus_tag="VFM00_11575"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET7498929.1"
                     /translation="MKSVRDVAVLVVAMILQATFLYRFEIAGIRPDLLVAFVVYLGWM
                     RGPIAGTAGGFLVGLIQDLDAPGPLGLNALSKTIVGFAVAKAGFRVHRANAGVRFAFF
                     LFAMLAHDVVYFAAASLGDFALFLKQMFTIALPSALYTALVVIVILALVENVWKRPLL
                     VDET"
     gene            complement(5116..6027)
                     /gene="mreC"
                     /locus_tag="VFM00_11580"
     CDS             complement(5116..6027)
                     /gene="mreC"
                     /locus_tag="VFM00_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET7498930.1"
                     /translation="MLRYLFTRRTDWTVFVVAVSASLTLMLLGPKSQARTVWFLQHTL
                     LAPAEIVVGWVDRGVAVYWENQRLRQRVARLTIELDETRAERGENERLRRLLHLAERH
                     PYDLVSAHVVGRSLDRLGGSLALDKGEAEGIEANRAILTPDGLVGRIERTSRHAARVL
                     TLLHRDCAVAARIERSRVDGVVRWEYGDRPTLDLLYVSSQEDVKPGDQVLTSGLGGIF
                     PAGVRIGTVERVGLEPNGLTKEIVVDPAVDFRKLEEVLIYVPSARGGSVPSDFYPSAA
                     DSAAADSLRRAAAAAAPAESLAARALR"
     gene            complement(6047..7084)
                     /locus_tag="VFM00_11585"
     CDS             complement(6047..7084)
                     /locus_tag="VFM00_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET7498931.1"
                     /translation="MFLDQVMGVFSNDVAIDLGTANTLVYLRGKGIVLNEPSVVAVDR
                     TTNRVIAVGKEAKSMLGRTPDEIVAVRPLKDGVIADFEKTEDLLREFIQKALRRRTWV
                     RPRIIICVPSGITEVEKRAVQDSAQHAGAREVLLVPEPIAAAIGVGLPVGKPSGNMII
                     DIGGGTTEIAVMALNSIVNQQSIRVGGDEMDEAIVQYVKKAYNLLIGEQTAEQIKIKI
                     GSAFRLEQEEEMEIKGRDLVAGIPKTMKISSVEVREALSEPLQQIVDALMQSLEKTPP
                     ELASDIVDRGIVMTGGGSLLRGIDMLLREATNLPITVAEDPLSCVVLGTGKILDDPTQ
                     YEKVLMSVVRD"
     gene            7379..7900
                     /locus_tag="VFM00_11590"
     CDS             7379..7900
                     /locus_tag="VFM00_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET7498932.1"
                     /translation="MRFLGNIALLFLLAAPAALAAPPTGGSGSDRDRGTGRQPNVDEM
                     KTLGVDRNLASFAGTVLDVNNRPIGGVQVSLFMGGDVAGRAVTGSDGDYELRAPYDPS
                     NDVTVLLWFVAPDRTLMPKELVIQESRASLENGLISKCVPRATLSPGRQFRVYLFDSD
                     SRNKELAESGCLP"
     gene            complement(7943..9265)
                     /locus_tag="VFM00_11595"
     CDS             complement(7943..9265)
                     /locus_tag="VFM00_11595"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7498933.1"
                     /translation="MRRSISAPGSVENEKGCLQGAGRAISKSMYYFYWLPVGTDARVR
                     GTPWATLGLVLLNLAAFGAILVSGGNVEALYAMSFKAADPTIPTALVSLFLHTGFLHL
                     LGNMVFLSVFGPAIESRLGTVRYLLAYLACGWLANLAQAAAILAFTPELAAVPIVGAS
                     GAVSGLMGLFAVRLYFARLRFVSVTVLFLHGTVRPARVTLPALVGIGLWFLLSGVYFV
                     SGAASETAVVAHLGGALFGAFFGFVMGLAPEGRLEHRLARGARYSERGEWYAALGEYE
                     GYLVARPRDPEALAHAARLLRVVHQDGKAGDRFIEAIQAWLKRGAIEPACDAYEEMRR
                     LLGLAAVLPPVEQLRVARGFEELGRPSEASRAYEAYGRSYPERHAAALALLRSADIER
                     RALNNPGRARYIYDELLRRELPADIAEMVRERKQVTERALERLHRGAA"
BASE COUNT         1579 a         3275 c         3312 g         1318 t
ORIGIN      
        1 gccgggcgcc acccgttccc accagcggcg ctcctcggat ccgacctcga gaacggaaac
       61 ggcgcgcacc aggaaatcga acgcgcccgc cgcctggccg gcttcccccc aggacgccgc
      121 gtcgacgaga gcgcccggac ccgcgacctg gcgcgccgcc gcggcgaccg cggcggagcg
      181 ttccggcgtg cgattcgtga cggtgatcgc cgaggctccg cccgaggcga gcgcggcggc
      241 gacgcttcgc gaggcgcctc cggcgcccac gacgaggacc cgcgcgccgg agaccgagat
      301 ccccagcgcg ccgagccagg cgagcaggcc cggaccgtcc gtcgcgtcgg cgtcccagcc
      361 cttctccgcc cggcgcagcg tgttcgcggc gcccgccgct cgtgccgaga cggtcgccga
      421 catcgccagg cgcagcgctt cctccttgtg cggcagcgtc acgttgagtc ccgcgaatcc
      481 cgcggcgtgg agcccggcca gcgcttcggc cagccgcggc ggcggaacgt ccagcgccac
      541 gtacgcggcg ttccgtcccg ccgcgcggaa cgcggcgttc tgcatgagcg gggagagcga
      601 gtgggcgacg gggtgcccga tcacggcgta gagccgcgtc gcggcgtcgg tcacggaatc
      661 aggccggggg aggcgttccc gcgggctcgc ccgcggtcgg gaccgcgggg gattcgggag
      721 cgaagccgga atcgtgttcc gcctcgaagc cgcccgctcc ggtatcggga tgccgcttcg
      781 cccgcacggc gaggatccgc agcatcgcga gcgataccgc gatcagggcc acgctcagga
      841 gctggttgat gttgaacgtg agcccgccga aggtgcccag caccgcggtc tcgtcgtagt
      901 agcgcgtgaa gtcgatcagg aaccggaggc ccgcgtccat cgcgatgtag ctccagaaga
      961 gccagccgtc gaagcggtgc ctcttgtcca gcgcgaggag ggcgaagaag aggagaaagc
     1021 ccgccgccga catgtagagc tgcgaggggt ggagcgcctc gtgcggaaag acggcgctgg
     1081 agtaggactc gggcgggaac gtgatccccc agggaagctt cgtcggaagc ccgtagcagc
     1141 agccgttcat gaagcagccc aggcgtccga tgccgacgcc gagcgccatg ctcggcgcgg
     1201 cggcgtcccc cacccgccag accgagagtc cggcgcgtcg gacgtagagg atcccaccgg
     1261 cgatcgcgag ggcgtagccg ccgtagagga tcagcccccc ctcccacaat cggaagatcg
     1321 cgaccgggtc ggtcttgtac tcgtcccagt gcgtcgccac gaagaggagc ctgcccccca
     1381 cgatggcgag gaccaggagc cagagcgaca gcgtgctcag cttgtcttcg gggagccccc
     1441 gcgcgcggcc gcgcgcgagg aagagctgga tcgcggccag gaacgcgatc gcaaggagca
     1501 gcccgtagct gtggatcgcg aacgagccga acttcaccac ggttggatgc acggctctcc
     1561 ccgggtcagt actcgcgcca ccgcatggcg atgttctgga tcaggccgat ctgcaggaag
     1621 ctggtcagga gcgaggtgcc gccatagctc aggagcggaa ggggcagccc cgtgacgggg
     1681 gcgagtccca ccgtcatgca gacgttcacc aggacatggt acaaaaagat ggcggtcaat
     1741 ccaatggcga ggaggctgcc gaaccggttc cgggatcggt gggcgatctt gatcccccgg
     1801 tagatgagga ggaagtagag gagcgtcacg atccccgccc ccaaaaaccc cgtctcctcc
     1861 cccaccaccg agaagatgaa gtcggtgtgc tgttcgggga ggaaggcgag cgccttctgg
     1921 ctgccgtgca ggaacccctt gcccagcgcc tgccccgacc cgatcgcgat cttcgactgg
     1981 atgatctgat agcccgcgcc gtagggatcg gccgcgggat cgaggaaggt ggagatgcgc
     2041 tggcgctggt agggctggag gtggttccag acctgcggcg tcacgatccc cacgacgagg
     2101 ttgatggcca cgacgaggag gatcggcatg aggcgcagcc gcgagcggta gaggacggcc
     2161 gagagcacgg cggcgaagac gatccacgcc ggaaggtaga aggacgccgc cacgttgatc
     2221 agcgggctca cgaggaggaa caggtagagg atcgggagac ccgcccagta gagcatcgtg
     2281 aagaggatcg tgaggaaggt gacggaggtg cccaggtcgg gctgcttcag cacgaggagg
     2341 aacggcactc cggccaccac gcacggcttc accagcgagc ggagctgcgt caggtccagg
     2401 cgccgctcgg agaggaaatt cgcgagcagc accaccgtcg ccactttcgc gagctcggag
     2461 ggctggaact tgaacggccc ccagcccagc catcgctgcg cccccaaccc cacgtgcccg
     2521 atgaagaggg tgagcacgag caggatgagc cccatggcgt agagcagcca ggcgctccgg
     2581 cccatgtaga tccggtaggg cacggccgcc gcgagcgcgc ccccgccgag cgcgaccgcg
     2641 agccagatga gctgcttcag atagagcccg tgctcggtcg cggccgaggg catcgaggtc
     2701 gcgctgaaga cgaacgcgat cccgatcagc gccagcgtga cggtggcgaa gaggagcgga
     2761 cggtcgacgt cgccgggcgt gcgcaggctg gccatgtcag cggctcgcca cgacccgcac
     2821 actgtcggca gtgggatgga agaaggcctg catgatcttc tgcgcgatcg gcgcggccgc
     2881 ggtgctcccg tggccggcgt tctcgaccag cacggccatc gcgatcctcg gattctcgac
     2941 cggcgcgaag cagatgaaga gcgcgtggtc gttcccgtgg gtctgcgcgg tgccggtctt
     3001 ccccgccacg cgcaccgaat cgaccttggc ggcgtagccg gtcccgtgcg gatcggcgac
     3061 gacggcctcc atcgcggcgc gcaccgtggc ggcgtagccg gccggaatgg cgacgtgcat
     3121 cccggtggaa tcgttcagcg ggaccgggcg cccgtcctcg tccacctcgc gaaccaggtg
     3181 ggggcggacc agcgtgccgc cgttggcgat cagcgccgtc atctgcgcca gcttgatcgg
     3241 cgtgagcgcg atctcaccct gcccgatcgc gaggttcagg acgagcccgc ggctccagcg
     3301 gcccgcgccg tagtgcttgt cgtaccacgc cacgtccgga aagagcccgc gccgctcttg
     3361 cgggaggtcg atccccgcgc gatcggagac gtgaagctcc ttcatgaagt cggagaggcg
     3421 ctccaggccg agacggatgc ccagttggta gaagtagacg tcgcaggagc gcgcgacggc
     3481 gtcgcgcagc gcgagcgtgc cgtggccgtt ggcgtcccaa cagtggaagg agcggcggcc
     3541 gaactggaac gcgccgtcgc aggtctcacg gaacgtggtg ccgggctgaa tggcgccggt
     3601 catgagtcct gccagcgcaa cgaagggctt caaggtggag cccggcggat acgccgcctg
     3661 gatcgcgcgg ttgaagagcg ggaagttgcc cccctcgctc agcagcgccc agcgcgcctg
     3721 gctgatcccg gtcgagaatt cgttggggtc atagttgggc ttgctcgcca gcgccaggac
     3781 ctcccccgtg cgcggatcca tcacgaccgc ggcgcctcgc gcgccgggct ggaacgcctg
     3841 ctccgccgcg cgctggagat tggcgtcgat ggtgagcgtg agcgccgcgc cgcgcttcgg
     3901 cagcaccggg cggcgctcgc ccaggagcgc ggccttccgt cccatggcgt tcacctccac
     3961 gaagcgcttc cccgagatgc cgcgcaggat cttctcgtac tggcgctcga tgcccatctt
     4021 gccgatcagg tccccgctca gatagtcgga gcgctcggga tcctccagct ccttgtccga
     4081 gacttcgccg acgtacccga ggaggtgcga ggccatctcc ccgagcgggt agcggcggag
     4141 cggctcgaac tccacgtcca cgcccggaag ctccgactcg tgttcggcaa tgtaggcgac
     4201 ggtggtccgg tccacgttcc gcttgaggcg caccggcagg aacgactggt gtccgtcctt
     4261 gcgcaccttc tcgcgcagga gcgcgggctc gacctggagg atctctccca ggcggcgcac
     4321 ggtatcgtcc agacgcgcgg gcgtgtccac gtaggtcttg tcgtagggat cgagggtgac
     4381 ggtgaaggac gggatgttgt cggccaggag gacgccgttg cggtcgcgga tctccccgcg
     4441 cggcgccacg atcacctcga cgcggatgcg gttctcctcg gagagctcgc ggtacttggg
     4501 cccctggagc acctgcatcg tgaacagtcg gagcagcacc agcgcgaaca ccaccgccac
     4561 ggcgatggag aaatggcgct cccggtagcg cgccccggtc agcgattcgg ggtcgaacgg
     4621 cacgctacgt ctcgtcgacc aggagggggc gcttccagac gttctcgacc agcgcgagga
     4681 tgacgatgac gaccagcgcc gtgtagagcg ccgacgggag cgcgatcgtg aacatctgct
     4741 tcaggaagag cgcgaagtcg cccagcgagg ccgccgcgaa gtagacgacg tcgtgcgcca
     4801 gcatggcgaa gaggaagaac gcgaaccgca cgcccgcgtt ggcgcggtgg acccggaacc
     4861 cggctttggc cacggcgaat cccacgatgg tcttggagag cgcgttcagg ccgagcgggc
     4921 cgggcgcgtc caggtcctgg atcaatccga ccaggaatcc cccggcggtg ccggcgatcg
     4981 gcccccgcat ccatcccaga taaaccacga acgcgacgag gaggtccggg cggatccccg
     5041 cgatctcgaa gcggtagagg aacgtcgcct ggaggatcat ggcgaccacg aggacggcga
     5101 cgtcgcggac gctcttcatc ggagcgccct ggcggcgagc gattcggccg gcgccgcggc
     5161 cgccgcggcc cggcgcagcg agtcggcggc ggcgctgtcc gcggccgacg ggtagaagtc
     5221 ggagggcacg gatccgccgc gcgccgaagg cacgtagatc agcacctcct cgagcttccg
     5281 gaaatcgacc gccggatcca cgacgatctc cttcgtgagt ccgttcggct cgagtcccac
     5341 gcgctccacg gtgccgatcc gcacgcccgc cgggaagatc ccgcccaggc cggaggtcag
     5401 gacctggtct cccggcttca cgtcctcctg ggacgagacg tagaggagat ccagcgtcgg
     5461 acggtccccg tattcccagc ggacgacgcc gtccacgcgc gagcgctcga tccgcgcggc
     5521 cacggcgcag tcgcgatgga gcagcgtgag cacgcgggcg gcgtgccgcg acgtccgctc
     5581 gatacgcccc accagtccgt cgggggtgag gatcgcgcgg ttcgcctcga tcccctcggc
     5641 ctctcccttg tcgagggcca ggcttccgcc gaggcggtcg agcgagcggc ccaccacgtg
     5701 cgcggagacg aggtcgtagg ggtggcgctc ggccaggtgc agcaggcggc gcaggcgctc
     5761 gttctcgccc cgctcggccc gggtctcgtc cagctcgatg gtgaggcgcg ccacgcgctg
     5821 gcgcaggcgc tggttctccc agtagaccgc gacgccgcgg tccacccagc cgacgacgat
     5881 ctcagcgggc gctaaaaggg tgtgctggag gaaccagacg gtccgggcct gagacttcgg
     5941 cccgagcagc atgagggtca gcgaagcgga gacggcgacg acgaagacgg tccagtcagt
     6001 ccggcgtgtg aacaggtacc gcagcatcgg gactcacgcc cgaggctcag tcacgcacca
     6061 ccgacatgag caccttctcg tattgagtgg ggtcgtcgag gatcttgccg gtccccagca
     6121 ccacgcagga gagcgggtct tccgcgaccg tgatcggaag attcgtggct tcgcgaagga
     6181 gcatgtcgat gccgcgcagg agggagccgc ctcccgtcat gacgatgccg cggtcgacga
     6241 tgtccgaggc cagctcgggc ggcgtcttct ccagggactg catcagggcg tcgacgatct
     6301 gctgcagcgg ctcgctcagg gcctcgcgca cctcgacgga cgagatcttc atcgtcttcg
     6361 ggatgccggc caccaggtcg cgccccttga tctccatctc ttcctcctgc tcgaggcgga
     6421 aggcggagcc gatcttgatc ttgatctgtt cggcggtctg ctcgccgatc agcaggttgt
     6481 acgccttctt cacgtactgg acgatcgctt cgtccatctc gtcgccgccg acgcggatcg
     6541 actgctggtt cacgatcgag ttcagcgcca tgaccgcgat ctcggtcgtg ccgccgccga
     6601 tgtcgatgat catgttcccc gacggcttgc ccaccggcag acccacgccg atggccgcgg
     6661 cgatcggctc gggaaccagg agcacctcgc gcgcacccgc gtgctgcgcc gagtcctgca
     6721 cggcgcgctt ctccacctcg gtgatgcccg agggaacgca gatgatgatg cgggggcgaa
     6781 cccacgtgcg gcggcggagg gccttctgga tgaattcgcg gaggaggtct tccgtcttct
     6841 cgaagtcggc gatgacgccg tccttgagcg gccgcaccgc gacgatctcg tcgggcgtgc
     6901 gacccagcat gctcttcgct tccttgccca ccgcgatcac gcggttggtg gtgcggtcca
     6961 ccgccacgac ggagggttcg ttcaggacga tccctttgcc acggaggtag acgagcgtat
     7021 tcgcggtgcc caggtcgatc gcgacgtcgt tcgagaacac acccatcacc tggtcgagaa
     7081 acatccgtgc ctcccgaacg cattcgtgcg aggtggcagg ctgggccgcc gccgcttccc
     7141 gtcgtcagcc acccccccag aaactcccaa gtcggcgacc tcgttggcga tccgcgcggg
     7201 cacagcaagc attgaggtgc gaagcgaacg caacgtatca aacgcactac ggaggcgcaa
     7261 gtccggattc tccacggaag ccgcgttgac aggctgggat acgggtgcta gcgtccgacg
     7321 agggacgtgt ggggcccgcg acgtgacagg gagggtatcg gccatggcgg gatcgaactt
     7381 gaggttcctc ggaaacatcg cccttctttt cctgctcgcg gctcctgccg ccctcgccgc
     7441 cccgcccacg ggcgggtccg ggagcgaccg ggatcgcggc acggggcgcc agcccaacgt
     7501 cgacgagatg aagaccctcg gcgtcgaccg caacctcgcc tccttcgccg gaacggtcct
     7561 cgatgtgaac aaccggccga tcggcggggt ccaggtctcg ctcttcatgg gcggcgacgt
     7621 cgccggccgc gccgtgaccg ggtccgacgg agactacgag ctccgcgccc cgtacgaccc
     7681 ctccaatgac gtcacggtcc tcctctggtt cgtcgcgccc gaccggacac tgatgccgaa
     7741 ggagctggtc atccaggagt cgagggccag cctggagaac ggcctcatct ccaagtgcgt
     7801 gccgcgggcg actctctcgc ccgggcgcca gttccgggtg tacctgttcg acagcgacag
     7861 ccggaacaag gagttggccg agtcgggctg cctgccctag gcgggctttc gagtccggtc
     7921 ggcggggccg cccgagccgc ggtcaggcgg ccccgcgatg caggcgctcc agcgcccgct
     7981 ccgtcacctg ctttcgctcc cgcaccatct cggcgatatc cgccggcagc tcccgccgca
     8041 acagctcgtc gtagatgtag cgcgcccgcc ccgggttgtt gagggcgcgg cgctcgatgt
     8101 ccgcgctccg gaggagggcc agggcggcgg cgtgccgctc ggggtagctc cggccatagg
     8161 cttcgtaggc gcggctcgcc tcgctggggc gtcccagctc ctcgaagccc cgcgccaccc
     8221 ggagctgctc cacgggcggc agcaccgcgg ccaggccgag tagccggcgc atctcttcgt
     8281 aggcgtcgca cgccggctcg atcgcgccgc gcttcagcca ggcctggatc gcctcgatga
     8341 accgatcccc cgccttcccg tcctgatgca ccacgcgcag aaggcgcgcc gcgtgcgcca
     8401 gcgcttcggg gtcccgcggg cgcgccacga ggtagccctc gtactcgccg agggcggcgt
     8461 accactcccc ccgctcggag tagcgggcgc ctcgcgccag gcggtgctcc agccgcccct
     8521 cgggggccag ccccatcacg aagccgaaga aggcgccgaa gagcgctccg cccaggtgcg
     8581 ccacgacggc ggtctccgag gcggcccccg agacgaaata gactccggag agcaggaacc
     8641 agagcccgat ccccaccagt gccggcagcg tcacgcgggc cggacgcacc gtgccgtgca
     8701 ggaagagaac cgtcaccgag acgaaccgca ggcgcgcgaa gtagagccgc accgcgaaga
     8761 ggcccatcag ccccgacacg gcccccgagg cgcccacgat cggaacggcc gccagctctg
     8821 gggtgaaggc gaggatcgcc gcggcctggg ccaggttggc gagccagccg caggccaggt
     8881 aggccagcag gtaccggacc gtccccagcc tcgattcgat cgcggggccg aagaccgaca
     8941 ggaagaccat gttcccgagg aggtgcagga atccggtgtg caggaagagc gagaccaggg
     9001 ccgtcgggat ggtcgggtcg gcggccttga aggacatggc gtagagcgcc tcgacgttcc
     9061 ccccgctgac gaggatcgcg ccgaaggccg ccagattcag gagcaccagc ccgagcgtcg
     9121 cccaaggggt cccccggacc cgggcgtcgg tcccgaccgg cagccagtag aagtagtaca
     9181 tcgacttaga aatagcacga ccagcccctt gaaggcagcc cttctcgttc tctacactac
     9241 ccggcgccga aatcgatcgc cgcacgcccg cgcacgccgg cccccggcac gccgccacta
     9301 ccaaaggaag gccatggaaa gcgtcaccca taccggtatg gacatgggca ccgagctcca
     9361 gaaggagctg gacccgctgg aaaacgcccg gctccagttc gaagaggccg ccgcccggct
     9421 caagctggac ccctccttcc tccagatcat caaggagccg cgccgcgcca ccatcgtgaa
     9481 gctc
//
LOCUS       DASSLZ010000307         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1736414, whole genome shotgun sequence.
ACCESSION   DASSLZ010000307 DASSLZ010000000
VERSION     DASSLZ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736414"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2020
                     /locus_tag="VFM00_11600"
     CDS             <1..2020
                     /locus_tag="VFM00_11600"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET7498934.1"
                     /translation="STGSTFRLSLDDRDRDGEMDVGIRPLSDPRLELFGAYSLHRDQR
                     WDDGDLAGGVAIRPVPGLFASARWERDDRLQVTLGVAVGRAAFRASPRYRGGDRAQTA
                     YAVRLSSPERGFDLDRVMTPKRRTLALDLRGHMVYQSYRYFDQDARPLREVTERIQFA
                     ADDPTVGGVAIKLSGFEGNLSMAWEIREKLMALRRAGKRITVVCDNLGAPTLYVASAA
                     DRIVMDPRGSMILPGVAASRTYYHDLLGKLGIGFEEWRFYIYKSAMETFSRDRMSEAD
                     REQYGAIVRATYDELARAFTSSGRATRAEFDRVVNEEPYIPASRLLELHWVDALGRWE
                     DLERAEVGAPAMKPVGYGALRELRWQPRERWGNDPTIAVVYLVGDCAMDEGIRARSSS
                     EALRGYRKDRRVRGVVLRVDSPGGDPLASDVVAEQTRKLRQVKKPAVVSQGRVAASGG
                     YWISMDGDAILTSPFTLTGSIGVIGGWAWNEGFGKKTGLTSDHVQEGKSADLLGGLRI
                     PFLGATLPERNLDEGERRLIRRSFDELYTDFTGKVASARGLTTERVRQIAEGHVYGGA
                     AAIPLKLADREGTLDDAIAEAKRRAGIRAGERVRVVEYPARPLFRLPSFLRPPGIGAG
                     SWAPAAAESPVPGFPLEARALQSILDRPGRPLLVTPGPLLPDEPEPVR"
     gene            2078..>2582
                     /locus_tag="VFM00_11605"
     CDS             2078..>2582
                     /locus_tag="VFM00_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498935.1"
                     /translation="MSNATIGSIYLLVAGLVFLLGFVILREAPRERLNRATALMLFFA
                     GLGSVLGAIGFILESLSAARAGSNDLLRSFNYLWELFFPSLLYFACVFPSENRLIRRV
                     PFAVGWIFAPHVAHLCFMVLASQGALWGRVAAAMAKSSASGVFTYLRIPFELIFRFHQ
                     LLFSMVNL"
BASE COUNT          336 a          917 c          908 g          421 t
ORIGIN      
        1 cagcaccggc agcacgtttc gcctctcgct ggacgaccgg gaccgggacg gagaaatgga
       61 cgtgggcatc cgtccgctct ccgatccgcg gctggagttg ttcggcgcct attcgctcca
      121 tcgcgatcag cgctgggacg acggggacct ggcgggaggc gtcgcgatcc gccccgtccc
      181 gggactcttc gcctccgccc gctgggagcg cgacgatcgg ctgcaggtga cgctcggggt
      241 ggccgtcggg cgcgccgcgt tccgggcttc gccccgctat cgcggcggcg atcgcgcgca
      301 gaccgcctat gcggtccggc tgagctcgcc cgagcgtggc ttcgatctcg accgcgtcat
      361 gactccgaag cgccgcaccc tggctctcga tctgcgcggc cacatggtct accagtccta
      421 ccgctacttc gaccaggacg cccgtcccct gcgggaggtg accgagcgga tccagttcgc
      481 tgccgacgac cccaccgtgg gcggggtggc gatcaagctc tccggcttcg aggggaacct
      541 ctcgatggcc tgggagatcc gcgagaagct gatggcgctg cgccgcgccg gaaagcgcat
      601 caccgtggtc tgcgacaatc tcggcgcccc gaccctgtat gtcgccagcg cggccgaccg
      661 gatcgtcatg gacccgcggg gctcgatgat ccttcccggc gtggccgcgt cgcgcaccta
      721 ctaccacgat ctcctgggca agctgggcat cggcttcgag gaatggcgct tctacatcta
      781 taagtccgcg atggagacct tctcgcggga ccgcatgtcc gaggcggacc gcgagcagta
      841 cggggccatc gtccgcgcca cctacgacga gctggcgcgg gcgttcacgt ccagcggccg
      901 cgccacgcgc gcggagttcg atcgcgtcgt gaacgaggag ccgtacatcc ccgcgtcgcg
      961 cctgctggag ctgcactggg tggacgcgct ggggcgctgg gaggacctcg aacgggccga
     1021 ggtcggcgcg cccgcgatga aaccggtcgg ctacggcgcc ctgcgcgagc tccgatggca
     1081 gccccgggag cggtggggca acgatccgac gatcgcggtc gtctatctcg tgggcgactg
     1141 cgccatggac gaggggatcc gcgcccggtc gagctccgag gcgctccgcg gctaccgcaa
     1201 ggaccgccgc gtccgcggcg tcgtgctgcg tgtggattcg ccgggaggcg acccgctggc
     1261 ctccgatgtc gtggccgagc agacgcggaa gctccgccag gtgaagaagc ccgcggtggt
     1321 gagccagggg cgcgtggcgg ccagcggcgg ctactggatc agcatggacg gggacgcgat
     1381 cctgacctcc cccttcacgc tgacgggatc gatcggggtg atcggcggat gggcctggaa
     1441 cgagggattc gggaagaaga ccgggctcac gtcggaccac gtgcaggagg ggaagagcgc
     1501 cgatctgctg ggtgggctcc gcatcccctt cctgggcgcg acgttgccgg aacgaaacct
     1561 ggacgaggga gaacgccgtc ttatccgtag gtcgttcgac gagctctaca ccgacttcac
     1621 cggcaaggtg gcgtcggccc gggggctcac gacggagcgg gtgcggcaga tcgccgaggg
     1681 gcatgtctac ggcggcgccg ccgcgatccc gttgaagctg gccgaccgcg agggcaccct
     1741 ggacgacgcg atcgccgagg cgaagcgccg ggcggggatc cgggccggcg agcgggtgcg
     1801 cgtggtggaa tatcccgcgc ggccgctctt ccggcttccg tcgttcctga gacctccggg
     1861 gattggcgcc gggagttggg cgcctgcggc cgccgagtcg ccggtcccgg gcttcccgct
     1921 cgaggcgcgg gccctgcagt cgattctcga ccggcccggc cgtccgctgc tcgtcacccc
     1981 ggggccgctc ctccccgacg agcccgagcc ggtccgctag ggtcggcccg cccgcgcgcg
     2041 cccggccggc gcgcaaccgg agtccgaagc gcagctcatg tccaacgcca ccatcggctc
     2101 catctacctg ctggtcgccg ggctcgtctt cctcctcggc ttcgtgatcc tgcgcgaggc
     2161 cccccgggag cgactgaacc gggccacggc cctgatgctg ttcttcgcgg ggctcggctc
     2221 ggtgctcggc gcgatcggct tcattctgga gtccctctcg gcggcccgcg ccggcagcaa
     2281 cgacctgctg cgctccttca actatctctg ggagctcttc ttcccgtcgc tcctctattt
     2341 cgcctgcgtc ttcccctcgg agaaccgcct gatccggcgc gtccccttcg cggtcggatg
     2401 gatcttcgcg ccccacgtgg cgcacctctg cttcatggtg ctcgcctcgc agggcgcgct
     2461 gtgggggcgg gtcgcggcgg cgatggcgaa gagctccgcc tccggcgtct tcacctatct
     2521 ccgcatcccg ttcgagctga tcttccgctt ccatcagctc ctcttctcga tggtgaacct
     2581 ct
//
LOCUS       DASSLZ010000308         4080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_111386, whole genome shotgun sequence.
ACCESSION   DASSLZ010000308 DASSLZ010000000
VERSION     DASSLZ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111386"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFM00_11610"
     CDS             complement(<1..245)
                     /locus_tag="VFM00_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498936.1"
                     /translation="MFGLELHPAVIHYPIALGTIGSLALLAYAILRKDWLRWFGPILL
                     TLALAGAGAAYFSGESASDRAEHAGVPDKEIEQHESIA"
     gene            complement(332..3292)
                     /locus_tag="VFM00_11615"
     CDS             complement(332..3292)
                     /locus_tag="VFM00_11615"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HET7498937.1"
                     /translation="MDPQRELQRDLAPLLAGEVRTDPITLALFATAACIFRRKPLAVV
                     SPKSTADVAAVMAFASEHGIPVTPRGGGSSLAGAALGPGIILDFSAHMHHVLEVDPGQ
                     RRVRVEPGAIHTRVQRAAAPYGLRLGPDPSSGDFCTIGGNIGTNAAGAHTLRHGTTKD
                     HLEALTVVLHDGSVVRLGEGAAGEEPAVWTRTAEHLTQILRAGAPRFLPERPRANKNS
                     CGYDLWGAWNPGDQVSSIEPRFDPIRLLCGSEGTLGIVTEATMRLVPKPSATAVALLY
                     FASWHEATEAVLEARRAGASAIEAMDHTFLEFVKKDRPDLRDLIPDRFEAGLLVEFEG
                     AADEEARTGLAAIEEWVAARPGKVLDFRAARDAVERAALWSVRRAALPLVYRAEPVER
                     PMNFIDDTAVPAEALGEYIGGLRSIFAKHRTRYVVFGHAGNGNVHVVPLLDPHEATFV
                     PRMAAMAEDAFELTWRLGGTITGEHGDGVLRAPYLRRQYPESYDVMAEVKHALDPRGI
                     LNPGSVISDASTFPGDSLRYTNEYVVTGTVFDEPDYRAMIEHCHGCGTCRDYCPVGST
                     TLIEPHAARAKASLLMELIRGALHPDMLTGKPFKAVMDSCFNCKLCLSECPSQVDIPG
                     LAVAARRAFVEKKGMPLRNWVLGNSSKVAQVASVAPGLVNAAIGNAAERAARESVGKI
                     AGKLDLPRFRRSFRVGDEGSKRALALPLVPAETAAGAGADPGGGHAPGANHHARLGPL
                     PPRHVPITKKVAYFAGCFARFHDPEGEAAATVKVLEANGVEVVVPDQRCCGIALITMG
                     AEAKIKADAERNVRTLLPLVDRGFTVVASAPSCGLALIEDYPRILGTEEARRLADHTI
                     DVHQYLCALHERGELNTSFQEVPLTVVYHNACHSVAQGMTEEPVRLLKMIPGVTVRPI
                     EDSCCGIAGTYGMRAENYDRAKAIGEPLMHQMDRANAEVLLTSCGTCNIQIANGLKRP
                     VTHTMAILRRAYGV"
     gene            complement(3317..3985)
                     /locus_tag="VFM00_11620"
     CDS             complement(3317..3985)
                     /locus_tag="VFM00_11620"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HET7498938.1"
                     /translation="MPSARKFLAEFLGTFALVLFGAGAILQQQMTQTVGTTGIAVAHG
                     LAIMVSIYALGHISGGHFNPAISFAMFLTRRLSAGMTLLYWVAQLAGAVVAAWLLAMA
                     YHNGAADAHLGAPVADPSIRPSLAALLEGVAAFMLVLVVFGSAVDPRAPKGFYGLAIG
                     LTLTANILMGGVLTGMAFNPARAFGPALVAGFWQDQWVYWVGPLLGGGVAGILYDRFF
                     LERA"
BASE COUNT          613 a         1438 c         1439 g          590 t
ORIGIN      
        1 gcgatcgact cgtgctgctc gatctccttg tcgggcacgc ccgcgtgctc cgcccggtcc
       61 gaggccgact cgccgctgaa gtacgcggcg cccgcgccgg ccagggccag ggtcaacagg
      121 atgggtccga accaccggag ccagtccttg cggagaatgg cgtaggcgag gagggcgagc
      181 gagccgatcg tgccgagcgc gatcggatag tggatcacgg ccgggtgcag ctccagtccg
      241 aacacggagg ctcctttcgc tcgggcgcgt cgaatcgggc tatggctcgg gtgaacgcac
      301 gccggcgtcg actcgtcggc gacgccgcgg gctaaacgcc gtaggcgcgg cgcaggatcg
      361 ccatggtatg ggtgaccggg cgcttcagtc cattcgcgat ctggatgttg caggtgccgc
      421 acgaggtgag cagcacctcg gcgttcgcgc ggtccatctg gtgcatgagc ggctcgccga
      481 tcgctttcgc gcgatcgtag ttctcggcgc gcatgccgta ggtgccggcg atgccgcagc
      541 aggagtcctc gatcggccgc accgtgacgc cgggaatcat cttcaacagt cgcaccggct
      601 cctcggtcat cccctgcgct acggagtggc aggcgttgtg gtagacgacg gtgagcggca
      661 cctcctggaa cgaggtgttc agctcgccgc gctcgtgcag ggcgcagagg tactggtgca
      721 cgtcgatggt gtggtcggcc agccgccgcg cctcctcggt gcccaggatg cgcggatagt
      781 cctcgatcag cgcgagcccg cacgagggcg cgctcgcgac cacggtgaac ccgcggtcca
      841 ccagcggcag cagcgtccgg acgttccgct cggcgtcggc cttgatcttg gcctcggcgc
      901 ccatcgtgat gagcgcgatg ccgcagcagc gctggtcggg cactaccacc tcgacgccgt
      961 tcgcctccag caccttcacg gtggccgcgg cttccccctc gggatcgtgg aagcgcgcga
     1021 agcagccggc gaaataggcc accttcttcg tgatcggcac gtgccgcggc ggcaacgggc
     1081 ccagccgggc gtggtggttc gcgccgggag cgtggccccc tcccggatcc gcgccagcgc
     1141 cagccgcggt ctccgccggg acgagcggaa gcgcgagcgc ccgtttcgat ccctcgtccc
     1201 ccacccggaa cgacctccgg aagcgcggca gatcgagctt ccccgcgatc ttccccaccg
     1261 actcccgggc cgcgcgctcg gcggcattgc cgatcgccgc gttcaccagc cccggagcga
     1321 ccgacgcgac ctgtgccacc ttggacgaat tcccgagcac ccagttccgg agcggcatcc
     1381 ccttcttctc cacgaacgcg cggcgcgccg ctaccgccag ccccgggatg tccacctggc
     1441 tcgggcattc gctcaggcag agcttgcagt tgaagcagga gtccatgacc gccttgaagg
     1501 gcttgccggt cagcatgtcg ggatggagcg cgccgcggat cagctccatc agcagcgacg
     1561 ccttggcgcg cgcggcgtgc ggctcgatca gcgtcgtgct cccgaccggg cagtagtcgc
     1621 ggcaggtgcc gcagccgtgg cagtgctcga tcatggcgcg gtagtcgggc tcgtcgaaga
     1681 cggtgccggt cacgacgtac tcgttggtgt agcggagcga gtcgcccggg aacgtcgaag
     1741 cgtccgagat caccgagccg ggattcagga tgccgcgggg gtcgagcgca tgcttcacct
     1801 cggccatcac gtcgtacgat tccgggtact gccggcggag atagggcgcg cgcagaacgc
     1861 cgtcgccgtg ctctccggtg atggtccccc cgaggcgcca cgtcagctcg aaggcgtcct
     1921 ccgccatcgc ggccatgcgc ggcacgaacg tggcttcgtg cgggtccagg agcggcacca
     1981 cgtgcacgtt cccgttcccc gcgtgaccga acacgacgta gcgcgtccgg tgcttggcga
     2041 agatggagcg caggccgccg atgtactcgc ccagcgcctc ggcgggcacg gccgtgtcgt
     2101 cgatgaagtt catcggccgc tcgaccggct cggcccggta gaccagcggc agcgcggcgc
     2161 ggcgcacgct ccagagggcg gcccgctcga ccgcgtcgcg cgcggcgcgg aaatcgagca
     2221 ccttccccgg gcgcgccgcg acccactcct cgatcgcggc cagcccggtc cgcgcttcct
     2281 cgtcggccgc gccctcgaac tccaccaaca gtcccgcctc gaaccggtcg gggatcagat
     2341 cgcgcaggtc ggggcggtcc ttcttcacga actcgaggaa cgtgtggtcc atcgcctcga
     2401 tcgccgaggc ccccgcgcgc cgcgcctcga gcaccgcctc ggtcgcctcg tgccacgagg
     2461 cgaagtagag gagcgcgacc gcggtcgccg acggcttcgg aacgaggcgc atggtcgctt
     2521 cggtcacgat gccgagcgta ccctcggatc cgcacaacag ccggatcgga tcgaagcgcg
     2581 gctcgatgga ggacacctgg tcgcccggat tccacgcgcc ccagaggtcg tagccgcagg
     2641 agttcttgtt cgcgcgcggg cgctccggta agaaccgcgg cgctcccgct cgaaggatct
     2701 gggtcaggtg ttcggccgtg cgggtccaga ccgcgggctc ctcgcccgcc gcgccctccc
     2761 ccaggcggac gacgctgccg tcgtggagca ccacggtgag cgcttcgaga tggtctttcg
     2821 tggtcccatg gcggagcgta tgggcgccgg cggcgttcgt cccgatgttc ccgccgatgg
     2881 tgcagaagtc cccggacgag ggatcgggac cgaggcgcag gccgtagggc gcggccgcgc
     2941 gctgcacgcg cgtgtggatc gcgcccggct ccacgcgcac ccgccgctgg ccgggatcca
     3001 cctcgagcac gtggtgcatg tgcgccgaga agtcgaggat gatcccgggg cccagggccg
     3061 cgccggccag gctggatccg ccgccgcgtg gcgtgaccgg aatgccgtgc tcggaggcga
     3121 aggccatgac ggccgcgacg tccgcggtcg atttcggcga caccacggcg agcggcttcc
     3181 tgcggaagat gcaggccgcc gtcgcgaaga gggcgagggt gatcgggtcg gtgcgaacct
     3241 cgcccgcgag aagcggagcg aggtcgcgct gcagttcacg ctgcgggtcc atcttcaccc
     3301 cgcgcgcggg tacggctcag gcgcgctcga gaaagaaacg atcgtagagg atgccggcga
     3361 ctccgccccc caggagcggc cccacccagt atacccactg gtcctgccag aagcccgcca
     3421 ccagggcggg cccgaaagcg cgcgccggat tgaaggccat accggttaga accccgccca
     3481 tgaggatgtt cgccgtgagc gtgagcccga tcgccaggcc gtagaagccc ttgggcgccc
     3541 gggggtccac ggcggacccg aagaccacga gcaccagcat gaaggcggcc accccctcga
     3601 ggagcgcggc cagggagggg cgtatcgacg gatcggccac cggggccccg agatgcgcgt
     3661 cggccgcccc gttgtggtag gccatggcca gcagccaggc cgcgacgacg gctccggcca
     3721 gctgggccac ccagtagagg agcgtcatgc ccgccgagag gcggcgcgtg aggaacatgg
     3781 cgaagctgat cgcgggattg aagtggcctc cggaaatatg gcccagcgcg tagatgctga
     3841 ccatgatcgc gaggccgtgc gccaccgcga tcccggtcgt gcccaccgtc tgggtcatct
     3901 gctgctggag aatcgctccc gccccgaaca ggacgagagc gaaggtcccg agaaactccg
     3961 ccaggaattt cctcgcgctg ggcatcagag ccctcccacc gttttggcgt cgagccgctt
     4021 ggccagctcg accaggagcc gcacgccggc gtcgtagtcc ttgcggtcca tgatcgtcgc
//
LOCUS       DASSLZ010000309         9986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_371074, whole genome shotgun sequence.
ACCESSION   DASSLZ010000309 DASSLZ010000000
VERSION     DASSLZ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9986
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371074"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFM00_11625"
     CDS             complement(<1..516)
                     /locus_tag="VFM00_11625"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HET7498939.1"
                     /translation="MQTVVRSTLAPLTDFRSAEAGLFVAQLDDQLRLVKEATRGLTPD
                     DLQWQPAAGMNTIGMLLAHLAVVEVWWTKFALGGEPAADVDDVLGIGGDDDGLPLPEG
                     APAYAPLNGKDLAFYDDLLDRGRGYLKSMAKDRVPEDLDREIPRERPDGSIRLLNVRW
                     YYYHLLEHFAGH"
     gene            complement(542..1114)
                     /locus_tag="VFM00_11630"
     CDS             complement(542..1114)
                     /locus_tag="VFM00_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7498940.1"
                     /translation="MDRIHRAALLVTIAVAAVSCSKNTGQSGSAPADSAAQAEAAMTP
                     VQRGQYLVTVLGCHDCHTPGTLYGAPDMNRQLSGSELGWQGPWGVSYPRNLTPDSTGL
                     AAWSDEEILRAIQNGVKRDGSPIAPPMPWPDFAHLTPADAKAVVAYLRSVPPVHHVVP
                     VRVPPGKKATGSILVFPPAPDWDKPQKPAS"
     gene            complement(1131..1859)
                     /locus_tag="VFM00_11635"
     CDS             complement(1131..1859)
                     /locus_tag="VFM00_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HET7498941.1"
                     /translation="MFAGAMDAVGGGGGLVTVPALLAAGLPPHLALGTNKGQSVFGSF
                     AALSRYAHAGLVDRGAALFAFPLAVAGSAAGVALALAIRPGVLRPIVLVLLIAAAVVV
                     LLVRPPEERGDDRVLGRRGDAIWAAAALGIGAYDGFFGPGTGTFLILVLVALLHRPLT
                     RATADAKVVNFGSNLAAVAFFASRGSILWSVALPMAGAQLVGGFVGAHLAVRRGDRLI
                     RAVVVFVVAALVVKIAWDLFTQGT"
     gene            complement(1940..2671)
                     /locus_tag="VFM00_11640"
     CDS             complement(1940..2671)
                     /locus_tag="VFM00_11640"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7498942.1"
                     /translation="MSGVALRETLAFLERHLPPPPARLLQVGAGDGAVALALARRRYA
                     VTALDEAPAPERGDVPLEWIEAEFPHFTPDEPFDAILFTNALHALQPAGRTLDRALAA
                     LLPGGLLLAEELAFDRVNVHTARWFYDLESVLVAAEAIEPPDPAHAAERRPLARWREE
                     HATDPPFASGHDMLAAARERLDLSLVEEAPFLYRGFAARAKDGPRAGPRAGRVLEVVF
                     ELESRLVRERDIAAAGLRFAGKPLL"
     gene            complement(2685..4991)
                     /locus_tag="VFM00_11645"
     CDS             complement(2685..4991)
                     /locus_tag="VFM00_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7498943.1"
                     /translation="MRSPLARYAHWLHTRWPAGTVEKLPEVRPDGTTNVPGVSVTGDL
                     TGVPLLKNALDTGARAVERIALELEAAGGSRGSGSGSGSGNAPTPGSGPDVLDLAIVG
                     AGVSGMAAAVEAARRGLSFRVLESAEPFATVANFPRRKPIFTYPTGVRPAGTLQVTAD
                     VKEALLEELRGQAARAGIEVEAGHAVRVAREPGGVLAVHRVEGAPIRARRVLVAIGKS
                     GDHRRLDVPGEDRDKVANRLHDPKDFAGQNVLVVGGGDSAVETALLLEEAGARVTLAH
                     RGPELARPKPENVERLCRSRVALRLSTRVREIGAADVLLEEGGRTERVPNDAVFTMIG
                     RDAPLGFFRRSGIAIAGEHGASSWIGMGLFLLFCAWLYNWKSGGSMSALFAARSWFPF
                     NLGALLAAAGGAIARDAADPRTLIGTLAISASGPAFWYTLAYSAVVVIFGIRRIRRRR
                     TPYVTAQTATLAAIQVLPLFLLPEIILPLLGRHGLLPRGVLDALFPVVSYGHGREYWR
                     AYGLILAWPLNVYNVFTHDPLPWWLAISFVQTFVLIPLGIWFFGKGVYCGWICSCGAL
                     AETLGDTHRHKMPHGPGWNRLNLAGQGILAIAFLLLAVRIAGWVLPDGNLVDRIFDSA
                     FKDRYKWIVDVFLAGVVGYGLYFWFSGRVWCRFFCPLAALMHLFARVSRFAIVPDKKK
                     CISCNVCTSVCHQGIDVMNFANKGEPMRDPECVRCSACVHACPTGVLTFGQVDRAGRT
                     IALDLLPASPVQMREADARSRALPRTAP"
     gene            5114..5740
                     /locus_tag="VFM00_11650"
     CDS             5114..5740
                     /locus_tag="VFM00_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498944.1"
                     /translation="MTRARRTAPTDSSALPDLPDLEDRAQEHLRFIRSTMERAGSFTA
                     VPGWGGMIMGVTALAAAWIASRAVSPEAWLSTWLVESVAAFLIGCLAIRVKAARSGTP
                     LFDGSARRFAMTLAPPIAAGAIVTLALAKTGTMGVLPGLWLLLYGAAVVTGGASSVRA
                     VPLLGVSLMALGAAALAAPPAWGNAFLAAGFGGLQIGFGLYIARRHGG"
     gene            5733..6119
                     /locus_tag="VFM00_11655"
     CDS             5733..6119
                     /locus_tag="VFM00_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET7498945.1"
                     /translation="MAERSLKRRIDGADVHRDRARGKAEPAAPPESLAALDRLIHERV
                     RLGIVSALAVNESLTFQELRDLLGTTDGNLSVHARRLEEAGYIACKKTFALRMPRSEY
                     RLTPEGRRALDRHLEHLAAVIRWARS"
     gene            6230..6913
                     /locus_tag="VFM00_11660"
     CDS             6230..6913
                     /locus_tag="VFM00_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498946.1"
                     /translation="MVFVPPFAPFVVLAFLALGGVLAVTALALIGAAVFRKTRTIPWI
                     LGVGLVVVVGYAGVLLADSAVSRERILVPGEKKYFCEIDCHLAYSVESVETAPTVGPP
                     LQPLRARGRWHVVRLRTWFDPSTISPSRGDAPLTPNPRVAYVRDRYGKRYEPSWPAEQ
                     ALAAAGRPSTALTTPLRPGQSYETLLAFDLPADAREARLYLGDDDAVSFLLIGHEASP
                     FHRKVWFRI"
     gene            complement(6960..9380)
                     /locus_tag="VFM00_11665"
     CDS             complement(6960..9380)
                     /locus_tag="VFM00_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7498947.1"
                     /translation="MRQLAELWGETDLIAALRRGADLLAAAAGGVPSALFLLTDDGIA
                     AEAWASDESARDLERRARFQRAALEGPHADPRHFGPSGAAVTRRTVPSAGNARATLAW
                     VCGARAGAEDHASGRTGGEAPQAGIAASILDILVARAASLRDADYHRARQEQYERWFK
                     TLDAQLRILDRERQKFAAVANQSDTCAFVTDASHAVTWHNKAMAQLFGNESDQGWTGR
                     PCVDLCGRLGLTEPGTCAGCPVRRAFESNAAVHREVHAPDGRELYLTALPIKGPDGRA
                     FEVLGQIQDLTNLEVVRRAKDRIQTVISGAPIVLFAVDRDGVFTLSEGKGLEAMGLLP
                     GEVVGKSAFDLYRDTPSIEACIHRALAGETVTDSVELGPLAYDAYYVPLRNAEGAILG
                     VIGVATDITERRRAERALQESEEKLRHAQRLEAVGSLAGGVAHDFNNLLTAMFGHLKF
                     LRDRAGSSGEMCLEIDAVEHAANRAATLTRQLLAFSRKQVLQLRAVDLNQIVREMEPM
                     LKRLIGEDVTMVAQLGAERPWIRADAGQVEQVVINLVVNARDAMPSGGRLALRTLDVA
                     SGALPGALPGVSRDGVPEAGRLEASRSGSFVGLVVSDTGCGMDAATLERAFEPFFTTK
                     AVGEGTGLGLSTVYGIVKQSEGEVFVTSAPGEGTTFTLYFPSASEPASEAPRPGAIPA
                     LVPGHETILLVEDEPMVRDLFRDVLEAAGYRVLEAANGIEALQASAAFEGAIDLLVTD
                     VVMPGMAGGELVRRMAEVRPGTKVLYVSGYTDDALVRRGVIEAGVSFLHKPCMPDELS
                     MVVRQILG"
     gene            complement(9427..9909)
                     /locus_tag="VFM00_11670"
     CDS             complement(9427..9909)
                     /locus_tag="VFM00_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7498948.1"
                     /translation="MTVDQLIARLDQLSKEGAPLERLLEEAVRGLHETDPRFHWTGVY
                     ELFEDNMLRLGPFVGAPTDHVFIGVGNGVCGTAVAERRNMNVPDVREITNYLACSTET
                     RSELVVLIRDGDTIYGQIDIDSHRVAAFDESAVGGVQRVADWLAKAYAAKASPRRTPA
                     "
BASE COUNT         1495 a         3490 c         3579 g         1422 t
ORIGIN      
        1 atgtcccgcg aagtgctcga gcaggtggta gtagtaccag cgcacgttca agagacggat
       61 ggagccgtcc ggacgctcgc gtgggatctc gcgatccaga tcttccggca cgcggtcctt
      121 ggccatcgac ttgaggtagc cgcgaccgcg atcgagcaga tcgtcgtaga acgcgaggtc
      181 ctttccgttc aagggcgcat aggccggcgc tccctcgggg agaggcaggc cgtcgtcgtc
      241 cccgccgatg cccaggacgt cgtccacgtc ggcggcgggc tcgccgccga gcgcgaactt
      301 cgtccaccac acctccacga cggccaggtg cgcgaggagc atgccgatgg tgttcattcc
      361 cgccgccggc tgccactgga gatcatccgg ggtgaggccg cgggtggctt ccttcaccag
      421 ccgcagctgg tcgtcgagct gcgcgacgaa cagccccgct tccgcggagc ggaagtccgt
      481 cagcggcgcg agcgtggatc ggacgacggt ctgcatggtg cctccctgag gagaacccgg
      541 cttacgatgc cggcttctgc ggcttgtccc agtccggagc gggcgggaac acgaggatgg
      601 aaccggtcgc cttcttgccc ggcggaacgc gaaccggcac gacgtggtgc accggcggaa
      661 cgctgcgcag ataggccacc accgccttgg cgtcggccgg ggtcagatgg gcgaaatcgg
      721 gccagggcat cggcggcgcg atgggcgatc cgtcgcgctt cacgccgttc tggatcgcgc
      781 ggagaatctc ctcgtcgctc caggcggcga gccccgtcga atccggggtc aggttgcgcg
      841 ggtaggagac gccccacggt ccctgccatc ccagctcgct tcccgagagc tggcggttca
      901 tgtcgggcgc gccgtagagg gtgcccgggg tgtggcagtc gtgacagccg agcaccgtga
      961 ccaggtactg gccgcgctga accggcgtca tcgccgcctc ggcctgcgcg gccgagtcgg
     1021 ccggggcgct tccgctctgc cccgtgttct tggaacagga gacggcggcg acggcgatcg
     1081 tgacgaggag tgctgcgcgg tgaatccgat ccattcctga ttctcctttc tcaggttccc
     1141 tgcgtgaaca agtcccacgc gatcttgacg accagcgctg cgacgacgaa cacgaccacg
     1201 gcgcggatca gccggtcccc ccggcgcacg gccaggtggg ccccgacgaa tccgcccacg
     1261 agctgcgccc cggccatggg gagcgcgacg ctccagagaa tcgagccgcg actcgcgaag
     1321 aacgcgacgg cggcgagatt cgaaccgaaa ttcacgacct tggcgtccgc cgtcgcccgc
     1381 gtgagcggac ggtgcagcag ggccaccagg accaggatca ggaaggtgcc cgttcccggt
     1441 ccgaagaatc cgtcgtaggc cccgatgccg agcgcggccg cggcccagat cgcgtcccct
     1501 cgccgaccga ggacgcgatc gtcgccgcgc tcctccggcg gccgcaccag gaggacgacc
     1561 acggcggccg cgatcaggag cacgagcacg atcggacgca gcaccccggg ccggatcgcc
     1621 agcgccagcg ccacgccggc ggccgagccg gcgaccgcga gcggaaacgc gaacagggcg
     1681 gctccccggt cgaccaggcc cgcgtgcgcg tagcgcgaaa gcgcggcgaa cgagccgaag
     1741 accgactgcc ccttgttcgt cccgagcgcc aggtgaggcg gcaaccccgc cgcgaggagc
     1801 gcgggcacgg tcacgagccc tccgccgccg cccaccgcgt ccatggcgcc cgcgaacatc
     1861 gtggccgcgc agagcgccgc gatggtggtg gccgagagtt ccgccgtcgc ctccttcgga
     1921 tcgggttacg agcgcgggat tatagcaggg gcttccccgc gaaccgaagc cccgccgcgg
     1981 cgatgtcccg ctcgcgcacg aggcggctct ccagctcgaa cacgacctcc agcacgcgcc
     2041 cggcgcgggg ccccgcgcgc gggccgtcct tggcgcgcgc ggcgaagccg cggtagagga
     2101 agggagcctc ttccaccagg gagagatcca ggcgttcacg agccgcggcc agcatgtcgt
     2161 gccccgaggc gaacggcgga tcggtcgcgt gctcctcgcg ccatcgcgcg aggggacggc
     2221 gctcggcggc gtgcgccggg tcgggaggct cgatcgcctc cgcggcgacg agcaccgact
     2281 cgaggtcgta gaaccaccgc gccgtatgca cgttcacgcg atcgaacgcc agctcctcgg
     2341 cgaggaggag cccgccgggc aagagcgcgg cgagcgcgcg atccagcgtc cgtccggccg
     2401 gctggagggc atgcagggcg ttcgtgaaga ggatcgcgtc gaagggctcg tccggagtga
     2461 agtgcgggaa ctcggcctcg atccactcca gcgggacgtc gccgcgctcg ggagcgggcg
     2521 cctcgtcgag ggccgtgacg gcatagcgcc ggcgtgcgag cgcgagggcc accgctccgt
     2581 cgcccgctcc gacctggagc aggcgcgccg gcggtggcgg cagatggcgc tccaggaagg
     2641 cgagcgtctc gcgaagggcg acgccgctca tgacggggat cggctcacgg cgcggttctt
     2701 ggcagggcgc gggaccgggc gtccgcttca cgcatctgga cgggcgaggc gggaagaaga
     2761 tcgagggcaa tcgtccggcc cgcgcggtcc acctgaccga acgtgagcac gcccgtgggg
     2821 caggcatgga cgcacgccga gcatcggacg cattccgggt cgcgcatcgg ctcgcccttg
     2881 ttcgcgaagt tcatcacgtc gatcccctgg tggcagaccg aggtgcagac gttgcaggag
     2941 atgcacttct tcttgtcggg aacgatggcg aaccggctca cgcgggcgaa caggtgcatg
     3001 agcgcggcga gcgggcagaa gaaacggcac cagacccgcc ccgagaacca gaagtagagc
     3061 ccgtagccca ccacgccggc gaggaagacg tccacgatcc acttgtagcg gtccttgaag
     3121 gcggagtcga agatccggtc gacgagattg ccgtcgggaa ggacccagcc ggcgatccgc
     3181 acggcgagca gcaggaacgc gattgcgagg atgccctgcc ccgccaggtt gagccggttc
     3241 cacccggggc cgtgcggcat cttgtggcga tgcgtgtcgc ccagcgtctc cgcgagcgcg
     3301 ccgcaggagc agatccatcc gcagtagacg ccctttccga agaaccagat cccgagcggg
     3361 atcagaacga aggtctgcac gaagctgatc gcgagccacc acgggagcgg gtcgtgggtg
     3421 aacacgttgt agacgttgag cggccacgcc aggatgaggc cgtaggcgcg ccagtactcg
     3481 cggccgtggc cgtagctcac gacgggaaag agcgcgtcca ggacgccgcg cggcaggagg
     3541 ccgtggcggc ccagcaacgg aaggatgatc tcggggagca ggaagagcgg gaggacctgg
     3601 atcgcggcca gcgtggccgt ctgcgccgtg acgtagggcg tgcggcggcg gcggatccgc
     3661 cggatgccga agatcacgac gacggcgctg taggcgagcg tgtaccagaa cgcggggccg
     3721 gaggcgctga tcgcgagcgt tccgatcagg gtgcgcggat cggcggcgtc gcgggcgatc
     3781 gcgccgcccg ccgcggcgag aagcgcgccg agattgaacg gaaaccacga tcgcgcggcg
     3841 aagagggcgg acatgctgcc gcccgatttc cagttgtaga gccacgcgca gaagagaagg
     3901 aagagtccca tcccgatcca ggaggaggcg ccgtgctccc ccgcgatcgc gattcccgac
     3961 cggcgaaaga accccagcgg cgcgtcccgt ccgatcatcg tgaagaccgc gtcgttgggg
     4021 acccgctcgg tgcgtccccc ttcttcgagg agaacgtcgg ccgcgccgat ctcgcggacg
     4081 cgggtcgaca ggcggagcgc cacgcgagac cggcagagcc gctccacgtt ctcgggcttg
     4141 ggacgcgcca gctccgggcc gcggtgcgcc agcgtcacgc gcgcccccgc ctcctccagg
     4201 agcagtgccg tctcgaccgc cgaatcgccc ccgcccacga cgaggacatt ctgtcccgca
     4261 aagtccttgg ggtcgtgaag ccggttcgcg accttgtcgc ggtcctcgcc gggaacgtcc
     4321 agcctgcggt gatccccgct ctttccgatc gcgaccagca cccgccgcgc ccggatcggc
     4381 gctccctcga cgcggtgcac cgcgaggact ccgcccggct cgcgcgcgac gcggacggca
     4441 tgtcccgctt cgacctcgat tccagcccgc gccgcctgtc ctcgcagctc ctccagcagc
     4501 gcttccttga cgtccgcggt gacctggagg gtgcccgcgg ggcgcacgcc cgtcgggtag
     4561 gtgaagatcg gcttccgcct cgggaagttc gcgacggtgg cgaagggttc cgcgctctcg
     4621 aggacccgga acgagagccc gcggcgcgcc gcctcgaccg cggccgccat tcccgagacc
     4681 ccggcgccga cgatcgcgag atcgagtacg tcagggccgg agccaggtgt gggggcgttg
     4741 cccgagcccg agccggagcc ggagccgcgg cttccacccg cggcctcgag ctcgagggcg
     4801 atcctctcga ccgcgcgggc gccggtgtcg agggcgttct tcaagagggg cacccccgtg
     4861 aggtcgccgg tcacggagac gcccggcacg ttggtggtgc cgtccggccg gacttccggg
     4921 agcttctcga ccgtccctgc gggccagcgc gtatgcaacc agtgggcgta acgggccagg
     4981 ggggagcgca ttcggggact atactcgggg tggggcgccc tgtgacgcgg cgggtgatgt
     5041 ggaccacgcc tgccattcac tttgcgtcac aaagtacttt acagatcgtc gccgagagcc
     5101 gatacttccc ctcatgaccc gcgcgcgccg taccgccccg accgactcct ctgctctccc
     5161 cgatctcccc gatctcgaag atcgcgccca ggagcatctg cgcttcataa ggagcacgat
     5221 ggagcgcgcc ggttccttca cggccgtgcc gggatggggc ggcatgatca tgggagtcac
     5281 cgcgctggcg gcagcctgga tcgcttcccg ggccgtgagc cccgaggcat ggctctcgac
     5341 atggctggtc gagagcgtcg ccgcgttcct gatcgggtgc ctggcgatcc gggtcaaggc
     5401 cgcacggtcg gggacgccgc tcttcgacgg ctcggcgcgc cgcttcgcga tgaccctggc
     5461 ccctccgatc gcggccggcg ccatcgtcac gctggccctg gccaagaccg gaacgatggg
     5521 cgtcctcccc gggctctggc tccttctcta tggagcggcg gtggtcaccg gcggagcctc
     5581 ttcggtgcgc gccgtgccgc tccttggcgt ttccctcatg gcgctgggcg ccgcggcgct
     5641 cgcggccccg ccggcctggg ggaacgcgtt cctggccgcc ggcttcggcg ggctccagat
     5701 cggattcgga ctctacatcg ccaggaggca cggtggctga acgttcgctc aaacgacgca
     5761 tcgacggtgc cgacgtccac cgggaccgcg cgcggggcaa ggccgagccg gcggcgccgc
     5821 ccgagagcct ggccgcgctg gatcgcctga tccacgagag ggtgcggctc gggatcgtga
     5881 gcgcgctcgc ggtgaacgaa tcgctcacct tccaggagct gcgcgacctc ctgggcacca
     5941 ccgacggcaa cctgtccgtc catgcgcgac gcttggaaga ggcgggctac atcgcctgca
     6001 agaagacctt cgcgctccgg atgccgcgtt ccgagtaccg gctgacgccg gaagggcgcc
     6061 gggcgctgga tcgccacctg gagcatctcg cggctgtcat ccgctgggcg cgctcgtaga
     6121 gcggcgacgc gccgcgcagc gcgagacctc tcgcgggcgc gtcctggcgg tgttctcccc
     6181 atgaagcttt gtaatgcaaa gtactttgcc cacagaaagg acgcgcgcca tggtctttgt
     6241 cccccccttc gcccccttcg tcgtcctcgc cttcctcgcc ctgggcggcg tcttggcggt
     6301 cacggccctc gcgctcatcg gcgcggccgt gttccgcaag acgcggacga ttccctggat
     6361 tctgggggtt ggcctggtgg tggtcgtggg gtacgcaggg gttctcctcg ccgattccgc
     6421 ggtgagccgc gagcggatcc tcgtcccggg cgagaagaag tacttctgcg agatcgattg
     6481 ccacctggcc tactcggtcg agagtgtcga gacggcgccg acggtcgggc cgccgctcca
     6541 gccgctgcgc gcccgcggcc gctggcacgt ggtccggctc cgcacctggt tcgatccaag
     6601 cacgatctcg ccctcccgcg gcgacgcgcc gctaacgccc aacccgcgcg tggcctacgt
     6661 gcgggaccgt tacggcaagc gatacgagcc gtcctggccc gcggagcagg cactggcggc
     6721 ggcgggacgc ccgagcaccg ccctcacgac gccgctccgc ccgggccagt cctacgagac
     6781 ccttcttgcg ttcgaccttc ccgcggatgc ccgggaggcg cgcctctacc tgggcgacga
     6841 cgacgcggtc tcgttcctcc tgatcgggca cgaagcgagc ccgttccatc gcaaggtgtg
     6901 gttccggatc tgaggccggg cgccggcccc gggaggacgc cgcggatcag ccgaggagat
     6961 cagccgagga tctggcgcac gaccatcgag agctcgtcgg gcatgcaggg cttatgcagg
     7021 aaggaaacgc cggcctcgat gacgccgcgg cggaccaggg cgtcgtcggt gtagccggag
     7081 acgtagagca ccttcgtccc ggggcgaacc tcggccatgc ggcggaccag ctcgcccccg
     7141 gccatgccgg gcatgacgac atcggtcacg agaaggtcga tcgcgccctc gaaggccgcg
     7201 gaggcctgca gggcctcgat cccgttcgcg gcttcgagca cgcgatagcc ggcggcttcg
     7261 aggacgtccc ggaagaggtc gcgcaccatc ggctcgtcct cgacgagaag gatggtctcg
     7321 tgtccgggca cgagcgccgg aatcgcgccc ggacgcggcg cctcggaagc gggctcggag
     7381 gcgctcggga agtagagggt gaacgtggtt ccctccccgg gcgcgctggt gacgaagacc
     7441 tctccctcgc tctgtttcac gatgccatat acggtcgaga ggccgagacc cgtgccttcc
     7501 ccgacggcct tggtcgtgaa gaacggctcg aaggcgcgct ccagggtcgc ggcgtccatg
     7561 ccgcatccgg tatcgctgac gaccaggccg acgaacgagc cggaacgcga cgcttcgagg
     7621 cgcccggcct ccggcacgcc gtcccgggag acaccgggca atgcgcccgg aagggcgcct
     7681 gacgcgacat cgagcgtgcg gagcgccagg cggccgcccg acggcatcgc gtcgcgcgcg
     7741 ttcacgacga ggttgatcac gacctgctcg acctggccgg cgtcggcccg gatccagggc
     7801 cgctcggcgc ccagctgggc gaccatcgtg acgtcctcgc cgatcagccg cttcagcatc
     7861 ggctccatct cgcgaacgat ctggttcaga tccaccgcgc ggagctggag cacctgcttc
     7921 ctgctgaagg cgaggagctg ccgggtcagc gtggccgcgc gattcgcggc gtgctcgacc
     7981 gcgtcgatct ccaggcacat ctcgccggag gagccggcac ggtcgcgcag gaatttcaga
     8041 tgtccgaaca tcgcggtgag gagattgttg aagtcgtggg ccacgccgcc ggccaggctt
     8101 cccaccgctt cgagtcgctg ggcgtgccgg agcttctcct cgctctcctg gagcgcgcgc
     8161 tccgccctgc gccgctcggt gatgtcggtc gcgaccccga tcacccccag gatcgcaccc
     8221 tcggcattcc ggagcgggac gtagtaggcg tcgtaagcga ggggcccgag ctcgacggaa
     8281 tcggtcacgg tctcgcccgc gagcgcccgg tggatgcacg cttcgatgct cggtgtatcg
     8341 cgatagagat cgaacgccga cttcccgacc acttctccgg gcaggagccc catcgcctcg
     8401 agaccctttc cttccgacag ggtgaaaacc ccgtcgcgat ccacggcgaa gaggacgatc
     8461 ggcgcgccgg agatgacggt ctggatgcgg tccttcgcgc gccgaacgac ctcgaggttc
     8521 gtgaggtcct ggatctggcc caggacctcg aaggcccggc cgtccggccc cttgatcgga
     8581 agcgccgtca ggtacagctc gcgtccatcc ggagcgtgca cctcgcggtg gacggccgcg
     8641 ttcgattcga acgcccgtcg cacggggcag cccgcgcacg ttcccggctc ggtgagaccg
     8701 agcctgccgc acaggtcgac gcagggacgt ccggtccagc cctggtcgga ctcgttgccg
     8761 aagagctgag ccatggcctt gttgtgccag gtgaccgcgt gtgacgcgtc ggtgacgaac
     8821 gcgcaggtgt cgctctggtt cgcgacggca gcgaacttct ggcgctcccg gtcgaggatg
     8881 cggagctgcg cgtcgagcgt cttgaaccag cgctcgtact gctcctgtcg cgcgcggtgg
     8941 tagtcggcgt cgcggaggga agcggccctg gcgacgagga tgtccaggat cgacgccgcg
     9001 attccggcct gcggagcctc gccgcccgtt cgaccggacg cgtggtcctc cgcaccggcg
     9061 cgggccccgc acacccaggc gagcgtcgcg cgcgcgttcc cggccgaggg aaccgtccgg
     9121 cgcgtcacgg cggccccgct cgggccgaaa tgacggggat cggcgtgcgg accctcgagc
     9181 gcggcgcgct ggaagcgcgc ccgccgctcc agatcgcgcg cgctctcgtc ggacgcccac
     9241 gcctcggccg cgattccgtc gtcggtgagg aggaagaggg ccgacggaac gccgcccgcg
     9301 gcggcggcga ggagatcggc gccgcggcga agcgccgcga tcaggtccgt ctcgccccac
     9361 agttccgcaa gctgccgcac gatccggctc gcggcgtcgg gcggttgcgg gggcgagctc
     9421 atccggtcag gccggcgtgc gccggggcga ggccttcgcg gcgtacgcct tggcgagcca
     9481 gtcggcgacc cgctgcacgc cgccgacggc gctctcgtcg aaggccgcga cgcggtggct
     9541 gtcgatgtcg atctggccgt agatcgtgtc gccgtcccgg atgagaacga ccagctcgga
     9601 gcgggtctcg gtgctgcagg cgaggtagtt cgtgatctcg cgcacgtcgg ggacgttcat
     9661 gttgcgccgc tcggcgaccg ccgtgccgca gacgccgttc cccactccga tgaagacgtg
     9721 gtcggtcggg gcgccgacga acggccccag ccgcagcatg ttgtcctcga acagctcgta
     9781 gacgcccgtc cagtggaagc gcgggtcggt ctcgtggagc ccgcgcaccg cctcctccag
     9841 caggcgctcg agcggagcgc cttccttgga gagctggtcc aagcgcgcga tcagttggtc
     9901 gacggtcatg gtttggtcct cggatgcagg ggagtttgcc gcgctccgcc gaggcggagc
     9961 gcggcgaaga tggatcggca cggagc
//
LOCUS       DASSLZ010000310         6969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_111638, whole genome shotgun sequence.
ACCESSION   DASSLZ010000310 DASSLZ010000000
VERSION     DASSLZ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111638"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VFM00_11675"
     CDS             <1..1063
                     /locus_tag="VFM00_11675"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HET7498949.1"
                     /translation="DVINLSSNNYLGLTTHPKLREAAIGAVKELGAGSGSVRTIAGTM
                     DIHMELERKIAAFKKTEAAVVFQSGFTANAGTVSSILGKEDLILSDELNHASIIDGAR
                     LSRATIKVFPHRDTDALRKLLEETTEWKRRLVITDGVFSMDGDIAPLPAIAALAKEHG
                     AITMVDDAHSSGVLGKNGRGTIDHYGIHGQVDIQVGTLSKAIGVLGGYVCGSKSLIEY
                     LYHRARPFLFSTSHPPAVAAACIAAFEVLEQEPERIERLWSNTRRFKAGLERLGFDTG
                     ISETPITPIIVGEADRAMKLSDLAFERGVFAQGIGYPTVAKGRARLRTIVTATHTEEE
                     LDRALEVLGDAARSLGILS"
     gene            1198..2073
                     /gene="waaF"
                     /locus_tag="VFM00_11680"
     CDS             1198..2073
                     /gene="waaF"
                     /locus_tag="VFM00_11680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HET7498950.1"
                     /translation="MARAPGARYLTAARTRRLALARELRALEPGAALLLPDSFSSALV
                     AALARIPRRIGYDAEWRGFLLTVRVPRAGRARTAPRTAEYRVLAEAAGLSVPPGEPSL
                     AAASGERALGLARLEAAGLGTGPVLVLAPGAAYGPAKQWGPERFAAVGAHVARTRGAA
                     LAIVGAGGDAAPAGETARLAAAAGARVANLAGATSIPELVGILDAALAVVSNDSGVMH
                     LAAALGRPTVGVFGSTSPVWTASAAPWVTSLYAEYPCSPCFRRTCPIGYGCLRSIEAE
                     QGTNALERLLGREKE"
     gene            complement(2078..3169)
                     /locus_tag="VFM00_11685"
     CDS             complement(2078..3169)
                     /locus_tag="VFM00_11685"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET7498951.1"
                     /translation="MALQGNLDDFSLPEILQLIAVQQKSGVLKLTAGDEVGVIFFEGG
                     KVVSTRDRRRNAKDPLKPFLVKTGRLTDPQLRQVETIENESRRELTDILLSGNYLTSE
                     QLSHAVEEQIQDTLHQLLTWKTGTYHFSGDSRTVPKFAVNVRLNTEGLLMESMRRIDE
                     MARYKEELSSTAMVLRPKALAVPPKEMTEAERRVLPLIDGLRPIRDIMAQSKLVDFEV
                     YEALHHLLEQGVVEISLSAAPPKVTPMPSATAPRAAQPRGALALAIGTLVLALSLGLG
                     VYGSPALLAPVQRSATPAPWAPPAIAGVDDAARVTLALETYRTMRGKYPGELATLARA
                     GLVSASTVQAVSRRYLYKSDGKTYSRSPR"
     gene            complement(3173..5065)
                     /locus_tag="VFM00_11690"
     CDS             complement(3173..5065)
                     /locus_tag="VFM00_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498952.1"
                     /translation="MAPAPRQLAVVAILACLACAFPLAPRAIAAPLDDAAIAAALETK
                     YDRGVARDLPMAQACYREALERSAAGARPEAIRLLEAASLFDPDYPDPHFTIARLLVT
                     SEPGRAVGEAAEAFRILGRSYPWQRHLLANAVTALVVVWMLSLLLAVVGIVFRHLPHL
                     VHVVAESLGARGSRLGGFGAGLIAIAPALWGLGAAATATVFAGLVSFRLGKREALLVL
                     LFVASALALAGWSGSVAPWAGAPRLDEPSLLVDRALSSGYDPDVARAIQAWEARDANE
                     ALFPFALGTLRRRAGDLDGAAEQLTLAAVLRPNSPWILTGLGNVYFAREDYARARQLY
                     ESGAAADPGAVEPHYNLAQVYTKQLLFAEASREQSRASALAFDRVRDFTRMGAPQLNR
                     TVMDARPPVQTLWDLAARTGHLRGPATIEQSRYLKFALSLTPPAPFALVFLPALFLIF
                     AGIGQVLGRSLATLHCSNCQKIVCRRCVHRMQQRAFCNDCFRTVKDLKSMEFTRLLLT
                     RRDRSAARRRTIGQAVLTFLLPGGGQMLRGATLTGFFALLVMTAAGLLVIQNGALVPS
                     LDVSPIPGSDWAKRAPLLLLFLLTYAITVSRYFATTTATVERLTPGEPKTARGGSGRR
                     AGAGRS"
     gene            5180..6478
                     /gene="asnS"
                     /locus_tag="VFM00_11695"
     CDS             5180..6478
                     /gene="asnS"
                     /locus_tag="VFM00_11695"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET7498953.1"
                     /translation="MSREGTRIEALPSKIGQQVTLEGWLMNRRSSGKIEFLQVRDGSG
                     VVQCVAVRSDLPEETFARCASATQESAVRVKGVVREDKRAPSGVELTLTDLEIVAPSK
                     DYPITPKEHGTPFLLDHRHLWIRSQRQHAILRIRHTVVDACRDFLNRDGFILADAPIF
                     TPNACEGTTTLFETDYFGEKAYLTQSGQLYNEATAMALGKTYCFGPTFRAEKSKTRRH
                     LIEFWMVEPEVAYATLDDVMDLIERFVTSVVERVLTDRKTELRVLERDTAPLERVKPP
                     FPRIAYDEAAQILVKKGLPFETGNDFGGTDETVLTEGFDRPFFVHRFPAAVKAFYMKR
                     DPRDERLSLSCDLLAPEGYGEIVGGGEREDDIEKLAGRVAEHGLPEEAFRWYLDLRRY
                     GSVPHAGFGMGIERALTWICGIDHVRETIPFPRMLHRLKP"
     gene            6571..>6969
                     /locus_tag="VFM00_11700"
     CDS             6571..>6969
                     /locus_tag="VFM00_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498954.1"
                     /translation="MRRLLALLLVALASVPSLAARPPEVRAADGRAIYRIVPPKGGAA
                     RLLGLGFDPAGYGPGSSLDFILTPEEAARARSLGFTPVALSTRGGPLAAPAAPGLGAY
                     HTYGEALSEMASYASAHPSIARLDTLGLSLE"
BASE COUNT         1027 a         2420 c         2527 g          995 t
ORIGIN      
        1 cgacgtcatc aacctctcct cgaacaacta tctcggattg acgacccacc ccaagctccg
       61 cgaggccgcg atcggcgccg tgaaggagct gggggcggga agcggctcgg tgcggaccat
      121 cgccggcacc atggacatcc acatggagct ggagcggaag atcgccgcct tcaagaagac
      181 cgaggcggcc gtcgtcttcc agagcggctt caccgcgaac gcggggacgg tgtcctcgat
      241 cctgggcaaa gaggacctga tcctctccga cgagctgaac cacgcgagca tcatcgacgg
      301 ggcgcgcctc tcgcgcgcca cgatcaaggt cttcccgcat cgcgacacgg acgcgctccg
      361 gaagctgctc gaggagacga cggagtggaa gcggcgcttg gtgatcacgg acggcgtctt
      421 cagcatggac ggcgacatcg ctcccctgcc ggcgatcgcg gcgctcgcca aggagcacgg
      481 cgccatcacg atggtggacg acgcccactc gagcggcgtg ctcgggaaga acggccgggg
      541 caccatcgac cactacggca tccacggcca ggtggacatc caggtcggga ccctctccaa
      601 ggcgatcggc gttctcggcg gctacgtctg cggctcgaag agcctgatcg aatacctcta
      661 tcaccgcgcc cgccccttcc tgttcagcac gtcgcacccg cccgccgttg cggcggcgtg
      721 catcgccgcg ttcgaggtgc tggagcagga gccggagcgg atcgagcgtc tctggtccaa
      781 tacgaggcgc ttcaaggcgg ggctcgagcg gctggggttc gacaccggga tcagcgagac
      841 gccgatcacg ccgatcatcg tcggcgaggc ggatcgcgcg atgaagctct cggacctggc
      901 gttcgagcgc ggggtgttcg cgcagggcat cggctatccg acggtggcga agggaagggc
      961 gcggctccgg acgatcgtga ccgcgaccca caccgaggag gagctggatc gggcgctcga
     1021 ggtcctgggc gacgccgcgc ggagcctcgg gatcctctcc tgaaggtcgg ggcaggcccg
     1081 ctctacgtcc gcctgccgaa ctggctcggc gacctgatcc tggcctggcc ggtcgtcgcg
     1141 gccgccgcgg agcggggcgc gatcttcgcg ggccgccccg cgttcgagcc tctcgtcctg
     1201 gcgcgggctc ccggcgcgcg ctatctcacg gcggcgcgca cgcgccggct cgcgctcgcg
     1261 cgcgagctgc gcgcgctcga gccgggagcg gccctcctcc tgcccgattc cttctcctcg
     1321 gccctcgtcg ccgcgctcgc gcgcattccg cgccggatcg gctatgacgc cgaatggcgc
     1381 gggttcctgc tcacggtgcg cgtcccgcgt gcaggaaggg cccgcaccgc gccgcgcacg
     1441 gccgagtatc gcgtgctggc cgaggccgcg ggactgtcgg ttccgcccgg ggagccttcg
     1501 ctcgcggcgg cttccggcga gcgggcgctg ggcctggccc gcctcgaggc cgccgggctt
     1561 gggaccggtc cggtgctcgt gctggcgccg ggggccgcgt acggtcccgc gaagcagtgg
     1621 gggccggagc gcttcgccgc ggtcggcgcg catgtcgcgc ggacgagggg agcggcgctc
     1681 gcgatcgtgg gcgcgggagg cgacgccgcg cccgcggggg aaaccgcgcg tctcgccgcc
     1741 gccgcggggg cgcgcgtcgc gaacctcgcc ggcgcgacct cgatccccga gctggtcggg
     1801 atcctggacg ccgcgctcgc ggtggtctcg aacgattccg gggtcatgca cctcgcggcc
     1861 gcgctcggcc gtcccacggt cggggtcttc ggctcgacca gccccgtctg gacggcgtcg
     1921 gcggcgccgt gggtgaccag cctgtacgcc gaatacccct gctccccctg cttccggcgg
     1981 acgtgtccga tcggatacgg ctgtctccgc tcgatcgaag cggagcaggg aacgaacgcg
     2041 ctggagcggc tgctgggaag ggagaaggag tagcgggcta ccggggcgaa cgcgagtagg
     2101 tcttaccgtc ggacttgtag aggtaccgtc tcgacaccgc ctgcaccgtg ctcgccgaga
     2161 ccagcccggc gcgcgccagg gtcgccagct ctccgggata ctttccgcgc atcgtacggt
     2221 aggtctcgag ggccagcgtg acgcgagccg cgtcatccac gcccgcgatc gccggcggcg
     2281 cccacggcgc gggcgtggcg gagcgctgga ccggcgcgag cagggcgggc gatccgtaga
     2341 ctccgagtcc cagggagagc gcgagcacta gggtcccgat cgcgagcgcc agggctccgc
     2401 gcggctgggc ggcccgcggc gcggtcgccg agggcatcgg cgtgaccttg ggcggcgcgg
     2461 cgctcagcga gatctccacc accccctgct cgagcaagtg atggagcgcc tcgtacacct
     2521 cgaagtccac cagcttcgat tgcgccatga tgtcgcggat cggacggagc ccgtcgatca
     2581 gcggcagcac ccgccgctcg gcctcggtca tctccttcgg cggcacggcc agcgctttgg
     2641 gacggagcac catggccgtg gaggagagct cctccttgta gcgcgccatc tcgtcgatgc
     2701 ggcgcatcga ctccatcagg agcccttcgg tgttcagccg cacgttcacc gcgaacttgg
     2761 gaaccgtgcg cgagtcgccc gagaagtggt aggtcccggt cttccaggtg aggagctgat
     2821 gcagggtgtc ctggatctgc tcttccaccg cgtgcgacag ctgctcgctg gtgaggtagt
     2881 tgccgctcag caggatgtcg gtcagctcgc ggcggctctc gttctcgatc gtctcgacct
     2941 gccgcagctg cggatcggtc aggcggcccg tcttgaccag gaagggtttc agcggatcct
     3001 tcgcgttccg ccggcggtcc cgcgtcgaga cgaccttgcc tccctcgaag aagatgacgc
     3061 ccacctcgtc cccggccgtc agcttgagga cgcccgactt ctgctgcacc gcgatgagct
     3121 ggaggatctc gggaaggctg aaatcgtcga gattgccctg gagcgccatc ggtcagctcc
     3181 gtcccgcgcc ggcccgccgt cccgacccgc cgcgcgccgt ctttggctcg cccggcgtca
     3241 ggcgctccac cgtcgcggtg gtggtggcga agtatcggga gaccgtgatc gcgtaggtga
     3301 gaaggaagag gagaaggagc ggcgcccgct tcgcccagtc gctgcccggg atcggcgaga
     3361 cgtcgagcga aggaaccagg gctccgttct ggatgacgag cagtcccgcc gcggtcatca
     3421 cgaggagcgc gaagaacccc gtgagggtcg ctccgcgcag catctggccg ccgcccggga
     3481 gcaggaaggt gagcaccgcc tggccgatcg tgcgccggcg cgcggcggag cggtcgcgcc
     3541 gcgtgagcag gagccgcgtg aactccatcg acttcagatc cttcacggtg cggaagcagt
     3601 cgttgcagaa ggcgcgctgc tgcatccggt ggacgcagcg gcggcagacg atcttctggc
     3661 agttggagca gtggagggtc gccaggctgc gcccgagcac ctgtccgatc ccggcgaaga
     3721 tgaggaagag cgccggcagg aagaccagcg cgaagggcgc cgggggcgtg agcgagaggg
     3781 cgaacttgag gtaccggctc tgctcgatcg tcgccggacc ccggagatgc ccggtgcgcg
     3841 cggccaggtc ccagagcgtc tgaaccggcg gccgggcgtc catgaccgtg cgattcagct
     3901 ggggcgctcc catcctcgtg aagtcccgta cccggtcgaa cgccagcgcc gaggcccgcg
     3961 actgctcgcg gctcgcttcg gcgaagagga gctgcttcgt atagacctgc gccaggttgt
     4021 agtgcggctc gacggcgccg ggatcggcgg cggcgcccga ctcgtagagc tggcgcgccc
     4081 gcgcgtagtc ctcgcgggcg aaatagacgt tgcccaagcc ggtcaggatc caggggctgt
     4141 tcggccggag caccgcggcc agcgtcagct gctcggccgc cccgtccagg tcgcccgcgc
     4201 ggcgccggag cgttccgagc gcgaagggga agagcgcctc gttcgcgtcc cgcgcttccc
     4261 acgcctggat cgcgcgcgcc acgtcggggt cgtatcccga ggagagcgcc cggtccacga
     4321 gcaggctggg ctcgtccagc cgcggcgcgc cggcccacgg ggcgacggag ccgctccagc
     4381 cggccagcgc cagcgccgag gcgacgaaga ggagcacgag cagcgcttcg cgcttgccga
     4441 gccggaagga gacgagcccc gcgaagacgg tcgccgtggc cgccgcgccg agtccccaga
     4501 gcgcgggggc gatcgcgatc aggcccgcgc cgaagcctcc caggcggctg ccgcgcgcgc
     4561 cgagcgattc cgcgacgacg tgcacgaggt ggggaagatg gcggaagacg atgccgacca
     4621 cggcgaggag gaggctcagc atccagacca cgaccagcgc ggtgaccgcg ttcgccagga
     4681 gatgacgctg ccaggggtag ctgcgcccca ggatccggaa cgcctcggcc gcttcgccga
     4741 ccgcgcgtcc cggctccgac gtcacgagga gccgcgcgat ggtgaagtgc gggtcaggat
     4801 agtcgggatc gaacagggag gcggcttcga ggaggcggat cgcctcgggc cgggcgccgg
     4861 cggcgcttcg ctcgagcgct tcgcgatagc aggcctgtgc catcggcagg tcccgcgcga
     4921 cgccccggtc gtacttcgtc tcgagggcgg ccgcgatggc ggcgtcgtcg agcggcgcgg
     4981 cgatggcgcg cggcgcgagc gggaacgcgc acgcgaggca cgcgaggatc gcgacgaccg
     5041 cgagttggcg gggggctggc gccacgggcc gcacgttagg ctccggcccg gcgacggtca
     5101 agggaaatgt cccccgcgca tgctatcctg accgctcgtt cacccccgaa ccaccattcg
     5161 cgcattccgg aggagtcgca tgagtcgcga ggggacgcgc atcgaggcgc tgccgtcgaa
     5221 gatcggccag caggtcacgc tggaaggctg gctgatgaac cgccgctcca gcggcaagat
     5281 cgagttcctg caagtccggg acggaagcgg cgtcgtccag tgcgtggccg tccgctccga
     5341 cctgcccgaa gagaccttcg cgcgctgtgc ttcggccacc caggagagcg cggtccgcgt
     5401 gaagggggtc gtccgcgagg acaagcgcgc gccgagcggc gtggagctca cgctcaccga
     5461 tctcgagatc gtggcgccga gcaaggacta tccgatcacg ccgaaggagc acggaacgcc
     5521 cttcctcctc gaccatcgcc acctctggat ccgctcgcag cgccagcacg ccattctccg
     5581 gatccgccac accgtggtgg acgcctgtcg cgacttcctg aaccgggacg gattcattct
     5641 cgccgacgcg ccgatcttca cccccaacgc ctgcgaggga acgacgaccc tgttcgagac
     5701 cgactacttc ggcgagaagg cgtacctcac gcagagcgga cagctctaca acgaggccac
     5761 cgccatggcg ctggggaaga cctactgctt cgggcccacg ttccgggccg aaaagtccaa
     5821 gacccgccgc catctgatcg agttctggat ggtggaaccg gaggtggcct acgccaccct
     5881 ggacgacgtc atggatctga tcgagcgctt cgtgacgtcg gtcgtggagc gggtgctgac
     5941 cgatcggaag acggagctcc gcgttctgga gcgcgatacc gcgcccctcg agcgggtgaa
     6001 gccccctttt ccgcggatcg cctacgacga ggcggcccag attctggtga agaaggggct
     6061 gccgttcgaa acggggaacg acttcggcgg caccgacgag acggtgctca ccgaggggtt
     6121 cgaccgcccc ttcttcgtgc atcggttccc ggcggccgtg aaagcgttct acatgaagcg
     6181 cgatccccgg gacgaacgcc tgtcgctttc ctgcgacctg ctcgcgccgg aggggtacgg
     6241 ggagatcgtg ggcggcgggg agcgcgagga cgacatcgag aagctcgccg gccgggtcgc
     6301 cgagcacggg cttccggagg aggccttccg ctggtacctg gacctgcggc gctacggctc
     6361 ggtcccgcac gcgggattcg ggatggggat cgagagagcc ctgacatgga tctgcggcat
     6421 cgaccacgtc cgggagacga ttcccttccc gaggatgctc catcggctga agccctgagc
     6481 cggcgggcgg gtgtcgggaa cggggcgccg gaatgctata attaggaagg tcgctcccgt
     6541 cccatcccgc tctgcaccgt cggagacctc ttgcgccggc tcctcgccct gctcctggtc
     6601 gcgctcgcgt cagtcccttc gctggccgcg cgccccccgg aggtccgggc cgccgacggc
     6661 cgcgcgatct accggatcgt cccgccgaag ggcggcgcgg cccgattgct ggggctgggc
     6721 ttcgatccgg ccggctacgg ccccggctcc tccctcgatt tcatcctgac ccccgaagaa
     6781 gcggcgcggg cgcgctcgct gggtttcacg ccggtcgcgc tgtccacgcg cgggggaccg
     6841 ctggccgcgc cggcggcgcc cggactgggc gcctaccaca cgtacggcga ggcgttgtcc
     6901 gagatggcgt cctacgcctc ggcccacccc tcgatcgcgc ggctcgacac gctgggcctg
     6961 agcctcgag
//
LOCUS       DASSLZ010000311         8282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1372585, whole genome shotgun sequence.
ACCESSION   DASSLZ010000311 DASSLZ010000000
VERSION     DASSLZ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372585"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..489)
                     /locus_tag="VFM00_11705"
     CDS             complement(79..489)
                     /locus_tag="VFM00_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET7498955.1"
                     /translation="MSAGRRLRVETGERTQFVPLAARIREEAGSLLEGQNGLLIVSVP
                     HTTAGICVNEGYDPDVMDDVGRVLERLVPWKADYRHAEGNAAAHVKTILTGSSVMIPL
                     RAGEIELGRWQEIFLCEFDGPRTRTVLLTVVPAP"
     gene            complement(495..767)
                     /locus_tag="VFM00_11710"
     CDS             complement(495..767)
                     /locus_tag="VFM00_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498956.1"
                     /translation="MFRLILRLWFTLLLFFLGRLFGGSRRGSFGAPGNDRGPGPGNVG
                     GSRPPRARRGHAPGSIPPIDRSDVVDVPFTEIPPPTDPAHGAAGGP"
     gene            complement(834..1406)
                     /locus_tag="VFM00_11715"
     CDS             complement(834..1406)
                     /locus_tag="VFM00_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498957.1"
                     /translation="MSTGIPQADISARGPLSRADFLALLYWFSDTRAVPEVRGICGLS
                     RLTRLALILGEDTGLSREISPFFTFHTTPNGGVASLDVWTEFLALRDYQVLTPLPADE
                     PMPPEEVKERRYLLENHIPAQERRHYPLPTFFERDVLTNKGTFFAAKREDQLVERRIR
                     IFQTVPDLNRLPLAELTERALPLVRVPAGR"
     gene            complement(1420..2304)
                     /locus_tag="VFM00_11720"
     CDS             complement(1420..2304)
                     /locus_tag="VFM00_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET7498958.1"
                     /translation="MRSRMKFAAAGLGACALAALAIASPAGAAHLRNVSEKVFPFAAG
                     GDIVIESTNGRIVVEAWDRSDVRVQITREVRASTDEMAKALLRELKADVAVHPGEIRI
                     ESVFPRKQKVVGFWDLIGHGVQSMNIHYYLQVPRQASLDLNTSNGAIRVRAVQGRVAV
                     ATNNGDVDVAGVRGTLVASTRNGEVRLARIEGATNASTTNGSIAAEMAALPDRGRMEM
                     TTTNGNIELALPRDLHAELEAETTNGRVNINYKLTTQGTINSKRIRGTIGGGGVRIEL
                     QTTNGNIDVGPPRARASS"
     gene            complement(2414..3496)
                     /gene="ychF"
                     /locus_tag="VFM00_11725"
     CDS             complement(2414..3496)
                     /gene="ychF"
                     /locus_tag="VFM00_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET7498959.1"
                     /translation="MKLGILGPPQSGKTTLFAALTRGQAQAGSARGETVHLGSVRVPD
                     ARLEHLREMYQPKKFTPAKVDYVDAPPLETGRAERGALAALAALRDVDAFVLVLRAFD
                     DPAVPHFQGSVDPARDAGWLLSELILEDLALVERRLERIEKAVKVGKKPEDPNEYDAL
                     KAVRAALESERPARMAGLSKAGERALRGFQLLSMRPWIVVVNADDARLKEGEASLVAP
                     VASRFGAEAPPVIALSAKTEAELVQLSDEDASEFMAVLGIQEPGLTRMIRLSYATLGL
                     ISFFTVGPDEVRAWTVRDGSLAPEAAGAIHSDLERGFIRAEVIAYDDLAKLGGMPKAR
                     EQGLLRTEGRDYRVRDGDVVNIRFSI"
     gene            complement(3539..4216)
                     /locus_tag="VFM00_11730"
     CDS             complement(3539..4216)
                     /locus_tag="VFM00_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7498960.1"
                     /translation="MEESTYTDPRFIQYSKRFTMVKLNAEKDTVTAAHYRVKSYPTIM
                     VLKKDGTELDRVVGYYRARPFMDQVEDYLAGRNTLASYAAMEPDSGKDPGYVGRLAER
                     YLYHGLYDEARMRYKKLVAMDPSNRSGLVDDALYSLARMSRKDKDYGQDARYAQQIID
                     RYPDSDMYKSAILEKAGALRRDMKLAQARTIYLDYAKRFPTDEDAAWAREQADTLSVK
                     IQRGEGA"
     gene            complement(4232..4426)
                     /locus_tag="VFM00_11735"
     CDS             complement(4232..4426)
                     /locus_tag="VFM00_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498961.1"
                     /translation="MKTSALKGWGMAGAFALTLAIGCAQQKHEAAAPQSIAWFTSYDA
                     ALADAGKTGRPILLDFYTDW"
     gene            4540..4923
                     /locus_tag="VFM00_11740"
     CDS             4540..4923
                     /locus_tag="VFM00_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498962.1"
                     /translation="MSACHFCGTSLDPKMRIVKDTVCPSCGRDLHACVQCRHYDRNAH
                     NQCREPQAEWVTDRERRNFCDYYALNPVGGKGAGVPDREKAARSKLDSFFAPGPAKPG
                     PAEARSKLDSLFKPAPGKDPEPDDE"
     gene            4920..5654
                     /locus_tag="VFM00_11745"
     CDS             4920..5654
                     /locus_tag="VFM00_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET7498963.1"
                     /translation="MKKIDVLVFGPHPDDAEIGAGGLLLKMKALGHTTGIIDMTRGEM
                     GWGTPEQRGAECDEACQILKLDVRENLDMGDHRIEDTFENRCTVAAVIRKHRPDLIFA
                     PHYAIPPGRGLGHNDHFKTGILIAQGHNLAHLRKAPLSGEPHQAKAIFYYFLPPGVRP
                     TIVVDVTPYFEDWMRALSCHKTQFSNPDKPKPKGEQFSVVERVETIAKSNAWLIGGKY
                     AQTFYSDAPLKIGDPMHLVQEIEPRP"
     gene            complement(5667..7421)
                     /locus_tag="VFM00_11750"
     CDS             complement(5667..7421)
                     /locus_tag="VFM00_11750"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HET7498964.1"
                     /translation="MPLLTFAVLLIALAAAPSTAAPSAAAPSATPPSAPAQPAPAHTA
                     AGVIAWRPWDRAAFEEARAEKKLVLLHIAASWSRSGRQMDFETWTDTSVVRVVDQGFI
                     PVRVDADRRPDIADRYLSGGWPTTAVLSHDGQVLMAQSVVAPQPLRQMLERVGDLYRQ
                     NRSEIEFRAGESARRIEHTWKSEPLVPPDMTLEDWVDRNLAALREAEDHVHGGIEGSP
                     KQPQFEAVHFLLSASAARGDRALRSLGLRVLDAALHLEDTTWGGFFRIAGDEDWKRPR
                     TEKLLDVNARATAALVGAVEQGGGTRYRDAARRTEAYAATWLWAPRPGGWYGSQAADV
                     VLHQGHRPSQDRDAGGPGRDGGGPGHDSGEPDRGERMLGGDVYYGLSDALRRKQGLPA
                     IDSSLYAEQNARMAAAVLHGAAAGIWKGAPVARAVKALDRLWTAQRAPDGSLYHEWRE
                     GRVAVPGRLMDQAAAGLAYLEAYAATKEPRHLARAESIAVWIRTHLEDPVAGGFRYAP
                     RDSQAIGRLAAGEKPEQGNLDAGWLFFRLWQLKHRPEDRRSAERAMEQLRSGDVVVLD
                     PAQAELGLALEKGTNAKP"
     gene            complement(7409..7975)
                     /locus_tag="VFM00_11755"
     CDS             complement(7409..7975)
                     /locus_tag="VFM00_11755"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET7498965.1"
                     /translation="MSGRDDEHSAKPHATGHPDADGEQRPGRGPMRPPRPALNLPLLA
                     NGCFQLPEGNPCEGCDHCCRYVTIPIDRPRSRRDFDNIRWYLLHRNVSVLCDWEGSWM
                     VQFDTPCDWLKDGRCSHYVHRPEVCRVYDPADCERYCPDPAEKILLRDEKDLERFLNE
                     REARSKASRQAKAAAKTAAKTAKAASRF"
BASE COUNT         1248 a         2916 c         2768 g         1350 t
ORIGIN      
        1 gtccttcgac gcctcgcccc tcggccccgc gcccttcgcc cgcggaggag tccccgaaga
       61 gatccaggga ttcgtcgctc acggggcggg caccaccgtg aggagcacgg tccgcgtgcg
      121 cgggccgtcg aactcgcaga ggaagatctc ctgccagcgc cccaactcga tctcccccgc
      181 gcggagcgga atcatcaccg aggatcccgt caggatcgtc ttcacgtgcg cggccgcgtt
      241 gccttcggcg tggcgatagt cggccttcca gggcacgagc cgctccagga cgcgtccgac
      301 gtcgtccatc acgtcggggt catatccctc gttcacgcag atgccagccg tcgtgtgcgg
      361 gacgctcacg atcaggaggc cgttctgtcc ctccagcaac gatcccgctt cctcgcggat
      421 ccgcgcggcg agaggcacga actgcgtccg ctcgcccgtc tcgacacgaa gcctccttcc
      481 agcggacatg cgctctaggg ccctccggcg gcgccgtggg cggggtccgt cggcggcggg
      541 atctcggtga agggaacgtc cacgacgtcg ctgcggtcga tcggggggat gctgcccggg
      601 gcgtggccac ggcgcgcgcg gggcgggcgc gatccgccca cgttgcccgg ccccggtccg
      661 cggtcgtttc ctggcgcgcc aaagctccca cggcgtgagc ccccgaagag acggcccagg
      721 aagaagagca ggagcgtgaa ccagaggcgg aggatcaagc ggaacatgga tgcgactcca
      781 ggccgggccc gcggtaccgg gaagcccggc gtcccggccc cgggggccgg aagctatcgt
      841 ccggcgggaa cgcggaccag cggcagcgcc cgctcggtca gctcggcgag cgggagccgg
      901 ttcaggtcgg gcaccgtctg gaagatccgg atgcggcgct cgaccagctg gtcttcgcgc
      961 ttggcggcga agaacgtccc cttgttcgtg agcacgtcgc gctcgaagaa cgtcgggagc
     1021 gggtagtgac ggcgctcctg cgcggggatg tggttctcga gcaggtaacg gcgctccttc
     1081 acctcttcgg gaggcatcgg ctcgtcggct ggtaggggcg tcagcacctg gtagtcgcgc
     1141 agtgccagga actcggtcca gacgtcgagg ctggccacgc cgccattggg cgtcgtgtgg
     1201 aacgtgaaga agggactgat ctcccgcgac aggccggtgt cctcgcccag gatcagcgcg
     1261 agccgggtca ggcgggagag cccgcagatt ccgcgcacct cggggacggc ccgggtgtcg
     1321 gagaaccagt agagcagcgc gagaaagtcc gcccggctga gcggaccgcg cgccgagatg
     1381 tcggcttgcg ggattccggt gctcacgtac gcccctcctc tagctcgatg cgcgcgcccg
     1441 cggcggcccc acgtcgatgt tgccgttcgt ggtctgcagc tcgatccgga cgccaccgcc
     1501 cccgatcgta ccccgaatcc tcttcgagtt gatcgtccct tgagtcgtaa gcttgtaatt
     1561 gatgttcacg cgcccgtttg tggtctccgc ctccagttcg gcgtgaaggt cgcgcggaag
     1621 cgcaagctcg atatttccgt tcgtcgtggt catctccatc cggccgcgat cgggcagcgc
     1681 cgccatctcg gccgcgatgc tcccgttcgt ggtcgaagcg ttcgtcgccc cctcgatccg
     1741 ggcgaggcga acctctccgt tcctcgtgct cgccacgagc gttccccgaa cgccggccac
     1801 gtccacgtcg ccgttgttcg tggccaccgc gaccctcccc tgcaccgcgc gcacgcggat
     1861 cgccccgttc gaggtgttca ggtcgagcga agcctgtcgc ggcacctgaa ggtagtagtg
     1921 gatgttcatc gactgcacgc catggccgat caggtcccag aagcccacca ccttctgctt
     1981 cctcggaaag acgctctcga tccggatctc ccctggatgc acggcgacgt cggccttgag
     2041 ctcgcgcaag agggccttcg ccatctcgtc agtgctggcg cgcacctcgc gtgtgatctg
     2101 cacccggacg tccgagcggt cccaggcttc gacgacgatc cggccgttcg tgctctcgat
     2161 gacgatgtcg ccgccggccg cgaacgggaa caccttctcg ctcacgttgc gcaggtgcgc
     2221 ggcgcccgcc ggcgacgcga tcgcaagcgc cgccagggcg caggcgccca accccgcggc
     2281 cgcgaacttc atcctggatc tcatggccac cctcctggac cggtccgaac gctggaactc
     2341 cgcattctct ttacggatcg gaccttcgga aggttccgct ttcaacgctg agcgagtctt
     2401 cacgacccca cgctcagatg ctgaaacgga tattcacgac atcgccgtcc cggactctat
     2461 agtctcgccc ctccgtccgc aagaggccct gctcgcgcgc tttaggcatc ccgccgagct
     2521 tggccaggtc gtcgtaggcg atcacttcgg cccggatgaa cccgcgctcc aggtcgctgt
     2581 ggatggcgcc ggccgcctcg ggggccaggg agccgtcccg gaccgtccag gcccggacct
     2641 cgtcgggacc caccgtgaag aaggagatga gcccgagcgt ggcgtacgaa aggcggatca
     2701 tccgcgtcag tcccggctcc tggatgccca gtacggccat gaactcgctg gcgtcctcgt
     2761 cggaaagctg gaccagctcc gcctcggtct tggcggaaag ggcgatcacc gggggcgcct
     2821 cggcgccgaa gcgcgaggcg accggcgcga cgagcgacgc ctccccctcc ttcagccgcg
     2881 cgtcgtccgc gttcaccacg acgatccaag gccgcatgct gagcagctgg aatccgcgaa
     2941 gcgcgcgctc gccggccttg gagagcccgg ccatgcgggc agggcgttcg ctctcgagcg
     3001 ccgcgcgcac cgctttcagc gcgtcgtact cgttcggatc ctcgggcttc ttccccacct
     3061 tgaccgcctt ctcgatccgc tccagccgcc gctccaccaa ggccaggtcc tccaggatca
     3121 gctcgctcag aagccacccc gcgtcgcgcg cggggtccac gcttccctgg aagtgcggga
     3181 cggccggatc gtcgaaggcg cggaggacga gcacgaaggc gtccacgtcc cggagcgcgg
     3241 cgagcgccgc tagcgcgccg cgctcggcgc gccccgtctc cagcggcggc gcgtccacgt
     3301 agtcgacctt ggccggcgtg aacttcttcg gctggtacat ctcgcggagg tgctcgaggc
     3361 gggcgtccgg cacccggacc gaacccaggt gcaccgtctc gccgcgcgcg gagccggcct
     3421 gggcctgtcc gcgcgtgagc gcggcgaaga gcgtggtctt cccgctctgg ggcggaccca
     3481 ggattccgag cttcatgggt tcagggtgtg ggtggcgccc cggtggggat cggccgggtc
     3541 aggcgccttc gccgcgctgg atcttgacgc tgagcgtgtc ggcctgttcc ctggcccacg
     3601 ccgcgtcctc gtccgtcggg aagcgcttcg cgtagtcgag gtagatcgtc cgcgcctggg
     3661 ccagcttcat gtcgcggcgc agagcgccgg ccttttccag aatggcggac ttgtacatgt
     3721 cggagtcggg gtagcggtcg atgatctgct gggcataccg ggcgtcctga ccgtagtcct
     3781 tgtccttgcg gctcattctc gcgaggctgt agagcgcgtc gtccaccagg cccgaccggt
     3841 tactggggtc catggcgacg agcttcttgt agcgcattcg cgcctcgtcg tagaggccgt
     3901 ggtagagata ccgctccgcc agccggccca cgtaacctgg atccttgccg ctgtccggct
     3961 ccatggccgc gtacgacgcg agcgtgttgc gtccggccag gtagtcctcc acctggtcca
     4021 tgaaggggcg cgcacggtaa tacccgacca cgcgatcgag ctcggtcccg tccttcttca
     4081 ggaccatgat cgtgggatag ctcttcacgc gatagtgagc cgcggtgacg gtgtccttct
     4141 ccgcgttcag cttcaccatc gtgaagcgct tcgagtattg gatgaaccgg ggatcagtgt
     4201 aggtagactc ttccagagcc ttacaccact gtcaccagtc ggtataaaag tccaggagaa
     4261 tgggccggcc ggtcttcccg gcgtcggcga gcgccgcgtc gtaggacgtg aaccacgcga
     4321 tcgactgcgg cgctgccgcc tcgtgcttct gctgggcgca cccgatcgcg agcgtcaacg
     4381 cgaacgctcc cgccattccc cagcccttca gggcgctggt cttcatgagc ctccttcgcg
     4441 atcggctcca ggacggagcc gaaggtgaat cccgatcatc ggttccgcct ccccgggttg
     4501 tcaaggtatc atcgggtgtc acccctaaag gaggcgggca tgagcgcgtg tcatttctgc
     4561 gggacgtccc tcgatccgaa gatgcggatc gtcaaggaca cggtttgccc cagctgcggg
     4621 cgcgacctgc acgcctgcgt ccagtgccgc cactacgacc ggaacgccca caaccagtgc
     4681 cgcgagccgc aggccgagtg ggtcaccgac cgggagcgcc gcaacttttg cgactactac
     4741 gccctgaatc ctgtgggcgg aaagggtgcc ggcgtgcccg atcgcgagaa ggcggcccgc
     4801 tccaagctgg acagcttctt tgcccccgga ccggccaagc ccggcccggc cgaagcccgc
     4861 tccaagctcg attccctctt caagcccgcc cccggcaagg acccggagcc cgacgacgaa
     4921 tgaagaaaat cgacgttctc gtgttcggcc cccatcccga cgacgcggag atcggagccg
     4981 gaggcctcct gctgaagatg aaggcgctcg gccacaccac cggaatcatc gacatgaccc
     5041 gcggcgagat gggctggggc acgcccgagc agcgcggcgc cgaatgcgac gaggcgtgcc
     5101 agatcttgaa gctcgacgtc cgcgagaacc tggacatggg cgaccaccgg atcgaggaca
     5161 ccttcgagaa tcgctgcacg gtcgccgcgg tcatccggaa gcaccgtccc gatctgatct
     5221 ttgcgccgca ctacgccatc ccgcccgggc gggggctggg ccacaacgac catttcaaga
     5281 ccggcatcct catcgcgcag ggccacaacc tcgcccattt gcgcaaggcg ccgctctccg
     5341 gcgagccgca ccaggccaag gcgatcttct actacttcct cccgcccggc gtgcgtccca
     5401 ccatcgtcgt ggacgtgacc ccgtacttcg aggactggat gcgcgcgctc tcctgccaca
     5461 agacgcaatt ctccaatccc gacaagccga agccgaaggg ggagcagttc tccgtcgtcg
     5521 agcgggtgga gacgatcgcg aagagcaacg cgtggctgat cggcgggaag tacgcccaga
     5581 cgttctattc ggacgcgccc ctcaagatcg gcgatccgat gcacctggtg caggagatcg
     5641 agcccaggcc gtgaggagga tctgagttag ggcttggcgt tcgtcccttt ttcgagcgcc
     5701 agtcccagct cggcctgcgc cggatccagc accacgacgt cgccggacct caactgctcc
     5761 atcgcgcgct cggccgagcg gcgatcctcg gggcgatgct tgagctgcca gagacggaag
     5821 aagagccatc ccgcatcgag gtttccctgc tccggcttct cccccgccgc caggcggccg
     5881 atggcctgtg aatcgcgcgg cgcataacgg aatccccctg ctaccggatc ctcgaggtgc
     5941 gtccggatcc agaccgcgat cgattccgcg cgcgccaggt gacgaggctc cttggtggcg
     6001 gcgtaggcct cgagataggc gagcccggcg gcggcctggt ccatgagccg ccccggcacg
     6061 gccacccgcc cctcgcgcca ttcgtggtag agcgatccgt ccggcgcgcg ctgcgcggtc
     6121 catagccggt ccagcgcctt caccgcccgc gcgaccggcg cgcccttcca gatgcccgcg
     6181 gccgctccat gcaacacagc cgccgccatg cgcgcgttct gctcggcgta cagcgacgag
     6241 tcgatcgcgg gcaagccctg cttgcgccgg agcgcgtcgg acaggccgta gtagacgtca
     6301 cctccgagca tgcgttcgcc gcggtcgggt tcgccggagt cgtggccggg tccgccgccg
     6361 tctcggccgg gtccgccggc gtcgcggtct tgcgagggtc tgtggccctg atggagcaca
     6421 acgtccgccg cctggctgcc gtaccacccg cccgggcgcg gcgcccagag ccacgtggcg
     6481 gcgtaggcct cggtgcgacg cgcggcgtcg cgataccgcg tgccgccccc ctgctccacc
     6541 gccccgacca gcgccgcggt cgcgcgcgca ttcacgtcga gaagcttctc ggtccgcggc
     6601 cttttccagt cctcgtcgcc cgcgatccgg aagaagccgc cccaggtcgt gtcctcgaga
     6661 tggagcgcgg cgtcgagcac gcgcaggccc agcgagcgga gcgcgcgatc gccgcgcgcc
     6721 gccgaggccg agagaagaaa gtgcacagcc tcgaactgtg gctgcttcgg cgacccctcg
     6781 atgccgccgt gcacatggtc ctcggcctcg cgcagggcgg ccaggttccg gtccacccag
     6841 tcctccagcg tcatgtcggg cggaaccaga ggctcgctct tccaggtgtg ctcgatgcgc
     6901 cgcgccgact cgccggcgcg gaactcgatc tcgctccggt tctgccggta gaggtcgccc
     6961 acccgctcca gcatctgccg taggggttgc ggcgcgacga ccgattgcgc catgagcacc
     7021 tggccgtcgt ggctgagcac ggccgtggtc ggccatcccc cggagagata gcggtcggcg
     7081 atgtcaggcc tgcgatcggc gtccacgcga acggggatga agccctggtc cacgacccgc
     7141 acgacagacg tgtcggtcca ggtctcgaaa tccatctgac gtcccgagcg cgaccaggag
     7201 gcggcgatat gcaggaggac cagcttcttc tcggcgcgcg cttcctcgaa cgcggcgcgg
     7261 tcccatgggc gccaggcgat aacgcccgca gcggtgtggg ccggcgcggg ctgcgctggg
     7321 gcggatggag gcgtcgcgga tggcgctgcc gcggatgggg ctgccgtcga cggcgcggcg
     7381 gcgagggcga tcaggagaac cgcgaacgtc agaagcggga tgcggccttg gcggtcttgg
     7441 cggcggtctt cgcggcggcc ttggcctgcc ggctggcctt ggagcgcgcc tcgcgctcgt
     7501 tcaggaaccg ctccaggtcc ttctcgtcgc gcaggaggat cttctcggcg gggtcgggac
     7561 aatagcgctc gcagtcggcc gggtcgtaca cgcggcagac ctccgggcga tgcacgtagt
     7621 ggctgcaccg gccgtccttc agccagtcgc agggcgtgtc gaactgcacc atccatgagc
     7681 cttcccaatc gcacaggacg gagacgttcc ggtggaggag gtaccatcga atgttatcga
     7741 aatcccggcg cgagcgcggc cggtcgatgg gaatggtaac gtaacggcag caatgatcgc
     7801 acccctcgca cggattccct tccggaagct ggaagcagcc gttcgccagg agcggcaggt
     7861 tcagcgccgg ccgcggcggc cgcatcggcc cgcgccccgg gcgctgctct ccgtcggcgt
     7921 ccgggtggcc cgtcgcgtgc ggctttgcgc tatgttcgtc gtctcgtccg ctcacgccgc
     7981 gccctctcgt cccctgtcgg tgccgaaggt cgcagcataa gcgcaggact ccatggaggc
     8041 aaacggcggg tgaagaaatc gaaccgtctg gttggccgcc gcaacgagga tgccgtcgcg
     8101 tgggaagctc gagcccgcga ggcatcgccc cgctcctagt cattccatcg gataccggcc
     8161 gttcgcttcc ctgcgggata ccgttagtac cagtaggcgg cggagagacc tccgcgtgcg
     8221 tctggaaatc ccatcaaggg ggacaggtca tgaatcggcg ttggagcttt acgcctaagc
     8281 tt
//
LOCUS       DASSLZ010000312         4590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1737029, whole genome shotgun sequence.
ACCESSION   DASSLZ010000312 DASSLZ010000000
VERSION     DASSLZ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737029"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /gene="pheT"
                     /locus_tag="VFM00_11760"
     CDS             complement(<1..1335)
                     /gene="pheT"
                     /locus_tag="VFM00_11760"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7498966.1"
                     /translation="MKVTWSWLGDWTGLPESPEALAHLLATRGLPVESLEKGTAYDPT
                     IVVGHVLEVAPHPNADRLRLCKVDLGGAVRSIVCGASNVAAGQRVAVAQIGSKLPDGT
                     KLKKAKIRGVESEGMICSEKELGLSAESEGIWVLPGSPVVGMPLTDLLGSGDAVLDVE
                     ITSNRTDCMAVRGLARDVAAARGERLKDPPALRATGKGDLPRVTIENAKDCPRYMARV
                     VTELRIGPSPDWLRRRLEATGFRSINNVVDVTNYVLREYGQPIHAFDAAKVGGREIRV
                     RRARSGESLTLLDGRKVALGPHVQVIADATIPMALAGIMGGLDSGVTDATTSVVLESA
                     QFDPGLTKEAARSLGVETDASTRFGQGVDPDGVALALDATARLLAEVAEGTVVDAVVD
                     QWPGKGEPRAITLRLDRLSRLLGIPVAPETATRALAGLEIAQQGAWRREGDET"
     gene            complement(1379..2386)
                     /gene="pheS"
                     /locus_tag="VFM00_11765"
     CDS             complement(1379..2386)
                     /gene="pheS"
                     /locus_tag="VFM00_11765"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010209123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET7498967.1"
                     /translation="MERIEALGVEGERALREAASAAELEAARVRYLGRKSDLTQVLRG
                     LKDLAPEARAQVGARANATRVRLEAALAETEARLAAQAPAAARDVTLPGRRAPVGHRH
                     LITSVFREIEQIFGGLGFSVATGPDVEDDWHSFTALNMPEGHPARDETDTFYLQSGAL
                     LRPHTSPVQIRTMETNPPPVRIICPGRVYRNEAIDATHQVEFHQVEGLYVDRNVSLAD
                     LKGTLDHFLKAFFGRDTEIRFVPSYYPFVEPGAAVDIRCFFCGGKGCNVCGPLSGGWL
                     EVLGAGMVHPNVFRAVGYDPEVWTGWAFGAGIERLVMLRHGVPDIRMFLESDVRFLRQ
                     F"
     gene            complement(2421..2783)
                     /gene="rplT"
                     /locus_tag="VFM00_11770"
     CDS             complement(2421..2783)
                     /gene="rplT"
                     /locus_tag="VFM00_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET7498968.1"
                     /translation="MPRTKTVVPGRKRRRKVLTAAKGNRGGRRKLYQTARETLLRGMQ
                     FAFRDRRAKKRDFRRLWIVRINAAARLHGLSYNEFIRGLKVAQVGIDRKVLADLAVRD
                     AAAFARLAEVAKQAQAAA"
     gene            complement(2821..3021)
                     /gene="rpmI"
                     /locus_tag="VFM00_11775"
     CDS             complement(2821..3021)
                     /gene="rpmI"
                     /locus_tag="VFM00_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET7498969.1"
                     /translation="MPKMKTSRATAKRFKHTGTGKIKRSRAYSRHHLGLKTRKQKRRL
                     HSSAIVEPTEKRHMQKLLPYGS"
     gene            complement(3051..3761)
                     /gene="infC"
                     /locus_tag="VFM00_11780"
     CDS             complement(3051..3761)
                     /gene="infC"
                     /locus_tag="VFM00_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014983430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET7498970.1"
                     /translation="MATTKEIRVNDRIRIPSVRVIGPDGEQIGILAIREALAYAQERN
                     LDLVEVSPTSKPPVCRVMDFGKYKYEQNKKAQKARKKQHVTHLKEVKLRPKIEDHDYN
                     FKVEHGRQFLLEHDKVKFTVMFRGREMAHPEAGFRLLQKVVKDLEEVGMVEIPARMEG
                     RSMTLLMVPRQAALKPGEKKPATAGTATSATSSAPRPATASGAGTRPAASSTGPKPAS
                     PAPRPASASSPAEVKPKS"
     gene            complement(3971..>4590)
                     /locus_tag="VFM00_11785"
     CDS             complement(3971..>4590)
                     /locus_tag="VFM00_11785"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET7498971.1"
                     /translation="HEWELAENALENVVKRAGLPYHRAEGEAVFYGPKIDIKLTDAIG
                     REWQCSTIQFDFNLPRRFEVGYVAADGQEHQAYMVHRALLGSMERFFATLIEHYGGDF
                     PVWLAPVQVQLITITDDQKPYAEEVKALLREQGIRAEADLREEKMGAKIRDAELMKIP
                     YMLVLGKKEVEDRAVSVRSRKKGNEGSLPVAAFVEKVVAEVRART"
BASE COUNT          619 a         1578 c         1597 g          796 t
ORIGIN      
        1 ggtctcgtcg ccctcgcgcc tccaggcgcc ctgctgcgcg atctcgaggc cggcgagcgc
       61 gcgggtggcc gtctcggggg cgaccggaat gccgagaagc cggctcaggc ggtccagccg
      121 gagcgtgatc gcgcggggct cgcccttgcc cggccactgg tccacgaccg cgtccacgac
      181 ggtcccctcc gccacctcgg cgaggagccg cgcggtcgcg tccagcgcga gcgccacgcc
      241 gtcgggatcc acgccctggc cgaaccgggt cgacgcgtcg gtctcgacgc ccagggatcg
      301 cgccgcttcc ttcgtgaggc cgggatcgaa ctgggccgat tcgagcacca ccgacgtcgt
      361 cgcgtcggtc acgcccgagt cgagaccgcc catgatgccc gcgagcgcca tcgggatcgt
      421 ggcgtcggcg atgacctgca cgtgcgggcc gagggcgacc ttccgcccgt cgagcagcgt
      481 gagcgattcg ccgcttcgcg cgcggcgcac acggatctcc cgtcccccga ccttcgcggc
      541 gtcgaacgcg tggatcggct ggccgtactc gcgcagcacg tagttcgtga cgtcgacgac
      601 gttgttgatg ctccggaatc cggtcgcttc caggcggcgg cggagccagt cgggcgaggg
      661 gccgatccgc agttccgtga cgacgcgcgc catgtagcgg gggcagtcct tcgcgttctc
      721 gatcgtgacg cgcggcaggt cgcccttccc cgtggcacgg agcgccggag gatccttgag
      781 ccgctccccg cgcgccgcgg cgacatcgcg cgccaggccg cgcaccgcca tgcagtcggt
      841 ccggttcgag gtgatctcca cgtcgagcac ggcatccccg gatccgagga ggtcggtgag
      901 cggcatgccg acgacgggcg atcccgggag cacccagatc ccctccgact cggcggaaag
      961 gcccagctcc ttctcggagc agatcatccc ctcggactcg acgccgcgga tcttcgcctt
     1021 cttgagcttg gtgccgtcgg gaagcttgct gccgatctgc gccaccgcga cgcgctgccc
     1081 ggcggcgacg ttcgaagcgc cgcagacgat cgagcgcacg gcgccgccca ggtccacctt
     1141 gcagaggcgc agccggtcgg cattgggatg cggcgctacc tcgagcacgt gccccacgac
     1201 gatcgtggga tcgtaggccg ttcccttctc gagggactcg acggggagcc cgcgcgtggc
     1261 gagcaggtgc gccagcgctt ccggggattc cggaaggccg gtccagtcgc cgagccagct
     1321 ccaggtgact ttcatcgggt ttcgctccgc atcgcgttca gggtgtcggg atcgcgcctc
     1381 agaactgccg gaggaagcgc acgtcgctct cgaggaacat ccggatgtcg ggaacgccgt
     1441 gccgcagcat gaccaggcgc tcgatgcccg cgccgaacgc ccagcccgtc cagacttcgg
     1501 ggtcatagcc gaccgcgcgg aagacgttcg gatggaccat gcccgcgccc agtacctcca
     1561 gccatccgcc cgagagcggg ccgcagacgt tgcatccctt cccgccgcag aagaagcagc
     1621 ggatgtccac cgcggccccc ggctcgacga acgggtagta cgagggcacg aagcggatct
     1681 cggtgtcgcg gccgaagaac gccttcagga agtggtcgag cgtgcccttg agatcggcga
     1741 gcgaaacgtt ccggtccacg tagagcccct ccacctggtg gaactccacc tggtgggtgg
     1801 cgtcgatcgc ctcgttccgg tagacgcgcc ccgggcagat gatgcgcacg gggggcgggt
     1861 tcgtctccat ggtgcggatc tggaccggcg aggtgtgcgg ccggagcagg gcgccgctct
     1921 ggagatagaa cgtgtcggtc tcgtcccgcg cgggatgccc ctcgggcatg ttgagggcgg
     1981 tgaagctgtg ccagtcgtcc tcgacgtcgg gaccggtcgc gaccgagaag ccgaggcccc
     2041 cgaagatctg ctcgatctcg cggaagacgg aggtgatgag gtggcggtgg ccgaccggcg
     2101 cgcggcgtcc cggcagcgtc acgtcgcgcg cggcggcggg agcctgggcg gcgaggcgcg
     2161 cttcggtttc ggcgagcgcg gcctccagcc ggacccgcgt cgcgttggcg cgcgcgccca
     2221 cttgggcgcg cgcttcgggc gcgaggtctt tcagtccacg gagaacctgg gtgaggtcgc
     2281 tcttccgccc caggtagcgg acgcgcgcgg cttccagctc ggccgccgag gcggcctcgc
     2341 ggagcgcgcg ctccccctcc acgccgagcg cttcgatccg ctccatcatc ggcacggact
     2401 cggagacggg cacgacgggg ttaggctgcg gcttgggctt gcttggccac ctcggccagg
     2461 cgggcgaagg cagcggcgtc ccggacggcg aggtccgcga gcaccttgcg gtcgattccg
     2521 acctgagcca ccttcaggcc ccggatgaat tcgttgtagg agaggccgtg gagacgcgcg
     2581 gcggcgttga tgcgcacgat ccacagccgt cgaaagtccc gcttcttcgc cctccggtcc
     2641 cggaacgcga actgcatgcc gcggagcagc gtttcgcgcg cggtctggta gagcttgcgg
     2701 cgtccgcccc ggttcccttt cgccgcggtc aggaccttcc ggcggcgctt gcgacccggt
     2761 acgacggttt tggtgcgtgg catcgttcag ttcctcatct tgtggccgat ccgttcggga
     2821 tcagctgccg tagggtagaa gcttctgcat gtggcgcttc tccgtcggct cgacgatcgc
     2881 cgaggagtgg agccggcgct tctgcttgcg ggtcttgaga cccaggtggt gccgcgaata
     2941 ggcgcgggac cgcttgatct tgccggtgcc ggtgtgcttg aagcgcttcg ccgtggcccg
     3001 gctggtcttc atcttgggca ttcggactcc ttcgtgggtc tcgtgaaccg ctacgacttc
     3061 ggcttcactt cggccggcga cgatgccgac gccggtctcg gcgcgggcga tgccggtttc
     3121 ggccccgtac tcgacgcggc cggcctcgtg ccggcgccgc tggccgtcgc gggacgcggc
     3181 gcgctgctcg ttgccgacgt cgccgttccg gccgtggccg gcttcttctc gcccggcttg
     3241 agcgcggcct ggcgcggcac catgagcagg gtcatgctgc gcccctccat gcgcgccggg
     3301 atctcgacca tgccgacttc ctcgagatcc ttgaccacct tctgcagcag gcggaacccc
     3361 gcctccggat gagccatctc gcggccccgg aacatgacgg tgaacttcac cttgtcgtgc
     3421 tccagcagaa actgccggcc gtgctcgacc ttgaagttgt agtcgtggtc ctcgatcttc
     3481 ggccggagct tcacttcctt caggtgcgtg acgtgctgct tcttccgcgc cttctgggcc
     3541 ttcttgttct gctcgtactt gtatttgccg aagtccatga cccggcagac cgggggcttg
     3601 gacgtgggcg acacttcgac gagatcgaga ttccgttcct gggcgtaggc gagcgcttcg
     3661 cggatggcca ggatgccgat ctgctctccg tcaggtccga tcacccggac cgacgggatg
     3721 cggatgcggt cgttgacgcg aatttccttc gtggtggcga tcgagcgttc ctccttattc
     3781 gtcctgaatg ccttggtctc ggcgcggggg agcgttgctg gcgggggtag ccggagacgc
     3841 gtccatgacg cgcgtcggcg gcagtgcgtc ggagggcccg atgaccctgc gacggcggaa
     3901 accccggcag ctcggcgccc cggggtgaga agccagcagg ctcctactta acaccgaatg
     3961 ggagagcagt ttaggttctg gcgcgcacct ccgcaaccac tttttcgacg aaggcggcga
     4021 cgggcaggga cccctcgttc cccttcttgc ggctgcggac ggagaccgcg cggtcctcca
     4081 cttccttctt tcccaatacc agcatgtagg gaatcttcat cagctcggcg tcccggatct
     4141 tggcgcccat cttctcttcc cggaggtcgg cctcggcccg gatcccctgc tcgcgcagga
     4201 gcgccttgac ctcctcggcg tagggcttct ggtcgtcggt gatcgtgatc agctgcacct
     4261 ggaccggcgc gagccagacc gggaagtccc cgccgtagtg ctcgatcagg gtggcgaaga
     4321 agcgctccat ggagccgagc agcgcccggt gcaccatgta ggcttggtgc tcctggccgt
     4381 cggcggcgac gtaccccacc tcgaagcggc gcggcaggtt gaaatcgaac tggatggtcg
     4441 agcactgcca ctcgcggccg atggcgtcgg tcagcttgat gtcgatcttg ggaccgtaga
     4501 agaccgcctc cccctcggcg cggtggtacg gcaggcccgc gcgcttcacg acgttctcca
     4561 gcgcgttctc ggccagctcc cactcgtggt
//
LOCUS       DASSLZ010000313        14094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1637146, whole genome shotgun sequence.
ACCESSION   DASSLZ010000313 DASSLZ010000000
VERSION     DASSLZ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14094
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637146"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1840
                     /locus_tag="VFM00_11790"
     CDS             <1..1840
                     /locus_tag="VFM00_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7498972.1"
                     /translation="RVAPSRAEAPHGDTYGPAGWTAGTPAYMAPECLRGEAPDRRCDI
                     WALGVVLYEMLAGTRPFVADTRADLERAIRQEDPAPLPRAIPEGLRRIVDRCLEKDPG
                     RRYQRAGEARAALEAVRDARRQSLRWNRWRVGAAAAAAIAIAAVAVYAVLHLPHERTV
                     LVLPAHNAAGDSAQQYLADGLTDDLINTLMRLSSVRVLSRHSSWVIAREQVPLPTLAR
                     RHGVDYALESSIVQIGEHERVNVALVNAASDHPIWARAYERPLRETQTLDRDIVRGVA
                     DALKLSLSPFERAMLGPAPPVNSDAYEQYVLGRNFAAERSYRRALACYAEATGLDGTF
                     AAAHAARARCLVEMLYYRVAPPESLPPAQASANRALAIDSTLASAHLSLAYVHGFLWD
                     WDGARGELERASEDEPGSADVACGWSFYYGVLGRAREEVDAMERAVKSDPLQVRYRNE
                     LGLAYLNAGRVGDAEKQFAIAERSAPSEESHWAAAYRARCLALRGKTAEATTILERLA
                     RDGQAVYAREELAYVLARAGRRAEASRLVAELAAMPAGAVSPLAIAAAEIALGRPESA
                     LTVLERACDQHESRILWIRVDQRFDPIRKDPRYANLLERMMSASAT"
     gene            complement(1843..2208)
                     /locus_tag="VFM00_11795"
     CDS             complement(1843..2208)
                     /locus_tag="VFM00_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498973.1"
                     /translation="MKQTDDYLLRQIQTVAAMIARAAGLRLEGSPDEARAVLEQAYGV
                     LLAGQTTLIRRLDAATAASLLGSPERTLMLARLLEAESGLEADTALHERAIALAREAL
                     RRDPENDEAKAMLGGLSAP"
     gene            complement(2219..3157)
                     /gene="egtD"
                     /locus_tag="VFM00_11800"
     CDS             complement(2219..3157)
                     /gene="egtD"
                     /locus_tag="VFM00_11800"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET7498974.1"
                     /translation="MDPELLAAITAGLSRPGQKKLPAALLYDELGSALFEAISALPEY
                     GLTRADERILRRHAPAITRGLATPLLVAELGSGTGRKTRWILDALASRQTTHYYPIDI
                     AETALAKCATVLGRLPAVRFEAIHADYLAGLANVAAQRRSAHHLLVLFLGSTIGNFEP
                     DAARSFLREVRRTLKPGDALLLGTDRVKPAPTLIRAYDDPLGVTAAFDRNLLTRMNRD
                     LGASFDLSLFRHEARWREAERRIEMHLVAKRAHEVAIPALDLRVAFKDGESIWTESSY
                     KFEKDDVAKLAKAAGFEVAAEWVDEEWPFAETLLRV"
     gene            complement(3229..4548)
                     /locus_tag="VFM00_11805"
     CDS             complement(3229..4548)
                     /locus_tag="VFM00_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET7498975.1"
                     /translation="MTQTLRSSETPEALAARLTGARAQTDQIFEVLKPEALYERAIPE
                     RHRLAFYLGHLEAFDGNLLRQAYDLASFNPVYDKLFAFGIDPVDGQLPSDAADVWPRP
                     EPIRAYNGRIREAVDECLTGKRPIGAGQDAGTIINMAIEHRLMHAETLAYLLHQLPLK
                     LKKGKPDAPIPSATGLDASRTVRVPAGKATLGRSRSAGVFGWDNEFGERVVQVPEFEI
                     DAYKVTNGRFLEFVKDGGYEDPIHWTEENWEWRERQKLVHPPFWTRKAGAWHHRGMFS
                     EPLLSMDAPVYVSHAEASAFARWAGRSLPTEAQFHRAAYGTPSGEEREYPWGNQPPGE
                     EHGNFDFHRWDPTPVGGHPAGASAFGVDELVGNGWEWTSSLFEPFPGFEAHPFYRGYS
                     ADFFDGKHYVMKGGSPRTAACMLRRSFRNWFQPKYPYVYGAFRTVAG"
     gene            complement(4514..6139)
                     /locus_tag="VFM00_11810"
     CDS             complement(4514..6139)
                     /locus_tag="VFM00_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7498976.1"
                     /translation="MDVRPGVAWLLAAALAGAAAPGPARAERGVAVGSKAFAESWILG
                     DALTAAMREAGVSDAAHRRNLGGTEIVLQALRSGNVDVYPEYTGTVQEVVLHAPRPLS
                     PPEMRAALARIGLGMSEPIGFDDSYAIAVTPEAARRFGLRALSDLAGHPEVRLGLTHE
                     FLGRADGFPGLAARYGLSRKNVTGIQHELAYAAMASGKIDATDIYTTDAQIERMHLVV
                     LRDDRAFFPRYDAVWLYRLDLARREPRAMAAMRRLTGTIDQARMIRANARVVLGGAES
                     AAVAESLLAEISGPGAPAASSASEGRAARIAKNTVQHLKLVLLSLLAAVLIGVPLGVA
                     AARSRWIGGPVLAATGLAQTIPSLALLAVLIPLLGIGILPALVALFLYSLLPIVRNTY
                     VGLTTIPPPLIESAACLPLSARAQLTRIRLPLASPAILAGIRTSAVINVGSATLAALI
                     GAGGLGEPILSGIQLRRNDLILEGAIPAALLALLVERAFDLADRMAVPKGLRLATGAG
                     AKRGRRGADSSKIEGESTLEAHHDPDAPIERNP"
     gene            complement(6130..6900)
                     /locus_tag="VFM00_11815"
     CDS             complement(6130..6900)
                     /locus_tag="VFM00_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7498977.1"
                     /translation="MPDPPDRPDPADPVELEGVVKKFGDATALDVPHLAVGERTTLAV
                     VGPSGCGKSTLLRLVVGLLVPDRGVVRVAGAVMGPSTRPAVLPRIGYVIQEGGLFPHL
                     TAGANVTIAAEDRGWSRERREKRLLELLELTQMPRALLARYPAELSGGQRQRVALMRA
                     LMLDPEVVLLDEPLAALDPMIRADLQRDLRAVFERLHKTVLFVTHDVAEAATIGDEIA
                     LMRAGRVVQRGAFRDLIDRPADPFVTAFLTAQRAVAWT"
     gene            6943..8904
                     /locus_tag="VFM00_11820"
     CDS             6943..8904
                     /locus_tag="VFM00_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498978.1"
                     /translation="MRDSFQPARLLSLVGPTVLLPALVAAFLAAPGLSFDYFWDDFFF
                     LTKAQGSPTAYLVPNAAEAFWRPIPQGLYFRLLLALGPAGPFAGHFLNLLLLAAAAML
                     LASLASRLAGPRAGLLAGLVFAASAALPSLVAWTSACQDLMAMVFLLIAFHLRESGKI
                     AGSAGVAALALLSKETAAVALPVLVAWDWLLGREPARLVRGALLFGIVTAAWFAVHPG
                     LRALVHAGLQSGTTGYVGLERPERWWEYLFRYVATLFNFPVNAVSWPARLTVAGIAAA
                     VALLVTAPGALQRLKPADATSSDDPGRGTAPRPRPTARRLQIAGLLIALPPILLPSLL
                     VRPWATYFAALAVPGFALLAAMRLARLTPRTAAIAIAAFLLLGVWARGMDARGNGRVL
                     TEQRFAEASLATRRVRERFRKIVPEIPSGARVLLSVAGTGTLGIHQTLQDGQALRHWY
                     RDPTIATVQPEWKRPPGADEFLVRVTPDLQVIQIDPTTSRFRTEGDDPDREEIAKPIR
                     SYARGLAASGATDPAVWILERMATADSTRLRSYDLRLAALALHAVHRDGEAGHLLRLA
                     PPLQRWEALDMAGKVYAEPTHDPAHDALAFWPFGVSPDDPGALRYLIGQYRIGGYYRQ
                     ARELARRLERLAPGDSLALATIRFNPLRE"
     gene            8910..10448
                     /locus_tag="VFM00_11825"
     CDS             8910..10448
                     /locus_tag="VFM00_11825"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET7498979.1"
                     /translation="MLLAELASTSREVAATGARSVKAERLAALLRRLEGDEIEIGVAW
                     LSGHPRQSRLGIGGATVHGVRGSEAAAAPSLTLADADTALARIAALAGAGSGGERQRS
                     LAALFARATAEEQDFLARLIVGELRQGALAGVMEDALAKATGIPAAEIRRATMLSGDA
                     EAVARAVLHEGRAALARFQVELFRPVQPMLASPADDLDAALERLGEAALEYKLDGARV
                     QLHKSGEEVRVFSRRMNDVTVAVPELVEAARALPAREMILDGEAIALREDGRPHPFQA
                     TMRRFGRRLDVEALRGELPLTPFFFDLLRLDGADFFDVPARDRFEALARVAPALVVPR
                     RVTGDVDAAEAFLREALARGHEGLMAKAPEARYEAGRRGFSWLKVKEAHTLDLVVLAA
                     EWGHGRRKGFLSNIHLGARDAASGGFVMLGKTFKGMTDAMLAWQTQRFQELAVATDGY
                     TVQLRPELVVEVAFNDVQESPHYPAGLALRFARVKRYRPDKAAAQADTLETVRAIHEG
                     RLSS"
     gene            10484..11344
                     /locus_tag="VFM00_11830"
     CDS             10484..11344
                     /locus_tag="VFM00_11830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET7498980.1"
                     /translation="MGFLGLFWIAFQDWRRDRADRLGAALAYYSILSIPPLLLLLAGI
                     AGLVFDPQSVRGRLLATLQQLFGAQGTSAVRDILAGMDRNGHSGTATLVGFGTLLLGA
                     SGVFGHLKDSLDTIWNVRQDKGSGWKGWLKDNVFSIVVLMGAAFLLVISLAVNALLAA
                     ASDYLSRNLPGGTTLWGGVNLLVSLAVLAALFGLMFRYIPAVKLRWRDAVIGGLVTAA
                     LFMAGEAAIGFYLGKTNVGSAFGVAGSLVVLLVWVYYSALIFFYGAEFTQAYAERRGT
                     RPAPRRERTP"
     gene            11413..12336
                     /locus_tag="VFM00_11835"
     CDS             11413..12336
                     /locus_tag="VFM00_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HET7498981.1"
                     /translation="MGHPIAILFLHGIGRTEPGYSRPLAEAIAARFTRSAGGETSNPE
                     AQIVFEEVNWSGALQPAEDDLGARLRAAGPLHFGRLRGFMLDFAADAIAYQPTHADRS
                     AYDAIHAEVAASLGRLARRAGARAPLGVIGHSLGSVIASNYVYDLTKPRGSFMAPEVR
                     ARIGKTPLERGETLSLFYTMGSPIALWSLRYPDFGVPVKLPVKRLAGHHPGLEPRWVN
                     FYDPDDVIGYPLKPLNEAYREAVDEDRAVDVGSWLTRWNPLSHTGYWGDAGVADVIAA
                     GVTEAWRRSSAIALVGAKKPRTRPRRTASRT"
     gene            12422..13258
                     /locus_tag="VFM00_11840"
     CDS             12422..13258
                     /locus_tag="VFM00_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET7498982.1"
                     /translation="MRRGRLLLGLLIAAISVISYYSLQQQNPVTGETQHIAMDVDQEV
                     ALGLESAPQMAQEMGGLDPDESIQQRVQRVGQRVVQQSEARGTPYQYHFYALADPQTV
                     NAFALPGGPVFITRGLLSRLENEAELAGVLGHEVGHVVGRHTAAAIAKSQLAQGLIGA
                     AGVAGSDDQGGGQRSAQLAALVAQMVQLRYGRGAETQSDTLGVRFMTEAGYDPRAMLE
                     VMRILSESSGPGREPEFMSTHPDPGNRSQVIQGAIERRYPNGIPSNLTLGERFETTYG
                     SR"
     gene            complement(13239..>14094)
                     /locus_tag="VFM00_11845"
     CDS             complement(13239..>14094)
                     /locus_tag="VFM00_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498983.1"
                     /translation="GAGGGGGSGAYAGGAGGAGGNLASLFVAAPGDTGSGPNGGAGGD
                     PGLVTGRKHNGTAGGTGGGGSPGTDGPGGVAVGGLGTSGYQPAAGADGGSDGTGGSGG
                     GGGGGASGSPTDGAGNGGGGGGGGGTPGAGALAGGGGGGSFAIAAVATTNLVVERCVL
                     RTNQGGAGGSGGSGGPGGGGGAGGAGGSAGKDCNSLIGNGGAGGAGGTGGRGGHGGGG
                     GGGPTIGILYDVASTLTESGNSYTLGTAGAGGPSPGNDGAAGARQNVLRLGGGAPAAA
                     ARVSATRR"
BASE COUNT         1863 a         5154 c         5031 g         2046 t
ORIGIN      
        1 ccgggtcgct ccctcccgcg cggaagcgcc gcacggggac acgtacggtc cggcgggatg
       61 gacggccggc accccggcct acatggctcc cgagtgcctg aggggggaag cccccgaccg
      121 gcgttgcgac atctgggcgc tcggcgtggt gctctacgag atgctggccg ggacgcgtcc
      181 gttcgtggcc gatacccgtg cggatctcga gcgggcgatc cgccaggagg acccggctcc
      241 gctcccgcgc gcgatccccg aggggttgcg gcggatcgtg gaccgttgcc tggagaagga
      301 tcccggccgg cgctatcagc gggcgggcga agcgcgcgcc gcgctcgagg ccgtccggga
      361 cgcgcggcgt cagtcgttgc ggtggaatcg gtggcgcgtc ggagccgccg ccgcggcggc
      421 gatcgcgatc gccgccgtgg ccgtctacgc tgtcctccac ctcccgcatg agcgcacggt
      481 gctggttctc cccgcgcaca acgcggccgg cgactccgcc cagcagtacc tggcggacgg
      541 cctcaccgac gatctcatca acaccctcat gagactctcg tcggttcgcg tgctgagccg
      601 ccactcgagc tgggtcatcg cccgggagca ggtcccgctg cccacgctgg cgcgccgcca
      661 cggggtggac tacgcgctcg agtcctcgat cgtgcaaatc ggggaacatg agagggtgaa
      721 cgtggcgctg gtgaatgccg cctcggacca tccgatctgg gcccgcgcct acgagcggcc
      781 gctgcgggag acgcagaccc tggaccgaga catcgttcgc ggcgtcgcgg acgcgctgaa
      841 gctgtcgctc agccccttcg agcgcgcgat gctggggccg gcgccccccg tgaacagcga
      901 tgcctacgaa cagtacgtgc tggggaggaa cttcgccgcc gagcggagct atcgccgggc
      961 gctcgcgtgc tacgccgagg cgaccggtct cgacggcacc tttgccgcgg cccacgccgc
     1021 gcgcgcgcgg tgcctggtcg agatgctcta ctaccgcgtg gcgccgcccg agtcgcttcc
     1081 gcccgcgcag gcgtccgcga accgcgccct cgcgatcgac tcgacgctgg cttcggcgca
     1141 cctctcgctc gcctacgtgc acggctttct ctgggactgg gacggggcga ggggggagct
     1201 cgagcgcgcc tcggaggacg agccggggag cgccgacgtc gcctgcggct ggtcgttcta
     1261 ctacggcgtg ctcggccgcg cgcgggagga agtggacgcc atggagcgcg ccgtgaagtc
     1321 cgatccgctt caggtgcgct atcgcaacga gctggggctc gcctacctga acgccgggcg
     1381 cgtcggcgac gccgagaaac agttcgcgat cgccgaacga agcgcgccct ccgaggagtc
     1441 ccattgggcg gcggcctacc gggcccgctg cctggcgctt cgagggaaga ccgcggaggc
     1501 gaccacgatc ctcgagcgcc tggcgaggga cggacaggcg gtctacgcgc gcgaggagct
     1561 ggcctacgtc ctggcccgcg cgggccggcg cgcggaggcg agccggctcg tggccgagct
     1621 ggcggcgatg cccgcgggcg cggtctcgcc gctggcgatc gcggccgccg aaatcgcgct
     1681 cggaagaccc gaatcggcgc tcacggtcct ggagcgcgcc tgcgaccagc acgagagccg
     1741 gattctctgg atccgggtcg atcagcgctt cgatccgatc cggaaggacc cccgctacgc
     1801 gaacctgctg gaacgcatga tgtccgcctc agccacctga gatcaggggg ccgacaggcc
     1861 gcccagcatc gccttggcct cgtcgttctc cggatcgcgc cgcagggcct cgcgcgccag
     1921 ggcgatcgcg cgctcgtgca gggccgtgtc ggcctcgagc ccgctctccg cttcgagcag
     1981 gcgcgccagc atgagggtcc gctcgggcga gccgagcagg gacgccgccg tcgcggcgtc
     2041 gaggcggcgg atgagcgtgg tctgacccgc gagcagcacg ccgtaggcct gctcgaggac
     2101 ggcccgcgcc tcgtcgggcg agccctccag ccggagtccc gcggcgcgcg cgatcatggc
     2161 cgcgaccgtc tggatctgtc tcaggagata atcgtcggtc tgcttcatcg gttgcccgct
     2221 agacccggag cagcgtctcc gcgaacggcc actcctcgtc cacccattcc gccgcgacct
     2281 cgaatcccgc cgccttcgcc agcttcgcca cgtcgtcctt ctcgaacttg tacgagctct
     2341 cggtccagat gctctcgccg tccttgaagg cgacccgaag atcgagtgcc ggaatcgcga
     2401 cctcgtgggc gcgcttggcg accaggtgca tctcgatccg gcgctccgcc tcgcgccagc
     2461 gcgcttcgtg gcggaagagg gagagatcga acgaggcgcc caggtcgcgg ttcatgcggg
     2521 tcaggagatt tcgatcgaac gcggcggtca cgcccagcgg gtcgtcgtag gcgcggatca
     2581 gcgtgggcgc gggcttgacc cggtcggtgc ccaggagcag cgcgtcgccg ggcttcagcg
     2641 tgcggcgcac ctcgcgcagg aacgaccgcg ccgcgtcggg ctcgaagttc ccgatggtgc
     2701 tgcccaggaa gagcaccagg agatggtgcg cgctccggcg ctgcgccgcg acgttggcca
     2761 ggccggccag ataatcggcg tgaatcgcct cgaagcgcac ggcggggagc cgtcccagca
     2821 ccgtcgcgca cttggccagc gcggtctccg cgatgtcgat ggggtagtag tgggtcgtct
     2881 ggcgcgaggc gagcgcgtcc aggatccagc gcgtcttccg cccggtcccg ctccccagct
     2941 cggccacgag gagcggcgtg gcgagcccgc gcgtgatggc cggcgcgtgg cggcgcagga
     3001 tccgctcgtc cgcgcgcgtg agcccgtact cggggagcgc gctgatcgcc tcgaagagag
     3061 cgctcccaag ctcgtcgtag agaagggccg cgggcagctt cttctggccc gggcgggaga
     3121 gcccggcggt gatcgccgcg agcagctccg ggtccatgcc ggcgatcacg ccgggagcgg
     3181 ccttgccagc ggcctccgcg ccgccgcggc ccgcggccga tggctcggtc acccggccac
     3241 cgtccggaag gccccgtaca cgtaggggta cttcggctgg aaccagttcc ggaacgaacg
     3301 gcgcagcatg caggccgccg tccgcggcga gccccccttc atcacgtagt gcttgccgtc
     3361 gaagaaatcg gcggagtagc cgcgatagaa gggatgcgcc tcgaagccgg ggaagggctc
     3421 gaagagcgag gaggtccact cccagccgtt gccgaccagc tcgtccacgc cgaaggcgct
     3481 ggctccggcg gggtggcccc ccaccggcgt cgggtcccag cggtgaaaat cgaaattccc
     3541 gtgttcctcg cccggcggct ggttccccca tgggtattcg cgctcctcgc ccgagggcgt
     3601 tccgtaggcc gcgcgatgga actgcgcttc cgtcggaagc gagcggccgg cccagcgcgc
     3661 gaacgcggag gcttcggcgt ggctcacgta gaccggcgca tccatcgaga ggagcggctc
     3721 cgagaacatg ccgcggtggt gccaggcgcc cgctttccga gtccagaacg gcgggtggac
     3781 cagcttctgg cgctcccgcc attcccagtt ctcctcggtc caatggatcg gatcttcgta
     3841 gccgccgtcc ttgacgaact cgaggaagcg gccgttcgtc accttatagg cgtcgatctc
     3901 gaactccggc acctgcacga cgcgctctcc gaattcgttg tcccacccga acacgcccgc
     3961 actccgggag cggccgagcg tggccttgcc ggcggggacg cgcaccgtgc gcgacgcgtc
     4021 caggcccgtg gcggacggga tcggggcatc cggctttccc ttcttcagct tgagcgggag
     4081 ctgatgcagc agataggcca gcgtctcggc gtgcatcagc cggtgctcga tcgccatgtt
     4141 gatgatggtg cccgcgtcct ggccggcgcc gatcgggcgc ttgcccgtaa ggcactcgtc
     4201 cacggcctcc cggatccggc cgttgtaggc gcggatcggc tcgggacggg gccagacgtc
     4261 cgcggcgtcg ctcggcagct ggccgtcgac cggatcgatt ccgaacgcga acagcttgtc
     4321 gtagaccgga ttgaacgacg cgaggtcgta ggcctggcgg agcaggttgc cgtcgaacgc
     4381 ctcgagatgg cccaggtaga aggccagacg atgccgctcg gggatcgccc gctcgtacag
     4441 ggcctccggt ttcaggactt cgaagatctg gtcggtctgg gcacgcgcgc cggtcaggcg
     4501 ggcggcgagt gcttcagggg tttcgctcga tcggagcgtc tgggtcatgg tgcgcctcca
     4561 gggttgattc accctcgatt ttagacgaat cggcgccccg ccgcccccgt ttcgcgccgg
     4621 cccccgtggc cagccggagc cccttcggga ccgccatccg gtcggccagg tcgaaggcgc
     4681 gctctaccag cagcgccagg agggcggccg ggatggcccc ttccaggatc aggtcgttcc
     4741 ggcggagctg aatccccgag aggatcggct cgcccagccc tccggcgccg atcagggccg
     4801 ccagcgtggc cgatcccacg ttgatcacgg cgctggtccg gatccccgcc aggatcgccg
     4861 gggaggccag cggaaggcgg atccgggtca gctgcgcgcg ggccgacagg ggaaggcacg
     4921 ccgccgactc gatcaggggc gggggaatcg tggtgaggcc gacgtaggtg ttccggacga
     4981 tcgggagcag gctgtacagg aagagcgcga ccagcgccgg caggatcccg atcccgagca
     5041 ggggaatgag caccgcgagg agcgcgagcg aggggatggt ctgggcgagc ccggtcgcgg
     5101 ccaggaccgg cccgccgatc cagcgcgatc gcgccgcggc cacgccgagc ggcacgccga
     5161 tcagcaccgc ggcgagcagc gagagaagga cgagcttcag gtgctgtacg gtgttcttcg
     5221 cgatgcgggc ggctctcccc tcggacgcgg aagatgccgc cggcgctccg gggccggaga
     5281 tctccgcgag caaggactcg gcgaccgcgg ccgattcggc gccgcccagc accacgcgcg
     5341 cgttggcgcg gatcatccgc gcctgatcga tcgtcccggt cagacgccgc atcgccgcca
     5401 tcgcccgcgg ctcgcgccgg gccaggtcca gccggtagag ccacaccgcg tcgtagcggg
     5461 gaaagaaggc gcggtcatcc cgcagcacga ccagatgcat ccgctcgatc tgcgcgtcgg
     5521 tcgtgtagat gtccgtcgcg tcgatcttgc cgctcgccat ggcggcgtag gccagctcgt
     5581 gctggatgcc ggtcacgttc ttgcgcgaga ggccgtagcg cgcggcgagg ccggggaacc
     5641 cgtccgcgcg gcccaggaac tcgtgcgtga gacccaggcg gacctcgggg tgcccggcca
     5701 ggtcggacag cgcgcgcaga ccgaagcgcc gggccgcctc gggcgtcacc gcgatcgcgt
     5761 agctgtcgtc gaagccgatc ggctcgctca tgcccaggcc gatccgggcg agcgcggcgc
     5821 gcatctccgg cgggctgagc gggcgagggg cgtggagcac cacttcctgg acggtgccgg
     5881 tgtactcggg atagacgtcc acgttccccg agcggagcgc ctgaagcacg atctcggtgc
     5941 cgcccaggtt ccgccggtgc gccgcgtccg acactccggc ctcccgcatc gcggcggtca
     6001 gcgcatcgcc caggatccac gattcggcga acgccttgga ccccacggcg acgccgcgct
     6061 cggcacgggc cggaccgggt gcggcggcgc cggccagggc cgccgcgagg agccatgcga
     6121 ccccaggcct cacgtccatg ccaccgcccg ctgcgccgtg aggaacgccg tgacgaatgg
     6181 atcggcgggc cggtcgatca ggtcccggaa ggcaccccgc tgcacgaccc tccccgcgcg
     6241 catcagcgcg atctcgtcgc cgatcgtcgc cgcctcggcg acatcgtgcg tcacgaagag
     6301 gacggtcttg tggagccgct cgaagacggc gcgcaggtcg cgctggaggt cggcccggat
     6361 catcggatcg agcgccgcga gcggctcgtc gaggagcacg acctcgggat ccagcatcag
     6421 ggcgcgcatc agcgccacgc gctggcgctg gccgccgctc agctcggcgg gatagcgcgc
     6481 gagcagcgcg cgcggcatct gcgtgagctc gagaagctcg aggagccgct tttcccggcg
     6541 ctcgcgggac cagccgcggt cttcggcggc gatcgtgacg ttcgcgcccg ccgtcaggtg
     6601 ggggaagagg cccccttcct ggatgacgta gccgattcgg ggaaggaccg ccgggcgggt
     6661 ggagggtccc atgaccgctc cggccacgcg cacgacgccg cggtcgggga cgaggagccc
     6721 gacgaccagc cggagcagcg tgctcttgcc gcacccgctc ggtcctacga ccgccagcgt
     6781 ggtccgctcg cccacggcca ggtgaggcac gtcgagcgcc gtggcgtcgc cgaacttctt
     6841 gacgacgcct tccagctcga ccgggtcggc cggatcgggg cgatcggggg gatcgggcat
     6901 gcgggggcga tcctaccggt tcggcgctat actcggctac ccatgcggga ttccttccag
     6961 ccggcgcgcc tgctttctct cgtcggtccg acggtgctcc tcccggcgct ggtcgccgca
     7021 ttcctggcgg cgccggggct ttccttcgat tacttctggg acgatttctt cttcctcacc
     7081 aaggcacagg gaagcccgac cgcctacctt gtgcccaacg cggccgaggc gttctggcgg
     7141 ccgatccccc agggactcta cttccgtctc ctcttggcgc ttggcccggc cggtccgttc
     7201 gccggacact tcctgaacct gctcctgctc gccgcggccg ccatgctcct cgcctcgctc
     7261 gcgtcgcgtc tcgcggggcc gcgcgcgggc cttctggccg ggctggtgtt cgcggcgtcg
     7321 gccgcgctcc cctcgctggt ggcatggacc agcgcctgcc aggatctgat ggcgatggtc
     7381 ttcctgttga tcgcgttcca cctgagggag tcggggaaga tcgcggggtc ggccggcgtc
     7441 gcggccttgg cgctcctctc caaggagacc gccgcggtgg cgcttccggt gctcgtcgct
     7501 tgggactggc tgctcgggcg ggagccggcg cggcttgtac gcggtgctct cctcttcggc
     7561 atcgtgaccg ccgcctggtt cgcggtgcat cccggtctcc gggctctggt ccacgccggt
     7621 ctccagagcg gcacgacggg atacgtcggg ctggagcggc ccgaacggtg gtgggagtac
     7681 ctcttccgct acgtcgccac gctgttcaat ttcccggtga acgccgtctc gtggcccgcg
     7741 cggctgacgg tcgccgggat cgccgcggcg gtcgccctgc tcgtgaccgc tcctggcgcg
     7801 ctgcagcggc tgaagccggc cgacgcgacg tcatccgacg atcccggtcg gggtacggcg
     7861 ccccgcccgc gccccaccgc gcgccggctc cagatcgcgg gactcctcat cgcgctgccg
     7921 ccgatcctgc tcccgtccct tctcgtccgt ccctgggcca cctacttcgc cgcgctcgcg
     7981 gtgcccgggt tcgcgcttct cgcggccatg cgactcgccc ggctcacgcc gcgcacggcg
     8041 gcgatcgcga tcgccgcgtt tctcctcctc ggcgtgtggg cgcgcggcat ggacgcgcgg
     8101 gggaacggcc gcgtgctcac ggaacagcgc ttcgccgaag cctcgctcgc cacgcgccgg
     8161 gtccgggagc gcttccggaa gatcgtgccc gagatcccct ccggcgcgcg ggtgctcctc
     8221 tcggtggcgg gaaccggcac gctcggcatc caccagaccc tgcaggacgg gcaggcgctc
     8281 cgccattggt accgcgaccc caccatcgcc accgtgcagc cggagtggaa gcggccgccc
     8341 ggcgccgacg agttcctggt ccgggtcacc cccgatctcc aggtgatcca gatcgacccc
     8401 acgacgtcgc ggttccgcac ggagggagac gaccccgacc gcgaggagat cgccaagccg
     8461 atccgctcct acgcgcgcgg tctggccgcg tcgggcgcga ccgatcccgc cgtctggatc
     8521 ctggagcgga tggccaccgc cgattccacg cggcttcgct cgtacgatct ccgcctggcg
     8581 gcgctcgcgc ttcacgccgt gcatcgggac ggcgaggcgg ggcacctgct gcgtctcgcg
     8641 ccgccgctcc agcgctggga ggcgctcgac atggcgggga aggtgtacgc cgagccgacg
     8701 cacgatcccg cgcacgacgc gctggccttc tggcccttcg gcgtctcgcc cgacgatccg
     8761 ggcgccctcc gctacctgat cggccagtac cggatcggag gctattaccg gcaggcgcgc
     8821 gagctggccc gccgcctgga gcgtttggca cccggcgact ccctcgcgct ggccacgatc
     8881 cgcttcaacc cgctccggga atagccctcg tgctcctggc tgagctggca tccacctcgc
     8941 gcgaggtcgc cgcgaccggc gcccgctccg tgaaggcgga gcgcctcgcg gcgctgctcc
     9001 gccgcctgga gggcgacgag atcgagatcg gcgtcgcctg gctctcgggg catccgcggc
     9061 aatcgcgcct ggggatcggg ggcgccacgg tgcacggcgt gcgcggctcc gaggcggcgg
     9121 ccgcgccatc gctcaccctg gccgacgcgg acacggcgct cgcgcgcatc gccgcgctcg
     9181 cgggcgcggg ttcgggcggc gagcgccagc gatcgctcgc ggccctcttc gcccgcgcca
     9241 ccgccgagga gcaggacttc ctcgcccggc tcatcgtggg cgagctccgc cagggcgcgc
     9301 tcgccggcgt gatggaggac gcgctcgcca aggcgaccgg catcccggcg gcggagatcc
     9361 gccgcgccac gatgctctcc ggcgacgccg aggcggtcgc gcgcgccgtg ctccacgagg
     9421 ggcgcgcggc gctcgcccgg tttcaggtgg agctgttccg ccccgtgcag ccgatgctcg
     9481 cttctcccgc cgacgatctc gacgcggcgc tggagcggct gggcgaggcg gcgctggagt
     9541 acaagctgga cggcgcgcgc gtccagctcc acaagtcggg cgaggaggtg cgggtcttct
     9601 cgcgccggat gaacgacgtc acggtcgcgg ttcccgagct ggtcgaggcg gcccgcgcac
     9661 tgcccgcgcg ggagatgatc ctggacggcg aggcgatcgc gctgcgcgag gacggccgcc
     9721 cgcatccctt ccaggccacg atgcgccgct tcggccgccg gctggacgtg gaggccctgc
     9781 ggggagagct cccgctcacg ccgttcttct tcgatctcct gcgcctggac ggcgcggact
     9841 tcttcgacgt cccggcccgc gaccgcttcg aggcgctcgc gcgcgtcgct ccggcgctcg
     9901 tcgtaccgcg aagggtcacc ggcgacgtgg acgccgcgga ggcctttctc cgggaggcgc
     9961 tggcgcgcgg tcacgagggg ctcatggcca aggcgcccga agcgcgctat gaagcgggac
    10021 ggcgcggctt ttcgtggctc aaggtgaagg aggcgcacac cctggatctg gtggtgctcg
    10081 cggccgaatg gggacacggc cgccgcaagg ggttcctcag caacatccac ctgggggcgc
    10141 gcgatgccgc ctccggcggc ttcgtcatgc tggggaagac cttcaaggga atgaccgacg
    10201 cgatgctggc gtggcagacg cagcgcttcc aggagctggc ggtcgcgacc gacgggtaca
    10261 ccgtgcagct ccgccccgag ctggtcgtcg aggtcgcgtt caacgacgtg caggagagtc
    10321 cgcactatcc cgcggggctc gcgctccgct tcgcgcgcgt gaagcgctac cggcccgaca
    10381 aagcggccgc gcaggccgac acgctcgaaa ccgtgcgcgc catccacgag ggtcggttat
    10441 cctcgtagcg gggaggcaag cggagaaggg agttatcccc tggatgggat ttctcggact
    10501 cttctggatc gcgttccagg actggagacg cgaccgcgcc gaccgtctcg gcgccgcgct
    10561 cgcctactac agcatcctct ccatcccgcc gctgctcctt ctcctggccg gcatcgcggg
    10621 gctggtcttc gacccgcaat cggttcgggg tcggctgctc gccaccctcc aacagctctt
    10681 cggcgcccag ggcacttcgg cggttcggga catcctggcc gggatggacc ggaacggcca
    10741 cagcggcacc gccacgctcg tcggattcgg gaccctgctg ctcggagcct ccggcgtctt
    10801 cggtcatctc aaggactcgc tcgatacgat ctggaacgtg cggcaggaca aggggagcgg
    10861 ctggaagggc tggctcaagg acaacgtctt ctcgatcgtg gtgctgatgg gcgccgcgtt
    10921 cctgctcgtc atctcgctcg ccgtgaacgc cctgctcgcc gcggccagcg actacctgtc
    10981 gcggaatctc ccgggcggaa cgaccctttg gggcggggtc aacctgctcg tctccctcgc
    11041 cgtgctggca gcgctcttcg gcctgatgtt ccgctacatc ccggcggtga agctgcgctg
    11101 gcgcgacgcc gtcatcgggg ggctggtcac cgcggcgctc ttcatggcgg gcgaggcggc
    11161 gatcggcttc tacctgggaa agaccaacgt cggctccgcg ttcggcgtcg cggggtcgct
    11221 ggtggtgctc ctggtctggg tctactactc cgcgctcatc ttcttctacg gcgccgagtt
    11281 cacgcaggcc tacgccgagc ggcggggcac gcgccccgcg ccgcgccgcg agcgcacgcc
    11341 ctagacgggg ccccgccggc tcggaacccg ctcaagtccg tcgagcgccc ttccgatatt
    11401 cggtgggcca ccatgggcca cccgatcgcg atcctcttcc tgcacggcat cggccgcacc
    11461 gagccgggct actcgcgtcc tctcgccgag gccatcgccg cgcgcttcac gcggtccgcc
    11521 ggcggcgaga cctccaaccc cgaagcgcag atcgtcttcg aagaggtgaa ctggagcggc
    11581 gcgctccagc cggccgagga cgacctcggc gcgcgccttc gcgccgcggg accgctccat
    11641 ttcgggaggc tgcgcggatt catgctcgac ttcgcagccg acgcgatcgc ctaccagccg
    11701 acccacgccg accgctcggc gtacgacgcg atccacgcgg aggtcgcggc cagcctgggg
    11761 cgcctggcgc gccgcgcggg cgctcgcgcc ccgctcggcg tgatcggcca cagcctgggc
    11821 tcggtgatcg cgagcaatta cgtctacgat ctgacgaagc cgcgcgggag cttcatggct
    11881 cccgaagtgc gcgcccggat cgggaagacc ccgctcgagc gcggcgagac cctgtccctc
    11941 ttctacacga tggggagccc gatcgcccta tggagtctcc gctacccgga cttcggcgtt
    12001 ccggtgaagc tccccgtgaa gcgcctcgcc gggcatcacc ccgggctcga gccgcgctgg
    12061 gtcaacttct acgaccccga cgacgtgatc ggctaccccc tgaagccgct gaacgaggcc
    12121 taccgcgaag cggtcgacga ggaccgcgcg gtggacgtgg ggagctggct cacgcgatgg
    12181 aatccgctct cccataccgg atactggggc gatgccggcg tggccgacgt catcgccgcc
    12241 ggcgtgaccg aggcctggcg ccggagcagc gcgatcgctt tggtgggagc gaagaagcca
    12301 agaacccgcc ctcgacggac cgcctcccga acctgagtcg tctgcaaact tggccccctc
    12361 cttgcagcgg gcgtaggctt ccgcgcgtct gtcgcgcgcg ggacctatgg aggggacgac
    12421 gatgagacga ggacgcctgc ttctcgggct tctgatcgcc gcgatttccg tgatcagcta
    12481 ctactcgctc cagcagcaga acccggtcac gggcgagacg cagcacatcg cgatggacgt
    12541 ggaccaggaa gtggcgctgg ggctggaatc ggcgccgcag atggcccagg agatgggcgg
    12601 gctcgatccc gacgagagca tccagcagcg ggtgcagcgg gtcgggcagc gcgtcgtgca
    12661 gcagagcgag gcccgcggca cgccctacca gtatcacttc tacgcgctcg ccgatccgca
    12721 gacggtgaac gccttcgccc tccccggagg acctgtcttc atcacgcgcg ggctcctgtc
    12781 gcgcctggag aacgaagccg agctggccgg cgtgctgggc cacgaagtgg gccacgtcgt
    12841 gggccggcac acggcggccg cgatcgcgaa gagccagctg gcgcagggac tgatcggcgc
    12901 ggccggcgtc gcgggctccg acgatcaggg gggagggcaa cgctcggcgc agctcgccgc
    12961 gctcgtcgcc cagatggtgc agctgcgcta cggacggggc gccgagacgc agtccgacac
    13021 gctgggggtg cgcttcatga ccgaggcggg gtacgacccg cgcgccatgc tggaggtgat
    13081 gcggatcctg agcgaaagct cagggccggg tcgcgagccc gagttcatga gcacgcaccc
    13141 cgatcccgga aaccgcagcc aggtgatcca gggggcgatc gagcgacgct acccgaacgg
    13201 cattccgagc aacctgacgc tgggcgagcg gttcgaaact acctacgggt cgcgctgacc
    13261 cgcgcggccg cggccggagc cccgccgccc aggcgtagca cgttctgccg ggcgccggcc
    13321 gcgccgtcgt tgccggggct cggtccgccc gcgccggccg tgccgagcgt gtagctgttg
    13381 cccgactccg tgagcgtgct ggccacgtcg tagaggatcc cgatcgtcgg cccgccgccg
    13441 ccgccgccgc cgtggccccc gcgaccgccg gttccgccgg caccgcccgc cccgccgttc
    13501 ccgatcagcg aattgcagtc cttgcccgcg ctgccgccgg cgccgccggc tccaccacca
    13561 ccgccgggac cgccgctgcc gccgctgccg ccggctccgc cctggttcgt ccggagcacg
    13621 catctctcga cgacgaggtt ggtcgtcgcg acggcggcaa tcgcgaagga gcctccgccg
    13681 ccgccgcccg ccaaggcgcc cgcacccgga gtgccgcccc ctcctccgcc gccgccgccg
    13741 ttgccggctc cgtccgtcgg cgagccgctg gcgccgccac cgccaccgcc gccgctcccg
    13801 cccgttccgt cgctcccgcc gtcagcgccg gccgccggct ggtaccccga cgtgccgagg
    13861 cctccgaccg cgacgccacc aggcccgtcc gtcccgggcg agccgccgcc gccggtgccg
    13921 ccggccgtgc cgttgtgctt gcggccggtc acgagcccgg gatcaccgcc cgcgccgccg
    13981 ttcggcccgg agccggtgtc gccgggggcg gcgacgaaga ggctggcgag gtttccgccg
    14041 gctccgcccg cgccccccgc gtacgcgccg ctcccgccgc caccgcccgc gccc
//
LOCUS       DASSLZ010000314        10712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_625375, whole genome shotgun sequence.
ACCESSION   DASSLZ010000314 DASSLZ010000000
VERSION     DASSLZ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10712
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625375"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFM00_11850"
     CDS             <1..536
                     /locus_tag="VFM00_11850"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7498984.1"
                     /translation="FVSARTRLEENDTMRYALALAILIAVIPACGTQQSLDTTVQAAT
                     VRATIDSLWSQYAVAANQRDSLTFGTLFHDDAVLVFSGAPTISGKAAIQEFLLALYTP
                     VSVTRLRVVPDDLRVHGPLAVETGTFEEDYLEAGASKTEAGRFTLIAEPGPAKRWLIR
                     RLVMLADSVRAGVAGGA"
     gene            533..2440
                     /locus_tag="VFM00_11855"
     CDS             533..2440
                     /locus_tag="VFM00_11855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HET7498985.1"
                     /translation="MSATRAASAPPAFEEALRTFGFPSFRPGQLEALETLHRRRRLLL
                     VAPTGGGKSLIYQLPATMLGGTSLVISPLISLMHDQVDALEAKGIRATYLASTLDDGE
                     AMERLGQIRGGEYRIVYVAPERLRFPGFRDLVRAMGCPLIAIDEAHCISQWGHDFRPD
                     YLQIRGFLELHPEALVLACTATATPVVRDDILRQLGFPADTPQIVRGFARPNLALRAR
                     SVASAAEAARAVDDTLREALGNAEAPRGGAIVYCLTRKDAEEEAKRLREDGWRAGWYH
                     AGLSGDHRSQVQRRFMRGELSVVAATNAFGMGIDRPDVRAVIHRSPPDSIESYYQEVG
                     RAGRDGEDASGLLLVRGQDIPRRRHLLERSASESAAGEAIFEHKWSMFLDLIRWAEGG
                     SCRHDAILRYFGDEAETLHGCGRCDVCAEIARGGAAAGGAEESGRIVIAVLGAVALVH
                     ERLGPKATAKFLRGEEDDRLIRAGLVGRPGWGALAGETEAWLGQVIQRCVAAGWVDFT
                     PGEYSLLVLTPTGRAVLEGKRPASLILPPRGTEAAKPRSRSRKRDAAPRTGGETDLDE
                     SLFETLREWRRAESLAGGVPAFVVASDRALRDIAAARPRTMDELLACHGIGPQKAERY
                     GGAILAIVRRV"
     gene            complement(2401..2694)
                     /locus_tag="VFM00_11860"
     CDS             complement(2401..2694)
                     /locus_tag="VFM00_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498986.1"
                     /translation="MIADTGFGRGERVGPLPKIATEPDPERIAQGWALRFVVEGLRAE
                     SFVRLYESLGYEACADPLRPELLPEGCTDCRLAAALGFRAIYTRRTIARIAPP"
     gene            complement(2691..3530)
                     /locus_tag="VFM00_11865"
     CDS             complement(2691..3530)
                     /locus_tag="VFM00_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HET7498987.1"
                     /translation="MNGWAMRITAGIDVGSTYTKVLLLREDRTILGRAVEPTGFRLAE
                     VAEGSFERLLQRLGLERDRVGYVVATGYGRHQASFADVKVTDLTASARGARFLFAGTQ
                     TVLDVGGQTMKATRIDETGKVKTFRLNDKCAAGTGAFLEKTARYLGYATEEIGPLVAT
                     SKEYPSISGVCAVFAESEVINHLSQGVAPADIMHGAIVSLVGRSVQLMKRARMGPEYT
                     LIGGILRFETMARVARESLSGHVNVPDGDLPQFVTALGAAWLGQQRMAKIAKEGSVGA
                     RTP"
     gene            complement(3527..4345)
                     /locus_tag="VFM00_11870"
     CDS             complement(3527..4345)
                     /locus_tag="VFM00_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HET7498988.1"
                     /translation="MAYAAGVDVGSTQTKAVVVDEAGVIAGRALIGTGANVMRAAENA
                     FRGALADGGLREEEVEFVVGTGYGRYKVTFGNAQVTEISCHGRGAVHMFPATRTVVDM
                     GGQDTKAIKVSARGEISDFCMNDKCAAGTGRFLGAASTALDIPLEQLGPTALEGERPV
                     KISTTCTVFAESEVLSWLGKGKKIGDVLLGVHESIASRAVSLLWRVGVEEQVTFTGGV
                     ARNVAMIEALNRQLGLQVNVSEESHFMGALGAALFARDHIFTSREPARAGRARP"
     gene            complement(4349..5656)
                     /locus_tag="VFM00_11875"
     CDS             complement(4349..5656)
                     /locus_tag="VFM00_11875"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HET7498989.1"
                     /translation="MTPAEAAAIVGRGNRDGARLFREWFDGLGRAAAEGRGGAYVFVM
                     GSLGELLRVFDLPLVFPEINSLQTAVRHVAHEYLNEAEDMGYSSDLCGYVKADVAAQH
                     RGGELPMGRIPKPAIAVLTNACNTYVKWAEIWERMYRIPAYVLDVPGTRADGTQTWRG
                     DRDFENDRRYVAAQLRELIALLEQVTGKRFDVDRLRESLVHANRLGRGWKRVLELNRS
                     RPSLFNALSDGTIYLGVCNGFRGAPEGGDYFEQLVEEMELKRKEGIGTLVDERFRLVF
                     VGVPCYPIFRRFTELFTEHGGTFVTSTYLWFASGGANRAFEYDPRDPVESLAEGLLVS
                     VRDAMDSMFYPTTALAEMIDSYAVDGVVFHPIKSCRTVSTGLADNRRALMERRDVATL
                     FVESDMMDQRVVSEAQLKNRIDAFFEGLVSRRMAPAGGDGAGR"
     gene            complement(5653..6840)
                     /locus_tag="VFM00_11880"
     CDS             complement(5653..6840)
                     /locus_tag="VFM00_11880"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HET7498990.1"
                     /translation="MSAGPPVVFPEWKGRDLEGILAACQDLLEDVSFPTVRRWREAGG
                     RVVGHFQVYFPEEIAHAAGLLPFKVRGAPIEAAHADSRFGSYLCSIIKTSLELALART
                     VTMELFVTHPICDAARNLAAVWGRNFTYPCQILYLPQNAASPSAAGYLRGEYDRLRRT
                     IESVAGVAVTDEALRRSIAVFNENRLLLRGLYAIRRERPWLVAAHEAYALTAMGGLIP
                     REEHNEMLRRVVRELEQRPARAQDRIRVVFEGGFCEQPPLDLIRAIGRSCYVVDDDLL
                     IGLRWILEDVPAGDDPLADLAAAYLDRSSYSPVQHDPRKPKERMLLERVERSGARAVI
                     LAAAKMCEPGLEEQVAYVQALDAARIPYFVTEFEESMTSFEQIEIQIETFVENILFEE
                     EPA"
     gene            complement(6837..7595)
                     /locus_tag="VFM00_11885"
     CDS             complement(6837..7595)
                     /locus_tag="VFM00_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET7498991.1"
                     /translation="MTVRMEVDAGIATLTLDHPPLNVLTRAVLRELREASLRLRDEVG
                     VRAVVLAATGKHFSAGADVGEHLPPAFRDLIPEMVGTVEAIASLPVPVIAAVRGRCLG
                     GGFELVQAADIMVAGESASFGQPEILLGVTAPIACALLPRRGPYGHAAEILFTGDPFG
                     APRAAAAGFVQRLVPDDQVESEARAIAARCARHSAAALHATKRTLRAAVGRTRPEALQ
                     AAAAIYLEEVMDTRDALEGLRAFTEKRTPVWEHR"
     gene            complement(7611..8360)
                     /locus_tag="VFM00_11890"
     CDS             complement(7611..8360)
                     /locus_tag="VFM00_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008387082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7498992.1"
                     /translation="MDLGLQGRVAVVTGGASGIGAATALGLAREGCDVAVVDVRFGGA
                     ADHVLEGIRAAGRRALRVEGDVRDAAFGAQGIGRIVAEMERVDILVCAAGITADAVCW
                     KMTPAQWDDVIAVNLTGCFHWNRAAASVLRERGWGRIVNIASINGLRGKAGQANYAAS
                     KGGMVALSKSLAKELGRSGVTVNVVAPGMVKTPMTAPLAGRFVEAAIDESAVGRIAEP
                     EDCASAVAWLCSESARHVTGQVLKVDGGQYV"
     gene            complement(8374..9498)
                     /gene="oah"
                     /locus_tag="VFM00_11895"
     CDS             complement(8374..9498)
                     /gene="oah"
                     /locus_tag="VFM00_11895"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018807 -
                     6-hydroxycyclohex-1-ene-1-carboxyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HET7498993.1"
                     /translation="MLKNHTLFPEYRFQHIGYEMRPALDPCGRAAEGLFHAWIVLDNP
                     KQLNSYTTDMAREVGIAFRQASLDRRVVSVVFTATGDKSFCTGGNTEEYATYYAGNPQ
                     EYKQYMRVFNDMIDAILACDKPVVNRVNGMRIGGGQEIGMACDFSIAADTARFGQAGP
                     KHGSAPDGGSTDFLPVYVGFGRAIESAVLCETWSAHRAMFLGLVNEVVPVLRLDGRWI
                     PNPLVVTDRFLDEMGRIVYGDFRTGPDLALAKETFARAKTDLSLLDKAVESLCAKLLM
                     LMPDCTAKTIASLRKHKLHHWQANSGTNRDWLALNMMTEARAGFRAFHEGPKGRREVD
                     FIRLRQMLAEGHPWDDELYRAISPQCQPPVPPGVPEPAGS"
     gene            complement(9553..10596)
                     /gene="had"
                     /locus_tag="VFM00_11900"
     CDS             complement(9553..10596)
                     /gene="had"
                     /locus_tag="VFM00_11900"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HET7498994.1"
                     /translation="MKAIRLTASGAPLAVVDVPEPEPRAGEVVVRVAGCGVCHTDIGF
                     WRDGVPTRHPLPITLGHEVSGIVADAAPECRDWIGREVIVPSVIPCGACDLCRSGRGN
                     ACRSQIMPGNDLDGGFAERVAVPARGLCPVTERNGYSLADLSVVADAVTTPYQAVVRS
                     GLQSGDLAIVVGAGGVGGYAVQITAALGARVVVLDVDAAKLAGIAGHGASLALDPSAM
                     DFKSLKKEIADSAKAWGVPPYGWKIFECSGSAAGQETAFGLLGTAGVLMVVGFTLAKV
                     QLRLSNLMAFDATAQGTWGCRPELYPEALRMVTSGRVTLHPFIERHPLRDGPEVVRRV
                     ADHEIQKRAILEP"
BASE COUNT         1534 a         3886 c         3724 g         1568 t
ORIGIN      
        1 ggttcgtctc ggcccgaacc cgcctagagg agaacgatac gatgcgctac gccctggctc
       61 tcgcgatcct gatcgcggtc atccccgcgt gcggcacgca gcagtcgctc gacaccaccg
      121 tccaggccgc caccgtgcgc gccaccatcg actcgttgtg gtcccaatac gcggtcgccg
      181 cgaaccagcg ggacagcctc acgttcggca ccctgttcca cgacgacgcg gtgctggtct
      241 tctcgggagc gccgacgatc tcgggtaagg cggcgatcca ggaattcctc ctcgcgctct
      301 atacccccgt ttccgtgacc cggctccgcg tcgtgcccga cgacctccgc gtgcacggcc
      361 ccctcgccgt cgagacggga acgttcgaag aggattacct cgaggcggga gcgtccaaga
      421 ccgaggcggg gcggttcacg ctgatcgcgg agccggggcc cgcgaagcga tggctgatcc
      481 ggcgcctcgt gatgctcgcc gactcggtgc gggcgggagt ggcgggcggc gcttgagcgc
      541 gacgcgcgcg gcatccgctc ctcccgcctt cgaggaagcc ctccgtacct tcggcttccc
      601 gtcgttccgg ccgggccagc tcgaagccct cgagacgctg caccggcggc gccggctgct
      661 gctcgtcgcc cccaccgggg gcggcaagag cctcatctac cagctccccg cgacgatgct
      721 cggcgggacc tcgctcgtga tctccccgct gatctcgctg atgcacgacc aggtggacgc
      781 gctcgaagcg aagggaatcc gcgcgaccta tctcgcttcg acgctggatg acggcgaggc
      841 gatggagcgc ctcgggcaga tccggggcgg cgagtaccgg atcgtctatg tcgcgccgga
      901 acggcttcgg ttccccggat tccgcgatct cgtgcgcgcc atgggctgtc ccctgatcgc
      961 gatcgacgag gcgcactgca tcagccagtg ggggcacgat ttccggcccg actacctcca
     1021 gatccgcggc ttcctggagc tccatcccga agcgctcgtg ctggcctgca ccgccacggc
     1081 cacgccggtc gtgcgcgacg acatcctccg ccagctcggg ttccccgccg acacgccgca
     1141 gatcgtgcgc gggttcgcgc gcccgaacct ggccctgcgg gcccggagcg tcgcgagcgc
     1201 cgccgaggcg gcgcgggcgg tggacgacac gctgcgcgag gcgctcggga acgccgaggc
     1261 gccgcgcggc ggggccatcg tctactgcct cacgcggaag gatgccgagg aggaggcgaa
     1321 gcgcctccgc gaagacgggt ggagggccgg ctggtaccac gccggtctct cgggcgacca
     1381 ccgcagccag gtgcagcgcc gcttcatgcg cggcgagctg tcggtcgtcg cggccacgaa
     1441 cgctttcggc atggggatcg accggcccga cgtgcgcgcc gtgatccatc gctcgccgcc
     1501 cgattcgatc gagtcctact accaggaggt cggccgcgcc ggccgcgacg gcgaggacgc
     1561 ctcggggctc cttctcgtgc gcggccagga catcccgcgg cggcgccatc tcctcgagcg
     1621 gagcgcgtcg gagtcggcgg cgggcgaggc gatcttcgag cacaagtggt cgatgttcct
     1681 cgatctgatc cgctgggccg agggcggctc ctgccgccac gacgcgatcc tccgctactt
     1741 cggcgacgag gcggagacgc tgcacggctg cgggcggtgc gacgtctgcg ccgagatcgc
     1801 ccgtggcggc gcggccgccg gcggcgccga ggagagcggg cggatcgtga tcgccgtgct
     1861 cggcgcggtg gcgctcgtgc acgagcgcct gggccccaag gcgaccgcca agttcctgcg
     1921 cggcgaggag gacgaccggc tgatccgcgc cggcctcgtg ggccggccgg gctggggggc
     1981 gctcgccggc gagaccgaag cctggctggg acaggtgatc cagcgctgcg tcgccgccgg
     2041 atgggtggac ttcacaccgg gcgagtattc gctcctggtg ctcacgccca cggggcgggc
     2101 cgtgctcgag ggaaagcggc cggcctcgct gatcctgccg ccgcggggga cggaagccgc
     2161 gaagccccgc tcccgctccc gcaagcgcga cgcggcgccg cgaacgggcg gcgagaccga
     2221 cctcgacgag tcgctcttcg agacgctgcg ggagtggcgg cgggccgaat cgctcgcggg
     2281 cggggtgccc gcgttcgtcg tggccagcga tcgggcgctg cgggacatcg cggcggcgcg
     2341 cccccgcacg atggatgagc ttctcgcctg ccacggcatc gggccgcaga aggcggagcg
     2401 ttacggcggc gcgatcctcg cgatcgtgcg ccgcgtgtag atcgcgcgga acccgagcgc
     2461 ggccgccagg cggcagtcgg tgcatccctc gggcaagagc tcgggccgga gcggatcggc
     2521 gcaggcctcg tagcccagcg actcgtagag ccgcacgaac gattccgcgc gaagcccctc
     2581 gaccacgaag cgcagcgccc atccctgggc gatccgctcg ggatcgggct cggtggcgat
     2641 cttcggcagt gggccgaccc gctccccgcg cccgaatccg gtgtcggcga tcatggggtt
     2701 ctcgcgccca cggaaccttc cttcgcgatc ttcgccatcc gctgctggcc gagccaggcg
     2761 gcgccgagcg ccgtcacgaa ctgcggcaga tcgccgtccg ggacgttgac gtggccggac
     2821 aacgactcgc gcgccacgcg cgccatggtc tcgaagcgca ggatgccccc gatcagcgtg
     2881 tactcgggcc ccatccgcgc ccgcttcatg agctggaccg agcggcccac cagcgagacg
     2941 atggcgccgt gcatgatatc ggcgggagcg acgccctggg acagatggtt gatcacttcg
     3001 gactcggcga agaccgcgca gaccccggag atggatgggt attccttgga ggtggcgacc
     3061 agcgggccga tctcctcggt cgcgtacccg agatagcgcg cggtcttctc gaggaaggcg
     3121 cccgttcccg ccgcgcactt gtcgttcagg cggaaggtct tcaccttgcc cgtctcgtcg
     3181 atgcgggtcg ccttcatcgt ctgccctccc acgtcgagga cggtctgggt gcccgcgaag
     3241 aggaatcgcg cgccgcgcgc ggaggcggtg aggtcggtca ccttgacatc ggcgaacgac
     3301 gcctggtgcc ggccgtaccc ggtcgccacg acgtatccca cgcggtcgcg ctccaggccc
     3361 aggcgctgca ggagccgctc gaaggagccc tcggcgacct cggccagccg gaacccggtc
     3421 ggctcgaccg cgcgcccgag gatcgtccga tcctcgcgga gcaggagcac cttggtgtag
     3481 gtggacccca cgtcgattcc ggccgtgatg cgcatcgccc agccgttcat ggccgcgccc
     3541 gcccggcccg ggccggctcg cggctcgtga agatgtggtc gcgcgcgaac agggcggcgc
     3601 cgagcgcgcc catgaagtgg gattcctcgc tcacgttgac ctgaagtccg agctggcggt
     3661 tcaaggcctc gatcatcgcc acgttccgcg ccacccctcc cgtgaaggtg acctgctcct
     3721 cgacgccgac gcgccagagg aggctcacgg cgcgcgaggc gatcgactcg tgcacgccga
     3781 gcaggacgtc cccgatcttc ttccccttgc ccagccagga gagcacctcc gactccgcga
     3841 acaccgtgca ggtggtgctg atcttgaccg gccgctcccc ctcgagcgcg gtcgggccga
     3901 gctgctcgag cgggatatcg agggcggtcg acgctgctcc gaggaagcgt cccgttcccg
     3961 cggcacactt gtcgttcatg cagaagtcgg agatctcccc gcgcgcggac accttgatcg
     4021 ccttcgtgtc ctgtcccccc atatccacga cggtgcgggt ggcggggaac atgtgcaccg
     4081 cgccccgccc gtggcagctg atctcggtca cctgggcgtt gccgaaggtc actttgtacc
     4141 ggccgtagcc ggtgccgacc acgaactcga cttcctcctc ccgcaggccg ccgtcggcca
     4201 gggcgccccg gaacgcgttc tcggccgcgc gcatcacgtt ggcaccggtc ccgatcaggg
     4261 cgcgcccggc gatgacgccc gcctcgtcca ccacgaccgc cttggtctgg gtcgagccga
     4321 cgtcgacccc cgccgcgtac gccatcggtt acctccccgc tccgtcgccg ccggccggag
     4381 ccatgcggcg cgacaccagc ccctcgaaga acgcgtcgat ccggttcttc agctgcgcct
     4441 ccgagaccac gcgctgatcc atcatgtccg actcgacgaa gagggtcgcg acgtcgcggc
     4501 gctccatgag ggcgcggcgg ttgtccgcga ggccggtcga gacggtgcgg cagctcttga
     4561 tcggatggaa cacgacgccg tccaccgcgt aggagtcgat catctcggcc agcgcggtcg
     4621 tcggatagaa catgctgtcc atcgcgtcgc gcacgctcac gaggagccct tcggccaggc
     4681 tctccaccgg atcacggggg tcgtactcga acgcgcggtt cgcgccgccc gaggcgaacc
     4741 agagataggt ggaggtgacg aacgtcccgc cgtgctcggt gaagagctcg gtgaagcgcc
     4801 ggaagatcgg gtagcagggc acgccgacga agacgagccg gaagcgctcg tccacgaggg
     4861 ttccgatccc ttccttgcgt ttgagctcca tctcctcgac cagctgctcg aagtagtcgc
     4921 ccccctcggg cgcgccccgg aagccgttgc acacgccgag gtagatcgtg ccgtcggaga
     4981 gggcgttgaa cagcgacggg cggctccggt tcagctccag gacgcgcttc cacccgcggc
     5041 cgaggcggtt ggcgtggacc agcgactcgc gcaggcggtc cacgtcgaac cgctttcccg
     5101 tcacctgttc caggagcgcg atcagctccc gaagctgggc cgcgacgtag cgccggtcgt
     5161 tctcgaagtc gcggtcgccg cgccaggtct gcgtcccgtc ggcgcgcgtc ccgggcacgt
     5221 cgagcacgta agcgggaatg cggtacatcc gctcccagat ctcggcccat ttcacgtagg
     5281 tgttgcacgc gttcgtgagc accgcgatcg cgggcttggg gatccgcccc atcgggagct
     5341 ccccgccgcg gtgctgcgcg gccacgtccg ctttcacgta gccgcagagg tcggaggagt
     5401 agcccatgtc ctcggcttcg ttcaggtact cgtgcgccac gtggcggacg gcggtctgca
     5461 gggagttgat ctccggaaag acgagcggca ggtcgaagac ccgcaagagc tcccccaggc
     5521 tacccatgac gaacacgtag gcgccgccgc ggccttcggc ggcggcccgg cccagcccgt
     5581 cgaaccattc gcgaaagagc ctcgccccat cgcggtttcc cctgccgacg atcgcggccg
     5641 cttcggcggg cgtcatgccg gctcctcttc gaagaggatg ttctcgacga acgtctcgat
     5701 ctggatctcg atctgctcga acgaagtcat gctctcctcg aattccgtga cgaagtaggg
     5761 gatgcgcgcg gcgtccagcg cctggacgta ggccacctgc tcttccagcc ccggctcgca
     5821 catcttggcc gccgccagga tcacggcccg ggcgccggag cgctccacgc gctcgaggag
     5881 catccgctcc ttgggctttc gcgggtcgtg ctgcaccggg ctgtagctgg agcggtccag
     5941 ataggccgcc gcgagatcgg cgaggggatc gtcgccggcg ggcacgtcct ccaggatcca
     6001 gcggaggccg atcaggagat cgtcgtccac cacgtagcag gagcgcccga tcgcgcggat
     6061 caagtccagc ggtggctgct cgcagaagcc tccctcgaag acgacgcgga tccggtcctg
     6121 cgcgcgcgcc gggcgctgct ccagctcgcg cacgaccctg cgcagcatct cgttgtgctc
     6181 ctcgcgggga atcaggccgc ccatggctgt gagcgcgtac gcttcgtgcg ccgcgaccag
     6241 ccaggggcgc tcgcgccgga tcgcgtacag gccgcggagc agcagccggt tctcgttgaa
     6301 gacggcgatg gagcggcgga gcgcctcatc cgtcaccgcg acaccggcga ccgactcgat
     6361 cgtgcggcgg aggcggtcgt actcgccgcg gaggtacccg gcggcgctgg gggaggcggc
     6421 gttctgcggc aggtagagga tctggcaggg gtacgtgaaa tttcggcccc acaccgccgc
     6481 cagattccgc gccgcgtcgc agatcggatg ggtcacgaac agctccatcg tgacggtcct
     6541 ggccagcgcc agctcgagcg acgtcttgat gatcgagcag aggtaggagc cgaagcgtga
     6601 atcggcatgc gccgcctcga tcggagcgcc gcgcaccttg aacgggagga gccccgccgc
     6661 gtgcgcgatc tcctccggga agtagacctg gaaatgcccg acgacccggc cgccggcctc
     6721 gcgccagcgg cgcacggtcg gaaacgagac gtcttcgagc aggtcctggc aggccgcgag
     6781 gatcccctcg aggtcgcgcc ccttccattc cgggaagacc accggcggtc cggcgctcat
     6841 cggtgctccc acacgggggt ccgcttctcg gtgaaagcgc ggagcccctc cagcgcgtcg
     6901 cgggtgtcca tgacctcttc cagatagatc gccgcggcgg cctggagcgc ctccgggcgc
     6961 gtgcgcccca ccgccgcccg cagcgtgcgc ttggtcgcgt ggagcgccgc cgcgctgtgg
     7021 cgcgcgcatc gcgcggcgat cgcgcgcgcc tcggactcca cctggtcgtc gggaacgagg
     7081 cgctgcacga atcccgccgc ggcggcgcgc ggcgcgccga acgggtcgcc ggtgaacagg
     7141 atctccgccg cgtggccgta ggggccgcga cgcggaagaa gggcgcaggc gattggcgcc
     7201 gtgacgccga gcaggatctc gggctggccg aacgaggcgc tctcgccggc caccatgatg
     7261 tccgccgcct ggaccagctc gaagccgccc ccgaggcagc ggccgcgcac ggccgcgatc
     7321 acgggcacgg gaagcgaagc gatcgcctcg acagtgccca ccatctcggg gatcaggtcc
     7381 cggaacgccg gcggcaggtg ctctccaacg tcggcgcccg cggagaagtg cttgccggtc
     7441 gcggcgagga cgacggcgcg gacgcccacc tcgtcgcgga gccggagcga ggcctcgcgc
     7501 agctcgcgga ggaccgcgcg cgtcagcacg ttgagcggcg ggtgatcgag cgtgagggtg
     7561 gcgattcccg cgtccacttc catccgaacc gtcatgcgac cggcctcctt ctagacgtac
     7621 tgcccgccgt ccaccttgag aacctgcccc gtcacgtgcc gtgcgctctc ggagcagagc
     7681 cacgccacgg ccgacgcgca gtcctcgggc tcggcgatcc tcccgaccgc gctctcatcg
     7741 attgccgcct cgacgaagcg gccggcgagc ggagcggtca tcggggtctt caccatgccc
     7801 ggcgcgacga cgttcaccgt cacgcccgac cggccgagct ccttcgccag cgacttcgag
     7861 agcgccacca ttccgccctt ggacgcggca tagttcgcct gccccgcctt gccgcgcaga
     7921 ccgttgatcg aggcgatgtt cacgatgcgc ccccagccgc gctcgcggag cacggaggcc
     7981 gcggcgcgat tccagtggaa gcaccccgtc aggttcaccg cgatcacgtc gtcccactgc
     8041 gccggcgtca tcttccagca caccgcgtcg gccgtgatcc cggccgcgca gaccaggatg
     8101 tcgacgcgct ccatctccgc gacgatcctg ccgatcccct gggcgccgaa tgcggcgtcc
     8161 cgaacgtctc cctcgacgcg cagcgcgcgc cggcccgccg cccggattcc ttcgaggacg
     8221 tgatccgccg cgcccccgaa ccggacgtcc accaccgcca cgtcacaccc ctcgcgggcc
     8281 agcccgagcg cggtcgcggc tccaatcccc gaagcgccgc ccgtcacgac ggcgacgcga
     8341 ccctgcagtc cgagatccat cgcgcgccgg cgctcagctc cccgccggct ccggcacgcc
     8401 cggcggaacg ggcggctggc actgcggcga gatggcgcga tagagctcgt cgtcccacgg
     8461 gtgcccctcg gcgagcatct ggcggagccg gatgaaatcg acctcgcgcc ggcccttggg
     8521 cccttcgtgg aaggcgcgga agccggcgcg cgcctccgtc atcatgttga gcgcgagcca
     8581 gtcgcggttc gtgccgctgt tcgcctgcca gtggtggagc ttgtgcttgc ggaggctcgc
     8641 gatcgtcttc gccgtgcagt cgggcatgag catcaggagc ttcgcgcaga gcgactcgac
     8701 ggccttgtcg agaagcgaga gatcggtctt ggcccgggcg aaggtctcct tggccagggc
     8761 caggtcgggc ccggtgcgga agtcgccgta gacgatccgc cccatctcgt ccaggaagcg
     8821 gtcggtgacg acgagcgggt tcgggatcca tcgcccgtcc aggcgcagga ccggcaccac
     8881 ctcgttcacc aggccgagga acatcgcccg gtgcgcgctc caggtctcgc agagcaccgc
     8941 gctctcgatc gcgcggccga agccgacgta gacgggaagg aagtcggtcg acccgccgtc
     9001 gggggccgag ccgtgcttgg ggcccgcctg tccgaagcgg gcggtgtccg cggcgatgct
     9061 gaagtcgcac gccataccga tctcctggcc gccgccgatc cgcatcccgt tcacgcggtt
     9121 cacgaccggc ttgtcgcacg cgaggatcgc gtcgatcatg tcgttgaaga cccgcatgta
     9181 ctgcttgtac tcctgcggat tccccgcgta gtacgtcgcg tactcctcgg tgttgccgcc
     9241 cgtgcagaac gacttgtccc ccgtcgccgt gaagacgacc gagaccacgc gccggtccag
     9301 gctggcctgc cggaacgcga tcccgacctc gcgcgccatg tccgtggtgt agctgttcag
     9361 ctgcttcgga ttgtccagga cgatccacgc gtggaagagg ccctcggcgg cgcgcccgca
     9421 cggatcgagg gcgggacgca tctcgtagcc gatgtgctgg aatcggtact ccggaaacag
     9481 cgtgtggttc ttcagcatcg tttctctccc gcgcgccgcg cgcgctcgtg ccggggcgtc
     9541 cgccccgcga tcctagggct cgaggatggc ccgcttctgg atctcgtgat cggccacgcg
     9601 gcgcacgacc tcggggccgt cgcgcagcgg atgccgctcg atgaacggat ggagcgtgac
     9661 gcgccccgac gtgaccatgc gaagcgcctc ggggtagagc tcggggcggc agccccaggt
     9721 gccctgcgcc gtcgcgtcga aggccatcag gttgctcagg cggagctgga ccttggcgag
     9781 cgtgaacccc acgaccatca ggactcccgc cgtccccaag agcccgaacg ccgtctcctg
     9841 gcccgccgcc gagccggagc attcgaagat cttccagccg taggggggca cgccccatgc
     9901 cttggcgctg tccgcgatct ccttcttcag cgacttgaaa tccatggccg agggatcgag
     9961 ggccaggctg gcgccgtgcc ccgcgatccc ggcgagcttg gccgcgtcca cgtccagcac
    10021 gacgacgcgg gcgccgaggg cggccgtgat ctgcaccgcg tagccgccca cgccgccggc
    10081 gcccacgacg atcgcgagat cgccggattg aagcccgctg cgcacgaccg cctggtaggg
    10141 cgtcgtcacg gcgtcggcca ccaccgagag gtcggccagc gagtagccgt tccgctccgt
    10201 gacggggcag agcccgcgcg ccggaacggc gacccgctcg gcgaaccccc cgtcgaggtc
    10261 gttcccgggc atgatctggc tcctgcaggc gttcccgcgt cccgaacggc acaggtcgca
    10321 cgccccgcac gggatgacgg aggggacgat gacctcgcgt ccgatccagt cacggcattc
    10381 cggcgcggcg tcggcgacga tcccgctcac ctcgtgcccc aacgtgatcg gaagcggatg
    10441 acgggtcggg acgccgtcgc gccagaaacc gatgtcggtg tggcagacgc cgcacccggc
    10501 gacgcgcacg accacttcgc ccgcgcgcgg ctccggttcg ggaacgtcca cgacggcgag
    10561 cggcgcgccg gaggcggtga ggcggatcgc tttcatggct tcctccgggg gcggtttgcc
    10621 gaggcgggtg tcttcttgtt gtcgaggccg gccaggaaca ggtcggtgaa gcggtcgccc
    10681 agctccgcgg cgtgcagcga gccctcgggc cg
//
LOCUS       DASSLZ010000315        10309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_868651, whole genome shotgun sequence.
ACCESSION   DASSLZ010000315 DASSLZ010000000
VERSION     DASSLZ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10309
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868651"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(661..1239)
                     /locus_tag="VFM00_11905"
                     /pseudo
     CDS             complement(661..1239)
                     /locus_tag="VFM00_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
     gene            complement(1484..1897)
                     /locus_tag="VFM00_11910"
     CDS             complement(1484..1897)
                     /locus_tag="VFM00_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498995.1"
                     /translation="MASQKLVRCELRKSGEDAGSVRYVPLEIYGLWEFLMRNKHRFEV
                     ARSVASLWIDVDENPEVAYGENQYERVTEVTLLRYSESDGMFSKVNRYFPTQECSELK
                     NLLLRHYETVHEPGAPSLQFRERQGIWIRREARGR"
     gene            2012..2212
                     /locus_tag="VFM00_11915"
     CDS             2012..2212
                     /locus_tag="VFM00_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7498996.1"
                     /translation="MKRTVKSTAPWFLARLTPEVYCRRENRMVASCPEAQRAEACPCL
                     HGVETADALFEPNAGGENDPTP"
     gene            2392..3348
                     /locus_tag="VFM00_11920"
     CDS             2392..3348
                     /locus_tag="VFM00_11920"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7498997.1"
                     /translation="MTAPDDASRETPAFARLLLRVALGLAGRIPSSLAHAAAAAAATL
                     EYRLNPARREAVHANLREIARAGHPLLRHPADRERCARSVFRAYHRHLAEFLAQGRLT
                     PGAWDARFRFEGTEHLYRAAAAGRGAVIAGSHLGNWELAGLALARLGFRVHVVTGIQW
                     NPLLHGAVRELKERSGIEVSTPEDGFTPLIATLRQGGLVVLLVDGDVYTRGLTAPFFG
                     RAVSFPVGPALLARRAGAPLLHAHAAREGGGHRVVFDGADLPDRALSLGRDLARLTAR
                     VAAAQERNIAAQVGEWCIFRPFFGDAGAPAVAGDAGAPADAA"
     gene            3338..4510
                     /locus_tag="VFM00_11925"
     CDS             3338..4510
                     /locus_tag="VFM00_11925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7498998.1"
                     /translation="MPLRIAIVTQAYHPTVGGVTQHVEGTAASLRARGHQVTIVTSRH
                     PGTNGAEPGVFRLGRNFTLLYNGADNNITLGLGLEGALREHFERGAYDVVHVHCPLSP
                     SLPMLAIRSARQPVVGTFHSVSDSHAAFRIFRPILLPYFERLAHVIAVSEAARDDVLR
                     TFTRPISVVPNGVDLARFRPGVVPVARYRDGRVNILFVGRFDPRKGLPELLEACERLA
                     ERGTDFRLILVGDGRLRPGLERRARRSLPGRVVFEGQVPHERLPQYYATADLFCSPAR
                     GAESFGLVLLEAMALGVPVVASDIPGYRCVVSHGTEGLLTPPRNPAALADALGGLIED
                     PERRARMGAAGVRTAARFGWDRVARDLERIYASVLGARGEAPERAAAEAPETPAAV"
     gene            4510..5781
                     /locus_tag="VFM00_11930"
     CDS             4510..5781
                     /locus_tag="VFM00_11930"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7498999.1"
                     /translation="MGGAASLRLFRDTSFRALWIGQVVSVFGDRFTYLALLAVVIEHA
                     RTPANPAAELSWIPVVSFLPTILFGPWIGALVDTWDRKRVLVLSDAARGVVALLFLVV
                     VPHAGLTGAFGLVFALYVANSFFLPARSAILPEMVPEDRLVEANSLVTLAGVGATIAG
                     SLAGGVAIARFGWRVGFALDALTYFVSVVALLWIRVVPRVVVPSAPHPPHTAGDAYRA
                     LYADVREGARITLQSPRALGAIAATGLLWVAGGALHVAAPILLASRGHGIVAGVGTLL
                     AATATGMVAGSLALAARGGGGSPWGRTAAGLAGSGAALLLFARSHRTGADLALGFGAG
                     AFVALLLVTTEAALQGAIGPVARARVFALRDFTARLLVLASAGLCGVLLGRGMVRPEW
                     AVAGAGALLIAGALTLAFTARARDRGKGVPA"
     gene            complement(5807..6748)
                     /locus_tag="VFM00_11935"
     CDS             complement(5807..6748)
                     /locus_tag="VFM00_11935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET7499000.1"
                     /translation="MTIECTACHARYTIADSLVPPQGARVRCRKCSAVFLVQPSAPVV
                     TPEPLPVVPPTPEAPAHAATPPAPVEAPSQAHPPAAEPKAAPATPEPSRRSAAPETPS
                     QPADSGFDRFPSHAPPSHPAASEFERFPAASTSVAPAPHAAAPAQALFGTPESAATEP
                     AAGAAVFAPIPREETHVAPAPPPAQREPVHHEPAAPAHHEPAAPAHHEPAAHHETHRP
                     RISATTLPEGLSPEELQRHERARRLARVLASDIAIYNREKRERGIRDGNLVAVLGYEI
                     KKSWETYKERVGVDFANSTPYFRDALNEIVAEGKKIF"
     gene            complement(6835..9159)
                     /locus_tag="VFM00_11940"
     CDS             complement(6835..9159)
                     /locus_tag="VFM00_11940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET7499001.1"
                     /translation="MGRARAALAPERMAEQLVPGCGDPREPLATGCGPARVGAKRLPR
                     PCYPRRMHSLSDPVRYLKGVGPAMAGRLARLGILTVGDVLFHVPTGHRDRRDFTSIAD
                     LQPGVEATIVATVVSKRVDRRRGGRADLSASLRDESGFTRAVWFNQAFRGPQLVEGER
                     YVFSGKGQPFRGVELHNPEFEPYENESERVHTSRLVPRYGLTEGITERWLRARVRDAL
                     DELAPVPDLVLDDSSAGTTGGSAVTRSSPVPPDAAESSVTFSRARDHAALAGLPRLQD
                     AFERLHFPESLDQVEPARRRLALEELLRLQIALQLARVEHRANRRARPLGEGADLMRR
                     FLASLPFALTRSQREAIAAIEQDLDQEAAMRRLLLGDVGSGKTVVALAGAARAAGAGV
                     QTALLAPTAILAEQHAKTAERLLAGSGIRHALLTASTPQSERDRIRAELDSGALSLVI
                     GTHALLEKEVAFPALGFVVVDEQHRFGVRQRIALAEKGVGASGAHLLVLSATPIPRTL
                     AMAVYGDLDLSPLTEKPPGRTPVETTIVSGEDRRGLLDRIVREVEEGGRAFVVYPLVE
                     ESEAVDLKDAVSKAAALARVPALAAAGVALVHGRLKPDERRAAIERFREGEARVLVAT
                     TVVEVGLDIPDATLVVIEHPERFGLAQLHQLRGRVGRADRPGRCVLALSKDAGALARK
                     RVGVFQRVTDGFRLAEEDLRLRGPGELLGTAQHGFPELRVADPVGDSDLMEAARDLGA
                     ELLERGERERGREERLREWVEAHVAGAGHLLAGG"
     gene            9284..9871
                     /locus_tag="VFM00_11945"
     CDS             9284..9871
                     /locus_tag="VFM00_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499002.1"
                     /translation="MIRDAAGIVRAAAQAAQRRDQIVRFLPTGFPGRRFPFSGFSFSG
                     FSFGGLSFGSLSFGDLSFGSLSFCDPWFRHLSLDPGDPRGMALQGGIQARDPRLLALP
                     CHEPGSERVLGCVLIVGAAPEAQAINGGNAAAREDVDMVELEEAAGRAPMSVRDELGL
                     SQTSNARPQRAAPNCGPPRAPETRDPGAKPRPRPI"
     gene            9949..10173
                     /gene="rpmB"
                     /locus_tag="VFM00_11950"
     CDS             9949..10173
                     /gene="rpmB"
                     /locus_tag="VFM00_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET7499003.1"
                     /translation="MAKICDICGKGVQFGQNVSHANNVTKRRWEVNLQTVRATVEGRP
                     KRLRVCTRCIRTGRVQKSMPQAQALRARSA"
BASE COUNT         1374 a         3743 c         3634 g         1558 t
ORIGIN      
        1 gcgttcccgt caacgaaaaa ggcccgcgga gcgggcctga atgcgatttt tcgtcgcgcg
       61 gcgcgcgcgc gatcagacgg gatcgagatc gcgcatgtgc gtgtgccgcg tcttcaccgc
      121 tttcggcgca ccgtcgacca cgtcgatcgt gaactcgaac gtgacgcgca gacggcccgt
      181 gcgcgggagc gagcccagat cgatcggaac gacgatcgac ttccctccgg ccgacgtggt
      241 cgcggtggac cggtcggcct cgtacgtcga ctcccgctcg atctctccct cgctcaggat
      301 gccgtcctcg tcgcgcgagc ggacgatgcc gtcgtccttg gcgcggacga tctccggctc
      361 ctcgggctcg accacgagat cctgatcgcc gagcaccatc tcctcgcgcg gcgccggaat
      421 cgatgccggg ggagcttcca cgaacgggtt ccgctcgagg tacatcgggg ggggcatttc
      481 ctcgacgagc gcgtcctcgg cgagcatcgg cgcctcctcg accggctcgg gctccggctt
      541 gggcttcccg ccgcgcttca aggcctgcat gatgccgggc ttggcggggc gctcctcggc
      601 ggcgggctcc gccttcgagc cgcgcggcgc gcccagtccc ttgtccttct ccacgacgtc
      661 cttggagatc ttggagagga gcagcttcga gacgccgcgc agggtctcga agacgccggt
      721 cccgttcgcc gcctgcgcct cgtaggtcgg gacgttccag cggttcagct ccttgttcat
      781 ctggtcggtg ggaagcgcgt ccgggagatc gcgcttgttg aactggatca cccaggggac
      841 ggtcttgaga tcgaggccgt aggacttcaa gttctgctcg aggttcgtca ggctctcccg
      901 gttctccgcc atcttgtcgg ggctggagtc ggcgacgaag acgaccgcgt ccacaccctt
      961 aagcacgagc ttccgggtgg cgttgtagta gacctgcccc ggcaccgtgt acaggaggaa
     1021 gcgcgtcttc atgcccgaca gctcgcccag atccagcggg agcaggtcga agaagagcgt
     1081 ccggtcggtc tgcgtcttca tggagaccat gcggccgcga ttggactcgg ggacgctgtc
     1141 gtagatcttt tcgagattgg tcgtcttgcc gctcaaaccc gggccgtagt agacgatctt
     1201 ggcgttgatc tcccgtcccg aatagctgac cacgaccatg gctgggctcc cggattgaaa
     1261 tgtacgtttc gggtagcgcg gtcgcctgtc ctggcgccgg ccgcaggcct ggggagcacg
     1321 agacgtgcca ggatgggatc gggaggcgat ggcgcaacgg agccgcggcg ctgcaagtga
     1381 ttgggcgacc gtgggatcgc cggacggggc cggaaacggc cgggagagcg tgcgcgcggg
     1441 gtggagcgtc gtgcggatcg cacgatccca agggattaca ggctcagcgc cccctggcct
     1501 cgcgccggat ccagatcccc tggcgctccc ggaactggag gctgggcgcg ccgggctcgt
     1561 ggacggtttc gtagtgccgg agcagcagat tttttagctc cgaacattcc tgggtcggaa
     1621 aatagcggtt caccttgctg aacatgccgt cgctctccga gtagcggagc agcgtcacct
     1681 cagtgacccg ctcgtactga ttctccccgt aggccacctc ggggttctcg tccacgtcga
     1741 tccagagcga cgccacgctc cgggcgacct cgaaccggtg cttgttccgc atcaggaact
     1801 cccagagacc gtagatctcc agagggacgt accggacgct cccggcgtct tcacccgatt
     1861 tcctcagctc gcagcgaacg agtttctggc tggccatccc ctcacccttg cccgtggaag
     1921 gcgccgatgg cacacgtcat gcaacctccg gaaccggagc cggcatcgca ccggatcaga
     1981 ggctcgagat cgaacgagcc cggaggaccg catgaaacgc accgtgaaat ccaccgcccc
     2041 gtggttcctc gcgcggctga cccccgaagt ctattgccgc cgcgaaaatc gcatggtcgc
     2101 atcgtgtccc gaagcgcagc gcgccgaggc ctgtccgtgc ctgcacggcg tggaaaccgc
     2161 cgacgcgctc ttcgagccga atgcgggcgg cgaaaacgac ccgacgccct gactgcgcga
     2221 tcgtccacat tgtgctgagc ggggagcccc gccactctag tcagggcacc ccggcgagtc
     2281 aaatgtttca agccccgaag cgcggcaccg cctgcgcgat accggagagc aggcttcaac
     2341 ccgccgccgg cgcgccttga cacaccggat tcgcctccta tagttgccgc ggtgacggcc
     2401 cccgacgacg cttcacgaga aacgccggcg ttcgcccgcc tcctccttcg cgtcgcactc
     2461 ggcctggccg gccggattcc ctcctcgctc gcccatgccg cggccgccgc cgccgccacg
     2521 ctcgagtacc gcctgaaccc ggcgcgacgc gaagccgtgc acgcgaacct ccgcgagatc
     2581 gcgcgcgcgg gccatcccct gctccggcac cccgcggacc gggagcgctg cgcgcggtcg
     2641 gtgttccgcg cctatcatcg ccacctcgcc gaattcctcg cgcagggccg cctcacgccg
     2701 ggcgcgtggg acgcgcgctt ccgcttcgag gggaccgagc acctctatcg cgccgcggcc
     2761 gcggggcggg gcgccgtgat cgcggggtcg cacctgggca actgggagct ggccggtctc
     2821 gccctcgccc gtctgggctt tcgcgtgcac gtggtcacgg gaatccagtg gaatccgctg
     2881 ctccacggcg cggtgcgcga gttgaaggaa cgctccggca tcgaggtgag cacgcccgaa
     2941 gacggattca ccccgctgat cgcgacgctg cgccagggcg ggctcgtggt gctgctggtg
     3001 gacggcgacg tctacacgag gggcctgacc gcgccgttct tcggacgcgc cgtttccttc
     3061 cccgtcggtc cggcgctcct ggcgcgccgc gcgggcgcgc cgcttctcca cgcgcatgcc
     3121 gcccgggagg gcggcgggca ccgcgtcgtg ttcgacggcg ccgatctccc cgaccgcgcg
     3181 ctgtcgctcg gacgcgatct ggcgcgactc accgcgcggg tcgccgcggc gcaggagcgg
     3241 aacatcgccg cccaggtggg cgaatggtgc atcttccgcc ccttcttcgg ggacgcgggg
     3301 gcgccggcgg tcgccggcga cgcgggggcg ccggccgatg ccgcttagga tcgcgatcgt
     3361 gacgcaggcc taccacccca cggtgggcgg cgtcacccag cacgtggaag gaaccgcggc
     3421 ctcgctgcgc gcgcgcggcc atcaggtcac catcgtcacc tcgcgccatc ccggaacgaa
     3481 cggcgccgag ccgggcgtct tccgcctggg ccggaatttc acgctgctct acaacggcgc
     3541 cgacaacaac atcacgctcg gcctgggact ggagggcgcc ctgcgcgagc atttcgagcg
     3601 cggggcctac gacgtcgtgc acgtgcactg cccgctctcg cccagcctgc cgatgctcgc
     3661 gatccgttcg gcgcggcagc cggtcgtggg gacgtttcat tcggtgtccg attcccacgc
     3721 ggcgttccgg atcttccggc cgatcctcct tccctacttc gagcggctcg cgcacgtgat
     3781 cgccgtctcc gaggcggcac gcgacgacgt gctccgcacg ttcacgcgcc cgatctcggt
     3841 ggtgccgaac ggggtcgacc tcgcgcgctt ccgtcccggc gtcgtgccgg tggcgcgcta
     3901 ccgcgacggc cgcgtcaaca ttctcttcgt ggggcggttc gatccgcgaa agggcctccc
     3961 ggagctcctg gaggcctgtg agcggctggc ggagcgagga accgacttcc gcctgatcct
     4021 ggtcggcgac ggccggctcc ggcccggcct ggagcgacgg gcgcgccgct cccttccggg
     4081 gcgcgtcgtc ttcgaggggc aggtgccgca cgagcggctc ccgcagtact acgcgaccgc
     4141 cgacctcttc tgctcgcccg cgcgcggcgc ggagagcttc ggtctcgtgc tcctcgaggc
     4201 gatggcgctc ggcgtcccgg tcgtcgcgag cgacatcccc ggctaccgct gcgtcgtgag
     4261 ccacggcacg gaggggctcc tcacgccgcc gcgcaatccc gcggcgctcg cggacgcgct
     4321 cggggggctg atcgaggatc ccgaacggcg cgcgcggatg ggagccgcag gcgtccgcac
     4381 ggcggcgcgc ttcggatggg accgggtggc gcgcgatctg gagcggatct acgcctccgt
     4441 cctgggcgct cgcggcgagg cgccggagcg ggcggcggcc gaggcgccgg agacgccggc
     4501 cgcggtctga tgggaggagc cgcatcgctc cggctcttcc gcgacacgag cttccgggcc
     4561 ctctggatcg gccaggtcgt ctcggtcttc ggcgaccggt tcacctacct ggcacttctc
     4621 gccgtcgtca tcgagcacgc gcgcaccccc gcgaatcccg cggccgagct ctcctggatt
     4681 ccggtcgtct ccttccttcc cacgatcctg ttcggtccct ggatcggagc gctggtcgac
     4741 acctgggacc gaaagcgcgt cctcgtcctc tccgacgcgg cccgcggcgt ggtcgcgctc
     4801 ctcttcctcg tcgtggtacc ccacgccggc ctcaccgggg ccttcgggct cgtcttcgcg
     4861 ctctacgtcg ccaactcctt cttcctcccg gcccgctccg cgatcctgcc cgaaatggtc
     4921 cccgaggacc ggctcgtcga ggcgaactcg ctcgtcacgc tcgcgggcgt cggcgccacg
     4981 atcgccggat cgctcgccgg gggcgtcgcc atcgcccgct tcggatggag ggtggggttc
     5041 gcgctcgatg cgctcaccta tttcgtctcg gtggtggccc tgctctggat ccgggtcgtg
     5101 ccgagggttg tggtgccgtc agcgccacac ccgccgcaca cggcgggcga tgcctaccgc
     5161 gccctctacg ccgacgtgcg cgagggcgcc cgcatcacgc tgcagagccc ccgcgccctg
     5221 ggcgcgatcg ccgcgacggg gcttctctgg gtggcgggag gagcccttca cgtcgcggcg
     5281 ccgatcctgc tcgcgagccg cggacacggg atcgtcgccg gcgtgggaac gctgctcgcg
     5341 gcgacggcga cgggcatggt ggcgggctcg ctcgcgctgg ccgcgcgcgg cggcgggggc
     5401 tctccctggg gccggacggc ggccgggctc gcgggatcgg gagcggcact cctcctcttc
     5461 gcccgctcgc accgaacggg cgccgatctc gcgctgggct ttggggccgg cgccttcgtg
     5521 gcgctcctcc tggtcaccac ggaggccgcg cttcagggcg cgatcggtcc cgtggcgcgg
     5581 gcgcgcgtct tcgcgctgcg cgacttcacg gctcggctcc tggtgctcgc gtcggcaggc
     5641 ctctgcggcg tgctgctggg gcgcgggatg gtgcggcccg aatgggcggt ggcgggagcg
     5701 ggagccctgc tcatcgcggg ggctttgacg ctggcgttca cggcccgggc aagggatcgc
     5761 gggaagggag tgccggcttg agcgcggcca tcggccgcgc ggacgtctag aagatcttct
     5821 tcccctccgc cacgatctcg ttcagagcgt cgcggaagta cggcgtggag ttggcgaaat
     5881 caaccccgac ccgctccttg taggtctccc acgatttctt gatctcgtag ccgagcaccg
     5941 cgaccaggtt cccgtcgcgg atcccacgct cgcgcttctc ccggttgtag atcgcgatgt
     6001 ccgacgccag cacgcgcgcc agcctccgcg cccgctcgtg ccgctgcagc tcctcgggcg
     6061 agagcccctc gggcagcgtg gtcgcggaaa tgcgcggacg atgagtctcg tggtgcgccg
     6121 cgggttcatg gtgcgcgggc gccgcgggct cgtggtgcgc gggcgccgcg ggctcgtggt
     6181 gaacaggctc acgctgggcg ggcgggggcg cgggcgcgac atgcgtctcc tcgcgcggaa
     6241 tgggagcgaa gaccgccgcg ccggccgcgg gctccgtcgc cgcggactcc ggagttccga
     6301 acagagcttg cgccggcgcc gcggcgtgcg gagccggagc gacgctggtc gaagccgcgg
     6361 gaaagcgctc gaactccgag gccgccggat gcgaaggcgg cgcgtgcgag gggaagcggt
     6421 cgaagccgga atcggccggc tgagacggag tctcgggcgc ggccgaacgc cgcgaaggct
     6481 cgggcgtcgc gggcgccgcc ttgggctcgg ctgccggagg gtgcgcctgc gaaggggctt
     6541 ccacgggagc cggcggtgtc gcggcatgag ccggcgcttc gggcgtgggc ggcacgaccg
     6601 gcagcggctc cggcgtcacc accggcgcgg atggctgcac caggaacacg gcgctgcact
     6661 tccggcagcg cacgcgcgcc ccctgcggcg gcacgagcga atcggcgatc gtgtagcgcg
     6721 cgtggcaggc ggtgcattcg atggtcatgc cgttcgatcc cccggtgccc tgcggtgatt
     6781 cccgtacgat ccgtctatcg gcctgcgacc ccgtatcttt cggggtttcg tggtctaccc
     6841 cccggccagc agatggccgg cgcccgcgac gtgggcctcc acccactccc ggagccgctc
     6901 ctcccgcccg cgctcccgct ccccccgctc gaggagctcc gcccccaggt cccgggcagc
     6961 ctccatcagg tcggagtcgc ccaccggatc ggccacccga agctccggaa acccatgctg
     7021 cgccgtgccc aagagctcgc ccggcccccg aagccggagg tcctcctcgg cgagccggaa
     7081 gccgtcggtc acccgctgga agacgccgac ccgcttccgg gcgagggcgc cggcgtcctt
     7141 cgacagcgcg agcacgcatc gaccgggccg gtccgctcgt cccacccggc cgcggagctg
     7201 gtggagctgg gccagtccga accgctccgg gtgttcgatc acgacgaggg tcgcgtccgg
     7261 aatgtcgagt ccgacctcga ccacggtcgt cgcgaccagg actcgggctt cgccttcgcg
     7321 gaagcgctcg atggcggcgc ggcgctcgtc gggcttcaac cgcccgtgca ccagcgcgac
     7381 gcccgcggcg gccagggccg gcacgcgcgc gagggcggcc gccttcgaga ccgcgtcctt
     7441 cagatcgacc gcctccgatt cctcgacgag aggatagacg acgaaggctc tccctccttc
     7501 ttcgacttcg cgcacgatcc ggtcgaggag cccgcgccgg tcctcgccgc tcacgatcgt
     7561 ggtctcgacc ggcgtgcggc cgggaggctt ctcggtgaga ggcgagagat cgagatcccc
     7621 gtagaccgcc atcgccagcg tgcgcgggat cggcgtcgcg gagaggacga gcagatgggc
     7681 gcccgacgcg cccacgccct tttccgcgag ggcgatcctt tggcgcacgc cgaaacggtg
     7741 ctgctcgtcg acgacgacga agccgagcgc cgggaaggcg acctccttct cgagcagcgc
     7801 gtgcgtcccg atcacgagcg acagcgcgcc cgaatcgagt tcggcccgga tccggtcgcg
     7861 ctcggactgc ggcgtggaag cggtgaggag cgcgtggcgg atcccgctcc cggccagcag
     7921 gcgctccgcc gtcttggcgt gctgctcggc gaggatcgcg gtgggcgcaa gaagcgcggt
     7981 ctggacgccg gcgccggccg cgcgcgcggc gcccgccagc gcgaccacgg tctttcccga
     8041 gcccacgtcg cccaggagca gccggcgcat cgccgcctcc tggtccaggt cctgctcgat
     8101 cgccgcgatg gcttcccgct gggaccgcgt gagcgcgaac gggagcgacg ccaggaagcg
     8161 gcgcatgagg tcggcccctt ccccgagcgg ccgggcgcga cggttcgcgc gatgctcgac
     8221 gcgcgcgagc tgcagcgcga tctgaagacg gagcagctcc tcgagcgcga gacggcgacg
     8281 cgccggctcg acctgatcga gcgattcggg aaagtgaagg cgctcgaacg cgtcctggag
     8341 ccgcgggagc ccggcgagtg cggcgtgatc gcgagcgcgt gagaacgtga cggaggattc
     8401 cgcggcgtcg ggcggaaccg gcgaggagcg cgtgacggcg gaccctcccg tcgtgccggc
     8461 gctcgaatcg tcgagcacga gatccggaac gggcgccagc tcgtccagcg cgtcgcgaac
     8521 gcgggctcga agccaccgct cggtgatccc ctccgtcagg ccgtagcgcg gcacgaggcg
     8581 cgacgtgtgc acgcgctccg actcgttttc gtacggctcg aactcggggt tgtgcagctc
     8641 cacgccgcgg aagggctgcc ccttccccga gaagacgtag cgctcccctt ccacgagctg
     8701 cggcccgcgg aacgcctggt tgaaccagac ggcgcgcgtg aagcccgact cgtcacgaag
     8761 ggaagcgctc aggtcggccc gccccccgcg gcgccggtcc acccgtttgc tgaccaccgt
     8821 cgccacgatg gtggcctcga cccccggctg gagatcggcg atggacgtga agtcgcgccg
     8881 gtcgcgatgc ccggtgggca cgtggaagag gacgtccccc acggtgagga tcccgagccg
     8941 ggcgaggcgc cctgccatcg ccgggccaac ccccttgagg tatcgcaccg gatcggagag
     9001 ggagtgcatt cgtcgagggt agcatgggcg cgggagccgc ttcgctccca cgcgcgcggg
     9061 gccgcagccg gtcgcgagcg gttcgcgcgg gtcgccgcag ccgggcacga gctgctcggc
     9121 cattcgctcg ggagcgagcg ccgcgcgcgc gcggcccata tctcgcgcga cgctcgcgga
     9181 gcagaccggc accgttcagc gcaggacgcg ccgcccgtcg gggccggaag gccagtgcgg
     9241 cgcgcagtcg ctcttcgaag gaggagcact cgagcgtcat cgcgtgatcc gcgacgcggc
     9301 gggcatcgtc cgcgcggcag cccaggccgc gcagcgccgc gatcagatcg ttcgtttcct
     9361 cccgacgggc tttccgggcc gccgcttccc gttcagcggc ttctcgttca gcggcttctc
     9421 gttcggcggc ctgtcgttcg gcagcctgtc gttcggtgac ctgtcgttcg gcagcctgtc
     9481 gttctgcgac ccgtggttcc gccatctctc gctcgacccg ggcgaccctc gcggcatggc
     9541 gcttcagggc ggcatccagg cgcgcgaccc gcgcctgctc gcgcttccct gccatgaacc
     9601 cggctccgaa cgtgtgctcg gctgcgtgct gattgtgggg gcggcaccgg aggcgcaagc
     9661 catcaacggt ggaaatgccg ccgcgcgcga agacgtcgac atggtcgaac tcgaggaagc
     9721 ggcgggacgc gcaccgatgt ccgtccgcga tgagttggga ttgtcgcaaa cgtccaacgc
     9781 gcggccccaa cgcgcggccc caaattgcgg gcccccacgc gcgcccgaaa cgcgcgaccc
     9841 gggcgcgaag ccccgcccca ggcccatcta aggcgcccca ccaccaccac tggacaaccc
     9901 gccccgcctc cgctagactt ccccgctcat atccttaccg gaggaaccat ggcgaagatt
     9961 tgcgacatct gcggcaaggg cgtgcagttc gggcagaacg tgagccacgc gaacaacgtg
    10021 accaagcgcc gctgggaagt gaacttgcag acggtgcggg ccacggtgga agggcgcccg
    10081 aagcggctcc gcgtctgcac acgctgcatc cggaccggac gggtccagaa gagcatgccc
    10141 caggcgcagg cgctccgcgc ccgctcggcc tagccgcacc gccgccgggt ccggggccgc
    10201 aacacgcgcc ccctcgcgca ccgccccacg cgcacgcgca cccggaatcg taaacgcaaa
    10261 cgcccgctcc ttcccggagc gggcgttcgc tttgtcgggc cggggaccc
//
LOCUS       DASSLZ010000316         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1510773, whole genome shotgun sequence.
ACCESSION   DASSLZ010000316 DASSLZ010000000
VERSION     DASSLZ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510773"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VFM00_11955"
     CDS             complement(<1..769)
                     /locus_tag="VFM00_11955"
                     /inference="COORDINATES: protein motif:HMM:NF014951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499004.1"
                     /translation="MTRVALVTSEAFADLYEDDRLLTPALADIGIASSPAVWSDASID
                     WSSFDALIMRSPWDYFERVAEFRAWLDARIAEDVLLCNPREILEWNFDKGYLESLARS
                     GVAVVPTLCVERGDGADIAARARARGWDEVVVKPAIGGGGYRLLRFRLEEFDRHAADI
                     ARTLEDRGVLVQPFLPEIQSGGELALMFFDGVFSHAVRKRPKAGDYRVQFQFGGTHEN
                     ATVGEDLVAQARRCIEHAPAPTVYARVDGVVRDGSFLL"
     gene            complement(766..1905)
                     /locus_tag="VFM00_11960"
     CDS             complement(766..1905)
                     /locus_tag="VFM00_11960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET7499005.1"
                     /translation="MPDFIRAINVEELRGMARKRVPRFVFDFLDGGAEDERTLDANRR
                     SFERLRFRPRTLVGVSGRSMVAPILGSPSALPVVAAPVGLLGLVRHHGDLELARAAAR
                     AGIPFALSTSSMDSMEDVARDASGRLWFQCYVFQERAVSEGLIRRAADAGFEALIITS
                     DFPVSGKRERDLRSGLRPDARFTLGTKLDMLRHPRWLMTVAARRPRFAVVERGIGAGR
                     NAASFLPSKMFDPSLCWDDLRRFRDLWKGKLLLKGVLRADDAALAVECGVDGVILSNH
                     GGRQLDGAISGMDALPDVVREIGGRASILVDGGVRRGGDIAKALALGAEGVLLGRALA
                     YGLAAGGTAGAARALQILSDEFDRTLALLGCRTPGDLAPDRVVAP"
BASE COUNT          345 a          738 c          680 g          263 t
ORIGIN      
        1 tcaagagaaa gctcccgtcc ctgacgacgc cgtcgacccg cgcgtagacc gtcggggccg
       61 gcgcgtgctc gatgcagcgg cgcgcctggg ccacgaggtc ctcgcccacc gtcgcgttct
      121 cgtgggtgcc gccgaattga aattgaacgc ggtagtcgcc ggctttcggt cgcttgcgga
      181 cggcgtggct gaagaccccg tcgaagaaca tcagggcgag ctcgccgccg ctctggatct
      241 cgggcaggaa gggctgcacc agaacccccc gatcctcgag ggtcctcgcg atgtcggcgg
      301 cgtggcggtc gaactcttcg aggcgaaagc gcaggagccg gtagccgccg ccgccgatgg
      361 ccggcttgac cacgacctca tcccagccgc gggcgcgcgc ccgggccgcg atgtcggcgc
      421 catccccccg ttcgacgcaa agggtgggga cgaccgcgac gcccgaccgc gcgaggctct
      481 ccaggtagcc cttgtcgaaa ttccattcga ggatctcgcg cgggttgcag aggagcacat
      541 cctcggcgat ccgcgcatcc agccaggcgc ggaactcggc gacgcgctcg aaatagtccc
      601 agggcgagcg catgatcaag gcatcgaacg aggaccaatc gatgctcgcg tcgctccaca
      661 cggccggtga cgaggcgatg ccgatgtccg cgagcgccgg cgtcagcaga cgatcgtcct
      721 cgtagagatc ggcgaacgcc tcgctggtca ccagcgccac tcgcgtcatg gggcgaccac
      781 ccgatcgggc gcgaggtcgc ccggagtccg gcagcccagg agggccagcg tccggtcgaa
      841 ttcgtcgctc aggatctgca gcgccctcgc cgctccggcc gtgccgccgg cggccagccc
      901 ataggcgagc gcccgaccca ggaggactcc ctccgccccc agggcgagag ccttggcgat
      961 atcgccgccg cgacggacgc cgccatccac caggatcgac gcgcgcccgc cgatctcgcg
     1021 aaccacatcg ggcagggcgt ccatcccgct gatggcgccg tccagctggc ggcccccgtg
     1081 gttcgagaga atgacgccgt cgacgccgca ctccaccgcc agcgcggcgt cgtccgccct
     1141 gaggacgccc ttcagaagga gcttcccttt ccagagatcg cggaagcggc gcaggtcgtc
     1201 ccagcagagc gaggggtcga acatcttgga tggcaggaac gaagcggcgt tccggcccgc
     1261 tccgatgccg cgctcgacga cggcgaagcg tggccggcgg gccgcgacgg tcatgagcca
     1321 tcgagggtgt ctcagcatgt cgagcttcgt tcccagggtg aaccgggcat caggccgaag
     1381 cccggaccgg agatcccgct cccgcttccc agagaccggg aagtcgctcg tgatgatgag
     1441 cgcctcgaag cccgcgtccg cggcccgccg gatcaacccc tcgctcacgg cccgctcctg
     1501 aaagacgtag cactggaacc agagccgccc cgacgcatca cgcgcgacgt cctccatcga
     1561 atccatggag ctggtggaga gggcgaaggg gatgccggcc ctcgccgcgg cgcgggccag
     1621 ttcgagatcg ccgtgatgcc ggaccaggcc gagcagcccc acgggtgccg caaccacggg
     1681 cagagccgac ggagagccga ggatcggagc caccatgctc cgccccgaga cgcccacgag
     1741 cgttcggggg cgaaaacgga gccgctcgaa ggagcggcga ttggcgtcga gcgtccgctc
     1801 gtcctcggcg cctccatcca ggaaatcgaa gacgaaacgc gggacgcgct tccgcgccat
     1861 cccgcgcagc tcctcgacat tgatcgcccg gatgaagtca ggcatggccg gcccaccgca
     1921 gcacgcgcag ggcacgaagg gtgttccacc ggctcggcct gccttccccg tcgtcgatct
     1981 caaccggcat cacgccagga tacctcgtat cgagcgccca ccgccc
//
LOCUS       DASSLZ010000317         6549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_500083, whole genome shotgun sequence.
ACCESSION   DASSLZ010000317 DASSLZ010000000
VERSION     DASSLZ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500083"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFM00_11965"
     CDS             complement(<1..742)
                     /locus_tag="VFM00_11965"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HET7499006.1"
                     /translation="MRALALLPLGLLVVLAVLVPVPAASQYLFLDTNKDGTHDANDRL
                     GPGTTFVDIWIVTNQNRDGTPVSCVVADSGNGLTINSYAVVLHAAGGEVQWGPMQNRL
                     PFSTPTPACFAMYEDTTDTAWYHNGWGWSDQFPPGKHLLATLPIHVVSGDPSIFIEPY
                     LPRHVVQITAFGTSCMGYDGDNTYKLGSEFQDADGLGPLRAEAGGPYQVQAGRTLTVD
                     GTGSRSMVGSPLTYAWNFGDGATATTGVA"
     gene            894..1775
                     /locus_tag="VFM00_11970"
     CDS             894..1775
                     /locus_tag="VFM00_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7499007.1"
                     /translation="MSRARLQVLATALIFSVGGAGIKACALTSWQVASFRSGIATIAV
                     WLMMPEARRGFRRPVWLVSLAYAATVLLFVLANKLTTSANTIFLQSTAPLYILLLAPA
                     LLREKTRAFDWAIIGLIAIGLSFFFVGAEPPLRTAPNPVLGNILAAAAGITWALTVMG
                     LRWIGRAEGASGSTPAVVLAGNAIAFLVALPFALPVEHARALDWVIVSGLGVVQIGVA
                     YVFLVRAMRHVPALEASVLLLLEPALNPLWAWLIHGERPGPWSNAGGLLILGATALKT
                     WLDSPRGFRPRSAPPAP"
     gene            complement(1714..2940)
                     /locus_tag="VFM00_11975"
     CDS             complement(1714..2940)
                     /locus_tag="VFM00_11975"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET7499008.1"
                     /translation="MPARSFRTTRLWRFLLRPVVISVPFALFFTFLDGSKGASFRGYL
                     TASLVFSAIISWCIEANSAWVRPRINAGGRADRGRALALEVASYGVASMVGSLLAAVL
                     LHFTIAPGLLRSAHVFVEVFLWSALFGALFLAVNYAFSFQRRYLTHVIATAEARGREQ
                     QELRTAASIQQALLPPHSHEAATCSATGSSIPCRTIGGDFFDYFELGDGCLAFALGDV
                     AGKGPPAAILAAMAQGILASHVGLDVPPATTVDRVNKALLRRAIEARFATLFYGCLDR
                     AGKLITCNAGHNPGFLFRGDGTMSRLEQGGLMVGAFDFASYAEEESMLAPGDTLVLYS
                     DGVTDAEAASGEQYGEDRLLSCLERSRGGPPGTILQSLLDDVRMFAGGHPPADDVTVL
                     VVRYADGTPGANPATS"
     gene            complement(2951..4306)
                     /locus_tag="VFM00_11980"
     CDS             complement(2951..4306)
                     /locus_tag="VFM00_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7499009.1"
                     /translation="MAEPRVLLVDDEESFRVIAHEELRHAGYSLETAGCLAEARELLA
                     ERAYDVAFLDLRLPDGNGLDLIPEIHGAFPATQVIVLTAFGTVQEAIWAMKQGAFDFL
                     AKPCTLGQMEAVLQRALQVQALARENLALQRDVDRLKPSDRIVGSTAPLREVMGLIGR
                     VAGTDSTVLIRGESGVGKELVARAVHRASRRARRPFVVVDCASLHENLLQSELFGHEK
                     GAYTGAVRLKHGLFEVADRGTLFLDEIGEVTPALQVKLLRVLETGVFRRVGGTSDVSV
                     DVRVIAATNRDLEAMMHAGTFREDLYYRLNVFSIQIPPLRDRREDIPLLVEHFIHSSG
                     VVAKRGVTVSPEAMSVLVRYDWPGNARELQNVIERALILSDGDVLQPSELPIGVRAAP
                     VRNGELANARMTLAEAERRYIRQVLGECNGHRQRAAKILGISERNLYRKLKELEAEAA
                     C"
     gene            complement(4299..5120)
                     /locus_tag="VFM00_11985"
     CDS             complement(4299..5120)
                     /locus_tag="VFM00_11985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7499010.1"
                     /translation="MNPSPAGTPAPAAPVPVAGIVAPLPLVEDRGALERQLSHSERLA
                     SLGILAAGVAHEINNPLASLLAGVEALRRRLLDPQGAASAADATRLLDILERETRRVR
                     DIADKLMLLARPESEKANWFNLNRAVDDTAALLGYQMTVQGIRSQLELAPGVPCIWAR
                     EGAMRSVCLNIMMNALQAMPKGGVLTARTHFSGTAAMLEVDDTGTGVPPEIKDRIWDP
                     FFTTKPPGRGTGLGLSISQSIVARQGGVIRTENLTPHGARFTVELPIQPRGGEDG"
     gene            5419..5853
                     /locus_tag="VFM00_11990"
     CDS             5419..5853
                     /locus_tag="VFM00_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7499011.1"
                     /translation="MKPLISDAGISALRREMDRLFDQFWGSETPVNTYGVWVPSLDIA
                     DKGDAIVVKAEVPGVDPKEIQVSVDDNMLTLSGEKRYEKDEKDEKHYRMERSMGTFTR
                     SVQLPLPVDATNVTAVFKHGLLTIRLPKTKAVKGASIPVKIE"
     gene            complement(5867..6343)
                     /locus_tag="VFM00_11995"
     CDS             complement(5867..6343)
                     /locus_tag="VFM00_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7499012.1"
                     /translation="MAARPVSIRRAGTPEDFALARVLFLEYASSLGFDLGFQDFEREV
                     ATLPGAYAEPEGCLLLATTEAGTAGCIAIRPFASGVCEMKRLYVRPAYRGRGAGRDLA
                     AASIREARARGYRRMRLDTVPGMDAAIALYRALGFRAIEPYRPNPVPGALYMELDL"
     gene            complement(6355..>6549)
                     /locus_tag="VFM00_12000"
     CDS             complement(6355..>6549)
                     /locus_tag="VFM00_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499013.1"
                     /translation="LWREILERRTLAAVAILTGKMRVEGDKLTLLRHAGANRALIHVV
                     ASVPTVFPAAPAAGAPPAAG"
BASE COUNT         1031 a         2235 c         2288 g          995 t
ORIGIN      
        1 tcgcgacccc ggtcgtggcg gtcgcgccat ccccgaaatt ccaggcgtag gtgagcgggg
       61 atccgaccat gctgcgcgat ccggtgccgt ccaccgtgag cgtccggccg gcctgaacct
      121 ggtatggacc gccggcttcg gcccggagcg gaccgagacc gtccgcgtcc tgaaattcgg
      181 atcccagctt gtaggtgttg tcgccgtcgt agcccataca gctcgtcccg aaggccgtga
      241 tctgcacgac gtggcgcggc aggtacggct cgatgaagat cgagggatca cccgagacga
      301 cgtggatggg cagggtcgcg aggagatgct ttcccggcgg gaactgatcg ctccaccccc
      361 agccgttgtg ataccaggcc gtgtcggtgg tgtcctcgta catcgcgaag caggccggcg
      421 tgggcgtgga gaacggaagc cggttctgca tcggccccca ctggacctcg ccccccgccg
      481 cgtggagcac gaccgcgtag gagttgatcg tgagcccgtt gcccgaatcg gcgacgacgc
      541 agctcaccgg cgttccgtca cggttctgat tcgtgacgat ccagatgtcc acgaacgtcg
      601 tccccggccc aagccggtcg ttcgcgtcat gggtgccgtc tttgttggta tcgaggaaca
      661 ggtactgcga tgctgcgggg accggcacga gaaccgcgag aacgacgagg agacccaggg
      721 gcaagagcgc gagagcacgc atgtggcccg ccttcctccg cgcggcttcc ggccagcttg
      781 ccgcgcgact cgaaggtacg ttaggtccgg tcccttccgg cgtcaaggga gtcttgccct
      841 tcttcactag gtaggccttc gggcctctgc tatagaggag ctacgtgaaa ccgatgagcc
      901 gggcacggtt acaggtcctg gcgacggctc tcatcttctc ggtggggggc gccgggatca
      961 aggcgtgcgc cttgaccagc tggcaggtgg cgagcttccg ttcgggaatc gccacgatcg
     1021 cggtctggct gatgatgccg gaggcccgcc gcggctttcg gcgcccggtg tggctggtat
     1081 cgctggccta cgccgcgacc gttctcctct tcgtcctggc gaacaagctc accacgtcgg
     1141 cgaacacgat cttcctccag tcgacggcgc cgctctatat cctgctgctg gcgccggccc
     1201 tcctccgcga gaagacgcgc gcgttcgact gggcgatcat cggactcatc gcgatcggcc
     1261 tctccttctt cttcgtgggc gccgagcctc ccctgcgaac cgcgcccaac cccgtcctcg
     1321 gcaacatcct ggcggccgca gcggggatca cctgggcctt gaccgtgatg gggctgcgct
     1381 ggatcggacg cgcggagggc gcctcgggat ccactcccgc cgtcgtgctc gcgggcaacg
     1441 ccatcgcctt cctggtcgcg ctccccttcg cgcttcccgt ggagcacgcg cgggctctcg
     1501 actgggtgat cgtgtcgggc ctgggggtgg tgcagatcgg cgtggcgtac gtgttcctgg
     1561 tgcgggccat gcgccacgtg ccggcgctgg aggcttcggt gctgcttctc ctcgagccgg
     1621 cgctgaaccc gctctgggcc tggctcattc acggggagcg gccgggcccc tggtccaacg
     1681 cgggcgggct gctcatcctc ggcgccacgg cgctcaagac gtggctggat tcgccccggg
     1741 ggttccgtcc gcgtagcgca ccaccagcac cgtgacgtcg tcggcgggcg gatgaccccc
     1801 cgcgaacatc cgcacgtcgt cgagcagcga ttggaggatc gtccccggag ggcctccccg
     1861 gcttcgctcg agacatgaga gcaggcggtc ttcgccgtac tgctccccgc tggccgcctc
     1921 ggcgtcggtg acgccgtcgc tgtagagcac cagcgtgtct cccggcgcga gcatcgactc
     1981 ctcctcggcg taggacgcga agtcgaaggc gcccaccatc aggccgccct gttccaggcg
     2041 cgacatcgtc ccgtcgccgc ggaacaggaa gcccgggttg tgccccgcgt tgcaggtgat
     2101 cagcttcccc gcccggtcca ggcagccgta gaagagggtc gcgaagcgcg cctcgatcgc
     2161 gcggcgaaga agcgccttgt tgacgcggtc gacggtcgtg gcgggcggca cgtccagccc
     2221 cacgtgcgag gcgaggatcc cctgggccat cgcggccagg atcgccgcgg gcggcccctt
     2281 gcccgcgacg tccccgagcg cgaacgcaag gcaaccgtcg cccagctcga agtagtcgaa
     2341 gaagtcgccg ccgatggtcc ggcagggaat ggacgatccg gtcgcgctac acgtggccgc
     2401 ctcgtgggaa tggggcggca gcagcgcctg ctggatggag gccgccgtgc gcagctcctg
     2461 ctgctcgcgt ccgcgggctt ccgccgtggc gatgacatgc gtcaggtacc ggcgctggaa
     2521 ggagaatgcg taattcaccg ccaggaacag ggcgccgaac agcgcggacc agaggaagac
     2581 ctccacgaag acgtgcgccg accggagaag cccgggggcg atcgtgaaat ggagcaggac
     2641 cgcggcgaga aggctcccca ccatgctcgc cacgccatag ctcgcgacct cgagcgcgag
     2701 ggcgcggccc cgatcggcgc gcccgcccgc gttgatccgg ggccggaccc aggccgagtt
     2761 cgcctcgatg caccacgaga tgatcgccga gaagaccagc gaggcggtca ggtacccgcg
     2821 aaaggacgcc cctttggacc cgtcgaggaa ggtgaagaag agagcgaagg gaacggagat
     2881 gacgaccggg cggaggagga accgccacag acgcgtggtg cggaaggagc gcgcgggcat
     2941 gcgcgcgatc ctagcacgcc gcttcggctt ccagctcctt cagctttcgg tacaggttgc
     3001 gctcgctgat cccgaggatc ttggccgccc gctggcgatg gccgttgcac tcgcccagga
     3061 cctgccggat gtatctccgc tcggcttcgg ccagggtcat ccgggcattc gccagctccc
     3121 cgtttcgaac gggcgccgcg cgcactccga tcgggagctc cgagggctgg agaacgtcgc
     3181 cgtccgagag gatcaacgcg cgctcgatca cgttctgcag ctcgcgcgcg ttccccggcc
     3241 agtcgtagcg caccagcacg ctcatcgcct cggggctcac cgtgacgccg cgcttcgcga
     3301 cgacgccgct cgagtggatg aagtgctcga ctaagagcgg gatgtcctcg cggcggtcgc
     3361 ggagcggcgg gatctggatc gagaaaacgt tcaggcggta gtaaaggtcc tcgcggaagg
     3421 tgcccgcgtg catcatggcc tcgaggtcgc ggttggtcgc cgcgatcacg cgcacgtcca
     3481 ccgagacgtc ggaggtcccg cccacccgcc ggaagacgcc cgtttccagc acgcggagga
     3541 gcttcacctg cagcgccggc gtcacctcgc cgatctcgtc caggaacagc gtgccccggt
     3601 cggcgacctc gaagaggccg tgcttgaggc ggacagcgcc cgtgtaggcg cccttctcgt
     3661 gcccgaagag ctcgctctgg agcaggttct cgtggagcga ggcgcagtcg acgacgacga
     3721 agggccgccg ggcccggcgg ctcgcgcgat ggacggcgcg cgccacgagc tctttcccca
     3781 cgccgctctc gccccggatc aggacggtgg aatcggtgcc cgccacgcgg ccgatcagac
     3841 ccatgacctc acggagcggc gccgtggagc cgacgatccg atcgctcggc ttcagccggt
     3901 cgacgtcgcg ctgcagggcg aggttctcgc gggccagagc ctggacctgg agcgcccgct
     3961 ggagcaccgc ttccatctgt cccagggtgc acggcttcgc caggaaatcg aaggcgccct
     4021 gcttcatcgc ccagatcgcc tcctgtacgg tgccgaaggc ggtgaggacg atcacctgcg
     4081 tcgcggggaa cgctccatgg atctcgggaa tcaggtcgag gccgttgcca tcggggaggc
     4141 gcagatccag gaacgcgacg tcgtaggcac gctccgccag cagctcgcga gcctccgcca
     4201 ggcagccggc ggtctccagc gagtagccgg cgtgccgcag ctcctcgtgc gcgatcacgc
     4261 ggaacgattc ttcatcgtcg accaggagaa cccggggctc agccatcttc tcctccgcga
     4321 ggttggatcg gcagctcgac cgtgaagcgc gcaccgtgcg gcgtgagatt ctcggttcgg
     4381 atcacgcccc cctgccgtgc gacgatgctc tgggagatgg agagccccag ccccgttccc
     4441 cgccccggag gcttcgtcgt gaagaaggga tcccagatgc ggtccttgat ctcgggaggc
     4501 acgcccgtcc cggtgtcgtc gacctcgagc atggccgccg tccccgagaa gtgggtgcgc
     4561 gccgtgagca cgcctccctt cggcatcgcc tggagcgcgt tcatcatgat gttcaggcag
     4621 acgctccgca tcgcgccctc gcgcgcccag atgcacggga ccccgggagc cagctccagc
     4681 tgggagcgga tcccctgcac cgtcatctgg tatccgagca gcgcggccgt gtcgtccacc
     4741 gcgcgattca ggttgaacca gttcgccttc tcggactcgg gccgggcgag gagcatcagc
     4801 ttgtcggcga tgtcgcgtac ccgtcgcgtc tcccgctcca ggatgtcgag cagccgcgtg
     4861 gcgtcggcgg cggaggcggc cccctgggga tcgaggagac gccggcgaag cgcctcgacc
     4921 ccggcgagca gcgaggcgag cgggttgttg atctcgtggg cgacgccggc ggccaggatg
     4981 cccagcgaag cgagacgctc gctgtggctg agctgccgtt ccagggcgcc gcgatcctcc
     5041 acgaggggga ggggtgcgac gatccccgcg accggcaccg gtgcggcggg cgccggcgta
     5101 ccggcaggac tcgggttcat tccggcagaa cttcccactg acatggcggt ctccgttctc
     5161 tgacagattg gcagaggaga cccgcccgcc gatacggtac ctaggtagaa gtccctcccc
     5221 taccggagcc gaagtgcttg gccggtggag ggtccggggc atggggcgga gcggggccgc
     5281 cgcgggaggg tcgcggcaca attcgtgcgg cgctgcactc ccggtcgagg cgacgaccaa
     5341 agcaccgatc gcgatgccgg gaagcgccgc gccgcccggc cgcgcggaac caccatggcg
     5401 tgcaaagggg gagatggaat gaaaccgctc atctcggatg ccgggatctc ggcgcttcgc
     5461 cgggagatgg accggctctt cgaccagttc tggggctcgg aaacgccggt caacacgtac
     5521 ggggtgtggg tcccctcgct cgacatcgcc gacaagggcg acgcgatcgt cgtgaaggct
     5581 gaagtcccgg gcgtggatcc gaaggagatc caggtctcgg tggacgacaa catgctcacg
     5641 ctatcgggcg agaagcggta cgagaaggac gagaaggacg agaaacacta tcgcatggag
     5701 cggtcgatgg gcaccttcac ccggagcgtc cagctcccgc tgcccgtcga tgcgacgaac
     5761 gtcacggccg tcttcaaaca cggtctgctc accatccggc tcccgaaaac gaaggccgtg
     5821 aaaggcgcct cgatcccggt gaagatcgaa tgacgtcccg aagctcctag aggtcgagct
     5881 ccatgtagag ggcgccggga accgggttcg ggcgataggg ctcgatcgcg cgaaaaccaa
     5941 gcgcgcggta tagcgcgatg gccgcgtcca ttcccggtac cgtgtcgagc cgcatgcggc
     6001 gatatccgcg ggcgcgagct tcgcgaatcg acgcggcggc gagatcacgc ccggcgcccc
     6061 ggcctcgata ggccggccgc acgtacagcc gcttcatctc gcacacgccg ctcgcgaagg
     6121 ggcggatcgc gatgcacccc gccgtgcccg cttccgtggt ggccaggagc aggcacccct
     6181 ccggttcggc gtaggctccc ggaagggtcg ccacctcgcg ctcgaaatcc tggaagccca
     6241 gatcgaaccc gagcgatgac gcgtattcga gaaagaggac ccgcgcgagc gcgaagtcct
     6301 ccggagtccc ggcgcggcgg atggagaccg gtcgcgcggc cacggcgcgg cgtgtcagcc
     6361 cgcggcggga ggcgcgccgg cggcgggcgc cgccggaaac acggtcggca cgctcgcgac
     6421 gacgtggatc agagcgcgat tggcgccagc gtgccggagc agggtcagct tgtccccttc
     6481 cacgcgcatc ttgcccgtga ggatcgccac cgccgccagg gtccgccgct cgaggatctc
     6541 ccgccagag
//
LOCUS       DASSLZ010000318        16881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_500635, whole genome shotgun sequence.
ACCESSION   DASSLZ010000318 DASSLZ010000000
VERSION     DASSLZ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 16881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16881
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500635"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFM00_12005"
     CDS             complement(<1..472)
                     /locus_tag="VFM00_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499014.1"
                     /translation="MRRPDFRTPRSSRPARSGAALLAAVSLAVLLAPGSGARAQIPSA
                     SSATPLPAPVPIVESVTPTGYRVRVPIGDAQTFRARIAGYDLTEIRIPGGVDGGAWGQ
                     PSLPTRTVLLRIPWGANPVARGAWSAFRSLGSLEPVPVPHLVTEAKIRDRVAPEA"
     gene            complement(469..645)
                     /locus_tag="VFM00_12010"
     CDS             complement(469..645)
                     /locus_tag="VFM00_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET7499015.1"
                     /translation="MPLSDELLEILVCPQCRGDLVYDRAAERLVCERCRLRYPIVDDI
                     PVMLIEEAERIPES"
     gene            complement(649..1638)
                     /locus_tag="VFM00_12015"
     CDS             complement(649..1638)
                     /locus_tag="VFM00_12015"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HET7499016.1"
                     /translation="MNGTWLDFEKPLLDLERRIEDARGLAQDGDAAAQEEVSRLEKKA
                     DRLRQEIYSKLTRWQRVKLARHPRRPYTLDYLAVMAPEFMELHGDRRFADDPAMVGGI
                     AVVDGLPLMLIGHQKGRDTKENIYRNFGMAHPEGYRKALRLMRLAANFGIPIVTFVDT
                     PGAYPGLGAEERGQSEALARNILEMAHLAVPIVTVVIGEGGSGGALAIAVGDVVMMLE
                     NSIYSVITPEGCAAILWKDATKAEQAAEALKLTAQDLLELKVIDEVIPEPVGGAHRDP
                     EATARRVQEAVLRHIRALIDLPAQELLDRRLEKYMRMGVYLEESAAPAAASGA"
     gene            complement(1652..5140)
                     /locus_tag="VFM00_12020"
     CDS             complement(1652..5140)
                     /locus_tag="VFM00_12020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET7499017.1"
                     /translation="MSAPFVHLHNHSDYSLLDGACRLDRLIDRAKKLGMPAIALTDHG
                     NLFGAVEFYGIAKAAGLKPIIGCEVYVAHGARGEKSRGPDGKGAYDHLVLLAKNREGY
                     RNLMKLSSLGFTEGFHYKPRVDKELLAAHAGGLLCLSACLRGEVPQRVVAGDLEGARA
                     AAGWYRDLYGADSYFFEIQDHGIPDERVSAEGLLELGRRMEIPVVATNDCHYLQREDA
                     EAHEVLLCIQTGKTMLDPDRFRFATDQLYVKTPDEMATLFAHAPEALRNTLVAAERCE
                     LALDTERVQLPSFPLPPDIESEEGYLRRLAEEGLARRYGDAIEDVHRTRLEYELDVIC
                     KVGYARYFLIVRDFTDYARNNGVAVGPGRGSAAGSIVSYCLGITDLDPIRFDLLFERF
                     LNPERVSMPDIDIDFSYETRQKVIDYVVGRYGRESVSQIITFGTMAARAVVRDVGRAI
                     GMTYAEVDRVAKLVPPDLGMTLPKALEIVPELKALPQQGETYARLMRCSLALEGLARH
                     ASTHAAGVLIAPGPLMDYVPVYKSPKGDFTTQFDMNSLEKVGLLKMDFLGLRTLTVLE
                     DAVAHARQATGATIDLKEIPLDDKATFDLLGKAQSVGVFQLESSGMRDLLRKLMPDTF
                     EDIIAINALFRPGPIQSGMIDDFVKRKHGKQKVTYMHPALEPILKNTYGVIAYQDQVM
                     QIANRLAGFTLAQADLLRRAMGKKKPEEMARMKKSFLDGCAERQVPKKTAEDIFDLME
                     KFAGYGFVRAHSAAYAILSYQCAYLKAHHPAAYLAASLTSEVGDTDRIVALVDEARRT
                     GLQVLAPDVNRSKASFTLEGNAIRFGLSAVKNVGVGSVEAIVKARDEGGPFRSLSDFL
                     RRVDTAALNRRVVESLIQAGACDGLEGDRAQLLEAVGDLLAQIQERARSVAKHQESLF
                     GSDPMAAPSDPPLPLIAPWGLEERLRREKEVLGFYFSDHPLGAYRHIIEARATHTTAR
                     LRECREGQEVVLIGLIAAMKPHVDRNKRPMAFVTIEDLRGTVETTLFSDLFERSRAAL
                     VPGTVIEVRGKVNVREEADPKMVLTAVRPVPHPGEGGPIELRIDLVKAPPSVTPEEIR
                     SVLVRHPGESPVYFKVPGAEGADAVTIRARRLLVSPSEELLQALRERLGADAVRVTGP
                     ALDSPSAGGRAEAVPF"
     gene            complement(5249..7795)
                     /locus_tag="VFM00_12025"
     CDS             complement(5249..7795)
                     /locus_tag="VFM00_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET7499018.1"
                     /translation="MEPWWRKRPEWSRPAWLKAPSGPPPRPSWLKLPRPSWLPKGHAR
                     WLIFLLIVAFGFVAYDWFRLSAIGSSQYAGKGWTFPTRVYADWREYRVGDPLDTPTLQ
                     SSLDRARYRRVWARPTDYGQYRIRGTFFEIRLRPFRYPDFAETGTEVLADVRNGRLVS
                     LAQGFQEPRGRGILRIDPELLGEFSNASRERRSYVPIGEIPRHVVLAVVASEDRRFFR
                     HWGLDLIGVFRATLRNMRAGSVVEGGSTINQQLAKNLFLSQERNIFRKFHEALLALLI
                     EMRYPKEKILELYLNQIYLGQRGSWSVCGVEEASLFYFNKHAKELTLPEGAMLAGIIP
                     APNRLSPYRDRGAALARRDQVLADMVDCGWISKEDARRYRRMPMRFGANPPPTTKAPY
                     FVDYVREILSKDIPPLDLSARGYSIFTTIDPEVQESAERAVKDGAREADWRSGRGGAD
                     RDPAQAALVAIDPADGYLRAMVGGRSYAESPYNRAVDSRRQPGSAFKPFVYVAALDSP
                     YQGRRPPLTAATMLEDMPDTFMTPQGPWAPRNYENSYEGHVTAARALARSLNAATVHL
                     AEMVGIPNVARTAHVLGIQSRLREVASLSLGTSEVTPLELTTAYAALANGGVWIRPVA
                     VRAVLDRAGDEVWSPKKEMRRVLRPETAYMATIMLEGPVIYGTAAGIRSQYGFTRPAA
                     GKTGTTDDENDAWFMGYTPQLVAGVWVGCDHNRKLGLTGTQAAVPIWVRFMEEAHRHL
                     PVRDFAAPPGVVEVWIDAQTGYRAGPDCPRVMRVAFASGTEPRTVCPAFHAPSLFQEG
                     DSTSAFDHGDDLEQRPSDDEAQPDDEPPFDQSVPNRPPPDDSNSPDPRGR"
     gene            complement(7864..9633)
                     /locus_tag="VFM00_12030"
     CDS             complement(7864..9633)
                     /locus_tag="VFM00_12030"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7499019.1"
                     /translation="MDCAEAKLQIESYVAGEMPDDRKGPLEEHLAACAECRLDSELTR
                     ASKSQPSEPDPNASPAVFVDSIRPDGAESEADSGADDSGQRPAGSTQAPDGSWTLESI
                     FGAGGAVRAGEAGGKAPGDSATPGETADPPSPAVSAESVFADESESPRSALAERAAAA
                     AAPLPEPDLPSILSGGTGAGASDESPASDAPTWDFEPADVKGSAKPPEGSLFFAEEAL
                     TRTGARKKSGVPKIVLWGAGGLVGVGLLALSIWIAMTVRKPLPEVAGDRHPMRAGTGP
                     AAPGTDTAVTPEPVAPANPATAADPASAANPVSEDASATGRPSAPSEAQAPNIPNSNP
                     AASAPPRGETAPPPTPKALAAAAPVETPHDAASTPAHKPVSSVETATRTASKPPATTK
                     PVAAKPAPSKVAPAAPRPDTSKPWPAAPVIDPGDDEEEPVTPPRATPRPVAPATPSTA
                     SSTSPTASPGTAPGAATPAPSAESSAPPPAPADAVERPIDRLHLATLNAEQNADLTAL
                     RKLRDTWRAFVKTSIGPDRSRAKRELADCLWAIQTLTTKFSDQKEALAAYRDFVLNAP
                     AGGADARTVARMRQLEDAVAESH"
     gene            complement(9778..9975)
                     /locus_tag="VFM00_12035"
     CDS             complement(9778..9975)
                     /locus_tag="VFM00_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7499020.1"
                     /translation="MAYVIVKDICEGESSCVDVCPVDCIKKGEGENAKATTWFFVVAE
                     DCVSCNACLEACPVEGAILPD"
     gene            complement(9994..11079)
                     /gene="nadA"
                     /locus_tag="VFM00_12040"
     CDS             complement(9994..11079)
                     /gene="nadA"
                     /locus_tag="VFM00_12040"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET7499021.1"
                     /translation="MSRQTLPEPYLTLDDEAIAERIVNAKKALGSRLVILGHHYQRDD
                     VIRHADVTGDSFKLARLGAERTDAEYVVFCGVHFMAESADILRAPHQTVILPDLNAGC
                     SMADMAPTGDVLDAGAALERLGVTDVTPVTYMNSTAEIKAFCGERGGAVCTSSNARAV
                     FEWAFRRREKIFFLPDEHLGRNTAMAMGIPAAAIVVWDPMKEQGGLTAEAIEGAKAIL
                     WKGCCSVHTKFQRRHVDERRAEDPGVRILVHPEVPHEVAEAADVVGSTETIIRTLREA
                     PAGSRWAVGTEYNLVRRMAQAMPDKKIVILSQDFCICATMYRISPQNLLWALERLVDG
                     EVVNPIRVPEPTRTWAKAALDRMLQVT"
     gene            11316..13127
                     /locus_tag="VFM00_12045"
     CDS             11316..13127
                     /locus_tag="VFM00_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET7499022.1"
                     /translation="MPNALSHGVNDFNVHVATVNGSGSQTANTTLLRAIFQMGVPVSG
                     KNLFPSNIAGLPTWFTIRVNERGHLARETRNDVLVAMNPETSVPDIAAMGPGSVIVMN
                     QGLPAPKAPEGATVHVIPFDTMAQPLVPDAKLRRLVVNMIYVGVLAQLLGIDRAEVER
                     ALEAQLGRKPKALAMNRAAVDAGFDYAAAHLPPAPFRVERRDKTAGKILIEGNQAAAL
                     GAMFGGVTFVSWYPITPSSSLAEALSGYLERYRRDPKTGQATYAIVQAEDEISAIGMV
                     LGASWAGARAMTTTSGPGLSLMTEFTGYAYYAEIPAVIWDIQRIGPSTGLPTRTSQAD
                     LLSVAFLSHGDTRNVMLFPSSVDDCFDYGMRAFDLAERLQTPVFVLSDLDLGMNVSMA
                     DPFPYPDKPWDRGKVLSAEDLERIGKFERYRDVDGDGIPYRTIPGTKHALAAYFTRGS
                     GHDETARYTESSEAYPRVMDRLAKKWETARTWVPKPVVDEVPGAELAVVAYGSSRWAV
                     EEARERLLADGVKTSSLRIQAYPFTAEVEAFLARHERIYVVEQNRDAQMAALLRMEYP
                     ERAARLRSVLHYDGLPLAASTVVEKIRSGEREEVEVR"
     gene            13124..14230
                     /locus_tag="VFM00_12050"
     CDS             13124..14230
                     /locus_tag="VFM00_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET7499023.1"
                     /translation="MSGGANVPKSNGGGPAGNGPASGTPVIAKNRIGLSAADYKGSKS
                     TLCPGCGHDAITGSIVQAAFELGLEPHRVAKMSGIGCSSKTPAYFIGRSHAFNAVHGR
                     MPSVATGAFLANRELTVLGVSGDGDTASIGLGQFCHLVRRNVPVAYIIENNGVYGLTK
                     GQFSATADVGSRAKSGAVNELMPIDLCAIAIQLGCGFVARSFSGDPKQLRPILKAALS
                     HRGTAVVDVISPCVTFNAHEGSTRSYTAVKEHDAPLHDVTYVPHFENIEVDYAPGESR
                     EVKLHDGSRVVLKKLGEDYDPYDPARALATLNRGREEGKFVTGLLYVNANEAALDKDL
                     ALVDAPLASLTLEQVRPPRAVLDEVMAGLRTGRS"
     gene            complement(14270..15016)
                     /locus_tag="VFM00_12055"
     CDS             complement(14270..15016)
                     /locus_tag="VFM00_12055"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7499024.1"
                     /translation="MSGRWQEPRQFVLGGPLTPAVKTLLIANVAVFLFQYVAQGLAQV
                     RIDRIFGLVPYDVTHHLFLWQLVTYMFLHGGIWHIGFNMLALWMFGTELESLWGTSRF
                     TRFYFITGIGAAICSTLAQWNSFIPTIGASGAIYGLLAAYGILFPDRTILLYFVIPIK
                     AKYFVILMGLLAFWSSVTASGSGVAHIAHLGGMLFGWLYLRYGWGERRVRVGFRMPDL
                     RGWRERRRQEKLKKKFKVYYKDTRGEDDEE"
     gene            complement(15013..16317)
                     /locus_tag="VFM00_12060"
     CDS             complement(15013..16317)
                     /locus_tag="VFM00_12060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HET7499025.1"
                     /translation="MNLCLYEDPDVSAFGPHVLLRPVFALRCGAFTLIEKLVRAFPEA
                     PLHLMVRTEIEEVTRALYPTASIGEPPAEETLFVNARLCMTDDEVLHFLAASPTEASY
                     MSGGTLFAAKVSAPRVAHAARHLRAGEPERAFEEIRMPAEVNAFLARSAADLIRWSPR
                     QIVQDFRYAFQPETVRGKIDEGAHLLEPRAIHVARGARVMAGAVLNAEDGPILIQENA
                     VIEPLAYVEGPAVIGERSRIKAGAQIRGGTSIGPVCKIGGEVEASVFQGYANKQHDGF
                     VGHSFVGEWVNLGAGTITSDLKNNYSTVRTFRSAADWIERRGEDSGQRLLGLTVGDYS
                     KTGIGATFPTGAVVGVGCNVYGTALMPTYVPSFAWGEPGRLVEHAIDAMIETAARAME
                     RRQAELSVPLDSRIRQAFDDTREDRARFLEAQNREPAGSAKP"
     gene            16467..>16881
                     /locus_tag="VFM00_12065"
     CDS             16467..>16881
                     /locus_tag="VFM00_12065"
                     /inference="COORDINATES: protein motif:HMM:NF017568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase domain-containing protein"
                     /protein_id="HET7499026.1"
                     /translation="MSDVKKVRGGATGSPAPPATSRPAEAPDATPAGVAPAGAVPETE
                     APPAKPSGGAGAPGVAAPSAPAKPHAAPPTAAHHAHPGAPHEPSARALRYREHRIPRR
                     HRVFVNRNLRMTQIRAIGFDLDHTLAHYDPTSVEEL"
BASE COUNT         2548 a         5800 c         5977 g         2556 t
ORIGIN      
        1 cggcttcggg agcgacgcgg tcgcggatct tcgcttccgt gacgaggtgc ggcaccggga
       61 ccggctccag cgaacccagg gagcggaagg cggaccaggc gccgcgcgcg acgggattgg
      121 cgccccaggg aatccggagc aacaccgtcc gcgtgggaag cgagggctgt ccccacgcgc
      181 cgccgtcgac gccgcccggg atgcggattt cggtgaggtc gtagcccgcg atccgcgccc
      241 ggaacgtctg ggcgtcaccg atcgggacgc gcacgcggta ccccgtgggt gtcacgcttt
      301 cgacgatggg gaccggcgcc gggagcggcg tcgcggagga cgcggacggg atctgcgcgc
      361 gggccccgga gccgggcgcc aggagaacgg cgagggaaac cgcggccagg agcgcggcgc
      421 cggagcgggc gggccgggag gagcggggcg tgcggaagtc gggacgcctc aagactcggg
      481 aatccgctcg gcttcctcga tcagcatgac cggaatgtcg tccacgatcg ggtatcggag
      541 acgacagcgc tcgcacacca gccgctcggc ggcgcgatcg tagacgaggt ctccgcggca
      601 ttggggacag accaggatct ccagcagttc gtcgctcaac ggcatggctc aggctcccga
      661 ggcggccgcg ggggccgcgc tctcctccag atacaccccc atccgcatgt acttctccag
      721 ccggcgatcg agaagctcct gggccgggag atcgatcagc gcccggatgt ggcgcaggac
      781 cgcttcctgg acgcggcggg cggtcgcctc cggatcgcga tgcgcgccgc cgaccggctc
      841 gggaatcacc tcgtcgatga ccttgagctc gagcagatcc tgagcggtca gcttcagcgc
      901 ctccgcggcc tgctccgcct tggtcgcgtc cttccagagg atggcggcgc acccttccgg
      961 cgtgatcacc gagtagatcg agttctcgag catcatcacg acgtccccca ccgcgatcgc
     1021 gagggcgccg cccgacccgc cctcgccgat caccaccgtg acgatcggca ccgcgaggtg
     1081 ggccatctcc aggatgttcc gcgcgagcgc ctcggactgg ccgcgctcct cggcgcccag
     1141 gccgggatag gcgcccggcg tgtccacgaa ggtcacgatc gggatcccga agttcgcggc
     1201 caggcgcatc aggcgaagcg ctttccggta accctccggg tgcgccatgc cgaagttgcg
     1261 gtagatgttc tccttggtgt cccggccctt ctggtgcccg atgagcatga gcggcagccc
     1321 gtccaccacg gcgatgccgc ccaccatcgc gggatcgtcg gcgaagcggc ggtcgccatg
     1381 cagctccatg aactcgggcg ccatgacggc cagatagtcg agggtgtagg gacggcgcgg
     1441 atggcgcgcc agcttcacgc gctgccaccg ggtcagcttg gagtagatct cctgccggag
     1501 gcggtccgcc ttcttctcca gccgcgagac ctcttcctgg gccgcggcat ccccatcctg
     1561 cgccagaccg cgggcgtcct cgatccgccg ctccagatcg aggagcggct tctcgaaatc
     1621 gagccaggtt ccgttcatgg gtccctccgg gtcagaaggg aacggcctcc gcgcgcccgc
     1681 ccgcgctcgg cgaatcgagc gcgggtccgg tgactctcac ggcgtcggcg ccgaggcgct
     1741 cccgaagcgc ctggagcagc tcctcgctgg gcgagaccag gagccggcgc gcgcggatcg
     1801 tgaccgcgtc cgcgccctcc gctcccggaa ccttgaagta tacggggctt tcgccggggt
     1861 ggcgaaccag caccgagcgg atctcctcgg gggtgaccga gggcggagcc ttgacgaggt
     1921 cgatccggag ctcgatcgga cccccctccc ccgggtgcgg gacggggcgg accgccgtga
     1981 gcaccatctt gggatcggcc tcttcgcgca cgttcacctt gccgcgtacc tcgatcacgg
     2041 tgcccgggac cagcgccgcg cggctccgct cgaagaggtc ggagaacagg gtcgtctcga
     2101 ccgtgccccg cagatcctcg atcgtgacga acgccatcgg ccgcttgttc cggtccacgt
     2161 ggggcttcat cgcggcgatc agtccgatca gcacgacctc ctgcccctcg cggcactcgc
     2221 ggagccgcgc ggtcgtgtgg gtcgcgcgcg cctcgatgat gtgccggtag gcgccgagcg
     2281 gatggtcgga gaaatagaag cccagcacct ccttctcccg ccggagccgc tcttccagac
     2341 cccagggcgc gatcagggga agcggcgggt cggagggcgc cgccatcgga tcggagccga
     2401 agaggctctc ctgatgtttc gcgaccgagc gggcgcgctc ctggatctgc gcgagcaggt
     2461 ccccgaccgc ttcgaggagc tgcgcccggt cgccttcgag gccgtcgcag gcgccggcct
     2521 ggatcaggga ctcgacgacg cgccggttca gggcggcggt gtccacgcgg cgcaggaagt
     2581 cggagagcga gcggaagggc cctccctcgt cgcgcgcctt cacgatcgcc tcgaccgaac
     2641 cgacgccgac gttcttcacg gcggagaggc cgaagcggat ggcgtttccc tccagcgtga
     2701 aggaggcctt gctccggttc acgtccggcg cgagcacctg aagcccggtg cggcgcgcct
     2761 cgtccacgag cgcgacgatc cggtcggtgt cgccgacttc gctggtgagc gacgccgcga
     2821 gatacgcggc cggatggtgc gctttcagat acgcgcactg ataggagagg atcgcgtagg
     2881 ccgcgctgtg ggcccgcacg aagccgtacc ccgcgaactt ctccatgaga tcgaagatgt
     2941 cctcggccgt cttcttcggc acctgccgct cggcgcagcc gtcgaggaac gacttcttca
     3001 tgcgggccat ctcctccggc ttcttcttcc ccatcgcccg ccggagcagg tccgcctgcg
     3061 cgagcgtgaa ccccgccagc cggttcgcga tctgcatcac ctggtcctgg taggcgatga
     3121 cgccgtaggt gttcttcaag atcggctcga gcgccgggtg catgtaggtg accttctgct
     3181 tcccgtgctt gcgcttgacg aagtcgtcga tcatgccgct ctggatgggg ccgggacgga
     3241 agagcgcgtt gatcgcgatg atgtcctcga acgtgtcggg catgagcttc cggaggaggt
     3301 cgcgcatccc cgaggactcc agctggaaca ctccgaccga ctgcgccttc cccagcaggt
     3361 cgaacgtggc cttgtcgtcc agtgggatct ccttgaggtc gatcgtcgcg ccggtcgcct
     3421 gcctcgcgtg cgccaccgcg tcctccagca ccgtcagggt gcggagtccg aggaagtcca
     3481 tcttcaggag gccgaccttc tcgagcgagt tcatgtcgaa ctgggtcgtg aagtccccct
     3541 tcggggactt gtagaccggc acgtagtcca tgagcggccc aggcgcgatg agcacgcccg
     3601 ccgcgtgcgt cgaggcgtgc cgcgccagcc cctccagggc gagcgagcag cgcatcaggc
     3661 gggcgtaggt ctccccctgc tgcgggagcg ccttgagctc ggggacgatc tcgagcgcct
     3721 tcgggagcgt catccccagg tcgggcggta ccagcttggc gacgcgatcc acctcggcat
     3781 aggtcatccc gatcgcgcgc ccgacgtccc ggacgaccgc gcgcgcggcc atggttccga
     3841 acgtgatgat ctggctcacg ctctcccggc cgtagcggcc cacgacgtag tcgatcactt
     3901 tctggcgcgt ctcgtacgag aagtcgatgt cgatgtcggg catgctgacc cgctcgggat
     3961 tcaggaagcg ctcgaagagg aggtcgaacc ggatcggatc gaggtcggtg atcccgagac
     4021 agtaggaaac gatgctcccc gcggccgagc cgcgtcccgg ccccacggcg acgccgttgt
     4081 tccgcgcgta gtcggtgaaa tcgcgcacga tcaggaagta gcgcgcgtag ccgaccttgc
     4141 agatgacgtc cagctcgtac tccaggcgcg tgcgatggac gtcctcgatc gcgtcgccgt
     4201 agcggcgcgc cagcccctct tcggcgaggc gccggagata cccctcctcg ctctcgatat
     4261 cgggcggcag cgggaaggac ggaagctgca cgcgctcggt gtccagcgcc agctcgcagc
     4321 gctcggccgc gaccagcgtg ttccggagcg cctccggggc gtgcgcgaac agggtggcca
     4381 tctcgtcggg ggtcttcacg tacagctgat cggtcgcgaa gcggaaccgg tcgggatcga
     4441 gcatcgtctt gccggtctgg atgcagagga gcacctcgtg cgcctcggcg tcctcgcgct
     4501 gcagatagtg gcagtcgttc gtcgccacga ccgggatctc catccgccgg cccagctcga
     4561 gcagcccttc cgccgagacg cgctcgtcgg ggatgccgtg atcctggatc tcgaagaagt
     4621 aggagtcggc cccgtacagg tcgcggtacc agcccgccgc ggcccgagcc ccttcgaggt
     4681 cgccggcgac gacccgctgc ggcacctcgc cgcgcaggca ggcgctcagg cagaggagcc
     4741 cgccggcgtg ggcggcgaga agctccttgt cgacgcgcgg cttgtagtgg aagccctcgg
     4801 tgaagccgag ggaggagagc ttcatgaggt tgcgatagcc ctcgcggttc ttcgcgagga
     4861 gcaccaggtg gtcgtaggcg cccttgccgt cggggccgcg cgatttctcg ccgcgcgcgc
     4921 cgtgcgccac gtagacctcg cagccgatga tcggcttcag gccggcggcc ttggctatcc
     4981 cgtagaactc gaccgcgccg aagagattgc cgtgatccgt gagcgcgatc gccggcatgc
     5041 cgagcttctt cgcgcggtcg atcaggcggt cgagccggca ggcgccgtcg agaagcgagt
     5101 agtcgctgtg gttatggaga tggacgaagg gcgcgctcat gcgcgcaccg gaggcgtcgc
     5161 ggggattccg tcgggggcac gatccacgat cgcagtatac gcgacacggg acgcgcgggc
     5221 ggcattttcg aggggccgcg ccgagcggtc agcgacctcg agggtcggga gaattggaat
     5281 cgtcgggagg aggacggttc ggcacgcttt gatcgaacgg cggctcgtcg tcgggctgag
     5341 cctcgtcgtc cgaaggacgc tgctccaggt cgtcaccgtg atcgaacgcg ctggtcgaat
     5401 ccccttcctg gaagagcgac ggcgcgtgga aggcgggaca gacggtgcgc ggttcggttc
     5461 ccgacgcgaa cgccacgcgc atgacgcgcg gacagtcggg acccgcgcga tagccggtct
     5521 gcgcgtcgat ccacacctcg acgacgcccg gcggcgcggc gaagtcgcgc accggaagat
     5581 ggcggtgcgc ctcctccatg aagcgtaccc agatcggcac cgccgcctgc gtgccggtca
     5641 gcccgagctt ccggttatgg tcgcagccca cccacacgcc ggccacgagc tgcggcgtgt
     5701 agcccatgaa ccaggcgtcg ttctcatcgt cggtggtccc ggtctttccc gccgcgggcc
     5761 gcgtgaagcc gtactgcgag cggatccccg ccgccgtgcc gtagatgacc ggaccctcca
     5821 gcatgatcgt ggccatgtag gccgtctcgg gccggagcac gcggcgcatc tccttcttcg
     5881 gcgaccagac ttcgtccccg gcgcggtcga gcacggcgcg cacggcgacc gggcggatcc
     5941 agaccccgcc gttggcgagc gccgcgtagg ccgtcgtcag ctcgagcggg gtcacctcgc
     6001 tcgtgcccag ggagagcgag gccacctcgc ggagccggct ctggatcccc agcacgtgcg
     6061 ccgtgcgggc cacgttcgga atccccacca tctccgccag gtggaccgtg gccgcgttca
     6121 gcgagcgcgc gagcgcgcgc gccgcggtga cgtgcccctc gtacgagttc tcgtagttgc
     6181 ggggagccca gggcccctgc ggcgtcatga aggtgtcggg catgtcctcg agcatggtgg
     6241 cggccgtcag cggcggccgg cgcccctgat acggagagtc gagcgcggcc acgtagacga
     6301 acggcttgaa cgcggagccg ggctgccggc gggaatccac cgcgcggttg tagggcgatt
     6361 cggcgtagct ccgtcccccg accatcgcgc gcaggtagcc gtcggcggga tcgatcgcga
     6421 ccagcgcggc ctgggcggga tcccggtcgg cgccgccgcg cccggatcgc cagtcggctt
     6481 cgcgcgcgcc gtctttcacg gcgcgctcgg cgctctcctg cacctcggga tcgatcgtgg
     6541 tgaagatgga gtagccgcgc gcgctcagat ccagcggcgg gatgtccttg gagaggatct
     6601 cccgcacgta gtccacgaag tacggcgcct tcgtggtagg cggcgggttc gcgccgaagc
     6661 gcatcggcat gcggcggtag cggcgcgcgt cctccttgga gatccagccg caatcgacca
     6721 tgtcggcgag cacctggtcg cgccgcgcca gcgcggcccc gcggtcgcgg tagggcgaga
     6781 ggcggttcgg agccgggatg atcccggcga gcatggcccc ctccgggagg gtcagctcct
     6841 tggcgtgctt gttgaagtag aagagcgacg cctcctcgac cccgcagacg ctccagcttc
     6901 cccgctggcc caggtagatc tggttcaggt agagctcgag gatcttctct ttcggatagc
     6961 gcatctcgat caggagcgcg agcagcgcct catggaattt ccggaagatg ttccgctcct
     7021 gcgagaggaa gaggttcttg gcgagctgct ggttgatcgt gcttcccccc tcgaccaccg
     7081 atccggccct catgttgcgg agcgtggcgc ggaagacgcc gatcagatcg aggccccagt
     7141 ggcggaagaa gcgccggtcc tcgctcgcga ccacggccag cacgacgtgg cggggaatct
     7201 ctccgatcgg gacgtagctt cgccgctcgc gggaggcgtt cgagaactcg ccgagaagct
     7261 cggggtcgat gcggaggatg ccgcggccgc gcggctcctg gaatccctgc gccagcgaca
     7321 ccaggcggcc gttccgcacg tccgccagga cttcggtccc ggtctcggca aagtccggat
     7381 agcggaaggg gcgcaggcgg atctcgaaga aggtgccgcg aatccggtac tggccgtagt
     7441 cggtcggccg ggcccagacg cgccgatacc gcgcgcgatc cagcgacgac tggagggtcg
     7501 gcgtgtcgag cggatcgccg acacggtatt ccctccaatc ggcgtagacg cgggtcggga
     7561 aggtccaccc cttccccgcg tactgggacg agccgatcgc ggagagccga aaccagtcgt
     7621 aggcgacgaa tccgaaggcg acgatgagga ggaagatcag ccagcgggcg tggcccttgg
     7681 gaagccagga cgggcgtggg agcttgagcc acgacgggcg aggcggtggc ccgctcggag
     7741 ctttgagcca ggccggccgg gaccactcag gacgtttgcg ccaccacggt tccacgacct
     7801 acgagcctac gggccgggcc ggacagccgt caatcgaagg ggtcgaagcc gatgcggggg
     7861 cgttcagtgc gactcggcga cggcgtcttc cagctggcgc atgcgcgcca cggtgcgcgc
     7921 gtccgccccg ccggccggcg cgttcagcac gaagtcgcgg taggcggcga gcgcttcctt
     7981 ctggtcggag aacttcgtgg tcagggtctg gatggcccag agacagtcgg ccagctcgcg
     8041 cttggcgcgc gagcggtcgg ggccgatgct ggtcttcacg aacgcgcgcc aggtgtcgcg
     8101 cagtttccgg agcgcggtga ggtcggcgtt ctgctccgcg ttcagggtcg cgagatgcag
     8161 ccggtcgatc ggccgctcga ccgcgtcggc gggagcgggc ggaggcgcgg acgattccgc
     8221 tgagggcgcg ggcgtggcgg cgccgggagc ggtgccgggg gaagcggttg ggctcgtgct
     8281 ggaggcggtg gacggcgtcg ccggagccac cggcctgggc gttgcgcgcg gcggcgtgac
     8341 aggctcctct tcgtcatccc ccggatcgat cacgggagcc gcgggccagg gcttcgaggt
     8401 atcgggcctg ggtgcggcgg gcgcgacctt ggaaggcgcg ggcttggcgg ccacgggctt
     8461 ggtcgtcgcg ggcggcttcg aagcggtccg ggtcgccgtc tctacggagg aaaccggctt
     8521 gtgcgccgga gtggacgcgg cgtcgtgcgg cgtttcgaca ggagctgcgg ccgcgagcgc
     8581 cttcggcgtg ggcggcggtg cggtctcgcc tcgcggcggc gcgctcgcag cggggttcga
     8641 gttcggaatg ttcggagcct gcgcttcgga gggcgcgctc gggcgcccgg tggctgaagc
     8701 gtcctcggaa acgggattcg cggccgaggc cggatcggcg gcggtggccg gattcgccgg
     8761 cgcgaccggc tccggcgtga ccgccgtgtc ggttccgggg gcggccgggc cggtgccggc
     8821 gcgcatcggg tgacggtcgc cggcgacttc cggtaaaggc ttccgaaccg tcatggcgat
     8881 ccagatcgag agcgccagga gtcccacccc gaccaggcct cccgctcccc agagaacgat
     8941 cttcggtacg cccgatttct tccgggctcc cgtgcgggtg agggcctcct ccgcgaagaa
     9001 gagacttccc tccggaggct tggcgctgcc cttgacgtcg gcgggctcga aatcccacgt
     9061 gggcgcatcc gaggcgggcg actcgtcgga agcgccggcg ccggtgccgc cggacaggat
     9121 ggagggcaga tcgggctcgg gcagcggcgc ggccgcggcc gcggcgcgct cggcgagagc
     9181 ggatcgcggg ctctcgcttt cgtccgcgaa gaccgattcg gctgaaacgg ccggcgaggg
     9241 cggatccgcg gtctcgcccg gagttgcgga gtcgcccgga gccttccccc cggcttcccc
     9301 ggctcgcacg gctcccccgg ctccaaagat ggactcgagc gtccagctac cgtcaggagc
     9361 ctgagtcgat ccggccgggc gctggcccga atcatccgcc cccgagtcgg cttccgattc
     9421 cgctccatcg ggacggatcg agtccacgaa cacggcgggg gaggcattgg gatcgggctc
     9481 cgagggctgg ctcttggaag cgcgggtcag ctcgctgtcc aggcggcact cggcgcacgc
     9541 ggcgaggtgc tcttccagcg gtcctttccg gtcgtccggc atctcgccgg cgacatagga
     9601 ctcgatctgt aacttggcct cggcgcaatc catgcggaca gactcccggg ttggaacagg
     9661 gcggccggcc cgcgcggaag aagcccgcgg ggcgcgcggt cctagatatc ggtccggcac
     9721 ggctgaatct tgagacgaat ggagatgcgg gaagcgaggg gcgggacggg gcgcggacta
     9781 gtcgggaagg atggctccct cgacggggca ggcctcgagg caggcgttgc agctcacgca
     9841 gtcctcggcc acgacgaaga accaggtcgt ggccttggcg ttctcccctt cgcccttctt
     9901 gatgcagtcc acggggcaga cgtcgacgca ggagctttcg ccttcacaga tatccttcac
     9961 gatcacgtat gccatcgttc tcctccgggt cgctcaggtg acctggagca tgcggtcgag
    10021 agcggctttg gcccaggtgc gcgtgggctc ggggacgcgg atcgggttga cgacctcgcc
    10081 atcgaccagg cgctccagcg cccacaacag attctgcgga gagatccggt acatggtagc
    10141 gcagatgcag aagtcttggg acagaatgac gatcttcttg tccggcatcg cctgcgccat
    10201 ccggcgcacc aggttgtact cggtcccgac cgcccagcgg ctgcccgccg gggcctcgcg
    10261 cagcgtgcgg atgatcgtct ccgtggagcc gacgacgtcc gccgcttcgg cgacctcgtg
    10321 cggcacctcg gggtgaacca ggatccggac gcccgggtcc tcggcgcgac gttcgtccac
    10381 gtgccggcgc tggaacttgg tgtgcacgga gcagcaccct ttccagagga tcgccttggc
    10441 gccctcgatg gcttccgcgg tcagcccacc ctgctccttc atcggatccc agacgacgat
    10501 ggccgcggcg gggatgccca tcgccatggc ggtgttccgc cccaggtgct cgtcgggcag
    10561 gaagaagatc ttctcgcggc ggcggaacgc ccactcgaac acggcgcgcg cattcgagga
    10621 cgtgcagacc gcgcctccgc gctcgccgca gaacgccttg atctcggccg tcgagttcat
    10681 gtacgtcacc ggggtcacgt cggtcacgcc gagccgctcc agcgcggcgc ccgcgtcgag
    10741 cacgtccccg gtcggcgcca tgtcggccat cgagcagccc gcgttcaggt cgggcaggat
    10801 caccgtctgg tggggcgcgc gcaggatgtc cgcgctctcg gccatgaaat ggacgccgca
    10861 gaacacgacg tactcggcat cggtgcgctc ggcgcccagc cgggccagct tgaacgagtc
    10921 ccccgtgacg tcggcatggc ggatcacgtc gtcgcgctgg tagtggtggc ccaggatcac
    10981 gagacgggat ccgagcgcct tcttggcgtt cacgatccgc tcggcgatcg cctcgtcgtc
    11041 cagggtcaga tagggctcgg gtagcgtctg tcggctcatg gctccattct tgacggcgca
    11101 ccggcggggg tcaagggggg ccttttccgc ccggccgccg gcgaatcgct gccgcggtgc
    11161 tccgcgtcca aaaggaatca gcgtcgtgag ttcaggctcc aggcgggctc gccgcgcgct
    11221 tcctcatgcg ccttgaccgg ccgtccgaag catccgagaa tgagatgctc cgagcggccc
    11281 gcccgacgcg aacaatcgaa tcgaaaggaa ccgcagtgcc caacgccttg tctcacgggg
    11341 tcaacgactt caacgttcac gtggccaccg tgaacggctc cgggagccag accgccaata
    11401 cgacgctcct ccgggccatc ttccagatgg gggttccggt ctccggaaag aacctcttcc
    11461 cgtccaacat cgccggcctc cccacctggt tcaccatccg cgtgaacgag cgggggcatc
    11521 tcgcgcgcga gacccggaac gacgtgctcg tggccatgaa cccggagacc tcggtccccg
    11581 acatcgccgc gatgggcccg ggcagcgtca tcgtcatgaa ccaggggctc cccgcgccca
    11641 aggcgcccga gggggccacg gtccacgtga tccccttcga cacgatggcc cagccgctgg
    11701 tccccgacgc caagctgcgc cggctcgtcg tgaacatgat ctacgtgggc gtgctcgcgc
    11761 agctcctggg catcgaccgc gccgaggtcg agcgcgcgct ggaggcgcag ctgggccgga
    11821 agccgaaggc gctcgcgatg aaccgcgccg cggtggacgc cgggttcgac tacgcggcgg
    11881 cacaccttcc gccggcgccc ttccgcgtgg agcgccgcga caagaccgcg ggtaagatcc
    11941 tcatcgaggg caaccaggcg gccgcgctcg gcgcgatgtt cggcggcgtg acgttcgtct
    12001 cctggtatcc catcacgccc tcctccagcc tcgccgaggc cctctccgga tacctcgaac
    12061 gctaccggcg cgacccgaag acgggccagg ccacctacgc catcgtgcag gccgaagacg
    12121 agatctccgc gatcggcatg gtgctcggcg cctcctgggc cggcgcgcgc gcgatgacca
    12181 cgacctccgg ccccggcctc tcgctcatga ccgagttcac cggctatgcc tactacgccg
    12241 agatcccggc cgtgatctgg gacatccagc ggatcggccc cagcacgggg ctgccgacgc
    12301 gcacctcgca ggccgacctc ctgagcgtgg cgttcctctc gcacggcgac acccggaacg
    12361 tgatgctctt cccctcgtcg gtggacgact gcttcgacta cgggatgcgc gccttcgacc
    12421 tcgccgagcg gctccagacc ccggtgttcg tcctgtccga cctcgacctc ggcatgaacg
    12481 tctcgatggc cgaccccttc ccctatcccg acaagccgtg ggaccgcggc aaggtgctct
    12541 cggccgagga tctcgagcgg atcggaaaat tcgagcgcta ccgcgacgtg gacggcgacg
    12601 gcatccccta ccgcacgatc ccggggacca agcacgcgct ggccgcctat ttcacgcgcg
    12661 gctcgggaca cgacgagacg gcgcgctaca ccgaatccag cgaggcctac ccgcgcgtga
    12721 tggaccgtct ggccaagaag tgggagacgg cgcggacgtg ggtgccgaag ccggtcgtgg
    12781 acgaggtgcc gggggcggag ctggccgtcg tcgcctacgg ctcctcgcgc tgggccgtgg
    12841 aggaggctcg cgagcggctt ctcgccgacg gcgtgaagac ctcgtcgctc cgcatccagg
    12901 cctatccctt cacggcggag gtggaggcgt ttctcgcgcg gcacgagcga atctacgtcg
    12961 tggagcagaa ccgcgacgcg cagatggcgg cgctcttgcg catggagtac cccgagcgcg
    13021 cggcgcggct ccggtcggtg ctgcactacg acgggcttcc gctcgcggcg agcaccgtgg
    13081 tggagaagat ccgtagcggc gagcgggagg aggtggaggt ccgatgagcg gcggcgcgaa
    13141 cgttccgaag tcgaacggcg gcggcccggc ggggaatgga cccgcttcgg gaacgcccgt
    13201 gatcgcgaag aaccggatcg gcctcagcgc ggccgactac aaggggtcca agtccacgct
    13261 ctgcccgggc tgcggccacg acgcgatcac cgggagcatc gtccaggcgg ccttcgagct
    13321 ggggctggaa ccgcaccgcg tggcgaagat gtcgggcatc ggctgttcca gcaagacccc
    13381 ggcctatttc atcggccgct cccacgcctt caacgcggtg cacggccgca tgccctccgt
    13441 cgcgacgggc gcgtttctcg ccaaccgcga gctcaccgtg ctcggcgtct cgggcgacgg
    13501 cgacaccgcc tcgatcggac tcggccagtt ctgccacctg gtgcggcgga acgtgccggt
    13561 cgcctacatc atcgagaaca acggcgtcta cgggctgacc aaggggcagt tctcggcgac
    13621 cgccgacgtg gggagccgcg ccaagtcggg cgccgtgaac gagctgatgc cgatcgacct
    13681 ctgcgccatc gcgatccagc tgggatgcgg cttcgtggcg cgctccttct cgggcgatcc
    13741 caagcagctt cggcccatct tgaaggcggc gctgagccat cgcggcaccg ccgtcgtgga
    13801 cgtgatctcc ccctgcgtga cgttcaacgc gcacgagggc tcgacgcgct cctacaccgc
    13861 ggtcaaggag cacgacgccc cgctccacga cgtgacgtac gtcccccact tcgagaacat
    13921 cgaggtggac tacgcgccgg gcgagtcgcg cgaggtgaag ctgcacgacg gctcccgcgt
    13981 cgtcttgaag aagctgggcg aggactatga cccctacgat ccggcccggg cgctggccac
    14041 gctgaaccgg gggcgcgagg aaggaaagtt cgtgacgggg ctgctctacg tgaacgcgaa
    14101 cgaggccgcg ctggacaagg acctggcgct ggtggacgcg cccctcgcgt cgctcacgct
    14161 ggagcaggtg cgtccgccgc gagccgtgct cgacgaggtg atggcggggc ttcgtacggg
    14221 gcgttcgtag gcagggcgcc gcgcgtgcgc ggcgtgggtc gcgggacggc tactcctcgt
    14281 cgtcctcgcc gcgggtgtcc ttgtagtaga ccttgaactt cttcttcagc ttctcctggc
    14341 ggcgccgctc ccgccacccg cgcagatcgg gcatccgaaa gccgacgcgc acccgccgct
    14401 ccccccaccc gtagcgcagg tagagccagc cgaagagcat cccgcccagg tgcgcgatgt
    14461 gggccacgcc gctcccgctc gcggtcacgc tcgaccagaa ggcgagcagc cccatgagaa
    14521 tgacgaagta cttcgccttg atcgggatga cgaagtagag aaggatcgtc cggtccggga
    14581 acaggatccc gtacgcggcg agcagcccgt agatcgcccc cgaggctccg atcgtcggga
    14641 tgaaggagtt ccactgcgcc agagtcgagc agatcgcggc gccgatcccg gtgatgaagt
    14701 agaagcgcgt gaagcggctg gttccccaca acgactccag ctccgtgccg aacatccaga
    14761 gcgcgagcat gttgaacccg atgtgccaga tcccgccgtg gaggaacatg tacgtgacca
    14821 gctgccagag gaacaggtgg tgcgtcacgt cgtagggaac gaggccgaag atccggtcga
    14881 tccgcacctg cgccagcccc tgcgccacgt actggaagag gaacacggcg acgttcgcga
    14941 tcaggagggt cttgaccgcg ggggtcagcg gcccgcccag cacgaactgg cgcggctcct
    15001 gccagcgccc gctcacggct tggcgctccc cgccggctcg cggttctgcg cttccaggaa
    15061 gcgcgcgcgg tcctcccgcg tgtcgtcgaa cgcctggcgg atgcgggagt cgagcggtac
    15121 cgaaagctcc gcctgccggc gttccatggc gcgcgcggcg gtctcgatca tggcgtcgat
    15181 ggcgtgctcg accaggcggc ccggctcccc ccatgcgaac gagggaacgt aggtcggcat
    15241 gagcgcggtg ccgtagacgt tgcacccgac gcccaccacg gcgccggtcg gaaacgtggc
    15301 gccgatgccg gtcttcgagt agtcgccgac cgtgagaccc aagagccgct gccccgagtc
    15361 ctcgccgcgg cgctcgatcc agtcggcggc gctccggaag gtgcgcacgg tggagtagtt
    15421 gttcttgaga tcgctggtga tcgtgcccgc gcccaggttc acccattctc cgacgaagga
    15481 atgtcccacg aagccgtcgt gctgcttgtt cgcgtacccc tggaacaccg acgcctccac
    15541 ctcgcctccg atcttgcaga ccggtccgat cgaggtgccg ccgcggatct gcgcgcccgc
    15601 cttgatccgg gagcgctcgc cgatgacggc aggcccctcc acgtaggcga gcggctcgat
    15661 cacggcgttc tcctggatca ggatgggccc atcctcggcg ttcagcaccg cccccgccat
    15721 gacgcgcgcc ccgcgcgcca cgtggatcgc ccgcggctcc agaaggtgag cgccttcgtc
    15781 gatcttcccc cggacggtct ccggctggaa ggcgtaccgg aagtcctgca cgatctggcg
    15841 cgggctccat cggatgaggt ccgccgccga gcgcgcgagg aaggcgttca cctcggcggg
    15901 catccggatc tcttcgaacg cgcgctccgg ctcgccggcg cgaagatgcc gtgccgcgtg
    15961 cgcgacgcgg ggcgccgaca ctttggcggc gaaaagggtg ccgcccgaca tgtaggacgc
    16021 ctcggtggga gacgcagcga ggaaatggag cacctcgtcg tccgtcatgc agagccgcgc
    16081 gttcacgaaa agggtctctt cggccggcgg ctccccgatc gacgcggtgg gatagagcgc
    16141 gcgcgtcacc tcctcgatct cggtacgcac catgagatgg agcggcgcct cgggaaaggc
    16201 gcgcacgagc ttctcgatga gggtgaaggc gccgcatcga agcgcgaata cgggacggag
    16261 gagtacgtgg ggaccgaatg ccgagacatc cgggtcttcg tagaggcaaa gattcacccg
    16321 ggcagagtac ggacgggccg tgatgccgtc aaggaatggc ggccggccga gcgcggcgcg
    16381 gacgtccacg aatggcggct ggcccggagc gcctccagcg cctacaatcc gcgaaatgag
    16441 tgacttgaag agggtgcgtg gccgaaatga gtgacgtgaa gaaggtgcga ggcggagcga
    16501 ccggttcccc tgcgccgccc gcgacctcgc gacccgctga agcgcccgac gccacgccgg
    16561 ccggcgtcgc gcccgccggg gccgttcccg agaccgaagc gccgccggcg aagccctcgg
    16621 gcggggcggg cgctccgggc gtcgccgcgc cctccgcgcc ggcgaaaccc catgcggcgc
    16681 cgccgacggc ggcccatcac gcgcatcccg gcgctccgca cgagccgtcc gcccgcgcgc
    16741 tccgctatcg cgagcaccgg atcccgcgac gccaccgcgt cttcgtgaat cggaatctgc
    16801 gcatgacgca gatccgcgcc atcggcttcg acctggacca cacccttgcc cactacgacc
    16861 caacgagcgt cgaggagctg g
//
LOCUS       DASSLZ010000319         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1512985, whole genome shotgun sequence.
ACCESSION   DASSLZ010000319 DASSLZ010000000
VERSION     DASSLZ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512985"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFM00_12070"
     CDS             complement(<1..641)
                     /locus_tag="VFM00_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499027.1"
                     /translation="MRAERTSNGHAERTLKIIGIGAVLFLLFVLVKSNTYGPIIEETR
                     IRSVRWRDPHRFAAALEWNRYRLPIGFLTFPDGGTRLNLSDRASFYIGDTDSQVVRLV
                     ARMDKPKSIYSGFGPGIEGWDSTGFVAVITGSRFDRSEVILHYHVTPAGEIRGLSSVP
                     RGTLLPDPNHPWATDYFEASFSRDSIMVVRETGGLSAPVARVNRKTGTLEPVP"
     gene            complement(645..1811)
                     /locus_tag="VFM00_12075"
     CDS             complement(645..1811)
                     /locus_tag="VFM00_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7499028.1"
                     /translation="MARIPFVYNVRNLGQRRVSTALTAIGIALVAWVFIFTLALAGGF
                     EAALRSTGSAENAIVVRSGSTAELTSIVTREMAAAIQSQPEIARAPDGRPLTTAELLV
                     IWNLPRKSGTTTNVVVRGIGPKSAALRPGVRLIAGRMFRPGLDEIVVGKMVAERFQHA
                     SVGDQLKLSGREWNVVGVFDAGGTSYDSEIWGDVELFMPVFDRPVYQSVILRLDDPSH
                     LAALKKRLEADPRMSVAVHREDQFYAAQSGQLATLLRTLGTFVTLIMALGAVFGALNT
                     MYASVGARTKEIGTLRAIGFSRGAVLLSVLIESTLLALLGGVIGCLFALPVRAFTTST
                     MSFATFSELAFRFDVTPMMLAAGLAFSAAMGLVGGFFPARRAASMEVVDALRQA"
     gene            complement(1842..>2091)
                     /locus_tag="VFM00_12080"
     CDS             complement(1842..>2091)
                     /locus_tag="VFM00_12080"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7499029.1"
                     /translation="RLVAAEALLLSVAGGVIGCGIAFLLFRRVDFTAGGFFPNFRVLP
                     ETVGWGLLLAVLMGVLSGLVPAMQAARLQIAGALRKVA"
BASE COUNT          378 a          725 c          704 g          284 t
ORIGIN      
        1 caaggtaccg gttccagggt tccagtcttc cggttcacac gagcgacggg cgccgacaat
       61 ccacccgtct cccggacgac catgatgctg tcccggctga acgacgcttc gaaatagtcg
      121 gtcgcccagg ggtgattcgg atcgggaagg agagtgccgc gcggcacgct ggagagtccc
      181 cgaatctcgc ctgccggcgt cacgtgatag tggaggatca cctcggatcg atcgaatcga
      241 ctccctgtga tgacggccac gaaccccgtc gagtcccagc cctcgatgcc gggcccgaag
      301 ccgctgtaga tcgatttcgg cttgtccatc cgagcgacga gacggacgac ctgggagtcg
      361 gtgtcaccga tgtagaacga cgcgcgatcg ctcaggttga gccgggttcc accatcggga
      421 aacgtcaaga aaccgatggg cagccgatat cggttccatt cgagggcggc ggcaaaccgg
      481 tgcggatctc gccaccgtac ggagcggatg cgggtctctt cgatgatggg accgtacgtg
      541 ttgctcttga cgagcacgaa cagcaggaac aggacagcac cgattccgat gatcttgagc
      601 gtccgctcgg catggccgtt ggacgtccgc tccgcccgca cggcctaggc ctgcctcaac
      661 gcgtccacga cctccatcga cgccgctctc cgcgcgggga agaaaccgcc caccagcccc
      721 atcgccgcgg agaaggcgag gcccgccgcc agcatcatgg gcgtcacgtc gaaccggaag
      781 gccagctcgc tgaacgtggc gaagctcatc gtgctcgtcg tgaaggcgcg gaccgggagc
      841 gcgaacaggc agccgatcac cccgcccagg agggccagga gcgtggactc gatgagcacc
      901 gagaggagta cggcgccgcg gctgaagccg atggcgcgca gcgtgccgat ctccttggtg
      961 cgcgcgccga ccgaggcgta catggtgttc agcgcgccga acacggcgcc caaggccatg
     1021 atcagcgtga cgaacgtgcc cagcgttcgg agcagcgtgg cgagctgacc cgactgcgcg
     1081 gcgtagaact gatcctcgcg gtgcaccgcg acgctcatgc ggggatcggc ctcgaggcgc
     1141 ttcttcagcg cggccaggtg cgagggatcg tccaggcgca ggatcacgct ctgatagacg
     1201 ggccggtcga agaccggcat gaacagctcc acgtcgcccc agatctcgga atcgtaggac
     1261 gtgccgcccg cgtcgaagac gcccaccacg ttccactcgc ggccggagag cttgagctgg
     1321 tcccccacgg aagcgtgctg aaaccgctcg gcgaccatct tccccacgac gatctcgtcc
     1381 aggcccggcc ggaacatgcg ccccgcgatc agacgcacgc cgggtcggag cgcggcggac
     1441 ttgggcccga tgccgcggac cacgacgttg gtcgtggtgc ccgacttgcg cggaagattc
     1501 cagatcacga gcagctccgc cgtggtgagc ggccggccgt ccggcgcgcg cgcgatctcg
     1561 ggctggctct ggatcgcggc ggccatctcg cgggtgacga tgctcgtcag ctccgccgtg
     1621 gacccggagc gcaccacgat cgcgttctcg gccgagcccg tggagcgcag cgccgcctcg
     1681 aacccgcccg cgagcgcgag cgtgaagatg aagacccagg cgacgagcgc gatcccgatc
     1741 gcggtcaggg ccgtgctcac ccgtcgctga cccaggttcc ggacgttgta gacgaaggga
     1801 atccgggcca ctagcgtccc cgctccgcgc gccgcgccat gctaggccac cttccggagc
     1861 gcgcccgcga tctggagccg cgcggcctgc atcgcgggca ccagcccgct cagcacgccc
     1921 atcaggaccg cgagcaagag cccccacccg accgtctccg gcaggacgcg gaagttcgga
     1981 aagaagccgc ccgcggtgaa gtcgacgcgg cggaagagga ggaaggcgat gccgcaaccg
     2041 atcaccccgc ccgccaccga gagaagcagg gcctcggccg ccaccaggcg c
//
LOCUS       DASSLZ010000320        13417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_728604, whole genome shotgun sequence.
ACCESSION   DASSLZ010000320 DASSLZ010000000
VERSION     DASSLZ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13417
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728604"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFM00_12085"
     CDS             complement(<1..555)
                     /locus_tag="VFM00_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499030.1"
                     /translation="MIPDAARDFEDNLMRFVHARGGRPYILVAPVVLSCGGARTRTPD
                     RYSYSPSEWDSLQGGDDFAFEDAGLAAVLDDVRRQWRGESKAFLTGWEAGGHTVWAQA
                     LRHAERWRGVAPVTPNYQRRGLDAGSFSRAAERGRLPIQPFRCGAPKGETAAMIPQVD
                     AQIELALRDAREHGFRPQPIRIVAK"
     gene            812..1288
                     /locus_tag="VFM00_12090"
     CDS             812..1288
                     /locus_tag="VFM00_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET7499031.1"
                     /translation="MPTIKEAAAEFLAQKRIAVTGVSRTPQKHSGNYIYQRLRDRGYT
                     VFAVNPNVDRIEGDVCYHDLRSIPDGVDAVVIGTNAKHAETTMRECADLGIRHVWMHR
                     AFGKGSVSESAADYGRQHGIQVIPGGCPLMFGPAADPAHRVMRVVCRVAGSVPARV"
     gene            complement(1535..2032)
                     /locus_tag="VFM00_12095"
     CDS             complement(1535..2032)
                     /locus_tag="VFM00_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499032.1"
                     /translation="MSAENYLPVLEPEVEWTEAPSPTPIRVAYDEENNAVTFHERESG
                     RLVHAWIPDRPDARAVADLFVTLSAQPWGTQDTLKSAAATVLAICERAPGQVTLQDEL
                     VSILRVNGNRWMTTGELAAEVNKRGRHWKTYRNRITAAKVLLRTKLYARTFQCTGERI
                     RLVSE"
     gene            complement(2163..2576)
                     /locus_tag="VFM00_12100"
     CDS             complement(2163..2576)
                     /locus_tag="VFM00_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499033.1"
                     /translation="MTHSPRYVGHPAPSLVEEGGPVLGWVALAIVVGSTLFYVWVFST
                     TYYTVDAEGLQVRYGPWTRRYPWSDFSAAYWQKGMFASRIGWPSATPCVRLTDAVLLK
                     RKGKWLGLYLTPNDSRAFLARISELAPELTRETVV"
     gene            2718..3212
                     /locus_tag="VFM00_12105"
     CDS             2718..3212
                     /locus_tag="VFM00_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7499034.1"
                     /translation="MERRLRTEIQQSKPFPSLEEEVHVEILLTAQVANRVMATALKPT
                     GLSPSQFNVLRILRGARPDAIPAGRIAERMVNHDPDLTRLLDRLEANGLVEKARDAKD
                     RRVVNVRITKAGVERIDGASKAVTQAVKAALAPVGPRKLGQLADLLEAARAAVREHSE
                     EVSR"
     gene            3242..4123
                     /locus_tag="VFM00_12110"
     CDS             3242..4123
                     /locus_tag="VFM00_12110"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HET7499035.1"
                     /translation="MALSNELILITGATGQQGGAIAHELLVKGRKIRAMTRHPESPKA
                     AALKRLGAEVVAGDLNDAASIERALHGAWGAFALQNTWEAGIQAEEEQGIRFAEIARK
                     AGIHHLVYSSVQSADRKTGIPHFDNKARVEARIRSLGFPSYAILRPVFFMENLTSPWF
                     LPSIQSGALAVGIRPETELQMVAVKDIGQYGAWAFESHASLNGRAIDIAGDELTMPET
                     AAVLSRVAGREIRFVQVPIEEVRKFSDDFAKMLEWFDRVGYNVDIAAQSAESGVRPTP
                     FAEWAAAADWQPAPATR"
     gene            4175..5032
                     /locus_tag="VFM00_12115"
     CDS             4175..5032
                     /locus_tag="VFM00_12115"
                     /inference="COORDINATES: protein motif:HMM:NF037749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5602 domain-containing protein"
                     /protein_id="HET7499036.1"
                     /translation="MNHRVLVAMAITAVLFLPRPAAAHEEDHAGHAAAPAASAPKIVA
                     PAVARTYVGESKPVGNGTVRTWVGVDRKGTPVSVGVTFSETALLGLPMQPPKDDIGWE
                     WNFALPKEVAVAPFDHVAFYWNPRGHIPDGIYNVPHFDIHFFMVPEPQRAEITAMNYD
                     LERCFKLPSQEYIPAGYILPPQTEHRRMGVHWIDPASHEFHGHDFTATLLYGSYNGKV
                     NFIEPMITRTFLETRPDFTAEVKQPAAYAAAGLYPTSYAVKFDGKSHEYVVSLDGLTK
                     REGETASKQ"
     gene            5306..5551
                     /locus_tag="VFM00_12120"
     CDS             5306..5551
                     /locus_tag="VFM00_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499037.1"
                     /translation="MVSLVVCLVCKHPFSWRIAPLERVCAQCGSLHVLKSGIWELKSR
                     GSQAEGPIVVARRQHTARVALMKVLPKTLVANERRSK"
     gene            5548..5757
                     /locus_tag="VFM00_12125"
     CDS             5548..5757
                     /locus_tag="VFM00_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HET7499038.1"
                     /translation="MTRPERMRMKRIRAGSSKGMIRTSVALPEDLHRRFKLASIETGA
                     VVTELLRRAAAEWIEKYRKGRSRNS"
     gene            6122..7837
                     /locus_tag="VFM00_12130"
     CDS             6122..7837
                     /locus_tag="VFM00_12130"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HET7499039.1"
                     /translation="MKVASKVFVLGALLLLAASTAYAAAPVVVAIPDMSVNAGSATTI
                     NVVAYDVDGDNITLTSSLPAYATLNAPTTGTGIVVTTITLNPTASDLGTVTGSVTATA
                     NAEATTESFQLTVNASGTDLAPMVTAPANQTVNEGSNLTFTVTASDAEAITALAAAPL
                     PAGATFTANASYTSGTFSWTPSSSQSGDYNVVFTASNALSGSATTHIHVVDVTGGNTN
                     PDVTAPATESVNEGQMVTFTVTATDVNGDHVTLTATNLPTGASFTDNGNNTGTFSWTP
                     GSTQSGSYTVTFTGNDGHGGTDAASTVITVNDVSGGGGTEATAKLLGAYNVHRKFLCF
                     RIEPVNNGFDVRNVDLTSIRFSFGGSTIDAMTGKTSLDWSCESDSESDGEDRDASAIH
                     KDGGWGDDDCGECDHEDCPTDTTGCTVSLRACFSMSALQGLFGNQSVPDNLVNATITG
                     NLTTGGTFTATMGAPKVAGNGGDKGKGHEKHALHAKATPNPLNPQTKLTFTLSQAGRV
                     RVLVYDARGRYVKTLLDENRYAGAQTLTWDGSDSRSHKVSSGVYMFRIVAPEGQDIQR
                     VTVLK"
     gene            complement(7927..8829)
                     /locus_tag="VFM00_12135"
     CDS             complement(7927..8829)
                     /locus_tag="VFM00_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499040.1"
                     /translation="MKRPVRIALPLSPSAALALLAAALLFAALTLVTAAALAKDLCDE
                     DDNSTMTFGSGKGAYARDVQEMHEPAPRGTWHIDGGDNGAVRIADWDKEEVMICAKIT
                     AWARDKEHAERLLRSIHVESSGGRLRAEGPEQKEGAHWGVSYRIRAPRSMDLDVQTVN
                     GPVTIEGIRGRMSLHTENGPLTLIGAGGDIEGRTTNGPLTVTLTGSRWQGRGLDAETS
                     NGPVQLRIPDGYSADLTTGTQNGPMAGAYVGASAGRRHRHVNVTLGSGGAPIRVVTTN
                     GPIVVNSGSQGHDDADDEDDDDSE"
     gene            8987..9958
                     /locus_tag="VFM00_12140"
     CDS             8987..9958
                     /locus_tag="VFM00_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7499041.1"
                     /translation="MTRIRIFIPIALFALFVSLASILPAAAPAQIYMPGAEPGIPPKD
                     TTLTIRVSRLAPGVYAAKLSYVWTGWVELPDGILVIDSGMADSAGVALADTIRARSPN
                     KPFKYLVLTHAHDDHILGAKAFLSAGATLVAQASLVHDIDSTLGIPENPSKEIGIRDH
                     RRFGTALRPVDVVWLGRPADSRGDLIVHLPKQKVLFAGDLVWNKSIPWLLDPGFDKAG
                     WYASLDSLMAPRYAANVLVPGHGLIAKPGEGIRFTRNYIRDADDKATRLAGWGQTLAE
                     PRKWGYLGPYEGMEFYQEVHFMNMKRLLAEAKGVKTKGRVKVRAMRQ"
     gene            9990..10598
                     /locus_tag="VFM00_12145"
     CDS             9990..10598
                     /locus_tag="VFM00_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499042.1"
                     /translation="MGPAPIRIATPLFVCLLVGAAGCSSSPASLEGRAARRAAERYLA
                     ALAAHDDGVTRSLASCRAGGPAFRGATVLRPAGLESVSVRTLDSLAAAAGDEERRAAL
                     ELAAAGEEIADSLWLRENAASRRARMFGDAVRAVAMLRAGGADSILRVCLIRVRVRWG
                     GRLVGPEPVDREHVLRALAATGGRWIVFSLLPRDRDPWAALF"
     gene            10868..11881
                     /gene="aroF"
                     /locus_tag="VFM00_12150"
     CDS             10868..11881
                     /gene="aroF"
                     /locus_tag="VFM00_12150"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7499043.1"
                     /translation="MVIVLHRDATPEQRHTVEARLRDLGFQVVYSPNGTQVTMAAVGE
                     GTMPPLDEMRSLPGVEEVRRIPSPFKLASRAFRKETTIVRVGDVEIGGKSIILMAGPC
                     TIESEEQLVKTGAAVRAAGARIMRGGAFKPRTSPYSFQGLGEEGLKLMRRVADMHGLL
                     VISEVMDKSQIALMDKYVDIFQVGARNMQNYPLLRELGNVERPVLVKRGLAATVDEWL
                     MSAEYVMSGGNDRVLVCERGIRAYETYTRNTLDLNAVAVAKSLSHLPVIVDPSHAAGI
                     RDKVPPLARAAIAAGADGLLVEVHYDPESAICDGPQSLFPDQFAELTAQIRMIAEAVG
                     RTL"
     gene            12025..>13417
                     /locus_tag="VFM00_12155"
     CDS             12025..>13417
                     /locus_tag="VFM00_12155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7499044.1"
                     /translation="MAYAFIAIFTVYWGAQAFTNHRIGNYAVETDFYWKYGPAARDLA
                     HGTVDIANYDSKGWGYPAVVTAVSAFGFETFRAAQIVALLSAALSLLLLYRLHRSLFG
                     PGLALAGVLILAGDKVFLANTYEVGTDMFFLAVTLASIALLLRSRDPGWRAILASGLL
                     GGWAFSTRYNGLVLWPGALALFLLFRVPSGAPAARWRRAGLYSAAFLAGALPWLWINA
                     VHTGNPLTNSNYVNVGYAVYGEGNWEKFFYGSTRKIHSFADVVLLDPGRFAGAMVKNF
                     VEHLRRDLFELLSPLWGVLAVIGALLMLRDRPGRRVAGYLAFGVLGFLSLVPVFYGAR
                     FSLPLLPFYVALAAWPFASPQVARVLAGVERAFPVRAFAFLVMWIPIVVSAYGWMQDP
                     GNSENVKAGPYDLIQAVDFLKERNAQSALMARKPHAAFLANMKFVPMPDVPTPDSLRA
                     VALREHARFVLISG"
BASE COUNT         2137 a         4577 c         4528 g         2175 t
ORIGIN      
        1 cttcgcgacg atgcgtatcg gttgggggcg gaagccatgc tctcgcgcgt cgcgaagcgc
       61 cagctcgatc tgcgcgtcca cctggggaat cattgccgcg gtttccccct tcggagcgcc
      121 gcagcgaaac ggctggatgg gcagcctgcc gcgttcggcg gctcgggaga acgagcccgc
      181 gtcgaggcct cggcgctggt aattgggcgt gacgggcgcg acaccacgcc aacgctccgc
      241 atggcgcagc gcttgggccc agacagtgtg acctcccgct tcccagccgg tgagaaaggc
      301 tttggattcg ccgcgccact ggcgccgcac atcgtccagg acggcggcga gcccggcatc
      361 ctcgaacgcg aagtcgtcac cgccctggag tgaatcccat tccgaaggcg aataggagta
      421 acggtcgggg gtcctcgtgc gcgcgccccc gcagctcagg actacgggcg cgaccaggat
      481 gtatgggcgg cctccacgcg cgtggacgaa tcgcatcagg ttgtcttcga aatcgcgcgc
      541 ggcatcggga atcacgacca gcaccggcca cgtgcggttc ggcttccacc ccgagggcca
      601 tcgcgcagag ggcaaccacg accgtgagcc ggagcatgtc gcgactctaa cccagccgcc
      661 acccagcgag catggcttac ctgggtgttt cccgagagcg gccgagcgtg cctcaggtag
      721 ccctcggtcc catttcctat ccgcggatgc ctcgctaccg tccatcttgt cggagtctgc
      781 ctgatgcaga ccaggcgagg agggaggagc catgcctacc atcaaggaag cggctgccga
      841 gttcttggcc cagaagcgga tcgcggtgac gggggtttcg cggacgcctc agaagcacag
      901 cgggaactac atctaccagc gcttgcggga tcgcggctac acggtcttcg cggtgaatcc
      961 gaacgtggac cggatcgagg gggacgtttg ctaccacgac ctgcgctcga tcccggacgg
     1021 cgtcgacgcg gtggtgatcg gcacgaatgc caagcacgcc gagacgacca tgcgggagtg
     1081 cgcggacctc gggatcaggc acgtctggat gcatcgggcg ttcgggaagg ggagcgtctc
     1141 ggaatcggcg gcggattacg ggcgtcagca cggcatccag gtcatcccgg ggggctgtcc
     1201 gctcatgttc ggcccggccg cggatccggc acacagggtg atgcgggtcg tgtgtcgggt
     1261 ggcggggagc gtcccggccc gggtctgagc ccccggccgt tcctgacggt cgcgcgcacc
     1321 aacctttcgc ggcgacccgt gtacttccat ttgcatcgca ggctgagccc gcagctgccg
     1381 gtgaccgcgg agctcgcctg cggtcgggtc ttcgatggac ccagggatcc cgccggggca
     1441 aggggggatt ccggcgtgga gtgcggcggg tcgcagctgc tcccgtgcga tccgcccctc
     1501 ccgccgccgc tacgctcgag acttccggtc gaccctactc cgacaccagc cgaatgcgtt
     1561 ccccggtgca ctggaacgtc cgcgcataga gcttcgtgcg gaggagcacc ttggcggcgg
     1621 tgatccggtt tcggtaggtc ttccagtgcc gtccccgctt gttcacctcg gccgccagct
     1681 cgcccgtggt catccaccgg ttcccgttca cgcgaaggat cgagacgagc tcatcctgga
     1741 gggtgacctg cccgggcgcc cgctcgcaga tggcgagcac cgtcgcggcc gcggacttca
     1801 aggtgtcctg cgttccccac ggctgagccg agagcgtgac gaacaggtcg gccacggcgc
     1861 gggcatcggg acggtcgggg atccaggcgt gcaccaggcg gccggactcc cgttcgtgga
     1921 acgtgacggc gttgttctcc tcgtcatagg ccacccggat cggcgtgggc gacggcgctt
     1981 cggtccattc gacttcgggc tcgaggacgg gcaggtagtt ttcggcggac atggtggtcc
     2041 tcctggggcg gggtcgtctg gaatgggctc cggccgggct cggaaggtag ctggtcgggg
     2101 tcctacccgc gccatcgtca ccgatgcggg aaatctttag ccggaacgtc cgtccggaaa
     2161 ggctagacga ccgtttcccg ggtcagttcc ggcgcgagct cggagattct cgccaggaac
     2221 gccctcgagt cgttgggggt caggtagagg ccgagccact tgcccttccg tttcaggagg
     2281 acagcgtcgg tcaggcggac gcacggtgtg gcggagggcc atccgatgcg ggacgcgaac
     2341 atcccctttt gccagtacgc cgcactgaaa tcggaccagg ggtagcggcg cgtccacggg
     2401 ccgtatcgca cctggagtcc ctcagcgtcg accgtgtagt acgtcgtcga gaaaacccac
     2461 acgtagaaga gggtcgaacc cacgacgatc gccagagcga cccagcccag gaccgggccg
     2521 ccctcttcaa ccaggctggg ggcgggatgg cctacgtacc ttggggagtg ggtcatcggt
     2581 cacacctcca gacgcaggta ggaaccctat acacccaggg atggccagaa atgaaagggg
     2641 ggtgctttga cccccgcgat ggcctcgcac caacattcct cttgacaata ttccgcacaa
     2701 cgattattat gcttgccatg gaacgacgcc tgcgcaccga gatccagcag tcgaagccct
     2761 tcccgagcct cgaggaagag gtccatgtcg agatcctgct cacggcccag gtcgcgaatc
     2821 gggtcatggc caccgctctg aagcccacgg gattgagccc ctcgcaattc aacgtcctgc
     2881 gcatcctgcg cggggcccgc ccggacgcga ttccggccgg ccggatcgcc gagcgcatgg
     2941 tgaatcatga ccccgacctc acgcgcctgc tcgaccgcct ggaggcgaac ggcctggtgg
     3001 agaaggcccg cgacgccaag gaccgtcgcg tcgtcaacgt ccggatcacc aaggcgggcg
     3061 tggagcgaat cgacggcgcg tccaaggccg tcacgcaggc cgtgaaagcg gcgctggccc
     3121 cggtgggtcc gcgcaagctg ggccagctgg ccgatctcct cgaagcggcc cgcgcggccg
     3181 tccgcgaaca ttccgaagaa gtctcacgat agacgacgac ccgaaaccgg aggaacgaac
     3241 gatggcactc tcgaacgaac tcatcctgat caccggcgcg acagggcaac agggcggcgc
     3301 gatcgcccac gagctgctcg tcaagggacg gaagatccgc gccatgaccc gccatcccga
     3361 gagcccgaaa gccgcggcgt tgaagcgcct gggcgcggag gtggtcgccg gcgatttgaa
     3421 cgacgccgcc tcgatcgagc gcgcgctcca tggagcgtgg ggcgccttcg ccctgcagaa
     3481 cacgtgggaa gccggcatcc aggccgagga agagcagggg atccgcttcg ccgagatcgc
     3541 gcgcaaggcc ggcatccacc acctcgtcta ttcgtcggtc cagtccgccg accggaagac
     3601 gggaatcccg cacttcgaca acaaggcccg cgtcgaggcc aggatccgct cgctgggctt
     3661 tccctcatac gccatcctcc gcccggtctt cttcatggag aacctgacgt cgccgtggtt
     3721 cctcccgtcc atccagagcg gcgcgctcgc ggtcgggatc cggccggaga cggagctgca
     3781 gatggtcgcc gtgaaggaca tcggccagta cggcgcctgg gccttcgaga gtcacgcctc
     3841 cctgaacggc cgtgcgatcg acatcgccgg cgacgagctg acgatgccgg aaacggccgc
     3901 ggtcctgagc cgtgtcgccg ggcgggaaat ccgcttcgtg caggtcccga tcgaggaggt
     3961 ccggaagttc agcgacgact ttgccaaaat gctcgaatgg ttcgaccggg tcggctataa
     4021 cgtggacatc gccgcccagt ccgccgagag cggcgtgcgt ccgaccccgt tcgccgagtg
     4081 ggcggcggcg gctgattggc agccggcccc cgcgacgcgc tgatcgattc ccaatcccga
     4141 cgttcgaccc cggacccctg caaggaggag acccgtgaac caccgagtgc tcgtcgcgat
     4201 ggccattacg gccgttctgt tccttcctcg gcctgccgcg gcgcatgaag aggaccatgc
     4261 cgggcacgcc gccgcaccgg ccgcgtcggc gcccaagatc gtcgcgccgg cggtcgccag
     4321 gacgtacgtc ggagagagca agccggtcgg caacggcacg gtccgaacgt gggtgggtgt
     4381 ggaccggaag gggacgccgg tctccgtcgg cgtgaccttc tccgagaccg cgctgctcgg
     4441 cctgccgatg cagcccccga aggacgacat cggctgggag tggaacttcg cgctccccaa
     4501 ggaggtcgcc gtggcgccgt tcgaccacgt ggccttctac tggaacccgc gcggccacat
     4561 tccggacggg atctacaacg tcccccactt cgacatccat ttcttcatgg tgcccgagcc
     4621 gcagcgcgcc gagatcacgg cgatgaacta cgacctggag cgctgcttca agctgccctc
     4681 gcaggagtac atcccggcgg ggtatatcct gccgccccag acggagcacc gccggatggg
     4741 cgtgcactgg atcgatccgg cgtcgcacga gttccacggg cacgacttca cggcgacgct
     4801 cctctacggg tcgtacaacg ggaaggtgaa cttcatcgag ccgatgatca cgcgcacctt
     4861 cctcgagacg cgccccgact ttacggccga agtgaagcag ccggccgcgt atgccgccgc
     4921 gggtctctat ccgacctcct acgcggtcaa gttcgacggc aagagccacg agtacgtcgt
     4981 gtccctggac ggactgacga agagggaagg ggagacggct tcgaagcagt gacgcgaacg
     5041 cgccgccaac tttggggcgg gttccgtgta tagccgatgt cccaccgtag agagcgggtc
     5101 gcgactctcc cgtgcggccc gcctatcttt ctttgcccca atacttagac gaaataacac
     5161 agaatccact tgaacctcgc gctgcgctat tgcatcattg atgcacaggg gatcgctccg
     5221 cagtcgtcgt ctccgcagca gacccagcac aactaccgca aggacgcctc gaatcggcca
     5281 gggaagcgct atgcggagct ctcgcatggt ctcgctcgtc gtatgccttg tctgcaagca
     5341 ccccttcagc tggcgcatcg ctcccctcga gcgcgtctgc gcccagtgcg gctcgctcca
     5401 cgtcctgaag tccggcatct gggagctgaa gtcgcggggg agccaggccg agggtcccat
     5461 cgtcgtggcc cggcggcagc acaccgcgcg cgtggcactg atgaaggtcc tacccaagac
     5521 gctcgtcgcg aatgaacggc gttccaagtg acccggcccg agcgcatgcg gatgaagcgg
     5581 atccgggccg gctccagcaa ggggatgatc cgcacctccg tcgcgctgcc cgaagatctg
     5641 caccgccgct tcaagctggc cagcatcgag acgggggccg tggtcaccga gctcctgcga
     5701 cgcgcggcgg cggagtggat cgaaaaatac cgtaaaggac gctctcgcaa cagctagcgc
     5761 cgcccgccgt ccctcggccg ggtcgccgtc ctccgcgacc cggcgtggcc gaacggcaca
     5821 acggggttgt ccctaccttt tttggttgtc gcgcgcccag ggagacgggc atcctttttc
     5881 acgtcccggt tggaccggtg accaccggga cggctccgcc aacgcgcgga gggatcacgt
     5941 cgagcccttc caggccgtcc aggcctccca ctcggcgcct ccgcagcacg gcgaaagccc
     6001 agccatcgtc ccacgcttgg gacgatctcg ttctccgcgc ggctcccgcg cagcgggacc
     6061 gccagaagct ccatcgcggg gcatcgattt ctccgcgcag tcgcggacca aggagggctc
     6121 catgaaggtt gcatccaagg tatttgtctt gggcgcgctc ctgctcctcg ccgcatcgac
     6181 agcctatgcg gccgcgcccg tcgtcgtggc cattcccgat atgtccgtca acgccggctc
     6241 ggcgacgacc atcaacgtgg tcgcctacga cgtcgacggc gacaacatca cgctcacctc
     6301 atccctgccg gcctacgcca cgctgaacgc gccgacgacc ggcaccggca tcgtcgttac
     6361 caccatcacc ctgaatccca ccgcgagcga tctcggcacg gtgaccggca gcgtcaccgc
     6421 caccgcgaac gccgaggcga cgaccgagtc gttccagctc acggtcaacg cctccggcac
     6481 ggacctggcg ccgatggtca ccgctcccgc caaccagacg gtgaacgagg gctcgaacct
     6541 cacgttcacc gtcacggcaa gcgatgccga ggcgatcacg gccctggccg cggccccgct
     6601 cccggcgggc gcgaccttca ccgccaacgc gagctacacc tcggggacct tctcctggac
     6661 ccccagctcg tcccaatcgg gcgactacaa cgtcgtcttc accgcgtcca acgcgctctc
     6721 cggctcggcg accacccaca ttcatgtcgt ggacgtcacc ggagggaaca ccaaccccga
     6781 cgtcacggct ccggcgacgg agagcgtgaa cgaagggcag atggtcactt tcacggtgac
     6841 cgcaacggat gtgaacggcg atcatgtcac cctgacggcg accaaccttc ccacgggagc
     6901 gtcgttcacc gacaacggca acaacacggg taccttcagc tggaccccgg gatccacgca
     6961 gtccggcagc tacacggtga cctttacggg caatgacgga catggcggaa cggatgccgc
     7021 gagcaccgtc atcaccgtga acgatgtctc gggcggcggc ggcacggaag ccaccgcgaa
     7081 gcttctcggg gcctacaacg tgcatcggaa gttcctctgc ttccggatcg aaccggtgaa
     7141 caacggcttc gacgtccgga acgtggacct gacctcgatc cggttctcct tcggcggctc
     7201 gacgatcgat gccatgacgg ggaagaccag cctggattgg agctgtgaga gcgacagcga
     7261 gtccgacggc gaggatcggg acgcgtccgc gattcacaag gacggcggct ggggcgacga
     7321 cgactgcggc gaatgcgatc acgaagactg cccgaccgac acgaccggtt gcacggtgtc
     7381 cctgcgggcc tgcttctcga tgagcgcgct gcagggcctg ttcggcaatc agagcgtccc
     7441 ggacaatctg gtcaacgcga cgatcaccgg caacctgacc accggcggga cgttcacggc
     7501 gacgatgggt gcgccgaagg tggcggggaa cggcggcgac aaggggaagg ggcacgagaa
     7561 gcacgccctg cacgccaagg cgacgccgaa tccgctcaat ccgcagacca agctgacctt
     7621 cacgctctcg caggcgggac gcgtgcgggt gctggtctac gacgcgcgcg gccgttacgt
     7681 gaagacgctg ctcgacgaaa accgctacgc gggcgcccag acactcacgt gggacggctc
     7741 cgattcccgg agccacaagg tctcgtcggg cgtgtacatg ttccggatcg tggcgcccga
     7801 ggggcaggac atccagcggg tcacggttct gaagtaaacg gagggatcgc cggcctgtcg
     7861 ggcggcgaga cgaacgggcg gggcgcaccg gcgtcccgcc cgtttcgttt gccccgcggt
     7921 cggcggctac tcgctgtcgt cgtcgtcctc atcgtcggcg tcgtcatgtc cctgggagcc
     7981 cgagttcacg acgatcggtc cattcgtggt gacgacgcgg atcggggcgc cgccggagcc
     8041 gagggtcacg ttgacgtggc gatggcggcg gccggcgctg gcgccgacgt aggcgcccgc
     8101 catcggtccg ttctgggtgc cggtggtgag atcggccgag tatccatcgg gaatccggag
     8161 ctgcaccgga ccgttggagg tctcggcgtc gaggccgcgc ccctgccagc gggagccggt
     8221 cagggtgacc gtgagcggac cgttggtggt gcgtccctcg atgtctccgc ccgcgccgat
     8281 gagcgtgagc gggccgttct ccgtgtgcag gctcatgcgg ccgcggatcc cttcgatcgt
     8341 cacggggccg ttcacggtct gcacgtcgag atccatcgag cgcggagcgc gaatccggta
     8401 ggagacgccc cagtgtgcgc cttccttctg ctcggggccc tcggcgcgga gccggccgcc
     8461 ggagctctcg acgtggatgg agcggaggag ccgctcggcg tgctccttgt cccgcgccca
     8521 cgccgtgatc ttggcgcaga tcatcacttc ttccttgtcc cagtcggcga tccggacggc
     8581 gccgttgtcg ccgccgtcga tgtgccacgt accccggggc gccggctcgt gcatctcctg
     8641 gacgtcgcgg gcgtaggccc ccttcccgct tccgaacgtc atggtcgagt tgtcgtcctc
     8701 gtcgcagagg tccttggcga gagcagcggc agtgacgagg gtgagggccg cgaacaggag
     8761 ggcggccgcg agcagcgcaa gagcggccga gggggagagg ggcagcgcga ttcgaaccgg
     8821 gcgtttcatg gcggacctcc tcgagtggat ggcgacctgg gcgtcccttt agaacgccgc
     8881 cgccccgcgg acgttggaat tgcgatacaa tcgtggaccc gacaacgcca tcccggggta
     8941 gtcccgaccg tcacggtcga tcctccccca acgccgacgg agacccatga cccgcattcg
     9001 catcttcatc cctatcgcgc tcttcgccct ttttgtttca ttggcttcga tcctcccggc
     9061 cgccgccccg gctcagatct acatgcccgg cgcggaaccg ggcattccgc ccaaggacac
     9121 gaccctcacg atccgggtga gccgcctggc tcccggcgtc tacgccgcca agctcagcta
     9181 cgtctggacc ggctgggtgg agcttccgga cgggatcctg gtcatcgatt ccggcatggc
     9241 ggacagcgcc ggcgtggcgc tggccgacac gatccgcgcg cgctctccga acaagccgtt
     9301 caagtacctg gtcctgactc acgcgcacga cgaccacatc ctgggcgcca aggcgttcct
     9361 gtcggcggga gcgacgctgg tggcgcaggc gagcctggtc cacgacatcg actccacgct
     9421 gggaatcccg gagaacccgt cgaaggagat cgggatccgc gaccacaggc gcttcggcac
     9481 cgcgcttcgt cccgtggacg tggtgtggct gggacgcccc gcggattcgc gaggcgatct
     9541 catcgtgcat cttccgaagc agaaggtcct gttcgcggga gacctggtct ggaacaaatc
     9601 gatcccgtgg ctcctggacc ccggcttcga caaggccggc tggtacgcct cgctggattc
     9661 gctcatggcg ccgcgctacg cagccaacgt gctcgttccg gggcatggac tgatcgccaa
     9721 gccgggcgag ggaatccgct tcacgcggaa ctacatccgc gacgccgacg acaaggcgac
     9781 ccgcctcgcc gggtggggcc agacgctggc cgagccgcgc aagtgggggt acctgggacc
     9841 ctacgagggg atggagttct accaggaagt ccacttcatg aacatgaagc gccttctcgc
     9901 cgaggcgaaa ggcgtcaaga ccaaggggcg tgtcaaggtt cgggcgatgc gccagtagcc
     9961 gcgcgttcca cgcaggtacg cggcgtcgct tgggtccggc cccgatccgg atcgcgacgc
    10021 cgctttttgt ttgccttctg gtgggggcgg ccggctgctc gtcgagcccg gcttcgctcg
    10081 aggggcgcgc ggcgcgtcgg gccgccgagc gctatctcgc cgcgctcgcg gcccacgatg
    10141 acggggtgac gcggtctctt gcctcctgcc gagcgggtgg tccggcgttt cgcggcgcga
    10201 ccgtccttcg tcccgcgggt ttggaatcgg tctcggtccg aacgctggat tcgctggcgg
    10261 cggcggccgg cgacgaggag cggagagccg ctctcgagct cgcggccgcg ggggaagaga
    10321 tcgcggactc gctctggctc cgggagaacg cagcatcccg gcgcgcccgg atgtttggcg
    10381 acgccgtccg cgcggtggcc atgttgcggg cggggggtgc ggattcgatt ctccgggtct
    10441 gcctgatccg cgtccgcgtg cgctggggag gtcggttggt gggaccggag ccggtggacc
    10501 gagagcacgt cctgcgcgcg ctggcagcca cgggcgggcg ctggatcgtc ttctcgctcc
    10561 tgccccgcga ccgggaccct tgggcagccc tcttttgagc cgcgccgggg agacgcgatc
    10621 cgctctgggg tgacgatttg acccgcgcct ggggatgatg ctaagtttct ggtcccacgc
    10681 acgatcccga aagcacttac gacgaggtgg cgaggcgccg atgaacgtga agaccggctt
    10741 ccattttgga tactgggcgt gggcccgggg gcttgggcgc gagcggggat gaacgatccc
    10801 ggcccgcgcc gaggccccgc ccattgccgg atctccggca gtgggcgttt ctattcctga
    10861 ggatgccatg gtcatcgtcc ttcatcgcga cgccacgccc gagcagcgcc acacggtcga
    10921 agcgcgcctg cgcgacctgg ggttccaggt cgtctactcc cccaacggga cgcaggtgac
    10981 gatggcggcc gtgggcgagg ggaccatgcc gccgctcgac gagatgcgct ccctccccgg
    11041 agtcgaggag gtccgccgca tcccctctcc cttcaagctc gcgagccgcg cgttccgcaa
    11101 ggagacgacg atcgtgcgcg tgggggacgt ggagatcggc gggaagtcga tcatcctgat
    11161 ggccggcccc tgcaccatcg aatccgaaga gcaattggtg aagaccggcg ccgcggtgcg
    11221 cgccgcgggc gcgcgcatca tgcgcggcgg ggccttcaag ccgcgcacct ccccctattc
    11281 gttccagggc cttggggaag aggggctcaa gctgatgcgg cgcgtcgccg acatgcacgg
    11341 gctcctcgtg atctccgagg taatggacaa gagccagatc gccctcatgg acaagtacgt
    11401 cgacatcttc caggtgggcg cgcggaacat gcagaactac ccgctgctcc gcgagctggg
    11461 gaacgtggag cgcccggtgc tggtgaagcg gggcctggcg gcgaccgtgg acgagtggct
    11521 gatgagcgcc gagtacgtga tgtcgggcgg gaatgaccgc gtgctggtct gcgagcgcgg
    11581 catccgcgcg tacgagacct acacccggaa caccctcgac ctgaacgcgg tcgcggtcgc
    11641 caagtcgctc agccacctcc ccgtgatcgt ggatccgagc catgcggcgg ggatccgcga
    11701 caaggtaccg ccgctcgcgc gcgccgcgat cgccgcgggc gccgacgggt tgctcgtcga
    11761 ggtgcactac gaccccgaga gcgccatctg cgacggcccg cagtcgctct tcccggatca
    11821 gttcgcggag ctcaccgcgc agatacgcat gatcgccgag gcggtggggc gcacgctgtg
    11881 acgcgccgcc ggcgcggcgg cccggccccg cggcgcgaag gatcgcgagg tcctggagcg
    11941 ccgcgcggcg aggggcacgg ctcggcgggc ccccacgccc gcgcgcaggg cggagcgacc
    12001 ggagccaccc gctggacccg atgggtggcg tacgccttca tcgcgatctt cacggtctac
    12061 tggggcgcgc aggcgttcac gaaccaccgg atcggcaact acgccgtcga gaccgacttc
    12121 tactggaagt acggtcccgc cgcgcgcgat ctcgcccatg gcacggtgga catcgcgaac
    12181 tacgattcga agggatgggg ctatccggct gtcgtgacgg cggtctcggc gttcggcttc
    12241 gagaccttcc gcgccgcgca gatcgtggcg ctgctgtcgg cggcgctctc gctgcttctg
    12301 ttgtatcgcc tgcaccggag cctcttcggc cccggcctcg cgctcgcggg agtattgatc
    12361 ctcgcggggg acaaggtctt cctggcgaac acctacgagg tcggcaccga catgttcttc
    12421 ctcgcggtca cgctcgcctc gatcgcgctt ttgctgcgga gccgcgatcc gggatggcgc
    12481 gcgatcctcg cgtcggggct gctcggcggg tgggccttct cgacgcgcta caacgggctc
    12541 gtgctctggc cgggcgcgct cgcgctcttt ctcttgttcc gcgtgccgtc gggggcaccg
    12601 gcggcccgtt ggcgccgcgc cggtttgtat tcggcggcat ttctcgcggg cgccttgccg
    12661 tggctctgga tcaacgccgt gcacacgggg aacccgctca ccaactccaa ctacgtaaac
    12721 gtcggttacg ccgtgtatgg agaagggaac tgggagaagt tcttctacgg aagcacgcgg
    12781 aagatccact cgttcgcgga cgtggtcctc ctcgatcccg gccgctttgc cggcgcgatg
    12841 gtgaagaact tcgtcgagca cctccggcgc gacctcttcg agctgctctc gcccttgtgg
    12901 ggcgtcctgg ccgtgatcgg cgctttgctg atgctccggg atcgccccgg ccgccgcgtc
    12961 gcgggctacc tcgcgttcgg cgttcttggg ttcctctctt tggtcccggt cttctatggc
    13021 gcgcgcttct cgctcccgct cttgccgttc tacgtcgcgc tcgcggcgtg gcccttcgcc
    13081 tcgccccagg tcgcgcgcgt actggccggc gtggagcgcg cgtttccggt ccgcgccttc
    13141 gcgttcctcg tcatgtggat cccgatcgtc gtgtccgcct acggctggat gcaggacccc
    13201 ggcaactcgg agaacgtgaa ggcgggcccc tacgacctga tccaggccgt ggactttctc
    13261 aaggagcgaa acgcccagag cgcgctgatg gcccgcaaac cccacgccgc gttcctcgcg
    13321 aacatgaagt tcgtcccgat gcccgacgtg cccacgcccg attcgctgcg cgccgtcgcg
    13381 ctgcgcgagc acgcccgctt tgttctgatc tccggca
//
LOCUS       DASSLZ010000321         2771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1860363, whole genome shotgun sequence.
ACCESSION   DASSLZ010000321 DASSLZ010000000
VERSION     DASSLZ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860363"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1898
                     /locus_tag="VFM00_12160"
     CDS             210..1898
                     /locus_tag="VFM00_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7499045.1"
                     /translation="MTPRIRLSGAQLLTGAVFLAVVAALAVPALVEHERRKGNERSEK
                     ARHSPAAHAATTFRFYQEDPSTLDPALASDSYSSSIVAQIYSPLVGLTSDLEPTPQIA
                     ESWTISRDGLRYVFHIRPGVRFHNGREVTAQDFEYSLTRVFREPFRSEGLAANYLDAI
                     AGVAEFTRGKASRIRGIRALDKSRLEITLTRPYTALLTALALDQTSAVPREIAGKGRE
                     ALERHPVGTGPFRFVRRDAHQIVLAADPGYFMGPPGIDTLVFFAPPGDVTAQGADALL
                     DGHATLSQLPLSRIEEFRARPGIAVLKWNDLSLAFIGMNARIRPLDDPRVRRAIALAM
                     DRQAMLASSPEGKSLATGILPPGLPGYGPSQKAYMRDVGAARALLAQAGYGPAHPLPP
                     LTLWKSSSSVSGRQADTVMVRSLAEAGIRVGLRYESWGVLDSMITGRKAALFALAWVA
                     DIPDPDTFLRALGYSTSSTNYFGFHNRGVDSLLDVARNSADPDERMAAYRKAEESIVR
                     EAPFIPLYHTASFIGIRDDVLGLEMNPLGISTIQMEKLRFTGPDVAFDPRSAAR"
     gene            1895..>2771
                     /locus_tag="VFM00_12165"
     CDS             1895..>2771
                     /locus_tag="VFM00_12165"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET7499046.1"
                     /translation="MTRVAEVEPAAAPRRPRAGLQARIFLTFGIIVVGLVAAAVTFFV
                     RSESDSLLRETRKRGFAVARSIAWLSTPSLLSYNYIALNQAARRSQAGGEIAYVVIYD
                     KEGQIAADSRVRNSFGQPPRDADDYAAHAVREERWKFIPPAAPGRPRVLEILLPVYVD
                     GYQVKWGTVRVGVSLESMDKEIAALGQHLVVAGILAAILCLFAARAAARGITRPIDRL
                     VSATRAVAKGDYSQRVNLQTGDELETLAWHFDRMSDEVQRQQAEVLASRENLARLNQS
                     LEAAVEARTHALAESE"
BASE COUNT          400 a         1043 c          920 g          408 t
ORIGIN      
        1 cgtcgaggcg cggctccagg cgaagcgccg ccggagcgcg gtcaaacgcg cccgttcggg
       61 gccgggagga ggcgccgagg actagggggg tctccctgta aataggctgc aatatagcta
      121 gttacccgct tggatcggtc ccgcctcttt ctccgctcca cccgcggcca gtctcaagaa
      181 cccgcgtcac cgcaccgata gataaccaca tgactccccg gatccgcctt tccggcgctc
      241 agctcctgac gggcgccgtg ttcctcgccg tggtggccgc gctcgccgtt cccgcgctcg
      301 tggagcacga gcgccggaag gggaacgagc gttcggagaa ggccaggcat tccccggccg
      361 ctcacgcggc cacgacgttc cgcttctacc aggaagaccc gagcaccctg gatccggcgc
      421 tggcctccga ctcctactcc tccagcatcg tcgcccagat ctacagcccg ctcgtgggcc
      481 tcacctccga tctcgagccg acgccgcaga tcgccgagag ctggaccatc tcccgcgacg
      541 gcctgcgcta cgtgttccac atccgtcccg gcgtgcgctt ccacaacggg cgcgaggtga
      601 cggcccagga tttcgagtac tcgctgacgc gcgtcttccg cgagccgttc cggtcggaag
      661 ggctcgccgc caactacctc gacgcgatcg cgggggtcgc ggagttcacc cgcggcaagg
      721 cgagccgcat tcgcggcatc cgcgcgctcg acaagagccg cctcgagatc acgctgacgc
      781 ggccctacac cgcactcctc accgcgctcg ccctcgacca gacctcggcc gtcccgcggg
      841 agatcgccgg caagggacgg gaagcgctcg aacgtcaccc ggtggggacc gggccgttcc
      901 ggttcgtgcg gcgcgacgcg caccagatcg tgctggcggc cgatcccggc tatttcatgg
      961 ggccgcccgg gatcgacacc ctcgtcttct tcgcgcctcc gggcgacgtg accgcccagg
     1021 gcgccgacgc cctcctggac gggcatgcca ccctgagcca gcttccactc tcgcgcatcg
     1081 aggagttccg cgcgcgcccg gggatcgcgg tcctcaagtg gaacgacctg agcctcgcct
     1141 tcatcggcat gaacgcccgg atccgcccgc tcgacgatcc gcgcgtgcgc cgcgccatcg
     1201 cgctcgcgat ggaccggcag gcgatgctgg cctccagtcc cgaggggaaa tcgctcgcga
     1261 cggggatcct gccgccgggg ctgcccggct acggtccgtc ccagaaggcc tacatgcgcg
     1321 acgtcggggc cgcccgggcc cttctcgccc aggcaggcta cgggccggcg catcccctcc
     1381 cgccgctcac gctctggaaa tcgagcagct cggtgagcgg gcgccaggcc gacacggtca
     1441 tggtgcgctc gctcgcggag gccgggatcc gggtgggcct ccgctacgag agctggggcg
     1501 tgctggacag catgatcacg ggccggaagg cggcgctctt cgcgctcgcg tgggtcgccg
     1561 acattcccga ccccgacacc ttcctccgcg ccctcggcta ctccacctcc tcgaccaact
     1621 acttcggctt ccacaaccgc ggcgtggact cgctgctgga cgtcgcgcgc aattccgccg
     1681 acccggacga gcggatggcc gcctaccgca aggccgagga gtcgatcgtg cgtgaggcgc
     1741 ccttcatccc gctctatcac accgcaagct tcatcgggat tcgcgacgac gtcctcgggc
     1801 tggagatgaa cccgctcggg atctcgacga tccagatgga gaagctccgg ttcaccgggc
     1861 ccgacgtggc cttcgacccg cgcagcgccg ctcgatgacc cgggtcgccg aggtggagcc
     1921 cgccgccgct cctcgccgcc cgcgcgccgg tctccaggcg cgcatcttcc tcacgttcgg
     1981 catcatcgtg gtcggcctgg tcgcggccgc ggtcaccttc ttcgtgcgga gcgagagcga
     2041 ctcgctcttg cgggagaccc ggaagcgcgg gttcgccgtg gcgcggagca tcgcctggct
     2101 gtccaccccc tcgctcctct cctacaacta catcgcgctg aaccaggccg cgcgccgctc
     2161 gcaggccggc ggcgagatcg cctacgtcgt catctacgac aaggaggggc agatcgcggc
     2221 cgacagccgc gtgcggaact cgttcggtca gccaccgcgc gacgcggacg actacgcggc
     2281 gcacgccgtg cgcgaggagc ggtggaagtt catcccgccc gcggccccgg gccgcccgcg
     2341 cgtgctggag atcctgcttc cggtctacgt ggacgggtac caggtgaagt ggggcacggt
     2401 gcgcgtcggc gtctcgctcg agagcatgga caaggagatc gccgcgctcg gacagcacct
     2461 cgtggtggcg ggcatcctcg cggccatcct ctgccttttc gcggcccgcg ccgcggcccg
     2521 cggcatcacg cgcccgatcg accgcctggt ctcggcgacg cgcgcggtcg cgaagggaga
     2581 ttactcccag cgcgtgaacc tccagacggg cgacgagctg gagaccctgg cctggcactt
     2641 cgaccggatg tcggacgagg tgcagcgcca gcaggcggag gtgctcgcga gccgcgagaa
     2701 cctcgcgcgg ctgaaccagt cgctggaggc cgcggtcgaa gcgcgcaccc acgcgctcgc
     2761 ggaatccgag g
//
LOCUS       DASSLZ010000322         5495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_628738, whole genome shotgun sequence.
ACCESSION   DASSLZ010000322 DASSLZ010000000
VERSION     DASSLZ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628738"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFM00_12170"
     CDS             complement(<1..485)
                     /locus_tag="VFM00_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S8"
                     /protein_id="HET7499047.1"
                     /translation="MSPHPRSFAAFVLLLCSFLYSTGALGAPPPRAIPNEVLIKFRPG
                     AAASDRAAIRADLHAQVLHDFAFIGVQHVKIRGVTVDAALSRYQHNPNVQYIEPNYEI
                     QALVTPNDPRFPEMYALRNTGQTGGTAGDDIKATLAWDQFTGDPNIKIGVIDTGVDYN
                     HP"
     gene            730..3369
                     /locus_tag="VFM00_12175"
     CDS             730..3369
                     /locus_tag="VFM00_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7499048.1"
                     /translation="MNLASGTRLGAYEIVGMIGSGGMGEVYRARDTRLDRTVAIKVLQ
                     GHLALSPETRQRFDREARIISSLNHPHICTLYDIGHQDGMDYLVMEYLEGESLADRLQ
                     RGPLPVPELVKIGMEIADALDRAHRQGLVHRDLKPGNIVITKGGAKLLDFGLARITGL
                     EEAPRSLSSPTMSRPLTAEGTIVGTFQYMAPEQLEGAETDARSDIFSFGSVLYEMASG
                     RRAFEGKSQASLIAAILREIPAPISSIRDVIPPAVERVILRCLEKNPDDRYQTARDVL
                     LDLKWAAEAGSRAGIPAPVAARRKHRERLAWSLAGAGLTAALLLGGFTVLHRPPEPVP
                     LRFFAPTPKGILLNDTPKVSPDGKYVAYNASDSTGRSCIWIRPLGSLTAEIVPGTDNA
                     RRPFWSPDSRYLGFMQDGKLRKVAAQGGPPQTICESQSAGDGTWSRQGVILFDGSPTD
                     SIRMVNAGGGSPRAATTIDHARGEVGSAWPHFLPDGRHFLYLGLGNRPDDTTLKVGDT
                     RSAKVVILAHGAFSRIEYVEPGYLLFARDRSLLAQPFDAGRLRLTGDAFPVVDDVAVG
                     GGVGSNADFSSSPTTLVFRGGAAGRRMHLVWVDRAGKMLQEFGQPGEYYNVALAPDGR
                     RVAVTYGQENTDIWIVDPARSVNTRFTFDAGGDFAPVWSPDGSKIAFMSDRTTPTGIY
                     IKQSSGAGAESLRFRLPVRSSVTGWSRDGRFLVGIARQPNTTWDIWIFPLDGSRQPYP
                     FVTGPTADVEPSISPDCRWIAYSSNESGRSEIYIQPLDGSGGKLQVSNQGGRDPHWRS
                     DGREMFYIAPGGDLTAVDVTLGATLALGTPHRLFRQALDQIDPNGHYFDVSADGQRLL
                     VRRDLDATDAPITTVFVNWMPKR"
     gene            complement(3443..3928)
                     /locus_tag="VFM00_12180"
     CDS             complement(3443..3928)
                     /locus_tag="VFM00_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499049.1"
                     /translation="MMPTFRPLTGLLTMIALFSVFAAPAHADVSGGLRAGFSSNPDQV
                     LVGGQLNMDPVSNHVYIVPSGEAGFGDDAFTLSFNGDVQYRFDVRDSKVRPYAGGGLT
                     LYYFKLSNDFGGGSDTNLGATVLGGIFFGREAGHPMFAEAKAGLTDKVPDWKFIFGIN
                     F"
     gene            complement(3978..4940)
                     /locus_tag="VFM00_12185"
     CDS             complement(3978..4940)
                     /locus_tag="VFM00_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET7499050.1"
                     /translation="MFRRVLVANRGEIAVRVIRALHELGIEAVAIYSTADSDALHVRM
                     ADHAVRVGPPTASESYLRIPSVIAAAETTGCEAVHPGYGFLAENPAFVEACVDNDLVF
                     IGPPADVMALMGDKISAKQAMRAADVPTVPGTEGATSLVDVRGVASDIGFPLLLKATA
                     GGGGKGMRLVTEPGDLEDAYRTASTEAEAAFGDGTMYVEKALVPARHVEILAYLRLGR
                     PREALSDLAQAESLMPGETASLHFPRGNAYMSLGDDAHAVEEFGRAIEAREGGADAYV
                     NRGLARMRLGDRSGAGSDFREALRLDPNHAEAQAQLLRLAPKAP"
     gene            complement(4940..>5495)
                     /gene="accB"
                     /locus_tag="VFM00_12190"
     CDS             complement(4940..>5495)
                     /gene="accB"
                     /locus_tag="VFM00_12190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET7499051.1"
                     /translation="LLLLALFGEEAEALLRSIRERSGGEESLAASGVDRARAEKIRDL
                     VRIVQETGIGEITIEEDGMRVSVRRQAEQGAVPVVAAPQPPPDAEQGVATPRDDALVR
                     VESPMVGTFYRAPSPDAAPFVQEGDAVAPGQTLCILEAMKLMNEVKAEIEAVVRAIHV
                     GNAEPVEYGQLLFELEPVAGPPLL"
BASE COUNT          870 a         1852 c         1880 g          893 t
ORIGIN      
        1 gggtggttgt agtccacgcc cgtgtcgatg acgccgatct tgatgttcgg atcgccggtg
       61 aactgatccc aggcgagcgt cgccttgatg tcgtcgcccg ccgtccctcc ggtctgcccc
      121 gtgttccgca aggcgtacat ctcggggaag cgcggatcgt tgggggtcac cagcgcctgg
      181 atctcgtagt tgggctcgat gtactgcacg ttcgggttgt gctggtagcg cgagagtgcc
      241 gcgtccaccg tcacaccgcg gatcttcacg tgctggactc cgatgaacgc gaagtcgtga
      301 aggacctggg cgtgcagatc ggcgcggatg gctgcgcgat cggaagccgc ggcgccggga
      361 cggaacttga tgaggacttc gttgggaatg gcgcggggcg gtggggcgcc gagcgcgccg
      421 gtcgaataga ggaaggagca aagcaagagc acgaaagccg caaaggatcg cggatgcggg
      481 gacatgccag acctcctgga agaatctcgt gccgcggcct cgagcgcggc aagggcatgg
      541 ttcgcgatgc gggtgtgctg ctcgtggatg gtgcggaacg gggtttcctg gggccggtcc
      601 acctcctgcg ccggagacga gagccgaaac agcagcgtgt tttcgaagga ggcgggatta
      661 tatcgcgaaa gcgactcagg gtcaacggat tccccgtttt gcgggcgtcg ttgtggtaat
      721 tttccgccca tgaacctcgc ctcgggtacc cgtctcggcg cctacgagat cgtcggcatg
      781 atcggcagtg gcggcatggg cgaggtctac cgcgcccgcg atacgcggct ggaccggacg
      841 gtggcgatca aggtgctgca ggggcacctc gcgctcagcc ccgagacacg gcagcgcttc
      901 gatcgcgaag cgcgcatcat ctccagcctc aaccatcccc acatctgcac gctgtacgac
      961 atcgggcacc aggacgggat ggactatctc gtcatggaat atctcgaagg ggagtcgctc
     1021 gccgaccgcc tgcagcgcgg accgctgccc gttcccgagc tcgtgaagat cggcatggag
     1081 atcgccgacg cgctcgaccg cgcgcaccgc cagggcctgg tccatcggga tctgaaaccc
     1141 gggaacatcg tcatcaccaa gggcggggcc aagcttctcg acttcggact cgcgcggatc
     1201 accgggctgg aggaggcgcc gcgcagcctc tcctccccga cgatgagccg cccgctcacc
     1261 gcggagggga cgatcgtcgg cacgttccag tacatggctc ccgagcagct cgagggggcg
     1321 gagaccgacg cgcggagcga catcttctcg ttcggctcgg tgctctacga gatggcgtcg
     1381 gggcggcgcg cattcgaggg gaagagccag gccagcctga tcgccgcgat cctgcgcgag
     1441 atccccgcgc cgatctcgtc gatccgcgac gtgatcccgc ccgcggtgga gcgcgtcatc
     1501 ctgcgctgcc tggagaagaa ccccgacgat cgctaccaga ccgcgcgcga cgtgctgctc
     1561 gacctcaagt gggctgccga agccggctcc cgcgcgggca ttcccgcgcc ggtcgcggca
     1621 cggcgcaagc atcgagagcg cctggcctgg tccctcgcgg gcgcggggct gaccgccgcg
     1681 ctcctcctgg gcggtttcac tgtcctgcat cgcccgcccg agccggtgcc gctccgcttc
     1741 ttcgcgccga cgcccaaggg catcctgctc aacgacacgc ccaaggtctc gccggacggg
     1801 aagtacgtcg cctacaacgc ctcggattcg acgggaaggt cctgcatctg gatccgcccg
     1861 ctcgggtcgc tgacggcgga gatcgtcccg ggaacggata acgcgcggag gcccttctgg
     1921 tcgccggaca gccgctatct cgggttcatg caggatggga agctgcgcaa ggtcgccgca
     1981 cagggaggac cgccgcagac gatctgcgaa tcgcaaagcg ccggcgacgg cacctggagc
     2041 cggcagggcg tcatcctgtt cgacggaagc ccgaccgact ccatccggat ggtcaacgcc
     2101 ggcggcggat cgccgcgggc cgccacgacg atcgaccacg cgcgtggcga agtcgggagc
     2161 gcatggcccc atttcctgcc ggacgggcgg catttcctgt atctgggatt ggggaaccgc
     2221 cccgacgaca cgacgctgaa ggtcggggat acccggtccg cgaaggtggt catccttgct
     2281 cacggcgcct tctcccggat cgagtacgtc gagcccgggt atctgctgtt cgcccgggac
     2341 cgctcgctgc tggcccagcc cttcgacgcg ggccggctgc gcctcacggg ggatgcgttt
     2401 ccggtcgtgg acgacgtggc cgtcgggggt ggggtcggct ccaacgccga cttctcgtcc
     2461 tcgccgacga cgctggtctt tcgcggaggc gccgcgggac gccgcatgca tctggtgtgg
     2521 gtggatcgcg cggggaagat gcttcaggaa ttcgggcagc ccggcgagta ttacaacgtg
     2581 gcgctcgcgc ccgacggacg gcgggtcgcg gtcacctatg gccaggagaa cacggacatc
     2641 tggatcgtcg acccggcccg aagcgtcaat acacgcttta ccttcgatgc cggcggcgac
     2701 ttcgcgccgg tctggtctcc cgatggatcg aaaatcgctt tcatgtcgga tcgcacgacg
     2761 ccgacgggga tctatatcaa gcagtcgtcc ggcgccggcg ccgaatccct tcgattccgt
     2821 ctaccggtcc ggtcttcggt caccggctgg tcgcgtgacg ggcggttcct ggtgggcatc
     2881 gcgcgccagc cgaacacgac gtgggacatc tggatcttcc ctctcgatgg ctcccgacag
     2941 ccctatccgt tcgtgaccgg tcccaccgcg gacgtggagc cgtcgatttc tcccgactgt
     3001 cgatggattg cgtactcatc caatgagtcc gggcggagcg agatctacat ccaacctctg
     3061 gatggctcgg gcgggaagct ccaggtttcc aaccagggcg gccgcgaccc ccactggcgc
     3121 tccgatggac gggagatgtt ctacatcgct ccgggagggg acctcactgc ggtcgatgtg
     3181 accctgggcg cgaccctcgc gctcgggacg ccgcaccggc tgttccggca ggcgctggac
     3241 cagatcgacc cgaacgggca ctacttcgac gtctccgcgg acggacagcg attgctggtt
     3301 cgccgtgatc tggatgcgac ggatgccccg atcacgacgg tcttcgtgaa ctggatgccg
     3361 aaacgctaag ccgcgatgga cgccggacgc ccatcgcggc ctggttgacc gcaacacgcc
     3421 tcgaagggca aatcctctcg ccctagaagt tgatcccgaa gatgaacttc cagtccggca
     3481 ctttgtcggt gagtcccgcc ttggcctcgg cgaacatcgg gtggccggcc tccctgccga
     3541 aaaagatgcc ccccaggacc gtggcgccga gattggtgtc gctcccgccg ccgaaatcgt
     3601 tgctcagctt gaagtagtag agcgtcaggc cgccgccggc ataagggcgc actttggagt
     3661 cgcgcacgtc gaagcggtac tggacgtcgc cgttgaagga gagggtgaac gcgtcgtcgc
     3721 cgaaccccgc ctcgccgctc gggacgatgt agacgtggtt gctcaccgga tccatgttga
     3781 gctgcccgcc taccagcacc tggtccgggt tgctgctgaa tccggcgcgg agacctcccg
     3841 aaacgtcggc gtgagccgga gcggcgaaga cggagaacag cgcgatcatc gtcagaagac
     3901 cggtcagcgg tcggaacgtc ggcatcatga cctcctcatg ggtgggcatc cgaacgagac
     3961 agacgaagta cagcgcctca cggcgctttc ggcgccagcc gcagcagctg ggcctgggct
     4021 tcggcgtggt tgggatcgag ccgaagggct tcacgaaaat cggatccggc gccgctccgg
     4081 tctccgaggc gcatgcgggc caggccccgg ttgacgtagg cgtcggcacc tccctcgcgc
     4141 gcctcgatgg cgcgcccgaa ctcctcgacc gcgtgcgcgt cgtcgccaag gctcatgtag
     4201 gcgttgccgc gcggaaagtg cagggatgcg gtttcgccgg gcatgagcga ttccgcctgc
     4261 gcaagatcgg acaacgcttc ccggggcctg cccagacgca ggtacgcaag gatctccacg
     4321 tggcgcgccg ggacgagcgc cttctcgacg tacatcgtgc cgtcgccgaa cgccgcctct
     4381 gcttcggtcg acgcggtgcg gtacgcgtcc tcgaggtctc cgggctcggt gacgagccgc
     4441 atgcctttac cgcctccgcc cgccgtcgcc ttgagcagga gcggaaagcc gatgtcggag
     4501 gcgacgccgc gcacgtcgac gagcgaggtc gcaccctccg tgcccggcac cgtcggcaca
     4561 tcggcggcgc gcatcgcctg tttcgccgag atcttgtcgc ccatgagtgc catcacatcc
     4621 gcgggcgggc cgatgaagac gaggtcgttg tcgacgcacg cctcgacgaa cgccgggttc
     4681 tccgcgagga agccgtagcc gggatgcacc gcttcgcagc cggtcgtctc ggccgccgcg
     4741 atgacgctcg ggatgcggag gtagctctcc gaggccgtcg gcggccctac gcgcaccgca
     4801 tggtcggcca tgcgcacgtg gagtgcgtcg ctgtccgcgg tcgagtagat ggcgaccgcc
     4861 tcgatgccga gctcgtgcag cgcgcggatg acgcgaaccg cgatctcgcc ccggttcgcg
     4921 acgaggaccc ggcgaaacat cagagaaggg gcggtccggc gaccggctcg agctcgaaca
     4981 gcagctggcc gtactcgacc ggctcggcat tcccgacgtg gatggcgcgg acgaccgcct
     5041 cgatctccgc cttcacctcg ttcatcagct tcatcgcctc gaggatgcag agcgtctgcc
     5101 ccggggcgac cgcgtcgccc tcctgcacga acggtgccgc gtccggcgaa ggtgcgcggt
     5161 agaacgtccc gaccatcggg ctctcgacgc ggacgagcgc gtcgtcgcgc ggcgtggcca
     5221 cgccctgctc cgcgtcgggc ggcggctgcg gcgccgcgac gaccggcacg gccccctgct
     5281 cggcctggcg gcggacgctg acgcgcatcc cgtcctcttc gatcgtgatc tcgccgatgc
     5341 cggtctcctg gacgatccgc accaggtcgc ggatcttctc ggcgcgagcc ctgtccactc
     5401 cactggcggc gagcgactcc tcgccgcccg agcgctcgcg gatcgagcga aggagcgctt
     5461 cggcctcctc gccgaacagc gccaacagga gaagc
//
LOCUS       DASSLZ010000323         4180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1640662, whole genome shotgun sequence.
ACCESSION   DASSLZ010000323 DASSLZ010000000
VERSION     DASSLZ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640662"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /gene="eno"
                     /locus_tag="VFM00_12195"
     CDS             <1..1326
                     /gene="eno"
                     /locus_tag="VFM00_12195"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET7499052.1"
                     /translation="REILDSRGQPTVEVEVALSGGAIGRAAVPSGASTGSREALELRD
                     GDAKRYLGKGVSRAVLNVNETIAARIVGEDASDQAGVDGILIELDGTPSKKNLGANAV
                     LGVSLACAKAAADAHLLPLYRYLGGAGARTLPVPLMNVINGGAHADNNLDIQEFMIVP
                     LGAPSFREALRYGAEVFHTLKRLLKDKGLVTAVGDEGGFAPNLKSNAEALDLLVGAIE
                     KAGYRPGADVALALDVAASELYEGGAYALDGRRGIDSAGMIAYLKGLADRYPIVSIED
                     GLAEGDWDGWRRLTEELGDAIQIVGDDIFVTNPAILRRGIDEGAANSILVKLNQIGTV
                     TETLDAIGMATRAGFTTVISHRSGETEDSSIADLAVAVNAGQIKTGSLSRSDRIAKYN
                     QLLRIEEQLADAALYPGAGAFRGSAAARLKGSGASRPGANAAAGAAARV"
     gene            1330..1713
                     /locus_tag="VFM00_12200"
     CDS             1330..1713
                     /locus_tag="VFM00_12200"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET7499053.1"
                     /translation="MLRSPYQSPDGPPRERAKRFLRLPPKETRQKHRGLWILGIALLA
                     WLLWSFVGSDTGVFRTAALQRETAALEKRKLELTARAEALEARRKEQAKDPLLEERVA
                     RERFHLVKKDEIVYRYQDDGADSGR"
     gene            complement(1683..4010)
                     /locus_tag="VFM00_12205"
     CDS             complement(1683..4010)
                     /locus_tag="VFM00_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7499054.1"
                     /translation="MESSERSGDVVIGRTLQRYKILEPLGSGGVGEVYRARDERLGRE
                     VALKILRSDAADGASRDRLRREARTLSQLNHPGISTIHDVETADGVDFIVMELVTGET
                     LASRLARGALSEGIARVYGAEIADALGAAHDRGVIHRDLKPGNVMIDERGRLKLLDFG
                     LALYRPASVSSADSTADALSGALVGTLPYMAPEQLLGRALDPRADLYSLGVLLYEMTT
                     GRRPFQAEPATALINEILNAPAPPPSAWAPHLSEECSRLIQALLEKDPARRPASATDV
                     AAALRESGRASDGSSVAVGAAAVAGAAAIASVAVLPLANLTGAPDQEFFVDGMTEALI
                     ATLAQVRALRVVSRTSVMRYKGTAEPLPAIARALEVDAIVEGAVARSADRVRVTAQLI
                     DATSDRHLWARSYERDVHDVLALQSELAQAIAEEIQVHVTHEERARLVNPKRVDPGAY
                     EEYLRGRFYWNRRNEAAARRAIECFERSIARDPAWAPAHSGIADAWLTLANYDYVPPM
                     EAYPRAEAAVERALALDDQLAEAYVSLGALRTDAHWDWPGAEAAFRRALALDPNNAAA
                     YHWYGDLLSLTSRHDEALAAARRARELDPLSLVVSTSLGIHLFYARRYDEAVAQQQRT
                     LELDPTFAPAIRNLGGAYEQLGRYDDAIAAYHRAHALLPSELSATTLLGHALAIAGRT
                     EEARKLLSELTEESGRRYVSRYRLAAIHLALGERGIALDFLEQALRGRDRAMIWLNVA
                     PRFDALRGEPRFQAIVRTMRLDPAAGQRPESAPSS"
     gene            4099..4175
                     /locus_tag="VFM00_12210"
     tRNA            4099..4175
                     /locus_tag="VFM00_12210"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4133..4135,aa:Met,seq:cat)
BASE COUNT          611 a         1498 c         1505 g          566 t
ORIGIN      
        1 cgcgaaatcc tggattcccg cggccagccg accgtggagg tcgaggtcgc cctctccggg
       61 ggcgcgatcg gacgcgcggc cgtgccctcc ggcgcttcca ccggctcccg ggaagcgctg
      121 gagctgcgcg acggggacgc caagcggtac ctgggcaaag gggtttcccg cgccgtcttg
      181 aacgtgaacg agacgatcgc ggcgcggatc gtgggcgagg acgcctccga ccaggccggg
      241 gtggacggga tcctgatcga gctggacggc accccttcga agaagaacct cggcgccaac
      301 gcggtgctgg gggtatcgct cgcctgcgcc aaggcggcgg ccgacgcgca cctgcttccc
      361 ctctaccgct acctgggcgg agccggggcg cgcacgctgc ccgttccgct catgaacgtg
      421 atcaacggcg gcgcgcacgc ggacaacaac ctcgacatcc aggagttcat gatcgtcccg
      481 ctcggcgccc cctcgttccg cgaggcgctc cgctacggcg cggaggtctt ccacacgctg
      541 aagcgcctcc tcaaggacaa gggcctcgtg accgccgtcg gcgacgaagg cggcttcgcg
      601 ccgaatctca agagcaacgc cgaggcgctc gacctgctcg tcggcgccat cgagaaggcg
      661 ggataccgcc ccggcgcgga cgtcgcgctg gccctggacg tcgcggcctc cgagctgtac
      721 gagggcggcg cttatgcgct cgacggccgg cgcggcatcg acagcgcggg aatgatcgcg
      781 tacctcaagg ggctcgccga ccgctacccg atcgtctcca tcgaggacgg cctcgccgag
      841 ggggactggg acggctggcg gcggctcacc gaggagctgg gcgacgccat ccagatcgtc
      901 ggcgacgaca tcttcgtcac caacccggcg atcctccggc gcgggatcga cgagggcgcc
      961 gcgaactcga tcctggtcaa gctgaaccag attggcaccg tgaccgagac cctggacgcg
     1021 atcggcatgg cgacccgggc gggcttcacc acggtgatct cgcaccgctc gggcgagacc
     1081 gaggattcct cgatcgccga tctcgccgtg gcggtgaacg cggggcagat caagaccggc
     1141 tccctctcgc gcagcgaccg gatcgcgaag tacaaccagc tcctccgcat cgaagagcag
     1201 ctggccgacg cggcgctcta tccgggcgcg ggcgccttcc gcggctcggc cgccgcgcgg
     1261 ctcaaggggt ccggcgcatc ccgccccggc gcgaacgcgg cggcgggcgc cgcggcgcgg
     1321 gtctagggcg tgctccgctc cccctaccag agtcccgacg gaccgccgcg cgagcgcgcc
     1381 aagcggttcc tgcggcttcc gcccaaggag acgcgccaga agcaccgcgg cctctggatc
     1441 ctgggaattg ccctgctcgc ctggctcctc tggtcgttcg tgggaagcga caccggcgtg
     1501 ttccgcaccg cggcgctcca gcgcgagacc gccgcgctcg agaagcggaa gctggagctg
     1561 accgcgcgcg ccgaggcgct cgaagcccgg cgtaaggagc aggccaagga tccgctcctc
     1621 gaggagcggg tggcccgcga gcgtttccac ctcgtcaaga aggacgagat cgtctaccgc
     1681 tatcaggacg acggagccga ctccgggcgc tgacccgccg ccgggtccag gcgcatcgtc
     1741 ctcacgatcg cctggaagcg cggctcgccg cgcaaggcgt cgaaccgcgg cgccacgttg
     1801 agccagatca tcgcccggtc gcggccgcgg agcgcctgct ccaggaagtc gagcgcgatc
     1861 ccccgctccc ccagcgccag gtggatcgcc gccagccggt accgcgacac gtagcgcctc
     1921 cccgattcct ccgtcagctc gctcaacagc ttccgcgcct cctccgtccg ccccgcgatc
     1981 gcgagcgcgt gcccgagcag cgtggtggcc gagagctccg aggggaggag cgcgtgcgcg
     2041 cgatggtagg ccgcgatggc gtcgtcgtag cggcccagct gctcgtacgc cccgcccagg
     2101 ttccggatcg ccggcgcgaa cgtgggatcg agctcgaggg tgcgctgctg ctgcgccacg
     2161 gcttcgtcgt agcgccgcgc gtagaagagg tggatcccga gtgacgtgct gaccacgagc
     2221 gaaagcgggt ccagctcgcg ggcgcggcgg gcggcggcga gcgcctcgtc gtggcgcgag
     2281 gtgagggaaa gcaggtcgcc gtaccagtgg taggcggccg cgttgttcgg atcgagcgcc
     2341 agcgcgcgcc ggaacgcggc ctcggccccc ggccagtccc agtgcgcgtc ggtgcggagc
     2401 gcgcccagcg agacgtacgc ctcggccagc tggtcgtcca gcgccaacgc gcgctcgacg
     2461 gccgcctcgg cgcgcggata cgcctccatc ggcgggacgt agtcgtagtt cgccagcgtg
     2521 agccacgcgt cggcgatccc cgagtgggcc ggcgcccacg ccgggtcgcg cgcgatcgac
     2581 cgctcgaagc attcgatggc gcgccgcgcc gccgcttcgt tccgccggtt ccagtagaag
     2641 cgcccgcgca gatattcctc gtaggcgccg ggatccacgc gcttggggtt cacgagccgc
     2701 gcgcgctcct cgtgcgtcac gtgcacctgg atctcctcgg cgatcgcctg cgccagctcc
     2761 gactggagcg ccagcacgtc gtggacgtcg cgctcgtagc tgcgcgccca gagatgccgg
     2821 tcgctcgtgg cgtcgatgag ctgcgccgtg acgcgcacgc ggtccgccga ccgcgccacc
     2881 gcgccctcga cgatcgcgtc cacctcgagc gcgcgggcga tcgcgggcag gggctccgcg
     2941 gttcccttgt agcgcatgac cgacgtgcgc gagacgacgc gcagcgcgcg cacctgggcg
     3001 agcgtggcga tgagcgcctc ggtcatccca tccacgaaga actcctggtc gggtgcgccg
     3061 gtcaggttgg cgagcggaag caccgcgacc gaggcgatcg cggccgcgcc ggccaccgcg
     3121 gccgcgccca ccgcgacgct cgagccgtcg gacgcacgcc ccgattcacg cagcgccgcg
     3181 gccacgtccg tggccgaggc gggacgccgt gcgggatcct tttccaggag cgcctggatg
     3241 agacgcgagc actcctcgga gagatggggc gcccacgccg agggcggcgg ggcgggcgcg
     3301 ttcaggattt cattgatgag cgccgtcgcg ggctcggcct gaaagggacg ccgtccggtc
     3361 gtcatctcgt aaagcagcac gcccagcgag tagagatcgg cgcgaggatc gagcgcgcgg
     3421 cccaggagct gctcgggcgc catgtagggg agcgtcccga ccagggcgcc gctcagggcg
     3481 tccgcggtcg agtcggcgct ggagaccgag gcgggacggt agagcgccag cccgaaatcg
     3541 aggagcttca gccggccgcg ctcgtcgatc atcacgttcc ccggcttcag atcccggtgg
     3601 atgacgccgc gatcgtgcgc cgcgcccagc gcgtcggcga tctcggcgcc gtacacgcgc
     3661 gcgatcccct cggagagcgc cccgcgcgcc aagcgggagg cgagcgtttc ccccgtgacc
     3721 agctccatca cgatgaagtc gacgccgtcg gcggtctcga cgtcgtggat ggtgctgatg
     3781 ccggggtggt tgagctgcga cagggtccgg gcttcccgcc tcaggcggtc gcgggacgcc
     3841 ccgtcggcgg cgtcggagcg gaggatcttg agcgcgacct cgcggcccag gcgctcgtcg
     3901 cgggcgcgat agacctcgcc aacgccgccc gagccgagcg gctcgaggat cttgtagcgc
     3961 tgaagggttc ggccgatgac gacatcgcct gagcgttccg atgactccac gacacccccg
     4021 gggtttccgt cttggatgcg cggtcgaggc tggaaggaaa gcactttaca cgagggcggc
     4081 gttcggggag tattcccctg cggggtggag cagcctggta gctcgtcggg ctcataaccc
     4141 gaaggtcaga ggttcaaatc ctctccccgc aaccactttg
//
LOCUS       DASSLZ010000324         6874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1860821, whole genome shotgun sequence.
ACCESSION   DASSLZ010000324 DASSLZ010000000
VERSION     DASSLZ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860821"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VFM00_12215"
     CDS             <1..578
                     /locus_tag="VFM00_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499055.1"
                     /translation="PAMGVIPNSSVTYARQAADFDLYRLHGTWYLVDGGVWYRSTSWR
                     GPFLSIRASAVPREVLTIPSRYRRDWTVSSADDNDRDRYGYRDRDGYRDNDRDRDRDR
                     YQTIRYWNSGRTFTVEPSGDMIPGTDIDYVRGNASYDLYRYNDSWYLADQTGWYRADT
                     WRGPFISIQLGSVPRAVISVPMTYRRYWSTD"
     gene            724..1581
                     /locus_tag="VFM00_12220"
     CDS             724..1581
                     /locus_tag="VFM00_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499056.1"
                     /translation="MSRSPASPLVVLPSCLFLLSLVSTAALAEESPPWLSDRGPGIAT
                     SLFGTYVQKGELLLYPFYEYTWNRDQEYKPEELGYAGSHDQRGTLIEHEGLIFLAYGV
                     SRRVALELESALIADATLHKDPSDLSGVPRRLHESGFGDTQAEIRWRWVEETASRPEL
                     WSYFEAVFPFQKNRKLIGTQDWELVPGVGATKGFGWGTVTTRISGTYDAEGGDFVFGE
                     YALEYLKRLTPRWRLVLALEGEQDEAAVIGELQWRFAPRALLKLNNGFGVTSKAPDIA
                     PEVGVALSF"
     gene            complement(1619..3871)
                     /gene="ccsA"
                     /locus_tag="VFM00_12225"
     CDS             complement(1619..3871)
                     /gene="ccsA"
                     /locus_tag="VFM00_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET7499057.1"
                     /translation="MNATLRTLASLELSIILLVSIAVVMSFGTIIESLQGAEAARFVY
                     HAFWFRLLLGLFALNVGAALWERWPRNRWRIGFLVTHASLLLILVGSLVTAVAVQEGR
                     LPLWEGESASHFFSEAPGQADVHRNLPFAVRLDDFQIDVYPGTQRPAMFRSRVTVRDP
                     SGAETPAVIQMNRPLSHGGYRFFQSSYQIQGGREMSILSVSKDPGQPIVFWGYGLLVL
                     GMLVVLSTRIAQRRSGMMGVAVLAGLAACAGTSQAAGAPSQDLLDRIHRLPVQSDGRV
                     MPLDTQARDDVRTVTGRGGWKGQDPVAVVLGWTMDPERWSDEPVVLLDGDVAARIGRA
                     SANRYASYHSLVSSEPLHQAVQAAMARQEGGGKPSKADKHLLRVSERLETLHGYLTGQ
                     AIRAIPAADPVAAWTPPSGLKTLEAWAGLEAQVRPTAPSHYPTPPAIEREIRYNAVHP
                     VRVAWILLLPAAIGAGLTLEKNRWRLRWTTGLLVVLGFAVMTWGIATRWQIAGHIPAS
                     NMYESMLFLGWGVGLFGVVSVLARNRMLIYNAAAMAALVMLLLDRLPIDPFIHPIPPV
                     LSGTPWLAIHVPIIMVSYSVFAIATFLAHLVLGAAIFAPRRRGLADRWSDLLYWYLVV
                     GSIFLIAGILTGSIWAASSWGRYWGWDPKEVWSLVAFLAYMAILHARFDGRLGPFGVA
                     AWSIGAFWTILMTYLGVNFVLASGLHSYGFGSSGLVQTMVVIALVEVAFLFAGWRAHR
                     NLGAAVSRRG"
     gene            complement(3983..4489)
                     /locus_tag="VFM00_12230"
     CDS             complement(3983..4489)
                     /locus_tag="VFM00_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7499058.1"
                     /translation="MKTTEWLLAQLESEAPRTRRALEHVPEGRDDWKPHDKSMPLGRL
                     AMLVATIPTWVNLIVNQDELDVAPKAGHPDRKPLRTRRELIEAHDKAVEEARRTLRAA
                     SDEHLKTPWRLLAGGEVKSEEPREVVLRDTMMHMAHHRGQLTVYLRLNDALVPSIYGP
                     TADDKQFA"
     gene            4776..5642
                     /locus_tag="VFM00_12235"
     CDS             4776..5642
                     /locus_tag="VFM00_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499059.1"
                     /translation="MIRKSVAIVLAVVCLALLGAAGVTYSKYKKSQAGLAQASADQEA
                     MRQRYDQAVNEIVSIQDSLNAIAIGENHAQGDIELHGQTIHEKVLDRITTIKASIART
                     KARIDELDARLKHNGIRMASMKRMIEGLKESVQYREDKIAMLSTQVDTLRTRVTGLKF
                     EIQGQQEALQRNQDELLARQKELATIYYTMGTKDELKNAGVVQPNGGVLGVGKTLKVS
                     GRFEEAAFSPLNTDEQNVIQIPAKKGKVQILSAQPPGSYSLTKVGKDMVELHILDRTE
                     FRKIKHLVILKA"
     gene            complement(5750..6223)
                     /locus_tag="VFM00_12240"
     CDS             complement(5750..6223)
                     /locus_tag="VFM00_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499060.1"
                     /translation="MTDPAVTMRQLRAYALSATAEDLDLEPTEARPRVWGAIMELGYA
                     TGIATLLSLADGTTSLYFSNGGGVIGAGENAAVREAAEAFLDAAETHAPALPSVEDTP
                     TPRIGRVRLYVRTFEGTLGIEATDEELSQNRHPLSAVFHAGHAVITAIRENSSEA"
     gene            6334..>6874
                     /locus_tag="VFM00_12245"
     CDS             6334..>6874
                     /locus_tag="VFM00_12245"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7499061.1"
                     /translation="MPPARFCTECGAALTAAGATRSSPDSHPSSQSSSSIHGRFEPGT
                     RLGTRYRVVGLLGRGGMGEVYRADDLELNQSVALKFLPEKVARNPSDLARLRQEVRIA
                     RQIAHPNVCRTYDISEAGGQVFVVMEYVDGEDLASVLRRLGRPTPDKALEIARQLCLG
                     LGAAHENGVLHRDLKPANIM"
BASE COUNT         1159 a         2306 c         2347 g         1062 t
ORIGIN      
        1 agccggcgat gggcgtcatt cccaacagct cggtcacgta cgcgcgccag gccgccgact
       61 tcgacctcta tcgcctgcac ggcacctggt acctggtgga cggcggtgtt tggtatcgct
      121 ccacgtcgtg gcgcgggccg ttcctctcga tccgggcatc cgccgttccg cgtgaggtcc
      181 tcaccattcc ctcgcgctat cgccgcgact ggaccgtctc cagcgccgac gacaacgatc
      241 gcgaccgcta cggctaccgc gaccgcgacg gctaccgcga caacgaccgc gaccgcgaca
      301 gggatcgcta tcagacgatc cgctactgga actcggggcg gaccttcacg gtcgagccga
      361 gcggggacat gatcccgggc accgacatcg actacgtccg cggcaacgcg agctacgacc
      421 tgtaccgcta caacgactcg tggtatctcg ccgatcagac cggctggtac cgcgccgaca
      481 cgtggcgcgg gccgttcatc tcgatccagc tgggcagcgt gcctcgggcc gtcatctcgg
      541 ttccgatgac gtatcgccgc tactggagca ccgactaacg ctccgatcgg atacgggcgt
      601 ttcgcggaag tcccgggcgc ctcggcgtcc gggacttctc cgttcttcgg agcgcatggg
      661 gcggtgtggg gtaccactgc gaggcacgtt cggacgcaat accccctccc cggaggctgg
      721 tccatgtccc gctcgccggc ttcgcctctc gtcgtcctgc cctcctgcct cttcctcctg
      781 tctctggtat cgaccgccgc gttggccgag gagtcgccgc catggctctc cgatcgcgga
      841 ccgggcatcg cgacgtccct cttcggcacg tacgtacaga aaggcgagct gctcctctat
      901 cccttctacg agtacacatg gaatcgggat caggagtaca agccggagga gctgggatac
      961 gccggctcgc acgatcagcg cggcacgttg atcgagcacg aggggctgat cttcctggcg
     1021 tacggcgtct cgcgccgcgt ggccctcgag ctcgagtcgg cgctgatcgc cgacgccacg
     1081 ctgcacaagg acccctccga tctctccggc gttccgcggc gcctgcacga gtccggcttc
     1141 ggcgatacgc aggccgagat ccggtggcgc tgggtggagg agacggcgtc ccgaccggag
     1201 ctctggagct acttcgaggc ggtgtttccc tttcagaaga accggaagct cattggcacg
     1261 caggactggg agctggtccc gggtgtcggc gccacgaagg ggtttggatg gggaacggtc
     1321 accaccagaa tctcagggac ctacgacgcc gaaggaggcg acttcgtgtt cggggaatac
     1381 gctctcgaat acctgaagcg cctgaccccg cgctggcggc tggttctggc gctcgagggg
     1441 gaacaggacg aggccgccgt gatcggggag ctccagtggc gattcgcgcc ccgggcgctc
     1501 ctcaagctga acaacggctt cggcgttacc agcaaggctc cggacatcgc gcccgaagtc
     1561 ggagtggcgc tctcgttcta ggcgcaggcg ctcggtcgca ggcccgaaga ccgccgggtc
     1621 atccgcgacg cgatacggcc gctcccaggt ttcgatgcgc gcgccacccc gcgaagagga
     1681 acgcgacctc gacgagggcg atgaccacca tcgtctgaac cagtcccgag ctgccgaacc
     1741 cgtaggaatg cagtcccgac gccaggacga agttcacgcc gagataggtc atgagaatcg
     1801 tccagaaggc gccgatcgac caagccgcga cgccgaaggg tccgagccga ccgtcgaagc
     1861 gcgcgtgcag gatcgccatg taggccagga acgcgacgag cgaccagacc tccttgggat
     1921 cccatcccca gtaccgtccc cacgacgagg cggcccagat cgatccggtg aggattcccg
     1981 cgatcaggaa gatcgagcct acgacgaggt accagtacag caggtcgctc cagcggtcgg
     2041 cgaggccccg ccgccgcggc gcgaagatgg cggcccccag caccaggtgc gcgaggaacg
     2101 tggcgatcgc gaagacggag tagctcacca tgatgatcgg cacgtggatc gcgagccacg
     2161 gcgtccccga tagcaccggc gggatcggat ggatgaaggg atcgatcggg agccggtcca
     2221 gcagcagcat caccagcgcc gccatggccg cggcgttgta gatcagcatg cggttccgcg
     2281 ccagcaccga cacgacgccg aacaggccga cgccccatcc caggaagagc atcgactcgt
     2341 acatgttcga agcggggatg tggcccgcga tctgccagcg cgtcgcgatg ccccaggtca
     2401 tgacggcgaa ccccagcacg accaggagtc ccgtggtcca acggagccgc caccgattct
     2461 tctccagcgt gagacccgcg ccgatcgccg cggggaggag aaggatccag gccacgcgga
     2521 ccggatgaac ggcgttgtag cggatctccc gctcgatcgc cggaggcgtg gggtagtggg
     2581 agggagccgt ggggcgcacc tgcgcctcca gaccggccca ggcctccagg gtcttcaggc
     2641 cgctcggggg agtccaggcg gccaccggat ccgcggccgg aatcgcgcgg atcgcctgcc
     2701 cggtcaggta gccgtggagc gtttcgagac gctcgctcac gcggagcagg tgcttgtcgg
     2761 ccttggaggg cttgccgccc ccttcctgac gcgccatggc ggcctgcacc gcctggtgga
     2821 gcggctcgct cgagaccagg ctgtgatagc tcgcgtaccg attcgccgag gcacggccga
     2881 tccgggccgc gacgtcgccg tccaggagca cgaccggctc gtccgaccac cgctccgggt
     2941 ccatggtcca tccgaggacc acggcgaccg ggtcctgccc cttccatcca ccgcgtcccg
     3001 tgacggttcg cacgtcgtcg cgagcctggg tgtccagcgg catcacccgt ccgtcgctct
     3061 gcaccggcag ccgatggatc ctgtcgagca ggtcctgcga aggcgcgccg gcggcttgag
     3121 aggtgccggc gcatgcggcg aggcccgcga ggacggcgac ccccatcatg ccgctccgcc
     3181 gctgggcgat ccgcgtcgac agcacgacga gcatgcccag gacgaggagg ccgtagcccc
     3241 agaagacgat cggctgtcca gggtccttgg acaccgagag gatcgacatt tcgcgcccgc
     3301 cctggatctg atagctcgac tggaagaagc ggtagccgcc gtggctgagc gggcggttca
     3361 tctggatcac cgcaggagtc tccgcgccgg aaggatcgcg caccgtgacg cggctccgga
     3421 acatggccgg tcgctgcgtg ccggggtaga cgtcgatctg gaagtcgtcg agccggaccg
     3481 cgaaggggag gttccggtgc acgtcggcct ggccgggagc ctcggagaag aagtggctgg
     3541 cgctctcgcc ctcccagaga ggaaggcgtc cttcctggac cgccacggcc gtcaccagcg
     3601 agccgaccag gatgaggagg agggaggcgt gggtgaccag aaagccgatc cgccagcggt
     3661 ttcgcggcca gcgttcccag agcgccgccc cgacgttcag ggcgaagagg cccaggagca
     3721 gccggaacca gaaggcgtgg tagacgaagc gcgccgcctc ggcgccctgg agggactcga
     3781 tgatggttcc gaacgacatc acgacggcga tggagacgag aaggatgatc gacagctcga
     3841 gcgaggccag cgtgcgcagg gtcgcgttca cggtttgggg tcccgggtgg atgggggaga
     3901 agctcgaaga cgtgcggtag cgtaccggtt tccggaagct tggaaaacgg ttccgaggta
     3961 cccctccacc cgggacgctg ggctacgcga actgcttgtc gtcggccgtg gggccgtaga
     4021 tcgagggcac gagcgcgtcg ttcaggcgca ggtagaccgt gagctgcccg cggtgatgcg
     4081 ccatgtgcat catcgtatcc cggagaacga cctcgcgcgg ctcctcgctc ttcacctcgc
     4141 cgcccgcgag caggcgccag ggcgtcttca agtgctcatc gctcgcggcg cgcagcgtcc
     4201 ggcgcgcctc ctccaccgcc ttatcgtgcg cctcgatcag ctcccggcgg gtgcgaaggg
     4261 gctttcgatc cggatggccc gctttcggcg ccacgtccag ctcgtcctga ttcacgatca
     4321 ggttgaccca ggtcggtatg gttgccacca gcatggccag acggcccagg ggcatcgatt
     4381 tgtcgtgcgg cttccagtcg tcgcgacctt cggggacatg ttccagggca cgccgggtgc
     4441 ggggtgcctc gctctccagc tgggccagaa gccattcggt cgttttcata gaatctctcc
     4501 tttcggatcg gtggggtcac tcaccgtccc ggtccgtaca gggccggagg aaaccgcggg
     4561 tgcgcggggg ctccgggcgg gtccgggtcc gtggtggtaa ccgacgtatt gagagagtgt
     4621 tcggaaccgt ccgacgtcct gggtgctagc gcccagacgc tcccgggcat ttttcttgcc
     4681 gcattcgtgc tcgtcccagt gcccagctat cgatcctacc ggaatagctc acgggcaacg
     4741 cggctcgaag acccattgtg gaggaggaga tttccatgat ccgcaagagt gtggccatcg
     4801 tcctggcggt cgtctgcctg gcactactgg gcgccgccgg ggtgacctac tccaagtaca
     4861 agaagtctca ggccggcctc gcccaggcga gtgccgatca ggaagccatg cggcagcggt
     4921 acgatcaggc cgtcaacgag atcgtctcga tccaggacag cctgaacgcc atcgccatcg
     4981 gcgagaacca tgcgcagggc gacatcgagc tccacggcca gaccattcac gagaaggtgc
     5041 tcgatcggat cacgaccatc aaggcgtcga tcgcgcgaac caaggcgcgc atcgacgagc
     5101 tggacgcgag gctgaagcac aacggcatcc gcatggcgag catgaagcgg atgatcgaag
     5161 ggctcaagga atcggtccag tatcgtgagg acaagatcgc catgctgtcg acgcaggtgg
     5221 acacgcttcg gacccgcgtg accgggctca agttcgagat ccagggacag caggaagccc
     5281 tgcagcggaa ccaggacgag ctcctggccc ggcagaagga actggcgacc atctactaca
     5341 ccatggggac caaggacgag ctgaagaatg ccggcgtggt gcagcccaac ggcggcgtgc
     5401 tcggcgtggg caagaccctc aaggtgtccg gacggttcga ggaggccgcc ttctccccgc
     5461 tcaacacgga cgagcagaac gtgatccaga tccccgcgaa gaaggggaag gtccagatcc
     5521 tgagcgcgca gccgccgggc agctactcgc tgaccaaggt ggggaaggat atggtcgagc
     5581 tgcacatcct cgaccggacc gagttccgga agatcaagca cctggtcatt ctcaaggcat
     5641 agccgcaggc agagcgacgt cgccgcgccg gcttcgtccg acgcggcaga gcgggcgggt
     5701 gtccctcacg ggcgcccgcc cgtttcgcgt cccggcgtac cggttcccgt caggcctcgg
     5761 agctgttctc gcggatcgcc gtgatcacgg cgtgccccgc gtggaagacg gccgacagcg
     5821 ggtgccggtt ctggctcagc tcctcgtcgg tcgcctcgat gccgagggtt ccctcgaagg
     5881 tcctcacgta gagccgcacg cgcccgatgc gcggcgtggg cgtgtcctcc acggaaggga
     5941 gggccggggc atgcgtctcg gccgcgtcca ggaacgcctc cgccgcctcg cgcacggccg
     6001 cgttctcccc ggccccgatc accccgccgc cgttgctgaa gtagaggctc gtcgtgccgt
     6061 cggccagtga aagcagggtg gcgatgccgg tggcatagcc cagctccatg atcgcacccc
     6121 agacgcgggg tcgcgcttcg gtcggctcga ggtccagatc ttcggccgtg gcgctcaggg
     6181 cataggcgcg cagctggcgc atcgttacgg cggggtcggt catgcgcgga tcatagcagc
     6241 gggatcccgc ggcaaatcgc gcttcctcgc ggggcgccct ccgggtagcc tcgacccccg
     6301 atgccccttt gcccctcctg ccaacacgag gtcgtgccac cggcccgctt ctgcaccgag
     6361 tgcggcgcgg cgttgaccgc tgccggcgcg acgcggtcct cgcccgactc ccatccctcg
     6421 tcgcaaagct cgtcgtcgat ccacgggcgc ttcgagcccg gcacccggct gggtacgcgg
     6481 tatcgcgtcg tcgggctttt gggacggggc gggatggggg aggtctatcg ggccgacgac
     6541 ctggagctga accagtcggt cgcgctcaag ttcctgcccg agaaggtggc gcggaaccca
     6601 tccgacctcg ccaggctccg ccaggaagtg cggatcgccc gccagatcgc gcatccgaac
     6661 gtctgccgca cgtacgacat ctccgaggcg ggcggccagg ttttcgtggt gatggagtac
     6721 gtggacggcg aggacctcgc gtcggtgctt cgccggctgg gacggccgac cccggataag
     6781 gcgctcgaga tcgcccggca gctctgcctg gggctgggcg cggcgcacga gaacggcgtc
     6841 ctccaccggg acttgaagcc cgcgaacatc atga
//
LOCUS       DASSLZ010000325         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_730116, whole genome shotgun sequence.
ACCESSION   DASSLZ010000325 DASSLZ010000000
VERSION     DASSLZ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730116"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /gene="aroC"
                     /locus_tag="VFM00_12250"
     CDS             complement(<1..574)
                     /gene="aroC"
                     /locus_tag="VFM00_12250"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET7499062.1"
                     /translation="MMGSTFGRLLTITTFGESHGPAVGVILDGMPPGIAVDPAMIQKD
                     LDRRKPGQSAVTTQRKEPDQVEILSGVFEGVTTGASIALVVRSVDARSQDYAELRDLY
                     RPGHADFTYWEKYGVRDYRGSGRSSGRETVGRVAAGAVARRLLEREGVRVQACTVQVG
                     DLVARDRDYDFVEQNPVRCPDRAVAPEMERA"
     gene            complement(571..1017)
                     /locus_tag="VFM00_12255"
     CDS             complement(571..1017)
                     /locus_tag="VFM00_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499063.1"
                     /translation="MPKATPQDAELILKLYELRRDPELRRARNYLLSEFNVTDWEEIR
                     PHWMTGNEMDRHIRMVSSYWEMVASFVNRGLLDEDLFFDTHGEALVVWNRLKAIVPAA
                     RKQIRPTWLWNLERLARRQEQWRERSFKTAARVIEAGSKLRAAKKS"
     gene            1234..1653
                     /locus_tag="VFM00_12260"
     CDS             1234..1653
                     /locus_tag="VFM00_12260"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HET7499064.1"
                     /translation="MKYIVNTDGAARGNPGPSAIGIVVRDESGRVIYEASRCLGVNTN
                     NEAEYMALIGALEYLKESRIGEAEFLLDSELVVRQLQGRYRVKEPRLQQLYARTTMLL
                     NAVPKHSFRHVPRKENARADELANEALDAQEGPPRGA"
     gene            1729..2094
                     /gene="rnpB"
                     /locus_tag="VFM00_12265"
     ncRNA           1729..2094
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFM00_12265"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2131..>2817
                     /locus_tag="VFM00_12270"
     CDS             2131..>2817
                     /locus_tag="VFM00_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7499065.1"
                     /translation="MSAKSTTPEAAGASSAAAPSAITIGPYTVHFLSGGRFRLDGGAM
                     FGVVPKVLWNRTAPADERNRIRMAMNCLLIEGDGKRVLVDAGSGTKDDAKFRDIYAIE
                     RPEGLIEDLRAAGIAPEQVDTVALTHLHFDHCGGGTSRGEDGVVRPTFPKAHYLVRRQ
                     EWDDAHHANERNRASYLSDNYDPLAQSGQLILHAQDIEILPGVWMKNLPGHTLGHQGV
                     FFDVPGARALY"
BASE COUNT          495 a          938 c          970 g          414 t
ORIGIN      
        1 tcgcgcgctc catctcgggc gccaccgcgc ggtcggggca gcgcacgggg ttctgctcca
       61 cgaagtcgta atcccgatcg cgcgcgacca ggtcgccgac ctggaccgtg cacgcctgca
      121 cgcgaacgcc ctcacgctcc agcaggcgcc gcgcgaccgc gcccgccgcc acgcgcccca
      181 ccgtctcgcg tccgctcgag cggccgctgc cgcgatagtc gcgaacgccg tacttctccc
      241 agtaggtgaa gtcggcgtgg cccggccggt agagatcgcg aagctcggcg tagtcctgcg
      301 agcgggcatc cacgctgcgc accacgagcg cgatcgaagc gcccgtcgtc acgccttcga
      361 agacgccgga gaggatctcg acctggtcgg gctccttgcg ctgggtggtg accgcgctct
      421 gtcccggctt tcggcggtcc aggtccttct ggatcatcgc gggatcgacg gcgatcccgg
      481 gcggcatgcc gtccaggatc acgccgaccg cgggtccgtg gctctcgccg aacgtggtga
      541 tcgtgaggag gcggccgaag gtgctcccca tcaggacttc ttcgccgcgc gaagcttgga
      601 tcccgcctcg atcacgcggg cggccgtctt gaacgaccgt tcccgccact gctcctgacg
      661 tcgcgccagg cgctcgaggt tccagagcca cgtgggccgg atctgcttcc gcgcggcggg
      721 cacgatcgcc ttcaggcgat tccagaccac cagcgcttcg ccgtgcgtgt cgaagaagag
      781 gtcctcgtcg agcaggccgc gattcacgaa ggaggcaacc atctcccagt aggaagagac
      841 catccggatg tggcggtcca tctcgttgcc ggtcatccag tgcgggcgga tctcctccca
      901 gtcggtgacg ttgaactcgg agaggagata gttgcgcgcg cggcggagct cgggatcgcg
      961 gcgcagctcg tagagcttga ggatcagctc ggcatcctgc ggggtggcct tgggcatttc
     1021 gcctccgggt aaacggcggg aacgcgtggc attgtcgccg tggttcaagg cgttgtcaaa
     1081 cgcgggggat cgatcagcgt cgttcggccg aactcgcggt cgcgtacttg ggcgaggcat
     1141 aggagcccga ccacccgcgc aggcgcgggt gaatttggcg acagatgtca gggctcattg
     1201 ccaaacggcg gcttcgcacg tactctgcgg cgcatgaaat acattgtgaa taccgacggc
     1261 gccgcacgag gaaatcccgg accctccgcc atcggtattg tggtgcggga cgagtcggga
     1321 cgagtgatct acgaggcgag ccgctgcctc ggcgtcaaca cgaacaacga agccgagtac
     1381 atggcgctca tcggggcgct ggagtacttg aaggaatcgc gcatcgggga ggcggaattc
     1441 ctgctcgaca gcgagctggt ggtgcgccag ctgcaggggc gctaccgggt gaaggagccg
     1501 cggctgcagc agctgtatgc acgcacgacg atgttgctga acgcggtccc caagcactcg
     1561 ttccggcacg ttccaaggaa ggagaacgcg cgcgccgacg agctcgcgaa cgaggcgctg
     1621 gacgcccagg agggaccgcc gcggggagcc tgacgtgccc gcgcgggtgc cggggaaacc
     1681 gcatccaatg tgctagactg gccccagcgt aacaggcagg cggacgtcgg agcaccacag
     1741 gcggccgcgg gccgcgctca cgcgcggttc gaggaaagtc cgagctccac agggcagggt
     1801 gctgggtaac gcccagcggg agcgatccca gggaaagtgc aacagaaagc aaaccgcctc
     1861 gggccgaaag gtccgcggta agggtgaaac ggtgcggtaa gagcgcacca gcggaggagg
     1921 cgactccttc ggctcggtaa accccacccg gagaaagacc aaatagggga cgatgaggtg
     1981 gcccgcctcg acgagtcccg ggtaggtcgc tagaggcgtt cggtaacgcg cgtcccagag
     2041 aaatggtcgc cacggagcaa tccgacagaa ctcggcttac ggggcgctcc ggcttcgccg
     2101 cctgaaaacg tccatgcgag aatcgcgaca atgagcgcca aatccacgac acccgaggcc
     2161 gcgggcgctt cctccgcggc cgcgccctcc gcgatcacga tcggccccta caccgtgcat
     2221 ttcctctcgg gcggacgctt ccgcctggac ggcggcgcca tgttcggcgt cgtgcccaag
     2281 gtcctctgga accgcaccgc gcccgcggac gagcgaaacc gcatccgcat ggccatgaac
     2341 tgcctgctga tcgaagggga cgggaagcgg gtgctggtgg acgcgggatc ggggaccaag
     2401 gacgacgcga agttccgcga catctacgcc atcgagcgtc ccgagggcct gatcgaggac
     2461 ctgcgcgccg cggggatcgc gcccgagcag gtggacaccg tcgcgctcac gcatcttcac
     2521 ttcgaccact gcggcggcgg cacgtcgcgc ggcgaggacg gcgtcgtgcg tcccaccttt
     2581 ccgaaagcgc actacctggt gcggcggcag gagtgggacg atgcgcatca cgccaacgag
     2641 cggaaccgcg cctcctacct ctccgacaac tacgacccgc tcgcccagtc ggggcagctc
     2701 atcctgcacg cgcaggacat cgagatcctg cccggcgtct ggatgaagaa cctgccgggg
     2761 cacacgctgg ggcaccaggg cgtcttcttc gacgttcccg gcgcgcgggc cctctac
//
LOCUS       DASSLZ010000326        16235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1262423, whole genome shotgun sequence.
ACCESSION   DASSLZ010000326 DASSLZ010000000
VERSION     DASSLZ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 16235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16235
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262423"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFM00_12275"
     CDS             complement(<1..356)
                     /locus_tag="VFM00_12275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7499066.1"
                     /translation="MFASVARSYDRANQVLSLGMHHRWRRAAVRSSGAAPGDRVLDCA
                     TGTGDLAIEFRRAVGPEGEVIGADFCEEMLALGPAKAARAGAPVRFEVADLLALPYAD
                     GAFDVVSVAFGIRNVE"
     gene            484..1755
                     /gene="ispG"
                     /locus_tag="VFM00_12280"
                     /gene_synonym="gcpE"
     CDS             484..1755
                     /gene="ispG"
                     /locus_tag="VFM00_12280"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET7499067.1"
                     /translation="MPLIRLSPAEPSSPADRPRRRSTEVRIGSVTIGACRPVAVQSMT
                     NTETADPASTAAQVIALAEAGSELVRVTVNTPEAAKAVPEMVRRVRDAGHATPIVGDF
                     HFSGHLLLAEHPDCAKALDKYRINPGNVGAGKRHDEHFQRIIRAAVEHEKPVRIGVNW
                     GSLDRELLTALMDENARSASPRPDRDVTLDAMFQSAVRSASLAEEFGQPHDAIVLSAK
                     VSGVRDLIAIYRRLAAETDYALHLGLTEAGMGMKGVVASTAGLSVLLLEGIGDTIRAS
                     LTPTPGGDRREEVHLCQQILQSLDLRAFAPQVTSCPGCGRTTSSFFQELAERVNDRLR
                     ERMPVWRERYPGSEELRVAVMGCVVNGPGESRHADIGISLPGTGENPKAPVYVDGSLK
                     TTLSGARIAEEFLDLVDAYVEDRYGKAATLR"
     gene            complement(1762..2553)
                     /locus_tag="VFM00_12285"
     CDS             complement(1762..2553)
                     /locus_tag="VFM00_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499068.1"
                     /translation="MLFRVTLAVLALLIAARAGHEQRSQLPAWPLAALFAATAAALAI
                     WPLLVPSRPRILAPHSLAPAFYIAGMFLLPPAALVAMIAFAITLSDLIRGIRAYRIVF
                     DLSTAILAYVAPALLFSLGPRPAEIMFQPAARAGLELMIAASAVTLHLLLRSVALRLE
                     HGEETPRWGAFEGATLIEAIYGLVISVTILVLARIHPALLAVVYVQLGITTWLVRRYR
                     AYVVELREEAERPKRRLKAIGERSSDGARREDAGRWWGKRASGSR"
     gene            2846..3259
                     /locus_tag="VFM00_12290"
     CDS             2846..3259
                     /locus_tag="VFM00_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499069.1"
                     /translation="MQEESTTEHSLERRIERLERQNRRYRLGGAILVLASAAALCIGM
                     GAPRKTLETDLLLIKDANGTTRMILGMADDGPAITMLDEKGKLRANIGVTAKGPEFDF
                     LDASETPRLSMSIDDKQVPRLNLLDNKGTQVVFRP"
     gene            3364..4260
                     /locus_tag="VFM00_12295"
     CDS             3364..4260
                     /locus_tag="VFM00_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7499070.1"
                     /translation="MRPETGALPAEERGPATYASFAVMCLIWGSTFLAIRIGNESVAP
                     VWSAVVRLAIATPLYFLIALLSRAPWPRGAALGAALLYGFLNYGVNFALLYRGEVEVS
                     SGTASILYAMIPLITVIAVARLGLQRLRAHEVLGALIGLAGVALVFSGELSGGGPPLA
                     LAAVFVGAIAAALSAVALKLGPPQSTWPANALGSAVGLGVCCAISLALGERWTLPRGT
                     AGWGPILYLIATGNLIAYALFGWLLTKWRVTSVNAMSLVIPVIAVLLGAAIRAETPGP
                     GTVLGAALVLAGVSLTLFVGRR"
     gene            4296..4661
                     /locus_tag="VFM00_12300"
     CDS             4296..4661
                     /locus_tag="VFM00_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499071.1"
                     /translation="MKMSNVRFRTRHGAVLAALLVALALSLAGCKGATKIKTLLDDPG
                     RFDGTVVRVAGKVTTSVGLLGYGAYKLNDGTGTILVVSKHGVPREGAEVGVEGTFHSV
                     FTFHDEAGAAIEEAARKER"
     gene            complement(4682..5629)
                     /locus_tag="VFM00_12305"
     CDS             complement(4682..5629)
                     /locus_tag="VFM00_12305"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7499072.1"
                     /translation="MNERNDSRSGPVLLTGGSGFVGSRVARRLVAAGRRVRAIVRAKG
                     AAPELADPAVSGSIEEIEGDFTRPEVAGPAAAGASAVVHCAATGGPDMETARRVNVEG
                     TRAMVEAAERAGVRQYVQISTMSVYVRGPEGTLDEDAPLKQEGDPYGFTKAEADRVVL
                     AAMERGLPAVILRPGAILGAHRTSTWAVAMPARIRDGQVKLRGDGREIIPWVHVDDLA
                     EAVLLALDDDRAVGRVYNMTDGGMTWRRYTDDVRSWFGTAPLVETPVAEFGGYWMGDF
                     DASRIRRELGYRPRHGYDEGMAEAAAHWARERASRQPSR"
     gene            5659..6078
                     /locus_tag="VFM00_12310"
     CDS             5659..6078
                     /locus_tag="VFM00_12310"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HET7499073.1"
                     /translation="MRRTKDHLTSIGLLLLRVGGGALLLFGHGLPKLLHWSERVSGFA
                     NPIGLGSQVSFSLVVFAEVVCAAAVVLGVATRLAAGPIIFFLLVAAFVQNGPGSFSDK
                     ELALVYLVPFVTLTLTGGGHYALDTLILRGRAGRRRL"
     gene            complement(6075..6842)
                     /locus_tag="VFM00_12315"
     CDS             complement(6075..6842)
                     /locus_tag="VFM00_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578449.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET7499074.1"
                     /translation="MTHEDDAAPPRGGWLPWAALALGIALAGWFVGMGVQNVRTADRF
                     VSVKGVAEREVKADLALWPIQLAVTDNDLSAAQTRLAQNAARVVAFLRENGIDSTEIE
                     LQTLRVTDIMANPFRGEQRQGTRFILQQVVNVRSDNPERVQATSQKVGELVNAGVVLT
                     SGPEWGPGGPSYLFRKLNDLKPGMIAEATAQARKAAEQFAKDSRSRLGGIHRANQGVF
                     VILPRDASGSDQGPAIAEATQMYKIVRVVSTVEYQLR"
     gene            complement(6994..7257)
                     /locus_tag="VFM00_12320"
     CDS             complement(6994..7257)
                     /locus_tag="VFM00_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499075.1"
                     /translation="MKRFGLTLYRRTMFGLTAAATLLFVPPAFGQDSTSVSTSHTSVT
                     TSTSSEFVSDWRLWALVGAVVLIILIIALTRRGSGGDTTTIVK"
     gene            complement(7323..7601)
                     /locus_tag="VFM00_12325"
     CDS             complement(7323..7601)
                     /locus_tag="VFM00_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499076.1"
                     /translation="MKRCALAVHRLGHSLWMALLLALVGVPAWAQQGGGGGGNGDVSV
                     STTTTETTSAASWTSDWRIWALVGVVVLVILVIALTRGRGGSNTTVVK"
     gene            complement(7693..9303)
                     /locus_tag="VFM00_12330"
     CDS             complement(7693..9303)
                     /locus_tag="VFM00_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7499077.1"
                     /translation="MKSFRTIVFALSLAVLGGAWFSRPAQSEPASLHLNITPFGGYSD
                     FAKEVNLDDRPEFGGRLGIGFGRYIGIEGYYAWMSTHTKSGSADSLFVAGPAGRPEEN
                     VTVSRYGADLILNLIPSSGFNPYILGGWFEDQVKTKDRATDKKQFFNGIEFGGGVKLG
                     LSKRAALRLEVRDKLWSFGDGAGIPNPPGNDNLSNLVYTGGIQLTLGGLTSHDEDKDG
                     IKDKDDKCPGTPLGARVDESGCPIDSDHDNVPDGIDQCPNTPQGATVDARGCPTDSDN
                     DGVPDGIDQCPGTPANTSVDARGCPADADGDGVPDSQDQCANTPQGAQVDERGCPKDS
                     DNDGVPDGIDQCPNTPANTQVDARGCSTDSDNDGVTDDKDLCPNTQANVKVDKDGCPI
                     ELTEKETELLDKGRITTREIHFETAKWDILPESRPVLDEIGKVLIQWPQLKIEIGGHA
                     DARGSDAYNMDLTQKRAQAVLDYLVQNFPQIKGDQYTAVGYGERKPVASNKTVEGMAK
                     NRRVEFKVLNTEELKKERERRRIMQKGE"
     gene            9629..9853
                     /locus_tag="VFM00_12335"
     CDS             9629..9853
                     /locus_tag="VFM00_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499078.1"
                     /translation="MKGLVIVGVLLIVLGLAGLLAGGLTYTKDKEKADLGPVDITVTE
                     KKHVPVPPAVGAIAVVAGVVLVVAGMRRTT"
     gene            complement(9846..14138)
                     /locus_tag="VFM00_12340"
     CDS             complement(9846..14138)
                     /locus_tag="VFM00_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HET7499079.1"
                     /translation="MPHPFRRHVLSARAAIFAAASFFLVVTATPSRAADVATDNPDYY
                     PGDVVVITGSGFLPGERVSLRLDQHPTLHDPYVFYSTADSIGSFRNADYTVEDQDLGT
                     SFDLVATGLTSGIVATAFFTDSPKVGSVSIGMQNGSLCAGSHGSATFTVTVNRGSGGG
                     SSGSFTALLSIFGDLHPGITASFFPAIVRFQSGQASATSTLTLSTDGSAAVGPMGFAV
                     RAATSTNDFAIGDGGLYEVGAPAVLQCPADVVVNNAPGQCGAVVNFPPATATGSPIPA
                     ITYSADPGSFFPVGVTHVCVSAVNSCASAACEFTVTVVDVEPPSIQVADITQSTDPGT
                     CVATVSALGASAADNCGVVSLIGTRSDGAFLTAPFAKGTTTVTWVATDVSGKTATVLQ
                     HVVVNDTQPPTIQAPPALNLTTGPAATACGVRVADAGLISASASDNCGASISSTGIPE
                     NNFFPVGTTTITYTATDPSGNTAVGTQTVTVVDDTPPTIAVSNLDLSADAGGCAATIG
                     SLGASANDNCGVDTFVGTRGDGAALTDPFPKGATTITWLATDVHGNTQTATQTVTVAD
                     DEAPTVTASDLSLSTDPGSCGATIASLGAAATDNCGVASFIGTRSDGLPLTDPFPRGT
                     TTIAWVATDTSGNKAGAKQHVMVADTEHPTLTAPASLNVTTGPGSQTCGVGVGNAALG
                     TPVASDNCAVTVTVEGIPAGNLFPVGVTTLTWTATDPSGNATMATQTVTVVDDTPPFV
                     TGGESTHATDANSCGAVIGAFGVVQSDNCGVQSVVGTRDDGLALSDPFPVGLTTIHWL
                     ATDIHGNTATGTSRIHVEDHEAPSIAAPAAVQASTGSGARICGAVISDAALGQPVATD
                     NCSVVVTRAGVPAGNLFPTGVTVITWTATDPSGNATSATQAVTVVDNTPPTLSVPPSV
                     SFGTGPNATICGLTVTDAMLGAATASDNCSVTLTRTGVPAGNLFPTGVTAVTYTAADP
                     SGNTVTLTQLVTVTDTTPPKVAASNITLPTDAGLCSAAIANLGATATDNCGSPTLSSA
                     RSDTKSLTDPFPKGVTTILWTATDAAGNVSTATQTVTVTNATPVAEITAPSSGLVVPV
                     NTPVSLTGTFTDDAGDLHTASWTCDALACPASVNEDSRTASGSMTFATAGVYRIKLVV
                     TDQCGGSSTAVTVGGLDAMVVVYDPSAGFVTGGGWIQSPAGSYVPDPSLAGKANFGFV
                     SKYKKGTTVPTGETEFQFKAGNVDFHSSSYDWLVISGARAQFKGTGTINGAGTFGFLL
                     SAVDGETAGGGGRDKFRIKIVDKVTSQVVYDNMMGAPDTSAAATLLGGGSIAIQTNGG
                     KASASIGASLDAGAASPAASGLAQNRPNPFNPETTLRFTLARSARATLRVFDARGALV
                     RTLVDDMLEAGAHEARWNGLDARGGRVPSGVYYALFSASDGTRDRVRMVLIK"
     gene            complement(14257..14973)
                     /locus_tag="VFM00_12345"
     CDS             complement(14257..14973)
                     /locus_tag="VFM00_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HET7499080.1"
                     /translation="MKPHWKTELPLLALIAGMFVAAALIWPHAPESFPVHWGVSGEVD
                     RYGGRFEGLLFLPLLSVALYLIMLFLPRLDPGRLNYARFAGAYYTIRATTIALMALLY
                     TVMVLAAKGTPVDMPRFAGLAIGAMFFVMGNVLGKIRPNWFVGVRTPWTLSSKRSWTR
                     THRLAGWVFVVGGIALMAAGIVHTPVAMEAAFAILATGMVGAIVYSYFVWKSDPDRVP
                     PAGTLPGNDEPGAEGIRPGR"
     gene            complement(14970..15254)
                     /locus_tag="VFM00_12350"
     CDS             complement(14970..15254)
                     /locus_tag="VFM00_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HET7499081.1"
                     /translation="MGIDEVFRALSDPTRREILRLLRSGDKTAGEIAERFELAKSTMS
                     GHFNVLKHAGLVVSERSGTTIVYSLNLAAYEEALAGVMSLLGVGRKEKRA"
     gene            complement(15463..15741)
                     /locus_tag="VFM00_12355"
     CDS             complement(15463..15741)
                     /locus_tag="VFM00_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499082.1"
                     /translation="MSGGAVAAAAAARMRAIQASGAIVRLDASEFERLLIHNPGGIVV
                     HAPGGFLMGGHRYLTSYKGLVFSTKSREPLSLPGASEIVEAGRIWTPH"
     gene            15765..>16235
                     /locus_tag="VFM00_12360"
     CDS             15765..>16235
                     /locus_tag="VFM00_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499083.1"
                     /translation="MTGCLSAGCSSREAQHDTGTLAAEHSIPLTTVEFAGHRYVARVD
                     VGLGETVPLMIHGNSRMFLSLTHAIGAKVNGAPVRATERYGYSSKGRGSLRVARVRLG
                     GATFPEIPPVPVFDFTENGDTTVQGMLGVPFLVAARAAVDFSRDRLLLGVAERAE"
BASE COUNT         2427 a         5567 c         5597 g         2644 t
ORIGIN      
        1 tcctcgacgt tccggattcc gaacgctacc gagaccacgt cgaaggctcc gtccgcgtac
       61 gggagcgcga ggaggtcggc gacttcgaag cgcacgggcg ctccggcgcg cgccgccttg
      121 gcgggcccca gggcgagcat ctcctcgcag aagtcggcgc cgatcacctc gccctcgggg
      181 cccacggcac ggcggaactc gatcgcgaga tctccggtgc cggtcgcgca gtcgagcacg
      241 cggtcgccgg gcgcggcacc gctcgagcgc acggccgcgc ggcgccaacg atggtgcatc
      301 cccagcgaga gcacctggtt cgcgcggtcg tacgagcgcg cgaccgaggc gaacatggat
      361 cggacttcgg aggcggcggg catgcggcgg agtgtaccat cagccagcgc gctcccggac
      421 gcgctggcgc ccggtccgcc cgcctccttt gacccgccgc gccgcgggcg ctagactgag
      481 tcgatgcccc tcatccgatt gagccccgcg gaaccctcga gtcccgccga ccgcccccgc
      541 cgccgttcca ccgaggtccg gatcggctcg gtcacgatcg gcgcctgcag gcccgtcgcg
      601 gtccagtcga tgaccaacac cgagaccgcc gaccccgcgt ccaccgccgc gcaggtgatc
      661 gcgctggccg aggcgggctc ggagctggtg cgggtcaccg tgaacacccc cgaggccgcg
      721 aaggcggtgc cggagatggt gcgccgcgtc cgcgacgccg ggcacgcgac ccccatcgtc
      781 ggcgatttcc atttcagcgg gcaccttctc ctcgcggagc atcccgactg tgctaaagcg
      841 ctcgacaagt accgcatcaa tccggggaac gtcggcgcgg gcaagcggca cgacgagcac
      901 ttccagcgga tcatccgggc cgcggtcgag cacgagaagc cggtgcggat cggcgtgaac
      961 tggggctcgc tcgaccgcga gctcctgacc gcgctgatgg acgagaacgc ccggagcgct
     1021 tccccgcgtc ccgaccgcga cgtgactctg gacgcgatgt tccagagcgc cgtccgctcg
     1081 gcctcgctgg ccgaggaatt cgggcagccg cacgacgcca tcgtcctttc ggccaaggtc
     1141 tccggcgtgc gcgatctgat cgcgatctac cggcgcctgg ccgcggaaac cgactatgcg
     1201 ctccacctcg gcctgaccga ggccggcatg gggatgaagg gggtcgtcgc ctcgaccgcg
     1261 ggactctcgg tcctgctgct cgaagggatc ggcgacacga tccgcgcgag cctcaccccc
     1321 acgcccggcg gcgaccggcg cgaagaggtg catctctgcc agcagatcct ccagtcgctc
     1381 gacctccgcg ccttcgcgcc gcaggtcacc tcctgccccg ggtgcgggcg caccacgagc
     1441 agcttcttcc aggagctggc ggagcgggtg aacgaccggc tgcgcgagcg gatgccggtc
     1501 tggcgggagc gctacccggg atcggaggag cttcgcgtgg cggtgatggg ctgcgtggtg
     1561 aacgggcccg gggaatcccg gcacgccgac atcggcatct cgcttccggg gaccggcgag
     1621 aatccgaagg cgcccgtcta cgtcgacgga tcgctcaaga ccaccctctc gggagcccgc
     1681 atcgccgagg agtttctgga cctcgtggat gcgtacgtcg aagaccgcta cggcaaggcg
     1741 gccacgctcc gctgacgccg gctagcgcga gccgctcgcg cgcttgcccc accagcgtcc
     1801 ggcatcttcc cttcgcgcgc cgtcgctcga gcgctccccg atcgctttca ggcgccgctt
     1861 cggccgctcc gcctcctcgc gcagctccac gacgtacgcg cgatagcgcc gcaccagcca
     1921 ggtcgtgatc cccagctgga cgtacacgac ggcgaggagc gccggatgga tccgggccag
     1981 gaccaggatc gtcaccgaga tcacgagccc gtagatcgcc tcgatcaggg tcgccccctc
     2041 gaacgcgccc cagcgcggcg tctcctcgcc gtgctccaaa cgcagggcca cgctgcgcag
     2101 caacagatgg agcgtcaccg ccgaagccgc gatcatcagc tccaaccccg cccgcgccgc
     2161 cggctggaac atgatctcgg ccggccgggg acccagcgag aagaggagcg ccggggcgac
     2221 gtaggccagg atggcggtgg agaggtcgaa gacgatccgg taggcgcgga tgccgcggat
     2281 caggtcggag agggtgatcg cgaaggcgat catcgcgacg agcgcggcgg gcgggagcag
     2341 gaacatcccc gcgatgtaga acgccggagc cagcgagtgc ggcgccagga tgcgcggccg
     2401 cgacgggacg aggagcggcc agatcgcgag cgccgcggcg gtcgccgcga agagcgcggc
     2461 gagcggccat gcgggcagct ggctccgctg ttcgtgcccg gcgcgcgcgg cgatgaggag
     2521 cgcgagcacc gccagcgtca cgcggaacag cacgatctgg atgtagcgtc gggaaatcag
     2581 cggaggcgcc tccctggaat ggaccccttc ctatgccagg agtatcggcg cgatcggcct
     2641 gcgacttgag gccgcaacga attgccgctc cataggttgc caagggaccg atcgcgccga
     2701 ccaacgggcc ggcggaccga aaccgcctaa acttttcgcc tctcctgccg attcattgct
     2761 gtcgccgtgc cgcgcgcgac gttcctcgcg tggcccgatg cgaccagggt tcgaaacttc
     2821 ccactcgaat cgaaaggggc ggtgcatgca ggaggaatcg acgacggagc attctttgga
     2881 gcggcggatc gaacggctgg agcggcagaa ccgccgctat cggctcggcg gcgcgatcct
     2941 ggtcctggcc agcgcggcgg cgctgtgcat cggcatgggc gctccccgga agacgctgga
     3001 aaccgatctg ctgctcatca aggacgcgaa tggaaccacc cggatgatcc tgggcatggc
     3061 cgacgacggg ccggcgatca cgatgctgga cgaaaaaggg aagcttcgcg cgaacatcgg
     3121 cgtcaccgcg aagggtcccg agttcgattt tctcgacgcg tcggagacgc cgcggctctc
     3181 gatgtcgatc gacgacaagc aggtcccccg cctgaatcta ttggacaaca agggtaccca
     3241 ggtcgtgttc cgcccgtagg aagcgccccc gcttgcgacg ggccggtccc acccggggcc
     3301 ggcccgattt cgtttccgcg cgcgtatttc ctccggcgct cccctctgtc aagatgcgcc
     3361 accatgagac ccgagaccgg cgctctgccc gccgaggagc gcgggccggc cacctacgcg
     3421 agcttcgccg tgatgtgcct gatctgggga agcaccttcc tcgcgatccg gatcggcaac
     3481 gagtcggtgg cgccggtctg gtcggccgtg gtgcgtctgg ccatcgcgac gccgctctac
     3541 ttcctgatcg cgcttctctc gcgcgctccc tggccccgcg gcgccgcgct gggcgccgcc
     3601 ctcctctacg gattcctgaa ctatggcgtg aacttcgcgc tcctctaccg cggcgaggtc
     3661 gaggtctcga gcggcacggc gtcgatcctc tacgccatga tcccgctgat caccgtgatc
     3721 gcggtcgcgc ggctcggcct ccagcggcta cgggctcacg aggtcctggg ggccttgatc
     3781 gggctggccg gcgtcgctct cgttttctcg ggagagctct cgggaggcgg tcctccgctc
     3841 gcgctggcgg cggtcttcgt gggtgccatc gcggcggcgc tttcggccgt ggcgctgaag
     3901 ctgggcccgc cccaatccac atggcccgcg aacgcgctcg gctcggcggt gggcctcggg
     3961 gtatgctgcg ccatcagcct ggcgcttgga gagcggtgga ccctgccgcg cggcaccgcg
     4021 ggatggggcc cgatcctcta tctgatcgcg acgggaaacc tgatcgccta tgcgctcttc
     4081 ggctggcttc tgacgaagtg gagagtcacg agcgtcaacg cgatgtcgct ggtcatcccg
     4141 gtgatcgcgg tgctcctcgg cgcggcgatc cgcgccgaga cgcccggccc cggaaccgtg
     4201 ctcggcgccg cgctcgtgct cgcgggtgtc tcgctcacgc tgttcgtcgg ccggcggtga
     4261 tcgccgccgc gccgacgacg acccctggag gtgtcatgaa gatgtcgaac gttcggttcc
     4321 gcacgcgcca cggcgcggtg ctcgcagcgc ttctcgtcgc cctggccctg agcctcgccg
     4381 gatgcaaggg ggccaccaag atcaagacgc tgctcgacga tcccggccgc ttcgacggca
     4441 cggtggtccg cgtggcgggc aaggtcacga cctcggtggg cctcttgggg tatggcgcct
     4501 acaagctgaa cgacggcacc ggaacgatcc tcgtcgtgtc gaagcacggc gtgccccgcg
     4561 aaggcgccga ggtcggcgtc gaggggacgt tccactcggt cttcaccttc cacgatgagg
     4621 cgggagccgc gatcgaggag gcggcgcgca aggagcgctg agaggcacgc acggcgccgg
     4681 ctcagcggga gggctggcgc gaggcgcgct cgcgcgccca gtgcgcggcc gcctcggcca
     4741 ttccttcgtc gtagccgtga cgcggccggt agcccagctc gcggcgaatg cgggaggcgt
     4801 cgaagtcgcc catccagtag ccgccgaatt ccgcgacggg cgtctctacg agaggagcgg
     4861 tgccgaacca gctgcggaca tcgtcggtat agcggcgcca cgtcatcccg ccgtcggtca
     4921 tgttgtaaac gcgcccgacc gcgcgatcgt cgtccagcgc gaggagcacc gcctcggcca
     4981 ggtcatcgac gtgcacccac gggatgatct cccgcccgtc gccgcgaagc ttcacctgtc
     5041 cgtcgcggat gcgggcgggc atcgcgaccg cccaggtcga ggtccgatgc gcgcccagga
     5101 tggccccggg gcgcaggatc acggccggca gcccccgctc catcgccgcc agcacgacgc
     5161 gatcggcctc ggccttggtg aagccgtaag gatctccctc ctgcttcagc ggcgcgtcct
     5221 cgtccagggt gccctccggg ccgcgcacat agaccgacat ggtcgagatc tgtacgtact
     5281 gccggacgcc cgcgcgctcg gccgcctcga ccatggcgcg agtgccctcg acgttcaccc
     5341 gccgcgccgt ctccatatcg ggcccgcccg tggcggcgca gtggaccacg gcgctcgctc
     5401 cggcggccgc cggtccggcg acctcggggc gcgtaaaatc tccctcgatc tcctcgatcg
     5461 aaccgctcac ggcggggtcg gccagctcgg gggccgcccc cttcgcgcgc acgatcgccc
     5521 gcacgcgccg tcccgccgcg accaggcgcc gcgccacgcg gcttcccacg aacccgctac
     5581 ctcccgtcag taggacgggc ccgcttcggc tatcattccg ttcgttcatc ggcggcttcc
     5641 cttctcgaaa ggagcggcat gcgtcgaacc aaggatcacc tgacctcgat cggcctcctg
     5701 ctgcttcgcg tgggcggggg cgccctcctt ctcttcggtc acggactccc caagctgctc
     5761 cactggtcgg agcgcgtctc cggcttcgcc aatccgatcg gtctcggctc ccaggtcagc
     5821 ttctcgctgg tcgtgttcgc cgaagtggtc tgcgccgcgg cggtcgtcct gggcgtcgcc
     5881 acgcgcctgg cggcggggcc gatcatcttc ttcctcctcg tggccgcgtt cgtgcagaac
     5941 ggcccgggga gcttctccga caaggagctc gctctggtct acctggtgcc cttcgtgacg
     6001 ctcacgctga ccggaggcgg gcactacgcc ctggataccc tcatcctgcg cggccgcgcg
     6061 ggccggaggc gcctctagcg cagctggtac tccacggtcg agaccacgcg gacgatcttg
     6121 tacatctgcg tcgcctcggc gatggccggc ccctggtcgg agccggaggc gtcccgcggc
     6181 aggatcacga agacgccctg gttggcccgg tggatgccgc ccaggcggct cctcgagtcc
     6241 ttggcgaact gctccgccgc tttccgcgcc tgggccgtgg cttcggcgat catgcccggc
     6301 ttcaggtcgt tgagcttccg gaagagatag ctcggcccgc ccggccccca ctccgggccc
     6361 gaggtgagca cgacgcccgc gttcaccagc tcccccacct tctgactggt cgcctgcacg
     6421 cgctcgggat tgtcggagcg gacgttcacg acctgctgga ggatgaagcg ggtcccctgg
     6481 cgctgctcgc cgcggaaggg attggccatg atgtccgtga cgcggagcgt ctgcagctcg
     6541 atctcggtcg agtcgatgcc gttctcgcgc aggaacgcga ccacgcgcgc cgcgttctgg
     6601 gccagccgcg tctgcgccgc cgacaggtcg ttgtcggtca ccgcgagctg gatcggccag
     6661 agggccaggt cggctttcac ctcccgctcg gccactcctt tgacgctgac gaaccggtcg
     6721 gccgtgcgaa cgttctgaac ccccatcccg acgaaccacc ccgccagcgc gatgcccagc
     6781 gcaagcgccg cccagggcag ccatcccccg cgcggcggcg ccgcgtcgtc ctcgtgcgtc
     6841 atcgtcccct cccctatgga actgcgcttc cggcgccggg gagtataacg aaaccggcgg
     6901 gcccccacgt gagcgggccc gccgatcttc catgtggatc cggcatctat gtccgggagc
     6961 gatgcgggtc ccttcgaacc cgcacacgcg atcctatttc acgatcgtcg tggtatcgcc
     7021 gccgcttcct ctccgcgtca gggcaatgat caggatgatg agcacgaccg ccccgaccag
     7081 cgcccagagc cgccaatccg acacgaactc cgacgacgtg ctggtcgtca cggaggtgtg
     7141 actggtgctg accgaagtag aatcctgtcc gaacgccggc ggcacgaaca gcagcgtcgc
     7201 cgccgccgtc aggccgaaca tcgtccggcg gtatagggtg aggccgaagc gtttcatgtc
     7261 gcctcctcag tcggacggcg cggagtcttg cggaactcca ccctccgtcc tcgatcgggg
     7321 gtttacttga ccacggtggt gttgcttccg cccctgcctc gagtcagcgc gatcaccagg
     7381 atgaccagga ccacgacgcc gaccagggcc cagattctcc aatcggaggt ccaggacgcg
     7441 gccgaggtcg tctcggtcgt ggtcgtggag accgatacgt cgccattgcc accgccaccg
     7501 ccgccctgct gcgcccaggc cggaaccccg accagagcga gcagcaatgc catccataag
     7561 gagtgaccga gacgatggac cgcgagagcg cagcgcttca tgagagtgct cctttctatg
     7621 gaatcacagg aggggagcct ttcggccccc ctcccgcctg ccgtgttcgg gatgatgcaa
     7681 agacaatgcc tgttattcgc ccttctgcat gatccgccgg cgttccctct ccttcttgag
     7741 ctcttcggtg ttgagcacct tgaactcgac gcggcggttc ttcgccatcc cttccacggt
     7801 cttgttggag gccaccggct tgcgctcgcc gtagcccacc gccgtgtact gatctccctt
     7861 gatctgcggg aagttctgga ccaggtagtc cagcaccgcc tgcgcgcgct tctgcgtgag
     7921 gtccatgttg taggcgtcgg acccgcgcgc gtcggcatgg ccgccgatct cgatcttgag
     7981 ctgcggccac tggatgagga ccttcccgat ctcgtccagc accggacgcg actcggggag
     8041 gatgtcccac ttggccgtct cgaagtggat ctcgcgcgtg gtgatacggc ccttgtcgag
     8101 cagctcggtc tccttctcgg tcagctcgat cgggcagccg tccttgtcga ccttgacgtt
     8161 cgcctgggtg ttcggacaga gatccttgtc gtccgtcacg ccgtcgttgt ccgagtcggt
     8221 cgagcagccg cgagcgtcca cctgggtgtt cgccggggtg ttcgggcact ggtcgatgcc
     8281 gtcgggaaca ccgtcgttat cggagtcctt gggacagccg cgctcgtcca cctgcgcgcc
     8341 ctgcggcgtg ttcgcgcact gatcctggct gtcgggaacg ccgtcgccgt ccgcgtccgc
     8401 cgggcagccc cgcgcgtcca ccgaggtgtt cgccggcgtg ccggggcact ggtcgatgcc
     8461 gtcgggaacg ccgtcgttat ccgagtcggt cgggcagccg cgcgcgtcca ccgtcgcgcc
     8521 ctgaggcgtg ttcgggcact ggtcgatgcc gtcgggcacg ttgtcgtggt cggagtcgat
     8581 cggacatccg ctctcgtcca cgcgcgcgcc gagcggggtg cccgggcact tgtcgtcctt
     8641 gtccttgatg ccgtccttgt cctcgtcgtg ggacgtaagg ccgcccagcg tgagctggat
     8701 gccgcccgtg tagaccaggt tgctcagatt gtcgttgccg ggaggattgg gaatgcccgc
     8761 gccgtcaccg aacgaccaca gcttgtcccg gacctccagg cgcagcgcgg cgcgcttgct
     8821 caatccgagc ttcacgccgc cgccgaattc gatcccgttg aagaactgct tcttgtcggt
     8881 cgcgcggtcc ttggtcttca cctgatcctc gaaccagccg ccgaggatgt acgggttgaa
     8941 tccggagctg gggatgaggt tcaggatgag gtcggcaccg tagcgggaca cggtgacgtt
     9001 ctcctcgggc cggcctgccg gcccggcgac gaacagcgag tccgcgcttc ccgacttggt
     9061 gtgggtgctc atccaggcgt agtagccctc gatgccgatg tagcgtccga atccgattcc
     9121 caggcggccg ccgaattcgg gtcggtcgtc gaggttcacc tccttggcga aatcggagta
     9181 cccgccgaag ggtgtgatgt tgaggtgcag gctggccggt tcgctttgcg cggggcgtga
     9241 gaaccacgct cctccgagca cagcgaggct cagggcgaaa acaatcgtcc ggaatgactt
     9301 catgattttc gtcctcttat tgcgggtggc tggcgactgc tcggacgctg cgtggtccgg
     9361 gcgcgactcg gggtgaacgc gccatactta ccagatgccg ccagtcccct cgtgcagata
     9421 ctgtgccaga aatgggttag cctccatgct gcaccccgat ctcgtgatgc gccaccagtc
     9481 cggaggctcc tgcttcaggc tgcacaggcg gatgcagcct agtgccgccc ggttccggtt
     9541 tcaacaatcc gtgtaaacta ttccagggcg gcccatgcca gcgcgccgcc gttcggtaca
     9601 tcgatttccg gatcggtacg gaggggtcat gaaaggactg gtgatcgtcg gcgtgctgct
     9661 gatcgtgctt ggacttgcgg ggctactcgc cggcgggttg acctacacga aggacaagga
     9721 gaaggcggac ctcgggccgg tggacatcac ggtgacggag aagaagcacg ttccggtgcc
     9781 gccggccgtg ggagccatcg cggtcgtggc gggcgtcgta ctggtcgtcg cgggaatgag
     9841 gcggactact tgatcagcac catccggacg cggtcgcgcg ttccgtcgga ggcggagaag
     9901 agagcgtagt agacgcccga cggaacgcgg ccgccgcgcg cgtcgagccc gttccagcgc
     9961 gcttcgtgcg cgcccgcctc cagcatgtcg tccacgaggg tccgcaccag ggctccacgc
    10021 gcgtcgaaga cgcgaagcgt ggcgcgggcg gaacgcgcga gcgtgaatcg cagcgtggtc
    10081 tccggattga aggggttcgg ccggttctgc gccaggccgc tcgccgcggg tgaggccgct
    10141 ccggcgtcca gcgacgcgcc gatgctcgcc gacgccttgc cgccgttggt ctggatcgcg
    10201 atcgagccgc cgcccaggag cgtggccgcc gccgaggtgt cgggcgctcc catcatgttg
    10261 tcgtagacga cctggctcgt gaccttgtcg acgatcttga tccggaactt gtccctgccg
    10321 ccgccgcccg ccgtctcgcc gtccaccgcg ctcagcagga agccgaaggt tcccgcgccg
    10381 ttgatcgtgc cggtcccctt gaactgggcg cgcgcgccgg agatgacgag ccagtcgtag
    10441 ctggagctgt ggaagtcgac gttccccgcc ttgaactgga actcggtctc gcccgtcggc
    10501 acggtcgtcc ccttcttgta cttggagacg aagccgaagt tggccttgcc cgcgagcgac
    10561 gggtcgggca cgtacgatcc cgcgggcgac tggatccaac cgccgcccgt gacgaagccc
    10621 gcgctggggt cgtagaccac gaccatggcg tccaggccac ccaccgtcac ggccgtggag
    10681 gagccgccgc actggtcggt cacgacgagc ttgatccggt agacgccggc cgtggcgaag
    10741 gtcatcgagc ccgacgccgt acgcgagtcc tcgttgaccg aggccgggca ggcgagagcg
    10801 tcgcaggtcc aggacgcggt gtgcaggtcg ccggcgtcgt cggtgaaggt tcccgtcagc
    10861 gagaccggcg tgttcaccgg gacgacgagc ccgctcgagg gggccgtgat ctcggcgacc
    10921 ggcgtcgcgt tcgtcacggt gacggtctga gtcgcggtcg agacgttccc ggcggcatcg
    10981 gtcgccgtcc agaggatcgt cgtgaccccc ttcgggaagg ggtccgtgag ggacttggtg
    11041 tcgctgcggg cgctcgagag cgtgggcgag ccgcagttgt cggtggcggt ggcgcccagg
    11101 ttggcgatcg cggcgctgca gaggccggca tcggtcggga gcgtgatgtt ggacgcggcg
    11161 accttcggcg gcgtcgtgtc ggtcaccgtg acgagctgcg tcagggtcac ggtgttcccg
    11221 ctgggatcgg ccgcggtgta agtgaccgcg gtgacgcccg tcgggaagag gttccccgcg
    11281 ggtacgccgg tccgggtcag cgtgaccgag cagttatcgc tcgcagtcgc cgcgccgagc
    11341 atggcgtcgg tcaccgtgag gccgcagatc gtggcgttgg ggcccgtgcc gaacgagacg
    11401 gacggcggca cgctcagggt gggcggcgtg ttgtcgacca ccgtgacggc ctgcgtcgcg
    11461 ctcgtggcgt tgccgctcgg atccgtcgcg gtccaggtaa tcacggtgac gcccgtcggg
    11521 aagagattcc ccgccggcac gcccgcgcgg gtcacgacga ccgaacagtt gtcggtcgcg
    11581 acgggctgac cgagcgccgc gtcggagatg acggcgccgc agatacgggc tccggatccc
    11641 gtggaggctt gcaccgccgc gggtgccgcg atcgacggcg cctcatgatc ttcgacatga
    11701 atgcgggacg ttcctgtggc cgtattgcca tggatgtccg tggccagcca gtggatcgtc
    11761 gtcagaccga ccgggaaggg atcgctcaaa gcgagcccgt cgtcccgggt tcccacgacc
    11821 gactgaacgc cgcagttatc gctctggacc acgccaaagg ccccgataac ggcgccgcac
    11881 gagttggcgt cggtcgcatg cgtgctctcg ccacccgtca cgaagggcgg cgtgtcgtcc
    11941 acgacggtca ccgtctgcgt ggccatcgtc gcattgccgc tcggatccgt cgccgtccag
    12001 gtgagcgtcg tgacgccgac cgggaacagg ttccccgcgg ggatcccctc caccgtcacg
    12061 gtcacggcgc agttgtccga ggcgaccggc gttcccaggg cggcgttccc cacacccacg
    12121 ccgcacgtct gggaaccggg cccggtcgtc acgttcagag aggccggcgc cgtaagggtc
    12181 gggtgctcgg tgtccgcgac catcacgtgc tgctttgcgc cggccttgtt gccgctggtg
    12241 tcggtggcca cccaggcgat cgtcgtcgtg ccccgcggga acggatcggt cagggggagc
    12301 ccgtcgctgc gcgtgccgat gaacgaagcg acgccgcagt tgtccgtcgc ggccgccccg
    12361 agcgacgcga tcgtcgcccc gcaggaaccc ggatcggtgg agagcgagag atccgaggcc
    12421 gtcaccgtcg gtgcctcatc gtcggccacg gtgaccgtct gggtggcggt ctgggtgttc
    12481 ccgtgaacgt ccgtcgcgag ccacgtgatc gtcgtcgctc ccttcgggaa gggatcggtc
    12541 agcgcggcgc cgtcgcctcg cgttccgacg aaggtgtcga cgccacagtt gtcgttggcc
    12601 gaagcgccga gcgatccgat cgtggccgcg cacccgccgg cgtcggccga gagatcgagg
    12661 ttcgagaccg cgatggtcgg aggcgtgtca tcgaccaccg tcacggtctg ggtcccgacg
    12721 gcggtgttcc cgctgggatc ggtcgccgtg taggtgatcg tggtcgtgcc tacggggaag
    12781 aagttgttct ccggaatgcc cgtgctggag atgctcgcgc cgcaattgtc actcgccgac
    12841 gccgagatga gccccgcatc cgccaccctg acgccgcagg ccgtcgcggc cggtccggtg
    12901 gtcaggttca gggcgggggg cgcctggatg gtcgggggct gggtgtcgtt cacgacgaca
    12961 tgctgcagga cggtcgccgt cttgcccgac acgtcggtcg ccacccaggt gaccgtcgtg
    13021 gtgcccttgg cgaacggggc cgtaaggaac gcaccgtcgc ttcgggtgcc gatgaggctc
    13081 acgacaccgc agttgtccgc cgccgatgct cccagagcgc tgaccgtcgc gacacacgtg
    13141 cccggatcgg tggattgcgt gatgtccgcg acctggatcg agggcggctc cacgtccacc
    13201 acggtcaccg tgaactcgca tgcagcgctg gcgcaggagt tcacggcact tacgcagacg
    13261 tgggtcaccc ctacaggaaa gaaggatccc ggatcggcgg agtacgtaat cgcgggtatc
    13321 ggggatcccg tggccgtcgc cggcgggaag ttcacgaccg ccccgcactg cccaggcgca
    13381 ttgttgacga cgacgtcggc gggacactgg aggaccgcgg gggcgccgac ctcgtagagg
    13441 ccgccatcgc cgatggcgaa gtcgttggtg gacgtggccg cgcgcaccgc gaaccccatg
    13501 ggcccgacgg ccgcggagcc gtcggtggag agagtcagcg tcgacgtggc cgaggcctgt
    13561 cccgattgga atcgcacgat ggccgggaag aaggatgccg tgatcccggg atggagatcc
    13621 ccaaagatcg acagcaaggc ggtgaaggaa ccggagctcc ctccgccgct gccgcggttc
    13681 accgtcaccg tgaaggtggc tgagccgtgg ctgccggcgc acagagagcc gttctgcatg
    13741 ccgatcgaga ccgatcccac cttcggcgag tcggtgaaga aggccgtcgc gacgatgccc
    13801 gaggtcaacc cggtggcgac caggtcgaag ctggtgccga gatcctgatc ctccaccgtg
    13861 taatcggcgt tccggaacga cccgatagag tcggccgtcg aatagaacac gtacggatca
    13921 tggagcgtcg ggtgctgatc cagtcgaagg gatacgcgct cgcccggcaa gaatccgctg
    13981 ccggtgatga ccacgacgtc gccggggtaa tagtcgggat tgtcggtggc gacgtcggcg
    14041 gcacgagagg gcgtcgccgt gacgaccagg aagaacgacg cggccgcgaa aatggcggcg
    14101 cgcgcggaaa gcacatgacg acggaatgga tggggcatgg tgagcgcaca ttgcgcacga
    14161 accatgcccg gaacgacacg cttctaagtc attgccattc ttgtatagcc acgtccgcgt
    14221 cctcgggagc aggcggtcgg cttcgggttc ggtccctcag cgccctgggc ggattccctc
    14281 agctccgggc tcatcgttgc cgggcagcgt gcccgccgga ggcacccgat cggggtcgga
    14341 cttccagacg aaataggagt acacgatcgc gcccaccatg ccggtggcca ggatggcgaa
    14401 cgcggcctcc atggcgaccg gggtatggac gattcccgcc gccatgagcg cgatgccgcc
    14461 gaccacgaac acccatcccg cgagccggtg ggtgcgggtc caggaacgct tgctggagag
    14521 cgtccagggg gtgcgcacgc cgacgaacca gttgggacgg atcttcccga gcacgttccc
    14581 catcacgaag aacatcgcgc cgatggcgag ccccgcgaat cggggcatgt ccacgggggt
    14641 gcccttggcc gccagaacca tcaccgtgta cagcagggcc atgagcgcga tggtcgtggc
    14701 ccggatcgtg tagtaggccc ccgcgaagcg ggcgtagttg agacgccccg gatccagccg
    14761 cggcaggaac agcatgatca ggtagagggc gaccgacagg agcggcagga acaggaggcc
    14821 ctcgaaacgg cccccgtacc ggtccacctc cccgctgacg ccccagtgca cggggaagct
    14881 ctcgggcgcg tggggccaga tcagcgccgc ggccacgaac atcccggcga tcagggcgag
    14941 gaggggcagc tccgtcttcc agtgcggttt cacgctcgct tctcctttcg gcccacgccc
    15001 aggagcgaca tcacgcccgc aagggcctct tcgtaggcgg cgaggttgag gctgtacacg
    15061 atcgtggttc cagaccgttc ggaaacgacc agcccggcgt gcttgaggac gttgaagtgc
    15121 cccgacatgg tcgatttcgc gagctcgaaa cgctccgcga tctcccccgc cgtcttgtcg
    15181 ccgcttcgca acaggcgcag gatttcgcgg cgggtcggat cgctcaaggc gcggaagact
    15241 tcgtcgatgc ccatggtccc cctggcggcg gctgccgcgg cattgttcgc tatttcgcca
    15301 accaccgaat atggggccct gcgtcacgtc gcgtcaaggg gtcgtttttg gaatcagcga
    15361 aggggggaat cgccgagggc ggcgccctcg agcaccgccc gaagcacctc ggcaccgacc
    15421 ggcttcacca ggtgaccaat gggaggttgc ggggtccttg ccctagtgcg gcgtccagat
    15481 cctgccggcc tcgacgatct cggacgcgcc gggcaaagaa aggggctcgc gggacttcgt
    15541 ggaaaagacg aggcccttgt agctggtcag gtagcgatgc cctcccatca ggaaaccgcc
    15601 cggcgcgtgc accacgattc cacccgggtt gtggatgagg aggcgctcga attccgacgc
    15661 atcgagtcgt acgatcgcgc ccgatgcttg aatcgcccgc atccgggccg ccgccgcggc
    15721 cgccactgcc ccaccgctca tttcgatttc tcctttcctg gttcttgacg ggctgcctct
    15781 ccgcgggctg ctcgtcgcgg gaggcgcagc atgatacagg gacgctcgcg gccgagcatt
    15841 ccattcccct gacgacggtc gagttcgccg gccaccgtta cgtcgcgcgc gtggatgtgg
    15901 ggttggggga aaccgtacct ctgatgattc acggcaactc gaggatgttc ctctcgctga
    15961 cccacgcgat cggcgccaag gtgaatgggg caccggtgcg ggccacggaa cgctacggct
    16021 acagctcgaa agggagaggc agcctgcgcg tggcgcgagt ccggttgggc ggggccacct
    16081 tccctgagat cccgcccgtg ccggtgttcg acttcaccga gaacggcgac accacggtcc
    16141 aggggatgct cggcgtgccg ttccttgtcg cggcgcgcgc ggccgtcgat ttctctcggg
    16201 atcggctcct gctcggtgtc gccgagcggg ccgaa
//
LOCUS       DASSLZ010000327         4896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1644722, whole genome shotgun sequence.
ACCESSION   DASSLZ010000327 DASSLZ010000000
VERSION     DASSLZ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644722"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..1243
                     /locus_tag="VFM00_12365"
     CDS             446..1243
                     /locus_tag="VFM00_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7499084.1"
                     /translation="MLRLPAITPYGIPRLHVKAAYVEDASGQVLFQKNPERVYPIASL
                     TKLVSALVLLDTTPDWDRLVEVLPEDVRNSSRSHIRPHEEITVRDLIHAMLMSSDNVA
                     AKALARTCGLDRSEFVRRMNVKADSLGLTGARFVEPTGLSEQNVATAQAVAKLLRAAA
                     SNDIVSTIMQKEQYTFTSNRRLHLLINTNRLLRSKWRVTAGKTGFIQEAGYCLATNIQ
                     APTGTEITAVVLGAPSNALRFAEAKRILDWTVRFALHRDLDPAVENQ"
     gene            1246..2799
                     /locus_tag="VFM00_12370"
     CDS             1246..2799
                     /locus_tag="VFM00_12370"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7499085.1"
                     /translation="MPPARRHDEPGRLAAPRFRLRTFALLLTLAGASFPTLAWSAGTE
                     PIAGAFIYPVGDELDFTQAHPGESAGFTISDSYLVVRDGAHGQRVHKGVDLSCGHGGS
                     PVRAVASGVVVVADARAMIRDRSAKNVKVTKVVKGKRVTRWVTRYRTTTRWRTGWGNY
                     VVIRHTLPDGQIVHSLYGHLKSGSIRVRGGETVAAGQVIAQVGNTGRASSPHLHLEIR
                     NAQPDPSAGEDFDPEEAESATVQDRTFALLATVDPVAFLEHHVRRFEDLDPRGWESRY
                     ALAACRDGILAGDESRFEPDEAITRVDFYRALTSLFGIAGTGRAAGKSSGDGAEPFAS
                     LHTALVGAGVLNAVLRSERPGDALTRSEALELLLRCLDKSAARGQNLASFEPPALGSD
                     FNRVFAGADAAALADRQARAAALAETAARRKAADAAYARALRAAKGSHGRSHKRVKRA
                     VVKPVAPTARLDPGVQALAQSDHRLSRAEACLLLASAFRLGHEHASALEQAAARVTAA
                     SSSSEASSR"
     gene            2865..4217
                     /locus_tag="VFM00_12375"
     CDS             2865..4217
                     /locus_tag="VFM00_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7499086.1"
                     /translation="MSTKHPPRRPRPKLVRAEGMRVWDSEGRRYLDATSGAFCMQLGY
                     TRPDLSAAMAEAACSLPHARPSLFDSDAACLYREDLLRAAGPPFTRVLLASSGSEAVE
                     IAMKIAWCWQRARGAADRRSVLSLSGHYHGSTLAALDATGWTERRAPYRQWLTERAFG
                     PAAHCARCFRGLTHPSCGLACADAALDQAETAAALLAETVPAAGLAAAVPPKGWLERI
                     RARCDDAGALWIADEVLTGFGRTGALFAWSRLAERDGVDVAPDLVVFGKGAAAGFYPV
                     SGVLVSARIAEALDSGATPFRHAQTFGGSPVGAAVARRALAAYVSEGIWERVRGAEAA
                     AARAFEPLRACEPVMDVRGIGFLWGVELAGPGPERAPFSRAGAARQVEAACRARGVLI
                     HAASGFLPDGRGDALLVAPPLVAELSDLETIAAALRESIEAAIPSAGRRAWVEEWEDR
                     "
     gene            4214..>4896
                     /locus_tag="VFM00_12380"
     CDS             4214..>4896
                     /locus_tag="VFM00_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499087.1"
                     /translation="MTSWRCAAALALGCVLLLGCASESTLIREDPGFTAPALRSGGLA
                     ILGVVLVDEVPQARPPLVDALQRVLAGARRDIPVVPAARVQAALGDSATRLFLLGYQL
                     RGDPDSSWLARAAESARPLARYGALARVRETRIHYGTRDIPSSQATGMGEASVRVTGR
                     DVQAEVTVYDLTTRAVVYRGKFLGSYDAAPNFRPPPADTLNPDSVEVGMRVPRQGPTF
                     RQPTSFAIPG"
BASE COUNT          668 a         1821 c         1694 g          713 t
ORIGIN      
        1 ccgcataaaa ttgacgcggc gcgcgccacc cgggcgcgcc gcgtttcttc catccggctc
       61 agatgcaacc acccggtaga caggatggca ggggccatgc tattatcttg tccagtaggg
      121 gcttccgacc ccgtcaggcc agaactaccc cttcagttct gcctgccgca tccgataacc
      181 ctgagagagg cgccttcccc gtctctccac caaccgtcat tcagggagta actcacggat
      241 gagggcagaa cgatctatga cttctccgcg ccagatccga aaaatcgccg taatcgcctg
      301 tgtcgcaatt gctttcggcc tcgttgccga ggccagggag gcttccgcgg cagcccggtc
      361 ctcgcgcggt agggcgcgga tcagcgccac ggctgccaga tcgacccgcc atcggccgag
      421 ggtccgccgt cgccgccggg tcgccatgct ccgtctcccg gccataaccc cctatggcat
      481 cccgcggctc cacgtcaaag ccgcctacgt cgaggacgcc agcgggcagg ttctcttcca
      541 gaagaatccc gaacgggtct atccgatcgc cagcctgacc aagctggtca gcgccctggt
      601 gctgctcgac acgacgcccg attgggaccg cctcgtcgag gttctcccgg aagacgttcg
      661 caactccagc cgctcgcaca tccgcccgca cgaagagatc accgtgcgcg acctgatcca
      721 cgccatgctc atgtcatccg acaacgtcgc cgccaaggcg ctcgcgcgca cctgcggtct
      781 cgaccgcagc gagttcgtgc ggcggatgaa cgtgaaggcc gactccctgg gcctcaccgg
      841 cgcgcgcttc gtcgagccca ccggactgag cgagcagaac gtcgctaccg cgcaggccgt
      901 ggccaagctc ctccgcgccg ccgccagcaa cgacatcgtc agcacgatca tgcagaagga
      961 gcagtacacc ttcacgtcca accgccgcct gcacctcctg atcaacacga accggctcct
     1021 gcgcagcaag tggcgcgtca cggccgggaa gaccgggttc atccaggaag cgggctactg
     1081 cctcgccacc aacatccaag caccgaccgg aacggaaatc acggctgtcg tcttgggggc
     1141 gcccagcaac gcccttcggt tcgccgaagc caagcggatt ctcgactgga ccgtccgctt
     1201 cgcgctccat cgcgatctcg accccgccgt ggagaaccaa tagggttgcc gccggcgcgg
     1261 cgacacgatg aaccggggcg cctcgcggcg ccccggttcc gtctccgcac cttcgcgctc
     1321 ctcctcacgc tggcgggcgc gtcgtttccc actctcgcct ggtccgcagg caccgaaccg
     1381 atcgccggcg ccttcatcta ccccgtcggc gacgagctcg atttcaccca ggcccatccg
     1441 ggcgagtccg cgggtttcac catctccgat tcctatctcg tcgtgcgcga cggagcgcac
     1501 ggacagcgcg tccacaaggg cgtggacctc tcgtgcggac atggaggatc gcccgtccgc
     1561 gcggtcgcgt cgggggtcgt cgtggtggcc gatgcccggg cgatgatccg ggaccggagc
     1621 gcgaagaacg tcaaggtgac gaaggtcgtg aaggggaagc gcgtgacccg gtgggtgacg
     1681 cgctaccgga ccacgacgcg gtggcgcacg gggtggggca actacgtcgt gatccgccat
     1741 acgctccccg acggacagat cgtgcactcg ctctacggcc acctgaagtc cggctcgatc
     1801 cgggttcgcg gcggcgagac ggtcgcggcg ggccaggtca tcgcccaggt cggaaatacc
     1861 gggcgcgcct cgtcgccgca tctccacctg gagatccgga acgcccagcc cgacccctcg
     1921 gccggcgagg acttcgatcc cgaagaggcg gagagcgcga cggtgcagga ccggaccttc
     1981 gcgctgctcg ccaccgtcga cccggtggcg ttcctggagc accacgtgcg ccgcttcgag
     2041 gacctggacc cgcgcggatg ggaatcgcgc tacgccctgg cggcgtgccg tgacggcatc
     2101 ctggccggcg acgagagccg cttcgagccc gacgaggcga tcacgcgcgt cgatttctat
     2161 cgggcgctca cctccctgtt cggcatcgcg ggaacaggac gggcggccgg aaagagttcg
     2221 ggagacgggg ccgagccctt cgcctcgctg cacacggcgc tcgtcggggc cggcgtgctg
     2281 aacgcggtcc tgcgctccga acggcccggc gacgcgctga cccgctcgga ggcgctggag
     2341 ctcctgcttc gctgcctcga caagtccgcc gcgcgcggtc agaacctcgc ctcgttcgag
     2401 ccgccggccc ttggctccga cttcaaccgc gtgttcgccg gggctgacgc cgccgcgctg
     2461 gccgaccgcc aggcgcgcgc cgccgcgctc gcggagaccg cggcgcgccg gaaggccgcc
     2521 gacgcggcct acgcgcgagc gctgcgcgcc gcgaaggggt cgcacggccg gtcccacaag
     2581 cgcgtgaagc gggccgtcgt gaagccggtc gcgccgacgg cgcgactcga ccccggcgtc
     2641 caggcgctcg cccagtccga ccatcgtctc tcccgggccg aagcctgcct ccttctcgcc
     2701 tctgccttcc gtctgggaca cgagcacgcc tccgcgctgg agcaggcggc cgcgcgcgtg
     2761 accgccgcct cctcgtcgtc ggaagcctct tcgcgctagc cgcagcgccc aggggccggc
     2821 cgcaagggct ggcgcctggc gccgttcact ggcatcctcc cgaggtgagc acgaagcacc
     2881 ccccgcgcag gccgcggccg aagctggtcc gggccgaagg gatgcgcgtc tgggactccg
     2941 agggacggcg ctacctggac gccacctccg gcgcgttctg catgcagctc ggttatacgc
     3001 gtcccgacct gagcgccgcg atggccgagg cggcctgcag cctgccccac gcccgcccgt
     3061 ccctgttcga cagcgacgcc gcctgcctct atcgggagga tctgctgcgg gccgccgggc
     3121 cgccgttcac ccgcgtcctt ctcgcctctt cgggaagcga ggccgtcgag atcgcgatga
     3181 agatcgcctg gtgctggcag cgcgcgcgcg gcgccgccga ccgacgctcg gtgctttccc
     3241 tgtcggggca ctaccacggc tccacgctcg ccgcgctcga cgcgaccggc tggaccgagc
     3301 ggcgcgcccc ctaccggcag tggcttaccg agcgggcctt cggcccggcc gcccattgcg
     3361 cgcgctgctt ccggggactg acccacccct cctgcggcct ggcctgcgcg gacgccgccc
     3421 tcgaccaggc ggagacggcg gcggcgcttc tggccgaaac ggtccctgct gccgggctcg
     3481 ccgcggcggt gccgcccaag ggatggctgg agcggatccg cgcacggtgc gacgatgccg
     3541 gcgcgctctg gatcgccgac gaggtgctca ccggcttcgg gcggacgggc gctctcttcg
     3601 cgtggtcgcg tctcgcggag cgcgacggag tggatgtcgc tcccgacctc gtcgtcttcg
     3661 gaaaaggagc cgcggcgggg ttctaccccg tgtccggcgt gctcgtctcg gcgcggatcg
     3721 cggaggcgct cgactcagga gcgacgccct tccgccatgc gcagaccttc ggcgggagcc
     3781 cggtgggcgc ggccgtcgcg cggcgcgcgc tggccgcgta cgtatccgag gggatctggg
     3841 agcgcgtccg gggcgcggag gccgccgccg cacgcgcctt cgagccgctg cgcgcgtgcg
     3901 agccggtgat ggacgtgcgc gggatcggat ttctctgggg cgtggagctg gcgggcccgg
     3961 gaccggagcg ggcgcccttt tctcgcgcgg gagcggcgcg gcaggtggag gcggcatgcc
     4021 gcgcgcgcgg ggtcctgatc cacgccgcgt cgggcttcct gccggacggc cggggcgacg
     4081 ccctgctcgt cgcgccgccg ctcgtggccg agctcagcga cctggaaacg atcgcggccg
     4141 cgctccgcga atcgatcgag gcggcgatcc cgagcgcggg acgccgcgcg tgggtcgagg
     4201 aatgggagga tcgatgacat cgtggcgatg cgccgccgct ctggctctgg gatgcgtcct
     4261 gctcctgggc tgcgcctcgg agtcgacgct catccgcgag gatcccggct tcacggcgcc
     4321 ggcgcttcga agcggcggcc tggccattct gggcgtcgtc ctggtggacg aggtgccgca
     4381 ggcgcgcccg ccgctcgtgg acgcgctcca gcgcgtgctc gccggagcgc gccgggacat
     4441 tccggtcgta cccgccgcgc gcgtgcaggc cgcgcttggc gattccgcca cgcggctctt
     4501 cctgctcggc tatcagctgc gcggcgatcc cgattcgtcg tggctcgcgc gggcggccga
     4561 gtccgcccgg cctctggccc gctacggcgc tctcgcgcgg gtgcgcgaga cccgcatcca
     4621 ctacgggacg cgggacattc cctccagcca ggcgaccggg atgggcgagg cgtcggtgcg
     4681 ggtcaccggg cgcgacgtgc aggccgaggt gacggtctac gacctgacga cgcgcgccgt
     4741 ggtctaccgg gggaagttcc tcggcagcta cgacgcggcg ccgaacttcc ggccgcctcc
     4801 cgcggatacg ctgaacccgg actccgtcga agtggggatg cgggtgccgc ggcaggggcc
     4861 gaccttccgc cagccgacga gcttcgcgat acccgg
//
LOCUS       DASSLZ010000328         5186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1381219, whole genome shotgun sequence.
ACCESSION   DASSLZ010000328 DASSLZ010000000
VERSION     DASSLZ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381219"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1009..4056
                     /locus_tag="VFM00_12385"
     CDS             1009..4056
                     /locus_tag="VFM00_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET7499088.1"
                     /translation="MGAGALAEAPGTPRATTLDDVRGLIRAGRYEEAERAARALLAEA
                     ERVYGDESMPVAEILDQLAEAMRNAGHSGAPDALGICERAVRLKEKLAGKSSAPYAAS
                     LHNLGALHLTNGDLSLARPPLERALEIRRKALGEDHPDVARSMIFLAKLDADQARLGD
                     AETLVEGAIAIQQKVLPEDDPERMRALNMLASLKYELGDYVTPASMWEHVLSSRRKTL
                     GADHPLVAEALHNLGTLAVETGDLDEAMTYLEGALRIRRDRLGSYNPYVAFTLLALGQ
                     VQRSAGDRAGARARLEEALRIQERPENKNQINLAWTLGKLGDLLIELKDYTRARALLT
                     RARRIQERGGEGGHLDLAWTLNSLARIAEHDGNWAEAEALYASSLKIKQTLYGDVSPD
                     VVETESQLARCLLAGRDSARAFPIALGAARARAEHLRSTAGGLSERQALAYAGVGSLP
                     LDLLLAMAPAGSKEQVSAAWDALIRSRTIVLDEIAARHGAVMSGLQDSSITGPRRELD
                     AARQRYANLLVRGIGGRDPGPYRAAIDRARDDVERAERVLAARSGRFRTAMRREVVGW
                     KEVGDALPEGWDLVAYALYGEGAQRSYVAFVGRRGQPPTAVRLGTAAAIDPLIQRFVR
                     ACGRSDRGPGGTLDRRAEPACRAAGLALRRRIWDPIQSRLSGAKRLFVVPDGSIHLVS
                     LASLPEGPAYWVETGPLVQYLSAERDLVRLPRRGEATGLLALGGAAFDAPEEEAAGGS
                     ASPDDSVESESTIPARHSVPRGVHRSLGPKPECEEFRNTRFRALPETAREVQEIAALW
                     GDSSRVVLLTGARAGETSLKALAPGRRVLHLATHGFFLDATQCAADERPGVRGIGGVT
                     TERPARSRAASLRTGSPLLLSGLALAGANLRLSARPDQEDGILMAQEVASLDLSNTEW
                     VVLSACDTGLGKLQAGEGVLGLRRAFEAAGAGAVIMSLWEVEDRSSREWMRHLYESRF
                     RLGRPTPEAVRDADLAVLRDRRAQGLGTHPFHWAGFVAAGDWK"
     gene            4063..4512
                     /locus_tag="VFM00_12390"
     CDS             4063..4512
                     /locus_tag="VFM00_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499089.1"
                     /translation="MAAKKDPKNNLKNKSKAEGGWCTDDLFFDEEGCLYVPNKALADA
                     IKSSMDAWKNRLKIYRDPPPKPPTPIEEGRYPAAVLEASAGGDRKVEPTGAGVPRALR
                     RNAESTAGDPETSADVETLALPLDASGSGDAYAGNPANMMCPCAPEP"
     gene            4494..>5186
                     /locus_tag="VFM00_12395"
     CDS             4494..>5186
                     /locus_tag="VFM00_12395"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7499090.1"
                     /translation="MRAGTMIATPVVVACLSSRRLHLILMPTEACNFRCVYCYESFAL
                     KQMKPEVVTGVKRLLTRRAPGLSSLALSWFGGEPLLAPSVIEDILSHARALEGEHPGL
                     TVSSDITTNGYLLSRPMADRLLPLGVSVYQISLDGPRDRHDRMRFRPGGLPTFDRIWE
                     NLIALKARPERFRATLRLHVDRDNLDSVPELLRLLASEFSGDDRFDVFIRAIARLGGP
                     RDAALPVLAEGES"
BASE COUNT          739 a         1786 c         1817 g          844 t
ORIGIN      
        1 cgcgtcgcgg ggccggattc ggtgttcgag gaggtgcttc ggaagtattt cggtggagag
       61 ccggatcccc gcacgaccgc gctcctggcc gcgagcgact ccggcgcggt gggtggcggc
      121 cctccgtgag cgaggctgct cgcaccaggc gctagcgcac ctcgccgggt ggcgccggcg
      181 ctttgccgac caaccggcgt cccctccgac accgccgaca cccctcgccg gcaccacggc
      241 ccgccgattt cgcctccaat gacacgaaat tcatgtctct tgcgcgtttc cctgtgcgga
      301 agcacccggg tctgacgttg tagcagggga aacgggcccg atcggggtca gggcaggacg
      361 tgtgccggtg cgcgcccctt gcccgcgggt cgggctcaac atcgtggggg ttgccggcgg
      421 cgggccgact ggggagttag gctgtcttgg gcggcgaaag ccgaccccgg gacaacgtcg
      481 ccggcgcatg gatggcgccg cggactcccg tgcgccggga gacgtgggcc cggcgtatgc
      541 cgcggcgccg ccatgcgcct tctcctggcg cctctccgtg cgcctttcgt tgacaccccc
      601 ctctccctcg tagttccctc atgtcctggg gcgcatcctg gctggccccg cccggccctc
      661 cggccgcccg ccagccaccg attccaggca gggggagccg tgaatcgaac tgtgccgtgc
      721 ggcctggtgc tgcgcgccct tagtctgcgt cgtcccgcgc ccccggtccg tccgcgttgt
      781 gtgtctgctc tcactccgct ccgtcttgcg accgactcct cgccgtctcc gatttcaccc
      841 gtgtcgatac gttcgccatc gtctcctgga tgtacacgca ccgtttcgcg aaaaccaggt
      901 gccttcgccc tttcgtgtgt gctccttggg gcctttctcg cgttctctct ctcggacgcc
      961 gggatttcct gggcttcggc gggtttcgaa tccgtcgcgg cggcggacat gggcgccggg
     1021 gcgctggcgg aggctcccgg aacacctcgg gccacgacgc tcgacgacgt tcgggggctg
     1081 atccgggcag ggcggtacga agaggccgag cgcgcggcgc gcgcgctcct cgcggaagcg
     1141 gaacgggtct atggagacga atcgatgccg gtggcggaga ttctggacca gctggccgag
     1201 gccatgcgca atgcggggca ttccggcgcg cccgacgcgc tgggaatctg cgagcgagcc
     1261 gtgcggctga aggagaagct ggccgggaag agcagcgcgc cctacgccgc gagtctccac
     1321 aacctcggag cgctccacct gacgaacgga gatctctccc tggcgcggcc tccgctggag
     1381 agggcgctcg agatccgccg gaaggccctc ggcgaggacc accccgacgt cgcacgcagc
     1441 atgatcttcc tcgcgaagct cgacgcggat caggcgcggc tgggggacgc cgagactctg
     1501 gtggagggcg ccatcgcgat ccagcagaag gtccttcccg aggacgatcc ggagcgcatg
     1561 cgcgcgctga acatgctcgc gtcgctcaag tacgagctgg gcgactatgt cacgccggcc
     1621 tccatgtggg agcacgtgct ctcgtcgcgg aggaagacgc tgggcgccga ccatccgctc
     1681 gtcgcggagg ccctgcacaa tctgggaacg ctggccgtcg agacgggcga cctcgacgag
     1741 gcgatgacct atctggaagg ggcgctccgg atccgacgcg accggctggg ctcctacaac
     1801 ccctacgtgg ctttcaccct cctcgccctg ggacaagtac agcgctccgc cggtgatcgt
     1861 gctggggcgc gcgcgcgcct cgaggaggcg cttcggatcc aggaacggcc ggagaacaag
     1921 aaccagatca atctcgcctg gacgctcggg aagctgggcg atcttctcat cgagctcaag
     1981 gactacacgc gcgctcgcgc cttgctcaca cgggcgagac gcattcagga gcggggggga
     2041 gagggcgggc atctcgatct cgcctggacc ctgaactccc tggcgcggat cgccgagcac
     2101 gacggaaatt gggccgaggc cgaggccttg tacgcgagct ccctcaagat caagcagacc
     2161 ctctacggag acgtatcccc cgacgtcgtg gagacggaga gccagctcgc gcgatgcctt
     2221 ctggccggcc gtgacagcgc ccgcgcgttt ccgatcgcgc tcggggccgc gcgggcacgg
     2281 gccgagcacc tgcggtcgac ggctggcggc ctgtccgagc gccaggcgct tgcctacgcc
     2341 ggtgtcgggt ccttgcccct cgatctgctc ctcgcgatgg cgcccgccgg ctccaaggag
     2401 caggtctcgg cggcctggga tgcgctgatc cgttcgcgca ccatcgttct cgatgagatc
     2461 gccgcgcggc acggtgccgt gatgtccggt cttcaagaca gttccatcac cggtccccgg
     2521 cgcgagctcg acgccgcacg ccagcgctac gcgaacctgc tcgtgcgcgg gatcggcggg
     2581 cgggatcccg gtccctaccg tgccgccatc gatcgggctc gggacgacgt ggagcgcgcg
     2641 gagcgggtgc tcgcggcgcg gagcggccga ttccggacgg cgatgcggcg cgaggtggtc
     2701 ggatggaagg aggtcggaga cgcgctcccc gaagggtggg accttgtcgc gtatgccctc
     2761 tatggggagg gagcacagcg ctcctatgtc gctttcgtcg ggcgccgagg ccagccgccg
     2821 accgccgtcc ggctgggcac ggccgctgcg atcgacccgc tgattcagcg tttcgtccgg
     2881 gcctgcgggc gatcggaccg tggcccgggt gggacgctcg atcgtcgagc ggaacccgcc
     2941 tgccgcgccg cggggctcgc gctgcggcgc aggatctggg atccgatcca gagccgtctg
     3001 tccggcgcca agcgcctgtt cgtcgtgccg gatgggtcga tccatctcgt cagcctcgcc
     3061 tccctccccg aggggccggc ctactgggtc gagacgggcc cgctggtgca gtacctctcg
     3121 gccgagcggg atctggttcg cttaccgcgg cgtggcgaag cgacgggcct cctggccctc
     3181 ggcggggccg ctttcgacgc tccggaagaa gaggccgccg gcggctccgc gtcgccggat
     3241 gattccgtgg agagcgaatc cacaattccg gcccggcatt cggtgccgcg aggggtccac
     3301 cgctctctcg gtcccaagcc cgagtgcgaa gagttccgaa acacccgctt ccgcgcgctg
     3361 cccgagacgg cccgggaggt tcaagagatc gccgcgctat ggggcgactc ttcgcgcgtc
     3421 gtcctgctga cgggagcacg cgccggcgaa acctccttga aggcgcttgc gcccggccgc
     3481 cgcgtgctcc acctggccac gcacggcttc ttcctcgacg ccacccaatg cgcggccgac
     3541 gaacgccccg gcgtgcgcgg tatcggtggc gtgacgacgg agcgtccggc tcggtccagg
     3601 gccgcgagcc tgcgcacggg aagtccgctt ctgctttcgg ggctcgctct ggccggtgcg
     3661 aacctgcgcc tgagtgccag gccggatcag gaggatggga ttctcatggc ccaggaggtc
     3721 gcgtctctgg acctttccaa cacggagtgg gtcgtcctct ccgcttgcga caccggtctc
     3781 ggaaaactcc aggcggggga aggtgtcctg ggcttgcggc gggcgttcga agctgccggc
     3841 gcgggcgccg tgatcatgag tctctgggag gtggaggacc gctcctcccg cgagtggatg
     3901 cgtcatctgt acgaatcgag atttcgtctc ggacggccta ctcccgaggc cgtccgcgac
     3961 gcggatctcg cggtgctacg ggaccgcagg gcccagggtc tcggcaccca tccgttccac
     4021 tgggccggat tcgtcgccgc gggcgactgg aagtgagggg atatggccgc caagaaggat
     4081 cccaagaaca atctcaagaa caagtccaag gcggaaggtg gctggtgcac ggacgacctc
     4141 ttcttcgacg aggaggggtg cctgtacgtg ccgaacaagg cgctcgcgga cgccatcaag
     4201 agctcgatgg acgcctggaa gaatcgcctg aagatctacc gcgatccccc tccgaagccg
     4261 ccaacgccga tcgaagaggg tcggtatccg gccgcggtgc tcgaagcgag tgcgggcggt
     4321 gatcgcaagg tcgagcccac cggggcgggg gttccgcggg cattgaggag gaacgccgaa
     4381 tccacggcgg gtgatcccga gacctccgcg gacgtggaga cgctcgcgct accgctggat
     4441 gccagcggat cgggggacgc ctacgcgggg aacccggcca acatgatgtg cccgtgcgcg
     4501 ccggaaccat gatcgccact ccggtcgtcg tcgcgtgcct gagctcgcgg cggcttcatc
     4561 tcatcctgat gccgacggag gcgtgcaact tccgctgcgt gtactgctac gagtcgttcg
     4621 cgctgaagca gatgaaaccg gaagtcgtca ccggcgtgaa gcggctcctc acccggaggg
     4681 cgccggggct gagctcgctc gctctctcct ggttcggcgg ggagccgctg ctcgcgccgt
     4741 cggtcatcga ggacatcctg tcgcacgccc gcgcgctcga gggtgagcat cccggtctca
     4801 ccgtgagctc cgacatcacg acgaacgggt atctcctctc ccggcccatg gcggatcggc
     4861 tgctcccact cggcgtgagc gtctatcaga tctcgctcga cggtccccgg gaccggcacg
     4921 accggatgcg cttccgcccg ggcgggctgc ccacgttcga tcgcatctgg gaaaacctga
     4981 tcgcgctcaa ggcgcggccc gagcgctttc gcgccacgct tcgattgcac gtggaccggg
     5041 acaacctcga ctccgtgccc gagctccttc ggctcctggc atcggagttc tccggtgacg
     5101 atcggttcga cgtcttcatt cgggcgatcg cccgcctggg cggtccccgc gatgccgcgc
     5161 tgccggtgct ggccgaaggg gaatcc
//
LOCUS       DASSLZ010000329         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_872688, whole genome shotgun sequence.
ACCESSION   DASSLZ010000329 DASSLZ010000000
VERSION     DASSLZ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872688"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1843
                     /gene="glmS"
                     /locus_tag="VFM00_12400"
     CDS             14..1843
                     /gene="glmS"
                     /locus_tag="VFM00_12400"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET7499091.1"
                     /translation="MCGIVGYVGDQESLPILLEGLRRLEYRGYDSAGVAILNGGGIKI
                     QKSAGKISVLEGLLAENPSHGTLGIAHTRWATHGEPNTKNAHPHTDCTGKIAVAHNGI
                     IENYATLKRALEGEGHTFTTDTDTEVIAHLIEKFYQGNLEKAVAQALRLLTGTYGIAA
                     ICSDEPRKVVGARHGSPLVVGVCDNHQYILASDVSAVIRHTNQVVYLDDEEMVVLTPD
                     GVQTTTIGEVAVSKKIETVDWDLEQIEKAGYDHFMLKEIFEQPQSLRNGLRGRLMVAE
                     GTARLGGLNMSERELRQIERVVILACGTSWHSGLIGEYLIEELARIPVEVEYASEFRY
                     RNPILRPGTVVLAISQSGETADTLAAMREAKRKGARVLGICNVVGSTIARESDGGVYI
                     HAGPEIGVASTKAFTSQVAALALLTLYLGRLGELSPEVGAELTRELHTIPDKMERILA
                     NADAIRSIAKAYAHHNNFLYLGRGVNFPVALEGALKLKEISYIHAEGYPAAEMKHGPI
                     ALIDANMPVVFICTQDSAYEKVLSNMEEVRARKGKIIAVACEGDEVVAQKADHVLFVP
                     HALGPLMPLLSVIPLQLLAYYIAVERGCDVDQPRNLAKSVTVE"
     gene            1845..2240
                     /locus_tag="VFM00_12405"
     CDS             1845..2240
                     /locus_tag="VFM00_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014683547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7499092.1"
                     /translation="MSHGVLKTNDAPAAIGPYVQGRSGNLSGRWIITSGQIGLDPKTG
                     NMVGGGVAAETERVLANLRAVLESGGASFADVVKTTVYLADMDEFAAMNEVYARAFSD
                     PMPARSTVAVKRLPKDARVEIEVWAFLTS"
     gene            2257..3312
                     /gene="selD"
                     /locus_tag="VFM00_12410"
     CDS             2257..3312
                     /gene="selD"
                     /locus_tag="VFM00_12410"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2314..2316,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HET7499093.1"
                     /translation="MSHTREDIHRLTEQVSCAGUAAKLGPEDLRQALSSLPKAKKIAG
                     VLVGPETWDDAGVFRLSPTRALVQTVDFITPVVNDPFDFGRIAAANAMSDVYAMGGTP
                     LTALSIVCYPEKGDMGVLGEILRGGAEALARARVALVGGHTVRDPEIKFGFAVTGEVH
                     PKRIVTNAGAKRGDLLVLTKPLGIGILATALKRRLLPDEDLARMTAQLVTVNRAAGET
                     MHAAGASAATDVTGFGLLGHALNVARASRKTLRIWSGAVPVLPRVLDFAREGVAPAGL
                     RSNLAYASPFVQFDEGVSEPLRMALADPQTSGGLLIAIAPKKAAALMKALKRAKVDAA
                     VVGETLTKGKAPIEVGG"
     gene            complement(4312..>4771)
                     /locus_tag="VFM00_12415"
     CDS             complement(4312..>4771)
                     /locus_tag="VFM00_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499094.1"
                     /translation="ETEIALPRSMKDALARLDPAFVPWSENDRRRMGERVRGPSVITG
                     DFDGDLLQDVALLGRSGADQVVIALLSNLGAVTAREVVWRKVRIGTGESRSRADPKEI
                     PPVYLESVPRGTVNSFCWVVGPTDAIGIVQPGLARFDYVFQENHFVLGSP"
BASE COUNT          810 a         1570 c         1620 g          771 t
ORIGIN      
        1 aaggggttcc ttcatgtgcg gcatcgtcgg gtacgtcgga gatcaggagt cgcttcccat
       61 cctgctcgag gggcttcgcc gcctcgagta ccgcggatac gattccgcgg gcgtcgccat
      121 cctgaacggc ggcggaatca agatccagaa atcggcgggc aagatcagcg tgctcgaggg
      181 acttctcgct gagaacccgt cgcacggcac gctcggcatc gcgcacacgc gctgggccac
      241 gcacggcgag cccaacacca agaacgccca cccccacacc gactgcacgg gcaagatcgc
      301 ggtggcccac aacgggatca tcgagaacta cgccacgctg aagcgcgcgt tggaggggga
      361 ggggcacacg ttcaccaccg acaccgacac cgaggtcatc gcccacctga tcgagaagtt
      421 ctatcagggc aacctcgaga aggcggtcgc gcaggcgctt cggctcctga ccggcacgta
      481 cggcatcgcg gcgatctgct ccgacgagcc gcgcaaggtc gtcggcgcgc gccatgggag
      541 cccgctcgtc gtcggggtct gcgacaacca ccagtacatc ctcgcctccg acgtctccgc
      601 ggtcatccgg cacacgaatc aggtggtcta cctggacgac gaggagatgg tggtcctgac
      661 cccggacggg gtccagacca cgaccatcgg cgaggtcgcg gtgtcgaaga agatcgagac
      721 ggtcgactgg gatctggagc agatcgagaa ggccggctac gaccacttca tgctgaagga
      781 gatcttcgag cagccgcagt cgctccggaa cggcctgcgc ggccggctca tggtcgccga
      841 gggaaccgcg cggctcggcg gtctcaacat gagcgagcgc gagctgcgcc agatcgagcg
      901 cgtggtcatc ctggcgtgcg gcacctcctg gcattccggg ctgatcggcg agtacctgat
      961 cgaagagctg gcgcggatcc cggtcgaggt ggagtacgcc tccgagttcc gctaccggaa
     1021 tccgatcctg cgccccggca ccgtcgtgct ggcgatcagc cagtcggggg agaccgcgga
     1081 tacgctggcg gcgatgcgcg aggccaagcg gaagggcgcg cgggtgctgg gcatctgcaa
     1141 cgtggtcggt tcgacgatcg cgcgcgaatc ggatggcggc gtctacatcc atgccggccc
     1201 cgagatcggc gtggcgtcga ccaaggcgtt cacgtcccag gtcgcggcgc tcgcgctctt
     1261 gacgctctat ctcgggcgcc tcggcgagct ctcgcccgag gtgggcgccg agctcacgcg
     1321 ggagctgcac accattcccg acaagatgga gcggatcctg gccaacgccg acgcgatccg
     1381 ctcgatcgcc aaggcgtacg cgcaccacaa caacttcctc tacctcggcc gcggcgtgaa
     1441 cttcccggtc gcgctcgagg gcgcgctcaa gctcaaggag atctcctaca tccacgcgga
     1501 agggtatccc gcggccgaga tgaagcacgg cccgatcgcg ctgatcgacg ctaatatgcc
     1561 cgtcgtcttc atctgcacgc aggactccgc gtacgaaaaa gtgttgagca atatggaaga
     1621 ggtccgggcg cggaaaggga agatcatcgc ggtggcgtgc gaaggggacg aggtcgtggc
     1681 gcagaaggcc gatcacgtgc tcttcgtgcc gcacgcgctc gggccgctga tgccgctcct
     1741 ctcggtcatc ccgctccagc tcctcgccta ctacatcgcg gtggagcgcg gctgcgacgt
     1801 ggaccagccg cgcaacctgg ccaagagcgt cacggtcgaa tagcttgagc cacggcgtcc
     1861 tcaagaccaa cgacgcaccg gccgcgatcg gtccctacgt ccagggccgc tccggcaatc
     1921 tctccggccg ctggatcatc acctccggac agatcggcct cgaccccaag accgggaaca
     1981 tggtcggcgg cggcgtcgcc gccgagaccg agcgggtgct cgccaacctg cgcgccgtcc
     2041 tcgaatcggg cggggcctcc ttcgccgacg tcgtcaagac gacggtctat ctcgccgaca
     2101 tggacgagtt cgccgcgatg aacgaggtct atgcgcgcgc cttctccgat cccatgcccg
     2161 cccgctccac ggtcgccgtg aaacggctgc cgaaggacgc ccgcgtcgaa atcgaggtct
     2221 gggccttcct cacctcgtga ccggcctccc caacccgtga gtcatacccg cgaggatatt
     2281 caccggctca cggagcaggt gtcctgcgcg ggttgagccg ccaagctggg tccggaggac
     2341 ctgcgacagg ctctgtcgtc cttgcctaaa gcgaagaaga tcgccggcgt tctcgtcggt
     2401 cccgagacct gggacgacgc gggcgtattc cggctgtcgc ccacgcgcgc cctcgtccag
     2461 accgtggatt tcatcacgcc ggtcgtgaac gatccgttcg atttcggccg gatcgccgcg
     2521 gccaacgcga tgagcgacgt ctacgccatg ggcggaacac ccctcaccgc gctctccatc
     2581 gtctgctatc ccgagaaggg agacatgggg gtgctggggg agatcctgcg cggcggcgcc
     2641 gaagcgctgg cgcgcgctcg cgtcgcgctc gtgggcgggc acaccgtgcg cgatccggag
     2701 atcaagttcg ggttcgcggt cacgggtgaa gtgcatccga agcggatcgt gacgaacgcg
     2761 ggcgccaagc gcggcgacct gctcgtcctg accaagccgc tcgggatcgg catcctcgcg
     2821 acggcgctca agcggcggct gttgcccgac gaggacctcg cgcggatgac cgcgcagctc
     2881 gtcaccgtga atcgcgccgc gggggaaacg atgcacgccg ccggcgcgtc cgccgcgacc
     2941 gacgtgacgg ggttcgggct cctgggccat gcgctcaatg tcgcgcgggc cagcaggaag
     3001 acgttgcgga tctggagcgg cgcggtgccg gtgttgccgc gggtcctgga cttcgcgagg
     3061 gagggcgtgg cgccggcggg gctgcggtcg aacctggctt atgcctcacc gttcgtgcag
     3121 ttcgacgaag gcgtgtccga acccctgcgc atggcgctcg ccgatccgca gacgagcggc
     3181 gggttgctga tcgcgatcgc gcccaagaag gccgcggcgc tgatgaaggc gctcaagcgc
     3241 gccaaggtgg atgcggcagt ggtcggggag acactgacaa agggtaaggc gccgatcgaa
     3301 gtcgggggat gaaggtgatc gttcgccagg cttcctggat cgtcatggcg ctgtgtctga
     3361 ctggcgcggt ggcgcgtgcc gaggctccgc cggactcctt ggcgcttccc gagaccgcat
     3421 cggactcctc ggctgtggcc caaggctcga ttcgagttct gctggacgac ggaaccgagg
     3481 tgcgggcaca gcgtgttcga atcggttctc tgggaagcct tggattgatt ctcgagaacg
     3541 ggcgggactc ggtcctggcc gcgagtcgag tgcgccggat cgaggatgaa aagggccgtg
     3601 accggacgca cgaggttctc gtcgacgagc ggagcatccg tgtcggcggg ccggctcccc
     3661 cgaagcggga cgatccgcca ccgaatgtcg cgcgctggat cgtgagcgct cacgggagcg
     3721 cggcgaaccc ggtgggcgcc tttggccact ctgccgcgaa cggctatgcg gtcgatgggg
     3781 tcatcgaacg acgcacccgg cgtcacgaaa gctacgggct ccgggtcgat ttcgcggagt
     3841 tcgggggcga tacggggttc gaggagctac tggctcgaac ctatcccggt ggccccgacc
     3901 agcttcacta tcgattcctc ggtgtgagca tctcctcgca ctccttcatc ctgccggatc
     3961 gcaggctgaa tccattcatc ctgaccacac tcggagtggg acgtctcagc gtctcgttgt
     4021 cgggccccgc tgaagaggcg acatcctcag gcatctcgac caccggcgag gccggcctcg
     4081 gactggcgat tcgtcttgga gaacgcctgg atgcggagct cctgggatcg tatgggcgta
     4141 ccggttcggg ccggagatac ttgaggacgg aaagcgtgag catgagcgcc gatggggtcg
     4201 cgcagtacct tcggttcacg gcggggctcg tccaccgatt cggtccgggc cagaagacgt
     4261 catcggagtc gtcggcaccc gcagggattg cgtcccgacc cgtcgctctc ttcaagggga
     4321 tccgagaacg aaatggttct cttgaaagac gtaatcgaaa cgagctaggc ccggctggac
     4381 gatgccaatc gcgtccgtgg ggccgaccac ccagcagaag ctgttcacgg ttcctcgcgg
     4441 caccgactcc aggtacaccg gcgggatttc cttggggtcc gcacgcgacc gactctctcc
     4501 cgttccgatc cgtacctttc tccagacgac ctccctggcc gtcaccgcgc ccaagttcga
     4561 gagcagggcg atcaccacct gatcggctcc gctccttccg aggagtgcca cgtcctggag
     4621 gagatcgccg tcgaaatcac cggtgatgac ggaaggtcct cgtacgcgct cgcccatgcg
     4681 gcgccggtcg ttctcgctcc aggggacgaa ggcgggatcg aggcgggcga gggcatcctt
     4741 catcgacctg ggcagcgcga tttcggtctc a
//
LOCUS       DASSLZ010000330        10936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_116814, whole genome shotgun sequence.
ACCESSION   DASSLZ010000330 DASSLZ010000000
VERSION     DASSLZ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116814"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..532)
                     /locus_tag="VFM00_12420"
     CDS             complement(44..532)
                     /locus_tag="VFM00_12420"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7499095.1"
                     /translation="MPLLLARVDDRLVHGQVAHGWGRALRPTLYLVVSDELADDPARG
                     DLYLLAVPEDATGRCVSVREALAPETRALVERERAVMLFPGLDEPLRLVEGGFPISDL
                     NVGGLHHAPGKAPVLPYVFLDEPDRERLRRLASLGVRVAARDLPSNPEHPLEELLPGS
                     AS"
     gene            complement(532..936)
                     /locus_tag="VFM00_12425"
     CDS             complement(532..936)
                     /locus_tag="VFM00_12425"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499096.1"
                     /translation="MNGSAGGARPVAILVTHGVLGEELVRTVEAILGAQSAVITVSNS
                     GLSADGLRDRIDAEIAGLPEGVPVVLFSDLAAGSCGIATRRADPAARTVKRIAGVNLP
                     MLLEFFYNRDAIPLEQLLARMEAKGKAGIVLY"
     gene            complement(941..1924)
                     /gene="hprK"
                     /locus_tag="VFM00_12430"
     CDS             complement(941..1924)
                     /gene="hprK"
                     /locus_tag="VFM00_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HET7499097.1"
                     /translation="MSSTSPPTLTVAQLYERQKEQLSLELLSDSSDSKIPITMSDINR
                     PGMALMGFTENFLAERIQIFGRTEIAYFQTLGPKEQTEALDRFFQFRVPCIIVAKGLD
                     LPGGLLDRANREAVPVFRTPQDTTPFIHQLTAYLDFVFAPHVTTHGSLVDVYGVGLLF
                     TGRSGIGKSETALDLVERGHRLVADDVVRITRRHGDILIGSCNEVLKHTMEIRGLGII
                     DVQSIFGIRSIRGQKRVEVEVHLTDWSEARDYERLGMDEQTVKHLDVEIPLVTIPIFP
                     GKNITVIAEVVALNHLVKVYGYHPARALNDRLLESMRMKVLSSPLVRDDTE"
     gene            complement(1911..2450)
                     /gene="raiA"
                     /locus_tag="VFM00_12435"
     CDS             complement(1911..2450)
                     /gene="raiA"
                     /locus_tag="VFM00_12435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET7499098.1"
                     /translation="MDITIKGRHWKPTPAFRESAEARIQKLARFYPSLIRAELVVTKE
                     GFRHIAEIRLHGNSVDLLTRGTDQDPLVALDQVLAKQEKALSRHKDRLKDKKKRAPAM
                     RAEPAELETLRLPSTRPAGISVVRQKTKAPLLSAEQAARALLKGNKPVLVFSERGTNS
                     GLRVVYRLGDREVALLELD"
     gene            complement(2479..3912)
                     /gene="rpoN"
                     /locus_tag="VFM00_12440"
     CDS             complement(2479..3912)
                     /gene="rpoN"
                     /locus_tag="VFM00_12440"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET7499099.1"
                     /translation="MEMKAGLHQQMRQTLIITQRLQQALKLLQMPTLELQQALKAELE
                     RNPMLEEVDEVEEVQEIDDAKEETGLKESEQPEAPDATPEDQKVEWEDFWPDSSWDGP
                     VAPRTDDPGEEFYEKVPVTVKSLSEHLSEQLRLTGMGELELSIGDYLIGSIDENGYLQ
                     TTVDEVAEALAAPPEKVEEVLAIIQTFEPAGVGARNLQECLWIQLAQKNLQATLAGRI
                     VQEQFDNLLNKRFSEIARAMRCTVEEVQAAGDLIATLDPRPGQEVAAEETRYVTPDLV
                     VERVGEDYVVSLNDRNVPRLRINNAYQHILRKKSGGDEERKFISEKLNSAKWLIQTIE
                     QRRKTMIKVMRRIVEEQRDFFEKGVEGLRPLTLQQIANQIGMHESTVSRVTTNKYVQT
                     PRGVFELKYFFSSGLATEEGDDVSAKAAKDKILQLINGEDKLDPMSDQRIAEVLQESG
                     LKIARRTVAKYREALRILPARARRRYA"
     gene            complement(3925..4641)
                     /gene="lptB"
                     /locus_tag="VFM00_12445"
     CDS             complement(3925..4641)
                     /gene="lptB"
                     /locus_tag="VFM00_12445"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HET7499100.1"
                     /translation="MVARDLERFYGQWKVVDKVSITVDQGEVVGLLGPNGAGKTTTFY
                     LIVGLLRADAGQILVDGRDITRMPMHRRARLGIGYLAQEPSIFRRLTVRENILAILET
                     LPLSRKERASRLAQLLEELNIAHLADRKGYNLSGGERRRVEITRALVTEPKFMLLDEP
                     FVGIDPIAVAEIQDIVGRLQAKGLGILITDHNVRETLSTTDRAYIMFEGRILLQGTSR
                     ELADDPVARQIYLGERFRLD"
     gene            complement(4647..5249)
                     /gene="lptC"
                     /locus_tag="VFM00_12450"
     CDS             complement(4647..5249)
                     /gene="lptC"
                     /locus_tag="VFM00_12450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HET7499101.1"
                     /translation="MVESRSGPPAGPRRSRVLARAPVLAALAAVLTAFGCQSRPASTP
                     SGEDVRLPDQEARDFTLTESSEGKKNWTLWASYAAMYNDQSLVDAKTVRIEFFDKEGK
                     KFSTLVAKTGRVHQRTNDLEARGNVVVTTESGIRMETDSLRWQNTAGKIVSDGFVKVT
                     RQHDVVTGYGFESDPSLDHFHLRREVRAEVTDTEGNVDSL"
     gene            complement(5290..6291)
                     /locus_tag="VFM00_12455"
     CDS             complement(5290..6291)
                     /locus_tag="VFM00_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HET7499102.1"
                     /translation="MTLAAETGTASKVVAWAREVLDLEGAAVLQLKPRIGNEFEAAVQ
                     ALSKARGQILTLGVGKSGLVAKKIAATLTSTGSPATFVHPVDAVHGDLGIVATDDAAL
                     LLSKSGETRELIDLVPAFRRRGVCVVAMTASRESSLAAASDHVLEIGAPREACGEDLV
                     PTSSTTAMLAMGDALAVVLLRLKGFTREDFAVLHPGGVLGHAASYRVCDLMHQGAALP
                     RVDEGATLRQALLEILNKRLGMTTVVDGAGKLAGVLTDGDLKRILLRGGGDLAQPVSA
                     VMSRTPKTIEPEATIARAVRVMEENPGGPITSLVVGDESGTPVGVLHLHDCLGVRAG"
     gene            complement(6312..7139)
                     /gene="kdsA"
                     /locus_tag="VFM00_12460"
     CDS             complement(6312..7139)
                     /gene="kdsA"
                     /locus_tag="VFM00_12460"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HET7499103.1"
                     /translation="MRVGPVAFGPEQLGLIAGPCALEDEGEAMEIAREVKRIAGSLGM
                     PFVFKASYAKANRTSGDSYRGPGPSEGLRSLARIREAADVPVTSDVHESTEVGDAAAV
                     LDLLQIPAFLCRQTPLIEAAAQTGKPLHVKKGQFLSPSGMRHVVDKARAAGAAGVILT
                     ERGTTFGHGDLVVDFRGLPTMRALGCPVFFDATHSVQRPAGVETGGDRAMIPVLARAA
                     VAAGVDGIFIETHPAPERARSDRESQWPLAELEELLRSLMKIRQAVADTVVLEGQRA"
     gene            complement(7166..8830)
                     /locus_tag="VFM00_12465"
     CDS             complement(7166..8830)
                     /locus_tag="VFM00_12465"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET7499104.1"
                     /translation="MTKFVFVTGGVVSSLGKGIAASAIGSLLKSRGLAVTLQKFDPYL
                     NVDPGTMSPYQHGEVYVTDDGAETDLDVGHYERFLGVSMGRENNVTAGQIYDAIIQKE
                     RRGDYLGRTVQVIPHVTDEIKARMMALTRGRPVDVAIVEIGGTVGDIESLPFLEAIRQ
                     LRLELSSLNTLFIHVTLVPHLGAAREIKTKPTQHSVKELRAIGIQPDILLCRTEVPLP
                     QEVKEKIGLFCNVPTRSVIEAIDVPSIYEVPVMFHKGGLDDLVVQMLRLDAGPADLRV
                     WNAFSERMRSARESVTVSVVGKYTHLRDAYKSIQEAIQHAGVSNGVTVKIDWVDSERV
                     ELDGPEALLGQTHGILIPGGFGDRGTEGMVQAARYARERKVPFFGICLGMQCAVIEFA
                     RDVAGLKGADSSEFDPSTPHAVIDLMPEQEGVSQMGGTMRLGKYEAALAAGSHAQAEY
                     GRPRVEERHRHRYEFNNAYRDVFQERGMRVTGLFEARDLVEIVELPDHPWFVGVQFHP
                     ELKSRPSDPHPLFRGFIRAANEERHRREAGAPARPGQESASGAPRP"
     gene            complement(8850..9701)
                     /gene="kdsB"
                     /locus_tag="VFM00_12470"
     CDS             complement(8850..9701)
                     /gene="kdsB"
                     /locus_tag="VFM00_12470"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HET7499105.1"
                     /translation="MPTRPTSGAVAIIPARLQSTRLPEKPLQDLGGKPLVVRVLERAR
                     RATSLEAVWVATDSERIVKVVGDAGGETILTRADHPAGLDRVAEAAIALCEMTRRGSQ
                     ADGVRDPDLAADPVILNVQGDEPFLNPAGLDHLVSLFDRPEVRMATLAAPFAAGDDPA
                     DPNRVKVVLDRMGRALYFSRSLIPHGAGGAPPGGAQTGAAQTGAAATTPLLHVGIYAY
                     RLTTLRELAALPPAPLETCERLEQLRALWNGIPIHVAVGDYHSIGIDTPEDLARARTR
                     WSEGGAS"
     gene            complement(9788..10477)
                     /locus_tag="VFM00_12475"
     CDS             complement(9788..10477)
                     /locus_tag="VFM00_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET7499106.1"
                     /translation="MFVGHFAVAFAGKRVAPKMSLGTLIFGAQFLDLIWPPLVLLGLE
                     RFHIDVNSTKATPLAFDSYPISHSLLMAILWSVLIGGVYWARKRYRTGALFLGAAVFS
                     HWFLDWITHRPDLQLVPGIATRVGLGLWNHPALEVTIETAMYVAAIGIYAVQTRARDR
                     RGTWGFVLFLAFLSLIWLGDVAGPPPTPGPEMVRTIAWSGLTLWLLVPWAAWFDRHRV
                     PRRSTEAAPQT"
BASE COUNT         1621 a         3829 c         3693 g         1793 t
ORIGIN      
        1 gaccacggcg cctccccggc gcgcgcgccg cgcgcgcgcg ccgtcatgaa gcgctcccgg
       61 gcaggagctc ttcgagcggg tgctcgggat tgctgggcag gtcccgcgcc gcgacccgca
      121 ccccgagcga ggccaggcga cggagccgct cgcgatccgg ttcgtccagg aacacgtacg
      181 ggagcaccgg cgccttcccc ggcgcgtggt gcagcccgcc gacgttgagg tcggaaatcg
      241 gaaagcctcc ctcgaccagc cgcagcggct cgtcgagccc cggaaagagc atcaccgccc
      301 gctcgcgttc cacgagggcg cgcgtctccg gagcgagcgc ctcgcgtacc gagacgcagc
      361 ggccggtggc gtcctcgggc acggcgagca gatagagatc gccgcgggcg ggatcgtccg
      421 cgagctcgtc cgagacgacg aggtagaggg tggggcgaag cgcacggccc cagccgtggg
      481 cgacctgccc gtggaccagc cggtcgtcga cgcgcgcgag gagcagcggc actagtagag
      541 gacgatcccc gccttcccct tggcttccat gcgcgcgagg agctgctcca aggggatggc
      601 gtcgcggttg tagaagaact cgaggagcat cggaaggttc acgccggcga tccgcttcac
      661 cgtccgcgcg gcgggatcgg cgcggcgggt ggcgatgccg cagcttcccg cggcgagatc
      721 gctgaacagg acgacgggca ccccctcggg gaggccggcg atctccgcgt cgatccggtc
      781 gcggaggccg tcggccgaga gcccgctgtt gctgaccgtg atcaccgccg attgcgcgcc
      841 gaggatggcc tcgacggtcc gcaccagctc ttcgcccagc acgccgtgcg tgaccaggat
      901 cgcgaccggt cgcgcgcctc cggcggaccc gttcatgcgg ctattccgtg tcgtcccgga
      961 cgagcgggct ggagaggacc ttcatgcgca tcgattcgag cagccggtcg ttcagcgccc
     1021 gcgcggggtg gtagccgtag accttgacca ggtggttcag cgcgacgacc tccgcgatca
     1081 ccgtgatgtt cttccccgga aagatgggaa tggtgaccag cgggatctcg acgtcgagat
     1141 gcttcaccgt ctgctcgtcc attccgaggc gctcgtagtc gcgcgcctcg ctccagtcgg
     1201 tcaggtggac ctccacctcg acgcgcttct gcccgcggat cgagcggatg ccgaagatcg
     1261 actggacgtc gatgatgccg aggccccgga tctccatcgt gtgcttcagc acttcgttgc
     1321 acgacccgat caggatgtcg ccgtggcgcc gcgtgatccg gaccacgtcg tcggcgacca
     1381 ggcggtggcc gcgctccacc aggtcgagcg cggtctcgct cttgccaatg ccgctccgtc
     1441 cggtgaagag cagccccacg ccgtagacgt ccacgaggga gccgtgcgtc gtgacgtgcg
     1501 gggcgaagac gaagtcgaga taagccgtga gctgatggat gaagggggtg gtgtcctgcg
     1561 gcgtgcggaa gaccgggacc gcttcccggt tcgcccggtc gaggaggccg ccgggaaggt
     1621 ccagcccctt cgccacgatg atgcagggga cgcggaactg gaagaaacgg tccagcgcct
     1681 cggtctgctc tttcggcccc agcgtctgga agtacgcgat ctcggtgcgg ccgaagatct
     1741 ggatgcgttc ggccaggaag ttctcggtga agcccatgag cgccatcccg ggccggttga
     1801 tgtcgctcat ggtgatcgga atcttcgaat cgctggagtc cgacaggagc tccaggctca
     1861 gctgttcctt ctggcgctcg tagagctgcg cgaccgtgag ggtcggcgga ctagtcgagc
     1921 tcaaggagcg cgacctcccg gtccccgaga cgatagacga cgcgcagacc cgagttcgtc
     1981 ccgcgctccg agaacacgag caccggcttg ttccccttga ggagcgcgcg ggcggcctgc
     2041 tccgccgaga ggagcggtgc cttcgtcttc tgccgcacga ccgagatgcc ggccggccgc
     2101 gtgctcggaa gccggagcgt ctccagctcg gcgggctcgg cgcgcatggc cggggcccgc
     2161 ttcttcttgt ccttgagccg gtccttgtgc cgcgagagcg ccttctcctg cttcgccagc
     2221 acctggtcta aggccacgag cgggtcctgg tcggtcccgc gggtcagcag gtcgacggag
     2281 ttcccatgca ggcggatctc ggcgatgtgc cggaagccct ccttggtgac gaccagctcc
     2341 gcccggatga ggctgggata gaagcgcgcg agcttctgga tgcgggcctc cgcggattcg
     2401 cgaaacgcgg gggtgggctt ccagtggcgc cctttgatcg tgatgtccat ggcttccttt
     2461 ctggggtgga tggcgcggtc aggcgtaccg gcgccgggcc cgggcgggca ggatgcggag
     2521 ggcttcgcga tacttcgcca ccgtgcggcg cgcgatcttg agccccgact cctggagcac
     2581 ctccgcgatg cgctgatcgc tcatcgggtc cagcttgtcc tcaccgttga tgagctggag
     2641 gatcttgtcc ttggccgcct tcgccgagac gtcgtcccct tcctcggtcg cgaggccgct
     2701 cgagaagaag tacttgagct cgaagacgcc ccgcggggtc tgcacgtact tgttcgtcgt
     2761 gacgcggctg accgtggatt cgtgcatgcc gatctggttc gcgatctgct ggagcgtgag
     2821 cggccggagc ccctcgacgc ccttctcgaa gaagtcccgc tgctcctcga cgatgcgccg
     2881 catcaccttg atcatcgtct tccgccgctg ctcgatcgtc tggatcagcc acttggccga
     2941 gttgagcttc tcggagatga acttccgctc ctcgtcgccg ccgctcttct tgcgcaggat
     3001 gtgctggtag gcgttgttga tccgaagccg cggcacgttc cggtcgttca gcgagacgac
     3061 gtagtcctcg ccgacgcgct cgaccacgag gtccggcgtc acgtagcgcg tctcctccgc
     3121 ggccacttcc tgccccggcc gcgggtcgag cgtcgcgatc aggtcccccg ccgcctgcac
     3181 ctcttccacg gtgcagcgca tggcgcgggc gatctcgctg aaacgcttgt tgaggaggtt
     3241 gtcgaactgc tcctggacga tccggccggc cagggtggcc tgaagattct tctgcgcgag
     3301 ctggatccag aggcactctt gcagattccg cgcgccgacg cccgcgggct cgaacgtctg
     3361 gatgatggcc agcacctcct ccaccttctc gggaggcgcg gccagcgcct cggccacctc
     3421 gtccaccgtc gtctggagat agccgttctc gtcgatggag ccgatcaggt agtcgccgat
     3481 cgagagctcc agctcgccca tgccggtcag gcggagctgc tcgctcaggt gctcgctcag
     3541 gctcttcacc gtgaccggca ccttctcgta gaactcctcg cccggatcgt ccgtgcgggg
     3601 cgccaccggg ccgtcccacg aggaatcggg ccagaagtcc tcccactcga ccttttggtc
     3661 ctcgggcgtc gcgtcgggag cctcgggctg ctccgactcc ttgagcccgg tctcctcctt
     3721 ggcgtcgtcg atctcctgga cctcttccac ctcgtccact tcctcgagca tcggattgcg
     3781 ctcgagctcc gccttcagcg cctgctgcag ctccagcgtc ggcatctgaa gcagcttcag
     3841 cgcctgctgc agacgctgcg tgatgatcag cgtctgccgc atctgctggt ggagaccggc
     3901 tttcatttcc atcgggggtt tcctctaatc cagacggaat cgctcgccca gatagatctg
     3961 gcgcgcgacc gggtcgtcgg ccagctcccg gctggtgccc tggagcagga tccgtccctc
     4021 gaacatgatg tacgcgcggt ccgtcgtgct caacgtctcc cgcacgttgt ggtcggtgat
     4081 cagaatgccc agtcctttcg cctgcagccg tccgacgatg tcctggatct cggccaccgc
     4141 gatggggtcg atccccacga acggctcgtc cagcagcatg aacttcggct cggtcaccag
     4201 cgcccgcgtg atctccacgc ggcggcgctc tccgcccgag aggttgtacc ccttccggtc
     4261 ggcgaggtgc gcgatgttca gctcctcgag gagctgcgcc agccgcgacg cccgctcctt
     4321 gcgcgagagc ggcagcgtct cgaggatcgc gagaatgttc tcgcgcaccg tcaggcgccg
     4381 gaagatcgac ggctcctgcg ccaggtaccc gatgcccagg cgcgcccgcc ggtgcatcgg
     4441 catccgggtg atgtcccgtc cgtccaccag gatctgcccc gcgtcggcgc ggagcagccc
     4501 cacgatcaga tagaacgtcg tcgtcttccc cgccccgttc gggccgagaa gaccgaccac
     4561 ctcaccctgg tccaccgtga tcgacacctt gtcgaccacc ttccactgcc catagaaccg
     4621 ctcgaggtcc cgcgcgacca gcccgctcac agggagtcga cgttcccctc ggtatccgtc
     4681 acctcggcgc ggacctcgcg ccggaggtgg aagtggtcca ggctcggatc gctctcgaaa
     4741 ccgtagccgg tcaccacgtc gtgctggcgc gtcaccttga cgaagccgtc cgagacgatc
     4801 ttgccggcgg tgttctgcca gcgcagggaa tcggtctcca tccggatgcc gctctcggtc
     4861 gtcaccacca cgttgccccg cgcctccaga tcgttcgtcc gctggtgcac ccgcccggtc
     4921 ttcgcgacca gggtcgagaa cttcttgcct tccttgtcga agaattcgat ccggaccgtc
     4981 ttcgcgtcga cgaggctctg atcgttgtac atcgcggcat aggacgccca gagggtccag
     5041 ttcttcttcc cctccgagct ctccgtgagg gtgaagtccc gcgcctcctg atccggcagc
     5101 cggacatcct ccccgctcgg ggtcgaagcc ggacgcgact ggcacccgaa cgccgtcagg
     5161 acggcggcca gcgcggccag gaccggcgcg cgcgccagga cccggctccg gcgaggtccc
     5221 gccggcggcc cggagcgcga ctctaccacg gcgcttcgag cgtggtcaaa ctgttgcgcc
     5281 gccatggtct tacccggcgc gcactccgag gcagtcatgg aggtgcagca ccccgacagg
     5341 ggttccgctc tcgtccccta cgacgaggga cgtgatgggc ccccccgggt tttcctccat
     5401 gacccggacg gcccgggcga tcgtcgcctc cggctcgatc gtcttcggcg tccggctcat
     5461 gacggcggac accggctggg cgaggtcccc gccgccgcga agcaggatcc gcttcaggtc
     5521 cccgtcggtg agcacgcccg cgagcttccc cgccccgtcc acgacggtgg tcatgccgag
     5581 ccgcttgttc aggatctcga gcagcgcctg gcgcagcgtc gcgccttcgt ccacgcgcgg
     5641 gagcgccgcg ccctggtgca tcaggtcgca cacccggtac gacgccgcgt gccccaggac
     5701 gccgccgggg tggagcacgg cgaagtcctc gcgcgtgaag cccttcagcc ggaggaggac
     5761 cacggcgagc gcgtcgccca tggcgagcat cgccgtcgtc gaagaggtcg ggacgaggtc
     5821 ctcgccgcac gcttcgcgcg gcgcgccgat ctccaatacg tggtcggagg cggcggccag
     5881 gctggattcg cggctggccg tcatcgccac cacgcagacg ccgcggcggc ggaaagcggg
     5941 aaccaggtcg atcagctcgc gggtctcgcc gctcttggag aggagcagcg ccgcgtcatc
     6001 ggtggcgacg atgccgagat ccccgtgcac cgcgtccacc ggatggacga acgtggcggg
     6061 cgagcccgtg ctggtcaggg tggcggcgat cttcttcgcg acgagccccg acttgccgac
     6121 gcccagggtc aggatctgcc cccgcgcttt ggacagtgcc tgaaccgcgg cttcgaactc
     6181 gtttccgatg cgcggcttga gctgaaggac ggcggccccc tcgaggtcca gcacctcgcg
     6241 cgcccaggcg acgaccttcg atgcggtgcc cgtttcggcc gccagggtca cgccctctcc
     6301 tcgcgctcgc gtcatgctcg ctgaccttcc agaaccaccg tatcggcaac cgcttgccgg
     6361 atcttcatca agctccgcag aagctcttcc agttcggcga ggggccactg cgactcgcgg
     6421 tcggagcggg cccgctccgg cgcgggatgg gtctcgatga agatcccgtc gacgcccgcc
     6481 gcgaccgcgg cccgggccag gaccgggatc atcgcgcgat cgccgcccgt ctcgacgccg
     6541 gccgggcgct gcaccgaatg ggtcgcgtcg aagaagaccg ggcagccgag agcgcgcatc
     6601 gtgggaaggc cgcggaaatc cacgacgaga tcgccgtggc cgaaggtcgt cccgcgctcc
     6661 gtcaggatga cgcccgccgc gccggcggcg cgcgccttgt cgacgacgtg gcgcatgccg
     6721 gaaggggaaa gaaactgtcc cttcttcacg tggagcggct ttcccgtctg cgccgccgcc
     6781 tcgatcagcg gcgtctggcg gcagaggaac gccgggatct ggagcaggtc gagcaccgcg
     6841 gccgcgtcgc cgacctcggt ggactcgtga acgtcgctcg tgaccggtac gtcggccgct
     6901 tcgcggatcc gcgccagcga gcgcagcccc tcggaggggc cggggccgcg gtaggaatcc
     6961 cccgacgtgc ggttcgcctt ggcgtacgag gccttgaata cgaacggcat gccgagcgat
     7021 ccggcgatcc gcttcacctc acgcgcgatc tccatcgctt cgccttcgtc ctcgagcgcg
     7081 caggggcccg cgatcagtcc gagctgctcg gggccgaagg cgaccgggcc gacccgcact
     7141 ggcgtgattc ggggagacgt agagctcatg gacgcggcgc gccggaggcg ctctcctggc
     7201 cggggcgcgc cggcgcgccc gcttcgcggc ggtggcgctc ctcgttggcg gcacggatga
     7261 atccgcggaa gagcgggtgc ggatcggacg gccgcgactt gagctccgga tggaactgaa
     7321 cgcccacgaa ccagggatgg tcgggcagct cgacgatctc gaccaggtcg cgcgcctcga
     7381 agaggcccgt cacgcgcatg ccgcgctcct ggaagacgtc gcgataggcg ttgttgaact
     7441 cgtagcgatg gcggtggcgc tcttccacgc gcgggcggcc atattcggcc tgggcgtggc
     7501 tgcccgccgc gagcgcggcc tcgtacttgc caagccgcat cgtcccgccc atctgggaaa
     7561 ccccctcctg ctcgggcatc aggtcgatca ccgcgtgcgg cgtcgaggga tcgaactccg
     7621 aggagtcggc gcccttcagt ccggccacgt cgcgcgcgaa ctcgatgacg gcgcactgca
     7681 tgccgaggca gatgccgaag aacggcacct tccgctcgcg agcgtagcgc gcggcctgca
     7741 ccatcccctc ggtgccccgg tcgccgaagc cgcccgggat caggatcccg tgcgtctggc
     7801 cgagcagcgc ctcggggccg tccagctcca cgcgctcgga gtcgacccag tcgatcttca
     7861 ccgtcacgcc gttcgagacc cccgcgtgct ggatcgcttc ctggatgctc ttgtaggcgt
     7921 cccggaggtg cgtgtacttc ccgacgacgg agaccgtcac cgactcgcgg gccgaccgca
     7981 tccgctcgga gaacgcgttc cagacgcgga ggtcggccgg acccgcgtcc aggcggagca
     8041 tctgcacgac caggtcgtcc aggcccccct tgtggaacat gaccggcacc tcgtagatcg
     8101 agggcacgtc gatcgcctcg atcaccgagc gggtcgggac gttgcagaag agcccgatct
     8161 tctccttcac ctcctgcggc agcggcacct cggtgcggca gaggaggatg tcgggctgga
     8221 tcccgatcgc gcggagctcc ttcaccgagt gctgcgtcgg cttggtcttg atctcgcgcg
     8281 cggcacccag gtgcggcacc agcgtcacgt ggatgaagag ggtgttcagc gaggagagct
     8341 ccagccttag ctggcggatc gcctccagga atgggagcga ttcgatgtcc ccgaccgtgc
     8401 cgccgatctc cacgatcgcc acgtccaccg gccgcccgcg cgtcagcgcc atcatgcggg
     8461 ccttgatctc gtcggtgacg tgcgggatca cctgcaccgt gcggcccagg tagtcgccgc
     8521 gacgctcctt ctggatgatc gcgtcgtaga tctgccccgc cgtgacgttg ttctcgcggc
     8581 ccatcgagac gccgaggaag cgctcgtagt gccccacgtc caggtcggtc tcggcgccgt
     8641 cgtcggtgac gtacacttcg ccgtgctggt aggggctcat cgtccccggg tcgacgttca
     8701 ggtagggatc gaacttctgc agcgtgaccg cgagcccgcg cgacttgagc agcgaaccga
     8761 tcgccgaagc ggcgatcccc ttgccgagcg acgagaccac gcctccggtc acgaacacga
     8821 acttggtcat cgccccactc cccccttggt catgatgctc ccccctcgct ccatcgcgtc
     8881 cgtgcccgcg ccagatcctc gggcgtatcg attccgatcg aatggtagtc gccgacggcg
     8941 acgtgtatcg ggatgccgtt ccagagcgcg cgcagctgct cgagccgctc gcaggtttcg
     9001 agcggtgcgg gcggcagcgc ggccagctcg cgcagggtcg tcagccggta ggcatagatc
     9061 ccgacgtgga gaagcggagt cgtcgcggcc gcgcccgtct gggccgcgcc cgtctgggct
     9121 cctcccggcg gagcgccgcc cgcaccatgc ggaatgagcg accgcgagaa gtagagcgcg
     9181 cgtcccatac ggtccagcac caccttcacc cggttggggt cggctggatc gtcccccgcc
     9241 gcgaagggcg cggccagcgt ggccatgcgc acctcgggcc ggtcgaagag cgagaccagg
     9301 tggtccagcc ccgccgggtt cagaaacggt tcgtccccct gcacgttcag gatgaccgga
     9361 tcggccgcca ggtcgggatc gcgcacgccg tccgcctggg accctcggcg ggtcatctcg
     9421 cagagcgcga tcgcggcctc ggccacccgg tcgagccccg cgggatggtc ggctcgcgtg
     9481 aggatcgtct cgcctcccgc gtccccgacg accttgacga tccgctcgct gtcggtcgcg
     9541 acccaaaccg cctccaaaga cgtcgcccgc cgggcgcgtt cgagcacccg cacgacgagc
     9601 ggcttaccgc cgagatcctg taagggtttt tcggggagac gggtggattg gagccgcgcc
     9661 ggaatgatcg cgacggcccc tgaagtcggg cgcgtgggca catcgcgctc cgctcggggg
     9721 gacgggagac ggtgcacgag tcatgctacc aagcggcgtg gaggtcgtca agcaaccgcc
     9781 gggccagcta cgtttgcggt gcggcctctg tggatcgccg tgggacccga tgccggtcga
     9841 accatgccgc ccatggcacg aggagccaca gcgtgagtcc gctccatgcg atcgtccgca
     9901 ccatctccgg ccccggcgtg gggggcgggc ccgccacgtc gcccagccag atcagggaaa
     9961 ggaacgcgag gaacagcacg aacccccacg tcccccgacg gtcgcgcgcc ctggtctgga
    10021 ccgcatagat cccgatcgcc gccacgtaca tcgcggtctc gatggtcacc tcgagcgcgg
    10081 ggtggttcca gaggccgagc ccgacgcggg tggcaatccc cgggacgagc tgcaggtcag
    10141 gtcggtgagt gatccagtcg aggaaccaat ggctgaagac ggcggcgccc aggaagagcg
    10201 caccggtccg gtatcgcttc cgcgcccagt agacgcctcc gatgagcacc gaccagagga
    10261 tcgccatcag gagactgtgg gagatcggat aggagtcgaa cgcgagcggg gtcgccttcg
    10321 tggagttcac gtcgatgtgg aatcgctcca ggcctaggag cacgaggggc ggccagatga
    10381 gatcgaggaa ctgcgccccg aagatgagcg tgccgagcga catcttcggc gcgacccgct
    10441 tccccgcgaa ggcgacagca aagtgtccga cgaacatcag gaagtccctc cggccggggc
    10501 tgcccccccg gcgggcccag tgggacggta gcgccgaggg cgttcaggat acgttgttcc
    10561 gcggccaaca ttttaccgcc gctggcgagt gtgaaaacga agacacgtgt cgctctctcg
    10621 ggaccttcga ggagcggtgg ccggactttc aagctcccgg gcgacataga ctcgccccgg
    10681 ggcgagttaa tttctatatt aactgcctcg gggcgaggaa gatcgttcta taaggcgctc
    10741 tgcgccgaag cgggcccggc cctcggcgct ccgcgcacga cgtataacga caactagtgc
    10801 gcgacgggct cgagcgagag gtccgcgatg cccttcttca cttcctccgc cgacttggcg
    10861 agcgccgact tctcttcggg cgtgagctcc agctccacga tcttttccag gccgcgcgag
    10921 cctaagatgg cgggca
//
LOCUS       DASSLZ010000331         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1519533, whole genome shotgun sequence.
ACCESSION   DASSLZ010000331 DASSLZ010000000
VERSION     DASSLZ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519533"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1235
                     /locus_tag="VFM00_12480"
     CDS             231..1235
                     /locus_tag="VFM00_12480"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7499107.1"
                     /translation="MIWDVLIVGAGPAGLSAALFTRMRRMSTLLLDTAVAGGQLASLY
                     PKKPVHDYPSYTYVMGDVLGRNFVDQANAMGAEIREGEHVTMIARDEEANLIRVTSTK
                     GSYEARSVIVATGGGAFEPRKINKPGEAELRGKGVGYGMPDIEESRGKRVLVIGGGDS
                     ALESALSLKDVSDVTLIHMLDKWQGMDSYVEEIAESKIRALLETETVEIRGNDRVESV
                     VVRNKKTEAVEEIPVDLVSINIGYLMDTKIIRQWGLTLTGNQIQVDNVMKTNIKGVFS
                     CGDIATYPGKYKLLITACSEGAAAGNSAYIYVKQPKKFTVGELYAAPKEEGDAQPKGS
                     "
     gene            complement(1232..2578)
                     /locus_tag="VFM00_12485"
     CDS             complement(1232..2578)
                     /locus_tag="VFM00_12485"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET7499108.1"
                     /translation="MPNFAPRELMERVRTNAERSGAAQADTFLEIVHFTEVRVRQREV
                     ELVQQSVIQGLGLRVFRDRRMGFMYTTDLRTPVLDEIVTRTIALAAEATPRDENKISE
                     EPAPAALELEIDDPGVLRLGPADLTALARAAEENAFAQDKRVQSTRDARCGVQSNEIH
                     FSSTFIPYQTYRASACWLSVEAVASQGTQKRTGGFADRKRIFADLETPERVGRKAATR
                     ALAKLGAAAVPSAKVPVIFEAEAAGIFLRGLFGAVSGSNVLEQRSFLAGKLGTAVASP
                     LVTIVDDGTMRRGLGSRPFDGEGVQSRRNVVVDRGILRRFLQTSVSARRMGVGTTGNA
                     GRGYDSIPEVQPTNFFIDRGGQRLERMIAETPRGLYVTDLAGFGIDVVSGEFSQQVEG
                     SWIEAGKLSKPVEGVTLGGRIPDMLMGIDAVGSDLEFRNNVASPSVRFKELTVGGV"
     gene            complement(2580..>2827)
                     /locus_tag="VFM00_12490"
     CDS             complement(2580..>2827)
                     /locus_tag="VFM00_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET7499109.1"
                     /translation="NFNFAVREGYLIENGKITRPVRSAVLIGNSLEFLQRIEGVGTDL
                     IVDQTRGTCGKDGQSVPVGVGQPTVRVSSITVGGTAI"
BASE COUNT          470 a          965 c          981 g          411 t
ORIGIN      
        1 actgcagatt gcagagcgtt cccccgacct ggaaccagac ggtgtcgaga ccgaggaacg
       61 gaacgcaggg cgcggcgcgc gcgcgctccg cgtggaacgc cggcgcggcg ccgcgcgtga
      121 gcaagggaag ggacgcgtcg aaagcgggag ccatggcgag ccgagcctag cacgctcttg
      181 tgctatcctc agcccgcgtt tccaccccct gttccctgga gccataccgc ttgatctggg
      241 acgtcctcat cgtgggtgcg ggccccgccg gcctctccgc cgccctcttc acgcgcatgc
      301 ggcgaatgtc cacgctcctc ctcgacacgg ccgtcgcggg gggccagctc gcctccctct
      361 accccaagaa gccggtccac gactacccct cgtacaccta cgtcatgggg gacgtcctcg
      421 gccggaactt cgtggaccag gcgaatgcca tgggcgccga gatccgtgag ggggagcacg
      481 tgacgatgat cgcgcgcgac gaggaggcga acctgatccg cgtcacgtcg acgaagggct
      541 cctacgaggc gcgcagcgtg atcgtggcga cggggggcgg cgccttcgag ccgcggaaga
      601 tcaacaagcc gggcgaggcg gagctgcgcg gcaagggggt gggctacggc atgcccgaca
      661 tcgaggagag ccgcggaaag cgcgtgctcg tgatcggcgg cggcgacagc gccctcgaat
      721 cggccctctc cctgaaggac gtctccgacg tcacgctgat ccacatgctc gacaagtggc
      781 aggggatgga ctcctacgtc gaggagatcg ccgaatcgaa gatccgcgca ctcttggaga
      841 cggagaccgt ggagatccgc ggcaacgacc gcgtcgaatc ggtggtggtc cggaacaaga
      901 agaccgaggc cgtcgaggaa atcccggtgg atctcgtctc gatcaacatc ggctatctca
      961 tggacaccaa gatcatccgc cagtgggggc tcacgctcac gggcaaccag atccaggtgg
     1021 acaacgtgat gaagacgaac atcaagggcg tgttcagctg cggggatatc gcgacctatc
     1081 cggggaagta caagctcctg atcacggcct gcagcgaggg ggcggcggcg ggcaattcgg
     1141 cctatatcta cgtgaagcag ccgaagaagt tcacggtggg cgagctgtac gccgcgccga
     1201 aagaggaagg ggacgcgcag cccaaggggt cctagacgcc cccgaccgtc agctccttga
     1261 agcgcacgct gggcgacgcc acgttgttcc ggaactccag gtcgctcccc acggcgtcga
     1321 tccccatcag catgtcgggg atccgcccgc cgagggtgac cccctcgacc ggcttcgaca
     1381 gcttccccgc ctcgatccag gatccctcga cctgctggct gaactccccc gacacgacgt
     1441 cgatgccgaa cccggcgagg tcggtgacgt agaggccgcg cggcgtctcg gcgatcatcc
     1501 gctccaggcg ctggccgccg cggtcgatga agaagttcgt cggctgcacc tcgggaatcg
     1561 aatcgtaccc gcgcccggcg ttccccgtgg ttccgacgcc catacgccgc gccgagaccg
     1621 aggtctggag gaagcggcgg aggatgccgc ggtctacgac cacgttgcgc cgcgactgga
     1681 ccccctcgcc gtcgaagggg cgcgagccca ggccgcgccg catcgtgccg tcgtccacga
     1741 tggtgacgag cggcgaggcc acggccgtcc ccagcttccc cgcgaggaag gagcgctgct
     1801 cgagcacgtt cgacccgctc accgcgccga acagcccgcg caggaagata cccgcggctt
     1861 cggcctcgaa gatcaccggg accttcgccg agggaacggc ggcggcgccc agcttggcca
     1921 gcgcgcgcgt cgcggccttc cgccccaccc gctccggcgt ctcgaggtcg gcgaagatcc
     1981 gcttccggtc cgcgaagccg cccgtgcgct tctgggtgcc ctgggaggcg acggcctcca
     2041 ccgagagcca gcaggcactg gcgcggtagg tctggtaggg gatgaaggtg ctcgagaaat
     2101 ggatctcgtt cgactggacg ccgcatcgcg cgtcgcgcgt ggactgcacg cgcttgtcct
     2161 gggcgaacgc gttctcctcg gccgcgcggg cgagggcggt gagatcggcc ggtccgagcc
     2221 ggagcacgcc gggatcgtcg atctccagct cgagcgcggc gggcgcgggc tcctccgaga
     2281 tcttgttctc gtcgcggggc gtcgcctcgg cggcgagggc gatcgtgcgc gtcacgatct
     2341 cgtccagcac gggggtgcgg agatcggtgg tgtacatgaa gcccatgcgg cggtcgcgga
     2401 agacgcggag gcccagtccc tggatcaccg actgctgcac cagctccacc tcgcgctgcc
     2461 gcacgcgcac ttcggtgaag tgcacgatct cgaggaaggt gtcggcctgg gcggcgccgg
     2521 aacgctcggc gttggtgcgg acccgctcca tcagctcgcg cggcgcgaag ttcggcatgc
     2581 tagatcgccg tcccgccgac cgtaatcgag gagacgcgca cggtgggctg tcccacgccc
     2641 accgggaccg actgcccgtc cttgccgcag gtgccgcgcg tctggtccac gatcaggtcg
     2701 gtgcccacgc cctcgatccg ctggaggaac tcgagggagt tgccgatcag cacggcgctc
     2761 cggaccgggc gcgtgatctt cccgttctcg atcaggtacc cctcgcggac ggcgaagttg
     2821 aagttgc
//
LOCUS       DASSLZ010000332         6667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1520126, whole genome shotgun sequence.
ACCESSION   DASSLZ010000332 DASSLZ010000000
VERSION     DASSLZ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520126"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFM00_12495"
     CDS             complement(<1..201)
                     /locus_tag="VFM00_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499110.1"
                     /translation="MKPWVLRAVALAAVGAAAGIALVRVAPIAEPSSSAAVRADPNLL
                     ADSICDCDARAINPDCRTRIIPA"
     gene            251..802
                     /locus_tag="VFM00_12500"
     CDS             251..802
                     /locus_tag="VFM00_12500"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7499111.1"
                     /translation="MEPIQVAGAFWSVFALYWLVSSFGRNPARRRESPLARLAHVLYM
                     GAAFILLYSRDHRFGLLDRRFVPMARWIEWAGALMTAAGVALAIWARRHIGKHWSAEV
                     TIRQDHELIRTGPYARIRHPIYTGILLALFGTALILGEWRGLVAVGLAVMGFWWKARR
                     EESLLRQAAPVSNFPLFASAFGC"
     gene            902..2323
                     /locus_tag="VFM00_12505"
     CDS             902..2323
                     /locus_tag="VFM00_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET7499112.1"
                     /translation="MVRKLLLLAIVSGALVSSSPGFTLTIPSNLWVQQPPPAQDLPPD
                     RLGVYAGRGWNHMRYASGLGKMVLFDGYAELPVYPFDSIFANALWLYDPVENRLSLEK
                     VDNWTTQNGFCEPLPENELDPTPWDRHFYSCFVYSPAKNAVYLWAGANRTIPDDMIGE
                     TWTYDFATHAWREITGPHPYTVYEQACAYDSDLDRMILFAGTDHEYGTGDRTYALDLA
                     TETWTDRAPVSSPMPRMGQNMVYDPARRVTWMFAGAPWGSADSELWCYDDTANTWSQV
                     PKQDPWPSARRFAHFARDSRHDVILMWGGITAEDVTLSDTWVFRPASQTWEQLFPEVF
                     PANPARFYSVDLDYDEGNDFFVLNLGGTFWLFRYSGSVADADSEGDASAAPSLRITSS
                     NPAPRGATLAISLPRSEQVEVGIFNASGHRIETLASGIWERGPHTLQWSGRAGGVPAA
                     SGVYFARLAAAGRVVTKKFTLLR"
     gene            complement(2357..3907)
                     /locus_tag="VFM00_12510"
     CDS             complement(2357..3907)
                     /locus_tag="VFM00_12510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7499113.1"
                     /translation="MTRSLNPDRPTLQKLAAGTIAALLAAKLLLHLAALTQYGYFRDE
                     LYYLASTDHLAWGYVEHPPLSIALLALVRAVLGDSLPALRFVPLVAGTAVVLLTAILA
                     RRLGGGGFAQGIAALAAVMAPAFLGTGRYYSMNALDLLFWTLASLATLRALERGRPRD
                     WAALGVLLGLGLLNKISMGWFGGGLAIGLLATRQRRALLGAGPWLAAAIALAIFAPHV
                     IWQIRNGWPTLEFIRNATSLKMAATTPGDFVKDQILTMNPGAAPLWIAGLLYGLFALR
                     GRPGQVFSWIYLSVLALLLAQGHSRASYLVVAYPPLLALGGVAVERSTGTTWRWVRPA
                     LAALIIMTGLVAVPLALPVLPARTFVRYQSALGMAPRTEERNAMGALPQDYADMFGWE
                     EMAGLVAKAYDQLTPEERGRCLIFGQNYGEAGAIDVIGRKRGLPPAISGHNSYWLWGP
                     RRREWDVVIIIGGDRQDNAAFFDRIEIVGQTRSEWAMPYERGLDVSIARGPRMPLAEA
                     WPRLKQYI"
     gene            3983..5593
                     /locus_tag="VFM00_12515"
     CDS             3983..5593
                     /locus_tag="VFM00_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7499114.1"
                     /translation="MARSASPIGAAAIRNRLWRRAAAPLSVLLLVLGCAHAGRLTFVE
                     QPLTPADTTGGHHPERALRHPVRERALFLPDTVIGFQPGTGDSSRRYLGKLLDGYTAD
                     TLNILLFGDNRPGYRCTKLAPELAVIQKGLSPNPLRLGRALITIPWMLVKGLLPPDVA
                     IVRDIPPFVRGLPTYGREKQVLHAIMAKLDTLKMQNKKVAAAINTGDLVYDGTNPAQW
                     RRFLRLSEPLMTRVPYFAMPGNHEKTHTETGVANWRVATGLPIAGDRFYYCFDSADGW
                     VRFVALDTNPITMPGVHWSKEVQVKYSKEQVDWLTARLKEHHGPSFVFFHSPPMSAGY
                     HRHDWNMDPIMQERREQIVRAMRDAGISVLASGHEHDYERALMTFPDGGVMICIVQGG
                     GGAPLHPLPPPAKAAQLFAEYHVAGGTIKPENVYTAAVNNFTLIQLWFGGGQLQTYAV
                     DKKGNVKLADLVKIDLTRYGTPKIDQHKVPIAATAPVHPSTSEVKREAGIQAKVDTTA
                     ASRRIESHPPPGKKKAAPVRRTTTRRRR"
     gene            complement(5604..>6667)
                     /locus_tag="VFM00_12520"
     CDS             complement(5604..>6667)
                     /locus_tag="VFM00_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499115.1"
                     /translation="NWVEGGTTGPPLGKGALAKYGDHPTWLSVDRADREAMDVYTRRE
                     RGVPKRTHGDWDSNADALNKDWEGRPHHSDAYICAWFASRQWVRLFKDFVADPAVWGR
                     MQAFSRSTFDPSRDWDYARKISFYGGHWNGNGGPTSLGSAFSARTAATSPDLLANAVL
                     NYMYGRCLTTKPGALRTEAEKLLLSWGAMPYKGPVNPTLPSPAPETMQFVRLQVHRID
                     NINGDDGFGGGEMDWYSRAAIDGQRYWSGLIDEHNNFDFDKSPYAPWTMTKAVTSSTP
                     EVPIIFQLMELDYSDDDQVDVNPRSGAQSLVLRYSLTGGTLRGDVTGPAAFTVEGKGD
                     CDCARLKMSVDRLSGSCLK"
BASE COUNT         1083 a         2285 c         2244 g         1055 t
ORIGIN      
        1 cgccgggatg atccgggtgc ggcagtcggg gttgatcgcc cgggcatcgc agtcgcagat
       61 cgaatcggcg aggaggttgg gatcggcgcg gaccgcggcg gaggacgacg gctcggcgat
      121 gggggcgaca cggacgagag cgatcccggc cgcggctccg accgcggcga gagcgacggc
      181 gcgcaggacc cagggtttca tcgaagtcag ggtacgcgaa atcgaagacc tcaggtaccc
      241 tgcgcggtcc atggaaccga tccaggtcgc cggagcgttc tggagcgtct tcgcgctcta
      301 ctggctggtc tcgtcgttcg gacggaatcc ggcccggcgc cgggagagcc cgctcgctcg
      361 actcgcgcac gtgctctaca tgggcgcggc gttcatcctg ctctattcgc gcgaccatcg
      421 gttcggattg ctcgaccgcc gcttcgttcc gatggcccgg tggatcgagt gggcaggggc
      481 gttgatgacc gcggcgggcg tggcgctggc gatctgggcc cggcgccaca tcggcaagca
      541 ctggagcgcg gaggtgacga ttcgtcagga ccacgagctg atccgaaccg gcccctacgc
      601 gcgcatccgc cacccgatct acaccggcat cctcctcgcc ctcttcggaa cggcgttgat
      661 cctgggcgag tggcgggggc tggttgccgt agggctggcc gtgatgggat tctggtggaa
      721 ggcgcggcgc gaggaatcgc tgctcaggca ggcggcgccg gtctccaact tccccttgtt
      781 cgcttccgcg ttcgggtgct aactctacgg caacccccac caggcgcgcc ggactcgccg
      841 ggacctcccc cccgagcgct accctccgcg tcgcaccgcg ctccaccctc ggggaggcgc
      901 catggttcgg aagctccttc ttctcgcgat cgtttcggga gccctggtct cctcttcgcc
      961 gggctttacg ctgacgattc cttccaatct ctgggttcag cagccgccgc ccgcgcagga
     1021 tcttcccccc gatcggctcg gggtctacgc cggccgggga tggaatcaca tgcgctacgc
     1081 ctcgggcctc gggaagatgg tcctgttcga cggatacgcg gagctccccg tgtacccgtt
     1141 cgacagcatc ttcgcgaacg cgctctggct ctatgatccc gtcgagaacc gtctctcgct
     1201 ggagaaggtc gacaactgga cgacccagaa cggattctgc gagccgctcc ccgagaacga
     1261 gttggacccg accccctggg accggcattt ctattcctgc ttcgtctatt ccccggcgaa
     1321 gaacgcggtc tacctgtggg cgggggcgaa ccgaacgatc cccgacgaca tgatcggcga
     1381 gacctggacc tacgacttcg cgacgcatgc ctggcgcgag atcaccggcc cgcaccccta
     1441 cacggtctac gagcaggcct gcgcgtacga ttccgacctc gaccggatga tcctcttcgc
     1501 gggcaccgat cacgagtacg gaaccggcga caggacctat gccctcgacc tggccacgga
     1561 gacgtggacc gaccgggcgc cggtttcgag cccaatgccg cgcatggggc agaacatggt
     1621 ctacgacccg gcgcggcgcg tgacctggat gttcgccggc gcgccctggg gcagcgccga
     1681 cagcgagctc tggtgctacg acgacacggc caacacgtgg agccaggttc cgaagcagga
     1741 tccgtggcct tccgcgcgcc gcttcgcgca cttcgcccgc gacagccggc acgacgtgat
     1801 cctcatgtgg ggcggcatca cggccgaaga cgtcacgctg tccgacacct gggtcttccg
     1861 cccggcctcg caaacctggg agcagctctt cccggaggtg ttcccggcga atcctgcccg
     1921 cttctactcc gtcgatctcg actacgacga gggaaacgac ttcttcgtgc tgaacctggg
     1981 cggaacgttc tggctgttcc gctactccgg ctcggtcgcg gacgccgatt ccgaggggga
     2041 cgcctccgcc gcaccctcgc tgcggatcac gtcctccaac cccgcgccgc ggggcgcgac
     2101 gctcgccatc tcgcttccga gatcggagca ggtcgaagtg gggatcttca acgcctcggg
     2161 gcaccggatc gagaccctcg ccagcgggat ctgggagcgc gggccgcaca cgctgcagtg
     2221 gtccggacgt gccggcggcg tgccggcggc ttcgggagtc tatttcgcgc ggctcgccgc
     2281 ggcggggcgc gtggtgacga agaagttcac gctgctccgg tagcgcccgg cgtccgcgcg
     2341 ccgtcgaacg cggggatcag atgtactgct tcaggcgcgg ccacgcttcc gccagaggca
     2401 tcctcggccc acgcgcgatc gagacgtcca gtccccgttc gtagggcatc gcccactccg
     2461 accgcgtctg ccccacgatc tcgatccgat cgaagaacgc ggcgttgtcc tgccggtcgc
     2521 cgccgatgat gattacgacg tcccactcgc gccgccgggg cccccagagc caatagctgt
     2581 tgtgtccgct gatcgcgggc gggaggcccc gcttccgccc gatcacgtcg atcgctccgg
     2641 cctcgccgta gttctggccg aagatgagac agcgcccgcg ctcctcgggc gtcagctggt
     2701 cgtaggcctt cgcgaccagg ccggccatct cctcccagcc gaacatgtcc gcgtagtcct
     2761 gcggcagcgc tcccatggcg ttccgttcct cggtccgggg cgccatgccg agcgcggact
     2821 ggtagcgcac gaacgttcgg gccggcagca ccggaagcgc gagcggcacg gcgaccagcc
     2881 ccgtcatgat gatgagggcc gcgagcgcgg gtcggaccca gcgccaggtg gtcccggtcg
     2941 aacgctcgac cgcgacgccg cccagcgcga gcagcggcgg ataggcgacc acgagatacg
     3001 atgcgcggct gtgcccctgc gccaacagca gcgccagaac cgagaggtag atccaggaga
     3061 agacctgccc cggccgtccc cgaagcgcga agaggccgta caggagcccc gcgatccaga
     3121 gcggtgcggc gccgggattc atggtcagga tctgatcctt cacgaaatcg ccaggcgtgg
     3181 tcgcggccat cttgagcgag gtcgcgttcc ggatgaactc cagggtgggc cagccattcc
     3241 ggatctgcca gatcacgtgc ggggcgaaga tggcgagcgc gatggcggcg gcgagccaag
     3301 gcccggcccc gagcagcgcc cggcgctgcc gcgtggcgag aagtccgatc gcgagacctc
     3361 cgccgaacca tcccatgctg atcttgttca gaaggcccag cccgagcagc acgcccagcg
     3421 cggcccagtc gcgcggccgc cctcgctcca gtgcccggag cgtcgcgagc gaggcgaggg
     3481 tccagaagag gagatcgagg gcattcatgg aatagtagcg gccggtgccg aggaacgccg
     3541 gcgccatcac ggccgcgagt gcggcgattc cctgtgcgaa gcccccgccg cccaaacgcc
     3601 tcgccaggat cgccgtcagg agcacgacgg cggtgccggc cacgaggggg acgaagcgca
     3661 gcgcgggaag ggagtctccc aggaccgcgc gcacgagcgc caggagtgcg atcgagaggg
     3721 gcgggtgctc gacatagccc cacgcgagat ggtccgtgct cgcaagatag taaagctcgt
     3781 cccggaagta tccgtattgc gtgagcgccg ccagatggag caggagcttg gccgcgagaa
     3841 gcgcggcgat ggtgccggcc gcaagcttct gaagggttgg cctgtccggg tttagggagc
     3901 gggtcacggc cggatactga catacggtgg atcgtttcct gctacgattc tggcgggggc
     3961 tgaggggaag gagatttcca tcatggctcg aagcgcgagc ccgatcggcg ccgctgccat
     4021 caggaatcgc ctgtggcggc gtgccgcggc accgctttcc gttctgcttc tggtcctcgg
     4081 ctgcgctcat gcggggcggc tgacgttcgt cgaacagccg ctgactccgg cggacacgac
     4141 gggcggccac caccccgagc gcgcgctgcg ccatccggtg cgcgaacgcg cccttttcct
     4201 acccgacacc gtgatcggct ttcagcccgg cacgggagac tcgtcgcggc ggtatctggg
     4261 caagctcctc gacggctaca cggcggacac gctgaacatc ctgctgttcg gcgacaaccg
     4321 gcccggctac cgctgcacga agctcgctcc cgagctggcc gtcatccaga agggtctctc
     4381 gccgaacccg ctccgattgg gacgcgcgct gatcaccatc ccctggatgc tggtgaaggg
     4441 cctccttcct cccgacgtgg cgatcgtgcg ggacattccg ccattcgtgc gtggcctgcc
     4501 gacctatggg cgggagaagc aggttctgca cgcgatcatg gcgaagctgg acacgctgaa
     4561 gatgcagaac aagaaagtgg cggccgcgat caacaccggg gacctcgtct acgacggcac
     4621 gaacccggcc cagtggcgcc gattccttcg cctcagcgaa ccgctcatga cgcgggttcc
     4681 ctatttcgcg atgccgggga accacgagaa gacccacacc gagaccggcg tggcgaactg
     4741 gcgggtcgcg accggactcc cgatcgcggg cgaccgcttc tactactgct tcgactccgc
     4801 ggacggctgg gtccggttcg tggcgctcga caccaacccg atcacgatgc cgggggtcca
     4861 ctggtccaaa gaggtgcagg tgaagtactc gaaggaacag gtcgactggc tgacggcccg
     4921 gctcaaggag caccacggcc cgtcgttcgt gttctttcac agtccgccca tgtcggccgg
     4981 ctatcaccgt cacgactgga acatggaccc gatcatgcag gagcgccgcg agcagatcgt
     5041 ccgggccatg cgcgatgcgg gtatctccgt cctggccagc ggacacgagc acgattacga
     5101 acgcgccctc atgaccttcc ccgacggcgg cgtgatgatc tgcatcgtcc agggcggagg
     5161 cggcgcgccg ctccaccccc ttcctccgcc ggcgaaggcg gcccagctct ttgccgagta
     5221 tcacgtggcc ggagggacga tcaagccgga gaacgtctac accgcggcgg tgaacaactt
     5281 caccctgatc cagctctggt tcggcggcgg ccagctgcag acctacgcgg tggacaagaa
     5341 gggaaacgtc aagctggccg atctcgtgaa gatcgatctc acgcgctacg ggacgccgaa
     5401 gatcgaccag cacaaggtcc cgatcgcggc cacggcgccg gtccatccct cgacgtcgga
     5461 agtgaagcgc gaggcgggca tccaggcgaa agtcgatacg acggcggcga gccggcggat
     5521 cgagagtcac ccaccgccgg gtaagaagaa ggcggcgccg gtgcggcgaa cgaccacgcg
     5581 gcggcgccgc taggcggcgc cgttcatttg agacagcttc cggagaggcg atcgacgctc
     5641 atcttgaggc gggcgcagtc gcagtctccc tttccctcga ccgtgaaggc cgcggggccc
     5701 gtgacgtccc cgcgcagcgt gccgccggtc agcgagtagc gcaggacgag gctctgcgcg
     5761 cccgagcgcg gattcacgtc cacctggtcg tcgtcgctgt aatcgagctc catcagctgg
     5821 aagatgatcg gcacttcggg cgtcgaggac gtgaccgcct tggtcatcgt ccagggcgca
     5881 tagggagact tgtcgaagtc gaagttgttg tgctcgtcga tcagccccga ccagtagcgc
     5941 tgcccgtcga tcgccgcgcg gctgtaccaa tccatctccc cgcccccgaa gccatcgtcc
     6001 ccattgatgt tgtcgatgcg gtggacctga agccgcacga actgcatggt ctcgggcgcg
     6061 gggctcggca gcgtcggatt gaccgggccc ttgtagggca tggcgcccca ggagagaaga
     6121 agcttctccg cttccgtccg gagcgcgccc ggcttggtcg tcaggcagcg cccgtacatg
     6181 tagttgagca ccgcgttcgc cagcagatcg ggcgacgtcg cggccgtccg cgcgctgaaa
     6241 gcgcttccca ggctggtggg tccgccgttg ccgttccagt gaccgccgta gaacgagatc
     6301 ttccgggcgt agtcccagtc gcggctgggg tcgaacgtac tccgcgagaa cgcctgcatc
     6361 cggccccaga ccgccggatc ggccacgaag tccttgaaga gacggaccca ctgccggctc
     6421 gcgaaccagg cgcagatgta ggcgtccgaa tgatgcggac gtccctccca gtccttgttc
     6481 agggcgtcgg cgttgctgtc ccaatcgccg tgcgtccgct tgggaacgcc ccgctcgcgc
     6541 ctggtgtaca cgtccatcgc ttcacggtcg gcgcgatcca ccgagagcca ggtggggtgg
     6601 tcgccatact tggccagggc gcccttgccg agaggcgggc cggtcgtccc tccttccacc
     6661 cagttgc
//
LOCUS       DASSLZ010000333         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1742082, whole genome shotgun sequence.
ACCESSION   DASSLZ010000333 DASSLZ010000000
VERSION     DASSLZ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742082"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1417
                     /locus_tag="VFM00_12525"
     CDS             173..1417
                     /locus_tag="VFM00_12525"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET7499116.1"
                     /translation="MNHIGRLDWGPLAWTWGAAHLIYLGAAALALRGRGPWSRRPALA
                     LAVILGVGLVPRLLLLPTAPSLSEDLYRYLWDGRLVANGWNPYPRPPSDPTFAPWHDD
                     VYRHLNHAGVPTVYPPLAQWLFALAWKLGGTPLAWKAVLLALEAALVAGLMALLRRRG
                     HPSERLLLYYWNPLVVVECCGSGHVDLAAAAFLIAALAFLEAGRRARAGGAFGAAVLV
                     KLVPLLMVPALARRRAWKTLATAFLAIALLYLPFRGAGARLWEGLRTYARHWEYNGPV
                     YAAIRPLFSNGDDPRLVLAAGLVLALLVIAWRARTLSGALLASWMAFLILSPTVYPWY
                     LIPAIALLPLHPDPGLLLFSGTVALTYLPLPLYRATGVWLVPGWVLGVEYGSLAAVWG
                     VAAWRRLPQPRRAAWRTETPPT"
     gene            complement(1372..2853)
                     /locus_tag="VFM00_12530"
     CDS             complement(1372..2853)
                     /locus_tag="VFM00_12530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7499117.1"
                     /translation="MNSFLAALSAPVRESLLALHLVVWSLLAAYGIHRLHLLRLFRRR
                     RAPMPRPLPPPEWPLVTIQLPVYNERYVAERLLDAAAAMDYPRDRLEIQVLDDSTDAT
                     SGLVAARVAELRARGVDAVHLRRAARDGFKAGALQNGLRHAKGELLAIFDADFVPPPS
                     FLRELVPYLNEPDVGMAQARWRHLNQDYSMLAQAQAISLDGHFVVEHSARMDARRFFN
                     FNGTAGILRRRCIEDAGGWQWDTLTEDLDLSYRAQLRGWRFVFAPDVGCPGELPVEMN
                     AFKAQQYRWVRGSIQVARKVLPDVWRAPLPFAVKVEATFHLTINVAYVLLLLVAAIAY
                     PVVIARIEEKSLLFTVVDAALFLTASIPALIYFAAAQRETRGDWKRQLRFVPLVLSLG
                     LGLAVNNTRAVLSGLRNASAEFHRTPKFRIERRSDGWRGKRYRSPLSLWAAAEIGLGL
                     YFGWAMLALARAGMLAPLPFLVLYLVGFLYVGGVSVLHAARRG"
     gene            2986..>3225
                     /locus_tag="VFM00_12535"
     CDS             2986..>3225
                     /locus_tag="VFM00_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499118.1"
                     /translation="MARGFVVGNGTVLATGDRHGALHELYAPHFAPEHQLLRRPARIG
                     LHVDGLFHWLEDSFEAAAVRGEAAPIADLSLRGQGL"
BASE COUNT          431 a         1165 c         1145 g          484 t
ORIGIN      
        1 cagaggccgc gcattcccgg atcggacgag gccagctcct ggggcgtgcg ttgaccctcc
       61 gccgcgcccc gcgtacgatc ccgcccggga tccgcgccgg catccacgcc ggcggccggc
      121 ttcagcccct tgcggcgcgc actcatcgga ccggggtcac cctccgcact tcgtgaacca
      181 cattggacgg ctggactggg gaccgctcgc ctggacctgg ggcgcggcgc acctcatcta
      241 cctcggcgcg gccgcgctcg cgcttcgcgg gcgagggccg tggtcgcgcc ggccggcgct
      301 cgcgctcgcg gtcatcctgg gggtgggcct ggtcccgcgc ctcctcctgc ttcccaccgc
      361 cccatcgctc tccgaggatc tgtaccgcta cctctgggac ggccgcctgg tggcgaacgg
      421 ctggaacccc tatccgcggc ctccctcgga tcccacgttc gcgccgtggc acgacgacgt
      481 ctaccggcac ctgaaccatg ccggcgtgcc caccgtctac cctcccctgg cccagtggct
      541 cttcgccctc gcctggaagc tgggcggaac gccgctcgcc tggaaagcgg tgctcctcgc
      601 gctggaagca gcgctcgtgg cgggtctcat ggccctgctg cgccggagag gacacccgtc
      661 ggagcgtctc ctactctact actggaatcc gctggtcgtg gtcgaatgct gcggctcggg
      721 gcacgtggac ctggccgcgg cggcgttcct gatcgcggcc ctggcgttcc tggaagcggg
      781 gcgccgcgcg agggcgggcg gcgcgttcgg cgcggcggtg ctcgtgaagc tggttccgct
      841 gctgatggtc ccggcgctcg cccggcgtcg cgcgtggaag acgctcgcga cggcattcct
      901 ggcgatcgcg ctcctctacc tgccgtttcg cggggcgggg gcgcggctct gggagggcct
      961 gcgcacctat gcccgccact gggagtacaa cggtcccgtc tacgcggcga tccggccgct
     1021 cttctcgaac ggggacgatc cgcgcctcgt tctcgccgca ggactcgtgc tcgcccttct
     1081 cgtcatcgcc tggcgcgcac ggacgctgag cggcgctctg ctcgcctcct ggatggcgtt
     1141 cctgatcctc tcgccgaccg tctacccgtg gtacctgatc cctgcgatcg cgctcctgcc
     1201 gctccatccc gacccgggac tcctcctctt ctcgggcacg gtggcgctca cctacctccc
     1261 gctgccgctc tatcgggcga ccggcgtgtg gctggtcccg gggtgggttc taggcgtcga
     1321 gtacggatcg ctggcggccg tgtggggcgt ggcggcatgg cgccgcctcc ctcagccgcg
     1381 gcgcgcggcg tggaggacgg agacgccccc cacgtagagg aagccgacca ggtagaggac
     1441 gaggaagggg agcggcgcga gcatccccgc gcgcgccagc gccagcatgg cccagccgaa
     1501 gtagagtccc agcccgatct cggccgccgc ccacagcgag agtggcgagc gatagcgttt
     1561 gccgcgccac ccgtcgctcc gccgctcgat ccggaacttc ggagtgcggt ggaactcggc
     1621 cgaggcgttc cgaagcccgc tcagcaccgc ccgggtattg ttcacggcca gccccagccc
     1681 cagggagagg accaagggga cgaagcgcag ctgccgcttc cagtcgccgc gcgtctcccg
     1741 ctgcgccgcg gcgaagtaga tgagcgccgg gatcgaggcc gtgaggaaga gggccgcgtc
     1801 cacgaccgtg aagaggaggc tcttctcctc gatccgcgcg atcaccacgg gataggcgat
     1861 cgccgcgacc aggagcagca gcacgtacgc cacgttgatg gtgaggtgga acgtggcctc
     1921 caccttgacc gcgaacggaa gcggggcgcg ccagacgtcc ggcagcacct tccgggcgac
     1981 ctggatcgag ccgcgcaccc accggtactg ctgcgccttg aacgcgttca tctcgacggg
     2041 cagctctccg gggcacccca cgtccggcgc gaagacgaag cgccatccgc ggagctgggc
     2101 ccggtaggag aggtcgagat cctcggtgag cgtgtcccac tgccatcccc cggcgtcctc
     2161 gatgcagcgc cggcgcagga tccccgccgt cccgttgaaa ttgaagaagc gccgcgcgtc
     2221 catgcgcgcc gaatgctcga ccacgaagtg gccgtccagc gagatcgcct gcgcctgggc
     2281 cagcatcgag tagtcctggt tcaggtggcg ccagcgcgcc tgcgccatgc cgacgtccgg
     2341 ctcgttcagg tagggcacca gctcgcgcag gaacgagggg ggtggcacga agtcggcgtc
     2401 gaagatcgcg agcagctccc ccttggcgtg gcggaggccg ttctggagcg cgcccgcctt
     2461 gaatccgtcc cgagccgcgc ggcggagatg caccgcgtcc acgccgcgcg cgcggagctc
     2521 ggcgacccgc gcggccacga gcccgctggt ggcgtcggtc gagtcgtcca gcacctggat
     2581 ctccaggcgg tcgcgcggat agtccatggc cgcggcggcg tccagcaggc gctcggccac
     2641 gtagcgctcg ttgtagacgg gaagctggat cgtgacgagg ggccactcgg gcggcggcag
     2701 ggggcgcggc attggcgccc ggcgcctccg gaagagacgc aggagatgaa gacggtggat
     2761 cccgtaggcc gcgagcaggc tccagacgac caggtggagc gcgagcagcg actcccggac
     2821 cggcgcggac agcgcggcga ggaacgaatt catcgaggcg gtcatcgtac cgcgcacgca
     2881 tcggtccatg ttgcgtgttc ttccgccggt ccgtgttgat tgttccgggc cgcgcgggta
     2941 atgtgcggga cggggtgggg tgacgcggga aacgggagga tcactgtggc gcgcggcttc
     3001 gtggtgggca acggaacggt cctcgcgacc ggcgacaggc acggcgcgct ccacgagctc
     3061 tacgcacccc atttcgcccc cgagcatcag ctgctccgcc gtccggcccg catcgggctg
     3121 cacgtggacg gcctcttcca ctggctggaa gactccttcg aggccgccgc cgtgcgcggt
     3181 gaggccgcgc cgatcgccga cctctccctc cgcgggcagg ggctt
//
LOCUS       DASSLZ010000334         8160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1864498, whole genome shotgun sequence.
ACCESSION   DASSLZ010000334 DASSLZ010000000
VERSION     DASSLZ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864498"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFM00_12540"
     CDS             complement(<1..415)
                     /locus_tag="VFM00_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499119.1"
                     /translation="MARAVSRNVQLLAHVSNYTRYSACTAYVAPDGREYAVLGTETGT
                     SILNVTNPSAPYEVGFIPGLTSWWREMKQYRTWLYVSTEAIGGGIQIIRMTDPEHPVL
                     VNTYTTAFNREHTVTVDTTRALLILNGTRATNTMTG"
     gene            complement(617..1603)
                     /locus_tag="VFM00_12545"
     CDS             complement(617..1603)
                     /locus_tag="VFM00_12545"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7499120.1"
                     /translation="MTGAAPLAVVTGASGFVGSHIVDELLLRGARVRAVVRGSSSRRW
                     LEQKPVELADAALDDPGALARAVEGADWIVHAAGLIRARNAAEFHEANVVGTERLLRA
                     ADGGTLRRFLFISSQAAAGPSLTGLPVTEDQRPHPVSAYGESKLRAEQLVVMAADRLP
                     VSVIRPPAVYGPRDTAILKAFVAGKWHLEPVLRKRGRFSIVHVSDLVTAVHAALTQEA
                     AVGQVFFACEPDVTDYDELGRSVREALRTWTVRVAIPQWTLAGIAAVAESWARAAGKA
                     PLLSREKLAEIAAGDWICSSRKIRERLGWTPRVGLYEGIRATAEWYREVGWL"
     gene            complement(1600..2709)
                     /locus_tag="VFM00_12550"
     CDS             complement(1600..2709)
                     /locus_tag="VFM00_12550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7499121.1"
                     /translation="MEVVPVQTAKDLDRFIRYVYDRYRNEPHWVPPLPSDEKARLTPG
                     KNPYFEHAEAAYFLALDGGRVVGRIAATVDRNHDAFQGERQAAFGFFEADSPAATAAL
                     LVAAERWGREKGAQVLRGPLSFTTNDECGLLIEGFDHRPMLLMPYNVREYAVWIEAAG
                     LAKAKDLYEWRVPVPETPPPVFQRIASMARKRDRITVRPLEMKKFNEELGRVKTVYNA
                     AWERNWGFVPMTDAEIDHMASQLKPAVVPDLVQFAEVDGAPVGFGLILPDVNVALAKV
                     KGKLFPFGIVKLLWTLPRIKEGRLLALGVLGEYQRRGVGALLVEALMRATRRRGYAMT
                     EVGWTLEDNDSVHQLILGSGGTRSAIYRIYEKRLA"
     gene            complement(2727..3893)
                     /locus_tag="VFM00_12555"
     CDS             complement(2727..3893)
                     /locus_tag="VFM00_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HET7499122.1"
                     /translation="MAQRAGFYPYFLPISENHGTEVTIHGKRMIMAGSNNYLGLTKHP
                     KVMAAAEDALMRFGTSNNGSRFLNGTLELHAILEERLAKFMKKEAALVYSTGYLTNLG
                     SISALAEKGDVVILDKDAHACIVDGARLSHGQVLRFRHNDLRDLERVLEKVPAKAGKL
                     VVVDGVYSMEGDIAPLPDIVRLVREYNARLLVDDAHGIGVLGARGAGACEAQNVEDQV
                     DLVMGTFSKSFASLGGFIAGPKEVMDYLRHHARAMIFSASMTPSSVAAALAALDVIES
                     DEGPVLRERLRVIADKMRTGFKAQGLEVGSGKGTPIIPIFIGDRMKCMHWWRKIYDRG
                     VFVNAILVPAVAPGRDLLRTSYMATHQDSQLDTILRVVSEVRQQLGAELASQAE"
     gene            4200..5198
                     /locus_tag="VFM00_12560"
     CDS             4200..5198
                     /locus_tag="VFM00_12560"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET7499123.1"
                     /translation="MNRSRSLRAFLLAATLLAAGASPARATFSIVARDPETGDLGVAV
                     QSHYFSVGPIVPWAEPGVGAVATQSLVEVAYGPRGLALMRDGATAKDALKKLLDDDQY
                     REVRQVAMVDSHGDVAVHTGKKCIPFAGDEAGDHFTCEANLMASDKVWPAMAAAYRSA
                     KGDFPERLLQALEAGQRAGGDIRGQQSAAIVIVKGARSDKPWADRVMDLRVEDSPKPI
                     AELRRLVGLWRAYKKSDEGDAFITENKVEDALRAYSEAARLAPGNDELLFWQAATLWK
                     LGREKEAAPLFRKVFAKDRRWVTLAPRLVPADLLPDDPASIRRIQALAPPARARAR"
     gene            5283..6458
                     /locus_tag="VFM00_12565"
     CDS             5283..6458
                     /locus_tag="VFM00_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015128627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET7499124.1"
                     /translation="MSERTTSTPAAAASPVPYIPGLDGVPAARSSICWIDGDQGVLEY
                     RGYPIETLAEKSTFEETSYLLLWGKLPNRAELEKFSRDLRSNRDVRFRLIDLLKCLPD
                     TGHPMEALQASIAALGMFYPDRDPMKPEDRYNSSVRLIAKAPTLVAAFHRLSLGKDYI
                     PPREDLSHAANFLYMLTGDVPEEDEARVMDICFILHAEHSMNASTFGGRVTASTLADP
                     YTVISSAIGALTGPLHGGANEQVIHMLQQIGDAERARPYIEDLIHRKQKIMGIGHRIY
                     RVKDPRALVLQRLAGQMISKGGGDPLLAVAQEVEKVTAEHLGAKRIYPNVDFYSGVLY
                     TAMGLPAALFTPMFAIARVSGWLAHWLEQIQDNRIYRPDQIYTGTHAEPYIPMEERV"
     gene            6487..7323
                     /locus_tag="VFM00_12570"
     CDS             6487..7323
                     /locus_tag="VFM00_12570"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HET7499125.1"
                     /translation="MCTLIAGVEVLGPRTLVLGANRDESPERASAPPGILRERPRVVG
                     GRDLVAGGTWLAVRDARAVTALMNRRPTPGTAPPAGGPAGEAPLEGGPAPRSRGLLCL
                     NAAALGGAQDQPHRIDPGTGERHPARLHAALALIDRDAYAACTLVGLNVDEPSWAILA
                     EAGQETGVRILEPGWNVVTHAEVNDPGEPRTAWLRSRLESLAPRTADEALRAIAALLV
                     THEDVERSVPGVCLHRDRFPTVSSSLIALGDLGNRGPQRYLHAPGPPCSTPYADLSSL
                     LT"
     gene            7405..7791
                     /locus_tag="VFM00_12575"
     CDS             7405..7791
                     /locus_tag="VFM00_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7499126.1"
                     /translation="MRKRIGMTAAAAVAVAAAVAIAAAWRERAPSGPVETVTLSMKDY
                     TFNGQNPTLRFSPGERVRFVVSNDEDTRVLHNFRIVGLDVPCGPPLLPGERRVVTVTM
                     PRSPGEFVYTCCTHPGMGGKLVIAER"
     gene            complement(7838..>8160)
                     /locus_tag="VFM00_12580"
     CDS             complement(7838..>8160)
                     /locus_tag="VFM00_12580"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HET7499127.1"
                     /translation="SLWGYDPDRNQYQCVWTDEMSSAASVFTGSFTDDKTLFLSSSTV
                     VDGKPITEIMTLKFDEPGKYSLVMTSDAAGDPKPIMEEAATKSGGPVEKASEKKEVSD
                     KNNG"
BASE COUNT         1232 a         2862 c         2830 g         1236 t
ORIGIN      
        1 tgcccgtcat cgtgttcgtc gcgcgcgtgc cgttcaggat gaggagcgcc cgggtggtgt
       61 ccacggtgac cgtgtgctcc cggttgaacg ccgtcgtgta ggtgttcacg agcaccgggt
      121 gctcgggatc ggtcatccgg atgatctgga tgccgccgcc gatggcctcg gtcgaaacgt
      181 agagccaggt ccggtactgc ttcatctcgc gccaccagga ggtgaggccc gggatgaagc
      241 ccacctcgta gggcgccgag gggttcgtca cgttgaggat cgaggtgccg gtctcggtgc
      301 cgagcacggc atattcgcgc ccgtccggcg ccacgtaggc ggtgcacgcg gaatagcgcg
      361 tgtagttgct cacatgggcg aggagctgga cgtttcggga cacggccctg gccatggccg
      421 gagccggccg tagcggcgcg agggcggcca cggcaacgag caccccaaga aggaagtttc
      481 ggcgacacgc ggacatggac tggtttcgga tgcgggggat gggatggggg ttcacgggaa
      541 aaggggctcc gagcgggtgc cggcctggac gcgcccgccc ggtgattcta ccgtcgtcag
      601 gcccccggcg ccagccctac agccagccga cctcgcgata ccactccgcc gtcgcccgga
      661 ttccctcgta aagcccgaca cgaggcgtcc atccgagacg ctcccggatc ttccgggacg
      721 agcagatcca atcccccgcc gcgatctccg ccagcttttc gcgggagaga aggggcgcct
      781 tgcccgcggc gcgggcccag ctctcggcga ccgccgcgat ccccgccagc gtccactggg
      841 gaatggcgac ccggaccgtc caggtgcgga gcgcctcgcg gacggaacgg cccagctcgt
      901 cgtagtcggt cacgtccggc tcgcaggcga agaacacctg ccccaccgcg gcctcctgcg
      961 tgagcgcggc atggaccgcg gtgacgaggt cgctgacgtg gacgatggag aaccgccctc
     1021 gcttccggag aaccggctcc aggtgccact tccccgcgac gaacgccttg agaatcgcgg
     1081 tgtcgcgcgg gccgtagacg gccggggggc ggatcaccga gacgggcagc cggtccgccg
     1141 ccatcaccac gagctgctcc gcgcgaagct tgctctcgcc gtaggcggag accgggtggg
     1201 gacgctgatc ctcggtaacg ggaaggccgg tcaggctggg gcccgcggcc gcctggctcg
     1261 agatgaagag gaaacgccga agggtgcccc cgtccgcggc gcgcaggagc cgctcggtgc
     1321 ccacgacgtt cgcctcgtga aactccgccg cgttccgcgc gcggatgagc ccggcggcgt
     1381 ggacgatcca gtcggccccc tccaccgcgc gggccagggc gccgggatcg tcgagcgcgg
     1441 catcggccag ctcgaccggc ttctgctcca gccaccgccg cgacgaggag ccgcgcacga
     1501 cggcacgcac gcgcgcgccg cgcagaagga gctcgtccac gatgtggctt ccgacgaagc
     1561 cggaagcgcc ggtgacgacg gcgaggggcg ccgcgccggt cacgcgagcc gcttttcgta
     1621 gatccggtag atcgcgctcc gcgtgccgcc cgagccgagg atcagctgat gcacggagtc
     1681 gttgtcctcc agcgtccatc cgacttcggt catcgcgtac ccgcggcgac gggtcgcgcg
     1741 catcagcgcc tcgacgagga gcgctccgac gccgcgcctc tggtattcgc cgagcacgcc
     1801 cagcgccagc aggcggcctt ccttgatccg cggcagcgtc cagagcagct tcacgatgcc
     1861 gaacgggaag agcttcccct tcaccttggc cagcgcgacg ttcacgtcgg gaaggatcag
     1921 cccgaatcca accggcgcgc cgtccacctc ggcgaactgt accaggtcgg ggaccaccgc
     1981 gggcttcagc tggctcgcca tgtgatcgat ctcggcgtcg gtcatcggaa cgaagcccca
     2041 gttccgttcc caggccgcgt tgtagacggt cttcacacgg cccagctcct cgttgaactt
     2101 cttcatctcg aggggccgga ccgtgatccg gtcacgcttc ctggccatcg acgcgatccg
     2161 ctggaatacc ggcggcggcg tctcgggcac ggggacgcgc cactcgtaga ggtccttggc
     2221 cttggcgagc cccgcagctt cgatccacac ggcgtactcg cgaacgttgt aaggcatgag
     2281 gagcatcgga cggtgatcga aaccttcgat caggagcccg cattcgtcgt tggtggtgaa
     2341 gctgaggggg ccgcgcagca cctgggcgcc cttttcacgc ccccaccgct cggcggcgac
     2401 caggagcgcc gccgtcgcgg ccggcgaatc ggcctcgaag aagccgaacg cggcctgccg
     2461 ctccccctgg aaggcgtcgt ggttgcgatc cacggtcgcg gcgatcctcc cgacgacgcg
     2521 cccgccgtcg agcgccagga aataggcggc ctcggcgtgc tcgaagtagg gattcttacc
     2581 gggggtgagg cgcgctttct cgtcggaggg gagcggaggg acccagtgcg gctcgttccg
     2641 gtagcggtcg tagacgtagc ggatgaagcg atccaggtcc ttcgcggttt ggaccggaac
     2701 gacctccacg ccgggctcgg tgcggactat tcggcctggg aggccagctc ggcgccgagc
     2761 tgctggcgga cctcggagac gacgcgaaga atggtgtcga gctgggagtc ctggtgcgtc
     2821 gccatgtacg aggtgcggag cagatcgcgt ccgggtgcca cggcggggac caggatcgcg
     2881 ttcacgaaca cgccccggtc gtagatcttc cgccaccagt gcatgcactt catgcggtcg
     2941 ccgataaaga tcgggatgat cggcgtgccc ttgcccgagc cgacttcgag tccctgggcc
     3001 ttgaacccgg tccgcatctt gtccgcgatg acgcgcaggc gctcgcggag gaccggaccc
     3061 tcgtcgctct cgatcacgtc gagggccgcg agcgccgcgg ccacgctcga gggcgtcatg
     3121 ctcgcgctga agatcatggc gcgcgcgtga tggcgcaggt agtccatgac ctccttgggg
     3181 ccggcgatga agccgccgag cgacgcgaac gacttgctga aggtgcccat cacgagatcg
     3241 acctggtcct cgacgttctg cgcctcgcag gcaccggcgc ctcgggcgcc gagcacgccg
     3301 atcccgtgcg cgtcgtccac caggagccgc gcgttgtact cgcgcaccag gcgcacgatg
     3361 tcgggaaggg gcgcgatgtc cccctccatg ctgtacaccc cgtccacgac gaccagcttg
     3421 cccgccttgg ccggcacctt ctccagcacg cgctccaggt cgcgcaggtc gttgtgccgg
     3481 aagcggagca cctgcccgtg cgagaggcgg gccccgtcca cgatgcaggc gtgcgcgtcc
     3541 ttgtccagga taaccacgtc gcccttctcg gcgagcgcgg agatcgaacc caggttggtc
     3601 aggtagccgg tcgagtagac cagcgcggct tccttcttca tgaacttggc gagccgctcc
     3661 tcgaggatcg cgtgcagctc gagcgtgccg ttcaggaagc gggagccgtt gttcgaggtc
     3721 ccgaagcgca tcagcgcgtc ttcggcggcg gccatgacct tgggatgctt ggtgagcccg
     3781 aggtagttgt tcgacccggc catgatcatg cgcttgccgt ggatcgtgac ctcggtgccg
     3841 tgattttcgg agatcgggag gaagtacggg tagaagccgg ccctctgggc catccggtgt
     3901 tcgttgaact cgttacactt ggcgaagatg tccacgcgca cctttggtgt tggggttcag
     3961 tgatggtcgc gagccgccgg agggccattt ggcgaatcgc gggacacctt agcggttccg
     4021 gccccgggag tcaatcggcg gagggccgat ccgcgggccg gccgccccgc cgccccggta
     4081 tctccttgcc gcatgggcac taatcgccga tgatccggcc ggcggcgctc ccgccggggc
     4141 atgacccggc cggcacggcc gcctcgaact gcccacccca cccgatgaaa ggagacgccg
     4201 tgaaccggtc ccgctccctc cgagcgttcc tcctggccgc gacgctgctc gccgccggcg
     4261 cttccccggc acgggccacc ttttcgatcg tggcccgcga tccggaaacc ggggacctgg
     4321 gcgtcgccgt ccagtcgcac tacttctcgg tcggcccgat cgtcccctgg gcggagccgg
     4381 gcgtgggcgc cgtggcgacg cagtcgctgg tcgaggtggc gtacggcccg cgcgggctgg
     4441 ccctgatgcg cgacggggcg acggcgaagg acgcgctgaa gaagctcctc gatgacgacc
     4501 agtaccgcga ggtgcgccag gtggcgatgg tggattccca tggcgacgtc gccgtgcaca
     4561 ccggcaagaa gtgcatcccc ttcgccgggg acgaggccgg cgaccacttc acgtgcgagg
     4621 ccaacctcat ggccagcgac aaggtctggc ccgccatggc cgcggcctac cggagcgcca
     4681 agggggattt ccccgagcgg ctgctccagg ccctggaggc cgggcagcgc gccggcggcg
     4741 acatccgcgg gcagcagtcg gccgcgatcg tcatcgtgaa aggcgcccgc tccgacaagc
     4801 cctgggccga ccgggtcatg gatctccgcg tggaggacag ccccaagccg atcgccgagc
     4861 tgcgccggct ggtcggcctg tggcgcgcgt acaagaagag cgacgaaggg gacgccttca
     4921 tcaccgagaa caaggtggag gacgcgctcc gcgcctactc cgaggcggcg cggctcgcgc
     4981 cggggaacga cgagctcctc ttctggcagg cggccaccct ctggaagctg gggcgcgaga
     5041 aggaagcggc cccgctcttc cgtaaagtct tcgccaagga ccgccgatgg gtgaccctgg
     5101 caccgcggct ggtgcccgcc gacctgctcc cggacgatcc cgcgtccatt cggcgcatcc
     5161 aagctcttgc gcccccggcg cgggcccggg cgcgatagaa tggctcgatc gagcccgtag
     5221 cccgccgtac cgaaaccctc accggaccac gccccggctc gccgaggcat cggaggaacg
     5281 tcatgtcgga acgcaccacc tcgacgccgg cggcggccgc ttcgccggtc ccctacatcc
     5341 ccggcctcga cggagtcccc gcggcgcgct cgagcatctg ctggatcgac ggggaccagg
     5401 gcgtgctcga ataccgcggc taccccatcg agacgctcgc ggagaagagc acgttcgagg
     5461 agacctccta cctgctccta tggggaaagc tgccgaaccg ggccgagttg gagaagttct
     5521 cgcgcgatct gcgctccaac cgcgacgttc gcttccggct gatcgacctc ctgaagtgcc
     5581 tgcccgacac cggccatccg atggaggcgc tgcaggcctc gatcgcggcg ctcgggatgt
     5641 tctatcccga ccgcgacccg atgaagcccg aggaccggta caactcctcc gtgcggctga
     5701 tcgccaaggc accgacgctg gtggcggcct tccaccgcct gagcctggga aaggactaca
     5761 tcccgccccg cgaggatctg tcgcacgcgg cgaacttcct ctacatgctg accggcgacg
     5821 tccccgagga ggacgaggcg cgggtcatgg acatctgctt catcctccat gccgagcact
     5881 cgatgaacgc ctcaacgttc gggggccggg tcaccgcctc gaccctggcc gatccctaca
     5941 ccgtgatctc ctcggcgatc ggcgcgctca cggggccgct gcacggcggc gcgaacgagc
     6001 aggtgatcca catgctccag cagatcggcg acgccgagag ggcgcgcccc tacatcgagg
     6061 acctgatcca ccggaagcag aagatcatgg ggatcggaca ccggatctat cgcgtgaagg
     6121 atccgcgggc gctcgtgctg cagcggctgg ccgggcagat gatctccaag ggaggcggcg
     6181 atccgctgct cgcggtggcg caggaggtgg agaaggtgac cgccgagcat ctcggcgcga
     6241 agcggatcta tccgaacgtg gacttctact ccggcgtgct ctacacggcg atggggcttc
     6301 ccgccgcgct cttcacgccg atgttcgcga tcgcgcgcgt ctccggatgg ctcgcccact
     6361 ggctggagca gatccaggac aaccgcatct accggcccga ccagatctac accggcacgc
     6421 acgccgagcc gtacattccg atggaggagc gcgtctaggc ggaaggaccg acggttcgag
     6481 cgcggcatgt gcacgctgat cgcgggggtc gaggtactcg gcccccgcac gctcgttctg
     6541 ggcgccaacc gcgacgagtc ccccgagcgc gcttccgcgc ctcccggcat tctgcgcgag
     6601 cggccgcgcg tggtaggcgg cagggacctg gtggcgggcg gcacctggct cgccgtgcgc
     6661 gacgcgcggg cggtcaccgc gctgatgaac cggcgcccaa cgccgggaac agcaccgccc
     6721 gcgggcgggc cggcgggaga ggcgccactc gagggcgggc cggcgccgcg ctcgcgcggg
     6781 ctcctctgtc tcaatgccgc ggcgttgggt ggcgcgcagg accaacccca tcgcatcgat
     6841 ccgggcacgg gtgagcgcca tccggcgcgg ctccacgccg cgctcgccct gatcgaccgg
     6901 gacgcgtatg ccgcctgcac cctggtgggc ctgaacgtgg acgaaccctc gtgggccatc
     6961 ctcgccgaag cggggcagga gaccggggtc cggatcctcg agccgggctg gaacgtggtg
     7021 acgcacgccg aggtgaacga tccgggcgag ccgcgcacgg cctggctccg gagccggctg
     7081 gagagcctgg cgccgcgcac cgcggatgag gcgctgcggg cgatcgcggc gctcttggtc
     7141 acgcacgaag acgtcgagcg ctccgtcccc ggcgtctgcc tccatcgcga ccgcttcccc
     7201 accgtctcga gctcgctgat cgcgctcggg gatctcggga accggggccc gcagcgctac
     7261 ctccacgcgc cggggccgcc ctgctccacg ccctacgccg acctctcgtc gctccttacg
     7321 tgacccgggc aggcgtgccc ccggcaggac tgacgtgagg cgggcggctt gtccccgacg
     7381 ggaccgccgt gtcacgattc ggtcatgcgg aagcgcatcg ggatgacggc ggcggccgcg
     7441 gtggcggtcg ccgcggcggt ggccatcgcc gcagcctggc gtgagcgcgc gccgtcgggt
     7501 ccggtcgaga ccgtcaccct cagcatgaag gactacacgt tcaacggcca gaaccccacg
     7561 ctgcgctttt ctccggggga gcgcgtccgc ttcgtcgtga gcaacgacga ggacacgcgc
     7621 gtgctgcaca acttccggat cgtgggcctg gacgtgccct gcggtccgcc cctcctgccg
     7681 ggcgagcgcc gcgtggtcac cgtcaccatg ccacgctcgc ccggcgaatt cgtctacacc
     7741 tgctgcaccc accccgggat gggcggcaag ctggtgatcg cggagcgctg accggctttc
     7801 gctggcgtgg ccggcctcag cccggcgctg accggcctca gccgttgttc ttgtccgaga
     7861 cctctttctt ctcggaagcc ttctccaccg gcccgccgct cttcgtggcg gcctcctcca
     7921 tgatcggctt gggatcgccc gcggcgtcgc tcgtcatgac gaggctgtac ttgcccggtt
     7981 cgtcgaactt gagggtcatg atctcggtga tcggcttccc gtccaccacc gtcgacgaag
     8041 agaggaagag cgtcttgtcg tccgtgaagg agccggtgaa caccgacgct gccgaggaca
     8101 tctcgtcggt ccagacgcac tggtactgat tccggtccgg gtcgtagccc cagaggctcc
//
LOCUS       DASSLZ010000335         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1385482, whole genome shotgun sequence.
ACCESSION   DASSLZ010000335 DASSLZ010000000
VERSION     DASSLZ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385482"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFM00_12585"
     CDS             complement(<1..209)
                     /locus_tag="VFM00_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499128.1"
                     /translation="MKRDEGSGRASALLIAALAAALAAALASAPALAQNGDIEVERTV
                     SKVEIHGNASVGDGTLKKLLRTRGRG"
     gene            complement(220..1596)
                     /gene="rseP"
                     /locus_tag="VFM00_12590"
     CDS             complement(220..1596)
                     /gene="rseP"
                     /locus_tag="VFM00_12590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET7499129.1"
                     /translation="MMAAFLPTLLYGALVLGVLVFVHELGHFLVAKWLGVRVLSFSIG
                     MGPRLFGFRAGETDYRISLLPLGGFVRMAGDNPEEEREGDAGEFLEKPWWARALITVA
                     GPLANLIFAMLLFFGLYTIGYTESDYPARVGRVEANSTAARVGLRSGDAFLTWGGVPA
                     RTMGQLRDAIENGMSKKSSGPMAFQVQRGGQVVALNVPREQVSDIPSGVQWDMKTVIG
                     QVLIGNPAYAAGIREGDRILAVDGKPVANWNDLQEKVTKRPDQDIALTIGREKQVFRA
                     TVHTTPEGKIGIGPVEQISVRRTVPPVTAAKYAVLKTVETVGLIYTGLWSFVSNPVKL
                     HNTIAGPIAIAQVANQQASGGFDQLVLFAAFISLALMAMNLLPIPILDGGHVFFALIE
                     GVRKRPLSLKTQLFFQRIGLIVLVGLVVFSFYNDLNRVSQRRRAEADISKRLTVPAAG
                     DSVGRATP"
     gene            complement(1661..>2436)
                     /locus_tag="VFM00_12595"
     CDS             complement(1661..>2436)
                     /locus_tag="VFM00_12595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7499130.1"
                     /translation="LLYGLPIEAIAAISEGVRRALETSGVPGEVIRVIPSGIDLEPFE
                     QPFDRAAARASLGLTPDEVLAIQVAALAPHKSQTTLLQAAAAAAGAAPGLRVWIAGEG
                     ALKEALLAEHRSLGLGDRVRFLGFREDIVPLLRAADLFVLSSYLEGLGTSVLDAMAAG
                     LPVVATAVGGVPEIVEPEATGLLVPPQDPGALAAAMARLATDPALRAAMGARGRERAR
                     AFSADRTAVLTRALYEELLDRLPGSRGRTTGRPEVSGPP"
BASE COUNT          385 a          843 c          855 g          353 t
ORIGIN      
        1 ccgcgccccc gggtgcgcag gagcttcttc agcgtcccgt cgccgaccga agcgttgccg
       61 tggatctcga ccttgctgac ggtgcgctcg acttcgatgt cgccgttctg ggcgagggcc
      121 ggcgcggatg ccagagcggc ggcgagggcg gccgcgagcg cggcgatgag gagggccgaa
      181 gcccttcccg agccctcgtc gcgcttcaag ggagcgcggt cagggcgtcg cgcggcccac
      241 gctgtctccc gccgccggga ccgtgagtcg cttggagatg tcggcctccg cgcgccgcct
      301 ttgggagacg cggttcaggt cattgtagaa ggagaagacc acgagcccga cgagcacgat
      361 caatccgatg cgctggaaga agagctgggt cttcaaggag agggggcgct tccgcacgcc
      421 ctcgatcagg gcgaagaaga cgtgcccgcc gtccaggatc gggatcggca gcaggttcat
      481 cgccatgagg gcgaggctga tgaaggcggc gaagaggacg agctggtcga agcccccgct
      541 cgcctgctgg ttcgccacct gcgcgatggc gatcggcccc gcgatggtgt tgtgcagctt
      601 gaccggattc gagacgaagc tccagagccc cgtgtagatg agccccaccg tctccaccgt
      661 cttcagcacg gcgtatttgg ccgcggtgac cggcggcacg gtgcgccgga ccgagatctg
      721 ctccaccgga ccgatgccga tcttcccctc cggcgtcgtg tgcacggtcg cccggaacac
      781 ctgcttctcg cggccgatcg tgagcgcgat gtcctggtcg ggacgcttcg tcaccttctc
      841 ctggaggtcg ttccagttcg cgaccggttt cccgtccacg gccaggatgc ggtccccttc
      901 gcggatgccg gcggcgtagg ccggattccc gatgagcacc tgcccgatca ccgtcttcat
      961 gtcccactgg acgccgctcg ggatgtctga gacctgctcg cgcggcacgt tcaacgcgac
     1021 cacctggccg ccccgttgca cctggaacgc catggggccg gagctcttct tcgacatccc
     1081 gttctcgatg gcgtcgcgga gttgccccat cgtgcgggcc gggacgccgc cccaggtcag
     1141 aaaggcgtcg ccgctccgga gcccgaccct ggccgccgtc gagttcgcct ccacgcgtcc
     1201 gacccgggcg gggtagtccg actccgtgta gccgatggtg tagaggccga agaagaggag
     1261 catcgcgaag atcaggttcg cgagcgggcc cgcgaccgtg atcagcgcgc gcgcccacca
     1321 cggcttttcg aggaactcgc cggcgtcccc ctcccgctcc tcctcgggat tgtctcccgc
     1381 catccggacg aagccgccga gcggaagcag cgagatccgg taatcggtct ctccggcccg
     1441 gaacccgaag agacgcggcc ccatgccgat ggagaacgag aggacgcgca cgccgagcca
     1501 cttggccacc aggaagtggc ccagctcgtg gacgaagacg agaacgccca ggacgagggc
     1561 tccgtacagc agggtcggca ggaaggcggc catcatgatc ctcggtcgga cgcgaatggg
     1621 aacgggctcg gcaacacggt aacaggttgg gaattttgaa tcaaggcggc ccggacacct
     1681 ccgggcggcc cgtcgtacgc cccctcgaac cagggagccg gtccaggagt tcctcgtaaa
     1741 gcgctctcgt gaggacggcc gtccgatcgg ccgagaacgc acgggcccgt tcccttccac
     1801 gggcccccat cgcggcgcgc agcgcggggt cggttgcaag ccgcgccatg gccgcggcga
     1861 gggcgccggg gtcttggggc ggcacgagca gtccggtcgc ttcgggttcc acgatctcgg
     1921 ggacgccccc cacggcggtc gcgacgaccg gcagcccggc ggccatggcg tcgagcacgg
     1981 acgtgcccag tccctcgagg taggacgaca gaacgaagag gtcggccgcg cgcaggagcg
     2041 gcacgatgtc ctcccggaag cccaggaagc gcacgcgatc gcccagcccg agcgatcggt
     2101 gctccgcgag gagcgcctcc ttgagcgcgc cctctcccgc gatccagacg cggagccctg
     2161 gagccgcgcc ggccgcggcc gccgccgcct ggagcagcgt ggtctgcgac ttgtgcggtg
     2221 ccaacgccgc gacctgaatc gcgagcactt cgtccggagt aaggccgagc gaggcgcgcg
     2281 cggcggcgcg atcgaaaggc tgctcgaacg gctcgagatc gatcccgctt ggaatgacgc
     2341 ggatgacctc gccggggacg ccgctcgtct cgagggcccg ccggactccc tcggagatgg
     2401 cggcgatcgc ctcgatcgga agtccgtaga gaagcc
//
LOCUS       DASSLZ010000336        12172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_234685, whole genome shotgun sequence.
ACCESSION   DASSLZ010000336 DASSLZ010000000
VERSION     DASSLZ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12172
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234685"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFM00_12600"
     CDS             <1..535
                     /locus_tag="VFM00_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499131.1"
                     /translation="AFVLFHSGIVAEQTVAKMEAQGKDPRAIEQVQSFFEGTGGLIVT
                     VLTAPAATAVGLLSGAAILFFMANLMMGARLRFAHYLSAVAFAGIVQLVDQLVRVGLI
                     LANRSFDVRLGLGMLFGDSTGFGVRALDTLTDPLFLWGNAIGALAVGTYSKRGFGFGV
                     LATLPGVILAVILSANR"
     gene            689..2089
                     /locus_tag="VFM00_12605"
     CDS             689..2089
                     /locus_tag="VFM00_12605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7499132.1"
                     /translation="MLALILTAPLAAPARAQTPADTTGFNHFGVDSLPPPVAVGERWS
                     LARCIETALRQNGDVRVAQARTRQASGSALSAWSGILPSLDVSGSRSRFWPDKKNNTQ
                     LVPVQQGDSTVLVEVSLNRRDQTTLDATAQTNLISVPSWMEKRRRDRLHEGARWSEAE
                     TRNTVAFRVKQQYFNLLKAERLAAVSRESELLARDEERRSEALFEVGTVARGDVLKAR
                     ARRAQTQLDRIRAYNQVQIQTARLRQVIGLPPDASIAVEPILEEGVAVPDSASAVRTA
                     LERRPRLEGAHATERAARAGVTGAWGVRLPQLTASYFVSHAKTTNDFQVGGFPSQEEI
                     SGTDRSGELRLSMPIFDGLAIEGNIRQAKGVLAEAEANRRQLELDVAVEVQSAWLALR
                     EAVERIDVAREGLASAEEDYNFSKSRYELGAGTFLDLLNAEVSLAQARQSLVEAQADA
                     RTAEADLERAIGERRY"
     gene            2142..3428
                     /locus_tag="VFM00_12610"
     CDS             2142..3428
                     /locus_tag="VFM00_12610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7499133.1"
                     /translation="MKAKTRTILLVSGGAVLLAAIVVANLARGGGAKTAVQAADVKRG
                     KIASTVRAPGRIQPVTQVKLSANVPGEVVRLAVKEGDVVRKGQFLLQLDDTQVRARMR
                     ESEAALEASRSNLRLAEASLAQSDASLQRKEALFVRKLVSPEEVEAARTQRNADKARV
                     DANREDISRAMATLAGARDNLRKTSFYAPIPGTVTQLAIERGEIVVTGTMNNPGTVIL
                     TIADMSAMKVEADVDETDVSSVALGQPVTVKVDAFPDLTLHGSVVEIANSPKISESGT
                     QEQQTNFEVDVSIQNPPASLRPGMTADVEIRTAEKDGVLTVPIQSVVVRTPEELKPAV
                     KGRRAKAKNAAAAAAPADSAGKPGEEIKGVFVIVNGAAQFRKVTPGITSDTDMEVAGD
                     VKPGEKVITGPFRVLRNLKPEARVKIEEPKRPQEKK"
     gene            3500..4192
                     /locus_tag="VFM00_12615"
     CDS             3500..4192
                     /locus_tag="VFM00_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7499134.1"
                     /translation="MGADEVHALDGVSLDIAKGEYVAVMGPSGSGKSTLMNVIGCLDT
                     PTSGSYQLRGTEIREREDDELARIRNQEIGFIFQTFNLLPRADALHNVELPLIYSGTP
                     AKERRDRARDLLAQVGLADRMHHRPNELSGGQRQRVAIARALVNRPSIVLADEPTGNL
                     DSKTGVEIMALLAEINGRGNTVILVTHEEDIAAHARRVIRLRDGLIESDRAQAPRPAV
                     PHAVPAAPAAPS"
     gene            4189..5442
                     /locus_tag="VFM00_12620"
     CDS             4189..5442
                     /locus_tag="VFM00_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7499135.1"
                     /translation="MTGPARSRREFWAAQGENLKIALDAIRANKLRSVLTVLGNVVAV
                     MSVIMVVAVIDGVNTYVGEKILEQGLQVVYIDKFGLITNDDAFREAEKRRDLTLREAR
                     ALETRMRLASRVVAQVESTKRVRAGRNELANVTVRGTTAGYEEVGNFEVVEGRHLDPS
                     DVERRRPVAVVGSEVANALYPRVDPIGQRLKVAGQELLVIGVLKPKGNVLGQSQDNIV
                     LTTLGQYDKMFGAHRSLTITVKAQPGVPLERVEDEARVVLRSERHVPLGKPDDFGIST
                     AETFRALYQSLTSGIFAGTIALVAISLVVGGIVIMNIMLVAVTERTREIGLRKALGAR
                     RSDLTGQFLSEAVVLSTFGGVLGVAAGISFALFIRAVTPLPASIQPWSVLISLGVASS
                     VGLFFGVYPATRAAQLDPIAALRQE"
     gene            5446..6675
                     /locus_tag="VFM00_12625"
     CDS             5446..6675
                     /locus_tag="VFM00_12625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7499136.1"
                     /translation="MAIDVREGVTIAFRAIRSNKLRSFLTVLGVIIGITTIMAMVSII
                     EGLNRSMKAQLASIGTDVLYIRTFAPGAFFGGFPDSLRHRPWFKPEDAQAIRNQADAV
                     LAVSPLNFTQGVKLRYGETETRANAVVGSSPDYLVTNGYAVVNGRYFTDAEVEHRSPV
                     CVLGLDQVEALFPHGTSVGKSIYIGGRPFTVIGELERRGKFVGMSLDDIVITPYTSLE
                     KNFGPDLPMVLNAKPKAPELIDTAKDQITEVLRRQRRLRYNQSDNFAVFTDQSLVDLY
                     KQITGAFYLVMVVISSIGLLVGGIGVMNIMLVSVTERTREIGIRKAIGARQKDVLWQF
                     LVEAMTLTGTGGIIGVTLGLLVGWLIDVLTPLSFAVPPWGILLAFFSSTSIGLFFGMY
                     PAVKAARLDPVEALRYE"
     gene            6691..9567
                     /gene="uvrA"
                     /locus_tag="VFM00_12630"
     CDS             6691..9567
                     /gene="uvrA"
                     /locus_tag="VFM00_12630"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7499137.1"
                     /translation="MDLIKIRGAREHNLKSLDLDIPRNRLVVITGVSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLGQMEKPDVDQIEGLSPAISIEQRTAGTNPRSTVATVT
                     EIYDYLRLLYARIGVQHCVECGRPIRRQTVQEMVDHLLAAYRGRRLQILAPVVRGRKG
                     EYRKEIAQWRRLGYVRARVDGGWVELEEEIALDKKRKHTIEIVVDRLPADTERRSRLH
                     EALETATRLAEGLALALVDGDEEETLSSSAACPACGISYEPLQPRSFSFNSPYGACKT
                     CDGLGTTMEIDPDLVVPDRAKSLREGAIPMWGDAEGTWTKGTLKAVAKRLKISLDTPF
                     GKLPASAKNALLSGLGDEKLEYAFRLKDGRTLSHTGKFRGILNELQRRYRETTSDAVR
                     EQIGALMTKKPCPACGGARLKPESLAVKVDGLSIAEWTGRPVTRALEAMRGMTLSPRD
                     AGIAAPVLKELRQRLEFLDDVGVGYLTLDRAAGTLSGGEAQRIRLATQIGSRLTGVLY
                     ILDEPSIGLHPVDNKKLLETLLALRDLGNSVLVVEHDRETMDAADHIVDLGPGAGRRG
                     GYLVAEGTVEEVRAVPGSLTGQYLSGAREIPIPAERRAGSGAALEVVGARQNNLRGIR
                     ARFPLGTLVCVTGVSGSGKSTLVNDILYKALARRFYQAKEPPGAHEKILGLEHVDKVV
                     AIDQSPIGRTPRSNPATYTGLFTFIRDLFAATPEAKVRGYGPGRFSFNVKGGRCEACG
                     GDGLCTIEMHFLPDVYVTCEVCRGKRYNRETLEVTYKGRSIADVLEMTVEDALELLGA
                     VPPIRRKLETLAGVGLGYIHLGQAATTLSGGEAQRVKLASELSRVETGRTLYLLDEPT
                     TGLHFEDVRQLLDVLDRLVDRGNTVIVIEHNGDVMARADWILDLGPGGGEAGGRVVAE
                     GTPEAVAANPESKTGEALREFFRSQGRAVEAPSGRVRRRREAVV"
     gene            9630..10763
                     /gene="gcvT"
                     /locus_tag="VFM00_12635"
     CDS             9630..10763
                     /gene="gcvT"
                     /locus_tag="VFM00_12635"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET7499138.1"
                     /translation="MLDTAAALRRTPFHPRHVALGAKMVPFAGYEMPIQYEGIVAEHR
                     TVRSTVGVFDVSHMGEIRLTGPGAVAYADRLVTNETRSLPLGKVAYSPMCREDGGIVD
                     DLLVYALGKERLLVVNAANFDKDLAWVQAHGPADVSVVDENAVTAQLAVQGPRAEAVI
                     AKLYGDRAVALAFYEAYETGEGEDRTLVSRTGYTGEDGFEIYVRPSRALDLWDRVFEA
                     GAPEGIRAIGLGARDTLRLEMGYCLYGNDIDETTNPIEAGLAWTVRLDKEDFIGRGAI
                     ACVKEEGPKRKLLGLLAEGDRIPRHGFAVEQDGRPAGTVTSGSMGISLGVPVAMAYLD
                     AAAAKAGTRVQVQVRGQAVPAVVTSRPMYTKGTVRSPKPRRSA"
     gene            10760..11146
                     /gene="gcvH"
                     /locus_tag="VFM00_12640"
     CDS             10760..11146
                     /gene="gcvH"
                     /locus_tag="VFM00_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET7499139.1"
                     /translation="MKVPGELRYTKEHEWVRVKGNEAEVGITDYAQGELGDVIFVELP
                     QTGSKLAHMKPFGTIDAVKTVSDLFAPVSGEVAAVNESLQTNPALVNQSPYENGWMVR
                     IRMENPGEVDKLLSADEYEKLLGAHA"
     gene            11143..>12172
                     /gene="gcvPA"
                     /locus_tag="VFM00_12645"
     CDS             11143..>12172
                     /gene="gcvPA"
                     /locus_tag="VFM00_12645"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET7499140.1"
                     /translation="MSFVGRSDDERRAMLERIGASKIEELWGAIPEPFRVTGPLALPA
                     PMAEYEIARRMEQLAGENADATRYACFLGAGIYDHFVPSALRAITGRSEFATAYTPYQ
                     PEVAQGTLQVIFEYQSMIRELTGMEVANASMYDGATAAAEAAHLAAGATGRRRVLVSA
                     GLHPHARRVLATYASAGNFDLEVLPLEGGRTPAAAVARSAGSGPGLAALLWAQPNFEG
                     VVEDGRALTEAAHAAGAYSVASADPVALAVLSPPGEDGADVVVGEGQPLGVSMSYGGP
                     LVGFFAIRKKDVRRLPGRLAGLTVDLDGKKGYVLTLQTREQHIRRERATSNICTNQGL
                     MALANTIHL"
BASE COUNT         1935 a         4139 c         4315 g         1783 t
ORIGIN      
        1 cgctttcgtg ctcttccatt ccggcatcgt ggccgagcag accgtggcca agatggaggc
       61 tcagggcaag gatccgcgtg ccatcgagca ggtgcagagc ttcttcgagg gcaccggggg
      121 tctgatcgtc accgtcctca ccgcgcccgc cgccacggcg gtcggcttgc tgtcgggggc
      181 ggcgatcctc ttcttcatgg ccaatctgat gatgggcgcg cggctccgct tcgcgcacta
      241 tctctcggcc gtggcgttcg ccggcatcgt gcagctcgtg gatcagctgg tgcgcgtcgg
      301 cctgatcctg gcgaatcgtt ccttcgacgt gcgcctgggc ctggggatgc tcttcgggga
      361 ctctacggga ttcggcgtgc gcgccctcga cacgctcacc gatccgctct tcctgtgggg
      421 gaatgccatc ggcgccctgg ccgtcgggac gtactcgaag cgcggcttcg gattcggcgt
      481 cctggcgacc cttcccggcg tgatcctcgc cgtgatcctc tcggcgaacc ggtgatcgcc
      541 catccccatg aaacggaagg agacctccgg acgtactgaa ggagtccggg gacccgccat
      601 catccgcgcc accgtctcga gggggatagg aatgccgtcg tcatccgttt cggctcgtgc
      661 ccgcgggggg ttccgacccg cgttcctgtt gctcgcgctc atcctcacag caccgctcgc
      721 ggcgcccgcc cgcgcccaga cccccgccga caccacgggg ttcaatcatt tcggcgtcga
      781 ctcgctcccg ccgccggtcg cggtcggcga gcgctggagc ctggcccgct gcatcgagac
      841 ggccctccgc cagaacggcg acgtccgcgt ggcgcaagcg cgcacgcggc aggcgagcgg
      901 ctcggcgctc tcggcctgga gcggcattct gccgtccctc gacgtcagtg gctcgcggag
      961 ccgcttctgg ccggacaaga agaacaacac gcagctcgtt cccgtccagc agggggattc
     1021 gacggttctc gtcgaggtgt cgctgaatcg cagggaccag acgacactgg acgcgaccgc
     1081 acagacgaat ctgatcagcg tgccgagctg gatggagaag cggcgccgcg accgtctgca
     1141 cgagggggcg cgctggagtg aggccgagac ccggaacacg gtagccttcc gtgtgaagca
     1201 gcagtacttc aaccttctca aagccgaacg gctggcggcc gtgtcccgcg agtcggagct
     1261 attggcacgg gacgaagagc gccggtcgga agcgttgttc gaggtgggga ccgtggcgcg
     1321 gggcgacgtt ctcaaagctc gggcgcgccg cgcgcagacc cagctcgacc gtattcgagc
     1381 gtacaatcag gtgcagatcc agaccgcgcg cctgaggcag gtgatcgggt tgccgcccga
     1441 cgcatcgatc gccgtcgagc cgatcctcga ggagggggtg gccgtgcccg attccgcgtc
     1501 cgccgtccgg accgccctcg agcggcgtcc acggctggag ggcgcgcacg cgaccgagcg
     1561 tgccgcccgg gcgggcgtga ccggcgcctg gggcgttcgt ctgccgcagc tcacggcctc
     1621 ctacttcgtg tcgcacgcga agaccacgaa cgacttccag gtcggcggat tccccagcca
     1681 ggaggagatc tccggaaccg accgctcggg cgagcttcgc ctgtcgatgc cgatcttcga
     1741 cgggcttgcg atcgagggga acatccggca ggccaagggg gttctcgccg aagcggaggc
     1801 gaatcgccgc cagctcgagc tggacgtcgc tgtcgaagtg cagtcggcgt ggctcgcgct
     1861 gcgcgaggcg gtggagcgga tcgacgtggc gcgggaaggg ctggcgtcgg ccgaggagga
     1921 ctacaacttc tccaagagcc gttacgagct gggggcggga acgttcctcg acctcctgaa
     1981 cgccgaggtg agcctggcgc aggcgcggca gagcctggtc gaggcgcagg ccgacgcccg
     2041 cacggccgaa gccgacctcg agcgcgcgat cggcgagcgg cggtactagg ccgcgcggcg
     2101 cgcggcggcc cgcggcgtag acccccgata cccggaggcg catgaaggcg aagacccgaa
     2161 ccatcctgct cgtgtccggc ggggccgtgc tgctcgccgc gatcgtcgtc gcgaacctgg
     2221 cgcgcggcgg cggcgccaag accgccgtgc aggccgccga cgtgaagcgc gggaagatcg
     2281 cctccaccgt ccgcgccccc ggacggatcc agccggtgac gcaggtcaag ctctccgcga
     2341 acgttccggg cgaggtcgtg cgcctcgccg tcaaggaagg ggacgtggtg cgcaaggggc
     2401 agttcctgct ccagctggac gatacgcagg tgcgggcgcg gatgcgcgaa tcggaggccg
     2461 ccctcgaggc ctcgcggtcc aatctccggc tggccgaggc ctcgctcgcg cagtccgacg
     2521 cctcgctgca gcgcaaggaa gccctcttcg tgcgcaagct ggtctctccg gaggaagtgg
     2581 aagccgcgcg cacccagcgc aacgccgaca aggcgcgggt ggacgccaat cgcgaggaca
     2641 tctcccgcgc gatggccacg ctggccggcg cgcgcgacaa cctgcgcaag acctccttct
     2701 acgcgccgat tccgggcacg gtcacccagc tcgcgatcga gcgcggcgag atcgtggtca
     2761 cgggcacgat gaacaacccg ggcaccgtga tcctcacgat cgccgacatg agcgcgatga
     2821 aggtcgaggc cgacgtggat gagaccgacg tgagctccgt ggcgctgggg cagccggtca
     2881 ccgtcaaggt ggacgccttc cccgacctga cgctccacgg cagcgtggtc gagatcgcga
     2941 actcgcccaa gatctccgag tccggcacgc aggagcagca gacgaacttc gaggtcgacg
     3001 tgtcgatcca gaaccccccc gcgtccctgc ggccggggat gaccgccgac gtcgagatcc
     3061 gcacggccga gaaggacggc gtcctcaccg tgccgatcca gtcggtggtg gtgcgcacgc
     3121 ccgaggagct gaagcctgcc gtgaaggggc gccgcgcgaa ggcgaagaat gccgccgcgg
     3181 cggccgcgcc ggccgattcg gcggggaagc ccggcgagga gatcaagggc gtgttcgtca
     3241 tcgtgaacgg cgccgcccag ttccggaagg tgacgccggg catcaccagc gacaccgaca
     3301 tggaggtcgc cggcgacgtg aagcccggcg agaaggtgat caccggcccg ttccgcgtgc
     3361 ttcggaatct caagcccgag gcccgggtga agatcgaaga gccgaagcgt ccccaggaga
     3421 agaagtgacc ggccccgagc cctcgcgcgc cgacggcgcg cccctcatcc ggatccgaga
     3481 cctgcgcaag atctacgtga tgggcgcgga cgaggtgcac gcgctggacg gcgtctccct
     3541 ggacatcgcg aagggcgagt acgtcgcggt gatgggccct tcgggatcgg ggaagtccac
     3601 cctcatgaac gtgatcggct gcctcgacac cccgaccagc ggctcctacc agctccgcgg
     3661 caccgagatc cgcgagcggg aggatgacga gctcgcgcgg atccggaacc aggagatcgg
     3721 attcatcttc cagaccttca acctgctccc gcgcgccgac gccctgcaca acgtggagct
     3781 gccgctcatc tattcgggca ccccggccaa ggagcggcgc gatcgggcgc gcgatctcct
     3841 ggcccaggtc ggactggccg atcgcatgca ccatcgcccc aacgagctct cgggcgggca
     3901 gcggcagcgg gtcgcgatcg cccgcgccct cgtgaaccgc ccctcgatcg tgctcgccga
     3961 cgagccgacc ggaaatctcg actcgaagac gggggtcgag atcatggcgc tgctggccga
     4021 gatcaacggc cgcgggaaca cggtcatcct cgtcactcac gaagaggaca tcgccgccca
     4081 cgcgcggcgc gtcatccggc tccgcgacgg cctgatcgag agcgaccgcg cgcaggcgcc
     4141 ccgtccggcc gtaccgcacg ccgtcccggc cgctcccgcc gcgccgtcgt gaccgggccc
     4201 gcgcgcagcc gccgcgagtt ctgggccgcg cagggcgaga atctcaagat cgcgctggac
     4261 gcgatccgcg ccaacaagct ccggtcggtc ctcaccgttc tcggcaacgt cgtggcggtc
     4321 atgtcggtca tcatggtggt cgcggtgatc gacggcgtga acacgtacgt gggcgagaag
     4381 atcctcgagc agggcctcca ggtcgtctac atcgacaagt tcggcctgat caccaacgac
     4441 gacgcgtttc gggaagcgga gaagcggcgg gacctgacgc ttcgcgaagc ccgggccctg
     4501 gagacgcgga tgcgtctcgc gtcccgcgtc gtcgctcagg tggagtccac caagcgggtc
     4561 cgcgccggac ggaacgagct cgcgaacgtg accgtccgcg gcacgacggc cgggtacgag
     4621 gaggtgggaa acttcgaggt ggtggagggc cggcacctgg atccctccga cgtggagcgg
     4681 cggcgccccg tcgccgtcgt ggggtccgag gtcgcgaacg ccctctatcc gcgcgtggac
     4741 ccgatcggac agcggctcaa ggtcgcgggg caggagctcc tggtgatcgg ggtgctgaaa
     4801 ccgaagggaa acgtgctggg ccagagccag gacaacatcg tcctgacgac gctggggcaa
     4861 tacgacaaga tgttcggtgc ccatcgatcc ctcacgatca cggtcaaagc ccaaccggga
     4921 gtccccctgg agcgtgtcga ggacgaggcg cgcgtcgtcc tccgctccga acggcacgtc
     4981 ccgctcggca agccggacga tttcggcatc tcgacggcgg agaccttccg cgcgctctac
     5041 cagtcgctga cgagcggaat cttcgccggg acgatcgcgc tcgtggcgat ctccctcgtg
     5101 gtgggcggca tcgtgatcat gaacatcatg ctggtcgcgg tcacggagcg tacccgggag
     5161 atcgggctcc gcaaggcgct gggggcgcgc cggtcggatc tgaccggcca gttcctgagc
     5221 gaggcggtcg tgctctccac gttcgggggc gtcctgggcg tggcggcggg aatttccttt
     5281 gccctcttca tccgcgcggt gaccccgctc ccggcctcca tccagccctg gtcggtcctg
     5341 atcagcctgg gcgtcgcctc gtccgtgggg ctcttcttcg gcgtctaccc ggcgacccgc
     5401 gcggcccagc tcgacccgat cgcggcgctc cggcaggaat aggcgatggc gatcgacgtt
     5461 cgcgaagggg tgacgatcgc gttccgcgcg atccgctcga acaagctccg ctccttcctg
     5521 accgtgctcg gcgtgatcat cgggatcacc acgatcatgg cgatggtctc gatcatcgag
     5581 gggctgaacc ggagcatgaa ggcgcagctc gcctccattg gcaccgacgt gctctacatc
     5641 cggacgttcg cgccgggggc cttctttgga gggttcccgg acagcctgcg ccaccggccc
     5701 tggttcaagc cggaggacgc ccaggcgatc cggaaccagg ccgacgcggt gctggcggtg
     5761 tctcccctca acttcacgca gggcgtgaag ctgcgctacg gcgagaccga gacccgggcg
     5821 aacgccgtcg tgggctcgag ccccgactat ctggtgacca acggctacgc ggtcgtgaac
     5881 ggacgctact tcaccgacgc cgaggtggag catcgctcgc cggtgtgcgt cctggggctg
     5941 gaccaggtgg aagctctctt cccgcacggc acgtcggtgg ggaagagcat ctacatcggc
     6001 ggacgcccct tcaccgtgat cggcgagctg gaacggcgtg gcaagttcgt gggcatgagc
     6061 ctcgacgaca tcgtaatcac cccctacacc tcgctggaga agaacttcgg ccccgacctg
     6121 ccgatggtgc tgaacgccaa gcccaaggca ccggagctga tcgacacggc caaggatcag
     6181 atcaccgagg tgctccgccg ccagcggcgg ctgcgctaca accagtcgga caacttcgcg
     6241 gtcttcaccg accagtcgct ggtggatctc tacaagcaga tcaccggcgc gttctatctg
     6301 gtcatggtcg tgatctcctc gatcgggctc ctggtggggg ggatcggggt catgaacatc
     6361 atgctggtct cggtcacgga gcggacccgg gagatcggga tccggaaagc gatcggcgcg
     6421 cgccagaagg acgtgctctg gcagttcctg gtggaggcga tgaccctgac cggaacgggg
     6481 gggatcatcg gggtgacgct ggggctcctc gtgggctggc tgatcgacgt cctgaccccg
     6541 ctctccttcg cggtgccgcc gtgggggatc ctcctcgcct tcttctcctc gacttcgatc
     6601 gggctcttct tcggcatgta cccggccgtc aaggcggccc ggctcgaccc cgtggaggcg
     6661 ctgcgctacg agtagggtag aatctccccc atggacctca tcaagattcg cggcgcccgg
     6721 gagcacaatc tcaaatccct cgacctcgac atcccccgga accggctggt ggtgatcacc
     6781 ggcgtctccg gctcgggaaa gtcctcgctc gccttcgaca ccctctacgc cgaggggcag
     6841 cggcgctacg tcgagtcgct ctcggcctac gcgcggcagt tcctggggca gatggagaag
     6901 cccgacgtgg accagatcga ggggctctcg ccggccatct cgatcgagca gcgcacggcg
     6961 ggaacgaacc cgcgctccac ggtggccacg gtcacggaga tctacgacta cctgcgcctc
     7021 ctctacgcgc ggatcggcgt gcagcactgc gtggaatgcg gtcggcccat ccgccgccag
     7081 acggtgcagg agatggtgga ccatcttctc gccgcctacc gcggccggag gctccagatc
     7141 ctggcgccgg tcgtgcgcgg gcggaagggg gagtaccgga aagagatcgc gcagtggcgg
     7201 cggctgggct acgtgcgggc gcgcgtggac ggcgggtggg tggagctcga ggaggagatc
     7261 gccctcgaca agaagcggaa gcacacgatc gagatcgtcg tggaccgcct gccggccgac
     7321 acggagcggc ggagccgcct ccacgaggcg ctggagaccg cgacccgtct ggcggaaggg
     7381 ctcgcgctcg ccctggtgga cggcgacgag gaggagacgc tctcctccag cgcggcctgc
     7441 ccggcgtgcg gcatctccta cgagccgctc cagccccgga gcttctcctt caacagtccc
     7501 tacggcgcgt gcaagacgtg cgacggactg ggcaccacga tggagatcga tcctgacctg
     7561 gtcgtgcccg accgggccaa gtcgctgcgc gagggggcga tccccatgtg gggcgacgcc
     7621 gaggggacct ggaccaaggg gacgctcaag gccgtggcca agcggctgaa gatctcgctg
     7681 gatacgccct tcggaaagct gcccgcctcg gcgaagaacg cgctgttgag cgggctgggg
     7741 gacgagaagc tggagtacgc cttccgcctg aaggacgggc ggacgctgtc gcacacgggg
     7801 aagttcaggg gaattctgaa cgagctgcag cgccgctatc gcgagaccac gtcggacgcg
     7861 gtgcgggagc agatcggcgc cctcatgacc aagaagccct gccccgcctg cggcggcgcg
     7921 cggctgaagc cggagtcgct ggccgtgaag gtggacgggc tctcgatcgc ggaatggacg
     7981 ggccggccgg tcacgcgcgc gctcgaggcg atgcgcggga tgacgctctc gccgcgcgac
     8041 gccggcatcg cggccccggt gctcaaggag ctgcgccagc ggctggagtt cctggacgac
     8101 gtcggggtcg gctacctcac gctggaccgc gcggcgggaa cgctctcggg gggcgaggcg
     8161 cagcggatcc gcctcgcgac ccagatcggg tcgcgcctga ccggggtcct ctacatcctg
     8221 gacgagccga gcatcgggct tcatcccgtg gacaacaaaa agttactcga gacgctgctc
     8281 gccctgcgcg acctcgggaa ttccgttctc gtcgtggagc acgatcgtga aaccatggac
     8341 gccgccgacc atatcgtgga cctcggcccg ggcgccgggc gacggggcgg ctatcttgtg
     8401 gctgagggta cggtggagga agtccgagcc gtgccggggt cccttacggg acagtatctc
     8461 tccggcgcgc gcgagatccc gatccccgcg gagcggcgcg ccggaagcgg cgccgcgctc
     8521 gaggttgtcg gggcgcggca gaacaacctg cgcgggatcc gcgcccgctt cccgctcggc
     8581 accctggtct gcgtgaccgg cgtctccgga tcggggaaga gcacgctcgt gaacgacatc
     8641 ctctacaagg cgctcgcgcg ccgcttctac caggccaagg agccgccggg cgcgcacgag
     8701 aagatcctgg ggctggagca cgtggacaag gtcgtggcga tcgaccagtc gcccatcggc
     8761 cggacgccgc gctcgaaccc cgcgacctac accggcctgt tcacgttcat ccgcgacctc
     8821 ttcgccgcca cccccgaagc gaaggtgcgc ggctacgggc cggggcgctt ctcgttcaac
     8881 gtgaaggggg gccgctgcga ggcgtgcggc ggcgacgggc tctgcacgat cgagatgcac
     8941 ttcctgccgg acgtctacgt cacctgcgag gtctgccgcg gcaagcgcta caaccgggag
     9001 acgctggagg tcacgtacaa gggacgctcg atcgccgacg tgctcgagat gacggtggag
     9061 gacgcgctgg agctgctcgg cgcggtgccg ccgatccgcc ggaagctcga gacgctcgcc
     9121 ggggtggggc tgggctacat ccacctgggg caggcggcga cgaccctctc ggggggcgag
     9181 gcgcagcggg tgaagctcgc ctcggagctc tcgcgggtgg agacgggacg gacgctctac
     9241 ctcctcgacg agccgaccac ggggcttcac ttcgaggacg tgcggcagct cctcgacgtt
     9301 ttggaccgcc tcgtcgaccg ggggaacacc gtgatcgtga tcgagcacaa cggcgacgtg
     9361 atggcccgcg ccgactggat cctggacctg ggtccgggcg ggggcgaggc ggggggcagg
     9421 gtcgtggccg aggggacgcc ggaggcggtc gcggccaacc cggaatcgaa gacgggagaa
     9481 gcccttcgcg agttcttccg gagccagggg agggccgtgg aggcgccgtc gggacgggtc
     9541 cggcgacgcc gggaggcggt cgtttgaaat cccgccggga tgtgctaagg tagctcgtcc
     9601 gcgcgcgcaa cgaccataaa tagacgagga tgctcgatac cgccgccgcg ctccggcgca
     9661 ctccatttca tccgcgacac gttgccctgg gagccaagat ggtcccgttc gcagggtatg
     9721 agatgcccat tcagtacgag ggcatcgtcg ccgagcaccg cacggttcgt tccaccgtcg
     9781 gggtcttcga cgtctcccac atgggtgaga tccggctgac gggccccggc gccgtcgcct
     9841 acgccgaccg cctggtcacc aacgagaccc gctcccttcc cctcggcaag gtcgcctact
     9901 cgcccatgtg ccgcgaggac ggtggcatcg tggacgatct cctggtctac gccctcggca
     9961 aggaacggct cctggtcgtc aacgccgcca atttcgacaa ggatctcgcc tgggtgcagg
    10021 cgcacgggcc ggcggacgtg tccgtcgtgg atgagaacgc cgtcaccgcc cagctcgccg
    10081 tgcagggtcc gcgagccgag gcggtgatcg ccaaactcta cggcgaccgc gccgtcgcgc
    10141 tcgccttcta cgaggcgtac gagacgggcg agggagagga ccggaccctg gtctcgcgca
    10201 ccggctacac cggcgaggac ggcttcgaga tctacgtgcg cccctcgcgc gcgctcgacc
    10261 tctgggaccg cgtgttcgag gcgggcgctc cggaggggat ccgtgccatc gggctgggcg
    10321 cgcgtgacac gctgcggctg gagatggggt attgcctgta cggaaacgat atcgatgaga
    10381 ccacgaatcc gatcgaggcc gggctggcgt ggacggtccg tctcgacaag gaggatttca
    10441 tcggccgggg cgcgatcgcg tgcgtgaagg aggagggccc gaagcgaaag ctcctcgggc
    10501 tgctcgccga gggcgaccgc atcccgcgcc acggcttcgc ggtggagcag gacggacgtc
    10561 ccgcgggaac ggtgaccagc ggctcgatgg ggatctccct cggcgtcccg gtcgcgatgg
    10621 cgtacctgga tgccgccgcc gcaaaggcgg ggacgcgggt tcaggtccag gtccgcggcc
    10681 aggcggtgcc ggccgtcgtc accagccgtc ccatgtacac gaagggaacc gtccgctcgc
    10741 ccaaacccag gagatcggca tgaaggtgcc cggagagctt cggtacacca aggagcacga
    10801 gtgggtgcgc gtgaagggga acgaggccga ggtcgggatc accgactacg cccagggtga
    10861 gctcggggac gtgatcttcg tcgagctgcc ccagaccggt tcgaagctcg cgcacatgaa
    10921 gccgttcggc acgatcgacg ccgtgaagac ggtgagcgat ctcttcgccc ccgtctccgg
    10981 cgaggtggcc gcggtgaatg aatcgctcca gaccaatccc gcgctcgtga accagtcgcc
    11041 ctacgagaac ggctggatgg tccggatccg gatggagaat ccgggcgagg tcgacaagct
    11101 cctttccgcc gacgaatacg agaagctcct cggagcgcac gcgtgagctt cgtcggccgc
    11161 agcgacgacg agcgccgggc catgctggag cggatcggcg cctcgaagat cgaagagctg
    11221 tggggcgcca tccccgagcc cttccgcgtc acggggccgc tcgccctccc ggcgccgatg
    11281 gccgaatacg agatcgcgcg ccgcatggag cagctcgccg gcgagaacgc cgacgcgacc
    11341 cgctacgcct gcttcctggg cgccggcatc tacgaccact tcgttccctc cgcccttcgc
    11401 gcgatcacgg ggcggagcga gttcgcgacc gcctacaccc cctaccagcc cgaggtcgcg
    11461 caggggacgc tccaggtcat cttcgaatac cagtcgatga tccgcgagct gaccggcatg
    11521 gaggtggcga acgcctcgat gtacgacggg gcgaccgccg ccgccgaagc ggcccatctc
    11581 gccgccggcg ccacggggcg ccgccgcgtg ctcgtgtcgg ccgggctcca tccccacgcg
    11641 cgccgcgtgc tcgcgacgta cgcgtcggcg gggaacttcg atctcgaggt gctcccgctc
    11701 gaaggcggcc gcacgccggc cgccgcggtg gcgcggagcg ccggctccgg tccgggtctg
    11761 gccgccctcc tctgggcgca gcccaacttc gagggggtcg tcgaggacgg acgcgccctg
    11821 accgaagccg cgcacgcggc gggcgcctac tcggtcgcct ccgccgatcc cgtggccctc
    11881 gcggtgctct cgcccccggg cgaggatggg gccgacgtgg tcgttggcga ggggcagccg
    11941 ctgggcgtct cgatgtccta cggcggcccg ctggtcggct tcttcgccat ccggaagaag
    12001 gacgtgcgcc ggcttccggg gcggctggcc gggctgaccg tggatctcga cggcaagaag
    12061 ggctacgtgc tcacgctcca gacccgcgag cagcacatcc ggcgcgagcg ggcgacctcg
    12121 aacatctgca cgaaccaggg gctgatggcc ctcgcgaaca cgatccacct gg
//
LOCUS       DASSLZ010000337        11099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1387266, whole genome shotgun sequence.
ACCESSION   DASSLZ010000337 DASSLZ010000000
VERSION     DASSLZ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11099
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387266"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFM00_12650"
     CDS             complement(<1..353)
                     /locus_tag="VFM00_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005162450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HET7499141.1"
                     /translation="MSAFEPPLLARHYTRFRVAERLLLTGHSHQAWPDVALEGQLEAW
                     NDAAELADEKWGRAAGKADRVRRGFARLLGGAADEVALGQNTHELVLRFLSALPLVDR
                     PRLVSTDGEFHSIRR"
     gene            complement(394..1176)
                     /locus_tag="VFM00_12655"
     CDS             complement(394..1176)
                     /locus_tag="VFM00_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HET7499142.1"
                     /translation="MGVTYGSYLKLDELLALQSPLSEGPEHDETLFIVIHQVYELWFK
                     ELLHELDYLKKCLAANDLPRAQHTLKRVLTILKVVVAQIDVLETMTPLEFLSFRARLE
                     SGSGFQSWQFRELEFLLGARSDAALARYPEGSAARERLARRASEPTVWDAFLRFLASA
                     GYDVPLELLGRDVTRAVAPAPELHRVLIEVYRKDAMTASLCERLVDLDEGFQEWRYRH
                     VKMVERTIGTKQGTGGSEGAAYLRTTLDKPFFPDLWAIRSEL"
     gene            complement(1188..3440)
                     /locus_tag="VFM00_12660"
     CDS             complement(1188..3440)
                     /locus_tag="VFM00_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HET7499143.1"
                     /translation="MKAAVIGGGPAGLFFALLLKRADPNHEIAVYERNRPDDTFGFGV
                     VFSDATEEALAVADADVAAAMSARCHRWDDIEVHYKGHTLVSKGHRFSGLSRAGLLGI
                     LAGRCREAGVRLCFDREVADPASLEGADLILAADGVNSAVRERYREHFRPVVDVRPNR
                     FVWLGTTKPFPAFTFYFKSDASGLWRVHAYQYEPDRSTFIVEATEATWRRSGLAEGDE
                     ARTIAFCEALFAEELEGHRLLGNRSIWRSFPTIRNGRWSHGNVVLAGDAAHTAHFSVG
                     SGTKLAMEDAVALVDALRNEPSIPDALAAYEAARRPPVESLQRAAQASLQWFEDTERY
                     MDLEPLQFAFTLITRSLRVTHENLKLRDPAFTARVDSWFAEKAEEQSGAPVPRTPAPP
                     PMFTPFRLRDLVLPNRVVVSPMCQYSAVDGMPEDWHLVHIGSRAVGGAGLVFVEMTDV
                     SPEGRISPGCTGLYSEKHAAAWKRIVDFVHGRTPAKIAMQLGHAGRKGSTKLSWEGDA
                     EPLDKGGWPILAPSPIPYFPHSQTPRAMTRADMDTVIGQFTQAAHHAVTAGFDLLEIH
                     MAHGYLLASFISPLTNLREDEYGGSLENRMRYPLEIFDAVRAVWPEEKPMSVRISAVD
                     WKPGGMGPEDAVAVARMLRGHGCDIVDVSAGQTVPDQRPVYGRLFQTPFADRIRHEAT
                     IATMAVGNISSYADVNTILAAGRADLCLLARAHLWDPYWTRHAAYELGYRLSWPDPYE
                     SLNQFRPRFS"
     gene            complement(3437..4582)
                     /locus_tag="VFM00_12665"
     CDS             complement(3437..4582)
                     /locus_tag="VFM00_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7499144.1"
                     /translation="MIPDPRPIRALLDPSHETFAAEAAAFAAREIALRTPAATDAAAR
                     TEARAILAALGAGRMLEPIRRGDWRACCLAREALAAASPLADAVFALQGLGTLPLLIT
                     GSAAGARLAEEAIAGRAMSSFAMTETEAGSDVASLRTRARKDGGDYVLDGAKSYISNA
                     GIADFYVVFASTDPDARGKGISAFLVPAGAPGLRFAGAQVLSAPHPLGELMFEGCRIP
                     AANLLGEEGRGFALGLKTLDRMRATVGAAACGMAARAMQEAAAHARARTQFGKPLAEF
                     QLVQEKIARMATELDAARLLVYRAAWEADHGAERVTMEAAMAKSFATEAAQRIVDDAV
                     QVLGARGVMADHPVDRLYRAVRALRIYEGTTEIQHLVIAGQVLQAGR"
     gene            complement(4579..5409)
                     /locus_tag="VFM00_12670"
     CDS             complement(4579..5409)
                     /locus_tag="VFM00_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HET7499145.1"
                     /translation="MSQEPRPADLTPLQPKSLLYAVDEATNVATITLNRPERLNALTF
                     EVYAELRDLFAALDQEEGVRAIVLTGAGRGFCSGGDVEEIIGALFERDFGGLREFTRM
                     TCDLIRNIRGCRKPVIAALNGTTAGAGAVIATACDLRIASETAKIAFLFVRVGLSGAD
                     MGASWLLPRIVGLGRATELLMTGDFIDAKRAYEIGLYHRVTAPDRVLAEATAYAERLA
                     RGPSAALAVTKEALDYEFSQDLVSALNYESDAQAALMEHPNFREAYEAFREKREPRFR
                     "
     gene            complement(5406..5828)
                     /locus_tag="VFM00_12675"
     CDS             complement(5406..5828)
                     /locus_tag="VFM00_12675"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7499146.1"
                     /translation="MTFEIVNPRSLGEPKGWSNGLLAPAGGRLLFVAGQAGWEDEASG
                     DVPDFAAQFVRALDKVLSVIAEAGGRPQDLARLTIYVTDLAAYRAARRALGEAWRARL
                     GRFYPAVALLEVKGLMDRGAMVEMEATAVIAGPSGGGA"
     gene            complement(5825..6607)
                     /locus_tag="VFM00_12680"
     CDS             complement(5825..6607)
                     /locus_tag="VFM00_12680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7499147.1"
                     /translation="MADRPLDGRVALVTGGGRGIGAAIARLLAKSGAAVGVAARSRAE
                     CDAVASEIRSEGGTASGYSCDVTNEEEVRELSTRVRIELGNPEVLVMSAGAAMAAPFR
                     EVTISDWDAMMNANARSTFLCASAFVPTMAAHGFGRVVAVASVAGLTGGKYIAPYAAS
                     KHAVVGLVRSLAAELEGKGVTVNAVCPGYVDTSITHKAVEGAMSRGGLSHPEALAAIL
                     GTTGQERLLTPEEVARAVLKLIVDPQHPTGQALVLGARVHAA"
     gene            complement(6600..7355)
                     /locus_tag="VFM00_12685"
     CDS             complement(6600..7355)
                     /locus_tag="VFM00_12685"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET7499148.1"
                     /translation="MAPRKWADLTWEEVRDLDRHRAVALLPVGAIEAHGPHLPLATDV
                     ILAEAMAVAAAARLEAEGRIAVVLPPLPYAAAPFGAGFPGTISVGAASVKAVLLDLAR
                     ELTRQGFAALGVANAHLDPAHLETLSAARAAADEEELLPVICPDLTRKPWALRLGEEF
                     QTGACHAGRYEGSVVLAVRPDLVREETRRALAPNPSSLSRAIRAGARTFEDAGGPRAY
                     FGWPADATAEEGMRTIEALGAILAEAVLERHHG"
     gene            7428..8948
                     /locus_tag="VFM00_12690"
     CDS             7428..8948
                     /locus_tag="VFM00_12690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HET7499149.1"
                     /translation="MTYEPPERLNLADHLLDARVREGRGGRTALVTDQGSLTYADVQA
                     LANRFGHLIREAGAEPEQRVLIALPDGPEFVAALFGTLKIGAVAVMVNPGLSLEEIRH
                     LLAYTRARVAVAHRDTAAAFERAAGEAPLVKRLLTAGGDPFARALAAAPAALEAFPSH
                     RDDPAIWLFSGGTTGRPKAVVQTHRSFAYTTACYGPEVIGYTENDVTLSVPKLYFGYA
                     TGANLFFPFAAGGASALFPEPATAETVFAKIRKFRPTVLVNVPTMVHKMVTHPGASAE
                     DLASLRVAISAGEALPAELYERWKKTTGVELLDGLGTAEMWHIFISNRIGAVRPGTLG
                     TVVPGFEIQVCDEEGRELPPRETGWLRVRGGARAIGYWQHQEKTEQAFRGEWYVTGDL
                     VQRDTDGTVTYCGRGDELLKVSGKWLAPAEVEGCLLDHPAVAEAAVVGAADGSGLVKP
                     IAYVVARERREGLADELRAHVRERLAPYKHPREVVFLDALPRTHLGKVDRGRLRAG"
     gene            8954..9748
                     /locus_tag="VFM00_12695"
     CDS             8954..9748
                     /locus_tag="VFM00_12695"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7499150.1"
                     /translation="MTPARGGPRGGRGALLVGTSGWAYRHWPRFYPPDLRPPRYLAHY
                     ATRFPTVEVNTSFYHLPLRTTYAKWAAETGPDFRFALKLSRYVTHIKRLSGVKAETRL
                     FLERAEPLGKKLGPILVQLPPQLRADPIRLERFLAMVRSLEQERGERVRLAFEFRHET
                     WFASKEALDALARHGAALVFGQSGRYPYPSAEPLTASWVYLRFHGPREFCSSQYGREG
                     LAPWGEKIARWRAEGRDVYAYFNNDVHGYAVEDAARLVRMATADQA"
     gene            complement(9824..10354)
                     /locus_tag="VFM00_12700"
     CDS             complement(9824..10354)
                     /locus_tag="VFM00_12700"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET7499151.1"
                     /translation="MSAPWVGARKRSSSRDRTRIIGGGQGASRELARHASAWDVHCRT
                     RHEGSWAWKRERGSRPSNRRRPMPTRKRKSSGRRYGESASKSVESAMRRRKKGTLKSG
                     KSGHGGKVTSRKQAIAIGLSEARAKGAKVPSPKKRSSTRSRRSTSGRSTRSSSRRSTS
                     RSSSSRARSSSRSARS"
     gene            10353..>11099
                     /locus_tag="VFM00_12705"
     CDS             10353..>11099
                     /locus_tag="VFM00_12705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET7499152.1"
                     /translation="MPRRAASARRSPSAPRRSPSRQKQAPRANRSVRSATSAASRLLD
                     GCFNVADLEVIAKRKLPRPTYDYYAGGAEDERTLARNRAGFERYALLHRVLVDVERVD
                     TSTTVLGAAVTNPVLVAPTAYQRLADDQGETATARAAGQAGSLMTVSTLATRSLEEVA
                     AAATGPLWFQLYVYRDRAVTERLIARAEAAGYRALVVTVDTPRLGRRERDHRNGFTLP
                     RGLSIANFTEDADRAGLERYGSLAAYASAQL"
BASE COUNT         1596 a         4041 c         3882 g         1580 t
ORIGIN      
        1 tggcggcgga tcgagtggaa ctcgccgtcg gtgctgacca gcctcggtcg atcgaccagg
       61 gggagcgcgg agagaaagcg cagcaccagc tcgtgggtgt tctgccccag agcgacctcg
      121 tcggccgcgc cgcccaagag ccgggcgaag cctcggcgca cgcggtcggc cttgcccgcc
      181 gcgcgccccc acttctcgtc cgccagctcc gccgcgtcgt tccacgcctc gagctgtccc
      241 tcgagcgcga cgtcgggcca ggcctggtgc gagtgtccgg tcagcagtag ccgctcggcg
      301 acgcggaagc gggtgtagtg ccgggccagc aacggcggtt cgaaagcgct cacgctcgtc
      361 tggccctcgc ttggcgtcgg cttcgccgac gcgtcatagc tccgaccgga tcgcccacag
      421 gtccgggaag aacggcttgt ccagggtcgt ccgcaagtag gccgccccct ccgagccgcc
      481 ggtcccctgc ttcgtgccga tcgtgcgctc caccatcttc acgtggcggt agcgccattc
      541 ctgaaagccc tcgtccagat ccacgagccg ctcgcacaag gacgcggtca tcgcgtcctt
      601 ccggtagacc tcgatcagca cgcggtgcag ctccggcgcg ggggcgaccg cccgggtcac
      661 gtcgcgcccc aacagctcca gcggcacgtc gtagcccgcc gacgccagga atcggaggaa
      721 ggcgtcccac accgtcggct ccgacgcgcg ccgcgccagc cgctcgcgcg cggcgctccc
      781 ctcggggtag cgcgcgagcg cggcgtcgct cctcgcgccg agcaggaatt ccagctcccg
      841 gaactgccac gactggaatc cgctccccga ctccagccgc gcgcggaagg agaggaactc
      901 cagcggcgtc atcgtttcga gcacgtcgat ctgggccaca accaccttca ggatcgtgag
      961 cacccgcttc aacgtgtgct gcgcccgggg cagatcattc gcggcgaggc atttcttgag
     1021 gtagtccagc tcgtggagca gctccttgaa ccagagctcg tagacctggt ggatgacgat
     1081 gaagagcgtc tcgtcgtgct cgggcccctc ggagagcggg gactgcagcg cgagaagctc
     1141 gtccagcttg agatagctgc cgtaggtgac tcccatcgcg cctccgatca ggaaaagcgg
     1201 ggacggaact ggttcagcga ttcgtaggga tccggccagg aaagccggta gcccagctcg
     1261 taggcggcat ggcgcgtcca gtaggggtcc cacaggtgcg cccgcgccag caggcagagg
     1321 tcggcgcgcc ccgcggccag gatcgtgttc acgtcggcgt aggaggagat gttccccacc
     1381 gccatcgtcg cgatcgtggc ctcgtgccgg atccgatcgg cgaagggggt ctggaagagc
     1441 cgtccgtaga ccggccgctg gtcgggcacg gtctgccccg ccgagacgtc tacgatatcg
     1501 cacccatgtc cgcggagcat ccgcgccacg gcgacggcgt cctcgggacc catccctccg
     1561 ggcttccagt ccaccgcgga gatgcggacc gacatcggct tctcctctgg ccagaccgcg
     1621 cgcaccgcgt caaagatctc gagcgggtag cgcatgcggt tctccagaga tcccccgtac
     1681 tcgtcctcgc ggaggttcgt gagcggcgag atgaagctcg cgagcaggta gccgtgcgcc
     1741 atgtggatct cgagaagatc gaatccggcc gtcaccgcgt ggtgcgccgc ctgggtgaat
     1801 tgcccgatca ccgtgtccat gtcggcgcgg gtcatcgcgc gcggcgtctg gctgtgcgga
     1861 aaatagggga tcggcgaggg agcgaggatc ggccagccgc ccttatcgag cggctccgcg
     1921 tccccttccc acgagagctt ggtcgagccc ttccttcccg cgtggcccag ctgcatcgcg
     1981 atcttcgccg gcgttcgccc gtgcacgaag tccacgatcc gcttccaggc cgccgcatgc
     2041 ttctcggaat agaggcccgt gcacccaggg gagatccgcc cctcggggct cacgtcggtc
     2101 atctccacga agacgagccc ggcgccgccg accgcgcgcg atccgatgtg caccaggtgc
     2161 cagtcctcgg gcatgccgtc gaccgccgag tactggcaca tcggcgagac gacgacgcgg
     2221 ttcggcagca ccaggtcgcg caggcggaag ggggtgaaca tcggcggcgg cgcgggcgtg
     2281 cgcgggacgg gagcgcccga ctgctcctcc gccttctccg cgaaccacga gtccacgcgc
     2341 gcggtgaacg ccggatcccg aagcttcaag ttctcgtgcg tcacgcgcag gctccgggtg
     2401 atcagcgtga acgcgaactg cagcggttcc aggtccatgt agcgctcggt gtcctcgaac
     2461 cactggagcg aggcctgggc cgcgcgctgc aggctctcca ccggcggacg gcgcgcggcc
     2521 tcgtaggccg cgagcgcgtc ggggatggag ggctcgttcc ggagcgcgtc gaccagcgcc
     2581 acggcgtcct ccatcgcgag cttggttccg gagcccaccg agaagtgggc ggtgtgcgcc
     2641 gcgtctcccg cgagcacgac gttgccgtgc gaccagcgcc cgttccggat ggtcggaaag
     2701 ctccgccaga tcgagcggtt cccgagaagg cggtgcccct ccagctcctc ggcgaagagc
     2761 gcctcgcaga acgcgatcgt gcgcgcctcg tcaccctcgg cgaggccgga ccggcgccac
     2821 gtcgcctccg tggcttcgac gatgaacgtg gagcggtcgg gctcgtactg gtaggcgtgc
     2881 acgcgccaga gcccgctcgc gtcgctcttg aaatagaagg tgaacgccgg aaagggcttc
     2941 gtggtgccaa gccagacgaa ccggttgggg cggacgtcca cgacggggcg gaagtgctcg
     3001 cgatagcgct cgcgcacggc ggagttcacg ccgtccgcgg ccaggatcag atcggcgcct
     3061 tccagcgatg cggggtccgc cacctcgcgg tcgaagcaga ggcgcacgcc cgcctcgcgg
     3121 catcgcccgg ccaggatgcc gagcagcccc gcccgggaga gccccgagaa gcgatgcccc
     3181 ttcgagacga gggtgtgccc cttgtagtga acttcgatgt cgtcccagcg gtggcagcgc
     3241 gcggacatcg cggccgctac gtccgcgtcg gcaacggcga gcgcctcctc ggtcgcgtcg
     3301 gagaagacca cgccgaatcc gaaggtgtcg tcggggcggt tccgctcgta gacggcgatc
     3361 tcgtggttgg gatcggcgcg cttcaagagc agcgcgaaga agagcccggc ggggccgccg
     3421 ccgatgacgg cggccttcat cgcccggcct gcagcacctg accggcgatc acgaggtgct
     3481 ggatctccgt cgtcccttcg tagatgcgga gcgccctgac ggcgcggtac aggcggtcca
     3541 ccggatggtc cgccatcacg cccctggcgc ccaggacctg cacggcgtcg tccacgatcc
     3601 gctgcgcggc ctcggtggcg aacgacttcg ccatcgccgc ctccatcgtg acgcgctcgg
     3661 cgccgtggtc ggcttcccac gcggcgcggt agaccaggag ccgcgcggca tcaagctccg
     3721 tcgccatgcg cgcgatcttc tcctggacca gctggaactc ggcgagcggc tttccgaact
     3781 gcgtccgggc gcgcgcgtgc gccgcggctt cctgcatcgc cctcgcggcc atgccgcagg
     3841 cggccgcgcc gaccgtggcg cgcatccggt ccagcgtctt cagacccagg gcgaagccgc
     3901 gtccctcctc gccgaggagg ttcgccgcgg gaatccggca cccctcgaac atcagctcgc
     3961 ccaaaggatg cggcgccgag agcacctggg ctcccgcgaa gcggagtccc ggcgcgccgg
     4021 ccggcacgag gaacgcggag atccccttgc cgcgcgcgtc cggatcggtc gaggcgaaga
     4081 ccacgtagaa gtcggcgatg ccggcgttcg agatgtacga cttggcgccg tcgaggacgt
     4141 agtcgccgcc gtctttgcgc gcgcgcgttc ggagggaggc cacgtccgat cccgcctccg
     4201 tctcggtcat cgcgaacgag ctcatggcgc gtccggcgat cgcctcctcg gcgaggcggg
     4261 cgcccgcggc ggagccggtg atcaggagcg gcagcgtgcc cagcccctgc agcgcgaaga
     4321 ccgcgtcggc gagcgggctg gccgcggcca gcgcctcacg cgcgaggcag caggcgcgcc
     4381 agtcgccgcg gcggatcggc tccagcatcc ttcccgcccc cagcgccgcc aggatcgcgc
     4441 gcgcctcggt gcgcgccgcc gcgtcggtcg cggcgggggt ccgcagggcg atctcgcgcg
     4501 cggcgaacgc cgcggcttcg gccgcgaacg tttcgtgcga gggatcgaga agggcgcgga
     4561 tgggtcgcgg gtcgggaatc aacggaaccg cggctcccgc ttctcgcgga acgcctcata
     4621 ggcttcgcgg aagttggggt gctccatcag cgcggcctgg gcgtccgact cgtagttcag
     4681 cgcggacacc aggtcctggc tgaactcgta gtcgagcgcc tccttggtca cggcgagcgc
     4741 tgccgagggt ccccgcgcga gccgctcggc gtaggccgtc gcctccgcca ggacgcggtc
     4801 cggcgccgtc acgcgatggt agagcccgat ctcgtaggcg cgcttggcgt cgatgaaatc
     4861 gccggtcatc aggagctcgg tcgcgcgccc cagccccacg atgcgcggca ggagccacga
     4921 ggcgcccatg tcggctccgg agaggccgac ccggacgaag aggaaggcga tcttggcggt
     4981 ttccgaagcg atccggaggt cgcacgccgt ggcgatcacc gcgccggccc cggccgtcgt
     5041 gccgttcagc gcggcgatca ccggcttccg gcatccacgg atgttccgaa tcaggtcgca
     5101 ggtcatgcgc gtgaactcgc ggagcccgcc gaagtcgcgc tcgaacagcg cgccgatgat
     5161 ctcctccacg tcgccgccgg agcagaaacc gcgccccgcg ccggtcagga cgatcgcccg
     5221 cacaccctcc tcctgatcga gcgcggcgaa gaggtcgcgc agctccgcgt agacctcgaa
     5281 cgtgagcgcg ttcaggcgct ccggccggtt cagcgtgatc gtggctacgt tcgtcgcctc
     5341 gtccacggcg tagagaaggg acttcggctg gagcggggta aggtcggccg gtctcggctc
     5401 ctggctcacg cgccacctcc ggagggtccg gcgatcaccg ccgtcgcttc catctccacc
     5461 atcgctcctc ggtccatcag ccccttcacc tccaagagcg ccacggccgg gtagaagcgg
     5521 ccgagccgcg cgcgccacgc ttcgccgagg gcccgccgtg ccgcgcgata ggcggcgagg
     5581 tcggtcacgt agatcgtgag acgggcgaga tcctggggac gtccgccggc ctcggcgatc
     5641 acggagagca ccttgtccag ggcccgcacg aactgcgcgg cgaagtcggg gacgtcgccg
     5701 gaggcctcgt cttcccatcc ggcctgtccc gcgacgaaca aaagccttcc tcccgcgggg
     5761 gcgaggaggc cgttgctcca gcccttcggc tcgcccagcg agcgggggtt cacgatctcg
     5821 aacgtcacgc cgcgtgcacc cgcgcgccca gcacgagcgc ctgtcccgtg gggtgctgcg
     5881 gatcgacgat cagcttcagg acggcccgcg ccacttcttc gggggtgagc agtcgctcct
     5941 ggccggtcgt tccgaggatg gcggccagcg cctcgggatg cgagagcccg ccgcgcgaca
     6001 tcgcgccctc gaccgccttg tgggtgatcg acgtgtccac gtatcccgga cagacggcgt
     6061 tcacggtgac gcccttcccc tccagctcgg ccgccaggga gcgcaccagt cccacgaccg
     6121 cgtgtttcga ggccgcgtag ggtgcgatgt acttccctcc cgtgagtccg gcgaccgaag
     6181 cgacggccac gacgcggccg aagccgtgcg ccgccatggt cggaacgaag gcactcgcgc
     6241 agaggaacgt gctccgggca ttggcgttca tcatcgcgtc ccaatcggag atcgtgacct
     6301 cgcggaaggg agcggccatg gcggcccccg cgctcatcac gagcacctcc ggattaccga
     6361 gctcgatcct cacacgggtc gacagctcgc gcacctcttc ctcgttcgtg acgtcacagg
     6421 agtaaccgga cgcggttcca ccctcgctcc ggatctcgct cgcgaccgca tcgcactcgg
     6481 cgcgggaccg cgcggccacc ccgaccgcgg ctccggactt cgcgaggagc cgggcgatgg
     6541 cggcgccgat gccccgcccg ccgccggtca cgagcgcgac gcgaccatcg agcggccggt
     6601 cagccatggt gccgctccag caccgcctcg gccaggatgg ctccgagcgc ctcgatggtg
     6661 cgcatcccct cttcggcggt ggcgtcggcg ggccagccga agtaggcccg cggtcctccg
     6721 gcgtcctcga acgtgcgcgc gccggcacga atcgcgcgcg acaacgagga aggattgggc
     6781 gcgagggccc ggcgcgtctc ctcgcgcacg aggtccggac ggaccgcgag cacgaccgag
     6841 ccctcgtagc ggcccgcgtg gcaggcgccc gtctggaatt cctcccccag gcggagcgcc
     6901 cagggcttgc gcgtcagatc ggggcagatc acgggaagaa gctcctcttc gtcggccgcg
     6961 gcgcgcgccg cggacagcgt ctcgagatgg gcggggtcga ggtgggcgtt cgccaccccg
     7021 agcgccgcga agccctggcg ggtgagctcc cgcgccaggt cgagcaggac cgctttcacg
     7081 ctcgcggcgc cgaccgagat cgtccccgga aacccggcgc cgaacggggc cgcggcatag
     7141 ggaagcggcg ggagcaccac cgcgatccgt ccctccgcct ccaggcgcgc cgcggcggcg
     7201 acggccatcg cctcggccag gatgacgtcg gtggcgagcg gaaggtgggg cccgtgcgcc
     7261 tcgatggcgc cgaccggaag cagggcgacg gcccggtggc ggtcgagatc ccgcacctct
     7321 tcccaggtca ggtcggccca cttgcgcggc gccacaggtt ccctccgagt cgggtcttgc
     7381 cgcccgcagg ccgggcgtcc atcatgggac cagccacgaa acttaccatg acctacgagc
     7441 cgccggaacg cctgaacctc gccgatcacc tcctcgacgc ccgcgtccgc gaagggcgcg
     7501 gcggccggac ggcgctcgtc accgaccagg gctcgctcac ctacgccgac gtccaagcgc
     7561 tcgcgaaccg cttcggccac ctgatccggg aggccggcgc ggagccggag cagcgggtgc
     7621 tgatcgccct ccccgacggc cccgagttcg tggcggcgct cttcgggacg ctcaagatcg
     7681 gggcggtcgc ggtcatggtg aacccggggc tctccctgga ggagatccgc caccttctgg
     7741 cctatacgcg ggcgcgggtc gccgtcgcgc accgggacac ggcggccgcg ttcgagcggg
     7801 ccgcgggcga ggctcccctc gtgaagcggc tcctgaccgc gggcggcgac cccttcgccc
     7861 gggcgctggc cgccgccccc gccgcgctcg aggccttccc gagccaccgc gacgacccgg
     7921 cgatctggct cttctccggc gggacgacgg ggcggcccaa ggcggtcgtc cagacgcacc
     7981 gctccttcgc ctacaccacg gcgtgctacg gacccgaggt gatcggctac accgagaacg
     8041 acgtgacgct ctccgtgccg aagctctact tcggctacgc cacgggggcc aacctcttct
     8101 ttccgttcgc cgcgggcgga gcgtccgccc tcttccccga gccggcgacc gcggagacgg
     8161 tgttcgcgaa gatccggaag ttccgcccca ccgtgctcgt gaacgtcccg accatggtgc
     8221 acaagatggt gacccacccc ggcgcttcgg ccgaggatct cgcctcgctt cgcgtggcca
     8281 tctccgcggg cgaggcgctt cccgccgagc tctacgagcg ctggaagaag acgacgggcg
     8341 tggagctgtt ggacgggctg ggcaccgccg agatgtggca catcttcatc tcgaaccgga
     8401 tcggcgcggt gcgcccgggg acgctcggca cggtggtccc cggtttcgag atccaggtgt
     8461 gcgacgagga ggggcgcgag ctcccgccgc gcgagacggg atggctccgg gtgcgcggcg
     8521 gcgcacgagc gatcggctac tggcagcatc aagaaaagac cgagcaggcg tttcgcggcg
     8581 agtggtacgt gaccggggac ctggtgcagc gggacaccga cggcacggtc acctactgcg
     8641 ggcgcggcga cgagttgctc aaggtctccg gaaagtggct cgccccggcc gaagtggagg
     8701 ggtgcctgct cgatcatccg gcggtggccg aggccgccgt ggtgggcgcg gccgacggga
     8761 gcggcctggt gaagccgatc gcctacgtcg tggcgcgcga gcgtcgcgag ggcctggccg
     8821 acgagctgcg cgcccacgtg cgcgagcggc tcgcgcccta caagcatccg cgcgaggtgg
     8881 tcttcctgga cgcgctcccc cgaacgcatc tcggcaaggt ggaccggggc cgcctccggg
     8941 cggggtagcg gacgtgactc cggcccgagg cgggccgcgt ggcggccgcg gggcgctcct
     9001 cgtcggcacg tcgggatggg cgtatcgcca ctggccccgc ttctatcctc ccgatctacg
     9061 gccgccccga tacctggcgc actacgccac ccgcttcccg accgtggagg tgaacacctc
     9121 cttctaccac ctgccgctcc gcaccaccta cgcgaagtgg gccgcggaga ccggtcccga
     9181 ttttcgcttc gcgctcaagc tgagccggta cgtcacgcac atcaagcggc tctcgggcgt
     9241 gaaggccgag acgcgcctct tcctcgaacg agccgaaccg ctggggaaga agctgggccc
     9301 catcctggtc cagcttccgc cgcagctccg ggcggatccc atccggctgg agcgcttcct
     9361 cgcgatggtc cgctcgctcg agcaggagcg cggtgagcgc gttcggctgg cgttcgagtt
     9421 ccggcacgag acctggttcg cctccaagga agcgctcgac gcgctcgcgc ggcatggcgc
     9481 ggcgctggtg ttcgggcagt cgggccgcta tccctatccc tccgcggagc cgctcacggc
     9541 gtcgtgggtc tatctgcggt tccacggccc gcgcgagttc tgctcctcgc aatacgggcg
     9601 ggaggggctt gcgccgtggg gggagaagat cgcgcgctgg cgcgccgagg ggcgcgacgt
     9661 ctacgcctac ttcaacaacg acgtgcacgg gtacgcggtg gaagacgcgg cgcggttggt
     9721 tcggatggcg acagcggacc aggcgtgaga agcgcgagga cccggaaatg aaacgcgcgc
     9781 ccgcgacgtt ccgcgggcgc gcctggaatc caatctcccg cggttacgag cgggcgctcc
     9841 tcgaggaaga gcgcgcccgc gacgacgagc tccgcgaggt ggagcggcgg gaggaagagc
     9901 gcgtcgagcg ccccgacgtg gagcgccgcg acctggtgct cgaacgcttc ttgggcgacg
     9961 gcaccttggc gcccttcgcg cgcgcctcgg acaatccgat cgcgatcgcc tgcttccggc
    10021 tggtcacctt ccctccatgc cccgacttcc cactcttcag ggtgcccttc ttgcggcggc
    10081 gcatcgcgct ctcgacggac ttggaagcgg actcgccgta gcggcggccg ctcgattttc
    10141 tcttacgtgt cggcatcggt cttctcctgt tcgatgggcg cgaaccgcgt tcgcgcttcc
    10201 aggcccacga cccctcgtgc ctcgttctgc agtgcacatc ccatgccgac gcgtgcctcg
    10261 ccagctcgcg cgaagcgcct tgaccccctc cgatgatgcg ggtacggtcg cgggaactgg
    10321 atcgtttccg cgcgccaacc catggagccg acatgccccg ccgcgccgcg tcagcccgca
    10381 ggagcccgtc cgcgccgcgg cgctcgccct cgcgccagaa acaggccccg cgcgcgaacc
    10441 gctcggtacg ctccgcaacg tccgccgcct cgaggctcct ggacggctgc ttcaacgtcg
    10501 ccgacctgga ggtcatcgcg aagcggaagc tgccccgccc gacctacgac tactacgccg
    10561 gaggcgccga ggacgagcgg accctggcgc gcaaccgggc ggggttcgag cgctacgccc
    10621 tgctccaccg cgtgctcgtg gacgtcgaac gggtggacac cagcaccacc gtgctcggcg
    10681 ccgcagtcac gaacccggtg ctcgtcgcgc cgaccgccta ccagcgcctc gccgacgacc
    10741 agggggaaac cgccacggcc cgcgcggccg gccaggcggg atcgctcatg acggtgagca
    10801 cgctcgccac ccggtcgctg gaggaggtgg cggcggcggc cacggggccc ctctggttcc
    10861 agctctacgt ctatcgcgac cgcgcggtca ccgagcggct gatcgcccgc gccgaggcgg
    10921 cgggctaccg cgcgctcgtc gtcacggtgg acacgccccg cctgggacgc cgcgagcgcg
    10981 accatcgcaa cggcttcacg ctcccccgcg gcctgtcgat cgcgaacttc acggaggacg
    11041 ccgatcgcgc ggggttggag cggtacggta gcctcgccgc ctacgcctcg gcccagctc
//
LOCUS       DASSLZ010000338         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_878719, whole genome shotgun sequence.
ACCESSION   DASSLZ010000338 DASSLZ010000000
VERSION     DASSLZ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878719"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1058)
                     /locus_tag="VFM00_12710"
     CDS             complement(132..1058)
                     /locus_tag="VFM00_12710"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7499153.1"
                     /translation="MNFQRHSSARLNGRIAALFALFGLLLPPAPAHSAAPPKSQADMK
                     PVVRKAPPPMTVAEAEALVQKSPKDTNAYLALGGAYRRSRRYPQALEAFQKMAALDPQ
                     NSTARYSLGAVYMDMNRPKDAEREIRKAIALSPKDPGAHLQLGLWYVSQGRNDDAVAE
                     FKIATTLPPPLPEAWNKLGRVQAELGQTADAVASYKKALDIDSTNVGALNNLGNSYYG
                     MGKIPEATALYRKALDFDPKNQEAIYNLGVAFADAQIYKEALNYWRQVAAIDSSTAVA
                     QNAKSSIEVLQQYLDQQKATAAPSQAPAPAAH"
     gene            1290..1688
                     /locus_tag="VFM00_12715"
     CDS             1290..1688
                     /locus_tag="VFM00_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499154.1"
                     /translation="MTPKSLQSILVFNSSEETLEILKAALEGTYAVSTGHVADLKRGQ
                     VDLLKLVEEHRPDAILFDIAIPYEENWRFLRLLQSSEALKSVKWVITTTHKKLLEELV
                     GECGEVYEVVGKPYDLAQIMNALKDALGSD"
     gene            complement(1708..2694)
                     /locus_tag="VFM00_12720"
     CDS             complement(1708..2694)
                     /locus_tag="VFM00_12720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HET7499155.1"
                     /translation="MNGSSDEQELFRWWRVFFDASPDAFLLIDQARLVLQANPSARRL
                     LGREPSEVLGRSLDAILVRSPGLAVTYGPPGSEGRHTVVRLERRIADGASSGGMTSLS
                     LAQEYRYILSHELRTPLQAILGWTQILRATPHEPPAIQSALAAIERNVHWQSQVIEDL
                     VQMAELAGEAGALNPRRTSAASIVERAVKWSAPAATSKGVRLAVKRDTPDDEIVADPS
                     LLARALERLVTNAIRFASIGSEVVIRVHQVGSGVRIAVTDDGGQDDPASSHLYGWFRR
                     SNESVSDFVRLGAGLTNVQRVVEMHGGSVAAESEGAGRGATFHVTIPLASDR"
     gene            complement(2922..>4269)
                     /locus_tag="VFM00_12725"
     CDS             complement(2922..>4269)
                     /locus_tag="VFM00_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET7499156.1"
                     /translation="FPARKITINLAPAHVRKEGARFDLPIAVALLRASGQIPGGAPVD
                     EGLFVGELALDGGVRGVRGVLAVLAAARREGRSPVWIPAENAPEARAYPVVATAAIES
                     LGDLRGDEGPPRAAGAPAGPEPGLETQASEDLPDLAEVRGQLHARRALEVAAAGGHNL
                     LFVGPPGGGKTMLARRLPGILPPLRDDEAIEVSTIHSVAGRLAPGAGILRHPPFRAPH
                     HTISDAGLVGGGRGPFPGEASLAHHGVLFLDELPEFRRNAIEALRQPIEEGRITIARA
                     GSSVTFPAAFALVAAMNPCPCGFRGDLRRACRCSSDLVMRYWSKVSGPILDRIDLVLE
                     VPAVPMEDLFASVAAEASEAVRLRVLRARAVAAARAPRARRNAALTAREVARVAPLMP
                     DSRQLLLKAADTFRITARGVLRTRRLARTIADLAGSEEVRGEHVAEALQYRLPTPF"
BASE COUNT          679 a         1428 c         1492 g          670 t
ORIGIN      
        1 atgcgccgag cgccagcgcg agaatcgcgg gaccgacgag atgcagggaa ggtcgttggg
       61 gacgcgtcga gcgggccggc atgacggtga cctcctggat cgtgtcgggc ccgtccacgg
      121 acccgagagg gtcagtgcgc cgcgggagcc ggcgcctgcg agggcgccgc cgtggccttc
      181 tgctgatcga ggtactgctg gaggacctcg atgctgctct tcgcgttctg ggccaccgcc
      241 gtcgacgaat cgatcgcagc gacctgccgc cagtagttga gcgcttcctt gtagatctgc
      301 gcgtcggcga acgcgacccc caggttgtag atcgcttcct ggttcttcgg gtcgaaatcg
      361 agagctttgc gatagagcgc cgtggcttcg ggaatcttcc ccatgccgta gtagctgttg
      421 ccgagattgt tgagcgcccc gacgttggtc gagtcgatgt cgagcgcctt cttgtacgag
      481 gctaccgcgt cagcggtctg tcccagctcg gcctgaaccc ggcccagctt gttccaggcc
      541 tcggggagcg gcggcgggag cgtggtcgcg atcttgaact cggcgacggc atcgtcgttc
      601 cgcccctggc tgacgtacca gagcccgagt tgcaggtggg cgccggggtc tttggggctc
      661 agggcgattg ctttccggat ctcccgctcg gcgtccttgg ggcggttcat gtccatgtag
      721 accgccccca ggctgtagcg agccgtggag ttctgggggt cgagcgccgc catcttctgg
      781 aaggcctcga gcgcctgggg gtagcggcgg gaccgccgat aggcccctcc cagcgcgaga
      841 taggcgttgg tgtccttggg gctcttctgg accagggcct cggcctccgc aaccgtcatg
      901 gggggcggag ccttgcgcac gaccggcttc atgtcggcct ggctcttggg cggcgcggcc
      961 gaatgggccg gggccggggg aagcaggagc ccgaagaggg cgaaaagggc tgcgatacgc
     1021 ccgttcaggc gggcggacga gtggcgttgg aagttcatgg actctttgtc tcgcattggg
     1081 ttaggagcaa cggacacggt accttgaaga taccccgaag tcaacggggt tctcccctat
     1141 gacgggccgg gcgggcgctc gattgcatcg gcgtcgcgcg accggtacgc tgcccccgat
     1201 tggtcccatg cgttcccgaa gttcctgaat cccgcggctg gtattgccgc ttcggccgcg
     1261 gaatgccgcg gttggagcag gaggcgtcga tgactcccaa gagccttcag tcgatcctgg
     1321 tcttcaactc cagcgaggag accctcgaga tcctgaaggc ggcgctcgaa ggaacgtatg
     1381 cggtttcgac gggccatgtc gcggacttga agcggggtca ggtggattta ctgaagctgg
     1441 tcgaggagca ccgtcccgac gccatcctct tcgacatcgc cattccctac gaggagaact
     1501 ggcgcttcct ccggctgctc caatcctccg aagccttgaa gtcagtcaaa tgggtgatca
     1561 cgacgacgca caagaagctc ctcgaagagc tcgtggggga gtgcggcgag gtgtacgagg
     1621 tggtcgggaa gccctatgac ctcgcccaga tcatgaacgc cctgaaagac gcgctgggat
     1681 cggactgacg agcaccgagc ttcgctctca acggtcggac gcaagcggga tcgtcacatg
     1741 gaaggtggcg ccgcgacccg ccccctcact ctccgccgcg acgctgccgc cgtgcatttc
     1801 caccacgcgc tggacgttcg tcaagcccgc ccccagccga acgaagtcgc tgaccgattc
     1861 gttcgacctt cgaaaccagc catagagatg ggatgaggcg gggtcgtcct gacccccgtc
     1921 gtcggtgacg gcgatacgca ccccggaccc gacctgatgc acgcgaatga cgacctcgct
     1981 gccgatcgac gcgaaccgga tcgcgttcgt cacgagccgt tcgagggccc tggcgaggag
     2041 cgatggatcc gccacaatct cgtcgtctgg ggtgtcccgc ttcaccgcca gccgtacccc
     2101 tttcgaggtc gccgcgggag cgctccattt cacggcgcgc tccacgatcg acgcggcgga
     2161 cgtccttcgc gggttgagcg cgccggcctc gcccgccagc tcagccatct gaaccaggtc
     2221 ttcgatcacc tgggattgcc agtgcacgtt tcgctcgatc gccgcgagcg ccgactgaat
     2281 cgccggaggt tcgtgcggtg tcgcgcggag aatctgggtc cagccgagga tggcctgcag
     2341 cggggtgcga agctcgtgcg acaggatgta ccggtattcc tgcgcgaggg aaagcgaggt
     2401 catgccgccg gaggaggccc cgtcggcgat acgacgctcc aaccggacca ccgtgtgacg
     2461 tccctccgag cccggcggac cgtacgtgac cgcgagtccg ggactccgga caaggatcgc
     2521 gtcgagggag cgccctagaa cctcggaggg ctcgcgtccg agaagacgcc tggccgaggg
     2581 attggcctga aggacgagcc gggcctgatc gatcaggagg aacgcgtcgg gagacgcgtc
     2641 gaagaagacg cgccaccaac ggaaaagctc ttgctcgtcc gagctgccgt tcatgggagc
     2701 ctcgttcctg tggtgatcgt ggcggacgaa gcaagcttcg agccaccggc aacccggggc
     2761 ctattcttcg ccaagtccat tacatcctgg gcggttaagc gcggcaagaa cgtcacggcg
     2821 tctcctagcc catggtaccg gcggtaggaa tttcagcgga catcgcctag atgtccgcgc
     2881 cgatccgcct ggtcgaggcc cgtgctcggc cggctacctc gtcagaatgg cgtcgggagc
     2941 ctgtattgga gcgcctcggc gacatgctct ccccggacct cctccgagcc ggccagatcc
     3001 gcgatcgtgc gcgcgagccg gcgcgtgcgg agcacgccgc gcgcggtgat gcgaaaggtg
     3061 tcggcagcct tcaggaggag ctggcgcgaa tcgggcatga gcggcgccac gcgggcaacc
     3121 tcccgcgccg tgagcgcagc gtttcgtctc gcgcgcgggg cgcgcgcggc cgccacggcg
     3181 cgagcgcgga ggacccgcaa gcgaaccgcc tccgaagcct ccgccgcgac gctcgcgaag
     3241 aggtcctcca tcggcaccgc gggaacctcg agcacgagat cgatgcggtc caggatcggc
     3301 cccgacactt tcgaccagta gcgcatgacg agatcggacg agcagcggca cgcgcggcga
     3361 aggtcgccgc ggaagccgca gggacagggg ttcatcgcgg cgaccagcgc gaaggcggcg
     3421 ggaaaggtca ccgacgatcc cgcgcgcgcg atcgtgatcc tcccctcctc gatcggttgc
     3481 cgcagcgcct cgatcgcgtt ccgccggaac tcgggcagct cgtcgaggaa aaggacgccg
     3541 tgatgcgcga gggaggcctc gccgggaaaa gggccgcgtc cgccgccgac gagcccggcg
     3601 tcggagatcg tgtgatgggg cgcgcggaag ggcggatggc ggaggattcc cgcgcccggc
     3661 gcgagccggc ccgccaccga gtggatcgtc gagacctcga tcgcctcgtc gtcccgtagc
     3721 ggcgggagga tgccggggag ccgccgcgcg agcatcgtct ttccgcctcc cggcgggccg
     3781 acgaagagaa ggttgtgccc gccggccgcc gcgacctcca gcgcgcgccg cgcatggagc
     3841 tgcccgcgca cctcggccag gtcgggcagg tcctcggatg cctgcgtctc gagaccgggc
     3901 tcgggcccgg cgggagcccc agccgcgcgc ggcggccctt catcgccgcg cagatcgccc
     3961 agagactcga tcgccgccgt ggccacgacg gggtacgcgc gtgcctccgg cgcgttctcc
     4021 gccggaatcc agaccggcga tcgcccctcg cgccgcgccg ccgcgagcac cgcgagaacg
     4081 ccgcgcactc cccgcacgcc gccgtcgagc gcgagctctc ctacgaacag cccctcgtcg
     4141 acgggcgccc ctcccgggat ctgccccgag gcgcggagca gcgccacggc gatcggaaga
     4201 tcgaaccgcg cgccctcctt tcgcacgtgc gccggcgcca gattgatggt gatcttccgc
     4261 gccgggaac
//
LOCUS       DASSLZ010000339         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1746721, whole genome shotgun sequence.
ACCESSION   DASSLZ010000339 DASSLZ010000000
VERSION     DASSLZ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746721"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFM00_12730"
     CDS             <1..588
                     /locus_tag="VFM00_12730"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET7499157.1"
                     /translation="VRRSDPDYDALGLMNTILGGGFTSRINQNLREKNGYTYGTYSAV
                     SENAGPGRLVVAGGIRTDVTGPAITEIFKEVRGMKEGAVTEAELARARGARIQALPGR
                     FETSGYTAGQIGNLFTFGLPDDYFQTLPARLGKITAEDLSAMARKYLTPDQMLIVAVG
                     DRAKIAPQIQPLGLGPVALSDADGKALDAESAVTK"
     gene            594..1088
                     /locus_tag="VFM00_12735"
     CDS             594..1088
                     /locus_tag="VFM00_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HET7499158.1"
                     /translation="MSPKGAGGRATTAALSLLAWLTITFAAALVGSRFRPGPWYASLA
                     KPDWTPPGAVFAPVWTALYLAMGIAAWMVWRRGGGGRSRAPALALYMVQLALNALWSF
                     LFFGLHRPGLAFLDIALLWAAILTVMVLFWRIRAAAGALILPYLLWVSFAACLNFAIW
                     RLNP"
     gene            1085..>2128
                     /locus_tag="VFM00_12740"
     CDS             1085..>2128
                     /locus_tag="VFM00_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET7499159.1"
                     /translation="MTPGVVLLTGASGYVGSRLRAALEARGVRLRCMAREPRLLAPHV
                     AAATEVVRGDALDPASLGPALQNVATAYYLIHSMASGRAFEEHDRAAARNFGAAARAA
                     GVRRIIYLGGLGHGPGLSPHLASRQETGRLLRESGVPVIELRASVIIGSGSASFELVR
                     GLVDRLPVMTTPRWVSTEAQPIAIEDVIEYLVLALDLPEEESRIYEIGGADRSSYRDL
                     MREYADRRGLKRWLIRLPFLTPRLSSLWLRFVTPLYASVGRELIEGVRNPSVVEDPSA
                     LRDFPLQPRGMREAITRALANEDAEMAATRWNDVRAVPPSWGGVRFGSRLVDSRSLRV
                     DASPAAAFAAIRSI"
BASE COUNT          282 a          753 c          751 g          342 t
ORIGIN      
        1 gtccgccgca gcgatccgga ctacgacgcg ctcggcctca tgaatacgat tctcggtggc
       61 ggcttcacca gccggatcaa ccagaacctg cgcgagaaga acggctacac gtacggaacg
      121 tactcggcgg tctccgagaa cgcgggaccc ggccggctcg tcgtcgccgg cggaatccgc
      181 accgacgtga ccggtccggc gatcaccgag atcttcaagg aagtgcgcgg catgaaggaa
      241 ggagcggtca ccgaggccga gctggctcgc gcgcgcggcg cccggatcca ggccctgccc
      301 ggccgcttcg agacctcggg ctacacggcc gggcagatcg ggaacctgtt cacgttcggc
      361 ctgcccgacg actacttcca gacgctgccc gctcgtctgg gcaagatcac cgcggaggat
      421 ctctccgcga tggcgcggaa atacctcaca cccgaccaga tgctgatcgt ggcggtggga
      481 gaccgcgcca agatcgcgcc gcagatccag ccgctgggac tggggccggt cgcgctcagt
      541 gacgccgatg gaaaggcgct ggacgccgaa tcggccgtga cgaagtagca cccatgagcc
      601 cgaagggcgc cggcggacgc gccacgaccg ccgccctttc gttgcttgcg tggctcacga
      661 tcacgttcgc ggccgcgctc gtgggctccc gatttcggcc gggcccctgg tacgcgtcgc
      721 tcgccaagcc ggattggacg ccgccgggcg cggtgtttgc gccggtctgg acggcgctct
      781 acctggcgat gggaatcgcc gcgtggatgg tgtggcggcg cgggggcggg ggccggtccc
      841 gggctcccgc gctcgcgctc tacatggttc agctcgcgct caacgcgctc tggtcgtttc
      901 tcttcttcgg gctgcaccgc cccggcctcg ccttcctcga catcgccctg ctctgggccg
      961 ccatcctgac ggtgatggtt ctcttctgga gaatcagggc cgccgccggc gcgctgatcc
     1021 ttccctacct cctgtgggtc tcgttcgccg cctgcctgaa tttcgcgatc tggcgcctga
     1081 atccgtgacg ccgggggtcg tcctgctcac gggagcctcg ggatacgtgg ggagccggct
     1141 gcgcgccgcc ctcgaggcgc gcggggtgcg gctccgctgc atggcgcgcg agccccggct
     1201 cctggcgccg cacgtcgcgg cggccaccga ggtcgtgcgg ggcgacgcgc tcgaccccgc
     1261 gagcctcggg cctgccctgc agaacgtggc caccgcgtac tacctgatcc actccatggc
     1321 ttcagggcgc gcgttcgagg agcacgaccg cgcggcggca cgaaacttcg gagccgccgc
     1381 ccgcgcggcc ggagtgcggc ggatcatcta cctgggcggc ctgggtcatg ggcccggcct
     1441 ctcgccccac ctcgcgagcc gccaggaaac cggccgactg ctccgggagt cgggcgttcc
     1501 ggtgatcgag cttcgcgcgt cggtgatcat cggctcgggg agcgcctcct tcgagctggt
     1561 ccgcgggctc gtcgaccgcc tgccggtgat gacgacgccg cggtgggtga gcaccgaggc
     1621 ccagccgatc gcgatcgagg acgtgatcga atacctggtg cttgcgctgg acctccccga
     1681 agaggagagc cggatctacg agatcggggg cgcggaccgt tcctcctacc gcgacctgat
     1741 gcgggaatat gccgaccggc gcggcctcaa gcgatggttg atccgccttc ccttcctgac
     1801 cccgcggctc tcctcgctat ggctgcggtt cgtgacgccc ctctatgcga gcgtcgggcg
     1861 cgagctgatc gagggcgttc gcaatccgag cgtggtggag gatccgtcgg cgcttcgtga
     1921 ttttccgctc cagccgcgcg ggatgcgcga ggcgatcacc cgcgcgctcg cgaacgaaga
     1981 cgcggagatg gccgcgacgc gctggaacga cgtccgtgcc gtgccgccgt catggggcgg
     2041 cgtccggttc gggtcgcgcc tcgtggactc gcgctcgctg cgcgtggacg cgtcgccggc
     2101 cgccgcgttc gcggcgatcc ggagcatc
//
LOCUS       DASSLZ010000340         4533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1747460, whole genome shotgun sequence.
ACCESSION   DASSLZ010000340 DASSLZ010000000
VERSION     DASSLZ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747460"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2380
                     /gene="polA"
                     /locus_tag="VFM00_12745"
     CDS             <1..2380
                     /gene="polA"
                     /locus_tag="VFM00_12745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET7499160.1"
                     /translation="LDALYASLDQVERPSIREKLRQNEDRARLSYKLATIRSDLPLER
                     HWHELERAPVEYVPLLALLEDLELRVLARRYREEYASRGGAPLAPAPASEAPPPEEIA
                     KVAGVAPPPSDAAASNGTSEAAAPPQPVSPAPAPSSEAPLEGARPARARRGGKAAKSA
                     VPAAAVGQPVGTIFDVLGEGESPMRDAPSGRAPLGRYHVLERGADLDALARELADARG
                     PVAFDTETTGFDPLRSHPVGIALATAEGRAFYLPLGHASGRNLDPDRAREAIAPFFAR
                     RDAVRAAQNAKYDWHVLDRFGVPVRDVAFDTMVAAFLIDPDQPKSLDHLAKARLGIAK
                     IPTEALLGKGREQVTMASVSVDRVAEYCCEDADVTLRLVPLLQRELDSLELTPLFRDV
                     EMPLVGVLTRMERAGVRVDTEALEALGRGLQSQLDDLAHNIEETAGVPFNVNSPRQVA
                     EVLFERLGLPKGKRTREGYSTDTEVLERLALLHPLPKLLLEWRQSSKLKSSYVDSLPR
                     LVHPETGRVHATFHQTIASTGRLSVSDPSLQNIPIRTEEGREIRRAFVAEGPGGLLAS
                     FDYSQIELRILAHLTHDPALTEAFRKDGDVHASTASRLFGVAPELVTPAQRAQAKVVN
                     FGIMYGMGPVRLARELSLSRALAQAFIDEYKRTHAGVATFMEQTLAQARERGYVETIL
                     KRRRPLPNLCDPHDALRAEAERAAINAPIQGSAADLIKVAMVRIDALLAEKGFRSRLI
                     LQVHDELLFETSEEEIEALTPLVVETMEHALPLRVPLVVHRGVGKSWALAHA"
     gene            2421..3023
                     /gene="coaE"
                     /locus_tag="VFM00_12750"
     CDS             2421..3023
                     /gene="coaE"
                     /locus_tag="VFM00_12750"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET7499161.1"
                     /translation="MLRIGVTGLMASGKSTVARRFEERGAVRIDGDALGWEALRIPAV
                     RDRIAATFGAETIAPDGAVDRARLGAVVFADFAAMAKLDAIVQPPLLAMVRRSLDESV
                     APVVVLDAAMLASWGLDREMDGVVEVKAPEPLRIARLRAARGYGAEEAERRVRGQALP
                     PLHAVKRHWVVVNDADAAALRARADQVWGEIVSLAATPEP"
     gene            3042..4146
                     /gene="prfB"
                     /locus_tag="VFM00_12755"
                     /pseudo
     CDS             3042..4146
                     /gene="prfB"
                     /locus_tag="VFM00_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_152559318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            4143..>4533
                     /locus_tag="VFM00_12760"
     CDS             4143..>4533
                     /locus_tag="VFM00_12760"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HET7499162.1"
                     /translation="MSPLVPTIFREYDIRGIADKELTDDTVRAVGRAFADRMSERGRT
                     RIAVGRDVRLSSPRIAAALTAGLVERGMHVIDVGQVPTPALYFAILEQKAGGGVMVTG
                     SHNPIEYNGLKLSEGLASLHGEEIQALW"
BASE COUNT          687 a         1588 c         1627 g          631 t
ORIGIN      
        1 cctggacgcg ctgtacgctt cgctcgacca ggtggagcgt ccctcgatcc gcgagaagct
       61 tcggcagaac gaggatcgcg cgcgcctctc ctacaagctg gccacgatcc gctccgacct
      121 gcccctcgag cgccactggc atgagctgga gcgcgcgccg gtggagtacg tgccgctgct
      181 cgcgctgctc gaggatctcg aattgcgcgt cctggcccgc cgctatcgcg aggagtatgc
      241 ctcccgcggt ggcgcgcccc tcgctccggc gccggcgagc gaggcgccgc ctcccgaaga
      301 gatcgcgaag gtcgcgggcg tcgcgccgcc gccctccgac gcggccgcgt ccaacgggac
      361 ttcggaagcg gcggcgccgc cgcaaccggt gtcgccggct ccggccccct cgtcggaagc
      421 gccgttggaa ggggcgcggc ccgctcgcgc caggcgcggc gggaaggccg cgaagagcgc
      481 cgtcccggcc gcggccgtgg ggcagccggt gggaaccatc ttcgacgtcc tcggcgaggg
      541 ggaatcgccg atgcgcgacg ctccctcggg cagagcgccg ctcggccgct accacgttct
      601 cgaacgcggc gccgacctcg acgcgctcgc gcgcgagctg gccgacgcgc gggggccggt
      661 cgcgttcgac accgagacga cgggcttcga cccgctccgc tcccaccccg tcggcatcgc
      721 gctggcgacc gccgagggga gggccttcta ccttcccctg gggcacgcct cggggcggaa
      781 cctggatccc gaccgtgccc gcgaggcgat cgcgcccttc ttcgcgcggc gagacgcggt
      841 ccgcgcggcg cagaacgcga agtacgactg gcacgtgctc gaccgattcg gcgttccggt
      901 tcgcgacgtt gccttcgaca cgatggtcgc ggccttcctg atcgaccccg accagccgaa
      961 gtcgctggat cacctggcca aggcgcggct cggcatcgcc aagatcccga ccgaagcgct
     1021 cctcggcaag gggcgcgagc aggtgacgat ggcctcggtg tcggtcgacc gggtcgccga
     1081 atactgctgc gaggacgcgg acgtgacgct gcgcctggtg ccgctcctcc agcgcgagct
     1141 ggacagcctg gagctcacgc ccctcttccg ggacgtcgag atgccgctcg tgggcgtgct
     1201 cacgcggatg gagcgcgccg gcgtgcgggt cgacaccgag gcgctcgagg cgctggggcg
     1261 gggactccag tcgcagctgg acgacctggc gcacaacatc gaggagaccg cgggggtccc
     1321 gttcaacgtg aactcgcccc ggcaggtcgc cgaggtgctc ttcgagcggc tcgggctgcc
     1381 gaaggggaag cgcacgcgcg agggctactc gaccgacacc gaggtgctgg agcggctggc
     1441 gctcctgcac ccgctcccga agctcctgct cgagtggcgg cagtcctcga agctgaagag
     1501 ctcgtacgtg gactccctgc cgcgcctggt ccaccccgag accgggcgcg tgcacgccac
     1561 gttccaccag acgatcgcct cgacggggcg gctctccgtg agcgacccca gtcttcagaa
     1621 catcccgatc cgcaccgagg aggggcgcga gatccgccgc gcgttcgtgg ccgagggccc
     1681 cggcgggctc ctggcctcat tcgactactc gcagatcgag ctccggatcc tggcccacct
     1741 gacccacgat ccggccctga ccgaggcctt tcgcaaggac ggcgacgtgc acgcctcgac
     1801 cgcctcgcgc ctcttcggcg tcgcccccga gctggtgacg ccggcgcagc gcgcccaggc
     1861 gaaggtcgtg aacttcggca tcatgtacgg catggggccg gtgcggctcg cgcgcgagct
     1921 ctcgctctcg cgcgcgctgg cccaggcgtt catcgacgaa tacaagcgga cccacgccgg
     1981 cgtggcgacc ttcatggagc agaccctggc gcaggcgcgg gagcggggct acgtcgagac
     2041 gatcttgaag cggcgccgcc cgcttccgaa tctctgcgat ccgcacgacg cgctccgggc
     2101 cgaagccgag cgcgcggcca tcaacgcgcc gatccagggc tcggccgccg acctgatcaa
     2161 ggtcgccatg gtccggatcg acgcgctgct cgccgagaag ggattccgct cgcgcctcat
     2221 cctccaggtc cacgacgagc tcctcttcga gaccagcgag gaggagatcg aggcgttgac
     2281 tccgctcgtc gtcgagacga tggagcacgc gctgccgctc cgcgttccgc tggtggtcca
     2341 tcgcggcgtg gggaagtcgt gggccctggc ccacgcctaa gaccttgacc gcgcacgacg
     2401 ccgcccgata gggtcgatcc atgctccgga tcggagtgac cgggctcatg gcgtcgggaa
     2461 aatcgaccgt ggcgcggcgc ttcgaggagc ggggcgccgt ccgcatcgac ggcgacgccc
     2521 ttggctggga ggcgctccgg attcccgcgg tgcgggatcg gatcgccgcg accttcggag
     2581 ccgagacgat cgcgccggac ggggcggtgg atcgagcccg gctcggcgcg gtggtcttcg
     2641 ccgactttgc cgcgatggcg aagctcgacg ccatcgttca gccgccgctt ttggcgatgg
     2701 tccgccgctc gctggacgag tcggtggcgc cggtggtggt gctggacgcg gcgatgctcg
     2761 cgagctgggg gctcgaccgc gagatggacg gcgtcgtgga ggtcaaggcg ccggagccgc
     2821 tgcggatcgc ccgcctccgg gcggcgcgcg gctacggcgc cgaagaggcc gagcggcgcg
     2881 tgcggggaca ggcgctcccg ccgctccatg ccgtgaagcg gcattgggtc gtcgtcaacg
     2941 atgcggatgc cgcggcgctt cgggcgcgcg cggaccaggt gtggggcgag atcgtttcgc
     3001 tcgcggccac gccggaaccc tgaccgggag gatttcgacg gatggacctg aacgcgacga
     3061 agaagcggct cgacgaaggg cggcgcatcc tgacccacct cgggagctat ctttgacgtc
     3121 gcgcagcgcg aggcgcgcgc gggcgagctg gaggcccgca tggccgaatc ggacttctgg
     3181 gagaatccgg aagccgcgca gcggatcctg accgagctca agtcgctcaa gcggatcgtc
     3241 gagccctacc gcgcgctggt ccgcgagatc gacgagacgc agggcatggt cggccttctc
     3301 gaggacgagc cggacgactc gatcgcggcc gagctggaga agtcctcgga gaagctcctt
     3361 ccgcgcatcc agcagctgga gctggtcagc atcctctccg acgagagcga cgctcacggc
     3421 gccatcgtct ccatccaccc cggagcggga ggcaccgagt cgcaggactg ggcgcagatg
     3481 ctcttccgca tgtacgggcg gtacgtcgag cggatgggat ggagctacca ggtgctcgac
     3541 ctccagccgg gggaggaggc ggggatcaag gacgccacga tccaggtgga cggcgcctac
     3601 gcctacggct acctcaaggc ggagcgtggg gtgcaccggc tggtccgcat ctccccgttc
     3661 gacgcgcaga agcgccggca cacgtccttc gcctcggtct tcgtctatcc ccaggtgggc
     3721 gacggcggcg acgtcgagat caaggacggc gacatccgca tcgacacctt ccgggcggga
     3781 ggcgcgggcg gccagcacgt gaacaagacc gaatcggcca tccgcatcac ccacctgcct
     3841 acaggcctcg tggtgcagtg ccagaacgag cgcagccagc accggaaccg cgacgccgcc
     3901 atgagcgtgc tcaaggcgcg gctctacgcg ctccggctgg aggaggagaa ggagaagcgc
     3961 gattccctgg ggggtggcaa gaaggacatc tcctggggca gccagatccg gtcctacgtc
     4021 ttccagccct atacccaggt caaggaccac cgcaccgacg tggtgacgag cgacgtgcag
     4081 ggcgtcatgg acggcgttct cgagccgttc gtggacgcgt accttcgcaa aaaggagacg
     4141 gcgtgagccc cctcgttccg acgattttcc gggagtacga catccgcggc atcgcggaca
     4201 aggagctgac cgacgacacc gtgcgcgccg tggggcgcgc gttcgccgac cgcatgagcg
     4261 agcgcggcag gacgcgcatc gcggtgggcc gcgacgtgcg cctctcctcg ccgcggatcg
     4321 ccgcggccct cacggccggg ctggtcgagc gcggcatgca cgtgatcgac gtggggcagg
     4381 tgcccacgcc cgccctctac ttcgccattc tcgaacagaa agccggcggc ggcgtgatgg
     4441 tcacggggag ccacaatccg atcgagtaca acgggctcaa gctgtccgag ggactggcct
     4501 cgctccatgg cgaggagatc caggccctgt ggc
//
LOCUS       DASSLZ010000341         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1010381, whole genome shotgun sequence.
ACCESSION   DASSLZ010000341 DASSLZ010000000
VERSION     DASSLZ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010381"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1770)
                     /locus_tag="VFM00_12765"
     CDS             complement(202..1770)
                     /locus_tag="VFM00_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HET7499163.1"
                     /translation="MVRTPTRRRGTAGRTALKQEGFATRSRDLPPASPESAHFDASRP
                     GPSDEPLRPAAPAAHRAVTAVAIAADLAAIASAFFLTALVRFTPERLLTPLARPDGLA
                     LFGCSALLWVAFLSAREAYAPRAMISITGQLLRVLGAALPAWVLTHLVAFALKIEVPF
                     ESRLAVGLSLPATLLFLVLGRLLVVRPLARQVYRRLAHGPVLVLGDSDRAVRLVEHYH
                     ESYDGARAVHLHELSSTTPESARDLVETLGVSEVVIEPQGRALEEVFDTAFACLDAAA
                     EVVVVSNQLHVVTGRSSIADLAGVPAMRFRRPDLRGPEAPLRRVIDIAGSAACLVLLA
                     PLFLLIAIAVKFSSPGPIFFRQERVGRRGRRFTMLKFRTMVSGNDPSAHRDYLASYIQ
                     SGAPASVAADGTKIYKLTQDPRVTPVGAGLRAFSLDELPQLWNVLVGEMSLVGPRPCL
                     PYEWDLYRPWQRRRLDVMPGCTGLWQVTARSHVAFDEMVILDLHYAHHGSIGRDLWLI
                     AQTLPAMIRGRGGY"
     gene            complement(1830..2993)
                     /locus_tag="VFM00_12770"
     CDS             complement(1830..2993)
                     /locus_tag="VFM00_12770"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET7499164.1"
                     /translation="MTMVETSPGTGLAPIPVTRLDQADPKLMAELLEVVERVALAGAF
                     TLGSELEAFEAEYAAYCEAPHAVGVSSGTEAIVLALRALGIGPGDEVIVPAHTFIATA
                     EAVSHVGAKPVFVDVRRDTANLDASLLAAAVTPRTKAILPVHLYGQPADMEPILRFAS
                     ERGLIVIEDACQAHGAHYRGRRVGSFGRAGCFSFYPGKNLGAYGEGGAIATNDHELAT
                     RLRRLRDHGQTERYHHAEVGYNARLEGIQGAVLGVKLQHLDRWNRARRAHAAVYEEEL
                     AVAGVAIPFEAESCESVYHLFVIRSKQREALREHLGALGVQTGLHYPIPVHLQDAYAA
                     HGHHEGDFPEAEAWARECLSLPMFAELTFQQMDEVIQGVKSFGLTAAPQANKG"
     gene            complement(2990..>3293)
                     /locus_tag="VFM00_12775"
     CDS             complement(2990..>3293)
                     /locus_tag="VFM00_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7499165.1"
                     /translation="HMRKRRAIPAAEVFGADDRLPEGNEDRARELRSALADLPEEQRD
                     VIVLRHVVGLSPGEIAVRLGKTEPSIHGLHHRGRAALRLALVERGCAPTVASGVAA"
BASE COUNT          522 a         1171 c         1162 g          438 t
ORIGIN      
        1 tccgacgcac cgcaagtgcg gaaatcgctc gcgcgcgcgc tccagattcg acgtgctcgc
       61 gtcgcacacg gtgacgccgt cacacttccg attcgaaagc agattccgaa cgagattcgg
      121 tccccagtac ccgcagccga tcaccccgac gtgcatcatc ccctccccgc ggccccgaga
      181 cccgccgcaa cgatcccgcc cctagtagcc gccccttcct cgaatcatcg cgggcagcgt
      241 ctgagcgatc agccacagat cccggccgat gctcccgtga tgcgcgtagt gcagatccag
      301 aatcaccatc tcgtcgaacg ccacgtggct gcgcgcggtc acctgccaga gcccggtgca
      361 tccgggcatg acgtccaggc gccggcgctg ccagggccgg tagagatccc attcgtacgg
      421 aaggcagggg cgcgggccca ccaggctcat ctcccccacg agcacgttcc agagctgcgg
      481 cagctcgtcg agcgaaaacg cgcggagccc cgcgccgact ggggtgacgc ggggatcctg
      541 cgtgagcttg tagatcttgg ttccgtcggc ggcgaccgac gcgggggcgc cgctctggat
      601 gtaggacgcc aggtaatcgc ggtgggcgct gggatcgttg ccggacacca tcgtgcggaa
      661 cttgagcatg gtgaaccggc ggccgcggcg tccgacccgc tcctggcgga agaagatcgg
      721 cccgggcgag gaaaacttca ccgcgatcgc gatcagcagg aacaaaggcg cgagcaggac
      781 caggcaggcc gccgagccgg cgatgtcgat cacgcgccgc agcggcgcct cggggccgcg
      841 caaatcggga cggcggaagc gcatcgccgg aacgcccgcc aggtcggcga tcgaggagcg
      901 tcccgtcacg acgtggagct gattcgagac cacgaccacc tcggccgcgg catcgaggca
      961 ggcgaaagcg gtgtcgaaca cctcctccag ggcgcggccc tgcggctcga tcaccacttc
     1021 gctcacgccc agggtctcca ccaggtcgcg cgcgctctcg ggcgtcgtcg aggagagctc
     1081 gtggagatgc accgcgcgcg cgccgtcgta gctctcgtga tagtgctcga ccagccgcac
     1141 cgcgcggtcg ctgtcgccca gcaccagcac cggcccgtgg gccagccggc ggtagacctg
     1201 tcgcgccagc ggccgcacga ccaggagccg tcccaggacc aggaagagca gcgtcgccgg
     1261 cagcgatagt cccacggcga gacggctctc gaacggaacc tcgatcttca gcgcgaacgc
     1321 gacgaggtgc gtgagcaccc aggcggggag ggcggcgccg agcacgcgca ggagctgccc
     1381 cgtgatggag atcatcgcgc gcggcgcgta cgcctcgcgc gcgctcagga acgcgaccca
     1441 gagaagcgct gaacatccga agagcgccag gccgtcgggc cgcgcgagcg gcgtcagcag
     1501 gcgttcgggc gtgaagcgga cgagcgcggt gaggaagaac gcggaagcga tcgcggcgag
     1561 gtcggcagcg atcgcgacgg ccgtgacggc gcgatgcgcc gcgggcgccg cgggacgaag
     1621 gggttcgtcc gagggacccg gtcgcgaagc gtcgaaatgt gcgctctcgg gcgaagccgg
     1681 ggggagatca cggctccgcg tggcgaaacc ttcctgcttc agtgcggtcc tcccggcggt
     1741 gcctcgccgc cgtgtcggtg tgcggaccac attcgggggc cttccctgcc ccgccacgaa
     1801 acgcagtaga ccagcgtaga gctaccgagt caacccttat tcgcttgggg cgcagccgtt
     1861 aagccgaacg acttcacgcc ttggatgacc tcgtccatct gctggaaggt gagctcggcg
     1921 aacatcggca aggagagaca ctcgcgcgcc cacgcctcgg cctcgggaaa gtctccttcg
     1981 tggtgaccgt gcgccgcgta ggcgtcctgg aggtgcaccg ggatcggata gtgcagcccg
     2041 gtctgcaccc cgagcgcccc gagatgctcg cgcagcgctt cgcgctgctt cgagcggatg
     2101 acgaagaggt ggtagaccga ttcgcacgat tccgcctcga aggggatcgc gacccccgcc
     2161 accgccagct cctcttcgta gaccgcggcg tgcgcgcggc gcgcgcggtt ccagcggtcc
     2221 aggtgctgca gcttcacccc cagcaccgcg ccctggatcc cctcgaggcg agcgttgtac
     2281 cccacctcgg cgtggtgata gcgctccgtc tggccgtggt cgcgcaggcg gcgcaggcgc
     2341 gtggccagct catggtcgtt ggtcgcgatc gcgcccccct cgccgtaggc ccccaggttc
     2401 tttcccggat agaaggaaaa gcagcccgcg cgtccgaacg atcccacgcg ccggccgcgg
     2461 tagtgcgcgc cgtgcgcctg gcaggcgtct tcgatcacga tcagcccgcg ctcggaggcg
     2521 aagcggagga tcggctccat gtccgcgggc tggccgtaga gatgcaccgg caggatcgcc
     2581 ttcgtgcgcg gcgtgaccgc ggccgcgagc agcgaggcgt cgaggttcgc cgtgtcgcgg
     2641 cgcacgtcca cgaacaccgg cttggcgccc acgtgcgaga ccgcttcggc ggtcgcgatg
     2701 aaggtatggg cggggacgat cacctcgtcc ccgggcccga tgcccagggc gcgcagcgcg
     2761 agcacgatcg cctcggtgcc cgacgacacg ccgacggcgt gcggcgcctc gcaataggcc
     2821 gcgtactcgg cctcgaaggc ctcgagctcg ctgccgagcg tgaacgcccc ggccagcgcg
     2881 acgcgctcga cgacctcgag cagctccgcc atgagcttcg ggtcggcctg gtcgagccgg
     2941 gtgaccggga tcggtgccag gcccgtgccc ggcgacgtct cgaccatggt catgcggcca
     3001 cccccgaggc aacggtcggg gcacagcccc gctcgacgag cgccaggcgg agcgccgccc
     3061 ggccacgatg gtgcagcccg tggatcgagg gctccgtctt gcccagacgc accgcgatct
     3121 ccccggggga gaggccgacg acgtgccgca ggacgatcac gtcgcgctgc tcctcgggca
     3181 ggtcggccag cgcgctgcgc agctcgcggg cgcggtcctc gttgccctcg ggcaggcggt
     3241 cgtcggcccc gaagacctcg gccgccggga tcgcgcggcg cttgcgcatg tgg
//
LOCUS       DASSLZ010000342         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_123665, whole genome shotgun sequence.
ACCESSION   DASSLZ010000342 DASSLZ010000000
VERSION     DASSLZ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123665"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1810)
                     /locus_tag="VFM00_12780"
     CDS             complement(<1..1810)
                     /locus_tag="VFM00_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7499166.1"
                     /translation="MPPNPWRLAFLVPLLALSLVSCSEKQATEPVVNPPGGSPPPGTD
                     TTPPTVTQTQPSNQANGVDRGTAIHAVFSESVAESTATGQTFVVVGDAPVTGTVSATG
                     STVTFTPSSALQGEKTYTATLTTGIYDRAGNHLAQNYTWSFTTAGQAPVANAGPDQAV
                     EAGATVVLNGSGSFDPDQKPLSYTWTQTAGPDVTNGVGYLITASPSFQAPAQATTIEF
                     NLTVSDGIQTSLPDQIRIYVSVTPPVDHTPPSVTSTDPANNATGVDRGTTVRASFSES
                     IDEATATSQTFRLEQNGSAVGGAVSASGSTILFTPSSALQGDRTYTATITTGIHDLAG
                     NAMAQEYTWTFRTAGQPPIANAGPDQSVDAGATVTLDGTGSVDPDGSPLTYTWSQTAG
                     IDVTGGSGTFSGATPSFTAPSSATTLTFSLVVNDGTSSSTPDVVQIVVNAVLTGVFVR
                     PNGDDANAGTKEAPLRTVAAAIAAAPAHGGTVYLAKGTYSQDQFALASGVSLYGGYDD
                     AFQNRDPAANVTVFSGGAIALLADGVSNARVDGITIRSADATGPGSSSYAAFLRGSSG
                     VTFHECTLEAGKGAAGTGGTNGSAGTPGSSGVAGGSG"
     gene            2043..2834
                     /locus_tag="VFM00_12785"
     CDS             2043..2834
                     /locus_tag="VFM00_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7499167.1"
                     /translation="MPPVSKVVVVMPAYNAEATLRKTYEEVMAQGVVDLVIVVDDRSR
                     DATVEVARTLPGVQVVVHERNLGYGGNQKTCYRAALAAGGDIVIMVHPDYQYTPKLIP
                     AMVAMIRSGLYDCVVASRILGGYALKGGMPLWRYVANRGLTLIGNLFMGAKLSEYHSG
                     YRAFSRRVLESLPLDRNSDDFVFDNQMLAQVIWAGFQVAEVSCPTRYAADASSINFAR
                     SIRYGFGCVGTAIEFALARWGVARSTHFPPPLRGRARTQAETLRA"
BASE COUNT          405 a          989 c         1036 g          482 t
ORIGIN      
        1 agccgctgcc cccggcgacg ccgctcgatc caggagtccc ggccgatccg ttcgtgccgc
       61 ccgttccggc cgctcccttg cccgcctcga gcgtgcactc atggaaggtc acgccggaag
      121 acccgcgcag aaacgccgcg tacgagctcg aacccggccc cgtcgcatcg gcgctccgga
      181 tcgtgatccc gtcgacgcgc gcgttgctca cgccatccgc caggagcgcg atcgcgccgc
      241 cggagaacac cgtcacgttg gctgccggat cgcggttctg gaaggcatcg tcgtaaccgc
      301 cgtagagcga gacgcccgac gcgagcgcga actggtcctg ggagtaagtg cccttggcga
      361 gatagaccgt cccgccgtgc gccggcgcgg ccgcgattgc cgccgcgacg gtccgcaggg
      421 gtgcttcctt ggtaccggcg ttggcatcgt caccgttggg ccgcacgaag acgccggtga
      481 gaaccgcgtt gacgacaatc tgaaccacgt ccggcgtgct cgacgacgtg ccgtcgttca
      541 cgacgaggct gaatgtgagc gtggtcgccg aggagggcgc cgtgaagctc ggtgtggccc
      601 cggagaaggt gcccgacccg ccggtcacgt cgattcccgc cgtctggctc cacgtgtagg
      661 tcaaggggct gccatccggg tcgacgctcc ccgttccgtc gagtgtcacg gtcgctccgg
      721 catccacgct ctggtccggc ccggcgttcg cgatcggggg ctgcccggcc gtgcggaagg
      781 tccaggtgta ctcctgcgcc atcgcgttgc cggcgagatc gtggattccg gtcgtgatcg
      841 tggcggtgta ggtgcggtcc ccctgcaacg cgctcgaggg agtgaacagg atcgtggagc
      901 cggacgccga gacggcgccg ccgaccgcgc tcccgttctg ctcgagccgg aaggtctgtg
      961 acgtggcggt tgcctcgtcg atcgattcgg agaaggaggc gcgcacggtg gtgccgcgat
     1021 ccacgcccgt tgcgttgttc gccgggtccg tggaggtgac gctcggcggc gtgtggtcca
     1081 cgggcggcgt cacactgacg tagatccgga tctgatcggg caggctcgtc tggatgccgt
     1141 cgctcacggt gagattgaac tcgatggtcg tggcctgtgc cggcgcctgg aagctcgggc
     1201 tcgcggtaat caggtagccc acgccgttgg tcacgtcggg gcccgccgtc tgggtccagg
     1261 tgtagctcag cggcttctgg tccggatcga aggagccgga gccgttcagg accaccgtgg
     1321 cgcccgcttc gacggcctga tcgggtcccg cgttggccac cggcgcctgg cctgccgtcg
     1381 tgaacgacca cgtgtagttc tgggccaggt ggttcccggc gcgatcatag atcccggtcg
     1441 tgagggtcgc cgtgtaggtc ttctccccct ggagcgcgct cgagggggtg aaggtcacgg
     1501 tggacccggt cgccgacacc gtcccggtga ccggcgcgtc gccgaccacg acgaaggtct
     1561 gcccggtcgc cgtgctctcc gccaccgact cggagaagac cgcatggatg gcggtcccac
     1621 gatccacgcc gttggcctgg ttggagggct gggtctgggt caccgtcggg ggcgtcgtgt
     1681 cagtgcccgg tggcgggctg cctccgggcg gattcaccac aggctcggtc gcctgctttt
     1741 cgctgcagga gacgagagac agcgcgagaa gcgggacgag gaacgcgagg cgccagggat
     1801 tcgggggcat aggaaccttc gatctatcga aagcttccgg aaggaagcgg gcgtcgccaa
     1861 ggtgttaccg gcccgagaat tctgcgctcc cggattcgcc tccgtcaagc catcggcaag
     1921 aaatttttca ccgcgaacgg gcttggtagg gcgggaggcc ggggcctggc ggcctcggag
     1981 gagcccgtca ggggcgtcgg cgcccggtag cgggcccgtc ccaaacccgt tactcttccc
     2041 ccgtgccccc tgtctccaag gtcgtcgtcg tcatgcccgc gtacaacgcc gaagccaccc
     2101 tccggaagac ttacgaggag gtgatggccc agggggtcgt ggacctcgtg atcgtcgtgg
     2161 acgatcggag ccgcgacgcc acggtggagg tggcccgcac cctccccggc gtgcaggtcg
     2221 tcgttcacga gcggaacctg ggctacggcg ggaaccagaa gacctgctat cgcgcggcgc
     2281 tggcggcggg cggcgacatc gtcatcatgg tccaccccga ctaccagtac accccgaagc
     2341 tcatccccgc gatggtcgcg atgatccgct ccggtctcta cgactgcgtc gtggcctcgc
     2401 gcatcctggg cggctacgcg ctcaaggggg ggatgccgct ctggcgctac gtggccaacc
     2461 gcggcctcac gctgatcggc aatctcttca tgggagcgaa gctctccgag tatcacagcg
     2521 gctaccgcgc cttttcgcgc cgcgtgctcg agtcgctgcc gctcgaccgg aactccgacg
     2581 atttcgtctt cgacaaccag atgctggccc aggtgatctg ggcggggttc caggtggccg
     2641 aggtctcctg tcccacgcgc tacgccgccg atgcctcgtc gatcaacttc gcgcggagca
     2701 tccgctacgg attcggctgc gtcggcaccg cgatcgagtt cgccctggcg cggtgggggg
     2761 tcgcccgctc gacccacttt ccgccgccct tgcgcgggcg cgcgcggacc caggccgaga
     2821 ccctgcgcgc gtgagccccg cccggaagcg gcgacgggcg gccaccgggg gcccggccgc
     2881 gggcgtcgat cgcggggagg gacgggtcgc cc
//
LOCUS       DASSLZ010000343         6620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1749347, whole genome shotgun sequence.
ACCESSION   DASSLZ010000343 DASSLZ010000000
VERSION     DASSLZ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749347"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFM00_12790"
     CDS             complement(<1..755)
                     /locus_tag="VFM00_12790"
                     /inference="COORDINATES: protein motif:HMM:NF024426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HET7499168.1"
                     /translation="MRATATGEGVPRRSIVVASLAIALVIVAASGALAADPATAPRMT
                     SDQVADSLFGALRGGNYDAAFRLFDAKMQAAVPEEKLRTLWEEQIAQLGAFQSWQRQT
                     GDPARGYEVRLAALRFERGELKAMVAVDPLEGQVAGFFIRPASRPAAGHPAYADTTRF
                     RALEVTVGSGPLGLPGTLTLPTGSGPFPAAVLVHGSGPQDRDETIGANKVFRDIAEGL
                     ASRGVAVLRYDKRSLRYPEALKNDPTIDAEVVL"
     gene            891..1580
                     /locus_tag="VFM00_12795"
     CDS             891..1580
                     /locus_tag="VFM00_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499169.1"
                     /translation="MLIATILVALIASIIHGSFYEWAFHRYWLHRPWLPKECFTQHTL
                     IHHQLCKFDDTFHVVEEEQHEALHFQWWGGPLLIVIASVPWALLSLALHAAGVNLPYV
                     AFLITFTVGTAIYYAGYESLHYIMHKPMFPWIERTRAFRFIERHHRIHHVRMNRNLNV
                     LLPIADWVLGTLVTELEPNPVTPDSARTTARRHSRFGRRVPERGAHSRPTRAKPEGDR
                     PVWQDSNDLRS"
     gene            1550..1765
                     /locus_tag="VFM00_12800"
     CDS             1550..1765
                     /locus_tag="VFM00_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499170.1"
                     /translation="MAGFKRPSFLKKQKEQKRVAKAAEKRAARQARREAQRTDDLNPA
                     AGELVEDTGIAEEGAEATETNGEAGEA"
     gene            complement(1772..2464)
                     /locus_tag="VFM00_12805"
     CDS             complement(1772..2464)
                     /locus_tag="VFM00_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET7499171.1"
                     /translation="MRAHEERHRTGRIGWLRAAVLGANDGIVSTASLVAGVAAAHVTR
                     DAVLVSGVAGLVAGALSMAAGEYVSVSSQADTERADIAREKKELATAPEAEHAELAGI
                     YQKRGLDPDLAGQVARQLMDRDALAAHAHDELGITETLRARPLQAAIASAASFAVGAF
                     VPLATVLLIPISVLVWGVMGTALVCLAALGWLAARTGGSPVVRGTARVVLWGALAMGL
                     TAGVGALFGAAA"
     gene            2590..2955
                     /locus_tag="VFM00_12810"
     CDS             2590..2955
                     /locus_tag="VFM00_12810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HET7499172.1"
                     /translation="MGAVLGANLRYVVNRAAAQWLGTGFPYGTFAVNVAGSFAAGLVG
                     AMVTARLLGRPELVRQIVIVGFLGSLTTFSAFMYETNTLMSDGEWARALANIGLSLVA
                     GMAGVRIGVLLAARLGGAA"
     gene            2952..3320
                     /locus_tag="VFM00_12815"
     CDS             2952..3320
                     /locus_tag="VFM00_12815"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HET7499173.1"
                     /translation="MKLEGERTLLRLHLSNFLKRRGRPLYEEIVETARRERLAGATVL
                     AGTSGYFGSGPILGDHPGALAVERPVIVEIVDDSAALERFLDGVEALLRGQPVMITME
                     RARVARYGTSPAEGPEGGAP"
     gene            3317..3646
                     /locus_tag="VFM00_12820"
     CDS             3317..3646
                     /locus_tag="VFM00_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HET7499174.1"
                     /translation="MRRTREGMLLRIFIGDSDRHEGKPLHHALVERARAQGLAGATVL
                     HGPMGFGRHSRLHTAKLLELSSDLPVVIEIVDAEEAIERFMVEVDRMVGEGLVTLEKV
                     RIIELGP"
     gene            complement(3627..4841)
                     /locus_tag="VFM00_12825"
     CDS             complement(3627..4841)
                     /locus_tag="VFM00_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7499175.1"
                     /translation="MESARLIAGLTRMVRDVGLAEDLAQEALVAALERWPADGIPDNP
                     AAWLMTTAKRRAIDLLRRDKRLAPKHEEMARELESHAADSGLDDAIDDPVGDDLLRLM
                     FIACHPVLSTEARTALTLRLLGGLTTDEIARAFLVSETTVAQRIVRAKRALSEARVPF
                     EVPRGPDFVARLTSVLEVIYLVFNEGYAATAGEDWMRPALCEEALRLGRILAALMPSE
                     PEAHGLVALMEIQASRMRARIGPSGEPVLLMDQDRARWDRLLIGRGLAALARAESQGE
                     ALGPYGLQAAIAACHARARSAAETDWRRIASLYEALGRIAPSPVVELNRAVAVSMAEG
                     PAAGLAIVETLVAEPALRGYHLLPAVRGDLLEKLGRVEEARAEFSRAASITRNAVERK
                     ILLERAATGPAR"
     gene            complement(4883..>6620)
                     /locus_tag="VFM00_12830"
     CDS             complement(4883..>6620)
                     /locus_tag="VFM00_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7499176.1"
                     /translation="RPGMMTLISLAISVAFLFSLFVTFGFPGMDLWWELATLVTIMVL
                     GHWIEMRSIAQAGGALNELAKLLPDTAERLAGDGVESVPVADLREGDLLLIRPGAAVP
                     ADGVVRGGESDANEAMITGESRPVRKEAGARMIAGTVNGSGALRVEVTGTGSKTALAG
                     IMRLVAQAQSSRSRAQALADRAAFFLTVVAVAAAIATSIAWMIAGREMSYVVERVVTV
                     LVIACPHALGLAIPLVVAISTTLGAQNGLLVRDRRGLEEARNVTTVFFDKTGTLTRGE
                     FRVVDIATRDGIAPDEALALAAAVERDSEHTIARGIVKSAEERKLAIPAAGSFQALPG
                     RGVRAHVGGRTLHVGGPALLRTLGAEGERALHAATERAASRGQASVVLVENGRPLATF
                     AVADAVREESREAVRRLHEQGVEVVMMTGDARAVADAVGNELGIDQVFAEVLPDQKAA
                     RIREAQRAGKKVAMVGDGVNDAPALVSADVGIAIGAGTDVAVEAGDVILVRSDPRDVP
                     RIIALSRATYRKEIQNLWWAAGYNVAAIPLAAGVLAPWGVVLHPAVGAVLMSLSTIIV
                     AVNAQLLRRVRL"
BASE COUNT          987 a         2402 c         2287 g          944 t
ORIGIN      
        1 tccaggacca cttcggcgtc gatggtggga tcgttcttca gcgcctcggg gtagcgcagg
       61 ctgcgcttgt cgtagcggag caccgcgacg ccccgggagg cgagtccctc ggcgatgtcg
      121 cggaagacct tgttcgcgcc gatcgtctca tcgcgatcct gcggcccgga tccgtgaacg
      181 aggacggcgg ccgggaaggg gccgcttccg gtgggcagcg tgagcgttcc gggcagcccg
      241 agcgggcccg acccgacggt cacctccagc gcgcggaagc gcgtggtgtc ggcataggcc
      301 gggtgtccgg ccgcgggacg gctcgcgggg cggatgaaga atccggccac ctgtccctcg
      361 aggggatcca cggcgaccat cgccttcagc tcgccgcgtt cgaagcggag ggcggcgagg
      421 cgcacctcgt agccgcgcgc gggatcgccc gtctgccgct gccacgactg gaacgcgccc
      481 agctgcgcga tctgctcctc ccaaagggta cgtagttttt cctcgggaac ggcagcctgc
      541 atcttggcat caaagagacg gaacgcagcg tcgtagttcc cgccccgaag cgccccgaac
      601 agggaatcgg cgacctggtc gctggtcata cgcggtgctg tcgccgggtc ggccgccagc
      661 gctcccgacg ccgcgacgat cacgagggcg atcgccagcg acgcgaccac gatcgaacga
      721 cggggaacgc cctctccggt ggctgtcgcg cgcatggcgg ccatgctact cccgccggcc
      781 ccagcgcgcc accccagcag cgccccgtca ccgttctccc gtgcggtgga gcgcccttct
      841 ggagtactat gaggactcgc gctccgccct tctctgtcct ggagatccga ttgctcatcg
      901 ccaccatcct cgtcgcgctc atcgcctcga tcattcacgg cagtttctac gagtgggcgt
      961 tccaccgcta ttggctccac cgcccctggc tccccaaaga gtgcttcacc cagcacaccc
     1021 tcatccatca ccagctctgc aagttcgacg acacgttcca cgtcgtcgag gaggagcagc
     1081 acgaagcgct gcatttccag tggtggggcg gcccgctcct catcgtgatc gcttcggtgc
     1141 cgtgggcatt gctgtcgctc gcgcttcacg cggcgggcgt gaacctgccg tacgtcgcgt
     1201 tcctgatcac cttcaccgtg gggacggcga tctactacgc gggctacgag agcctgcact
     1261 acatcatgca caagccgatg ttcccctgga tcgagcgcac gcgcgccttc cgcttcatcg
     1321 agcgccacca tcgcatccac catgtgcgca tgaatcgcaa tctcaacgtc ctgctcccga
     1381 tcgccgactg ggtcctcggg accctggtga cggagctgga gccgaatccg gtcactccgg
     1441 acagcgcgcg aaccacggcg cgccgtcaca gccgcttcgg ccgccgcgtg ccggagcgcg
     1501 gagcccattc ccgccccacg cgggcgaaac ccgaaggaga ccgtccggta tggcaggatt
     1561 caaacgacct tcgttcctga agaagcagaa ggagcagaag cgcgtcgcga aggcggccga
     1621 gaagcgggcg gcgcgccagg cccgccgtga ggcgcagcgg accgacgatc tgaaccccgc
     1681 cgcgggggag ctggtcgagg acaccggcat cgccgaagag ggcgccgagg cgacggagac
     1741 gaacggtgaa gcgggcgagg cgtagccgtc cctaagccgc cgccccgaac agggcgccta
     1801 ctcccgcggt gagtcccatc gcgagggcgc cccacagcac gacccgcgcc gtgccgcgaa
     1861 ccacgggaga gccgcccgtg cgcgccgcca gccaccccag cgcggccagg cacacgagcg
     1921 ccgtccccat gacgccccag accagcaccg agatgggtat caggagaacg gtggccagcg
     1981 gcacgaacgc tccgaccgcg aagctcgcgg cggacgcgat cgccgcctga agcggccggg
     2041 cacgtagggt ctcggtgatg cccagctcat cgtgggcgtg cgcggccagg gcgtcccggt
     2101 ccatcagctg ccgcgcgacc tgtccggcaa gatccggatc cagcccccgc ttctggtaga
     2161 tcccggcgag ctcggcgtgt tcggcttccg gcgcggtggc cagctccttc ttttcccgcg
     2221 cgatgtcggc gcgctcggta tcggcctgcg agctgaccga gacgtattcg cccgccgcca
     2281 tcgacagcgc cccggcgacg aggccagcca ctccggagac cagcacggcg tcgcgcgtca
     2341 cgtgcgccgc cgccactccg gccacgaggc tcgccgtgga gacgatcccg tcgttggctc
     2401 ccagcacggc ggcgcgaagc catccgatcc gcccggtgcg atgacgctct tcgtgagcac
     2461 gcatagccgc tcctttccgg ttcgcgattc cgatacgatg gacgaccata ccaccggacc
     2521 atcgtcgcgt cgttttccta tggaaggagg ttcgtcatcg agcgcgtctc cgagttcctc
     2581 tggatctccc tgggggccgt tctcggcgcc aacctgcgct acgtcgtgaa tcgcgcggcc
     2641 gcgcagtggc tggggacggg gtttccctac ggcacgttcg cggtcaacgt cgcggggagc
     2701 ttcgccgcgg gcctggtcgg cgccatggtc acggcccggc tcctcgggcg ccccgagctg
     2761 gtgcggcaga tcgtgatcgt cggcttcctc gggtcgctca ccacgttctc cgcgttcatg
     2821 tacgagacga acaccctgat gagcgacggc gaatgggcgc gggccctggc gaacatcggg
     2881 ttgagcctgg tcgccggcat ggccggcgtt cgcatcggcg tcctgctcgc ggcgcggctc
     2941 ggaggtgcgg catgaagctc gaaggggaaa ggacgctgct gcgcctccac cttagcaatt
     3001 tcctgaagcg ccggggacgg ccgctctacg aggagatcgt cgagacggcc cgccgggagc
     3061 gcctcgccgg cgccacggtg ctcgcgggga cgtcggggta tttcgggagc ggccccattc
     3121 tcggcgacca ccccggcgcc ctggcggtcg agcggcccgt catcgtggag atcgtggacg
     3181 attccgccgc gctcgagcgg tttctcgacg gcgtcgaggc gctgctccgc ggtcagccgg
     3241 tgatgatcac gatggagcgg gcgcgcgtcg cgcgctacgg aacgagcccg gccgagggac
     3301 ccgaaggggg cgcgccatga gacggacccg tgaagggatg ctgctccgga tcttcatcgg
     3361 ggattccgat cgccacgagg ggaagccgct ccatcacgcg ctggtcgagc gtgcgcgcgc
     3421 gcaggggctg gcgggtgcga cggtgctcca cggtcccatg ggattcggcc gccactcgcg
     3481 cctccacacg gcgaagctgc tggagctgtc ctccgacctg ccggtcgtga tcgagatcgt
     3541 ggacgcggaa gaggcgatcg agcggttcat ggtggaggtg gaccggatgg tgggggaggg
     3601 gctggtgacg ctggagaagg tccggatcat cgagctgggc ccgtagcggc ccgctcgagc
     3661 aggatcttcc gctcgacggc gttccgcgtg atcgacgcgg cccgcgaaaa ctccgcgcgc
     3721 gcctcctcga cgcgccccag cttctcgagg agatcgccgc gcaccgccgg caacaggtga
     3781 tagccgcgga gcgccggctc ggcgacgagc gtctcgacga tggcaagccc cgccgcgggc
     3841 ccctcggcca tcgagaccgc gaccgcgcgg ttcagctcca ccacggggga aggggcgatc
     3901 cggccgagtg cctcgtagag cgacgcgatg cgcctccaat cggtctccgc cgcggaccgc
     3961 gcgcgcgcgt ggcaggccgc gatggcggcc tgcagcccgt agggtcccaa cgcttcgccc
     4021 tgcgactcgg cccgcgcgag cgccgcgagg ccgcggccga tgaggaggcg gtcccaccgc
     4081 gcgcgatcct gatccatcag gagcaccggc tcgcccgagg gtccgatccg cgcccgcatc
     4141 cgcgacgcct ggatctccat caaagcgacc agcccgtgcg cctcgggctc cgagggcatg
     4201 agcgccgcga ggatccgtcc caggcgcagc gcctcctcgc agagcgcggg gcgcatccag
     4261 tcctcgccgg cggtcgcggc atagccctcg ttgaagacca gatagatcac ctcgagcacc
     4321 gacgtaagcc gcgccacgaa gtcgggcccg cgcgggacct cgaaggggac gcgcgcctcg
     4381 gaaagggcgc gcttggcgcg gacgatccgc tgcgccaccg tcgtctcgga gaccaggaag
     4441 gcgcgcgcga tctcgtcggt cgtcagcccg ccgagcaatc gcagcgtgag ggccgttcgc
     4501 gcctcggtgg agagaaccgg atggcaggcg atgaacatga gccggaggag gtcgtcgccg
     4561 accggatcgt cgatggcgtc gtccagcccg gaatccgcgg cgtgggattc gagctcccgg
     4621 gccatctcct cgtgcttggg cgcgagccgc ttgtcgcggc gcaggagatc gatggcgcgc
     4681 cgcttcgccg tggtcatcag ccaggccgcc ggattgtcgg gaatgccgtc cgccggccag
     4741 cgctcgaggg ccgcgacgag cgcttcctgc gcgagatcct ccgccaggcc cacgtcgcgc
     4801 accatccggg tcagtcccgc gatgaggcgg gccgactcca tgtgccagac cgcttcgatc
     4861 gcgcgccgga cgtcgggagg tgtcacaggc ggacgcgccg caggagctgc gcattgacgg
     4921 cgacgatgat cgtgctgagg gacatgagca cggccccgac ggcgggatgg aggacgacgc
     4981 cccagggagc gagcaccccc gcggcgaggg gaatggcggc gacgttgtag ccggcggccc
     5041 accagagatt ctggatctct ttccggtagg tcgcgcggct cagcgcgatg atgcggggga
     5101 cgtcgcgcgg atcgctccgg accaggatca cgtcccccgc ctccacggcg acgtcggtgc
     5161 cggctccgat cgcgatgccg acgtccgcgc tcacgagcgc gggcgcgtcg ttcacgccgt
     5221 cgcccaccat cgccaccttc ttgccggcgc gctgcgcctc ccggatgcgc gcggccttct
     5281 gatcgggcag cacctcggcg aacacctggt cgatccccag ctcgttcccc acggcgtcgg
     5341 cgaccgcgcg cgcgtcgccg gtcatcatca ccacttcgac cccctgctcg tgaaggcgcc
     5401 gcacggcctc gcgcgactct tcccggacgg cgtcggccac ggcaaaggtc gcgagcggcc
     5461 ggccattctc caccagaacc accgacgcct ggccccgcga ggcggcgcgc tccgtcgcgg
     5521 cgtggagtgc ccgctccccc tcggctccca gcgtgcggag cagcgccggc ccgcccacgt
     5581 gtagagtccg cccgcccacg tgagcccgca ccccccggcc ggggagggcc tggaacgagc
     5641 cggccgccgg gatcgcgagc ttccgctcct ccgcgctctt cacgatcccg cgcgcgatgg
     5701 tgtgctccga atcgcgctcc accgccgcgg ccagggcgag cgcctcgtcc ggtgctatgc
     5761 cgtcgcgcgt ggcgatgtcc accacccgga actcgccgcg ggtcagggtg cccgtcttgt
     5821 cgaagaagac cgtcgtcacg ttccgagctt cttcgaggcc ccgccggtcc cgcaccagga
     5881 gcccgttctg cgcgcccagg gtcgtggaga tcgcgaccac gagcgggatc gcgaggccca
     5941 gcgcgtgcgg gcaggcgatg acgagcaccg tgacgacccg ctccaccacg tacgacatct
     6001 cacgcccggc gatcatccag gcaatcgagg tggcgatcgc ggcggccact gccacgaccg
     6061 tgaggaaaaa cgccgcgcga tcggcgagcg cctgcgcgcg ggagcgcgag ctctgcgcct
     6121 gcgccacgag gcgcatgatc cccgccagcg ccgtcttcga tccggtcccg gtgacttcga
     6181 cccgcagcgc gcccgaaccg ttcacggttc cggcgatcat acgggcgccg gcctccttcc
     6241 gcaccggcct cgattcgccg gtgatcatcg cctcgttcgc gtcgctctca ccgccgcgaa
     6301 cgacgccgtc cgcgggaacc gcggcccctg gacggatcag gagaagatcc ccctcgcgga
     6361 gatccgccac gggcacgctc tcgaccccgt cgccggcgag gcgctcggcg gtgtcgggca
     6421 ggagcttggc cagctcgttc agggcgccgc cggcctgggc gatggagcgc atctcgatcc
     6481 agtgcccgag caccatgatc gtgaccagcg tggcgagctc ccaccagaga tccatcccgg
     6541 ggaagccgaa ggtgacgaac aggctgaaca ggaaggcgac gctgatcgcg agcgagatca
     6601 gcgtcatcat gccggggcgt
//
LOCUS       DASSLZ010000344         4354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_882263, whole genome shotgun sequence.
ACCESSION   DASSLZ010000344 DASSLZ010000000
VERSION     DASSLZ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882263"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1269)
                     /locus_tag="VFM00_12835"
     CDS             complement(85..1269)
                     /locus_tag="VFM00_12835"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HET7499177.1"
                     /translation="MLLEAPLLNPAELKVLDQIDDLRRKLRFALSPPTRWKGLIRRAT
                     LAKAIRGSNTIEGRTISFDDALALTAGDGPFETDEGATAAMVGYQRAMNYLLRLSVDP
                     VFRYSSDLLRSLHYMMLEHDLEKAPGQWRSGDFFVRGTSGNEIVHYGPDPDEVPGLMD
                     ELVARLNANHSSTHSIVDAAMAHLNLVLIHPYVDGNGRMARALQTLVLARSGVLEPVF
                     SSIEEYLGRNTLEYGAVLAAVRGDRWDPHRDAREWIRFCLTAHYRQATTLLRRIERAR
                     AVWDRAEQEVSKRKLPERTILALVDATLGYRVRNATYRSAACISDHLASLDLKALVGS
                     GLLVPHGERRGRFYLAADCLKSIRAATRPKKPVEDPFEGSASGQLMPWPPAQRRALDR
                     RG"
     gene            complement(1269..2069)
                     /locus_tag="VFM00_12840"
     CDS             complement(1269..2069)
                     /locus_tag="VFM00_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET7499178.1"
                     /translation="MTPVEIIRVAVMDDDDTERQRLVHHLRAESDLEIVGVLSGGRRA
                     VSILKRLAPDVLFLDVHLRELDGFEVLRSMNGAGPPNVVFVTAAPEHAAQAFDLGAVD
                     YLLKPIESSRVAQAVARVRAVLRRERATQLLSSLERLLHRQADPDAHGSPAPSLERFM
                     VHDRERSYFLKTSEVDWIEAADNYVRVHTGGTTHLIRQTLTQFHAKLDPTRFVRIHRS
                     TVVNLDRVREIQPGVGRDRLVVLECGTQLRMSERYRVNLDHLSRRGLS"
     gene            complement(2188..3036)
                     /locus_tag="VFM00_12845"
     CDS             complement(2188..3036)
                     /locus_tag="VFM00_12845"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7499179.1"
                     /translation="MMNLREWKPIPGLEDFVERVWLLEVPAGGHQWHRVFPDGRIDIT
                     FGLRDRVLRRTPSGQVVLQPRACVIGQRSEPLWLEPTGRWRTAGIRLRPEGAYAVLNM
                     NLAELSDSSVPLDELWGPLGKEIVAKVSEAKTARDALRQFQEFLLERLRTTRRPHPGT
                     VRAVQALVESRGQEPVHRLARSVGWSERTLERRFAREVGLSPKRLAQTVRFQSLLASA
                     SAERKESWASLAWDCGFADQAHLTREVRRFAGASPTKLEHGELALARLFLSRERLRSY
                     FRDRPG"
     gene            complement(3180..>4354)
                     /locus_tag="VFM00_12850"
     CDS             complement(3180..>4354)
                     /locus_tag="VFM00_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7499180.1"
                     /translation="RGRAIGTWSGFSGITAAIGPLLGGWLVNHSWRWAFFINAPLAVV
                     VLLLLAKVPESRSPRPQGLDAIGAVLVTIGLGGLVYGLIESSQRGWGEASVITALACG
                     CGGLAAFVFREARTSSPMLPLRLFRSADFTGTNALTLFLYGALACAFFFLPLDLIQVQ
                     GYSPLQAGAALLPFIVILFLLSRWSGGLVERYGPRKPLLIGPAVAALGFVLLGLPGIG
                     GSYWRTFFPGVLVLGLGMAASIAPLTTAVMNAVGSESSGIASGVNNAVSRAAGLLSIA
                     LLGILITSVFDRELDRRVAALPLDPGVRHQVVSERGKLAAAEPPRGLSEEQAHAVRVA
                     IGASFVEAFRAVCRACCVLALLAAACAWVWIGRGEALKRASGETNRLPTISRSSVS"
BASE COUNT          756 a         1447 c         1494 g          657 t
ORIGIN      
        1 actcttggga ccatcgatgg gtttcgaggg gtgaagacca cgaagcgcgg ggcgtccgcg
       61 tggctcctcg catcgacggg cacgtcatcc ccgtcgatcg agggcccgcc tctgtgcggg
      121 cggccaaggc atcagctggc ccgatgcgga tccttcgaac gggtcttcca cgggtttctt
      181 ggggcgggtc gctgcccgga tggacttcag gcagtcggcc gccagataga aacggcccct
      241 ccgctcgcca tgcgggacca gcagtccgga ccccacgagg gccttcaggt ccaggctcgc
      301 caagtggtcg gagatgcagg ccgcgctgcg atacgtggcg ttccgaacgc ggtaccccag
      361 cgtcgcgtcc acgagcgcca gaatcgtccg ttcgggcagc ttccgcttcg agacttcctg
      421 ctccgcgcga tcccacaccg cccgagctcg ctcgatgcgc cggagcaggg tcgtcgcctg
      481 gcggtagtgc gcggtcaggc agaagcggat ccactccctc gcgtcacggt gcggatccca
      541 gcgatcgccc ctcaccgcgg ccaggacggc gccgtattcg agcgtgttcc tgccgaggta
      601 ttcctcgatg ctcgagaaga cgggctcgag gaccccggac cgcgccagga ccagcgtttg
      661 aagcgcccga gccatccgtc cgttgccgtc gacatagggg tggatcagga ccaggttgag
      721 atgggccatg gccgcgtcca cgatgctgtg cgtggaggag tggttggcgt tcaggcgtgc
      781 gaccagctcg tccatcagcc cgggcacctc gtcgggatcg ggcccgtagt ggacgatctc
      841 gtttcccgac gtgccgcgaa cgaagaagtc tccgctcctc cactgacccg gggctttctc
      901 gaggtcgtgc tcgagcatca tgtagtggag gcttcgcaac agatcgctcg agtagcggaa
      961 gaccggatcg acggagagcc gcagaaggta gttcatggcg cgctggtagc ccaccatcgc
     1021 cgccgtcgcg ccttcatcgg tttcgaaggg tccatccccc gcggtcaggg ccagcgcgtc
     1081 gtcgaacgag atcgtgcgcc cctcgatcgt gttggacccg cggatggcct tcgcgagcgt
     1141 cgctctccgg atcaggccct tccatcgtgt ggggggactc aaggcaaagc gcagcttgcg
     1201 cctcaagtcg tcgatctggt cgaggacctt gagctcggcg ggattcagga gcggggcctc
     1261 gaggagcatc aggaaagccc cctgcggctc agatggtcca ggttcacccg gtagcgctcg
     1321 ctcatgcgca actgggtgcc gcattccagg accacgagac gatcgcgtcc gaccccgggc
     1381 tggatctccc gaacgcggtc cagattgacg acggtgctgc gatggatccg cacgaatcgg
     1441 gtcggatcga gcttggcgtg gaactgcgtc agcgtctggc ggatgaggtg cgtcgttcca
     1501 cccgtgtgga cccgcacgta gttgtccgcg gcttcgatcc agtcgacctc cgatgtcttc
     1561 aggaagtagg accgttcccg gtcatggacc atgaaccgct cgagcgaggg ggccggcgat
     1621 ccgtgcgcgt cggggtcggc ctggcgatgc aacagtcgct cgagggagga caggagctgc
     1681 gtggcccgct cgcgccggag cacggcccgg accctcgcga cggcctgcgc gacgcgcgag
     1741 gattcgatgg gcttgaggag gtagtcgacg gctccgagat cgaatgcctg cgcggcgtgc
     1801 tcgggggcgg cggtcacgaa gaccacgttc ggcggccccg ccccattcat ggagcggagg
     1861 acctcgaaac cgtccagctc tcgaagatgg acgtcgagaa agaggacgtc gggagcgagc
     1921 cgcttcaaga tcgagacggc ccttcgcccg ccggatagga cgcccacgat ctcgaggtcg
     1981 gactccgccc tcagatggtg caccagtcgc tggcgctcgg tgtcgtcgtc gtccatgacc
     2041 gcgacacgga tgatttccac tggagtcatc aggctcggcg acgtctcgag ccggcggtca
     2101 ggcgcccggg ggggaggcgg gcacagcaga gattagtgga gccgattcgc tcggtattga
     2161 agaaacccga cagtgacgct cgcgatgcta gccgggcctg tcacggaaat aggaccggag
     2221 ccgctcccgc gacaggaaca ggcgggccag ggcaagctcc ccgtgttcca gcttggtcgg
     2281 tgacgcgccg gcgaaccgtc gcacctctct cgtgaggtgc gcctgatcgg cgaatccaca
     2341 gtcccaagcc aacgaagccc aactctcttt tcgttccgcg gacgcgctgg cgaggaggct
     2401 ctggaatcgc accgtctggg ccagccgctt cggactgagg ccgacttccc tggcgaaccg
     2461 gcgctccagc gtccgctcgc tccagccgac ggaccgggcc agccgatgaa ccggctcctg
     2521 gccgcgcgat tcgacgagtg cctgaaccgc gcgaacggta cccggatgag gccttcgggt
     2581 cgtgcgcagt cgctcgagga gaaactcctg gaattgccgg agcgcgtctc gggcggtctt
     2641 cgcctcggag accttggcca cgatttcctt gccgagcggg ccccaaagct cgtcgagtgg
     2701 gaccgaggaa tccgacaact ccgccaagtt catgttcagg acggcgtacg ccccttccgg
     2761 tcgaagccgg atgccggcgg tcctccagcg tcccgtgggc tccagccaga gcggctcgga
     2821 tcgctggccg atcacgcagg cccggggctg cagcacgacc tgcccgctgg gggttcgtct
     2881 caggacgcgg tcccgcaagc cgaaggtgat gtcgattcgc ccgtccggaa aaacgcgatg
     2941 ccactgatgg ccgccggccg ggacctcgag cagccagaca cgctcgacga agtcctcgag
     3001 ccccggaatc ggcttccatt cacgcaggtt catcatggct ccccgcgaag gcatgcaacg
     3061 gaatgctcgc cccaagcgag ccgcggatgg ggcgcagttg gtgtgctgtt ggatgctgcg
     3121 gtgttcgatc caggtcccga acaatccctc ggggccggca ctctgggggg gtactgcctc
     3181 taggatacgc tggaccgtga gatggttgga agacggttgg tctccccgct cgcccgcttc
     3241 aacgcttcgc cgcgcccgat ccagacccat gcacaggccg cggccagcag cgccagaacg
     3301 cagcacgcgc ggcagaccgc ccggaacgcc tcgacgaacg aagcgccgat cgcgacccgg
     3361 accgcgtggg cctgctcctc cgaaaggccg cggggcggct cggccgccgc cagtttgccg
     3421 cgttccgaaa ccacctggtg gcgaaccccg gggtccagcg gtagcgccgc gacacgccga
     3481 tcgagctcgc ggtcgaagac gctcgtgatc agaatcccga ggagggcgat ggagaggaga
     3541 cccgcggcgc gcgacaccgc gttgttcacg cccgaagcga tcccggagct ctccgaaccg
     3601 acggcgttca tcacggcggt cgtgagcggg gcgatgctcg cggccatacc caggcccagc
     3661 acgaggaccc ccggaaagaa cgtgcgccag tacgagccgc cgatcccagg caggccgagg
     3721 agaacgaaac cgagcgccgc gacagccggg ccgatgagca gcggcttccg cgggccgtag
     3781 cgctccacca atccgcccga ccatcgcgac agcaggaaga gaatgacgat gaatgggagc
     3841 agcgcggcgc ccgcctgaag cggcgaatat ccctggacct ggatcaggtc caggggcaga
     3901 aagaagaagg cgcaggcgag ggctccgtag agaaagagcg tgagggcgtt cgttccggtg
     3961 aaatcggcgg agcggaacag gcggagcggg agcatcggcg atgacgttcg cgcctcccgg
     4021 aagacgaatg ccgcgagccc tccgcatccg cacgcaagcg ctgtgatcac cgacgcctcg
     4081 ccccatcccc gttgcgagga ttcgatgaga ccgtagacca gcccgcccag cccgatcgtg
     4141 accagaacgg cgccgatcgc gtcgagtccc tggggccgcg gactccggct ctcgggcacc
     4201 ttcgcaagga ggaggagaac gacgaccgcg agcggagcgt tgatgaagaa ggcccaccgc
     4261 caggagtgat tcaccagcca gccgccgagc agggggccga tggccgcggt gatcccgctg
     4321 aatccggacc acgtgccgat ggcgcgcccg cgct
//
LOCUS       DASSLZ010000345         3557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_124817, whole genome shotgun sequence.
ACCESSION   DASSLZ010000345 DASSLZ010000000
VERSION     DASSLZ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124817"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..1136
                     /locus_tag="VFM00_12855"
     CDS             354..1136
                     /locus_tag="VFM00_12855"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7499181.1"
                     /translation="MKLPRQTDQALLRAFLVDRLAPRRGERILDLGCGFGLELEEILR
                     RHRTVRVVGLDSSERMIDVARRRLSRYARSGRAELVVGDAGEKLPFPSKHFDAVLSVE
                     LIECLPPVKRTQLLKEIRRVLVPGGRVLMEHTDWDTQVWNAKDKALVRKLVHAFCDWT
                     QGWMATSDGWMGRRLPGLFREAGCFRDLAIAVHVLTNDRFQRGTYGYDRAQDFLSLAK
                     AKKGITLAKARRFLRDLKAREREGAYFYSVTRFVVTGERTRR"
     gene            1369..3309
                     /locus_tag="VFM00_12860"
     CDS             1369..3309
                     /locus_tag="VFM00_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET7499182.1"
                     /translation="MTESRRKKVGEYLLEAGLVTEDQLKEALRRQRQTKEPLGQILAR
                     TGFVAEADVCRVLHQQLGLPIVDLQALAIDEAVIALVKEELAKRYTAIPVGKENQNTI
                     RVAMADPLNASAMEDIRFQSGYFVRPVLAPPTEILEAITRYYHLDDSVGEVLENIIKS
                     DPGREVRELVEDAQEESLTANAAELAAGSPIVRLANWIIARAVELRASDIHIEPQDKG
                     VGVRVRVDGLLQDLERMPKWTQGALISRIKILAALDIAEKRVPQDGRFRVESEGRRID
                     LRVSTLPTLHGEKIVIRIVDQERTALRLDTLGLTPESLADIREFSGRPQGILIVTGPT
                     GSGKTTLLYSILEQIHSVTKNIITAEDPIEHQVPGVNQVQVDEKSKKTFASVLRAMLR
                     QDPDVMMVGEVRDAETAQIAFRASITGHLVLSTVHTNDAASAVTRLVDLGLAPYMVSS
                     ALIAVISMRLVRTLCPKCKDAYVPSADLLRSAGLSGNDARRLVLYRPSGCEACNGTGY
                     RGRTGIYEILEMRESLRRLVTSGATEAMIRAAAIEAGMVPIGQDGLSKALAGETTIDE
                     LRRVIYTPDEAGRICGGCHATISAEYSFCPECGTRAELACEQCGKRVAPEWSYCTGCG
                     HPRPGAVSDVEPGALDPSSFAP"
BASE COUNT          591 a         1184 c         1204 g          578 t
ORIGIN      
        1 gggccaaagc ctaaaacact cgacggccgt gcctactggc ggccgagcgc tacgcgtttg
       61 gcgcgagggg aggcagccct ccgggcgcgc cgccctccgg cccgactctc atcgctcgtt
      121 tggtagcctc ctcggcgggc ctccgggagg gcgccctccg cggctgtctc ccctcgcgtc
      181 caaggatcgc gtcgcgacct gccgacgcgt gctcgtcata cgattttcag tttgggagac
      241 ccgcggccac ttttttaccg ccgctagcgg cggtgaatcg atggacaagt gtcttcatga
      301 agtgacgcga cagaaggtgg cgctccttga agagtgcgag gccggcaatt ttcatgaagc
      361 tacctaggca gacggatcag gcgctcctgc gtgcgtttct cgtcgatcgg ctcgcacctc
      421 ggcgagggga gcgaattctc gacctcggct gtggattcgg cctggagctg gaggaaatcc
      481 tccggcgtca ccggactgtg cgggtcgttg ggctggattc atccgaacga atgatcgatg
      541 tcgctcgacg gagattatcc cgctacgcac gtagcgggag ggctgagctc gtcgttggag
      601 atgcaggcga aaagctcccg ttcccgtcga agcatttcga tgcggttctc tccgtcgagc
      661 tcatcgagtg cctgccgccg gtaaagcgca cgcagctcct caaggagatc cgtcgcgttc
      721 tcgtgcccgg ggggcgcgtc cttatggagc acacggactg ggacacacag gtctggaacg
      781 ccaaggacaa ggcgctcgtg cggaagctcg ttcatgcgtt ctgcgattgg acccaggggt
      841 ggatggcgac ctccgacggg tggatgggcc gtcggctccc cggtctgttt cgagaggccg
      901 gatgcttccg tgacctcgcg attgctgttc acgttctgac gaacgaccgg ttccaacgcg
      961 ggacctacgg atacgaccgg gctcaggact tcctctcgct cgccaaggcc aagaagggaa
     1021 tcaccctcgc gaaggcccgg cggttcctgc gcgacttgaa ggcaagggaa cgagagggcg
     1081 cgtacttcta cagcgttacc cgcttcgtcg tgaccggaga gcggacccgc aggtaggatc
     1141 gaaccctggt ccctgggcta ctggcaagct ctcgaccgtc ccctcaccaa gatcgcctgc
     1201 ttttggccgc tgcgaactcc atgctcgttt tccgcggtag tttctaaccc ataacacggt
     1261 cgtcggtttg gctggagaag gtcaggtctt tgcagtggca cgacgattgc cctttcggtc
     1321 gcggccggat ctcgtccttg gccggcgcca ccctttgggg atcgatcggt gaccgagagc
     1381 aggcggaaga aggtgggcga gtacctcctc gaggcgggcc tcgtcacgga ggaccagctc
     1441 aaggaggcgc ttcggcgcca gcggcagacg aaggagccgc tggggcagat cctcgcgcgc
     1501 acgggcttcg tcgccgaagc cgacgtctgc cgcgtcctcc accagcagct cgggcttccg
     1561 atcgtggacc tccaggcgct cgccattgac gaggcggtga tcgcgctggt caaggaggag
     1621 ctggccaagc gctacaccgc gatcccggtc ggcaaggaga accagaacac gatccgggtc
     1681 gcgatggccg atccgctgaa cgcctcggcc atggaggaca tccgcttcca gagcggctac
     1741 ttcgtgcgcc cggtcttagc accccccacg gaaatcctcg aagcgatcac gcgctactac
     1801 catctcgacg actcggtcgg ggaggtgctc gagaacatca tcaagagcga cccggggcgc
     1861 gaggtgcggg agctggtcga ggacgcgcag gaggaatcgc tgacggcgaa cgcggccgag
     1921 ctggccgcgg gctcgccgat cgtgcgtctg gcgaactgga tcatcgcgcg cgcggtcgag
     1981 ctccgagcga gcgacatcca catcgagccg caggacaagg gcgtgggcgt gcgcgtgcgc
     2041 gtggacggct tacttcaaga tttggagcgg atgccgaagt ggacgcaggg tgccttgatc
     2101 tcgcgcatca agatcctcgc cgccctcgac atcgccgaga agcgggtgcc ccaggacggc
     2161 cgcttccggg tggagtcgga ggggcgccgc atcgatctcc gcgtctccac gctgccgacc
     2221 ctgcacggcg agaagatcgt gatccgcatc gtggaccagg agcgcacggc gctccgcctg
     2281 gacacgctgg ggctgacgcc ggagagcctg gcggacatcc gggaattttc cggaagaccg
     2341 cagggaatcc tgatcgtcac cggtccgacg ggaagcggca agaccacgct cctctactcg
     2401 atcctcgagc agatccactc ggtcaccaag aacatcatca cggccgagga cccgatcgag
     2461 caccaggtcc cgggcgtgaa ccaggtgcag gtggacgaga agagcaagaa gaccttcgcc
     2521 tcggtgctcc gcgccatgct gcgccaggac cccgacgtca tgatggtggg cgaggtgcgc
     2581 gacgccgaga cggcgcagat cgcgttccgc gcctcgatca cgggacacct ggtcctctcg
     2641 acggtgcaca cgaacgacgc cgcctccgcc gtgacgcggc tggtcgacct ggggctcgcc
     2701 ccctacatgg tctcctccgc gctgatcgcg gtgatctcga tgcgcctggt ccggacgctc
     2761 tgtcccaagt gcaaggacgc ctacgttccc tcggccgacc tgctgcggtc ggcgggcctc
     2821 tccggcaacg acgcgaggcg cctggtgctc taccgcccgt cgggatgcga ggcgtgcaac
     2881 ggcaccggct accgtgggcg cacgggcatc tacgagatcc tcgagatgag ggaatcgctc
     2941 cgccggctgg tcacctcggg cgccaccgag gcgatgatcc gcgccgccgc catcgaggcg
     3001 ggcatggtgc cgatcggaca ggacgggctc tccaaggcgc tcgcgggcga gacgacgatc
     3061 gacgagctgc gccgcgtgat ctacaccccc gacgaggcgg ggcgcatctg cggcggctgc
     3121 cacgccacca tctcggccga gtactccttc tgtccggaat gcgggacgcg cgccgagctc
     3181 gcgtgcgagc agtgcggcaa gcgcgtcgcg ccggagtggt cgtactgcac gggatgtgga
     3241 cacccgcggc cgggcgccgt cagcgacgtc gagcccggcg cgcttgaccc gtcctcgttc
     3301 gctccctaga atcccccgac ttcccgcacg ctgcggcaac gtgccgcgcc cggaggcggc
     3361 gggagcgcac acgatcgcgg gagccccgtg tccattcagg aagtccgccg cgcgaagctc
     3421 caggcgctcc aggagcgcgg gatccagccg tacgcctacc gctacgacgc cacgcaccgc
     3481 tccgccgcga tccgcgcgga tcacgccgcc ctggaggcct cggggcagcc ggtgcgcgtc
     3541 gccgggcgtt tgatgac
//
LOCUS       DASSLZ010000346        22186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1135600, whole genome shotgun sequence.
ACCESSION   DASSLZ010000346 DASSLZ010000000
VERSION     DASSLZ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 22186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22186
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135600"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VFM00_12865"
     CDS             complement(<1..1148)
                     /locus_tag="VFM00_12865"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7499183.1"
                     /translation="MGAALQGSTDVRIAAARLRAARGAARRQRGAFDPELFGNVNHLE
                     GEQRQTSIFSGTKSLQTFLTGGARITLPFGTQLSATLDADRVDDNAPVNTLRPRYDAA
                     GRISLRQPLLRGFGPGTWSERTATNREQEAAQARYDDAVSLVRANVERTYWEVYAAER
                     DLAVAQLIRDQATALLNQAQLRSRAGLVGPGQVATAQVFLAEQEQAVLDRQEALDQAS
                     DRLASQIGERPSSEPARFHPSDEPPRDFPVEPEGDLVTRALQSNPELRARERDVAAAR
                     ARHTGARWNAYPQLDVFGTLGGTGLRGDPRTVIFGADTVTTTVPQGTIGDAISEATAR
                     DFPGWSAGASVAVPIGVRAGAGERDRLQAEADRAQANLVAGQRSLADDV"
     gene            complement(1461..2483)
                     /locus_tag="VFM00_12870"
     CDS             complement(1461..2483)
                     /locus_tag="VFM00_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7499184.1"
                     /translation="MAQREPSGDSFEMEPLPEFEARQHPAPLDAYSRAVIHAAAVAGP
                     SVVSIEVRHPEPGPEARGRRPMPRGGSGSGFVFTPDGFILTNSHVVHGASSIVVHLPE
                     GGRHEAQLIGDDPESDLAVIRIHAPNLTPATLGDSQSLLVGQLVIAVGNPFGFQATVT
                     AGVVSALGRSLRSGSGRLIDNVIQTDAALNPGNSGGPLVTAQGEVVGVNSAVILPAQG
                     ICFAIAVNTAKRVASQLIQFGRVRRSVIGIAGQNVTLPRKVARYFRLPVESGVLIQSL
                     QPNGPAERAGAREGDVILGFGEDPVASVDDLQAKLIDQAAGVPRSLSVLRGVELQTLE
                     VVPAEA"
     gene            2635..3489
                     /locus_tag="VFM00_12875"
     CDS             2635..3489
                     /locus_tag="VFM00_12875"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7499185.1"
                     /translation="MTPSTEFYLGDPEDHGRLRCLVWEVERPRGRISIIHGLGDHAAR
                     YTHVAEALAGRGFSVEAMDLPGHGKSGGPRGHVRHWDDYRGAVETWMRHSASLPGAET
                     RAILGQSMGAFVALDWTLGHPREVRALVLCGAPFEVVLKPSMLKVKAAQLAARIWPGF
                     SQGNTILPSMLSRDPEVVRAHSVDPLVHYKISARLFFEFQRMRLELMRRAAELPVDTL
                     VIHGGADPISSVVGAQRWAARCPQGRVTCRLYPGLLHEVLNELERREVMEDVVGWVEG
                     VMPGAGAA"
     gene            complement(3494..4969)
                     /gene="glpK"
                     /locus_tag="VFM00_12880"
     CDS             complement(3494..4969)
                     /gene="glpK"
                     /locus_tag="VFM00_12880"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET7499186.1"
                     /translation="MKLLLAIDQGTTGTTVLLLDDRARVTARGYAELPQHFPRPGWVE
                     HRGEEIWDTTLRALRAALRGARAERIAAIGITNQRETAMLWDRRALRPAAPAIVWQDR
                     RTAERCEALRRAGHESWVRKRTGLRLDPYFSATKVEWLLAHDRDLRARARRGAIAFGT
                     VDSWLVAKLTGGAVHATEPTNASRTLLYDIRRRRWDPELAALFGVPEVVLPEVRPSAG
                     DFGSTRGVPGLPDGIPIRGIAGDQQAALFGQGCVRRGGIKNTYGTGCFLMLHTGAAPT
                     ASRSGLLTTAACGPRGEPAYALEGSVFIAGAAVQWMRDGLGIIATSSEVEALARSVPD
                     AGGTVLVPAFAGLGAPWWRPDARGVWSGLTRGTTRGHLARAALESIAFQTCDVVSAME
                     KDARARVRALRVDGGAAANDLLMQTQADLLGIPITRPAMLESTALGAALLAGIGAGIW
                     KDRADVPAARSGERVFRPRMAGAERRRRLASWRMAVERLLD"
     gene            complement(4966..6477)
                     /locus_tag="VFM00_12885"
     CDS             complement(4966..6477)
                     /locus_tag="VFM00_12885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET7499187.1"
                     /translation="MVDILVIGGGIHGVAVARDAARRGLETLLLEKGDLASATSSRTS
                     KLIHGGLRYLENGQIRLVREALRERAILLHTAPEFVRPLPFLLPHYAGRGRARWWVGA
                     GLALYRLLAGGTAAERSGIGAEEALRLAPGLDPEDLRGGSLFADAQMDDAALCVALAV
                     DAERAGATVRTHTEAIALAKTADGRAWRAHFHDRIEGTDAEVEARAVVNAGGPWVDDV
                     RAMAEGHPAPSVRRTRGAHVVFAAAPPRTALLLTARRDGRVFFVIPWGAHTLAGTTDV
                     DDPSDPGSVEPHVEDVRYLLEESAAALPGALAGVRPVRAFAGVRPLSRGRAERPWENS
                     RESRVLVERGLWTMVGGKYTTHRALAERVVNGIVRALGARVAPCVTASLPLRSGRAES
                     IERLRRDFPRAAEVAGGFHISEAEVAHAARVERARRLDDILLRRTRLWLDARALREAA
                     MPCAVWAAPVLGWSERTRSAEVERLLAILDREERAIDAAVGSGHAEPGTRGIA"
     gene            6625..7413
                     /locus_tag="VFM00_12890"
     CDS             6625..7413
                     /locus_tag="VFM00_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7499188.1"
                     /translation="MAPLAPLEGRRALVTGATSGFGLAIARRFASEGARVVVTGRRED
                     RLRAVADELRAKGADATALVFDVRDRAAVERALSDRALVESLDIVVNNAGLAAGLDPV
                     QTADPEDWERMIDTNVKGLLWVTRAVLPAMIAKTRGDVVNIGSVAGHQAYGGGSVYAA
                     TKFAVRAITEALRYDVLGTGVRVMNVEPGLAETEFSLVRFHGDQARAKAVYRGVRPLT
                     AEDVADAVAWAVTRPAHVNVQEILLMPTDQASAYAVHRREEPAR"
     gene            7446..8285
                     /locus_tag="VFM00_12895"
     CDS             7446..8285
                     /locus_tag="VFM00_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7499189.1"
                     /translation="MPIPSRTRFGAATAALALVLAASALAGKPAPLAVQESGSGSPTI
                     VLVHSIGGDRNDWSAVAPRLAEHHRVLIVELPGHGQSPAPDGAPTVEGASASLAKTLA
                     DRKVEHAILVGHSYGALVALKTAADQPKRARAVVAVDAATYTPADSVRIAEADRVLRE
                     RYTVFLSAVYQAMTKNPATGDSLLARAMRVEPSVLTEYFHDSWREDLRPAIRSLKTPV
                     HLVATEGLWPSAESWTSARKRLGYETAGPAEGHRVAESAHMVALDQPDSLAAIIEAVA
                     AHP"
     gene            complement(8313..9128)
                     /locus_tag="VFM00_12900"
     CDS             complement(8313..9128)
                     /locus_tag="VFM00_12900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7499190.1"
                     /translation="MSPAEDVRASTAEQAGPASSLAYRTPEIYDVATGWKLDLEIEFL
                     ERVFSRHAGVVKRVLEPCCGTARLLRALAEQGYGVAGYDLSPAMTAFARERLRPVGGE
                     VWLGEMSAFQPPHGFDAAINLVNSIGYLLEDDAVAGHLERIGSALRPGGIYVAQFSYG
                     GEPPELARFGPWGNRGGDLSTTLLWEVLREDEGARRSYQHCRITARRGKERHVIDEEH
                     VLRYWTHEDVTRFVARSPFALDAVYWDNFEEFPLEDYRVGEHGNLYHVLRRKD"
     gene            complement(9222..9422)
                     /locus_tag="VFM00_12905"
     CDS             complement(9222..9422)
                     /locus_tag="VFM00_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7499191.1"
                     /translation="MATGTVKWFNEAKGFGFIQQEGGEDVFVHYSAIVGEGFKTLAEG
                     ERLEFDIVRGPKGLQAANVRKA"
     gene            9784..10794
                     /locus_tag="VFM00_12910"
     CDS             9784..10794
                     /locus_tag="VFM00_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499192.1"
                     /translation="MEPSIDRDPAARLLDTPVRDLKLSLAGSPVERLTKRLVRELRAR
                     GLKRFTPEFYLTDEWGCPSGEPVIGVPFYLADRALMRFERRMNDLESPREIMMYLIHE
                     AGHAFNYAYRLYPTPEWRATFGPFRRRYRDDYRPVPFSRSFVRYLPGWYAQKHPDEDF
                     AETFAVWLTQSPGWRKRYEGWPALAKLEYMERVAAELGDKDPVVPKGRTDVTVDEMEQ
                     TVREVFQEMSARNRAALELKPDDDLADIFMKKDPKRKNTRAAWQIVAEHRLALVDAIA
                     YWTGVRRPVIRTMVDGTIEALRAAEYQGIRGREAEYLMRLTAYGTTLAFSYFSRGKFH
                     NL"
     gene            10806..11867
                     /locus_tag="VFM00_12915"
     CDS             10806..11867
                     /locus_tag="VFM00_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7499193.1"
                     /translation="MAEAPLRVAVLYDEWHEEKAPEPEEPAEPPTPGKRKKRREKLDR
                     EEVFEALKKRGHLPKYHCLDGRPRSLKALANLDVDVIFNLSESYAGDDTQDIAIAAYL
                     DLLGKAYTGSGPAGLHLAQDKAVAKRLFAFHGIRTPNFAAVYQGRLDWSHDLHFPVIV
                     KPKREDGSIGIEFNAVVGSIKELMERIDQLHADLDSPVLIEEYIEGRELYVGVLGNDQ
                     PQALPLVELNLSDLPEGTPRIAGTEVKWERGTAAYKKTKLRLPDDIPEELVAEIQDTA
                     CKACRALEVRDYARVDMRLKPDGTWYTLEVNPNPWLHSTAEFVLAARQSGRDQAGLIE
                     EIALMALARHRRRRARRSH"
     gene            complement(11800..13482)
                     /locus_tag="VFM00_12920"
     CDS             complement(11800..13482)
                     /locus_tag="VFM00_12920"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HET7499194.1"
                     /translation="MPDGGTTGCSFRFSFRGRRGMALGLTWTLRRWLSRCSRILPGVS
                     RAGAAGLLLLLPACAPAPEPGPGRPASARSRVVAENSRPGNRDWELSNPATGREIEGY
                     ASAASVRAGDSIAFHVSARSGRFDTRIYRLGWYGGAGARLVSSIRGTPGMARDLPEPR
                     QQDGLVACRWPASLRIGTGTDWQSGVYLARLTDAGTGKQAYVPFVVRELLGSTAPYLL
                     LLNTTTWQAYNDWGGTSLYDFNSRRRARALRVSYDRPYGSGPGAWRGLGAGELLTTPH
                     TARKAGWEYPMIRWLERNGIETAYAADLDLADSTLIAGRRGLLIAGHPEYWSRLMRDA
                     LERARDAGTGLALFGANAGYWQIRVEPSADGAAGRVLFCSKDYTRDSLFDTAADRDLT
                     VRFRNLHPRRPEVALLGVMTARGEESVEADFVPIPAMRNSWIYRGTGVAKGTTRALAG
                     LVGYETDRSFLGDSLYGKWSPAGLEVLSCSPIRFKDGSTENSEAVSYRAPSGAVVFSI
                     GSVQWSWGLDDWGSPALRPKRRHQDAERITLNVIGALGAGDAAPAPSARSPR"
     gene            13452..16298
                     /locus_tag="VFM00_12925"
     CDS             13452..16298
                     /locus_tag="VFM00_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7499195.1"
                     /translation="MNILSYRRPASRLPRRATTLASLLALAALVASAPAAFASVKPPQ
                     IKLDYEKYTLPNGLEVVLREDHRLPIVAVNTWYHVGPANEAAGRTGFAHLFEHMMFQS
                     SGHVGEDQFWKYMEGAGASFINGTTDFDRTNYMEDVPSNQLEMALWLESDRMGFLQDR
                     LTAASLANQQDVVRNERRQSVENAPYQIVEEEMWHMMFPKGHPYYAWVIGSHEDIQAA
                     KIDDVKAFFKQYYTPNNASLVIVGDIDKVKTKKLVEKYYGTIPRGPAVPAITATTPPL
                     TSEKRATVTDKVELPRVYFSWFTPPIFKAGDADAQIAADILGGGKASRMYKTLVYEKQ
                     IAQDVSVSQSSYQLGSVFTVQITVKPGHTPEEAEKAAQEVLDAFKAEGPTQAEIDAAQ
                     NSTYSGIVTSLENLGGFDGIANRMNQYNQFTKNPGYLNQDLARFAAVTPATAKKFAQD
                     YLKNDARVVIYGVPGDKKLPPNPTAPPKPTGDEAKIESGEPWRNTVPTPGPVSRAALP
                     TPKRFALPNGLQVYLVESHNLPVVAANLVLRSGSAADPKDTPGLAGFTASMLDEGTES
                     MDALAIANRMYGLGASLSTGSSADASNASVRSLKQNARAAMDVLGDVVLRPAFPAKEV
                     DRIRNDRLTSLLQQRDQPFQTAIRVMNASLYGPTHPYGHTALGTEESLKKISRDDLVS
                     FYKGAYGPKNAALVMVGDVTEAEAKKLATEVFGSWKGDGGAAPLPPEGATATSRVIIV
                     DKPESPQTAVLAGQVGVARSNPDYEKLDVMNTVLGGMFSSRINLNLREDKGYSYGAFS
                     FIGQNRGVGPVMAGAAVRADATGPSIDEILKEVARMRDAGVTDDELKMAKDSMVRSLP
                     ANFETTFSTAGTLAQIYMYDLPLDYYQALPGRVEAMTKDDVGAVAKKYLTPDRMLIVA
                     VGDKKVIEPQISKLSLGTIAYRDADGKEIVVSNQ"
     gene            16631..16840
                     /locus_tag="VFM00_12930"
     CDS             16631..16840
                     /locus_tag="VFM00_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499196.1"
                     /translation="MSTPRTRSETPVRSASSSSESSAVLGPAGLLTADSRDDAELHRA
                     IESAASRLLGLALARGVEEQDPDEE"
     gene            16857..17396
                     /locus_tag="VFM00_12935"
     CDS             16857..17396
                     /locus_tag="VFM00_12935"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7499197.1"
                     /translation="MTNETQTIDGAIEQVERLYESVTGREAPTLEDKPYATIPPEKVP
                     EEHVQEQVDRLIQTLAHFSGKPETEPEWKPAIALWEGRNEVRLVVDLPGVERDQVRVA
                     VSRGMLELTGIRVLRPTEDDSLKLRYAEHPYGKFRRTMPLPHGAKVEQLKAEMKAGVL
                     ELRIPRETEIVEIKTVTVG"
     gene            17502..18014
                     /locus_tag="VFM00_12940"
     CDS             17502..18014
                     /locus_tag="VFM00_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499198.1"
                     /translation="MEAKVDVRKLQILNDRINQTIDALSQVRLSVHGLGHTGVPTPMF
                     NPFSAYTQGLGVPTIPNFPGTPSFPPVSPYGMIPPIPGPIGLSHTPFNPLVNPLFNLP
                     TPYPTPFVNPMIPPVPPPGITPNWAPTPWTGFGGGLFHTPIDPIELKLLEMRAFDPIR
                     IRETFPFVGF"
     gene            complement(18121..20064)
                     /locus_tag="VFM00_12945"
     CDS             complement(18121..20064)
                     /locus_tag="VFM00_12945"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET7499199.1"
                     /translation="MELATIVMQDRLRYAKVLVEMRELQRAELEVAEILEETPEHLDA
                     LSLFTKIKHMRGQLSLAVACAAQLQSRRSISGEQGRMHLESMLHMAQDPVHGAGEFLA
                     LGQFQLVKKPTAYLALEEAFRQFVNRRPTEAEATCRRVAARHKETDPSVYKLAVLAEA
                     WIHELTGQLQPACEILERLGLERGFETDIDRLMALVALYEKIGTRPSLEAAVNICRYL
                     LNGADGTFVLGRLAMLHHRLGESEAAAEYEQRHLTAYRRMMHRAGFDDLLAVASRRFL
                     PIERLRQVHVPAMDAPPDASRRETAIAHAIRGDAAEARRGFSIAPEVLDLKYLADLEA
                     LENGNGRADRAVEGYVKVLRADPTDLNVIAWLLERQAASPSKAIAAVFQEPAVLAESL
                     QALERAVHDSPVDYRLWRQLSVLFGLMNRGADQQKQFADRAAALERTARERSQAIGRV
                     LSAATYRFAGTVHGLIHEIWATREMATPGRGGSLQRDDILGNLTPEMRDSVRNTFIAV
                     REYAQSMFPHLTRDIMDYHYGYKVTKEDEPSGGTSAGLPTALAFLSQFLQRPIRQDVA
                     LTGVLVTDAHDVLTVRTVGDIEQKVDAAYHRNLAMIVVPSGNRPILERSSLVPLTIQA
                     EIVRYASDLDEAVRIVLGEVELL"
     gene            complement(20111..20698)
                     /locus_tag="VFM00_12950"
     CDS             complement(20111..20698)
                     /locus_tag="VFM00_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HET7499200.1"
                     /translation="MPVRTDLVEAERRWRLPGETMLISQKMVAALNTQIGNEFGAFLQ
                     YVQIASHFGRENLPELSKHFYRQAEEEKVHALKIVNYVVDAGGSVAIPAIAAPRPAFA
                     NAQEAVQLSLDWEVTVTKQINGLVDLAIAENDHTSRNFLQWFVAEQLEEVSSMDMLLS
                     MVRRAGEQGLLFIEDFLARHKGMPVASPGPEGAGS"
     gene            21209..21448
                     /locus_tag="VFM00_12955"
     CDS             21209..21448
                     /locus_tag="VFM00_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499201.1"
                     /translation="MSPTRIPNPDLDDLPADLPTDDSARSVPEAERHVRLADRLNEAE
                     LEVIIQASALRLQNLVLVQASFEDDSEIAYAEEEA"
     gene            21445..21978
                     /locus_tag="VFM00_12960"
     CDS             21445..21978
                     /locus_tag="VFM00_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7499202.1"
                     /translation="MNETESIDTAIEQVERLYRSVTGQQPPVGEQPYATIPPEKVPEE
                     HVQEQVDRLVETLAAFSTGSRVETEWKPLITLWEAKEELVIAVDLPGTTRERVHVTVD
                     RGLLQVHGERPLRETKDGDGRTLKYVEHPYGRFRRVIPLPHAARVDQLQANLHDGVLE
                     IRIPKRESSESRTIPIA"
BASE COUNT         3572 a         7781 c         7583 g         3250 t
ORIGIN      
        1 acgtcgtcgg ccagcgaacg ctggccggcg accaggttcg cctgggcgcg atcggcctcg
       61 gcctgcagcc ggtcccgctc ccccgcgccg gcgcggaccc cgatggggac ggcaacggaa
      121 gcacccgcgc tccaccccgg aaaatctcta gcggtagcct cggaaatggc gtcgccgatc
      181 gttccttgcg gaacggtcgt cgtaactgtg tcggctccaa agatcacggt gcgggggtcg
      241 ccccgcagtc ccgtcccacc cagggtgccg aagacgtcca actgtggata cgcgttccaa
      301 cgggcaccgg tgtgtcgcgc tcgggcggca gccacgtcgc gctcacgcgc ccgcaactcc
      361 gggttggact ggagtgcccg cgtgacaagg tccccttcgg gctcgacggg gaagtctcgg
      421 ggtggctcat cggaaggatg aaaccgcgcg ggctcgctgg agggccgttc accgatctgc
      481 gaggctagcc ggtccgacgc ttggtccagc gcctcctgcc ggtcgaggac agcctgctcc
      541 tgttcggcca ggaagacttg cgcggtcgcg acctgacccg gacccacgag cccggcgcga
      601 ctccggagct gggcttggtt caggagtgcg gtcgcttgat cgcggatcag ttgcgcgacg
      661 gcaagatcgc gttcggccgc atacacctcc caataggtgc gttccacatt cgccctgacc
      721 aaggagacgg cgtcgtcata gcgcgcctgt gcggcttcct gctcccgatt cgtcgccgtc
      781 ctctcgctcc aggtgcccgg gccgaaaccc cgcagcaagg gctggcggag tgaaatgcgc
      841 cccgcggcat catagcgagg gcgcagggta ttgacggggg cgttgtcgtc cacgcggtcg
      901 gcgtcgagcg tggcggaaag ctgcgtaccg aacggaaggg tgatgcgagc gcctcccgtc
      961 aggaatgtct ggaggctctt cgttccggag aagattgagg tctgccgctg ctcgccttcc
     1021 agatggttca cgttcccaaa caactccgga tcgaacgccc cgcgctggcg gcgcgccgcg
     1081 ccccgcgccg cccggagcct cgcggcggcg atccgcacgt cggtcgagcc ctggagcgcc
     1141 gcgcccacgg cgtccgccag ggagagcggc tgtccctgga tttcggacag ggttcgggcc
     1201 agggcgctgt cgggattgga ggcgagcggc gagggagccg ggggcgattc gtccgacctc
     1261 gcggccggct gcgcgatcgt gggcgtgacg gatcgaaacg aaagaacgac ggccgcggtc
     1321 gcggccagga gccaagcgcc ggcaaacgcg caccggcgct gccgaggcga cgcctcggcg
     1381 ggcgctgccg atcctgtgat caaggagtcc cccaccccga ttgttcgggc ctttccggtc
     1441 cgatgacgaa gacggcgagt ttacgcttcc gcgggaacga cttccagcgt ttgaagctcg
     1501 accccccgga gcaccgacag gctgcgcgga accccggcgg cctggtcgat cagcttggcc
     1561 tggaggtcgt cgacgctggc caccgggtcc tccccgaagc cgaggatgac gtcgccctcc
     1621 ctggcgccgg cccgctcggc gggaccgttg ggctggaggc tctggatcag gaccccgctc
     1681 tccaccggga ggcggaagta gcgggccacc ttgcgcggca gcgtcacgtt ctgccccgcg
     1741 atcccgatca cgctccggcg gacccgcccg aactggatga gctggctcgc gacgcgcttg
     1801 gcggtgttga ccgcgatggc gaagcagatc ccctgggccg gaaggatgac ggcggagttc
     1861 acgccgacca cctctccctg ggcggtgacg agcggtccgc cggagttgcc tggattgagg
     1921 gccgcgtcgg tctggatcac gttgtcgatg agacgacccg atccggagcg cagcgagcgt
     1981 cccaatgcgc tcacgacgcc ggccgtgacg gtggcctgga atccgaacgg gtttccgacc
     2041 gcgatcacga gctgccccac caggagggac tgcgagtcgc ccagcgtcgc cggcgtgagg
     2101 ttgggggcgt gaatgcggat cacggcgagg tcggactccg gatcgtcgcc gatcagctgg
     2161 gcttcgtgcc gccctccctc gggaaggtgc accacgatcg acgaagcccc gtgcacgacg
     2221 tggctgttcg tgaggatgaa cccgtctggg gtgaacacga atccggaccc ggatccgccg
     2281 cgcggcatgg gacggcgccc ccgcgcctcg ggcccgggct cgggatggcg cacctcgatc
     2341 gaaacgaccg agggacccgc gaccgcggcg gcgtggatga ccgcacgcga ataggcgtcg
     2401 agcggcgccg ggtgctgccg tgcctcgaac tcggggagag gctccatctc gaaggaatcg
     2461 ccgctcggct cgcgctgtgc catggggctt tggatgctcc gcaacggggc gggtttccgg
     2521 cggaatcccg ccgcgcaccg gcgccgagac ttccaggcgc ctgttcgctt atgatgacgg
     2581 ggtgaccgca cgaatgtccg gcccgctgga acccacacgc agggctcacg ccgcgtgacc
     2641 ccatccacgg aattctatct cggcgatccg gaggaccacg gccggcttcg ctgcctggtc
     2701 tgggaagtgg agcgcccgcg gggccgcatc agcatcatcc acggcctggg ggaccatgcc
     2761 gcccgctata cccacgtggc cgaggcgctg gcgggccgcg gcttctcggt ggaggcgatg
     2821 gacctccccg ggcacggcaa gtcgggcggc ccgcgcggcc acgtccgcca ctgggacgac
     2881 taccgcggcg ccgtcgagac ctggatgcgg cactccgctt cccttcccgg ggcggagacc
     2941 cgcgcgatcc tgggccagag catgggcgcg ttcgtcgcgc tcgactggac cctgggccac
     3001 ccgcgcgagg tgcgggcgct ggtgctctgc ggcgcgccct tcgaggtcgt gctcaagccc
     3061 tcgatgctga aggtcaaggc cgcccagctg gccgcgcgca tctggcccgg gttttcgcaa
     3121 gggaacacca tcctcccctc gatgctctcg cgcgatccgg aggtcgtgcg ggcgcactcg
     3181 gtcgatcccc tggtgcacta caagatcagc gcgcggctct tcttcgaatt ccagcgcatg
     3241 cgcctggagt tgatgcgccg cgccgcggag ctgccggtgg acacgctggt catccacggg
     3301 ggcgccgacc cgatctcgag cgtggtcggc gcgcagcgct gggccgcgcg gtgcccccag
     3361 gggcgggtca cctgccggct ctatcccgga ctgctgcacg aggtgctgaa cgagctggag
     3421 cgccgcgagg tgatggagga cgtcgtggga tgggtcgagg gcgtgatgcc gggggccggg
     3481 gcggcctgat ccgctagtcc agcaggcgtt cgaccgccat cctccatgag gcgaggcgac
     3541 ggcggcgctc ggctccggcc atccgcggcc ggaacacccg ctctcccgag cgcgctgccg
     3601 gaacgtcggc gcgatccttc cagatccccg cgccgatccc cgcgagcagc gccgcgccca
     3661 aagccgtgct ctcgagcatc gccgggcgcg tgatcgggat gcccaggaga tcggcctggg
     3721 tctgcatcag gagatcgttc gcggcggcgc cgccgtccac ccgcagcgcg cgaacgcgcg
     3781 cccgcgcgtc cttctccatc gccgacacca cgtcgcacgt ctggaacgcg atcgattcga
     3841 gggcggcccg ggccagatgg ccccgcgtcg tgccacgcgt gaggcccgac cagacgccgc
     3901 gggcatcggg ccgccaccag ggcgcgccga gccccgcgaa cgcggggacg agcaccgtcc
     3961 cgcccgcgtc cggcaccgag cgcgcgagcg cctccacctc ggaggatgtc gcgatgatgc
     4021 cgagaccgtc gcgcatccac tgcaccgccg ccccggcgat gaagaccgac ccctcgagcg
     4081 cgtaggccgg ctcgccgcgc ggcccgcacg cggccgtcgt gaggagtccc gagcgcgagg
     4141 ccgtgggcgc cgcaccggtg tgcagcatga ggaagcaccc ggtgccgtac gtgttcttga
     4201 tgccgccccg ccgcacgcac ccctggccga agagcgcggc ctgctggtct ccggcgatcc
     4261 cccggatcgg aatcccgtcc ggaaggccgg gaacgccgcg ggtgcttccg aagtcgccgg
     4321 ccgagggacg cacttcggga agcaccacct cgggtacgcc gaagagcgcg gccagctccg
     4381 ggtcccaccg gcggcgccgg atgtcgtaga ggagcgtccg gctcgcgttg gtgggctccg
     4441 tggcgtgcac ggcgccgccg gtgagcttgg cgacgagcca cgagtccacg gtgccgaagg
     4501 cgatggcgcc gcggcgcgcc cgggcgcgga ggtcccggtc gtgcgccagg agccactcca
     4561 ccttggtcgc cgaaaaatag ggatcgaggc ggaggccggt ccgcttccgg acccaagact
     4621 cgtgccccgc gcggcggagc gcctcgcatc gctcggcggt gcggcgatcc tgccacacga
     4681 tcgcgggtgc ggcgggacgg agcgcgcgcc ggtcccagag catcgccgtc tcgcgctggt
     4741 tcgtgatgcc gatcgcggcg atgcgctccg cgcgggcgcc gcggagtgcc gcgcgaagcg
     4801 cgcgcagcgt cgtgtcccag atctcctcgc cgcgatgctc cacccatccc ggccgcggga
     4861 aatgctgcgg cagctcggcg tatccccgtg ccgtaacgcg ggcccggtcg tccagcagga
     4921 gcaccgtcgt gccggtggtg ccctggtcga tggcgagcag cagcttcacg cgatcccccg
     4981 cgttcccggc tccgcgtggc ccgagccgac cgccgcgtcg atggcccgct cctctcgatc
     5041 caggatcgcg agaaggcgct ccacctcggc cgaccgggtc cgctccgacc agcccagcac
     5101 cggcgcggcc cacaccgcgc agggcatcgc ggcttcgcga agagcccgcg cgtccagcca
     5161 gaggcgggtc cggcgcagca gaatatcgtc gaggcggcgg gccctctcca cgcgcgccgc
     5221 gtgggccact tccgcctccg agatgtggaa gccgcccgcg acctcggccg cgcggggaaa
     5281 gtcgcgccgg agcctctcga tcgattcggc gcgccccgaa cggagcggaa gcgaggcggt
     5341 gacgcagggg gccacgcgcg cgcccagcgc gcggacgatg ccgttcacga cgcgctcggc
     5401 gagcgcccgg tgcgtcgtgt acttccctcc gaccatcgtc cagagccctc gctccacgag
     5461 cacgcggctc tcgcggctgt tctcccaggg acgctcggcc cgtccacgcg acagggggcg
     5521 cactccggcg aaggcccgca cgggccgcac gccggcgagc gcgcccggaa gcgcggcggc
     5581 ggactcttcg aggaggtagc gcacgtcctc gacgtgcggc tccaccgacc cgggatcgga
     5641 gggatcgtcc acgtcggtgg tgccggccag cgtgtgcgct ccccaaggga tcacgaagaa
     5701 gacgcggccg tcccggcggg ctgtcaaaag aagcgccgtc ctgggaggcg ccgcggcgaa
     5761 caccacgtgc gcgccgcgcg tgcggcgcac gctgggcgcg ggatgccctt cggccatcgc
     5821 gcgcacgtcg tcgacccacg gtccgccggc gttcaccacc gcgcgcgctt ccacctcggc
     5881 gtcggtcccc tcgatccggt cgtggaagtg cgcgcgccag gctcgcccgt ctgcggtctt
     5941 ggcgagcgcg atcgcttcgg tgtgggtccg gaccgtagca cccgcccgtt cggcgtccac
     6001 ggccagcgcg acacagagcg ccgcgtcgtc catctgcgcg tcggcgaaga gcgacccgcc
     6061 gcggagatcc tccggatcga gcccgggcgc gagccggagg gcctcctctg ccccgatccc
     6121 gctccgctcc gcggcggtgc cgcccgcgag gaggcgatag agcgcgagac cggcgcccac
     6181 ccaccagcgc gcgcggccgc ggccggcgta atgcggcaac aggaacggaa gaggccggac
     6241 gaactcgggc gcggtgtgga gcaggatggc gcgctcgcga agcgcctcgc gcacgaggcg
     6301 gatctggccg ttttcgaggt agcgaaggcc gccgtggatc agcttcgagg tgcggctgct
     6361 ggtcgccgag gcgagatcgc ccttctcgag gaggagcgtc tccagtccgc gccgcgccgc
     6421 atcgcgcgcg acggcgacgc cgtggattcc tccgccgatc acgaggatgt cgaccactcg
     6481 attcctcccc ccacgcgtcc ctgcgcggcc ggccgcagtg tagtggaggg cgcggaaggc
     6541 cgccagatgc gatccgtgct aggctctgcc cgcttcgatc atcgcacgtc cgccgcgccg
     6601 gcgcgccacc cctggaggaa tcgcatggcc cccctcgctc cccttgaagg acgccgcgcg
     6661 ctcgtgaccg gagcgaccag cggcttcggc ctcgccatcg cgcgccgctt cgcgtcggag
     6721 ggcgcgcgcg tcgtcgtcac cggccggcgg gaggaccgcc tgcgcgcggt cgccgacgag
     6781 ctgcgcgcga aaggcgcgga cgcgacggcg ctggtcttcg acgtgcggga ccgcgctgcc
     6841 gtcgagcgtg cgctctccga ccgcgccctc gtggagagcc tggacatcgt ggtgaacaac
     6901 gcggggctcg cggcggggct cgatccggtt cagacggccg atcccgagga ctgggagcgg
     6961 atgatcgaca ccaacgtgaa ggggctcctc tgggtcacgc gcgccgtgct tccggcgatg
     7021 atcgccaaga cgcgcggcga cgtcgtgaac atcgggtcgg tcgctgggca ccaagcctac
     7081 ggcggcggct ccgtctacgc cgccaccaag ttcgccgtgc gcgccatcac ggaggcgctg
     7141 cgctacgacg tgctcggcac cggcgtgcgc gtgatgaacg tggagccggg cctggcggag
     7201 accgagttca gcctcgtgcg ctttcacggc gaccaggcgc gcgccaaggc ggtctaccgc
     7261 ggcgtgcggc cgctgacggc cgaggacgtg gccgacgcgg tcgcgtgggc ggtcacccgc
     7321 cccgcgcacg tcaacgtgca ggagatcctc ctcatgccca ccgatcaggc ctcggcctac
     7381 gccgtgcacc ggcgcgagga gccggcgcgc tgaaccgaag tcccgccccg gtgtatactt
     7441 ttccgatgcc catcccatcg cgaacccgtt tcggcgcggc caccgcggcg ctcgcgctcg
     7501 tcctggccgc ttcggcgctc gccggaaaac ccgcgccgct cgcggtccag gagtcgggct
     7561 cgggctcccc tacgatcgtc ctcgtgcaca gcatcggcgg ggaccggaac gactggagcg
     7621 ccgtggcgcc ccgcctggcg gagcatcacc gcgtgctgat cgtggagctt ccgggccacg
     7681 gtcaaagccc tgctcccgac ggggccccga cggtggaagg ggcgtcggcc tcgctcgcga
     7741 agacgctcgc cgatcgcaag gtcgagcacg cgatcctggt cgggcattcc tacggcgcgc
     7801 tcgtcgcgct gaagaccgcg gcggatcagc cgaagcgggc ccgcgccgtg gtcgcggtgg
     7861 acgccgccac ctacacgccc gccgactcgg tgcggatcgc cgaggccgac cgggtgctgc
     7921 gggagcggta cacggtcttc ctgagcgcgg tctaccaggc gatgacgaag aatccggcga
     7981 cgggggacag cctgctcgcg cgggcgatgc gcgtggaacc ctcggtgctg accgagtact
     8041 tccacgattc ctggcgtgag gatctgcggc ccgcgatccg gtcgctgaag accccggtcc
     8101 acctggtcgc gaccgagggg ctatggccct ccgcggagtc gtggaccagc gcgcgcaaac
     8161 gattgggata cgagacggcc ggacccgccg aggggcatcg cgtggcggaa tcggcgcaca
     8221 tggtggccct ggatcagccc gattcgctgg ccgccatcat cgaggccgtc gccgcgcacc
     8281 cctgaccgcg cggggcgcgc cccgcgaccg cgtcagtcct tcctgcggag cacgtggtac
     8341 aggttcccgt gctccccgac ccggtagtcc tccagcggaa actcctcgaa gttgtcccag
     8401 tagaccgcgt ccagcgcgaa ggggcttctg gccacgaacc gggtcacgtc ctcgtgggtc
     8461 cagtagcgca gcacgtgctc ctcgtcgatc acgtggcgct ccttgccgcg gcgcgccgtg
     8521 atccggcagt gctgatagga gcggcgcgcg ccctcgtctt cgcgcagcac ctcccagaga
     8581 agcgtggtcg agaggtcgcc tccccggttt ccccaggggc cgaagcgcgc cagctcgggc
     8641 ggctcgccgc cgtaggaaaa ttgcgcgacg tagatacctc cggggcgcag cgcgctcccg
     8701 atacgctcca gatggccggc gacggcgtcg tcctcgagca ggtagccgat ggagttgacc
     8761 aggttgatcg ccgcgtcgaa gccgtgcggc ggctggaagg cgctcatctc acccagccac
     8821 acctcgccgc ccaccgggcg aagccgctcg cgggcgaacg cggtcatcgc ggggctcagg
     8881 tcgtatcccg cgacgccgta gccctgttcg gcgagggcgc gcagaaggcg cgcggtgccg
     8941 cagcagggct cgaggacgcg cttcacgacg ccggcgtgcc ggctgaagac gcgctccagg
     9001 aattcgattt ccagatcgag cttccagccg gtcgcgacgt cgtagatctc cggggtgcgg
     9061 taggcgagcg acgaggcggg accggcctgt tcggcggtgg acgcgcggac gtcttcagcg
     9121 gggctcaagg gggaatcccc tccgaacagg gggaaatgac agccggggcg agcctttcgg
     9181 cccgccccgg gacatgacac gcagagtggt tcgctcggcg gctatgcctt gcggacgttc
     9241 gccgcctgca gacccttcgg tccgcggacg atgtcgaact ccagccgctc gccttcggcc
     9301 agcgtcttga agccctcgcc cacaatcgcc gagtagtgaa cgaagacgtc ctcgccaccc
     9361 tcctgctgga tgaacccaaa gcccttcgct tcgttgaacc acttcacggt tccagttgcc
     9421 acggacagaa ctcctcctac tgacggggcg tagagcccct tccaacgacc gccccgaagg
     9481 gcggcacgcc cggccacttt ggtcgggctt ccggcgcggg gcccggttgg gggcccggat
     9541 gcgccggata caagaaaagc cgcaggcgaa ctctcctgcg gcgtgaagac acgttcggtg
     9601 tttcgtacaa ccgtcaggca acaaaaggag tatcgcccgt tccggcccgt ctgtaaagcg
     9661 gaaagtgggg atggcgcagg ggcgcgggcc ggaacgggtt acgccgggac cctcgtagct
     9721 ggtacactcg cgcgcccaga accgccgcgg cggccccgcc ccgcggtccg agggcgaccg
     9781 accatggaac catccatcga ccgcgacccc gcggcccggc tcctggacac gcccgtccgg
     9841 gatctcaagc tctccctggc gggctctccg gtggagcgcc tcacgaagcg gctcgtgcgc
     9901 gagctgcgcg cccgcggact caagcgcttc actccggagt tctacctcac cgacgagtgg
     9961 ggctgcccct caggcgagcc ggtcatcggc gttcctttct atctcgccga tcgcgcgctg
    10021 atgcgattcg agcggcgcat gaacgatctg gagagccccc gcgagatcat gatgtacctg
    10081 atccacgaag cgggacacgc gttcaactac gcgtatcgcc tctacccgac gccggagtgg
    10141 cgtgctacgt tcggcccatt ccgccgccgc tatcgggacg attaccggcc ggtgccgttc
    10201 agccggtcgt tcgtgcgcta tctcccgggc tggtacgcgc agaagcatcc cgacgaggat
    10261 tttgccgaaa cgttcgcggt gtggctgacg cagtcgcccg ggtggaggaa gcgctacgag
    10321 ggatggccgg cgctcgccaa gctcgagtac atggagcgcg tggcggccga gctgggcgac
    10381 aaggacccgg tcgtcccgaa gggccggacc gacgtcaccg tggatgagat ggaacagacg
    10441 gtgcgcgagg tcttccagga gatgtccgcg cgcaatcgcg cggcgctcga gctgaagccg
    10501 gacgacgatc tggccgatat cttcatgaag aaggatccca agcgcaagaa cacgcgcgcg
    10561 gcctggcaga tcgtcgccga gcaccggctg gccctggtgg acgccatcgc gtactggacc
    10621 ggcgtgcgcc gcccggtcat ccggaccatg gtggacggca ccatcgaggc gctccgcgcg
    10681 gccgagtacc aggggatccg cggccgggag gccgaatatc tcatgcgact gacggcgtac
    10741 ggcaccaccc tcgcgttcag ctacttctcg cgaggcaagt tccacaacct ctaggagagg
    10801 agcgcatggc cgaagccccg ttgcgcgtgg cggtcctcta cgacgagtgg cacgaagaga
    10861 aggcgccgga gcccgaggag ccggccgagc cgccgacgcc cggaaagcgg aagaagcgac
    10921 gcgagaagct ggaccgggaa gaggtgttcg aggcgctgaa gaagcgcggc catctgccca
    10981 agtatcactg tctcgacggc cgcccgcggt cgctcaaggc gctcgccaac ctcgacgtcg
    11041 acgtcatctt caacctgagc gagtcgtacg cgggcgacga cacgcaggac atcgccatcg
    11101 ccgcctatct cgacctgctg gggaaggcgt acaccggctc cggtccggcg ggactccacc
    11161 tcgcccagga caaggccgta gccaagcggc tcttcgcctt ccacggcatc cggacgccga
    11221 acttcgcggc cgtctaccag gggcgcctgg actggtcgca cgacctccac ttccccgtga
    11281 tcgtgaagcc caagcgcgag gacggctcga tcggcatcga gttcaacgcc gtcgtcgggt
    11341 cgatcaagga gctgatggag cgcatcgatc agctccacgc cgatctcgac tcgccggtgc
    11401 tcatcgagga atacatcgag gggcgcgagc tgtacgtcgg cgttctgggg aacgaccagc
    11461 cccaggccct tccgctggtc gagctgaacc tgtccgatct tccggaaggg acgccgcgca
    11521 tcgccgggac cgaggtcaag tgggagcgcg ggacagccgc ctacaagaag acgaagctcc
    11581 gcctgcccga cgacatcccc gaggaattgg tggccgagat ccaggacacg gcgtgcaagg
    11641 cctgccgcgc gctcgaagtg cgcgactacg cgcgcgtcga catgcggctc aagcccgacg
    11701 gcacgtggta cacgctggag gtgaacccca acccctggct ccactccacc gccgagttcg
    11761 tcctcgcggc ccggcagtcg gggcgcgacc aggccgggct catcgaggag atcgcgctga
    11821 tggcgctggc gcggcatcgc cggcgccgag cgcgccgatc acattgagcg tgatccgctc
    11881 cgcgtcctga tgccggcgct tcggacgaag cgccggcgag ccccagtcgt ccagccccca
    11941 cgaccactgc accgatccta tcgagaagac gaccgcccct gagggggccc ggtagctcac
    12001 cgcttccgaa ttctcggtgc tgccgtcctt gaagcggatg ggcgaacacg acagcacttc
    12061 gagtccggcc ggcgaccact tgccatagag cgaatcgccc aggaacgagc gatcggtctc
    12121 gtacccgacc agccccgcca gcgcgcgcgt cgttcccttg gcgacgcccg ttccccggta
    12181 gatccacgag ttccgcatcg cgggtatcgg cacgaaatcg gcctcgacgc tctcttctcc
    12241 gcgcgccgtc atgacgccga ggagcgcgac ctcgggccgg cgcggatgca ggttccggaa
    12301 gcggaccgtc aggtcccggt cggccgcggt gtcgaagagc gaatcgcgcg tgtagtcctt
    12361 ggagcagaag aggacgcgac cggccgcgcc gtcggccgag ggctcaacgc ggatctgcca
    12421 gtagcccgcg ttcgccccga acagggcgag tccggtcccg gcgtcgcgcg cccgttccag
    12481 cgcgtcgcgc atgagccgcg accagtactc gggatggccc gcgatcagga gcccccgccg
    12541 gccggcgatc aaggtggaat cggcgagatc gagatcggcc gcgtaggcgg tctcgatccc
    12601 gtttcgctcg agccagcgga tcatggggta ctcccatccc gctttccgcg cggtgtgcgg
    12661 cgtcgtgagg agctcccccg cgcccagacc ccgccatgcg cccggacccg acccgtaggg
    12721 gcggtcgtag gagacccgga gggcgcgggc ccgccggcgg ctgttgaagt cgtagagact
    12781 ggtcccgccc cagtcgttgt aggcctgcca ggtggtcgtg ttgaggagaa gaaggtaggg
    12841 ggcggtcgat cccaggagct cgcgcaccac gaacggaacg tacgcctgct tcccggtccc
    12901 ggcgtcggtc aggcgggcca ggtagacccc gctctgccag tcggttccgg tcccgatccg
    12961 gagcgacgcc ggccaccggc aggcgaccag cccgtcctgc tgccggggct cgggaaggtc
    13021 ccgcgccatt ccgggggtcc cccggatact cgatacgaga cgcgcgcccg cgcccccgta
    13081 ccaacccaaa cggtagatcc gggtatcaaa acggccggaa cgtgcgctga cgtggaaggc
    13141 gatggaatcg ccggcccgga cactcgccgc ggaggcatag ccttcgatct cccggcccgt
    13201 ggcagggttg gaaagctccc agtcgcggtt tcccggcctc gagttctcgg cgacgacccg
    13261 gctccgcgcg gaagcgggtc tcccgggccc gggttcggga gccggggcgc atgccgggag
    13321 cagcagcaga agcccggcgg cccctgccct tgacactccg ggaaggattc gggaacatcg
    13381 cgaaagccag cgacgtagcg tccacgtcag ccccaaggcc attccccgcc gccccctgaa
    13441 ggagaaccgg aatgaacatc ctgtcgtacc gccgtccggc atcgcgattg ccgcgccgcg
    13501 cgaccacgct cgcctcgctt ctcgcgctcg ccgctctcgt cgcgtcggcg cccgcggcgt
    13561 tcgcgagcgt caagccgccg cagatcaagc tggattacga gaagtacacc cttccgaacg
    13621 gtctcgaggt ggtcctccgc gaggaccatc gccttccgat cgtcgccgtc aacacctggt
    13681 accacgtcgg tccggccaac gaagcggccg gccgcaccgg cttcgcccac ctcttcgagc
    13741 acatgatgtt ccagtcctcg ggccacgtgg gtgaggacca gttctggaag tacatggagg
    13801 gcgcgggcgc ctcgttcatc aacgggacca ccgacttcga tcgcacgaac tacatggagg
    13861 acgtcccctc caaccagctc gagatggcgc tgtggctgga gagcgaccgc atgggcttcc
    13921 tccaggaccg gctcacggcg gcctcgctgg cgaaccagca ggacgtcgtg cgcaatgagc
    13981 ggcggcagag cgtcgagaac gccccgtacc agatcgtcga ggaagagatg tggcacatga
    14041 tgttccccaa gggccatccc tactacgcgt gggtgatcgg ctcgcacgag gacatccagg
    14101 ccgccaagat cgacgatgtg aaggcgttct tcaagcagta ctacacgccg aacaacgcct
    14161 ccctcgtcat cgtcggcgat atcgacaagg tgaagacgaa gaagctggtc gagaagtact
    14221 acggcacgat cccgcgcggc ccggccgtcc cggccatcac cgcgacgacg ccgccgctca
    14281 cgtcggagaa gcgcgcgacc gtgacggaca aggtcgagct gccgcgggtc tacttctcct
    14341 ggttcacgcc gcccatcttc aaggcgggcg acgccgacgc gcagatcgcg gccgacatcc
    14401 tcggcggcgg caaggcgagc cggatgtaca agacgctcgt gtacgagaag cagatcgcgc
    14461 aggacgtgag cgtctcccag agttcctatc agctcggatc ggtcttcacc gtgcagatca
    14521 cggtcaagcc gggacacacc cccgaagagg cggagaaggc ggcgcaggaa gtcctcgacg
    14581 cgttcaaggc cgagggaccg acgcaggccg agatcgacgc cgctcagaat tcgacctact
    14641 ccgggatcgt caccagcctc gaaaacctgg gcggtttcga tggcatcgcg aaccggatga
    14701 accagtacaa ccagttcacg aagaatccgg gctacctgaa ccaggatctg gcccgcttcg
    14761 cggcggtcac ccccgccacg gcgaagaagt tcgcgcagga ctatctcaag aacgacgcgc
    14821 gggtggtgat ctacggggtc cccggcgaca agaagctccc gccgaatccc acggcgccgc
    14881 ccaagccgac gggcgatgag gccaagatcg aatccggcga gccgtggcgc aacacagtgc
    14941 cgaccccggg cccggtctcc cgggccgcgc tcccgacgcc gaagcgcttc gcccttccga
    15001 acggactcca ggtctacctg gtcgagtccc acaaccttcc ggtcgtggcc gcgaacctcg
    15061 tgctccgttc cggatccgcc gcggatccca aggacacccc gggtctcgcc ggcttcaccg
    15121 cctcgatgct cgacgaggga accgagtcga tggacgcgct ggcgatcgcc aaccggatgt
    15181 acgggctggg ggcctccctg agcaccggat cctccgccga cgcgtcgaat gccagcgtcc
    15241 gttcgctcaa gcagaacgct cgcgcggcca tggacgtgct gggcgacgtg gtcctccggc
    15301 ccgcgttccc ggccaaggaa gtggaccgga tccggaacga ccgcctcacc tccctgctcc
    15361 agcagcgcga ccagccgttc cagaccgcga tccgggtcat gaacgcctcc ctctacggtc
    15421 ccacgcatcc ctacggccac accgcgctcg ggaccgagga gtcgctcaag aagatttccc
    15481 gcgacgacct cgtctcgttc tacaagggcg cctacggccc gaagaacgcc gccctcgtca
    15541 tggtcggcga cgtcacggaa gccgaggcca agaagctcgc gaccgaggtt ttcggctcct
    15601 ggaagggtga cggcggtgcc gcgccgctcc cgcccgaggg cgcgacggcg acctcgcggg
    15661 tcatcatcgt ggacaagccg gaatcgccgc agaccgcggt gctcgccggc caggtcggcg
    15721 tggcccgctc gaatcccgat tacgagaagc tggacgtgat gaacacggtg ctcggcggga
    15781 tgttctcgag tcggatcaac ctgaacctgc gcgaggacaa gggctattcg tacggcgcct
    15841 tctccttcat cggccagaat cgcggcgtgg gccccgtcat ggcgggtgcg gccgttcgcg
    15901 ccgacgccac gggcccgtcg atcgacgaga tcctcaagga agtcgcgcgg atgcgagacg
    15961 cgggcgtcac ggacgacgag ctcaagatgg cgaaggactc gatggtccgc tcgctcccgg
    16021 ccaacttcga aacgaccttc agcacggcgg gcacgctggc gcagatctac atgtacgatc
    16081 tgccgctgga ctactaccag gcgctccccg gccgcgtgga agcgatgacg aaggacgacg
    16141 tgggcgcggt ggcgaagaag tacctgacac ccgaccgcat gctcatcgtg gcggtgggtg
    16201 acaaaaaagt catcgagccg cagatctcga agctctcgct cggcaccatc gcctaccgcg
    16261 atgcggacgg gaaggagatt gttgtaagta atcagtgatc ccgcttcgcc gcagcgaagc
    16321 ggaaccccag ttctttagcg caggggccgc ccttcggggc ggcccctgcg tgtgtcaggt
    16381 ccgtcagcca tgacatgaca aaacggcggt gccttcccca gttccttctt ccgctcctcc
    16441 tcaacgcagc tgcgccagat cggcgcaccc cctcagatca cgcgtaccga cgcgcctcgt
    16501 gcgcacgccg ctactacctc tgcggcgcgc ttcgcgcgcc gcaggacata ggcacacctc
    16561 atgcagcctc accggccgca ccgcaatcac cccaattgaa cgacgcagcc cttcgaacga
    16621 aggagatctc atgagcacgc cgcgcacacg gtcggaaacg ccggtccgat ccgcttcgag
    16681 ctcaagcgaa tcctcggccg tcctcggacc ggcggggctc ctgaccgccg attcccgcga
    16741 tgacgccgag ctgcaccggg ccatcgagtc cgccgcgtcg cggctcctgg gccttgctct
    16801 ggcgcgaggc gtcgaggagc aggaccctga cgaagagtga agcgaaggag gtactcatga
    16861 cgaacgagac ccagaccatc gatggcgcca tcgagcaggt ggagcggctc tatgagtcgg
    16921 tcaccgggcg cgaagcgccc acgctggaag acaagcccta cgcgaccatt ccgcccgaaa
    16981 aggtccccga ggagcacgtt caggaacagg tggaccggct gatccagacg ctcgcgcatt
    17041 tctcgggcaa gcccgagacc gagccggaat ggaagccggc gatcgcgctg tgggagggtc
    17101 gcaacgaagt tcggctcgtg gtcgatctcc cgggtgtgga gcgggaccag gtgcgcgtgg
    17161 cggtcagccg cggcatgctc gagctcaccg ggatccgggt gttgcgcccc accgaggacg
    17221 attcgctcaa gctccggtac gcggagcacc cgtacggcaa gttccgccgg acgatgcccc
    17281 tgccccatgg cgccaaggtc gagcagctga aggccgagat gaaagccggg gtcctggagc
    17341 ttcgcattcc gagagaaacc gagatcgtcg agatcaagac cgtgaccgtc ggctgaacgg
    17401 agcaggtcat gacccgtcat cccaggaaac gaacgccacc gccacggaag gccgccgtcc
    17461 cgaaacgacc ggggcacgac ggcacacgca aggaggagtc catggaagca aaggtcgatg
    17521 tccggaagct ccagattctc aatgaccgga tcaatcagac gatcgacgcc ctgagccagg
    17581 tccgtctctc ggtccatggc ctcgggcaca cgggggttcc tacgcccatg ttcaacccgt
    17641 tcagcgccta cacccaggga ctgggagtgc cgacgattcc gaacttcccg ggcaccccgt
    17701 cgttcccgcc ggtttcgccg tacggcatga tcccgccgat tccggggccg atcgggcttt
    17761 cgcacacgcc gttcaacccg ctcgtgaacc cgctgttcaa cctgccgacc ccgtacccca
    17821 ccccgttcgt caacccgatg atcccgccgg ttccgccgcc gggcatcacg ccgaactggg
    17881 ctccgacgcc atggacgggc ttcggcggcg ggctgttcca cacgccgatc gatcccatcg
    17941 agctgaagct tctggagatg agggccttcg atccgatccg gatccgggag accttcccct
    18001 tcgtcggatt ctaggagggg cgtaacaacg taaggccagg ccgggccggt gcggccaagg
    18061 gggcgggcgc tcagcgtccg cccccgtttc atgggaagcg gcgcgccggc ggcggacgga
    18121 ctagagcagc tcgacctcgc cgagcacgat ccgaaccgcc tcgtcgaggt cggaggcgta
    18181 gcgaacgatc tcggcctgga tcgtgagcgg aacgaggctg ctccgctcga ggatcggccg
    18241 gtttccgctg ggcacgacga tcatcgcgag gttgcgatgg taggccgcgt ccactttctg
    18301 ctcgatgtcc cccaccgtgc gcacggtgag cacgtcgtgc gcgtcggtga ccaggacccc
    18361 ggtcagggcg acgtcctgcc ggatggggcg ttgcaggaat tgagagagga acgcgagcgc
    18421 cgtcggaagc ccggcggagg tgcctcccga gggctcgtcc tccttcgtca ccttgtagcc
    18481 gtagtggtag tccatgatgt cgcgcgtcag gtgagggaac atcgactgcg cgtactcccg
    18541 gaccgcgatg aacgtgttcc gcacgctgtc ccgcatctcg ggcgtcaggt tgcccaggat
    18601 gtcgtcccgc tgcagcgagc cgccacgccc cggcgtcgcc atctcccgcg tggcccagat
    18661 ctcgtggatc aggccgtgca ccgtgccggc gaaacgatac gtcgccgccg agagcacccg
    18721 cccgatcgcc tggctccgct cgcgcgcggt gcgctccagc gccgccgcgc gatccgcgaa
    18781 ctgcttctgc tggtcggcgc cgcggttcat gagcccgaag agcacggaga gctgtctcca
    18841 gagccggtag tccacggggc tgtcgtgcac cgcgcgctcc agcgcctgga gtgattccgc
    18901 gaggacggcg ggctcctgga acaccgcggc gatggctttc gagggcgagg ccgcctgccg
    18961 ctcgaggagc cacgcgatca cgttcaggtc cgtggggtcg gcgcgcagta ccttgacgta
    19021 gccctccacc gcgcggtcgg cgcggccgtt cccgttctcc agcgcctcga gatcggcgag
    19081 gtacttgaga tcgagcacct ccggggcgat cgagaagccg cgccgcgcct cggccgcgtc
    19141 gccgcggatg gcgtgagcga tcgcggtctc gcgccgtgag gcgtccggcg gcgcgtccat
    19201 ggcgggcacg tgcacctggc ggagccgctc gatcgggagg aagcggcgcg aggccacggc
    19261 cagcagatcg tcgaacccgg cccgatgcat catccggcga taggcggtca gatggcgctg
    19321 ctcgtattcg gcggccgctt ccgattcgcc gagacggtga tggagcatgg cgaggcggcc
    19381 gaggacgaag gtcccgtcgg ccccgttcag gaggtaccgg cagatgttca ccgccgcctc
    19441 caggctcggg cgcgtgccga tcttctcgta cagcgcgacc agcgccatca gccggtcgat
    19501 gtccgtctcg aagccgcgtt ccagcccgag ccgctccagg atctcgcagg ccggctgaag
    19561 ctggcccgtc agctcatgga tccacgcctc ggcgagcacc gcgagcttgt agaccgaggg
    19621 atcggtttcc ttgtggcgcg ccgcgacccg gcggcaggtg gcttccgcct cggtgggacg
    19681 ccggttcacg aactggcgga aggcctcctc gagggccagg taggcggtgg gcttcttcac
    19741 cagctggaac tgacccaggg cgaggaactc ccccgcgccg tgcacggggt cctgggccat
    19801 gtggagcatc gattcgaggt gcatccgccc ctgctcgccc gaaatggatc ttcgggactg
    19861 gagctgggcg gcgcaggcga cggccagcga gagctggccg cgcatgtgct tgatcttggt
    19921 gaagaggctc agcgcgtcga ggtgctcggg cgtctcctcg aggatctccg cgacctcgag
    19981 ctcggcgcgc tgcagctcgc gcatctcgac caggaccttc gcgtagcgaa ggcggtcctg
    20041 catgacgatc gtggccagtt ccacggcctg attctactcc aagcggcgcc gcgcgccagc
    20101 gaccgcttct ctagctgccg gcgccctcgg gcccgggtga cgcgaccggc attcccttgt
    20161 gccgcgccag gaaatcctcg atgaagagaa gcccctgctc tcccgcgcgc cgcaccatgc
    20221 tgaggagcat gtccatgctg gagacctcct ccagctgctc ggcgacgaac cactggagga
    20281 agttccggga cgtgtggtcg ttctccgcga tcgcgagatc cacgaggccg ttgatctgct
    20341 tggtcacggt gacctcccag tccagggaga gctgcaccgc ctcctgggcg ttcgcgaagg
    20401 cggggcgtgg cgccgcgatc gccggaatgg ctacggaacc ccccgcgtcg actacgtagt
    20461 tcacgatctt cagagcgtga accttctcct cttcggcctg gcgatagaag tgtttcgaaa
    20521 gttcgggaag gttctcccgt ccgaaatggg acgcgatctg gacgtactgg aggaaggcgc
    20581 cgaattcgtt cccgatctga gtattcagcg ccgcgaccat cttctggctg atgagcatcg
    20641 tctctcccgg gagccgccag cggcgctccg cttctacgag gtccgtccgt accggcacga
    20701 tggaaagcca agggaccgga tacttgtgcc ggactggcgc gatgttgtca agcggacgga
    20761 agggagcgcg tctcgccgat gacgctcccc aacgtacacg tttcacctct cgttgtgcaa
    20821 aagcgcgcgc gtcacggcgc gcgacctgac acgcgccggg ttcgcttcgg acgtcgcgcc
    20881 ggcgcgacgc gcgtgctcgt tccttcgcga tcgcatgaca attcctttct catgcttcgt
    20941 tttcgcatgg aaaccggatt cgttccccgg tgtttctcgg tgggtcggag cggacatcat
    21001 ctcgggggac gatgccgcac acgccgtcgc gtgttgaggg catgcgactt gcaggttttc
    21061 tcagtcgcag ttccgaatga caaacgacgt tgtgggacgc tttacggaga ggaaggtatt
    21121 cctgtcacct gtccgtgaag ccgacgcagg cgcacacacc gtgcgccgga caccccgggc
    21181 atcgagcccg ggaggaagtg agcgcatcat gtcgccgacg cgaatcccga atcccgatct
    21241 cgacgacctc cctgccgatc tccccacgga cgactccgcg cggagcgttc ccgaggcgga
    21301 gcggcatgtc cgtctggcgg accggctgaa cgaggccgag ctggaggtga tcatccaggc
    21361 cagcgcgctg cgcctgcaga acctggtcct ggtacaggct tccttcgagg acgattccga
    21421 gatcgcgtat gccgaggagg aagcatgaac gagaccgaga gcatcgacac cgccatcgaa
    21481 caggtcgaac ggctctaccg gtcggtgacc gggcagcagc cgccggtcgg agagcagccg
    21541 tacgccacga ttccccccga gaaggtcccc gaagagcacg tccaggagca ggtggaccgc
    21601 ctggtcgaga cgctcgccgc gttttccacg ggcagccgcg tcgaaaccga gtggaagcca
    21661 ttgatcacgt tgtgggaggc caaagaggag ctggtgatcg ccgtcgatct gccgggcacc
    21721 acgcgcgagc gcgtgcacgt cacggtggac cgcggcctgc tccaggtcca tggagagcgc
    21781 ccgctccgcg aaaccaagga cggcgacgga cggaccctca agtacgtgga acacccctac
    21841 ggccgtttcc gcagggtcat cccgctgccg catgccgccc gcgtggatca gctccaggcg
    21901 aacctgcatg acggcgttct cgagatccgc attcccaagc gggagtcatc cgaatcgcga
    21961 accataccca tcgcctagcg gaggcctcat gccgccgcga cgttccaaga gctcttccga
    22021 gtcgtcacgg aagacgaaat cgatcagcga gcccgaggat cggcccgctg tccacgagga
    22081 gagacccatg gaagcgaaag tcgacgttcg caagctgcag attctgaacg accggatcaa
    22141 ccagaccatc gacgccctga accaggtgcg tctctcggtg catgga
//
LOCUS       DASSLZ010000347        14743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_126311, whole genome shotgun sequence.
ACCESSION   DASSLZ010000347 DASSLZ010000000
VERSION     DASSLZ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14743
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126311"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFM00_12965"
     CDS             complement(<1..254)
                     /locus_tag="VFM00_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499203.1"
                     /translation="MTFAAPVLDQEQLVVKRGAAIAPYTTFGIGGPADVLAEVYTEKA
                     LQEVVKSAVDSGMRWLLIGGGSNLLVGDTGFRGLVIVNRI"
     gene            492..1172
                     /locus_tag="VFM00_12970"
     CDS             492..1172
                     /locus_tag="VFM00_12970"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET7499204.1"
                     /translation="MIPLTRSRQDYLKALYALSPGGEPVATSRLARRLNVSAPSVTNM
                     LARLASERLVDYAPRAGASLTSRGRREALDIVRRHRILETFLVRVLGFDWSEVHEDAE
                     VLEHHVSDRVLLAIDRLIGHPEEDPHGHPIPDRRGRISRRTLVPLSAVARGKEVRVRE
                     IRDSDAGRLARWKELGLVPGARVRVRDVREADGVTELEVAGRQVVVGQAVLEGVMVQS
                     VRRTPRGA"
     gene            1162..3171
                     /locus_tag="VFM00_12975"
     CDS             1162..3171
                     /locus_tag="VFM00_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET7499205.1"
                     /translation="MELEPDHRSLHELHRTVAVPARSSWPRRMLAFSGPAYLVSVGYM
                     DPGNWATDLAGGARFGYQLIWVLLASNLIAVLLQALSARLGVVTGKDLAQECRTAYPR
                     LLTIPLWILAEIAIVACDLAEVLGAAVALKLLFHFPLLWGVLITALDVLLLLTLQRMG
                     MRRLEALILTLIGTMGFCFAVEIALSRPDLAEVFRHLLPRGPDGQPSLFQHDPAQGWS
                     VLGLRGDSLYIAIGILGATVMPHNLYLHSALVQSRVVAQTTEGKREAVRMNAIDSAVA
                     LNLAFFVNAAILVVSAAVFHFSGHQDVARIEDAHRLLAPLLGTTLASVLFAVALLSSG
                     QSSTITGTLAGQVVMEGFLRLRIQPWLRRLISRSLAIIPAALVIGLHGENAVDSLLVL
                     SQVVLSLQLSFAVIPLVAFTSDRRRMGELANPMWMVVLGWLAAFFIAGLNAKLVGDIV
                     TSWYASTGGAWWLSALVVPAIVVIGGLLLLITALPLLQRLAPQWAGRLAPSGGVVTAI
                     PITATHVPPGPRTIVETGPRRVALALELGPADAATLDHFRGMALPADATVALMHVAES
                     AASRYLGSESLDKESREDLAVLEQLAAELRRTGLQVQVMLGNGDVKSELARMIKEWKA
                     DLVVTGSHGHRWLGDLFLGATTSGLRHRVQCPVLTIRASRSQPKS"
     gene            complement(3296..4909)
                     /gene="groL"
                     /locus_tag="VFM00_12980"
     CDS             complement(3296..4909)
                     /gene="groL"
                     /locus_tag="VFM00_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET7499206.1"
                     /translation="MAKQLLFDAAARDALQRGVDKLANTVKVTLGPKGRNVVLDKKFG
                     SPTVTNDGVTIAKEIELENPYENMGAQMVKEVATKTQDVSGDGTTTATVLTQAMVQYG
                     IRHVAAGANPMFLKRGIDRAVQAIIEDLKRQSKAIKTQEEIAQVATISANNDAEIGSL
                     IADAMDKVGKDGVITVEEAKSLDTSRDIVEGLQFDRGYLSPYFVTDPERMEVTLEDAQ
                     VLIHDKKIASIKDILPILEKVAQTGRPLLIIAEDLEGEALATLVVNKLRGILNVAAVK
                     APGFGDRRRAMLEDIAVLTGGRLITEEAGYKLENATLADLGKAKRIVIDKDDTTIIEG
                     AGKKADIKGRVDQIRKEIEDSSSDYDKEKLQERLAKLAGGVAVLHIGAATEFELKEKK
                     ARVEDALAATKSAVEEGIVPGGGVALLRAQAAVRKLEGKGDEKHGIDIVAQALEVPAK
                     QIAFNAGAEGSVIVEKIKADKGARGYNAATGEFVDMVAAGIVDPTKVVRSALQHAASI
                     ASLLLTTEAIVTDIPEEEKAPPMGGGGME"
     gene            5075..5299
                     /locus_tag="VFM00_12985"
     CDS             5075..5299
                     /locus_tag="VFM00_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002757519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET7499207.1"
                     /translation="MIKVKVRKVGNSLGLTLPSEAVQALRVREGDQLYLTEAPDGYRL
                     TPYDPDFEETMEAAEEFMAQYRNALRELAK"
     gene            5283..5702
                     /locus_tag="VFM00_12990"
     CDS             5283..5702
                     /locus_tag="VFM00_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET7499208.1"
                     /translation="MSSRNKEPRWISKKAALAICERLLAEHGGAAGIRDEGSLEAALA
                     APRNHFYYSGPDLFTLAAVHAHAITRNHPFLDGNKRVALTLAGVFLRINGFRLFASER
                     DAIVATFALSNREIGFEEFAAWLREHSKKATRRKTGR"
     gene            5699..6406
                     /locus_tag="VFM00_12995"
     CDS             5699..6406
                     /locus_tag="VFM00_12995"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7499209.1"
                     /translation="MSPSRLVDLSHVVEHGMITYKGLPAPIVCDFLSREASRAIYAAG
                     TEFHIGKIEMVANTGTYLDSPFHRFADGKDLSELPLESLADLDAVVVRCLRGGGDGAG
                     SDSELVRRDRRRIVADSFRGLDVRGKAVLVHTDWCSRWRTDAYFEGHPFLTAEAAALL
                     VEGGAALVGIDSYNIDDTEDGSRPVHTTLLGAEIPIVEHMRGLERVPDSGFRFSAVPV
                     KVKRFGTFPVRAFAAVP"
     gene            complement(6549..7379)
                     /locus_tag="VFM00_13000"
     CDS             complement(6549..7379)
                     /locus_tag="VFM00_13000"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET7499210.1"
                     /translation="MLLAIDIGNTEVTLGLFEGRKLGRSYRVSSETRRTADEAELLLR
                     QIAPEVAGSNGGAEGTRKPRGTRRAVASGHACVLASVVPPQTAIFAEAARHLMGKAPL
                     EITAASAPWLPIDYRDPGAVGADRIANAVAAIERHGKPCIIVDFGTATTFDVISREGR
                     YLGGVIAPGVLTGAENLVRRAARLGAFEVKAPPRVVGKSTEESLQSGLLYGAVGQVDT
                     IVRKIAAEARMRPIVIATGGLAKAIAPHSKTIQKVDAHLTLHGLRMIHERSGTKAGRS
                     "
     gene            complement(7390..8268)
                     /gene="tatC"
                     /locus_tag="VFM00_13005"
     CDS             complement(7390..8268)
                     /gene="tatC"
                     /locus_tag="VFM00_13005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET7499211.1"
                     /translation="MPGSISDAAATHGAGPVDAPAKAPPPPPEPAPAPEPATVAEAAG
                     AATGERVMPLLEHLDELRKALFRIAVAAIILIGGAWAFSDRLLDALIIRLLGPGQKAL
                     ALTPGEAFSARMTVALWTGGLVAVPYLLLEVWRFVAPGLKRDERKFAIPWMFASGILL
                     YAGIAFALELLLPAMVGMLQSFATPNIEARVSVGSLLGFAVKLAAGCGLLFQMPLVLC
                     LLAWFGVVSPRMLWRAWRHAVFFIALGAAVITPGDGPSMLILTAPLVVLYFLSVLVAW
                     LIWRARGRPERQGLFS"
     gene            complement(8280..8729)
                     /gene="nrdR"
                     /locus_tag="VFM00_13010"
     CDS             complement(8280..8729)
                     /gene="nrdR"
                     /locus_tag="VFM00_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET7499212.1"
                     /translation="MKCPSCGHQEDKVIDSRAAKEGQAIRRRRECLKCHRRFTTYETI
                     ETTPLFVIKKDGRREPFSREKILNGLLRACEKRPIAAERLNELVDSLEADLLRQGVDE
                     VPSHEIGARVMEALRSLDEVAYVRFASVYRHFKDLNQFLEELRSFLK"
     gene            complement(8726..9499)
                     /locus_tag="VFM00_13015"
     CDS             complement(8726..9499)
                     /locus_tag="VFM00_13015"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET7499213.1"
                     /translation="MTGPAAYLGIPIEFRRSLASTNDEVLRRAADGAPEGLVIATDEQ
                     TAGRGRQGRTWWDAPGRSLLFSVLLRPGIPLARFPLLGMAMACAMSEAGSAITGEPLA
                     VKWPNDVLHAGRKLCGILAESRPGAAGHVLVVGAGINVNQEAGDFPDELRSRATSLRV
                     AWGGRELDREDVLAAVLERFERYRGVAALDGPEALREAILPCLPQPGTRIAVQTPEGR
                     VEGTVEAILATGALLIQDATGARFPVVAGEIPFGIGESA"
     gene            complement(9546..10457)
                     /gene="nadC"
                     /locus_tag="VFM00_13020"
     CDS             complement(9546..10457)
                     /gene="nadC"
                     /locus_tag="VFM00_13020"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET7499214.1"
                     /translation="MRLEAIALPMVRLALTEDVGGGDATTDAVVDASARARAHIEARH
                     EGILAGSGVAALAFRELDPEMQIAWSCPDGGTIPKGTPLATLEGSARAILTAERVALN
                     FLQHLSGIATMAGAFVKAVEGTGVHILDTRKTTPGLRILEKYAVLAGGAMNHRFGLFD
                     GVLVKENHIKAAGSVAAAVTRAKESAQPKGLPVVVEVRSVEDAIDVAGMGVDRLLLDN
                     FTPPKIADVVKRLGLGKKTVRTKGKSGAPEAGGAAGGPPEIEVSGGVRLANVREYAIP
                     GVSYISIGALTHSAPALDLSLLLDEKL"
     gene            complement(10366..12471)
                     /gene="uvrB"
                     /locus_tag="VFM00_13025"
     CDS             complement(10366..12471)
                     /gene="uvrB"
                     /locus_tag="VFM00_13025"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET7499215.1"
                     /translation="MLDRDAPASSFKLHAPYDPRGDQPRAIRELTEGVRRGDRFQTLL
                     GVTGSGKTFTMANVIAQVGRPALVMSHNKTLAAQLYGEFKSFFPENAVGYFVSYYDYY
                     QPEAYVPQTNTYIEKDASINDDIDRLRLHATSALLERRDVVIVASVSCIYGLGSPEDF
                     QRERLFIQLGETITRERILEHLVHSQYTRGDLELARGTFRARGDVIEIHPAYEEIGLR
                     IELFGDQVDRITEIDPLTGKTRRLLTSTAIYPAKHFVTQPEKLERGLTAIREELAERL
                     AVFQSEGKLLEAQRLKMRTEYDMEMLREIGTCAGVENYSRPLSGRRPGERPQCLIDYF
                     PKDFLLIIDESHVTVPQIGGMYEGDRSRKQTLVDYGFRLPSALDNRPLRYDEFETLID
                     QAIFVSATPSAYELQKSGGVIVEQIIRPTGLVDPRVAVRPTRGQIDDLLEEIKKRVEV
                     GERVLVTTLTKRMAEDLTEYLFQAGVKVRYIHSDIDAIGRMEVLRALRLQKVDVLVGI
                     NLLREGLDLPEVSLVAILDADKEGFLRSETSLIQTAGRAARHIRGQVLLYADHVTGAM
                     RRALEEMNRRREAQVEYNAEHGITPTTIVKSVEEVMRTTSVADAAGAPRVEELLPARA
                     LDLDREGLMEVLEREMLEAASREEFELAAVFRDRLDELRLGGGKEGTRAARSDRTADG
                     APGSDGGRRGRGRDHRRRR"
     gene            complement(12542..13999)
                     /locus_tag="VFM00_13030"
     CDS             complement(12542..13999)
                     /locus_tag="VFM00_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HET7499216.1"
                     /translation="MRGWGVGAIALAAGLGLCAVAGTSDSQDLQRKRDTGTAAAFDGA
                     GDTGPGGRDLSVTVYNDNLGLVKDRRTVSVDRGRSEFSFTGVAAQLDPTSVHIRAMGN
                     GSIDVASQEYRNDFASTDRLLQRYLNRAIEVRLKGNDVRRGTLVSFDPTSVVLQTSGG
                     LSVLTRTEAQEIALASSGGDVAAGPTLVWRVNSSNGRSVPVEVSYLTGGLTWHAEYVA
                     VQDEGGGTMDLQGWATVENSSGESYENARVKLIAGAVHRASGPRPPMPYETMMRADAG
                     LAAQKLSERGFDEYHLYELPDRATIANNEVKQLGLMRARGVTTTRKYVYDAEKNTDQV
                     MTTLEFQNEASAGIGIPLPAGLVRVYQRDADGALEFTGEDRIQHTAKNETARVAVGGV
                     FDVAVERKQTEFKQISRAESEYTVEITLRNHRTKPIDVTVVEHAYGDWEIVQSSLPAT
                     KKDATKFEFSARCAPERPTTVSYTLRTRVPGVEPR"
     gene            complement(14014..>14743)
                     /locus_tag="VFM00_13035"
     CDS             complement(14014..>14743)
                     /locus_tag="VFM00_13035"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7499217.1"
                     /translation="IYDFTWREFCDWYLEMAKPRLYAGPHTPDALTVRYVIDSVFSTV
                     IRLLHPFMPFLTEELWHSLPGTEGDVTVAPWPVPARGASHPEAEARIALLKEVVGAVR
                     NLRSEMNIAPARKAAIRIRASGPDAAVLEKQRDLILLLGRGETLEVGPDTAKPKVAAS
                     AVVRNHEIFLPLEGLIDVEVERARLRKEYERVLREFDVSRRKLQNEDFLGKAKKDVVD
                     REREKFETLGTTKEKLERNLEVLQ"
BASE COUNT         2112 a         5212 c         4943 g         2476 t
ORIGIN      
        1 tcgatccggt tcacgatgac gagcccgcgg aagccggtgt cccccacgag gaggttcgag
       61 ccgccgccga tcaggagcca gcgcatcccc gagtccaccg cgctcttcac gacctcttgg
      121 agcgcctttt cggtgtagac ctcggcgagc acgtcggcgg ggccgccgat cccgaaggtg
      181 gtgtaggggg cgatcgcggc accccgcttg acgacgagct gttcctggtc gaggaccggt
      241 gcggcgaagg tcacgaaatc gctcccatgg gtgcggtgcg cgccgcgctc gtctcggagc
      301 gaagcgggcg caccgtctgc attatcggct cccggcgcca aattctgaag cgagttcatc
      361 gatacgactc ctggcgcggc ccctccattc cggatccccc cgattcgggt gacgatttgc
      421 gttttcgccg ggcggaatcc agcccgcatt tgggttgcac gaacactgct attagcctag
      481 gctaacgcgc catgatcccg ttgacgcgct cgcggcagga ctacctcaag gcgctctacg
      541 ccctctcccc cggcggcgag ccggtggcca cctcgcgtct cgcccgccgc ctgaacgtgt
      601 cggcgccctc ggtcacgaac atgctcgccc ggctcgcgtc ggaacggctg gtggactacg
      661 cgccccgcgc cggcgccagc ctcacgtcgc gcggcaggcg cgaggcgctg gacatcgtcc
      721 ggcgccaccg gattctcgag accttcctgg tccgcgtcct gggcttcgac tggtcggagg
      781 ttcatgagga cgccgaggtg ctcgagcacc acgtgagcga tcgcgtgctg ctggcgatcg
      841 accgcctcat cggccacccg gaggaagatc cccatggtca tcccattccc gatcgccggg
      901 gaaggatcag ccggcggacg ctggttcccc tatccgcggt cgctcgaggc aaggaggtcc
      961 gggtgcggga gatccgcgat tcggacgccg gccgcctcgc ccgctggaag gagctggggc
     1021 tggtgccggg ggcgcgggtc agggtgcgtg acgtgcgcga agcggacggc gtgacggagc
     1081 tcgaggtggc cgggcggcag gtcgtggtcg gccaggcggt gctcgaaggc gtcatggtcc
     1141 agagcgtgcg gaggaccccg cgtggagctt gagccggacc atcgctccct gcacgagctg
     1201 caccggaccg tcgccgtgcc ggcgcgctcg agctggccgc gccggatgct ggcgttctcg
     1261 ggacccgctt acctcgtcag cgtcggctac atggatccgg gcaactgggc caccgacctg
     1321 gccggcggcg cgcgattcgg ctatcagctc atctgggtcc tgctcgccag caacctgatc
     1381 gcggtgctgc tccaggcgct ctccgcccgt ctcggcgtcg tgacgggaaa ggatctcgcg
     1441 caggaatgcc ggacggccta tccccgcctc ctgacgatcc cgctctggat cctggccgag
     1501 atcgcgatcg tggcgtgcga tctcgccgag gtgctgggcg ccgcggtcgc gctcaagctg
     1561 ctcttccatt tcccgctcct ctggggcgtg ctcatcaccg cgctcgacgt gctcctgctg
     1621 cttaccctgc agcggatggg gatgcggcgc ctcgaggcct tgatcctgac cctgatcggg
     1681 acgatgggct tctgcttcgc ggtcgagatc gccctgtccc ggccggacct ggccgaggtg
     1741 ttccgccacc tcctgccgcg cgggccggat ggccaaccgt cgctgttcca gcacgacccc
     1801 gcgcagggct ggtcggtgct cgggctgcgc ggcgattcgc tctacatcgc catcgggatc
     1861 ctgggcgcga ccgtcatgcc ccataacctc tacctgcaca gcgcgctggt gcagagccgc
     1921 gtggtcgccc agacgacgga gggaaagcgt gaggccgtcc ggatgaacgc catcgactcg
     1981 gccgtggcgc tgaacctggc gttcttcgtg aacgccgcga tcctggtggt ctcggccgcc
     2041 gtgttccact tctcggggca ccaggacgtc gcccggatcg aggatgccca ccggctgctc
     2101 gcgcccctgc tggggacgac cctcgcctcg gtgctgttcg ccgtggcgct gctctcgtcg
     2161 ggccagtcca gcacgatcac ggggacgctg gccgggcaag tcgtcatgga gggattcctg
     2221 cgcctgcgca tccagccgtg gctgcgccgc ctgatcagcc ggagcctcgc gatcatcccg
     2281 gccgccctcg tcatcgggct gcacggcgag aacgccgtgg actcgctgct cgtgctgtcg
     2341 caggtggtgc tgagcctcca gctgtcgttc gcggtgatcc cgctggtcgc gttcaccagc
     2401 gaccgccggc gcatgggcga gctcgcgaat ccgatgtgga tggtcgtgct gggctggctg
     2461 gccgcgttct tcatcgcggg gctcaacgcc aagctggtcg gcgacatcgt gaccagctgg
     2521 tacgcgtcca cgggcggggc ctggtggctc tccgcgctgg tggtgcccgc gatcgtcgtg
     2581 atcgggggcc tgctcctgct gatcacggcg ctcccgctcc tgcagcgcct ggctccgcag
     2641 tgggccggca ggctggcgcc ctccggcggc gtcgtgacgg cgatcccgat caccgccacg
     2701 cacgtccccc ccggcccgcg caccatcgtg gagaccggtc cgcgccgcgt ggcgctcgcg
     2761 ctcgagctgg gtccggcgga tgccgcgacg ctcgaccact tccgggggat ggcgcttccg
     2821 gccgacgcca cggtcgcgct catgcacgtg gccgagagcg cggccagccg ctacctcggc
     2881 tccgagtcgc tcgacaagga gagccgtgag gatctggcgg tactcgagca gctggcggcg
     2941 gagctgcggc gaaccggact ccaagtgcag gtgatgctgg ggaacggcga cgtgaagtcc
     3001 gagctcgcgc gcatgatcaa ggagtggaaa gcggacctgg tcgtcaccgg ctcgcacggg
     3061 catcgctggc tcggcgacct gttcctggga gcgaccacct ccggcctccg ccatcgcgtg
     3121 cagtgccccg tgctcacgat ccgggcgagt cggtcccagc cgaagagctg aggggggcgc
     3181 cccgggcagt tcggtcagcg cgcggctcac cgggggccca cccggacggc gccccaacag
     3241 aacggccccg cggggaggat cccacggggc cgtcgtacgc agctgtcgaa gagggctatt
     3301 ccatcccgcc gccgcccatg ggcggcgcct tctcctcttc cgggatgtcg gtgacgatcg
     3361 cttcggtggt caggagcagc gaggcgatgc tcgccgcgtg ctgcagcgcc gagcggacca
     3421 ccttggtggg gtccacgatg ccggccgcga ccatgtccac gaactcgccg gtggccgcgt
     3481 tgtagccgcg cgcgcccttg tccgccttga tcttctccac gatcaccgag ccctcggcgc
     3541 ccgcgttgaa ggcgatctgc ttcgccggga cctcgagcgc ctgggcgacg atgtcgatgc
     3601 cgtgcttctc gtctcccttc ccctcgagct tccgcaccgc ggcctgcgcg cgcagcagcg
     3661 cgacgccgcc gccgggcacg atcccctcct cgacggccga cttcgtggcc gcgagggcgt
     3721 cctcgacgcg cgccttcttc tccttcagct cgaactccgt cgcggcgccg atgtgcagca
     3781 cggccacgcc gcccgcgagc ttcgccaagc gctcctggag cttctccttg tcgtagtccg
     3841 aggaggaatc ctcgatctcc ttccggatct ggtccacgcg ccccttgatg tcggccttct
     3901 tccccgcgcc ttcgatgatc gtcgtgtcgt ccttgtcgat cacgatgcgc ttggccttgc
     3961 cgagatcggc gagggtggcg ttctccagct tgtagcccgc ctcttcggtg atgaggcggc
     4021 cgccggtcag cacggcgatg tcctcgagca tggcgcgccg gcggtcgccg aagcccggcg
     4081 ccttcaccgc ggccacgttc aggatgccgc gcagcttgtt cacgacgagg gtggccagcg
     4141 cctccccttc caggtcctcg gcgatgatca ggagcgggcg gcccgtctgc gccaccttct
     4201 cgaggatcgg gaggatgtcc ttgatcgagg cgatcttctt gtcgtggatc aggacctgcg
     4261 cgtcctccag cgtcacctcc atccgctcgg gatcggtgac gaagtagggc gagaggtacc
     4321 cgcggtcgaa ctgcagccct tccacgatgt cgcgggaggt gtcgaggctc ttcgcctcct
     4381 cgaccgtgat gacgccgtcc ttccccacct tgtccatcgc gtcggcgatg agcgagccga
     4441 tctcggcgtc gttgttcgcg gagatggtcg cgacctgcgc gatctcttcc tgcgtcttga
     4501 tcgccttgga ctggcgcttc aggtcctcga tgatcgcctg cacggcgcgg tcgatgccgc
     4561 gcttcaggaa catgggattc gcgcccgccg ccacgtggcg gatcccgtac tggaccatcg
     4621 cctgcgtgag cacggtcgcg gtggtggtgc cgtcgcccga gacgtcctgc gtcttggtcg
     4681 cgacctcctt caccatctgc gcgcccatgt tctcgtaggg gttctccaac tcgatctctt
     4741 tggcgatcgt cacgccgtcg ttcgtcaccg tcggcgagcc gaatttcttg tcgaggacga
     4801 cgttccgccc cttgggcccc agcgtgacct tgaccgtgtt cgcgagcttg tccacgccgc
     4861 gctggagcgc gtcgcgggcc gccgcgtcga agagaagctg cttcgccatg tggttccgtt
     4921 cctccttgcc gcacgccccc caagggctgc gagatttggc actaaggtac cgagagtgct
     4981 aacggggaga gggtagcgaa gtctggaatt ctcctcaata gggttgacgc agcaaccgat
     5041 acgttataac gtaggtgata acaacggagg agccatgatc aaggtcaaag tacgtaaagt
     5101 aggtaattcc cttggactta ccctgccatc ggaagccgtt caggccctgc gcgtgcgcga
     5161 gggagatcag ctgtacctga ccgaggcacc agacggttat cgcctgactc cctatgatcc
     5221 cgacttcgag gagacgatgg aggcggccga ggagttcatg gcgcagtacc gaaacgccct
     5281 ccgtgagctc gcgaaataag gagccccgct ggatctcgaa gaaggcggcc ttggcgatct
     5341 gcgagcgcct tctcgccgaa catggtgggg ccgcgggcat tcgcgacgag ggctctctcg
     5401 aagcggcgct cgccgcgcct cggaaccact tctactactc ggggcctgat ctcttcacgt
     5461 tggcagccgt tcacgcccac gcgatcacaa gaaatcatcc cttcctggat ggaaacaagc
     5521 gagtcgcgct gacgcttgcg ggtgtatttc tcaggatcaa cggattccgt ttgttcgcga
     5581 gcgagcgcga cgcgatcgtg gccacgttcg ccctgtcgaa tcgagagatc ggtttcgagg
     5641 agtttgctgc gtggctacgg gaacattcca agaaggcaac ccgccgcaag accggccgat
     5701 gagcccgtcc cgtcttgtcg acctcagtca cgtcgtcgag cacgggatga tcacctacaa
     5761 gggcctcccc gcgccgatcg tctgcgattt cctgagccgc gaagcgtcgc gcgccatcta
     5821 cgcggccggg accgagttcc acatcgggaa gatcgagatg gtcgcgaaca cggggaccta
     5881 tctggacagc cccttccatc ggttcgctga tgggaaggat ctgagcgaac tgccgctgga
     5941 gtcgttggcc gatctggatg cggtggtggt gcggtgcttg cgcgggggtg gcgacggtgc
     6001 tgggtcggat tctgagctcg ttcgccgcga tcgccgtcgt atcgttgccg attccttccg
     6061 cggcctcgac gtgcgcggga aggccgttct cgtccatacc gactggtgct cgcggtggcg
     6121 tacggacgcc tatttcgaag gacatccctt cttgacggcg gaggccgccg ctctgctcgt
     6181 cgaagggggc gcggcgctgg tcgggatcga ttcctacaac atcgacgaca ccgaagatgg
     6241 gagccgtccc gttcacacga cgctgctggg cgcggagatt ccgatcgtgg agcacatgcg
     6301 gggactggag cgggtaccgg actcgggctt ccgattctcg gcggtacccg tcaaggtgaa
     6361 gaggttcggg acgtttccgg tccgggcttt cgcggcggtt ccgtaacccc agttaccctg
     6421 accaacttgc gccggcgcaa gttaactttt atattaactg ctcgccgaga gtcgaaccgc
     6481 tccacgctgt cgaacatgtt ctagaacatg ttcgtgtgtc agcttggcgc gattcgccac
     6541 aaaagccgct agcttcggcc ggccttcgtc ccgctgcgct cgtggatcat gcgcaaaccg
     6601 tgcagcgtca ggtgggcgtc caccttctgg atcgtcttcg agtgcggggc gatggctttg
     6661 gccaaccccc cggtcgcgat cacgatgggg cgcatccgcg cctcggcggc gatcttccgg
     6721 acgatggtgt ccacctgtcc gaccgcgccg tacagaagcc ccgactggag gctttcctcg
     6781 gtgctcttcc ccaccacgcg cggcggcgcc ttgacctcga acgcgccgag acgcgccgcg
     6841 cgccgcacca ggttctccgc tccggtcagg acgccgggcg caatcacgcc gccgaggtag
     6901 cgtccctcgc gcgagatcac gtcgaaggtc gtcgcggtgc cgaagtccac gatgatgcag
     6961 ggcttcccgt ggcgctcgat ggcggccacc gcgttcgcga tccggtcggc gcccaccgca
     7021 cccgggtcgc gatagtcgat cggcagccac ggcgcgctgg cggcggtgat ttcgagcggg
     7081 gccttcccca tcagatgccg cgccgcctcg gcgaagatcg cggtctgcgg cggcacgacc
     7141 gaggcgagca cgcaggcgtg tcccgaggcg accgctcgcc gggtgccgcg cggcttccgc
     7201 gttccttccg ccccgccgtt cgagcccgcc acctccggcg cgatctggcg caggagcagc
     7261 tcggcctcgt cggccgtgcg gcgcgtctcg ctgctcacgc gataggaacg cccgagcttc
     7321 cgcccttcga acaggcccag cgtcacctcg gtgttgccga tgtcgatggc gaggagcatc
     7381 gattcctccc tacgagaaca gcccctggcg ctcgggccgc ccgcgggcgc gccagatgag
     7441 ccaggccacg agcacgctca ggaaatagag caccacgagc ggggcggtca ggatcagcat
     7501 cgacgggccg tcgcccggcg tgatgacggc ggcgcccagc gcgatgaaga agacggcgtg
     7561 gcgccacgca cgccagagca tgcgcggtga gacgacgccg aaccaggcga gcaggcagag
     7621 cacgagcggc atctggaaca ggagcccgca ccccgcggcg agcttcacgg cgaagcccag
     7681 gagcgacccc accgagacgc gcgcttcgat gttcggcgtg gcgaaggact ggagcatccc
     7741 gaccatcgcg ggcaggagca gctcgagcgc gaaggcgatc cccgcataga gcagaatgcc
     7801 cgaggcgaac atccagggga tcgcgaactt ccgctcgtcg cgcttcaatc ccggagcgac
     7861 gaagcgccag acctcgagaa gcagataggg caccgcgacc agcccgcccg tccagagcgc
     7921 caccgtcatg cgcgccgaga acgcctcgcc cggcgtcagg gccagcgcct tctgtcccgg
     7981 gccgaggagc cggatgatca gcgcgtcgag gaggcggtcg gagaaggccc acgcgccgcc
     8041 gatcaggatg atcgcggcga ccgcgatgcg gaagagcgcc ttccgcaatt cgtccaggtg
     8101 ctcgagaagc ggcatgacgc gctcgccggt cgccgcgccc gccgcctcgg cgacggtcgc
     8161 gggctctggg gccggagcgg gctcgggtgg cgggggcggc gctttcgccg gcgcatccac
     8221 cggcccggcg ccgtgcgtcg ccgcggcgtc ggaaatcgaa ccgggcaccg gaggatcgct
     8281 catttcagga acgaccgcag ctcttcgagg aactgattca gatccttgaa gtgccggtag
     8341 acggaggcga accggacgta ggccacttca tccagcgacc gcagcgcctc catgacgcgc
     8401 gcgccgattt cgtgcgaggg aacctcgtcc acgccctggc gcagcaggtc cgcttccagc
     8461 gaatccacca gctcgttcag ccgctccgcg gcgatcgggc gcttctcgca cgcgcgcaac
     8521 agcccgttca ggatcttctc gcggctgaac ggctcccgac ggccgtcctt cttgatcacg
     8581 aagaggggcg tcgtctcgat cgtctcgtag gtcgtgaagc ggcggtggca cttgaggcat
     8641 tcgcgccggc gccggatcgc ctgcccctcc ttggcggcac gcgagtcgat gaccttgtct
     8701 tcctggtgcc cgcacgacgg acacttcacg cggactctcc gatgccgaag gggatctcgc
     8761 ccgcgaccac cgggaaccgc gcgccggtcg cgtcctggat cagcagcgcg cccgtggcga
     8821 gaatggcttc caccgtgccc tcgacgcgcc cttccggggt ctgcaccgcg atgcgcgtgc
     8881 cgggctgggg caggcagggc aggatcgcct cgcggagcgc ctccggtccg tcgagggcgg
     8941 cgacgccgcg gtagcgctcg aagcgctcga gcacggccgc gagcacgtcc tcgcggtcca
     9001 gctcccgccc gccccaggcg acgcgcagcg acgtggcgcg gctccgaagc tcgtccggga
     9061 agtcccccgc ctcctggttc acgttgatcc ccgcgccgac gaccaggacg tgccctgcgg
     9121 cgcccggccg cgactccgcg agaatgccgc agagcttccg cccggcgtgc agcacgtcgt
     9181 tgggccactt cacggcgagc ggctcccccg tgatcgcgga gccggcctcg gacatcgcgc
     9241 acgccatggc catcccgagc agcgggaacc gcgcgagggg aatccccggc cggagcagca
     9301 cggagaagag aagggaccgc ccgggcgcgt cccaccacgt ccggccctgc cggccgcgtc
     9361 ccgccgtctg ctcgtccgtg gcgatcacca gcccctcggg ggcgccgtcg gctgcgcggc
     9421 gaagcacctc gtcgttggtc gaggcaaggc tgcgccggaa ttcgatcggt attccgaggt
     9481 aggcggcggg gccggtcacg accgaacccc gcggcggctt cgcatcccct cgcttcgctc
     9541 gggggtcaca gtttctcgtc caggagcagc gagagatcga gcgccggcgc cgaatgggtc
     9601 agcgccccga tcgaaatgta ggagacgcca gggatggcgt actcgcgcac gttggcgagc
     9661 cgcacgcccc cggagacctc gatctcgggc ggcccccccg cggcgccgcc ggcctcgggc
     9721 gcgccgctct tccctttcgt gcgcacggtc ttcttgccca gacccagccg cttcaccacg
     9781 tccgcgatct tcgggggcgt gaagttgtcg agcagcagac ggtccacacc catgccggcc
     9841 acgtcgatgg catcctccac cgatcgcacc tcgaccacga ccggaagccc cttcggctgg
     9901 gccgattcct tggcgcgggt cacggcggcc gcgacactgc ccgctgcctt gatgtggttc
     9961 tccttcacga gcacgccgtc gaagagcccg aagcggtggt tcatggcgcc gccggcgagc
    10021 acggcgtact tctcgaggat gcggaggccg ggggtcgtct tccgggtgtc caggatgtgt
    10081 acgcccgtgc cttccacggc cttcacgaaa gcgcccgcca tcgtggcgat ccccgacagg
    10141 tgctgcagga agttcagcgc cacgcgctcg gcggtgagga tggcccgcgc cgatccctcg
    10201 agcgtcgcga gcggagttcc cttgggaatg gttccgccgt cggggcagga ccacgcgatc
    10261 tgcatctcgg gatccagctc gcgaaaggcg agcgccgcga cgccgctccc cgcgaggatc
    10321 ccctcgtgcc gcgcctcgat gtgggcccgc gcccgggccg atgcgtcaac gacggcgtcg
    10381 gtggtcgcgt ccccgccccc gacgtcctcc gtcagagcca ggcgcaccat cggcagtgcg
    10441 atcgcttcga gccgcacgcg ttccttcctt tccccctccg agccgtagct cgtccagccg
    10501 gtcgcggaag accgcggcca gctcgaattc ctcgcgcgac gccgcctcga gcatctcgcg
    10561 ctcgagtacc tccatgagtc cctcgcggtc caggtccagg gcgcgcgcgg ggagcagttc
    10621 ctccacgcgc ggcgcgcccg ccgcgtcggc gacggacgtg gtccgcatca cttcctccac
    10681 cgacttcacg atggtcgtcg gcgtgatgcc gtgctcggcg ttgtactcga cctgcgcctc
    10741 gcgccgccgg ttcatctcct cgagcgcgcg gcgcatggcg ccggtcacgt ggtcggcgta
    10801 gagcagcacc tggccccgga tgtgccgcgc ggcgcgcccc gcggtctgga tcagggacgt
    10861 ctccgagcgg aggaacccct ccttgtcggc gtccaggatc gcgaccagcg acacctcggg
    10921 caggtccagc ccctcgcgca ggaggttgat ccccaccagc acgtccactt tctggagacg
    10981 cagcgcccgg agcacctcca tccggccgat cgcgtcgatg tcggagtgga tgtagcgcac
    11041 cttcacgccg gcctggaaca gatactcggt caggtcctcg gccatccgct tggtgagcgt
    11101 ggtcacgagc acccgctccc cgacctccac gcgcttcttg atctcctcca gcaggtcgtc
    11161 gatctgcccg cgcgtcggcc gcaccgccac gcgcggatcc accagacccg tgggccggat
    11221 gatctgctcc acgatcacgc cgcccgactt ctggagctcg taggcggagg gggtcgcgga
    11281 gacgaagatg gcctggtcga tcagggtctc gaactcgtcg tagcggagcg ggcggttgtc
    11341 cagcgccgac gggagccgga aaccgtaatc caccagcgtc tgcttgcgcg agcggtcccc
    11401 ctcgtacatc ccgccgatct gcggcaccgt gacgtgcgac tcgtcgatga tgagcaggaa
    11461 gtccttcggg aagtagtcga tcaggcactg gggccgctcc cccggccggc gcccggacag
    11521 cgggcgcgag tagttctcca cccccgcgca ggtgccgatc tcgcgcagca tctccatgtc
    11581 gtattcggtc cgcatcttga gccgctgcgc ctcgaggagc ttcccttcgc tctggaagac
    11641 ggccagccgc tcggccagct cctcgcggat cgccgtgagc ccgcgctcca gcttctcggg
    11701 ctgggtcacg aagtgcttcg cgggatagat cgcggtgctc gtgaggagcc gccgcgtctt
    11761 gcccgtgagc gggtcgatct ccgtgatccg gtccacctgg tccccgaaca gctcgatccg
    11821 gagcccgatc tcttcgtagg cgggatggat ctcgatcacg tccccccgcg cgcggaacgt
    11881 cccgcgcgcc agctcaaggt cgccgcgcgt gtactggctg tgcaccaggt gctccaagat
    11941 ccgctcgcgc gtgatcgtct cgcccagctg gatgaagagc cgctcgcgct ggaaatcctc
    12001 gggcgagccg aggccgtaga tgcaggagac gctcgcgacg atgaccacgt cgcgccgctc
    12061 caggagcgcg ctcgtggcgt gcaggcgcag gcggtcgatg tcgtcgttga tcgaggcgtc
    12121 cttctcgatg taggtgttcg tctgcgggac gtacgcctcg ggctggtagt agtcgtagta
    12181 ggagacgaaa tagcccaccg cgttctcggg aaagaacgac ttgaactcgc cgtagagctg
    12241 cgcggcgagc gtcttgttgt gcgacatgac cagcgcgggg cggccgacct gggcgatgac
    12301 gttcgccatg gtgaacgtct tccccgatcc agtgacgccg agcagcgtct ggaagcggtc
    12361 gccgcgccgg accccctcgg tcagctcgcg gatggcgcgc ggctggtcgc cgcgggggtc
    12421 gtacggcgca tgcagcttga acgacgacgc gggcgcgtcc cggtccagca ccccggtcac
    12481 cagggctccg cgaccggccc gagcgcgacg gccggccgcg aggtcacgcc tgcggtttcc
    12541 gtcaccgcgg ctccacgccc gggacgcgcg tccggagcgt gtagctcacg gtcgtggggc
    12601 gctcgggggc gcaccgcgcg ctgaactcga acttggtcgc gtccttcttg gtcgccggca
    12661 ggctggactg gacgatctcc cagtcgccgt aggcgtgctc caccaccgtg acgtcgatcg
    12721 gcttcgtgcg gtggtttcgc agcgtgatct ccaccgtgta ttcgctctcc gcgcgggaga
    12781 tctgcttgaa ctcggtctgc ttccgttcca cggccacgtc gaagacgccg cccaccgcca
    12841 cgcgcgccgt ctcgttcttg gcggtgtgct ggatccggtc ctcgccggtg aactccagcg
    12901 cgccgtccgc gtcgcgctgg tagacgcgca cgagccccgc gggcaggggg atcccgatcc
    12961 ccgccgacgc ctcgttctgg aactccagcg tggtcatcac ctggtcggtg ttcttctcgg
    13021 cgtcgtagac gtacttgcgg gtggtggtca caccccgcgc gcgcatcagc ccgagctgct
    13081 tcacctcgtt gttcgcgatc gtcgcgcggt cgggcagctc gtacaggtga tactcgtcga
    13141 agccgcgctc ggacagcttc tgcgccgcca gcccggcgtc cgcgcgcatc atcgtctcgt
    13201 agggcatcgg cgggcgcgga ccggaggcgc ggtggaccgc cccggcgatc agcttcaccc
    13261 gggcgttctc gtaggactct cccgagctgt tctcgaccgt ggcccagccc tggaggtcca
    13321 tcgtgccgcc cccctcgtcc tgcacggcga cgtactcggc gtgccacgtc aggccgcccg
    13381 tcaggtacga gacctcgacc gggacggagc gcccgttcga ggagttcacg cgccagacca
    13441 gcgtcggtcc ggcggcgacg tcgccgcccg aggaagccag ggcgatctcc tgcgcctcgg
    13501 tccgggtcag caccgagagc ccgccggaag tctgcaggac tacggacgtg ggatcgaagg
    13561 agacgagcgt accgcggcgc acgtcgttac ccttcagacg cacctcgatc gcccggttca
    13621 gataacgctg gaggagccgg tccgtcgagg cgaaatcgtt ccggtattcc tgcgaggcga
    13681 cgtcgatcga cccgttcccc atcgcgcgga tgtgcaccga cgtgggatcc agctgggccg
    13741 ccacgcccgt gaacgaaaac tccgaacgcc cacggtcgac ggagaccgtc cgccggtcct
    13801 tcaccaggcc caggttgtcg ttatagaccg tgaccgacag atcccgtccg cccggaccgg
    13861 tgtcgccggc gccgtcgaac gcggcggcgg tgcccgtatc ccgcttccgc tgcagatcct
    13921 gcgaatcgct cgtccccgcg accgcgcaca acccaaggcc cgccgccagc gcgatggccc
    13981 ctacgcccca gcccctcact ccattccgtg gcttcactgc agcacctcca gattccgttc
    14041 cagcttttcc ttcgtcgtgc ccaaggtttc gaacttttcc cgctcgcggt cgaccacgtc
    14101 cttcttcgcc ttgcccagga agtcctcgtt ctgcagcttc cgccgggaga cgtcgaactc
    14161 gcggagcacc cgctcatatt ccttacgcag acgggccctc tcgacttcca cgtcgatgag
    14221 accttccagg ggcagaaaga tctcgtggtt ccggacgacg gcggaggccg ccaccttggg
    14281 cttggccgtg tcggggccca cctcgagcgt ttccccgcgg cccaagagca ggatcaggtc
    14341 gcgctgcttc tcgagcaccg cggcgtccgg accggaggcg cggatccgga tggccgcctt
    14401 gcgggcggga gcgatgttca tctcggagcg aaggttgcgc acggcgccca ccacctcctt
    14461 gagaagcgcg atccgcgcct ccgcttcggg atgggacgcg ccgcgcgccg gcaccggcca
    14521 gggggcgacc gtcacgtccc cctcggttcc gggcagcgag tgccacagct cctcggtcaa
    14581 aaacggcatg aacgggtgga gcagccggat cacggtgctg aacaccgagt cgatcacgta
    14641 gcgcacggtc agggcgtcgg gcgtgtgcgg cccggcgtac agccgcggct tggccatctc
    14701 gaggtaccag tcgcagaact cccgccaggt gaagtcgtag atg
//
LOCUS       DASSLZ010000348        23671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_743604, whole genome shotgun sequence.
ACCESSION   DASSLZ010000348 DASSLZ010000000
VERSION     DASSLZ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 23671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23671
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743604"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFM00_13040"
     CDS             <1..1215
                     /locus_tag="VFM00_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7499218.1"
                     /translation="DDLVAAYAAVLDANKAEQKADIWAARDVTLDDSAKLSPVVVGIW
                     DSGVDVSLYPKQLWVNAKEIPGNQKDDDKDGYVDDVNGIAWSLHSDKETALLFDIDKA
                     VGNKDVYKGDLKGFEDLQANLDTPEATALKQKLSGLTRDQVQPFLEGLNAYAQYAHGT
                     HVAGIATRGNPAARVLACRITFDYHSIPEKPTVEQAVKDGDAMAKSVAYFKANGVRVV
                     NMSWGGDLKSIETALEQNHAGGTPEQRRALARKLYDIDYKKLYDAVKGAPGTLFVIAA
                     GNSDNNVKFDEVMPSSFKLPNVLVAGAVDQAGDQTSFTSFGNVDVYSSGFEVDSYVPG
                     GDRMKLSGTSMAAPNVTNLAAKLWALNPKLTVEQVKQYIVDSAEEKKAGDKTIRLLNP
                     KASLSAASGVAP"
     gene            complement(1267..1689)
                     /locus_tag="VFM00_13045"
     CDS             complement(1267..1689)
                     /locus_tag="VFM00_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499219.1"
                     /translation="MTRKQTILGVSLLFLVCLGIGVIWTLRTQPPSANPWWAGKDAKV
                     RMEVWEPGKDMATFAMTLPKKSIDGMVALGLRTVVQVGNQDLDFRKHWREIQALPPGE
                     KYRIPGDDGETTYVWIETPDVKASPVRPAAAAPESTGT"
     gene            complement(1766..2644)
                     /locus_tag="VFM00_13050"
     CDS             complement(1766..2644)
                     /locus_tag="VFM00_13050"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET7499220.1"
                     /translation="MTFLSVMALPLLACALLALILPSLGQHVLARGVIFVDLALAQIA
                     ALGQSVAFLMGAEPHDPSTYLWSFGFTLLGAALFSFLWDREHSVLQEAFIGISFALAT
                     ASTLLLLSNAPHGAEHVSGALSGEALGWVTWKDIAIMATLFAAIGLFLFLARRKLLLC
                     SEDPKAARRMGLSVKKWDFLFYASFGLVVTTAVRVSGVLAVFSFLIVPVVCSTLLGRR
                     GASRLIWAWAIAFVASMLGAGLSYLKDWPMGATIVCLFGATVAVVSLSVRMKYVDDRA
                     TGAEPPSEGIRFTGTR"
     gene            complement(2641..3573)
                     /locus_tag="VFM00_13055"
     CDS             complement(2641..3573)
                     /locus_tag="VFM00_13055"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HET7499221.1"
                     /translation="MKSIVRLALWLSLSLGLASPAWADLKVATSLTDLASVAQFVGGK
                     HVTAQSLCRGFEDPHFVPAKPSLMKAIQHADVYVSTGLELDGGWLPLVLPGSRNPKIQ
                     QGAKGFVDASRGVSVLEKPTGTVSRAAGDIHPLGNPHYYLDPKALEVVADHLANVFSG
                     LDPANAAEYAANAKAFDERMETSLKKWETEMAPYRGATIVPYHLNFIYFANRFGLKLF
                     GTVEPKPGIPPNAHYIAQLSEDMKKSGVKVVTYQPYYNADAAKQLAKRAGGVAVEVPT
                     EAGGVPGTDDVFSKFDFIVSSLTRALSGNQGGSR"
     gene            complement(3613..4623)
                     /locus_tag="VFM00_13060"
     CDS             complement(3613..4623)
                     /locus_tag="VFM00_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499222.1"
                     /translation="MFSNLANPAIGMNALFSVQAAHNLPRAYGLDFDEAEISLLSVVD
                     PYWTFTSNIVFLPDHTIDPEEVWVRNTSLTSIQLKLGQLRGTFGKHGLLHTHAFPFIQ
                     APIMMGNTIGEEGFKDPGLEASWLTPLPWYAELTGGAYKAVGVDDDHPLDLGSDRHDN
                     IPLLGHFKSQIDLNDETTMELGQSILEGRGSDGHRHAAYGADATIRNVPARSSNRKGW
                     ILQGEYIQKASYPGGSYHREADGGYASFQYRLSQVWWGGIRGERARRSVTDFMVDGAG
                     DPIAGNVSRGSVNIAWTPSEFSFIRLEYSHAKADRGIHPTDDRIMVQMSYTIGFHPAH
                     AY"
     gene            complement(5280..5453)
                     /locus_tag="VFM00_13065"
     CDS             complement(5280..5453)
                     /locus_tag="VFM00_13065"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HET7499223.1"
                     /translation="MPGKGGGTGIAFPLTLITIGIVLLLKELGYLHGNILRYWPVLLI
                     VWGIGMIWAARKG"
     gene            complement(5490..6416)
                     /locus_tag="VFM00_13070"
     CDS             complement(5490..6416)
                     /locus_tag="VFM00_13070"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HET7499224.1"
                     /translation="MVPLPPVGFAKLVFGVLLAGLGVILLAGHLGYLPTDTGGWLLRY
                     WPALLVLIGLALLASALRNPLIGWIATILVLGALGFGAWWVHQHGTGTRAETAKTYDL
                     TRPRTETLTVRARVFGGSLSLEPNAASRAGRMDVIATGMPSDDADFTTSGGAAIFDWP
                     TEDARVYQAPLGGNVRVLVPQRFRVRLLAKSLFSQVRAALARLRPERCEVEAIASSVR
                     IDATGPARPSLVLLRGTLANVELDLPASCPARLEFDPLTFRTLPDDFVEHAVGRSKVK
                     IWTSEGSGPVVHVRVAGPLIHLKVKREPVRAL"
     gene            complement(6483..6776)
                     /locus_tag="VFM00_13075"
     CDS             complement(6483..6776)
                     /locus_tag="VFM00_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499225.1"
                     /translation="MRRWVPLVALVLAGCAHAPRTPQERVAVTEEGYAWRLLERRCQG
                     CHALPDPGKYSRERWVKGVERMRKRFAMTPSDWDTLLALVPADSASAIPEVPR"
     gene            complement(6773..7594)
                     /locus_tag="VFM00_13080"
     CDS             complement(6773..7594)
                     /locus_tag="VFM00_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499226.1"
                     /translation="MPTRNERVMRRVVAVVFALALIPAILPGAARADRKYFVETYTPY
                     LAPAGEIEMETWLTSRSGKEEDEGLTAWEWRQEFEYSVTDRLTAAAYLNFDQPGGGTL
                     HFEAPSLEFIYALADPGRIPGHPALYLEAKESGEELELEPKLLLAHRSRRFVGAMNLV
                     GEFEFRHNDEELLESGAVMRKAWAWKVVGGTAYELSPRFSAGVEARYVAEYPNFGPRS
                     GSALSLGPVFNVQAGEVQIGVGVLRQLRGSPRTSGDLNLDDFERTQVRAIIGVEL"
     gene            complement(7578..8141)
                     /locus_tag="VFM00_13085"
     CDS             complement(7578..8141)
                     /locus_tag="VFM00_13085"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET7499227.1"
                     /translation="MTSAKVRQRPGLSVSSEHYLRTILELREERGYARVVDIAARMGL
                     TKGSVSVALAHLAERGLVRFDAAHFPLLTTAGRRVALDVRGRFQIVLTFLTEVLGLPV
                     PLATAEACRWEHVVSHDVADRILDFIRFTADTHGQEQLLASFAEYRRSCASGESCSTC
                     SVHGPLRLYCAHEADDRNQGGTSADTK"
     gene            complement(8332..8691)
                     /locus_tag="VFM00_13090"
     CDS             complement(8332..8691)
                     /locus_tag="VFM00_13090"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET7499228.1"
                     /translation="MLADAAVTPAIAAPAAPAAPRWKLMTTPTPTRRGEAFRIQLAGA
                     TPETTPPDLVARIFDVQGRTVARFDRAGLEFTNGGASFRWDGTDPAGRSVGAGVYFVR
                     VEAASASYREQRKVVMR"
     gene            complement(8713..10038)
                     /locus_tag="VFM00_13095"
                     /pseudo
     CDS             complement(8713..10038)
                     /locus_tag="VFM00_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012484724.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10258..10917
                     /locus_tag="VFM00_13100"
     CDS             10258..10917
                     /locus_tag="VFM00_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7499229.1"
                     /translation="MPDGASPEGPLLAKLRGGDEAAFAALVDGLNCRLQALAATFTSS
                     PALAEDIVQETWLGVIRGLDGFEGRSSLRTWIFSILIRRARTLAAREARRADAVSTNG
                     AADHEEWDVGRGRVGLWEGAPEPWALDTPEQVLQGREALEIVRTALDGLPESQRQVVL
                     LRDVEDVSPPDICNILDLTETNMRVLLHRGRARIRRALDLYLREGTRPSTAPTPVQET
                     R"
     gene            10914..11141
                     /locus_tag="VFM00_13105"
     CDS             10914..11141
                     /locus_tag="VFM00_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7499230.1"
                     /translation="MNCKEALDVVSEALDETLEESARREFLEHMDRCSACRSYVEQLR
                     ISVQALRSLPKSGRPNLRRAEIIEAYRKKFR"
     gene            11246..11995
                     /locus_tag="VFM00_13110"
     CDS             11246..11995
                     /locus_tag="VFM00_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7499231.1"
                     /translation="MGELTGKKALVTGGTRGIGRAVALDLADMGADVAINFFRSRESA
                     KAATAEIESRGVRALALRANIGNEEQIPAMFEQLKAEFGTLDILVSNAALGHFGNVLD
                     VDDKMWDIAMNTNAKALLFSAQQAVKMMPEGGKIVALTSLGSHRYIPGYASIGVSKAA
                     IETLVRYLAYELAPKKINVNAVSGGFIDTDSLKIFPSYEEIIVESTRRTPFGRVGTPK
                     EVANMVAFLCTDKASWVTGQTIIVDGGYTLG"
     gene            complement(12008..12514)
                     /locus_tag="VFM00_13115"
     CDS             complement(12008..12514)
                     /locus_tag="VFM00_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499232.1"
                     /translation="MTTLRPLDSPEPDVPRRLRRPWQVYAMVMLWAIKGFQELLSGVI
                     GTSFFVAAQSAEGRLAGYALQTAFQSIFFSTLLAVGSFYVMAGIWLGKRAARPWGIVV
                     ALVSELTLLAYLITRPPEFGGTVPLVRTVITGSFVNLGIVGFLLFDGRLTAFLGSPPL
                     TGWWSPRR"
     gene            complement(12511..12996)
                     /locus_tag="VFM00_13120"
     CDS             complement(12511..12996)
                     /locus_tag="VFM00_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7499233.1"
                     /translation="MNPSQAEHKFECHLLWTGASKGPTVDYATYSREHRVEFDGKPPL
                     TMTSAAVFRGDPAYADPEDLLVAALASCHFLSYAALCARKGVRVTAYEDDAVGIMTRG
                     EKTFHFTDVLLRPRVTIAPDSDAALARDLHHRAHTECFIAASVNFPVRHEPTIIVAAP
                     V"
     gene            complement(12993..14390)
                     /locus_tag="VFM00_13125"
     CDS             complement(12993..14390)
                     /locus_tag="VFM00_13125"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7499234.1"
                     /translation="MPRLRNASRPMLRWIDTVLPRRALFDKDRRLLHLFLGRFLASTG
                     FSVVIPFLALYLHGTRGVSMSAVGSLFFFGALCGVAGQVVGGEWSDRSGRKRVLVTCQ
                     VIRSVTFAGLGYAVTVHAPIVMFALLTGLSAFAGRMFEPPSGAMIADITSGERRVEAY
                     GVLRIGGNLGWAVGPALGGFLAALSYASLFYVASGVLLTAGILIAVLVEETHPRRRRR
                     REDAAGPERVAIPPVGPVGAGRGLSMAGTLAVLRDTLFLRYCLVTLLLFTVMGQLMAT
                     FSVYAVDWAGRTKVELGAIYTLNGVMVVLLQFPVTRILAPLRMTSALIVGCLLYSIGY
                     GMMGWGSSFALLLAGMFVVTAGEITCSPASMNLVANFSPEDQRGRYMGVYGIFNSFGW
                     SVGPLVGGVLLDLAAGRPKLLWSAIASLTVLAAIGYADLRRRIDPALDQSPEAATARP
                     AIMSRRPQGAGKEFR"
     gene            14458..15492
                     /locus_tag="VFM00_13130"
     CDS             14458..15492
                     /locus_tag="VFM00_13130"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET7499235.1"
                     /translation="MTPRDDTGVRRTRPNPEVRMDDLGPWRPAPREGAPGHYPAQDKV
                     MPLADAVRRFVLPGATVALGTCLEQMIPFAATRELIRAGIGDLTLVGPVSDICFDQLI
                     GAGLARRVMAAWVGNVMMGSAYAFRRAAERGEPRPIEVVDFSNFTLALALHAAAIGAP
                     FIPTRSALGTDWLDRNPWLLRVEDLVNGGPVLAVRPLAPDVTIIHAQRADPHGNAVMW
                     GSLGITPDAARAARAVIVTAEEIVSPERIRSDPNRVVVPGLLVAAVCEARWGAHPSPV
                     QGYYRRDHEAYAAYHERTKTAEGFAAWRAEWVDGAPDLAAYVARVGNERIHSLIPLEH
                     RFADPVDYGY"
     gene            15482..16972
                     /gene="pabB"
                     /locus_tag="VFM00_13135"
     CDS             15482..16972
                     /gene="pabB"
                     /locus_tag="VFM00_13135"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET7499236.1"
                     /translation="MDTDRSSSALLPATPELRAKAQVRTLPEWLTPMRALAAVEDAPH
                     TILFESAGAPGASSEWTLLAFDPPWRLEVAEGALWEVRGERRARRPGAPLEALAAAWP
                     CRAEIEDAPSVPFLSGLAGAIAYDFKDWIERYPDRARRERPTPDLSLGFYDCVWAWSR
                     ATGEGWCVSTGLAENDPGRRERRAGEVLEAQWSRVTAGGVADGSGLGSVVRAGAIRSN
                     FTRDAYCRMVEVALEHIAAGDIYQVNLSQRFLADSAPRPATLYRSLREAAPAPFMAYL
                     SLEEGGVASSSPERFFRVRGRHIETWPIKGTRPRGATPEEDTALRLELIHSDKDRAEN
                     VMIVDLERNDLGRVCAIGSVAVPTLCEVASHSNVHHFESRVVGTLREDAAPVDVLRAL
                     FPGGSITGTPKIRSVQIIDRLEPVRRGIYTGALGYWDVRGDGDWNIAIRTIVAGGGTA
                     SFHVGGGIVADSTPEGEYEETLVKASGMMRALGVRAPSPSTSSPRA"
     gene            complement(16950..19664)
                     /locus_tag="VFM00_13140"
     CDS             complement(16950..19664)
                     /locus_tag="VFM00_13140"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7499237.1"
                     /translation="MKAKVFQEITGLTIAIASVAAAILLRSLLDPILDDKLALITLYG
                     AVAITVWFAGLRYAVITTLAGYVLANDLFIQPRGSFHIGDPASLVGFTLYLVSCSLII
                     FFGERANRAERQRHDTDEQLQLALGAGQIGTWDWDLRTGKIHASPEARRMLGTSRFGS
                     IEEGLASVHPEDRPAIQAALERAIVNGKPFAAEARYTRPDGVVLRLSGHGVVRVDRQG
                     RPIRIVAATIDQTETWRAAESLRHERELLRRIIDTIPVMITMFDPEATMLRLNPEFTR
                     VVGWSTEEAKGVSLMEEIYPDPAYRRQVLDFMESCREGWMDVRMRTRDGRTIETSWAN
                     IRLSDDTRVGIGIDITERKRAEVALRTRNRRLRLLFEAAAVTLTSDDPDAMLHALFAK
                     IRPELALDGYWNYMLDESGTTLLLASYEGAPPDVMREADRVPLGQGVNGKVAVTRHPI
                     VRTRVQESSDGEDGVARALGLRALICHPLAAGDRLIGTLTLASRSRDTFEPDELEFAE
                     TICQHVTLAYERIRHVRELKESGRMKDEFLATLAHELRNPLAPIRNAADILKQHGPRD
                     PELVWARQVIERQAEHLSRLVDDLLDVSRITRGKIELRRERIPVSVIVSSAVETARPL
                     IEGNQHALTVSLPGEPLYVNGDLTRIAQVVSNLLTNAAKYTPPSGRIEVGAEREGDQA
                     VLRVKDNGVGIPREMLGRIFEMFAQVDSSLERTAGGLGIGLTLARTLVELHGGSIEAR
                     SDGAGRGSEFLVRLPLATTEPPPLPPPAATEGPGARDARARILVVDDNVDAADSLGRL
                     LALDGYDVRIANGGMEALREAETFRPQVILLDIGLPMMNGYDVARRLRKEPWGAEIRL
                     VALTGWGQDEDRRRSKEAGFDEHVVKPIDPASLRVLLRRVRATRVGST"
     gene            19730..20371
                     /locus_tag="VFM00_13145"
     CDS             19730..20371
                     /locus_tag="VFM00_13145"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7499238.1"
                     /translation="MRAMILPQQAPIGSAPLVLRDVPEPEPAPGEVRVRVRACGMCRT
                     DLHVIEGDLPPRKLPLIPGHQVVGVMDRAGPGAARFREGDRIGIAWLRHTCGVCVYCR
                     SGRENLCESSRFTGYHADGGYAEVAVVPEAFAYAIPDVFGDAEATPLLCAGIIGYRAL
                     ERSQAPPGGTLGLWGFGSSAHVTIQVARARGIQVYVATRGEKHQALARAMGAV"
     gene            20405..20728
                     /locus_tag="VFM00_13150"
     CDS             20405..20728
                     /locus_tag="VFM00_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499239.1"
                     /translation="MDGGILVAPAGEIVPIALRSIQKGGTLAIAGIWLSDIPPLNYER
                     ELFYERNLRSVTANTRADGEALLREAAAIPLRPRVTTFPLEEANRGLALLKSDGLNGT
                     GVLVP"
     gene            complement(20798..21337)
                     /locus_tag="VFM00_13155"
     CDS             complement(20798..21337)
                     /locus_tag="VFM00_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499240.1"
                     /translation="MKNLRWILPCLALVLFAPGCILTSGQFTLDFDLPDITATSSTGI
                     VGEQIDLNDDSDYKDHKDNVKDISDFAVLGTFHNTGTSDVNVEVYMTRDLTTLTDALT
                     ITTTGIKLWGPFKVPANGTKTIDWDASAALFTAAGKAAVLQEAKGDGNFTLYAVGSAG
                     TYQFDVENGKLVLVIDVGI"
     gene            complement(21351..21896)
                     /locus_tag="VFM00_13160"
     CDS             complement(21351..21896)
                     /locus_tag="VFM00_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7499241.1"
                     /translation="MMRTVALAVLALALAAGVASAAPVGVGVGAFGGLSYPILQDDVG
                     NGSVYGLRVPVRLLPMITVEPYWLTGKMDDATETIGGVEYTRDGFDNKGYGANAILGH
                     VGGAGFHFYPFAGIGSHKLTRTGTPEIKETAYNFGLGFGLSPTPKVSLQIRGEVNMIK
                     TGDTSRKFGNATVGLNYDLLP"
     gene            complement(21969..22742)
                     /locus_tag="VFM00_13165"
     CDS             complement(21969..22742)
                     /locus_tag="VFM00_13165"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET7499242.1"
                     /translation="MTPPVAPRERMVLRAAREIQEGETVFVGTRLPLLAYLVAKRTHA
                     PHAVALYENGLIREEAARELLFTMGDPPNILDATMASTTIDVMGMLAAGRVDLGLLGG
                     AEVDRFGNVNSTEVPGRDGGITRLPGSGGASDIIALARRTVILMPQEKRRLVPRVTFR
                     TSPGRGDGPGWRAAQGLPPGGPSAIVTTHAVFRFDAAIEEAFLSEVYPGLSIDHVMAG
                     MSWEPRIQQPIGLTPPFTEDEIGALRAIDPGGFWTGTRT"
     gene            complement(22753..23562)
                     /locus_tag="VFM00_13170"
     CDS             complement(22753..23562)
                     /locus_tag="VFM00_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7499243.1"
                     /translation="MTPETMAAPSGLCTYRTESGIAVLELCDPPANTYTYEMMRALDE
                     AILKARFDENVHVILLRGAGDKFFCAGANIKMLQTANPVFKYYFCLHANETLNRLEQT
                     PKLVIAALNGHCVGGGLEIAMAADIRIAKKGAGKIGLPEVTLGVLPGTGGTQRLGRLV
                     GKPRAIEMMALGSTFSFEEAEQMGLVNHVWDADGFDAKIMEFARQFTPPNKASKAVGR
                     IKRSVQSGLEAAFGEGLAIERELQQQLFQSEDAKEGLTAYVEKRAAKFSGK"
BASE COUNT         3857 a         7968 c         8210 g         3636 t
ORIGIN      
        1 gacgatctgg tggcggccta cgccgccgtc ctcgacgcga acaaggcgga gcagaaagcc
       61 gacatctggg cggctcgcga cgtgacgctg gacgactccg ccaagctctc gccggtcgtg
      121 gtcgggatct gggattcggg cgtggacgtc tcgctctacc ccaagcagct gtgggtgaac
      181 gcgaaggaga tcccggggaa ccagaaggac gacgacaagg acgggtacgt cgatgacgtg
      241 aacgggatcg cgtggtcgct ccattccgac aaggagacgg cgctcctctt cgacatcgac
      301 aaggcggtcg ggaacaagga cgtctacaag ggggacctca agggcttcga ggacctccag
      361 gcgaacctgg acacgcccga ggcgacggcg ctgaagcaga agctgagcgg cctcacgcgc
      421 gaccaggtgc agcccttcct ggagggactg aacgcctacg cgcagtacgc gcacggaacg
      481 cacgtcgcgg gcatcgcgac gcggggcaac ccggcggcgc gcgtgctcgc ctgccgcatc
      541 acgttcgact atcactcgat tccggagaag ccgaccgtcg agcaggcggt gaaggacggg
      601 gacgcgatgg cgaagtcggt ggcctacttc aaggccaacg gcgttcgtgt ggtcaacatg
      661 agctggggcg gcgatctcaa gtcgatcgag acggccctgg agcagaacca cgccggcggc
      721 acgcccgagc agcggcgcgc gctggcccgc aagctgtacg acatcgacta caagaagctc
      781 tacgacgcgg tgaagggcgc gccgggcacc ctcttcgtca tcgcggcggg gaattccgac
      841 aacaacgtga agttcgacga ggtgatgccg agctcgttca agctgccgaa cgttctcgtc
      901 gccggcgccg tcgaccaggc gggggaccag acgtccttca ccagcttcgg caacgtcgac
      961 gtctactcca gcgggttcga ggtcgacagc tacgtgcccg gcggcgaccg gatgaagctg
     1021 agcgggacga gcatggcggc gccgaacgtc accaacctgg ccgcgaagct ctgggcgttg
     1081 aatccgaagc tgacggtcga gcaggtgaag cagtacatcg tggacagcgc cgaggagaag
     1141 aaagcggggg acaagacgat ccggctgctg aatccgaagg cttcactgtc ggccgcgtcg
     1201 ggcgtcgcgc cgtaatccgg ccttcgtaaa gggactccgg gggagagccc gcgtcgggaa
     1261 cgacggctag gtgccggttg actccggagc cgcagccgcg ggccggaccg ggctcgcctt
     1321 cacgtccggc gtctcgatcc agacgtaggt cgtctccccg tcgtcgcccg ggatccggta
     1381 cttctcgccc ggcggaagag cctggatctc cctccagtgc ttgcgaaaat ccaggtcctg
     1441 gttccccacc tgcaccacgg tcctgagccc gagcgcgacc attccgtcga tgctcttctt
     1501 cggaagcgtc atcgcgaacg tggccatgtc cttgcccggc tcccagacct ccatccggac
     1561 cttcgcgtcc ttgccggccc accaggggtt ggccgaaggg ggctgggtcc ggagggtcca
     1621 gatcacgccg atccccaggc atacgaggaa gagcaggctc acgccgagga tcgtctgctt
     1681 gcgcgtcacg gggcctcctg agcagggcga aagggctctc taaagccgaa tacgcatcag
     1741 gcggcgggcc ggttccaccc ctttgtcacc gggtgccggt gaaccggatt ccctccgacg
     1801 gaggctccgc cccggtcgcc cgatcgtcaa cgtatttcat gcgcacggac agggaaacga
     1861 cggccacggt ggctccgaag aggcagacga tcgttgcccc catcggccag tccttgaggt
     1921 aggaaagccc cgcgccgagc atggatgcga caaaggcgat cgcccaggcc caaatgaggc
     1981 gggatgcccc cctccggccg agcagcgtcg aacagactac cggaacgatg aggaacgaga
     2041 aaaccgccag aacaccggac actcgcacgg ccgtcgtgac caccagaccg aaggaagcgt
     2101 agaagaggaa gtcccacttc ttcaccgata gccccattct tctcgcggct ttggggtctt
     2161 cggagcagag cagcagcttc cgccgcgcca ggaacaggaa gaggccaatg gccgcgaaca
     2221 gcgtggccat gatggcgatg tctttccagg tgacccaacc cagcgcctcc ccgctcaggg
     2281 ccccgctcac gtgctccgcc ccatgggggg cattcgaaag aaggagcagc gtggaggccg
     2341 tggccagggc gaatgagatt ccgatgaacg cctcctgcag gacggaatgt tcgcgatccc
     2401 agaggaagct gaacagcgcc gctccgagga gcgtgaaccc gaacgaccac aagtaggtgc
     2461 ttggatcgtg cggctccgcc cccatgagga acgcgacgct ttgacccagc gcggcgatct
     2521 gagcgagcgc caggtccacg aaaatcacgc cccgggccag cacgtgctgg cccagggagg
     2581 gaaggatcaa ggcgaggagc gcgcacgcga ggagcggcaa tgccatcacg ctgagaaacg
     2641 tcatcgtgat cctccctggt tgccggacag ggcccgggta agcgacgaga caatgaagtc
     2701 gaacttggaa aagacgtcat ccgttccggg tacgcctcct gcctcggtgg gcacctccac
     2761 ggcgacgcct ccggctcgtt tcgcgagctg cttggccgcg tccgcgttgt agtacggctg
     2821 gtaggtcact actttgacgc ccgatttctt catgtcttcc gagagctggg cgatgtagtg
     2881 cgcgttgggc ggaatgccgg gtttgggctc gacggtgccg aagagcttga gcccgaaacg
     2941 atttgcgaaa tagatgaagt tcaggtggta cggcacgatc gtcgcgccac ggtacggcgc
     3001 catctccgtc tcccacttct tcagtgaggt ctccatccgt tcgtcgaatg ccttcgcgtt
     3061 cgccgcgtac tcggccgcgt tggccggatc cagtccggag aagacgttgg ccaagtgatc
     3121 ggcgaccact tcgagcgcct tgggatccag gtagtagtga gggttgccga ggggatggat
     3181 atcgcccgcc gcccgactga cggtgccggt aggcttttcc agcacggaga cgccgcgcga
     3241 cgcatcgacg aatcccttgg cgccttgctg gatcttggga ttgcggctcc ccggtaagac
     3301 gagcgggagc caaccgccat cgagctcgag gccggtggaa acgtagacgt cggcgtgttg
     3361 aatcgccttc atgaggctcg gctttgcggg gacgaagtgc ggatcctcga acccccggca
     3421 gaggctctgg gcggtcacgt gcttcccgcc cacgaactgc gcgaccgagg ccaggtccgt
     3481 cagcgaggtc gcgaccttga ggtccgccca ggcgggcgac gcgagcccca gactgaggct
     3541 gagccacagg gcaagtcgaa cgatagactt catagattcc tccagcgtgt gtgacggttc
     3601 gttgatggat ctctagtagg catgggccgg gtggaacccg atggtgtagc tcatctggac
     3661 catgatgcgg tcgtcggtgg gatggatgcc gcgatccgcc ttggcgtggc tgtattccag
     3721 ccggatgaat gagaactccg atggcgtcca ggcgatgttc accgatcctc ggctgacgtt
     3781 gccggcgatc ggatcacctg ctccgtcgac catgaaatcg gtaacgctgc ggcgcgcccg
     3841 ctccccgcgg atcccacccc accagacctg cgacaaccgg tactggaacg aggcataacc
     3901 tccgtccgct tcccggtgat acgaacctcc ggggtaggac gccttctgga tgtactcccc
     3961 ctggaggatc cagcctttcc ggttcgagct gcgcgccggc acgttccgga tggtggcgtc
     4021 cgcgccgtag gcggcgtggc gatgtccatc ggaaccccgc ccctcgagaa tcgactggcc
     4081 cagctccatc gtcgtctcgt cgttcaggtc gatctggctc ttgaaatgac cgagcagcgg
     4141 gatgttgtcg tgccgatcgg agccgaggtc cagcggatgg tcatcatcga caccgacggc
     4201 cttgtaggcc ccgccggtga gctccgcgta ccagggaagg ggcgtcaacc acgatgcctc
     4261 gagtccggga tccttgaatc cctcctcgcc gatcgtgttc cccatcatga tcggcgcctg
     4321 aatgaagggg aaggcgtggg tgtgaaggag cccgtgcttt ccgaacgtcc cgcgaagctg
     4381 acccagcttg agctgaatcg aggtgagact cgtgttacgg acccacactt cttcgggatc
     4441 gatcgtgtga tccggaagga acacgatgtt gctcgtgaac gtccaatagg gatccacgac
     4501 cgaaagaagg ctgatctcgg cttcgtcgaa gtcgagcccg taggcgcgcg gcagattgtg
     4561 tgcggcttgc acggaaaaga gcgcgttcat cccgatcgcc ggattggcga gattcgagaa
     4621 catggaagtg gatgccccgg cttcggaggc ggacgccccg gcttcaggca ccacctgggt
     4681 actgtcgccc tcctctgccg atgcaacgct cgcgaacgtg tcgatgttcg cgaacgagga
     4741 caacatgagt acgctgatca gcgcttgtcg cagcatgacg gccgaatcct ccagtcgaat
     4801 gcggcaacgg ccccatggcg agcatgggac ctcgggccgg gcggcgcctc gtcaggcgag
     4861 ccggccgccc tcgtcgtcga aacggtcttc ggggactgcg ggaacgatca gcctaggggc
     4921 gggccgcgta cctgcgtgga agggaggctg ggtgcggagg gttgctcgat ccttgccgag
     4981 gagacgcgct cgtagcgttc gatcggcgga ggagagtcgg gaggacggcc catcgccacc
     5041 gtgccgctgt gcagcagctt gcagatctcg caggtgaggt cttcccggtg ctgatggaag
     5101 acgcccgtgg ctacccagca cagcagcagt gcaagagcaa ccagggctat acggcggagg
     5161 tcggctcgga taagagtcat caggcgttca accgtaccaa acgactcatg gttccgagac
     5221 aactttttgg gagtgtacgc cgggaccacc cagccggtgt cgtgctgcgt gcacccgttt
     5281 caacccttcc gcgcggccca gatcattccg atcccccaga cgatgaggag caccggccag
     5341 tagcggagga tgttgccgtg cagatatccc aattccttga gcagcagcac gatgccgatg
     5401 gtgatcaggg tgagggggaa cgcgattccg gtgccgccgc ctttgccggg catcaagtct
     5461 ccttggatat cgtcacgata gggtcggggt caaagcgccc ggacgggctc gcgcttcacc
     5521 ttgaggtgga tcagcggtcc ggccacgcgc acgtgcacca ccgggccgct tccttccgac
     5581 gtccagatct tgaccttgga tcgccccacc gcgtgctcca cgaaatcgtc gggaagggta
     5641 cggaaggtga gcgggtcgaa ttccagccgc gccgggcagc tcgcgggcag gtccagctcc
     5701 acgttcgcga gcgtcccgcg gaggagcacc agggaggggc gcgccgggcc ggtggcgtcg
     5761 atccggaccg acgacgcgat cgcctccacc tcgcagcgtt ccggccggag ccgggcgagc
     5821 gcagcgcgca cctgcgagaa gaggctcttg gccaggaggc ggacccggaa tcgctgcggc
     5881 acgagcacgc ggacgttgcc gcccagcggc gcctggtaga cccgcgcgtc ctcggtcggc
     5941 cagtcgaaga tcgcggcgcc gcccgaggtg gtgaagtccg cgtcatcgga gggcatgccg
     6001 gtggcgatca cgtccatgcg gcccgcccgg gacgccgcgt tcggctcgag agagagcgat
     6061 ccgccgaaca cccgcgcgcg taccgtgagc gtctccgttc gaggccgcgt gagatcgtag
     6121 gtcttggccg tctccgcgcg cgtgcccgtg ccgtgctgat ggacccacca ggcgccgaag
     6181 cccagggccc cgagcaccag gatcgtcgcg atccagccga tgagcggatt ccggagcgcg
     6241 ctggccagga gtgccaggcc gatcagcacg agaagggcgg gccagtagcg gagcagccag
     6301 ccgcccgtat cggtggggag atagccgaga tggccggcca agaggatcac ccccaggccg
     6361 gcgagaagca cgccgaagac gagcttcgcg aatcccacgg gaggaagcgg taccacgcgc
     6421 ggctccgggt tgcaagcgcg cgcgcggggt gctacgcggc cttgcctcgt gctggcgtgc
     6481 cgtcagcgag gcacctccgg aatcgccgaa gccgaatcgg cgggcacgag cgcgagcagc
     6541 gtgtcccaat ccgacggcgt catcgcgaac ctcttccgca tccgctcgac gcccttcacc
     6601 cagcgctccc gcgagtactt ccccggatcg ggcagcgcgt ggcagccctg gcaccggcgc
     6661 tccagaaggc gccaggcata gccctcctcc gtgacggcca cgcgttcctg gggcgttcgg
     6721 ggcgcgtgag cacagcccgc cagcaccagc gccacgagcg gaacccagcg cctcaaagct
     6781 ccacgccgat gatggcgcga acctgcgtgc gctcgaagtc gtccaggttc agatcgcccg
     6841 aggtgcgcgg gctgccccgg agctggcgca gcaccccgac gccgatctgg acctcgcccg
     6901 cctgcacgtt gaacacgggt ccgagcgaga gcgccgagcc cgaccgcggg ccgaagttcg
     6961 gatattccgc gacgtagcgc gcttccaccc ccgccgagaa tcggggcgag agctcgtacg
     7021 ccgtcccccc gacgaccttc caggcccatg ccttccgcat cacggcgccg ctctcgagca
     7081 gctcctcgtc gttgtgccgg aattcgaatt cgccgacgag attcatggcg ccgacgaaac
     7141 ggcgcgaccg gtgcgcgagc aggagcttgg gctccagctc cagctcttcg cccgattcct
     7201 tcgcctccag gtaaagcgcc gggtggccgg ggatccgtcc gggatcggcc agggcgtaga
     7261 tgaactcgag cgagggcgcc tcgaaatgga gggtcccgcc gccgggctgg tcgaagttca
     7321 gatacgccgc cgcggtgagc cgatcggtaa ccgagtactc gaattcctgg cgccactccc
     7381 aggccgtcaa gccttcgtcc tcttccttcc ccgaccggga ggtgagccag gtctccatct
     7441 cgatctcgcc ggcgggcgcc agatacggcg tgtaggtctc gacgaaatat ttgcgatccg
     7501 cgcgcgcggc tccgggaagg atcgccggaa tcagggccag cgcgaacacc accgcgacca
     7561 cccggcgcat cacccgctca tttcgtgtcg gcactcgttc ctccttggtt acggtcgtcg
     7621 gcttcgtggg cgcaatagag tcgcaggggt ccgtgcaccg agcaggtgga gcacgactcc
     7681 cccgaggcac agctgcggcg gtattcggcg aaggacgcca ggagctgctc ctggccgtgg
     7741 gtgtccgcgg tgaagcggat gaagtcgagg atgcggtcgg ccacgtcgtg gctcaccacg
     7801 tgctcccagc ggcaggcctc ggccgtggcg agcggaaccg ggagccccag cacttccgtg
     7861 agaaacgtga gcacgatctg gaagcggccg cgcacgtcga gggcgactct ccggcccgcg
     7921 gtcgtgagga gcgggaagtg ggcggcgtcg aagcgcacca ggccgcgctc cgccaggtgc
     7981 gcgagcgcga ccgagaccga gcccttggtg agccccatgc gcgcggcgat gtccacgacg
     8041 cgcgcgtacc cgcgctcctc gcgaagctcg aggatcgttc gaaggtagtg ttcgctgctg
     8101 accgacaggc ccgggcgttg ccgaaccttc gcggatgtca tgcgcggctc ctgtttgttc
     8161 atgccaacct aggtttgagc agccaaacct acgccgcggc gcccgtcgag atcaacgcaa
     8221 ttcttggagc gttccgcgcg catgaaaagg ggcccccaag gcttcggggg cccccgccgc
     8281 accgcgtggc tggcgtccat cgcggcgcgg acctgaacat cccaacggcg ctcagcgcat
     8341 cacgaccttg cgctgttccc gataggaggc ggaggcagcc tccacgcgaa cgaagtagac
     8401 gcccgccccg acggatcttc ccgccgggtc ggttccatcc cagcggaacg acgcgccccc
     8461 gttcgtgaat tcgagcccgg cccgatcaaa gcgggccacc gtgcgtccct gcacgtcgaa
     8521 gatccgcgcg acgaggtcgg gaggcgtcgt ttccggcgtc gctccggcaa gctgaatccg
     8581 gaacgcctcg ccgcgacggg tcggcgtggg ggtcgtcatc agcttccatc gcggcgcggc
     8641 cggcgcggcg ggtgccgcga tggcaggcgt gaccgcggcg tccgccagca tggtcggcgg
     8701 cacgtcgtcg ctcagcgatt tcaggtactc caccaggtcg ctcttctccg actcgctcag
     8761 cccaagcccg aaaaaggtgt tgtagtggtt caccaccgcc agcagcgtcg ggaagcggcc
     8821 gtcgtggaag aagcccccct tcgcgtgcgt ccagagcccc ttcaggggtg aggtccggta
     8881 gcgatggtcg ggcgcgcggt cggcctggaa gttgtcgatc ccgatctcct ccccgcgatg
     8941 catgttccat cccggctcgg tgtagagctc gtcggtgtga caggtggcgc acttcgcttt
     9001 tcccgtgaag agaacgtcgc cgcgcgccgc cgcggcctgg tcgaagctgc ccggcggcgg
     9061 cttcggcgcc gggagcgaga gctggtagaa atgcagcgcc ggaagcttgg gagtgatccg
     9121 gtcgtccgtc gtcgttacgt ggccgaagtg gtgcgccgcc gcgatcggga actgagatgc
     9181 gtcgtccagc cgcgggtcga agaaggtccc cgacccgtgc atctccagat tcgcgacgaa
     9241 ggcgttccag tagggcaccg agccccagcc ggtccaggtg tgcaggttca cgccggccag
     9301 accaaaggcc ggcgggatga gcgtggccgc ggaccgcccg tcgggctgga acgccttccc
     9361 atccaggatc agctcggcgt cgaacttgcc cgggccccag ctgaggagga cgcgccggac
     9421 ggtatccacg tcgacgccca ggaggtcggc gatcggctgg aggttcggcg ccagcgacac
     9481 gatcttcccc acgttcagat cgcggttggc ccagccgtcc aggcggtgcc cgattcctgg
     9541 cgcgagcgag ttgtccacgg tggaatggca gaacgcgcac tggatcccca tcgtctggag
     9601 atgcccgtcg ccgcccacga cgcccgtgac ccccacgacc gcgttctcgc ggagcagctc
     9661 gatcgtcacg gccgggtcgt ccagattgac ctgcccggcg gcgatcgcgt cgcgcacggt
     9721 gggggagagc gcgtcgacat ccaccttgag gccgacgtcg agggcggtcc tggggctcac
     9781 gcccggtccc acgccaccca gggccgatcc ttcgatcgcc tcgtgcagcc ggagggtccc
     9841 gccccagtac gcctcatccc cgaaggtgtc atagcggaag gtctggcgcc cctcctccat
     9901 catcgcgttt gcgtgctggc ggacgatctg gtcgaagttg gcgccgggca gatggggctt
     9961 tggccgcttg acgctttccg cggcctgggg cgcggcgacg accaggtaca gggccgcaag
    10021 cgccgcggag accgcgatcc atgaacgaag tctcggcgac atgatgaaca cctcctcagc
    10081 ggtgcctgga tccgaacggc gtggaggcac gtccgatgac ggaaacgcgg tcatgcaccg
    10141 cggtcgtctt cgtattagtc ccggcacggc atcgttcgtt acagcgccgg cgttcaattg
    10201 gccggacatg gggagtacac ttttcggggt atggaccgga tggatggaaa accgaccttg
    10261 ccggacggtg ccagtccgga aggcccactc ctcgcgaagc ttcgaggggg cgatgaggcc
    10321 gccttcgccg cgctcgtcga cggcctgaac tgcaggcttc aggcgctggc cgccaccttc
    10381 acgtcatccc ccgctctcgc cgaggacatc gtgcaggaga cgtggctcgg cgtgatccgc
    10441 ggtctggacg ggttcgaagg ccggtcgtcc ctgcgcacct ggatcttcag catcctgatc
    10501 cgccgcgccc ggaccctcgc ggcgcgcgag gcccgccgcg ccgacgcggt gtcgacgaac
    10561 ggggccgcgg atcacgagga atgggacgtg ggacggggac gggtcggatt gtgggagggc
    10621 gcgcccgagc cgtgggctct cgacacgccc gagcaggtcc tccaggggcg ggaagcgctg
    10681 gagatcgtgc gcacggccct ggatggactg ccggagtcgc agcggcaggt ggtgctgctg
    10741 cgcgacgtcg aggacgtgtc cccaccggat atctgtaata tcctggactt gaccgagacc
    10801 aacatgagag tgctccttca ccgcggccgc gcgcgcatcc gtcgcgcgct ggacctgtat
    10861 ctccgcgaag ggacgcgccc gtccaccgcc ccgaccccgg tccaggagac tcgatgaact
    10921 gcaaagaggc gctcgacgtc gtctcggaag cgctggacga aacgctcgag gagagcgccc
    10981 ggcgggagtt cctcgagcac atggaccggt gttcggcctg ccggagctac gtggagcagc
    11041 tccgcatctc ggtccaggcg ctgagaagcc ttcccaagag cggacggccg aacctgcgcc
    11101 gcgcggagat catcgaagcc taccgcaaga aattccgctg agccgcggcc gcaccccgcg
    11161 ccccgtggcg gctcggggct tttgcttgac attcgcggct tcgccggaca gaatctgcgc
    11221 ccattcaagg agttaggagg ggcgcatggg cgagctcacc gggaagaagg cgctggtcac
    11281 gggcgggacg cgcggcatcg ggagagcggt cgcgctggac ttggccgaca tgggcgccga
    11341 cgtcgcgatc aacttcttcc gcagccgcga gtcggcgaag gcggcgaccg ccgagatcga
    11401 atcgcgcggg gtccgcgcgc tggcgctccg cgcgaacatc gggaacgagg aacagatccc
    11461 cgcgatgttc gagcagctga aggcggagtt cggcaccctc gacattctcg tctccaacgc
    11521 cgcgctcggc cacttcggca acgtcctcga cgtggacgac aagatgtggg atatcgcgat
    11581 gaacaccaac gccaaggcgc tcctcttctc ggcgcagcag gccgtgaaga tgatgcccga
    11641 gggggggaag atcgtcgcgc tcacctcgct cggcagccac cgctacatcc ccggctacgc
    11701 gtcgatcggc gtctccaagg cggcgatcga gaccctggtg cgctacctgg cctacgagct
    11761 ggcgccgaag aagatcaacg tgaacgcggt ctcgggcggc ttcatcgaca ccgactccct
    11821 caagatcttc ccgagctacg aggagatcat cgtggagagc acgcgccgca cgccgttcgg
    11881 ccgcgtgggg acgcccaagg aagtggccaa catggtcgct ttcctgtgca ccgacaaggc
    11941 gtcgtgggtg acgggccaga cgatcatcgt ggacggaggc tacacgctgg gttgagccac
    12001 gcccgattca ccgtcgcggc gaccaccatc cggtgagcgg cggactcccc aggaacgccg
    12061 tcagcctccc gtcgaaaagc aagaacccca cgatgcccag gttcacgaag ctcccggtga
    12121 tcaccgtgcg cacgagcgga acggttcccc cgaactcggg cggtctggtg atgagatacg
    12181 cgaggagcgt cagctcgctc acgagggcaa cgacgatccc ccacggacgc gccgcgcgct
    12241 ttcccagcca gatccccgcc atcacgtaga acgatcccac cgcgagcagc gtcgagaaga
    12301 agatgctctg gaaggcggtc tgcagcgcgt accccgcgag cctcccctcc gcggactggg
    12361 ccgccacgaa gaacgaggtt ccgatcacgc cgctcaaaag ctcctggaac cccttgatcg
    12421 cccagagcat gaccatggcg taaacctgcc acgggcggcg caggcggcgt ggcacgtcgg
    12481 gctcgggaga atcgagcggg cgaagcgtgg tcacaccggc gccgccacga tgatcgtcgg
    12541 ctcgtgccgg acggggaagt tgaccgacgc ggcgatgaag cactccgtgt gcgcgcggtg
    12601 atggagatcg cgcgcgagcg ccgcgtcgga atcgggcgcg atcgtgacgc gcgggcggag
    12661 gagcacgtcc gtgaagtgaa aggtcttctc gccgcgcgtc atgatgccga cggcgtcgtc
    12721 ctcataggcg gtcacccgca cgcccttccg ggcgcagagc gcggcatacg acaggaagtg
    12781 gcacgaggcg agcgcggcca cgagcaggtc ctcgggatcg gcgtacgccg ggtcgccgcg
    12841 gaataccgcg gcggacgtca tcgtgagtgg cggcttgccg tcaaattcga cgcgatgctc
    12901 gcgcgagtag gtggcgtagt ccacggtggg gcctttcgag gcgcccgtcc agagtaggtg
    12961 gcactcgaat ttgtgctcag cctgcgaggg attcatcgaa actcctttcc ggcgccctgc
    13021 gggcgccggg acatgatggc aggtcgcgcg gtcgcggcct cagggctctg gtccagggcg
    13081 ggatcgatgc ggcgccggag atcggcgtag ccgatcgcgg cgagcaccgt gaggctcgcg
    13141 atcgcgctcc agaggagctt gggcctgccg gccgccagat cgagcagcac gccgcccacg
    13201 aggggcccga ccgaccagcc gaacgagttg aagattccgt acacgcccat gtagcggccc
    13261 cgctggtcct cgggggagaa gttcgcgacc aggttcatcg aggccggcga gcaggtgatc
    13321 tctcccgcgg tcaccacgaa catgcccgcg agcaggagcg cgaaactcga tccccatccc
    13381 atcatcccat acccgatcga gtagagcagg cagcccacga tgagcgccga cgtcatccgt
    13441 agcggcgcca ggatgcgcgt caccgggaac tgcagcagca cgaccatgac gccgttcagc
    13501 gtgtagatgg cccccagctc caccttcgtc cgtcccgccc agtccaccgc gtagaccgag
    13561 aaggtcgcca tgagctgccc catgaccgtg aagagaagaa gcgtgaccag gcagtagcgc
    13621 aggaagagcg tgtcgcggag cacggcgagc gttccggcca tcgagaggcc gcgcccggcg
    13681 ccgacgggtc cgaccggcgg gatcgcgacc cgctccgggc cggcggcatc ctcccggcgg
    13741 cggcgacgcc gcgggtgggt ctcctccacc aggaccgcga tcaggatccc ggccgtcagc
    13801 agcacgcccg acgcgacgta gaagagcgac gcataggaga gcgcggcgag gaagccgccg
    13861 agggcggggc cgaccgccca cccgagattg cctccgatcc ggagcacgcc atacgcctcg
    13921 acgcggcgct cgcccgacgt gatgtcggcg atcatcgcgc cggagggagg ctcgaacatc
    13981 cgtccggcga acgcgctcag ccctgtgagc agcgcaaaca tcacgatcgg ggcgtggacg
    14041 gtgacggcgt agcccagccc cgcgaaggtg accgatcgga tgacctgaca ggtgaccaga
    14101 acgcgttttc gcccgctccg gtcgctccat tcgccgccca cgacctgccc ggcgaccccg
    14161 cagagcgcgc cgaagaagaa aagcgatccc accgcgctca tcgacacgcc gcgcgtgccg
    14221 tgaaggtaga gggcgagaaa ggggatgacg accgagaagc cggtcgaggc gaggaagcgt
    14281 cccaggaaca gatggaggag ccgccggtcc ttgtcgaaca gggcgcggcg cgggagcacc
    14341 gtatcgatcc agcggagcat ggggcgcgac gcgttccgga gacggggcat gggtcgtcgt
    14401 tcctgctgct tcgtttccaa tggatggagc ctagcacacc gggtaggctc cgcgcccatg
    14461 accccgcgcg acgataccgg cgtacggcgg acccggccca atccagaggt ccgcatggac
    14521 gacctcggtc cctggcgtcc ggccccgcgc gagggcgcgc ccgggcacta ccccgcccaa
    14581 gacaaggtga tgccgctcgc ggatgcggtg cggcggttcg tgcttccagg agccacggtc
    14641 gcgctcggaa cctgcctgga gcagatgatc ccctttgccg ccacgcgtga gctgatccgc
    14701 gcagggatcg gcgacctcac gctcgtcgga ccggtttccg acatctgctt cgaccagctg
    14761 atcggggcgg ggctcgcgcg gcgcgtaatg gccgcgtggg tggggaacgt gatgatgggc
    14821 tcggcgtacg cgttccggcg cgccgccgag cgcggggagc cgcgcccgat cgaggtcgtg
    14881 gacttctcga acttcaccct cgcgctggcg ctccatgccg cggccatcgg cgcgcccttc
    14941 attccgacac gcagcgcgct gggcacggac tggctggatc gcaatccgtg gctcctgcgg
    15001 gtggaggacc tggtgaacgg agggccggtg ctcgcggtcc gcccgctggc gccggacgtc
    15061 acgatcatcc atgcccagcg cgccgacccg cacgggaatg cggtgatgtg ggggagcctg
    15121 ggcatcacgc ccgacgcggc gcgcgcggcg cgcgccgtga tcgtcacggc cgaggagatc
    15181 gtgagccccg agcgtatccg gagcgatccc aaccgcgtcg tcgtcccggg gctgttggtc
    15241 gcggccgtct gcgaggcgcg ctggggcgcc cacccctcgc ccgtccaggg ctactaccgc
    15301 cgcgaccacg aggcctacgc cgcctaccac gagcgcacca agaccgccga gggattcgcg
    15361 gcctggcgcg cggagtgggt ggacggcgcc cccgacctcg cggcctacgt ggcccgcgtc
    15421 gggaacgagc ggatccattc gctgatcccg ctggagcacc gcttcgccga tccggtggat
    15481 tatggatact gaccgatcct cctcggcact cctcccggcg accccggagc ttcgcgcgaa
    15541 ggcacaagtc cggacgcttc ccgagtggct gaccccgatg cgcgcgctcg cggccgtcga
    15601 ggacgctccg catacgatcc tgttcgagag cgccggcgcc ccgggcgcat cctcggagtg
    15661 gaccctgctc gcgttcgatc cgccctggcg cctcgaagtg gccgaaggcg cgctctggga
    15721 ggtgcgcggg gagcggcgcg cgcggaggcc gggcgctccg ctggaggcgc tcgcggcggc
    15781 gtggccttgc cgcgcggaga tcgaggacgc gccgtccgtc ccgtttctct ccgggctcgc
    15841 gggggcgatc gcgtacgact tcaaggactg gatcgagcgc tatcccgacc gcgcccggcg
    15901 cgagcgcccg acccccgatc tctcgctcgg cttctacgac tgcgtgtggg cctggagccg
    15961 cgcgaccgga gaagggtggt gcgtctcgac cgggcttgcc gagaacgatc ccggtcggcg
    16021 ggaacgccgc gcgggcgagg tgctggaggc gcagtggtcg cgcgtgacgg ctgggggcgt
    16081 cgcggatggc tccggtctgg gatccgtggt ccgcgcgggg gcgatccgct ccaacttcac
    16141 gcgcgacgcc tactgccgca tggtcgaggt cgcgctcgaa cacatcgcgg cgggcgacat
    16201 ctatcaggtc aacctgtcgc agcgcttcct ggccgattcc gcgccgcggc cggcaacgct
    16261 ctaccgctcg ctccgggagg cggcaccggc gcccttcatg gcgtacctgt cgctcgagga
    16321 gggaggggtc gcctcgtcgt cccccgagcg gttcttccgc gtgcgggggc gccacatcga
    16381 gacctggccg atcaagggga cgcgtccccg cggggcgacg ccggaggagg acacagcact
    16441 ccggctggag ctgatccaca gcgacaagga ccgcgccgag aacgtcatga tcgtggatct
    16501 cgagcggaac gacctcgggc gcgtctgcgc gatcgggagc gttgccgtgc ccacgctctg
    16561 tgaggtcgcg agccactcca atgtccatca tttcgagtcg cgagtcgtgg ggaccctgcg
    16621 cgaggacgcc gcacccgtgg acgtgctgcg cgccctgttc ccgggaggct cgatcaccgg
    16681 cacgcccaag atccggagcg ttcagatcat cgaccggctg gagccggtgc ggcggggcat
    16741 ctacacgggc gcccttggct actgggacgt ccgcggcgac ggcgactgga acatcgcgat
    16801 ccgcacgatc gtggccggcg gaggcacggc gtcgttccac gtcgggggcg gcatcgtggc
    16861 cgactcgacg cccgaagggg agtacgagga gacgctcgtc aaggcgagcg ggatgatgcg
    16921 ggcgctcggg gtgcgcgctc cgtcgccgtc tacgtcgagc ccacgcgcgt agcgcggacc
    16981 cgccgcaaca gcacccgcag ggacgcggga tcgatcggct tcaccacgtg ttcgtcgaag
    17041 cccgcttcct tcgagcggcg gcgatcctca tcctgtcccc acccggtcag cgcaaccagc
    17101 cggatctcgg cgccccacgg ctccttccgc agccggcgcg ccacgtcgta tccgttcatc
    17161 atcggcaggc cgatgtcgag gaggatcacc tgcggacgga aagtctcggc ctcgcgcagc
    17221 gcctccatcc cgccgttggc gatgcgcacg tcgtagccgt ccagggcgag caggcgcccc
    17281 aggctgtcgg cggcgtccac gttgtcgtcc acgaccagga tgcgcgccct ggcgtcgcgg
    17341 gcgcccggac cctccgtggc ggcgggcggc gggagcggtg gcggctccgt cgtcgcgagc
    17401 ggcaggcgca ccaggaactc gctcccccgg ccggccccgt cgcttcgggc ctcgatcgag
    17461 cccccgtgga gctccacgag cgttcgcgcc agcgtcagtc cgatgcccag gccgccggcg
    17521 gtgcgctcca gggaggaatc gacctgggcg aacatctcga agatgcggcc cagcatctcc
    17581 cggggaatcc ccacgccgtt gtccttcacg cggagcacgg cctggtcccc ctcgcgctcg
    17641 gcgcccacct cgatgcggcc gctgggcggc gtgtacttcg ccgcgttggt gagcaggttc
    17701 gagaccacct gcgcgatccg cgtcaggtcc ccgttcacgt agagcggctc tcccggcagc
    17761 gagacggtca gcgcgtgctg gttcccctcg atgagcggcc gcgccgtctc gacggcgctg
    17821 ctcacgatga ccgaaaccgg gatccgctcg cggcgcagct cgatcttccc gcgcgtgatc
    17881 cgggagacgt cgaggagatc gtcaaccagg cgggagagat gctcggcctg ccgttcgatc
    17941 acctggcgcg cccataccag ctccggatcg cgcggcccgt gctgtttcag gatgtcggcc
    18001 gcgttccgga tcggggcgag cggattgcgc agctcgtgcg ccagggtcgc gaggaactcg
    18061 tccttcatgc gccccgactc cttgagctcg cggacgtggc ggatccgctc gtaggccagc
    18121 gtgacgtgct ggcagatcgt ctcggcgaac tccagctcgt cgggctcgaa ggtgtcgcgc
    18181 gagcggctgg ccagcgtgag cgtgccgatc aggcggtctc ccgcggcgag cgggtggcag
    18241 atgagggcgc gaagtcccag ggcccgggcc acgccgtctt cgccgtcgct cgactcctga
    18301 acgcgggtcc gcacgatggg atggcgcgtc acggcgacct tgccgttcac cccctggccc
    18361 agaggcacgc ggtccgcctc gcgcatgacg tcgggcggag cgccttcgta ggaggccagc
    18421 agaagcgtcg tgcccgactc gtccagcatg tagttccagt agccgtccag ggccagctcc
    18481 ggccggatct tggcgaacag ggcgtggagc atcgcatcgg gatcgtcgct ggtgagcgtg
    18541 acggccgcgg cctcgaagag caggcgaaga cgccggtttc gcgtgcgcag ggccacctcg
    18601 gcccgtttcc gctcggtgat gtcgatgccg atacccaccc gcgtgtcgtc gctcaggcgg
    18661 atgttggccc acgaggtctc gatcgtgcgc ccgtcgcgcg tgcgcatgcg cacgtccatc
    18721 cacccctccc ggcaggactc catgaagtcc agcacctgcc ggcgataggc gggatcggga
    18781 tagatctcct ccatgagcga gacgcccttc gcctcctcgg tggaccagcc gacgacccgc
    18841 gtgaattccg gattcagacg cagcatcgtg gcctcggggt cgaacatcgt gatcatgacg
    18901 gggatggtgt cgatgatgcg gcgcaacagc tcgcgctcgt gccgcagcga ctcggccgca
    18961 cgccaggtct ccgtctggtc gatcgtggcc gcgacgatgc ggatggggcg tccctggcgg
    19021 tcgacgcgaa cgaccccgtg cccggacaga cggagaacga cgccgtcggg ccgggtgtag
    19081 cgggcctcgg cggcgaaagg cttcccgttg acgatggcgc gctcgagcgc ggcctggatc
    19141 gcggggcggt cctccgggtg aaccgaggcg agtccttcct cgatgctgcc gaagcgcgag
    19201 gtgccgagca tccggcgcgc ttcgggggag gcgtggatct tccccgtgcg aaggtcccag
    19261 tcccaggttc cgatctggcc cgctcccagc gcgagctgga gctgctcgtc ggtgtcgtgc
    19321 cgctgccgct cggcgcgatt cgcgcgctcg ccgaagaaga tgatcaggga gcaggagacc
    19381 agatagagcg tgaagccgac cagcgaggcg gggtcgccga tgtggaagga gccccggggc
    19441 tggatgaaca ggtcgttcgc caggacatag ccggcgagcg tcgtgatgac ggcgtaccga
    19501 agccccgcga accagaccgt gatcgccacg gccccgtaga gcgtgatgag cgccagcttg
    19561 tcgtcgagga tcgggtccag gagggaccgg agcaagatgg ccgcagcgac cgacgcgatc
    19621 gcgatggtga gtccggtaat ttcctggaag accttggctt tcatgctcct ccggttctca
    19681 gcgcggccct gagcttacca tcggagcggc cccggagtat cgtggcacca tgcgagcgat
    19741 gatcctgcct cagcaggcgc cgatcggatc cgcgcccctg gtcttgcgcg acgtccccga
    19801 gcccgaaccc gctccggggg aggtgcgggt ccgcgttcgg gcgtgcggca tgtgccgcac
    19861 cgatctccac gtgatcgagg gggacctccc cccgcgcaaa ctgccgctga tcccgggaca
    19921 ccaggtcgtg ggggtcatgg accgcgccgg cccgggcgcc gcccggttcc gggagggcga
    19981 ccggatcgga atcgcgtggc tccggcacac gtgcggcgtc tgcgtctact gccgctccgg
    20041 ccgcgagaac ctctgcgagt cctcccgttt cacgggctac cacgccgacg gcggctacgc
    20101 cgaggtcgcc gtggtgcccg aggccttcgc ctacgcgatc cccgacgtgt tcggcgacgc
    20161 cgaggcgacg ccccttctct gcgccggcat catcggctac cgggcgctgg agcggagcca
    20221 ggcgcccccg ggcggaacgc tggggttgtg gggcttcggc tcgagcgcgc acgtaacgat
    20281 ccaggtggcg cgcgcgcgcg gcatccaggt ctacgttgcc acgcgcggcg agaagcacca
    20341 ggcgctcgcg cgcgcgatgg gcgccgtcta ggtcggcggc gcgaccgacc ccctgccggt
    20401 ccccgtggac ggcgggatcc tcgtcgcgcc ggcgggggag atcgtcccga tcgcgctgcg
    20461 atcgattcag aagggaggga cgctcgcgat cgcgggaatc tggctgagcg acatcccgcc
    20521 gctgaactac gagcgcgagc tcttctacga gcgcaatctc cggagcgtca ccgcgaacac
    20581 gcgcgcagac ggagaggcgc tgctccggga agcggcggcg attccgctcc ggccgcgcgt
    20641 cacgaccttc cccctggagg aggcgaaccg cggcctggcg ctcttaaaga gcgacggtct
    20701 gaacggcacg ggggtgctgg tgccctgacc gcccgatgaa gaagggcggg ctccagtcgc
    20761 ggaacccgcc ccggtattcg cggaaacgac gcgcggccta gatgcccacg tcgatcacca
    20821 ggaccagctt cccgttttcc acgtcgaact gatacgtgcc cgcggaaccg acggcgtaga
    20881 gcgtgaagtt gccgtcgccc ttggcctcct ggagcaccgc ggccttgccg gccgccgtga
    20941 agagggccgc cgaggcatcc caatcgatcg tcttcgtgcc gttggccggg accttgaagg
    21001 ggccccagag cttgatgccc gtcgtcgtga tggtgagggc gtcggtcaag gtcgtgaggt
    21061 cgcgggtcat gtagacctcg acgttcacgt ccgacgtgcc cgtgttgtgg aacgtgccga
    21121 gcacggcgaa atcggagatg tccttgacgt tgtccttgtg gtccttgtag tcgctgtcgt
    21181 cgttcaggtc gatctgctcg cccacgatgc cggtcgagct ggtcgccgtg atgtccggca
    21241 gatcgaagtc gagcgtgaac tggcccgagg tcagaatgca gcccggcgcg aagagcacga
    21301 gcgccaagca gggcagaatc catcgcagat tcttcatcgc gcgcctcccg tcagggtaag
    21361 aggtcgtagt tcaagccgac cgtcgcgttg ccgaacttcc gcgacgtgtc gccggtcttg
    21421 atcatgttca cttcgccgcg gatctggagc gacaccttgg gcgtcggcga caggccgaac
    21481 cccagcccga agttgtaggc tgtttccttg atctccggcg ttccggtccg cgtcagcttg
    21541 tgggatccga tgccggcgaa gggatagaaa tggaagcccg cgccgccgac gtgtcccagg
    21601 atcgcgttgg cgccgtagcc cttgttatcg aagccgtcgc gcgtgtactc cacgccgccg
    21661 atcgtctccg tggcgtcgtc catcttgccg gtcagccagt agggctcgac cgtgatcatg
    21721 ggcaggagcc ggacgggcac gcggagtccg tacaccgatc cgttccccac gtcgtcctgg
    21781 agaatgggat agctgagacc cccgaaggct ccgacgccga cgccgaccgg cgcggcggag
    21841 gcgacaccgg cggccagggc gagcgcgagc actgcgagtg caaccgttcg catcatgacc
    21901 cctcctcgtg atgtgtgtga cccgatgcgc gggaggatag ggggggaccg attcgcttgt
    21961 ccagcgcgct aggtgcgcgt tcccgtccaa aagcccccgg gatcgatcgc gcgcagcgct
    22021 ccgatctcgt cctcggtaaa cgggggcgtg aggccgatcg gctgctggat gcgcggctcc
    22081 cagctcatgc cggccatcac gtggtcgatc gagagtcccg gatagacttc ggagaggaac
    22141 gcctcctcga tcgcggcgtc gaaccggaag accgcgtgcg tggtgacgat ggcgctggga
    22201 cccccgggcg ggaggccctg cgcggcgcgc cagcccgggc cgtcgccgcg tccgggcgag
    22261 gtgcggaacg tgacgcgcgg gaccaggcgc cgtttctcct ggggcatcag gatcaccgtg
    22321 cggcgcgcga gcgcgatgat gtcggaggcc ccgccgcttc ccgggagccg cgtgatgccg
    22381 ccgtcccgtc ccggaacctc ggtcgagttc acgttgccga agcggtcgac ttcggcgccg
    22441 cccaagaggc cgaggtccac gcgccccgcc gcgagcattc ccatcacgtc gatcgtggtc
    22501 gacgccatgg tcgcgtccag gatgttcggc ggatcgccca tcgtgaagag gagctcgcgc
    22561 gcggcttcct cgcggatcag cccgttctcg tacagcgcca cggcgtgcgg agcgtgcgtg
    22621 cgcttcgcga ccagatacgc cagaaggggg agccgcgttc ccacgaacac ggtctccccc
    22681 tcctggatct cgcgcgccgc gcgcagcacc atccgctccc gcggagcgac cggcggcgtc
    22741 acggcgcgag aactacttgc cgctgaactt ggcggcgcgc ttctcgacgt aggcggtcag
    22801 gccttccttc gcgtcctcgc tctggaagag ctgctgctgc agctcgcgct cgatcgcgag
    22861 gccctcgccg aacgccgcct ccagccccga ctgcaccgac cgcttgatcc ggccgaccgc
    22921 cttcgaggcc ttgttgggcg gcgtgaactg ccgcgcgaac tccatgatct tggcgtcgaa
    22981 gccgtcggcg tcccagacgt ggttgaccag ccccatctgc tcggcctctt cgaaggagaa
    23041 ggtggagccc agcgccatca tctcgatggc gcgcggcttg ccgaccagcc ggcccaggcg
    23101 ctgcgtgccg cccgtgccgg ggagcacgcc cagcgtcacc tccgggagac cgatcttgcc
    23161 cgcgcccttc ttcgcgatcc ggatgtccgc cgccatcgcg atctccaggc cgccgccgac
    23221 gcagtggccg ttcagcgccg cgatcaccag cttgggcgtc tgctccaggc gattcagcgt
    23281 ctcgttggcg tggaggcaga agtagtactt gaagaccgga ttggcggtct ggagcatctt
    23341 gatgttcgcg ccggcgcaga agaatttgtc gcccgcgccg cggagaagga tgacgtgcac
    23401 gttctcgtcg aagcgcgcct tcaagatcgc ctcgtccagg gcgcgcatca tctcgtaggt
    23461 gtaggtgttg gccggcggat cgcacagctc gaggaccgcg atgccgctct cggtccggta
    23521 ggtgcagagg ccgctgggag cggccatggt ctcgggggtc acggttcggt cctcctggtt
    23581 cgcggggtca cggggaccgg cgggccgggc ctgccggcgc ctcggggtcc tgccgctctt
    23641 cgtcgatcgt gcccggagct tacccgcttc c
//
LOCUS       DASSLZ010000349         6908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_242063, whole genome shotgun sequence.
ACCESSION   DASSLZ010000349 DASSLZ010000000
VERSION     DASSLZ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242063"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2036
                     /locus_tag="VFM00_13175"
     CDS             <1..2036
                     /locus_tag="VFM00_13175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET7499244.1"
                     /translation="SARGSLQIHVNQGPNHDPIASVPTRITMVAGTVWRKTLVLRDLD
                     GRSLSFQKASGPDFLTAGVVGQGEGGAVGFLRLGPSSCDAGEYEASLTVGAGATAATR
                     SVHVSVRPAMAAPPSDLIELPTRLYPSGLAGGDLNGDGLMDLVVVNELHSLSVFVSLG
                     GGAFATVRSYPLGAYPIIPTSTTLADFDEDGRLDAAVGESGAIKVFRGRGDGSFEPPS
                     ALPAKVLPMDVIAADLNDDGHLDVAAADGGTSAVSLFLGRGDGTFAPRKDIPVPGTPY
                     GLTSGDWNRDGRVDLAVAGAFFSKVSILLGHGDGTFHDPTSLPAGRSPFSVATGDWDN
                     DGKLDLAVADFNGSLFIDRGDGAGGFAQTDEFDGFTAPQTVTAGDLDGDGIDDLVLAD
                     GGGSKAQVFRGDGAGGFVASETIALPGGAHHCAIGDVDGDAVPDLAMTDQRYGAALVR
                     RFASPQNAAGFARAFVTGDHRPVIIASRTPAFTVQVEPVAESFAIQDVDLESLRLASE
                     GTGSVASIGGILAKGASAGDRDHNGILELSLWFGGEDLARLFDRVTGRRDVPVQFEGR
                     LLSGRLFCTRLVLTVQGPGSPMTASVAPNPLNPSGVLRFVTTIRGPLHVALFDTQGRR
                     VRTLVETKDAPAGAQRIAIDGRTDSGSALASGIYFFRIEAAEGSTRGRVTILK"
     gene            2110..2760
                     /locus_tag="VFM00_13180"
     CDS             2110..2760
                     /locus_tag="VFM00_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499245.1"
                     /translation="MTPDDPATPPAAAPPARTLFLLSPANCGGRRAQILRSERAAFPL
                     AVQLREAGAPLGEVFSFLSGLYFRGKLAYGMAFGSAATRPGALVITPGRGLVPADTIV
                     RMDDLEEIASIGVSQDEPRFREPLERDAAALAATLDAGDRVVLLGSIATGKYVDVIER
                     LLDGRLFFPAAFVGRGDMSRGGLMLRCVLRGEELEYVPIAGAIRRGPRPPRLEPIR"
     gene            2757..3749
                     /gene="ligD"
                     /locus_tag="VFM00_13185"
     CDS             2757..3749
                     /gene="ligD"
                     /locus_tag="VFM00_13185"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET7499246.1"
                     /translation="MSPPRAPAERPAKGPVKRPAKPKAMAIPADVRDVELDFGGGKVV
                     RLTNLDKPFWPELGIAKRDLLQYYAGIAPVLLPHLRDRAMVMKRYPNGAHGQFFFMKR
                     APSPRPEWIETCSIPHDSGNVIDFPMIQDLPSLLWVINLGCIDLNPWYARCDDTDRPD
                     YLHFDLDPVPGASFDKVRETALLVRRGLEGLGMTCHAKTTGSKGIHVYIPIRRGPLQK
                     EVWGFAKSFAIALAAQAPALVTAEYRVAKRPAGRVLVDYNQNAWGRTLASIYSVRPRP
                     RAPVSTPVSWKEIERGIEIEDFRIDNVPQRVAKLGDLWKPLLGAKGRVALEKFL"
     gene            3779..4834
                     /locus_tag="VFM00_13190"
     CDS             3779..4834
                     /locus_tag="VFM00_13190"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET7499247.1"
                     /translation="MEARSVDVIPVGPEWEYEPKWDGFRCLAFRDGDLVALQSKAGEP
                     LERYFPEMVEALREVKAPRFVVDGEIVVPSGTSLSFDDLLMRIHPAESRVRKLAAETP
                     SMLIVFDLLVDEKGASLVALPLTERRPRLEAFAARFFENHRSIRLSPASADPRDAMRW
                     YEKTRGALDGVIAKRRDLPYRSGDRSGMVKIKQRRTADCVVGGFRYASKGKSEVGSLL
                     LGLYDDQGLLHHVGFTSSFSAPERKALVPKLQALVKPPGFTGNAPGGPSRWSTERSGE
                     WQPLQPKLVVEVEYDHVSGSRFRHGTKFLRWRPDKAPKQCTFEQIERPEGSLLQLLGA
                     GVRPKAARSARPSSRGS"
     gene            5050..5742
                     /locus_tag="VFM00_13195"
     CDS             5050..5742
                     /locus_tag="VFM00_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499248.1"
                     /translation="MHPIRVAAVAASVVLILAAPSLSRAHGDSHANDHGFAYGNHGDD
                     FTWAIVDGGNTSMSGQVDHPTLEELKERFGERFLYIEDDEDGYVITDRAMVERARRAT
                     ERIGRYGREIGEIARTQAKLSLSEARPKIRRAELLREQAELRREIDRAERQGEPTDRL
                     ERRLDRVTTRLEVEDQVYGGFALSSDEKRDLTRRRDEAKARLHEAVEQVNREMRDILR
                     DAKDRRIAEPVD"
     gene            5826..6362
                     /locus_tag="VFM00_13200"
     CDS             5826..6362
                     /locus_tag="VFM00_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7499249.1"
                     /translation="MHPPTVVLETPRLTLRRFTLEDAPFVFEMVNDPDWIRYIGDRGV
                     RTLDDARTYIRDRTLAQYDRVGFGMYVVALRHGGEVVGSCGLIRRDSLDDVDIGFAFL
                     ARHRGQGYATEAAAAVLDYGVRSVGLTRIVAIVASDNHRSIRILERIGMHFERMIRLP
                     GEDEEIPLYAYEAPGAAA"
     gene            complement(6368..6634)
                     /locus_tag="VFM00_13205"
     CDS             complement(6368..6634)
                     /locus_tag="VFM00_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HET7499250.1"
                     /translation="MDEACAHLKQIHDVKHPKLRQCDECVPIGARWVHLRTCQQCGTT
                     LCCDSSPNKHASKHAKAVHHPVVASAEPGERWLYCYPDESFVEY"
     gene            complement(6639..>6908)
                     /locus_tag="VFM00_13210"
     CDS             complement(6639..>6908)
                     /locus_tag="VFM00_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HET7499251.1"
                     /translation="ISHGFWPGSGAVPDAAFYAYAAPAPAGFEAAAVEPAAASYNREF
                     GEFILPFEAVRQSADPVAALTAFLNSTYEAGARLAGWDRDELERR"
BASE COUNT         1032 a         2434 c         2392 g         1050 t
ORIGIN      
        1 tgtccgctcg cgggagcctg cagatccacg tcaaccaggg tccgaaccac gacccgatcg
       61 cttcggttcc tacgcgcatc acgatggtcg cgggaactgt gtggcggaag acgctcgtgc
      121 ttcgggatct cgacgggagg agcctgtcgt tccagaaggc atcgggtccc gacttcctca
      181 ccgcgggcgt cgtcggacag ggcgagggtg gagcggtcgg cttccttcgt ctaggcccct
      241 cctcctgcga cgcgggcgag tatgaggcgt cgcttaccgt cggcgccggc gccacggccg
      301 cgacccgctc ggtccacgtc tcggtgcgcc cggcgatggc tgcgccgccg agtgatctga
      361 ttgaactgcc gacacggttg tatccctccg gccttgccgg cggcgacctg aacggggacg
      421 ggctcatgga cctggtcgtg gtgaacgagc tccattccct ctccgtcttc gtgtcgctcg
      481 ggggcggagc gttcgcaacg gtgcggtcct acccgctggg cgcctacccg atcatcccca
      541 cctccacaac cctggccgac ttcgatgagg acggacggct ggacgcggcg gtcggcgaga
      601 gcggcgccat caaggtgttc cgcggacgtg gcgacggctc cttcgaaccg ccctccgcgc
      661 ttcccgcaaa ggtgctgccg atggacgtga ttgcggcgga tctcaacgat gacggccacc
      721 tggacgtggc ggccgccgac ggcggcacgt cggccgtttc gctcttcctg ggccgcggcg
      781 acggcacgtt cgcgccgcga aaggacattc ccgtgccggg aacgccgtac ggcctgacct
      841 cgggggattg gaaccgggac ggcagggtcg acctggcggt cgcgggcgct ttcttcagca
      901 aggtttcgat cctgctcggg cacggcgacg gcacgttcca cgaccccacg tcgctgccgg
      961 cgggccgttc cccgttcagc gtcgcgacgg gcgattggga caacgatggg aagctcgacc
     1021 tcgccgtcgc ggacttcaac ggatccctgt tcatcgatcg aggtgacggc gccgggggct
     1081 tcgcgcagac cgacgagttc gacggattca cggccccgca gacggtcacc gcaggcgatc
     1141 tcgatgggga cggtatcgac gacctcgttc tggcggacgg cgggggcagc aaggcgcagg
     1201 tcttccgggg cgacggggcc ggagggttcg tcgcctccga gacgatcgcg cttcccggcg
     1261 gcgcccatca ctgcgcgatc ggggacgtgg acggcgacgc ggtgcccgat ctggcgatga
     1321 ccgaccagag gtacggcgcg gcgctggtcc ggcgcttcgc gtcgcctcag aatgccgccg
     1381 gattcgcgcg cgcgttcgtc accggcgacc atcgcccggt catcatcgcc tcccggacgc
     1441 cggcgttcac cgtgcaggtg gaaccggtcg cggaatcgtt cgcgatccag gacgtcgatc
     1501 tcgagtcgct ccgcctcgcc tccgagggca ccggctcggt cgcctcgatc ggagggatcc
     1561 tggccaaggg cgcctccgcg ggcgaccggg accacaacgg catcctcgag ctctcgctgt
     1621 ggtttggcgg agaagatctg gccaggctct tcgatcgcgt caccgggcgg cgcgacgtcc
     1681 ccgtgcaatt cgaaggacgt ctcctgagcg ggcggctctt ctgcacgcgg ctcgtcctga
     1741 ccgtgcaggg tcccggctcc ccgatgaccg cctcggtggc gccgaatccc ttgaacccga
     1801 gcggcgtgct ccgattcgtg accacgatcc gggggccgct ccacgtcgcc ctcttcgaca
     1861 cgcaggggcg gcgcgtgcgc acgctcgtcg agacgaagga cgcgccggca ggcgcgcagc
     1921 ggatcgcgat cgacgggcgc accgactcgg gcagcgcgct cgcttcggga atctatttct
     1981 tccgcatcga ggccgcggag ggatcgaccc gcggacgggt gacgatcctc aagtagagtc
     2041 gcgcgcgccg cgcgaaccgg gccgaagccc cgtggagtcc cgcggggttt cggcccatac
     2101 tattcggcca tgacgcccga cgacccggcc accccgcctg cggccgcgcc cccagcgcgg
     2161 accctgttcc tgctctcgcc cgccaactgc ggcggcaggc gcgcacagat cctccggagc
     2221 gagcgcgcgg cgttccccct ggccgtccag ctccgggagg cgggcgcccc gcttggagag
     2281 gtcttctcgt tcctgagcgg gctctatttc cggggaaagc tggcgtacgg gatggcgttc
     2341 gggagcgccg cgacccgccc gggcgcgctg gtcatcacgc cgggacgggg gctggtcccg
     2401 gccgacacga tcgtccgcat ggacgacctc gaggagatcg cgagcatcgg cgtctcccag
     2461 gacgagccgc gcttccgcga gccgctcgag cgcgacgccg cggcgctcgc cgccacgctg
     2521 gacgccggcg accgcgtcgt cctgctcggc agcatcgcga ccggaaagta cgtggacgtg
     2581 atcgagcggc tcctggacgg caggctcttc ttccccgccg cgttcgtggg acgcggcgac
     2641 atgagccgcg gcgggctcat gctccgctgc gtgctccgcg gagaagagct ggagtacgtc
     2701 cccatcgcgg gcgcgatccg ccgcggcccg cggccgcccc ggctggagcc gatccggtga
     2761 gcccgccgcg cgcgccggcc gagcgccccg ccaagggccc ggtcaagcgc cccgccaagc
     2821 ccaaggccat ggcgattccc gcggacgtcc gggacgtgga gctggacttc ggcggcggga
     2881 aggtcgtccg gctgaccaac ctcgacaagc ccttctggcc cgagctgggc atcgcaaagc
     2941 gcgacctgct ccagtactac gccggcatcg ccccggtcct gttgccgcat cttcgcgacc
     3001 gcgccatggt catgaagcgc tatcccaacg gcgcgcacgg ccagttcttc ttcatgaagc
     3061 gcgccccctc gccgcgtccc gagtggatcg agacctgcag catcccccac gattcgggaa
     3121 acgtcatcga cttccccatg atccaggatc tcccctcgct cctctgggtg atcaacctcg
     3181 gctgcatcga cctgaatccc tggtacgcgc gctgcgacga caccgaccgc cccgactacc
     3241 tccacttcga tctcgacccg gtgcccggcg cttcgttcga caaggtgcgg gagacggcgc
     3301 tcctggtgcg gcgcggcctc gaggggctgg gcatgacgtg ccatgccaag acgacgggat
     3361 ccaaagggat ccacgtctac atcccgatcc gccgcgggcc gctgcagaag gaggtctggg
     3421 ggtttgcgaa gagcttcgcg atcgcgctgg ccgcccaggc tccggcgctg gtcaccgcgg
     3481 aataccgcgt cgccaagcgg cccgcgggcc gcgtgctggt ggactacaac cagaacgcgt
     3541 ggggccggac gctggcctcc atctactccg tgcggccccg gccgcgcgcc ccggtctcga
     3601 cgccggtctc gtggaaggag atcgagcgcg gcatcgagat cgaggacttc cgaatcgaca
     3661 acgtgccgca gcgcgtggcc aagctcggcg acctgtggaa gccgctcctc ggcgcgaagg
     3721 gccgggtagc cctggagaag ttcctatgac ccttccgatc cgacgcccct tccctccgat
     3781 ggaagcgcgt tccgtggacg tcatccccgt cggaccggaa tgggaatacg agcccaagtg
     3841 ggacggcttc cgctgcctgg cgtttcgcga cggcgacctg gtggcgctgc agtcgaaggc
     3901 gggcgagccg ctcgagcgct acttcccgga gatggtggag gcgcttcgcg aggtcaaggc
     3961 accccgcttc gtggtcgacg gcgagatcgt ggttccgagc ggcacgtccc tctcgttcga
     4021 cgacctcctg atgcgcatcc accccgcgga gagccgggtg cggaagctcg cggccgagac
     4081 cccttcgatg ctcatcgtgt tcgatctctt ggtggacgag aagggcgctt cgctcgtggc
     4141 gctgcccttg accgagcgcc ggccgcggct ggaggccttc gcggcgcgct tcttcgagaa
     4201 tcaccgctcg atccggctct cccccgcctc ggccgacccg cgcgacgcga tgcgctggta
     4261 cgagaagacg cggggcgccc tggacggcgt gatcgccaag cgccgcgatc tcccctaccg
     4321 atcgggcgac cgcagcggga tggtcaagat caagcagcgg cgcaccgccg actgcgtcgt
     4381 gggcggattc cgttacgcgt cgaaggggaa aagcgaagtc ggctccctcc tgctcggcct
     4441 gtacgacgac cagggcctcc tgcatcacgt gggattcacg tcgagcttct cggctcccga
     4501 gaggaaggcg ctcgtgccga agctccaggc gctcgtgaag ccccccggtt tcaccggcaa
     4561 cgcgcccggc gggccgagcc gctggagcac ggagcgctcc ggcgagtggc agccgctcca
     4621 gccgaagctc gtggtcgagg tggagtacga ccacgtgagc ggcagccgct tccggcacgg
     4681 gaccaagttc ctgcgctggc gcccggacaa ggcgccgaag cagtgcacct tcgaacagat
     4741 cgagcggccg gaagggtcgc tgttgcagct gttgggggcc ggggtgcgcc ccaaggccgc
     4801 gcggagcgct agaccttcgt cgcgcgggtc atgaggcagt cttgcagcac gacgtcttcc
     4861 gctgggtctc cttgaaatga gccaaagcgt ccatcggagc ctccggatcc agaatcggtt
     4921 tggggtcgcc ggaaatgacc acctccgatg cccggcgccg tcaaccggac gggtccaacg
     4981 ctccggccgg gtggcctgtc taagggcatg acgcgagcac gcccaagacc cctgaatcga
     5041 ggagacgcca tgcaccccat tcgcgtagcg gcagtggccg catcggtcgt tctgattctc
     5101 gcggccccct cgctttcccg agcacacggc gattcccacg ccaatgacca cggctttgcc
     5161 tacggcaatc atggggacga cttcacgtgg gccatcgtgg acggcgggaa cacgagcatg
     5221 tccgggcagg tggaccatcc cacgctggag gaactcaaag agcgcttcgg agaacgcttc
     5281 ctctacatcg aggacgacga ggacgggtac gtgatcaccg accgcgccat ggtcgagcgc
     5341 gcccgccgcg ccacggagcg gatcggccgg tatgggcgcg agatcggcga gatcgcgcgg
     5401 acccaggcga agctctcgct cagcgaggcg cggcccaaga tccgccgggc ggagctcttg
     5461 cgcgagcagg cggagctccg gcgggagatc gaccgggccg agcggcaagg cgagccgacc
     5521 gaccgcctcg agcgaaggct ggaccgcgtg acgacccggc tcgaggtgga agaccaggtg
     5581 tacggtggat tcgcgctctc ctccgacgag aagcgagacc tgacgcgccg gcgcgacgag
     5641 gcgaaagcgc gcctgcacga ggcggtggag caggtcaatc gggagatgcg ggacatcctg
     5701 cgcgacgcca aggaccggcg catcgcggag ccggtggact gaatcgcgac gggtccggcg
     5761 cgacgccacg caggccggca cgcgctggtg tggaacggcg ggcgggggcc ctgtacgatt
     5821 ccggcgtgca tcccccgacc gtcgtcctcg aaacgccgcg cctcacgctt cgccgcttca
     5881 ccctcgagga cgcgccgttc gtcttcgaga tggtgaacga ccccgactgg atccgctaca
     5941 tcggcgaccg gggcgtacgg accctggacg acgcgcggac ctacatccgc gaccgcacgc
     6001 tcgcccagta cgaccgcgtc gggttcggca tgtacgtcgt cgcgctgcgg cacgggggcg
     6061 aggtcgtggg atcctgcggg ctcatccggc gggactcgct ggacgacgtg gatatcggat
     6121 tcgcctttct ggcccgccat cgcggacagg gctacgcgac ggaggcggcc gcggcggtgc
     6181 tggattacgg ggtgcgctcc gtgggcctca cgcggatcgt cgcgatcgtc gcgtccgaca
     6241 atcaccgctc catccggatc ctcgagcgga tcggaatgca cttcgagcgg atgatccggc
     6301 tccccggcga ggacgaggag atcccgctct acgcctacga ggcgccgggc gcggccgcct
     6361 gaagaggtca gtactccacg aacgactcgt cgggatagca gtacagccac cgctctcccg
     6421 gctccgccga cgccaccacc gggtggtgga ccgccttcgc gtgcttgctc gcgtgcttgt
     6481 tcggcgagga gtcgcagcag agtgtggtcc cgcactgctg gcaggtgcgc aggtgcaccc
     6541 accgcgctcc gatcggcacg cactcgtcac actggcgcaa cttcggatgc ttcacgtcat
     6601 ggatctgttt cagatgggcg cacgcctcgt ccatggcact acctccgttc cagctcgtcg
     6661 cgatcccagc cggcgagcct ggcgccggcc tcgtaggtgc tgttcaggaa cgccgtgagc
     6721 gccgcgaccg ggtccgccga ctggcgaacc gcttcgaacg gcaggatgaa ctcgccgaac
     6781 tcccggttgt aggacgccgc ggcgggttcc acggccgccg cttcgaaacc cgccggcgcc
     6841 ggcgccgcgt acgcgtagaa cgccgcgtcc ggcaccgctc cgctccccgg ccagaagccg
     6901 tggctgat
//
LOCUS       DASSLZ010000350        24551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1874063, whole genome shotgun sequence.
ACCESSION   DASSLZ010000350 DASSLZ010000000
VERSION     DASSLZ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 24551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24551
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874063"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..679
                     /locus_tag="VFM00_13215"
     CDS             41..679
                     /locus_tag="VFM00_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499252.1"
                     /translation="MGSRAARMLVAVMAATAAWSAFAWSAAAPARAASLPGAHRSPLE
                     RSPGYYPPADPESASVRLGRRKAKPVRLLLSGGSRSIDDLAQRLLAGLEAKDEKALHA
                     CRLTRSEFALICWPEFPESRPITRITADDAWELSDPTSVAGASRALSMVGGRPLSFVS
                     VHATSREAYRNFVLHRGVVIEARDESGSMVSLRFAPSVVERRGRFKVLMFKD"
     gene            1002..2150
                     /locus_tag="VFM00_13220"
     CDS             1002..2150
                     /locus_tag="VFM00_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499253.1"
                     /translation="MKRIGLWTAAALGCALAAPAPAQTPSNPPPPVVETAPDFPRGKI
                     SGLVFGDVYYNVTGDPNHVYTNGADGLPANLDGSSPSASSPAAQPKVIGKDLNGLQVR
                     RIYFQLDNDLSIRVATRFRLEADSKELTSGGKIGVFVKAAYVQVKDLLPRGNLLVGMT
                     NTPTFENSEEFWGYRSVEKTIVDFRGLASSSDLGLNLKGFLDGGHRIGYSGMIGDGTG
                     QKPENNRYKRMYLAVPLRLIEDLRIEPYVDYEDFAGNADRALYKLFLGYELKRAAIGA
                     EVVDQVVHVDGARNREPFGISVFGRAHASDQLSAFARYDRWQPDTRAANRIDSDLYIA
                     GLDWEPIKDLHFMPNVEATGYRARGTAAAPPHHDLQARITFYYRFSKP"
     gene            2170..3237
                     /gene="pstS"
                     /locus_tag="VFM00_13225"
     CDS             2170..3237
                     /gene="pstS"
                     /locus_tag="VFM00_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7499254.1"
                     /translation="MKIRITKALAVALLLLAALAPAAAPRAARAETNLTGAGATFPYP
                     LYSKWFDMYHEKTGVQINYQSIGSGAGIQQVKAGTVDFGASDAALSNQRLKEMPRTVL
                     HFPTAAGAVVLAYNLPGVKEPLNLTPEAIAGIYLGTITMWNDKAIAAANPGVALPTAP
                     ILAVHRSDGSGTTNIFTTYLSAVNRHWKELVGANTSVSWPVGIGGKGNEGVSGLVRQT
                     SGGIGYIELAYAKQNQLAYARVRNSSGKFIEPSLASTTAAAQGAAALLAKDVRTPIVN
                     SPAPDAYPISGMTFLLVYRDQKDAAKAKTMADFIQWANGEGQQVNETLDYARLPEQVV
                     RVNQATLMMLRVGGRPVLAAK"
     gene            3274..4338
                     /gene="pstS"
                     /locus_tag="VFM00_13230"
     CDS             3274..4338
                     /gene="pstS"
                     /locus_tag="VFM00_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7499255.1"
                     /translation="MNRYVRFLLTAALLVGVAAGCSKGGGAGAGTTLTGAGATFPYPL
                     YSKWFDAYKQKTGVQINYQSIGSGGGIQQLKAGTVDFGASDAPLADDKLREMPHTVIH
                     FPTVGGAVVLAYNLPGLKQPLQLTTGALIAIYTGRVTKWNDARIAAANVGVTLPNLAI
                     LPVHRSDGSGTTNIFTTYLSAISQTWRDSVGANTSVSWPNGIGGKGNDGVAGQVRQTP
                     GSIGYVELAYAKQNDLAVVHLQNAAGAYVEPTLASTTAAIQASAAALAQDVRTPVVNA
                     KAPDAYPIAGLTFLLVDADQQDAAKAKAIAQFIQWAITDGQAMAEPLFYAKLPAEVVQ
                     VNQAALTKITAGGKPVLASR"
     gene            4335..5324
                     /gene="pstC"
                     /locus_tag="VFM00_13235"
     CDS             4335..5324
                     /gene="pstC"
                     /locus_tag="VFM00_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7499256.1"
                     /translation="MNRTATRSSSGTGPEIARPLARRGLTLDPIYKALLMAAAATVFL
                     VVALIVFEVARGSWLSIKTIGWSFLVQSQWDPVADHYGALPYVYGTLVSSLLALVLAV
                     PLGIGSAVFLAELASHRVGSIVSFVMELLAAIPSIVYGIWGFFVLAPWLRSTLEPWLI
                     ANWGAIPLFRGTPFGIGMLNAGIVLAIMILPTIVSISREVLLATPRTLREASLALGAT
                     RAEAIGVTLDAAKPGILGAVILALGRALGETMAVTMVIGNTNRISASLLSPAATMSSV
                     IANEFAEATGDLYLSALIQIALVLFAVTIVVNALARALVYLTTGGRRDAGAAA"
     gene            5321..6211
                     /gene="pstA"
                     /locus_tag="VFM00_13240"
     CDS             5321..6211
                     /gene="pstA"
                     /locus_tag="VFM00_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET7499257.1"
                     /translation="MSAAVRKSAEGWSDPAPARVRLWRGFANRVVLGACYAAAALVVL
                     PLVLIVWHLVAKGLPALNLSFFVHMPKPVGEPGGGMANAIVGTLVVVGMGALIALPLG
                     IGSGVYLAEYGRGRFAMMVRYTTDVLSGVPSIVVGVVAYGLVVVPMGRFSAIAGSVAL
                     AILMLPPIIRATEEMVRLVPQSYRQGALALGAPRWKVIQQVVLPAARAGIVTASMLAI
                     ARAAGETAPLLFTALGNRFWSLRLDQPIATLPVFIFDYARAPYADWNRQAWTGALVLV
                     LLVAVVSGVLRLTVRRIRVR"
     gene            complement(6237..6920)
                     /gene="phoU"
                     /locus_tag="VFM00_13245"
     CDS             complement(6237..6920)
                     /gene="phoU"
                     /locus_tag="VFM00_13245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET7499258.1"
                     /translation="MERHFHHDLHQLQDRLSDMAGRAETALSKAMEALKTRDTVLARD
                     VMTEDLEIDRIELEVERRCLDFLGLQQPVARDLRFLVASIRVSNFLERIGDHAVNIAQ
                     SAIKLSGMPPWKGPEDLATMGDRTIAMLRDAVSAWLNHDAPLAKRVCENDAEVDTLKT
                     QIFARFSSQMVHEPEIVPRALEMVLASRNLERVADLATNIAEEAIFVTEARVIKHHAD
                     EIRDATLGS"
     gene            complement(6985..7878)
                     /gene="pstB"
                     /locus_tag="VFM00_13250"
     CDS             complement(6985..7878)
                     /gene="pstB"
                     /locus_tag="VFM00_13250"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HET7499259.1"
                     /translation="MDPTATLPERRTRPNGPRAPLVPLVPEEPDSDVSPAPAPIERED
                     ASAAPILQTKRLSLFYGSHAALKGVDLEIGQNRITAIIGPSGCGKSTLLRCFNRMNDL
                     FPPVRYEGNVLFHGSDILSPGTDLVALRRRVGMVFQRANPFPMSIFQNVAYGPKLQGT
                     RDRRRLEEIVESALRRSALWDEVKDRLGRSALGLSGGQQQRLCIARALAVEPEVLLLD
                     EPASALDPTATAKIEELTLEIKNGVTVVMVTHNMQQAARVSDRTVFMLNGERIEEGPT
                     RTLFTSPREKLTEDYITGRFG"
     gene            complement(7937..9340)
                     /locus_tag="VFM00_13255"
     CDS             complement(7937..9340)
                     /locus_tag="VFM00_13255"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7499260.1"
                     /translation="MRSLTGRVYFLLLLALLLAACAVGVLLNALKPVGAGGAIGIVAS
                     VLAVALGAGWLPFRSLNRRFAHIADAASWMAAGDVSRRAPVDAHDELARLARSLNDIA
                     DSFESKLKELQQGRNASRAILGNVPLGLALVAPDLSIRHANARFWEMLELDHPPRDPR
                     LSSTRQPVLEQMALHALEHRKPLVRDLTLYLADRRDYEVRASPIGAYAGAEPETILVS
                     LEDLGPVKAMAAVRREFVANASHELKTPLTSIRGYAETLLSGGLEDEGNRTRFVETIR
                     DQASRLEALVEDLLDLADLERPDAPLDLKDWDLGEIVRDLASTFEDVAARRGLKLSLE
                     SRAGIRARVDRKRLELALRNLMDNAVKYTERGTVEVAVEDQGDSVRIRVTDTGRGIEA
                     EHLARVFERFYRVDRGRSRALGGTGLGLSIVKHAVQLHGGTVGVESAAGQGSTFWVEV
                     PREGPGPEPRGPGPSGA"
     gene            complement(9362..10060)
                     /locus_tag="VFM00_13260"
     CDS             complement(9362..10060)
                     /locus_tag="VFM00_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7499261.1"
                     /translation="MPGTARSTILVVDDEAEICDLVSYNLGREGYRVIAENDGAAGLD
                     RIFKSPPDLVVLDLLLPKLSGLEVLDAIRGEARTKGLPVLVLTARGTEMDKLVGFERG
                     ADDYLTKPFSPKELVARVSAILRRTRGEERQASTVIGPVRFDPDRHQVFVRERELRLT
                     PTEYRLLEFLLERPGRVFSREQLLDKVWGLGYFGETRTVDVHVRRLRAKLGKDAGLIR
                     TVTGVGYAAEVPKG"
     gene            10302..11918
                     /gene="secD"
                     /locus_tag="VFM00_13265"
     CDS             10302..11918
                     /gene="secD"
                     /locus_tag="VFM00_13265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET7499262.1"
                     /translation="MTQSDRNKLFLTLIGLAAALYTLWPTFQFYALSPAKRAEVLQAR
                     PATATNDADRDRLTKMAKLRDRSIKLGLDLQGGLYLLLEVDKSKLGPAEAKGAVDQAM
                     QIIRNRIDQFGVAEPSIQKQGDNRILVQLPGLLDQERAKELIGQTALLDFKIVKNDDE
                     VRALFDRIDQYFTRKMAAATGAKPDSTSHPWTSRFLTVAQAGESFALPENEAAIDSMK
                     NQLLADSSFTMDATLAWDAHEGAHESRTGRSLYVLSKEPLMQGKEVASAQMRMDLDQE
                     RPGAPGVSFTLTGRGGAVFADVTGANVGRRLAIVLDNRVQSAPNIHERIPRGQGSITG
                     SFTEEEAQNLSIVLRSGALPAPVNIVEERAVGPSLGQDSIQRGIMAGAIGTVLVVVFM
                     VVYYRMSGMVAVAALLLNIIGLLACMAAFHATLTLPGIAGIVLTIGMAVDANVLIFER
                     IREELRHKKTVLTAIELGYSRAYRTIVDANVTTLLSALALMWFGTGPIRGFAITLSIG
                     LIINMITAVGVSKMIFDAWSLRRKVSTISI"
     gene            11964..12842
                     /gene="secF"
                     /locus_tag="VFM00_13270"
     CDS             11964..12842
                     /gene="secF"
                     /locus_tag="VFM00_13270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET7499263.1"
                     /translation="MRRRHIAYVVSGLSILASIASLVMHGGPRYGVDFTGGTLIEVSI
                     QPPPSVDDVRRGIDQAGFKGAEIQRLERPDLLLIRMQVQEGRPSPAPAVRQALLAAHP
                     GSTVDVRRVENVGPRVGGELRAAAVKAVFLALGMILVYVAIRYDWRYSMGAVVALFHD
                     VFITLGAVSIFNKEVTLTVVAALLTIAGFSINDTIVVFDRIRERRQALRREPAEKVMN
                     IAINETLSRTIITSFTVVLTVLALFFLGGEVLHDFSFAMLVGVIYGTYSSVYVASALA
                     LETQLALERRTPARKR"
     gene            12853..13386
                     /locus_tag="VFM00_13275"
     CDS             12853..13386
                     /locus_tag="VFM00_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499264.1"
                     /translation="MRPAALPARTAAMLLLAGSAAAGPSLCRAEAPPPPPPPVSTPIL
                     APDSRAEAHPAGPTATPLQLFAAAEEAWGSGDAEGLASLVDTTQVRIGLKPGSTPTAA
                     MTRSAAAFLFQDQLRLVTTQSFQVTRINVGNTSATATARWWGNWGGREGNRKLTVTLS
                     AVPLAGRWYLREVRVKG"
     gene            13436..14326
                     /locus_tag="VFM00_13280"
     CDS             13436..14326
                     /locus_tag="VFM00_13280"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7499265.1"
                     /translation="MNDADVLYTSDLHGSRSHYEAAFRMAADLGVRAVVLGGDLAPMG
                     DPAAQREFFQTFLIPLLRERLAAPGAPPVFYIFGNGDWRANEAALEEARLPGLHYVHG
                     RAAPFLDGTWIAGQNCVPPTPIRLKDWERWEVVPGASGRPDGHRSAPDGTLHDFTFVG
                     REREETLEAEMDGLETAVRGATGSAEAAAVAPQALVCVFHGPPHGTALDQIAGGTHVG
                     SGAARRFLQRMRPALSLHGHIHESPSVSGRFADRVGGTVCVNPGQTPAAFHAVSFRMA
                     DIAGTLRHTLHGPANLAEPR"
     gene            14383..15183
                     /locus_tag="VFM00_13285"
     CDS             14383..15183
                     /locus_tag="VFM00_13285"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET7499266.1"
                     /translation="MAEVDDRADPTLDEALLPQDLTLSQWFRVVRWAGRGAVAGAIAA
                     ILAAALAGPAAGQGPPASAGADLTGGFSVARLKYTGGGDWYSDETSLKNLLSALKARG
                     DIRVSQQKEAVVTATDPDLWNYPLLFVTGHGNVKLNDQEVASLRRYLDQGGMLWGDDN
                     FGMDKSFREAIQRVYPESPLVELPFNHEIFRRPNPFPSGAPKIHEHDGGPARVFAIMR
                     GGRMVVLYTFDTDIGNGIEDAGVHDDPQAKRDAAMRFAINIATYAVSH"
     gene            15211..18549
                     /locus_tag="VFM00_13290"
     CDS             15211..18549
                     /locus_tag="VFM00_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499267.1"
                     /translation="MTPSGDSLHATLSRARRFLVWRAVEQAGLALVVAAILVALIALG
                     VALATPLHRDEFVLIRLGLLGSGALLLLFAAARVVLAPARLEDAALATGRLLGERDDE
                     LLGALELARAGADESRDSGALRDAAVGAAAARAAGAPIARLRRWDARGRWLLAAGGTL
                     VLLGATAVAGGARTPAVLRHIADPRTAPIAPIAIRVEPGTRDIEGGESVVIRAYVRGS
                     GQRPKLEVEDRGDWNETSLDEAEDATGVKSGERAWSLLLRNLKDDVRYKVRASGQESP
                     VYALTVRDLPRATGYRVRYDYPPYTGLKSEESHAVTGDLAAPRGTRARLVVTLSRSVR
                     RGALLFEGGTSVPAEPGERLAAFTVPIRGDDRYAARLEDARGRRVDLGPFEVRAIPDR
                     PPTVSVLSPGSVEDVGRDMTATIIAGATDDYGVRKILIRYKVRDGAEKTEVLHEEKGT
                     ARELAVRYTWALQSYSLLPGEEVEYRIGAMDGNAVDGPQTTWSDARLLRFPSASEILA
                     SMSGERDETIHSLEDAWQDAKDLSQKAEELSRDLGRTREMTWEKQQEAQKTLEGQQQL
                     QQKIDQVADKLSQDAEKLSQSHALNSELAQKIQELHEILSQIKDQSLLRSIQRLQDAM
                     KNLSPEQMQAALQNLKVNQEQVMKSLERTIEMLKQIRTEDKMEAASERAAEMERRQIA
                     LNDSLSRAKKSEETKSLAKSQRENEKLSAEERAALDSLAADLKAMDPEASKESEQLAD
                     DLGEQGAKPDFQHASESMEQGDRENSRQAAERLKQRLTQLRQGVDKMREKFQNKRKNE
                     LAKKMEDAAQDLLDIGRLQQKMLNDQESSLSERAETQKGLEDATTAVTKRIADIGKQT
                     LHITPDIGQALGRALTNQDNSVGRYSEQDLGGGLMAGKEAAIALNQAAAGLLRSKQSM
                     QSSSSSTGFQEAMDRLQSLAGEQEQLNSQSMGMMPGEGQQEGSQANRLSEGEGQALSR
                     MAAEQEAIRRGLEETMQKLGKGGGTLGNMGDVADEMKKVEQDLRGGRLNQETTERQQR
                     ILSRLLDAPRSVEKRDYSRKRQSRPGVDVVRSSPGALSPELLRARPSLAALLARAGRD
                     PVAPRYRALVDEYLQSVLGGGKPK"
     gene            18546..19355
                     /locus_tag="VFM00_13295"
     CDS             18546..19355
                     /locus_tag="VFM00_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499268.1"
                     /translation="MTRHSIGTRRAGRAAIALASAALLVTAASGSAGAKPRPEPPAKA
                     EPRVAPRVPQPLPLLNRDDRLPEGYQDLVRRLMARGPGDTLGDGVFTLAEGSFHAGAF
                     DEAGGRYADFAQRFPRNLRVNLALERLLLMKDGRDFGDEPLRIYARADLMRAEGHADS
                     AETALTAGLARYPGTKLRYHFRYALAEIARDKGNHAAAIEQALAVADTSAGSRLAPAA
                     LKLAGDETLAMGGPPERASGYYQSLLERFPDSPLAPSVRERLLLLRKKMQL"
     gene            19448..20625
                     /locus_tag="VFM00_13300"
                     /pseudo
     CDS             19448..20625
                     /locus_tag="VFM00_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
     gene            20663..21637
                     /locus_tag="VFM00_13305"
     CDS             20663..21637
                     /locus_tag="VFM00_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7499269.1"
                     /translation="MYDPSREYTTSVLIAGVTGAIGRAVARDLIVNGYRVYGLIRDAE
                     SRDRLPYGVVGVVGDVRAPEKWETAIERTQVVVNCAFPRDLSTGALTYEDAEREADEL
                     AAILDRLCDLVRRRKRRLIHTFGALLYEPDANGWVRETSAIASGRGYGVRHRRTYPVF
                     EKHRKKGLKAMSVNPAFVYGRGGWFEYGLVQPLVRGQAVLIGDGSQTMHYVAASDAAS
                     GYRLAIEHGLDGEDYLLGDDRPTTLGEFTRLVARELGVAEPRSVPEEELVAQLGAWNV
                     DAYTFCPKVDSTKARERLGWTPRYPTIEEGVPAAIRDYKRAQLQAASG"
     gene            21643..23250
                     /locus_tag="VFM00_13310"
     CDS             21643..23250
                     /locus_tag="VFM00_13310"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET7499270.1"
                     /translation="MRLRPERRRFAVPLRAALVLSLLASCAPAPADSWWRHVRWLADD
                     ARQGRETGSPGYLAAAQYVAHEFETAGARPAGTSGYFQPVAFIARRLVEPECLVTLAR
                     ADRTDTLVLGEDALLSSSIDAADSVDAPAVFAGHGLELPEQGLDDLAGLDLRGAVVFY
                     LRGAPREVPSTVAAHAQSTGERWARLRERGAIGMVTISNPKARSIPWERARLSRLEEG
                     FALADSTLDEKGGMRFSMYFNPDRAERLFQGTGHTFAEMLQLADQNRPLPRFTLPFRI
                     RARVRMTARNAESPNVVGVIPGSDPALRDESVVLTAHLDHLGIGAPVDGDSIYNGAMD
                     NASGVATLIEIAHALRGGPPPRRSVVLLALTAEEEGLLGSSWFAAHPPAAVGRMVASL
                     NLDMFLPIAPLRSVIAYGERESDLGDWFRAVADSAGLKVEPDPEPAQNYFIRSDQYSL
                     VRAGVPSLFADIGAGGDSTLVRRFQLWRKNRYHAPSDDTRQPIDFRSADDFNRLLLRF
                     TRDVANRPERARWKADSFFRRYQRVPS"
     gene            complement(23247..23732)
                     /locus_tag="VFM00_13315"
     CDS             complement(23247..23732)
                     /locus_tag="VFM00_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7499271.1"
                     /translation="MLRGFSKGLTMLELAVAMTVVGILAAIAIPNYMNFMRDVRAAQA
                     VADIQAVRAAVYLYFGDHQQWPPEEQAGVVPVELTPYLPRGMVFFKSAYKLDYDNWMV
                     NGGGGVTSRFPGTGVLVGVSVISGDREMIKAAKSLLGGQNTVNLSPLRTTLVVASETG
                     F"
     gene            complement(24018..>24551)
                     /locus_tag="VFM00_13320"
     CDS             complement(24018..>24551)
                     /locus_tag="VFM00_13320"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HET7499272.1"
                     /translation="RYDDTQYLDQRPVSIFGGQGIGGISYIFGDIHQQTASVTLRSSV
                     LFTRNQSLEIYAEPFLTVGKYSRARELARADSYDFVNYAGHNVDASNNPTTPYDFRDN
                     DFSYSSMNVNVVYRWQYRPGSTFFLVWKQSRERFEDRSMFSIDPNRFENGLGTRTLFR
                     NEPENTLLAKITYWLPI"
BASE COUNT         3789 a         8568 c         8481 g         3713 t
ORIGIN      
        1 tatcgcaccg gccgcaccct ctccctggcg atgagccttc gtgggtagcc gcgcggctcg
       61 aatgctcgtc gccgtcatgg ccgcgaccgc ggcatggagc gctttcgcgt ggagcgcggc
      121 ggcgccggcg cgcgcggctt cgctcccggg agcgcatcgc tcgccgctgg agcgctcgcc
      181 cgggtactac ccgccggccg atcccgaatc cgcctcggtg cgcctcggcc ggcggaaggc
      241 gaagccggtg cgcctgctcc tgtccggagg atcgcgctcc atcgacgatc tggcccagcg
      301 gctcctcgcc gggctcgagg ccaaggacga gaaggcgctc cacgcctgcc ggctcacgcg
      361 gagcgaattc gcgctgatct gctggcccga gtttccggag agccgtccca tcacgcggat
      421 caccgccgac gacgcgtggg agctgtccga tcccacgagc gtggccgggg cttcccgcgc
      481 gctctcgatg gtcggagggc ggccgctctc cttcgtcagc gtgcacgcga cgtcgcggga
      541 ggcgtatcgg aatttcgtgc tgcaccgcgg ggtcgtgatc gaggcgcgcg acgagtccgg
      601 ctcgatggtg tcgcttcgat tcgccccgtc ggtggtggag cgtaggggcc ggttcaaggt
      661 gctgatgttc aaggactgac gggccgcgct ggtcgtgtcg ttcgggactt cccccgagcc
      721 caaactgaag cgaacgcaag attgcgtccc ggggatcggg cagaggtcgg acgagcgagc
      781 cggcgcggcg cggggccggg cgggccatcc cgaagcgccg caggccgttg ccgcgcgttc
      841 cctccgctcc cgcctgccat gttacgaccg taacggataa ggcacggttt cgtcataact
      901 cggttacaag cgcttcctag tctgaactcg acgaagatgg agtcccgcga gcggggacgt
      961 gccccggatc gcgaacctgg acccgcgtca taggagcgct catgaagcga attggtttgt
     1021 ggaccgcggc agcgctggga tgcgccctcg cggcgccggc gccggctcag acgccttcga
     1081 accctccccc gcccgtcgtc gagaccgctc ccgacttccc acggggaaag atcagcgggc
     1141 tcgtgttcgg cgacgtgtac tacaacgtga ccggagaccc gaatcacgtg tacacgaatg
     1201 gcgcggacgg cctgccggcg aacctcgacg gctcgtcgcc ttcggcgagc agtccggcgg
     1261 cacagccgaa ggtgatcggg aaggacctga acgggcttca ggttcgccgg atctacttcc
     1321 agttggataa cgacctctcc attcgcgtcg cgacccggtt ccgcctggaa gccgacagca
     1381 aggagctcac gtcgggcggc aagatcggcg tcttcgtgaa ggccgcctac gtccaggtga
     1441 aggatctgct gccgcggggg aacctgctcg tcggaatgac gaacacgccc acgttcgaga
     1501 actccgagga gttctggggc taccgctcgg tcgagaagac gatcgtggat ttccgggggc
     1561 tcgcctccag ctcggacctc ggcctcaatc tcaagggctt cctcgacggg gggcaccgca
     1621 tcggctactc ggggatgatc ggcgacggca cgggccagaa gccggagaac aaccggtaca
     1681 agcggatgta cctggcggtg ccgctgcgct tgatcgaaga cctccggatc gagccgtacg
     1741 tcgattacga ggacttcgcc ggcaacgccg accgggcgct ctacaagctg ttcctgggct
     1801 acgagctcaa gcgcgcggcg atcggcgcgg aagtggtgga ccaggtggtg cacgtcgacg
     1861 gcgcgcgcaa ccgggagccg ttcgggatct cggtgttcgg acgggcccat gcctccgacc
     1921 agctgagcgc cttcgcgcgc tacgaccgct ggcagccgga cacccgcgcg gcgaaccgga
     1981 tcgactccga cctctacatc gcgggtctgg actgggagcc gatcaaggac ctgcacttca
     2041 tgcccaacgt cgaggccacc ggctatcgcg cgcgcggcac ggccgccgcg ccgccgcatc
     2101 acgatcttca ggcgcggatc acgttctact accgcttctc caagccctga ccgacaccgg
     2161 aggaatccga tgaagatccg tatcacgaag gccctggccg tcgcgctcct gctccttgcg
     2221 gcgctcgcgc ccgcggcggc tccgcgcgcc gcgcgcgccg agacgaacct gacgggcgcc
     2281 ggcgcgacgt tcccctatcc gctctactcg aagtggttcg acatgtacca cgagaagacc
     2341 ggcgtgcaga tcaactacca gtcgatcggc tcgggtgccg ggatccagca ggtcaaggcg
     2401 ggtaccgtcg acttcggcgc gagcgacgcc gcgctctcga accagaggct caaggaaatg
     2461 ccgcggacgg tgctccattt cccgacggcg gcgggggcgg tcgtcctcgc gtacaatctc
     2521 cccggcgtga aggagccgtt gaacctgacc ccggaggcga tcgccgggat ctacctcggc
     2581 acgatcacca tgtggaacga caaggcgatc gcggccgcca atccgggtgt cgcccttccc
     2641 accgcgccga tcctggccgt gcaccgttcg gacggcagcg gcaccacgaa catcttcacg
     2701 acctatctct ccgccgtgaa ccgccactgg aaggagctgg tcggcgcgaa cacatcggtc
     2761 tcatggccgg tcggcatcgg cggcaagggg aacgaggggg tgtccggcct ggtgcggcag
     2821 acgtccggcg ggatcggcta catcgagctc gcctacgcga agcagaacca gctggcctac
     2881 gcgcgggtgc ggaactcctc cggcaagttc atcgagccgt cgctggcgtc gacgacggcg
     2941 gccgcgcagg gcgccgccgc tctgctcgcc aaggacgtgc ggaccccgat cgtcaattcc
     3001 ccggcgccgg acgcctatcc gatctccggg atgaccttcc tgctcgtcta ccgcgaccag
     3061 aaggacgcgg ccaaggcgaa gaccatggcc gacttcatcc agtgggcgaa cggcgaagga
     3121 cagcaggtga acgagacgct ggattacgcc cgcctccccg agcaggtcgt ccgcgtgaat
     3181 caggcgacgc tgatgatgct gcgggtcggc ggcagaccgg ttctcgccgc gaagtaattc
     3241 ccggcgggat cccatacatg tgagggagga cccatgaacc gatacgtgcg atttctcctg
     3301 accgccgcgc tcctcgtggg cgtggcggcc ggatgctcga agggaggcgg cgcgggcgct
     3361 ggaacgaccc tcacaggggc gggcgccacg tttccctatc cgctctattc caaatggttc
     3421 gacgcctaca agcagaagac cggcgtgcag atcaactatc agtcgatcgg ctcgggcggc
     3481 gggatccagc agctcaaggc cggcaccgtc gacttcggag cgagcgacgc gccgctcgcg
     3541 gacgacaagc tgcgggagat gccccacacg gtgatccact tcccgaccgt cgggggcgcg
     3601 gtcgtgctcg cgtacaacct ccccggcctc aagcagccgc tgcagctgac caccggcgcg
     3661 ctgatcgcga tctacaccgg cagggtgacg aagtggaacg atgcccggat cgccgcggcc
     3721 aacgtcggcg tgacgctccc aaacctggcc atcctccccg tgcaccgctc cgacgggagc
     3781 gggacgacga acatcttcac gacctatctc tccgcgatca gccagacgtg gagggactcg
     3841 gtgggggcga acacgtcggt gtcctggccg aacggcatcg gcggcaaggg gaatgacggc
     3901 gtggccggcc aggtgcggca gaccccgggc tcgatcggct acgtcgagct ggcctacgcc
     3961 aagcagaacg atctcgcggt ggtgcatctc cagaacgcgg cgggcgccta cgtcgagccg
     4021 acgctggctt cgaccaccgc agccatccaa gcctccgcgg ccgcgctggc ccaggacgtc
     4081 cggacgccgg tcgtgaacgc caaggcgccg gacgcctatc cgatcgcggg gctcaccttc
     4141 cttctcgtcg acgccgacca gcaggacgcg gccaaggcga aagcgatcgc ccagttcatc
     4201 cagtgggcga tcaccgacgg ccaggcgatg gccgagccgc tcttctacgc gaagctcccg
     4261 gcggaagtgg tgcaggtgaa ccaggcggcg ctgaccaaga tcacggccgg cgggaagccg
     4321 gtcctggcgt cgcgttgaac cggaccgcga cgcgctcctc gtcgggcacg gggccggaga
     4381 tcgcccgccc cctggcccgg cgcggcctga cgctcgatcc gatctacaag gcgctcctca
     4441 tggcggcggc ggccacggtg ttcctggtgg tcgcgctgat cgtcttcgag gtcgcgcgcg
     4501 gatcgtggct ctcgatcaag acgatcggct ggagcttcct cgtccagtcc cagtgggacc
     4561 cggtggcgga ccattacggc gctctgccgt acgtgtacgg cacgctcgtc tcttcgctcc
     4621 tggccctcgt cctcgccgtg ccgctcggca ttgggagcgc ggtcttcctc gcggagctcg
     4681 cgtcccaccg ggtcggatcg atcgtctcct tcgtgatgga gctcctcgcg gcgatcccga
     4741 gcatcgtgta cggcatctgg ggcttcttcg tgctcgcgcc ctggctccgc tccacgctgg
     4801 agccgtggct catcgccaac tggggggcga tcccgctctt ccgcggcacg ccgttcggca
     4861 tcggcatgct gaacgcgggg atcgtgctcg cgatcatgat cctgcccacc atcgtctcga
     4921 tctcgcgcga ggtgctcctc gccacgccgc gcacgctgcg cgaggcgtcg ctcgccctgg
     4981 gcgccacgcg cgcggaggcg atcggggtga ccctggacgc cgccaagccg gggatcctgg
     5041 gcgcggtgat cctggccctc gggcgcgcgc tgggcgagac gatggcggtg acgatggtca
     5101 tcgggaacac gaaccgcatc tccgcctcgc tgctctcccc ggccgccacc atgtcgagcg
     5161 tgatcgccaa cgaattcgcc gaagccaccg gcgatctcta cctgtcggcg ctgatccaga
     5221 tcgcgctcgt cctgttcgcg gtgacgatcg tcgtgaacgc gctcgcccgg gccctcgtct
     5281 atctcacgac gggcggccgg cgcgacgccg gagccgcggc gtgagcgccg ccgtgcggaa
     5341 gtccgcggag gggtggagcg accccgcgcc ggcccgcgtc cgcctgtggc gcggcttcgc
     5401 gaaccgggtc gtcctgggcg cgtgctacgc ggccgcggcg ctcgtggtgc tccccctcgt
     5461 cctcatcgtg tggcatctcg tcgcgaaggg gctgccggcg ctcaacctaa gcttcttcgt
     5521 gcacatgccg aagccggtgg gagagccggg cggaggcatg gcgaacgcca tcgtggggac
     5581 tctcgtcgtg gtcggaatgg gcgccttgat cgcgctgccg ctgggcatcg ggtcgggcgt
     5641 ctatctggcg gaatacggcc gcgggcgctt cgcgatgatg gtgcgctaca cgacggacgt
     5701 gctgagcggc gtgccctcga tcgtggtggg ggtcgtggcg tacgggctgg tcgtggtgcc
     5761 gatgggccgg ttctcggcca tcgccggaag cgtggcgctc gcgatcctca tgcttccgcc
     5821 gatcatccgg gccaccgagg agatggtccg gctggtgccg caatcgtacc ggcagggcgc
     5881 cctggcgctg ggcgcgccgc gctggaaagt gattcagcag gtcgtcctgc ccgcggcgcg
     5941 cgcgggcatc gtcacggcgt ccatgctcgc catcgcgcgc gcggcgggcg aaacggcgcc
     6001 gctcctcttc accgcgcttg gaaaccgctt ctggtcacta cggctggatc agccgatcgc
     6061 caccctgccg gtcttcatct tcgactacgc gcgcgcgccc tacgccgact ggaaccgcca
     6121 ggcgtggacc ggggccctcg tgctggtgct cctggtcgcg gtggtctcgg gggtgctgcg
     6181 gctgacggtg cggaggatcc gggtccgcta ggcgcggcgt cttcccggcg gaccgctcag
     6241 gaaccgaggg tcgcgtcgcg gatctcgtcc gcgtgatgct tgatgacgcg cgcctcggtc
     6301 acgaagatcg cctcctcggc gatgttcgtg gccaggtcgg cgacccgctc cagattccgg
     6361 ctcgccagga ccatctccag ggcgcgcggc acgatctccg gctcgtgcac catctgcgac
     6421 gagaagcgcg cgaagatctg cgtcttcagc gtgtccacct cggcgtcgtt ctcgcagacg
     6481 cgcttggcca ggggcgcgtc gtggttgagc caggccgata cggcgtcgcg gagcatcgcg
     6541 atcgtgcgat cgcccatggt cgccagatct tccggtcctt tccacggcgg catcccggag
     6601 agcttgatcg cgctctgcgc gatgttcacc gcatggtcgc cgatccgctc caggaagttc
     6661 gagacccgga tcgaggcgac gagaaaccgc aggtcgcggg cgaccggctg ctgcaatccg
     6721 aggaagtcga gacagcggcg ttccacctcc agctcgatcc ggtcgatctc gagatcctcg
     6781 gtcatcacgt cccgcgccag caccgtgtcc cgcgtcttca gcgcctccat ggccttggag
     6841 agcgccgtct cggcgcggcc cgccatgtcg ctcaatcgat cttgaagctg gtgcaggtcg
     6901 tgatggaaat gacgttccac gtcgggtccc tcgatgagtt cgggcggtcg cggtccgcgc
     6961 ccggcagcgc gacccgccgg ggcatcagcc gaagcggccc gtgatgtagt cctcggtcag
     7021 cttctcgcgc ggcgaggtga agagggtccg cgtggggccc tcctcgatcc gctcgccgtt
     7081 cagcatgaag acggtgcggt cggagacgcg cgccgcctgc tgcatattat gcgtcaccat
     7141 gacgacggtg acgccgttct tgatctccag cgtgagctcc tcgatcttgg cggtggcggt
     7201 cggatccagc gcggaggccg gttcgtccag gaggagcact tccggctcga ccgccagcgc
     7261 ccgcgcgata cagagccgct gctgctgtcc gcccgacaga cccagcgccg aacgcccgag
     7321 tcggtccttc acctcgtccc acaaggcgga gcggcggagc gccgactcca cgatctcctc
     7381 cagccgccgc cggtcgcggg tgccctggag cttgggaccg tacgccacgt tctggaagat
     7441 cgacatggga aacggattcg cccgctggaa caccatcccc acccgccgcc gcagcgccac
     7501 gagatcggtc cccgggctca ggatgtccga gccgtggaag aggacgtttc cttcgtagcg
     7561 gaccggcggg aagagatcgt tcatccggtt gaagcagcga agcagcgtcg atttgccgca
     7621 accggacggg ccgatgatgg ccgtgatccg gttctgcccg atctcgagat cgaccccttt
     7681 cagggccgca tggcttccgt agaagaggga gagcctcttc gtctggagga tgggggcggc
     7741 cgaggcgtcc tcacgttcga tgggggcggg cgcggggctc acgtcggaat cgggctcctc
     7801 gggaacgagc ggtacgagcg gtgcgcgcgg cccattcggc cgggtgcggc gctccggaag
     7861 ggtggcggtc gggtccatcc ggatcgagcc tagacggccg cggcgttcgg cgtgtgtcgg
     7921 ccttgttacg tttccgttac gcgccgctgg ggcccggtcc tcttggctcc ggtccgggac
     7981 cttcccgcgg cacctcgacc cagaacgtgc ttccctgtcc ggccgcgctc tccaccccga
     8041 ccgttccgcc gtgcagctgg acggcgtgct tcacgatgga gagccccagg ccggttcctc
     8101 ccagcgcgcg cgagcgaccc cggtccaccc ggtagaagcg ctcgaagacg cgcgcaagat
     8161 gctccgcctc gatcccgcgg cccgtgtcgg tcacgcggat ccgcaccgaa tccccctggt
     8221 cctccacggc gacttcgacg gtcccgcgct cggtgtactt gaccgcgttg tccatgaggt
     8281 tccggagcgc cagctcgagg cgcttccggt ccacgcgcgc gcggatgccc gcgcggctct
     8341 ccagcgagag cttgaggccg cggcgcgcgg ccacgtcttc gaacgtcgag gccaggtcgc
     8401 gcacgatctc gccgagatcc cagtccttga ggtcgagcgg cgcgtcgggg cgctccaggt
     8461 cggccaggtc gaggagatcc tcgaccagcg cctccagccg cgaggcctgg tcccggatcg
     8521 tctccacgaa gcgcgtccgg tttccctcgt cctcgagccc gcccgagagg agcgtttccg
     8581 cgtagccgcg gatcgaggtg agcggcgtct tcagctcgtg cgacgcgttc gccacgaact
     8641 cgcgccggac cgccgccatc gccttcaccg gccccaggtc ttccaggctc acgagaattg
     8701 tctccggctc ggcgcccgcg taggcgccga tcggcgaggc gcgcacctcg taatcgcggc
     8761 ggtccgccag atagagcgtg aggtcgcgca cgagcggttt ccggtgctcg agcgcgtgca
     8821 gcgccatctg ctccaggacg ggctggcgcg tcgacgaaag gcgcgggtcg cgcggcggat
     8881 ggtccagctc cagcatctcc cagaaccgcg cgttcgcgtg gcggatcgag aggtcggggg
     8941 cgaccagggc caggcccaga ggaacgttgc cgagaatcgc ccggctggcg ttccggccct
     9001 gctgcagctc cttgagcttc gattcgaagc tgtcggcgat atcgttcagg gaacgcgcga
     9061 gccgcgcgag ctcgtcgtgg gcgtccacgg gcgcccggcg cgagacgtcg ccggcggcca
     9121 tccagctggc ggcgtcggcg atgtgggcga agcggcgatt gagggaccgg aaggggagcc
     9181 atcccgcacc gagcgccacg gcgaggaccg aggccacgat cccgatcgcc cccccggcgc
     9241 cgaccggctt cagggcgttg aggagcacgc cgaccgcgca cgccgcgagg aggagcgcca
     9301 ggagcagcag gaaatagacc cgtcccgtga gcgatcgcac gggacgtagc ctaccactct
     9361 ctcagccctt cgggacctcc gcggcgtagc ccacaccggt aacggtccgg atcagtcccg
     9421 cgtccttccc cagcttggcg cgcagccgcc ggacgtggac gtccaccgtg cgggtctcgc
     9481 cgaagtagcc cagcccccag accttgtcga ggagctgctc gcgggagaag acgcgtccgg
     9541 gacgttccag gagaaactct aagagtcggt attccgtggg ggtgaggcgg agctcgcgct
     9601 cgcgcacgaa gacctgatgg cggtcggggt cgaagcgcac cggtccgatc acggtggacg
     9661 cctggcgctc ctcgccgcgc gtgcgccgga ggatggcgct gacgcgtgcg accagctcct
     9721 tgggcgagaa cggcttggtc aggtagtcgt cggcgccccg ctcgaagccc accagcttgt
     9781 ccatctcggt gccgcgcgcg gtcagcacga gcacggggag ccctttggtg cgcgcttccc
     9841 cgcggatggc gtccaggact tcgagcccgc tcagcttggg aaggaggaga tccagcacga
     9901 cgaggtcggg aggcgacttg aagatgcggt cgaggcccgc ggcgccgtcg ttttcggcaa
     9961 tgacccggta cccctcgcgg cccaggttgt agctcacgag gtcacagatc tcggcctcgt
    10021 cgtcgacgac gaggatggtg gagcgcgcag tgcccggcac gttgtgggct cccagtctag
    10081 cacgggcgtt cacgaagggc agtctggctg cccccggtta ccgggtcgcg tcgaaaccgt
    10141 gtcgaatggg ttacgaccgc accgggaccg acggacacgg cttcccccat ttcccactcg
    10201 cgcattgact tcacgggacc ccttccggta gtctgcccgg tcttgaccga gccacaaccg
    10261 gattacaacc ccgtgcgaaa ccaggaaacc agaggaccga cgtgacgcag agcgatcgga
    10321 acaaactctt tctgaccctc atcggcctcg cggccgcgct ctacaccctc tggcccacgt
    10381 tccagttcta cgccctgtct cccgccaagc gggcggaggt gctccaggca cgccccgcca
    10441 ccgcgaccaa cgacgccgat cgcgaccgcc tcaccaagat ggccaagctc cgcgaccggt
    10501 cgatcaagct ggggctggac ctccagggcg gcctctacct cctgctcgag gtcgacaagt
    10561 cgaagctggg accggccgag gccaagggcg ccgtcgacca ggcgatgcag atcatccgga
    10621 accgcatcga ccagttcggc gtggccgagc cttccatcca gaagcagggg gataatcgca
    10681 tcctggtgca gcttcccggc ctcctggacc aggaacgcgc caaggagctg atcggacaga
    10741 ccgccctcct cgacttcaag atcgtgaaga acgacgacga ggtgcgcgcc ctcttcgacc
    10801 ggatcgacca gtacttcacg cggaagatgg cggccgcgac gggggccaag cccgattcga
    10861 cgtcccaccc ctggacctcc cgcttcctga ccgtcgccca ggcgggcgag tcgttcgccc
    10921 tgccggagaa cgaggcggcg atcgactcga tgaagaatca gctcctcgcc gactcctcct
    10981 tcaccatgga cgcgaccctg gcctgggacg cgcatgaagg ggcgcacgag agccgcaccg
    11041 gccgctccct gtacgtgctc agcaaggagc ccctgatgca ggggaaggaa gtggcgagcg
    11101 cgcagatgcg gatggacctg gaccaggagc gccccggcgc ccccggggtt tcgttcacct
    11161 tgacgggccg aggcggcgcg gtgttcgccg acgtcaccgg cgccaacgtg ggccggcgcc
    11221 tcgccatcgt cctggacaat cgcgtgcagt cggcgcccaa catccacgag cggatcccgc
    11281 gcgggcaggg ctcgatcacc gggtccttca cggaggaaga ggcgcagaac ctctcgatcg
    11341 tgctccggtc gggcgccctc ccggccccgg tgaacatcgt cgaggagcgc gcggtcggtc
    11401 cctcgctagg ccaggactcg atccagaggg gcatcatggc gggagcgatc ggcaccgtcc
    11461 tcgtggtcgt gttcatggtg gtctactacc gcatgtcggg gatggtcgcg gtggcggccc
    11521 tgctcctcaa catcatcggc cttctggcct gcatggccgc gttccatgcg acgctcacgc
    11581 ttcctggcat cgccggcatc gttctcacga tcggcatggc cgtagacgcc aacgtgctca
    11641 tcttcgagcg catccgggaa gagctgcgcc acaagaagac ggttctcacg gccatcgagc
    11701 tcggctactc gcgcgcctac cggacgatcg tggacgccaa tgtgaccacg ctgctctcgg
    11761 cgctggcgct gatgtggttc ggcaccggcc ccatccgcgg gttcgcgatc acgctgtcga
    11821 tcggactcat catcaacatg atcaccgccg tcggcgtctc caagatgatc ttcgacgcct
    11881 ggtcgctccg gcggaaggtc agtacgatca gcatctaggg gagaatcgtg ttccagatcc
    11941 tgcacggctt gaatgtcgat tggatgaggc gaaggcatat cgcctacgtt gtatccggac
    12001 tctcgatcct cgcctccatc gcctcgctcg tcatgcatgg cggcccccgt tacggcgtgg
    12061 atttcacggg gggaacgctg atcgaggtgt cgatccagcc tccgccgtcg gtggacgacg
    12121 tgcgccgcgg catcgaccag gccgggttca agggggccga gatccaacgt ctcgaacgtc
    12181 ccgatctctt attgatccgc atgcaggtgc aggagggccg gccgagtccc gcgccggcgg
    12241 tgaggcaggc gctgctggcc gcacaccccg gatcgaccgt ggacgtccgc cgcgtcgaga
    12301 acgtcgggcc gcgcgtcggc ggcgagctcc gcgcggcggc cgtcaaggcc gtcttcctgg
    12361 cgctgggtat gatcctggtc tacgtcgcca tccgctatga ctggcgctac tccatgggcg
    12421 cggtggtcgc gctcttccac gacgtcttca tcaccctcgg cgcggtttcc atcttcaaca
    12481 aggaagtcac gctcacggtc gtggccgcgc tcctcaccat cgccgggttc tcgatcaacg
    12541 acaccatcgt cgtgttcgac cggatccgtg agcggcgtca ggcgctccgc cgcgagccgg
    12601 ccgagaaggt gatgaacatc gccatcaacg agaccctgag ccggaccatc atcacctcct
    12661 tcacggtcgt gctcaccgtg ctcgcgctct tcttcctggg cggcgaagtg ctccacgact
    12721 tctccttcgc catgctcgtc ggcgtgatct acgggaccta ctcgtcggtc tacgtggcga
    12781 gcgcgctcgc cctcgagacc cagctcgcgc tcgagcggcg gacgccggcc cggaagcggt
    12841 aggaagctcc cgatgcgacc cgcggcgctc ccggcgcgaa ccgcggcgat gctgctcctg
    12901 gccggatcgg ccgcggccgg gccctcgctc tgccgcgccg aagcgccccc gcccccgccg
    12961 cctccggtct cgacgccgat cctcgcaccc gattcccgcg ccgaagcgca tcccgcggga
    13021 ccgaccgcga ccccgctgca gctcttcgcc gccgccgagg aggcgtgggg ctcgggggac
    13081 gccgaggggc tcgcctcgct ggtggacacg acgcaggttc ggatcggtct gaagccggga
    13141 tccaccccga cggccgcgat gacccggagc gccgcggcct tcctcttcca ggaccagctt
    13201 cgcctggtga cgacgcagtc gtttcaggtc acccggatca acgtggggaa cacgtccgcg
    13261 accgcgaccg cccgctggtg gggcaactgg ggcgggcgcg aggggaatcg gaagctcacg
    13321 gtgaccctga gcgcggtgcc gctcgcgggg cgctggtacc tccgcgaagt ccgggtgaag
    13381 ggctgagcgg caccttcggc agattgggcg aaggcgcacc gccgcggcca cgcgaatgaa
    13441 cgacgccgac gtcctctaca cctccgacct tcacggcagc cgttcccatt acgaggccgc
    13501 cttccgcatg gccgccgact tgggcgtccg cgccgtcgtg ctcgggggcg atctcgcgcc
    13561 gatgggcgat cccgccgcgc agcgggagtt ctttcagacc ttcctgattc cgcttcttcg
    13621 cgagcgcctc gccgccccgg gagcgccccc ggttttctac atcttcggaa acggcgactg
    13681 gcgcgcgaac gaggccgcgc tcgaggaggc gcggctgccc ggcctccatt acgtgcacgg
    13741 gcgcgccgcc cccttcctgg acggcacctg gatcgccggg cagaactgcg ttccgccgac
    13801 gccgatccgg ctgaaagact gggagcggtg ggaggtggtg cccggagcgt cggggcgtcc
    13861 ggacggacac cggagcgctc cggacggaac gctccacgac ttcacgttcg tggggcgcga
    13921 gcgggaggag accctcgaag ccgagatgga cggcctggag acggcggtcc ggggagcgac
    13981 cggatccgcg gaggccgccg ccgtggcccc gcaggcgctg gtctgcgtgt tccacggccc
    14041 gccccacggc acggccctcg atcagatcgc ggggggcacc cacgtcggtt cgggcgcggc
    14101 gcgccgcttc ctccagcgga tgcgcccggc gctctcgctg cacgggcaca tccacgagtc
    14161 gccctcggtc tcggggcgct tcgccgaccg cgtcggcggg accgtctgcg tcaacccggg
    14221 gcagaccccc gcggcgttcc acgccgtctc tttccggatg gccgatatcg cgggcaccct
    14281 gcgccatacc ctgcacggcc ccgcgaacct cgcggaacca cgctaaccgc ctgcggttta
    14341 gctgaaagga agcccggccg gggagacctc tggtatcctt tcgtggccga agtggacgac
    14401 cgcgccgacc cgaccctgga cgaggccctc ttgccgcaag atctcacgct atcccaatgg
    14461 tttagggtcg tccgatgggc aggccggggg gccgtcgcgg gcgctattgc cgcgattctg
    14521 gctgcggccc tcgcgggccc ggcggccggg cagggccccc cagccagcgc cggggccgac
    14581 ctgaccgggg ggttctccgt agcccgcctc aagtacacgg gcgggggcga ctggtactcc
    14641 gacgagacgt cgctcaagaa cctccttagc gccctgaagg cgcgagggga catccgggtg
    14701 tcgcagcaga aggaggcggt ggtcacggcg accgaccccg atctctggaa ctaccccctc
    14761 ctgttcgtga cgggccacgg aaacgtgaag ctgaacgacc aagaagtggc ctcgctgcgc
    14821 cgatacctag accagggcgg gatgctctgg ggggacgata acttcggcat ggataagtcg
    14881 tttcgcgagg cgattcagcg cgtctacccc gagtcgccgc tcgtggaact tccgttcaat
    14941 cacgagatct tccggcggcc gaacccgttc ccgagcggcg cgcccaagat tcacgagcac
    15001 gacggcggtc cggcgcgcgt cttcgccatc atgcggggcg ggcggatggt cgtcctctac
    15061 acgttcgaca ccgatatcgg caatgggatc gaggacgccg gcgtgcacga cgatccccag
    15121 gccaagcgcg acgcggccat gcgcttcgcg atcaacatcg cgacctatgc ggtgagccac
    15181 tgaagggccg gttccccatc atgaggggca atgactccat cgggcgatag ccttcatgcg
    15241 accctgagcc gcgcgcggcg tttcctggtc tggcgcgcgg tggagcaggc cgggctggcg
    15301 ctcgtcgtgg ccgcgatcct ggtcgcgctg atcgcgcttg gggtggcgct cgccacgccg
    15361 ctccatcggg acgaattcgt gctgatccgc ttgggccttt tagggtcggg ggcgctcctg
    15421 ctcctgttcg cggcggcccg cgtggtgctg gctcccgcgc gcctggagga cgcggcgctc
    15481 gcgaccggcc ggctcctggg cgagcgggac gacgagctcc tcggcgcgct cgagctggcg
    15541 cgcgcgggag cggacgaatc gcgggattcc ggcgcccttc gcgacgcggc ggtcggcgcg
    15601 gcggccgcgc gggccgcggg cgccccgatc gcgcggctcc gccgatggga cgcgcgcggc
    15661 cgctggctcc tggcggcggg gggaaccctg gtgctcctcg gcgccaccgc ggtcgccgga
    15721 ggcgcccgga cgcctgccgt gctccgccac atcgccgatc cccggaccgc cccgatcgcg
    15781 ccgatcgcga tccgcgtcga gccgggcacg cgcgacatcg agggcggtga gtcggtcgtg
    15841 atccgggcct acgtgcgcgg ctcggggcag cgccccaagc tggaagtcga ggaccgcggc
    15901 gactggaacg agacgtcgct ggacgaggcc gaggacgcca cgggcgtgaa gtcgggcgag
    15961 cgcgcgtggt cgcttctcct ccgcaatctc aaggacgatg ttcgctacaa ggtgcgcgcg
    16021 agtggccagg aatccccggt ctacgcgctc accgtgcgcg acctgccgcg cgcgaccggc
    16081 taccgcgtcc gctacgacta tcctccgtat accggcctca agtccgaaga gagccacgcg
    16141 gtgaccggcg acctggccgc gccgcgcggc acgcgcgcgc gcctggtcgt cacgctgagc
    16201 cggagcgtcc gccgcggggc gctgctcttc gagggcggta cgtcggtccc ggccgaaccg
    16261 ggggagcgtc tcgccgcctt cacggtgccg atccgaggcg acgaccggta cgccgcgcgg
    16321 ctcgaggacg cgcgcggccg ccgcgtggac ctgggaccgt tcgaggtccg cgccattccc
    16381 gaccggccgc cgaccgtctc cgtgctctcg cccggctcgg tcgaggacgt ggggcgcgac
    16441 atgacggcga cgatcatcgc gggcgcgacc gacgactacg gcgtgcggaa gatcctgatc
    16501 cgctacaagg tgcgcgacgg ggccgagaag accgaggtgc tgcacgaaga gaagggaacg
    16561 gcccgcgagc tggccgtgcg ctacacgtgg gcgctccaga gctactcgct cctgcccggc
    16621 gaagaggtcg agtaccggat cggagcgatg gacggcaacg cggtggacgg accgcagacc
    16681 acctggtccg acgcgcgcct cctccgcttc ccctccgctt ccgagatcct cgcctcgatg
    16741 tcgggcgagc gcgacgagac gatccactcc ctcgaggatg cctggcagga cgcgaaggac
    16801 ctctcccaga aggccgagga gctgtcgcgc gacctgggcc gcacccgcga gatgacgtgg
    16861 gagaagcagc aggaggcgca gaagaccctg gaggggcagc agcagctcca gcagaagatc
    16921 gaccaggtag ccgacaagct gagccaggac gcggagaagc tgagccagtc gcacgcgctg
    16981 aattccgagc tggcgcagaa gatccaggag ctgcacgaga tcctgagcca gatcaaggac
    17041 cagtcgctcc tccgctcgat ccagaggctc caggacgcga tgaagaatct ctcgccggag
    17101 cagatgcagg ccgcgctcca gaatctcaag gtgaaccagg agcaggtcat gaagagcctg
    17161 gagcggacga tcgagatgct gaagcagatc cgcaccgagg ataagatgga ggccgcgtcg
    17221 gagcgcgccg ccgagatgga gcgccggcag atcgcgctca acgactccct ctcccgcgcg
    17281 aagaagagcg aggagacgaa gtcgctcgcg aagagccagc gcgaaaacga gaagctgagc
    17341 gccgaggagc gcgccgcgct cgactcgctc gcggccgatc tcaaggcgat ggaccccgag
    17401 gcgtcgaagg aatcggagca gctcgcggac gatctcggcg aacagggggc caagcccgac
    17461 ttccagcacg cctccgagtc gatggagcag ggcgatcgcg agaactcgcg ccaggccgcc
    17521 gaacggctga agcagcgcct gacgcagctg cgccagggcg tggacaagat gcgcgagaag
    17581 ttccagaaca agcggaagaa cgagctcgcg aagaagatgg aggacgccgc gcaggacctc
    17641 ctcgacatcg gcaggctcca gcagaagatg ctgaacgacc aggagagcag tctctccgag
    17701 cgcgccgaaa cccagaaggg tctggaggac gcgaccacgg ccgtcacgaa gcggatcgcc
    17761 gacatcggca agcagacgct ccacatcacg ccggacatcg ggcaggcgct gggacgtgcg
    17821 ctcacgaacc aggacaactc ggtcggccgc tactcggagc aggacctggg cggcgggctg
    17881 atggccggga aggaagcggc gatcgcgctg aaccaggcgg cggccgggct cctccggagc
    17941 aagcagtcga tgcagagctc ctcctcgtcg accggcttcc aggaagcgat ggaccgcctg
    18001 cagagcctgg ccggcgagca ggagcagctg aacagccagt cgatggggat gatgccgggc
    18061 gaagggcagc aggaggggag ccaggcgaac cggttgagcg aaggggaggg gcaggcgctc
    18121 tcccgcatgg ccgccgagca ggaggcgatc cggcgcggcc tggaagagac gatgcagaag
    18181 ctgggcaagg gcggcggcac gctcgggaac atgggcgacg tggcggacga aatgaagaag
    18241 gtggagcagg atctccgcgg cgggcggctc aaccaggaga cgacggagcg gcagcagcgg
    18301 atcctgagcc ggctgttgga cgcgccgcgc agcgtggaga agcgcgacta ctcgcgcaag
    18361 cgccagtcgc gccccggcgt agacgtcgtg cgctcctcgc ccggggcgct ctcgcccgag
    18421 ctcctacggg cgcgcccctc actggcggcc ctgctcgcgc gcgcgggacg cgatccggtg
    18481 gccccgcgct atcgcgcgct cgtggacgag tatctccagt cggtgctggg cggcgggaag
    18541 cccaagtgac ccggcattcc atcgggaccc gccgcgcggg ccgcgccgcg atcgcgctcg
    18601 cgtctgccgc cctgctcgtg accgcggcgt cggggagcgc cggcgccaag ccgcgccccg
    18661 agcccccggc caaggccgag ccgcgcgtcg caccccgcgt tccccagccc cttccgctcc
    18721 tgaaccgcga cgaccgtctg cccgagggat accaggatct cgtgcggcgg ctgatggcgc
    18781 gcggccccgg cgacaccctg ggcgacggcg tgttcacgct ggccgagggc tcgttccacg
    18841 cgggggcctt cgacgaggcg ggagggcgct acgccgattt cgcgcagcgc ttcccgcgga
    18901 atctccgcgt gaacctggcg ctcgagcggc tgctcctcat gaaggacggc cgcgacttcg
    18961 gcgacgagcc gctccggatc tacgcgcgcg ccgacctcat gcgcgccgaa ggccacgccg
    19021 actcggccga gaccgcgctc accgccggcc tcgcgcgcta tccgggcacg aagctgcgct
    19081 accacttccg ctacgcgctc gcggagatcg cgcgcgacaa gggcaaccat gccgcggcga
    19141 tcgagcaggc gctggcggtg gccgacacgt cggccggctc gcgcctggcc cccgccgcgc
    19201 tcaagctggc gggggatgag acgctcgcca tgggcgggcc gccggagcgc gcgtccggct
    19261 actaccagtc gctcctggag cgcttccccg attcgcccct cgcgccctcg gtgcgcgagc
    19321 ggctgctcct cctccgaaag aagatgcagc tgtgagccgc gcgcgccgat ggctcctcct
    19381 tctcgtcctg gcggtggccg cgctcggcgc ccttcccggc gcggccggcg cgcggctcct
    19441 ggtgccgatg gacgacaagc aggccgacca tctccgcgcc tacgggctca cctattgggc
    19501 gctggagcgc gggatgcatg gggagtggct gctcaactac cgcggcgggt cgttcctgct
    19561 gcccgacaac gcggcgctcg cgaccgaggc ggtggtgcgc ggcgtcacgg tggagccggt
    19621 ggacgaggcg atggtggcgc ggatcatggc cgagatcgcg gacaacaaca tggagtcggt
    19681 gaagctggag accgcgccgc ggatcgccgt ctacgtgccg cccagcgcgc ccccctggga
    19741 cgacgcggta cagctcgccc tggattacgc gcagatcccc tacaccaagc tctgggacgc
    19801 ggaagttctc gacggaaagc tcgcccagta cgactggctg cacctgcacc acgaggactt
    19861 cacgggccag tacggcaagt tcttcgcggc gttcgcgacg accgactggt accggcggca
    19921 ggtggccgag aacgagacga tggccaagag ccgcggcttc agcaaggtgt cggagctgaa
    19981 gaaggcggtg gcgcgcacga tcaaggcgta cgtggcggcc ggaggattcc tgttcgcgat
    20041 gtgctccgcg accgacacgt tcgacatcgc gctcgccgcg aagaacgtgg acatcgtcgg
    20101 gcccgaatac gacggcgacg gagccgaccc gcgcgccgac gccaagctcg atttcggcga
    20161 atgcctcgcc ttccagaact tccagctgga cgagaacccg atggtctacg agttctccaa
    20221 catcgacacg acgaaccgcg cctccctgcg cggccagcgc aacgacacct tcaccctctt
    20281 cgacttctcg gcgaagcagg atccggtgcc gtcgatgctg gtgcaggacc acgtggccgt
    20341 ggtgcccggg ttcatgggac agaccaccgg tttcgagaag tcgctgatca agcccggcat
    20401 cacggtcctc gccgagacgc ccggcgccga cgacgtgaag tacctgcacg gccattacgg
    20461 caagggaacc tcacgttcta cggcggccac gaccccgagg actaccagca cgccgtgggc
    20521 gatccgccca ccgatctgtc gctgcacaag cactcccccg gctaccggct gatcctgaac
    20581 aacgtcctct tcccggccgc ggagaagaag gagaagaaga cctagccggg ccgcgggcgg
    20641 tttgctacgc tccgcccctg ccatgtacga cccgagccgc gagtacacga ccagcgttct
    20701 catcgcgggc gtcaccggcg cgatcgggcg ggcggtggcc cgcgacctga tcgtgaacgg
    20761 ctatcgcgtc tacggtctca tccgggacgc cgaatcccgc gaccgccttc cctacggcgt
    20821 cgtcggtgtc gtcggcgacg tccgcgctcc cgagaaatgg gaaacggcga tcgagcggac
    20881 gcaggtcgtg gtgaactgcg ccttcccgcg cgacctgtcc accggcgccc tcacctacga
    20941 agacgccgag cgcgaagcgg acgagctggc cgcgatcctg gatcggctct gcgacctggt
    21001 ccggcgacgg aagcgccgcc tgatccacac cttcggcgcg ctcctctacg aacccgacgc
    21061 gaacggatgg gtgcgcgaga cctcggccat cgcctccggg cggggctatg gggtgcgcca
    21121 tcgtcggacc tatcccgtct tcgagaagca ccggaagaag gggctcaagg cgatgagcgt
    21181 gaacccggcg ttcgtctatg gacgcggcgg ctggttcgaa tacgggctgg tccagccgct
    21241 cgtgcgcggc caggcggtcc tgatcggcga cggatcccag acgatgcact acgtggccgc
    21301 ttccgacgcg gccagcggat accggctcgc gatcgagcac ggcctggacg gcgaggatta
    21361 cctgctgggc gacgatcgcc ccaccaccct cggcgaattc acgcggctgg tcgcgcgcga
    21421 gctgggcgtg gccgagccgc gctcggtccc cgaggaagag ctggtggcgc agctcggcgc
    21481 ctggaacgtc gacgcctaca cgttctgccc caaggtggat tcgaccaagg cgcgcgagcg
    21541 cctcggctgg accccgcgct atccgactat cgaagaaggc gtccccgccg cgatccgcga
    21601 ttacaagcgc gcgcagctcc aggcggcatc cggataatcc gcatgcggct gcgcccagaa
    21661 cgccgccgct tcgccgtacc gcttcgcgcc gccctcgtcc tctcgctcct ggcctcctgc
    21721 gctcccgcgc ccgccgattc ctggtggcgg cacgtccgct ggctggccga cgacgcgcgc
    21781 caggggcgcg agaccggaag cccgggctac ctcgccgccg cgcagtacgt cgcgcacgag
    21841 ttcgagacgg cgggcgcccg ccctgcgggg accagcgggt acttccagcc ggtcgcgttc
    21901 atcgcccgga ggctcgtcga gcccgagtgc ctcgtcaccc tggcgcgagc cgatcgcacc
    21961 gatacgctcg tcctgggcga ggatgcgctc ctctcctcgt cgatcgacgc cgccgattcc
    22021 gtggacgctc cggcggtgtt cgcgggccac ggcctggagc tcccggagca ggggctggac
    22081 gatctcgccg ggctcgatct gcgcggcgcg gtggtcttct atctccgcgg cgcgccgcgc
    22141 gaggttcctt ccacggtcgc cgcccacgcc cagtccacgg gcgagcgctg ggcgcggctc
    22201 cgcgagcgcg gcgcgatcgg aatggtcacg atctccaacc ccaaggcgcg ttcgatcccc
    22261 tgggagcggg cccggctgtc ccgcctggag gagggtttcg cgctcgccga ttccacgctc
    22321 gacgaaaagg gtgggatgcg gttttcgatg tacttcaatc ccgaccgggc cgagcgcctc
    22381 ttccagggaa ccggacacac cttcgccgag atgctccagt tggcggatca gaaccggccg
    22441 ctcccccgct tcaccctccc cttccgcatc cgcgcgcgcg tgcggatgac ggcccggaac
    22501 gcggagagcc ccaacgtcgt cggcgtgatc ccgggtagcg atcccgcgct ccgggacgaa
    22561 tcggtcgtgc tcacggcgca cctcgatcac ctggggatcg gggcgccggt cgacggggac
    22621 tccatctaca acggagcgat ggacaacgcg agcggcgtgg cgacgctgat cgagatcgcg
    22681 catgccctgc gcggcgggcc tccgccccgg cgctcggtcg tgctcctcgc gctgaccgcc
    22741 gaggaggagg gccttctcgg gtccagctgg ttcgcggcgc acccgcccgc ggccgtgggc
    22801 cgcatggtgg cgagcctcaa cctcgacatg ttcctgccga tcgcgcccct tcgttccgtg
    22861 atcgcgtacg gcgagaggga atcggatctg ggggactggt tccgcgccgt cgcggattcg
    22921 gcgggcttga aggtggaacc cgatcccgag cccgcgcaga actacttcat ccggagcgat
    22981 cagtacagcc tggtacgcgc cggagtgccg tcgctcttcg ccgacatcgg cgccggagga
    23041 gactcgacgc tcgtacggcg gttccagctg tggcgcaaga accgctacca cgcgccgtcg
    23101 gacgacacgc gccagccgat cgatttccgg tcggccgacg atttcaaccg gctgctcctg
    23161 cgcttcaccc gggacgtcgc gaatcggccg gaacgagcgc ggtggaaggc cgactcgttc
    23221 ttccggcgct accagcgcgt tccgagctag aagccggtct cgctcgcgac cacgagcgtc
    23281 gtgcggagcg gggacaggtt gaccgtgttc tggccgccga gcagcgactt ggccgccttg
    23341 atcatctccc ggtccccgga gatgacggag accccgacga ggacgccggt ccccgggaaa
    23401 cgggacgtca ctccgcctcc cccgttcacc atccagttgt cgtagtcgag cttgtaggcg
    23461 ctcttgaaga agaccatgcc gcgcggcagg tagggcgtga gctccaccgg cacgacgccg
    23521 gcctgctcct cggggggcca ctgctggtgg tcgccgaaat acaggtacac cgccgcgcgc
    23581 acggcctgga tgtcggcgac ggcctgggct gcgcgcacgt cgcgcatgaa gttcatgtag
    23641 ttggggatgg cgatggccgc gaggatgccg accaccgtca tggcgacggc gagctcgagc
    23701 atcgtcagcc ccttggagaa tcctcggagc accccttccg gctccttccc tggcgattgt
    23761 cggcgcttcg tgcgacggtg ttccgcgctc aacgtatcgg cgtccgggag ggcgttcttg
    23821 aaggcgggaa gctgctcggg ggagcggtgt tgccgcttac gggcccatac gaagggggcc
    23881 gccgcccccg gcggccccaa gcctcttacg gaggggggaa tcctcaaccg gcgcctccgc
    23941 cggcccggtg gggggtccgt tggggcccgg gaggccggga aggccccccg ggcgaaacgt
    24001 catgctgggc ggccgcgcta gatcggcagc caataggtga tcttggcgag cagcgtgttc
    24061 tcgggctcgt tccggaacag ggtgcgcgtc ccgaggccgt tctcgaaccg gttcggatcg
    24121 atcgagaaca tcgaacggtc ctcgaagcgc tcgcggctct gcttccagac gaggaagaag
    24181 gtgctccccg ggcggtactg ccaccggtag accacgttca cgttcatcga cgagtagctg
    24241 aaatcgttgt cgcggaaatc gtagggcgtc gtggggttgt tgctcgcgtc cacgttgtgc
    24301 ccggcgtagt tcacgaagtc gtagctgtcg gcccgcgcca gctcccgcgc ccgggagtac
    24361 ttgcccaccg tcaggaacgg ctccgcgtag atctcgagcg actggttgcg cgtgaagaga
    24421 acgctggatc gcagcgtgac cgacgccgtc tgctggtgaa tgtccccgaa gatgtaggag
    24481 attcccccga tcccctgccc gccgaagatc gagacggggc gctggtctaa gtactgcgtg
    24541 tcgtcgtagc g
//
LOCUS       DASSLZ010000351         6557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_746608, whole genome shotgun sequence.
ACCESSION   DASSLZ010000351 DASSLZ010000000
VERSION     DASSLZ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746608"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            461..1096
                     /locus_tag="VFM00_13325"
     CDS             461..1096
                     /locus_tag="VFM00_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499273.1"
                     /translation="MTRFLPASLLLAALAALFAASPATAQYFFIDVNGDGQCDASDVL
                     GPEVSSVDVYLDTAHDATGAPVACQNGEEGENVLTICSYTFILEWDAAGSGTLSYGPW
                     SDNMGFTVPSGGKESGRMLWLARAAPYFLAPGRYMLGSLKVKVTGAPVLRFLSSTPLD
                     NTAMTSFGAQCGGRDYDNTMKLGSDFLDARGTSPADEGPRTVWGTVKRLYR"
     gene            complement(1087..2034)
                     /locus_tag="VFM00_13330"
     CDS             complement(1087..2034)
                     /locus_tag="VFM00_13330"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HET7499274.1"
                     /translation="MTEPLGDGGAPGGRPAASRAAIYWELARPFTLIAPALGMFTGSV
                     IALGAFPPVPLTPWTAARIALGTLMAAVLNAASNTLNQVTDLEADRINKPDRPVPSGR
                     VEPGEAIRLAGWLYVGAFVLASVVGPQCTLLAGTAALLTVMYSAPPFRLKAVPYLANA
                     VIALPRGVLLKVAGWSCVRDFGRTEPWAIGLVFGLFLLGATTTKDFADIRGDRAAGFR
                     TLPVILGPRRAAWLTAPFLVLPFLLIPYGLHRGILTGNPWVLDGLAALLVVWGGYVAW
                     LMLRRPDDLARTENHPSWTHMYVMMFVAQIGFALAYVYR"
     gene            complement(2036..4057)
                     /locus_tag="VFM00_13335"
     CDS             complement(2036..4057)
                     /locus_tag="VFM00_13335"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET7499275.1"
                     /translation="MRFPGFVAPLALLATAILVGITISDTPTPAQTRERVYILGFDGM
                     DPDVADRLIAQGKLPNLAWMKQHGAMRRLETTNPAQSPVAWSSFSTGMNPGKTRIYDF
                     LRRNPATYYPDFSTVTVQRGRFAMGFLPTRAPRIINNRQGTTFWKIASEHGVKTVVLE
                     APIDFPPEELQNGVLLSGLGVPDIRGTMGTFSYFATDAVNAGDTEMGGKVDRIRVDAT
                     GRARSVVHGPRNPFAQRDTEGRIPDLMVPIEFQRVRGTNAVRISLQGQVRTVRQGEWS
                     DWYKIRFTIAPMVAVNGIGRFHVMEASPELRVYLSPINFDPRRPPVPVSKPNSYSADA
                     ARRLGLYKTLGWPEDTWALNEEKIDEKVFMEDVNYSFNRQRALVLDAMDRLDPDLFVT
                     VFQATDKVSHMMWRLIDPKHPMYNRRLAAQYGGSIDRTYMRCDSLVGLFLRRAQRQGG
                     RATVLVVSDHGFSSFRRAVNINTWLVRNGYMTLSRQDPVRDRNLEDLFGRGTFWPNVD
                     WSKTRAYALALGQIYVNLKGRESHGIVPAGAEYDALKKELVEKFGALRDPDTGEGVVR
                     HVYAREELYKGPYFPEAPDLVVGFEEGYRVSWQTSLGGIPPNVIEPNERRWSADHCSV
                     DPATVEGVFFSSRPVDAQAPRIDDVAPSVLAKFGIARPVDMDGRMLGLR"
     gene            4355..>6557
                     /locus_tag="VFM00_13340"
     CDS             4355..>6557
                     /locus_tag="VFM00_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HET7499276.1"
                     /translation="MRTVAQDARGATFDVVPGAARFDSVNVGGALYERVTLPGAVVLE
                     TPGKPALPTLFVYAAVPDGMSPRLRVTAEDWSDRHGLSPLPVARQKFLADTPDKGPVS
                     ELTTDPDPAVYQSAGVYPASSAELGSGALVGSWWVAPIQVHAVRWDPRAGSYRVLRKM
                     TVRVDFVPASDGELAARPATRPNAQARAWDRVQRGLVQNYEAARAFPRRPSRAGPLGT
                     APLGSLRAGGSRFAANPEWKLSVANTGWVSVSYASLVAGGFPSGITIADVRVEERGYD
                     DAADTATVAVIPVEPRDNNGNGIFDSGDAVTFYARSLRDRVGVGNIELHYSDVNVYWL
                     TWGSTPAPRPLPIAGDMAGTAPTPTSFRDVIRLEEDHVAKMAPYFDPFAAKPEAIEYL
                     FWTSGDEPDQFTTPIPFVDPDPAQPFRIRARYQGRGTSGIVHHLDVTFRGSTGVVDTL
                     AAGFEFVDQDVWLLDTGFTIPGSDIGSGTSSYQHVGQRRGGFGQPLVPGSFAFLDVIE
                     ATYSRLYHARSNYLQFTSGGSGGLTEIHVDGFTGATQPNVEVYDVTDPLAPDSVVGVV
                     VSGTPGNWQASFRTDASTGERRFVALVPGAEVQLAQNAVTADAASNLRQPGAFGPANI
                     ARSILITPTAFRTEADRLANFRRAQGYVVEVANVDDVYDEFNGGIKSPRAIRRYLRHG
                     WQAWTPQPFYVLLAGDGSLDYKKHMEETSFDWVPTYFKFSTIPDNLGRELVA"
BASE COUNT         1022 a         2266 c         2250 g         1019 t
ORIGIN      
        1 tcgaacggta tcgggatcaa caacgtgatc agctactgga aggatttcga cacgtcgggt
       61 gcggtgaact cgaccgacct gaacatcgtg acgacgcatc tgaaccacaa ttgcaccttc
      121 ccgctgaatc cgtagaagat cggcggggct ctttggtgta atggctttgc agagcacggc
      181 cgcttggagg tcctcctccg tagcggagat caaagcgccg agcccggtct ccccctccag
      241 tattgaccgg atgcggcggg cgccctcggg acacacggat cggtaaccgg cgaggagcga
      301 cgatacgcga gattcgaccg gcttgaaaaa gggcagtgcc ccggcgggcg gcgcatgatg
      361 gcgccgcccg ccgttgtttt cggtatactg ggccggtgac ctggacccag gccgccgatc
      421 ccgtgtatcc cagagcgaac gcatgagccc cccgacctcc atgacccgat tcctgcccgc
      481 ctccctgctc ctggcggccc tggcggcctt gttcgccgcc tcaccggcga cggctcagta
      541 cttcttcatc gacgtgaacg gggacggcca gtgtgacgcc tcggacgtcc tcggccccga
      601 ggtctcctcg gtcgacgtct acctggacac cgcccatgac gccaccggag ctcccgtcgc
      661 atgccagaac ggcgaggagg gggagaacgt cctcaccatc tgctcctata cgttcatcct
      721 cgagtgggac gccgcggggt cgggcactct ctcctacggc ccctggtcgg acaacatggg
      781 gttcacggtt ccgtcgggcg gaaaggaatc ggggcggatg ctgtggctgg cgcgggcggc
      841 accctacttc ctggcgccgg gccggtacat gctgggatcg ctcaaggtca aggtcacggg
      901 cgcgcccgtc ctgcgcttcc tctcctcgac gccactcgac aacacggcca tgacctcctt
      961 cggggcgcag tgcggcgggc gggattacga caacacgatg aagctcggct ccgactttct
     1021 cgacgcgcgc gggacctctc ccgccgacga gggcccccgc accgtgtggg gcaccgtcaa
     1081 gaggctctac cggtagacgt aggccagcgc gaagccgatc tgcgcgacga acatcatcac
     1141 gtacatgtgg gtccacgaag ggtggttttc cgtacgcgcg agatcgtcgg ggcggcggag
     1201 catgagccac gcgacgtagc cgccccagac cacgaggagc gccgccaggc cgtccaggac
     1261 ccacggattg cccgtcagga tgccccggtg gagcccgtag gggatcagga ggaaggggag
     1321 caccaggaag ggcgccgtga gccatgccgc gcgccggggc cccaggatca ccggcagggt
     1381 acggaagccc gccgcccggt ccccccggat gtcggcgaaa tccttggtcg tggtcgcgcc
     1441 gaggagaaag agcccgaaga cgagcccgat cgcccagggc tccgtccggc cgaagtcccg
     1501 cacgcaggac catcccgcca ccttgaggag aacgccccgg ggcagcgcga tcaccgcgtt
     1561 ggccaggtag gggaccgcct tcagccggaa cggcggtgcc gagtacatca ccgtgaggag
     1621 cgccgcggtc cccgcgagga gcgtgcactg cgggcccacg acggacgcga ggacgaacgc
     1681 ccccacgtac agccatcccg ccagccggat cgcctccccg ggctccacgc gtcccgaggg
     1741 gaccgggcgg tcgggcttgt tgatccggtc cgcctcgaga tcggtcacct ggttcagggt
     1801 gttcgacgcc gcgttcagca cggcggccat cagggtgccg agcgcgatcc gcgccgcggt
     1861 ccagggcgtg aggggcacgg gagggaaggc gcccagcgcg atcacgctcc ccgtgaacat
     1921 gccgagcgcg ggcgcgatca gcgtgaaggg gcgcgcgagc tcccagtaga tcgcggcgcg
     1981 ggatgcggcg ggacggccgc cgggtgcgcc gccgtcgccc agcggttccg tcacgctagc
     2041 gcaggcccag catccgcccg tccatgtcca cggggcgcgc gatcccgaac ttggcgagca
     2101 ccgagggggc gacgtcgtcg atgcgcggcg cctgcgcgtc caccgggcgg ctcgagaaga
     2161 acacaccctc gaccgtggcg ggatccaccg agcagtggtc ggcgctccag cggcgctcgt
     2221 tcggctcgat gacgttgggc gggatgccgc cgagcgaggt ctgccacgag acgcggtagc
     2281 cctcttcgaa gccgaccacc aggtcgggcg cctcggggaa gtagggcccc ttgtagagct
     2341 cctcgcgcgc gtagacgtgg cgcaccacgc cctcgccggt gtcgggatcg cgcagcgcgc
     2401 cgaacttctc gaccagctcc ttcttcaagg cgtcgtactc cgctcccgcg gggacgatgc
     2461 cgtggctctc gcgccccttc aagttcacgt agatctggcc cagcgcgagc gcgtacgcgc
     2521 gcgtcttcga ccagtccacg ttgggccaga aggtgccgcg cccgaacagg tcctcgagat
     2581 tccggtcgcg cacggggtcc tgccgcgaga gggtcatgta gccgttccgc acgagccagg
     2641 tgttgatgtt caccgcgcgc cggaacgacg agaagccgtg gtcggagacc acgagcaccg
     2701 tcgcgcgacc gccctgccgc tgcgcgcggc ggaggaacag cccgaccagc gaatcgcagc
     2761 gcatgtacgt gcggtcgatc gagccgccgt actgcgcggc caggcgccgg ttgtacatcg
     2821 ggtgcttcgg gtcgatcagc cgccacatca tgtgcgacac cttgtcggtc gcctggaaca
     2881 cggtgacgaa gaggtcggga tccagccggt ccatggcgtc cagcaccagc gcgcgctggc
     2941 ggttgaagga gtagttcacg tcctccatga agaccttctc gtcgatcttc tcctcgttca
     3001 gcgcccaggt gtcctcgggc cagccgagcg tcttgtaaag acccagccgg cgcgcggcgt
     3061 cggcgctgta gctgttcggc ttggagaccg gcaccggcgg ccggcgcggg tcgaagttga
     3121 tcggcgagag atagacgcgc agctcgggcg aggcctccat cacgtggaag cggccgatcc
     3181 cgttcacggc caccatgggg gcgatcgtga accggatctt gtaccagtcg ctccactccc
     3241 cctgccgcac cgtgcgcacc tgtccctgca gcgagatccg cacggcattg gtgccgcgca
     3301 cgcgctggaa ttcgatcggg accatgaggt cggggatgcg cccttcggta tcgcgttgcg
     3361 cgaagggatt gcgcggcccg tgcaccaccg agcgggcgcg gcccgtggcg tccacccgga
     3421 tccggtccac cttgccgccc atctcggtgt ccccggcgtt cacggcgtcg gtggcgaagt
     3481 aggagaaggt ccccatggtc ccgcgaatgt ccggaacccc aagccccgac aggagtaccc
     3541 cattctgtag ctcctcgggg gggaagtcga tcggcgcctc cagcaccacg gttttcacgc
     3601 cgtgctccga ggcgatcttc cagaaggtcg tcccttgccg gttgttgatg atccggggcg
     3661 ccctcgtggg caggaagccc atcgcgaacc ggccccgctg gaccgtcacg gtcgagaagt
     3721 cggggtaata ggtcgccggg ttccggcgca ggaagtcgta gatccgggtc ttccccgggt
     3781 tcatgcccgt cgagaaggac gaccacgcca cgggggactg tgccggattg gtggtctcca
     3841 ggcgccgcat ggcgccgtgc tgcttcatcc acgccagatt ggggagcttc ccttgggcga
     3901 tcagccggtc cgccacgtcc ggatccatcc cgtcgaatcc caggatgtag acgcgctcgc
     3961 gggtctgggc cggggtgggg gtgtccgaaa tcgtgatccc tacgaggatg gcggtcgcga
     4021 ggagggcgag gggcgcgacg aaccccggga atcgcatcgt tcggagcctc ggattattgc
     4081 cggacgggag caaggtggta gatcgaagcc tagcacccgc cttcgcggag tgtcaaaccg
     4141 gccgcgcggg aaccctttgg gccgaagcgc cttgaccccc ctcgcaagcg gttcctatac
     4201 tgcggaaacg cccgtgcgcc acgttcccgg cccccgaggg tccaagccca tccgcgtcct
     4261 atccgctggc ttcgcggtca tactcgtatt cgccgcggcc tccctgtgcc tggcgcatgc
     4321 ggtcgcggcc ccgctcggcg gcgccggcca ggccgtgcgc acagtggcgc aggacgcccg
     4381 cggcgccacg ttcgacgtgg tgccgggagc ggcgcggttc gacagcgtga acgtcggagg
     4441 cgccctgtac gagcgggtca cgctgcccgg cgccgtcgtg ctcgagacgc ccggcaagcc
     4501 cgcgctcccg acgcttttcg tgtacgcggc cgtgcccgac ggcatgagcc cgcgcctccg
     4561 cgtgacggcc gaggattggt ccgatcgcca cggcctgtcg ccccttcccg tcgcgcggca
     4621 gaagttcctc gccgacacgc ccgacaaggg ccccgtctcg gagctcacga ccgatcccga
     4681 tcccgccgtc taccagagcg ccggcgtcta tcccgcctcg tccgcggagc tgggctcggg
     4741 cgcgctcgtg gggtcatggt gggtcgcgcc gatccaggtc catgcggttc gatgggaccc
     4801 gcgggccggc tcctatcgcg tgctccggaa gatgacggtt cgggtggatt tcgtccccgc
     4861 gagcgacggc gagctcgcgg cgcggccggc gacgcgcccc aacgcgcagg cgcgcgcgtg
     4921 ggaccgcgtg cagcgcgggc tggtgcagaa ctacgaagcc gcgcgcgcgt ttcccaggcg
     4981 cccgtcgcgg gcggggccgc tgggcacggc accgctcgga tcgctgcgcg cgggcggttc
     5041 gcggttcgcg gcgaaccccg agtggaagct ctcggtcgcg aacaccggat gggtctccgt
     5101 ctcgtacgcc tcgctcgtgg cgggtggctt cccctcgggg atcaccattg ccgacgtccg
     5161 ggtcgaggag cggggctatg acgatgccgc cgacacggcg accgtggccg tgatcccggt
     5221 cgagccgcgc gacaacaacg ggaacggaat cttcgactcc ggcgacgcgg tcacgtttta
     5281 cgcgcggagc ctgcgcgatc gcgtcggcgt ggggaacatc gagctgcatt actcggacgt
     5341 gaacgtctac tggctgacct ggggaagcac gccggcgccg cgccccctgc cgatcgcggg
     5401 agacatggcc ggcacggccc ccacgcccac gtcgttccgc gacgtgatcc ggctggaaga
     5461 ggatcatgtc gcgaagatgg cgccctactt cgatcccttc gcggccaagc ccgaggcgat
     5521 cgagtacctg ttctggacca gcggcgacga gcccgaccag ttcacgactc cgattccgtt
     5581 cgtggacccc gaccccgcgc agccgttccg gatccgggcg cgctatcagg ggcggggcac
     5641 gagcggaatc gttcatcatc tcgacgtcac gttccgcggc tccacgggcg tcgtcgatac
     5701 cctcgccgcc ggattcgagt tcgtggacca ggatgtctgg ctgctcgaca ccgggttcac
     5761 gattcccggc tcggacatcg gctccggaac gagctcctac cagcacgtcg gccagcgacg
     5821 tgggggtttc ggccagccgc tggtccccgg gagttttgcc ttcctggacg tgatcgaggc
     5881 gacctatagc cggctatacc atgcacggag caattacctg cagttcacga gcggaggctc
     5941 gggcgggctg accgagatcc acgtcgatgg atttacggga gccactcagc ccaatgtcga
     6001 ggtctacgac gtcaccgacc cgctcgcgcc cgactcggtc gtcggcgtgg tcgtgagcgg
     6061 gacgccgggc aattggcagg cgtcgttccg gacggatgcc agcacgggcg agcggaggtt
     6121 cgtcgcgctc gttcccgggg ccgaggtgca gctcgcccag aatgccgtga cggcggacgc
     6181 cgcatcaaac ctgcgccagc ccggcgcctt cggtccggcg aacatcgccc gatcgatcct
     6241 gatcaccccc acggcgttcc ggaccgaagc cgatcgcctc gccaatttcc gccgggcgca
     6301 aggctatgtc gtcgaagtgg cgaacgtgga tgacgtgtac gacgaattca acggcggcat
     6361 caaatcgccg cgcgcgatcc gccggtacct gcgccatggc tggcaggcat ggacgccgca
     6421 accgttctac gtcctgctgg ctggtgacgg cagcctggac tacaagaaac atatggagga
     6481 gaccagcttc gactgggtgc ccacctattt caagttctcg accatccccg acaatctcgg
     6541 gcgcgagctc gtggccc
//
LOCUS       DASSLZ010000352        15920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_746856, whole genome shotgun sequence.
ACCESSION   DASSLZ010000352 DASSLZ010000000
VERSION     DASSLZ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 15920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15920
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746856"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VFM00_13345"
     CDS             complement(<1..927)
                     /locus_tag="VFM00_13345"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET7499277.1"
                     /translation="MSVRVIVGAQWGDEGKGKIVDLLSEEADVVARYQGGPNAGHTVC
                     TGGKTFVLHLIPSGILRPSTVCYIGNGVVVDLAALEDERNAVAKQGISVEGRLFVSGS
                     AHVILPDHRLAERHNESGASGPAIGTTGRGIGPAYTDKAARMGLRVADLLEPEIREER
                     VRMLRERTLKLIGKDTPVEPIDQVLARLEKDAERVRPMVVNVSLAVSEAVAAGKRILL
                     EGAQGTHLDLDHGTYPFVTSSSAVSGGACTGVGIGPTQIDEVIGVAKAYATRVGNGPF
                     PSELAGAIADLLREEGNEYGATTGRPRRCGWFD"
     gene            1398..2252
                     /locus_tag="VFM00_13350"
     CDS             1398..2252
                     /locus_tag="VFM00_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499278.1"
                     /translation="MARLDDPGIRAAIAAQDRATPGLLGNGILGTGVSADAAGKAYIV
                     VYTEREGVRVPAEIDGIATRTLVTGRILPYALQMGTSTGNDNECASGTLGAVVVKGGV
                     DYFLSNNHVFARENAASNGERIDAPGRYDGKPKCAQTPQIGTLAAYQPINFGGSNTVD
                     CAIAQPVAGLSYTNASAGGYTPSQTTVAPSVGLAVKKTGRTSKLTHDTIRAINVTITV
                     GYSAGNAVFTGQIQTGGQFIRSGDSGSLEVTETGNNPVGLCFAGSSQASFANPIGPVL
                     QSLGVSMK"
     gene            2334..2699
                     /locus_tag="VFM00_13355"
     CDS             2334..2699
                     /locus_tag="VFM00_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499279.1"
                     /translation="MNTPRSRRWSAAALLLLALVLVGTSCTQKMPDPRNPSRQIALGD
                     TMRENLVPIEDVLRRQTPSLMTIKGVTGTGEGRADGKPVIVVYTSHITREDRIAIPGK
                     IEGYKVEVREVGDVTAPPK"
     gene            complement(2704..3321)
                     /gene="thrH"
                     /locus_tag="VFM00_13360"
     CDS             complement(2704..3321)
                     /gene="thrH"
                     /locus_tag="VFM00_13360"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF010109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HET7499280.1"
                     /translation="MIAAIDLEGVLAPEIWPHLGEEFGIPELHLTTRDIADFEELMQR
                     RVTALDRAGITLAKVQKAAHEVQPYLGSREFLRRIRRHCQVMIISDTFHEMAEPLVEH
                     LGGFNLFANRFETDAHGRIVGWKLRIRGRKAMVIEGMRRAGFKVIGMGDSLNDLTLLQ
                     NADYPVLYRPVAALREALPQATVVDGLDEALSRFLEIFRKNGAET"
     gene            complement(3894..4835)
                     /locus_tag="VFM00_13365"
     CDS             complement(3894..4835)
                     /locus_tag="VFM00_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HET7499281.1"
                     /translation="MTQRRVLVSDPLSPQAIALLRAAPGFLVEEVRGKKEEELLPMVA
                     TIDAWVVRGATKVTRRLLEAAPQLRWVARAGAGLDNIDTAAASERGIGVLNVPGANAV
                     AVAELVFGLLLALFRRIPEADAAVRRGGFDKVQGRELRGKTLAIVGLGKIGGAVARRA
                     RAFEMEVIGFDPVVSSAQARELGVEPVTFEDLFPRADILTLHAPMLPQTKRMVGAAQI
                     SRMPRGAVLVNAARGGLVDEAALVEALRSGALAGAALDVFAEEPPKGSPLLSLPNVVL
                     TPHIGAATVEAQEAVGEEIVRLLLGKMGVGATSASSS"
     gene            4863..5351
                     /locus_tag="VFM00_13370"
     CDS             4863..5351
                     /locus_tag="VFM00_13370"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7499282.1"
                     /translation="MAPRPASPVPGAPSVSIVRATRDHADLARQALTELPERPVDESA
                     LREFLDDERRYLLLAVENGLAVGSLYGYALRQPYRVEPQFLLYALDVREGFRRRGIGR
                     ALVERFGAEARGAGAYEMWVLTSASNEAGMRLYERCGMRRENPDDVMWVLSLEPTRGP
                     MR"
     gene            complement(5641..6798)
                     /locus_tag="VFM00_13375"
     CDS             complement(5641..6798)
                     /locus_tag="VFM00_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET7499283.1"
                     /translation="MKRVLFTPGPTELPHAVLEAMARPIIHHRTDEYRQLFLEATRLM
                     QEHLGTAQPVLTLSCSGTGAMEAAAINVLSPGDEAIVVEGGKFGARWATICEAYGITA
                     HKVSVEWGRVATVEEAESALAAHPKAKALFLTHSETSTGALFPAHEIARAARSRGVLT
                     IIDAVTSYGVYDLHFDSSDLDGVVWGSQKGLMIPPGLGFVSYGPRAWAAAEKARLPRF
                     YFDLRKARADLEKGDTPFTPAITLVLAANAAAKLMAAEGREGVFARHRRNAGGTRAAA
                     QALGLSLLAEVPSNAVTAVIAPVGIDSGAVVKTMEKRYGVKIAGGQNQLKGKIFRLGH
                     IGWYDEGDILRMVGAFESALIDHGWKAEPGTAAQAAQRSFREQGAVVSSVS"
     gene            complement(6845..9280)
                     /gene="lon"
                     /locus_tag="VFM00_13380"
     CDS             complement(6845..9280)
                     /gene="lon"
                     /locus_tag="VFM00_13380"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET7499284.1"
                     /translation="MIRIERDGEVFEIRDKIPLLPLRDVVIYPYMTIPLLVGRNASVN
                     AIEAAVQRDRVVFVLAQKRPEVADPASKDLYRVGTVVRVLQLFRLPDGTMRVLVEGIC
                     RGRGKKFFSEKDSMSAAVGILKDDVKQDAQLEALMRNVLASFNDYVHLNRRVPDEVLA
                     TANNITNPVLLSYTVASHLLLKVNVKQAILEEDDVLERFRLLSKTLASELEILKLERK
                     IEGQVRSQVTKNQKEFYLNEQLKAIRKELGYANEFSNEVEELVAQVKKAKMPKDVHEK
                     AIKELDKLGKMSFMSPEATVVRNYIDWLVALPWSVLTDDNGDIAAVEKILNEDHYGLK
                     KVKERILEYLAVMKLAGSLKGPILCFVGPPGVGKTSLGKSIARALGRKFSRMSLGGVR
                     DEAEIRGHRRTYIGSMPGRIVQALRKAGSRNPVMLLDEIDKLGTDFRGDPASALLEVL
                     APEQNHTFNDHYLEVDLDLSQVMFITTANSLYSIPSALLDRMEIVRLPGYLEFEKVRI
                     AKDFLIPKELKAHGLEESDLKITDEALREVIGSYTREAGVRSLEREIAAICRKVAKKK
                     AIAMSKKKARTFRVTPANVASYLGVPKFLDSQIEKRSRIGVATGLAWTEVGGDVLTVE
                     VGVLPGSGGLSMTGKLGETMRESGQAALTYIRSRAQALGLEKNFYREIDVHVHLPEGA
                     IPKDGPSAGITMATALVSALTGVPTRDDVAMTGEITLLGNVLPIGGLNEKVVAAARAG
                     VKTVIIPKQNEKDLKELPDYLKQAVQFKLVETMDQVLEIALNTGESRSIVARPAEEVT
                     PVPAPKAAQTYAH"
     gene            complement(9436..10689)
                     /gene="clpX"
                     /locus_tag="VFM00_13385"
     CDS             complement(9436..10689)
                     /gene="clpX"
                     /locus_tag="VFM00_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET7499285.1"
                     /translation="MKKRVHSPHPIRCSFCGRGQDEVSRLVSGPSVYICSECVKLCND
                     ILEGETDHAAGLPKGSLPKPAEIKKVLDDYVVGQDRAKKTLSVAVYNHYKRIYSGGAV
                     EDVELEKSNILLVGPTGTGKTLLARTLARILKVPFAIVDATCLTEAGYVGEDVENILV
                     RLLQATDFNVVNAEHGIVYIDELDKISRKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPQQKYIEVNTKDILFICGGAFEGLEKIIERRVSNKAMGFGADIKSRKDR
                     NLGEMLSLVEPEDLLRYGLIPELVGRLPVIGALDSLSKSSLVDILTKPKNAITKQYQK
                     FFEMEGVKLEFSEDGLEAVAELALKRGTGARGLRAVLEESMLDLMYEIPSRLEVVGCT
                     ITREVIEKKTPPILAFRGDRRRKEA"
     gene            complement(10712..11332)
                     /gene="clpP"
                     /locus_tag="VFM00_13390"
     CDS             complement(10712..11332)
                     /gene="clpP"
                     /locus_tag="VFM00_13390"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HET7499286.1"
                     /translation="MSLVPIVVEQSGRGERAYDIFSRLLKDRIIFIGTPIDDVVANLV
                     IAQLLFLESEDPDRDVNLYINSPGGVVSAGLAIYDTMQFIKPDVATICMGQAASMAAL
                     LLAAGANGKRSALPHSRIMIHQPLGGSQGQATDIEIYTKEILTLRTELNKILVRHTDQ
                     PLPKIEKDTDRNFFMSAEEAKAYRIVDKVITKMDLKEKKPEPVPVG"
     gene            complement(11409..12680)
                     /gene="tig"
                     /locus_tag="VFM00_13395"
     CDS             complement(11409..12680)
                     /gene="tig"
                     /locus_tag="VFM00_13395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET7499287.1"
                     /translation="MRVDVRDTGVWQKTLSIEVPAEELDADFEAVIEDYRRKAAVPGF
                     RKGKVPRSVLELQFGHSLEQDVLERAVKRSYESAVRESKLEPITFPEIGKITFDRGKP
                     LSYEAKVDVRPDVTPKNYEGLELPTRDTEVPDEAVEKALEDLRQRAAQWEDVDRAASE
                     GDALLVDYARLNAKGKPVRRSEQKDAQIELSASGLLPEFREHLVGRKAGDHVTFDVQY
                     PQEFGNEELRGRTVTFAVQVKAVRERRPRELNDQLAVELAGMRDLAELRARIRLNMEG
                     ESRLALHREQDEALAAQLVEKNPFELPESMIAEYLEDLLQRLNREGRELSDEEAERFR
                     GEYRPHAEKRLKRDLLLDALARVEKLEVSDEEIDQAIRAAAEGEMNEAETERLLRHEG
                     QRDRVRAHISERKALALLRERARAKTLIVTP"
     gene            complement(12700..12784)
                     /locus_tag="VFM00_13400"
     tRNA            complement(12700..12784)
                     /locus_tag="VFM00_13400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12748..12750),aa:Leu,seq:tag)
     gene            complement(12868..14859)
                     /locus_tag="VFM00_13405"
     CDS             complement(12868..14859)
                     /locus_tag="VFM00_13405"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HET7499288.1"
                     /translation="MPRMVEEPGARARHPFLARLSEGILLADGAMGTFLYDKGIPFDR
                     SFDALNLVEPALIQSVHREYIRAGAEVIETNTFGANRFRLAAHGVTDSPRQVNRAGAQ
                     IARNAREEVGEPVFVAGAIGPLGKPVAPLGTIPRDEALAAYREQAEGLVEGGVDLLIV
                     ETQTDLSEALLAVDAVRSVTSDLPLVVEMTYTEDGRTLHGTYPEDAVRALSAKAVDVI
                     GANCSVGPHELLEIVDRYARKGTRPIAVMPNAGLPRLVNGRFLYLSSPEYFADYAVRF
                     AEAGARLIGGCCGTTPAHVRRMHDALARRRESPSGTGTAAVPSEGRDAGADDSSRERH
                     APRGTVTVERDRDRDRPRATVEETAPSIDPAQRSRLAAKIARREFVVSVEVDPPRGIR
                     PRKMIEGATLLKTSGVDMINVADSPMARVRMSSIALATMIQSQVGIETILHFTCRDRN
                     LMGIQSDLMGAHALGIRNILALTGDPPRAGDYPNTTAVFDVDSIGLIRVLGQLNAGVD
                     LGGNSIGEPTRFVIGCAVNPSAENFDQELERFHAKVEAGAEFAMTQPLYELATLTRFL
                     EAAKKPRIPVLLGLLPLQSHRHAEFLHNEVPGIVIPDPARKAMREAGDRGIDVGIEMC
                     LDLLVEARALVEGAYLMPSFGRYEVVARVVEAVVPGARA"
     gene            14909..15697
                     /locus_tag="VFM00_13410"
     CDS             14909..15697
                     /locus_tag="VFM00_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET7499289.1"
                     /translation="MNLLVFVKQVPDTETRIQIQNGQVDVSSVKWIVNPYDEFAIEEA
                     LRIKERLGAGRVTVASLGPERVKEAIKYGLSLGADEGVWLKGDGVALGDPGSVATVLA
                     AAAKKIGFDLILTGKQGVDQDWSQVGVMLAERLDVPHVSVVVTLNVDAGAKKGTAHRE
                     VEGGAETVEFELPAVITAQKGLNEPRYASLKGIMAVKKKVIPEWTLGDLGVDAASVGD
                     AAAGVRILEALPPPARSAGRVLEGEPADSARELVRLLREEAKAI"
     gene            15697..>15920
                     /locus_tag="VFM00_13415"
     CDS             15697..>15920
                     /locus_tag="VFM00_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET7499290.1"
                     /translation="MANPVKVAVVAETKGGAVRKIALEMLAEARRLAGEAGGTVGAVV
                     IGPCSAGEAERLAQHGADRVVHVTGAALET"
BASE COUNT         2448 a         5393 c         5362 g         2717 t
ORIGIN      
        1 gtcgaaccag ccgcagcggc gcggccgtcc cgtggtcgcg ccgtattcgt tcccttcctc
       61 gcggaggaga tcggcgatcg cgcccgcaag ctcggagggg aagggaccgt tcccgacgcg
      121 ggtggcatac gccttcgcca cgccgatcac ctcgtcgatc tgcgtggggc cgatgccgac
      181 gcccgtgcag gcgccgccgg agaccgcgct ggacgaggtg acgaacgggt aagtgccgtg
      241 gtccaggtcg aggtgcgttc cctgagcccc ctcgaggagg atccgcttgc cggccgcgac
      301 cgcttcggaa accgcgaggg agacgttgac caccatcggc cggacccgct cggcgtcctt
      361 ctccaggcgg gcgagcacct ggtcgatcgg ctcgaccggg gtgtccttcc cgatcagttt
      421 gagcgtgcgc tcccggagca tccggacccg ctcctcgcgg atctccggct cgaggaggtc
      481 ggcgacgcga agccccatgc gggccgcctt gtcggtgtag gccggtccga tgccgcggcc
      541 ggtggtgccg atggcgggtc cggaggcgcc cgactcgttg tggcgctcgg ccaggcggtg
      601 gtcgggcagg atgacgtggg ccgagccgct cacgaagagg cgcccctcga ccgagatccc
      661 ctgcttcgcg acggcgttcc gctcgtcttc gagggcggcc aggtccacga cgacgccgtt
      721 gccgatgtag cagacggtcg aaggacggag gatgccggaa gggatcaggt gcaggacgaa
      781 cgtctttccg ccggtgcaga ccgtgtgccc cgcgttcggt ccgccctggt agcgcgcgac
      841 cacgtccgcc tcttcgctca agaggtcgac gatcttcccc ttgccctcgt cgccccactg
      901 ggcgccgacg atcacccgga cggacatctg ctaggcctcc aaacgaaaag accgcgagcg
      961 cgaggcgccg cggctaagct ccagtctcga ctggcggaaa cctaccagag cgggcgggga
     1021 ttcgtcaaga cagcggcagg ttgccggccc ggcgggtttt catagactct atcgggacag
     1081 tgtgttataa tgtgaccgtt ctgcttcaaa accggggtca ctgcggtatt tgttcgcgcg
     1141 ttcgaccggg agcggtcccc accaagcgct gacgcttcgg ctggccggac tcttcctgct
     1201 ggacgcgacc gattcggtcc cttcctcatc aggaggaaat ccccaccatg aagcgcccct
     1261 cccgccgttc gctgtttctt tccgcctccc tcgtcctcgc tgcgagcgcc ctttggctca
     1321 ccggatgcag caaggatggc gcgccggtct ttgcacccaa gctgggctcc tcgggctcga
     1381 gtgacttgtc cgtaggcgtg gcccgcctgg atgatcccgg cattcgcgcc gcgatcgccg
     1441 ctcaggaccg cgccactccg ggcctcctgg ggaatggcat tctcggcacc ggcgtctcgg
     1501 ctgacgccgc cggcaaagcg tacatcgtcg tctacaccga gcgtgagggc gtccgcgtgc
     1561 cggccgagat cgacggcatc gccacccgca cgctggtcac cggcaggatc ctcccgtacg
     1621 cgcttcagat ggggacctcg acgggcaacg acaacgagtg cgcctccggc acgctcggtg
     1681 cggtcgtagt caagggcggg gtggactatt tcctgtcgaa caatcacgtc ttcgctcgcg
     1741 agaatgcggc atccaacggc gagcggatcg acgcgcccgg ccggtatgac ggcaagccga
     1801 agtgcgcgca gaccccgcag atcggcaccc tggccgccta ccagccgatc aacttcggcg
     1861 ggtcgaacac ggtggactgt gcgatcgccc agcccgtcgc cggcctttcg tacacgaacg
     1921 cttcggcggg cggctacacg ccgtcgcaga ccaccgtcgc tccgtcggtc ggactggcgg
     1981 tcaagaagac gggacgcacc tccaagctca cgcacgacac gatccgcgcg atcaacgtga
     2041 cgatcacagt gggctacagc gccggcaacg cggtcttcac gggccagatc cagacgggcg
     2101 gtcagttcat ccgttccggc gactccggct cgctcgaagt caccgagacc ggcaacaacc
     2161 cggtcggcct ctgcttcgcc ggctcgtcgc aggcctcctt cgcgaatccg atcggcccgg
     2221 tgctgcagtc gctgggcgtc agcatgaagt agactctcga acgaccccgg tgcgggcgga
     2281 gcgctccggc gcttcgcccg ctttttttag cggtctccag aacgaggtgg accatgaaca
     2341 ctccccgttc ccggcggtgg tccgccgccg ccctgctcct cctcgcgctc gtcctggtcg
     2401 ggacctcctg tacgcagaag atgcccgatc cccggaaccc gtcgcgccag atcgccctgg
     2461 gagacaccat gagagagaac ctggtcccga tcgaggacgt gctccgccgc caaacgcctt
     2521 cgctcatgac catcaaggga gtgaccggaa ccggcgaggg gagggcggat ggcaagccgg
     2581 tcatcgtcgt ctacacctcg cacatcacgc gcgaggaccg gatcgccatc cccggcaaga
     2641 tcgagggtta caaggtcgag gtgcgggaag tcggggacgt cacggcgccg ccgaagtgat
     2701 gtctcaggtc tcggcgccgt tcttccggaa gatctcgagg aagcgggaga gcgcctcgtc
     2761 caggccgtcc acgacggtcg cctggggcag cgcctcgcgc agcgccgcca ccgggcggta
     2821 gagcaccgga tagtcggcgt tctggagcag cgtcaggtcg ttcaggctgt cccccatccc
     2881 gatcaccttg aaccccgcgc ggcgcatccc ctcgatcacc atcgccttcc gcccccggat
     2941 ccggagcttc caccccacga tgcgtccgtg cgcgtcggtc tcgaagcggt tggcgaagag
     3001 attgaagccg cccaggtgct ccaccagcgg ctccgccatc tcgtgaaacg tgtcggagat
     3061 gatcatgacc tggcagtggc gccggatgcg gcgcaggaac tcgcgggagc ccaggtaggg
     3121 ctggacctcg tgcgcggcct tctggacctt ggccagcgtg atccccgccc ggtccagcgc
     3181 ggtgacgcgc cgctgcatca gctcctcgaa gtcggcgatg tcgcgcgtgg tgaggtggag
     3241 ttcggggatg ccgaactcct cccccaggtg gggccagatt tcgggggcga ggacgccttc
     3301 caggtcgatc gcggcgatca tgagggcggg agtgtagcgg ccgtggccgg cggtgagaag
     3361 ggctcaggag gccggaggcg cccttggagt gtgcgggggc ggtgctgccc ggccgaaaaa
     3421 gagtgaggga ggcttgtgag gcctccctcg aagctccggt ggtctgtttg gattcgccag
     3481 tcagaccggt ccgacacagg cgaaacgggt gcgactagct acagccacct ccgctgatcc
     3541 tactgccgat accatcgtgc actggaccac ctcctttccg cgttgggaaa atgctcggat
     3601 attccgcgaa tcgtgtcaac ggaaagtcga acgcccgggc gaaaaaatgg ggggccggcc
     3661 aaggacaggg cgagagcgcc gccgagggcg ggtaaaggat agtctacggg cgggtctgct
     3721 ccaggccggc gggcaactcg ccccggggcg agttaatata taacttaact tgcgccggcg
     3781 caagttggcg gcgacgagcc tcggcccgcc cgacctcggc cgtggcaggt tgcgcggcac
     3841 cgcttgggca ggcacggcct caccaacacg gccgggcagc ctcggcgggc cgtctaggac
     3901 gagctagcgc tcgtggctcc tacacccatc ttccccagca gcagccggac gatctcctcc
     3961 cccaccgcct cctgcgcctc gaccgtggcc gcaccgatgt gcggcgtgag gaccacgttc
     4021 ggcagcgaaa gaagcggcga gcccttcgga ggctcctccg cgaacacgtc gagggccgcg
     4081 cccgccaggg cgccgctccg gagcgcctcc acgagggccg cctcgtccac cagccctccg
     4141 cgggctgcgt tcacgaggac cgcgccgcgc ggcatcctgg agatctgggc ggcgcctacc
     4201 attcttttgg tctggggaag catcggcgcg tgcagcgtga ggatgtcggc gcgcgggaag
     4261 aggtcctcga acgtgaccgg ctcgaccccc agctcccgcg cctgcgccga ggagaccacc
     4321 gggtcgaagc cgatgacctc catctcgaag gcgcgcgccc ggcgggcgac ggcgccgccg
     4381 atcttcccca gcccgacgat ggccagcgtc tttccccgaa gctcgcgccc ctggaccttg
     4441 tcgaagccgc cgcggcgcac cgcggcgtcc gcctcgggaa tcctccggaa cagggcgagg
     4501 aggagaccga acaccagctc ggccacggcc accgcgttcg ccccggggac gttcaggacg
     4561 ccgatccccc gctccgatgc ggccgccgtg tcgatgttgt cgagcccggc gccggcgcgc
     4621 gccacccagc gaagctgagg tgccgcctcc aggagccgcc tcgtcacctt ggtggcgccc
     4681 cgcacgaccc aggcgtcgat ggtggccacc atcggcagga gctcctcctc cttcttgccg
     4741 cggacctcct cgaccagaaa tccaggcgcg gcgcggagga gggcgatggc ctggggagaa
     4801 agaggatcgc tgacgaggac gcggcgctgg gtcatggcgg cgggagtgta cactacccgc
     4861 ccgtggcccc aagacccgct tcgcccgtcc ccggagcgcc ctccgtctcg atcgtacgcg
     4921 cgacgcgcga ccacgccgat ctcgcccgcc aggcccttac cgagctccct gagcgccccg
     4981 tcgacgaatc ggcgctgcgc gaattcctgg acgacgagcg ccgctatctc ctgctcgcgg
     5041 tcgagaacgg cctggcggtc gggagcctgt acggctacgc gctgcggcag ccctaccggg
     5101 tcgagccgca gttcctgctc tatgccctgg acgtgcggga gggatttcga aggcgtggga
     5161 tcggacgcgc gctggtggag cgattcggcg ccgaggcccg cggtgcaggg gcttatgaaa
     5221 tgtgggtgct cacgtccgcc tcaaacgaag ctgggatgcg cctgtatgag cggtgcggca
     5281 tgcgacggga gaatccggac gacgtgatgt gggtgctgtc gctggaaccg acacgtggac
     5341 ccatgcgcta gcgactcctc gacacacttc cgtagtactc actgacgtga tgcatcacat
     5401 cgatgaccgg catctccgcg cctcgccagt aggccttccg tccgacaatc agggcgacaa
     5461 gctcatcctc gctccgtgag tgtgccgtcg cttctccagt agtaggcagg tcctcggttt
     5521 ggagaccaac tgccgccctt tctccgatat tgatgagggc gtgtgcgagc agcagggcga
     5581 tccgggacat gcggactccc aacgcaaccg gcttgtgtat cgcagctcga ctggggatga
     5641 ctaggaaacg gagcttacga cagccccctg ctcacgaaac gaccgctgcg cggcttgcgc
     5701 cgccgtcccc ggctccgctt tccagccgtg atcgatcagc gccgattcga atgcgccgac
     5761 catccggagg atgtccccct cgtcgtacca gccgatgtgc cccaggcgga agatcttccc
     5821 tttgagctga ttctgcccgc cggcgatctt gacgccgtaa cgcttctcca tcgtcttgac
     5881 gacggcgccg ctgtcgatgc cgaccggggc gatcacggcg gtcacggcgt tggaaggcac
     5941 ctcggccagg agcgatagcc cgagcgcctg cgcggcggcc cgggtccccc cggcattccg
     6001 gcgatggcgc gcgaacaccc cctcgcgccc ctcggcggcc atcagcttcg cggcggcgtt
     6061 ggcggcgagc accagcgtga tcgccggcgt gaagggcgtg tcccccttct cgagatcggc
     6121 gcgggccttc cgcaggtcga agtagaagcg cggcaggcgg gccttctcgg cggcggccca
     6181 cgcgcgtggg ccgtacgaca cgaaccccag ccccggcggg atcatcagcc ccttctggga
     6241 gccccagacc acgccgtcca gatcggacga atcgaagtgg agatcgtaaa cgccgtagga
     6301 agtcacggcg tcgatgatcg tgagcacgcc gcgcgagcgg gcggcgcgcg cgatctcgtg
     6361 cgccggaaag agcgcgccgg tcgaggtctc gctgtgggtc aggaagagcg ccttggcctt
     6421 ggggtgtgcc gcgagggccg attccgcttc ctcgacggtg gccacacgcc cccactcgac
     6481 gctcaccttg tgcgccgtga tgccgtaggc ctcgcagatc gtggcccagc gggcgccgaa
     6541 ctttccgccc tcgacgacga tcgcctcgtc gcccggcgag agcacgttga tcgccgcggc
     6601 ctccatggct cccgtgccgg aacaggacaa ggtcagcacg ggctgcgcgg tgcccaggtg
     6661 ctcctgcatc aggcgggtcg cttccaggaa gagctgacgg tattcgtcgg tgcggtggtg
     6721 gatgatgggg cgggccatgg cctcgagcac cgcatgcggc agctcggtcg ggccgggcgt
     6781 gaagagcacg cgcttcatca cgtttcctca gaagccggag ttcgcgcgga gcgcgacgcc
     6841 gggctcaatg ggcgtaggtc tgggcggcct tgggcgccgg caccggcgtc acttcttccg
     6901 ccggacgggc gacgatgctc cgcgactcgc ccgtgttgag cgcgatctcc agcacctggt
     6961 ccatcgtctc gaccagcttg aactggaccg cctgcttcag atagtcgggc agctctttca
     7021 gatccttctc gttctgcttc ggaatgatga ccgtcttcac gcccgcgcgc gccgccgcga
     7081 cgaccttctc gttcaggccg ccgatcggga gcacgttgcc gagcagggtg atctcccccg
     7141 tcatggccac gtcgtcccgc gtcggaacgc ccgtgagcgc cgagaccaac gcggtcgcca
     7201 tcgtgatccc cgccgagggg ccgtccttgg ggatggcgcc ttccggaagg tgcacgtgga
     7261 cgtcgatctc ccggtagaaa ttcttctcca atccgagcgc ctgcgcccgg gagcggatgt
     7321 aggtgagcgc ggcctgtccc gattcccgca tcgtctcgcc cagctttccc gtcatgctga
     7381 gcccgccgct gccggggagc acgcctacct cgacggtgag cacgtcgccg ccgacctcgg
     7441 tccaggcgag cccggtcgcc acgccgatgc gcgagcgctt ctcgatctgg ctgtcgagga
     7501 acttcggcac gcccaggtac gacgccacgt tggccggagt gacccggaac gtccgcgcct
     7561 tcttcttgga catggcgatg gccttcttct tcgcgacctt ccggcagatg gcggcgatct
     7621 cgcgctcgag actccgcacg ccggcttcac gcgtgtagct gccgatcacc tcgcgcagcg
     7681 cctcgtcggt gatcttgaga tcgctctcct ccagcccgtg cgccttgagc tccttgggga
     7741 tcaggaagtc cttcgcgatc cggaccttct cgaattcgag gtagcccggc agccgcacga
     7801 tctccatgcg gtcgagcagc gcggagggga tggagtacag gctgttcgcc gtcgtgatga
     7861 acatgacctg cgagaggtcg aggtccacct cgaggtaatg gtcgttgaac gtgtggttct
     7921 gctcgggagc cagcacctcg aggagcgccg acgccggatc gccccggaaa tcggtgccca
     7981 gcttgtcgat ctcgtcgagg agcatgacgg gattgcgcga gccggccttg cgcagcgcct
     8041 gaacgatgcg tcctggcatg gacccgatgt aggtgcggcg atggccgcgg atctccgcct
     8101 cgtcacgcac gccgccgagc gacatgcgcg agaacttgcg ccccagcgcg cgcgcgatcg
     8161 acttcccgag ggaggtcttg cccacgcccg gagggccgac gaagcagagg atcgggccct
     8221 tcaacgaccc ggccagcttc atcaccgcca gatattcgag gatccgctcc ttcacctttt
     8281 tcaggccgta atggtcctcg ttcaggatct tctcgaccgc agcgatgtcc ccgttgtcgt
     8341 cggtgaggac gctccaggga agggcgacca gccagtcgat gtagttgcgg acgacggtgg
     8401 cctcgggcga catgaaggac atcttgccga gcttgtccaa ttccttgatg gccttttcgt
     8461 ggacgtcctt cggcatcttc gccttcttga cttgggcaac cagctcctcc acctcgttgg
     8521 agaactcgtt ggcgtagccc agctccttgc ggatcgcctt gagctgctcg ttgagataga
     8581 actccttctg gttcttcgtg acctggctcc ggacctggcc ctcgatcttc cgctcgagct
     8641 tcaagatctc gagctcgctc gcgagcgtct tggagagcag ccggaagcgc tcgaggacgt
     8701 cgtcctcctc gaggatcgcc tgcttcacgt tgaccttgag caggaggtgc gacgccacgg
     8761 tgtaggagag cagcaccggg ttcgtgatgt tgttcgcggt ggcgagcacc tcgtccggca
     8821 cgcgccggtt caggtgcacg tagtcgttga aggaggcgag gacgttccgc atcagagcct
     8881 cgagctgagc gtcctgcttc acgtcgtcct tgaggatgcc cacggccgcc gacatcgagt
     8941 ccttctccga gaagaacttc ttcccgcgcc cccggcagat gccttcgacc agcacgcgca
     9001 tggtcccgtc cggaagacgg aagagctgga gcacgcgcac gacggtcccg acgcggtaga
     9061 ggtccttgga ggccggatcg gccacctcgg ggcgcttctg ggcgagcacg aacacgacgc
     9121 ggtcgcgctg gacggccgct tcgatggcgt tcaccgacgc gttccgcccc accaggagcg
     9181 gaatggtcat gtagggatag atcaccacgt cgcgaagcgg gaggagcgga atcttgtccc
     9241 ggatctcgaa cacttcgccg tcacgctcga tacggatcat gaggttccct cggtgctgaa
     9301 aaccgccgcc aacccggggc acgggcccgg ggccagccgg ccggagtcgt gtttcggtac
     9361 ggttgctacg gtcttagatg ccgcgggggc cgcgccgctt cggccccggc gcggggggct
     9421 tcctgcttgc gagtcttacg cttccttgcg ccggcggtct ccgcggaagg cgaggatcgg
     9481 aggcgtcttc ttctcgatga cttcccgggt gatcgtgcac ccgacgactt ccaggcgcga
     9541 cgggatctcg tacatgaggt ccagcatcga ctcttcgagc acggcccgga ggccgcgggc
     9601 gcccgtgccg cgcttcagcg ccagctcggc gaccgcttcg aggccatcct ccgagaactc
     9661 cagcttgacc ccttccatct cgaagaactt ctggtactgc ttggtgatcg cgttcttcgg
     9721 cttggtcagg atgtccacca gggaggactt gctgagcgaa tccagcgcgc cgatgaccgg
     9781 gaggcggccc accagctcgg gaatgagccc gtagcggagc agatcctcgg gctcgaccag
     9841 ggaaagcatc tcgcccaggt tccggtcctt gcggctcttg atgtcggcgc cgaagcccat
     9901 cgccttgttg ctcacgcgcc gctcgatgat cttctccaag ccctcgaacg cgccgccgca
     9961 gatgaacagg atgtccttgg tgttcacctc gatgtacttc tgctgggggt gcttccggcc
    10021 gccctggggc ggcacgttgg ccaccgtgcc ctcgaggatc ttcaagagcg cctgctgcac
    10081 gccctcgccc gagacgtcgc gcgtgatcga ggggttttcg gacttgcgcg agatcttgtc
    10141 cagctcgtcg atgtagacga tgccgtgctc cgcgttcacc acgttgaagt cggtggcctg
    10201 gagcaggcgg accaggatgt tctccacgtc ttccccgacg tagcccgcct cggtgaggca
    10261 ggtcgcgtcg acgatggcga aggggacctt gaggatccgc gcgagcgtgc gcgccaggag
    10321 cgtcttcccg gtgccggtcg gaccgacgag gaggatgttc gacttctcca gctcgacgtc
    10381 ttcgacggcg ccgccggagt agatgcgctt gtagtggttg tagaccgcga ccgagagcgt
    10441 cttcttggcg cgatcctgcc ccacgacgta atcgtcgagg accttcttga tctccgccgg
    10501 cttcgggagc gagcccttgg gaagccccgc cgcatgatcg gtttcgcctt cgaggatgtc
    10561 gttgcacagc ttgacgcact cactgcagat gtagaccgac ggaccggaga cgaggcggct
    10621 gacctcgtcc tggccgcggc cgcagaagct gcagcggatc ggatgcggcg aatggacgcg
    10681 cttcttcatg ggctaggtct ctcgagcctg gtcagccgac gggcaccggc tcgggcttct
    10741 tctccttgag gtccattttc gtgatcacct tgtccacgat gcggtacgcc ttggcttcct
    10801 ccgccgacat gaagaagttg cggtcggtgt ccttctcgat cttgggaagc ggctgatcgg
    10861 tgtgccgcac gaggatcttg ttcagctcgg tgcggagcgt gaggatttcc ttggtataga
    10921 tctcgatgtc ggtcgcctgc ccctggctgc cgcccagagg ctggtggatc atgatgcgcg
    10981 agtgcggcag cgccgagcgc ttgccgttcg cgccggccgc gagcaggagc gccgccatgc
    11041 tggccgcctg ccccatgcag atcgtcgcga cgtccggctt gatgaactgc atcgtgtcgt
    11101 agatcgccag cccggccgac accacgccgc ccggcgagtt gatgtacagg ttcacgtcgc
    11161 ggtccgggtc ttccgattct aagaatagga gctgcgcgat gaccaggttc gccacgacgt
    11221 cgtcgatcgg cgtcccgatg aagatgatcc tgtccttcaa gaggcgcgag aagatgtcgt
    11281 aggcccgctc tccgcgcccg ctctgctcga ctacgatcgg caccagcgac atgtgcggct
    11341 tcctccctgg gacatctccg acgccttccg gcccgatgga ccaaacatcg ggaacaaggc
    11401 gcgtcatctt aaggcgttac gatcaatgtt ttggctcggg cccgctcccg gaggagcgcc
    11461 agggccttcc gttcggaaat gtgggcccgg acccggtccc gctgcccttc gtggcgcagg
    11521 aggcgctcgg tctcggcctc gttcatctct ccctcggcgg cggcgcggat cgcctgatcg
    11581 atctcctcgt cggagacctc cagcttctcc acccgcgcga gcgcgtcgag gagcaggtcc
    11641 cgcttgagcc gcttctcggc gtgcggccgg tattcacccc ggaaccgctc cgcctcttcg
    11701 tctgaaagct cccggccttc gcggtttaga cgctgcagga ggtcttccag atactcggcg
    11761 atcatcgatt ccggcagctc gaaggggttc ttctccacga gctgcgcggc cagcgcttcg
    11821 tcctgctccc gatggagcgc gagccgcgac tcaccctcca tattgagccg aatgcgcgcg
    11881 cgaagctcgg ccaaatcgcg catgcccgcc agctcgacgg cgagctggtc gttcagctcg
    11941 cggggacggc gctcgcgtac cgccttcacc tggacggcga aggtcacggt gcgcccgcgc
    12001 agctcctcgt tgccgaactc ctggggatac tgcacgtcga acgtgacgtg gtcgcccgcc
    12061 ttccggccga ccaggtgctc ccggaactcc ggcaggaggc ccgaggcgga gagctcgatc
    12121 tgcgcgtcct tctgttccga gcgccggacc ggctttccct tggcgttcag ccgggcgtag
    12181 tccaccagga gggcgtcccc ctcgctcgcc gcccgatcga cgtcctccca ctgcgcggcg
    12241 cgctgccgga gatcctcgag ggctttctcg acggcctcgt cggggacttc ggtgtcgcgg
    12301 gtcggcagct ccaaaccctc gtagttctta ggggttacgt cgggccgaac gtccaccttg
    12361 gcctcgtagc tgagcggctt cccgcgatcg aaggtgatct ttccgatctc cgggaaggtg
    12421 atcggctcga gcttggattc gcgcacggcg gactcgtagg agcgcttgac cgcccgctcg
    12481 agcacgtcct gctcgaggga atggccgaac tgcagctcca ggacgctccg gggtactttc
    12541 ccctttcgga accccggaac ggcggccttg cggcggtaat cctcgatcac cgcctcgaaa
    12601 tcggcgtcca gctcctccgc cggtacctcg atggatagcg tcttctgcca caccccggtg
    12661 tctcgaacgt cgaccctcaa accgtcactc ctggttaagt ggtgcgagag gggggagttg
    12721 aacccccacg agttgcctca cgggatccta agtcccgcgc gtctgccagt tccgccactc
    12781 tcgcaagcgg actgtgcctt taggtgccct agatggtagc accgaagtga ttgggcaaca
    12841 agcagattgg gcttggatcg gccgctctca ggcgcgggcg cccgggacca ccgcttcgac
    12901 cacccgggcg accacctcgt agcggccgaa ggagggcatg agataggccc cttcgaccag
    12961 cgcgcgggct tcgaccagga gatcgaggca catttcgatg ccgacatcga tcccccggtc
    13021 cccggcttcc cgcatggcct tgcgggcagg gtcgggaatc acgatcccgg gcacctcgtt
    13081 gtggaggaat tcggcgtggc ggtggctctg gagcggcaat agcccgagaa ggaccgggat
    13141 ccgcggcttt tttgcagcct cgaggaagcg cgtcagggtg gcaagctcgt acaggggctg
    13201 ggtcatggcg aattcggcgc ccgcctccac cttggcgtgg aagcgctcca gctcctggtc
    13261 gaagttctcg gccgaggggt tcacggcgca gccgatcacg aagcgtgtcg gctccccgat
    13321 cgagttgccc cccaggtcca cccccgcgtt cagctgtccg agcacccgga tcaggccgat
    13381 cgagtccacg tcgaacaccg cggtggtgtt cgggtagtcc cccgcgcggg gcgggtcgcc
    13441 cgtcagggcg aggatgttcc ggatgccgag cgcgtgggcg cccatgagat ccgactggat
    13501 ccccatgagg ttccggtcgc ggcaggtgaa gtggaggatc gtctcgatcc cgacctggct
    13561 ctggatcatc gtcgcgagcg cgatcgagga catgcgcacg cgggccatcg ggctgtccgc
    13621 cacgttgatc atgtccacgc cggacgtctt caagagcgtg gccccctcga tcatcttccg
    13681 tggccggatg ccgcggggcg ggtccacctc gacgctcacg acgaactccc gccgcgcgat
    13741 cttggcggca aggcgcgagc gctgcgcggg atcgatggag ggcgccgttt cctcgacggt
    13801 ggcgcggggg cgatcgcggt cccggtcccg ctcgacggtg acggtgccgc gcggagcgtg
    13861 ccgctcgcgg gaggaatcgt ccgctccggc gtcgcgccct tctgagggca ccgccgccgt
    13921 gccggtcccg ctcgggctct cccgccttcg cgcgagagcg tcgtgcatcc gccgcacgtg
    13981 cgccggggtg gtgccgcagc agccgccgat cagccgcgcg cccgcctcgg cgaaacgcac
    14041 cgcgtagtcg gcgaagtact cgggcgagga gaggtagagg aagcggccgt tcaccaggcg
    14101 cggcaatccg gcgttcggca tcaccgcgat gggacgggtg cccttccgcg cgtagcgatc
    14161 cacgatctcg agcagctcgt ggggtcccac gctgcagttc gcgccgatca cgtccaccgc
    14221 cttggcggag agcgcgcgca ccgcgtcctc gggataggtg ccgtgcaggg tgcggccgtc
    14281 ctcggtgtag gtcatctcca ccaccagcgg cagatcggag gtgaccgatc gcacggcatc
    14341 gacggcgagg agcgcctcgg agaggtcggt ctgggtctcc acgatcagga gatcgacgcc
    14401 tccctcgacc agcccctccg cctgctcacg gtaggcggcg agggcctcgt cgcgcgggat
    14461 cgtcccgagc ggcgccaccg gctttcccag cgggccgatc gcccccgcca cgaaaaccgg
    14521 ctcccccacc tcttcgcgcg cgttccgcgc gatctgcgcg ccggcgcggt tcacctggcg
    14581 cggcgagtcg gtcacgccgt gcgcggcgag gcggaatcgg ttcgcgccga acgtgttcgt
    14641 ctcgatcacc tcggcacccg cgcggatgta ctcgcgatgg accgactgga tcagggcggg
    14701 ctcgacgagg ttcagcgcgt cgaaggagcg gtcgaagggg atccccttgt cgtagagaaa
    14761 cgtccccatt gccccgtcgg cgagcaggat tccctcggag aggcgcgcca ggaacggatg
    14821 gcgggcgcgg gcgccgggct cttccaccat gcgaggcatc gtactatact gcgcggctca
    14881 tcgctggaag ccaattgggg ggaccgtttt gaatcttctc gtcttcgtca agcaagtacc
    14941 cgacacggag acccgcattc aaatccagaa tggccaggtc gacgtctcga gcgtgaagtg
    15001 gatcgtgaac ccctacgacg aattcgcgat cgaggaggcg ctgcggatca aggagcgcct
    15061 gggagcgggc agggtcaccg tcgcgagcct ggggcccgag cgggtgaagg aagcgatcaa
    15121 gtacgggttg tcgctgggcg cggacgaagg ggtctggctc aagggcgacg gcgtcgccct
    15181 gggcgatccg ggctcggtcg cgaccgtgct cgcggccgcc gcgaagaaga tcggcttcga
    15241 cctgatcctc accggaaagc agggcgtgga ccaggactgg tcgcaggtgg gcgtgatgct
    15301 ggccgagcgc ctcgacgtgc cgcacgtctc ggtggtcgtg acgttgaacg tcgacgcggg
    15361 cgccaaaaaa ggcaccgcgc atcgtgaagt ggaagggggc gccgagacgg tcgagttcga
    15421 gctgcccgcc gtgatcacgg cccagaaggg gctgaacgag ccgcggtacg cctcgctcaa
    15481 gggaatcatg gccgtgaaga agaaggtcat tcccgaatgg acgctcggcg atctcggcgt
    15541 cgatgccgcg tcggtgggag acgccgccgc gggcgtccgc attctcgaag ccctgccgcc
    15601 gcccgcgcgc tccgcgggac gcgtgctcga gggcgagccg gccgattccg cgcgggagct
    15661 ggtgcgtctc ctccgcgaag aagcgaaggc gatctgatgg ccaacccggt gaaagtcgcg
    15721 gtcgtcgcgg agacgaaggg gggcgcggtc cggaagatcg cgctggaaat gctggccgag
    15781 gcgcggcggc tggccggcga ggcgggcggc acggtcggcg cggtagtgat cggaccctgt
    15841 tccgccggcg aggcggagcg cctcgcgcag catggcgcag atcgtgtcgt gcacgtcacg
    15901 ggcgccgcac tggagaccta
//
LOCUS       DASSLZ010000353         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1877406, whole genome shotgun sequence.
ACCESSION   DASSLZ010000353 DASSLZ010000000
VERSION     DASSLZ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877406"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFM00_13420"
     CDS             <1..607
                     /locus_tag="VFM00_13420"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HET7499291.1"
                     /translation="GVDRLRAVLDLAGGFTPEADSTATRVERPDDAPRDSAFQSLAAR
                     NPDFLTPADRDFVVSSARERRAVSANVGAFLARGDPRGDVTLHDGDRIVVPRRVALVS
                     VQGEVRAPGYVPYVSGRDAGDYVKDAGGFTSRARKGHIRVTRAATGGMVSLSETGELR
                     PGDAIWVPAKPERSTWASVRDVLTTAAQVATIYLVVREATK"
     gene            604..1680
                     /locus_tag="VFM00_13425"
     CDS             604..1680
                     /locus_tag="VFM00_13425"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HET7499292.1"
                     /translation="MKAIEPAFRESPNPSMGDLMGVLRRRRRFLALNVAATTLIALVI
                     ALLLPKWYTGRAVLLPPTEDDLGSSVVSQLMPRGLGSLRMPGAPTLADVFVAVLKSRT
                     VADRIVRRFDLVHRYDVPDAEKAVKELEGHVTFRVGDEGTIAILAEDKDPKTAAAMAN
                     AYIEELDRFNKETRTTSATRTRAFIEQRLEVAKRDLAAAEDLLRDYQQRKRLPSLSPT
                     DRSDAEMGAQLMAQRTALEVKLQVLRQSLAENSEEVRRVREELAAVDRQVGTLPAAGL
                     DVARLYRDVKVQEQVFELLTSQLEEARIRETRDTPTVQVLDRAEPPLHKSRPRRSVIV
                     IAGLLIGLLGSAAAALLLERRAAG"
     gene            1682..2455
                     /locus_tag="VFM00_13430"
     CDS             1682..2455
                     /locus_tag="VFM00_13430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET7499293.1"
                     /translation="MRRLIRGTARLLNRARGYHPARIGGERFRTDPDHIGFWRDVSRG
                     RWEPATFRFLEQSLRPDSVFADVGAWIGPTTLFAARRCRLVYGFEPDHDAYRYLLWNL
                     HLNSLPNVVPMNAALGAETGMRPLYVAGGRQGTSQSSLLAAAGQPSMPVFSIAWRDWL
                     EIAKPGRIDCIKMDIEGGEFELLPAMRAQLEAERPALHLSLHAGRLPERERKERLDGV
                     LDLLRVYPSWRNEAGEPVTPEAFARAALDGDCVLLLTNG"
     gene            2603..>2868
                     /locus_tag="VFM00_13435"
     CDS             2603..>2868
                     /locus_tag="VFM00_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499294.1"
                     /translation="MPRILGIVVASLLTQAPALALAWPSPHTLGGYAYVSPAPGSALH
                     HPETNVIVRPGGTVDPATLASSTIQASGSVSGAHPGFLRLSDDG"
BASE COUNT          391 a         1060 c          999 g          418 t
ORIGIN      
        1 cggcgtggac cgcctccgcg cggtcctcga ccttgccggc ggattcacgc ccgaggccga
       61 cagcacggcc acgcgcgtgg agcggcccga cgacgctccg cgcgactccg cctttcagtc
      121 gctcgcggcg cggaatcccg acttcctcac gccggccgac cgcgacttcg tggtcagcag
      181 cgcgcgcgag cggcgtgccg tgtcggcgaa cgtgggggcg ttcctcgccc ggggcgatcc
      241 gcgcggcgac gtcaccctgc acgacggcga ccggatcgtg gtcccgcgcc gcgtggcgct
      301 ggtctcggtg caaggggagg tgcgcgcgcc gggctacgtg ccgtacgtgt ccggccggga
      361 cgccggcgac tacgtgaagg acgccggcgg cttcacctcc cgggcgcgga aggggcacat
      421 ccgcgtgacg cgcgcggcga ccgggggcat ggtgagcctc tccgagacgg gggagctccg
      481 ccccggcgac gccatctggg tcccggccaa gccggagcgc agcacgtggg ccagcgtccg
      541 cgacgtgctg accacggcgg cgcaggtggc cacgatctac ctggtcgttc gcgaggccac
      601 gaagtgaaag cgatcgagcc cgcgttccgc gagtcgccga acccctcgat gggggacctg
      661 atgggcgtcc tccgccgccg gcgccgcttt ctcgcgctga acgtggcggc aacgacgctg
      721 atcgcgctgg tgatcgcgct gctccttccc aagtggtaca ccgggcgcgc cgtcctcctg
      781 ccgcctaccg aggacgacct cgggtcgagc gtcgtctcgc agctcatgcc gcgcggtctc
      841 ggaagcctgc gcatgccggg cgcccccacg ctggccgacg tcttcgtcgc ggtgctgaag
      901 agccgcaccg tcgccgaccg gatcgtgcgc cgcttcgacc tcgtccatcg ctacgacgtt
      961 cccgacgccg agaaagcggt gaaggagctg gaggggcacg tcacgttccg ggtcggggac
     1021 gaggggacga tcgcgatcct ggccgaggac aaggacccga agacggcggc cgccatggcc
     1081 aacgcctaca tcgaggagct ggaccgcttc aacaaggaga cgcgcaccac cagcgccacg
     1141 cggacccgcg ccttcatcga gcagcggttg gaggtggcca agcgggacct cgcggcggcc
     1201 gaggacctgc ttcgcgacta tcagcagagg aagaggctcc cttcgctctc tccgaccgat
     1261 cggagcgacg ccgagatggg ggcgcagctc atggcgcagc ggaccgcgct ggaggtgaag
     1321 cttcaagtcc tgcgccagtc tctggccgag aacagcgaag aggtcaggcg cgtgcgcgag
     1381 gagctggccg ccgtcgaccg ccaggtcggg acccttcccg ccgcgggcct cgacgtcgcg
     1441 cgcctctacc gcgacgtgaa ggtgcaggag caagtcttcg agctcttgac ctcgcagctg
     1501 gaggaggcgc gcatccgcga gacgcgcgac acccccaccg tgcaggtgct ggaccgcgcc
     1561 gagccgccgc tccacaagtc gcgccccagg cggtcggtca tcgtgatcgc cgggctcctg
     1621 atcggtctct tgggaagcgc cgcggcggcg ctcctcctgg aacggcgcgc cgcggggtga
     1681 cgtgcggcgg ctgatccgcg ggaccgcgcg gctcctgaac cgggcgcgcg ggtatcaccc
     1741 ggcgcggatc ggcggcgagc gcttccgcac cgatccggac cacatcggct tctggcgcga
     1801 cgtgagccgc ggccgctggg agccggccac gttccgcttc ctcgagcagt ccctgcgtcc
     1861 cgattccgtg ttcgccgacg tcggcgcgtg gatcgggccg acgacgctct tcgcggcgcg
     1921 ccgctgccgc ctcgtgtacg gcttcgagcc cgaccatgac gcctaccgct atctcctctg
     1981 gaaccttcac ctgaactccc tgcccaacgt cgttcccatg aacgcggccc tcggcgcgga
     2041 gaccggcatg cggcccctgt acgtcgccgg tgggcgacag ggaacgagcc agtcctcgct
     2101 gctcgccgcc gcgggccagc cctcgatgcc tgtgttctcg atcgcgtggc gggactggct
     2161 ggagatcgcc aagcccggcc ggatcgactg catcaagatg gacatcgagg ggggcgagtt
     2221 cgagctgctc ccggcgatgc gcgcccagct cgaggcggag cgtcccgcgc tccatctgtc
     2281 gctgcacgcc ggacgccttc cggagcgcga gcggaaggag cgcctcgacg gcgtgctcga
     2341 ccttctgcgc gtctatccct cgtggcggaa cgaagcgggg gagccggtca cgccggaggc
     2401 gttcgctcgg gccgcgctcg acggcgactg cgttctgctg ctgacgaacg gttaggcgcc
     2461 ctcggtgtac cgatcctcgg cggccggctg ctccccaagt ccaccttgtc atcaggcgcg
     2521 cgccatgttc gaatgacgtc ctcccccaat ggcgtggtct tgcacgggcc gcattcccgt
     2581 ctcttcggcg caggtgaacg gaatgccacg gattcttggg atcgtcgtcg cctccctgct
     2641 gacgcaggcg cccgcgctgg ccttggcgtg gccctcgccc cacactctcg gcggctacgc
     2701 gtacgtctcc ccagcgcccg gctccgcgct ccatcacccc gagaccaacg tcatcgtccg
     2761 ccccgggggg accgtcgacc cggccacgct cgcgagctcc acgatccagg cctccggatc
     2821 ggtgtccggc gcccaccccg gattcctccg gctctccgac gacggaca
//
LOCUS       DASSLZ010000354        12618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_748187, whole genome shotgun sequence.
ACCESSION   DASSLZ010000354 DASSLZ010000000
VERSION     DASSLZ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12618
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748187"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1487)
                     /locus_tag="VFM00_13440"
     CDS             complement(12..1487)
                     /locus_tag="VFM00_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7499295.1"
                     /translation="MLRIRSRALAALLVSGLVAAGAVPALAGICFTSGKVYVQQKVWD
                     KAASQLECARKQEPQNLQVYSLLGIARAELGQYAASGAAFQMGIQAATAKKDEKKAAD
                     LRNNQMAYMSRLYNSGVKAMSGAGTVAETEEQEGAKRLFIAPAPPEVAITDTTVFGKY
                     EGDSKVEEAAYYFRLATLMDPTAVDAYKNLSYVYEIMGHTDDAMDAAKAGLKLAPNDE
                     KLSRNLRAAAVGRANRLFKAGKWSEAIVAYHKAIEDDPASKLTYEARIAEANFNWSRE
                     LDEKDPNRKAALDSTVVAYRELLRDAPKDSATASMRENAYYNTSVILANQTRYADAAK
                     SLDEATAEFPNSKDLLSLSGQTKFQAGDFNGAVSDLKRALAIDPKDASVHQFLFYSLN
                     KLNKQSESVAEYSIYKALSEGKARTGSDLKKWVDSADMRLGPKHQLKKTVTSDGYPEE
                     VRTFQDGDKALESWFYWSKGKVITFMDGQVLSQATFPPSKS"
     gene            complement(1666..3666)
                     /gene="priA"
                     /locus_tag="VFM00_13445"
     CDS             complement(1666..3666)
                     /gene="priA"
                     /locus_tag="VFM00_13445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET7499296.1"
                     /translation="MRVAVPIPKNDTYVYALPDGEPPEPGLRVLVPLGRRRLWGTVLE
                     EVAEAPPGVAVRPLLGIPEPRLILTPEILALCRWVADYYAAGLGETLAAAAPALTALR
                     RRAPRAPDDADDAAHGGPPPSRDALNQEQRAALEALEASVAAKEFRAFLLYGVTGSGK
                     TAVYLHAALAAIRAGGQALALVPEIALAPQAADAFRRAGVRVALYHSDLRPRERVDVW
                     RSAAAGELDVVVGTRSAVFLPMPRLRLIVVDEEQDGAYKQDEAPRYHARDVALVRAQR
                     LPITAVLASATPSLESYARASRGDCTLLRLPHRVDGRPLATVRLADLRHRVRSLDGPS
                     SKHLTPPLLEAMARTLERKEQGILFLNRRGHSTYLQCRGCGQVARCEHCDVPLTVHAE
                     DSILRCHYCNASRRLASACPSCGAADLWFGGVGIQKIEREVARLFPEARIARLDLDAV
                     RRRGSAAAILRAFRARQVDFLLGTQMVTKGFDFPGVTLVGVIVADLQLYLPDFRAAER
                     TFQLLTQVAGRAGRGDQPGEVIMQSYDPEHPALRAAAAQDFEIFYAREARERRELSYP
                     PFGHLVEVEVSGKGAERVYKGGMRVKQLFARAKGADALEILGPAPKPLARLVGRERWH
                     VLVRGNSRPALRSVLEEVLPKLRTERLPGLRLAVDVDPHQLL"
     gene            complement(3755..4156)
                     /locus_tag="VFM00_13450"
     CDS             complement(3755..4156)
                     /locus_tag="VFM00_13450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7499297.1"
                     /translation="MSKGKILVVDDEIYIVHILDFSLGMEGYEVVTALDGEQALERVA
                     SEKPDLIVLDIMMPKLDGYEVCRAVKSNPATKNVPVILLSAKGRNVDQKMGFDVGADD
                     YITKPFSPRKLVERINQLLGQAISERPASSA"
     gene            complement(4202..6778)
                     /locus_tag="VFM00_13455"
     CDS             complement(4202..6778)
                     /locus_tag="VFM00_13455"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7499298.1"
                     /translation="MRSVELLWALAATALLLFEALGRRRSLRRELKEARASYESALAR
                     NEQLTRAADDMATLYRNELLSSRKRAARLKKVLEIATSINSNLTLDKVLHEIVHAVAD
                     AAGFKIVLLRVLNPRGDCFEARAFAGLDREAVMKLEQKPVPRAQFESWLQDKFRIGRS
                     FFIGHEAKFWGERDEDGFTPDLGERKAGEWNAQDVLFVPLRTKDGTVIGYLSVDDPVD
                     RRRPSKEIVETLEILATHAVVALQNSELYERLHESMRQLEEATERAEELNDLKSSFVS
                     IVSHELRTPLTHILSYAEALLENVGTPNVGMQREFLGVIHEQSQKLKRLIESILELSQ
                     LESGRFRMHREPFNLVEVAEESAGLLHEMALEDGVDVDVEAPSREVVVEADRSLVRRI
                     LLNLGQNAIKFTERGGKVRIRVFGEGRVARIRVEDTGIGIPREELDKIFDKFYQIDGS
                     LSREHPGVGLGLSIARSIVEWHGGEIHAESEPGKGSSFTVTLPATPGDAGVITHAGWD
                     SGRTASDHLTRLTVEMIAEVMHARIASLMLVDEEREELYIKAARGLREEVVAGTRLKM
                     GDSIAGWVAKHGVPLLVTDVEEDPRFTRRNSHQYETKSCLSVPIKAEGRVVGVININN
                     KISCTPFTEDDQALLTSLADRAARAWEHAEAVDSSSDRAEEAARAMAAIIDNARRARL
                     KLTAGSMATRAAAIGRRLGLSEDDLRVLAYVASIHDIGMAQIPSPVLHEPGQLDAETW
                     AQVEKHPARTVEIVRPIEFESRAAELIMAHHERLDGRGYPRGLKGEQIPIGARIIAVL
                     DAYESMTLGRPYRQAMSHDDAIAELRRCAGSQFDPKVVEAFVQTAGAQEDTKAFHAPE
                     AA"
     gene            complement(6765..9149)
                     /locus_tag="VFM00_13460"
     CDS             complement(6765..9149)
                     /locus_tag="VFM00_13460"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7499299.1"
                     /translation="MISEGGQGRGRLLGSLASLAFAAQSAFDTRGLVNALAQHVLEIA
                     RADGFALFLLDYETGEMKGDAFERGERAPVGHARYAPRSNAFLERVLRREALVVETPP
                     PATASENAIPWRDGSFESVIGVPIVVGTALLGVAVLGFRRSVTLTDRRRRALRYLADQ
                     IGLALDRLRARAEIETKTRQLEEARAALQRIDEAKSDLISIVSHELRTPLTAIKAYTE
                     TLLDNADAASFPLHKRFLGIIDEECDRLSRMVNDVLDLSRMDSGRRRLNTVLVSLAKL
                     IEEVAPTVEPGLRQRGLRLRLSLEPALPPIEADPDLLKQVLVNLIHNAVKFSREGSAI
                     TVSAFGQGDRIRIGVEDQGPGIAKDQIARVFDRFYRVEPEGGERVAGTGLGLAIVKRV
                     VQLHGGAVTVESEVGHGSRFLVDLPREQQGFRALIQSLTPLFEQPELRNLLQVSVEMI
                     AEVMDAGIVSVLFFSEDGSEMAIHAAHGLDADTVARTRVRAGDSIAGWVARTSENLLV
                     ADIETDRRFRKMNHPQYETKSLLCVPLRLAGETVGVVNVNNKRNGLEFDADDLGLLAA
                     VVKRVAAAVDRVRGDVSPETLEATLRTIRAILRARRTHLLPGSRRAFKLAVELGRRVG
                     LSAEDAEVLGYVARVHDVGMLEIDDGLLLSARRWSPEERRDVEGHPQAGVQMLRPIEL
                     TSKVNEIILGHHEHWDGRGYPRGLAGDQIPLAARVLAVVDAWESMTAGRPYREPVGEA
                     EAIAEIRRCGGTQFDPRVVEEFARLLAEEAAPDAKGRASEARREPASWRPDALG"
     gene            complement(9146..10174)
                     /locus_tag="VFM00_13465"
     CDS             complement(9146..10174)
                     /locus_tag="VFM00_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7499300.1"
                     /translation="MTEPSPAESGRILVIEDEPRLRFILERQLREAKDARYEVRTAED
                     GVKGLAEVMREPPELILLDVMMPGMDGFEVCRRLKSNPLTSRIPIIFLTAKSTVDDRL
                     RGLELYADDYLTKPWEQQELLFRVRNQLKTRRAQLSSNALTGLPGNVLIETELNRRIG
                     AGEKFAFLHLDLDAFKAFNDYYGYARGDALIRFTAALLQEQVRQHGGEGDFVGHIGGD
                     DFVIITVPARARAIAEAVKADFDARVPEQYDPLDRERRQITVLSSRQGGWRDFDILSI
                     TILGVMNVGVEIQHSAQVSDRAKDLKKVGKAVKGSVIVFDRRSDGSPAVPAAPDPPAD
                     GSRGGGAP"
     gene            complement(10362..10754)
                     /gene="mscL"
                     /locus_tag="VFM00_13470"
     CDS             complement(10362..10754)
                     /gene="mscL"
                     /locus_tag="VFM00_13470"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET7499301.1"
                     /translation="MLTEFRKFLLQTNALALAIGVIIGAAVGKVVSSLVGDILMPVIG
                     LMIPGGAWREQKWVLSVKPDGTPANAITYGAFLGNLIDFVIIAFVVFMITKALLKPEA
                     GPPTKECPECKEIIAASARKCRACASPV"
     gene            complement(10823..11824)
                     /gene="tsaD"
                     /locus_tag="VFM00_13475"
     CDS             complement(10823..11824)
                     /gene="tsaD"
                     /locus_tag="VFM00_13475"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET7499302.1"
                     /translation="MRVLGIETSCDDTGAGIVEDGRLLGHVVRAQDVHAEYGGVVPEL
                     ASRAHITLLPKILDELLAKTGLSLADIHGVAATAGPGLVGSLLVGLEFAKGLSMARGI
                     PLAAVNHVEAHLLSPALENDLPFPYLGMVASGGHTEIYHVPERGRAVRLGSTRDDAAG
                     EAFDKVGKLLGLPYPGGPEVDRLAREGNPDAIAFPVARLGADTLDVSMSGLKTAVKLL
                     VEKEPDPIPPARERDIAASAERAIVTALIERLSLALDRYPARALAIAGGCACNTRLRR
                     DAATLAASRGIPALFPSPPLCRDNGAMIAYAGWAALTAGRVSPVTVSAVPNLDSFPA"
     gene            complement(11865..12266)
                     /locus_tag="VFM00_13480"
     CDS             complement(11865..12266)
                     /locus_tag="VFM00_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499303.1"
                     /translation="MKAASRSASRHRTPAAASVFLLLALLSEVGCAHWQPVQRERIAD
                     TLRDSKPDYARVGAGGSVLLVQHPEVRADTLHGTMRADHRAPVTIPLASVDSLSLYRN
                     GRTDARLFAIGMLTISVLFVAAALNVNRSLQ"
BASE COUNT         1878 a         4301 c         4428 g         2011 t
ORIGIN      
        1 cggtgcgtcg ttcaggattt ggaaggcggg aacgtcgcct gggaaagcac ctgtccgtcc
       61 atgaaggtga tcacctttcc cttgctccag tagaaccagc tctccagggc cttgtcccca
      121 tcctggaacg tgcgcacctc ctcgggatac ccgtccgagg tgaccgtctt cttgagctgg
      181 tgcttggggc caagacgcat atcggcagaa tcgacccatt tcttgaggtc gcttccggtc
      241 cgcgccttgc cctcgctaag cgccttgtag atcgagtatt cggcgaccga ttcggactgc
      301 ttgttcagct tgttcaagga gtagaagagg aattggtgca cggaggcgtc cttgggatcg
      361 atggcgagag cacgcttcag atcgctgacg gcgccgttga aatcgccggc ctggaacttg
      421 gtctggccgg acagcgagag gagatccttg ctgttcggga actcggccgt cgcctcgtcc
      481 agcgacttgg ccgcgtcggc gtaccgcgtc tggttcgcga ggatcaccga agtgttgtag
      541 taggcgttct cgcgcatcga ggccgtggcg gaatccttcg gcgcgtcgcg gagcagctcc
      601 cgatacgcga ccaccgtgct gtccagggcc gccttgcgat tcgggtcctt ctcgtccagc
      661 tctctcgacc agttgaaatt ggcttcggcg atccgcgctt cgtacgtcag cttgctggcc
      721 gggtcgtcct cgatggcttt gtggtaggcc acgatcgcct cgctccactt gcccgccttg
      781 aagagccggt tggcgcgtcc caccgccgcg gcgcgcaggt tgcggctgag cttctcgtcg
      841 ttgggcgcga gcttcagtcc cgccttggcc gcatccatgg cgtcgtcggt gtggcccatg
      901 atctcgtaca cgtaggagag gttcttgtac gcgtccaccg cggtcggatc catgagcgtg
      961 gcgaggcgga aatagtaggc ggcctcctcg accttggaat cgccctcgta cttgccgaag
     1021 acggtcgtgt cggtgatggc gacctcgggc ggcgccggcg cgatgaagag ccgcttcgcg
     1081 ccctcctgct cttcggtttc cgccaccgtg ccggcgcccg acatggcctt caccccgctg
     1141 ttgtagaggc gggacatgta ggccatctgg ttgtttcgca ggtccgcggc cttcttctcg
     1201 tccttcttcg cggtcgccgc ctggattccc atctggaagg cggcgcctga ggcggcgtac
     1261 tgcccgagct cggccctcgc gatgccaagg agggagtaga cctggaggtt ctgcggctcc
     1321 tgcttccggg cgcactcgag ctgcgatgcc gccttgtccc acaccttctg ctgtacgtac
     1381 accttgccgc tggtgaagca gatgccggcg agcgcgggga cagccccggc ggcaaccagg
     1441 ccggagacga ggagcgcggc aagcgcgcgc gagcggattc gaagcatcga ttcctccacg
     1501 atagaagacg gtcttcccgg gggccgtacg atcgttaata cccggagatg gggtgtcccg
     1561 ttccggcggt atcacgattg aatcacagcg gcgcgccgtg tcaagccccg ttcaggccgc
     1621 cgccaggcgt cggagccagc ccacaaccaa aagcgaatga atcgcttaca gcagttgatg
     1681 ggggtccacg tccacggcca gccggagccc tggaagccgc tcggtcctga gcttcggaag
     1741 aacctcctcc aggaccgacc ggagcgccgg ccgggagttg ccgcggacca ggacgtgcca
     1801 ccgctcccgt cccaccaggc gcgcgagcgg cttgggggcc ggtcccagga tctccagcgc
     1861 gtcggccccc ttcgcgcgcg cgaagagctg cttcacgcgc atccctcctt tatagacgcg
     1921 ctccgccccc ttgcccgaga cctcgacttc gacgaggtgg ccgaagggag ggtaggagag
     1981 ctcgcgtcgc tcgcgcgcct cgcgcgcata gaagatctcg aagtcctgcg ccgcggccgc
     2041 gcgcagcgcg ggatgctcgg ggtcgtacga ctgcatgatc acctcgccgg gctgatcgcc
     2101 gcgccccgcg cgcccggcga cctgggtcag gagctggaac gtacgctccg ccgcgcggaa
     2161 atcggggagg tagagctgca ggtcggcgac gatgaccccc acgagcgtca cgccggggaa
     2221 atcgaatccc ttggtcacca tctgcgtgcc caggaggaaa tcgacctgcc gcgcgcggaa
     2281 tgcgcgcagg atcgccgcgg ccgacccgcg ccggcgcacc gcgtccaggt cgagacgcgc
     2341 gatccgcgct tccgggaaga gccgcgccac ctcgcgctcg atcttctgaa tcccgacgcc
     2401 tccgaaccag aggtcggcgg cgccgcagga cgggcaggcg gaggccagcc ggcgcgacgc
     2461 gttgcagtag tggcagcgca ggatcgagtc ctccgcgtgc accgtcagcg gcacgtcgca
     2521 gtgctcgcac cgggccacct ggccgcagcc gcgacactgg agataggtcg agtggccgcg
     2581 ccggttcagg aaaaggattc cctgctcctt ccgctccagg gttcgcgcca tcgcttccag
     2641 gaggggcggc gtgaggtgct tggaggaagg cccgtcgagg gagcggacgc gatgccggag
     2701 atcggccagg cgcaccgtcg cgagcggccg cccgtcgacc cggtgcggca ggcggagcag
     2761 cgtgcagtcg ccgcgcgagg cgcgcgcgta gctctcgagg ctgggggtcg cggaggcgag
     2821 caccgcggtg atcggaagcc gctgcgcccg caccagcgcc acgtcgcgcg cgtggtagcg
     2881 cggcgcctcg tcctgcttgt aggccccgtc ctgttcctcg tccacgacga tcaggcggag
     2941 gcgcggcatc gggaggaaca ccgccgagcg cgttcccacc accacgtcca gctcgcccgc
     3001 ggccgcgctc cgccagacgt ccacgcgctc gcgcgggcgg aggtcgctgt ggtagagcgc
     3061 cacgcgaacg cccgcgcgcc ggaaggcgtc ggccgcctgc ggcgcgagcg cgatctcggg
     3121 cacgagcgcg agggcctgcc ccccggcgcg aatcgcggcg agggcggcgt ggagatagac
     3181 cgcggtcttg ccgcttcccg tgacgccgta gaggaggaac gcgcggaatt ccttcgcggc
     3241 gaccgaggcc tccagggcct cgagcgccgc gcgctgctcc tggttcagcg cgtcccgcga
     3301 tggaggcggt ccgccgtgcg ccgcgtcgtc ggcgtcgtcc ggcgcgcgcg gcgcgcggcg
     3361 ccggagcgcg gtgagggcgg gggccgcggc cgcgagcgtc tcgcccaggc cggcggcgta
     3421 gtagtcggcc acccagcggc agagcgcgag gatctccggc gtgaggatga ggcgcggttc
     3481 gggaatgccc aggagggggc ggacggcgac gccgggcggc gcttcggcca cctcttcgag
     3541 gaccgtcccc cacaggcgcc ggcggccgag cggaaccaga acgcgcagcc ccggctccgg
     3601 cggctcgccg tcgggaaggg cgtagacgta ggtgtcgttc ttgggaatgg ggacggcgac
     3661 ccgcacgaga cgcatcgcgg gcggatggta ccactcgcgc ggcggctcgc gggcacggcg
     3721 gatggggccg ccggcgcgcg aagcgcggcc gggactacgc gctcgaggcg gggcgctccg
     3781 agatcgcctg gcccaggagc tggttgatgc gctcgaccag cttccgcggg ctgaacggtt
     3841 tcgtgatgta gtcgtcggcg ccgacgtcga agcccatctt ctggtcgacg ttccggccct
     3901 tcgcggagag gaggatgacg ggcacgttct tggtggccgg attcgatttc accgcgcggc
     3961 agacttcata gccgtccagc ttcggcatca tgatgtcgag cacgatgaga tcgggcttct
     4021 cggaagcgac gcgctccagc gcctgctccc cgtcgagcgc cgtgaccact tcgtagcctt
     4081 ccatcccgag actgaagtcc aggatgtgga cgatgtagat ctcgtcgtcg accaccagga
     4141 tcttgccttt cgacatccga ctcacctcct cccagaccgt tctagggtgt ctatcggcgg
     4201 ctcatgcggc ttctggagcg tgaaacgctt tcgtatcctc ctgggcgcct gccgtttgca
     4261 cgaatgcctc cacgaccttg ggatcgaact gcgatcccgc gcagcgccgc agctccgcga
     4321 tggcgtcgtc gtgggacatg gcctggcggt agggacgtcc cagggtcatc gattcgtagg
     4381 cgtcgagcac cgcgatgatc cgcgccccga tcggaatctg ttcccccttg agtccgcgcg
     4441 ggtagccgcg cccatccagg cgctcgtggt gcgccatgat cagctcggcc gcccggctct
     4501 cgaactcgat cggccgcacg atctccaccg tgcgcgcggg gtgtttctcc acctgcgccc
     4561 atgtctcggc gtccagctgc cccggctcgt ggagtaccgg cgaggggatc tgcgccatgc
     4621 cgatgtcgtg gatgctcgcc acgtaggcca gcacccgcag gtcgtcctcg gagagtccca
     4681 ggcggcgccc gatcgccgcg gcgcgcgtcg ccatggagcc cgccgtcagc ttgagccgcg
     4741 cgcgccgggc gttgtcgatg atggccgcca tggcacgggc cgcctcctcg gcgcggtccg
     4801 acgagctgtc caccgcctcg gcatgctccc aggcccgtgc cgcgcggtcg gccagcgacg
     4861 tgagcagcgc ctggtcgtcc tcggtgaacg gcgtgcagga gatcttgttg ttgatgttga
     4921 tcacgcccac gacgcgccct tcggccttga tcggcaccga aaggcacgac ttggtctcgt
     4981 actggtggct gttccgccgc gtgaagcgcg gatcctcctc cacgtcggtc acgaggaggg
     5041 gaacgccgtg cttggcgacc cagccggcga tggagtcccc catcttgagc cgcgtccccg
     5101 cgacgacctc ttcgcggagg ccgcgcgccg ccttgatgta gagctcctcg cgctcctcgt
     5161 ccaccagcat cagcgaggcg atccgcgcgt gcatcacctc ggcgatcatc tccaccgtga
     5221 ggcgcgtgag gtggtcggag gcggtgcgtc ccgagtccca ccccgcgtgc gtgatcacgc
     5281 ccgcgtcgcc cggcgtcgcc gggagggtga ccgtgaagct cgagcccttc cccggctcgc
     5341 tctcggcgtg gatctcgcct ccatgccact ccacgatgct ccgcgcgatc gagagcccca
     5401 gccccacgcc cggatgctcg cgggagagcg acccgtcgat ctggtagaac ttgtcgaaga
     5461 tcttgtccag ctcctcgcgc gggatcccga tgccggtgtc ctcgacccgg atgcgcgcga
     5521 cccttccttc gccgaagacc cggatccgca ccttgccgcc gcgctcggtg aacttgatcg
     5581 cgttctgccc caggttgagc aggatgcgcc gcaccaggct gcggtcggcc tccaccacca
     5641 cctcgcgcga gggcgcctcc acgtccacgt cgaccccgtc ctccagcgcc atctcgtgga
     5701 gcagcccggc cgactcctcc gccacctcga ccaggttgaa cggctcgcga tgcatgcgga
     5761 agcgccccga ctccagctgc gagagctcga ggatcgattc gatgagccgc ttcagcttct
     5821 ggctctgctc gtggatcacg cccaggaatt cccgctgcat gccgacgttc ggggtgccca
     5881 cgttctcgag gagcgcctcg gcataggaga ggatgtgggt cagcggcgtg cgcagctcgt
     5941 ggctcacgat cgacacgaag gagctcttga gatcgttcag ctcctcggcg cgctcggtag
     6001 cctcctccag ctggcgcatg ctctcgtgca ggcgctcgta cagctccgag ttctggagcg
     6061 cgaccacggc gtgggtggcg aggatttcca gggtctcgac gatctccttg ctcgggcgac
     6121 gccggtccac ggggtcgtcc acggagagat agccgatcac ggtgccgtcc ttggtgcgga
     6181 gcggaacgaa gagcacgtcc tgcgcgttcc actcccccgc tttccgctcg ccgaggtcgg
     6241 gcgtgaaccc gtcctcgtcg cgctcgcccc agaacttcgc ctcatggcca atgaagaagg
     6301 agcgcccgat ccggaacttg tcttgcaacc agctctcgaa ctgcgcgcgc gggaccggct
     6361 tctgctccag cttcatcacg gcctcgcggt cgagacccgc gaaggcccgc gcctcgaagc
     6421 agtcgccgcg cgggttcaga acgcggagca ggacgatctt gaatcccgcc gcgtcggcga
     6481 cggcgtggac gatctcgtgg agcaccttgt cgagcgtcag gttggaattg atgctcgtcg
     6541 cgatctcgag caccttcttg agccgcgccg cgcgcttccg gctggagagg agctcgttcc
     6601 ggtagagcgt ggccatgtcg tcggcggcgc gcgtcagctg ctcgttccgc gcgagcgccg
     6661 actcgtagga cgcgcgggcc tctttgagct cgcgacggag ggagcggcgg cgcccgagcg
     6721 cctcgaagag gaggagggcg gtggccgcga gagcccagag cagctcaacc gagcgcatcg
     6781 ggcctccagg acgcgggctc gcggcgcgct tcggacgcgc gccccttcgc gtcgggcgcg
     6841 gcttcctcgg cgagcaggcg cgcgaactcc tcgaccacgc gcggatcgaa ctgggttcct
     6901 ccgcagcggc ggatctcggc gatcgcttcc gcctcgccca ccggctcgcg gtagggcctg
     6961 cccgccgtca tcgactccca ggcatccacc acggccagca cgcgcgcggc cagcgggatc
     7021 tggtcgccgg ccagcccgcg cgggtagccg cgcccgtccc agtgctcgtg gtgccccagg
     7081 atgatctcgt tcaccttgga cgtgagctcg atcggccgga gcatctggac gccagcctgc
     7141 gggtgtccct cgacgtcgcg ccgctcctcc ggcgaccagc gacgcgccga gaggaggagc
     7201 ccgtcgtcga tctccagcat gcccacgtcg tgcacgcgcg ccacgtagcc aagcacttcc
     7261 gcgtcctcgg ccgagagccc cacccgccgc ccgagctcca ccgccagctt gaaggcgcgg
     7321 cggcttccgg ggaggaggtg cgtccggcgc gcgcgcagga tggcgcggat ggtgcggagc
     7381 gtggcttcga gcgtctcggg cgagacgtcg ccgcggacgc gatccacggc cgccgccacg
     7441 cgcttgacga ccgccgcgag aagaccgaga tcgtccgcgt cgaactccag cccgttccgc
     7501 ttgttgttca cgttcacgac gccgacggtt tcccccgcaa ggcggagcgg cacgcagagg
     7561 agcgacttcg tctcgtactg cgggtggttc atcttccgga accggcggtc ggtttcgatg
     7621 tcggcgacca gcagattctc ggaggtccgc gcgacccacc ccgcgattga atcgcccgcg
     7681 cggacccggg tgcgggccac ggtgtcggcg tcgaggccgt gcgcggcgtg gatcgccatc
     7741 tccgatccgt cctcgctgaa gaacaggacc gagacgatgc cggcgtccat gacctccgcg
     7801 atcatctcga cggagacctg gagcaggttt cgaagctcgg gctgctcgaa cagcggcgtg
     7861 agcgactgga tgagcgcgcg gaacccctgc tgctcccgcg gcaggtccac gaggaagcgg
     7921 cttccgtgtc ccacctcgct ctccaccgtg acggctccgc cgtggagctg cacgacgcgc
     7981 ttgacgatgg ccaggccgag gccggtgccg gcgacgcgct cgcccccctc gggctcgacg
     8041 cggtagaagc ggtcgaagac ccgcgcgatc tgatccttgg cgatgccggg cccctggtcc
     8101 tccacgccga tccggatccg gtccccctgc ccgaaggcgg agacggtgat ggcgctccct
     8161 tcgcgcgaga acttgacggc gttgtggatc aggttcacga gcacctgctt caggagatcg
     8221 ggatcggcct cgatcggcgg gagcgcgggt tcgagggaga gcctgaggcg caggccgcgc
     8281 tggcgaagcc ccggctccac ggtgggcgcg acctcctcga tcagcttggc cagcgaaacc
     8341 agaacggtgt tcaagcggcg ccgtcccgag tccatccggc tcaggtcgag cacgtcgttc
     8401 accatgcggg agaggcggtc gcactcctcg tcgatgatgc cgaggaagcg cttgtggagg
     8461 gggaagctcg cggcgtcggc gttgtcgagc agggtctcgg tgtaggcctt gatcgccgtg
     8521 agcggcgtgc ggagctcgtg gctcacgatg gagatcaggt ccgacttggc ctcgtcgatc
     8581 cgctgcagcg cggcgcgcgc ctcttccagc tggcgcgtct tggtctcgat ctcggcgcgc
     8641 gcgcgcagcc ggtcgagggc cagcccgatc tggtcggcca ggtagcggag cgcgcggcga
     8701 cggcggtcgg tcagcgtgac gctgcggcgg aagcccagca cggccacgcc gaggagggcc
     8761 gtcccgacca cgatcggaac gccgatgacg ctctcgaagg agccgtcgcg ccaggggatc
     8821 gcgttctcgg acgcggtcgc gggtggcggg gtctccacga ccagggcctc gcgccgcagc
     8881 acccgctcga ggaaggcgtt cgatcgcggc gcgtagcgcg cgtggccgac gggcgcccgc
     8941 tcgccgcgct cgaaggcgtc gcctttcatc tcgccggtct cgtagtccag gaggaagagg
     9001 gcgaagccgt ccgcgcgggc gatctcgagg acgtgctggg cgagggcgtt caccagtccg
     9061 cgggtgtcga acgcggactg cgccgcgaag gcgaggctgg cgaggctgcc gaggaggcgg
     9121 ccgcgtccct ggcccccttc ggagatcatg gcgccccgcc cccgcgcgat ccgtcggccg
     9181 gagggtcggg agcggccgga accgcgggag agccgtcact ccggcgatcg aagacgatga
     9241 cgctcccctt gaccgctttg ccgaccttct tcagatcctt cgcgcggtcg ctcacttgcg
     9301 cgctgtgctg gatctcgacc ccgacgttca tgacgcccag gatcgtgatc gagaggatgt
     9361 cgaagtcgcg ccagccgccc tggcggctgg agaggacggt gatctggcgg cgctcgcgat
     9421 ccagcgggtc gtactgctcc ggcacgcggg cgtcgaagtc ggccttgacc gcctcggcga
     9481 tcgcgcgcgc ccgcgcaggc acggtgatga tcacgaagtc gtcgccgccg atgtgcccca
     9541 cgaagtcgcc ctcgccgccg tgctggcgga cctgttcttg aagcagggcc gccgtgaagc
     9601 ggatcagcgc gtcgccgcgg gcgtagccat agtagtcgtt gaacgccttg aaggcgtcga
     9661 gatcgagatg caggaaggcg aacttctccc cggcgccgat ccggcgattg agctcggtct
     9721 cgatgagcac gttgcccgga agccccgtga gcgcgttcga cgagagctgc gcacggcgcg
     9781 tcttcagctg gttgcgaacg cggaagagaa gctcctgctg ctcccacggc ttggtcagat
     9841 agtcgtcggc gtagagctcg aggccgcgca gccggtcgtc cacggtggac ttcgccgtga
     9901 ggaagatgat cggaatgcgg ctcgtgagcg ggttggactt gaggcggcgg cagacctcga
     9961 atccatccat gcccggcatc atgacgtcga gcaggatcag ctccggcggc tcgcgcatga
    10021 cttccgccag ccccttcacg ccgtcctcgg cggtgcgcac ttcgtagcgc gcgtccttcg
    10081 cctcccggag ctggcgctcc aggatgaagc gcaggcgcgg ctcgtcttcg atgaccagga
    10141 tgcggccgga ttcggccggc gacggctccg tcacgaaacc tccgttcgct cgcgatgtag
    10201 gcggaactcg gcttgcgtct tggaattaat cggtggcgaa gcggcggatc ttgagtgggt
    10261 ttacggggtg gggcgttcta agtaacggcc cggagggtga ttcgccgggc gccggcgccc
    10321 gctcgcggcg ccggacccgg aagatcgctg tcaggggaag gttacacggg gctcgcgcac
    10381 gcgcggcact tgcgcgccga ggcggcgatg atctccttgc actcgggaca ctccttggtc
    10441 ggcggtccgg cctccggctt caggagcgcc ttggtgatca tgaagaccac gaaggcgatg
    10501 atcacgaagt cgatgaggtt tccgaggaac gctccatacg tgatcgcgtt cgcgggggtc
    10561 ccgtcgggct ttaccgagag cacccacttc tgctcgcgcc aggctccgcc gggaatcatg
    10621 agtccgatga ccggcatcag gatgtcccct acgagcgagc tgaccacctt gccgacggcc
    10681 gcgccgatga tgacgccgat cgccagcgcc agcgcgttgg tctgcagcag gaacttacgg
    10741 aattcggtga gcatgtcccc tcctccatga gaatgatgag acgcgcggca ttgtgcggct
    10801 ggatcgggga gggtgcaagg gtctatgccg gaaacgaatc caggttgggc accgcggata
    10861 ccgtgaccgg gctgacgcgg ccggccgtga gcgcggccca gccggcgtag gcgatcatcg
    10921 ctccgttgtc gcgacacaaa ggcggcgacg ggaagagcgc cggaatgccg cgcgaggcgg
    10981 cgagcgtcgc ggcgtcccgg cgaagccgcg tgttgcaggc gcagccgccc gcgatcgcga
    11041 gcgcgcgcgc ggggtaacga tccagcgcca gcgatagccg ttcgatgagg gcggtcacga
    11101 tggcgcgctc cgccgaggcg gcgatgtcgc gctcgcgcgc cggcgggatc ggatcgggct
    11161 ccttctccac gagcagcttc accgcggtct tgagcccgct catgctcacg tccagggtgt
    11221 cagccccgag gcgggccacg ggaaatgcga tcgcgtcggg gttcccctcg cgagccaagc
    11281 ggtccacctc cgggccgccc ggatacggca gcccgaggag cttccccacc ttgtcgaacg
    11341 cctcccccgc cgcgtcgtcg cgcgtcgagc ccaggcgaac ggcgcggccg cgctccggca
    11401 cgtgatagat ctcggtgtgt ccgcccgaag ccaccattcc gaggtaggga aacggaaggt
    11461 cgttctcgag cgccggcgac aggaggtggg cctcgacatg attcacggcg gcgagcggga
    11521 tcccgcgtgc catcgagagc cccttcgcga actccagccc caccaggagc gaacccacga
    11581 gcccggggcc cgcggtggcg gccacaccgt ggatgtcggc caagctcaga cccgtcttcg
    11641 cgagaagctc gtccaggatc ttgggaagga gcgtgatgtg cgcgcgcgag gccagctcgg
    11701 ggacgacgcc accgtactca gcgtgcacgt cctgcgcgcg gacgacgtgg ccgagcaggc
    11761 ggccatcctc gacgatgccg gcgccggtgt cgtcacagga ggtttcgatt ccgaggacac
    11821 gcatcgggtg attctagcag gacgccggcg agccatgcgg ctgcctactg gagcgaacgg
    11881 ttgacgttga gcgccgccgc gacgaagaga acggagatcg tgagcatccc gatcgcaaag
    11941 agccgcgcgt ccgtcctccc atttcgatag agcgacagcg aatcgaccga tgcgagggga
    12001 atggtgacgg gcgccctgtg atcggcgcgc atcgtcccgt gaagggtgtc cgctcgcacc
    12061 tcgggatgct ggaccagcag caccgacccg ccggcaccaa ccctggcgta gtcgggcttg
    12121 ctgtcgcgca gggtgtccgc gatgcgttcg cgctggaccg gctgccagtg ggcacagccg
    12181 acttcactca ggagggcgag aagcaggaat acgctcgcgg cagcaggggt gcgatggcgc
    12241 gatgctgaac gagacgcggc cttcatggaa ccacccgatc cggcggggga actaccgtac
    12301 agcttgctct taccgcgccc cgaacgtcaa cgggccggga cctggcgggc gcgcgaactc
    12361 cagaggcagg cggcgaagac gaggaccggg atggaaggcg ccgtagacca gcagggcgcc
    12421 catcaccagg cgccgaagat ggatcgaagt gtttccgccg gcgtgaatgc ggccgtgacc
    12481 atggtgcagg tgagcgcgcc ggagaacagc atgatccact tgatcgccga atcccaggac
    12541 atggcgagcc tccgagcgtg gaaggggccg ggccgctcag gacgcggggc ggctaggcga
    12601 tcggatccgc cgagtcgg
//
LOCUS       DASSLZ010000355         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1758414, whole genome shotgun sequence.
ACCESSION   DASSLZ010000355 DASSLZ010000000
VERSION     DASSLZ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758414"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VFM00_13485"
     CDS             complement(<1..643)
                     /locus_tag="VFM00_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HET7499304.1"
                     /translation="MTRTASEWPALPLDRWRDTYATLHMWTQIAGKICLALTPRMNHF
                     WNIAFQVTPRGLATPTMTAGERTFTITFDLVAHQLLIEVSDGSVRTIRLEPRSVAAFY
                     RLLMDTLHGLGIDVRIWPMPVEIPDPIRFTEDETHHAYDPVCANAFLRALLSIKPVFQ
                     SFRCDFVGKCSPVHFFWGSFDLAVTRFSGRRAPERPEADAMTRESYSHEVISHG"
     gene            667..1908
                     /locus_tag="VFM00_13490"
     CDS             667..1908
                     /locus_tag="VFM00_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018773914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7499305.1"
                     /translation="MAEGKAEGAPERIDTVVIGAGQAGLAASWCLTRRGIEHVVLERG
                     RVGETWRSERWDGFYLNTPNWTLQLPGFPYNGPDPDAFASLRETITYLERYAKSFGAP
                     VRERTPVERVRRDGGGPFLVETSAGSLSARRVIVAAGAYQRPTPTPLARRLPPEIASL
                     HTSEYRRPSQLPGGAVAIIGSGQSGCQIAEELLLAGRRVYLAVGRCPWLPRRYRGREL
                     MRWMIDAGLADQTVDTLPSPAARLGCNVPVSGNEGGHDCNPRWLARHGAVLMGRVEAI
                     EGTLLRLGEGLGQNLAAGDEFVAGFRKLADGVAAASHLELPDPEPVDEPVASPELREL
                     DLREAGVAAVIWANGFRPDHAWIDGVEHDAQGWPVHERGVSPVPGLYFVGLHWLHKRK
                     SALLMGVGEDAEHVVTTLANN"
BASE COUNT          345 a          767 c          763 g          345 t
ORIGIN      
        1 agccgtggct gatcacctcg tgcgagtacg actcgcgcgt catcgcgtcg gcctcgggcc
       61 gctccggcgc gcggcgtccg gagaagcgcg tcaccgccag gtcgaaactc ccccagaaga
      121 aatgcacggg gctgcacttg cccacgaaat cgcagcggaa gctctggaag accggcttga
      181 tcgagaggag cgcgcgcagg aacgcgttcg cgcagaccgg gtcgtaggcg tgatgggtct
      241 cgtcctcggt gaagcggatc gggtcgggga tctcgaccgg catcggccag atccgcacgt
      301 cgatgcccag cccgtgcagg gtgtccatga gcaggcggta gaacgcggcc acggagcgcg
      361 gctccagccg gatcgtccgc acggacccgt ccgagacctc gatcaacagc tgatgggcca
      421 cgaggtcgaa tgtgatcgtg aaggtccgct cccccgcggt catggtcggc gtggccaggc
      481 cccgcggcgt gacctggaag gcgatgttcc agaagtggtt catccgcggc gtgagggcca
      541 ggcagatctt ccccgcgatc tgggtccaca tgtggagcgt ggcgtaagtg tcgcgccagc
      601 gatccagggg tagggcgggc cactccgatg ccgtgcgcgt catgcgcata cgatacactg
      661 ccgcgcatgg cggaaggaaa ggcggaaggc gcgcccgagc ggatcgacac ggtcgtgatc
      721 ggcgccggcc aggccggcct tgcggcaagc tggtgcctga cccggcgcgg gatcgagcac
      781 gtggttctgg agcgcggacg cgtcggcgag acctggcggt cggagcgctg ggacggcttc
      841 tacctcaaca cgccgaactg gacgctgcag ctccccggct tcccctacaa cggccccgat
      901 ccggacgcct tcgcgtcgct tcgggagacg atcacgtacc tcgaacgcta cgcgaagagc
      961 ttcggcgccc ctgtccggga gcggacgccg gtggagcgcg tgcgccgcga tgggggcggg
     1021 cccttcctgg tggaaacgtc cgccggatcc ctatccgcgc gccgggtgat cgtggccgcc
     1081 ggcgcctacc aacgccccac gccgacgccc ctcgcccgcc ggctcccgcc ggagatcgcg
     1141 agcctccata ccagcgagta ccgcaggccg tcccagctcc ccggaggggc cgtcgcgatc
     1201 atcggcagcg gccagtccgg ctgccagatc gccgaggagc tgcttctggc ggggcggagg
     1261 gtctacctcg ccgtcggccg ctgtccctgg cttccgcgcc gctaccgcgg ccgcgagctg
     1321 atgcgctgga tgatcgacgc ggggctcgcc gatcagaccg tggacacgct cccttccccg
     1381 gcggctcgac tgggatgcaa cgttcccgta tccgggaatg agggcggcca tgactgcaac
     1441 ccgcggtggc tcgcgcgcca cggcgcggtg ctcatggggc gggtcgaagc gatcgaggga
     1501 accctgcttc gtctaggcga agggctgggc cagaacctgg cggccggcga cgagttcgtc
     1561 gcgggcttcc gaaaactggc cgacggcgtc gccgccgcgt cccatctgga gctgcccgat
     1621 cccgagccgg tcgacgagcc ggtggcgtcc ccggagctcc gcgagctcga cttgcgcgag
     1681 gccggcgtcg ccgccgtgat ctgggcgaac ggattccggc cggaccacgc ctggatcgat
     1741 ggggtcgagc acgacgcgca aggatggcca gttcacgagc gcggcgtctc gccggtgccc
     1801 gggctctact tcgtgggtct ccactggctg cacaaacgga agtccgcgtt gctgatgggg
     1861 gtgggagagg acgccgagca cgttgtaacg actctagcaa ataactaaat tgcttggtgt
     1921 tacggagaca tattcatctg aaatgtagac ccacccgcct gcccgccatt cccctatcct
     1981 cttagggcag cccgcagcac cggccgcatt ggaaatgccg cagctggttc acgcaaggag
     2041 cccccgccat ggacatcttg gcctcgccca ggcgccctca tacatcgttc gctggagctt
     2101 tgctcgccgt cgtatcgctg gtccttctcc cggtcccgtc cgccaccgcg ttctccatcc
     2161 cccccaatac ctgggtcgct cagaaagcgc ccacgcaggt cctgccctcc ggacttgcgg
//
LOCUS       DASSLZ010000356         5801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1879733, whole genome shotgun sequence.
ACCESSION   DASSLZ010000356 DASSLZ010000000
VERSION     DASSLZ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879733"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VFM00_13495"
     CDS             complement(<1..1147)
                     /locus_tag="VFM00_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499306.1"
                     /translation="MAMERDYYFRRIVALALALVVVASAATAAAQYMYLDANGDGVHT
                     EADVFPSAGTATLDVWLVTDKNRDGSSATCAVDPSSALTINSYSFMLRATQGTVVWSG
                     FQDRLLPDAYAAGGESSSTEFWIGRAGITPLAPGRYRLASLTVTATFGSPSILIVSTS
                     QTMSGAPLTSFGSQCMAADYDNTMKLGTDWFDADGVPFSAVGNGAPALEPIPAITVPE
                     GSVVERTLHATDPDGQPLTFGVDGPPYVSVTTTDPGAGSATGLLRVAPGYTDAGAVEA
                     IVFASDGTSQAGGLLQITVTDVNRAPVLGAPADMTVAEGKSATQEIRATDADGTTPLS
                     LSIASGPAFLTLSRPVGDMRSITANLTLAPGYSDEGSYTGAVTASDGM"
     gene            complement(1221..1622)
                     /locus_tag="VFM00_13500"
     CDS             complement(1221..1622)
                     /locus_tag="VFM00_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499307.1"
                     /translation="MLLLTTLVVALATSAIVVALFRRPIRQILDRIIGPELAHAWQRF
                     LLFAIYVVGVSSGVQIWKLEQYIRAEPGPNGAGRPPFVLNGESMGLEVYRTILQVLQG
                     IAWALLVFFVVALIAFVLVRRGEAQRERGAS"
     gene            1783..2304
                     /locus_tag="VFM00_13505"
     CDS             1783..2304
                     /locus_tag="VFM00_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET7499308.1"
                     /translation="MIDKLDLAILSILQENARTSNVEVARRIGLAPSAALERIRKLEK
                     RGVIRGYEPRLNAAAVGSPLLAFIFVHADEKIGGLEIGRKIAKFPEVQEVHQVAGEDC
                     YLVKVRIADTDALGRLLRDSFGSLPAIRTTRTTIVLSSVKESAQMPLPPSGASGPRER
                     VPKVPRRGRRRNP"
     gene            2301..3245
                     /locus_tag="VFM00_13510"
     CDS             2301..3245
                     /locus_tag="VFM00_13510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7499309.1"
                     /translation="MTSAALRASRFKLVLAFACIYFIWGSTYLAIRFAVETMPPLLMA
                     GVRFLIAGGLLYGWLALRHETRRPTREEWKAAAILGVLFFLGGNGAVSWSETRVPSGL
                     AALLVATIPFWIVILDWMRPGGMRPAAAVLAGVAAGFAGVALLVGTHGPEGGIDHAGA
                     AVLTAGPIAWAWGTVISRRMKHPRSHLQSGAMQMLAGGLALCIGGLALGEARQVRLDA
                     ISMRSVLAALYLVFAGSIVAFSAYNWLLYASTPARVGTYAFVNPVVAVFLGWLFAGEA
                     FGPNTLTAALFILAGVVLIVVSRMRIKNGRAVTVPADA"
     gene            complement(3335..4216)
                     /locus_tag="VFM00_13515"
     CDS             complement(3335..4216)
                     /locus_tag="VFM00_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499310.1"
                     /translation="MNRTASLFVTLGVLCFAAAPALADDAATQTAPGAADQTQTPATQ
                     PAPVTQPAPATQPPPATQPAPATTPAPADPGQPAVGTEAAAPAAPSASDVLAKIRDEG
                     KKVDAKTDQKVNAALDAASNDVEKNAATDGDQKIAARLAAEFGTTPDALLSEKSDVKA
                     SWGQLMIAHSLLANGAVDLTTKQIFDLRGEGMSWGQIANGMGLRLGDVVKAAKEEARV
                     AKGVSPPDGKVAVVHGAGSKFEASARSAKAEKAEKTAMKEAAKAESAKADAAKEAAPD
                     AAATPAGSAAGGDGGKK"
     gene            complement(4622..>5801)
                     /locus_tag="VFM00_13520"
     CDS             complement(4622..>5801)
                     /locus_tag="VFM00_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HET7499311.1"
                     /translation="NVHVVAPQAPFARIVAPAVAYVGVDATFDGSLSYDPDGDLLHYG
                     WDFGDQSVLSGIKVQHRYGATGVYTVTLTVGDGLLSDTATRQVTVNAIPHPPKAMAGG
                     PYSGLMGRLISFDGTRSSDPDGDPLLYMWSFGDRSTGSGPAPGHVYDAPGVYTVSLSV
                     SDGGLTASATTSATVKESLPARVFANGSALYTPGQSEPMVLHLESADRSYGIEDIQTW
                     PISMRTDGTGTMDEIACVADATITPDSDGNRAPELTFQFSPEELALLFAHTAVGTTVT
                     AHLRGDFARGGHFTAELPIRVDNAEAAGPLRISPNPFNPEAVVTFSTTKPGPVRAQLF
                     NVHGRLVRTILYESMTSGRHVVTLDARNDVGRILASGIYFFRLTGPDGVSTKRVAIAK
                     "
BASE COUNT          879 a         1936 c         2062 g          924 t
ORIGIN      
        1 ccattccatc gctcgcggtg acagcccccg tgtaggagcc ctcgtccgag tatcccggtg
       61 ccagcgtcag gttggcggtg atgctccgca tgtcgccgac gggacgggac agcgtcagga
      121 acgcgggccc cgaggcgatc gagaggctca ggggcgtcgt cccatccgcg tccgtcgcgc
      181 ggatttcctg cgtcgcggac ttcccctcgg ccacggtcat gtccgccggc gccccgagca
      241 ccggagcgcg gttcacgtcg gtgacggtga tctggagaag acctcccgcc tggctcgtgc
      301 cgtcgctcgc gaagacgatc gcctcgacgg cgcccgcatc cgtgtagccc ggcgcgacgc
      361 gcagcaggcc ggtggcgctt cccgccccgg ggtcggtcgt cgtcaccgag acgtagggcg
      421 gcccgtcgac gccgaaggtg agcggctggc cgtcgggatc ggtggcatgc agcgtccgct
      481 ccacgacgga cccctccggc acggtgatcg ccgggatcgg ctcgagcgcc ggtgccccat
      541 tccctactgc cgaaaacggc acgccatcgg cgtcgaacca gtccgttccc agcttcatcg
      601 tgttgtcgta gtcggcggcc atgcactggg agccgaagga ggtcagcggc gcgccgctca
      661 tggtctgcga ggtcgacacg atcaggatcg acggcgagcc gaacgtcgcg gtgaccgtga
      721 gactcgccag ccggtaccgg cccggcgcga gcggggtgat gcccgccctg ccgatccaga
      781 actcggtcga cgacgactcc cctcccgccg cgtaggcgtc gggcaggagc cggtcctgaa
      841 agccgctcca gacgaccgtt ccttgcgtcg cccgaagcat gaacgaatag gaattgatcg
      901 tgagcgccga ggaaggatcc acggcgcagg tcgcggacga gccgtcgcgg ttcttgtccg
      961 tcacgagcca gacgtcgagc gtggccgtgc ccgccgaggg gaagacgtcg gcttcggtgt
     1021 ggacgccgtc gccgttggcg tccaggtaca tgtattgggc cgcggcggtg gcggcgctgg
     1081 cgacgactac gagggcgagg gcaagagcga cgatacggcg aaagtagtaa tcacgctcca
     1141 tagccacctg actttccggc cggcgccggc tcgtcggcat gggtggcgac cgaaaggatg
     1201 aagcgcgggg tgcgcggtcg tcatgacgcg ccccgttccc gctgcgcctc gccgcgccgt
     1261 accaggacga acgcgatgag cgccacgacg aagaagacga gcaaggccca ggcgatcccc
     1321 tgaagcacct ggaggatcgt ccggtagacc tcgagcccca tggactcccc gttcaacacg
     1381 aacggcggcc gcccggcgcc attgggaccg ggctccgcgc ggatgtactg ctcgagcttc
     1441 cagatctgaa cgccggagga gactccgacg acgtagatgg cgaagaggag gaaccgctgc
     1501 caggcgtgcg ccagctcggg gccgatgatg cggtcgagga tctggcgaat ggggcggcgg
     1561 aacagcgcga cgacgatcgc ggaggtggcg agcgccacca ccagcgtcgt gagcagaagc
     1621 atgaggaaca ccgtggctta cccccttgga gccgcggacc gcgcgatccc gtttcagtct
     1681 acgcccgagg aaggacccgg acacccggaa ttcccccgca agaatcggag tgcgttaagg
     1741 taccttaacg aatataattc ggttctagcc tggaggtacc aaatgatcga caagctagac
     1801 ttggcaatac tgtcaattct tcaggaaaac gcccgcacgt cgaacgtgga ggtggcgcgg
     1861 cgcatcggcc tggccccctc cgccgccctg gaacggattc gaaagctgga gaagcgcggc
     1921 gtcattcgcg gctacgagcc gcggctgaac gccgcggccg tgggctctcc gctcctcgcc
     1981 ttcatcttcg tccacgccga cgagaagatc ggcggactcg agatcggacg gaagatcgcg
     2041 aagttccccg aggtccagga ggtgcaccag gtggcgggcg aggactgcta cctggtcaag
     2101 gtgcgcatcg ccgacaccga cgcgctgggg cgcctgctcc gcgactcgtt cggctccctc
     2161 cccgcgatcc gcaccacgcg cacgacgatc gtgctctctt ccgtgaagga atcggcgcag
     2221 atgccgctgc cgccgagcgg cgcttcgggc ccgagggaac gcgtgccgaa ggtgcccagg
     2281 cgaggccgcc ggaggaatcc gtgacctccg cggcgctccg cgcctcgcgc ttcaagctgg
     2341 tgctcgcgtt cgcctgcatc tacttcatct ggggcagcac ctatctggcg atccgattcg
     2401 ccgtcgagac gatgccgccg ctgctgatgg ccggggtgcg cttcctgatc gcgggcgggc
     2461 tcctctacgg ctggctcgcg ctccggcacg agacgaggcg ccccacgcgc gaggagtgga
     2521 aggccgctgc gatcctcggc gtgctcttct tcctgggcgg aaacggagcc gtgtcgtggt
     2581 cggagacccg cgttccttcg ggcctcgcgg cgctcctcgt ggcgaccatc cccttctgga
     2641 tcgtgatcct ggactggatg cggccgggcg gcatgcgccc cgcggccgcg gtgcttgccg
     2701 gcgtggctgc gggattcgcc ggtgtcgcgc tcctcgtcgg cacgcatggg ccggagggcg
     2761 ggatcgacca tgcgggcgcc gccgtgctca cggcggggcc gatcgcgtgg gcgtggggaa
     2821 cggtgatctc gcggcggatg aagcatccgc ggtcccatct ccagtcggga gcgatgcaga
     2881 tgctggcggg aggcctggcg ctctgcattg gggggctcgc gctgggcgaa gcgcggcagg
     2941 tgcgcctgga cgcgatctcg atgcggtcgg tactggcggc cctctatctc gtgttcgcgg
     3001 ggtcgatcgt cgcgttcagc gcctacaact ggctgctcta cgcatcgacg ccggcgcgcg
     3061 tgggaacgta cgcgttcgtc aatccggtgg tggccgtctt cctcggctgg ctgttcgcgg
     3121 gcgaggcgtt cgggccgaac acgctgaccg cggctctgtt catcctcgcg ggggtcgtgc
     3181 tgatcgtcgt ctcgcgcatg cggatcaaga acggcagagc cgtgaccgtg ccggccgacg
     3241 cctagcaaag caaagggcgg acccgtaggt ccgcccttcg agccgcgacc ggcccttccg
     3301 tgctcggccg cggtgcttta ctgctgagcg ccggctactt cttcccgccg tcgccgccgg
     3361 cagcgctgcc ggccggagtc gccgcggcgt cgggagccgc ttccttggcc gcgtccgcct
     3421 tggcggactc cgccttggcc gcttccttca tcgccgtctt ctcagccttc tcggccttcg
     3481 cggagcgggc gcttgcctcg aacttggagc cggcgccgtg cacgacggcg accttgccgt
     3541 ccggcgggga aacacccttg gccacgcgcg cctcttcctt ggcggctttc acgacatcgc
     3601 ccaggcgaag tcccatgccg ttggcgatct gtccccacga catcccctcc cctcgcagat
     3661 cgaagatctg cttggtcgtg aggtcgacgg cgccgttggc gagaagcgag tgggcgatca
     3721 tgagctgccc ccagctcgcc ttgacgtcgc tcttctcgga gagaagcgcg tccggcgtgg
     3781 taccgaattc agcggccagc cgcgcggcga tcttctgatc gccgtccgtc gcggcgttct
     3841 tctccacgtc gttggaggcc gcgtccaacg ccgcgttcac cttctggtcg gtcttggcgt
     3901 ccaccttctt cccctcgtcg cggatctttg ccaggacgtc cgaggcgctc ggcgccgccg
     3961 gggccgctgc ttcggtaccg acggcgggct gacccggatc ggcgggagcg ggcgtcgtcg
     4021 cgggcgcggg ctgcgtggcc ggaggcggct gcgtggcggg cgccggttgc gtgacaggcg
     4081 ccggctgcgt ggcgggcgtc tgggtctggt ccgcggcgcc gggcgccgtc tgcgtggcgg
     4141 cgtcgtcggc aagggccgga gccgcggcga agcaaaggac gccgagggtt acaaacaggg
     4201 aagcggtgcg attcatcgtg ctgactcctt catggccgcg cgtcccgaac gcacgtccga
     4261 ggcggcgacc cacggtctcc gcggacacaa aaaaaatggc cagggtcctc gcgcgcccgc
     4321 cccggagagg cgagcgcgga ggagatgccg tgatcgaact acgttccaac cctggccgcc
     4381 tcgattcttc ccggcgccgt catggcgacg ccgaggcaaa ccatctagat caacggacat
     4441 gccagaggaa ggggcggcgc cggtgaaaag cacaacccgg gagcacgagg cgatttagcg
     4501 gcgaggcgag ctggagcggc gccaagcgaa aggcgccgct ctgtcccgtt gcggacagga
     4561 agcggcgccc ctggaacggc tcgggacggc tgtgcggctc gggctcgccc gatccgtccc
     4621 tctatttcgc gatcgcgacc ctcttggtcg agactccgtc gggccccgtg agacggaaga
     4681 agtagatgcc ggaggcgagg atgcgcccca cgtcgttccg cgcgtcgagg gtcacgacgt
     4741 gacggccgga tgtcatggac tcgtacagga tggtccgcac gagacggcca tgaacgttga
     4801 agagctgcgc ccggaccgga cccggcttcg tggtcgagaa cgtgaccaca gcctccgggt
     4861 tgaacggatt gggcgagatc cgaagcggcc ccgccgcctc ggcgttgtcg acacgaatcg
     4921 gaagctcagc cgtgaaatgg ccgcccctcg cgaaatcccc ccgtagatgc gccgtgaccg
     4981 tcgttccgac cgccgtatgg gcaaagagaa gcgccagttc ctcgggactg aattggaagg
     5041 tcagctccgg agcccggttg ccgtcggagt cgggcgtgat cgtggcgtcg gccacgcagg
     5101 cgatctcatc catggtgccc gtcccgtccg tacgcatcga gatgggccac gtctggatat
     5161 cctcgatgcc gtaggaccgg tcggccgact cgagatggag gaccatcggc tcgctctgcc
     5221 ccggcgtgta gagggccgag ccgttggcga acacgcgggc cggcagcgat tccttgacgg
     5281 tcgcggacgt cgtggcggag gcggtgagcc cgccgtcgct gacgctgaga ctgaccgtgt
     5341 acacgcccgg ggcgtcgtag acgtggcccg gcgcgggccc ggatccggtc gagcgatcgc
     5401 cgaagctcca catgtagagc agtggatcgc cgtccgggtc ggacgagcgg gtgccgtcga
     5461 acgagatcag ccgtcccatg agtccggaat agggaccgcc cgccatcgct ttcggcggat
     5521 gggggatggc gttgacggtg acctggcgcg tggcggtgtc gctcaagagg ccgtcgccga
     5581 cggtcaaggt cacggtgtag acgccggtgg cgccgtagcg atgctggacc ttgattccgc
     5641 tgaggacgga ctggtcgccg aaatcccacc cgtagtggag caggtctccg tccggatcgt
     5701 aggacagcga tccgtcgaag gtggcgtcca cgccgacgta ggcgaccgcg ggcgcgacga
     5761 tccgcgcgaa cggcgcctgc ggcgccacaa cgtgcacgtt c
//
LOCUS       DASSLZ010000357         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_250624, whole genome shotgun sequence.
ACCESSION   DASSLZ010000357 DASSLZ010000000
VERSION     DASSLZ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250624"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1845
                     /locus_tag="VFM00_13525"
     CDS             <1..1845
                     /locus_tag="VFM00_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499312.1"
                     /translation="DSAPRTPSSLVAGVDPVVERVILRCLERDPSRRPQSAYAVFGAL
                     PGGDPLAAAVAAGETPSPELVANAGVEGSVRPLYAGLLVLIAVAGLLGVSAIQTPMFR
                     GIGKPPDLLSVRADEIVTRLTGEAPPKYTLDGMRYPPKPDTARAVQFWKRWSPMPYVP
                     SDLHVPRAGLQDPPQAYPGSATSLLDLQGRLIALDALRPDSLAAAPPRTADWGGAVLD
                     AAGRDPDHVVGVTPPSTFFARADSVRAWRLSDSTAPETTLVAAALRGRVVRVETYAQG
                     QPLGRLAVAAHDRAPTAQVWVQVLLFAVVPLIGSIILARHNLRSRRGDLRGAMAMGIS
                     TVVLYLLAHLFSGNLGEIGLLSVLIGGTGGMPLGHALVHGVTMTLAYLAIEPYVRRLW
                     PSVLVSWARLVAGRLRDPIIGRDVLIGVAFGSATALIGPGMQVLEQRLGFPIDPPSLS
                     SVLLETMTHPPSLLANVCAALAVGILRATLFFTILVIFRFVLRKNVLAAIATILCFIF
                     ALSDLSSKALALDLLATAVIFTAGTLMSLRFGYVATLVSLFTGLLVESQAFSLDFSSW
                     VAPQTTFAWVIVLALVGYGFLTAVAGHSLFRDPLSDPVATAVRSRRAP"
     gene            1873..>2672
                     /locus_tag="VFM00_13530"
     CDS             1873..>2672
                     /locus_tag="VFM00_13530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7499313.1"
                     /translation="MRTTPKDSKARPAAPEAGLFRRPWAAPLALLLLTVLVYARSLGT
                     PIHDWDDNIYFFRDARVEQPSAENLSRILTQPFFSNYHPVTTLTIAFDRAVWGTWVPG
                     FHLTQLFFYAGGVLGIYFLFAALLRRRAEAFAAAAIYAVHAVHVESVAWLASRKDVVC
                     LFFYVFGLLAYVRYADGAPHRWRAYALSILFAGLAMLSKGYAVILPALYVAYDLCFAP
                     RVGRRQILDKIPFVALTAAMTLLTVYAQDKQSGLVDLSLTLGQRILRLG"
BASE COUNT          330 a          972 c          860 g          510 t
ORIGIN      
        1 gacagcgcgc cgcgcacccc gtctagcctg gtggccggcg tcgaccccgt ggtcgagcgc
       61 gtcatcttgc gatgcctgga gcgcgatccc tcgcgccgcc cccagtcggc ctacgcggtc
      121 ttcggcgcgc tccccggcgg agatccgctc gcggccgcgg tcgccgcggg ggagacccct
      181 tcgcccgaat tggtcgcgaa cgccggcgtc gaggggagcg tccggccgct gtacgccggt
      241 ctcctcgtgc tgatcgcggt cgcggggctt ctcggcgtct ccgcgattca gacccccatg
      301 tttcggggga ttggtaaacc tcccgacctc ctctcggttc gtgccgatga gatcgtcacc
      361 cgtctcacgg gggaggcccc gcccaagtac accttggacg gaatgcgcta tccgccgaag
      421 cctgacacgg cacgcgcggt gcagttctgg aaacgatgga gcccgatgcc ctacgtgccg
      481 tcggacctcc atgtcccgcg ggccggatta caggacccgc cccaggccta tccgggcagc
      541 gccacatccc tgctcgatct acaggggcgt ctcatcgcgc tggatgcgct gcgacccgac
      601 agcctggccg ccgcgccgcc gcgaaccgcg gattggggcg gcgccgttct cgatgccgcc
      661 gggcgcgatc ccgatcatgt cgtcggggtc acgccgccct ccacgttctt cgcccgtgcc
      721 gactccgtcc gggcctggcg cctatcggac tcgaccgccc ccgagacgac ccttgtcgcc
      781 gccgcgctgc gggggcgcgt ggtacgggtg gagacgtacg cccagggaca acccctgggc
      841 cgtctcgcgg tggctgccca tgatcgggcg cctacggccc aggtatgggt tcaggtcctg
      901 ctctttgcgg tggttccact gatcgggagc atcatcctcg cgcggcacaa tctccgctcc
      961 aggcgcggcg atttgcgcgg agcgatggcg atggggatct ccactgtggt cctctatctc
     1021 ctcgcgcatc tcttctccgg gaatctcggg gaaatcggcc tactctcggt cctgatcgga
     1081 gggaccggag gcatgccgct gggccacgcg ctggtgcacg gcgtcacgat gacgctcgcg
     1141 tatctcgcca tcgagccgta cgtccggagg ctatggccca gcgtgctcgt gtcgtgggca
     1201 cggctcgtcg cgggccggct acgggatccg atcatcggac gtgacgttct gatcggggtg
     1261 gcgttcggat cggcgaccgc gctgatcggt cctggcatgc aggttctgga gcaaaggctc
     1321 ggtttcccga tcgatccgcc gagcttgagc agcgtcctcc tcgagacgat gacgcatccg
     1381 ccttcgctgc tggccaacgt gtgcgcggcg ttggcggtgg gaatcctccg cgcgaccctg
     1441 ttcttcacga ttctggtcat cttccgcttc gtgctgcgca agaacgtgct ggcggcaatc
     1501 gccaccatcc tgtgcttcat tttcgccttg agcgacttga gctccaaggc gctagcgctc
     1561 gacctgctcg ccacggccgt catcttcacg gcggggaccc tgatgtccct gcgcttcggc
     1621 tacgtcgcga ccctcgtctc gctcttcacc ggcctgctcg tcgagagcca ggccttctcg
     1681 ctcgatttca gctcctgggt cgcgccgcag acgacgttcg cgtgggtcat cgtgctggcg
     1741 ctcgtcggct atggattcct caccgccgtg gccgggcact ccctgttccg cgaccccttg
     1801 agcgatccgg tggcgacggc ggttcggtcg aggcgcgcgc cctgacgggg cgctgcccta
     1861 ggatgctccc cgatgcgaac gaccccgaag gactccaagg ctcgacccgc cgcacctgaa
     1921 gcggggctgt tccgccgacc ttgggccgcc ccgctcgcgc ttctcctcct cacggtcctg
     1981 gtctacgcgc gctcgctggg cacgccgatc cacgactggg acgacaacat ctacttcttc
     2041 cgcgacgccc gcgtggagca gccctccgcc gagaacctga gccgcatcct cacccagccc
     2101 ttcttctcga actaccaccc ggtcacgacg ctgacgatcg cgttcgatcg ggcggtctgg
     2161 ggaacgtggg tgcccggctt tcacctgacg cagctcttct tctatgccgg cggggtgctg
     2221 gggatctact tcctctttgc ggcgttgctc cgccggcgcg ccgaggcctt cgcggccgcg
     2281 gcgatctacg cggtgcacgc ggtccatgtc gagtcggtgg cctggctcgc gtcgcggaag
     2341 gacgtggtct gcctcttctt ctacgtgttc gggctcctcg cctacgtccg ctacgccgac
     2401 ggcgcgccgc accggtggcg ggcgtacgcc ctctcgatcc tgttcgcggg cctggccatg
     2461 ctgtcgaagg ggtacgccgt gatcctcccc gcgctctacg ttgcctacga cctctgcttc
     2521 gctccgcgcg tgggccgccg ccagatcctg gacaagattc ccttcgtggc gctcacggcg
     2581 gcgatgaccc tcctgaccgt ctacgcccag gacaagcaga gcgggctggt cgacctctcc
     2641 ctcacgctgg ggcagcgaat cctgagactc gg
//
LOCUS       DASSLZ010000358        12067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1762668, whole genome shotgun sequence.
ACCESSION   DASSLZ010000358 DASSLZ010000000
VERSION     DASSLZ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12067
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762668"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3130)
                     /gene="smc"
                     /locus_tag="VFM00_13535"
     CDS             complement(<1..3130)
                     /gene="smc"
                     /locus_tag="VFM00_13535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET7499314.1"
                     /translation="MFLKRLDIQGFKSFPQRVRVDFGPGITAVVGPNGSGKTNIVEAI
                     RWVLGEQNMRHLRGDTLEDVIFTGSAQRKQLGMAEVSLTMFNNRGVLPSEYTELAIAR
                     RTFRNAQSEYFINKSLCRLRDVRDLFLDTGMGSHAYSLIERGMVDNVLSDESGHRRFL
                     FEEAAGIMRYKTRKKEALAKLDLTLTDLTRVNDVVGEIEREVRSLARQVGKARKYGRL
                     KDEIRSIDLGLAKEEYDRLTSGSGGLRAERQSLEQRRAERAGHLARHEAELEELKLEL
                     LKREGEVRLAQEALSEHEARGAALLNEVAVLRERRSNLAEKLEHARSESSRLRASVEE
                     VRAAAVRLAEEQVRLEQVQAEKGSEEEALAVRLAQLGPVLEERRAALGTRKQLSLDLF
                     QARVEQESSARSLRTKEEDLSRRKDELAAQLSSIANEEETLRAMLADVARSIETSALD
                     VEEAKRGVQRAVEAIAQVERRLLAQDETEKRAREAHAALESRHATLTELKNSYEGYDA
                     SVRWLVAGKARPDRILGTVGDVLHASGEWLVALEAALGEAVQFIVAERTDAALAALTA
                     LEESGEGRATFIALDRISRARVAPIPEEVLSAPGVRGPLIEHVRFEPGYITLASFLLS
                     GIIVVDSVRTGLDLILKHPEQRLHFVTETGERIVAPGIIHGGSGATRAGSVLRREDEI
                     LELAASIERSSAALAETLGASEKLRAERAEAQAAQEGAARALASVEEAHRALEGRRAE
                     QTIRGDAMAKASKSLVDALRSLEEEAARTREAAEAAEEKLRSAEAERGRVDDMLAQEE
                     AEFRATEQERDRLTAQHAETRIEAARSRAAYDLARREIERMRQSADEAERDIQSRDEE
                     ASETERRIEEATTLCARREEELAEEIRLKAEKELVLHGTRERFGAVKGNVDDIETRTR
                     DFRREHQELTERLHRIELEQTESEGAARRLIERIWNEYELDLAAYVPAVPVEGEGAVE
                     IEEIDVADVEEGAVAMSAEAAEPPMAPAAVSSTSVVDLASPEGRRDRLRQLQEKLRGL
                     GPVNLLAVQDYEE"
     gene            complement(3134..4834)
                     /locus_tag="VFM00_13540"
     CDS             complement(3134..4834)
                     /locus_tag="VFM00_13540"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET7499315.1"
                     /translation="MAAVETYPERYANPADFAREVNLPLLGSARVRPVPAGAGLAQLL
                     DISSGAEVLGPVVDSLNETRRSLTLRSLLVAGFPHDPECFATALCLAREWSRRGLRTA
                     VIDLDFWNPTVSRPPSHPNEGLVDVLEYGCSFGRVAWEIVADKLWLIGPGSHPPEESR
                     VAEHPDWVRAARVFASCVDVAIYSAPLLDRRGFTGKLSKKMDAVILAAATERIGRADL
                     RDAFLELWASDAPMIGCVGIEPADAPTASRAEAASGHARVSALAPALESPAPMSPEPP
                     AWEREASAALYAAEVARAAAPASPAPAARATRMEDRGAERDLEAALERDVRRGIVEKK
                     PAAKRGNLLVIAGAVLGLALVAGGLSALLSKRPAPPRGSVQANQPAGEEPVTPAPAPD
                     ERGNLDDPSAMVGAPATPGSGQIGSVTAMGKEIQSAETPAAGSSTPPPAGAPATPSAA
                     SPSATARPPAREALRAGLGGTGTGSLAGIGTGAGDAAKPFRVHVASFRTETKVREIVA
                     ALRAKKLDAWFEPPPPGGDWYRVFVGRFATEAEAATYAQWLIKSGAVDRAQSYPQTKR
                     "
     gene            complement(4835..6253)
                     /gene="selA"
                     /locus_tag="VFM00_13545"
     CDS             complement(4835..6253)
                     /gene="selA"
                     /locus_tag="VFM00_13545"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HET7499316.1"
                     /translation="MKPKRGATNDALRALPSVESLLSLPPLQALAGKAPHALLVGAVR
                     EAIGRARDQIRRGAAPAPPSAHALAAEAAAAAEARLRPSLRSVLNATGVVLHTNLGRA
                     PLAEEAALAAAEAARRYTNLELDLDSGSRGSRMAHLEPLLREILGAPAALVVNNNAAA
                     MLLALNTLARDREAVVSRGQLVEIGGSFRIPEILERAGARLREVGTTNKTRLADYERA
                     IGPATGAILRVHPSNFAIVGFSQEVTREDLVRLARKKKVPLLEDVGSGALVDFRPYGL
                     PEEPRLAEALAQGVPLACASGDKLLGGPQAGILAGTKALIAACRANPMTRALRVDKMT
                     LAALETTLRIYRDPDRREAAIPVLRMLGESAASVRARARRVLRALGAERARRTGAEVI
                     PCSGEVGGGAMPLARVPSFALALRAPRGRAETLARALRLGPDPVLARIEAGRVLLDMR
                     TVPGPDVPRLVAVVARVLDEEG"
     gene            complement(6326..7036)
                     /locus_tag="VFM00_13550"
     CDS             complement(6326..7036)
                     /locus_tag="VFM00_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7499317.1"
                     /translation="MLALEDLHVHYGGIRALKGISIRIEAGQIVTLVGANGAGKSTTL
                     RAISGLIRPSQGSILLDGKSIVGVPAHKIVRLGISHVPEGRIVFANLSVADNLEMGAY
                     LRKDRKGIEEDRRKVFTLFPRLQERERQSSGTLSGGEQQMLAIGRALMARPKILLMDE
                     PSLGLAPLLVREIFGVIREIRERGTTIVLVEQNARMALAVADVGYVLETGVIRLSDKA
                     EALLANPFVQEAYLGGAA"
     gene            complement(7039..7797)
                     /locus_tag="VFM00_13555"
     CDS             complement(7039..7797)
                     /locus_tag="VFM00_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015592550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7499318.1"
                     /translation="MLRLDHVTRAFGGLKAVSDLDLALEPGVLAGLIGPNGAGKTTVF
                     NLITGVYAPTSGKILLEGAPIHGLATSRVAARGVSRTFQNPRLFAELTCYDNVRIACH
                     LHCGATVADSIVRSGRSEREEAAIAVRTRELLESFGLSAYAEEKARNLPYGDQRRLEI
                     ARALAAEPRLLLLDEPAAGMNPQESLQLMKLIREIRESYGLTVLLIEHDMRVVMGICE
                     RITVLDHGVKIAEGLPEAIRRDPKVIEAYLGEEA"
     gene            complement(7857..8852)
                     /locus_tag="VFM00_13560"
     CDS             complement(7857..8852)
                     /locus_tag="VFM00_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7499319.1"
                     /translation="MLRRLLTGAAVLVVVWLLDRFLSGVINPYFYRILVLSGLNVMLA
                     LSLNLINGVTGQFSIGHAGFMAVGAYTSAAFTVYAVPQLLGGNAPQGLVAGLFFIVAI
                     LIGGLIAAAAGFLVGLPSMRLRGDYLAIVTLGFGEIIRVAILNIDAVGGARGFAGIPQ
                     ITTLSWVLAGAILTFVVIQNLLRSYHGRALLAIREDEIAAEALGVPTTRYKVTAFVIS
                     AFFAGAAGALFSHYTYLHTNSFTFMKSIEVIVMVVLGGMGSLTGSILGAVLLTALPEA
                     LRFAAADRLIIYSALLIVLMIARPQGILGHREFSLPRMFGRFKPPSPEPGGSGAK"
     gene            complement(8854..9759)
                     /locus_tag="VFM00_13565"
     CDS             complement(8854..9759)
                     /locus_tag="VFM00_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7499320.1"
                     /translation="MQELFQQLANGIAWGSIYALIALGYTMVYGILRLINFAHGDIYM
                     VGAFTAYFAARALGAGGATPSPAYAVVVLLAAMAACAVLGVLIEFFAYRPVRRSSRLT
                     ALITAIGVSLLLENLGIRIFGADPKFFPQLIAPKNIDLGGGVMVTNHQITVVVVSLVL
                     MVGLTLFIARTRTGKAMRAVAFHRDAASLMGIPVNRIITITFAIGSALAAAAGVLVGL
                     TNPKIEPLMGIMPGVKAFVAAVLGGIGSVPGAVIGGVVMGVSEYLVVGYVSSTFRDAI
                     AFVILILVLLIKPAGILGRNVAEKV"
     gene            complement(9768..10967)
                     /locus_tag="VFM00_13570"
     CDS             complement(9768..10967)
                     /locus_tag="VFM00_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7499321.1"
                     /translation="MLRMRTLSRAVVAALFTAAVVLPLGCAKRSNEIPVGIYGSLTGT
                     TATFGVSTKNGSEMFIDNLNAAGGIAGTKIHAYVEDDRSLPEEAATAVTKLIDQNGVI
                     AVLGEVASSRSMAAAPNCQQAGVPMISPSSTNPKVTELGDYIFRVCFIDPFQGQMIAK
                     FAKNTLNMNRAAVLRDLKNDYSVGLANYFTEAYQALGGTIVIDQSYSEGDQDFKAQLT
                     KIKAKNPQFIMVPGYYTEVGLIARQARELGITAPLLGGDGWVSEKLLEIAEDALNGSY
                     FVNHFYEGDPNPMVQKFVADYQQRYNSKPDGLAALAYDAAGVLSVALQKLHDEDPKSF
                     QALMGPRNDAQKAARAKLRDLIAATKDYPGVTGRITLDAKRNAVKPAVFIGIEKRAYK
                     YVATVEP"
     gene            complement(11088..>12067)
                     /locus_tag="VFM00_13575"
     CDS             complement(11088..>12067)
                     /locus_tag="VFM00_13575"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7499322.1"
                     /translation="GKTFVNGLRIAVEEHNREFAPSISLVLYDSEGDPLVGARKARWL
                     LKDHGVSLLIGELFTANTAPLAAATQVVGAVLLSPSATNERLATLGDAVFQLHVPPAT
                     LASALARYLKSAAPRNTAALLVAQTPDDSISGAQVAAACKDQGVSVVAVERVPDGTVD
                     LTRPLAALRAKKAKTLVILGSPRLVGVAAPQIASAWPDVKVAGFESLDPEGLLREARE
                     ALDGASFFASDYALLGAPADSFAARYQRVNQEAPTRMSTRGYLVGMAVARAIESGCVN
                     AAGLRETLRGQLYDGEEGRVLHALRPVIPAEPERLVVRGGKAIAAEIAP"
BASE COUNT         1722 a         4339 c         4224 g         1782 t
ORIGIN      
        1 gctcttcgta gtcctggacg gcgagcaggt tcacgggacc caggccgcgg agcttttcct
       61 ggagctggcg cagacggtcg cggcgtccct cgggcgaggc caggtccacc acagaggtcg
      121 aactgactgc tgctggcgcc atcggcggct ccgccgcctc ggcggacatg gcaaccgcgc
      181 cctcctcgac gtcggcaacg tcgatctcct cgatctcgac cgccccttcg ccctcgaccg
      241 gaaccgccgg cacgtacgcg gcgagatcca gctcgtactc gttccagatc cgctcgatca
      301 gccggcgcgc ggcgccctcg gattccgtct gctccaactc gatccgatgg agccgctcgg
      361 tcagttcctg gtgctcgcgg cggaagtcgc gcgtgcgggt ctcgatatcg tccacgttcc
      421 ccttcaccgc gccgaagcgc tcgcgcgtgc cgtgcagcac cagctccttc tccgccttga
      481 ggcggatctc ctcggccagc tcttcttccc ggcgggcgca cagggtcgta gcttcctcga
      541 tccggcgctc cgtctcggac gcctcttcgt cgcggctttg gatatcgcgc tccgcctcgt
      601 cggcgctctg acgcatccgc tcgatttcgc gccgggccag gtcgtacgcg gcgcgggagc
      661 gcgcggcctc gatccgggtc tcggcgtgct gcgcggtgag ccggtcccgc tcctgctccg
      721 tggcccggaa ctccgcctct tcctgcgcca gcatgtcgtc cacgcggccg cgctcggcct
      781 cggcgctccg gagcttctcc tcggccgcct cggccgcctc gcgcgtgcgc gccgcctcct
      841 cttccagcga ccggagcgcg tcgaccagtg acttggacgc cttggccatg gcgtcgcccc
      901 ggatcgtctg ctcggcgcgg cgtccttcca gcgcgcgatg cgcctcctcg accgaagcga
      961 gcgcgcgggc cgcgccttcc tgggccgcct gcgcctcggc gcgctcggcg cggagcttct
     1021 ccgaagcgcc gagcgtctcg gccagggccg ccgacgagcg ctcgatcgag gccgccagct
     1081 cgaggatctc gtcctcgcgc cgcagcaccg atcccgcgcg cgtggcgccg cttccgccgt
     1141 ggatgatccc gggggcgacg atccgctccc ccgtctcggt gacgaagtgg agccgctgct
     1201 cggggtgctt caagatcagg tcgaggccgg tgcgcaccga gtccacgacg atgattcccg
     1261 acaacaggaa ggacgcgagc gtgatgtagc ccggttcgaa gcggacgtgc tcgatcaggg
     1321 ggccgcggac gccgggcgcg ctcagcacct cttcggggat gggcgcgacg cgcgcgcgcg
     1381 agatccggtc cagcgcgatg aacgtcgcgc gcccttcgcc gctctcctcc agcgcggtca
     1441 gcgccgcgag cgcggcgtcg gtgcgctcgg ccacgatgaa ctgcaccgcc tcgccgaggg
     1501 cggcctccag cgcgaccagc cattcgcccg aggcgtggag cacgtcgccc accgtgccga
     1561 ggatccggtc ggggcgggcc ttccccgcga ccagccagcg caccgaggcg tcgtagccct
     1621 cgtagctgtt cttcagctcg gtcagcgtgg cgtgccgcga ctccagcgcc gcgtgcgcct
     1681 cgcgcgcgcg cttctccgtc tcgtcctgcg cgaggaggcg ccgctccacc tgggcgatcg
     1741 cctcgaccgc gcgctgaacg ccgcgcttgg cttcctcgac gtcgagcgcg gacgtctcga
     1801 tcgaacgggc gacgtcggcc agcatggcgc ggagcgtctc ctcctcgttc gcgatcgacg
     1861 agagctgggc ggcgagctcg tccttccggc ggctcaggtc ctcttccttg gtccggagcg
     1921 accgcgcgct cgactcctgc tccacgcggg cctggaagag atcgagggag agctgcttcc
     1981 gggtcccgag cgcggcacgg cgctcttcga gcaccgggcc cagctgcgcg agccgcaccg
     2041 ccagcgcctc ttcctcggat cccttctccg cctgcacctg ctccaggcgc acctgctctt
     2101 cggccaggcg caccgcggcg gcgcgcacct cttcgacgct ggcgcggagc cggctggact
     2161 cggagcgcgc gtgctccagc ttctcggcca ggttcgaccg gcgctcgcgc agcaccgcga
     2221 cctcgttcag gagtgccgca ccccgtgcct cgtgctcgct cagcgcctcc tgcgcgagcc
     2281 ggacttctcc ctcgcgcttc aacagctcga gcttcaattc ctccagctcg gcctcgtggc
     2341 gcgccagatg cccggcgcgc tcggcccgcc gctgctccag ggactggcgc tcggcgcgca
     2401 gcccgcccga gcccgacgtc agccggtcgt actcctcctt cgccagcccg agatcgatgg
     2461 aacggatctc gtccttcaga cggccgtact tgcgcgcctt ccccacctgg cgcgccagcg
     2521 agcgcacctc gcgctcgatc tcgcccacca cgtcgttcac gcgcgtcagg tcggtcagcg
     2581 tgagatccag cttcgccagc gcctctttct tccgcgtctt gtagcgcatg atcccggccg
     2641 cttcctcgaa caggaagcgc cggtgaccgc tctcgtcgga gaggacgttg tcgaccatgc
     2701 cgcgctcgat cagcgagtag gcgtgcgagc ccatgccggt gtcgaggaag aggtcgcgga
     2761 cgtcgcgcag gcggcagagg ctcttgttga tgaagtattc gctctgcgcg ttccggaagg
     2821 tgcgccgcgc gatggccagc tcggtgtact ccgaggggag cacgccgcgg ttgttgaaca
     2881 tggtgagcga cacctcggcc atgccgagct gcttccgctg cgccgagccg gtgaagatca
     2941 cgtcctcgag cgtatcgccg cgcaggtggc gcatgttctg ctcgcccagc acccagcgga
     3001 tcgcctcgac gatgttcgtc ttgccgctcc cgttcgggcc gaccaccgcc gtgatcccgg
     3061 ggccgaagtc gacgcggacg cgctgcggga aggatttgaa gccctggatg tcgagtcgct
     3121 tgaggaacac ggcctacctc ttcgtttgcg ggtagctctg cgcgcggtcg accgcgccgc
     3181 tcttgatcag ccactgggcg tacgtggccg cctcggcttc ggtcgcgaac cgccccacga
     3241 acacgcggta ccagtcgccg cccggagggg gcggctcgaa ccaggcgtcc agcttcttcg
     3301 cgcggagggc cgccacgatc tcccgcacct tggtctcggt gcggaaggag gcgacgtgga
     3361 cgcggaacgg cttggcggcg tcgccagcgc cggtgccgat gccggcgagc gagcccgtgc
     3421 ccgtaccgcc caggcccgcg cgaagcgctt ctcgtgccgg gggccgggcc gtcgccgagg
     3481 gcgatgcggc ggacggcgtg gcgggcgcgc ctgccggagg cggcgtgctc gagcccgccg
     3541 cgggcgtctc cgcgctctgg atctccttcc ccattgcggt gaccgatccg atctggccgg
     3601 agcccggcgt cgccggtgcg ccgaccatcg ccgagggatc gtcgagattg ccgcgctcgt
     3661 cgggcgccgg cgccggcgtc accggctcct cgcccgcggg ctggtttgcc tggaccgatc
     3721 cccggggcgg agcgggacgc ttcgagagca gggccgaaag tccgcccgcc acgagcgcca
     3781 gcccgagcac cgctcccgcg atgacgagga ggtttccgcg cttggcggcc ggcttcttct
     3841 cgacgatgcc ccggcgcacg tcgcgctcga gggccgcctc caggtcgcgc tcggcgcccc
     3901 ggtcttccat gcgggtcgcc ctcgctgccg gagcggggga cgccggtgcg gcggcgcggg
     3961 ccacctcggc ggcgtagagc gcggccgagg cctcacgttc ccacgcggga ggctccggag
     4021 acatgggcgc gggcgattcg agagccgggg cgagggccga cacgcgggca tgccccgatg
     4081 ccgcctcggc ccggctcgcc gtgggggcgt cggcgggctc gatccccacg cagccgatca
     4141 tcggcgcgtc ggaggcccac agctcgagga aggcgtcgcg caagtcggcg cgtccgatcc
     4201 gctcggtggc ggcggccagg atcacggcgt ccatcttctt cgagagcttg ccggtgaagc
     4261 cgcggcggtc caggagcggt gcggagtaga tcgccacgtc cacgcaggag gcgaagacgc
     4321 gcgccgcccg cacccagtcg gggtgctcgg cgacgcgcga ctcctcgggc gggtggcttc
     4381 cgggaccgat cagccagagc ttgtcggcca cgatctccca ggcgacgcgc ccgaacgagc
     4441 atccgtattc caggacgtcc accagcccct cgttgggatg gcttggcggg cgcgagacgg
     4501 tcggattcca gaaatcgagg tcgatcacgg cggtccggag accgcggcgg ctccactcgc
     4561 gcgccaggca gagcgcggtc gcgaagcatt cgggatcgtg cgggaagccg gcgaccagga
     4621 gcgagcggag ggtcagcgaa cggcgggtct cgttgaggct gtcgacgacg gggcccagga
     4681 cctcggcgcc ggacgagatg tcgagcagct gggccaagcc agctcccgcg ggcaccgggc
     4741 gcacgcgcgc cgagcccaag agcggcaagt tcacttcgcg agcgaaatcc gcggggttcg
     4801 cgtagcgctc cggataggtc tccaccgccg ccatctaccc ttcctcgtcg aggacccgag
     4861 ccaccaccgc gaccaaccgg ggaacatcag gccccggcac cgtacgcatg tccaggagaa
     4921 cccttcccgc ttcgatacgg gccagcaccg gatcgggccc gaggcgaagc gcgcgagcga
     4981 gcgtttcggc gcggccgcgc ggcgcacgga gcgccagcgc aaaggacggc acgcgcgcga
     5041 gcggcatggc gccgcccccc acttcaccgc tgcaaggtat cacctccgcg ccggtccgcc
     5101 tagcgcgttc cgcgcccagc gcgcgcaaaa cgcgccgggc gcgggcgcgc accgaggctg
     5161 cggactcgcc gagcatccgg agcaccggaa tcgcggcctc gcggcggtcg ggatcgcggt
     5221 agatccggag cgtcgtctcg agcgcggcga gggtcatctt gtccacgcgg agcgcgcgcg
     5281 tcatgggatt ggcccggcac gccgcgatca gcgctttcgt cccggcgagg atccccgcct
     5341 gcggcccgcc gagcagcttg tcgccgctcg cgcaggcgag cggcacgccc tgggccagcg
     5401 cctcggccag ccgcggctct tcgggaagcc cataggggcg gaaatccacg agcgccccgc
     5461 tccccacgtc ctcgagcagc gggaccttct tcttccgggc caggcgcacc aggtcctcgc
     5521 gggtgacctc ctgggagaag ccgacgatgg cgaagttgct cgggtgcacg cggaggatcg
     5581 cgcccgtcgc ggggccgatc gcgcgctcgt agtcggccag gcgcgtcttg ttcgtcgtgc
     5641 ccacctcgcg caggcgggcc cccgcgcgct ccaggatctc cggaatccgg aaggagcccc
     5701 cgatctcgac cagctgcccg cgggagacga ccgcctcgcg gtcgcgcgcc agcgtgttca
     5761 gggcgaggag cattgcggcg gcgttgttgt tcacgacgag ggccgcgggg gcgcccagga
     5821 tctcccggag cagcggctcc aggtgcgcca tgcgcgagcc gcgagagccc gaatccagat
     5881 ccagctcgag gttcgtgtag cggcgcgccg cctcggcggc ggcgagcgcg gcctcctcgg
     5941 ccagcggcgc ccgccccagg ttggtgtgga gcacgacccc ggtggcgttc aggaccgacc
     6001 ggaggctggg gcggaggcgc gcctcggcgg cggcggcggc ctccgccgcc agggcgtgcg
     6061 ccgagggggg cgcgggagcg gcaccccggc ggatctgatc gcgcgcgcgg ccgatcgcct
     6121 cgcggaccgc gccgaccagg agcgcgtggg gcgccttgcc ggcgagcgcc tggagcgggg
     6181 gcaaggagag gagcgactcc accgagggca gcgctcgaag cgcgtcgttc gtggcgccgc
     6241 gcttcggctt cacgccgctt ccctccgcgc ttcgcgctgc ggggacatca cgccgcttcc
     6301 ctccgcgctc cgcgctccgg ggatatcacg ccgcgccccc gagatacgcc tcctgcacga
     6361 aggggttggc gagcagcgcc tccgccttgt cggagaggcg gatcactccc gtctcgagca
     6421 cgtagcccac gtcggccacc gccagcgcca tgcgcgcgtt ctgctcgacc aggacgatcg
     6481 tggtgccgcg ctcgcggatc tcccggatca cgccgaagat ctcgcgcacg aggagggggg
     6541 ccaggcccag gctgggctcg tccatcagga ggatcttcgg gcgggccatc agggcgcggc
     6601 cgatcgcgag catctgctgc tcgccgccgc tcagcgtccc ggaggactgg cgctcccgct
     6661 cttggagtcg cgggaagagc gtgaagacct tgcgccggtc ttcctcgatc cccttccggt
     6721 ccttgcggag ataggcgccc atctccagat tgtcggccac cgagaggttc gcgaacacga
     6781 tccgcccctc ggggacgtgg gagatcccca ggcggacgat cttgtgcgcg gggacgccca
     6841 cgatcgactt cccgtcgagc aggatgctcc cctggctcgg ccggatcagc cccgagatgg
     6901 cgcgcagggt ggtgctcttg cccgcgccgt tggcgccgac cagcgtgacg atctgacccg
     6961 cctcgatccg gatcgagatc cccttgagcg cgcggatccc gccgtagtgg acgtgcagat
     7021 cttccagggc gagcaggctc acgcctcttc ccccaggtaa gcctcaatca ccttgggatc
     7081 tctccggatc gcctccggca gcccctcggc gatcttcacc ccatggtcca gcaccgtgat
     7141 ccgctcgcag atccccatca cgaccctcat gtcgtgctcg atgaggagaa cggtgagccc
     7201 gtagctctcg cggatctcgc ggatcagctt catgagctgg aggctctcct ggggattcat
     7261 gccggccgcg ggctcgtcga ggaggaggag ccggggctcg gccgccaggg cgcgcgcgat
     7321 ctccagccgg cgctgatcgc cgtaggggag gttccgcgct ttctcctcgg cataggcgga
     7381 gagcccgaac gattccagga gctcccgggt ccgcaccgcg atcgccgcct cctcccgctc
     7441 cgagcgcccc gagcgcacga tgctgtcggc cacggtcgcg ccgcagtgga ggtggcaggc
     7501 gatccgcacg ttgtcgtagc aggtcagctc ggcgaagagg cggggattct ggaacgtgcg
     7561 cgacacgccg cgcgcggcca cgcgcgaggt ggcgaggccg tggatcggcg cgccctcgag
     7621 caggatctta cccgacgtcg gggcgtacac gcccgtgatc aggttgaaga cggtggtctt
     7681 ccccgcgccg ttgggtccga tgaggcccgc gagaacgccc ggttcgagcg ccaggtccag
     7741 gtccgagacc gccttcagcc cgccgaacgc gcgggtcacg tggtccagcc ggagcagagg
     7801 ccccgcggtc ccggttccgg cgcgctcctt ccggaacgcg tggcggggcg gcgcgatcac
     7861 ttcgcccccg acccgcccgg ctcgggcgac ggcggcttga accgcccgaa catccgcggc
     7921 agggagaact cgcgatgccc caggatcccc tgcggcctcg cgatcatgag cacgatgagg
     7981 agcgccgaat agatgatgag ccggtcggcc gccgcgaagc gcagcgcctc ggggagtgcc
     8041 gtgagcagca cggctccgag gatcgagccg gtcaggctcc ccatcccacc cagcacgacc
     8101 atcacgatca cctcgatcga cttcatgaag gtgaacgagt tggtgtggag ataggtgtag
     8161 tgggagaaga gggcgcccgc ggcgcccgcg aagaaggccg agatgacgaa tgcggtcacc
     8221 ttgtagcgcg tggtgggcac gcccagcgcc tcggcggcga tctcgtcctc gcgaatggcg
     8281 aggagcgcgc gcccgtgata ggaccgcagc aggttctgga tcacgacgaa ggtcaggatg
     8341 gccccggcca gcacccagga cagcgtcgtg atctgcggga ttcccgcgaa gccgcgcgcg
     8401 ccgcctacgg cgtcgatgtt caggatcgcg acccggatga tctcgccgaa gcccagcgtg
     8461 acgatcgcca ggtagtcccc gcggagccgc atcgagggga gcccgaccag gaaccccgcg
     8521 gccgccgcga tcaggccgcc gatcaggatg gccacgatga agaagagccc cgccacgagc
     8581 ccctgcggcg cattcccccc cagaagctgc ggcaccgcgt agaccgtgaa ggccgcggag
     8641 gtgtaggcgc ccacggccat gaacccggca tggccgatcg agaactgtcc cgtgacgccg
     8701 ttgatcaggt tcaggctgag cgccagcatc acgttcagcc cggagaggac gaggatgcgg
     8761 tagaagtagg gattgatcac gccggagagg aaccggtcca ggagccagac gaccacgagg
     8821 accgccgcgc cggtcaggag gcggcggagc acgctagacc ttctccgcga cgttccgccc
     8881 caggatcccg gccggcttga tcaggaggac gaggatcagg atgacgaagg cgatcgcgtc
     8941 ccggaaggtg gaggagacgt agccgaccac gaggtattcc gagaccccca tcacgacgcc
     9001 gccgatcacc gcgcccggca cgctcccgat cccccccagc accgccgcca cgaacgcctt
     9061 cacccccggc atgatcccca tcaggggctc gatcttggga ttcgtgagcc cgaccagcac
     9121 gccggcggcg gccgcgagcg ccgatccgat cgcgaacgtg atcgtgatga tgcggttcac
     9181 gggaatgccc atgagggagg ccgcgtcgcg gtggaacgcc acggcgcgca tcgcctttcc
     9241 ggtcctggtg cgggcgatga agagggtcag ccccaccatc agcaccaggg agacgacgac
     9301 cacggtgatc tggtggttcg tcaccatgac cccgccgccg aggtcgatgt tcttcggcgc
     9361 gatgagctgc gggaagaact tgggatcggc tccgaagatg cggatgccga ggttttccag
     9421 gagcagcgag acgccgatcg ccgtgatcag ggccgtgagc cgcgaggagc ggcgcaccgg
     9481 ccggtaggca aagaattcga tcagaacgcc gagcacggca caggcggcca tcgcggccag
     9541 gaggaccacc accgcgtagg ccggcgacgg cgtggcgccg ccggcaccca gagcgcgcgc
     9601 ggcgaagtag gcggtgaacg cgcccaccat gtagatgtcg ccatgcgcga agttgatgag
     9661 gcggaggatg ccgtacacca tggtgtagcc gagcgcgatg agggcgtaga tgctgcccca
     9721 cgcgatcccg ttcgcgagct gctggaagag ctcctgcacg cccccgccta gggctcgacc
     9781 gtggcgacgt acttgtaggc gcgcttctcg atcccgatga agaccgcggg cttcaccgcg
     9841 ttccgcttgg cgtccagggt gatccgtccg gtcacgccgg gatagtcctt ggtcgcggcg
     9901 atcagatcgc ggagcttggc gcgcgcggcc ttctgcgcgt cgttgcgcgg ccccatcagg
     9961 gcctggaacg acttgggatc ctcgtcgtgc agcttctgga gcgcgacgct cagcacgccc
    10021 gcggcgtcat aggccagcgc ggcgagaccg tcgggcttgg aattgtagcg ctgctggtag
    10081 tcggccacga acttctggac catcgggttg ggatcccctt cgtagaagtg gttcacgaag
    10141 taggagccgt tcagcgcgtc ctcggcgatc tcgagcagct tttcggagac ccagccgtcg
    10201 ccgcccaaaa gcggcgcggt gatccccagc tcgcgcgcct ggcgcgcgat cagcccgacc
    10261 tcggtgtagt agccggggac catgatgaac tgcggattct tcgccttgat cttggtgagc
    10321 tgcgccttga agtcctgatc cccttcggaa taggactggt cgatcacgat cgtgccgccc
    10381 agcgcctggt acgcctcggt gaagtagttc gcgagcccca cgctgtagtc gttcttcaag
    10441 tcccgcagca ccgcagcccg gttcatgttc agcgtgttct tcgcgaactt cgcgatcatc
    10501 tggccctgga aggggtcgat gaagcagacg cggaagatgt agtcccccag ctcggtcacc
    10561 ttgggattcg tcgaggaggg ggagatcatc gggacgccgg cctgctgaca gttcggagcc
    10621 gccgccatgc tccggctgga agccacctcg ccgagcaccg cgatcacgcc gttctggtcg
    10681 atcagcttcg tgacggccgt ggcggcctct tcgggaagcg atcggtcgtc ctcgacgtag
    10741 gcgtggatct tcgtgccggc gatcccgccc gcggcgttca ggttgtcgat gaacatctcg
    10801 gagccgttct tggtcgagac gccgaaggtg gccgtggtgc ccgtgagcga tccatagatg
    10861 cccaccggga tctcgttcga gcgtttggcg cagcccagcg gcagcacgac cgccgccgta
    10921 aacagcgcgg cgacgacggc gcgactcagc gtccgcatcc ggagcatcaa gcgaccctcc
    10981 atggcgtaag tgcatggaac cgcaatgttt caagacacgg cgccacccta tcgaaacatg
    11041 ggccgcactg caaggggctc ccctcgcccg cgcccgtctc cggcggtcta gggcgcgatc
    11101 tcggcggcga tcgccttgcc gccgcgaacg acgagccgct cgggctcggc cgggatcacc
    11161 ggccggagcg cgtgcagcac gcggccctcc tccccgtcgt agagctgccc gcgcagggtt
    11221 tcccggaggc ccgcggcgtt cacgcatccc gattcgatgg cgcgcgccac ggccatgccc
    11281 acgaggtagc cccgcgtgga catccgggtc ggcgcctcct ggttcacccg ctggtagcgc
    11341 gcggcgaagg agtcggccgg cgctccgagg agcgcatagt ccgaggcgaa gaaggaagcg
    11401 ccgtccagcg cttccctggc ctcgcgcagg agcccctcgg gatcgagcga ctcgaagccg
    11461 gcgaccttca cgtcgggcca cgccgaggcg atctgcggcg cggccacgcc cacgaggcgc
    11521 gggcttccca ggatcacgag ggtcttcgcc ttcttcgccc tgagcgcggc gagcgggcgc
    11581 gtcaggtcca ccgtcccgtc gggcacccgc tccacggcga cgaccgagac cccctggtcc
    11641 ttgcaggcgg ccgccacctg cgcgccggag atcgagtcgt cgggcgtctg cgccaccaaa
    11701 agcgccgccg tgttccgcgg cgccgccgac ttgagatagc gggcgagcgc gctcgccagc
    11761 gtggcgggag gcacgtggag ctggaacacc gcgtcgccca gggtggccag ccgctcgttc
    11821 gtggccgagg gggagaggag cacggcgccc acgacctgcg tcgccgcggc cagcggcgcg
    11881 gtgttggccg tgaagagctc cccgatcagg agcgagaccc cgtggtcctt gaggagccag
    11941 cgcgccttgc gcgctccgac gagcggatcc ccctccgagt cgtagagcac gagggagatc
    12001 gacggcgcga actcccggtt gtgctcctcg acggcgatcc ggaggccgtt cacgaaggtc
    12061 ttcccgt
//
LOCUS       DASSLZ010000359        12257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1769539, whole genome shotgun sequence.
ACCESSION   DASSLZ010000359 DASSLZ010000000
VERSION     DASSLZ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12257
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769539"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFM00_13580"
     CDS             <1..625
                     /locus_tag="VFM00_13580"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499323.1"
                     /translation="VVSRMRETAAEGVLDGEALRAPPVAAVPAWGPSSTYLHPGHVRV
                     RLTPDGLATVGPTAFASNFIGALARIEAPEAGMEFRQGEPAWTLVSGRNRRLTQAMPL
                     DGTVVEVNREAADRRGGTVPKAADWILKIRPARLAENVQNLIRESLTDAWEEAAALRM
                     NAVLAPVGRVANDGGVWVENFGDALSDADWLEIRQEVFPPQQAVILH"
     gene            670..1593
                     /locus_tag="VFM00_13585"
     CDS             670..1593
                     /locus_tag="VFM00_13585"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7499324.1"
                     /translation="MNRRGFLKTLGLAGATVVCPRTSGAAEPSGGAGEPYGVLVDTTR
                     CVGCNTCTSACAEANGLPEPEDPEAVTRPTTTQWTAIGRYKTSKGEVFAKHQCMHCVS
                     PACHSACLTAAMEKTARGPVVWREDKCMGCRFCMISCPFDGPKFEYSSTNPRIRKCVM
                     CAERIAKGQQPACVDNCPEGALTFGKRNDLLREAHKRIGNAPDQYVDHVYGEREAGGT
                     SWLYLASVGFEEIGFPANVGESSYPMLTAPFLYSVPFVLTLAPTLLTSVARAVRKGEE
                     PHDGEASNPAGGNGSAPAPAGSPGFEEEDAS"
     gene            1590..2861
                     /gene="nrfD"
                     /locus_tag="VFM00_13590"
     CDS             1590..2861
                     /gene="nrfD"
                     /locus_tag="VFM00_13590"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7499325.1"
                     /translation="MNGAQSFSEGRPRGGRSLWRFLLSELKPKGPLFTVFNLVSVPVM
                     IAAAVILFVRFTHGLSAVTNLSQEVPWGLWKGFNVVTGVAFAGGAYFVTLLVYVLGQK
                     KYHSIVRITVLNGFLAYVFYAGALMLDLGRPWKMLNVVIGNSFGVSSVLFLIAWHFML
                     YMMAAFLEFSPAIAEWLGMKRLRRALSALTLGAVVFGITLSILHQSGLGALFLMAKGK
                     VHPLWYSEFLPIYFLVSSVFAGLSVVIFEGTLSRRMFAWRMSEQHAAEHDGIVLSLSK
                     ICAAVMYAYVFLKVLDVVHGHKLALLGTSMGHWFLLEVVGFVLVPLAFFTEGVRWKSV
                     AACKVGAVLTMIGIVLNRLNVSIIGFEWMQPVRYIPTPYEVVVTLAVILAEIWVLRWV
                     IVRMPVLNDSPAWVREPVRAAEVPEAAPASV"
     gene            2874..3554
                     /locus_tag="VFM00_13595"
     CDS             2874..3554
                     /locus_tag="VFM00_13595"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HET7499326.1"
                     /translation="METLVQDPFASKGVEYLICLAFLGSLPAYWRHLNRPARLGMETA
                     EEPVLSGWFHLPARAAFHAGHTWAAPAGPGRFRVGLDDFAQKLLGSPGAVALPAVGSR
                     LQRGDRGFAIDVSGSRFDLPAPLAGRVVARNEAALRDPSLLGDPYGAGWLLEVRPSRW
                     GSGLRSLMQGRSAREWLGRAEEALRLRMSPEVGAVLQDGGVPVPGIARALDEDGWEKL
                     ARELLARG"
     gene            3578..4327
                     /locus_tag="VFM00_13600"
     CDS             3578..4327
                     /locus_tag="VFM00_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HET7499327.1"
                     /translation="MDRRTEDPGTYRVFPDHAEPCVWMLAGLLSYRLCDRDFDCEHCP
                     LDAAIHGVHSAPALDAGAVPEGPPLDWGIRDGLTYHPQYGWVSELESGRLRWGIDGAT
                     ARLLDHATEIVMPAAGSDLEQGKVACWVMDDGDVVPLRAPVSGKITRANPALAREPAL
                     LIHEPYGAGWLMEVEAPGGLAMQHGLCGGTERRLRAARQMRRLHRDAMAHLHLDPGVG
                     PTAQDGGERVLDLRRALGRERYHRLVHFILR"
     gene            4436..6130
                     /locus_tag="VFM00_13605"
     CDS             4436..6130
                     /locus_tag="VFM00_13605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7499328.1"
                     /translation="MSVSTLETRSRWIRRAKAVSSSLSLRLFLSLIAVILAAFAVQTW
                     ASVRLASRQYERALYEGAVRFGDLIEQSTQHGMLVNRKEEVHRVIQAIAQAPDMQGVR
                     IYDKNGSIVFSTDSTEIGRVVDLRAEACVTCHGQGTPLHAVPDRARVRIFQNHRGRVM
                     GVVNAIENRAECARAGCHVAPSQQSILGVLDVRMSLAEPDARLASARRRAIVGAVVVA
                     LLAGLVSALFIVLMVRRPVQRLIRGARLVASGDLSGEIPVEGRTEMAELAGAFNEMTR
                     ELALARRELTDWSGRLESRLQERTADLTRAQREVAHMDKTASLGRLAATVAHELNNPL
                     GGILNYAKLTSRTLRESGVDEAVRRETDGYLGLIQKEADRCGVIVRSLLTFARRSGAP
                     MGPHALRPILERALMLVRHHFEISQIRVEAPPFDGDDALVCDADQIHQALVALFVNAV
                     EAMPQGGSLRITTTGDADQLRVSVADTGVGIPPDALEHIFEPFFSTKDRQEGVGLGLA
                     VVHGIVQRHGGRIEVESEVHRGTTFHLILPRRPSGPGDHHAGDGARSADQPALSGV"
     gene            6134..7507
                     /locus_tag="VFM00_13610"
     CDS             6134..7507
                     /locus_tag="VFM00_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7499329.1"
                     /translation="MAVREQDVVAVSPDLGSVLIVDDEFSMRDSLVHWFEKDGYRTGS
                     AQDGREALQRLDEQDWDAVLLDIKMPGMDGLELQRRIREVDPDLPIIMITAFASVDSA
                     VQALKEGAFDYVTKPIDPDELSHLVRRAVEKRRLQRENAQLKRNVDQLSTPTTIVGAS
                     GPMSKVLELVASVAQTDATVLIRGESGTGKELIAQTIHANSRRRYFPIVPVNCGAIPE
                     SLLESELFGHEKGAFTGAQYRRKGKLEMADGGSLFLDEVGTISLPMQVNLLRVLETKE
                     FTRLGGTKPVRVDFRVICATNQDLEKQVKEGTFREDLYFRLNVVTISLPPLRERRQDI
                     PLLAQHLLGKYATEMNRPFTGFDPAAMDLMVRYEWPGNVRELANAVERALVVGRGPLV
                     AAEDLPMRLNEKTGPPAGDSLVDVEKAQVKAILERTGGNVTRSAEILGIDRVTLYNKI
                     KKYGLRP"
     gene            7518..8093
                     /locus_tag="VFM00_13615"
     CDS             7518..8093
                     /locus_tag="VFM00_13615"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HET7499330.1"
                     /translation="MPGPGIDVVPIYFTERDETLQRLGAALELDFRTTVRYRNPWFDP
                     EAAFDAGRGQYRAALFLKALLLDSQADPAERVIGVTSVDLFTPVLTYVFGEAQLGGRA
                     AVVSCHRLRQEAYGLPPDPRLFYDRLHKEAVHELGHTFGLMHCRSIGCVMGASTYAEE
                     IELKAASFCERCRAVVARNGAPVLSSPHGPR"
     gene            8080..8484
                     /locus_tag="VFM00_13620"
     CDS             8080..8484
                     /locus_tag="VFM00_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HET7499331.1"
                     /translation="MALDDAARAEDELLQLSRRLLDVIASGDWDAYVAICDPSLTCFE
                     PEARGERVTGLDFHHFYFRERRPAEAAQTTLIEPEVRMLGPDAAAVTYVRLTQSRDAA
                     GAACTSRAEETRIWQRRDGRWKLVHVHRSMNP"
     gene            8481..10955
                     /locus_tag="VFM00_13625"
     CDS             8481..10955
                     /locus_tag="VFM00_13625"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7499332.1"
                     /translation="MTAKELSARLTAKEHSARLTAKEPSAALLRAGAIAFAVALLASL
                     AVPSFPLARAARIPASALLVALLFVHGGPGGRARFRVLAWGALVFVAGWGSTGRGAPA
                     IGALIAAILLALFVRRPLAPWMETRPTGWLRLFALGAFLPLAIVLAAGSGAAAHDNAF
                     IRLDRALYTLAVSWIFLSARLSVGELLHRWIRRARIRTKLLVAFGIFAVTPALLAFTY
                     ATLAGWIHAGSLRASVIERQLGARSGGRGLIARAREGAPPASGAELAARIERERPVLV
                     DRGLAAVALERGPEGWRTIASYGDPDSLFRPAAAPVADSGDVVQGLVYRNRRFWWAET
                     ALWPKGGDTLALLTYEPIDTTRVSRLARDMGCDAVLMTSTTMFSGGTKVEVGPKRRNQ
                     RHFRMAGGIVEIGTDEPLPDGVSDSVALDSLSSLGGLSLVGGGDYATATSLNQIRKTS
                     NGGSTPLCYLWRGGEWHRGAALLLVHSPPGESFQYSGMDLGPIAVAARVALIVFAIIF
                     ISLEIVSLVVGARVARFITRGAAGLRQAAVSIGQGDFSARVRVPSEDELGELADSFNR
                     MAAGLEEGQRAMVEREQMRRELELARRIQSRLLPPGPPSLPRLDVAAANAMSQQVGGD
                     YYDFISLENGHVGFCIADVAGKGVAAALLMSSVKTALVSSAAVETAPHLLTGRVNRLL
                     EQSIEPGRFVTFFLASLDPSTMRLEYVNAGHPAPMLLRAAGGVERLEMGGIILGIDAA
                     AKFEAGAVTLAPGDLLAMFTDGVTEAQGADEELFGDARIESVLHLNRDRTAEEVLNAL
                     IEAVKSYEGARGPSDDLTAVVVKLER"
     gene            complement(11056..12111)
                     /locus_tag="VFM00_13630"
     CDS             complement(11056..12111)
                     /locus_tag="VFM00_13630"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7499333.1"
                     /translation="MDAARTFSMRDAARILHVTEPRVRTWARMGGIVPKDGPGGPLEF
                     TFQELLLLRTTRRLLDAGVPASRVKRIWSTLKQQLSEEHPLSSIRIRADGDRAIAWDG
                     KAPWQPESGQFVLDFVAGDVAGASEAPLFEVMSAGGGAEEEGVEDVAAAPQEWPGKPS
                     AHLQKSAQTPADPLQKSSRTPAAPLQKSSGAPAVISIFRDVASKRATERREAPPDESA
                     EHWFRLGSEMESTSPLEARQAYLKALDADPNYADAHLNLGRLDHRAGDLAAAEARYRR
                     AIQCDPEDATSHFNLGVLLEDRNCPQAAAEAYEQAIALDPDLADAHYNLGLLLEKLGQ
                     RADALRHLMAAHRLYAR"
BASE COUNT         1752 a         4134 c         4398 g         1973 t
ORIGIN      
        1 ggtcgtctcg aggatgcgcg agacggcggc ggagggagtt ctggacggtg aggcgcttcg
       61 cgctcctccc gtcgcggcgg ttcctgcctg gggaccgtca tcgacgtatc tccacccggg
      121 ccatgttcgc gtgcggctga cgccggacgg cctggccacc gtggggccga ccgcgttcgc
      181 gtcgaatttc atcggcgcgc tggcgcggat cgaggccccg gaagccggga tggagttccg
      241 acagggcgag ccggcgtgga cgctggtctc ggggaggaac cggcggctca cccaggcgat
      301 gccgctggac ggcaccgtcg tcgaggtgaa tcgcgaggcc gccgatcggc gcggcggaac
      361 cgtgccgaaa gcggccgact ggatcctcaa gatccgcccc gcgcggcttg cggagaacgt
      421 ccagaacctg attcgcgagt ccctgacgga cgcatgggaa gaagcggcgg cgctccggat
      481 gaacgccgtg ctcgcgccgg tcgggcgcgt cgccaacgac ggcggcgtgt gggtcgagaa
      541 cttcggcgac gcgctgagcg acgccgactg gctcgagatc cggcaggagg tcttcccgcc
      601 gcagcaggcc gtgattcttc actgacgacg cacgggaacg tggcgcgcgc atcgaggatc
      661 ggggggcgta tgaaccgcag gggttttctc aagacgctcg gcctggctgg agccacggtc
      721 gtgtgtccga ggacgtcggg ggcggcggag ccctcaggcg gcgccggtga gccgtacggc
      781 gtcctggtgg acacgacgcg ctgcgtgggg tgcaacacct gcacctcggc gtgcgcggag
      841 gcgaacgggc tcccggaacc ggaggatccc gaagccgtga cccggccgac cacgacgcag
      901 tggacggcca tcggacgcta caagacgtcc aagggcgagg tgttcgcgaa gcatcagtgc
      961 atgcactgcg tctccccggc gtgccattcc gcctgcctca cggccgccat ggagaagacg
     1021 gcccggggcc cggtggtctg gcgcgaggac aagtgcatgg gctgccgctt ctgcatgatc
     1081 tcctgcccgt tcgacgggcc gaagttcgag tactcgagca ccaacccgcg catccggaag
     1141 tgcgtgatgt gcgcggagcg gatcgcgaag ggtcagcagc cggcgtgcgt ggacaactgc
     1201 ccggaagggg cgctcacgtt cgggaaacgg aacgacctgc tgcgcgaggc acacaaacgg
     1261 atcggcaacg cgcccgacca gtacgtggac catgtctacg gcgaacgcga ggcgggcggc
     1321 acgtcgtggc tctacctggc ctccgttggg ttcgaggaga tcgggttccc agccaacgtg
     1381 ggcgagtcgt cctatccgat gctgaccgct ccgttcctct acagcgtgcc cttcgtcctg
     1441 accctggccc cgacgctcct gacgagcgtc gcgcgcgcgg tgcggaaggg tgaggagccg
     1501 cacgacggcg aagcttcgaa ccccgcgggc ggcaacggtt cggcgccggc ccccgctgga
     1561 tcccccggat tcgaagagga ggacgcgtca tgaacggcgc ccaatcgttt tccgagggcc
     1621 gcccccgcgg cggacgaagc ctatggcggt tcctgctgtc ggagctgaag ccgaaggggc
     1681 cgctgttcac ggtgttcaac ctggtctcgg tgccggtgat gatcgccgcg gccgtgatcc
     1741 tcttcgtgcg gttcacccac ggcctttcgg cggtgacgaa cctgtcgcag gaagtgccct
     1801 ggggcctgtg gaagggcttc aacgtggtca ccggcgtcgc cttcgcggga ggcgcctact
     1861 tcgtgacgct cctggtctac gtgctgggac agaagaagta ccactcgatc gtccggatca
     1921 cggtgctgaa cgggttcctg gcgtacgtgt tctacgcggg cgccctcatg ctggacctcg
     1981 gccggccgtg gaagatgctg aacgtggtca tcggcaactc gttcggcgtg agctcggtgc
     2041 tcttcctgat cgcctggcac ttcatgctct acatgatggc cgcgttcctg gaattctcgc
     2101 cggcgatcgc ggaatggctg gggatgaagc ggctgcgccg cgcgctgtcg gcgctcaccc
     2161 tgggcgccgt ggtcttcggg atcacgctgt cgattctgca ccagtcgggg ctcggggcgc
     2221 tgttcctcat ggcgaaggga aaggtccatc cgctctggta ctcggagttc ctcccgatct
     2281 atttcctggt ctcgagcgtc ttcgcgggcc tctccgtcgt gatcttcgag ggaacgctca
     2341 gccgccggat gttcgcgtgg cggatgagcg agcagcacgc cgccgagcac gacggcatcg
     2401 tcctgtcgtt gtccaagatc tgcgccgcgg tgatgtacgc ctacgtgttc ctcaaggtgc
     2461 tggacgtcgt ccacggccac aagctggcgc tcctgggaac gtcgatgggg cactggttcc
     2521 tgctcgaggt ggtgggcttc gtcctggtcc cgctggcgtt cttcaccgag ggcgtgcgct
     2581 ggaagagcgt ggcggcctgc aaggtcggcg ccgtgctcac gatgatcggc atcgttctga
     2641 atcgtctgaa cgtctcgatc atcgggttcg agtggatgca gcccgttcgc tacatcccca
     2701 cgccctacga ggtggtggtg acgctcgcgg tcattctcgc ggagatctgg gtgctccgct
     2761 gggtcatcgt ccggatgccg gtgctgaacg actcgccggc ctgggttcgc gagccggtgc
     2821 gcgcggccga ggtccccgaa gccgcgccgg cgtcggtgta ggcggaggaa gtcatggaaa
     2881 cgctcgtgca ggatccgttc gcatcgaagg gtgtcgagta tctgatctgc ctcgccttcc
     2941 tggggtcgct gccggcctac tggcgccatc tgaaccggcc ggcccggctg gggatggaga
     3001 cggcggagga gcccgtcctg agcggctggt tccatctgcc cgcgcgggcc gcgttccacg
     3061 ccggccacac ctgggccgcg cccgcggggc cgggacgctt ccgcgtcggc ctggacgact
     3121 tcgcgcagaa gctcctgggc agccccggcg cggtggcgct cccggcggtc ggatcgcgcc
     3181 tgcagcgcgg agaccggggg ttcgcgatcg acgtcagcgg gagccgcttc gatctgccag
     3241 cgccgttggc gggacgcgtg gtcgcgcgca acgaagccgc gctccgcgat ccctcgctcc
     3301 tgggcgatcc ctacggagcc gggtggctcc tggaagtgcg gccgtcgcgc tggggatcgg
     3361 gactccggtc cctgatgcag ggaaggagcg cgcgggaatg gctgggtcgc gccgaggagg
     3421 cgctccgcct gcggatgagt cccgaggtgg gcgcggtgct gcaggacggc ggcgttccgg
     3481 tgcccggcat cgcgcgtgcg ctggacgaag atggatggga gaaactggcc agggagctgc
     3541 tcgcccgcgg gtgagctccc tgaagccgag gtgcgacatg gatcggcgaa ccgaagatcc
     3601 cggaacctac cgagtgtttc ccgatcacgc cgagccctgc gtgtggatgc tggcggggct
     3661 cctcagctat cgcctctgcg accgcgattt cgactgcgag cattgtcccc tggacgccgc
     3721 gatccatggc gtccactccg cgccggctct ggacgccggc gccgtgccgg aggggccgcc
     3781 gctggattgg gggatccgcg acggcctcac ctatcacccg cagtacggct gggtctcgga
     3841 gctcgagtcc ggccgcctcc gatgggggat cgacggcgcc acggcgcggc tcctcgatca
     3901 cgcgaccgag atcgtcatgc cggcggccgg gtccgatctg gagcagggga aggtggcctg
     3961 ctgggtgatg gacgacggcg atgtcgtccc gctccgcgca ccggtgtcgg ggaagatcac
     4021 gcgcgccaat cccgcgctcg cgcgcgagcc ggcgcttctg atccacgagc cctacggcgc
     4081 aggctggctg atggaagtgg aggctccggg cgggctcgcc atgcagcacg gactgtgtgg
     4141 cggaaccgag cggcgccttc gcgccgcccg ccagatgcgc cggctccatc gcgacgccat
     4201 ggcccacctg cacctggacc ccggcgtcgg gccgacggcc caggacggcg gcgagcgcgt
     4261 cttggacctg cgccgggccc tgggccggga gcggtatcac cgacttgtcc atttcattct
     4321 tcgataatca cttgagcccg tgcggagggt gcccggcaag gtgggacctt tggcccatat
     4381 cggtcggggt ccgcacgggc cgtagaatga ttcaacaggc cagacgcggc ggggggtgag
     4441 cgtgtccacg ctggaaacaa ggtcccgctg gatccgacgg gcgaaggccg tttcctcgtc
     4501 cctgagcctc cgactattcc tttccctcat cgccgtcatc ctcgcggcat tcgccgtcca
     4561 gacctgggcc agcgtccgcc tcgcctcgcg ccagtacgag cgcgcactct acgaaggggc
     4621 ggtccggttc ggcgacctca tcgagcagtc cacccagcac gggatgctgg tgaaccgcaa
     4681 ggaagaggtc caccgggtga tccaggccat cgcccaggcc cccgacatgc agggggttcg
     4741 catctacgac aagaacggct cgatcgtctt ctcgaccgac tccaccgaga tcggcagggt
     4801 cgtggacctc cgggccgagg cctgcgtcac ctgccatggg cagggcaccc cgctccacgc
     4861 cgttcccgac cgcgcgcgcg tgcgcatctt ccagaaccat cgcggccggg tcatgggcgt
     4921 cgtcaacgcc atcgagaatc gcgccgagtg cgcgcgcgcc ggctgtcacg tcgctccctc
     4981 gcagcagagc attctcggcg tgctggacgt gcgcatgtcg ctggccgagc cggacgcgcg
     5041 gctcgcctcc gcgcgccgcc gcgccatcgt cggcgcggtc gtcgtggccc tgctggccgg
     5101 gctggtgtcg gccctcttca tcgtgctcat ggtgcgccgg ccggtgcagc ggctgatccg
     5161 cggcgcgcgg ctcgtcgcct ccggcgacct gagcggcgag atccccgtcg agggtaggac
     5221 ggagatggcg gagctggccg gcgcgttcaa cgagatgacg cgggagctgg cgctggcccg
     5281 tcgcgagctg accgactggt cggggcgcct cgagtcgcgg cttcaggagc gcaccgcgga
     5341 tctgacgcgc gcgcagcgtg aggtggcgca catggacaag acggcgtcct tggggcgcct
     5401 ggccgccacc gtcgcgcacg agctgaacaa ccccctcggg ggaatcctga actacgccaa
     5461 gctgacctcg cggacgctgc gggagagcgg cgtcgacgag gcggtccgcc gcgagacgga
     5521 cgggtatctg ggcctgatcc agaaggaggc cgaccgctgc ggggtcatcg tccgcagcct
     5581 gctcacgttc gcgcgccgga gcggcgcgcc gatggggccg cacgcgctgc gtcccatcct
     5641 ggagcgcgcg ctgatgctgg tccggcacca tttcgagatc tcccagatcc gggtggaggc
     5701 gccgccgttc gacggagacg acgcgctcgt ctgcgacgcg gaccagatcc accaggccct
     5761 ggtcgcgctc ttcgtgaatg ccgtcgaggc gatgccgcag ggtggctcgc tgcggatcac
     5821 cacgactggg gacgcggacc agctgcgcgt ttcggtcgcc gacacgggcg tggggatccc
     5881 gcccgacgcg ctggagcaca tcttcgaacc cttcttttcc acgaaggacc ggcaggaggg
     5941 tgtcggcctc ggtctcgccg tggtgcacgg catcgtgcag cgccacggcg gccggatcga
     6001 ggtcgagtcc gaggtccacc gcggaaccac gttccacctg atcctcccgc gccggccctc
     6061 cggaccggga gaccaccacg cgggcgacgg agcccggagc gcggaccaac ccgcgctgag
     6121 cggagtctag gtcatggccg tgcgcgagca ggacgtcgtc gccgtttcgc cggacctggg
     6181 aagcgtcctc atcgtggacg acgagttctc gatgcgggac tccctggtcc actggttcga
     6241 gaaggacggt taccggacgg gcagcgcgca ggacggacgc gaggcgctgc agcgcctgga
     6301 cgagcaggat tgggacgcgg tgctgctcga catcaagatg cccggcatgg acggcctgga
     6361 gctgcagcgg cggatccgcg aggtggaccc ggatcttccg atcatcatga tcacggcctt
     6421 cgcgtcggtg gactccgcgg ttcaggccct caaagagggg gcgttcgact acgtcacgaa
     6481 gccgatcgat cccgacgaac tgagccatct ggtgcggcgc gcggtggaga agcggcgcct
     6541 gcagcgcgag aacgcgcagc tcaagcgcaa cgtggaccag ctctcgacgc cgacgacgat
     6601 cgtcggcgcg agcgggccga tgtcgaaggt gctggagctg gtggcctcgg tggcccagac
     6661 ggacgccacc gtgctgatcc gcggggagag cggcaccggc aaggagctga tcgcgcagac
     6721 gatccacgcg aacagccggc ggcggtattt tccgatcgtt ccggtgaact gcggcgccat
     6781 ccccgagtcg ttgctggagt cggagctgtt cggccacgag aagggcgcct tcaccggcgc
     6841 ccagtatcgc cgcaaaggga agctggagat ggccgacggc ggcagcctct tcctcgacga
     6901 ggtggggacg atcagcctcc cgatgcaggt gaacctgctg cgcgtcctcg agaccaagga
     6961 gttcacccgg ctcggcggaa caaagccggt gcgcgtggac ttccgcgtga tctgcgcgac
     7021 gaaccaggat ctggagaagc aggtgaagga ggggaccttc cgcgaggacc tctacttccg
     7081 cctgaacgtg gtcacgatct ctcttccgcc cctgcgcgag cgccggcagg acatcccgct
     7141 cctggcccag cacctgctcg gcaagtacgc caccgagatg aaccggccgt tcaccggatt
     7201 cgatcccgcc gcgatggatc tcatggtccg ctacgaatgg ccggggaacg tgcgcgagct
     7261 ggccaacgcg gtggagcgcg cgctggtggt ggggcgcggg ccgctggtgg cggccgagga
     7321 cctgccgatg aggctcaacg agaagaccgg cccgccggcg ggggactccc tggtcgacgt
     7381 ggagaaggcc caggtcaagg cgatcctcga gcggacgggc gggaacgtga cgcggtcggc
     7441 cgaaatcctg ggcatcgacc gggtcacgct gtacaacaag atcaagaagt acggccttcg
     7501 cccgtaacgg cgccgccatg cccggccccg gtatcgacgt cgtgccgatc tacttcaccg
     7561 agcgggacga gacgctgcaa cgtctgggcg ccgccctcga gctggatttc cgcacgaccg
     7621 tgcgctaccg gaatccgtgg ttcgatccgg aggccgcgtt cgacgccggg cgggggcagt
     7681 accgcgcggc gctcttcctg aaggcgcttc ttctggactc gcaggccgat ccggccgagc
     7741 gcgtgatcgg cgtgaccagc gtggatctct tcacgcccgt gctgacctat gtgttcggcg
     7801 aggcgcagct gggcgggcgc gccgccgtgg tctcctgcca caggctgcgc caggaggcgt
     7861 acgggctgcc tcccgatccg cggctcttct acgaccggtt gcacaaggag gccgtgcacg
     7921 agctggggca cacgttcggg ctcatgcatt gccgctcgat cgggtgtgtc atgggcgcct
     7981 cgacgtacgc cgaggagatc gagctgaagg cggcttcctt ctgcgagcgc tgccgcgcgg
     8041 tggtcgcccg gaacggcgct ccggtgttat cttccccgca tggccctcga tgacgccgcc
     8101 cgcgcggagg acgagcttct ccagctgagc cgccgcctcc tcgacgtgat cgcctctggc
     8161 gactgggacg cctacgtcgc gatctgcgac ccctccctga cctgcttcga gccggaagcg
     8221 cgcggcgagc gggtcacggg actggacttc catcacttct acttccgcga gcgccggccg
     8281 gccgaagcgg cgcagaccac gttgatcgaa ccggaggtcc gcatgctggg acccgacgcg
     8341 gccgccgtca cgtacgtccg cttgacccag tcccgcgacg ccgcgggcgc ggcgtgcacc
     8401 tcccgagccg aggaaaccag gatctggcag cggcgcgacg ggcgatggaa gctcgtccac
     8461 gtgcaccgct ccatgaatcc gtgacggcga aggagctctc ggcgaggctg acggcgaagg
     8521 agcactcggc caggctgacg gcgaaggagc cctccgccgc gctcctgcgc gccggcgcca
     8581 tcgccttcgc ggtcgccctt ctcgcctcgc tcgccgttcc ttcctttccc ctcgcccgcg
     8641 ccgcgcgaat tccggcgtcg gctctgctcg tagcgctcct cttcgttcac ggcgggcccg
     8701 ggggacgcgc acggttccgg gtcctggcgt ggggagcgct cgtcttcgtg gcgggatggg
     8761 ggtccaccgg ccggggggcg cccgcgatcg gcgccctcat cgcggcgatc ctgctcgcgc
     8821 tcttcgtccg gcgccccttg gcgccctgga tggagacgcg gcccaccgga tggctccggc
     8881 tgttcgccct cggcgcgttc ctgccgctcg cgatcgtcct ggccgccggg tccggcgcgg
     8941 cggcgcacga caacgcgttc atccgcctgg accgcgccct ctacaccctg gccgtctcgt
     9001 ggatcttcct gagcgctcgg ctttcggtgg gggagctgct ccaccgctgg atccgccgcg
     9061 cgcggatccg caccaagctg ctcgtcgcgt tcgggatctt cgcggtgacc ccggcgctcc
     9121 tggcgttcac gtacgcgacc ctcgccggat ggatccacgc cggcagcctc cgcgcctcgg
     9181 tgatcgagcg ccagctgggg gcccgttcgg gggggcgtgg gctgatcgcc cgcgcgcggg
     9241 agggcgctcc tccggccagc ggagcggagc tggcggcgcg gatcgaacgc gagcgccccg
     9301 ttctcgtgga tcgcggcctg gcggcggtgg ccctcgagcg cgggcccgaa ggatggcgaa
     9361 cgatcgcgag ctacggcgat cccgactcgc tcttccgtcc cgcggccgcg ccggtcgccg
     9421 actcgggcga cgtggtgcag ggactggtct atcgaaaccg ccgcttctgg tgggcggaga
     9481 cggcgctctg gccgaagggc ggcgacacgc tcgccctctt gacctatgag ccgatcgaca
     9541 ccacgcgcgt gagccggctg gcccgggaca tgggatgcga cgcggtgctc atgacgtcga
     9601 cgaccatgtt ctcgggagga accaaggtcg aggtggggcc gaaacggcgc aatcagcgcc
     9661 acttccgcat ggccggaggc atcgtcgaga tcggcacgga cgagccgttg cccgatgggg
     9721 tctccgactc cgtcgcgctc gactcgctct ccagtctcgg cggcctgtcc ctggtcggcg
     9781 ggggagacta cgcgaccgcg acgtcgctca accagatccg caagacgtcg aacggcgggt
     9841 ccacgccgct ctgctatctc tggaggggcg gcgaatggca tcgcggggcc gccctgctcc
     9901 tggtccactc gccgccgggg gagtcattcc agtactccgg aatggacctc ggcccgatcg
     9961 cggtcgccgc tcgtgtcgcg ttgatcgtct tcgcgatcat cttcatctcc ctggagatcg
    10021 tatcgctggt ggtgggcgct cgggtcgcgc gcttcatcac gcgcggcgcg gccgggctgc
    10081 gccaggcggc ggtatcgatc gggcaggggg atttctcggc gcgcgtgcgg gtcccgtccg
    10141 aggacgagct gggcgagctg gccgattcgt tcaatcgcat ggccgcgggg ctcgaggagg
    10201 ggcagcgcgc gatggttgag cgtgagcaga tgcgccgcga gctggagctg gcgcggcgga
    10261 tccagtcgcg tctcctcccg cccgggccgc cgtccttgcc gcgcctggac gtcgcggccg
    10321 ccaatgcgat gagccagcag gtgggcggcg actactacga cttcatctcg ctcgagaacg
    10381 gacacgtggg gttctgcatc gccgacgtgg ccgggaaggg cgtcgccgcc gcgttattga
    10441 tgagcagcgt gaagaccgcg ctcgtctcga gcgccgcggt cgagaccgcg ccgcacctct
    10501 tgaccggacg ggtgaaccgc ctgctggagc agagcatcga gcccgggcgc ttcgtgacct
    10561 tcttcctcgc gtcgctcgat ccctcgacga tgcgcctgga gtacgtgaac gcgggacacc
    10621 ccgcgccgat gcttctgcgc gccgcgggcg gcgtggagcg gctggagatg ggcggcatca
    10681 ttctcggcat cgatgcggca gccaagttcg aagccggcgc cgtaacgttg gcgcccggag
    10741 acctgctggc gatgttcacg gacggggtga ccgaggccca gggagcggac gaggagctgt
    10801 tcggggacgc gcgcatcgag tcggtgctgc acttgaatcg cgaccgcacc gccgaggagg
    10861 tgctgaacgc gttgatcgaa gcggtgaaga gctacgaggg agcgcgggga ccgtcggacg
    10921 atctcacggc ggttgtggtg aagctggagc gatagctctg gggccctgcg tcactttccc
    10981 gcgacaatcg tccatgattt caccgccgct ggcggcggta aaaagttggg cggtcagagt
    11041 ttgcggcggt ggagactacc gtgcgtacaa ccgatgcgcg gccatgaggt ggcgcagcgc
    11101 gtcggcgcgc tggccgagct tttccagcaa cagtcccagg ttgtagtgcg cgtccgcgag
    11161 atcgggatcc agcgcgatcg cctgctcgta cgcttcggcg gcggcctgcg ggcagtttcg
    11221 atcttccagc aacacgccca gattgaaatg cgacgtcgcg tcctcggggt cgcactggat
    11281 cgcgcggcga tagcgcgcct cggccgccgc gagatctccc gcgcggtgat ccagccggcc
    11341 cagattgagg tgggcgtcgg cgtagttcgg atcggcgtcg agcgctttca gatacgcctg
    11401 ccgggcctcg agcggcgacg tgctctccat ctccgagccc agccggaacc aatgctcggc
    11461 ggactcatcg ggtggcgctt cccgtcgctc tgtcgcccgc ttcgaagcga cgtcgcggaa
    11521 gatggagatc accgccggag cccctgatga tttctgaagc ggagccgcgg gcgtcctgga
    11581 cgatttctga agaggatccg ccggcgtctg ggccgatttc tgaagatggg ccgacggctt
    11641 tccgggccac tcttggggag ccgctgcgac gtcctcgacg ccttcctcct cggctccgcc
    11701 tccggcggac atcacctcga acagcggcgc ctcgctcgcg ccggccacgt cgcccgcgac
    11761 gaaatcgagc acgaactgac ccgattcggg ctgccagggc gccttgccgt cccaggcgat
    11821 cgcgcggtcg ccgtcggcgc ggatccgaat gctgctcagg gggtgttcct cggagagctg
    11881 ctgcttcagg gtggaccaga tgcgcttcac gcgggaggcg ggaacacccg cgtcgagcag
    11941 gcgccgcgtc gtgcggagca ggagcagctc ctggaaggtg aactcgaggg gtccgccggg
    12001 accgtccttg gggacaatgc cgcccatgcg agcccaggtg cggaccctgg gctccgtgac
    12061 gtggagaatc cgcgccgcgt cgcgcatgga gaaggttctc gccgcgtcca ttcccgaccg
    12121 cccctcacga catcccgaca gccctgccac gccccgccga aaatctacac caacgggctg
    12181 ccgtttgctc tgccgaaccg ccctctcgct accgtgccgc ccgtttcgcc gccttcgccg
    12241 gagcttccgc ccgcttc
//
LOCUS       DASSLZ010000360         8793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U1185 k141_1897030, whole genome shotgun sequence.
ACCESSION   DASSLZ010000360 DASSLZ010000000
VERSION     DASSLZ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963341
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 06:19:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,735
            CDSs (total)                      :: 2,700
            Genes (coding)                    :: 2,684
            CDSs (with protein)               :: 2,684
            Genes (RNA)                       :: 35
            tRNAs                             :: 31
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 1 of 16
            Pseudo Genes (multiple problems)  :: 4 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897030"
                     /isolate="SMAG_U1185"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..926)
                     /gene="rsmA"
                     /locus_tag="VFM00_13635"
     CDS             complement(93..926)
                     /gene="rsmA"
                     /locus_tag="VFM00_13635"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET7499334.1"
                     /translation="MPGALRELGVRPSRRLGQNFLQDPRVADRIAALVEDQLTEILEI
                     GPGLGALTERLAALGRRVLVVELDLRLADALERKYDAAGNRAAVRVVRGDILNQRLEE
                     LLPGSGPVTVVANLPYSITTPAIEWVLAQGPRVRRAILMVQREVAERMTARPGGKEYG
                     SLAVFLSLHAEVEPMFRVSPGAFHPRPDVDSTVVRLTPRPYPGTTEAERREAERIARA
                     ATTGRRKTVANALARGLAVEPAAARRLLDEAGIDDSRRGETLAVEEWLALARASLGEA
                     P"
     gene            complement(1018..1779)
                     /locus_tag="VFM00_13640"
     CDS             complement(1018..1779)
                     /locus_tag="VFM00_13640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET7499335.1"
                     /translation="MIDSHAHIGRADFDEDRDLVLARAREAGVTRIVEAGTDAAGSRA
                     AIALARRDPLVRAAVGFHPCDVAAERMAEMDEIEALAAEPEVVAIGETGLDLHWPENA
                     PIEVQEEFLRRHVAIAKKAGKPLVLHHRAAGERVAALLEREGPPSAGGTFHCFAGDVA
                     LARRVIAMGMKIGVGGSATFKKSPLPEILRQIGLEHVVLETDAPYLAPVPHRGKRNEP
                     AYLALVRDHVAASLGTTPAALETATDAAARALFRI"
     gene            complement(1805..3358)
                     /gene="metG"
                     /locus_tag="VFM00_13645"
     CDS             complement(1805..3358)
                     /gene="metG"
                     /locus_tag="VFM00_13645"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7499336.1"
                     /translation="MAKYYLTTAIDYVNAAPHIGTAYEKIAADALARYRRLAGFDTHF
                     LMGNDEHSTNVEKAAREKGLEPREYCDRMAEVFQGVWKRLDISYDDFIRTTEPRHVRG
                     VQAMFERFAERGDLYKGKYEGFYCVSCERFYPEKDLEEGKCPVHHTEPQWLSEENWFF
                     KLSAYGARLLEHIRTHPEFIIPDIRKNEIVNVIEGGLEDISVSRSSTRWGVPFPGDPS
                     QMVYVWFDALINYVSGVGYPDPDGDYARWWPADLHVIGKDITRFHCIIWPAMLLSAGV
                     PLPKTVLGHGWVHFQGQKLSKSLGNIVNPLEVADKYGADPLRYYLLKEVPLSRDGDFT
                     WDLFIDRYNADLANDWGNLFTRTVSMIHRYRGGILARAEPIDGLDGLIADAFRDYRAG
                     FEAFAIERGIEAAWTIVRRANRLVEERAPWNLAKDPARAEELDRLLGALAVALQHTAI
                     LLFPIMPSRARLVWETLRLAPALEEARFPAEGALLPPPPTGVVLGASEPLFPRIDRAV
                     AAGSAASGR"
     gene            complement(3358..4479)
                     /locus_tag="VFM00_13650"
     CDS             complement(3358..4479)
                     /locus_tag="VFM00_13650"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HET7499337.1"
                     /translation="MERTDIAETPAATAVPPAPPASPAPVLEIVFKGRRREHYANVQR
                     LPLREGDYVIVQAERGEDLGRIHHSSEWVSAAIAPEGLKAVLRAARPEDLQRLEANRA
                     REERAFIQCRDRIAQRDLEMKLVDCEFQLDGNRVTFYFTAEKRVDFRELVKDLASIFR
                     TRIELRQIGVRDEAGRIGGVGTCGRELCCATWLREFEPITLKMAKDQGLSPSPSKISG
                     ACGRLKCCLRYELDFYKESAREFPKIGSRLTLEGAEWEVGRVDIFHRTLHLRDDTGKE
                     TTMLLTDVPKGTKIAGPTRDRRKGCDKTRCAARGAAPGDGDSVGEGDAPRPPDSRGPD
                     ARGGNQGHRPHGPRGGHGPNHGGPPQGGPRRGPRPGPER"
     gene            complement(4518..5669)
                     /locus_tag="VFM00_13655"
     CDS             complement(4518..5669)
                     /locus_tag="VFM00_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7499338.1"
                     /translation="MSDDTLRGQDEALALIRAAWKTGHVGHAYLFHGPAGVGKTRASL
                     WFARAMLCERPRPGGEPCDACPSCVAALALRHPDLELLVPLPTFKTEGRTERQADEAR
                     SEARARIRERYAREPWFAPVFSRPVVHSVEDLARAKEFLSLTAQREGGWKVLVVKGAE
                     AMTGPAAHAFLKILEEPQPNRLLILGARQRRALLPTIASRCLPIRFRPLPAATIVEAL
                     TVGYGIAPAAARLAAATAQGSLTRAVAPFLQPMDAAGKEAPDASDFARLRDAAIDLFV
                     RPKTSAVLGPLRKARLERDRGRFLTAVALATHYYRDVIREQVEGPGTEPANFDLKREV
                     AADARALPLETLAARVRILEEIAAAVQSNVTAAYAVASAQNRMGLFARG"
     gene            complement(5681..6919)
                     /locus_tag="VFM00_13660"
     CDS             complement(5681..6919)
                     /locus_tag="VFM00_13660"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7499339.1"
                     /translation="MAALAALLLVALAVAAPAPGAAQASAPEVLAQAAPAAPDSSAPD
                     ASGAEIEAQKKELESLRQELEKRRVISQQLKGRERNILGDLRDTEKNLQLTLRYLAAL
                     ERRRRAVAGDLGETTSELGRTAIQLEADRRRLAWRLREIYKRGRSRDIEYILSARSFG
                     DLVTRTYYLARVAREDHQQLLLTQARRVEVQDTKTRLESRKRELDRLASETEREKRAL
                     AQLTAQRRNLLRQVRSDAKSNEQAQAEIRRASKRIQGLIEQLEKRRLAAERGAPGEQP
                     LFGDFSKNKGRLAWPVTGKVATGFGNVTNPRFGTKTFNSGIDIAAPFGTPIKAVAQGR
                     MDYVNWLEGFGKCAILNHGGGFYTLYAHASEISVPVGKDVAAGEVIGRVGDTGSTIGT
                     ALHFEIRRGKQALNPIEWLR"
     gene            complement(6907..7779)
                     /locus_tag="VFM00_13665"
     CDS             complement(6907..7779)
                     /locus_tag="VFM00_13665"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HET7499340.1"
                     /translation="MMNRARYVSRELWRGIAQHRTLLLTSILSMASILLIFLFFLMII
                     QSVAVYTDQLESREEVSVFLNEGLSRSDLAQVQASLQAIPGVGTLRYISKEEAWAAFR
                     EDVADEALIQAVGSNPLPASIVIRPASGYRSAEGVRAIAREAETVAHVEDVRYAGEWV
                     LRAQQVVGTLKQIGAVIGIIVVLGVLFVVGATTRLSVQARLDSIHLVRSLGGGFLFNE
                     APYLLEGFVLASIASLLTVLVARFLNDSLAEGLFRLQFLPNSTLALFVGVSGLLGLLG
                     SWIAVVTLPRKWLL"
     gene            complement(7776..8486)
                     /locus_tag="VFM00_13670"
     CDS             complement(7776..8486)
                     /locus_tag="VFM00_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7499341.1"
                     /translation="MPLIRLDHVSKTYGDRIALSDVTLSFEPGEWVFVLGPSGAGKST
                     LLRILAMAEKPSSGRVHVDPGEVPGRGGRVSSRKMRRLVGVLGQEFRLLQDRTVHENI
                     ALACQISGIWDRTEIRDRVNPLLDQMGIRGKESLFPSDLSAGEKQRVALARAMARHPR
                     ILIADEPTGNLDPVAATQIFGLLREINARGALVLVATHAEDWVRRHPGRVIRLERGIL
                     RSDAPEGDPSATAEVLPR"
     gene            complement(8536..>8793)
                     /locus_tag="VFM00_13675"
     CDS             complement(8536..>8793)
                     /locus_tag="VFM00_13675"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET7499342.1"
                     /translation="DVGVVVAMYHDQGLIAAKSGGIGGAANVTLGLPFVRSSVDHGTA
                     FDRAWRGGAKAPDPSGLATAVRVGADLATRAGRKRLEWTWP"
BASE COUNT         1263 a         3095 c         3125 g         1310 t
ORIGIN      
        1 ggccgaatcg ggatcgacga agccttcctc ttgagctgcc gcgacggccg ggaatattga
       61 tgtgagaagc agtaccagga gcagccttcg gctcatggag cttctcccaa ggaagcccgc
      121 gccagcgcga gccactcttc caccgcgagc gtctccccgc ggcgcgaatc gtcgattccg
      181 gcttcgtcga gcagccggcg cgcggcggcg ggctccaccg cgaggccgcg agccagcgcg
      241 ttcgcgaccg tcttgcggcg ccctgtcgtc gcggcgcggg cgatgcgctc ggcttcgcgc
      301 cgctcggcct cggtcgttcc gggatagggg cgcggggtca ggcgcaccac cgtggaatcc
      361 acgtcggggc ggggatggaa ggcgccgggc gacacccgga acatcggctc gacctcggcg
      421 tgaagggaaa ggaagacggc gagcgagccg tactccttgc ctccgggcct cgcggtcatc
      481 cgctcggcga cctcgcgctg caccatgagg atggcgcggc gcacgcgggg gccctgcgcg
      541 agcacccact cgatggcggg cgtcgtgatc gaatagggaa ggttggcgac cacggtcacg
      601 ggcccgctcc ccggcaggag ctcctccaag cgctggttca ggatatctcc gcgaaccacg
      661 cgcaccgcgg cccggttccc ggccgcgtcg tatttccgct ccagggcatc ggccagtcgg
      721 aggtccaact ccaccacaag gacccgccgc ccgagggccg ccagccgctc ggtcagggcg
      781 cccaggccgg ggccgatctc gagaatctcg gtcagctggt cctcgacgag cgcggcgatc
      841 cggtcggcca ccctggggtc ctgcaggaag ttctgcccca ggcgccggct gggccggacg
      901 cccagctcgc gaagggctcc gggcacgcca gaggcggccc ccgggtcgcc ggtgcccgcc
      961 gtggggcgga gccgctcgcg ccgcgacgcc cgcgacggcg gcggggccgg agtccgccta
     1021 gatccggaag agcgcacggg ccgcggcatc ggtggccgtc tccagcgccg cgggcgtggt
     1081 cccgagcgag gcggccacgt gatcccgcac gagcgcgaga taggcgggct cgttccgctt
     1141 tccccggtgc ggcaccggag cgaggtacgg ggcgtccgtt tccaaaacca cgtgctccag
     1201 cccgatctgg cgaaggatct ccgggagcgg gctcttcttg aacgtggccg atccgccgac
     1261 cccgatcttc atccccatcg cgatcacgcg ccgcgcgagc gccacgtctc ccgcgaagca
     1321 gtggaacgtt cccccggcgg acggcggtcc ctcgcgctcg aggagcgcgg ccacgcgctc
     1381 tccggcggcg cgatggtgca gcacgagcgg cttccccgcc ttcttcgcga tcgcgacgtg
     1441 gcggcgcagg aactcttcct gcacttcgat cggcgcgttc tccggccagt gcaggtccag
     1501 acccgtctcg ccgatcgcga ccacctccgg ctccgcggcc agggcttcga tctcgtccat
     1561 ctcggccatg cgctcggccg cgacgtcgca gggatggaag ccgacggcgg cgcgaacgag
     1621 cggatccctc cgcgccaagg cgatggcggc gcggctcccg gcggcgtcgg tgccggcctc
     1681 cacgatccgc gttacgccgg cctcgcgcgc gcgggccagc accaggtcgc ggtcttcgtc
     1741 gaagtcggcg cgcccgatat gggcgtgcga gtcgatcatg ggaatggggt acggaccgtg
     1801 ccggtcaacg tccgctcgcg gcgctccccg cggccaccgc gcgatcgatc cgcggaaaga
     1861 gcggctccga ggccccgagc accacgccgg tcggcggcgg cggcaacagc gcgccctccg
     1921 ccgggaatcg cgcttcttcg agggcgggcg caagccggag cgtctcccag acgaggcgcg
     1981 cccgggacgg catgatggga aagaggagga tggccgtgtg ctgcagcgcc acggccagcg
     2041 cgccgagcag gcggtccagc tcctcggcac gcgcgggatc cttggccagg ttccatgggg
     2101 cgcgctcctc cacgagacgg ttggcgcgcc gcacgatggt ccacgcggcc tcgattcccc
     2161 gctcgatggc gaacgcctcg aacccggcgc ggtagtcccg gaacgcatcc gcgatcagcc
     2221 cgtccagccc gtcgatcggc tcggcgcggg ccaggatgcc gccccggtag cgatggatca
     2281 tcgacaccgt gcgcgtgaac aggtttcccc agtcgttcgc caggtcggcg ttgtagcggt
     2341 cgatgaacag atcccaggtg aagtcgccgt cgcgggagag cggcacttcc ttgagcaggt
     2401 agtagcggag cggatcggcg ccgtacttgt ccgccacttc aagcgggttg acgatgttgc
     2461 cgagcgactt ggagagcttc tgcccctgga agtgcaccca cccgtggccc agcaccgtct
     2521 tcggcagcgg cacgccggcg ctcaggagca tcgcgggcca gatgatgcag tggaagcgcg
     2581 tgatgtcctt gccgatcacg tgcaggtcgg ccggccacca gcgcgcgtag tcgccatcgg
     2641 ggtcgggata gccgacgccg ctcacgtagt tgatcagcgc gtcgaaccag acgtagacca
     2701 tctgcgaggg atcgcccggg aagggaacgc cccaccgggt cgaggagcgc gagacggaga
     2761 tgtcctccag cccgccttcg atcacgttca cgatctcgtt cttccggatg tccgggatga
     2821 tgaactccgg atgggtccgg atgtgctcca ggaggcgcgc cccgtaggcg gagagcttga
     2881 agaaccagtt ctcctcgctc agccattgcg gctccgtgtg gtgcacgggg cacttcccct
     2941 cctcgagatc cttctcggga tagaagcgct cgcacgaaac gcagtagaag ccctcgtact
     3001 ttcctttata gaggtcgccc cgctcggcga agcgctcgaa catcgcctga acgccgcgca
     3061 cgtggcgcgg ctcggtggtg cggatgaagt cgtcgtagga gatgtcgagc cgcttccaga
     3121 cgccctggaa cacctcggcc atccggtcgc agtactcccg cggctccagc cccttctcgc
     3181 gcgcggcttt ctcgacgttg gtggagtgct cgtcgttccc catcaggaaa tgggtgtcga
     3241 agccggcgag ccgccggtag cgggcgaggg cgtcggcggc gatcttctcg tacgcggtgc
     3301 cgatgtgggg cgccgcgttc acgtagtcga tcgccgtcgt gaggtagtac ttggccatca
     3361 gcgttctggc ccgggacggg gaccgcggcg cgggccgccc tgcggggggc cgccatggtt
     3421 cgggccgtgc ccgccgcgcg gcccgtgcgg acggtgtccc tggttgccgc cgcgggcgtc
     3481 ggggcctcgg gagtcgggag gccgcggcgc gtcaccctcg cccacgctgt cgccgtcgcc
     3541 gggtgcggcg ccgcgcgcgg cgcagcgcgt cttgtcgcag cccttccgcc ggtcgcgcgt
     3601 cggtccggcg atcttggtcc ccttcggaac gtcggtgagg agcatggtcg tctccttgcc
     3661 cgtatcgtcg cgcaggtgga gcgtgcggtg aaagatgtcc acgcgtccca cctcccactc
     3721 cgccccctcc agcgtgaggc ggcttccgat cttcggaaat tcgcgcgccg attccttgta
     3781 gaagtcgagc tcgtagcgga ggcagcactt gagccggccg cacgcccccg agatcttgga
     3841 tgggctgggc gagagcccct ggtccttcgc catcttgagc gtgatcggct cgaactcgcg
     3901 cagccaggtg gcgcagcaga gctcgcgccc gcacgtgccc acgccgccga tccgtcccgc
     3961 ctcgtcgcgc acgccgatct ggcgcagctc gatccgcgtg cggaagatgg aggccaggtc
     4021 cttcaccagc tcgcggaaat ccacgcgctt ctcggcggtg aagtagaagg tgacccggtt
     4081 tccatccagc tggaattcgc aatccacgag cttcatttcg agatcgcgct gcgcgatccg
     4141 gtcgcggcac tggataaagg cgcgctcctc gcgggcccgg ttcgcctcca ggcgctgcag
     4201 atcctcgggg cgggcggcgc gaagcacggc cttgagcccc tcgggcgcga tcgccgccga
     4261 gacccattcg ctggagtggt ggatgcggcc caggtcctcg ccccgctcgg cctggacgat
     4321 cacgtagtcc ccctcgcgga gcgggagcct ctggacgttc gcgtagtgct cgcggcggcg
     4381 ccctttgaag acgatctcga gaacgggagc gggcgaagcc gggggcgcgg gaggaaccgc
     4441 ggtcgcggcg ggagtctcgg cgatgtcggt gcgttccata tcgattcacg cccgccggag
     4501 cgggccctcg tggacggtca tccgcgcgcg aacaggccca tgcggttctg ggccgacgcg
     4561 acggcatagg cggcggtgac gttcgactgc accgccgcgg cgatctcctc caggatccga
     4621 acccgcgccg cgagcgtttc gagcggcagg gcgcgggcgt ccgccgcaac ctcgcgtttc
     4681 aggtcgaagt tggccggctc ggtgccgggc ccctcgacct gttcgcggat cacgtcgcgg
     4741 taatagtgcg tcgcgagcgc caccgcggtg aggaagcggc cgcggtcgcg ctcgagccgt
     4801 gccttccgga gcggcccgag gacggcgctc gtcttcgggc gcacgaacag gtcgatggcc
     4861 gcgtcccgca gccgggcgaa gtcggacgcg tcgggggctt ccttccccgc cgcgtccatc
     4921 ggctggagga agggcgccac cgcccgggtc aggcttccct gcgccgtcgc cgccgcgagc
     4981 cgcgcggccg ccggagcgat cccatacccc acggtcagcg cttccacgat cgtcgcggcc
     5041 ggaagcggcc ggaagcggat cgggaggcat cgcgaggcga tcgtcggaag gagcgcgcgc
     5101 cgctgccgcg ctcccaggat cagcaggcga ttcggctgcg gctcttcgag gatcttcaag
     5161 aaagcgtgcg ccgcgggtcc ggtcatcgcc tcggccccct tcacgacgag caccttccag
     5221 cccccctcgc gctgcgccgt cagcgacagg aactccttcg cacgcgcgag atcctccacc
     5281 gagtgcacga cggggcgcga gaagaccggc gcgaaccagg gctcccgggc gtagcgctcg
     5341 cggatgcggg cgcgcgcctc ggagcgcgcc tcgtcggcct gccgctcggt gcgcccctcg
     5401 gtcttgaagg tggggagcgg aacgagaagc tcgaggtcgg gatggcggag cgccaaggcc
     5461 gcgacgcagg aaggacacgc gtcgcaaggc tcgccgccgg gccggggccg ctcgcacagc
     5521 atggcgcgcg cgaaccagag cgaggcgcgc gtcttcccca cccccgccgg accatggaac
     5581 aggtaggcgt ggccgacgtg gcccgtcttc caagcggcgc ggatcaaggc gagcgcctcg
     5641 tcctggccgc gaagggtgtc gtcgctcatt gggtgaacgg ctatcggagc cactcgatcg
     5701 ggttcagcgc ctgcttcccc cggcgaatct cgaagtggag tgccgttcca atcgtggagc
     5761 cggtgtcgcc gacccggccg atgacttcgc cggcggcaac atccttgccg accggcacgc
     5821 tgatctcgga ggcatgtgcg tacagggtgt agaagcctcc tccgtggttg aggatagcac
     5881 acttgccgaa gccctcgagc cagttgacgt aatccatccg cccctgcgcc acggccttga
     5941 tcggggtccc gaagggggcc gcgatgtcga tgccgctgtt gaaggtcttg gtcccgaagc
     6001 gcggattggt cacgttcccg aacccggtcg cgaccttgcc cgtcaccggc caggccaggc
     6061 gccccttgtt cttggagaag tccccgaaga gaggttgctc gccgggcgcc ccgcgctcgg
     6121 ccgccaggcg ccgcttctcc agctgctcga tcagcccctg gatccgcttg ctcgcccgcc
     6181 ggatctcggc ctgggcctgc tcgttcgact tggcgtcgga gcggacctgc cggaggaggt
     6241 tccgccgctg cgcggtgagc tgcgccagcg cccgcttctc gcgctcggtc tcggaagcca
     6301 gccggtccag ctcgcgcttc cggctctcga gccgcgtctt ggtgtcctgc acctcgaccc
     6361 ggcgcgcctg cgtgaggagc agctgctggt ggtcctcgcg cgccacgcgc gcgaggtaat
     6421 aggtccgcgt caccaggtcg ccaaaggagc gggccgagag gatgtactcg atgtcgcggc
     6481 tccgcccccg cttgtagatc tcgcggaggc gccaggccag gcgccgccgg tccgcctcca
     6541 gctggatcgc cgtgcgcccc agctcggagg tcgtctcccc caggtcgccc gccaccgcgc
     6601 ggcggcggcg ctccagcgcg gccaggtagc gcagcgtgag ctggaggttc ttttcggtgt
     6661 cgcgcaggtc gcccaggatg ttccgctcgc gtcccttcag ctgctgggag atcacgcggc
     6721 gcttctccag ctcctggcgg agggactcca gctccttctt ctgggcttcg atctcggcgc
     6781 cgctcgcgtc gggcgccgag ctgtcgggag ccgccggagc cgcctgggcg aggacttcgg
     6841 gcgcggaggc ttgcgcggcg ccgggtgccg gcgcggcgac cgccagggcg acgagaagga
     6901 gcgcggctag agcagccatt tcctcggaag cgtcacgacc gcgatccagc tcccgaggag
     6961 tcccagaagc ccgctcaccc cgacgaagag cgcgagggtc gagttgggga ggaattgcag
     7021 ccggaagagt ccctcggcga gcgagtcgtt caggaatcgc gcgaccagga ccgtcaggag
     7081 cgaagcgatc gaagcgagga cgaatccctc gaggagatag ggggcttcgt tgaagaggaa
     7141 tccgccgccg agcgagcgga ccaggtggat cgagtcgagc cgcgcctgca ccgagagacg
     7201 ggtcgtggcg cccacgacga agagaacgcc cagcacgacg atgatcccga tcaccgcgcc
     7261 gatctgcttc agcgtgccga ccacctgctg cgcgcggagc acccactccc cggcatagcg
     7321 cacgtcctcg acgtgcgcga ccgtctcggc ctcccgcgcg atggcgcgca ctccctcggc
     7381 gctccgatag ccggacgcgg gtcggatcac gatcgaggcg ggaagcgggt tcgagccgac
     7441 tgcctggatc agcgcctcgt cggcgacgtc ctcacggaag gcggcccacg cctcctcttt
     7501 gctgatgtag cgaagcgtcc ccacgccggg gatcgcctgc agggaggcct gcacctgcgc
     7561 cagatcggag cgcgagagac cctcgttcag gaagaccgag acctcctcgc gcgattcgag
     7621 ctggtcggtg tagaccgcca cgctctggat gatcatgagg aagaagagga agatgaggag
     7681 gatcgacgcc atcgagagga tgctcgtgag gagcagcgtg cgatgctgcg cgatcccccg
     7741 ccacagctcc cggctgacgt agcgcgcgcg attcatcatc ggggcagcac ctccgcggtc
     7801 gcggacggat cgccttcggg ggcgtccgac cggaggatgc cgcgctccag gcggatcacg
     7861 cgcccggggt ggcgccgcac ccagtcttcg gcatgcgtcg cgaccaggac gagggcgccg
     7921 cgcgcgttga tctcgcgcaa aaggccgaag atctgcgtcg ccgcgacggg atcgaggttt
     7981 ccggtcggct cgtcggcgat caggatgcgc ggatggcgcg ccatcgcccg cgcgagcgcg
     8041 acccgctgct tttcgcccgc ggagagatcg ctcggaaaga gcgactcctt gccgcggatt
     8101 cccatctggt cgaggagcgg gttcacgcgg tcacggatct ccgtgcggtc ccagattccg
     8161 gagatctggc aggcgagcgc gatgttctcg tggacggttc gatcttgaag gagccggaac
     8221 tcctggccga gcacgccgac caggcggcgc atcttgcggc tcgagacccg cccgccgcgc
     8281 ccgggcacct cgcccggatc cacgtggacg cgcccgctcg agggcttttc ggccatggcg
     8341 aggatgcgga gcagggtcga cttgccggcg ccgctgggcc ccaggacgaa gacccattcg
     8401 cccggctcga acgagagggt gacgtcgctc agggcgatcc ggtccccgta ggtcttggag
     8461 acgtggtcca gccggatcag cggcatggca cctctcctcc cacgccgacg cgaggacggg
     8521 cagggcgcgc ggcggtcagg gccatgtcca ctccagacgc ttccgcccgg cgcgcgtcgc
     8581 gagatcggcc ccgacccgaa cggccgtcgc gagccccgag ggatcgggcg cctttgcgcc
     8641 gccgcgccac gcgcggtcga acgcggtgcc gtggtccacg ctgctgcgca cgaagggaag
     8701 gcccagcgtc acgttggcgg ctccgccgat cccgccgctc ttggccgcga tcaagccttg
     8761 atcgtgatac atggcgacga ccacgcccac gtc
//
